title: Regulation and expression of carbamyl phosphate synthetase I mRNA in developing rat liver and Morris hepatoma 5123D.
abstract: A cDNA clone complementary to mRNA encoding the precursor (Mr = 165,000) to the rat liver mitochondrial matrix enzyme carbamyl phosphate synthetase I (Mr = 160,000) was employed to compare relative amounts of the messenger in adult and fetal liver and in Morris hepatoma 5123D and 3924A cells. Northern blot analysis gave a size estimate for the messenger of 6,500-6,700 nucleotides. Carbamyl phosphate synthetase mRNA levels in 15-day-old fetal liver were less than 10% of adult levels; 5123D cells expressed the messenger at levels about 2-fold higher than normal adult liver, but the messenger was undetectable in 3924A cells. Albumin mRNA was also expressed in the former but not in the latter. Maintaining rats for 5 days on a diet containing 60% casein augmented the relative amount of carbamyl phosphate synthetase mRNA by about 2-fold, while a protein-free diet resulted in reduced levels of the mRNA (about 50% compared to animals on a normal diet). Finally, the pattern of hybridization of carbamyl phosphate synthetase cDNA to HindIII-digested genomic DNA showed no differences between normal liver and its corresponding hepatoma; however, a HindIII site polymorphism was observed between Buffalo and ACI rats.
mesh_terms: D000818:Animals; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D005333:Fetus; D005796:Genes; D008025:Ligases / Q000235:genetics*; D008099:Liver / Q000201:enzymology* / Q000254:growth & development; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008297:Male; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011912:Rats, Inbred ACI; D011915:Rats, Inbred BUF; D011919:Rats, Inbred Strains
chemical_list: D012333:RNA, Messenger; D004247:DNA; D008025:Ligases; D002222:Carbamoyl-Phosphate Synthase (Ammonia)