[Ca2+ release from rat liver mitochondria subjected to deep freezing].
Freezing-thawing of rat liver mitochondria evokes a release of Ca2+ into the incubation medium containing oxidation substrates: succinate and phosphate. The release is accompanied by a decrease in the membrane potential value. ATPase and NADH-dehydrogenase inhibitors when introduced into the medium simultaneously with mitochondria prevent the both processes, but only till Ca2+ release. ADP added during Ca2+ release, initiates its uptake. When succinate is replaced by NAD-dependent substrates (malate in combination with glutamate) and in the presence of the antioxidant (ionol) Ca2+ is not released from mitochondria. It is supposed that Ca2+ release from mitochondria due to freezing-thawing is evoked by activation of lipid peroxidation and is controlled by the degree of pyridine nucleotide reduction.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D005615:Freezing; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000378:metabolism*; D051381:Rats; D012402:Rotenone / Q000494:pharmacology
D012402:Rotenone; D002084:Butylated Hydroxytoluene; D000244:Adenosine Diphosphate; D002118:Calcium
Vysvobozhdenie Ca2+ iz mitokhondriĭ pecheni krys, podvergnutykh glubokomu zamorazhivaniiu.