Properties of TAS-1D3, a tuberculin-active substance from BCG, in regard to delayed hypersensitivity.
The properties of TAS-1D3, a tuberculin-active substance purified from the cell extract of Mycobacterium bovis BCG, were studied in vivo and in vitro. In the delayed hypersensitivity skin reaction, TAS-1D3 showed far more potent activity than tuberculin purified protein derivative (PPD) in guinea pigs sensitized with BCG vaccine. This was consistently observed from 6 to 24 weeks after sensitization. The histological findings of the skin reaction to TAS-1D3 were similar to those of the reaction to PPD. Moreover, TAS-1D3 induced well both thymidine incorporation and the production of migration inhibitory factor (MIF) by the spleen cells from guinea pigs sensitized with BCG vaccine. In contrast, TAS-1D3 showed weaker activity than PPD in guinea pigs sensitized with either heat-killed M. tuberculosis Aoyama B or heat-killed M. tuberculosis H37Ra, and it weakly stimulated the spleen cells from animals sensitized with M. tuberculosis Aoyama B to incorporate thymidine and to produce MIF.
D000818:Animals; D001500:BCG Vaccine / Q000276:immunology; D001426:Bacterial Proteins; D003429:Cross Reactions; D005260:Female; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed; D066298:In Vitro Techniques; D009161:Mycobacterium / Q000276:immunology; D009163:Mycobacterium bovis / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology; D010455:Peptides / Q000276:immunology*; D012867:Skin / Q000276:immunology; D013154:Spleen / Q000276:immunology; D014373:Tuberculin / Q000276:immunology*; D014374:Tuberculin Test
D001500:BCG Vaccine; D001426:Bacterial Proteins; D010455:Peptides; D014373:Tuberculin; C009610:tuberculin-active protein, Mycobacterium