title: Effect of glycosidases on the properties of human interferon gamma.
abstract: Heterogeneity of human gamma interferon (IFN-gamma) induced by the combined treatment with OK-432 and Staphylococcal enterotoxin B (SEB) was demonstrated by chromatofocusing. Treatment of IFN-gamma with a mixture of neuraminidase, and beta-galactosidase eliminated the charge heterogeneity. Apparent molecular weight of IFN-gamma was decreased by enzyme treatment. These results suggest that the heterogeneity of IFN-gamma induced in our system was the result of the difference in the content of sialic acids.
mesh_terms: D006026:Glycoside Hydrolases / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D007525:Isoelectric Focusing; D008970:Molecular Weight; D009439:Neuraminidase / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D007371:Interferon-gamma; D006026:Glycoside Hydrolases; D009439:Neuraminidase; D001616:beta-Galactosidase