Characteristics of mitochondrial and synaptosomal monoamine oxidase in monkey brain.
Enzymic properties of monoamine oxidase (MAO) from monkey brain were studied. High MAO activity was observed in the mesencephalon and dienecephalon of the brain. Highest activity in every region of the brain was found with tyramine as a substrate. Monkey brain mitochondrial MAO showed a different substrate specificity and different Km and Vmax values than the enzyme from mice, rats, guinea pigs and rabbits. The pH activity curves were all bell-shaped, but the pH optima were remarkably different with the various substrates used. The activities of various substrates at pH 7.2 were compared with those at the pH optimum. At the pH optima, the activity was about 1.2-fold higher with tyramine and dopamine, 2-fold higher with beta-phenylethylamine (beta-PEA) and 3-fold higher with serotonin (5-HT) and benzylamine. These results were almost similar when synaptosomes from monkey brain were used. MAO activities with 5-HT and beta-PEA were strongly inhibited by much lower concentrations of clorgyline and deprenyl, respectively. Plateau-shaped inhibition curves by these inhibitors were obtained with tyramine as the substrate. These results indicate that both the A- and B-form of MAO appear to be uniformly distributed in monkey brain, and the A-form of MAO represents approximately 35% and 50% of the total MAO activity in mitochondria and synaptosomes, respectively.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002417:Cattle; D003010:Clorgyline / Q000494:pharmacology; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008251:Macaca; D051379:Mice; D008928:Mitochondria / Q000201:enzymology*; D008995:Monoamine Oxidase / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010627:Phenethylamines / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D012642:Selegiline / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology; D013045:Species Specificity; D013379:Substrate Specificity; D013574:Synaptosomes / Q000201:enzymology*; D014439:Tyramine / Q000494:pharmacology
D009419:Nerve Tissue Proteins; D010627:Phenethylamines; D012642:Selegiline; D012701:Serotonin; D008995:Monoamine Oxidase; D003010:Clorgyline; D014439:Tyramine