The mutant vasopressin gene from diabetes insipidus (Brattleboro) rats is transcribed but the message is not efficiently translated.
The vasopressin gene from normal and diabetes insipidus (Brattleboro) rats has been isolated and sequenced. Except for a single deletion of a G residue in region coding for the neurophysin carrier protein the approximately 2300 nucleotides of both genes are identical. Blot analysis of hypothalamic RNA as well as transfection and microinjection experiments indicate that the mutant gene is correctly transcribed and spliced, however the resulting mRNA is not efficiently translated.
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D003919:Diabetes Insipidus / Q000235:genetics*; D005796:Genes; D009154:Mutation; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011910:Rats, Brattleboro; D014158:Transcription, Genetic; D014667:Vasopressins / Q000235:genetics*
D012333:RNA, Messenger; D014667:Vasopressins; D004247:DNA