Ionophore A23-187 induces responses to TCGF in mouse lymphocytes.
The process of activation of T lymphocytes by ionophoric substances was analysed according to current concepts of T-lymphocyte activation. Proliferation induced by the ionophore A23-187 was found to be strictly dependent on the exogenous availability of growth factors. Lack of production of growth factors in spleen cell cultures containing the ionophore A23-187 explained the failure to directly stimulate mouse T-lymphocyte proliferation. It was concluded that the main action of A23-187 is the induction of expression of growth receptors, and that the increase in Ca++ uptake is, in itself, not sufficient to induce mitotic events.
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000502:physiology; D003165:Complement System Proteins / Q000276:immunology; D007376:Interleukin-2 / Q000096:biosynthesis*; D007518:Isoantibodies / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011971:Receptors, Immunologic / Q000378:metabolism; D015375:Receptors, Interleukin-2; D013154:Spleen / Q000187:drug effects; D013601:T-Lymphocytes / Q000276:immunology*
D007376:Interleukin-2; D007518:Isoantibodies; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; C035678:anti-Thy antibody; D000001:Calcimycin; D003165:Complement System Proteins; D002118:Calcium