Counterimmunoelectrophoresis for identification of equine urine.
Counterimmunoelectrophoresis was evaluated as a method to distinguish urine of human origin from that of equine origin. The procedure used anti-equine serum and anti-human serum antibodies that had been solid-phase absorbed to eliminate species cross-reactivity. Counterimmunoelectrophoresis reliably detected contamination of equine urine by human urine to a level of 10% with a minimum sensitivity to about 2% contamination. Compared with double diffusion, counterimmunoelectrophoresis was approximately 10 to 15 times more sensitive in the detection of urine proteins.
D000818:Animals; D003378:Counterimmunoelectrophoresis; D003429:Cross Reactions; D005260:Female; D006736:Horses / Q000652:urine*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D005779:Immunodiffusion / Q000662:veterinary; D008297:Male; D011507:Proteinuria / Q000652:urine / Q000662:veterinary; D013045:Species Specificity
D007106:Immune Sera