title: [Isolation and characteristics of the fibroblast cell line of rats cotransformed by the DNA of simian adenovirus SA7 (C8) and hepatitis B virus].
abstract: Transformed cell line (SH2) was established by means of co-transfection of primary rat embryonal fibroblasts by DNA of high-oncogenic simian adenovirus SA7 (C-8) and hepatitis B virus. SH2 cells possess a transformed phenotype and high oncogenicity both for allogenic (rats) and xenogenic (hamsters) animals. 100 SH2 cells induce tumours in newborn (3 day) hamsters. 10(4) SH2 cells inoculated to adult hamsters induce tumours in approximately 50% cases on the 20th-30th day. Possible mechanisms of significant stimulation of SH2 cells oncogenic properties after co-transfection by DNA of oncogenic adenovirus SA7 and hepatitis B virus are discussed.
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000261:Adenoviruses, Simian / Q000235:genetics* / Q000276:immunology; D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics / Q000302:isolation & purification; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D006224:Cricetinae; D004279:DNA, Viral / Q000235:genetics*; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000382:microbiology; D006515:Hepatitis B virus / Q000235:genetics* / Q000276:immunology; D009368:Neoplasm Transplantation; D010641:Phenotype; D051381:Rats; D011916:Rats, Inbred F344; D014162:Transfection
chemical_list: D000957:Antigens, Viral, Tumor; D004279:DNA, Viral
vernacular_title: Poluchenie i kharakteristika linii kletok fibroblastov krysy, kotransformirovannykh DNK adenovirusa obez'ian SA7 (C8) i virusa gepatita B.