Simplified urinary oxalate determination using an enzyme electrode.
An enzyme electrode for urine oxalate measurement has been produced using acrylamide gel-entrapped oxalate decarboxylase retained over a CO2 sensor. Urine required pre-treatment with EDTA, but oxalate extraction was not necessary and inhibition by phosphate and sulphate was not apparent until after 10-14 days. The linear range was 0-0.2 mmol/l. Analysis of 50 urine specimens diluted 1 in 10 in pH 3.0 glycine buffer showed good correlation with a widely-used colorimetric method (y = 1.101x-0.018, r = 0.955).
D000328:Adult; D002245:Carbon Dioxide; D002262:Carboxy-Lyases; D004492:Edetic Acid; D004566:Electrodes; D004800:Enzymes, Immobilized; D005260:Female; D006801:Humans; D008297:Male; D008722:Methods; D010070:Oxalates / Q000652:urine*; D019815:Oxalic Acid; D013997:Time Factors
D004800:Enzymes, Immobilized; D010070:Oxalates; D002245:Carbon Dioxide; D019815:Oxalic Acid; D004492:Edetic Acid; D002262:Carboxy-Lyases; C021915:oxalate decarboxylase