The monoclonal antibody HNK-1 reacts with a human peripheral nerve ganglioside.
The monoclonal HNK-1 antibody, a marker for human natural killer cells, strongly reacted with human peripheral nerve gangliosides in the enzyme-linked immunosorbent assay. Autoradiography after the binding of HNK-1 to thin-layer chromatograms of peripheral nerve gangliosides followed by radioiodinated goat anti-mouse IgM revealed that HNK-1 was reacting with a minor ganglioside that chromatographed between GM1 and GD1a. The antigen was insensitive to digestion with neuraminidase and pronase.
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D001923:Brain Chemistry; D002855:Chromatography, Thin Layer; D004797:Enzyme-Linked Immunosorbent Assay; D005732:Gangliosides / Q000032:analysis* / Q000276:immunology; D006801:Humans; D009439:Neuraminidase; D011402:Pronase; D012584:Sciatic Nerve / Q000032:analysis*; D013116:Spinal Cord / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D005732:Gangliosides; D009439:Neuraminidase; D011402:Pronase