[Purification of soybean trypsin inhibitor by an immunosorption method].
A technique for isolation of the trypsin inhibitor from soya beans (Kunitz inhibitor) was developed with affinity chromatography as a main step, the immobilized antibodies of the inhibitor being used as a sorbent. The inhibitor obtained was homogeneous according to the data of electrophoresis in PAAG and had the specific activity equal to that of an inhibitor preparation obtained by affinity chromatography on trypsin-sepharose.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007114:Immunization; D005779:Immunodiffusion; D007163:Immunosorbent Techniques; D008297:Male; D011817:Rabbits; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000276:immunology / Q000302:isolation & purification*; D014361:Trypsin Inhibitors / Q000302:isolation & purification*
D000906:Antibodies; D014361:Trypsin Inhibitors; D014360:Trypsin Inhibitor, Kunitz Soybean
Ochistka soevogo ingibitora tripsina metodom immunosorbtsii.