title: Phospholipids of Golgi subfractions determined with an enzymatic method.
abstract: Golgi apparatus subfractions are still under consideration both for morphological, biochemical and functional characterization. In this note, we determined glycerophospholipids and Golgi subfractions from rat liver by a method that requires the complete enzymatic hydrolysis of phospholipids with phospholipase C from CL perfringens. The main advantages of this procedure are that small amounts of protein membranes are required, the precision and the rapidity.
mesh_terms: D000818:Animals; D005260:Female; D006056:Golgi Apparatus / Q000032:analysis*; D008099:Liver / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000032:analysis; D010738:Type C Phospholipases
chemical_list: D010743:Phospholipids; D010738:Type C Phospholipases