[Changes in the state of hormone-receptor complexes which contain specific binding sites of rat liver chromatin particles as affected by cortisol].
Using a differential non-detergent phenol-salt deproteinization technique, the DNA fractions corresponding to functionally different parts of the genome, i.e., DNA I (transcriptionally active DNA), DNA II (stably repressed genome DNA), and DNA III (potentially active genome DNA) were obtained. Under conditions of cortisol-induced activation of transcription in rat liver cells, the number or repetitive sequences in DNA I (represented by DNA III in intact animals) increased. In this way, the hormone-induced transcription appeared to be associated with a transition to a transcriptionally active state of chromatin containing repetitive sequences that exist in intact liver in potentially active parts of the genome. It was shown that these inducible repetitive sequences contain binding sites for glucocorticoid-receptor complexes. In DNA III, these sites are present in large amounts in the sequences within the composition of stable DNA-protein complexes, which suggests the specificity of DNA sequences firmly bound to chromatin proteins.
D000818:Animals; D001667:Binding, Competitive; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D006854:Hydrocortisone / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects
D002843:Chromatin; D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D004247:DNA; D006854:Hydrocortisone
Izmenenie sostoianiia soderzhashchikh saĭty spetsificheskogo sviazyvaniia gormon-retseptornykh kompleksov uchastkov khromatina pecheni krys pod deĭstviem kortizola.