Ca binding of isolated cardiac nexus membranes related to intercellular uncoupling.
Nexus membranes were isolated from cardiac muscles of guinea pig or ox. It was confirmed by autoradiography that Ca2+ was actually bound with the nexus, and by atomic absorption spectroscopy that bound Ca increased extensively at [Ca2+] higher than 10(-6) to 10(-5) M and showed the dose-response relationship against [Ca2+].
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008274:Magnesium / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*
D000251:Adenosine Triphosphatases; D008274:Magnesium; D002118:Calcium