2',5'-Oligoadenylate and 2',5'-oligoadenylate phosphodiesterase in human plasma.
2',5'-Oligoadenylate and 2',5'-oligoadenylate phosphodiesterase activity were detected in the human plasma and serum by sensitive radioimmuno assays. The phosphodiesterase in the serum degraded 20 nM of added 2',5'-oligoadenylate in less than 1 hr. Addition of EDTA in the blood sample inhibited the phosphodiesterase activity completely and allowed the measurement of low levels of 2',5'-oligoadenylate. The concentration in the plasma from healty people was in the range of 0.03 to 0.3 nM.
D015088:2',5'-Oligoadenylate Synthetase / Q000097:blood*; D000227:Adenine Nucleotides / Q000097:blood*; D006801:Humans; D008832:Microchemistry / Q000379:methods; D009841:Oligonucleotides / Q000097:blood*; D009843:Oligoribonucleotides / Q000097:blood*; D011863:Radioimmunoassay; D012016:Reference Values
D000227:Adenine Nucleotides; D009841:Oligonucleotides; D009843:Oligoribonucleotides; C023505:2',5'-oligoadenylate; D015088:2',5'-Oligoadenylate Synthetase