Volume-regulatory K+ efflux during concentrative uptake of alanine in isolated rat hepatocytes.
Changes in cell volume and 42K+ efflux associated with concentrative alanine uptake were studied in isolated rat hepatocytes suspended in Krebs-Ringer bicarbonate buffer. After addition of 10 mM-alanine, cellular water volume increased by 15% and the rate constant of 42K+ efflux by 250%. Alanine-induced 42K+ efflux was abolished by quinine and was strongly decreased when the cell-volume increase was counteracted by sucrose. The results suggest that K+ efflux during alanine uptake is implicated in a volume-regulatory response.
D000409:Alanine / Q000378:metabolism*; D000818:Animals; D001030:Apamin / Q000494:pharmacology; D001834:Body Water / Q000378:metabolism; D005260:Female; D005665:Furosemide / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D011803:Quinine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013395:Sucrose / Q000494:pharmacology
D001030:Apamin; D013395:Sucrose; D005665:Furosemide; D011803:Quinine; D000409:Alanine; D011188:Potassium