title: Strain-differences and inducibility of microsomal oxidative enzymes in Drosophila melanogaster flies.
abstract: Some basic characteristics of the enzyme system involved in the oxidative metabolism of xenobiotic compounds were investigated in Drosophila melanogaster flies. Attention was focussed on (1) the normal levels of these enzymes and their activities in whole flies, in different parts of the fly's body and in different sexes, (2) the changes in levels and activities of the enzymes elicited by pretreatment of the flies with known enzyme inducers and (3) differences between strains. Four commonly used wild-type (WT) strains, three insecticide resistant strains (IR) and one white-eyed mutant strain were employed. Except in those experiments on sex differences and in spatial distribution in the fly's body of the enzymatic activities, microsomes were isolated from whole-body homogenates of mixtures of female and male flies. Microsomal cytochrome P-450, benzo[a]pyrene (BP) hydroxylation, p-nitroanisole (pNA) demethylation and aminopyrine (AP) demethylation were measured in control flies and in flies pretreated with Aroclor 1254 (AC), phenobarbital (PB) or butylated hydroxytoluene (BHT). In flies of the WT strain Berlin-K, there were no significant differences in BP hydroxylation activity and its inducibility between the two sexes. In males, inducibility of BP hydroxylation activity was similar in the head, thorax and abdomen, but significantly lower in testis. Considerable differences in some enzyme activities were found between the strains. pNA demethylation and AP demethylation were substantially higher in all IR strains, while no correlation could be found between their increased insecticide resistance and BP hydroxylating capacity or cytochrome P-450 content of the microsomes. Response to enzyme inducing compounds was found to be strain-dependent. PB proved to be a more efficient inducer of BP hydroxylation than AC, which does induce pNA demethylation. BHT has inducing properties that are intermediate between PB and AC. IR strain Hikone-R turned out to be an exception, possessing very low BP hydroxylating capacity and a low degree of inducibility of mixed-function oxidase activities. Differential temperature dependence was found for BP hydroxylation as compared with pNA demethylation. While BP hydroxylation was doubled when raising the temperature from 25 degrees C to 35 degrees C, pNA demethylation was reduced by 50%.
mesh_terms: D000633:Aminopyrine N-Demethylase / Q000096:biosynthesis; D000818:Animals; D001140:Aroclors / Q000494:pharmacology; D001579:Benzopyrene Hydroxylase / Q000096:biosynthesis; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D020111:Chlorodiphenyl (54% Chlorine); D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D004331:Drosophila melanogaster; D004790:Enzyme Induction; D005260:Female; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D009575:Nitroanisole O-Demethylase / Q000096:biosynthesis; D010634:Phenobarbital / Q000494:pharmacology; D012737:Sex Factors; D013045:Species Specificity
chemical_list: D001140:Aroclors; D020111:Chlorodiphenyl (54% Chlorine); D002084:Butylated Hydroxytoluene; D003577:Cytochrome P-450 Enzyme System; D009575:Nitroanisole O-Demethylase; D001579:Benzopyrene Hydroxylase; D000633:Aminopyrine N-Demethylase; D010634:Phenobarbital