title: Production and characterization of antibody against diacetoxyscirpenol.
abstract: Antibodies against diacetoxyscirpenol (DAS) were obtained from rabbits after immunizing them with hemisuccinate or hemiglutarate derivatives of DAS conjugated to bovine serum albumin (BSA). DAS-hemiglutarate-BSA was found to be a much better immunogen than DAS-hemisuccinate-BSA. Competitive radioimmunoassay revealed that the antisera obtained from rabbits after immunization with DAS-hemiglutarate-BSA showed high specificity toward DAS. The concentrations causing 50% displacement of radioactive DAS by unlabeled DAS, 4-monoacetoxyscirpenol (MAS), and 15-MAS were found to be 1.5, 130, and 300 ng per assay, respectively. Thus, the cross-reactivities for 4-MAS and 15-MAS are ca. 87 and 300 times weaker than that of DAS. Practically no cross-reaction (less than 5% displacement) was observed when deoxynivalenol, T-2 toxin, deoxyverrucarol, and scirpentriol were tested at a concentration of 2,000 ng/ml.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000972:Antineoplastic Agents, Phytogenic / Q000276:immunology*; D003429:Cross Reactions; D007114:Immunization; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology; D012717:Sesquiterpenes / Q000276:immunology*; D013605:T-2 Toxin / Q000276:immunology; D014255:Trichothecenes / Q000276:immunology*
chemical_list: D000906:Antibodies; D000972:Antineoplastic Agents, Phytogenic; D012717:Sesquiterpenes; D014255:Trichothecenes; D012710:Serum Albumin, Bovine; D013605:T-2 Toxin; C001809:diacetoxyscirpenol