title: Fractionation of Aspergillus fumigatus antigens by hydrophobic interaction chromatography and gel filtration.
abstract: Aspergillus fumigatus somatic antigen prepared from young hyphae was fractionated by hydrophobic interaction chromatography followed by gel filtration. The antigenic activity of fractions was investigated by enzyme-linked immunosorbent assay, crossed immunoelectrophoresis, and immunodiffusion. By enzyme-linked immunosorbent assay, high levels of IgG antibodies were detected against an antigen fraction of approximate molecular weight 470,000 both in patients with aspergillosis (n = 3) and in healthy controls (n = 3). High levels of antibodies to four fractions of MW 250,000 160,000, 160,000-50,000, and 50,000-25,000 were found only in patients. The MW 250,000 fraction exhibited catalase activity, and the MW 50,000-25,000 fraction had neutral protease activity. These purified aspergillus antigens may be useful in serodiagnosis of aspergillosis.
mesh_terms: D000328:Adult; D000818:Animals; D000946:Antigens, Fungal / Q000032:analysis*; D001228:Aspergillosis / Q000276:immunology; D001232:Aspergillus fumigatus / Q000276:immunology*; D002374:Catalase / Q000378:metabolism; D005591:Chemical Fractionation; D002850:Chromatography, Gel / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D008297:Male; D008875:Middle Aged; D010447:Peptide Hydrolases / Q000378:metabolism; D011817:Rabbits
chemical_list: D000946:Antigens, Fungal; D006023:Glycoproteins; D002374:Catalase; D010447:Peptide Hydrolases