Protein-enhanced fluorescein chemiluminescence used in an immunoassay for rubella antibody in serum.
The chemiluminescence of fluorescein derivatives is greatly enhanced when they are conjugated to proteins. We exploited this property in devising a chemiluminescence immunoassay for rubella antibodies. In this sequential "sandwich"-type assay, tubes coated with rubella antigen are used for antibody separation and goat antibody to human IgG conjugated with fluorescein isothiocyanate is used for detection of bound rubella antibodies.
D000914:Antibodies, Viral / Q000032:analysis* / Q000592:standards; D002118:Calcium; D017610:Calcium Compounds; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006801:Humans; D008163:Luminescent Measurements; D011863:Radioimmunoassay; D012412:Rubella virus / Q000276:immunology*; D012973:Sodium Hypochlorite; D013861:Thiocyanates
D000914:Antibodies, Viral; D017610:Calcium Compounds; D005452:Fluoresceins; D013861:Thiocyanates; C004488:calcium hypochlorite; D012973:Sodium Hypochlorite; D016650:Fluorescein-5-isothiocyanate; D002118:Calcium