Kinetic analysis of tetracycline accumulation by Streptococcus faecalis.
Tetracycline transport by Streptococcus faecalis occurs by an energy-dependent, carrier-mediated process. The Michaelis constant for transport was unchanged, but the maximal velocity was increased when an energy source, glucose, was present. Accumulation levels, sometimes 25-fold greater than the external concentration, were controlled by the transport system.
D001693:Biological Transport, Active / Q000187:drug effects; D002751:Chlortetracycline / Q000378:metabolism*; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004734:Energy Metabolism; D013293:Enterococcus faecalis / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D007700:Kinetics; D008911:Minocycline / Q000494:pharmacology; D013752:Tetracycline / Q000378:metabolism*
D004024:Dicyclohexylcarbodiimide; D013752:Tetracycline; D008911:Minocycline; D005947:Glucose; D002751:Chlortetracycline