Anaerobic expression of maize fructose-1,6-diphosphate aldolase.
The anaerobic proteins of maize are a set of 10 major and 10 minor polypeptides selectively synthesized in anaerobic seedling roots. 1) Anaerobiosis resulted in the selected labeling of a protein which bound to Blue Sepharose and was eluted by fructose 1,6-diphosphate. 2) This protein elicited antiserum which recognized a single protein with molecular weight of approximately 40,000. 3) By Western blot analysis, this antiserum recognized a maize fructose-1,6-diphosphate aldolase purified to homogeneity. We show that two major anaerobic proteins of maize, ANP35.5 and ANP33A, correspond to a cytoplasmic fructose-1,6-diphosphate aldolase.
D000693:Anaerobiosis; D005634:Fructose-Bisphosphate Aldolase / Q000096:biosynthesis* / Q000302:isolation & purification; D010940:Plant Proteins / Q000302:isolation & purification; D010944:Plants / Q000201:enzymology*; D012685:Sepharose / Q000031:analogs & derivatives; D003313:Zea mays / Q000201:enzymology
D010940:Plant Proteins; C029483:Blue-Sepharose; D012685:Sepharose; D005634:Fructose-Bisphosphate Aldolase