[Determination of deoxynivalenol and nivalenol in cereals at the microgram/kilogram range].
A method is described for the determination of Deoxynivalenol (DON) and Nivalenol (NV). A liquid chromatographic clean-up step is followed by silylation of the extract and the trimethylsilylether are determined by gas-chromatography-mass-spectrometry (GC-MS), monitoring the characteristic ions in the negative chemical ionisation mode. 71% of 42 food-samples were contaminated with DON and 38% with NV. The highest contamination detected was 2000 micrograms/kg DON and 120 micrograms/kg NV in a wheat bran sample. Deoxynivalenol and Nivalenol in concentrations of 10 micrograms/kg can be detected easily by this method.
D002855:Chromatography, Thin Layer; D002523:Edible Grain / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D008970:Molecular Weight; D009183:Mycotoxins / Q000032:analysis*; D012717:Sesquiterpenes / Q000032:analysis*; D014255:Trichothecenes / Q000032:analysis*
D009183:Mycotoxins; D012717:Sesquiterpenes; D014255:Trichothecenes; C038405:nivalenol; C007262:deoxynivalenol
Untersuchung von Cerealien auf Deoxynivalenol und Nivalenol im unteren ug/kg-Bereich.