title: Characterization of natural killer cells induced in the peritoneal exudates of mice infected with Listeria monocytogenes: a study of their tumor target specificity and their expression of murine differentiation antigens and human NK-associated antigens.
abstract: That M1/70, a monoclonal anti-murine macrophage antibody, recognizes murine natural killer cells (NK) and that there is an increase in NK following intraperitoneal infection with live Listeria monocytogenes (LM) was previously reported. Here, LM-induced NK cells were further characterized with respect to tumor target specificity and the expression of murine mast cell, mononuclear phagocyte, and lymphocyte differentiation antigens plus human NK-associated antigens. The M1/70-selected NK (Mac 1 NK) lysed Yac 1, RLmale 1, and WEHI 164.1, but not EL 4 or WTS cells. Immunoprecipitation with M1/70 demonstrated that Mac 1, the antigen recognized by M1/70, was present on NK and thioglycollate-elicited macrophages. Contaminating macrophages in the NK-enriched population did not account for the immunoprecipitated Mac 1. Mac 1 NK that lysed Yac 1 displayed Qa 5, LFA 1, asialo GM 1, Ly 5.1, and NK 1.2, but not Lyt 1, Lyt 2, Mac 2, Mac 3, or Mac 4. Thirty percent of these Mac 1 NK bore Thy 1.2. The presence of Thy 1.2 did not correlate with individual lytic efficiency or cell cycle. Antibodies to human NK antigens Leu 7, Leu 11a, and Leu 15 did not recognize LM-induced NK cells.
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002454:Cell Differentiation; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007113:Immunity, Innate; D007694:Killer Cells, Natural / Q000145:classification / Q000276:immunology*; D007942:Leukemia, Experimental / Q000276:immunology; D008089:Listeria monocytogenes; D008088:Listeriosis / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains
chemical_list: D000954:Antigens, Surface