Measurement of factor VIIIRAg-1. A method for use in the Hyland laser nephelometer.
Factor VIIIRAg was measured by laser nephelometry using a new diluent containing a citrate-carbonate buffer, pluronic polyol P94 and triton X100. No pre-treatment of the samples was required, the incubation time was 0.5 to 1.5 hours depending on the antiserum used, and the tests could be read during the following 4 hours (plasma) or 1.5 hours (intermediate purity F VIII). The CV for the assay was less than 10% for plasma or dilutions of plasma in which the minimum detectable amount of factor VIIIRAg was about 0.04 u/ml. A significant correlation was obtained between the nephelometric method and the Laurell rocket method using plasma, but agreement between the methods when therapeutic products were assayed was poor.
D000941:Antigens / Q000032:analysis*; D005169:Factor VIII / Q000032:analysis / Q000276:immunology*; D006801:Humans; D007122:Immunoelectrophoresis; D007834:Lasers; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation; D013048:Specimen Handling; D014841:von Willebrand Factor
D000941:Antigens; D014841:von Willebrand Factor; D005169:Factor VIII