Prevention of suppressed interferon gamma production in thermally injured mice by administration of a novel organogermanium compound, Ge-132.
As reported previously, gamma interferon (IFN-gamma) production was selectively decreased in thermally injured mice (TI-Mice) and spleen cell cultures from the mice following stimulation with various IFN inducers. The decrease in IFN production was associated with splenic suppressor macrophages. The present study demonstrated that TI-Mice treated with Ge-132 (TGe-Mice) produced IFN following stimulation with IFN-gamma inducers. The level of IFN activity detected in the sera of TGe-Mice approximated that of controls. Similar results were obtained when spleen cells prepared from TGe-Mice were stimulated with IFN-gamma inducers in vitro. While transfer of spleen cells from TI-Mice resulted in the suppression of IFN production in normal mice (N-Mice) stimulated with IFN-gamma inducers, the transfer of spleen cells derived from TGe-Mice did not induce suppression of IFN production in N-Mice. Mononuclear cells (MNC) prepared from N-Mice produced IFN following concanavalin A (Con A) stimulation when they were co-cultured with macrophage-enriched populations (PAC) obtained from the spleens of TGe-Mice. In contrast, MNC stimulated with Con A did not produce IFN activity when they were co-cultured with PAC of TI-Mice. These results suggest that the generation of suppressor macrophages in TI-Mice may be altered by the administration of Ge-132.
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002056:Burns / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology; D004768:Enterotoxins / Q000494:pharmacology; D005260:Female; D005857:Germanium / Q000633:toxicity*; D007369:Interferon Inducers / Q000494:pharmacology; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D007700:Kinetics; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009522:Newcastle disease virus / Q000276:immunology; D009942:Organometallic Compounds / Q000633:toxicity*; D011070:Poly I-C / Q000494:pharmacology; D011422:Propionates
D000970:Antineoplastic Agents; D004768:Enterotoxins; D007369:Interferon Inducers; D009942:Organometallic Compounds; D011422:Propionates; D005857:Germanium; D003208:Concanavalin A; C066750:propagermanium; C026066:enterotoxin A, Staphylococcal; D007371:Interferon-gamma; D011070:Poly I-C