Ouabain-resistant, frusemide-sensitive sodium efflux in human lymphocytes: a comparison of normotensive and hypertensive subjects.
Peripheral blood lymphocytes were isolated at room temperature in a low sodium medium in 17 normotensive control subjects (NC) and 13 patients with essential hypertension (EHP). Lymphocyte sodium content was not significantly different between NC and EHP (3.1 +/- 0.5, n = 11, and 3.6 +/- 0.5, n = 10, respectively; means +/- SEM). The effect of frusemide on the ouabain-resistant rate constant of sodium efflux (KNao) from these lymphocytes into a sodium-free medium was measured. The frusemide-sensitive efflux rate constant was 75 +/- mus (n = 17) in controls and significantly lower in EHP (32 +/- 7 mus, n = 13, P less than 0.001). In human lymphocytes there is a ouabain-resistant, frusemide-sensitive fraction of sodium efflux into a sodium-free medium; this fraction may be reasonably attributed to a sodium-potassium cotransport system. This sodium transport pathway seems to be reduced in lymphocytes from EHP.
D000328:Adult; D001693:Biological Transport, Active / Q000187:drug effects; D005260:Female; D005665:Furosemide / Q000494:pharmacology*; D006801:Humans; D006973:Hypertension / Q000097:blood*; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D012964:Sodium / Q000097:blood*
D010042:Ouabain; D005665:Furosemide; D012964:Sodium