Cellular localization of binding sites for 3H-DSIP on neurones of cultured rat brain stem.
By means of light-microscopic autoradiography, binding sites for the delta sleep-inducing peptide (3H-DSIP) were observed on the soma and processes of cultured brain stem neurones but not on glial cells. Unlabelled DSIP at high concentrations inhibited binding of 3H-DSIP, indicating specific binding of this peptide. Our data suggest that brain stem neurones might possess receptors for the sleep-inducing peptide.
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D001933:Brain Stem / Q000378:metabolism*; D046508:Culture Techniques; D003701:Delta Sleep-Inducing Peptide; D005333:Fetus; D009842:Oligopeptides / Q000378:metabolism*; D051381:Rats
D009842:Oligopeptides; D003701:Delta Sleep-Inducing Peptide