Neutralization of croatalidae venom induced platelet aggregation by affinity chromatography isolated IgG to Crotalus viridis helleri venom.
IgG was isolated from commercial antivenin by affinity chromatography using Crotalus viridis helleri venom as an affinant. This purified IgG was demonstrated to be more effective than the commercial antivenin in preventing platelet aggregation induced by four separate crotalid venoms in vitro. Affinity chromatography appears to be an effective means for specific, polyvalent IgG isolation and purification.
D000818:Animals; D000997:Antivenins / Q000494:pharmacology*; D001797:Blood Protein Electrophoresis; D002846:Chromatography, Affinity; D003435:Crotalid Venoms / Q000633:toxicity*; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000302:isolation & purification*; D066298:In Vitro Techniques; D010974:Platelet Aggregation / Q000187:drug effects*; D012911:Snakes
D000997:Antivenins; D003435:Crotalid Venoms; D007074:Immunoglobulin G