Effect of Mg2+ and Ca2+ on enzymatic elastolysis of insoluble elastin determined by a conductimetric method.
The effect of some divalent cations on the elastolysis of insoluble elastin by pancreatic elastase has been studied with a conductimetric method. Mn2+ and Cu2+ give progressive inhibition, while Ca2+ initially slightly increases elastolysis, then becomes an inhibitor at concentrations higher than 10(-4) M. A stronger effect is demonstrated with Mg2+ which increases the initial velocity of the enzymatic reaction. However, there is no effect when N-succinyl-(Ala)(3)-paranitroanilide is used as the substrate. The different effects observed can be explained by an enzymatic activation process (cation-enzyme complex) and/or by a conformational change of the insoluble elastin.
D000813:Anilides / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002417:Cattle; D003300:Copper / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004549:Elastin / Q000378:metabolism*; D004553:Electric Conductivity; D006868:Hydrolysis; D008274:Magnesium / Q000494:pharmacology*; D008345:Manganese / Q000494:pharmacology; D009842:Oligopeptides / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism*
D000813:Anilides; D009842:Oligopeptides; D008345:Manganese; C015516:succinyl-trialanine-4-nitroanilide; D003300:Copper; D004549:Elastin; D010196:Pancreatic Elastase; D008274:Magnesium; D002118:Calcium