title: Membrane solubility of chlorpromazine. Hygroscopic desorption and centrifugation methods yield comparable results.
abstract: Binding of the positively charged drug chlorpromazine to artificial and erythrocyte bilayer membranes was investigated by the filtration method called hygroscopic desorption [Conrad & Singer (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 5202-5206] and by the conventional centrifugation method. Only minor differences in the partition coefficients were observed using the two methods. Our finding is not consistent with the observation of Conrad & Singer that amphipaths are completely excluded from biological membranes. However, the partition coefficient is dependent on membrane composition, which means dependent on the physical properties of a membrane.
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D002498:Centrifugation; D002746:Chlorpromazine / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008051:Lipid Bilayers / Q000378:metabolism; D012995:Solubility
chemical_list: D008051:Lipid Bilayers; D002746:Chlorpromazine