Inhibition of neutrophil chemotaxis by organotin compounds.
Organotin compounds such as Bu2SnCl2, Bu3SnCl and Ph3SnCl suppressed significantly not only chemotactic response of neutrophils to stimulation by the chemoattractant fMet-Leu-Phe but also phospholipase activity in situ as measured by the release of [1-14C] arachidonic acid previously incorporated into phospholipids. Moreover, these suppressions were dose dependent and a parallelism was found between dose-dependent inhibition of chemotaxis and that of arachidonate release. These results suggest that the chemotactic response is controlled by the activation of phospholipase activity in neutrophils, and that the inhibitory effects of these organotin compounds on chemotactic response reflect the blockage of phospholipase activation system regulated by phosphorylation of lipomodulin.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils; D009947:Organotin Compounds / Q000494:pharmacology*; D011817:Rabbits; D013997:Time Factors; D014220:Trialkyltin Compounds / Q000494:pharmacology
D001095:Arachidonic Acids; D009947:Organotin Compounds; D014220:Trialkyltin Compounds; D016718:Arachidonic Acid; C011559:tributyltin; D009240:N-Formylmethionine Leucyl-Phenylalanine; C030665:triphenyltin; C020735:dibutyldichlorotin