title: Proteins shifting from the cytoplasm into the nuclei during early embryogenesis of Drosophila melanogaster.
abstract: Monoclonal antibodies have been used to study the distribution of several proteins in cleavage and blastoderm stages of Drosophila melanogaster. These antigens are known to be associated with hnRNA-containing particles in tissue culture cells. Protein blotting shows that they are present in the embryo 1 hr after egg deposition. A redistribution from the cytoplasm into the somatic nuclei can be observed during developmental stage 12/13, one stage prior to the formation of the cellular blastoderm. Yolk nuclei become stained by these antibodies at about the same time. The shift into pole cell nuclei, however, occurs 1 1/2 hr later, during the migration of these cells into the posterior midgut rudiment.
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex; D000941:Antigens / Q000032:analysis; D001756:Blastoderm / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology*; D003593:Cytoplasm / Q000502:physiology; D004331:Drosophila melanogaster / Q000196:embryology*; D005260:Female; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007399:Interphase; D011506:Proteins / Q000378:metabolism*
chemical_list: D000936:Antigen-Antibody Complex; D000941:Antigens; D007106:Immune Sera; D011506:Proteins