[Changes in the electrical and visco-elastic properties of bilayer lipid membranes during interaction with proteins and lipoproteins].
A method for simultaneous registration of planar bilayer lipid membrane (BLM) DC conductance G, capacitance C, surface potential difference delta phi and transversal elasticity module E is developed. C, delta phi and E are proportional to the amplitude of the first, second and third harmonics of capacitance current respectively. A comparative study of the interaction of BLM with very low density lipoproteins (VLDL), influenza virus matrix protein (M-protein) and yeast invertase was carried out. The kinetics of delta phi, E and G changes at different concentrations of VLDL, and dependence of delta phi and G on M-protein and invertase concentration was investigated. It is shown for VLDL invertase and M-protein that the changes in delta phi and E occur before the change in G. The method used permits to study peculiarities of individual stages of interaction between charge particles, supramolecular structures and lipid membranes.
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004553:Electric Conductivity; D008051:Lipid Bilayers; D008074:Lipoproteins; D008564:Membrane Potentials; D011506:Proteins; D014783:Viscosity
D008051:Lipid Bilayers; D008074:Lipoproteins; D011506:Proteins
Izmenenie élektricheskikh i viazkouprugikh svoĭstv bisloĭnykh lipidnykh membran pri vzaimodeĭstvii s belkami i lipoproteidami.