Light intensities required to suppress nocturnal melatonin secretion in albino and pigmented rats.
Sprague-Dawley albino rats or Long-Evans pigmented rats were exposed during the dark phase of the daily light:dark cycle to various intensities of a sunlight-stimulating white fluorescent light (0.022, 0.044, 0.110, 0.220, 0.440 or 2.200 microW/cm2) for 30 min; pineal glands and trunk blood samples were then collected and assayed for melatonin by radioimmunoassay. Albino rats exposed to irradiances of 0.110 microW/cm2 or less had pineal melatonin levels that were not significantly different from those of unexposed animals; higher irradiances significantly (P less than 0.001) reduced melatonin levels. In contrast, as little as 0.022 microW/cm2 significantly (P less than 0.02) reduced pineal and serum melatonin levels in the pigmented rats. These results suggest that something other than the simple presence or absence of eye pigmentation is the critical factor in determining the sensitivity of the rat's pineal to retinal-mediated photic suppression of melatonin synthesis.
D000417:Albinism / Q000378:metabolism*; D000818:Animals; D002940:Circadian Rhythm; D008027:Light; D008297:Male; D008550:Melatonin / Q000378:metabolism*; D010870:Pineal Gland / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012880:Skin Pigmentation
D008550:Melatonin