Filter radioimmunoassay, a method for large-scale serotyping of Neisseria meningitidis.
A simple and rapid filter radioimmunoassay method can be used to serotype meningococcal strains on a large scale. The technique consists of simultaneous inoculation of 96 strains on nitrocellulose filters. The resulting colonies can be processed in situ, by extraction and fixation, incubation with antibodies and 125I-labeled protein A, and, finally, autoradiography. Processing many filters simultaneously, one person can serotype thousands of meningococci in a week. Multiple filters with identical strain patterns can be stored after the fixation step for future screening. The use of monoclonal antibodies is essential; polyclonal antisera, even after extensive absorption, were not specific in this assay. When results from filter radioimmunoassay and Ouchterlony microprecipitation were compared for the serotyping of 201 Neisseria meningitidis strains for serotypes 2a and 2b, filter radioimmunoassay was sufficiently sensitive and specific to be useful in mass screening.
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D005779:Immunodiffusion; D009345:Neisseria meningitidis / Q000145:classification* / Q000276:immunology; D011863:Radioimmunoassay; D012703:Serotyping / Q000379:methods*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal