Transfer of Salmonella typhimurium and Klebsiella pneumoniae genes in E. coli K12 by mini-muduction.
Using Mu/mini-Mu mixed lysates prepared by induction of Salmonella typhimurium and Klebsiella pneumoniae lysogenic for Mucts62 or the Mu-P1 hybrid, MPh1, and mini-Mu, Mu18A-1, we were able to transfer Arg and His genes from Klebsiella and Salmonella into E. coli K12.
D001120:Arginine / Q000235:genetics; D010583:Bacteriophage mu / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D006639:Histidine / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics*; D014161:Transduction, Genetic
D006639:Histidine; D001120:Arginine