Isolation and amino-acid composition of the adipokinetic hormone of Manduca sexta.
An adipokinetic hormone was purified from corpora cardiaca of au adult Manduca sexta (tobacco hornworm)by reversed-phased high performance liquid chromatography. The following amino-acid composition was determined: 2 Thr, 2 Ser, Glx, Gly, Leu, Phe, Trp. The adipokinetic activity was tested in adult Manduca. In a bioassay in which activation of fat body glycogen phosphorylase was measured in ligated larvae, the hormone elicited a maximal response at the same concentration as it did in the bioassay for hyperlipaemic activity.
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D001681:Biological Assay; D007301:Insect Hormones / Q000302:isolation & purification*; D007915:Lepidoptera; D009842:Oligopeptides; D006005:Phosphorylases / Q000302:isolation & purification; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives
D000596:Amino Acids; D007301:Insect Hormones; D009842:Oligopeptides; C044833:adipokinetic hormone; C014956:adipokinetic hormone (locust); D006005:Phosphorylases; D011761:Pyrrolidonecarboxylic Acid