[Studies on the suppressor cells acting on the mitogen induced blastogenesis in tumor-bearing hosts. II. Suppressor cells in the peripheral blood of patients with malignant bone and soft tissue sarcoma (author's transl)].
The responses to Concanavalin A (Con A) of peripheral blood mononuclear cells were studied in 22 patients with malignant bone and soft tissue sarcoma. The decreases of response were shown in many cases depending on their clinical stages. The responses were increased by removal of the adherent cells from mononuclear cells by passing through a Sephadex G-10 column in 7 of 13 cases tested. The enhancement of responses to Con A was also found when indomethacin, a inhibitor of prostaglandin biosynthesis, was added to mononuclear cell suspensions. However, no additional increase of the responses was found when indomethacin was supplemented to the adherent cell-depleted mononuclear cell suspensions. Since it has been previously shown that prostaglandin was primarily produced from macrophages, the author concluded that the suppressor cells affecting on the lymphocyte transformation probably belong to monocyte-macrophage series and their suppressive effect may be mediated by prostaglandin. In the normal control, the responses of mononuclear cells to Con A stimulation were rather reduced by removal of the adherent cells, suggesting that these suppressor cells were contained only in mononuclear cells of the tumor-bearing hosts.
D001859:Bone Neoplasms / Q000097:blood / Q000276:immunology*; D002448:Cell Adhesion; D003208:Concanavalin A / Q000494:pharmacology*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D009000:Monocytes / Q000276:immunology; D012509:Sarcoma / Q000097:blood / Q000276:immunology*; D012983:Soft Tissue Neoplasms / Q000097:blood / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology*
D003208:Concanavalin A; D007213:Indomethacin