Immunological cross-reactivity between large and small (2'-5')oligoadenylate synthetases from pig cells.
Using glycerol gradient centrifugation, the molecular sizes of porcine (2'-5')oligoadenylate synthetases (2-5A synthetases) were estimated. The 2-5A synthetase purified from pig spleen was about 150 kDa, while the enzyme extracted from nuclei of Newcastle disease virus-infected pig epithelial cells (SK-h) was about 20-40 kDa. The nuclear 2-5A synthetase was selectively adsorbed to Protein A-Sepharose beads conjugated with anti-spleen 2-5A synthetase antibody. Thus, the smaller 2-5A synthetase in nuclei of pig cells shares a protein structure with the larger enzyme from pig spleen.
D015088:2',5'-Oligoadenylate Synthetase / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D000936:Antigen-Antibody Complex; D003429:Cross Reactions; D007106:Immune Sera; D007527:Isoenzymes / Q000276:immunology* / Q000302:isolation & purification; D007700:Kinetics; D013154:Spleen / Q000201:enzymology; D013552:Swine
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007527:Isoenzymes; D015088:2',5'-Oligoadenylate Synthetase