The estrogenic activity of DDT: the in vitro induction of an estrogen-inducible protein by o,p'-DDT.
Induced protein is a commonly measured marker for estrogenic action. The induction of induced protein by o,p'-DDT was studied in an in vitro system. Nuclear levels of estrogen receptor translocated by o,p'-DDT correlated highly with induced protein induction, and the time course for induced protein induction was consistent with an estrogen receptor-mediated mechanism. While the maximum amount of induced protein produced by o,p'-DDT was less than after 17 beta-estradiol exposure, the induced protein formed by each compound was indistinguishable on nondenaturing and denaturing polyacrylamide gels. Also, it was shown that o,p'-DDT does not cause additional induction of induced protein over that seen with maximum levels of 17 beta-estradiol, further supporting the premise that these compounds share a common pathway in stimulating the synthesis of induced protein.
D000818:Animals; D003634:DDT / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004958:Estradiol / Q000494:pharmacology; D004968:Estrogens, Non-Steroidal; D005260:Female; D066298:In Vitro Techniques; D014176:Protein Biosynthesis; D051381:Rats; D011960:Receptors, Estrogen / Q000187:drug effects; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
D004968:Estrogens, Non-Steroidal; D011960:Receptors, Estrogen; D004958:Estradiol; D003634:DDT; C016340:o,p'-DDT