[Complex study of the oxidative metabolism of peritoneal macrophages attached to glass].
A method of obtaining a peritoneal macrophage enriched population on a slide in a special chamber has been worked out and tested. It has been shown that such cells keep specific morphological and functional peculiarities, including the ability to phagocytosis. They are in active state and can be utilized for studying the biological oxidation processes. A relative activity of oxidative metabolism of key enzymes has been evaluated. The possibility of studying the respiratory chain activity in these cells was shown by polarographic and fluorescent methods. Alternative oxygen-dependent systems were investigated. It was determined that NADPH-oxidase of peritoneal macrophages attached to a slide, responsible for "the respiratory burst" formation and defining their bactericidal properties and peroxide generating ability, is in the active state.
D000818:Animals; D001202:Ascitic Fluid / Q000378:metabolism* / Q000473:pathology; D002448:Cell Adhesion; D005898:Glass; D006651:Histocytochemistry; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D010587:Phagocytosis; D051381:Rats; D013211:Staphylococcus aureus
Kompleksnoe izuchenie okislitel'nogo metabolizma peritoneal'nykh makrofagov, prikreplennykh k steklu.