title: Stoichiometric Tc-99m RBC labeling using stable kit solutions of stannous chloride and EDTA: concise communication.
abstract: An in vitro Tc-99m labeling method is described, which utilizes stable stock solutions of stannous chloride and disodium edetate (EDTA). The kit procedure requires as little as 1 ml of patient blood, can be performed in only 15 min, and gives labeling yields in excess of 98%. By using EDTA, the binding capacity of RBCs for technetium is sufficient to produce Tc-99m RBC doses with specific concentration greater than 50 mCi/ml for first-pass cardiac studies. Scintigrams reveal a slight amount of bladder activity and splenic uptake, but at no time has the thyroid, stomach, or normal bowel been visualized. The predominant 20-hr blood-clearance half-time results in excellent image quality for over 24 hr--an essential property for following intermittent GI bleeding or for performing repeat cardiac function studies over a several-hour time interval.
mesh_terms: D004492:Edetic Acid; D004912:Erythrocytes; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D013667:Technetium; D014001:Tin; D017971:Tin Compounds
chemical_list: D011933:Reagent Kits, Diagnostic; D017971:Tin Compounds; C023599:stannous chloride; D013667:Technetium; D014001:Tin; D004492:Edetic Acid