The two-dimensional polypeptide profile of terminally non-dividing human diploid cells.
We have used high resolution two-dimensional polyacrylamide gel electrophoresis (PAGE) to compare the polypeptides synthesized by normal human diploid fibroblasts cells which have significant proliferative capacity with those of cells which are terminally non-dividing. Normal cells that are terminally non-dividing (i.e., have no remaining proliferative potential) synthesize two polypeptides that are not detected in young cells which are either actively proliferating or growth arrested by incubation in medium containing a low concentration of serum (0.3%) for 72 h. Continued maintenance of young cells in the growth-arrested state ultimately leads to the detectable synthesis of one of the polypeptides synthesized by terminally non-dividing cells. Some preliminary biochemical properties of these two polypeptides are examined.
D002453:Cell Cycle; D002460:Cell Line; D002999:Clone Cells; D004171:Diploidy; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007930:Leucine / Q000378:metabolism; D008168:Lung / Q000166:cytology; D010455:Peptides / Q000032:analysis*; D013462:Sulfur Radioisotopes; D014316:Tritium
D010455:Peptides; D013462:Sulfur Radioisotopes; D014316:Tritium; D007930:Leucine