Allogeneic radiation chimeras respond to TNP-modified donor and host targets.
Tolerance to major histocompatibility antigens as well as the ability to mount a cytotoxic response to hapten-modified cells of bone marrow donor and host origin was studied in allogeneic radiation chimeras. Lethally irradiated (C57BL/6 X DBA/2)F1 hosts reconstituted with anti-Thy 1.2 + C-treated bone marrow from (C57BL/6 X CBA)F1 mice showed tolerance to the MHC antigens of the three parental strains as measured by MLC and CML assay. The chimeras responded normally to unrelated allogeneic cells. Chimeric animals generated a cytotoxic response to hapten-modified cells of both donor (CBA) and host (DBA/2) haplotypes, as well as to C57BL/6, demonstrating that tolerance to the hapten-presenting host haplotype is sufficient to allow a cytotoxic antihapten response, and that processing through a semiallogeneic host environment does not affect the ability to generate a response to hapten in conjunction with self-determinants. Chimeras failed to mount a cytotoxic response to hapten presented on nontolerated allogeneic spleen cells.
D000818:Animals; D001667:Binding, Competitive; D001853:Bone Marrow / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005260:Female; D006238:Haploidy; D007106:Immune Sera / Q000494:pharmacology; D007108:Immune Tolerance; D007959:Lymphocyte Culture Test, Mixed; D008285:Major Histocompatibility Complex; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D009578:Nitrobenzenes / Q000276:immunology*; D011828:Radiation Chimera; D014301:Trinitrobenzenes / Q000276:immunology*
D007106:Immune Sera; D009578:Nitrobenzenes; D014301:Trinitrobenzenes