The application of fluorescein isothiocyanate and high-performance liquid chromatography for the microsequencing of proteins and peptides.
Amino acid derivatives of fluorescein isothiocyanate have been separated by reverse-phase high-performance liquid chromatography in 45 min, using a linear gradient formed from acetone and 10 mM sodium phosphate buffer (pH 7.0) at 60 degrees C. The fluorescence of the derivatives has been used for detection giving a sensitivity of less than 0.5 pmol for a single component. Applications of this method to the sequence analyses of egg white lysozyme and salmon beta-melanotropin are also described.
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004529:Egg White; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D009074:Melanocyte-Stimulating Hormones; D009113:Muramidase; D010455:Peptides; D011506:Proteins; D012474:Salmon; D013050:Spectrometry, Fluorescence; D013861:Thiocyanates; D013867:Thiohydantoins
D005452:Fluoresceins; D010455:Peptides; D011506:Proteins; D013861:Thiocyanates; D013867:Thiohydantoins; D009074:Melanocyte-Stimulating Hormones; D009113:Muramidase; D016650:Fluorescein-5-isothiocyanate