The carbon dioxide hydration activity of the sulfonamide-resistant carbonic anhydrase from the liver of male rat: pH independence of the steady-state kinetics.
The steady-state kinetic constants for the catalysis of CO2 hydration by the sulfonamide-resistant and testosterone-induced carbonic anhydrase from the liver of the male rat has been determined by stopped-flow spectrophotometry. The turnover number was 2.6 +/- 0.6 X 10(3) s-1 at 25 degrees C, and was invariant with pH ranging from 6.2 to 8.2 within experimental error. The Km at 25 degrees C was 5 +/- 1 mM, and was also pH independent. These data are in quantitative agreement with earlier findings of pH-independent CO2 hydration activity for the mammalian skeletal muscle carbonic anhydrase isozyme III. The turnover numbers for higher-activity isozymes I and II are strongly pH dependent in this pH range. Thus, the kinetic status of the male rat liver enzyme is that of carbonic anhydrase III. This finding is consistent with preliminary structural and immunologic data from other laboratories.
D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism*; D002257:Carbonic Anhydrase Inhibitors; D002256:Carbonic Anhydrases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D013053:Spectrophotometry; D013449:Sulfonamides / Q000494:pharmacology*; D013739:Testosterone / Q000494:pharmacology; D013830:Thiadiazoles / Q000494:pharmacology
D002257:Carbonic Anhydrase Inhibitors; D007527:Isoenzymes; D013449:Sulfonamides; D013830:Thiadiazoles; D002245:Carbon Dioxide; D013739:Testosterone; C000471:chlorzolamide; D002256:Carbonic Anhydrases