Transport of [125I] IgG and [125I] ovalbumin between lymphocyte-like and macrophage-like rat spleen cells: chromatin binding.
Spleen cells from rats were incubated for 10 min in Eagle's medium containing [125I] IgG or [125I] ovalbumin. Lymphocyte-like and macrophage-like cells were separated by centrifugation in Ficoll-metrizoate solution (d = 1.095). Control spleen cells were incubated in Eagle's medium without [125I] IgG or [125I] ovalbumin and both subpopulations were separated analogically. Lymphocyte-like cells labelled with [125I] IgG or [125I] ovalbumin were incubated for 10 min with control macrophage-like cells and labelled macrophage-like cells with control lymphocyte-like cells. After the incubation time all subpopulations were separated again in Ficoll-metrizoate solution. Radioactivity of [125I] IgG or [125I] ovalbumin was found in cytoplasmic fraction, sap protein fraction (nucleoplasm) and chromatin of control lymphocyte-like and macrophage-like cells. It may be concluded that transport of these potential antigenic compounds occurs directly in lymphocyte-like and macrophage-like cells as well as transport between these two subpopulations.
D000818:Animals; D001692:Biological Transport; D002843:Chromatin / Q000276:immunology / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism*; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D010047:Ovalbumin / Q000378:metabolism*; D051381:Rats; D013154:Spleen / Q000276:immunology / Q000378:metabolism*
D002843:Chromatin; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D010047:Ovalbumin