High-performance liquid chromatography with amperometric determination of plasma tyramine.
A method for the measurement of tyramine in human plasma is described. It is based on tetraphenylboron ion-pair extraction and reversed-phase ion-pairing liquid chromatography with amperometric detection. Tyramine can be reliably measured in the range 5-200 ng/ml with an absolute limit of detection of 0.50 ng/ml at a signal-to-noise ratio of 2.0. Correction for variable recovery is made by using a tritiated tyramine internal standard. This assay is suitable for studies on the bioavailability of ingested tyramine and should thus have a role in the development of safer monoamine oxidase inhibitor drugs.
D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013997:Time Factors; D014439:Tyramine / Q000097:blood*
D014439:Tyramine