[The use of the annihilated delayed fluorescence phenomenon in the study of the structural organization of the sarcoplasmic reticulum].
Self-interaction of triplet-excited probes and interaction between the triplet-excited probes and a quencher (ferrocene) at 30 degrees C have been studied in Sarcoplasmic reticulum membranes (SR) and in dimiristoyl phosphatidylcholine liposomes by means of annihilated delayed fluorescence (ADF) registration. Perylene, 7,12-dimethyl benzanthracene and 4-(2-anthryl) butanoic acid have been used as triplet probes. An average time decay ADF of perylene and 4-(2-anthryl) butanoic acid in SR is equal to 4,2: 10(-5) s and 2,3 X 10(-5) s, respectively. Triplet states constant quenching of perylene by ferrocene is equal to 2,1 X 10(7) (mM/g of lipids)-1 X s-1 in SR and 4,5 X 10(7) (mM/g of lipids)-1 X s-1 in liposomes. This means that lipids in a liquid crystalline phase form continuous extended regions containing more than 7 X 10(3) lipid molecules and free of significant hindrances in probes diffusion.
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005456:Fluorescent Dyes; D007425:Intracellular Membranes; D007700:Kinetics; D008563:Membrane Lipids; D008565:Membrane Proteins; D008954:Models, Biological; D012519:Sarcoplasmic Reticulum; D014783:Viscosity
D005456:Fluorescent Dyes; D008563:Membrane Lipids; D008565:Membrane Proteins
Ispol'zovanie iavleniia annigiliatsionnoĭ zamedlennoĭ fluorestsentsii dlia issledovaniia strukturnoĭ organizatsii sarkoplazmaticheskogo retikuluma.