Adjuvant requirements for protective immunization of mice using a Trypanosoma cruzi 90K cell surface glycoprotein.
A wide range of adjuvants including alhydrogel, saponin, Corynebacterium parvum, DDAB, Pfizer CP-20,961, oil adjuvants and several MDP analogues have been compared for their adjuvant activity in protecting mice against lethal Trypanosoma cruzi infection following immunization with a T. cruzi 90K cell surface glycoprotein. Only saponin was found to be effective. Promotion did not correlate with the ability to promote a particular Ig isotype; however, saponin was unique in its ability to promote cell-mediate immunity against the 90K glycoprotein.
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000008:administration & dosage; D000276:Adjuvants, Immunologic; D000818:Animals; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology; D014355:Chagas Disease / Q000517:prevention & control*; D006023:Glycoproteins / Q000008:administration & dosage*; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007114:Immunization; D051379:Mice; D008808:Mice, Inbred CBA; D011425:Propionibacterium acnes / Q000276:immunology; D000644:Quaternary Ammonium Compounds / Q000008:administration & dosage; D012503:Saponins / Q000008:administration & dosage; D013185:Squalene / Q000008:administration & dosage; D014349:Trypanosoma cruzi / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000954:Antigens, Surface; D006023:Glycoproteins; D000644:Quaternary Ammonium Compounds; D012503:Saponins; C015831:dimethyldioctadecylammonium; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D013185:Squalene