Incorporation of chromaffin granule membranes into large-size vesicles suitable for patch-clamp recording.
Incubation of chromaffin granules with excess liposomes at pH 6.0 resulted in the formation of cell-size structures, which were purified by centrifugation on sucrose gradients. Experiments with fluorescein-labeled granules indicated incorporation of granule membrane to these structures. The preparation contained various vesicular structures with a diameter up to 15 micron. The largest elements were studied by the 'patch-clamp' technique. 'Cell-attached' and 'whole-cell' recordings indicated the presence of currents corresponding to unitary conductances ranging from 100 to 500 pS.
D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002837:Chromaffin Granules / Q000502:physiology / Q000648:ultrastructure*; D002838:Chromaffin System / Q000648:ultrastructure*; D004594:Electrophysiology; D008081:Liposomes; D008854:Microscopy, Electron
D008081:Liposomes