[Method for selecting cells during various phases of the mitotic cycle].
Exponentially growing L5178Y cells in suspension culture were separated according to their position in the cell cycle on the basis of their volume with a velocity sedimentation method in which a linear and continuous ficoll gradient was used. Highly purified populations of G1 and S cells were obtained, containing about 90% G1 phase cells and 80% S phase cells. The method is rapid and a larger number of cells can be easily processed with no loss of viability.
D000818:Animals; D002452:Cell Count; D002469:Cell Separation; D002470:Cell Survival; D002478:Cells, Cultured; D002498:Centrifugation; D007940:Leukemia L5178; D051379:Mice; D008938:Mitosis
Método de selección de células en distintas fases del ciclo mitótico.