Differential labelling of UDP-N-acetylglucosamine in Huntington's-chorea fibroblasts.
The hypothesis that there is impaired endogenous synthesis of glucosamine 6-phosphate in Huntington's-chorea fibroblasts was tested by double labelling matched pairs of fibroblasts in culture with carrier-free H3 32PO4 and [U-14C]glucosamine. The [32P]UDP-N-acetyl[14C]glucosamine and [14C]glucosamine 6-[32P]phosphate of the cellular soluble fraction was isolated by charcoal column and paper chromatography. There is no quantitative difference in 32P but a significant difference in 14C in these two sugars in a ratio of approx. 1.5 for Huntington's-chorea fibroblasts compared with normal fibroblasts.
D002250:Carbon Radioisotopes; D002460:Cell Line; D005347:Fibroblasts / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006595:Hexosamines / Q000378:metabolism; D006801:Humans; D006816:Huntington Disease / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D012867:Skin / Q000378:metabolism; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism*; D014539:Uridine Diphosphate Sugars / Q000378:metabolism*
D002250:Carbon Radioisotopes; D006595:Hexosamines; D009711:Nucleotides; D010761:Phosphorus Radioisotopes; D014539:Uridine Diphosphate Sugars; D014537:Uridine Diphosphate N-Acetylglucosamine; D005944:Glucosamine