Sequence and structure of a serine transfer RNA with GCU anticodon from mosquito mitochondria.
We have determined the primary sequence and modification status of a transfer RNA from mosquito mitochondria whose GCU anticodon indicates that it is a serine tRNA (tRNASerGCU), and have obtained information on higher order structure using partial digestion with nucleases S1 and T1 under non-denaturing conditions. Although its primary sequence homology to mammalian mitochondrial tRNASerGCU is modest (46%), the mosquito tRNA resembles its mammalian mitochondrial counterpart in that a plausible secondary structure configuration includes a drastically abbreviated D arm and a sex base-pair anticodon stem. Other unusual features include a ribose-methylated cytidine residue at the end of the anticodon stem, and the likely occurrence of a psi residue between the amino acid arm and arm IV.
D000330:Aedes / Q000032:analysis / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D008928:Mitochondria / Q000032:analysis; D009690:Nucleic Acid Conformation; D012346:RNA, Transfer, Amino Acyl
D012346:RNA, Transfer, Amino Acyl