Separation of elastin components by thin layer chromatography and electrophoresis.
Several methods are described which employ thin layer chromatography and electrophoresis for investigations into the structure and synthesis of crosslinked elastin. These procedures are more sensitive and involve less elaborate equipment than separation on paper. The methods described include an electrophoretic procedure for detecting the cross-linking amino acids in elastin hydrolysates, a method for completely separating desmosine, isodesmosine and merodesmosine in these hydrolysates, and a method for producing two-dimensional peptide maps of elastase digests of elastin.
D000596:Amino Acids / Q000302:isolation & purification*; D000818:Animals; D002855:Chromatography, Thin Layer / Q000379:methods; D003895:Desmosine / Q000032:analysis; D004549:Elastin / Q000032:analysis*; D004590:Electrophoresis, Paper / Q000379:methods; D007524:Isodesmosine / Q000032:analysis; D008022:Ligaments / Q000032:analysis*
D000596:Amino Acids; D003895:Desmosine; D004549:Elastin; D007524:Isodesmosine