The Na+-ionophore monensin enhances glucose uptake in mouse thymocytes.
Monensin enhanced 2-deoxyglucose uptake and 3-O-methyl glucose transport in mouse thymocytes, but had no effect on L-glucose transport. Cytochalasin B inhibited monensin induced as well as basal glucose uptake. The enhanced 2-deoxyglucose uptake was time and dose-dependent. The increase in the rate of 2-deoxyglucose uptake induced by monensin was more rapid than that of Na+ uptake. Ouabain did not inhibit monensin-enhanced 2-deoxyglucose uptake. Monensin failed to stimulate 2-deoxyglucose uptake at low concentrations of Na+ (13 mM) or K+ (17 mM), higher concentrations of either cation were required for stimulation. Monensin enhanced glucose uptake also in Ca2+-free medium. The data indicate that the stimulation of 2-deoxyglucose uptake by monensin results from activation of carrier-mediated transport.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D003571:Cytochalasin B / Q000494:pharmacology; D005260:Female; D005663:Furans / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008985:Monensin / Q000494:pharmacology*; D013950:Thymus Gland / Q000378:metabolism
D005663:Furans; D003571:Cytochalasin B; D008985:Monensin; D005947:Glucose