Differential response of avian red blood cell nucleosomes to heparin.
In avian erythrocyte chromatin, heparin interacts differentially with H1, H5 and the nucleosomal core histones. In non-erythroid cells, a partial extraction of H2A, H2B and H1 yields H3/H4/DNA complexes and particles of unchanged nucleosomal composition. The assay system for this heparin effect includes sucrose gradients, formaldehyde fixation and cesium chloride gradient centrifugation. A comparison of avian erythrocyte nucleosomes with chromatin subunits from precursor cells shows that H5 interferes with the heparin effect whereas a removal of H5 renders the core histones accessible to the polyanion.
D000740:Anemia / Q000097:blood; D000818:Animals; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D002645:Chickens; D004372:Ducks; D004912:Erythrocytes / Q000032:analysis*; D006493:Heparin / Q000494:pharmacology*; D006657:Histones / Q000097:blood* / Q000302:isolation & purification; D009707:Nucleosomes / Q000032:analysis* / Q000648:ultrastructure; D051381:Rats; D013950:Thymus Gland / Q000032:analysis
D006657:Histones; D009707:Nucleosomes; D006493:Heparin