Preparation of autoxidized cholesterol mixtures from [4-14C] cholesterol and cholesteryl fatty acid esters.
Autoxidation of non-esterified cholesterol, in the solid state, at 100 degrees C, is known to be a relatively slow reaction. The presence of carefully chosen cholesteryl esters considerably increases the ratio of autoxidation. Using this method, mixtures of autoxidized free cholesterol (oxycholesterol) labelled on carbon 4 can be obtained almost quantitatively, in the presence of benzoyl peroxide. The ratio of [4-14C] ester produced by transesterification at the end of the reaction is about 10%.
D016718:Arachidonic Acid; D001095:Arachidonic Acids; D002784:Cholesterol; D002788:Cholesterol Esters; D004951:Esterification; D005227:Fatty Acids; D019787:Linoleic Acid; D008041:Linoleic Acids; D019301:Oleic Acid; D009829:Oleic Acids; D010084:Oxidation-Reduction; D013229:Stearic Acids
D001095:Arachidonic Acids; D002788:Cholesterol Esters; D005227:Fatty Acids; D008041:Linoleic Acids; D009829:Oleic Acids; D013229:Stearic Acids; D016718:Arachidonic Acid; D019301:Oleic Acid; C031183:stearic acid; D002784:Cholesterol; D019787:Linoleic Acid