Partial proteolysis of human L-type phosphofructokinase.
L (liver) type phosphofructokinase subunits purified from human leukocytes are slightly lighter than L subunits from liver and red blood cells. A mild treatment of red blood cell L4 enzyme with subtilisin converts its subunits into forms of similar molecular weight to leukocyte enzyme. From a kinetical point of view, subtilisin-treated L4 phosphofructokinase and leukocyte enzymes are characterized by a decrease of the allosteric properties as compared to non-treated red cell L4 phosphofructokinase.
D000328:Adult; D002384:Catalysis; D004789:Enzyme Activation; D006801:Humans; D007700:Kinetics; D007962:Leukocytes / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008970:Molecular Weight; D010732:Phosphofructokinase-1 / Q000037:antagonists & inhibitors / Q000378:metabolism*
D010732:Phosphofructokinase-1