Simultaneous detection of native and luminol-dependent luminescence of stimulated human polymorphonuclear leukocytes.
A method for investigating the cellular response of polymorphonuclear leukocytes to various stimuli was introduced using simultaneously native (luminol-independent) and luminol dependent luminescence as an indicator for myeloperoxidase (MPO)-H2O2-halide and O2- mediated reactions. In experimental systems containing low concentrations of luminol the total light emission was separated into contributions of native and luminol-dependent luminescence by making use of the different spectral behaviour of the two kinds of luminescence. Consequently the MPO-H2O2-halide system could be distinguished from the O2- dependent system by interpreting the recorded temporal traces of the emitted light.
D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003571:Cytochalasin B / Q000494:pharmacology; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D009842:Oligopeptides / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects; D011724:Pyridazines / Q000494:pharmacology*; D015054:Zymosan / Q000494:pharmacology
D009842:Oligopeptides; D011724:Pyridazines; D003571:Cytochalasin B; D008165:Luminol; C024998:N-formylnorleucyl-leucyl-4-phenylalanine; D015054:Zymosan