Novel kappa light-chain gene rearrangements in mouse lambda light chain-producing B lymphocytes.
The genes that encode the immunoglobulin proteins made by B lymphocytes are made up of segments that are separately encoded in the germ-line genome and brought together by recombination during B-cell ontogeny. There are two types of immunoglobulin light chain, kappa and lambda, but only a single type is expressed in individual B cells. It is thought that kappa gene recombination precedes lambda gene recombination during B-cell ontogeny. We describe here unusual recombinations that have occurred in two lambda-producing B-cell lines and suggest that they are involved in the developmental switch from kappa to lambda gene expression in maturing B cells. These recombinations involve the J kappa-C kappa introns of V-J joined but nonfunctional kappa genes and a sequence that in the germ line occurs downstream of the C kappa exon (called RS, for recombining sequence).
D001402:B-Lymphocytes / Q000502:physiology*; D002454:Cell Differentiation; D005786:Gene Expression Regulation; D005796:Genes; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D007146:Immunoglobulin lambda-Chains / Q000235:genetics*; D011995:Recombination, Genetic
D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains