[Detection of complexes in the rat liver which contain lactate dehydrogenase, using centrifugation in a medium with a dissolved enzyme].
Liver and muscle extracts were fractionated by ultracentrifugation under conditions providing for the maintenance of the lactate dehydrogenase (LDH) containing complexes in the media with LDH. Under these conditions, part of the LDH activity (20-30%) was detected in the fractions corresponding to the particles with Mr 1 000 000-2 000 000. When LDH was substituted for by albumin, no LDH containing complexes were revealed. After preliminary fractionation of liver extracts into heavy and light components, the LDH containing complexes were found only in the light fraction, which points to their specific binding to the enzyme as well as to the presence of such complexes in the intact cell.
D000818:Animals; D005591:Chemical Fractionation; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D014461:Ultracentrifugation
D007770:L-Lactate Dehydrogenase
Obnaruzhenie v pecheni krysy kompleksov, soderzhashchikh laktatdegidrogenazu, metodom tsentrifugirovaniia v srede s rastvorennym fermentom.