Phytohaemagglutinin-induced leukocyte migration inhibition and phytohaemagglutinin skin test for screening the cell-mediated immune response in children.
Phytohaemagglutinin-induced leukocyte migration inhibition was studied in 72 children and compared with phytohaemagglutinin skin testing. The methods were used for screening the cell-mediated immune response. The in vitro method, which requires only 10-15% of the number of migrating cells as compared with other migration techniques, proved to be more sensitive, but gave some false-positive results when compared with other immunological parameters. Nevertheless, application of the microdroplet leukocyte migration is advantageous, used either simultaneously with the skin test, or as a single screening method in prematures, mature newborns and young infants, where application and/or evaluation of the skin tests is difficult.
D000293:Adolescent; D002464:Cell Migration Inhibition; D002648:Child; D002675:Child, Preschool; D004314:Down Syndrome / Q000150:complications; D006801:Humans; D007111:Immunity, Cellular; D007153:Immunologic Deficiency Syndromes / Q000150:complications / Q000175:diagnosis; D007223:Infant; D007231:Infant, Newborn; D007962:Leukocytes / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000150:complications; D010835:Phytohemagglutinins / Q000494:pharmacology*; D012882:Skin Tests; D013950:Thymus Gland / Q000002:abnormalities
D010835:Phytohemagglutinins