Regulation of muscle phosphorylase kinase by actin and calmodulin.
The activation of muscle phosphorylase kinase b by actin has been studied. F-actin which is polymerized by 2 mM MgCl2 is a more effective activator of phosphorylase kinase than F-actin polymerized by 50 mM KCl. There is evidence suggesting that the activation of phosphorylase kinase by actin is not due to trace contamination of actin preparations with calmodulin: (1) Troponin I and trifluoperazine inhibit the activation of phosphorylase kinase by calmodulin but do not inhibit the activation of phosphorylase kinase by F-actin. (2) The activation induced by saturating concentrations of calmodulin and actin is additive both at pH 8.2 and at pH 6.8. (3) The activation of phosphorylase kinase by calmodulin and actin has different pH profiles. An addition of F-actin does not affect the apparent Km value for ATP but increases the sensitivity to phosphorylase b and the value of Vmax.
D000199:Actins / Q000502:physiology*; D000818:Animals; D002147:Calmodulin / Q000494:pharmacology*; D004347:Drug Interactions; D004789:Enzyme Activation; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D009132:Muscles / Q000201:enzymology*; D010764:Phosphorylase Kinase / Q000378:metabolism*; D010763:Phosphorylase b / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D011817:Rabbits; D014268:Trifluoperazine / Q000494:pharmacology; D014336:Troponin / Q000502:physiology; D019210:Troponin I
D000199:Actins; D002147:Calmodulin; D014336:Troponin; D019210:Troponin I; D014268:Trifluoperazine; D010763:Phosphorylase b; D010764:Phosphorylase Kinase; D008274:Magnesium; D011188:Potassium