[Change in the structure of fibroblast chromatin in a patient with Down's disease and his mother].
A clear-cut difference between chromatin melting curves of cells from the patient with Down's disease and his mother and ones from healthy individuals and the proband's father was shown by fluorescence microscopy and acridine orange staining on human fibroblasts incubated in autologous serum. These data suggest the presence of an obvious genetic correlation between phenotypically healthy mother and her sick child. The identity of the chromatin structure of the patients detected both on lymphocytes and other type of cells, human fibroblasts, allows a suggestion that the phenomenon of the altered chromatin structure is typical generally of the given individual's tissues. Certain changes in the cell chromatin structure are mediated by the effect of autologous serum.
D000165:Acridine Orange / Q000378:metabolism; D002843:Chromatin / Q000032:analysis*; D004247:DNA / Q000378:metabolism; D003720:Densitometry; D004314:Down Syndrome / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000032:analysis*; D006651:Histocytochemistry; D006801:Humans; D008214:Lymphocytes / Q000032:analysis; D008297:Male; D008856:Microscopy, Fluorescence; D011489:Protein Denaturation; D013696:Temperature
D002843:Chromatin; D004247:DNA; D000165:Acridine Orange
Izmenenie struktury khromatina fibroblastov u bol'nogo bolezn'iu Dauna i ego materi.