Polymorphism of sarcoplasmic-reticulum adenosine triphosphatase of rabbit skeletal muscle.
Antibody was raised in chickens against purified sarcoplasmic-reticulum Ca2+-activated ATPase (Ca2+-ATPase). The immunological relationship between the Ca2+-ATPase of fast-muscle and slow-muscle sarcoplasmic reticulum was investigated by a one-step and a two-step competitive enzyme-linked immunosorbent assay (ELISA). The results show marked antigenic differences between the membrane-bound Ca2+-ATPase of the sarcoplasmic-reticulum vesicles from fast muscle and slow muscle, beside differences in the membrane content of ATPase protein.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000252:Calcium-Transporting ATPases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D009126:Muscle Relaxation; D009132:Muscles / Q000201:enzymology / Q000276:immunology*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000201:enzymology / Q000276:immunology*
D000906:Antibodies; D000252:Calcium-Transporting ATPases