[Activation energy of enzymatic hydrolysis of different substrates of alpha-chymotrypsin].
Analysis of the experimental data for the series of alpha-chymotrypsin-catalyzed reactions of ester hydrolysis was carried out on the basis of modern elementary act theory of chemical processes. It was concluded that activation energy of considered reactions is attributed by repulsion potential for nucleophilic particle and carbonyl centre. It was supposed that the reason for abrupt changes of activation energy accompanying small enzyme-substrate complex structure shifts lies in the corresponding changes of carbonyl centre polarizability governed by its electrophilic environment.
D002918:Chymotrypsin / Q000378:metabolism*; D004579:Electron Transport; D004789:Enzyme Activation; D006868:Hydrolysis; D007700:Kinetics; D013379:Substrate Specificity
D002918:Chymotrypsin
Energiia aktivatsii fermentativnogo gidroliza razlichnykh substratov alpha-khimotripsina.