Evidence that the cytosolic activity of 3-hydroxybutyrate dehydrogenase in chicken liver is L-3-hydroxyacid dehydrogenase.
Classical fractionation studies showed that chicken liver contains two enzymes which can oxidize DL-3-hydroxybutyrate. The cytosolic enzyme is specific for the L-(+) isomer and accounts for 60% of the total activity. The mitochondrial activity is specific for the D-(-) isomer and accounts for 40% of the total activity. Kinetic studies showed that L-gulonic acid is a competitive inhibitor of the enzyme. We conclude that the cytosolic enzyme is the previously described L-3-hydroxyacid dehydrogenase.
D020155:3-Hydroxybutyric Acid; D000818:Animals; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D002645:Chickens; D003600:Cytosol / Q000201:enzymology; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism; D007536:Isomerism; D008099:Liver / Q000201:enzymology*; D008297:Male; D013379:Substrate Specificity; D014018:Tissue Distribution
D006885:Hydroxybutyrates; D002237:Carbohydrate Dehydrogenases; D006884:Hydroxybutyrate Dehydrogenase; C020843:gulonate dehydrogenase; D020155:3-Hydroxybutyric Acid