[Kinetics of the steroidogenic response of the testis to stimulation by hCG. V. Blockade of 17-20 lyase induced by hCG is an age-dependent phenomenon inducible by pre-treatment with hCG].
The series of events, evidenced in animals after a single injection of human chorionic gonadotrophin (hCG): down-regulation of LH/hCG membrane receptors, uncoupling between receptors and the adenylate cyclase, also includes a blockade of testicular steroidogenesis beyond cyclic-AMP formation, including an inhibition of the 17 alpha-hydroxylase-17, 20-desmolase enzymatic complex. This complex phenomenon, named hCG-induced testicular desensitization also occurs in adult men. Since it is not known if and when these effects are initiated during sexual maturation, we have investigated the kinetics of responses of plasma testosterone (T), its two immediate precursors, delta 4-androstenedione (delta 4) and 17 alpha-hydroxprogesterone (OHP), and 17 beta-estradiol (E2) for a week after a single injection of hCG given at the same dosage (100 IU/kg body weight) in subjects not yet exposed to adult levels of endogenous LH, ie prepubertal boys and untreated hypogonadotrophic hypogonadic (HH) adult men. The HH subjects have been restudied after 3 months of a weekly injection of the hCG at the same dosage. In immature individuals, the effect of hCG on testicular steroidogenisis was strikingly different from that observed previously in adults: in the former, whether prepubertal boys or untreated HH adults, a single hCG injection induced a progressive and substantial rise in plasma T, maximal at 96-120 h, a modest and late rise in E2, but no significant change in delta 4 or OHP. In contrast, in adult men there is a dissociation between the responses of plasma T and delta 4 (maximal at about 72 h) to hCG and those of OHP and E2 which peak at about 24 h. After 3 months of hCG-treatment the adult pattern was induced in HH patients: early and significant rise in OHP and E2. This suggests that a pre-exposure to LH/hCG is necessary for hCG-induced testicular desensitization, at least for its enzymatic expression. A stimulatory effect of hCG on a testicular aromatase and an inhibitory effect on the 17, 20-desmolase are observed concomitantly in relation to age or to previous gonadotropin environment. It still remains uncertain to conclude that the former effect is directly responsible for the latter.
D000328:Adult; D000367:Age Factors; D000446:Aldehyde-Lyases / Q000037:antagonists & inhibitors*; D000818:Animals; D002648:Child; D006063:Chorionic Gonadotropin / Q000627:therapeutic use*; D006801:Humans; D007006:Hypogonadism / Q000188:drug therapy; D007700:Kinetics; D008297:Male; D051379:Mice; D051381:Rats; D013045:Species Specificity; D013254:Steroid 17-alpha-Hydroxylase; D013256:Steroids / Q000096:biosynthesis*; D013552:Swine; D013577:Syndrome; D013737:Testis / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D013256:Steroids; D013254:Steroid 17-alpha-Hydroxylase; D000446:Aldehyde-Lyases
Cinétique de la réponse stéroïdogénique testiculaire à la stimulation par l'hCG. V. Le blocage de la 17-20 lyase induit par l'hCG est un phénomène âge-dépendant, inductible par un pré-traitement à l'hCG.