Deuterium magnetic resonance study of the interaction between chondroitin sulfate and human plasma low density lipoproteins.
[2H]Chondroitin sulfate was prepared by partial N-deacetylation of chondroitin sulfate (via hydrazinolysis) followed by treatment with [2H6]acetic anhydride. 2H NMR spectra of [2H]chondroitin sulfate in the presence of human plasma low density lipoprotein provide evidence for a soluble complex stoichiometry of 3 (and possibly 2) lipoproteins per polysaccharide molecule, and allow a rough estimation of the dissociation constant Kd.
D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000097:blood*; D003903:Deuterium; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D011485:Protein Binding
D008077:Lipoproteins, LDL; D002807:Chondroitin; D002809:Chondroitin Sulfates; D003903:Deuterium