Binding of methylumbelliferyl mannoside to concanavalin A under high pressure.
The equilibrium binding of 4-methylumbelliferyl alpha-D-mannopyranoside to concanavalin A was measured by changes in fluorescence quenching observed at pressures ranging from 1 to 2000 bar (1974 atmospheres). From the pressure-induced changes in the apparent Ka, we calculated volume changes for the association reaction of -2.5 and -1.7 +/- 1 ml/mol for concanavalin A in its dimeric and tetrameric forms, respectively. This carbohydrate-binding reaction is less pressure-sensitive than other protein-ligand interactions that have been studied under pressure. The volume change observed is comparable to that expected for a reaction involving hydrogen bond formation, in a non-polar environment.
D003208:Concanavalin A / Q000378:metabolism*; D005453:Fluorescence; D006923:Hymecromone / Q000031:analogs & derivatives / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D011312:Pressure; D014468:Umbelliferones / Q000378:metabolism*
D014468:Umbelliferones; D003208:Concanavalin A; C008512:methylumbelliferyl-alpha-D-mannopyranoside; D006923:Hymecromone