[Serum assay of anti-wasp venom IgG by radioimmunoprecipitation. Application to patients during desensitization].
Rapid, specific and reproducible radioimmunoprecipitation assay (RIP) for measuring IgG antibodies against Yellow Jacket Venom (YJV) has been developed. The YJV-I125 is added to serum sample and the immune complexes are precipitated by a second antibody with polyethylene glycol. IgG antibodies were measured by RIP in 24 patients receiving Yellow Jacket Venom immunotherapy. RIP assays were compared for 159 sera to a solid phase procedure (RAST IgG).
D000936:Antigen-Antibody Complex / Q000276:immunology; D001514:Bee Venoms / Q000276:immunology*; D011232:Chemical Precipitation / Q000379:methods; D003888:Desensitization, Immunologic; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology* / Q000628:therapy; D007120:Immunochemistry; D007074:Immunoglobulin G / Q000032:analysis*; D007457:Iodine Radioisotopes; D014862:Wasp Venoms / Q000276:immunology*
D000936:Antigen-Antibody Complex; D001514:Bee Venoms; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D014862:Wasp Venoms
Dosage sérique des IgG anti-venin de guêpe par radioimmunoprecipitation (RIP-IgG). Application à des sujets en cours de désensibilisation.