Chromatographic resolution of the rod pigment from the four cone pigments of the chicken retina.
Chicken rod and cone pigments are chromatographically resolved using a combination of stepwise elution from a concanavalin A affinity column and a secondary ion exchange purification. The rod pigment rhodopsin and the cone pigment iodopsin are purified to homogeneity. There is a partial separation of three other cone pigments: chicken green, chicken blue, and chicken violet, making clear that this is at least a tetrachromatic cone system.
D000818:Animals; D002645:Chickens; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D010786:Photoreceptor Cells / Q000032:analysis*; D012168:Retinal Pigments / Q000032:analysis*; D012243:Rhodopsin / Q000032:analysis; D017299:Rod Opsins; D013053:Spectrophotometry
D012168:Retinal Pigments; D017299:Rod Opsins; C034941:iodopsin; D012243:Rhodopsin