Purification of glycogen phosphorylase from small quantities of mouse skeletal muscle.
A new approach to the purification of skeletal muscle glycogen phosphorylase is described. The purification scheme is particularly suited to preparation of the enzyme from small amounts of tissue. A combination of dye-ligand chromatography and hydrophobic chromatography yields homogenous enzyme with good recoveries. The purification is rapid and may be completed in a working day.
D000180:Acrylic Resins; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography / Q000379:methods; D004396:Coloring Agents; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008024:Ligands; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009132:Muscles / Q000201:enzymology*; D006005:Phosphorylases / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives
D000180:Acrylic Resins; D004396:Coloring Agents; D008024:Ligands; C017842:cibacron blue-sepharose; D012685:Sepharose; D006005:Phosphorylases