title: Threonyl-tRNA synthetase from Chinese hamster ovary cells is phosphorylated on serine.
abstract: A Chinese hamster ovary cell line, 2000A, in which threonyl-tRNA synthetase accounts for 1.5% of the total soluble protein, was used to demonstrate that this enzyme is a phosphoprotein. Threonyl-tRNA synthetase was isolated by immunoprecipitation from cells labeled with 32Pi for 18 h. Phosphoamino acid analysis of radiolabeled threonyl-tRNA synthetase showed that phosphorylation occurs on serine.
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D010053:Ovary / Q000201:enzymology*; D010766:Phosphorylation; D012694:Serine / Q000378:metabolism*; D013914:Threonine-tRNA Ligase / Q000378:metabolism*
chemical_list: D012694:Serine; D000604:Amino Acyl-tRNA Synthetases; D013914:Threonine-tRNA Ligase