Locomotion and cell surface movements of fibroblasts in fibrillar collagen gels.
We have used light microscopy and scanning, scanning transmission, transmission, and high voltage electron microscopy to study the shape and cell surface movements of chick embryo fibroblasts cultured in Vitrogen collagen gels. Cells in gels are less well spread than their counterparts on glass but they retain a dorso-ventral polarity, and move by extending long, narrow lamellar processes. We show here for the first time that cells moving in 3D substrata form perinuclear caps of cross-linked cell surface receptors.
D000818:Animals; D002465:Cell Movement; D002642:Chick Embryo; D003094:Collagen; D005347:Fibroblasts / Q000166:cytology*; D005782:Gels; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning
D005782:Gels; D003094:Collagen