Polypeptide induction by recombinant human IFN-gamma.
Partially purified natural human IFN-gamma has been shown to induce the synthesis of a set of unique polypeptides that are not induced by comparable amounts of IFN-alpha and IFN-beta. In addition, a set of polypeptides is induced in common by all three IFNs. The present study utilized a pure recombinant IFN-gamma and a monoclonal antibody against IFN-gamma to investigate whether the unique polypeptide-inducing properties previously reported are due to IFN-gamma itself. With the possible exception of two polypeptides, the results demonstrate conclusively that IFN-gamma is the sole molecular species responsible for the induction of all polypeptides, including those whose induction pattern is unique to IFN-gamma. Very high doses of IFN-alpha and IFN-beta induce some of the latter set of polypeptides to a limited extent. Thus the differential effects of IFN-gamma compared to IFN-alpha and IFN-beta are, in part, quantitative rather than qualitative in nature.
D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D010452:Peptide Biosynthesis
D000911:Antibodies, Monoclonal; D007370:Interferon Type I; D007371:Interferon-gamma