Preparation of an immunotoxin for Acanthamoeba castellanii.
Acanthamoeba castellanii (Neff) is a small free-living amoeba which is closely related to pathogenic organisms that produce almost invariably fatal human infections (1). The f(ab)' fragment of a monoclonal antibody (designated A9) specifically reactive to the A. castellanii cell surface, was covalently linked to the A chain of diphtheria toxin. This immunotoxin inhibited cell division completely, whereas nonspecific mouse IgG or nonspecific f(ab)' derivatized with diphtheria toxin A chain (DTA), A chain alone, or unlinked A chain and A9-f(ab)' in combination, had no effect on cell division.
D000656:Amoeba / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D002455:Cell Division / Q000187:drug effects; D004167:Diphtheria Toxin / Q000276:immunology; D007140:Immunoglobulin Fab Fragments; D010446:Peptide Fragments / Q000276:immunology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D004167:Diphtheria Toxin; D007140:Immunoglobulin Fab Fragments; D010446:Peptide Fragments; C029795:diphtheria toxin fragment A