[Biological effects of smog. VIII. Impulse cytophotometric cell cycle analysis of synchronized Syrian hamster kidney cell cultures (line 14-b)].
Syrian hamster kidney cultures of line 14-1b were synchronized by excess of thymidine. Thereafter in the phase of DNA synthesis cell cultures were exposed to extract and fractions of city smog, derived from a polluted area at the river Rhine and Ruhr. Using impulscytophotometry and estimation of mitotic frequency cell cycle analyses were conducted on synchronized exposed and control cultures. The total extract obtained by methanol treatment of city smog was further fractionated by organic solvents leading to fractions of cyclohexane, polyaromates and propanol. Cell cycle progression of synchronized cultures was inhibited in a dose dependent manner by increasing concentration of city smog extract and its fractions. This inhibition led to a prolongation of DNA synthetic phase and to an accumulation of cells in G2(+ M)-phase. The total cell cycle showed a prolongation of 3-5 h. The strongest effect was induced by the total extract. We have to emphasize that already amounts of city smog which were found in air volumes of 2-5 m3 exerted strong effects. With a declining sequence of toxicity followed the fractions of cyclohexane, propanol and of polyaromates. Our results show, that samples of city smog from polluted areas contain substances which induce heavy alterations in cell cycle progression of mammalian cells in vitro. These highly effective toxic substances are dangerous for human health, especially after a long-time exposition.
D000433:1-Propanol / Q000633:toxicity; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D006224:Cricetinae; D003510:Cyclohexanes / Q000633:toxicity; D004247:DNA / Q000378:metabolism; D005434:Flow Cytometry; D066298:In Vitro Techniques; D007668:Kidney; D008647:Mesocricetus; D011083:Polycyclic Compounds / Q000633:toxicity; D012905:Smog / Q000009:adverse effects* / Q000032:analysis
D003510:Cyclohexanes; D011083:Polycyclic Compounds; D012905:Smog; D004247:DNA; D000433:1-Propanol
Biologische Wirkung von atmosphärischen Feinstäuben. VIII. Impulszytophotometrische Zellzyklusanalysen an synchronisierten Kulturen syrisher Hamsternierenzellen (Linie 14-1b).