[Effect of gamma irradiation on immobilized trypsin].
The effect of ionizing radiation of 0.05-10 Mrad on trypsin immobilized on dialdehyde cellulose was being studied. After irradiation the activity of native trypsin decreases by 25%, as compared with the initial, while the activity of immobilized trypsin remains constant. Before immobilization cellulose undergoes special pretreatment that leads to a decrease in the initial contamination. Some samples of modified cellulose were contaminated by staphylococcus culture (200,000 microbes per 0.2 g) and then exposed to irradiation of 0.05-0.4 Mrad. A distinct correlation between the irradiation dose (0.05-0.4 Mrad) and contamination of the object was registered.
D001458:Bandages; D002364:Caseins / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D004800:Enzymes, Immobilized / Q000378:metabolism / Q000528:radiation effects*; D005720:Gamma Rays; D066298:In Vitro Techniques; D013210:Staphylococcus / Q000187:drug effects / Q000528:radiation effects; D013242:Sterilization; D014357:Trypsin / Q000378:metabolism / Q000528:radiation effects*
D002364:Caseins; D004800:Enzymes, Immobilized; D014357:Trypsin
Vliianie gamma-oblucheniia na immobilizovannyĭ tripsin.