Isolation of Chinese hamster ovary cells that overproduce asparaginyl-tRNA synthetase.
Temperature-resistant revertants, derived from the temperature-sensitive CHO asparaginyl-tRNA synthetase mutant, Asn-5, were isolated and characterized. Several lines of evidence indicate that the temperature-resistant phenotype of the revertants is due to their overproducing the same altered enzyme present in the Asn-5 parent.
D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D001226:Aspartate-tRNA Ligase; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D007700:Kinetics; D008970:Molecular Weight; D009154:Mutation; D010053:Ovary; D012346:RNA, Transfer, Amino Acyl; D013696:Temperature; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity
D012346:RNA, Transfer, Amino Acyl; D000604:Amino Acyl-tRNA Synthetases; D001226:Aspartate-tRNA Ligase; C029194:asparaginyl-tRNA synthetase; D013755:Tetradecanoylphorbol Acetate