title: Ganglioside pattern of normal human brain, from samples obtained at surgery. A study especially referred to alkali labile species.
abstract: The ganglioside pattern from normal human temporal cortex and cerebellum has been studied in fresh specimens obtained at surgery. The analyses have been performed by two-dimensional thin layer chromatography with an intermediate ammonia treatment which is a methodology particularly suitable for resolving alkali labile gangliosides. Alkali labile gangliosides were detected in all the analyzed specimens and their content contributed to 23% and 11% of total lipid bound sialic acid, in temporal cortex and cerebellum, respectively.
mesh_terms: D000368:Aged; D001923:Brain Chemistry; D002531:Cerebellum / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008875:Middle Aged; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000032:analysis; D013702:Temporal Lobe / Q000032:analysis
chemical_list: D005732:Gangliosides; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid