Different polysaccharides in the external layers (capsule and slime) of the cell envelope of Rhodopseudomonas capsulata Sp11.
Two different acidic polysaccharides (I and II) were detected in the external cell envelope layers (slime and capsule) of Rhodopseudomonas capsulata Sp11. Polysaccharide I contains rhamnose, fucose, glucosamine and an unknown acidic sugar, it represents the slime material of the strain. Polysaccharide II contains rhamnose, galactose, 3-amino-3,6-dideoxygalactose, an unknown amino sugar and galacturonic acid, it represents very likely the capsule of R. capsulata Sp11. Polysaccharide I has a serological specificity different from that of polysaccharide II as shown by immunoprecipitation using antisera against living cells. Polysaccharide II, but not polysaccharide I, reacts in antiserum against heat-treated cells (100 degrees C, 2.5 h). Whole cells are agglutinated in the antisera against living but not in those against heat-treated cells.
D002462:Cell Membrane / Q000032:analysis; D002473:Cell Wall / Q000032:analysis; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D012241:Rhodopseudomonas / Q000032:analysis*; D014461:Ultracentrifugation
D011135:Polysaccharides, Bacterial