Origin of the duplicated ribonuclease gene in guinea-pig: comparison of the amino acid sequences with those of two close relatives: capybara and cuis ribonuclease.
The amino acid sequences of the pancreatic ribonuclease from capybara (Hydrochoerus hydrochaeris) and cuis (Galea musteloides) were determined. Both species belong to the same superfamily of the hystricomorph rodents as the guinea-pig. In guinea-pig pancreas two ribonucleases are present as a result of a recent gene duplication, but in capybara and cuis pancreas only one single ribonuclease has been found. A most parsimonious tree of ribonucleases indicates that the gene duplication leading to both guinea-pig ribonucleases occurred before the divergence of guinea-pig from capybara and cuis. This would mean that changes in expression of the ribonuclease genes have occurred in these taxa. Cuis and capybara ribonuclease have no Asn-X-Ser/Thr sequences and are carbohydrate-free proteins. Capybara ribonuclease has leucine at position 114, a position occupied by proline in the cis-configuration in bovine pancreatic ribonuclease.
D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D006168:Guinea Pigs / Q000235:genetics*; D005810:Multigene Family; D010179:Pancreas / Q000201:enzymology; D012260:Ribonucleases / Q000235:genetics*; D012377:Rodentia / Q000235:genetics
D012260:Ribonucleases