High frequency of lambda gene activation in bone marrow pre-B cells.
The frequency of lambda light chain (L) producing cells in immunoglobulin-producing cells that have been generated in vitro from bone marrow pre-B cells was investigated. The frequency of lambda-producing cells obtained in such a culture was three- to eightfold higher than that observed in the culture of mature spleen B cells. These results suggest that the activation of lambda gene at pre-B cell stage occurs far more frequently than the frequency presumed from the percentage of lambda-bearing cells in mature B cells.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D006462:Hemolytic Plaque Technique; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D007146:Immunoglobulin lambda-Chains / Q000235:genetics* / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013234:Stem Cells / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains