Ultrastructural localization of lectin binding sites of the acrosomal membrane system of boar spermatozoa.
The distribution of the main lectin receptors of boar spermatozoa were analyzed by concanavalin A and Ricinus communis agglutinin-120 visualized for light microscopy by enzymatic amplification with 3-amino-9-ethylcarbazole. The lectin binding sites of the acrosomal membranes in addition to the plasma membrane were displayed by electron microscopy using the lectin-peroxidase and lectin-ferritin technique. Lectin binding was particularly prominent at the anterior part of the head. It could be shown that both acrosomal membranes contain receptor sites for concanavalin A and Ricinus communis-agglutinin-120. Additionally, the whole cell surface could be labeled with both lectins.
D000177:Acrosome / Q000648:ultrastructure*; D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D003208:Concanavalin A / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008297:Male; D037121:Plant Lectins; D011975:Receptors, Mitogen / Q000032:analysis*; D013094:Spermatozoa / Q000648:ultrastructure*; D013552:Swine
D037102:Lectins; D037121:Plant Lectins; D011975:Receptors, Mitogen; C017707:Ricinus communis agglutinin-1; D003208:Concanavalin A