[Conditions for immobilizing protosubtilin on aluminum oxide using transitional metal ions].
The effect of the degree of the carrier activation by titanium tetrachloride, type of buffer solution, and protein concentration on characteristics of protosubtiline G10x immobilized on alumina was studied. X-ray phase analysis and IR spectroscopy were used to investigate the structure of the carrier and its changes during activation. To obtain the immobilized enzyme preparation with the maximum activity, immobilization should be carried out in 0.02 M Ca-acetate buffer on alumina treated with 2% solution of titanium tetrachloride.
D000535:Aluminum / Q000494:pharmacology*; D000537:Aluminum Oxide / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000201:enzymology; D002021:Buffers; D004792:Enzyme Precursors / Q000032:analysis / Q000494:pharmacology*; D004800:Enzymes, Immobilized / Q000032:analysis / Q000494:pharmacology*; D007477:Ions; D008722:Methods; D010447:Peptide Hydrolases / Q000032:analysis / Q000494:pharmacology*; D013055:Spectrophotometry, Infrared; D014025:Titanium / Q000494:pharmacology; D014961:X-Ray Diffraction
D002021:Buffers; D004792:Enzyme Precursors; D004800:Enzymes, Immobilized; D007477:Ions; C025096:titanium tetrachloride; C040620:protosubtilin; D000535:Aluminum; D014025:Titanium; D010447:Peptide Hydrolases; D000537:Aluminum Oxide
Issledovanie usloviĭ immobilizatsii protosubtilina na okisi aliuminiia pri pomoshchi ionov perekhodnykh metallov.