Penicillin-induced epileptic phenomena in the rabbit's neocortex I. The development of interictal spikes after epicortical application of penicillin.
In an attempt to elucidate the generation mechanisms underlying interictal spikes in the neocortex, the temporal development of spikes after the epicortical penicillin (PNC) application was studied. Field potentials (FP) were recorded simultaneously within the 6 neocortical layers with a multielectrode consisting of 16 contacts (10 X 10 micron 2) in a row at spacings of 150 micron. A one-dimensional current-source-density (CSD) analysis yielded the positions of current, sink and source densities, so that the different electrical events during a spike could be more accurately located within the different neocortical layers. After the epicortical application of PNC a typical succession of events, which underly the development of spikes, was observed. These events are similar in the visual and the motor cortex: immediately after the epicortical PNC application negative transients occur in the two uppermost cortical layers as well as within layers V to III. Due to the diffusion of the drug a characteristic succession of different processes takes place. Fully developed spikes show a typical configuration of sources and sinks, a moderate sink in layer V initiates a massive, double-peaked sink within layers II, III. This configuration of sinks suggests that some sort of triggering mechanism takes place. Since similar events are observed during interictal spikes in the visual and the motor cortex, neuronal structures common to both cortical areas are supposed to be responsible for the generation of PNC spikes.
D000818:Animals; D004553:Electric Conductivity; D004569:Electroencephalography; D004827:Epilepsy / Q000139:chemically induced*; D005260:Female; D008297:Male; D009044:Motor Cortex / Q000187:drug effects*; D010406:Penicillins / Q000494:pharmacology*; D011817:Rabbits; D014793:Visual Cortex / Q000187:drug effects*
D010406:Penicillins