Metabolism of xenobiotics by rat-liver mitochondria: uptake of N,N-dimethyl[14C]amphetamine by rat-liver mitochondria.
Uptake of N,N-dimethyl[14C]amphetamine by freshly isolated rat-liver mitochondria was analysed using a modified Klingenberg's centrifugal layer filtration technique. Influx and efflux of the substrate occurred rapidly at room temperature. Membrane transport, probably an active process, was strongly inhibited at 0 degrees C.
D000818:Animals; D001711:Biotransformation; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D008694:Methamphetamine / Q000031:analogs & derivatives* / Q000378:metabolism; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism; D008930:Mitochondria, Liver / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D013696:Temperature
D008694:Methamphetamine; C003714:dimethylamphetamine