One-step purification of hybrid proteins which have beta-galactosidase activity.
A one-step purification method of hybrid proteins exhibiting beta-galactosidase activity, based on affinity chromatography in the presence of high salt concentration, is described. Starting from crude bacterial extracts, several milligrams of near-homogeneous proteins can be obtained in a few hours with an overall yield of 85 to 95%. The purified hybrid proteins can be used to obtain antibodies against the foreign portion of the protein fusion.
D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005696:Galactosidases / Q000235:genetics*; D005796:Genes; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D001616:beta-Galactosidase / Q000235:genetics* / Q000302:isolation & purification
D004274:DNA, Recombinant; D005696:Galactosidases; D001616:beta-Galactosidase