[Lactate dehydrogenase activity and its isoenzyme in a system coupled with bacterial luciferase].
Properties of a coupled system "LDH-bacterial luciferase" were studied. The light-generating enzymatic system from luminescent bacteria Photobacterium fisheri was used as an indicator of the dehydrogenase activity. Kinetic parameters of the coupled system were studied using commercially available preparation of LDH and the enzyme from human blood plasma. The luminescent activity of bacterial preparation was shown to correlate with the activity of LDH and its isoenzymes within wide range of blood plasma concentrations.
D006801:Humans; D007527:Isoenzymes; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D008156:Luciferases / Q000097:blood*; D008163:Luminescent Measurements; D009243:NAD / Q000097:blood; D010776:Photobacterium / Q000201:enzymology
D007527:Isoenzymes; D009243:NAD; D007770:L-Lactate Dehydrogenase; D008156:Luciferases
Issledovanie aktivnosti laktatdegidrogenazy i ee izofermentov v sopriazhennoĭ sisteme s bakterial'noĭ liutsiferazoĭ.