Husbandry, parasitic and other diseases as factors in the reliability of the enzyme linked immunosorbent assay (ELISA) for detection of trichinellosis in pigs.
Serum samples were obtained from pigs originating from specified pathogen-free farms, large industrialised farms and small conventional farms. All animals proved to be free of Trichinella spiralis by a pooled sample digestion method. Careful meat inspection studies on parasitic infections other than trichinellosis, and other inflammatory reactions were recorded and used for subdivision of the animals in different groups. It was concluded that animal husbandry, parasitic infections, or inflammatory reactions have not influenced the mean extinction values of an ELISA for the detection of T. spiralis antibodies, however, the specified pathogen free animals yielded somewhat lower results.
D000822:Animal Husbandry / Q000379:methods; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D003718:Denmark; D004797:Enzyme-Linked Immunosorbent Assay; D005513:Food Inspection; D008460:Meat; D013552:Swine / Q000469:parasitology; D013553:Swine Diseases / Q000175:diagnosis*; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000175:diagnosis / Q000662:veterinary*
D000906:Antibodies