Purification and multiple forms of human placental alkaline phosphatase.
The commercially available human placental alkaline phosphatase was purified to near homogeneity. Multiple bands of the purified enzyme were resolved in the polyacrylamide gel. The number of bands in the gel was reduced after the enzyme was treated with neuraminidase.
D000469:Alkaline Phosphatase / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D004589:Electrophoresis, Disc; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification*; D008970:Molecular Weight; D009439:Neuraminidase; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy
D007527:Isoenzymes; D000469:Alkaline Phosphatase; D009439:Neuraminidase