Purification of creatine kinase-BB isoenzymes from human and canine tissues.
We describe a procedure for the purification of creatine kinase BB isoenzyme from canine and human brain tissue. The purification involves sequentially 50-70% (NH4)2SO4 fractionation, DEAE-Sephacel, Blue-Sepharose CL-6B, and chromatofocusing chromatography. This method produces a homogeneous protein purification as verified by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing chromatography.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003402:Creatine Kinase / Q000302:isolation & purification*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes; D011817:Rabbits
D007527:Isoenzymes; D003402:Creatine Kinase