Onchocerca gibsoni: increase of circulating egg antigen with chemotherapy in bovines.
Monoclonal antibodies directed to stage-specific surface antigens of Onchocerca gibsoni eggs were used in immunoradiometric assays to detect antigens in the sera of cattle infected with O. gibsoni. Two monoclonal antibodies detected antigens, presumably of egg origin, in sera. The target antigens appeared to be carbohydrate in nature and of variable molecular weights. Significant increases in levels of circulating egg antigens were found after treatment of infected cattle with benzimidazole compounds. These drugs cause disruption of embryogenesis and accelerated loss of worm uterine contents. In contrast, administration of either macrofilaricides or microfilaricides to infected cattle did not alter pretreatment levels of circulating egg antigens. Measurement of changes in levels of circulating antigens by immunoradiometric assays with stage-specific monoclonal antibodies provides a new means of assessing the efficacy of drugs and their site of action in onchocerciasis.
D000818:Animals; D000871:Anthelmintics / Q000627:therapeutic use*; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D002417:Cattle; D004049:Diethylcarbamazine / Q000627:therapeutic use; D005260:Female; D005369:Filaricides / Q000627:therapeutic use*; D017879:Isothiocyanates; D007559:Ivermectin; D007783:Lactones / Q000627:therapeutic use; D008297:Male; D008463:Mebendazole / Q000627:therapeutic use; D009854:Onchocerca / Q000187:drug effects / Q000196:embryology / Q000276:immunology*; D009855:Onchocerciasis / Q000188:drug therapy* / Q000276:immunology / Q000469:parasitology; D010063:Ovum / Q000276:immunology*; D010879:Piperazines / Q000627:therapeutic use; D011223:Praziquantel / Q000627:therapeutic use; D013861:Thiocyanates / Q000627:therapeutic use
D000871:Anthelmintics; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D005369:Filaricides; D017879:Isothiocyanates; D007783:Lactones; D010879:Piperazines; D013861:Thiocyanates; D011223:Praziquantel; D007559:Ivermectin; D008463:Mebendazole; C042068:CGA 110755; C042069:CGA 114635; D004049:Diethylcarbamazine