Syngeneic transplantation of cryopreserved fetal mouse proislets.
Proislets, derived from fetal mouse pancreata by collagenase digestion and subsequent organ culture, can be frozen to -196 degrees C and stored in a viable condition before successful syngeneic transplantation. Cryopreserved proislets are relatively undifferentiated morphologically, but continue to differentiate into mature islets after transplantation.
D000818:Animals; D005333:Fetus / Q000378:metabolism; D005615:Freezing; D007515:Islets of Langerhans / Q000196:embryology; D016381:Islets of Langerhans Transplantation; D051379:Mice; D008808:Mice, Inbred CBA; D011309:Preservation, Biological; D051381:Rats