The effect of Ca+ free medium on the two different tricarboxylate cycles in the rat brain cortex slices.
The authors studied the effect of calcium-free saline-glucose medium on labelled amino acid and [14C]CO2 synthesis from U-[14C]-glucose and 1-[14C]-acetate in rat brain cortex slices. Respiration was stimulated parallel with increased synthesis of labelled amino acids and of [14C]CO2 from glucose to [to 150 % and 170 % respectively]. Specific glutamate, aspartate and neutral amino acid activity rose, GABA and glutamine were unaffected. Amino acid synthesis from 1-[14C]-acetate was inhibited [58%], while [14C]CO2 release was unchanged. This indicates that oxygen consumption via the tricarboxylase cycle associated with the small glutamate pool [and probably localized in the glia cells] did not alter in calcium-free medium, although conversion to amino acids was impaired.
D000085:Acetates / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002245:Carbon Dioxide / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D002952:Citric Acid Cycle / Q000187:drug effects*; D005260:Female; D005947:Glucose / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats
D000085:Acetates; D000596:Amino Acids; D005971:Glutamates; D005973:Glutamine; D002245:Carbon Dioxide; D005947:Glucose; D002118:Calcium