Cleavage of C3 by neutral proteases from granulocytes in pleural empyema.
The possibility of direct inactivation of C3 by granular enzymes from polymorphonuclear leukocytes (PMNLs) in pleural empyema was examined. As a group, pleural empyema from 10 patients with purulent effusions and a positive bacteriologic culture cleaved significantly more 125I-labeled C3 bound to Sepharose (18.4% +/- 7.3%) than did 19 sterile pleural effusions (2.4% +/- 0.9%; P less than 0.001) and sonicates from bacterial strains commonly found in empyema (1.4% +/- 0.2%). Granular enzymes from 7 X 10(6) PMNLs cleaved 78.5% of 125I-labeled C3 bound to Sepharose. When proteolysis of 125I-labeled C3 after incubation with pleural empyema or PMNL granular enzymes was examined with polyacrylamide gel electrophoresis, breakdown products were similar. Granulocyte elastase-like activity was detected in four samples of pleural empyema. Granulocyte elastase inhibitors, as well as 10% human serum, effectively suppressed cleavage of C3 and elastase-like activity. In pleural empyemas, granula enzymes from PMNLs, especially elastase, apparently contribute to low complement-mediated opsonic activity by direct inactivation of C3.
D000328:Adult; D000368:Aged; D003176:Complement C3 / Q000378:metabolism*; D003180:Complement C3b Inactivator Proteins / Q000378:metabolism; D015933:Complement C3d; D017242:Complement Factor H; D004492:Edetic Acid / Q000494:pharmacology; D004653:Empyema / Q000201:enzymology / Q000276:immunology*; D005260:Female; D006098:Granulocytes / Q000201:enzymology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology; D010447:Peptide Hydrolases / Q000494:pharmacology*; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D010996:Pleural Effusion / Q000201:enzymology; D011480:Protease Inhibitors; D012685:Sepharose / Q000378:metabolism
C493495:CFH protein, human; D003176:Complement C3; D003180:Complement C3b Inactivator Proteins; D011480:Protease Inhibitors; D010664:Phenylmethylsulfonyl Fluoride; D015933:Complement C3d; D017242:Complement Factor H; D012685:Sepharose; D004492:Edetic Acid; D010447:Peptide Hydrolases