title: [Multiple forms of malate dehydrogenase (EC 1.1.1.37) in Crithidia asclepii and C. oncopelti (Mastigophora, Trypanosomatidae)].
abstract: Isozyme analysis of mMDH in Crithidia asclepii and C. oncopelti by means of electrophoresis and densimetry method suggests the diploidness and genetic exchange in these organisms. Synthesis of mMDH in C. asclepii is checked by duplicated polymorphic loci MDH-1 and MDH-2, each with two alleles. In C. oncopelti two "daughter" loci are functioning too, polymorphic MDH-1 with 3 alleles and monomorphic MDG-2. Resemblance and differences in the isozyme spectra of mMDH can be used for taxonomic purposes.
mesh_terms: D000483:Alleles; D000818:Animals; D003421:Crithidia / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D003720:Densitometry; D004171:Diploidy; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000032:analysis* / Q000235:genetics; D008291:Malate Dehydrogenase / Q000032:analysis* / Q000235:genetics; D013053:Spectrophotometry
chemical_list: D007527:Isoenzymes; D008291:Malate Dehydrogenase
vernacular_title: Mnozhestvennye formy malatdegidrogenazy (K.F.1.1.1.37) u Crithidia asclepii i C. oncopelti (Mastigophora, Trypanosomatidae).