[Production of monoclonal antibodies against gliofibrillary acid protein (GFAP) and their specificity].
Splenic Mice cells immunized with glial fibrillary acidic protein (GFAP) were fused with SP 2/0 myeloma cells. After screening and cloning we obtained two types of hybridomas. Some of them secrete IgG class antibodies, the others IgM class antibodies. The specificity of these antibodies has been tested by three immunoenzymatic methods. The results are that IgG monoclonal antibodies identify an astrocyte-GFAP specific epitope and IgM monoclonal antibodies cross-react with a common epitope to GFAP and vimentin.
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002478:Cells, Cultured; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D007381:Intermediate Filament Proteins / Q000032:analysis*; D051379:Mice; D010954:Plasmacytoma / Q000276:immunology
D000911:Antibodies, Monoclonal; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins
Obtention d'anticorps monoclonaux anti-protéine gliofibrillaire acide (GFAP) et étude de leur spécificité.