[Synthesis and secretion of immunoglobulins in the small intestine of the rat immunized with horseradish peroxidase].
Fifty mature female rats of August strain are immunized with three intraperitoneal injections of horseradish peroxidase (HP) on the 1st, 14th and 28th days of the experiment. On the 42nd day of the experiment a resolution dose of HP is injected through the gastric catheter. The distal Peyer's patch with the adjoining area of the intestine and the proximal part of the jejunum are taken every two days after injecting the resolution dose of the antigen. After fixation in 8% solution of paraformaldehyde and washing, the sections 15 mcm thick are incubated in antigen solution. The complex antigen-antibody is determined by visualization of HP activity using R. C. Graham and M. J. Karnovsky method (1966). The first plasma cells with a positive intracellular reaction in the lamina propria of the small intestine appear on the 4th day after the oral immunization. Immunoglobulins in antibody-synthesizing cells fill the canals of the granular endoplasmic reticulum, as well as the Golgi complex cisterns and the perinuclear space. The anti-HP antibodies are revealed in the interepithelial spaces of the small intestine tunica mucosa and in numerous micropinocytic vesicles of enterocytes. A suggestion is made on a possible diffuse transmission of antibodies via microvilli of enterocytes.
D000818:Animals; D004848:Epithelium / Q000276:immunology; D005260:Female; D006735:Horseradish Peroxidase / Q000276:immunology; D007136:Immunoglobulins / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000276:immunology*; D010581:Peyer's Patches / Q000276:immunology; D010873:Pinocytosis; D010950:Plasma Cells; D051381:Rats
D007136:Immunoglobulins; D006735:Horseradish Peroxidase
Sintez i sekretsiia immunoglobulinov v tonkoĭ kishke krys, immunizirovannykh peroksidazoĭ khrena.