[Adsorption of lambda phage DNA onto Escherichia coli cells treated with Ca2+ ions and onto frozen--thawed bacteria].
The study of the biologically active tritium-labeled phage lambda DNA adosrption on Ca2+-treated and frozen--thawed E. coli cells showed the absence of a correlation between the adsorption level and transfection efficiency. Thus the infectious phage lambda DNA adsorption level does not change, while frozing--thawing of E. coli cells but it increases ten-fold when treating the cells with Ca2+ in ice, the transfection efficiency level with this DNA being equal for both types of recipients.
D000327:Adsorption; D010582:Bacteriophage lambda / Q000235:genetics*; D002118:Calcium / Q000494:pharmacology*; D004279:DNA, Viral; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005615:Freezing; D014162:Transfection
D004279:DNA, Viral; D002118:Calcium
Adsorbtsiia DNK faga lambda na kletkakh Escherichia coli, obrabotannykh ionami Ca2+, i na zamorozhennykh-ottaiannykh bakteriiakh.