title: Structural and functional alterations caused at the extraneuronal level by sympathetic denervation of blood vessels.
abstract: The lateral saphenous vein of the dog and the rabbit ear artery were surgically denervated, by clamping the vessel or by removal of the superior cervical ganglion, respectively. Both procedures resulted in denervation of the vessels. The denervated, lateral saphenous vein was supersensitive to exogenous noradrenaline and inactivation of the amine (in oil immersion experiments) was slower in denervated vein strips than in control strips treated with cocaine. Incubation experiments with 3H-noradrenaline confirmed that denervated strips formed considerably fewer metabolites than control ones (in the absence or presence of cocaine) and that O-methylation of noradrenaline was reduced by about 50%. When the strips were incubated with 3H-isoprenaline, the denervated ones accumulated and metabolized isoprenaline to a lesser degree than control strips. Hydrocortisone did not reduce the accumulation of isoprenaline in the denervated vein and had only minor effects on O-methylation. The metabolism of noradrenaline and isoprenaline gradually recovered with time. In the ear artery, denervation was accompanied by a marked reduction in O-methylation, but not in accumulation, of isoprenaline. In both vessels there was a highly significant positive correlation between noradrenaline content and O-methylating capacity; in the saphenous vein accumulation of isoprenaline was also positively correlated to noradrenaline content. Morphological changes observed in the denervated vessels consisted essentially in dedifferentiation of smooth muscle cells (which attained larger dimensions, had an indented, large nucleus, augmented euchromatin and an increased amount of ribosomes), abundance of extracellular material and fibroblasts.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D001158:Arteries / Q000294:innervation / Q000378:metabolism / Q000648:ultrastructure; D004285:Dogs; D004423:Ear / Q000098:blood supply*; D006632:Histamine / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology; D007545:Isoproterenol / Q000378:metabolism; D008745:Methylation; D009131:Muscle, Smooth, Vascular / Q000648:ultrastructure; D009638:Norepinephrine / Q000378:metabolism; D011817:Rabbits; D012110:Reserpine / Q000494:pharmacology; D012501:Saphenous Vein / Q000294:innervation* / Q000378:metabolism / Q000648:ultrastructure; D013562:Sympathectomy; D013564:Sympathetic Nervous System / Q000502:physiology*
chemical_list: D006632:Histamine; D012110:Reserpine; D007545:Isoproterenol; D006854:Hydrocortisone; D009638:Norepinephrine