Presence of fibroblast-transforming genes in normal DNA of several mouse and rat strains.
Cellular DNA of the inbred mouse strains BALB/c, C3Hf, C57BL, CBA, DBAf , GRS and ND2 and the inbred rat strains BN, SD and WAG was shown to oncogenically transform murine fibroblasts of the continuous cell line BALB/3T3. Subcutaneous inoculation of transformed cells into BALB/c mice led to the rapid development of sarcomas. For transformation the DNA had to be fragmented to a size smaller than 23 kilobase pairs (kbp), with a lower limit of 6.5 kbp. No transforming virus could be rescued from the transformed lines by infection with a murine leukemia virus.
D000818:Animals; D002471:Cell Transformation, Neoplastic; D004247:DNA / Q000235:genetics*; D005260:Female; D005347:Fibroblasts; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009857:Oncogenes; D051381:Rats; D011919:Rats, Inbred Strains; D012509:Sarcoma / Q000235:genetics; D014170:Transformation, Genetic
D004247:DNA