News about the neuronal membrane protein which binds the presynaptic neurotoxin beta-bungarotoxin.
beta-Bungarotoxin (beta-Btx) is cytotoxic for GABA-ergic and cholinergic neurons in chick retina explant cultures. Binding experiments with 125I-beta-Btx identified a high affinity binding site in membranes of chick brain. This binding is specific and its pharmacology indicates that it mediates the above-mentioned cytotoxicity. Photoaffinity crosslinking of 125I-beta-Btx to chick brain membranes showed that the beta-Btx binding protein contains a polypeptide of MW 95 000. The beta-Btx binding protein was solubilized with Triton X-100 and some of its biochemical and physical properties were characterized.
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D002038:Bungarotoxins / Q000633:toxicity; D002352:Carrier Proteins / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens; D008565:Membrane Proteins / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009924:Organ Culture Techniques; D011487:Protein Conformation; D012160:Retina / Q000187:drug effects
D002038:Bungarotoxins; D002352:Carrier Proteins; D008565:Membrane Proteins; C041451:beta-bungarotoxin binding protein, rat