Quantitative semi-automated enzymatic assay for tissue glycogen.
A simple, rapid, specific and reproducible assay for tissue glycogen is described. The method involves the incubation of a denatured tissue homogenate with amyloglucosidase resulting in the complete hydrolysis of glycogen to glucose. The glucose content of the homogenate supernatant is then determined with the aid of a glucose analyzer. The method has been used to measure the glycogen content of a variety of discrete and complex animal tissues and is particularly convenient for microdeterminations.
D000818:Animals; D001322:Autoanalysis / Q000295:instrumentation; D005399:Fishes; D005087:Glucan 1,4-alpha-Glucosidase; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase; D006003:Glycogen / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008974:Mollusca; D011077:Polychaeta; D011817:Rabbits; D013696:Temperature
D006003:Glycogen; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005087:Glucan 1,4-alpha-Glucosidase; D005947:Glucose