The essential cationic charge of phospholipid polar head in the reactivation of D-beta-hydroxybutyrate apodehydrogenase revealed by cationic surfactants.
Attempts to reactivate purified D-beta-hydroxybutyrate apodehydrogenase, a lecithin-requiring enzyme, have been carried out using neutral, anionic, cationic and zwitterionic surfactants. Cationic and zwitterionic compounds exclusively are able to partially replace phosphatidylcholine, the reactivating phospholipid. The extent of reactivation depends on the steric hindrance of the polar head and on the hydrophobic tail length. A molecule bearing a positive charge and an aliphatic chain is the sole structure absolutely required for activity. However the presence of a negative charge is important for enzyme binding to amphiphilic structures and for the efficiency of reactivation.
D000818:Animals; D000838:Anions; D001051:Apoenzymes / Q000378:metabolism*; D001059:Apoproteins / Q000378:metabolism*; D002412:Cations; D004789:Enzyme Activation; D004793:Enzyme Reactivators / Q000494:pharmacology*; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism*; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D010713:Phosphatidylcholines / Q000494:pharmacology; D010743:Phospholipids / Q000494:pharmacology*; D051381:Rats; D013329:Structure-Activity Relationship; D013501:Surface-Active Agents / Q000494:pharmacology*
D000838:Anions; D001051:Apoenzymes; D001059:Apoproteins; D002412:Cations; D004793:Enzyme Reactivators; D010713:Phosphatidylcholines; D010743:Phospholipids; D013501:Surface-Active Agents; D009243:NAD; D006884:Hydroxybutyrate Dehydrogenase