Sequence-dependant polymorphism of DNA duplex in solutions.
Raman spectra of six synthetic polydeoxyribonucleotide duplexes with different base sequences have been examined in aqueous solutions with different salt or nucleotide concentrations. Detailed conformational differences have been indicated between B and Z forms of poly[d(G-C)] X poly[d(G-C)], between B forms of poly[d(G-C)] X poly[d(G-C)] and poly[d(G-m5C)] X poly[d(G-m5C)], between A and B forms of poly(dG) X poly(dC), between B and "CsF" forms of poly[d(A-T)] X poly[d(A-T)], between B forms of poly[d(A-U)] X poly[d(A-U)] and poly[d(A-T)] X poly[d(A-T)], and between low- and high-salt (CsF) forms of poly(dA) X poly(dT). The Raman spectrum of calf-thymus DNA in aqueous solution was also observed and was compared with the Raman spectra of its fibers in A, B, and C forms.
D000818:Animals; D002417:Cattle; D004247:DNA; D008957:Models, Genetic; D009690:Nucleic Acid Conformation; D011089:Polydeoxyribonucleotides; D013059:Spectrum Analysis, Raman; D013329:Structure-Activity Relationship; D013950:Thymus Gland
D011089:Polydeoxyribonucleotides; D004247:DNA