Extraction from bone powder: collagen, calcium and phosphate.
A method for the estimation of the extraction rate is shown and exemplified by preliminary data from extraction of collagen, calcium and phosphate with EDTA and neutral salt solution. The initial extraction rate was high, even with neutral salt solution, and optimal extraction efficiency was obtained within 24 h.
D001842:Bone and Bones / Q000032:analysis*; D002118:Calcium / Q000302:isolation & purification*; D003094:Collagen / Q000302:isolation & purification*; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D008722:Methods; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D010710:Phosphates / Q000302:isolation & purification*; D013054:Spectrophotometry, Atomic
D010710:Phosphates; D010664:Phenylmethylsulfonyl Fluoride; D003094:Collagen; D004492:Edetic Acid; D002118:Calcium