Hemolysis in gel test for detection of bovine antibodies to Brucella abortus: comparison of antigens and test modifications.
Killed Brucella abortus strain 1119-3 cells were treated with hot phenol/water or dimethyl sulfoxide to extract soluble crude lipopolysaccharide antigen. Antigen preparations were characterized with respect to protein and lipopolysaccharide content and were compared for efficacy in the hemolysis-in-gel test (HIGT) for detecting anti-Brucella antibodies. All antigens were equally effective in sensitizing bovine RBC in the HIGT and in detecting the presence of anti-Brucella antibodies. A slide modification of the HIGT was developed and compared with the plate HIGT. Seemingly, both tests were comparable.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000032:analysis; D002003:Brucella abortus / Q000276:immunology; D002007:Brucellosis, Bovine / Q000276:immunology*; D002417:Cattle; D006462:Hemolytic Plaque Technique; D008070:Lipopolysaccharides / Q000032:analysis
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008070:Lipopolysaccharides