Production of a monoclonal antibody against hemorrhagic activity of Crotalus atrox (western diamondback rattlesnake) venom.
Crotalid venoms have cytotoxic properties which could be useful in medical research. Crotalus atrox venom-hyperimmunized mouse spleen cells were fused with SP2/0 myeloma cells. Forty-one wells containing the hybridoma cells were positive for C. atrox venom, as determined by the enzyme linked immunosorbent assay (ELISA). Cell line 1-e12 was cloned and used to produce ascites tumors in BALB/c mice. The monoclonal antibody produced by cloned and subcultured 1-e12 cells reacted with both C. atrox venom and six other venoms in the ELISA and neutralized the hemorrhagic activity of crude C. atrox venom. A series of monoclonal antibodies could be used in studying the nature of snake venoms.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000997:Antivenins / Q000096:biosynthesis*; D001202:Ascitic Fluid / Q000276:immunology; D002852:Chromatography, Ion Exchange; D003435:Crotalid Venoms / Q000037:antagonists & inhibitors / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006470:Hemorrhage / Q000139:chemically induced / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits
D000911:Antibodies, Monoclonal; D000997:Antivenins; D003435:Crotalid Venoms