A mechanism for the selective preservation of homogeneous. F(ATP) actin.
The cold-induced depolymerization of F(ADP) actin, coupled with the repolymerization by ATP, was employed to study the formation of F(ATP) actin. It is proposed that the cold-induced lability of F(ADP) actin, together with the spontaneous fragmentation of the filament, provides a mechanism for the selective preservation of homogeneous F(ATP) actin.
D000199:Actins / Q000302:isolation & purification* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003080:Cold Temperature; D006868:Hydrolysis; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D011108:Polymers; D011485:Protein Binding; D011817:Rabbits
D000199:Actins; D011108:Polymers; D000255:Adenosine Triphosphate; D008274:Magnesium