Action of creatinol-O-phosphate on the contractility changes evoked by hypoxia and ischemia in rat isolated heart.
The effect of creatinol-O-phosphate (N-methyl-N-(beta-hydroxyethyl)guanidine O-phosphate, creatinolfosfate, Aplodan) pretreatment has been studied on the recovery of contractility of rat isolated heart after hypoxia or ischemia. In normoxia creatinol-O-phosphate (100 mumol/l) evoked a positive inotropic effect only when glucose was present in the physiological solution, it also evoked a slight negative chronotropic effect that was independent of glucose. When creatinol-O-phosphate was present during hypoxia, in the physiological solution, the recovery of the contraction after reoxygenation (in the absence of the drug) was improved in a dose-dependent manner. When creatinol-O-phosphate was present in the physiological solution before ischemia, the recovery of the contractility after reperfusion was higher than in controls; the presence of creatinol-O-phosphate during reperfusion after ischemia accelerated the recovery of contractility. The action of creatinol-O-phosphate on the recovery of cardiac contractility after ischemia was also observed in hearts partially protected with a cardioplegic solution. It is suggested that creatinol-O-phosphate could exert its cardioprotective effect by an action on anaerobic glycolysis.
D000818:Animals; D003401:Creatine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D005260:Female; D005947:Glucose / Q000494:pharmacology; D006321:Heart / Q000187:drug effects*; D066298:In Vitro Techniques; D009200:Myocardial Contraction / Q000187:drug effects*; D010100:Oxygen / Q000494:pharmacology; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
C100173:creatinol phosphate; D005947:Glucose; D003401:Creatine; D010100:Oxygen