title: Isotype of lytic antibodies in serum of Chagas' disease patients.
abstract: The isotype of anti-Trypanosoma cruzi lytic antibodies present in sera obtained from patients with the chronic phase of Chagas' disease was determined. Serum samples were submitted to gel filtration in Sephacryl S-200, ion exchange chromatography in DEAE-cellulose, affinity chromatography with protein A and immunoadsorption with anti-human IgG-Sepharose. The results of these experiments showed that human anti-T. cruzi lytic antibodies are mainly located in the IgG isotype probably in the IgG1 and IgG2 subclasses.
mesh_terms: D014355:Chagas Disease / Q000276:immunology*; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007126:Immunoglobulin Allotypes / Q000032:analysis*; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D013205:Staphylococcal Protein A; D014349:Trypanosoma cruzi / Q000276:immunology
chemical_list: D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013205:Staphylococcal Protein A