[Biosynthesis of protein and RNA during changes in the Na and K ratios in Ehrlich carcinoma cells].
The changes in the intracellular ratios of Na+ and K+ by equilibration of Ehrlich carcinoma cells in salt media at 0-4 degrees C leads to the changes in the rate of rRNA and protein synthesis. Using competitive hydridization, it was shown that with a decrease of the Na+/K+ ratio from 1:1 to 1:2, the rate of pre-rRNA synthesis is markedly diminished. The incorporation of labelled amino acids into histones, nonhistone proteins, and cytoplasmic proteins also depends on the ratio of monovalent cations in cancer cells. At the translation level, Na+ and K+ are responsible for the selectivity of protein synthesis, whereas upon transcription these cations determine the rate of synthesis of corresponding mRNAs. It is concluded that the intracellular ratio of monovalent cations is one of the mechanisms, by means of which the cells operate their genetic control.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis; D006657:Histones / Q000096:biosynthesis; D007424:Intracellular Fluid / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D011188:Potassium / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012964:Sodium / Q000378:metabolism*
D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal; D012964:Sodium; D011188:Potassium
Biosintez belka i RNK pri izmenenii sootnosheniia Na+ i K+ v kletakh raka Erlikha.