Surface antigens specifically expressed by activated T cells in humans.
By immunizing BALB/c mice with human T cells derived from a secondary mixed lymphocyte reaction (MLR) and successive fusion of the spleen cells with P3X63Ag8U1 myeloma cells, several monoclonal antibodies to activated T cells were obtained. Four of them (MLR 1-4) were shown to be specific for activated T cells only, showing no reactivity with peripheral blood cells. The antigens recognized by these antibodies are differently distributed and appear on T cells at different times after stimulation. Thus, MLR2 stains nearly all the responder T cells present in a secondary MLR, while MLR 1, 3 and 4 stain only a fraction. MLR2 and 3 are also present on mitogen-stimulated cells. At least 3 of these MLR antigens may define different sets of activated T cells, and are likely to become useful in monitoring T cell activation also in the course of diseases involving the immune system.
D000818:Animals; D000906:Antibodies; D000954:Antigens, Surface / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D006680:HLA Antigens; D006801:Humans; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*
D000906:Antibodies; D000954:Antigens, Surface; D006680:HLA Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M