Cycloheximide-sensitive recovery from 254 nm UV light damage in cultured marsupial cells.
Colony-forming ability of Potorous tridactylus-kidney (PtK-2) cells was measured after exposure to 254-nm ultraviolet (UV) light and cycloheximide. Addition of 5 microM cycloheximide after exposure of the cells to UV light greatly decreased cell survival. Maximum effect of the drug was obtained by 24-h exposure after irradiation. The cycloheximide sensitivity of irradiated cells was eliminated if addition of the drug was delayed for 8-10 h after irradiation, or if the cells received photoreactivating light treatment prior to cycloheximide exposure. Thus, a major component of pyrimidine dimer removal may involve a cycloheximide-sensitive mechanism.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology*; D004260:DNA Repair / Q000187:drug effects*; D007668:Kidney; D008394:Marsupialia; D010777:Photochemistry; D011740:Pyrimidine Dimers / Q000378:metabolism; D014466:Ultraviolet Rays
D011740:Pyrimidine Dimers; D003513:Cycloheximide