Immunologic evaluation of T chronic lymphocyte leukaemia cells in a patient with a concomitant IgA2 myeloma.
Peripheral blood lymphocytes from a patient with OKT3, OKT4, OKT17 positive T CLL and a concomitant IgA2/lambda myeloma were found to be suppressive in cocultivation experiments and after in vitro preactivation . Although chromosomal analysis after stimulation with T or B cell mitogens suggested a common origin of the two malignancies, T cells did not carry structures recognized by an anti-idiotypic antiserum raised against the purified M-component. Only Fc mu receptors were found on the T cells with conventional rosetting assays, although a weak binding of Latex particles coated with the purified IgA M-component could occasionally be recorded. When purified T cells were submitted to FACS-analysis after incubation with the FITC-conjugated purified M-component, two subsets could easily be distinguished in normal T cells, whereas only the high affinity-binding subset could be found in the patient.
D000368:Aged; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D007070:Immunoglobulin A; D007146:Immunoglobulin lambda-Chains; D007621:Karyotyping; D007945:Leukemia, Lymphoid / Q000150:complications*; D008213:Lymphocyte Activation; D009101:Multiple Myeloma / Q000150:complications*; D011947:Receptors, Antigen, B-Cell / Q000276:immunology; D011961:Receptors, Fc / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification / Q000166:cytology / Q000276:immunology*
D007070:Immunoglobulin A; D007146:Immunoglobulin lambda-Chains; D011947:Receptors, Antigen, B-Cell; D011961:Receptors, Fc