Metabolic activation of procarcinogens to genotoxic products in cultured rat liver cells.
A recently established rat liver cell line, RL-12, exhibits an unusually high sensitivity to the genotoxic effects of a number of selected procarcinogens. Significant reductions in cell survival (D37%) and induction of sister chromatid exchanges were obtained with 1 X 10(-9) M benzo[a]pyrene and 2 X 10(-8) M 7,12-dimethylbenz[a]anthracene. This rat liver epithelial cell line may serve as a useful model system to study the metabolic activation of procarcinogens to their ultimate genotoxic form.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D016604:Aflatoxin B1; D000348:Aflatoxins / Q000633:toxicity; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D001711:Biotransformation; D002273:Carcinogens / Q000378:metabolism / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D003520:Cyclophosphamide / Q000633:toxicity; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D051381:Rats; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D000348:Aflatoxins; D002273:Carcinogens; D001564:Benzo(a)pyrene; D015127:9,10-Dimethyl-1,2-benzanthracene; D003520:Cyclophosphamide; D016604:Aflatoxin B1