Dermal oncogenicity studies on ethylenediamine in male C3H mice.
The dermal oncogenic potential of ethylenediamine (EDA) was assessed by applying 25 microliter of a 1% solution in deionized water to the skin of 50 male C3H/HeJ mice. This was the highest concentration not producing irritation or weight changes in a preliminary 2-week study. Two EDA samples (Nos. 1 and 2) from different production sources were tested. Applications were made thrice weekly until the death of the animals. A negative control group received deionized water only. This group and the EDA-treated groups were individually housed. A fourth group of 40 mice, housed 5 per cage, received 0.1% 3-methylcholanthrene (MC) in acetone as a positive control substance. A fifth group of 40 mice, housed 5 per cage, also received deionized water to determine the effect of group housing on survival. No skin tumors were observed in the EDA-treated groups. In the positive control group, however, 39 animals (98%) had skin tumors including 37 (92%) with confirmed squamous cell carcinomas. Eleven mice (22%) which received EDA No. 1 had dermal fibrosis indicative of probable skin irritation in this group; there was no such lesion in the controls. The mean survival times were 639, 626, and 598 days for the EDA No. 1, water control, and EDA No. 2 groups, respectively. The survival time of the EDA No. 2 group was significantly reduced compared to the individually housed water controls by one of two statistical tests. Irrespective of this difference, the study is considered to be a valid assessment of the oncogenic potential of EDA No. 2 because the magnitude of the difference in mean survival time was small.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D005029:Ethylenediamines / Q000633:toxicity*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D012878:Skin Neoplasms / Q000139:chemically induced*; D013997:Time Factors
D005029:Ethylenediamines; C031234:ethylenediamine