A rapid preparative method for isolation of neutral and acidic glycosphingolipids by radial thin-layer chromatography.
An efficient method to separate neutral and acidic glycosphingolipids (GSLs) from their mixtures within a short period (45-60 min) and with low consumption of solvents (chloroform-methanol-water, 60/35/8 (v/v/v); 250-500 ml) has been developed. This method utilizes a centrifugal thin-layer chromatograph (Chromatotron) and the GSL mixtures (30-400 mg) are applied to glass plates coated with a 1-mm layer of silica gel 60 PF-254. The method (radial thin-layer chromatography) is rapid and simple and the recovery of glycosphingolipids is high (70-80%).
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer / Q000379:methods; D004159:Diphenylamine; D004912:Erythrocytes / Q000032:analysis; D006028:Glycosphingolipids / Q000097:blood / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D011817:Rabbits; D012997:Solvents; D013552:Swine
D006028:Glycosphingolipids; D012997:Solvents; D004159:Diphenylamine