Stimulatory effect of polyprenoic acid E5166 on 2-deoxy-D-glucose uptake by Swiss 3T3 cells.
3,7,11,15-Tetramethyl-2,4,6,10,14-hexadecapentaenoic acid (E5166), a synthetic analog of retinoic acid, increased 2-deoxy-D-glucose (2DG) uptake in a dose and time-dependent manner in mouse fibroblasts, Swiss 3T3 cells. Kinetic analysis of 2DG uptake showed that the Vmax for 2DG uptake by E5166-treated cells was greater than that of the control, while the Km values were essentially the same. Most of the E5166-stimulated 2DG uptake was suppressed by cycloheximide, although part of the stimulation always remained despite of the cycloheximide treatment. These results suggest that E5166-induced stimulation of 2DG uptake is due to the increase in the synthesis and recycle of hexose transporters (carriers) in Swiss 3T3 cells.
D000818:Animals; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D014176:Protein Biosynthesis; D013268:Stimulation, Chemical; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000494:pharmacology
D003837:Deoxy Sugars; D003571:Cytochalasin B; D014212:Tretinoin; C034534:3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid; D003513:Cycloheximide; D003847:Deoxyglucose