Preparation of RPC-5 like resin for HPLC (Neosorb LC) and its use for the separation of oligonucleotides and mononucleotides.
HPLC packing (Neosorb LC), which is an improved alternative resin of RPC-5, was prepared from fine granular Neosorb ND (polychlorotrifuluoroethylene powder) and Adogen 464. Various mononucleotides and oligonucleotides were separated very well by HPLC with this packing depending on the chain length, internucleotidic linkage, base composition, base sequence, and position and number of terminal phosphate residue.
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003854:Deoxyribonucleotides / Q000302:isolation & purification*; D009838:Oligodeoxyribonucleotides / Q000302:isolation & purification*; D009841:Oligonucleotides / Q000302:isolation & purification*; D009843:Oligoribonucleotides / Q000302:isolation & purification*; D011095:Polyethylenes; D012117:Resins, Synthetic; D012265:Ribonucleotides / Q000302:isolation & purification*; D013329:Structure-Activity Relationship
D003854:Deoxyribonucleotides; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D011095:Polyethylenes; D012117:Resins, Synthetic; D012265:Ribonucleotides; C039184:polychlorotrifluoroethylene