[Spectrophotometric method of evaluating tumor cell agglutination induced by lectins].
The lectin (PHA and Con A) induced agglutination of a human tumour cell line (lung adenocarcinoma and osteosarcoma) was estimated by the spectrophotometric method. A decrease in the optic density for 1 min (delta D) of cell suspension and the time (t0) necessary for a complete sedimentation of cells (at delta D = 0) were used as quantitative indicators of agglutination. An increase in the concentration of tumour cells and lectins resulted in an increase of delta D and a decrease of t0. The results of spectrophotometry were correlated with the microscopic study data for tumour cells agglutination.
D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology; D000371:Agglutination; D002449:Cell Aggregation; D002452:Cell Count; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000276:immunology / Q000473:pathology; D009369:Neoplasms / Q000276:immunology / Q000473:pathology*; D012516:Osteosarcoma / Q000276:immunology / Q000473:pathology; D010835:Phytohemagglutinins / Q000494:pharmacology; D013053:Spectrophotometry
D037102:Lectins; D010835:Phytohemagglutinins; D003208:Concanavalin A
Spektrofotometricheskiui metod otsenki aggliutinatsii opukholevykh kletok, vyzyvaemykh lektinami.