Mechanism of peroxisomal 24-hydroxylation of 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoic acid in rat liver.
We recently showed that the peroxisomal fraction of rat liver has a high capacity to catalyze conversion of 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoic acid into cholic acid and that 3 alpha,7 alpha,12 alpha,24-tetrahydroxy-5 beta-cholestanoic acid is an intermediate in this conversion. With use of 2H2O and 18O2, evidence is presented here that the formation of 3 alpha,7 alpha,12 alpha,24-tetrahydroxy-5 beta-cholestanoic acid involves intermediary formation of a delta 24-unsaturate intermediate followed by hydration.
D000818:Animals; D002458:Cell Fractionation; D019826:Cholic Acid; D002793:Cholic Acids / Q000096:biosynthesis / Q000378:metabolism*; D003903:Deuterium; D006900:Hydroxylation; D008099:Liver / Q000378:metabolism*; D008297:Male; D008830:Microbodies / Q000378:metabolism* / Q000648:ultrastructure; D010103:Oxygen Isotopes; D051381:Rats; D011919:Rats, Inbred Strains
D002793:Cholic Acids; D010103:Oxygen Isotopes; C028410:3,6,12-trihydroxycholanoic acid; D003903:Deuterium; D019826:Cholic Acid