Vanadate inhibition of the Ca++-ATPase of sarcoplasmic reticulum from pig heart.
Ca++-ATPase of sarcoplasmic reticulum from pig heart can be inhibited by vanadate with half maximal inhibition at about 10(-5)M NH4VO3. At the same time vanadate lowers the [Mg++] for maximal activity of the Ca++-ATPase to half, from 8 X 10(-3)M to 4 X 10(-3)M Mg++. At low vanadate concentrations around 5 X 10(-8)M the Ca++-ATPase was activated.
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006321:Heart / Q000187:drug effects; D009206:Myocardium / Q000201:enzymology; D012519:Sarcoplasmic Reticulum / Q000187:drug effects* / Q000201:enzymology; D013552:Swine; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology*
D014639:Vanadium; D014638:Vanadates; D000252:Calcium-Transporting ATPases