[Study of the stimulating action of sturin, its fractions, and the internal protein of T2 phage in transfection of E. coli spheroplasts by Lambda phage DNA].
The stimulating action of sturin and its fractions in the transfection of E. coli spheroplasts with DNA of phage lambda has been shown, and the optimum conditions for the infection of sturin-treated spheroplasts with phage DNa have been established. The biological effect was most pronounced in arginine-enriched low-molecular sturin fraction B. The treatment of spheroplasts with the internal protein of phage T2 did not stimulate transfection. These findings suggest that the specificity of the stimulating action of protamine depends on the peculiarities of its amino acid composition, viz. a high content of arginine which is present in protein molecules in the form of blocks made up of 2-6 amino acid residues.
D001120:Arginine / Q000494:pharmacology; D010582:Bacteriophage lambda / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D011479:Protamines / Q000494:pharmacology*; D013104:Spheroplasts / Q000187:drug effects*; D013268:Stimulation, Chemical; D013604:T-Phages / Q000032:analysis*; D014162:Transfection / Q000187:drug effects*; D014764:Viral Proteins / Q000494:pharmacology*
D011479:Protamines; D014764:Viral Proteins; C015513:sturin; D001120:Arginine
Issledovanie stimuliruiushchego deĭstviia sturina, ego fraktsiĭ i vnutrennego belka faga T2 v transfektsii sferoplastov E. coli DNK faga lambda.