Biological effects of trilostane in vitro on oocyte maturation and fertilization in the hamster.
The effects of the inhibition of steroidogenesis by trilostane on oocyte maturation were examined by studying spontaneous maturation and fertilization in vitro. 10(-6)M trilostane had no influence on the meiotic process, whether the oocytes were naked or not. At a concentration of 10(-6)M and 10(-7)M trilostane, low normal pronuclear formation and high polyspermy were found during in vitro fertilization. However, no retarded male pronuclear development could be detected in the trilostane-treated group. Thus, steroid producing activity within ova is apparently necessary to prevent multiple sperm penetration, but it has no effect on meiosis or the action of the so-called male pronucleus growth factor (MPGF).
D015096:3-Hydroxysteroid Dehydrogenases / Q000037:antagonists & inhibitors; D000818:Animals; D002467:Cell Nucleus / Q000502:physiology; D002478:Cells, Cultured; D006224:Cricetinae; D013196:Dihydrotestosterone / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects*; D008540:Meiosis / Q000187:drug effects; D008647:Mesocricetus; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology
D013196:Dihydrotestosterone; D015096:3-Hydroxysteroid Dehydrogenases; C009954:trilostane