Effect of 5-fluoro-2'-deoxyuridine on deoxyribonucleotide pools in vivo.
5-Fluoro-2'-deoxyuridine (FdUrd) lowered the dTTP levels in rapidly frozen 12-day W rat embryos and in a human neuroblastoma grown in nude N:NIH(S) mice to about 20% of control values. This effect was associated with greatly increased dCTP levels and reduction of dGTP levels essentially to zero. Elimination of the dGTP pool correlated temporally with the cytotoxicity of FdUrd. Extremely rapid fixation of tissue was required to avoid artifactually high deoxyribonucleoside triphosphate values.
D000818:Animals; D046508:Culture Techniques / Q000379:methods; D003854:Deoxyribonucleotides / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D005467:Floxuridine / Q000494:pharmacology*; D006801:Humans; D008431:Maternal-Fetal Exchange; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats
D003854:Deoxyribonucleotides; D005467:Floxuridine