Formation of leukotriene C4-like material by rat brain tissue.
Pieces of rat brain incubated in oxygenated Tyrode solution were stimulated with the divalent cation ionophore A 23187. Incubation media were assayed for leukotriene (LT) C4-like immunoreactivity as well as for prostaglandin (PG) D2, 6-keto-PGF1 alpha and thromboxane (TX) B2 using specific and sensitive radioimmunoassays. Spontaneous release of the cyclooxygenase products but only of trace amounts of LTC4-like immunoreactivity was detected. Stimulation with ionophore A 23187 increased the level of cyclooxygenase products and to a smaller extent the level of LTC4-like immunoreactivity. Inhibition of cyclooxygenase by indomethacin was paralleled by a significantly increased release of LTC4-like material under basal conditions as well as in the presence of ionophore A 23187. On the other hand, the synthesis of LTC4-like material was significantly decreased by the lipoxygenase inhibitors quercetin and nordihydroguaiaretic acid. The presence of LT-like material in rat brain incubates was confirmed by bioassay. The capacity of brain tissue to synthesize LT-like material may be important for cerebral blood flow as well as cellular functions in the central nervous system.
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D016861:Cyclooxygenase Inhibitors; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D015230:Prostaglandin D2; D011457:Prostaglandins D / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013189:SRS-A / Q000096:biosynthesis*; D013268:Stimulation, Chemical; D013929:Thromboxane B2 / Q000096:biosynthesis
D001095:Arachidonic Acids; D016861:Cyclooxygenase Inhibitors; D011457:Prostaglandins D; D013189:SRS-A; D000001:Calcimycin; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D015230:Prostaglandin D2; D007213:Indomethacin