Selective affinity of protein A containing staphylococci for monomeric and polymeric IgG.
Protein A containing staphylococci were saturated with human monomeric IgG (mIgG) and cross-linked with glutaraldehyde. The resulting material (SMG) preferentially bound aggregated IgG (aIgG) and soluble immune complexes (CIC). One milliliter of a 10% suspension of SMG bound approximately 30 micrograms of mIgG and 1000 micrograms of aIgG and CIC. The binding of aIgG to SMG was reduced to approximately 50% at a 20-fold excess of mIgG over aIgG. CIC and aIgG could be released from SMG by elution with 3 M KSCN. The results indicate that SMG can be used for identification and removal of CIC in patient plasma.
D000936:Antigen-Antibody Complex / Q000032:analysis / Q000378:metabolism; D001666:Binding Sites, Antibody / Q000187:drug effects; D002949:Citraconic Anhydrides; D003432:Cross-Linking Reagents; D005976:Glutaral; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D007158:Immunologic Techniques; D007164:Immunosorbents / Q000378:metabolism; D046911:Macromolecular Substances; D013205:Staphylococcal Protein A / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000378:metabolism
D000936:Antigen-Antibody Complex; D002949:Citraconic Anhydrides; D003432:Cross-Linking Reagents; D007074:Immunoglobulin G; D007164:Immunosorbents; D046911:Macromolecular Substances; D013205:Staphylococcal Protein A; D005976:Glutaral