A new method for sister-chromatid exchange studies in vivo, a single injection of BrdU in melted agar.
Details are given for a new, rapid, and simple method of BrdU administration for SCE studies in vivo. BrdU was dissolved in melted agar and administered to mice as a single subcutaneous injection. Clear and reproducible sister chromatid differential staining of bone marrow cells was obtained. Melted agar, when injected into mice, solidified quickly and formed a clump which provided a sustained release of BrdU. HPLC analyses showed that a considerable serum level of BrdU was maintained for at least 10 h in a mouse injected with 25 mg of BrdU in agar.
D000362:Agar; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D001973:Bromodeoxyuridine / Q000097:blood / Q000494:pharmacology*; D002273:Carcinogens / Q000494:pharmacology; D002478:Cells, Cultured; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D002273:Carcinogens; D009153:Mutagens; D000362:Agar; D001973:Bromodeoxyuridine