Functional expression of a cloned I-A beta k gene in B-lymphoma cells.
The immune response genes of the mouse encode two cell-surface glycoproteins, I-A and I-E, that play critical roles in determining the animal's immune responsiveness. The I-A antigen contains two chains, alpha and beta. A cloned beta-chain gene, I-A beta k, was introduced into B-lymphoma cells that express I-Ad. The transfected gene was successfully expressed on the cell surface of the recipient cells and was functional in stimulating allospecific T cells.
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005802:Genes, MHC Class II; D006579:Heterozygote; D008211:Lymphocyte Cooperation; D008223:Lymphoma; D046911:Macromolecular Substances; D051379:Mice; D013601:T-Lymphocytes / Q000502:physiology; D014162:Transfection; D014170:Transformation, Genetic
D046911:Macromolecular Substances