Release of labile cyclo-oxygenase products of arachidonic acid from kidney by endotoxin.
The possible release of prostaglandin (PG)-like substances was studied in isolated perfused kidneys from intact and from intrarenal endotoxin (Lipopolysaccharide-LPS)-injected rabbits, using the venous outflow superflow superfuse assay organ technique. Injection of LPS into the renal artery of an LPS-pretreated kidney caused a release of thromboxane A2 (TXA2) and prostacyclin (PGI2)-like materials into the venous effluent as verified by the responses of the specific assay organs. No detectable release of these substances was found in the venous outflow of LPS-injected intact kidney. The possible role of labile cyclo-oxygenase products of arachidonic acid in the Shwartzman reaction is discussed.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001681:Biological Assay; D011464:Epoprostenol / Q000378:metabolism* / Q000494:pharmacology; D004926:Escherichia coli; D005260:Female; D007668:Kidney / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D011312:Pressure; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D013928:Thromboxane A2 / Q000378:metabolism* / Q000494:pharmacology; D013931:Thromboxanes / Q000378:metabolism*
D001095:Arachidonic Acids; D008070:Lipopolysaccharides; D013931:Thromboxanes; D016718:Arachidonic Acid; D013928:Thromboxane A2; D011464:Epoprostenol; D011451:Prostaglandin-Endoperoxide Synthases