A rat monoclonal antibody that binds specifically to mouse T lymphoblasts and inhibits IL 2 receptor functions: a putative anti-IL 2 receptor antibody.
To obtain an anti-interleukin 2 (IL 2) receptor antibody, we immunized rats with phorbol myristate acetate-pulsed mouse T lymphoblasts. The spleen cells of the rats were fused with rat myeloma cells. Several stable clones of hybridoma cells were obtained that produced monoclonal antibodies (mAb) reacting specifically with mouse T lymphoblasts. One of these mAb, AMT-13, showed characteristics of a putative anti-IL 2 receptor antibody. As tested by FACS analysis, the mAb AMT-13 binds to murine T lymphoblasts or to IL 2-dependent T cell lines, but not to B lymphoblasts, thymocytes, splenocytes, lymph node cells, or bone marrow cells. The mAb AMT-13 inhibited in a species-specific and dose-dependent manner: i) the capacity of mouse T lymphoblasts to absorb IL 2 activity, and ii) the capacity of mouse T lymphoblasts to proliferate in response to IL 2. The function of T lymphoblasts of rat origin was not affected by the mAb AMT-13. The time course of the acquisition by mitogen-stimulated spleen cells of the capacity to absorb IL 2 activity was paralleled by that of their capacity to bind the mAb AMT-13. Preliminary biochemical analysis of the antigen recognized by AMT-13 revealed a major component with an apparent m.w. of 50,000 to 60,000. According to these data, the mAb AMT-13 seems to be directed against an antigenic determinant of the murine IL 2 receptor molecule.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000502:physiology*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D003208:Concanavalin A / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D007376:Interleukin-2 / Q000378:metabolism / Q000502:physiology; D007700:Kinetics; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D051381:Rats; D011917:Rats, Inbred Lew; D011948:Receptors, Antigen, T-Cell / Q000276:immunology* / Q000302:isolation & purification; D011971:Receptors, Immunologic / Q000276:immunology* / Q000302:isolation & purification; D015375:Receptors, Interleukin-2; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D003208:Concanavalin A