title: Effect of cortisol on periosteal and nonperiosteal collagen and DNA synthesis in cultured rat calvariae.
abstract: The effects of cortisol on bone formation are complex and may be modulated by the presence of periosteal cells or by factors released by the periosteal tissue. To test these possibilities, cortisol was examined for its effects on the incorporation of 3H-proline into collagenase-digestible protein (CDP) and noncollagen protein (NCP), on DNA synthesis and on alkaline phosphatase activity in intact and in the periosteum and nonperiosteal bone of dissected calvariae from 21-day-old fetal rats. After 24 h of treatment, cortisol increased the incorporation of 3H-proline into CDP in intact bones and in the nonperiosteal bone of calvariae dissected after the culture. Cortisol inhibited the incorporation of 3H-thymidine into calvarial DNA but it caused a small increase in nonperiosteal DNA content. Cortisol did not affect the incorporation of 3H-proline into CDP in calvariae dissected prior to the culture if the periosteum and nonperiosteal central bone were incubated separately; the stimulatory effect was observed only if the two tissues were cultured in the same vial and were in contact. In contrast, cortisol stimulated alkaline phosphatase activity in the central nonperiosteal bone of calvariae dissected before or after the culture. After 72-96 h of treatment, cortisol inhibited the labeling of CDP, NCP, and DNA and the DNA content in intact bones and in both periosteal and nonperiosteal central bone of calvariae dissected after the culture. In contrast, when the periosteum was removed before the incubation, these inhibitory effects were observed in the periosteum and not in the nonperiosteal bone.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism; D003094:Collagen / Q000096:biosynthesis*; D004247:DNA / Q000096:biosynthesis*; D006854:Hydrocortisone / Q000494:pharmacology*; D009924:Organ Culture Techniques; D010521:Periosteum / Q000187:drug effects* / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats; D012886:Skull / Q000196:embryology; D013997:Time Factors
chemical_list: D003094:Collagen; D004247:DNA; D000469:Alkaline Phosphatase; D006854:Hydrocortisone