Technique to examine human sperm chromosomes using zona-free hamster eggs.
In experiments with repeated semen samples from two donors, zona-free hamster eggs were used for activation of human spermatozoa to metaphase. When mean number of spermatozoa per penetrated egg was low (1.0-3.3) after insemination in vitro, further incubation resulted in activation of spermatozoa to metaphase. High sperm/egg ratios, 4.2 and 7.5, yielded no sperm chromosomes. The preincubation and insemination intervals necessary for low sperm/egg ratios were individual.
D000818:Animals; D002875:Chromosomes; D006224:Cricetinae; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007621:Karyotyping / Q000379:methods; D008297:Male; D008647:Mesocricetus; D008677:Metaphase; D010063:Ovum / Q000502:physiology*; D013094:Spermatozoa / Q000648:ultrastructure*