Negative evidence for an adaptive response to lethal and mutagenic effects of alkylating agents in V79 Chinese hamster cells.
V79 Chinese hamster cells were subjected to a number of different pretreatment regimens with low concentrations of N-methyl-N-nitrosourea (MNU), N-ethyl-N-nitrosourea (ENU) or N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). They were then treated with either MNU or ENU at toxic and mutagenic concentrations. No differences in cell killing or mutation induction to 6-thioguanine resistance were found between pretreated and non-pretreated cells. These results argue against the existence of an adaptive response to alkylating agents in this cell line.
D000477:Alkylating Agents / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004260:DNA Repair / Q000187:drug effects*; D004334:Drug Administration Schedule; D005260:Female; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D009154:Mutation / Q000187:drug effects*; D010053:Ovary
D000477:Alkylating Agents; D007041:Hypoxanthine Phosphoribosyltransferase