Sorption of organic compounds from urine in mutagenicity testing: choice of sorbent.
The choice of sorbents for urine mutagenicity testing was investigated. 14C-Labelled compounds were dissolved in urine and extracted from it by sorption with seven different sorbents singly or in combination. The compounds were desorbed with acetone. Sorption was examined under various experimental conditions. Except for two strong ionic compounds satisfactory extraction was achieved with a combination of three sorbents: Amberlite XAD-2, XAD-7, and Sep-Pak Silica. Filtration of the urine or contact of it with the walls of the experimental equipment could lead to substantial losses. Implications of these results for urine mutagenicity testing and possibilities for further optimization of the method are discussed.
D000327:Adsorption; D002852:Chromatography, Ion Exchange / Q000379:methods; D005898:Glass; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D009153:Mutagens / Q000652:urine*; D012997:Solvents; D014462:Ultrafiltration
D008567:Membranes, Artificial; D009153:Mutagens; D012997:Solvents