[Isolation and quantitative determination of apoprotein C-11 in the blood using rocket immunoelectrophoresis].
Apoprotein C-II was isolated from human blood plasma by means of column chromatography-gel filtration and DEAE ion exchange chromatography by modified method of Brown et al (1969). Production of antiserum to apoprotein C-II as well as a procedure for rocket immunoelectrophoresis are described for estimation of the apoprotein in blood plasma of men and women of various age, exhibiting different lipid spectra. Content of the apoprotein C-II in blood plasma, estimated by the immunoelectrophoretic procedure, was higher in men as compared with women and correlated with concentration of triglycerides in blood plasma.
D000328:Adult; D053304:Apolipoprotein C-II; D001056:Apolipoproteins C / Q000097:blood / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male; D008875:Middle Aged; D012737:Sex Factors; D014280:Triglycerides / Q000097:blood
D053304:Apolipoprotein C-II; D001056:Apolipoproteins C; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D014280:Triglycerides
Vydelenie apoproteina C-11 i kolichestvennoe opredelenie ego v plazme krovi metodom raketnogo immunoélektroforeza.