Stimulation of RNA polymerases from mouse spleen by polyamines and its modification by ammonium sulfate.
Nuclear RNA polymerases Ia, Ib, II and III purified from spleen of Swiss albino mice (Mus musculus) were stimulated significantly by the polyamines, spermine and spermidine, in the presence of ammonium sulfate in vitro. In the absence of ammonium sulfate, the optimal stimulating concentrations of both the polyamines for the RNA polymerases were generally diminished and more physiological. 8 mM spermine stimulated both RNA polymerases Ia and II in the presence of sulfate ions, but inhibited both enzymes significantly in the absence of sulfate ions. Stimulation of mouse spleen RNA polymerase II by spermine affects elongation of RNA chains whereas inhibition by spermine affects initiation of RNA synthesis.
D000645:Ammonium Sulfate / Q000494:pharmacology*; D000818:Animals; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D011073:Polyamines / Q000494:pharmacology*; D011700:Putrescine / Q000494:pharmacology; D012318:RNA Polymerase I / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism; D012320:RNA Polymerase III / Q000378:metabolism; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology; D013154:Spleen / Q000201:enzymology*
D011073:Polyamines; D013096:Spermine; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I; D012320:RNA Polymerase III; D000645:Ammonium Sulfate; D013095:Spermidine; D011700:Putrescine