[Immunochemical methods for measuring specific proteins].
Immunochemical methods are based on the antigen-antibody reaction. Two groups of techniques can be made: group I in which the visualization of the antigen-antibody reaction is direct (radial immunodiffusion, immunonephelometry, immunoturbidimetry...), group II which needs a label to detect the complex (radioimmuno assay, immunoenzymology). The characteristics of the principal methods (time needed, sensitivity, apparatus, applications) and their causes of error are compared. Thanks to their precision, the possibility of micromethod and their rapidity, immunonephelometric technics are recommended in pediatrics.
D000936:Antigen-Antibody Complex / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis*; D002648:Child; D003951:Diagnostic Errors; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007120:Immunochemistry; D007231:Infant, Newborn; D007553:Isotope Labeling; D008832:Microchemistry; D009391:Nephelometry and Turbidimetry
D000936:Antigen-Antibody Complex; D001798:Blood Proteins
Méthods immunochimiques de mesure des protéines spécifiques.