title: Tensile forces enhance prostaglandin E synthesis in osteoblastic cells grown on collagen ribbons.
abstract: An experimental system has been developed to examine the prostaglandin (PG) production induced by tensile mechanical forces in bone cells cultured on collagen ribbons. Fetal rat calvaria cells (osteoblast-enriched) were grown on collagen ribbons. The collagen ribbons were stretched under culture conditions in a machine that recorded force and displacement. Repeated stretching of the collagen ribbons (8 times, 5-10%, over 2 hours) increased the rate of prostaglandin synthesis approximately 3.5-fold over that of cells on nonstretched ribbons. This system should provide a suitable method to quantitatively study the effect of mechanical forces on various parameters of PG synthesis.
mesh_terms: D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen; D003470:Culture Media; D004247:DNA / Q000032:analysis; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D011458:Prostaglandins E / Q000032:analysis / Q000096:biosynthesis*; D011863:Radioimmunoassay; D051381:Rats; D013314:Stress, Mechanical; D013997:Time Factors
chemical_list: D003470:Culture Media; D011458:Prostaglandins E; D003094:Collagen; D004247:DNA