Transport systems for lysine, thialysine and selenalysine in E. coli KL16.
Two lysine transport systems have been identified in E. coli KL16. They differ in their affinity for lysine, one showing a KM of 0.36 microM and the other a KM of 4.7 microM. Different compounds with chemical similarities to lysine were tested for their capacity to interfere with lysine transport. Among these only thialysine and selenalysine competitively inhibit lysine transport. The inhibition is on both transport systems. Thialysine shows a KI of 4 microM for the low affinity system and a KI of 8 microM for the high affinity system. Selenalysine shows values of 6 microM and 12 microM respectively.
D001692:Biological Transport; D003470:Culture Media; D003545:Cysteine / Q000031:analogs & derivatives* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism*; D016566:Organoselenium Compounds; D012643:Selenium / Q000378:metabolism*
D003470:Culture Media; D016566:Organoselenium Compounds; C000204:S-2-aminoethyl cysteine; C011351:selenalysine; D012643:Selenium; D008239:Lysine; D003545:Cysteine