A simplified procedure for the preparation of tritiated GM1 ganglioside and other glycosphingolipids.
The ganglioside II3NeuAc-GgOse4Cer and other glycosphingolipids can be radiolabeled to high specific activity by the galactose oxidase-NaB3H4 procedure, by purifying the oxidized compounds prior to reductive labeling. The oxidized products are separated from nonoxidized compounds and detergents (Triton X-100 and sodium taurocholate) present during the enzymatic oxidation. Since the oxidized derivatives are separated, the final specific activity depends solely upon the specific activity of the NaB3H4 and the reduction conditions.
D000818:Animals; D001894:Borohydrides; D002417:Cattle; D002850:Chromatography, Gel; D005677:G(M1) Ganglioside / Q000302:isolation & purification*; D005692:Galactose Oxidase; D005732:Gangliosides / Q000302:isolation & purification*; D006028:Glycosphingolipids / Q000302:isolation & purification*; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D010084:Oxidation-Reduction; D014316:Tritium
D001894:Borohydrides; D005732:Gangliosides; D006028:Glycosphingolipids; D014316:Tritium; D005677:G(M1) Ganglioside; C025364:sodium borohydride; D005692:Galactose Oxidase