[Isolation of plasma membranes from murine peritoneal macrophages and production of a heterologous immune serum].
Plasma membranes of murine peritoneal macrophages were obtained after light pounding of the cells by differential centrifugation. Subcellular fractions were monitored by specific enzymatic assays and by electron microscopy. Injection of plasma membranes into the rabbit produced an anti-serum containing specific antibodies of macrophage membrane antigen detected by indirect immunofluorescence method.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002458:Cell Fractionation; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D008264:Macrophages / Q000276:immunology / Q000648:ultrastructure*; D051379:Mice; D008854:Microscopy, Electron; D014461:Ultracentrifugation / Q000379:methods
D000954:Antigens, Surface; D007106:Immune Sera
Isolement de membranes plasmiques de macrophages péritonéaux de Souris et production d'un immunsérum hétérologue.