Medium-chain fatty acid synthesis by goat mammary-gland fatty acid synthetase. The effect of limited proteolysis.
Fatty acid synthetase from goat mammary gland was subjected to limited proteolysis by trypsin and elastase. Both proteolytic enzymes selectively cleaved the chain-terminating thioester hydrolase component from the enzyme complex, leaving all other partial activities intact in the core peptides. Trypsin, but not elastase, caused extensive degradation of the released thioester hydrolase. The released thioester hydrolase could be purified to homogeneity by gel filtration. The molecular weight was estimated as 29 000 and the enzyme showed only significant hydrolytic activity toward long-chain acyl-CoA esters. The core peptides retained the ability to synthesize medium-chain acyl-CoA esters in the presence of 2,6-di-O-methyl-alpha-cyclodextrin. The results conclusively show that the terminating thioester hydrolase of goat mammary-gland fatty acid synthetase is not involved in termination of medium-chain-length fatty acid synthesis by this enzyme.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D064429:Fatty Acid Synthases / Q000037:antagonists & inhibitors / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D005227:Fatty Acids / Q000096:biosynthesis*; D005260:Female; D006041:Goats; D008321:Mammary Glands, Animal / Q000201:enzymology*; D008970:Molecular Weight; D010196:Pancreatic Elastase / Q000494:pharmacology*; D013379:Substrate Specificity; D013869:Thiolester Hydrolases / Q000276:immunology / Q000302:isolation & purification; D014357:Trypsin / Q000494:pharmacology*
D005227:Fatty Acids; D064429:Fatty Acid Synthases; D013869:Thiolester Hydrolases; C022738:thioesterase II; D010196:Pancreatic Elastase; D014357:Trypsin