Determination of meclocycline, a tetracycline analogue, in cream formulations by liquid chromatography.
A rapid and sensitive high-performance liquid chromatographic quantitation of meclocycline (I) in a cream formulation is described. The acidified methanolic extract of the sample was diluted with mobile phase and analyzed on a reverse-phase column by using a mobile phase consisting of EDTA buffer (pH 6.6)-tetrahydrofuran (85:15 v/v). The method gave linear, quantitative, and reproducible results with a detection limit of 0.4 ppm for meclocycline.
D002853:Chromatography, Liquid / Q000379:methods; D009824:Ointments; D010118:Oxytetracycline / Q000031:analogs & derivatives* / Q000032:analysis
D009824:Ointments; C100122:meclocycline; D010118:Oxytetracycline