Polypeptide microsequence analysis with the commercially available gas-phase sequencer.
An evaluation of the commercially available gas-phase sequencer with regard to its sensitivity, ease of operation, and reliability is presented. Techniques for the preparation of ultra-pure reagents and solvents for this new technology are reviewed, as is a highly sensitive reverse-phase liquid chromatography program for the identification and quantitation of phenylthiohydantoin amino acid residues from the new sequenator.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods; D013007:Growth Hormone-Releasing Hormone / Q000032:analysis; D006801:Humans; D008832:Microchemistry; D010179:Pancreas / Q000032:analysis; D010455:Peptides
D000596:Amino Acids; D010455:Peptides; D013007:Growth Hormone-Releasing Hormone