Blocking induction of human cytotoxic T lymphocytes with anti-Ia xenoantiserum.
A xenoantiserum to human Ia antigens has been described that is capable of blocking not only stimulation in the mixed lymphocyte reaction (MLR) but also the induction of cytotoxic T lymphocytes (CTLs). Data from immunofluorescence as well as complement-dependent cytolytic assays indicate that the anti-Ia xenoantiserum is directed against B cell surface antigens. Inhibition of complement-dependent cytolysis with column fractions of B cell antigens and autoradiography of immune precipitates electrophoresed on sodium dodecyl sulfate (SDS)-polyacrylamide gels have established that the antigen detected by the xenoantiserum has characteristics of the human two-polypeptide Ia molecular complex. Allogeneic stimulator cells pretreated with anti-Ia at very low doses were unable to stimulate in the MLR and failed to induce CTLs. Neither anti-beta 2-microglobulin nor a non-HLA-associated antilymphocyte serum in similar dose ranges inhibited MLR or cell-mediated lympholysis (CML) assays. Absorption of anti-Ia xenoantiserum with B lymphoblasts, but not T lymphoblasts, removed inhibitory activity for both MLR and CML. Untreated third-party stimulator cells cocultivated with anti-Ia-pretreated stimulator cells provided stimulation in the MLR that apparently allowed partial recovery of CML against targets from the same donor as the anti-Ia-treated stimulator cells. Elimination of the helper effect, normally provided by MLR stimulation, may be one mechanism by which anti-Ia xenoantiserum prevents induction of CTLs.
D000818:Animals; D001345:Autoradiography; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D003165:Complement System Proteins / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D007519:Isoantigens / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D011817:Rabbits / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D006680:HLA Antigens; D007106:Immune Sera; D007519:Isoantigens; D003165:Complement System Proteins