[Type II glucocorticoid receptors in tissues of adrenalectomized rats].
In experiments on adrenalectomized Wistar male rats, weighing 100 to 110 g, the content of type II glucocorticoid receptors in the different tissues was studied. Dissociation constants and the number of binding sites were determined by Sketchard's method, using labelled triamcinolone acetonide with a high specific activity. It was shown that the maximum level (279 fmole/mg protein) of type II glucocorticoid receptors was found in hepatic cytosol and the minimum one (44 fmole/mg protein) in pulmonary cytosol. Dissociation constants of the glucocorticoid receptor-hormone complex in cytosol of the different tissues did not markedly differ (0.77 X 10(-8) M to 1.1 X 10(-8) M).
D000315:Adrenalectomy; D000818:Animals; D001665:Binding Sites; D003600:Cytosol / Q000032:analysis; D066298:In Vitro Techniques; D007668:Kidney / Q000648:ultrastructure; D008099:Liver / Q000648:ultrastructure; D008168:Lung / Q000648:ultrastructure; D008297:Male; D009206:Myocardium / Q000648:ultrastructure; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000032:analysis*; D011987:Receptors, Steroid / Q000032:analysis*; D014222:Triamcinolone Acetonide / Q000378:metabolism*; D014316:Tritium
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D014316:Tritium; D014222:Triamcinolone Acetonide
Sostoianie gliukokortikoidov retseptorov tipa II v tkaniakh adrenalektomirovannykh krys.