[Cytology and phospholipid content of the bronchoalveolar lavage liquid in diffuse interstitial pneumopathies and in sarcoidosis. Apropos of 96 lavages].
The phospholipid content of bronchoalveolar lavage (LBA) has been little studied till now. This work involved 96 LBA on 7 cases of allergic alveolitis, 11 cases of diffuse interstitial fibrosis (FID) and 42 of sarcoidosis. The liquid collected was submitted to a cellular study (cellularity, total and differential) and biochemical (total proteins, total phospholipids separated into their different fractions by chromatography which enabled the identification of phosphatidyl-choline, phosphatidyl-serine and phosphatidyl-inositol in particular). The cytological results were close to the classical data, a rise in the lymphocyte level in allergic alveolitis and active sarcoid, a rise in polymorphonuclear cells in FID. Biochemical analysis showed a fall in total phospholipids for the whole group, the drop being the greatest in the FID. Phosphatidyl-choline, the principle phospholipid of surfactant in normals, was reduced in all three groups of patient when expressed as a percentage of total phospholipids. This fall was greatest with an allergic alveolitis and occurred on a pro rata basis with a rise in phosphatidyl-serine and inositol. The protein phospholipid ratio (PL/P) expressed in micrograms of phospholipid and milligrams of protein fell in a significant fashion in all the pathological groups. This fall was significantly larger in allergic alveolitis than FID and active sarcoid. The PL/P ratio seems to be a good marker of active sarcoid, correlating with the activity of the disease inversely with the lymphocyte level, and was unchanged by steroids unlike the lymphocyte count. A rise in this ratio correlates with an improvement in the radiological and clinical state.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001980:Bronchi / Q000473:pathology*; D002452:Cell Count; D005260:Female; D006801:Humans; D008171:Lung Diseases / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010743:Phospholipids / Q000032:analysis*; D011650:Pulmonary Alveoli / Q000473:pathology*; D011658:Pulmonary Fibrosis / Q000473:pathology*; D012507:Sarcoidosis / Q000473:pathology*; D007507:Therapeutic Irrigation
D010743:Phospholipids
Etude cytologique et phospholipidique du liquide de lavage broncho-alvéolaire au cours des pneumopathies interstitielles diffuses et des sarcoïdoses. A propos de 96 lavages.