Is cerebellar granule cell migration regulated by an internal clock?
We have studied the time course of migratory behavior of cerebellar granule cells in the microwell tissue culture system. [3H]Thymidine served as a marker for particular granule cell generations. When cultured 4 hr after [3H]thymidine injection for 6 days in microwell cultures, labeled granule cells were seen to migrate along fiber bundles expanding between reaggregates called "cables" for 3 to 4 days. After 5 and 6 days in vitro the percentage of labeled non-migrating cells found in clusters in reaggregates and on cables increased considerably. Whereas unlabeled cells continued to migrate. Comparable results were obtained when granule cells developed in vivo for various times after label and their developmental state was determined in vitro. Cells from cerebellar populations labeled 1 to 4 days before culture maintained their ability to migrate in vitro, even after granule cells had entered the internal granule cell layer. In contrast, the percentage of migrating cells labeled 5 and 6 days before culture was reduced significantly. The results suggest that the time span of granule cell migration is predetermined intrinsically rather than by external signals.
D000818:Animals; D001683:Biological Clocks; D002455:Cell Division; D002465:Cell Movement; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology; D013936:Thymidine; D014316:Tritium
D014316:Tritium; D013936:Thymidine