Sodium glycolate absorption in rat intestine.
Absorption of sodium [1-14C]glycolate by rat intestine was studied by using the tissue accumulation technique with everted intestinal rings. Saturation kinetics was observed for the absorption of glycolate in the jejunoileal region, with a Km of 6.25 mM for glycolate and a Vmax of 5.56 mumole/30 min/g wet wt. The absorption was linear up to a period of 25 min at 37 degrees C. Jejunum and ileum showed significantly higher absorption of glycolate as compared to colon. Sulfhydryl binding agents, viz., p-chloromercuribenzoate and iodoacetate, and respiration inhibitors, e.g., KCN and 2,4-dinitrophenol, had no significant effect on glycolate uptake. However, glyoxylate and lactate showed significant inhibition at 6 mM concentration of the inhibitor. Pyridoxine deficiency had no effect on glycolate uptake by the rat intestine.
D019297:2,4-Dinitrophenol; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002264:Carboxylic Acids / Q000494:pharmacology; D002729:Chloromercuribenzoates / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D006016:Glycolates / Q000378:metabolism*; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000187:drug effects; D007461:Iodoacetates / Q000494:pharmacology; D019807:Iodoacetic Acid; D007700:Kinetics; D008297:Male; D011190:Potassium Cyanide / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D026681:Vitamin B 6 Deficiency / Q000378:metabolism*; D020245:p-Chloromercuribenzoic Acid
D002264:Carboxylic Acids; D002729:Chloromercuribenzoates; D004140:Dinitrophenols; D006016:Glycolates; D007461:Iodoacetates; C031149:glycolic acid; D020245:p-Chloromercuribenzoic Acid; D011190:Potassium Cyanide; D019297:2,4-Dinitrophenol; D019807:Iodoacetic Acid