Are there diurnal fluctuations in crypt length and crypt cell birth rate in the intestines of normal and carcinogen-treated rats?
At eight time points over a period of 24 hours, crypt length (in cells) and crypt cell birth rate were measured by the stathmokinetic method with vincristine and evaluated at two sites in the small intestine and two in the colon in Wistar Porton rats. Normal animals were compared with animals which had received 24 injections, at weekly intervals, of the intestinal carcinogen 1,2-dimethylhydrazine. In the treated animals, crypts were significantly longer at sites prone to tumour formation but not at those sites known to be resistant to the effects of the carcinogen. In neither group of animals was there any significant fluctuation in mean crypt length over a 24 hours period. Crypt cell birth rates showed a considerable fluctuation. No difference was noted between normal animals and those treated with dimethylhydrazine and statistical analysis failed to confirm the presence of any true periodic fluctuation.
D019813:1,2-Dimethylhydrazine; D000818:Animals; D001699:Biometry; D002273:Carcinogens / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002940:Circadian Rhythm; D004127:Dimethylhydrazines / Q000494:pharmacology*; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D005260:Female; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D007414:Intestinal Neoplasms / Q000473:pathology; D008763:Methylhydrazines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D002273:Carcinogens; D004127:Dimethylhydrazines; D008763:Methylhydrazines; D019813:1,2-Dimethylhydrazine