Purification of S-2-hydroxyacylglutathione hydrolase (Glyoxalase II) from calf brain.
S-2-Hydroxyacylglutathione hydrolase (Glyoxalase II) from calf brain has been purified 8333-times compared to 65,000 g supernatant of brain homogenate. The purification procedure employs Affi-Gel blue and preparative isoelectric focussing and offers a suitable method for the preparation of highly purified enzyme. Calf brain Glyoxalase II is a basic protein with a pl of 7.63 determined by isoelectric focusing. An evaluation of the relative molecular mass by gel filtration gave a value of about 23,000. During the purification procedure a constant Km value of about 0.325 mM was observed. A turnover number of 16,100 min-1 was calculated for the purified enzyme.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002846:Chromatography, Affinity; D007525:Isoelectric Focusing; D007700:Kinetics; D008970:Molecular Weight; D013869:Thiolester Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D000099301:Hydroxyacylglutathione Hydrolase
D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase