[Intracellular and extracellular ribonucleases of Bacillus intermedius].
Endocellular and exocellular ribonucleases were studied in Bacillus intermedius. Two fractions of ribonucleases (Rf 0.72 and 0.96) were found to be associated with the cellular surface and seven fractions (Rf 0.1, 0.17, 0.33, 0.45, 0.72, 0.82 and 0.96) were detected in the cytoplasm. RNAase with Rf 0,096 had the highest activity and was repressed by inorganic orthophosphate. This RNase accumulated in the cell during the stationary growth phase just as the free enzyme form did in the culture medium. The immunological characteristics of these enzymes were identical as was shown by immunochemical analysis.
D001407:Bacillus / Q000201:enzymology*; D002473:Cell Wall / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D005095:Exoribonucleases / Q000032:analysis / Q000096:biosynthesis*; D007122:Immunoelectrophoresis; D012260:Ribonucleases / Q000032:analysis / Q000096:biosynthesis*
D005095:Exoribonucleases; D012260:Ribonucleases
Izuchenie vnutrikletochnykh i vnekletochnykh ribonukleaz Bacillus intermedius.