Soluble antibody affinity chromatography technique investigated with ultratrace [125I]thyroxine.
An amount of 10 pg of [125I]thyroxine was subjected to DEAE agarose chromatography and then complexed with an antibody. Recycling of the [125I]thyroxine now as a complex with the antibody through a second DEAE agarose column under the same conditions gave a change in the retention of the [125I]thyroxine that potentially constitutes a specific shift away from co-eluting interferences from the first column. The [125I]thyroxine was then dissociated from the antibody and subjected to a third DEAE agarose column for additional shifting of its chromatographic retention. Since the overall recovery of the [125I]thyroxine is 36%, this soluble antibody affinity technique potentially is useful for sample clean-up in labeling analysis of ultratrace solutes such as thyroxine.
D000906:Antibodies / Q000302:isolation & purification; D000937:Antigen-Antibody Reactions / Q000187:drug effects; D002846:Chromatography, Affinity / Q000379:methods; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D011092:Polyethylene Glycols / Q000032:analysis; D013974:Thyroxine / Q000032:analysis* / Q000276:immunology; D014508:Urea / Q000494:pharmacology
D000906:Antibodies; D011092:Polyethylene Glycols; D014508:Urea; D013974:Thyroxine