Analysis of immunoreactive and biologically active human parathyroid hormone-peptides by high-performance-liquid-chromatography.
A combination of high-performance-liquid-chromatography (HPLC), sensitive radioimmunoassays and a homologous in vitro bioassay was used to characterise human parathyroid hormone (hPTH)-peptides in human parathyroid adenoma and plasma. Chromatography of several synthetic hPTH-peptides allows the calibration of the HPLC column. On the basis of sequence hydrophobicity the elution position of peptides can be predicted. A model for the determination of the minimal peptide sequence of each peptide has been developed which based on immunological and physico-chemical properties allows the characterisation of unknown hPTH-peptides. Using this technique the heterogeneity of circulating hPTH-peptides in human plasma has been examined. Plasma extracts from healthy individuals, osteoporotic, hyperparathyroid and pseudo-hyperparathyroid patients were investigated. A uniform pattern in the heterogeneity of hPTH-peptides was detected. Using parathyroid adenoma as reference disease specific changes were characterised.
D000236:Adenoma / Q000032:analysis*; D000595:Amino Acid Sequence; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006961:Hyperparathyroidism / Q000378:metabolism; D008954:Models, Biological; D010024:Osteoporosis / Q000378:metabolism; D010281:Parathyroid Hormone / Q000032:analysis*; D010282:Parathyroid Neoplasms / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis*; D011863:Radioimmunoassay
D010281:Parathyroid Hormone; D010446:Peptide Fragments