Cobalt-ion chelate affinity chromatography for the purification of brain neutral alpha-D-mannosidase and its separation from acid alpha-D-mannosidase.
A method is described for the purification of neutral alpha-D-mannosidase and its separation from acid alpha-D-mannosidase from monkey brain by utilizing Co2+ chelate affinity chromatography. The neutral enzyme, which selectively bound to the metal-ion chelate column, was elutable by Tris at pH 7.5 and gave over 80-fold purification in a single step with 100% recovery.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002846:Chromatography, Affinity / Q000379:methods; D003035:Cobalt; D006863:Hydrogen-Ion Concentration; D008255:Macaca radiata; D008361:Mannosidases / Q000302:isolation & purification*; D043323:alpha-Mannosidase
D003035:Cobalt; D008361:Mannosidases; D043323:alpha-Mannosidase