Enzymatic sulfation of steroids. XVIII. study of the specific estradiol-17 beta sulfotransferase of rat liver cytosol, that converts the estrogen to its 3-sulfate, and some elements of the endocrine control of its production.
A radioisotopic assay for cytoplasmic estradiol-17 beta sulfotransferase activity in rat liver was developed. Routine enzyme assays used 120 microM [3H]estradiol-17 beta, 240 microM 3'-phosphoadenosine-5'-phosphosulfate, and enzyme samples containing up to 0.60 mg of cytosol protein. Livers from males and females sulfated 934 +/- 231 and 861 +/- 266 nmol estradiol-17 beta . h-1 . g-1. DEAE-Sephadex A-50 chromatography showed that most of the cytoplasmic enzyme activity eluted as one peak that was well separated from glucocorticoid and 3 beta-hydroxysteroid sulfotransferases. Pooled column fractions containing this estradiol-17 beta sulfotransferase exhibited kinetic properties similar to the enzyme activity in cytosol, but gave slightly greater activity with 180 microM estradiol-17 beta and 360 microM 3'-phosphoadenosine-5'-phosphosulfate. Apparent Km's for the steroid and the coenzyme were 71-85 and 80-93 microM, respectively. The pH optimum for the enzyme reaction was 7.75 +/- 0.25. The enzyme sulfated estradiol-17 beta at all concentrations tested between 10 and 180 microM. It did not sulfate estrone, testosterone, dehydroepiandrosterone, or cortisol well at any test concentration between 10 and 120 microM. The sulfation product was estra-1,3,5-triene-17 beta-ol-3-sulfate. The molecular weight of the enzyme was 54 500 +/- 2300 by Sephadex G-100 chromatography. The estradiol-17 beta sulfotransferase was inhibited strongly by phenols, but not by corticosterone, deoxycorticosterone, dehydroepiandrosterone, estrone, progesterone, or testosterone. Adrenalectomy diminished the estradiol-17 beta sulfotransferase activity greatly, owing to decreases of the specific estradiol-17 beta sulfotransferase concentration. The possible relationships between the specific estradiol-17 beta sulfotransferase and other sulfotransferases in rat liver are discussed.
D000311:Adrenal Glands / Q000502:physiology*; D000818:Animals; D003600:Cytosol / Q000201:enzymology; D004958:Estradiol / Q000031:analogs & derivatives / Q000378:metabolism*; D005260:Female; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D008970:Molecular Weight; D010636:Phenols / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013256:Steroids / Q000494:pharmacology; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000302:isolation & purification / Q000378:metabolism*
D010636:Phenols; D013256:Steroids; C028628:estradiol-3-sulfate; D004958:Estradiol; D013466:Sulfurtransferases; D015238:Sulfotransferases; C037564:estradiol-17 beta sulfotransferase