title: Fractionation of human serum proteins by immobilized metal affinity chromatography.
abstract: Human serum was fractionated on zinc and cadmium iminodiacetate and tris(carboxymethyl)ethylenediamine chelates. Zinc iminodiacetate was found to be useful in the isolation of hemopexin and alpha 2-macroglobulin. The chromatographic behaviour of serum proteins on zinc iminodiacetate could be modulated by the nature of the eluent; a sulphate-containing solvent increased the binding of serum proteins to immobilized metal ions.
mesh_terms: D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000302:isolation & purification*; D002614:Chelating Agents; D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008670:Metals; D013696:Temperature
chemical_list: D001798:Blood Proteins; D002614:Chelating Agents; D008670:Metals