In vitro and in vivo evidence that the C-terminus of preproenkephalin-A circulates as an 8500-dalton molecule.
One of the major immunoreactive components of the enkephalin precursor BAM-22P in extracts of adrenal medulla is a molecule of approximately 8500 Da. A similar component is detected, after gel-permeation chromatography, in extracts of bovine plasma and in culture medium harvested from acetylcholine-stimulated bovine adrenomedullary cells in culture. Simultaneous detection of the C-terminal epitome of preproenkephalin-A: Met-enkephalin-Arg6-Phe7-OH in this secreted high-molecular-weight zone suggest that the approximately 8500-Da form of BAM-22P is the C-terminal of preproenkephalin A.
D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004745:Enkephalins / Q000097:blood* / Q000378:metabolism; D008970:Molecular Weight; D010446:Peptide Fragments / Q000097:blood / Q000378:metabolism; D011498:Protein Precursors / Q000097:blood* / Q000378:metabolism; D011863:Radioimmunoassay
D004745:Enkephalins; D010446:Peptide Fragments; D011498:Protein Precursors; C033660:preproenkephalin