Ethanol-induced increase in liver tryptophan oxygenase activity in the starved rat: evidence against tryptophan mediation.
A single oral dose of ethanol (4.0 g/kg) increased the activity of liver tryptophan oxygenase in starved male rats. The peak increase of 340% for the total activity and 400% for the holoenzyme activity occurred 6 hr after ethanol administration. At or after these peaks, the levels of tryptophan in plasma and brain but not in liver, decreased significantly. Plasma total tryptophan and brain tryptophan started to decrease significantly as early as 0.5-1.0 hr after the ethanol treatment, while the activity of liver tryptophan oxygenase was still at the control level. These findings suggest that not all the changes in tissue tryptophan concentrations seen after acute ethanol treatment are caused by increased liver tryptophan oxygenase activity. Prior to the increase in liver tryptophan oxygenase activity, an increase of 104 and 50% in plasma corticosterone and free tryptophan, respectively, were seen 15 min after ethanol treatment. However, the increase in liver tryptophan at this time appeared to be small (13%) and statistically insignificant. With tryptophan treatment, the initial peak levels of liver tryptophan and plasma free tryptophan required to stimulate an increase in tryptophan oxygenase activity were 170 times higher than those caused by ethanol. It was therefore concluded that increases in plasma and liver tryptophan after acute ethanol ingestion, probably mediated by the lipolytic action of ethanol, are too small to cause the increase in liver tryptophan oxygenase activity seen after ethanol administration. However, experiments with different corticosterone doses showed that ethanol-induced increases in plasma corticosterone concentrations are high enough to cause an increase in liver tryptophan oxygenase activity.
D000818:Animals; D001921:Brain / Q000378:metabolism; D003345:Corticosterone / Q000097:blood / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013217:Starvation / Q000201:enzymology; D014364:Tryptophan / Q000378:metabolism* / Q000502:physiology; D014366:Tryptophan Oxygenase / Q000096:biosynthesis*
D005230:Fatty Acids, Nonesterified; D000431:Ethanol; D014364:Tryptophan; D014366:Tryptophan Oxygenase; D003345:Corticosterone