Chemical synthesis and biological activities of partially inosine-substituted 2',5'-oligoadenylates: functional discrimination of three adenine amino groups on 2-5A for the binding to and activation of 2-5A-dependent endonuclease.
Binding and activation efficacies to the 2-5A-dependent endonucease by chemically synthesized partially inosine-substituted 2-5A analogs, namely, pppI2'p5'A2'p5'A, pppA2'p5'I2'p5'A and pppA2'p5'A2'p5'I were compared with that of native 2-5A in mouse L cell and human lymphoblastoid cell extracts. The results obtained in this study indicated that the first adenine amino group from the 5' terminus of 2-5A molecule plays critical role in binding to the endonuclease, whereas the third adenine amino group has a function for the activation of this enzyme.
D000227:Adenine Nucleotides / Q000138:chemical synthesis* / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D004722:Endoribonucleases / Q000378:metabolism*; D004789:Enzyme Activation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007288:Inosine / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000494:pharmacology; D007739:L Cells / Q000378:metabolism; D051379:Mice; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000494:pharmacology; D013329:Structure-Activity Relationship
D000227:Adenine Nucleotides; D007202:Indicators and Reagents; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D007288:Inosine; C023505:2',5'-oligoadenylate; C033547:2'-5'-oligo(A)-dependent endonuclease; D004722:Endoribonucleases