Forskolin and mouse oocyte maturation in vitro.
Oocytes isolated from mature follicles undergo spontaneous maturation when cultured in vitro. Forskolin, an adenylate cyclase stimulator, inhibited resumption of meiosis of cumulus-free mouse oocytes in vitro. Germinal vesicle breakdown (GVBD) was prevented in more than 85% of the oocytes treated by forskolin at concentrations of 20 micrograms/ml and higher. The inhibiting effect of forskolin was dose-dependent and reversible. FSH, LH, FSH plus LH, estrogen, progesterone, and estrogen plus progesterone did not reverse the block induced by forskolin in cumulus-free and cumulus-enclosed oocytes. The present results suggest that intracellular cAMP may play a role in the regulation of oocyte maturation.
D000818:Animals; D005576:Colforsin; D003470:Culture Media; D004054:Diethylstilbestrol / Q000494:pharmacology; D004224:Diterpenes / Q000494:pharmacology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000494:pharmacology; D051379:Mice; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development*; D011374:Progesterone / Q000494:pharmacology
D003470:Culture Media; D004224:Diterpenes; D005576:Colforsin; D011374:Progesterone; D004054:Diethylstilbestrol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone