The sequence of an atriopeptigen: a precursor of the bioactive atrial peptides.
The high molecular weight fraction ( atriopeptigen -APG) obtained by gel filtration chromatography of rat atrial extracts was fractionated by isoelectric focusing and reverse phase HPLC to obtain a pure APG. Purification of cyanogen bromide digests of the crude high molecular weight fraction resulted in the isolation of a single biologically active cyanogen bromide cleavage peptide. Sequence analyses of these peptides coupled with recent reports of sequence analyses of intermediate molecular weight atrial peptides ( Thibault , et al. (1984) FEBS Letters 167, 352-356, and Kangwa , et al., Biochem. Biophys. Res. Commun 119, 933-940) provide the complete primary structure of an 111 residue APG.
D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D006325:Heart Atria / Q000032:analysis; D008970:Molecular Weight; D009124:Muscle Proteins / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000302:isolation & purification; D011498:Protein Precursors / Q000302:isolation & purification*; D051381:Rats
D009124:Muscle Proteins; D010446:Peptide Fragments; D011498:Protein Precursors; D009320:Atrial Natriuretic Factor; D003488:Cyanogen Bromide