Autoradiography of ganglioside antigens separated by high-performance thin-layer chromatography with their antibodies.
An improved method is described for the detection of ganglioside antigens with the antibodies on thin-layer chromatograms. The method involves the following steps: Separation of gangliosides on a high-performance thin-layer chromatographic plate, application of antibody solution, and detection of bound antibody with [125I]staphylococcal protein A. Using specific antibodies against gangliosides GM4, GM1, GD3, and asialo GM1 ganglioside, the method allowed positive identification of these antigens on thin-layer plates. It also provides a convenient means of assessing the specificity of an anti-glycolipid antibody.
D000906:Antibodies / Q000302:isolation & purification; D000941:Antigens / Q000302:isolation & purification*; D001345:Autoradiography; D002855:Chromatography, Thin Layer / Q000379:methods; D005677:G(M1) Ganglioside / Q000276:immunology; D005732:Gangliosides / Q000276:immunology* / Q000302:isolation & purification; D006028:Glycosphingolipids / Q000276:immunology; D006801:Humans
D000906:Antibodies; D000941:Antigens; D005732:Gangliosides; D006028:Glycosphingolipids; D005677:G(M1) Ganglioside; C026226:ganglioside, GD3; C028253:ganglioside, GM4; C018835:asialo GM1 ganglioside