Mechanism of inhibition of human natural killer activity by ultraviolet radiation.
Ultraviolet radiation (UVR) has been shown to inhibit various immune functions in vivo and in vitro. We have confirmed that UVR inhibits human natural killer (NK) activity in vitro and have shown that UVR inhibits human ADCC. In this report, the mechanism by which UVR inhibits NK function was investigated by analyzing the stage at which the inhibiting activity occurs and the ability of the NK cells to release cytotoxic factors previously shown to be involved in CMC. Single cell assays in Agarose revealed that inhibition of NK activity was localized at the postbinding lethal hit stage rather than the initial recognition or binding stage of lysis. We then examined whether UV-treated cells were able to release cytotoxic factors after stimulation with target cells. As expected, stimulated cells released cytotoxic factors, yet, surprisingly, these factors were also released in the absence of stimulator cells. The spontaneous release was detectable in the supernatants as early as 30 min after UV irradiation. The lytic material examined in 48- to 72-hr viability assays was not NK specific, because lysis was obtained with a wide range of NK sensitive and resistant target cells. These results demonstrate that UVR does not alter the capacity of the cells to secrete cytotoxic material, but in fact enhances its release. Several possible mechanisms are proposed to explain the UVR-induced NK inhibition.
D000920:Antibody-Dependent Cell Cytotoxicity / Q000528:radiation effects; D002460:Cell Line; D003602:Cytotoxicity, Immunologic / Q000528:radiation effects*; D006801:Humans; D007165:Immunosuppression Therapy; D007694:Killer Cells, Natural / Q000276:immunology / Q000528:radiation effects*; D055646:Killer Factors, Yeast; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects; D014176:Protein Biosynthesis; D011506:Proteins; D014466:Ultraviolet Rays
D055646:Killer Factors, Yeast; D011506:Proteins