The Ia-1 gene effects T-B cell collaboration for the in vitro antibody response.
The I-A subregion mutant B6.C-H-2bm12 (bm12) and the C57BL/6 parent were used to examine the role of the Ia-1 gene product as a restriction element in the antibody response to sheep red blood cells (SRBC). Using an in vitro culture system, it was shown that cooperation between histocompatible T cells, B cells and macrophages was required to generate a secondary IgM response to SRBC. The alteration in the Ia-1 gene in bm12 altered the ability of bm12 T cells to collaborate with C57BL/6 B cells, but not with C57BL/6 macrophages. Similarly, C57BL/6 T cells could not collaborate with bm12 B cells. The mutation in bm 12 did not affect the ability of T cells to interact with C57Bl/6 macrophages, either in vitro or in vivo, or of bm12 macrophages to interact with C57BL/6T cells. Thus in this system, the Ia-1 gene product restricts T-B cell interaction but not T cell-macrophage interaction.
D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D002450:Cell Communication; D004912:Erythrocytes / Q000276:immunology; D005802:Genes, MHC Class II; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis; D008285:Major Histocompatibility Complex; D051379:Mice; D008817:Mice, Mutant Strains; D013601:T-Lymphocytes / Q000276:immunology*
D007075:Immunoglobulin M