The kinetics of inhibition of human seminal plasma acid phosphatase by sodium fluoride.
Purified human seminal plasma acid phosphatase is inhibited by sodium fluoride and L(+) tartrate and 50% inhibitions (I50) have been obtained at 7.3 X 10(-5) and 3.0 X 10(-4) M concentrations, respectively. By Lineweaver-Burk plot, sodium fluoride shows non-competitive type of inhibition. Ackermann plot indicates that sodium fluoride is a reversible inhibitor of seminal plasma acid phosphatase. On polyacrylamide activity gel electrophoresis and by densitometric scan, a single molecular form of acid phosphatase has been identified in human seminal plasma. Inhibition patterns by sodium fluoride and L(+) tartrate have also been performed by polyacrylamide activity gel electrophoresis and almost complete inhibition of hydrolysis of alpha-napthol phosphate is observed at 20 mM and 100 mM of sodium fluoride and L(+) tartrate, respectively.
D000135:Acid Phosphatase / Q000037:antagonists & inhibitors*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007700:Kinetics; D008297:Male; D012661:Semen / Q000201:enzymology*; D012969:Sodium Fluoride / Q000494:pharmacology*
D012969:Sodium Fluoride; D000135:Acid Phosphatase