Human testicular cell suspensions in vitro using post mortem material.
A method is described for culturing human testicular cells from post mortem material for at least 10 days in a serum free medium. The success of the technique is based on the fact that a layer of testicular cells remains undisturbed on the bottom of a culture flask during culturing and the medium is constantly renewed by a perfusion flow. It is shown that cells form junctions within several hours allowing metabolic coupling, indicating active survival of cells during culture period.
D000328:Adult; D000368:Aged; D000818:Animals; D001344:Autopsy; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods*; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D013737:Testis / Q000166:cytology*