Binding characteristics of a fluorescent analogue of diphosphoglyceric acid to human, amphibian and fish hemoglobins.
An attempt was made to establish the binding of N-(2,4-diphosphobenzyl)-1-amino-5-naphthalenesulfonic acid, DIPANS, as an estimator of conformation in the carbonmonoxy (CO)-hemoglobins (Hbs) of several vertebrates. DIPANS failed to bind menhaden I, trout I or tuna Hbs which are ligand insensitive. Below a pH of 7.0, DIPANS bound menhaden II, Bufo, Xenopus, and human Hbs with a binding stoichiometry greater than one. The charge of the DIPANS molecule does not control its binding to these Hbs. The binding to human CO-Hb can not be due to Hb conformation. For Xenopus Hbs and menhaden II, conformation predominates DIPANS binding. The binding to CO-Hb of DIPANS, can not be unambiguously attributed to the Hb's quaternary conformation.
D000818:Animals; D002025:Bufonidae; D002263:Carboxyhemoglobin / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009282:Naphthalenesulfonates / Q000097:blood*; D011485:Protein Binding; D011487:Protein Conformation; D013045:Species Specificity; D014337:Trout; D014413:Tuna; D014981:Xenopus
D006454:Hemoglobins; D009282:Naphthalenesulfonates; C043856:N-(2,4-diphosphobenzyl)-1-amino-5-naphthalenesulfonic acid; D002263:Carboxyhemoglobin