[Study of the double-stranded fragments in the poly(A)-containing cytoplasmic RNA of the rat liver].
The double-stranded sequences in the poly(A)-containing cytoplasmic RNA (c-dsRNA) hybridize mainly with the repetitive DNA. 70-80% of double-stranded regions in the cytoplasmic poly (A)-RNA are identical to double-stranded regions of heterogeneous nuclear RNA in normal as well as in cortisone-treated rats. The thermal stability of cytoplasmic double-stranded regions is higher in the presence of polyadenylate sequences than in their absence. It is suggested that the double-stranded sequences in the poly (A) +RNA interact with poly (A) stretches and form higher order structures. The thermal stability of c-dsRNA isolated from cortisone-treated rats is higher than that from control rats.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D003348:Cortisone / Q000494:pharmacology; D003593:Cytoplasm / Q000032:analysis; D008099:Liver / Q000032:analysis; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D011061:Poly A / Q000032:analysis*; D012330:RNA, Double-Stranded / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012091:Repetitive Sequences, Nucleic Acid
D012330:RNA, Double-Stranded; D011061:Poly A; D003348:Cortisone
Issledovanie dvuspiral'nykh uchastkov v poli(A)-soderzhashchikh RNK tsitoplazmy kletok pecheni krys.