title: Effect of butylated hydroxytoluene on the disposition of [14C]aflatoxin B1 in the lactating rat.
abstract: The distribution and metabolism of an ip dose of [14C]aflatoxin B1 (AFB1) were studied in lactating Sprague-Dawley rats fed for the previous 13 days on a diet containing 0.5% butylated hydroxytoluene (BHT). Compared with ingestion of a BHT-free diet, treatment with BHT increased the biotransmission of AFB1 metabolites, predominantly aflatoxin M1 (AFM1), into the mammary gland and its content of milk, decreased AFB1 binding to liver nuclear DNA and enhanced the excretion of water-soluble metabolites of AFB1, all measured 6 hr after an oral dose of [14C]AFB1. These changes are related to the induction by BHT of hepatic enzymes involved in the transformation and detoxification of AFB1. The results suggest that exposure to BHT may protect the lactating animal from the carcinogenic effect of AFB1 but may increase the risk of exposure of the newborn infant to the carcinogenic metabolite AFM1.
mesh_terms: D016604:Aflatoxin B1; D016607:Aflatoxin M1; D000348:Aflatoxins / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D001711:Biotransformation; D002084:Butylated Hydroxytoluene / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer; D004347:Drug Interactions; D005260:Female; D008321:Mammary Glands, Animal / Q000032:analysis; D008892:Milk / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
chemical_list: D000348:Aflatoxins; D002250:Carbon Radioisotopes; D002084:Butylated Hydroxytoluene; D016607:Aflatoxin M1; D016604:Aflatoxin B1