[Technic of radioactive labeling of autologous human thrombocytes using 111-indium-oxine and 111-indium-oxine-sulfate and their clinical use].
Using 111indium oxine and 111indium oxine sulphate as platelet labels, maximal labelling efficiency can be achieved already after 3 minutes at an incubation temperature of 37 degrees C. Mean labelling efficiency values of about 90% are reached at a platelet count of greater than 10(9) platelets/ml, but the labelling efficiency is satisfactory also at a rather low platelet count of about 10(6) platelets/ml (71% on average). Platelet labelling with these tracers allows the calculation of platelet half-life in vivo, and gamma-camera imaging of platelet aggregates in thrombosis and renal transplant rejection as well. In the present study, the platelet half-life is significantly shortened in patients with coronary heart disease (n = 15), peripheral vascular disease (n = 13) and primary hyperlipoproteinaemia (n = 32) in comparison with 106 controls. No age dependence of platelet half-life was found in the different groups of patients whereas in the control group, a significant negative correlation between age and platelet half-life was observed.
D000328:Adult; D000367:Age Factors; D000368:Aged; D001161:Arteriosclerosis / Q000000981:diagnostic imaging; D001792:Blood Platelets / Q000000981:diagnostic imaging*; D003327:Coronary Disease / Q000000981:diagnostic imaging; D006207:Half-Life; D006801:Humans; D006912:Hydroxyquinolines; D006951:Hyperlipoproteinemias / Q000000981:diagnostic imaging; D007204:Indium; D007553:Isotope Labeling; D008722:Methods; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D009942:Organometallic Compounds; D015125:Oxyquinoline / Q000031:analogs & derivatives; D011877:Radionuclide Imaging
D006912:Hydroxyquinolines; D009942:Organometallic Compounds; D007204:Indium; C032950:indium oxine; D015125:Oxyquinoline
Technik der radioaktiven Markierung autologer menschlicher Thrombozyten mit 111-Indium-Oxin und 111-Indium-Oxin-Sulfat und deren klinische Anwendung.