Determination of plasma levels of two aromatic retinoic acid analogues with antipsoriatic activity by high-performance liquid chromatography.
A method is described for the quantitative analysis in plasma of Ro 10-9359, an aromatic retinoic acid analogue, ethyl all-trans-9-(4-methoxy-2,3,6-trimethylphenyl)-3,7-dimethyl-2,4,6,8-nonatetraenoate and its major metabolite Ro 10-1670, all-trans-9-(4-methoxy-2,3,6-trimethylphenyl)-3,7-dimethyl-2,3,6,8-nonatetraenoic acid. The compounds are extracted from patient plasma with diethyl ether, separated on a reversed-phase column and detected and quantified by their UV absorption. The experimental error is below 9% in the concentration range 42-445 ng/ml. The detection limit is about 10 ng/ml. The method was applied to the analysis of plasma levels in healthy volunteers, receiving 75 mg orally.
D017255:Acitretin; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005050:Etretinate / Q000097:blood*; D006801:Humans; D007700:Kinetics; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000097:blood
D014212:Tretinoin; D005050:Etretinate; D017255:Acitretin