Analysis of commercial pollen extracts by enzyme determination. II. Determination of the contamination of pollen extracts with plant matter.
The contamination of pollen extracts by plant matter was studied by means of enzymes. Beta-glucosidase was detected only in flowers and leaves by using the Api-Zym System. 29 pollen extracts were prepared after microscopic control. Among these 23 did not contain any plant matter and no beta-glucosidase activity could be detected. In contrast, 6 extracts were contaminated with plant matter and in every case beta-glucosidase activity was found 34 commercial pollen extracts completed the study. beta-glucosidase activity was present in 8 of them. It may be postulated that glucosidase activity detected using 2- naphthyl - beta D-glucopyranoside as a substrate, can be correlated with the presence of plant matter from the raw material from which the extract was prepared. This contamination may be important to determine since some additional sensitization may occur with such extracts during immunotherapy.
D004340:Drug Contamination; D010936:Plant Extracts / Q000032:analysis*; D010940:Plant Proteins / Q000032:analysis; D011058:Pollen / Q000032:analysis* / Q000201:enzymology / Q000648:ultrastructure; D001617:beta-Glucosidase / Q000032:analysis
D010936:Plant Extracts; D010940:Plant Proteins; D001617:beta-Glucosidase