Nucleosome reconstruction via phosphorus mapping.
Electron spectroscopic imaging was combined with reconstruction algorithms to derive the three-dimensional structure of the nucleosome core particle to a resolution of 1.5 nanometers. Images of phosphorus distributions within individual nucleosomes were interpreted as projections of a supercoil of DNA. These were used to orient the corresponding individual nucleosome images, making it possible to reconstruct the entire nucleosome in three dimensions. The structure is consistent with known biochemical and biophysical data and explains site-specific nuclease sensitivity, although differing in part with other nucleosome models.
D004247:DNA / Q000032:analysis; D003852:Deoxyribonucleoproteins / Q000032:analysis; D006657:Histones / Q000032:analysis; D008854:Microscopy, Electron / Q000379:methods; D008961:Models, Structural; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000648:ultrastructure*; D011487:Protein Conformation; D013057:Spectrum Analysis / Q000379:methods
D003852:Deoxyribonucleoproteins; D006657:Histones; D009707:Nucleosomes; D004247:DNA