title: Nucleosome mass distribution using image averaging.
abstract: Scanning transmission electron microscopy has been used to obtain mass distribution data for unstained, randomly oriented dinucleosomes. These data were then subjected to computer analysis to increase the signal/noise ratio, and define the major sources of image variation within the population. Average images of groups of similar particles have a mass distribution consistent with current models of nucleosome structure, and a resolution approaching 3 nm. The potential value of this method for analyzing structural variants of nucleosomes is discussed.
mesh_terms: D000818:Animals; D002645:Chickens; D004912:Erythrocytes / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009707:Nucleosomes / Q000648:ultrastructure*
chemical_list: D009707:Nucleosomes