Stimulation of RNA polymerases I and II from mouse L1210 leukemia and Ehrlich ascites carcinoma cells by spermine and spermidine and its modification by ammonium sulfate.
Nuclear RNA polymerases from murine L1210 leukemia and Ehrlich carcinoma cells were stimulated more effectively by spermine than by spermidine. Optimal stimulatory concentrations of spermine and spermidine for Ehrlich polymerases Ia and Ib decreased to physiological values and maximal stimulation increased as the concentration of (NH4)2SO4 was reduced from 0.08 to 0 M. In the presence of 0.062-0.074 M (NH4)2SO4 L1210 polymerases Ia, IIa and IIb were stimulated significantly by both polyamines, whereas, at (NH4)2SO4 concentrations of 0.11-0.17 M, stimulation was suppressed and high concentrations of the polyamines were inhibitory. Similarly, stimulation of Ehrlich solubilized polymerase by polyamines was inhibited by 0.064 M (NH4)2SO4.
D000645:Ammonium Sulfate / Q000494:pharmacology*; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D002467:Cell Nucleus / Q000201:enzymology; D004347:Drug Interactions; D007700:Kinetics; D007939:Leukemia L1210 / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology; D051379:Mice; D008815:Mice, Inbred Strains; D012318:RNA Polymerase I / Q000378:metabolism*; D012319:RNA Polymerase II / Q000378:metabolism*; D013095:Spermidine / Q000494:pharmacology*; D013096:Spermine / Q000494:pharmacology*
D013096:Spermine; D012319:RNA Polymerase II; D012318:RNA Polymerase I; D000645:Ammonium Sulfate; D013095:Spermidine