A new method for preparation of isolated single adult myocytes.
A new technique for isolation of single cardiac cells from adult rat ventricles is described. The mechanical method is modified from the method of Jacobson (Cell Struct. Funct. 2: 1-9, 1977) by employing an enzyme-free dispersion medium containing Sr2+ or Ba2+ to replace Ca2+. This method produces a high yield of isolated rod-shaped adult mammalian cardiomyocytes that do not contract spontaneously, have a normal resting potential, and are Ca2+ tolerant. This method represents a promising technique for isolation of single, viable, intact, adult myocytes.
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002469:Cell Separation / Q000379:methods*; D004361:Drug Tolerance; D004594:Electrophysiology; D006321:Heart / Q000187:drug effects / Q000502:physiology; D006352:Heart Ventricles / Q000166:cytology; D008297:Male; D009206:Myocardium / Q000166:cytology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013779:Tetrodotoxin / Q000494:pharmacology
D013779:Tetrodotoxin; D002118:Calcium