Epoxygenation of arachidonic acid by rat anterior pituitary microsomal fractions.
Microsomal fractions isolated from rat anterior pituitary glands catalyze the oxygenation of arachidonic acid. By a combination of chromatographic and mass spectrometric techniques, we have identified epoxyeicosatrienoic acids as reaction products and thus documented the presence of an NADPH-dependent arachidonic acid epoxygenase activity in rat adenohypophysis.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D000090762:Cytochrome P-450 CYP2J2; D003577:Cytochrome P-450 Enzyme System; D008401:Gas Chromatography-Mass Spectrometry; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D009249:NADP / Q000494:pharmacology; D010105:Oxygenases / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000201:enzymology*; D011453:Prostaglandins / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D011453:Prostaglandins; D016718:Arachidonic Acid; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; D000090762:Cytochrome P-450 CYP2J2