Immunogenicity of splenocytes, associated with temporal exposition of D-galactosyl residues, in Rauscher leukemia.
To prove the existance of specific cellular immunity in leukemia the antigen preparations, obtained from the leukemia spleens and from embryos were studied in electrophoretic mobility test. It was shown that only spleens, which were obtained at days 2 and 3 after virus inoculation, possess the specific antigenic activity. Immunogenicity is associated with D-galactosyl residues, as was shown in the experiments with competitive inhibition by sugars, binding with peanut lectin and isolation of PNL-positive cells.
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology; D004586:Electrophoresis; D004622:Embryo, Mammalian / Q000276:immunology; D005690:Galactose / Q000276:immunology*; D007111:Immunity, Cellular; D037102:Lectins / Q000276:immunology; D007942:Leukemia, Experimental / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D019887:Peanut Agglutinin; D011925:Rauscher Virus; D013154:Spleen / Q000276:immunology*; D013997:Time Factors
D000951:Antigens, Neoplasm; D037102:Lectins; D019887:Peanut Agglutinin; D005690:Galactose