A sensitive enzymatic assay for determination of cholesterol in lipid extracts.
A procedure for the determination of free and total cholesterol in lipid extracts is described. The method for free cholesterol employs cholesterol oxidase to generate H2O2 and peroxidase to catalyze the reaction of H2O2 with o-dianisidine to yield a colored product. For the determination of total cholesterol, cholesterol ester hydrolase is included.
D000818:Animals; D001923:Brain Chemistry; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D002784:Cholesterol / Q000032:analysis*; D002788:Cholesterol Esters / Q000032:analysis; D002789:Cholesterol Oxidase; D006735:Horseradish Peroxidase; D007700:Kinetics; D008055:Lipids / Q000032:analysis*; D017830:Octoxynol; D011092:Polyethylene Glycols; D051381:Rats; D013053:Spectrophotometry; D002787:Sterol Esterase; D013552:Swine
D002788:Cholesterol Esters; D008055:Lipids; D011092:Polyethylene Glycols; D017830:Octoxynol; D002784:Cholesterol; D002789:Cholesterol Oxidase; D006735:Horseradish Peroxidase; D002787:Sterol Esterase