Multiple fibronectin subunits and their post-translational modifications.
We report analyses of fibronectin subunit diversity by high resolution one- and two-dimensional gel electrophoresis. We have studied plasma and cellular fibronectins of rats and hamsters. Each form of fibronectin comprises multiple distinguishable subunits and, within each rodent species, all subunits of plasma fibronectin are resolvable from those of cellular fibronectin. Some, but not all, of this heterogeneity is caused by differential glycosylation. Thus, while glycosylated plasma and cellular fibronectins share no common subunits, nonglycosylated forms of these proteins appear to share 2-3 subunits. In addition, there are subunits unique to plasma and to cellular fibronectins in both rats and hamsters, although the pattern of diversity differs slightly between species. All size variants of fibronectin are phosphorylated to varying degrees. However, only some subunits are sulfated, apparently on tyrosine residues in the C-terminal third of the molecule. Comparison of the distribution of sulfate on the various fibronectin subunits with recent results on generation of multiple mRNAs by alternative splicing suggests that tyrosine sulfate is located in a polypeptide segment present in only certain fibronectin subunits. The results reported here provide information on the likely contributions of primary sequence differences and post-translational modifications to the heterogeneity of fibronectin subunits.
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D005353:Fibronectins / Q000235:genetics* / Q000302:isolation & purification; D006020:Glycopeptides / Q000032:analysis; D008099:Liver / Q000378:metabolism*; D046911:Macromolecular Substances; D010446:Peptide Fragments / Q000032:analysis; D011499:Protein Processing, Post-Translational; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013431:Sulfates / Q000032:analysis; D013462:Sulfur Radioisotopes
D005353:Fibronectins; D006020:Glycopeptides; D046911:Macromolecular Substances; D010446:Peptide Fragments; D012333:RNA, Messenger; D013431:Sulfates; D013462:Sulfur Radioisotopes