Cytophotometrical histochemical method of simultaneous measurement of two ingredients (DNA, proteins, enzymes) in the same cell.
There are 2 alternative procedures for cytophotometrical determination of 2 histochemical ingredients (DNA, proteins, and enzymes) in the same cell. The 1st possibility consists of the sequential measurement of 2 ingredients, when the 2nd chromophore is revealed after the determination and extraction of the 1st chromophore. Another one is the simultaneous measurement, when the 2 histochemical reactions (at 1st with the 1st chromophore and then with the other one) are performed on the fixed sections. So each of the sections includes 2 chromophores simultaneously. Next after photometry in 2 wavelengths, the quantity of each chromophore is calculated. This method gives a characteristic of mutual distribution of 2 ingredients in one and the same cell by coefficient of correlation. This article accentuates the causes of the limitations, and the estimation of mistakes. In the appendix there are described methodics and programmes of calculations.
D000110:Acetylcholinesterase / Q000032:analysis; D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D004247:DNA / Q000032:analysis*; D004798:Enzymes / Q000032:analysis; D005724:Ganglia / Q000032:analysis; D005727:Ganglia, Spinal / Q000032:analysis*; D006651:Histocytochemistry / Q000379:methods*; D009474:Neurons / Q000032:analysis*; D010088:Oxidoreductases / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D051381:Rats; D013053:Spectrophotometry / Q000379:methods
D004798:Enzymes; D011506:Proteins; D004247:DNA; D010088:Oxidoreductases; D000110:Acetylcholinesterase