Manganese ions inhibit acetylcholine receptor synthesis in cultured mouse soleus muscles.
Thin bundles dissected from mouse soleus muscles were kept in culture media containing 10% calf serum. After 3 days of culture at 37 degrees C the muscles were tested for acetylcholine (ACh) sensitivity by measuring the force of the contracture produced by 10 microM ACh. The ACh-receptor density was measured by the [125I]alpha-bungarotoxin method. When the [Ca2+] of the culture medium was 0.02 mM (instead of the normal 2 mM), ACh sensitivity and ACh-receptor density were not different from control. Replacement of 99% Ca2+ by Sr2+ or by Mg2+ was also without effect. The muscles did not survive in solutions containing Ni ions. ACh sensitivity and ACh-receptor density were at levels characteristic of non-cultured muscles when the culture medium contained 0.4-1.8 mM Mn2+. Since the incorporation of ACh receptors into the membrane was not affected by Mn ions these results indicate that Mn ions inhibit the synthesis of ACh receptors.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D003864:Depression, Chemical; D005260:Female; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008345:Manganese / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D009121:Muscle Denervation; D009132:Muscles / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000096:biosynthesis*; D013324:Strontium / Q000494:pharmacology
D011950:Receptors, Cholinergic; D008345:Manganese; D008274:Magnesium; D000109:Acetylcholine; D013324:Strontium