von Willebrand Factor/Factor VIII adsorption to surfaces from human plasma.
The adsorption of human von Willebrand factor/factor VIII (VWF/VIII) from citrated plasma to a series of polymeric surfaces and glass was measured with both prelabelled 125I VWF/VIII and an 125I antibody to VWF/VIII. Adsorption to the various surfaces differed but was generally quite low (0.24 to 6.5 ng/cm2). Results from both techniques agreed well. The relationship between VWF/VIII adsorption and the thrombogenicity of the surfaces was examined but no clear trend could be discerned. Further studies will therefore be required to determine whether the variation in VWF/VIII adsorption influences the thrombogenicity of the surfaces.
D000327:Adsorption; D001779:Blood Coagulation Factors / Q000502:physiology*; D005169:Factor VIII / Q000378:metabolism*; D005898:Glass; D006801:Humans; D066298:In Vitro Techniques; D010974:Platelet Aggregation; D011108:Polymers; D013499:Surface Properties; D013927:Thrombosis / Q000209:etiology; D014841:von Willebrand Factor / Q000502:physiology*
D001779:Blood Coagulation Factors; D011108:Polymers; D014841:von Willebrand Factor; D005169:Factor VIII