Serum indole-3-acetic acid in control subjects and newly abstinent alcoholics after an oral loading with L-tryptophan: a preliminary study using liquid chromatography with amperometric detection.
Described in this paper is a rapid, isocratic assay for serum indole-3-acetic acid (IAA). The sample preparation involves only protein precipitation using sulfosalicylic acid, and the sensitivity of amperometric detection is in the picogram range. The chromatographic analysis time is approximately 4 min. The devised method was used for a longitudinal study of IAA levels in serum samples from control subjects and newly abstinent alcoholics. Dietary variations were eliminated by administering a 2.0-g loading dose of L-Trp to all subjects investigated. The results are presented in the form of cumulative frequency polygons. Preliminary data indicate no differences in IAA levels between newly abstinent alcoholics and control subjects.
D000284:Administration, Oral; D000328:Adult; D000437:Alcoholism / Q000097:blood* / Q000534:rehabilitation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D006801:Humans; D007210:Indoleacetic Acids / Q000097:blood*; D008297:Male; D008832:Microchemistry; D013997:Time Factors; D014364:Tryptophan / Q000378:metabolism*
D007210:Indoleacetic Acids; C030737:indoleacetic acid; D014364:Tryptophan