Characterization of a porcine genomic clone encoding a major histocompatibility antigen: expression in mouse L cells.
A porcine genomic clone encoding a major histocompatibility, (MHC) antigen was isolated by direct screening of a swine genomic library with a heterologous human MHC cDNA probe. Mouse L cells transformed with DNA from the clone stably express swine MHC antigen. Pig alloantisera specifically lyse transformant but not control cell lines in a complement-mediated cytotoxicity assay. Direct immunoprecipitation of radiolabeled cellular protein from transformed lines by pig alloantiserum results in the coprecipitation of swine MHC heavy chain and mouse beta 2-microglobulin, demonstrating the association of heterologous subunits of MHC antigens.
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005796:Genes; D006649:Histocompatibility Antigens / Q000235:genetics*; D007739:L Cells; D051379:Mice; D009693:Nucleic Acid Hybridization; D013552:Swine / Q000235:genetics / Q000276:immunology*
D006649:Histocompatibility Antigens