Poly(ADP-ribose) metabolism appears normal in EM9, a mutagen-sensitive mutant of CHO cells.
EM9 is a mutagen-sensitive CHO cell whose phenotype resembles that of normal CHO cells exposed to 3-aminobenzamide, an inhibitor of poly(ADP-ribose) synthesis. This phenotype suggested that EM9 might be defective in poly(ADP-ribose) metabolism, but we now cannot find any abnormality in the synthesis or in the degradation of poly(ADP-ribose) in permeabilized EM9 cells. Thus the effects of 3-aminobenzamide on wild-type cells may be due to the inhibition of processes other than poly(ADP-ribose) synthesis. 3-Aminobenzamide enhances the cytotoxicity of EMS toward EM9 and control cells to the same degree.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D003851:Deoxyribonucleases / Q000378:metabolism; D003857:Deoxyuridine / Q000031:analogs & derivatives / Q000633:toxicity; D003902:Detergents / Q000494:pharmacology; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D005260:Female; D007536:Isomerism; D007700:Kinetics; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D009243:NAD / Q000378:metabolism; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D017830:Octoxynol; D010053:Ovary; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology
D003902:Detergents; D008244:Lysophosphatidylcholines; D009153:Mutagens; D009702:Nucleoside Diphosphate Sugars; D009243:NAD; D011064:Poly Adenosine Diphosphate Ribose; D011092:Polyethylene Glycols; C037291:2'-chloro-2'-deoxyuridine; D017830:Octoxynol; D005020:Ethyl Methanesulfonate; D003851:Deoxyribonucleases; D003857:Deoxyuridine