[Relation of the biosynthesis of intracellular and export proteins in rat liver cells during induction of proliferation by cycloheximide].
After a single injection of a sublethal dose of cycloheximide (CHI) the biosynthesis of extracellular proteins in rat hepatocytes was rapidly suppressed, the reconstitution being very slow. On the contrast, the biosynthesis of intracellular proteins (e.g., histones, and other acid-soluble liver proteins) was more resistant to CHI. The activation of biosynthesis of acid-soluble and acid-insoluble proteins was found to occur stepwise. It was assumed that the activation of synthesis and accumulation of intracellular proteins after CHI release accompanied by a decreased synthesis of extracellular proteins is one of possible causes of stimulation of DNA synthesis in the hepatocytes following a single injection of CHI.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis; D003513:Cycloheximide / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000096:biosynthesis; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis; D009685:Nuclear Envelope / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats
D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D008565:Membrane Proteins; D004247:DNA; D003513:Cycloheximide
Sootnoshenie biosinteza vnutrikletochnykh i éksportnykh belkov v kletkakh pecheni krys v protsesse induktsii proliferatsii tsikloeksimidom.