Studies on the mechanism of inhibition of amphibian oocyte adenylate cyclase by progesterone.
Progesterone treatment induces the meiotic maturation of Xenopus laevis oocytes. Previous evidence indicates that this hormonal effect may be due to inhibition of oocyte adenylate cyclase. The present work studies several aspects of the mechanism of adenylate cyclase inhibition by this hormone. Forskolin greatly stimulates oocyte adenylate cyclase in the absence of guanine nucleotides and this activity is not sensitive to progesterone inhibition. In addition the forskolin-activated enzyme is not inhibited by a wide range of guanine nucleotide, in the presence or absence of hormone. The time course of cAMP synthesis catalyzed by oocyte adenylate cyclase in the presence of guanyl-5'l-imidodiphosphate (Gpp(NH)p) shows an initial lag period that does not depend on the concentration of Gpp(NH)p. Progesterone causes a very significant increase in the hysteresis of the reaction, at least doubling the half-time of enzyme activation. The hormonal effect on the lag cannot be reversed by saturating concentrations of Gpp(NH)p. Progesterone also decreases the steady-state rates of the reaction. This effect, however, depends on the concentration of Gpp(NH)p. High concentrations of Gpp(NH)p almost completely reverse the inhibition of the steady-state rates. Progesterone does not inhibit if it is added to the reaction after the initial lag period. Guanosine-5'-O-(2-thiodiphosphate) (GDP-beta-S) is an efficient competitive inhibitor of Gpp(NH)p activation of adenylate cyclase. Progesterone inhibition is observed at all concentrations of GDP-beta-S and is potentiated at high ratios of GDP-beta-S to Gpp(NH)p. These data indicate that progesterone inhibits by interfering with the activation of the Ns subunit of the enzyme by guanine nucleotides, rather than through a mechanism involving a separate Ni subunit.
D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D005576:Colforsin; D004224:Diterpenes / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006153:Guanosine Diphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D009865:Oocytes / Q000201:enzymology*; D011374:Progesterone / Q000494:pharmacology*; D013873:Thionucleotides / Q000494:pharmacology; D014982:Xenopus laevis
D000067956:Adenylyl Cyclase Inhibitors; D004224:Diterpenes; D013873:Thionucleotides; D006153:Guanosine Diphosphate; D005576:Colforsin; D006165:Guanylyl Imidodiphosphate; D011374:Progesterone; C023427:guanosine 5'-O-(2-thiodiphosphate)