The effects of phenobarbital, bis-p-nitrophenyl phosphate and disulfiram on the hydrolysis of propanidid in Wistar rats.
The short-acting anesthetic propanidid was used in vivo as a model substrate in studies of phenobarbital-inducible carboxylesterases in Wistar rats. Phenobarbital shortened the duration of anesthesia produced by intravenous (25 mg/kg) or intraperitoneal (750 mg/kg) administration of propanidid which was in good agreement with the lowered plasma concentration and the shortened half-life of propanidid in phenobarbital-pretreated rats. The treatment of rats with bis-p-nitrophenyl phosphate or disulfiram with or without phenobarbital-pretreatment prolonged the sleeping time and the half-life of propanidid in plasma.
D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000201:enzymology; D004221:Disulfiram / Q000494:pharmacology*; D004285:Dogs; D006868:Hydrolysis; D008099:Liver / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D009596:Nitrophenols / Q000494:pharmacology*; D010634:Phenobarbital / Q000494:pharmacology*; D011410:Propanidid / Q000097:blood / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012890:Sleep / Q000187:drug effects; D013997:Time Factors
D009596:Nitrophenols; C002887:bis(4-nitrophenyl)phosphate; D011410:Propanidid; D002265:Carboxylic Ester Hydrolases; D004221:Disulfiram; D010634:Phenobarbital