[Viscosimetric analysis of the dynamics of alkaline lysis of mammalian cell nuclei as a method of studying radiation damage to DNA structures].
The method is proposed for the analysis of DNA viscosity during alkaline lysis of rat thymocyte nuclei which permits to reveal early changes in the DNA structure at doses of 0.5 to 1 Gy. Doses from 0.5 to 30 Gy were chosen for the experiment. The changes in the lysis dynamics, the decrease in the intrinsic viscosity maximum [eta], and the increase in the alkaline lysis rate were shown to be a function of radiation dose.
D000818:Animals; D002467:Cell Nucleus / Q000528:radiation effects*; D003037:Cobalt Radioisotopes; D004247:DNA / Q000528:radiation effects*; D003902:Detergents; D005720:Gamma Rays; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000166:cytology; D013997:Time Factors; D014783:Viscosity
D003037:Cobalt Radioisotopes; D003902:Detergents; D004247:DNA
Viskozimetricheskiĭ analiz dinamiki shchelochnogo lizirovaniia iader kletok mlekopitaiushchikh kak metod izucheniia radiatsionnogo porazheniia struktury DNK.