[Characterization of smooth muscle organs by determination of cellular myosin content].
The mean relative content of myosin of cells from smooth muscular organs was estimated using immunocytochemical methods after enzymatical tissue disintegration. The cells were classified according to myosin content. Aortas and urinary bladders of newborn rats and chicken amnions were used as models. Cells of a permanent cell strain, being free of smooth muscular myosin served as negative controls. It could be shown, that the content of myosin, estimated by immunocytochemical methods correlates with the state of development of smooth muscular organs.
D000650:Amnion / Q000032:analysis* / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D001011:Aorta / Q000032:analysis* / Q000254:growth & development; D002642:Chick Embryo; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D024510:Muscle Development; D009130:Muscle, Smooth / Q000032:analysis* / Q000166:cytology / Q000254:growth & development; D009218:Myosins / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D001743:Urinary Bladder / Q000032:analysis* / Q000254:growth & development
D009218:Myosins
Charakterisierung glattmuskulärer Organe mit Hilfe der Bestimmung des Myosingehaltes der Zellen.