title: Esterase-23 (ES-23): characterization of a new carboxylesterase isozyme (EC 3.1.1.1) of the house mouse, genetically linked to ES-2 on chromosome 8.
abstract: Genetic variation of a carboxylesterase isozyme (EC 3.1.1.1) of the house mouse, designated ES-23, is described. ES-23 was found in kidney, liver, and intestine. The isozyme was resistant to inhibition by 10(-3) mol/liter eserine and was stained using alpha-naphthyl butyrate or 5-bromoindoxyl acetate as substrate. Five different phenotypes, ES-23A to ES-23E, could be distinguished by disc electrophoresis and by isoelectric focusing. ES-23 is controlled by a structural locus situated within the esterase gene cluster 2 on chromosome 8. An analysis of allele distribution among different strains suggested a separate structural locus for the isozyme, Es-23e, which is closely linked to the loci Es-2, Es-5, Es-7, and Es-11. Of the five phenotypes, only ES-23B was expressed in lung. This variation is apparently controlled by a cis-acting regulatory element, presumably a temporal locus, Es-23t, closely linked to the presumed structural locus Es-23e.
mesh_terms: D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors / Q000235:genetics*; D002874:Chromosome Mapping; D008040:Genetic Linkage; D007527:Isoenzymes / Q000235:genetics*; D046911:Macromolecular Substances; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics* / Q000254:growth & development; D008970:Molecular Weight; D010641:Phenotype; D014018:Tissue Distribution
chemical_list: D007527:Isoenzymes; D046911:Macromolecular Substances; D002265:Carboxylic Ester Hydrolases