title: Phosphate repression of phage protein synthesis during infection by choleraphage phi 149.
abstract: A synthetic medium for choleraphage phi 149 growth, in which the concentration of phosphate ions plays a significant role, has been defined. Upon infection, choleraphage phi 149 DNA binds to the cell membrane at three to four sites. The host macromolecular syntheses are shut off by 10 min after infection and the synthesis of phage-specific DNA is detectable after 20 min of infection. The phage utilizes primarily the host DNA degradation products for its own DNA synthesis. When added during the first 20 min of infection both nalidixic acid and novobiocin inhibit phage growth. The effects of these antibiotics are not pronounced when added late during infection. Pulse labeling of ultraviolet-irradiated infected cells at different times during infection has allowed identification of about 50 phage-specific proteins of which 19 are structural proteins. These proteins appear during the infection cycle in two distinct phases, early and late. When infection is carried out in high-phosphate medium, none of the late proteins is synthesized. Eleven of the 26 early proteins detected are DNA-binding proteins.
mesh_terms: D001435:Bacteriophages / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003470:Culture Media; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D009268:Nalidixic Acid / Q000494:pharmacology; D009675:Novobiocin / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology*; D013997:Time Factors; D014734:Vibrio cholerae / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis*; D015678:Viral Structural Proteins
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D010710:Phosphates; D014764:Viral Proteins; D015678:Viral Structural Proteins; D009675:Novobiocin; D009268:Nalidixic Acid