Direct spectrophotometric analysis of hemoglobin in isoelectric focusing tube gels.
Methods are described for the direct spectrophotometric analysis of human oxyhemoglobin, deoxyhemoglobin, and methemoglobin focused in polyacrylamide tube gels. Visible absorption spectra (350-650 nm) of electrofocused hemoglobin bands were recorded using a diode array rapid-scan spectrophotometer. Direct optical sampling of gels allowed the identification of focused hemoglobin valency hybrids which contain two oxidized monomers per tetramer.
D005782:Gels; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D007525:Isoelectric Focusing; D008706:Methemoglobin / Q000032:analysis; D010108:Oxyhemoglobins / Q000032:analysis; D013053:Spectrophotometry
D005782:Gels; D006454:Hemoglobins; D010108:Oxyhemoglobins; C100003:deoxyhemoglobin; D008706:Methemoglobin