Use of a simple in vivo technique to investigate components of the immediate hypersensitivity reaction in conscious guinea pigs.
A semiplethysmographic method was used to delineate the effects of aerosol antigen challenge on the respiratory pattern of conscious, sensitized guinea pigs. Chlorpheniramine pretreatment prevented death, delayed the onset of respiratory symptoms and decreased the severity of the hypersensitivity reaction. This reaction could be further diminished to varying degrees by the addition of FPL55712, atropine sulfate and/or imidazole to chlorpheniramine. Indomethacin and nordihydroguaiaretic acid (NDGA) with chlorpheniramine caused a potentiation in the severity of the hypersensitivity reaction. However, chlorpheniramine, NDGA and imidazole in combination blocked the hypersensitivity reaction. The technique appears to demonstrate the involvement of several suggested mediators in asthma and appears potentially useful as an indicator of effects on arachidonic acid metabolism and metabolites.
D000336:Aerosols; D000707:Anaphylaxis / Q000188:drug therapy; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001986:Bronchial Spasm / Q000188:drug therapy; D002744:Chlorpheniramine / Q000494:pharmacology*; D002867:Chromones / Q000494:pharmacology; D006168:Guinea Pigs; D006969:Hypersensitivity, Immediate / Q000503:physiopathology*; D007213:Indomethacin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008084:Lipoxygenase / Q000378:metabolism; D008297:Male; D011292:Premedication; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism
D000336:Aerosols; D002867:Chromones; D002744:Chlorpheniramine; D001285:Atropine; C006312:FPL 55712; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases; D007213:Indomethacin