Isolation of a cDNA clone complementary to sequences for a 34-kilodalton protein which is a pp60v-src substrate.
We have isolated a partial cDNA clone containing sequences complementary to a mRNA encoding a 34- to 36-kilodalton normal chicken cell protein which is a substrate for pp60v-src kinase activity. Using this 34-kilodalton cDNA clone as a probe, we determined that the size of the 34-kilodalton mRNA was 1,100 nucleotides and the level of the 34-kilodalton RNA was the same in various tissues of mature chickens but was significantly higher in chicken embryo fibroblast cells.
D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D005347:Fibroblasts / Q000378:metabolism; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D015688:Oncogene Protein pp60(v-src); D011494:Protein Kinases / Q000378:metabolism*; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D013379:Substrate Specificity; D014764:Viral Proteins / Q000378:metabolism*
D011506:Proteins; D012333:RNA, Messenger; D014764:Viral Proteins; D004247:DNA; D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src)