Inhibition of polymorphonuclear leukocyte functions by fluorinated nitrobenzenes.
The bifunctional fluorinated nitrobenzenes, 1,5-difluoro-2,4-dinitrobenzene (DFDNB) and 4,4'-difluoro-3,3'-dinitrodiphenyl sulfone (DFDNDPS), and the monofunctional 1-fluoro-2,4-dinitrobenzene (FDNB) inhibit chemotaxis, phagocytosis, exocytosis and the respiratory burst of rabbit polymorphonuclear leukocytes. Inhibition occurs in the micromolar concentration range; the bifunctional compounds are stronger inhibitory than the monofunctional one. The inhibitory effect can be counteracted by sulfhydryl compounds and not with amino-group containing compounds. The results suggest that an interaction with vulnerable sulfhydryl groups, located in a hydrophobic surrounding, is the basis of the inhibitory effect of the fluorinated nitrobenzenes.
D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology; D004139:Dinitrofluorobenzene / Q000031:analogs & derivatives / Q000494:pharmacology*; D005089:Exocytosis / Q000187:drug effects; D007700:Kinetics; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D009578:Nitrobenzenes / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects; D011817:Rabbits; D013329:Structure-Activity Relationship
D003432:Cross-Linking Reagents; D009578:Nitrobenzenes; C013936:difluorodinitrobenzene sulfone; D003571:Cytochalasin B; D009240:N-Formylmethionine Leucyl-Phenylalanine; D004139:Dinitrofluorobenzene; C002041:1,5-difluoro-2,4-dinitrobenzene