S-(1,2-dicarboxyethyl)glutathione and S-(1,2-dicarboxyethyl)L-cysteine in lens.
S-(1,2-Dicarboxyethyl)glutathione and S-(1,2-dicarboxyethyl)L-cysteine were determined by high performance liquid chromatography after reaction with 2,4-dinitrofluorobenzene. By this method the former could be determined in the range 4.05 mumol/l-815 mumol/l, and the latter in the range 1.45 mumol/l-1.45 mmol/l. The recovery from cattle lens homogenate was 90.0 +/- 3.2% for S-(1,2-dicarboxyethyl)glutathione and 95.3 +/- 3.1% for S-(1,2-dicarboxyethyl)L-cysteine. Using this method S-(1,2-dicarboxyethyl)-glutathione and S-(1,2-dicarboxyethyl)L-cysteine were determined in lenses of several vertebrates and in rat lens during cataract formation by galactose.
D000818:Animals; D002386:Cataract / Q000139:chemically induced / Q000378:metabolism*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003545:Cysteine / Q000031:analogs & derivatives* / Q000032:analysis; D004139:Dinitrofluorobenzene; D004285:Dogs; D005690:Galactose; D005978:Glutathione / Q000031:analogs & derivatives* / Q000032:analysis; D006168:Guinea Pigs; D007908:Lens, Crystalline / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D014413:Tuna
C041581:S-(1,2-dicarboxyethyl)glutathione; C017212:S-(1,2-dicarboxyethyl)cysteine; D004139:Dinitrofluorobenzene; D005978:Glutathione; D003545:Cysteine; D005690:Galactose