Growth, metastasis, immunogenicity, and chromosomal content of a nickel-induced rhabdomyosarcoma and subsequent cloned cell lines in rats.
Karyotype patterns, growth, metastasis, and immunogenicity were compared in a rhabdomyosarcoma induced by a single injection of metallic nickel with those in subsequent tumor-cloned cell lines. The primary tumor was induced in a male WAG rat by im injection of 20 mg nickel powder. The parental cell line (9-4/0) and 8 cell subpopulations (J 9-4) isolated from a primary tumor by cloning on agarose were examined. The tumor cell dose inducing tumors in 50% of the animals after sc injection (TD50) and the in vitro growth characteristics showed a marked heterogeneity between parental and cloned tumor cell lines. In vitro doubling times and saturation densities were also heterogeneous without showing a discernible relationship with TD50. Chromosome patterns of the cell lines exhibited very similar modal numbers, whereas chromosome numbers were somewhat different; neither of these exhibited any correlation with tumorigenicity. Parental cell line 9-4/0 expressed a significant degree of immunogenicity, but it did not protect against pulmonary metastasis in immunized rats. Among 6 clones studied, only clone J 9-4/2 appeared to be immunogenic and reduced metastatic spread. The relevance of the comparison between the different characteristics is discussed.
D000818:Animals; D002875:Chromosomes / Q000648:ultrastructure*; D002999:Clone Cells / Q000276:immunology / Q000648:ultrastructure; D007114:Immunization; D007621:Karyotyping; D008207:Lymphatic Metastasis; D008297:Male; D009368:Neoplasm Transplantation; D009532:Nickel / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012208:Rhabdomyosarcoma / Q000139:chemically induced / Q000503:physiopathology*
D009532:Nickel