Induced hydrosalpinges in rabbits: comparison of hysterosalpingographic media and development of an animal model.
Experiments were conducted in rabbits to test the effect of commonly used hysterosalpingographic media on tubal structure and function. A model of hydrosalpinx was created by ligating both ends of the oviduct, and the lumen was filled with test solutions. Three groups of animals were studied. Group 1 rabbits (operated controls) received Hanks' balanced salt solution or no solution. In the second group (unoperated controls), no solution or occlusive ligature was applied. Group 3 received either Ethiodol (Savage Laboratories, Missouri City, TX), an oil-soluble contrast medium (OSCM), or Sinografin (E.R. Squibb & Sons, Princeton, NJ), a water-soluble contrast medium (WSCM). Histopathologic changes in the endosalpinx were moderate in the doubly ligated oviducts, and no difference was found between the effects of OSCM and WSCM in group 3 or between groups 1 and 3. Ciliary transport rate on the ampullary endosalpinx of group 1 was 0.05 mm/second, i.e., half the normal rate in the unoperated controls (P less than 0.001) and OSCM-filled oviducts (P less than 0.01). The rate in WSCM oviducts was 0.01 mm/second, significantly slower than in the OSCM-filled oviducts (P less than 0.05).
D000818:Animals; D003287:Contrast Media / Q000633:toxicity*; D004195:Disease Models, Animal; D005184:Fallopian Tube Diseases / Q000000981:diagnostic imaging* / Q000473:pathology; D005260:Female; D007047:Hysterosalpingography / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D009092:Mucous Membrane / Q000187:drug effects / Q000648:ultrastructure; D010057:Oviducts / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000648:ultrastructure; D011817:Rabbits
D003287:Contrast Media