A comparative study of protein synthesis in vitro by skeletal muscle ribosomes from livestock.
Skeletal muscle was dissected from the forelimbs of animals and stored at -80 degrees C. Protein synthesis activity was determined in cell-free systems. Incorporation of amino acids into protein was expressed per milligramme of ribosomal RNA, per gramme wet weight of skeletal muscle and per milligramme of DNA. Species related differences in activity were observed in skeletal muscle obtained from pig, horse, lamb, sheep, calf and young steer. Intraspecies differences were seen in cattle of different breeds. The protein composition of the muscle tissue showed quantitative differences between the species. Using biopsy specimens, the system could be used to monitor the protein nutrition status of animals and the production of animal proteins.
D000818:Animals; D002417:Cattle; D002474:Cell-Free System; D004247:DNA / Q000032:analysis; D006736:Horses; D066298:In Vitro Techniques; D008970:Molecular Weight; D009124:Muscle Proteins / Q000096:biosynthesis*; D009132:Muscles / Q000378:metabolism* / Q000648:ultrastructure; D011132:Polyribosomes / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D012756:Sheep; D013552:Swine
D009124:Muscle Proteins; D012335:RNA, Ribosomal; D004247:DNA