A new in vitro microsuperfusion technique for investigation of human detrusor muscle.
There is a paucity of information regarding the behaviour of human detrusor muscle in vitro. This is mainly due to the dependence of muscle bath techniques on large strips of material, which can be obtained only at open operations and which may not be viable because of limited diffusion of oxygen and metabolites through the tissue. We report a new technique for the in vitro study of muscle obtained during endoscopic procedures by cup biopsy. The results obtained from normal muscle show that this technique gives consistent and reproducible results.
D001285:Atropine / Q000494:pharmacology; D002462:Cell Membrane / Q000502:physiology; D004558:Electric Stimulation; D006801:Humans; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects; D009132:Muscles / Q000502:physiology*; D010477:Perfusion / Q000379:methods*; D013779:Tetrodotoxin / Q000494:pharmacology; D001743:Urinary Bladder / Q000187:drug effects / Q000502:physiology*
D013779:Tetrodotoxin; D001285:Atropine