Glutathione conjugation of arylnitroso compound: detection and monitoring labile intermediates in situ inside a fast atom bombardment mass spectrometer.
The glutathione (GSH) conjugation reaction of the active metabolite of a potent protein-pyrolysate carcinogen, 2-nitroso-6-methyldipyrido[1,2-a:3',2'-d]imidazole (NO-Glu-P-1), occurred in glycerol matrix inside a fast atom bombardment (FAB) mass spectrometer. The short lived GSH-conjugates were detected by in situ FAB analysis. The precursor-product relationship between the conjugates was indicated by following the reaction by measuring [M + H]+ ions of the conjugates.
D001711:Biotransformation; D002273:Carcinogens; D055598:Chemical Phenomena; D002621:Chemistry; D005978:Glutathione / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D009603:Nitroso Compounds / Q000378:metabolism
D002273:Carcinogens; D009603:Nitroso Compounds; C042871:2-nitroso-6-methyldipyrido(1,2-a-3',2'-d)imidazole; D005978:Glutathione