Capillary gas chromatographic/mass spectrometric analysis of abnormal metabolites in hypoglycin-treated rat urine.
Numerous abnormal metabolites were identified in large amounts in the urine of hypoglycin-treated rats using capillary gas chromatography/mass spectrometry-computer analysis. These metabolites are not detectable in significant amounts in normal rats' urine. Ten of them have not been previously associated with hypoglycin administration: these are several hydroxy compounds, including those from the valine and isoleucine pathways, 2-oxo-adipic acid, n-butyrylglycine and isovaleryl glucuronide. These results indicate that the pathways of isoleucine and valine metabolism are inhibited at their respective acyl-CoA dehydrogenation steps, as is the case for fatty acid, leucine and lysine metabolism, as previously shown. The mass spectra of the trimethylsilyl derivatives of cis, cis-4,7-decadiene-1,10-dioic, cis-4-decene-1,10-dioic, cis-4-octene-1,8-dioic acids, and (methylenecyclopropyl)acetylglycine, which were previously identified using nuclear magnetic resonance and oxidative cleavage or acid hydrolysis, are presented for the first time.
D000272:Adipates / Q000652:urine; D000818:Animals; D003521:Cyclopropanes / Q000494:pharmacology*; D003998:Dicarboxylic Acids / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D005965:Glucuronates / Q000652:urine; D020723:Glucuronic Acid; D005966:Glucuronidase; D005998:Glycine / Q000031:analogs & derivatives / Q000652:urine; D006880:Hydroxy Acids / Q000652:urine*; D007005:Hypoglycins / Q000494:pharmacology*; D007532:Isoleucine / Q000037:antagonists & inhibitors*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014631:Valerates / Q000652:urine; D014633:Valine / Q000037:antagonists & inhibitors*
D000272:Adipates; D003521:Cyclopropanes; D003998:Dicarboxylic Acids; D005965:Glucuronates; D006880:Hydroxy Acids; D007005:Hypoglycins; D014631:Valerates; D007532:Isoleucine; C041757:hypoglycin; C043029:N-butyrylglycine; C039947:isovalerylglucuronide; D020723:Glucuronic Acid; C001918:alpha-ketoadipic acid; D005966:Glucuronidase; D014633:Valine; D005998:Glycine