Automated continuous measurement of erythrocyte volume under isotonic conditions.
The time course of volume changes of erythrocytes, suspended in isotonic solutions containing permeable substances, was studied using a cell monitoring system which was assembled on the basis of Coulter's principle. For comparison, the hematocrit method was employed. The data obtained demonstrated that urea, glycerol, ethanol, and methanol induced a rapid volume increase, whereas glucose and galactose induced a slow volume increase. These results correspond well to the data obtained by the hematocrit method. The present method, which requires only a minimum volume of blood, showed high accuracy and high reproducibility. Thus, the method was considered applicable to experimental and clinical studies of membrane permeability of erythrocytes.
D002463:Cell Membrane Permeability / Q000187:drug effects; D004909:Erythrocyte Indices / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology; D005690:Galactose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D006400:Hematocrit; D006801:Humans; D007552:Isotonic Solutions; D000432:Methanol / Q000494:pharmacology; D008722:Methods; D014508:Urea / Q000494:pharmacology
D007552:Isotonic Solutions; D000431:Ethanol; D014508:Urea; D005947:Glucose; D005990:Glycerol; D005690:Galactose; D000432:Methanol