Autometallography. A new technique for light and electron microscopic visualization of metals in biological tissues (gold, silver, metal sulphides and metal selenides).
The autometallographic procedure represents a new technique that can substitute for the normal methods of physical development (PD). The physical developer (a solution of reducing substance, silver salt and protection colloid) is replaced by a photographic emulsion and chemical developer. Accumulations of gold, silver, metal sulphides and metal selenides can be amplified by the present technique. Tissue sections placed on glass slides are covered by a silver bromide containing emulsion, dried and exposed to a chemical developer. After development the emulsion is either removed or cleared and the sections are counterstained and embedded. The autometallographic procedure can also be applied to ultrathin sections.
D000818:Animals; D006046:Gold / Q000378:metabolism; D006651:Histocytochemistry / Q000379:methods*; D008670:Metals / Q000378:metabolism*; D008854:Microscopy, Electron; D051381:Rats; D012643:Selenium / Q000378:metabolism; D018036:Selenium Compounds; D012834:Silver / Q000378:metabolism; D013440:Sulfides / Q000378:metabolism
D008670:Metals; D018036:Selenium Compounds; D013440:Sulfides; C037341:sodium selenide; D012834:Silver; D006046:Gold; D012643:Selenium; C033479:sodium sulfide