Assay for Hageman factor (factor XII) in human plasma by means of chromogenic substrate for plasma kallikrein.
Determination of factor XII was performed in a test system where plasma samples were diluted (1/400-1/3200) in buffer and factor XII deficient plasma. Diluted CephotestR was used as activator and after 10 min activation time chromogenic substrate H-D-Pro-Phe-Arg-pNA was added. The assay was adopted to an enzyme analyzer. The reproducibility of the method was acceptable with a coefficient of variation of 7.8% in the normal range. The correlation of this method to a one-stage clotting assay for factor XII was good, r 0.79. The preliminary reference interval was calculated to be 52-147% of normal (mean+ 2 S.D.).
D002863:Chromogenic Compounds; D005174:Factor XII / Q000032:analysis*; D007610:Kallikreins / Q000378:metabolism*; D010949:Plasma / Q000032:analysis
D002863:Chromogenic Compounds; D005174:Factor XII; D007610:Kallikreins