A phosphorus NMR study of the reaction products of cis-diamminedichloroplatinum (II) with a double-helical oligonucleotide and with DNA.
The structural distortion of oligonucleotides upon cis-PtCl2(NH3)2[d(T-C-T-C-G-G-T-C-T-C)-N7(5), N7(6)] reveals shifting of 4 phosphorus resonances due to platination. 3 Resonances could be assigned by selective 31P-irradiation, showing P(6) (P between the two Gs) to be shifted 1.5 ppm to low field. In the concomitant double strands P(6) is shifted 0.9 ppm to lower field. A similar peak has been observed in platinated salmon sperm DNA (37 degrees C), indicating that Pt-binding to GpG-fragments in DNA is similar to that found for the decanucleotide, so the distortion of DNA might be comparable.
D000818:Animals; D002945:Cisplatin / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009841:Oligonucleotides / Q000378:metabolism*; D010758:Phosphorus; D012474:Salmon; D013094:Spermatozoa / Q000032:analysis
D009841:Oligonucleotides; D010758:Phosphorus; D004247:DNA; D002945:Cisplatin