[Induction of sensitivity of a fibroblast culture to hypophyseal somatotropin by a thermostable blood serum factor].
A study was made of the effects of highly purified preparations of human and bovine pituitary somatotropin on DNA biosynthesis in fibroblast cultures from adults' skin. The intensity of DNA biosynthesis was evaluated from 3H-thymidine incorporation into the cells. It was established that both somatotropin preparations are capable of stimulating DNA synthesis by fibroblasts. However, simultaneous presence in the medium of the thermo- and acid-resistant fractions of rat blood serum is required for the stimulating effect of the hormone to manifest. It was found that the activity of blood serum factor inducing fibroblast sensitivity to somatotropin depends on the pituitary and rises after hypophysectomy.
D000328:Adult; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D007016:Hypophysectomy; D008297:Male; D010902:Pituitary Gland / Q000502:physiology*; D051381:Rats; D012867:Skin / Q000187:drug effects* / Q000378:metabolism
D013006:Growth Hormone; D004247:DNA
Induktsiia chuvstvitel'nosti kul'tury fibroblastov k gipofizarnomu somatotropinu termostabil'nym faktorom syvorotki krovi.