title: Nonspecific cytotoxic cells in fish (Ictalurus punctatus). III. Biophysical and biochemical properties affecting cytolysis.
abstract: Nonspecific cytotoxic cells (NCC) from the catfish (Ictalurus punctatus) may comprise a population of cells that are responsible for cellular immunity in the fish. NCC kill a wide variety of transformed target cells, and previous studies have indicated that NCC share properties with mammalian natural killer cells. In the present study, many biophysical and biochemical properties of NCC were defined. NCC were nylon wool nonadherent and adherent. NCC activity was also enriched in plastic nonadherent cells. NCC were nonphagocytic (for carbonyl iron), and they did not bind to Sephadex G-10. Characterization of NCC by density gradient centrifugation indicated that they comprise a relatively homogenous population of cytolytic cells that band at 45.5% Percoll. Moderate to high doses (500-2500 R) of X-irradiation produced a stimulatory effect on NCC lysis of labeled target cells. Additional studies indicated that a soluble suppressor protein in catfish serum (CFS) regulated NCC activity. This S. aureus protein A binding component isolated from CFS suppressed NCC activity. Analysis by SDS-PAGE indicated that the soluble regulatory protein had properties similar to immunoglobulin. These data indicate that NCC share some biophysical properties with mammalian natural killer cells. In addition, NCC appear to be under partial cell regulation by a radiation sensitive suppressor cell and also by a soluble regulator serum immunoglobulin component.
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002469:Cell Separation; D003165:Complement System Proteins; D003602:Cytotoxicity, Immunologic / Q000528:radiation effects; D005260:Female; D005399:Fishes / Q000033:anatomy & histology / Q000276:immunology*; D007136:Immunoglobulins; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D008322:Mammals / Q000276:immunology; D010586:Phagocytes / Q000276:immunology
chemical_list: D007136:Immunoglobulins; D003165:Complement System Proteins