Crosslinking of tropomyosin to myosin subfragment-1 in reconstituted rabbit skeletal thin filaments.
Rabbit skeletal tropomyosin was labeled with the bifunctional photoactivatable crosslinker N-succinimidyl-6- (4'-azido-2'-nitrophenylamino)hexanoate. After irradiating the rigor complex composed of myosin subfragment-1, crosslinker-labeled tropomyosin, and F-actin, a crosslinked product was formed. This product was identified as a 1:1 adduct of tropomyosin and subfragment-1. This finding is in support of recent structural studies which suggest that tropomyosin and subfragment-1 are in close proximity to each other, and may be relevant to the mechanism of thin filament regulation.
D000199:Actins / Q000378:metabolism; D000818:Animals; D001386:Azides / Q000378:metabolism*; D002250:Carbon Radioisotopes; D003432:Cross-Linking Reagents / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D015879:Myosin Subfragments; D009218:Myosins / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D011817:Rabbits; D014316:Tritium; D014335:Tropomyosin / Q000378:metabolism*
D000199:Actins; D001386:Azides; D002250:Carbon Radioisotopes; D003432:Cross-Linking Reagents; D015879:Myosin Subfragments; D010446:Peptide Fragments; D014335:Tropomyosin; D014316:Tritium; C037733:N-succinimidyl-6-(4'-azido-2'-nitrophenylamino)hexanoate; D009218:Myosins