Constraints on the models of carbohydrate metabolism in the two cell types of Dictyostelium discoideum.
The constraints in the parameters in models of the spore and stalk cells in Dictyostelium discoideum have been examined. It was found that the relative sizes of the two cellular glucose pools are not very critical, i.e. they can be varied in the models over a fairly wide range and still allow simulations which are compatible with the data. In contrast, the following model parameters are highly constrained, and must fall within narrow limits: flux through the glycogen cycle; the fraction of glycogen present which actually participates in glycogen turnover; the net rate of glycogen degradation; the concentration of exogenous labelled glucose which actually participates in cellular metabolism; the rates of exchange of this exogenous glucose with the two cellular glucose pools; the concentration of the spore glucose-6-phosphate pool, and the rate of exchange of stalk glucose-1-phosphate and stalk glucose-6-phosphate.
D050260:Carbohydrate Metabolism; D004023:Dictyostelium / Q000166:cytology / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006600:Hexosephosphates / Q000378:metabolism; D008954:Models, Biological; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
D006600:Hexosephosphates; D006003:Glycogen; D005947:Glucose; D014532:Uridine Diphosphate Glucose