Separation and characterization of lymphocytes from rat lung parenchyma.
Rat pulmonary parenchymal tissue was disaggregated into a single cell suspension by treatment with collagenase. A cell population enriched for lung lymphocytes was separated by depletion of adherent cells on a Sephadex G-10 column: 16.7 +/- 2.7 X 10(6) cells per animal were recovered. On the basis of cytochemical and morphologic criteria, the separated cells contained greater than 80% lymphocytes, with a viability of 80-90%. The distribution of lymphocyte subpopulations was determined by indirect immunoperoxidase staining with appropriate monoclonal antibodies. Separated lung lymphocytes exhibited a proliferative response in vitro to phytohemagglutinin and concanavalin A. Supernatants from lung lymphocytes stimulated with concanavalin A contained lymphokine activity that could be demonstrated in a leukocyte procoagulant activity assay.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation / Q000379:methods*; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D008168:Lung / Q000166:cytology*; D008214:Lymphocytes / Q000166:cytology* / Q000378:metabolism; D008222:Lymphokines / Q000096:biosynthesis; D010835:Phytohemagglutinins / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D008222:Lymphokines; D010835:Phytohemagglutinins; D003208:Concanavalin A