[Isolation of various fractions of human placental nuclei and their utilization in an in vitro transcription system].
A new approach to isolating chromatin and DNA from human placental nuclei is described. Criteria of purity are given by spectrophotometry and chemical analysis. Chromatin and DNA keep their template efficiencies after isolation. We used them in a transcription system where wheat germ RNA polymerase intervenes. The role of histones was tested on this system.
D002458:Cell Fractionation; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000235:genetics; D005260:Female; D006801:Humans; D007700:Kinetics; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D014158:Transcription, Genetic; D014461:Ultracentrifugation / Q000379:methods
D002843:Chromatin; D004247:DNA
Isolement de différentes fractions nucléaires du placenta humain, et leur utilisation dans un système de transcription in vitro.