Incorporation of inorganic [35S]-sulphate into glycoproteins of rat buccal and palatal minor salivary glands in vivo and in vitro.
The incorporation of sodium [35S]-sulphate into the buccal and palatal minor glands of the albino rat was studied in vivo and in vitro. The biosynthesis of 35S-labelled macromolecules possessing the characteristics of sulphated glycoproteins was evident in both. Uptake of [35S]-sulphate into the palatal tissue in vivo was more rapid (approx. 10-fold at 2 h) and produced compounds of higher specific activity by comparison with buccal extracts. Following in-vitro culture in the presence of [35S]-sulphate, the 35S-labelled glycoproteins secreted into the medium contained a greater proportion of lower molecular-weight products and were of higher specific activity (in the range 10-20-fold greater) than those in the tissue fraction. No sulphated glycosaminoglycans were apparent in any of the products isolated.
D000818:Animals; D002610:Cheek; D051152:Clusterin; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D066298:In Vitro Techniques; D018832:Molecular Chaperones; D010159:Palate; D051381:Rats; D012469:Salivary Glands / Q000378:metabolism*; D012470:Salivary Glands, Minor / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000302:isolation & purification / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism*
D051152:Clusterin; D006023:Glycoproteins; D018832:Molecular Chaperones; D012471:Salivary Proteins and Peptides; D013431:Sulfates; C012036:sodium sulfate