title: Interaction of choline with nicotinic and muscarinic cholinergic receptors in the rat brain in vitro.
abstract: The ability of choline to interact with nicotinic receptors was investigated by measuring its ability to inhibit the specific binding of [3H]-nicotine in rat brain. Choline, with an IC50 of 241 mumol/l, was three times more potent than its analogue deanol and almost 1000-fold less potent than acetylcholine. Choline also inhibited the binding of the antagonist [3H]-quinuclidinyl benzilate (IC50 = 2.5 mmol/l) and of the agonist [3H]-oxotremorine-M (IC50 = 165 mumol/l) to muscarinic cholinergic receptors. These results indicate that choline is able to interact directly, in vitro with brain cholinergic receptors of both the nicotinic and muscarinic type.
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D001923:Brain Chemistry / Q000187:drug effects*; D002794:Choline / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D011813:Quinuclidinyl Benzilate; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000187:drug effects*; D011978:Receptors, Nicotinic / Q000187:drug effects*
chemical_list: D011976:Receptors, Muscarinic; D011978:Receptors, Nicotinic; D011813:Quinuclidinyl Benzilate; D000110:Acetylcholinesterase; D002794:Choline