Chromosomal transplantation. The nuclear transplantation of colchicine-treated cells.
Dissociated cells of middle-to-late blastulae were exposed to 0.1 mg colchicine/ml and achieved 92% metaphase arrest. These cells contained a haploid set of Bombina maxima (Anura:Discoglossidae) chromosomes. When transplanted into the enucleated eggs of B. orientalis, some donor cells stimulated development to the late blastula and middle gastrula stages. - Most (17/20) of the embryos resulting from chromosomal transplantation were nonmosaic aneuploids. A high percentage of recipient egg enucleation (93%), the ratio of long-to-short chromosomes, and the presence of species-specific marker chromosomes proved that chromosomes were transplanted from the donor cells. Therefore, metaphase chromosomes lacking intact spindle apparatuses were injected into and incorporated by amphibian eggs. These chromosomes were replicated in all cells of the resulting embryos. The aneuploidy of these embryos is explained by an inability of the recipient egg to locate and replicate many transplanted chromosomes (44%) before first cleavage.
D000782:Aneuploidy; D000818:Animals; D001001:Anura; D001755:Blastocyst / Q000187:drug effects / Q000502:physiology; D002875:Chromosomes / Q000187:drug effects / Q000637:transplantation*; D003078:Colchicine / Q000494:pharmacology*; D003433:Crosses, Genetic; D004625:Embryo, Nonmammalian / Q000502:physiology; D005260:Female; D007621:Karyotyping; D008297:Male; D008940:Mitotic Index; D053652:Nuclear Transfer Techniques
D003078:Colchicine