Sarcoid granulomas metabolize 25-hydroxyvitamin D3 in vitro.
Sarcoid granulomas metabolized 25-hydroxyvitamin D3 to two unidentified metabolites during in vitro incubation. A two-step high pressure liquid chromatography system revealed two unique elution positions of these sarcoid-derived metabolites that exactly comigrated with the elution positions of 5(Z)-19-nor-10-oxo-25(OH)D3 and 5(E)-19-nor-10-oxo-25(OH)D3, respectively. These unique metabolites did not bind specifically to a protein receptor for 1,25(OH)2D3.
D001706:Biopsy; D002112:Calcifediol / Q000378:metabolism*; D002117:Calcitriol / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006099:Granuloma / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008198:Lymph Nodes / Q000378:metabolism; D008206:Lymphatic Diseases / Q000378:metabolism*; D012507:Sarcoidosis / Q000378:metabolism*
D002117:Calcitriol; D002112:Calcifediol