The relationship of the rat brain 68 kDa microtubule-associated protein with synaptosomal plasma membranes and with the Drosophila 70 kDa heat-shock protein.
A protein of molecular mass 68 kDa and pI5.6 is a major translation product of rat brain mRNA [Hall, Mahadevan, Whatley, Biswas & Lim (1984) Biochem. J. 219, 751-761]. In the rat brain this protein was associated with microtubule preparations and was present together with tubulin as a component of the synaptosomal plasma membranes, synaptic vesicles and post-synaptic structures. The brain mRNA for this protein was found to hybridize specifically to the Drosophila gene for the 70 kDa heat-shock protein, thus enabling its rapid isolation.
D000818:Animals; D001923:Brain Chemistry; D002462:Cell Membrane / Q000378:metabolism; D004330:Drosophila / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D006360:Heat-Shock Proteins / Q000235:genetics*; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000378:metabolism*
D006360:Heat-Shock Proteins; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger