Quantitation of the delivery of liposome contents into plant protoplasts.
A procedure for the quantitation of the delivery of liposome contents into Catharanthus roseus protoplasts has been developed. The method is based on the uptake of liposome encapsulated methylumbelliferyl beta-D-glucoside and its enzymatic hydrolysis to yield fluorescent methylumbelliferone. Since the free glucoside is not taken up by the protoplasts to a significant extent, the delivery of material in the nanomole range can be measured with ease.
D001692:Biological Transport; D002463:Cell Membrane Permeability; D005959:Glucosidases / Q000378:metabolism; D005960:Glucosides / Q000378:metabolism; D006868:Hydrolysis; D006923:Hymecromone / Q000031:analogs & derivatives / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism*; D010944:Plants / Q000201:enzymology / Q000378:metabolism*; D011523:Protoplasts / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity
D005960:Glucosides; D008081:Liposomes; C014110:4-methylumbelliferyl glucoside; D006923:Hymecromone; D005959:Glucosidases