[Guerreiro Machado reaction. Chromatographic analysis of Trypanosoma cruzi antigens].
The aqueous extract of Trypanosoma cruzi, previously treated by benzene, was filtered through a column of Sephadex G-200. The eluted fractions were tested by complement-fixation with the chagasic reference serum. It was found that the CF antigens were eluted in the first fractions and the contaminants in the last ones. The aqueous antigen, when dried and extracted with methanol, showed a very high titer in the complement-fixation quantitative test. This antigen is free of contaminants and can be recommended for use in the serological test for the diagnosis of Chagas' disease.
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology / Q000302:isolation & purification*; D014355:Chagas Disease / Q000175:diagnosis; D002850:Chromatography, Gel; D003168:Complement Fixation Tests; D006801:Humans; D007106:Immune Sera; D014349:Trypanosoma cruzi / Q000276:immunology*
D000953:Antigens, Protozoan; D007106:Immune Sera
Estudos sobre a reacão de Guerreiro Machado. Análise cromatográfica de antígenos de Trypanosma cruzi.