Thin-layer chromatography of hyaluronate oligosaccharides.
Odd- and even-numbered hyaluronate oligosaccharides with N-acetylglucosamine, glucuronic acid, or 4,5-unsaturated glucuronic acid at their nonreducing ends were separated by thin-layer chromatography, using silica gel and a solvent system of isopropanol-water (66 : 34) containing 0.05 M NaCl. In the isopropanol system, small amounts of electrolytes were necessary for the resolution of each oligosaccharide.
D000117:Acetylglucosamine / Q000032:analysis; D002855:Chromatography, Thin Layer / Q000379:methods; D005260:Female; D005965:Glucuronates / Q000032:analysis; D020723:Glucuronic Acid; D006801:Humans; D006820:Hyaluronic Acid / Q000302:isolation & purification; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D008297:Male; D009844:Oligosaccharides / Q000302:isolation & purification*; D011247:Pregnancy; D013302:Streptomyces / Q000201:enzymology; D013329:Structure-Activity Relationship; D013737:Testis / Q000201:enzymology; D014470:Umbilical Cord
D005965:Glucuronates; D009844:Oligosaccharides; D020723:Glucuronic Acid; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase; D000117:Acetylglucosamine