Effect of the gel-liquid crystalline phase transition on the reduction of cytochrome P-450 reconstituted into dimyristoyl-phosphatidylcholine liposomes.
The isolated purified components of the hepatic endoplasmic monooxygenatic system were incorporated into liposomes (dimyristoyl-phosphatidylcholine) by gel filtration technique. The temperature dependence of the reduction reaction in the range from 11 degrees C to 37 degrees C revealed a break in the Arrhenius-diagram at the phase transition temperature of dimyristoylphosphatidylcholine. The break point is in agreement with thermodynamic parameters derived from microcalorimetric studies of the monooxygenatic system reconstituted in the same way.
D000818:Animals; D003460:Crystallization; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004134:Dimyristoylphosphatidylcholine; D004227:Dithionite / Q000494:pharmacology; D005782:Gels; D008081:Liposomes; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D009249:NADP / Q000494:pharmacology; D010084:Oxidation-Reduction; D011817:Rabbits; D013696:Temperature
D005782:Gels; D008081:Liposomes; D004227:Dithionite; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D004134:Dimyristoylphosphatidylcholine