Functional changes in rat liver mitochondria on administration of 2-methyl-4-dimethylaminoazobenzene.
Administration of 2-methyl-4-dimethylaminobenzene in the diet (0.1%, w/w) for 85-90 days doubled the content of mitochondria in the livers of rats. The azodye was covalently bound to liver proteins, and about 15% of the amount found in liver was associated with the mitochondrial fraction. Mitochondria isolated from the livers of azodye-fed animals showed drastically lowered ability to oxidize NAD+-linked substrates. The inhibited electron-transfer step was the reduction of ubiquinone. The organelles showed a large increase in succinate oxidase activity. The activity of cytochrome oxidase and the content of cytochrome aa3 were substantially higher in these organelles. Azodye-fed animals showed depressed serum cholesterol concentrations. The content of ubiquinone in liver also registered a small increase.
D000818:Animals; D002784:Cholesterol / Q000097:blood; D003580:Cytochromes / Q000378:metabolism; D008297:Male; D008749:Methyldimethylaminoazobenzene / Q000494:pharmacology*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D011506:Proteins / Q000378:metabolism; D051381:Rats; D013367:Submitochondrial Particles / Q000187:drug effects / Q000378:metabolism; D014451:Ubiquinone / Q000378:metabolism; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives*
D003580:Cytochromes; D011506:Proteins; D014451:Ubiquinone; D008749:Methyldimethylaminoazobenzene; D002784:Cholesterol; D004124:p-Dimethylaminoazobenzene