[Biosynthesis of pulmonary glycoconjugates. Mechanism of glucosylation of protein and lipid acceptors].
In sheep lung microsomes, we have shown that glucosyl-transferases catalize the transfer of glucose from UDP-glucose into four different acceptors. The glucosylated products obtained are as follows: - a glucosyl-phosphoryl-polyprenol (product A) extractable by chloroform/methanol (2:1 by volume). - a product B extractable by chloroform/methanol/water (10:10:3 by volume). The product B was a mixture of five glycolipids, one of them having a chromatographic behaviour similar to the behaviour of a tetrahexosylceramide (asia-lo-GM1). - a product C insoluble in water and organic solvents which has been demonstrated to be a glycoproteinic compound. The molecular weight of this product C was 160 000 as estimated by gel-filtration. The carbohydrate moiety is composed of small oligosaccharides which are found to be attached by O-glycosidic bond to the protein chain. This linkage is not a collagen-like bond.
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D005964:Glucosyltransferases / Q000378:metabolism*; D006017:Glycolipids / Q000096:biosynthesis*; D006023:Glycoproteins / Q000096:biosynthesis*; D007700:Kinetics; D008168:Lung / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology*; D011106:Polyisoprenyl Phosphates / Q000378:metabolism; D012756:Sheep; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
D006017:Glycolipids; D006023:Glycoproteins; D011106:Polyisoprenyl Phosphates; C011082:polyprenyl monophosphate; D005964:Glucosyltransferases; D014532:Uridine Diphosphate Glucose
Biosynthèse des glycoconjugués pulmonaires. IV. Mécanisme de la glucosylation des accepteurs protéinique et lipidique.