title: Changes in cytoplasmic and lysosomal enzyme activities in cultured rat heart cells: the relationship to cell differentiation and cell population in culture.
abstract: Postnatal rat heart cells in culture enriched with respect to muscle cells were obtained by either high density seeding or by the replating technique. [3H]Thymidine incorporation to DNA and the enzymatic pattern of cytoplasmic and lysosomal enzymes have been studied as a function of the culture's age, of seeding density, and replating. It was shown that replating maintains predominance of myocyte population for at least 2 wk in culture; heavy seeding density allows homogeneous myocyte population for the 1st wk in culture; and the enzyme profile of the culture may serve as an indicator for the type of cell population in culture and its state of differentiation.
mesh_terms: D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation; D002478:Cells, Cultured; D003593:Cytoplasm / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D009206:Myocardium / Q000166:cytology / Q000201:enzymology*; D011506:Proteins / Q000032:analysis; D051381:Rats
chemical_list: D011506:Proteins