Vasopressin rapidly stimulates Ca2+ efflux from intracellular pool in quiescent Swiss 3T3 cells.
Addition of the neurohypophysial nonapeptide [Arg8]vasopressin to 45Ca2+-loaded quiescent cultures of Swiss 3T3 cells or diploid mouse embryo fibroblasts causes a rapid increase (within seconds) in the rate of 45Ca2+ efflux from the cells. This reflects the release of 45Ca2+ from an intracellular store that is partly or entirely derived from mitochondria and leads to a marked decrease (52%) in the intracellular 45Ca2+ content of the cultures under equilibrium 45Ca2+ conditions. The effect is dose dependent, specific, and blocked by a vasopressor antagonist. Prolonged pretreatment with vasopressin that renders the cells unresponsive to the mitogenic effects of the hormone also blocks the Ca2+ response. The results suggest that Ca2+ fluxes may be involved in the mechanism of mitogenic action of vasopressin in Swiss 3T3 cells.
D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D008934:Mitogens / Q000494:pharmacology; D051381:Rats; D013268:Stimulation, Chemical; D013696:Temperature; D013997:Time Factors
D008934:Mitogens; D001127:Arginine Vasopressin; D002118:Calcium