Peptide antibiotic K-582 production in relation to amino acid metabolism in Metarrhizium anisopliae.
Formation of the basic antibiotic, K-582 was stimulated by supplying Metarrhizium anisopliae U-47 with several amino acids present in its structure. The addition of L-arginine to the basal medium resulted in the almost exclusive formation of K-582 B, while L-lysine increased K-582 A formation. Some carbon sources were observed to have effects similar to those obtained with the above mentioned amino acids. Furthermore, when L-arginine was added in excess to the basal medium, free gamma-hydroxyarginine, which is a major constituent of the antibiotic, accumulated extra- and intra-cellularly. Free gamma-hydroxyarginine isolated from the culture broth of this microorganism was the threo-L-isomer. K-582 formation was repressed by glycerol, which exerted catabolite repression of gamma-hydroxyarginine synthesis.
D000596:Amino Acids / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D023181:Antimicrobial Cationic Peptides; D001120:Arginine / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000494:pharmacology; D002244:Carbon / Q000378:metabolism; D005990:Glycerol / Q000494:pharmacology; D003904:Mitosporic Fungi / Q000378:metabolism*; D010452:Peptide Biosynthesis; D010455:Peptides
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D010455:Peptides; C007985:N(5)-hydroxy-L-arginine; C026530:myroridin K; D002244:Carbon; D001120:Arginine; D005990:Glycerol