Isolation of a plasma protein elevated during hypertension in the rat.
Plasma from male rats contains a protein that is elevated during essential hypertension. This protein, termed hypertension associated protein (HAP), can be detected as a peptide that has a molecular weight of 14,000 daltons on high resolution SDS-gradient polyacrylamide gels. The native protein has now been isolated by elution from DEAE-Sepharose, carboxymethyl cellulose and by gel permeation on Ultrogel AcA44. The procedure yields 102 mg of highly purified protein from 5 ml (250 mg) of plasma in 72 h. The native protein has a molecular weight of 28,000 daltons.
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006973:Hypertension / Q000097:blood*; D008297:Male; D008970:Molecular Weight; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D001798:Blood Proteins