The effect of NPT 15392, 9-(erythro-2-hydroxy-3-nonyl)-6-hydroxypurine, on the phytohemagglutinin of OKT3+, OKT4+, OKT8+, and OKM1+ cell-depleted and undepleted peripheral blood mononuclear cells.
NPT 15392, 9-(erythro-2-hydroxy-3-nonyl)-6-hydroxypurine, has been reported to influence immunological responses involving different cell types. Herein data are obtained by studying the influence of NPT 15392 on the phytohemagglutinin (PHA)-induced proliferative responses of unfractionated peripheral blood lymphocytes (PBLs) and of cell suspensions, which have been depleted of the cell subsets recognized by the monoclonal antibodies OKT3, OKT4, OKT8, and OKM1, in an attempt to identify which cell types respond to NPT 15392 in the PHA-driven blast transformation assay. The proliferative responses of unfractionated peripheral blood lymphocytes are potentiated when the drug is employed at the concentration of 0.1 microgram/ml and inhibited when NPT 15392 is added to the cell suspensions at concentrations over 5 micrograms/ml. The data reported here suggest that this phenomenon is a composite effect, made up of a combination of the counteracting effects caused by the OKT4+ cells on the one hand, and the OKT8+ and OKM1+ cells on the other.
D000911:Antibodies, Monoclonal / Q000276:immunology; D006801:Humans; D007042:Hypoxanthines / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008212:Lymphocyte Depletion; D008214:Lymphocytes / Q000145:classification; D010835:Phytohemagglutinins / Q000276:immunology
D000911:Antibodies, Monoclonal; D007042:Hypoxanthines; D010835:Phytohemagglutinins; C026533:NPT 15392