Differential biosynthesis of proteins in separated gastric mucosal cells.
Rat gastric mucosal cells were separated according to size by rate-zonal sedimentation through a medium containing a gradient of bovine albumin. Numbers and mean sizes of cells indicated two main populations, 430-1000 microns 3 and 1500-2500 microns 3, with a discontinuity between 1000 and 1500 microns 3. Gradient fractions, combined into pools of increasing cell size, synthesized radiolabelled proteins which were resolved by electrophoresis, densitometry and computation. The smallest cells (430-800 microns 3) tended to synthesize radiolabelled proteins with the highest relative specific radioactivities. The decrease in relative specific radioactivity with increase in cell size was compatible with a progression from precursor cells towards the more differentiated state.
D000818:Animals; D002250:Carbon Radioisotopes; D002469:Cell Separation; D002499:Centrifugation, Density Gradient / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism*; D066298:In Vitro Techniques; D008970:Molecular Weight; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains
D002250:Carbon Radioisotopes; D011506:Proteins