title: Blood ionic calcium responses of hens from thick-shell and thin-shell lines to ethyleneglycol-bis-(B-aminoethylether)-N,N'-tetraacetic acid injections.
abstract: Experiments were conducted on laying and nonlaying hens from genetically selected lines that produce thick (TK) or thin (TN) eggshells. At 44 weeks of age, TK layers exhibited significantly higher total plasma calcium concentrations than TN layers. Total plasma calcium concentrations were not significantly different between TK and TN layers at 80 weeks of age, reflecting an effect of aging on total plasma calcium. Intravenous injections of ethyleneglycol-bis-(B-aminoethylether-N,N'-tetraacetic acid (EGTA) were administered to TK and TN layers at 44 weeks of age and to TK and TN layers and nonlayers at 80 weeks of age. EGTA is a calcium chelating agent that creates hypocalcemia when injected intravenously. Laying hens of both lines and at both ages exhibited a similar degree of hypocalcemia during EGTA injections, and a similar rate of recovery to normocalcemia was noted after cessation of EGTA administration. Nonlayers were unable to recover from the EGTA-induced hypocalcemia. Because the pattern of calcium depression and recovery during and after the EGTA challenge has been shown to be associated with the capacity of parathyroid hormone-sensitive target tissues to buffer blood ionized calcium, it was concluded that the capacity to buffer ionized calcium by these target tissues is similar in TK and TN layers. However, nonlayers have a restricted capacity to buffer blood ionic calcium.
mesh_terms: D000818:Animals; D002118:Calcium / Q000097:blood*; D002645:Chickens / Q000235:genetics / Q000378:metabolism*; D003864:Depression, Chemical; D004528:Egg Shell / Q000032:analysis; D004533:Egtazic Acid / Q000494:pharmacology*; D005026:Ethylene Glycols / Q000494:pharmacology*; D005260:Female
chemical_list: D005026:Ethylene Glycols; D004533:Egtazic Acid; D002118:Calcium