Electric birefringence of eukaryotic ribosomes.
Eukaryotic 60S ribosomal subunits were studied by transient electric birefringence. Conformations of subunits in active and inactive states upon changes in magnesium concentration were compared by electric birefringence and orientational relaxation time measurements. Active subunits exhibit a positive birefringence and a relaxation time of the order of 8 microseconds. In the presence of EDTA, inactive subunits show no birefringence. When Mg2+ is reverted in the cold to its initial level, the electro-optical properties of the subunits are partially restored although the particles remain biologically inactive.
D000818:Animals; D001718:Birefringence; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D004492:Edetic Acid / Q000494:pharmacology; D008099:Liver / Q000648:ultrastructure; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012270:Ribosomes / Q000187:drug effects / Q000648:ultrastructure*
D015636:Magnesium Chloride; D004492:Edetic Acid; D008274:Magnesium