Microinjection studies of protein transit across the nuclear envelope of human cells.
Proteins of various molecular weights were conjugated with rhodamine and microinjected into the cytoplasm or nucleus of HeLa cells. The injected proteins were then localized within the cells at various times thereafter with fluorescence microscopy. Proteins below approx. 60 kD rapidly crossed the HeLa nuclear envelope. Some larger proteins also were able to pass into or out of the nucleus, while others were unable to do so, indicating the selective permeability of the HeLa nuclear envelope to large proteins. The nuclear protein HMG17 accumulated within the nucleus shortly after cytoplasmic microinjection.
D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism*; D004396:Coloring Agents; D003593:Cytoplasm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008845:Microinjections; D009685:Nuclear Envelope / Q000378:metabolism*; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D012235:Rhodamines
D004396:Coloring Agents; D011506:Proteins; D012235:Rhodamines; C009434:tetramethylrhodamine isothiocyanate