Combination therapy with misonidazole and mitomycin C: lack of chemosensitization of EMT6 tumor cells in vivo or in vitro.
The effect of the nitroimidazole misonidazole on the cytotoxicity of mitomycin C was examined using EMT6 Rw tumors in mice and EMT6 cells in culture. In contrast to reports by others using a different tumor system, we were unable to detect any significant increase of mitomycin cytotoxicity by misonidazole in vivo using tumor cell survival or host toxicity endpoints. Studies with cells in vitro provided no evidence for marked chemosensitization of either hypoxic or aerobic cells.
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000627:therapeutic use*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D008920:Misonidazole / Q000627:therapeutic use*; D016685:Mitomycin; D008937:Mitomycins / Q000627:therapeutic use*; D009368:Neoplasm Transplantation; D009593:Nitroimidazoles / Q000627:therapeutic use*; D010100:Oxygen / Q000502:physiology; D011838:Radiation-Sensitizing Agents / Q000627:therapeutic use; D013997:Time Factors
D000903:Antibiotics, Antineoplastic; D008937:Mitomycins; D009593:Nitroimidazoles; D011838:Radiation-Sensitizing Agents; D016685:Mitomycin; D008920:Misonidazole; D010100:Oxygen