Characterization of human growth hormone from acromegalic pituitary tumors.
Human growth hormone (HGH) was extracted from acromegalic pituitary tumors at pH 10.5 and precipitated with ammonium sulfate at 20-40% saturation. It was purified on a Sephadex G-100 column to yield monomeric HGH. The tumor-HGH was indistinguishable from the authentic one in polyacrylamide gel electrophoresis at pH 8.3 or in the presence of sodium dodecyl sulfate, high-performance liquid chromatography, radioimmunoassay, peptide map, amino acid composition and N-terminal partial amino acid sequence. The tumor-HGH is active in the tibia assay and bodyweight gain test in hypophysectomized rats with comparable potency to that of the authentic sample.
D000172:Acromegaly / Q000503:physiopathology*; D000596:Amino Acids / Q000032:analysis; D013006:Growth Hormone / Q000032:analysis*; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis; D010911:Pituitary Neoplasms / Q000032:analysis*
D000596:Amino Acids; D010446:Peptide Fragments; D013006:Growth Hormone