Separation of bovine brain mono- and diacylglycerol lipases by heparin sepharose affinity chromatography.
Mono- and diacylglycerol lipases are differentially inhibited by heparin. No other glycosaminoglycan resembles heparin in this respect. Mono- and diacylglycerol lipases can be separated by heparin Sepharose affinity chromatography. Diacylglycerol lipase was completely retained on a heparin--Sepharose column and was eluted with either 0.5 M NaCl or 2-5 mg/ml heparin, whereas monoacylglycerol lipase was recovered in the washings. Adenosine phosphates markedly affected the activity of diacylglycerol lipase in a concentration dependent manner. ATP was the most potent inhibitor followed by ADP. AMP had no effect and cAMP slightly stimulated the diacylglycerol lipase.
D000227:Adenine Nucleotides / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000302:isolation & purification*; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D006493:Heparin / Q000494:pharmacology; D007700:Kinetics; D008049:Lipase / Q000302:isolation & purification* / Q000378:metabolism; D008861:Microsomes / Q000201:enzymology; D008994:Monoacylglycerol Lipases / Q000302:isolation & purification* / Q000378:metabolism; D012685:Sepharose / Q000031:analogs & derivatives
D000227:Adenine Nucleotides; C037405:heparin-sepharose; D006493:Heparin; D012685:Sepharose; D002265:Carboxylic Ester Hydrolases; D008994:Monoacylglycerol Lipases; D008049:Lipase