A modified Lowry assay to measure levels of proteins lacking aromatic amino acid residues (e.g. metallothioneins).
A combination of two modifications to the Lowry protein assay is recommended for estimation of metallothionein (lacking aromatic amino acid residues and requiring reducing conditions for isolation). Serum thymic factor (STF), a decapeptide lacking aromatic amino acids, is recommended for use as a standard after allowance for the effects of short chain length on colour intensity development.
D008668:Metallothionein / Q000032:analysis*; D009842:Oligopeptides; D011506:Proteins / Q000032:analysis*; D014364:Tryptophan; D014443:Tyrosine
D009842:Oligopeptides; D011506:Proteins; D014443:Tyrosine; D014364:Tryptophan; D008668:Metallothionein