A method for monitoring ADP-induced thromboembolism in mice.
A method for monitoring ADP-induced thromboembolism in mice by means of the measurement of respiratory rate and depth was studied. The measurement were accurately carried out with a new method devised by us. In mice intravenously injected with 5-40 mg ADP/kg, it was found that the respiratory rate decreased transiently and dose-responsively, and that the platelet count also decreased transiently. On the other hand, no decrease in respiratory rate after administration of ADP was observed in neuraminidase-pretreated mice, in which the platelet count was significantly reduced. In the histological examination, platelet thrombi formed in the pulmonary vessels were observed 10 sec after administration of 10 and 40 mg/kg of ADP in mice which had not been treated with neuraminidase. The extent of thromboembolism at the dose of 40 mg/kg showed a tendency to be more marked than that at 10 mg/kg. Pretreatment with 300 mg dipyridamole/kg p.o. prevented the respiratory depression at 40 mg ADP/kg. However, with 10 mg dimorpholamine/kg i.v. or 30 mg warfarin/kg p.o., no preventive effect was observed. Therefore, it was concluded that it is possible to monitor ADP-induced thromboembolism in mice by means of the accurate measurement of the respiratory rate and depth.
D000244:Adenosine Diphosphate / Q000633:toxicity*; D000818:Animals; D004176:Dipyridamole / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D008168:Lung / Q000473:pathology; D008297:Male; D051379:Mice; D009025:Morpholines / Q000494:pharmacology; D009439:Neuraminidase / Q000494:pharmacology; D010976:Platelet Count; D011517:Prothrombin Time; D012119:Respiration / Q000187:drug effects; D013923:Thromboembolism / Q000139:chemically induced*; D014859:Warfarin / Q000494:pharmacology
D009025:Morpholines; D014859:Warfarin; D000244:Adenosine Diphosphate; D004176:Dipyridamole; C100074:dimorpholamine; D009439:Neuraminidase