Involvement of some amino acid residues in the enzymatic activity of solubilized cerebral fucosyltransferase.
We have checked the effect of some chemical reagents specific for amino acid residues on the activity of a solubilized cerebral glycoprotein:fucosyltransferase. Diethylpyrocarbonate, 2,3-butanedione and tetranitromethane specific for histidyl, arginyl, and tyrosyl residues respectively, were strong inhibitors of the enzymatic activity This led us to conclude that these amino acid residues are "essential residues" in the cerebral fucosyltransferase activity.
D000818:Animals; D001120:Arginine; D001665:Binding Sites; D001921:Brain / Q000201:enzymology*; D002074:Butanones / Q000494:pharmacology*; D003931:Diacetyl / Q000494:pharmacology*; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology*; D005561:Formates / Q000494:pharmacology*; D005647:Fucosyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D006602:Hexosyltransferases / Q000378:metabolism*; D006639:Histidine; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000494:pharmacology; D007700:Kinetics; D008697:Methane / Q000031:analogs & derivatives*; D012756:Sheep; D013774:Tetranitromethane / Q000494:pharmacology*; D014443:Tyrosine
D002074:Butanones; D005561:Formates; D006898:Hydroxylamines; D019811:Hydroxylamine; D014443:Tyrosine; D006639:Histidine; D001120:Arginine; D005647:Fucosyltransferases; D006602:Hexosyltransferases; C020559:Glycoprotein 6-alpha-L-fucosyltransferase; D013774:Tetranitromethane; D003931:Diacetyl; D004047:Diethyl Pyrocarbonate; D008697:Methane