The simultaneous identification of seminal acid phosphatase and phosphoglucomutase by starch gel electrophoresis.
Although elevated acid phosphatase (AP) activity in vaginal fluid is a consistent indicator for semen, differentiation between vaginal AP and seminal AP provides a more meaningful result. Detection of seminal AP in mixtures of vaginal AP, feces, and blood is accomplished by starch gel electrophoresis, employing the substrate thymolphthalein monophosphate as a selective visualization agent. Genetic phosphoglucomutase isoenzymes are simultaneously separated by this method and allow differentiation in some semen/vaginal fluid mixtures.
D000135:Acid Phosphatase / Q000032:analysis*; D001826:Body Fluids / Q000201:enzymology; D002021:Buffers; D004592:Electrophoresis, Starch Gel; D005243:Feces / Q000201:enzymology; D005260:Female; D005554:Forensic Medicine; D005782:Gels; D006801:Humans; D008297:Male; D010733:Phosphoglucomutase / Q000032:analysis*; D012007:Rectum / Q000502:physiology; D012661:Semen / Q000201:enzymology*; D014621:Vagina / Q000502:physiology
D002021:Buffers; D005782:Gels; D000135:Acid Phosphatase; D010733:Phosphoglucomutase