title: Evidence for a nickel-containing carbon monoxide dehydrogenase in Methanobrevibacter arboriphilicus.
abstract: In growing cultures of Methanobrevibacter arboriphilicus (Methanobrevibacter arboriphilus), the synthesis of active carbon monoxide dehydrogenase required nickel. The 21-fold-purified enzyme from 63Ni-labeled cells of M. arboriphilicus comigrated with 63Ni during gel filtration. These results provide evidence that the carbon monoxide dehydrogenase of methanogens is a nickel protein.
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D002850:Chromatography, Gel; D019605:Euryarchaeota / Q000201:enzymology*; D009097:Multienzyme Complexes; D009532:Nickel / Q000032:analysis* / Q000494:pharmacology
chemical_list: D009097:Multienzyme Complexes; C022838:nickel chloride; D009532:Nickel; D000445:Aldehyde Oxidoreductases; C019823:carbon monoxide dehydrogenase