Stimulation of the release of lysosomal and nonlysosomal granular enzymes from macrophages treated with monensin.
Mouse peritoneal macrophages that had been treated with a monovalent carboxylic ionophore, monensin, selectively secreted lysosomal and nonlysosomal granular enzymes into the medium. When macrophages were incubated with 1 to 10 microM monensin, the release of beta-glucuronidase, beta-hexosaminidase and beta-galactosidase was stimulated time and does dependently. Neither the beta-glucosidase nor acid phosphatase, enzymes bound to the lysosomal membranes, however, were released by monensin. Neutral alpha-glucosidase, shown recently to be localized in nonlysosomal granules of macrophages (15), was released by monensin at concentrations lower than those required for lysosomal enzyme release. Increased release of lysosomal enzymes also took place in a manner similar to that seen with monensin-treated macrophages after treatment of macrophages with weak bases, chloroquine and ammonium chloride. Neutral alpha-glucosidase, however, was not released when chloroquine was present in concentrations that stimulated the release of lysosomal enzymes. The UDP-galactosyltransferase activity of the Golgi apparatus in the macrophages markedly decreased after treatment with low concentration of monensin.
D000135:Acid Phosphatase / Q000378:metabolism; D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D002738:Chloroquine / Q000494:pharmacology; D005663:Furans / Q000494:pharmacology*; D005700:Galactosyltransferases / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D006056:Golgi Apparatus / Q000201:enzymology; D006596:Hexosaminidases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000201:enzymology*; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D008985:Monensin / Q000494:pharmacology*; D012995:Solubility; D000520:alpha-Glucosidases / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
D005663:Furans; D000643:Ammonium Chloride; D002738:Chloroquine; D008985:Monensin; D005700:Galactosyltransferases; D000135:Acid Phosphatase; D006596:Hexosaminidases; D000520:alpha-Glucosidases; D001617:beta-Glucosidase; D001616:beta-Galactosidase; D005966:Glucuronidase