[The origin of 5 S DNA from tumor ascitic fluid].
Certain properties of DNA from chromatin mononucleosomes of the normal rat liver were compared with those of 5 S DNA from the ascitic fluids of human and animal tumours. Both DNA proved to be identical by their electrophoretic mobility in 8% PAAG and by characteristic violet tinge when stained with methylene blue. Thermal and alkaline denaturation causes less degradation of mononuclesome DNA than 5 S DNA. It is shown that 5 S DNA of ascitic fluid of Ehrlich ascite tumour of mice hybridizes with a higher amount of mouse genome DNA restricts. An assumption is advanced that 5 S DNA is either a product of chromatin decay to mononucleosomes or a product of extracopial synthesis and is delivered to ascitic fluid surrounding the tumour cells as a result of activation of Ca, Mg-dependent endonuclease of the latter.
D000818:Animals; D001202:Ascitic Fluid / Q000378:metabolism*; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008812:Mice, Hairless; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization
D004273:DNA, Neoplasm
Proiskhozhdenie 5 S DNK astsitnoĭ zhidkosti opukholeĭ.