Monoclonal antibody localization in subcutaneous and intracranial human glioma xenografts: paired-label and imaging analysis.
The murine antiglioma monoclonal antibody 81C6 has been shown to specifically localize in U-251 MG and D-54 MG human glioma subcutaneous and intracranial athymic mouse xenografts expressing the human gliomamesenchymal extracellular matrix glycoprotein GMEM. In paired-label studies 81C6 reached peak levels of localization in subcutaneous and intracranial xenografts in 24 to 48 hours and persisted there for an additional 5 to 7 days before declining. The percent localized 81C6 in U-251 MG subcutaneous xenografts was tumor size dependent ranging from 2% in 200-300 mg tumors to 15% in 1 gram tumors. Subcutaneous xenografts of U-251 MG were readily radioimaged from 1 up to 6 days following administration of 131-I-81C6. The specificity of 81C6 localization has also been demonstrated by tissue autoradiography, elution of radiolabeled 81C6 from tumor xenografts, and in vivo inhibition of radiolabeled 81C6 localization by unlabeled 81C6. D-54 MG intracranial xenografts were permeable to 81C6 and control immunoglobulin but specific localization occurred only with 81C6. Radiolabeled 81C6 monoclonal antibody and U-251 MG and D-54 MG human glioma xenografts provide a useful operationally tumor specific monoclonal antibody tumor localization model for the examination of monoclonal antibody pharmacokinetics, radioimaging, radioimmunotherapy and combined modality therapy in intracranial human glioma xenografts.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D001345:Autoradiography; D001932:Brain Neoplasms / Q000276:immunology*; D002460:Cell Line; D005910:Glioma / Q000000981:diagnostic imaging / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007457:Iodine Radioisotopes; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011877:Radionuclide Imaging; D012878:Skin Neoplasms / Q000000981:diagnostic imaging / Q000276:immunology*; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes