Nucleosome mass distribution using image averaging.
Scanning transmission electron microscopy has been used to obtain mass distribution data for unstained, randomly oriented dinucleosomes. These data were then subjected to computer analysis to increase the signal/noise ratio, and define the major sources of image variation within the population. Average images of groups of similar particles have a mass distribution consistent with current models of nucleosome structure, and a resolution approaching 3 nm. The potential value of this method for analyzing structural variants of nucleosomes is discussed.
D000818:Animals; D002645:Chickens; D004912:Erythrocytes / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009707:Nucleosomes / Q000648:ultrastructure*
D009707:Nucleosomes