title: [Biotransformation of monolinuron (N-(4-chlorophenyl)-N'-methyl-N'methoxyurea) in isolated perfused chicken liver].
abstract: The biotransformation of radioactively labelled monolinuron (N-(4-chloro[U-14C]phenyl)-N'-methyl-N'-methoxyurea) was studied in the isolated perfused liver of the chicken. After a 4-hr perfusion, 83.1% of the added radioactivity was recovered, 56.6% in the perfusion medium and 26.5% in the liver and bile. The fraction of radioactivity extractable from the perfusion medium into ethyl acetate amounted to 47.8% of the added dose. In addition to monolinuron, five breakdown products were identified in this extract, namely N-(4-chlorophenyl)-N'-hydroxymethyl-N'-methoxyurea, N-(4-chlorophenyl)-N'-methoxyurea, N-(4-chlorophenyl)-N'-methylurea, 4-chlorophenylurea and 4-chloroacetanilide. Of particular interest was the absence of arylhydroxylated monolinuron derivatives, since in monolinuron-metabolism studies in the laying hen 2-hydroxy-4-chlorophenylurea and 3-hydroxy-4-chlorophenylurea were both detected. This differing metabolism corresponds to earlier findings in the rat, in which arylhydroxylated breakdown products were detected only in in vivo studies and not in rat-liver perfusion. Possible reasons for the differing metabolism of monolinuron in vivo and in vitro are discussed.
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism; D001711:Biotransformation; D002250:Carbon Radioisotopes; D002645:Chickens; D002855:Chromatography, Thin Layer; D005260:Female; D006540:Herbicides / Q000378:metabolism*; D066298:In Vitro Techniques; D008044:Linuron / Q000031:analogs & derivatives / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D010477:Perfusion
chemical_list: D002250:Carbon Radioisotopes; D006540:Herbicides; D008044:Linuron; C030651:monolinuron
vernacular_title: Biotransformation von Monolinuron (N-(4-Chlorphenyl)-N'-methyl-N'-methoxyharnstoff) in der isoliert perfundierten Hühnerleber.