Production of a monospecific antiserum to cathepsin L: the histochemical location of enzyme in rabbit fibroblasts.
A polyclonal antibody against rabbit cathepsin L was raised in goats and shown to be specific for both active and inactive enzyme. Using this antibody we have examined the distribution of cathepsin L in primary rabbit skin fibroblasts by immunohistochemistry and found that all the enzyme is located within lysosomal granules. At confluence many cathepsin-L-containing lysosomes were seen in each cell. A new but nonspecific histochemical substrate for cathepsin L was tested and a similar distribution was obtained. Our results indicate that the immunohistochemical technique can be reliably employed for the specific location of cathepsin L in cells and tissues.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D056668:Cathepsin L; D002403:Cathepsins / Q000032:analysis* / Q000276:immunology; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D005347:Fibroblasts / Q000201:enzymology; D006041:Goats / Q000276:immunology; D006651:Histocytochemistry; D005779:Immunodiffusion; D011817:Rabbits; D012867:Skin / Q000201:enzymology*; D014018:Tissue Distribution
D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D056668:Cathepsin L