[Effect of hypothermia on subcellular distribution and various physico-chemical properties of cathepsin D in rat brain].
The total cooling ef rats down to the rectal temperature 30 degrees and 20 degrees C does not change significantly the ratio of the relative specific activity of cathepsin D in subcellular fractions of the rat brain. The gel chromatographic analysis of heterogeneity of cathepsin D molecular forms in subcellular fractions established the presence of a high-molecular (in the fractions of lysosome and microsome mitochondria) and a low-molecular (in the fractions of lysosome and cytosol mitochondria) enzyme forms. Under hypothermia (20 degrees C) in the brain cytosol fraction there arises a minor zone of the cathepsin D activity corresponding to the high-molecular enzyme form.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002402:Cathepsin D; D002403:Cathepsins / Q000032:analysis*; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000201:enzymology; D002850:Chromatography, Gel; D003080:Cold Temperature; D003600:Cytosol / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology; D008861:Microsomes / Q000201:enzymology; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains
D002403:Cathepsins; D002402:Cathepsin D
Vliianie gipotermii na subkletochnoe raspredelenie i nekotorye fiziko-khimicheskie svoĭstva katepsina D v golovnom mozgu krys.