Some characteristics of apurinic sites in single- and double-stranded biologically active phi X174 DNA.
With biologically active DNA of the bacteriophage phi X174, both single and double-stranded, some physico-chemical and biological parameters of the depurination reaction are studied. It is shown that in single-stranded DNA each apurinic site is lethal, while in double-stranded RFI-DNA only about 5% of these sites are lethal. Furthermore it is concluded that the apurinic sites are formed at different rates in single- and double-stranded DNA and also the conversion into breaks of the apurinic sites is different for both forms of DNA.
D001080:Apurinic Acid; D010584:Bacteriophage phi X 174 / Q000032:analysis*; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D006863:Hydrogen-Ion Concentration; D007700:Kinetics
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D001080:Apurinic Acid