[Clonage of the "malA" region of "Escherichia coli" K12: nucleotide sequence of the regulatory region and the promoters, identification and purification of the MalT-activator protein (author's transl)].
A 5,800-bp (base pair) HindIII-EcoRI DNA fragment containing malT, the positive regulator gene of the maltose regulon, and most of malP, the structural gene for maltodextrin phosphorylase, was cloned into pBR322. A sequence of 802 bp was established in a DNA segment containing the promotor for malPQ and the promoter for malT. A total of 611 bp separates the initiation codons for these two genes, which are transcribed in opposite directions. The malT product was identified as a 94,000 dalton polypeptide.
D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005964:Glucosyltransferases / Q000235:genetics*; D008970:Molecular Weight; D009876:Operon
D003062:Codon; D005964:Glucosyltransferases; C033938:maltodextrin phosphorylase
Clonage de la région malA de Escherichia coli K12: séquence des nucléotides des régions régulatrices et des promoteurs, identification et purification de la protéine activatrice MalT.