Surface phenotype of Japanese adult T-cell leukemia cells characterized by monoclonal antibodies.
We studies the surface phenotype and the functional activities of leukemic cells from three patients with Japanese adult T-cell leukemic (ATL) using the panel of OK and anti-Tac monoclonal antibodies, which react with differentiation antigens and define functionally distinct T-cell subsets or activated and terminally differentiated T cells. The phenotype of ATL cells were determined to be OKT1+T3+T4+T10+T5-T8-Okla1-, although cells from two patients suppressed pokeweed mitogen (PWM) induced normal B-cell differentiation, and cells from all patients lacked helper activity in this system. In addition, after cultivation with PWM, ATL cells from all patients were reactive with anti-Tac monoclonal antibody, and cells from one patient were reactive with OKlal. These findings suggest that ATL cells arise from peripheral mature T-cell subsets and also suggest that the transition of surface phenotype of ATL cells to functionally mature and activated T cells occurs in culture.
D000328:Adult; D000906:Antibodies; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D006801:Humans; D007564:Japan; D007938:Leukemia / Q000276:immunology*; D010641:Phenotype; D011043:Pokeweed Mitogens / Q000494:pharmacology; D011947:Receptors, Antigen, B-Cell; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000906:Antibodies; D000911:Antibodies, Monoclonal; D011043:Pokeweed Mitogens; D011947:Receptors, Antigen, B-Cell