Evidence for the occurrence of a fast-acting inhibitor for tissue-type plasminogen activator in human plasma.
Human plasma contains a fast-acting t-PA inhibitor, which is not identical with alpha 2-antiplasmin or alpha 2-macroglobulin. The concentration of this inhibitor in normal plasma is highly variable, much lower than that of known plasma protease inhibitors, and in the range of physiologically occurring plasma concentrations of t-PA (0-2 IU/ml). The inhibitor binds to concanavalin A-Sepharose, is rather stable when heated, is not precipitated in euglobulin fractions and probably does not originate from platelets. The inhibitor seems to form a 190 Kd complex with t-PA. The relation between this plasma inhibitor and the recently discovered endothelial cell inhibitor is not yet clear.
D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification*; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D008970:Molecular Weight; D010960:Plasminogen Activators / Q000037:antagonists & inhibitors*; D015849:Plasminogen Inactivators
D006023:Glycoproteins; D007457:Iodine Radioisotopes; D015849:Plasminogen Inactivators; D010960:Plasminogen Activators