title: Rapid isolation of endogenous inhibitor of factor B.
abstract: An inhibitor of mouse factor B was purified from lysate of L cells by a human factor B-affinity column. The purified inhibitor was found to be homogeneous with a molecular weight of about 25,000 and identical to the 25K protein isolated from L cell-lysate by the coprecipitation method with anti mouse factor B. Hemolytic titration of the activity and PAGE-analysis of the inhibitor-factor B complex showed that 1 mol of inhibitor reacted with 1 mol of mouse factor B and inactivated its hemolytic activity.
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D011415:Complement Factor B / Q000037:antagonists & inhibitors*; D004792:Enzyme Precursors / Q000037:antagonists & inhibitors*; D006461:Hemolysis; D006801:Humans; D007739:L Cells / Q000032:analysis; D051379:Mice; D008970:Molecular Weight
chemical_list: D004792:Enzyme Precursors; D011415:Complement Factor B