title: Determination of thiamine modification by polyphenols using K3Fe(CN)6, HgCl2 and CNBr.
abstract: The reaction between thiamine (37 micro M) and polyphenols (74 micro M) at 60 degrees, pH 7.4, is without an extremely rapid initial phase. The time-course of reaction is hyperbolic and the log [thiamine] vs time plots pseudo-first order with respect to thiamine. A tentative mechanism is proposed for the reaction after considering results with free radical scavengers and reducing agents together with previous data. As a by-product of the study, CNBr emerges as a better reagent than HgCl2 and K3Fe(CN)6 for thiochrome formation in the presence of polyphenols and reducing agents.
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D003488:Cyanogen Bromide; D005292:Ferricyanides; D005609:Free Radicals; D007700:Kinetics; D008627:Mercuric Chloride; D008628:Mercury; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010636:Phenols; D013831:Thiamine / Q000032:analysis
chemical_list: D005292:Ferricyanides; D005609:Free Radicals; D010636:Phenols; C007931:hexacyanoferrate III; D008627:Mercuric Chloride; D008628:Mercury; D003488:Cyanogen Bromide; D013831:Thiamine