Isolation and purification of bovine immunoglobulins: use of Sephacryl S-300 filtration avoids protein precipitation steps.
A simplified purification method for bovine IgG1, IgG2, IgM, IgA and S-IgA is presented. It takes advantage of Sephacryl S-300 gel filtration. When applied to this column, colostral whey can be separated into IgM, S-IgA and IgG rich fractions. Filtration of blood serum leads to IgM, IgA and IgG rich fractions. IgM, IgG1, IgG2, S-IgA and IgA are then further purified by anion exchange chromatography.
D000180:Acrylic Resins; D000818:Animals; D002417:Cattle / Q000276:immunology*; D002850:Chromatography, Gel; D003126:Colostrum / Q000032:analysis; D007070:Immunoglobulin A / Q000302:isolation & purification*; D007075:Immunoglobulin M / Q000302:isolation & purification*; D011233:Precipitin Tests
D000180:Acrylic Resins; D007070:Immunoglobulin A; D007075:Immunoglobulin M; C037239:Sephacryl Superfine