Characterization of a carotenoprotein from the carapace of the crab Macropipus puber.
An orange carotenoprotein (lambda max = 480 nm) containing astaxanthin as prosthetic group was extracted and purified from the carapace of the crab Macropipus puber. The extraction was made possible by means of Triton X-100, yielding an orange carotenoprotein, with a molecular weight of about 56,000. Sodium dodecyl sulfate polyacrylamide gel electrophoresis indicated only a single polypeptide of 14,000 daltons, suggesting that the orange caroteno-protein was a tetrameric form.
D000818:Animals; D003386:Brachyura / Q000032:analysis*; D002338:Carotenoids / Q000032:analysis* / Q000302:isolation & purification; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008055:Lipids / Q000032:analysis; D008970:Molecular Weight; D010860:Pigments, Biological / Q000032:analysis* / Q000302:isolation & purification; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D013053:Spectrophotometry; D024341:Xanthophylls; D019207:beta Carotene / Q000031:analogs & derivatives*
D008055:Lipids; D010860:Pigments, Biological; D011506:Proteins; D024341:Xanthophylls; D019207:beta Carotene; D002338:Carotenoids; C005948:astaxanthine