Quantitation of phenobarbital and phenobarbital sodium in pharmaceutical dosage forms.
A reverse-phase high-performance liquid chromatographic method for the quantitation of phenobarbital and phenobarbital sodium in pharmaceutical dosage forms (elixir, injection, and tablets) was developed. The method is precise and accurate with percent relative standard deviations of 0.9 (without an internal standard) and 0.7 (with an internal standard) based on six injections. The method is stability indicating and is more sensitive than the revised USP-NF method. The products of decomposition showed two new peaks in the chromatogram.
D002851:Chromatography, High Pressure Liquid; D004304:Dosage Forms; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D010634:Phenobarbital / Q000032:analysis*
D004304:Dosage Forms; D010634:Phenobarbital