title: Adherence of murine peripheral blood eosinophils and neutrophils to the different parasitic stages of Nematospiroides dubius.
abstract: Eosinophils and neutrophils, purified by density gradient centrifugation from the blood of infected mice resistant to reinfection, were tested for their ability to adhere to the different parasitic larval stages of the murine nematode parasite Nematospiroides dubius. Cells were tested for adherence to larvae which had been sensitised with immune mouse serum (IMS) or normal mouse serum (NMS) in the presence of CA2+ and Mg2+ ions. EDTA, or EGTA. Differences were observed in the degree of cell adherence to the different stages of the parasite. However, the adherence of the two cell types to any given stage of the parasite was similar. Adherence to the sheathed infective third-stage (L3) larvae, 96 h post-infective larvae and to adult worms depended to a large degree on conditions suitable for complement activation (viz. fresh serum and the presence of Ca2+ and Mg2+ ions). Complement was activated both via the alternative pathway by the parasite itself and via the classical pathway by parasite-bound antibodies. In these conditions, cell adherence probably occurred predominantly through the interaction of leucocyte third component of complement (C3) receptors with parasite-bound C3. In contrast, adherence of cells to exsheathed L3 and to the 48 h and 72 h post-infective larval stages appeared to involve antibody/Fc receptor as well as C3/C3 receptor interaction. The data indicate that N. dubius may undergo a series of antigenic changes during its life cycle and that antibodies capable of mediating granulocyte attachment are elicited predominantly against the early tissue developmental forms of the parasite.
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003170:Complement Pathway, Alternative / Q000187:drug effects; D003171:Complement Pathway, Classical / Q000187:drug effects; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004804:Eosinophils / Q000276:immunology*; D006801:Humans; D007814:Larva; D016177:Macrophage-1 Antigen; D008274:Magnesium / Q000494:pharmacology; D051379:Mice; D009348:Nematoda / Q000254:growth & development / Q000276:immunology*; D009349:Nematode Infections / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D051381:Rats; D011951:Receptors, Complement / Q000276:immunology; D011961:Receptors, Fc / Q000276:immunology
chemical_list: D016177:Macrophage-1 Antigen; D011951:Receptors, Complement; D011961:Receptors, Fc; D004533:Egtazic Acid; D004492:Edetic Acid; D008274:Magnesium; D002118:Calcium