Isolation and characterization of pock-forming plasmids for Streptomyces griseus from soil actinomycetes.
Thirty independent actinomycetes strains carrying plasmids were isolated from soil. These plasmids were purified as cccDNA by CsCl-EtBr equilibrium density-gradient centrifugation. Plasmids that induce "pocks", namely formation of circular zones of sporulation-inhibition, were selected by protoplast transformation of streptomycin-producing strain, Streptomyces griseus ATCC10137. Six pock-forming plasmids, pOA7, pOA11, pOA15, pOA23, pOA29 and pOA30, were obtained, and their cleavage maps, transformation frequencies, and copy numbers, as well as their stability, are described.
D000192:Actinomycetales / Q000235:genetics*; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000096:biosynthesis; D010957:Plasmids; D012988:Soil Microbiology; D013305:Streptomyces griseus / Q000235:genetics*; D013307:Streptomycin / Q000096:biosynthesis; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D013307:Streptomycin