Picosecond absorption studies on rhodopsin and isorhodopsin in detergent and native membrane.
Picosecond transient absorption spectra of rhodopsin and isorhodopsin were measured at room temperature with a double-beam laser spectrophotometer after excitation at 355 nm. Photolysis studies were performed on rhodopsin solubilized in two different detergents (digitonin and 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate). The resulting rhodopsin/bathorhodopsin absorption difference spectra were measured at times from 35 ps to 250 ns following photoexcitation. Rhodopsin and isorhodopsin in native disk membrane were studied after suspension in 75% glycerol. Isorhodopsin was prepared by photoisomerizing rhodopsin in disk membrane at 77 K. Transient spectra obtained from the visual pigments in native membrane were of a quality approaching that obtained from detergent-solubilized rhodopsin. The batho intermediate derived from isorhodopsin was spectrally the same as that generated by rhodopsin photolysis and was produced with a quantum yield higher than had been predicted on the basis of other studies.
D000818:Animals; D002417:Cattle; D002462:Cell Membrane; D002793:Cholic Acids; D004072:Digitonin; D007536:Isomerism; D008027:Light; D010782:Photolysis; D012168:Retinal Pigments / Q000528:radiation effects*; D012243:Rhodopsin / Q000528:radiation effects*; D012374:Rod Cell Outer Segment / Q000032:analysis; D012995:Solubility; D013053:Spectrophotometry
D002793:Cholic Acids; D012168:Retinal Pigments; C042882:isorhodopsin; D012243:Rhodopsin; D004072:Digitonin; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate