[Chalone or inhibine: control of progenitor spermatogonia in the adult rat by gonadal protein extracts].
Adult male rats received one injection (J0) of busulfan (10 mg/kg BW), which is specifically lethal for type A spermatogonia (Jackson and al., 1962). They were subsequently injected daily (J1 - J10) either with a proteinaceous extract of adult ram testis (10 mg of proteins/day) via the subcutaneous route, or with ram testicular fluid (1, 2 mg of protein/day), or with bovine serum albumin (1 mg/day) in order to analyse the resultant modification of the repopulation of seminiferous epithelium by the reserve stem cells (Clermont and Mauger, 1974). At the end of the treatments (J11), the number of type A spermatogonia per tubular cross section was significantly decreased in animals receiving testicular extract of fluid. The number of primary spermatocytes, from preleptotene up to zygotene stages were only diminished by testicular fluid supplementation. Ten days later (J20) the populations of primary spermatocytes, from preleptotene to pachytene stages derived from cells which were type A spermatogonia at the time of injections, were depleted. Type A spermatogonia were only decreased by testicular fluid treatment. Testicular fluid or extracts did not modify body weight nor the weights of other organs. A protein-like factor originating from the testis and present in the testicular fluid can thus modify the population of type A spermatogonia. Its action, however, is not specific for germ cells as Sertoli cells are affected by the administration of testicular extract in prepuberal rats (Hochereau-De-Reviers and Courot, 1978).
D000818:Animals; D002066:Busulfan / Q000494:pharmacology; D003864:Depression, Chemical; D006131:Growth Inhibitors; D008297:Male; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D012661:Semen / Q000032:analysis; D012670:Seminiferous Epithelium / Q000166:cytology*; D013093:Spermatogonia / Q000254:growth & development*; D013094:Spermatozoa / Q000254:growth & development*; D013737:Testis / Q000032:analysis / Q000166:cytology*; D013997:Time Factors; D014020:Tissue Extracts
D006131:Growth Inhibitors; D011506:Proteins; D014020:Tissue Extracts; D002066:Busulfan
Chalone ou inhibine: contrôle des spermatogonies souches du rat adulte par des extraits protéiques de gonades.