Efficiency of phage protein synthesis in lambda-infected E. coli minicells.
Phage lambda major head protein, the gene E product, has been identified among other phage proteins synthesized in lambda-infected Escherichia coli minicells, separated by SDS-acrylamide gel electrophoresis. On stained gels, the same protein has also been detected among total (bacterial and phage) proteins of lambda-infected minicells. The contribution of lambda proteins to the total protein content of lambda-infected minicells was found to be about 12% following 30 min lambda-infection. The inhibition of lambda early protein synthesis (shown by other authors in nucleate bacterial cells) practically does not occur in minicells; this may be the reason of the observed high efficiency of phage protein synthesis.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D010582:Bacteriophage lambda / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D008970:Molecular Weight; D009154:Mutation; D014764:Viral Proteins / Q000096:biosynthesis*
D001426:Bacterial Proteins; D014764:Viral Proteins