Subcellular glutathione contents in isolated hepatocytes treated with L-buthionine sulfoximine.
The glutathione contents of the mitochondrial and cytosolic fractions and extracellular space of isolated hepatocytes decrease when glutathione synthesis is inhibited with L-buthionine sulfoximine. Mitochondrial glutathione is depleted to 50% of its initial value whereas the cytosolic pool is completely emptied after 2 h incubation in the presence of inhibitor. The mitochondrial glutathione content was only fully depleted when L-buthionine sulfoximine was added together with phorone (2,6-dimethyl-2,5-heptadiene-4-one), a substrate of the glutathione S-transferases (E.C. 2.5.1.18).
D000818:Animals; D019328:Buthionine Sulfoximine; D003600:Cytosol / Q000378:metabolism; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism*; D005980:Glutathione Reductase / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; D004228:Dithionitrobenzoic Acid; D005980:Glutathione Reductase; D005978:Glutathione