title: The detection of subclasses of granulosa cells with differing responsiveness to EGF, FGF, and gonadotropin preparations using an anchorage-independent clonal agar assay.
abstract: Subpopulations of granulosa cells of differing responsiveness to epidermal growth factor (EGF), fibroblast growth factor (FGF), pituitary gonadotropin preparations, and rat erythrocyte suspensions (RBC) have been detected using an anchorage-independent clonal agar assay. All growth factor preparations were capable of enhancing colony formation when added alone, and elicited cloning efficiencies as high as 35% when added to the culture system at optimal concentrations in a variety of combinations. The FGF preparation was the single most effective stimulator of colony formation, augmenting both colony numbers and colony size at concentrations as low as 50 ng/ml. However, unlike the other growth factors in this assay system, a plateau in responsiveness could not be reached even at levels as high as 1 microgram/ml. NIH-FSH-P2 and NIAMMD-bLH-4 were considerably less potent than other growth factors. Both preparations were inactive at concentrations less than 1 microgram/ml and produced an optimal response at 10 micrograms/ml.
mesh_terms: D000818:Animals; D002417:Cattle; D002999:Clone Cells / Q000187:drug effects; D003114:Colony-Forming Units Assay; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006065:Gonadotropins, Pituitary / Q000494:pharmacology*; D006107:Granulosa Cells / Q000187:drug effects*; D007986:Luteinizing Hormone / Q000494:pharmacology
chemical_list: D006065:Gonadotropins, Pituitary; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone