Analysis and in vivo assay of B. abortus agglutinating and non-agglutinating antibodies.
Studies were made of physicochemical and immunochemical characteristics of Brucella abortus agglutinating and non-agglutinating antibodies in the sera of cattle repeatedly injected with living B. abortus (Strain 1119). Both agglutinating and non-agglutinating antibody were shown to be IgG1, and by immunodiffusion against rabbit anti-cattle gamma-globulin, agglutinating antibody gave a precipitation line of identity with that given by non-agglutinating antibody. Whilst agglutinating antibody increased clearance of antigen from the blood of passively protected mice, non-agglutinating antibody did not enhance clearance. Determination of the spleen infection index in mice pre-treated with agglutinating and non-agglutinating antibody showed that in animals passively immunized with non-agglutinating antibody the number of living (infecting) bacteria was approximately 4 times higher than in the case of agglutinating antibody. The possible potentiation of chronic B. abortus infection by non-agglutinating antibody is discussed.
D000371:Agglutination; D000373:Agglutinins / Q000032:analysis / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D002003:Brucella abortus / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000276:immunology; D002417:Cattle / Q000276:immunology*; D003298:Coombs Test; D005779:Immunodiffusion / Q000662:veterinary; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D008297:Male; D051379:Mice; D011817:Rabbits
D000373:Agglutinins; D000907:Antibodies, Bacterial; D007074:Immunoglobulin G