Construction and characterization of Escherichia coli polA-lacZ gene fusions.
The promoter of the polA gene of Escherichia coli K-12 was fused to the lacZ gene by selecting deletions within a lambda lacZ polA transducing phage. Four fusions, deleting varying amounts of the polA gene, were characterized. The polA promoter was found to be approximately 3% as active as the fully induced lac promoter. This figure is compatible with the normal intracellular level of deoxyribonucleic acid polymerase I. No evidence was found for outogenous regulation of transcription from the polA promoter. Expression from this promoter was influenced by neither recA nor mitomycin C, but uvrD and uvrE mutations reduced expression slightly.
D010582:Bacteriophage lambda / Q000235:genetics; D004256:DNA Polymerase I / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D004274:DNA, Recombinant; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D007763:Lac Operon; D008937:Mitomycins / Q000494:pharmacology; D009692:Nucleic Acid Heteroduplexes / Q000032:analysis; D009876:Operon; D011995:Recombination, Genetic; D014466:Ultraviolet Rays; D001616:beta-Galactosidase / Q000096:biosynthesis
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D008937:Mitomycins; D009692:Nucleic Acid Heteroduplexes; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase; D001616:beta-Galactosidase