A new sensitive fluorometric method for measurement of vascular permeability.
A sensitive fluorometric method has been developed for the measurement of vascular permeability in carrageenin air-pouch inflammation in rats. Fluorescein isothiocyanate-labeled bovine serum albumin (F-BSA) was used as a tracer. This fluorometric method is as simple and reliable as the method using radioiodine-labeled human serum albumin and has the advantages of low cost, no health hazard, and the fact that F-BSA can be stored over a long period. This fluorometric method is probably applicable to other inflammation models such as pleurisy and peritonitis in which inflammatory exudate can be collected.
D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D002850:Chromatography, Gel; D003907:Dexamethasone / Q000494:pharmacology; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D007213:Indomethacin / Q000494:pharmacology; D007249:Inflammation / Q000503:physiopathology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012710:Serum Albumin, Bovine; D012711:Serum Albumin, Radio-Iodinated; D013050:Spectrometry, Fluorescence / Q000379:methods; D013861:Thiocyanates
D005452:Fluoresceins; D012711:Serum Albumin, Radio-Iodinated; D013861:Thiocyanates; D012710:Serum Albumin, Bovine; D003907:Dexamethasone; D016650:Fluorescein-5-isothiocyanate; D007213:Indomethacin