Corn oil and its minor constituents as inhibitors of DMBA-induced chromosomal breaks in vivo.
Inhibitory effects of corn oil and its constituents have been studied against 7,12-dimethylbenz[a]anthracene (DMBA)-induced chromosomal breaks in B6C3F1 female mice using the in vivo bone-marrow micronucleus assay. We tested propyl gallate, alpha-tocopherol and beta-sitosterol as constituents of corn oil. In addition, sunflower oil was tested also to check whether corn oil differs from any other vegetable oil. Corn oil, propyl gallate, beta-sitosterol or sunflower oil were injected i.p. to mice for 2 days at 24-h intervals, prior to injecting DMBA i.p. alpha-Tocopherol was mixed in powdered food and the mice were fed on it for 4 days before receiving DMBA. Bone-marrow samples were collected at various 24-h intervals. About 50-70% reduction in number of micronucleated polychromatic erythrocytes (MNPCE)/500 PCEs were observed in all the treatments wherever corn oil was used. Significant inhibitory effects were noted in treatments with alpha-tocopherol and beta-sitosterol. Sunflower oil also showed an inhibitory effect, similar to that with corn oil.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity*; D000818:Animals; D001551:Benz(a)Anthracenes / Q000633:toxicity*; D001853:Bone Marrow / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects; D002869:Chromosome Aberrations; D003314:Corn Oil; D004041:Dietary Fats / Q000494:pharmacology*; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D009152:Mutagenicity Tests; D009821:Oils / Q000494:pharmacology*; D010938:Plant Oils; D011435:Propyl Gallate / Q000494:pharmacology; D012855:Sitosterols / Q000494:pharmacology; D000074242:Sunflower Oil; D014801:Vitamin A / Q000494:pharmacology
D001551:Benz(a)Anthracenes; D004041:Dietary Fats; D009821:Oils; D010938:Plant Oils; D012855:Sitosterols; D000074242:Sunflower Oil; D014801:Vitamin A; D015127:9,10-Dimethyl-1,2-benzanthracene; C025473:gamma-sitosterol; D003314:Corn Oil; D011435:Propyl Gallate