Are lymphocyte subset determinations affected by storage conditions?
The effect of overnight storage, either at room temperature or at 4 degrees C, on total T cells, helper cells, suppressor cells, and HLA-DR-positive cells was determined by flow cytometry and fluoresceinated monoclonal antibodies on peripheral whole blood samples. No differences were noted in any of the subsets regardless of storage conditions when samples were analyzed within 24 hr.
D000328:Adult; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D001793:Blood Preservation; D006801:Humans; D007958:Leukocyte Count; D008214:Lymphocytes / Q000145:classification* / Q000276:immunology; D013696:Temperature
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface