[The effect of x-rays on the level, composition and para-nitroanisole-o-demethylase activity of cytochrome P-450 in the liver microsomes of rats].
A study was made of the effect of X-radiation of different doses on the content of P-450 cytochrome in a microsomal fraction of rat liver. When the haemoprotein level markedly decreased an increase in Km and a decrease in Vmax were noted in the reaction of O-demethylation of para-nitroanisole by microsomes of the irradiated rat liver. It is suggested that one of the cause of the effect observed is the postirradiation change in the composition of cytochrome P-450 pool resulting from a selective decrease in the level of the radiosensitive forms of haemoprotein.
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism* / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology* / Q000528:radiation effects; D009575:Nitroanisole O-Demethylase / Q000378:metabolism* / Q000528:radiation effects; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D003577:Cytochrome P-450 Enzyme System; D009575:Nitroanisole O-Demethylase; D010088:Oxidoreductases
Vliianie rentgenovskogo oblucheniia na soderzhanie, sostav i para-nitroanizol-o-demetilaznuiu aktivnost' tsitokhroma P-450 v mikrosomakh pecheni krys.