Fractionation of human serum proteins by immobilized metal affinity chromatography.
Human serum was fractionated on zinc and cadmium iminodiacetate and tris(carboxymethyl)ethylenediamine chelates. Zinc iminodiacetate was found to be useful in the isolation of hemopexin and alpha 2-macroglobulin. The chromatographic behaviour of serum proteins on zinc iminodiacetate could be modulated by the nature of the eluent; a sulphate-containing solvent increased the binding of serum proteins to immobilized metal ions.
D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000302:isolation & purification*; D002614:Chelating Agents; D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008670:Metals; D013696:Temperature
D001798:Blood Proteins; D002614:Chelating Agents; D008670:Metals