title: A monoclonal antibody to the double-stranded polyribonucleotide complex poly(A) X poly(U).
abstract: A monoclonal antibody to the double-stranded polyribonucleotide complex poly(A) . poly(U) was derived from the fusion of spleen cells from immunized DBA/2 mice and the P3 X X63-Ag8 plasma cytoma. Specificity studies using radioimmunoassays showed that the anti-poly(A) . poly(U) does not cross-react with single-stranded polyribonucleotides. RNA X DNA hybrids or DNAs. In addition to RNA duplexes associating adenine and uracil, it recognizes synthetic poly(I) . poly(C) and naturally occurring reovirus RNA. It is thus directed against a conformational epitope with an absolute requirement for two polyribose phosphate chains. However, the antibody does not cross-react with poly(G) . poly(C) and is therefore able to distinguish between RNA double helices.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D001667:Binding, Competitive; D002478:Cells, Cultured; D003429:Cross Reactions; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D008968:Molecular Conformation; D010954:Plasmacytoma / Q000276:immunology; D011063:Poly A-U / Q000276:immunology*; D011066:Poly C / Q000276:immunology; D011068:Poly G / Q000276:immunology; D011070:Poly I-C / Q000276:immunology; D011072:Poly U / Q000276:immunology; D012367:RNA, Viral / Q000276:immunology; D011863:Radioimmunoassay; D013154:Spleen / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D012367:RNA, Viral; D011063:Poly A-U; D011068:Poly G; C008961:poly G-poly C; D011072:Poly U; D011066:Poly C; D011070:Poly I-C