Application of an enzyme-linked immunosorbent assay for detection of Brucella antigens in vaginal discharge of cows.
An enzyme-linked immunosorbent assay (ELISA) was developed for detecting Brucella abortus. Carbodiimide cyanamide was used as an antigen-coupling agent and NH4Cl was used to neutralize residual active charges present on the cuvette surface. Suitable ELISA reactions were observed in vaginal swabs placed in phosphate-buffered saline solution. The ELISA described may be useful under controlled laboratory conditions for detecting B abortus-infected cows shedding bacteria in vaginal secretions.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D002004:Brucella Vaccine / Q000276:immunology; D002003:Brucella abortus / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000175:diagnosis* / Q000517:prevention & control; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007973:Leukorrhea / Q000382:microbiology / Q000662:veterinary; D014611:Vaccination / Q000662:veterinary; D014621:Vagina / Q000382:microbiology*
D000942:Antigens, Bacterial; D002004:Brucella Vaccine