Evidence of RNA in D loops of intracellular lambda DNA.
If lambda DNA replication is blocked by mutation in any one of several genes essential for replication, intracellular lambda DNA often shows short three-stranded regions called D loops. In this report we show that one arm of a D loop is an RNA . DNA hybrid, whereas the remaining arm is made up of single-stranded DNA. The RNA can be partially removed by RNase A and totally removed by RNase H. Also, D loops do not appear if infections are made in cells treated with rifampin, a potent inhibitor of transcription by Escherichia coli RNA polymerase. Several genes associated with recombination, including the host recA gene, are not essential for D-loop formation.
D010582:Bacteriophage lambda / Q000235:genetics*; D002874:Chromosome Mapping; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000378:metabolism*; D011995:Recombination, Genetic; D012293:Rifampin / Q000494:pharmacology; D014779:Virus Replication
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D012367:RNA, Viral; D012293:Rifampin