Cloning gene ura5 for the orotidylic acid pyrophosphorylase of the filamentous fungus Podospora anserina: transformation of protoplasts.
From a genomic library of the filamentous fungus Podospora anserina, we have cloned a 4.9-kb fragment which complements an Escherichia coli mutant strain deficient for orotidylic acid pyrophosphorylase (pyrE gene). The recombinant plasmid pPAura5 also transforms to prototrophy a mutant strain of P. anserina carrying a mutation in the ura5 gene and lacking OMPppase activity.
D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics*; D005658:Fungi / Q000235:genetics; D005800:Genes, Fungal; D009154:Mutation; D009962:Orotate Phosphoribosyltransferase / Q000235:genetics*; D010430:Pentosyltransferases / Q000235:genetics*; D010957:Plasmids; D014170:Transformation, Genetic
D004271:DNA, Fungal; D010430:Pentosyltransferases; D009962:Orotate Phosphoribosyltransferase