title: Technical note: improved preparation of fine needle aspiration biopsies for transmission electron microscopy.
abstract: An improved method of cell concentration for ultramicrotomy is described. It is based on centrifugation of glutaraldehyde-fixed fine needle aspirates in 10% bovine albumin. Sampling of comparatively small numbers of tumour cells or tissue fragments is enhanced, particularly in the presence of heavy blood contamination. This results in a marked improvement in the proportion of diagnostically useful electron microscopic assessments.
mesh_terms: D001707:Biopsy, Needle / Q000379:methods*; D002499:Centrifugation, Density Gradient; D003581:Cytodiagnosis / Q000379:methods; D005976:Glutaral; D006801:Humans; D008854:Microscopy, Electron / Q000379:methods; D008867:Microtomy; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000648:ultrastructure; D012710:Serum Albumin, Bovine
chemical_list: D012710:Serum Albumin, Bovine; D005976:Glutaral