title: Cell proliferation in the dog (beagle) ovary during proestrus and early estrus.
abstract: Cell proliferation in the ovaries of 5 Beagle dogs was studied by autoradiography after pulse labelling with (3H)-thymidine during different periods of proestrus and early estrus. Indices of labelling and of necrosis were determined for different follicle types grouped according to their stages of atresia. Cell proliferation became most obvious during early proestrus in antral and in Graafian follicles, and during the periovulatory period in preantral follicles. By contrast, low labelling indices appeared in all follicle types during the middle of proestrus. All follicle types demonstrated a continuous decrease in labelling from early to late atresia with the exception of the granulosal layer of antral follicles during the periovulatory period. While many necrotic granulosal cells were found in antral follicles during the first 6 days of proestrus, there were few during the periovulatory period. Even Graafian follicles displayed necrotic granulosal cells now and then. It may be concluded that (1) intact ovarian follicles can show subtle changes indicating the beginning of atresia, and (2) during the proliferative phase of a long estrous cycle cell replication in the ovary is characterized by two bursts at the beginning and at the end of this period.
mesh_terms: D000818:Animals; D001345:Autoradiography; D002455:Cell Division; D004285:Dogs / Q000033:anatomy & histology* / Q000502:physiology; D004971:Estrus; D005260:Female; D006080:Ovarian Follicle / Q000166:cytology; D010053:Ovary / Q000166:cytology*; D011247:Pregnancy; D011359:Proestrus; D013799:Theca Cells / Q000166:cytology; D013936:Thymidine
chemical_list: D013936:Thymidine