Utilization of Barritt color reaction for studying synaptosomal creatine phosphokinase.
The assay for creatine phosphokinase activity depending upon the color formation from creatine was reinvestigated. Its sensitivity was improved to 5 nmol of creatine. The method gave satisfactory Michaelis constants for both creatine phosphate and adenosine-diphosphate and was useful in detecting low creatine phosphokinase activity.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002021:Buffers; D002417:Cattle; D002729:Chloromercuribenzoates / Q000494:pharmacology; D003124:Colorimetry / Q000379:methods; D003402:Creatine Kinase / Q000378:metabolism*; D004229:Dithiothreitol / Q000494:pharmacology; D006168:Guinea Pigs; D007202:Indicators and Reagents; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013574:Synaptosomes / Q000201:enzymology*; D020245:p-Chloromercuribenzoic Acid
D002021:Buffers; D002729:Chloromercuribenzoates; D007202:Indicators and Reagents; D020245:p-Chloromercuribenzoic Acid; D003402:Creatine Kinase; D004229:Dithiothreitol