Effects of DDT on muscarine- and nicotine-like binding sites in CNS of immature and adult mice.
Immature (10 days of age) and adult (60 days of age) mice were given a single peroral administration of 0.5 mg p,p'-DDT/kg body wt. or a fat emulsion vehicle. The mice were killed 24 h or 7 days post-treatment and the muscarine- and nicotine-like binding sites were measured in the cerebral cortex and in the cerebellum using [3H]quinuclidinyl benzilate ([3H]QNB) and [3H]alpha-bungarotoxin ([3H]alpha-BTX), respectively, as ligands. A significant decrease in the [3H]QNB binding to the muscarine-like receptors in the cerebral cortex was seen in the 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT)-treated adults after 7 days. The effects on the muscarine-like receptors appeared to be different in the immature mice compared to the adults, showing an increase between 24 h and 7 days post-treatment in the immature and a decrease in the adults. No change was seen on the nicotine-like receptors.
D000367:Age Factors; D000818:Animals; D001921:Brain / Q000187:drug effects*; D002038:Bungarotoxins / Q000378:metabolism; D003634:DDT / Q000633:toxicity*; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000187:drug effects*; D011976:Receptors, Muscarinic / Q000187:drug effects; D011978:Receptors, Nicotinic / Q000187:drug effects
D002038:Bungarotoxins; D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D011978:Receptors, Nicotinic; D011813:Quinuclidinyl Benzilate; D003634:DDT