title: Inhibitors of lysosomal enzymes: accumulation of lipofuscin-like dense bodies in the brain.
abstract: Injections of leupeptin (a thiol proteinase inhibitor) or chloroquine (a general lysosomal enzyme inhibitor) into the brains of young rats induced the formation of lysosome-associated granular aggregates (dense bodies) which closely resembled the ceroid-lipofuscin that accumulates in certain disease states and during aging. The dense material increased in a dose- and time-dependent fashion and was differentially distributed across brain regions and cell types. These observations provide clues to the origins of ceroid-lipofuscin and suggest means for studying the consequences of its accumulation.
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000648:ultrastructure*; D002738:Chloroquine / Q000494:pharmacology*; D007976:Leupeptins / Q000494:pharmacology*; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009842:Oligopeptides / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007976:Leupeptins; D009842:Oligopeptides; D002738:Chloroquine; C032854:leupeptin