Modulation of early mRNA synthesis in lectin-induced lymphocyte activation--an association with a possible factor.
Two phases of early mRNA synthesis were observed in lectin-induced lymphocyte activation. At 0-2 h, lectin-treated lymphocytes showed a considerable decrease in nuclear RNA polymerase II activity as compared with that of untreated cells and, at later periods, a reverse increase was observed. Such modulation of mRNA synthesis is not due to the change of RNA polymerase II, but a possible factor in nuclei. This factor represses RNA chain initiation and stimulates chromatin-dependent RNA elongation catalyzed by RNA polymerase II. It was characterized to be a 10-30 kDa sugar-containing molecule and the physiological significance of this factor is discussed from the viewpoint of the transcriptional regulation.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D012319:RNA Polymerase II / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*
D002843:Chromatin; D037102:Lectins; D012333:RNA, Messenger; D012319:RNA Polymerase II