Comparison of the glucosyltransferases of Streptococcus mutans gs-5 to a non-cariogenic mutant.
The extracellular glucosyltransferase (GTF) enzymes of Streptococcus mutans GS-5 and avirulent mutant GS-511 were fractionated using agarose and DEAE-cellulose columns. GTF of GS-5 produced both water-soluble and -insoluble glucans, while those of GS-511 made soluble products almost exclusively. Nearly all (98%) of the small amount of insoluble GTF enzyme made by GS-511 was bound to the cell wall.
D002241:Carbohydrates / Q000032:analysis; D002250:Carbon Radioisotopes; D002473:Cell Wall / Q000201:enzymology; D002847:Chromatography, Agarose; D002848:Chromatography, DEAE-Cellulose; D005964:Glucosyltransferases / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D013295:Streptococcus mutans / Q000201:enzymology* / Q000235:genetics
D002241:Carbohydrates; D002250:Carbon Radioisotopes; D011135:Polysaccharides, Bacterial; D005964:Glucosyltransferases