title: Chemical studies of partially hydrolysed lipopolysaccharides from four strains of Campylobacter jejuni and two strains of Campylobacter coli.
abstract: Lipopolysaccharides (LPS) from four strains of Campylobacter jejuni and two strains of C. coli were partially hydrolysed with 1% acetic acid. Subsequent chloroform extraction led to the formation of a polysaccharide-containing aqueous layer, an interfacial material and a lipid A-containing chloroform layer. The polysaccharides contained the neutral sugars, amino sugars, 2-keto-3-deoxy-octonic acid, and part of the phosphorus present in the undegraded LPS. The lipid As were made up of glucosamine, phosphorus, ester- and amide-linked 3-hydroxytetradecanoic acid, and ester-linked n-tetradecanoic and n-hexadecanoic acid. The interfacial material was made up of lipid A and undegraded LPS. When chromatographed on Bio-GEl P-60, the degraded polysaccharides were eluted as two incompletely separated peaks (strains NCTC 11168, NCTC 11351, 11041 and 11101) or as one peak (strains NCTC 11392 and E 8035). All peaks appeared close to the total volume of the column. When the different fractions were re-chromatographed on Bio-GEl P-10, the peaks still appeared close to the total volume of the column. These findings indicate that LPS from C. jejuni and C. coli are devoid of long O-antigenic side-chains.
mesh_terms: D002167:Campylobacter / Q000032:analysis*; D002168:Campylobacter fetus / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D006868:Hydrolysis; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial
chemical_list: D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial