Adrenal function and the action of propranolol in surgical stress-mediated insulin resistance of glucose utilization by peripheral tissues in vivo. Inhibition of glucose accumulation in skeletal muscle.
The effect of acute adrenalectomy and the action of propranolol on insulin-stimulated glucose utilization was investigated in an in vivo model of peripheral tissues of the rabbit after evisceration and nephrectomy. The lactate/pyruvate ratios in muscle and blood and the content of energy-rich phosphate compounds in muscle were not altered during the experiments. The results obtained in animals with and without intact adrenals were compared. The insulin-mediated glucose utilization was inhibited by 34% in animals with intact adrenals. In the semimembranosus muscle of adrenalectomized rabbits the parameters of glucose metabolism corresponded to those in the 'resting state'. In the muscle of animals with intact adrenals the content of glycogen was decreased and that of glycolytic intermediates was elevated; free glucose was accumulated in the muscle cell. Alterations of the glucose content in skeletal muscle were inversely correlated with the glucose utilization of peripheral tissues. DL-Propranolol (0.05 mg/kg) administered before surgery i.v. to rabbits with intact adrenals not only prevented glycogenolysis and accumulation of glycolytic intermediates but also the stress-mediated glucose accumulation in skeletal muscle. Propranolol abolished the insulin resistance of glucose utilization of peripheral tissues mediated by the adrenals after surgical preparation of the rabbits.
D000311:Adrenal Glands / Q000502:physiology*; D000315:Adrenalectomy; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism*; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000378:metabolism; D007333:Insulin Resistance / Q000187:drug effects*; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D011433:Propranolol / Q000494:pharmacology*; D011817:Rabbits; D013312:Stress, Physiological / Q000503:physiopathology*; D013997:Time Factors
D001786:Blood Glucose; D005958:Glucosephosphates; D007773:Lactates; D019344:Lactic Acid; D019298:Glucose-6-Phosphate; D011433:Propranolol; D005947:Glucose