Effect of size of Trichinella spiralis (Nematode) infections on glucose and ion transport in the rat intestine.
An in vivo perfusion technique, using 3 intestinal loops representing the anterior, mid and posterior regions of the rat small intestine, was used to determine intestinal glucose uptake 5 days after infection with Trichinella spiralis. At high levels of infection (3,000 and 6,000 larvae/rat) net glucose absorption by the intestinal mucosa was significantly impaired in all regions of the small intestine when compared to uninfected controls. At low levels of infection (50 larvae/rat) glucose uptake by the mucosa was significantly enhanced in all 3 regions of the small intestine. Intermediate levels of infections (200-1,000 larvae/rat) also enhanced glucose uptake, but only in the anterior regions of the small intestine. When washings from the small intestine of rats infected with 50 larvae/rat were added to the perfusion fluid used on uninfected rats, glucose uptake was also significantly enhanced. These results suggest that at low levels of infection the intestinal lumen contains a metabolite which may affect the mucosal transport of glucose and the related fluxes of H2O, Na+, Cl-, and K+, in the rat intestine. Luminal [H+] and pCO2 decreased from the proximal to distal regions of the small intestine following perfusion; pO2 was significantly decreased in the proximal and distal regions.
D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D001692:Biological Transport; D002245:Carbon Dioxide / Q000032:analysis; D002412:Cations / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000032:analysis / Q000378:metabolism*; D010100:Oxygen / Q000032:analysis; D011188:Potassium / Q000378:metabolism; D051381:Rats; D012964:Sodium / Q000378:metabolism; D014234:Trichinella / Q000502:physiology*; D014235:Trichinellosis / Q000378:metabolism* / Q000469:parasitology
D001639:Bicarbonates; D002412:Cations; D002712:Chlorides; D002245:Carbon Dioxide; D012964:Sodium; D005947:Glucose; D011188:Potassium; D010100:Oxygen