[Tumorigenicity of the cell lines (TTK-1 cell lines) derived from normal human decidua in nude mice].
Two cell lines, TTK-1(E) and TTK-1(F), derived from normal human early decidual tissue have been maintained in culture through forty subcultures since July, 1979. These cell lines were transplanted subcutaneously into nude mice at 6 weeks of age to investigate their tumorigenicity. Rapidly growing tumor nodules formed at the site of implantation. The incidence of tumor growth was 60% in TTK-1(E) and 80% in TTK-1(F). The tumor tissues were composed of poorly differentiated cells arranged in cord-like and/or gland-like structures, and showed the malignant histological characteristics. Light microscopic and electron microscopic studies revealed that the properties of the tumor cell populations are identical with those of the culture cells of the two cell lines before implantation. These results obviously indicated that the tumors had grown from the implanted cells. Although the tumors developed from the respective two cell line showed some common histological features, they apparently differed in light microscopic and electron microscopic findings. Tumorigenesis with malignant features could be attributed to the in vitro spontaneous neoplastic transformations during successive subcultures, which might be useful as an in vitro model of endometrial carcinogenesis.
D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003656:Decidua; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000473:pathology*; D011247:Pregnancy