Dynamic laser light scattering studies of the micelle to vesicle transition in model and native bile.
Using quasielastic light scattering, we investigated the formation of vesicles produced spontaneously by diluting a mixed micellar solution of bile salt and lecithin beyond the micellar phase boundary. The vesicles were nearly monodisperse, and their size varied between 120 to 500 A depending upon the dilution conditions. A systematic study of the dependence of the vesicle size upon the bile salt and lecithin concentration was made and interpreted in terms of a simple equilibrium model. Likewise, the particle size distribution in native bile obtained from the dog was studied. A comparison of the dilution effect on native bile and on model bile of identical biliary lipid composition provides unambiguous evidence for micellar aggregates and the occurrence of a micelle to vesicle transition in native bile.
D000818:Animals; D001646:Bile; D001647:Bile Acids and Salts; D003102:Colloids; D004285:Dogs; D005260:Female; D007834:Lasers; D008823:Micelles; D010316:Particle Size; D010713:Phosphatidylcholines; D012542:Scattering, Radiation
D001647:Bile Acids and Salts; D003102:Colloids; D008823:Micelles; D010713:Phosphatidylcholines