Biochemical study of cholesteatoma and cholesterol granuloma--occurrence of delta 7-cholestenol in the tissues of cholesteatoma.
Qualitative and quantitative analyses of sterol derivatives in the tissue of cholesteatoma and cholesterol granuloma were performed by gas-liquid chromatography, mass spectrometry and mass fragmentography after the extraction of total lipids with organic solvents. Cholesterol was the major component in both tissues. The molar ratios of cholesterol to lipid-bound phosphorus in cholesterol granulomas were higher than those in cholesteatomas. In addition, delta 7-cholestenol (lathosterol) was contained in all tissues of cholesteatoma examined. However, cholesterol granuloma tested did not contain delta 7-cholestenol, indicating that the metabolism of cholesterol differed from that in cholesterol granuloma.
D055598:Chemical Phenomena; D002621:Chemistry; D002781:Cholesteatoma / Q000378:metabolism*; D002784:Cholesterol / Q000032:analysis*; D004427:Ear Diseases / Q000378:metabolism*; D004432:Ear, Middle / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006099:Granuloma / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry
C001521:lathosterol; D002784:Cholesterol