Predominance of beta-structure in solubilized zona pellucida from porcine ova.
The isolated zona pellucida from porcine ova was effectively solubilized in water at 60 degrees C within one hour. The circular dichroic spectra of zona in water with and without dithiothreitol showed the beta-form. Although sodium dodecyl sulfate partially induced helical structure, the beta-form was considerably retained. These results indicate that the zona glycoproteins have a structure-forming potential for the beta-structure and the hydrogen bonds of the beta-structure stabilize the supramolecular complex of the zona pellucida. The beta-form was also detected in zona solubilized by tryptic digestion. Porcine acrosin, however, did not solubilize the zona.
D000818:Animals; D002458:Cell Fractionation; D002942:Circular Dichroism; D004229:Dithiothreitol / Q000494:pharmacology; D005260:Female; D010063:Ovum / Q000648:ultrastructure*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D012995:Solubility; D013552:Swine; D015044:Zona Pellucida / Q000187:drug effects / Q000648:ultrastructure*
D012967:Sodium Dodecyl Sulfate; D004229:Dithiothreitol