pp60c-src is a substrate for phosphorylation when cells are stimulated to enter cycle.
The endogenous cellular oncogene products, pp60c-src, exhibits a protein kinase activity, but is itself a phosphoprotein. Based on the assumption that pp60c-src might play a role in the control of cell proliferation, we have studied its behaviour as a substrate for phosphorylation known to occur when quiescent, serum-deprived cells are stimulated to enter cell cycle following addition of either serum, platelet-derived growth factor or the phorbol ester derivative, 12-O-tetradecanoyl-phorbol-13-acetate. For this purpose a partial purification of pp60c-src on DEAE ion-exchange chromatography was combined with immune precipitation. A 2-4-fold increase in serine phosphorylation of pp60c-src was consistently observed after stimulation of quiescent cells to growth.
D000818:Animals; D001769:Blood; D002453:Cell Cycle; D002642:Chick Embryo; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism; D015688:Oncogene Protein pp60(v-src); D010766:Phosphorylation; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D012694:Serine / Q000378:metabolism; D012697:Serine Endopeptidases; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014764:Viral Proteins / Q000378:metabolism*
D010982:Platelet-Derived Growth Factor; D014764:Viral Proteins; D012694:Serine; D015688:Oncogene Protein pp60(v-src); D010450:Endopeptidases; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D013755:Tetradecanoylphorbol Acetate