Modulation of normal myelopoiesis in vitro by retinoic acid.
The effects of retinoic acid (RA) on the proliferation and differentiation of normal myeloid progenitor cells (CFU-C) were studied. In general, RA at 10(-10) to 10(-6) M enhanced primary myeloid colony formation in the presence of colony-stimulating factor(s). However, macrophage colony formation was strongly inhibited by RA. This may be related to the finding that RA is able to differentiate bipotential HL-60 cells into granulocytes but not into macrophages. Moreover, secondary colony formation was always suppressed by the addition of RA to the primary cultures. It means that self-renewal capacity of CFU-C was suppressed by RA. This finding suggests that normal myelopoiesis will be suppressed eventually by RA.
D001853:Bone Marrow / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D008264:Macrophages / Q000187:drug effects / Q000502:physiology; D014212:Tretinoin / Q000494:pharmacology*
D014212:Tretinoin