Comparative study of the effects of various cryoprotectants in preserving the morphology of frozen and thawed fowl spermatozoa.
The effects of various cryoprotectants (glycerol, dimethylsulphoxide (DMSO), methylformamide and ethylene glycol) of the same molarity on preserving the morphology of frozen and thawed fowl spermatozoa (especially midpiece and acrosome) were examined under the light (LM) and scanning electron microscope (SEM) to determine the most suitable. Under LM, the mean respective increases in sperm deformity in semen diluted with ethylene glycol, methylformamide, glycerol and DMSO were 4.5, 4.9, 5.0 and 6.5 percentage points. Under SEM, the mean respective increases in acrosomal deterioration in semen diluted with glycerol, ethylene glycol, DMSO and methylformamide were 6.6, 8.8, 13.1 and 31.2 percentage points. From these results, it appears that glycerol is superior to ethylene glycol DMSO and methylformamide as a cryoprotectant.
D000177:Acrosome / Q000648:ultrastructure; D000818:Animals; D002645:Chickens / Q000502:physiology*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D019855:Ethylene Glycol; D005026:Ethylene Glycols / Q000494:pharmacology; D005559:Formamides / Q000494:pharmacology; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology; D008297:Male; D008855:Microscopy, Electron, Scanning; D012662:Semen Preservation / Q000662:veterinary*; D013094:Spermatozoa / Q000002:abnormalities / Q000187:drug effects* / Q000648:ultrastructure
D003451:Cryoprotective Agents; D005026:Ethylene Glycols; D005559:Formamides; D019855:Ethylene Glycol; D005990:Glycerol; C002950:methylformamide; D004121:Dimethyl Sulfoxide