The influence of melittin on the rotation of band 3 protein in the human erythrocyte membrane.
The rotational mobility of band 3, a protein constituent of the human erythrocyte membrane, was measured by observing the flash-induced transient dichroism of the triplet probe eosin maleimide. In the presence of melittin, a pharmacologically active polypeptide from honey bee (Apis mellifera) venom, a dose-dependent loss of rotational mobility was detected. With acetylated melittin, the ability to immobilize is reduced. Succinylated melittin, however, is devoid of immobilizing activity. The possible relevance of these findings to the normal mode of action of melittin was examined by comparing the relative abilities of the native, acetylated and succinylated melittins to lyse erythrocytes and synergize with phospholipase A2, another constituent of bee venom. For both these properties, the order of effectiveness is native melittin greater than acetyl melittin greater than succinyl melittin = 0, the same as their order of effectiveness in immobilizing band 3. A mechanism is proposed in which melittin is anchored in the membrane by its hydrophobic N-terminus, while its cationic C-terminal moiety binds to negatively charged residues on membrane proteins. This leads either directly or indirectly to protein aggregation and hence loss of mobility. From a detailed comparison of the different effects of the melittin derivatives, it is concluded that melittin may function in vivo by aggregating membrane proteins in order to allow phospholipase A2 to gain access to the membrane bilayer and commence catalysis.
D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D001514:Bee Venoms / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000648:ultrastructure*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008555:Melitten / Q000302:isolation & purification / Q000494:pharmacology*; D010741:Phospholipases A / Q000302:isolation & purification; D054467:Phospholipases A2
D001457:Anion Exchange Protein 1, Erythrocyte; D001514:Bee Venoms; D008555:Melitten; D010741:Phospholipases A; D054467:Phospholipases A2