Human placenta beta-galactoside-binding lectin. Purification and some properties.
A beta-galactoside-binding lectin was extracted from human placenta homogenate with lactose solution and purified to apparent homogeneity by affinity chromatography on asialofetuin-Sepharose. The apparent subunit molecular weight of the lectin was 13,800 and its isoelectric point was about 5. Several saccharides containing D-galactose inhibited the hemagglutinating activity. The lectin resembles other vertebrate beta-galactoside-binding lectins in various biochemical characteristics.
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates; D002846:Chromatography, Affinity / Q000379:methods; D005260:Female; D005690:Galactose; D037161:Galectins; D006384:Hemagglutination; D006801:Humans; D037102:Lectins / Q000302:isolation & purification*; D046911:Macromolecular Substances; D008970:Molecular Weight; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy
D000596:Amino Acids; D002241:Carbohydrates; D037161:Galectins; D037102:Lectins; D046911:Macromolecular Substances; D005690:Galactose