title: Detection of reaction products of thiamin degradation by catechol derivatives.
abstract: To clarify the mechanism of thiamin degradation by catechol derivatives, thiamin and its analogues were incubated with caffeic acid or catechol in 1/15 M phosphate buffer pH 7.4 at 48 degrees C or 50 degrees C. Using thin-layer chromatography and high-performance liquid chromatography, several unknown intermediates of the reaction product, which may have both pyrimidine and catechol moieties, were detected.
mesh_terms: D002109:Caffeic Acids; D002396:Catechols / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D013831:Thiamine / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D002109:Caffeic Acids; D002396:Catechols; C040048:caffeic acid; C013293:thiamine disulfide; D013831:Thiamine