A rapid internucleotide bond formation for the phosphotriester approach by use of new condensing reagents.
Bis(2,4,6-trihalophenyl) phosphorochloridates (TCP and TBP) were found to be effective as condensing reagents for the rapid internucleotidic phoshotriester bond formation. The benzoyl group as the N3-imide protecting group of thymidine was also useful for prevention of the side reaction caused by the active condensing reagents.
D055598:Chemical Phenomena; D002621:Chemistry; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D010755:Organophosphates; D010720:Phosphines; D013329:Structure-Activity Relationship
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D010755:Organophosphates; D010720:Phosphines; C044280:bis(2,4,6-trichlorophenyl) phosphorochloridate; C044281:bis(2,4,6-tribromophenyl) phosphorochloridate