[In vitro chemosensitivity test of human solid cancer: 3H-thymidine uptake inhibition test].
In vitro chemosensitivity test of human solid cancers, serially transplanted into nude mice was performed by detecting the inhibitory rate of 3H-thymidine incorporation to cancer cells. And the results of in vitro chemosensitivity test were compared with those in nude mice. This in vitro chemosensitivity test could present the quantitative results from a relatively small number of cancer cells within a short term (48 hours). Additionally, many samples could be handled at the same time by using microtestplates and a semiautomatic multiple cell harvester. The predictability of this in vitro chemosensitivity test to the in vivo effects was 80.6% (29/36) with 3 false positive and 4 false negative cases.
D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004341:Drug Evaluation / Q000379:methods; D004351:Drug Resistance; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013274:Stomach Neoplasms / Q000473:pathology*; D013936:Thymidine / Q000378:metabolism
D000970:Antineoplastic Agents; D013936:Thymidine