Simultaneous determination of thiamine and riboflavin in foods by liquid chromatography.
Recent methods for determination of thiamine (thiochrome) and riboflavin by liquid chromatography (LC) are outlined and discussed, and a new method allowing the simultaneous determination of these 2 vitamins by using a single fluorescence detector is described. This system involves an ODS 5 micron ultrasphere column and a pH 7.5 mobile phase composed of 0.005M tetrabutyl ammonium phosphate in methanol-water (20 + 80).
D002853:Chromatography, Liquid / Q000379:methods; D005504:Food Analysis; D012256:Riboflavin / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013831:Thiamine / Q000032:analysis*
D012256:Riboflavin; D013831:Thiamine