Species-specific detection of Mycoplasma hyorhinis using DNA probes.
Two specific DNA fragments from a genomic library of Mycoplasma hyorhinis demonstrated species-specific Southern and dot hybridization using a panel of different mycoplasmas. These probes selectively recognized M. hyorhinis sequences in purified DNA, broth-grown organisms, and infected cell cultures, providing a direct method for differential detection of this mycoplasma species without prior purification of the organism.
D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D009174:Mycoplasma / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D013045:Species Specificity
D004269:DNA, Bacterial