Commitment to erythroid or granulocyte-macrophage differentiation is associated with loss of macromolecular insoluble cold globulin.
In mice a macromolecular insoluble cold globulin (MICG) is present on the surface of resting and stimulated peripheral T lymphocytes, thymocytes, fetal prothymocytes and hemopoietic stem cells forming spleen colonies, colony-forming unit-spleen (CFU-S). Here we demonstrate that removal of MICG positive cells from bone marrow by treatment with antibody and complement does not affect the number of erythroid, burst-forming unit-erythroid (BFU-E) and granulocyte-macrophage, colony-forming unit-granulocyte macrophage (CFU-GM) progenitors developing in vitro. Thus, commitment of stem cells to lineage specific differentiation is associated with the loss of the MICG marker. Furthermore, the removal of MICG positive T lymphocytes from bone marrow does not affect the growth of BFU-E or CFU-GM.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D002454:Cell Differentiation; D003450:Cryoglobulins / Q000032:analysis*; D004912:Erythrocytes / Q000166:cytology; D005260:Female; D006098:Granulocytes / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000032:analysis; D007962:Leukocytes / Q000166:cytology; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008808:Mice, Inbred CBA; D012995:Solubility; D013950:Thymus Gland / Q000166:cytology
D003450:Cryoglobulins