Preparation of [14C]uridine 5'-diphosphate and [14C]guanosine 5'-diphosphate.
Procedures have been developed for the routine enzymatic synthesis of [14C]UDP and [14C]GDP from commercially available enzymes and [14C]UMP and [14C]GMP. Using high pressure liquid chromatography, the products are recovered in high yield (60-80%) and with high purity. The [14C]UDP and [14C]GDP are utilized as substrates for ribonucleotide reductase.
D000818:Animals; D002250:Carbon Radioisotopes; D002417:Cattle; D006150:Guanine Nucleotides / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods*; D012262:Ribonucleoside Diphosphate Reductase / Q000378:metabolism; D014500:Uracil Nucleotides / Q000378:metabolism; D014530:Uridine Diphosphate / Q000378:metabolism
D002250:Carbon Radioisotopes; D006150:Guanine Nucleotides; D014500:Uracil Nucleotides; D006153:Guanosine Diphosphate; D014530:Uridine Diphosphate; D012262:Ribonucleoside Diphosphate Reductase