title: The effect of cooling and warming rate on cortical cell function of glycerolized rabbit kidneys.
abstract: Experiments previously reported (I.A. Jacobsen, D.E. Pegg, H. Starklint, J. Chemnitz, C.J. Hunt, P. Barfort, and M.P. Diaper, Cryobiology 19, 668, 1982) suggested that rabbit kidneys permeated with 2 M glycerol are least damaged during freezing and thawing if they are cooled very slowly (1 degree C/hr). Using similar techniques of glycerolization, cooling, storage at -80 degrees C, rewarming, and deglycerolization, active cell function in cortical tissue slices prepared from such kidneys has now been studied. Oxygen uptake, tissue K+/Na+ ratio after incubation, and slice/medium PAH ratio after incubation were measured. Kidneys cooled at 3.1 degree C/min and warmed at 4.2 degrees C/min gave poor results in the previous studies and the lowest levels of cell function in the present experiments. Kidneys cooled at 1 degree C/hr exhibited degrees of slice function that were dependent on warming rate: warming at 1 degree C/min was better than warming at either 1 degree C/hr or c.20 degrees C/min. These results refine the previously drawn conclusions, (loc cit) and indicate optimal cooling and warming rates for rabbit kidneys containing 2 M glycerol, in the region of 1 degree C/hr cooling and 1 degree C/min warming. These rates are much lower than have hitherto been used by others for any system. Some implications of these findings are discussed.
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D005615:Freezing; D005990:Glycerol; D066298:In Vitro Techniques; D007668:Kidney / Q000502:physiology*; D007672:Kidney Cortex / Q000502:physiology; D008297:Male; D009926:Organ Preservation; D010101:Oxygen Consumption; D011188:Potassium / Q000378:metabolism; D011817:Rabbits; D012964:Sodium / Q000378:metabolism; D014655:Vascular Resistance; D010130:p-Aminohippuric Acid / Q000378:metabolism
chemical_list: D012964:Sodium; D005990:Glycerol; D011188:Potassium; D010130:p-Aminohippuric Acid