Light rough endoplasmic reticulum membranes re-appear before heavy rough membranes after feeding starved MPC-11 cells.
In 48 hr starved MPC-11 cells smooth (S) endoplasmic reticulum (ER) membranes accounted for more than 80% of the total ER membrane fraction. After 2 1/2 hr feeding the amount of S membranes was reduced to below 55% while light rough (LR) membranes had increased from about 16% of the total to 42%. The percentage of heavy rough (HR) membranes only showed a minor increase during the incubation period. The results show that following an accumulation of S membranes in starved cells the LR membranes 're-appear' more rapidly than the HR membranes. This difference in behaviour provides further support to the concept that LR and HR membranes represent distinct domains of the ER system.
D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D002794:Choline / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000502:physiology* / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000502:physiology* / Q000648:ultrastructure; D007700:Kinetics; D051379:Mice; D010954:Plasmacytoma / Q000503:physiopathology; D013347:Subcellular Fractions / Q000502:physiology; D014529:Uridine / Q000378:metabolism
D002794:Choline; D014529:Uridine