Solubilization of immune precipitates by six isolated alternative pathway proteins.
Immune precipitates were solubilized by the alternative pathway of complement assembled from isolated proteins, i.e., C3, factor B, factor D, properdin, C3b inactivator (C3bINA), and beta 1H. The kinetic curves of solubilization in the isolated system and in EGTA-serum were virtually indistinguishable. No requirement of other factors was apparent. Deletion of C3bINA and beta 1H from the complete mixture caused total consumption of C3 in the fluid phase and resulted in neither C3 binding to the complexes nor solubilization. Thus, the presence of a regulated fluid-phase reaction is essential for efficient fixation of C3 and the consequent solubilization. In addition, properdin plays an essential role in the complement-mediated solubilization in the presence of the two regulators. A large amount of C3 was incorporated into the antigen-antibody lattice. Solubilization of immune complexes started after the binding of one C3 molecule to one antibody molecule in the complexes, and the molar ratio of C3:antibody in the solubilized complexes also is approximately 1.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D011232:Chemical Precipitation; D003167:Complement Activation; D003176:Complement C3 / Q000378:metabolism; D003180:Complement C3b Inactivator Proteins / Q000378:metabolism; D011415:Complement Factor B / Q000378:metabolism; D011416:Complement Factor D / Q000378:metabolism; D017242:Complement Factor H; D003170:Complement Pathway, Alternative; D006168:Guinea Pigs; D006801:Humans; D011414:Properdin / Q000378:metabolism; D011817:Rabbits; D012995:Solubility
D000936:Antigen-Antibody Complex; D001798:Blood Proteins; C493495:CFH protein, human; D003176:Complement C3; D003180:Complement C3b Inactivator Proteins; D011414:Properdin; D017242:Complement Factor H; C493488:CFD protein, human; D011416:Complement Factor D; D011415:Complement Factor B