Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases.
A mutant strain of Bacillus subtilis carrying lesions in the structural genes for extracellular neutral (nprE) and serine (aprA) proteases was constructed by the gene conversion technique. This mutant had less than 4% of the extracellular protease activity of the wild type and sporulated normally, indicating that neither of these sporulation-associated proteases is essential for development.
D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D002872:Chromosome Deletion; D010450:Endopeptidases / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005838:Genotype; D009154:Mutation; D015260:Neprilysin; D012697:Serine Endopeptidases; D013045:Species Specificity; D013171:Spores, Bacterial / Q000502:physiology
D010450:Endopeptidases; D012697:Serine Endopeptidases; C032923:microbial serine proteinases; D015260:Neprilysin