Kinetic studies of the activation of factor X by factors IXa and VIII:C in the absence of thrombin.
The activation of factor X by the components of the intrinsic coagulation pathway has been studied using a preparation of bovine factor VIII which did not require 'activation' with thrombin prior to its participation in the reaction. Measurements of the kinetic parameters indicated that factor VIII:C stimulated the formation of factor Xa via a 170-fold increase in the Vmax; factor VIII:C had little effect on the Km for factor X.
D000818:Animals; D000941:Antigens / Q000502:physiology*; D001777:Blood Coagulation; D002417:Cattle; D005164:Factor IX / Q000502:physiology*; D015949:Factor IXa; D005169:Factor VIII / Q000276:immunology* / Q000502:physiology; D005170:Factor X / Q000378:metabolism*; D007700:Kinetics; D013917:Thrombin
D000941:Antigens; D005169:Factor VIII; D005164:Factor IX; D005170:Factor X; D015949:Factor IXa; D013917:Thrombin