Connective tissue activating peptide III. Induction of synthesis and secretion of plasminogen activator by synovial fibroblasts.
Connective tissue activating peptide III (CTAP-III) is a platelet factor that induces, in cultured connective tissue cells, activities observed in chronic inflammation. In this study we measured plasminogen activator secretion by synovial fibroblasts after stimulation by CTAP-III. Increased plasminogen activator secretion was observed 24-48 hours after stimulation. Induction was prevented by dexamethasone (10(-9)-10(-7) M), cycloheximide (1 microgram/ml) and, variably, by actinomycin D (0.3 microgram/ml), but not by cytosine arabinoside (10(-4)M). This is the first evidence that CTAP-III induces degradative as well as proliferative activity by connective tissue cells.
D003513:Cycloheximide / Q000494:pharmacology; D003561:Cytarabine / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D010455:Peptides / Q000502:physiology*; D010960:Plasminogen Activators / Q000096:biosynthesis* / Q000378:metabolism; D013583:Synovial Membrane / Q000166:cytology*
D010455:Peptides; D003561:Cytarabine; D003609:Dactinomycin; C009862:connective tissue-activating peptide; D003907:Dexamethasone; D003513:Cycloheximide; D010960:Plasminogen Activators