[45Ca2+ uptake by rat thymocytes in the presence of blood serum of patients with cystic fibrosis].
The results of examination of 45Ca2+ (uptake by freshly prepared) thymocytes from rat in the presence of blood serum from CF patients and healthy persons as differentiating agent are presented. The presence of the factor responsible for enhanced Ca uptake in the blood serum of CF patients was found in our experimental system. That factor was absent in the blood serum of rats chronically treated with reserpine. This is in agreement with previous findings that this factor was related to the basic genetic defect in CF(6). Very efficient influx of 45Ca2+ into the rat thymocytes (up to 6,2%) being accompanied by increased calcium uptake in the presence of serum factor of CF patients in comparison with control serum of healthy persons should be underlined.
D000818:Animals; D002132:Calcium Radioisotopes / Q000378:metabolism*; D003470:Culture Media; D003550:Cystic Fibrosis / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism*
D002132:Calcium Radioisotopes; D003470:Culture Media
Wychwytywanie 45CA2+ przez tymocyty szczura w obecności surowicy krwichorych na zwłóknienie torbielowate (CF).