[Work of the active transport system of organic acids in the kidney proximal tubules of rats with compensatory hypertrophy].
The active transport of organic acids in proximal renal tubules of the Campbell, Wistar and random-bred rats was studied by contact microfluorometry with anion dye fluorescein as a marker, on 1, 2, 3, 5, 10, 30 and 90 days after a unilateral nephrectomy. Sham-operated rats were used as a control. Transport was investigated under certain conditions when fluorescein was transported by Na-dependent (at 30 degrees C) or Na-independent (at 20 degrees C) systems. It is shown that fluorescein accumulation in renal tubules of operated random-bred rats at 30 degrees C is (if compared to the corresponding control) 81, 105 and 75% on 1, 2 and 3-10 days, resp., and about 90% on 30 and 90 days. Fluorescein accumulation at 20 degrees C in all investigated rats was about 90% of the corresponding control. A specific poison of Na+, K+-ATPase = ouabain inhibited fluorescein accumulation on day 1 after sham-operation by 70% to control, but there was no inhibition on day 1 after the operation (unilateral nephrectomy). P-aminohippuric acid in both media inhibited fluorescein accumulation in renal tubules of sham-operated rats better than in operated rats. It is concluded that either the amount of the carried of transport system for organic acids decreased in membranes of renal tubules of operated rats, or the affinity of carrier to organic acids is aggravated.
D000143:Acids / Q000378:metabolism*; D000222:Adaptation, Physiological; D000818:Animals; D001693:Biological Transport, Active; D005434:Flow Cytometry; D005452:Fluoresceins / Q000378:metabolism; D006626:Hippurates / Q000378:metabolism; D006984:Hypertrophy / Q000378:metabolism; D007668:Kidney / Q000473:pathology*; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D008297:Male; D009392:Nephrectomy; D010042:Ouabain / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000143:Acids; D005452:Fluoresceins; D006626:Hippurates; D010042:Ouabain; C030514:hippuric acid
Rabota sistemy aktivnogo transporta organicheskikh kislot v proksimal'nykh kanal'tsakh pochki krys pri kompensatornoĭ gipertrofii.