Isolation of patient-related antigens from allergen extracts by means of protein A-sepharose-patient IgG1,2,4 bio-affinity chromatography.
A method is described for bio-affinity purification of patient-specific IgG reactive antigens by using protein A-sepharose allergic patient serum and the corresponding antigen/allergen extract. The optimal conditions for each step have been determined and it is shown that the non-specific binding of both serum proteins and antigens can be kept at very low levels by a high ionic strength. The specificity of the reaction has been shown by biospecific elution of 125I-labelled antigens by non-labelled antigens and also by comparison of the amount of antigen bound by sera from hyposensitized patients with that from non-atopic individuals. It is suggested that the isolated antigen-IgG-immune complexes could be used for a specific and active immunotherapy.
D000485:Allergens / Q000302:isolation & purification*; D000936:Antigen-Antibody Complex / Q000302:isolation & purification; D000941:Antigens / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*
D000485:Allergens; D000936:Antigen-Antibody Complex; D000941:Antigens; D007074:Immunoglobulin G