[Use of highly sensitive radioautography for determining the optimal conditions for obtaining hybridomas].
To detect the hybrid cells forming as a result of fusion of the mouse myeloma cells with the immunic mouse splenocytes, a radioautographic method was used which involved the application of 3H-hypoxanthine as a labelled precursor of nucleic acids and of high-sensitive UK emulsion for accelerated preparations of autographs. The optimal conditions of hybridization providing for the maximum yield of hybrid cells and hybrid clones in our experiments were: use of polyethylene glycol ("Löba", mol. weight 4000) in 50% (w/v) concentration, of NS-O or X-653 myeloma cells in the ratio of parental cells 1:5 (myeloma cell: splenocyte).
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D002459:Cell Fusion; D002460:Cell Line; D004655:Emulsions; D006825:Hybridomas / Q000166:cytology*; D007114:Immunization; D051379:Mice; D009101:Multiple Myeloma / Q000473:pathology; D013154:Spleen / Q000166:cytology
D004655:Emulsions
Ispol'zovanie vysokochuvstvitel'noĭ radioavtografii dlia opredeleniia optimal'nykh usloviĭ polucheniia gibridom.