Isolation and characterization of a novel disialoganglioside from bovine adrenal medulla.
A novel GDlb ganglioside which contains only N-glycolylneuraminic acid was isolated from bovine adrenal medulla by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The concentration of this ganglioside was 0.9 nmol lipid-bound sialic acid per gram fresh tissue, which accounted for 2.7% of the disialoganglioside fraction. The structure was elucidated by sugar analysis, neuraminidase digestion, and permethylation studies. The complete structure of this ganglioside was identified as GDlb (NeuGc)2 or II3 (NeuGc)2-GgOse4Cer.
D000313:Adrenal Medulla / Q000032:analysis*; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000302:isolation & purification*
D005732:Gangliosides; C041884:di-(N-glycolylneuraminic acid)ganglioside GD1b