Conformational flexibility of poly (dG-m5dC) under very low salt conditions.
The methylated DNA polymer poly (dG-m5dC) which exhibits a B helical conformation in solutions containing 20 mM NaCl, undergoes a gradual and reversible transition to the Z conformation as the NaCl concentration is lowered. The midpoint of this transition occurs around 5-6 mM NaCl. The conformational flexibility of this polymer at such low NaCl concentrations opens up the possibility of studying the effects of other perturbants with negligible interference from salt concentration effects.
D002942:Circular Dichroism; D007038:Hypotonic Solutions; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D011089:Polydeoxyribonucleotides; D012965:Sodium Chloride / Q000494:pharmacology*
D007038:Hypotonic Solutions; D011089:Polydeoxyribonucleotides; D012965:Sodium Chloride; C029988:poly(dG-m(5)dC).poly(dG-m(5)dC)