Assignment of acyl chain resonances from membranes of mammalian cells by two-dimensional NMR methods.
Two-dimensional nuclear magnetic resonance (NMR) methods have been successfully used to assign resonances in the 1H NMR spectrum of intact viable rat mammary adenocarcinoma cells. Two-dimensional scalar-correlated spectroscopy identifies connectivities for resonances of the lipid acyl chains in the plasma membrane of these cells. We expect that two-dimensional scalar-correlated methods may be of general use for providing unequivocal assignments in the complex and often poorly resolved 1H NMR spectra of cells.
D000230:Adenocarcinoma; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane; D005260:Female; D009682:Magnetic Resonance Spectroscopy; D008325:Mammary Neoplasms, Experimental; D008563:Membrane Lipids; D051381:Rats
D008563:Membrane Lipids