Comparison of immune responses induced by rat RT-1 antigens presented as inserts into liposomes, as protein micelles and as intact cells.
Partially purified rat transplantation antigens (RT-1) were inserted into liposomes composed of various types of lipids and used for immunization. The immune responses induced by the liposomes were compared with responses induced by RT-1 as protein micelles, alone or emulsified in Freund's incomplete adjuvant, or intact cells. Liposomes gave generally a higher humoral response than protein micelles. Each type of RT-1 immunization gave a particular pattern of specific Ig (sub)class responses. Freund's incomplete adjuvant was not only lacking in potentiating effect on low protein dose immunization but had a significant inhibitory effect. Besides intact cells only distearoyl-phosphatidylcholine liposomes had the potential to induce a cell-mediated cytotoxic response.
D000818:Animals; D000917:Antibody Formation; D003102:Colloids; D003602:Cytotoxicity, Immunologic; D006649:Histocompatibility Antigens / Q000008:administration & dosage / Q000276:immunology*; D007136:Immunoglobulins / Q000096:biosynthesis / Q000145:classification; D008081:Liposomes / Q000276:immunology*; D008823:Micelles; D051381:Rats; D011914:Rats, Inbred BN; D011920:Rats, Inbred WF; D013154:Spleen / Q000276:immunology
D003102:Colloids; D006649:Histocompatibility Antigens; D007136:Immunoglobulins; D008081:Liposomes; D008823:Micelles; C038353:histocompatibility antigens RT, rat