Formation of additional contacts of chromosome with membrane in the process of DNA repair synthesis in bacterial cells.
An increase in the amount of membrane-bound DNA was found in B. subtilis cells with UV-induced DNA repair synthesis as compared to untreated cells. It was shown that DNA repair synthesis occurred in DNA membrane complexes (DMC) formed during UV-irradiation. UV-induced formation of DMC was observed in cells of wild type strains which were capable of repairing damaged DNA but not in a mutant defective in DNA-polymerase I. It was demonstrated that DNA-polymerase I is located on the membrane of B. subtilis cells. This suggested a participation of DNA-polymerase I in binding of the chromosome to the membrane in UV-irradiated cells. UV-induced DMC did not dissociate when the cells were treated with inhibitors of DNA-gyrase. It, therefore, was qualitatively different from the DMC found during replication. The mechanisms of binding of the damaged DNA to the membrane in UV-irradiated cells of B. subtilis are discussed.
D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002876:Chromosomes, Bacterial / Q000378:metabolism*; D004260:DNA Repair / Q000528:radiation effects; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000528:radiation effects; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D004259:DNA-Directed DNA Polymerase