Acetaldehyde formation by mitochondria from the free-living nematode Panagrellus redivivus.
The mitochondrial fraction from the free-living nematode Panagrellus redivivus decarboxylates pyruvate to produce significant amounts of acetaldehyde. Acetaldehyde formation is stimulated by thiamin pyrophosphate, shows a sharp optimum at pH 6.8 and is greater under anaerobic than aerobic conditions. The pyruvate decarboxylase activity cofractionates with, and is probably a partial reaction of, the pyruvate dehydrogenase complex. Acetaldehyde production is modulated by NAD+, ATP and acetyl-CoA and is greatly stimulated by lipoic acid. The pyruvate decarboxylase system is extremely sensitive to thiol-group inhibitors and is inhibited by oxygen in the presence of pyruvate.
D000079:Acetaldehyde / Q000378:metabolism*; D000227:Adenine Nucleotides / Q000494:pharmacology; D000693:Anaerobiosis; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D005740:Gases; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D009348:Nematoda / Q000187:drug effects / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology; D011768:Pyruvate Dehydrogenase Complex / Q000302:isolation & purification
D000227:Adenine Nucleotides; D000963:Antimetabolites; D005740:Gases; D011506:Proteins; D011725:Pyridines; D011768:Pyruvate Dehydrogenase Complex; D000079:Acetaldehyde