Effects of ammonium chloride on resorption of fetal rat bones in organ culture.
Ammonium chloride, a known inhibitor of lysosomal function, was found to be a rapid and potent inhibitor of 45Ca release from fetal rat bones in organ culture. The response to parathyroid hormone and prostaglandin E2 was inhibited in a dose-related, reversible fashion. The activity of the lysosomal enzyme beta-glucuronidase in the medium closely paralleled 45Ca release. Ammonium chloride may now be added to the list of antilysosomal agents that inhibit bone resorption in vitro.
D000643:Ammonium Chloride / Q000494:pharmacology*; D000818:Animals; D001842:Bone and Bones / Q000196:embryology / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D005260:Female; D005313:Fetal Death / Q000139:chemically induced*; D005327:Fetal Resorption / Q000139:chemically induced*; D005966:Glucuronidase / Q000378:metabolism; D009924:Organ Culture Techniques; D010281:Parathyroid Hormone / Q000494:pharmacology; D011247:Pregnancy; D051381:Rats / Q000196:embryology; D013997:Time Factors
D010281:Parathyroid Hormone; D000643:Ammonium Chloride; D005966:Glucuronidase; D002118:Calcium