[Isolation of strains of the virus of hemorrhagic fever with renal syndrome in cell culture].
Four strains of hemorrhagic fever with renal syndrome (HFRS) virus were isolated from the lungs of Cl. glareolus caught in natural foci of HFRS in the European USSR. Preliminary experiments using enzyme-immunoassay (EIA) established the appurtenance of HFRS antigen in lung suspensions to the "western" serotype. Four strains were isolated in Vero-E6 cell culture after several blind passages and identified as the "western" serotype of HFRS virus. One stram originally isolated in Wistar rats and passaged in them 4 times and then adapted to Vero-E6 cells showed a closer antigenic relationship with the "eastern" HFRS virus serotype.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D003411:Arvicolinae / Q000382:microbiology; D001486:Bashkiria; D002460:Cell Line; D002522:Chlorocebus aethiops; D005455:Fluorescent Antibody Technique; D006476:Orthohantavirus / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007163:Immunosorbent Techniques; D007668:Kidney; D008168:Lung / Q000382:microbiology; D012328:RNA Viruses / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D014776:Virus Cultivation
D000914:Antibodies, Viral
Vydelenie shtammov virusa gemorragicheskoĭ likhoradki s pochechnym sindromom v kul'ture kletok.