The reliability of histochemical fibre typing of human necropsy muscles.
The reliability of muscle fibre typing of post mortem specimens was investigated with special reference to the influence of time and temperature. In specimens stored at +4 degrees C, muscle fibre typing could be reliably performed up to at least ten and fifteen days post mortem for the masseter and biceps brachii muscles respectively. The corresponding figures for storage at room temperature were three and six days. The difference in the preservation of enzyme activity between masticatory and limb muscles might be related to the demonstrated difference in the fibre type composition and thus the enzyme content and energy sources.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000328:Adult; D003080:Cold Temperature; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008410:Masticatory Muscles / Q000201:enzymology / Q000473:pathology; D009132:Muscles / Q000201:enzymology / Q000473:pathology*; D009246:NADH Tetrazolium Reductase / Q000378:metabolism; D011180:Postmortem Changes
D009246:NADH Tetrazolium Reductase; D000251:Adenosine Triphosphatases