A method for preparation of dry thrombin for topical application.
Thrombin for topical hemostasis can be prepared from bovine or human blood plasma. The prothrombin is isolated by means of adsorption on DEAE-Sephadex A-50 and consecutively activated by CaCl2 and thromboplastin. Thrombin is precipitated and purified by acetone. The specific activity of the thrombin preparation is 122 + 23 IU/mg protein while the yield is 36,360 +/- 6623 IU/liter plasma.
D000287:Administration, Topical; D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D006801:Humans; D013917:Thrombin / Q000008:administration & dosage / Q000302:isolation & purification*
D013917:Thrombin