In vivo binding of N-n-propylnorapomorphine in the rat striatum: quantification after lesions produced by kainate, 6-hydroxydopamine and decortication.
The neuronal localization of in vivo N-n-propylnorapomorphine (NPA) binding in the rat striatum was studied using 3 types of lesions. Striatal dopamine (DA) receptor densities (Bmax) were estimated from the relationships between total striatal and cerebellar NPA accumulation. A Bmax of 26.9 +/- 1.6 fmol X mg-1 wet weight tissue was found in the striata of non-lesioned (unoperated) rats. Similar values were obtained for striata with 6-hydroxydopamine-lesioned dopaminergic fibres. Kainate (KA)-lesioned striata contained 4.6 +/- 0.5 fmol X mg-1 saturable NPA binding sites. After unilateral decortication the receptor densities were in both striata resulting in ipsi- and contralateral Bmax values of 23 and 36 fmol X mg-1 respectively. With a tracer dose of [3H]NPA less radioactivity accumulated in the KA-lesioned striatum, while after unilateral destruction of the dopaminergic pathway more radioactivity was found in the ipsilateral striatum and no bilateral differences in striatal radioactivity concentration were found after unilateral cortical ablation. These observations show that all in vivo saturable striatal NPA binding sites are situated on striatal neurons and cortico-striatal afferents and that the effects of lesions on striatal DA receptor densities cannot be predicted from bilateral differences in the accumulation of tracer doses of [3H]NPA.
D000818:Animals; D001058:Apomorphine / Q000031:analogs & derivatives* / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D003655:Decerebrate State / Q000378:metabolism*; D006892:Hydroxydopamines / Q000494:pharmacology*; D007608:Kainic Acid / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D016627:Oxidopamine; D011759:Pyrrolidines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D006892:Hydroxydopamines; D011759:Pyrrolidines; C009074:N-n-propylnorapomorphine; D016627:Oxidopamine; D001058:Apomorphine; D007608:Kainic Acid