Lipase assay in duodenal juice using a conductimetric method.
Lipase activity in duodenal juice is known to undergo important variations in pathologic states, especially in cases of chronic pancreatitis. Almost all of the current assay methods are based on the measurement of hydrolysis of olive oil or triolein, mainly by potentiometry. As we have developed a conductimetric method for enzyme activity measurements, we have applied it to lipase assay. A higher experimental conductimetric sensitivity is obtained when liberated acids have a short chain (higher limiting equivalent conductivity). We have therefore used triacetin as a substrate and compared out method with potentiometry (pH-stat) and spectrophotometry. The correlation coefficients of both methods with conductimetry were 0.94 and 0.97, respectively, indicating that the conductimetric method may be used for lipase assay in duodenal juice, using triacetin as a substrate.
D001826:Body Fluids / Q000201:enzymology; D003217:Conductometry; D004386:Duodenum / Q000201:enzymology*; D006801:Humans; D008049:Lipase / Q000032:analysis* / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology; D011199:Potentiometry; D013053:Spectrophotometry; D014215:Triacetin / Q000378:metabolism
D008049:Lipase; D014215:Triacetin