GLC analysis following oxidation of phenytoin. An inexpensive method for routine determination of the drug in plasma.
Phenytoin (5,5-diphenyl-2,4-imidazolidinedione) is readily oxidized to benzophenone. Using spiro(fluorene-9,4-imidazolidine)-2',5'-dione or 5-(4'-methoxyphenyl)-5-phenyl-2,4-imidazolidinedione as internal markers the oxidation product can be analyzed by GLC, providing an analytical method for plasma phenytoin which removes the need for preliminary extraction of the drug.
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D010084:Oxidation-Reduction; D010672:Phenytoin / Q000097:blood*; D011817:Rabbits
D010672:Phenytoin