Ultraviolet carcinogenesis in the hairless mouse skin. Influence of the sunscreen 2-ethylhexyl-p-methoxycinnamate.
The mutagenicity of some samples of a commonly used sunscreen, 2-ethylhexyl-p-methoxycinnamate (2-EHMC), led to these studies of its potential carcinogenicity in the HRA/Skh hairless mouse. In a daily treatment regime, repeated for 9 weeks, groups of mice were painted on the dorsum with 2-EHMC, and were then exposed to low doses of one of two artificial ultraviolet (UV) light sources. Mice were also treated with UV alone and with 2-EHMC alone. The accumulated UV exposure alone produced tumours in 40-100% of mice. However, 2-EHMC-treated mice were protected. Subsequent treatment of the 2-EHMC-protected mice, and mice previously treated with 2-EHMC alone, with the tumour promoter, croton oil, produced tumours on a significant number of animals. We conclude that 2-EHMC protects from UV tumorigenesis in the absence of a tumour promoter. However, although tumours appeared on only 4 out of 160 2-EHMC-treated mice exposed to UV, the carcinogenic process had been initiated in others, as application of the tumour promoter, croton oil, produced tumours. Statistical analysis of the incidence of promoted tumours inferred that prior irradiation with UV may not have been implicated. Therefore, 2-EHMC itself may initiate tumours in this strain of hairless mouse.
D000818:Animals; D002934:Cinnamates / Q000009:adverse effects*; D003043:Cocarcinogenesis; D003436:Croton Oil; D051379:Mice; D008812:Mice, Hairless; D009152:Mutagenicity Tests; D009381:Neoplasms, Radiation-Induced / Q000473:pathology; D012878:Skin Neoplasms / Q000139:chemically induced / Q000209:etiology* / Q000473:pathology; D013473:Sunscreening Agents / Q000009:adverse effects*; D014466:Ultraviolet Rays
D002934:Cinnamates; D013473:Sunscreening Agents; C118580:octylmethoxycinnamate; D003436:Croton Oil