[Determination of the binding of 3,5-bis(acetamido)-2,4,6-triiodobenzoic acid and some of its salts to human serum albumin using the ultrafiltration flat chamber method].
Using the ultrafiltration flat chamber method the binding of N-methylglucamine, meglumine amido trizoate, lysine amido trizoate, diatrizoic acid and L-lysine to human serum albumin (HSA) was investigated. It was demonstrated that the affinity to HSA decreases in the above mentioned order. Binding was characterised by determination of K1 (total binding constant), k+ (apparent binding constant), n (number of binding sites per molecule of albumin) and delta F degrees (free reaction energy).
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003973:Diatrizoate / Q000097:blood*; D006801:Humans; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D014462:Ultrafiltration
D012709:Serum Albumin; D003973:Diatrizoate
Bestimmung der Bindung von 3,5-Bis-(acetamido)-2,4,6-trijodbenzoesäure sowie einigen ihrer Salze an Humanserumalbumin mit Hilfe der Ultrafiltrationsflachkammer-Methode.