A radiochemical method for determination of ethanol oxidation.
A radiochemical method for the measurement of ethanol oxidation by tissue preparations is described. Ethanol oxidation is determined from the production of [14C]acetaldehyde, quantified as the semicarbazone derivative, from [1-14C]ethanol. The assay is quantitative, reproducible and highly correlated with the NADH-enzymic-spectrophotometric procedure.
D000818:Animals; D000431:Ethanol / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D010084:Oxidation-Reduction; D011854:Radiochemistry / Q000379:methods*; D051381:Rats; D013053:Spectrophotometry
D000431:Ethanol