title: Measurements of glutathione and other thiols in cells and tissues: a simplified procedure based on the HPLC separation of monobromobimane derivatives of thiols.
abstract: Although there is intense interest in the role of thiols in controlling the efficiency of radiosensitizers, and in developing thiols (or pro-drugs liberating thiols) as radioprotectors, there is little information regarding the concentration of specific thiols in cells, tumors and normal tissues. Details are presented of a modified procedure using the thiol binding agent monobromobimane with separation using paired-ion reverse phase high performance liquid chromatography (HPLC). This method has been extended to include measurements of the radiosensitizer misonidazole and its desmethylated metabolite Ro 05-9963 in tissues.
mesh_terms: D000818:Animals; D001643:Bridged Bicyclo Compounds; D001952:Bridged-Ring Compounds; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D005978:Glutathione / Q000032:analysis*; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D008624:Mercaptoethylamines / Q000032:analysis; D051379:Mice; D008920:Misonidazole / Q000031:analogs & derivatives / Q000032:analysis; D009132:Muscles / Q000032:analysis; D009374:Neoplasms, Experimental / Q000032:analysis; D011838:Radiation-Sensitizing Agents / Q000032:analysis; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D001643:Bridged Bicyclo Compounds; D001952:Bridged-Ring Compounds; D008624:Mercaptoethylamines; D011838:Radiation-Sensitizing Agents; D013438:Sulfhydryl Compounds; C020174:N-(2-mercaptoethyl)-1,3-diaminopropane; D008920:Misonidazole; D005978:Glutathione; C009997:desmethylmisonidazole; C028381:monobromobimane