The use of pig mononuclear cells to inhibit pulmonary tumor formation in isogenic mice.
A-strain mice received 1 X 10(5) A-strain mammary carcinoma, B72, cells iv on Day 0. At the same time fragments of the same tumor were implanted into the ileal mesentery of a pig together with fragments of cellulose sponge. The middle third of the pig mesenteric lymph node chain was resected. Sponge fragments of equivalent size, shape, and total weight were implanted alone into the jejunal mesentery. Seven days later, separate cell suspensions were made from the (disconnected) proximal (nonimmune) and distal (tumor-immune) segments of lymph node chain. Cells were also expressed by digital pressure from the sponges removed from the jejunal (nonimmune) and ileal (immune) segments of mesentery. A comparison was made between the antitumor action of immune lymph node cells and the two categories of sponge cell. Cells (4 X 10(6] were injected iv into the mice on Day 7 and the number of pulmonary tumors counted after killing the mice on Day 14. Cells obtained from the "immune" sponges were significantly more effective in reducing the number of pulmonary tumors than cells from the "nonimmune" sponge, or from the immune lymph nodes. The antitumor action of all cell suspension was abolished when 4 X 10(6) cells from the "interface" layer, following centrifugation on a Ficoll-triosil column, were injected into the mice. Antitumor activity was found to be correlated with the presence of "blast" cells in the pig mononuclear cell suspensions.
D000818:Animals; D002469:Cell Separation; D007958:Leukocyte Count; D008175:Lung Neoplasms / Q000628:therapy*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008325:Mammary Neoplasms, Experimental / Q000628:therapy; D008643:Mesentery / Q000166:cytology; D051379:Mice; D008805:Mice, Inbred A; D009000:Monocytes / Q000502:physiology*; D009369:Neoplasms / Q000276:immunology; D013552:Swine