Lampbrush loop-specific protein of Drosophila hydei.
By immunofluorescence techniques and protein blotting experiments we have shown that an antiserum specifically reacts with a Mr 80,000 protein (the "Ps protein") in the lampbrush loop " pseudonucleolus " in spermatocyte nuclei of Drosophila hydei. Comparative studies of X/Y and X/0 testes indicate that the gene encoding the Ps protein is not located on the Y chromosome but on an autosome or the X chromosome. The Ps protein is tissue specific. It is likely to be a rather conserved protein since the antigenic determinant recognized by the antiserum could be detected in the spermatocyte nuclei of a number of other Drosophila species. For those species with prominent Y chromosomal lampbrush loops, it could be shown that the cross-reaction is, as in D. hydei, associated with a specific Y chromosomal loop.
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics* / Q000276:immunology; D002874:Chromosome Mapping; D004330:Drosophila / Q000235:genetics*; D005796:Genes; D008297:Male; D008970:Molecular Weight; D013045:Species Specificity; D013090:Spermatocytes / Q000648:ultrastructure; D014998:Y Chromosome / Q000648:ultrastructure*
D002868:Chromosomal Proteins, Non-Histone