Effect of halomethanes on intracellular calcium distribution in hepatocytes.
Exposure of isolated rat hepatocytes to hepatotoxic halomethanes results in a 40-60% decrease in intracellular Ca2+ content. The order of halomethane potency (CBrCl3 CCl4 CHCl3) suggests that this effect requires halomethane metabolism by the hepatic mixed function oxidase system. Although the Ca2+ sequestering ability of the endoplasmic reticulum is destroyed by CBrCl3 and CCl4, it appears that much of the Ca2+ lost from the cell is mitochondrial in origin. Paradoxically, saturating concentrations of CCl4 cause a marked increase in cell Ca2+. CCl4 also causes an acute increase in cytoplasmic free Ca2+ (from about 60 nM to about 90 nM), but this effect does not appear to require CCl4 metabolism and is probably a result of direct action of CCl4 on the plasma membrane.
D000634:Aminoquinolines; D000818:Animals; D001975:Bromotrichloromethane / Q000633:toxicity*; D002118:Calcium / Q000378:metabolism*; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism*; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D002451:Cell Compartmentation; D002725:Chloroform / Q000031:analogs & derivatives* / Q000633:toxicity*; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D051381:Rats
D000634:Aminoquinolines; D005456:Fluorescent Dyes; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D002725:Chloroform; C035738:Quin2-acetoxymethyl ester; D001975:Bromotrichloromethane; D002118:Calcium