title: High-performance affinity chromatography: a rapid technique for the isolation and quantitation of IgG from cerebral spinal fluid.
abstract: Standard techniques for the quantitative measurement of IgG in cerebral spinal fluid take up to 24 hs. This often delays diagnosis and treatment, critical in newborn infants. A high-performance affinity chromatography (HPAC) column, containing immobilized anti-IgG antibody, produced the same or better results in 1 h. The HPAC system gave a 98% correlation with the standard techniques at the normal-abnormal IgG level, but was more accurate at the extremely low IgG level.
mesh_terms: D002508:Cephalometry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid* / Q000302:isolation & purification; D013205:Staphylococcal Protein A
chemical_list: D007074:Immunoglobulin G; D013205:Staphylococcal Protein A