[BD-40, intercalating 9-azaellipticine derivative, induces breaks in cellular DNA associated with protein bridges].
The nature of the lesions induced in cell DNA by BD-40, a chemical analog of Ellipticine was analyzed by submitting DNA from drug-treated cells to alkaline sucrose gradient centrifugation. DNA size reduction was only observed when cell lysates were digested by proteolytic enzymes. This result indicates that BD-40 induces DNA breaks which are masked by bridges involving DNA-protein interaction. This is a new type of DNA lesion induced in DNA by an intercalating agent.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005347:Fibroblasts; D007211:Indoles / Q000494:pharmacology*; D007364:Intercalating Agents / Q000494:pharmacology*; D007546:Isoquinolines; D051379:Mice; D008807:Mice, Inbred BALB C; D010447:Peptide Hydrolases / Q000494:pharmacology
D004268:DNA-Binding Proteins; D007211:Indoles; D007364:Intercalating Agents; D007546:Isoquinolines; C024256:pazelliptine; D004247:DNA; D010447:Peptide Hydrolases
Le BD-40, intercalant dérivé des aza-9 ellipticines, induit dans l'ADN cellulaire des cassures associées à des pontages protéiques.