Burn toxin and its competitin.
A "toxic factor' has been isolated and purified from scalded normal human skin; it is lethal to mice and toxic to HeLa and HEP2 cell lines in tissue culture. The toxic factor in both crude and purified form is antigenic in rabbits, producing an antisera that neutralizes the in vivo and in vitro effects of "burn toxin', similar to that of the convalescent sera of burned human subjects. The sonicate of the toxic glycoprotein named competitin is relatively non-lethal and protects against the lethal effect of the toxic factor. The action of the purified burn toxic factor and its competitin is at the ATP site of actomyosin preparations. The data presented suggests that the purified burn toxic factor and its competitin compete for the same receptor sites in the myocardium. A thesis is presented that states that it is vital to neutralize the toxic effects of burn breakdown tissue products in severely burned subjects before the vicious cycle of depressed immunological function and malnutrition ensues. Competitin produced in vitro from toxic factor(s) generated scalded normal human skin is offered as a means of neutralizing the toxic factors(s).
D000205:Actomyosin / Q000037:antagonists & inhibitors / Q000276:immunology; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000276:immunology; D000818:Animals; D000992:Antitoxins; D001665:Binding Sites; D002056:Burns / Q000201:enzymology / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008954:Models, Biological; D009206:Myocardium / Q000201:enzymology / Q000276:immunology; D011817:Rabbits; D014118:Toxins, Biological / Q000276:immunology*
D000992:Antitoxins; D006023:Glycoproteins; D014118:Toxins, Biological; D000205:Actomyosin; D000251:Adenosine Triphosphatases