Activation of phospholipase C and prostaglandin synthesis by [arginine]vasopressin in cultures.
[Arginine]vasopressin (AVP) stimulates maximal prostaglandin E2 production in cultured rat renal mesangial cells within 2 min. As early as 10s after addition of AVP (10(-6)M) a significant loss of radioactivity from phosphatidylinositol 4,5-bisphosphate but not from phosphatidylinositol 4-phosphate and phosphatidylinositol was observed in cells prelabelled with 32Pi. Cells labelled with [14C]arachidonic acid showed an increase of label in 1,2-diacylglycerol after 15 s and in phosphatidic acid after 30 s upon stimulation with AVP. Pretreatment of the cells with indomethacin (10(-5)M) did not abolish the effect of AVP on the increased labelling of phosphatidic acid.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001127:Arginine Vasopressin / Q000494:pharmacology*; D002478:Cells, Cultured; D015232:Dinoprostone; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007213:Indomethacin / Q000494:pharmacology; D010740:Phospholipases / Q000378:metabolism*; D011458:Prostaglandins E / Q000096:biosynthesis*; D051381:Rats; D013997:Time Factors; D010738:Type C Phospholipases / Q000378:metabolism*
D001095:Arachidonic Acids; D011458:Prostaglandins E; D001127:Arginine Vasopressin; D016718:Arachidonic Acid; D010740:Phospholipases; D010738:Type C Phospholipases; D015232:Dinoprostone; D007213:Indomethacin