Changes of aldolase A and B messenger RNA levels in rat liver during azo-dye-induced hepatocarcinogenesis.
The expression of aldolase A and B mRNAs during azo-dye-induced carcinogenesis in rat liver was examined. After feeding the dye for 18 weeks, the level of aldolase A mRNA increased to about 11 times that in a normal liver, with the concomitant decrease of aldolase B mRNA level to about 25% of that in a normal liver. These changes did not occur progressively during the carcinogenesis, but occurred as an additional phase after 4 week-feeding of the azo-dye. At this stage, the levels of aldolase A and B mRNAs were about 7 times and 45% of that in a normal liver, respectively. This biphasic pattern in the aldolase isozyme expression in the azo-dye-fed rat liver is discussed together with the kinetic data of the enzyme activity.
D000818:Animals; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics* / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000473:pathology; D008297:Male; D008749:Methyldimethylaminoazobenzene / Q000633:toxicity*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic / Q000187:drug effects*; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives*
D007527:Isoenzymes; D012333:RNA, Messenger; D008749:Methyldimethylaminoazobenzene; D004124:p-Dimethylaminoazobenzene; D005634:Fructose-Bisphosphate Aldolase