Calcium stimulation of a novel 12-lipoxygenase from rat basophilic leukemia (RBL-1) cells.
Cytosolic fraction of RBL-1 cells transformed arachidonic acid to 12-HETE in addition to the well-recognized 5-hydroxyeicosatetraenoic acid (5-HETE) in the presence of Ca2+, Mg2+ or Mn2+. The identity of 12-HETE was confirmed by gas chromatography-mass spectrometry. Syntheses of 12-HETE and 5-HETE were catalyzed by separate lipoxygenases, since the formation of each product showed differential sensitivity to inhibitors and temperature. 12-Lipoxygenase from RBL-1 cells was also found to be distinct from the enzyme from platelets in calcium sensitivity.
D000818:Animals; D001091:Arachidonate Lipoxygenases; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001491:Basophils / Q000201:enzymology*; D001792:Blood Platelets / Q000201:enzymology; D002118:Calcium / Q000494:pharmacology*; D006358:Hot Temperature; D007093:Imidazoles / Q000494:pharmacology; D007938:Leukemia / Q000201:enzymology; D008084:Lipoxygenase / Q000378:metabolism*; D016859:Lipoxygenase Inhibitors; D051381:Rats; D014468:Umbelliferones / Q000494:pharmacology
D001095:Arachidonic Acids; D007093:Imidazoles; D016859:Lipoxygenase Inhibitors; D014468:Umbelliferones; D016718:Arachidonic Acid; D001091:Arachidonate Lipoxygenases; D008084:Lipoxygenase; C007628:esculetin; D002118:Calcium; C012160:N-acetylimidazole