The protein binding of timegadine determined by equilibrium dialysis.
The protein binding of timegadine to albumin, serum, plasma and plasma enriched with the acute phase reactants alpha 1-acid glycoprotein, alpha 1-anti-trypsin and C-reactive protein was determined by equilibrium dialysis. The effects of other analgesic and anti-inflammatories (indomethacin, ketoprofen, paracetamol and sodium salicylate) and other basic drugs (disopyramide, lignocaine, propranolol and quinidine) on the binding of timegadine were also determined. Timegadine binding was concentration-dependent up to 0.5 micrograms/ml, but independent above this level up to 10.0 micrograms/ml, the mean and standard error being 93.8 +/- 0.5%. Albumin accounted for only 32.4% of timegadine bound to plasma. Plasma enrichment with the acute phase reactants led to significant increases in timegadine binding. Simultaneous dialysis with other drugs caused significant decreases in timegadine binding.
D000893:Anti-Inflammatory Agents / Q000378:metabolism*; D000925:Anticoagulants / Q000494:pharmacology; D001798:Blood Proteins / Q000378:metabolism*; D003956:Dialysis; D006146:Guanidines / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D011485:Protein Binding; D012709:Serum Albumin / Q000032:analysis
D000893:Anti-Inflammatory Agents; D000925:Anticoagulants; D001798:Blood Proteins; D006146:Guanidines; D012709:Serum Albumin; C028998:timegadine