[Reaction between isolated lambda phage DNA and membrane structures of Escherichia coli cells].
The saccharose density gradient (30--55%) centrifugation technique applied to E. coli membrane preparations was used to show that treatment of the bacteria with Ca2+ in the cold results in the redistribution of the absorbed phage DNA from the cell wall to the cytoplasmic membrane while freezing-thawing of the bacteria leads to equal distribution of the infectious DNA among all membrane fractions. Quantitative estimation of such a redistribution is reported.
D010582:Bacteriophage lambda / Q000235:genetics*; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane; D002473:Cell Wall / Q000378:metabolism; D003080:Cold Temperature; D004279:DNA, Viral / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D005615:Freezing; D013347:Subcellular Fractions / Q000378:metabolism
D004279:DNA, Viral; D002118:Calcium
Vzaimodeĭstvie izolirovannoĭ DNK faga lambda s membrannymi strukturami kletok Escherichia coli.