Effects of FSH on acidic nuclear protein synthesis in cultured pig Sertoli cells.
We studied the effect of FHS on nuclear protein synthesis by a primary culture of immature porcine Sertoli cells. The cells were cultured in a chemically defined medium and treated with FSH (50 ng/ml) for various periods (4, 8, 25 and 64 h). About 65 spots (pHi less than 8) were identified by two-dimensional polyacrylamide gel electrophoresis of the radiolabelled nuclear proteins. After 25 h of FSH treatment, we observed an increase of 5 proteins, a slight decrease of two and a disappearance of one. For the short periods of FSH treatment (4 and 8 h), no effect of FHS on nuclear protein synthesis was observed. After 64 h of FSH treatment (4 and 8 h), no effect of FHS on nuclear protein synthesis was observed. After 64 h of FSH treatment, the synthesis of all nuclear proteins appears to be decreased.
D000818:Animals; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D009698:Nucleoproteins / Q000096:biosynthesis* / Q000302:isolation & purification; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism*; D013552:Swine
D009698:Nucleoproteins; D005640:Follicle Stimulating Hormone