Examination of the subcellular distribution of tripeptide aminopeptidase and evaluation of its clinical usefulness in human serum.
Following electrophoretic separation, we assayed tripeptide aminopeptidase (EC 3.4.11.4) in human tissues and sera free of interferences by other aminopeptidases. Tripeptide aminopeptidase is distributed in various human tissues, with the highest activity observed in liver and lymphocytes. The highest specific activity of the enzyme was observed in the soluble fraction prepared from liver, and 65% of the enzyme activity in the original homogenate was recovered in this fraction. Cancerous and fetal tissues showed lower enzyme activities than normal adult tissues. Elevations of tripeptide aminopeptidase were observed in sera of patients with liver disorders, leukemias, and autoimmune diseases.
D000626:Aminopeptidases / Q000032:analysis* / Q000097:blood; D001327:Autoimmune Diseases / Q000201:enzymology; D003095:Collagen Diseases / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology*; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D005260:Female; D005333:Fetus / Q000201:enzymology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D007931:Leucyl Aminopeptidase / Q000032:analysis; D008099:Liver / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology; D008297:Male; D009369:Neoplasms / Q000201:enzymology; D014018:Tissue Distribution
D007770:L-Lactate Dehydrogenase; D000626:Aminopeptidases; D007931:Leucyl Aminopeptidase; C026045:tripeptide aminopeptidase; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases