A study of maturation events in jackbeans (Canavalia ensiformis).
Maturation events have been studied in developing jackbean (Canavalia ensiformis) cotyledons by using a combination of analysis by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, overlays with 125I-concanavalin A (Con A) and the use of anti-(Con A) after Western transfer. The number of polypeptides recognized by 125I-Con A varies during maturation, until at maturity only one remains. Several molecular forms of the lectin occur during development; one, corresponding to Mr 33 000 and found only in immature seeds, interacts with 125I-Con A, suggesting that it is glycosylated.
D003208:Concanavalin A / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae; D010455:Peptides / Q000378:metabolism; D037121:Plant Lectins; D010946:Plants, Medicinal; D012639:Seeds / Q000254:growth & development / Q000378:metabolism*
D010455:Peptides; D037121:Plant Lectins; D003208:Concanavalin A