title: 2-[8-14C]naphthyl 2-diazo-3,3,3-trifluoropropionate, a new carbene generating reagent for probing hydrophobic membrane domains.
abstract: A new precursor of a lipophilic photolabel, 2-[8-14C]naphthyl 2-diazo-3,3,3-trifluoropropionate (NADIT) has been synthesized. The suitability of the reagent for labeling the hydrophobic core of membranes is demonstrated by studying its reactivity in chromatophores of Rhodospirillum rubrum G-9+. The label binds preferentially to the phospholipids and intrinsic membrane proteins. In isolated reaction centers treated with NADIT the hydrophobic subunits M and L are more labeled than the H subunit. The high reactivity, dark stability and ease of synthesis favors this very lipophilic reagent to identify the intrinsic hydrophobic sections of membrane proteins.
mesh_terms: D000345:Affinity Labels / Q000138:chemical synthesis*; D020130:Bacterial Chromatophores / Q000032:analysis; D003979:Diazonium Compounds / Q000138:chemical synthesis*; D013058:Mass Spectrometry; D008563:Membrane Lipids / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis; D010777:Photochemistry; D012247:Rhodospirillum rubrum / Q000032:analysis
chemical_list: D000345:Affinity Labels; D003979:Diazonium Compounds; D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids; C040966:2-naphthyl 2-diazo-3,3,3-trifluoropropionate