Preparation of anti-Lea and anti-Rh0 (D) sera by immunization with blood group substance trapped in autologous red cell ghost.
Lea substance or immune complex which was prepared with anti-D and D-active Band 3 was trapped in autologous red cell ghost. The trapped immunogens were administered intravenously to rabbits or guinea pigs. Rabbits immunized with Lea substance loading ghost produced antisera of relatively low titers, but they contained specific incomplete anti-Lea antibody (titer 1:128) after absorption procedure, which were higher than that of antisera prepared by the ordinary method. Guinea pig antiserum to the immune complex contained specific incomplete anti-D (titer 1:128). Immunoglobulin class analyses revealed that the anti-Lea consisted mainly of IgM and the anti-D of IgG. It was considered that intravenous injection of the immunogen trapped in ghost is useful for preparing hemagglutinin of sufficient titers.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology; D004910:Erythrocyte Membrane / Q000276:immunology; D006168:Guinea Pigs; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007518:Isoantibodies / Q000096:biosynthesis*; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D011817:Rabbits
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007518:Isoantibodies; D007983:Lewis Blood Group Antigens; C033242:RH-antibodies