[Characteristics of the proliferative activity of the aggregates forming during recombination of the epithelium and mesenchyma of embryonic lungs from intact and carcinogen-treated mice].
Methods of separation of the mouse embryonic lung mesenchyme (M) and epithelium (E) and obtaining homolinear and heterolinear organotypic aggregates of E and M from the intact and urethane-treated (transplacentally) A and C57BL mouse embryos. In the aggregates formed by E and M from the experimental A embryos, the index of labelled nuclei (3H-thymidine incorporation) in both the tissue components was several times that in the aggregates of E and M from the intact embryos. M from the experimental embryos aggregated with E from the intact embryos increased the proliferative activity of the latter 4 to 11 times, whereas M of the intact embryos aggregated with E of the experimental embryos decreased its proliferative activity to the normal level. A suggestion is put forward that the embryonic lung mesenchyme plays an important role in the realization of transplacental carcinogenic influences and in the development of lung epithelial tumours.
D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects / Q000196:embryology; D008168:Lung / Q000187:drug effects / Q000196:embryology*; D008175:Lung Neoplasms / Q000139:chemically induced / Q000196:embryology; D008648:Mesoderm / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D014520:Urethane / Q000494:pharmacology*
D014520:Urethane
Osobennosti proliferativnoĭ aktivnosti agregatov, obrazuiushchikhsia pri rekombinatsii épiteliia i mezenkhimy émbrional'nykh legkikh ot intaktnykh i poluchavshikh hantserogen mysheĭ.