title: Lipopolysaccharide and serum synergistically stimulate ornithine decarboxylase in Chinese hamster ovary cells.
abstract: Lipopolysaccharide (LPS), the active component of bacterial endotoxin, caused no significant increase in ornithine decarboxylase (ODC) activity in serum-starved, Chinese hamster ovary fibroblasts. However, concurrent addition of LPS with 10% fetal bovine serum caused a synergistic 30 to 40-fold increase in enzyme activity as compared to the 10 to 20-fold increase seen after addition of serum alone. This synergism was not due to an alteration in the time course of enzyme induction after serum addition. The LPS-induced synergy of ODC induction by serum was inhibited by the concurrent addition of the specific LPS-antagonist, Polymyxin B.
mesh_terms: D000818:Animals; D001790:Blood Physiological Phenomena; D006224:Cricetinae; D003412:Cricetulus; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010053:Ovary / Q000166:cytology* / Q000201:enzymology
chemical_list: D008070:Lipopolysaccharides; D009955:Ornithine Decarboxylase