Chromatographic characterization of in vitro metabolites of 5-[2-(N,N-dimethylamino)ethoxy]-7-oxo-7H-benzo[c]fluorene.
Detection reactions and RF values in thin-layer chromatography on silica gel were studied for the antineoplastic drug Ih (benfluron) and related substances. On incubation of Ih with homogenate fractions of mammalian livers the N-oxide Ii and 5-[2-(N,N-dimethylamino)ethoxy]-7-hydroxy-7 H-benzo[c]fluorene (IIh) were established as products, and 5-[2-(N-methylamino)ethoxy]-7-oxo-7 H-benzo[c]fluorene (Ig), 5-[2-(N-methylamino)ethoxy]-7-hydroxy-7 H-benzo[c]fluorene (IIg) and a phenolic product of benfluron (IV) were tentatively identified.
D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer / Q000379:methods; D005449:Fluorenes / Q000032:analysis* / Q000378:metabolism; D007202:Indicators and Reagents; D008099:Liver / Q000032:analysis / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D009243:NAD / Q000378:metabolism; D009602:Nitrosamines / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D000970:Antineoplastic Agents; D005449:Fluorenes; D007202:Indicators and Reagents; D009602:Nitrosamines; D011506:Proteins; D009243:NAD; C034234:VUFB 13468; C041199:N-nitrosoallyl-2,3-dihydroxypropylamine