The effect of bilipolinum (Adipiodon), an iodine contrast medium on erythrocyte enzymes.
Bilipolinum (Adipiodon), iodine contrast medium used in cholangiography, showed an inhibitory effect on the activity of human erythrocyte phosphohexoseisomerase, phosphofructokinase, aldolase and glucose-6-phosphate dehydrogenase. The addition of glucose metabolites (glucose-6-phosphate, fructose-6-phosphate, fructose-1,6-bis-phosphate, pyruvate and lactate) abolished the inhibitory effect of Bilipolinum. In the presence of Bilipolinum purified erythrocyte phosphofructokinase showed a decreased affinity towards substrate, modified allosteric properties and reduced stability at pH below 7.5. Purified erythrocyte glucose-6-phosphate dehydrogenase was also affected by Bilipolinum and its affinity for NADP was decreased. Testing of erythrocyte enzymes in the evaluation of toxicity of iodine contrast media is discussed.
D003287:Contrast Media / Q000494:pharmacology*; D004912:Erythrocytes / Q000201:enzymology*; D005634:Fructose-Bisphosphate Aldolase / Q000097:blood; D005956:Glucose-6-Phosphate Isomerase / Q000097:blood; D005954:Glucosephosphate Dehydrogenase / Q000097:blood; D006801:Humans; D007458:Iodipamide / Q000494:pharmacology*; D010732:Phosphofructokinase-1 / Q000097:blood
D003287:Contrast Media; D005954:Glucosephosphate Dehydrogenase; D010732:Phosphofructokinase-1; D005634:Fructose-Bisphosphate Aldolase; D005956:Glucose-6-Phosphate Isomerase; D007458:Iodipamide