[Possibilities for the use of cerium for the ultrahistochemical demonstration of enzymes].
The activity pattern of NAD(H)- or NADP(H)-oxidase was studied ultrahistochemically by means of the Cer-III-perhydroxide technique according in Briggs et al. (1979) in erythrophagocytosis. In contrast to many other findings in activated polymorph nucleated neutrophils the activity of this enzyme is restricted to rER and the intracellular vacuoles in peritoneal macrophages. Plasmalemma and the membranes of phagosomes are negative. The results allow the conclusion that oxidases do not directly participate in erythrocyte catabolism. Their role is still unclear. Additionally an ultrahistochemical procedure which used Cer-III-=ions as capture agent for detection of acid phosphatase is presented. The results are compared with the classical lead methods. Cer-IV-ions selectively enhance the electronmicroscopic contrast of biomembranes.
D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D002563:Cerium; D006651:Histocytochemistry / Q000379:methods*; D007668:Kidney / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology; D008264:Macrophages / Q000201:enzymology; D008854:Microscopy, Electron; D009097:Multienzyme Complexes / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010587:Phagocytosis; D051381:Rats
D009097:Multienzyme Complexes; D002563:Cerium; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D000135:Acid Phosphatase
Zur Verwendungsmöglichkeit von Cer für den ultrahistochemischen Nachweis von Enzymen.