title: Isolation of two different pools of pituitary prolactin.
abstract: Two pools of prolactin (Prl) were isolated from the pituitary gland. One readily soluble after homogenization and the other sedimented with secretory granules. In ovariectomized-oestrogen treated rats the latter represents the major Prl fraction of pituitary and can be recovered as immunoreactive Prl after extraction in 2.5 M urea. By correlation of electron microscopy, chromatography and RIA, evidence is gathered that the soluble fraction is constituted by monomeric (little) Prl and that secretory granules contain Prl in a polymerized form (big).
mesh_terms: D000818:Animals; D002369:Castration; D002850:Chromatography, Gel; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D008854:Microscopy, Electron; D010903:Pituitary Gland, Anterior / Q000032:analysis* / Q000648:ultrastructure; D011388:Prolactin / Q000302:isolation & purification*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004958:Estradiol; D011388:Prolactin