Sectional analysis of tumor colony growth in the soft-agar assay: effects of oxygen.
Soft-agar clonogenicity of L1210 mouse leukemia cells and of xenografts of a human melanoma and a carcinoma of the cervix was studied sectionally by the sizes of the colonies grown under hypoxic gradients and aerobic condition. Soft-agar plating efficiency was increased in cultured L1210 cells with decreasing oxygen concentrations. The growth of both cultured L1210 cells and their BDF1 ascites was better in 5% oxygen than in 20% oxygen. Although soft-agar colony development of both melanoma and cervical carcinoma was significantly better in 5% oxygen, the former has a secondary preference for a hypoxic atmosphere and the latter, for an aerobic condition.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D000860:Hypoxia; D007939:Leukemia L1210 / Q000473:pathology; D008545:Melanoma / Q000473:pathology; D051379:Mice; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000473:pathology