[Antihyperlipidemic effect of iodine egg: search for active ingredients and their iodine contents].
Active fractions that possess the hypocholesterolemic effect of iodine egg yolk lipid fraction have been investigated with male Wistar strain rats fed on a cholesterol-rich diet. Iodine egg yolk lipid was extracted by Folch's (chloroform: methanol) method and was refractionated into a neutral lipid (IEY-NL) fraction and a polar lipid (IEY-PL) fraction. The iodine content in each fraction was determined. The animals were kept on the cholesterol-rich diet for 5 days, and then they were fed the fractions in addition to the above diet for 10 more days. The hypocholesterolemic effect was seen most significantly with the IEY-NL fraction. The serum total cholesterol level in rats receiving this fraction was 70% as compared with that of the Ch group (fed on the cholesterol diet). However, the liver total cholesterol level was not affected or rather increased by the IEY-NL fraction. Another fraction (IEY-PL) showed no such effect. Iodine content in the IEY-NL fraction was quite low (0.2 ppm) compared with the other fractions. The daily dose in terms of iodine in the IEY-NL fraction was only the amount equivalent to 1/50 of the reported daily requirement for the rat. The IEY-NL fraction induced the hypocholesterolemic effect, though it contained only a trace of iodine.
D000818:Animals; D005591:Chemical Fractionation; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D004530:Egg Yolk / Q000032:analysis*; D005260:Female; D000960:Hypolipidemic Agents; D007455:Iodine / Q000032:analysis / Q000494:pharmacology*; D008055:Lipids / Q000302:isolation & purification / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000960:Hypolipidemic Agents; D008055:Lipids; D007455:Iodine; D002784:Cholesterol