Determination of ethylenediamineplatinum(II) malonate in infusion fluids, human plasma and urine by high-performance liquid chromatography.
A selective and convenient high-performance liquid chromatographic assay was developed for ethylenediamineplatinum(II) malonate (JM-40) in plasma ultrafiltrate and urine. A mu Porasil silica column (30 cm) was used with acetonitrile-water (90:10, v/v) as the mobile phase and the elution of compounds was monitored by ultraviolet absorbance at 214 nm. A linear dynamic range of at least three decades (1-1000 micrograms/ml) was achieved. The detection limit in plasma ultrafiltrate was 0.35 micrograms/ml. The stability of JM-40 was determined in 0.9% sodium chloride, 5% glucose, plasma ultrafiltrate and urine. More stable drug solutions were obtained with 5% glucose than with 0.9% sodium chloride. JM-40 was also determined in plasma ultrafiltrate and urine samples of one patient receiving short-term infusions of the drug. In plasma ultrafiltrate unmetabolized JM-40 was detected during the first 5 h after administration and had a half-life of 21.3 +/- 1.6 min. The parent drug was excreted in the urine in rapidly decreasing amounts. Eighteen per cent of the dose was recovered as unmetabolized drug during the first 6 h.
D000970:Antineoplastic Agents / Q000032:analysis* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D006801:Humans; D007263:Infusions, Parenteral; D007700:Kinetics; D009944:Organoplatinum Compounds / Q000032:analysis* / Q000378:metabolism; D010984:Platinum / Q000097:blood; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013997:Time Factors
D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D012996:Solutions; C038327:ethylenediamine platinum(II) malonate; D010984:Platinum