Spontaneous formation of immunogenic conjugates by 3-hydroxy-para-aminobenzoic acid--a biotransformation product of procaine and para-aminobenzoic acid.
3-Hydroxy-para-aminobenzoic acid (3-HPABA), a biotransformation product of procaine and para-aminobenzoic acid, is capable of binding covalently to proteins by simple incubation at alkaline pH. When administered incorporated in Freund adjuvant to rabbits, 3-HPABA conjugates with normal rabbit serum proteins (3-HPABA-NRS) give rise to both hapten specific antibodies and anti-NRS antibodies. Cross immunological reactions were found between 3-HPABA and ortho-aminophenol (OAP) and 3-hydroxy-sulfanilamide (3-HS) immunological systems, but not between 3-HPABA and para-aminophenol (PAP) and 5-aminosalicyclic acid (5-ASA) immunological systems. The significance of these findings for the explanation of procaine allergy and the possible use of 3-HPABA conjugates for skin testing in procaine hypersensitivity is discussed.
D010129:4-Aminobenzoic Acid; D062365:Aminobenzoates; D000818:Animals; D000906:Antibodies; D001711:Biotransformation; D001798:Blood Proteins; D002352:Carrier Proteins; D003429:Cross Reactions; D004342:Drug Hypersensitivity; D062385:Hydroxybenzoates; D011343:Procaine; D011485:Protein Binding; D011817:Rabbits; D012882:Skin Tests
D062365:Aminobenzoates; D000906:Antibodies; D001798:Blood Proteins; D002352:Carrier Proteins; D062385:Hydroxybenzoates; D011343:Procaine; D010129:4-Aminobenzoic Acid