[Comparison of quantity of thyroxine in thyroglobulin and the peptide hormones derived from chromatography and immunoenzyme assay].
An evaluation of the thyroxine content in thyroglobulin and different derived thyroxine-containing peptides by enzyme immunoassay is presented. Results correlate very well with those obtained by ion-exchange chromatography which served as a reference method: r = 0.997, p less than 10(-6). Enzyme immunoassay is more rapid and sensitive than ion-exchange chromatography and then suitable for routine use. Furthermore, this paper shows, for the first time, that enzyme immunoassay can be used to perform direct thyroxine estimation in small peptide (Mr less than 2,500) without previous total enzymatic hydrolysis.
D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D013552:Swine; D013954:Thyroglobulin / Q000032:analysis*; D013963:Thyroid Hormones / Q000032:analysis*; D013974:Thyroxine / Q000032:analysis*
D010446:Peptide Fragments; D010455:Peptides; D013963:Thyroid Hormones; D013954:Thyroglobulin; D013974:Thyroxine
Comparaison du dosage de la thyroxine dans la thyroglobuline et les hormonopeptides dérivés par chromatographie et par immunoenzymologie.