Isolation and structures of the third major type of carbohydrate units in polysialoglycoproteins from rainbow trout eggs.
The structures of the third major type of oligosialyloligosaccharides obtained by alkali-borohydride treatment of polysialoglycoproteins of the unfertilized eggs of rainbow trout have been determined to be: alpha-L-fucosyl-(1----3)-beta-N-acetyl-D-galactosaminyl-(1----3)-beta-D- galactosyl-(1----4)-beta-D-galactosyl-(1----3)-[[(----8)-alpha-N- glycolylneuraminyl-(2----)]n-(----6)]-N-acetyl-D-galactosaminitol with n = 1 to 4. The proportion of the fucose-containing units in 3 major carbohydrate units present in fish egg polysialoglycoproteins was found to be highly species-specific and only 6% in the eggs of rainbow trout.
D000818:Animals; D002240:Carbohydrate Sequence; D005260:Female; D005643:Fucose / Q000032:analysis; D010063:Ovum / Q000032:analysis*; D012487:Salmonidae / Q000196:embryology*; D012795:Sialoglycoproteins / Q000032:analysis*; D014337:Trout / Q000196:embryology*
D012795:Sialoglycoproteins; D005643:Fucose