Transport of alpha-aminoisobutyrate into Trypanosoma brucei brucei.
The uptake of alpha-aminoisobutyrate (AIB) by washed cell suspensions of bloodstream forms of Trypanosoma brucei brucei has been shown to be an energy-dependent process. No metabolism of AIB was detected under conditions leading to a 100-fold accumulation of AIB within the organism. Kinetic studies revealed that AIB uptake involved two components; that operating at low substrate concentrations had an apparent Km of 4.6 mM. Experiments with ionophores such as gramicidin and carbonylcyanide m-chlorophenylhydrazone were consistent with the AIB uptake system operating as a H+-symporter responding to the electrochemical gradient of H+, the major component of which was the membrane potential.
D000621:Aminoisobutyric Acids / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002412:Cations / Q000494:pharmacology; D004563:Electrochemistry; D004734:Energy Metabolism; D007476:Ionophores / Q000494:pharmacology; D007700:Kinetics; D014346:Trypanosoma brucei brucei / Q000187:drug effects / Q000378:metabolism*
D000621:Aminoisobutyric Acids; D002412:Cations; D007476:Ionophores; C100049:2-aminoisobutyric acid