A micro-radioimmunoassay for apamin.
A radioimmunoassay is described which allows detection of quantities of apamin between 5 and 1200 fmoles (10-2400 pg) in 50 microliter aliquots. The assay requires a few milliliters of apamin antiserum diluted 10,000-20,000 times and 125I apamin at a specific radioactivity of 2000 Ci/mmole. This assay is specific for apamin.
D001030:Apamin / Q000032:analysis*; D001514:Bee Venoms / Q000032:analysis*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D007457:Iodine Radioisotopes; D008832:Microchemistry; D011863:Radioimmunoassay / Q000379:methods
D001514:Bee Venoms; D007457:Iodine Radioisotopes; D001030:Apamin