The rate of initiation of alpha- and beta-globin mRNA translation is modulated by 50 kDa, 28-kDa and 24-kDa polypeptide-containing fractions.
The relative rates of initiation of alpha- and beta-globin mRNA translation in a Krebs II ascites cell-free system are differently modulated by a 50-kDa protein and two fractions containing either a 28-kDa or a 24-kDa polypeptide. Each of these fractions stimulated a discrete step that limits initiation of protein synthesis, but other rate-limiting steps take place upstream and/or downstream, resulting in characteristic kinetics of the stimulation of alpha- and beta-globin synthesis. The ascites extracts appear to be deficient in these activities.
D000818:Animals; D002287:Carcinoma, Krebs 2 / Q000378:metabolism; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D005914:Globins / Q000096:biosynthesis* / Q000235:genetics; D007700:Kinetics; D008970:Molecular Weight; D010455:Peptides / Q000502:physiology*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000502:physiology*
D010455:Peptides; D012333:RNA, Messenger; D005914:Globins