Effect of Japanese seaweed (Laminaria angustata) extracts on the mutagenicity of 7,12-dimethylbenz[a]anthracene, a breast carcinogen, and of 3,2'-dimethyl-4-aminobiphenyl, a colon and breast carcinogen.
Animal model studies suggest that diets containing Laminaria angustata, a brown seaweed commonly eaten in Japan, inhibit breast carcinogenesis. In order to identify the compound(s) in the seaweed responsible for tumor-inhibiting activity, we used Ames/mammalian microsome assay system to determine the antimutagenic (or anticarcinogenic) effect of various solvents and water extracts of Laminaria angustata. The antimutagenic effects of acetone, ether, chloroform, chloroform + methanol, hot water and cold water extracts on the mutagenicity induced by 7,12-dimethylbenz[a]anthracene (DMBA), a breast carcinogen, and 3,2'-dimethyl-4-aminobiphenyl (DMAB), a colon and breast carcinogen, was studied using the Salmonella typhimurium strains TA98 and TA100. All extracts were nonmutagenic in both bacterial tester strains. The addition of 10-100 mg solvent extracts of seaweed/plate greatly inhibited DMAB-induced mutagenicity in both tester strains (80-96% inhibition) and DMBA-induced mutagenicity in TA100 (about 82%), whereas hot and cold water extracts produced a moderate inhibition in a dose-related manner in both strains.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000037:antagonists & inhibitors*; D000611:Aminobiphenyl Compounds; D000814:Aniline Compounds / Q000037:antagonists & inhibitors*; D001551:Benz(a)Anthracenes / Q000037:antagonists & inhibitors*; D002273:Carcinogens / Q000037:antagonists & inhibitors; D004032:Diet; D004159:Diphenylamine / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors*; D007795:Laminaria; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects*; D012486:Salmonella typhimurium / Q000187:drug effects; D012624:Seaweed
D000611:Aminobiphenyl Compounds; D000814:Aniline Compounds; D001551:Benz(a)Anthracenes; D002273:Carcinogens; C001042:2',3-dimethyl-4-aminobiphenyl; D015127:9,10-Dimethyl-1,2-benzanthracene; D004159:Diphenylamine