Radioimmunoassay for desmosine.
A radioimmunoassay was developed for the determination of desmosine. Desmosine conjugated to albumin was injected into rabbits which developed useful titers of serum antibodies after six months. A radioactive probe was prepared with desmosine using [125I]-Bolton-Hunter reagent. Bound desmosine was separated from free desmosine by cellulose acetate filter binding. The sensitivity of the assay is 1-50 picomoles of desmosine. The antibody is highly selective for desmosine, reacting less than 1% with other known crosslinks. Some prepurification may be necessary with complex samples which contain trace amounts of elastin peptides.
D000418:Albumins; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D006224:Cricetinae; D003895:Desmosine / Q000032:analysis* / Q000276:immunology; D004549:Elastin / Q000032:analysis; D006801:Humans; D008832:Microchemistry; D011817:Rabbits; D011863:Radioimmunoassay
D000418:Albumins; D000596:Amino Acids; D003895:Desmosine; D004549:Elastin