title: Characterization of the precursor form of the exocellular levansucrase from Bacillus subtilis.
abstract: Expression of the cloned levansucrase gene (sacB) was demonstrated in E. coli minicells by assay of the enzyme in crude extracts, SDS-polyacrylamide gel electrophoresis and immunoblotting. The existence of a precursor form of the enzyme of MW 53000 was also demonstrated and confirmed by the DNA sequence corresponding to the NH2 terminal region of the protein.
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004792:Enzyme Precursors / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology; D005796:Genes; D005798:Genes, Bacterial; D006602:Hexosyltransferases / Q000235:genetics*; D010957:Plasmids
chemical_list: D004269:DNA, Bacterial; D004792:Enzyme Precursors; D006602:Hexosyltransferases; C021513:levansucrase