Isolation and characterization of cholecystokinin-58 (CCK-58) from porcine brain.
A 58-residue peptide has been isolated from extracts of porcine brain and shown to be an N-terminally extended cholecystokinin (CCK). The amino acid sequence of this peptide is: Ala-Val-Gln-Lys-Val-Asp-Gly-Glu-Ser-Arg-Ala-His-Leu-Gly-Ala-Leu-Leu-Ala- Arg-Tyr- Ile-Gln-Gln-Ala-Arg-Lys-Ala-Pro-Ser-Gly-Arg-Val-Ser-Met-Ile-Lys-Asn-Leu- Gln-Ser- Leu-Asp-Pro-Ser-His-Arg-Ile-Ser-Asp-Arg-Asp-Tyr(SO3)-Met-Gly-Trp-Met-Asp -Phe-NH2. The peptide was found to induce contraction of the guinea-pig gallbladder, but the pattern of this action seemed to differ from those of CCK-8 and CCK-33.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D011232:Chemical Precipitation; D002766:Cholecystokinin / Q000302:isolation & purification* / Q000494:pharmacology; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D005704:Gallbladder / Q000502:physiology; D006168:Guinea Pigs; D009119:Muscle Contraction / Q000187:drug effects; D010446:Peptide Fragments / Q000032:analysis; D013552:Swine
D000596:Amino Acids; D010446:Peptide Fragments; C038823:cholecystokinin 58; D002766:Cholecystokinin