Degradation of chondroitin 4-sulphate by tri-fluoroacetolysis: isolation of oligosaccharides from the carbohydrate-protein linkage region.
Oligosaccharides from the linkage-region tetrasaccharide, beta-D-GlcpA-(1----3)-beta-D-Galp-(1----3)-beta-D-Galp-(1----4)-D- Xylp, of chondroitin 4-sulphate were isolated after trifluoroacetolysis. The oligosaccharides were purified by ion-exchange chromatography and paper chromatography and subjected to sugar and methylation analysis and g.l.c.-m.s. The recovery of linkage-region oligosaccharides was approximately 45% after trifluoroacetolysis, calculated according to the D-xylose present in the chondroitin 4-sulphate preparation. The following structures were identified: beta-D-Galp-(1----4)-D-Xylp, beta-D-Galp-(1----3)-D-Galp, beta-D-Galp-(1----3)-beta-D-Galp-(1----4)-D-Xylp, beta-D-GlcpA-(1----3)-beta-D-Galp-(1----3)-beta-D-Galp-(1----4)-D- Xylp.
D000818:Animals; D002236:Carbohydrate Conformation; D002417:Cattle; D002807:Chondroitin / Q000031:analogs & derivatives; D002809:Chondroitin Sulfates; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D006023:Glycoproteins; D013058:Mass Spectrometry; D008745:Methylation; D009300:Nasal Septum; D009844:Oligosaccharides / Q000302:isolation & purification*; D014269:Trifluoroacetic Acid
D006023:Glycoproteins; D009844:Oligosaccharides; D002807:Chondroitin; D002809:Chondroitin Sulfates; D014269:Trifluoroacetic Acid