Human monoclonal antibody to a neuroectodermal tumor antigen (OFA-I-2).
Human IgM kappa monoclonal antibody to human tumors of neuroectodermal origin was produced in the spent medium of an Epstein-Barr virus-transformed B-lymphoblastoid cell line, L72. Chemically, the antigen was identified as ganglioside GD2 [Gal NAc beta 1----4 (Neu Ac alpha 2----8 Neu Ac alpha 2---3) Gal beta 1----4 Glc----ceramide]. Twenty-seven mg of pure human IgM were obtained from 10 liters of L72 spent medium using salt and hypotonic precipitation, ultracentrifugation, and Sephacryl-S 300 superfine gel filtration. The monoclonal origin of the antibody was determined by agarose isoelectrofocusing. This human monoclonal antibody may be a particularly useful reagent for immunotherapy trials in cancer patients.
D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology*; D001943:Breast Neoplasms / Q000276:immunology; D002460:Cell Line; D003110:Colonic Neoplasms / Q000276:immunology; D005910:Glioma / Q000276:immunology; D006801:Humans; D007680:Kidney Neoplasms / Q000276:immunology; D007945:Leukemia, Lymphoid / Q000276:immunology; D008175:Lung Neoplasms / Q000276:immunology; D008545:Melanoma / Q000276:immunology; D009447:Neuroblastoma / Q000276:immunology; D012509:Sarcoma / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; C031192:oncofetal antigens