Studies on the male antifertility agent-gossypol acetic acid. II. Effect of gossypol acetic acid on the motility and ATPase activity of human spermatozoa.
A marked reduction in the motility of the human spermatozoa was observed when spermatozoa were incubated with 10 and 100 microgram of gossypol acetic acid for different time period at 37 degrees C; the motility was reduced from 83% to 4% after 210 minutes (P less than 0.001). A significant decrease in the activities of Ca++ and Mg++ activated ATPase in the spermatozoa was observed after gossypol treatment (P less than 0.005). Changes in the zinc++ concentration in the spermatozoa after gossypol treatment were, however, not significant. The present investigations suggest that inhibition of the sperm motility after gossypol treatment may be due to the inhibition of the ATPase activity in the spermatozoa.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D006072:Gossypol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D013081:Sperm Motility / Q000187:drug effects*; D013089:Spermatocidal Agents; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism; D015032:Zinc / Q000378:metabolism
D013089:Spermatocidal Agents; D000251:Adenosine Triphosphatases; D008274:Magnesium; D015032:Zinc; D006072:Gossypol; C028178:gossypol acetic acid; D002118:Calcium