title: Atrial natriuretic factor: atrial conversion of high to low molecular weight forms.
abstract: The atrial natriuretic factor elutes by gel filtration in high and low molecular weight fractions. Extraction and elution of rat atria in 1.0 M acetic acid yielded a predominance of the high molecular weight form(s); whereas when these procedures were carried out in 0.1 M acetic acid, there was a predominance of the low molecular weight forms. When partially purified high molecular weight natriuretic activity was eluted in 0.1 M acetic acid, the high molecular weight form(s) remained intact. When partially purified high molecular weight natriuretic activity was mixed with crude atrial extract in 0.1 M acetic acid, there was an apparent conversion to the low molecular weight forms. Extraction of rat atria in boiling 0.1 M acetic acid blocked this conversion. It is concluded that rat atria contain a heat labile factor that converts high molecular weight natriuretic activity to the low molecular weight forms.
mesh_terms: D000085:Acetates; D019342:Acetic Acid; D000818:Animals; D004355:Drug Stability; D006325:Heart Atria / Q000378:metabolism; D006358:Hot Temperature; D008297:Male; D008970:Molecular Weight; D009206:Myocardium / Q000378:metabolism*; D009318:Natriuresis; D045283:Natriuretic Agents; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000085:Acetates; D045283:Natriuretic Agents; D011506:Proteins; D019342:Acetic Acid