Quantitative evaluation of guinea pig spermatogenesis: Epon versus paraffin embedding.
A quantitative evaluation of spermatogenesis in the guinea pig was made at the light microscope level following improved methods of fixation and plastic embedding. The pattern of germ cell differentiation was studied by counting the average number of germ cell nuclei per Sertoli cell nucleus during the seminiferous epithelial cycle. Appreciable cell loss occurred during spermatogenesis. As a result, only 20.8% of the theoretically possible number of spermatozoa were produced from each spermatogonium. A slightly better yield of germ cells was obtained from Epon sections in comparison to paraffin sections. Epon sections can be effectively used for quantitative analyses of various cell populations in small biopsy specimens of testis tissue.
D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D004847:Epithelial Cells; D004853:Epoxy Resins; D006168:Guinea Pigs; D006651:Histocytochemistry / Q000379:methods*; D008297:Male; D010232:Paraffin; D012671:Seminiferous Tubules / Q000166:cytology; D012708:Sertoli Cells / Q000166:cytology; D013091:Spermatogenesis; D013093:Spermatogonia / Q000166:cytology
D004853:Epoxy Resins; C004875:EPON; D010232:Paraffin