Pulsatile secretion of gonadotrophins, ovarian steroids and ovarian oxytocin during prostaglandin-induced regression of the corpus luteum in the cow.
A luteolytic dose (500 micrograms) of cloprostenol was given on Day 12 of the oestrous cycle to 5 heifers. Blood samples were collected simultaneously from the caudal vena cava and jugular vein at 5-20-min intervals from -6 to 0 (control period), 0 to 12 and 24 to 36 h after PG injection. Pulses of LH were secreted concomitantly with pulses of FSH during all sampling periods. However, during the control period separate FSH pulses were detected resulting in a shorter (P less than 0.01) interpulse interval for FSH than LH (93 versus 248 min). LH and FSH pulse frequencies increased (P less than 0.01) beginning 1-3 h after PG to interpulse intervals of 59 and 63 min, respectively, and continued to be maintained 24-36 h after PG. Concomitantly there was a 2-3-fold increase (P less than 0.01) in basal concentrations and pulse amplitude for LH (but not FSH). FSH basal concentrations and pulse amplitudes decreased (P less than 0.05) in 3 heifers 24-36 h after PG. Pulsatile secretion of oestradiol was observed at frequencies similar to LH during the periods 4-12 h (3 heifers) and 24-36 h (2 heifers) after PG, respectively, resulting in higher (P less than 0.05) mean oestradiol concentrations. Progesterone concentrations in the vena cava increased (P less than 0.01) 5-10 min after PG but decreased (P less than 0.01) 67% by 20 min after PG. This decrease was followed by a rise (P less than 0.05) beginning 2-3 h after PG and lasting for an average of 3.3 h.(ABSTRACT TRUNCATED AT 400 WORDS)
D000818:Animals; D002417:Cattle; D003008:Cloprostenol / Q000494:pharmacology; D003338:Corpus Luteum / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D004971:Estrus; D005260:Female; D006065:Gonadotropins, Pituitary / Q000378:metabolism*; D003341:Luteolysis / Q000187:drug effects; D008686:Metestrus; D010053:Ovary / Q000378:metabolism; D010121:Oxytocin / Q000378:metabolism*; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism*; D012636:Secretory Rate
D006065:Gonadotropins, Pituitary; D003008:Cloprostenol; D011374:Progesterone; D004958:Estradiol; D010121:Oxytocin