Peptostreptococcus productus strain that grows rapidly with CO as the energy source.
Anaerobic bacteria were enriched with a sewage digestor sludge inoculum and a mineral medium supplemented with B-vitamins and 0.05% yeast extract and with a 50% CO-30% N2-20% CO2 (2 atm [202 kPa]) gas phase. Microscopic observation revealed an abundance of gram-positive cocci, 1.0 by 1.4 micron, which occurred in pairs or chains. The coccus, strain U-1, was isolated by using roll tubes with CO as the energy source. Based on morphology, sugars fermented, fermentation products from glucose (H2, acetate, lactate, and succinate), and other features, strain U-1 was identified as Peptostreptococcus productus IIb (similar to the type strain). The doubling time with up to 50% CO was 1.5 h; acetate and CO2 were the major products. In addition, no significant change in the doubling time was observed with 90% CO. Some stock strains were also able to use CO, although not as well. Strain U-1 produced acetate during growth with H2-CO2. Other C1 compounds did not support growth. Most probable numbers of CO utilizers morphologically identical with strain U-1 were 7.5 X 10(6) and 1.1 X 10(5) cells per g for anaerobic digestor sludge and human feces, respectively.
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000693:Anaerobiosis; D002245:Carbon Dioxide / Q000378:metabolism; D002248:Carbon Monoxide / Q000378:metabolism*; D004734:Energy Metabolism; D006859:Hydrogen / Q000378:metabolism; D010462:Peptostreptococcus / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D012722:Sewage
D000085:Acetates; D012722:Sewage; D002245:Carbon Dioxide; D002248:Carbon Monoxide; D006859:Hydrogen; D019342:Acetic Acid