Differences in surface expression of WGA-binding proteins of cells from a lymphosarcoma and its liver metastases.
The protein and glycoprotein compositions of a subcutaneous lymphosarcoma (1 degree) and its metastatic deposits in the liver (2 degrees) have been investigated in Triton X-100 extracts obtained from tissue, single cells and membrane preparations. No consistent differences in the electrophoretic patterns for 1 degree and 2 degrees tissue or cells were observed for separations visualized with the protein stain Coomassie Blue. Substantial and consistent reductions in the glycoprotein content of extracts from 2 degrees tissue or cells were observed if the separated proteins were treated with the radioiodinated lectin, Wheat Germ Agglutinin (WGA). Densitometric scans of autoradiographs indicated that WGA binding occurred in 4 major areas; the approximate mol. wts of these were 180,000, 102,000, 84,000 and 23,000 daltons. All these components except the 23,000 component were shown to be located in the cell membrane and to be reduced in 2 degrees preparations. Possible sources of host contamination were also investigated, but these did not show WGA binding patterns that were similar to that obtained for the tumour. If 2 degrees tumour was transplanted into the 1 degree site, the resultant growth exhibited a WGA-binding pattern normally shown by a 1 degree tumour growing in this site. Conversely, if 1 degree tumour was transplanted into the liver, the WGA binding of the resultant growth was substantially reduced. The results suggest that local and metastatic tumours do contain cells that express different glycoproteins on their surfaces but that the site of tumour growth is a very important factor in determining this difference in surface expression.
D000818:Animals; D001345:Autoradiography; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis* / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008113:Liver Neoplasms / Q000032:analysis / Q000556:secondary*; D008228:Lymphoma, Non-Hodgkin / Q000032:analysis* / Q000556:secondary; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D008647:Mesocricetus; D009363:Neoplasm Proteins / Q000032:analysis*; D014909:Wheat Germ Agglutinins
D006023:Glycoproteins; D037102:Lectins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D014909:Wheat Germ Agglutinins