Injection of partially purified estrogen receptor protein from Xenopus liver nuclei into oocytes activates the silent vitellogenin locus.
Injection of extracts from Xenopus liver nuclei that are enriched 2000 times in estradiol receptor into Xenopus oocytes induces transcription of the silent vitellogenin locus, which is activated in liver by estradiol, but not of the albumin locus, which is active in liver but suppressed by high levels of estradiol. Transcription initiates within the 5'-end region of the gene we have studied and probably continues into the 3' third. The activation seems to be very efficient, but most of the primary transcripts are probably rapidly and inaccurately processed. New proteins are also made and secreted by the oocytes.
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000502:physiology*; D004958:Estradiol / Q000502:physiology; D005260:Female; D005796:Genes; D008074:Lipoproteins / Q000235:genetics*; D008099:Liver / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D011959:Receptors, Estradiol; D012709:Serum Albumin / Q000235:genetics; D014158:Transcription, Genetic; D014819:Vitellogenins / Q000235:genetics* / Q000302:isolation & purification; D014981:Xenopus
D008074:Lipoproteins; D012333:RNA, Messenger; D011959:Receptors, Estradiol; D012709:Serum Albumin; D014819:Vitellogenins; D004958:Estradiol