Serological detection of H-Y antigen in humans with a cellular radioimmunobinding assay and monoclonal antibody.
A sensitive and reliable serological assay for the detection of H-Y antigen is described which uses monoclonal H-Y antibody and a cellular radioimmunobinding assay on cultured human fibroblasts. Cell lines from 2 normal human females, 2 normal human males and one XX human male were tested in 3 separate assays. Cells from XY and XX males were found to contain H-Y antigen; however, the reaction against the XX male cells was found to be intermediate between the XY male and normal XX female cells.
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D006182:H-Y Antigen / Q000032:analysis*; D006801:Humans; D008297:Male; D011863:Radioimmunoassay; D012729:Sex Chromosome Aberrations / Q000276:immunology
D000911:Antibodies, Monoclonal; D006182:H-Y Antigen