[Changes in mitochondrial lipid composition in hypoxia in the presence of a dinitrophenol uncoupler].
Incubation of mitochondria under hypoxic conditions in hypotonic solution of sucrose containing dinitrophenol as an uncoupler of oxidative phosphorylation led to hydrolysis of mitochondrial phospholipids, with the most pronounced destruction of phosphatidyl ethanolamine and cardiolipin fractions. Hydrolysis of diacyl phospholipids was accompanied by an increase in content of the corresponding lyso-derivatives - lysophosphatidyl ethanolamine, lysocardiolipin. This phenomenon was not observed after addition of tetracaine hydrochloride, inhibitor of phospholipases, suggesting that mitochondrial phospholipases are activated under hypoxic conditions in presence of dinitrophenol.
D000818:Animals; D004140:Dinitrophenols / Q000494:pharmacology*; D066298:In Vitro Techniques; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010100:Oxygen; D010740:Phospholipases / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D013748:Tetracaine / Q000494:pharmacology; D014475:Uncoupling Agents / Q000494:pharmacology*
D004140:Dinitrophenols; D010743:Phospholipids; D014475:Uncoupling Agents; D013748:Tetracaine; D010740:Phospholipases; D010100:Oxygen
Izmenenie lipidnogo sostava mitokhondriĭ pri gipoksii v prisutstvii razobshchitelia dinitrofenola.