Alkali-labile sites in biologically active DNA: comparison of radiation induced potential breaks and apurinic sites.
Gamma-ray induced alkali-labile sites, which are converted into breaks by alkali or heat, are compared with apurinic sites in both single-stranded and phi X174RF-DNA. It is shown that the kinetics of the conversion into breaks are completely different for both sites. The conclusion is: the gamma-ray induced alkali-labile sites are not identical with apurinic or/and apyrimidinic sites.
D000468:Alkalies; D010584:Bacteriophage phi X 174; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D004279:DNA, Viral / Q000528:radiation effects*; D005720:Gamma Rays; D011687:Purines; D012972:Sodium Hydroxide; D013997:Time Factors
D000468:Alkalies; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D011687:Purines; D012972:Sodium Hydroxide