Dissociation between cytostatic and cytolytic activity of human monocytes towards K-562 cells.
The cytolytic and cytostatic activities of human monocytes towards K-562 cells were compared by determining release of 3H-thymidine and DNA synthesis rate (i.e. thymidine incorporation), respectively. The inter-individual variations were substantial, with significant correlation between cytostasis and cytolysis in the entire experimental material (26 blood donors), but not in monocytes kept in vitro for 90 min or 1 day before the start of assay. A significant level of correlation between cytolysis and cytostasis was observed in monocytes kept in vitro for 4 days before assay (coefficient of correlation = 0.7). The low degree of correlation at each test-time-point prohibits the prediction of cytostatic capability of human monocytes in individual experiments from determination of monocyte-mediated target cell lysis.
D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007938:Leukemia / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D013936:Thymidine