Development of a highly sensitive enzyme-immunoassay for serum carbonic anhydrase-III.
A highly sensitive sandwich enzyme-immunoassay (EIA) for human muscle carbonic anhydrase isozyme III (CA-III) has been developed using microplate as a solid-phase and peroxidase as a labelled enzyme. The assay can detect levels as low as 2 ng/ml when 20 microliter of sample sera were used. Sera from patients with various neurological diseases were studied using this method, and elevated serum CA-III levels were found in patients with Duchenne muscular dystrophy, limb-girdle dystrophy, fascioscapulohumeral dystrophy, polymyositis and amyotrophic lateral sclerosis. The values correlated well with the results of radioimmunoassay (RIA), with a correlation coefficient of 0.92 (P less than 0.001). We feel EIA is preferable to RIA for its simple methodology.
D002256:Carbonic Anhydrases / Q000097:blood*; D003937:Diagnosis, Differential; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000097:blood*; D009468:Neuromuscular Diseases / Q000201:enzymology*; D011863:Radioimmunoassay; D012016:Reference Values
D007527:Isoenzymes; D002256:Carbonic Anhydrases