A human monoclonal antibody specific for tetanus toxoid.
Successful hybridization has been obtained between PWM stimulated peripheral blood lymphocytes from tetanus toxoid immunized donors and the human B lymphoma line RH-L-4. Among the hybrids selected after cloning, the average in vitro activity against tetanus toxoid was 1-5 micrograms/10(6) cells/24 hours. The specific activity of one of them was further tested in vitro (RIA) and in vivo (mouse protection test) and compared favourably with a human polyclonal antitetanus globulin.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D009500:Neutralization Tests; D013742:Tetanus / Q000517:prevention & control; D013745:Tetanus Toxoid / Q000276:immunology*
D000911:Antibodies, Monoclonal; D013745:Tetanus Toxoid