Monoclonal antibodies against the LamB protein.
Eight independently derived monoclonal antibodies directed against the LamB protein were produced and characterized. These antibodies allowed the identification of 4 distinct topological and functional regions in the LamB molecule. Four monoclonal antibodies recognize determinants present at the cell surface. Two of these have their binding sites located in a region involved in maltose transport. One monoclonal antibody presumably binds to a determinant which is normally hidden in the membrane, and three monoclonal antibodies recognize a region of the protein which is in close association with the peptidoglycan sacculus.
D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000276:immunology* / Q000302:isolation & purification; D010582:Bacteriophage lambda; D002460:Cell Line; D004926:Escherichia coli / Q000276:immunology*; D018272:Porins; D011991:Receptors, Virus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D018272:Porins; D011991:Receptors, Virus; C016532:maltoporins