Cytotoxicity of cultured mouse spleen cells against natural killer-sensitive target cells. Characterization of the effector cells.
Killer cells with natural killer (NK) cell-like target selectivity were generated when C3H or C57BL/6 mouse spleen cells were cultured in vitro. Compared with NK cells, the in vitro-generated killer cells with NK-like reactivity were slightly adherent to nylon wool fibers, a little more sensitive to anti-Thyl.2 antibody plus complement and almost entirely resistant to cytotoxic treatment with anti-asialo GM1 antiserum which is selectively cytotoxic to NK cells. The culture of NK-depleted spleen cells yielded a lower degree of NK-like cytotoxicity, thus it seems likely that NK cells changed to NK-like killer cells in vitro. Competitive inhibition studies suggested that the in vitro-generated NK-like cells were different from alloantigen-reactive killer T cells. The above results, therefore, might suggest that in vitro-induced NK-like cells do not belong to any known subset of killer cells.
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D005677:G(M1) Ganglioside; D006028:Glycosphingolipids / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008223:Lymphoma / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D010586:Phagocytes / Q000276:immunology; D013154:Spleen / Q000276:immunology*; D013997:Time Factors
D006028:Glycosphingolipids; D007106:Immune Sera; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside