Monoclonal antibodies to murine immunoglobulin isotypes.
We have obtained cell lines producing monoclonal antibodies against mu, alpha, lambda, and lambda 1 by fusing the mouse myeloma P3X63Ag8.653 with spleen cells from Lewis rats immunized with several lambda 1 bearing myeloma proteins. The anti-lambda 1 antibody is particularly useful. It was shown to be suitable for radioimmunoassays, for ELISAs when coupled to alkaline phosphatase, and for indirect fluorescence staining of the membrane as well as the cytoplasm.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007126:Immunoglobulin Allotypes / Q000276:immunology*; D007137:Immunoglobulin alpha-Chains / Q000276:immunology; D007146:Immunoglobulin lambda-Chains / Q000276:immunology; D007148:Immunoglobulin mu-Chains / Q000276:immunology; D051379:Mice; D051381:Rats
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007126:Immunoglobulin Allotypes; D007137:Immunoglobulin alpha-Chains; D007146:Immunoglobulin lambda-Chains; D007148:Immunoglobulin mu-Chains