Structural study on liver gelactin.
Electron microscopy, ultracentrifugation, gel filtration, and isoelectric focusing were carried out with gelactin, an actin-gelling protein from rabbit liver. Gelactin is a dimeric acidic protein (isoelectric point (pI) = 5.45), with a molecular weight of 190 000, a Svedberg constant of 6.25, and a Stoke's radius and length of 7.0 and 28 nm, respectively. While different from alpha-actinin by pI and amino acid composition, gelactin belongs by its dimensions to the class of alpha-actinins.
D000818:Animals; D002352:Carrier Proteins; D002850:Chromatography, Gel; D007525:Isoelectric Focusing; D008099:Liver / Q000032:analysis*; D046911:Macromolecular Substances; D008840:Microfilament Proteins / Q000302:isolation & purification*; D008854:Microscopy, Electron; D008970:Molecular Weight; D011817:Rabbits; D014461:Ultracentrifugation
D002352:Carrier Proteins; C034037:F-actin-binding proteins; D046911:Macromolecular Substances; D008840:Microfilament Proteins