Complement activation according to the alternate pathway by glass and plastic surfaces and its role in neutrophil adhesion.
C3-deposition, generated by complement activation according to the alternate pathway, was detected on borosilicate glass slides and polystyrene Petri dishes. The C3-depositions grew peripherally until the entire surface was covered. The deposits were also visualized with scanning electron microscopy and could not be washed away with low-pH (3.5) or high-pH (9.6) buffers. No consumption of complement function was detected indicating a phenomenon restricted to the glass and plastic surfaces. The C3-deposits could mediate an adherence of human neutrophils.
D002448:Cell Adhesion; D003167:Complement Activation; D003176:Complement C3 / Q000276:immunology; D003170:Complement Pathway, Alternative; D005898:Glass; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000276:immunology*; D010969:Plastics; D013499:Surface Properties
D003176:Complement C3; D010969:Plastics