Epidermal Langerhans cells contain intermediate-sized filaments of the vimentin type: an immunocytologic study.
In epidermal cell suspensions, prepared from healthy human and murine skin, Langerhans cells (LC) were identified by indirect immunofluorescence microscopy using monoclonal antibodies directed against human T6 and Ia markers, and against a murine Iak determinant, respectively. The cells were double-labeled on cytospin slides with antibodies against a cytoskeletal protein, either vimentin or keratin. In this way it was shown that epidermal LC contain intermediate-sized filaments of the vimentin type.
D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000276:immunology; D005455:Fluorescent Antibody Technique; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007381:Intermediate Filament Proteins / Q000032:analysis*; D007801:Langerhans Cells / Q000032:analysis*; D051379:Mice; D011817:Rabbits; D014746:Vimentin
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D007381:Intermediate Filament Proteins; D014746:Vimentin