Effects of methotrexate, ampicillin and gentamicin alone and in combination on the in vitro locomotion on human polymorphonuclear cells (PMN).
The effect of ampicillin 25 mg/ml, gentamicin 5 mg/l and methotrexate 10 & 70 mg/l, alone and in combination, was tested for their influence on human polymorphonuclear cell-locomotion, using zymosan or a bacterial filtrate of E. coli, with or without 0.25 and 0.025 mg/l of ampicillin and gentamicin, respectively, as attractants. Methotrexate and gentamicin alone decreased the locomotion, using zymosan as attractant. No effect was observed using the pure bacterial filtrate. The filtrate containing antibiotics displayed a lower ability to induce locomotion when the leucocytes had been pre-treated with antibiotics alone or in combination with methotrexate but not with methotrexate alone. Aspects of these findings are discussed.
D000667:Ampicillin / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004338:Drug Combinations; D005839:Gentamicins / Q000494:pharmacology*; D006098:Granulocytes / Q000187:drug effects*; D006801:Humans; D008727:Methotrexate / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology
D004338:Drug Combinations; D005839:Gentamicins; D000667:Ampicillin; D008727:Methotrexate