Microspectrofluorometry of fluorescent photoproducts in photosensitized cells. Relationship to cellular quiescence and senescence in culture.
Microspectrofluorometry of L and WI-38 cells reveals chemical/structural changes due to quiescence or senescence, i.e., lipid peroxidation, spontaneous or photosensitized by hematoporphyrin. Cells treated with hematoporphyrin and a lysosomal umbelliferone probe show a fast-rising umbelliferone emission, plus a fluorescent photoproduct. Studies in rapidly growing versus quiescent L, early passage/late passage WI-38 cells, suggest accumulation of fluorescence Schiff bases (i.e., their association with granular regions of cells in stationary phase, spectral properties, fast increase in photosensitized cells) and a possible lysosomal membrane permeabilization in quiescent or senescent cells.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D005697:Galactosides / Q000494:pharmacology; D006415:Hematoporphyrins / Q000494:pharmacology; D006923:Hymecromone / Q000031:analogs & derivatives / Q000494:pharmacology; D007700:Kinetics; D007739:L Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D013050:Spectrometry, Fluorescence
D005697:Galactosides; D006415:Hematoporphyrins; D006923:Hymecromone; C026845:4-methylumbelliferyl-galactopyranoside