Ultraviolet light and mitomycin C induced sister-chromatid exchanges in fibroblasts from patients with retinoblastoma.
Ultraviolet light and mitomycin C (MMC) induced sister-chromatid exchanges (SCEs) were investigated in 6 diploid fibroblast strains derived from 3 patients with deletion 13 and retinoblastoma, one patient with a hereditary form of retinoblastoma, one patient with trisomy 13, and one normal control. Two fibroblast strains with del(13)(q14q22) showed a significant increase in SCEs compared to the control after UV and MMC treatments. In contrast, cell strains with del(13)(q12q14) and trisomy 13 did not show increased SCEs. The frequency of SCEs in fibroblasts from a patient with autosomal dominant retinoblastomas (no deletions) was significantly increased by UV, but not by MMC. The results suggest that cell strains with different deletions of chromosome 13 have different SCE responses to UV and MMC inductions. The cells with del(13)(q14q22) may have a DNA-repair defect.
D002872:Chromosome Deletion; D002901:Chromosomes, Human, 13-15; D004260:DNA Repair; D006801:Humans; D008937:Mitomycins / Q000494:pharmacology*; D012175:Retinoblastoma / Q000235:genetics*; D012854:Sister Chromatid Exchange / Q000187:drug effects / Q000528:radiation effects*; D014314:Trisomy; D014466:Ultraviolet Rays
D008937:Mitomycins