NADH-specific dihydropteridine reductase in mastocytoma P-815 cells.
NADH-specific dihydropteridine reductase [EC 1.6.99.7] was purified from mouse mastocytoma P-815 cells. Km values for NADH and NADPH were determined to be 1.4 microM and 32 microM, respectively, using tetrahydro-6-methylpterin. Molecular weight was 50,000, and subunit molecular weight was 25,000. The enzymes from P-815 and liver of host mouse (DBA/2) showed similar electrophoretic mobility on polyacrylamide gel. The P-815 enzyme reacted with antiserum against bovine liver NADH-specific dihydropteridine reductase, forming a single precipitin line.
D000818:Animals; D002417:Cattle; D002460:Cell Line; D004093:Dihydropteridine Reductase / Q000302:isolation & purification* / Q000378:metabolism; D012515:Mast-Cell Sarcoma / Q000201:enzymology*; D051379:Mice; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000302:isolation & purification*; D009363:Neoplasm Proteins / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D013045:Species Specificity; D013379:Substrate Specificity; D014365:Tryptophan Hydroxylase / Q000032:analysis
D009363:Neoplasm Proteins; D009243:NAD; D014365:Tryptophan Hydroxylase; D004093:Dihydropteridine Reductase; D009247:NADH, NADPH Oxidoreductases