beta-Alanine transport into plasma membrane vesicles derived from rat brain synaptosomes.
Transport of beta-alanine has been demonstrated in membrane vesicles isolated from rat brain, using artificially imposed ion gradients as the sole energy source. The uptake of beta-alanine is strictly dependent on the presence of Na+ and Cl- in the medium, and the process can be driven either by an Na+ gradient (out greater than in) or by a Cl- gradient (out greater than in) when the other essential ion is present. The process is stimulated by a membrane potential (negative inside) as demonstrated by the effect of ionophore valinomycin and anions with different permeabilities. beta-Alanine uptake is inhibited by the presence of GABA.
D000409:Alanine / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism; D008297:Male; D008564:Membrane Potentials; D011188:Potassium / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism*; D013997:Time Factors; D015091:beta-Alanine / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D002712:Chlorides; D015091:beta-Alanine; D005680:gamma-Aminobutyric Acid; D012964:Sodium; D000409:Alanine; D011188:Potassium