A high acceleration programmable centrifuge used in purification of myocardial and skeletal muscle myosins.
Protein purification can be improved by using high acceleration - deceleraton centrifugation. This study describes a high acceleration programmable centrifuge which reaches 5,000 x g in 3 sec and brakes from 5,000 to 0 x g in 4.3 sec. This study further describes the use of this centrifuge in myosin purification and thus demonstrates that protein purification can be improved by separating particles with a high acceleration - deceleration centrifuge for the following reasons: (1) biological and chemical equilibria are immediately terminated, (2) proteolysis is reduced, (3) working time is decreased, and (4) the native state of labile proteins are better preserved. Rapid acceleration and deceleration is advantageous in reducing centrifugation time for separation of particles because it decreases diffusion time of particles and non-desirable interactions.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002498:Centrifugation / Q000295:instrumentation*; D006224:Cricetinae; D004285:Dogs; D004586:Electrophoresis; D009132:Muscles / Q000032:analysis* / Q000201:enzymology; D009206:Myocardium / Q000032:analysis*; D009218:Myosins / Q000302:isolation & purification*; D011898:Ranidae
D000251:Adenosine Triphosphatases; D009218:Myosins