Effect of 2-mercaptopropionylglycine on lipid peroxidation and drug oxidation in rat liver microsomes.
2-Mercaptopropionylglycine, a synthetic thiol, significantly stimulated NADPH-dependent lipid peroxidation by rat liver microsomes, while the thiol inhibited the microsomal aminopyrine N-demethylase activity with an increase in lipid peroxidation. But, a strong inhibition of lipid peroxidation by EDTA could not abolish the inhibition of the N-demethylase activity by the thiol. Besides, the thiol markedly increased not only the Km value for aminopyrine N-demethylase but also the apparent Ks value for aminopyrine binding to the microsomal oxidized cytochrome P-450 by interacting with the cytochrome P-450.
D000603:Amino Acids, Sulfur / Q000494:pharmacology*; D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D007700:Kinetics; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D008625:Tiopronin / Q000494:pharmacology*
D000603:Amino Acids, Sulfur; D008054:Lipid Peroxides; D008315:Malondialdehyde; D003577:Cytochrome P-450 Enzyme System; D008625:Tiopronin; D000633:Aminopyrine N-Demethylase