title: Growth of bacteriophage phiX-174 at elevated temperatures.
abstract: The replication of bacteriophage phiX-174 is impaired at temperatures above 40 degrees C. Mutants (ht) that replicate at high temperature were isolated and partially characterized. Wild-type phiX fails to grow at high temperature because, unlike the mutants, it does not make appreciable amounts of single-stranded (ss)DNA. An unusual form of ssDNA, not found in complete virions, is described.
mesh_terms: D010584:Bacteriophage phi X 174 / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000032:analysis / Q000096:biosynthesis; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis; D006358:Hot Temperature; D009154:Mutation
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral