Use of enzyme-gold complexes for the ultrastructural localization of hemicelluloses in the plant cell wall.
Enzyme-gold complexes have been prepared with an endo beta 1----4 xylanase (EC 3.2.1.8) and a beta 1----4 mannanase (EC 3.2.1.78). The complexes were applied to ultrathin sections of plant cell walls for the ultrastructural localization of xylans in different tissues of a graminea and for the localization of glucomannans in the tracheids of spruce wood. The method proved to be highly specific and gave a very good contrast of the substrate polysaccharides. Used in conjunction with other cytochemical staining the enzyme-gold labelling provided information about the relative distribution of pectic polymers and xylans in primary walls.
D002473:Cell Wall / Q000648:ultrastructure; D043364:Endo-1,4-beta Xylanases; D006026:Glycoside Hydrolases / Q000378:metabolism; D006046:Gold; D006651:Histocytochemistry; D008361:Mannosidases / Q000378:metabolism; D010944:Plants / Q000648:ultrastructure*; D011134:Polysaccharides / Q000032:analysis*
D011134:Polysaccharides; D006046:Gold; C007916:hemicellulose; D006026:Glycoside Hydrolases; D008361:Mannosidases; C041867:endo-1,4-beta-D-mannanase; D043364:Endo-1,4-beta Xylanases