title: The histochemical characterization of the coupling state of skeletal muscle mitochondria.
abstract: Isolated mitochondria from skeletal muscles of human and animals with neuromuscular diseases may reveal a loosely coupled state of oxidative phosphorylation, which is characterized by a normal phosphorylation in the presence of a phosphate acceptor and a maximal respiration in the absence of a phosphate acceptor. Moreover in these cases activity of mitochondrial Mg2+-stimulated ATPase is strongly increased and cannot be stimulated by the uncoupler 2,4-dinitrophenol. In this communication a histochemical technique for the demonstration of activity of mitochondrial Mg2+-stimulated ATPase to characterize the coupling state of muscle mitochondria in tissue sections, is described. This tissue-saving technique is especially suitable for the study of human skeletal muscle diseases.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D004140:Dinitrophenols / Q000494:pharmacology; D004285:Dogs; D006651:Histocytochemistry; D006801:Humans; D008274:Magnesium / Q000494:pharmacology; D051379:Mice; D008931:Mitochondria, Muscle / Q000378:metabolism*; D009468:Neuromuscular Diseases / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D011817:Rabbits; D051381:Rats
chemical_list: D004140:Dinitrophenols; D000251:Adenosine Triphosphatases; D008274:Magnesium