[Selective modification of polyadenyl fragments of mRNA from Krebs-2 ascites carcinoma cells by an alkylating derivative of nonathymidilyluridine].
It is shown that alkylating reagent 2',3'-O-[4-(N-2-chloroethyl-N-methylamino)]-benzylidene nonathymidilyluridine penetrates into the Krebs-2 ascite carcinoma cells and efficiently alkylates their polymers. Nearly 30% of the reagent penetrated into the cell is consumed by nucleic acids. In conditions providing stability of the complementary complexes the modification extent of poly(A) fragments is two orders of magnitude greater than that of other nucleic acids fractions. No destruction of the oligonucleotide moiety of the reagent occurs in the course of intracellular alkylation.
D000477:Alkylating Agents / Q000494:pharmacology*; D000478:Alkylation; D000818:Animals; D002287:Carcinoma, Krebs 2 / Q000378:metabolism*; D003470:Culture Media; D066298:In Vitro Techniques; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology*; D011061:Poly A / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000378:metabolism*
D000477:Alkylating Agents; D003470:Culture Media; D009588:Nitrogen Mustard Compounds; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011061:Poly A; C042244:2',3'-O-(4-(N-(2-chloroethyl)-N-(methylamino))benzylidene)nonathymidyluridine
Napravlennaia modifikatsiia poliadenilovykh fragmentov informatsionnoĭ RNK v kletkakh astsitnoĭ kartsinomy Krebs-2 alkiliruiushchim proizvodnym nonatimidililuridina.