Inhibition of calcium sequestration activity of liver microsomes by 4-hydroxyalkenals originating from the peroxidation of liver microsomal lipids.
Aldehydes released during peroxidation of liver microsomal lipids and identified as 4-hydroxyalkenals (4-hydroxynonenal being quantitatively the most significant) strongly inhibited the calcium sequestration activity of liver microsomes. The ID50 for 4-hydroxynonenal was 42 microM. The inhibition appeared to be correlated with the amount of the aldehyde bound to the microsomal protein. In rats intoxicated with BrCCl3 significant amounts of protein-bound aldehydes were formed at only 5 min after poisoning, a time at which the calcium sequestring capacity is markedly inhibited.
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D001975:Bromotrichloromethane / Q000506:poisoning; D002118:Calcium / Q000378:metabolism*; D002451:Cell Compartmentation / Q000187:drug effects; D008054:Lipid Peroxides / Q000494:pharmacology; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats
D000447:Aldehydes; D008054:Lipid Peroxides; D001975:Bromotrichloromethane; C027576:4-hydroxy-2-nonenal; D002118:Calcium