Correlations between iron content and isoferritin profiles of normal and malignant tissues.
Isoelectric focusing profiles of ferritins isolated from livers of anemic rats and 3'-methyl-4-dimethylaminoazobenzene-induced hepatomas were more acidic than their normal counterparts, and exhibited patterns similar to those of heart ferritin. Iron administration induced a shift to more basic components for the normal and tumor ferritins. The isoferritin patterns reflected the subunit populations of the protein, as the more acidic components had greater proportions of H-type subunits and iron induced an increase in L subunits with concomitant increases in more basic isoferritins. For both normal and malignant tissues there was a direct correlation between iron content of the tissue and subunit composition. The apparently unique isoferritin patterns in the tumor tissue may, therefore, be ascribed primarily to the lower iron contents.
D000740:Anemia / Q000378:metabolism; D000818:Animals; D005293:Ferritins / Q000032:analysis*; D007501:Iron / Q000032:analysis* / Q000494:pharmacology; D008099:Liver / Q000032:analysis; D008297:Male; D009206:Myocardium / Q000032:analysis; D009374:Neoplasms, Experimental / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D005293:Ferritins; D007501:Iron