[Analysis of DNA degradation in the thymocytes of irradiated rats by flow cytometry].
The method of flow cytofluorometry of cells treated with probes specifically bound to AT- or GC-pairs of DNA was used to study DNA degradation in thymocytes of irradiated and hydrocortisone-treated rats. Death of thymocytes was shown to be accompanied by the decrease in the DNA content. The main regularities in the formation and accumulation of cells, the DNA content of which being lower than that of diploid cells, were the same as those of the internucleosome DNA fragmentation.
D000818:Animals; D001482:Base Composition / Q000187:drug effects / Q000528:radiation effects; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000032:analysis / Q000378:metabolism / Q000528:radiation effects*; D005434:Flow Cytometry; D005720:Gamma Rays; D006854:Hydrocortisone / Q000494:pharmacology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D013997:Time Factors
D004247:DNA; D003513:Cycloheximide; D006854:Hydrocortisone
Analiz degradatsii DNK v timotsitakh obluchennykh krys metodom protochnoĭ tsitometrii.