Isolation and partial characterization of collagen fibrils, fibers and fiber-bundles from insoluble calf dermis.
Collagen fibrils, fibers and fiber bundles have been isolated from insoluble calf skin chips by disaggregation with a neutral salt buffer followed by differential centrifugation. The ratio of type I collagen/type III collagen was relatively constant, regardless of the size of the fibrous materials. On the other hand, the ratios of glycosaminoglycans/collagen and non-collagenous glycoproteins/collagen were significantly higher in the thinner fiber fractions. However, the ratio of hyaluronic acid to dermatan sulfate was unchanged. The role of the non-collanenous constituents in collagen fibrillogenesis in vivo is discussed.
D000596:Amino Acids / Q000032:analysis; D000606:Amino Sugars / Q000302:isolation & purification; D000818:Animals; D002417:Cattle; D002498:Centrifugation; D003094:Collagen / Q000032:analysis* / Q000378:metabolism; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006025:Glycosaminoglycans / Q000302:isolation & purification; D008854:Microscopy, Electron; D012867:Skin / Q000032:analysis*
D000596:Amino Acids; D000606:Amino Sugars; D006025:Glycosaminoglycans; D003094:Collagen; D005947:Glucose; D005690:Galactose