title: [Calcium-dependent regulation of NAD-isocitrate dehydrogenase in the rat heart mitochondria].
abstract: Ions of Ca2+ (0.1-20 mumoles) regulated the activity of mitochondrial NAD-isocitrate dehydrogenase (isocitrate: NAD-oxidoreductase decarboxylating, EC 1.1.1.41) from rat heart altering the enzyme affinity for isocitrate. Under conditions of maximal activation by Ca2+ (20 mumoles) S0,5 was decreased from 282 down to 125 mumoles, Vmax was not altered. The calmodulin inhibitor trifluoperazine did not affect the Vmax, S0.5 and the Hill coefficient of the enzyme.
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D066298:In Vitro Techniques; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D014268:Trifluoperazine / Q000378:metabolism
chemical_list: D014268:Trifluoperazine; D007521:Isocitrate Dehydrogenase; D002118:Calcium
vernacular_title: Kal'tsiĭzavisimaia reguliatsiia NAD-izotsitratdegidrogenazy v mitokhondriiakh serdtsa krysy.