Expression of mRNAs microinjected into somatic cells.
The microinjection of macromolecules into living somatic cells has been used to assess the biological activity of a wide variety of macromolecules. This article describes and evaluates the methodology of microinjection with micropipettes and reviews information on the expression of globin mRNAs injected into normal and transformed cultured cells. A brief account is also given of other mRNAs that have been successfully translated in cultured cells.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D005914:Globins / Q000235:genetics; D007980:Levodopa / Q000378:metabolism; D008543:Melanins / Q000235:genetics; D008545:Melanoma / Q000378:metabolism; D051379:Mice; D008845:Microinjections / Q000295:instrumentation / Q000379:methods; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*
D008543:Melanins; D012333:RNA, Messenger; D007980:Levodopa; D005914:Globins