Purification of rat liver microsomal cytochrome P-450 by chromatography on immobilized adamantane with a new method of detergent removal.
A method for the isolation of liver microsomal cytochrome P-450 from phenobarbital-treated rats is described which is based on chromatography on 1-adamantane-carbonyl-aminohexyl-Sepharose 4B, subsequent ion exchange chromatography on DEAE-cellulose and detergent removal by adsorption on silica gel G. The method gives preparations with high electrophoretic homogeneity which correspond to a major phenobarbital-inducible form of rat liver microsomal cytochrome P-450. The advantages of the procedure described are high yields, stability of chromatographic material, effective and simple method of detergent removal.
D000218:Adamantane; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification*; D008297:Male; D008862:Microsomes, Liver / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D003577:Cytochrome P-450 Enzyme System; D000218:Adamantane