Prostaglandin determination with fluorescent reagents.
Fluorescent derivatives of PGE2 as a model substance were formed with naphthyl isocyanate, anthracene isocyanate, 4-(6-methyl- benzothiazol -2-yl)phenyl isocyanate, benzocoumarin -3-carboxylic acid chloride and 4-bromomethyl-7- methoxycoumarin . The optimum conditions for derivatization with these reagents were investigated and the results were compared. Quantitative determination was performed by means of fluorodensitometric measurement of the derivatives. Using 4-bromomethyl-7- methoxycoumarin as a reagent as little as 100 pg of the PGE2 derivative can be detected.
D055598:Chemical Phenomena; D002621:Chemistry; D015232:Dinoprostone; D005456:Fluorescent Dyes; D011453:Prostaglandins / Q000032:analysis*; D011458:Prostaglandins E / Q000032:analysis; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D011453:Prostaglandins; D011458:Prostaglandins E; D015232:Dinoprostone