Effect of mercaptoethylamine on DNA degradation in thermophilic bacteria bac. Stearothermophilus exposed to gamma-, UV-radiation or methylnitrosourea.
The effect of mercaptoethylamine (MEA) on degradation of DNA in thermophilic bacteria Bac. stear. exposed to gamma-, UV-rays or methylnitrosourea (MNU) was studied. Using centrifugation on alkaline and neutral sucrose gradients, it was shown that MEA inhibits the accumulation of breaks in the DNA of Bac. stear. It also lowers the level of DNA degradation in toluene-treated cells of Bac. stear. under the action of the intrinsic nuclease, reduces the activity of the endonuclease specific for apurinic DNA, as well as that of S1-nuclease and DNase-I in vitro. The inhibition in the accumulation of DNA breaks is assumed to be due to a decrease of the endonuclease activity in the cells of thermophilic bacteria.
D003037:Cobalt Radioisotopes; D004269:DNA, Bacterial / Q000528:radiation effects*; D005720:Gamma Rays; D001411:Geobacillus stearothermophilus / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D008624:Mercaptoethylamines / Q000494:pharmacology*; D008770:Methylnitrosourea / Q000494:pharmacology*; D009607:Nitrosourea Compounds / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays
D003037:Cobalt Radioisotopes; D004269:DNA, Bacterial; D008624:Mercaptoethylamines; D009607:Nitrosourea Compounds; D011837:Radiation-Protective Agents; D008770:Methylnitrosourea