[Experimental models for the study of antiseptics].
Determination of the bactericidal concentration of antiseptics in vitro is now standardized, allowing interesting comparisons. Conversely, measurement of this activity in vivo in man is difficult since results are dependent upon skin sampling techniques and variations in bacterial growth from individual to individual and from one site to the next. Skin sampling can be standardized by using the hologenic Hairless mouse as an experimental model (skin needle biopsy and crushing). However, variations from one animal to the next remain a problem, even though results are very similar to those found in man. We therefore used monoxenic Hairless mice contaminated with S. aureus, S. epidermidis and P. aeruginosa. All experiments on these animals demonstrated consistency of bacterial cutaneous and digestive growth over time and smallness of variations in bacterial flora from animal to animal. We advocate use of this second model for standardization of evaluation of bactericidal activity of antiseptics.
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D004064:Digestive System / Q000382:microbiology; D005856:Germ-Free Life; D051379:Mice; D008812:Mice, Hairless; D012867:Skin / Q000382:microbiology*
D000891:Anti-Infective Agents, Local
Modèles expérimentaux d'étude des antiseptiques.