title: Direct spectrophotometric analysis of hemoglobin in isoelectric focusing tube gels.
abstract: Methods are described for the direct spectrophotometric analysis of human oxyhemoglobin, deoxyhemoglobin, and methemoglobin focused in polyacrylamide tube gels. Visible absorption spectra (350-650 nm) of electrofocused hemoglobin bands were recorded using a diode array rapid-scan spectrophotometer. Direct optical sampling of gels allowed the identification of focused hemoglobin valency hybrids which contain two oxidized monomers per tetramer.
mesh_terms: D005782:Gels; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D007525:Isoelectric Focusing; D008706:Methemoglobin / Q000032:analysis; D010108:Oxyhemoglobins / Q000032:analysis; D013053:Spectrophotometry
chemical_list: D005782:Gels; D006454:Hemoglobins; D010108:Oxyhemoglobins; C100003:deoxyhemoglobin; D008706:Methemoglobin