Bovine skeletal muscle adenosine deaminase. Purification and some properties.
A low molecular weight form of adenosine deaminase from bovine skeletal muscle was purified about 930-fold. The enzyme had a mol. wt of 31,000, a Km value for adenosine of 2.37 X 10(-5) M and a pH optimum at 7.0. This enzyme is very resistant to heat inactivation and does not require metal activators or other dialysable cofactors. A possible role in the post-mortem metabolism of adenine nucleotide in skeletal muscle is discussed.
D000243:Adenosine Deaminase / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D002417:Cattle; D004355:Drug Stability; D007700:Kinetics; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D009700:Nucleoside Deaminases / Q000302:isolation & purification*; D013816:Thermodynamics
D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase