Structures of fucose-containing ceramide pentasaccharides from the plasma of blood group O Le(a-b-) nonsecretors.
A minor, Lec blood-group inactive ceramide pentasaccharide double band fraction has been isolated from the plasma of blood group O Le(a-b-) nonsecretors. The two purified glycolipids were analysed by NMR spectroscopy, mass spectrometry and combined gas chromatography-mass spectrometry. The following structures could be established: GlcNAc(beta 1----3)Gal(beta 1----4)Glc(beta 1----1)Cer (I); Gal(beta 1----4) [Fuc(alpha 1----3)]GlcNAc(beta 1----3)Gal(beta 1----4)Glc(beta 1----1)Cer (II). It must be concluded that at least part of the secretor gene-independent plasmatic H type 2 blood-group activity can be attributed to glycosphingolipid I, whereas substance II, originally detected in cancerous tissue, also occurs in the plasma of healthy individuals.
D000017:ABO Blood-Group System; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D005643:Fucose / Q000032:analysis*; D006028:Glycosphingolipids / Q000097:blood*; D006801:Humans; D007983:Lewis Blood Group Antigens; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009844:Oligosaccharides / Q000302:isolation & purification*
D000017:ABO Blood-Group System; D006028:Glycosphingolipids; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides; D005643:Fucose