title: Fanconi anaemia cells are not uniformly deficient in unhooking of DNA interstrand crosslinks, induced by mitomycin C or 8-methoxypsoralen plus UVA.
abstract: Fanconi anaemia (FA) cells are extremely sensitive to crosslinking agents, e.g. mitomycin C, but only moderately sensitive to trimethylpsoralen plus UVA. Evidence has been reported suggesting that there is a deficient DNA crosslink repair mechanism in FA cells, but others failed to confirm this conclusion using other methods and other crosslinking agents. We reinvestigated the mitomycin C and 8-methoxypsoralen crosslink repair in FA cells with a high sensitivity to mitomycin C. Although an essentially similar methodology was used to that previously described, no difference between the control and FA cell strains was observed, neither for mitomycin C- nor for 8-methoxypsoralen-induced crosslinks.
mesh_terms: D002460:Cell Line; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004260:DNA Repair; D005199:Fanconi Anemia / Q000235:genetics; D011564:Furocoumarins / Q000494:pharmacology*; D006801:Humans; D008730:Methoxsalen / Q000494:pharmacology*; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology*; D010641:Phenotype; D014307:Trioxsalen / Q000494:pharmacology*
chemical_list: D003432:Cross-Linking Reagents; D011564:Furocoumarins; D008937:Mitomycins; D016685:Mitomycin; D008730:Methoxsalen; D014307:Trioxsalen