title: Ribosomal subunit antiassociation activity in rabbit reticulocyte lysates. Evidence for a low molecular weight ribosomal subunit antiassociation protein factor (Mr = 25,000).
abstract: A ribosomal subunit antiassociation activity has been purified from both the postribosomal supernatant and ribosomal salt-wash protein fractions of rabbit reticulocyte lysates. A majority (greater than 90%) of the activity is associated with a low molecular weight protein of Mr of approximately 25,000. A small but significant level of antiassociation activity (less than 10%) was found to be associated with higher molecular weight protein fractions. The purified 25,000-dalton antiassociation factor interacts with 60 S ribosomal subunits to prevent them from reassociating with 40 S ribosomal subunits. The factor does not seem to interact directly with 40 S subunits nor does it dissociate 80 S monosomes. The properties of this factor are thus similar to the eukaryotic initiation factor 6 isolated from both wheat germ and calf liver extracts.
mesh_terms: D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D039621:Eukaryotic Initiation Factor-3; D008274:Magnesium / Q000378:metabolism; D008970:Molecular Weight; D010448:Peptide Initiation Factors / Q000032:analysis*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012358:RNA, Transfer, Met; D011817:Rabbits; D012156:Reticulocytes / Q000032:analysis*; D012269:Ribosomal Proteins / Q000032:analysis*
chemical_list: D039621:Eukaryotic Initiation Factor-3; D010448:Peptide Initiation Factors; D012346:RNA, Transfer, Amino Acyl; D012358:RNA, Transfer, Met; D012269:Ribosomal Proteins; C024988:tRNA, formylmethionine-; D008274:Magnesium