The determination of flux through phenylalanine hydroxylase and homogentisate oxidase in isolated hepatocytes.
The metabolic fate of [4-3H]phenylalanine incubated with isolated hepatocytes has been investigated. Analysis of 3H2O production and fluorimetric estimation of tyrosine production has allowed calculation of flux through phenylalanine hydroxylase and homogentisate oxidase. The resulting flux determinations are physiologically relevant and hormonally sensitive.
D000818:Animals; D002478:Cells, Cultured; D049308:Dioxygenases; D050560:Homogentisate 1,2-Dioxygenase; D006713:Homogentisic Acid / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D010105:Oxygenases / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism*; D010651:Phenylalanine Hydroxylase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014443:Tyrosine / Q000096:biosynthesis
D014443:Tyrosine; D010649:Phenylalanine; D010105:Oxygenases; D049308:Dioxygenases; D050560:Homogentisate 1,2-Dioxygenase; D010651:Phenylalanine Hydroxylase; D006713:Homogentisic Acid