Detection of tumor-inducing plasmid DNA sequence in Agrobacterium tumefaciens by DNA-DNA hybridization.
Absence of plasmid DNA sequences in non-tumorigenic (crown gall tumor) strains of Agrobacterium tumefaciens was confirmed by DNA-DNA hybridization between purified tumor-inducing (Ti) plasmid DNAs isolated from the progenitor tumorigenic strains and 3H-labeled whole cell DNAs from tumorigenic strains and their non-tumorigenic derivatives. The genes controlling tumorigenicity in plant and utilizability of nopaline as their sole nitrogen source were confirmed to locate on Ti-plasmids.
D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial; D009693:Nucleic Acid Hybridization; D010941:Plant Tumors / Q000382:microbiology*; D010957:Plasmids; D012231:Rhizobium / Q000235:genetics* / Q000378:metabolism
D004269:DNA, Bacterial; C008666:nopaline; D001120:Arginine