Molecular cloning and nucleotide sequence of a variant wheat histone H4 gene.
To determine whether there is structural variation among histone H4 genes in wheat, one (TH091) of the H4 genes that had been cloned from a wheat genomic DNA library was sequenced and compared with another H4 gene (TH011) which we had described previously [Tabata et al., Nucl. Acids Res. 11 (1983) 5865-5865]. Nucleotide sequence analysis revealed that there are 17 nucleotide replacements in the protein-coding region of two H4 genes, causing only one amino acid substitution: a glycine at position 4 (from the N terminus) in TH011 was replaced by an aspartic acid in TH091. S1 mapping, using total nuclear RNA from germinated seeds, indicated that the H4 gene was transcribed in vivo.
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000032:analysis; D014644:Genetic Variation; D006657:Histones / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D014158:Transcription, Genetic; D014908:Triticum / Q000235:genetics*
D004274:DNA, Recombinant; D006657:Histones; D010940:Plant Proteins