Effect of indomethacin in vivo on prostaglandin content of several rabbit tissues.
The prostaglandin (PG) content of several tissues and fluids from 6 day pregnant rabbits was evaluated following treatment with indomethacin or vehicle in vivo. PGE and PGF were measured by radioimmunoassay. More complete depletion of PGE and PGF was accomplished by 3 injections of indomethacin (s.c.) given during the 18 h before sacrifice at a dose of 10 mg indomethacin per kg body weight than was accomplished by 1 injection of the same amount of indomethacin (i.v.) 1.5 h before sacrifice. Levels of PGF were more easily depressed by indomethacin than were those of PGE. PG levels in the kidney and blastocysts were depressed to a greater extent by indomethacin than were those in the uterus, uterine fluid or peritoneal fluid. Evaluation of the effect of indomethacin on a particular physiological function should be interpreted with caution unless the extent of PG depletion in that tissue is also measured.
D000818:Animals; D001755:Blastocyst / Q000032:analysis; D005260:Female; D007213:Indomethacin / Q000494:pharmacology*; D007668:Kidney / Q000032:analysis; D010529:Peritoneal Cavity / Q000032:analysis; D011247:Pregnancy; D011458:Prostaglandins E / Q000032:analysis*; D011460:Prostaglandins F / Q000032:analysis*; D011817:Rabbits; D013997:Time Factors; D014018:Tissue Distribution; D014599:Uterus / Q000032:analysis
D011458:Prostaglandins E; D011460:Prostaglandins F; D007213:Indomethacin