Action of a microbial glycerophospholipid:cholesterol acyltransferase on plasma from normal and LCAT-deficient subjects.
The action of a bacterial acyltransferase similar in overall reaction mechanism to the plasma enzyme lecithin:cholesterol acyltransferase (LCAT) has been studied using normal plasma and lipoproteins and plasma from LCAT-deficient patients. The microbial enzyme (GCAT) catalyzed acyl transfer using phosphatidylcholine and cholesterol in all of the lipoprotein fractions, presumably because it has no apolipoprotein cofactor. In addition, the enzyme was capable of hydrolyzing cholesteryl ester in lipoproteins but not in small unilamellar vesicles nor in micellar dispersions containing low amounts of Triton X-100. This suggests that cholesteryl ester is exposed on the surface of lipoprotein particles or that it may be transferred there quickly from the interior. Although considerable interconversion of radiolabeled cholesterol and cholesteryl ester could be demonstrated upon treatment of normal plasma or lipoproteins with the enzyme, there was little change in the actual amount of either steroid. This indicates that the rate of cholesteryl ester formation is very similar to the rate of hydrolysis. The relative proportions of cholesterol and cholesteryl ester in normal plasma are therefore near the equilibrium ratio for the reaction carried out by GCAT, or the ratio is controlled by the properties of the lipoproteins themselves. During reaction with the microbial acyltransferase, the ratio of cholesterol to cholesteryl ester in plasma from LCAT-deficient patients was reduced substantially, suggesting that the enzyme may have some practical applications.
D000217:Acyltransferases / Q000378:metabolism*; D000333:Aeromonas / Q000201:enzymology; D002784:Cholesterol / Q000097:blood; D002788:Cholesterol Esters / Q000097:blood; D006801:Humans; D007009:Hypolipoproteinemias / Q000097:blood*; D007863:Lecithin Cholesterol Acyltransferase Deficiency / Q000097:blood*; D008074:Lipoproteins / Q000097:blood; D010713:Phosphatidylcholines / Q000097:blood; D013997:Time Factors
D002788:Cholesterol Esters; D008074:Lipoproteins; D010713:Phosphatidylcholines; D002784:Cholesterol; D000217:Acyltransferases; C020597:glycerophospholipid-cholesterol acyltransferase