Effect of cyclosporin A on the generation of cytotoxic T lymphocytes in mouse mixed lymphocyte culture.
Cyclosporin A inhibited at equal concentrations both the proliferative response and the generation of cytotoxic T lymphocytes in one-way mouse spleen cell mixed lymphocyte culture. The 50% inhibitory concentration was in all experiments 10(-2) to 10(-1) microgram/ml. The inhibition was directly proportional to how early the drug was added to the culture: a complete inhibition of both responses was obtained if the drug was added on day 3, and a partial inhibition if added on day 4 of culture. Cytological analysis of the cultured cells demonstrated that resting lymphocytes were not damaged at 100-1000-fold concentrations of the drug giving complete inhibition of the blastogenic response. The results suggest that cyclosporin A is most effective if present throughout the induction phase of the immune response.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003524:Cyclosporins; D003602:Cytotoxicity, Immunologic; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D010456:Peptides, Cyclic / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D013997:Time Factors
D003524:Cyclosporins; D010456:Peptides, Cyclic