Induction of cytolytic cells by pure recombinant human interleukin 2.
Purified recombinant human interleukin 2, produced in Escherichia coli, was sufficient to generate cytolytic cells in concanavalin A-stimulated, T helper cell-depleted (Lyt-1.1-) or accessory cell-depleted (Ia-) murine spleen cell cultures. Moreover, recombinant interleukin 2 (rIL2) was able (even at 0.5 ng/ml) to induce cytolytic cells in undepleted murine spleen cell cultures and undepleted human peripheral blood lymphocyte cultures in the absence of a mitogen. Purified recombinant human interferon-gamma, produced in Chinese hamster ovary cells, did not induce either cytolytic activity or IL2 responsiveness in human peripheral blood lymphocyte cultures. Possibly, the rIL2-induced cytolytic cells are formed as a result of nonspecific secondary cytotoxic T lymphocyte activation.
D000818:Animals; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000008:administration & dosage* / Q000235:genetics; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D010957:Plasmids; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology* / Q000276:immunology
D007376:Interleukin-2; D007371:Interferon-gamma