title: Mechanical and metabolic viability of a placental perfusion system in vitro under oxygenated and anoxic conditions.
abstract: In vitro dual circuit perfusion of the placenta with well-oxygenated medium results in the continuous and stable consumption of oxygen and glucose over a 2-h perfusion period. This is reflected in a stable production of lactate and an energy charge which is higher at the end of the perfusion period than that seen in fresh placental tissue immediately after vaginal delivery. Anoxic perfusion causes an increase in glucose consumption which is more than twofold higher than that seen in the oxygenated perfusion, resulting finally in placental uptake of glucose not only from the maternal but also from the fetal circulation. Lactate production is increased during the anoxic perfusion, while the final tissue energy charge value lies between the values observed for fresh tissue and for the oxygenated perfusion. The shift to anaerobic metabolism shown by placental tissue in anoxic conditions enables continued functioning of the tissue over the 2-h perfusion period but it appears that under anoxic conditions the tissue may incur an energy debt not observed in oxygenated perfusions.
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism; D000983:Antipyrine / Q000378:metabolism; D050260:Carbohydrate Metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006801:Humans; D000860:Hypoxia / Q000378:metabolism*; D007773:Lactates / Q000096:biosynthesis; D019344:Lactic Acid; D008433:Mathematics; D009924:Organ Culture Techniques; D010101:Oxygen Consumption; D010477:Perfusion; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011773:Pyruvates / Q000096:biosynthesis; D019289:Pyruvic Acid; D013997:Time Factors
chemical_list: D000227:Adenine Nucleotides; D007773:Lactates; D011773:Pyruvates; D019344:Lactic Acid; D019289:Pyruvic Acid; D006003:Glycogen; D005947:Glucose; D000983:Antipyrine