In vivo effect of methylcobalamin on the peripheral nerve structure in streptozotocin diabetic rats.
To study in vivo  effect of methylcobalamin (CH3-B12) on the  peripheral nerve structures, rats with experimental diabetes induced by streptozotocin were administered with daily intramuscular injection of CH3-B12 (500 microgram/kg) for 16 weeks. By isolated nerve fiber studies, CH3-B12-treated diabetic rats showed less incidence of paranodal demyelination as an early sign of segmental demyelination than non-treated diabetic rats. From morphometrical analysis on sural nerves, the reduction in the density of myelinated  nerve fibers, nerve fiber size and axon size of myelinated fibers was definitely protected in treated diabetic rats. The results suggested that continuous treatment with CH3-B12 had an ameliorative effect on the peripheral nerve lesions in experimental diabetic neuropathy.
D000818:Animals; D001369:Axons / Q000648:ultrastructure; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D003929:Diabetic Neuropathies / Q000188:drug therapy*; D008297:Male; D009413:Nerve Fibers, Myelinated / Q000648:ultrastructure; D010525:Peripheral Nerves / Q000187:drug effects* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013127:Spinal Nerves / Q000166:cytology; D013311:Streptozocin; D014805:Vitamin B 12 / Q000031:analogs & derivatives* / Q000494:pharmacology
D013311:Streptozocin; C019476:mecobalamin; D014805:Vitamin B 12