A micromethod for the assay of aldose reductase, its application to pancreatic islets.
A micromethod for the assay of aldose reductase is described. The method, which is based on the fluorometric measurement of the NADP+ formed when an aldose is converted to its corresponding polyol, was applied to lens and pancreatic islet crude homogenates, as well as semipurified lens aldose reductase. The fluorometric method has proved to be reproducible, more rapid, and more sensitive than the classical spectrophotometric procedure, and should find ready application in the screening of potential aldose reductase inhibitors.
D000449:Aldehyde Reductase / Q000032:analysis* / Q000037:antagonists & inhibitors; D000818:Animals; D002417:Cattle; D005470:Fluorometry; D005947:Glucose / Q000378:metabolism; D005985:Glyceraldehyde / Q000378:metabolism; D007515:Islets of Langerhans / Q000201:enzymology*; D007700:Kinetics; D007908:Lens, Crystalline / Q000201:enzymology; D008832:Microchemistry / Q000379:methods; D009249:NADP / Q000032:analysis; D051381:Rats; D013053:Spectrophotometry; D013401:Sugar Alcohol Dehydrogenases / Q000032:analysis*
D005985:Glyceraldehyde; D009249:NADP; D013401:Sugar Alcohol Dehydrogenases; D000449:Aldehyde Reductase; D005947:Glucose