Isolation of a DNA plasmid in the fungus Rhizoctonia solani.
A DNA plasmid, designated pRS64, was detected in three isolates of anastomosis group 4 (AG-4) of Rhizoctonia solani Kühn by biophysical methods. The plasmid was a linear double-stranded DNA with a molecular weight of 1.68 +/- 0.06 X 10(6) or 2617 +/- 87 bp. Weakly pathogenic isolates of R. solani, 1668 RI-1, 1271 RI-64 and 1272 RI-1, which showed abnormally slow growth, contained the plasmids, but pathogenic isolates, 1668, 1271 and 1272, showing normal growth, contained no detectable plasmid DNA.
D004271:DNA, Fungal / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D008854:Microscopy, Electron; D003904:Mitosporic Fungi / Q000032:analysis*; D008970:Molecular Weight; D010957:Plasmids; D012232:Rhizoctonia / Q000032:analysis*
D004271:DNA, Fungal