A sensitive, continuous spectrophotometric method for assaying alpha-glycerophosphate dehydrogenase: activation by menadione.
By use of a more sensitive method than standard assays, it is demonstrated that alpha-glycerophosphate dehydrogenase activity can be monitored continuously in rat liver mitochondria; maximum activity was obtained by sonication in the presence of Triton X-100. Vitamin K3 (menadione) seems to enhance the activity of the enzyme. The assay in the presence of menadione is linear over a much greater mitochondrial concentration (up to 250 micrograms of protein in the reaction mixture).
D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D005993:Glycerolphosphate Dehydrogenase / Q000032:analysis* / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology; D017830:Octoxynol; D010619:Phenazines; D011092:Polyethylene Glycols; D051381:Rats; D011919:Rats, Inbred Strains; D013010:Sonication; D013053:Spectrophotometry / Q000379:methods; D013778:Tetrazolium Salts; D014812:Vitamin K / Q000494:pharmacology*
D010619:Phenazines; D013778:Tetrazolium Salts; C028122:5-ethylphenazine; D014812:Vitamin K; C013574:iodonitrotetrazolium; D011092:Polyethylene Glycols; D017830:Octoxynol; D005993:Glycerolphosphate Dehydrogenase