Serological characterization of a pluripotent mouse embryonal stem cell line, two transformed derivatives, and an endoderm-like cell line.
The pluripotent mouse embryonal stem cell line BLC 1 and two transformants derived from it by DNA transformation (T1 and T2/K26) as well as the blastocyst-derived cell line BLC 3 were tested for the presence of cell surface antigens recognized by the monoclonal antibodies ECMA-7, anti-SSEA-1 and M 1/22.25, and intermediate filament proteins labeled by the monoclonal antibodies TROMA 1 and TROMA 2 using a three-step indirect immunofluorescence technique. According to present concepts and in agreement with previous data (Wobus et al., 1984a), the results obtained indicate that BLC 1, T1 and T2/K26 are undifferentiated embryonal stem cells, and BLC 3 is an endoderm-like cell line.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D002460:Cell Line; D004622:Embryo, Mammalian / Q000166:cytology / Q000276:immunology*; D004707:Endoderm / Q000166:cytology / Q000276:immunology*; D007381:Intermediate Filament Proteins / Q000276:immunology; D051379:Mice; D012699:Serology; D013234:Stem Cells / Q000166:cytology / Q000276:immunology*; D014170:Transformation, Genetic
D000954:Antigens, Surface; D007381:Intermediate Filament Proteins