Glucose and sucrose effects in the culture media on Streptococcus sanguis OMZ 9 glucosyltransferase inhibition by anti-glucosyltransferase antibodies.
An anti-glucosyltransferase serum was prepared against a purified enzyme of Streptococcus sanguis OMZ 9. Antibody fractions obtained after gel filtration on a Bio-Gel P200 column were used to study enzyme-antibody interaction in broth culture containing either sucrose or glucose, and to localize ultrastructurally the enzyme on thin sections of bacteria using labelled and unlabelled antibodies. The antibody fractions had no effect on bacterial growth in broth culture. A strong inhibition of insoluble and soluble polysaccharides synthesis was obtained in batch culture after a 24 hours incubation of bacteria with rides synthesis was obtained in batch culture after a 24 hours incubation of bacteria with antibodies. The GTF was localized at different cellular levels on bacteria grown in glucose but the ultrastructural method failed to localize GTF in sucrose grown cells.
D000906:Antibodies; D003470:Culture Media; D005947:Glucose / Q000494:pharmacology*; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors* / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000037:antagonists & inhibitors; D013298:Streptococcus sanguis / Q000201:enzymology* / Q000648:ultrastructure; D013395:Sucrose / Q000494:pharmacology*
D000906:Antibodies; D003470:Culture Media; D011135:Polysaccharides, Bacterial; D013395:Sucrose; D005964:Glucosyltransferases; D005947:Glucose