Identification of wild-type or mutant alleles of bacterial genes cloned on a bacteriophage lambda vector: isolation of uvrC(am) and other mutants.
We have identified lambda transducing bacteriophages carrying deoxyribonucleic acid repair or recombination genes of Escherichia coli K-12 by their ability to infect and express their bacterial genes in mutant cells in an agar overlay. This technique has been used to recognize transducing phages carrying uvrC+, ssb+, and other genes and to isolate phages carrying mutant alleles unable to complement ssb or uvrC cells. Several uvrC mutations were obtained which were suppressor sensitive.
D010582:Bacteriophage lambda / Q000235:genetics*; D003001:Cloning, Molecular; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005816:Genetic Complementation Test; D009154:Mutation; D011995:Recombination, Genetic; D014161:Transduction, Genetic; D014466:Ultraviolet Rays; D010948:Viral Plaque Assay