Synthesis of oligonucleotides containing the hypermodified base, alpha-putrescinylthymine.
Dodecathymidylic acids containing alpha-putrescinylthymidine, 5-methylamino-methyl-2'-deoxyuridine and 5-methoxymethyl-2'-deoxyuridine were prepared by the phosphotriester polymer support synthesis. The melting temperatures of the synthetic oligomers-polydeoxyadenylic acid complexes show that the secondary amino group at putrescinylthymidine decreases the thermal stability of complexes and the primary amino group at the putrescinyl residue increases the stability.
D001483:Base Sequence; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis; D013329:Structure-Activity Relationship; D013941:Thymine / Q000031:analogs & derivatives*
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D011089:Polydeoxyribonucleotides; C025113:alpha-putrescinylthymine; D013941:Thymine