Induction of hepatic glutathione S-transferase activity by butylated hydroxyanisole and conjugation of benzo[a]pyrene diol-epoxide.
Dietary administration of 2(3)-tert-butyl-4-hydroxyanisole (BHA) to mice caused an increase in the hepatic soluble glutathione S-transferase activity towards (+/-)-7 beta, 8 alpha-dihydroxy-9 alpha, 10 alpha-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE) of approximately 5-fold whereas that towards 1-chloro-2,4-dinitrobenzene (CDNB) was increased by approximately 14-fold. Whereas with either substrate the catalytic capacity of the enzyme was elevated by BHA treatment, there was little effect on the Km for CDNB but an increase in the Km for BPDE as substrates. The results thus suggest that BHA-induced GSH S-transferase activity may be of limited importance for protection from certain reactive intermediates of polycyclic aromatic hydrocarbons.
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D000818:Animals; D000840:Anisoles / Q000633:toxicity*; D001580:Benzopyrenes / Q000633:toxicity*; D002083:Butylated Hydroxyanisole / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D003600:Cytosol / Q000201:enzymology; D004790:Enzyme Induction; D005260:Female; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D008815:Mice, Inbred Strains
D000840:Anisoles; D001580:Benzopyrenes; D002273:Carcinogens; D002083:Butylated Hydroxyanisole; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D005982:Glutathione Transferase