Interferon-induced proteins. Purification and characterization of a 15,000-dalton protein from human and bovine cells induced by interferon.
Human interferons induce a protein of 15,000 daltons in human and bovine cells. This protein is located in the cytoplasm in a soluble form and is induced by concentrations of interferon which induce the antiviral state. Messenger RNA prepared from interferon-treated human and bovine cells contains a mRNA which yields on translation in vitro a protein similar in size to the 15-kDa protein induced by interferon in vivo. The human protein has been purified to homogeneity from interferon-treated human cells by ion-exchange chromatography and reverse-phase high-performance liquid chromatography. A comparison of the peptides generated by V8 protease from the human and bovine 15-kDa proteins reveals that the two proteins are similar but not identical.
D000818:Animals; D002051:Burkitt Lymphoma; D002417:Cattle; D002460:Cell Line; D003609:Dactinomycin / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology / Q000502:physiology*; D007668:Kidney; D008970:Molecular Weight; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D013045:Species Specificity
D007370:Interferon Type I; D011506:Proteins; D012333:RNA, Messenger; D003609:Dactinomycin