Protein conformation and reversed-phase high-performance liquid chromatography.
The structure of a series of proteins has been investigated by circular dichroism, fluorescence and visible spectroscopy as well as by differential scanning calorimetry under reversed-phase high-performance liquid chromatography elution conditions. These studies show that 1-propanol, a typical eluent, induces a reversible conformational change in proteins to an apparently ordered, helical form. This structural transition occurs in the range of propanol concentrations that produces elution of a particular protein. The possible relationship between this conformational change and protein elution is considered.
D000433:1-Propanol; D002152:Calorimetry, Differential Scanning; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011487:Protein Conformation; D012997:Solvents; D013050:Spectrometry, Fluorescence
D012997:Solvents; D000433:1-Propanol