title: Selective binding of human cumulus cell-secreted glycoproteins to human spermatozoa during capacitation in vitro.
abstract: The results of this study demonstrate that glycoproteins manufactured by human cumulus cells can be detected bound to human spermatozoa incubated in capacitational medium containing the labeled cumulus-cell secretions. Cumulus-cell-secreted glycoproteins were labeled with a mixture of 3H-methionine and 3H-tryptophan or with 3H-fucose, and the binding of the labeled compounds to spermatozoa was evaluated by autoradiography. The binding was highly selective, involving only approximately 1% of the samples of spermatozoa used. The results suggest that the binding of cumulus-cell-secreted glycoproteins to spermatozoa may represent a final and highly selective step in human sperm capacitation.
mesh_terms: D000328:Adult; D001345:Autoradiography; D001665:Binding Sites; D003470:Culture Media; D005260:Female; D005643:Fucose / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D008715:Methionine / Q000378:metabolism; D010063:Ovum / Q000378:metabolism*; D013075:Sperm Capacitation; D013094:Spermatozoa / Q000378:metabolism* / Q000502:physiology; D014316:Tritium; D014364:Tryptophan / Q000378:metabolism
chemical_list: D003470:Culture Media; D006023:Glycoproteins; D014316:Tritium; D005643:Fucose; D014364:Tryptophan; D008715:Methionine