[Mitogenic factors for BALB/c 3T3 cells isolated from the serum of horses by affinity chromatography on a column using fetal calf serum as the ligand].
Fetal calf serum (SVF), insolubilized on a sepharose matrix, retains mitogenic factors (FM) from horse serum (SC) which can be eluted by molar acetic acid. The FM can: 1 degree initiate the synthesis of DNA in serum starved, contact inhibited 3T3 BALB/c fibroblasts and 2 degrees allow at least three generations to take place. The FM have no antitryptic activity and do not allow cell attachment. Weight to weight, this fraction is 10 to 50 times more active than horse serum. Polyacrylamide gel electrophoresis shows that various proteins are present, particularly immunoglobulins. The intervention of epidermal growth factor (EGF), fibroblast growth factor (FGF) or the platelet-derived growth factor (PDGF) have been excluded.
D000818:Animals; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D002846:Chromatography, Affinity; D005312:Fetal Blood / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology; D006736:Horses / Q000097:blood*; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens / Q000097:blood* / Q000302:isolation & purification
D008934:Mitogens
Des facteurs mitogènes pour des cellules BALB/c 3T3 isolés à partir de sérum de cheval par chromatographie d'affinité sur colonne ayant pour ligand du sérum de veau foetal.