title: Region-specific recombination in phage T4. II. Structure of the recombinants.
abstract: In this paper, we present results of crosses designed to elucidate the structure of recombinants in the tail-fiber region of bacteriophage T4, in which a glucosylation-dependent recombinations mechanism is operative, and the cause of the "special" recombination in glycosylated crosses is discussed. We present evidence that, when phage are nonglycosylated, recombination in the tail-fiber region proceeds via long heteroduplex overlaps. Mismatched bases within such regions (in nonglycosylated phage) are repaired efficiently (as contrasted to those of glucosylated phage), but asymmetrically; that is, there may be an equal probability of resolving the mismatch to mutant or wild type.
mesh_terms: D002213:Capsid / Q000235:genetics; D002874:Chromosome Mapping; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D005964:Glucosyltransferases / Q000378:metabolism*; D006358:Hot Temperature; D008957:Models, Genetic; D009154:Mutation; D011995:Recombination, Genetic; D013604:T-Phages / Q000235:genetics*
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral; D005964:Glucosyltransferases