title: Purification of Giardia muris cysts by velocity sedimentation.
abstract: Giardia muris cysts were separated from fecal contaminants in primary isolates by unit gravity velocity sedimentation. Crude isolates obtained by centrifugation over 1.0 M sucrose were overlaid onto a Percoll density gradient, 1.01 to 1.03 g/ml. G. muris cysts were well separated from faster-sedimenting fecal debris and slower-sedimenting Spironucleus muris and bacteria in 1.5 h.
mesh_terms: D002499:Centrifugation, Density Gradient / Q000295:instrumentation / Q000379:methods; D005243:Feces; D005872:Giardia / Q000302:isolation & purification*; D006801:Humans