The comparison of susceptibility of two target cells (HuRBC and L1210) to antibody dependent lymphocyte cytotoxicity.
Antibody-dependent lymphocyte cytotoxicity was compared in two test procedures. Human erythrocytes (group O R1R1 or R2R2) and mouse lymphoma cells (line L1210) were used as target cells. Anti-Rh (anti-C + D) serum obtained from a hyperimmunized blood donor and serum obtained from rabbit immunized with L1210 cells were used as the source of antibody specific for target cells. In both tests, lymphocytes (PBL) or mononuclear cells (MNC) isolated from heparinized or defibrinated blood were used as effectors. In both tests comparable results were obtained.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D004912:Erythrocytes / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007939:Leukemia L1210 / Q000276:immunology* / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice