title: [Availability of DNP-modified 5'- and 3'-ends of the poly(U) fragment bound to the 30S ribosomal subunit for interaction with antibodies].
abstract: Modification of the 5'- or 3'-terminal nucleotide residues of poly(U) by dinitrophenyl haptens was carried out. The accessibility of the modified ends of short poly(U) (20-70 nucleotide residues) bound to the 30S subunit of E. coli ribosomes for the interaction with antibodies that are specific against the dinitrophenyl group, was investigated. Some peculiarities of the structural organization of the "entry" (3'-end) and "exit" (5'-end) sites of the template polynucleotide on the 30S subunit and the possibility of the template polynucleotide folding on the ribosome are discussed.
mesh_terms: D000906:Antibodies / Q000276:immunology / Q000378:metabolism*; D001482:Base Composition; D001666:Binding Sites, Antibody; D004136:Dinitrobenzenes / Q000276:immunology / Q000494:pharmacology*; D006241:Haptens / Q000276:immunology / Q000494:pharmacology; D006868:Hydrolysis; D009578:Nitrobenzenes / Q000494:pharmacology*; D011072:Poly U / Q000276:immunology / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012270:Ribosomes / Q000276:immunology / Q000378:metabolism*; D013698:Templates, Genetic
chemical_list: D000906:Antibodies; D004136:Dinitrobenzenes; D006241:Haptens; D009578:Nitrobenzenes; D012346:RNA, Transfer, Amino Acyl; D011072:Poly U
vernacular_title: Dostupnost' DNF-modifitsirovannykh 5'- i 3'-kontsov fragmenta poli(U), sviazannogo s 30 S-subchastitseĭ ribosomy, dlia vzaimodeĭstviia s antitelami.