title: Ion-exchange derivatives of Spheron. V. Sulphate and sulpho derivatives.
abstract: Using various reactions, strongly acidic derivatives of Spheron of the sulphate and sulpho types were prepared with nominal capacities between 0.37 and 2.0 mequiv./g. They were characterized by their capacities for small ions, by their static and dynamic capacities for proteins, by elemental analysis, by their working volumes and specific internal surface areas. The differences between the nominal capacities calculated theoretically from the sulphur contents and the measured values are discussed, as are the relationships between the nominal capacities for small ions and the static and dynamic capacities for proteins. The chromatographic properties of the cation exchangers are illustrated by the separation of an artificial mixture of proteins (serum albumin, chymotrypsinogen and lysozyme) at various pH values, and of a natural mixture of egg-white proteins. The separation of active enzymes is also reported. Sulphobutyl-Spheron was tested also as a catalyst for the esterification of alcohols.
mesh_terms: D000818:Animals; D002384:Catalysis; D002412:Cations / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D004798:Enzymes / Q000302:isolation & purification; D020100:Hydrogels; D006863:Hydrogen-Ion Concentration; D010047:Ovalbumin / Q000302:isolation & purification; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives / Q000032:analysis; D011109:Polymethacrylic Acids / Q000032:analysis*; D011506:Proteins / Q000302:isolation & purification; D012709:Serum Albumin / Q000302:isolation & purification; D013431:Sulfates / Q000032:analysis*; D013696:Temperature
chemical_list: D002412:Cations; D004798:Enzymes; D020100:Hydrogels; D011109:Polymethacrylic Acids; D011506:Proteins; D012709:Serum Albumin; D013431:Sulfates; C000257:polyhydroxyethyl methacrylate hydrogels; D011102:Polyhydroxyethyl Methacrylate; C012553:spherons; D010047:Ovalbumin