A new technique for the determination of diethylstilboestrol.
A new method has been developed to determine diethylstilboestrol (DES) in bovine urine at the picogramme level. Ion-pair-systems are used for purification and isolation. Following formation of the electrophilic di-heptafluorobutyryl (di-HFB) derivative, identification and quantification were carried out by means of capillary gaschromatography-negative chemical ionization mass spectrometry, using CH4 or CH4-N2O 4: 1 as reagent gases.
D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004054:Diethylstilbestrol / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006868:Hydrolysis
D004054:Diethylstilbestrol