The relationship between polyribosomal and latent membrane-bound messenger ribonucleoprotein particles in Artemia embryos.
Polysomal messenger ribonucleoprotein (mRNP) particles from developing Artemia cysts were isolated, characterized and compared with latent membrane-bound mRNP particles isolated from dormant cysts. The polyribosomal mRNP particles sedimented between 25-35 S in a sucrose gradient and had a buoyant density of 1.33 g/cm3 in Cs2So4. Latent particles had a higher sedimentation coefficient and lower buoyant density. The poly(A) + RNA in the two kinds of particles was comparable in size, 10-20 S. The protein composition of the particles, as determined by electrophoresis, was different. Polyribosomal particles contained 9 major and 6 minor proteins; a 72 k poly(A)-associated protein was present. Latent particles were characterized by a complex protein pattern ranging in apparent mol. wt between 14,000-140,000. Some proteins with similar molecular weight and isoelectric point were probably common to both kinds of particles.
D000818:Animals; D001156:Artemia / Q000502:physiology*; D005786:Gene Expression Regulation; D007425:Intracellular Membranes / Q000378:metabolism; D007526:Isoelectric Point; D008970:Molecular Weight; D011061:Poly A / Q000032:analysis; D011132:Polyribosomes / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*
D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein; D011061:Poly A