title: Use of indigo carmine for twin amniocentesis and its effect on bilirubin analysis.
abstract: Indigo carmine is used commonly to mark the first-entered amniotic sac in twin amniocenteses. The presence of this dye significantly affects amniotic fluid bilirubin analysis. A method of chloroform extraction is recommended for determination of bilirubin in amniotic fluid previously contaminated by indigo carmine.
mesh_terms: D000649:Amniocentesis; D000653:Amniotic Fluid / Q000378:metabolism; D000743:Anemia, Hemolytic / Q000175:diagnosis; D001663:Bilirubin / Q000378:metabolism*; D004200:Diseases in Twins; D005189:False Positive Reactions; D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis; D006801:Humans; D007203:Indigo Carmine; D007211:Indoles; D011247:Pregnancy
chemical_list: D007211:Indoles; D007203:Indigo Carmine; D001663:Bilirubin