Isolation and characterization of nutritionally variant streptococci from animal sources.
Ten nutritionally variant streptococci were recovered from clinical specimens submitted to the Kansas State University Veterinary Clinical Bacteriology laboratory over a 4-year period. Isolates were recognized visually on primary blood agar plates by their satellite growth around a previously overlaid Staphylococcus aureus culture. All isolates grew within 24 hours in Todd-Hewitt and heart infusion broths supplemented with 5% bovine fetal serum and 5% S aureus filtrate. They also grew anaerobically in supplemented broths within 48 hours. However, isolates did not grow aerobically or anaerobically in the absence of supplements up to a 7-day postinoculation period. As determined by the standard Kirby-Bauer technique, the isolates were highly susceptible to antimicrobial agents commonly recommended in veterinary medicine. The isolates did not react with the corresponding Lancefield group-specific antisera, as tested by the capillary precipitin test.
D000693:Anaerobiosis; D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000942:Antigens, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial; D014644:Genetic Variation; D012703:Serotyping; D013291:Streptococcus / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
D000900:Anti-Bacterial Agents; D000942:Antigens, Bacterial