Monoclonal antibody to human platelet glycoprotein I. I. Immunological studies.
A monoclonal hybridoma antibody specific for platelet glycoprotein I complex is described. The nature od the antigen was determined by demonstration that it was chymotrypsin sensitive and gave a peak at 150 000 daltons on SDS-PAGE after immunoprecipitation. The expression of the antigen is restricted to platelets and megakaryocytes with at least 1.6 x 10(4) molecules of antigen per platelet. The antibody failed to bind to platelets from patients with Bernard Soulier syndrome, where there is known to be a deficiency of glycoprotein Ib/Is expression. Binding to platelets from patients with Glanzmann's thrombasthenia was normal.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000032:analysis; D001791:Blood Platelet Disorders / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D010980:Platelet Membrane Glycoproteins; D011233:Precipitin Tests; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D000941:Antigens; D006023:Glycoproteins; D008565:Membrane Proteins; D010980:Platelet Membrane Glycoproteins