Interaction of cytotoxic antibiotic dactylarin with glycolytic thiol enzymes in Ehrlich ascites carcinoma cells.
Cytotoxic effect of dactylarin on Ehrlich ascites carcinoma cells is caused by the inhibition of some SH-dependent glycolytic enzymes, especially of hexokinase (EC 2.7.1.1), glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) and 6-phosphofructokinase (EC 2.7.1.11). Dactylarin interacts with thiols, which explains its inhibitory effectiveness on the above glycolytic enzymes.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001572:Benzofurans / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007773:Lactates / Q000096:biosynthesis; D051379:Mice; D010732:Phosphofructokinase-1 / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001572:Benzofurans; D007773:Lactates; D013438:Sulfhydryl Compounds; C006170:dactylarin; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D006593:Hexokinase; D010732:Phosphofructokinase-1; D005947:Glucose