Glucose production in the perfused dog liver: effect of free fatty acids and ketones.
The perfused rat liver model has been used widely for the past 30 years to investigate various aspects of hepatic metabolism. Certain aspects of the regulation of glucose production, however, are different in rats and in higher mammals. A method for perfusing dog livers in situ has been developed. It is easier to perform and to standardize than previously described techniques, and enables the direct effect of various substances on glucose production to be tested. This new approach has been used to assess the influence of ketones and albumin-bound free fatty acids (FFA) on total hepatic glucose output. In contrast to the response generally observed in the rat, it was found that an increased availability of FFA or ketones caused a net decrease in glucose output from the dog liver. Thus, perfusion of the dog liver is a feasible technique that is particularly useful because the responses in rats and dogs are not always the same.
D020155:3-Hydroxybutyric Acid; D000818:Animals; D004285:Dogs; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D005947:Glucose / Q000096:biosynthesis*; D006885:Hydroxybutyrates / Q000494:pharmacology; D066298:In Vitro Techniques; D007659:Ketones / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008722:Methods; D010477:Perfusion
D005230:Fatty Acids, Nonesterified; D006885:Hydroxybutyrates; D007659:Ketones; D005947:Glucose; D020155:3-Hydroxybutyric Acid