[Manual method of assaying plasma hemoglobin by tetramethylbenzidine and deferoxamine].
We describe a manual method to determine very low concentrations of hemoglobin in plasma (0,25 to 37,3 mumol/l). We use peroxidase properties of hemoglobin which decomposes hydrogen peroxide releasing oxygen. This released oxygen will oxidize tetramethylbenzidine. The original feature of the proposed method lies in the stabilization of the obtained colour by a complexing agent, the deferoxamine. The technique is quick, reliable, cheap and does not need specialized equipment. It is possible to use the method for urines.
D001560:Benzidines; D003676:Deferoxamine; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D013053:Spectrophotometry
D001560:Benzidines; D006454:Hemoglobins; C021758:3,3',5,5'-tetramethylbenzidine; D003676:Deferoxamine
Méthode manuelle de dosage de l'hémoglobine plasmatique par la tétraméthylbenzidine et la déferoxamine.