Characterization of a soluble suppressor of human B cell immunoglobulin biosynthesis produced by a continuous human suppressor T cell line.
A human suppressor T cell maintained in long-term culture with conditioned medium containing interleukin 2 elaborates a suppressor factor(s) that specifically inhibits human polyclonal B cell immunoglobulin biosynthesis. This soluble immune suppressor supernate of immunoglobulin production (CTC-SISS-B) shares a number of features with the previously described suppressive mediator elaborated by concanavalin A-activated human peripheral T cells (SISS-B) including: (a) the inhibition by a noncytotoxic mechanism, (b) the suppression of immunoglobulin biosynthesis either through direct action on the B cell or indirect action via the monocyte, (c) the loss of inhibition in the presence of the monosaccharide L-rhamnose, (d) the elaboration by cells irradiated with 500 ro 2,000 rad, and (e) molecular weights of 60,000--90,000. Furthermore, the suppression by this mediator appears to be specific for B cell immunoglobulin production in that CTC-SISS B has no effect on T cell proliferation to mitogens, antigens, an allogeneic cells or on T cell-mediated cytotoxicity. These data indicate that one possible mechanism of suppressor T cell inhibition of human immunoglobulin production is via the generation of a lectinlike suppressor lymphokine that interacts with defined saccharide determinants on the cell surface of either the B cell or monocyte.
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology*; D002850:Chromatography, Gel; D003470:Culture Media; D003602:Cytotoxicity, Immunologic; D005643:Fucose / Q000494:pharmacology; D005690:Galactose / Q000494:pharmacology; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D007700:Kinetics; D010835:Phytohemagglutinins / Q000494:pharmacology; D011817:Rabbits; D012210:Rhamnose / Q000494:pharmacology; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D000906:Antibodies; D000911:Antibodies, Monoclonal; D003470:Culture Media; D007136:Immunoglobulins; D010835:Phytohemagglutinins; D005643:Fucose; D012210:Rhamnose; D013936:Thymidine; D005690:Galactose