A gene regulating the time dependence of alpha-L-fucosidase concentration is closely linked with the structural gene in man.
The dependence of the specific activities of human alpha-L-fucosidase and four other lysosomal glycosidases on cell growth was determined in long-term lymphoid cell lines in which the polymorphic forms of alpha-L-fucosidase were identified by isoelectric focusing. In cultured lymphoblasts of Fu-1 phenotype the alpha-L-fucosidase synthesis during cell growth was found to be proportional to the general amount of protein. However, in cells derived from individuals possessing at least one FUCA*2 allele, the amount of enzyme increased progressively during the log-phase of growth, reaching a maximum at the transition to the stationary phase. The maximum increase of enzyme concentration was observed in the mid-log phase of exponential growth. The results may be explained by the existence of a regulatory locus FUCT which maps in close proximity to the structural gene. The FUCT locus which controls the amount of enzyme in the cell appears to be specific for alpha-L-fucosidase.
D002455:Cell Division; D002460:Cell Line; D002874:Chromosome Mapping; D002900:Chromosomes, Human, 1-3 / Q000648:ultrastructure*; D005796:Genes; D005809:Genes, Regulator; D008040:Genetic Linkage; D006801:Humans; D007525:Isoelectric Focusing; D008214:Lymphocytes / Q000166:cytology / Q000648:ultrastructure; D010641:Phenotype; D011110:Polymorphism, Genetic; D013997:Time Factors; D005644:alpha-L-Fucosidase / Q000235:genetics*
D005644:alpha-L-Fucosidase