title: High spontaneous thymidine incorporation into a non-T lymphocyte population in Hodgkin's disease unmasked after cell fractionation.
abstract: In order to study the nature of the spontaneously stimulated cells in Hodgkin's disease, lymphocytes from 10 patients with Hodgkin's disease and 10 healthy control subjects were separated into low- and high-density fractions. Both fractions were subdivided into T and non T cell populations by means of rosette sedimentation. In Hodgkin's disease patients, non-T cells with a high density showed a significantly increased spontaneous thymidine incorporation (mean, 5677 cpm; range, 405--26490 cpm) compared to corresponding cells from control subjects (mean, 484 cpm; range, 235--850 cpm), and to unseparated high-density lymphocytes in Hodgkin's disease patients. Evidence is presented that in Hodgkin's disease, the high-density lymphocyte fraction comprises non-T cells with an apparent potency for DNA synthesis which is, however, inhibited by T cells present in the same high-density fraction.
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000378:metabolism; D002469:Cell Separation; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005260:Female; D006689:Hodgkin Disease / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism*
chemical_list: D004273:DNA, Neoplasm; D013936:Thymidine