title: Three-dimensional reconstruction from tilted sections of fish muscle M-band.
abstract: Fish muscle provides a particularly suitable specimen for studying the organization of thick filaments in vertebrate striated muscle because the filaments are arranged with a single orientation on a well ordered hexagonal lattice. The M-band consists of sets of cross-links, which join the myosin filaments in the middle of the A-band. Previous work concluded that the fish muscle M-band had the local symmetry of the dihedral point group 32. We present here a quantitative analysis of transverse sections of the M-band, using general methods developed for crystalline layers, to combine various tilted views. The three-dimensional map computed to a resolution of about 70 A confirms previous findings; it shows new features of the thick filament structure in the M-band region and provides new information on the use of three-dimensional reconstruction from sectioned biological material. The accompanying paper describes a novel technique of three-dimensional reconstruction of the fish M-band from a single view of a slightly oblique plastic section.
mesh_terms: D000818:Animals; D003599:Cytoskeleton / Q000648:ultrastructure*; D005399:Fishes; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D009132:Muscles / Q000648:ultrastructure*; D009218:Myosins; D011485:Protein Binding
chemical_list: D046911:Macromolecular Substances; D009218:Myosins