Elemental composition of bacterial metachromatic inclusions determined by electron microprobe X-ray analysis.
Electron microscopy and microprobe X-ray analysis were used to study metachromatic inclusions of Spirillum itersonii , Corynebacterium diphtheriae, and Micrococcus luteus. In situ metachromatic inclusions were electron dense and contained phosphorus and divalent cations. Metachromatic inclusions isolated by anion-exchange column chromatography and by isoosmolar Metrizamide density gradient centrifugation were similar in composition to in situ inclusions.
D002118:Calcium / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002852:Chromatography, Ion Exchange; D002863:Chromogenic Compounds / Q000032:analysis* / Q000302:isolation & purification; D003353:Corynebacterium diphtheriae / Q000032:analysis*; D003594:Cytoplasmic Granules; D004577:Electron Probe Microanalysis; D008274:Magnesium / Q000032:analysis; D008837:Micrococcus / Q000032:analysis*; D010758:Phosphorus / Q000032:analysis; D011188:Potassium / Q000032:analysis; D011506:Proteins; D013139:Spirillum / Q000032:analysis*
D002863:Chromogenic Compounds; D011506:Proteins; D010758:Phosphorus; D008274:Magnesium; D011188:Potassium; D002118:Calcium