title: Effect of sodium selenite on the ciliary activity, adenosine triphosphate, and protein synthesis in mouse trachea organ cultures.
abstract: Trachea from albino mice were cut transversely into nearly identical rings and incubated in medium 199 with Hanks salts and HEPES buffer at 37 degrees C. Sodium selenite at 0.5-5 mM depressed the ciliary activity. With 1 and 5 mM sodium selenite, a 50% reduction in the activity index was observed after approximately 5 and 1.5 h, respectively. The ATP content in trachea rings was reduced with 0.05-5 mM sodium selenite, and increasing concentrations gave decreasing amount of ATP after incubation for 4 and 21 h. The rate of protein synthesis as determined by incorporation of radioactive leucine was reduced with 0.5 and 2 mM sodium selenite. The synthesis was reduced quickly by 2 mM sodium selenite, which gave a 30% reduction after incubation for 1 h. It seems that the ATP levels may be used as the most sensitive indication of sodium selenite toxicity in mouse trachea.
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D002923:Cilia / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D009924:Organ Culture Techniques; D014176:Protein Biosynthesis; D020887:Selenious Acid; D012643:Selenium / Q000633:toxicity*; D014132:Trachea / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000255:Adenosine Triphosphate; D020887:Selenious Acid; D012643:Selenium