[Reactive SH-groups in clinical practice. Methodological notes].
Experimental investigations have been performed in order to point out the extrinsic factors influencing the results of -SH group determination. It has been observed that the anticoagulants normally used to obtain plasma samples, influence the serum -SH groups; in particular, heparin increases and citrate depresses the levels; the most reproducible data were obtained in serum samples. In addition it has been shown that the exposure to light for 6-8 hours lowered the serum -SH groups, whereas darkness prevented these changes. Serum-SH groups were not influenced by the administration of non steroidal anti-inflammatory agents, whereas D-Penicillamine and Tiopronin induced an increase of the -SH levels, ranging between 15-20%. The levels returned to the basal values within two hours from the drug intake. Following the methodological criteria here described it is possible to obtain a good information about the -SH group reserve, which free radicals generated in several diseases could consume. The practical usefulness of the serum -SH group determination in oxidative-peroxidative processes in discussed.
D000328:Adult; D000368:Aged; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001172:Arthritis, Rheumatoid / Q000097:blood*; D002951:Citrates / Q000494:pharmacology; D003624:Darkness; D003920:Diabetes Mellitus / Q000097:blood*; D005260:Female; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008027:Light; D008103:Liver Cirrhosis / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010396:Penicillamine / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000032:analysis / Q000097:blood*
D000893:Anti-Inflammatory Agents; D002951:Citrates; D013438:Sulfhydryl Compounds; D006493:Heparin; D010396:Penicillamine
I gruppi-SH reattivi nella pratica clinica. Puntualizzazioni metodologiche.