T lymphocytes involved in inhibition of granulopoiesis in two neutropenic patients are of the cytotoxic/suppressor (T3+T8+) subset.
T lymphocyte-mediated bone marrow failure is a recently recognized clinical disorder, but the T lymphocyte subsets responsible for mediating the inhibitory effect have not been identified. We obtained T lymphocytes from the bone marrow of two patients with T lymphocyte-mediated granulopoietic failure, exposed them to monoclonal antibodies (OKT3, OKT4, and OKT8) in cytotoxicity assays, then recombined the treated T cells with autologous T-depleted marrow cells in clonal assays for granulocyte/macrophage progenitors (CFU-GM). Treatment of T cells with OKT3 and OKT8 abrogated their granulopoietic inhibitory effect but treatment with OKT4 did not. Therefore, in these two patients, the lymphocytes that played a role in the inhibition of granulopoiesis were of the cytotoxic/suppressor subset.
D000380:Agranulocytosis / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D001853:Bone Marrow / Q000276:immunology / Q000473:pathology; D002469:Cell Separation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003602:Cytotoxicity, Immunologic; D005260:Female; D006098:Granulocytes / Q000473:pathology*; D006410:Hematopoiesis; D006461:Hemolysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D009503:Neutropenia / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000145:classification*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000911:Antibodies, Monoclonal