A face mask for estimation of whole body leucine turnover in unanesthetized beagle dogs.
As part of a long-term study of protein and energy metabolism in Beagle dogs, a latex face mask was developed to facilitate respiratory gas collection. The mask described here is easily assembled using readily available materials and meets the following criteria: small dead air space, lightweight, readily acceptable by conscious dogs over a prolonged period of time, leakproof , and inexpensive. The mask has been successfully used with L-[1-13C] leucine to measure 13CO2 excretion rates in studies of leucine metabolism.
D000818:Animals; D002245:Carbon Dioxide / Q000032:analysis; D002250:Carbon Radioisotopes; D004285:Dogs / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D007840:Latex; D007930:Leucine / Q000097:blood / Q000378:metabolism*; D008297:Male; D008397:Masks
D002250:Carbon Radioisotopes; D007840:Latex; D002245:Carbon Dioxide; D007930:Leucine