Methylating and ethylating carcinogens have different requirements for poly(ADP-ribose) synthesis during malignant transformation.
Transformation of mouse C3H 10T1/2 cells by various alkylating carcinogens can be modulated by inhibiting poly(ADP-ribose) synthesis with a low concentration of 3-amino-benzamide, which induces no additional toxicity or reported side effects. Transformation by methylating agents was decreased by 3-aminobenzamide, whereas transformation by ethylating agents was increased. These results confirm earlier work on transformation by methylating agents, X-rays and u.v. light. Transformation by ethylating agents, however, appears to proceed by a different mechanism.
D000477:Alkylating Agents / Q000633:toxicity*; D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002273:Carcinogens; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000378:metabolism; D004260:DNA Repair; D008745:Methylation; D008769:Methylnitronitrosoguanidine; D051379:Mice; D008809:Mice, Inbred C3H; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis*; D011064:Poly Adenosine Diphosphate Ribose / Q000096:biosynthesis*
D000477:Alkylating Agents; D001549:Benzamides; D002273:Carcinogens; D009702:Nucleoside Diphosphate Sugars; D008769:Methylnitronitrosoguanidine; D011064:Poly Adenosine Diphosphate Ribose; C025160:3-aminobenzamide