[DNA conformation and thermostability in urea aqueous solutions].
It is suggested from the character of the change of circular dichroism spectra that in the presence of urea winding of DNA double helix takes place within the bounds of B-family of forms. It is shown that the realized conformation of DNA differs from the experimentally known forms of DNA belonging to B-family. Urea destabilizes the DNA molecules without connection with helical and melt pairs of DNA nitrous bases. Urea affects the conformational state of DNA by water destruction around DNA, which is accompanied by dehydration of DNA and basic metal ions.
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D012996:Solutions; D013696:Temperature; D013950:Thymus Gland; D014508:Urea
D012996:Solutions; D014508:Urea; D004247:DNA
Konformatsiia i termostabil'nost' DNK v vodnykh rastvorakh mocheviny.