Biological activities of analogues of lipid A based chemically on the revised structural model. Comparison of mediator-inducing, immunomodulating and endotoxic activities.
Lipid A analogues were chemically synthesized based on the model structure recently revised, and biological activities of the analogues were tested. The analogue, (beta-1,6)-linked glucosamine disaccharide carrying ester-bound 3-hydroxytetradecanoic acids at 3 and 3' position of reducing and nonreducing glucosamine in addition to amide-bound 3-hydroxytetradecanoic acids and glycosidic-linked and ester-linked phosphate groups, showed much stronger activities for mediator inducing and immunomodulating as well as endotoxic activities than those exhibited by the previously synthesized analogues based on the old model. Among the activities tested, induction of interferon and tumor necrosis factor as well as mitogenicity, adjuvanticity and pyrogenicity were, however, not expressed so strongly as natural lipid A used as controls. In contrast, the analogue exhibited comparable activities to those of control lipid A in the test of lethal toxicity to mice and gelating activity of Limulus amebocyte lysate. Other synthetic analogues carrying a phosphate group showed comparable, slightly stronger or weaker activities depending on the test, but nonphosphorylated analogue exhibited no apparent or only very weak activities.
D000276:Adjuvants, Immunologic / Q000138:chemical synthesis; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003429:Cross Reactions; D004731:Endotoxins / Q000138:chemical synthesis; D005227:Fatty Acids / Q000302:isolation & purification; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis; D007369:Interferon Inducers / Q000138:chemical synthesis; D007928:Lethal Dose 50; D008033:Limulus Test; D008050:Lipid A / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000276:immunology / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens / Q000138:chemical synthesis; D011749:Pyrogens / Q000138:chemical synthesis; D011817:Rabbits; D013329:Structure-Activity Relationship; D014409:Tumor Necrosis Factor-alpha
D000276:Adjuvants, Immunologic; D004731:Endotoxins; D005227:Fatty Acids; D006023:Glycoproteins; D007369:Interferon Inducers; D008050:Lipid A; D008934:Mitogens; D011749:Pyrogens; D014409:Tumor Necrosis Factor-alpha