Human T-cell subset requirements for the production of specific anti-influenza virus antibody in vitro.
Studies were undertaken to define the helper T-cell requirements for in vitro specific antibody production to influenza virus. Subpopulations of human T cells were separated on the basis of their reactivity with the monoclonal antibody OKT4. B cells cultured with OKT4+ T cells produced specific antibody to influenza virus, while B cells cultured with OKT4- T cells did not. Irradiation (1200 rads) of the OKT4- subset to potentially eliminate suppressor-cell activity did not augment the helper-cell function of that subset. Thus, unlike the cytotoxic T-cell response to influenza, help for an in vitro antibody response is mediated only by OKT4+ T cells.
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000918:Antibody Specificity; D002469:Cell Separation; D005434:Flow Cytometry; D006801:Humans; D009980:Influenza A virus / Q000276:immunology*; D008875:Middle Aged; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology* / Q000528:radiation effects; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral