An endogenous inhibitor of erythrocyte membrane (Ca2+ + Mg2+)-ATPase involved in calcium transport.
1. An inhibitor of erythrocyte membrane (Ca2+ + Mg+)-ATPase was isolated from membrane-free pig haemolysate through treatment with diethylaminoethyl-Sephacel and carboxymethyl-Sephadex at pH 6.8. The resulting inhibitor preparation was devoid of (Ca2+ + Mg2+)-ATPase activator protein. 2. The inhibitor was without effect on erythrocyte membrane acetylcholinesterase, ouabain-sensitive ATPase and spectrin (Ca2+ + Mg2+)-ATPase. 3. The inhibitor was active in suppressing activator sensitive, ATP-dependent calcium transport across erythrocyte membrane.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors* / Q000097:blood; D002800:Cholinesterase Inhibitors; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000097:blood*; D004910:Erythrocyte Membrane / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D008274:Magnesium / Q000494:pharmacology; D013552:Swine
D002800:Cholinesterase Inhibitors; D004791:Enzyme Inhibitors; D000252:Calcium-Transporting ATPases; D008274:Magnesium; D002118:Calcium