Defective stimulating capacity of leukocytes in mixed leukocyte culture in constitutional aplastic anemia caused by suppressor T cells. A case study.
A patient with constitutional Fanconi-like aplastic anemia who developed a preleukemic state was transplanted with bone marrow from his HLA-identical MLC-negative brother. Before transplantation his mononuclear leukocytes were found to have a defective stimulating and responder capacity in mixed leukocyte culture with cells of an unrelated individual. After removal of T cells the stimulating capacity was completely restored. MLC testing with separated cell fractions seems important to establish HLA-D identity with a possible donor, especially when MLC tests with unseparated cells are negative.
D000328:Adult; D000741:Anemia, Aplastic / Q000276:immunology*; D016026:Bone Marrow Transplantation; D002469:Cell Separation; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D007962:Leukocytes / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D011289:Preleukemia / Q000628:therapy; D013601:T-Lymphocytes / Q000276:immunology*
D037102:Lectins; D003208:Concanavalin A