[Radioautographic demonstration of the methods of incorporation of tritiated leucine into germ cells of the testis incubated in a modified Rose chamber].
The influence of the blood-testis barrier on the synthetic capabilities of male germ cells in the mouse as investigated by quantitative radioautography in aggregates of seminiferous tubules incubated in a modified Rose chamber, in the presence of tritiated leucine. In the seminiferous tubule, a distribution gradient of radioactivity could be seen : radioactivity decreased from the periphery towards the lumen. Labelling intensity was approximately equivalent in type B spermatogonia and pachytene primary spermatocytes but was only one third as heavy in spermatids (steps 4-5). These results which confirm prior observations carried out in both animal and germ cells isolated from the seminiferous epithelium by differential sedimentation velocity indicate that the blood-testis barrier has no significant effect on the capacity for synthesis peculiar to each germ-cell population.
D000818:Animals; D001345:Autoradiography; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism*; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D013094:Spermatozoa / Q000378:metabolism* / Q000648:ultrastructure; D013737:Testis / Q000378:metabolism*; D014316:Tritium
D014316:Tritium; D007930:Leucine
Mise en évidence radioautographique des modalités d'incorporation de la leucine tritiée dans les cellules germinales du testicule incubé en chambre de Rose modifiée.