[Role of a disorder in poly(ADP-ribosylation) in the activation of Ca/Mg-dependent endonuclease].
The nuclei from the control and irradiated (3 h after irradiation at a dose of 10 Gy) thymocytes were preincubated with NAD in conditions optimal for poly (ADP) ribosylation. This was shown to decrease by 6-7- and 2-3 times, respectively, the rate of autolytic cleavage of DNA by Ca/Mg-dependent endonuclease. The inhibitors of poly (ADP-riboso)-polymerase, nicotine amide and thymidine, removed the effect of NAD. The data obtained prompt an assumption that the post-irradiation activation of Ca/Mg-nuclease in thymocytes is associated with the disturbance of its post-translation modification, poly(ADP)ribosylation.
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology / Q000528:radiation effects; D004247:DNA / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000378:metabolism* / Q000528:radiation effects; D004789:Enzyme Activation / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays; D008297:Male; D009243:NAD / Q000378:metabolism; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism* / Q000528:radiation effects; D011499:Protein Processing, Post-Translational / Q000187:drug effects / Q000528:radiation effects; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000201:enzymology / Q000528:radiation effects
D009702:Nucleoside Diphosphate Sugars; D009243:NAD; D011064:Poly Adenosine Diphosphate Ribose; D004247:DNA; D004706:Endodeoxyribonucleases; C029367:calcium magnesium dependent endodeoxyribonuclease
Rol' narusheniia poli(ADF-ribozilirovaniia) v aktivaktsii Ca/Mg-zavisimoĭ éndonukleazy.