Denitrosation of diphenylnitrosamine in vivo.
A single dose of diphenylnitrosamine (NDphA) was applied orally or intraperitoneally (i.p.) to rats. The urine was sampled and analysed for nitrite/nitrate by ion chromatography and for diphenylamine (DphA) plus hydroxydiphenylamine by gas chromatography. The major metabolite was nitrate. Nitrite and DphA were found in minor amounts. In a somewhat higher concentration, a monohydroxylated DphA was detected. It is concluded that NDphA is denitrosated to nitric oxide (NO) and DphA in the organism and that NO is then converted into nitrite and nitrate.
D000284:Administration, Oral; D000818:Animals; D001711:Biotransformation; D002852:Chromatography, Ion Exchange; D004159:Diphenylamine / Q000652:urine; D005260:Female; D009566:Nitrates / Q000652:urine; D009573:Nitrites / Q000652:urine; D009602:Nitrosamines / Q000378:metabolism* / Q000652:urine; D009603:Nitroso Compounds / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains
D009566:Nitrates; D009573:Nitrites; D009602:Nitrosamines; D009603:Nitroso Compounds; D004159:Diphenylamine; C023649:N-nitrosodiphenylamine