Oxidative degradation of thymine with O2 promoted by L-ascorbic acid and Cu(II) ion.
Oxidation of thymine with O2 was promoted by copper(I) ion generated from reaction of L-ascorbic acid (AA) with copper (II) ion. The main oxidation products were thymine glycol (TG) and N-formyl-N'-pyruvylurea (FPU). At higher concentration of O2, formation of FPU was favored, while TG was dominant at higher Cu(II) ion and lower O2 concentrations. Reaction mechanism involving hydroxy thyminyl radical was proposed.
D001205:Ascorbic Acid; D055598:Chemical Phenomena; D002621:Chemistry; D003300:Copper; D004355:Drug Stability; D007202:Indicators and Reagents; D010084:Oxidation-Reduction; D010100:Oxygen; D013941:Thymine
D007202:Indicators and Reagents; D003300:Copper; D001205:Ascorbic Acid; D013941:Thymine; D010100:Oxygen