Fluorometric determination of malonic acid with dicyclohexylcarbodiimide.
By using dicyclohexylcarbodiimide (DCC) as a reagent, a fluorometric method for the determination of malonic acid has been developed. The reagent combined with the acid to afford N,N-dicyclohexylbarbituric acid which reacted with glutaconic aldehyde, generated from the reaction of DCC with pyridine, to form a fluorophore. The fluorophore was then determined at lambda ex = 469 nm and lambda em = 562 nm. The calibration curve was linear from 2.5 to 25 nmol. The relative standard deviation obtained with 10 nmol of malonic acid was 5.77% (n = 10). The specificity is also discussed.
D002234:Carbodiimides; D002384:Catalysis; D004024:Dicyclohexylcarbodiimide; D008314:Malonates / Q000032:analysis*; D012997:Solvents; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013997:Time Factors
D002234:Carbodiimides; D008314:Malonates; D012997:Solvents; D004024:Dicyclohexylcarbodiimide; C030290:malonic acid