A new rapid and simple assay for factor XIII activity using dansylcadaverine incorporation and gel filtration.
A new sensitive, reproducible and simple assay for factor XIII activity was developed, in which fluorescent dansylcadaverine (DC) is incorporated into casein and DC-casein is separated from free DC by gel filtration. A good correlation was found between our method and Lorand's. The main advantages of our method are: (1) our assay technique is simple with good reproducibility and takes only a couple of hours; (2) the detectable limit of factor XIII activity is about 1% of normal pool plasma, but, if necessary, can be lowered by increasing the sample amount and lengthening the reaction time.
D002103:Cadaverine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D002364:Caseins / Q000378:metabolism; D002850:Chromatography, Gel / Q000379:methods; D003959:Diamines; D005176:Factor XIII / Q000378:metabolism*; D005456:Fluorescent Dyes; D006801:Humans; D007700:Kinetics
D002364:Caseins; D003959:Diamines; D005456:Fluorescent Dyes; D005176:Factor XIII; C008542:monodansylcadaverine; D002103:Cadaverine