Purification of human lysozyme from milk and pancreatic juice.
Human milk lysozyme was purified by heparin-Sepharose affinity chromatography and Sepharose 4B gel-permeation chromatography. This procedure was also found applicable to the purification of human pancreatic juice lysozyme. Double-diffusion analyses indicated that human milk lysozyme was immunochemically identical to human saliva and human pancreatic juice lysozyme. Based on the identity of the N-terminal 10-amino-acid-residue sequence analyzed, it was suggested that human milk lysozyme and human pancreatic juice lysozyme are identical molecular entities.
D000595:Amino Acid Sequence; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D005779:Immunodiffusion; D008875:Middle Aged; D008895:Milk, Human / Q000201:enzymology*; D009113:Muramidase / Q000302:isolation & purification*; D010189:Pancreatic Juice / Q000201:enzymology*; D012463:Saliva / Q000201:enzymology
D009113:Muramidase