Direct separation of enantiomer of ephedrine and some analogues by reversed-phase liquid chromatography using (+)-di-n-butyltartrate as the liquid stationary phase.
A new chromatographic technique for the separation of enantiomers of beta-aminoalcohols is presented. A chiral complexing agent, (+)-di-n-butyltartrate (DBT), is used as liquid stationary phase, adsorbed on Phenyl Hypersil. The solutes are eluted with phosphate buffer solutions containing hexafluorophosphate as the counter ion. The resolution of the enantiomers is based on a stereoselective complex formation between DBT and the ion pairs. Separation factors of 1.1 to 1.2 are obtained for ephedrine and norephedrine. The influence of sample structure of some analogues on stereoselectivity is demonstrated.
D055598:Chemical Phenomena; D002621:Chemistry; D002853:Chromatography, Liquid / Q000379:methods; D004809:Ephedrine / Q000031:analogs & derivatives* / Q000032:analysis*; D013237:Stereoisomerism; D013644:Tartrates
D013644:Tartrates; D004809:Ephedrine