Calcium-dependent aggregation of human serum amyloid P component. Inhibition by the cyclic 4,6-pyruvate acetal of galactose.
Serum amyloid P component is a normal plasma glycoprotein which is the precursor of amyloid P component, a minor but universal constituent of amyloid deposits. When isolated human P component is exposed to free ionised Ca2+ it aggregates and precipitates. This phenomenon is completely inhibited by the presence of 10(-4)-10(-2) M methyl 4,6-O-(1-carboxyethylidene)-beta-D-galactopyranoside, a recently synthesised specific ligand for amyloid P component. This observation suggests that the autoaggregation of human amyloid P component involves the Ca2+ dependent specific ligand binding property of P component, but does not distinguish between receptor-site-mediated and allosteric mechanisms.
D000682:Amyloid / Q000097:blood / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D011232:Chemical Precipitation; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D008756:Methylgalactosides / Q000494:pharmacology*; D008759:Methylglycosides / Q000494:pharmacology*; D000683:Serum Amyloid P-Component
D000682:Amyloid; D008756:Methylgalactosides; D008759:Methylglycosides; D000683:Serum Amyloid P-Component; C042451:methyl 4,6-O-(1-carboxyethylidene)galactopyranoside; D004492:Edetic Acid; D002118:Calcium