Ultrastructural changes in the embryonic cells of the frog Microhyla ornata after cytochalasin H treatment.
Biological effects of cytochalasin H (CH), a newly isolated mould metabolite, have been found to bring about disaggregation of embryonic cells and to inhibit cytokinesis. Disaggregation is known to be a phenomenon related to the cell surface. (The cells are held together by a mucopolysaccharide glycoprotein complex.) In the present work the fact that the mucopolysaccharide glycoprotein surface coat gets affected by CH treatment is confirmed by electron microscopy with the help of Lanthanum, a specific marker, which gets selectively absorbed to the cell surface material and renders it electron dense. The ultrastructural observations indicated the reduction of the cell surface material in treated embryos as compared to the controls. The reappearance of lanthanum-bound cell surface material in the recovered embryos was also observed. However, the exact mechanism of the action of CH on the cell surface remains to be clarified.
D000818:Animals; D001001:Anura / Q000196:embryology*; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D003572:Cytochalasins / Q000494:pharmacology*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000187:drug effects*; D005110:Extracellular Space / Q000187:drug effects; D006003:Glycogen / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D007811:Lanthanum / Q000378:metabolism
D003572:Cytochalasins; D006025:Glycosaminoglycans; D007811:Lanthanum; D006003:Glycogen