Phospholipase C of an oral strain of Propionibacterium acnes purification and partial characterization.
Phospholipase C of the oral Propionibacterium acnes strain D 7 was purified from culture supernatants and partially characterized. The molecular weight was found to be 32.000 and the optimum pH was situated between 7.0 to 8.0. This nonhemolytic enzyme hydrolyzed relative intensively acidic glycerophospholipids and produced 1,2-diglyceride from phosphatidyl choline.
D002855:Chromatography, Thin Layer; D003773:Dental Plaque / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010740:Phospholipases / Q000302:isolation & purification*; D011425:Propionibacterium acnes / Q000201:enzymology*; D013379:Substrate Specificity; D010738:Type C Phospholipases / Q000302:isolation & purification* / Q000378:metabolism
D010740:Phospholipases; D010738:Type C Phospholipases