Influence of thymosin and the supernatant of cultured mouse thymus reticuloepithelial cell on mouse immune responses.
Effects of the humoral factor extracted from the calf thymus (thymosin: Goldstein et al.) and the supernatant of cultured mouse thymus reticuloepithelial cell (TRC) on immunological functions of the mouse lymphocytes were studied, and the following conclusions were drawn: Thymosin treatment did not alter the capacity to form anti-SRBC antibody in adult mice which were lethally irradiated and reconsituted with bone marrow cells. When the spleen cells from JCL-ICR mice thymectomized within 24 hr after birth were cultured with thymosin or supernatant of cultured ICR-TRC, the Thy 1 positive cells were increased. A significant increase in the mitogenic (PHA, Con A) reactivity of BALB/C spleen cells was observed when these cells were preincubated in medium containing thymosin for 24 hr. The NZB spleen cells, however, exhibited spleen cells showed a slightly potentiated response only to Con A. Similarly, thymosin enhanced the MLC reaction of the spleen cells from BALB/C mice with preincubation for 2 hr, but not enhanced that from NZB mice.
D000818:Animals; D000917:Antibody Formation; D002478:Cells, Cultured; D037102:Lectins / Q000494:pharmacology; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008814:Mice, Inbred NZB; D012157:Mononuclear Phagocyte System / Q000166:cytology / Q000276:immunology*; D013045:Species Specificity; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013947:Thymosin / Q000494:pharmacology*; D013951:Thymus Hormones / Q000494:pharmacology*
D037102:Lectins; D013951:Thymus Hormones; D013947:Thymosin