Influenza-C-virion-associated RNA-dependent RNA-polymerase activity.
The addition of detergent-solubilized influenza C virus to a reaction mixture containing ATP, CTP, GTP, [3H]UTP, and Mg2+ leads to the synthesis of an acid-insoluble, radioactive product, which is ribonuclease-sensitive. The dinucleoside monophosphate ApG clearly enhances the reaction rate, a fact which indicates that influenza C viruses follow the same strategy of transcription as influenza A and B viruses, the other members of the orthomyxovirus family.
D000249:Adenosine Monophosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002642:Chick Embryo; D015226:Dinucleoside Phosphates; D006151:Guanosine / Q000031:analogs & derivatives / Q000494:pharmacology; D007700:Kinetics; D008345:Manganese / Q000494:pharmacology; D009975:Orthomyxoviridae / Q000201:enzymology* / Q000235:genetics; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism*; D012265:Ribonucleotides / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D014771:Virion / Q000201:enzymology*
D015226:Dinucleoside Phosphates; D012265:Ribonucleotides; D006151:Guanosine; C031924:adenylyl-(3'-5')-guanosine; D000249:Adenosine Monophosphate; D008345:Manganese; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase; D002118:Calcium