Suppression of antibody and T cell proliferative responses to L-glutamic acid60-L-alanine30-L-tyrosine10 by a specific monoclonal T cell factor.
The synthetic terpolymer L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) stimulates GAT-specific suppressor T cells in nonresponder mice. Extracts from these T cells contain a GAT-specific soluble T cell suppressor factor (GAT-TsF) that inhibits development of GAT-specific plaque-forming cell (PFC) responses by spleen cells from nonresponder mice stimulated with GAT complexed to methylated bovine serum albumin (GAT-MBSA). These extracts also contain a factor that inhibits development of GAT-specific proliferative responses by GAT-MBSA-primed, nonresponder lymph node T cells. Experiments reported in this manuscript show that a hybrid T cell line, produced by fusion of the AKR thymoma, BW5147, with spleen cells that contain GAT-specific suppressor T cells, produces a constitutive GAT-specific suppresor factor that functionally and serologically resembles GAT-TsF extracted from T cells. More importantly, both GAT-specific PFC and T cell proliferative responses are inhibited by this factor.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D002455:Cell Division; D002460:Cell Line; D006183:H-2 Antigens / Q000235:genetics; D006822:Hybrid Cells; D007166:Immunosuppressive Agents / Q000302:isolation & purification*; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000276:immunology*; D011108:Polymers; D013601:T-Lymphocytes / Q000032:analysis / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000032:analysis
D006183:H-2 Antigens; D007166:Immunosuppressive Agents; D010455:Peptides; D011108:Polymers; C020749:GAT