Lectins modulate prostaglandin E2 production by rat peritoneal macrophages.
The effect of Aloctin A (Alo A), a lectin having anti-inflammatory activities, on prostaglandin (PG) E2 production by activated rat peritoneal macrophages was compared with that of concanavalin A (Con A), wheat germ agglutinin (WGA), plsum sativum agglutinin (PSA) and soybean agglutinin (SBA). Alo A, WGA, Con A and PSA at 10 micrograms per ml inhibited PG E2 production. But SBA, even at a dose of 1 microgram per ml, stimulated PG E2 production. The inhibition by Alo A treatment of the release of radioactivity from (3H)arachidonic acid-labeled macrophages and the stimulation of this release by SBA treatment were observed. The uptake of (51Cr)-labeled sheep red blood cells by the macrophage was inhibited by Alo A, Con A, and PSA, all at 10 micrograms per ml and SBA at 1 microgram per ml, however, WGA at 10 micrograms per ml stimulated the uptake of the sheep red blood cells. The mechanism of the anti-inflammatory properties of Alo A was discussed.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D015232:Dinoprostone; D004912:Erythrocytes / Q000378:metabolism; D037102:Lectins / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D037121:Plant Lectins; D011458:Prostaglandins E / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D012756:Sheep
D000893:Anti-Inflammatory Agents; D001095:Arachidonic Acids; D037102:Lectins; D037121:Plant Lectins; D011458:Prostaglandins E; D016718:Arachidonic Acid; C033193:aloctin A; D015232:Dinoprostone