Utilization of murine peripheral blood lymphocytes for H-2 typing.
We adapted the NIH Standard Protocol for HLA-A, B, C typing to perform murine H-2 typing. The assay is direct, measuring the cytotoxicity of the antiserum/cell/complement reaction with a supravital dye. This method is advantageous because it: utilizes peripheral blood lymphocytes (PBL) obtained from the tail vein; uses microliter volumes of antiserum; is practical because the formalin fixed reactions need not be read immediately; involves standard and inexpensive cytotoxicity techniques; is easily interpreted and is readily reproducible.
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D006183:H-2 Antigens / Q000276:immunology*; D006650:Histocompatibility Testing / Q000379:methods*; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains
D006183:H-2 Antigens