Guanyl nucleotides decrease antagonist binding at high affinity dopamine receptors.
Guanyl nucleotides induce a loss of high affinity [3H]spiroperidol binding sites in rat striatum that can be demonstrated by rate association, equilibrium dissociation and saturation analyses. [3H]Spiroperidol is an antagonist ligand for rat striatal dopamine receptors. These data therefore demonstrate clear cut effects of guanyl nucleotides on antagonist binding.
D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D003342:Corpus Striatum / Q000378:metabolism; D006150:Guanine Nucleotides / Q000494:pharmacology*; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D051381:Rats; D011954:Receptors, Dopamine / Q000187:drug effects*; D013134:Spiperone / Q000378:metabolism
D006150:Guanine Nucleotides; D011954:Receptors, Dopamine; D006165:Guanylyl Imidodiphosphate; D013134:Spiperone; D008274:Magnesium