Liquid chromatographic determination of nicarbazin in feeds and premixes.
A liquid chromatographic (LC) method has been developed for the determination of nicarbazin in premixes and poultry feed. Liquid chromatography of the 4,4'-dinitrocarbanilide (DNC) portion of nicarbazin is performed isocratically with a reverse phase octadecylsilica column and a UV detector set at 340 nm. The 2-hydroxy-4,6-dimethylpyrimidine (HDP) portion of nicarbazin is chromatographed isocratically with a reverse phase octylsilica column and a UV detector set at 305 nm. Nicarbazin concentration can be calculated by assaying both DNC and HDP, or by assaying DNC or HDP and assuming that nicarbazin is a 1:1 molar ratio of the two. Average recoveries of DNC and HDP added to poultry feed were 101% and 87%, respectively. This procedure provides an alternative to existing colorimetric procedures for determining nicarbazin in premixes and poultry feeds.
D000821:Animal Feed / Q000032:analysis*; D002224:Carbanilides / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D005503:Food Additives / Q000032:analysis*; D009528:Nicarbazin / Q000032:analysis*; D011744:Pyrimidinones / Q000032:analysis
D002224:Carbanilides; D005503:Food Additives; D011744:Pyrimidinones; C044189:2-hydroxy-4,6-dimethylpyrimidine; D009528:Nicarbazin; C039493:4,4'-dinitrocarbanilide