Simple, rapid, and quantitative release of periplasmic proteins by chloroform.
We introduce a method by which periplasmic proteins can be released rapidly, simply, and quantitatively by treating cells with chloroform. All the amino acid-binding proteins tested maintained their activity during chloroform treatment. This method makes practical the analysis of the periplasmic protein complement of a large number of strains.
D001426:Bacterial Proteins / Q000302:isolation & purification*; D002725:Chloroform; D004591:Electrophoresis, Polyacrylamide Gel; D006090:Gram-Negative Bacteria / Q000032:analysis*; D008970:Molecular Weight; D009154:Mutation; D009994:Osmolar Concentration; D013045:Species Specificity
D001426:Bacterial Proteins; D002725:Chloroform