Subcellular distribution of neuroactive amino acids in brains of genetically epileptic rats.
The subcellular distribution of amino acids was compared in brains of genetically seizure-susceptible (SS) and genetically seizure-resistant (SR) rats. The total taurine content (mumol/brain) in the P2B, or synaptosomal, fraction in SS rats was only 37% of that of SR rats. Glutamate, glutamine, glycine, alanine, and gamma-aminobutyric acid (GABA) contents were unaltered. No alterations in total content were found in other subcellular fractions for the amino acids studied. SS animals that had never been stimulated to audiogenic seizure had decreased concentrations of taurine (nmol/mg protein) in the P2, P2B, and P2C fractions as compared with SR animals. These fractions contain crude synaptosomes, enriched synaptosomes, and enriched mitochondria, respectively. Phosphoethanolamine concentrations were also decreased in the P2B fractions, but concentrations of other amino acids were unaltered, as compared with SR animals. Twenty-four hours after the intracerebroventricular injection of taurine (6 mumol) in SS animals that had never been convulsed, taurine concentrations were significantly increased in whole brain homogenate and P2 and P2B fractions as compared with SS animals not given taurine. This treatment left unaltered the concentrations of glutamate, glutamine, GABA, and glycine in brain homogenate and P2 fraction. Because decreases in taurine concentration were seen in animals that had not been convulsed, these alterations are intrinsic to the SS strain and are not a consequence of convulsive activity. In view of the antiepileptic action of taurine, and the fact that an impairment of taurine transport in the brain of SS rats had previously been demonstrated, we suggest that a defect in the biochemistry of taurine is partially responsible for the seizure susceptibility of the SS rat.
D000409:Alanine / Q000032:analysis; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D004827:Epilepsy / Q000235:genetics / Q000378:metabolism*; D005971:Glutamates / Q000032:analysis; D005973:Glutamine / Q000032:analysis; D005998:Glycine / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013654:Taurine / Q000032:analysis; D005680:gamma-Aminobutyric Acid / Q000032:analysis
D000596:Amino Acids; D005971:Glutamates; D005973:Glutamine; D013654:Taurine; D005680:gamma-Aminobutyric Acid; D000409:Alanine; D005998:Glycine