Continuous perfusion of mammalian cells embedded in agarose gel threads.
A method for perfusing cells by embedding them in fine agarose gel threads is described and characterized. The rate of diffusion of a metabolite into the gel threads is determined by 31P-NMR spectroscopy. This perfusion method is shown to enable Chinese hamster lung fibroblasts (CHLF) to remain in a metabolically active state with high levels of intracellular ATP for many hours.
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D004058:Diffusion; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005782:Gels; D008168:Lung; D009682:Magnetic Resonance Spectroscopy; D008722:Methods; D010477:Perfusion; D012685:Sepharose
D003470:Culture Media; D005782:Gels; D012685:Sepharose