Phosphoproteins of chicken bone matrix. Proof of synthesis in bone tissue.
Twelve-day-old embryonic chick mandibles were cultured in vitro for 6 days. Measurements of the weights of the explants, their mineral and protein components, and the EDTA-extractable proteins established that bone tissue synthesizes O-phosphoserine- and O-phosphothreonine-containing phosphoproteins which are similar to those present in embryonic and postnatal chicken bone matrix. The synthesis of the phosphoproteins was further confirmed by the demonstration that radioactively labeled O-phosphoserine and O-phosphothreonine were identified in bone and in the EDTA-extractable phosphoproteins after pulse-labeling chick mandibles in vitro with radioactively labeled serine and threonine, respectively.
D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D002642:Chick Embryo; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004492:Edetic Acid; D010750:Phosphoproteins / Q000096:biosynthesis*; D010768:Phosphoserine / Q000032:analysis; D010769:Phosphothreonine / Q000032:analysis
D010750:Phosphoproteins; D010769:Phosphothreonine; D010768:Phosphoserine; D004492:Edetic Acid