Polyclonal activation of Ts cells with antiserum directed against an IGH-1 linked candidate for a T-cell receptor constant region marker.
An anti-T cell serum raised in allotype congenic mice recognizes the product of a new locus coding for a heavy chain-linked polypeptide found on a subpopulation of T cells. Anti-Tsd raised in BALB/cAnN mice against selected C.AL-20 T cells reacts with a cell surface antigen in virgin animals that is found on 25% of mature thymocytes and Lyt-bearing T cells, but not on prothymocytes, Lyt1 T cells or B cells. The antigen is restricted to strains bearing the Ig-1d and Ig-1e heavy chain allotype haplotypes, and is expressed in the F1 animal. The antigen is unlinked in expression to the Lyt2, H-2, or kappa light chain loci. The antigen is not detected in the hematopoietic cells in the bone marrow and appears to mark only the mature peripheral pool of T cells. As previously reported, the antiserum blocks the binding of suppressor T cells to the cross-reactive idiotype for arsonate, while reagents specific for Fab, Fc and Ig were ineffective. It seems probable that the marker may represent a T cell constant region marker analogous to the Igh products on immunoglobulin. Antiserum against this marker induces in vivo triggering of Ts cells for a wide variety of T-dependent antigens. All subclasses of anti-hapten antibodies are suppressed; no affinity restrictions or clonotype specificity is observed in suppressed adult mice. Results suggest that precursor T cells regulating major serum idiotypes regulate individual idiotypes.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D007106:Immune Sera; D007126:Immunoglobulin Allotypes / Q000276:immunology; D007127:Immunoglobulin Constant Regions / Q000276:immunology*; D007075:Immunoglobulin M; D007136:Immunoglobulins / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D011971:Receptors, Immunologic / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000954:Antigens, Surface; D007106:Immune Sera; D007126:Immunoglobulin Allotypes; D007127:Immunoglobulin Constant Regions; D007075:Immunoglobulin M; D007136:Immunoglobulins; D011971:Receptors, Immunologic