Hapten-coupled monoclonal anti-I-A antibodies provide a first signal for the induction of suppression.
A mechanism for induction of hapten-specific immune unresponsiveness is described that utilizes a hapten-conjugated monoclonal anti-Ia antibody to direct the in vivo immune response. Azobenzenearsonate- (ABA) coupled anti-I-Ak antibodies provide a potent first signal for the induction of nonresponsiveness to ABA in H-2k,a mice. The use of an anti-Ia antibody requires haplotype matching and may selectively direct the hapten signal, because other haptenated monoclonal antibodies are ineffective. There is an absolute requirement for a second signal to achieve in vivo suppression; the signal may be substituted by the generation of an allogeneic effect at the time of hapten presentation. We suggest that these methods allow for specific targeting of hapten-suppressive signals that differ considerably from other model systems of tolerance to haptenated immunoglobulins. The results are discussed in relation to a potential role for an Ia+ cell involved in the induction of regulating suppressor pathways.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D005260:Female; D006241:Haptens / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007108:Immune Tolerance; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D010132:p-Azobenzenearsonate / Q000276:immunology
D000911:Antibodies, Monoclonal; D006241:Haptens; D000949:Histocompatibility Antigens Class II; D010132:p-Azobenzenearsonate