title: Cellular mode of serotonin action on Cl- transport in the rabbit corneal epithelium.
abstract: The present work examines serotonin-induced changes in cell potential difference and barrier resistances in the corneal epithelium in vitro using voltage-measuring microelectrodes and related techniques. Component resistances were determined using voltage and resistance profiles of the epithelium before and during the serotonin response. Serotonin, added to the stromal side of the cornea in the presence of nialamide, markedly reduced transcorneal and apical membrane resistances, while basal barrier resistance increased slightly and shunt resistance was unchanged. The marked drop in apical membrane resistance after serotonin treatment reflects an increase in apical membrane chloride permeability, inasmuch as the serotonin-stimulated short-circuit current is indistinguishable from the increase in net chloride flux. Prolonged (more than 1 h) exposure of corneas to serotonin markedly depolarized the epithelial cells and reduced the voltage divider ratio from 12.3 +/- 2.1 to 1.5 +/- 0.5, while not significantly affecting the stimulated short-circuit current. These later effects suggest changes in epithelial ion distribution during long periods of stimulation by serotonin.
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000502:physiology; D002463:Cell Membrane Permeability; D002712:Chlorides / Q000378:metabolism*; D003315:Cornea / Q000502:physiology*; D004553:Electric Conductivity; D004848:Epithelium / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D011817:Rabbits; D012701:Serotonin / Q000494:pharmacology*
chemical_list: D002712:Chlorides; D012701:Serotonin