Phenotype of cytotoxic effector cells infiltrating a transplanted, chemically induced rat sarcoma.
Previous work from this laboratory indicated that methylcholanthrene-induced sarcomas in the rat are infiltrated by cells with the cytotoxic properties of natural killer (NK) cells. Using a combination of velocity sedimentation and analysis and separation in the fluorescence-activated cell sorter, it has been possible to isolate and characterize these putative NK cells. The present results confirm that cytotoxicity is restricted to NK-sensitive targets (syngeneic, allogeneic and xenogeneic) and no evidence was found for cytotoxic T lymphocytes (CTL). In addition, effector cells have the morphology of large granular lymphocytes, and the pattern of expression of five monoclonal antibody-defined surface markers is identical with that of normal splenic NK cells.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D003602:Cytotoxicity, Immunologic; D005260:Female; D005354:Fibrosarcoma / Q000139:chemically induced / Q000276:immunology*; D005434:Flow Cytometry; D007694:Killer Cells, Natural / Q000276:immunology*; D008748:Methylcholanthrene; D009368:Neoplasm Transplantation; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000276:immunology
D000954:Antigens, Surface; D008748:Methylcholanthrene