title: Phase-dependent polymerization and depolymerization of actin in Physarum polycephalum nuclei.
abstract: G- and F-actin contents in physarum polycephalum nuclei isolated during the progress of cell cycle were estimated and compared both by the inhibiting activity of deoxyribonuclease I (DNase I) and by the fractionation on DEAE-Toyopearl column chromatography. The inhibition of DNase appeared maximal in late S phase or early G2 phase and decreased to a minimal value in late G2 phase or M phase, while total actin content in a nucleus, which was analyzed by SDS-polyacrylamide gel electrophoresis as well as by DNase inhibition assay in the presence of 0.75 M guanidine-HCl, did not show such phase-dependent dynamics through the cell cycle.
mesh_terms: D000199:Actins / Q000378:metabolism* / Q000494:pharmacology; D002453:Cell Cycle; D002467:Cell Nucleus / Q000378:metabolism; D002850:Chromatography, Gel; D003851:Deoxyribonucleases / Q000037:antagonists & inhibitors; D004591:Electrophoresis, Polyacrylamide Gel; D004983:Ethanolamines; D010804:Physarum / Q000378:metabolism*; D011108:Polymers
chemical_list: D000199:Actins; D004983:Ethanolamines; D011108:Polymers; D003851:Deoxyribonucleases; C007369:2-diethylaminoethanol