[Effect of cultivation conditions on the growth properties of Swiss 3T3 cells].
A possibility of using Swiss 3T3 cells, adapted to the growth in the Eagle basal medium and bovine serum, in studies of cell proliferation and quiescent state was shown on the basis of their growth characteristics. Proliferative activity of cultures was estimated by measuring the intensity of DNA synthesis (incorporation of labeled thymidine and flow cytofluorometric analysis), mitotic index and cell number counts. Growth rate and saturation density of the culture were analyzed depending on serum concentration, substrate quality and medium changes both in growth and quiescent states. In spite of repeated medium changes such adapted cells had saturation density within 4.10(4)--7.10(4) cells/cm2, standard for this line. Besides, a distinct inhibition of cell proliferation at confluence or after incubation with low serum (0.5%) and a possibility of the following stimulation of cell divisions by adding a fresh medium containing different concentrations of serum were demonstrated. The increased rate of adipose conversion was detected in resting confluent 3T3 cells cultivated in closed vessels, as compared to cells growing in tissue culture dishes in the CO2 incubator.
D000818:Animals; D002452:Cell Count; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured / Q000166:cytology*; D003470:Culture Media / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D051379:Mice; D008815:Mice, Inbred Strains; D008938:Mitosis; D013997:Time Factors
D003470:Culture Media; D004247:DNA
Vliianie usloviĭ kul'tivirovaniia na rostovye svoĭstva kletok Swiss 3T3.