Characterization of three highly purified influenza virus strains by electron microscopy.
Mass determinations on highly purified influenza virus preparations were performed using the technique of scanning transmission electron microscopy. The masses of the three strains, X49, B/Singapore/222/79 and B/Hong Kong/8/73 were determined. The average value was 174 X 10(6) daltons with only small differences between the three strains. The mass of virus particles after removal of the protruding spike proteins, haemagglutinin and neuraminidase by bromelain treatment was determined to be 86 X 10(6) daltons. From the mass difference and the known molecular weight of the spike proteins the number of spikes was estimated to lie in the range 400 to 500.
D006389:Hemagglutinins, Viral / Q000032:analysis; D008854:Microscopy, Electron; D008970:Molecular Weight; D009439:Neuraminidase / Q000032:analysis; D009975:Orthomyxoviridae / Q000032:analysis / Q000302:isolation & purification / Q000648:ultrastructure*; D014759:Viral Envelope Proteins / Q000032:analysis*; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000032:analysis
D006389:Hemagglutinins, Viral; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D009439:Neuraminidase