Synthesis and maturation of lambda receptor in Escherichia coli K-12: in vivo and in vitro expression of gene lamB under lac promoter control.
The lambda receptor is an outer membrane protein from Escherichia coli K-12 lamB, its structural gene, is part of the maltose regulon. We have cloned this gene in a phage so that it is under the control of the lac promoter. The phage was devised in such a way that it can infect lamB mutants and that chromosomal lamB mutations can be transferred to it. In vivo, the lambda receptor is expressed under lac promoter control and is exported normally to the outer membrane, independently of the expression of the other genes of the maltose regulon. In vitro, DNA of the phage allows efficient synthesis of the lamB product. The protein--or pre-lambda-receptor--made in vitro contains an NH2-terminal sequence of about 25 amino acids not found in the lambda receptor. We have detected no inactivation of phage lambda by the pre-lambda-receptor. Conversion of the pre-lambda-receptor to a form that has the apparent molecular weight of the mature lambda receptor was achieved. A lamB mutation that blocks export in vivo also blocks conversion in vitro.
D001426:Bacterial Proteins / Q000096:biosynthesis; D010582:Bacteriophage lambda / Q000378:metabolism*; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000378:metabolism*; D007763:Lac Operon; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D009876:Operon; D011498:Protein Precursors / Q000378:metabolism; D011991:Receptors, Virus / Q000096:biosynthesis* / Q000235:genetics
D001426:Bacterial Proteins; D004274:DNA, Recombinant; D008565:Membrane Proteins; D011498:Protein Precursors; D011991:Receptors, Virus