Interference of cyclosporin with lymphocyte activation: blockage of the mitogen-induced increases of lysosomal and mitochondrial activities.
Mouse lymphocytes were activated by a mitogenic dose of concanavalin A and analysed by flow cytometry to monitor the increases of mitochondrial activity (using rhodamine 123 as probe) and of lysosomal activity (using euchrysin [acridine orange] as probe). Cyclosporin A-treated lymphocytes were not capable of responding to concanavalin A in the same way as untreated lymphocytes: both the increased uptakes of rhodamine 123 by mitochondria and of acridine orange by lysosomes were strongly diminished, though not abolished. Cyclosporin may thus interfere at a step of activation prior or concurrent to those early changes of lymphocyte physiology. It looks like that it allows mitogen-activated cells to go through part of the mitochondrial maturation which precedes initiation of nuclear DNA synthesis, after which the cells remain blocked at that incomplete maturation level.
D000165:Acridine Orange / Q000378:metabolism; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology*; D003524:Cyclosporins / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000378:metabolism*; D020112:Rhodamine 123; D012235:Rhodamines / Q000378:metabolism
D003524:Cyclosporins; D012235:Rhodamines; D003208:Concanavalin A; D020112:Rhodamine 123; D000165:Acridine Orange