Dispersal process associated with the L1 family of interspersed repetitive DNA sequences.
We have determined the complete nucleotide sequence for five members of the L1Md repetitive family from the beta-globin gene region of the BALB/c mouse. The five repeats are different lengths, each terminating at the 5' end at different points with respect to one another. We have analyzed the nucleotides around the endpoints of the five repeats for clues as to the mechanisms involved with the dispersal and 5' truncation of this repeat family. Each L1 member is flanked by a pair of short direct repeats. Since these direct repeats differ in length and sequence in each of the five cases, the dispersal mechanism does not involve a sequence targeted process. The sequence at the 3' end is conserved and its organization resembles the 3' end of a polyadenylated RNA, suggesting that transcripts of the repeat are involved in the dispersal process either directly or as intermediates in the generation of complementary DNA copies of the sequence. One of the L1 repeats is a recent insertion, since it is found in the Hbbd chromosome, but not in the Hbbs chromosome. This suggests a dispersal process that has been active as recently as 4 million years ago.
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA; D005914:Globins / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009876:Operon; D012319:RNA Polymerase II / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid
D005914:Globins; D004247:DNA; D012319:RNA Polymerase II