Lipid composition and monooxygenase relationship in liver microsomal membranes from rats fed unbalanced diets.
The cholesterol and phospholipid content of microsomal membrane from rats fed either a high lipid (H1) (30% lard) or a low protein (Lp) diet (6% casein) have been compared with those from rats fed a standard (St) diet (22% casein, 5% lard). For each diet, half of the group was treated with Phenochlor DP6. A significant increase in the phospholipid cholesterol ratio was observed in rats fed a high lipid diet or treated with DP6. These effects tend to increase the microsomal membrane fluidity. The protein deficiency decreased the phospholipid/cholesterol ratio and then the fluidity of the endoplasmic reticulum membrane. The specific activity of cytochrome P-450 to hydroxylate aniline which is independent to the enzymic form of cytochrome P-450 was closely correlated with the viscosity status of the microsomal membrane.
D000815:Aniline Hydroxylase / Q000378:metabolism; D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System; D004041:Dietary Fats / Q000008:administration & dosage*; D050356:Lipid Metabolism; D008297:Male; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism* / Q000648:ultrastructure; D010105:Oxygenases / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D004041:Dietary Fats; D008563:Membrane Lipids; D010743:Phospholipids; D003577:Cytochrome P-450 Enzyme System; D002784:Cholesterol; D010105:Oxygenases; D000815:Aniline Hydroxylase