[Cell kinetics and radiosensitivity of cultured cow lymphocytes].
The harlequin-staining technique has been used to study, after PHA-stimulation, the cell proliferation of cow lymphocytes in culture and to assess the radiosensitivity in first mitosis cells. At the 48 h fixation time, only 34% of the cells are in first mitosis whereas 55% are already in second and 11% in third mitosis. The exposure of cow lymphocytes to 200 rad X-rays result in the production of 16% dicentric chromosomes in first mitosis cells.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D005260:Female; D007700:Kinetics; D008213:Lymphocyte Activation / Q000528:radiation effects; D008214:Lymphocytes / Q000502:physiology / Q000528:radiation effects*; D008677:Metaphase / Q000528:radiation effects; D008938:Mitosis / Q000528:radiation effects*; D010835:Phytohemagglutinins
D010835:Phytohemagglutinins
Cinétique cellulaire et radiosensibilité des lymphocytes de vache en culture.