[Effect of different acclimatization temperatures on the ultrastructure of neuronal synapses of perch (Tilapia mariae; Cichlidae, Teleostei)].
The ultrastructure of synapses in the stratum fibrosum marginale (SM) of the optic tectum of cichlid fishes (Tilapia mariae) was investigated following acclimation to extreme environmental temperatures (experimental temperatures 16 degrees C and 32 degrees C; preference temperature: 26 degrees C). Four different types of synapses can be differentiated, from which 90% of all synapses belong to axodendritic spine synapses. The general ultrastructural appearance is more distinct in case of cold -adapted fishes, which in particular is due to a more pronounced staining of the polysaccharide-surface coat of the membranes. Analyzing quantitatively the axo-dendritic spine synapses (600 of each collective) significant differences were found for the length of the synaptic contact zone (16 degrees C: 0,391 micron; 32 degrees C: 0,369 micron) and for the thickness of postsynaptic densities (16 degrees C 42,6 nm; 32 degrees C: 52,7 nm). The number of mitochondria per presynapse was rised for cold-adapted animals to a value of 0,29 as compared to 0,18 mitochondria/presynapse for warm-adapted animals. The vesicle density per micron 2 of synaptic area, however, remained unchanged (approximately equal to 89 vesicles/micron 2). The morphological synaptic plasticity thus demonstrated in the optic tectum of chichlid teleosts, is correlated with behavioural changes and with alterations in the biochemical composition of synaptic membranes.
D000064:Acclimatization; D000818:Animals; D001369:Axons / Q000648:ultrastructure; D001833:Body Temperature Regulation; D003712:Dendrites / Q000648:ultrastructure; D005399:Fishes / Q000033:anatomy & histology*; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D009473:Neuronal Plasticity; D013477:Superior Colliculi / Q000033:anatomy & histology*; D013569:Synapses / Q000648:ultrastructure*; D013570:Synaptic Membranes / Q000648:ultrastructure; D013572:Synaptic Vesicles / Q000648:ultrastructure
Einfluss unterschiedlicher Akklimationstemperaturen auf die Ultrastruktur neuronaler Synapsen von Buntbarschen (Tilapia mariae; Cichlidae, Teleostei).