Isolation of small molecular forms of Factor VIII/von Willebrand factor from plasma.
Cryoprecipitated factor VIII/von Willebrand factor (FVIII/vWF), freed of fibrinogen by clotting with calcium and Defibrase, was chromatographed on Sepharose CL-2B. Fractions containing lower-molecular-weight forms of FVIII/vWF comprised coprecipitated plasma proteins of similar molecular weights. The major contaminants, fibronectin and IgM, were removed by affinity chromatography on gelatin- and anti-IgM-agarose, respectively. Finally, pure low-molecular-weight FVIII/vWF protein was harvested in the void volume fraction of a Sepharose CL-6B column. The smallest multimers had the size of the tetramer of the basic subunit chain of FVIII/vWF.
D001779:Blood Coagulation Factors / Q000302:isolation & purification*; D011232:Chemical Precipitation; D002845:Chromatography / Q000379:methods; D003080:Cold Temperature; D005169:Factor VIII / Q000302:isolation & purification*; D006801:Humans; D008970:Molecular Weight; D014841:von Willebrand Factor / Q000302:isolation & purification*
D001779:Blood Coagulation Factors; D014841:von Willebrand Factor; D005169:Factor VIII