The effect of ethanol upon early development in mice and rats. II. In vitro effect upon early postimplantation rat embryos.
The direct effect of ethanol upon in vitro cultured 9.5 day rat embryos was investigated (2, 4, 8 and 10% ethanol added to the culture medium). The main effects recorded were as follows: 1. Significant increase of the number of "dying" embryos (beating heart without yolk sac circulation); 2. No significant increase of mortality; 3. Significant increase of the number of living embryos with deficient blood circulation; 4. Significant retardation of coiling in living embryos with a significant dose-effect relation, when the effects of 20/00 and 80/00 ethanol were compared; 5. Lowering of the mean somite number in living embryos; 6. Various macro- and macroscopical pathological changes (mainly necrotic areas in the central nervous system).
D000818:Animals; D001775:Blood Circulation / Q000187:drug effects; D001921:Brain / Q000196:embryology; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects*; D000431:Ethanol / Q000494:pharmacology*; D009336:Necrosis; D051381:Rats
D000431:Ethanol