[Calculation of Steel's cell loss factor and of the parameters of cellular kinetics for a population with an exponential growth of the cell number and cell death at G0 phase with a probability equal to 1].
The method of calculation of three cell kinetics parameters (the Steel's cell loss factor phi, the proliferative pool Pc, and the mean number m of the proliferating cells after mitotic division of one cell) was shown to be the same for the exponential growth state of cell number with cell death at the G0-phase, and for the exponential growth state with cell death occurring immediately after mitosis. The value of the mean number delta of non-proliferating cells that appeared after mitotic division of one cell is different for these two models of the exponential growth state with the equal values of the other three parameters (phi, Pc, and m). A method is proposed for calculating the parameter delta on the data of the percentage of labeled cells obtained in the experiments with continuous cultivation of cells in the nutrient medium containing 3H-thymidine. The kinetics of cell line HL-60 (the experimental data of Foa et al., 1982) can be described at the first approximation, by a model of the exponential growth state with the cell death at the G0-phase, with Pc = 0.80, phi = 0.24, m = 1.61, delta = 0.39, and the life time of the non-proliferating cells tQ = 24 hours.
D002452:Cell Count; D002460:Cell Line; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D002478:Cells, Cultured; D006801:Humans; D007399:Interphase; D007700:Kinetics; D007938:Leukemia / Q000473:pathology; D008433:Mathematics; D008954:Models, Biological; D011336:Probability
Raschet factora kletochnykh poter' Stila i nekotorykh parametrov kletochnoĭ kinetiki dlia populiatsii s éksponentsial'nym rostom chisla kletok i gibel'iu kletok v faze G0 s veroiatnost'iu, ravnoĭ 1.