[Differences in the degrees of stability of colchicine-resistant clones of murine hepatoma XXIIa].
Cells resistant to colchicine in the parental line of mouse hepatoma XXIIa could be revealed with a frequency of 4-4.5 per 10(5) cells when selected at the drug concentration as high as 0.05 mkg/ml. MNNG as a mutagene was shown to increase the number of resistant cells by 5-6 times. 6 clones of independent origin differed in the level of resistance and in the stability to retain it under non-selective conditions. Multistep selection from the stable clones via 0.25, 1.0, 4.0, 5.0, 10.0 and 20.0 mkg/ml resulted in the appearance of some highly resistant subclones. Stable clones of all the steps of selection appeared to be resistant to ethidium bromide. Genetical polymorphism of colchicine-resistance is suggested in the line of mouse hepatoma XXIIa.
D000818:Animals; D002460:Cell Line; D002999:Clone Cells / Q000187:drug effects; D003078:Colchicine / Q000037:antagonists & inhibitors*; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D004996:Ethidium / Q000037:antagonists & inhibitors; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D008769:Methylnitronitrosoguanidine; D051379:Mice; D013997:Time Factors
D008769:Methylnitronitrosoguanidine; D004996:Ethidium; D003078:Colchicine
Razlichiia stepen' stabil'nosti ustoĭchivykh k kolkhitsinu klonov myshinoĭ gepatomy XXIIa.