Two-dimensional gel analysis of swine histocompatibility antigens.
Miniature swine MHC antigens from three inbred herds were examined by two-dimensional gel electrophoresis. These antigens were found to constitute a series of complex glycoproteins displaying haplotype-specific patterns that allowed the distinction of both class I and class II molecules among the three haplotypes. Selected outbred pig antisera reacted with a subset of class I antigens, suggesting the presence of at least two distinct molecular species among these antigens. Similarly, alloantisera reacting with mouse Ia antigens and a monoclonal anti-human DR were shown to immunoprecipitate a subset of class II molecules. Examination of the cells from two recombinant haplotypes demonstrated that both independent recombinational events took place between the class I and class II genes.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006238:Haploidy; D006649:Histocompatibility Antigens / Q000032:analysis* / Q000145:classification / Q000235:genetics; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D051379:Mice; D013552:Swine; D013556:Swine, Miniature / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006649:Histocompatibility Antigens; D000949:Histocompatibility Antigens Class II