A human T-cell antigen defined by xenoantiserum to Sézary leukemic cells: immunochemical and functional studies.
Xenoantiserum to Sézary cell leukemia cells (ASS) was developed by immunizing rabbits with those cells and was absorbed with human red cells, liver, tonsil B cells, and cultured Raji cells. This reagent reacted by immunofluorescence with virtually all human thymus and T cells. In the thymus, medullary cells reacted more strongly with ASS than did cortical thymocytes. When immunoprecipitates that formed between ASS and 125I-labeled lymphocyte surface glycoproteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, it was found that ASS precipitated a 72K molecular weight (MW) glycoprotein from T cells but not from B cells. On the one hand, it was shown by functional studies that T cells sensitive to the cytotoxic effect of ASS contained T cells that could aid the immunoglobulin synthesis of B cells induced by pokeweed mitogen. On the other hand, suppressor T cells induced by concanavalin A resided in those cells rather resistant to its cytotoxic effect. These data support the idea that the 72K MW glycoprotein on human thymus and T cells might be homologous to mouse Lyt-1 antigens.
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology; D001666:Binding Sites, Antibody; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology; D006680:HLA Antigens / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D008223:Lymphoma / Q000276:immunology* / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D011817:Rabbits; D012751:Sezary Syndrome / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000951:Antigens, Neoplasm; D000961:Antilymphocyte Serum; D006023:Glycoproteins; D006680:HLA Antigens; D000949:Histocompatibility Antigens Class II