Antibodies to a synthetic peptide corresponding to the N-terminal end of mouse gamma interferon (IFN gamma).
Antibodies to an N-terminal synthetic peptide of mouse gamma interferon (MoIFN gamma) neutralized the antiviral activity of MoIFN gamma but not MoIFN alpha/beta, human IFN alpha (HuIFN alpha), HuIFN beta or cynomologus monkey IFN gamma (CynIFN gamma). Comparatively, antibodies to mouse N-terminal synthetic peptide showed only 10% reciprocal cross-reactivity in neutralization tests against heterologous HuIFN gamma and 13% cross-reactivity in the ELISA test against Hu N-terminal peptide. The predetermined specificity of these antibodies make them powerful tools for studying antigenic relatedness and biological properties of IFN gamma s.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology*; D051379:Mice; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000276:immunology*; D011817:Rabbits
D000906:Antibodies; D010446:Peptide Fragments; D007371:Interferon-gamma