Composition of O-antigenic lipopolysaccharides from Enterobacter cloacae.
Analyses have been carried out on lipopolysaccharides (LPS) from 14 strains of Enterobacter cloacae representing different O serotypes. All of the products appeared to have a composition and architecture typical of enterobacterial LPS, but points of interest include the absence of phosphate residues from the core oligosaccharide, the presence of both L-glycero-D-mannoheptose and D-glycero-D-mannoheptose (ratio usually about 4:1), and the presence in lipid A of small amounts of fatty acids with odd numbers of carbon atoms (mainly C13) in addition to tetradecanoic acid and 3-hydroxytetradecanoic acid. Monosaccharides identified as components of polymeric fractions from the LPS were glucose, galactose, mannose, rhamnose, glucosamine, galactosamine, fucosamine, and galacturonic acid. Most polymeric fractions also probably contained an O-acetyl substituent. Closely similar chemotypes found for the polymeric fractions from the LPS of cross-reacting serotypes support the view that these fractions contain the O-antigenic determinants and represent the side chains of the LPS.
D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002241:Carbohydrates / Q000032:analysis; D004754:Enterobacter / Q000032:analysis / Q000276:immunology*; D004755:Enterobacteriaceae / Q000276:immunology*; D005227:Fatty Acids / Q000032:analysis; D008070:Lipopolysaccharides / Q000276:immunology* / Q000302:isolation & purification; D009844:Oligosaccharides / Q000302:isolation & purification
D000942:Antigens, Bacterial; D002241:Carbohydrates; D005227:Fatty Acids; D008070:Lipopolysaccharides; D009844:Oligosaccharides