Different sensitivities of granulocyte-macrophage and erythroid progenitor cells of normal human bone marrow to S-phase-specific agents.
The extraordinary sensitivity of early erythroid progenitor cells (BFU-e) of normal human bone marrow to tritiated thymidine ([3H]TdR) was studied. While exposure of bone-marrow cells to [3H]TdR for 1 hr resulted in the death of only 40% of the granulocyte-macrophage progenitor cells (CFU-c), 90% of BFU-e were killed. Experiments in which normal bone-marrow cells were mixed with bone-marrow cells which had been exposed to [3H]TdR demonstrated that the excessive killing of BFU-e by [3H]TdR reflected carry-over of the [3H]TdR by the exposed cells. A carry-over effect was not observed for CFU-c, suggesting the presence of a fundamental difference in the metabolism of TdR between CFU-c and BFU-e. There was a suggestion of a carry-over effect regarding two other S-phase-specific agents, hydroxyurea and 1-beta-D-arabinofuranosylcytosine.
D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003561:Cytarabine / Q000633:toxicity*; D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects; D006098:Granulocytes / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D006918:Hydroxyurea / Q000633:toxicity*; D007399:Interphase / Q000187:drug effects* / Q000528:radiation effects; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects
D003561:Cytarabine; D006918:Hydroxyurea