tif-Stimulated deoxyribonucleic acid repair in Escherichia coli K-12.
Bacterial survival is significantly increased after ultraviolet irradiation in tif sfi cells, provided that the thermosensitive tif mutation has been expressed at 41 degrees C before irradiation. This tif-mediated "reactivation of ultraviolet irradiated bacteria" needs de novo protein synthesis, as is the case for the tif-mediated reactivation of ultraviolet-irradiated phage lambda. However, in striking contrast to the phage reactivation process, this tif-mediated reactivation is no longer associated with mutagenesis. It also requires the presence of the uvrA+ excision function. These results strongly suggest the existence in Escherichia coli K-12 of a repair pathway acting on bacterial deoxyribonucleic acid which is inducible, error free, and uvr dependent.
D001426:Bacterial Proteins / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics; D004260:DNA Repair; D004269:DNA, Bacterial / Q000528:radiation effects; D004279:DNA, Viral / Q000528:radiation effects; D004926:Escherichia coli / Q000235:genetics*; D006358:Hot Temperature; D009154:Mutation; D011938:Rec A Recombinases; D011995:Recombination, Genetic; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004279:DNA, Viral; D011938:Rec A Recombinases