On the application of cultured neuroblastoma cells in chemical toxicity screening.
The acute toxic action of a number of common chemicals was tested by their ability to cause detachment of cultured mouse neuroblastoma C1300 cells. A TD25 value was obtained by graphic estimation of the concentration needed to cause 25% of the total cell number to detach. These TD25 values were compared with LD50 values obtained from the literature, and they were found to correlate with a coefficient of 0.86. For six of the tested substances-diuron, butylated hydroxytoluene, benzidine, cyclophosphamide, Na2SeO3, and KCN-a very poor correlation was obtained. These diverging results could be ascribed to deficiencies in the neuroblastoma cell detachment test and emphasize the necessity for combined in vitro test procedures.
D000818:Animals; D002478:Cells, Cultured; D007928:Lethal Dose 50; D008722:Methods; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology*; D014116:Toxicology