title: Stimulation by BW755C and inhibition by cysteamine of duodenal epithelial alkaline secretion suggest a role of endogenous prostaglandin in mucosal protection.
abstract: Alkaline secretion by 12 mm segments of duodenum just distal to the Brunner's glands area was titrated in situ in anesthetized rats. Intravenous injection of the lipoxygenase inhibitor BW755C (10-20 mg/kg) increased the surface epithelial HCO3- secretion and pretreatment with indomethacin prevented this effect. This supports the view that endogenous production of prostaglandins is important in control of duodenal epithelial HCO3- secretion and ulceroprotection . Pretreatment with cysteamine (100 mg/kg) inhibited the ability of the duodenal surface epithelium to respond to luminal acid with a compensatory rise in alkaline secretion. Measurement of pH at the mucosal surface with microelectrodes revealed that acidification of this surface occurred simultaneously with the decline in alkaline secretion.
mesh_terms: D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001639:Bicarbonates / Q000378:metabolism*; D003543:Cysteamine / Q000494:pharmacology*; D004386:Duodenum / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007213:Indomethacin / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011453:Prostaglandins / Q000502:physiology*; D011720:Pyrazoles / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D000893:Anti-Inflammatory Agents; D001639:Bicarbonates; D011453:Prostaglandins; D011720:Pyrazoles; D003543:Cysteamine; D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D007213:Indomethacin