title: [Steady-state dependence of the methemoglobin reduction rate on its concentration in intact human erythrocytes].
abstract: The rate of methemoglobin reduction by the methemoglobin reductase system of intact human erythrocytes was measured as a rate of pyruvate formation in a quasi-steady state. Various methemoglobin concentrations (up to 100%) were generated by sodium nitrite additions. The steady state methemoglobin levels were maintained by infusion of a nitrite solution at a rate of 2.8 mmol/h/l cells. The rate of pyruvate formation was proportional to the steady state methemoglobin concentration in the range from the physiological value to 100%, the maximal value being as high as 500 mumol/h/l cells. It was found that the rate of CO2 output by the erythrocytes markedly increased in the presence of 8 mM sodium nitrite, reaching up to about 40% of the possible maximal value.
mesh_terms: D002245:Carbon Dioxide / Q000097:blood; D042966:Cytochrome-B(5) Reductase / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D008708:Methemoglobinemia / Q000097:blood*; D009247:NADH, NADPH Oxidoreductases / Q000097:blood*; D009566:Nitrates / Q000494:pharmacology; D010084:Oxidation-Reduction; D011773:Pyruvates / Q000097:blood
chemical_list: D009566:Nitrates; D011773:Pyruvates; D002245:Carbon Dioxide; C031618:sodium nitrate; D009247:NADH, NADPH Oxidoreductases; D042966:Cytochrome-B(5) Reductase
vernacular_title: Statsionarnaia zavisimost' skorosti vosstanovleniia metgemoglobina ot ego kontsentratsii v intaktnykh éritrotsitakh cheloveka.