Chinese hamster cell mutants with defective endocytosis of low-density lipoprotein contain altered fatty acid composition in the membrane.
Chinese hamster V79 cell mutants resistant to compactin (ML236B), a specific inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A reductase, are defective in endocytosis of low-density lipoprotein (1). Two resistant clones, MF-2 and MF-3, differ in lipid composition from the parental V79 strain. In both the total cells and membrane fraction, the ratio of palmitoleic acid (16:1)/palmitic acid (16:0) is 0.4-0.5 in MF-2 and 1.7-1.8 in MF-3 while that in V79 is 0.2-0.3. By contrast, a hybrid clone between V79 and MF-3 shows a ratio of palmitoleic acid to palmitic acid very similar to that of V79. The synthesis of palmitoleic acid from acetate in the resistant clone is higher than in V79.
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000818:Animals; D002250:Carbon Radioisotopes; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002999:Clone Cells; D006224:Cricetinae; D003412:Cricetulus; D004705:Endocytosis; D005227:Fatty Acids / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008168:Lung; D008563:Membrane Lipids / Q000378:metabolism*; D009154:Mutation
D000085:Acetates; D002250:Carbon Radioisotopes; D005227:Fatty Acids; D008077:Lipoproteins, LDL; D008563:Membrane Lipids; D019342:Acetic Acid