Butyl-Toyopearl 650 as a new hydrophobic adsorbent for water-soluble enzyme proteins.
Butyl-Toyopearl 650, a butyl derivative of Toyopearl HW-65, was synthesized for use in hydrophobic chromatography. Water-soluble enzyme proteins were adsorbed on butyl-Toyopearl 650 in the presence of ammonium sulfate and eluted easily in the absence of the salt. Cytochrome c, myoglobin, and chymotrypsinogen A were successfully separated on a butyl-Toyopearl 650 column in order of their individual hydrophobicity by decreasing the concentration of ammonium sulfate contained in the buffer eluant. Based on these results, the use of butyl-Toyopearl 650 is demonstrated for the hydrophobic separation of water-soluble enzyme proteins.
D000327:Adsorption; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography / Q000379:methods*; D004798:Enzymes / Q000032:analysis*; D011108:Polymers / Q000138:chemical synthesis; D012995:Solubility; D014867:Water
D004798:Enzymes; D011108:Polymers; D014867:Water; C041725:butyl-Toyopearl 650