[Standardization of indirect immunofluorescence for detection of autoantibodies (author's transl)].
Seven laboratories made a chessboard titration with a serum containing antimitochondrial antibodies and two conjugates labeled with fluorescein isothiocyanate. The variation of the plateau titer and the titer of the serum as well as the plateau-end point was slight with the exception of one case. The standardization of indirect immunofluorescence needs a close contact between the laboratories, the availability of reference sera and conjugates, and similar microscopic equipment.
D001323:Autoantibodies / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans
D001323:Autoantibodies
Ringversuch zum Nachweis von Autoantikörpern durch indirekte Immunfluoreszenz.