[Ca2+-dependent ATPases of the sarcoplasmic reticulum of skeletal and cardiac muscles and their ion-transporting fragments].
The data are presented on fractionation of cardiac and skeletal muscle Ca2+-ATPase fragments obtained by trypsin and cyanobromide hydrolysis. Amino acid composition of Ca2+-ATPases isolated from the heart and skeletal muscles and of enzyme fragments is determined. The ionophoric properties of the intact enzyme and its fragments are studied. The low-molecular weight enzyme fragment decreases velocity and the level of Ca2+-accumulation by the vesicles of sarcoplasmic reticulum. It is suggested that an "ionphoric" fragment of muscle ATPase is able to form transmembrane Ca2+-selective channel in model lipid membranes.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D010856:Columbidae; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*
D000596:Amino Acids; D000252:Calcium-Transporting ATPases; D002118:Calcium
Issledovanie Ca2+-zavisimykh ATF-az sarkoplazmaticheskogo retikuluma skeletnoĭ i serdechnoĭ myshts i ikh iontransportiruiushchikh fragmentov.