[New isolation procedure for swine kidney acylase. Kinetics of Co2+, Mn2+, Ni2+ and Cd2+-enzymes].
A new procedure for the isolation of highly purified acylamino acid amidohydrolase from hog kidney is described which allows the preparation of the enzyme with a recovery of about 45%, a 200 fold purification and a spec. activity of 350-500 U. The essential Zn2+ of the enzyme was exchanged for Co2+, Ni2+, Mn2+ and Cd2+, and the kinetic parameters KM, kcat and kcat/KM of the different enzyme species for a series of acetyl-L-amino acids were determined.
D000581:Amidohydrolases / Q000378:metabolism*; D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D002413:Cations, Divalent; D003035:Cobalt / Q000494:pharmacology; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008345:Manganese / Q000494:pharmacology; D009532:Nickel / Q000494:pharmacology; D013379:Substrate Specificity; D013552:Swine
D002413:Cations, Divalent; D002104:Cadmium; D003035:Cobalt; D008345:Manganese; D009532:Nickel; D000581:Amidohydrolases
Ein neues Isolierungsverfahren für Schweinenierenacylase. Kinetik des Co2+, Mn2+, Ni2+ und Cd2+-enzyms.