Antibody to poliovirus genome-linked protein (VPg) precipitates in vitro synthesized RNA attached to VPg-precursor polypeptide(s).
Antibody to poliovirus genome-linked protein VPg, specifically precipitated RNA synthesized in vitro by the poliovirus replicase and host factor in response to poliovirion RNA. A significant amount of the immunoprecipitated RNA was RNAase T1 resistant, sedimented at approximately 2-4 S and was shown to be largely polyuridylic acid. RNAase A digestion or alkali hydrolysis of the immunoprecipitated RNA left [32P]UMP-labeled material which comigrated on SDS-polyacrylamide gels with known VPg-precursor polypeptides. The results presented in this paper suggested that VPg was involved in the host factor-dependent, poliovirus replicase-catalyzed in vitro RNA synthesis, most probably in the form of a larger precursor protein.
D000914:Antibodies, Viral / Q000276:immunology*; D011232:Chemical Precipitation; D006801:Humans; D008970:Molecular Weight; D010455:Peptides / Q000276:immunology; D012367:RNA, Viral / Q000096:biosynthesis / Q000276:immunology*; D012260:Ribonucleases / Q000494:pharmacology; D014758:Viral Core Proteins; D014764:Viral Proteins / Q000096:biosynthesis / Q000276:immunology*; D014779:Virus Replication
D000914:Antibodies, Viral; D010455:Peptides; D012367:RNA, Viral; D014758:Viral Core Proteins; D014764:Viral Proteins; D012260:Ribonucleases