title: Inhibition of human polymorphonuclear leukocyte cell responses by ibuprofen.
abstract: The stimulation of cell swelling, cell aggregation, polymorphonuclear leukocyte locomotion, and lysosomal enzyme release in response to chemoattractant were all inhibited by ibuprofen, a nonsteroidal anti-inflammatory agent. The dosages needed to induce 50% inhibition (ID50) were 5.9, 7.6, 60, and 95 micrograms/mL, respectively. Aside from the differences in ID50, there was also a difference in the degree of maximum inhibition (Imax) of the complement C5a-stimulated responses observed, so that at achievable serum drug concentrations of 20-50 micrograms/mL, inhibition of 67-78% for cell swelling, 69-82% for cell aggregation, 20-35% for migration response, and 17-38% for lysosomal enzyme release were demonstrated. Also observed were a minor stimulatory effect on nitroblue tetrazolium reduction and an inhibitory effect on the ability to kill Staphylococcus aureus, but only at very high concentrations (approximately 2 mg/mL).
mesh_terms: D001770:Blood Bactericidal Activity; D002449:Cell Aggregation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002630:Chemotactic Factors / Q000494:pharmacology; D003182:Complement C5 / Q000494:pharmacology; D015936:Complement C5a; D005260:Female; D006801:Humans; D007052:Ibuprofen / Q000494:pharmacology*; D066298:In Vitro Techniques; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects*; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D013211:Staphylococcus aureus / Q000502:physiology
chemical_list: D002630:Chemotactic Factors; D003182:Complement C5; D009580:Nitroblue Tetrazolium; D015936:Complement C5a; D007052:Ibuprofen