Lithocholate detoxification and biliary secretion in the rat.
To define the efficiency of hepatic detoxification, 14C lithocholate in combination with taurocholate was continuously infused intravenously into rats until steady-state. Quantitation of hepatic radiolabelled bile acid under these conditions showed only 11% of total liver bile acid was unmetabolised, indicating very efficient detoxification of lithocholate, in its most hepatotoxic state. Interestingly we found the rate for each bile acid to reach steady-state differed. To test whether the difference was due to glutathione S-transferase binding, as proposed by Strange et al (8), glutathione S-transferase levels were induced by phenobarbitone treatment. An increase in cytosol glutathione S-transferase levels had no effect on the time it took for each bile acid to reach steady-state.
D000818:Animals; D001646:Bile / Q000378:metabolism*; D002384:Catalysis; D003600:Cytosol / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008658:Inactivation, Metabolic; D008095:Lithocholic Acid / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013656:Taurocholic Acid / Q000378:metabolism
D013656:Taurocholic Acid; D008095:Lithocholic Acid; D005982:Glutathione Transferase; D010634:Phenobarbital