Forelimb malformation in rats caused by cyclophosphamide.
A single dose of cyclophosphamide (20 mg/kg) was administered to female rats on Day 12 of gestation. Fetuses were collected at 24-h intervals from gestation Day 18 to 20. Gross malformations of the forelimb were observed, notably micromelia, oligodactyly, brachydactyly and adactyly. Specimens stained with Alizarin red-s and Alcian blue revealed postaxial skeletal deformities. Chronological histochemical investigations revealed delay in the ossification of the radius and completely unossified ulna; the end results showed the ulna to be more affected than the radius.
D000014:Abnormalities, Drug-Induced / Q000209:etiology* / Q000473:pathology; D000818:Animals; D003520:Cyclophosphamide / Q000633:toxicity*; D005260:Female; D005552:Forelimb / Q000002:abnormalities*; D005865:Gestational Age; D010012:Osteogenesis / Q000187:drug effects; D011247:Pregnancy; D011884:Radius / Q000002:abnormalities*; D051381:Rats; D014457:Ulna / Q000002:abnormalities*
D003520:Cyclophosphamide