An in vivo cholinergic influence on the retention of [3H]noradrenaline in regional brain synaptosomes.
Rats were intraventricularly (icv) injected with [3H]noradrenaline and the retention of the amine was determined in synaptosomes obtained from cerebral cortex, hypothalamus and brain stem. Previous icv administration of hemicholinium-3, effective enough to markedly decrease brain acetylcholine levels, increased the retention of synaptosomal [3H]noradrenaline in hypothalamus and cerebral cortex; this increased retention did not occur in the brain stem. The increased retention of [3H]noradrenaline, produced by hemicholinium-3, was reversed by a concomitant icv dose of choline, which in turn reversed the decrease of acetylcholine caused by hemicholinium-3. These results are interpreted as brain cholinergic activity having an influence on the turnover of noradrenaline in some brain regions.
D000109:Acetylcholine / Q000096:biosynthesis*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002794:Choline / Q000494:pharmacology; D006426:Hemicholinium 3 / Q000008:administration & dosage / Q000494:pharmacology*; D007276:Injections, Intraventricular; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000378:metabolism*; D013997:Time Factors; D014018:Tissue Distribution
D006426:Hemicholinium 3; D002794:Choline; D000109:Acetylcholine; D009638:Norepinephrine