On-line computer analysis of chemiluminescent reactions, with application to a luminescent immunoassay for free cortisol in urine.
We interfaced a microcomputer on-line with a luminometer to acquire the light signal of chemiluminescent reactions from a photomultiplier and then compute significant parameters of light emission and kinetic "shape" indices. Using this system to study interferences from biological samples on the measurement of chemiluminescent reactions, we observed that such effects are usually associated with modifications of the shape of the light-emission kinetics. These results suggest that a simultaneous evaluation of the shape of a chemiluminescent reaction and the measurement of light emission can be combined to assess luminescent immunoassays as an internal control of the interferences in measurements of the chemiluminescent tracer. As an example of this approach, we developed and validated a luminescent immunoassay for free cortisol in diluted urine. Dextran-coated charcoal is used for bound-free separation.
D055598:Chemical Phenomena; D002621:Chemistry; D005069:Evaluation Studies as Topic; D006801:Humans; D006854:Hydrocortisone / Q000097:blood / Q000652:urine*; D007118:Immunoassay / Q000379:methods; D008163:Luminescent Measurements; D008838:Microcomputers; D011863:Radioimmunoassay
D006854:Hydrocortisone