Rapid quantitative liquid chromatographic determination of caffeine levels in plasma after oral dosing.
A simple method is described for the rapid, quantitative analysis of caffeine in human plasma. Caffeine levels present in plasma following drug administration were determined by high-performance liquid chromatography with UV detection at 273 nm after plasma protein precipitation. Caffeine was detectable at levels as low as 0.1 micrograms/mL. Mean recoveries of 98% with a coefficient of variation of 3% were obtained for plasma standards, in which concentrations ranged from 0.1 to 8 micrograms/mL. Interassay variability of the slope of the standard curve had a coefficient of variation of 3%. Application of this method during human bioavailability studies is described.
D000284:Administration, Oral; D001682:Biological Availability; D002110:Caffeine / Q000008:administration & dosage / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D012727:Sex Characteristics; D013607:Tablets
D013607:Tablets; D002110:Caffeine