Reversed-phase high-performance liquid chromatographic determination of mitomycin C in human serum.
A reversed-phase high-performance liquid chromatographic method is presented by which the cancer chemotherapeutic agent, mitomycin C, may be measured in human serum. A mobile phase of methanol:water (35:65) passed through a mu-Bondapak C-18 column at a rate of 1.0 ml/min produced a sharp, symmetrical band for mitomycin C. An improved serum extraction procedure, using a reversed-phase sample preparation cartridge, proved to be efficient and reproducible. Recovery over a concentration range of 10-100 ng/ml was 81.6% with a between-day coefficient of variation of 4.6% (n = 5). The within-day coefficient of variation at 50 ng/ml was 5.6% (n = 10). Ultraviolet detection at a wavelength of 365 nm was sensitive to serum concentrations of 10 ng/ml. Serum concentration-time course data from lung cancer patients receiving mitomycin C by rapid intravenous injection are presented.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007275:Injections, Intravenous; D007700:Kinetics; D008175:Lung Neoplasms / Q000097:blood; D016685:Mitomycin; D008937:Mitomycins / Q000008:administration & dosage / Q000097:blood*; D013048:Specimen Handling
D008937:Mitomycins; D016685:Mitomycin