Double-axis rotary replication for deep-etching.
We have developed a simple new method of rotary shadowing, double-axis rotary replication, for observing three-dimensional structures in deep-etched, rapid-frozen tissues. The technical details of this method are described and compared with the conventional fixed-angle rotary shadowing procedure.
D000818:Animals; D005613:Freeze Etching / Q000379:methods*; D006168:Guinea Pigs; D007089:Image Enhancement / Q000379:methods*; D051381:Rats; D012399:Rotation