Catabolism of avian and mammalian haptoglobins and their complexes with haemoglobin in chicken.
The following 125I-labelled preparations of human and chicken haptoglobins (Hph, Hpc) and their complexes with human and chicken haemoglobins (Hph-Hbh, Hpc-Hbc, Hph-Hbc) were injected intravenously to chickens. Hpc exhibited long half-life time (t1/2 = 12.9 hr), whereas the homologous Hpc-Hbc complex disappeared from circulation faster (t1/2 = 3.1 hr). This suggested that the Hpc-Hbc complex was internalized by the receptor-mediated process in the avian liver. Mammalian Hph-Hbh complex was eliminated from chicken circulation rather fast (t1/2 = 0.5 hr) in contrast to "mixed" Hph-Hbc complex which was characterized by the relatively long half-life time (t1/2 = 4.4 hr). Hph-Hbc complex displayed 1-2 sites for binding an anti-Hph antibody i.e. less than Hph-Hbh or Hph-Hbc complexes. The Hph binding with Hbc seems to be weaker than with mammalian haemoglobins.
D000818:Animals; D002645:Chickens; D005260:Female; D006242:Haptoglobins / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D006736:Horses; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D046911:Macromolecular Substances; D013045:Species Specificity
D006242:Haptoglobins; D006454:Hemoglobins; D007457:Iodine Radioisotopes; D046911:Macromolecular Substances