title: The characterization of invertebrate troponin C.
abstract: TNCs from lobster, mussel, and squid migrated with rabbit TNC at an apparent mol. wt of 18,000. Electrophoretic mobilities in the presence or absence of Ca2+ were compared: the electrophoretic mobility of rabbit TNC was greater in the presence of Ca2+ than it its absence, but all invertebrate TNCs tested migrated identically, whether Ca2+ was present or not. The Ca2+-binding capacity of invertebrate TNCs was only one Ca2+ ion per molecule. The alpha-helix contents in the presence or absence of Ca2+ were compared: rabbit TNC changed by a value of 16% and invertebrate TNCs by 4%. Antibodies to loligo TNC did not cross-react with rabbit TNC, but did interact with their molluscan TNCs.
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000032:analysis; D002118:Calcium / Q000378:metabolism; D002942:Circular Dichroism; D049832:Decapodiformes / Q000032:analysis*; D009132:Muscles / Q000032:analysis; D008121:Nephropidae / Q000032:analysis*; D011485:Protein Binding; D011487:Protein Conformation; D011817:Rabbits; D013045:Species Specificity; D014336:Troponin / Q000302:isolation & purification* / Q000378:metabolism; D019209:Troponin C
chemical_list: D014336:Troponin; D019209:Troponin C; D002118:Calcium