title: An encapsidated, subgenomic messenger RNA encodes the coat protein of carnation mottle virus.
abstract: The translation strategy of carnation mottle virus (CarMV) in vitro has been generally assumed to involve internal initiation events on full-length, genomic RNA (4.3 kb). We suggest that this is, at least in part, incorrect. Encapsidated RNA, fractionated on denaturing sucrose gradients, or total RNA from CarMV-infected leaves, fractionated under non-denaturing conditions, was translated in an mRNA-dependent rabbit reticulocyte cell-free system. Evidence for subgenomic RNAs which encode a polypeptide of Mr 38 000 was found. This product was shown to be related to authentic CarMV coat protein by partial proteolysis with alpha-chymotrypsin and SDS/polyacrylamide-gel electrophoresis.
mesh_terms: D002213:Capsid / Q000235:genetics* / Q000378:metabolism; D005796:Genes; D005814:Genes, Viral; D010446:Peptide Fragments / Q000032:analysis; D010942:Plant Viruses / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000302:isolation & purification; D014771:Virion / Q000235:genetics
chemical_list: D010446:Peptide Fragments; D012333:RNA, Messenger; D012367:RNA, Viral