Lectin-binding in premalignant lesions during submandibular gland carcinogenesis.
Complex carbohydrates in premalignant lesions of mouse submandibular gland tumors were examined by the lectin-peroxidase conjugate method. Peroxidase-conjugated lectins of PNA, RCA-1, DBA, SBA, UEA-1 and WGA were used to detect specific sugar residues of complex carbohydrates in premalignant lesions during experimental carcinogenesis. Marked reduction of PNA and SBA bindings occurred in duct-like structures and cystic lesions which were transformed from granular convoluted tubule cells. Premalignant lesions bound slightly to PNA, RCA-1, DBA, SBA and WGA and manifested increased UEA-1 binding. Squamous metaplastic epithelia of premalignant lesions manifested increased binding to PNA, RCA-1 and SBA as compared to those of duct-like structure and cystic epithelia.
D000606:Amino Sugars / Q000494:pharmacology; D000818:Animals; D050260:Carbohydrate Metabolism; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D006601:Hexoses / Q000494:pharmacology; D037102:Lectins; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology; D011230:Precancerous Conditions / Q000378:metabolism* / Q000473:pathology; D011975:Receptors, Mitogen / Q000378:metabolism; D012468:Salivary Gland Neoplasms / Q000378:metabolism*; D013365:Submandibular Gland Neoplasms / Q000378:metabolism* / Q000473:pathology
D000606:Amino Sugars; D006601:Hexoses; D037102:Lectins; D011975:Receptors, Mitogen