Growth characteristics of the yeast phase of Paracoccidioides brasiliensis in a chemically defined medium.
The growth of four clinical strains of Paracoccidioides brasiliensis was investigated using the chemically defined medium of McVeigh and Morton. Emphasis was placed upon controlling conditions of inoculum preparation, age of inoculum used, and the homogeneity of samples used for analysis. The medium was evaluated for its ability to support growth of the yeast phase of P. brasiliensis at 37 degrees C. Cultures were followed for 240 h and growth patterns were determined by measuring optical density, dry weight, nucleic acids and protein. The medium is excellent for growing the yeast phase of P. brasiliensis. The exponential phase lasted an average of 135 h and the stationary phase 72 h; a decline began after 207 h. This defined medium supports abundant growth of the yeast phase of P. brasiliensis and may thus prove useful in the preparation of yeast-phase antigens.
D003470:Culture Media; D004271:DNA, Fungal / Q000096:biosynthesis; D005656:Fungal Proteins / Q000096:biosynthesis; D005658:Fungi / Q000254:growth & development*; D006801:Humans; D010228:Paracoccidioides / Q000254:growth & development* / Q000378:metabolism; D012331:RNA, Fungal / Q000096:biosynthesis; D013997:Time Factors
D003470:Culture Media; D004271:DNA, Fungal; D005656:Fungal Proteins; D012331:RNA, Fungal