7-Amino-actinomycin D as a specific fluorophore for DNA content analysis by laser flow cytometry.
A technique for DNA amount determination by flow cytometry based on the use of 7-amino-actinomycin D (7-amino-AMD), a fluorescent analogue of antibiotic actinomycin has been investigated, and a particular staining procedure has been developed. The procedure includes short fixation in 70% ethanol and staining for 20 min in 10(-5)M solution of 7-amino-AMD at pH7. The results of DNA content measurements are very reproducible. The histograms obtained have a coefficient of variation less than 3%. The absorption maximum of the complex of 7-amino-AMD with DNA is situated in the green spectrum region, making this stain particularly suitable for argon laser flow cytometry.
D000818:Animals; D002875:Chromosomes / Q000648:ultrastructure; D004247:DNA / Q000032:analysis* / Q000097:blood; D003609:Dactinomycin / Q000031:analogs & derivatives*; D004330:Drosophila; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes; D007834:Lasers; D008099:Liver / Q000166:cytology; D008214:Lymphocytes / Q000166:cytology; D051379:Mice; D012469:Salivary Glands / Q000166:cytology
D005456:Fluorescent Dyes; D003609:Dactinomycin; C025942:7-aminoactinomycin D; D004247:DNA