[Effect of gamma radiation on the immunobiological and immunochemical properties of cholera exotoxin. IV. The biological and immunochemical properties of purified irradiated choleragen].
The results of investigations carried out to study the effect of gamma radiation on the properties of the purified preparations of cholera exotoxin are presented. Irradiation has been shown to decrease the anterotoxicity of purified choleragen and the activity of its permeability factor, depending on the radiation dose. The investigations have revealed that in purified toxin enterotoxicity is completely inactivated with a lover radiation dose than in crude toxin filtrate (25 kGy). In immunochemical reactions the increase of the electrophoretic mobility of the choleragen components, correlated with the increase of the radiation dose, and the reduced number of protein zones have been observed. The irradiated preparations of purified choleragen have been found to retain their immunogenic properties and serological activity.
D000818:Animals; D002772:Cholera Toxin / Q000276:immunology / Q000302:isolation & purification / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004591:Electrophoresis, Polyacrylamide Gel; D005720:Gamma Rays; D007120:Immunochemistry; D005779:Immunodiffusion; D007421:Intestine, Small / Q000187:drug effects; D011233:Precipitin Tests; D011817:Rabbits; D013997:Time Factors; D014734:Vibrio cholerae
D002772:Cholera Toxin
Vliianie gamma-izlucheniia na immunobiologicheskie i immunokhimi-cheskie svoĭstva kholernogo ékzotoksina. Soobshchenie IV. Biologicheskie i immunokhimicheskie svoĭstva ochishchennogo obluchennogo kholerogena.