Inhibition of ADP-induced aggregation of bovine platelets by saturated fatty acids and its relation with the change of membrane surface charge.
The inhibitory effects of saturated fatty acids with 4 to 18 carbon atoms on ADP-induced aggregation of bovine platelets were investigated. The inhibitory effects of the acids increased with increase of their alkyl chain length up to C14. On the other hand, from C16 the inhibitory effects tended to decrease with increase of chain length, and stearic acid (C18) was not inhibitory. There was a linear relationship between the inhibitory effects and alkyl chain lengths up to C12. This linear relation and the slope of the linear regression line suggested that the inhibitory effects of the acids depended on their partition into the membrane. The fatty acids decreased the fluorescence of the surface charge probe 2-p-toluidinylnaphthalene-6-sulfonate, indicating that they increased the negative charge on the membrane surface. The relative effects of the acids on the fluorescence were consistent with their relative inhibitory effects on aggregation. These results suggest that the inhibition of platelet aggregation by saturated fatty acids is due to a change in the membrane surface charge of the platelet plasma membrane.
D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D005227:Fatty Acids / Q000494:pharmacology*; D005454:Fluorescence Polarization; D008433:Mathematics; D008560:Membrane Fluidity / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects*
D005227:Fatty Acids; D000244:Adenosine Diphosphate