Determination of IgE-containing immune complexes in human sera. Evaluation of polyethylene glycol precipitation of monomeric and complex IgE and of the detectability of IgE in the complexes.
We studied the polyethylene (PEG) precipitability of monomeric human IgE, and of human IgE artificially complexed with rabbit anti-human IgE. At conditions where precipitation of monomeric IgE did not occur, from 0.2 to 20% of the complexed IgE was precipitated. The PEG precipitability of the complexes was inversely related to the IgE/anti-IgE ratio used for preparation of the complexes. From 1.5 to 19.2% of the IgE in the redissolved precipitates could be detected by use of a two-site IgE immunoradiometric assay, the percentage being highest for complexes formed at equivalence. We conclude that exact quantitation of circulating IgE immune complexes (IC) probably is impossible by any PEG precipitation assay. However, the optimized assay was found to be useful for identification of IgE IC in sera with total IgE concentrations below 5,000 U/ml. IgE IC were found in 5/20 sera from patients with Felty's syndrome, in 5/39 sera from patients with extrinsic allergy and high levels of specific IgE, and in 1/17 sera from immunized wasp allergics. No IgE IC were found in 20 normal human sera.
D000936:Antigen-Antibody Complex / Q000032:analysis*; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D005069:Evaluation Studies as Topic; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology*; D011092:Polyethylene Glycols
D000936:Antigen-Antibody Complex; D007073:Immunoglobulin E; D011092:Polyethylene Glycols