Binding of phencyclidine to rat brain membranes: technical aspect.
[3H]Phencyclidine has a tendency to adsorb to filters used in binding experiments. The spinal binding techniques that should be used to overcome this difficulty are described. The affinity of [3H]phencyclidine for its brain receptor was 4 times higher in a medium containing 5 mM salt than in a medium containing 50 mM salt.
D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D005374:Filtration / Q000379:methods; D066298:In Vitro Techniques; D009994:Osmolar Concentration; D010622:Phencyclidine / Q000378:metabolism*; D051381:Rats; D011955:Receptors, Drug / Q000378:metabolism; D017478:Receptors, Phencyclidine; D013570:Synaptic Membranes / Q000378:metabolism*
D011955:Receptors, Drug; D017478:Receptors, Phencyclidine; D010622:Phencyclidine