A new method for measuring respiration in the conscious mouse.
A highly sensitive apparatus and method for measuring respiratory changes in conscious mice and rats is described. The apparatus is constructed from readily available components and consists of three sections: 1) mouse housing capsule and respiratory-sensitive part; 2) air supplying part; and 3) data recording part. Changes in the respiration of an encapsuled mouse can be accurately detected by means of changes in pressure exerted on the respiratory-sensitive part attached to the capsule. In our study, the rapid increases and decreases in the respiratory rates of these animals after the i.v. administration of respiratory depressing agents (morphine and arachidonate) and a respiratory stimulant (dimorpholamine) were recorded with exceptional accuracy by this procedure.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D051379:Mice; D009020:Morphine / Q000494:pharmacology; D009025:Morpholines / Q000494:pharmacology; D051381:Rats; D012119:Respiration / Q000187:drug effects*; D012129:Respiratory Function Tests / Q000295:instrumentation*
D001095:Arachidonic Acids; D009025:Morpholines; D016718:Arachidonic Acid; D009020:Morphine; C100074:dimorpholamine