title: Sequence specificity of cytosine methylation in the DNA of the Chinese hamster ovary (CHO-K1) cell line.
abstract: We have determined the DNA renaturation kinetics for those DNA sequences of the Chinese hamster ovary (CHO-K1) cells in which enzymatic cytosine methylation occurred immediately after strand synthesis and for those in which methylation was delayed after strand synthesis. DNA sequences showing immediate or delayed methylation were found to be distributed throughout all repetition classes of the DNA of these cells, with a slight concentration of immediate methylation in moderately repetitive sequences and with delayed methylation being slightly over-represented in the highly repetitive fraction. However, DNA sequences showing both classes of methylation were represented equally in unique DNA sequences. We interpret these data to mean that the methylase acting near the replication forks (the 'immediate' methylase) is a relatively inefficient enzyme, missing some 20% of hemimethylated sites produced by DNA replication in these cells. We suggest that the methylase performing maintenance methylation at sites remote from the replication forks (the 'delayed' methylase) is simply a back-up enzyme for the first and that it has no true sequence specificity. The implications of this for the function(s) of DNA methylation in mammalian cells are discussed.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002250:Carbon Radioisotopes; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D003596:Cytosine / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D008745:Methylation; D009695:Nucleic Acid Renaturation; D010053:Ovary; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D014316:Tritium; D003596:Cytosine; D004247:DNA; D008715:Methionine; D013936:Thymidine