Influence of Concanavalin A on 3-O-methylglucose uptake in cultured chick embryo fibroblasts. Evidence for differences related to the age of embryos.
Concanavalin A (Con A) was found to inhibit hexose uptake in cultured fibroblasts derived from 8-day chick embryos and to stimulate this process in those derived from 16-day embryos. Con-A effects depended on the duration of contact with cells and lectin and were inhibited by alpha-methylmannopyrannoside. Con A was shown to mask about 70% of the hexose carriers in both 8- and 16-day embryo fibroblasts. Lectin altered the hexose uptake very rapidly. Con A only modified the Vmax of the uptake system and did not alter the Km. This indicates that either the number or mobility of hexose carriers were modified by Con-A treatment. The differential effect of lectin could be due to a modification of the hexose-carrier mobility during the embryonic differentiation of fibroblasts. Secondary effects may affect cell growth.
D019325:3-O-Methylglucose; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002642:Chick Embryo; D003208:Concanavalin A / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D013997:Time Factors
D008757:Methylglucosides; D008759:Methylglycosides; D003208:Concanavalin A; D019325:3-O-Methylglucose