[Binding of gonadotropin-releasing hormone (Gn-RH) to pituitary membranes in male rats].
The binding of gonadotropin-releasing hormone (Gn-RH) to membranes of male rats pituitary cells was studied. It appeared that Gn-RH was bound to 3 classes of sites and that the concentration in high affinity binding sites was very low. Moreover there was negatively cooperative interaction among these high affinity binding sites leading to a rapid decrease in binding affinity and consequently unmeasurable displacement of labeled hormone with large amounts of radio-inert ligand. These facts are inconsistent with the possible estimation of Gn-RH by radioreceptor assay.
D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008861:Microsomes / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism*; D051381:Rats
D007987:Gonadotropin-Releasing Hormone
Etude de la liaison de la gonadotropin-releasing hormone (Gn-RH) aux membranes de l'hypophyse du rat mâle.