On-line radiochemical assay for monoamine oxidase utilizing high-performance liquid chromatography.
A fast and sensitive assay for the determination of monoamine oxidase activity was developed. The method is based on the separation and quantitation of 14C-labeled assay products by high-performance liquid chromatography, which is interfaced directly into a flow-through radioactivity detector. This allows on-line quantitation of the radioactive compounds with picomole sensitivity. The method makes possible the complete separation and detection of the deaminated products of monoamine oxidase A and B substrates benzylamine and 5-hydroxytryptamine, respectively. This assay has been applied to the measurement of monoamine oxidase A and B activities in rat brain.
D000818:Animals; D001596:Benzylamines / Q000378:metabolism; D001921:Brain / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D003010:Clorgyline / Q000494:pharmacology; D007202:Indicators and Reagents; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D008995:Monoamine Oxidase / Q000032:analysis*; D011854:Radiochemistry; D051381:Rats; D011919:Rats, Inbred Strains; D012642:Selegiline / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism
D001596:Benzylamines; D007202:Indicators and Reagents; D012642:Selegiline; D012701:Serotonin; C030796:benzylamine; D008995:Monoamine Oxidase; D003010:Clorgyline