Biosynthesis of plasma proteins in serum-free medium by primary monolayer culture of rat hepatocytes.
Morphologically intact rat hepatocytes separated by collagenase perfusion  were cultured in L-15/fetal calf serum medium to form a monolayer. Thereafter the hepatocytes were grown in serum-free L-15 medium in which they produced and continuously released plasma proteins. The secreted plasma proteins were collected, separated and characterized by crossed immunoelectrophoresis. Most of the newly biosynthesized plasma proteins secreted into the medium during incubation for thirty hours had the same electrophoretic mobility, antigenicity and staining characteristics as their counterparts in rat serum. The addition of tritium labelled amino acid mixture to the culture medium revealed that the release of radioactively labelled plasma proteins into the culture medium was essentially linear during the thirty hour incubation period. However, saturation of the intracellular pool took place after ca. ten hours of incubation. Addition of leukocytic endogenous mediator, LEM, to cultures of rat hepatocytes caused a profound increase in the relative concentration of acute-phase proteins secreted into the culture medium.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001798:Blood Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D002478:Cells, Cultured; D007123:Immunoelectrophoresis, Two-Dimensional; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D013047:Specific Pathogen-Free Organisms; D014316:Tritium
D000596:Amino Acids; D001798:Blood Proteins; D014316:Tritium