Injection of murine embryonal carcinoma cells and embryo-derived pluripotential cells into mouse blastocysts.
Two pluripotential mouse cell lines, the OTT 6050-derived cell line TCE and the embryo-derived stem cell line BLC-1, were injected into blastocysts to analyze their developmental potential. The contribution of TCE cells to the embryo was found to be limited and sporadic. There was no indication of a preferential colonization of extraembryonal membranes or developmentally related tissues in adult chimeras. BLC-1 cells failed to colonize the embryo. This indicates that a normal karyotype, pluripotency, and cell surface markers which are shared by cells of early embryos are not necessarily sufficient markers for their ability to participate in embryogenesis.
D000818:Animals; D002460:Cell Line; D002678:Chimera; D000013:Congenital Abnormalities / Q000196:embryology; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000166:cytology*; D007267:Injections; D051379:Mice; D009368:Neoplasm Transplantation; D033581:Stem Cell Transplantation; D013724:Teratoma / Q000473:pathology*