Use of bone marrow fibroblasts to prepare targets for an HLA restricted-cytotoxicity assay system.
Methods are described for obtaining and growing fibroblasts from bone marrow for use as virus-infected targets. Fibroblasts obtained at the time of routine marrow examination were maintained through 12 to 18 passages as confluent monolayers. Bone marrow fibroblasts could be infected with varicella-zoster virus, and these infected cells were suitable targets for a 51Cr-release cytotoxicity assay. Since these virus-infected cells retain their HLA-A and -B antigens, they are readily available to study the immune cells which mediate virus-specific cytotoxicity.
D000293:Adolescent; D000328:Adult; D001853:Bone Marrow / Q000382:microbiology* / Q000648:ultrastructure; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D002877:Chromosomes, Human; D003470:Culture Media; D003601:Cytotoxicity Tests, Immunologic; D005347:Fibroblasts / Q000382:microbiology; D006680:HLA Antigens / Q000276:immunology*; D006562:Herpes Zoster / Q000276:immunology; D006801:Humans
D003470:Culture Media; D006680:HLA Antigens