The cellular substrate: a very important requirement for baculovirus in vitro replication.
We established more than 200 primary cell lines of Cydia pomonella (coding moth). 81 of them were selected and screened for replication of two baculoviruses (from two different subgroups): the Choristoneura murinana NPV and the Cydia pomonella GV. Although all these cell lines had been derived from the same insect species, they varied largely in their response to challenge with the NPV. Most of them showed CPE or produced different amounts of polyhedra. Interestingly, we also found a few cell lines that were permissive for GV replication. To our knowledge this is the first time that GV replication in cell lines has been obtained. Our results show that cell line properties are most important for baculovirus in vitro replication.
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D004261:DNA Replication; D004625:Embryo, Nonmammalian; D006458:Hemolymph / Q000382:microbiology; D007304:Insect Viruses / Q000235:genetics* / Q000276:immunology; D007814:Larva; D009036:Moths; D014779:Virus Replication
D000911:Antibodies, Monoclonal