Studies on heterogeneous lipopolysaccharide fractions of Vibrio cholerae 569B.
By SDS-PAGE or gel filtration on Sephadex G-25, lipopolysaccharide (LPS) isolated from Vibrio cholerae 569B (Inaba) can be separated into two distinct fractions, one corresponding to smooth LPS and the other to rough LPS. Pulse-labelling of LPS with [14C]glucose showed that the rough form is synthesized first followed by the biosynthesis of the smooth form. A preferential release of the smooth LPS of V. cholerae 569B was also detected during normal growth of cells.
D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000378:metabolism; D008070:Lipopolysaccharides / Q000032:analysis* / Q000096:biosynthesis; D014734:Vibrio cholerae / Q000032:analysis* / Q000378:metabolism
D008070:Lipopolysaccharides; D005947:Glucose