"Self-reactive" T cells. II. Transferred syngeneic lymphoblasts induce T "stimulator" cells in vivo, to which syngeneic T cells "respond" in a syngeneic MLC in vitro.
Splenic T cells, engaged in an in-vivo "response" to transferred syngeneic lymphoblasts, displayed an extensive proliferative activity if cultured in vitro. These cells (blocked by mitomycin C) were "stimulator" cells in a one-way syngeneic mixed lymphocyte culture (S-MLC). "Responder" cells were derived form spleens of syngeneic (sex and age matched) mice. Fractionation studies showed that "stimulator" and "responder" cells in the investigated S-MLC were nylon-wool non-adherent and sensitive to treatment with anti-Thy-1 antiserum and complement, i.e. were cells of the T lineage. Coculture experiments demonstrated that direct cell contact is required to trigger a proliferative "response" in the S-MLC. Fetal calf serum-supplemented tissue-culture medium supported "responder" cell proliferation, normal mouse serum-supplemented medium did not. The in-vivo generation of a T-cell population, which could "stimulate" a syngeneic T-cell "response" in the S-MLC in vitro, was investigated.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002469:Cell Separation; D003165:Complement System Proteins; D005260:Female; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D000961:Antilymphocyte Serum; D003165:Complement System Proteins