title: Plasma catecholamines assay: comparison between fluorimetric and radioenzymatic methods.
abstract: Plasma catecholamines were simultaneously measured in duplicate plasma samples by the fluorimetric method of Renzini et al. (1970) Clin. Chim. Acta 39, 587-594) and by the radioenzymatic method of Da Prada & Zurcher ((1976) Life Sci. 19, 1161-1174). The correlation of noradrenaline and adrenaline plasma concentrations determined by the two methods were, respectively, r = 0.95 (p less than 0.001) and r = 0.75 (p less than 0.01). The fluorimetric method was less sensitive, but more economical and less time-consuming than the radioenzymatic method. The fluorimetric method is still of value for measuring plasma catecholamines in man.
mesh_terms: D002395:Catecholamines / Q000097:blood*; D004837:Epinephrine / Q000097:blood; D006801:Humans; D009638:Norepinephrine / Q000097:blood; D013050:Spectrometry, Fluorescence / Q000379:methods; D013379:Substrate Specificity
chemical_list: D002395:Catecholamines; D009638:Norepinephrine; D004837:Epinephrine