Localization of three inhibitors of cysteineproteinases in the human oral mucosa.
3 known human cysteineproteinase inhibitors--neutral cysteineproteinase inhibitor (NCPI), acid cysteineproteinase inhibitor (ACPI) and serum alpha-cysteineproteinase inhibitor (alpha-CPI)--were localized immunohistochemically in the squamous epithelium of the mouth by using the peroxidase-antiperoxidase complex method and formaldehyde-fixed paraffin-embedded tissue sections. Neither ACPI nor NCPI was found in the 2 to 3 most basal cell layers of the epithelium, but a strong cytoplasmic reaction was seen in the upper cell layers by using either anti-ACPI or anti-NCPI serum. In some cells the most dense stain precipitate was seen around or in the nucleus, and in the highest cell layers the staining was sometimes associated with the cell membrane. alpha-CPI was localized mainly in the epithelial stroma and in the intercellular space of the epithelium.
D015853:Cysteine Proteinase Inhibitors; D003593:Cytoplasm / Q000032:analysis; D004848:Epithelium / Q000032:analysis; D006651:Histocytochemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007120:Immunochemistry; D009061:Mouth Mucosa / Q000032:analysis* / Q000166:cytology; D011480:Protease Inhibitors / Q000032:analysis*; D011506:Proteins / Q000032:analysis*
D015853:Cysteine Proteinase Inhibitors; D011480:Protease Inhibitors; D011506:Proteins