Sister chromatid exchange distribution in various human tissues exposed to MMC and BrdU.
The effect of two known mutagens on different human tissues was examined in an attempt to determine if tissue specific responses exist in SCE distribution on chromosome. The tissues included human lymphocytes, skin fibroblasts, ovarian and testicular cells. All cell types were exposed to varying concentrations of 5-bromodeoxyuridine (BrdU), and mitomycin C (MMC). The numbers of SCEs were recorded from each tissue. Results indicated that certain of the tissues tested appeared more sensitive to particular agents. Results also showed that in all the tissues tested the larger chromosomes in groups A to C had greater numbers of SCEs than did the smaller chromosomes in groups D to G. There were very few SCEs in F and G group chromosomes including Y.
D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002478:Cells, Cultured; D003434:Crossing Over, Genetic / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008297:Male; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology*; D009153:Mutagens / Q000494:pharmacology*; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D008937:Mitomycins; D009153:Mutagens; D016685:Mitomycin; D001973:Bromodeoxyuridine