Vibrio cholerae hemagglutinin/protease nicks cholera enterotoxin.
Unnicked cholera enterotoxin was isolated from culture supernatants of Vibrio cholerae 569B by either rapid processing of flask-grown cultures or by growing and processing fermentor cultures in the presence of ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetra acetic acid, an inhibitor of the previously described V. cholerae hemagglutinin/protease. When unnicked cholera enterotoxin was incubated with purified hemagglutinin/protease, the unnicked A subunit was converted to a molecular weight consistent with that of the A1 subunit as demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and its specific activity for Y1 adrenal cells increased.
D002772:Cholera Toxin / Q000378:metabolism*; D004533:Egtazic Acid / Q000494:pharmacology; D010450:Endopeptidases / Q000378:metabolism*; D008666:Metalloendopeptidases; D013379:Substrate Specificity; D014734:Vibrio cholerae / Q000201:enzymology*
D004533:Egtazic Acid; D002772:Cholera Toxin; D010450:Endopeptidases; D008666:Metalloendopeptidases; C039814:hemagglutinin-protease