Immunohistochemical evidence for the coexistence of histidine decarboxylase-like and glutamate decarboxylase-like immunoreactivities in nerve cells of the magnocellular nucleus of the posterior hypothalamus of rats.
Immunohistochemical staining of alternate consecutive sections revealed numerous histidine decarboxylase (L-histidine carboxy-lyase, EC 4.1.1.22)-like immunoreactive neurons that also contained glutamate decarboxylase (L-glutamate 1-carboxy-lyase, EC 4.1.1.15)-like immunoreactive structures in the tuberal magnocellular nucleus, the caudal magnocellular nucleus, and the postmammillary caudal magnocellular nucleus of the posterior hypothalamus of rats. Furthermore, in immunohistochemical double-staining procedures, almost all neurons in the magnocellular nuclei had both histidine decarboxylase-like and glutamate decarboxylase-like immunoreactivities. These results suggest the coexistence of histamine and gamma-aminobutyric acid in single neurons in these nuclei.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D002262:Carboxy-Lyases / Q000032:analysis*; D005968:Glutamate Decarboxylase / Q000032:analysis*; D006640:Histidine Decarboxylase / Q000032:analysis*; D006651:Histocytochemistry; D007031:Hypothalamus / Q000201:enzymology*; D007034:Hypothalamus, Posterior / Q000166:cytology / Q000201:enzymology*; D008099:Liver / Q000201:enzymology; D008297:Male; D009474:Neurons / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
D000906:Antibodies; D000936:Antigen-Antibody Complex; D002262:Carboxy-Lyases; D005968:Glutamate Decarboxylase; D006640:Histidine Decarboxylase