Ultrastructural cytochemistry of perinucleolar dense spots in heat-treated macroconidia of Neurospora crassa.
Upon heat treatment at 46 degrees C of Neurospora macroconidia, numerous dense sports appear around the nucleolus but disappear completely 5 h after shift-down to normal temperature. Cytochemical reactions and high-resolution autoradiography after pulse-labeling with tritiated uridine indicate that these dense sports contain RNA (perhaps pre-rRNA), RNP and other proteins, but no DNA.
D001345:Autoradiography; D004271:DNA, Fungal / Q000032:analysis; D006651:Histocytochemistry; D006358:Hot Temperature; D009491:Neurospora / Q000648:ultrastructure*; D009492:Neurospora crassa / Q000648:ultrastructure*; D011402:Pronase / Q000378:metabolism; D012331:RNA, Fungal / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D012261:Ribonucleoproteins / Q000032:analysis
D004271:DNA, Fungal; D012331:RNA, Fungal; D012261:Ribonucleoproteins; D012260:Ribonucleases; D011402:Pronase