[Insulin receptors in dog lymphocytes].
The circulating lymphocyte because of its easy accessibility provides a useful model for the study of insulin receptors in dog. Consequently, we have studied the binding of insulin to lymphocytes prepared from Ficoll gradient. Our results demonstrate that circulating dog lymphocytes bind 125I-Insulin and this binding is inhibited by unlabeled insulin. This binding is function of the cell concentration and a saturable function of 125I-Insulin concentration. At 4 degrees C the equilibrium conditions are reached in 30 mn. Scatchard analysis revealed a cirvilinear plot. The number of receptors per cell was calculated as 1000 to 1500. This study have also revealated a decrease in the number of sites in diabetic dogs. We believe that the study of insulin receptor in lymphocyte's dog is important to understand some aspects of pathological states in diabetes.
D000818:Animals; D001667:Binding, Competitive; D004285:Dogs; D007328:Insulin / Q000097:blood; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism*; D011972:Receptor, Insulin / Q000378:metabolism*
D007328:Insulin; D011972:Receptor, Insulin
Récepteurs de l'insuline au niveau des lymphocytes chez le chien.