The tumor-cell-releasing machine.
We describe the construction of a machine for the injection of 10- to 100-microliter volumes of viable cells in suspension. The cells are maintained in a tissue culture environment and can be injected according to a preprogrammed schedule. Preliminary results show that the tumor incidence and metastasis pattern of TA3Ha cells injected intravenously into mice are the same if the cells are injected in a single dose, or administered in 20 fractions during 3 h.
D000818:Animals; D002470:Cell Survival; D007275:Injections, Intravenous / Q000295:instrumentation*; D051379:Mice; D008805:Mice, Inbred A; D009368:Neoplasm Transplantation / Q000295:instrumentation / Q000379:methods*; D009360:Neoplastic Cells, Circulating; D013997:Time Factors