[Hydroxylation products of hydrophobic xenobiotics--stabilizers of cytochrome P-450 in the hepatocytes].
The effects of the hydroxylation product 3,4-benzo(a)pyrene and the free radical scavenger 1,2,3-trioxybenzene on cytochrome P-450 degradation in isolated rat hepatocytes induced by the Fe2+-ADP + NADPH system activating lipid peroxidation (LPO) were investigated. During incubation of hepatocytes, cytochrome P-450 is destroyed due to accumulation of LPO products. Addition of the free radical scavenger 1,2,3-trioxybenzene and the monoxygenase substrate 3,4-benzo(a)pyrene to the incubation medium induces inhibition of LPO and simultaneous stabilization of cytochrome P-450. Deceleration of malonic dialdehyde production by the free radical scavenger of the monoxygenase substrate suggests that both the compounds stabilize cytochrome P-450. It is assumed that in liver hepatocytes, exogenous free radical scavengers of the phenolic type and the products of their decarboxylation protect cytochrome P-450 against the LPO-induced destruction via oxidative metabolism of hydrophobic substrates.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001564:Benzo(a)pyrene / Q000378:metabolism / Q000494:pharmacology*; D001711:Biotransformation / Q000187:drug effects; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004790:Enzyme Induction / Q000187:drug effects; D005296:Ferrous Compounds / Q000494:pharmacology; D006900:Hydroxylation; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008315:Malondialdehyde / Q000378:metabolism; D009249:NADP / Q000494:pharmacology; D011748:Pyrogallol / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D013997:Time Factors
D000975:Antioxidants; D005296:Ferrous Compounds; D008054:Lipid Peroxides; D011748:Pyrogallol; D001564:Benzo(a)pyrene; D008315:Malondialdehyde; D009249:NADP; D000244:Adenosine Diphosphate; D003577:Cytochrome P-450 Enzyme System
Produkty gidroksilirovaniia gidrofobnykh ksenobiotikov--stabilizatory tsitokhroma P-450 v gepatotsitakh.