Cultures of central neurons grown in the presence of phencyclidine.
In order to study the mode of action of phencyclidine at the cellular level differentiating cultures of dissociated nerve cells from brain and spinal cord have been used. Cells were grown in the presence of 50-500 microM of PCP or tritium labelled PCP, for the periods of 1-11 days. PCP in the concentration of 500 microM caused progressive degeneration of nerve cells, already noticeable after 24 h. No significant morphological changes were observed in cells exposed to PCP at concentrations up to 200 microM. In brain cultures exposed to 200 microM PCP, 50% decrease of AChE activity was observed. No decrease in enzyme activity was found in cultures of spinal cord.
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D010622:Phencyclidine / Q000494:pharmacology*; D051381:Rats
D000110:Acetylcholinesterase; D010622:Phencyclidine