DNA of Chinese hamster V79 cells newly synthesized after ultraviolet irradiation contains alkali-labile sites.
Pulse-labeled daughter DNA of UV-irradiated Chinese hamster V79 cells was denatured in alkaline or neutral conditions and analysed by sucrose gradient centrifugation. A comparison of the sedimentation profiles of DNA treated in alkaline or neutral conditions has shown that in UV-irradiated cells some alkali-labile sites are produced during replication of damaged templates.
D000468:Alkalies; D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000096:biosynthesis / Q000528:radiation effects*; D004260:DNA Repair / Q000528:radiation effects*; D009691:Nucleic Acid Denaturation; D014466:Ultraviolet Rays
D000468:Alkalies; D004247:DNA