Synthesis of thymine and alpha-putrescinylthymine in bacteriophage phi W-14-infected Pseudomonas acidovorans.
Host DNA synthesis stopped about 10 min after the infection of Pseudomonas acidovorans with bacteriophage phi W-14, but host DNA was not degraded to acid-soluble fragments. The synthesis of host but not of phage DNA was inhibited by 5-fluorodeoxyuridine. The nucleotide pools of infected cells did not contain dTTP, and infection resulted in the appearance of dTTPase activity. Although ornithine labeled the alpha-putrescinylthymine residues of phi W-14 DNA, ornithine-labeled nucleotides were not detected in infected cells. A new deoxynucleoside triphosphate did appear in infected cells, but it was not labeled by ornithine. It is concluded that the thymine and alpha-putrescinylthymine in phi W-14 DNA are synthesized at the polynucleotide level.
D001435:Bacteriophages / Q000254:growth & development / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis; D005467:Floxuridine / Q000494:pharmacology; D011549:Pseudomonas / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D013941:Thymine / Q000031:analogs & derivatives* / Q000096:biosynthesis*; D013942:Thymine Nucleotides / Q000378:metabolism
D004269:DNA, Bacterial; D004279:DNA, Viral; D013942:Thymine Nucleotides; D005467:Floxuridine; C025113:alpha-putrescinylthymine; D013941:Thymine; D013936:Thymidine