Metabolism of methyl n-amyl ketone (2-heptanone) and its binding to DNA of rat liver in vivo and in vitro.
Methyl n-amyl ketone (2-heptanone), a reported metabolite of 2-ethylhexanol which in turn is a primary metabolite of plasticizers such as di-(2-ethylhexyl) phthalate, is metabolized in male Fischer 344 rats to CO2, acetate and a variety of compounds that could be either anabolic or catabolic or a combination of the two. A significant percentage of the radioactivity given orally (gavage) as [2-14C]-2 heptanone, at least 10%, was not excreted from the body in 48 h. Radioactivity was incorporated into liver protein in the form of three unidentified products as well as [14C]arginine, and into DNA both as 14C-labeled normal nucleosides (50-75%) and as presently unidentified hydrophobic materials (25-50%). Urea and cholesterol were significantly labeled, indicative of anabolic reutilization of [2-14C]-2-heptanone breakdown products. The 2-heptanone also bound to DNA spontaneously in vitro, to the extent of 400 pmol/mg DNA.
D000818:Animals; D002250:Carbon Radioisotopes; D002845:Chromatography / Q000379:methods; D002850:Chromatography, Gel; D004247:DNA / Q000378:metabolism*; D003653:Decarboxylation; D004051:Diethylhexyl Phthalate / Q000378:metabolism; D005260:Female; D000441:Hexanols / Q000378:metabolism; D066298:In Vitro Techniques; D007659:Ketones / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D011916:Rats, Inbred F344; D014018:Tissue Distribution
D002250:Carbon Radioisotopes; D000441:Hexanols; D007659:Ketones; C011917:2-heptanone; D004247:DNA; D004051:Diethylhexyl Phthalate; C034017:2-ethylhexanol