[Kinetic heterogeneity of the replication of genetically inactive X chromosome in human cells].
Kinetics of DNA replication in genetically non-active X chromosome was studied in peripheral lymphocytes and skin fibroblasts from four phenotypically normal women and one fetus using BrdU 33258 Hoechst-Giemsa techniques. The localization of the earliest replicated chromosomal segment was shown to be unstable, varying from cell to cell in both lymphocytes and fibroblasts of all persons examined. Several variants of replication sequence in the X chromosome were found in both types of cells. The variants revealed were classified, according to Willard. The statistically significant differences in replication sequence were found between blood lymphocytes and skin fibroblasts in two individuals. The problem of tissue specificity in replication kinetics of the genetically non-active X chromosome is discussed.
D002460:Cell Line; D002478:Cells, Cultured; D002871:Chromosome Banding / Q000379:methods; D004261:DNA Replication; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007621:Karyotyping; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D014960:X Chromosome / Q000378:metabolism*
Geterogenost' kinetiki replikatsii geneticheski neaktivnoĭ X-khromosomy v kletkakh cheloveka.