Increase in cytosolic Ca2+ concentration during t-butyl hydroperoxide metabolism by isolated hepatocytes involves NADPH oxidation and mobilization of intracellular Ca2+ stores.
Activation of phosphorylase a in hepatocytes incubated with t-butyl hydroperoxide indicates that hydroperoxide metabolism is associated with an increase in cytosolic free Ca2+ concentration which appears to be mediated by NADPH oxidation and to involve mobilization of intracellular Ca2+ stores.
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D004789:Enzyme Activation; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction; D010545:Peroxides / Q000378:metabolism*; D010762:Phosphorylase a / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D020122:tert-Butylhydroperoxide
D010545:Peroxides; D000001:Calcimycin; D009249:NADP; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D020122:tert-Butylhydroperoxide; D010762:Phosphorylase a; D002118:Calcium