Acquisition of native structure from reduced Trimeresurus flavoviridis phospholipase A2.
Oxidation of reduced T. flavoviridis phospholipase A2 under suitable conditions resulted in recovery of its active, native structure. The oxidized product was eluted at the same positions as native phospholipase A2 on reserved phase column and DEAE-Toyopearl 650M column chromatographies. The native and regenerated proteins were also identical in mobility on polyacrylamide gel electrophoresis and in circular dichroism spectra. Des-octapeptide(1-8)-phospholipase A2 (L-fragment), which shows only greatly reduced activity, was reduced and oxidized but no effective reformation of the native structure was found, indicating that the entire sequence is required for efficient reorganization.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002942:Circular Dichroism; D003435:Crotalid Venoms / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D010084:Oxidation-Reduction; D010740:Phospholipases / Q000302:isolation & purification; D010741:Phospholipases A / Q000302:isolation & purification; D054467:Phospholipases A2; D013438:Sulfhydryl Compounds / Q000032:analysis
D003435:Crotalid Venoms; D013438:Sulfhydryl Compounds; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2