[3H-thymidine paper strip modification of the lymphocyte transformation test. III. Whole blood lymphocyte mixed culture using a cryopreserved stimulating cell mixture].
In comparison to the classical mixed lymphocyte culture (MLC) method a MLC technique using whole blood cultures of responding cells and a pool of prepared stimulating cells from 10 healthy blood donors is described. The reproducibility of cryopreservation of stimulating lymphocytes was proven by means of cell electrophoresis.
D001793:Blood Preservation / Q000379:methods; D005615:Freezing; D006801:Humans; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed / Q000379:methods*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D010835:Phytohemagglutinins; D014316:Tritium; D013936:Thymidine
3H-Thymidin-Papierplättchen-Modifikation des Lymphozytentransformationstests. III. Vollblut-Lymphozytenmischkultur unter Verwendung eines tieftemperaturkonservierten Stimulatorzellgemisches.