Genetic and serologic re-evaluation of LyM-1 antigen specified by a gene closely linked to Mls: establishment of LyM-1 antigen of the mouse.
An alloantiserum prepared against LyM-1 antigen of the mouse has been analysed in detail. This antiserum, C3H/HeJ anti-CBA/J, has turned out to define two antigens which are specified by independently segregating loci. One of these is Lyb-2.1 or similar antigen specified by a gene located on chromosome 4. The other is identified as LyM-1.2 based on the result that a gene determining alloantigenicity in the Lyb-2- strain is closely linked to Mls with recombination frequency of 0.066 +/- 0.028. Since both antigens are expressed preferentially on B lymphocytes and their strain distribution is significantly overlapped, there has been much confusion about LyM-1 antigen. This is overcome by re-evaluating anti-LyM-1.2 serum under the condition in which Lyb-2.1 is not involved in analysis. The strain distribution of LyM-1 antigen, its preferential expression on B lymphocytes and close genetic linkage of LyM-1 to Mls have been established.
D000818:Animals; D000918:Antibody Specificity; D002469:Cell Separation / Q000379:methods; D002874:Chromosome Mapping; D003601:Cytotoxicity Tests, Immunologic; D004592:Electrophoresis, Starch Gel; D005260:Female; D005796:Genes; D007518:Isoantibodies / Q000276:immunology*; D007519:Isoantigens / Q000235:genetics*; D007521:Isocitrate Dehydrogenase / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics; D010447:Peptide Hydrolases / Q000032:analysis
D007518:Isoantibodies; D007519:Isoantigens; D007527:Isoenzymes; D007521:Isocitrate Dehydrogenase; D010447:Peptide Hydrolases