title: Characterization of low- and high-metastatic clones isolated from a Lewis lung carcinoma.
abstract: Cloned cell lines with low and high metastatic potentials were established from a Lewis lung carcinoma. Those with a high metastatic potential, LM12 -3-II cells, grew slower than those with a low potential, P-29 cells, both in vivo and in vitro. No significant difference between these cell lines was found in their susceptibility to natural killer cell-mediated lysis in vitro. Both LM12 -3-II and P-29 cells showed poor organization of microfilament bundles containing actin. LM-12-3-II cells showed lower cloning efficiency in semisolid 0.3% and 0.6% agar medium, lower lysosomal enzyme activities and lower homotypic aggregation than P-29 cells. LM12 -3-II cells adhered to monolayers of endothelial cells more slowly than P-29 cells, although they adhered to a subendothelial matrix a little more rapidly than P-29 cells. On the other hand, LM12 -3-II cells adhered to the surface of plastic culture dishes more firmly than P-29 cells. The neuraminidase-accessible sialic acid of LM12 -3-II cells was less than that of P-29 cells. Thus, the firmness of adhesion was positively correlated with the metastatic potential in these cells.
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002462:Cell Membrane / Q000032:analysis; D002999:Clone Cells; D003599:Cytoskeleton / Q000648:ultrastructure; D003602:Cytotoxicity, Immunologic; D006651:Histocytochemistry; D007694:Killer Cells, Natural / Q000276:immunology; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary* / Q000648:ultrastructure; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019158:N-Acetylneuraminic Acid; D009360:Neoplastic Cells, Circulating / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000166:cytology*; D012794:Sialic Acids / Q000032:analysis; D013234:Stem Cells / Q000166:cytology*
chemical_list: D000199:Actins; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid