title: Sodium cholate interactions with rabbit's pulmonary surfactant.
abstract: In order to assay the possibility that sodium cholate interacts with pulmonary surfactant, we obtained bronchoalveolar lavage fluid from lungs of adult rabbits and measured the hysteresis area of surface tension-area loops of the bronchoalveolar lavage fluid in a Wilhelmy surface tension balance, before and after the addition of sodium cholate to reach different concentrations. We observed a biphasic behavior: at a low concentration of sodium cholate (1.5 x 10(5) mol/l; n = 6) the hysteresis area increased (p less than 0.05) as compared to its control (initial) area, meanwhile at a higher concentration (5 x 10(-5) mol/l; n = 6) the hysteresis area decreased (p less than 0.025), revealing a likely interaction of sodium cholate with pulmonary surfactant. We conclude that sodium cholate is able to interact in vitro with lung surfactant.
mesh_terms: D000818:Animals; D019826:Cholic Acid; D002793:Cholic Acids / Q000378:metabolism*; D066298:In Vitro Techniques; D008168:Lung / Q000378:metabolism*; D011663:Pulmonary Surfactants / Q000378:metabolism*; D011817:Rabbits; D013500:Surface Tension; D007507:Therapeutic Irrigation
chemical_list: D002793:Cholic Acids; D011663:Pulmonary Surfactants; D019826:Cholic Acid