An automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c.
An automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c is described. We use a 45-min incubation in acetate buffer (pH 5.5) to eliminate labile glycated hemoglobins. In this automated system conventional modules are used but it incorporates a solvent-switching valve to select either of two buffers, which differ in pH and NaCl concentration. The chromatographic column contains "polyCAT" (a weak cation-exchanger, polyaspartic acid linked to silica). Run time is 14 min per sample. The method is precise and results correlate well with those by other ion-exchange procedures.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001322:Autoanalysis; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis*; D006455:Hemoglobins, Abnormal / Q000032:analysis; D006461:Hemolysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D011786:Quality Control; D012016:Reference Values; D013053:Spectrophotometry; D013696:Temperature; D013789:Thalassemia / Q000097:blood
D002021:Buffers; D006442:Glycated Hemoglobin; D006455:Hemoglobins, Abnormal