Reversed phase high-performance liquid chromatography of heterocyclic aromatic N-oxides: application to measurement of N-oxidation by microsomes and rat hepatocytes in primary culture.
1. The rates of N-oxide formation from pyridine, nicotinamide and isonicotinamide by rat liver microsomes and by cultured rat hepatocytes have been quantified using a simple h.p.l.c. system. 2. Hepatocytes produce hetero-aromatic N-oxides for at least 5 h, making them a useful system to study the N-oxidation of pyridines.
D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003497:Cyclic N-Oxides / Q000302:isolation & purification*; D008099:Liver / Q000166:cytology; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D009536:Niacinamide / Q000378:metabolism; D010084:Oxidation-Reduction; D011725:Pyridines / Q000378:metabolism; D051381:Rats
D003497:Cyclic N-Oxides; D011725:Pyridines; D009536:Niacinamide; C027681:isonicotinamide