title: A method for the selective release and, hence, assay of the carbohydrate moieties of collagen.
abstract: The carbohydrate residues of collagen were selectively released in high yield by nitrosation of the hydroxylysines of the intact collagen or peptides derived from collagen. The carbohydrate residues (Glc-Gal and Gal) released were separated from the modified protein or peptide by gel chromatography and were assayed by gas-liquid chromatography of their trimethylsilyl derivatives. The results agreed closely with those obtained from methanolysis-gas chromatography or from alkaline hydrolysis followed by amino acid analysis of the hydroxylysyl glycosides. With a more sensitive perbenzoylation-high-performance liquid chromatography method and uv detection at 230 nm, the carbohydrates released by nitrosation of submilligram quantities of collagen or peptide could be assayed accurately.
mesh_terms: D000818:Animals; D001565:Benzoates; D002241:Carbohydrates / Q000032:analysis*; D002356:Cartilage / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003094:Collagen; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D000432:Methanol; D009603:Nitroso Compounds; D010455:Peptides; D051381:Rats; D013552:Swine; D013710:Tendons / Q000032:analysis; D014297:Trimethylsilyl Compounds
chemical_list: D001565:Benzoates; D002241:Carbohydrates; D009603:Nitroso Compounds; D010455:Peptides; D014297:Trimethylsilyl Compounds; D003094:Collagen; D000432:Methanol