Detection of N-nitrosamines in the saliva of habitual chewers of tobacco.
The saliva of habitual chewers of tobacco and of non- chewers was examined using high-performance liquid chromatography and gas-liquid chromatography-thermal energy analysis. N-Nitrosodimethylamine, N-nitrosodiethylamine, N-nitrosopyrrolidine, N-nitrosonornicotine and N- nitrosohydroxyproline were detected in the saliva from the tobacco chewers . These N-nitroso compounds are probably leached from the tobacco itself and/or are formed in situ by the nitrosation of tobacco alkaloids as well as that of secondary amines. No N-nitroso compounds were detected in the saliva of non- chewers .
D000328:Adult; D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D006358:Hot Temperature; D006801:Humans; D008297:Male; D009602:Nitrosamines / Q000302:isolation & purification*; D010947:Plants, Toxic; D012463:Saliva / Q000032:analysis*; D014026:Nicotiana
D009602:Nitrosamines