Assays of glucose tolerance factor and its mode of action, studied with brewer's yeast.
Glucose tolerance factor (GTF) has usually been assayed by manometric measurement of CO2 evolved when glucose was metabolizing glucose. By using 14C labeled substrates it has been shown that GTF increases the decarboxylation of pyruvate to ethanol and CO2. Thus in addition to measuring CO2 evolution, the enzymatic estimation of the increased ethanol production can be used to assay GTF. A further effect of GTF was to cause increased carboxylation of pyruvate to substrates that are used in the biosynthesis of cell substance. The metabolic sites of action of GTF are discussed.
D000332:Aerobiosis; D000596:Amino Acids / Q000378:metabolism*; D000693:Anaerobiosis; D001693:Biological Transport, Active; D002245:Carbon Dioxide / Q000378:metabolism; D002857:Chromium / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008928:Mitochondria / Q000378:metabolism; D009539:Nicotinic Acids / Q000378:metabolism*; D011766:Pyruvate Carboxylase / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D000596:Amino Acids; D009539:Nicotinic Acids; D011773:Pyruvates; C015240:glucose tolerance factor; D002857:Chromium; D002245:Carbon Dioxide; D000431:Ethanol; D019289:Pyruvic Acid; D003847:Deoxyglucose; D011766:Pyruvate Carboxylase