title: Transformation of leukotriene A4 methyl ester to leukotriene C4 monomethyl ester by cytosolic rat glutathione transferases.
abstract: Six major basic cytosolic glutathione transferases from rat liver catalyzed the conversion of leukotriene A4 methyl ester to the corresponding leukotriene C4 monomethyl ester. Glutathione transferase 4-4, the most active among these enzymes, had a Vmax of 615 nmol X min-1 X mg protein-1 at 30 degrees C in the presence of 5 mM glutathione. It was followed in efficiency by transferase 3-4 which had a Vmax of 160 nmol X min-1 X mg-1 under the same conditions. Transferases 1-1, 1-2, 2-2 and 3-3 had at least 30 times lower Vmax values than transferase 4-4.
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology; D005982:Glutathione Transferase / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D017572:Leukotriene A4 / Q000031:analogs & derivatives*; D017997:Leukotriene C4 / Q000031:analogs & derivatives*; D008099:Liver / Q000201:enzymology*; D051381:Rats; D013189:SRS-A / Q000031:analogs & derivatives* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D014316:Tritium
chemical_list: D001095:Arachidonic Acids; D007527:Isoenzymes; D017572:Leukotriene A4; D013189:SRS-A; D014316:Tritium; D017997:Leukotriene C4; C043074:leukotriene C4 monomethyl ester; C043073:leukotriene A methyl ester; D005982:Glutathione Transferase