Human bone marrow fibroblast proliferation and GM-CFC colony formation in patients with acute leukemia.
The proliferation of bone marrow (BM)-derived fibroblasts and granulocyte-macrophage progenitors (GM-CFC) was studied in culture in 23 patients with acute leukemia (AL). The number of GM-CFC colonies was significantly lower in patients with infiltrated BM when compared to those in remission: 17 +/- 12 vs. 125 +/- 35 colonies per 2 X 10(5) cells (p less than 0.005). The proliferation of fibroblasts derived from leukemic BM was significantly more active in acute nonlymphocytic (ANLL) than in acute lymphoblastic leukemia (ALL): 6.8 +/- 3.2 X 10(5) vs. 2.8 +/- 1.8 X 10(5) fibroblasts per flask (p less than 0.05). The reduced GM-CFC colony formation correlated significantly (r = 0.84; p less than 0.02) with the reduced fibroblast proliferation in patients with ALL but not in those with ANLL. Supernatants from fibroblast monolayers stimulated additionally normal GM-CFC cultured in the presence of a potent conditioned medium as the source of colony stimulating activity. Fibroblast supernatants derived from BM with leukemic infiltrates, stimulated the incorporation of 3H-thymidine by monolayers of normal bone marrow fibroblasts but had no effect on ANLL fibroblasts.
D000208:Acute Disease; D001854:Bone Marrow Cells; D002455:Cell Division; D005347:Fibroblasts / Q000166:cytology*; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007938:Leukemia / Q000097:blood*; D007945:Leukemia, Lymphoid / Q000097:blood; D008264:Macrophages / Q000166:cytology