[Intravital selection of early mouse embryos by sex and karyotypic characteristics].
The possibility of live karyotyping by a single blastomere isolated at the 2-, 4-, and 8-cell stage has been investigated. It is expedient to use to this end a single blastomere isolated from a 4-cell embryo. Three rest blastomeres formed normal morulae or blastocysts upon cultivation during 44 or 68 hrs. When the isolated blastomeres were cultivated for 14-16 hrs in a medium 0.5 micrograms/ml colcemide, 97% of blastomeres were at the metaphase stage and 72% of chromosome plates were suitable for karyotyping. The prospects of the method proposed in experimental embryology and for selection of the early embryos of farm animals by sex are discussed.
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D001757:Blastomeres / Q000166:cytology; D002478:Cells, Cultured; D002970:Cleavage Stage, Ovum / Q000166:cytology*; D005260:Female; D006824:Hybridization, Genetic; D007621:Karyotyping; D008297:Male; D008677:Metaphase; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D012733:Sex Differentiation
Prizhiznennyĭ otbor rannikh zarodysheĭ mysheĭ po polu i osobennostiam kariotipa.