Use of gene cloning to determine polarity of an operon: genes carAB of Escherichia coli.
A gene-cloning approach was used to determine the transcription polarity of the carbamoylphosphate operon (carAB) of Escherichia coli. In agreement with the accompanying paper (J. Bacteriol. 143:914-920, 1980), our results lead to the conclusion that carA is the proximal gene of the carAB operon.
D010582:Bacteriophage lambda / Q000235:genetics; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D008025:Ligases / Q000235:genetics*; D009876:Operon; D010957:Plasmids; D014158:Transcription, Genetic
D008025:Ligases; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)