Changes in quantities of high-mobility-group protein 1 in oviduct cellular fractions after oestrogen stimulation.
Antiserum against chick oviduct high-mobility-group protein 1 (HMG 1) has been induced in the rabbit. With this antiserum, immunobiochemical techniques have been used to probe the quantitative change of HMG 1 in the cellular fractions of chick oviduct before or after oestrogen stimulation. HMG 1 is detectable in the cytosol, microsomal and nuclear fraction of the chick oviduct cell. After administration of oestrogen to young chicks in vivo for 5 days, the quantity of HMG 1 is increased 4-fold in the cytosol, 3.5-fold in the microsomal fraction and 1.6-fold in the nuclear fraction. The finding of large amounts of HMG 1 in cytoplasm of oviduct cell is not likely due to its leakage from the nucleus. We anticipate that HMG 1 is synthesized in the cytoplasm and then transported into the nucleus. The synthesis and transportation of HMG proteins is probably regulated by oestrogen.
D000818:Animals; D002645:Chickens; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D003168:Complement Fixation Tests; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006609:High Mobility Group Proteins; D010057:Oviducts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011863:Radioimmunoassay; D013268:Stimulation, Chemical; D013347:Subcellular Fractions / Q000378:metabolism
D002868:Chromosomal Proteins, Non-Histone; D006609:High Mobility Group Proteins; D004958:Estradiol