Synthesis of early vaccinia-virus-specific enzymes under conditions of immediate early gene expression.
Cycloheximide reversal experiments in chick embryo fibroblasts and mouse L-929 cells indicate that the poxvirus-induced enzymes DNA polymerase and 'alkaline' DNase are immediate early gene products of the virus. In contrast to the vaccinia-WR-coded enzyme under conditions of immediate early gene expression the cowpox-virus-induced DNA polymerase is made only in very small amounts. The studies are consistent with the notion that all poxvirus-specific early proteins may be immediate early viral gene products.
D000818:Animals; D002642:Chick Embryo; D004259:DNA-Directed DNA Polymerase / Q000096:biosynthesis*; D003851:Deoxyribonucleases / Q000096:biosynthesis*; D004790:Enzyme Induction; D005347:Fibroblasts; D005786:Gene Expression Regulation; D006863:Hydrogen-Ion Concentration; D007739:L Cells; D051379:Mice; D013997:Time Factors; D014616:Vaccinia virus / Q000201:enzymology* / Q000235:genetics; D014776:Virus Cultivation
D004259:DNA-Directed DNA Polymerase; D003851:Deoxyribonucleases