title: Mitogen-induced human IgG subclass expression.
abstract: To investigate human isotype expression among lymphocyte populations, we have studied IgG subclass production by splenocytes, tonsil cells, and peripheral blood lymphocytes (PBL) after stimulation with a panel of nine mitogens. Response magnitudes varied with tissues: all mitogens produced the strongest responses with splenocytes and the smallest with PBL. In addition, the IgG subclass maximally stimulated by a particular mitogen also depended on the tissue studied. For example, LPS mainly stimulated IgG2 in PBL and IgG1 in spleen. Interestingly, the response patterns seen in splenocytes suggest a large and coordinate expression of IgG1 and IgG3 subclasses. Implications of these findings are discussed with respect to immunoglobulin gene organization and human disease states.
mesh_terms: D000918:Antibody Specificity; D002648:Child; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000145:classification*; D007075:Immunoglobulin M / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D008934:Mitogens / Q000494:pharmacology*; D014066:Palatine Tonsil / Q000166:cytology; D013154:Spleen / Q000166:cytology
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008934:Mitogens