Design of flow chamber with electronic cell volume capability and light detection optics for multilaser flow cytometry.
A multibeam optical detection system has been developed with a high optical efficiency, achieved through a reduction in the number of optical interfaces employed in the system. This reduction is made possible by a combination of employing simple lenses, gluing the objective lens directly upon the face of the flow cuvette and the extraction of only one fluorescence signal from each laser beam. A modified flow chamber is also described that includes fluidic resistance elements for the elimination of most of the electric shielding normally associated with electronic cell volume measurements.
D000818:Animals; D002455:Cell Division; D002477:Cells / Q000166:cytology*; D002645:Chickens; D004911:Erythrocyte Volume; D004912:Erythrocytes / Q000166:cytology; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D007834:Lasers; D008223:Lymphoma / Q000473:pathology; D051379:Mice