Reduction of DL-selenocystine and isolation of L-seleoncysteine.
Cystine, selenocytsine, and several analogs were reduced by dithiothreitol (DTT), beta-mercaptoethanol (ME) and sodium borohydride (NaBH4). DTT was the most effective; DTT to cystine ratios from 10 to 80 were equally effective. With selenocysteine, however, absorption was considerably reduced at all ratios. Selenocysteine was identified as the reduction product by reaction with Gaitonde's reagent, comparison of absorption spectra, paper chromatograhy, utilization by cysteinyl-tRNA synthetase fro Paracoccus denitrificans and Vigna radiata, changes in solubility after DTT treatment, and comparison of infrared spectra. During the ATP-PPi exchange assay, DTT and ME convert cysteine and selenocysteine derivatives to cysteine and selenocysteine which serve as substrates for cysteinyl-tRNA synthetase.
D000604:Amino Acyl-tRNA Synthetases; D001894:Borohydrides; D002625:Chemistry, Organic; D003545:Cysteine / Q000031:analogs & derivatives*; D003553:Cystine / Q000031:analogs & derivatives*; D004229:Dithiothreitol; D008623:Mercaptoethanol; D008722:Methods; D055601:Organic Chemistry Phenomena; D016566:Organoselenium Compounds; D010084:Oxidation-Reduction; D010231:Paracoccus denitrificans / Q000201:enzymology; D010944:Plants / Q000201:enzymology; D012643:Selenium; D017279:Selenocysteine; D013379:Substrate Specificity
D001894:Borohydrides; D016566:Organoselenium Compounds; D017279:Selenocysteine; C009226:selenocystine; D003553:Cystine; D008623:Mercaptoethanol; D000604:Amino Acyl-tRNA Synthetases; C019975:cysteinyl-tRNA synthetase; D012643:Selenium; D003545:Cysteine; D004229:Dithiothreitol