Prednisolone decreases exercise-induced acid hydrolase response in mouse skeletal muscle.
Male NMRI-mice were subjected to exhaustive treadmill exercise. 3 and 6 days after the exertion, quadriceps femoris muscles were examined histologically and analyzed for acid hydrolases in order to follow the degree and progress of injuries. Prednisolone (PRED), an anti-inflammatory corticosteroid, was given to some of the animals in order to modify the exercise response. The PRED administration began 14 h before exercise and continued until the end of the experiment (6 days). The doses were 25 and 50 mg . kg-1 i.p. twice a day. The activities of both arylsulphatase and beta-glucuronidase increased significantly in the exercise control group after 3 and 6 days. The increase in activity correlated with fibre necrosis and an abundant infiltration of inflammatory cells, and was greatest after 3 days. After 6 days the inflammatory response decreased and regenerating muscle fibres were seen. PRED decreased the exercise-induced acid hydrolase response. The decrease was most prominent after 3 days with PRED 50 mg . kg-1 . day-1. PRED also diminished degeneration and inflammation. The results suggest that the decrease in acid hydrolase activities was due to a lesser infiltration of inflammatory cells to the injured area.
D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism*; D003864:Depression, Chemical; D005080:Exercise Test; D005947:Glucose / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009132:Muscles / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D009135:Muscular Diseases / Q000209:etiology; D009220:Myositis / Q000201:enzymology / Q000209:etiology; D005082:Physical Exertion; D011239:Prednisolone / Q000494:pharmacology*; D012038:Regeneration; D013429:Sulfatases / Q000378:metabolism*
D011239:Prednisolone; D013429:Sulfatases; D001192:Arylsulfatases; D005966:Glucuronidase; D005947:Glucose