title: Quantization of chloroplast DNA using dot blot filter hybridization with double-stranded probes.
abstract: Hybridization characteristics of purified chloroplast DNA, immobilized in 'dot blots' on nitrocellulose filters using radiolabeled chloroplast DNA restriction fragments or recombinant DNA probes were investigated. Conditions are described which proved a near linear relationship between amounts of hybridization and amounts of immobilized DNA. A standard curve constructed using such data provided a simple means for quantizing specific chloroplast DNA sequences in partially purified total DNA from protoplast extracts. Using this technique, DNA sequences corresponding to about 0.01% of the total immobilized DNA could be detected.
mesh_terms: D002736:Chloroplasts / Q000032:analysis*; D004247:DNA / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D010947:Plants, Toxic; D014026:Nicotiana
chemical_list: D004247:DNA