[Uptake of 67Ga into the rat liver after treatment with thioacetamide].
67Ga uptake of the liver began to elevate from the 1st day and reached a maximum at the 2nd day of treatment with thioacetamide (TIAA). Incorporation of 3H-thymidine into the liver DNA fraction was reached a maximum at the 1.5th day, and the value was 5.7 times of the control. The uronic acid content and 35S incorporation in the 1.2 M NaCl-soluble fraction which contained predominantly heparan sulfate (HS), were both peaked at the 2nd day. These patterns were in good agreement with that of 67Ga uptakes in the liver treated with TIAA. Pretreatment of aminoacetonitrile, an inhibitor of fibrosis, was effective in lowering the elevated uptake of 67Ga in TIAA-treated rat liver. Uptake of the 67Ga in the TIAA-treated liver was also inhibited when they were treated with cycloheximide, an inhibitor of protein synthesis. On the other hand no significant inhibition was observed in the cytosine arabinoside-treated-TIAA rats. These results suggest that HS may be involved in the 67Ga uptake in damaged liver, and that relation between 67Ga uptake and cell proliferation is secondary.
D000081:Acetamides / Q000633:toxicity*; D000818:Animals; D002454:Cell Differentiation; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D003561:Cytarabine / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D005710:Gallium Radioisotopes / Q000378:metabolism; D006497:Heparan Sulfate / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D013853:Thioacetamide / Q000633:toxicity*; D013936:Thymidine / Q000378:metabolism
D000081:Acetamides; D003513:Cycloheximide; D003561:Cytarabine; D004247:DNA; D005710:Gallium Radioisotopes; D006497:Heparan Sulfate; D013853:Thioacetamide; D013936:Thymidine