TMAO-ase activity in tissues of fish species from the Northeast Atlantic.
The influence of various parameters (evacuation; pH value; addition of Fe2+, cysteine, ascorbic acid and methylene blue) on the activity of the trimethylamine oxide demethylase (TMAO-ase) in extracts from the kidney of saithe (Pollachius virens L.) is described. Assay conditions resulting in maximum formaldehyde (FA) production were used to measure the TMAO-ase activity in kidney, spleen, liver, pyloric caeca, blood and muscle from Northern Atlantic fish species. Only in organs from fishes of the order Gadiformes could TMAO-ase activity be detected. The TMAO-ase was most active in kidney and spleen whereas in muscle the enzyme activity could be barely detected.
D000818:Animals; D001274:Atmospheric Pressure; D002432:Cecum / Q000201:enzymology; D005399:Fishes / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D009132:Muscles / Q000201:enzymology; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism*; D012623:Seawater; D013045:Species Specificity; D013154:Spleen / Q000201:enzymology; D014018:Tissue Distribution
D010089:Oxidoreductases, N-Demethylating; C023186:trimethylamine dehydrogenase