A multinuclear NMR study of 2,3-bisphosphoglycerate metabolism in the human erythrocyte.
Resonances from 13C, 31P and 1H have been detected simultaneously in suspensions of human erythrocytes using a modified NMR spectrometer equipped with a probe tuned to four different frequencies. The utility of multinuclear NMR in the study of cellular metabolism is demonstrated with an investigation of 13C label flux through the 2,3-bisphosphoglycerate bypass in human erythrocytes. In a single experiment, the respective contributions of this bypass and the pentose-phosphate shunt were found to be 27 and 10% of the total glycolytic rate.
D019794:2,3-Diphosphoglycerate; D001786:Blood Glucose / Q000032:analysis; D002247:Carbon Isotopes; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods
D001786:Blood Glucose; D002247:Carbon Isotopes; D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate