Thermospray liquid chromatography/mass spectrometry for the analysis of L-carnitine and its short-chain acyl derivatives.
A method utilizing thermospray high-performance liquid chromatography/mass spectrometry for the separation and direct analysis of carnitine, acetylcarnitine, and propionylcarnitine is described. On-column analysis of mixtures of the acylcarnitines with their corresponding stable, isotope-labeled analogs at nanomolar concentrations has indicated that isotope dilution assays can be applied towards the analysis of carnitine and short-chain acylcarnitines present in biological samples.
D000108:Acetylcarnitine / Q000032:analysis; D002331:Carnitine / Q000031:analogs & derivatives* / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D006358:Hot Temperature; D013058:Mass Spectrometry / Q000295:instrumentation
C003223:propionylcarnitine; D000108:Acetylcarnitine; D002331:Carnitine