Serum alkaline phosphatase isoenzymes: the fast liver fraction in the diagnosis of hepatobiliary disease with or without cholestasis.
Serum alkaline phosphatase (ALP EC 3.13.1) isoenzyme patterns were studied in 110 patients with hepatobiliary disease in order to evaluate whether the appearance of the fast liver fraction, absent in normal subjects, could be a marker of cholestatic mechanism. This possibility was studied even when the ALP values are borderline or within normal limits. In conclusion it was found that the fast liver fraction, absent in normal subjects, can satisfactorily discriminate between cholestatic and non-cholestatic disease. Statistical analysis has shown a sensitivity of 98%, a specificity of 92%, a predictable positive value of 90%, a predictable negative value of 98% and a validity of 95%. False positive are 10% and false negative 2%; chi 2 test was 45.008, p less than 0.001. The results show that the presence of this fraction, besides being highly specific, is also an early marker for cholestasis.
D000469:Alkaline Phosphatase / Q000097:blood*; D001651:Cholestasis, Extrahepatic / Q000175:diagnosis*; D004796:Clinical Enzyme Tests; D006521:Hepatitis, Chronic / Q000175:diagnosis; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D008104:Liver Cirrhosis, Alcoholic / Q000175:diagnosis; D008105:Liver Cirrhosis, Biliary / Q000175:diagnosis; D008107:Liver Diseases / Q000175:diagnosis*; D008113:Liver Neoplasms / Q000556:secondary
D007527:Isoenzymes; D000469:Alkaline Phosphatase