Direct observation of delta-crystallin accumulation by laser light-scattering spectroscopy in the chicken embryo lens.
By using the technique of laser light-scattering spectroscopy, direct observation has been made on the intracellular accumulation of a crystallin protein within the cells of chicken embryo lens during the process of development. Appearance of delta-crystallin has been detected as early as day 4, and its concentration reaches a plateau at day 19. The measurements constitute a noninvasive determination of accumulation of protein molecules that specifically characterize the process of cell differentiation.
D000818:Animals; D002642:Chick Embryo; D003459:Crystallins / Q000032:analysis*; D007834:Lasers; D007908:Lens, Crystalline / Q000196:embryology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D013997:Time Factors
D003459:Crystallins