Effect of chemical modification on the cryoprecipitation of monoclonal human cryoglobulin M.
The effect of the chemical modification of lysine, histidine, arginine, tyrosine, tryptophan residues and carboxylic groups on the cryoproperties of monoclonal human cryoglobulin M has been studied. The modification of 35-40 lysine residues and that of 42-45 arginine residues in the molecule of cryo-IgM has been shown to result in practically complete inhibition of the cryoprecipitation. The same effect is observed on the modification of 60 histidine residues per molecule and on modification of 50 or 51 carboxylic groups. At the same time the modification of practically all the reagent-exposed tryptophan (10 residues per molecule) and tyrosine residues (55 residues per molecule) does not lead to any noticeable decrease in the cryoprecipitation. The conformations of the modified and native proteins are identical according to the circular dichroism data.
D000840:Anisoles; D001120:Arginine; D003080:Cold Temperature; D003450:Cryoglobulins / Q000302:isolation & purification*; D004047:Diethyl Pyrocarbonate; D005557:Formaldehyde; D006639:Histidine; D006801:Humans; D007075:Immunoglobulin M / Q000302:isolation & purification*; D008239:Lysine; D011485:Protein Binding; D014302:Trinitrobenzenesulfonic Acid; D014364:Tryptophan; D014443:Tyrosine; D008258:Waldenstrom Macroglobulinemia / Q000276:immunology*
D000840:Anisoles; D003450:Cryoglobulins; D007075:Immunoglobulin M; C043325:cryoglobulin M; D005557:Formaldehyde; C043324:2-methoxy-5-nitrobenzyl bromide; D014443:Tyrosine; D006639:Histidine; D014364:Tryptophan; D014302:Trinitrobenzenesulfonic Acid; D001120:Arginine; D008239:Lysine; D004047:Diethyl Pyrocarbonate