Release of catecholamines and [Met5]enkephalin immunoreactive material from perfused bovine adrenal glands.
Retrogradely perfused bovine adrenal glands were stimulated by acetylcholine (ACh) and 1,1-dimethyl-4-phenyl-piperazinium (DMPP), with or without hexamethonium. Stimulation by either agent resulted in an increased release of both [Met5]enkephalin immunoreactive material (ME-IRM) and catecholamines as measured by radioimmunoassay and high performance liquid chromatography with electrochemical detection, respectively. ACh (5 X 10(-5) M) and DMPP (5 X 10(-5) M) stimulated the release of norepinephrine greater than the release of epinephrine. The action of these agents was antagonized by hexamethonium (5 X 10(-4) M). DMPP produced the same percentage increase in the release of ME-IRM and norepinephrine but produced a greater percentage increase in the release of ME-IRM compared to the release of epinephrine. There was no apparent correlation between the percentage increase in the release of ME-IRM and either norepinephrine or epinephrine after ACh.
D000109:Acetylcholine / Q000494:pharmacology; D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002395:Catecholamines / Q000378:metabolism*; D002417:Cattle; D004246:Dimethylphenylpiperazinium Iodide / Q000494:pharmacology; D004744:Enkephalin, Methionine / Q000276:immunology / Q000378:metabolism*; D066298:In Vitro Techniques; D010477:Perfusion
D000906:Antibodies; D002395:Catecholamines; D004246:Dimethylphenylpiperazinium Iodide; D004744:Enkephalin, Methionine; D000109:Acetylcholine