Carrier and adjuvant properties of liposome-borne tumor-specific antigens.
The purpose of this study was to examine (1) the association of tumor extract proteins with phospholipid vesicles of varying physiochemical properties, and (2) the adjuvant and carrier properties of liposome-borne tumor antigens in the in vivo induction of an antitumor immune response. Cell surface antigens of the 3-methylcholanthrene-induced fibrosarcoma of C3H/HeJ mice, MCA-F, were extracted using 2.5% 1-butanol. Crude and electrofocused antigen preparations capable of eliciting a protective antitumor immune response were used to prepare liposome vaccines. The incorporation of extract proteins into liposomes formed by butanol dialysis (BVD) was three- to five-fold greater than the encapsulation of protein into the aqueous compartment of multilamellar vesicles (MLV). The electrochemical properties of the BDV had a significant effect on the induction of an antitumor response: Antigens borne on negatively charged, but not uncharged, liposomes were effective in protecting hosts against supralethal tumor challenge, and displayed a specific activity 20- to 50-fold greater than soluble antigen. Antigens carried by MLV were not effective in generating an immunoprotective response. The lipophilic characteristics of butanol-extracted antigens allowed (1) the passive adsorption of immunoprotective tumor antigen onto the surface of preformed vesicles, and (2) adsorption of MCA-F antigen onto the surface of an antigenically distinct tumor MCA-D. In the latter experiment, adsorption of MCA-F-specific antigen onto MCA-D cells resulted in a change in the membrane antigen phenotype as measured by indirect immunofluorescence. Although butanol released a lipophilic moiety from cells which spontaneously reassociated with phospholipid bilayers, no evidence for a lipoidal antigen was obtained when tumor-derived lipids were used as immunogens. This study demonstrates that butanol-extracted tumor antigen is lipophilic without being a lipid, and that negatively charged liposomes can be effective as carriers and adjuvants for tumor antigens in the induction of an antitumor immune response.
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000327:Adsorption; D000818:Animals; D000951:Antigens, Neoplasm / Q000008:administration & dosage* / Q000276:immunology / Q000302:isolation & purification; D002352:Carrier Proteins / Q000008:administration & dosage* / Q000276:immunology; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D005354:Fibrosarcoma / Q000276:immunology* / Q000378:metabolism; D008081:Liposomes / Q000276:immunology*; D008563:Membrane Lipids / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H
D000276:Adjuvants, Immunologic; D000951:Antigens, Neoplasm; D002352:Carrier Proteins; D008081:Liposomes; D008563:Membrane Lipids