Finding of a galactose-oxidation-product in lens of galactose-fed guinea pig.
From studies on polyols in lens of galactose-fed guinea pigs, r-galactono-1,4-lactone was found, which proves the presence of galactonic acid as a product of galactose oxidation, by gas liquid chromatography and mass spectrometry. The content of this component was one tenth of that of galactitol. In vitro culture of rat lens in 30 mM galactose-loaded media demonstrated the formation of the lactone. The significance of the lactone was discussed with respect to the galactose metabolism in lens.
D000818:Animals; D002849:Chromatography, Gas; D004040:Dietary Carbohydrates / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D006168:Guinea Pigs; D007783:Lactones / Q000032:analysis / Q000378:metabolism*; D007908:Lens, Crystalline / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D013400:Sugar Acids / Q000032:analysis / Q000378:metabolism*
D004040:Dietary Carbohydrates; D007783:Lactones; D013400:Sugar Acids; C534014:galactono-1,4-lactone; C012991:galactonic acid; C007813:galactonolactone; D005690:Galactose