[Incorporation of 1-0-alkyl-2-acetyl-sn-glycerol-3-phosphocholine into human plasma lipoproteins in vitro. Effect of the lecithin:cholesterol acyltransferase activity of plasma].
The binding of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine, a platelet activating factor (PAF), to individual classes of human plasma lipoproteins was studied. It was found that PAF is rapidly incorporated into plasma lipoproteins. The degree of PAF binding to the lipoproteins depends on their type. Analysis of PAF binding in Scatchard plots revealed that HDL2 contain high affinity binding sites for PAF. On the other hand, LDL bind PAF in a nonspecific manner. Incubation of PAF with human plasma results in inhibition of lecithin: cholesterol acyltransferase (LCAT)-catalyzed cholesterol esterification. On the contrary, prostaglandin E1 stimulates LCAT activity in human plasma. PAF and prostaglandin E1 do not compete with each other for the binding sites in HDL2. It is concluded that incorporation of PAF into plasma lipoproteins may influence the LCAT activity in vivo.
D000527:Alprostadil; D001667:Binding, Competitive; D002784:Cholesterol / Q000097:blood; D004951:Esterification; D006801:Humans; D066298:In Vitro Techniques; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood; D010972:Platelet Activating Factor / Q000378:metabolism*; D011458:Prostaglandins E / Q000378:metabolism; D011485:Protein Binding
D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D010972:Platelet Activating Factor; D011458:Prostaglandins E; D002784:Cholesterol; D007862:Phosphatidylcholine-Sterol O-Acyltransferase; D000527:Alprostadil
Vkliuchenie 1-O-alkil-2-atsetil-sn-glitsero-3-fosfokholina v lipoproteiny plazmy cheloveka in vitro. Vliianie na aktivnost' letsitin: kholesterin atsiltransferazy plazmy.