Transfection of the DNA polymerase-alpha gene.
DNA polymerase-alpha is the major replicative DNA polymerase in animal cells. The gene coding for a mutant DNA polymerase-alpha was transferred from one cell to another by transfection of DNA from mutant cells. The DNA was isolated from a mutant hamster cell line resistant to aphidicolin, a specific inhibitor of DNA polymerase-alpha, and transferred into an aphidicolin-sensitive cell line. The resulting transfectants exhibited increased survival in the presence of aphidicolin and contained an aphidicolin-resistant DNA polymerase-alpha.
D000818:Animals; D016590:Aphidicolin; D002460:Cell Line; D002999:Clone Cells; D006224:Cricetinae; D003412:Cricetulus / Q000235:genetics; D004257:DNA Polymerase II / Q000235:genetics*; D004224:Diterpenes / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D051379:Mice; D009154:Mutation; D012474:Salmon / Q000235:genetics; D014162:Transfection
D004224:Diterpenes; D016590:Aphidicolin; D004257:DNA Polymerase II