Evidence for the existence of procolipase in chicken pancreas and pancreatic juice.
Purified antibodies raised against chicken colipase were coupled to Sepharose 4B and colipase was isolated in a single step by immunoaffinity chromatography from an extract of chicken pancreas prepared under conditions where trypsin activation is avoided. The purified protein has a single amino terminal residue of alanine and its biochemical properties are similar to those of the precursor form of colipase (procolipase) previously isolated from porcine and equine pancreas or pancreatic juice. Further evidence for the existence of procolipase was obtained from kinetic studies of the hydrolysis of the Intralipid emulsion by untreated and trypsin-treated chicken pancreatic juice.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D002645:Chickens; D002846:Chromatography, Affinity; D003089:Colipases / Q000302:isolation & purification*; D004792:Enzyme Precursors; D010179:Pancreas / Q000032:analysis*; D010189:Pancreatic Juice / Q000032:analysis*; D011498:Protein Precursors / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D003089:Colipases; D004792:Enzyme Precursors; D011498:Protein Precursors; D011506:Proteins; C037107:procolipase