Quantitative analysis and pattern recognition of two-dimensional electrophoresis gels.
We describe a system, both hardware and software, that provides quantitative analysis and data reduction of two-dimensional electrophoresis gels. Image-analysis techniques are used to determine spot intensities and to match spot patterns among many gels. A pattern-recognition program is used to extract the useful information contained in the spot lists. The application of this technology to a study of supernates from bacterial cultures is described.
D001426:Bacterial Proteins / Q000032:analysis*; D001330:Electronic Data Processing / Q000379:methods*; D004586:Electrophoresis / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D013045:Species Specificity; D013211:Staphylococcus aureus
D001426:Bacterial Proteins