title: The synthesis of cyclooxygenase products in ocular tissues of various species.
abstract: In the present study we compared the synthesis of various cyclooxygenase products in albino rabbit, dog (puppy and adult), cat, cow and human ocular tissues from 14C-arachidonic acid (AA). Albino rabbit conjunctiva synthesized a substantial amount (47%) of cyclooxygenase products, while cat and dog (puppy as well as adult) conjunctival tissues synthesized lesser but significant amounts of cyclooxygenase products from 14C-AA. Cyclooxygenase activity in the anterior uvea of different species tested was in the following order of potency: cat greater than albino rabbit greater than dog greater than human greater than cow. Furthermore, human and cow anterior uvea synthesized significantly lesser amount of cyclooxygenase products from 14C-AA than amounts from an intermediate substrate, 14C-PGH2. Interestingly, cow anterior uvea synthesized PGF2 alpha and 6-keto-PGF1 alpha (a stable metabolite of PGI2) while human anterior uvea synthesized all cyclooxygenase products from 14-C-PGH2.
mesh_terms: D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002415:Cats; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003228:Conjunctiva / Q000201:enzymology*; D004285:Dogs; D006801:Humans; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D011453:Prostaglandins / Q000096:biosynthesis; D013045:Species Specificity; D014602:Uvea / Q000201:enzymology*
chemical_list: D001095:Arachidonic Acids; D011453:Prostaglandins; D016718:Arachidonic Acid; D011451:Prostaglandin-Endoperoxide Synthases