title: Antigenicity of mouse interferons induced in vitro and in vivo by poly(I).poly(C).
abstract: Various mouse IFNs induced by poly(I).poly(C) or poly(ICLC) were analyzed for the antigenic types by neutralization tests using anti-IFN-alpha and anti-IFN-beta antibodies. The IFN samples included IFNs produced by L cells, by spleen cells in vitro and ex vivo, and in plasma of mice injected with the inducers. L-cell IFN was estimated to consist of IFN-beta and IFN-alpha at a ratio of about 80:20. Spleen IFNs also consisted of both IFN species, but IFN-alpha was the major component. Blood IFN samples obtained 1-4 h after injection of poly(I).poly(C) or poly(ICLC) consisted of the two molecular species, whereas the samples 8 and 24 h after injection contained only IFN-alpha. High levels of IFN activity (IFN-alpha) persisted much longer in the circulation of mice injected with poly(ICLC) than in mice injected with poly(I).poly(C).
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000096:biosynthesis / Q000276:immunology*; D007739:L Cells / Q000276:immunology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011070:Poly I-C / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology
chemical_list: D000906:Antibodies; D000941:Antigens; D007370:Interferon Type I; D011070:Poly I-C