title: Flow cytometric analysis of lectin-binding to canine blood lymphocytes.
abstract: The cell surface glycoconjugates of blood lymphocytes from 19 dogs with and without neoplastic disease were quantitated using flow cytofluorometric analysis of the binding characteristics of 3 lectins, namely, wheat germ agglutinin, concanavalin-A, and Lens culinaris agglutinin. The specificity of lectin binding was determined using competitive monosaccharide inhibitors. The results show enhanced binding of concanavalin-A to blood lymphocytes from dogs with lymphosarcoma relative to healthy dogs, or those with a variety of neoplastic and nonneoplastic diseases.
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000378:metabolism; D004285:Dogs; D005434:Flow Cytometry; D006023:Glycoproteins / Q000378:metabolism; D037102:Lectins; D008214:Lymphocytes / Q000378:metabolism*; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism / Q000662:veterinary; D008565:Membrane Proteins / Q000378:metabolism; D011485:Protein Binding
chemical_list: D006023:Glycoproteins; D037102:Lectins; D008565:Membrane Proteins