Isolation and characterization of a frog virus 3 variant resistant to phosphonoacetate: genetic evidence for a virus-specific DNA polymerase.
A variant of frog virus 3 (FV3) resistant to 200 micrograms/ml phosphonoacetate was isolated, and used to establish that the DNA polymerase induced in FV3-infected cells was virus coded. In addition, inhibitor studies showed that the FV3 polymerase is similar to eukaryotic polymerase alpha in its sensitivity to aphidicolin, and that resistance to phosphonoacetate does not confer cross-resistance to thymidine arabinoside or acycloguanosine.
D000818:Animals; D016590:Aphidicolin; D004261:DNA Replication / Q000187:drug effects; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D004224:Diterpenes / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D007497:Iridoviridae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D010746:Phosphonoacetic Acid / Q000494:pharmacology; D011898:Ranidae / Q000382:microbiology; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000187:drug effects
D004224:Diterpenes; D014764:Viral Proteins; D016590:Aphidicolin; D004259:DNA-Directed DNA Polymerase; D010746:Phosphonoacetic Acid