Immunoglobulin light chains in chronic lymphocytic leukemia and related diseases.
The combination of two-dimensional mini gel electrophoresis with the 'western blot' technique proves to be a powerful tool in characterizing lymphoid cells. By testing for kappa and lambda immunoglobulin light chains we were able to differentiate between mono-, oligo-, and polyclonal B-cell diseases. The distribution of the lambda isotypes of 24 cases with chronic lymphocytic B-cell leukemia (B-CLL) tested was not random when compared to the distribution of the lambda light chains in B-lymphocytes of normal persons. This might implicate a genetic link between the lambda loci (chromosome 22) and the development of the lambda-CLL.
D000328:Adult; D000818:Animals; D001402:B-Lymphocytes; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D007147:Immunoglobulin Light Chains / Q000032:analysis*; D007145:Immunoglobulin kappa-Chains / Q000032:analysis; D007146:Immunoglobulin lambda-Chains / Q000032:analysis; D007945:Leukemia, Lymphoid / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D013552:Swine / Q000276:immunology
D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains