title: Effect of rumen-protected methionine on plasma free amino acids and production by dairy cows.
abstract: The effectiveness of an encapsulated rumen-protected methionine compound (RPMet) for delivering methionine postruminally and its effect on plasma amino acid concentrations, milk production and milk composition were evaluated. Thirty multiparous Holstein cows in early lactation were randomly assigned to five levels of RPMet. RPMet was supplied at 0, 550, 1100, 1650 or 3300 mg/kg of concentrate resulting in an intake of 0, 6.9, 12.9, 19.6 or 40.2 g of supplemental DL-methionine/day for each cow. The basal diet was alfalfa-bromegrass hay and a 15.8% crude protein corn- and soybean meal-based concentrate mixture. The experiment was divided into pretreatment and treatment periods, each consisting of 21 days. Feed intake and milk production were measured daily, milk composition weekly and plasma amino acid concentrations twice daily on days 17 and 20 of the pretreatment period and on days 4, 10 and 21 of the treatment period. RPMet was effective in delivering methionine postruminally, which increased concentrations of methionine in plasma. Dry matter intake, milk production, milk composition and body weight were not significantly affected by RPMet.
mesh_terms: D000596:Amino Acids / Q000008:administration & dosage / Q000097:blood*; D000821:Animal Feed; D000818:Animals; D002417:Cattle; D005260:Female; D008715:Methionine / Q000097:blood / Q000378:metabolism*; D008892:Milk / Q000032:analysis; D012417:Rumen / Q000502:physiology*
chemical_list: D000596:Amino Acids; D008715:Methionine