Effects of butylated hydroxyanisole and butylated hydroxytoluene on 7,12-dimethylbenz[a]anthracene-DNA adduct formation in mouse embryo cell cultures.
Neither butylated hydroxyanisole (BHA) nor butylated hydroxytoluene (BHT) significantly reduced overall 7,12-dimethylbenz[a]anthracene (DMBA)--DNA adduct formation in mouse embryo cell cultures. However, analysis of DMBA--DNA adducts by Servacel DHB chromatography and high-pressure liquid chromatography showed that treatment of cells with BHA, but not with BHT, resulted in a decreased contribution from the syn bay region dihydrodiol epoxide to overall binding.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000378:metabolism*; D000818:Animals; D000840:Anisoles / Q000494:pharmacology*; D001551:Benz(a)Anthracenes / Q000378:metabolism*; D002083:Butylated Hydroxyanisole / Q000494:pharmacology*; D002084:Butylated Hydroxytoluene / Q000494:pharmacology*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D004622:Embryo, Mammalian; D051379:Mice
D000840:Anisoles; D001551:Benz(a)Anthracenes; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole; D015127:9,10-Dimethyl-1,2-benzanthracene; D004247:DNA