Use of bovine parathyroid hormone, labelled specifically in the N-terminal region by [3H]methyl exchange, to compare hepatic and renal metabolism of this hormone in the rat.
[3H]bPTH, prepared by [3H]methyl exchange, was administered intravenously to rats. Analysis of kidney and liver extracts on Sephadex G-50 showed some low mol.wt (peak IV) and some bound (peak Ia) material. A peak of intermediate mol.wt (peak II) was present in kidney but absent from liver. Kidneys that had been perfused with [3H]bPTH contained similar metabolites to those described, indicating that they are generated de novo. Analysis of peak II by reverse-phase h.p.l.c. showed a number of metabolites. The significance of these findings is discussed. This study demonstrates the value of [3H]methyl exchange labelling in investigating peptide metabolism.
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D005260:Female; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D009928:Organ Specificity; D010281:Parathyroid Hormone / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D010281:Parathyroid Hormone; D014316:Tritium