Enhancement of Leptospira hardjo agglutination titers in sheep and goat serum by heat inactivation.
Heat inactivation of sheep serum samples resulted in the detection of an additional 9% reactors to Leptospira hardjo that were negative on the initial test of fresh samples. Treatment with EDTA gave results generally similar to heat inactivation suggesting that complement was responsible for the inhibition of agglutination. Tests on heat inactivated serum from experimentally infected sheep and goats revealed enhanced titers or reactions which were not detected in fresh serum.
D000372:Agglutination Tests / Q000662:veterinary*; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D003165:Complement System Proteins / Q000276:immunology; D004492:Edetic Acid / Q000494:pharmacology; D005188:False Negative Reactions; D006041:Goats / Q000276:immunology*; D006358:Hot Temperature; D007919:Leptospira / Q000276:immunology*; D012756:Sheep / Q000276:immunology*
D000907:Antibodies, Bacterial; D003165:Complement System Proteins; D004492:Edetic Acid