Inhibition of mitochondrial and soluble hexokinase from various rat tissues by glucose 1,6-bisphosphate.
Mitochondrial and soluble Type I and Type II hexokinase from various rat tissues differed in their susceptibility to inhibition by glucose-1,6-bisphosphate (Glc-1,6-P2). In tissues where Type I is the predominant form, the mitochondrial enzyme was less susceptible to inhibition by Glc-1,6-P2 than the soluble enzyme, especially at high Mg2+ concentration. In tissues where Type II is the predominant form, the mitochondrial enzyme was more susceptible to inhibition by Glc-1,6-P2 than the soluble enzyme, especially at low Mg2+ concentration. The results suggest that changes in the intracellular concentrations of Glc-1,6-P2 and Mg2+ under various conditions would affect the activity of the bound and soluble hexokinase from different tissues in a different manner.
D000273:Adipose Tissue / Q000201:enzymology; D000367:Age Factors; D000818:Animals; D001921:Brain / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives*; D005958:Glucosephosphates / Q000494:pharmacology*; D006593:Hexokinase / Q000037:antagonists & inhibitors*; D008274:Magnesium / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology; D009132:Muscles / Q000201:enzymology; D051381:Rats; D012867:Skin / Q000201:enzymology
D005958:Glucosephosphates; D019298:Glucose-6-Phosphate; C016473:glucose-1,6-bisphosphate; D006593:Hexokinase; D008274:Magnesium