Preparation of chromosome suspensions from cells of a solid experimental tumour for measurement by flow cytometry.
A method is described by which metaphase chromosomes are isolated from cells of a transplantable rat sarcoma. The chromosomes are derived from cells in a suspension prepared by trypsinisation of tumours from rats that have been treated with vindesine 24 h before excision in order to accumulate cells in mitosis. Histograms obtained for the chromosomes of the solid tumour are compared with flow karyotypes of cells cultured for 20 h or for several generations in vitro.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002875:Chromosomes / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D051381:Rats; D012208:Rhabdomyosarcoma / Q000032:analysis*
D004273:DNA, Neoplasm