5-Fluoro-2'-deoxyuridine incorporation in L1210 DNA.
We have employed cesium sulfate density gradient centrifugation to separate RNA and DNA of L1210 cells labeled with [3H]fluorodeoxyuridine. We have analyzed nucleotide and nucleoside digests of purified DNA from the [3H]fluorodeoxyuridine-labeled cells and demonstrate by reverse phase and anion exchange high pressure liquid chromatography the presence of tritium radioactivity co-migrating with fluorodeoxyuridine 5'-monophosphate or fluorodeoxyuridine. These observations demonstrate the internucleotide incorporation of fluorodeoxyuridine in DNA and suggest a new mechanism of action for this cytotoxic and mutagenic agent.
D000818:Animals; D004273:DNA, Neoplasm / Q000096:biosynthesis* / Q000302:isolation & purification; D005467:Floxuridine / Q000378:metabolism*; D007700:Kinetics; D007939:Leukemia L1210 / Q000378:metabolism*; D051379:Mice; D012334:RNA, Neoplasm / Q000302:isolation & purification
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D005467:Floxuridine