Improvement of the rosette inhibition assay for the detection of early pregnancy factor in humans using the monoclonal antibody, anti-human-Lyt-3.
The detection of early pregnancy factor (EPF) is a controversial subject. The findings of independent groups attempting to confirm the phenomenon of EPF have been quite variable. The present article gives details of an improved rosette inhibition assay using a monoclonal antibody (anti-human-Lyt-3), instead of an anti-lymphocyte serum (ALS), in order to increase reproducibility. Results are calculated for the first time in terms of absolute monoclonal anti-Lyt-3 concentrations. It is suggested that results be expressed as the Rosette Inhibition Antibody Concentration (RIAC) or the more accurate extrapolated 25% Rosette Inhibition Antibody Concentration (25% RIAC), in place of the previously used Rosette Inhibition Titer (RIT), when monoclonal antibodies are used. Following a 1.5 h incubation period the mean 25% RIAC for pregnant serum was 15.9 +/- 4.0 ng/ml and for control serum 48.0 +/- 12.7 ng anti-Lyt-3/ml. The improved method described should enable different groups to establish the test and compare results.
D000818:Animals; D000911:Antibodies, Monoclonal; D018835:Chaperonin 10; D003080:Cold Temperature; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D007166:Immunosuppressive Agents / Q000097:blood*; D007518:Isoantibodies; D008297:Male; D010455:Peptides; D011247:Pregnancy; D011257:Pregnancy Proteins; D012397:Rosette Formation / Q000379:methods*; D012756:Sheep; D013491:Suppressor Factors, Immunologic
D000911:Antibodies, Monoclonal; D018835:Chaperonin 10; D007166:Immunosuppressive Agents; D007518:Isoantibodies; C029805:Lyt antibodies; D010455:Peptides; D011257:Pregnancy Proteins; D013491:Suppressor Factors, Immunologic; C025124:early pregnancy factor