Nuclear-cytoplasmic distribution of ions in the hepatocyte.
To determine if nuclear/cytoplasmic (N/C) gradients of ions exist in mammalian hepatocytes, as has been shown to exist in the amphibian oocyte, small pieces of mouse liver were cryofixed for energy dispersive X-ray microanalysis of Na, K and Cl in thin freeze-dried cryosections. Expressing the data on a dry weight basis showed small but significant N/C gradients for Na and K but not for Cl. When the data were expressed on a wet weight basis no significant N/C gradients of Na, K or Cl were revealed. Questions of data interpretation are discussed.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis*; D002713:Chlorine / Q000032:analysis*; D003593:Cytoplasm / Q000032:analysis*; D004577:Electron Probe Microanalysis; D005629:Frozen Sections; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology*; D008297:Male; D051379:Mice; D011188:Potassium / Q000032:analysis*; D012964:Sodium / Q000032:analysis*
D002713:Chlorine; D012964:Sodium; D011188:Potassium