Effect of fibroblast-pneumonocyte factor on the synthesis of surfactant phospholipids in type II cells from fetal rat lung.
The effect of fibroblast-pneumonocyte factor on the formation of surfactant-associated phospholipids was studied in fetal rat lung type II cells in primary culture. Exposure of the cells to fibroblast-pneumonocyte factor enhanced the incorporation of radioactively labelled choline, glycerol and palmitate into total and saturated phosphatidylcholine. Fibroblast-pneumonocyte factor also stimulated the formation of phosphatidylglycerol from labelled glycerol and palmitate but did not significantly affect the synthesis of the other phospholipids. This indicates that fibroblast-pneumonocyte factor specifically stimulates the production of surfactant lipids in fetal lung.
D000818:Animals; D002478:Cells, Cultured; D002794:Choline / Q000378:metabolism; D005260:Female; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D005990:Glycerol / Q000378:metabolism; D008168:Lung / Q000196:embryology* / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis*; D011247:Pregnancy; D011663:Pulmonary Surfactants / Q000096:biosynthesis*; D051381:Rats
D009842:Oligopeptides; D010169:Palmitic Acids; D010743:Phospholipids; D011663:Pulmonary Surfactants; C044220:fibroblast pneumonocyte factor; D019308:Palmitic Acid; D005346:Fibroblast Growth Factors; D002794:Choline; D005990:Glycerol