[Cell kinetics and radiosensitivity of goat lymphocytes stimulated by phytohemagglutinin].
The harlequin-staining method has been used to study the cell kinetic of goat peripheral blood lymphocytes stimulated by phytohemagglutinin and to assess their radiosensitivity. At 48 h, the standardized culture time employed for human lymphocytes, 71% of the goat lymphocytes are in first mitosis, 23% are in second mitosis and 5% in third. Irradiation with 200 rads X-rays induces an average of 24.5 dicentric chromosomes per hundred cells in first mitosis.
D000818:Animals; D002478:Cells, Cultured; D006041:Goats; D007621:Karyotyping; D007700:Kinetics; D008213:Lymphocyte Activation / Q000528:radiation effects; D008214:Lymphocytes / Q000502:physiology / Q000528:radiation effects*; D008938:Mitosis / Q000528:radiation effects*; D010835:Phytohemagglutinins
D010835:Phytohemagglutinins
Cinétique cellulaire et radiosensibilité des lymphocytes de chèvre stimulés par la phytohémagglutinine.