Molecular mechanism for the production of multiple forms of MM creatine kinase.
Incubation of human, canine or rabbit MM creatine kinase with carboxypeptidase-N or B resulted in the production of 2 additional enzyme forms with increased anodal migration on polyacrylamide gels. The C-terminal amino acid of tissue MM creatine kinase from all 3 species was shown to be lysine, a specific substrate for carboxypeptidase-N and B.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D043424:Carboxypeptidase B; D002268:Carboxypeptidases / Q000378:metabolism; D003402:Creatine Kinase / Q000032:analysis / Q000378:metabolism*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007527:Isoenzymes; D008239:Lysine / Q000032:analysis; D001121:Lysine Carboxypeptidase / Q000378:metabolism; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D010446:Peptide Fragments / Q000032:analysis; D011817:Rabbits
D000596:Amino Acids; D007527:Isoenzymes; D010446:Peptide Fragments; D003402:Creatine Kinase; D002268:Carboxypeptidases; D043424:Carboxypeptidase B; D001121:Lysine Carboxypeptidase; D008239:Lysine