Effect of 3-aminobenzamide on the induction of toxicity and transformation by ethyl methanesulfonate and methylcholanthrene in BALB/3T3 cells.
3-Aminobenzamide, a potent inhibitor of nuclear poly ADP-ribosyl synthetase, was tested for its ability to alter the toxic and/or transforming effects of ethyl methanesulfonate, methyl methanesulfonate and 3-methylcholanthrene in BALB/3T3 clone A31-1 cells. 3-Aminobenzamide enhanced the toxic effects of both ethyl methanesulfonate and methyl methanesulfonate in a dose dependent manner, but had minimal effects on 3-methylcholanthrene induced toxicity. Similarly, 3-aminobenzamide greatly enhanced ethyl methanesulfonate induced transformation while failing to enhance the transformation of BALB/3T3 clone A31-1 cells by 3-methylcholanthrene. These results stress the importance of poly ADP-ribosyl synthetase in repair of DNA damage and the chemical induction of transformation in vitro.
D000818:Animals; D001549:Benzamides / Q000633:toxicity*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004260:DNA Repair; D004347:Drug Interactions; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D008748:Methylcholanthrene / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism
D001549:Benzamides; D008748:Methylcholanthrene; C025160:3-aminobenzamide; D005020:Ethyl Methanesulfonate; D011065:Poly(ADP-ribose) Polymerases