A method for eliminating Rayleigh scattering from fluorescence spectra.
A numerical method is described for the elimination of Rayleigh scattering from protein fluorescence emission spectra. The method is based upon the observation that Rayleigh scattering is symmetrical about a wavelength at or near the wavelength of excitation. It works best when an automated, computer-based approach can be applied to spectra collected on a data-logging device, although manual correction of chart-recorded spectra is also possible.
D002135:Calcium-Binding Proteins / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D006003:Glycogen / Q000032:analysis; D008027:Light; D012542:Scattering, Radiation; D012997:Solvents; D013050:Spectrometry, Fluorescence / Q000379:methods*
D002135:Calcium-Binding Proteins; D012997:Solvents; D006003:Glycogen