A stable isotope method for the quantification of N-acetyl-5-aminosalicylic acid in plasma and urine.
The synthesis of a number of N-acyl-5-aminosalicylic acids and their derivatives is described. These compounds allow the sensitive and specific mass spectrometric determination of unlabelled or deuterium-labelled N-acetyl-5-aminosalicylic acid in biological samples. An in vivo study shows that the labelled compound, administered to rats, is excreted isotopically unchanged to at least 97%, and that no significant deacetylation/acetylation mechanism exists for this metabolite N-acetyl-5-aminosalicylic acid of salicylazosulphapyridine.
D000636:Aminosalicylic Acids / Q000032:analysis* / Q000097:blood / Q000652:urine; D000818:Animals; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000636:Aminosalicylic Acids; C028836:N-acetyl-5-aminosalicylic acid; D003903:Deuterium