Imaging of biological structures with the scanning transmission electron microscope.
The scanning transmission electron microscope (STEM) is discussed in view of biological applications. Theoretical considerations are given, but the emphasis is directed to practical examples from a range of biological projects. The STEM is most efficiently used in elastic and inelastic dark-field modes providing information on the scattering power of the irradiated sample. Thus, the STEM is an ideal tool for quantitative measurements such as mass-mapping or element-mapping at high resolution. Limitations of such methods due to multiple scattering and quantum noise are briefly reviewed.
D000818:Animals; D001419:Bacteria / Q000648:ultrastructure; D001792:Blood Platelets / Q000648:ultrastructure; D002843:Chromatin / Q000648:ultrastructure; D008099:Liver / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation / Q000379:methods*; D051381:Rats; D012542:Scattering, Radiation; D013604:T-Phages / Q000648:ultrastructure
D002843:Chromatin