Effect of antitubular basement membrane and brush border antibodies on p-aminohippurate transport in kidney.
The effect of antiproximal tubule basement membrane (TBM) and brush border (BB) antibodies on p-aminohippurate (PAH) transport was evaluated in a rat model of immunologically mediated interstitial nephritis. Immunized rats developed anti-TBM antibody titers ranging from 1 to 3,072 with continuous linear IgG and interrupted C3 deposits in the TBM. Circulating anti-BB antibodies were detected in half of the immunized rats in titers ranging from 1 to 128. Heavy IgG deposition was present in the BB when circulating anti-BB antibody titers exceeded eight and proteinuria was present. When anti-TBM antibody titers were 1,024 or greater the slice-to-medium PAH concentration ratio (S/M) was significantly reduced (p less than 0.001). Combined immunofluorescent microscopy and section freeze-dry autoradiography revealed normal cellular distribution of PAH-3H in all proximal tubules except in rat microscopic foci of interstitial nephritis in which concentrative transport was absent. However, luminal secretion of PAH-3H was strikingly reduced in tubules with heavy IgG BB deposits. 3-hour PAH secretion in vivo fell significantly in rats with circulating and tissue anti-BB antibodies. Antibody inhibition of PAH transport appeared to be independent of morphologic damage.


CONCLUSIONS
Anti-TBM antibodies were associated with decreased slice uptake of PAH-3H while anti-BB antibodies were associated with decreased luminal PAH secretion in vitro and in vivo.
D000618:Aminohippuric Acids / Q000378:metabolism*; D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D001485:Basement Membrane / Q000276:immunology; D001666:Binding Sites, Antibody; D001692:Biological Transport; D005260:Female; D005455:Fluorescent Antibody Technique; D007105:Immune Complex Diseases / Q000276:immunology* / Q000503:physiopathology; D007074:Immunoglobulin G / Q000276:immunology; D007684:Kidney Tubules / Q000276:immunology; D008871:Microvilli / Q000276:immunology; D009395:Nephritis, Interstitial / Q000276:immunology* / Q000503:physiopathology; D051381:Rats; D010130:p-Aminohippuric Acid / Q000378:metabolism*
D000618:Aminohippuric Acids; D001323:Autoantibodies; D007074:Immunoglobulin G; D010130:p-Aminohippuric Acid