High-performance liquid chromatographic method for screening disorders of aromatic acid metabolism using a multi-detection system.
This paper describes the use of a high-performance liquid chromatograph equipped with an ultraviolet multi-detection system for the analysis of aromatic acids to help establish a high-risk screening system for disorders of organic acid metabolism. The peak height ratios of about seventy metabolically important aromatic acids have been compiled using the multi-detection system. It may be possible to identify aromatic acids by comparing retention time and peak height ratios. The method was very effective for the diagnosis of disorders of aromatic acid metabolism.
D001565:Benzoates / Q000378:metabolism; D019817:Benzoic Acid; D002264:Carboxylic Acids / Q000378:metabolism*; D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003404:Creatinine / Q000652:urine; D006801:Humans; D007202:Indicators and Reagents; D008661:Metabolism, Inborn Errors / Q000175:diagnosis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D001565:Benzoates; D002264:Carboxylic Acids; D007202:Indicators and Reagents; D019817:Benzoic Acid; D003404:Creatinine