A putative primer for second-strand DNA synthesis of maize streak virus is virion-associated.
We have isolated, from maize streak virus (MSV) preparations, a population of 'nested' DNA molecules. These molecules have ribonucleotides covalently linked to the DNA species' discrete 5' deoxyribonucleotide terminus. The major species has a DNA sequence of 80 nucleotides which is complementary to a region 5' of two hairpin structures on the MSV genome, almost exclusively in an intergenic region. These molecules have been used to prime the synthesis in vitro of a complementary strand to virion DNA, initiating this reaction at one site on the genome.
D001483:Base Sequence; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis*; D010942:Plant Viruses / Q000378:metabolism*
D004277:DNA, Single-Stranded; D004279:DNA, Viral