Mitochondrial ribosome assembly in Neurospora crassa. Purification of the mitochondrially synthesized ribosomal protein, S-5.
In Neurospora, one mitochondrial ribosomal protein (S-5, Mr = 52,000) is synthesized intramitochondrially and is presumably encoded by mitochondrial DNA. We have developed a rapid method for the purification of S-5 which takes advantage of its high affinity for carboxymethyl-Sepharose in the presence of 6 M urea. Using this method, S-5, at purity greater than 95%, can be prepared by column chromatography in a single batch elution step. The amino acid composition of S-5 was determined. Judged by the contents of hydrophilic and basic amino acids, S-5 is more similar to Escherichia coli and yeast ribosomal proteins than to other mitochondrial translation products which are hydrophobic membrane proteins.
D000596:Amino Acids / Q000032:analysis; D004272:DNA, Mitochondrial / Q000235:genetics*; D046911:Macromolecular Substances; D008928:Mitochondria / Q000378:metabolism*; D008970:Molecular Weight; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000235:genetics / Q000302:isolation & purification*; D012270:Ribosomes / Q000378:metabolism*
D000596:Amino Acids; D004272:DNA, Mitochondrial; D046911:Macromolecular Substances; D012269:Ribosomal Proteins; C030831:ribosomal protein S5