Effects of sulphydryl compounds on the radiation damage in biologically active DNA.
The effect of sulphydryl compounds on the induction of alkali-labile sites and on the contribution of such sites to the inactivation of single-stranded phi X174 DNA was studied. Cysteamine is capable of reacting with DNA radicals, thereby modifying the radiation damage in such a way that the induction of immediate and latent breaks is reduced. This depends on the pH of the solution. With cysteine only, a pH dependent protection, against lethal alkali-labile potential breaks could be observed. The damage other than breaks is not influenced by the presence of sulphydryl compounds.
D000468:Alkalies / Q000494:pharmacology; D010584:Bacteriophage phi X 174 / Q000187:drug effects / Q000528:radiation effects*; D003543:Cysteamine / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000528:radiation effects; D004279:DNA, Viral / Q000528:radiation effects*; D006863:Hydrogen-Ion Concentration; D011837:Radiation-Protective Agents; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D013696:Temperature
D000468:Alkalies; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D011837:Radiation-Protective Agents; D013438:Sulfhydryl Compounds; D003543:Cysteamine; D003545:Cysteine