Assessment of the cataractogenic potential of cyclohexanone in guinea pigs and rabbits.
The cataractogenic potential of cyclohexanone administered intravenously (0.5 and 5.0 mg/kg) and percutaneously (0.5 ml) was assessed in guinea pigs and rabbits. The negative control article was 0.9% sodium chloride. Positive control articles for guinea pigs were iv galactose and percutaneous dimethyl sulfoxide (DMSO), and for rabbits, iv poly(I) X poly(C) and percutaneous DMSO. An untreated group of guinea pigs was used as a sham control. Animals were treated three times a week for 3 consecutive weeks. Ophthalmic examinations performed monthly for 6 months for treated animals and 7 months for untreated animals revealed the presence of anterior subcapsular vacuoles in guinea pigs in all groups. These lesions consisted of swollen lens fibers that progressed to fiber disruption and protein liquefaction. There was no statistical difference in the incidence and severity of the lesions among treatment groups. No lenticular alterations were noted in any of the rabbits treated with cyclohexanone or any other chemical. It was concluded that these alterations are, apparently, an inherent characteristic of the guinea pig, making that animal an unsuitable model for the assessment of cataractogenic potential of xenobiotics.
D000287:Administration, Topical; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002386:Cataract / Q000139:chemically induced* / Q000473:pathology; D003510:Cyclohexanes / Q000633:toxicity*; D003511:Cyclohexanols / Q000097:blood / Q000633:toxicity / Q000652:urine; D003512:Cyclohexanones / Q000097:blood / Q000633:toxicity*; D005260:Female; D005965:Glucuronates / Q000378:metabolism / Q000652:urine; D006168:Guinea Pigs; D007275:Injections, Intravenous; D007908:Lens, Crystalline / Q000473:pathology; D008297:Male; D011817:Rabbits
D003510:Cyclohexanes; D003511:Cyclohexanols; D003512:Cyclohexanones; D005965:Glucuronates; C036468:cyclohexanone