Effect of skin allograft immunization on the mixed leukocyte reaction of the clawed frog, Xenopus laevis.
One-way mixed leukocyte reactions (MLRs) of responder spleen cells from frogs (Xenopus laevis) that had rejected skin grafts were examined. Maximum and median stimulation indices (SIs) of cultures that contained responder cells from the graft recipient and stimulator cells from the graft donor were significantly greater than the peak and median SIs of cultures in which the responder splenocytes were from control, nonimmune animals. Significant stimulation was also recorded earlier (day 2 of culture) in more cultures involving immune responder cells than in control cultures. This earlier and greater proliferation in MLR was antigen specific.
D000818:Animals; D006084:Graft Rejection; D007700:Kinetics; D007959:Lymphocyte Culture Test, Mixed; D016038:Skin Transplantation; D013154:Spleen / Q000276:immunology*; D013997:Time Factors; D014982:Xenopus laevis / Q000276:immunology*