[Pertussis toxin stimulates brain adenyl cyclase and induces ADP ribosylation of a 40,000 dalton membrane protein].
The adenylate cyclase activity of Rat brain synaptosomal membranes is strongly activated by forskolin. This study demonstrates: 1. That GTP or its analogs, when added to forskolin, decrease the enzyme stimulation by a factor of 40 to 60%; 2. That Pertussis toxin (TP), in the presence of NAD, ADP-ribosylates a single membrane protein of 40,000 dalton apparent molecular weight in a strictly concomitant manner. This Lubrol-soluble protein has a sedimentation coefficient of 3.6 and a Stokes radius of 6 nm. An immune serum against TP blocks the NAD-glycohydrolase activity of the toxin.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D001921:Brain / Q000201:enzymology*; D005576:Colforsin; D004224:Diterpenes / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D037342:Pertussis Toxin; D051381:Rats; D013570:Synaptic Membranes / Q000201:enzymology; D010566:Virulence Factors, Bordetella
D037361:Adenylate Cyclase Toxin; D001427:Bacterial Toxins; D004224:Diterpenes; D008565:Membrane Proteins; D009702:Nucleoside Diphosphate Sugars; D010566:Virulence Factors, Bordetella; D009243:NAD; D005576:Colforsin; D000246:Adenosine Diphosphate Ribose; D006160:Guanosine Triphosphate; D037342:Pertussis Toxin; D000262:Adenylyl Cyclases
La toxine pertussis stimule l'adényl-cyclase cérébrale et provoque l'ADP-ribosylation d'une protéine membranaire de 40 000 dalton.