GAGs-potentiated inhibition of thrombin, factor Xa and plasmin in plasma and in a purified system containing antithrombin III - correlation with total charge density.
The ability of heparin and related glycosaminoglycans (GAGs) to accelerate the inhibition of thrombin, factor Xa and plasmin in plasma and in a purified system containing antithrombin III (At III) was studied using chromogenic peptide substrate assays. There was good correlation between the charge density of the mucopolysaccharides and the activities investigated. While the difference between potentiation of the antithrombin activity by GAGs in plasma and in the purified system was slight, the inhibition of factor Xa in plasma was more pronounced than in the presence of purified At III, indicating the mechanisms for GAGs-potentiated inhibition of thrombin and factor Xa are not identical. For the antiplasmin activity, there was a good correlation between the chemical structure and biological activity only in the pure system, confirming that the antithrombin-GAG complex plays a very limited role in the inactivation of plasmin in plasma.
D000991:Antithrombins; D002863:Chromogenic Compounds; D005170:Factor X / Q000037:antagonists & inhibitors*; D005341:Fibrinolysin / Q000037:antagonists & inhibitors*; D006025:Glycosaminoglycans / Q000494:pharmacology*; D006801:Humans; D013329:Structure-Activity Relationship; D013917:Thrombin / Q000037:antagonists & inhibitors*
D000991:Antithrombins; D002863:Chromogenic Compounds; D006025:Glycosaminoglycans; D005170:Factor X; D013917:Thrombin; D005341:Fibrinolysin