[Separation of lymphocytes by using visotrast 370 (author's transl)].
Enrichments of lymphocytes are mainly performed according to the flotation principle. By means of numerous differences with regard to the composition and density of the solution for separation, the relative acelleration and duration of the centrifugation the advantages and disadvantages of at least 7 methods were compared with our own modification: 1. Visotrast 370 diluted with Aqua dest. is equal to density gradients containing ficoll. 2. The one-step assays proved to be better and less time consuming than the combined methods. 3. With the density of the flotation medium of 1,076 g . cm-3 79 +/- 11% of the lymphocyte of the original blood were isolated, their purity was 93 +/- 3%, their vitality 91 +/- 4%. 4. There was slight contamination with other blood cells.
D000100:Acetrizoic Acid; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D007958:Leukocyte Count; D008214:Lymphocytes / Q000276:immunology*
D000100:Acetrizoic Acid
Lymphozytenseparierung unter Verwendung von Visotrast 370.