Enhanced radiosensitivity for the induction of translocations in mouse stem cell spermatogonia following treatment with cyclophosphamide or adriamycin.
The effects of pretreatment of mouse spermatogonial stem cells with cyclophosphamide (100 and 200 mg/kg) and adriamycin (2.5, 5 and 7 mg/kg) on the induction of chromosomal translocations by high doses (800 or 900 rad) of X-rays applied 24 h later, were studied by spermatocyte analysis. The results indicate that both compounds were able to alter the chromosomal radiosensitivity of surviving stem cells. It is concluded that depletion of differentiating and differentiated spermatogonia is sufficient for triggering stem cells into a more radiosensitive phase.
D000818:Animals; D003520:Cyclophosphamide / Q000633:toxicity*; D004317:Doxorubicin / Q000633:toxicity*; D008297:Male; D051379:Mice; D009153:Mutagens; D011838:Radiation-Sensitizing Agents; D013093:Spermatogonia / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects*; D013094:Spermatozoa / Q000528:radiation effects*; D014178:Translocation, Genetic / Q000187:drug effects / Q000528:radiation effects*
D009153:Mutagens; D011838:Radiation-Sensitizing Agents; D004317:Doxorubicin; D003520:Cyclophosphamide