[Effect of fixation on immunolocalization of transferrin and albumin in the liver of the adult rat].
The influence of the fixation procedure on the localization of albumin and transferrin in adult rat liver has been carried out using an indirect immunoperoxidase technique at the light and electron microscopic levels. Perfusion and immersion fixations with different concentrations of paraformaldehyde (with or without addition of glutaraldehyde) have been investigated. According to the mode of fixation (perfusion versus immersion) and the concentration of the fixative, the number of albumin and transferrin containing hepatocytes could vary from 10% to 100%, and different labeling patterns could be observed at the electron microscopic level. For the same concentration of fixative, a perfusion fixation induces a less intense labeling than an immersion fixation. Thus similar results are obtained after immersion fixation in 6% paraformaldehyde + 0.25% glutaraldehyde or after perfusion fixation in 4% paraformaldehyde + 0.025% glutaraldehyde. Similar data are noticed after immersion fixation in 4% paraformaldehyde or after perfusion fixation in 1% paraformaldehyde + 0.025% glutaraldehyde. Moreover, perfusion fixation induced a more fine cell structure preservation than immersion fixations and avoided the appearance of zones of fixation.
D000418:Albumins / Q000032:analysis*; D000818:Animals; D005557:Formaldehyde; D005976:Glutaral; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D008099:Liver / Q000032:analysis*; D008297:Male; D008722:Methods; D008854:Microscopy, Electron; D010477:Perfusion; D011108:Polymers; D051381:Rats; D011919:Rats, Inbred Strains; D014168:Transferrin / Q000032:analysis*
D000418:Albumins; D007074:Immunoglobulin G; D011108:Polymers; D014168:Transferrin; D005557:Formaldehyde; D005976:Glutaral; C003043:paraform
Influence de la fixation sur l'immunolocalisation de la transferrine et de l'albumine dans le foie de rat adulte.