[Mixed culture of sperm and lymphocyte in infertility with unexplained cause and its clinical implications (author's transl)].
Mixed culture of sperm and lymphocytes were performed in the samples of 32 infertile couples with unexplained cause, 16 couples with proven fertility and 47 virginal women in order to investigate the role of cellular-immunologic factors in infertility. 1) The means of stimulation index (S.I.) and the standard deviation in mixed cultures with donor's sperm were, 2.51 +/- 1.08 in infertile, 2.25 +/- 0.79 in fertile and 1.96 +/- 0.71 in virgin group. The S.I. of the virgin group was significantly lower (p less than 0.01) than that of the other two groups. 2) The means of S.I. in mixed cultures with husband's sperm were, 2.96 +/- 1.43 in infertile and 2.44 +/- 1.24 in fertile women. There was no significance in the S.I. of these two groups. 3) It was found during two years' prospective observation that 14 of 32 unexplainedly infertile couples achieved pregnancy. Retrospective analysis disclosed that there was no significant difference between pregnant and non-pregnant groups, when S.I. with husband's sperm were compared. Neither, no difference between S.I. with husband's sperm and donor's sperm was revealed in non-pregnant group. In conclusion, no evidence was obtained that unexplainedly infertile women, as a group, were suffered from immunologic factors.
D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology; D013094:Spermatozoa / Q000276:immunology*
D010835:Phytohemagglutinins