Design and operation of a completely automated Beckman microsequencer.
A unique, efficient, and inexpensive system has been designed and built for the automatic conversion of anilinothiazolinone derivatives extracted from a Beckman spinning-cup sequencer with subsequent on-line high-pressure liquid chromatography separation of the phenylthiohydantoin derivatives. The Auto Converter-Auto Sampler system is controlled by a tape programmer or microprocessor and operates by transfer of the sample from the conversion vial into an HPLC injection loop by nitrogen pressure. Incorporation of a minor programming change on the sequencer allows the introduction of nitrogen vapor into the spinning cup during phenylisothiocyanate coupling. These modifications have resulted in a completely automated subnanomole protein sequencer.
D000595:Amino Acid Sequence; D001322:Autoanalysis / Q000295:instrumentation*; D002851:Chromatography, High Pressure Liquid; D008832:Microchemistry; D009584:Nitrogen; D010455:Peptides / Q000032:analysis; D010669:Phenylthiohydantoin / Q000031:analogs & derivatives / Q000032:analysis; D011506:Proteins / Q000032:analysis
D010455:Peptides; D011506:Proteins; D010669:Phenylthiohydantoin; D009584:Nitrogen