title: Characterization and function of isolated hemoglobins from the marine teleost Girella tricuspidata.
abstract: Five isohemoglobins were characterized from the marine teleost fish, Girella tricuspidata, using polyacrylamide gel electrophoresis and isoelectric focusing. O2-equilibrium (OEC) studies of the five components separated by column chromatography showed functional heterogeneity with respect to Hill's coefficient n, the O2 affinity, P50, and the Bohr coefficient phi, = delta logp50/delta pH. Recombination and examination of the isolates gave an OEC similar to that of "stripped" hemolysate and similar to a curve synthesized from a model incorporating the OEC parameters of the isoHbs.
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes / Q000097:blood*; D006454:Hemoglobins / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D010108:Oxyhemoglobins / Q000378:metabolism
chemical_list: D006454:Hemoglobins; D010108:Oxyhemoglobins