Conjugates of N-nitroso-tert-butylmethylamine in urine.
The glucuronide (alpha-anomer) and the mercapturic acid of N-nitroso-tert-butylhydroxymethylamine were synthesized. An analytical procedure was developed using high-performance liquid chromatography, which allowed the detection of both conjugates in a single run. A new and efficient synthesis of N-nitroso-tert-butyl-14C-methylamine was developed, based on 14C-formaldehyde. After subcutaneous administration of 23 mg/kg to a rat, the nitrosamine was metabolized to a high degree, and two conjugates were excreted in the urine.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005965:Glucuronates / Q000652:urine*; D009602:Nitrosamines / Q000652:urine*; D051381:Rats; D011919:Rats, Inbred Strains
D005965:Glucuronates; D009602:Nitrosamines; C031256:tert-butylmethylnitrosamine