title: Evidence for a serotonergically mediated sympathoexcitatory response to stimulation of medullary raphe nuclei.
abstract: The cardiovascular role of serotonin (5-HT) containing neurons in the midline medullary raphe nuclei was studied in anesthetized cats. High frequency electrical stimulation of nucleus (n.) raphe (r.) pallidus, n.r. obscurus and n.r. magnus produced both pressor and depressor responses. Single shock stimulation of pressor sites produced an excitatory evoked potential of sympathetic nervous discharge (SND) recorded from the inferior cardiac nerve. Conversely, single shock stimulation of vasodepressor sites resulted in a computer-summed inhibition of SND. The mean conduction velocity in the sympathoexcitatory medullo-spinal pathway to sympathetic preganglionic neurons was calculated to be 1.24 m/s. The 5-HT antagonists methysergide and metergoline blocked the excitation of sympathetic activity evoked from medullary raphe nuclei. In contrast, these agents failed to alter the sympathoexcitatory response to electrical stimulation of lateral medulla pressor sites or the sympathoinhibitory response elicited by raphe stimulation. The 5-HT uptake inhibitor chlorimipramine increased the duration of the sympathoexcitatory response evoked from the raphe but not from the lateral medulla. Finally, mid-collicular transection did not effect the excitation of sympathetic activity elicited by stimulation of medullary raphe nuclei. These data suggest that serotonergic neurons in the midline medullary raphe nuclei provide an excitatory input to sympathetic neurons in the spinal cord.
mesh_terms: D000818:Animals; D002415:Cats; D002997:Clomipramine / Q000494:pharmacology; D004553:Electric Conductivity; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000187:drug effects; D008526:Medulla Oblongata / Q000502:physiology*; D008784:Methysergide / Q000494:pharmacology; D011903:Raphe Nuclei / Q000187:drug effects / Q000502:physiology*; D012701:Serotonin / Q000502:physiology*; D013564:Sympathetic Nervous System / Q000502:physiology*
chemical_list: D012701:Serotonin; D002997:Clomipramine; D008784:Methysergide