title: [15N-labeled lysine in colostomized laying hens. 5. 15N incorporation into blood fractions and the gastrointestinal tract].
abstract: In the metabolism experiment three colostomized laying hens received, together with a commercial ration of 120 g, 0,2% 15N-labelled L-lysine with an atom-% 15N-excess (15N') of 48%; subsequently the same ration was fed over a period of 4 days with 0.2% unlabelled L-lysine. After the end of the experiment the hens were slaughtered. The atom-% 15N' was determined in total, in the lysine, histidine and arginine N in the corpuscles, the plasma, the NPN-fraction of the blood in the stomachs, the small intestine, the caecum and the rectum. 15N' in the corpuscles was 0.11 atom-%, in the blood plasma 0.17 atom-%, in the NPN-fraction of the blood 0.09 atom-%, in the tissue of the gastro-intestinal tract 0.11 atom-% and in its contents 0,12 atom-%. On average the blood contained per hen 77.9% lysine-15N', 16.4% arginine-15N' and 5.7% histidine-15N' of the basic amino acid-15N'. For the gastro-intestinal tract 78.7% lysine-15N', 19.0% arginine-15N' and 2.3% histidine-15N' of the 15N' of the basic amino acids were ascertained. In comparison to histidine the alpha-amino-N of lysine is incorporated to a considerably higher degree into arginine. For lysine and arginine the atom-% 15N' in the contents of the gastro-intestinal tract for days after the end of the supplementation of labelled lysine is between 8 and 10 times higher than in the feces of the last day of the experiment. This indicates a considerable secretion of the two amino acids in the gastro-intestinal tract and their re-absorption to a large extent.
mesh_terms: D000596:Amino Acids / Q000097:blood / Q000378:metabolism; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D003125:Colostomy / Q000662:veterinary; D004064:Digestive System / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D008239:Lysine / Q000378:metabolism*; D009584:Nitrogen / Q000097:blood / Q000378:metabolism; D009587:Nitrogen Isotopes; D010949:Plasma / Q000378:metabolism
chemical_list: D000596:Amino Acids; D009587:Nitrogen Isotopes; D008239:Lysine; D009584:Nitrogen
vernacular_title: Untersuchungen mit 15N-markiertem Lysin bei kolostomierten Legehennen. 5. Mitteilung. 15N-Inkorporation in die Blutfraktionen und den Gastrointestinaltrakt.