Analysis of asparagine-linked oligosaccharides from plasma membranes of rat normal liver and ascites hepatoma cells.
Isolated plasma membranes from rat liver and ascites hepatoma cells were shown by SDS-polyacrylamide gel electrophoresis and concanavalin A reactivity to contain a variety of glycoproteins having asparagine-linked oligosaccharides. Membrane oligosaccharides were released by almond glycopeptidase digestion, and the pyridylamino derivatives were analyzed by high-performance liquid chromatography. Forty-four percent of the total carbohydrates in the original membranes were released and suggested to be of the complex type. Hepatoma membranes showed different oligosaccharide patterns from normal.
D000818:Animals; D001216:Asparagine; D002462:Cell Membrane / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D006020:Glycopeptides / Q000032:analysis; D006023:Glycoproteins / Q000302:isolation & purification*; D008099:Liver / Q000032:analysis*; D008114:Liver Neoplasms, Experimental / Q000032:analysis*; D008565:Membrane Proteins / Q000302:isolation & purification*; D009844:Oligosaccharides / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D006020:Glycopeptides; D006023:Glycoproteins; D008565:Membrane Proteins; D009844:Oligosaccharides; D001216:Asparagine