Identification of Thormählen-positive compound "B" in urine of patients with malignant melanoma.
The identification of the entire structure of the Thormählen-positive compound B from melanotic urine is described. The compound was separated from other Thormählen-positive compounds using DEAE-cellulose column chromatography. On the basis of differential enzymic hydrolysis followed by gas chromatography-mass spectrometry analysis and comparison with synthetically prepared compounds it is possible to conclude that the Thormählen-positive compound B is a mixture of O-sulphate 5-hydroxy-6-methoxyindole and 6-hydroxy-5-methoxyindole with predominance of the latter.
D002848:Chromatography, DEAE-Cellulose; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007211:Indoles / Q000302:isolation & purification* / Q000652:urine; D008543:Melanins / Q000302:isolation & purification* / Q000652:urine; D008545:Melanoma / Q000652:urine*; D012878:Skin Neoplasms / Q000652:urine*
D007211:Indoles; D008543:Melanins; C025049:Thormaehlen positive melanogens; C042567:Thormaehlen positive compound B