Aspartate aminotransferase in Leishmania is a broad-spectrum transaminase.
The substrate specificity of aspartate aminotransferase (ASAT, E.C. 2.6.1.1.) from Leishmania was examined following observations of artefacts on gels stained for alanine aminotransferase (ALAT, E.C. 2.6.1.2.) after thin-layer starch-gel electrophoresis. Leishmanial ASAT acted on L-aspartate, L-alanine, L-tryptophan and L-tyrosine. Interpretation of ALAT zymograms must thus take into account the presence of interfering ASAT bands, and the need is emphasized for rigorous controls in isoenzyme electrophoresis.
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D004592:Electrophoresis, Starch Gel; D007891:Leishmania / Q000201:enzymology*; D013379:Substrate Specificity; D000637:Transaminases / Q000378:metabolism
D000637:Transaminases; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase