[Fluorescence decay curves under pulsed excitation: distinction between free and bound NAD(P)H on isolated living cells].
Under pulsed excitation the decay curve of the intrinsic cellular fluorescence has been recorded using an original equipment. Cell by cell experiments are feasible so that the NAD(P)H bound/free ratio and the relaxation time characteristic of bound NAD(P)H can be used in order to build up histograms characterizing the cell population.
D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D051379:Mice; D009249:NADP / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods
D009249:NADP
Courbes de déclin de la fluorescence excitée en régime pulsé: distinction du NAD(P)H libre et du NAD(P)H lié sur des cellules vivantes isolées.