Electrochemical detection of cyanogenic glycosides after enzymatic post-column cleavage.
Crude and partly purified extracts from Helix pomatia and linamarase from cassava were immobilized on columns packed with porous glass or silica and used as post-column reactors in the high-performance liquid chromatography of cyanogenic glycosides. Sodium hydroxide (2 M) was added to the flowstream after the enzyme-reactor resulting in the formation of cyanide, which was then detected at a silver electrode by an amperometric measurement at 0 V with reference to a silver-silver chloride electrode. The selective detection of cyanide allows measurements in a complex matrix. The response is linear and the detection limit is in the low picomole range.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003486:Cyanides / Q000032:analysis*; D004563:Electrochemistry; D006026:Glycoside Hydrolases / Q000032:analysis; D006027:Glycosides / Q000032:analysis*; D006372:Helix, Snails / Q000378:metabolism; D006801:Humans; D009570:Nitriles / Q000652:urine; D010936:Plant Extracts / Q000032:analysis*; D012834:Silver
D003486:Cyanides; D006027:Glycosides; D009570:Nitriles; D010936:Plant Extracts; C007173:cyanogenic glycosides; D012834:Silver; D006026:Glycoside Hydrolases; C005091:linamarin