Urinary excretion of thiol conjugates of aflatoxin B1 in rats and hamsters.
After intraperitoneal injection of [3H]aflatoxin B1 in rats and hamsters, 24-h urinary excretion of aflatoxin B1 metabolites was examined in these species. Total excretion was 10-15% of the injected dose with hamster excreting 50% more than the rat. In both species, 60% of the total excretion was due to glucuronide and sulfate conjugates of hydroxy metabolites. Striking differences were observed in the excretion of both aflatoxin B1-diol and various thiol conjugates. Aflatoxin B1-glutathione and aflatoxin B1-cysteinylglycine were major thiol conjugates in rats and hamsters, respectively. An unknown metabolite believed to be mercapturic acid (N-acetylcysteine conjugate) of aflatoxin B1 was excreted by both species, hamster excreting twice as much as the rat.
D000111:Acetylcysteine / Q000652:urine; D016604:Aflatoxin B1; D000348:Aflatoxins / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002273:Carcinogens; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D004151:Dipeptides / Q000652:urine; D005978:Glutathione / Q000652:urine; D008297:Male; D008647:Mesocricetus; D051381:Rats; D013438:Sulfhydryl Compounds / Q000652:urine*
D000348:Aflatoxins; D002273:Carcinogens; D004151:Dipeptides; D013438:Sulfhydryl Compounds; C028505:cysteinylglycine; D016604:Aflatoxin B1; D005978:Glutathione; D000111:Acetylcysteine