Identification of 16-dehydropregnenolone as an intermediate in 16-androstene biosynthesis in neonatal porcine testicular microsomes.
The biosynthesis of 16-androstenes has been studied in neonatal porcine testicular microsomes using 17-hydroxypregnenolone and 16-dehydropregnenolone, separately, as substrates. The metabolites formed after microsomal incubation with these substrates were purified, derivatized as O-methyloxime-trimethylsilyl ethers and analysed by capillary gas chromatography-mass spectrometry. In the incubation of 17-hydroxypregnenolone with microsomes, 16-dehydropregnenolone was identified as an intermediate in the biosynthesis of 16-androstenes. Further microsomal incubation of 16-dehydropregnenolone has established the intermediary role of this steroid in the production of 16-androstenes.
D006907:17-alpha-Hydroxypregnenolone / Q000378:metabolism; D000736:Androstenes / Q000096:biosynthesis*; D000818:Animals; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D008861:Microsomes / Q000378:metabolism*; D011284:Pregnenolone / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism; D013737:Testis / Q000378:metabolism*
D000736:Androstenes; C042468:16-androstene; D006907:17-alpha-Hydroxypregnenolone; C003592:16-dehydropregnenolone; D011284:Pregnenolone