Comparison of detergent versus protease solubilized rat intestinal lactase.
Comparisons between papain- and Triton X-100-solubilized lactase (EC 3.2.1.23) were made in terms of elution from various chromatographic columns and by molecular weight determinations. Using these techniques, no major differences could be detected. Since papain-solubilized enzyme would be cleaved at the hydrophilic-hydrophobic interface and detergent would release the amphipathic enzyme, the lack of detectable differences between purified lactase solubilized by the two agents suggests the existence of a relatively small anchoring moiety in rats when compared to that suggested in previous studies for adult humans.
D000818:Animals; D003902:Detergents / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005696:Galactosidases / Q000302:isolation & purification*; D007422:Intestines / Q000201:enzymology*; D008970:Molecular Weight; D017830:Octoxynol; D010206:Papain / Q000494:pharmacology; D010447:Peptide Hydrolases / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D012995:Solubility; D013501:Surface-Active Agents / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000302:isolation & purification*
D003902:Detergents; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D017830:Octoxynol; D005696:Galactosidases; D001616:beta-Galactosidase; D010447:Peptide Hydrolases; D010206:Papain