Relation between red cell anion exchange and water transport.
A new distilbene compound, 4',4'-dichloromercuric-2,2,2',2'-bistilbene tetrasulfonic acid (DCMBT), has been synthesized for use in studies of anion and water transport in the human red cell. DCMBT combines features of both the specific stilbene anion transport inhibitor, DIDS, and the mercurial water transport inhibitor, pCMBS. This new compound inhibits anion transport almost completely with a Ki of 15 microM. DCMBT also inhibits water transport by about 15-20% with a Ki of about 8 microM. Treatment of red cells with DIDS inhibits the effect of DCMBT on water transport, suggesting that anion transport and water transport are mediated by the same protein.
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D027321:Anion Transport Proteins; D001693:Biological Transport, Active / Q000187:drug effects; D001834:Body Water / Q000378:metabolism*; D002352:Carrier Proteins / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009941:Organomercury Compounds / Q000494:pharmacology*; D013267:Stilbenes / Q000494:pharmacology*; D013431:Sulfates / Q000097:blood; D013997:Time Factors
D027321:Anion Transport Proteins; D002352:Carrier Proteins; D009941:Organomercury Compounds; D013267:Stilbenes; D013431:Sulfates; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; C043803:4',4'-dichloromercuric-2,2,2',2'-bistilbene tetrasulfonic acid; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid