Experimental pulmonary emphysema induced in the rat by intratracheally administered elastase: morphogenesis.
Emphysema was produced experimentally in rats by administration of porcine pancreatic elastase at dose levels of 75, 100, 125, and 150 units elastase activity/100 g body wt. All doses studied were equally effective in producing emphysema. Dose levels of over 75 units activity/100 g offered no advantage in terms of degree or severity of emphysema produced; however, such doses exacerbated hemorrhage and edema, resulting in relatively high post-instillation mortality. Lesion morphogenesis at all doses tested paralleled those described in this and in other species by other investigators. The 75-unit activity produced a quantifiable degree of emphysema which was relatively constant among animals. Results suggest that because of group differences in susceptibility to elastase (or variations in batches of elastase), dose-range studies should be performed on each new group of animals.
D000818:Animals; D008168:Lung / Q000187:drug effects* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010196:Pancreatic Elastase / Q000633:toxicity*; D011656:Pulmonary Emphysema / Q000139:chemically induced* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains
D010196:Pancreatic Elastase