Uptake of arachidonic acid, arachidic acid, oleic acid and their incorporation into phospholipids and triacylglycerols of isolated murine hepatocytes. Effect of thrombin-antithrombin III complex.
Uptake and metabolism of arachidonic acid, arachidic acid and oleic acid were investigated in isolated hepatocytes prepared from mouse liver with the collagenase perfusion method. The rate of uptake of arachidonic acid was time- and concentration- dependent. 94-98% of the arachidonic acid was incorporated into the phospholipid and triacylglycerol fractions following a 60 min incubation period at 37 degrees C. In the presence of thrombin-anti-thrombin III complex a change in the distribution of arachidonic acid incorporated into lipid fractions was found, i.e. increased incorporation into phosphatidyl-serine and phosphatidylethanolamine, whereas the uptake was not altered. There was no change in the uptake and incorporation of arachidic acid and oleic acid.
D000818:Animals; D000990:Antithrombin III / Q000494:pharmacology*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004537:Eicosanoic Acids / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D013917:Thrombin / Q000494:pharmacology*; D014280:Triglycerides / Q000378:metabolism*
D001095:Arachidonic Acids; D004537:Eicosanoic Acids; D009829:Oleic Acids; D010743:Phospholipids; D014280:Triglycerides; D016718:Arachidonic Acid; D019301:Oleic Acid; D000990:Antithrombin III; D013917:Thrombin