Structure-activity relationship of pyrimidine base analogs as ligands of orotate phosphoribosyltransferase.
Eighty pyrimidine base analogs were evaluated as inhibitors of mouse liver orotate phosphoribosyltransferase (OPRTase, EC 2.4.2.10). Based on these findings and an extensive literature review, a structure-activity relationship has been formulated for the binding of pyrimidine base analogs to OPRTase. This study provides a basis for the rational design of new inhibitors of this enzyme, and several such compounds are proposed. Additionally, 4,6-dihydroxypyrimidine has been found to be a potent OPRTase inhibitor. Eleven OPRTase inhibitors were also evaluated as inhibitors of orotidine 5'-monophosphate decarboxylase (ODCase, EC 4.1.2.23). 5-Azauracil, 5-azaorotate, and barbituric acid inhibited ODCase significantly only after preincubation with PRPP and MgCl2 in the presence of cytosol.
D000818:Animals; D066298:In Vitro Techniques; D007700:Kinetics; D008024:Ligands; D051379:Mice; D008815:Mice, Inbred Strains; D009962:Orotate Phosphoribosyltransferase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000037:antagonists & inhibitors; D010430:Pentosyltransferases / Q000037:antagonists & inhibitors*; D011485:Protein Binding; D011743:Pyrimidines / Q000378:metabolism* / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D014498:Uracil / Q000031:analogs & derivatives / Q000378:metabolism
D008024:Ligands; D011743:Pyrimidines; D014498:Uracil; D010430:Pentosyltransferases; D009962:Orotate Phosphoribosyltransferase; D009964:Orotidine-5'-Phosphate Decarboxylase