Uptake and distribution of exogenous serum proteins in the cultured rat embryo.
Head fold stage rat embryos were cultured on a medium consisting of adult rat serum for 48 hr. During the final 10 or 20 hr of culture [35S]-methionine was added to the medium. Sera from visceral yolk sac blood vessels, exocoelomic cavity fluid, and amniotic sac fluid were analyzed by SDS-polyacrylamide gel electrophoresis followed by Amido Black staining and fluorography. Amido Black staining showed bands in all three embryonic fluid compartments which migrated at rates comparable to transferrin, alpha-fetoprotein, albumin, IgG, and hemoglobin. Fluorography, however, showed that radioactivity was restricted to alpha-fetoprotein, IgG, and hemoglobin. This suggested that at least some transferrin and albumin were transferred intact from the exogenous medium to the embryonic fluid compartments.
D000653:Amniotic Fluid / Q000378:metabolism; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008715:Methionine; D008722:Methods; D051381:Rats / Q000196:embryology* / Q000378:metabolism; D011919:Rats, Inbred Strains; D013462:Sulfur Radioisotopes; D014018:Tissue Distribution; D015017:Yolk Sac / Q000378:metabolism
D001798:Blood Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine