The use of liquid chromatography with dual-electrode electrochemical detection in the investigation of glutathione oxidation during benzene metabolism.
Liquid chromatography with electrochemical detection provides a powerful tool for the study of glutathione oxidation during benzene metabolism. The use of two dual-electrode detectors allows for the detection of oxidized and reduced glutathione and of phenol and quinone metabolites of benzene. The role of glutathione as a reductant is explored in this paper. Results indicate that hydrogen peroxide is the oxidizing agent primarily responsible for glutathione oxidation during benzene, phenol and hydroquinone metabolism.
D000818:Animals; D001554:Benzene / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods; D004563:Electrochemistry; D005978:Glutathione / Q000378:metabolism*; D006873:Hydroquinones / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D011506:Proteins / Q000378:metabolism
D006873:Hydroquinones; D011506:Proteins; D009249:NADP; D005978:Glutathione; D001554:Benzene; C031927:hydroquinone