Local production of Ia-inducing activity in experimental immunogenic uveitis.
The production of soluble lymphokine products of T lymphocytes was studied in the aqueous of rabbits with uveitis induced by intravitreal injection of 2.0 mg of ovalbumin. Aqueous from uveitic eyes demonstrated Ia-inducing activity at a dilution of 1:50 which diluted out at 1:250. The control aqueous and sera from normal or uveitic animals were negative. While production of interleukin 2(IL-2) in uveitic eyes could not be demonstrated in vitro, control rabbit concanavalin A-induced supernatants of nylon-wool-purified T cells contained both Ia-inducing and IL-2 activity. These data demonstrate antigen-driven production of a lymphokine activity in the aqueous of the uveitic eye. Local production of lymphokine may be a potent mechanism for amplification of the inflammatory process in uveitis.
D000818:Animals; D001082:Aqueous Humor / Q000276:immunology; D002478:Cells, Cultured; D005802:Genes, MHC Class II; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D008222:Lymphokines / Q000032:analysis; D008264:Macrophages / Q000276:immunology*; D010047:Ovalbumin; D011817:Rabbits; D012016:Reference Values; D013601:T-Lymphocytes / Q000276:immunology*; D014605:Uveitis / Q000139:chemically induced / Q000276:immunology*
D000949:Histocompatibility Antigens Class II; D008222:Lymphokines; D010047:Ovalbumin