Effect of steroids on the inhibition of platelet 5HT uptake by desipramine and other antidepressants.
The inhibitory effect of desipramine on platelet 5HT uptake was tested ex vivo and in vitro in control, adrenalectomised and triamcinolone-treated rats. In both situations, the inhibitory effect of desipramine was augmented in adrenalectomised rats and decreased in triamcinolone-treated rats. These changes in desipramine sensitivity were accompanied by changes in the uptake kinetic components, Km and Vmax. In in vitro studies, the IC50 of clomipramine, fluvoxamine, zimelidine, imipramine and nomifensine was decreased in adrenalectomised rat platelets and increased in corticosterone-, dexamethasone- or triamcinolone-treated rat platelets. Deoxycorticosterone had no effect. Testosterone only affected the IC50 of desipramine and nomifensine.
D000315:Adrenalectomy; D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D001792:Blood Platelets / Q000378:metabolism*; D003891:Desipramine / Q000494:pharmacology*; D005938:Glucocorticoids / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000097:blood*; D014221:Triamcinolone / Q000494:pharmacology
D000928:Antidepressive Agents; D005938:Glucocorticoids; D014221:Triamcinolone; D012701:Serotonin; D003891:Desipramine