Specific mediation of cellular immunity to Toxoplasma gondii in somatic cells of mice.
Lymphocytes from mice immunized against Toxoplasma gondii protected T. gondii-infected macrophage and kidney cell cultures. After contact with antigens, supernatants of such immune lymphocytes, also contained a factor protective for T. gondii-infected macrophages and kidney cells. Supernatants were protective only when the lymphocytes and kidneys cells were isogeneic. Protection was specific in that supernatants from only T. gondii-immune, but not Besnoitia jellisoni-immune, lymphocytes provided protection against toxoplasmosis. Sixteen to 24 h were required for an appreciable amount of protective factor to be secreted; a similar absorption time was necessary for kidney cells to be protected. Peritoneal lymphocyte lysates, prepared as transfer factor, contained protective substances with a potency similar to that of lymphocyte supernatants, which were also strain restricted in their effect.
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D005260:Female; D007111:Immunity, Cellular; D007114:Immunization; D007668:Kidney / Q000276:immunology / Q000469:parasitology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000276:immunology*
D000953:Antigens, Protozoan