Uncoupler-reversible inhibition of mitochondrial ATPase by metal chelates of bathophenanthroline. I. General features.
(1) Certain metal chelates of 4,7-diphenyl-1,10-phenanthroline (bathophenanthroline, BPh) are potent inhibitors of soluble mitochondrial F1-ATPase. (2) The BPh-metal chelate inhibition of soluble mitochondrial F1-ATPase is relieved by uncouplers of oxidative phosphorylation. (3) The uncouplers appear to interact directly with the inhibitory chelates, forming stoichiometric adducts. (4) A complex between F1 and bPh3Fe2+, containing 3 mol BPh3Fe2+/mol F1, has been isolated. The enzymically inactive F1-BPh3Fe2+ complex binds uncouplers, yielding an enzymically active F1-BPh3Fe2+-uncoupler complex.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D000818:Animals; D002417:Cattle; D002614:Chelating Agents / Q000494:pharmacology*; D007501:Iron / Q000494:pharmacology*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology*; D008928:Mitochondria / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010086:Oxidative Phosphorylation Coupling Factors / Q000494:pharmacology*; D010618:Phenanthrolines / Q000494:pharmacology*; D006180:Proton-Translocating ATPases; D013053:Spectrophotometry; D013329:Structure-Activity Relationship; D013367:Submitochondrial Particles / Q000187:drug effects / Q000378:metabolism*; D014475:Uncoupling Agents / Q000494:pharmacology*
D002614:Chelating Agents; D010086:Oxidative Phosphorylation Coupling Factors; D010618:Phenanthrolines; D014475:Uncoupling Agents; C006686:bathophenanthroline; D007501:Iron; D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases; D008274:Magnesium