Specimen preparative methods for electron crystallography of soluble proteins.
Technical factors which influence the choice of specimen preparation method for electron crystallographic study of thin crystals of soluble proteins are discussed. Ice embedding appears to be the most desirable choice of preparation method. However, in terms of the yield of major structural information, we conclude that negative stain remains a useful method for low resolution and glucose embedding for high resolution.
D000818:Animals; D003439:Crotoxin; D003461:Crystallography / Q000379:methods*; D004268:DNA-Binding Proteins; D005615:Freezing; D005947:Glucose; D007141:Immunoglobulin Fc Fragments; D008854:Microscopy, Electron / Q000379:methods*; D011506:Proteins; D013604:T-Phages / Q000648:ultrastructure; D014764:Viral Proteins; D014867:Water
D004268:DNA-Binding Proteins; D007141:Immunoglobulin Fc Fragments; D011506:Proteins; D014764:Viral Proteins; D014867:Water; D003439:Crotoxin; D005947:Glucose