Presence of B-100 in rat mesenteric chyle.
Molecular forms of apolipoprotein B (ApoB) were studied in the rat intestinal chyle by SDS-polyacrylamide gel electrophoresis, immunoblotting and immunodiffusion. Time studies on intestinal chyle showed the presence of B-100 in all the samples analyzed within 3 hr after drawing. However, the analyses repeated on day 2 or day 3 revealed disappearance of B-100 and appearance of B-48. Addition of 3 mM EDTA, 10 mM diisopropylfluorophosphate, 5 mM chloroquine and 10 mM epsilon-amino caproic acid slowed down but could not prevent the disappearance of B-100. Chylomicrons isolated from chyle in the presence of preservatives immediately after drawing displayed B-100 as a major and B-48 as a minor ApoB form. However, repeatedly washed chylomicrons or those isolated from chyle 18-24 hr after drawing showed B-48 as the only ApoB present. These results suggest that rat intestine synthesizes B-100 which is quickly converted to smaller molecular form.
D000818:Animals; D001055:Apolipoproteins B / Q000032:analysis*; D002913:Chyle / Q000032:analysis*; D002914:Chylomicrons / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D008077:Lipoproteins, LDL / Q000302:isolation & purification; D008196:Lymph / Q000032:analysis; D008297:Male; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains
D001055:Apolipoproteins B; D002914:Chylomicrons; D008077:Lipoproteins, LDL