title: Mosquito protein microassay. II. Modification for potential field use.
abstract: A simple microassay is described for determining protein levels in individual mosquitoes; this method should be useful on a routine basis in the field. This method requires only a small portion of a homogenate of a single mosquito. Sugar-fed adult female Anopheles albimanus showed a slow decline in protein level for 14 days post-emergence. Blood volumes and blood-meal protein levels were microassayed, and changes in protein level were monitored throughout the reproductive cycle. Blood-fed Anopheles albimanus protein levels return only slowly to pre-feeding levels following egg deposition.
mesh_terms: D000375:Aging; D000818:Animals; D000852:Anopheles / Q000032:analysis* / Q000254:growth & development; D001769:Blood; D005260:Female; D008297:Male; D008832:Microchemistry; D011506:Proteins / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods
chemical_list: D011506:Proteins