title: Cation permeability and mechanical properties of the erythrocyte membrane under the influence of lysophosphatidylcholine (LPC) in isotonic and hypotonic media.
abstract: The osmotic behaviour of erythrocytes under the influence of lysophosphatidylcholine (LPC) was investigated at temperatures of +4 degrees C and 20 degrees C by allowing them either to swell rapidly in hypotonic media in the presence of LPC or to swell gradually at first and then interact with LPC. Prelytic potassium release, the degree of hemolysis and the cell volume under various osmotic conditions were measured, together with the 'returning volumes', i.e. the volumes in an isotonic solution to which the cells returned from that in the hypotonic solution. LPC had a hemolyzing effect on erythrocytes in an isotonic medium and in slightly hypotonic media under all the osmotic conditions investigated, and the degree of hemolysis increased with increasing concentrations of LPC or decreasing temperatures, being greater during gradual than during rapid swelling. LPC also produced a prelytic leakage of potassium connected with the decrease in cell volume in an isotonic medium and in 'returning volumes' in all the media and under all the osmotic conditions investigated. The semipermeability of the membrane was preserved in all these cases, however, for osmotic swelling of the erythrocytes was observed, although to a lesser extent than without LPC. During rapid swelling both the curves for the prelytic potassium leakage and the degree of hemolysis were shifted towards more dilute solutions. Since the critical hemolytic volume was not increased, the shift in potassium leakage and hemolysis caused by LPC may be due to increased rigidity in the cell membrane. The curves for both potassium leakage and hemolysis shifted towards more concentrated solutions during gradual swelling, perhaps due to increased membrane fragmentation.
mesh_terms: D002412:Cations; D002463:Cell Membrane Permeability / Q000187:drug effects*; D003470:Culture Media; D004906:Erythrocyte Count; D004907:Erythrocyte Deformability / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000187:drug effects*; D004911:Erythrocyte Volume / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D008297:Male; D009994:Osmolar Concentration; D009996:Osmotic Fragility; D011188:Potassium / Q000502:physiology
chemical_list: D002412:Cations; D003470:Culture Media; D008244:Lysophosphatidylcholines; D011188:Potassium