Comparison of the physical characteristics of the molybdate-stabilized glucocorticoid receptor from rat, pig and human tissues.
The molybdate-stabilized glucocorticoid receptor in cytosol preparations from rat, pig and human thymus, rat liver and CEM-C7 human leukaemic lymphoblasts was characterized as its complex with [1,2,4-3H]triamcinolone acetonide, by gel filtration on Sephacryl S-300 and by ultracentrifugation on sucrose density gradients. At low ionic strength, the complex from all sources was detected as a species with a sedimentation coefficient of 9.1-9.6S and a Stokes radius of 5.70-5.80 nm (form I); this form is believed to represent the non-transformed, undegraded complex. Exposure to 0.4 M KCl in the presence of molybdate resulted, in all cases, in the generation of a smaller form (form II) with a sedimentation coefficient of 4.5-4.8S and a Stokes radius of 4.70-4.77 nm. Form II has been shown to possess properties characteristic of the transformed (activated) state. It is concluded that the glucocorticoid receptor shows pronounced interspecies and intertissue similarities, and that pig thymus may represent a convenient source of tissue for glucocorticoid receptor purification.
D000818:Animals; D002460:Cell Line; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002648:Child; D002850:Chromatography, Gel; D006801:Humans; D007945:Leukemia, Lymphoid / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008982:Molybdenum; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000302:isolation & purification*; D011987:Receptors, Steroid / Q000302:isolation & purification*; D013045:Species Specificity; D013552:Swine; D013950:Thymus Gland / Q000378:metabolism
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D008982:Molybdenum; C024687:sodium molybdate(VI)