Representative applications of heparin-sepharose in the removal of polyamines from biological materials.
Many experimental systems would greatly benefit from the availability of a simple and effective technique to remove polyamines from biological materials. We have examined the possibility of utilizing heparin-sepharose in the removal of polyamines from rat heart mitochondria, DNA-spermine complex, and fetal calf serum. Heparin-sepharose removes 90% of spermine adsorbed to the cytoplasmic surface of rat heart mitochondria. Heparin-sepharose almost totally removes spermine from DNA-spermine complex, leaving less than 0.003 mol spermine/mol DNA phosphorus. Heparin-sepharose is highly effective in removing spermine and spermidine (99.5 and 95% adsorbed, respectively) from fetal calf serum. Under the same experimental conditions only 50% of putrescine is adsorbed. A higher amount of resin corresponding to an increased capacity for putrescine must be used to achieve a satisfactory removal of putrescine.
D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism; D005260:Female; D008297:Male; D008566:Membranes / Q000032:analysis; D008722:Methods; D008929:Mitochondria, Heart / Q000032:analysis; D011073:Polyamines / Q000097:blood / Q000302:isolation & purification*; D011247:Pregnancy; D011700:Putrescine / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D012685:Sepharose / Q000031:analogs & derivatives*; D013095:Spermidine / Q000302:isolation & purification; D013096:Spermine / Q000302:isolation & purification
D011073:Polyamines; C037405:heparin-sepharose; D013096:Spermine; D004247:DNA; D012685:Sepharose; D013095:Spermidine; D011700:Putrescine