Application of high-performance liquid chromatography with synchronized accumulating radioisotope detector to analysis of glyceryl trinitrate and its metabolites in rat plasma.
A new, sensitive, and specific high-performance liquid chromatographic method for the quantitative analysis of [14C]glyceryl trinitrate and its four metabolites in plasma is described. The drugs are extracted from 0.05 ml of plasma with methanol and analyzed by high-performance liquid chromatography using a synchronized accumulating radioisotope detector. The limit of detection is 0.2 ng per injection. The within-day coefficient of variation is 5.9% at a concentration of 27.0 ng per ml of plasma. The method was applied to single-dose pharmacokinetics of glyceryl trinitrate in rat.
D000818:Animals; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006207:Half-Life; D007275:Injections, Intravenous; D007700:Kinetics; D008297:Male; D005996:Nitroglycerin / Q000008:administration & dosage / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains
D002250:Carbon Radioisotopes; D005996:Nitroglycerin