Recovery of plant amines with carboxylic ion-exchange substrates and their subsequent analysis by thin-layer chromatography.
Two grades of carboxymethylcellulose (CMC) and five ion-exchange resins were compared for their effectiveness in recovering endogenous amines from plant extracts. If amine loss was to be limited to 10%, the H+ and Na+ forms of CMC could not be loaded with the solutes from more than 0.25 and 1.5 gram fresh weight (gfw) tissue per ml bed volume respectively. The corresponding ionic forms of a typical resin, Amberlite CG-50, would tolerate loadings ca. 5 X higher than this. However, it was then necessary to use very slow flow-rates (13 ml cm-2 h-1) for both applying and eluting the amines and, even so, they could not be quantitatively displaced from any resin tested, with the possible exception of Duolite C433. If the extract was acidic, maximum permissible loadings were reduced by a factor of 3 to 20, depending on substrate and ionic form. The composition of the amine fraction was essentially the same whatever substrate was used for its recovery and whatever percentage of it had been lost.
D000588:Amines / Q000032:analysis*; D002266:Carboxymethylcellulose Sodium; D002855:Chromatography, Thin Layer; D007475:Ion Exchange Resins; D010936:Plant Extracts / Q000032:analysis*
D000588:Amines; D007475:Ion Exchange Resins; D010936:Plant Extracts; D002266:Carboxymethylcellulose Sodium