Molecular cloning of an immunoglobulin kappa constant gene from NZB mouse.
An EcoRI fragment carrying the immunoglobulin C kappa gene and multiple J gene segments from the DNA of the NZB strain of mouse was cloned into lambda Ch 4A DNA. Subsequent characterization of the clone by heteroduplex analysis, restriction-enzyme mapping and DNA sequencing demonstrated that the organization of the J gene segments and the C kappa gene of NZB mouse was similar, if not identical, to that of DNA from the Balb/c strain of mouse. Since the amino acid sequence of the light chain of a plasmacytoma of NZB mouse shows a J region sequence different from that of Balb/c mouse, our results indicate that the new J sequence arose by somatic mutation.
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D005796:Genes; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007128:Immunoglobulin Fragments / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D007136:Immunoglobulins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008814:Mice, Inbred NZB; D009154:Mutation; D009693:Nucleic Acid Hybridization
D007127:Immunoglobulin Constant Regions; D007128:Immunoglobulin Fragments; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D007136:Immunoglobulins