title: Proline transport increases growth efficiency in salt-stressed Streptomyces griseus.
abstract: Streptomyces griseus synthesizes proline for osmoregulation under salt stress. Uptake of exogenous [14C]proline and internal synthesis of proline were quantified in cells growing at salt concentrations from 0 to 1 M NaCl. Externally supplied proline accounted for an increased proportion of the intracellular pool of free proline as salt concentration was increased, but neither the concentration nor the composition of the internal amino acid pool was substantially altered by supply of exogenous proline. Uptake of exogenous proline significantly increased the specific growth yield of S. griseus growing under salt stress; the increased yield was proportional to reductions in proline synthesis.
mesh_terms: D001692:Biological Transport / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D011392:Proline / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*; D012988:Soil Microbiology; D013305:Streptomyces griseus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D014882:Water-Electrolyte Balance / Q000187:drug effects
chemical_list: D012965:Sodium Chloride; D011392:Proline