Affinity chromatographic studies on antigen-antibody dissociation.
An analytical affinity chromatography assay has been developed for the investigation of the dissociation of antigen-antibody complexes. Albumin-coupled Sepharose 4B and anti-albumin has been used as a model system. At extremely low or high pH, in the presence of highly concentrated chaotropic ions at pH 7 or by elution with 100% ethylene glycol after pretreating with high pH buffer, most of the bondings could be ruptured. The latter two-step desorption procedure provides recovery of intact antibody with high yield. The technique was also utilized for the preparation of antibody against human growth hormone.
D000936:Antigen-Antibody Complex / Q000032:analysis*; D002846:Chromatography, Affinity / Q000379:methods; D013006:Growth Hormone / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D012709:Serum Albumin / Q000032:analysis / Q000276:immunology
D000936:Antigen-Antibody Complex; D012709:Serum Albumin; D013006:Growth Hormone