Serum, bradykinin and vasopressin stimulate release of inositol phosphates from human fibroblasts.
The mitogens serum, vasopressin and bradykinin stimulate a significant rise in the inositol phosphate content of cultured human fibroblasts within 10 seconds, while serum- and bradykinin-stimulated arachidonic acid release does not occur until after 30 seconds. The release of inositol phosphates is not secondary to a rise in Ca activity since the Ca ionophore ionomycin does not stimulate release of inositol phosphates. Moreover, we show that phospholipase C in human fibroblasts is activated by these mitogens at resting Ca levels since TMB-8, which blocks the mitogen-induced rise in Ca activity, does not affect the serum-stimulated accumulation of inositol phosphates.
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001769:Blood; D001920:Bradykinin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D004987:Ethers / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism*; D015759:Ionomycin; D013403:Sugar Phosphates / Q000378:metabolism*; D013997:Time Factors; D010738:Type C Phospholipases / Q000378:metabolism; D014667:Vasopressins / Q000494:pharmacology*
D001095:Arachidonic Acids; D004987:Ethers; D007295:Inositol Phosphates; D013403:Sugar Phosphates; D014667:Vasopressins; D016718:Arachidonic Acid; D015759:Ionomycin; D010738:Type C Phospholipases; D001920:Bradykinin; D002118:Calcium