Isolation of avian reovirus as a possible etiologic agent of osteoporosis ("brittle bone disease"; "femoral head necrosis") in broiler chickens.
Avian reovirus was isolated from intestines of 3-to-7-day-old broiler chickens with enteritis from broiler houses where osteoporosis was a problem. The virus was purified in a cesium chloride gradient (buoyant density 1.37 gm/ml) and identified as a reovirus by electron microscopy. Specific-pathogen-free (SPF) chickens and commercial broiler chickens with anti-reovirus maternal antibodies inoculated at 1 day of age with the reovirus isolate developed lesions of femoral head fractures and/or osteoporosis; reovirus could be reisolated from the bone marrow and intestinal tracts of experimentally infected SPF birds. The reovirus isolate, although isolated from intestines, induced development fo tenosynovitis lesions in SPF and commercial broiler chickens.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002645:Chickens; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary; D005271:Femur Head Necrosis / Q000209:etiology / Q000662:veterinary*; D008854:Microscopy, Electron; D009500:Neutralization Tests; D010024:Osteoporosis / Q000209:etiology / Q000662:veterinary*; D011201:Poultry Diseases / Q000209:etiology*; D012087:Reoviridae / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D013047:Specific Pathogen-Free Organisms
D000914:Antibodies, Viral