Structural implications of primary sequences from a family of Balbiani ring-encoded proteins in Chironomus.
DNA sequencing has revealed an internal, tandemly repetitive structure in the family of giant polypeptides encoded by three types of Balbiani ring (BR) genes, in three different species of Chironomus. Each major BR repeat can be subdivided into two halves: a region consisting of short subrepeats and a more constant region that lacks obvious subrepeats. Comparative predictions of secondary structure indicate that an alpha-helical segment is consistently present in the amino-terminal half of the constant region in all known BR proteins. Comparative predictions, coupled with consideration of the known phosphorylation of serine and threonine residues in BR proteins, suggest that the alpha-helical structure may also extend into the carboxy-terminal half of the constant region, possibly interrupted by beta-turn(s). However, it is also possible that the structure is variable, and that a beta-strand is present in that half in some cases. All of the constant regions conserve one methionine and one phenylalanine residue, as well as all four cysteines; these residues presumably play roles in the packing or cross-linking of aligned constant regions. The structure of the subrepeat region is not clear, but the prevalence of a tripeptide pattern (basic-proline-acidic) suggests some type of structural regularity, possibly an extended helix. The possible significance of these conserved molecular features is discussed in the context of how they may serve the elasticity, insolubility, and hydrophilicity of the fibrils and threads formed by the BR polypeptides.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002683:Chironomidae / Q000235:genetics*; D004175:Diptera / Q000235:genetics*; D005796:Genes; D010802:Phylogeny; D011487:Protein Conformation; D012091:Repetitive Sequences, Nucleic Acid; D012471:Salivary Proteins and Peptides / Q000235:genetics*; D013045:Species Specificity
D012471:Salivary Proteins and Peptides