Epidermal benzo[a]pyrene metabolism and DNA-binding in Balb/C mice: inhibition by ellagic acid.
Topical application of ellagic acid, a common plant phenol, to control or to 3-methylcholanthrene (3-MC) pretreated Balb/C mice, resulted in significant inhibition of hepatic and epidermal microsomal aryl hydrogen hydroxylase activity, and of benzo[a]pyrene (BP) binding to epidermal and hepatic DNA in vivo. In vitro addition of ellagic acid (0.25 mM) to epidermal microsomal incubation systems from either control or 3-MC-treated animals resulted in 62-75% inhibition of BP binding to calf thymus DNA. These studies suggest that ellagic acid could prove useful in understanding and/or modulating polyaromatic hydrocarbon carcinogenesis.
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001189:Aryl Hydrocarbon Hydroxylases / Q000037:antagonists & inhibitors; D001564:Benzo(a)pyrene / Q000378:metabolism*; D001578:Benzopyrans / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism*; D004610:Ellagic Acid / Q000494:pharmacology*; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C; D008861:Microsomes / Q000378:metabolism
D001578:Benzopyrans; D004610:Ellagic Acid; D001564:Benzo(a)pyrene; D008748:Methylcholanthrene; D004247:DNA; D001189:Aryl Hydrocarbon Hydroxylases