title: Investigation of two fluorinated reagents for the analysis of selenium by gas chromatography.
abstract: As potential reagents for the determination of traces of selenium by gas chromatography, the compounds 4-fluoro-o-phenylenediamine and 4-trifluoromethyl-o-phenylenediamine have been examined and compared with seven o-diamines previously reported for analytical purposes. Retention times of the fluoro-piaselenols are shorter than all other compounds but, with the electron-capture detector, their responses differ widely. However, the detection limit of the trifluoromethylpiaselenol compares favourably with the commonly used derivative, 5-nitropiaselenol. The analytical requirements of 4-trifluoro-o-phenylenediamine as a reagent, and the results of its application to the determination of selenium in various biological matrices, are presented.
mesh_terms: D000818:Animals; D001393:Azoles / Q000138:chemical synthesis; D001826:Body Fluids / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D003959:Diamines / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008892:Milk / Q000032:analysis; D016566:Organoselenium Compounds; D010944:Plants / Q000032:analysis; D012643:Selenium / Q000032:analysis* / Q000138:chemical synthesis / Q000378:metabolism; D013696:Temperature
chemical_list: D001393:Azoles; D003959:Diamines; D007202:Indicators and Reagents; D016566:Organoselenium Compounds; C042817:piaselenole; D012643:Selenium