Rapid screening and confirmation for drugs and metabolites in racing animals by tandem mass spectrometry.
A screening and confirmation procedure for drugs and metabolites in the blood serum and urine of racing animals was developed. Equine blood serum was spiked with low concentrations of several drugs of interest. Canine blood serum and urine were collected following oral doses of diethylcarbamazine, procaine, and phenylbutazone. Serum, urine, and extracts of each were analyzed, using a triple quadrupole mass spectrometer. Simultaneous screening of up to 50 drugs was possible in a single sample, in less than 2 minutes. Detection limits for most compounds were in the ng/ml to microgram/ml range, using 1-microliter samples. This procedure provided fast, sensitive screening for selected drugs and metabolites in blood serum and urine.
D000818:Animals; D004049:Diethylcarbamazine / Q000378:metabolism; D004285:Dogs / Q000378:metabolism*; D006736:Horses / Q000378:metabolism*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D010653:Phenylbutazone / Q000378:metabolism; D011343:Procaine / Q000378:metabolism; D012420:Running
D004364:Pharmaceutical Preparations; D011343:Procaine; D010653:Phenylbutazone; D004049:Diethylcarbamazine