title: Platelets and fibrin strands during clot retraction.
abstract: The ultrastructure of platelet fibrin contacts (PFC) and the course of the strands was investigated in serial sections of retracted clots with the help of specimen tilting. We found after retraction in a test tube as well as under isometric conditions in the resonance thrombograph, after HARTERT, an uniform type of PFC. The side to side contact between platelet surface and fibrin strands displayed a 15 nm wide space which was bridged of 10 - 30 nm by filamentary structure. In each case the direction of the fibrin strands changed on contact with the platelet surface (bend). These bends recurred if the adhering strands ran over a longer distance on the platelet surface. The bends can be explained by non-directional movement of the platelets or of their pseudopodia. Microfilaments (actomyosin) which run straight in pseudopodia and often also twisted in the platelet body support this assumption. The described mechanism - contact of the thrombin activated platelets with fibrin strands and simultaneous nondirectional movement of the platelets which bind further sections of the adhering strands to their surface - would provide a more satisfactory explanation for the retraction of the clot to 1/10 of its original volume.
mesh_terms: D001792:Blood Platelets / Q000502:physiology* / Q000648:ultrastructure; D003018:Clot Retraction; D003285:Contractile Proteins / Q000097:blood; D003599:Cytoskeleton / Q000502:physiology; D005337:Fibrin / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D008954:Models, Biological; D009068:Movement; D011554:Pseudopodia / Q000502:physiology
chemical_list: D003285:Contractile Proteins; D005337:Fibrin