title: The sugar spectrum of human cholesterol gallstones, mixed and pigment gallstones: combined quantitative analysis of neutral sugars, N-acetylhexosamines, hexuronic and N-acetylneuraminic acids by capillary gas-liquid chromatography.
abstract: Glycoproteins were investigated in different types of biliary calculi by a methanolysis procedure of the carbohydrate moiety, the formation of 2,6-dinitro-4-trifluoromethylbenzenesulphonate derivatives of N-acetylhexosamines and N-acetylneuraminic acid, followed by separation, identification and quantification of the liberated monosaccharides using capillary gas-liquid chromatography. This indirect method avoids several analytical problems caused by the presence of bile pigment derivatives; unless these are carefully removed they interfere strongly in the measurement of protein. The carbohydrate contents were much higher in pigment stones than in cholesterol and mixed stones. Rhamnose, a non-mammalian monosaccharide, was identified in most samples, but was significant increased in pigment stones, indicating a bacterial history of this stone type.
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D002769:Cholelithiasis / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism*; D002849:Chromatography, Gas / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006595:Hexosamines / Q000032:analysis; D006603:Hexuronic Acids / Q000032:analysis; D006801:Humans; D006868:Hydrolysis; D008697:Methane; D010860:Pigments, Biological / Q000032:analysis; D012794:Sialic Acids / Q000032:analysis; D013213:Starch / Q000032:analysis
chemical_list: D002241:Carbohydrates; D006595:Hexosamines; D006603:Hexuronic Acids; D010860:Pigments, Biological; D012794:Sialic Acids; D013213:Starch; D002784:Cholesterol; D008697:Methane