Selective in vitro toxicity of purothionin conjugated to the monoclonal antibody 225.28S to a human high-molecular-weight melanoma-associated antigen.
The toxic agent purothionin was conjugated to the monoclonal antibody 225.28S to a human high-molecular-weight melanoma-accociated antigen. The toxic conjugate displayed in vitro toxicity to cultured human Colo 38 melanoma cells as indicated by reduced uptake of 3H-thymidine following a 24-h incubation and loss of cell viability following a 7-day incubation. The effect is dose-dependent and is specific since addition of the toxic conjugate to a cultured Raji B lymphoid cells did not affect their 3H-thymidine uptake or their viability.
D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D023181:Antimicrobial Cationic Peptides; D002470:Cell Survival; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D008545:Melanoma / Q000276:immunology* / Q000628:therapy; D008970:Molecular Weight; D010940:Plant Proteins / Q000008:administration & dosage*; D013205:Staphylococcal Protein A
D000911:Antibodies, Monoclonal; D023181:Antimicrobial Cationic Peptides; D010940:Plant Proteins; D013205:Staphylococcal Protein A; D004247:DNA; C016085:purothionin