An induction effect of phenobarbital on rat liver microsomal 4-hydroxylation of estradiol 17-sulfate.
Induction effect of phenobarbital (80 mg/kg, daily for 4 d), 3-methylcholanthrene (40 mg/kg, daily for 4 d), and isosafrole (150 mg/kg, daily for 4 d), on 2- and 4-hydroxylations of estradiol 17-sulfate by rat liver microsomes with an NADPH-generating system was investigated. On catechol formation from estradiol 17-sulfate, only phenobarbital caused some increase of the 2-hydroxylase activity in both sexes. While 4-hydroxylase activity was caused effectively by phenobarbital and isosafrole in both sexes, especially, the induction by the former in male rats was over six-fold increase to the control.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D004958:Estradiol / Q000031:analogs & derivatives* / Q000378:metabolism; D006900:Hydroxylation; D066298:In Vitro Techniques; D008748:Methylcholanthrene / Q000494:pharmacology; D008862:Microsomes, Liver / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012451:Safrole / Q000494:pharmacology
C048615:estradiol 17-sulfate; D004958:Estradiol; D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System; D012451:Safrole; C015959:isosafrole; D010634:Phenobarbital