[Isolation, purification and physicochemical properties of pesticin I from cells of Yersinia pestis strain EV].
Pesticin I has been isolated and purified from Y. pestis strain EV. The homogeneous preparation of Pesticin has been shown to be monomer protein with a molecular weight of 65000 daltons, having three immunologically identical alpha-, beta- and gamma-forms with different isoelectric points. The amino acid composition of Pesticin I is presented. Rabbit anti-serum to the beta-form of the preparation of Pesticin has been obtained.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001430:Bacteriocins / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000187:drug effects; D007114:Immunization; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D011817:Rabbits; D015010:Yersinia pestis
D000596:Amino Acids; D001430:Bacteriocins; C011330:Pesticin
Vydelenie, ochistka i nekotorye fiziko-khimicheskie svoĭstva pestitsina I iz kletok shtamma EV Yersinia pestis.