A simple, rapid and efficient isolation of erythrocyte Cu2Zn2-superoxide dismutase.
On the basis of the thermal stability of erythrocuprein (Cu2Zn2-superoxide dismutase) a rapid preparation technique was devised and successfully employed to isolate this protein. Partial heat-deterioration of the haemolysate and subsequent chromatography of the supernatant on DEAE-Sephacel and Sephadex G-75 yielded an electrophoretically homogeneous protein within a few days. The physicochemical properties and biochemical function were identical with those reported for Cu2Zn2-superoxide dismutases prepared by established methods.
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D003300:Copper / Q000032:analysis; D004912:Erythrocytes / Q000201:enzymology*; D006461:Hemolysis; D006358:Hot Temperature; D008722:Methods; D013482:Superoxide Dismutase / Q000097:blood / Q000302:isolation & purification*
D003300:Copper; D013482:Superoxide Dismutase