Crotoxin. I. Immunology and interaction of the subunits.
p6ospholipase A and crotapotin were purified by gel filtration and ion-exchange chromatography from Crotalus durissus terrificus venom. The minimal molecular weights obtained by amino acid analysis were 13,400 daltons for phospholipase A and 8,300 daltons for crotapotin. Phospholipase A and crotapotin form a 1:1 molar complex that was stable during gel filtration. The LD50 of phospholipase A was increased about 10-fold when in a 1:1 complex with crotapotin. Crotapotin, phospholipase A and crotoxin (crotapotin + phospholipase A) gave precipitin reactions when tested with specific rabbit antisera. Immunological studies of these proteins using crotalus antiserum confirmed 1:1 molar ratio of the complex. Crotoxin antiserum also reacts with either crotapotin or phospholipase A separately. Similar antigenic determinants were observed in the subunits and it seems that some antigenic determinants are masked in the phospholipase A-crotapotin complex.
D000941:Antigens / Q000276:immunology; D002850:Chromatography, Gel; D003429:Cross Reactions; D003435:Crotalid Venoms / Q000378:metabolism*; D003439:Crotoxin / Q000276:immunology / Q000378:metabolism*; D004347:Drug Interactions; D004586:Electrophoresis; D007106:Immune Sera / Q000276:immunology; D007457:Iodine Radioisotopes; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000276:immunology / Q000378:metabolism*; D011863:Radioimmunoassay
D000941:Antigens; D003435:Crotalid Venoms; D007106:Immune Sera; D007457:Iodine Radioisotopes; D003439:Crotoxin; D010740:Phospholipases; D010741:Phospholipases A