Determination of phosphoethanolamine in urine or in the presence of high taurine concentrations.
Phosphoethanolamine forms a low-solubility lead salt, which may be precipitated from urine together with inorganic phosphate, thus separating taurine, urea, and other interfering substances of chromatographic determination. Fluorometric detection of phosphoethanolamine with o-phthaldialdehyde after elution from a Dowex 50-X10 column enhances the specificity of the method once more. The method described is fast and easy to perform.
D011232:Chemical Precipitation; D002852:Chromatography, Ion Exchange; D004983:Ethanolamines / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007854:Lead; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013654:Taurine / Q000652:urine*
D004983:Ethanolamines; D013654:Taurine; D007854:Lead; C005448:phosphorylethanolamine