title: Polyethylene glycol-modified catalase exhibits unexpectedly high activity in benzene.
abstract: Bovine liver catalase with molecular weight of 248,000, which consists of four subunits, was modified with 2,4-bis(o-methoxypolyethylene glycol)-6-chloro-s-triazine(activated PEG2). The modified catalase became soluble in organic solvents such as benzene by increasing the degree of modification of amino groups in the enzyme with activated PEG2. The enzymic activity of the modified catalase in benzene, in which 42% of the total amino groups were coupled with the modifier, was unexpectedly high in comparison with the activity of non-modified catalase in aqueous system. The absorption spectrum of the modified catalase in benzene showed the characteristic pattern of a haem protein with Soret band at 405 nm. The temperature-activity profile of the modified catalase in benzene was clarified and its activation energy was estimated to be 1900 cal/mol.
mesh_terms: D000818:Animals; D001554:Benzene; D002151:Calorimetry; D002374:Catalase / Q000378:metabolism*; D002417:Cattle; D007202:Indicators and Reagents; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012995:Solubility; D012997:Solvents; D013053:Spectrophotometry
chemical_list: D007202:Indicators and Reagents; D046911:Macromolecular Substances; D012997:Solvents; D011092:Polyethylene Glycols; C029761:2,4-bis(O-methoxypolyethylene glycol)-6-chloro-s-triazine; D002374:Catalase; D001554:Benzene