Increase in cell-surface N-acetylglucosaminide beta (1----4)galactosyltransferase activity with retinoic acid-induced differentiation of F9 embryonal carcinoma cells.
Exposure of F9 cells to all-trans-retinoic acid over a period of 6 days resulted in 4-fold induction of cell surface N-acetylglucosaminide beta (1----4)galactosyltransferase (GT) activity. The retinoic acid-induced GT activity was further enhanced by treatment of the cells with 8-bromo cyclic AMP. The ability of retinoic acid alone, or retinoic acid in combination with 8-bromo cyclic AMP, to induce GT activity was inhibited by both actinomycin D and cycloheximide. These findings indicate that the induction of galactosyltransferase activity noted with differentiation of F9 cells involves de novo synthesis of new enzyme protein.
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D054278:Embryonal Carcinoma Stem Cells; D004790:Enzyme Induction / Q000187:drug effects; D005700:Galactosyltransferases / Q000096:biosynthesis*; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000201:enzymology*; D013234:Stem Cells / Q000201:enzymology*; D013724:Teratoma / Q000201:enzymology* / Q000473:pathology; D014212:Tretinoin / Q000494:pharmacology*; D006022:beta-N-Acetylglucosaminylglycopeptide beta-1,4-Galactosyltransferase / Q000096:biosynthesis*
D003609:Dactinomycin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D014212:Tretinoin; D003513:Cycloheximide; D005700:Galactosyltransferases; D006022:beta-N-Acetylglucosaminylglycopeptide beta-1,4-Galactosyltransferase