High glucose-induced enzyme activity changes in cultured cell lines established from the kidneys of Chinese hamsters with aglycosuria or spontaneous glycosuria.
Chinese hamster kidney epithelial-like cells derived from highly inbred nondiabetic (AV) and diabetic (XA) genetic sublines were passaged in medium containing 100 or 400 mg/dl glucose. The effect of high medium glucose on the activities of 5 enzymes involved in glucose metabolism was followed and significant glucose-dependent difference was observed. The effects, however, were opposite in cells derived from AV and XA sublines.
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D003920:Diabetes Mellitus / Q000201:enzymology; D005947:Glucose / Q000494:pharmacology*; D005964:Glucosyltransferases / Q000378:metabolism; D006029:Glycosuria / Q000201:enzymology*; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008297:Male; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism
D003094:Collagen; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005964:Glucosyltransferases; C023247:UDP glucose-collagen glucosyltransferase; D011770:Pyruvate Kinase; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D005947:Glucose