Lysosomal acid deoxyribonuclease from vervet monkey livers--II. Enzymic properties.
Primate liver lysosomal acid DNase is an endonucleolytic enzyme. The enzyme has both 3'- and 5'-nucleotidohydrolase activities. The oligonucleotides produced by DNase are polymers mainly about 30 mononucleotides long. The Arrhenius plot shows a discontinuity with a transition temperature at 47 degrees C, with an activation energy of 107 kJ/mol below and 67 kJ/mol above this temperature. The activation enthalpy is 104 kJ/mol and the entropy -0.498 kJ/mol/K. The enzyme is subject to substrate inhibition and the Km value is 159 X 10(-3) mM DNA-P.
D000818:Animals; D002151:Calorimetry; D002417:Cattle; D002521:Cercopithecus; D004247:DNA; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D013379:Substrate Specificity; D013816:Thermodynamics; D013950:Thymus Gland
D009838:Oligodeoxyribonucleotides; D004247:DNA; D004706:Endodeoxyribonucleases; C019329:deoxyribonuclease II