title: A method for preparation of dry thrombin for topical application.
abstract: Thrombin for topical hemostasis can be prepared from bovine or human blood plasma. The prothrombin is isolated by means of adsorption on DEAE-Sephadex A-50 and consecutively activated by CaCl2 and thromboplastin. Thrombin is precipitated and purified by acetone. The specific activity of the thrombin preparation is 122 + 23 IU/mg protein while the yield is 36,360 +/- 6623 IU/liter plasma.
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D006801:Humans; D013917:Thrombin / Q000008:administration & dosage / Q000302:isolation & purification*
chemical_list: D013917:Thrombin