title: PTH release stimulated by high extracellular potassium is associated with a decrease in cytosolic calcium in bovine parathyroid cells.
abstract: We employed the calcium (Ca++)-sensitive, intracellular dye QUIN-2 to examine the role of cytosolic Ca++ in the stimulation of PTH release by high extracellular potassium (K+) concentrations. Addition of 55 mM KC1 to cells incubated with 115 mM NaC1 and 5 mM KC1 lowered cytosolic Ca++ at either low (0.5 mM) extracellular Ca++ (from 194 +/- 14 to 159 +/- 9 nM, p less than .01, N = 6) or high (1.5 mM) extracellular calcium (from 465 +/- 38 to 293 +/- 20 nM, p less than .01, N = 10). This reduction in cytosolic Ca++ was due to high K+ per se and not to changes in tonicity since addition of 55 mM NaC1 was without effect while a similar decrease in cytosolic Ca++ occurred when cells were resuspended in 60 mM NaC1 and 60 mM KC1. PTH release was significantly (p less than .01) greater at 0.5 and 1.5 mM Ca++ in QUIN-2-loaded cells incubated with 60 mM NaC1 and 60 mM KC1 than in those exposed to 115 mM NaC1 and 5 mM KC1. In contrast to most secretory cells, therefore, stimulation of PTH release by high K+ is associated with a decrease rather than an increase in cytosolic Ca++.
mesh_terms: D000634:Aminoquinolines / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D003600:Cytosol / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D010280:Parathyroid Glands / Q000166:cytology* / Q000378:metabolism; D010281:Parathyroid Hormone / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D000634:Aminoquinolines; D005456:Fluorescent Dyes; D010281:Parathyroid Hormone; D012965:Sodium Chloride; C035738:Quin2-acetoxymethyl ester; D011188:Potassium; D002118:Calcium