[Relation between glutathione and cytoplasmic protein sulfhydryl groups in the rat: a rapid procedure for analytical determination].
A simple rapid determination of glutathione (GSH) and cytoplasmic protein bound SH groups (PBSH), appropriate to study their relationship in tissues, in rat liver, kidney and testis was developed. Hepatic GSH and PBSH were measured after treatment with methyl iodide (400, 800 mg/kg, after 0.5 h), diethyl maleate (2 ml/kg, after 1h), carbon tetrachloride (1.2 ml/kg, after 3 h), phenobarbital (80 mg/kg for 3 days). Methyl iodide and diethyl maleate showed a decrease of GSH and PBSH; after treatment with phenobarbital an increase of GSH and PBSH was observed; no decrease of GSH and PBSH was found after carbon tetrachloride intoxication.
D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000032:analysis*; D005978:Glutathione / Q000032:analysis*; D006847:Hydrocarbons, Iodinated / Q000494:pharmacology; D008099:Liver / Q000032:analysis / Q000187:drug effects; D008298:Maleates / Q000494:pharmacology; D008722:Methods; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013438:Sulfhydryl Compounds / Q000032:analysis*
D003598:Cytoskeletal Proteins; D006847:Hydrocarbons, Iodinated; D008298:Maleates; D013438:Sulfhydryl Compounds; C014055:methyl iodide; C014476:diethyl maleate; D005978:Glutathione; D010634:Phenobarbital
Relazione tra glutatione e gruppi sulfidrilici proteici citoplasmatici nel ratto: rapida procedura di determinazione analitica.