Methylation of newly synthesized ribosomal protein L11 in a DNA-directed in vitro system.
The methylation of newly synthesized ribosomal protein L11 has been obtained in an in vitro system using lambda rifd 18 DNA as template and S-adenosyl[3H]methionine as methyl donor. About four methyl groups are incorporated per mol of L11 synthesized and the bulk of the methyl groups are present in the protein as trimethyl-lysine. lambda rifd 18 DNA also contains the genes for rRNA and newly synthesized 16 S and 23 S RNA are also methylated in this in vitro system.
D010582:Bacteriophage lambda / Q000378:metabolism*; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D008745:Methylation; D008970:Molecular Weight; D012269:Ribosomal Proteins / Q000096:biosynthesis*; D012436:S-Adenosylmethionine / Q000378:metabolism; D014161:Transduction, Genetic
D004269:DNA, Bacterial; D004279:DNA, Viral; D012269:Ribosomal Proteins; D012436:S-Adenosylmethionine