Rapid increase of profilin-like proteins during liver development.
Extraction of the cytoplasm of rat liver with 5% PCA and subsequent TCA (20%) treatment resulted in the recovery of only a few proteins, the predominant one having an apparent molecular weight of 12K. This 12K protein increased sharply (9-fold) between 2 weeks and 10-20 weeks and then decreased until at least 2 years after birth. 12K protein was separated into two components, 12K-1 (larger) and 12K-2 (smaller), by gel chromatography which had similar amino acid compositions to those of profilin (from Acanthamoeba and hog thyroid). Like profilin, 12K-1 protein inhibited the rate of actin polymerization, while 12K-2 protein did not. These findings suggest that liver profilin-like proteins increase sharply during development.
D000199:Actins / Q000096:biosynthesis; D000375:Aging; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003285:Contractile Proteins / Q000096:biosynthesis*; D004247:DNA / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D008840:Microfilament Proteins; D008970:Molecular Weight; D051304:Profilins; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D000199:Actins; D003285:Contractile Proteins; D008840:Microfilament Proteins; C495740:Pfn1 protein, rat; D051304:Profilins; D004247:DNA