[Liver regeneration and the immune system. 1. In vitro and in vivo activation of lymphocytes by liver regeneration].
When syngeneic lymphocytes and mitomycin C (MMC) treated regenerating liver cells prepared from a partial hepatectomized mice are cultured together, the in vitro DNA synthetic response is activated (sMLHLR: syngeneic mixed hepatectomized liver cell-lymphocyte culture). Ia+ Kupffer cells play an important role as stimulators in the responses, since the stimulating activity of regenerating liver cells is lost by the pretreatment of them with anti-Ia monoclonal antibody plus complement or the removing Kupffer cells from them. The lymphocytes are activated also in vivo during liver regeneration after a partial hepatectomy. Because, when lymphocytes prepared from hepatectomized mice are cultured with regenerating liver cells, lymphocytes are stimulated to accelerate their DNA synthesis in a typical manner of the secondary immune responses (secondary sMLHLR). In primary sMLHLR, the responder cells are mainly Lyt-1+ whereas in secondary sMLHLR they are mainly Lyt-2+. The mechanism of changing the Lyt phenotype of major responder cells from Lyt-1 to Lyt-2 during sMLHLR is discussed.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004247:DNA / Q000096:biosynthesis; D005260:Female; D008115:Liver Regeneration; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D008937:Mitomycins; D016685:Mitomycin; D004247:DNA