Tyrosinase-like activity in normal human substantia nigra.
A tyrosinase-like activity was found in human substantia nigra by polyacrylamide gel electrophoresis of fractions prepared from homogenates of the substantia nigra. The enzyme activity was detected by staining the gels with L-3,4-dihydroxyphenylalanine, dopamine and 5,6-dihydroxyindole as substrates for tyrosinase (EC 1.14.18.1). A case of parkinsonism does not show the L-3,4-dihydroxyphenylalanine and dopamine oxidase activities.
D000368:Aged; D001923:Brain Chemistry; D004156:Catechol Oxidase / Q000378:metabolism*; D002648:Child; D004295:Dihydroxyphenylalanine / Q000032:analysis; D004298:Dopamine / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007211:Indoles / Q000032:analysis; D008297:Male; D008875:Middle Aged; D014442:Monophenol Monooxygenase / Q000032:analysis / Q000378:metabolism*; D010300:Parkinson Disease / Q000201:enzymology; D010670:Phenylthiourea / Q000032:analysis; D013378:Substantia Nigra / Q000032:analysis / Q000201:enzymology*
D007211:Indoles; D004295:Dihydroxyphenylalanine; D010670:Phenylthiourea; D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase; D004298:Dopamine; C033871:5,6-dihydroxyindole