[Results of the transplantation of 2 lines of human endometrial adenocarcinoma into the nude mouse: takes of the graft and true tumors].
Two human endometrial adenocarcinoma cell lines, isolated and routinely cultivated in our laboratory were transplanted into nude mice. MOQ cells, isolated in 1979, are devoid of estrogen (E) receptors; GUS cells, isolated in 1981, are bearing E and progesterone receptors. Cell inoculation was realized in 504 nude mice in various conditions (male or female mice, pre or post puberal, normal or castrated, with or without E supplementation). The aim of these experiments was to establish cell tumorigenicity and to compare growth characteristics in vivo and in vitro. Our results show that the age of the mice and of the cells is important for the "take" of the injected cells. Young mice seem to possess thymo-independent graft resistance stronger than old mice; "young cells", near re-cultivation from a nude mice tumour, are more tumorigenic than "old" cells, always cultivated in vitro from the time of first explantation. There is also evidence that E improve the "take" of the cells in the two cell lines, regardless of the presence of E receptors. This is suggesting of an indirect action of E on tumour growth. Finally, the most striking result is the fact that castration is the decisive factor leading to true tumours, suggesting direct growth stimulation by hypophyseal factors.
D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D002460:Cell Line; D005260:Female; D006085:Graft Survival; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014594:Uterine Neoplasms / Q000473:pathology*
Résultats de la transplantation de deux lignées d'adénocarcinome endométrial humain à la souris nue: prises de greffe et tumeurs vraies.