Physical interaction between the phage lambda receptor protein and the carrier-immobilized maltose-binding protein of Escherichia coli.
When Triton X-100/EDTA extracts of the outer membrane of Escherichia coli K12 were passed through a column containing maltose-binding protein covalently linked to Sepharose 6MB beads, the phage lambda receptor protein or LamB protein was quantitatively and specifically adsorbed to the column and was eluted with a solution containing 1 M NaCl, but not with that containing 0.5 M maltose. The binding did not take place when columns containing inactivated Sepharose beads alone, or Sepharose bound to histidine-binding protein of Salmonella typhimurium, were used. This interaction is consistent with the hypothesis that the periplasmic maltose-binding protein interacts with the part of the LamB protein exposed on the inner surface of the outer membrane, thereby increasing the specificity of the solute penetration process through the LamB channel.
D018528:ATP-Binding Cassette Transporters; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000378:metabolism*; D010582:Bacteriophage lambda / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002846:Chromatography, Affinity; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D058619:Maltose-Binding Proteins; D008565:Membrane Proteins / Q000378:metabolism; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; D018272:Porins; D011485:Protein Binding; D011991:Receptors, Virus / Q000302:isolation & purification / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C461619:MalE protein, E coli; D058619:Maltose-Binding Proteins; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; D018272:Porins; D011991:Receptors, Virus; C018963:histidine-binding protein; C016532:maltoporins; C461599:maltose transport system, E coli