Antibodies specific for the phi 29 terminal protein inhibit the initiation of DNA replication in vitro.
The phi 29 DNA-terminal protein serves as a primer for the initiation of DNA replication by covalently binding the first nucleotide in the DNA chain. Two distinct antibodies were used for functional analysis of this protein. One antibody was raised against sonicated phi 29 DNA-protein complex isolated from phage virions (anti-TP). The other antibody was raised against a conjugate of bovine serum albumin and a synthetic peptide corresponding to the carboxy-terminal of the phi 29 terminal protein (anti-gp3C), which was predicted from the nucleotide sequence of phi 29 DNA. Both antibodies react with native phi 29 terminal protein as determined by immunoprecipitation and enzyme-linked immunosorbent assay. Both antibodies specifically inhibit the complex-forming reaction between the phi 29 terminal protein and dAMP, the first nucleotide of phi 29 DNA.
D000595:Amino Acid Sequence; D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D001412:Bacillus subtilis; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004261:DNA Replication; D003838:Deoxyadenine Nucleotides; D004797:Enzyme-Linked Immunosorbent Assay; D007700:Kinetics; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D003838:Deoxyadenine Nucleotides; D014764:Viral Proteins; C040784:terminal protein, Bacillus phage phi29; C012873:2'-deoxy-5'-adenosine monophosphate