Synthesis and characterisation of an iodinated bile-salt derivative for photoaffinity labelling.
The synthesis and characterisation of a novel iodinated bile salt derivative, 125I-labelled 3 beta-azidocholylhistamine, is described. The derivative is handled by rat liver in a similar manner to taurocholate and binding to bovine serum albumin, a well-characterised bile acid-binding protein, is demonstrated. The suitability of the derivative for photoaffinity labelling is assessed.
D000345:Affinity Labels / Q000138:chemical synthesis*; D000818:Animals; D001386:Azides / Q000138:chemical synthesis* / Q000378:metabolism; D002855:Chromatography, Thin Layer; D007457:Iodine Radioisotopes; D008099:Liver / Q000378:metabolism*; D010782:Photolysis; D051381:Rats; D011919:Rats, Inbred Strains; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014316:Tritium
D000345:Affinity Labels; D001386:Azides; D007457:Iodine Radioisotopes; D014316:Tritium; D012710:Serum Albumin, Bovine; C042780:3 beta-azidocholyl-2-iodohistamine