[Inhibition by antibiotics of the incorporation of labelled amino acids into the nuclear matrix proteins of the Zajdela hepatoma].
The incorporation of radioactivity into nuclear matrix proteins during incubation of Zajdela hepatoma cells with labelled amino acids was strongly inhibited by chloramphenicol and cycloheximide and slightly inhibited by actinomycin D and mitomycin C. The antibiotics studied inhibited the incorporation of the radioactive label preferentially into proteins with Mr 150 000-220 000, approximately 55 000 and less than 26 000. During incubation of ascites tumour cells with antibiotics, predominantly with chloramphenicol, a decrease in the content of some protein components was observed as well. As in the low molecular weight protein fraction, the intense inhibition of the radioactive label and a decrease of its content was observed, a conclusion is drawn that this protein fraction is characterized by a high turnover rate.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism*; D002701:Chloramphenicol / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000378:metabolism*; D051381:Rats
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D008937:Mitomycins; D009363:Neoplasm Proteins; D003609:Dactinomycin; D016685:Mitomycin; D002701:Chloramphenicol
Podavlenie nekotorymi antibiotikami vkliucheniia mechenykh amminokislot v belki iadernogo matriksa gepatomy Zaĭdelia.