Involvement of protein HMG1 in DNA replication.
Antibodies against HMG1 inhibit the incorporation of [3H]thymidine in Ehrlich ascites cell nuclei. By the use of specific inhibitors it is shown that HMG1 is needed for the action of the replicative DNA polymerase and not for the reparative one. This is supported by the fact that the addition of exogenous HMG1 to the nuclei enhances the replication process.
D000818:Animals; D004261:DNA Replication; D004797:Enzyme-Linked Immunosorbent Assay; D005033:Ethylmaleimide / Q000494:pharmacology; D006609:High Mobility Group Proteins / Q000502:physiology*; D005779:Immunodiffusion; D008297:Male; D011817:Rabbits; D013696:Temperature; D013942:Thymine Nucleotides / Q000378:metabolism
D006609:High Mobility Group Proteins; D013942:Thymine Nucleotides; D005033:Ethylmaleimide; C024157:thymidine 5'-triphosphate