Construction of expression plasmids producing high levels of human immuno interferon in E. coli.
Improved expression vectors have been constructed which are derived from runaway-replication mutants of plasmid pYM307 and carry the strong hybrid promoter tac with lac iq gene. The activity of this promoter is controlled by lac repressor, product of the lac iq gene. Heat induction leads to amplification of the plasmid copy number. This system was used for high level expression of the chemically synthesized gene for human immune interferon (hIFN-7). 3 h after induction at 37 degrees C the hIFN-7 amounted to about 20% of total cellular proteins.
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D006358:Hot Temperature; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D010957:Plasmids
D007371:Interferon-gamma