Chemiluminescent assay of lipid peroxide in plasma using cytochrome c heme peptide.
A convenient assay specific to lipid hydroperoxide in plasma is presented. Cytochrome c heme peptide obtained from Saccharomyces was found to emit a strong chemiluminescence with any hydroperoxide, but not with TBA-reactive substances. The benefit of measuring this luminescence using photon counting is discussed with respect to in vivo lipid peroxidation.
D000818:Animals; D001095:Arachidonic Acids / Q000097:blood; D006420:Hemeproteins / Q000378:metabolism*; D015289:Leukotrienes; D008054:Lipid Peroxides / Q000097:blood*; D049449:Luminescence; D051379:Mice; D008807:Mice, Inbred BALB C; D010544:Peroxidases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012440:Saccharomyces
D001095:Arachidonic Acids; D006420:Hemeproteins; D015289:Leukotrienes; D008054:Lipid Peroxides; C025086:15-hydroperoxy-5,8,11,13-eicosatetraenoic acid; D010544:Peroxidases; C021799:microperoxidase