Procedure for assay of tobramycin in serum containing moxalactam.
Moxalactam was examined as a substrate for 11 beta-lactamases. Hydrolysis was shown only with the beta-lactamase from Bacillus cereus, a nonpathogenic bacterium. Tobramycin was assayed in tobramycin-moxalactam mixtures by using this beta-lactamase.
D000900:Anti-Bacterial Agents / Q000097:blood*; D001409:Bacillus cereus / Q000201:enzymology; D001412:Bacillus subtilis / Q000201:enzymology; D001681:Biological Assay / Q000379:methods*; D002510:Cephalosporinase / Q000378:metabolism; D002511:Cephalosporins / Q000097:blood*; D002513:Cephamycins / Q000097:blood* / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D009070:Moxalactam; D014031:Tobramycin / Q000097:blood*; D015032:Zinc / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D002513:Cephamycins; D002510:Cephalosporinase; D015032:Zinc; D009070:Moxalactam; D014031:Tobramycin