Developmental regulation of production of an aggregation-stimulating factor from the cellular slime mold Polysphondylium violaceum.
An excreted, dialyzable component(s) produced during development of wild-type Polysphondylium violaceum has been previously shown to stimulate aggregation of aggregateless mutants in the complementation group aggA. Production of this aggregation-stimulating factor, called D factor, is greater during development in liquid culture than during development on a surface. after partial purification of crude D factor using high performance liquid chromatography, multiple species are found that retain the ability to stimulate aggregation of the aggA mutants. The three major components (DfA, DfB, and DfC) show decreasing polarity based on purification using reverse-phase chromatography. The proportion of each component secreted varies, depending on the developmental conditions (surface versus liquid) and the time after starvation when the factors are isolated. Preliminary physical and chemical characterization of the three D factor components suggests that they are related.
D015815:Cell Adhesion Molecules; D002851:Chromatography, High Pressure Liquid; D009235:Myxomycetes / Q000254:growth & development / Q000502:physiology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification; D013997:Time Factors
D015815:Cell Adhesion Molecules; D011506:Proteins; C013065:cell aggregation factors