Phospholipids as acyl donors to membrane proteins of Mycoplasma capricolum.
Mycoplasma capricolum, a procaryotic sterol and fatty acid auxotroph, contains a large number of membrane proteins covalently modified by both saturated and unsaturated fatty acids (Dahl, C.E., Dahl, J.S., and Bloch, K. (1983) J. Biol. Chem. 258, 11814-11818). Pulse-chase experiments show that the radioactivity in the fatty acid moieties of the acyl proteins increases rather than decreases during a 4.5-h incubation period suggesting that a large intracellular pool of metabolites such as phospholipid serves as the donor for protein acylation. We find that cells incubated for 4 h in a growth medium containing [3H]palmitate-labeled phosphatidylglycerol or 2-[3H]palmitoyl dipalmitoylphosphatidylcholine show the same labeling pattern as cells incubated for 4 h in a complete growth medium with [3H]palmitate. Exogenously added phospholipids are not hydrolyzed to free fatty acid during the labeling period. Acylation of proteins is inhibited in cells treated with chloramphenicol showing that there is no pool of proacyl protein in the cell. Labeling of membrane proteins also occurs with [3H]glycerol. Glycerol is incorporated primarily into the same proteins as oleate suggesting that acylation by unsaturated fatty acid may involve a protein bound diglyceride moiety. Palmitate, on the other hand, appears to bind to other sites along the polypeptide chain in addition to the diglyceride moiety.
D000215:Acylation; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002701:Chloramphenicol / Q000494:pharmacology; D005990:Glycerol / Q000378:metabolism; D007700:Kinetics; D008081:Liposomes; D008565:Membrane Proteins / Q000096:biosynthesis*; D009174:Mycoplasma / Q000187:drug effects / Q000378:metabolism*; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D011663:Pulmonary Surfactants / Q000378:metabolism; D014316:Tritium
D008081:Liposomes; D008565:Membrane Proteins; D009829:Oleic Acids; D010169:Palmitic Acids; D010743:Phospholipids; D011663:Pulmonary Surfactants; D014316:Tritium; D019301:Oleic Acid; D019308:Palmitic Acid; D002701:Chloramphenicol; D005990:Glycerol