Inhibitory effects of alpha- and beta-naphthoflavones on DMBA-induced anomalies in germ cells assessed by sperm abnormality assay.
Frequencies of abnormal sperms in B6C3F1 male mice were analyzed after injection with 7,12-dimethylbenz[a]anthracene (DMBA), alpha-naphthoflavone (alpha-NF or 7,8-benzoflavone), beta-naphthoflavone (beta-NF or 5,6-benzoflavone) and combinations of either alpha-NF and DMBA or beta-NF and DMBA. Either alpha-NF or beta-NF was injected 48 and 24 h before injecting mice twice with DMBA for 2 days at 24-h intervals. Prior injection of mice with alpha-NF and beta-NF was based on the assumption that enhanced activity of monooxygenase enzymes (P-450) in the mouse system so as to modify the metabolism of DMBA towards increased detoxification. Both flavones showed inhibitory effects in the genotoxic action of DMBA to the extent of 83% reduction by alpha-NF and 60% by beta-NF in the number of abnormal sperms as compared with those found in germ cells due to DMBA alone. compared with those found in germ cells due to DMBA alone.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000037:antagonists & inhibitors*; D000818:Animals; D001551:Benz(a)Anthracenes / Q000037:antagonists & inhibitors*; D001571:Benzoflavones / Q000494:pharmacology*; D004347:Drug Interactions; D005419:Flavonoids / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009154:Mutation / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects; D019324:beta-Naphthoflavone
D001551:Benz(a)Anthracenes; D001571:Benzoflavones; D005419:Flavonoids; D015127:9,10-Dimethyl-1,2-benzanthracene; C011512:alpha-naphthoflavone; D019324:beta-Naphthoflavone