Transformation of leukotriene A4 methyl ester to leukotriene C4 monomethyl ester by cytosolic rat glutathione transferases.
Six major basic cytosolic glutathione transferases from rat liver catalyzed the conversion of leukotriene A4 methyl ester to the corresponding leukotriene C4 monomethyl ester. Glutathione transferase 4-4, the most active among these enzymes, had a Vmax of 615 nmol X min-1 X mg protein-1 at 30 degrees C in the presence of 5 mM glutathione. It was followed in efficiency by transferase 3-4 which had a Vmax of 160 nmol X min-1 X mg-1 under the same conditions. Transferases 1-1, 1-2, 2-2 and 3-3 had at least 30 times lower Vmax values than transferase 4-4.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology; D005982:Glutathione Transferase / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D017572:Leukotriene A4 / Q000031:analogs & derivatives*; D017997:Leukotriene C4 / Q000031:analogs & derivatives*; D008099:Liver / Q000201:enzymology*; D051381:Rats; D013189:SRS-A / Q000031:analogs & derivatives* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D014316:Tritium
D001095:Arachidonic Acids; D007527:Isoenzymes; D017572:Leukotriene A4; D013189:SRS-A; D014316:Tritium; D017997:Leukotriene C4; C043074:leukotriene C4 monomethyl ester; C043073:leukotriene A methyl ester; D005982:Glutathione Transferase