Improved haemagglutination reagent for detecting antibodies to insulin in diabetic and non-diabetic young and old patients.
A stable reagent of insulin-coated erythrocytes was developed for easy measurement of antibodies to insulin. For this measurement, the principle of graded haemagglutination (GH) was successfully applied. All of 100 healthy, young- to middle-aged controls, had no detectable antibodies. The incidence of antibodies was not higher in diabetic patients injected with highly purified porcine insulin, than in those not injected. A high incidence of antibodies to insulin was found among chronic elderly patients, amounting to 41% of the symptomatically non-diabetic and 77% of the diabetic.
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D002648:Child; D002675:Child, Preschool; D003920:Diabetes Mellitus / Q000276:immunology*; D005951:Glucose Tolerance Test; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006801:Humans; D005779:Immunodiffusion; D007202:Indicators and Reagents; D007330:Insulin Antibodies / Q000032:analysis*; D008875:Middle Aged
D007202:Indicators and Reagents; D007330:Insulin Antibodies