The effect of polyunsaturated fatty acids on endothelial cells and their production of prostacyclin, thromboxane and platelet inhibitory activity.
Primary cultures of human endothelial cell monolayers were incubated with albumin-bound fatty acids of the omega-3 and omega-6 families for a maximum of 24 hrs, to investigate the production of 6-keto-PGF1 alpha, TXB2 and platelet inhibitory activity (PIA). Arachidonic acid was a potent stimulator of all parameters. The release of 6-keto-PGF1 alpha was significantly reduced by equimolar concentrations of linoleic, dihomogamma linolenic and eicosapentaenoic acids, but not by linolenic acid. PIA was not similarily affected. Dihomogamma linolenic acid was also a weak stimulator of 6-keto-PGF1 alpha and PIA, but reduced the content of both in the cells after 24 hrs. Eicosapentaenoic and docosahexaenoic acids both depressed 6-keto-PGF1 alpha production but PIA was maintained after 24 hrs. Indomethacin always blocked 6-keto-PGF1 alpha and PIA production. None of the effects correlated to release of 51CR from prelabelled cells.
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D002478:Cells, Cultured; D004727:Endothelium / Q000166:cytology / Q000378:metabolism; D011464:Epoprostenol / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D013929:Thromboxane B2 / Q000378:metabolism*; D013931:Thromboxanes / Q000378:metabolism*
D001095:Arachidonic Acids; D005231:Fatty Acids, Unsaturated; D013931:Thromboxanes; D016718:Arachidonic Acid; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol; D007213:Indomethacin