Intracellular glycosidases of human colon Bacteroides ovatus B4-11.
Activity of various glycosidases in the intracellular enzyme fraction of Bacteroides ovatus B4-11 was investigated. During 120 h of incubation at 37 degrees C, ca. 30% of the crude hemicellulose was hydrolyzed by an intracellular enzyme fraction of strain B4-11. Xylose was the major sugar released from crude hemicellulose. Glycosidases (alpha-1,6-glucosidase, alpha-1,4-glucosidase, beta-1,4-glucosidase, and beta-1,4-xylosidase) were induced in B. ovatus B4-11 by crude hemicellulose and heteroxylan. When B. ovatus B4-11 was grown on either crude hemicellulose or heteroxylan, the predominant enzyme in the intracellular enzyme fraction was beta-1,4-xylosidase.
D001439:Bacteroides / Q000201:enzymology*; D003106:Colon / Q000382:microbiology*; D006026:Glycoside Hydrolases / Q000032:analysis*; D006801:Humans; D011134:Polysaccharides / Q000378:metabolism
D011134:Polysaccharides; C007916:hemicellulose; D006026:Glycoside Hydrolases