[Immunological specificity and the kinetics of T-suppressor induction in the immunization of mice with allogeneic spleen].
A high dose immunization of mice with gamma-irradiated allogeneic spleen cells has been shown to induce, in a recipient spleen, specific suppressor T-cells, resistant to mitomycin C, which are capable of inhibiting DNA synthesis and, to a lesser degree, the generation of killer cells in the mixed lymphocyte culture (MLC). The maximum suppressor activity is reached on days 3-6 after immunization. Both reactions are blocked mostly in those stimulator cells which bear H-2 antigens used for immunization. In contrast, DNA synthesis is inhibited only slightly, if at all, when it is stimulated in MLC by third-party cells, even if these are added to the culture as a mixture with correspoding stimulators. Unlike X-irradiated allogeneic cells, the untreated ones induce a mixture of suppressors, T-cells and macrophages, with a considerable non-specific suppression. Untreated syngenic lymphoid cells induce less active non-specific suppressors with properties of macrophages.
D000818:Animals; D000918:Antibody Specificity; D004306:Dose-Response Relationship, Immunologic; D007114:Immunization / Q000379:methods*; D007700:Kinetics; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology / Q000637:transplantation*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D013997:Time Factors; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
Immunologicheskaia spetsifichnost' i kinetika induktsii T-supressorov pri immunizatsii mysheĭ allogennoĭ selezenki.