Preparation of 40S ribosomal subunit proteins of rat liver by combination of ion exchange chromatography with reversed phase liquid chromatography.
Total protein of small subunits of rat liver ribosomes was fractionated by chromatography on carboxymethylcellulose. Aliquots (between 5 and 30 mg) of the protein material of each peak were further separated by reversed phase liquid chromatography on an octadecasilyl silica column. In this way 15 of the 32 proteins of the small ribosomal subunit of rat liver could be isolated in milligram amounts.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000032:analysis*; D046911:Macromolecular Substances; D008970:Molecular Weight; D051381:Rats; D012269:Ribosomal Proteins / Q000302:isolation & purification*; D012270:Ribosomes / Q000032:analysis
D046911:Macromolecular Substances; D012269:Ribosomal Proteins