Action of arachidonic acid in ureteral-ligated kidney of the anesthetized canine: possible lipoxygenase activity.
In anesthetized dogs 48 h after unilateral ureteral ligation, intra-arterial injection of arachidonic acid produced a transient increase followed by a prolonged decrease of resistance in the ureteral-ligated kidney; whereas, in the control kidney, only the prolonged decrease in resistance was observed in response to arachidonate. Indomethacin blocked not only the arachidonate-induced renal efflux of both immunoreactive 6-keto-prostaglandin F1 alpha and thromboxane B2 but also vasodilation in both kidneys. In contrast, the initial vasoconstriction in the obstructed kidney was not affected by pretreatment with the cyclo-oxygenase inhibitor. Infusion of 5,8,11,14-eicosatetraynoic acid, an inhibitor of lipoxygenase activity, into the ureteral-ligated kidney after indomethacin markedly reduced the initial vasoconstrictor response to arachidonate. These data demonstrate that vascular reactivity to arachidonic acid is altered in the ureteral-obstructed kidney and are consistent with the hypothesis that formation of lipoxygenase as well as cyclooxygenase derivatives may participate in the hemodynamic responses to arachidonic acid in this pathophysiologic model.
D015117:5,8,11,14-Eicosatetraynoic Acid / Q000494:pharmacology; D015121:6-Ketoprostaglandin F1 alpha / Q000494:pharmacology; D000758:Anesthesia; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism / Q000494:pharmacology*; D004285:Dogs; D006439:Hemodynamics / Q000187:drug effects; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D008084:Lipoxygenase / Q000378:metabolism*; D008297:Male; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D012079:Renal Circulation / Q000187:drug effects; D013929:Thromboxane B2 / Q000494:pharmacology; D014513:Ureter / Q000502:physiology*; D014664:Vasodilation / Q000187:drug effects
D001095:Arachidonic Acids; D015117:5,8,11,14-Eicosatetraynoic Acid; D016718:Arachidonic Acid; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases