Amino acid sequence diversity in mouse lambda 2 variable regions.
The lambda-chains of immunoglobulins from BALB/c mice constitute the simplest system presently available for studying patterns of variable-region diversity. The limited number of V lambda and J lambda germ-line gene segments facilitates comparison of expressed and germ-line sequences. We report here the complete amino acid sequence of the variable regions of three lambda 2 chains and of one chain representing a V lambda 2----J lambda 3 rearrangement. Together with the previously determined sequence of the lambda 2 chain from myeloma MOPC-315, the results illustrate the following types of variable-region diversification: expression of a single V gene segment with more than one J segment, variability at the V-J junction, and presumably, somatic mutation in V and in J. The extent of somatic diversification in these lambda 2 chains is limited, consistent with results obtained previously with lambda 1 chains.
D000595:Amino Acid Sequence; D000818:Animals; D000916:Antibody Diversity; D005815:Genetic Code; D007144:Immunoglobulin J-Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000032:analysis / Q000235:genetics*; D007146:Immunoglobulin lambda-Chains / Q000032:analysis / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000302:isolation & purification; D014357:Trypsin / Q000494:pharmacology
D007144:Immunoglobulin J-Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains; D010446:Peptide Fragments; D014357:Trypsin