[Partial purification and characterization of delta 7-sterol 5-desaturase from rat liver microsomes].
The terminal oxygenase of the NADH-depending lathosterol (cholest-7-en-3 beta-ol) 5-desaturase system was partially purified from rat liver microsomes, by Triton X-100 solubilization, DEAE-cellulose column chromatography, and hydrophobic affinity chromatography with Aminohexyl-Sepharose. The terminal oxygenase activity was approximately 18 fold greater than the starting microsome, and the yield was 18.4%, nevertheless, the terminal enzyme activity was almost free from other electron transfer components in microsomes. It was demonstrated that NADH, molecular oxygen, phospholipid, and three enzymes: NADH-cytochrome b5 reductase, cytochrome b5, and the terminal oxygenase, were absolutely essential for lathosterol 5-desaturation in the reconstituted system. Furthermore, the rate of the NADH-depending lathosterol 5-desaturation in the reconstitution system, was proportional to the concentration either of the terminal desaturase, cytochrome b5, or NADH-cytochrome b5 reductase, under conditions in which other enzymes were present in excess.
D000818:Animals; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D003573:Cytochrome b Group / Q000032:analysis; D015786:Cytochromes b5; D004579:Electron Transport; D008862:Microsomes, Liver / Q000201:enzymology*; D010088:Oxidoreductases / Q000032:analysis / Q000302:isolation & purification*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D051381:Rats; D012995:Solubility
D003573:Cytochrome b Group; C033144:lathosterol delta-5-dehydrogenase; D015786:Cytochromes b5; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors