Polymorphic acetylation of 7-amino-clonazepam in human liver cytosol.
The N-acetylation of the reduced metabolite of clonazepam 7-amino-clonazepam was studied in cytosolic preparation from human fetal and adult livers. The metabolite formed 7-acetamido-clonazepam was measured with high performance liquid chromatography. A bimodal distribution of the N-acetyltransferase activities was observed in cytosols from human adult livers. These activities were 117 +/- 11 and 27 +/- 16 pmoles X mg-1 X min-1 for rapid and slow acetylators, respectively. The data observed in the fetal specimens did not allow any conclusion about bimodality because of a low number of samples.
D000107:Acetylation; D001570:Benzodiazepinones / Q000378:metabolism*; D002998:Clonazepam / Q000031:analogs & derivatives / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D005260:Female; D005865:Gestational Age; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000196:embryology / Q000378:metabolism*; D011247:Pregnancy; D012907:Smoking
D001570:Benzodiazepinones; C020860:7-aminoclonazepam; D002998:Clonazepam