title: Heart rate variability and electrocardiogram changes in the fetal lamb during hypoxia and beta-adrenoceptor stimulation.
abstract: Hypoxic changes in the ST waveform of the fetal electrocardiogram (FECG), with elevated T waves as the main response, have earlier been described in the fetal lamb as mediated via the beta-adrenoceptor cells, initiated by catecholamine release. A similar background to the increase in fetal heart rate variability (FHRV) during hypoxemia has been suggested. The aim of the present study was to elucidate this question and also to compare FHRV and changes in the ST waveform of the FECG as indicators of fetal distress. Twenty-six acutely exteriorized mature lamb fetuses were submitted to periods of graded hypoxemia. Fetal blood gases were measured and oxygen content was calculated. The FHRV was analyzed by a computer program and calculated as the differential index (DI) and the interval index (II). Eighty seconds of the recorded ECG coinciding in time with each fetal blood sample were analyzed using a PDP 11/40 minicomputer. The ECG signal was sampled at a rate of 1250 samples per second giving a resolution in time of 0.8 msec. Each QRS complex was detected with a semi-automatic program using a cross-correlation algorithm. QRS complexes that by the program were signalled to be doubtful were visually examined and either rejected or approved. Hence, the resulting list of R-R intervals was practically free from artefacts. From this list of R-R intervals the DI and II were computed according to YEH et al. [30]. Hypoxemia resulted in initially strongly increased DI from 3.1 +/- 2.5 to 17.7 +/- 13.8 (p less than 0.001) and in II from 1.88 +/- 0.65 to 3.77 +/- 2.06 (p less than 0.001) (Fig. 1). Obviously the change in oxygen content per se was strongly associated with the variability indices, as we found a strong correlation between delta oxygen content/min and delta DI/min (r = 0.81). In five fetuses the effect of prolonged hypoxemia on DI was studied (Fig. 2). After the initial rise from 1.6 +/- 1.1 to 31.2 /+- 9.0 (p less than 0.02) DI decreased to 19.4 +/- 20.7. A regression analysis showed a strong connection between DI and PaO2, (n = 93, T = - 5.34), whether there was no relation between DI and pH (T = - 1.85). There is strong evidence that hypoxemia and asphyxia induce an increase in the concentration of catecholamines in fetal blood [2, 23].(ABSTRACT TRUNCATED AT 400 WORDS)
mesh_terms: D000818:Animals; D004562:Electrocardiography; D005260:Female; D005318:Fetal Heart / Q000503:physiopathology*; D005311:Fetal Hypoxia / Q000097:blood / Q000139:chemically induced / Q000503:physiopathology*; D006339:Heart Rate / Q000187:drug effects*; D007545:Isoproterenol / Q000494:pharmacology*; D010100:Oxygen / Q000097:blood; D011247:Pregnancy; D012756:Sheep
chemical_list: D007545:Isoproterenol; D010100:Oxygen