Biological and immunological characterization of purified follicle-stimulating hormone preparations from monkey pituitaries.
The suitability of a purified rhesus (rhFSH) and cynomolgus (cynFSH) follicle-stimulating hormone (FSH) preparation to replace the expired LER 1909-2 reference standard in the immunoassay of monkey FSH was assessed using an in vitro bioassay and two heterologous radioimmunoassay (RIA) procedures in combination with fractionation by high-resolution isoelectrofocusing. The results presented indicate that rhFSH is unsuitable for the replacement of LER 1909-2 as a standard, though cynFSH might be suitable after the removal of its LH contamination.
D000818:Animals; D001681:Biological Assay; D005640:Follicle Stimulating Hormone / Q000032:analysis* / Q000276:immunology / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007986:Luteinizing Hormone / Q000032:analysis; D008252:Macaca fascicularis; D008253:Macaca mulatta; D011863:Radioimmunoassay / Q000662:veterinary; D013045:Species Specificity
D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone