title: Presence of a phospholipase A2 inhibitor in porcine serum.
abstract: Porcine pancreatic phospholipase A2 (PLA2) was immobilized to Sepharose 4B and porcine serum was passed through this affinity column. Bound substances were eluted by an EDTA-containing buffer and fractionated in a Sepharose 6B column. A single protein peak of the eluate from the latter column was found to inhibit PLA2 activity in a dose-dependent manner in an assay system using radioactive lecithin as a substrate and porcine pancreatic PLA2 as the enzyme source. The serum fraction containing the PLA2 inhibitory protein(s) (PIP) appeared inhomogeneous on SDS-polyacrylamide gel electrophoresis with two major bands close to each other, corresponding to a molecular weight of approximately 60,000. It was concluded that PIP might act as a protective principle against autodigestion in acute pancreatitis and other inflammatory diseases as well as playing a regulatory role in prostaglandin metabolism.
mesh_terms: D000818:Animals; D017302:Annexins; D001798:Blood Proteins / Q000302:isolation & purification*; D002135:Calcium-Binding Proteins; D006023:Glycoproteins / Q000097:blood; D007700:Kinetics; D008970:Molecular Weight; D010740:Phospholipases / Q000037:antagonists & inhibitors*; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D013552:Swine
chemical_list: D017302:Annexins; D001798:Blood Proteins; D002135:Calcium-Binding Proteins; D006023:Glycoproteins; C030958:lipomodulin; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2