1H-NMR spectroscopy of the glycoprotein-bound large carbohydrates from embryonal carcinoma cells.
400 MHz NMR spectrum was recorded for the glycoprotein -bound large carbohydrates (embryoglycan) isolated from F9 embryonal carcinoma cells. Two intense signals at 4.13 ppm and 4.69 ppm were assigned to be H-4 of galactosyl residues substituted at C-3 and H-1 of G1cNAc beta 1----3, respectively. The result is consistent with the proposal that the fundamental building unit of the large glycan is G1cNAc beta 1----3Ga1 beta. Furthermore, the spectral data confirmed a conclusion obtained by glycosidase digestion that fucosyl residues are linked mostly to N-acetylglucosamine rather than galactose.
D002240:Carbohydrate Sequence; D054278:Embryonal Carcinoma Stem Cells; D006023:Glycoproteins / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D009363:Neoplasm Proteins / Q000032:analysis*; D014411:Neoplastic Stem Cells / Q000032:analysis*; D013234:Stem Cells / Q000032:analysis*; D013724:Teratoma / Q000032:analysis*
D006023:Glycoproteins; D009363:Neoplasm Proteins