title: Synthesis and degradation of aflatoxins by Aspergillus parasiticus. I. Synthesis of aflatoxin B1 by young mycelium and its subsequent degradation in aging mycelium.
abstract: Aflatoxin production and degradation were examined in three isolates of A. parasiticus. Maximum yields were present after incubation for 14 days and these declined gradually as the culture aged. Young mycelia (4 days old) synthesized the greatest amounts of aflatoxin, but aging mycelia (14 days old) were mainly responsible for degradation. Addition of cycloheximide to young cultures and removal of mycelia from aging cultures both prevented further aflatoxin degradation. Intramycelial substances released from fragmented or homogenized mycelium were capable of degrading aflatoxins, and their concentration increased as the mycelium aged. When 14C-labelled aflatoxin was added to a 2-day-old culture and further incubated, 75% of the radioactivity at 12 days was intramycelial, but at 20 days, most radioactivity was in the filtrate.
mesh_terms: D016604:Aflatoxin B1; D000348:Aflatoxins / Q000096:biosynthesis* / Q000378:metabolism*; D001230:Aspergillus / Q000254:growth & development / Q000378:metabolism*; D001711:Biotransformation; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000348:Aflatoxins; D003470:Culture Media; D003513:Cycloheximide; D016604:Aflatoxin B1