Assay of stearoyl-CoA synthesis in microsomes from normal and Trembler mouse sciatic nerves.
Using a new one-step technique which allows the characterization and a quantitative assay of long-chain acyl coenzyme A (acyl-CoA), the activity of the stearoyl-CoA synthetase was studied in the membrane fraction from sciatic nerves of normal and Trembler mice. A synthesis of acyl-CoAs was observed in both cases, but, whatever the experimental conditions, there was a two-fold increase of the specific activity of the ligase in the PNS of the Trembler mouse.
D000214:Acyl Coenzyme A / Q000096:biosynthesis* / Q000378:metabolism; D000818:Animals; D003066:Coenzyme A Ligases / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D008818:Mice, Neurologic Mutants; D008861:Microsomes / Q000201:enzymology*; D009186:Myelin Sheath / Q000201:enzymology*; D012584:Sciatic Nerve / Q000201:enzymology*
D000214:Acyl Coenzyme A; D005227:Fatty Acids; C017725:stearoyl-coenzyme A; D003066:Coenzyme A Ligases