Blood granulocyte-macrophage progenitor cell concentrations and differentiation in vitro in patients with hepatic cirrhosis.
Blood granulocyte-macrophage progenitor cells (CFU-GM) and myeloid differentiation were studied in 16 patients with hepatic cirrhosis by culturing blood mononuclear cells in semisolid and in liquid medium. The numbers of CFU-GM from normal and cirrhotic subjects were not significantly different, even when increased numbers of monocyte-macrophagic colonies and decreased numbers of granulocytic colonies were observed in cirrhotic patients. Significantly reduced granulocytic growth and increased monocyte-macrophagic cell growth were found in liquid culture of cirrhotic patients. These data seem to indicate that in hepatic cirrhosis, besides granulocyte sequestration within the spleen, there is a disorder of granulocytic versus monocyte-macrophagic differentiation.
D000328:Adult; D000362:Agar; D000368:Aged; D002452:Cell Count; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D006098:Granulocytes; D006412:Hematopoietic Stem Cells; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood*; D008264:Macrophages; D008297:Male; D008875:Middle Aged
D003470:Culture Media; D000362:Agar