Influence of lectin-free interleukin-2 on natural and antibody-dependent cellular cytotoxicity.
Supernatants derived from MLA-144, a gibbon T cell line that constitutively releases interleukin-2 (IL-2) and which lack detectable interferon (IFN) had the capacity to enhance the natural killer (NK) cells function of human peripheral blood lymphocytes. Percoll fractionation revealed that like IFN-inducible cytotoxic cells lymphocytes responding to MLA-144 supernatants cofractionate with native NK cells. Exposure to MLA-144 conditioned medium also potentiated the cytotoxic capacity of extravascular effector cells which are either unresponsive or weakly responsive to interferon-alpha (IFN-alpha). Moreover lymphocyte (K cell)-mediated antibody-dependent cellular cytotoxicity (ADCC) which is unresponsive to IFN-alpha was in most cases subject to a modest potentiation following treatment with MLA-144 supernatants. These data confirm previous reports that IL-2 can regulate peripheral blood NK cell function and also demonstrate that this lymphokine can also influence those natural cytotoxic mechanisms which are unresponsive to IFN-alpha.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D004768:Enterotoxins / Q000494:pharmacology; D006801:Humans; D006922:Hylobates; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000276:immunology* / Q000302:isolation & purification; D007694:Killer Cells, Natural / Q000276:immunology; D037102:Lectins
D004768:Enterotoxins; D007370:Interferon Type I; D007376:Interleukin-2; D037102:Lectins; C026066:enterotoxin A, Staphylococcal; D007371:Interferon-gamma