Kinetics of cytoplasmic aspartate aminotransferase from three genotypes of the deer mouse (Peromyscus maniculatus).
Three genotypic forms of the cytoplasmic enzyme, aspartate aminotransferase from the deer mouse (Peromyscus maniculatus) were each analyzed kinetically after partial purification. Kmapp and Vmax for aspartate decreased in value as temperature changed from 37.9 to 15 degrees C for all three forms. For the AA genotype, the binding enthalpies were highest at 37.9 degrees C and lowest at 25 degrees C, while for the A'A' form, they were lowest at 37.9 degrees and highest at 25 degrees C. Results for the heterozygote were generally intermediate reflecting the properties of both subunits. Inhibition by glyceraldehyde-3-phosphate was of a non-competitive type for all three genotypes.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D003593:Cytoplasm / Q000201:enzymology*; D005838:Genotype; D005986:Glyceraldehyde 3-Phosphate / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010542:Peromyscus / Q000235:genetics* / Q000378:metabolism; D013696:Temperature
D005986:Glyceraldehyde 3-Phosphate; D001219:Aspartate Aminotransferases