Effect of Brij 58 on the hydrolysis of methyl butyrate by lipase from Pseudomonas fluorescens.
Purified Pseudomonas fluorescens lipase [EC 3.1.1.3] exhibited slight activity on water-soluble esters such as methyl butyrate, and this activity was increased on addition of Brij 58 (20 oxyethylene hexadecyl ether) to the solution. This stimulating effect of Brij 58 on hydrolysis of various esters (dimethyl succinate, butyl n-acetate, and tributyrin) in aqueous solution was unspecific. Hydrolysis of methyl butyrate depended on the molecular ratio of Brij 58 to lipase, being maximal (about 8 times the basal level at 37 degrees C with 80 mM substrate in 0.1 M NaCl solution) with 30 mol of Brij 58 per mol of lipase. Comparative studies showed that all polyoxyethylene (POE) alkyl ethers tested, stimulated the methyl butyrate hydrolyzing activity and that the Adekatol SO series (dihydric normal alcohol ethoxylate) also stimulated the appreciably active, whereas Triton X-100, sodium cholate, sodium deoxycholate, sodium dodecylsulfate, POE, and fatty acids had no effect. Comparison of the effects of Brij 58 on the methyl butyrate hydrolyzing activities of various lipolytic enzymes indicated that its effect was specific for this lipase. Brij 58 had no detectable effect with emulsified esters, such as supersaturated methyl butyrate and triolein.
D000818:Animals; D002087:Butyrates / Q000378:metabolism*; D002175:Candida / Q000201:enzymology; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002417:Cattle; D002592:Cetomacrogol / Q000494:pharmacology*; D003902:Detergents / Q000494:pharmacology; D005260:Female; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D008071:Lipoprotein Lipase / Q000378:metabolism; D008892:Milk / Q000201:enzymology; D009089:Mucor / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011551:Pseudomonas fluorescens / Q000201:enzymology*; D013045:Species Specificity; D013329:Structure-Activity Relationship; D013552:Swine
D002087:Butyrates; D003902:Detergents; D011092:Polyethylene Glycols; D002592:Cetomacrogol; C043811:methyl butyrate; D002265:Carboxylic Ester Hydrolases; D008049:Lipase; D008071:Lipoprotein Lipase