title: Effect of dog food containing 460 ppm fluoride on rat reproduction.
abstract: A one-generation, two-litter rat reproduction study was done in a kennel in Allegan County, Michigan, as part of an investigation of reproductive problems in Shetland sheepdogs (shelties). Since 1970, at least 115 sheltie litters at this kennel have been grossly deformed and/or have died, generally within 3 d of birth. The causative factor(s) appears to be associated with the environment, as the shelties have had successful pregnancies elsewhere. There was some concern that a potential threat to human reproduction also might exist in this area. After it was discovered that fluoride in the dog food had apparently caused mottled teeth and bony exostoses in dogs at this and at least two other kennels, dog-food fluoride content and local well water were investigated as possible causes of the reproductive problems. A two-way factorial statistical design allowed assessment of dietary fluoride content and water source and interaction between the two. UPj:TUC(SD)spf rats, 9 males and 18 females in each group, were assigned to treatment with high-fluoride dog food (460 ppm) and well water; high-fluoride dog food and distilled water; low-fluoride dog food (56 ppm) and well water; and low-fluoride dog food and distilled water. After 60 d in the kennel, the rats were mated. Even after two litters, the only adverse effect was dental fluorosis in the high-fluoride groups. The results indicated that rats cannot be used in the search for the cause(s) of reproductive problems in dogs in this kennel.
mesh_terms: D000014:Abnormalities, Drug-Induced / Q000662:veterinary; D000821:Animal Feed / Q000633:toxicity*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004283:Dog Diseases / Q000139:chemically induced; D004285:Dogs; D005260:Female; D005459:Fluorides / Q000378:metabolism / Q000633:toxicity*; D008297:Male; D011247:Pregnancy; D051381:Rats; D012098:Reproduction / Q000187:drug effects*; D014881:Water Supply
chemical_list: D005459:Fluorides