New synthetic and natural tuftsin-related compounds and evaluation of their phagocytosis-stimulating activity.
The preparation of large amounts of tuftsin (50 g) as well as purification by preparative HPLC are presented. Synthesis of several analogues exhibiting significant phagocytosis-stimulating activity of human PMN are described and discussed. On the other hand, the synthesis of a natural human IgG decapeptide fragment 335-344 that could be liberated from the same carrier molecule is presented. This decapeptide fragment, Thr-Ile-Ser-Lys-Ala-Lys-Gly-Gln-Pro-Arg, showed interesting activity in stimulating phagocytosis of human polymorphonuclear leukocytes.
D000595:Amino Acid Sequence; D006801:Humans; D007074:Immunoglobulin G; D008722:Methods; D009504:Neutrophils / Q000502:physiology*; D009842:Oligopeptides / Q000138:chemical synthesis; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology; D010587:Phagocytosis; D014405:Tuftsin / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000494:pharmacology
D007074:Immunoglobulin G; D009842:Oligopeptides; D010446:Peptide Fragments; D014405:Tuftsin