title: Dopamine 3-sulfate inhibits aldosterone secretion in cultured bovine adrenal cells.
abstract: It has been demonstrated that dopamine inhibits the aldosterone (Aldo) secretion in bovine adrenal cells in vitro. Because the majority of the total circulating dopamine (DA) in bovine plasma (72%) is present in sulfoconjugated form (6.8 +/- 3.4 pmol/ml) and free DA, DA sulfate, and phenolsulfotransferase (PST) activity can be found in the bovine adrenal cortex, we examined whether the inhibitory action of free DA on angiotensin II- (ANG II) stimulated Aldo secretion by cultured bovine adrenal cells can also be reproduced by sulfoconjugated DA analogues. Cells from the bovine adrenal glands cultured for 3 days before testing responded to 10(-8) M ANG II stimulation by a 10- to 12-fold increase in Aldo output. This stimulatory effect was partially inhibited by the addition of free DA and DA 3-sulfate, whereas DA 4-sulfate was inactive. Both free DA and DA 3-sulfate produced their maximal inhibitory effect at a concentration of 100 microM, with a decrease in Aldo secretion by 61% (free DA) and 44% (DA 3-sulfate). The slightly different inhibition curves for DA and for DA 3-sulfate on Aldo secretion cannot be explained by liberation of free DA through hydrolytic processes because less than 1% free DA was detected after the incubation of the cells with sulfoconjugated DA. These in vitro findings suggest that DA 3-sulfate may be effective in complementing the dopaminergic inhibition of Aldo secretion at the adrenal level.
mesh_terms: D000311:Adrenal Glands / Q000166:cytology / Q000378:metabolism*; D000450:Aldosterone / Q000378:metabolism*; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004298:Dopamine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D000451:Mineralocorticoid Receptor Antagonists / Q000494:pharmacology; D013268:Stimulation, Chemical; D014018:Tissue Distribution
chemical_list: D000451:Mineralocorticoid Receptor Antagonists; D000804:Angiotensin II; C022807:dopamine 4-O-sulfate; D000450:Aldosterone; C007558:dopamine 3-O-sulfate; D004298:Dopamine