Synthesis of three N-nitroso dipeptides N-terminal in proline and a method for their determination in food.
The terminal N-nitroso derivatives of L-prolyl-L-alanine, L-prolylglycine and L-prolyl-4-hydroxy-L-proline have been synthesized and characterized. A procedure suitable for their analysis in biological samples has been developed and is based on the preparation of an aqueous extract, extraction into an organic solvent, separation by high-performance liquid chromatography and detection by a chemiluminescence method. The average recovery in all cases was above 69% at the level of 200 micrograms/kg using cured meat as a food matrix; the detection limit was 10 micrograms/kg.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004151:Dipeptides / Q000032:analysis* / Q000138:chemical synthesis; D005504:Food Analysis; D008163:Luminescent Measurements; D008460:Meat / Q000032:analysis; D009602:Nitrosamines / Q000032:analysis* / Q000138:chemical synthesis; D011392:Proline / Q000032:analysis*
D004151:Dipeptides; D009602:Nitrosamines; C046501:N-nitroso-prolylglycine; C046502:N-nitroso-prolyl-4-hydroxyproline; C046500:N-nitroso-prolylalanine; D011392:Proline