title: Rapid and selective derivatization method for the nitrogen-sensitive detection of carboxylic acids in biological fluids prior to gas chromatographic analysis.
abstract: A rapid and selective derivatization procedure is described for the pre-column labelling of carboxylic acids with a nitrogen-containing label. The carboxylic acid function is activated with 2-bromo-1-methylpyridinium iodide and the activated carboxylic acid function reacts with a primary or a secondary amine to yield an amide. With flurbiprofen as the test compound and dipropylamine as a label the acid was completely converted to the corresponding amide. The method was tested for several aliphatic, aromatic and for phenylacetic or phenylpropionic carboxylic acid derivatives, and was found to result in the complete derivatization of these compounds with a few exceptions only. The derivatization procedure is potentially useful for drug monitoring purposes, as is shown with the analysis of valproic acid and flurbiprofen in plasma.
mesh_terms: D001826:Body Fluids / Q000032:analysis*; D002264:Carboxylic Acids / Q000032:analysis* / Q000097:blood; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D005480:Flurbiprofen / Q000097:blood; D006801:Humans; D012997:Solvents; D014635:Valproic Acid / Q000097:blood
chemical_list: D002264:Carboxylic Acids; D012997:Solvents; D005480:Flurbiprofen; D014635:Valproic Acid