Function of carbohydrate moiety in acid phosphatase of Rhodotorula glutinis.
The properties of native and partially deglycosylated forms of acid phosphatase from Rhodotorula glutinis were compared. The removal of carbohydrate moiety resulted in higher thermostability and resistance to proteolysis whereas specific activity, pH optimum and Km value with p-nitrophenyl phosphate remained unchanged. The role of carbohydrate moiety in stabilization of the enzyme structure and protection against proteolysis is suggested.
D000135:Acid Phosphatase / Q000032:analysis* / Q000378:metabolism; D002241:Carbohydrates / Q000032:analysis*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D003904:Mitosporic Fungi / Q000201:enzymology*; D009596:Nitrophenols / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D012248:Rhodotorula / Q000201:enzymology*
D002241:Carbohydrates; D009596:Nitrophenols; D009943:Organophosphorus Compounds; C008644:nitrophenylphosphate; D000135:Acid Phosphatase