Incorporation of [18O]water into 4-hydroxybenzoic acid in the reaction of 4-chlorobenzoate dehalogenase from pseudomonas spec. CBS 3.
4-Chlorobenzoate is dehalogenated by 4-chlorobenzoate dehalogenase from Pseudomonas spec. CBS 3 to form 4-hydroxybenzoate. In 18O enriched water the hydroxygroup of 4-hydroxybenzoate is quantitatively labelled with 18O. This result clearly shows, that 4-chlorobenzoate dehalogenase catalyzes the hydrolytic cleavage of the halogen-carbon bond, without the involvement of molecular oxygen, a reaction not yet described.
D002849:Chromatography, Gas; D006867:Hydrolases / Q000378:metabolism*; D062385:Hydroxybenzoates / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods; D007700:Kinetics; D013058:Mass Spectrometry; D010103:Oxygen Isotopes; D010226:Parabens; D011549:Pseudomonas / Q000378:metabolism*; D014867:Water / Q000378:metabolism
D062385:Hydroxybenzoates; D010103:Oxygen Isotopes; D010226:Parabens; D014867:Water; D006867:Hydrolases; C042846:4-chlorobenzoate dehalogenase; C038193:4-hydroxybenzoic acid