Synthesis of novel calcium-dependent proteins associated with mammary epithelial cell migration and differentiation.
A class of proteins from mouse mammary epithelial cells has been isolated which, like the calcium-binding protein calmodulin (CaM), binds to phenothiazine in a calcium-dependent manner. These proteins do not bind to phenothiazine through binding to CaM; we infer that they are calcium-binding proteins, and that they may be related to the similarly isolated 'calcimedins' of Moore, P D & Dedman, J, J biol chem 257 (1982) 9663 [8]. In primary cultures of mouse mammary cells on collagen gels, synthesis of certain of these proteins is associated with the spreading of cells to form monolayers; failure of cells to spread and differentiate, through omission of serum from culture medium, results in the inhibition of calcium-binding protein synthesis, with the exception of CaM and a 15 kD species. The CaM/15 kD pair are prominent during all phases of culture, and are secreted during the secretory differentiation phase of culture (floating gels). We propose that these calcium-binding proteins play a specific role in the motility of mammary epithelial cells and that they may also be involved in mammary secretory differentiation.
D000818:Animals; D002135:Calcium-Binding Proteins / Q000096:biosynthesis*; D002147:Calmodulin; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D010640:Phenothiazines
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D010640:Phenothiazines