Probing the structure of 16 S ribosomal RNA from Bacillus brevis.
A majority (approximately 89%) of the nucleotide sequence of Bacillus brevis 16 S rRNA has been determined by a combination of RNA sequencing methods. Several experimental approaches have been used to probe its structure, including (a) partial RNase digestion of 30 S ribosomal subunits, followed by two-dimensional native/denatured gel electrophoresis, in which base-paired fragments were directly identified; (b) identification of positions susceptible to cleavage by RNase A and RNase T1 in 30 S subunits; (c) sites of attack by cobra venom RNase on naked 16 S rRNA; and (d) nucleotides susceptible to attack by bisulfite in 16 S rRNA. These data are discussed with respect to a secondary structure model for B. brevis 16 S rRNA derived by comparative sequence analysis.
D001407:Bacillus / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000032:analysis; D010761:Phosphorus Radioisotopes; D012335:RNA, Ribosomal / Q000302:isolation & purification*; D006163:Ribonuclease T1; D012270:Ribosomes / Q000032:analysis; D013045:Species Specificity
D009843:Oligoribonucleotides; D010761:Phosphorus Radioisotopes; D012335:RNA, Ribosomal; D006163:Ribonuclease T1