The intracellular calcium antagonist TMB-8 [8-(NN-diethylamino)octyl-3,4,5-trimethoxybenzoate] inhibits mitochondrial ATP production in rat thymocytes.
TMB-8 inhibited respiration of rat thymocytes and rat liver mitochondria, probably by inhibition of NADH dehydrogenase. TMB-8 markedly decreased both the cellular ATP concentration and the mitochondrial membrane potential in situ in thymocytes. These effects occurred at, or well below, the concentrations used in other systems to investigate the role of intracellular calcium pools in signalling events. We conclude that caution should be exercised in the interpretation of the effects of TMB-8.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D009861:Onium Compounds / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014320:Triphenylmethyl Compounds / Q000378:metabolism
D009861:Onium Compounds; D014320:Triphenylmethyl Compounds; C011561:triphenylmethylphosphonium; C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D005707:Gallic Acid; D000255:Adenosine Triphosphate