The determination of trimazosin and its metabolite CP23445 in whole blood by high performance liquid chromatography using fluorescence detection.
A sensitive and specific assay for trimazosin and its metabolite CP23445 is described. The method employs reverse-phase high performance liquid chromatography (HPLC) with fluorescence detection. The limit of detection of the assay is 1 ng/ml whole blood for trimazosin and 0.5 ng/ml for CP23445. The coefficient of variation for trimazosin and CP23445 over a typical calibration size is 5.4% and 4.9%, respectively. Various drugs that may be coadministered with trimazosin are shown not to interfere in the assay. The method has been extensively employed to study the pharmacokinetics of trimazosin and CP23445, both in man and in animals.
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008657:Metabolic Clearance Rate; D010879:Piperazines / Q000097:blood* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D010879:Piperazines; C005185:trimazosin; C040290:1-hydroxytrimazosin