Growth-inhibitory glycopeptides obtained from the cell surface of cultured chick embryo fibroblasts.
Cell surface glycopeptides were obtained from cultured chick embryo fibroblasts (CEF) by digestion with Pronase E, and a fraction exerting growth-inhibitory activity on CEF was isolated by high performance gel permeation chromatography. The active fraction, tentatively termed cell surface glycopeptide-2 (CSGP-2), was soluble in 5% trichloroacetic acid (TCA) or 75% ethanol. It inhibited the growth of CEF reversibly at 10-20 micrograms sugar/ml, but did not inhibit BALB/c mouse 3T3, SV40-transformed 3T3, and human diploid cells at similar concentration. The growth-inhibitory activity of CSGP-2 was reduced or lost after digestion with neuraminidase or oxidation with sodium metaperiodate. Cellulose acetate electrophoresis revealed that CSGP-2 was a mixture of sialoglycopeptides. A similar growth inhibitor was also isolated from chicken embryonic tissues.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000032:analysis; D002478:Cells, Cultured; D002642:Chick Embryo; D002851:Chromatography, High Pressure Liquid; D004588:Electrophoresis, Cellulose Acetate; D005347:Fibroblasts / Q000032:analysis; D006131:Growth Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008970:Molecular Weight; D011402:Pronase; D014316:Tritium
D006131:Growth Inhibitors; D008565:Membrane Proteins; C031717:growth inhibitory proteins; D014316:Tritium; D011402:Pronase