[Isolation and characterization of individual tRNA Leu 1,2,and 4 from the bovine udder].
tRNA-Leu 1, tRNA-Leu 2 and tRNA-Leu 4 were isolated from a lactating cow mammary gland by combination of several chromatographic methods. Chromatography on a Sepharose 4B column with a reverse salt gradient was used as the first step. Individual tRNAsLeu were further purified by RPC-5 column chromatography at pH 4.5 and 7.5. For isolation of tRNA-Leu 2 a RPC-5 column was additionally used at pH 3.3 in the presence of 7 M urea. Using micro-column chromatography of Ti-RNAases digests, it was demonstrated that tRNA-Leu 1 and tRNA-Leu 2+ are similar in their primary structure and differ essentially from tRNA-Leu 4.
D000818:Animals; D002417:Cattle; D002845:Chromatography / Q000379:methods; D002847:Chromatography, Agarose; D005260:Female; D007774:Lactation; D008321:Mammary Glands, Animal / Q000032:analysis*; D011247:Pregnancy; D012346:RNA, Transfer, Amino Acyl / Q000302:isolation & purification*
D012346:RNA, Transfer, Amino Acyl
Vydelenie i kharakteristika individual'nykh tRNK Leu1,2,4 iz molochnoĭ zhelezy korov.