Improved detection of calcium-binding proteins in polyacrylamide gels.
We refined the method of Schibeci and Martonosi (1980a) to enhance detection of calcium-binding proteins in polyacrylamide gels using 45Ca2+. Our efforts have produced a method which is shorter, has 40-fold greater sensitivity over the previous method, and will detect 'EF hand'-containing calcium-binding proteins in polyacrylamide gels below the 0.5 microgram level. In addition, this method will detect at least one example from every described class of calcium-binding protein, including lectins and gamma-carboxyglutamic acid containing calcium-binding proteins. The method should be useful for detecting calcium-binding proteins which may trigger neurotransmitter release.
D002118:Calcium; D002135:Calcium-Binding Proteins / Q000032:analysis*; D002147:Calmodulin / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007700:Kinetics; D011489:Protein Denaturation; D012967:Sodium Dodecyl Sulfate
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D012967:Sodium Dodecyl Sulfate; D002118:Calcium