Cell culture mutants as aminoacyl-tRNA synthetase complex probes.
A number of Chinese hamster ovary cell culture mutants have been characterized by us for their high-molecular-weight aminoacyl-tRNA synthetase (aaRS) complexes. The results all support a model of aaRS complex function wherein the high-molecular-weight complexes form aminoacyl-tRNA by utilizing extracellular amino acids immediately on their transport and before they have equilibrated with the internal pool. The low-molecular-weight aaRS forms exclusively utilize amino acids from only the intracellular pool.
D000596:Amino Acids / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D000818:Animals; D002451:Cell Compartmentation; D002478:Cells, Cultured / Q000201:enzymology*; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D008954:Models, Biological; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000235:genetics*; D009154:Mutation; D010053:Ovary
D000596:Amino Acids; D009097:Multienzyme Complexes; D000604:Amino Acyl-tRNA Synthetases