A chondroitin sulphate proteoglycan from human cultured glial and glioma cells. Structural and functional properties.
A chondroitin sulphate proteoglycan capable of forming large aggregates with hyaluronic acid was identified in cultures of human glial and glioma cells. The glial- cell- and glioma-cell-derived products were mutually indistinguishable and had some basic properties in common with the analogous chondroitin sulphate proteoglycan of cartilage: hydrodynamic size, dependence on a minimal size of hyaluronic acid for recognition, stabilization of aggregates by link protein, and precipitability with antibodies raised against bovine cartilage chondroitin sulphate proteoglycan. However, they differed in some aspects: lower buoyant density, larger, but fewer, chondroitin sulphate side chains, presence of iduronic acid-containing repeating units, and absence (less than 1%) of keratan sulphate. Apparently the major difference between glial/glioma and cartilage chondroitin sulphate proteoglycans relates to the glycan rather than to the protein moiety of the molecule.
D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D002808:Chondroitin Lyases; D011508:Chondroitin Sulfate Proteoglycans / Q000276:immunology / Q000378:metabolism*; D002850:Chromatography, Gel; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D046911:Macromolecular Substances; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*
D011508:Chondroitin Sulfate Proteoglycans; D046911:Macromolecular Substances; D011509:Proteoglycans; D002808:Chondroitin Lyases