Incubation of human lipid transfer protein with plasma from normal-fed and cholesterol-fed rats: effect on the distribution of constituents between lipoprotein fractions.
Samples of rat plasma, in which activity of lecithin-cholesterol acyltransferase was inhibited, were incubated in vitro at 37 degrees C for 6 hr in the presence and absence of a partially purified preparation of human lipid transfer protein (LTP). After the incubation gel filtration chromatography was used to separate plasma lipoproteins into multiple fractions which were individually assayed to obtain a complete profile of all constituents across the whole lipoprotein spectrum. When plasma from both normal and cholesterol-fed rats was incubated in the absence of LTP there were no changes in the distribution of any of the lipoprotein constituents between different fractions. When, however, normal plasma was incubated in the presence of LTP there was an obvious transfer of cholesteryl esters from high density lipoproteins (HDL) to very low density lipoproteins (VLDL) and of triglyceride from VLDL to HDL. The presence of LTP in incubations of plasma from cholesterol-fed rats, by contrast, resulted in only minimal redistributions of constituents.
D000818:Animals; D002352:Carrier Proteins / Q000097:blood*; D002788:Cholesterol Esters / Q000097:blood; D002791:Cholesterol, Dietary / Q000008:administration & dosage*; D008076:Cholesterol, HDL / Q000097:blood; D002850:Chromatography, Gel; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male; D010316:Particle Size; D051381:Rats; D014280:Triglycerides / Q000097:blood
D002352:Carrier Proteins; D002788:Cholesterol Esters; D002791:Cholesterol, Dietary; D008076:Cholesterol, HDL; D008075:Lipoproteins, HDL; D008079:Lipoproteins, VLDL; D014280:Triglycerides; C030363:lipid transfer protein