Fluorescence determination of microconcentrations of chlorambucil after photoactivation.
A fluorescence assay is described which measures the alkylating activity of chlorambucil or its isocyanate derivative after photoactivation in the presence of dimethyl sulfoxide. This assay has a lower limit of sensitivity of 100 ng/mL and RSD of less than 10% for chlorambucil. The method requires less than 5 micrograms of alkylating agent and the fluorophore produced is stable for at least 24 h.
D002699:Chlorambucil / Q000032:analysis*; D005453:Fluorescence; D010777:Photochemistry; D012016:Reference Values; D014466:Ultraviolet Rays
D002699:Chlorambucil