title: Use of t-butyldimethylsilylation in the gas chromatographic/mass spectrometric analysis of physiologic compounds found in plasma using electron-impact ionization.
abstract: The use of N-methyl-N-(t-butyldimethylsilyl)trifluoroacetamide to prepare the t-butyldimethylsilyl derivatives of a number of organic compounds (selected amino acids, alpha-keto acids, ketone bodies, free fatty acids, urea, glycerol, lactate, and pyruvate) is reported. These derivatives are particularly useful for gas chromatographic/mass spectrometric analysis involving the use of stable isotopes and selected ion monitoring, since a peak of sufficient abundance at 57 mass/charge units below the molecular ion was always present, and was the result of the loss of one t-butyl group. In each case, this fragment contained the entire skeleton of the original compound, which permitted easy analysis using electron-impact ionization of these compounds alone or when labeled with stable isotopes in any nonexchangeable position.
mesh_terms: D000596:Amino Acids / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry; D005990:Glycerol / Q000097:blood; D007651:Keto Acids / Q000097:blood; D007657:Ketone Bodies / Q000097:blood; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D017646:Organosilicon Compounds; D011773:Pyruvates / Q000097:blood; D012825:Silicon; D014508:Urea / Q000097:blood
chemical_list: D000596:Amino Acids; D005230:Fatty Acids, Nonesterified; D007651:Keto Acids; D007657:Ketone Bodies; D007773:Lactates; D017646:Organosilicon Compounds; D011773:Pyruvates; C009660:t-butyldimethylsilyl compounds; D019344:Lactic Acid; D014508:Urea; D005990:Glycerol; D012825:Silicon