Mechanism of inhibition of natural killing by a glycopeptide isolated from the K562 plasma membrane.
The mechanism of lysis by cytotoxic T lymphocytes, K cells, and natural killer (NK) cells is imperfectly understood at this point. In this report, material (glycopeptide) isolated from the plasma membranes of K562 cells and fractionated on lectin affinity adsorbents which has been shown to inhibit NK lysis, was used in several specific NK assays to ascertain what stages of the NK-lytic sequence is inhibited by this substance. Results indicate that this glycopeptide (a) does not inhibit initial binding, but dissociates conjugates following initial effector target interactions; (b) inhibits NK lysis beyond Ca-dependent programming, and (c) inhibits lysis induced by NK cell-derived soluble cytotoxic factors (NKCF) in a soluble factor assay. These results suggest that this glycopeptide can effect the lethal hit stage of NK lysis and may represent structures which can associate directly with NKCF.
D001667:Binding, Competitive; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004492:Edetic Acid / Q000494:pharmacology; D006020:Glycopeptides / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D055646:Killer Factors, Yeast; D008565:Membrane Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*
D006020:Glycopeptides; D055646:Killer Factors, Yeast; D008565:Membrane Proteins; D011506:Proteins; D004492:Edetic Acid; D002118:Calcium