High-performance liquid chromatographic method for the analysis of a candidate 8-aminoquinoline antileishmanial drug using oxidative electrochemical detection.
An analytical method was developed for the quantitation of a candidate antileishmanial drug, 6-methoxy-8-(6-diethylaminohexylamino)-4-methylquinoline, dihydrochloride, in canine plasma. The assay utilized internal standard technique with a structural similar 8-aminoquinoline, 6-methoxy-8-(7-diethylaminoheptylamino)-4-methylquinoline, dihydrochloride, as the internal standard. The method employs a liquid-solid extraction procedure with prepackaged silica gel columns upon which the drug and internal standard are adsorbed, then selectively washed and eluted. Reversed-phase chromatography was then employed to analyze the extracted sample by means of oxidative electrochemical detection at +0.75 V. Good accuracy and precision were obtained over the range of concentration tested (10-1500 ng/ml plasma). Analyses of plasma samples from human volunteers given the drug demonstrate the method is also suitable for analysis of human plasma samples. The entire procedure is relatively simple and requires only 1 ml of plasma.
D000634:Aminoquinolines / Q000097:blood* / Q000138:chemical synthesis; D000818:Animals; D000981:Antiprotozoal Agents / Q000097:blood* / Q000138:chemical synthesis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D004563:Electrochemistry; D006801:Humans; D007896:Leishmaniasis / Q000188:drug therapy; D010084:Oxidation-Reduction
D000634:Aminoquinolines; D000981:Antiprotozoal Agents