[Energy migration in phycobilisomes].
Fluorescence emission and polarization spectra of the phycobilisomes (PBS) of the blue-green alga Nostoc muscorum were measured at 20, -73 and -196 degrees C while exciting at the absorption maximum of each pigment in the PBS. The emission spectra were deconvoluted into a number of Gaussian components and energy migration coefficients and quantum yields of fluorescence for the 8 forms of the phycobilins constituting the PBS were calculated. The overlap integrals and the critical and real distances for the energy transfer in the donor-acceptor pairs were evaluated. The general scheme of the energy transfer in the PBS is proposed according to which there is a homogeneous energy migration within each pigment form and a following effective heterogeneous migration directed from the short wavelength forms via the intermediate ones to the terminal long wavelength acceptors. The transfer passes one or more steps of the energy "staircase" which is formed by the excited levels of the forms. The backward "uphill" energy transfer does not take place. These data and the estimates of the real distances of the energy transfer allowed us to make a conclusion on the regular arrangement of the pigments in the PBS, to determine the distances between the chromophores and their localization in a pigment molecule and the distances between the chromophores of different pigments and thus to specify the structure of the PBS.
D000458:Cyanobacteria / Q000378:metabolism*; D004579:Electron Transport; D004734:Energy Metabolism; D005453:Fluorescence; D045342:Light-Harvesting Protein Complexes; D008954:Models, Biological; D045524:Phycobilisomes; D010798:Phycocyanin / Q000378:metabolism; D010799:Phycoerythrin / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D013057:Spectrum Analysis
D045342:Light-Harvesting Protein Complexes; D045524:Phycobilisomes; D010940:Plant Proteins; D010798:Phycocyanin; D010799:Phycoerythrin
Migratsiia énergii vozbuzhdeniia v fikobilisomakh.