Electrical activity of neurons cultured in vivo.
A method is presented, where glass microchambers (1 X 2 mm) are implanted in various brain regions of 4-10 days old rats. The microchamber contains a collagen coated coverglass with cell material taken from the same region of a littermate. Investigation of the neural growth occurs after 4-14 days, on an inverted phase contrast microscope in a temperature and pH controlled, oxygenated BSS medium. Various growth geometries of neurons are observed and microelectrode recordings reveal spontaneous and electrically evoked spike and complex activity.
D000818:Animals; D001933:Brain Stem / Q000166:cytology; D002455:Cell Division; D002478:Cells, Cultured; D008722:Methods; D009474:Neurons / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors