Characterization of various immunoglobulin preparations for intravenous application. II. Complement activation and binding to staphylococcus protein A.
14 immunoglobulin preparations for intravenous use were tested to assess functions of their Fc portion. Inhibition of the hemolytic activity of complement, C1q binding and the interaction of IgG subclasses with Staphylococcus protein A were investigated. Complement studies were done on both the ready-for-infusion and the heat-aggregated preparations. Three groups of products could be distinguished: (1) Enzymatically and chemically treated products were devoid of complement-activating capacity, both when tested and ready-for-infusion and as heat-aggregated preparations. The chemically treated preparations showed atypical binding properties to Staphylococcal protein A. (2) The poly-(ethylene glycol) (PEG)-treated preparations and the untreated reference activated complement before and after heat-aggregation. (3) The albumin protected and the pH 4-treated product did not spontaneously activate complement in the ready-for-infusion state but did so after heat-aggregation. These results suggest that only the albumin-protected and the pH 4-treated products can be expected both to be well tolerated when given intravenously to high-risk agammaglobulinemic patients and to exhibit normal Fc functions in vivo.
D003166:Complement Activating Enzymes / Q000378:metabolism; D003167:Complement Activation / Q000187:drug effects; D015922:Complement C1q; D005341:Fibrinolysin / Q000494:pharmacology; D006461:Hemolysis; D006801:Humans; D007074:Immunoglobulin G / Q000145:classification / Q000378:metabolism*; D007136:Immunoglobulins / Q000276:immunology / Q000627:therapeutic use*; D007275:Injections, Intravenous; D010434:Pepsin A / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D013205:Staphylococcal Protein A
D007074:Immunoglobulin G; D007136:Immunoglobulins; D013205:Staphylococcal Protein A; D011092:Polyethylene Glycols; D015922:Complement C1q; D003166:Complement Activating Enzymes; D005341:Fibrinolysin; D010434:Pepsin A