Studies of phosphorus metabolism by isolated nuclei. XII. Some fundamental properties of the incorporation of 32Pi into polyphosphate by rat liver nuclei.
Rat liver nuclei incubated in vitro catalyze a sustained incorporation of 32Pi into polyphosphate. A preliminary estimate indicates a minimal rate of 10 moles of Pi incorporation into polyphosphates/h/mg protein. Polyphosphate is the predominant acid-insoluble product of nuclear phosphorylation; its formation is dependent on the presence of a divalent cation and is catalyzed by a system or systems as yet uncharacterized.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D011122:Polyphosphates / Q000378:metabolism*; D051381:Rats
D010710:Phosphates; D010761:Phosphorus Radioisotopes; D011122:Polyphosphates