The beta-major and beta-minor globin genes in murine erythroleukemia cells replicate during the same early interval of the S phase.
The time of replication in S phase was determined for the 7.3 kb EcoRI segment containing the beta-major globin gene and the 14 kb EcoRI segment containing the beta-minor globin gene in a murine Friend erythroleukemia virus transformed (MEL) cell line. Cells were obtained from different intervals of S phase by centrifugal elutriation to avoid artifacts of chemical synchronization. Newly synthesized DNA from different parts of S phase were obtained by isolation of 5-bromouracil (BU) labelled DNA from these cells. The BU-DNA synthesized during four different intervals of S was transferred to diazobenzyloxymethyl (DBM) paper and hybridized with a beta-globin cDNA probe. Quantitation showed that both beta-globin segments were replicated in the first quarter of the S phase with no significant difference in their time of replication in this MEL cell line.
D000818:Animals; D001482:Base Composition; D002460:Cell Line; D005796:Genes; D005914:Globins / Q000235:genetics*; D007399:Interphase; D007942:Leukemia, Experimental / Q000378:metabolism* / Q000473:pathology; D051379:Mice
D005914:Globins