Passive enhancement of rat heart allografts by xenogeneic antidonor sera raised in rabbits.
Antilymphocyte serum (ALS) was raised in rabbits immunized by administration of lymphoid cells from Sprague-Dawley (SpD) rats. The ALS was rendered specific for the SpD strain by complete absorption by Wistar rat lymphoid cells. The specificity was considered to be predominantly directed to SpD Ir gene-associated (Ia) antigens, since absorption by SpD erythrocytes failed to abolish lymphocytotoxic activities. Alloantibody production by Wistar rats sensitized with SpD spleen cells was completely inhibited by simultaneous injections of SpD-specific ALS. This effect was specific as normal alloantibody production occurred when Wistar rats were immunized with ACI cells, as a third party. Although this ALS exhibited restricted enhancing ability on skin allograft survival, it was capable of significantly prolonging the survival of heart allografts from SpD to Wistar rats. The ALS showed blocking activities on mixed lymphocyte culture (MLC) but had no effect on cell-mediated cytotoxicity (CMC). The enhancing activity was thus considered to be mediated by an afferent blockage of immune responses.
D000042:Absorption; D000818:Animals; D000917:Antibody Formation; D000961:Antilymphocyte Serum / Q000627:therapeutic use*; D003602:Cytotoxicity, Immunologic; D004912:Erythrocytes / Q000276:immunology; D006082:Graft Enhancement, Immunologic; D006085:Graft Survival; D016027:Heart Transplantation; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000145:classification / Q000276:immunology; D008297:Male; D009115:Muridae; D011817:Rabbits; D051381:Rats; D011912:Rats, Inbred ACI; D011919:Rats, Inbred Strains; D016038:Skin Transplantation; D013045:Species Specificity
D000961:Antilymphocyte Serum; D007166:Immunosuppressive Agents