Preparation and electrophysiological characterization of cardiac cell cultures derived from the fetal canine ventricle.
We have developed a primary cardiac cell culture preparation using the fetal canine ventricle. These cultures exhibited widespread and synchronous spontaneous contractile activity, were sensitive to isoproterenol and had the following transmembrane action potential characteristics (means +/- SE): maximum diastolic potential (MDP) -70.9 +/- 0.9 mV; maximum velocity of phase 0 (Vmax) 84 +/- 4 V/s (and tetrodotoxin sensitive); amplitude (AMP) 98.9 +/- 1.1 mV; duration at half amplitude (APD50) 244 +/- 10 ms at a spontaneous cycle length of 1,600 +/- 100 ms. APD50 was linearly dependent on spontaneous cycle length (CL) (slope = 67 ms/s). The action potential of the intact fetal canine ventricle of comparable gestational age had MDP = -67.0 +/- 1.1 mV, Vmax = 127 +/- 6 V/s, AMP = 92.2 +/- 1.6 mV and APD50 = 153 +/- 2 ms at a paced CL of 500 ms. We conclude that myocardial cultures derived from the fetal dog ventricle exhibit electrophysiological characteristics similar to those of the intact canine ventricle, thereby making them a possible alternative to avian and rodent cardiac cultures.
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002395:Catecholamines / Q000494:pharmacology; D002478:Cells, Cultured; D004285:Dogs; D005260:Female; D005333:Fetus; D007545:Isoproterenol / Q000494:pharmacology; D008839:Microelectrodes; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium; D011247:Pregnancy; D013779:Tetrodotoxin / Q000494:pharmacology; D016276:Ventricular Function
D002395:Catecholamines; D013779:Tetrodotoxin; D007545:Isoproterenol