Failure to detect delta 5-3 beta-hydroxysteroid oxidoreductase activity in the preimplantation rabbit embryo.
Preimplantation rabbit embryos were incubated with pregnenolone and dehydroisoandrosterone under conditions which gave formazan precipitation by the histochemical technique. The metabolic fate of the labeled steroids was assessed simultaneously. There was no concomitant transformation of pregnenolone to progesterone and dehydroisoandrosterone was not transformed to androstenedione. It is concluded that the formazan precipitation is coupled with an "activity" other than delta 5-3 beta-hydroxysteroid oxidoreductase.
D015096:3-Hydroxysteroid Dehydrogenases / Q000032:analysis*; D000818:Animals; D001755:Blastocyst / Q000201:enzymology*; D003687:Dehydroepiandrosterone / Q000378:metabolism; D005260:Female; D011247:Pregnancy; D011284:Pregnenolone / Q000378:metabolism; D011375:Progesterone Reductase / Q000032:analysis*; D011817:Rabbits; D011865:Radioisotope Dilution Technique; D014316:Tritium
D014316:Tritium; D003687:Dehydroepiandrosterone; D011284:Pregnenolone; D015096:3-Hydroxysteroid Dehydrogenases; D011375:Progesterone Reductase