Components of the kallikrein-kinin system in rat urine.
Using a direct radioimmunoassay and a kininogenase assay, we determined that 68% of rat urinary kallikrein was enzymatically active while 32% was in an inactive form which was activated by trypsin. Inorganic cations, at concentrations found in rat urine, were inhibitory in an amidase assay but appeared to potentiate kininogenase activity of pure rat urinary kallikrein. In random urines, kinin concentration was 4.2 +/- 0.7 ng/ml. Trypsinization of the urines generated 52.9 +/- 25.8 ng kinin/ml, indicating that kininogen was present. The rate of kinin formation in vivo may depend on the availability of kininogen and the concentration of inorganic cations in urine, as well as on other well-recognized factors, such as the kallikrein activity of the urine.
D000818:Animals; D007610:Kallikreins / Q000652:urine*; D007704:Kininogens / Q000652:urine; D007705:Kinins / Q000652:urine*; D011863:Radioimmunoassay; D051381:Rats; D014357:Trypsin / Q000378:metabolism
D007704:Kininogens; D007705:Kinins; D007610:Kallikreins; D014357:Trypsin