Isolation of protein(s) containing chloride ion transport activity from thylakoid membranes.
Extracts of detergent-treated thylakoids, when reconstituted into azolectin/cholesterol/dicetyl phosphate vesicles, stimulate chloride ion efflux as measured with a Cl--sensitive electrode. This stimulation is inhibited by piretanide, an active chloride ion transport inhibitor in fish intestinal epithelia. This stimulation is also abolished by pretreatment of extracted proteins with trypsin. Antiserum raised to efflux-active proteins inhibits a cation-driven Cl- influx in nonenergized thylakoids, as measured by a flow dialysis technique.
D001693:Biological Transport, Active / Q000187:drug effects; D002712:Chlorides / Q000378:metabolism*; D002736:Chloroplasts / Q000032:analysis* / Q000378:metabolism; D003956:Dialysis / Q000379:methods; D005374:Filtration; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D007425:Intracellular Membranes / Q000032:analysis; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism; D008567:Membranes, Artificial; D010940:Plant Proteins / Q000302:isolation & purification* / Q000378:metabolism; D013449:Sulfonamides / Q000494:pharmacology; D014634:Valinomycin / Q000494:pharmacology
D002712:Chlorides; D007106:Immune Sera; D008565:Membrane Proteins; D008567:Membranes, Artificial; D010940:Plant Proteins; D013449:Sulfonamides; D014634:Valinomycin; C015451:piretanide