Immunoassays for serum C-reactive protein employing fluorophore-labelled reactants.
Two simple rapid and precise fluorescence assays for determining serum levels of C-reactive protein (CRP) are described which employ sheep antibodies to CRP covalently linked to magnetisable cellulose/iron oxide particles. The first (a fluoroimmunoassay) is based on competitive binding of CRP in the sample or standard with fluorescein-labelled CRP, a 30 min incubation time, simple separation with a magnet followed by elution of the bound fraction into alkaline methanol and fluorescence quantitation. In the second (a 'sandwich' immunofluorometric assay) an excess of solid-phase linked antiserum is incubated with sample and fluorescein-labelled purified sheep anti-CRP immunoglobulin followed by separation, elution and quantitation of the bound fraction. The assays cover the ranges 3-400 mg/l and 3-70 mg/l respectively and the results correlate well with those obtained by radial immunodiffusion and radioimmunoassay.
D002097:C-Reactive Protein / Q000032:analysis* / Q000276:immunology / Q000592:standards; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D005779:Immunodiffusion; D011863:Radioimmunoassay; D013861:Thiocyanates
D005452:Fluoresceins; D007106:Immune Sera; D013861:Thiocyanates; D002097:C-Reactive Protein; D016650:Fluorescein-5-isothiocyanate