Interleukin 3 augments the murine primary cytolytic T lymphocyte response to allogeneic tumor cells.
The primary anti-H-2k allospecific cytolytic T lymphocyte (CTL) response by BALB/c (H-2d) spleen cells in vitro to x-irradiated RDM4 (H-2k) tumor cells is weak. This response has been shown to be augmented by CTL helper factor (CHF), a factor present in supernatants of spleen cells cultured with Sendai virus (SC-CM). Conditioned medium from WEHI-3 cells (WEHI-CM) also contains activity that augments the BALB/c anti- RDM4 CTL response. Attempts to separate the CHF activity from interleukin 3 (IL 3), also present in WEHI-CM, were unsuccessful. Purified IL 3 was then tested, and was found to increase the BALB/c anti- RDM4 CTL response by five- to 10-fold. IL 3 is apparently the only material in WEHI-CM that is active in this assay. The response is apparently a classical CTL response because: 1) the effector cells are sensitive to monoclonal anti-Thy-1.2 antibody plus C; 2) the response is dependent on antigen stimulation, and it peaks on day 5 or 6 of culture; and 3) the effector cells are specific for H-2k targets. IL 3 must be added very early during the in vitro culture period for maximal augmentation of the response, consistent with possible action of IL 3 as a differentiation factor.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000276:immunology; D003470:Culture Media; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006358:Hot Temperature; D007376:Interleukin-2 / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology; D007377:Interleukin-3; D007519:Isoantigens / Q000276:immunology; D007700:Kinetics; D008222:Lymphokines / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014357:Trypsin / Q000494:pharmacology
D003470:Culture Media; D007376:Interleukin-2; D007377:Interleukin-3; D007519:Isoantigens; D008222:Lymphokines; D014357:Trypsin