title: Purification of synthetic oligodeoxyribonucleotides by ion-exchange high-performance liquid chromatography.
abstract: Synthetic oligodeoxyribonucleotides ranging from 11 to 37 nucleotides in length and with varying base compositions, prepared by both the phosphotriester and phosphite procedures, have been purified by ion-exchange high-performance liquid chromatography on Whatman Partisil 10/SAX columns using phosphate buffer gradients. The effects of different buffer systems on elution times and resolution have been evaluated. Oligomer composition and length had a marked effect on the resolution achieved. In general the use of formamide buffers gave the best results, particularly in the case of 2'-deoxyguanosine-rich sequences. These methods have also been successfully applied to the purification of mixtures of synthetic oligodeoxynucleotides.
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange; D009838:Oligodeoxyribonucleotides / Q000032:analysis*; D009841:Oligonucleotides / Q000032:analysis*
chemical_list: D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides