Sister-chromatid exchange and micronucleus induction as indicators of genetic damage in maternal and foetal cells.
The effectiveness of 3 compounds, procarbazine, mitomycin C and cyclophosphamide as inducers of sister-chromatid exchanges (SCEs) in granulocyte-macrophage progenitor cells, in foetal liver and bone marrow from pregnant mice at day 17 of gestation were determined. Cyclophosphamide and procarbazine induced similar SCE frequencies in maternal and foetal cells. Mitomycin C was slightly less effective in foetal liver than in maternal bone marrow. In contrast to the results of SCE induction, cyclophosphamide produced more micronucleated polychromatic erythrocytes in foetal liver than in bone marrow. The SCE results for mitomycin C and procarbazine are compared with results obtained previously for micronuclei induction in 15-day pregnant animals.
D000818:Animals; D001854:Bone Marrow Cells; D002467:Cell Nucleus / Q000648:ultrastructure*; D003434:Crossing Over, Genetic; D003520:Cyclophosphamide / Q000494:pharmacology; D005260:Female; D008099:Liver / Q000196:embryology; D051379:Mice; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009152:Mutagenicity Tests; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000187:drug effects*; D011344:Procarbazine / Q000494:pharmacology; D012854:Sister Chromatid Exchange
D008937:Mitomycins; D011344:Procarbazine; D016685:Mitomycin; D003520:Cyclophosphamide