title: Simple method for preparation of cryoprecipitate (CP) and cryodepleted plasma (CDP).
abstract: Cryoprecipitates (CP) and cryodepleted plasma (CDP) were prepared from fresh frozen plasma (FFP). Plasma was easily and cleanly frozen at -50 degrees C using a methanol-bath Ultracryostat, which has been available commercially for the past few years. From a random sample (n = 6), factors VIII:C, IX:C, V:C, fibrinogen, antithrombin III and fibronectin were determined. Concerning the total contents and the in-vitro-recovery of factor VIII:C (x:104 IU/53.5%) and fibrinogen x:175 mg/36.9%), the preparation method was as efficient as other equally common methods. Apart from the well-known applications, CP may be used for the substitution of fibronectin (x:46.2 mg/73.0%). The supernatant plasma of cryoprecipitation (CDP) can be utilized for substitution of coagulation disorders especially deficiencies of the prothrombincomplex and antithrombin III (x:IX:C:183 IU/76.2%; V:C:140.5 U/73.8%; AT III:162 U/80.2%).
mesh_terms: D000990:Antithrombin III / Q000032:analysis; D011232:Chemical Precipitation; D005164:Factor IX / Q000032:analysis; D005165:Factor V / Q000032:analysis / Q000276:immunology; D005169:Factor VIII / Q000032:analysis; D005340:Fibrinogen / Q000032:analysis; D005353:Fibronectins / Q000032:analysis; D005615:Freezing; D006467:Hemophilia A / Q000628:therapy*; D006801:Humans; D010952:Plasma Substitutes / Q000138:chemical synthesis*; D018805:Sepsis / Q000628:therapy*; D014842:von Willebrand Diseases / Q000628:therapy*
chemical_list: D005353:Fibronectins; D010952:Plasma Substitutes; C035876:factor V clotting antigen; D000990:Antithrombin III; D005165:Factor V; D005169:Factor VIII; D005164:Factor IX; D005340:Fibrinogen