title: Studies on the enhancement of human eosinophil function by mononuclear cell products in vitro.
abstract: Previous studies have shown that human mononuclear cells produce during culture an activity that enhances the functional properties of mature eosinophils, including their helminthotoxic potential (Veith & Butterworth, 1983). We have now found that the enhancing activity in mononuclear cell culture supernatants (MCS) is stable to dialysis for 24 h at pH 2 and sensitive to trypsin (0.1 mg/ml). It has a principal pI of 4.4 and possible secondary peaks with pI values of 5.0 and 5.7. The effect of MCS on eosinophils appears to be a general early stimulation of several functions. In comparison with eosinophils incubated in medium only, the proportion of eosinophils with Fc receptors is increased after incubation with MCS for 3.5 h (39 +/- 11% vs 16 +/- 3%) and 7 h (28 +/- 4% vs 17 +/- 3%). The temperature-dependent phase of eosinophil adherence to antibody coated schistosomula is enhanced after 3-4 h. The uptake of deoxyglucose is enhanced after a 1 h pre-incubation with MCS (24 +/- 1 X 10(3) d/min), compared with that of controls (15 +/- 2 X 10(3) d/min). Whether these effects are due to one mediator in MCS awaits further investigation.
mesh_terms: D002448:Cell Adhesion; D002474:Cell-Free System; D002478:Cells, Cultured; D003847:Deoxyglucose / Q000378:metabolism; D004804:Eosinophils / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D009000:Monocytes / Q000276:immunology*; D015846:Monokines; D011506:Proteins / Q000276:immunology*; D011961:Receptors, Fc / Q000032:analysis; D012550:Schistosoma mansoni / Q000276:immunology; D013696:Temperature; D014357:Trypsin
chemical_list: D015846:Monokines; D011506:Proteins; D011961:Receptors, Fc; D003847:Deoxyglucose; D014357:Trypsin