Monoclonal antibody that preferentially binds polylysine, polyarginine, and histones and selectively decorates nuclei and chromosomes.
A monoclonal antibody, designated J-57, selectively and uniformly decorates the interphase nuclei and mitotic chromosomes of a variety of eucaryotic cells as determined by indirect immunofluorescence. As determined by enzyme-linked immunosorbent assay, however, this monoclonal antibody is not monospecific. It reacts weakly with cytochrome c, RNase A, and brain tubulin. By these tests monoclonal antibody J-57 has broad cross-reactivity similar to that of antisera directed against polylysine. The differential reactions of this monoclonal antibody suggest that it may be a useful immunohistochemical probe for nuclei and chromosomes in whole cells.
D000656:Amoeba; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology*; D002478:Cells, Cultured; D002642:Chick Embryo; D002875:Chromosomes / Q000276:immunology*; D003429:Cross Reactions; D006367:HeLa Cells; D006657:Histones / Q000276:immunology*; D006801:Humans; D051379:Mice; D008938:Mitosis; D010455:Peptides / Q000276:immunology*; D011107:Polylysine / Q000276:immunology; D014404:Tubulin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006657:Histones; D010455:Peptides; D014404:Tubulin; D011107:Polylysine; C015462:polyarginine