The characterization of invertebrate troponin C.
TNCs from lobster, mussel, and squid migrated with rabbit TNC at an apparent mol. wt of 18,000. Electrophoretic mobilities in the presence or absence of Ca2+ were compared: the electrophoretic mobility of rabbit TNC was greater in the presence of Ca2+ than it its absence, but all invertebrate TNCs tested migrated identically, whether Ca2+ was present or not. The Ca2+-binding capacity of invertebrate TNCs was only one Ca2+ ion per molecule. The alpha-helix contents in the presence or absence of Ca2+ were compared: rabbit TNC changed by a value of 16% and invertebrate TNCs by 4%. Antibodies to loligo TNC did not cross-react with rabbit TNC, but did interact with their molluscan TNCs.
D000818:Animals; D049872:Bivalvia / Q000032:analysis; D002118:Calcium / Q000378:metabolism; D002942:Circular Dichroism; D049832:Decapodiformes / Q000032:analysis*; D009132:Muscles / Q000032:analysis; D008121:Nephropidae / Q000032:analysis*; D011485:Protein Binding; D011487:Protein Conformation; D011817:Rabbits; D013045:Species Specificity; D014336:Troponin / Q000302:isolation & purification* / Q000378:metabolism; D019209:Troponin C
D014336:Troponin; D019209:Troponin C; D002118:Calcium