Oxytocin: anti-insulin-like effects in isolated fat cells.
Oxytocin has both insulin-like and insulin antagonistic actions in fat cells in vitro. The anti-insulin-like effects of oxytocin in dispersed rat fat cells have been studied. The magnitude of the anti-insulin-like activity varies with the metabolic pathway of glucose utilization; oxidation [14CO2 production], 32%; glycogen synthesis (D-[U-14C] glucose incorporation into glycogen), 77%. In addition, direct inhibition of the activation of fat cell glycogen synthase has been shown. These inhibitory effects depend upon an intact disulfide ring, since the ability of N-ethylmaleimide-reacted oxytocin to inhibit insulin-stimulated processes was reduced by more than 90% when compared to the intact molecule.
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006003:Glycogen / Q000096:biosynthesis; D006006:Glycogen Synthase / Q000037:antagonists & inhibitors; D066298:In Vitro Techniques; D007329:Insulin Antagonists; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D010121:Oxytocin / Q000302:isolation & purification / Q000494:pharmacology*
D007329:Insulin Antagonists; D010121:Oxytocin; D006003:Glycogen; D006006:Glycogen Synthase; D005947:Glucose