Amplified C lambda and c-abl genes are on the same marker chromosome in K562 leukemia cells.
The human leukemia cell line K562, derived from a patient with Philadelphia chromosome-positive chronic myelogenous leukemia, contains amplified c-abl oncogenes and unrearranged C lambda genes. Using in situ hybridization techniques, we have determined that the amplified c-abl and C lambda DNA sequences of K562 cells are both located on the same abnormal acrocentric marker chromosome, which may represent an altered Philadelphia chromosome.
D002460:Cell Line; D002871:Chromosome Banding; D002904:Chromosomes, Human, 21-22 and Y; D005784:Gene Amplification; D006801:Humans; D007621:Karyotyping; D007945:Leukemia, Lymphoid / Q000235:genetics*; D008677:Metaphase; D009693:Nucleic Acid Hybridization; D009857:Oncogenes