Transmembrane potential of liver mitochondria from hexachlorobenzene-and iron-treated rats.
The respiratory parameters and the membrane potential of liver mitochondria from rats treated with either hexachlorobenzene, iron or hexachlorobenzene plus iron, to induce experimental porphyria, have been studied. Partial uncoupling of oxidative phosphorylation has been observed in mitochondria from hexachlorobenzene- and hexachlorobenzene plus iron-treated rats. Direct evidence has been presented that this uncoupling is due to the action of pentachlorophenol endogenously formed by metabolism of hexachlorobenzene. No irreversible damage of mitochondria membrane has been revealed under both these conditions. Normal oxidative phosphorylation has been found in mitochondria from rats treated with iron alone. In contrast, they presented an anomalous membrane potential, fully restored by oligomycin. A possible involvement of lipid peroxidation process, induced by iron, in causing these abnormalities has been suggested.
D000818:Animals; D002722:Chlorobenzenes / Q000494:pharmacology*; D005260:Female; D006581:Hexachlorobenzene / Q000494:pharmacology*; D007501:Iron / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000032:analysis / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects; D010416:Pentachlorophenol / Q000032:analysis; D011166:Porphyrins / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D002722:Chlorobenzenes; D011166:Porphyrins; D006581:Hexachlorobenzene; D010416:Pentachlorophenol; D007501:Iron