Kinetics of induction and decay of error-prone DNA repair activity in Escherichia coli after treatment with nalidixic acid.
Inducible error-prone DNA repair activity was detected by infecting nalidixic acid-pretreated E. coli cells with UV-irradiated phage phi X174. Induction and decay kinetics of reactivation very much resembled that of mutagenesis of the UV-damaged phage. Repair as well as mutagenic activity increased for about 30 min. The maximal error-prone repair capacity, which was induced in the cell during the 30 min nalidixic acid treatment, rapidly died out during subsequent cell growth in absence of nalidixic acid. Induction of this repair mode was not observed in a recA- mutant. In the presence of nalidixic acid plus rifampicin both repair and mutagenic effects were abolished.
D010584:Bacteriophage phi X 174 / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects*; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000528:radiation effects; D004790:Enzyme Induction; D004926:Escherichia coli / Q000378:metabolism*; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology*; D012293:Rifampin / Q000494:pharmacology
D004269:DNA, Bacterial; D004279:DNA, Viral; D009268:Nalidixic Acid; D012293:Rifampin