Cytosolic gangliosides of rat brain: their fractionation into protein-bound complexes of different ganglioside compositions.
The sialic acid moiety of rat brain cytosolic gangliosides was radiolabeled by intracranial injection of N-(3H)acetylmannosamine. Upon ammonium sulphate fractionation, Sepharose 6B gel filtration, and hydroxylapatite-cellulose chromatography, ganglioside-bound radioactivity of brain cytosolic extract followed the behavior of protein and not that of purified gangliosides. This indicates that cytosolic gangliosides occur as ganglioside-protein complexes. By application of hydroxylapatite-cellulose column chromatography, fractions were obtained having different ganglioside composition. In particular, one fraction contained GM1, one GD1a, and one GT1b with a ganglioside homogeneity better than 95% in each fraction. This indicates the occurrence in brain cytosol of a GM1-protein complex, a GD1a-protein complex, and a GT1b-protein complex.
D000818:Animals; D001923:Brain Chemistry; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D003600:Cytosol / Q000032:analysis; D005732:Gangliosides / Q000032:analysis* / Q000302:isolation & purification; D009419:Nerve Tissue Proteins; D051381:Rats; D011919:Rats, Inbred Strains
D005732:Gangliosides; D009419:Nerve Tissue Proteins