title: [Allelic exclusion in hybridomas. I. Isolation and properties of a hybrid clone producing two allelic variants of light chain immunoglobulins in the rat].
abstract: Expression of RI-1a and RI-1b allelic genes controlling the production of rat lg kappa L-chains by hybridoma cells in vitro was studied. By fusing mouse myeloma cells with RI-1a/RI-1b heterozygous rat splenocytes the unique cloned hybrid cell line secreting both allelic variants has been established. This line may have appeared because cell hybridization made it possible to fix the rare case of correct rearrangement of the kappa chain gene segments on both homologous chromosomes.
mesh_terms: D000483:Alleles; D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D005786:Gene Expression Regulation; D006825:Hybridomas / Q000276:immunology*; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D009101:Multiple Myeloma / Q000276:immunology / Q000473:pathology; D010641:Phenotype; D051381:Rats; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains
vernacular_title: Allel'noe iskliuchenie v gibridomakh. I. Poluchenie i svoĭstva gibridomnogo klona, produtsiruiushchego dva allel'nykh varianta legkikh tsepeĭ immunoglobulinov krysy.