Low density lipoprotein (LDL) from male volunteers stimulated the thromboxane formation by human platelets.
LDL (0.5 - 2.0 mg LDL-cholesterol/ml) isolated from venous blood of healthy male volunteers stimulated dose-dependently the malondialdehyde (MDA) formation by frozen human platelets, used as a marker for the activity of the thromboxane synthetase. HDL (0.25 - 1.0 mg HDL-cholesterol/ml) and human serum albumin (1 - 10 mg/ml) had no concentration-dependent influence on the MDA formation. If these results can be extended to in vivo they suggested the strong connection between the prostaglandin and lipoprotein hypothesis of pathogenesis of atherosclerosis.
D000328:Adult; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008075:Lipoproteins, HDL / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008297:Male; D008314:Malonates / Q000096:biosynthesis*; D008315:Malondialdehyde / Q000096:biosynthesis*; D008875:Middle Aged; D011450:Prostaglandin Endoperoxides, Synthetic / Q000378:metabolism; D044262:Prostaglandin H2; D011463:Prostaglandins H / Q000378:metabolism; D012709:Serum Albumin / Q000494:pharmacology; D013268:Stimulation, Chemical; D013930:Thromboxane-A Synthase / Q000378:metabolism; D013931:Thromboxanes / Q000096:biosynthesis*
D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008314:Malonates; D011450:Prostaglandin Endoperoxides, Synthetic; D011463:Prostaglandins H; D012709:Serum Albumin; D013931:Thromboxanes; D044262:Prostaglandin H2; D008315:Malondialdehyde; D013930:Thromboxane-A Synthase