Validation of cytochemical section bioassay for thyroid-stimulating immunoglobulins in treated and untreated Graves' disease.
The cytochemical section-bioassay of thyroid stimulating activity is described. Plasma of thyrotoxic patients as well as those on block-replace treatment with carbimazole and thyroid hormones was used. Linear parallel responses were obtained over the range 1.5 X 10(-7) to 1.5 X 10(-5) mU/1 MRC LATS-B standard. The index of precision was 0.19 +/- 0.028. The fiducial limits (p=0.95) of a sample tested on ten separate occasions were 52-192%. Specificity was investigated using time course studies, and the effect of anti-TSH or anti-IgG antisera. No effect of methimazole or a variety of other drugs was detected. The assay is accordingly validated for measurement of TSI in patients both untreated and on block-replace therapy.
D000328:Adult; D000626:Aminopeptidases / Q000032:analysis; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002231:Carbimazole / Q000627:therapeutic use; D005260:Female; D006111:Graves Disease / Q000188:drug therapy / Q000276:immunology*; D006168:Guinea Pigs; D006651:Histocytochemistry; D006801:Humans; D008135:Long-Acting Thyroid Stimulator / Q000097:blood*; D008297:Male; D013961:Thyroid Gland / Q000201:enzymology; D013963:Thyroid Hormones / Q000627:therapeutic use
D013963:Thyroid Hormones; D002231:Carbimazole; D008135:Long-Acting Thyroid Stimulator; D000626:Aminopeptidases