Attempts to characterize the T-cell population and lymphokine involved in the activation of macrophage oxygen metabolism in murine listeriosis.
Cultures of T cells from Listeria monocytogenes-immune mice, macrophages, and heat-killed Listeria organisms produced a factor(s) capable of activating macrophage oxygen metabolism. The activity depended on the presence of Lyt 1+2,3- T cells in the primary culture. Macrophage oxygen metabolism could also be induced by a L. monocytogenes-specific T-cell clone which was recently shown to mediate anti-listerial protection in vivo and to secrete interferon-gamma (IFN-gamma) in vitro. Furthermore, macrophage activation was achieved by recombinant IFN-gamma. It is concluded that acquired resistance to facultative intracellular pathogens--at least in part--depends on the activation of macrophage oxygen metabolism by IFN-gamma derived from specific Lyt 1+2,3- T cells.
D000818:Animals; D002478:Cells, Cultured; D002999:Clone Cells; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000032:analysis; D007518:Isoantibodies / Q000032:analysis; D008088:Listeriosis / Q000276:immunology*; D008163:Luminescent Measurements; D008222:Lymphokines / Q000032:analysis*; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010101:Oxygen Consumption; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D007518:Isoantibodies; D008222:Lymphokines; C029805:Lyt antibodies; D007371:Interferon-gamma