[Proteases from the enterocytes of the porcine small intestine. Role of aminopeptidase N in the transport of dipeptides].
The kinetics of radioactive label uptake from [U-14C]Gly, L-[4.5-3H]Leu and dipeptide [U-14C]Gly-L-[4.5-3H]Leu by the brush border membrane vesicles of porcine small intestine have been studied. The effect of aminopeptidase N inhibitors and of leucine-binding protein on the accumulation rates has been investigated. A comparison of the kinetic parameters for the uptake and hydrolysis of Gly-L-Leu, has demonstrated that the dipeptide transfer includes two conjugated steps, i. e., hydrolysis catalyzed by aminopeptidase N and transport of the resultant free amino acids by a specific carrier.
D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active; D004151:Dipeptides / Q000378:metabolism*; D006868:Hydrolysis; D007421:Intestine, Small / Q000166:cytology / Q000201:enzymology*; D007700:Kinetics; D008871:Microvilli / Q000201:enzymology; D013552:Swine
D004151:Dipeptides; C015905:glycylleucine; D000626:Aminopeptidases
Proteazy énterotsitov tonkogo kishechnika svin'i. Rol' aminopeptidazy N v transporte dipeptidov.