Comparative study of avian ovalbumins by means of glycosidase treatment and following HPLC analysis of their dansyl glycopeptides.
Dansylated glycopeptides (DNS-GP) derived from ovalbumins (chicken, quail, goose and turkey) were treated with commercial endo-beta-N-acetyl-glucosaminidases (Endo-D and Endo-H). Susceptibility of DNS-GP components against those enzymes was examined by analysing the reaction product by HPLC. As reported previously (H. Iwase, Y. Kato and K. Hotta, 1984, Comp. Biochem. Physiol., 77B, 743-747), DNS-GP from chicken ovalbumin was separated into 13 peaks (C1-13) on HPLC. Beta-galactosidase treatment of the DNS-GP resulted in the disappearance of three peaks and the increase of their neighboring peaks. The results indicated that among 13 DNS-GP components from chicken ovalbumin, three (C6,8,11) were bearing galactose residue at the nonreducing terminal and another three (C7,9,12) would be bearing a galacto- sugar chain from each corresponded component.
D000818:Animals; D001717:Birds / Q000378:metabolism*; D002645:Chickens / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds; D004529:Egg White; D005260:Female; D005777:Geese / Q000378:metabolism; D006020:Glycopeptides / Q000032:analysis; D006026:Glycoside Hydrolases; D010047:Ovalbumin; D011784:Quail / Q000378:metabolism; D013045:Species Specificity; D014422:Turkeys / Q000378:metabolism
D003619:Dansyl Compounds; D006020:Glycopeptides; D010047:Ovalbumin; D006026:Glycoside Hydrolases