Cytotoxin and enterotoxin production by Clostridium difficile.
30 strains of Cl. difficile isolated from faeces of patients with pseudomembranous colitis (PMC), antibiotic associated diarrhoea (AAC) and other intestinal disorders and from faeces of asymptomatic carriers were studied for production of toxins. Tissue culture assay was used for the detection of cytotoxin (toxin B) and ileal loop test for enterotoxin (toxin A). All Cl. difficile isolates from patients with PMC and AAC were found to produce cytotoxin, whereas enterotoxin was demonstrated only in approximately 70% of strains.
D000818:Animals; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002353:Carrier State / Q000382:microbiology; D003013:Clostridium / Q000378:metabolism*; D003015:Clostridium Infections / Q000382:microbiology; D003603:Cytotoxins / Q000096:biosynthesis*; D003967:Diarrhea / Q000382:microbiology; D004761:Enterocolitis, Pseudomembranous / Q000382:microbiology; D004768:Enterotoxins / Q000096:biosynthesis*; D005243:Feces / Q000382:microbiology; D006801:Humans; D007410:Intestinal Diseases / Q000382:microbiology; D011817:Rabbits
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003603:Cytotoxins; D004768:Enterotoxins; C031815:toxB protein, Clostridium difficile