Determination of zinc in erythrocytes, granulocytes and serum by flame atomic absorption spectrophotometry.
Zinc was determined in granulocytes, erythrocytes and serum by flame atomic absorption spectrophotometry. Using a Teflon sampling cup, the sample volume for a single granulocyte zinc determination was reduced to 100 microliter. Zinc in serum and erythrocytes was measured by aspirating the sample at a rate of 5 ml/min. The total volume of blood used was 23-28 ml. The intra-assay coefficient of variation of the method for granulocytes was 0.01 and of the method for serum and erythrocytes 0.018. The corresponding inter-assay coefficient of variation was 0.037 and 0.03, respectively. Recovery of zinc determined by the method of aspirating the small volume (100 microliter) was 103% and by the method of aspirating 5 ml/min 97%. The coefficient of correlation between the two methods was r = 0.9968.
D000328:Adult; D004912:Erythrocytes / Q000032:analysis*; D005260:Female; D006098:Granulocytes / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D013054:Spectrophotometry, Atomic / Q000379:methods; D015032:Zinc / Q000097:blood*
D015032:Zinc