title: [Lipoamide dehydrogenase, citrate synthase and beta-hydroxyacyl-CoA-dehydrogenase in skeletal muscles. IV. The effect of storage of bovine and swine muscles at 2 degrees C on their activity and subcellular distribution].
abstract: Postmortem storage of bovine and porcine semimembranosus muscle under refrigeration (+2 degrees C) for 14 days did not result in a significant decrease of the total activity of the mitochondrial enzymes citrate synthase and beta-hydroxyacyl-CoA-dehydrogenase, but caused a loss of the activity of lipoamide dehydrogenase. During storage of the muscle tissue there was no detectable release of the three enzymes from the mitochondria into the sarcoplasmic fluid. Therefore, storage of muscle under the conditions used seems not to result in any remarkable damage of the inner membrane of the mitochondrion.
mesh_terms: D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002950:Citrate (si)-Synthase / Q000378:metabolism*; D003080:Cold Temperature; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism*; D066298:In Vitro Techniques; D009132:Muscles / Q000201:enzymology*; D007652:Oxo-Acid-Lyases / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D013045:Species Specificity; D013347:Subcellular Fractions / Q000201:enzymology; D013552:Swine; D013997:Time Factors
chemical_list: D011506:Proteins; D015094:3-Hydroxyacyl CoA Dehydrogenases; D008058:Dihydrolipoamide Dehydrogenase; D002950:Citrate (si)-Synthase; D007652:Oxo-Acid-Lyases
vernacular_title: Lipoamiddehydrogenase, Citratsynthase und beta-hydroxyacyl-CoA-dehydrogenase des Skelettmuskels. IV. Einfluss der Lagerung von Rinder- und Schweinemuskel bei +2 grade C auf Aktivität und subcelluläre Verteilung.