Deletion map of the Escherichia coli structural gene for alkaline phosphatase, phoA.
Lambda transducing phages containing portions of the phoA gene have been isolated and used to construct a deletion map of the phoA gene. The isolation of a plaque-forming lambda transducing phage carrying the entire phoA gene is also described. Two new methods for screening or selection of mutants that have altered levels of alkaline phosphatase activity are reported.
D000469:Alkaline Phosphatase / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D009154:Mutation; D011995:Recombination, Genetic; D014161:Transduction, Genetic
D000469:Alkaline Phosphatase