[In vitro method determining sensitivity of anticancer agents by incorporation of radioactive precursors].
A new sensitivity test of anticancer agents was developed to measure the lethal effects of cancer cells by the incorporation of radioactive precursors. The thousand cancer cells were cultured in a microplate in the presence of anticancer agents. These cells were exposed to radioactive precursors. Two or three days later, the cancer cells were harvested on a glass fiber filter by a multiple automatic cell-harvester and the incorporation of precursors was counted by a liquid scintillation counter. In this study, the in vivo results of drug testing in animal model systems were compared with drug sensitivities. Mice inoculated Ehrlich ascites cells were treated with various kinds of anticancer drugs. The development of the cells was compatible with the result of the sensitivity test. The growths of Lauson and ME-180 cells derived from human cancers implanted subcutaneously to nude mice were also well correlated with this sensitivity test.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D004341:Drug Evaluation / Q000379:methods*; D004351:Drug Resistance; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008819:Mice, Nude; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D012517:Sarcoma, Yoshida / Q000378:metabolism; D013997:Time Factors; D014316:Tritium / Q000378:metabolism
D000970:Antineoplastic Agents; D008937:Mitomycins; D014316:Tritium; D016685:Mitomycin