Endogenous activation by indirect muta-carcinogens and DNA-repair synthesis in mouse-lung fibroblast cultures.
Cultures of adult mouse-lung fibroblasts have been treated by series of strong and weak muta-carcinogens. Unscheduled DNA synthesis has been measured by quantitative autoradiography using automatic image analysis. Some of the muta-carcinogens (AFB1, DMN, B(a)P, DMBA, MNNG, 4-NQO) yielded a measurable UDS response, whereas others (2-AA, AFB2, B(e)P, ICR-191) usually known as weak carcinogens, gave no response. The response was not improved by addition of liver S9. This shows that mouse-lung fibroblasts possess their own but limited metabolic activation systems.
D000818:Animals; D001711:Biotransformation; D002273:Carcinogens / Q000494:pharmacology*; D002478:Cells, Cultured; D004260:DNA Repair; D004261:DNA Replication / Q000187:drug effects*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008862:Microsomes, Liver / Q000378:metabolism; D009153:Mutagens / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D002273:Carcinogens; D009153:Mutagens