Inhibition of protein synthesis in mice by ochratoxin A and its prevention by phenylalanine.
The sensitivity of protein synthesis to ip doses of ochratoxin A ranging from 1 to 15 mg/kg body weight has been determined in the livers, kidneys and spleens of mice. The incorporation of 14C-labelled amino acids into protein was measured in the total tissue homogenate, in the 105,000-g supernatant fraction and in the fraction of thermostable soluble proteins. The inhibition of protein synthesis was greatest in spleen and kidney and least in liver. The percentage inhibition of protein synthesis by any one dose of ochratoxin A was similar in all of the protein fractions from any one organ. The degree of inhibition of protein synthesis 5 hr after administration of 1 mg ochratoxin A/kg was 26% in liver, 68% in kidney and 75% in spleen. Phenylalanine (100 mg/kg) injected together with ochratoxin A prevented the inhibition of protein synthesis by a dose of 10 mg OTA/kg in all of these organs.
D000818:Animals; D002250:Carbon Radioisotopes; D003907:Dexamethasone / Q000378:metabolism; D004347:Drug Interactions; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009793:Ochratoxins / Q000494:pharmacology*; D010649:Phenylalanine / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011492:Protein Hydrolysates / Q000378:metabolism; D013154:Spleen / Q000187:drug effects
D002250:Carbon Radioisotopes; D009793:Ochratoxins; D011492:Protein Hydrolysates; C025589:ochratoxin A; D010649:Phenylalanine; D003907:Dexamethasone