[Determination of the chemotaxis of peritoneal macrophages from mice by means of different test systems (author's transl)].
The chemotactical response of mouse peritoneal macrophages was examined by means of three different methods: 1. A modified migration chamber technique 2. An agarose assay 3. A 51chromium labelling assay. Casein and the synthetical peptide N-formylmethionin leucine were applied as chemoattractants. The results show that the migration chamber technique is more sensitive and therefore more suitable to determine the chemotactical response of mouse peritoneal macrophages than the assay under agarose. An additional labeling of macrophages with 51chromium in the migration chamber technique is inefficient.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002464:Cell Migration Inhibition; D002860:Chromium Radioisotopes; D005260:Female; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains
D002860:Chromium Radioisotopes
Bestimmung der Chemotaxis von Peritonealmakrophagen der Maus mit verschiedenen Testsystemen.