[Development of early erythroid precursors (BFUe) from mouse bone marrow in a serum-free culture media. Effect of hemin].
In culture medium containing albumin, iron saturated transferrin, phospholipids, cholesterol and erythropoietin, BFUe growth requires foetal Calf serum. Without serum the BFUe development is advantageously restored by the addition of hemin associated with spleen conditioned medium. In erythropoietin supplemented serum-free medium the growth of primitive erythroid precursor cells is closely dependent on growth factor supply found in spleen conditioned medium. Using this medium allows us to clearly distinguish between the respective effects of erythropoietin and of BPA on erythroid progenitor cells.
D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D005260:Female; D006133:Growth Substances / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006418:Heme / Q000031:analogs & derivatives*; D006427:Hemin / Q000494:pharmacology* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C
D003470:Culture Media; D006133:Growth Substances; D006418:Heme; D006427:Hemin
Développement des progéniteurs érythroïdes précoces (BFUe) de la moelle osseuse de Souris dans un milieu de culture dépourvu de sérum. Effet de l'hémine.