Quantitative determination of immunoglobulins in the cerebrospinal fluid based on their light chain properties using a solid-phase enzyme immunoassay.
A method for quantitative determination of immunoglobulins in the cerebrospinal fluid (CSF) on the basis of their light chain properties is described. The method is a solid-phase enzyme immunoassay, based upon competitive binding of immunoglobulins in unknown and labeled immunoglobulin for antibody adsorbed to the wells of a microplate. The values for immunoglobulins kappa (Ig-k), lambda (Ig-l) and k/l ratio obtained from 38 subjects without neurological disorders were 8.6 +/- 4.0 micrograms/ml, 7.0 +/- 2.6 micrograms/ml and 1.21 +/- 0.21, respectively (mean +/- SD). Our method is considered to be less influenced by the molecular size and weight of immunoglobulins than radial immunodiffusion, and thus may provide more precise values of Ig-k and Ig-l.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001667:Binding, Competitive; D004800:Enzymes, Immobilized; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000134:cerebrospinal fluid; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid; D007147:Immunoglobulin Light Chains / Q000134:cerebrospinal fluid*; D007075:Immunoglobulin M / Q000134:cerebrospinal fluid; D007145:Immunoglobulin kappa-Chains / Q000134:cerebrospinal fluid; D007146:Immunoglobulin lambda-Chains / Q000134:cerebrospinal fluid; D007136:Immunoglobulins / Q000134:cerebrospinal fluid*; D008297:Male; D008875:Middle Aged
D004800:Enzymes, Immobilized; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D007136:Immunoglobulins