Characterization of rat spleen cell populations. II. Activation by antigens and allogeneic cells.
Spleen cells from BN rats were separated in a discontinuous density gradient. Cells from different fractions were shown to be functionally diverse. Light cells were highly responsive in MLR, generated little killer activity and enhanced syngeneic recipient's IgM and IgG production in response to SRBC. Dense cells were unable to respond in MLR and suppress Ab response. Maximum killer activity was recovered from medium density fractions which were probably mixtures of helper and suppressor cells.
D000818:Animals; D000941:Antigens / Q000276:immunology*; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D004912:Erythrocytes / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D051381:Rats; D011914:Rats, Inbred BN; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology / Q000637:transplantation; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014185:Transplantation, Isogeneic
D000941:Antigens