title: Characterization of the effects of cultured vascular cells on the activation of blood coagulation.
abstract: The coagulant properties of intact bovine vascular cells (aortic endothelial and smooth muscle cells) and human vascular cells (cutaneous and foreskin microvascular cells, umbilical venous endothelium) grown in vitro were studied. Compared to nonvascular cells (fibroblasts, corneal endothelial cells, fetal lung or intestinal mucosal cells), vascular cells had little procoagulant activity. Radioimmunologic measurement of thrombin in recalcified plasma demonstrated markedly lower concentrations of thrombin in the presence of vascular endothelial and smooth muscle cells compared to corneal endothelial and fetal lung cells. The low thrombin concentrations were not a consequence of thrombin binding to the vascular cells nor were they due to accelerated thrombin inactivation by antithrombin-III or alpha 2-macroglobulin. Neither vascular cells nor the nonvascular cells promoted contact activation of plasma as measured by a sensitive specific assay for kallikrein. Studies with intact cell monolayers and purified factors VIIa and X indicated that while nonvascular cells express tissue factor activity, vascular cells do not exhibit this property. These data suggest that the nonthrombogenic nature of intact vascular cells is due to their failure to initiate contact activation and to express tissue factor activity. In addition, the primary difference in coagulant potential between vascular cells and nonvascular cells is the lack of tissue factor expression by the vascular cells.
mesh_terms: D000818:Animals; D000990:Antithrombin III / Q000502:physiology; D001011:Aorta / Q000166:cytology*; D001777:Blood Coagulation; D002417:Cattle; D046508:Culture Techniques; D004727:Endothelium / Q000166:cytology; D005347:Fibroblasts; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology; D007610:Kallikreins / Q000502:physiology; D008168:Lung / Q000166:cytology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D013917:Thrombin / Q000096:biosynthesis; D013925:Thromboplastin / Q000502:physiology; D014471:Umbilical Veins / Q000166:cytology*
chemical_list: D000990:Antithrombin III; D013925:Thromboplastin; D007610:Kallikreins; D013917:Thrombin