In vitro and in vivo demonstration of the cytoprotective effect of (+)-cyanidanol-3.
In vitro and in vivo investigations were undertaken to demonstrate cytoprotective effect of (+)-cyanidanol-3 on human erythrocytes. The time of full haemolysis induced by hydrochloride acid was measured and dynamic haemolysis curve was recorded with an aggregometer. In vitro, following a one-hour incubation of erythrocytes with (+)-cyanidanol-3 at 37 degrees C, significant increase of the haemolysis time could be demonstrated. The most expressive cytoprotective effect was observed at (+)-cyanidanol-3 concentrations of 2, 5 and 10 micrograms/ml. In vivo, two hours following the oral administration of 1.5 g Catergen, an increase of the haemolysis time for about 25% could be demonstrated. This simple method seems to be suitable for the investigation of the membrane stabilizing effect of (+)-cyanidanol-3.
D001578:Benzopyrans / Q000494:pharmacology*; D002392:Catechin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004910:Erythrocyte Membrane / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006851:Hydrochloric Acid / Q000633:toxicity
D001578:Benzopyrans; D002392:Catechin; D006851:Hydrochloric Acid