High tyrosine protein kinase activities in soluble and particulate fractions in bone marrow cells.
High tyrosine protein kinase activities were detected in soluble and particulate forms from bone marrow cells using synthetic peptide (Glu-Asp-Ala-Glu-Tyr-Ala-Ala-Arg-Arg-Arg-Gly) as a substrate. Total activity of tyrosine protein kinase was 2.4 times higher in the soluble fraction but the specific activity was slightly higher in the particulate one. Mg2+ or Mn2+ requirements of these two enzymes for maximal activity were quite different from each other. Physiological significance of these two forms of enzymes is briefly discussed.
D000818:Animals; D001853:Bone Marrow / Q000201:enzymology*; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D008297:Male; D008345:Manganese / Q000378:metabolism; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D011494:Protein Kinases / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D011092:Polyethylene Glycols; D008345:Manganese; D017830:Octoxynol; C010615:Nonidet P-40; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; D008274:Magnesium