Inhibition of glucosamine-6-phosphate synthetase from bacteria by anticapsin.
On the basis of kinetic studies on glucosamine-6-phosphate synthetase (EC 5.3.1.19) from six bacteria sources it has been shown that the epoxyamino acid anticapsin, a glutamine analog, is a competitive inhibitor of the enzyme in regard to glutamine with Km value of 10(-4) M and Ki varying from 10(-7) to 10(-6) M. Unlike other glutamine analogs like 6-diazo-5-oxo-L-norleucine, chloropentanoic acid, L-alpha-amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid or albizziin, anticapsin is not generally inhibitory to various amidotransferases. It does not inhibit xanthosine 5'-monophosphate amidotransferase, glutaminase or gamma-glutamyltranspeptidase.
D000409:Alanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001173:Arthrobacter / Q000201:enzymology; D001413:Bacillus thuringiensis / Q000201:enzymology; D001419:Bacteria / Q000201:enzymology*; D001667:Binding, Competitive; D002238:Carbohydrate Epimerases / Q000037:antagonists & inhibitors*; D003352:Corynebacterium / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing) / Q000037:antagonists & inhibitors*; D007700:Kinetics; D011550:Pseudomonas aeruginosa / Q000201:enzymology; D012475:Salmonella / Q000201:enzymology
C000097:anticapsin; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing); D002238:Carbohydrate Epimerases; D000409:Alanine