Isolation and primary structure of human PHI (peptide HI).
The isolation of the human form of PHI (peptide HI) is described. The peptide was purified from human colonic extracts by using a chemical method for the detection of its C-terminal amidated structure. Human PHI consists of 27 amino acid residues and the complete amino acid sequence is: His-Ala-Asp-Gly-Val-Phe-Thr-Ser-Asp-Phe-Ser-Lys-Leu-Leu-Gly-Gln-Leu-Ser- Ala-Lys-Lys-Tyr-Leu-Glu-Ser-Leu-Met-NH2. The differences between the structures of porcine and human PHI are at position 12 (Arg/Lys replacement) and at position 27 (Ile/Met).
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000032:analysis*; D003110:Colonic Neoplasms / Q000032:analysis; D005590:Fractional Precipitation; D006801:Humans; D010451:Peptide PHI; D010455:Peptides / Q000302:isolation & purification*; D013552:Swine
D010451:Peptide PHI; D010455:Peptides