Separation of four glucuronides in a single sample by high-pressure liquid chromatography and its use in the determination of UDP glucuronosyltransferase activity toward four aglycones.
A single-solvent, reverse-phase HPLC system that separates glucuronides has been developed, and its applicability to the separation of glucuronides of four substrates (1-naphthol, morphine, testosterone, and estrone), representative of different UDP-glucuronosyltransferase enzymes, has been demonstrated. The combined supernatants from four separate microsomal incubations from which the protein has been precipitated can be used directly for the quantitation of the glucuronides formed. Variations in the four enzyme activities, upon treatment of rats with microsomal enzyme-inducing agents, after liver trauma and in the Gunn strain of rats have been investigated.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004970:Estrone / Q000378:metabolism; D005965:Glucuronates / Q000302:isolation & purification*; D014453:Glucuronosyltransferase / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D009020:Morphine / Q000378:metabolism; D009284:Naphthols / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012997:Solvents; D013379:Substrate Specificity; D013739:Testosterone / Q000378:metabolism
D005965:Glucuronates; D009284:Naphthols; D012997:Solvents; C029350:1-naphthol; D004970:Estrone; D013739:Testosterone; D009020:Morphine; D014453:Glucuronosyltransferase