Preparation and characterization of radioactive monoiodotyrosine and diiodotyrosine derivatives of parathyroid hormone.
Highly purified native parathyroid hormone was iodinated by the enzymatic method and separated from unlabeled hormone by isocratic HPLC. The separation system used also resolved iodohistidine, monoiodotyrosine, and diiodotyrosine forms of the hormone from one another. A simplified procedure for direct bioassay of the carrier-free, high specific activity, mono- and diiodinated parathyroid hormone (PTH) by the renal membrane adenylyl cyclase method was also developed. Both labeled forms of the hormone are very potent in this assay, but the iodinated forms appeared to give a lower Vmax than the native hormone. The methods for iodination, separation and biological characterization of this PTH tracer are exceptionally facile, inexpensive, and convenient.
D000262:Adenylyl Cyclases / Q000032:analysis; D000818:Animals; D001681:Biological Assay / Q000379:methods; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004105:Diiodotyrosine / Q000032:analysis / Q000138:chemical synthesis; D004591:Electrophoresis, Polyacrylamide Gel; D006639:Histidine / Q000031:analogs & derivatives / Q000032:analysis; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods; D007668:Kidney / Q000201:enzymology; D007470:Monoiodotyrosine / Q000032:analysis / Q000138:chemical synthesis; D010281:Parathyroid Hormone / Q000032:analysis*; D011863:Radioimmunoassay
D007457:Iodine Radioisotopes; D010281:Parathyroid Hormone; C018324:5-iodohistidine; D006639:Histidine; D004105:Diiodotyrosine; D000262:Adenylyl Cyclases; D007470:Monoiodotyrosine