[Characteristics of the growth of cells on a surface of limited area].
An original technique was used to study the growth pattern of cells of varying origin on limited areas (from 1 to 0.01 mm2). During cultivation on limited areas, the growth of primary and continuous fibroblast-like cells was inhibited, whereas that of normal and transformed epithelioid cells was not. The growth of fibroblast-like skin cells of diploid lines and human embryonal muscles was completely inhibited in cultivation on 0.1-0.02 mm2 areas and partially inhibited in cultivation on 0.03-0.16 mm2 areas. Other normal fibroblast-like cells used in the experiment were also marked by growth inhibition of varying degree during cultivation on limited areas. The technique suggested and the data thus obtained may be used for studying substrate-cell and cell-cell interactions, selection of epithelioid cells, and the development of optimal microcarrier technology for cell cultures.
D000818:Animals; D002143:Callitrichinae; D002460:Cell Line; D002478:Cells, Cultured / Q000502:physiology*; D002642:Chick Embryo; D006801:Humans; D012756:Sheep
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