[Determination of glycosylated albumin in controls and in diabetics by affinity chromatography with phenylboronic acid].
The determination of glycosylated albumin was performed in 19 diabetic patients and in 16 control subjects, by means of chromatographic separation on columns of phenilboronic acid, immobilized by agarose gel. The interference with free glucose was eliminated by serum gel filtration on sephadex G 25 columns. The results demonstrated that glycosylated albumin values, obtained by affinity chromatography, discriminate diabetic by non-diabetic subjects and significantly correlate with glycosylated proteins levels, taken by colorimetric method with thiobarbituric acid. The chromatographic technique resulted in a more simplified way than colorimetric method and has shown to be sensitive to significant increases in "in vitro" glycosylation. Moreover it lasts less than the other technique and its variation coefficient is extremely low. In conclusion it represents a progress in the routine determination of glycosylated albumin.
D000328:Adult; D000368:Aged; D002846:Chromatography, Affinity / Q000379:methods; D003124:Colorimetry; D003920:Diabetes Mellitus / Q000097:blood*; D005260:Female; D017127:Glycation End Products, Advanced; D006801:Humans; D008297:Male; D008875:Middle Aged; D012685:Sepharose / Q000031:analogs & derivatives; D012709:Serum Albumin / Q000032:analysis*; D000093423:Glycated Serum Albumin
D017127:Glycation End Products, Advanced; D012709:Serum Albumin; C035597:phenylboronic acid-sepharose; D012685:Sepharose; D000093423:Glycated Serum Albumin
Determinazione dell' albumina glicosilata, in soggetti di controllo e in diabetici, mediante cromatografia di affinita' con acido fenilboronico.