Passage of molecules across the intercellular bridge between post-mitotic daughter cells.
Living mitotic HeLa cells were microinjected with sodium fluorescein, fluorescein isothiocyanate (FITC)-labelled IgG, and Lucifer Yellow-CH to determine if the intracellular bridge and midbody forms a barrier to the migration of these molecules. All three fluorescent molecules were found to pass across the intercellular bridge. The post-mitotic cells maintained a molecular patency across the intercellular bridge that persisted for considerable lengths of time, extending into the next cell cycle.
D002450:Cell Communication; D019793:Fluorescein; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007074:Immunoglobulin G; D007365:Intercellular Junctions / Q000378:metabolism*; D007546:Isoquinolines / Q000378:metabolism; D008845:Microinjections; D008938:Mitosis; D013861:Thiocyanates / Q000378:metabolism
D005452:Fluoresceins; D007074:Immunoglobulin G; D007546:Isoquinolines; D013861:Thiocyanates; C017475:lucifer yellow; D016650:Fluorescein-5-isothiocyanate; D019793:Fluorescein