Preparation of chloroplast DNA from pea plastids isolated in a medium of high ionic strength.
A simple, rapid, and inexpensive method for the preparation and purification of chloroplast DNA (cpDNA) from pea has been developed. The crucial step is the isolation of chloroplasts in a medium of high ionic strength (I congruent equal to 1.40 M). CpDNA from pea prepared according to this method has successfully been used for restriction enzyme mapping, Southern transfers, and cloning.
D002736:Chloroplasts / Q000032:analysis*; D002845:Chromatography / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D007887:Fabaceae / Q000032:analysis*; D009994:Osmolar Concentration; D010946:Plants, Medicinal
D004247:DNA