Cytotoxicity of chemical carcinogens towards human bronchial epithelial cells evaluated in a clonal assay.
Survival of human bronchial epithelial cells after administration of four chemical carcinogens was measured in a clonal assay. Human bronchial epithelial cells were obtained from outgrowths of explanted tissue pieces. Serum-free medium was used for both explant culture and clonal growth. The clonal assay could be performed on three substrata: plastic dishes alone, protein-coated dishes, and inactivated Swiss 3T3 cells. Several other cell lines supported clonal growth of the human cells. Fetal bovine serum was inhibitory to colony formation on plastic and protein-coated dishes, but had no effect on the growth of bronchial cells on 3T3 feeder cells. Little variation among individuals in cytotoxicity of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and K2CrO4, a possible human lung carcinogen, was observed, but in the case of benzo[a]pyrene (BP) and 7,12-dimethyl-benz[a]anthracene (DMBA), large variations in survival were found between cultures derived from different individuals.
D015127:9,10-Dimethyl-1,2-benzanthracene; D000328:Adult; D000368:Aged; D001564:Benzo(a)pyrene; D001980:Bronchi / Q000187:drug effects* / Q000473:pathology; D002273:Carcinogens; D002478:Cells, Cultured; D002840:Chromates / Q000633:toxicity; D004848:Epithelium / Q000187:drug effects; D005260:Female; D006801:Humans; D008297:Male; D008769:Methylnitronitrosoguanidine; D008875:Middle Aged; D017680:Potassium Compounds
D002273:Carcinogens; D002840:Chromates; D017680:Potassium Compounds; D008769:Methylnitronitrosoguanidine; D001564:Benzo(a)pyrene; D015127:9,10-Dimethyl-1,2-benzanthracene; C027373:potassium chromate(VI)