Ion-exchange, gel-filtration and reversed-phase high-performance liquid chromatography in the isolation of neurotensin-degrading enzymes from rat brain.
Brain and pituitary peptidases involved in the metabolism of neurotensin were identified by ion-exchange (TSK-545 DEAE) and gel-filtration (TSK-G4000 SW) high-performance liquid chromatography (HPLC). Reversed-phase HPLC on radial compression cartridges was used in a rapid assay of neurotensin-hydrolysing activity in column fractions and at the same time the bond specificity of the activity was determined. Semipreparative isolation of the major enzyme which cleaves neurotensin at Arg8-Arg9 was carried out and some of its characteristics were determined.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D006868:Hydrolysis; D009496:Neurotensin / Q000378:metabolism*; D010902:Pituitary Gland / Q000201:enzymology; D051381:Rats
D000596:Amino Acids; D009496:Neurotensin