The standardization of the candidate national standard anti-D blood-typing serum with the Groupamatic MG50 system.
The utility of the Groupamatic MG50 system for quantitative expression of agglutination in terms of continuous response was studied. By use of the characteristics of the design of this system, in which the change in voltage reflects the degree of agglutination, a linear regression relationship between the log ratio of light flux obtained by transformation of the voltage and the log dilution factor of the serum was demonstrated. The availability of the parallel line assay method to the standardization of the blood grouping antisera was also described.
D001788:Blood Grouping and Crossmatching / Q000379:methods*; D006386:Hemagglutination Tests / Q000295:instrumentation; D006801:Humans; D007106:Immune Sera / Q000276:immunology / Q000592:standards*; D012044:Regression Analysis; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
D007106:Immune Sera; D012204:Rh-Hr Blood-Group System