Heterogeneity of collagen isolated from methylcholanthrene-induced sarcoma.
Rat fibrosarcoma induced by subcutaneous injection of methylcholanthrene was found to contain at least three different types of collagen. Two of them were identified as type I and type III collagens, the third (fraction B) seems to be specific for this tumour. The ratio of type I to type III collagen is lower in fibrosarcoma than in normal rat skin. The number of hydroxyproline residues in alpha 1 (I), alpha 2 (I) and alpha 1 (III) chains of tumour collagen appears to be higher than in the corresponding chains of rat skin collagen. Fraction B is composed of three identical alpha chains connected with disulphide bonds. It contains a relatively low amount of glycine: 234 molecules per 1000 residues. The amount of hydroxyproline and cysteine is similar to that found in the type III collagen.
D000478:Alkylation; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003094:Collagen / Q000032:analysis*; D004220:Disulfides / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006601:Hexoses / Q000032:analysis; D006909:Hydroxyproline / Q000378:metabolism; D008748:Methylcholanthrene; D009363:Neoplasm Proteins / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012513:Sarcoma, Experimental / Q000139:chemically induced / Q000378:metabolism*; D012867:Skin / Q000032:analysis
D000596:Amino Acids; D004220:Disulfides; D006601:Hexoses; D009363:Neoplasm Proteins; D008748:Methylcholanthrene; D003094:Collagen; D006909:Hydroxyproline