Receptor and non receptor-mediated activation of blood platelets. Effect on membrane-cytoskeleton interaction.
Platelets were activated by receptor and non receptor-mediated reagents. The effect of these reagents on aggregation, secretion, cytoskeleton formation, interaction of alpha-actinin and other membrane proteins with the cytoskeleton was studied. Results show that receptor-mediated activation (e.g. ADP or thrombin activation) leads to a high extent of association of alpha-actinin with the cytoskeleton while non receptor-mediated activation (e.g. ionophore A-23187, arachidonic acid) leads to a low association between the two species. The degrees of aggregation, secretion and total amount of protein in the two modes of activation were the same.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism* / Q000648:ultrastructure; D000001:Calcimycin / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D003488:Cyanogen Bromide; D003599:Cytoskeleton / Q000378:metabolism*; D004586:Electrophoresis / Q000379:methods; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology; D008565:Membrane Proteins / Q000097:blood*; D011485:Protein Binding; D013917:Thrombin / Q000494:pharmacology; D014909:Wheat Germ Agglutinins
D001095:Arachidonic Acids; D037102:Lectins; D008565:Membrane Proteins; D014909:Wheat Germ Agglutinins; D016718:Arachidonic Acid; D000001:Calcimycin; D000244:Adenosine Diphosphate; D013917:Thrombin; D003488:Cyanogen Bromide