Anti-insulin-like (diabetogenic) peptides in pituitary extracts: role of oxytocin.
A peptide has been extracted and characterized from whole bovine pituitaries that has anti-insulin-like activities when assayed in rat adipocytes. This peptide has been purified approximately 100,000-fold, is homogeneous by thin-layer chromatography in three separate solvent systems, and shows a single peak by reverse-phase high-performance liquid chromatography. By these chemical criteria, as well as biological activity criteria (14CO2 production from D-[U-14C]glucose and D-[U-14C]glucose incorporation into glycogen in rat adipocytes], the peptide is indistinguishable from oxytocin. It reacts with anti-oxytocin antibody, and has an amino acid composition indistinguishable from purified oxytocin. The relationship between this material and other previously described anti-insulin or diabetogenic peptides is discussed, but it was not possible to conclude that this peptide, which has been purified to homogeneity and constant specific activity, is related to these previously described factors.
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D007329:Insulin Antagonists / Q000302:isolation & purification*; D008297:Male; D010121:Oxytocin / Q000494:pharmacology*; D010902:Pituitary Gland / Q000032:analysis*; D010907:Pituitary Hormones / Q000302:isolation & purification* / Q000494:pharmacology; D011863:Radioimmunoassay; D011869:Radioligand Assay; D051381:Rats
C488867:DGF protein, rat; D007329:Insulin Antagonists; D010907:Pituitary Hormones; D010121:Oxytocin; D005947:Glucose