Acidification of interstitial fluid in hippocampal formation caused by seizures and by spreading depression.
Changes in the pH of interstitial fluid were measured with H+-selective double-barreled micropipette electrodes in fascia dentata of urethane-anesthetized rats. Paroxysmal afterdischarges provoked by repetitive stimulation of an afferent fiber tract brought in their wake acidification by 0.07 to 0.2 pH units. Spreading depression caused acidification by 0.2-0.5 pH units. Acid shifts were often preceded by transient alkalinization. Acidification is attributed to the production of CO2 and of other acid metabolites.
D000818:Animals; D013181:Cortical Spreading Depression; D004558:Electric Stimulation; D005073:Evoked Potentials, Somatosensory; D005110:Extracellular Space / Q000502:physiology*; D006624:Hippocampus / Q000502:physiology* / Q000503:physiopathology; D006863:Hydrogen-Ion Concentration; D008297:Male; D051381:Rats; D012640:Seizures / Q000503:physiopathology*