Microscopy of the dentine of enamel-free areas of rat molar teeth.
Dentine of the enamel-free areas (EFA) of first mandibular molar teeth of rats aged between 20-150 days was examined by scanning electron microscopy. Demineralized sections were stained with Gram-Twort's stain and examined for dental caries. In newly erupted teeth, a few dentinal tubules opened on the EFA surface. As teeth became worn down, extensive areas of EFA showed numerous patent tubules. In primary dentine, no occlusion or partial occlusion of tubules was apparent at any age except at the occlusal surface. It is postulated that this surface narrowing was caused either by dentine debris or salivary deposits. The only other covering of tubule openings appeared to be a thin salivary pellicle which in older animals was plaque-like. Patent tubules in primary dentine contained odontoblast processes which even in worn teeth extended to EFA surfaces. Processes in adjacent enamel-covered dentine also extended to the enamel-dentine junction. Only a few examples of bacterial invasion of EFA dentine were seen: in younger animals bacteria occupied cracks in the EFA surface, but in older animals they also occupied tubules. The EFA dentine does not appear to respond to attrition by infilling of the tubules; the persistence of vital odontoblast processes in worn teeth suggests that dead tract formation is not extensive. Continuous occlusal wear may restrict the progress of caries in EFA but odontoblasts in the rat may be also physiologically and immunologically involved in restricting the ingress of bacteria into otherwise poorly protected EFA.
D000367:Age Factors; D000818:Animals; D044622:Dental Pellicle; D003804:Dentin / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D008963:Molar / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D014072:Tooth Abrasion / Q000473:pathology*