Enhanced production of human gamma-interferon in nylon column-fractionated cell cultures.
The production of human gamma-interferon (HuIFN-gamma) in unfractionated and nylon wool column-fractionated leukocyte cell cultures stimulated with PMA and PHA was investigated. Production was studied with normal and reduced autologous serum protein levels in 96-hr spinner cultures. A 10- to 15-fold enhancement of production and a 50-fold increase in specific activity of crude HuIFN-gamma was demonstrated in nylon column-fractionated/reduced serum cell cultures. Kinetic analysis revealed a production rate maximum within 6 hr of induction in unprocessed cell cultures, whereas production occurred at an essentially constant rate for 48 hr in fractionated cell cultures.
D001798:Blood Proteins / Q000378:metabolism; D002374:Catalase / Q000494:pharmacology; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007700:Kinetics; D007962:Leukocytes / Q000378:metabolism*; D008214:Lymphocytes; D009504:Neutrophils; D010835:Phytohemagglutinins / Q000494:pharmacology
D001798:Blood Proteins; D010835:Phytohemagglutinins; D007371:Interferon-gamma; D002374:Catalase