Molecular cloning of hepatic 5-aminolevulinate synthase.
Hepatic 5-aminolevulinate synthase was induced in chick embryos by administration of the porphyrinogenic drugs 2-allyl-2-isopropylacetamide and 3,5-diethoxycarbonyl-1,4-dihydrocollidine. A cDNA library was constructed from drug-induced liver mRNA and clones containing sequences coding for 5-aminolevulinate synthase were identified by hybrid-selected translation. The identity of these clones was confirmed by comparing DNA sequence data with the amino acid sequence of peptides from purified 5-aminolevulinate synthase. From Northern blot analysis the size of the mRNA for 5-aminolevulinate synthase was estimated to be 2800 base pairs, approximately 600 base pairs more than that required to code for the primary translation product of relative molecular mass 74000.
D000624:5-Aminolevulinate Synthetase / Q000096:biosynthesis / Q000235:genetics*; D000502:Allylisopropylacetamide / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D003997:Dicarbethoxydihydrocollidine / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D008099:Liver / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000302:isolation & purification
D012333:RNA, Messenger; D000502:Allylisopropylacetamide; D003997:Dicarbethoxydihydrocollidine; D004247:DNA; D000624:5-Aminolevulinate Synthetase