Electron-capture capillary gas chromatographic determination of phenylpropanolamine in human plasma following derivatization with trifluoroacetic anhydride.
A capillary gas chromatographic analysis of phenylpropanolamine in human plasma, following extraction and derivatization with trifluoroacetic anhydride, is presented. Using an electron-capture detector, the method was sensitive enough to quantitate as little as 1 ng of drug/mL of plasma. The coefficient of variation from 5-262 ng/mL varied between 5.6 and 1.6%, respectively. Plasma concentration data following one 25-mg dose of phenylpropanolamine hydrochloride in four healthy volunteers illustrates the suitability of this analytical method for monitoring plasma levels after oral administration of a typical dosage form.
D000089:Acetic Anhydrides; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas / Q000379:methods; D005463:Fluoroacetates; D006801:Humans; D007700:Kinetics; D010665:Phenylpropanolamine / Q000097:blood*; D014269:Trifluoroacetic Acid
D000089:Acetic Anhydrides; D005463:Fluoroacetates; D010665:Phenylpropanolamine; C017958:trifluoroacetic anhydride; D014269:Trifluoroacetic Acid