A fluorometric method for the specific determination of serum arginine with 2,3-naphthalenedicarbaldehyde.
A specific and simple fluorometric method for the determination of serum arginine was developed which consists of (1) deproteinization of serum sample with 10% perchloric acid, (2) blocking thiol compounds with N-ethylmaleimide, (3) fluorescence reaction with 2,3-naphthalenedicarbaldehyde in 0.2 M borate buffer, pH 9.3, in the presence of beta-cyclodextrin (25 min at 20 degrees C, excitation wavelength: 462 nm, emission wavelength: 520 nm). Since the fluorescence reaction is highly specific for arginine, the method requires no other separation step. Linearity, recovery, and reproducibility of the method were found to be satisfactory.
D001119:Arginase; D001120:Arginine / Q000097:blood*; D006801:Humans; D006868:Hydrolysis; D008297:Male; D009281:Naphthalenes; D013050:Spectrometry, Fluorescence
D009281:Naphthalenes; C027717:2,3-naphthalenedicarboxaldehyde; D001120:Arginine; D001119:Arginase