[Kinetic differences in the oxidative phosphorylation system of control and ischemia-damaged heart mitochondria].
Rabbit heart mitochondria isolated with or without rotenone (M + R and M -R, respectively) were studied. It was shown with the use of pHmetry that the Vmax of oxidative phosphorylation decreased during ischemia (autolysis for 0.5 h at 37 degrees C) in both preparations of the mitochondria to the same extent. The Km for ADP was unchanged in M - R, but rose significantly in M + R. Carnitine reduced the ischemia -increased Km value. The data suggest the inhibition of adenine nucleotide translocase by longchain acyl-Co A, whose level was found to be elevated by 52% in M + R during ischemia.
D000818:Animals; D003327:Coronary Disease / Q000378:metabolism*; D066298:In Vitro Techniques; D008929:Mitochondria, Heart / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D011817:Rabbits
O kineticheskikh razlichiiakh sistemy okislitel'nogo fosforilirovaniia kontrol'nykh i povrezhdennykh ishemieĭ mitokhondriĭ serdtsa.