The effects of X irradiation on the cytoskeleton of rat alveolar macrophages in vitro.
The three-dimensional visualization of Triton X-100 resistant cytoskeletons has been used to demonstrate that an absorbed dose of 120 Gy from X rays causes a distinctive and reproducible alteration of the cytoskeleton of intact rat alveolar macrophages in vitro. The alteration has also been shown to be rapidly and completely "repaired" and to be apparently similar to alterations caused by colchicine but dissimilar to those caused by cytochalasin B. From these observations and those of other workers who have studied the irradiation of extracted microtubular proteins in vitro, we think it likely that microtubules rather than microfilaments are the radiosensitive component of the macrophage cytoskeleton.
D000818:Animals; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000528:radiation effects* / Q000648:ultrastructure; D008264:Macrophages / Q000187:drug effects / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D011650:Pulmonary Alveoli / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains
D003571:Cytochalasin B; D011092:Polyethylene Glycols; D017830:Octoxynol; D003078:Colchicine