Development of an enzyme-based assay for acetaminophen.
A new and novel method for determination of serum acetaminophen is described. The assay, which can be completed in less than 5 min, is based on the enzymatic hydrolysis of acetaminophen, with subsequent colorimetric detection of the aminophenol so produced. Various possible means of aminophenol estimation are described; the final reaction conditions have been optimized for maximum sensitivity and assay speed. This assay compares favorably with other available procedures; it requires only small sample volumes; it is rapid, simple, and highly specific for the parent drug; and it requires neither great technical ability nor expensive instrumentation.
D000082:Acetaminophen / Q000097:blood*; D000581:Amidohydrolases; D000627:Aminophenols / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D003124:Colorimetry; D006801:Humans; D006868:Hydrolysis; D007202:Indicators and Reagents; D013053:Spectrophotometry; D013607:Tablets
D000627:Aminophenols; D007202:Indicators and Reagents; D013607:Tablets; D000082:Acetaminophen; D000581:Amidohydrolases; C019732:aryl acylamidase; C026729:4-aminophenol