Transport of glutathione by intestinal brush border membrane vesicles.
Intestinal brush border membranes prepared from pig jejunum were shown to transport intact reduced glutathione into an osmotically responsive intravesicular space. At early incubation time (under 1 min.) GSH was poorly hydrolysed in its free amino acids. Primarily glutathione uptake was obtained against a concentration gradient (vesicle greater than medium) and was stimulated by Na+.
D000818:Animals; D001692:Biological Transport; D003545:Cysteine / Q000031:analogs & derivatives*; D004151:Dipeptides; D005033:Ethylmaleimide / Q000031:analogs & derivatives; D005978:Glutathione / Q000378:metabolism*; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism*; D007700:Kinetics; D008871:Microvilli / Q000378:metabolism*; D009842:Oligopeptides; D009994:Osmolar Concentration; D013552:Swine
D004151:Dipeptides; D009842:Oligopeptides; C042944:N-ethylmaleimide-gamma-glutamyl-cysteinyl-glycine; C042945:N-ethylmaleimide-cysteine; C042946:N-ethylmaleimide-cysteinyl-glycine; D005978:Glutathione; D003545:Cysteine; D005033:Ethylmaleimide