Characterization of Campylobacter jejuni/coli-isolates from human faeces.
Campylobacter jejuni/coli strains were isolated from the faeces of 240 patients suffering from acute enteritis. The following characteristics were investigated: (i) growth at different temperatures, and on different substrates under either microaerophilic conditions or anaerobically, with fumarate or nitrate as terminal electron acceptors; (ii) production of H2S in cysteine-containing broth; (iii) hydrolysis of hippuric acid; (iv) DNase; (v) alkaline phosphatase; (vi) beta-lactamase; (vii) presence of menaquinone; and (viii) reduction of selenite. Based on characteristics (ii)-(v), the strains could be divided in 9 phenotypical groups. Most of the strains represented group 2 (DNase+, H2S+, hippurate hydrolysis+, alk. phosphatase-) (32%), and groups 8 (DNase-, H2S+, hippurate hydrolysis+, alk. phosphatase-) (32%). The other groups were of minor importance. On the other hand, most of the isolates from the United States (Weaver, 1981) fitted well into group 1 (DNase+, H2S+, hippurate hydrolysis+, alk. phosphatase+) which might demonstrate geographical variations among C. jejuni/coli.
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D002168:Campylobacter fetus / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D002374:Catalase / Q000096:biosynthesis; D003851:Deoxyribonucleases / Q000096:biosynthesis; D003967:Diarrhea / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D006626:Hippurates / Q000378:metabolism; D006801:Humans; D006862:Hydrogen Sulfide / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D010089:Oxidoreductases, N-Demethylating / Q000096:biosynthesis; D020887:Selenious Acid; D012643:Selenium / Q000378:metabolism; D013696:Temperature
D006626:Hippurates; D009566:Nitrates; D002374:Catalase; D010089:Oxidoreductases, N-Demethylating; C022726:tetramethylphenylenediamine oxidase; D003851:Deoxyribonucleases; D000469:Alkaline Phosphatase; D020887:Selenious Acid; D012643:Selenium; C030514:hippuric acid; D006862:Hydrogen Sulfide