title: In vitro and in situ skeletal muscle pyruvate dehydrogenase activity in adrenalectomized and glucocorticoid treated rats.
abstract: Skeletal muscle glucose oxidation is significantly reduced and alanine release enhanced in adrenalectomized rats after short-term glucocorticoid treatment. A possible site of regulation is the pyruvate dehydrogenase complex. Pyruvate dehydrogenase activity was measured in vitro in extracts of the gastrocnemius muscle of normal, adrenalectomized and short-term glucocorticoid treated rats by the p-nitroaniline-arylamine-acetyltransferase method and in situ in non-recirculating perfusions of isolated hindlimbs with physiological pyruvate levels and tracer doses of [1-14C] pyruvate by measuring the off-kinetic of 14CO2 wash out in the effluent. Neither method showed a direct influence of glucocorticoids on skeletal muscle pyruvate dehydrogenase activity.
mesh_terms: D000311:Adrenal Glands / Q000502:physiology*; D000315:Adrenalectomy; D000818:Animals; D001623:Betamethasone / Q000494:pharmacology; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009132:Muscles / Q000201:enzymology*; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005938:Glucocorticoids; D011768:Pyruvate Dehydrogenase Complex; D001623:Betamethasone