Hydrolysis of cholesterol ester in artificial lipid mixtures by cholesterol esterase released from particulate fractions of rat arterial wall.
Acid and neutral cholesterol esterase activities in rat arterial wall were released from the lysosomal fraction and microsomal fraction respectively into the 105,000 X g supernatant fraction by treatment with Triton X-100, heparin and dextran sulfate. The percentage releases of acid cholesterol esterase by Triton X-100 (0.1%), heparin (50 micrograms/ml) and dextran sulfate (1 mg/ml) were 21%, 18% and 4%, respectively, while those of neutral cholesterol esterase were 66%, 56% and 39%, respectively. The cholesterol esterase released by dextran sulfate, especially that from the microsomal fraction, hydrolyzed cholesterol ester in artificial lipid mixtures with similar lipid compositions to those of the deposits in fatty streaks and fibrous plaques of atheromatous lesions.
D000818:Animals; D001158:Arteries / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002788:Cholesterol Esters / Q000378:metabolism*; D006868:Hydrolysis; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D008861:Microsomes / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D002787:Sterol Esterase / Q000378:metabolism*
D002788:Cholesterol Esters; D002265:Carboxylic Ester Hydrolases; D002787:Sterol Esterase