Isolation and characterization of sulphated mucopolysaccharides from rat leukaemic (RBL-1) basophils.
Proteoglycans of 300 000 mol.wt. were isolated from dispersed rat basophil tumour cells after labelling of the sulphated mucopolysaccharides with 35S in vitro:90% of the 35S-labelled mucopolysaccharides were extracted at high salt concentration. Alkali degradation of the 35S-labelled proteoglycans yielded glycosaminoglycan chains of 40 000 mol.wt. The composition of the salt-extracted 35S-labelled mucopolysaccharides, as defined by parallel or sequential degradation with chondroitinase AC, chondroitinase ABC and heparinase and resolution of the disaccharide-digestion products obtained with chondroitinase AC, was 48--61% chondroitin 4-sulphate, 20--30% dermatan sulphate, 10--15% heparin and 7--9% chondroitin 6-sulphate. Most of the salt-extracted 35S-labelled mucopolysaccharides were highly charged, with heparin and chondroitin 6-sulphate being relatively uniform in this regard, whereas chondroitin 4-sulphate and dematan sulphate exhibited a range of charge characteristics. The diversity of sulphated mucopolysaccharides present in the rat leukaemic basophil is in contrast with the predominance of heparin in the rat mast cell.
D000818:Animals; D001491:Basophils / Q000032:analysis*; D002807:Chondroitin / Q000032:analysis; D002810:Chondroitinases and Chondroitin Lyases; D002850:Chromatography, Gel; D006025:Glycosaminoglycans / Q000302:isolation & purification*; D006493:Heparin / Q000032:analysis; D007938:Leukemia / Q000032:analysis*; D051381:Rats; D013462:Sulfur Radioisotopes
D006025:Glycosaminoglycans; D013462:Sulfur Radioisotopes; D006493:Heparin; D002807:Chondroitin; D002810:Chondroitinases and Chondroitin Lyases