title: High ratio of alkali-sensitive lesions to total DNA modification induced by benzo(a)pyrene diol epoxide.
abstract: The ratio of alkali-labile lesions to total DNA adducts for DNA modified by an active metabolite of benzo(a)pyrene was investigated using DNA sequencing methodology. About 40% of the adducts formed result in alkali-labile sites. About 25% of the lesions were alkali-labile at positions of guanine, 10% at adenine, and 5% at cytosine. This study highlights the potential role of adducts other than the N2-substituted guanine in mutagenic and carcinogenic effects of benzo(a)pyrene.
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D001482:Base Composition; D001483:Base Sequence; D001580:Benzopyrenes; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004279:DNA, Viral; D006863:Hydrogen-Ion Concentration; D007700:Kinetics
chemical_list: D001580:Benzopyrenes; D004279:DNA, Viral; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide