Characterization of gangliosides by direct inlet chemical ionization mass spectrometry.
Permethylated derivatives of N-acetylneuraminic acid-containing GM3, N-glycolylneuraminic acid-containing GM3, GD1a, and GD1b, were analyzed by direct inlet ammonia chemical ionization (CI) mass spectrometry. These compounds were found to yield simple fragmentations, prominent fragment ions in the high mass region, and characteristic fragment ions corresponding to the cleavage of glycosidic linkages. This method also provides detailed information on the carbohydrate sequence and lipophilic composition in gangliosides.
D000311:Adrenal Glands / Q000032:analysis; D000313:Adrenal Medulla / Q000032:analysis; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D005227:Fatty Acids / Q000032:analysis; D005679:G(M3) Ganglioside / Q000032:analysis; D005732:Gangliosides / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D009438:Neuraminic Acids / Q000378:metabolism; D012794:Sialic Acids / Q000378:metabolism
D005227:Fatty Acids; D005679:G(M3) Ganglioside; D005732:Gangliosides; D009438:Neuraminic Acids; D012794:Sialic Acids; C032592:N-glycolylneuraminic acid