[Purification of human cartilaginous proteoglycans and technical aspects of the radioimmunoassay].
Proteoglycans (PG) have been purified by classical methods from human articular cartilage in order to set up a radioimmunoassay. Conditions of labelling, purification of labelled PG, and optimal conditions of buffer, temperature, duration of incubations and dilution of antiserum are described. Separation of free and bound PG is performed by immunoprecipitation. It is demonstrated that human articular PG can be assayed quantitatively by RIA procedure, with the sensitivity of +/- 2 femto-moles (+/- 5 ng) per tube.
D000818:Animals; D002358:Cartilage, Articular / Q000032:analysis*; D006801:Humans; D011509:Proteoglycans / Q000302:isolation & purification*; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods
D011509:Proteoglycans
Purification de protéoglycannes cartilagineux humains et aspects techniques du dosage radioimmunologique.