Perturbation of early development of the immune system by normal adult lymphoid cells.
Seven-day-old C57BL/10 mice injected with 3 X 10(6) normal adult lymph node cells (NAL) from syngeneic donors were compared with uninjected littermates when 5 to 7 wk old. The direct PFC response to sheep erythrocytes was suppressed by 60%. Responses to PHA and Con A but not LPS were suppressed by 60 to 90%. Primary and secondary (after skin graft rejection) MLR responses were suppressed by 30 to 40%. Active suppressor cells were demonstrable in these mice. Skin grafts incompatible for H-2 were rejected in normal time. NAL derived from adult mice conferred the effect, but not cells from 7-day-old donors. Mice inoculated with Rous sarcoma virus within the 1st day of life and 3 X 10(6) NAL at 7 days of age displayed up to 5-fold increased incidence of primary sarcomas.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D006084:Graft Rejection; D006462:Hemolytic Plaque Technique; D008070:Lipopolysaccharides / Q000494:pharmacology; D008198:Lymph Nodes / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D010835:Phytohemagglutinins / Q000494:pharmacology; D012513:Sarcoma, Experimental / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D003208:Concanavalin A