Comparative studies on different methods in typing strains of erysipelothrix rhusiopathiae. I. Methods and influence of some factors on their results.
To contribute to the standardization of typing Erysipelothrix rhusiopathiae strains the following points have been examined and compared: the usefulness of tube precipitation, paper chromatography and agar gel diffusion; different methods to produce good antisera (specific high titres) and antigens (CH3COOH-, HCl-extract, extraction by means of autoclavation). Rabbits have a considerably varying individual ability to produce Erysipelas antibodies with good precipitation titres. The antigenic effect of different E.rh.-strains and of dissociated forms is not equal. The density of bacteria suspension for extract production and for rabbit immunization has to be sufficient. The period of usability of extracts and antisera is different.
D000372:Agglutination Tests; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D004888:Erysipelothrix / Q000145:classification* / Q000254:growth & development; D007106:Immune Sera; D007114:Immunization; D005779:Immunodiffusion; D011817:Rabbits; D012703:Serotyping / Q000379:methods*
D000942:Antigens, Bacterial; D007106:Immune Sera