The molecular characteristics of a human pancreatic acidic phosphoprotein that inhibits calcium carbonate crystal growth.
A CaCO3-crystal-growth inhibitor was isolated from human pancreatic stones by using EDTA demineralization, followed by DEAE-Trisacryl chromatography. The isolated inhibitor was found to be a phosphoglycoprotein with Mr 14017 and having an unusual chemical composition. It is characterized by a high (42%) acidic amino acid content, but lacks methionine and gamma-carboxyglutamic acid. The protein contains 2.65 mol of P/mol of protein, as phosphoserine (2 mol) and phosphothreonine (0.5 mol). Isoelectric focusing of the protein yields one major band corresponding to an isoelectric point of 4.2. Immunochemical quantification of the crystal-growth inhibitor in pure pancreatic juice reveals that it constitutes 14% of the normal exocrine secretion. Our findings demonstrate that this is a novel secretory protein, which has no enzymic activity and which maintains pancreatic juice in a supersaturated state with respect to CaCO3.
D000596:Amino Acids / Q000032:analysis; D002119:Calcium Carbonate; D002135:Calcium-Binding Proteins; D002137:Calculi / Q000378:metabolism*; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003460:Crystallization; D006801:Humans; D007525:Isoelectric Focusing; D051847:Lithostathine; D009419:Nerve Tissue Proteins; D010182:Pancreatic Diseases / Q000378:metabolism*; D010189:Pancreatic Juice / Q000032:analysis; D010710:Phosphates / Q000032:analysis
D000596:Amino Acids; D002135:Calcium-Binding Proteins; D002241:Carbohydrates; D051847:Lithostathine; D009419:Nerve Tissue Proteins; D010710:Phosphates; C497617:REG1A protein, human; D002119:Calcium Carbonate