The influence of anticoagulation on acid-base status and blood-gas analysis.
The results of acid-base status and blood-gas analysis can be influenced massively by incorrect sampling. We found that the addition of heparin has no great effect on blood-gas measurement. Citric acid and fluoride can also be admixed to blood samples in the usual concentrations without severe side-effects. The major error in the determination of blood gases and acid-base status is a consequence of even minimal dilution: filling the deadspace of a syringe with any diluent leads to unacceptable results. Hence, we conclude that blood specimens for determination of blood gases and acid-base status should be taken in specially prepared syringes containing the anticoagulant in the form of a dry substance. We think that citric acid is also acceptable for anticoagulation. Fluoride should be added in order to minimize alterations of pH due to anaerobic glycolysis.
D000136:Acid-Base Equilibrium / Q000187:drug effects*; D000925:Anticoagulants / Q000494:pharmacology*; D001800:Blood Specimen Collection / Q000592:standards; D002245:Carbon Dioxide / Q000097:blood; D006438:Hemodilution; D006801:Humans; D010100:Oxygen / Q000097:blood*
D000925:Anticoagulants; D002245:Carbon Dioxide; D010100:Oxygen