The lack of transformation activity of 9-hydroxybenzo[a]pyrene related to the absence of modified deoxyribonucleosides in DNA of C3H/10T1/2 cells.
Sephadex LH-20 chromatography of DNA digests of C3H/10T1/2 cells treated with [3H]9-hydroxybenzo[a]pyrene (9-OH-BaP) and [14C]BaP-7,8-diol showed: (i) the presence only of uncharacterized 3H radioactivity eluted in the early portion of the gradient; [3H]9-OH-BaP-4,5-oxide-modified deoxyribonucleosides were not observed. (ii) The total amount of [14C]-labelled radioactivity corresponded to nucleoside moieties which were modified by anti- and syn-benzo[a]pyrene diol-epoxide. The absence of nucleosides modified by 9-OH-BaP-4,5-oxide in C3H/10T1/2 cells observed in this study may account in part for the relative resistance of these cells to the mutagenic and transforming action of 9-OH-BaP.
D000818:Animals; D001580:Benzopyrenes / Q000494:pharmacology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004247:DNA / Q000032:analysis*; D004101:Dihydroxydihydrobenzopyrenes; D051379:Mice; D008809:Mice, Inbred C3H
D001580:Benzopyrenes; D004101:Dihydroxydihydrobenzopyrenes; C017228:benzo(a)pyrene 7,8-dihydrodiol; C021337:9-hydroxybenzo(a)pyrene; C042335:9-hydroxybenzo(a)pyrene-4,5-epoxide; D004247:DNA