Ultrastructural analysis of human plasmacytoma cells prepared on affinity beads after exposure to anti-idiotype antibodies.
Mononuclear cells from a bone marrow infiltrated by plasmacytoma cells were examined by electron microscopy. An analysis was performed according to different cytoarchitectural forms of the endoplasmic reticulum (ER). Six types of plasma cells could be distinguished. The bone marrow cells were treated with an anti-idiotype antiserum from a guinea pig prepared against the patient's monoclonal serum protein and with a FITC conjugated anti-guinea pig antiserum from the rabbit as second layer. Then the cells were passed through an affinity gel column with anti-FITC antibodies. The original preparation and the cells separated on the affinity gel were analysed by electron microscopy. It was found that an ultrastructurally distinct type of plasma cell was enriched 3.5-fold over the original sample by the separation procedure.
D000888:Antibodies, Anti-Idiotypic; D002469:Cell Separation; D002846:Chromatography, Affinity; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008854:Microscopy, Electron; D010954:Plasmacytoma / Q000276:immunology / Q000473:pathology / Q000648:ultrastructure*; D013861:Thiocyanates
D000888:Antibodies, Anti-Idiotypic; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007130:Immunoglobulin Idiotypes; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate