title: Decalcification for electron microscopy with L-ascorbic acid.
abstract: L-Ascorbic acid decalcification was used for electron microscopy of mammalian tooth germs and bone after fixation in a glutaraldehyde-paraformaldehyde mixture. The recommended decalcifying solution is 2% with respect to L-ascorbic acid and 0.9% with respect to sodium chloride. The method has the advantage that decalcification is complete within a quarter of the time required with EDTA. The fine structure of ameloblasts and hard tissue is preserved as well as with EDTA.
mesh_terms: D000565:Ameloblasts / Q000648:ultrastructure; D000818:Animals; D001205:Ascorbic Acid; D003648:Decalcification Technique; D004492:Edetic Acid; D007568:Jaw / Q000648:ultrastructure*; D008854:Microscopy, Electron; D051381:Rats; D014083:Tooth Germ / Q000648:ultrastructure*
chemical_list: D004492:Edetic Acid; D001205:Ascorbic Acid