title: Fibronectin mediated human gingival fibroblasts attachment to bone.
abstract: Experiments were undertaken to examine the effects of acid and fibronectin treatments on the human gingival fibroblast attachment to bone in vitro. The binding of [125I]fibronectin to bone powders was dependent on concentration of fibronectin and incubation time. The optimum pH of [125I]fibronectin attachment to bone powders was 6.0 using phosphate buffer. Acid treatment on bone powders in low concentration such as 10 mM of citric acid enhanced [125I]fibronectin binding. The attachment of [3H]thymidine labelled human gingival fibroblasts to bone was enhanced 1.5, 2.0 and 2.5-fold by citric acid, fibronectin and citric acid + fibronectin treatment, respectively. Scanning electron microscopic observations confirm that citric acid and/or fibronectin treatment enhanced the cell number and rapidity of fibroblast attachment to bone surfaces.
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D005353:Fibronectins / Q000378:metabolism* / Q000494:pharmacology; D005881:Gingiva / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011485:Protein Binding / Q000187:drug effects; D013552:Swine
chemical_list: D002951:Citrates; D005353:Fibronectins; D019343:Citric Acid