Interference by cold agglutinins with the measurement of particle concentrations in the Coulter Counter Model S-Plus IV.
Cold agglutinins may interfere with measurement of red cell indices in the Coulter Counter Model S-Plus IV due to aggregation of erythrocytes. This is most easily detected by a mean erythrocyte haemoglobin concentration (MCHC) raised above 22.7 mmol/l (established by experience). Heating of the samples will normalize the values in most cases. Temperature-dependent red cell indices were not found in approximately 100 samples with MCHC between 19.5 mmol/l and 22.7 mmol/l. Aggregation of and interference with measurement of white cells and platelets has not been observed.
D000373:Agglutinins; D001772:Blood Cell Count; D001774:Blood Chemical Analysis / Q000295:instrumentation; D003450:Cryoglobulins; D004903:Erythrocyte Aggregation; D004909:Erythrocyte Indices; D006801:Humans
D000373:Agglutinins; D003450:Cryoglobulins; C029443:cold agglutinins