The idiotypic characteristics of human antibodies to factor VIII.
We have prepared an antiidiotype antibody that specifically reacts with and inactivates a human autoantibody to factor VIII (VIII:C). The antiidiotype specificity of this rabbit antibody was evident in solution, as specific inhibition of anti-VIII:C activity, and in solid-phase studies. The antiidiotype antibody inhibited both plasma anti-VIII:C and Fab' fragments prepared from that plasma's IgG. Although the antibody had a greater inhibitory effect on the anti-VIII:C used for immunization than it did for other anti-VIII:C, partial inhibition was identified with ten of 25 other human anti-VIII:C. The demonstration that antiidiotype antibodies can be prepared to human anti-VIII:C indicates the feasibility of this approach to the immunochemical characterization of human anti-VIII:C inhibitors. Moreover, antiidiotype reagents have a potential role in the treatment of inhibitor patients.
D000042:Absorption; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification* / Q000502:physiology; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology*; D001667:Binding, Competitive; D005169:Factor VIII / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D011817:Rabbits; D014841:von Willebrand Factor
D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D014841:von Willebrand Factor; D005169:Factor VIII