Distribution of filipin-sterol complexes in rat hepatocytes.
Distribution of cholesterol in rat hepatocytes under normal untreated and phenobarbital-treated conditions was studied by freeze-fracturing using filipin. In untreated rat hepatocytes, filipin-sterol complexes were observed on intercellular plasma membranes as well as on lysosomes and microbodies. These complexes were evenly distributed. In phenobarbital-treated rat hepatocytes, filipin-sterol complexes in the lysosomes or microbodies were distributed unevenly. In the bile canaliculi, no filipin-sterol complexes were noted. Based on these findings, the functional state of the organellae concerned was discussed.
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D005372:Filipin / Q000032:analysis*; D008099:Liver / Q000032:analysis* / Q000648:ultrastructure; D008297:Male; D010634:Phenobarbital / Q000494:pharmacology; D011090:Polyenes / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013261:Sterols / Q000032:analysis*
D011090:Polyenes; D013261:Sterols; D005372:Filipin; D000469:Alkaline Phosphatase; D010634:Phenobarbital