Inverted terminal repetition in vaccinia virus DNA encodes early mRNAs.
Vaccinia virus DNA contains a long inverted terminal repetition of MW approximately 6.8 x 10(6). A fragment of MW 6.3 X 10(6) from this repetition has been cloned in coliphage lambda and used to isolate RNA from virus-infected cells. Electron microscopy indicates that early RNAs are transcribed from the repeated sequence and cell-free translation shows that the RNAs code for polypeptides.
D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D014158:Transcription, Genetic; D014615:Vaccinia / Q000378:metabolism; D014616:Vaccinia virus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
D004274:DNA, Recombinant; D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins