Molecular sizing of individual polysaccharides in polyvalent pneumococcal and meningococcal vaccines by column chromatography and immunoelectrophoresis.
We determined the molecular size of meningococcal and pneumococcal polysaccharides in final vaccines, using a combination of column chromatography and simple rocket immunoelectrophoresis. We found a good correlation between the distribution coefficients obtained with UV monitoring or ELISA and immunoelectrophoresis.
D001428:Bacterial Vaccines; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006358:Hot Temperature; D007122:Immunoelectrophoresis; D022401:Meningococcal Vaccines; D008970:Molecular Weight; D022242:Pneumococcal Vaccines; D011135:Polysaccharides, Bacterial
D001428:Bacterial Vaccines; D022401:Meningococcal Vaccines; D022242:Pneumococcal Vaccines; D011135:Polysaccharides, Bacterial