Neuromedin N: a novel neurotensin-like peptide identified in porcine spinal cord.
A novel neurotensin-like peptide, designated neuromedin N, has been isolated from porcine spinal cord by using a bioassay for a stimulant effect on guinea pig ileum. By microsequencing, the amino acid sequence of the peptide has been determined to be Lys-Ile-Pro-Tyr-Ile-Leu, which is found to be quite homologous to the COOH-terminal sequence of neurotensin. This structure has been confirmed by synthesis. Neuromedin N exhibits a contractile activity on guinea pig ileum and induces a hypotensive response in the rat similar to that with neurotensin. These findings suggest that neuromedin N may be a new neuromediator or hormone with a specific spectrum of biological activity.
D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D001794:Blood Pressure / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D009119:Muscle Contraction / Q000187:drug effects; D009496:Neurotensin / Q000302:isolation & purification* / Q000494:pharmacology; D010446:Peptide Fragments / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D013116:Spinal Cord / Q000032:analysis*; D013329:Structure-Activity Relationship; D013552:Swine
D010446:Peptide Fragments; C042090:neuromedin N; D009496:Neurotensin