title: Accelerated accumulation of aluminum by osteoid matrix in vitamin D deficiency. An animal model of aluminum toxicity.
abstract: To investigate the possibility that aluminum (Al) salts might associate specifically with osteoid matrix in bone, an animal model was developed in which hyperosteoidosis was controlled independently by the vitamin D status. Nonuremic rats were weaned on a vitamin D-free diet (D-) and half were given intraperitoneal AlCl3 (9.25 mg Al over 33 days). At sacrifice, serum calcium was significantly higher in Al-treated D- rats compared to D- controls (9.4 +/- 0.3 vs. 6.9 +/- 0.8 mg/dl, p less than 0.01). Serum and bone Al content were measured by flameless atomic absorption spectroscopy; Al content was 318 +/- 38 micrograms/l in serum, and 248 +/- 11 mg/kg dry bone weight in Al-treated rats, compared to almost undetectable levels in D- controls. Al was identified histochemically in Al-treated rats by its heavy deposition in subepiphyseal trabecular bone; where it occupied almost 50% of the linear trabecular bone surface, usually sandwiched between bone and osteoid seams. Both groups of animals had severe osteomalacia, but there was no evidence that Al induced a more severe lesion in addition to vitamin D deficiency. Parenteral Al was also given to rats fed a similar diet, but supplemented with vitamin D3 (D+) (cumulative dose of Al, 37.25 mg over 70 days). At sacrifice, serum Al was 671 +/- 50 micrograms/l, but bone Al was only 142 +/- 10 mg/kg, significantly less than bone Al in D- Al-treated rats. There was no evidence of osteomalacia in D+ Al-treated rats in which histochemical staining for Al was essentially negative.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000535:Aluminum / Q000097:blood / Q000378:metabolism* / Q000633:toxicity; D000818:Animals; D001835:Body Weight; D001846:Bone Development / Q000187:drug effects; D001857:Bone Matrix / Q000378:metabolism*; D002118:Calcium / Q000097:blood; D004195:Disease Models, Animal; D006651:Histocytochemistry; D008297:Male; D010018:Osteomalacia / Q000378:metabolism; D010710:Phosphates / Q000097:blood; D051381:Rats; D013054:Spectrophotometry, Atomic; D013977:Tibia / Q000187:drug effects / Q000473:pathology; D014808:Vitamin D Deficiency / Q000378:metabolism*
chemical_list: D010710:Phosphates; D000535:Aluminum; D002118:Calcium