On the seemingly diminished CO2-Bohr effect in hypoxic chemodenervated rabbits.
In hypoxic rabbits, different levels of Pco2 before and after carotid chemodenervation were applied in order to get information about the acid-base status and the position of the O2-Hb dissociation curve (ODC). A CO2-induced change in pH caused a smaller change in the half-saturation pressure (P50) than was to be expected from the CO2-Bohr effect alone. Considering both, the numerically different CO2- and fixed acid-Bohr factors as well as the corresponding respiratory or metabolic pH changes, a method is presented to calculate the position of the ODC with high accuracy. From these considerations it can be derived that the seemingly diminished CO2-Bohr effect in hypoxic rabbits in vivo, especially after chemodenervation , is due more or less to accumulation of lactid acid. This leads to an increasing error if only the CO2-Bohr factor and the actual pH change are taken into account.
D019794:2,3-Diphosphoglycerate; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004058:Diffusion; D004163:Diphosphoglyceric Acids / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D000860:Hypoxia / Q000378:metabolism*; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D009121:Muscle Denervation; D009132:Muscles / Q000378:metabolism*; D010101:Oxygen Consumption; D010108:Oxyhemoglobins / Q000378:metabolism; D011312:Pressure; D011817:Rabbits
D004163:Diphosphoglyceric Acids; D007773:Lactates; D010108:Oxyhemoglobins; D019794:2,3-Diphosphoglycerate; D002245:Carbon Dioxide; D019344:Lactic Acid