Intramammary infusions of cytochalasin B and dimethyl sulfoxide do not suppress milk secretion in the goat.
Cytochalasin B, an intracellular microfilament antagonist, was evaluated for its capacity to inhibit milk secretion in the goat. Intramammary infusions of the drug via teat canal in amounts to 6 mg had no effects on yields or fat and protein contents and minor, if any, effects on somatic cell counts of consecutive 12-h milkings. Our results suggest that the apical plasma membrane of lactating cells is impermeable to cytochalasin B and that the reduced secretion of lactose and casein caused by the drug in vitro may arise from its interference with glucose uptake at the base of cells. Dimethylsulfoxide, which we used (2 ml) in infusates to solubilize cytochalasin B, also was without effect on the foregoing lactation characteristics.
D000818:Animals; D003571:Cytochalasin B / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005260:Female; D006041:Goats / Q000378:metabolism*; D007267:Injections; D007774:Lactation / Q000187:drug effects*; D007785:Lactose / Q000378:metabolism; D050356:Lipid Metabolism; D008321:Mammary Glands, Animal; D008892:Milk / Q000166:cytology / Q000378:metabolism*; D008894:Milk Proteins / Q000378:metabolism; D011247:Pregnancy
D008894:Milk Proteins; D003571:Cytochalasin B; D007785:Lactose; D004121:Dimethyl Sulfoxide