[Characteristics of the primary and secondary DNA structure of staphylococcal phage SB-1].
Equilibrium centrifugation, spectral analysis of thermal denaturation and direct chemical determinations showed staphylococcal phage Sb-I DNA to be characterized by a standard set of nitric bases (28.5 mol.2./% G-C). No abnormal bases or other extracomponents were found. From the differential spectral analysis of melting interval it is concluded that G-C pairs are distributed along DNA molecule in a Gauss type. Spectrophotometric and thermodynamic parameters of melting show phage Sv-I DNA to have a typical double-stranded structure. DNA is characterized by enthalpies of conformational transitions of spiral=glome delta H=11.4 cal/g and delta H = 9.7 cal/g for 1 x SSC and 0.1 x SSC diluents, respectively.
D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004279:DNA, Viral; D006358:Hot Temperature; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D013057:Spectrum Analysis; D013204:Staphylococcus Phages / Q000032:analysis*; D013816:Thermodynamics
D004279:DNA, Viral
Kharakteristika pervichnoĭ i vtorichnoĭ struktury DNK stafilokokkovogo faga SB-1.