title: Acrosin activity of canine spermatozoa as an index of cellular damage.
abstract: Eight experiments were performed to validate an extraction technique for canine acrosin and to quantitate the acrosin activity of fresh and frozen-thawed spermatozoa. Acrosin activity from fresh spermatozoa differed amongst dogs and was influenced by the interval since previous ejaculation. Freezing and thawing spermatozoa induced a loss of acrosin activity that differed with the extender in which the spermatozoa were frozen. The assay of acrosin activity, in conjunction with motility estimates, provides a more complete evaluation of the efficacy of seminal extenders in attenuating freezing injury than do motility estimates alone.
mesh_terms: D000176:Acrosin / Q000378:metabolism*; D000818:Animals; D002470:Cell Survival; D004285:Dogs; D010450:Endopeptidases / Q000378:metabolism*; D004792:Enzyme Precursors / Q000378:metabolism; D005615:Freezing; D008297:Male; D012662:Semen Preservation; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology / Q000201:enzymology*
chemical_list: D004792:Enzyme Precursors; D010450:Endopeptidases; C022438:proacrosin; D000176:Acrosin