title: Species differences in monoamine oxidase-A and -B as revealed by sensitivity to trypsin.
abstract: Monoamine oxidase-A and -B of brain and peripheral tissues of human, dog, rat, and rabbit have been characterized with respect to kinetic parameters and response to limited trypsin treatment. MAO-A in all extracts was inactivated by trypsin to an extent that was species dependent, but not related to the nature of the tissue, to MAO-A/B ratios, or to kinetic parameters. MAO-B activity of human, dog, and rat was unaffected by trypsin under the conditions employed, but rabbit MAO-B was sensitive. Inactivation patterns obtained with the trypsin treatment described in this study provide additional evidence that structures of both MAO-A and MAO-B show species-specific variations.
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology; D004285:Dogs; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008297:Male; D008995:Monoamine Oxidase / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology; D010627:Phenethylamines / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism; D013045:Species Specificity; D013997:Time Factors; D014357:Trypsin / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D010627:Phenethylamines; C029261:phenethylamine; D012701:Serotonin; D008995:Monoamine Oxidase; D014357:Trypsin