Flow cytometry of postmortem human testicular tissue in cases of atherosclerosis.
From forty-seven autopsy cases of atherosclerosis flow cytometry (FCM) of DNA and histology of both testes are compared with the histological sections of their supplying vessels arteriae testiculares and arteriae ductus deferentis at different levels. By this method, changes of spermatogenesis are judged separately for each side and the results can be related to the local conditions of blood supply. Four young men, dead after traffic accidents, served as control. In the majority of cases, the computer-assisted evaluations of the meiotic DNA histograms show no differences between the right and left testis, even when differences of the arterial diameters are found by histology. On the other hand, cases with distinct differences in the histograms can show insignificant pathological alterations of the vessels. Though most excessive forms of macroscopic and microscopic atherosclerosis do not necessarily lead to a significant reduction in spermatogenesis, some cases with moderate forms show a strong reduction or even a total loss. This discrepancy can best be explained by superposition of other diseases.
D000328:Adult; D000368:Aged; D001161:Arteriosclerosis / Q000473:pathology*; D001344:Autopsy; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D013737:Testis / Q000098:blood supply / Q000473:pathology*
D004247:DNA