Arachidonic acid metabolism by the non-pregnant human uterus.
Arachidonic acid metabolism in the non-pregnant human uterus was studied using short term incubations of human endometrium and myometrium with [14C] arachidonic acid followed by HPLC separation of the arachidonic acid metabolites formed. Lipoxygenase as well as cyclooxygenase activity was found in both the endometrium and myometrium although the endometrium predominated in arachidonic acid product formation. The lipoxygenase products formed by these tissues included the monohydroxy acids 5-HETE and 12-HETE.
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000328:Adult; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000096:biosynthesis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D046508:Culture Techniques; D015237:Dinoprost; D015232:Dinoprostone; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006801:Humans; D008084:Lipoxygenase / Q000378:metabolism; D008875:Middle Aged; D009215:Myometrium / Q000378:metabolism*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011458:Prostaglandins E / Q000096:biosynthesis; D011460:Prostaglandins F / Q000096:biosynthesis
D001095:Arachidonic Acids; D011458:Prostaglandins E; D011460:Prostaglandins F; D016718:Arachidonic Acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D015237:Dinoprost; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone