Down-regulation of glucocorticoid receptors in mouse lymphoma cell variants.
The mouse thymoma-derived cell line W7 is sensitive to the cytolytic action of glucocorticoids. We have isolated a novel class of cell variant that apparently overcomes its inherent sensitivity to glucocorticoids by reversibly down-regulating the level of glucocorticoid receptors. This phenotype is stable during subcloning in the presence and in the absence of glucocorticoids and is dominant in somatic cell hybrids with wild-type cells. Fusion of this variant with wild-type cells produces hybrids that down-regulate and are less sensitive to glucocorticoids than hybrids of receptor-negative and wild-type cells. This is the first demonstration of a phenotypic change which correlates with down-regulation of the glucocorticoid receptor.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003907:Dexamethasone / Q000378:metabolism / Q000494:pharmacology; D014644:Genetic Variation; D006822:Hybrid Cells / Q000187:drug effects / Q000502:physiology; D008228:Lymphoma, Non-Hodgkin / Q000503:physiopathology*; D051379:Mice; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D013945:Thymoma / Q000503:physiopathology*; D013953:Thymus Neoplasms / Q000503:physiopathology*
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D003907:Dexamethasone