title: Iron and copper promote modification of low density lipoprotein by human arterial smooth muscle cells in culture.
abstract: Modification of low density lipoproteins by human arterial smooth muscle cells was characterized by increased electrophoretic mobility and increased content of malondialdehyde-like oxidation products reactive with thiobarbituric acid. Lipoprotein modification was promoted by micromolar concentrations of iron or copper in the culture medium and was metal ion concentration- and time-dependent. The ability of diverse media to promote smooth muscle cell-mediated low density lipoprotein modification correlated with their iron concentration. Therefore, metal ion concentration of culture media contributes substantially to low density lipoprotein modification in vitro. Human monocyte-derived macrophages took up and esterified the cholesterol from modified low density lipoprotein more extensively than from native low density lipoprotein. Metal ion-mediated modification of low density lipoprotein may be a contributing factor to the pathogenesis of arteriosclerosis.
mesh_terms: D002478:Cells, Cultured; D003300:Copper / Q000494:pharmacology; D006801:Humans; D007501:Iron / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D008670:Metals / Q000494:pharmacology*; D009000:Monocytes / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
chemical_list: D008077:Lipoproteins, LDL; D008670:Metals; D003300:Copper; D007501:Iron; D015032:Zinc