A mouse monoclonal antibody against rabbit VH allotype shares the predominant idiotype with a rabbit antibody of the same specificity.
A monoclonal antibody, termed 3-2F1, is specific for the rabbit VH al allotypic marker. This antibody appears to recognize a common al determinant since it reacts with all of the a1 immunoglobulin (Ig) that is bound by polyclonal rabbit anti-a1, and completely inhibits the binding of polyclonal anti-a1 to a1 Ig. When tested for the predominant IdX-a1 idiotype previously shown to be expressed on virtually all rabbit anti-a1 antibody, it was found that 3-2F1 also bears this idiotype. In fact, sequential absorption and inhibition assays indicate that 3-2F1 displays the entire set of rabbit IdX-a1 determinants. The results are consistent with the concept that the anti-IdX reagent bears a latent internal image of the common VH a1 epitope.
D000042:Absorption; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001667:Binding, Competitive; D007126:Immunoglobulin Allotypes / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000276:immunology; D051379:Mice; D011817:Rabbits; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D007126:Immunoglobulin Allotypes; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007135:Immunoglobulin Variable Region