Structures of the oligosaccharide chains in swine trachea mucin glycoproteins.
The structures of large O-glycosidically linked oligosaccharides derived from swine trachea mucin glycoprotein were established. Reduced oligosaccharides released by treatment with alkaline borohydride were separated by gel filtration on Bio-Gel P-6 and the neutral oligosaccharides were isolated by chromatography on DEAE-cellulose. Eight oligosaccharides (DIa to BII), ranging in length from 8 to 15 sugar units, were isolated. On the basis of carbohydrate composition and analytical data from sequential treatment with exoglycosidases and permethylation analysis, the following structures were assigned to these oligosaccharides: (Formula: see text).
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D008745:Methylation; D009077:Mucins / Q000302:isolation & purification*; D009844:Oligosaccharides / Q000302:isolation & purification*; D013552:Swine; D014132:Trachea / Q000032:analysis*
D002241:Carbohydrates; D009077:Mucins; D009844:Oligosaccharides