Determination of copper concentration in blood plasma and in ocular and cerebrospinal fluids using graphite furnace atomic absorption spectroscopy.
Published values for the concentration of Cu in cerebrospinal and intraocular fluids cover a very wide range (0.016 to 1.0 microgram/ml) and include values which are several times higher than those which would be consistent with normal physiology. An atomic absorption spectrophotometer equipped with a graphite furnace was used to measure the Cu concentration in these fluids and in blood plasma of toads, rabbits, and cats. Under standard conditions, these fluids yielded high background absorbance and only fractional recovery of added Cu. Parameters were therefore established which eliminated both the high background and the matrix interference and allowed the determination of Cu in 10-microliters aliquots of diluted blood plasma and undiluted cerebrospinal and ocular fluid samples. Under these conditions the Cu measured in the ocular (0.011 to 0.032 microgram/ml) and cerebrospinal fluids (0.033 to 0.050 microgram/ml) of these three species was lower than most previously reported values and only a small fraction (1-3%) of the concentration of Cu in the plasma of the same animals (0.85 to 1.22 micrograms/ml).
D000818:Animals; D001082:Aqueous Humor / Q000032:analysis*; D002024:Bufo marinus; D002415:Cats; D003300:Copper / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D005260:Female; D006108:Graphite; D008297:Male; D011817:Rabbits; D013045:Species Specificity; D013054:Spectrophotometry, Atomic / Q000379:methods; D014822:Vitreous Body / Q000032:analysis*
D006108:Graphite; D003300:Copper