title: Three-dimensional structure of bovine pancreatic DNase I at 2.5 A resolution.
abstract: The three-dimensional structure of bovine pancreatic deoxyribonuclease I (DNase I) has been determined at 2.5 A resolution by X-ray diffraction from single crystals. An atomic model was fitted into the electron density using a graphics display system. DNase I is an alpha, beta-protein with two 6-stranded beta-pleated sheets packed against each other forming the core of a 'sandwich'-type structure. The two predominantly anti-parallel beta-sheets are flanked by three longer alpha-helices and extensive loop regions. The carbohydrate side chain attached to Asn 18 is protruding by approximately 15 A from the otherwise compact molecule of approximate dimensions 45 A X 40 A. The binding site of CA2+-deoxythymidine-3',5'-biphosphate (Ca-pdTp) has been determined by difference Fourier techniques confirming biochemical results that the active centre is close to His 131. Ca-pdTp binds at the surface of the enzyme between the two beta-pleated sheets and seems to interact with several charged amino acid side chains. Active site geometry and folding pattern of DNase I are quite different from staphylococcal nuclease, the only other Ca2+-dependent deoxyribonuclease whose structure is known at high resolution. The electron density map indicates that two Ca2+ ions are bound to the enzyme under crystallization conditions.
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D003850:Deoxyribonuclease I / Q000032:analysis*; D046911:Macromolecular Substances; D008958:Models, Molecular; D010179:Pancreas / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D046911:Macromolecular Substances; D003850:Deoxyribonuclease I