[Repaired and unrepaired DNA damage induced by x-irradiation of nonproliferating rat hepatocytes].
An effective system of repair of double-strand DNA breaks was found in the stock culture of rat hepatocytes by the method of elastoviscosimetry. The inhibitors of DNA resynthesis, hydroxyurea and arabinosyl cytosine, which transformed single-strand breaks into double-strand ones were used to estimate the level of spontaneous affection of the hepatocyte genome. With a reference to this level the authors have demonstrated that after a dose of 2 Gy and 6-24-hour repair, the additional number of lesions was registered. These lesions were shown to be incised by nucleases similar to those involved into the conventional repair process.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000528:radiation effects*; D004260:DNA Repair / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000528:radiation effects; D004278:DNA, Superhelical / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D008099:Liver / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D051381:Rats; D013997:Time Factors
D004277:DNA, Single-Stranded; D004278:DNA, Superhelical; D004247:DNA
Repariruemye i nerepariruemye povrezhdeniia DNK, voznikaiushchie pri rentgenovskom obluchenii neproliferiruiushchikh gepatotsitov krys.