Phospholipase A2 activity in rat and human lymphocytes.
Partial characterization of phospholipase A from rat and human lymphocytes showed that it was much less active in man than in rat. The use of phosphatidylethanolamine labelled in the 2 position as substrate established that phospholipase A activity was 2 acyl-specific. It was maximal at pH 7.0 to 8.0, totally Ca2+ dependent and inhibited by detergents and Indomethacin.
D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007213:Indomethacin / Q000494:pharmacology; D007700:Kinetics; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D010740:Phospholipases / Q000097:blood*; D010741:Phospholipases A / Q000097:blood*; D054467:Phospholipases A2; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D002122:Calcium Chloride; D007213:Indomethacin