[Isolation and characterization of erythrogenic toxins. VII. Study of the erythrogenic toxin type C produced by Streptococcus pyogenes].
Erythrogenic toxin (ET) type C was purified from culture filtrates of Streptococcus pyogenes strains NY 5, T 18, and AT 13. Methods used included ammonium sulfate precipitation, phosphate precipitation, column chromatography on CM-Sepharose and Sephadex G 100, and isoelectric focusing in Sephadex gel. The molecular weight was determined by SDS gel electrophoresis as 25,500. The preparation reacted only with homologous antiserum (anti-C), but not with antitoxins types A or B in double diffusion tests. The isoelectric point was determined to be 6.8 by analytical isoelectric focusing. Also the amino acid composition was determined. The toxin was found to be mitogenic (as well as pyrogenic and toxic for rabbits). The ET type C is digested by trypsin, pepsin, chymotrypsin and Pronase E, but is rather stable when treated with papain or streptococcal proteinase.
D000596:Amino Acids / Q000032:analysis; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000032:analysis / Q000302:isolation & purification*; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D004355:Drug Stability; D005098:Exotoxins; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D008565:Membrane Proteins; D008970:Molecular Weight; D013297:Streptococcus pyogenes / Q000032:analysis*
D000596:Amino Acids; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D005098:Exotoxins; D008565:Membrane Proteins; C117980:SpeA protein, Streptococcus pyogenes; C027951:erythrogenic toxin
Isolierung und Charakterisierung von erythrogenen Toxinen. VII. Untersuchung des von Streptococcus pyogenes gebildeten erythrogenen Toxins Typ C.