Interaction of secretory protein precursors with phospholipids in liposomes.
The precursors of secretory proteins were synthesized in a reticulocyte lysate system programmed with rat serum albumin or human placental lactogen mRNA and their interaction with phospholipids in liposomes was studied. The precursor proteins could bind to acidic phospholipids that have an exposed phosphate such as dicetyl phosphate and phosphatidic acid or a phosphate that is covered by a small moiety such as phosphatidylglycerol. The binding of precursor proteins was dependent on the mol% of acidic phospholipids in lecithin-liposomes, increased with elevation of temperature in the range of 0 to 45 degrees C, and was not inhibited by the addition of a large excess of mature proteins. Mature proteins or proalbumin showed no significant binding to the liposomes containing acidic phospholipids. About 15% of the acid-precipitable radioactivity bound to the liposomes was resistant to protease digestion. This radioactivity was shown to correspond to methionine-containing peptides with molecular weights of 2,500 to 3,500. These results indicate that the post-translational insertion of a small part of the precursor proteins into the membrane did occur with the present model system, but the post-translational transfer of precursor proteins across the membrane did not.
D000818:Animals; D002474:Cell-Free System; D005260:Female; D006801:Humans; D007700:Kinetics; D008081:Liposomes; D010713:Phosphatidylcholines / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D010928:Placental Lactogen / Q000235:genetics*; D011247:Pregnancy; D011485:Protein Binding; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D051381:Rats; D012156:Reticulocytes / Q000378:metabolism*; D012709:Serum Albumin / Q000235:genetics*; D013816:Thermodynamics
D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; D011498:Protein Precursors; D012333:RNA, Messenger; D012709:Serum Albumin; D010928:Placental Lactogen