Formation of elastic fibers and elastin in rabbit aortic smooth muscle cell cultures.
Cultured rabbit aortic smooth muscle cells produce elastic fibers and elastin in their extracellular matrix. Morphological analyses of elastic fibers indicate that they consist of two distinct components which play a major role in elastic early fiber formation namely, the amorphous component and the "microfibrillar" component. During elastogenesis, the early fiber consists of small bundles of filaments. Later, clearly distinguishable small conglomerates of amorphous material are found distributed among the bundles of filaments. The mature elastic fibers have large conglomerates of amorphous material with the filaments present within the interstices. Long term cultures of these cells in the second passage continue to accumulate elastin. The crosslinking amino acid isomers desmosine and isodesmosine, which are unique to insoluble elastin, increase with time in culture. Twenty-four hour pulses with 14C-proline followed by measurements of 14C-hydroxyproline in the collagenase resistant protein of the cell layer show that the synthesis of insoluble elastin is constant up to the 14 week period studied from the time the amorphous material becomes evident ultrastructurally.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001013:Aorta, Thoracic / Q000502:physiology* / Q000648:ultrastructure; D002478:Cells, Cultured; D004548:Elasticity; D004549:Elastin / Q000096:biosynthesis*; D007700:Kinetics; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular / Q000502:physiology / Q000648:ultrastructure; D011817:Rabbits
D000596:Amino Acids; D004549:Elastin