The detection of protein p30 in seminal stains by means of thin-layer immunoassay.
The detection of p30 by means of an indirect thin-layer immunoassay (TIA) is described. Extracts from 20 samples can be analyzed in approximately 2 h with a detection limit of approximately 50 ng. The p30 protein was detected in seminal stains which had been stored at room temperature for six months and at 130 degrees C for 4 h. Blood, saliva, urine, perspiration, and tears did not interfere with the method. The reliability of the method was demonstrated in a blind study.
D005554:Forensic Medicine; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008297:Male; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D012661:Semen / Q000032:analysis*; D029589:Seminal Plasma Proteins
D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein