A quantitative densitometric method for the rapid separation and quantitation of the major lipids of tissues and lipoproteins by high-performance thin-layer chromatography. II. Reduction of the densitometric data.
A BASIC program is described for acquisition of data and data reduction for an automated densitometric system for quantitation of lipids separated by high-performance thin-layer chromatography. The program allows calculation of mass of samples from log/log calibration curves computed from standards. The calculated masses are reported as nmol/volume or nmol/mg protein. The program contains a flexible dialog system which permits its use for a variety of applications in addition to the system described for quantitation of lipids.
D001322:Autoanalysis; D002855:Chromatography, Thin Layer / Q000295:instrumentation / Q000379:methods; D003201:Computers; D003720:Densitometry / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008055:Lipids / Q000032:analysis*; D008074:Lipoproteins / Q000032:analysis*; D012984:Software
D008055:Lipids; D008074:Lipoproteins