Regulatory role of lysosomal enzymes in rat lymphocyte reactivity in vitro.
The influence of three lysosomal enzymes, beta-glucuronidase, acid phosphatase and hialuronidase, the representatives of lysosomal enzymes which can be released by exocytosis, was tested o reactivity of rat lymph node lymphocytes in vitro. These three enzymes do not change the spontaneous lymphocyte stimulation. However, beta-glucuronidase enhances the lymphocyte response to PHA. On the contrary, acid phosphatase and hialuronidase suppress PHA-induced stimulation of lymphocytes. In the mixed lymphocyte cultures (MLC) beta-glucuronidase enhances or suppresses DNA synthesis depending on the degree of lymphocyte stimulation in control MLC. Acid phosphatase and hialuronidase suppress lymphocyte stimulation in MLC.
D000135:Acid Phosphatase / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D002470:Cell Survival; D005966:Glucuronidase / Q000378:metabolism / Q000494:pharmacology; D006821:Hyaluronoglucosaminidase / Q000378:metabolism / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D010835:Phytohemagglutinins; D000135:Acid Phosphatase; D005966:Glucuronidase; D006821:Hyaluronoglucosaminidase