An enzyme immunoassay for urinary C-reactive protein.
Because urinary C-reactive protein (CRP) has potential as a diagnostic marker, a sensitive enzyme immunoassay has been developed to measure this substance. This assay involves immobilizing antibody to CRP on a plastic surface, followed sequentially by addition of sample to be assayed, anti-CRP labeled with biotin, avidin conjugated with enzyme, and substrate. As little as 0.5 ng of CRP can be detected by the assay, which is reproducible and correlates well with a radial immunodiffusion method. Because the assay is inhibited by urine samples having a low pH or high urea concentration, it is necessary to pretreat the samples by solvent exchange in a commercial, disposable concentration unit. Application of the assay to clinical specimens gives results similar to those obtained previously by a complement fixation assay.
D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D001710:Biotin; D002097:C-Reactive Protein / Q000276:immunology / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D012016:Reference Values; D013696:Temperature; D013997:Time Factors; D001749:Urinary Bladder Neoplasms / Q000652:urine
D001710:Biotin; D002097:C-Reactive Protein