[Characteristics of micromethods for the analysis of the in vitro synthesis of antibodies against xenogenic erythrocytes].
In order to choose an optimal assay for screening cell microcultures containing anti-xenogenic erythrocyte antibodies in their supernatants, two microassays were compared: microhemagglutination and hemolytic patch assay. Samples of murine sera, each in both assays, were examined for the antibody titer. It is shown that the sensitivity of the assays is approximately at the same level, as the statistically significant difference in the amount of antibodies evaluated either by hemagglutination or by patch assay was not found. Hence one should not prefer any of the assays from the standpoint of sensitivity, when choosing the screening procedure for hybridomas of lymphoid cell microcultures secreting antibodies against xenogenic red blood cells.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000917:Antibody Formation; D003168:Complement Fixation Tests; D004912:Erythrocytes / Q000276:immunology*; D006386:Hemagglutination Tests; D007114:Immunization; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012756:Sheep
D000906:Antibodies
Kharakteristika mikrometodov issledovaniia sinteza in vitro antitel k ksenogennym éritrotsitam.