[Use of monoclonal antibodies against the light-chain immunoglobulins of the rat in the radioimmune analysis of differentiated lymphocyte antigens of the mouse].
Spleen cells from SJL mice immunized with rat immunoglobulin light chains were fused with X-63 myeloma. Seven hybridoma lines were obtained. Monoclonal antibodies (McAB) secreted by lines L1G9, L2B2 and L3E8 were purified and labeled by 125I. McAB L1G9 did not bind directly to murine lymphocytes but reacted in a different manner with rat splenocytes. McAb L2B2 and L3E8 but not L1G9 reacted with murine lymphocytes treated with rat McAb against Lyt-1, Lyt-2, C1-9, C4-20 antigens whatever the class of heavy chains of rat McAb used. The binding levels obtained with 125I-McAb reflect the quantitative differences in the expression of respective antigens.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000302:isolation & purification; D000954:Antigens, Surface / Q000032:analysis*; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007147:Immunoglobulin Light Chains / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000276:immunology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007147:Immunoglobulin Light Chains
Ispol'zovanie monoklonal'nykh antitel protiv legkikh tsepeĭ immunoglobulinov krysy v radioimmunnom analize differentsirovannykh antigenov limfotsitov myshi.