Radioimmunoassay of cationic trypsin-like immunoreactivity in man.
The present paper describes the preparation of human cationic trypsin and of a stable and fully immunoreactive 125I-labelled tosyl-lysine chloromethyl ketone (TLCK) cationic trypsin by a modified chloramine-T method with a high specific radioactivity; the production of an avid and specific rabbit cationic trypsin antiserum; and a sensitive, precise, and specific radioimmunoassay method enabling measurements of fasting serum trypsin-like immunoreactivity (CTLI) in the low ng/ml range in normals; the significant rise in serum CTLI in patients with normal pancreatic exocrine secretion; and the absence of any rise in patients with severely reduced pancreatic exocrine secretion after intravenous injection of secretion; a markedly elevated fasting serum CTLI level in patients with acute pancreatitis; fasting CTLI in duodenal juice; and separation of CTLI in one minor and one major molecular component in fasting serum.
D004386:Duodenum / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007122:Immunoelectrophoresis; D007419:Intestinal Secretions / Q000032:analysis; D007457:Iodine Radioisotopes; D011863:Radioimmunoassay / Q000379:methods*; D014107:Tosyllysine Chloromethyl Ketone; D014357:Trypsin / Q000276:immunology / Q000378:metabolism*
D007457:Iodine Radioisotopes; D014107:Tosyllysine Chloromethyl Ketone; D014357:Trypsin