title: Different polysaccharides in the external layers (capsule and slime) of the cell envelope of Rhodopseudomonas capsulata Sp11.
abstract: Two different acidic polysaccharides (I and II) were detected in the external cell envelope layers (slime and capsule) of Rhodopseudomonas capsulata Sp11. Polysaccharide I contains rhamnose, fucose, glucosamine and an unknown acidic sugar, it represents the slime material of the strain. Polysaccharide II contains rhamnose, galactose, 3-amino-3,6-dideoxygalactose, an unknown amino sugar and galacturonic acid, it represents very likely the capsule of R. capsulata Sp11. Polysaccharide I has a serological specificity different from that of polysaccharide II as shown by immunoprecipitation using antisera against living cells. Polysaccharide II, but not polysaccharide I, reacts in antiserum against heat-treated cells (100 degrees C, 2.5 h). Whole cells are agglutinated in the antisera against living but not in those against heat-treated cells.
mesh_terms: D002462:Cell Membrane / Q000032:analysis; D002473:Cell Wall / Q000032:analysis; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D012241:Rhodopseudomonas / Q000032:analysis*; D014461:Ultracentrifugation
chemical_list: D011135:Polysaccharides, Bacterial