Light microscopic autoradiographic localization of [3H]pirenzepine and [3H](-)quinuclidinyl benzilate binding in human stellate ganglia.
We have utilized the LKB Ultrofilm method of autoradiography to anatomically localize putative M1 and M2 muscarinic receptor subtypes in human stellate ganglia. Ten micron sections were labeled in vitro with either 1 nM of the classical antagonist [3H](-)quinuclidinyl benzilate ([3H](-)QNB) or 20 nM of the non-classical antagonist [3H]pirenzepine ([3H]PZ), using 1 microM atropine sulfate to define non-specific binding for both ligands. Our results indicate that [3H](-)QNB and [3H]PZ binding sites are distributed within the principal ganglion cells and nerve bundles.
D001345:Autoradiography; D001570:Benzodiazepinones / Q000378:metabolism*; D006801:Humans; D010276:Parasympatholytics / Q000378:metabolism*; D010890:Pirenzepine; D011812:Quinuclidines / Q000378:metabolism*; D011813:Quinuclidinyl Benzilate / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000378:metabolism*; D013233:Stellate Ganglion / Q000378:metabolism*; D014316:Tritium
D001570:Benzodiazepinones; D010276:Parasympatholytics; D011812:Quinuclidines; D011976:Receptors, Muscarinic; C028484:quinuclidinyl benzilate receptor; D014316:Tritium; D010890:Pirenzepine; D011813:Quinuclidinyl Benzilate