Determination of 3 methyl-L-histidine in human urine by ion exchange high performance liquid chromatography. Applications to patients in post-operative surgical care.
A new chromatographic procedure is proposed for measuring 3 methyl-L-histidine (3 MH) in human urine. The sample was purified on a cation-exchange resin (AGR 50W-X4) and analysed by ion exchange high performance liquid chromatography on a PARTISIL 10 SCX Whatman column in UV light at 210 nm within 16 min. This procedure gave similar outputs of 3 MH to those described in human normal urine (mean +/- SEM = 213 +/- 15 mumol X 24 h-1, n = 19). It was used to measure the urinary outputs of 3 MH of five patients admitted to an intensive surgical care unit, for 48, 28, 25, 15 and 10 days, respectively. The urinary outputs of 3 MH were normal or lower than normal. The 10(3) urinary 3 MH/creatinine molar ratios were also calculated; this new 3 MH analysis could help the reanimator to prescribe an adequate nutritional assessment.
D000293:Adolescent; D000328:Adult; D001930:Brain Injuries / Q000601:surgery / Q000652:urine; D002481:Cellulitis / Q000601:surgery / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D003404:Creatinine / Q000652:urine; D005260:Female; D006639:Histidine / Q000031:analogs & derivatives*; D006801:Humans; D008297:Male; D008762:Methylhistidines / Q000652:urine*; D008875:Middle Aged; D010538:Peritonitis / Q000601:surgery / Q000652:urine; D011182:Postoperative Care; D012253:Rib Fractures / Q000601:surgery / Q000652:urine
D008762:Methylhistidines; D006639:Histidine; D003404:Creatinine; C028118:3-methylhistidine