[A new affinity sorbent for the purification of lectins and its use in the purification of wheat germ agglutinin].
A method is developed for obtaining gel from eggwhite and its application as a sorbent for purification of lectins. Eggwhite was treated by 1% glutaraldehyde at pH 5, for 5-6 hours at room temperature, then it was minced and washed by water. The residual aldehyde groups were blocked by glycine treatment. The sorbent obtained possessed high affinity for lectins specific to N-acetylglucosamine and complex oligosaccharides. The galactose- and mannose-specific lectins were adsorbed to a less extent. The purification of the wheat germ agglutinin using the eggwhite gel is described.
D000327:Adsorption; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D037102:Lectins / Q000302:isolation & purification*; D010047:Ovalbumin; D014909:Wheat Germ Agglutinins
D037102:Lectins; D014909:Wheat Germ Agglutinins; D010047:Ovalbumin
Novyĭ affinnyĭ sorbent dlia ochistki lektinov i ego primenenie dlia ochistki aggliutinina iz zarodysheĭ pshenitsy.