Elimination of germ-line tandemly repeated sequences from the somatic genome of the ciliate Oxytricha fallax.
The ciliated protozoa exhibit nuclear dimorphism. The genome of the somatic macronucleus arises from the germ-line genome of the micronucleus following conjugation. We have studied the fates of highly repetitious sequences in this process. Two cloned, tandemly repeated sequences from the micronucleus of Oxytricha fallax were used as probes in hybridizations to micronuclear and macronuclear DNA. The results of these experiments show: (1) the cloned repeats are members of two apparently unrelated repetitious sequence families, which each appear to comprise a few percent of the micronuclear genome, and (2) the amount of either family in the macronuclei from which our DNA was prepared is about 1/15 that found in an equal number of diploid micronuclei. Most, if not all, of the apparent macronuclear copies of these repeats can be accounted for by micronuclear contamination, which strongly suggests that these sequences are eliminated from the macronuclei and have no vegetative function.
D002467:Cell Nucleus / Q000648:ultrastructure; D016798:Ciliophora / Q000235:genetics*; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid