[Maintenance of a male and a female clone of Schistosoma mansoni by microsurgical transplantation of sporocysts. Reliability of the method].
The long term maintenance (2 years) of a male clone and a female clone of Schistosoma mansoni by microsurgical transplantation of sporocysts allowed us to show that this technique is of great reliability: the postoperative survival of recipient snails at 60 days is greater than 75%; the mean of infection rate of the snails is 75% by the male clone sporocysts and 45% by the female clone sporocysts; the cercarial production of recipient snails is on average 441 cercariae per snail per day for the male clone and 530 cercariae for the female clone; the average worm recovery rate is 24,5%.
D000818:Animals; D002999:Clone Cells; D005260:Female; D008297:Male; D008722:Methods; D051379:Mice; D008974:Mollusca; D012550:Schistosoma mansoni / Q000166:cytology* / Q000235:genetics; D013997:Time Factors
Maintenance d'un clone mâle et d'un clone femelle de Schistosoma mansoni par transplantation microchirurgicale de sporocystes-fils. Fiabilité de la méthode.