Relationship between the rate of erythrocyte hexose monophosphate pathway and the glucose 6-phosphate concentration.
Erythrocytes of individuals with increased (+ 50%) or reduced (-35%) hexokinase activity contain respectively 70 and 17 nmole/ml RBC of glucose-6-phosphate (normal concentration 30 +/- 5nmole/ml RBC) and show comparable rates of the HMP (60 +/- 5nmole/hr/ml RBC). Similarly, in RBC of different ages, obtained by density gradient ultracentrifugation, the glucose-6-phosphate concentration range from 57 (young cells) to 18 (old cells) nmole/ml RBC but the rate at which glucose is utilized in the HMP is unchanged. These data exclude a regulatory role of glucose 6-phosphate in the HMP even if its concentration is under that required for maximal G6PD activity.
D002906:Chromosomes, Human, 6-12 and X; D004912:Erythrocytes / Q000201:enzymology*; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000097:blood*; D006579:Heterozygote; D006593:Hexokinase / Q000097:blood / Q000172:deficiency; D006801:Humans; D009249:NADP / Q000097:blood; D010427:Pentose Phosphate Pathway; D014314:Trisomy
D005958:Glucosephosphates; D009249:NADP; D019298:Glucose-6-Phosphate; D006593:Hexokinase