Extraction and partial characterization of enkephalin-like peptides from splanchnic nerve.
A method is described for the extraction of enkephalin-like peptides from peripheral nerve using chloroform and acidic methanol to facilitate a differential extraction of peptides and lipid. Porcine splanchnic nerve contains enkephalin-like peptides in low amounts compared to porcine adrenal medulla and striatum. Gel filtration chromatography reveals the presence of enkephalin-like peptides in both processed and cryptic forms. This is the first reported isolation and partial characterization of these peptides in splanchnic nerve. The presence of these peptides in this nerve provides support for the contention that the splanchnic nerve can modulate catecholamine release from the adrenal medulla through an effect on opiate receptors located on chromaffin cells.
D000313:Adrenal Medulla / Q000032:analysis; D000818:Animals; D043424:Carboxypeptidase B; D002268:Carboxypeptidases; D002725:Chloroform; D002850:Chromatography, Gel; D003342:Corpus Striatum / Q000032:analysis; D004744:Enkephalin, Methionine / Q000032:analysis; D004745:Enkephalins / Q000032:analysis*; D000432:Methanol; D012995:Solubility; D013153:Splanchnic Nerves / Q000032:analysis*; D013552:Swine; D014357:Trypsin
D004745:Enkephalins; D004744:Enkephalin, Methionine; D002725:Chloroform; D002268:Carboxypeptidases; D043424:Carboxypeptidase B; D014357:Trypsin; D000432:Methanol