Fibrinolytic activity in healthy volunteers before and after 5 to 20 minutes of venous occlusion.
We have determined in 22 volunteers the fibrinolytic activities of regular and of dextran sulfate precipitated euglobulin fractions in the presence and absence of flufenamate before and after 5 to 20 min of venous occlusion. Using the fibrin plate assay, Hageman factor Independent Intrinsic Fibrinolytic activity ( HIIF ) was measured in plasma euglobulins obtained from resting subjects in the morning. Hageman factor Dependent Intrinsic Fibrinolysis ( HDIF ) was calculated by subtracting the HIIF from the activity obtained after activation of the "resting plasma" by dextran sulfate. The activity of vascular Plasminogen Activator (v-PA) released after venous stasis was determined by subtraction of resting HIIF from post-occlusion total plasminogen activator activity. In normal subjects, the distribution of all activities followed a logarithmic scale. Twelve of 22 volunteers exhibited maximal v-PA activity after 20 min of venous occlusion. In three, it was maximal after 5 min, in three others after 10 min, and in four after 15 min of venous stasis. These results demonstrates that it may not be possible to clearly distinguish normal from abnormal responders using a stasis test of fixed duration.
D000293:Adolescent; D000328:Adult; D001775:Blood Circulation; D003250:Constriction; D005260:Female; D005342:Fibrinolysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010960:Plasminogen Activators / Q000378:metabolism; D012712:Serum Globulins / Q000378:metabolism; D013997:Time Factors; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism
D012712:Serum Globulins; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator