Neutrophil labeling: problems and pitfalls.
The use of neutrophils labeled with gamma-emitting radionuclides has been shown to be acceptable for in vivo kinetic studies as well as for imaging inflammatory foci. Among the gamma-emitting radionuclides, indium-111 appears to be the agent of choice. Labeling neutrophils with 111In, however, is a relatively new technique. Although simple to perform, it involves several stages, none of which could be carried out without problems. These are discussed and the current research aimed at eliminating the problems is outlined. The knowledge of specific chemotactic receptors and surface antigens has stimulated investigations into selective neutrophil labeling that will continue to be challenging and exciting.
D000818:Animals; D002469:Cell Separation / Q000379:methods; D002860:Chromium Radioisotopes; D004285:Dogs; D005710:Gallium Radioisotopes; D006801:Humans; D007204:Indium; D007553:Isotope Labeling / Q000379:methods*; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils; D009942:Organometallic Compounds; D015125:Oxyquinoline / Q000031:analogs & derivatives; D010761:Phosphorus Radioisotopes; D011725:Pyridines; D011786:Quality Control; D011829:Radiation Dosage; D011868:Radioisotopes; D012645:Selenomethionine; D013455:Sulfur; D013667:Technetium; D013671:Technetium Tc 99m Sulfur Colloid; D013871:Thiones
D002860:Chromium Radioisotopes; D005710:Gallium Radioisotopes; D009942:Organometallic Compounds; D010761:Phosphorus Radioisotopes; D011725:Pyridines; D011868:Radioisotopes; D013871:Thiones; D007204:Indium; C032950:indium oxine; D013671:Technetium Tc 99m Sulfur Colloid; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015125:Oxyquinoline; C008704:pyrithione; D013455:Sulfur; D013667:Technetium; D012645:Selenomethionine