Rapid and sensitive determination of acetylsalicylic acid and its metabolites using reversed-phase high-performance liquid chromatography.
A rapid and sensitive high-performance liquid chromatographic technique was developed for the simultaneous determination of gentisic acid, salicyluric acid, acetylsalicylic acid and salicylic acid in plasma and serum. The method involved a single deproteinization step and separation using a reversed-phase column eluted with a buffered methanol (35%) mobile phase. Detection was achieved with a variable-wavelength ultraviolet detector set at 235 nm and a given chromatographic analysis could be completed in less than 10 min. The method was tested in both human and animal (rat) models given a single dose of acetylsalicylic acid.
D000818:Animals; D001241:Aspirin / Q000097:blood*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005841:Gentisates / Q000097:blood; D006626:Hippurates / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051381:Rats; D012459:Salicylates / Q000097:blood; D020156:Salicylic Acid; D013045:Species Specificity
D005841:Gentisates; D006626:Hippurates; D012459:Salicylates; C000693:salicylurate; D020156:Salicylic Acid; D001241:Aspirin