Impaired neutrophil locomotion associated with hyperadhesiveness in a patient with Chédiak-Higashi syndrome.
Neutrophils from a patient with Chédiak-Higashi (CH) syndrome exhibited defective directional locomotion in a gradient of activated plasma. Further analysis of the nature of this defect showed that CH neutrophils could respond normally to stimulation with f-Met-Leu-Phe, by increasing both their motility and polarization, provided the cells were kept in suspension. Contact with the substratum resulted in the loss of both motility and polarity in the majority of cells. CH neutrophils, in contrast to normal cells, did not respond chemokinetically to f-Met-Leu-Phe. Unstimulated random locomotion of CH neutrophils was also depressed, and this correlated with increased spreading on the substratum. Our results indicate that motility, locomotion and polarisation of CH neutrophils on the substratum are depressed because of excessive adhesion.
D002448:Cell Adhesion; D002465:Cell Movement; D002609:Chediak-Higashi Syndrome / Q000097:blood*; D002634:Chemotaxis, Leukocyte; D006801:Humans; D007223:Infant; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000502:physiology*
D009240:N-Formylmethionine Leucyl-Phenylalanine