title: Effects of local anesthetics on phorbol ester-induced ornithine decarboxylase activity in mouse and human skin.
abstract: In preparation for experiments to determine the effects of various orally administered compounds on human skin ornithine decarboxylase (ODC) activity, it was observed that intradermal lidocaine hydrochloride inhibited 12-0-tetradecanoylphorbol-13-acetate (TPA)-induced ODC. Topically applied ethyl chloride had variable and unpredictable effects on ODC induction. Adequate local anesthesia can be obtained by using intradermal 1.0% procaine hydrochloride or normal saline; neither of these agents inhibited TPA induction of epidermal ODC activity in incubated human skin punch biopsies.
mesh_terms: D000779:Anesthetics, Local / Q000494:pharmacology*; D000818:Animals; D001706:Biopsy / Q000379:methods; D004311:Double-Blind Method; D004790:Enzyme Induction / Q000187:drug effects; D005018:Ethyl Chloride / Q000494:pharmacology; D005260:Female; D006801:Humans; D008012:Lidocaine / Q000494:pharmacology; D051379:Mice; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D010703:Phorbol Esters / Q000037:antagonists & inhibitors*; D010704:Phorbols / Q000037:antagonists & inhibitors*; D011343:Procaine / Q000494:pharmacology; D011897:Random Allocation; D012867:Skin / Q000187:drug effects* / Q000201:enzymology; D012965:Sodium Chloride / Q000494:pharmacology; D013048:Specimen Handling
chemical_list: D000779:Anesthetics, Local; D010703:Phorbol Esters; D010704:Phorbols; D012965:Sodium Chloride; D005018:Ethyl Chloride; D011343:Procaine; D008012:Lidocaine; D009955:Ornithine Decarboxylase