Analysis of HLA-B27 antigen with monoclonal antibodies.
To explore the possibility that the HLA-B27 antigen may exist in more than one form, murine monoclonal antibodies were produced to B27 molecules. The first such antibody, anti-B27M1, reacts with 100% of B27 + cells and, therefore, does not distinguish B27 + healthy individuals from those with spondyloarthropathies. A second antibody, anti-B27M2, recognizes a variant of the B27 molecule that is present in most but not all Caucasian B27 + individuals. The frequency of B27M2(+) variants also appears to differ among different ethnic groups. Preliminary studies suggest that B27 + patients with ankylosing spondylitis are less likely than B27 + healthy individuals to express the B27M2 variant.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000920:Antibody-Dependent Cell Cytotoxicity; D004198:Disease Susceptibility; D005006:Ethnicity; D005802:Genes, MHC Class II; D006680:HLA Antigens / Q000276:immunology*; D015796:HLA-B27 Antigen; D006801:Humans; D066298:In Vitro Techniques; D007518:Isoantibodies / Q000096:biosynthesis*; D051379:Mice; D013167:Spondylitis, Ankylosing / Q000209:etiology* / Q000235:genetics / Q000276:immunology
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D015796:HLA-B27 Antigen; D007518:Isoantibodies