title: Proportion of active dephosphorylated pyruvate dehydrogenase complex in heart and isolated heart mitochondria is decreased in obese hyperinsulinaemic mice.
abstract: The proportion of active, dephosphorylated, pyruvate dehydrogenase complex was decreased in the mouse heart by obesity (by 56%), and this decrease in enzyme activity persisted during preparation and extraction of heart mitochondria. Phosphorylation and inactivation of pyruvate dehydrogenase may be a major factor in mediating the inhibitory effects of obesity on glucose oxidation in muscle, and this may represent an important mechanism in the development and/or expression of cellular insulin-resistance.
mesh_terms: D000818:Animals; D006051:Aurothioglucose; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008929:Mitochondria, Heart / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D009765:Obesity / Q000139:chemically induced / Q000201:enzymology*; D010766:Phosphorylation; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*; D013217:Starvation / Q000201:enzymology
chemical_list: D001786:Blood Glucose; D007328:Insulin; D011768:Pyruvate Dehydrogenase Complex; D006051:Aurothioglucose