title: Reversible denaturation of the estrogen receptor and estimation of polypeptide chain molecular weight.
abstract: The estrogen receptor protein loses its ability to bind to estrogens upon denaturation with sodium dodecyl sulfate and 2-mercaptoethanol. Binding activity is recovered at 60-80% efficiency upon removal of the denaturants, equilibration with 6 M guanidine hydrochloride, and dilution into buffer containing estrogen. Renatured receptor is similar to native receptor in affinity for 17 beta-estradiol and ability to bind DNA. Detection of receptor activity after sodium dodecyl sulfate-polyacrylamide gel electrophoresis of uterine or pituitary cytosol or of uterine nuclear extracts reveals a single unique polypeptide species of 65,000 mol wt.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004586:Electrophoresis; D005260:Female; D008722:Methods; D008970:Molecular Weight; D010902:Pituitary Gland / Q000378:metabolism; D011489:Protein Denaturation; D051381:Rats; D011919:Rats, Inbred Strains; D011960:Receptors, Estrogen / Q000302:isolation & purification*; D014599:Uterus / Q000378:metabolism
chemical_list: D011960:Receptors, Estrogen