title: Effects of metals and organic compounds on hepatic glutathione, cysteine, and acid-soluble thiol levels in mullet (Mugil cephalus L.).
abstract: The hepatic acid-soluble thiol content of striped mullet (Mugil cephalus) exposed to cadmium (10 mg/liter seawater), mercury (200 micrograms/liter), fluorene (100 micrograms/liter), dibenzofuran (750 micrograms/liter), or a 20% water-soluble fraction of a No. 2 fuel oil, and of winter flounder (Pseudopleuronectes americanus) exposed to pentachlorophenol (200 micrograms/liter) for up to 3 weeks was determined. Exposure to these chemicals caused an elevation of hepatic acid-soluble thiol content over control values. Similarly, the acid-soluble thiol content was 1.75 times control values 24 hr after injection with acetaminophen (40 mg/100 g body wt, ip). In contrast, hepatic acid-soluble thiol concentrations did not fluctuate after feeding or during short-term starvation, or after acute or chronic physical trauma. Thus, the increase in hepatic acid-soluble thiols observed in mullet appears to be a specific response to chemical insult. The effect of chemical exposure on specific thiols can vary with the compound under investigation. Glutathione accounted for most of the acid-soluble thiol increase observed in mullet exposed to oil, whereas other acid-soluble thiols were also elevated after exposure to cadmium. Injection of [14C]glycine into cadmium- and oil-treated mullet showed that hepatic uptake of this amino acid substrate and its incorporation into hepatic glutathione increased after chemical exposure. The proportion of the total [14C]glycine in the liver incorporated into glutathione was unchanged after oil exposure, but was significantly increased in cadmium-exposed fish. These results suggest that chemicals may elevate glutathione content by enhancing the hepatic uptake of amino acid substrates and also the activity of biosynthetic enzymes.
mesh_terms: D000082:Acetaminophen / Q000494:pharmacology; D000818:Animals; D001572:Benzofurans / Q000494:pharmacology; D002104:Cadmium / Q000494:pharmacology; D003545:Cysteine / Q000032:analysis*; D005399:Fishes / Q000378:metabolism*; D005449:Fluorenes / Q000494:pharmacology; D005508:Food Deprivation; D005648:Fuel Oils; D005978:Glutathione / Q000032:analysis*; D005998:Glycine / Q000494:pharmacology; D008099:Liver / Q000032:analysis / Q000187:drug effects*; D008628:Mercury / Q000494:pharmacology; D010416:Pentachlorophenol / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D001572:Benzofurans; D005449:Fluorenes; D005648:Fuel Oils; D013438:Sulfhydryl Compounds; D002104:Cadmium; D000082:Acetaminophen; C041509:fluorene; C023614:dibenzofuran; D010416:Pentachlorophenol; D008628:Mercury; D005978:Glutathione; D003545:Cysteine; D005998:Glycine