[Nucleic acid and protein synthesis of splenic lymphocytes of the rat under the influence of mucopolysaccharide-polysulfuric acid-esters (author's transl)].
The influence of three mucopolysaccharide-polysulfuric acid-esters (MPS) of different molecular weight on DNAase II-activity as well as on nucleic acid and protein synthesis of lymphocytes of rat spleen has been investigated in vitro. The results are summarized as follows: 1. DNAase II-activity (bovine spleen) was inhibited by MPS in a competitive manner. 2. At concentrations of greater than or equal to 10 microgram/ml MPS decreased the incorporation of 3H-thymidine and 14C-uridine into the nucleic acids of the cell suspension. 3. Incorporation of 3H-amino-acids into the lymphocytes protein was enhanced when MPS were added at concentrations of > 1 and > 10 microgram/ml, resp. At lower concentrations of the polyanions, a slight inhibition of protein synthesis was shown. 4. In the presence of MPS, incorporation of the radioactively labelled presursors into the acid-soluble fraction of the cells was enhanced. 5. The MPS-effects described increased with the molecular weight of the polyanions. It is suggested that polyanions like MPS may interfere rather non-specifically with cell membranes and with nucleic acid-polymerases.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D003851:Deoxyribonucleases / Q000037:antagonists & inhibitors; D006025:Glycosaminoglycans / Q000494:pharmacology*; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000378:metabolism*; D009696:Nucleic Acids / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D051381:Rats; D013154:Spleen / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D000596:Amino Acids; D006025:Glycosaminoglycans; D009696:Nucleic Acids; D003851:Deoxyribonucleases; D013936:Thymidine; D014529:Uridine
Nucleinsäure- und Proteinsynthese in Milzlymphozyten der Ratte unter dem Einfluss von Mucopolysaccharidpolyschwefelsäureestern.