Determination of tolrestat, a novel aldose reductase inhibitor, in serum and tissues.
An ultraviolet spectrophotometric method and a high-performance liquid chromatographic (HPLC) method were developed for the determination of tolrestat, a novel aldose reductase inhibitor, in serum. The limits of detection of the methods are 15 micrograms/ml and 0.2 microgram/ml, respectively. With human serum, a modification of the HPLC method provides sensitivity to 25 ng/ml. The specificity of the methods were compared. The HPLC method can be applied to the lens and the sciatic nerve.
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D004355:Drug Stability; D006801:Humans; D007908:Lens, Crystalline / Q000032:analysis; D009281:Naphthalenes / Q000032:analysis* / Q000097:blood; D051381:Rats; D012584:Sciatic Nerve / Q000032:analysis; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013401:Sugar Alcohol Dehydrogenases / Q000037:antagonists & inhibitors*
D009281:Naphthalenes; C040550:tolrestat; D013401:Sugar Alcohol Dehydrogenases; D000449:Aldehyde Reductase