Cloned mycoplasma ribosomal RNA genes for the detection of mycoplasma contamination in tissue cultures.
A cloned fragment of the mycoplasma ribosomal RNA operon was used as a molecular probe for the detection of mycoplasmas in cell cultures. According to the conditions of hybridization, the probe can detect prokaryotes in general or mycoplasmas specifically.
D003001:Cloning, Molecular; D046508:Culture Techniques; D005798:Genes, Bacterial; D006367:HeLa Cells; D006801:Humans; D009174:Mycoplasma / Q000235:genetics* / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D012335:RNA, Ribosomal / Q000235:genetics*
D012335:RNA, Ribosomal