Allelic exclusion and control of endogenous immunoglobulin gene rearrangement in kappa transgenic mice.
Hybridomas were produced from spleen cells of kappa transgenic mice to investigate expression of the transgenic kappa gene, its effect on allelic exclusion and its effect on the control of light-chain gene rearrangement and expression. Our results show that the transgene is expressed normally and that the production of a complete immunoglobulin molecule turns off light-chain gene rearrangement.
D000483:Alleles; D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology; D001483:Base Sequence; D005786:Gene Expression Regulation; D005796:Genes; D005818:Genetic Engineering; D006825:Hybridomas; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D011995:Recombination, Genetic; D014158:Transcription, Genetic
D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains