Enzymatic reduction of synthetic polymer-bound hemin derivatives: efficient method to prepare a heme-oxygen adduct in cooled aqueous medium.
Synthetic polymer-bound hemin (iron(III) protoporphyrin IX) derivatives were effectively reduced by ferredoxin and ferredoxin-NADP reductase system. The resultant polymer-bound heme (iron(II) protoporphyrin IX) derivatives formed oxygen adducts with a lifetime of ca. 1 hr in aqueous solution at -30 degrees C. The reduction rate is discussed in terms of the structure of the hemin derivatives.
D000818:Animals; D002417:Cattle; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism*; D005288:Ferredoxins / Q000378:metabolism*; D006418:Heme / Q000031:analogs & derivatives* / Q000378:metabolism*; D006427:Hemin / Q000031:analogs & derivatives*; D008099:Liver / Q000201:enzymology; D008706:Methemoglobin / Q000378:metabolism; D008786:Metmyoglobin / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen; D011145:Polyvinyls; D013329:Structure-Activity Relationship
D005288:Ferredoxins; D011145:Polyvinyls; D008786:Metmyoglobin; D006418:Heme; D006427:Hemin; D008706:Methemoglobin; D005287:Ferredoxin-NADP Reductase; D009247:NADH, NADPH Oxidoreductases; D010100:Oxygen