Isoelectric focusing in agarose: phosphoglucomutase (PGM locus 1) typing.
The subphenotypes of phosphoglucomutase (PGM) was successfully typed by isoelectric focusing with a charge-balanced agarose. The resolution was as high as that obtained with polyacrylamide, with none of the drawbacks associated with the use of polyacrylamide. Exceptionally straight iso-pH lines were obtained, making interpretation of the results more reliable. Gel preparation was simple and rapid, and the time for analysis was reduced. A way of preserving the resultant zymogram is described.
D001801:Blood Stains; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D010733:Phosphoglucomutase / Q000235:genetics*; D012685:Sepharose
D012685:Sepharose; D010733:Phosphoglucomutase