Inhibition of human polymorphonuclear leukocyte cell responses by ibuprofen.
The stimulation of cell swelling, cell aggregation, polymorphonuclear leukocyte locomotion, and lysosomal enzyme release in response to chemoattractant were all inhibited by ibuprofen, a nonsteroidal anti-inflammatory agent. The dosages needed to induce 50% inhibition (ID50) were 5.9, 7.6, 60, and 95 micrograms/mL, respectively. Aside from the differences in ID50, there was also a difference in the degree of maximum inhibition (Imax) of the complement C5a-stimulated responses observed, so that at achievable serum drug concentrations of 20-50 micrograms/mL, inhibition of 67-78% for cell swelling, 69-82% for cell aggregation, 20-35% for migration response, and 17-38% for lysosomal enzyme release were demonstrated. Also observed were a minor stimulatory effect on nitroblue tetrazolium reduction and an inhibitory effect on the ability to kill Staphylococcus aureus, but only at very high concentrations (approximately 2 mg/mL).
D001770:Blood Bactericidal Activity; D002449:Cell Aggregation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002630:Chemotactic Factors / Q000494:pharmacology; D003182:Complement C5 / Q000494:pharmacology; D015936:Complement C5a; D005260:Female; D006801:Humans; D007052:Ibuprofen / Q000494:pharmacology*; D066298:In Vitro Techniques; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects*; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D013211:Staphylococcus aureus / Q000502:physiology
D002630:Chemotactic Factors; D003182:Complement C5; D009580:Nitroblue Tetrazolium; D015936:Complement C5a; D007052:Ibuprofen