Characterization of an unscheduled DNA synthesis assay with Syrian hamster embryo cells.
The Syrian hamster embryo cell transformation assay is widely used for studies of carcinogenesis. The characterization of an unscheduled DNA synthesis (UDS) assay for these cells is reported. Benzo[a]pyrene, aflatoxin B1 and UV light induced UDS in the cells in a dose-dependent manner without exogenous metabolic activity. Nitrosopiperidine induced UDS as well as gene mutations and cell transformation only in the presence of an exogenous metabolic activation system. The utility of this UDS assay with these cells is discussed.
D000348:Aflatoxins / Q000633:toxicity; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D001711:Biotransformation; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D004622:Embryo, Mammalian; D008297:Male; D008647:Mesocricetus; D009152:Mutagenicity Tests / Q000379:methods*; D009602:Nitrosamines / Q000633:toxicity; D051381:Rats; D014466:Ultraviolet Rays
D000348:Aflatoxins; D009602:Nitrosamines; D001564:Benzo(a)pyrene; C002743:N-nitrosopiperidine; D004247:DNA