Acid hydrolases in brain, spinal cord and sciatic nerves of acrylamide-intoxicated rats.
Activity of beta-glucuronidase and cathepsin-D were studied in brain, spinal cord and sciatic nerves of rats at 24 h or 10 days after daily i.p. administration of 50 mg/kg acrylamide. The activities of beta-glucuronidase and cathepsin-D in brain, spinal cord and sciatic nerves remained unaffected on single exposure but increased significantly in these tissues on administration of the neurotoxin for 9 consecutive days. The increase in the activity of beta-glucuronidase and cathepsin-D was more marked in sciatic nerves, than brain or spinal cord. These results suggest that cathepsin-D and beta-glucuronidase may be involved in the acrylamide-induced degeneration of nervous tissues and can serve as useful markers for the detection of chemical-induced neuropathies.
D020106:Acrylamide; D000178:Acrylamides / Q000633:toxicity*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002402:Cathepsin D; D002403:Cathepsins / Q000378:metabolism*; D005966:Glucuronidase / Q000378:metabolism*; D008297:Male; D051381:Rats; D012584:Sciatic Nerve / Q000187:drug effects / Q000201:enzymology*; D013116:Spinal Cord / Q000187:drug effects / Q000201:enzymology*; D013997:Time Factors; D014018:Tissue Distribution
D000178:Acrylamides; D020106:Acrylamide; D005966:Glucuronidase; D002403:Cathepsins; D002402:Cathepsin D