In vitro biosynthesis of isoprene from mevalonate utilizing a rat liver cytosolic fraction.
The in vitro biosynthesis of isoprene from DL-mevalonate in the cytosolic fraction of rat liver is described. Evidence is provided suggesting a non-enzymatic formation of isoprene from isopentenyl pyrophosphate and/or dimethylallyl pyrophosphate. Furthermore, the data establish an alternate fate of the mevalonate carbon skeleton providing the first evidence that breath isoprene is linked to cholesterol biosynthesis.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001944:Breath Tests; D002070:Butadienes / Q000096:biosynthesis*; D003600:Cytosol / Q000378:metabolism; D005260:Female; D045782:Hemiterpenes; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D008297:Male; D008798:Mevalonic Acid / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism; D010420:Pentanes; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D002070:Butadienes; D045782:Hemiterpenes; D009943:Organophosphorus Compounds; D010420:Pentanes; C005059:isoprene; C043060:3,3-dimethylallyl pyrophosphate; D000255:Adenosine Triphosphate; D008274:Magnesium; D008798:Mevalonic Acid