Absence of correlation between ACh-induced Ca influx and phosphatidic acid labeling in rat uterus.
Rat uterine smooth muscle was preincubated in Ca-depleted modified Locke-Ringer solution to investigate the correlation between the 32Pi incorporation into phosphatidic acid induced by acetylcholine and the contractile response to acetylcholine induced by the addition of CaCl2 (Ca influx). The results showed that in rat uterine smooth muscle under these conditions phosphatidic acid does not act as a Ca ionophore or as a trigger for opening the Ca channel.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D010712:Phosphatidic Acids / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D051381:Rats; D014590:Uterine Contraction / Q000187:drug effects; D014599:Uterus / Q000378:metabolism*
D010712:Phosphatidic Acids; D010761:Phosphorus Radioisotopes; D000109:Acetylcholine; D002118:Calcium