[Purification of prostatic acid phosphatase from human seminal plasma].
Prostatic acid phosphatase has been isolated from human seminal plasma. The purification method utilizes gel filtration on Sephadex G100, ammonium sulfate precipitation and a series of chromatographical steps including concanavalin A Sepharose 4B, anion exchange and gel filtration chromatography. The final product appears homogenous when analyzed by gel filtration on Sephadex G100. It gives one major band on SDS polyacrylamide gels. The specific activity is similar to that obtained by other purification schemes. The yield of the method described above has allowed to set up a sensitive radioimmunoassay of prostatic acid phosphatase.
D000135:Acid Phosphatase / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008832:Microchemistry; D008970:Molecular Weight; D011467:Prostate / Q000201:enzymology*; D011863:Radioimmunoassay / Q000379:methods; D012661:Semen / Q000201:enzymology*
D000135:Acid Phosphatase
Purification de la phosphatase acide prostatique à partir de plasma séminal humain.