Effect of streptozotocin-induced diabetes on bile acid sulfation in male rat liver.
The sulfation of bile acids is hormone dependent, being increased in females and ethynylestradiol (EE)-treated males compared with normal males. Diabetes causes significant alterations in estrogen metabolism and uterine estrogen receptor kinetics. Male rats were given streptozotocin (90 mg/kg) and diabetes was verified. An increase in hepatic bile acid sulfotransferase (BAST) activity was significant by 6 days and continued to increase to 29 days. This increase was prevented by insulin replacement. Administration of EE (6.0-600 micrograms X kg-1 X day-1) to normal male rats resulted in a significant increase in hepatic BAST activity; however, administration of similar doses of EE to diabetic males failed to further increase activity levels over the already-elevated levels in the diabetic controls. This increase in in vitro specific activity was accompanied by an increase in the biliary excretion of lithocholate 3-sulfate and taurolithocholate 3-sulfate in 21-day-diabetic animals. Bile flow and total bile acid excretion were also markedly increased in the diabetic animals. The data indicate that streptozotocin-induced diabetes causes a significant increase in hepatic BAST activity. These findings are consistent with an alteration in hepatic estrogen action in streptozotocin-induced diabetes.
D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004997:Ethinyl Estradiol / Q000494:pharmacology; D008095:Lithocholic Acid / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000378:metabolism; D013658:Taurolithocholic Acid / Q000378:metabolism
D001647:Bile Acids and Salts; C020980:bile acid sulfates; D004997:Ethinyl Estradiol; D013658:Taurolithocholic Acid; D008095:Lithocholic Acid; D013466:Sulfurtransferases; D015238:Sulfotransferases; C019786:bile-salt sulfotransferase