Radioimmunoassay for the amino-terminal sequences of type III procollagen in human body fluids measuring fragmented precursor sequences.
Antisera specifically directed against the amino-terminal precursor sequence of bovine p-N-collagen type III (Col 1-3 III) have been raised in rabbit. When tested in a radioimmunoassay, parallel displacement curves and complete cross-reactivity were observed between bovine and human purified Col 1-3 III. However, these displacement curves were not parallel to that obtained with serially diluted human sera which presented a smoother slope. Cleavage of the Col 1-3 III peptide by collagenase at high temperature yielded an immunoreactive preparation containing two smaller peptides (Col 1 plus Col 2) III. The displacement curves obtained with human or bovine (Col 1 plus Col 2) preparations were parallel to those obtained with serially diluted human sera or other human body fluids (amniotic and ascitic fluids). Quantitative measurements of the circulating antigen in normal and pathological conditions are possible only when the (Col 1 plus Col 2) is used as standard antigen. This radioimmunoassay represents an original and simple technique offering good accuracy. The amount of (Col 1 plus Col 2) III in human adults is 69.1 +/- 27.7 ng/ml, with a distribution of frequency close to the normal. It is increased in liver cirrhosis and other conditions characterized by an active neoformation of connective tissue.
D000328:Adult; D000595:Amino Acid Sequence; D000653:Amniotic Fluid / Q000032:analysis; D000818:Animals; D001202:Ascitic Fluid / Q000378:metabolism; D002417:Cattle; D003429:Cross Reactions; D005260:Female; D005312:Fetal Blood / Q000032:analysis; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis; D011247:Pregnancy; D011347:Procollagen / Q000032:analysis* / Q000097:blood / Q000652:urine; D011863:Radioimmunoassay
D010446:Peptide Fragments; D011347:Procollagen