Forskolin action on histamine stimulated adenylyl cyclase in parietal cells of rat and guinea pig.
Forskolin causes a concentration dependent stimulation of adenylyl cyclase in rat and guinea pig parietal cells; guinea pig enzyme has higher activity than rat enzyme, both in the absence and presence of forskolin. Forskolin has a potentiating effect on histamine and epinephrine stimulated enzyme activity in rat cells, but only on histamine stimulated activity in guinea pig cells. Cimetidine, an H2-type antihistamine, does not block the stimulatory action of forskolin. It is speculated that quantitative and qualitative differences in hormone receptors may be magnified with forskolin.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002927:Cimetidine / Q000494:pharmacology; D005576:Colforsin; D004224:Diterpenes / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D010295:Parietal Cells, Gastric / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
D004224:Diterpenes; D005576:Colforsin; D002927:Cimetidine; D006632:Histamine; D000262:Adenylyl Cyclases; D004837:Epinephrine