Correlation of interferon gamma inducing ability to sugar binding specificities of various lectins.
Various lectins were examined to determine possible induction of gamma interferon (IFN-gamma) in human leukocytes. Among the seven positive lectins (Concanavalin A, pea lectin, lentil lectin, rice bran agglutinin, pokeweed mitogen, wheat germ agglutinin, phytohemagglutinin-P), six except rice bran agglutinin belonged to those which recognize carbohydrate chains connected to polypeptide through a glycosylamine linkage between N-acetylglucosamine (GlcNAc) and asparagine residues. The specificity of carbohydrate chain recognition of rice bran agglutinin, residual one positive lectin, has not been reported. Induction of IFN-gamma by wheat germ agglutinin, one of the positive lectins, was inhibited by the addition of GlcNAc during the induction, but not by the addition of glucose, galactose, alpha-methylmannose, N-acetylgalactosamine, N-acetylmannosamine, and lactose.
D001665:Binding Sites; D002241:Carbohydrates; D006801:Humans; D007369:Interferon Inducers; D007371:Interferon-gamma / Q000096:biosynthesis*; D007700:Kinetics; D037102:Lectins / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology*; D011485:Protein Binding
D002241:Carbohydrates; D007369:Interferon Inducers; D037102:Lectins; D007371:Interferon-gamma