Large-scale purification of fungal glucoamylases using anion-exchange resin chromatography.
Anion-exchange chromatography on polystyrene resin is shown to be more effective than DEAE-cellulose for purification of glucoamylase from crude enzyme extracts of Aspergillus awamori or from commercial preparations. The glucoamylase from A. awamori culture medium was purified to electrophoretic homogeneity with yields approaching 80%.
D000837:Anion Exchange Resins; D001230:Aspergillus / Q000201:enzymology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005087:Glucan 1,4-alpha-Glucosidase / Q000302:isolation & purification*; D005959:Glucosidases / Q000302:isolation & purification*
D000837:Anion Exchange Resins; D005959:Glucosidases; D005087:Glucan 1,4-alpha-Glucosidase