Characterization and partial purification of 'renocortins': two polypeptides formed in renal cells causing the anti-phospholipase-like action of glucocorticoids.
1 Anti-inflammatory steroids reduce prostaglandin E2 (PGE2) synthesis in rat renomedullary interstitial cells in culture by inhibiting the release of arachidonic acid from membranous phospholipid stores, exhibiting antiphospholipase-like properties. 2 After treatment of the cells with dexamethasone 10(-6)M, these cells release a protein in the supernatant. 3 This supernatant is able to inhibit PGE2 secretion in untreated cells and to inhibit phospholipase A2 activity in an in vitro system. 4 Using chromatofocusing separation, we showed that two distinct proteins exist with isoelectric points of 5.8 and 8.3. 5 Using gel permeation separation, we showed that two proteins exist with apparent molecular weights of 15,000 and 30,000 daltons. 6 We conclude that, in renal cells in culture, anti-inflammatory steroids induce the synthesis and the release of two polypeptides which we have named 'Renocortins' (induced by corticoids in renal cells) causing the antiphospholipase-like action of glucocorticoids. 7 Our results are in good agreement with others, but as renal cells are not directly involved in the inflammatory process, we suggest that this steroid-induced phenomenon is not solely involved in the inflammatory reaction but is of more general physiological relevance.
D000818:Animals; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003907:Dexamethasone / Q000494:pharmacology; D015232:Dinoprostone; D005938:Glucocorticoids / Q000494:pharmacology*; D007525:Isoelectric Focusing; D007668:Kidney / Q000378:metabolism*; D010455:Peptides / Q000302:isolation & purification* / Q000502:physiology; D010740:Phospholipases / Q000037:antagonists & inhibitors*; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D011458:Prostaglandins E / Q000378:metabolism; D051381:Rats
D005938:Glucocorticoids; D010455:Peptides; D011458:Prostaglandins E; D003907:Dexamethasone; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D015232:Dinoprostone