DTIC and CPZ cytotoxicities on established human melanocyte cell lines.
We report the effects of one cytostatic and one anti-emetic, DTIC and chlorpromazine (CPZ), on eight established human melanocyte cell lines using 3 methods of evaluation. Considerable differences in chemo-sensitivity to DTIC were discovered. Both inhibition, stimulation and no effect were seen. CPZ always showed cytotoxic effect in vitro. DTIC combined with CPZ had an antagonistic effect except for one cell line which was synergistic. From the chemosensitivity obtained with a primary tumor, it was impossible to predict that of its metastasis. Two similar metabolic effects in vitro were not necessarily related to identical therapeutic sensitivity in vivo. At least 3 significant results must be obtained in vitro to predict a therapeutic schedule.
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002746:Chlorpromazine / Q000494:pharmacology*; D003606:Dacarbazine / Q000494:pharmacology*; D004351:Drug Resistance; D006801:Humans; D008544:Melanocytes / Q000187:drug effects*; D008545:Melanoma / Q000473:pathology*
D003606:Dacarbazine; D002746:Chlorpromazine