A simple in vitro technique to measure the release of endogenous dopamine and dihydroxyphenylacetic acid from striatal tissue using high performance liquid chromatography with electrochemical detection.
An easily constructed, inexpensive and simple system is described for the superfusion of neural tissue. To characterize the system, the dynamics of endogenous dopamine and dihydroxyphenylacetic acid release from striatal tissue were studied before and after exposure to potassium, amphetamine or cocaine.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism*; D000661:Amphetamine / Q000494:pharmacology; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003042:Cocaine / Q000494:pharmacology; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D004563:Electrochemistry; D008297:Male; D010648:Phenylacetates / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D013696:Temperature
D010648:Phenylacetates; D015102:3,4-Dihydroxyphenylacetic Acid; D000661:Amphetamine; D003042:Cocaine; D011188:Potassium; D004298:Dopamine