Clinical prediction of experimental chemotherapy of gynecological tumor xenografts.
Routine transplantations of gynecological malignomas on thymus aplastic nu/nu mice (NMRI) are reported. In the course of five years more than 450 different malignous tumours were successfully xenotransplanted on nude mice subcutaneously. The take-off-rate was different: ranging from fifty percent of mamma carcinomas to almost eighty percent of ovarian carcinomas. For experiments not the take-off-rate is relevant, but growth acceleration, and therefore the number of tumours for experimental therapy shrinks to less than twenty percent of mamma carcinomas and to about fifty percent of ovarian carcinomas. The human origin of the xenotransplants-even in later animal passages-could be determined in all cases by the presence of isoenzymes Es D, LDH 1 and LDH 2. But the growing rate of mitosis indicates a change of cell proliferation of the xenotransplants. Experimental chemotherapy was performed with accepted clinical drugs (Cyclophosphamide, Platin, Adriamycin), different growth retardation was observed dependent on tumour kind: i.e. ovarian carcinomas were stronger retarded than cervical and endometrial cancers. Yet in ovarian carcinomas the individual comparison between effect in xenotransplant and patient is full of problems. The nude mice model is excellent as a preclinical prediction for the effect of newly developed cytostatics or the relevance of different therapeutical approaches.
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D003520:Cyclophosphamide / Q000494:pharmacology; D004950:Esterases / Q000378:metabolism; D005260:Female; D005833:Genital Neoplasms, Female / Q000188:drug therapy* / Q000648:ultrastructure; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D008938:Mitosis; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000188:drug therapy; D010051:Ovarian Neoplasms / Q000188:drug therapy; D013997:Time Factors; D014183:Transplantation, Heterologous
D000970:Antineoplastic Agents; D007527:Isoenzymes; D003520:Cyclophosphamide; D007770:L-Lactate Dehydrogenase; D004950:Esterases