An automatic method for determination of urinary and myofibrillar 3-methylhistidine: fractional rate of myofibrillar protein breakdown in rats fed on casein as source of protein.
An automatic, more rapid and simplified analytical system for determination of 3-methylhistidine in urine and skeletal muscle is described, which may be applied to more extensive studies of large number of samples within a reasonable period of time and constitutes a powerful tool in understanding the dynamics of protein metabolism in the intact organism. This procedure allows the analysis of 3-Methylhistidine by ion-exchange chromatography in 140 +/- 2.5 min using a single column system. The mean urinary 3-Methylhistidine output of rats weighing about 200 g fed on an adequate diet of casein was 0.84 +/- 0.02 microM/100 g BW, and the mean values for skeletal muscle in these animals were 0.74 +/- 0.03 microM/g tissue. The fractional rate of myofibrillar protein breakdown assessed by the urinary 3-Methylhistidine was 0.028 +/- 0.002%.
D000818:Animals; D002364:Caseins / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D004044:Dietary Proteins / Q000378:metabolism*; D006639:Histidine / Q000031:analogs & derivatives*; D008297:Male; D008762:Methylhistidines / Q000032:analysis* / Q000652:urine; D009124:Muscle Proteins / Q000378:metabolism*; D009210:Myofibrils / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D002364:Caseins; D004044:Dietary Proteins; D008762:Methylhistidines; D009124:Muscle Proteins; D006639:Histidine; C028118:3-methylhistidine