Effect of separating bull semen into X and Y chromosome-bearing fractions on the sex ratio of resulting embryos.
Seventy-six, day 12 to day 15 bovine embryos, collected from 14 donors which had been inseminated with either X or Y chromosome-bearing spermatozoa fractions of semen separated by a thermal convection counterstreaming sedimentation and forced convection galvanization process, were processed for sexing by chromosomal analysis. Fifty-seven embryos were sexed; 20 from Y chromosome-bearing and 37 from X chromosome-bearing fractions of semen. Statistical analysis of the sexing data indicated that there was no significant difference in the male: female ratio for donors receiving male fractions compared to those receiving female fractions. The Y chromosome-bearing fractions produced a male: female ratio that was indistinguishable from the expected 1:1 ratio. However, the X chromosome-bearing fractions of semen produced a highly significant deviation from the expected 1:1 ratio towards the male.
D000818:Animals; D002417:Cattle / Q000502:physiology*; D004622:Embryo, Mammalian / Q000648:ultrastructure*; D005260:Female; D005865:Gestational Age; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary; D008297:Male; D011247:Pregnancy; D012661:Semen / Q000032:analysis*; D012732:Sex Determination Analysis; D012744:Sex Ratio; D013480:Superovulation; D014960:X Chromosome / Q000032:analysis*; D014998:Y Chromosome / Q000032:analysis*