Determination of human apolipoproteins A-I, B, and E by laser nephelometry.
A laser immunonephelometric procedure for the determination of human apolipoproteins A-I, B, and E in serum and lipoproteins was developed. Coefficients of variation were 2.3%-3.4% within-run, 4.7%-4.9% between-run. Serum apolipoprotein A-I values were similar in normal and hyperlipoproteinaemic subjects with a correlation coefficient of r = 0.585 to HDL-cholesterol levels. LDL-apolipoprotein B values were highly (r = 0.935), VLDL-apolipoprotein B values less strongly (r = 0.472) correlated to the corresponding cholesterol levels. Serum apolipoprotein E concentrations were distinctly elevated in Type III and not correlated to cholesterol values.
D000328:Adult; D000368:Aged; D000906:Antibodies / Q000032:analysis; D016632:Apolipoprotein A-I; D001053:Apolipoproteins / Q000097:blood*; D001055:Apolipoproteins B; D001057:Apolipoproteins E; D002784:Cholesterol / Q000097:blood; D006801:Humans; D007118:Immunoassay; D008027:Light; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry / Q000379:methods; D012542:Scattering, Radiation
D000906:Antibodies; D016632:Apolipoprotein A-I; D001053:Apolipoproteins; D001055:Apolipoproteins B; D001057:Apolipoproteins E; D002784:Cholesterol