Production of migration inhibition factor (MIF) by purified human T cell subpopulations.
T cells possessing suppressor activity (TG) were isolated from normal human peripheral blood by rosetting with IgG-coated ox red blood cells (OxRBC) as shown by their ability to inhibit pokeweed mitogen (PWM) induced proliferation and Ig synthesis in vitro. The suppressor activity of the intact T cell and Tg cell fractions was enhanced by concanavalin A (Con A) stimulation for 48 hr and supernatants from these Con A-treated cell cultures, after removal of mitogen, contained migration inhibition factor (MIF). Its release appeared to be primarily a function of the TG cells. The bulk of the MIF activity was present in a gel filtration fraction of mol. wt approx. 25,000, similar to that found in previous studies of Con A- or antigen-induced MIF in man.
D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis*; D008222:Lymphokines / Q000096:biosynthesis*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D007136:Immunoglobulins; D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D003208:Concanavalin A; D013936:Thymidine