Spectrofluorimetric determination of desoxycholic acid in the presence of cholic and chenodesoxycholic acids by use of Ce(IV) and concentrated sulphuric acid.
A method for the quantitative estimation of desoxycholic acid (200-700 micrograms/ml) in the presence of cholic and chenodesoxycholic acids is described. The method is based on the transformation of desoxycholic acid in fluorescent products (lambda ex = 350 nm, lambda em = 458 nm) by the action of concentrated sulphuric acid, this being enhanced by the presence of Ce(IV). The sample is mixed with a solution of Ce(IV) and concentrated sulphuric acid under standard conditions. Fluorescence is measured in relation to a reference containing the same components except Ce(IV). Cholic and chenodesoxycholic acids do not produce a reaction under adequate conditions. Synthetic samples of bile acids were tested for validation of the method.
D002563:Cerium; D002635:Chenodeoxycholic Acid / Q000032:analysis; D019826:Cholic Acid; D002793:Cholic Acids / Q000032:analysis*; D003840:Deoxycholic Acid / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013464:Sulfuric Acids
D002793:Cholic Acids; D013464:Sulfuric Acids; D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; D002563:Cerium; C030583:ceric oxide; D019826:Cholic Acid