Collection of mucosal secretion by synthetic discs for quantitation of secretory IgA and bacteria.
A practical method for collection and processing of mucosal secretion was developed to facilitate quantitation of its contents of secretory IgA (sIgA) and bacteria. Small discs were punched out from a commercially available synthetic electrophoresis wick. The discs absorbed a constant volume of saliva (52.4 +/- 3.9 microliters), and antibodies and encapsulated bacteria (Neisseria meningitidis) could subsequently be completely eluted. Serum IgA, IgM and IgG antibody activity was preserved both in discs stored dry for 3 days at 22 degrees C and in discs stored at -22 degrees C for 2 months. The secretory IgA concentrations of multiple samples of nasal secretion collected from 1 person were stable, demonstrating the reproducibility of the disc collecting method.
D000914:Antibodies, Viral / Q000032:analysis; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D009093:Mucus / Q000032:analysis*; D009297:Nasal Mucosa / Q000032:analysis*; D009345:Neisseria meningitidis / Q000302:isolation & purification*; D012409:Rubella / Q000276:immunology; D012463:Saliva / Q000276:immunology / Q000382:microbiology
D000914:Antibodies, Viral; D007071:Immunoglobulin A, Secretory