Tumor promoter PMA stimulates the synthesis and secretion of mouse pro-urokinase in MSV-transformed 3T3 cells: this is mediated by an increase in urokinase mRNA content.
In mouse MSV-3T3 cells the synthesis of the urokinase form of plasminogen activator was increased 10-fold after addition of the tumor promoter phorbol-12-myristate-13-acetate (PMA). PMA also stimulated the secretion of the protein into the culture medium, mostly in the form of enzymatically inactive pro-urokinase. When assayed by injecting RNA into Xenopus laevis oocytes, the concentration of functional urokinase mRNA was found to be 6- to 10-fold higher in the PMA-treated cells; a similar increase in urokinase mRNA content was measured by hybridisation with a mouse urokinase cDNA probe. Thus, the induction of plasminogen activator by PMA in MSV-3T3 cells is accounted for by an increased content of urokinase mRNA.
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D004792:Enzyme Precursors / Q000096:biosynthesis; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D051379:Mice; D009865:Oocytes / Q000378:metabolism; D010704:Phorbols / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D014982:Xenopus laevis
D002273:Carcinogens; D004792:Enzyme Precursors; D010704:Phorbols; D012333:RNA, Messenger; D014568:Urokinase-Type Plasminogen Activator; D013755:Tetradecanoylphorbol Acetate