[Method of isolating and controlling fluorescent Fab fragments of antibodies against horse serum proteins].
For the first time the lyophilized fluorescent Fab-fragments of rabbit antibodies to horse serum protein, suitable for detecting different antigens and antibodies to these antigens, have been obtained by the specially developed method. The criteria to be used in the control of the antispecific fluorescent fragments of antibodies have been described and the methods of their control before and after lyophilization have been developed. The use of the antispecific fluorescent Fab-fragments of antibodies has been shown to considerably accelerate and simplify the indirect immunofluorescent assay.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D001798:Blood Proteins / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D005612:Freeze Drying; D006736:Horses / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000032:analysis / Q000302:isolation & purification*; D011817:Rabbits; D012283:Rickettsia prowazekii / Q000276:immunology
D000907:Antibodies, Bacterial; D001798:Blood Proteins; D007140:Immunoglobulin Fab Fragments
Metodika polucheniia i kontrolia fluorestsiruiushchikh Fab-fragmentov antitel protiv belkov syvorotki loshadi.