Automated analysis of wide-field specular photomicrographs.
This report describes computerized image enhancement and analysis from the digitized image of a wide-field specular micrograph to produce an output of frequency distribution of cell areas. The steps involved including image smoothing and averaging, followed by automated scanning to detect individual cell boundaries, and cell area calculation. The limitations of this method include the requirement for expensive interactive display hardware and a 10% misinterpretation of cell boundaries by the algorithm.
D003201:Computers; D003315:Cornea / Q000166:cytology; D004727:Endothelium / Q000166:cytology; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D010784:Photomicrography / Q000295:instrumentation / Q000379:methods*