Factor IXa protects activated factor VIII against inactivation by activated protein C.
The effect of activated protein C on human coagulation factor VIII was evaluated by studying its effect on the intrinsic factor X activation using a system of purified coagulation factors (factor IXa, factor X, factor VIII, activated protein C). Activated protein C had no effect on the activation of factor X by factor IXa in the absence of factor VIII. In the presence of thrombin activated factor VIII the rate of factor X activation was decreased by activated protein C in a dose dependent way. The presence of factor IXa during the preincubation of factor VIII with activated protein C was found to protect the factor VIII against inactivation. The results suggest that activated protein C and factor IXa compete for the same part of the factor VIII molecule.
D002118:Calcium / Q000378:metabolism; D005164:Factor IX / Q000494:pharmacology*; D015949:Factor IXa; D005169:Factor VIII / Q000378:metabolism*; D005170:Factor X / Q000378:metabolism; D006023:Glycoproteins / Q000494:pharmacology*; D006801:Humans; D010743:Phospholipids / Q000378:metabolism; D011486:Protein C
D006023:Glycoproteins; D010743:Phospholipids; D011486:Protein C; D005169:Factor VIII; D005164:Factor IX; D005170:Factor X; D015949:Factor IXa; D002118:Calcium