Different aggregation properties of sea urchin embryonic cells at different developmental stages. I. Stage specificity of aggregation factors solubilized by butanol.
Surface proteins solubilized with butanol from purified plasma membranes of sea urchin embryos at different developmental stages were tested for their aggregation promoting activity on dissociated cells. Cells used for the assays were obtained either from blastulae or from embryos at the 16 cell stage. Results show that a strong enhancement of cell aggregation was produced only when extracted proteins and dissociated cells were obtained from embryos at the same developmental stage.
D000818:Animals; D001755:Blastocyst / Q000032:analysis / Q000187:drug effects; D000440:Butanols; D015815:Cell Adhesion Molecules; D002449:Cell Aggregation / Q000187:drug effects*; D002462:Cell Membrane / Q000032:analysis; D008565:Membrane Proteins / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D012617:Sea Urchins / Q000187:drug effects / Q000196:embryology*; D012997:Solvents
D000440:Butanols; D015815:Cell Adhesion Molecules; D008565:Membrane Proteins; D011506:Proteins; D012997:Solvents; C013065:cell aggregation factors