Isolation of human erythrocyte membranes in glucose solution.
A method is described for the preparation or removal of erythrocyte membranes from hemolysates by a glucose solution. The procedure is simple and rapid, requiring centrifugation at 8000g for 2 min. The preparation has microscopic shape and two-dimensional peptide patterns similar to those of the membrane isolated by conventional procedures (10,000g for 20 min). The present procedure is suitable for dealing with a bulky preparation or for removal of erythrocyte membranes from large volumes of hemolysates to purify enzymes and proteins of soluble or membrane fractions.
D002458:Cell Fractionation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D004910:Erythrocyte Membrane / Q000032:analysis; D005947:Glucose; D006461:Hemolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007552:Isotonic Solutions; D010455:Peptides / Q000097:blood; D012995:Solubility
D007552:Isotonic Solutions; D010455:Peptides; D005947:Glucose