Comparison of the bio-physical and biological properties of staphylococcal protein-A extracted by salt-washing and conventional procedure.
Staphylococcal protein A could be extracted in large amounts by simple stirring of the cells with physiological saline (pH 7.8). The amount of protein A obtained by the technique was found to be the same as obtained by lysozyme/lysostaphin techniques (45 micrograms/ml). The protein A extracted by salt-washing technique was of higher molecular weight (71,000) and differing in some biological properties like complement fixation from the protein A prepared by lysostaphin technique.
D000268:Adhesiveness; D003168:Complement Fixation Tests; D004591:Electrophoresis, Polyacrylamide Gel; D008248:Lysostaphin / Q000494:pharmacology; D008970:Molecular Weight; D010587:Phagocytosis; D012965:Sodium Chloride / Q000494:pharmacology; D013205:Staphylococcal Protein A / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000032:analysis* / Q000187:drug effects
D013205:Staphylococcal Protein A; D012965:Sodium Chloride; D008248:Lysostaphin