Phenotypic expression of B-lymphocytes. 1. Identification with monoclonal antibodies in normal lymphoid tissues.
Marker expression is highly variable among different stages of B-cell activation. In peripheral lymphoid tissues, architecturally and cytologically three or four types of B cells can be identified, thus allowing an investigation of cellular surface or cytoplasmic phenotypes. Mantle zone lymphocytes of follicles are phenotypically similar, if not identical, to peripheral blood B-lymphocytes and express the following markers: B1, B2, BA-1, HLA-DR, Leu 10, A1G3, Leu 8, and T200. The germinal center is composed of two main populations, centroblasts and centrocytes which express the following markers: B1, BA-2, J5, OKT9, HLA-DR, Leu 10, and T200. This phenotype is similar to that of activated B cells or pre-B cells. Although membranous OKT10 is present in cortical thymocytes, the presence of cytoplasmic OKT10 appears to be a useful marker for plasma cells, terminally differentiated B cells, which also express A1G3 and T200. This study also revealed that a subpopulation of T cells in the germinal center express different markers (A1G3-/Leu 8-) as compared with that of most T cells in the T-dependent zone (A1G3+/Leu 8+).
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D005802:Genes, MHC Class II; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000276:immunology; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D008221:Lymphoid Tissue / Q000166:cytology* / Q000276:immunology; D051379:Mice; D010641:Phenotype
D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D000954:Antigens, Surface; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II