Demonstration of T15 idiotype-positive effector and suppressor T cells for phosphorylcholine-specific delayed-type hypersensitivity response in CBA/N or (CBA/N X BALB/c)F1 male mice.
Subcutaneous immunization of CBA/N or (CBA/N X BALB/c)F1 (NBF1) male mice, which were defective in phosphorylcholine (PC)-specific humoral antibody response, with PC-conjugated syngeneic spleen cells induced a PC-specific delayed-type hypersensitivity (DTH) response, while intravenous administration of the same cells induced PC-specific suppressor T cells for the DTH response. Treatment of PC-specific effector or suppressor T cells for the DTH response induced in NBF1 male mice with anti-T15 idiotypic antibody inactivated effector or suppressor functions of these T cells implying that PC-specific T cells in NBF1 mice expressed T15 idiotypic determinants on their surface. Enrichment of PC-specific suppressor T cells was also shown by employing anti-T15 antibody-coated dishes.
D000818:Animals; D002448:Cell Adhesion; D002794:Choline / Q000031:analogs & derivatives*; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007130:Immunoglobulin Idiotypes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D010767:Phosphorylcholine / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D010132:p-Azobenzenearsonate / Q000276:immunology
D007130:Immunoglobulin Idiotypes; D010767:Phosphorylcholine; D010132:p-Azobenzenearsonate; D002794:Choline