[Translation of mRNA extracted from human placenta in a wheat germ cell free system].
The mRNA were isolated from total RNA extracted from placenta by affinity chromatography on poly U Sepharose 4 B and were tested with wheat germ cell free system. The neosynthesized hPL is isolated by specific immunoprecipitation using 2 antibodies from the CEA (hPLK1 and hPLK3). It represented 7% of the total radioactive synthesized proteins. Two components were separated by gel electrophoresis. One is the natives hormone while the other which is the major component, migrated with a molecular weight of 25 000. These results are in accordance with a functionally cell free system which could be used study the non histone protein's role in the hPL synthesis.
D002474:Cell-Free System; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D007700:Kinetics; D010920:Placenta / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D011247:Pregnancy; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D014908:Triticum / Q000378:metabolism
D012333:RNA, Messenger
Traduction en système acellulaire de germe de blé des mRNA extraits de placenta humain.