A micelle model for the sedimentation behavior of bovine beta-casein.
The monomer-single polymer model of G.A. Gilbert (Disc. Faraday Soc. 20 (1955) 68) for moving boundary sedimentation has been used by Payens and colleagues to explain the observed results for bovine caseins, and by Harrington and colleagues to explain the observed results for myosin fibrils. Electron microscope pictures of Buchheim and Schmidt have subsequently revealed micellar beta-casein in the form of slightly elongated or spherical particles having a bimodal size distribution, but with a broad range of particle sizes, at concentrations not too far above the critical micelle concentration. The equilibrium properties of a broadly distributed micellar system can be fitted by the shell model developed by one of us, and in the present article, the shell model is extended to predict the moving boundary sedimentation behavior of such a system. The observed sedimentation patterns, as well as the critical concentration predictions of the monomer-single polymer Gilbert sedimentation model, are satisfactorily described with the present model, based on a continuous distribution of intermediates between monomers and the largest possible spherical micelles. For one example considered, the predicted frequency distribution of molecular weight is in qualitative agreement with the frequency distribution of particle volume found by Buchheim and Schmidt.
D000818:Animals; D002364:Caseins; D002417:Cattle; D003102:Colloids; D046911:Macromolecular Substances; D008433:Mathematics; D008823:Micelles; D011487:Protein Conformation; D014461:Ultracentrifugation
D002364:Caseins; D003102:Colloids; D046911:Macromolecular Substances; D008823:Micelles