Detection of blood group A-like substance in bacterial and viral vaccines by countercurrent immunoelectrophoresis using Helix pomatia lectin.
The technique of countercurrent immunoelectrophoresis (CI), using the N-acetyl glucosamine-binding lectin from Helix pomatia, provided a rapid, sensitive, inexpensive, specific and reliable method for assaying blood group A-like substances in both bacterial and viral vaccines. Blood group A-like substance was detected in the pneumococcal polysaccharide vaccine manufactured by Merck Sharp & Dohme up to 1981 and in a staphylococcus vaccine ( Staphage Lysate) manufactured by Delmont Laboratories. Other US licensed vaccines, including diphtheria and tetanus toxoids, pertussis, meningococcal polysaccharide and influenza vaccines, did not contain detectable amounts of this substance. Human anti-A globulins did not provide a satisfactory reagent for the CI assay because they contained precipitating activities to the vaccine components.
D000017:ABO Blood-Group System; D001428:Bacterial Vaccines / Q000032:analysis*; D022121:Cholera Vaccines / Q000032:analysis; D003378:Counterimmunoelectrophoresis; D004168:Diphtheria Toxoid / Q000032:analysis; D006801:Humans; D007252:Influenza Vaccines / Q000032:analysis; D037102:Lectins / Q000032:analysis*; D009345:Neisseria meningitidis / Q000276:immunology; D010567:Pertussis Vaccine / Q000032:analysis; D013296:Streptococcus pneumoniae / Q000276:immunology; D013745:Tetanus Toxoid / Q000032:analysis; D014765:Viral Vaccines / Q000032:analysis*
D000017:ABO Blood-Group System; D001428:Bacterial Vaccines; D022121:Cholera Vaccines; D004168:Diphtheria Toxoid; C038095:Helix lectin; D007252:Influenza Vaccines; D037102:Lectins; D010567:Pertussis Vaccine; D013745:Tetanus Toxoid; D014765:Viral Vaccines