[Residual structures revealed after the action of staphylococcal nuclease on decondensed metaphase chromosomes].
The isolated Chinese hamster metaphase chromosomes were decondensed in 0.6 M NaCl solution and digested by staphylococcal nuclease on electron microscope grids. The residual structures as seen in electron microscope as isolated aggregates scattered over the entire area occupied by the chromosome. Prior to digestion, the material in the chromosome did not form presumably an integral protein structure similar to a scaffold.
D000818:Animals; D002478:Cells, Cultured; D002875:Chromosomes / Q000187:drug effects* / Q000648:ultrastructure; D006224:Cricetinae; D003412:Cricetulus; D006657:Histones / Q000302:isolation & purification; D008677:Metaphase / Q000187:drug effects*; D008836:Micrococcal Nuclease / Q000494:pharmacology*; D008854:Microscopy, Electron
D006657:Histones; D008836:Micrococcal Nuclease
Ostatochnye struktury, obnaruzhivaemye posle deĭstviia stafilokokkovoĭ nukleazy na dekondensirovannye metafaznye khromosomy.