Suppressor factor from a T cell hybrid inhibits delayed-type hypersensitivity responses to azobenzenearsonate.
By using polyethylene glycol 1540, BW5147 AKR T lymphoma cells were fused with splenocytes from A/J mice treated so as to induce suppressor T cells specific for azobenzenearsonate (ABA). Of 576 microwells originally seeded, 132 demonstrated growing cell clones, 4 of which produced an ABA-binding supernatant factor. When tested in vivo for suppression of delayed-type hypersensitivity to ABA, two of these cell lines, A4 and F12, were shown to produce suppressive supernatant factors. Fluorescence analysis of the F12 cells with appropriate antisera demonstrated this T cell hybrid to be Thy 1.2+, Lyt 1+,2-, and surface immunoglobulin negative, the surface marker phenotype of conventional ABA-specific suppressor T cells. This cloned suppressor cell line, F12, produces a culture supernatant factor that is suppressive at dilutions up to 1:100 and has provided material for genetic and immunochemical analysis.
D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology*; D002460:Cell Line; D002999:Clone Cells; D005260:Female; D006825:Hybridomas / Q000187:drug effects / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007166:Immunosuppressive Agents / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D009930:Organic Chemicals; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology*; D010132:p-Azobenzenearsonate / Q000494:pharmacology*
D001391:Azo Compounds; D007166:Immunosuppressive Agents; D009930:Organic Chemicals; C023051:suppressive factor of allergy; D010132:p-Azobenzenearsonate