Picogram detection of eicosanoids by ultraviolet absorbance after narrow-bore high-performance liquid chromatography. Comparison with conventional-bore column.
Mobile-phase variations were employed to achieve optimal separation by narrow-bore reversed-phase high-performance liquid chromatography of eleven eicosanoids. Separation and quantitation by ultraviolet absorbance at 190 nm using conventional-bore ODS columns were compared. Using the improved sensitivity obtained by means of the narrow-bore column, i.e. 250-pg detection limits of a standard solution, analysis of eicosanoids in kidney medulla was achieved. Parallel quantitation by radioactivity, using [1-(14)C]arachidonic acid as substrate, was applied.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D007679:Kidney Medulla / Q000032:analysis; D011453:Prostaglandins / Q000032:analysis*; D011465:Prostaglandins, Synthetic / Q000032:analysis*; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D001095:Arachidonic Acids; D011453:Prostaglandins; D011465:Prostaglandins, Synthetic; D016718:Arachidonic Acid