Metabolism of nitropolycyclic aromatic hydrocarbons by human intestinal microflora.
Anaerobic bacterial suspensions from human and rat feces and intestinal contents, and pure cultures of anaerobic bacteria metabolized 1-nitropyrene and 6-nitrobenzo[a]pyrene to 1-aminopyrene and 6-aminobenzo[a]pyrene, respectively. The metabolites were isolated by reversed-phase high performance liquid chromatography and identified by comparison of their chromatographic and mass spectral properties with those of authentic compounds. The results suggest that anaerobic intestinal bacteria could play a significant role in the metabolism of potentially carcinogenic nitropolycyclic aromatic hydrocarbons.
D000693:Anaerobiosis; D000818:Animals; D001419:Bacteria / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D013058:Mass Spectrometry; D011083:Polycyclic Compounds / Q000378:metabolism*; D051381:Rats; D013045:Species Specificity; D014316:Tritium
D011083:Polycyclic Compounds; D014316:Tritium