Suppression of pokeweed mitogen-induced differentiation of human B cells by in vitro levamisole-treated T lymphocytes.
The generation of immunoglobulin-producing cells from human peripheral blood lymphocytes in pokeweed mitogen-simulated cultures was significantly suppressed by levamisole. Greatest suppression was observed when cells were incubated with levamisole at a concentration of 10(-6) M for 20 min or more prior to culture. Such suppression occurred when untreated as well as levamisole-treated B cells were co-cultured only with levamisole-treated T cells. Adding of levamisole-treated T cells to a combination of untreated autologous B and T cells resulted in the suppression of B cell differentiation. The results suggest that levamisole treatment of lymphocytes exerts suppression on pokeweed mitogen-induced B cell differentiation through its effect on T cell regulatory properties.
D000328:Adult; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007978:Levamisole / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects; D011043:Pokeweed Mitogens / Q000494:pharmacology; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology
D011043:Pokeweed Mitogens; D007978:Levamisole