title: The effect of anaesthesia and surgery for benign disease of the upper urinary tract on circulating leucocyte subpopulations identified with monoclonal antibodies.
abstract: Circulating lymphocyte subpopulations were monitored, using monoclonal antibodies and flow cytometry, in six patients undergoing surgery for benign disease of the upper urinary tract. A significant decrease in the total number of circulating lymphocytes was observed. This could be attributed to a significant decrease of T cells of both major subsets--the so-called T "helper" (Th) and T "suppressor/cytotoxic" (Ts) subpopulations. When the results of the T cell subsets were expressed as a ration (leu-3a+/leu-2a+, T "helper/suppressor") no significant change was noted. In contrast neither B cells nor natural killer (NK) and antibody dependent killer (K) cells were significantly affected. This selective loss of T cells from the circulation may be relevant to post operative infection and should be considered in the course of immunological monitoring.
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000758:Anesthesia / Q000009:adverse effects; D000911:Antibodies, Monoclonal / Q000276:immunology; D002469:Cell Separation; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000145:classification* / Q000276:immunology; D007970:Leukopenia / Q000209:etiology*; D008297:Male; D008875:Middle Aged; D011183:Postoperative Complications / Q000276:immunology*; D014551:Urinary Tract / Q000601:surgery*
chemical_list: D000911:Antibodies, Monoclonal