The binding of actinomycin D and Adriamycin to supercoiled DNA, single-stranded DNA and polynucleotides.
The effect of actinomycin D and adriamycin on synthetic polynucleotides, single-stranded viral DNA and supercoiled DNA has been studied employing the fluorescent probe, terbium. Marked displacement of the probe was observed when any deoxyribose-containing polynucleotide was pretreated with either drug. With supercoiled DNA, an unwinding of the supercoil was observed at very low drug concentrations (at approx. 1:500 molar ratio of drug:DNA) prior to the displacement of the terbium. This unwinding was visualized by agarose gel electrophoresis at molar ratios of approx. 1:200. The effect was more apparent and occurred at lower drug: DNA ratios with actinomycin D than with adriamycin. Unlike cis-dichlorodiammine platinum(II), actinomycin D did not protect pBR322 DNA from cleavage at its BamHI site. The hydrolysis of phi chi 174 DNA by a series of G-C-specific restriction nucleases (including HhaI, HpaII and HaeIII) was also not affected by prior treatment of the DNA with actinomycin D.
D000818:Animals; D002417:Cattle; D004277:DNA, Single-Stranded; D004278:DNA, Superhelical; D003609:Dactinomycin; D004317:Doxorubicin; D010957:Plasmids; D011068:Poly G; D011089:Polydeoxyribonucleotides; D011119:Polynucleotides; D013950:Thymus Gland
D004277:DNA, Single-Stranded; D004278:DNA, Superhelical; D011089:Polydeoxyribonucleotides; D011119:Polynucleotides; D003609:Dactinomycin; D011068:Poly G; C026628:poly(dG); C019530:poly(dC-dG); D004317:Doxorubicin