Analysis of DNA synthesis rate of cultured cells from flow cytometric data.
The rate of DNA synthesis along S phase is estimated from flow cytometric histograms on the basis of a mathematical model of a cell population. In the absence of loss, the model expresses the population kinetics in terms of DNA synthesis rate, S-phase influx, and population size. A single histogram is sufficient to determine the DNA synthesis rate when the population is in balanced exponential growth. Two suitably chosen histograms are necessary if the S-phase influx is exponential in a time interval longer than the S-phase duration. The analysis procedure was tested on published autoradiographic data and applied to three cultured cell lines (CM-S, 3LL, and M14 cells) that show various patterns of DNA distribution. In each case the cell-cycle fractions, the DNA synthesis rate, and the S-phase duration were obtained.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D004261:DNA Replication; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods; D007399:Interphase; D008175:Lung Neoplasms / Q000473:pathology; D008433:Mathematics; D008545:Melanoma / Q000473:pathology; D051379:Mice; D008954:Models, Biological
D004273:DNA, Neoplasm