title: Different tissue distributions of two types of thiol proteinase inhibitors from rat liver and epidermis.
abstract: The concentrations of two types of endogenous inhibitors of thiol proteinases were determined in soluble extracts of various rat tissues by means of a sensitive enzyme immunoassay method, which consisted of solid-phase immobilized anti-rat liver inhibitor or anti-rat epidermal inhibitor and antibodies labeled with horseradish peroxidase. The minimum detectable amounts of inhibitors from liver and epidermis were 30 pg and 3 pg/assay, respectively. The tissue distributions of the epidermal and liver-type inhibitors were found to differ. The liver-type inhibitor was found to be widely distributed in various tissues at levels of 76-420 ng/mg protein, whereas the epidermal-type inhibitor was found at high levels in the skin, tongue, esophagus, stomach, intestine, and vagina, but at quite low levels in other tissues tested.
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004817:Epidermis / Q000737:chemistry*; D005260:Female; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D008099:Liver / Q000737:chemistry*; D008297:Male; D011480:Protease Inhibitors / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D011480:Protease Inhibitors