Synthesis of [4-15NH2]- and [1,3-15N2]cytidine derivatives for use in NMR-monitored binding tests.
The conversion of 15N-enriched urea to a [1,3-15N2]cytidine derivative is accomplished in nine steps with an overall yield of 67%. Synthesis of a [4-15NH2]cytidine derivative is achieved by thiolation of the corresponding uridine derivative with Lawesson's reagent and amination with 15N-enriched ammonia in a sealed glass vessel.
D003562:Cytidine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D007553:Isotope Labeling / Q000379:methods; D009682:Magnetic Resonance Spectroscopy; D009587:Nitrogen Isotopes; D013438:Sulfhydryl Compounds; D014508:Urea
D009587:Nitrogen Isotopes; D013438:Sulfhydryl Compounds; D003562:Cytidine; D014508:Urea