[Methodical investigations for selection of a histochemical routine-method of differentiating fibre types (author's transl)].
1. Transverse sections of two muscles of 20 pigs and 20 laboratory mice were studied methodically to ascertain a histochemical technique of differentiating fibre types suitable for routine-investigations. 2. The best results of four applied histochemical methods were obtained by the reaction for reduced disphosphopyridine nucleotide tetrazolium reductase (DPNH-TR). The comparison of the methods based on valuation-criterions as quality of reactions, expenditure of time and cost and requirements to the user. 3. The calculation of the variance-components showed for the evaluation of two samples (pigs) and one transverse section of muscle (mouse) per animal a good repeatability of the quantitative parameters of muscle fibre type composition.
D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D006651:Histocytochemistry / Q000379:methods*; D008055:Lipids / Q000032:analysis; D051379:Mice; D009132:Muscles / Q000032:analysis* / Q000201:enzymology; D009246:NADH Tetrazolium Reductase; D013385:Succinate Dehydrogenase / Q000032:analysis; D013552:Swine
D008055:Lipids; D013385:Succinate Dehydrogenase; D009246:NADH Tetrazolium Reductase; D000251:Adenosine Triphosphatases
Methodische Untersuchungen zur Auswahl einer histochemischen Routinemethode für die Differenzierung von Muskelfasertypen.