title: Bacteriological examination of specified-pathogen-free animals: a procedure for small rodents.
abstract: Organs of 10 mice or 5 rats were pooled and homogenized. A portion of the homogenate was cultured on several selective and enrichment media. The method was tested by adding various pathogenic organisms to organ homogenates. 10(3)-10(4) microorganisms in homogenates of 10 organs were readily detected. This procedure allows even a small microbiological laboratory to detect a low frequency of infected animals in a colony.
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D003470:Culture Media; D005856:Germ-Free Life; D010325:Pasteurella / Q000302:isolation & purification; D012377:Rodentia / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000302:isolation & purification; D013047:Specific Pathogen-Free Organisms
chemical_list: D003470:Culture Media