[Spectrophotometric evaluation of diene conjugates].
Primary products of lipid peroxidation in liver tissue (diene conjugates) were extracted using various systems of solvents. In the system heptane-isopropanol 1:1 the diene conjugates were mainly extracted by the alcohol. The procedure for estimation of the tissue diene conjugates involved extraction with heptane-isopropanol, purification and spectrophotometric measurement of the extinction in the alcohol fraction.
D000433:1-Propanol; D000818:Animals; D006536:Heptanes; D008054:Lipid Peroxides / Q000302:isolation & purification*; D008862:Microsomes, Liver / Q000032:analysis*; D051381:Rats; D013053:Spectrophotometry
D006536:Heptanes; D008054:Lipid Peroxides; D000433:1-Propanol
Spektrofotometricheskoe opredelenie dienovykh kon"iugatov.