Identification of messenger RNA for human type II collagen.
Total RNA was purified from human fetal calvaria and articular cartilage. Messenger RNAs for type I and II collagens were identified by hybridization using cDNA clones for chicken pro alpha 1(I)-, pro alpha 2(I)- and pro alpha 1(II)collagen mRNAs and by analysis of cell-free translation products of these RNAs by polyacrylamide gel electrophoresis. The size of human pro alpha 1(II)collagen mRNA is approx. 5100 bases. Translatability of cartilage specific type II collagen mRNA was found to be concentration dependent: with increasing total RNA concentrations the relative translation of type II collagen mRNA was reduced with respect to type I mRNAs.
D000818:Animals; D002356:Cartilage / Q000032:analysis* / Q000196:embryology; D002474:Cell-Free System; D002642:Chick Embryo; D003094:Collagen / Q000235:genetics*; D004247:DNA; D006801:Humans; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*
D012333:RNA, Messenger; D003094:Collagen; D004247:DNA