Effects of mercury bichloride on mouse kidney polyribosome structure and function.
Polyribosome sedimentation pattern and their in vitro protein synthetic ability were investigated in kidneys of mice treated with a single injection of HgCl2. Mercury bichloride, after 1 h, evokes polyribosome disaggregation, the extent of which is logarithmically correlated with the dose in the range of 2.5-20 micromoles/kg. With the dose of 2.5 micromoles/kg the effect occurs in 1 h, it is maximal between 1 h and 3 h. After 6 h polyribosomes are reaggregated. Cycloheximide pretreatment does not prevent the HgCl2 induced disaggregation of kidney polyribosomes. The cell-free system derived from kidneys of HgCl2 treated mice (10 micromoles/kg, 1 h) has a decreased protein synthetic ability. Both, in livers of mice treated with 20 micromoles/kg HgCl2 and in isolated rat's reticulocytes incubated with 20 micro M HgCl2 during 1 h there were no apparent changes in the polyribosome sedimentation patterns.
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D007668:Kidney / Q000648:ultrastructure; D008099:Liver / Q000648:ultrastructure; D008297:Male; D008627:Mercuric Chloride; D008628:Mercury / Q000633:toxicity*; D051379:Mice; D011132:Polyribosomes / Q000187:drug effects* / Q000648:ultrastructure; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism; D012156:Reticulocytes / Q000648:ultrastructure
D011506:Proteins; D008627:Mercuric Chloride; D003513:Cycloheximide; D008628:Mercury