title: Fluorometric assay of human serum carnosinase activity in normal children, adults and patients with myopathy.
abstract: A sensitive, accurate method has been established for the assay of serum carnosinase by measuring the fluorescence emitted from the L-histidine liberated on treatment with o-phthaldialdehyde. Using this method the serum values for normal adults, infants and children were measured. The mean value was very low in infants of less than 1 year old but increased with age, being almost the same in children aged 6 years or more as in adults. In adult men, the mean activity was 1.85 mumol/mL/h and in adult women it was 2.07 mumol/mL/h. Low activity was observed in patients with muscular dystrophy.
mesh_terms: D000328:Adult; D002648:Child; D002675:Child, Preschool; D004150:Dipeptidases / Q000097:blood*; D005260:Female; D006639:Histidine / Q000097:blood; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007700:Kinetics; D008297:Male; D009135:Muscular Diseases / Q000201:enzymology*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D006639:Histidine; D004150:Dipeptidases; C019673:aminoacyl-histidine dipeptidase