Vitamin requirements of three spiroplasmas.
A chemically defined medium (CC-494M) was used to study the vitamin requirements of three spiroplasmas representing three distinct serogroups: flower spiroplasmas [Spiroplasma floricola and FS (SR-3)] and honeybee spiroplasma [HBS (AS-576)]. Nicotinic acid and riboflavin were essential to spiroplasma growth. Nicotinamide could substitute for nicotinic acid. Populations of S. floricola, FS (SR-3), and HBS (AS-576) reached 3.2 X 10(9), 1.96 X 10(10), and 6.1 X 10(9) CFU/ml, respectively, when nicotinic acid (0.036 mg/liter) and riboflavin (0.014 mg/liter) were supplied.
D001205:Ascorbic Acid / Q000494:pharmacology; D001710:Biotin / Q000494:pharmacology; D003470:Culture Media; D009525:Niacin / Q000494:pharmacology; D012256:Riboflavin / Q000494:pharmacology; D013045:Species Specificity; D013149:Spiroplasma / Q000187:drug effects / Q000254:growth & development*; D014815:Vitamins / Q000494:pharmacology*
D003470:Culture Media; D014815:Vitamins; D009525:Niacin; D001710:Biotin; D001205:Ascorbic Acid; D012256:Riboflavin