[A sensitive method for determining the proteolytic activity in culture filtrates].
A new method for the quantitative determination of low proteinase activities in culture filtrates or other complex protein-containing substrates has been developed. The method is based on the inactivation of streptokinase which is monitored by following the reduction of fibrinolysis. In a time interval ranging from 1 to 500 min, the half-life of streptokinase activity is directly proportional to proteinase concentration. The enzymatic reactions are not interfered with by either other proteins or coloured substances.
D000362:Agar; D000818:Animals; D002364:Caseins / Q000378:metabolism; D004058:Diffusion; D010450:Endopeptidases / Q000378:metabolism; D013293:Enterococcus faecalis / Q000201:enzymology*; D006801:Humans; D008892:Milk; D013045:Species Specificity; D013297:Streptococcus pyogenes / Q000201:enzymology*; D013300:Streptokinase / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
D002364:Caseins; D000362:Agar; D010450:Endopeptidases; D013300:Streptokinase; D014357:Trypsin
Ein empfindlicher Nachweis proteolytischer Aktivität in Kulturfiltraten.