Transfer RNA chromatography on reversed phase five: effect of cadmium ion on a queuine-type tRNA.
A sensitive method is described that detects an alteration in the structure of tRNA that is caused by cadmium but not by magnesium or zinc ions. The chromatographic system, RPC-5, separates Drosophila tyrosyl-tRNA into two fractions. These two isoacceptors differ by a single position in the anticodon where either a guanosine or queuine resides. Cadmium ions apparently interact with the tRNA and prevent the chromatographic separation. This is the first instance where cadmium is shown to cause a selective change in nucleic acid structure. The RPC-5 system seems to be uniquely useful in detecting such a change.
D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D019256:Cadmium Chloride; D002712:Chlorides; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004331:Drosophila melanogaster; D006147:Guanine / Q000031:analogs & derivatives* / Q000032:analysis; D009690:Nucleic Acid Conformation; D012346:RNA, Transfer, Amino Acyl / Q000302:isolation & purification* / Q000378:metabolism; D015032:Zinc / Q000494:pharmacology; D017967:Zinc Compounds
D002712:Chlorides; D012346:RNA, Transfer, Amino Acyl; D017967:Zinc Compounds; D002104:Cadmium; D006147:Guanine; C019549:queuine; C016837:zinc chloride; D015032:Zinc; D019256:Cadmium Chloride