[The use of immunological method for the determination of acid alpha-glucosidase activity in human muscles].
Homogeneous preparation of acid alpha-glucosidase, obtained from human placenta, was used for immunization of rabbits and production of specific antiserum. Immunological procedure was developed for estimation of acid alpha-glucosidase activity from human muscles in presence of the enzyme neutral form. Activity of acid alpha-glucosidase was measured in 20 patients with various types of nervous system impairments. Total activity of alpha-glucosidase in muscles of the patients was 11.4 +/- 0.7 nM/min/g of the tissue and activity of the acid form constituted about 84.8 +/- 1.7% of the total activity.
D004589:Electrophoresis, Disc; D005959:Glucosidases / Q000032:analysis*; D006801:Humans; D007106:Immune Sera / Q000032:analysis; D005779:Immunodiffusion; D009132:Muscles / Q000201:enzymology*; D009422:Nervous System Diseases / Q000201:enzymology; D000520:alpha-Glucosidases / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification
D007106:Immune Sera; D005959:Glucosidases; D000520:alpha-Glucosidases
Ispol'zovanie immunologicheskogo metoda dlia opredeleniia aktivnosti kisloĭ alpha-gliukozidazy v myshtsakh cheloveka.