The application of reversed-phase high-performance liquid chromatography to in vitro drug metabolism studies with N-alkylarylamines.
Analytical procedures have been investigated for the separation, detection, identification and quantitation of some metabolites of N-benzyl-4-substituted anilines. Techniques based on gas-liquid chromatography were investigated and found to be unsatisfactory. By the use of reversed-phase high-performance liquid chromatography with gradient and ion-pairing techniques, methods were devised for the simultaneous analyses of a variety of metabolites. The method involves minimum sample work-up (acetonitrile precipitation) and allows easy and prompt analysis in biological media avoiding undue decomposition of unstable metabolites.
D000588:Amines / Q000032:analysis*; D000814:Aniline Compounds / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003640:Dealkylation; D006863:Hydrogen-Ion Concentration; D006900:Hydroxylation; D010084:Oxidation-Reduction
D000588:Amines; D000814:Aniline Compounds