Effects of azasteroids on growth and development of the free-living stages of Nippostrongylus brasiliensis and Nematospiroides dubius.
25-Azasteroids were evaluated for their effects on the growth and development of the free-living stages of Nippostrongylus brasiliensis and Nematospiroides dubius. Increasing the concentration of 25-azasteroids in axenic cultures of either species resulted in a decrease in the percentage and mean length of larvae that developed to the third stage. Morphologic abnormalities of inhibited larvae were similar to those shown by larvae cultured in sterol-deficient medium. Addition of cholesterol to the culture medium reversed the inhibitive effects of azasteroid. Azasteroid completely inhibited growth and development of N. brasiliensis when the only sterol present in the culture medium was sitosterol. These results suggest similar pathways of sterol metabolism and similar mechanisms of action by azasteroids in the nematodes and insects that have been studied.
D000818:Animals; D001378:Azasteroids / Q000494:pharmacology*; D002784:Cholesterol / Q000494:pharmacology; D003470:Culture Media; D004357:Drug Synergism; D007814:Larva / Q000187:drug effects; D008297:Male; D009348:Nematoda / Q000254:growth & development*; D009559:Nippostrongylus / Q000254:growth & development*; D051381:Rats; D012855:Sitosterols / Q000494:pharmacology; D013260:Steroids, Heterocyclic / Q000494:pharmacology*
D001378:Azasteroids; D003470:Culture Media; D012855:Sitosterols; D013260:Steroids, Heterocyclic; C025473:gamma-sitosterol; D002784:Cholesterol