In vitro inactivation of the neurotoxic action of beta-bungarotoxin by the marine natural product, manoalide.
The irreversible neurotoxic action of beta-bungarotoxin (beta-BuTx) can be prevented by preincubation of the toxin with manoalide, a non-steroidal anti-inflammatory agent. Manoalide was also found to inactivate purified phospholipase A2 and thus prevent hydrolysis of phosphatidylcholine. PLA2 is a component found in several neurotoxic venoms and is also a rate limiting enzyme important in phospholipid metabolism and prostaglandin synthesis in man.
D000818:Animals; D000893:Anti-Inflammatory Agents; D001681:Biological Assay; D002038:Bungarotoxins / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D003964:Diaphragm / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D010791:Phrenic Nerve / Q000502:physiology; D011161:Porifera / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D000893:Anti-Inflammatory Agents; D002038:Bungarotoxins; D010741:Phospholipases A; D054467:Phospholipases A2