title: Chemical mutagenesis testing in Drosophila: I. Comparison of positive and negative control data for sex-linked recessive lethal mutations and reciprocal translocations in three laboratories.
abstract: As part of the validation phase of the Drosophila melanogaster segment of the National Toxicology Program, a comparison has been made of positive and negative controls for sex-linked recessive lethal mutations and reciprocal translocations from three laboratories. This comparison involves approximately 700,000 spontaneous recessive lethal mutation tests, 70,000 spontaneous translocation tests, and screens for genetic damage induced by N-nitrosodimethylamine and beta-propiolactone. Spontaneous frequencies for lethal mutations and translocations were homogeneous in the laboratories regardless of solvent or broods sampled. Inhomogeneity was observed in induced frequencies among laboratories, but the variation was no greater than that found within a laboratory.
mesh_terms: D000818:Animals; D004128:Dimethylnitrosamine / Q000633:toxicity; D004331:Drosophila melanogaster / Q000235:genetics*; D005787:Gene Frequency; D005804:Genes, Lethal; D005808:Genes, Recessive; D009096:Multi-Institutional Systems; D009152:Mutagenicity Tests; D009153:Mutagens; D009154:Mutation; D011420:Propiolactone / Q000633:toxicity; D012016:Reference Values; D012730:Sex Chromosomes; D014178:Translocation, Genetic
chemical_list: D009153:Mutagens; D011420:Propiolactone; D004128:Dimethylnitrosamine