Isoelectrofocusing in the study of the Bf system: existence of two common subtypes of the BfF allele.
The inherited polymorphism of the properdin factor B (Bf system) was studied by isoelectrofocusing in polyacrylamide gel (PAGIF, pH range 5.0-8.0), followed by immunofixation. Using the described technique, two different subtypes of F bands are visible (designated as F' and F"). Studies of 13 families and 48 mother-child pairs are not in contradiction to the assumption of a codominant inheritance by two alleles (BfF' and BfF") at a single locus.
D000483:Alleles; D011232:Chemical Precipitation; D002648:Child; D011415:Complement Factor B / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D004792:Enzyme Precursors / Q000235:genetics*; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D011110:Polymorphism, Genetic
D004792:Enzyme Precursors; D011415:Complement Factor B