Effect of recombinant DNA-produced bovine interferon alpha (BoIFN-alpha 1) on the interaction between bovine alveolar macrophages and bovine herpesvirus type 1.
Treatment of bovine alveolar macrophages (BAM) with bovine leukocyte interferon (BoIFN-alpha 1) resulted in reduced bovine herpesvirus type 1 replication and spread. This was demonstrated by reduced virus yields and number of cells infected. BoIFN-alpha 1 treatment of BAM also induced enhanced Fc receptor expression and/or avidity by the cells, increased their activity in antibody-dependent cellular cytotoxicity, and augmented their extrinsic antiviral activity as measured by a reduction in the development of plaques in a susceptible cell line. These results are discussed in the context of the possible role of interferon in activation of AM during the early phases of a virus infection.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D002417:Cattle; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D004198:Disease Susceptibility; D004912:Erythrocytes / Q000276:immunology; D006564:Herpesviridae / Q000187:drug effects / Q000502:physiology*; D007370:Interferon Type I / Q000494:pharmacology / Q000502:physiology*; D007668:Kidney; D008264:Macrophages / Q000502:physiology*; D011961:Receptors, Fc / Q000032:analysis; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects
D007370:Interferon Type I; D011961:Receptors, Fc