title: Gas chromatography-mass spectrometry characteristics of methylated perfluoroacyl derivatives of cytosine and 5-methylcytosine.
abstract: Pentafluorobenzoyl and heptafluorobutyryl derivatives of cytosine and pentafluorobenzoyl-5-methylcytosine have been examined by mass spectrometry following permethylation. Electron impact mass spectra were used to confirm the structures of the derivatives. The permethylated pentafluorobenzoyl derivatives of cytosine and 5-methylcytosine are sensitive for quantitative analysis by capillary gas chromatography-mass spectrometry. Under negative ion chemical ionization conditions 1 femtogram of each is detected.
mesh_terms: D044503:5-Methylcytosine; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis*; D005464:Fluorobenzenes / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods
chemical_list: D005464:Fluorobenzenes; D044503:5-Methylcytosine; D003596:Cytosine