Determination of urinary and plasma dihydroxyphenylalanine by coupled-column high-performance liquid chromatography with C8 and C18 stationary phases.
This paper describes a rapid, simple and sensitive method for measurement of L-dihydroxyphenylalanine (DOPA) in biological fluids. The method uses a two step frontal-chromatographic clean-up, followed by coupled minibore HPLC with electrochemical detection. The use of phenylboronate removes the peaks that interfere with the detection of DOPA. Inclusion of [14C]DOPA corrects for any variability in recovery.
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007980:Levodopa / Q000032:analysis* / Q000097:blood / Q000652:urine; D008297:Male; D008875:Middle Aged
D007980:Levodopa