Ontogenesis of muscarinic acetylcholine binding sites in cat visual cortex: reversal of specific laminar distribution during the critical period.
Acetylcholine appears to act as a modulator of neuronal activity in cat visual cortex and, like noradrenaline, may be involved with cortical plasticity mechanisms during the critical period. To explore possible ACh involvement in these mechanisms we have examined acetylcholine binding sites in cat visual cortex during development using [3H]QNB, a muscarinic antagonist. At 3 days postnatal [3H]QNB preferentially labelled binding sites in layer IV. During development the pattern of binding reversed, so that by 95 days postnatal layer IV was the least densely labelled. The number of binding sites increased during development peaking at 1 month postnatal. The Kd of [3H]QNB binding sites increased to 95 days postnatal, with a peak value of 0.76 nM. The results show that during development, and especially within the critical period, changes in [3H]QNB binding site distribution, number and affinity occur.
D000818:Animals; D001345:Autoradiography; D002415:Cats; D007700:Kinetics; D008297:Male; D009473:Neuronal Plasticity; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000378:metabolism / Q000502:physiology*; D014793:Visual Cortex / Q000254:growth & development*
D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate