Detection of aflatoxin D1 in ammoniated corn by mass spectrometry-mass spectrometry.
Corn cultured with Aspergillus flavus to produce a high level of aflatoxin was ammoniated at 37 degrees C for 21 days. An extract of the ammoniated corn was separated by thin-layer chromatography, followed by reversed-phase high-pressure liquid chromatography. Examination of the fractions by tandem mass spectrometry led to detection of aflatoxin D1 as a product of the corn ammoniation process.
D000348:Aflatoxins / Q000032:analysis*; D000641:Ammonia / Q000494:pharmacology*; D005506:Food Contamination; D013058:Mass Spectrometry; D003313:Zea mays
D000348:Aflatoxins; C029227:aflatoxin D1; D000641:Ammonia