Cyanogenic glycosides and cyanohydrins in plant tissues. Qualitative and quantitative determination by enzymatic post-column cleavage and electrochemical detection, after separation by high-performance liquid chromatography.
A rapid, simple and reproducible high-performance liquid chromatography procedure is described for the qualitative and quantitative analysis of mixtures of cyanogenic glycosides. The separation is achieved by means of a reversed-phase (C8) column eluted with a phosphate buffer, pH 5.0, containing either 15 or 7.5% (v/v) methanol, 7.5% being necessary for resolution of epimeric pairs of the more hydrophilic glycosides. When this separation is combined with enzymatic post-column cleavage and electrochemical detection of the cyanide formed, a highly specific and very sensitive system is obtained. The method was applied to cyanogenic glycosides in crude plant tissue extracts, and compared with both a thin-layer chromatographic method and to a traditional determination of total cyanide released after hydrolysis. Sensitivity, selectivity and accuracy were found sufficient to enable its routine use for analysis of food and fodder samples, for example. Cyanohydrins could be detected qualitatively.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D006027:Glycosides / Q000032:analysis*; D009570:Nitriles / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D013237:Stereoisomerism
D006027:Glycosides; D009570:Nitriles; D010936:Plant Extracts; C007173:cyanogenic glycosides