DNA damage by haloalkanes in human lymphocytes cultured in vitro.
The DNA damaging activity of 7 haloalkanes was studied in a short-term in vitro system which utilized human lymphocytes. The parameters studied were the inhibition of scheduled (duplicative) and unscheduled (reparative) DNA syntheses seen as tritiated thymidine uptake. The results obtained suggested that chloromethyl methyl ether (CMME), 1,2-dibromoethane (DBE), trichloroethylene (TCE) and 1,2-dichloroethane (DCE) gave positive results such as DNA damaging agents, while carbon tetrachloride (CTC), chloroform (TCM) and dichloromethane (DCM) gave low or negative results.
D002251:Carbon Tetrachloride / Q000494:pharmacology; D002273:Carcinogens; D002478:Cells, Cultured; D002725:Chloroform / Q000494:pharmacology; D004247:DNA / Q000037:antagonists & inhibitors*; D004260:DNA Repair / Q000187:drug effects; D015946:Ethylene Dibromide / Q000494:pharmacology; D005025:Ethylene Dichlorides / Q000494:pharmacology; D006801:Humans; D006838:Hydrocarbons / Q000494:pharmacology*; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects*; D008738:Methyl Ethers / Q000494:pharmacology; D008752:Methylene Chloride / Q000494:pharmacology; D014241:Trichloroethylene / Q000494:pharmacology
D002273:Carcinogens; D005025:Ethylene Dichlorides; D006838:Hydrocarbons; D008738:Methyl Ethers; D015946:Ethylene Dibromide; D014241:Trichloroethylene; D008752:Methylene Chloride; D002725:Chloroform; D004247:DNA; D002251:Carbon Tetrachloride