Secretion of a macrophage-activating factor distinct from interferon-gamma by human T cell clones.
Supernatants from clones of human T lymphocytes that were responding to a purified Mycobacterium tuberculosis antigen were able to activate macrophages and macrophage-like myeloma cells (U937) to release increased amounts of the microbicidal agent hydrogen peroxide. The activity was not neutralized by monoclonal antibody against interferon-gamma (IFN-gamma), was greater than could be accounted for by the IFN-gamma activity in the supernatants, and was separated from IFN-gamma by high performance liquid chromatography. It is evident that IFN-gamma is not the only macrophage activator released by T lymphocytes responding to microbial antigen, and may not even be the main one to enhance antimicrobial activity in infections such as tuberculosis.
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D002999:Clone Cells; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D008222:Lymphokines / Q000378:metabolism*; D016215:Macrophage-Activating Factors; D009169:Mycobacterium tuberculosis / Q000276:immunology; D013601:T-Lymphocytes / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D008222:Lymphokines; D016215:Macrophage-Activating Factors; D007371:Interferon-gamma