Entrance of cholera enterotoxin subunits into thymus cells.
Analysis of the staining of cholera enterotoxin on the surface of cells with specific antibodies against each subunit of cholera enterotoxin, using a fluorescence-activated cell sorter and electron microscopy, showed that not only subunit A but also subunit B penetrates the cell membrane. The detection of subunits inside the cell was facilitated by the use of saponin, an agent that increases membrane permeability.
D000818:Animals; D002771:Cholera; D004768:Enterotoxins / Q000378:metabolism*; D006651:Histocytochemistry; D051379:Mice; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D012503:Saponins; D013950:Thymus Gland / Q000378:metabolism* / Q000648:ultrastructure
D004768:Enterotoxins; D012503:Saponins; C037420:stN protein, Vibrio cholerae