Hemagglutination and the closest distance of approach of normal, neuraminidase- and papain-treated erythrocytes.
By means of potential energy versus distance diagrams, derived from electrophoretic and surface tension data, the minimum distances of approach of normal (NOR) and of neuraminidase-treated (NEU) and papain-treated (PAP) human erythrocytes could be determined. The minimum distances between the actual cell membranes of two opposing red cells are: 184 A (NOR), 111 A (NEU), and 113 A (PAP), which agrees well with the fact that anti-D (Rho) antibodies of the IgG-class (which have a maximum distance of approximately 120 A between the two antibody-active sites) can hemagglutinate NEU and PAP cells, but are incapable of hemagglutinating normal D (Rho)-positive erythrocytes.
D000937:Antigen-Antibody Reactions; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004734:Energy Metabolism; D004910:Erythrocyte Membrane / Q000276:immunology; D004912:Erythrocytes / Q000187:drug effects; D006384:Hemagglutination; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007518:Isoantibodies / Q000276:immunology; D009439:Neuraminidase / Q000494:pharmacology; D010206:Papain / Q000494:pharmacology; D012204:Rh-Hr Blood-Group System / Q000276:immunology; D013499:Surface Properties
D007074:Immunoglobulin G; D007518:Isoantibodies; C033242:RH-antibodies; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen; D009439:Neuraminidase; D010206:Papain