Localization, isolation and characterization of Mn-superoxide dismutase in bovine adrenocortical cells.
Cyanide-insensitive superoxide dismutase activity was present in both cytosol (26%) and mitochondrial (64%) fractions of bovine adrenal cells. The cyanide-insensitive superoxide dismutase was isolated from the mitochondrial fraction. It contained 2.2 g atoms of manganese per mol of enzyme. The enzyme had a molecular weight of 82,000 and a subunit molecular weight of 22,000. The isoelectric point, amino acid composition, and spectra of visible and ultraviolet regions were similar to those of the Mn-superoxide dismutase from the other mitochondria.
D000302:Adrenal Cortex / Q000201:enzymology* / Q000648:ultrastructure; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D003300:Copper / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D007526:Isoelectric Point; D007527:Isoenzymes / Q000302:isolation & purification; D046911:Macromolecular Substances; D008345:Manganese / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology; D008970:Molecular Weight; D013482:Superoxide Dismutase / Q000302:isolation & purification*; D014018:Tissue Distribution; D015032:Zinc / Q000378:metabolism
D000596:Amino Acids; D007527:Isoenzymes; D046911:Macromolecular Substances; D008345:Manganese; D003300:Copper; D013482:Superoxide Dismutase; D015032:Zinc