Diet, nutrition intake, and metabolism in populations at high and low risk for colon cancer. In situ bromination for the separation of the cholesterol-cholestanol pair in human fecal extracts.
Neutral sterols from human feces were fractionated by thin-layer chromatography and by gas-liquid chromatography (GLC). A simple thin-layer chromatography procedure for the separation of cholestanol from cholesterol is described. This technique, involving in situ bromination of sterols on thin-layer chromatography plates, converts cholesterol and other unsaturated sterols to the corresponding dibromides. Under these conditions, the cholestanol-cholesterol pair which is normally indistinguishable on thin-layer chromatography and gas-liquid chromatography, is separated. Final quantification of the sterols was carried out by gas-liquid chromatography.
D001966:Bromine; D002777:Cholestanols / Q000032:analysis*; D002784:Cholesterol / Q000032:analysis*; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005243:Feces / Q000032:analysis*; D006801:Humans; D008722:Methods
D002777:Cholestanols; D002784:Cholesterol; D001966:Bromine