Three monoclonal antibodies against measles virus F protein cross-react with cellular stress proteins.
In a group of 11 monoclonal antibodies specifically reacting with the measles virus fusion protein, three antibodies also immunoprecipitated other proteins, in particular a 79,000-molecular-weight protein from virus-infected cells. The cross-reacting 79,000-molecular-weight protein was shown to be a virus-induced host stress protein. This protein could be induced by (i) different paramyxoviruses, (ii) heat shock of uninfected HeLa cells, and (iii) 2-deoxyglucose, tunicamycin, or L-canavanine treatment of different mammalian cell lines. Immunofluorescence of stressed HeLa cells localized the cross-reacting host protein(s) mainly in the cytoplasm. The significance of these results in relation to autoimmunity is discussed.
D000911:Antibodies, Monoclonal / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology; D002467:Cell Nucleus / Q000378:metabolism; D003429:Cross Reactions; D003593:Cytoplasm / Q000378:metabolism; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008459:Measles virus / Q000276:immunology*; D008970:Molecular Weight; D014759:Viral Envelope Proteins / Q000276:immunology*; D014760:Viral Fusion Proteins
D000911:Antibodies, Monoclonal; D006360:Heat-Shock Proteins; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins