A granulopoiesis inhibitor partially purified from large-scale serum-free cultures of porcine leukocytes.
A method is described for the partial purification of a granulomonopoiesis inhibitor derived from 200 l-batches equivalent to 1 kg or 2 X 10(12) leukocytes of porcine blood. Serum-free conditioned media were concentrated and separated via ammonium sulfate precipitation, acetone partitioning, ultrafiltration and gel chromatography. The active substance revealed a molecular mass of 500-700 Da and was not thiol-dependent, thus contrasting it with previously-reported granulopoiesis inhibitors. Inhibition of colony formation in an in vitro myeloid stem cell assay using various colony-stimulating factors showed that mature granulocytes and monocytes were equally affected. Apparent lack of cytotoxicity was suggested using a new reversibility test.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D004355:Drug Stability; D006098:Granulocytes / Q000502:physiology*; D006410:Hematopoiesis; D006801:Humans; D007962:Leukocytes / Q000502:physiology*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D051379:Mice; D013552:Swine
D003470:Culture Media