Biological properties of a plaque-inducing agent obtained from Acholeplasma oculi.
A plaque-forming agent arose spontaneously during cloning of Acholeplasma oculi 19L. The agent produced plaques on A. oculi 19L and A. oculi-i, but not on A. laidlawii, A. modicum, or wild isolates of A. oculi. The agent required horse serum for plaque formation as well as for adsorption to the indicator lawn; however, it was extremely sensitive to an inhibitor in some horse sera. The agent retained infectivity after passage through a 50 nm filter and was heat-, Nonidet P-40-, and chloroform-labile, but relatively ether-stable. It was not determined whether the agent is a virus or a bacteriocin-like substance.
D000127:Acholeplasma; D001430:Bacteriocins / Q000502:physiology*; D001435:Bacteriophages / Q000187:drug effects / Q000302:isolation & purification* / Q000502:physiology*; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D014461:Ultracentrifugation; D010948:Viral Plaque Assay
D001430:Bacteriocins; D008937:Mitomycins; D016685:Mitomycin