title: The determination of sex from forcibly removed hairs.
abstract: The determination of sex from forcibly removed hairs in forensic science laboratories has, in the past, been based almost entirely on the presence or absence of the Y chromosome in the cells of the hair root sheath. Since the human male genotype is XY and the female is XX, a technique was devised that permits root sheath cells to be stained sequentially for the Y and then the X chromosome using quinacrine mustard. Following staining, the Y and the X chromosome fluorescence were observed, at pH 5.5 and 3.0, respectively, by epifluorescence. The X and Y chromosome counts obtained for a single hair root specimen were reported as a Y-X (Y minus X) score. The results reported show that specimens from males gave positive Y-X score while specimens from females gave negative Y-X scores. Results of an age study and blind trials were also reported.
mesh_terms: D005260:Female; D006197:Hair / Q000032:analysis*; D006801:Humans; D008297:Male; D011797:Quinacrine Mustard; D012730:Sex Chromosomes / Q000032:analysis; D012732:Sex Determination Analysis; D014960:X Chromosome / Q000032:analysis; D014998:Y Chromosome / Q000032:analysis
chemical_list: D011797:Quinacrine Mustard