Determination of delta-aminolaevulinic acid in biological fluids by gas-liquid chromatography with electron-capture detection.
A derivative of delta-aminolaevulinic acid (AmLev), 2-methyl-3-acetyl-4-(3-propionic acid pentafluorobenzyl ester)pyrrole, with favourable properties for g.l.c. with electron-capture detection, was synthesized. Less than 1 pg could be detected on the column. 6-Amino-5-oxohexanoic acid formed the analogous derivative under similar conditions and was used as the internal standard in the development of a highly sensitive and specific assay for AmLev. The method has been applied to peripheral-venous and umbilical-cord plasma and to cerebrospinal fluid of normal and porphyric subjects.
D015119:Aminocaproic Acid; D000622:Aminolevulinic Acid / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D001826:Body Fluids / Q000032:analysis*; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004583:Electrons; D006801:Humans; D007982:Levulinic Acids / Q000032:analysis*; D011164:Porphyrias / Q000134:cerebrospinal fluid; D011758:Pyrroles / Q000138:chemical synthesis; D013057:Spectrum Analysis; D005466:Fluorocarbons
D015119:Aminocaproic Acid; D000622:Aminolevulinic Acid; D007982:Levulinic Acids; D011758:Pyrroles; C041864:pentafluorobenzyl 2-methyl-3-acetyl-4-(3-propionate)pyrrole; D005466:Fluorocarbons