Serum antibodies against glycosphingolipids in chronic relapsing experimental allergic encephalomyelitis. Demonstration by ELISA and relation to serum in vivo demyelinating activity.
Sera from guinea pigs with spinal cord-induced chronic relapsing experimental allergic encephalomyelitis (crEAE) were tested for IgG antibodies against glycosphingolipids (GSL; galactocerebroside, ganglioside GM1, sulfatide) by an enzyme-linked immunosorbent assay and for in vivo demyelinating activity by infusion into the lumbosacral subarachnoid space of normal rats. In chronic stage-crEAE sera (40-200 days after sensitization) a high incidence (21/26) and high titers (up to 1:2560) of antibodies against one or more GSL coincided with a high incidence (22/26) of in vivo demyelinating activity. These results suggest an involvement of antibodies against various GSL in the process of demyelination.
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005677:G(M1) Ganglioside / Q000276:immunology; D005699:Galactosylceramides / Q000276:immunology; D006028:Glycosphingolipids / Q000276:immunology*; D006168:Guinea Pigs; D009185:Myelin Proteins / Q000276:immunology; D009186:Myelin Sheath / Q000276:immunology / Q000473:pathology*; D051381:Rats
D000906:Antibodies; D005699:Galactosylceramides; D006028:Glycosphingolipids; D009185:Myelin Proteins; C002454:galactocerebroside; D005677:G(M1) Ganglioside