[Interpretation of histochemical changes of succinate dehydrogenase activity in liver parenchyma during the monitoring of the clinical course of the acute radiation syndrome: study of sublethally whole-body irradiated rats].
The activity of succinate dehydrogenase (SDH) of liver parenchyma was histochemically determined by use of tetrazolium salts MTT and elutions technique on pubescent male rats prior to irradiation and during 30 days following single total-body X-radiation with 800 R (approx. LD 90/30). These data were compared with those after a single post-radial application of L-cysteine and tetrahydrofolic acid (FH 4). After irradiation and cysteine FH 4 therapy the SDH activity of the liver, in general, showed values which were significantly different from control and experimental groups. The higher enzyme activities were almost exclusively found in the treated animals. The minimum of the regression phase was reached on day 6 after irradiation. Approximately normal values were observed not before two weeks following irradiation, but only as consequence of cysteine/FH 4 therapy. In untreated animals, the SDH activity until on day 30 after irradiation was lower than 50% when compared to normal rats. The determined changes of SDH-activity from day 3 to day 15 after irradiation parallel those post-radial changes of body weight, blood picture and mortality which were found in comparative experiments under identical radiation conditions.
D000818:Animals; D003545:Cysteine / Q000627:therapeutic use; D004338:Drug Combinations; D006651:Histocytochemistry; D008099:Liver / Q000201:enzymology*; D008297:Male; D011833:Radiation Injuries, Experimental / Q000188:drug therapy / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013237:Stereoisomerism; D013385:Succinate Dehydrogenase / Q000378:metabolism*; D013763:Tetrahydrofolates / Q000627:therapeutic use; D014916:Whole-Body Irradiation
D004338:Drug Combinations; D013763:Tetrahydrofolates; C030371:5,6,7,8-tetrahydrofolic acid; D013385:Succinate Dehydrogenase; D003545:Cysteine
Zum Aussagewert histochemischer Veränderungen der Succinatdehydrogenase-Aktivität des Leberparenchyms für die Verlaufsbeobachtung des akuten Strahlensyndroms: Untersuchung an subletal ganzkörperbestrahlten Ratten.