Reactivation of serum creatine kinase isoenzyme BB in patients with malignancies.
Reactivation of serum creatine kinase isoenzyme BB (CK-BB) with 2-mercaptoethanol and EDTA increased the electrophoretic detection rate of CK-BB from 34% to 78% in 58 hospitalized patients with various malignancies. Patients with solid tumors showed the largest and patients with hematologic malignancies the smallest percentage increase in CK-BB after reactivation. For serum from 50 hospitalized patients without cancer, reactivation resulted in detectable CK-BB in two patients; the CK-BB band was never seen in 15 healthy adults. For reasons unknown, five of eight patients with senile dementia of the Alzheimer's type showed CK-BB in serum after reactivation, as did two of five patients suspected of having this disorder. Serum CK-BB may be a useful tumor marker if reactivation with a thiol and EDTA is used immediately after collection.
D000263:Adenylate Kinase / Q000097:blood; D000544:Alzheimer Disease / Q000201:enzymology; D001797:Blood Protein Electrophoresis; D002614:Chelating Agents / Q000494:pharmacology*; D003402:Creatine Kinase / Q000097:blood*; D004492:Edetic Acid / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D004793:Enzyme Reactivators; D006801:Humans; D007527:Isoenzymes; D008623:Mercaptoethanol / Q000494:pharmacology; D009369:Neoplasms / Q000201:enzymology*; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
D002614:Chelating Agents; D004793:Enzyme Reactivators; D007527:Isoenzymes; D013438:Sulfhydryl Compounds; D008623:Mercaptoethanol; D004492:Edetic Acid; D003402:Creatine Kinase; D000263:Adenylate Kinase