Purification of swine kidney alkaline phosphatase by immunoaffinity chromatography.
A homogeneous alkaline phosphatase preparation was obtained from swine kidney cortex by a simple purification step of immunoaffinity chromatography. The enzyme was purified 426 times that of the initial acetone powder with a recovery of 69.6% and a specific activity of 1206 units/mg of protein. The sodium dodecyl sulfate-gel electrophoretic pattern showed a single 80,000-Mr protein band as the monomer of the purified enzyme.
D000469:Alkaline Phosphatase / Q000302:isolation & purification*; D000818:Animals; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D007120:Immunochemistry; D007668:Kidney / Q000201:enzymology*; D013552:Swine
D000469:Alkaline Phosphatase