Incorporation of isotope from specifically labeled glucose into alginates of Pseudomonas aeruginosa and Azotobacter vinelandii.
The incorporation of isotope from [6-14C]glucose into alginate by both Pseudomonas aeruginosa and Azotobacter vinelandii was 10-fold greater than that from either [1-14C]- or [2-14C]glucose, indicating preferential utilization of the bottom half of the glucose molecule for alginate synthesis. These data strongly suggest that the Entner - Doudoroff pathway plays a major role in alginate synthesis in both P. aeruginosa and A. vinelandii.
D000464:Alginates / Q000378:metabolism*; D001395:Azotobacter / Q000378:metabolism*; D002250:Carbon Radioisotopes; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D013045:Species Specificity
D000464:Alginates; D002250:Carbon Radioisotopes; D005947:Glucose