Humoral immune response of dogs after vaccination against leptospirosis measured by an IgM- and IgG-specific ELISA.
An IgM- and IgG-specific ELISA was used to measure the antibody response stimulated in dogs by vaccination with a leptospiral bacterin containing chemically inactivated Leptospira interrogans serotype icterohaemorrhagiae and serotype canicola leptospires. All dogs produced anti-leptospiral IgM and IgG. The IgM production was of the primary response type after each vaccination (primary vaccination, booster vaccination and annual revaccination). A substantial anti-leptospiral IgG response could be demonstrated only after the first booster vaccination and the annual revaccination. Annual revaccination resulted in a higher and much longer persisting IgG response than did the first booster vaccination. A revision of the vaccination scheme is suggested.
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D004283:Dog Diseases / Q000517:prevention & control; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007921:Leptospira interrogans / Q000276:immunology*; D007922:Leptospirosis / Q000517:prevention & control* / Q000662:veterinary; D008297:Male; D014611:Vaccination
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M