Imino acid transport across the brush-border membrane of the guinea-pig small intestine.
The transport of imino and non-alpha-amino acids across the brush-border membrane of the guinea-pig small intestine has been examined. It was found that the guinea pig is without a transport system for non-alpha-amino acids. The transport of imino acids was characterized using methylaminoisobutyrate (MeAIB) as a substrate. This choice was validated by lack of kinetic evidence that more than one transport system was involved in the transport of MeAIB, by the identical values of the estimates of the passive permeability of MeAIB, the magnitude of its proline-resistant transport, and the permeability of mannitol. The transport system for MeAIB is moderately stereospecific. It does not accept cationic amino acids. It accepts alpha-amino-monocarboxylic acids but N-methylation increases the affinities of these amino acids by an order of magnitude. The length of the side-chain of the aliphatic imino acids seems of little importance for the affinity for the transport system, but the data on inhibition of the transport of MeAIB by proline and piperidine-2-carboxylic acid indicate that it is sharply increased by ring formation.
D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active; D006168:Guinea Pigs; D007098:Imino Acids / Q000378:metabolism*; D007421:Intestine, Small / Q000648:ultrastructure*; D007930:Leucine / Q000378:metabolism; D008433:Mathematics; D008745:Methylation; D008871:Microvilli / Q000378:metabolism; D013329:Structure-Activity Relationship; D015091:beta-Alanine / Q000378:metabolism
D000621:Aminoisobutyric Acids; D007098:Imino Acids; D015091:beta-Alanine; C017911:2-(methylamino)isobutyric acid; D007930:Leucine