Evaluation of the enzymatic assay of serum uric acid with 2,2'-azino-di(3-ethylbenzthiazoline-6-sulphonate) (ABTS) as chromogen.
A spectrophotometric method for the determination of serum uric acid based on the oxidation of 2,2'-azino-di(3-ethylbenzthiazoline-6-sulphonate) by use of uricase and peroxidase has already been reported. The method is very precise (CV less than 4.7%). The standard curve is linear up to 4640 mumol/L. Comparison with other enzymatic methods gave good correlation. The method gave results 9% lower than the phosphotungstate method. The effects of bilirubin, haemoglobin, glucose, ascorbic acid, anticoagulants and purine compounds were studied. The reference values for this method are 140.8-407.8 mumol/L for female subjects and 145.6-514.7 mumol/L for male subjects.
D000925:Anticoagulants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000097:blood; D052160:Benzothiazoles; D001663:Bilirubin / Q000097:blood; D001786:Blood Glucose / Q000032:analysis; D006461:Hemolysis; D006801:Humans; D007202:Indicators and Reagents; D011687:Purines / Q000494:pharmacology; D012016:Reference Values; D013451:Sulfonic Acids; D014527:Uric Acid / Q000097:blood*
D000925:Anticoagulants; D052160:Benzothiazoles; D001786:Blood Glucose; D007202:Indicators and Reagents; D011687:Purines; D013451:Sulfonic Acids; D014527:Uric Acid; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D001205:Ascorbic Acid; D001663:Bilirubin