Newcastle disease virus: the effect of monoclonal antibody in the overlay on virus penetration and the immunoselection of variants.
Monoclonal antibody to the haemagglutinin-neuraminidase or the fusion glycoprotein of Newcastle disease virus blocked virus penetration which otherwise occurred within 90 s of the temperature being increased from 4 degrees C to 37 degrees C. These antibodies regularly immunoselected variant plaques only when incorporated into the agarose overlay. Variant viruses then formed plaques whereas the growth of non-neutralized normal virus was suppressed.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D002417:Cattle; D002460:Cell Line; D006389:Hemagglutinins, Viral / Q000276:immunology; D009154:Mutation; D009439:Neuraminidase / Q000276:immunology; D009500:Neutralization Tests; D009522:Newcastle disease virus / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D012641:Selection, Genetic; D013696:Temperature; D014759:Viral Envelope Proteins / Q000276:immunology; D014760:Viral Fusion Proteins; D010948:Viral Plaque Assay
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D009439:Neuraminidase