Metabolism of dihydrouracil in Rhodosporidium toruloides.
Previous studies, including those done with a similar species, have indicated that dihydrouracil is formed by the breakdown of uracil and is degraded into N-carbamyl-beta-alanine. (Fink et al., J. Biol. Chem. 201:349-355, 1953; S. R. Vilks and M. Y. Vitols, Mikrobiologiya 42:567-583, 1973; O. A. Milstein and M. L. Bekker, J. Bacteriol. 127:1-6, 1976). In the present work the conversion of dihydrouracil to uracil is studied in Rhodosporidium toruloides, and the growth characteristics of mutants that have lost the ability to use dihydrouracil as a source of nitrogen are examined. It is concluded that dihydrouracil must be converted to uracil before catabolism of the pyrimidine ring can take place.
D001487:Basidiomycota / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D009154:Mutation; D010088:Oxidoreductases / Q000378:metabolism; D044925:Oxidoreductases Acting on CH-CH Group Donors; D014498:Uracil / Q000031:analogs & derivatives* / Q000378:metabolism; D014542:Uridine Monophosphate / Q000378:metabolism; D014587:Ustilaginales / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
C007419:dihydrouracil; D002245:Carbon Dioxide; D014498:Uracil; D014542:Uridine Monophosphate; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C041154:dihydrouracil oxidase