title: Isolation and primary structure of human PHI (peptide HI).
abstract: The isolation of the human form of PHI (peptide HI) is described. The peptide was purified from human colonic extracts by using a chemical method for the detection of its C-terminal amidated structure. Human PHI consists of 27 amino acid residues and the complete amino acid sequence is: His-Ala-Asp-Gly-Val-Phe-Thr-Ser-Asp-Phe-Ser-Lys-Leu-Leu-Gly-Gln-Leu-Ser- Ala-Lys-Lys-Tyr-Leu-Glu-Ser-Leu-Met-NH2. The differences between the structures of porcine and human PHI are at position 12 (Arg/Lys replacement) and at position 27 (Ile/Met).
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000032:analysis*; D003110:Colonic Neoplasms / Q000032:analysis; D005590:Fractional Precipitation; D006801:Humans; D010451:Peptide PHI; D010455:Peptides / Q000302:isolation & purification*; D013552:Swine
chemical_list: D010451:Peptide PHI; D010455:Peptides