Induction of TNF-like factor by murine macrophage-like cell line J774.1 on treatment with Sarcophaga lectin.
On stimulation with Sarcophaga lectin, the mouse macrophage-like cell line J774.1 secreted a factor like the tumor necrosis factor (TNF) into the culture medium. This factor was a protein with a molecular weight of 40000-45000, and was cytotoxic to L-929 cells, but not to normal embryonic fibroblasts. This factor was effective on both the ascites form and solid form of sarcoma 180 transplanted into ICR mice.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004355:Drug Stability; D006023:Glycoproteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000633:toxicity; D006131:Growth Inhibitors / Q000096:biosynthesis*; D019476:Insect Proteins; D007739:L Cells / Q000166:cytology; D037102:Lectins / Q000494:pharmacology*; D037181:Lectins, C-Type; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D012510:Sarcoma 180 / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha
C043030:20kDa lectin protein, Sarcophaga; D006023:Glycoproteins; D006131:Growth Inhibitors; D019476:Insect Proteins; D037102:Lectins; D037181:Lectins, C-Type; D014409:Tumor Necrosis Factor-alpha