Decrease of transport of some polyols in sickle cells.
This paper reports the results of kinetic studies on the inward net-flux of small non-electrolytes (ethylene glycol, glycerol and erythritol) in sickle cells as compared to normal erythrocytes. Net transport rates were evaluated by turbidimetric measurements for ethylene glycol and glycerol and by hematocrit monitoring for erythritol. A 2-fold and 4-fold reduction in the permeability coefficient for ethylene glycol and glycerol, respectively, were found in sickle cells as compared to normal erythrocytes. In contrast, no significant changes in erythritol transport kinetics were observed. The dependence of glycerol permeability on temperature, pH and oxygenation is the same in both types of cells. A significant correlation was observed between glycerol permeability and cell density only for sickle cells. The results indicate that irreversible modifications of membrane proteins, responsible for the glycerol and ethylene glycol transport, do occur in sickle cells.
D000755:Anemia, Sickle Cell / Q000503:physiopathology*; D001692:Biological Transport / Q000187:drug effects; D002463:Cell Membrane Permeability; D002729:Chloromercuribenzoates / Q000494:pharmacology; D004896:Erythritol / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000502:physiology*; D005026:Ethylene Glycols / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010100:Oxygen / Q000097:blood; D013696:Temperature
D002729:Chloromercuribenzoates; D005026:Ethylene Glycols; D005990:Glycerol; D004896:Erythritol; D010100:Oxygen