Synthesis of dinitrophenyl derivatives of 3-O-fatty acyl esters of serine and threonine.
The synthesis of 2,4-dinitrophenyl derivatives of the 3-O-oleoyl and 3-O-palmitoyl esters of serine and threonine are described. The derivatives were purified by preparative thin-layer chromatography (TLC) and characterized by 1H-nuclear magnetic resonance (NMR) spectroscopy. These derivatives may be useful for researchers interested in characterizing covalently bound fatty acids on serine and threonine hydroxyl groups of cellular proteins.
D000818:Animals; D002855:Chromatography, Thin Layer; D004140:Dinitrophenols / Q000138:chemical synthesis; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009829:Oleic Acids / Q000138:chemical synthesis*; D010169:Palmitic Acids / Q000138:chemical synthesis*; D012694:Serine / Q000138:chemical synthesis*; D013912:Threonine / Q000138:chemical synthesis*
D004140:Dinitrophenols; D007202:Indicators and Reagents; D009829:Oleic Acids; D010169:Palmitic Acids; D013912:Threonine; D012694:Serine