Quantitative extraction of amphotericin B from serum and its determination by high-pressure liquid chromatography.
Therapeutic concentrations of amphotericin B in serum were measured by reversed-phase liquid chromatography with detection at 386 nm. Complete recovery of the drug and the internal standard from a 1-ml serum sample was achieved by pretreating the sample with guanidine hydrochloride and extracting it with a disposable reversed-phase phenyl extraction column. The method was sensitive to 0.005 micrograms of amphotericin B per ml and linear to at least 5 micrograms/ml. Coefficients of variation were 4.8% within-run and 6.3% between-run.
D000666:Amphotericin B / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology; D006801:Humans; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D012995:Solubility
D006146:Guanidines; D012967:Sodium Dodecyl Sulfate; D000666:Amphotericin B; D004492:Edetic Acid; D019791:Guanidine; D004121:Dimethyl Sulfoxide