title: The influence of differing connective tissue substrates on the maintenance of adult stratified squamous epithelia.
abstract: The interaction between adult stratified squamous epithelium and its supporting connective tissue possibly involves both permissive and directive influences. To examine the effect of vitality and specificity of connective tissue on the maintenance of epithelial structure and histodifferentiation, specimens of skin and oral mucosa from various regions of adult mice were separated using either EDTA or trypsin. Prior to transplantation, the epithelium was recombined with either inverted homologous connective tissue or with connective tissue that had been killed either by heating or repeated freeze-thawing. Epithelial sheets were also transplanted onto the graft bed alone or in combination with striated muscle or tendon. Normal patterns of cytodifferentiation were maintained when the epithelium was recombined with inverted or frozen-thawed subepithelial connective tissue but there was a loss of spatial organization on the frozen-thawed connective tissue. In contrast, heat-killed or trypsin-treated frozen-thawed subepithelial connective tissue and non-dermal connective tissue failed to maintain a viable epithelium. These observations suggest that subepithelial connective tissues (dermis, lamina propria) but not deep connective tissues facilitate epithelial proliferation and histodifferentiation.
mesh_terms: D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D003239:Connective Tissue Cells; D004847:Epithelial Cells; D004848:Epithelium / Q000637:transplantation; D005615:Freezing; D051379:Mice; D008815:Mice, Inbred Strains; D009928:Organ Specificity; D014021:Tissue Preservation