Reproducible preparation of a wheat germ cell-free system.
This study has examined whether the conditions used for the precipitation of plant mRNPs are suitable for the reduction of endogenous template activity in the wheat germ cell-free system. These results have led to a modification of the procedure for the preparation of the wheat germ system that allows the consistent preparation of an active in vitro translation system with low endogenous activity.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002021:Buffers; D002474:Cell-Free System; D005260:Female; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000032:analysis; D008722:Methods; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D013347:Subcellular Fractions; D014908:Triticum
D000596:Amino Acids; D002021:Buffers; D012333:RNA, Messenger; D008274:Magnesium