title: Two-dimensional electrophoresis of human plasma apolipoproteins.
abstract: The two-dimensional electrophoretic method with silver staining we describe better resolves plasma apolipoproteins (apo) than any procedure previously described. It can be used to screen for abnormalities in apoA-I, apoA-II, apoA-IV, apoC-II, apoC-III, apoD, apoE, and apoH. In addition, this is the first presentation of apoD and apoH on two-dimensional gels. This electrophoretic method will permit the quantification of plasma apolipoproteins by computer imaging. Such detailed analysis of the plasma apolipoproteins should provide a better understanding of apolipoprotein function and the role these apolipoproteins play in lipoprotein metabolism and the pathophysiology of dyslipoproteinemias and coronary artery disease.
mesh_terms: D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D053304:Apolipoprotein C-II; D053305:Apolipoprotein C-III; D001053:Apolipoproteins / Q000097:blood* / Q000172:deficiency / Q000235:genetics; D001054:Apolipoproteins A / Q000097:blood; D001055:Apolipoproteins B / Q000097:blood; D001056:Apolipoproteins C / Q000097:blood; D053399:Apolipoproteins D; D001057:Apolipoproteins E / Q000097:blood; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D006023:Glycoproteins / Q000097:blood; D006801:Humans; D007525:Isoelectric Focusing; D010641:Phenotype; D053482:beta 2-Glycoprotein I
chemical_list: D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D053304:Apolipoprotein C-II; D053305:Apolipoprotein C-III; D001053:Apolipoproteins; D001054:Apolipoproteins A; D001055:Apolipoproteins B; D001056:Apolipoproteins C; D053399:Apolipoproteins D; D001057:Apolipoproteins E; D006023:Glycoproteins; C022205:apolipoprotein A-IV; D053482:beta 2-Glycoprotein I