title: Identification of storage protein receptor and its precursor in the fat body membrane of Sarcophaga peregrina.
abstract: A previous paper (Ueno, K., Ohsawa, F., and Natori, S. (1983) J. Biol. Chem. 258, 12210-12214) showed that a storage protein receptor is present in membranes of the pupal fat body of Sarcophaga peregrina, whereas membranes of the larval fat body contain a cryptic receptor, and that 20-hydroxyecdysone is essential for activation of the cryptic receptor to bind and incorporate the storage protein. The present paper reports that the storage protein receptor is a membrane protein of the pupal fat body with a molecular mass of 120 kilodaltons, and that the cryptic receptor present in membranes of the larval fat body is a precursor of the active receptor with a molecular mass of 125 kilodaltons. It is suggested that when 20-hydroxyecdysone is secreted, it activates the process of conversion of the 125-kDa protein to the 120-kDa protein. Concomitant protein synthesis was not apparently necessary for activation of the storage protein receptor, suggesting that the target of 20-hydroxyecdysone in this case is the fat body membrane.
mesh_terms: D000273:Adipose Tissue / Q000032:analysis*; D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D004175:Diptera; D004441:Ecdysterone / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005216:Fat Body / Q000032:analysis* / Q000187:drug effects; D008565:Membrane Proteins / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis; D011498:Protein Precursors / Q000032:analysis*; D014357:Trypsin / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D010446:Peptide Fragments; D011498:Protein Precursors; D004441:Ecdysterone; D003513:Cycloheximide; D014357:Trypsin