Protein-energy malnutrition alters theophylline effect on DNA synthesis in neonatal rat brain in vitro.
The rate of incorporation of [3H]-thymidine into DNA in whole brain in response to various concentrations of theophylline was studied in normal and malnourished neonatal rats in vitro. Malnutrition appeared to produce an increased sensitivity to theophylline. [3H]-Thymidine uptake was significantly increased in normally nourished animals at a concentration of 2 X 10(-4) mol/l (36 micrograms/ml), while malnourished animals showed a similar effect at 1 X 10(-4) mol/l (18 micrograms/ml). These data suggest that the nutritional status of a growing organ could be important determining the cellular effects of conventionally dosed theophylline under clinical conditions.
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D066298:In Vitro Techniques; D011247:Pregnancy; D011502:Protein-Energy Malnutrition / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013806:Theophylline / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
D004247:DNA; D013806:Theophylline; D013936:Thymidine