[A method for determination of prostaglandins in human seminal fluid].
Human seminal fluid has been shown to contain mainly eleven different prostaglandins. Methods for quantitative determination of prostaglandin E and 19-OH prostaglandin E are described. The methods include extraction with acetone and alkali treatment. After alkali treatment prostaglandin E was transformed to prostaglandin B, and 19-OH prostaglandin E to 19-OH prostaglandin B. Prostaglandins were separated by using Lipidex column chromatography and silicic acid column chromatography. Prostaglandin B was quantitated by gas chromatography using C24 fatty acid methyl ester as internal standard. 19-OH prostaglandin B was quantitated by measuring ultraviolet photoabsorbance at 278 nm.
D000527:Alprostadil / Q000031:analogs & derivatives*; D002845:Chromatography; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D008722:Methods; D011458:Prostaglandins E / Q000032:analysis* / Q000302:isolation & purification; D012661:Semen / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D011458:Prostaglandins E; C025128:19-hydroxyprostaglandin E1; D000527:Alprostadil