Synthesis of phosphorothioate-containing DNA fragments by a modified hydroxybenzotriazole phosphotriester approach.
The phosphorothioylating agent which was obtained by treating 2,5-dichlorophenyl phosphorodichloridothioate with 1-hydroxy-6-nitrobenzotriazole proved to be very effective for the synthesis in solution and on a solid support of phosphorothioate-containing DNA fragments.
D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D063086:Organothiophosphates; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D014230:Triazoles
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D063086:Organothiophosphates; D014230:Triazoles; C043838:1-hydroxy-6-nitrobenzotriazole; C043839:2,5-dichlorophenyl phosphorodichloridothioate; D004247:DNA