Standardization of parameters for the mycobacillin synthetase activity.
An effective method of preparation involving sonication was developed for cell-free mycobacillin synthetase from Bacillus subtilis. The enzyme showed optimum activity at a buffer concentration of 50 mM (Tris-HCl) and pH 7.5. ATP and Mg2+ which were essential for synthesis showed an optimum requirement at a ratio of 1:1. The synthetase was markedly inhibited by ADP whereas AMP was without any effect. ATP or ATP-generating system could not be replaced by GTP, UTP or CTP. Co2+ and Mn2+ could to some extent substitute Mg2+. Mercapto reagents inhibited the antibiotic synthesis. Exogenous addition of pantothenic acid had no effect.
D001412:Bacillus subtilis / Q000201:enzymology*; D002250:Carbon Radioisotopes; D007700:Kinetics; D009097:Multienzyme Complexes; D009158:Mycobacillin / Q000096:biosynthesis; D010453:Peptide Synthases / Q000302:isolation & purification / Q000378:metabolism*; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D002250:Carbon Radioisotopes; D009097:Multienzyme Complexes; D013439:Sulfhydryl Reagents; D009158:Mycobacillin; D010453:Peptide Synthases; C042173:mycobacillin synthetase