Chromatographic method for the isolation of active 40S and 60S subunits from rat liver polyribosomes.
A rapid and simple procedure for isolation of 40S and 60S ribosomal subunits by ion-exchange column chromatography is described. The dissociated ribosomes can be separated and non-ribosomal proteins and low-molecular-weight substances removed. An assessment by physicochemical and functional criteria showed that the ribosomal subunits obtained are active and sufficiently homogeneous.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002852:Chromatography, Ion Exchange / Q000379:methods; D008099:Liver / Q000032:analysis*; D010452:Peptide Biosynthesis; D010455:Peptides; D011072:Poly U / Q000378:metabolism; D011132:Polyribosomes / Q000032:analysis*; D011189:Potassium Chloride / Q000494:pharmacology; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D051381:Rats; D012269:Ribosomal Proteins / Q000302:isolation & purification*
D010455:Peptides; D012346:RNA, Transfer, Amino Acyl; D012269:Ribosomal Proteins; D011072:Poly U; C012001:polyphenylalanine; D011189:Potassium Chloride