Separation of brain monosialoganglioside molecular species by high-performance liquid chromatography.
A method for the separation of molecular species of brain monosialogangliosides by high-performance liquid chromatography is described. GM4, GM3, GM2, and GM1 were purified from human brain and their individual molecular species were separated on a C18 reversed-phase column. Peaks were identified by mass spectrometry of the intact ganglioside, by gas-liquid chromatography of the fatty acids, and by high-performance liquid chromatography of the long chain bases. A characteristic elution sequence of molecular species permitted their identification based upon their retention times on the reversed-phase column.
D001923:Brain Chemistry; D002518:Ceramides / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005227:Fatty Acids / Q000032:analysis; D005677:G(M1) Ganglioside / Q000302:isolation & purification; D005678:G(M2) Ganglioside / Q000302:isolation & purification; D005679:G(M3) Ganglioside / Q000302:isolation & purification; D005732:Gangliosides / Q000302:isolation & purification*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods
D002518:Ceramides; D005227:Fatty Acids; D005679:G(M3) Ganglioside; D005732:Gangliosides; D005678:G(M2) Ganglioside; D005677:G(M1) Ganglioside; C028253:ganglioside, GM4