Induction of immunoglobulin gene expression in mouse fibroblasts by cycloheximide treatment.
A complete set of a rearranged human gamma 1-heavy chain gene, HIG1, was cloned from human plasma cell leukemia line, ARH-77, and transferred into mouse cells. It was strongly expressed in mouse myeloma cells but not in mouse L cells, indicating that immunoglobulin gene expression is not species-specific but cell-specific. However, a remarkable production of human gamma 1 chain was induced in mouse L cells containing HIG1 gene when the cells were treated with cycloheximide for a short period. The role of a labile repressor molecule in the expression of the immunoglobulin gene is proposed.
D000818:Animals; D001483:Base Sequence; D003513:Cycloheximide / Q000494:pharmacology*; D004247:DNA / Q000032:analysis; D005347:Fibroblasts / Q000187:drug effects / Q000276:immunology; D005786:Gene Expression Regulation / Q000187:drug effects*; D007136:Immunoglobulins / Q000235:genetics*; D051379:Mice; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis
D007136:Immunoglobulins; D012333:RNA, Messenger; D004247:DNA; D003513:Cycloheximide