Generation of slow-reacting substance (leukotrienes) by endotoxin and lipid A from human polymorphonuclear granulocytes.
Leukotrienes were released from human polymorphonuclear granulocytes on incubation with endotoxins and lipid A. The analysis was performed by their smooth muscle contracting properties, reversed phase high-pressure liquid chromatography and radioimmunoassay for leukotrienes C4 and D4. The active component of the lipopolysaccharides seems to be the lipid A portion.
D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D004731:Endotoxins / Q000494:pharmacology*; D006801:Humans; D008050:Lipid A / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D013189:SRS-A / Q000032:analysis / Q000096:biosynthesis*; D012475:Salmonella
D004731:Endotoxins; D008050:Lipid A; D013189:SRS-A