[Intraenterocytic metabolism and blood uptake of caproic and oleic acids in control and actidione-cycloheximide-treated rats].
Intestinal absorption of capric and oleic acids from the intestinal lumen into mesenteric portal vein blood was investigated in control and actidione-cycloheximide-treated rats using the ex vivo vascular perfusion technique. The measurement of 14C-labeled lipids, 14CO2 and 14C-labeled acid-soluble products was simultaneously carried out with blood collected at 5-min intervals for 60 min. The enterocyte catabolism of capric and oleic acids, one of which was preferentially absorbed via the blood, has been interpreted by the different processes of absorption. The greater enterocyte catabolic activity with capric acid, compared to oleic acid, could be related to the slight affinity of FABP Z for this medium-chain fatty acid and also to lack of capric acid esterification; a non-negligible fraction of this fatty acid actually disappeared from the lumen and was catabolized into the enterocyte at an early stage of its absorption. The actidione-cycloheximide treatment affected oleic acid absorption via the blood to a greater degree than that of capric acid. But it modified both intestinal blood absorption and the catabolism of the two fatty acids in the same manner.
D000818:Animals; D001345:Autoradiography; D001639:Bicarbonates / Q000097:blood; D003513:Cycloheximide / Q000494:pharmacology*; D003652:Decanoic Acids / Q000097:blood / Q000378:metabolism*; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism; D008055:Lipids / Q000097:blood; D008297:Male; D008854:Microscopy, Electron; D019301:Oleic Acid; D009829:Oleic Acids / Q000097:blood / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D001639:Bicarbonates; D003652:Decanoic Acids; D008055:Lipids; D009829:Oleic Acids; D019301:Oleic Acid; C031071:decanoic acid; D003513:Cycloheximide
Métabolisme intraentérocytaire et absorption par la voie sanguine des acides caprique et oléique chez le rat témoin et traité par l'actidione-cycloheximide.