Strand separation of DNA fragments and their isolation from nondenaturing polyacrylamide gels.
A technique for rapidly and quantitatively denaturing double-stranded DNA employing urea and moderate heat is described. The single DNA strands are resolved on high-percentage nondenaturing polyacrylamide gels from which they can be recovered for Maxam-Gilbert sequence analysis.
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D009691:Nucleic Acid Denaturation; D013552:Swine; D014508:Urea
D004277:DNA, Single-Stranded; D014508:Urea