Determination of benperidol in human plasma by high-performance liquid chromatography.
A high-performance liquid chromatographic method for the quantitative determination of benperidol in human plasma using haloperidol as internal standard is described. The method involves liquid-liquid extraction, separation of the substances on a reversed-phase column C18 followed by ultraviolet detection at 254 nm. The mobile phase consists of 32% acetonitrile in 0.05 M potassium dihydrogen phosphate buffer (pH 2.8). The detection limit is 0.5-1.0 ng/ml using 2- or 4-ml plasma samples.
D001544:Benperidol / Q000097:blood*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006220:Haloperidol / Q000097:blood; D006801:Humans; D007700:Kinetics; D012015:Reference Standards; D014272:Trifluperidol / Q000097:blood
D001544:Benperidol; D006220:Haloperidol; D014272:Trifluperidol