Separation of dimethylaminoazobenzenethiohydantoin amino acids by high-performance liquid chromatography at low picomole concentrations.
The separation of all common dimethylaminoazobenzenethiohydantoin (DABTH) amino acids derived from modified Edman sequencing can be achieved by using high-performance liquid chromatography. All derivatives, including DABTH-Ile and DABTH-Leu, can be readily separated in a solvent mixture of sodium acetate buffer and 1% ethylene dichloride in acetonitrile. The high absorbance of the DABTH amino acids at 436 nm makes possible the quantitative determination of these derivatives at picomole concentrations in a relatively short time (30-40 min).
D000097:Acetonitriles; D000596:Amino Acids / Q000302:isolation & purification*; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005025:Ethylene Dichlorides; D006827:Hydantoins / Q000302:isolation & purification*; D008832:Microchemistry; D012997:Solvents; D013053:Spectrophotometry; D013867:Thiohydantoins / Q000302:isolation & purification*; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives* / Q000302:isolation & purification
D000097:Acetonitriles; D000596:Amino Acids; D002021:Buffers; D005025:Ethylene Dichlorides; D006827:Hydantoins; D012997:Solvents; D013867:Thiohydantoins; C024565:ethylene dichloride; C022927:DABTH; D004124:p-Dimethylaminoazobenzene; C032159:acetonitrile