Iodination of thyroliberin by use of Iodogen.
In this method of labeling thyroliberin for radioimmunoassay, 1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril (Iodogen) is used as oxidant. By cation-exchange chromatography and in response to antibody, thyroliberin so labeled behaved similarly to that produced by the conventional Chloramine-T method. Major advantages of the Iodogen method are its reliability, simplicity, and higher yield of stable RIA label in its most convenient (diiodinated) form.
D002852:Chromatography, Ion Exchange; D007202:Indicators and Reagents / Q000378:metabolism*; D011863:Radioimmunoassay / Q000379:methods; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism*; D014508:Urea / Q000031:analogs & derivatives* / Q000378:metabolism
D007202:Indicators and Reagents; C012385:1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril; D013973:Thyrotropin-Releasing Hormone; D014508:Urea