[Molecular mechanisms of the radiation destruction and formation of cross links in chromatin in aqueous solutions].
The modified gel-electrophoresis techniques were used to study DNA destruction in oligonucleosomes of the chromatin and the formation of DNA-protein cross-links under the effect of 60Co-gamma-rays. The yields of DNA destruction were evaluated in different conditions of chromatin irradiation: they were comparable with the yields of single-strand breaks. The bonds in the DNA-protein polymer formed were found to be covalent. It was shown that the processes of formation of cross-links and peroxide radicals (hydroperoxides) were mutually exclusive.
D000818:Animals; D002843:Chromatin / Q000528:radiation effects*; D004247:DNA / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D066298:In Vitro Techniques; D008099:Liver / Q000528:radiation effects; D051379:Mice; D008968:Molecular Conformation / Q000528:radiation effects; D011506:Proteins / Q000528:radiation effects; D012996:Solutions
D002843:Chromatin; D011506:Proteins; D012996:Solutions; D004247:DNA
O molekuliarnykh mekhanizmakh radiatsionnoĭ destruktsii i obrazovaniia sshivok v khromatine v vodnykh rastvorakh.