Stimulation by BW755C and inhibition by cysteamine of duodenal epithelial alkaline secretion suggest a role of endogenous prostaglandin in mucosal protection.
Alkaline secretion by 12 mm segments of duodenum just distal to the Brunner's glands area was titrated in situ in anesthetized rats. Intravenous injection of the lipoxygenase inhibitor BW755C (10-20 mg/kg) increased the surface epithelial HCO3- secretion and pretreatment with indomethacin prevented this effect. This supports the view that endogenous production of prostaglandins is important in control of duodenal epithelial HCO3- secretion and ulceroprotection . Pretreatment with cysteamine (100 mg/kg) inhibited the ability of the duodenal surface epithelium to respond to luminal acid with a compensatory rise in alkaline secretion. Measurement of pH at the mucosal surface with microelectrodes revealed that acidification of this surface occurred simultaneously with the decline in alkaline secretion.
D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001639:Bicarbonates / Q000378:metabolism*; D003543:Cysteamine / Q000494:pharmacology*; D004386:Duodenum / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007213:Indomethacin / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011453:Prostaglandins / Q000502:physiology*; D011720:Pyrazoles / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000893:Anti-Inflammatory Agents; D001639:Bicarbonates; D011453:Prostaglandins; D011720:Pyrazoles; D003543:Cysteamine; D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D007213:Indomethacin