Properties of the lysosomes from liver and gill of rainbow trout, Salmo gairdnerii R.: effect of starvation, salinity and 2,4,5-T.
Three isolation procedures were used to test the labilization of the lysosomes after adaptation of the animals to seawater, starvation or acute and chronic treatments with 2,4,5-T, an organochlorine pesticide. The lysosomes from gill and liver had different properties with respect to their resistance to osmotic and mechanical shocks, or treatments with digitonin. Starvation induced a significant labilization of the lysosomes in liver, but not in gill. Salinity changes were without effect on the stability of the gill lysosomes, but induced an increase of the specific activity of the lysosomal enzyme beta-acetylglucosaminidase. Acute treatments with 2,4,5-T increased lysosomal fragility in the gill but not in the liver, while chronic treatments with this herbicide increased lysosomal lability in the two tissues. Liver lysosomes were much more susceptible to in vitro treatments with 2,4,5-T than gill lysosomes. The results are discussed with respect to the different functions of gill and liver, their exposure to the environment, and the possible discrimination between different lysosomal populations.
D015085:2,4,5-Trichlorophenoxyacetic Acid / Q000494:pharmacology*; D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D004072:Digitonin / Q000494:pharmacology; D005880:Gills / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure*; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure*; D009994:Osmolar Concentration; D013217:Starvation; D014337:Trout
D015085:2,4,5-Trichlorophenoxyacetic Acid; D000118:Acetylglucosaminidase; D004072:Digitonin