title: Blockade of the estrogen-induced luteinizing hormone surge in monkeys: a nonsteroidal, antigenic factor in porcine follicular fluid.
abstract: That both follicular fluid extracts and ovarian hyperstimulation block estrogen-induced gonadotropin surges in primates has been established. In the present study, the differential actions of gonadotropin-releasing hormone (GnRH) and estradiol benzoate (E2B) on bioassayable LH (BIO-LH) secretion in the presence of a porcine follicular fluid (pFF) extract were assessed. Further, the inherent immunogenicity of the follicular fluid factor(s) that block estrogen-induced surges was examined. In castrated monkeys, administration of Florisil/charcoal-extracted pFF did not affect BIO-LH secretion induced by exogenous GnRH, although the pFF extract abolished E2B-induced BIO-LH surges. In monkeys given pFF chronically (for 6 to 8 months), immune responses capable of neutralizing the inhibitory actions of pFF on the E2B-induced BIO-LH surges developed. In these pFF-immunized monkeys, the BIO-LH surge induced by an E2B challenge was restored despite concurrent pFF injections.
mesh_terms: D000818:Animals; D002940:Circadian Rhythm; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D005779:Immunodiffusion; D007986:Luteinizing Hormone / Q000097:blood*; D008253:Macaca mulatta; D008297:Male; D006080:Ovarian Follicle / Q000378:metabolism*; D013552:Swine; D014020:Tissue Extracts / Q000494:pharmacology*
chemical_list: D014020:Tissue Extracts; D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol; D007986:Luteinizing Hormone