[Characteristics of the gene transcription of the intracisternal A-particles].
A clone containing the gene of intracisternal A-particles (IAP-gene) was isolated by hybridization with type A double-stranded RNA from the bank of fragments of genome mouse DNA cloned in pBR322 plasmid. Using DNA fragments of this clone we studied transcription of IAP-genes in cells of MOPC-21 plasmocytoma and Ehrlich ascitic carcinoma. Transcription was shown to be partially symmetrical, with predominant transcription of one of DNA chains. The observed transcripts could not be associated with known variants of IAP-genes. The sizes of RNAs transcribed in MOPC-21 and Ehrlich ascitic carcinoma cells and their sequences differed significantly. Apparently, minor variants of IAP-genes are transcribed in the cells under study.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000382:microbiology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D007181:Inclusion Bodies, Viral; D051379:Mice; D009693:Nucleic Acid Hybridization; D010954:Plasmacytoma / Q000382:microbiology; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D014158:Transcription, Genetic; D014771:Virion / Q000235:genetics*
D004273:DNA, Neoplasm; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D004247:DNA
Osobennosti transkriptsii genov intratsisternal'nykh A-chastits.