Ultrastructural localization of secretin in endocrine cells of the dog duodenum by the immunogold technique. Comparison with ultrastructurally characterized S cells of various mammals.
Secretin has been localized by the immunogold technique in endocrine cells of the dog duodenum--previously described as "K cells"--characterized by secretory granules with double structure consisting of a secretin-containing osmiophilic core surrounded by an argyrophil halo. Granules resembling those of dog secretin cells were also found in some ultrastructurally characterized S cells of the cat, pig, rat and rabbit duodenum, thus confirming in these species the identification of S cells with secretin cells. Conversely, the cells previously described as "S cells" in the dog lacked secretin immunoreactivity.
D000818:Animals; D002415:Cats; D002838:Chromaffin System / Q000502:physiology; D004285:Dogs; D004386:Duodenum / Q000032:analysis* / Q000648:ultrastructure; D004702:Endocrine Glands / Q000032:analysis* / Q000648:ultrastructure; D006046:Gold; D006651:Histocytochemistry; D007120:Immunochemistry / Q000379:methods; D007413:Intestinal Mucosa / Q000166:cytology; D011817:Rabbits; D051381:Rats; D012633:Secretin / Q000032:analysis*; D013045:Species Specificity; D013205:Staphylococcal Protein A / Q000276:immunology; D013552:Swine
D013205:Staphylococcal Protein A; D012633:Secretin; D006046:Gold