Cell-cycle distributions and radiation responses of Chinese hamster cells cultured continuously under hypoxic conditions.
Cell-cycle distributions were measured by flow cytometry for Chinese hamster (CHO) cells cultured continuously under hypoxic conditions. DNA histograms showed an accumulation of cells in the early S phase followed by a traverse delay through the S phase, and a G2 block. During hypoxic culturing, cell viability decreased rapidly to less than 0.1% at 120 h. Radiation responses for cells cultured under these conditions showed an extreme radioresistance at 72 h. Results suggest that hypoxia induces a condition similar to cell synchrony which itself changes the radioresistance of hypoxic cells.
D000818:Animals; D002453:Cell Cycle; D002470:Cell Survival / Q000528:radiation effects*; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000032:analysis; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry; D007399:Interphase; D010100:Oxygen; D011836:Radiation Tolerance
D004247:DNA; D010100:Oxygen