Effect of particle size of dried mycobacteria on adjuvant induced arthritis in the rat.
Freund's complete adjuvant prepared by grinding dried, heat killed Mycobacterium tuberculosis with liquid paraffin was investigated for particle size-weight distribution. Variations in the grinding times and preparative procedure had a marked effect on the arthritogenic action of the adjuvant in the rat. A satisfactory response in the injected foot was found only when the particle size was less than 19 microns and a secondary response in the non-injected foot required particles less than 10 microns in diameter.
D000818:Animals; D001168:Arthritis / Q000276:immunology*; D001169:Arthritis, Experimental / Q000276:immunology*; D005620:Freund's Adjuvant; D008297:Male; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010316:Particle Size; D051381:Rats; D011919:Rats, Inbred Strains
D005620:Freund's Adjuvant