Non-radiochemical procedure for the measurement of O-methylation of the stereoisomers of isoprenaline.
A non-radiochemical procedure has been developed which permits the separation and measurement of isoprenaline (ISO) and the O-methylated metabolite, 3-methoxyisoprenaline (MeOISO). This methodology employs alumina chromatography and toluene solvent extraction to separate the catecholamine, ISO, from the O-methylated derivative, MeOISO. High-performance liquid chromatography with electrochemical detection has been used to quantify these compounds. The biological application of this procedure includes the evaluation of O-methylation of the stereoisomers of ISO by intact tissues.
D000818:Animals; D001013:Aorta, Thoracic / Q000032:analysis; D065098:Catechol O-Methyltransferase Inhibitors; D002396:Catechols / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000032:analysis*; D008297:Male; D008745:Methylation; D011427:Propiophenones / Q000494:pharmacology; D011817:Rabbits; D013237:Stereoisomerism
D065098:Catechol O-Methyltransferase Inhibitors; D002396:Catechols; D011427:Propiophenones; C006104:U 0521; D007545:Isoproterenol