Determination of beta-galactosidase activity in the intestinal tract of mice by ion-exchange high-performance liquid chromatography using epsilon-N-1-(1-deoxylactulosyl)-L-lysine as substrate.
epsilon-N-1-(1-Deoxylactulosyl)-L-lysine was synthesized and used as a substrate to assay beta-galactosidase activity. epsilon-N-1-(1-Deoxylactulosyl)-L-lysine and its degradation product epsilon-N-1-(1-deoxyfructosyl)-L-lysine were detected by high-voltage paper electrophoresis and ion-exchange high-performance liquid chromatography. The beta-galactosidase activity in different parts of the intestinal tract of germ-free and control mice was determined and compared with a beta-galactosidase activity which degrades lactose at pH 8.5 and 5.0 and which corresponded with bacterial and host enzymatic activities, respectively.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004187:Disaccharides; D005696:Galactosidases / Q000032:analysis*; D005856:Germ-Free Life; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000201:enzymology*; D007792:Lactulose / Q000031:analogs & derivatives*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D001616:beta-Galactosidase / Q000032:analysis*
D000596:Amino Acids; D004187:Disaccharides; C040454:epsilon-N-1-(1-deoxylactulosyl)lysine; D007792:Lactulose; D005696:Galactosidases; D001616:beta-Galactosidase