[Rate of elimination of human high-density lipoproteins after their intravenous administration in rabbits by the loss of alpha- cholesterol and apoprotein A-1].
Native HDL, HDL2 or HDL3, isolated from donor plasma were intravenously administered at a large dose (100 mg HDL cholesterol) to healthy rabbits and rabbits with experimental hypercholesterolemia. The elimination rate of human HDL was studied on the basis of alpha-cholesterol (alpha-Ch) and apoprotein (apo) A-I determinations in the plasma of rabbits. Administration of HDL or their subclasses at a large dose with the following determination of alpha-Ch or apo A-I enabled to avoid the use of radioactive label and all the methodological errors connected with the latter. After administration of HDL, HDL2 and HDL3 their elimination according to alpha-Ch determination occurred at the higher rate as compared with apo A-I. Elimination of the total HDL fraction according to alpha-Ch determination was higher in healthy rabbits than in animals with hypercholesterolemia while the elimination estimated by the apo A-I determination was similar in both groups of animals. These data suggest that HDL may characterize the Ch-donor properties. Apo A-I as compared with alpha-Ch was a more accurate indicator of HDL concentration in blood plasma.
D000818:Animals; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000097:blood*; D002784:Cholesterol / Q000097:blood*; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood*; D007275:Injections, Intravenous; D007700:Kinetics; D008075:Lipoproteins, HDL / Q000008:administration & dosage / Q000097:blood*; D053439:Lipoproteins, HDL2; D053440:Lipoproteins, HDL3; D008297:Male; D011817:Rabbits
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D008075:Lipoproteins, HDL; D053439:Lipoproteins, HDL2; D053440:Lipoproteins, HDL3; D002784:Cholesterol
Izuchenie skorosti éliminatsii chelovecheskikh lipoproteidov vysokoĭ plotnosti posle ikh vnutrivennogo vvedeniia krolikam po ubyli al'fa-kholesterina i apoproteina A-1.