Sporulation promoting factor in vegetative cells of Bacillus subtilis.
A simple experimental system for detection of sporulation promoting factors was presented. This system showed that there was a sporulation promoting factor in the vegetative cells of Bacillus subtilis cultivated on nutrient agar for 9 hr (at stage T0). The factor was partially purified from the sonicate of vegetative cells by ethanol fractionation, gel filtration, chromatography and preparative gel electrophoresis, and it was identified as manganese-containing protein.
D001412:Bacillus subtilis / Q000032:analysis / Q000502:physiology*; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000502:physiology; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D008345:Manganese; D008858:Microscopy, Phase-Contrast; D013171:Spores, Bacterial / Q000032:analysis
D001426:Bacterial Proteins; D008345:Manganese