Harmaline induced tremor. III. A combined simple units, horseradish peroxidase, and 2-deoxyglucose study of the olivocerebellar system in the rat.
Purkinje cells were recorded extracellularly and mapped in the cerebellar cortex of the rat under tremogenic doses of harmaline. Four different types of responses were encountered, of which two were considered as being responsible for the harmaline tremor. The latter had a regular firing pattern of complex spikes at 5 to 10 Hz and were mostly found in the vermis. Their number decreased in the more lateral region of the cerebellar cortex until they eventually disappeared. Horseradish peroxidase was injected into all the areas of the cerebellar cortex containing Purkinje cells with harmaline-induced activity. Labeled neurons were in all cases traced to the medial accessory olive. The metabolic activity of the inferior olive under harmaline was measured with 2-deoxyglucose. Increased labeling was only found in the medial accessory olive. Such an increase was demonstrated as being due to a direct effect of the drug on the inferior olivary neurons, indicating that the medial accessory olive is responsible for the harmaline tremor in the rat. Our results point out that, in the rat, there is an inverse relationship between serotoninergic innervation of a region in the inferior olivary nucleus and that with harmaline sensitivity, therefore a serotoninergic mechanism hypothesis for the harmaline tremor needs further investigation.
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D001931:Brain Mapping; D005947:Glucose / Q000378:metabolism; D006246:Harmaline / Q000494:pharmacology*; D009434:Neural Pathways / Q000503:physiopathology; D009847:Olivary Nucleus / Q000187:drug effects* / Q000503:physiopathology; D011689:Purkinje Cells / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D014202:Tremor / Q000139:chemically induced* / Q000503:physiopathology
D000470:Alkaloids; D006246:Harmaline; D005947:Glucose