[Observation of filaments of 10nm in the demembraned cytoplasm of Amoeba proteus].
We report here the first observation of 10 nm filaments in a protozoan, Amoeba proteus. These intermediate sized filaments were observed in spread cytoplasmic preparations of amoeba as stable cytoplasmic components over a wide range of pH (5.0-9.0). Although their morphology is grossly similar to the vertebrate intermediate filaments by negative staining, the filaments of amoeba show a characteristic helical structure with a 25 nm axial periodicity and do not display fibrillar projection along their length or at their extremity.
D000656:Amoeba / Q000648:ultrastructure*; D000818:Animals; D002458:Cell Fractionation; D002462:Cell Membrane / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D003599:Cytoskeleton / Q000648:ultrastructure*; D008854:Microscopy, Electron
Observation de filaments de 10 nm dans le cytoplasme démembrané d'Amoeba proteus.