Complex formation of platelet membrane glycoproteins IIb and IIIa with fibrinogen.
We have recently reported the isolation of purified platelet membrane glycoproteins IIb and IIIa and the generation of monospecific antisera to these membrane proteins. Using these monospecific antisera in an enzyme-linked immunosorbent assay system, it is no demonstrated that glycoprotein IIb (GPIIb) and glycoprotein IIIa (GPIIIa) form a complex with purified human fibrinogen. The formation of this GPIIb-GPIIIa fibrinogen complex is calcium dependent, fibrinogen specific, saturable, and inhibited by specific amino sugars and amino acids. These observations suggest that the GPIIb-GPIIIa macromolecular complex on the platelet surface acts under the proper physiologic circumstances as the fibrinogen binding site required for normal platelet aggregation.
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D001792:Blood Platelets / Q000032:analysis*; D002118:Calcium / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005340:Fibrinogen / Q000378:metabolism*; D005944:Glucosamine / Q000494:pharmacology; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D006595:Hexosamines / Q000494:pharmacology; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism; D008358:Mannose / Q000031:analogs & derivatives / Q000494:pharmacology; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D010980:Platelet Membrane Glycoproteins; D011817:Rabbits; D019699:Thrombospondins
D000596:Amino Acids; D006023:Glycoproteins; D006595:Hexosamines; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D008565:Membrane Proteins; D010980:Platelet Membrane Glycoproteins; D019699:Thrombospondins; C008322:mannosamine; D005340:Fibrinogen; D005944:Glucosamine; D008358:Mannose; D002118:Calcium