An enzyme-linked immunosorbent assay that measures protective antibody levels to Newcastle disease virus in chickens.
An enzyme-linked immunosorbent assay (ELISA) was developed to measure antibodies to Newcastle disease virus (NDV) in chickens. Chickens 6 to 33 weeks old, with a variety of vaccination histories, were bled before challenge with a velogenic strain of NDV. Fourteen days post-challenge, 63 of the 73 challenged birds had survived. ELISA results of pre-challenge sera corresponded directly with survival rates of birds challenged with NDV.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D009521:Newcastle Disease / Q000276:immunology* / Q000401:mortality; D009522:Newcastle disease virus / Q000276:immunology*
D000914:Antibodies, Viral