Implication of a peroxisomal enzyme in the catabolism of glutaryl-CoA.
A linear rate of H2O2 production is found when glutaryl-CoA is incubated with liver homogenates. We term this enzyme activity glutaryl-CoA oxidase. Its main characteristics are described and compared with those of glutaryl-CoA dehydrogenase (EC 1.3.99.7) and palmitoyl-CoA oxidase (EC 1.1.3.-). The latter enzyme catalyses the first step of peroxisomal beta-oxidation. Glutaryl-CoA oxidase shares several properties with palmitoyl-CoA oxidase. The activities of both enzymes in mouse liver are increased by feeding the animals with a clofibrate-containing diet. Subcellular fractionation of the liver homogenates on a linear sucrose gradient indicates that glutaryl-CoA oxidase is a peroxisomal enzyme.
D000214:Acyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D002500:Centrifugation, Isopycnic; D002994:Clofibrate / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D050770:Glutaryl-CoA Dehydrogenase; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D008830:Microbodies / Q000187:drug effects / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D051381:Rats; D013431:Sulfates / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology; D019287:Zinc Sulfate
D000214:Acyl Coenzyme A; D013431:Sulfates; C015901:glutaryl-coenzyme A; D019287:Zinc Sulfate; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042428:glutaryl coenzyme A oxidase; C028427:palmitoyl CoA oxidase; D050770:Glutaryl-CoA Dehydrogenase; D002994:Clofibrate; D015032:Zinc