Isolation and properties of nonspecific esterase of murine L 1210 leukemia cells.
Nonspecific esterases of mouse lymphatic leukemia cells L 1210 were isolated and partly purified by gel filtration or DEAE Sephadex ion exchange chromatography. Two forms of esterases of differing molecular weight were found. Both esterases have optimum pH 7.2 and are inhibited by serine esterase inhibitors as well as by thiol reagents. They are not influenced by chelating agent (EDTA).
D000818:Animals; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000032:analysis; D007939:Leukemia L1210 / Q000201:enzymology*; D051379:Mice; D008811:Mice, Inbred DBA; D008970:Molecular Weight; D009368:Neoplasm Transplantation
D007527:Isoenzymes; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase