Some properties of the interstrand crosslinks in depurinated DNA.
The interstrand crosslinks that appear in stored depurinated DNA interfere with the counting of apurinic sites and strand breaks by sucrose gradient analysis. They could not be cleaved at acid or alkaline pH, or by treatment with methoxyamine.
D004279:DNA, Viral / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D006898:Hydroxylamines / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D011687:Purines / Q000032:analysis; D013604:T-Phages / Q000235:genetics
D004279:DNA, Viral; D006898:Hydroxylamines; D011687:Purines; C005214:methoxyamine