Murine gamma interferon activates the release of a macrophage-derived Ia-inducing factor that transfers Ia inductive capacity.
In this report we demonstrate that when the murine macrophage tumor cell line P- 388D1 is incubated for 48-72 h with either concanavalin A-stimulated rat spleen cell supernatant or cloned murine immune interferon (IFN-gamma), the cultured cells release a cell-free factor activity that in turn induces the cell surface expression of Ia antigen on the murine monocyte cell line WEHI-3. This IFN-gamma-stimulated, Ia-inducing activity cannot be blocked with an anti-IFN-gamma heteroantiserum that does block the induction of Ia expression on WEHI-3 by both cloned murine IFN-gamma and rat Con A supernatant. The Ia-inducing factor ( IaIF ) generated from P- 388D1 after stimulation by IFN-gamma does not demonstrate any antiviral activity. The P- 388D1 -derived IaIF is not shed plasma membrane Ia glycoprotein molecules, as demonstrated by the inability of the active component to bind specifically to an anti-I-Ad affinity column or to a protein A column after the active supernatant is first treated with an excess of anti-I-E/Cd,k monoclonal antibody.
D000818:Animals; D000906:Antibodies / Q000502:physiology; D000954:Antigens, Surface / Q000032:analysis; D000998:Antiviral Agents / Q000494:pharmacology; D001667:Binding, Competitive; D002460:Cell Line; D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D003208:Concanavalin A / Q000502:physiology; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000032:analysis* / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology / Q000502:physiology*; D008211:Lymphocyte Cooperation; D008222:Lymphokines / Q000096:biosynthesis* / Q000502:physiology; D008262:Macrophage Activation; D016215:Macrophage-Activating Factors; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D051381:Rats
D000906:Antibodies; D000954:Antigens, Surface; D000998:Antiviral Agents; D000949:Histocompatibility Antigens Class II; D008222:Lymphokines; D016215:Macrophage-Activating Factors; C021649:concanavalin A-induced helper factors; D003208:Concanavalin A; D007371:Interferon-gamma