[Electron microscopy study of deoxyribonuclease penetration into chick embryo fibroblasts and the effect of the enzyme on the cells].
Peroxidase labeled DNase (0.25 g/ml) penetrated into the chick fibroblast culture cells by means of pinocytosis to be localized in pinocytotic vacuoles. Within a 24 hour-incubation of the cell culture with DNase a relative volume of vacuoles in cells increased from 4.6 to 19.5 per cent, the majority of pinocytic vacuoles being filled with labeled DNase. The incubation of cells with non-labeled DNase (24 hours) did not damage DNA-containing cell structures. The data obtained permit to explain the selective effect of the enzyme on the viral DNA and the inhibition of reproduction of DNA-containing viruses by the interaction of the viral DNA and DNase inside pinocytic vacuoles which protect the cells from the enzyme damaging effect.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo / Q000187:drug effects / Q000201:enzymology*; D003851:Deoxyribonucleases / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D006735:Horseradish Peroxidase / Q000494:pharmacology; D008854:Microscopy, Electron; D010179:Pancreas / Q000201:enzymology; D010873:Pinocytosis / Q000187:drug effects
D006735:Horseradish Peroxidase; D003851:Deoxyribonucleases
Elektronno-mikroskopicheskoe issledovanie proniknoveniia dezoksiribonukleazy v fibroblasti kurinykh émbrionov i vliianie fermenta na kletki.