RNA-containing nuclear binding sites for glucocorticoid-receptor complexes.
RNase A treatment of HeLa cell nuclei causes a time- and concentration-dependent release of dexamethasone-receptor complexes. If nuclei are incubated in the absence of enzyme, only 60% of RNase-releasable complexes can be detected. Sucrose density gradient analysis of nuclear extracts shows that receptor complexes released by RNase treatment sediment at 3.6 S, whereas complexes obtained from untreated nuclei sediment between 7 and 3.6 S. Our results show that a fraction of dexamethasone-receptor complexes retained by HeLa cell nuclei is located in binding sites involving RNA.
D002467:Cell Nucleus / Q000378:metabolism*; D003907:Dexamethasone / Q000378:metabolism; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D012334:RNA, Neoplasm / Q000302:isolation & purification* / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000302:isolation & purification* / Q000378:metabolism; D011987:Receptors, Steroid / Q000302:isolation & purification*; D012259:Ribonuclease, Pancreatic / Q000378:metabolism
D012334:RNA, Neoplasm; D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; C028697:dexamethasone receptor; D003907:Dexamethasone; D012259:Ribonuclease, Pancreatic