Chromatographic behaviour of the molecular forms of guinea-pig skeletal muscle cytoplasmic malate dehydrogenase.
The isolated molecular forms of guinea-pig skeletal muscle cytoplasmic malate dehydrogenase have a different chromatographic behaviour through affinity or hydrophobic interaction gels; in all cases the retention of the B form is more noticeable. Chromatography of a partly purified preparation through 5' AMP-Sepharose allows both molecular forms of malate dehydrogenase to be separated and obtained free from lactate dehydrogenase.
D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003593:Cytoplasm / Q000201:enzymology; D004588:Electrophoresis, Cellulose Acetate; D006168:Guinea Pigs; D007527:Isoenzymes / Q000302:isolation & purification*; D008291:Malate Dehydrogenase / Q000302:isolation & purification*; D009132:Muscles / Q000201:enzymology*
D007527:Isoenzymes; D008291:Malate Dehydrogenase