Functional deterioration of mouse liver ribosomes during aging: translational activity and activity for formation of the 47 S initiation complex.
The translational activities of ribosomes from young and old mouse livers were examined in an assay system dependent on rabbit globin mRNA. Old ribosomes showed 30-40% lower activity than young ribosomes. This observation, together with our previous findings (N. Mori, D. Mizuno and S. Goto, Mech. Ageing Dev., 10 (1979) 379-398), suggests functional deterioration of ribosomes of old animals. To examine the mechanism of the deterioration, the activities for formation of the initiation complex of 40 S ribosomal subunits in the livers of young and old mice were examined in vitro. The activity was found to be 15-20% lower in old mice (21 months old or more) than in young ones. This fact partly explains the decreased activity for ribosomal protein synthesis in old mice.
D000375:Aging; D000818:Animals; D002474:Cell-Free System; D005914:Globins / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D010442:Peptide Chain Initiation, Translational; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
D012333:RNA, Messenger; D005914:Globins