Characterization of crystals of protocatechuate 3,4-dioxygenase from Pseudomonas cepacia.
Protocatechuate 3,4-dioxygenase (EC 1.13.11.3) from Pseudomonas cepacia (Mr = 198,000) has been crystallized from potassium phosphate at pH 7.5. Of the two orthorhombic forms that have been characterized, one contains a single molecule of tetrameric protocatechuate dioxygenase, (alpha beta Fe3+)4, per asymmetric unit. The space group and cell dimensions for this form are: P2(1)2(1)2(1) with a = 125.9 A, b = 161.7 A, and c = 85.2 A.
D001386:Azides / Q000138:chemical synthesis / Q000378:metabolism*; D003460:Crystallization; D006150:Guanine Nucleotides; D006153:Guanosine Diphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism; D046911:Macromolecular Substances; D010105:Oxygenases / Q000302:isolation & purification*; D011487:Protein Conformation; D011520:Protocatechuate-3,4-Dioxygenase / Q000302:isolation & purification*; D011549:Pseudomonas / Q000201:enzymology*; D014961:X-Ray Diffraction
D001386:Azides; D006150:Guanine Nucleotides; D046911:Macromolecular Substances; D006153:Guanosine Diphosphate; C043711:8-azidoguanosine-3',5'-diphosphate; D010105:Oxygenases; D011520:Protocatechuate-3,4-Dioxygenase