Fluorescence polarization immunoassay of estradiol.
Fluorescence polarization immunoassay (FPIA) offers a good alternative to isotopic methods for the determination of drug and hormone levels in biological fluids. We applied it to estradiol, using a fluoresceinated derivative of estradiol and the IgG fraction of a highly specific rabbit antiserum. After determining the optimal operational conditions, FPIA was used for measuring urinary concentrations of free estradiol in normal and pregnant women, after a purification step on a reversed phase cartridge. The method turned out to be rapid (1 min to reach the equilibrium state) and accurate.
D000818:Animals; D004958:Estradiol / Q000032:analysis* / Q000276:immunology / Q000652:urine; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005454:Fluorescence Polarization / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D011247:Pregnancy; D011817:Rabbits; D013861:Thiocyanates
D005452:Fluoresceins; D013861:Thiocyanates; D004958:Estradiol; D016650:Fluorescein-5-isothiocyanate