Differentiation of dicyclohexylcarbodiimide reactive sites of the ATPase complex bovine heart mitochondria.
1. In isolated bovine heart mitochondria, the 14C-labelled dicyclohexylcarbodiimide (DCCD) induced inhibition of the ATPase activity is accompanied by labelling of three polypeptides of Mx 9000, 16 000 and 33 000. Of these, only the 9000 polypeptide reacts with [14C]DCCD proportionally to the inhibitory effect, being saturated when the enzyme is maximally inhibited. 2. The 9000 and 16 000 polypeptides are extracted by neutral chloroform/methanol (2 : 1 v/v) while the 33 000 polypeptide remains in the non-extractable residue. No disaggregation of the polypeptides takes place during the extraction. 3. In the ATPase complex immunoprecipitated with antibody against F1, the 9000 and 16 000 polypeptides are present, but the 33 000 polypeptide is absent. 4. The results obtained indicate that the 33 000 polypeptide is not a component of the ATPase complex. As far as F0 is concerned, two types of the binding sites for DCCD were demonstrated, corresponding to the 9000 and 16 000 polypeptides. Their existence is explained by a non-random arrangement among individual monomers of the DCCD-binding protein.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D002234:Carbodiimides / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002417:Cattle; D004024:Dicyclohexylcarbodiimide / Q000378:metabolism*; D007700:Kinetics; D008929:Mitochondria, Heart / Q000378:metabolism*; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification
D002234:Carbodiimides; D002250:Carbon Radioisotopes; D002352:Carrier Proteins; D010455:Peptides; D004024:Dicyclohexylcarbodiimide; D000251:Adenosine Triphosphatases