Fluorescence studies using synchrotron radiation on normal and differentiated cells labeled with parinaric acids.
Changes in membrane properties during the differentiation process in K562 cells have been investigated. A decrease of lectin-induced agglutination has been detected. The agglutination assay revealed to be an early and sensitive test to monitor the induced differentiation of the K562 cells. Naturally occurring fluorescent fatty acids (cis- and trans-parinaric acids) and the recently developed multifrequency phase and modulation technique were used to study cell membrane properties. Changes in fluorescence lifetime and polarization are clearly associated with cell differentiation, suggesting the involvement of the cellular plasma membrane in the differentiation process.
D002449:Cell Aggregation; D002454:Cell Differentiation; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D005231:Fatty Acids, Unsaturated; D005456:Fluorescent Dyes; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute; D013050:Spectrometry, Fluorescence
D005231:Fatty Acids, Unsaturated; D005456:Fluorescent Dyes; D006454:Hemoglobins; C013641:parinaric acid