title: Guinea pig plasma kallikrein as a vascular permeability enhancement factor. Its dependence on kinin generation and regulation mechanisms in vivo.
abstract: Plasma kallikrein (mol wt 80,000) was purified from guinea pig plasma, and it caused vascular permeability enhancement when injected into guinea pig skin. The activity had a linear relationship to the logarithm of kallikrein concentrations from 5 X 10(-9) M to 5 X 10(-6) M and was blocked by immunopurified anti-prekallikrein F(ab')2 rabbit antibody and soybean trypsin inhibitor. Carboxypeptidase B(1.7 units), a kinin-destructive enzyme, decreased the permeability activity to 1/20, while SQ 20,881 (10(-6) M), an inhibitor to a kinin-destructive enzyme, augmented the activity 5.4-fold. These results suggested that the permeability activity of kallikrein was performed finally through kinin generation in the skin. The permeability activity was short-lasting, and was completely blocked by a kallikrein inhibitor purified from guinea pig plasma, suggesting the presence of a down-regulation system for the permeability activity in vivo. Prostaglandin E2 (25 ng), a hyperemia inducer in microcirculation, augmented the permeability activity 12-fold, suggesting the presence of an up-regulation system in vivo. Accordingly, it was assumed that kallikrein-kinin system might play a role as a vascular permeability enhancement system in guinea pig skin.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D002199:Capillary Permeability / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006168:Guinea Pigs; D005779:Immunodiffusion; D007610:Kallikreins / Q000302:isolation & purification / Q000378:metabolism*; D007705:Kinins / Q000378:metabolism*; D008297:Male; D011288:Prekallikrein / Q000276:immunology; D011458:Prostaglandins E / Q000494:pharmacology; D014361:Trypsin Inhibitors / Q000378:metabolism
chemical_list: D000906:Antibodies; D007705:Kinins; D011458:Prostaglandins E; D014361:Trypsin Inhibitors; D011288:Prekallikrein; D007610:Kallikreins