Histochemical detection of thiosulphate sulphurtransferase (rhodanese) activity.
A histochemical method is described by which the activity of thiosulphate sulphurtransferase (E.C. 2.8.1.1., rhodanese) can be detected. The method gives an easy and quick determination of enzyme activity. The basis of the detection is the reducing effect of sulphite (SO 2/3) ions resulting from the enzyme reaction. Nitro-blue tetrazolium chloride can be used as an indicator and N-methylphenazonium-methosulphate as an accelerator for increasing electron transport. In mammals rhodanese is localized in the mitochondria.
D000818:Animals; D006801:Humans; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008773:Methylphenazonium Methosulfate; D008928:Mitochondria / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D009580:Nitroblue Tetrazolium; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical; D013466:Sulfurtransferases / Q000032:analysis*; D013583:Synovial Membrane / Q000201:enzymology; D013884:Thiosulfate Sulfurtransferase / Q000032:analysis*
D009580:Nitroblue Tetrazolium; D008773:Methylphenazonium Methosulfate; D013466:Sulfurtransferases; D013884:Thiosulfate Sulfurtransferase