A solid-phase chemiluminescence immunoassay for determination of IgG in eluates of psoriatic scales.
A solid-phase chemiluminescence immunoassay for determining immunoglobulin G (IgG) in eluates of psoriatic scales is described. Polystyrene balls coated with protein A bound the IgG present in the eluates and in human sera. The bound IgG was then detected by a luminol-coupled goat anti-human IgG conjugate, characterized for antibody, protein and luminol content. This method correlated well with radial immunodiffusion in determining IgG levels in human sera (r = 0.97). The sensitivity of the assay was 0.5 micrograms IgG. Eluates prepared from psoriatic scales treated sequentially with buffers of decreasing pH all contained IgG when tested by this method. pH 2 eluates, however, contained more IgG than pH 3 eluates, suggesting the presence of specifically bound antibodies.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D004848:Epithelium / Q000276:immunology; D006041:Goats; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods*; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D008163:Luminescent Measurements; D008165:Luminol; D008863:Microspheres; D011565:Psoriasis / Q000276:immunology* / Q000473:pathology
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D008165:Luminol