Heterozygosis of phage 16-3 of Rhizobium meliloti: moderate level of mismatch repair or gene conversion.
Analysis of clear/turbid mottled (heterozygotic plaques) of Rhizobium meliloti temperate phage 16-3 indicates that the efficiency of repair at three sites (ti3, ti4, and ti5) in the C cistron is 2 to 20-fold less than that observed in E. coli phage lambda. In agreement with this conclusion, heterozygotic plaques were observed at similar frequency in crosses where point and small deletion mutants were combined, suggesting that in Rhizobium, DNA molecules with short single-stranded loops can escape from repair as efficiently as the simple mismatches.
D010582:Bacteriophage lambda / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics*; D003433:Crosses, Genetic; D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005785:Gene Conversion; D006579:Heterozygote; D012231:Rhizobium; D010948:Viral Plaque Assay
D004277:DNA, Single-Stranded