An Escherichia coli replication protein that recognizes a unique sequence within a hairpin region in phi X174 DNA.
Protein n', a prepriming DNA replication enzyme of Escherichia coli, is a phi X174 DNA-dependent ATPase. Restriction of phi X174 DNA have led to the identification of a 55-nucleotide fragment that carries the protein n' recognition sequence. Molecular hybridization and sequence analysis have located this sequence within the untranslated region between genes F and G, a map location analogous to that of the unique complementary strand origin of phage G4 DNA. Within the 55-nucleotide fragment is a sequence of 44 nucleotides that forms a stable hairpin structure. This duplex may be the signal for protein n' to initiate the prepriming events that led to the start of phi X174 complementary DNA strand replication.
D001426:Bacterial Proteins / Q000378:metabolism*; D010584:Bacteriophage phi X 174 / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000378:metabolism*; D005814:Genes, Viral; D006860:Hydrogen Bonding; D009690:Nucleic Acid Conformation; D014779:Virus Replication
D001426:Bacterial Proteins; D004279:DNA, Viral