Extraction of a synthetic androgen from fish muscle and quantitation by high performance liquid chromatography.
A rapid, reproducible extraction procedure was developed to recover the synthetic androgen 17 alpha-methyltestosterone (MT) from fish muscle. A single extraction with chloroform-methanol (2:1 V/V), followed by elution from mini-columns with various methanol concentrations, yielded an extraction efficiency of greater than 70%. Resolution of MT from testosterone was complete and reliable quantitation was achieved utilizing reversed-phase high performance liquid chromatography. Endogenous testosterone was not detectable in 1-g muscle samples, but MT was detected in fish muscle 6 h after adult Tilapia aurea were fed a diet containing the steroid.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005399:Fishes / Q000378:metabolism*; D008460:Meat / Q000032:analysis*; D008777:Methyltestosterone / Q000032:analysis* / Q000378:metabolism; D009132:Muscles / Q000032:analysis*; D013739:Testosterone / Q000032:analysis
D013739:Testosterone; D008777:Methyltestosterone