title: Cross-linking of histone H5 in hen erythrocyte nuclei.
abstract: Following treatment of hen erythrocyte nuclei with dimethyl 3,3'-dithiobispropionimidate, dimers between histones H1a, H1b, and H5 were extracted with 5% perchloric acid. They resolved electrophoretically into four sub-bands and these were identified by non-reducing/reducing gel electrophoresis. The H5-H5 homodimer species was purified by gel electrophoresis and was treated sequentially with BrCN and dithiothreitol. The pattern of resulting fragments indicates that cross-links were mainly formed between the COOH-terminal portions and at a significantly lower frequency between the COOH-terminal and the NH2-terminal portions.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002645:Chickens; D003432:Cross-Linking Reagents / Q000494:pharmacology; D003488:Cyanogen Bromide / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects / Q000648:ultrastructure*; D005260:Female; D006657:Histones / Q000378:metabolism*; D007096:Imidoesters / Q000494:pharmacology; D046911:Macromolecular Substances
chemical_list: D003432:Cross-Linking Reagents; D006657:Histones; D007096:Imidoesters; D046911:Macromolecular Substances; C007471:dimethyl dithiobispropionimidate; D003488:Cyanogen Bromide