The induction of Leu-1 antigen expression in human malignant and normal B cells by phorbol myristic acetate (PMA).
Malignant cells from five patients with B cell leukemia or lymphoma were cultured with phorbol myristic acetate (PMA). PMA was found to induce cell surface expression of the Leu-1 antigen in cells from three of the five patients. Using one- and two-color immunofluorescence staining and flow cytometry, we have shown simultaneous expression of the Leu-1 antigen with other B cell markers. Induction of Leu-1 antigen expression was not related to histologic subtype, in vitro secretion of immunoglobulin, or other clinical features. Biosynthetic labeling experiments showed that synthesis of Leu-1 antigen occurred and preceded expression of the antigen on the cell surface. PMA also induced the appearance of Leu-1 antigen-positive B cells in cultures of normal peripheral blood mononuclear cells. We propose that the Leu-1 antigen is expressed transiently during the differentiation of some B cells.
D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D007938:Leukemia / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008223:Lymphoma / Q000276:immunology; D010704:Phorbols / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D007136:Immunoglobulins; D010704:Phorbols; D013755:Tetradecanoylphorbol Acetate