Antigenicity of human fibrinogen and its derivatives.
Serological characterization of fibrinogen and its derivatives was attempted by using antibody-coated latex agglutination and counterelectrophoresis. It was found that: 1) fibrinogen was not only capable of agglutinating uncoated latex particles but also fixed to the latex particles; 2) the sensitivity of some of the latex reagents used for the detection of fibrinogen may reflect the antibody activity to the derivatives with altered antigenicity in which FDP-E antigen is more readily available than in intact fibrinogen molecules, as the one contained in Liquid Human Plasma but absent in its batches prepared in the presence of plasmin inhibitors; 3) increase in FDP-E antigenicity was noticed in the course of fibrinogenolysis so its availability would indicate the occurrence and extent of proteolytic process on fibrinogen; and 4) FDP-E-antigen developed in the course of plasmin digestion may not be shared by intact fibrinogen since the former was reactive in counterelectrophoresis against anti-FDP-E serum even when the latter had been absorbed with fibrinogen.
D000371:Agglutination; D000941:Antigens / Q000276:immunology*; D005338:Fibrin Fibrinogen Degradation Products / Q000276:immunology*; D005340:Fibrinogen / Q000276:immunology*; D005341:Fibrinolysin / Q000378:metabolism; D005342:Fibrinolysis; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D066298:In Vitro Techniques; D007841:Latex Fixation Tests
D000941:Antigens; D005338:Fibrin Fibrinogen Degradation Products; D007106:Immune Sera; D005340:Fibrinogen; D005341:Fibrinolysin