A sensitive double isotopic, chromatographic method for the determination of histamine. I: Studies with authentic histamine.
This paper presents a method for the determination of histamine using an internal standard of 14C-labelled histamine, which together with an unknown sample of unlabelled histamine is dansylated with 3H-labelled dansyl chloride. The products of this dansylation are then separated on thin-layer-chromatographic plates coated with micropolyamide. The spot of dansylhistamine is scraped off and the content of histamine in the unknown sample is calculated from the 3H/14C-ratio in the spot.
D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D003619:Dansyl Compounds; D006632:Histamine / Q000032:analysis*
D003619:Dansyl Compounds; D006632:Histamine; C029038:dansyl chloride