Direct, simultaneous determination of propranolol and its 4-hydroxy metabolite by liquid chromatography.
Propranolol and its active 4-hydroxy metabolite are determined by direct injection of spiked plasma samples onto a protein-coated ODS liquid chromatographic column. The recovery of propranolol and the 4-hydroxy metabolite is essentially quantitative and the reproducibility is good. The reproducibility and simplicity may be superior to the conventional HPLC analyses which include pretreatment, i.e., solvent extraction or deproteinization.
D002853:Chromatography, Liquid; D006801:Humans; D011433:Propranolol / Q000031:analogs & derivatives* / Q000097:blood*
C037602:N-hydroxypropranolol; D011433:Propranolol