High-performance liquid chromatographic separations on dihydroxyboryl-agarose.
This paper (which is part of a series of articles on high-performance liquid chromatographic separations on agarose columns) describes the chromatographic behaviour of catecholamines, nucleosides, aminoacylated and non-aminoacylated tRNA, and glycosylated and non-glycosylated hemoglobins on agarose matrices derivatized with m-aminophenylboronic acid. These matrices primarily bind molecules with two vicinal hydroxy groups in the cis-configuration. The possible existence of secondary electrostatic and hydrophobic interactions has been studied with the aid of diagrams showing retention time as a function of pH and buffer concentration.
D001897:Boronic Acids; D002395:Catecholamines / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D009705:Nucleosides / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis; D012346:RNA, Transfer, Amino Acyl / Q000032:analysis; D012685:Sepharose
D001897:Boronic Acids; D002395:Catecholamines; D006442:Glycated Hemoglobin; D009705:Nucleosides; D012343:RNA, Transfer; D012346:RNA, Transfer, Amino Acyl; D012685:Sepharose; C028592:3-aminobenzeneboronic acid