Counter current immunoelectrophoresis, a new technique for the rapid serodiagnosis of porcine cysticercosis.
Counter current immunoelectrophoresis was used to detect porcine cysticercosis and water soluble extracts of scolex and of scolex with cyst wall were used as antigens. Serum samples from 40 pigs infected with Cysticercus cellulosae, five infected with C. tenuicollis and five with hydatid cysts, and 15 healthy pigs were tested. A sharp and thick concave precipitin band was observed at the point of interaction of antigen and antibody within 90 min in 39 sera from infected pigs (97.5%). The precipitin reaction was better in barbitone buffer of pH 8.6 with the well distance at 6 mm. No false or cross reaction were found and the test was rapid and sensitive.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000276:immunology; D001462:Barbital; D001881:Borates; D002021:Buffers; D003378:Counterimmunoelectrophoresis; D003551:Cysticercosis / Q000662:veterinary*; D003552:Cysticercus / Q000276:immunology; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D007122:Immunoelectrophoresis
D000906:Antibodies; D000947:Antigens, Helminth; D001881:Borates; D002021:Buffers; D001462:Barbital