Cytochemical detection of erythroblastic enzymes in acquired dyserythropoiesis.
We have studied the amount and intracellular distribution of erythroblastic alkaline and acid phosphatase, nonspecific esterase, and N-acetyl-beta-glucosaminidase in 50 patients with acquired dyserythropoiesis. 19 morphologically normal bone marrow smears served as controls. Alkaline phosphatase was found in all controls (mean 9.4% of erythroblasts). The percentage of positive erythroblasts in pathological conditions varied greatly from absence to over 70%. Acid phosphatase and N-acetyl-beta-glucosaminidase were positive in pernicious anaemia and in acute erythremic myelosis. Nonspecific esterase was only detected in a case of erythremic myelosis. This case also showed a faint metachromasia with the dye azure A.
D000118:Acetylglucosaminidase / Q000097:blood; D000135:Acid Phosphatase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000741:Anemia, Aplastic / Q000201:enzymology*; D004900:Erythroblasts / Q000201:enzymology*; D004950:Esterases / Q000097:blood; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology*
D004950:Esterases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000118:Acetylglucosaminidase