Subcellular distribution of the cytochrome P-450 complex and glutathione peroxidase activity in vitamin E and essential fatty acid deficiency.
Glutathione peroxidase (EC 1.11.1.9.) (GSHPx) and P-450 activity were measured in hepatic mitochondrial and microsomal fractions from rats deficient in vitamin E and/or essential fatty acids (EFA). The data were compared to corresponding normal values. GSHPx was significantly decreased in the mitochondrial matrix from animals in all 3 deficiency states. In vitamin E deficiency, a non-significant decreased GSHPx activity was found in mitochondrial membranes. Opposite to these findings, GSHPx was significantly increased in mitochondrial membranes of EFA-deficient animals. In combined EFA and vitamin E deficiency, the mitochondrial membrane GSHPx activity was only insignificantly increased. The P-450 complex activity was not detectable in the mitochondrial matrix. In mitochondrial membranes and microsomes, the P-450 complex activity changed parallel to the GSHPx activity.
D000818:Animals; D000840:Anisoles / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003640:Dealkylation; D005228:Fatty Acids, Essential / Q000172:deficiency*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D007425:Intracellular Membranes / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D010544:Peroxidases / Q000378:metabolism*; D051381:Rats; D013047:Specific Pathogen-Free Organisms; D014811:Vitamin E Deficiency / Q000150:complications / Q000201:enzymology*
D000840:Anisoles; D005228:Fatty Acids, Essential; D003577:Cytochrome P-450 Enzyme System; D010544:Peroxidases; D005979:Glutathione Peroxidase; C005306:4-nitroanisole