alpha-Alkyl-alpha-arylacetic acid derivatives as fluorescence markers for thin-layer chromatographic and high-performance liquid chromatographic assay of amines and alcohols.
Activated R,S-benoxaprofen is described as a new reagent for fluorescent derivatization of drugs with primary or secondary amino groups or with hydroxyl groups. Separation of the reaction products is demonstrated by thin-layer chromatography and high-performance liquid chromatography. The sensitivity of the detection is in the picomole range. Derivatization procedures can be easily and rapidly performed.
D000388:Air; D000438:Alcohols / Q000032:analysis*; D000588:Amines / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002794:Choline / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004355:Drug Stability; D005456:Fluorescent Dyes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D000432:Methanol / Q000032:analysis; D010627:Phenethylamines / Q000032:analysis; D011422:Propionates / Q000032:analysis; D012997:Solvents; D014191:Tranylcypromine / Q000032:analysis
D000438:Alcohols; D000588:Amines; D005456:Fluorescent Dyes; D010627:Phenethylamines; D011422:Propionates; D012997:Solvents; C011677:benoxaprofen; D014191:Tranylcypromine; C033198:1-phenethylamine; D002794:Choline; D000432:Methanol