title: The isolation and characterization of cDNA clones for human apolipoprotein CII.
abstract: Recombinant clones containing cDNA sequences coding for human apolipoprotein CII have been isolated from a human adult liver cDNA library. The clones were detected by hybridization with a mixed probe consisting of 96 different oligonucleotides 17 bases long, complementary to the mRNA coding for amino acids 66 to 71 of apolipoprotein CII. The largest cDNA insert is 439 nucleotides long and contains the complete sequence for the mature protein, the 3' untranslated sequence, and sequence coding for 17 amino acids of a signal peptide. The cDNA clone was used to detect apolipoprotein CII mRNA approximately 500 bases long in human liver and intestine RNA.
mesh_terms: D000595:Amino Acid Sequence; D053304:Apolipoprotein C-II; D001053:Apolipoproteins / Q000235:genetics*; D001056:Apolipoproteins C; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D053304:Apolipoprotein C-II; D001053:Apolipoproteins; D001056:Apolipoproteins C; D012333:RNA, Messenger; D004247:DNA