title: A collaborative study on the use of single radial immunodiffusion for the assay of rabies virus glycoprotein.
abstract: The single radial immunodiffusion (SRD) technique has been applied to the assay of the glycoprotein content of rabies vaccines produced in cell cultures. Fourteen laboratories in seven countries participated in a collaborative study to evaluate the reproducibility of the SRD technique; some laboratories also examined vaccines in the mouse protection (NIH) test and by enzyme immunoassay. Good agreement was found between potency estimates using the SRD technique: the geometric coefficients of variation for combined potency estimates of all laboratories were about 10%. SRD assays appear to have a role for the in vitro assay of antigen content of vaccine and could complement results obtained in in vivo assays which are subject to wide variability.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001681:Biological Assay; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000032:analysis*; D005779:Immunodiffusion; D051379:Mice; D011819:Rabies Vaccines / Q000276:immunology*; D013223:Statistics as Topic
chemical_list: D000956:Antigens, Viral; D006023:Glycoproteins; D011819:Rabies Vaccines