title: The effect of solubilized bone matrix fractions from different mammalian species on glycosaminoglycan synthesis by cultured fibroblasts.
abstract: Human and bovine bone matrices were extracted with salt solutions of different composition and the extracts tested for stimulation of incorporation of radioactivity from [3H]glucosamine and [35S]SO4 into the hyaluronic acid and chondroitin sulfate of the cell pellet, the cell surface and the medium fractions of human synovial cells in culture. Stimulatory activity was extracted with a solution of 0.3 M EDTA in 2.5 M NaCl from bovine but not human bone. Subsequent extraction of the residues with 4 M guanidinium hydrochloride yielded activity from both matrices. A major stimulation of incorporation of radioactivity was observed in the cell surface fractions. Human synovial cells constitute a more sensitive assay system for the stimulatory activity than rabbit synovial cells.
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D002417:Cattle; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis; D007700:Kinetics; D051381:Rats; D012995:Solubility; D013045:Species Specificity; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes; D013583:Synovial Membrane / Q000378:metabolism*; D014020:Tissue Extracts / Q000494:pharmacology*; D014316:Tritium
chemical_list: D006025:Glycosaminoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes; D014020:Tissue Extracts; D014316:Tritium; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D005944:Glucosamine