Slow migrating proteinase inhibitors in human serum.
Employing a sensitive semi-quantitative electrophoretic-technique on acid-deproteinized serum, we found two previously unrecognized trypsin inhibitors migrating as beta 2- and gamma-globulins, respectively. The two inhibitory bands were also detected in native serum. They were not seen in 3 healthy persons, but were found in patients with uremia, cancer, inflammatory diseases and collagenosis. Immunological investigations showed no cross-reaction with antibodies against seven well-known proteinase inhibitors. The two trypsin inhibitors also inhibited pancreatic elastase.
D000906:Antibodies / Q000032:analysis; D003429:Cross Reactions; D004587:Electrophoresis, Agar Gel; D006801:Humans; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D011480:Protease Inhibitors / Q000097:blood* / Q000276:immunology; D013379:Substrate Specificity; D014361:Trypsin Inhibitors
D000906:Antibodies; D011480:Protease Inhibitors; D014361:Trypsin Inhibitors; D010196:Pancreatic Elastase