Chicken liver non-histone protein fraction repressing DNA transcription in vitro.
A chicken liver non-histone protein fraction repressing DNA transcription in vitro was isolated using ultrasound shearing followed by precipitation with divalent cations. It was characterized by SDS-polyacrylamide electrophoresis and isoelectric focusing. This inhibitory protein fraction showed strong affinity to DNA and represented non-histone proteins with main bands of mol. wt about 35000 and 56000 and isoelectric points about 5.7 and 6.2. The inhibitory non-histone protein fraction seems to repress DNA transcription by interacting with DNA template rather than with RNA polymerase.
D000818:Animals; D002645:Chickens; D002868:Chromosomal Proteins, Non-Histone / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007526:Isoelectric Point; D008099:Liver / Q000032:analysis*; D008970:Molecular Weight; D014158:Transcription, Genetic / Q000187:drug effects*; D014465:Ultrasonics
D002868:Chromosomal Proteins, Non-Histone; D004247:DNA