Intestinal absorption of dinitrophenyl-lysine and effect of immunization with dinitrophenylated bovine serum albumin.
The intestinal absorption of dinitrophenyl-lysine (DNP-lys) was studied with a special interest on the role of the immune system in the absorption of small molecules which are recognized as nonself. [3H]-DNP-lys was rapidly absorbed by ligated intestinal loops in situ via a saturable and unique route. When [3H]-DNP-lys was preincubated with the immune serum obtained from rats immunized with dinitrophenylated bovine serum albumin (DNP-BSA), the [3H]-DNP-lys absorption was depressed. The absorption of [3H]-DNP-lys in DNP-BSA-immunized rats was depressed compared to the control. The results obtained suggest that the immune system play a role in avoiding the absorption of small molecules with antigenicity.
D000818:Animals; D004140:Dinitrophenols; D007114:Immunization; D007408:Intestinal Absorption; D007583:Jejunum / Q000378:metabolism; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012710:Serum Albumin, Bovine / Q000276:immunology*; D013997:Time Factors
D004140:Dinitrophenols; C034638:dinitrophenyl-bovine serum albumin; C007493:epsilon-dinitrophenyllysine; D012710:Serum Albumin, Bovine; D008239:Lysine