Heterogeneity of stimulator cells in the murine mixed leukocyte response.
Mouse spleen cells were fractionated and tested for their ability to stimulate a primary mixed leukocyte response (MLR). Several nonlymphoid populations were stimulatory, purified dendritic cells and 18-h adherent macrophages being the most effective. B and T lymphocytes and peritoneal washout cells were almost ineffective by comparison. This hierarchy of stimulator potential was observed whether the genetic difference between stimulator and responder was whole H-2 or H-2 subregion (K, D, I, I-J). Allostimulatory populations contained a high proportion of cells expressing I region determinants. The functional importance of these antigens was confirmed using specific anti-I region antisera. Thus, it appears that stimulator cells in the MLR are heterogeneous, Ia-bearing, and of "accessory" rather than lymphoid type.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002448:Cell Adhesion; D002469:Cell Separation; D006183:H-2 Antigens / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology; D007375:Interleukin-1; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000145:classification* / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D011506:Proteins / Q000494:pharmacology; D011961:Receptors, Fc / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology
D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; D007106:Immune Sera; D007375:Interleukin-1; D011506:Proteins; D011961:Receptors, Fc