[Immunodiagnosis of chronic lymphatic leukemia and leukemic non-Hodgkin's lymphoma with the monoclonal antibodies BL-Ig-L/1, BL-DR/1 and BL-T2].
3 self made monoclonal antibodies are tested with the indirect immunofluorescence method for the immunologic diagnostic of the circulating lymphocytes from 24 chronic lymphatic leukaemia (CLL) and 14 leukaemic Non-Hodgkin lymphoma (NHL) patients. In comparison, investigations were done with various specific rabbit antibody F(ab)2 fragments and antisera. The monoclonal antibody BL-Ig-L/1, which is directed to the human Ig L-chains, marked the neoplastic lymphocytes from 12 of 24 CLL and from 9 of 14 leukaemic NHL as membrane Ig+. The monoclonal antibody BL-DR/1, which reacts with a HLA-DR, binds to the blood lymphocytes of 23 CLL and 11 NHL patients. By this BL-DR/1 is superior to BL-Ig-L/1 for the immunologic diagnostic of the non-T-cell neoplasia. The with normal peripheral T-cells reactive monoclonal antibody BL-T2 reacts with malignant B lymphocytes on an equal scale as BL-DR/1. It is not qualified for the differentiation of malignant blood lymphocytes.
D000911:Antibodies, Monoclonal; D005455:Fluorescent Antibody Technique; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007146:Immunoglobulin lambda-Chains / Q000276:immunology; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D007146:Immunoglobulin lambda-Chains; D011947:Receptors, Antigen, B-Cell
Immundiagnostik der chronischen lymphatischen Leukämie und leukmischen Non-Hodgkin-Lymphome mit den monoklonalen Antikörpern BL-Ig-L/1, BL-DR/1 und BL-T2.