Transcriptional control of human diploid fibroblast collagen synthesis by gamma-interferon.
Recombinant gamma-interferon (rec gamma-IFN) caused potent inhibition of collagen synthesis by cultured confluent human diploid fibroblasts in a dose-dependent manner. Gel electrophoresis of the newly synthesized proteins from the culture media of rec gamma-IFN-treated fibroblasts demonstrated a selective depression of procollagen without a significant change in non-collagenous proteins. Dot blot hybridization to a Type I procollagen cDNA probe showed that the inhibition of collagen production was accompanied by a decrease in the levels of collagen mRNA. These results indicate that rec gamma-IFN is capable of exerting transcriptional modulation of collagen biosynthesis and suggest that it may play an important role in regulation of normal and pathologic fibrogenesis.
D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis / Q000235:genetics*; D004247:DNA / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006909:Hydroxyproline / Q000096:biosynthesis; D007371:Interferon-gamma / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D011392:Proline / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D012867:Skin / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects*
D012333:RNA, Messenger; D007371:Interferon-gamma; D003094:Collagen; D004247:DNA; D011392:Proline; D006909:Hydroxyproline