Specificity and purification of antibodies to adenylic acid.
Anti-adenylate antibodies were elicited in rabbits using a conjugate of adenylic acid and bovine serum albumin as immunogen. The specificity of the antibodies was determined by monitoring the inhibition of the binding of [3H]AMP to the antibodies by various nonradioactive nucleic acid components, using a nitrocellulose filter assay. The antibodies were found to be directed against the whole molecule of AMP. They were reactive to polyadenylic acid, RNA, and denatured DNA. The hapten-specific antibodies were isolated by employing an AMP-AH-Sepharose affinity column.
D000249:Adenosine Monophosphate / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D008722:Methods; D011817:Rabbits; D012710:Serum Albumin, Bovine
D000906:Antibodies; D012710:Serum Albumin, Bovine; D000249:Adenosine Monophosphate