Identification by cross-linking of a neuronal acceptor protein for dendrotoxin, a convulsant polypeptide.
Dendrotoxin, a lijow molecular weight protein from the venom of Dendroaspis angusticeps, is known to be a potent convulsant that attenuates one type of voltage-sensitive K+ channel in guinea-pig hippocampus. A biologically active preparation of 125I-labelled dendrotoxin has been cross-linked to its high-affinity protein acceptor in synaptic plasma membranes from rat cerebral cortex. On SDS gel electrophoresis, a complex with a Mr of 72,000 was observed which, assuming one toxin molecule is attached, yields an apparent size of 65,000 for this subunit of the acceptor. Unlike dendrotoxin, low concentrations of beta-bungarotoxin, another pre-synaptically active toxin, do not inhibit its labelling.
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D003292:Convulsants; D003432:Cross-Linking Reagents; D004546:Elapid Venoms / Q000378:metabolism* / Q000633:toxicity; D007457:Iodine Radioisotopes; D007700:Kinetics; D008970:Molecular Weight; D015221:Potassium Channels; D051381:Rats; D011950:Receptors, Cholinergic / Q000302:isolation & purification / Q000378:metabolism*; D013570:Synaptic Membranes / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism*
D003292:Convulsants; D003432:Cross-Linking Reagents; D004546:Elapid Venoms; D007457:Iodine Radioisotopes; D015221:Potassium Channels; D011950:Receptors, Cholinergic; C042467:dendrotoxin receptor; C020273:dendrotoxin