Thermochemiluminescent assay of porcine, rat, and human erythrocytes for antioxidative deficiencies.
The thermal induction of chemiluminescence of luminol-horseradish peroxidase-labeled erythrocytes from pigs, rats, and man was studied. The luminescent responses of rat, porcine, and human erythrocytes to heating were linear in respect to logs of counts per minute versus temperature. Landrace-Duroc crossbred pigs with a history of malignant hyperthermia (porcine stress syndrome) and Poland-China-miniature pigs inbred for malignant hyperthermia (MH) yielded erythrocytes with high-level thermochemiluminescence (TCL). Sprague-Dawley rat erythrocytes were intermediate in their production of TCL. Normal human and MH-resistant miniature swine erythrocytes produced low-level TCL. However, pretreatment of human erythrocytes with 1-chloro-2,4-dinitrobenzene (CDNB) resulted in high-level TCL. Furthermore, halothane enhanced the TCL of CDNB-treated human erythrocytes and Landrace-Duroc porcine erythrocytes that were not treated with CDNB. Red blood cells from pigs susceptible to the porcine stress syndrome demonstrated a TCL response very similar to CDNB-treated erythrocytes.
D000818:Animals; D004137:Dinitrochlorobenzene / Q000494:pharmacology; D004912:Erythrocytes / Q000201:enzymology*; D006221:Halothane / Q000494:pharmacology; D006735:Horseradish Peroxidase; D006358:Hot Temperature; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol; D008305:Malignant Hyperthermia / Q000097:blood; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D013552:Swine; D013556:Swine, Miniature
D004137:Dinitrochlorobenzene; D008165:Luminol; D006735:Horseradish Peroxidase; D006221:Halothane