title: Characterization of ATPase in sarcoplasmic reticulum from two strains of dystrophic mice.
abstract: The ATPase activities and phosphoenzyme levels have been determined in sarcoplasmic reticulum (SR) membranes prepared from two animal models of muscular dystrophy, myodystrophic (myd/myd) and strain 129 dystrophic (129 dy/dy) mice. In both myd/myd and 129 dy/dy SR membranes, the basal ATPase activities are elevated above control levels, while the Ca-dependent ATPase activities are normal. The addition of 0.1% Triton X-100 not only lowers the basal ATPase activity of myodystrophic control SR membranes by 60%, but also lowers the elevated basal ATPase activity of myd/myd SR membranes to a similar level. The Ca-dependent ATPase activities of myodystrophic control and myd/myd SR membranes are increased approximately threefold by the addition of Triton. The addition of 0.1% Triton X-100 lowers the basal ATPase activities of 129 control and 129 dy/dy SR membranes to similar levels, but stimulates the CA-dependent ATPase activity of 129 dy/dy SR membranes to a level that is only 60% of that of 129 control SR membranes. The level of phosphoenzyme intermediate is decreased approximately 15% in myd/myd SR membranes and approximately 30% in 129 dy/dy SR membranes.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D006868:Hydrolysis; D007425:Intracellular Membranes / Q000187:drug effects; D051379:Mice; D009137:Muscular Dystrophy, Animal / Q000201:enzymology* / Q000235:genetics*; D017830:Octoxynol; D010750:Phosphoproteins / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*
chemical_list: D010750:Phosphoproteins; D011092:Polyethylene Glycols; D000255:Adenosine Triphosphate; D017830:Octoxynol; D000251:Adenosine Triphosphatases; D002118:Calcium