Attempts to establish the scrapie agent in cell lines.
Attempts were made to establish a persistent infection with scrapie agent in four murine cell lines. Of the four lines tested, viz. P388D1, P3-NS1-Ag-1 (NS1), L cells and an NS1 spleen cell hybrid, only the NS1 cell line showed any evidence of agent replication. Ten per cent dimethylsulphoxide (DMSO) was included in the culture media of L cells inoculated with scrapie agent. This treatment raised the initial levels of scrapie agent associating with the L cells but did not result in a persistently infected cell line. An effect of DMSO in the inoculum was observed in mice inoculated intraperitoneally, the incubation period of the disease was considerably shortened.
D000818:Animals; D002460:Cell Line; D003840:Deoxycholic Acid / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D006822:Hybrid Cells; D007739:L Cells / Q000187:drug effects; D008221:Lymphoid Tissue; D008748:Methylcholanthrene; D051379:Mice; D009374:Neoplasms, Experimental / Q000139:chemically induced; D011328:Prions / Q000187:drug effects / Q000502:physiology*; D013154:Spleen; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication / Q000187:drug effects
D011328:Prions; D003840:Deoxycholic Acid; D008748:Methylcholanthrene; D004121:Dimethyl Sulfoxide