Effects of phosphate anions and some divalent metal cations on phosphoenolpyruvate carboxykinase. Comparison of liver and kidney enzyme.
The effect of calcium and phosphate anions on rat kidney cytosol phosphoenolpyruvate carboxykinase activity was evaluated using enzyme preparations obtained by two purification procedures. The enzyme activity was not significantly affected by calcium ions at physiological concentration. Phosphate inhibited the enzyme in the presence of Fe2+; the inhibition was overcome by Mn2+. Kidney and liver phosphoenolpyruvate carboxykinases show some qualitative differences in their response to Fe2+ and phosphate.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent; D003600:Cytosol / Q000201:enzymology; D007501:Iron / Q000494:pharmacology; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008345:Manganese / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology*; D010710:Phosphates / Q000494:pharmacology*; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D002413:Cations, Divalent; D008670:Metals; D010710:Phosphates; D008345:Manganese; D007501:Iron; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D002118:Calcium