Synthesis of glycosaminoglycans by chick retinal pigment epithelium in vitro.
Cultured chick embryonal retinal pigment epithelial (RPE) cells synthesized two main glycosaminoglycans (GAGs), chondroitin 4-sulfate, and heparan sulfate. Chondroitin 4-sulfate was liberated mainly in the medium (apical side) and heparan sulfate was incorporated into the cell and/or basement membrane. Glycosaminoglycans liberated into the medium were composed of chondroitin 4-sulfate (44%), keratan sulfate (22%), heparan sulfate (18%) and hyaluronate (12%). On the other hand, the GAGs in the cell and/or basement membrane were composed of heparan sulfate (52%), hyaluronate (19%), and chondroitin 4-sulfate (13%).
D000818:Animals; D002642:Chick Embryo; D002809:Chondroitin Sulfates / Q000096:biosynthesis / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006493:Heparin / Q000378:metabolism; D006497:Heparan Sulfate / Q000096:biosynthesis / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism; D007632:Keratan Sulfate / Q000378:metabolism; D010857:Pigment Epithelium of Eye / Q000378:metabolism*
D002809:Chondroitin Sulfates; D006025:Glycosaminoglycans; D006493:Heparin; D006497:Heparan Sulfate; D006820:Hyaluronic Acid; D007632:Keratan Sulfate