Influence of messenger RNA secondary structure on translation efficiency.
Two genes for human immune interferon (hIFN-gamma), synthetic (GIFs) and cDNA prepared from mRNA (GIFm), have been expressed in E. coli under the control of the trp promoter. The expression level of GIFs was 100-fold lower than that of GIFm. Secondary structure analysis of two genes' mRNA surrounding Shine-Dalgarno (SD) sequence showed the presence of a 8 base-pair stable stem structure preceding SD sequence in GIFs mRNA, but not in GIFm mRNA. Expression of a GIFs gene, in which the stem structure was rendered unstable by changing two bases, became 100 times greater than that of the original GIFs.
D001483:Base Sequence; D002678:Chimera; D004247:DNA / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005813:Genes, Synthetic; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D009690:Nucleic Acid Conformation; D009876:Operon; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*
D012333:RNA, Messenger; D007371:Interferon-gamma; D004247:DNA