Increased adherent suppressor cell activity in allergic children.
Monocyte regulation of IgE biosynthesis was studied in 20 asthmatic children and 20 healthy normals. The monocytes (MN's) were separated from mononuclear cells (MNC's) by plastic petri dish adherence and part of them were activated by lippopolysaccharide (LPS, 2 micrograms/ml for 1 hour). The results showed that (1) MNCs of patients produced much more IgE than did the MNC's of normals, (2) monocyte-depletion resulted in decreased IgE production of patients' MNC's but not normals' MNC's (3) monocytes of patients could enhance the IgE-synthesizing capability of T cell activity in IgE biosynthesis in both allergic patients and normals. It is, therefore, concluded that there exist in allergic patients suppressor adherent cells which suppress the regulatory T cell function and may play a certain role in the immunopathogenesis of augmented IgE production in atopic individuals.
D000293:Adolescent; D000328:Adult; D001249:Asthma / Q000276:immunology*; D001772:Blood Cell Count; D002448:Cell Adhesion; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology; D009000:Monocytes; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D008070:Lipopolysaccharides; D007073:Immunoglobulin E