[Intranuclear localization of hepatoma chromatin antigens not detected in liver chromatin].
Localization of malignant cell antigens which are not detected in the liver chromatin was investigated by antibodies to chromatin of Zajdela ascite hepatoma and solid hepatoma 27. Antibodies to chromatin of Zajdela ascite hepatoma do not interact with nuclear matrix of both hepatoma and liver cells. Zajdela ascite hepatoma and solid hepatoma 27 chromatin regions hypersensitive to DNase I and endogenous Mg2+-dependent nuclease are enriched with immunogenic proteins. Antibodies to hepatoma chromatins pretreated with liver chromatin show that hepatoma chromatin antigens which are not detected in liver chromatin are localized in chromatin regions hypersensitive to nucleases but are absent (or scanty) in actively transcribed regions.
D000818:Animals; D000912:Antibodies, Neoplasm / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis*; D002843:Chromatin / Q000032:analysis / Q000276:immunology*; D004273:DNA, Neoplasm / Q000276:immunology; D003850:Deoxyribonuclease I / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology; D008099:Liver / Q000201:enzymology / Q000276:immunology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000276:immunology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D002843:Chromatin; D004273:DNA, Neoplasm; D007074:Immunoglobulin G; D003850:Deoxyribonuclease I
Vnutriiadernaia lokalizatsiia antigenov khromatina gepatom, ne vyiavliaemykh v khromatine kletok pecheni.