[Fractionation of the nucleotides of the acid-soluble liver fraction of rats].
A method for separation of rat liver acid-soluble nucleotides was developed including ion exchange polyethyleneimine cellulose chromatography, followed by rechromatography of the separate fractions on Dowex 1 and Aminex MS resins. It is simple, reproducible and does not require expensive reagents and devices. The sensitivity of the method in respect to orotate is 100 pM in a sample. Data on the content of liver basic 5'-ribonucleotides and their derivatives were obtained by the proposed method.
D000818:Animals; D000837:Anion Exchange Resins; D002458:Cell Fractionation; D002852:Chromatography, Ion Exchange / Q000379:methods; D007475:Ion Exchange Resins; D008099:Liver / Q000032:analysis*; D008722:Methods; D009711:Nucleotides / Q000302:isolation & purification*; D051381:Rats; D012117:Resins, Synthetic; D012995:Solubility
D000837:Anion Exchange Resins; D007475:Ion Exchange Resins; D009711:Nucleotides; D012117:Resins, Synthetic; C027972:Dowex
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