title: Influence of starvation on sucrase regulation by dietary sucrose in the rat.
abstract: We have studied the action of sucrose on jejunal sucrase activity. Rats (175 g) were first starved or fed a digestible carbohydrate-free diet for 60 h and then fed a high sucrose diet for varying times up to 84 h. 1) Rats starved for 60 h showed mucosal atrophy with a decrease in protein content/10 cm (18.00 +/- 1.4 versus 40.1 +/- 3 mg (controls p less than 0.001) and in villus height (357 +/- 18 versus 526 +/- 5 microns, p less than 0.001) which was fully repaired only after 60 h on the sucrose diet (528 +/- 11 microns). Rats on digestible carbohydrate-free diet showed no mucosal atrophy. 2) Starved rats had a delayed (60 h) sucrase activity response to sucrose (53 +/- 7 versus 122 +/- 4 microns/mg protein, p less than 0.001). Maximum activity was obtained after 12 h on sucrose diet in rats maintained on the carbohydrate-free diet: 38 +/- 1 versus 108 +/- 2.3 microns/mg protein, p less than 0.001. 3) Villus and crypt cell analysis after starvation and 12 h on a high sucrose diet localized the increase in sucrase activity to the villus-crypt junction. No change occurred in the upper villus. The increase was complete all along the villus by 36 h. In contrast, after the carbohydrate-free diet, sucrase activity increased maximally at all levels of the villus by 12 h on the high sucrose diet.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D004040:Dietary Carbohydrates / Q000494:pharmacology; D007583:Jejunum / Q000201:enzymology* / Q000473:pathology; D007700:Kinetics; D008297:Male; D051381:Rats; D013217:Starvation / Q000201:enzymology* / Q000473:pathology; D013393:Sucrase / Q000378:metabolism*; D013395:Sucrose / Q000494:pharmacology*; D014018:Tissue Distribution
chemical_list: D004040:Dietary Carbohydrates; D013395:Sucrose; D013393:Sucrase