Regulation of phosphatidylcholine biosynthesis by the methylation pathway in rat pituitary gland.
Rat pituitary extracts catalyze methylation of phosphatidylethanolamine to phosphatidylcholine using S-adenosyl-L-methionine as the methyl donor. In vitro incubation of hemipituitaries with 1 mM 2-methylaminoethanol led to a dose dependent decrease in phosphatidylethanolamine methyltransferase activity as well as the incorporation of the radioactivity from [3H-methyl]-L-methionine into phosphatidyl-choline. The inhibitory effect of 2-methylaminoethanol was selective for phospholipids methylation since protein carboxymethyltransferase and catechol-o-methyltransferase activities were not affected.
D000818:Animals; D002394:Catechol O-Methyltransferase / Q000378:metabolism; D004983:Ethanolamines / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008715:Methionine / Q000378:metabolism; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D050918:Phosphatidylethanolamine N-Methyltransferase; D010902:Pituitary Gland / Q000201:enzymology / Q000378:metabolism*; D014176:Protein Biosynthesis; D011497:Protein O-Methyltransferase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D004983:Ethanolamines; D010713:Phosphatidylcholines; D008715:Methionine; D008780:Methyltransferases; D011497:Protein O-Methyltransferase; D050918:Phosphatidylethanolamine N-Methyltransferase; D002394:Catechol O-Methyltransferase; C017500:N-methylaminoethanol