Apparatus and method for preparative gel electrophoresis.
A new apparatus for preparative gel electrophoresis with continuous elution which includes a miniaturized electrode and elution chamber system is described. The design provides high resolution, high yield, applicability for small and large amounts of peptide material, and easy operation. Furthermore, the apparatus enables a very accurate gel column or gel gradient to be formed. A method for preparative gel electrophoresis in sodium dodecyl sulfate which allows the purification of peptides and proteins without concurrently modifying tryptophane residues or blocking N-terminal alpha-amino groups is also described.
D000595:Amino Acid Sequence; D000818:Animals; D001322:Autoanalysis; D001412:Bacillus subtilis / Q000201:enzymology; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D000458:Cyanobacteria / Q000032:analysis; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation* / Q000379:methods; D006736:Horses; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification; D009211:Myoglobin / Q000302:isolation & purification; D010798:Phycocyanin / Q000302:isolation & purification; D014364:Tryptophan / Q000302:isolation & purification
D009211:Myoglobin; D010798:Phycocyanin; D014364:Tryptophan; D003577:Cytochrome P-450 Enzyme System; D007770:L-Lactate Dehydrogenase