Cloning of the histidine transport genes from Salmonella typhimurium and characterization of an analogous transport system in Escherichia coli.
The genes for the well-characterized high-affinity histidine transport system of S typhimurium have been cloned in lambda gt4. Genetic and physiological analyses of the analogous transport system of E coli were undertaken in order that available lambda vectors, recombinant DNA techniques, and a genetic selection for transport function might be used to isolate the Salmonella genes. The presence of the transport genes on a 12.4 Kb cloned DNA fragment has been confirmed 1) genetically, by complementation studies; 2) physiologically, by the rates of histidine uptake by bacteria containing this DNA; and 3) by demonstrating that the cloned DNA codes for the previously identified transport proteins J and P. The isolated fragment carries the entire transport operon, the argT gene and the ubiX locus, but neither the purF gene nor the ack/pta loci.
D010582:Bacteriophage lambda / Q000378:metabolism; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism*; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005796:Genes; D005816:Genetic Complementation Test; D005838:Genotype; D006639:Histidine / Q000378:metabolism*; D009876:Operon; D033902:Periplasmic Binding Proteins; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity
D002352:Carrier Proteins; D004274:DNA, Recombinant; D033902:Periplasmic Binding Proteins; C018963:histidine-binding protein; D006639:Histidine