Aminopeptidase in human seminal plasma.
Aminopeptidase of seminal plasma was studied in normozoospermic, oligozoospermic, obstructive and non-obstructive azoospermic men. Significantly higher activity was observed in non-obstructive azoospermic patients than that of other three groups. L-Alanine beta-naphthylamide hydrochloride was found to have highest hydrolysis rate in comparison to various other aminoacyl beta-naphthylamide hydrochlorides which were used for determining substrate specificity. 50% inhibitions of the enzyme activity by EDTA and 1,10-phenanthroline were obtained at 5.77 X 10(-3) M and 3.13 X 10(-6) M concentrations respectively. A single band for aminopeptidase in the activity - gel electrophoresis was obtained for all the categories of seminal plasma and the enzyme exhibited the characteristics of metalloprotein.
D000328:Adult; D000626:Aminopeptidases / Q000032:analysis*; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D008875:Middle Aged; D009845:Oligospermia / Q000201:enzymology; D012016:Reference Values; D012661:Semen / Q000201:enzymology*; D014659:Vasectomy
D004492:Edetic Acid; D000626:Aminopeptidases