title: Ultrastructures of leuco-adsorption on monolayers infected with influenza virus.
abstract: Leuco-adsorption occurring in influenza virus infected-cell cultures was studied morphologically to clarify the mechanisms of adsorption of leukocytes. Among the various types of chicken leukocytes studied, such as lymphocytes, monocytes, granulocytes, and thrombocytes, all were found to adhere to the virus-infected cells. The adsorption seems to occur through at least two processes, one is mediated by microvilli (microvillus-attachment), and the other is direct adherence of both cells (cell-to-cell-attachment). In the former, the leukocytes are bound to the microvilli protruding from the infected MDCK cells and in the latter both cell membranes attach directly. In the cell-to-cell-attachment, there was an electron-lucent gap of about 12 nm in width in the intermembranous space of the junctional regions. This region was similar morphologically to the gap junction. As a result of leucoadsorption no cytolytic effects occurred in the MDCK cells under the experimental conditions.
mesh_terms: D000327:Adsorption; D000818:Animals; D000956:Antigens, Viral; D002448:Cell Adhesion; D002478:Cells, Cultured; D002645:Chickens; D004285:Dogs; D009981:Influenza B virus / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D008854:Microscopy, Electron; D008871:Microvilli / Q000648:ultrastructure; D009976:Orthomyxoviridae Infections / Q000276:immunology*; D011946:Receptors, Antigen
chemical_list: D000956:Antigens, Viral; D011946:Receptors, Antigen