[Photochemical affinity labeling of the macrolide binding site on the 70S E. coli ribosome].
Photoactivation of the alpha, beta-unsaturated ketone-epoxide system of [3H] dihydrorosaramycin at a wavelength above 300 nm allows the covalent attachment of the antibiotic to its receptor site. The radioactivity is mainly associated to proteins L1, L5, L6, S1; as a consequence, the binding site of this type of drug could be located at the peptidyltransferase center and in between both subunits.
D000345:Affinity Labels; D001665:Binding Sites; D004926:Escherichia coli / Q000378:metabolism* / Q000648:ultrastructure; D007933:Leucomycins / Q000378:metabolism*; D010777:Photochemistry; D012270:Ribosomes / Q000378:metabolism*; D014316:Tritium
D000345:Affinity Labels; D007933:Leucomycins; D014316:Tritium
Marquage d'affinité photochimique du site de fixation des macrolides sur le ribosome 70S d'E. coli.