[Mapping of the transferrin gene in laboratory rats, mice and man by direct in situ hybridization].
Mapping of the gene coding for transferrin was carried out in metaphase chromosomes from bone marrow of laboratory mice and rats as well as from PHA-stimulated human lymphocytes using direct in situ hybridization technique. Plasmid pRTf-17 carrying the insert of rat transferrin cDNA was nick-translated with [125I]dCTP and used as a specific hybridization probe. The total number of silver grains and their distribution along differentially stained chromosomes were determined in 464 metaphase plates (114, 263 and 87 from rat, mouse and man, respectively). The data obtained enable us to assign transferrin gene to chromosome 3 in human and chromosome 9 in mouse. For the first time, the rat transferrin gene was localized on chromosome 7. The most probable sites of transferrin gene localization are 7q31-34, 9F1-3 and 3q21 in rat, mouse and human chromosomes, respectively.
D000818:Animals; D001435:Bacteriophages / Q000235:genetics; D001853:Bone Marrow / Q000648:ultrastructure; D002478:Cells, Cultured; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005796:Genes; D006720:Homozygote; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D008677:Metaphase; D051379:Mice; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D051381:Rats; D014168:Transferrin / Q000235:genetics*; D014178:Translocation, Genetic
D004269:DNA, Bacterial; D014168:Transferrin; D004247:DNA
Kartirovanie gena transferrina u laboratornykh krys, mysheĭ i cheloveka metodom priamoĭ gibridizatsii in situ.