Quantitative and qualitative effects of N10-methylfolate on high-affinity folate binding in human leukocytes.
N10-methylfolate acted as a potent competitive inhibitor of high-affinity [3H] folate binding in human leukocytes, while methotrexate had no effect. Furthermore, folate binding changed into a non-cooperative type in the presence of N10-methylfolate. Hence, in qualitative and quantitative respects, the substrate specificity characteristics of leukocyte folate binding resemble those of other high-affinity folate binding systems.
D001667:Binding, Competitive; D005492:Folic Acid / Q000097:blood*; D006801:Humans; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism*; D008727:Methotrexate / Q000031:analogs & derivatives* / Q000494:pharmacology
C028162:N(10)-methylfolate; D005492:Folic Acid; D008727:Methotrexate