Induction of sister chromatid exchanges by mitomycin C in lymphocytes of young and old human donors.
The kinetics of cell proliferation and sister chromatid exchanges (SCE) were studied in blood lymphocytes treated by mitomycin C (MMC) in young and old human donors. In both the young and old donors MMC inhibited cell proliferation. Spontaneous SCE frequency was not age-dependent. In young donors, MMC induced a dose-related linear increase in SCE frequency. In old donors a significantly reduced SCE rate was induced by 80 ng/ml of MMC, suggesting an altered SCE response to mutagen. As SCE represent some form of DNA repair, the results can be interpreted as an indication of a defective DNA repair in aging human lymphocytes.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects; D008677:Metaphase / Q000187:drug effects; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology*; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D000903:Antibiotics, Antineoplastic; D008937:Mitomycins; D016685:Mitomycin