Interaction of a streptococcal cell wall tetraheteroglycan and Salmonella telaviv lipopolysaccharide with polymeric McPC 870 and MOPC 384 mouse IgA myeloma proteins.
Interactions of polymeric McPC 870 and MOPC 384 mouse IgA myeloma proteins with a streptococcal cell wall tetraheteroglycan (T4-antigen), Salmonella tranoroa lipopolysaccharide (LPS), S. telaviv LPS and its lipid A free polysaccharide were examined by the quantitative precipitin method. Hapten inhibition of myeloma protein precipitation by T4-antigen or S. telaviv LPS indicates a complex anticarbohydrate specificity for 870 and 384 protein combining sites involving several sugars. While the combining site of 870 protein appears to bind alpha-D-mannopyranosyl, alpha-D-galactopyranosyl and alpha-D-glucopyranosyl residues, the combining site of 384 protein can accommodate both the alpha (1----2)-linked glucobiose (kojibiose) and alpha-D-galactopyranosyl but not the alpha-D-mannopyranosyl structure.
D000818:Animals; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D001666:Binding Sites, Antibody; D002473:Cell Wall / Q000276:immunology; D002850:Chromatography, Gel; D006241:Haptens / Q000276:immunology; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A / Q000276:immunology; D008050:Lipid A; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D009194:Myeloma Proteins / Q000276:immunology*; D011233:Precipitin Tests; D012475:Salmonella / Q000276:immunology*; D013291:Streptococcus / Q000276:immunology*
D000942:Antigens, Bacterial; D006241:Haptens; D007070:Immunoglobulin A; D008050:Lipid A; D008070:Lipopolysaccharides; D009194:Myeloma Proteins