title: Quantitation of base substitutions in eukaryotic 5S rRNA: selection for the maintenance of RNA secondary structure.
abstract: Eukaryotic 5S rRNA sequences from 34 diverse species were compared by the following method: (1) The sequences were aligned; (2) the positions of substitutions were located by comparison of all possible pairs of sequences; (3) the substitution sites were mapped to an assumed general base pairing model; and (4) the R-Y model of base stacking was used to study stacking pattern relationships in the structure. An analysis of the sequence and structure variability in each region of the molecule is presented. It was found that the degree of base substitution varies over a wide range, from absolute conservation to occurrence of over 90% of the possible observable substitutions. The substitutions are located primarily in stem regions of the 5S rRNA secondary structure. More than 88% of the substitutions in helical regions maintain base pairing. The disruptive substitutions are primarily located at the edges of helical regions, resulting in shortening of the helical regions and lengthening of the adjacent nonpaired regions. Base stacking patterns determined by the R-Y model are mapped onto the general secondary structure. Intrastrand and interstrand stacking could stabilize alternative coaxial structures and limit the conformational flexibility of nonpaired regions. Two short contiguous regions are 100% conserved in all species. This may reflect evolutionary constraints imposed at the DNA level by the requirement for binding of a 5S gene transcription initiation factor during gene expression.
mesh_terms: D000828:Animal Population Groups / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D005658:Fungi / Q000235:genetics; D006801:Humans; D009154:Mutation; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D010944:Plants / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D012984:Software
chemical_list: D012335:RNA, Ribosomal