Rapid increase of mitochondrial uncoupling protein and its mRNA in stimulated brown adipose tissue. Use of a cDNA probe.
The increase in mitochondrial uncoupling protein in brown adipose tissue during acute stimulation by exposure of animals to cold was examined. Uncoupling protein level increased during the first hours of tissue stimulation. Use of a cDNA probe shows that synthesis of uncoupling protein mRNA was quickly stimulated. Animals treated with propranolol exhibited neither increase in uncoupling protein mRNA nor increase in the protein itself.
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins; D003080:Cold Temperature; D004247:DNA; D006153:Guanosine Diphosphate / Q000378:metabolism; D007473:Ion Channels; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D024101:Mitochondrial Proteins; D009693:Nucleic Acid Hybridization; D011433:Propranolol / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D000071256:Uncoupling Protein 1; D006153:Guanosine Diphosphate; D004247:DNA; D011433:Propranolol