title: Human adult endothelial cell growth in culture.
abstract: The purpose of the present study was to culture human adult endothelial cells (HAECs) on a long-term basis in the laboratory. Previous inability to accomplish this has been the major impediment to the in vitro study of endothelialization of prosthetic grafts with human cells, a problem of significant clinical relevance. We have been successful in developing a technique that allows HAECs from human adult arteries, veins, and capillaries to proliferate vigorously in culture for up to 80 population doublings. HAECs are grown on a gelatin surface (medium 199 containing 20% fetal calf serum). Heparin and endothelial cell growth factor (ECGF) are required for optimal growth. With this technique, which will be described in detail, over 10(23) HAECs can be produced from each 1 cm2 of vascular tissue. This makes large numbers of HAECs available for high-density seeding on prosthetic grafts prior to implantation. It also permits for the first time with human cells the in vitro study of prosthetic grafts--HAEC interactions and the factors that enhance optimal growth and adherence to prosthetic materials. It is hoped that identification of the factors promoting graft endothelialization in combination with high-density seeding will lower graft thrombogenicity and therefore result in greater graft longevity than has been possible heretofore.
mesh_terms: D000328:Adult; D000941:Antigens / Q000032:analysis; D001807:Blood Vessel Prosthesis; D001808:Blood Vessels / Q000166:cytology; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D016228:Endothelial Growth Factors; D004727:Endothelium / Q000166:cytology*; D005169:Factor VIII / Q000032:analysis / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006133:Growth Substances / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D014841:von Willebrand Factor
chemical_list: D000941:Antigens; D003470:Culture Media; D016228:Endothelial Growth Factors; D006133:Growth Substances; D014841:von Willebrand Factor; D005169:Factor VIII; D006493:Heparin