Megakaryocytopoiesis in bone marrow-derived stromal-hemopoietic cells co-cultures: action of Tamm-Horsfall glycoprotein.
Murine bone marrow cells are cultured in agar in the presence of marrow-derived fibroblasts and pokeweed-stimulated spleen conditioned medium. Granulo-monocytic colonies and a few megakaryocytic and mixed colonies are induced. The addition of Tamm-Horsfall glycoprotein - a human glycoprotein produced in the kidneys - increases the number of colonies and stimulates megakaryocytopoiesis. The glycoprotein does not have colony-stimulating activity nor does it induce megakaryocytopoiesis in monolayer cultures. Its major glycopeptide retains the ability to increase the number of colonies, but not to induce megakaryocytopoiesis. Cell-cell interactions occurring in the hemopoietic microenvironment and the effect of glucides are discussed.
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells; D002450:Cell Communication; D002454:Cell Differentiation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000502:physiology; D006410:Hematopoiesis; D008533:Megakaryocytes / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009088:Mucoproteins / Q000502:physiology*; D058949:Uromodulin
D009088:Mucoproteins; C548612:UMOD protein, human; C548613:Umod protein, mouse; D058949:Uromodulin; D000110:Acetylcholinesterase