Muscarinic cholinergic receptors in a rat phaeochromocytoma cell line.
The high affinity binding of the muscarinic ligand 3H-N-methylscopolamine (3H-NMS) to intact cells of the rat pheochromocytoma cell line PC12 was studied. A single class of saturable binding sites was observed with a density of about 5000 sites per cell, and a pharmacological profile consistent with muscarinic receptors. The inhibition of 3H-NMS binding by the antagonist pirenzepine was of low affinity suggesting PC12 muscarinic receptors may be similar to those found on rat sympathetic nerve terminals.
D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology; D002460:Cell Line; D019832:N-Methylscopolamine; D010673:Pheochromocytoma / Q000378:metabolism*; D010890:Pirenzepine; D051381:Rats; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism*; D012602:Scopolamine Derivatives / Q000378:metabolism
D001570:Benzodiazepinones; D011976:Receptors, Muscarinic; D012602:Scopolamine Derivatives; D010890:Pirenzepine; D019832:N-Methylscopolamine