Omega-hydroxylation of 12-L-hydroxy-5,8,10,14-eicosatetraenoic acid in human polymorphonuclear leukocytes.
Incubation of arachidonic acid or 12-Ls-hydroxy-5,8,10,14-eicosatetraenoic acid with suspensions of human polymorphonuclear leukocytes led to the formation of 12-Ls,20-dihydroxy-5,8,10,14-eicosatetraenoic acid. The structure of the new metabolite was by physical methods and by chemical degradation.
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood*; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006900:Hydroxylation; D009504:Neutrophils / Q000378:metabolism*; D010084:Oxidation-Reduction
D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D016718:Arachidonic Acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid