[Influence of the factors altering the electrophoretic migration of the alpha 2 Gc globulin in the diagram of iso-electric focusing of the Gc type].
In the present study, it is shown that the pattern of the isoelectric focusing with immunofixation changes markedly in the presence of substances such as platelet extract, trypsin, chymotrypsin, vitamin D3 and neuraminidase. It has been recently found that the factor of platelet extract is actin (K. Shinomiya et al., Jpn. J. Legal Med., 36 (1982) 542-549; J. Biochem., 92 (1982) 1163-1171), and Gc globulin is bound by actin (H. Van Baelen et al., J. Biol. Chem., 255 (1980) 2270-2272). The Gc-actin complex appeared as supplementary bands of Gc-globulin near by the anode band of Gc1F-1F or Gc1S-1S. As to the Gc2-2, the supplementary band of Gc-actin complex appeared on the cathode side of the original anode band of Gc1F-1F or Gc1S-1S. Concerning Gc1F-1F (or Gc1S-1S), two supplementary bands of Gc-actin complex appeared on the anode side of the original band. Each product originated from the digestion of Gc-globulins of common phenotypes by trypsin appeared as four characteristic bands on the anode side of the original band. On the other hand, each product obtained by treatment of Gc-globulins of common phenotypes with chymotrypsin appeared as two characteristic bands on the anode side near the anode band of Gc1F-1F. The results obtained in this study give useful information to distinguish the pattern between original and supplementary bands which were altered by the action of factors.
D000199:Actins; D004586:Electrophoresis / Q000379:methods*; D007525:Isoelectric Focusing / Q000379:methods; D011110:Polymorphism, Genetic; D014809:Vitamin D-Binding Protein / Q000302:isolation & purification*
D000199:Actins; D014809:Vitamin D-Binding Protein
Influence des facteurs altérant la migration électrophorétique de l'alpha 2 Gc-globuline sur le diagramme d'iso-électro-focalisation de type Gc.