Taurine transport in brush border membrane vesicles isolated from hyper- and normotaurinuric mouse kidney.
The mouse strain C57BL is characterized by a high urinary fractional excretion of taurine. Hypertaurinuric [tau(-)] C57BL mice and three normal taurine 'excretors' [tau(+)]-Swiss, A/J, and C3H-were compared. Clearance experiments showed that fractional excretion of taurine was 4-8 times higher in C57BL relative to the other strains. Transport studies performed in brush border membrane vesicles, isolated from C57BL and C3H mouse renal cortex, showed that D-glucose uptake was similar in both strains while the taurine uptake appeared to be faster in tau(+) than in tau(-) mice. This difference was observed only in the presence of an inwardly directed NaCl gradient. The comparison of kinetic parameters showed a significant difference of apparent Km values: 90.8 +/- 11.4 microM in tau(+) mice vs. 129.5 +/- 17.1 microM in tau(-) mice. Jmax were not significantly different in both strains. These observations suggest a lower affinity of the taurine brush border membrane carrier in the hypertaurinuric strain as compared to the normotaurinuric mice. It is concluded that, in addition to a possible alteration of peritubular taurine efflux described by Rozen et al. (1983), a defect of taurine transport at the luminal membrane cannot be excluded and could account for the hypertaurinuria of the C57BL mice.
D000818:Animals; D001692:Biological Transport; D005260:Female; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D008871:Microvilli / Q000378:metabolism*; D013654:Taurine / Q000378:metabolism* / Q000652:urine
D013654:Taurine