Human platelet phenolsulphotransferase M and P: substrate specificities and correlation with in vivo sulphoconjugation of paracetamol and salicylamide.
Human platelet phenolsulphotransferase exists in two functional forms. M and P. In this study the substrate specificity of the two forms has been further delineated by correlating activities in different individuals with various substrates. m-Tyramine, noradrenaline, adrenaline, 5-hydroxytryptamine, p-hydroxyamphetamine, isoprenaline, salbutamol and l-naphthol were all specific substrates for the M form of the enzyme. Paracetamol, a mixed substrate, was predominantly metabolized by the M form. Salicylamide at 5 microM was a substrate for the P form but became and M substrate at higher concentration. Phenol itself, a specific substrate for phenolsulphotransferase P at 10 microM, also became an M substrate at 1 mM concentration. These substrate specificities were confirmed with the selective inhibitor, dichloronitrophenol. In this study, we measured phenolsulphotransferase activity in platelets from 13 individuals selected on the basis of their wide variation in ability to sulphoconjugate paracetamol and salicylamide in vivo. There was no significant relationship between the in vivo pattern with either drug and the activity of platelet phenolsulphotransferase assayed with paracetamol or salicylamide respectively.
D000082:Acetaminophen / Q000378:metabolism*; D015239:Arylsulfotransferase; D001792:Blood Platelets / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D009596:Nitrophenols / Q000494:pharmacology; D012457:Salicylamides / Q000378:metabolism*; D013379:Substrate Specificity; D013466:Sulfurtransferases / Q000378:metabolism*
D007527:Isoenzymes; D009596:Nitrophenols; D012457:Salicylamides; D000082:Acetaminophen; C015802:2,6-dichloro-4-nitrophenol; D013466:Sulfurtransferases; D015239:Arylsulfotransferase