Problems associated with the measurement of uric acid using two enzyme-mediated reaction systems.
We describe the problems encountered with the adaptation of two enzymatic assays for uric acid to a centrifugal analyser. A method employing the uricase/catalase/aldehyde dehydrogenase enzyme system sometimes suffered from interference due to light-scattering species in samples and reagents. The second method employing the uricase/peroxidase system gave results that were lower than the comparison method.
D000085:Acetates / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D001663:Bilirubin; D000431:Ethanol / Q000378:metabolism; D006454:Hemoglobins; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis; D008722:Methods; D009249:NADP / Q000378:metabolism; D014503:Urate Oxidase / Q000378:metabolism; D014527:Uric Acid / Q000097:blood*
D000085:Acetates; D006454:Hemoglobins; D014527:Uric Acid; D000431:Ethanol; D009249:NADP; D006861:Hydrogen Peroxide; D000444:Aldehyde Dehydrogenase; D014503:Urate Oxidase; D001663:Bilirubin