Identification and purification of a 55K polypeptide in Sjögren's syndrome A antigen.
The Sjögren's syndrome A antigen (SS-A) purified by affinity chromatography from human spleen was shown to contain a major polypeptide with a mol. wt. of 55 000 daltons confirmed as SS-A by its reactivity with different anti-SS-A sera on affinity columns, immunodiffusion and the Western blot. A similar reactive polypeptide was demonstrated in extracts of rabbit liver, brain, spleen, kidney, lung and thymus.
D000818:Animals; D000941:Antigens / Q000032:analysis / Q000302:isolation & purification*; D001324:Autoantigens; D002846:Chromatography, Affinity; D006801:Humans; D005779:Immunodiffusion; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification*; D020733:RNA, Small Cytoplasmic; D011817:Rabbits; D012261:Ribonucleoproteins; D012859:Sjogren's Syndrome / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D000099207:SS-A Antigen
D000941:Antigens; D001324:Autoantigens; D010455:Peptides; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human