Hyperpolarizing and age-dependent depolarizing responses of cultured locus coeruleus neurons to noradrenaline.
The electrical activity and responses to noradrenaline (NA) of locus coeruleus (LC) neurons have been studied in organotypic cultures using intracellular recording. Most LC neurons were predominantly quiescent, though occasional bursts of activity were observed; a few cells were tonically active at rates of 0.5-5/s. In most cells tested, iontophoretic application of NA evoked responses which were initially hyperpolarizing, sometimes followed by a depolarizing phase and frequently followed by a period of increased excitatory synaptic activity. The enhanced synaptic activity appeared to be an indirect effect since it was blocked by bath application of tetrodotoxin (TTX). In the presence of TTX, responses to NA of all but one cell were simple hyperpolarizations or biphasic (hyperpolarization/depolarization) responses. The presence of the depolarizing component appeared to be age-dependent, since it was frequently observed in cultures grown in vitro for less than 26 days, while neurons in older cultures exhibited only hyperpolarizing responses. If such age-dependent depolarizing responses are present in vivo, they would represent a unique example of a transmitter response which is present only during a transient developmental phase.
D000818:Animals; D000831:Animals, Newborn; D002395:Catecholamines / Q000378:metabolism; D046508:Culture Techniques; D004553:Electric Conductivity; D004594:Electrophysiology; D008125:Locus Coeruleus / Q000187:drug effects* / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D009638:Norepinephrine / Q000494:pharmacology*; D009928:Organ Specificity; D013997:Time Factors
D002395:Catecholamines; D009638:Norepinephrine