Sequential determination of circulating immune complexes during progressive and regressive phases of mouse mastocytoma.
Circulating immune complexes were determined by 125I-C1q-binding and Raji cell-binding sequentially during distinct phases of progression and regression of a weakly immunogenic murine tumour. No increase in levels of circulating immune complexes was found at any time during tumour development, although reference complexes formed between tumour cell membrane antigens and a murine histocompatibility antigen-directed alloantibody were easily detected by both tests. These findings parallel the absence of humoral antibody during tumour development but are in some contradiction to the pronounced B cell proliferation which was observed in this tumour model. The results add evidence for a more critical evaluation of the role which levels of circulating immune complexes might play in tumour diagnosis and prognosis.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis*; D003166:Complement Activating Enzymes; D015922:Complement C1q; D005260:Female; D007158:Immunologic Techniques; D012515:Mast-Cell Sarcoma / Q000276:immunology*; D051379:Mice; D008811:Mice, Inbred DBA; D012015:Reference Standards; D013997:Time Factors
D000936:Antigen-Antibody Complex; D015922:Complement C1q; D003166:Complement Activating Enzymes