title: A method for the synthesis of [14C]-kynurenine.
abstract: A new method is described for the synthesis of [14C]-labelled L-kynurenine from [14C]-L-tryptophan, using extracts of tryptophan-adapted cells of Pseudomonas marginalis. It is based on the selective, rapid inactivation of kynureninase by a newly discovered inhibitor of this enzyme, 3-chloro-L-alanine. The yield of [14C]-kynurenine produced in this manner is 76% theoretical.
mesh_terms: D006867:Hydrolases / Q000037:antagonists & inhibitors / Q000378:metabolism; D007737:Kynurenine / Q000096:biosynthesis*; D011549:Pseudomonas / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014364:Tryptophan / Q000378:metabolism; D015091:beta-Alanine / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D015091:beta-Alanine; D007737:Kynurenine; C006977:3-chloroalanine; D014364:Tryptophan; D006867:Hydrolases; C021480:kynureninase