The effect of solubilized bone matrix fractions from different mammalian species on glycosaminoglycan synthesis by cultured fibroblasts.
Human and bovine bone matrices were extracted with salt solutions of different composition and the extracts tested for stimulation of incorporation of radioactivity from [3H]glucosamine and [35S]SO4 into the hyaluronic acid and chondroitin sulfate of the cell pellet, the cell surface and the medium fractions of human synovial cells in culture. Stimulatory activity was extracted with a solution of 0.3 M EDTA in 2.5 M NaCl from bovine but not human bone. Subsequent extraction of the residues with 4 M guanidinium hydrochloride yielded activity from both matrices. A major stimulation of incorporation of radioactivity was observed in the cell surface fractions. Human synovial cells constitute a more sensitive assay system for the stimulatory activity than rabbit synovial cells.
D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D002417:Cattle; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis; D007700:Kinetics; D051381:Rats; D012995:Solubility; D013045:Species Specificity; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes; D013583:Synovial Membrane / Q000378:metabolism*; D014020:Tissue Extracts / Q000494:pharmacology*; D014316:Tritium
D006025:Glycosaminoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes; D014020:Tissue Extracts; D014316:Tritium; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D005944:Glucosamine