Improved concanavalin A-Sepharose elution by specific readsorption of glycoproteins.
Elution of bound glycoproteins from concanavalin A-Sepharose can be made more efficient by their readsorption to a Blue A agarose column (specific) and Green A agarose column (less-specific) during recycling of the elution buffer. Three lysosomal enzymes were eluted in this way with marked improvement in their specific activities, time and handling and amount of eluting ligand used.
D000327:Adsorption; D002846:Chromatography, Affinity; D004396:Coloring Agents; D006023:Glycoproteins / Q000302:isolation & purification*; D006801:Humans; D006867:Hydrolases / Q000302:isolation & purification; D007066:Iduronate Sulfatase / Q000302:isolation & purification; D007068:Iduronidase / Q000302:isolation & purification; D008024:Ligands; D008099:Liver / Q000032:analysis; D008247:Lysosomes / Q000201:enzymology; D012685:Sepharose / Q000031:analogs & derivatives
D004396:Coloring Agents; D006023:Glycoproteins; D008024:Ligands; C028326:concanavalin A-sepharose; D012685:Sepharose; D006867:Hydrolases; D007066:Iduronate Sulfatase; C022708:N-sulfoglucosamine sulfohydrolase; D007068:Iduronidase