Rat lung lavage surfactant enhances bacterial phagocytosis and intracellular killing by alveolar macrophages.
We have tested the effect of a preparation of rat lung lavage surfactant (SAM) on phagocytosis and intracellular killing of Staphylococcus aureus by rat alveolar macrophages (AMs). The SAM was isolated and purified by density gradient centrifugation. It was highly enriched in disaturated phosphatidylcholine, and invariably lowered the surface tension of a clean saline solution to less than 10 dynes/cm at 37 degrees C. We used a radiometric assay to measure phagocytosis as uptake by AMs of 14C phenylalanine labelled staphylococci, and intracellular killing as incorporation of 3H thymidine by viable staphylococci from the lysed AMs. When staphylococci were incubated with 100 to 300 micrograms of SAM, both phagocytosis and intracellular killing efficiency were enhanced. The mean numbers of intracellular staphylococci/AM were 24.3 +/- 3.8 and 18.3 +/- 3.0 in the SAM and control groups, respectively (p less than 0.001). The mean intracellular killing efficiency was 59.6 +/- 6.76% and 39.7 +/- 7.17% in the SAM and control groups, respectively (p less than 0.001). Previous studies have shown that unpurified rat lung lavage fluid enhances intracellular killing of staphylococci by AMs. Our results suggest that SAM may be the active principle in lung lavage fluid that enhances intracellular killing. In addition, in our test system, SAM enhanced staphylococcal phagocytosis by AMs.
D000818:Animals; D008264:Macrophages / Q000276:immunology*; D008297:Male; D010587:Phagocytosis / Q000187:drug effects*; D011650:Pulmonary Alveoli / Q000166:cytology; D011663:Pulmonary Surfactants / Q000032:analysis / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013211:Staphylococcus aureus / Q000276:immunology*; D007507:Therapeutic Irrigation
D011663:Pulmonary Surfactants