Effect of silver on whole cells and spheroplasts of a silver resistant Pseudomonas aeruginosa.
Electron micrographs of a silver resistant strain of Pseudomonas aeruginosa grown in the presence of 50 micrograms/ml Ag+ indicate that the surviving cells are resistant at the level of the cytoplasmic membrane. Spheroplasts of the resistant strain were not lysed by 1 h contact with 8 micrograms/ml Ag+, but were 36% and 22% lysed by 1 h contact with 20 micrograms/ml chlorhexidine and 40 micrograms/ml polysorbate 80, respectively. Spheroplasts of a silver sensitive strain of P. aeruginosa were lysed by 22% on contact with 1 microgram/ml Ag+ for 1 h.
D002462:Cell Membrane / Q000187:drug effects; D002710:Chlorhexidine / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D007700:Kinetics; D011136:Polysorbates / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology; D012834:Silver / Q000494:pharmacology*; D013104:Spheroplasts / Q000187:drug effects* / Q000502:physiology
D011136:Polysorbates; D012834:Silver; D002710:Chlorhexidine