Measurement of prostaglandin D2 and identification of metabolites in human plasma during intravenous infusion.
A stable isotope dilution assay has been developed for the quantification of prostaglandin D2 (PGD2) in plasma. Samples are analysed by capillary column gas chromatography/negative ion chemical ionisation mass spectrometry (GC/NICIMS). The method employs an internal standard of [2H6]PGD2, prepared biosynthetically by incubation of rat peritoneal mast cells with deuterated arachidonic acid. No PGD2 was detected in peripheral venous plasma samples obtained from 10 healthy male volunteers (detection limit = 5 pg ml-1). Following intravenous infusion of PGD2 at increasing incremental infusion rates ranging from 16-256 ng kg-1 min-1, a dose related increase in the plasma concentration of PGD2 was observed. Plasma levels at 128 ng kg-1 min-1 ranged from 724-1444 pg ml-1. The major circulating metabolites of PGD2, during infusion, were identified as 13,14-dihydro-15-oxo-PGF2 alpha and 15-oxo-PGF2 alpha.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007263:Infusions, Parenteral; D007700:Kinetics; D008407:Mast Cells / Q000378:metabolism; D015230:Prostaglandin D2; D011457:Prostaglandins D / Q000008:administration & dosage / Q000096:biosynthesis / Q000097:blood*; D011865:Radioisotope Dilution Technique; D051381:Rats
D001095:Arachidonic Acids; D011457:Prostaglandins D; D016718:Arachidonic Acid; D003903:Deuterium; D015230:Prostaglandin D2