[Assay of urinary homovanillic acid by gas phase chromatography with a capillary tube].
A sensitive and specific method for the determination of urinary homovanillic acid by gas chromatography with a capillary silica fused flexible column, WCOT, SE 30 phase, is described. Homovanillyl alcohol is used as internal standard. OH and COOH functions are derivatized in a one step reaction with the mixture heptafluorobutyric anhydride - trifluoroethanol (4:1). A linear response of the detector (FID) is obtained with injected amounts of HVA ranging from 0 to more than 1000 ng (standard injected = 400 ng). The smallest detectable level of HVA is about 1 ng, corresponding to urinary levels near 0.1 mg/24 h, or 0.5 micromole/24 h. Correlations between this method and a fluorimetric determination of HVA are studied. Usual values in normal subjects are given.
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002849:Chromatography, Gas / Q000379:methods; D002940:Circadian Rhythm; D005260:Female; D005470:Fluorometry; D006719:Homovanillic Acid / Q000652:urine*; D006801:Humans; D007223:Infant; D008297:Male; D010648:Phenylacetates / Q000652:urine*; D012016:Reference Values
D010648:Phenylacetates; D006719:Homovanillic Acid
Dosage de l'acide homovanillique urinaire par chromatographie en phase gazeuse avec colonne capillaire.