Abnormal limb regeneration in adult newts exposed to the fungicide Maneb 80. A histological study.
The effects of the fungicide Maneb 80 (manganese ethylenebisdithiocarbamate, 80% active ingredient) on the regenerating limb of the adult crested newt, Triturus cristatus carnifex, was studied. Female newts were exposed percutaneously to 5 ppm Maneb 80. One group of control newts was exposed to the inert ingredients of Maneb 80 (sodium lignin sulfonate and n-butylnaphthalene sulfonate), and another control group was kept in tap water. The limbs were examined histologically at weekly intervals throughout the regeneration period and at the end of the experiment (10-12 wk postamputation). The regenerating limbs of all the animals exposed to Maneb 80 showed growth retardation and skeletal abnormalities. Histological examination provided evidence that vascular disturbances are important for the genesis of the developmental abnormalities induced by Maneb 80. The inert ingredients had a promoting effect on limb growth and had no teratogenic effects under our experimental conditions. There were no histological differences between the two control groups.
D000014:Abnormalities, Drug-Induced; D000818:Animals; D001808:Blood Vessels / Q000187:drug effects; D005121:Extremities / Q000098:blood supply / Q000473:pathology / Q000502:physiology*; D008344:Maneb / Q000633:toxicity*; D012038:Regeneration / Q000187:drug effects*; D012455:Salamandridae / Q000502:physiology; D013859:Thiocarbamates / Q000633:toxicity*
D013859:Thiocarbamates; D008344:Maneb