Determination of polyamine oxidase activities in human tissues.
A very simple fluorometric assay for polyamine oxidase (PAO) in tissues, with N1-monoacetylspermine as substrate, is described. The PAO was present in all human organs tested; it was highest in the liver, followed by the testis, kidney, spleen and small intestine.
D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007421:Intestine, Small / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013154:Spleen / Q000201:enzymology; D013379:Substrate Specificity; D013737:Testis / Q000201:enzymology; D014018:Tissue Distribution; D000097667:Polyamine Oxidase
D000587:Oxidoreductases Acting on CH-NH Group Donors; D000097667:Polyamine Oxidase