A 12 S precursor to 5.8 S rRNA associated with rat liver nucleolar 28 S rRNA.
The pre-rRNA and rRNA components of rat and mouse liver nucleolar RNA were analysed. It was shown that upon denaturation, part of the 32 S pre-rRNA is converted into 28 S rRNA and 12 S RNA. The 12 S RNA from mouse (Mr, 0.36 X 10(6)) is larger than the one from rat (Mr, 0.32 X 10(6). The 12 S RNA chain is intact and resists denaturation treatment. The non-covalent binding of this RNA with nucleolar 28 S rRNA is stronger than that of 5.8 S rRNA with 28 S rRNA. Hybridization with a rat internal-transcribed spacer rDNA fragment identifies 12 S RNA as corresponding to the 5'-end non-conserved segment of 32 S pre-rRNA, including 5.8 S rRNA. The significance of the formation of a 12 S precursor to 5.8 S rRNA in the biogenesis of ribosomes in mammalian cells is discussed.
D000818:Animals; D002466:Cell Nucleolus / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009694:Nucleic Acid Precursors / Q000235:genetics* / Q000302:isolation & purification; D012322:RNA Precursors; D012335:RNA, Ribosomal / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D009694:Nucleic Acid Precursors; D012322:RNA Precursors; D012335:RNA, Ribosomal