title: High-performance liquid chromatographic-fluorometric determination of cinnamaldehyde in perfume, cologne and toilet water.
abstract: A high-performance liquid chromatographic (HPLC)-fluorometric method is described for the determination of trans-cinnamaldehyde in fragrances. The fragrance is added to isooctane and extracted with an aqueous solution of the sodium salt of 6-aminocaproic acid to isolate the aldehyde fraction. After dilution with water, an aliquot of the extract is added to a solution of 1,2-diaminonaphthalene monosulfate in dilute formic acid. The fluorescent derivative of cinnamaldehyde, 2-styrylnaphth[1,2-d]imidazole, is prepared by incubating and then cooling the solution and adding pyridine. Aliquots of the fluorophore solution are analyzed on a reversed-phase C18 HPLC column by using a buffered tetrahydrofuran-water eluent. Cinnamaldehyde is quantitated by comparing fluorescence emission intensity with that of a standard. Recoveries from samples of various commercial fragrances, spiked with cinnamaldehyde at the 0.01, 0.05 and 0.1% levels, ranged from 94 to 112% with a mean of 103% and a standard deviation of 5.3. The limit of detection is approximately 1 ng.
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives / Q000032:analysis*; D000447:Aldehydes / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D010476:Perfume / Q000032:analysis*
chemical_list: D000447:Aldehydes; D010476:Perfume; D000171:Acrolein; C012843:cinnamaldehyde