Electrophoretic mobility of mouse immune (gamma) interferon produced by a cloned T-cell lymphoma.
In the present study we have analyzed the electrophoretic mobility of murine IFN-gamma produced by a T-cell lymphoma upon stimulation with phorbol myristic acetate. Two peaks of antiviral activity were recovered after gel electrophoresis of partially purified MuIFN-gamma, in the regions of 30 kd and 43 kd. Since MuIFN-gamma displays a molecular weight of about 16-17 kd in denaturating conditions, the above values represent oligomeric forms, probably composed of two or three units of lower molecular weight.
D000818:Animals; D002460:Cell Line; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D007371:Interferon-gamma / Q000235:genetics; D008223:Lymphoma; D051379:Mice; D008970:Molecular Weight; D013601:T-Lymphocytes
D007371:Interferon-gamma