beta 1H-hemagglutination assay: a highly sensitive test for human beta 1H and its uses.
Particle-bound human C3b (EAC14oxy23b) serves as the carrier for the detection of biologically active human beta 1 H by IgG-anti-beta 1H. This hemagglutination assay is highly sensitive (greater than or equal to 2 p of beta 1H/40 microliters), easy to perform, rapid, highly reproducible, and needs no expensive laboratory equipment. All reagents are commercially available. The correlation of the beta 1H-hemagglutination assay is applicable for the detection of beta 1H in various buffer systems and may be used for the detection of beta 1H in serum or fractions thereof. It is a very useful tool for the detection of trace contamination of beta 1H in serum fractions, particularly in complement preparations.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D001665:Binding Sites; D001769:Blood; D002417:Cattle; D011232:Chemical Precipitation; D003179:Complement C3b / Q000378:metabolism; D003180:Complement C3b Inactivator Proteins / Q000276:immunology*; D017242:Complement Factor H; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D006801:Humans; D051379:Mice; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; C493495:CFH protein, human; D003180:Complement C3b Inactivator Proteins; D003179:Complement C3b; D017242:Complement Factor H