Carbon tetrachloride-dependent inhibition of lipid secretion by isolated hepatocytes. Characterization and requirement for bioactivation.
Secretion of lipid as very low density lipoprotein (VLDL) by isolated hepatocytes was studied in a system in which the cells were exposed to a constant concentration of carbon tetrachloride (CCl4) throughout the duration of the incubation. Inhibition of secretion was characterized in terms of CCl4 concentration and duration of incubation. Half-maximum inhibition of VLDL secretion occurred at 80 microM CCl4. At 390 microM CCl4, VLDL secretion was inhibited 40% in 2 min and was suppressed completely in 5 min. No CCl4-dependent release of cellular glutamic oxaloacetic transaminase occurred under the conditions studied. Evidence is presented indicating that the metabolism of CCl4 is required for the expression of CCl4-dependent inhibition of lipid secretion. With respect to the parameters studied, the isolated hepatocyte system closely mimics the hepatic response of the intact animal to CCl4.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001711:Biotransformation; D002251:Carbon Tetrachloride / Q000378:metabolism; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism*; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008079:Lipoproteins, VLDL / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D008079:Lipoproteins, VLDL; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases