Enzymatic deoxyglucosylation of ceramides by microsomes of BHK-21 cells. The effect of deoxyglucose treatment and herpesvirus infection.
The microsomal fractions of cultured hamster fibroblasts (BHK-21 cells) catalyze the incorporation of glucose from UDPglucose or of deoxyglucose from UDPdeoxyglucose into a reaction mixture with liposomes consisting of ceramide and phosphatidylcholine. The microsomal fractions also catalyze the transfer of glucose from UDPglucose to endogenous acceptors. The specific activity of ceramide deoxyglucoside or ceramide glucoside formation was significantly higher when microsomal preparations obtained from deoxyglucose-treated or herpesvirus-infected BHK-21 cells were used as the glucosyltransferase source. Deoxyglucose was incorporated from UDPdeoxyglucose into hydroxy- and nonhydroxy-fatty acid-containing ceramides at approximately the same rate. Competitive inhibition of deoxyglucosylation of ceramides by UDPglucose suggests that both reactions were catalyzed by the same enzyme, viz. UDPglucose:ceramide glucosyltransferase. This inhibition of glycosphingolipid synthesis may account, in part, for the inhibitory effect of deoxyglucose on lipid-containing viruses.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D002518:Ceramides / Q000378:metabolism*; D006224:Cricetinae; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D005964:Glucosyltransferases / Q000378:metabolism*; D006564:Herpesviridae / Q000235:genetics*; D007668:Kidney; D007700:Kinetics; D008081:Liposomes; D008861:Microsomes / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism
D002518:Ceramides; D003837:Deoxy Sugars; D008081:Liposomes; D010713:Phosphatidylcholines; D003847:Deoxyglucose; D005964:Glucosyltransferases; C023246:ceramide glucosyltransferase