title: [Radioimmunoassay of dehydroepiandrosterone and 5-androsten-3 beta, 17 beta-diol (author's transl)].
abstract: A radioimmunological method is described for the determination of dehydroepiandrosterone and 5-androsten-3 beta, 17 beta-diol in plasma. Interfering cross-reactions of the antiserum of 5-androsten-3 beta, 17 beta-diol with testosterone and pregnenolone make it necessary to separate the steroids by thin-layer chromatography before their final determination. With respect to sensitivity, this method is superior to the commonly used photometric and gas-chromatographic analytical procedures. For both steroids the limits of detection are about 170 fmol.
mesh_terms: D015114:Androstenediol / Q000097:blood*; D000734:Androstenediols / Q000097:blood*; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003687:Dehydroepiandrosterone / Q000097:blood*; D006801:Humans; D010783:Photometry; D011284:Pregnenolone / Q000097:blood; D011863:Radioimmunoassay / Q000379:methods; D013739:Testosterone / Q000097:blood
chemical_list: D000734:Androstenediols; D013739:Testosterone; D003687:Dehydroepiandrosterone; D011284:Pregnenolone; D015114:Androstenediol
vernacular_title: Radioimmunologische Bestimmungsmethoden für Dehydroepiandrosteron und 5-Androsten-3 beta, 17 beta-diol.