Are different proteins synthesized in distinct domains in a neuron?
Are different messenger RNA species translated in distinct, limited domains within a cell? For the particular case of the giant R2 neuron from Aplysia californica an answer to this question is possible for the more abundantly synthesized proteins. After brief labeling with 35S-methionine, the R2 neuron soma was frozen and divided into two or three parts. The newly synthesized proteins were analyzed following two-dimensional polyacrylamide gel electrophoresis. No evidence for limited domains of synthesis for 26 abundantly synthesized polypeptides in the R2 neuron was found.
D000818:Animals; D001048:Aplysia; D002451:Cell Compartmentation; D004591:Electrophoresis, Polyacrylamide Gel; D007381:Intermediate Filament Proteins / Q000096:biosynthesis; D008715:Methionine / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D016900:Neurofilament Proteins; D009474:Neurons / Q000378:metabolism*; D010452:Peptide Biosynthesis; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis*
D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; D012333:RNA, Messenger; D008715:Methionine