The effect of pretreatment of Escherichia coli CM891 with ethylenediaminetetraacetate on sensitivity to a variety of standard mutagens.
The effect of EDTA pretreatment on the sensitivity of E. coli CM891 to 10 standard mutagens was assayed for cytotoxicity, trp- reversion and mutation to A2Cr . There was no obvious correlation of effect with molecular weight but good correlation with water solubility. EDTA pretreatment did not improve the sensitivity of E. coli to agents which were very soluble in water viz. MMS, CP, ACR, DR and GM. However, with very water-insoluble agents, EDTA pretreatment usually gave a significant, 2-5-fold increase in mutation, viz. with 2NF , BP and CAP (trp locus) but not with 9AA . 2AP , which was moderately soluble in distilled water, only showed a small significant increase at the A2C locus after EDTA pretreatment. Increases in mutation were not always paralleled by increases in cytotoxicity.
D002470:Cell Survival / Q000187:drug effects; D004492:Edetic Acid / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects*; D008970:Molecular Weight; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000008:administration & dosage*; D012995:Solubility
D009153:Mutagens; D004492:Edetic Acid