Cysteine: a potential source of error in amino acid analysis of mercaptoethane sulfonic or hydrochloric acid hydrolysates of proteins and peptides.
Hydrolysis of proteins and peptides with mercaptoethane sulfonic acid is liable to produce overestimation of the proline content owing to the production of ninhydrin-positive material (probably cysteine) which coelutes with proline on many ion-exchange analytical systems. A similar error occurs with HCl hydrolysis (especially in the presence of mercaptoethanol or thioglycollic acid) if care is not taken to oxidize cysteine during reconstitution of the hydrolysate before amino acid analysis.
D000596:Amino Acids / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D003545:Cysteine / Q000032:analysis*; D005978:Glutathione; D006851:Hydrochloric Acid; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007474:Ion Exchange; D015080:Mesna; D010455:Peptides; D011392:Proline / Q000032:analysis; D011506:Proteins
D000596:Amino Acids; D010455:Peptides; D011506:Proteins; D011392:Proline; D005978:Glutathione; D003545:Cysteine; D015080:Mesna; D006851:Hydrochloric Acid