Effects of prophage Mu induction on expression of adjacent host genes.
The extent of induction and approximate amount of DNA replication of a Mu prophage carrying a gene for ampicillin resistance can be monitored by assaying the level of beta-lactamase. The expression of the lacZ gene adjacent to either end of an induced Mu prophage remains virtually unaffected, until late in the Mu lytic cycle, while Mu DNA is replicating and transposing.
D010583:Bacteriophage mu / Q000254:growth & development*; D004261:DNA Replication; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D007763:Lac Operon; D012097:Repressor Proteins; D014775:Virus Activation; D001616:beta-Galactosidase / Q000096:biosynthesis; D001618:beta-Lactamases / Q000096:biosynthesis
D004269:DNA, Bacterial; D012097:Repressor Proteins; D001616:beta-Galactosidase; D001618:beta-Lactamases