Flow cytometric analysis of immunogold cell surface label.
Goat antimouse immunoglobulin antibodies conjugated to colloidal 40-nm gold particles were used to label mouse spleen lymphocytes. The labeled cells were analysed with a flow cytometer, equipped with an argon-ion laser and a (0.5 mW) helium-neon laser. The right-angle (90 degrees) light scatter signal of the red (632.8 nm) helium-neon light was enhanced more than tenfold by the gold label. Dual labeling with gold and fluorescein isothiocyanate (FITC) showed no interference between the two labels. Thus immunogold provides a nonfluorescent cell surface label that can be combined with other cell labels for multiparametrical cell analysis.
D000818:Animals; D000906:Antibodies; D000954:Antigens, Surface; D002462:Cell Membrane / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006046:Gold; D007834:Lasers; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012542:Scattering, Radiation; D013154:Spleen / Q000276:immunology; D013861:Thiocyanates
D000906:Antibodies; D000954:Antigens, Surface; D005452:Fluoresceins; D013861:Thiocyanates; D006046:Gold; D016650:Fluorescein-5-isothiocyanate