Comparison of three methods for investigating placental protein 12(PP12)-like immunoreactive substance from preovulatory follicular fluid and purified PP12 from human placenta.
Placental protein 12(PP12)-like immunoreactive fraction of preovulatory follicular fluid from Sephadex G-100 and purified placental PP12 were subjected to reverse phase high performance liquid chromatography (HPLC). The elution was carried out with acetonitrile gradient. PP12-like immunoreactivity of both samples had the same retention time. This fraction of follicular fluid from HPLC and placental PP12 were then run on a sodium dodecyl sulfate-polyacrylamide gel and transferred electrophoretically to a nitrocellulose (NC) sheet. Protein bands on the NC sheet were then detected immunologically with anti-PP12 antisera. After visualising the bands which had reacted with antisera it was obvious that PP12-like immunoreactive substance from follicular fluid and from placenta had an electrophoretic mobility corresponding to the same molecular mass.
D001826:Body Fluids / Q000032:analysis; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007120:Immunochemistry; D018970:Insulin-Like Growth Factor Binding Protein 1; D018969:Insulin-Like Growth Factor Binding Proteins; D006080:Ovarian Follicle / Q000032:analysis*; D010060:Ovulation; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis* / Q000302:isolation & purification
C032231:IGFBP1 protein, human; D018970:Insulin-Like Growth Factor Binding Protein 1; D018969:Insulin-Like Growth Factor Binding Proteins; D011257:Pregnancy Proteins; D003101:Collodion