Gamma heavy chain disease studied by two-dimensional electrophoresis and immuno-blotting techniques.
We used two-dimensional polyacrylamide gel electrophoresis and immunoblotting techniques to study serum proteins from a patient with a monoclonal gammopathy. Two-dimensional electrophoresis was optimized for serum proteins with two main goals: (a) to allow the resolution of many serum proteins in both directions, with penetration of the maximum number of proteins in the first dimension; and (b) to obtain the best reproducibility from one experiment to another, within the limits of the current technique. These analyses, combined with immunoblotting, permitted us to characterize a gamma heavy chain disease protein of 34 000-Da molecular mass. Moreover, two-dimensional mapping of the patient's serum proteins allowed demonstration of the microheterogeneity of this monoclonal component.
D001798:Blood Proteins / Q000032:analysis*; D004586:Electrophoresis; D004588:Electrophoresis, Cellulose Acetate; D004591:Electrophoresis, Polyacrylamide Gel; D006362:Heavy Chain Disease / Q000097:blood*; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007124:Immunoenzyme Techniques; D007143:Immunoglobulin Heavy Chains / Q000032:analysis*; D007142:Immunoglobulin gamma-Chains / Q000032:analysis*; D007525:Isoelectric Focusing; D008970:Molecular Weight
D001798:Blood Proteins; D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains