The proton gradient across the vacuo-lysosomal membrane of lutoids from the latex of Hevea brasiliensis. I. Further evidence for a proton-translocating ATPase on the vacuo-lysosomal membrane of intact lutoids.
Lutoids (vacuo-lysosomal particles) were isolated from the latex of Hevea brasiliensis. Using flow dialysis with 14C-methylamine uptake as a delta pH probe and 86Rb rubidium + valinomycin distribution for estimations of transmembrane electrical potential, intact lutoids exhibited a delta pH of 1 unit (interior more acid) and a delta psi of -70 mV (interior negative), when suspended in an isotonic medium at physiological concentrations of potassium (30 mM) and pH 7.0, in the absence of ATP. In most cases, the Donnan potential was shown to fully account for delta pH in nonenergized lutoids. The addition of MG-ATP (5 mM) resulted in a marked acidification of the lutoidic internal space (0.7 to 1 pH unit) depending on the composition of the medium, and in a membrane depolarization by 60 mV (interior becoming less negative). The resulting electrochemical potential of protons (delta approximately microH) increased by a hundred millivolts when lutoids were energized by ATP. These data strongly support an inward electrogenic proton translocating function for the ATPase of the vacuo-lysosomal membrane of lutoids. Results are discussed in terms of the in vivo maintenance of large "lutoids/cytoplasm" proton gradients, and of the rôle of these vacuo-lysosomes in the homeostasis of the cytoplasmic metabolism.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D001692:Biological Transport; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000201:enzymology*; D007700:Kinetics; D007840:Latex; D008247:Lysosomes / Q000201:enzymology*; D009940:Organoids / Q000201:enzymology*; D010944:Plants / Q000201:enzymology; D006180:Proton-Translocating ATPases; D014617:Vacuoles / Q000201:enzymology*
D007840:Latex; D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases