Experimentally induced inflammation of the guinea pig palatal mucosa by injection of a microcrystalline suspension of monosodium urate.
The injection of microcrystals has been frequently used to induce inflammatory reactions in various sites (i.e., joints, skin, pleural cavity). The aim of this study was to create a reproducible experimental animal model of buccal inflammatory reaction. The monosodium urate (MSU) crystals were obtained following the technique described by Denko and Whitehouse (1976). After injection in the palatal mucosa, biopsies were sampled and analyzed using the stereologic technique described by Weibel et al. (1966). This analysis shows 1) a decrease of the collagen volume fraction in the hours after the injection; 2) the presence of vasculitis and edema during the 12 hours after the injection; and 3) an increase in the volume fractions of the inflammatory cells, especially monocytes and polymorphonuclear leukocytes, during the same period.
D000818:Animals; D003094:Collagen / Q000378:metabolism; D004487:Edema / Q000139:chemically induced / Q000473:pathology; D005347:Fibroblasts / Q000473:pathology; D006168:Guinea Pigs; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D009000:Monocytes / Q000473:pathology; D009061:Mouth Mucosa / Q000098:blood supply / Q000187:drug effects* / Q000473:pathology; D009504:Neutrophils / Q000473:pathology; D010159:Palate / Q000473:pathology; D010950:Plasma Cells / Q000473:pathology; D013280:Stomatitis / Q000139:chemically induced* / Q000473:pathology; D013997:Time Factors; D014527:Uric Acid
D014527:Uric Acid; D003094:Collagen