[Effect of beta adrenergic drugs on phospholipid synthesis in the human fetal lung].
Human fetal lungs originating from legal absortion (n:16) (8-12 weeks) were cultured for 3 weeks. The effects of beta-adrenergic and/or corticosteroid stimulation on the synthesis of osmiophilic lamellar bodies i.e. surfactant production in II type pneumocytes were studied. Fenoterol (10 ng/5 ml for 5 days) has no effect on the synthesis of lamellar bodies. Following dexamethasone treatment (10 ng/5 ml for 5 days) characteristic osmiophilic lamellar bodies appeared in the cytoplasm. If fenoterol and dexamethasone were applied together in the culture altered lamellar body structure could have been observed: the lamellar bodies had a large homogenous osmiophilic center and some parts of the osmiophilic material were found outside the lamellar bodies. It seems that the beta-adrenergic stimulation does not induce lamellar body synthesis, but it can play a role in the release of surfactant from the lamellar bodies into cytoplasm and alveolar space as well.
D046508:Culture Techniques; D003907:Dexamethasone / Q000494:pharmacology; D004983:Ethanolamines / Q000494:pharmacology*; D005260:Female; D005280:Fenoterol / Q000494:pharmacology*; D005325:Fetal Organ Maturity / Q000187:drug effects*; D006801:Humans; D008168:Lung / Q000187:drug effects* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010743:Phospholipids / Q000096:biosynthesis*; D011247:Pregnancy; D011663:Pulmonary Surfactants / Q000096:biosynthesis
D004983:Ethanolamines; D010743:Phospholipids; D011663:Pulmonary Surfactants; D005280:Fenoterol; D003907:Dexamethasone
Die Wirkung von Beta-Adrenergika auf die Phospholipidsynthese der menschlichen fetalen Lunge.