Characterization of anti-N-acetyl-D-glucosamine antibodies elicited through haptenated liposomes.
Antiserum was raised in rabbits against p-aminophenyl 2-acetamido-2-deoxy-beta-D-glucoside covalently coupled to phosphatidylaminoethanol-containing lecithin liposomes. The affinity-purified antibodies were found to be IgM. The properties of the antibodies were studied by ligand inhibition of quantitative precipitation, fluorescence titration with 2-acetamido-2-deoxy-D-glucose, and complement lysis of haptenated liposomes.
D000117:Acetylglucosamine / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D005944:Glucosamine / Q000031:analogs & derivatives*; D005960:Glucosides / Q000276:immunology; D006168:Guinea Pigs; D006241:Haptens / Q000276:immunology*; D005779:Immunodiffusion; D007075:Immunoglobulin M / Q000302:isolation & purification; D008081:Liposomes / Q000276:immunology*; D011817:Rabbits
D000906:Antibodies; D005960:Glucosides; D006241:Haptens; D007075:Immunoglobulin M; D008081:Liposomes; C042263:4-aminophenyl-2-acetamido-2-deoxyglucoside; D005944:Glucosamine; D000117:Acetylglucosamine