Alterations in rat lactate dehydrogenase: age-related comparison of M- and H-LDH.
Lactate dehydrogenase (LDH: EC 1.1.1.27) of skeletal muscle and heart was purified from young and old female albino rats. Properties of both types, muscle (M-LDH) and heart (H-LDH), were compared by using enzymes purified from skeletal muscle and hearts of young (22-weeks) and old (116-weeks) rats. M-LDH showed differences with respect to properties such as thermal stability and effects of pH, urea, and sodium sulfite. However, H-LDH did not show any significant alterations in enzyme properties.
D000375:Aging; D000818:Animals; D005260:Female; D006321:Heart / Q000254:growth & development*; D007527:Isoenzymes; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D024510:Muscle Development; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase