Flow microfluorometric analysis of cellular DNA: Critical comparison of mithramycin and propidium iodide.
Mithramycin and propidium iodide were used to stain HeLa cells, human lymphoma cells, and phytohemagglutinin-stimulated lymphocytes for flow microfluorometric analysis of cellular DNA. The stains provided similar estimates for the proliferative fraction of the populations. However, significant differences in the relative fluorescent intensity were demonstrated in the three cell populations. Fluorescent intensity of HeLa and lymphoma cells stained with mithramycin was higher than matched propidium iodide-stained cells. Normal lymphocytes showed greater fluorescent intensity when stained with propidium iodide. Differences in the staining behavior of these two dyes may prove to be highly informative probes of chromatin structural differences.
D000818:Animals; D002645:Chickens; D004247:DNA / Q000032:analysis*; D005470:Fluorometry; D006367:HeLa Cells / Q000032:analysis; D006651:Histocytochemistry; D006801:Humans; D008214:Lymphocytes / Q000032:analysis / Q000187:drug effects; D008223:Lymphoma / Q000032:analysis; D010617:Phenanthridines; D010835:Phytohemagglutinins / Q000494:pharmacology; D008926:Plicamycin; D011419:Propidium
D010617:Phenanthridines; D010835:Phytohemagglutinins; D011419:Propidium; D004247:DNA; D008926:Plicamycin