[Primary structure of tRNA2Leu from the bovine udder. Determination of the oligonucleotide structure of the pyrimidyl-RNAse hydrolysate of tRNA2Leu by microspectrophotometric methods].
tRNA2Leu from cow mammary gland has been degraded with pancreatic ribonuclease, and the fragments obtained were separated by DEAE-cellulose micro-column chromatography in 7 M urea at pH 7,5. Rechromatography was performed on a DEAE-cellulose micro-column at pH 3,7 and also on Dowex 1 X 2 in a formiate system. Nucleotide analysis was carried out with the aid of T2-RNase hydrolysis followed by chromatography on anion-exchanger AG 1 X 8. Nucleosides were separated on Aminex A-6 at pH 9,8. 15 minor components were shown to be present: T, 2 psi, 2Um, 2D, m5C, ac4C, m1G, 2m2G, m22G, m1A and N, the N is not identified so far. The structure of oligonucleotides was established by terminal analysis, hydrolysis with T1-RNase and also using incomplete hydrolysis by the snake venom phosphodiesterase.
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D005260:Female; D006868:Hydrolysis; D008321:Mammary Glands, Animal / Q000032:analysis*; D009841:Oligonucleotides / Q000032:analysis*; D009843:Oligoribonucleotides / Q000032:analysis*; D012346:RNA, Transfer, Amino Acyl / Q000032:analysis*; D012260:Ribonucleases / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D009841:Oligonucleotides; D009843:Oligoribonucleotides; D012346:RNA, Transfer, Amino Acyl; D012260:Ribonucleases
Pervichnaia struktura tRNK2Leu molochnoĭ zhelezy korov. Opredelenie struktury oligonukleotidov pirimidil-RNKaznogo gidrolizata tRNK2Leu mikrospektrofotometricheskimi metodami.