In vitro development of porcine embryos in coculture with endometrial cell monolayers or culture supernatants.
The objective of this study was to determine if porcine endometrial cell contact would enhance the in vitro development of porcine embryos relative to endometrial cell culture supernatants or ovarian fibroblast contact. One hundred forty-nine embryos of various developmental stages were collected from 10 excised gilt reproductive tracts. Embryos were washed three times and placed randomly into one of four treatments: Minimal Essential Medium + 5% heat-treated fetal calf serum (complete MEM), porcine endometrial cell coculture (pEM), porcine endometrial cell supernatant (pEMS) and porcine ovarian fibroblast coculture (pOF). All cultures were conducted in 96 well microtiter plates, one embryo per well, at 37 C in a 5% CO2 in humidified air atmosphere. Observations for stage of development were made every 12 h by phase contrast microscopy (100X). Embryos in the pEM and pOF treatments had significantly greater (P less than .05) mean embryonic development scores (1.59 +/- .22 and 1.44 +/- .18, respectively) than embryos cultured in complete MEM only or pEMS (.63 +/- .13 and .48 +/- .15, respectively). The results of this study indicate that cell to embryo contact enhances in vitro development of porcine embryos.
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004622:Embryo, Mammalian / Q000648:ultrastructure; D005314:Embryonic and Fetal Development; D004717:Endometrium / Q000502:physiology*; D005260:Female; D005347:Fibroblasts / Q000502:physiology; D066298:In Vitro Techniques; D010053:Ovary / Q000166:cytology; D013552:Swine / Q000196:embryology*; D015044:Zona Pellucida / Q000648:ultrastructure
D003470:Culture Media