Gamma interferon interferes with the negative regulation of macrophage activation by prostaglandin E2.
Activation of mouse macrophages for tumor cell killing is negatively regulated by prostaglandin E2 (PGE2). The effect of this hormone is to shut off cytolytic activity that is expressed as a consequence of activation. A lymphokine in the culture supernates of concanavalin A stimulated spleen cells has been shown to change the sensitivity of activated macrophages to the negative regulatory effects of PGE2, thereby maintaining activation, as manifested by the continued expression of tumor cell killing by these cells. Using a highly specific polyclonal antiserum and gamma interferon produced either by a T-cell hybridoma or by recombinant DNA technology we show here that one lymphokine responsible for mediating the maintenance effect is gamma interferon.
D000818:Animals; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D002478:Cells, Cultured; D002850:Chromatography, Gel; D003602:Cytotoxicity, Immunologic; D015232:Dinoprostone; D006825:Hybridomas / Q000276:immunology; D007371:Interferon-gamma / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009500:Neutralization Tests; D011458:Prostaglandins E / Q000037:antagonists & inhibitors*; D011995:Recombination, Genetic
D000936:Antigen-Antibody Complex; D011458:Prostaglandins E; D007371:Interferon-gamma; D015232:Dinoprostone