Haematological changes induced by direct contact over a coated activated charcoal: in vivo and in vitro study.
In evaluating biocompatibility, haematological changes induced by ACCM in vivo and in vitro were studied. Twenty minutes after the start of haemoperfusion a significant fall in white blood cell number was found. After 60 minutes the leucopenia still occurred. In a previous work we suggested that complement activation is at least one of the possible mechanisms responsible for these phenomena. Moreover, our data showed a significant decrease in platelet number after 60 minutes of haemoperfusion. During the in vitro study, we found, using citrated blood, a significant correlation between aggregation response to ADP and collagen, either at different ACCM doses or at various periods of incubation. Our results suggest that the fall in platelets is due to platelet aggregation. The haemocoagulation study may have clinical importance in high-risk patients when haemoperfusion is required.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D000368:Aged; D002606:Charcoal / Q000009:adverse effects*; D003094:Collagen / Q000494:pharmacology; D005260:Female; D006464:Hemoperfusion / Q000009:adverse effects*; D006801:Humans; D007958:Leukocyte Count; D007970:Leukopenia / Q000209:etiology; D008297:Male; D008768:Methylmethacrylates / Q000009:adverse effects*; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects; D010976:Platelet Count; D013921:Thrombocytopenia / Q000209:etiology; D014511:Uremia / Q000097:blood* / Q000628:therapy
D008768:Methylmethacrylates; D002606:Charcoal; D000244:Adenosine Diphosphate; D003094:Collagen