[Effect of protein-calorie restriction during pregnancy in rats, on the activity of glycolytic enzymes in the placenta].
The specific activities and changes of four placental enzymes: pyruvate kinase (PK), glucose-6-phosphate-dehydrogenase (G6PD), 6-phosphogluconic-dehydrogenase (6PGD), and NADP malate dehydrogenase (NADP-MD) occurring during the course of gestation and during maternal restricted food intake, were studied in rats. Enzymes activities are expressed in relation to DNA. With the progress of pregnancy, a significant increase in the activity of all enzymes, except NADP-MD, was observed in both groups. A 50% food restriction during pregnancy markedly decreased cell placental enzymes in different stages of development. PK was lower during early and late pregnancy, but NADP-MD was reduced only in the early developmental stage. The activities of G6PD and 6PGD were significantly lower only in the near-term stage in the malnourished group in comparison with the control group. Our findings suggest that this kind of nutritional insult markedly reduces glycolytic capacity and NADPH2 generation enzymes, a key factor in the placental steroids metabolism.
D000818:Animals; D004734:Energy Metabolism; D005260:Female; D005508:Food Deprivation; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006019:Glycolysis; D008291:Malate Dehydrogenase / Q000378:metabolism; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D010920:Placenta / Q000201:enzymology*; D010922:Placenta Diseases / Q000201:enzymology*; D010927:Placental Insufficiency / Q000201:enzymology* / Q000378:metabolism; D011247:Pregnancy; D011770:Pyruvate Kinase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D008291:Malate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D011770:Pyruvate Kinase
Efecto de la restricción calórico-proteínica durante el embarazo de la rata, en la actividad de algunas enzimas glicolíticas en la placenta.