Accounting for the ATP-consuming processes in rabbit reticulocytes.
The report deals with a detailed balance of ATP production and consumption of the rabbit reticulocyte. The sum-total of ATP produced amounts to 135 mmol . 1-1 . h-1. About 70% of the ATP consumption has been accounted for by specific processes. The main contributing processes are the globin synthesis with about 28%, the Na+, K+-ATPase with 23% and the proteolysis with more than 15%. 30% of ATP consumption has not been accounted for. Cycloheximide (20 microM) leads to a dissociation between synthesis and degradation of proteins, which argues against any obligatory connection between these processes. More than 90% of the lysine liberated from mitochondria by proteolysis were reutilized for the globin synthesis demonstrating the high nitrogen economy of reticulocytes. Each of the ATP-consuming processes studied appears to control ATP production in an independent manner without competition with each other.
D000255:Adenosine Triphosphate / Q000097:blood*; D000818:Animals; D005914:Globins / Q000096:biosynthesis; D066298:In Vitro Techniques; D008239:Lysine / Q000097:blood; D010101:Oxygen Consumption; D010710:Phosphates / Q000097:blood; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism*
D010710:Phosphates; D000255:Adenosine Triphosphate; D005914:Globins; D008239:Lysine