Determination of HDL-cholesterol using 2,4,6-tribromo-3-hydroxybenzoic acid with a commercial CHOD-PAP reagent.
The Boehringer one-component high-performance cholesterol oxidase reagent has been modified by the inclusion of 2,4,6-tribromo-3-hydroxybenzoic acid (TBHBA) to give a fourfold increase in sensitivity to a molar absorbance of approximately equal to 29,000 with respect to cholesterol. The resulting reagent system is particularly suitable for the determination of plasma HDL-cholesterol for which a reagent of high sensitivity is required. A simple method of bromination avoiding the use of elemental bromine is used to prepare TBHBA. The modified reagent system has been found to have good within- and between-batch precision and has shown itself to be reliable and trouble-free.
D001970:Bromobenzoates; D002789:Cholesterol Oxidase; D008076:Cholesterol, HDL / Q000097:blood*; D006801:Humans; D062385:Hydroxybenzoates; D007202:Indicators and Reagents; D008722:Methods; D010544:Peroxidases; D002787:Sterol Esterase
D001970:Bromobenzoates; D008076:Cholesterol, HDL; D062385:Hydroxybenzoates; D007202:Indicators and Reagents; C043713:2,4,6-tribromo-3-hydroxybenzoic acid; D002789:Cholesterol Oxidase; D010544:Peroxidases; D002787:Sterol Esterase