[Chromosomal R-banding with an anti-native DNA (dG-dC specificity) monoclonal antibody].
Chromosomal R-banding was obtained by the use of an anti ds-DNA monoclonal antibody, specific for dG-dC, at an appropriate dilution. Better results were observed when chromosome preparations were trypsinized for 10 sec. with 0.02% trypsin solution. Antibody fixation could be evidence either by immunofluorescent or by immunoenzymatic staining.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002871:Chromosome Banding / Q000379:methods*; D004247:DNA / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D004247:DNA
Marquage chromosomique en bandes R par un anticorps monoclonal anti-ADN natif (spécificité dG-dC).