Liquid chromatographic determination of lidocaine in serum.
A method is described for the quantitation of lidocaine in serum by high performance liquid chromatography (HPLC). The analysis utilizes UV detection at 210 nm with a Whatman ODS-3 5-micron analytical column and reverse phase precolumn held at 60 degrees C. The mobile phase consists of acetonitrile, potassium dihydrogen phosphate buffer, phosphoric acid and water. One hundred microlitres of serum sample are required and the analysis time is rapid, at eight minutes. Serum extraction is a two-step process using chloroform, sodium carbonate and hydrochloric acid. This method provides selectivity and sensitivity with a precision of 2.0%, recovery of 97.4%, average accuracy of 98.6% and no interference from eighteen commonly administered drugs.
D001145:Arrhythmias, Cardiac / Q000097:blood / Q000188:drug therapy; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004347:Drug Interactions; D006801:Humans; D008012:Lidocaine / Q000097:blood* / Q000627:therapeutic use; D012015:Reference Standards
D008012:Lidocaine