The preparation of freeze-thaw density gradients with homogeneous solute concentrations.
A simple and effective method is described for preparing freeze-thaw density gradients which is free of the problems of uneven solute distribution. It consists of blending aqueous freeze-thaw gradients with hypertonic diluent. The routine application of this system for separations of murine lymphosarcoma cell populations is described.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D005615:Freezing; D007552:Isotonic Solutions; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology; D051379:Mice; D008808:Mice, Inbred CBA
D007552:Isotonic Solutions