Precipitation of collagens by polyethylene glycols.
Types I, II, and III collagens are readily precipitated at neutral pH by polyethylene glycols (PEG). As the molecular weight fraction of the polyethylene glycols increases, they become more effective as precipitants on a weight basis. The amount of PEG required for precipitation depends on the pH, the ionic strength, and the nature of the buffer or salts present. In tissue culture media, low concentrations of collagens and procollagens may be quantitatively precipitated and readily collected by low-speed centrifugation. Polyethylene glycol precipitation can be used to obtain collagens and procollagens from tissue culture media at either analytical or preparative scale, and since the polyethylene glycols do not bind to collagens, the precipitates may be further analyzed directly by chromatographic or electrophoretic methods.
D002478:Cells, Cultured; D011232:Chemical Precipitation; D002850:Chromatography, Gel / Q000379:methods; D003094:Collagen / Q000302:isolation & purification*; D003470:Culture Media / Q000032:analysis; D005347:Fibroblasts / Q000032:analysis; D006801:Humans; D008970:Molecular Weight; D011092:Polyethylene Glycols; D011347:Procollagen / Q000302:isolation & purification; D012995:Solubility
D003470:Culture Media; D011347:Procollagen; D011092:Polyethylene Glycols; D003094:Collagen