Histone acetylation and deacetylation of nuclei from sea urchin Hemicentrotus pulcherrimus embryos during early stages of development.
Nuclei were isolated from sea urchin (Hemicentrotus pulcherrimus) embryos during early stages of development, and the rates of histone acetylation and deacetylation were examined. Histone acetylation was measured in the presence of 6 mM sodium n-butyrate, a potent inhibitor of deacetylase, and in the absence of the inhibitor. The deacetylation activity was estimated from the difference between the rates with and without the inhibitor. The activities of the acetylation and the deacetylation, and the ratio of deacetylation to acetylation were unchanged during the stages between early blastula and gastrula. Autoradiographic analysis of acetylated histones showed that all of the internal histones of the nucleosomes were acetylated and that no apparent difference was found in the pattern of the acetylated fractions during the stages compared.
D000107:Acetylation; D000367:Age Factors; D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012617:Sea Urchins / Q000196:embryology*
D002087:Butyrates; D006657:Histones