title: Isolation of toxigenic strains of Pasteurella multocida from lungs of pneumonic swine.
abstract: Lungs from 113 pneumonic pigs were examined for Pasteurella multocida. The lungs were smeared directly onto blood agar and homogenized in brain-heart infusion broth and then inoculated intraperitoneally in mice. Pasteurella multocida isolates were typed for serotypes A (by hyaluronidase inhibition of capsule) and D (by acriflavine autoagglutination). Strains were tested for toxin production by intradermal injection of 0.2 ml of filtered 24-hour culture supernatants into guinea pigs. Most lungs (70.8%) yielded isolations. Most isolants (87.5%) were type A and 12.5% were type D. Of the type D strains, 80% were toxigenic. Of the type A isolants, 18.2% were toxigenic.
mesh_terms: D000818:Animals; D008168:Lung / Q000382:microbiology*; D010325:Pasteurella / Q000302:isolation & purification; D010326:Pasteurella Infections / Q000662:veterinary*; D011014:Pneumonia / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*