[Analysis of polypeptides of virus-specific informosomes induced by tobacco mosaic virus and potato virus X].
Informosome-like virus-specific ribonucleoprotein (vRNP) of tobacco mosaic virus (TMV) comprise a set of four major polypeptides having molecular weights of 17 500, 31 000, 37 000 and 39 000. Of the minor polypeptides, those of apparent molecular weights 25 000, 55 000, 68 000 and 70 000 had electrophoretic mobilities of polypeptides found in a ribonucleoprotein preparation from uninoculated plants. Polypeptide with mol.wt. 175 000 is TMV coat protein so far as: a) vRNP was precipitated with immunoglobulins against TMV and TMV coat protein; b) it had electrophoretic mobility similar to mobility of TMV coat protein; c) the peptide map of polypeptides with mol.wts 31 000, 37 000 and 39 000 are probably virus-specific-products. This is supposed because they are not present in cell informosomes protein, and they are not revealed in vRNP induced in cells after infection with potato virus X (PVX). Electrophoresis of vRNP-PVX protein reveals polypeptides of 23 000 (PVX coat protein), 55 000, 70 000, 78 000, 95 000, 120 000 and 145 000.
D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D046911:Macromolecular Substances; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*; D010940:Plant Proteins / Q000032:analysis; D010942:Plant Viruses / Q000032:analysis* / Q000235:genetics; D010947:Plants, Toxic; D011506:Proteins / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D012261:Ribonucleoproteins / Q000032:analysis; D014026:Nicotiana / Q000032:analysis / Q000235:genetics / Q000382:microbiology*; D014027:Tobacco Mosaic Virus / Q000032:analysis* / Q000235:genetics; D014764:Viral Proteins / Q000032:analysis
D046911:Macromolecular Substances; D010455:Peptides; D010940:Plant Proteins; D011506:Proteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D014764:Viral Proteins; C040911:informosome
Analiz polipeptidov virusspetsificheskikh informosom, indutsiruemykh virusom tabachnoĭ mozaiki i X-virusom kartofelia.