Immunospecific binding of tRNA precursors containing N6-(delta 2-isopentenyl) adenosine.
Antibodies specific for N6-(delta 2-isopentenyl) adenosine (i6A) were immobilized on Sepharose and this adsorbent (Sepharose-anti-i6A) was used to selectively isolate bacteriophage T4 tRNA precursors containing i6A/ms2i6A from an unfractionated population of 32P-labeled T4 RNAs. The results showed that antibodies to i6A selectively bound only those tRNA precursors containing i6A/ms2i6A. Binding of tRNA precursors by antibody and specificity of the binding was assessed by membrane binding using 32P-labeled tRNA precursor. Binding was highly specific for i6A/ms2i6A residues in the tRNA precursors. This binding can be used to separate modified from unmodified precursor RNAs and to study the biosynthetic pathways of tRNA precursors.
D000225:Adenine / Q000031:analogs & derivatives* / Q000032:analysis; D000906:Antibodies; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D007541:Isopentenyladenosine; D009694:Nucleic Acid Precursors / Q000302:isolation & purification*; D012322:RNA Precursors; D012343:RNA, Transfer / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification; D011863:Radioimmunoassay; D013604:T-Phages
D000906:Antibodies; D009694:Nucleic Acid Precursors; D012322:RNA Precursors; D012367:RNA, Viral; C001478:N(6)-(delta(2)-isopentenyl)adenine; D007541:Isopentenyladenosine; D012343:RNA, Transfer; D000225:Adenine