Fluorescence labelling of pyrimidine nucleobases and their related compounds for high-performance liquid chromatography.
Fluorescence derivatization of pyrimidine nucleobases and nucleosides with 4-bromomethyl-7-methoxycoumarin(Br-Mmc) was studied. In dimethylsulfoxide(DMSO) containing powdered potassium carbonate, the reaction completed within 5 min at room temperature. The excess Br-Mmc was treated with p-nitrobenzoic acid(NBA) which gave a non-fluorescent compound. For the determination of serum sample, diethylaminoethyl sepharose(DEAE) column was used in a cleanup procedure.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003374:Coumarins; D005456:Fluorescent Dyes; D006801:Humans; D007202:Indicators and Reagents; D011743:Pyrimidines / Q000097:blood / Q000138:chemical synthesis* / Q000302:isolation & purification; D013329:Structure-Activity Relationship
D003374:Coumarins; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D011743:Pyrimidines; C036126:4-bromomethyl-7-acetoxycoumarin