The effect of fixation on esterases.
The localization of reaction product for non-specific esterase from fresh and aldehyde treated glandular tissue was examined. The electrophoretical studies showed a selective inhibition of certain isoenzymes and a change in mobility of some bands caused by aldehyde fixation. In sections a granular reaction could be identified after 5 min fixation with 1% formaldehyde while no such staining was visible in fresh tissue.
D000818:Animals; D004950:Esterases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005404:Fixatives / Q000494:pharmacology*; D005557:Formaldehyde / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D006651:Histocytochemistry; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013363:Submandibular Gland / Q000201:enzymology
D005404:Fixatives; D005557:Formaldehyde; D004950:Esterases; D005976:Glutaral