Priming against stimulator-derived alloantigens bound onto MLC-activated blasts.
Several investigators have reported that antigen transfer between stimulator and responder cells occurs. We investigated whether it was possible to prime against such stimulator-derived alloantigens bound onto mixed lymphocyte culture (MLC)-activated blasts. For this purpose we generated blasts bearing bound stimulator-derived alloantigens in a primary MLC. Subsequently we primed a new family member against these blasts and after 10 days restimulated with all family members. Our results showed that it was indeed possible to sensitize against this transferred antigen and to obtain specific restimulation. Moreover we demonstrated that it was possible to abolish this specific response by treating these blast cells with trypsin.
D002478:Cells, Cultured; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D007519:Isoantigens / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D014357:Trypsin / Q000494:pharmacology
D000949:Histocompatibility Antigens Class II; D007519:Isoantigens; D014357:Trypsin