Perfusion preservation of cadaver rat pancreas: II. Culture after perfusion and successful transplantation.
We designed a new process for culturing pancreatic islets and applied this method to cultures of rat pancreatic islets which had degenerated during preservation by the perfusion-method for 6 hours under the condition of hypothermia and oxygenation. The objective was to determine the extent of the original function. Transplantation of these so-treated islets was also attempted. When pancreatic islets isolated from the pancreas after 6-hour-perfusion were cultured, morphological restoration was apparent within the first 3-4 days. Insulin contents of the culture media renewed every 3 days, ranged from 851 to 1,134 microU/ml/two islets during culture period of 21 days. In the glucose-loading test, insulin secretion of the islets was the same as that of islets in the control experiments. Transplantation of these islets into the portal vein of streptozotocin-induced diabetic rats resulted in a good recovery from the diabetic state.
D000818:Animals; D002478:Cells, Cultured; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism; D016381:Islets of Langerhans Transplantation; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D010179:Pancreas; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin