title: Electrical response of beef-heart submitochondrial particles bound to phospholipid-impregnated millipore filters during ATP hydrolysis.
abstract: 1. Beef heart submitochondrial particles bound to asolectin impregnated Millipore filter, according to the method described earlier (Drachev et al. (1974) Nature 249, 321--324), are able to generate, upon addition of ATP, an electrical potential which can be directly measured. 2. The transmembrane electrical potential generated by ATP hydrolysis reaches values up to 80 mV. The half-time required to attain the plateau of potential is paradoxically long (5 to 10 min at room temperature) and is temperature-dependent. Among different phospholipid species which have been used to impregnate the Millipore filter, phosphatidylethanolamine was found to be the most effective for generation of electrical potential. 3. The potential generated by ATP hydrolysis is inhibited by inhibitors of mitochondrial ATPase, by the uncoupler FCCP and by reagents collapsing the membrane potential. 4. Addition of inhibitors of mitochondrial ATPase, when the plateau of potential is attained, results in a decay of potential. This decay of potential is as slow as the generation of potential induced by ATP hydrolysis. 5. The initial rise in electrical potential is proportional to the ATPase activity.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008564:Membrane Potentials; D008928:Mitochondria / Q000502:physiology*; D008929:Mitochondria, Heart / Q000187:drug effects / Q000502:physiology*; D009840:Oligomycins / Q000494:pharmacology; D010743:Phospholipids / Q000494:pharmacology*; D013367:Submitochondrial Particles / Q000187:drug effects / Q000502:physiology*; D013696:Temperature
chemical_list: D009840:Oligomycins; D010743:Phospholipids; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases