Some characteristics of urea accumulation in the renal cortex tissue of rats and dogs.
The urea concentration in the renal cortex ([RC]), liver ([L]) and skeletal muscle ([SM]) of non-diuretic Wistar rats was measured chemically and after an i.m. injection of 14C-urea and was compared with the plasma urea concentration ([P]). [L]/[P] and [SM]/[P] always equalled 1, irrespective of whether they were measured chemically or by means of radioactivity. [RC]/[P] was 2.81, again without any difference between chemical and radioactive measurement. The ratio of the chemically measured urea concentration in the renal cortex and plasma of mongrel dogs was 5.71, i.e. significantly higher than in rats (p less than 0.01). The intrarenal infusion of KCN, iodoacetate and ouabain did not alter it significantly (5.52, p greater than 0.05). Active transport, in whatever form, does not seem to be the cause of the high urea concentration in rat and dog renal cortex.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001693:Biological Transport, Active / Q000187:drug effects; D002250:Carbon Radioisotopes; D004285:Dogs; D005260:Female; D007461:Iodoacetates / Q000494:pharmacology; D019807:Iodoacetic Acid; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D011190:Potassium Cyanide / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D014508:Urea / Q000378:metabolism*
D002250:Carbon Radioisotopes; D007461:Iodoacetates; D010042:Ouabain; D014508:Urea; D011190:Potassium Cyanide; D019807:Iodoacetic Acid