An improved radioimmunoassay for human ovarian tumor antigen NB/70K.
An improved assay for the measurement of human ovarian tumor antigen NB/70K (triton NB/70K assay) has been developed. This assay makes the accurate and reproducible estimation of serum NB/70K possible. Freezing and thawing of serum up to three times or refrigeration of diluted serum up to 7 days does not significantly alter the NB/70K levels detected by the triton NB/70K assay. NB/70K levels in different volumes of the same sample showed a linear relationship to volume, with a correlation coefficient usually above 0.98.
D000951:Antigens, Neoplasm / Q000032:analysis*; D001793:Blood Preservation; D005069:Evaluation Studies as Topic; D005260:Female; D005615:Freezing; D006801:Humans; D010051:Ovarian Neoplasms / Q000276:immunology*; D011863:Radioimmunoassay / Q000379:methods
D000951:Antigens, Neoplasm; C017589:ovarian tumor associated antigen