Measurement of gliclazide in plasma by radial compression reversed-phase liquid chromatography.
We describe a rapid, specific, and precise analysis for gliclazide in plasma by radial compression, reversed-phase, "high-performance" liquid chromatography. Gliclazide and the internal standard, 3-chlorogliclazide, are eluted after 4.4 and 6.8 min, respectively. Only 100 microL of plasma and minimal sample workup are required. The limit of detection for gliclazide in plasma is 0.5 mg/L (1.55 mumol/L) at 229 nm. Precision (CV) of the assay for 10 and 1 mg of gliclazide per litre is 2.1% and 6.4%, respectively.
D000704:Analysis of Variance; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003920:Diabetes Mellitus / Q000097:blood; D005907:Gliclazide / Q000097:blood*; D006801:Humans; D004364:Pharmaceutical Preparations / Q000097:blood; D013453:Sulfonylurea Compounds / Q000097:blood*; D013997:Time Factors
D004364:Pharmaceutical Preparations; D013453:Sulfonylurea Compounds; D005907:Gliclazide