Studies on the characterization of the linkage-region between polysaccharide chain and core protein in bovine corneal proteokeratan sulfate.
1) A new method of enrichment of the linkage-region in corneal proteokeratan sulfate is described, which consists of desulfation of peptidokeratan sulfate, followed by chromatography on Con A-Sepharose 4B and enzymatic degradation with beta-D galactosidase and beta-N-acetyl-D-glucosaminidase. 2) After permethylation, hydrolysis, reduction with sodium borohydrid and acetylation gas chromatography/mass spectrometry analyses were performed. The followings products could be detected as their peracetates: 2,3,4-tri-O-methylfucitol; 2,3,4,6-tetra-O-methylmannitol; 3,4,6-tri-O-methylmannitol; 2,4-di-O-methylmannitol; 2,3,4,6-tetra-O-methylgalactitol; 2,4,6-tri-O-methylgalactitol; 2,4-di-O-methylgalactitol. 3) The results point to the presence of a branched linkage region in the proteokeratan sulfate molecule with one mannose as the branching point and two mannose residues as the starting point of two disaccharide chains.
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D011508:Chondroitin Sulfate Proteoglycans; D003315:Cornea / Q000032:analysis*; D005136:Eye Proteins / Q000032:analysis*; D006025:Glycosaminoglycans; D007632:Keratan Sulfate; D000071234:Lumican; D008745:Methylation; D011134:Polysaccharides / Q000032:analysis*; D011509:Proteoglycans
D011508:Chondroitin Sulfate Proteoglycans; D005136:Eye Proteins; D006025:Glycosaminoglycans; D000071234:Lumican; D011134:Polysaccharides; D011509:Proteoglycans; D007632:Keratan Sulfate