[Purification of neuraminidase from influenza virus on an immunosorbent].
A procedure for isolation of neuraminidase from influenza virus using the nonionic detergent Triton x-100 was developed. To achieve further purification, the protein mixture was passed through a Sepharose column packed with immobilized antibodies against hemagglutinin. The neuraminidase preparation thus obtained fully retained its enzymatic and antigenic properties and during electrophoretic separation under denaturating conditions gave one protein band.
D000818:Animals; D002642:Chick Embryo; D005779:Immunodiffusion; D007164:Immunosorbents; D009980:Influenza A virus / Q000201:enzymology*; D009439:Neuraminidase / Q000302:isolation & purification*; D012995:Solubility
D007164:Immunosorbents; D009439:Neuraminidase
Ochistka neraminidazy virusa grippa na immunosorbente.