[Inhibitory analysis of the functions of the oxidative metabolic systems of tumor cells in tissue culture].
As has been previously reported an increased intensity of light-induced green fluorescence is observed for some tumor cells. The present paper deals with the cause of this phenomenon, employing for this hepatoma cells of line HTC acted upon with 2,4-DNP, amytal and malonate. It has been shown that the light-induced increase in green fluorescence in cells is due to the oxidation of NADH-dehydrogenase, a mitochondrial flavine-containing enzyme, occurring at the time of fluorescence induction. The increased intensity of green fluorescence of flavoproteins in tumor cells is associated with an infringement in oxidation of NAD-dependent substrates in these, and with the activation of the reverse electron transport in the oxidative chain. The exciding light activates NADH-dehydrogenase and accelerates the translocation of reduced equivalents from this enzyme, which results in its oxidation, and thus--in the observed effect of increased intensity of green fluorescence.
D019297:2,4-Dinitrophenol; D000654:Amobarbital / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D046508:Culture Techniques; D004140:Dinitrophenols / Q000494:pharmacology; D005434:Flow Cytometry; D005453:Fluorescence; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008314:Malonates / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D014475:Uncoupling Agents / Q000494:pharmacology
D004140:Dinitrophenols; D008314:Malonates; D014475:Uncoupling Agents; D009249:NADP; D009245:NADH Dehydrogenase; D000654:Amobarbital; D019297:2,4-Dinitrophenol
Ingibitornyĭ analiz funktsional'nykh osobennosteĭ sistem okislitel'nogo metabolizma opukholevykh kletok v kul'ture tkaneĭ.