Prolonged maintenance of active cytochrome P-450 in adult rat hepatocytes co-cultured with another liver cell type.
The cytochrome P-450 content promptly fell in adult rat hepatocytes cultured in conventional conditions whereas no obvious change was detected over a 10-day period when these cells were co-cultured with another rat liver epithelial cell type. A concomitant maintenance of the amino-pyrine N-demethylase activity and a high aflatoxin B1-induced cytotoxicity was observed, and addition of phenobarbital to the culture medium produced about a 2-fold increase in cytochrome P-450 level. These results indicate that when placed in co-culture, adult hepatocytes remain differentiated for several days and suggest that such a co-culture system is an appropriate model for pharmacotoxicological studies.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000494:pharmacology; D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004847:Epithelial Cells; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D008297:Male; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000348:Aflatoxins; D003470:Culture Media; D003577:Cytochrome P-450 Enzyme System; D016604:Aflatoxin B1; D000633:Aminopyrine N-Demethylase; D010634:Phenobarbital