New trends in the rapid serodiagnosis of leptospirosis.
TR/Patoc slide test, ELISA and TIA (Thin-Layer Immunoassay) were evaluated for their use in a rapid serodiagnosis of human leptospirosis. The results obtained indicated that the TR/Patoc slide test is very sensitive and able to detect within a few minutes early antibodies against whichever serovar of leptospires, like the antigens of the Galton test. The value of the ELISA test in detecting antibodies at a low level is confirmed. The TIA assay, employed here for the first time for the diagnosis of leptospirosis, though sensitive seemed too long to perform and consequently it is not a test of choice for a rapid screening of sera.
D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial / Q000592:standards; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007118:Immunoassay; D007919:Leptospira / Q000276:immunology; D007922:Leptospirosis / Q000175:diagnosis* / Q000453:epidemiology; D008403:Mass Screening; D012698:Serologic Tests / Q000639:trends*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial