title: Calmodulin in starfish oocytes. II. Trypsin treatment suppresses the trifluoperazine-sensitive step.
abstract: The 1-methyladenine-induced oocyte maturation in starfish is reversibly inhibited by the anticalmodulin drug, trifluoperazine (TFP). However, when oocytes are exposed for 10 min to trypsin, they lose their sensitivity to TFP. Trypsin does not alter the length of the hormone-dependent period (1-methyladenine minimal contact time) or the 1-methyladenine concentration requirements. Trypsin-treated oocytes remain sensitive to other maturation inhibitors such as procaine, theophylline, caffeine, and D-600. Trypsin exposure modifies the protein pattern composition of the oocyte cortex (breakdown of a 140-kDa protein). TFP binding site localization was studied using fluorescence microscopy: in addition to a general diffuse fluorescence, staining is localized to probably acidic granules located in the cortex. Results are discussed in relation to calmodulin and plasma membrane calmodulin-dependent enzyme involvement in the stimulation of starfish oocyte maturation.
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002147:Calmodulin / Q000502:physiology*; D005260:Female; D008540:Meiosis / Q000187:drug effects; D008565:Membrane Proteins / Q000502:physiology; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D010740:Phospholipases / Q000502:physiology; D013215:Starfish / Q000502:physiology*; D014268:Trifluoperazine / Q000378:metabolism / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
chemical_list: D002147:Calmodulin; D008565:Membrane Proteins; D014268:Trifluoperazine; C008407:1-methyladenine; D010740:Phospholipases; D014357:Trypsin; D000225:Adenine