[Quantitative method of measuring platelet ATP released in vitro in the presence of various aggregating agents].
A new method for quantitative study of the release of ATP during platelet aggregation is described. The measures have been done by bioluminescence with ADP, collagen, arachidonic acid as inducing agents in 20 normal subjects. Different characteristics of quality of the method have been evaluated to define it as a "routine" test. The results, expressed in moles (M) of ATP by 1 X 10(6) platelets, are different according to the various inducing agents. In association with the study of the platelet aggregation this method should allow to have a better understanding of the anomalies of platelet functions in different diseases, and, may be, to define states of platelet "activation" as done by the study of the release of beta-thromboglobulin and of platelet factor 4.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000097:blood*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism*; D003094:Collagen / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D010974:Platelet Aggregation / Q000187:drug effects*
D001095:Arachidonic Acids; D016718:Arachidonic Acid; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D003094:Collagen
Méthode quantitative de mesure de l'ATP plaquettaire libérée in vitro en présence de différents agents agrégants.