Cyclosporin A on human corneal endothelium.
Human corneal endothelial cells grown in culture medium were exposed to different concentrations of cyclosporin A in vitro. After 7 days, concentrations of the drug less than 1,000 ng/ml of medium were found nontoxic to the endothelial layer, while greater concentrations killed the cells in 1 hour to 48 hours, depending upon the concentration of the drug. Degenerative cytoplasmic vacuoles were the first sign of toxicity from the drug, leading in higher concentrations to eventual disintegration.
D003315:Cornea / Q000187:drug effects*; D003524:Cyclosporins / Q000506:poisoning*; D004727:Endothelium / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques
D003524:Cyclosporins