Synthesis and expression of RNase T1 gene.
In order to obtain knowledges about structure-function relationship of ribonuclease T1, we synthesized a structural gene for RNase T1 and several its modified genes. Using amino acid codons frequently used in Escherichia coli we designed genes consist of 328 X 2 bases. Synthesis of oligodeoxynucleotides with 9-20 base lengths was performed by 1% polystyrene supported triester approach and resulting 42 oligomers were joined together using T4 DNA ligase. The product was analyzed and utilyzed to construct expression vectors, which produced effectively fused proteins.
D000595:Amino Acid Sequence; D001483:Base Sequence; D004722:Endoribonucleases / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D005813:Genes, Synthetic; D010957:Plasmids; D006163:Ribonuclease T1 / Q000235:genetics*
D004722:Endoribonucleases; D006163:Ribonuclease T1