Effects of D-valine on pulmonary artery endothelial cell morphology and function in cell culture.
The effects of D-valine on the cell culture of bovine pulmonary artery endothelial cells were studied using D-valine-modified Minimal Essential Medium (MEM). D-Valine-treated cultures (46-920 mg/l) were compared with replicate cells grown in L-valine (46 mg/l)-MEM. All media were supplemented with 15% fetal bovine serum (FBS). Endothelial cells were grown for 14 passages with split ratios varying from 1:3 to 1:6. Unlike cells grown in L-valine MEM, cells grown in D-valine MEM did not become contaminated by the growth fibroblasts in primary cultures. D-Valine-treated cells were found to grow in cobblestone array, exhibit contact inhibition and strongly express factor-VIII antigen (F-VIII). D-Valine-grown cells produced PGI2 in greater proportion to PGE2, both constitutively and when stimulated by bradykinin, on comparison with cells grown in L-valine. In addition, cells grown in L-valine, although able to express factor VIII, were not comparable to D-valine cells with respect to other parameters assayed (morphology and growth as a monolayer).
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004727:Endothelium / Q000166:cytology / Q000187:drug effects*; D005169:Factor VIII / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects; D011651:Pulmonary Artery / Q000166:cytology; D014633:Valine / Q000494:pharmacology*
D001095:Arachidonic Acids; D016718:Arachidonic Acid; D005169:Factor VIII; D014633:Valine