Methylglyoxal inhibits the translation of natural and chemically decapped mRNAs.
Methylglyoxal was a weak inhibitor of translation in the reticulocyte-lysate cell-free system and it did not display cap-dependent inhibition. A similar inhibition was obtained in a wheat-germ cell-free system that displayed extensive cap-dependent inhibition with the cap analogue 7-methylguanosine phosphate. These results show that the chemical reaction of methylglyoxal with 7-methylguanosine is not the mechanism for the inhibition of protein synthesis by methylglyoxal and that methylglyoxal is a weak general inhibitor of translation.
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D002474:Cell-Free System; D005260:Female; D005914:Globins / Q000235:genetics; D006151:Guanosine / Q000031:analogs & derivatives / Q000494:pharmacology; D008321:Mammary Glands, Animal / Q000378:metabolism; D010944:Plants / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects*; D011765:Pyruvaldehyde / Q000494:pharmacology*; D012315:RNA Caps / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D012156:Reticulocytes / Q000378:metabolism; D014908:Triticum / Q000378:metabolism
D000447:Aldehydes; D012315:RNA Caps; D012333:RNA, Messenger; D006151:Guanosine; C016578:7-methylguanosine; D011765:Pyruvaldehyde; D005914:Globins