Suppression of sexual behavior and localization of [3H] puromycin after intracranial injection in the rat.
Bilateral intracranial injections of puromycin (10 micrograms/0.5 microliter vehicle) into the preoptic area (POA) of steroid-primed ovariectomized rats resulted in a significant decrease in the lordosis response when compared to saline treated controls. To determine the extent of puromycin diffusion after intracranial injection, [3H] puromycin was injected into the right POA of ovariectomized rats. Neural regions anterior, posterior and superior to the POA, and the right and left POA were assessed for tritium activity 0.5, 6 and 24 hours after stereotaxic surgery. The activity was primarily located at the injection site or in the injection tract. The highest amount of activity external to the injection site or injection tract was found in the right ventromedial hypothalamus.
D000818:Animals; D004965:Estrogen Antagonists / Q000502:physiology; D005260:Female; D009419:Nerve Tissue Proteins / Q000502:physiology; D011301:Preoptic Area / Q000187:drug effects* / Q000502:physiology; D011691:Puromycin / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012726:Sexual Behavior, Animal / Q000187:drug effects* / Q000502:physiology
D004965:Estrogen Antagonists; D009419:Nerve Tissue Proteins; D011691:Puromycin