title: Simple method for isolation of 4-deoxynivalenol from rice inoculated with Fusarium graminearum.
abstract: A new method for preparative isolation of 4-deoxynivalenol (DON) is presented. This method avoids the loss of material during purification on silica gel by column chromatography. DON and 3-acetyldeoxynivalenol in crude extracts of rice inoculated with Fusarium graminearum were converted to triacetyldeoxynivalenol; the acetylated product was easier to purify by silica gel chromatography than DON is. After hydrolysis and further purification on a charcoal-alumina column, the 71% pure DON was recovered in yields as high as 450 mg of DON per kg of rice. Subsequent separation on a Sephadex LH20 column yielded DON that was greater than 90% pure.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005670:Fusarium / Q000378:metabolism*; D012275:Oryza / Q000032:analysis*; D012717:Sesquiterpenes / Q000302:isolation & purification*; D014255:Trichothecenes / Q000302:isolation & purification*
chemical_list: D012717:Sesquiterpenes; D014255:Trichothecenes; C007262:deoxynivalenol