Relationship between the anti-phospholipase and anti-inflammatory effects of glucocorticoid-induced proteins.
Glucocorticoid-induced anti-phospholipase proteins were partially purified by using ion-exchange and molecular sieve chromatography. These proteins, as well as dexamethasone itself, inhibited the hind-paw rat oedema induced by carrageenin. This inhibition was reversed by arachidonic acid, Anti-phospholipase proteins as well as hydrocortisone, also reduced the formation of prostaglandin E2 and leukotriene B4 by phagocytosing leucocytes. A specific monoclonal antibody was able to reverse the inhibition of eicosanoid formation. The mechanism of the anti-inflammatory effect of glucocorticoids and anti-phospholipase proteins is discussed in the light of these results.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D002351:Carrageenan; D002852:Chromatography, Ion Exchange; D003907:Dexamethasone / Q000494:pharmacology; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005938:Glucocorticoids / Q000494:pharmacology*; D006854:Hydrocortisone / Q000494:pharmacology; D007962:Leukocytes / Q000187:drug effects; D008297:Male; D010740:Phospholipases / Q000037:antagonists & inhibitors*; D014176:Protein Biosynthesis; D011506:Proteins / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D000893:Anti-Inflammatory Agents; D001095:Arachidonic Acids; D005938:Glucocorticoids; D011506:Proteins; D016718:Arachidonic Acid; D003907:Dexamethasone; D002351:Carrageenan; D010740:Phospholipases; D006854:Hydrocortisone