title: In vitro cultivation of Dipetalonema viteae third-stage larvae: evaluation of culture media, serum, and other supplements.
abstract: Third-stage larvae of Dipetalonema viteae obtained from the tick vector developed to the fourth stage in several cell-free culture systems. Survival and development of larvae in a number of commercially available cell culture media, supplemented with serum and other defined and undefined components, were compared. All cultures were gassed with 5% carbon dioxide in nitrogen. Best survival, growth and development were obtained in stationary cultures containing 1:1 (v/v) mixtures of NCTC 135, either RPMI 1640 or Iscove's Modified Dulbecco's Medium, and a supplement of 20% non-heat-inactivated fetal bovine serum. The importance of the medium composition and physical environment of the culture system, for the survival, growth and development of D. viteae was demonstrated.
mesh_terms: D000818:Animals; D001769:Blood; D002417:Cattle; D003470:Culture Media; D004153:Dipetalonema / Q000166:cytology / Q000254:growth & development*; D005260:Female; D066298:In Vitro Techniques; D007303:Insect Vectors; D007814:Larva / Q000502:physiology; D013987:Ticks
chemical_list: D003470:Culture Media