Radioimmunoassay of 5-methyl-2'-deoxycytidine. A method for the quantitation of DNA methylation.
5-Methyl-2'-deoxycytidine (5MedCyd) is a minor constituent of mammalian cell DNA. We report the production and characterization of highly specific rabbit anti-5MedCyd antiserum. The antiserum was suitable for the radioimmunological measurement of 5MedCyd. This simple radioimmunoassay was capable of quantitating calf thymus DNA methylation at nanomolar levels of total DNA.
D000818:Animals; D002250:Carbon Radioisotopes; D002417:Cattle; D004247:DNA / Q000032:analysis*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000032:analysis; D003850:Deoxyribonuclease I; D006868:Hydrolysis; D007106:Immune Sera; D007700:Kinetics; D008745:Methylation; D011863:Radioimmunoassay / Q000379:methods; D013950:Thymus Gland
D002250:Carbon Radioisotopes; D007106:Immune Sera; D003841:Deoxycytidine; D004247:DNA; C016569:5-methyldeoxycytidine; D003850:Deoxyribonuclease I