[Role of Escherichia coli K-12 lambda phage receptors in transfection and plasmid transformation].
The dependence of competence of Escherichia coli cells in transfection and plasmid transformation from phage lambda receptor protein was studied. Mal+ variants, sensitive to phage lambda (Mal+ lambda S) were obtained from strains with impaired lambda-phage receptor protein (Mal-lambda R). Maximum increase of transfection (4.7-fold) was observed in JC411Mal+ lambda S strain. The efficiency of plasmid transformation of pMB9 and RP4 DNAs was also higher in the strain with functioning lambda-receptor protein.
D010582:Bacteriophage lambda / Q000235:genetics*; D002874:Chromosome Mapping; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D008320:Maltose / Q000378:metabolism; D010957:Plasmids; D011991:Receptors, Virus / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D014170:Transformation, Genetic
D011991:Receptors, Virus; D008320:Maltose
Rol' retseptorov faga lambda u kletok Escherichia coli K-12 v transfektsii i plazmidnoĭ transformatsii.