Preparation and properties of nickel hemoglobin.
Hemoglobin A reconstituted with nickel protoporphyrin IX (NiHbA) has been prepared and characterized. Kinetics of its reaction with p-mercuribenzoate and with haptoglobin, absorption and circular dichroism spectra, and x-ray crystallographic properties have been investigated as probes of its structural conformation. The results suggest that NiHbA exists in a structure that is similar to the deoxy, or T-state of HbA. It is proposed that NiHbA and its derivatives may serve as a useful model for future studies of hemoglobin allosteric changes.
D003460:Crystallization; D006441:Hemoglobin A; D007525:Isoelectric Focusing; D008626:Mercuribenzoates / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010108:Oxyhemoglobins / Q000378:metabolism; D013057:Spectrum Analysis
D008626:Mercuribenzoates; D010108:Oxyhemoglobins; C041281:nickel hemoglobin A; D006441:Hemoglobin A; D010100:Oxygen