Transmembrane control of laminin, lectin receptors and surface villi in teratocarcinoma-derived endodermal cells.
Distribution of laminin on the surface of teratocarcinoma-derived parietal endoderm cells was studied by immuno-histochemical staining of the fixed specimen using affinity-purified anti-laminin antibody. Laminin was distributed on the basal surface of the cells, while treatment either with colchicine or with cytochalasin D (CD) resulted in a severely polarized distribution; laminin was seen only at one end of the cell. Treatment with both the reagents did not cause the severe polarization. Receptors for lectins and cell surface villi were polarized by treatment with CD but not by treatment with colchicine. These results suggest that laminin--or its cell surface receptor--is linked to both microfilament and microtubules and that the mode of transmembrane control for laminin is different from certain other cell surface components of the cells.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D003078:Colchicine / Q000494:pharmacology; D007797:Laminin / Q000378:metabolism*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000187:drug effects / Q000648:ultrastructure*; D011975:Receptors, Mitogen / Q000378:metabolism*; D013724:Teratoma / Q000473:pathology / Q000503:physiopathology* / Q000648:ultrastructure
D007797:Laminin; D011975:Receptors, Mitogen; D003078:Colchicine