Liquid-liquid extraction of membranes from calf brain using conventional and centrifugal counter-current distribution techniques.
Neural membranes isolated from calf brain have been partitioned in aqueous two-phase systems containing dextran and polyethyleneglycol. When the partition was repeated several times, using counter-current distribution technique, the distribution of the membranes between the upper phase and the interface changed in a non-ideal manner and in favour of the interface. By using a centrifugal counter-current distribution device the time for the experiment could be reduced by a factor of 7-8 and the distribution was similar to what could be expected for ideally behaving membranes. The time-dependent change of the membranes is discussed in terms of aggregation and lateral membrane perturbations. Despite this effect a certain fractionation has been achieved as deduced from analysis of cholesterol content, opiate receptor activity and acetylcholinesterase activity along the counter-current distribution row of fractions. Compared to the starting material these activities were enriched some two-fold in certain fractions.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002498:Centrifugation; D003377:Countercurrent Distribution / Q000295:instrumentation; D008566:Membranes / Q000032:analysis; D009474:Neurons / Q000032:analysis; D011189:Potassium Chloride / Q000032:analysis; D013570:Synaptic Membranes / Q000032:analysis
D011189:Potassium Chloride