Anti-mRNA: specific inhibition of translation of single mRNA molecules.
A plasmid was constructed to generate RNA complementary to the beta-galactosidase mRNA under control of the phage lambda PL promoter. When this anti-mRNA was produced, synthesis of beta-galactosidase was dramatically inhibited (98%). Syntheses of galactoside permease and transacetylase, whose coding sequences are downstream of the beta-galactosidase coding region, are inhibited to a lesser degree, 80% and 55%, respectively. The generation of anti-mRNA that can be targeted to inhibit a single species of mRNA molecule within cells provides a potent mechanism by which specific transcripts can be translationally inactivated. This can be used to determine the function of proteins as well as to select cloned genes in a single rapid and convenient step.
D000123:Acetyltransferases / Q000235:genetics; D000469:Alkaline Phosphatase / Q000235:genetics; D000936:Antigen-Antibody Complex; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D007106:Immune Sera; D007763:Lac Operon; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000276:immunology; D001616:beta-Galactosidase / Q000235:genetics
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D012333:RNA, Messenger; D000123:Acetyltransferases; C022770:galactoside acetyltransferase; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase