Hydrogenation of triton X-100 eliminates its fluorescence and ultraviolet light absorption while preserving its detergent properties.
The ultraviolet-light absorption and fluorescence of Triton X-100 were virtually eliminated by hydrogenation to its reduced cyclohexyl analog, RTX-100. The critical micelle concentration of RTX-100 was 12% higher than that of Triton X-100. RTX-100 and Triton X-100 were quite similar in their abilities to extract proteins from human erythrocyte membranes.
D002855:Chromatography, Thin Layer; D003902:Detergents; D004910:Erythrocyte Membrane / Q000032:analysis; D006801:Humans; D008565:Membrane Proteins / Q000302:isolation & purification; D008823:Micelles; D017830:Octoxynol; D011092:Polyethylene Glycols; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents
D003902:Detergents; D008565:Membrane Proteins; D008823:Micelles; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D017830:Octoxynol; C043504:RTX 100