A new purification procedure for Clostridium difficile enterotoxin.
Clostridium difficile produces two toxins, an enterotoxin and a cytotoxin. The enterotoxin was purified using fast methods (tangential flow filtration, fast protein liquid chromatography). The purified enterotoxin is composed of two subunits (A1 = 41,500, A2 = 16,000) and its pI is 3.5.
D002853:Chromatography, Liquid; D003013:Clostridium / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000302:isolation & purification*; D005374:Filtration; D007525:Isoelectric Focusing; D008722:Methods
D004768:Enterotoxins