Experimentally induced transplantation tolerance. I. Dissociation of effector lymphocytes specific for alloantigens by neonatal tolerance induction.
Neonatal tolerance was induced in CBA mice to alloantigens expressed by lymphoid cells of the A/Sn strain. 50% of the grafted animals maintained viable skin grafts for greater than 120 days and thus were considered operationally tolerant. However, splenic lymphocytes from these animals were capable of in vitro activation of DNA synthesis in response to the tolerizing alloantigens. Likewise, the spleen contained precursors of cytotoxic effector cells capable, upon activation, of similarly recognizing the major histocompatibility complex alloantigens used for tolerance induction. In contrast, in vivo graft-versus-host (GVH) effector function was absent, suggesting a possible dissociation of cells involved in the in vitro cell-mediated lympholysis and the in vivo GVH. Determination of potential serum-blocking factors as a possible mechanism of graft survival in these tolerant animals proved negative.
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D006082:Graft Enhancement, Immunologic; D006085:Graft Survival; D006087:Graft vs Host Reaction; D007108:Immune Tolerance; D007519:Isoantigens / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA
D007519:Isoantigens