title: Suppression of IgA synthesis in chickens.
abstract: Four different protocols were tested for the induction of IgA deficiency in chickens: (I) inoculation of anti-alpha intra-peritoneally (i.p.) on alternate days after hatching up to a period of three weeks; (II) bursectomy within 6 h and at 24 h after hatching; (III) in-ovo injection of anti-alpha on the 17th day of embryonation followed by bursectomy at 24 h of hatch and a single injection of anti-alpha i.p. on the day of hatching; (IV) as in III above but bursectomy within 6 h of hatch, followed by three further injections of anti-alpha on days 3, 10 and 34 after hatching. Treatment (I) produced temporary dysgammaglobulinemia during the period of treatment. Bursectomy at 24 h of hatch rendered 75% of the chicks IgA deficient up to four weeks of age. Early bursectomy within 6 h of hatch resulted in substantial improvement of IgA suppression. Such chicks, when tested at 4, 6 and 10 weeks of age, were found to be 81, 72 and 58.3% IgA deficient, respectively. With treatment (III) all the treated birds were IgA deficient at four weeks of age. However, as the birds grew older, IgA appeared in the serum so that at the age of 12 weeks only 27.3% were deficient. Treatment (IV) completely suppressed the IgA system of 13 out of 14 chickens. These chickens lacked both serum and secretory IgA as well as IgA-containing cells in their intestinal mucosa. Both IgG and IgM continued to be produced.
mesh_terms: D000367:Age Factors; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000008:administration & dosage; D002060:Bursa of Fabricius / Q000276:immunology; D002642:Chick Embryo; D002645:Chickens; D004195:Disease Models, Animal; D004406:Dysgammaglobulinemia / Q000209:etiology*; D017098:IgA Deficiency; D007070:Immunoglobulin A / Q000096:biosynthesis; D007137:Immunoglobulin alpha-Chains / Q000276:immunology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007070:Immunoglobulin A; D007137:Immunoglobulin alpha-Chains