title: Preferential induction of specific lambda-isotypic antibodies in mice.
abstract: A high proportion (greater than 40%) of lambda-anti-NP antibodies were induced after the administration of hapten conjugates of the relatively T-independent antigen NP-Ficoll. In 11 of 12 strains, lambda 1 anti-NP antibodies were the predominant isotype. In lambda 1-defective SJL mice, lambda 2,3 anti-NP antibodies were the major species after NP-Ficoll immunization. In contrast, the ability to elicit a high proportion of lambda-anti-NP antibodies with the T-dependent conjugate of ovalbumin, NP-OVA, varied among mouse strains. Igh-1b-bearing mice were high producers of lambda 1 anti-NP antibodies (greater than 70% of the response); DBA/2 and BALB/c mice were moderate (40 to 50%) lambda 1 anti-NP producers, and A.TL, AKR, NZB, and C3H mice were low lambda 1 anti-NP producers (less than 10%) after primary NP-OVA immunization. In the latter group, NP-OVA preferentially elicits kappa-bearing anti-NP antibodies. The parameters that influence the distribution of light chain isotypes were investigated. The preferential induction of lambda-anti-NP antibodies with NP-Ficoll was a) partially influenced by Igh-linked genes, b) adjuvant independent, and c) maintained on prolonged immunization. In contrast, induction of a high proportion of kappa-anti-NP antibodies by NP-OVA is (a) strictly regulated by Igh-linked genes and (b) enhanced after hyperimmunization. The immunochemical, genetic, and cellular bases for these observations are discussed.
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis / Q000096:biosynthesis*; D000955:Antigens, T-Independent / Q000008:administration & dosage / Q000276:immunology; D005362:Ficoll / Q000008:administration & dosage / Q000276:immunology; D006168:Guinea Pigs; D007126:Immunoglobulin Allotypes / Q000276:immunology*; D007147:Immunoglobulin Light Chains / Q000276:immunology*; D007146:Immunoglobulin lambda-Chains / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB; D009596:Nitrophenols / Q000008:administration & dosage / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D010648:Phenylacetates
chemical_list: D000276:Adjuvants, Immunologic; D000888:Antibodies, Anti-Idiotypic; D000955:Antigens, T-Independent; D007126:Immunoglobulin Allotypes; D007147:Immunoglobulin Light Chains; D007146:Immunoglobulin lambda-Chains; D009596:Nitrophenols; D010648:Phenylacetates; C026242:4-hydroxy-5-nitrophenyl acetic acid; D005362:Ficoll; D010047:Ovalbumin