Regulation of in vitro primary anti-DNP antibody production by functional subsets of T lymphocytes in man.
A primary in vitro antibody response to DNP-KLH by peripheral blood lymphocytes in culture was developed. Optimal anti-DNP antibody production, as measured by solid phase radioimmunoassay occurred at a cell density of 1 X 10(6), and an antigen concentration of 5 to 10 micrograms per culture when PBL was incubated with the antigen for 5 days and then cultured in the absence of DNP-KLH for 4 additional days. The antibody produced was shown to be primarily of the IgM isotype and was specific for DNP. In the absence of T lymphocytes, no antibody was generated. The regulatory effects of T4+ and T5+/T8+ subsets on antigen-specific antibody production were determined and it was found that T4+ subset alone provided help for anti-DNP antibody production. In contrast, the T8+ subset did not provide help and more importantly, could suppress anti-DNP antibody production in the presence of T4+ inducer T cells. This in vitro antibody forming system should be of considerable use in the analysis of the cellular requirements for antibody production and genetic control of the immune response in man.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D002417:Cattle; D002469:Cell Separation; D004136:Dinitrobenzenes / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D006433:Hemocyanins / Q000276:immunology; D006801:Humans; D009578:Nitrobenzenes / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D004136:Dinitrobenzenes; D009578:Nitrobenzenes; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D006433:Hemocyanins