title: DSIP reduces amphetamine-induced hyperthermia in mice.
abstract: The effects of delta sleep-inducing peptide (DSIP) have not been fully determined. Besides sleep-inducing activities, effects on locomotor behavior, stress-reduction, and temperature-regulation have been published. It was reported that DSIP reversed the increase in temperature of rats injected at room temperature with 15 mg d-amphetamine per kg body weight. We examined this effect in mice with 9 different concentrations of DSIP in addition to D-Ala4-DSIP and an analog, DSIP-P, phosphorylated at the serine in position 7. A reduction of the increased temperature was observed in mice but not in rats. This effect was only significant after pretreatment with 0.1 and 150 nmol DSIP/kg, but not the other doses. D-Ala4-DSIP decreased the rise in temperature between 50-150 nmol/kg, but DSIP-P showed no such effect nor was DSIP able to significantly reduce the increase of temperature induced by the larger dose of 30 mg/kg d-amphetamine. A bell-shaped dose-response curve was found for D-Ala4-DSIP; for DSIP two active dose ranges were observed. Thus, complex dose-effect relationships seem to exist for DSIP (and perhaps its analogs) in thermoregulation.
mesh_terms: D000818:Animals; D001833:Body Temperature Regulation / Q000187:drug effects*; D003701:Delta Sleep-Inducing Peptide / Q000031:analogs & derivatives; D003913:Dextroamphetamine / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009842:Oligopeptides / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology
chemical_list: D009842:Oligopeptides; D010455:Peptides; C041971:delta-sleep-inducing peptide amine, Ala(4)-; D003701:Delta Sleep-Inducing Peptide; C033008:delta sleep-inducing peptide phosphate; D003913:Dextroamphetamine