Purification and characterization of the proteolipid of rabbit sarcoplasmic reticulum.
The proteolipid of rabbit sarcoplasmic reticulum was isolated and characterized. Tyrosine was identified as the C-terminal amino acid by hydrazinolysis and carboxypeptidase A digestion. The N-terminal sequence of proteolipid is: Met-Glx-Arg-Ser-Thr-Arg-Glx-Leu-Cys-Leu-Asp-Phe. The hydrophilic character of the N-terminal portion suggests that it is exposed on the membrane surface.
D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins / Q000032:analysis; D000252:Calcium-Transporting ATPases / Q000032:analysis; D003619:Dansyl Compounds; D010446:Peptide Fragments / Q000032:analysis; D011510:Proteolipids / Q000302:isolation & purification*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000032:analysis* / Q000201:enzymology; D014357:Trypsin
D002135:Calcium-Binding Proteins; D003619:Dansyl Compounds; D010446:Peptide Fragments; D011510:Proteolipids; D014357:Trypsin; D000252:Calcium-Transporting ATPases