Regulation of glutathione S-transferase mRNAs by phenobarbital and 3-methylcholanthrene: analysis using cDNA probes.
Using antibody directed against glutathione S-transferase B (YaYc heterodimer) and glutathione S-transferases A and C (Yb homodimers), we have purified the corresponding mRNAs by polysome immunoprecipitation. These mRNAs have been utilized to synthesize high specific activity cDNA probes which have been used to screen a cDNA library constructed from RNA fractions enriched in the various transferase mRNAs. Two clones which have been identified, pGTB38 and pGTA/C36, are complementary to the Ya/Yc mRNAs and Yb mRNAs, respectively. These clones have been utilized to determine that the Ya/Yc mRNAs and Yb mRNA(s) are elevated coordinately in response to xenobiotic treatment; however, their maximal level of induction is markedly different. Based upon the size of the mRNA specific for the Ya/Yc and Yb subunits, as well as the lack of homology between pGTB38 and pGTA/C36, we conclude that transferase B and transferases A and C are encoded for by distinct genes.
D000818:Animals; D004247:DNA; D004790:Enzyme Induction / Q000187:drug effects; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000235:genetics; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008748:Methylcholanthrene / Q000494:pharmacology*; D010634:Phenobarbital / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011487:Protein Conformation; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats
D012333:RNA, Messenger; D008748:Methylcholanthrene; D004247:DNA; D005982:Glutathione Transferase; D010634:Phenobarbital