title: Pristinamycin accumulation by Staphylococcus aureus.
abstract: Pristinamycins IA and IIA (PIA and PIIA) accumulation by Staphylococcus aureus has been studied with two hydrogenated analogs, (H2)PIA and (H2)PIIA. Rapid accumulation of both antibiotics at 37 degrees C is observed and internal concentrations can reach up to 58-fold the external concentration; this accumulation cannot be reduced by either metabolic inhibitors or tetracycline. The synergistic activity of pristinamycins IA and IIA is not observed at the bacterial accumulation level. We propose that pristinamycins enter into bacteria by a passive diffusion process and that the internal concentration is maintained by binding of the antibiotic to the bacterial ribosomes.
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis; D005947:Glucose / Q000494:pharmacology; D007700:Kinetics; D010455:Peptides / Q000378:metabolism; D013211:Staphylococcus aureus / Q000378:metabolism*; D013696:Temperature; D014316:Tritium; D014475:Uncoupling Agents / Q000494:pharmacology; D014769:Virginiamycin
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D010455:Peptides; D014475:Uncoupling Agents; D014316:Tritium; D014769:Virginiamycin; D005947:Glucose