Random locomotion in dark-field microscopy of single granulocytes from venous blood, tissue and exudate. Methodological considerations and clinical applications.
The random locomotion of granulocytes was studied in dark-field microscopy, and a standardized procedure for the classification of granulocytes into rapid-moving, (RM), slow-moving (SM) and non-moving (NM) cells was developed. When granulocytes from blood donors were tested, 54% were RM, 21% SM and 25% NM with variation between repeated tests, acceptable for a semiquantitative method. When the test was applied in 124 patients with infectious and inflammatory diseases and hematological malignancies, a significantly higher number of NM blood cells was found in patients with septicemia and pneumonia. The method was found especially suitable for samples containing few granulocytes, such as samples from soft-tissue infections, pulmonary infiltrates and neutropenic blood.
D000017:ABO Blood-Group System; D000293:Adolescent; D000328:Adult; D001065:Appendix / Q000166:cytology; D001773:Blood Cells / Q000145:classification; D002465:Cell Movement; D002470:Cell Survival; D005122:Exudates and Transudates / Q000166:cytology; D006098:Granulocytes / Q000145:classification*; D006801:Humans; D008168:Lung / Q000166:cytology; D008722:Methods; D008875:Middle Aged; D012204:Rh-Hr Blood-Group System; D013696:Temperature
D000017:ABO Blood-Group System; D012204:Rh-Hr Blood-Group System