Increased synthesis of mouse minor hemoglobin in erythroid colonies: a cellular model for hemoglobin regulation.
Globin synthesis was examined in mouse erythroid colonies. Bone marrow cells from DBA/2J adults were cultured in methylcellulose and labeled with 3H-leucine; globin synthesis ratios were determined following electrophoresis of lysates on polyacrylamide gels containing urea, acid, and Triton X-100. Colonies derived from the immature progenitor cells, BFU-E and CFU-E, produced close to 40% beta mi of total beta, while cluster-forming units, erythroblasts, and reticulocytes synthesized approximately 30% beta mi. Thus, beta mi synthesis decreased with increasing maturity of the erythroid compartment being examined, qualitatively resembling the decrease in fetal hemoglobin between BFU-E and erythrocytes in human adults. The mouse system described here thus provides a small animal model for studies of changes in hemoglobin expression during erythroid development.
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism*; D002478:Cells, Cultured; D004900:Erythroblasts / Q000378:metabolism; D004920:Erythropoiesis; D005260:Female; D005914:Globins / Q000096:biosynthesis*; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006454:Hemoglobins / Q000096:biosynthesis*; D007700:Kinetics; D051379:Mice; D008811:Mice, Inbred DBA; D012156:Reticulocytes / Q000378:metabolism
D006454:Hemoglobins; D005914:Globins