A simple and rapid solid phase enzyme-linked immunoadsorbence assay for screening monoclonal antibodies to poorly soluble proteins.
An ELISA is described in which protein antigens are fixed to microtitre wells. This assay is of particular use when antigens are soluble only in solutions containing SDS or other detergents, or else have been purified by preparative SDS polyacrylamide gel electrophoresis. Because the protein antigens are fixed to the microtitre wells, it is also possible to re-use antigen derivatised plates several times.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology; D003001:Cloning, Molecular; D003902:Detergents; D004797:Enzyme-Linked Immunosorbent Assay; D005976:Glutaral; D009857:Oncogenes; D011506:Proteins / Q000276:immunology*; D012995:Solubility
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D003902:Detergents; D011506:Proteins; D005976:Glutaral