Neurophysin heterogeneity. Difference between newly formed and aged neurosecretory granules.
Neurosecretory granules from bovine neurohypophyses were isolated on iso-osmotic gradients. The content of the granules was analyzed by analytical and two-dimensional gel electrophoreses. The distributions in the gels of vasopressin precursor and neurophysins were detected by radioimmunoassays. Analytical gel electrophoresis of the content of a crude granule preparation showed the presence of different populations of neurophysin molecules. Further analysis demonstrated that vasopressin-neurophysin and oxytocin-neurophysin can be subdivided into molecules with different pI values. Whereas newly formed granules showed two main spots of neurophysin with pI of 5.0 and 5.6, aged granules contain in addition to these different populations of neurophysin-like material, some of which had a basic pI. Vasopressin precursor activity was detected in spots containing proteins with acidic pI and Mr approximately 18,000 and also in proteins of Mr = 8,000-10,000 migrating in the basic region of the gel. The results suggest that in the neural lobe there is an aging process which gives rise to several subpopulations of neurophysins. The different forms of vasopressin-associated bovine neurophysin and oxytocin-associated bovine neurophysin are only found in the granules which are not required for release.
D000818:Animals; D002417:Cattle; D002458:Cell Fractionation; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009481:Neurophysins / Q000096:biosynthesis / Q000302:isolation & purification*; D010904:Pituitary Gland, Posterior / Q000648:ultrastructure*; D011498:Protein Precursors / Q000302:isolation & purification; D014667:Vasopressins / Q000096:biosynthesis
D009481:Neurophysins; D011498:Protein Precursors; D014667:Vasopressins