title: Hypomethylation of DNA in meiotic and postmeiotic rooster testis cells.
abstract: To study whether changes in methylation of DNA are related to the structural and functional changes that chromatin undergoes throughout rooster spermatogenenis, we analyzed, by high-performance liquid chromatography, the 5-methylcytosine content of DNA purified from rooster testis cell nuclei at successive stages of the cell differentiation process. The DNA of meiotic and postmeiotic cells appears partially under-methylated, containing approximately 30% less methylcytosines than the DNA obtained from premeiotic and somatic cells.
mesh_terms: D044503:5-Methylcytosine; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D004247:DNA / Q000378:metabolism*; D008099:Liver / Q000032:analysis; D008297:Male; D008540:Meiosis; D008745:Methylation; D013091:Spermatogenesis; D013094:Spermatozoa / Q000032:analysis; D013737:Testis / Q000032:analysis / Q000166:cytology*
chemical_list: D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA