Monoclonal antibody (WI-2) reactive against human transformed and leukemic cells.
A monoclonal antibody (WI-2) was produced against HL-60 cells. This antibody was IgM Kappa and reacted with human cell lines derived from fibroblasts and from hemopoetic, lymphoid, and neoplastic tissues. It also reacted with lymphocytes transformed by mitogens. WI-2 lacked reactivity against normal human RBC's, mature granulocytes, and T and B lymphocytes. The target antigen for WI-2 had a molecular weight of 43,000 daltons. Specimens from patients with leukemia were tested with WI-2. The number of immature cells was compared with the number of WI-2 reactive cells in the peripheral blood and bone marrow and subjected to linear regression analysis. There was a highly significant (p less than 0.001) correlation between the two parameters. The antibody may be useful in monitoring the progress of the patients and in detecting early relapse in leukemia.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D001773:Blood Cells / Q000276:immunology; D001853:Bone Marrow / Q000276:immunology; D002460:Cell Line; D005260:Female; D006801:Humans; D006825:Hybridomas; D007938:Leukemia / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight
D000911:Antibodies, Monoclonal; D000941:Antigens