title: Immunohistochemical distribution of immunoglobulins, lactoferrin, and lysozyme in human minor salivary glands.
abstract: The immunofluorescence technique was used to examine the distribution of immunoglobulin A and its subclasses, secretory component (SC), J chain, lactoferrin and lysozyme in labial and lingual (von Ebner's) glands. IgA-containing plasma cells were found in the connective tissue around intercalated or intralobular ducts and a few were noted around acini of both glands. IgA was detected in the apical cytoplasm of intercalated and intralobular duct cells and in acini of von Ebner's glands and in demilunes of labial glands. Most IgA-containing cells also stained for J chain. The ratio of IgA1:IgA2-containing cells was approximately equal in von Ebner's and labial glands. Cytoplasmic and surface membrane-related staining for SC was detected in epithelial cells of the intercalated and intralobular ducts in both glands, in the serous acini of von Ebner's gland, and in the demilunes of labial glands. Lactoferrin was found in serous acini, demilunes, intercalated and intralobular ducts. Lysozyme was found in acinar and intercalated ducts, but was rarely seen in intralobular ducts. These results disclose the presence of cells (plasma cells and epithelial cells) and their products (IgA and secretory component) that indicate the local production of secretory IgA in minor salivary glands.
mesh_terms: D005455:Fluorescent Antibody Technique; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007144:Immunoglobulin J-Chains / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D007781:Lactoferrin / Q000032:analysis*; D007782:Lactoglobulins / Q000032:analysis*; D009113:Muramidase / Q000032:analysis*; D012469:Salivary Glands / Q000032:analysis*; D012470:Salivary Glands, Minor / Q000032:analysis* / Q000166:cytology / Q000201:enzymology / Q000276:immunology
chemical_list: D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D007144:Immunoglobulin J-Chains; D007136:Immunoglobulins; D007782:Lactoglobulins; D009113:Muramidase; D007781:Lactoferrin