[Nonprecipitability of the nucleic factor in the ultracentrifugation of tumor ascitic fluid].
Samples of cell-free ascitic liquid of mice and rats suffering from Ehrlich tumor and Zajdela's hepatoma, respectively, were centrifuged at 70,000 g and 200,000 g for 1 hour. Nucleic acid preparations were then isolated by phenol deproteinization and analysed by 8% polyacrylamide gel electrophoresis with subsequent densitometry. Under such conditions, DNA of nucleic factor as well as minor RNA fractions failed to precipitate. These results show that viral particles, tumor cell-produced vesicles and other large complexes do not incorporate DNA nucleic factor or minor RNA fractions.
D000818:Animals; D001202:Ascitic Fluid; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis*; D004273:DNA, Neoplasm / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D008114:Liver Neoplasms, Experimental / Q000032:analysis*; D051379:Mice; D008970:Molecular Weight; D009368:Neoplasm Transplantation; D012334:RNA, Neoplasm / Q000302:isolation & purification*; D051381:Rats; D014461:Ultracentrifugation
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm
Neosazhdaemost' nukleinovogo faktora pri ul'tratsentrifugirovanii astsiticheskoĭ zhidkosti opukholeĭ.