An easy method for the selection of restriction- and modification-deficient mutants of Escherichia coli K-12.
An easy and rapid method for selecting restriction- and modification-defective mutants of Escherichia coli K-12 is described. This method employs selection of tetracycline resistant lysogens after infection with lambda::Tn10 phage and results in a high yield of spontaneous rk-mk- and rk-mk+ mutants.
D001431:Bacteriological Techniques; D010582:Bacteriophage lambda / Q000235:genetics; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification*; D005821:Genetic Techniques; D008242:Lysogeny; D009154:Mutation; D010641:Phenotype; D013752:Tetracycline / Q000494:pharmacology
D013752:Tetracycline