The role of germinal vesicle in maturation of Pleurodeles waltlii oocytes induced by steroids.
The maturation of oocytes from unstimulated Pleurodeles waltlii females can be induced in vitro. All full-grown oocytes covered by follicular envelopes mature under the influence of progesterone, testosterone, and a synthetic steroid (CDMT). Enucleation of oocytes prior to maturation induction resulted in a significantly lower percentage of maturation. Irradiation, or the treatment of oocytes with actinomycin D, did not affect their maturation. Therefore the lower percentage of maturation after enucleation must be due to the lack of karyoplasm. Karyoplasm appears to be necessary for the production of the maturation-promoting factor in P. waltlii oocytes.
D000818:Animals; D002467:Cell Nucleus / Q000502:physiology*; D003593:Cytoplasm / Q000502:physiology; D003609:Dactinomycin / Q000494:pharmacology; D005260:Female; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development* / Q000528:radiation effects; D006080:Ovarian Follicle / Q000502:physiology; D010999:Pleurodeles; D011374:Progesterone / Q000494:pharmacology*; D013739:Testosterone / Q000031:analogs & derivatives* / Q000494:pharmacology*
D003609:Dactinomycin; C000430:D-4-chloro-17 beta-hydroxy-3-oxo-17 alpha-methylandrosta-1,4-diene; D013739:Testosterone; D011374:Progesterone