Monoclonal antibody to glutamate dehydrogenase isolation and characterization.
This paper describes the isolation and characterization of a monoclonal antibody to bovine liver glutamate dehydrogenase (GDH). Monoclonal antibody is mouse immunoglobulin subclass IgG2a and reacts strongly with the antigen in an enzyme-linked immunosorbent assay (ELISA). Its specificity was determined by an antigen binding assay and by Western blotting. Potential uses and possible applications are discussed.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005969:Glutamate Dehydrogenase / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008930:Mitochondria, Liver / Q000201:enzymology; D008970:Molecular Weight
D000911:Antibodies, Monoclonal; D005969:Glutamate Dehydrogenase