Anti-phosphorylcholine antibodies with a preferred reactivity for either PC or PC-phenyl represent independent expressions.
Anti-hapten antibodies to phosphorylcholine(PC)-conjugated proteins can be divided into antibodies reacting preferentially with PC (group I) or with PC-phenyl (group II). We have selected two hybridomas producing group II-type anti-PC antibodies of the IgM and IgE classes, respectively. With one exception, NH2-terminal amino acid sequence analyses of the light chains are identical in their first 21 residues and similar to that of M460, but differ from that of group I-type light chains. These results suggest that PC-phenyl-specific group II antibodies are expressed independently of PC-specific group I antibodies.
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001555:Benzene Derivatives / Q000276:immunology; D002460:Cell Line; D002794:Choline / Q000031:analogs & derivatives*; D006825:Hybridomas / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D010767:Phosphorylcholine / Q000276:immunology*
D000906:Antibodies; D001555:Benzene Derivatives; D007147:Immunoglobulin Light Chains; D010767:Phosphorylcholine; D002794:Choline