Modification of glyceraldehyde-3-phosphate dehydrogenase, EC 1.2.1.12, isolated from rainbow trout during acclimation at 5 or 15 degrees C--II. Biochemical characterization of G3PDH muscle isolates.
G3PDH was isolated from the lateral muscle of rainbow trout (Salmo gairdneri) acclimated at 5 degrees C (cold) and 15 degrees C (warm). No differences were found in muscle concentration, molecular weights, isoelectric focusing patterns, amino acid compositions or peptide maps between cold and warm isolates. Cold and warm G3PDH contained mannose in variable concentration but no other prosthetic groups.
D000064:Acclimatization; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000302:isolation & purification* / Q000378:metabolism; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000302:isolation & purification; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D010446:Peptide Fragments / Q000032:analysis; D012487:Salmonidae / Q000502:physiology*; D013696:Temperature; D014337:Trout / Q000502:physiology*; D014357:Trypsin
D000596:Amino Acids; D007527:Isoenzymes; D010446:Peptide Fragments; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D014357:Trypsin