title: Vaccination of bovines against schistosomiasis japonica with highly irradiated schistosomula in China.
abstract: Vaccination of Chinese bovines (cattle and buffaloes) against schistosomiasis japonica with 36 kR gamma-irradiated schistosomula was done for laboratory challenge and for field trials in China. Altogether, 61 bovines were used. All experimental animals were vaccinated 2-3 times with 10,000 irradiated schistosomula per time. For the laboratory challenge, all experimental and control cattle were challenged with 500 normal cercariae and each buffalo, with 2,000 cercariae. The laboratory-challenged bovines were killed after 54-57 days of challenge; the bovines for the field trial in the lightly endemic area, after 5 months in the field; and the bovines for the field trial in the heavily endemic area, after 58-63 days. When the animals were killed, the number of mature worms in the vaccinated (experimental) and non-vaccinated (control) animals was recorded and the percentage of worm reduction in each group was calculated. The first group, consisting of three vaccinated and three non-vaccinated cattle, was given a laboratory challenge; the worm reduction was 71.6%. The second group, consisting of two vaccinated and three non-vaccinated buffaloes, was also given a laboratory challenge; the worm reduction was 74.4%. The third group, consisting of seven vaccinated and eight non-vaccinated buffaloes, was utilized in a field trial in a lightly endemic area; the worm reduction was 75.6%. The fourth group, consisting of eight vaccinated and nine non-vaccinated cattle, and the fifth group, consisting of nine vaccinated and nine non-vaccinated buffaloes, were pastured in a heavily endemic area. The worm reduction was 65.1% in the fourth group and 75.7% in the fifth group.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D002020:Buffaloes; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000517:prevention & control*; D002681:China; D005720:Gamma Rays; D007814:Larva / Q000528:radiation effects; D012549:Schistosoma japonicum / Q000276:immunology / Q000528:radiation effects; D012552:Schistosomiasis / Q000453:epidemiology / Q000517:prevention & control / Q000662:veterinary*; D014611:Vaccination / Q000662:veterinary*