[DNA-cholesterol complex studied by fluorescent probes].
DNA interaction with cholesterol at various lipid concentrations has been investigated by the fluorescent probes method. It has been shown that the intensity of acridine orange fluorescence in the DNA-cholesterol complex decreases at 24 micrograms/ml cholesterol and at 45 micrograms/ml it increases. The number of binding sites and the degree of polarization of fluorescence change simultaneously. Binary mechanism of cholesterol binding with DNA has been suggested: surface binding takes place at low concentrations, intercalation--at high lipid concentrations.
D000165:Acridine Orange / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002417:Cattle; D002784:Cholesterol / Q000494:pharmacology*; D004247:DNA / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000494:pharmacology*; D066298:In Vitro Techniques; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods
D005456:Fluorescent Dyes; D012996:Solutions; D004247:DNA; D002784:Cholesterol; D000165:Acridine Orange
Izuchenie kompleksa DNK--kholesterin metodom fluorestsentnykh zondov.