The Giemsa-11 technique for species-specific chromosome differentiation. A simple stain modification leading to dependable direct and sequential staining procedures.
Oxidizing Methylene Blue and adding the reaction products to Eosin Y and Azure B makes possible a highly reliable Giemsa-11 technique for discrimination of chromosomes in hybrid cells according to their parental origin. This staining can be combined in a sequential procedure with a fluorescent banding technique allowing the exact identification of the chromosomes.
D000818:Animals; D001399:Azure Stains; D002875:Chromosomes / Q000648:ultrastructure*; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology; D007621:Karyotyping; D008677:Metaphase; D051379:Mice; D013045:Species Specificity
D001399:Azure Stains