Effect of low molecular factors released during allogeneic interactions of leucocytes or brain cells on PHA- and LPS-induced DNA synthesis in lymphocytes.
Male (A/Ph and C3H/Cbi/BOM) mice were used in our experiments. The cell-free supernatants of spleen mixed leucocyte cultures and allogeneic brain cortex cells were collected upon short-term incubation and tested in a comparative study. Spleen and brain low molecular factors were fractionated (ultrafiltration and gel column chromatography) and assayed for their activity in mitogen-induced 3H-thymidine incorporation into spleen lymphocytes. The specific release of the allogeneic spleen fraction (ASF-2) has been observed. A non-specific stimulating effect of ASF-2 on PHA- and LPS-activated spleen lymphocytes was shown. In contrast, a non-specific suppressive effect of brain fraction on PHA- and LPS-stimulated lymphocytes was demonstrated. The opposite effects of spleen and brain factors can be connected with their different composition. The lymphocyte cultures were not affected by spleen or brain factor, when cultured without the addition of mitogen. Positive and negative regulation or modulation of triggering events in lymphocyte activation by these factors can be suggested.
D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology*; D002850:Chromatography, Gel; D004247:DNA / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D051379:Mice; D008970:Molecular Weight; D010835:Phytohemagglutinins / Q000494:pharmacology
D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D004247:DNA