Inhibitory effect of sterigmatocystin and 5,6-dimethoxysterigmatocystin on ATP synthesis in mitochondria.
The inhibitory effects of sterigmatocystin, O-methylsterigmatocystin, and 5,6-dimethoxysterigmatocystin on the ATP synthesis system in mitochondria were compared with that of aflatoxin B1, which disturbs the respiratory chain in mitochondria. Sterigmatocystin and 5,6-dimethoxysterigmatocystin were found to uncouple the oxidative phosphorylation process without causing depression of state 3 respiration. O-Methylsterigmatocystin did not exhibit uncoupling activity at the limited concentrations tested (due to its low solubility in an aqueous system). These compounds, as well as aflatoxin B1, elicited neither pseudo-energized nor energized swelling of mitochondria and did not inhibit Ca2+-induced swelling of mitochondria.
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D066298:In Vitro Techniques; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D009183:Mycotoxins / Q000494:pharmacology*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D051381:Rats; D013241:Sterigmatocystin / Q000031:analogs & derivatives / Q000494:pharmacology*; D014966:Xanthenes / Q000494:pharmacology*
D009183:Mycotoxins; D014966:Xanthenes; D013241:Sterigmatocystin; C043241:5,6-dimethoxysterigmatocystin; D000255:Adenosine Triphosphate; D002118:Calcium