Effects of divalent cations and functionally related substances on the yeast to mycelium transition in Sporothrix schenckii.
In a minimal basal medium with glucose at pH 4.0 and 25 degrees C, a lowering of the magnesium and zinc concentrations or increase in the calcium concentration of the medium favoured the yeast-mycelium transition in Sporothrix schenckii. Addition of zinc (1 and 10 mM) inhibited mycelial development and induced reversion to a yeast-like morphology. EDTA and EGTA also delayed germ tube formation, possibly by their calcium-chelating effects or by altering intracellular concentrations of this or other ions. Ionophore X537A also caused a delay in germ tube formation, possibly by interfering with magnesium metabolism in these cells.
D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent / Q000494:pharmacology*; D002614:Chelating Agents / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D007832:Lasalocid / Q000494:pharmacology*; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D013175:Sporothrix / Q000166:cytology* / Q000187:drug effects; D015032:Zinc / Q000494:pharmacology
D002413:Cations, Divalent; D002614:Chelating Agents; D008345:Manganese; D004533:Egtazic Acid; D004492:Edetic Acid; D008274:Magnesium; D015032:Zinc; D002118:Calcium; D007832:Lasalocid