Purification and characterization of the ninth component of rabbit complement.
A method of purification of C9 from rabbit serum is described. The three-step procedure, consisting of anion exchange chromatography, gel-filtration and isoelectric focusing yielded a homogeneous, single band protein as judged by SDS-PAGE. With regard to its physicochemical properties, rabbit C9 resembled C9 purified from human or guinea-pig serum.
D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003186:Complement C9 / Q000302:isolation & purification*; D006168:Guinea Pigs; D006801:Humans; D007525:Isoelectric Focusing; D008970:Molecular Weight; D011817:Rabbits; D013045:Species Specificity
D003186:Complement C9