Thioacetamide effects on protein metabolism in the liver: lessons from isolated hepatocytes.
The effects of a short-term treatment with thioacetamide have been studied in isolated hepatocytes obtained from intoxicated rats. A technique has been developed which utilizes leucine alternatively labeled with either [14C] or [3H] and permits the simultaneous evaluation of protein synthesis, catabolism and secretion in the same cell during the same incubation period. The results indicate that short-term thioacetamide treatment causes an overall slowing-down of protein metabolism. Protein synthesis, however, decreases less than protein degradation and total protein secretion; albumin secretion, which is also less than normal, seems to be less compromised than total protein secretion.
D000081:Acetamides / Q000494:pharmacology*; D000418:Albumins / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000187:drug effects*; D008297:Male; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013853:Thioacetamide / Q000494:pharmacology*; D014316:Tritium
D000081:Acetamides; D000418:Albumins; D002250:Carbon Radioisotopes; D011506:Proteins; D013853:Thioacetamide; D014316:Tritium; D007930:Leucine