[Uptake of radioactive precursors for the evaluation of sensitivity of anticancer drugs against cancer cells].
An in vitro drug sensitivity test was developed to estimate the lethal effects of drugs on cancer cells. Cancer cells we incubated in the presence of radioactive precursors and anticancer drugs. The drug effects were estimated from the changes in rates of incorporation of precursors into DNA, RNA, and protein. Handling of a large number of samples became possible using a microtiter plate and a multiple automatic cell harvester. Incorporation of radioactive precursor showed linear correlation with drug-induced lethality. The results of this test were also correlated well with in vivo regression of tumors of mice. This test appeared to be more reliable than other similar test of cell lethality in vitro. For utilization in clinical studies, a test plate was simplified and 25 human cancers were tested. The results demonstrated the generally good correlation between in vitro and in vivo tumor sensitivities.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004341:Drug Evaluation / Q000379:methods; D004351:Drug Resistance; D006801:Humans; D051379:Mice; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology*; D012588:Scintillation Counting; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D000970:Antineoplastic Agents; D014316:Tritium; D013936:Thymidine