title: Changes in the plasma concentration of immunoreactive arginine vasotocin during oviposition in the domestic fowl.
abstract: The plasma concentrations of immunoreactive arginine vasotocin (AVT) were measured during oviposition and shortly before ovulation of the first egg (Cl) of a clutch. Immunoreactive AVT was determined on bentonite extracts of 0.5 ml plasma samples using the method of Rosenbloom and Fisher (1974). The R-70 antiserum used to measure AVT cross reacted with arginine vasopressin (AVP), however the fowl pituitary does not synthesise AVP. Over a period of 10 to 90 min before oviposition the plasma AVT concentration was about 20 pg/ml; during oviposition it increased four-fold. Measurements made at frequent intervals showed that plasma AVT concentration increased 5 to 6 min before oviposition, reached a peak during oviposition itself and decreased rapidly in the following 5 to 6 min. The surge in plasma AVT occurred on average 48 min before Cl ovulation.
mesh_terms: D000818:Animals; D002645:Chickens / Q000097:blood / Q000502:physiology*; D002850:Chromatography, Gel; D005260:Female; D007457:Iodine Radioisotopes; D010058:Oviposition; D014668:Vasotocin / Q000097:blood*
chemical_list: D007457:Iodine Radioisotopes; D014668:Vasotocin