Platelet imipramine binding in autistic subjects.
Previous reports of elevated platelet serotonin (5-HT) concentrations in autistic subjects suggest that platelet 5-HT uptake might be altered in autism. Parameters of 3H-imipramine (IMI) binding were measured in 11 drug-free autistic subjects and 10 normal volunteers. Similar means (+/- SD) for Bmax (autistics, 1350 +/- fmole/mg protein; normals, 1590 +/- 206 fmole/mg protein) and Kd (autistics, 0.98 +/- 0.10 nM; normals, 0.94 +/- 0.13 nM) were found in the two groups. The normal number (Bmax) and affinity (Kd) of the IMI binding site in autistic subjects suggest that the regulation of 5-HT uptake is not different in autism.
D000293:Adolescent; D000328:Adult; D001321:Autistic Disorder / Q000097:blood*; D001792:Blood Platelets / Q000378:metabolism*; D002648:Child; D006801:Humans; D007099:Imipramine / Q000097:blood*; D012701:Serotonin / Q000097:blood; D014316:Tritium
D014316:Tritium; D012701:Serotonin; D007099:Imipramine