title: Incorporation of fatty acids into phospholipids in L cells stimulated by antibody.
abstract: Binding antibodies to surface membranes stimulated incorporation of fatty acids (FA) into phospholipids of L cells. Antibodies stimulated at least a 3.4-fold greater incorporation of arachidonic acid into phosphatidylinositol than into any other class of phospholipid when compared on a molar basis (p less than 0.003). This enhanced incorporation was selective, depending on the character of the FA, because antibodies stimulated the incorporation of arachidonic acid at least 2.4-fold more than oleic acid, palmitic acid or stearic acid (p less than 0.001). Surprisingly, an antibody-stimulated incorporation of palmitic acid into sphingomyelin (SM) was at least 2.2-fold greater than that into any other class of phospholipid (p less than 0.001) and the antibody-stimulated incorporation of palmitic acid into SM was at least 60-fold greater than that of arachidonic acid, stearic or oleic acid (p less than 0.001). Nontoxic doses of ethylenediamine tetraacetic acid (EDTA), dexamethasone, 4-bromophenacylbromide and indomethacin inhibited the antibody-stimulated incorporation of arachidonic acid into cellular phospholipids, principally phosphatidylinositol (PI), and similarly inhibited the antibody stimulation of DNA synthesis. We conclude that when antibody binds to surface antigens on L cells, a rapid and selective incorporation of fatty acids into certain cellular phospholipids occurs, possibly mediated by calcium-dependent phospholipases. Degradation products of arachidonic acid, i.e., prostaglandins, may be important in these antibody stimulation events, as well. These early changes in phospholipid metabolism may serve as an important signal or mechanism for the subsequent stimulation of DNA synthesis in L cells.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001345:Autoradiography; D004247:DNA / Q000096:biosynthesis; D004492:Edetic Acid / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D007739:L Cells / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis*; D011817:Rabbits
chemical_list: D000906:Antibodies; D001095:Arachidonic Acids; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D010169:Palmitic Acids; D010743:Phospholipids; D016718:Arachidonic Acid; D019308:Palmitic Acid; D004247:DNA; D004492:Edetic Acid