A new method for in vitro chemosensitivity assay: inhibition of metabolic CO2 production.
This report describes a new method for in vitro chemosensitivity testing based on the inhibition by chemotherapeutic agents of 14CO2 production by human malignant tumor cells incubated with 14C-glucose. The application of the method to the inhibition of the metabolism of a continuous cell line of human breast carcinoma origin by various concentrations of doxorubicin is illustrated. The degree of inhibition is demonstrated to be a function of the drug concentration.
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D002460:Cell Line; D004317:Doxorubicin / Q000494:pharmacology; D006801:Humans; D013997:Time Factors
D000970:Antineoplastic Agents; D002245:Carbon Dioxide; D004317:Doxorubicin