[Production of monoclonal autoantibodies inhibiting the secretion of insulin by the islets of Langerhans].
Monoclonal autoantibodies cytotoxic for murine islet cells have been obtained by cell fusion using spleen cells from non-immunized diabetic C57BL/KsJ (db/db) Mice. These monoclonals, of IgG2a subclass, blocked in vitro insulin secretion induced by arginine in Mouse pancreatic cells.
D000818:Animals; D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D003602:Cytotoxicity, Immunologic; D003921:Diabetes Mellitus, Experimental / Q000276:immunology / Q000503:physiopathology; D005260:Female; D007328:Insulin / Q000378:metabolism*; D007330:Insulin Antibodies; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D007328:Insulin; D007330:Insulin Antibodies
Production d'autoanticorps monoclonaux inhibant la libération d'insuline par les îlots de Langerhans.