[Dynamics and distribution of T- and B-dependent lymphocytes in the lymphoid organs of guinea pigs in experimental ascariasis].
After experimental invasion of guinea-pigs with the eggs of Ascaris suum (5,000 eggs per animal), the dynamics and distribution of T and B-dependent cells in lymphoid organs (spleen, mesenteric, hepatic and mediastinal nodes) were studied. The detection of both cell populations by means of rosette methods (E, EA, EAC) showed that in all studied organs their first increase took place on the first and second day after invasion. On the fifth to seventh day after invasion the maximum increase in T cells was observed in spleen, mesenteric and hepatic nodes and on the ninth day in mediastinal nodes. After an initial increase, the number of B cells carrying specific receptors for Fc fragment Ig (EA-rosettes) reached its maximum in mesenteric nodes on the fifth day, in spleen on the seventh day and in hepatic and mediastinal nodes on the ninth day after invasion. The maximum number of B cells with receptors for complement (EAC-rosettes) appeared on the seventh day after invasion in spleen and in hepatic and mesenteric nodes, whereas in mediastinal nodes on the ninth day. On the 21st day after invasion the percentage of T and B cells decreased to the initial values. As follows from the results, the dynamics and distribution of T and B-dependent cells, detected in corresponding organs by rosette methods, correlated with the ascaroid type of endogenous migration of Ascaris suum larvae.
D000818:Animals; D001196:Ascariasis / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D007958:Leukocyte Count; D008221:Lymphoid Tissue / Q000276:immunology / Q000473:pathology; D008297:Male; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*
Dynamika a distribúcia T a B dependentných lymfocytov v lymfoidných orgánoch morciat pri experimentálnej askaridóze.