The primary structure of mu-chain-disease protein BOT. Peculiar amino-acid sequence of the N-terminal 42 positions.
The complete primary structure of the mu heavy-chain disease (mu-HCD) protein BOT has been determined. The monomeric HCD-mu-chain consists of 391 amino-acid residues, lacking the VH and mu CH1 domains but including the entire CH2, CH3 and CH4 domains (349 residues). The sequence of the preceding 42 N-terminal residues which we designate as the "pre-C-part" presents no homology to any known variable or constant immunoglobulin sequence, but contains an internal homology of positions 10-19 to positions 20-29. The origin of the "pre-C-part" structure and the deletion of the mu CH1 domain of protein BOT are discussed.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002918:Chymotrypsin; D003488:Cyanogen Bromide; D006362:Heavy Chain Disease / Q000097:blood*; D006801:Humans; D006868:Hydrolysis; D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains; D007148:Immunoglobulin mu-Chains; D010446:Peptide Fragments / Q000032:analysis; D014357:Trypsin
D000596:Amino Acids; C041229:IGHM protein, human; D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains; D007148:Immunoglobulin mu-Chains; D010446:Peptide Fragments; D002918:Chymotrypsin; D014357:Trypsin; D003488:Cyanogen Bromide