Effect of malignant transformation, retinoic acid, trifluoperazine, and N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W7) on the sensitivity of rodent cells to Pseudomonas toxin.
A number of mouse and rat cells and their virus-transformed counterparts were tested for sensitivity to Pseudomonas aeruginosa exotoxin A (PEA). In each case, the transformed cells were considerably less sensitive than were the nontransformed cells. In the presence of trifluoperazine, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide, or retinoic acid, the transformed cells became as sensitive as the nontransformed cells, whereas these drugs had little or no effect on the sensitivity to PEA of the nontransformed cells. Temperature-sensitive virus-transformed normal rabbit kidney cells were sensitized to PEA by N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide, when these cells were grown as the transformed phenotype, whereas the nontransformed phenotype could not be sensitized. The possibility is discussed that upon malignant transformation a process which is dependent upon calmodulin or protein kinase C strongly decreases the sensitivity of the cells to PEA.
D036002:ADP Ribose Transferases; D000818:Animals; D001427:Bacterial Toxins; D002147:Calmodulin / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D006224:Cricetinae; D005098:Exotoxins / Q000633:toxicity*; D007668:Kidney; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D014176:Protein Biosynthesis / Q000187:drug effects; D011550:Pseudomonas aeruginosa; D051381:Rats; D013449:Sulfonamides / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology*; D014268:Trifluoperazine / Q000494:pharmacology*; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D002147:Calmodulin; D005098:Exotoxins; D013449:Sulfonamides; D014212:Tretinoin; D014268:Trifluoperazine; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A; C017967:W 7