Purification and characterization of pancreatic colipase from the dogfish (Squalus acanthius).
Pure colipase from dogfish (Squalus acanthius) was obtained from an extract of pancreatic gland. It has a high isoelectric point (10.2) and the molecular weight was calculated to be 9108-9383. The N-terminal sequence was shown to be Gly-Leu-Phe-Leu-Asn-Leu-Ser-Ala-Gly-Glu-Leu-Cys-Val-Gly-Ser-Phe-Gln -Cys-Lys-Ser-Ser-Cys-Cys-Gln-Arg-Glu-Thr-Gly-Leu-Ser-Leu-Ala -Arg-Cys-Ala-. This sequence shows great homology with colipases from man, horse, pig and hen. There were indications of the existence of a proform of dogfish colipase. The propeptide was found to be Ala-Pro-Glu-Arg.
D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D003089:Colipases / Q000302:isolation & purification*; D004284:Dogfish; D006736:Horses; D006801:Humans; D007526:Isoelectric Point; D010179:Pancreas / Q000032:analysis*; D011506:Proteins / Q000302:isolation & purification*; D013552:Swine
D003089:Colipases; D011506:Proteins