title: Effects of fibrinogen degradation products on glomerular mesangial cells in culture.
abstract: We investigated effects of fibrin, fibrinogen, and fibrinogen degradation products (FDPs) on human glomerular mesangial cells in culture, using the methods of cell count, 3[H] thymidine uptake and 51Cr release. Incorporation of 3[H] thymidine by the cells was much the same with various concentrations of fibrin and similar to findings in the control without fibrin. Fibrinogen, FDP-D, -E, low molecular weight FDP (LMWFDP) fractions 1 and 3 had no promoting effects on mesangial cell proliferation. The LMWFDP fraction 2 showed a suppressive effect on the proliferation of cultured cells and increase of 51Cr release from the cells. The cytotoxic effect of this fraction was dose-dependent. These results suggest that while fibrin deposition in the renal glomeruli does not relate with mesangial hypercellularity, this deposition plays a role in mesangiolysis, through the production of FDPs.
mesh_terms: D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002860:Chromium Radioisotopes; D003470:Culture Media; D005337:Fibrin / Q000494:pharmacology; D005338:Fibrin Fibrinogen Degradation Products / Q000494:pharmacology*; D005340:Fibrinogen / Q000494:pharmacology; D005920:Glomerular Mesangium / Q000166:cytology*; D006801:Humans; D014568:Urokinase-Type Plasminogen Activator / Q000494:pharmacology
chemical_list: D002860:Chromium Radioisotopes; D003470:Culture Media; D005338:Fibrin Fibrinogen Degradation Products; D005337:Fibrin; D005340:Fibrinogen; D014568:Urokinase-Type Plasminogen Activator