Effect of surfactants on the separation of proteins by reversed-phase high-performance liquid chromatography. I. Non-ionic surfactants (Tween).
The retention of a number of proteins on diphenyl-bonded silica coated with non-ionic surfactants (Tween) was investigated. It was found that a hydrophilic surface is formed by the interaction of Tween with the diphenyl groups on the packing surface. The hydrophilic stationary phase generated can be used for the size-exclusion chromatography of proteins.
D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D017830:Octoxynol; D011092:Polyethylene Glycols; D011136:Polysorbates; D011506:Proteins / Q000302:isolation & purification*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents
D002021:Buffers; D011136:Polysorbates; D011506:Proteins; D012997:Solvents; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D017830:Octoxynol