Correlation of amikacin concentrations in perilymph and plasma of continuously infused guinea pigs.
A commercially available radioimmunoassay kit was modified to enable us to measure, in triplicate, the amikacin concentration in 1 microliter of perilymph fluid. Amikacin levels in plasma and perilymph were measured in guinea pigs after continuous intravenous infusion at four different dosing rates. After a 4-h infusion, a good linear correlation was found between the amikacin concentration in plasma and the dosing rate. Likewise, a significant linear relationship was found between concentrations of amikacin in perilymph and plasma (y = 0.21x + 2.56; r = 0.67; n = 45) after 6 h of infusion. These results suggest nonsaturation kinetics at the concentrations used.
D000583:Amikacin / Q000097:blood / Q000378:metabolism*; D000818:Animals; D006168:Guinea Pigs; D007263:Infusions, Parenteral; D007612:Kanamycin / Q000031:analogs & derivatives*; D007700:Kinetics; D007761:Labyrinthine Fluids / Q000378:metabolism*; D008297:Male; D010498:Perilymph / Q000032:analysis / Q000378:metabolism*; D011863:Radioimmunoassay
D007612:Kanamycin; D000583:Amikacin