Antibodies to human c-myc oncogene product: evidence of an evolutionarily conserved protein induced during cell proliferation.
Antisera to a synthetic c-myc peptide and to c-myc antigens synthesized from various portions of the human gene expressed in Escherichia coli were used in order to characterize the protein product of the human c-myc oncogene. Although the deduced molecular weight of the human c-myc protein is 49,000, these antisera precipitate a protein from human cells that migrates in sodium dodecyl sulfate-polyacrylamide gel as if its molecular weight were 65,000. In addition, the mouse c-myc protein, whether synthesized in cells or in a cell-free system directed by pure, synthetic messenger RNA, has analogous properties and is immunoprecipitated by the antiserum to the human c-myc protein. Similar proteins are immunoprecipitated from monkey, rat, hamster, and frog cells, suggesting evolutionary conservation of antigenic structure of the c-myc protein among vertebrates. In addition, and in a manner consistent with the behavior of its messenger RNA, the immunoprecipitable c-myc protein is sharply induced by the action of mitogens on resting human T cells.
D000595:Amino Acid Sequence; D000818:Animals; D000912:Antibodies, Neoplasm / Q000276:immunology*; D001483:Base Sequence; D002455:Cell Division; D002645:Chickens; D006224:Cricetinae; D004273:DNA, Neoplasm / Q000235:genetics; D004274:DNA, Recombinant / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D000882:Haplorhini; D006801:Humans; D051379:Mice; D008934:Mitogens / Q000494:pharmacology; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000235:genetics / Q000276:immunology*; D009857:Oncogenes; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D051381:Rats
D000912:Antibodies, Neoplasm; D004273:DNA, Neoplasm; D004274:DNA, Recombinant; D008934:Mitogens; D009363:Neoplasm Proteins; D012333:RNA, Messenger