title: Central nervous system features of a nicotine-resistant insect, the tobacco hornworm Manduca sexta.
abstract: The purpose of this study is to look for structural correlates of the demonstrated nicotine-insensitivity of larval Manduca sexta CNS, an insensitivity which is only slightly perturbed by desheathing (a technique used to disrupt perineurial diffusion barriers). The general organization of the hornworm ganglion is found to conform to the conventional insect pattern, but the following points are noted and discussed in terms of their potential relationship to nicotine-insensitivity: the damage caused to perineurial cells by desheathing is extremely localized, with cells immediately adjacent to the torn region showing good ultrastructural integrity; ionic lanthanum does not gain access to the subperineurial extracellular space following desheathing; lanthanum penetrates the ganglion in the cytoplasm of tracheal cells damaged peripherally during desheathing, but is excluded from the extracellular space surrounding such tracheal cells; smooth endoplasmic reticulum is much in evidence in perineurial cells and tracheal cells, sites where it might be implicated in nicotine detoxification; individual basal perineurial cells appear to cover extensive regions of the ganglion, thereby limiting intercellular diffusion.
mesh_terms: D000818:Animals; D001692:Biological Transport; D004351:Drug Resistance; D007814:Larva; D007915:Lepidoptera / Q000033:anatomy & histology*; D008854:Microscopy, Electron; D009036:Moths / Q000033:anatomy & histology* / Q000187:drug effects; D009420:Nervous System / Q000648:ultrastructure; D009538:Nicotine / Q000378:metabolism / Q000494:pharmacology
chemical_list: D009538:Nicotine