[Proliferative activity of cultures of embryonic mouse fibroblasts, infected by CA7(C8) adenovirus in a non-serum medium].
Primary monolayer cultures of mouse fetal cells were grown in serum-depleted medium. Cell proliferation parameters were measured by scanning cytophotometry and 3H-thymidine autoradiography. Both 1 h and 24 h thymidine labelling indices (LI) were extremely low in cultures growing without serum for 1 day indicating only residual proliferative activity. The number of cytophotometrically detected S-phase cells was slightly higher than the corresponding LI values. This suggests either the arrest of the cells in S-phase or failure to utilize exogenous thymidine without serum. 24 h after infection by oncogenic adenovirus SA7 (C8) a burst of proliferative activity was revealed in serum-depleted cultures with a dramatic increase of both LI values and the amount of S-cells detected by cytophotometry. These data are discussed in terms of possible virus-induced neoplastic cell transformation.
D000256:Adenoviridae; D000261:Adenoviruses, Simian; D000818:Animals; D001345:Autoradiography; D002455:Cell Division; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003470:Culture Media; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008808:Mice, Inbred CBA; D008940:Mitotic Index; D051381:Rats
D003470:Culture Media
Proliferativnaia aktivnost' kul'tur fibroblastov émbrionov mysheĭ, zarazhennykh adenovirusom CA7 (C8), v bessyvorotochnoĭ srede.