Orthovanadate induces loss of muscarinic cholinergic binding sites.
In homogenates of corpus striatum from rats, incubated in the presence of adenosine triphosphate (2 mM) (45 min, 37 degrees C), a loss (approx. 50%) of muscarinic cholinergic binding sites could be detected using a 3H-3-Quinuclidinyl benzilate (3H-3-QNB) binding assay. We found that only vanadium-contaminated ATP induced loss of muscarinic binding sites. Orthovanadate (1 mM) alone in the absence of ATP induced loss (approx. 60%) of binding sites just as "vanadium-ATP" did.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011950:Receptors, Cholinergic / Q000187:drug effects*; D011976:Receptors, Muscarinic / Q000187:drug effects*; D013696:Temperature; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology*
D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D014639:Vanadium; D014638:Vanadates; D011813:Quinuclidinyl Benzilate; D000255:Adenosine Triphosphate