Validation and use of centrifugal ultrafiltration-dialysis in the measurement of percent free oestradiol in serum.
The validity of the centrifugal ultrafiltration-dialysis method in the measurement of the percentage of oestradiol and testosterone which is not bound to protein (free) in human serum was assessed. In an initial validation, the values of % free steroid obtained for oestradiol and testosterone in protein-free ultrafiltrates of human serum were higher than expected. Filter paper discs used in the system were shown to adsorb [3H]oestradiol from protein-free solution. Their replacement with glass-fibre discs, which did not adsorb oestradiol, decreased but did not eliminate the discrepancy between observed and expected results for % free oestradiol in ultrafiltrates. The use of polyethylene rather than polypropylene vials then reduced the discrepancy to insignificant levels. The estimates for % free oestradiol in serum were reduced by these modifications.
D002499:Centrifugation, Density Gradient; D003956:Dialysis; D004958:Estradiol / Q000097:blood*; D005260:Female; D006801:Humans; D013739:Testosterone / Q000097:blood; D014462:Ultrafiltration
D013739:Testosterone; D004958:Estradiol