[Method of disinfecting plague-infected fleas in preparation for electron microscopic study].
During fixation of fleas infected with plague agent (with preliminary cut heads, limbs and posterior part of the abdomen) in 2.5% glutaric dialdehyde or 2% osmium tetroxide their disinfection was obtained in two days. After double fixation with 2-hour exposure in each of these fixators disinfection of the material was acheived only after additional 2-day maintenance in 70% ethyl alcohol. Fleas, which had been placed into fixators or in 70% ethyl alcohol after double fixation without preliminary dissection, were disinfected completely only in 10 days.
D000818:Animals; D004202:Disinfectants / Q000494:pharmacology; D004203:Disinfection / Q000379:methods*; D008854:Microscopy, Electron; D005423:Siphonaptera / Q000382:microbiology* / Q000648:ultrastructure; D013242:Sterilization / Q000379:methods*; D013997:Time Factors; D015010:Yersinia pestis / Q000187:drug effects*
D004202:Disinfectants
K metodike obezzarazhivaniia infitsirovannykh vozbuditelem chumy blokh pri podgotovke k élektronno-mikroskopicheskomu issledovaniiu.