Inhibition of mitochondrial oxidative phosphorylation by 2-methyl-4-dimethylaminoazobenzene.
The azodye 2-methyl-4-dimethylaminoazobenzene inhibited oxidation and phosphorylation in tightly coupled rat liver mitochondria. Phosphorylation was more sensitive to the inhibitory action of the azodye than was the oxidation of succinate or ascorbate. The oxidation of NAD+-linked substrate was severely inhibited by the compound. In submitochondrial particles, only NADH oxidation was sensitive. The site of inhibition has been identified to lie between the dehydrogenase flavoprotein and ubiquinone.
D000818:Animals; D007700:Kinetics; D008749:Methyldimethylaminoazobenzene / Q000494:pharmacology*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D051381:Rats; D012402:Rotenone / Q000494:pharmacology; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid; D014451:Ubiquinone / Q000494:pharmacology; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives*
D013386:Succinates; D012402:Rotenone; D009243:NAD; D014451:Ubiquinone; D008749:Methyldimethylaminoazobenzene; D004124:p-Dimethylaminoazobenzene; D019802:Succinic Acid