Inhibition of aspartate aminotransferase by hydrazinosuccinate.
DL-Hydrazinosuccinic acid was synthesized by the reaction of DL-bromosuccinic acid with hydrazine. The compound strongly inhibited aspartate aminotransferase and gave 50% inhibition at 1.3 microM when added simultaneously with L-aspartate to an assay mixture containing enzyme. Incubation of the enzyme with the compound prior to assay resulted in a much stronger inhibition, which proceeded time-dependently. The inhibition was protectable with L-aspartate and was substantially reversed by dialysis.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000037:antagonists & inhibitors*; D006834:Hydrazines / Q000138:chemical synthesis / Q000494:pharmacology*; D007202:Indicators and Reagents; D007700:Kinetics; D009206:Myocardium / Q000201:enzymology; D013386:Succinates; D013552:Swine
D006834:Hydrazines; D007202:Indicators and Reagents; D013386:Succinates; C029424:hydrazine; C042747:hydrazinosuccinic acid; D001219:Aspartate Aminotransferases; C033594:bromosuccinic acid