title: On the occurrence of polymers of H1, H1(0) and H5 in extracts of whole tissues. Artificial production during protein preparation.
abstract: Inspection of preparations of H1, H1(0) and H5 histones made by extraction of whole tissues has shown that dimers and higher polymers of all three of these proteins are present. They may be formed by the cross-linking action of poly(ADP-ribose) chains which are linked to some of the protein molecules. Putative dimers and higher polymers were noted in preparations from various tissues and species. However, evidence is presented which suggests that production of these polymers is an artifact of precipitation during the preparation of the proteins, so the significance of the polymers is questionable.
mesh_terms: D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000032:analysis* / Q000378:metabolism; D008099:Liver / Q000032:analysis; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism; D011108:Polymers / Q000032:analysis*; D011817:Rabbits; D013552:Swine; D013950:Thymus Gland / Q000032:analysis; D014982:Xenopus laevis
chemical_list: D006657:Histones; D011108:Polymers; D011064:Poly Adenosine Diphosphate Ribose