Hepatocytes (hepatic parenchymal cells) produce a major part of liver collagen in vivo.
Following intraperitoneal injection of [3H]proline and colchicine, rat liver cells were dispersed by collagenase perfusion and fractionated by low-speed and density gradient centrifugation. Analysis of the collagenous components using purified bacterial collagenase showed that 0.1 to 0.2% of the labeled protein produced by the liver cells was collagen. Considering the population of the hepatocytes in the liver (70%), 80% of the collagen produced by the liver could be attributed to the hepatocytes.
D000818:Animals; D003078:Colchicine / Q000494:pharmacology; D003094:Collagen / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011392:Proline / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D014316:Tritium; D003094:Collagen; D011392:Proline; D003078:Colchicine