Transport of branched-chain amino acids in brain slices of developing and adult rats.
The accumulation of labelled leucine, isoleucine and valine by cerebral slices of developing and adult rats was studied. The accumulation increased with age by 15-25%. It was strongly (from 52 to 86%) inhibited by a 100-fold excess of phenylalanine, tryptophan and another branched-chain amino acid, but moderately activated by GABA and glutamate. The inhibitions evoked by leucine and isoleucine were slightly stronger in young than in adult rats. The corresponding 2-oxoacid analogs of leucine, isoleucine and valine were also inhibitory but less effective. The 30-min accumulation of 3H-labelled branched-chain amino acids was ostensibly higher than the increase in their total concentrations in incubated slices, which apparently bespeaks lively homoexchange of endogenous intracellular and labelled extracellular amino acids.
D000375:Aging; D000596:Amino Acids / Q000494:pharmacology; D000597:Amino Acids, Branched-Chain / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D001921:Brain / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D066298:In Vitro Techniques; D007532:Isoleucine / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014633:Valine / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D000596:Amino Acids; D000597:Amino Acids, Branched-Chain; D007532:Isoleucine; D005680:gamma-Aminobutyric Acid; D007930:Leucine; D014633:Valine