Radioimmunoassay for MPV-295, a new antihypertensive drug.
A specific and simple radioimmunoassay for the determination of MPV-295, 4(5)-[2-(2,6-dimethylphenyl)-ethyl]imidazole, in human serum is described. The antibodies were produced in rabbits immunized with a conjugate of MPV-295 and bovine thyroglobulin prepared by diazo reaction. They were shown to be specific for MPV-295 tested against several synthetic analogues. MPV-295 was iodinated with the chloramine-T method and 125I-MPV-295 was purified in cation exchange chromatography for use as tracer. The sensitivity of the assay is 2-5 pmol/ml depending on the sample volume. Serum determinations were carried out directly with 20-50 microliter serum volumes. This assay has been used to monitor serum levels of MPV-295 in pharmacokinetic studies in healthy and hypertensive subjects.
D000906:Antibodies / Q000032:analysis; D000959:Antihypertensive Agents / Q000097:blood*; D006801:Humans; D006973:Hypertension / Q000097:blood; D007093:Imidazoles / Q000008:administration & dosage / Q000097:blood* / Q000276:immunology; D007457:Iodine Radioisotopes; D008297:Male; D011863:Radioimmunoassay / Q000379:methods*; D013954:Thyroglobulin / Q000276:immunology
D000906:Antibodies; D000959:Antihypertensive Agents; D007093:Imidazoles; D007457:Iodine Radioisotopes; C038226:4(5)-2-(2,6-dimethylphenyl)ethylimidazole; D013954:Thyroglobulin