Correlation between suppression of B cell anti-hapten response and generation of cytotoxic T cells induced by hapten-labelled syngeneic lymphocytes.
The correlation between suppression of B cell antibody response and generation of cytotoxic T cells induced by hapten (FITC) labelled syngeneic lymphocytes was studied. Primary in vitro sensitization with lymphocytes labelled with the hapten concentrations (FITC 0.5 and FITC 0.05) previously shown to induce specific B cell unresponsiveness generated cytotoxic cells that specifically lysed haptenated syngeneic blast cells. In vivo priming with either SC-FITC 0.5 or SC-FITC 0.05 could be demonstrated after in vitro restimulation with similarly haptenated cells. Thus, cells from primed mice gave a cytotoxic response already after 3 days in culture, whereas primary responses were only observed on day 5. Furthermore, immunization was evident as an increased specific lysis at low effector/target ratios. However, no direct cytotoxicity could be detected in spleen cells from immune animals. Comparison between kinetics of in vivo development of specific antibody suppression and induction of cytotoxic cells detectable after in vitro boosting revealed no correlation between the two phenomena.
D000818:Animals; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D006241:Haptens / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D013861:Thiocyanates / Q000276:immunology*
D006241:Haptens; D013861:Thiocyanates