Rapid characterization and partial purification of various animal amine oxidases.
The use of chromatofocusing to obtain a rapid characterization of tissue amine oxidases from various mammals is proposed. This technique yields partially purified enzymes well suited for immunological studies. Chromatofocusing can be also used in a three-step purification of pig kidney diamine oxidase.
D006631:Amine Oxidase (Copper-Containing) / Q000302:isolation & purification; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D006801:Humans; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D007668:Kidney / Q000201:enzymology; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000302:isolation & purification*; D013045:Species Specificity; D013552:Swine
D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors