title: Energetics of the one-electron steps in the NAD+/NADH redox couple.
abstract: The one-electron reduction potential of NAD+ has been determined using pulse radiolysis to study electron-transfer equilibria between it and a low potential bipyridylium compound. The determined value E1 electron pH7 (NAD+/NAD) = -922 +/- 8 mV (NHE scale) is used to calculate E2 electrons pH7 (NAD/NADH) =  +282 mV. E1 electron pH7 for 1-methylnicotinamide, E1 electron pH7 (MeN+/MeN) = -918 +/- 7 mV.
mesh_terms: D004583:Electrons; D007700:Kinetics; D009243:NAD; D009536:Niacinamide / Q000031:analogs & derivatives; D010084:Oxidation-Reduction; D011199:Potentiometry
chemical_list: D009243:NAD; D009536:Niacinamide; C024058:N(1)-methylnicotinamide