[Biphase reaction of enzymatic pyruvate reduction during inhibition].
At least two stages have been revealed in the reaction of enzymatic reduction of pyruvate under inhibition. A fast process during the first stage of the reaction was finished in a period of "dead time" with routine spectroscopic measurements. A reaction rate at this stage decreased by three times. The second stage was characterized by a constant rate, which changed by less than 10%. The analysis of neutral salt influences on the inhibition complex showed, that the latter decomposed rapidly. A model suggested earlier, was used for interpreting the reversible product inhibition of the reaction catalysed by LDH.
D000818:Animals; D066298:In Vitro Techniques; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009132:Muscles / Q000201:enzymology; D010084:Oxidation-Reduction; D011773:Pyruvates / Q000378:metabolism*; D013379:Substrate Specificity; D013552:Swine
D011773:Pyruvates; D007770:L-Lactate Dehydrogenase
Bifaznost' reaktsii fermentativnogo vosstanovleniia piruvata v usloviiakh ingibirovaniia.