title: Comparison of high-performance liquid chromatography with electrochemical detection and gas chromatography-mass fragmentography for the assay of salsolinol, dopamine and dopamine metabolites in food and beverage samples.
abstract: High-performance liquid chromatography with electrochemical detection (HPLC-ED) and combined gas chromatography--mass spectrometry in the single-ion monitoring mode (GC--MS-SIM) have been used for the determination of salsolinol, dopamine, 3,4-dihydroxyphenylacetic acid, 3,4-dihydroxyphenylethanol and norepinephrine in a selection of food and beverage samples. The unique specificity of the SIM mode allows a simple one-step extraction to be used even for complex sample matrices. We have been able to demonstrate the quantitative and qualitative advantages offered by GC--MS over HPLC--ED by direct comparison of the chromatographic data obtained. We demonstrate that the specificity of SIM and the benefits offered by the incorporation of deuterated internal standards make GC--MS-SIM the method of choice for valid identification and precise quantitation of salsolinol, dopamine and dopamine metabolites in a complex sample matrix.
mesh_terms: D001515:Beer / Q000032:analysis; D001628:Beverages / Q000032:analysis*; D002395:Catecholamines / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004298:Dopamine / Q000032:analysis*; D004563:Electrochemistry; D005504:Food Analysis; D005638:Fruit / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007546:Isoquinolines / Q000032:analysis*; D013025:Glycine max / Q000032:analysis
chemical_list: D002395:Catecholamines; D007546:Isoquinolines; C036617:salsolinol; D004298:Dopamine