title: Regulation of transcription of the Escherichia coli phosphoenolpyruvate carboxykinase locus: studies with pck-lacZ operon fusions.
abstract: Mutants of Escherichia coli containing genetic fusions of lacZ to the pck (phosphoenolpyruvate carboxykinase) locus were isolated by using Mu d(lacZ Ampr) bacteriophage. Synthesis of beta-galactosidase in these strains is regulated by cyclic AMP and glucose (catabolite repression). Synthesis of beta-galactosidase by pck-lacZ fusions was induced in log-phase cells growing on gluconeogenic media, was repressed by glucose, and was also induced up to 100-fold at the onset of stationary phase in LB medium. This stationary-phase induction required cyclic AMP and some other unknown regulatory signal.
mesh_terms: D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D005796:Genes; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005838:Genotype; D007700:Kinetics; D009876:Operon; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics*; D013045:Species Specificity; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D001616:beta-Galactosidase; D010729:Phosphoenolpyruvate Carboxykinase (GTP)