Chromatofocusing of apolipoproteins from human serum high density lipoprotein.
Human HDL was delipidated and the apolipoproteins were fractionated by chromatofocusing. Chromatofocusing, which separates proteins due to their differing isoelectric points, resulted in 8 peaks with corresponding pI values of 7.40, 6.92, 6.64, 5.48, 5.30, 5.18, 4.92 and 4.63. By one single chromatofocusing run four apolipoproteins were obtained in pure form. Two additional polypeptides could be purified during the desalting step using phenyl-Sepharose.
D001053:Apolipoproteins / Q000032:analysis*; D002847:Chromatography, Agarose; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D008075:Lipoproteins, HDL / Q000032:analysis / Q000097:blood*; D008297:Male
D001053:Apolipoproteins; D008075:Lipoproteins, HDL