[Repair of the nucleoid of sarcoma 37 cells in vitro or in vivo. The effect of HEPES buffer].
The method of sedimentation was used to study the repair of Sarcoma 37 cell nucleoid after irradiation of cells in vitro or after irradiation of mice with a dose of 9 Gy. It was shown that the process of repair in vitro was completed after 10 min of incubation of cells in the nutrient medium while the repair in vivo ceased 120 min after irradiation. In the presence of HEPES buffer (20 mM) in the medium the kinetics of the nucleoid repair in cells incubated in vitro was the same as that in vivo.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects; D003470:Culture Media; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D004273:DNA, Neoplasm / Q000096:biosynthesis* / Q000528:radiation effects; D006531:HEPES / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D051379:Mice; D010879:Piperazines / Q000494:pharmacology*; D012511:Sarcoma 37 / Q000378:metabolism*; D012513:Sarcoma, Experimental / Q000378:metabolism*
D003470:Culture Media; D004273:DNA, Neoplasm; D010879:Piperazines; D006531:HEPES
Reparatsiia nukleoida sarkomy 37 in vitro ili in vivo. Vliianie HEPES-bufera.