Radiosensitivity of non-primed and primed human T cells in vitro.
Primary and secondary proliferative responses to hapten (TNP)-modified autologous cells as well as to allogeneic cells, antigen (purified protein derivative) and mitogens were used for in vitro studies of the radiosensitivity of human T cells. To be able to compare data obtained from the various assay systems, optimal responding/stimulating cell ratios were used and the post-irradiation period kept constant. With these precautions, no difference in radiosensitivity of the various T cell responses could be found whether they were induced by mitogen, antigen, allogeneic cells or TNP-modified autologous cells. Neither could, on a per-cell basis, any difference in radiosensitivity between primed and non-primed cells be detected. At 400 rad, about 10% of the response was recovered in either case. The immunocompetence of the T cells surviving irradiation, as estimated by the ability to give a TNP-specific response, was not impaired.
D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000528:radiation effects*; D013997:Time Factors; D014301:Trinitrobenzenes / Q000276:immunology; D014373:Tuberculin / Q000276:immunology; D014965:X-Rays
D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D014301:Trinitrobenzenes; D014373:Tuberculin