Suppression of the late stages of mitogen-induced human B cell differentiation by FC gamma receptors (FC gamma R) released from polymorphonuclear neutrophils.
During short incubation in serum-free medium, polymorphonuclear neutrophils (PMN) release soluble material that can be characterized as receptors for Fc IgG (Fc gamma R) on the following evidence: it agglutinates erythrocyte-IgG antibody (EAG) complexes, it prevents the binding of EAG to EAG-rosette-forming cells, and it binds to EAG-rosette-forming cells after modulation of their Fc gamma R, allowing the formation of 'passive' rosettes. These Fc gamma R were isolated by affinity chromatography on sepharose 4B-IgG. This material was shown to interfere with the differentiation of peripheral blood B cells into Ig-secreting cells in cultures stimulated by pokeweed (PWM) or Nocardia opaca (NOC) extracts. The number of Ig-secreting cells determined by a reverse hemolytic plaque assay using protein A-coated sheep erythrocytes was decreased by addition of Fc gamma R over a wide range of dilutions. The number of Ig-containing cells was diminished in PWM-stimulated cultures, but not in cultures stimulated with NOC. Fc gamma R did not affect cell viability, nor did they interfere with plaque-forming cell assay. Fc gamma R was not suppressive when added before the 3rd day or after the 6th day of culture. The suppressor factor was shown to remain associated with Fc gamma R after elution at acidic pH; it was removed by absorption on Sepharose 4B-IgG, but not on pepsin-digested F(ab')2 fragments. The suppressive factor as well as the capacity to restore EAG rosette formation by modulated lymphocytes were destroyed by heating (56 degrees C, 30 min) or by freezing and thawing. Properties of Fc gamma R released from PMN in this system are similar to those of Fc gamma R released from unstimulated human peripheral blood lymphocytes (PBL) and to those of mouse Ig-binding factor produced by alloactivated T cells or T cell lines.
D000921:Antibody-Producing Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology*; D001666:Binding Sites, Antibody; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D006462:Hemolytic Plaque Technique; D006801:Humans; D007074:Immunoglobulin G; D007700:Kinetics; D008934:Mitogens / Q000494:pharmacology*; D009504:Neutrophils / Q000378:metabolism; D011043:Pokeweed Mitogens / Q000494:pharmacology; D011961:Receptors, Fc; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D013696:Temperature; D013997:Time Factors
D007074:Immunoglobulin G; D008934:Mitogens; D011043:Pokeweed Mitogens; D011961:Receptors, Fc