Inhibition of dimethylsulfoxide-induced differentiation in Friend erythroleukemic cells by diacylglycerols and phospholipase C.
The effects of diacylglycerols and phospholipase C on dimethylsulfoxide (DMSO)-induced differentiation were investigated in Friend erythroleukemic cells (FELC). Greater than 80% of cells become benzidine-positive when incubated with 1.5% DMSO. The tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), inhibits DMSO-induced differentiation in FELC. Diacylglycerols were found to inhibit DMSO-induced differentiation dose responsively with the order of potency being 1-oleoyl-2-acetylglycerol (OAG) greater than dicaprylin greater than dilaurin greater than diolein. Phospholipase C which releases endogenous diacylglycerols from membrane phospholipids also inhibited DMSO-induced differentiation dose responsively. These results support the hypothesis that diacylglycerols can have effects similar to tumor promoters and suggest that protein kinase C may be a common mechanism for tumor promotion.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004075:Diglycerides / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005622:Friend murine leukemia virus; D005989:Glycerides / Q000494:pharmacology*; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D007942:Leukemia, Experimental / Q000473:pathology*; D051379:Mice; D010740:Phospholipases / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D010738:Type C Phospholipases / Q000494:pharmacology*
D004075:Diglycerides; D005989:Glycerides; D010740:Phospholipases; D010738:Type C Phospholipases; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide