Electrophoretic patterns of proteins in cystic fibrosis sweat.
The abnormal gene product in cystic fibrosis has not been identified. We report that two-dimensional polyacrylamide gel electrophoresis of 125I-labeled sweat proteins coupled with fluorography and rare earth screen radioautography reveals an absence of an acidic protein of molecular weight 60,000 in sweat collected from 9 patients with cystic fibrosis. The protein spot was present in sweat from heterozygotes and from controls.
D002648:Child; D003550:Cystic Fibrosis / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006579:Heterozygote; D006801:Humans; D008297:Male; D011506:Proteins / Q000032:analysis*; D013542:Sweat / Q000032:analysis*
D011506:Proteins