Effects of local anesthetics on phorbol ester-induced ornithine decarboxylase activity in mouse and human skin.
In preparation for experiments to determine the effects of various orally administered compounds on human skin ornithine decarboxylase (ODC) activity, it was observed that intradermal lidocaine hydrochloride inhibited 12-0-tetradecanoylphorbol-13-acetate (TPA)-induced ODC. Topically applied ethyl chloride had variable and unpredictable effects on ODC induction. Adequate local anesthesia can be obtained by using intradermal 1.0% procaine hydrochloride or normal saline; neither of these agents inhibited TPA induction of epidermal ODC activity in incubated human skin punch biopsies.
D000779:Anesthetics, Local / Q000494:pharmacology*; D000818:Animals; D001706:Biopsy / Q000379:methods; D004311:Double-Blind Method; D004790:Enzyme Induction / Q000187:drug effects; D005018:Ethyl Chloride / Q000494:pharmacology; D005260:Female; D006801:Humans; D008012:Lidocaine / Q000494:pharmacology; D051379:Mice; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D010703:Phorbol Esters / Q000037:antagonists & inhibitors*; D010704:Phorbols / Q000037:antagonists & inhibitors*; D011343:Procaine / Q000494:pharmacology; D011897:Random Allocation; D012867:Skin / Q000187:drug effects* / Q000201:enzymology; D012965:Sodium Chloride / Q000494:pharmacology; D013048:Specimen Handling
D000779:Anesthetics, Local; D010703:Phorbol Esters; D010704:Phorbols; D012965:Sodium Chloride; D005018:Ethyl Chloride; D011343:Procaine; D008012:Lidocaine; D009955:Ornithine Decarboxylase