Involvement of prolines-114 and -117 in the slow refolding phase of ribonuclease A as determined by isomer-specific proteolysis.
Using the method of isomer-specific proteolysis (ISP), the cis-trans nature of the peptide bonds involving prolines-114 and -117 in ribonuclease (RNase) has been investigated. These studies involve the pretreatment of RNase first with either a short pepsin pulse or a short mercaptoethanol pulse to irreversibly unfold the protein and then with a short chymotrypsin pulse to quickly cleave the Tyr115-Val116 bond so that the chain is suitably trimmed for the subsequent stereospecific cleavage either by aminopeptidase P, to investigate proline-117, or by a proline-specific endopeptidase, to investigate proline-114. The most reasonable interpretation of our results suggests that proline-117 is essentially 100% trans in both the native and unfolded states, so it apparently makes no direct contribution to the slow refolding kinetics of RNase. It is also determined that proline-114 is 100% cis in native RNase and ca. 95% cis in reversibly unfolded RNase so only 5% of the unfolded RNase can be rate limited by trans to cis isomerization of proline-114 during refolding. Careful spectroscopic studies of refolding show that the smallest and slowest of the refolding phases, the ct phase, has the proper amplitude (5%), relaxation time (400 s at 10 degrees C), and activation energy (17 kcal) for a phase that is rate limited by the trans to cis isomerization of proline-114. Measurements of the kinetics of binding of cytidine 2'-monophosphate during refolding further show that RNase does not become active until proline-114 has isomerized to the native cis configuration. It is concluded that none of the three prolines thus far examined (i.e., prolines-93, -114, and -117) by the ISP method is involved in the formation of a fully active, nativelike intermediate which has "incorrect" proline isomers. The specific structural process which is responsible for the largest of the three slow refolding phases, the XY phase, is still undetermined. Although ISP results on proline-42 are not yet available, it seems possible that this slow phase may be rate limited by a process other than proline isomerization. In unrelated studies, results from chymotrypsin hydrolyses of several short peptides containing the sequence -X-Y-Pro- show that cleavage of an active X-Y bond is very slow when it is immediately adjacent on the amino side of a proline peptide bond. Thus, chymotrypsin cleavage may not be generally useful as the analytical step in isomer-specific proteolysis.
D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000378:metabolism; D002918:Chymotrypsin / Q000378:metabolism; D003568:Cytidine Monophosphate / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D006868:Hydrolysis; D007536:Isomerism; D007700:Kinetics; D008623:Mercaptoethanol / Q000494:pharmacology; D010434:Pepsin A / Q000494:pharmacology; D010447:Peptide Hydrolases / Q000378:metabolism*; D011392:Proline; D000085822:Prolyl Oligopeptidases; D011487:Protein Conformation / Q000187:drug effects; D012259:Ribonuclease, Pancreatic / Q000378:metabolism*; D012697:Serine Endopeptidases; D013053:Spectrophotometry
D008623:Mercaptoethanol; D011392:Proline; D012259:Ribonuclease, Pancreatic; D010450:Endopeptidases; D010447:Peptide Hydrolases; D000626:Aminopeptidases; C022750:X-Pro aminopeptidase; D012697:Serine Endopeptidases; D002918:Chymotrypsin; D000085822:Prolyl Oligopeptidases; D010434:Pepsin A; D003568:Cytidine Monophosphate