Activation of bovine platelets induced by long-chain unsaturated fatty acids at just below their lytic concentrations, and its mechanism.
The effects of long-chain unsaturated fatty acids such as linoleic acid on bovine platelets were examined. Not only linoleic acid, but also oleic and linolenic acid, at just below the concentrations causing marked cell lysis, induced an absorbance decrease of the platelet suspension in the presence of Ca2+. Since this absorbance decrease was reversed by the addition of EDTA and moreover aggregate formation was found by macroscopic and microscopic observation, it was concluded that unsaturated fatty acids at just below their lytic concentrations caused platelet aggregation. Unsaturated fatty acids also caused release of adenine nucleotides, but there was a lag time between the release and the aggregation, just as with ADP-induced release, suggesting that the aggregation was independent of the release of ADP. It was revealed that this activation of platelets by unsaturated fatty acids was caused by marked Ca2+ uptake into the cytoplasm, resulting from significant membrane perturbation.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D005576:Colforsin; D004224:Diterpenes / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005454:Fluorescence Polarization; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D008560:Membrane Fluidity / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D013748:Tetracaine / Q000494:pharmacology
D004224:Diterpenes; D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D013748:Tetracaine; D005576:Colforsin; D000255:Adenosine Triphosphate; D004492:Edetic Acid; D019787:Linoleic Acid; D002118:Calcium