[Purification and properties of copper thionein].
A novel procedure for purification of copper-thionein from rabbit liver was developed. The molecular mass, copper, and thiol contents were determined. The properties of the protein were compared with those of Cd-thionein. Copper was removed from Cu-thionein and the reconstitution was achieved. The binding of copper leads to an increase in absorbance at 260 nm.
D000818:Animals; D001059:Apoproteins / Q000032:analysis / Q000302:isolation & purification; D002850:Chromatography, Gel; D003300:Copper / Q000032:analysis; D008099:Liver / Q000032:analysis*; D008668:Metallothionein / Q000032:analysis / Q000302:isolation & purification*; D008970:Molecular Weight; D011817:Rabbits; D013054:Spectrophotometry, Atomic; D013438:Sulfhydryl Compounds / Q000032:analysis
D001059:Apoproteins; D013438:Sulfhydryl Compounds; C031834:apothionein; C004203:cadmium-binding protein; C007121:copper thionein; D003300:Copper; D008668:Metallothionein
Ochistka i svoĭstva med'-tioneina.