title: Variant endothelial cells from human carotid artery in culture.
abstract: Endothelial cells were cultured from the carotid artery with thickened intima comprised of two to five layers of smooth muscle cells, isolated from a 19-yr-old female, who died from an accident. The cells were grown and subcultured in Medium 199 supplemented with 20% heat inactivated fetal bovine serum. The cells are still viable at present, the 22nd passage. The cultured cells were found to have the following characteristics: existence of Factor VIII-related surface antigen and prostacyclin synthesis slightly less than that for typical endothelial cells. The most outstanding feature was the formation by an individual cell of a single ring, and a composite ring formed by two to five cells. Neither the synthesis of an angiotensin converting enzyme nor that of a Weibel-Palade body could be detected by electron microscopy. The cultured cells possessed only a few characteristics specific for typical endothelial cells and were designated as variant endothelial cells.
mesh_terms: D000328:Adult; D000941:Antigens / Q000032:analysis; D001485:Basement Membrane / Q000648:ultrastructure; D002339:Carotid Arteries / Q000502:physiology* / Q000648:ultrastructure; D002462:Cell Membrane / Q000648:ultrastructure; D002478:Cells, Cultured; D004727:Endothelium / Q000502:physiology / Q000648:ultrastructure; D011464:Epoprostenol / Q000096:biosynthesis; D005169:Factor VIII / Q000032:analysis / Q000276:immunology; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008854:Microscopy, Electron; D014841:von Willebrand Factor
chemical_list: D000941:Antigens; D014841:von Willebrand Factor; D005169:Factor VIII; D011464:Epoprostenol