title: A monoclonal antibody specific for the carboxy-terminus of angiotensin II.
abstract: A monoclonal antibody specific for the carboxy-terminus of angiotensin II was produced from the somatic cell fusion between SP2/0 myeloma cells and spleen cells from CAF/J mice immunized with angiotensin II selectively coupled to thyroglobulin. The subcloned line D5-7R2 was augmented in the ascites form and shown to be specific for angiotensin II (octapeptide) and shorter angiotensin peptides. This antibody, however, did not cross-react with angiotensin I (decapeptide). This selectivity will be important in clinical assays that measure angiotensin II in the presence of angiotensin I.
mesh_terms: D000804:Angiotensin II / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D003429:Cross Reactions; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; D000804:Angiotensin II