A semi-integrated method for the determination of enzyme kinetic parameters and graphical representation of the Michaelis-Menten equation.
A semi-integrated method for the determination of the enzyme kinetics parameters (Km and V) and graphical representation of the Michaelis-Menten equation is proposed as a variation of determination of initial reaction rate (v) as a function of initial substrate concentration ([S]0). The method is based on the determination of the time required to exhaust half of the initial substrate concentration as a function of the initial substrate concentration. The advantages and limitations of this method are discussed.
D004791:Enzyme Inhibitors; D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D008956:Models, Chemical; D013379:Substrate Specificity
D004791:Enzyme Inhibitors; D004798:Enzymes