title: Methyl-coenzyme M, an intermediate in methanogenic dissimilation of C1 compounds by Methanosarcina barkeri.
abstract: Extracts of Methanosarcina barkeri possess a specific methyltransferase that catalyzes the transfer of the methyl group of methanol to 2-mercaptoethanesulfonic acid. Over a fourfold range in added 2-mercaptoethanesulfonic acid, the formation of 2-(methylthio)ethanesulfonic acid exhibited a 1:1 ratio to 2-mercaptoethanesulfonic acid added. This reaction required adenosine 5'-triphosphate; a maximal ratio (mole/mole) of 85 methyl groups was transferred per adenosine 5'-triphosphate added. The methyltransferase was found in extracts of methanol-grown cells as well as in extracts of hydrogen-grown cells. In extracts of cells grown on either substrate, 2-(methylthio)ethanesulfonic acid was formed from added methanol or methylamine but not from acetate.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D002474:Cell-Free System; D002614:Chelating Agents / Q000494:pharmacology; D019605:Euryarchaeota / Q000378:metabolism*; D008623:Mercaptoethanol / Q000031:analogs & derivatives*; D015080:Mesna / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism; D008697:Methane / Q000096:biosynthesis*; D000432:Methanol / Q000378:metabolism; D008744:Methylamines / Q000378:metabolism; D008780:Methyltransferases / Q000378:metabolism
chemical_list: D002614:Chelating Agents; D008744:Methylamines; C023349:methyl coenzyme M; D008623:Mercaptoethanol; D000255:Adenosine Triphosphate; D008780:Methyltransferases; D000251:Adenosine Triphosphatases; D015080:Mesna; D008697:Methane; D000432:Methanol