title: C4 polymorphism in the dog: molecular heterogeneity of the C4 alpha and C4 gamma subunit chains.
abstract: Using an immunoblotting technique and goat antihuman C4, we observed five distinct electrophoretic variants of C4 in a panel of 60 random dogs. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of immunoprecipitated C4 showed that dog C4 is composed of three polypeptide subunit chains (alpha, beta, and gamma) and that structural variability occurs within the alpha- and gamma-chain regions. Two distinct molecular weight forms of both the C4 alpha- (alpha A and alpha B) and C4 gamma- (gamma A and gamma B) chain were detected. The variant forms of C4 alpha and C4 gamma were found in association with particular C4 allotypes.
mesh_terms: D000818:Animals; D003181:Complement C4 / Q000235:genetics*; D004285:Dogs / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D046911:Macromolecular Substances; D010375:Pedigree; D011110:Polymorphism, Genetic
chemical_list: D003181:Complement C4; D046911:Macromolecular Substances