[Thrombocyte adhesion and aggregation on surfaces coated with human type-I, -III, -IV and -V collagens].
Human collagens of type I, III, IV, and V (CI, CIII, CIV, and CV) can be localized in different anatomic structures of the vessel wall. To investigate the role of vascular collagenous components in mural thrombus formation, the authors studied platelet adhesion to the wells of Falcon culture plates coated with: a) monomeric CI, CIII, CIV, and CV; b) fibrillar CI and CIII, and c) amorphous CIV and CV. On monomeric and amorphous CV, only initial attachment takes place, i.e. platelets bind to the surface without subsequent spreading. Platelet adhesion on monomeric and amorphous CIV proceeds more actively: the total level of adhesion is substantially higher than on CV, with up to 75% of adherent platelets spread out and single unspread platelets from suspension attached to the upper surface of spread platelets. On monomeric and fibrillar CI/CIII, formation of large multi-layer (thrombi-like) aggregates, with spread platelets at the basis, takes place along with processes characteristic for adhesion on CIV/CV. On the contrary, only fibrillar but not monomeric CI and CIII induce platelet aggregation in suspension. The data suggest that the ability of CI and CIII to induce platelet aggregation is fully conditioned by the genetic type of collagen and requires a simultaneous multivalent platelet-collagen interaction, which can be achieved by surface immobilization of collagen or formation of fibrillar structures in suspension.
D001792:Blood Platelets / Q000187:drug effects / Q000648:ultrastructure; D003094:Collagen / Q000494:pharmacology*; D005780:Gelatin / Q000494:pharmacology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010047:Ovalbumin / Q000494:pharmacology; D010973:Platelet Adhesiveness / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects*; D013499:Surface Properties
D005780:Gelatin; D010047:Ovalbumin; D003094:Collagen
Adgeziia i agregatsiia trombotsitov na poverkhnostiakh, pokrytykh kollagenami cheloveka I, III, IV i V tipov.