Elemental profiles in cryosections and frozen-dried bulk specimens of the normal lens.
Energy-dispersive X-ray analysis was used to obtain distribution profiles of Na, K, Cl, P and S in rapidly frozen-dried bulk specimens, and in cryosections of the normal rat lens. Na and Cl are highly concentrated in the capsule, and K and P, in the epithelial cells. Profiles of the entire radius of a lens show relatively high concentrations of K and P in the outermost cells of the lens, with decreasing concentrations through the cortex, and a minimum throughout the nuclear region. S, on the other hand, shows increasing concentrations at deeper locations, with maximum values throughout the nuclear region.
D000818:Animals; D003452:Cryosurgery; D004577:Electron Probe Microanalysis; D004602:Elements / Q000032:analysis*; D004848:Epithelium / Q000032:analysis; D005612:Freeze Drying; D007477:Ions / Q000032:analysis; D007903:Lens Capsule, Crystalline / Q000032:analysis; D007904:Lens Cortex, Crystalline / Q000032:analysis; D007908:Lens, Crystalline / Q000032:analysis*; D051381:Rats; D013057:Spectrum Analysis
D004602:Elements; D007477:Ions