Regulation of fibrinogen synthesis by plasmin-derived fragments of fibrinogen and fibrin: an indirect feedback pathway.
The effect of plasmin-derived fibrinogen fragments on the biosynthesis of fibrinogen was investigated in cultured monolayers of rat hepatocytes. Incubating the cells with several concentrations of either fibrinogen or fibrin fragment D or E had no effect on the synthesis and secretion of fibrinogen by these cells. However, if the fragments were incubated with isolated peripheral blood leukocytes, they caused these cells to secrete a factor that when added to the hepatocytes caused an increase in fibrinogen synthesis 4- to 6-fold over controls. Moreover, the hepatocyte-stimulating factor also affected the production of several other proteins produced by the hepatocyte. These results demonstrate that both fragments D and E can stimulate hepatic fibrinogen synthesis via an indirect leukocyte-mediated pathway.
D000818:Animals; D002478:Cells, Cultured; D005246:Feedback; D005338:Fibrin Fibrinogen Degradation Products / Q000494:pharmacology*; D005340:Fibrinogen / Q000096:biosynthesis*; D005341:Fibrinolysin / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology*; D008099:Liver / Q000378:metabolism*; D051381:Rats
D005338:Fibrin Fibrinogen Degradation Products; D005340:Fibrinogen; D005341:Fibrinolysin