Immunohistochemical localization and distribution of S-100 proteins in the human lymphoreticular system.
Immunohistochemical localization of S-100b protein and S-100ao protein in human lymphoreticular system was studied by using monospecific antibody directed against either the alpha subunit or beta subunit of S-100 protein. S-100b protein immunoreactivity was detected in Langerhans cells, interdigitating reticulum cells, and histiocytosis X cells, but not in ordinary macrophages and blood monocytes. In contrast, S-100ao protein immunoreactivity was detected in blood monocytes, macrophages of lymph node, alveolar macrophages of lung, and small numbers of Kupffer cells of liver. S-100ao immunoreactivity was also detected in epithelioid cells, Langhans giant cells, and foreign body giant cells. The present findings suggest that the presence of S-100ao protein in the cytoplasm is one of the characteristic features of cells in the human mononuclear phagocyte system. The detection of S-100b, but not S-100ao, immunoreactivity in Langerhans cells and interdigitating reticulum cells also suggests that they are independent of the monocyte-macrophage system. S-100ao protein may be a novel cytoplasmic marker for cells of the human monocyte-macrophage system.
D015415:Biomarkers; D001996:Bronchopneumonia / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism; D006099:Granuloma / Q000378:metabolism; D006646:Histiocytosis, Langerhans-Cell / Q000378:metabolism; D006801:Humans; D007801:Langerhans Cells / Q000378:metabolism; D008199:Lymphadenitis / Q000378:metabolism; D008208:Lymphatic System / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism; D012157:Mononuclear Phagocyte System / Q000378:metabolism*; D009414:Nerve Growth Factors; D012156:Reticulocytes / Q000378:metabolism; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000378:metabolism*; D014388:Tuberculosis, Lymph Node / Q000378:metabolism
D015415:Biomarkers; D009414:Nerve Growth Factors; C030564:S-100 calcium-binding protein alpha subunit; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins