Immunological detection of cell surface galactosyltransferase in preimplantation mouse embryos.
Presence of cell surface galactosyltransferase was surveyed in preimplantation mouse embryos by indirect immunofluorescence staining using an affinity-purified antibody against galactosyltransferase from human milk. Distinct fluorescence staining was observed in embryos ranging from late 8-cell stage to early blastocysts, while the embryos at other stages were stained only weakly. The cell surface enzyme was also present in F9 embryonal carcinoma cells, in a fraction of bone marrow cells of the mouse, and in a few percent of testicular sperm.
D000818:Animals; D001755:Blastocyst / Q000201:enzymology*; D001757:Blastomeres / Q000201:enzymology; D001853:Bone Marrow / Q000201:enzymology; D002462:Cell Membrane / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D005700:Galactosyltransferases / Q000032:analysis*; D006651:Histocytochemistry; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008813:Mice, Inbred ICR; D008895:Milk, Human / Q000201:enzymology; D009028:Morula / Q000201:enzymology; D013094:Spermatozoa / Q000201:enzymology; D013724:Teratoma / Q000201:enzymology
D005700:Galactosyltransferases