title: [Luria effect in bacteriophages and the role of the products of recA+ and lexA+ genes in its induction].
abstract: An analysis of UV-damages accumulation in the phages as revealed by delay of intracellular growth is represented using temperate lambda phage. The maximum of growth delay of phage lambda at given UV-dose was found with lambda red+, infecting Escherichia coli AB1886 uvrA strain. The growth delay was absent, when a strain RH-1 uvrA-recA- was infected with UV-irradiated phage lambda red3. A moderate growth delay was obtained with the phages lambda red+, infecting E. coli RH-1 uvrA-recA- or phage lambda red3, infecting E. coli AB1886 uvrA-. THe growth delay was also absent when wild type, recA- and uvrA mutants of E. coli were infected with phage lambda after 8-metnoxypsoralen + light (lambda > 310 nm) treatment. It is known that the crosslinks appear to be the DNA defects which give rise to the observed biological inactivation following psoralen + light treatment. However, a considerable growth delay of phage lambda, treated by 8-metnoxypsoralen + light, was only found under condition of crosslinks repair (W-reactivation and prophage-reactivation). The results obtained are best explained by the assumption that the growth delay reflects the time required for the postreplication repair (RecA, LexA, Red) of any lethal UV-lesion.
mesh_terms: D001426:Bacterial Proteins / Q000502:physiology*; D010582:Bacteriophage lambda / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects*; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics*; D005814:Genes, Viral; D008027:Light; D008730:Methoxsalen / Q000494:pharmacology; D014466:Ultraviolet Rays; D014779:Virus Replication
chemical_list: D001426:Bacterial Proteins; D008730:Methoxsalen
vernacular_title: Effekt Luria u bakteriofagov i rol' produktov genov recA+ i lexA+ v ego induktsii.