Isolation and characterization of a lectin from edible mushroom, Volvariella volvacea.
A lectin was purified from edible mushroom, Volvariella volvacea by extraction with 5% cold acetic acid in the presence of 0.1% 2-mercaptoethanol, followed by ammonium sulfate fractionation, and DEAE-C-52 and CM-C-52 column chromatographies. The molecular weight was measured to be 26,000, and the lectin consisted of two non-identical subunits as demonstrated by gel filtration and polyacrylamide gel electrophoresis. The lectin does not contain half-cystine, methionine, or histidine. The LD50 of the lectin is 17.5 mg per kg body weight of mice. The lectin has a moderate inhibitory effect on the growth of tumor cells.
D000363:Agaricales / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic; D001001:Anura; D002415:Cats; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens; D004285:Dogs; D004372:Ducks; D004589:Electrophoresis, Disc; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins / Q000302:isolation & purification* / Q000494:pharmacology / Q000633:toxicity; D007928:Lethal Dose 50; D051379:Mice; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D051381:Rats; D012510:Sarcoma 180 / Q000628:therapy; D014357:Trypsin
D000596:Amino Acids; D000972:Antineoplastic Agents, Phytogenic; D037102:Lectins; D010446:Peptide Fragments; D014357:Trypsin