Solubility of (1 leads to 3)-beta-D/(1 leads to 6)-beta-D-glucan in fungal walls: importance of presumed linkage between glucan and chitin.
In Saccharomyces cerevisiae, Neurospora crassa, Aspergillus nidulans and Coprinus cinereus most of the alkali-insoluble (1 leads to 3)-beta-D/(1 leads to 6)-beta-D-glucan of the wall can be extracted with dimethyl sulphoxide. The same fraction, and in Saccharomyces cerevisiae a small additional fraction, can be extracted by a destructive procedure involving 40% NaOH at 100 degrees C. The small fraction of the glucan which resists this treatment becomes soluble after a subsequent treatment with HNO2 indicating that it is covalently linked to chitin in the wall. In contrast, in Schizophyllum commune and Agaricus bisporus, nearly all the (1 leads to 3)-beta-D/(1 leads to 6)-beta-D-glucan appears to be held insoluble by linkage to chitin.
D000363:Agaricales / Q000378:metabolism; D001233:Aspergillus nidulans / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D002686:Chitin / Q000378:metabolism*; D005658:Fungi / Q000378:metabolism*; D005936:Glucans / Q000378:metabolism*; D009492:Neurospora crassa / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012995:Solubility
D005936:Glucans; C033671:epiglucan; D002686:Chitin