The effects of aspirin and OKY-1581 on the metabolism of exogenous arachidonic acid in rat alveolar macrophages.
When non-stimulated rat alveolar macrophages were incubated with exogenous 14C-arachidonic acid (7 microM) both cyclo-oxygenase and lipoxygenase metabolites were formed. The major cyclo-oxygenase metabolites were PGE2, PGF2 alpha and TXB2. The detected amounts of 5-lipoxygenase metabolites were small. Aspirin inhibited the formation of all cyclo-oxygenase metabolites, but had no effect on the formation of the lipoxygenase metabolites. In the presence of OKY-1581, an inhibitor of thromboxane synthetase, the formation of thromboxane B2 was decreased and that of the other cyclo-oxygenase metabolites was correspondingly increased. The present study indicates that OKY-1581 is a specific inhibitor of thromboxane synthetase also in rat alveolar macrophages and that the rate of formation of leukotrienes via the 5-lipoxygenase pathway is rather small in non-stimulated rat alveolar macrophages.
D000179:Acrylates / Q000494:pharmacology*; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D001241:Aspirin / Q000494:pharmacology*; D015237:Dinoprost; D015232:Dinoprostone; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008689:Methacrylates / Q000494:pharmacology*; D010088:Oxidoreductases / Q000037:antagonists & inhibitors*; D011458:Prostaglandins E / Q000096:biosynthesis; D011460:Prostaglandins F / Q000096:biosynthesis; D011650:Pulmonary Alveoli / Q000166:cytology*; D051381:Rats; D013929:Thromboxane B2 / Q000096:biosynthesis; D013930:Thromboxane-A Synthase / Q000037:antagonists & inhibitors*
D000179:Acrylates; D001095:Arachidonic Acids; D008689:Methacrylates; D011458:Prostaglandins E; D011460:Prostaglandins F; D016718:Arachidonic Acid; D013929:Thromboxane B2; C031517:2-methyl-3-(4-(3-pyridinylmethyl)phenyl)-2-propenoic acid; D015237:Dinoprost; D010088:Oxidoreductases; D013930:Thromboxane-A Synthase; D015232:Dinoprostone; D001241:Aspirin