Participation of peroxisomal beta-oxidation system in the chain-shortening of a xenobiotic acyl compound.
A drug, (E)-3-[4-(1-imidazolylmethyl)phenyl]-2-propenoic acid, was metabolized to 4-(1-imidazolylmethyl)benzoic acid in isolated hepatocytes of rats, which was enhanced markedly by the pretreatment of rats with clofibrate. With liver homogenates, the formation of the CoA-ester of this drug and its subsequent chain-shortening were demonstrated. In the series of these reactions, acyl-CoA synthetase, CoA, ATP and NAD were required, whereas cyanide did not inhibit the reaction. These results indicate that peroxisomes are capable of shortening the acyl side-chains of drugs by the beta-oxidation, giving an additional suggestion on the functions of peroxisomes.
D000179:Acrylates / Q000378:metabolism*; D000284:Administration, Oral; D000818:Animals; D002994:Clofibrate / Q000494:pharmacology; D003486:Cyanides / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000648:ultrastructure*; D008297:Male; D008689:Methacrylates / Q000378:metabolism*; D008830:Microbodies / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000179:Acrylates; D003486:Cyanides; D008689:Methacrylates; D002994:Clofibrate; C034364:ozagrel