Distribution of intermediate filaments and actin microfilaments in parotid autoimmune sialoadenitis of Sjögren syndrome.
In order to study the importance of myoepithelial cells in the formation of parotid epi-myoepithelial islands of the autoimmune sialoadenitis in Sjögren's syndrome, the distribution of cytoskeletal elements has been investigated by electron and immunofluorescence microscopy. Antibodies to intermediate filament proteins--prekeratins, desmin and vimentin--were used as well as a 'DNase I/anti-DNase I' immunohistochemical procedure to detect actin microfilaments for characterization of myoepithelial cells compared to the other types of parotid epithelial cells. Two types of prekeratin antibodies were raised. One of these reacted preferentially with myoepithelial cells. The same type of antiserum also showed a bright fluorescence in cells located peripherally in the epi-myoepithelial islands. These island cells, however, exhibited only a very low actin microfilament content and were thus not ordinary myoepithelial cells. The other type of prekeratin antibodies reacted with all types of parotid epithelia, except acinar secretory epithelium. They also stained all island epithelial cells, but the reactivity was less in cell regions with pronounced lymphocyte infiltration. The heterogeneity between the island epithelial cells, immunohistochemically as well as ultrastructurally, indicates different stages of island formation. Interepithelial lymphocytes showed less vimentin and actin filament content in comparison with lymphoid cells outside the islands. Conclusively, typical epi-myoepithelial islands are composed of metaplastic epithelial cells of intercalated duct origin and invaded lymphoid cells, and ordinary myoepithelial cells are lacking.
D000199:Actins; D000328:Adult; D000368:Aged; D003599:Cytoskeleton / Q000473:pathology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007381:Intermediate Filament Proteins / Q000276:immunology; D008297:Male; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D012466:Salivary Gland Diseases / Q000473:pathology*; D012793:Sialadenitis / Q000276:immunology / Q000473:pathology*; D012859:Sjogren's Syndrome / Q000276:immunology / Q000473:pathology*
D000199:Actins; D007381:Intermediate Filament Proteins