Nylon tube-immobilized creatinine iminohydrolase and glutamate dehydrogenase in serum and urine creatinine analysis.
Immobilized enzyme nylon-tube reactors incorporating creatinine iminohyrolase (CI) and glutamate dehydrogenase (GDH) were used to assay creatinine in serum and urine. Optimum substrate concentrations for the assay were determined. The reactors were incorporated into a continuous flow system for creatinine analysis. The method was evaluated with respect to linearity, sample interaction, precision, accuracy, and analytical recovery. Comparison studies were carried out with a standard Jaffé method and the effect of interfering substances was investigated. From the results obtained, it was concluded that the assay was suitable as a simple, reliable, and specific method for serum and urine creatinine determinations.
D000619:Aminohydrolases; D001322:Autoanalysis / Q000379:methods; D003404:Creatinine / Q000097:blood* / Q000652:urine; D004800:Enzymes, Immobilized; D005969:Glutamate Dehydrogenase; D006801:Humans; D007656:Ketoglutaric Acids / Q000032:analysis; D007700:Kinetics; D009243:NAD / Q000032:analysis; D009757:Nylons; D013056:Spectrophotometry, Ultraviolet
D004800:Enzymes, Immobilized; D007656:Ketoglutaric Acids; D009757:Nylons; D009243:NAD; D003404:Creatinine; D005969:Glutamate Dehydrogenase; D000619:Aminohydrolases; C023157:creatinine deiminase