title: Occurrence of oxygen-sensitive, NADP+-dependent pyruvate dehydrogenase in mitochondria of Euglena gracilis.
abstract: Pyruvate dehydrogenase found in mitochondria of Euglena gracilis was active on NADP+ but not NAD+, and FAD and methyl viologen also served as electron acceptors. For 2-oxoglutarate dehydrogenase both NAD+ and NADP+ were utilized and the ratio of its activity on NAD+ and NADP+ was about 1:5. The activity of pyruvate dehydrogenase was inhibited by pyruvate in aerobiosis, while not in anaerobiosis.
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D004579:Electron Transport; D005056:Euglena gracilis / Q000201:enzymology*; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology*; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*
chemical_list: D011768:Pyruvate Dehydrogenase Complex; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D009249:NADP