title: Improved gas chromatographic method for quantitation of deoxynivalenol in wheat, corn, and feed.
abstract: A gas chromatographic (GC) method is described to determine deoxynivalenol in wheat and corn at levels as low as 20 ppb. Ground samples are extracted with water, adsorbed onto a Clin Elut column, extracted with ethyl acetate, and passed through a silica gel Sep-Pak cartridge. The final extract is then derivatized with N-heptafluorobutyrylimidazole and quantitated by GC using an electron capture detector. Recoveries are greater than 85% for spiked samples at levels of 50-1000 ppb. Results for wheat, corn, and mixed feed samples are given as well as the results of an interlaboratory study on a naturally contaminated wheat sample.
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas / Q000379:methods; D005516:Food Microbiology; D009183:Mycotoxins / Q000032:analysis*; D012717:Sesquiterpenes / Q000032:analysis*; D014255:Trichothecenes / Q000032:analysis*; D014908:Triticum / Q000032:analysis*; D003313:Zea mays / Q000032:analysis*
chemical_list: D009183:Mycotoxins; D012717:Sesquiterpenes; D014255:Trichothecenes; C007262:deoxynivalenol