Separation of choline- and ethanolamine-labeled metabolites by ion-exchange high-pressure liquid chromatography.
Two methods utilizing ion-exchange high-performance liquid chromatography have been developed for the separation of choline- and ethanolamine-containing metabolic precursors for phosphatidylcholine and phosphatidylethanolamine. Both methods employ an analytical anion-exchange column supplemented with a cation-exchange column; the latter is required only to separate betaine from choline. Complete separation of choline- and ethanolamine-labeled metabolites from extracts of Chinese hamster ovary cells can be achieved in 20 min.
D000818:Animals; D002460:Cell Line; D002794:Choline / Q000031:analogs & derivatives* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006224:Cricetinae; D003412:Cricetulus; D004983:Ethanolamines / Q000302:isolation & purification*; D005260:Female; D010053:Ovary; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism
D004983:Ethanolamines; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D002794:Choline