title: Pulmonary macrophage: a major source of lipoprotein lipase in the lung.
abstract: The lipase released by heparin infusion into perfused rat lungs is shown to be identical with lipoprotein lipase on the basis of the following characteristics: 1) it required plasma for full activity; 2) it was largely inhibited by 1 M NaCl; and 3) it bound to a heparin-Sepharose gel and eluted with buffer containing 1.5 M NaCl. Macrophages prepared from rat lungs released a considerable amount of lipoprotein lipase, with characteristics similar to those of the lipoprotein lipase released from heparin-perfused rat lungs. Pulmonary surfactant-producing type II pneumocytes did not cause a significant release of lipoprotein lipase. No apparent lipoprotein lipase activities were detected in the conditioned medium of other lung cells from which macrophages had been selectively removed.
mesh_terms: D000818:Animals; D001769:Blood; D002846:Chromatography, Affinity; D006493:Heparin / Q000378:metabolism; D008049:Lipase / Q000032:analysis; D008071:Lipoprotein Lipase / Q000032:analysis*; D008168:Lung / Q000166:cytology*; D008264:Macrophages / Q000201:enzymology*; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D006493:Heparin; D008049:Lipase; D008071:Lipoprotein Lipase