[Enzymatic conversion of L-histidine to urocanic acid using immobilized histidase from the rat liver].
A procedure is described for immobilization of partially purified histidase by means of covalent binding of the enzyme with amino ethyl cellulose activated by glutaraldehyde. The preparation obtained was stable within 6 months at 5 degrees and within 40 days at 39 degrees. The preparation of immobilized histidase might be used for enzymatic synthesis of urocanic acid from histidine.
D000180:Acrylic Resins; D000642:Ammonia-Lyases / Q000378:metabolism*; D000818:Animals; D002482:Cellulose / Q000031:analogs & derivatives; D004800:Enzymes, Immobilized / Q000378:metabolism; D005260:Female; D005976:Glutaral; D006639:Histidine / Q000378:metabolism*; D006638:Histidine Ammonia-Lyase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism*; D051381:Rats; D013696:Temperature; D014560:Urocanic Acid / Q000096:biosynthesis*
D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D007093:Imidazoles; D006639:Histidine; C032056:enzacryl AH; D002482:Cellulose; C010021:aminoethyl cellulose; D000642:Ammonia-Lyases; D006638:Histidine Ammonia-Lyase; D014560:Urocanic Acid; D005976:Glutaral
Fermentativnoe prevrashchenie L-gistidina v urokaninovuiu kisloty s pomoshch'iu immobilizovannoĭ gistidazy iz pecheni krysy.