[Effect of removal of serum from the culture medium and sublethal roentgen irradiation on microvilli and invaginations of the cell membrane of Ehrlich ascites tumor cells in monolayer cultures].
In order to find out modifications of microvilli and invaginations, the cellular surfaces of Ehrlich ascites tumor cells in monolayer culture (basal medium of Eagle + 10% fetal calf serum) were investigated with the aid of electron-microscopic cross-sections. The tumor cells had been cultured without serum 24 hours prior to investigation or irradiated with 2 Gy. Morphometric evaluation after cell culture in a serum-free medium showed a reduced number ob microvilli and a diminution of sections of microvilli. As already described before, a reduction of cell proliferation, of the microtubule-microfilament system, and of the endocytosis activity occurs under these serum-free conditions. The number of invaginations (related to a constant membrane part) was reduced by nearly 50% after serum extraction. Similarly to serum extraction, sublethal X-ray irradiation reduced the sections of microvilli, whereas the number of microvilli increased slightly. Contrary to the effect of serum extraction, the irradiated cells showed twice as many invaginations as the non-irradiated control cells. These differences in the surface structures are interpreted as a result of modified growth stimulations (+/- serum) and radiogenic reparation processes.
D000818:Animals; D001798:Blood Proteins / Q000502:physiology; D002286:Carcinoma, Ehrlich Tumor / Q000532:radiotherapy*; D002462:Cell Membrane / Q000528:radiation effects*; D002478:Cells, Cultured; D003470:Culture Media; D003599:Cytoskeleton / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004705:Endocytosis / Q000528:radiation effects; D051379:Mice; D008854:Microscopy, Electron; D008870:Microtubules / Q000528:radiation effects; D008871:Microvilli / Q000528:radiation effects*
D001798:Blood Proteins; D003470:Culture Media
Einfluss von Serumentzug aus dem Kulturmedium und subletaler Röntgenbestrahlung auf Mikrovilli und Invaginationen der Zellmembran von Ehrlich-Aszites-Tumorzellen in Monolayerkultur.