Partial characterization of the putative rat interleukin 2 receptor.
T lymphoblasts of rat origin were (a) surface labeled with 125I and (b) internally labeled with 3H-marked sugars. Cell lysates were purified by immunoabsorption using the putative anti-rat interleukin 2 (IL2) receptor monoclonal antibody ART18 . The purified material was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoretic analysis. Two specific membrane components were detected: a 50-kDa major and a 36-kDa minor component in reducing and a 45-kDA major and a 72-kDa minor component in nonreducing conditions, respectively. Both components were found to be susceptible to trypsinization and to neuraminidase treatment. 3H-labeled sugars were incorporated into the major component. The results indicate that the rat IL2 receptor is either a 50-kDa glycoprotein or a 36-kDa molecule, or that both components are part of the receptor molecule.
D000818:Animals; D000911:Antibodies, Monoclonal; D004591:Electrophoresis, Polyacrylamide Gel; D007457:Iodine Radioisotopes; D009439:Neuraminidase / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew; D011971:Receptors, Immunologic / Q000302:isolation & purification*; D015375:Receptors, Interleukin-2; D012967:Sodium Dodecyl Sulfate; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014316:Tritium; D014357:Trypsin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D014316:Tritium; D012967:Sodium Dodecyl Sulfate; D009439:Neuraminidase; D014357:Trypsin