Spectrophotometric analyses of hydroxysteroid dehydrogenase activity in presumed steroid-producing tissues of the brook lamprey (Lampetra planeri bloch) in different developmental stages.
Hydroxysteroid dehydrogenase (HSD) activity was determined spectrophotometrically on homogenates of the pronephros, the opisthonephros, and the gonads of metamorphosing, immature, and mature adult male and female brook lampreys (Lampetra planeri Bloch), employing pregnenolone, dehydroepiandrosterone, androsterone, epiandrosterone, and 3 beta,17 beta-dihydroxy-5 alpha-androstane as substrates and NAD or NADP as coenzymes in October, December, February, and April. With NADP a higher HSD-dependent HSD activity was detected in presumed adrenocortical tissue (PAT) homogenates with pregnenolone and 3 beta,17 beta-dihydroxy-5 alpha-androstane and in gonad homogenates only with 3 beta,17 beta-dihydroxy-5 alpha-androstane. With 3 beta,17 beta-dihydroxy-5 alpha-androstane as substrate the highest enzyme activities were measured in PAT and gonad homogenates from animals of all investigated life stages. A varying intensity of HSD activity in the examined tissue homogenates probably dependent on stages of the individual development was observed.
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000302:Adrenal Cortex / Q000201:enzymology; D000728:Androgens / Q000378:metabolism; D015113:Androstane-3,17-diol / Q000378:metabolism; D000818:Animals; D005260:Female; D005399:Fishes / Q000254:growth & development*; D006066:Gonads / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D007798:Lampreys / Q000254:growth & development*; D008297:Male; D009243:NAD / Q000494:pharmacology; D009249:NADP / Q000494:pharmacology; D011284:Pregnenolone / Q000378:metabolism; D012621:Seasons; D013379:Substrate Specificity
D000728:Androgens; D009243:NAD; D015113:Androstane-3,17-diol; D009249:NADP; D011284:Pregnenolone; D015096:3-Hydroxysteroid Dehydrogenases