Human T lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies. A comparison.
Human T cell subpopulations have been defined on the basis of differential expression of either Fc receptors or specific cell-surface antigens. In this study, we utilized a series of monoclonal antibodies reactive with T cells, monocytes, and Ia antigens to characterize isolated subpopulations of T cells bearing receptors for the Fc portion of IgG (T gamma) and subpopulations of T cells bearing receptors for the Fc portion of IgM T mu. The results showed that the T mu population contained both inducer (OKT4+) and cytotoxic/suppressor (OKT5+) populations and was similar to the unfractionated T cell population, whereas the T gamma subset contained few T lymphocytes (OKT3+) and was not enriched for either T cell subset defined by these monoclonal antibodies. Rather, the T gamma population was comprised largely of Ia- cells possessing a monocyte antigen (OKM1+). In reciprocal studies, it was found that both isolated OKT4+ and OKT5+ T cell subsets contained few T gamma cells, whereas both subsets were mainly comprised of T mu cells. We conclude that there is little correlation between T cell subsets defined by these monoclonal antibodies and those defined by Fc receptors.
D000918:Antibody Specificity; D000954:Antigens, Surface / Q000032:analysis*; D002469:Cell Separation / Q000379:methods; D002999:Clone Cells / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008211:Lymphocyte Cooperation; D011961:Receptors, Fc / Q000032:analysis*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000954:Antigens, Surface; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011961:Receptors, Fc