Qualitative detection of corticosteroids in equine biological fluids and the comparison of relative dexamethasone metabolite/dexamethasone concentration in equine urine by micro-liquid chromatography-mass spectrometry.
Several important corticosteroids were qualitatively determined in the plasma and urine of horses by micro-liquid chromatography-mass spectrometry (micro-LC-MS). The sensitivity and specificity of micro-LC-MS are demonstrated as is the ability of micro-LC-MS to deal with endogenous interferences. In turn, the relative amount of dexamethasone and its major unconjugated metabolite were determined in equine urine by micro-LC-MS; the conclusions drawn are reported.
D000305:Adrenal Cortex Hormones / Q000032:analysis* / Q000097:blood / Q000652:urine; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D003907:Dexamethasone / Q000652:urine*; D006736:Horses; D013058:Mass Spectrometry / Q000379:methods
D000305:Adrenal Cortex Hormones; D003907:Dexamethasone