A rapid high-performance liquid chromatographic procedure for the simultaneous determination of methionine, ethionine, S-adenosylmethionine, S-adenosylethionine, and the natural polyamines in rat tissues.
A method for the analysis of S-adenosyl-L-methionine (SAM) and S-adenosyl-L-ethionine (SAE) and their major metabolites by high-performance liquid chromatography is described. The procedure allows the simultaneous analysis of the natural polyamines, putrescine, spermidine, and spermine, and some of the major amino acids, methionine, tyrosine, and tryptophan. The uv absorbance at 254 nm is used for the determination of the SAM and SAE analogs, whereas the polyamines and amino acids are analyzed by fluorescence detection after postcolumn derivatization with o-phthalaldehyde. The method allows SAM and polyamine determinations by direct injection of the tissue extracts without prepurification. The procedure is applied to study the effects of DL-ethionine treatment on the SAM, SAE, methionine, and polyamine levels in various tissues of rats.
D000241:Adenosine / Q000031:analogs & derivatives / Q000032:analysis; D000603:Amino Acids, Sulfur / Q000032:analysis*; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D005001:Ethionine / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology; D008099:Liver / Q000032:analysis; D008297:Male; D008715:Methionine / Q000032:analysis; D010179:Pancreas / Q000032:analysis; D011073:Polyamines / Q000032:analysis*; D011467:Prostate / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012436:S-Adenosylmethionine / Q000032:analysis
D000603:Amino Acids, Sulfur; D011073:Polyamines; C012167:S-adenosylethionine; D012436:S-Adenosylmethionine; D008715:Methionine; D000241:Adenosine; D005001:Ethionine