Subcellular distribution of pinazepam and its metabolite N-desmethyldiazepam in rat liver.
The distribution in subcellular fractions of pinazepam (Pz) and its metabolite N-desmethyldiazepam (N-Dz) was studied after a single oral administration of Pz (20 mg/kg body wt) to rats. Animals were killed 2 min and 6 hr after dosing. The amounts of the parent drug and its metabolite retrieved in the microsomal fraction were small, but significantly higher than those in the nuclear, mitochondrial and soluble fractions. The cytosol contained the lowest percentages of both drugs. No substantial difference was found in the subcellular distribution pattern of both Pz and N-Dz 2 min and 6 hr after administration.
D000818:Animals; D014151:Anti-Anxiety Agents; D001569:Benzodiazepines; D001570:Benzodiazepinones / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D003975:Diazepam / Q000031:analogs & derivatives*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D003708:Nordazepam / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000378:metabolism
D014151:Anti-Anxiety Agents; D001570:Benzodiazepinones; D001569:Benzodiazepines; C010763:pinazepam; D003708:Nordazepam; D003975:Diazepam