Inhibition of prostaglandin E synthesis by steroidal and nonsteroidal antiinflammatory drugs in human synovial fibroblast cultures.
Human synovial fibroblasts were "activated" by poly (I) X poly (C) to overproduce prostaglandin E (PGE). Hydrocortisone, diclofenac, indomethacin and ibuprofen inhibited PGE production in a dose dependent manner. Diclofenac (0.2 microgram/ml) had an effect similar to that of 1 microgram/ml of indomethacin and a stronger one than that of 0.5 microgram/ml hydrocortisone and 0.2 microgram/ml ibuprofen. Addition of hydrocortisone with either indomethacin or diclofenac had a synergistic inhibitory effect on PGE production. We suggest that this is an additional model for the study of PGE effect of antirheumatic drugs.
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004008:Diclofenac / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis; D006854:Hydrocortisone / Q000494:pharmacology; D007052:Ibuprofen / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D011070:Poly I-C / Q000037:antagonists & inhibitors; D011458:Prostaglandins E / Q000096:biosynthesis*; D013583:Synovial Membrane / Q000187:drug effects / Q000378:metabolism*
D000893:Anti-Inflammatory Agents; D011458:Prostaglandins E; D004008:Diclofenac; D006820:Hyaluronic Acid; D011070:Poly I-C; D006854:Hydrocortisone; D007052:Ibuprofen; D007213:Indomethacin