Effect of bile acid analogs on 7 alpha-dehydroxylase activity in Eubacterium sp. V.P.I. 12708.
7 beta-Methyl-chenodeoxycholic acid (7-MeCDC, 3 alpha, 7 alpha-dihydroxy-7 beta-methyl-5 beta-cholan-24-oic acid), 7 alpha-methyl-ursodeoxycholic acid (7-MeUDC, 3 alpha, 7 beta-dihydroxy-7 alpha-methyl-5 beta-cholan-24-oic acid), 7 xi-methyl-lithocholic acid (7-MeLC, 3 alpha-hydroxy-7 xi-methyl-5 beta-cholan-24-oic acid) and ursodeoxycholylsarcosine (UDCS) were tested as inhibitors of bacterial bile acid 7 alpha-dehydroxylase activity. At a concentration of 50 microM, 7-MeCDC and 7-MeUDC inhibited enzyme activity by 66% and 12%, respectively. 7 alpha-Dehydroxylase activity was not inhibited in the presence of 7-MeLC and UDCS. None of the four bile acid analogs tested inhibited the growth of Eubacterium sp. V.P.I. 12708 at concentrations up to 100 microM.
D000438:Alcohols / Q000494:pharmacology; D001647:Bile Acids and Salts / Q000494:pharmacology*; D002635:Chenodeoxycholic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D005051:Eubacterium / Q000201:enzymology*; D006913:Hydroxysteroid Dehydrogenases; D007700:Kinetics; D008095:Lithocholic Acid / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000494:pharmacology; D010088:Oxidoreductases; D013250:Steroid Hydroxylases / Q000378:metabolism*; D014580:Ursodeoxycholic Acid / Q000031:analogs & derivatives / Q000494:pharmacology
D000438:Alcohols; D001647:Bile Acids and Salts; D002635:Chenodeoxycholic Acid; D008095:Lithocholic Acid; D014580:Ursodeoxycholic Acid; C043346:sarcoursodeoxycholic acid; C041636:3,7-dihydroxy-7-methylcholanoic acid; C041637:3-hydroxy-7-methylcholanoic acid; D010088:Oxidoreductases; D006913:Hydroxysteroid Dehydrogenases; C025376:7 alpha-hydroxysteroid dehydrogenase; D013250:Steroid Hydroxylases