title: Allergens of Schistosoma mansoni. II. Fractionation and characterization of S. mansoni egg allergens.
abstract: The interaction of Schistosoma mansoni crude soluble egg antigen (SEA) with IgE antibodies in sera from S. mansoni-infected mice, rats and humans has been studied by the radioallergosorbent test (RAST) and the Prausnitz-Küstner (PK) technique. IgE antibodies recognizing egg antigens were present as early as day 21 after the infection in the mouse sera and day 28 in rat sera. IgE in sera of infected humans reacted with antigenic components in the Mr range 70,000-150,000 and focusing as a broad peak in the pH range 4.5-6.5 as measured by RAST. SDS-PAGE followed by western blotting showed the presence of major components at molecular weights of 117,000 and 35,000-43,000. In the PK test, using mouse sera, components focusing in the alkaline pH range also gave a positive reaction. Most of the allergenic activity was bound by concanavalin A-Sepharose and by wheat germ agglutinin-Ultrogel. IgE in serum from an infected non-permissive host (the Fischer rat) apparently recognized egg-stage-specific allergen as indicated by differences in the time course of the IgE response to egg allergens compared to the adult material. When analyzed by SDS-PAGE and western blotting with day 45-infected rat serum, SEA showed some qualitative and quantitative differences to adult worm antigen. Molecules at molecular weights between 25,000 and 30,000 and at about 43,000 in SEA reacted with rat serum IgE and were absent from adult worm antigen. The allergenic similarities between egg and adult worm are discussed.
mesh_terms: D000485:Allergens / Q000032:analysis / Q000302:isolation & purification*; D000818:Animals; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007428:Intradermal Tests; D007525:Isoelectric Focusing; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008810:Mice, Inbred C57BL; D010063:Ovum / Q000276:immunology; D011852:Radioallergosorbent Test; D051381:Rats; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains; D011935:Reagins / Q000096:biosynthesis; D012550:Schistosoma mansoni / Q000254:growth & development / Q000276:immunology*; D012552:Schistosomiasis / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology
chemical_list: D000485:Allergens; D011935:Reagins