15N NMR studies of nitrogen metabolism in intact mycelia of Neurospora crassa.
Suspensions of intact mycelia of Neurospora crassa grown in medium containing 15NH4Cl have been found to give well-resolved 15N nuclear magnetic resonance spectra for metabolites that play crucial roles in intermediary nitrogen metabolism. These include the amide nitrogen of glutamine, the alpha-amino nitrogens of glutamate and other amino acids, the guanidino nitrogens of arginine, the ureido nitrogen of citrulline, the side-chain nitrogens of ornithine or lysine, or both, and uridine diphosphates. The turnover time of glutamine in vivo was estimated to be less than 1 hr by using nuclear magnetic resonance spectroscopy in conjunction with tracer methodologies. Applications of these techniques to the study of nitrogen metabolism are discussed.
D000596:Amino Acids / Q000096:biosynthesis; D000643:Ammonium Chloride / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000187:drug effects / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D009587:Nitrogen Isotopes; D014532:Uridine Diphosphate Glucose / Q000096:biosynthesis; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000096:biosynthesis
D000596:Amino Acids; D009587:Nitrogen Isotopes; D000643:Ammonium Chloride; D014537:Uridine Diphosphate N-Acetylglucosamine; D003513:Cycloheximide; D009584:Nitrogen; D014532:Uridine Diphosphate Glucose