Characterization of an unusual isoenzyme of N-acetyl-beta-D-hexosaminidase from a human colonic carcinoma cell line.
A sub-line with increased metastatic ability was previously isolated from an established human colonic carcinoma cell line [Kimball & Brattain (1980) Cancer Res. 40, 1574-1579]. The separation and characterization of the isoenzymes of N-acetyl-beta-D-hexosaminidase from each cell line are reported. The parental cell line contained A and B isoenzymes. The sub-line lacked the A-isoenzyme activity and contained an atypical B isoenzyme that was thermolabile, susceptible to alkylation and of lower molecular weight.
D002460:Cell Line; D003110:Colonic Neoplasms / Q000201:enzymology*; D006596:Hexosaminidases / Q000302:isolation & purification; D006358:Hot Temperature; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000302:isolation & purification; D007700:Kinetics; D013849:Thimerosal / Q000494:pharmacology; D001619:beta-N-Acetylhexosaminidases
D007527:Isoenzymes; D013849:Thimerosal; D006596:Hexosaminidases; D001619:beta-N-Acetylhexosaminidases