A chemiluminescent probe specific for singlet oxygen.
We have synthesized a methoxyvinylpyrene (MVP) in order to model the mechanism for the observed microsomal chemiluminescence of benzo[a]pyrene 7,8-dihydrodiol, the proximate carcinogenic metabolite of benzo[a]pyrene. This MVP analog has been found to be a highly efficient and specific chemiluminescent probe for picomole quantities of singlet oxygen and singlet oxygen equivalents, and it produces significant chemiluminescence when reacted with cytochrome P-450 enzymes.
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism; D008163:Luminescent Measurements; D008722:Methods; D008862:Microsomes, Liver / Q000378:metabolism; D010100:Oxygen / Q000032:analysis*; D011721:Pyrenes / Q000138:chemical synthesis; D051381:Rats; D026082:Singlet Oxygen
D001580:Benzopyrenes; D011721:Pyrenes; D026082:Singlet Oxygen; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; C043063:(2'-methoxyvinyl)pyrene; D010100:Oxygen