Hemodialysis and cell toxicity in vitro related to plasma triglycerides, post-heparin lipolytic activity and free fatty acids.
Plasma triglyceride (TG) concentrations, post-heparin lipolytic activities and the free fatty acid (FFA) pattern of uremic and normal plasma were compared and correlated to plasma toxicity as measured by the effect on human mononuclear phagocytes cultured in vitro. Plasma TG concentration and FFA: albumin molar ratios were significantly higher in uremic plasma, and a correlation was found between TG concentrations prior to heparinization and post-heparin FFA concentrations. Uremic plasma toxicity was significantly correlated to increased post-heparin FFA: albumin molar ratio. The post-heparin lipoprotein lipase activity in uremic plasma collected 120 min after heparinization was higher than in normal plasma. Qualitative and quantitative experiments in vitro showed increased plasma toxicity with increasing FFA: albumin molar ratios. The proportion of unsaturated FFA in uremic post-heparin plasma increased compared to saturated FFA during incubation for 96 hours. Altered lipid metabolism after systemic heparinization thus seems to be important for the cell toxicity of uremic plasma in vitro.
D000293:Adolescent; D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D005260:Female; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D008066:Lipolysis / Q000187:drug effects*; D008264:Macrophages / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010586:Phagocytes / Q000276:immunology; D006435:Renal Dialysis; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014280:Triglycerides / Q000097:blood*; D014511:Uremia / Q000097:blood / Q000628:therapy
D005230:Fatty Acids, Nonesterified; D014280:Triglycerides; D012710:Serum Albumin, Bovine; D006493:Heparin