Identification of a yellow gene-specific protein in Drosophila melanogaster by two-dimensional gel electrophoresis.
Analysis of temperature-sensitive mutants suggests that the yellow (y) gene in Drosophila melanogaster is expressed at a different time in each cell type that gives rise to the various structures of the adult cuticle. An important step in analyzing the regulation of this gene requires identification of the y structural protein. A polypeptide has been identified which correlates with the presence or absence of a functional y gene. Furthermore, this protein has the tissue distribution profile expected of the y structural gene product. The ability to locate this gene was facilitated by the use of coisogenic stocks, two-dimensional electrophoretic protein separation, and an ultrasensitive silver protein stain.
D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D009154:Mutation; D010858:Pigmentation; D011506:Proteins / Q000235:genetics* / Q000302:isolation & purification; D013696:Temperature
D011506:Proteins