Induction of male recombination in Drosophila melanogaster by chemical treatment.
Acridine orange (AO), ethidium bromide (EB), ethyl methanesulfonate (EMS) and 8- ethoxycaffeine ( EOC ) were fed to larvae of Drosophila melanogaster in order to test their capacity for the induction of meiotic recombination in males. Our results show that AO and EB increase significantly the male recombination frequencies. No relationship between chromosome breakage ability and male recombination induction was found since EMS and EOC , two effective chromosome-breaking agents, were unable to increase the male recombination.
D000165:Acridine Orange / Q000494:pharmacology; D000818:Animals; D002110:Caffeine / Q000031:analogs & derivatives / Q000494:pharmacology; D004331:Drosophila melanogaster / Q000235:genetics*; D004996:Ethidium / Q000494:pharmacology; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D008297:Male; D009154:Mutation / Q000187:drug effects*; D011995:Recombination, Genetic / Q000187:drug effects*
C020899:8-ethoxycaffeine; D002110:Caffeine; D005020:Ethyl Methanesulfonate; D004996:Ethidium; D000165:Acridine Orange