Cytoxicity of erythrocyte ghosts loaded with ribosome-inactivating proteins following fusion with CHO cells.
The ribosome-inactivating proteins gelonin, Momordica charantia inhibitor, pokeweed antiviral protein, and one from Saponaria officinalis were enclosed in human erythrocyte ghosts. The proteins once trapped in ghosts and fused with CHO cells inhibited colony formation at concentrations of approximately 1 ng/ml (3 X 10(-11) M), whereas the free proteins only had an effect at concentrations of greater than 1 microgram/ml.
D000818:Animals; D000998:Antiviral Agents / Q000633:toxicity*; D002459:Cell Fusion / Q000187:drug effects*; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004910:Erythrocyte Membrane / Q000502:physiology*; D005260:Female; D006801:Humans; D007700:Kinetics; D009699:N-Glycosyl Hydrolases; D010053:Ovary; D010940:Plant Proteins / Q000633:toxicity*; D014176:Protein Biosynthesis / Q000187:drug effects; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D054789:Ribosome Inactivating Proteins, Type 1
D000998:Antiviral Agents; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; C025674:GEL protein, Gelonium multiflorum; D009699:N-Glycosyl Hydrolases; C024275:pokeweed antiviral protein