Positive identification of a measles virus cDNA clone encoding a region of the phosphoprotein.
A measles virus-specific cDNA clone, C1-G, that was previously assigned as a hemagglutinin-specific clone has been reassigned as a phosphoprotein-specific clone. The nucleotide sequence of C1-G was used to deduce the amino acid sequence. A synthetic peptide was constructed from a portion of the deduced sequence, and antisera were prepared. The antibodies directed against this synthetic peptide specifically precipitated the phosphoprotein of measles virus and not the hemagglutinin.
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D005796:Genes; D005814:Genes, Viral; D007668:Kidney; D008459:Measles virus / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D014176:Protein Biosynthesis; D014764:Viral Proteins / Q000235:genetics*
D010750:Phosphoproteins; D014764:Viral Proteins; D004247:DNA