Successful intracerebral allotransplantation of pancreatic endocrine cells in spontaneous diabetic BB rats without immunosuppression.
An approach for pancreatic-islet transplantation in the spontaneous diabetic Bio-Breeding (BB) Wistar rat is described in which purified allogeneic pancreatic endocrine cells (PEC) are inoculated intracerebrally in the diabetic recipients. Without immunosuppression, 12/12 of the recipient animals responded to the transplanted PEC with no further need of insulin administration. Also, 8/12 showed normalization of nonfasting blood glucose (BG), 24-hour urine output, and gradual increase in body weight (BW) for a mean observation period of over 122.1 +/- 13.6 days. They had a glucose disappearance K rate of 1.7 +/- 0.3 (mean +/- SE), following intravenous (IV) glucose load. The grafted PEC remain functional and apparently are not affected by the usual immunorejection and/or original disease process during the whole period of observation. This achievement of long-term allograft function without immunosuppression would provide an opportunity to study the recurrence of diabetes and the effect of islet-cell transplantation in chronic diabetic complication in the spontaneous diabetic BB rat model which closely resembles human type 1 diabetes mellitus.
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001921:Brain / Q000601:surgery; D003920:Diabetes Mellitus / Q000378:metabolism / Q000628:therapy* / Q000662:veterinary; D004195:Disease Models, Animal; D004231:Diuresis; D005260:Female; D007165:Immunosuppression Therapy; D007328:Insulin / Q000008:administration & dosage; D016381:Islets of Langerhans Transplantation; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012376:Rodent Diseases / Q000378:metabolism
D001786:Blood Glucose; D007328:Insulin