A new method for high-performance liquid chromatographic determination of drotaverine in plasma.
A sensitive, specific high-performance liquid chromatographic procedure was developed for the determination of plasma drotaverine levels. Basic plasma samples were adjusted to pH 1.5 and extracted with chloroform. HPLC [n-heptane-dichloromethane-diethylamine (50:25:2)] on a microporous silica column, with a variable-wavelength UV detector set at 302 nm allowed the measurement of drotaverine at the 50-ng/mL level. The utility of this method for determination of drotaverine in dog and rat plasma was demonstrated.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D010208:Papaverine / Q000031:analogs & derivatives* / Q000097:blood; D010276:Parasympatholytics / Q000097:blood*; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D010276:Parasympatholytics; C005317:drotaverin; D010208:Papaverine