The identification of outer membrane proteins and flagella of Campylobacter jejuni.
The outer membrane proteins of five clinical isolates of Campylobacter jejuni were identified by 125I-surface labelling and SDS-PAGE of outer membrane preparations. All isolates expressed a major outer membrane protein of variable molecular weight (43 000-46 000: 43K-46K). Several constant surface proteins were also identified including a 27K protein which was surface-exposed and acid-extractable but was not present in the outer membrane preparations. Isolated flagella comprised a major 62K protein and a minor 87K protein. Both proteins were absent in an aflagellate variant. The 62K protein was immunoblotted and immunoprecipitated by rabbit anti-flagella antisera.
D001345:Autoradiography; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000032:analysis*; D002168:Campylobacter fetus / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005407:Flagella / Q000032:analysis; D005408:Flagellin / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis*; D008970:Molecular Weight
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D008565:Membrane Proteins; D005408:Flagellin