Complete covalent structure of human platelet factor 4.
The amino acid sequence of the subunit of human platelet factor 4 has been determined. Human platelet factor 4 consists of identical subunits containing 70 amino acids, each with a molecular weight of 7,756. The molecule contains no methionine, phenylalanine or tryptophan. The proposed amino acid sequence of PF4 is: Glu-Ala-Glu-Glu-Asp-Gly-Asp-Leu-Gln-Cys-Leu-Cys-Val-Lys-Thr-Thr-Ser-Gln-Val-Arg-Pro-Arg-His-Ile-Thr-Ser-Leu-Glu-Val-Ile-Lys-Ala-Gly-Pro-His-Cys-Pro-Thr-Ala-Gln-Leu-Ile-Ala-Thr-Leu-Lys-Asn-Gly-Arg-Lys-Ile-Cys-Leu-Asp-Leu-Gln-Ala-Pro-Leu-Tyr-Lys-Lys-Ile-Ile-Lys-Lys-Leu-Leu-Glu-Ser. From consideration of the homology with beta-thromboglobulin, disulphide bonds between residues 10 and 36 and between residues 12 and 52 can be inferred.
D000595:Amino Acid Sequence; D001779:Blood Coagulation Factors; D001792:Blood Platelets / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D006801:Humans; D006898:Hydroxylamines / Q000494:pharmacology; D008970:Molecular Weight; D010455:Peptides; D010978:Platelet Factor 4 / Q000302:isolation & purification; D011509:Proteoglycans; D014357:Trypsin / Q000494:pharmacology; D001620:beta-Thromboglobulin
D001779:Blood Coagulation Factors; D006898:Hydroxylamines; D010455:Peptides; D011509:Proteoglycans; D001620:beta-Thromboglobulin; D010978:Platelet Factor 4; D014357:Trypsin