Angiotensin is not required for hypoxic constriction in salt solution-perfused rat lungs.
It has been reported that angiotensin II is specifically required for hypoxic vasoconstriction in rat lungs perfused with physiological salt solution. However, studies with other preparations indicate that angiotensin II does not play a necessary role in the mechanism of hypoxic vasoconstriction. In an attempt to resolve this disagreement I investigated in salt solution-perfused rat lungs whether vasoactive agents other than angiotensin II would induce hypoxic vasoconstriction, and, if so, whether the effect was due to selective action on the hypoxic mechanism or to a nonspecific increase in vascular reactivity. The results showed the development of hypoxic pressor responses after addition to perfusate of plasma, angiotensin II, KCl, vanadate, 4-aminopyridine, or norepinephrine plus propranolol. In contrast, addition of saline (control), ouabain, or tetraethylammonium chloride did not induce hypoxic vasoconstriction. Saralasin inhibited the effect of angiotensin II, but not that of plasma. Induction of responsiveness to hypoxia was associated with an increase in normoxic perfusion pressure and with potentiation of pressor responses to KCl. These results suggest that angiotensin II does not play a unique, integral role in the hypoxic mechanism, but instead is only one of many substances that will induce hypoxic pressor reactivity by reversing the vascular hyporeactivity of salt solution-perfused rat lungs.
D015761:4-Aminopyridine; D000631:Aminopyridines / Q000494:pharmacology; D000704:Analysis of Variance; D000804:Angiotensin II / Q000494:pharmacology / Q000502:physiology; D000818:Animals; D000860:Hypoxia / Q000503:physiopathology*; D008297:Male; D010477:Perfusion; D011189:Potassium Chloride / Q000494:pharmacology; D011652:Pulmonary Circulation; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride / Q000494:pharmacology; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology; D014661:Vasoconstriction / Q000187:drug effects
D000631:Aminopyridines; D014639:Vanadium; D000804:Angiotensin II; D014638:Vanadates; D012965:Sodium Chloride; D011189:Potassium Chloride; D015761:4-Aminopyridine