Naturally-occurring cellular cytotoxicity mediated by neutrophil polymorphonuclears: requirements for the target cell lysis.
The purpose of the present study was to investigate the general conditions under which neutrophil polymorphonuclears (PMN) mediate antibody-independent cytolysis in the presence of normal human serum (NHS). Normal PMN were found to kill rabbit red blood cells (RRBC) only when cultured with 1% NHS. NHS was per se incapable of lysing RRBC. PMN from a patient with Chronic Granulomatous Disease did not destroy RRBC targets even in the presence of 1% NHS. In addition, cytotoxicity by normal PMN was significantly reduced by scavengers of oxygen metabolites. The results suggest that the target cell lysis by PMN in the presence of NHS requires a synergistic interaction between at least two mediators: serum factor(s) and oxygen metabolite(s).
D000818:Animals; D003470:Culture Media; D003602:Cytotoxicity, Immunologic; D004912:Erythrocytes / Q000276:immunology; D006105:Granulomatous Disease, Chronic / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate; D066298:In Vitro Techniques; D009504:Neutrophils / Q000276:immunology*; D010100:Oxygen / Q000378:metabolism; D011817:Rabbits
D003470:Culture Media; D010100:Oxygen