Regulation of ethanolamine and choline phosphatide biosynthesis in isolated rat intestinal villus cells.
The effects of ethanolamine, choline, and different fatty acids on phospholipid synthesis via the CDP-ester pathways were studied in isolated rat intestinal villus cells. The incorporation of [14C]glucose into phosphatidylethanolamine was stimulated severalfold by the addition of ethanolamine and long-chained unsaturated fatty acids, while the addition of lauric acid inhibited the incorporation of radioactivity into phosphatidylethanolamine. At concentrations of ethanolamine higher than 0.2 mM, phosphoethanolamine accumulated, but the concentrations of CDP-ethanolamine and the incorporation of radioactivity into phospatidylethanolamine did not increase further. The incorporation of [14C]glucose into phosphatidylcholine responded in a way similar to that of phosphatidylethanolamine, except that a 10-fold higher concentration of choline was required for maximal stimulation. CCC inhibited the incorporation of choline into phosphatidylcholine. In contrast with hepatocytes, villus cells did not form phosphatidylcholine via phospholipid N-methylation. The data indicate that, in intestinal villus cells, the cytidylyltransferase reactions are rate limiting in the synthesis of phosphatidylethanolamine and probably also of phosphatidylcholine. The availability of diacylglycerol and its fatty acid composition may also significantly affect the rate of phospholipid synthesis.
D000818:Animals; D002716:Chlormequat / Q000494:pharmacology; D002794:Choline / Q000494:pharmacology; D003565:Cytidine Diphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D003566:Cytidine Diphosphate Choline / Q000378:metabolism; D004281:Docosahexaenoic Acids; D004983:Ethanolamines / Q000378:metabolism / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D007850:Lauric Acids / Q000494:pharmacology; D008297:Male; D008745:Methylation; D008861:Microsomes / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D009829:Oleic Acids / Q000494:pharmacology; D010169:Palmitic Acids / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D010714:Phosphatidylethanolamines / Q000096:biosynthesis*; D051381:Rats
D004983:Ethanolamines; D005227:Fatty Acids; D007850:Lauric Acids; D009829:Oleic Acids; D010169:Palmitic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D004281:Docosahexaenoic Acids; C006933:CDP ethanolamine; D003566:Cytidine Diphosphate Choline; D003565:Cytidine Diphosphate; D002716:Chlormequat; D005947:Glucose; D002794:Choline