Determination of immunoreactive trypsin, pancreatic elastase and chymotrypsin in extracts of human feces and ileostomy drainage.
The total daily amount of extractable cationic trypsin, chymotrypsin, and pancreatic elastase 2 in feces and ileostomy fluids has been studied in normal individuals and healthy colectomized subjects. Quantitation was performed using immunological assays with polyethylene glycol as a fecal marker. The extractable amount of each of these enzymes in the feces of normal individuals was less than 1 mg/24 h. However, in fecal extracts from antibiotic-treated normal individuals a 100-fold increase in immunoreactive cationic trypsin was observed, while chymotrypsin and elastase 2 were only 2- to 3-fold higher. In extracts from ileostomy fluids cationic trypsin, elastase, and chymotrypsin all showed mean values in the order of 50-200 mg/24 h. The characterization of the immunoreactivity of pancreatic proteases showed no qualitative differences when measured in duodenal juice or fecal and ileostomy extracts.
D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001826:Body Fluids / Q000201:enzymology; D002850:Chromatography, Gel; D002918:Chymotrypsin / Q000032:analysis*; D003106:Colon / Q000187:drug effects; D003451:Cryoprotective Agents; D005243:Feces / Q000201:enzymology*; D006801:Humans; D007081:Ileostomy; D008875:Middle Aged; D010196:Pancreatic Elastase / Q000032:analysis*; D011092:Polyethylene Glycols; D011863:Radioimmunoassay; D014357:Trypsin / Q000032:analysis*
D000900:Anti-Bacterial Agents; D003451:Cryoprotective Agents; D011092:Polyethylene Glycols; D002918:Chymotrypsin; D010196:Pancreatic Elastase; D014357:Trypsin