title: Antigen-specific induction and regulation of antibody synthesis in cultures of human peripheral blood mononuclear cells.
abstract: Culture conditions suitable for specific sensitization of human peripheral blood lymphocytes were established. Production of antibody to sheep erythrocytes and to the 2,4,6-trinitrophenyl group conjugated to burro erythrocytes was investigated in terms of cross-sensitization, feedback control by antibody to the immunogen, and regulation by helper and suppressor T cells. Generation of antigen-specific antibody-forming cells was found to be restricted to a narrow concentration range of cultured peripheral blood lymphocytes. The data suggest that antibody formation is limited by suppressor T cells in high-cell-density cultures and by low frequencies of helper T cells in low-cell-density cultures.
mesh_terms: D000917:Antibody Formation / Q000187:drug effects; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000276:immunology*; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D006801:Humans; D008211:Lymphocyte Cooperation; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D000941:Antigens; D003470:Culture Media; D003208:Concanavalin A