title: A cold stage for the Philips EM300 electron microscope.
abstract: A cold stage has been constructed for the Philips EM300 (and EM301) electron microscopes for investigating the structure of frozen-hydrated biological specimens. The stage entails minimal alterations to the instrument and is capable of a resolution better than 10 A at the normal operating temperature of -120 degrees C. Frozen specimens can be readily exchanged without condensation or warming up, and maintained in the stage over periods of several hours without detectable deterioration.
mesh_terms: D005615:Freezing; D008854:Microscopy, Electron / Q000379:methods*