Inhibition and recovery of the replication of depurinated parvovirus DNA in mouse fibroblasts.
Apurinic sites were introduced in the single-stranded DNA of parvovirus minute-virus-of-mice (MVM) and their effect on viral DNA synthesis was measured in mouse fibroblasts. Approximately one apurinic site per viral genome, is sufficient to block its replication in untreated cells. The exposure of host cells to a sublethal dose of UV-light 15 hours prior to virus infection, enhances their ability to support the replication of depurinated MVM. Cell preirradiation induces the apparent overcome of 10-15% of viral DNA replication blocks. These results indicate that apurinic sites prevent mammalian cells from replicating single-stranded DNA unless a recovery process is activated by cell UV-irradiation.
D000818:Animals; D004261:DNA Replication / Q000528:radiation effects; D004279:DNA, Viral / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D051379:Mice; D008915:Minute Virus of Mice / Q000378:metabolism* / Q000528:radiation effects; D008954:Models, Biological; D010321:Parvoviridae / Q000378:metabolism*; D011687:Purines / Q000378:metabolism*; D014466:Ultraviolet Rays; D014779:Virus Replication
D004279:DNA, Viral; D011687:Purines