Reversed-phase high-performance liquid chromatography for the determination of steroid hormones in oral contraceptives.
Reversed-phase high-performance liquid chromatography with UV detection is studied for the determination of both progestogenic and oestrogenic components of oral contraceptive formulations. The applicability of the assay is demonstrated for a number of different progestogen-oestrogen combinations in both conventional tablet and novel "paper" formulations. The results show that the method developed is a versatile technique for the routine assay of these pharmaceutical formulations.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003276:Contraceptives, Oral / Q000032:analysis*; D003278:Contraceptives, Oral, Hormonal / Q000032:analysis*; D004304:Dosage Forms; D004967:Estrogens / Q000032:analysis*; D010209:Paper; D011372:Progestins / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013607:Tablets
D003276:Contraceptives, Oral; D003278:Contraceptives, Oral, Hormonal; D004304:Dosage Forms; D004967:Estrogens; D011372:Progestins; D013607:Tablets