Actin purification from a gel of rat brain extracts.
Actin, 99% pure, has been recovered from rat brain with a high yield (greater than 15 mg/100 g brain). We have shown that: 1. a low ionic strength extract from rat brain tissue is capable of giving rise to a gel; 2. actin is the main gel component and its proportion is one order of magnitude higher than in the original extract; 3. actin can be isolated from this extract by a three-step procedure involving gelation, dissociation of the gel in 0.6 M KCl, followed by one or two depolymerization-polymerization cycles.
D000199:Actins / Q000302:isolation & purification*; D000818:Animals; D001923:Brain Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005782:Gels; D007700:Kinetics; D008722:Methods; D008854:Microscopy, Electron; D009132:Muscles / Q000032:analysis; D011108:Polymers / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
D000199:Actins; D005782:Gels; D011108:Polymers