title: Functional evidence for distinct interaction of hydrophobic arylisothiocyanates with the erythrocyte anion transport protein.
abstract: Human erythrocytes were treated with various hydrophobic arylisothiocyanates under conditions which favor modification of distinct proteinaceous nucleophiles. The morphological appearance of phenylisothiocyanate-treated cells was discoid and membrane-bound hydrolases (human acetylcholinesterase, sheep phospholipase A2) were fully active following membrane modification. Noncharged hydrophobic arylisothiocyanates, including phenylisothiocyanate, beta-naphthylisothiocyanate and heterobifunctional azidoarylisothiocyanates inhibited [35S]-sulfate efflux irreversibly. Protection against modification-induced inhibition of sulfate transport was attained by the simultaneous presence of the specific reversible anion transport inhibitor 4,4'-dinitrostilbene-2,2'-disulfonate. Selective protection of a functionally relevant domain of band 3 is concluded to occur based on the above-derived information.
mesh_terms: D015058:1-Naphthylisothiocyanate / Q000494:pharmacology; D000110:Acetylcholinesterase / Q000097:blood; D001457:Anion Exchange Protein 1, Erythrocyte / Q000097:blood; D027321:Anion Transport Proteins; D002352:Carrier Proteins / Q000097:blood*; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D006801:Humans; D017879:Isothiocyanates; D010741:Phospholipases A / Q000097:blood; D054467:Phospholipases A2; D013267:Stilbenes / Q000494:pharmacology; D013431:Sulfates / Q000097:blood; D013861:Thiocyanates / Q000494:pharmacology*
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D027321:Anion Transport Proteins; D002352:Carrier Proteins; D017879:Isothiocyanates; D013267:Stilbenes; D013431:Sulfates; D013861:Thiocyanates; C005441:phenylisothiocyanate; D015058:1-Naphthylisothiocyanate; C022306:4,4'-dinitro-2,2'-stilbenedisulfonic acid; D010741:Phospholipases A; D054467:Phospholipases A2; D000110:Acetylcholinesterase; C031287:2-naphthylisothiocyanate