[Validity of radioimmunoassay for blood and pituitary prolactin in the male rat. Effect of bromocriptine and thyrotropin-releasing hormone].
Validity of a radioimmunoassay for rat prolactin (PRL) in serum and pituitary is analysed in adult male rats. Data are presented bearing on the accuracy, precision and sensitivity of the method. Serum levels and pituitary content are respectively ranged from 2.56 to 28.03 ng PRL RP2 ml-1 and from 7.36 to 21.44 microgram PRL RP2 per gland in intact animals. Treatment with bromocriptine (10 days) results in a decrease of serum PRL levels and pituitary PRL contents. In progesterone-estradiol benzoate pretreated rats, serum PRL levels are increased 20 min after the injection of TRH.
D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology*; D008297:Male; D010903:Pituitary Gland, Anterior / Q000032:analysis*; D011388:Prolactin / Q000032:analysis* / Q000097:blood; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology*
D001971:Bromocriptine; D013973:Thyrotropin-Releasing Hormone; D011388:Prolactin
Etude de la validité du dosage radioimmunologique de la prolactine sérique et hypophysaire chez le rat mâle. Action de la bromocriptine et de la thyréolibérine (TRH).