The response of Slj/+ mice to experimental manipulation of the erythron.
We have compared the response of mice heterozygous for the Slj allele-which have a stromal defect resulting in mild macrocytic anemia-and their normal +/+ littermates to manipulations of the erythron to further characterize the effects of the Slj allele on hemopoiesis. CFU-S kinetics in Slj/+ mice following plethorization or induction of anemia did not differ from similarly treated +/+ littermates. Under all circumstances, however, Slj/+ mice had a smaller splenic CFU-S population than similarly treated +/+ mice. Slj/+ and +/+ mice responded similarly to induction of hemolytic anemia with a large rise in 59Fe incorporation in spleen and blood. Hypertransfused Slj/+ mice were shown to have a diminished response to exogenous erythropoietin in spleen and blood as compared with their +/+ littermates. We conclude that the regulation of CFU-S kinetics in mutant Slj/+ mice is not anomalous as has been reported to be the case in Sl/Sld mice. The defective stromal tissue of Slj/+ mice, however, does not support a normal-sized CFU-S population, which leads to decreased influx of progenitor cells into the erythropoietin-responsive cell compartment, causing the diminished response of plethorized Slj/+ mice to exogenous erythropoietin.
D000743:Anemia, Hemolytic / Q000139:chemically induced / Q000503:physiopathology; D000748:Anemia, Macrocytic / Q000235:genetics / Q000473:pathology / Q000503:physiopathology*; D000818:Animals; D001803:Blood Transfusion; D001815:Bloodletting; D001853:Bone Marrow / Q000473:pathology; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000473:pathology; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D010659:Phenylhydrazines; D011086:Polycythemia / Q000473:pathology; D013154:Spleen / Q000473:pathology
D010659:Phenylhydrazines; C030299:phenylhydrazine; D004921:Erythropoietin