title: Enzymatic synthesis of anticodon-deleted and replaced Bacillus subtilis tRNAThr and their amino acid acceptor activity.
abstract: By some enzymatic and chemical procedures, one or two nucleotides in the anticodon region of Bacillus subtilis tRNAThr were deleted and a part of the anticodon sequence was replaced. The anticodon-deleted molecules retained amino acid acceptor activity although the activity was low. However, the anticodon-replaced molecule which has a lysine specific sequence (U-U-U) exhibited neither threonine acceptor activity nor lysine acceptor activity.
mesh_terms: D000926:Anticodon; D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D011130:RNA Ligase (ATP) / Q000378:metabolism; D012343:RNA, Transfer; D012346:RNA, Transfer, Amino Acyl / Q000138:chemical synthesis / Q000235:genetics* / Q000378:metabolism; D013604:T-Phages / Q000201:enzymology; D046249:Transfer RNA Aminoacylation
chemical_list: D000926:Anticodon; D012346:RNA, Transfer, Amino Acyl; D012343:RNA, Transfer; D011130:RNA Ligase (ATP)