Reappraisal of the mucosal epithelial space associated with the surface of Hymenolepis diminuta and its effect on transport parameters.
Using nonpermeating, radiolabeled solutes to estimate the magnitude of the "unstirred water layer" (="mucosal epithelial space") of the surface of Hymenolepis diminuta, a value approximating 1% of the worm's fluid volume (0.011-0.022 ml/g wet tissue) was obtained. This value was compared with those previously reported by other workers which were greater by an order of magnitude. The difference between these results appears to be related to the use in previous studies of a permeating marker (mannitol), and a failure to divest the surface of nonspecifically adherent bathing fluid in excess of the actual "unstirred layer". These parameters must be considered in future studies on this useful model for the study of transport.
D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D006926:Hymenolepis / Q000378:metabolism* / Q000648:ultrastructure; D007444:Inulin / Q000378:metabolism; D008353:Mannitol / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism; D010042:Ouabain / Q000378:metabolism; D010695:Phlorhizin / Q000378:metabolism
D008353:Mannitol; D010042:Ouabain; D007444:Inulin; D010695:Phlorhizin