Synaptosomal localization and release of glucagon-like materials in the rat brain.
The subcellular localization of glucagon-like materials in the thalamus-hypothalamus and brain stem of the rat was investigated. Both glucagon immunoreactivity (GI) determined by C-terminal specific antibody and glucagon-like immunoreactivity (GLI) determined by non-specific antibody were enriched in the microsomal and synaptosomal fractions relative to the nuclear, myelin and mitochondrial fractions. Furthermore, the synaptosomal fraction of both the thalamus-hypothalamus and brain stem incubated in Krebs-Ringer bicarbonate buffer with 55 mM K+ at 37 degrees C released GI and GLI in the presence of Ca++. These findings suggested that glucagon-like substances detected in the brain have a role in the synaptic function.
D000818:Animals; D001923:Brain Chemistry; D001933:Brain Stem / Q000032:analysis; D005934:Glucagon / Q000032:analysis*; D004763:Glucagon-Like Peptides; D007031:Hypothalamus / Q000032:analysis; D008297:Male; D008861:Microsomes / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000032:analysis; D013574:Synaptosomes / Q000032:analysis; D013788:Thalamus / Q000032:analysis
D010455:Peptides; D004763:Glucagon-Like Peptides; C015236:glucagon-like-immunoreactivity; D005934:Glucagon