[The effect of exogenous tRNA from different sources on the biosynthesis of milk proteins].
The tRNA dependent cell--free protein--synthesizing system from rabbit differentiated mammary gland has been obtained. The level of protein synthesis including caseins was found to be much higher in the presence of homologous tRNA in comparison with tRNAs from non-differentiated mammary gland, liver, brewer's yeast. The efficiency of translation was shown to depend on the tRNA pool quantitative balance. The addition of tRNA to mammary gland explants causes stimulation of casein synthesis. The level of this stimulation depends on both the origin of tRNA and physiological state of the gland. It is concluded that the functional adaptation of tRNA is a regulatory link in specific protein biosynthesis at the translation level.
D000818:Animals; D002364:Caseins / Q000096:biosynthesis; D002474:Cell-Free System; D046508:Culture Techniques; D005260:Female; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008894:Milk Proteins / Q000096:biosynthesis*; D011247:Pregnancy; D012331:RNA, Fungal / Q000494:pharmacology; D012343:RNA, Transfer / Q000494:pharmacology*; D011817:Rabbits
D002364:Caseins; D008894:Milk Proteins; D012331:RNA, Fungal; D012343:RNA, Transfer
Vliianie ékzogennykh tRNK razlichnogo proiskhozhdeniia na biosintez belkov moloka.