title: High tyrosine protein kinase activities in soluble and particulate fractions in bone marrow cells.
abstract: High tyrosine protein kinase activities were detected in soluble and particulate forms from bone marrow cells using synthetic peptide (Glu-Asp-Ala-Glu-Tyr-Ala-Ala-Arg-Arg-Arg-Gly) as a substrate. Total activity of tyrosine protein kinase was 2.4 times higher in the soluble fraction but the specific activity was slightly higher in the particulate one. Mg2+ or Mn2+ requirements of these two enzymes for maximal activity were quite different from each other. Physiological significance of these two forms of enzymes is briefly discussed.
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000201:enzymology*; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D008297:Male; D008345:Manganese / Q000378:metabolism; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D011494:Protein Kinases / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
chemical_list: D011092:Polyethylene Glycols; D008345:Manganese; D017830:Octoxynol; C010615:Nonidet P-40; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; D008274:Magnesium