Preservation of red cell antigens during storage of blood with different anticoagulants.
A major concern when storing blood is that the antigens of the red cells will lose some of their reactivity in vitro. As part of a study of blood stored in newer anticoagulants, we tested the persistence of the ABO, Rh, Lewis, and P antigens. Blood was stored for 21 to 35 days in several anticoagulant-preservative systems, such as citrate-phosphate-dextrose and several additive solutions, while using bags of polyvinyl chloride plasticized with di(2-ethyl hexyl) phthalate or tri (2-ethyl hexyl) trimellitate. No significant loss of antigenicity occurred in the ABO and Rh systems, but antigens in the Lewis and P systems lost some reactivity during storage.
D000925:Anticoagulants / Q000494:pharmacology*; D001793:Blood Preservation; D004910:Erythrocyte Membrane / Q000187:drug effects; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D010968:Plasticizers; D013997:Time Factors
D000925:Anticoagulants; D010968:Plasticizers