Quantitation of free and conjugated bile acids in human feces using a high-pressure liquid chromatography counterion method.
A method has been developed for extraction and purification of the major bile acids in human feces, and for their quantitative estimation using high-pressure liquid chromatography. Freeze-dried fecal material was extracted with alkaline ethanol and, after removal of neutral steroids, was subjected to thin-layer chromatography, followed by reversed-phase C18 silica cartridge (Sep-Pak) purification. The mixture was further separated into free, and glyco and tauro conjugates by ion-exchange chromatography. Subsequent resolution of individual bile acids was accomplished by HPLC using a counterion pairing method.
D001647:Bile Acids and Salts / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005243:Feces / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012997:Solvents
D001647:Bile Acids and Salts; D012997:Solvents