Assessment of cell-substrate adhesion by a centrifugal method.
A centrifugal method has been evaluated for measuring the strength of Vero Green Monkey kidney cell adhesion to growth surfaces. The centrifugal force necessary to remove cells gave a quantitative measure of cell adhesion and hence the quality of the growth surface. After being subjected to high gravity forces, both the remaining attached cells and the detached cells were viable, indicating the detachment process did not simply rupture the cell. Electron microscope examination of growth surfaces after cell detachment suggested that remnants related to filopodia remained.
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002498:Centrifugation / Q000379:methods; D002522:Chlorocebus aethiops; D007668:Kidney; D008855:Microscopy, Electron, Scanning; D013997:Time Factors