title: Separation, isolation and amino acid composition of uremic peptides.
abstract: Gel filtration and thin layer chromatography were conducted on sera from uremic patients and normal subjects for the isolation of nitrogenous substances unique to uremia. Many ninhydrin-positive substances were found in greater amounts in uremic patients compared to normal subjects. Some of these ninhydrin-positive substances were also detected by staining with chlorine-tolidine. Amino acid analysis of these substances showed considerable qualitative and quantitative differences, perhaps reflecting interference with enzymatic activity by the uremic environment.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D006801:Humans; D010455:Peptides / Q000097:blood* / Q000302:isolation & purification; D014118:Toxins, Biological / Q000097:blood* / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; D010455:Peptides; D014118:Toxins, Biological; C026865:uremia middle molecule toxins