[Effect of chloramphenicol and actinomycin D on extracellular alkaline RNAse biosynthesis by Bacillus intermedius].
By means of chloramphenicol it was found that biosynthesis of alkaline exocellular RNAase was repressed in Bacillus intermedius by inorganic phosphate. Actinomycin D at a low concentration stimulates RNAase biosynthesis in a medium with a minimal phosphorus concentration in model experiments with washed cells and in the batch culture. As a result, the activity of RNAase rises 2-4 times. The stimulating effect of actinomycin D decreases when phosphorus concentration in the medium is increased The effect of actinomycin D is maximal if the antibiotic is added to the medium when the specific growth rate of the bacterium falls down and the rate of RNAase biosynthesis rises.
D001407:Bacillus / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D002701:Chloramphenicol / Q000494:pharmacology*; D003470:Culture Media / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005095:Exoribonucleases / Q000096:biosynthesis*; D010710:Phosphates / Q000378:metabolism; D012260:Ribonucleases / Q000096:biosynthesis*; D013997:Time Factors
D003470:Culture Media; D010710:Phosphates; D003609:Dactinomycin; D002701:Chloramphenicol; D005095:Exoribonucleases; D012260:Ribonucleases
Vliianie khloramfenikola i aktinomitsina D na biosintez shchelochnoĭ vnekletochnoĭ RNKazy Bacillus intermedius.