An enzyme-linked immunosorbent assay for the detection of Clostridium perfringens enterotoxin antibody.
An enzyme-linked immunosorbent assay (ELISA) was adapted to test serum antibody to enterotoxin of Clostridium perfringens type A. The test was evaluated using sheep, calf and guinea pig sera and compared with passive hemagglutination and immunodiffusion tests. The ELISA was found to be more sensitive than the other two tests and was completely free from nonspecific reactions. The method was considered to be technically advantageous and suitable for semiautomated procedures.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002417:Cattle; D004768:Enterotoxins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D012756:Sheep
D000907:Antibodies, Bacterial; D004768:Enterotoxins; C035598:enterotoxin, Clostridium