Uptake and esterification of exogenous cholesterol by low-density-lipoprotein-receptor-negative human fibroblasts in culture.
The incorporation and metabolism of both vesicle- and LDL (low-density lipoprotein)- derived [3H]cholesterol by LDL-receptor-negative fibroblasts were studied. Independent of the cholesterol source, free [3H]cholesterol was readily incorporated into the cells and was available for esterification. 7-Oxocholesterol stimulated both [3H]cholesterol incorporation, by increasing the exchange rate, and the subsequent esterification of it irrespective of the source of exogenous [3H]cholesterol. The 7-oxocholesterol-stimulated esterification of exogenously derived LDL free [3H]cholesterol was progesterone-sensitive and energy-requiring.
D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D004951:Esterification; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000378:metabolism*; D007653:Ketocholesterols / Q000494:pharmacology; D011973:Receptors, LDL / Q000378:metabolism*
D007653:Ketocholesterols; D011973:Receptors, LDL; D002784:Cholesterol; C003001:7-ketocholesterol