Separation of rat testis cells by a manually operated countercurrent distribution apparatus. I. Partition behaviour of cells in aqueous two-phase system.
The partition behaviour of rat testis cells in an aqueous two-phase polymer system has been studied using a simplified version of the countercurrent distribution apparatus. The influence of the phase system on testis cells has been presented. The manually operated apparatus designed has shown a fair enrichment of cell types, depending on their surface properties. In respect to their developmental stages, different cell types have shown their specific rate of migration through the CCD tubes. Non-germinal cells, mainly the Sertoli cells, have been seen to have a higher migration rate than the spermatids and spermatocytes. The pachytene spermatocytes have the lowest migration rate.
D000818:Animals; D002465:Cell Movement; D002469:Cell Separation / Q000379:methods; D003377:Countercurrent Distribution / Q000295:instrumentation; D004492:Edetic Acid; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000166:cytology*; D014357:Trypsin
D004492:Edetic Acid; D014357:Trypsin