title: [Research on the anterior hypothalamic formations in tissue culture].
abstract: Using diffusion chambers and short-term tissue culture, fragments from the supraoptic nucleus of the rat hypothalamus were cultivated. The tissue culture was studied by means of morphological, histological, autoradiographic, electron-microscopic and vital-microscopic techniques. The tissue culture was shown to possess neurosecretory cells containing acetylcholinesterase and responding to acetylcholine with changes of morphometric nuclear and cytoplasm indices, with depression of the secretory protein efflux to the incubation medium, and the decrease of H3-leucine and D-xylose influx to the cells.
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D007025:Anterior Hypothalamic Nucleus / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D001345:Autoradiography; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods; D005260:Female; D007774:Lactation; D008855:Microscopy, Electron, Scanning; D009490:Neurosecretory Systems / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D011247:Pregnancy; D051381:Rats; D013495:Supraoptic Nucleus / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure
chemical_list: D000109:Acetylcholine
vernacular_title: Nekotorye aspekty izucheniia perednegipotalamicheskikh obrazovaniĭ v kul'ture tkani.