Adhesion of human platelets to collagen: evidence for two states of platelet activation.
Normal human platelets suspended in buffer were exposed to a suspension of bovine tendon collagen fibrils and the rate of adhesion observed over a period of 60 minutes. In fresh platelet preparations, approximately 15-20% of the platelets adhered within one minute, and the total percent adhesion, following pseudo first order kinetics after the first minute, approached 60% after 60 minutes. Pre-treatment with N-ethylmaleimide, dithiothreitol, and glutaraldehyde reduced adhesion at 1 minute to less than 5%, but only N-ethylmaleimide completely blocked adhesion at 60 minutes. Thus, freshly collected, washed human platelets contain two distinct subpopulations exhibiting greatly differing rates of adhesion, reflecting different levels of reactivity toward collagen.
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002417:Cattle; D003094:Collagen / Q000378:metabolism*; D003847:Deoxyglucose / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D010973:Platelet Adhesiveness / Q000187:drug effects
D003094:Collagen; D003847:Deoxyglucose; D005976:Glutaral