title: The nucleotide sequences of the 5 S rRNAs of seven molds and a yeast and their use in studying ascomycete phylogeny.
abstract: The sequences of the 5 S rRNAs isolated from 8 ascomycete species belonging to the genera Aspergillus, Penicillium, Acremonium and Candida are reported. Two of the examined strains each yielded a mixture of 3 slightly different 5 S RNAs, which were individually sequenced after fractionation. A previously published sequence for Aspergillus nidulans 5 S RNA was found to contain errors. Reconstruction of an evolutionary tree based on 5 S RNA sequences showed that the 16 presently examined ascomycetes form three clusters. The same threefold partition can be observed in the secondary structure pattern, each cluster showing a slightly different variant of the general 5-helix model for 5 S rRNA (De Wachter, Chen and Vandenberghe (1982) Biochimie 64, 311-329), and different sets of secondary structure equilibrium forms in helices C and E of the aforementioned model.
mesh_terms: D001231:Aspergillus flavus / Q000235:genetics; D001233:Aspergillus nidulans / Q000235:genetics; D001234:Aspergillus niger / Q000235:genetics; D001483:Base Sequence; D002176:Candida albicans / Q000235:genetics; D005658:Fungi / Q000235:genetics*; D003904:Mitosporic Fungi / Q000235:genetics; D010407:Penicillium / Q000235:genetics; D010408:Penicillium chrysogenum / Q000235:genetics; D010802:Phylogeny; D012335:RNA, Ribosomal; D013045:Species Specificity
chemical_list: D012335:RNA, Ribosomal