Comparison of mice and cell cultures for the isolation of tick-borne viruses.
Three methods of isolating viruses from 10 tick pools were compared; none of the methods produced all 13 of the viruses isolated (7 viruses of the bunyaviridae and 6 orbiviruses). Inoculation of homogenised ticks into various cell lines was the most successful, yielding 11 virus isolations. Only 2 tick homogenates induced overt signs of infection following intra-cerebral inoculation of 2-day-old mice. However, when inoculated mouse brain was passaged in various cell lines, 8 of 12 isolations were made. The rates of success of the 3 methods of virus isolation appeared to vary according to the type and titre of the virus in the tick pool.
D000818:Animals; D001717:Birds / Q000469:parasitology; D001921:Brain / Q000382:microbiology; D002043:Bunyaviridae / Q000302:isolation & purification*; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D006224:Cricetinae; D005260:Female; D007668:Kidney; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D012087:Reoviridae / Q000302:isolation & purification*; D013987:Ticks / Q000382:microbiology*; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication; D014982:Xenopus laevis