Survival of rabbit embryos after rapid freezing and thawing.
Frozen storage of rabbit embryos at the 16-cell stage in 2.0 M dimethylsulfoxide (DMSO) in phosphate-buffered saline (PBS) was achieved by a 2-step procedure. After storage for 10 days at -196 degrees C they were revived by rapidly thawing at 500 degrees C/min. On transfer of these embryos to pseudopregnant foster mothers, 50% survived to term. The difference in in vivo survival between frozen-thawed and frozen-thawed-cultured embryos was not significant.
D000818:Animals; D046508:Culture Techniques; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000502:physiology*; D005615:Freezing; D006358:Hot Temperature; D011817:Rabbits; D014021:Tissue Preservation