title: Role of calcium ions in insulin action on hexose transport in L6 muscle cells.
abstract: It has been proposed that Ca2+ ions mediate the stimulation by insulin of glucose uptake in muscle (Clausen, T., Cell Calcium 1:311-325, 1980). However, absolute measurements of the concentration of cytosolic free Ca2+, [Ca2+]i, during the course of insulin action have not been made. The stimulation of hexose uptake by insulin was studied in an in vitro model system of muscle cells, the L6 cell line. The following evidence suggests that Ca2+ ions are not likely to fulfill the purported role. 1) Insulin in Ca2+-free media induced stimulation of 2-deoxy-D-glucose uptake. 2) Elevation of [Ca2+]i with the ionophore A23187 did not enhance hexose uptake. 3) Insulin action was not diminished when the hormone was added to Ca2+-depleted cells in Ca2+-free media with A23187. 4) Hexose uptake was not affected by a number of agents thought to modify [Ca2+]i including epinephrine, caffeine, 2,4-dinitrophenol, hyperosmolar mannitol, salicylate, vanadate, veratrine, and trypsin. 5) Direct determinations of [Ca2+]i by fluorescence of the novel indicator Quin-2 did not show differences between basal and insulin-stimulated cells; under identical conditions hexose uptake was stimulated by the hormone. 6) Chelation of [Ca2+]i with Quin-2 in Ca2+-free media did not affect the response to insulin. 7) Low concentrations of trypsin (7.5 micrograms/ml) elevated [Ca2+]i but did not increase the rate of hexose uptake.
mesh_terms: D000634:Aminoquinolines / Q000494:pharmacology; D000818:Animals; D001693:Biological Transport, Active; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000032:analysis / Q000502:physiology*; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D003600:Cytosol / Q000378:metabolism; D003847:Deoxyglucose / Q000378:metabolism; D005456:Fluorescent Dyes; D006601:Hexoses / Q000378:metabolism*; D007328:Insulin / Q000502:physiology*; D009132:Muscles / Q000166:cytology* / Q000378:metabolism; D051381:Rats
chemical_list: D000634:Aminoquinolines; D003470:Culture Media; D005456:Fluorescent Dyes; D006601:Hexoses; D007328:Insulin; D000001:Calcimycin; C035738:Quin2-acetoxymethyl ester; D003847:Deoxyglucose; D002118:Calcium