Effect of estrogen on lysosomal enzyme activities in rat heart.
The activities per microgram DNA of five lysosomal enzymes [cathepsin D, cathepsin B, beta-N-acetylglucosaminidase (beta-NAG), beta-glucuronidase, and acid phosphatase] were measured in homogenates of female and male rat (Sprague-Dawley) hearts. Female rats were studied during stages of the estrous cycle and at 3 weeks after ovariectomy. Three-week-postovariectomized female rats and intact male rats were injected subcutaneously with 17 beta-estradiol-3-benzoate. Lysosomal enzyme activities in the male rat heart were more responsive to exogenous estradiol than were activities in the female rat heart. Cathepsin B, beta-NAG, and beta-glucuronidase were increased dramatically in the male rat heart upon short-term administration of estrogen (4 days). In both female and male rat hearts, activities of two lysosomal proteinases, cathepsins B and D, were reduced significantly (approximately 50%) by extended administration of estrogen for 10 days.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002369:Castration; D002401:Cathepsin B; D002402:Cathepsin D / Q000378:metabolism; D002403:Cathepsins / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005966:Glucuronidase / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D012737:Sex Factors
D004958:Estradiol; D000135:Acid Phosphatase; D005966:Glucuronidase; D000118:Acetylglucosaminidase; D002403:Cathepsins; D002401:Cathepsin B; D002402:Cathepsin D