title: Hybrid protein containing heavy subunit of haptoglobin and light subunit of immunoglobulin G.
abstract: Dimeric hybrid protein molecule consisting of disulfide-linked beta (heavy) subunit of haptoglobin (Hp) and L (light) subunit of immunoglobulin G, was synthesized. Molecular mass of the hybrid was 62.0 kd, sedimentation constant B20 = 2.43. Hemoglobin binding activity of the beta X L hybrid as compared with that of native Hp accounted for 25%. In immunological cross-reactivity the hybrid was found to be deficient in some antigenic determinants present in Hp and in Hp beta chain. Hybridization carried out in three-component mixtures (Hp beta subunits, Hp alpha (light) subunits, IgG-L) showed, that besides reconstituted tetramers of Hp (2 alpha X 2 beta) always the hybrid beta X L was formed, even at an excess of Hp alpha, an excess of IgG-L prevented the formation of tetrameric molecules. Conformational similarity of Hp alpha and IgG-L as disulfide linked with Hp beta is reflected in the interaction of respective conjugates with hemoglobin.
mesh_terms: D004220:Disulfides; D004589:Electrophoresis, Disc; D006242:Haptoglobins / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000378:metabolism*; D007147:Immunoglobulin Light Chains / Q000378:metabolism*; D008970:Molecular Weight; D011485:Protein Binding; D055503:Protein Multimerization
chemical_list: D004220:Disulfides; D006242:Haptoglobins; D006454:Hemoglobins; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains