Type I estrogen receptors cannot be histochemically localized in frozen tissue sections using estradiol-albumin-FITC conjugates.
Only few investigators still report in literature that estradiol type I receptors can be histochemically visualized using estradiol-albumin-FITC conjugates (E2-BSA-FITC). Most of them report that it is not likely that fluorescence is due to conjugate--type I receptor interaction. It is assumed that rather type II receptors are involved. In this study clear evidence is presented that type I receptors are not visualized. There is some indication that specific staining is only obtained when type II binding sites are present.
D000818:Animals; D002417:Cattle; D002606:Charcoal / Q000494:pharmacology; D003600:Cytosol / Q000032:analysis; D005260:Female; D005629:Frozen Sections; D011485:Protein Binding; D011863:Radioimmunoassay; D011959:Receptors, Estradiol; D011960:Receptors, Estrogen / Q000032:analysis*; D014599:Uterus / Q000032:analysis*
D011959:Receptors, Estradiol; D011960:Receptors, Estrogen; D002606:Charcoal