Separation of some neutral lipids by normal-phase high-performance liquid chromatography on a cyanopropyl column: ubiquinone, dolichol, and cholesterol levels in sheep liver.
The normal-phase high-performance liquid chromatographic separation of neutral lipids into molecular classes was carried out on a cyanopropyl (CN) column eluted with isopropanol in hexane. Cholesteryl, retinyl, and dolichyl esters, triglycerides and vitamin E, ubiquinone, dolichol, phytol, and cholesterol eluted as separate peaks with 0.05% isopropanol in hexane. Cholesterol, retinol, diglyceride, and monoglyceride eluted as separate peaks with 0.75% isopropanol in hexane. These separations could not be achieved on a silica gel column. The method was used to assay sheep liver ubiquinone, dolichol, and cholesterol levels, that were determined as 77, 108 and 1864 micrograms/g wet wt, respectively.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002784:Cholesterol / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004075:Diglycerides / Q000302:isolation & purification; D004224:Diterpenes / Q000302:isolation & purification*; D004286:Dolichols / Q000302:isolation & purification*; D005989:Glycerides / Q000302:isolation & purification; D008055:Lipids / Q000302:isolation & purification*; D008099:Liver / Q000032:analysis*; D012756:Sheep; D014451:Ubiquinone / Q000302:isolation & purification*; D014801:Vitamin A / Q000302:isolation & purification
D004075:Diglycerides; D004224:Diterpenes; D004286:Dolichols; D005989:Glycerides; D008055:Lipids; D014801:Vitamin A; D014451:Ubiquinone; D002784:Cholesterol