A new microspectrophotometric method for measuring absorbance of rat photoreceptors.
A method has been developed for obtaining visual pigment absorbance spectra from fixed, frozen sections of the albino rat retina. The retinas are transversely sectioned at various thicknesses on a cryo-microtome and the resulting slices are examined with a photon-counting microspectrophotometer. Problems caused by the limited pigment content of small receptors are reduced and measurements can be made at well-specified locations along the retinal section. This method is both precise and accurate and permits comparisons across sections and across animals.
D000818:Animals; D005629:Frozen Sections; D008867:Microtomy; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000032:analysis*; D012243:Rhodopsin / Q000032:analysis; D012374:Rod Cell Outer Segment / Q000032:analysis; D013053:Spectrophotometry / Q000379:methods*
D012243:Rhodopsin