The MLC response of lymphocytes from mice neonatally primed and tolerating skin allografts.
Newborn B10.A mice were primed intravenously with semiallogeneic spleen cells differing in the whole H-2 complex or in individual regions of H-2. Neonatally treated mice were grafted with respective skin allografts and cells from tolerant animals were tested in mixed lymphocyte culture. These cells were found to be a reactive or hyporeactive to MLC-stimulating determinants. Using a panel of restimulating cells, MLC tolerance was found to be immunologically specific. Lymphocytes from tolerant mice did not react only to the alloantigens used for neonatal priming, whereas they showed similar reactions to third party antigens as did lymphocytes from normal untreated mice. The results indicate that neonatal priming leads to the tolerance which is manifested already in the recognitive phase of the allotransplantation reaction and that this MCL tolerance is specific, at least in B10.A mice.
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D006085:Graft Survival; D006183:H-2 Antigens / Q000032:analysis; D007108:Immune Tolerance; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008815:Mice, Inbred Strains; D016038:Skin Transplantation; D013154:Spleen / Q000276:immunology / Q000637:transplantation; D014181:Transplantation Immunology
D006183:H-2 Antigens