Isolated chromaffin cells from adrenal medulla contain primarily monoamine oxidase B.
Cultured chromaffin cells from bovine adrenal medulla were found to contain primarily the B form of monoamine oxidase. This monoamine oxidase B enzyme was somewhat distinct from B enzymes from other sources, in that noradrenaline was a much poorer substrate than serotonin. Nonetheless, studies with selective inhibitors of the A form (clorgyline) and the B form [(-)-deprenyl] confirmed that chromaffin cell monoamine oxidase was the B form. The observation that chromaffin cell monoamine oxidase has poor affinity for catecholamines is consistent with physiological needs that require the cell to synthesize and store large amounts of catecholamines.
D000313:Adrenal Medulla / Q000166:cytology* / Q000201:enzymology; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D002395:Catecholamines / Q000378:metabolism; D002417:Cattle; D002838:Chromaffin System / Q000201:enzymology*; D003010:Clorgyline / Q000494:pharmacology; D006801:Humans; D008995:Monoamine Oxidase / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism; D012642:Selegiline / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism
D002395:Catecholamines; D012642:Selegiline; D012701:Serotonin; D008995:Monoamine Oxidase; D003010:Clorgyline; D009638:Norepinephrine