Determination of immunoreactive melatonin in the colon of the rat by immunocytochemistry.
Melatonin immunofluorescence in the rat colon was measured with a cadmium sulfide photometer in conjunction with an immunohistological double antibody procedure. The method proved to be very reliable with 5-10% intra- and interassay reliabilities. In addition, manipulation of the primary antibody concentration, the incubation time, and the nature of the first antibody produced predicted changes in fluorescence intensity. Treatment with p-chlorophenylalanine led to a marked reduction of melatonin-specific fluorescence.
D000818:Animals; D003106:Colon / Q000032:analysis*; D004848:Epithelium / Q000032:analysis; D005260:Female; D010134:Fenclonine / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D008297:Male; D008550:Melatonin / Q000032:analysis* / Q000276:immunology; D051381:Rats
D007106:Immune Sera; D008550:Melatonin; D010134:Fenclonine