Extraction of enamel proteins.
Two multi-step extraction procedures were used to isolate matrix proteins from fetal bovine enamel. Using SDS-PAGE and gel isoelectric focusing it was concluded that extraction procedures influence the number of protein bands in the gel, their pI, apparent molecular weight and staining intensity.
D000818:Animals; D002417:Cattle; D003743:Dental Enamel / Q000032:analysis / Q000196:embryology; D003746:Dental Enamel Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing / Q000379:methods; D007526:Isoelectric Point; D012967:Sodium Dodecyl Sulfate
D003746:Dental Enamel Proteins; D012967:Sodium Dodecyl Sulfate