Vinblastine-induced autophagocytosis: the effect of disorganization of microfilaments by cytochalasin B.
The effects of disorganization of cellular microfilaments by cytochalasin B on vinblastine-induced autophagocytosis was studied in Ehrlich ascites tumor cells in vitro. Incubation with vinblastine induced a formation of autophagic vacuoles in the cytoplasm. The disorganization of microfilaments by cytochalasin B failed to inhibit vinblastine-induced autophagocytosis. Incubation with cytochalasin B alone induced a rapid formation of blebs on the cell surface. These contained cytoplasmic organelles and were connected by a narrow shaft to the main part of the cell. Thin subcortical microfilaments seen in the control cell cytoplasm were apparently relocated after cytochalasin B treatment and formed amorphous masses deeper in the cytoplasm. Vinblastine did not affect the formation of blebs after cytochalasin B treatment.
D000818:Animals; D001343:Autophagy / Q000187:drug effects*; D002286:Carcinoma, Ehrlich Tumor; D003571:Cytochalasin B / Q000494:pharmacology*; D003599:Cytoskeleton / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D008870:Microtubules / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects*; D014747:Vinblastine / Q000494:pharmacology*
D003571:Cytochalasin B; D014747:Vinblastine