[Detection and isolation of glycoproteins and nuclear proteins in the bovine brain].
Using ammonium sulphate precipitation, ion exchange chromatography and preparative isoelectrofocusing, 9 organ-specific glycoproteins and 16 specific nuclear proteins were isolated from bovine brain nervous tissue in a homogeneous state. The isolation of proteins was controlled by a solid phase immunoenzymatic analysis. The molecular weight, subunit composition and isoelectric points of the proteins were determined and their ability to interact with immobilized calf thymus DNA and concanavalin A was demonstrated. It was assumed that the multiplicity of specific proteins of brain tissue is a molecular basis which provides for the functional specificity of the nervous tissue at large.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002852:Chromatography, Ion Exchange; D006023:Glycoproteins / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D009698:Nucleoproteins / Q000302:isolation & purification*; D009928:Organ Specificity; D011817:Rabbits; D051381:Rats; D013950:Thymus Gland / Q000032:analysis
D006023:Glycoproteins; D009419:Nerve Tissue Proteins; D009698:Nucleoproteins; C020983:brain glycoprotein NSA 3
Vyiavlenie i vydelenie glikoproteinov i iadernykh belkov mozga byka.