Thermal inactivation of mouse brain monoamine oxidase type A and type B.
Mouse brain monoamine oxidase (MAO) type A and type B were incubated at 54 degrees C and samples removed for up to 60 min, and remaining MAO activity was determined. Total MAO activity, type A activity and type B activity all disappeared, presumably due to thermal denaturation, in a time-dependent fashion. The rate of disappearance of MAO type B was faster than that of type A both at pH 7.4 and at pH 9.2, though both types denatured faster at the higher pH compared to the lower pH.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D003010:Clorgyline / Q000494:pharmacology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008995:Monoamine Oxidase / Q000145:classification; D011489:Protein Denaturation; D012642:Selegiline / Q000494:pharmacology
D012642:Selegiline; D008995:Monoamine Oxidase; D003010:Clorgyline