Predictive animal testing for photocontact allergenicity.
The purpose of this investigation was to establish a standardized animal model to predict the photoallergenic potential of new chemical compounds. In a first series of experiments the main factors influencing the induction of photoallergenicity were evaluated (induction concentration, pH of the test solution, pretreatment of the irradiation site with sodium lauryl sulphate, additional use of adjuvant injections and routes of administration). Osram Ultravitalux lamps were utilized for these studies. In a second series of experiments a Xenon 6000 W radiation source was used. Positive results were obtained with well known photosensitizers, as, for instance, the halogenated salicylanilides, as well as with so-called weak photoallergens (e.g. bithionol, sulphanilamide) by stimulating the animals' immune system with Freund's complete adjuvant. The photoallergenic compounds were at the same time tested for their contact allergenic potential in the optimization test. A standard procedure for the predictive photoallergenicity assay in guinea-pigs is proposed.
D000276:Adjuvants, Immunologic; D000818:Animals; D003877:Dermatitis, Contact / Q000139:chemically induced; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D003875:Drug Eruptions / Q000209:etiology*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005260:Female; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D008297:Male; D010787:Photosensitivity Disorders / Q000139:chemically induced*; D012882:Skin Tests; D014466:Ultraviolet Rays
D000276:Adjuvants, Immunologic