Chicken reticulocyte polysomal messenger RNA-protein complex: absence of bound proteins in most of the coding region of beta globin mRNA.
The 15s globin mRNA-protein complex (mRNP) was isolated from chicken reticulocyte polyribosomes dissociated in EDTA. To determine protein binding sites, the mRNP was treated with micrococcal nuclease and the nuclease resistant RNA was mapped to the beta globin gene at the nucleotide level. As far as we can determine there is no bound protein from the Cap site to the poly A addition site of beta globin mRNA in the mRNP except for a short area in the coding region near the translation initiation site.
D000818:Animals; D001665:Binding Sites; D002645:Chickens; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics*; D008836:Micrococcal Nuclease; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012156:Reticulocytes / Q000502:physiology; D012261:Ribonucleoproteins / Q000235:genetics*
D012333:RNA, Messenger; D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein; D005914:Globins; D008836:Micrococcal Nuclease