Characterization of new enkephalin-containing peptides in the adrenal medulla by immunoblotting.
Immunoblotting combined with radioimmunoassays (RIAs) directed specifically towards certain sequences of the proenkephalin molecule has been used to characterize the enkephalin-containing peptides (ECPs) present in the bovine adrenal medulla. Immunoblotting allowed the simultaneous visualization of all ECPs present in a crude extract of this gland. Combining this technique with RIAs we have been able to characterize a new high molecular mass ECP, a 23.3-kDa protein which contains the amino-terminal part of proenkephalin and ends with the sequence of Leu-enkephalin at its carboxy-terminus.
D000313:Adrenal Medulla / Q000032:analysis*; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D004743:Enkephalin, Leucine / Q000276:immunology; D004744:Enkephalin, Methionine / Q000276:immunology; D004745:Enkephalins / Q000032:analysis* / Q000276:immunology; D007106:Immune Sera; D007122:Immunoelectrophoresis; D008970:Molecular Weight; D011498:Protein Precursors / Q000276:immunology; D011863:Radioimmunoassay
D004745:Enkephalins; D007106:Immune Sera; D011498:Protein Precursors; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; C036693:synenkephalin