[Capacity for differentiation and normalization of tumor cell populations transplanted into the anterior chamber. IV. Ehrlich ascites carcinoma].
The activity of LDH M- and H-forms, nuclear DNA contents and of genome mutation rates in the Ehrlich ascitic carcinoma cells were studied after transplantation to the intraperitoneal cavity, subcutaneous connective tissue (SCT) and the eye anterior chamber (EAC). SCT and EAC cultivations demonstrate the increase of M- to H-forms activity ratio due to a lesser H-form activity; so, LDH profiles appear to be associated with such a spontaneously highly differentiated adenocarcinoma of murine breast cancer. SCT and EAC cultivation leads also to changes in the karyotypic structure of cell population--there are no polyploid cells (DNA content is more than 4c). It may result from a sharp fall in genome mutation rates in the Ehrlich ascitic carcinoma clones following EAC proliferation which appears 47 times lower than those during lung proliferation. The data obtained favor a hypothesis that the increase in differentiation level and decrease in tumorigenety of cancer cells during EAC proliferation may be due to selection of the near-diploid cells and to the reduction in genome mutation rates, which in the whole results in decreasing genotypic and epigenotypic variability in tumor cell populations.
D000818:Animals; D000867:Anterior Chamber; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D002471:Cell Transformation, Neoplastic / Q000378:metabolism / Q000473:pathology*; D002999:Clone Cells / Q000201:enzymology / Q000473:pathology; D004273:DNA, Neoplasm / Q000378:metabolism; D005134:Eye Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D005787:Gene Frequency; D006651:Histocytochemistry; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D009368:Neoplasm Transplantation
D004273:DNA, Neoplasm; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase
Sposobnost' k differentsirovke i normalizatsii populiatsiĭ opukholevykh kletok pri transplantatsii v peredniuiu kameru glaza. IV. Astsitnaia kartsinoma Erlikha.