Photoprotective role of epidermal melanin granules against ultraviolet damage and DNA repair in guinea pig skin.
We previously developed a quantitative autoradiographic technique with special forceps for measuring unscheduled DNA synthesis (UDS) in mouse skin after treatment with ultraviolet light in vivo. By this method, we investigated the relationship between the protective role of melanin and UV-induced DNA repair in black-and-white guinea pigs. Flat areas containing a sharp border between pigmented and unpigmented skin were selected. The skin of the selected areas was shaved and irradiated with short-wave UV (254 nm) or UV-AB (270 to 440 nm, emission peak at 312 nm) at various doses. Immediately after irradiation, the skin was clamped off with forceps, and an isotonic aqueous solution of [methyl-3H]thymidine was injected s.c. into the clamped off portion. UDS was clearly demonstrated as silver grains in this portion of the skin after irradiation with 254 nm UV or UV-AB. Errors due to individual differences were avoided by comparing the intensities of UDS in basal cells from pigmented skin and unpigmented skin of the same animals. Unexpectedly, in groups of animals treated with 254 nm UV or UV-AB, no difference in UDS in pigmented and unpigmented skin was seen at any UV dose. These results suggested that epidermal melanin granules do not significantly protect DNA of basal cells against 254 nm UV or UV-AB irradiation. Results of a study on the effect of the wavelength of irradiation on the UDS response of albino guinea pigs are also reported.
D000818:Animals; D001345:Autoradiography; D003594:Cytoplasmic Granules / Q000502:physiology / Q000528:radiation effects*; D004260:DNA Repair; D004261:DNA Replication / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D006168:Guinea Pigs; D008543:Melanins / Q000502:physiology*; D011837:Radiation-Protective Agents; D012867:Skin / Q000528:radiation effects*; D012879:Skin Physiological Phenomena; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014466:Ultraviolet Rays
D008543:Melanins; D011837:Radiation-Protective Agents; D014316:Tritium; D013936:Thymidine