Simple method for preparation of cryoprecipitate (CP) and cryodepleted plasma (CDP).
Cryoprecipitates (CP) and cryodepleted plasma (CDP) were prepared from fresh frozen plasma (FFP). Plasma was easily and cleanly frozen at -50 degrees C using a methanol-bath Ultracryostat, which has been available commercially for the past few years. From a random sample (n = 6), factors VIII:C, IX:C, V:C, fibrinogen, antithrombin III and fibronectin were determined. Concerning the total contents and the in-vitro-recovery of factor VIII:C (x:104 IU/53.5%) and fibrinogen x:175 mg/36.9%), the preparation method was as efficient as other equally common methods. Apart from the well-known applications, CP may be used for the substitution of fibronectin (x:46.2 mg/73.0%). The supernatant plasma of cryoprecipitation (CDP) can be utilized for substitution of coagulation disorders especially deficiencies of the prothrombincomplex and antithrombin III (x:IX:C:183 IU/76.2%; V:C:140.5 U/73.8%; AT III:162 U/80.2%).
D000990:Antithrombin III / Q000032:analysis; D011232:Chemical Precipitation; D005164:Factor IX / Q000032:analysis; D005165:Factor V / Q000032:analysis / Q000276:immunology; D005169:Factor VIII / Q000032:analysis; D005340:Fibrinogen / Q000032:analysis; D005353:Fibronectins / Q000032:analysis; D005615:Freezing; D006467:Hemophilia A / Q000628:therapy*; D006801:Humans; D010952:Plasma Substitutes / Q000138:chemical synthesis*; D018805:Sepsis / Q000628:therapy*; D014842:von Willebrand Diseases / Q000628:therapy*
D005353:Fibronectins; D010952:Plasma Substitutes; C035876:factor V clotting antigen; D000990:Antithrombin III; D005165:Factor V; D005169:Factor VIII; D005164:Factor IX; D005340:Fibrinogen