[Synthesis and phosphorylation of histones and nonhistone proteins as affected by irradiation and serotonin in cycloheximide-synchronized hepatocytes].
Phosphorylation and synthesis of histones and nonhistone proteins were studied after the inhibition of translation by sublethal cycloheximide doses. Activation of the chromatin protein phosphorylation was noted: (1) at the stage of recovery and stimulation of the protein synthesis (18-24 h), and (2) at the stage of activation of the replicative DNA synthesis (30-60 h). Phosphorylation and synthesis of the chromatin proteins depended upon the individual or combined effect of X-radiation and serotonin. The authors discuss the possible role of the chromatin protein phosphorylation in the response of the nuclear apparatus to the effect of radiation and serotonin the latter being used as a radioprotective agent.
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis* / Q000528:radiation effects; D003513:Cycloheximide / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects; D006657:Histones / Q000096:biosynthesis* / Q000528:radiation effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D010766:Phosphorylation; D051381:Rats; D012701:Serotonin / Q000494:pharmacology*; D013997:Time Factors
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D012701:Serotonin; D003513:Cycloheximide
Sintez i fosforilirovanie gisotonov i negistonovykh belkov pod vliianiem oblucheniia i serotonina v sinkhronizirovannykh tsiklogeksimidom gepatotsitakh.