Simultaneous high-performance liquid chromatography assay of acetylsalicylic acid and salicylic acid in film-coated aspirin tablets.
A reversed-phase high-performance liquid chromatography (HPLC) method has been developed for the simultaneous assay of acetylsalicylic acid (I) and salicylic acid (II) in film-coated aspirin tablets. As little as 0.1% II (relative to I) can be quantitatively determined. Using a 5-microns octadecylsilane column with water-acetonitrile-phosphoric acid (76:24:0.5) as the mobile phase enabled the chromatographic separation to be completed in 4 min. Due to the slow rate of decomposition of I to II in the extraction solvent, acetonitrile-methanol-phosphoric acid (92:8:0.5), the analysis of many samples was routinely performed by means of automated HPLC equipment. Other compounds (non-aspirin salicylates, caffeine and acetaminophen) were also separated by the chromatographic system.
D001241:Aspirin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D012459:Salicylates / Q000032:analysis*; D020156:Salicylic Acid; D013056:Spectrophotometry, Ultraviolet; D013608:Tablets, Enteric-Coated / Q000032:analysis
D012459:Salicylates; D013608:Tablets, Enteric-Coated; D020156:Salicylic Acid; D001241:Aspirin