Specific quantitation of plasma medroxyprogesterone acetate by gas chromatography/mass spectrometry.
Investigation of various derivatives (O-methyloxime, trifluoroenol acetate and t-butyldimethylsilyl enol ether) coupled with the efficient preparation of a trideuterated analogue of medroxyprogesterone acetate, has allowed the development of a rapid and accurate assay for its quantitation in plasma by isotope dilution gas chromatography/mass spectrometry. High oral doses (2.8 g weekly) of medroxyprogesterone acetate are shown to lead consistently to plasma levels of less than 16 ng.ml-1.
D002784:Cholesterol; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008525:Medroxyprogesterone / Q000031:analogs & derivatives* / Q000097:blood; D017258:Medroxyprogesterone Acetate
D002784:Cholesterol; D017258:Medroxyprogesterone Acetate; D008525:Medroxyprogesterone