Protein composition of adenovirus nucleoprotein complexes extracted from infected cells.
A viral nucleoprotein complex was extracted from the nuclei of human cells 20 hr after infection with adenovirus type 2 or several of its temperature-sensitive mutants. In its sedimentation property, density in CsCl, and digestion pattern with micrococcal nuclease, the complex resembled viral cores. The polypeptides V, PVII, 11K, and 36K were found associated with this complex which is formed prior to or in the absence of virus assembly. The results suggest that this nucleoprotein complex is a direct precursor to virus assembly.
D000260:Adenoviruses, Human / Q000235:genetics*; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D002467:Cell Nucleus; D002472:Cell Transformation, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008836:Micrococcal Nuclease; D009154:Mutation; D009698:Nucleoproteins / Q000302:isolation & purification*; D013696:Temperature; D014764:Viral Proteins / Q000302:isolation & purification*
D009698:Nucleoproteins; D014764:Viral Proteins; D008836:Micrococcal Nuclease