[Activation of oxidative metabolism of granulocytes and monocytes by IGG-coated platelets from patients with thrombocytopenia].
We have previously shown that monoclonal anti-T cell antibodies bound to their specific targets can trigger the activation of monocyte/macrophage oxidative metabolism through an Fc receptor-mediated interaction. The present study demonstrates that IgG coated platelets from patients with thrombocytopenia-associated diseases can induce a similar respiratory burst activation in polymorphonuclear and mononuclear phagocytes from normal individuals. The intensity of the oxidative reaction as measured by luminol-dependent chemiluminescence is in close correlation with the level of surface-bound IgG molecules as determined by a radioactive anti-immunoglobulin assay. This new methodology to evaluating IgG fixed on human platelets by their capacity to trigger the generation of highly reactive oxygen species by granulocytes and monocytes has also suggested a new mechanism in the genesis of thrombocytopenia associated with autoimmune diseases.
D002462:Cell Membrane / Q000276:immunology; D006098:Granulocytes / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007700:Kinetics; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D010101:Oxygen Consumption; D011961:Receptors, Fc / Q000502:physiology*; D012016:Reference Values; D013922:Thrombocytosis / Q000276:immunology*
D007074:Immunoglobulin G; D011961:Receptors, Fc
Activation du métabolisme oxydatif des granulocytes et des monocytes par des plaquettes recouvertes d'IgG provenant de patients porteurs de thrombopénies.