A simple device to aid impalement of cells using conventional microelectrode drives.
Consistent penetration of cell membranes by micropipettes is facilitated by using electrode accelerators or high velocity step drives. Notwithstanding, much intracellular work is still done with conventional mechanical or hydraulic drives; cell membrane penetration is achieved by means of gentle taps on any convenient part of the set up. A remote control device is described which performs this function and is compact enough to be fixed on either the microelectrode holder or the preparation mounting. It consists of a small magnetized rod freely suspended in a pot-core coil. A current pulse through the coil jolts the rod; the inertial reaction of the coil frame provides the sudden movement required by the micropipette tip to overcome the elastic resistance of the cell membrane.
D000818:Animals; D001826:Body Fluids / Q000502:physiology*; D002347:Carps; D007424:Intracellular Fluid / Q000502:physiology*; D008564:Membrane Potentials; D008839:Microelectrodes; D009482:Neurophysiology / Q000295:instrumentation*; D012160:Retina / Q000502:physiology*