Mobilization of arachidonic acid between diacyl and ether phospholipids in rabbit alveolar macrophages.
The metabolism of 20:4 (arachidonic acid) in alkenylacyl, alkylacyl and diacyl lipid classes in choline glycerophospholipids (CGP) and ethanolamine glycerophospholipids (EGP) in rabbit alveolar macrophages was examined. [3H]20:4 was very rapidly incorporated into diacyl glycerophosphocholine (GPC). After the removal of free 20:4, the radioactivity was gradually lost from diacyl GPC. Concomitantly, the radioactivities in alkylacyl GPC and alkenylacyl glycerophosphoethanolamine (GPE) were increased, indicating that 20:4 was mobilized from diacyl GPC to alkylacyl GPC and alkenylacyl GPE. The mobilization was considered to be a 20:4-specific event. The gradual accumulation of 20:4 in ether phospholipids leads to a high abundance of 20:4 in these lipids. These results suggest metabolic relationships between 20:4 and ether phospholipids, including platelet-activating factor (PAF).
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D004987:Ethers / Q000378:metabolism; D005997:Glycerylphosphorylcholine / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000166:cytology*; D011817:Rabbits; D013329:Structure-Activity Relationship
D001095:Arachidonic Acids; D004987:Ethers; D010714:Phosphatidylethanolamines; D016718:Arachidonic Acid; D005997:Glycerylphosphorylcholine