[Changes in the cytoskeleton and surface charge as a possible cause of the increased mechanical binding between enterocytes in the small intestine epithelium induced by 1,2-dimethylhydrazine].
Alterations in the mechanical linkage between the enterocytes in BALB/c mouse colonic epithelium were investigated under the influence of agents affinitable to the cell surface and cytoskeleton. It is found that cytochalasin B (10(-6) - 10(-5) g/ml), low pH (pH 3) and low concentration of colchicine (10(-8) g/ml) increased, but EDTA (5 X 10(-3) g/ml) and high concentration of colchicine (10(-4) g/ml) reduced the magnitude of mechanical linkage between the enterocytes. A comparative analysis of the data obtained with the control and with 1,2-dimethylhydrazine (DMH) treated mice indicates that probable reasons of a DMH-induced rise in the intercellular mechanical linkage may involve the decrease in electrostatic repulsive forces between the cell surfaces, and the weakening of centripetal tensions due to a decrease in negative surface charge and disorganization of the microfilament system, respectively. These alterations of the cell surface negative charge and the cytoskeleton are expressed on day 3 after a single injection of DMH and are seen to persist during repeated carcinogen applications.
D019813:1,2-Dimethylhydrazine; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects* / Q000648:ultrastructure; D004127:Dimethylhydrazines / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000648:ultrastructure; D005260:Female; D006863:Hydrogen-Ion Concentration; D007421:Intestine, Small / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008763:Methylhydrazines / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013499:Surface Properties
D002273:Carcinogens; D004127:Dimethylhydrazines; D008763:Methylhydrazines; D003571:Cytochalasin B; D004492:Edetic Acid; D019813:1,2-Dimethylhydrazine; D003078:Colchicine
Izmeneniia tsitoskeleta i zariada poverkhnosti kak vozmozhnaia prichina povsyheniia mekhanicheskoĭ sviazi mezhdu énterotsitami v épiteliĭ tolstoĭ kishki, indutsirovannogo 1,2-dimetilgidrazinom.