title: Glycolipids of the mouse peritoneal macrophage. Alterations in amount and surface exposure of specific glycolipid species occur in response to inflammation and tumoricidal activation.
abstract: We have characterized the major glycolipid constituents of the mouse peritoneal macrophage, and have demonstrated that alterations in the amount and in the accessibility of specific glycolipid species to galactose oxidase/NaB3H4 labeling, an indicator of glycolipid surface exposure, occur in response to inflammation and as a consequence of activation to a tumoricidal state. The key findings are: (a) Asialo GM1, a major neutral glycolipid constituent of all macrophage populations examined, is accessible to galactose oxidase/NaB3H4 labeling on the surface of TG-elicited and BCG-activated macrophages but not on resident macrophages; (b) GM1 is the predominant ganglioside constituent of the mouse macrophage. Resident macrophages contain two distinct GM1 species, as determined by cholera toxin binding, while TG-elicited and BCG-activated macrophages contain an additional GM1 species. Differences in the relative amounts of these GM1 species, as well as in their accessibility to galactose oxidase/NaB3H4 labeling, exist among the macrophage populations. These observations suggest that both a chemical and spatial reorganization of surface glycolipids occurs in response to inflammation and tumoricidal activation.
mesh_terms: D000818:Animals; D015703:Antigens, CD; D001202:Ascitic Fluid / Q000276:immunology; D005260:Female; D005677:G(M1) Ganglioside / Q000302:isolation & purification; D005699:Galactosylceramides / Q000302:isolation & purification; D006017:Glycolipids / Q000302:isolation & purification / Q000378:metabolism*; D006028:Glycosphingolipids / Q000302:isolation & purification; D007249:Inflammation / Q000276:immunology*; D007790:Lactosylceramides; D007942:Leukemia, Experimental / Q000276:immunology; D008262:Macrophage Activation; D008264:Macrophages / Q000145:classification / Q000276:immunology / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D015703:Antigens, CD; D005699:Galactosylceramides; D006017:Glycolipids; D006028:Glycosphingolipids; D007790:Lactosylceramides; D008563:Membrane Lipids; D005677:G(M1) Ganglioside; C009744:CDw17 antigen; C018835:asialo GM1 ganglioside