A micromodification of the CH50 test for the classical pathway of complement.
A micromodification of the CH50 assay of Kabat and Mayer is described in which the total reaction volume has been reduced five-fold. This assay utilizes sheep red blood cells pooled from multiple animals rather than from a single animal to avoid screening procedures of red blood cells from individual sheep. Analysis of natural hemolysin activity in normal serum revealed a correlation with elevated CH50 titers. However, this correlation does not appear to effect the clinical interpretation of the results.
D000818:Animals; D000917:Antibody Formation; D003167:Complement Activation; D003171:Complement Pathway, Classical; D003165:Complement System Proteins / Q000032:analysis*; D004912:Erythrocytes / Q000276:immunology; D006461:Hemolysis; D006801:Humans; D008722:Methods; D012756:Sheep / Q000276:immunology
D003165:Complement System Proteins