title: Characterization of dermonecrotic toxin produced by serotype D strains of Pasteurella multocida.
abstract: Dermonecrotic toxin (DNT) produced by serotype D strains of Pasteurella multocida, isolated from pigs, was characterized and compared with DNT produced by Bordetella bronchiseptica. The DNT prepared by sonication from P multocida or B bronchiseptica had dermonecrotic activity and lethal toxicity for guinea pigs and mice, and also induced marked atrophy of spleens in the mice. Toxicity of P multocida or B bronchiseptica DNT was completely inactivated by heating at 70 C for 30 minutes, and was reduced by treatment with trypsin, formalin, or glutaraldehyde, indicating that the DNT may be a protein. Although biologic and toxic properties of P multocida DNT were similar to those of B bronchiseptica DNT, cross-neutralization tests between P multocida and B bronchiseptica indicated that DNT from the 2 bacterial species were serologically distinct.
mesh_terms: D000818:Animals; D001884:Bordetella; D003885:Dermotoxins / Q000633:toxicity*; D005557:Formaldehyde / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D006168:Guinea Pigs; D006358:Hot Temperature; D051379:Mice; D009500:Neutralization Tests; D010325:Pasteurella / Q000145:classification; D012703:Serotyping; D013045:Species Specificity; D013552:Swine / Q000382:microbiology; D014357:Trypsin / Q000494:pharmacology
chemical_list: D003885:Dermotoxins; D005557:Formaldehyde; D014357:Trypsin; D005976:Glutaral