Preferential nuclease digestion of the non-messenger sequences of HnRNA upon micrococcal nuclease digestion of HnRNP particles.
HnRNP particles from rat liver were digested with micrococcal nuclease to render about 50% of HnRNA acid soluble. Hybridization experiments, in which cDNA complementary to rat liver poly(A)+ mRNA was annealed to an excess of HnRNA, showed that the micrococcal nuclease resistant HnRNA fraction was enriched in mRNA-homologous sequences.
D000818:Animals; D001483:Base Sequence; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D008297:Male; D008836:Micrococcal Nuclease / Q000494:pharmacology*; D012332:RNA, Heterogeneous Nuclear / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012261:Ribonucleoproteins / Q000032:analysis*
D034441:Heterogeneous-Nuclear Ribonucleoproteins; D012332:RNA, Heterogeneous Nuclear; D012261:Ribonucleoproteins; D008836:Micrococcal Nuclease