7-Alpha-hydroxytestosterone in seminiferous tubules of rat testis.
Production of 4-androstene-7 alpha,17 beta-diol-3-one (7 alpha-hydroxytestosterone) from testosterone was measured in seminiferous tubule and interstitial fractions of rat testis. In adults, specific activity of the 7 alpha-hydroxylase was about 10 times higher in interstitial cells than in seminiferous tubules, but tubule production was a significant portion of the total. Seminiferous tubule 7 alpha-hydroxylase was not detectable in weanlings. Also, we confirmed that 7 alpha-hydroxytestosterone inhibits the activity of the 5 alpha-reductase and 3 alpha-hydroxysteroid dehydrogenase active on testosterone and suggest a role in maturational changes.
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000378:metabolism; D006915:Hydroxytestosterones / Q000032:analysis*; D008297:Male; D012671:Seminiferous Tubules / Q000032:analysis*; D013737:Testis / Q000032:analysis*; D013739:Testosterone / Q000031:analogs & derivatives*; D014886:Weaning
D006915:Hydroxytestosterones; C032278:4,17 beta-dihydroxy-4-androstene-3-one; D013739:Testosterone; D015096:3-Hydroxysteroid Dehydrogenases; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase