Modifications in the myeloclastogenic effect of benzene in mice with toluene, phenobarbital, 3-methylcholanthrene, Aroclor 1254 and SKF-525A.
Benzene was studied in its target organ of effect, the bone marrow, with the micronucleus test and metaphase analysis. Male and female CD-1 mice were treated with 2 doses of benzene (440 mg/kg) or toluene (860 or 1720 mg/kg) or both 24 h apart, and sacrificed 30 h (or 54 h) after the first dose. Benzene-treated animals were pretreated with phenobarbital (PB), 3-methylcholanthrene (3-MCA), SKF-525A, or Aroclor 1254. Toluene showed no clastogenic activity and reduced the clastogenic effect of benzene when the mixture was given. None of the pretreatments protected against the clastogenic effect of benzene. 3-MCA pretreatment greatly promoted benzene myeloclastogenicity. Females were consistently more resistant to benzene than males. Dose-response curves in benzene-treated mice were much steeper with 3-MCA induction than without. Chromosomal damage was higher with p.o. than i.p. benzene administration.
D000818:Animals; D001140:Aroclors / Q000494:pharmacology*; D001554:Benzene / Q000633:toxicity*; D001853:Bone Marrow / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects*; D020111:Chlorodiphenyl (54% Chlorine); D004347:Drug Interactions; D005260:Female; D008297:Male; D008677:Metaphase / Q000187:drug effects; D008748:Methylcholanthrene / Q000494:pharmacology*; D051379:Mice; D009152:Mutagenicity Tests; D009153:Mutagens; D009154:Mutation; D010634:Phenobarbital / Q000494:pharmacology*; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D011335:Proadifen / Q000494:pharmacology*; D012737:Sex Factors; D014050:Toluene / Q000494:pharmacology*
D001140:Aroclors; D009153:Mutagens; D020111:Chlorodiphenyl (54% Chlorine); D014050:Toluene; D008748:Methylcholanthrene; D011335:Proadifen; D011078:Polychlorinated Biphenyls; D001554:Benzene; D010634:Phenobarbital