Phosphorylated secretory proteins from rat epididymis and their androgenic control.
Slices of the rat epididymis were incubated with [32P]orthophosphate. Analysis of the radioactive proteins in the medium by two-dimensional gel electrophoresis and autoradiography revealed 6-phosphorylated secretory proteins from the epididymis of adult rats: Mr = 62 000; 66 000; 76 000; 68 000; 19 000 and 20 000. Studies of the epididymides of immature and 7-day-castrated adult rats indicated that these phosphorylated secretory proteins were controlled by androgens.
D000728:Androgens / Q000502:physiology*; D000818:Animals; D001345:Autoradiography; D002369:Castration; D004591:Electrophoresis, Polyacrylamide Gel; D004822:Epididymis / Q000032:analysis* / Q000378:metabolism; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D008297:Male; D008970:Molecular Weight; D010766:Phosphorylation; D011506:Proteins / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D000728:Androgens; D011506:Proteins