Purification of the flavin-containing monooxygenase from mouse and pig liver microsomes.
The microsomal flavin-containing monooxygenase has been purified from mouse and pig liver utilizing Cibacron-Blue Sepharose, Procion-Red agarose, and 2'5'-ADP Sepharose. The enzymes had a final specific activity of 1200 and 954 nmol/min/mg protein from mouse and pig liver respectively. The enzyme from both mouse and pig liver displayed typical flavoprotein spectra and appeared homogeneous by denaturing polyacrylamide gel electrophoresis.
D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D005415:Flavins / Q000032:analysis; D005420:Flavoproteins / Q000302:isolation & purification; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D013045:Species Specificity; D013552:Swine
D005415:Flavins; D005420:Flavoproteins; D006899:Mixed Function Oxygenases