[Detection of sarcocystosis in heifers and pigs using the indirect immunofluorescence method and trypsinization of muscle tissue].
The incidence of sarcocystosis was studied in cattle and pigs of different age categories by means of the methods of muscular tissue trypsinization and indirect immunofluorescence reaction (IFR). On the whole, 173 head of cattle and 107 pigs were examined. In the category of calves, a positive serological reaction was proved in 75% of cases, and positivity in the trypsinization of muscular tissue was found only in 15% of cases. In the category of beef bulls 64% of cases were serologically positive and 96% were positive in the trypsinization test. In the category of cows 70.84% of cases were positive serologically and 85.42% by trypsinization. In fattened pigs serological positivity was demonstrated in 10.53% of cases but the direct determination of sarcocysts failed in all cases. On the other hand, trypsinization of sows was positive in 8% and IFR was negative in all cases. The discussion deals with the correlations of all results obtained by the above diagnostic methods.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D005455:Fluorescent Antibody Technique; D009132:Muscles / Q000469:parasitology*; D012522:Sarcocystis / Q000276:immunology / Q000302:isolation & purification; D012523:Sarcocystosis / Q000175:diagnosis / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*; D014357:Trypsin
D000906:Antibodies; D014357:Trypsin
Detekce sarkocystózy skotu a prasat metodou neprímé imunofluorescencní reakce a trypsinací svalové tkánĕ.