Quantitation of platelet membrane glycoproteins in Glanzmann's thrombasthenia and the Bernard-Soulier syndrome by electroimmunoassay.
A rapid and precise electroimmunoassay for quantitation of glycoprotein Ib and IIb-IIIa in platelet lysates is described. Using this method, platelets from two patients with Type II thrombasthenia contained 8% and 12% of normal levels of IIb-IIIa, whereas IIb-IIIa was undetectable in platelets of one Type I thrombasthenic patient. The level of Ib in two patients with Bernard-Soulier syndrome was essentially nil. The increased precision of this assay, when compared to CIE, allows for estimations of glycoproteins levels varying by no more than a few percent.
D000911:Antibodies, Monoclonal; D001779:Blood Coagulation Factors / Q000032:analysis; D001792:Blood Platelets / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D007122:Immunoelectrophoresis; D008565:Membrane Proteins / Q000097:blood*; D013921:Thrombocytopenia / Q000097:blood*
D000911:Antibodies, Monoclonal; D001779:Blood Coagulation Factors; D006023:Glycoproteins; D008565:Membrane Proteins