EL-4 and macrophages: generation of chemotactic monokines.
Various effects of EL-4 lymphoma supernatant fluids on macrophages were studied. Macrophages migrate under agarose to medium conditioned by the EL-4 cell line for up to 40 h. EL-4 conditioned medium after 67 h contained dialyzable migration inhibitors. In addition, upon direct treatment with the EL-4 supernatant, macrophages took on a more elongated or polarized morphology as compared to control medium or another tumour cell line conditioned medium. A 2 h treatment of macrophages with the EL-4 culture supernatant was sufficient to trigger the production and/or release of chemotactic monokines. These monokines were chemotactic for both PMNs and macrophages. In addition, a prior state of activation of the macrophage does not appear to be necessary for the production and/or release of the chemotactic monokines since resident as well as stimulated peritoneal macrophages are equally effective.
D000818:Animals; D002464:Cell Migration Inhibition; D002630:Chemotactic Factors / Q000096:biosynthesis*; D002633:Chemotaxis; D066298:In Vitro Techniques; D008223:Lymphoma / Q000276:immunology*; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D015846:Monokines; D009504:Neutrophils / Q000276:immunology; D014176:Protein Biosynthesis
D002630:Chemotactic Factors; D015846:Monokines