Accumulation of linoleic and gamma-linolenic acids in tissue lipids of pyridoxine-deficient rats.
Young male Sprague-Dawley rats were fed diets containing added pyridoxine . HCl at 22 mg/kg (control), 0 mg/kg or 88 mg/kg for 6 weeks. In comparison with control or pyridoxine-supplemented (+PN) rats, growth of the pyridoxine-deficient (-PN) rats was significantly less after 2 weeks. After 6 weeks, liver weight was higher but thymus and epididymal fat weights, in relation to body weight, were significantly lower in -PN compared to control rats. In -PN rats, phospholipid levels of linoleic and gamma-linolenic acids were increased, but arachidonic acid was decreased compared to controls in plasma, liver, thymus and skin. In liver triglycerides from -PN rats, all essential fatty acids (n3 and n6) were increased compared to both control and +PN rats. The n3 essential fatty acids were significantly increased in plasma, liver, and thymus phospholipids in the +PN compared to control rats. These results support previous reports of an effect of pyridoxine on essential fatty acid metabolism and suggest that both linoleic desaturation and gamma-linolenic acid elongation may be impaired in -PN rats. In addition, the accumulation of essential fatty acids in the liver triglycerides of -PN rats suggests that essential fatty acid turnover between triglyceride and phospholipid may be influenced by pyridoxine.
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D005234:Fatty Liver / Q000209:etiology / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008041:Linoleic Acids / Q000378:metabolism*; D008042:Linolenic Acids / Q000378:metabolism*; D008297:Male; D010743:Phospholipids / Q000097:blood / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism; D026681:Vitamin B 6 Deficiency / Q000150:complications / Q000378:metabolism*
D008041:Linoleic Acids; D008042:Linolenic Acids; D010743:Phospholipids; D014280:Triglycerides