Choosing the right intravesical chemotherapeutic agent. Results of an in vitro monolayer cell culture assay.
The use of a monolayer cell culture technique is described. Twenty-four superficial bladder tumours have been tested against Thiotepa, Adriamycin and Mitomycin C. Cultures were set up in parallel for a clonogenic assay after the method of Salmon. In the monolayer all 24 tumours grew; 21 showed a greater than 50% inhibition to Mitomycin, 13 to Adriamycin and 6 to Thiotepa. In the clonogenic assays, only 12 grew satisfactorily. It is concluded that this monolayer assay gives a reasonable prediction of the sensitivities of the commonly used intravesical agents.
D000903:Antibiotics, Antineoplastic / Q000627:therapeutic use*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004317:Doxorubicin / Q000627:therapeutic use*; D006801:Humans; D016685:Mitomycin; D008937:Mitomycins / Q000627:therapeutic use*; D013852:Thiotepa / Q000627:therapeutic use*; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy*
D000903:Antibiotics, Antineoplastic; D008937:Mitomycins; D016685:Mitomycin; D004317:Doxorubicin; D013852:Thiotepa