Urinary excretion of xanthurenic acid and zinc in diabetes: 1) Separation of xanthurenic acid-Zn2+ complex by ion-exchange chromatography.
In view of the relevance of urinary xanthurenic acid (XA) and zinc in diabetes, a chromatographic procedure has been devised which allows separation of XA from its complex with Zn2+. The procedure involves chromatography on DEAE-Sepharose CL-6B at pH 7.2 with a stepwise Tris-HCl concentration gradient. The ultraviolet absorption difference spectra due to formation of the XA-Zn2+ complex allowed the estimate of the binding molar ratio in the XA-Zn2+ complex. This corresponds to a 1:1 as estimated on the basis of the Hill plot of the experimental data.
D002852:Chromatography, Ion Exchange; D003920:Diabetes Mellitus / Q000652:urine*; D006801:Humans; D007736:Kynurenic Acid / Q000031:analogs & derivatives* / Q000652:urine; D013056:Spectrophotometry, Ultraviolet; D014977:Xanthurenates; D015032:Zinc / Q000652:urine*
D014977:Xanthurenates; C028330:xanthurenic acid; D007736:Kynurenic Acid; D015032:Zinc