Common conditions for high-performance liquid chromatographic microdetermination of aldoses, hexosamines, and sialic acids in glycoproteins.
All aldoses known to be present in animal and plant glycoproteins were separated in partition mode on a column of a highly crosslinked cation-exchange resin (Shodex DC-613, H+ form) and sensitively monitored by photometric detection after postcolumn labeling with 2-cyanoacetamide. Linearity for the sample amount ranging from 0.1 to 80 nmol was observed with high reproducibility. Hexosamines could be determined as their N-acetyl derivatives under the identical conditions with approximately one order of magnitude higher range of linearity. Sialic acid could be estimated as N-acylhexosamines produced by the action of N-acetylneuraminate pyruvate-lyase. The usefulness of these common conditions was demonstrated by analyzing component monosaccharides of some glycoprotein preparations.
D000447:Aldehydes / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins; D006595:Hexosamines / Q000032:analysis*; D006801:Humans; D006868:Hydrolysis; D008832:Microchemistry; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000032:analysis
D000447:Aldehydes; D002241:Carbohydrates; D006023:Glycoproteins; D006595:Hexosamines; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid