Studies on simian hemorrhagic fever virus nucleocapsids.
Purified Simian Hemorrhagic Fever (SHF) virions are shown to have a sedimentation coefficient of 214S when compared with previously determined figures for Sindbis virus cosedimented in neutral sucrose gradients. The nucleic acid content in SHF virions was about 8 per cent of the total virionic mass. Nucleocapsids obtained by treating SHF virions with nonionic detergent and analyzed by ultracentrifugation in neutral sucrose gradients gave RNAse sensitive- but DNAse resistent particles with a reproducible sedimentation coefficient of 174S and a buoyant density of 1.33 g/ml in cesium chloride gradients.
D000818:Animals; D002213:Capsid / Q000032:analysis*; D003902:Detergents; D006482:Hemorrhagic Fevers, Viral / Q000382:microbiology / Q000662:veterinary*; D008992:Monkey Diseases / Q000382:microbiology*; D012367:RNA, Viral / Q000032:analysis; D012260:Ribonucleases; D014035:Togaviridae / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis
D003902:Detergents; D012367:RNA, Viral; D014764:Viral Proteins; D012260:Ribonucleases