Non-specific binding of heat-aggregated IgG to histone detected by ELISA.
Anti-histone antibodies are currently detected by micro ELISA in systemic lupus erythematosus sera from humans, mice and dogs. Here we show that the control-heated sera may bind non-specifically to the whole histones and histone fractions. The heated immunoglobulins binding to histones are mainly IgG and to a lesser extent IgA, but never IgM. These false positive ELISA reactions occurred only with aggregated IgG which binds to histones via Fc; IgM rheumatoid factor prevented their fixation. Immune complexes do not seem to interfere significantly in the detection of anti-histone antibodies with the ELISA test.
D000906:Antibodies / Q000032:analysis*; D000936:Antigen-Antibody Complex / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006657:Histones / Q000032:analysis* / Q000276:immunology; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D011485:Protein Binding; D013745:Tetanus Toxoid / Q000276:immunology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D006657:Histones; D007074:Immunoglobulin G; D013745:Tetanus Toxoid