A common 43 K protein induced by glucocorticoids in a variety of cells and tissues.
We have investigated the domain of adrenal steroid action in a variety of mammalian cells and tissue by high resolution two-dimensional gel electrophoresis and autoradiography. Following in vivo or in vitro treatment with steroids, minced tissue or cells were pulsed with [35S]methionine, and they newly synthesized polypeptides compared with controls by visual inspection of partial protein maps. We have found a similar protein (Mr approximately 41-44K, pI approximately 6.3-6.5) to be consistently increased by dexamethasone in 3 rat tissues and 9 human, bovine and rat cell lines. The protein was constitutively synthesized in all targets; quantitative measurements on the magnitude of response show a 2-fold induction by dexamethasone in all systems, in vivo and in vitro. Glucocorticoid-specific hormones but not sex steroids increase the rate of synthesis; deoxycorticosterone has agonist or antagonist effects on 43K synthesis in different systems studied. Co-electrophoresis experiments indicated that 43K proteins co-migrate in at least 3 rat cell lines from tissues with diverse functions. The functional significance of this common glucocorticoid-induced protein is unknown; its ubiquity and electrophoretic properties suggest a highly conserved, common indicator of glucocorticoid action in diverse target cells and tissues, rather than a tissue-specific response.
D000732:Androstanols / Q000494:pharmacology; D000818:Animals; D017302:Annexins; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002135:Calcium-Binding Proteins; D002417:Cattle; D003900:Desoxycorticosterone / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology*; D006023:Glycoproteins; D012739:Gonadal Steroid Hormones / Q000494:pharmacology; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D014176:Protein Biosynthesis; D011506:Proteins / Q000494:pharmacology; D051381:Rats
D000732:Androstanols; D017302:Annexins; D000893:Anti-Inflammatory Agents; D002135:Calcium-Binding Proteins; D005938:Glucocorticoids; D006023:Glycoproteins; D012739:Gonadal Steroid Hormones; D011506:Proteins; C030958:lipomodulin; D003900:Desoxycorticosterone; C038341:RU 26988; D003907:Dexamethasone