[Quantitative determination of total mitochondrial protein with Coomassie].
The mechanism of Coomassie interaction with proteins was being studied. Under certain conditions the range of detectible polypeptides can be wider. For protein analysis in mitochondria we have suggested a new component ratio for the Bradford reagent. A correction for mitochondrial protein was determined, which should be made on using bovine serum albumin as a standard.
D000818:Animals; D004347:Drug Interactions; D007202:Indicators and Reagents; D008722:Methods; D008930:Mitochondria, Liver / Q000032:analysis*; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D051381:Rats; D012394:Rosaniline Dyes / Q000494:pharmacology*
D007202:Indicators and Reagents; D011506:Proteins; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue
Kolichestvennoe opredelenie obshchego belka mitokhondriĭ s kumassi.