Synaptosomal phospholipase D: potential role in providing choline for acetylcholine synthesis.
The synaptic membrane (SM) prepared from rat brain synaptosomes displayed the highest activity of phospholipase D of mammalian tissue thus far observed. In common with the previously described latency of the microsomal enzyme, the SM phospholipase D (PLD) activity is barely detectable in the absence of 4 mM sodium oleate. Incubation of synaptosomes in the presence of choline labelled lecithin, sodium oleate and acetyl CoA resulted in the formation of a product co-chromatographing with acetylcholine. The potential role of SM PLD liberating choline from lecithin into the synaptic cleft is discussed in relationship to acetylcholine formation.
D000109:Acetylcholine / Q000096:biosynthesis*; D000818:Animals; D001921:Brain / Q000201:enzymology; D002794:Choline / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D010712:Phosphatidic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013570:Synaptic Membranes / Q000201:enzymology; D013574:Synaptosomes / Q000201:enzymology*
D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010740:Phospholipases; D010739:Phospholipase D; D002794:Choline; D000109:Acetylcholine