Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes.
Fragments from section 3 of the salivary gland X chromosome of D. melanogaster were dissected with a micromanipulator. The DNA was extracted, cut and ligated to a lambda vector in a volume of a few nanoliters in an oil chamber monitored through a microscope. From about 10 pg of DNA we obtained 80 recombinant clones, a sample of which were analysed and shown to contain Drosophila DNA which hybridises in situ to the region of section 3 of the X chromosome. With this technique we can isolate clones from any desired region as small as 200 kb from the euchromatic arms of polytene chromosomes.
D010582:Bacteriophage lambda / Q000235:genetics; D002875:Chromosomes / Q000648:ultrastructure*; D003001:Cloning, Molecular / Q000379:methods; D004331:Drosophila melanogaster / Q000235:genetics*; D049711:History, Modern 1601-; D008846:Micromanipulation; D009693:Nucleic Acid Hybridization; D012469:Salivary Glands / Q000648:ultrastructure