Reaction heats and heat capacity changes for intermediate steps of the ATP hydrolysis catalyzed by myosin subfragment 1.
The interaction of myosin Subfragment 1 with ATP in 0.1 M KCl containing 0.01 M MgCl2 and 0.02 M Tris/HCl (pH 8.0) was studied by microcalorimetry at temperatures of 4, 12, and 23 degrees C so that values of the heat capacity change (delta Cp) could be obtained for intermediate steps of the ATPase cycle. The delta Cp values are large compared to the value for the overall cycle, indicating that large changes in the hydrophobic effect are involved in transitions between different intermediate states. However, the heat capacity changes themselves show peculiar temperature dependences. Thus bindings of ATP and ADP to Subfragment 1, both of which are strongly exothermic processes, take place with large negative delta Cp of about -3 kJK-1 mol-1 between 4 and 12 degrees C but with very small delta Cp of 0.3-0.4 kJ K-1 mol-1 between 12 and 23 degrees C. On the contrary, the delta Cp for the endothermic hydrolysis of ATP bound to Subfragment 1 is positive (congruent to kJK-1 mol-1) in the lower temperature range but strongly negative (congruent to -4 kJK-1 mol-1) in the higher temperature range. The magnitude of delta Cp for the slow Pi dissociation process is similar but its sign is just opposite to that for the hydrolysis. These anomalous changes in the heat capacity may be due to the temperature-induced changes in a balance between large opposing effects which result from distinct, local conformation changes within the Subfragment 1 molecule.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002151:Calorimetry; D006358:Hot Temperature; D007700:Kinetics; D009132:Muscles / Q000201:enzymology; D015879:Myosin Subfragments; D009218:Myosins / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D011817:Rabbits
D015879:Myosin Subfragments; D010446:Peptide Fragments; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D009218:Myosins