[Continuous measurement of the catalytic activity of adenosine deaminase using the pH stat method].
The enzymatic deamination of adenosine to inosine produces ammonia, which causes a pH-increase of the reaction mixture. The pH-stat method is based on the continuous addition of protons to keep the pH at a constant value. The theoretical principles are discussed. The quantitative limits of the assay and the effect of changing the pH were investigated. A correction factor was derived and calculated for the pH range 6.4 to 8.5. This sensitive method allows the continuous recording of the adenosine deaminase activity in strongly coloured or turbid biological samples.
D000243:Adenosine Deaminase / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008722:Methods; D009700:Nucleoside Deaminases / Q000032:analysis*
D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase
Kontinuierliche Messung der katalytischen Aktivität von Adenosindesaminase mit der pH-Stat-Methode.