title: Reconstituted collagen fibrils. Fibrillar and molecular stability of the collagen upon maturation in vitro.
abstract: During the maturation in vitro of reconstituted collagen fibrils prepared from rat skin, the mechanical and thermal stability of collagen increased and the pepsin-solubility decreased. At the same time a larger fraction of the pepsin-soluble collagen attained a lower molecular thermal stability that resulted in a biphasic thermal transition of the soluble collagen. Type-I collagen, with a similar biphasic thermal transition, was isolated from acid-insoluble rat skin collagen.
mesh_terms: D000818:Animals; D003094:Collagen / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D046911:Macromolecular Substances; D008297:Male; D010434:Pepsin A; D011489:Protein Denaturation; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000378:metabolism*; D013696:Temperature
chemical_list: D046911:Macromolecular Substances; D003094:Collagen; D010434:Pepsin A