The target sizes of the in situ and solubilized forms of human placental steroid sulfatase as measured by radiation inactivation.
Whole microsomal membrane preparations and Triton X-100-solubilized human placental steroid sulfatase were subjected to radiation inactivation analysis using gamma rays from a 60Co irradiator in order to assess the size of the physiological form of the enzyme. The data indicate that the enzyme exists as a monomer of molecular weight 78600 in Triton-containing buffers and as a polymer of molecular weight 533000 within the microsomal membrane.
D005260:Female; D006801:Humans; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology / Q000528:radiation effects*; D008970:Molecular Weight; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D043266:Steryl-Sulfatase; D013429:Sulfatases / Q000378:metabolism / Q000528:radiation effects*
D013429:Sulfatases; D043266:Steryl-Sulfatase