The behaviour of urokinase and porcine kidney cell plasminogen activator towards some synthetic peptides.
The behaviour of human urokinase and porcine kidney cell plasminogen activator towards some synthetic substrates has been investigated. Although N- benzyloxycarbonylglycylglycyl -L-arginine 4-methyl-7- coumarylamide (Z-Gly-Gly-Arg-Amc) (I), glutaryl-Gly-Arg-Amc (II) and Z-Gly-Gly-Arg-Val-OMe (III) were substrates, Boc-Gly-Gly-Arg-Val-Val-Gly-Gly-OEt (IV) and Z-Ala-Pro-Gly-Arg-Val-Val-Gly-Gly-OEt (V) were neither substrates nor inhibitors. Steady-state kinetic parameters for the hydrolysis of (II) and (III) by urokinase and porcine kidney cell plasminogen activator were similar.
D000818:Animals; D003374:Coumarins / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D009842:Oligopeptides / Q000378:metabolism*; D010960:Plasminogen Activators / Q000378:metabolism*; D013552:Swine; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism*
D003374:Coumarins; D009842:Oligopeptides; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator