Fluorometric measurement of tin-protoporphyrin in biological samples.
Sn-protoporphyrin is a potent competitive inhibitor of heme oxygenase, can suppress neonatal and other forms of hyperbilirubinemia in laboratory animals, and represents a potential new approach to the treatment of neonatal jaundice in humans. In order to study the disposition of Sn-protoporphyrin in vivo we have developed a sensitive fluorometric method for the quantitation of this metalloporphyrin in biological samples. The method is sensitive to concentrations as low as 0.01 nmol/ml, and is specific for Sn-protoporphyrin even in the presence of other porphyrins such as protoporphyrin.
D000818:Animals; D005243:Feces / Q000032:analysis; D006801:Humans; D008297:Male; D008665:Metalloporphyrins; D011166:Porphyrins / Q000032:analysis*; D011524:Protoporphyrins / Q000032:analysis* / Q000097:blood / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains; D012996:Solutions; D013050:Spectrometry, Fluorescence
D008665:Metalloporphyrins; D011166:Porphyrins; D011524:Protoporphyrins; D012996:Solutions; C032628:tin protoporphyrin IX