Effects of vitamin E in kid erythrocytes on Tween 20-induced hemolysis in vitro.
Red blood cells (RBC) of alpha-tocopherol sufficiency and deficiency were produced in kids fed on purified diets to investigate the relationship between RBC alpha-tocopherol levels and hemolysis induced by Tween 20. RBC alpha-tocopherol contents of vitamin E-deficient kids were non-detectable from 4 weeks, and percentages of hemolysis increased to over 70%. A highly significant decrease in hemolysis was noted when vitamin E was added to the diet. Tween 20 hemolysis values were negatively correlated with RBC tocopherol levels in kids. The concentration of RBC alpha-tocopherol required to prevent the hemolysis was found to be 0.6 microgram/ml packed cells.
D000818:Animals; D000831:Animals, Newborn; D004032:Diet; D004912:Erythrocytes / Q000378:metabolism*; D006041:Goats / Q000097:blood*; D006461:Hemolysis / Q000187:drug effects*; D066298:In Vitro Techniques; D010949:Plasma / Q000378:metabolism; D011136:Polysorbates / Q000494:pharmacology*; D014810:Vitamin E / Q000008:administration & dosage / Q000097:blood*; D014811:Vitamin E Deficiency / Q000097:blood / Q000662:veterinary
D011136:Polysorbates; D014810:Vitamin E