Glucocorticoid-dependent uteroglobin synthesis and uteroglobulin mRNA levels in rabbit lung explants cultured in vitro.
In rabbit lung explants cultured in vitro in a synthetic medium, the synthesis of the protein uteroglobin decayed progressively becoming virtually undetectable between 24-48 h of culture. Addition of glucocorticoids to the medium maintained the synthesis of uteroglobin. This glucocorticoid effect was dose-dependent with optima at about 0.1 microM and 1 microM for dexamethasone and cortisol respectively. Estradiol, progesterone, triiodothyronine, insulin or 10% calf serum added to the medium were ineffective in maintaining uteroglobin synthesis. Actinomycin D (10 micrograms/ml) added to the medium inhibited the effect of cortisol on uteroglobin synthesis. After 24 h of culture, both the relative levels of uteroglobin mRNA, measured by molecular hybridization, and uteroglobin synthesis were correlatively higher (up to 10-fold) in glucocorticoid-treated than in control explants.
D000818:Animals; D004247:DNA / Q000032:analysis; D003609:Dactinomycin / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology*; D006023:Glycoproteins / Q000096:biosynthesis*; D006854:Hydrocortisone / Q000494:pharmacology*; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D009924:Organ Culture Techniques; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D013997:Time Factors; D014598:Uteroglobin / Q000096:biosynthesis*
D006023:Glycoproteins; D012333:RNA, Messenger; D003609:Dactinomycin; D003907:Dexamethasone; D004247:DNA; D014598:Uteroglobin; D006854:Hydrocortisone