Fluorescence probes in metastatic B16 melanoma membranes.
Fluorescence probe molecules, trans-parinaric acid and 1,6-diphenylhexatriene, were utilized to characterize the structure of plasma membranes, microsomes and mitochondria from B16 melanoma cells. High metastastic B16-F10 and low metastatic B16-F1 melanoma cell lines had markedly different membrane structures. The fluorescence polarization, fluorescence lifetime and limiting anisotropy of trans-parinaric acid were significantly lower (P less than 0.05) in all three membrane fractions of the B16-F1 cell line than in the corresponding membranes of the B16-F10 cell line. These data indicated less restriction to rotational motion in the solid lipid domains of B16-F1 cell membranes preferentially sensed by trans-parinaric acid. The limiting anisotropy of both trans-parinaric acid and 1,6-diphenyl-1,3,5-hexatriene was significantly lower in the outer monolayer than the inner monolayer of the plasma membrane of B16-F1 cells but not in B16-F10 cells. A breakpoint in Arrhenius plots of fluorescence near 30-34 degrees C indicated the presence of a phase separation that was assigned to the inner monolayer of the plasma membrane. However, no differences in this breakpoint temperature were noted between the B16-F1 and B16-F10 melanoma membranes. Thus, more fluid solid membrane domains and a distinct transbilayer fluidity difference were characteristic of plasma membranes from low metastatic B16-F1 melanoma cells in contrast to high metastatic B16-F10 melanoma cells.
D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D005456:Fluorescent Dyes; D007425:Intracellular Membranes / Q000502:physiology; D008545:Melanoma / Q000473:pathology / Q000503:physiopathology*; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000502:physiology; D051379:Mice; D008861:Microsomes / Q000502:physiology; D008928:Mitochondria / Q000502:physiology; D009362:Neoplasm Metastasis; D010743:Phospholipids / Q000502:physiology; D013050:Spectrometry, Fluorescence; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology
D005456:Fluorescent Dyes; D008563:Membrane Lipids; D010743:Phospholipids; D014302:Trinitrobenzenesulfonic Acid