Lymphocyte glucocorticoid receptors in asthmatic and control subjects.
Glucocorticoid hormones, which are widely used in the treatment of asthma, have been shown to potentiate physiological and biochemical beta-adrenergic responsiveness in asthmatics. These effects are presumably mediated through glucocorticoid receptors. In order to better understand glucocorticoid pharmacology in asthmatics, we assayed glucocorticoid receptors by directly binding a radioactively labelled glucocorticoid hormone, dexamethasone, to intact lymphocytes prepared from the peripheral blood of asthmatics and control subjects. Binding studies were performed with dexamethasone at 100 nM and 5 nM concentrations. At 100 nM dexamethasone, the mean number of lymphocyte glucocorticoid receptors (per cell) in control subjects (7191 +/- 385, n = 9) was not significantly different from that in asthmatic subjects (7772 +/- 437, n = 9). At 5 nM dexamethasone, the mean number of glucocorticoid receptors in control subjects (1177 +/- 194, n = 5) was not significantly different from that in asthmatic subjects (1215 +/- 108, n = 8). At 100 nM dexamethasone, males had significantly more receptors (7939 +/- 360, n = 11) than females (6764 +/- 72, n = 7). Our results suggest that the number of lymphocyte glucocorticoid receptors and the apparent affinity of dexamethasone for receptors are not related to the presence of severity of asthma; however, a significant sex effect exists which should be corrected for in future studies of lymphocyte glucocorticoid receptors.
D000293:Adolescent; D000328:Adult; D001249:Asthma / Q000378:metabolism*; D003907:Dexamethasone / Q000378:metabolism; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D011965:Receptors, Glucocorticoid / Q000032:analysis* / Q000378:metabolism; D011987:Receptors, Steroid / Q000032:analysis*; D012737:Sex Factors
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D003907:Dexamethasone