Theoretical and experimental approach to recognition of amino acid by tRNA and nucleotide II.
Strong and specific interactions between amino acids and their cognate tRNAs have been found by the CD, fluorescence, and UV absorption difference spectroscopy and by the gel-filtration method. New types of strong hydrogen bonding interactions between an amino acid and polynucleotides, nucleotides, nucleosides, and nucleic acid bases in aqueous solution have also been detected. These findings are strong experimental evidence for the C4N model of the genetic code. The cases of glutamate and aspartate will theoretically be discussed in details.
D000596:Amino Acids / Q000378:metabolism*; D000926:Anticodon; D002942:Circular Dichroism; D008957:Models, Genetic; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D046249:Transfer RNA Aminoacylation
D000596:Amino Acids; D000926:Anticodon; D009711:Nucleotides; D012343:RNA, Transfer