Computer-assisted analyses of [14C]2-DG autoradiographs employing a general purpose image processing system.
A general purpose image processing system is described including B/W TV camera, high resolution image processor and display system (TESAK VDC 501), computer (DEC PDP 11/23), and monochrome and color monitors. Images may be acquired from a microscope equipped with a TV camera or using the TV in direct viewing; the A/D converter and the image processor provides fast (40 ms) and precise (512 X 512 data points) digitization of TV signal with a 256 gray levels maximum resolution. Computer programs have been developed in order to perform qualitative and quantitative analyses of autoradiographs obtained with the 2-DG method, which are written in FORTRAN and MACRO 11 Assembly Language. They include: (1) procedures designed to recognize errors in acquisition due to possible image shading and correct them via software; (2) routines suitable for qualitative analyses of the whole image or selected regions of it, providing the opportunity for pseudocolor coding, statistics, graphic overlays; (3) programs permitting the conversion of gray levels into metabolic rates of glucose utilization and the display of gray- or color-coded metabolic maps.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001921:Brain / Q000378:metabolism; D002250:Carbon Radioisotopes; D003626:Data Display; D003837:Deoxy Sugars; D003847:Deoxyglucose; D001330:Electronic Data Processing; D051381:Rats
D002250:Carbon Radioisotopes; D003837:Deoxy Sugars; D003847:Deoxyglucose