[Determination of plasma noradrenaline by automatic high pressure liquid chromatography with electrochemical detection].
A method for the plasma noradrenaline (NA) analysis by reversed-phase liquid chromatography with electrochemical detection is described. The plasma sample preparation includes two-steps: ion-exchange and alumina adsorption. The use of a data system and an automatic sampler enables the injection of samples in series. Using this technique, determination of plasma NA concentration in 34 healthy subjects (27.4 +/- 7.2 years) gave the following results: 0.28 +/- 0.10 micrograms/l after 30 min of resting in the supine position and 0.52 +/- 0.17 micrograms/l after 5 min of upright position.
D000328:Adult; D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D006801:Humans; D009638:Norepinephrine / Q000097:blood*
D009638:Norepinephrine
Détermination de la noradrénaline plasmatique par chromatographie liquide haute pression en mode automatique avec détection électrochimique.