Ia-dependent interleukin 2 production in syngeneic cellular interactions.
In the murine syngeneic mixed lymphocyte response (SMLR), the co-culture of nylon wool-nonadherent spleen cells or young (4 to 6-day-old) thymocytes with syngeneic nylon wool-adherent adult spleen cells, results in proliferation and Interleukin 2 (IL 2) production. In both the thymus and spleen SMLR, proliferation and IL 2 production are the function of Lyt-1+2-3- (Lyt-1) responder cells and require the expression of IA (IB) and/or IE cell surface antigens on the stimulator cell. IL 2 production in both spleen and thymus SMLR is independent of heterologous serum factors (i.e., fetal calf serum), which, however, influences the magnitude of the in vitro proliferation. The IL 2-producing cell in spleen is capable of self-renewal in the absence of the thymus; it is detectable 6 to 7 mo after adult thymectomy. These results suggest the in vitro SMLR may measure a cellular interaction between the Lyt-1 subset of T cells and self Ia antigens on macrophage-like cells that produces IL 2 in an antigen-independent fashion. This interaction may have physiologic significance in the expression and regulation of T cell function.
D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2 / Q000096:biosynthesis*; D007518:Isoantibodies / Q000276:immunology; D008211:Lymphocyte Cooperation; D007959:Lymphocyte Culture Test, Mixed; D008222:Lymphokines / Q000096:biosynthesis*; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008819:Mice, Nude; D013154:Spleen / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism; D013934:Thymectomy
D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D007518:Isoantibodies; D008222:Lymphokines; C029805:Lyt antibodies