The electrophoretic mobilities and activities of eleven enzymes of bloodstream and culture forms of Trypanosoma brucei compared.
Eleven soluble enzymes in the supernatant of bloodstream Trypanosoma brucei were compared for electrophoretic mobility and activity with those of T. brucei cultures grown in 3 different media. All bands of each enzyme found in the bloodstream form were also present in the cultured material, but extra bands of malate dehydrogenase (MDH) (EC 1.1.1.37), aspartate aminotransferase (ASAT) (EC 2.6.1.1), and in 2 to 6 cultures of isocitrate dehydrogenase (ICD) (EC 1.1.1.42) were present in culture forms but not in bloodstream forms. An interfering enzyme, peculiar to cultured T. brucei, which reacted with 2-oxoglutarate and possibly a trace amount of ammonium ions, ran with the fast-moving ASAT bands. Threonine dehydrogenase activity, high in cultured trypanosomes irrespective of the medium used but low in bloodstream trypanosomes, was markedly lower in Trypanosoma evansi and a much passaged T. brucei 8/18. Glucosephosphate isomerase activity on the other hand was high in bloodstream and low in cultured trypanosomes. Glutamate dehydrogenase activity was too low to record reliably in bloodstream trypanosomes, but could be clearly detected in cultured forms. As the differences point to some changes in gene expression between the two forms, culture material is likely to replace trypanosomes from living animals for electrophoretic characterization only when considerable comparative work has been done.
D000410:Alanine Transaminase / Q000302:isolation & purification; D000429:Alcohol Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000302:isolation & purification; D002162:Camelus; D004586:Electrophoresis; D005956:Glucose-6-Phosphate Isomerase / Q000302:isolation & purification*; D005969:Glutamate Dehydrogenase / Q000302:isolation & purification; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000302:isolation & purification; D006153:Guanosine Diphosphate / Q000302:isolation & purification; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000302:isolation & purification; D007527:Isoenzymes / Q000302:isolation & purification*; D008291:Malate Dehydrogenase / Q000302:isolation & purification; D051379:Mice; D010088:Oxidoreductases / Q000302:isolation & purification*; D010733:Phosphoglucomutase / Q000302:isolation & purification*; D051381:Rats; D013552:Swine; D000637:Transaminases / Q000302:isolation & purification*; D014345:Trypanosoma / Q000201:enzymology*; D014370:Tsetse Flies
D007527:Isoenzymes; D006153:Guanosine Diphosphate; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; C023159:L-threonine 3-dehydrogenase; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005969:Glutamate Dehydrogenase; D000637:Transaminases; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase