[Spectral characteristics of muscle aspartyl- and valyl-tRNA- synthetases and their complexes with substrates in normal conditions and after prolonged starvation].
Spectral characteristics of aminoacyl-tRNA-synthetases (ARSases) isolated from muscles of normal rabbits and of those fasted for a long time were studied by the methods of fluorescence and differential spectroscopy. Fluorescence spectra and differential absorption spectra of the compared proteins evidenced for more hydrophobic surrounding of tryptophanyls and their less accessibility for Cs+ ions in proteins of fasted animals. Interaction of aspartyl- and valyl-tRNA-synthetases from muscles of normal and long-fasted rabbits with substrates is accompanied by the essential quenching of tryptophan fluorescence of ARSases. Equilibrium constants of substrate binding calculated from the fluorescence quenching curves are higher for specific amino acids than for non-specific ones. The effect of a long-wave shift of fluorescence spectra under marginal excitation of tryptophan residues was used to determine structural differences of enzymes in norm and under fasting and to find their structural peculiarities during formation of aminoacyl adenylate. Aminoacyl-tRNA-synthetases (ARSases) are key enzymes of the protein biosynthesis. High specificity of their interaction with substrates is the basis for the accuracy of genetic information implementation, namely translation of the genetic code. Molecular mechanisms of substrates "recognition" by ARSases are the objects of great attention of researchers.
D000604:Amino Acyl-tRNA Synthetases / Q000032:analysis*; D000818:Animals; D001226:Aspartate-tRNA Ligase / Q000032:analysis* / Q000378:metabolism; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D011487:Protein Conformation; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D013217:Starvation / Q000201:enzymology*; D013379:Substrate Specificity; D013997:Time Factors; D014364:Tryptophan / Q000032:analysis; D014637:Valine-tRNA Ligase / Q000032:analysis* / Q000378:metabolism
D014364:Tryptophan; D000604:Amino Acyl-tRNA Synthetases; D001226:Aspartate-tRNA Ligase; D014637:Valine-tRNA Ligase
Spektral'nye kharakteristiki myshechnykh aspartil- i valil-tRNK- sintetaz i ikh kompleksov s substratami v norme i posle dlitel'nogo golodaniia.