DNA-Feulgen-cytophotometric analysis of single cells isolated from paraffin embedded tissue.
This study describes a method for the isolation of cells from paraffin embedded tissues for DNA-Feulgen-cytophotometric measurements. The relevance of the method used is demonstrated by the analysis of the DNA-distribution pattern of cells isolated from an alveolar sarcoma of the soft tissues of a 10-year old girl. The comparison between freshly prepared imprint preparations and specimens after pepsin--extraction clearly shows, that the preparation mode used did not influence the relative DNA-content. The mean values of DNA/nuclei did not show a decrease even after prolonged pepsin treatment (90 minutes). Therefore this method permits access to stored histopathological material for retrospective DNA-Feulgen-Cytophotometric investigations.
D002648:Child; D004396:Coloring Agents; D004247:DNA / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D008867:Microtomy; D010232:Paraffin; D010783:Photometry / Q000379:methods*; D012394:Rosaniline Dyes; D012509:Sarcoma / Q000032:analysis
D004396:Coloring Agents; D004273:DNA, Neoplasm; C007722:Feulgen stain; D012394:Rosaniline Dyes; D010232:Paraffin; D004247:DNA