Inhibitory substances in horse sera used in the preparation of microbiological culture media.
The failure of N. gonorrhoeae to grow on isolation media was found to be due to inhibitory substances present in commercially available horse sera. Subsequent investigations indicated that the inhibitory action of the horse serum may have been due to antibodies to N. gonorrhoeae, H. influenzae, H. parainfluenzae and beta hemolytic streptococci. This experience highlights the need for media quality control programmes in laboratories which prepare microbiological culture media.
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D003470:Culture Media / Q000592:standards*; D006736:Horses / Q000276:immunology*; D009344:Neisseria gonorrhoeae / Q000254:growth & development; D011786:Quality Control
D000907:Antibodies, Bacterial; D003470:Culture Media