Glycosylated hemoglobins in heterozygotes and homozygotes for hemoglobin C with or without diabetes.
Glycosylated hemoglobin in red blood cell hemolysates of five patients homozygous for CC, 18 patients with SC condition, and 13 patients heterozygous for Hb C with or without insulin-dependent diabetes mellitus were separated by Bio-Rex 70 chromatography. The various glycosylated components were identified by analysis of the hemoglobin components for ketoamine and phosphate, in vitro glycosylation studies, and by the quantitative differences in the minor components between the participants with and without diabetes. The percentages of Hb A1a + b, Hb A1c, and Hb C1c were significantly increased in the Hb C heterozygote with diabetes. Similarly, the percentages of Hb S1a + b and Hb S1c were elevated in the SC patient with diabetes. It was noteworthy that the levels of these components became normal after adequate control of diabetes. Moreover, the levels of Hb C1c in the CC participants and Hb S1c (Hb S1c/total Hb S) in the SC patients were significantly higher than the Hb S1c levels previously reported in patients with sickle cell anemia. These findings might reflect the fact that CC and SC patients have less severe hemolytic anemia. Moreover, the relative proportions of Hb A1c and Hb C1c were nearly the same in Hb C heterozygotes, which indicated that Hb A and Hb C were glycosylated in vivo to approximately the same extent.
D000755:Anemia, Sickle Cell / Q000097:blood*; D002250:Carbon Radioisotopes; D002845:Chromatography; D006442:Glycated Hemoglobin / Q000235:genetics*; D006444:Hemoglobin C / Q000235:genetics*; D006579:Heterozygote; D006720:Homozygote; D006801:Humans
D002250:Carbon Radioisotopes; D006442:Glycated Hemoglobin; D006444:Hemoglobin C