[In vitro activation by dithiothreitol and thioredoxins of carbamyl phosphate synthetase-I in rat liver].
Carbamyl phosphate synthetase-I (CPS-I; EC 6.3.4.16), a mitochondrial enzyme of the urea-cycle, was studied in deactivated extracts of rat liver. It has been found to be activated in vitro by dithiothreitol (DTT) and Mg2+ ions. After reduction by DTT, thioredoxins, isolated from rat liver, were able to activate CPS-I by 468%.
D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology*; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000378:metabolism*; D004229:Dithiothreitol / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D008025:Ligases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013879:Thioredoxins / Q000494:pharmacology*
D001426:Bacterial Proteins; D013879:Thioredoxins; D008025:Ligases; D002222:Carbamoyl-Phosphate Synthase (Ammonia); D008274:Magnesium; D004229:Dithiothreitol
Activation in vitro par le dithiothreitol et les thiorédoxines de la carbamyl phosphate synthétase-I dans le foie de rat.