A standardized 'in vivo' model for the study of experimental arterial thrombosis: description of a method.
A method for "in vivo" induction and registration of arterial platelet thrombosis has been developed and standardized in a branch of the mesenteric artery of the white Wistar rat. It consists in local deendothelialization by electrical current. Thrombus induction is performed by topical superfusion with ADP; when the superfusion is discontinued the thrombus disappears but can again be induced reproducibly by renewal of the ADP superfusion after a time interval not exceeding 15 minutes. Registration of the thrombotic phenomenon is made possible by microprojection of the investigated arterial segment. An appropriate optoelectronic device allows the on-line derivation of discriminating parameters. Off-line storage and processing of the experimental data by computer is provided. The method is automatized and can easily be applied to other species of laboratory animals.
D000244:Adenosine Diphosphate; D000818:Animals; D003201:Computers; D004195:Disease Models, Animal; D004556:Electric Injuries; D008297:Male; D008638:Mesenteric Arteries; D008641:Mesenteric Vascular Occlusion / Q000209:etiology; D051381:Rats; D011919:Rats, Inbred Strains; D013927:Thrombosis / Q000209:etiology*
D000244:Adenosine Diphosphate