An autoradiographic study of the in-vivo incorporation of [3H]-palmitic acid into the dentine and enamel lipids of rat incisors, with a comparison of rapid-freezing freeze-substitution fixation and aldehyde fixation.
Four and twenty-four hours after injection of the labelled precursor, there were twice as many silver grains scored with the cryotechniques as with conventional fixation. Absence of labelled-band formation in dentine, the diffusion of silver throughout the entire thickness of dentine as well as the layer of forming enamel. This suggests that lipids are membrane-associated components rather than true matrix-associated components.
D000447:Aldehydes / Q000494:pharmacology; D000818:Animals; D001345:Autoradiography; D003743:Dental Enamel / Q000378:metabolism*; D003804:Dentin / Q000378:metabolism*; D005615:Freezing; D050356:Lipid Metabolism; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014021:Tissue Preservation
D000447:Aldehydes; D010169:Palmitic Acids; D019308:Palmitic Acid