Glucuronidation in the reindeer: dietary modification in the UDP-glucuronosyltransferase activity with 4-nitrophenol, 1-naphthol and phenolphthalein as acceptors.
The UDP-glucuronosyltransferase activity towards 4-nitrophenol, 1-naphthol and phenolphthalein was measured from the hepatic microsomes of the reindeer (Rangifer tarandus tarandus) after summer, autumn and winter feeding periods. The microsomes were digested with trypsin or digitonin. The UDP-glucuronosyltransferase activity with 4-nitrophenol and 1-naphthol as aglycones was lower in reindeer on winter food than in ones on summer food after trypsin and digitonin digestion. The activity towards phenolphthalein was the same in each feeding period. The different seasonal feeding affects the structure of microsomal membranes and this is reflected as modifications of the UDP-glucuronosyltransferase towards different substrates.
D000818:Animals; D004032:Diet; D014453:Glucuronosyltransferase / Q000378:metabolism*; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000201:enzymology; D009284:Naphthols; D009596:Nitrophenols; D020113:Phenolphthalein; D010635:Phenolphthaleins; D012053:Reindeer / Q000378:metabolism*; D012621:Seasons; D013379:Substrate Specificity
D009284:Naphthols; D009596:Nitrophenols; D010635:Phenolphthaleins; C029350:1-naphthol; D020113:Phenolphthalein; D014453:Glucuronosyltransferase; C024836:4-nitrophenol