Studies directed toward testing the "channeling" hypothesis--ribonucleotides----DNA in leukemia L1210 cells.
Experiments were carried out to test for the presence of "channeling" in L1210 cells. L1210 cells were incubated in culture in the presence of labeled cytidine and "cold" deoxycytidine and conversely, in the presence of labeled deoxycytidine and "cold" cytidine. Cytidine did not inhibit the incorporation of [14C]deoxycytidine into DNA while deoxycytidine decreased the incorporation of [14C]cytidine into DNA. Further, in L1210 cells there was not a coordinate inhibition of thymidylate synthetase when either DNA polymerase was inhibited (aphidicolin) or ribonucleotide reductase was inhibited (hydroxyurea). These data indicate that leukemia L1210 cells do not selectively channel ribonucleotides to DNA through a tightly coupled enzyme complex.
D000818:Animals; D016590:Aphidicolin; D003562:Cytidine / Q000378:metabolism; D004261:DNA Replication; D003841:Deoxycytidine / Q000378:metabolism; D004224:Diterpenes / Q000494:pharmacology; D006918:Hydroxyurea / Q000494:pharmacology; D007939:Leukemia L1210 / Q000201:enzymology*; D051379:Mice; D009097:Multienzyme Complexes / Q000378:metabolism*; D019384:Nucleic Acid Synthesis Inhibitors; D012264:Ribonucleotide Reductases / Q000037:antagonists & inhibitors; D012265:Ribonucleotides / Q000378:metabolism*; D013940:Thymidylate Synthase / Q000037:antagonists & inhibitors
D004224:Diterpenes; D009097:Multienzyme Complexes; D019384:Nucleic Acid Synthesis Inhibitors; D012265:Ribonucleotides; C037117:replitase; D003841:Deoxycytidine; D016590:Aphidicolin; D003562:Cytidine; D012264:Ribonucleotide Reductases; D013940:Thymidylate Synthase; D006918:Hydroxyurea