Reaction of vitamin K and dithiothreitol on reversed-phase C18 high-performance liquid chromatographic columns.
A non-enzymatically catalyzed side reaction between dithiothreitol and vitamin K occurs on reversed-phase C18 high-performance liquid chromatographic columns. Excess dithiothreitol may be effectively removed from enzymatic reaction mixtures with N-ethylmaleimide. A low polarity gradient elution system is reported which gives quantitative recoveries (greater than 95%) of the vitamins in a concentration range useful for analysis of enzymatically catalyzed reactions.
D002725:Chloroform; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004229:Dithiothreitol / Q000032:analysis*; D005033:Ethylmaleimide; D006586:Hexanes; D014812:Vitamin K / Q000032:analysis*
D006586:Hexanes; D014812:Vitamin K; D002725:Chloroform; D005033:Ethylmaleimide; D004229:Dithiothreitol