DNA replication and repair in Tilapia cells. I. The effect of ultraviolet radiation.
The effect of ultraviolet radiation on a cell line established from the warm water fish Tilapia has been assessed by measuring the rate of DNA synthesis, excision repair, post-replication repair and cell survival. The cells tolerate ultraviolet radiation better than mammalian cells with respect to DNA synthesis, post-replication repair and cell survival. They are also efficient in excision repair, which in other fish cell lines has been found to be at a low level or absent. Their response to the inhibitors hydroxyurea and 1-beta-D-arabinofuranosylcytosine is less sensitive than that of other cell lines, yet the cells seem to have very small pools of DNA precursor.
D000818:Animals; D002455:Cell Division / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D006224:Cricetinae; D003561:Cytarabine / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair / Q000528:radiation effects*; D004261:DNA Replication / Q000528:radiation effects*; D005260:Female; D005399:Fishes; D006918:Hydroxyurea / Q000494:pharmacology; D007668:Kidney / Q000166:cytology; D010053:Ovary / Q000166:cytology; D014466:Ultraviolet Rays
D003561:Cytarabine; D004247:DNA; D006918:Hydroxyurea