Egg yolk-free Baird-Parker medium for the accelerated enumeration of foodborne Staphylococcus aureus.
A simplified procedure is described for the accelerated enumeration of foodborne Staphylococcus aureus. This involves the replacement of egg yolk in the Baird-Parker medium with Tween 80 and MgCl2. These compounds, along with pyruvate, allow the recovery of stressed cells of S. aureus on a medium which contains potassium tellurite, LiCl, and glycine as selective agents. Black colonies are identified as S. aureus by the simplified thermonuclease test.
D003470:Culture Media; D004530:Egg Yolk; D005260:Female; D005516:Food Microbiology; D008274:Magnesium / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media; D011136:Polysorbates; D008274:Magnesium