title: Fibronectin synthesis during oogenesis and early development of the amphibian Pleurodeles waltlii.
abstract: Fibronectin (FN) synthesis during oogenesis and early embryogenesis of the amphibian Pleurodeles waltlii was investigated. The isotopically labelled amino acids [3H]leucine and [35S]methionine were incubated with oocytes or microinjected into embryos. Newly synthesized FN was analysed by polyacrylamide gel electrophoresis, using the high resolution two-dimensional gel system described by O'Farrell. With this method and fluorography we demonstrate that FN synthesis begins during oogenesis. De novo synthesized FN appears during cleavage and gastrulation. Using actinomycin D we show the presence of maternal messenger RNA coding for FN. It is translated during the cleavage and gastrulation stages.
mesh_terms: D000818:Animals; D003609:Dactinomycin / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000096:biosynthesis*; D007526:Isoelectric Point; D008970:Molecular Weight; D009866:Oogenesis; D010999:Pleurodeles / Q000196:embryology* / Q000378:metabolism; D012455:Salamandridae / Q000196:embryology*
chemical_list: D005353:Fibronectins; D003609:Dactinomycin