Location of an essential carboxyl group along the heavy chain of cardiac and skeletal myosin subfragments 1.
Cardiac and skeletal myosin subfragments 1 cleaved into three fragments were modified by 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide metho-p-toluene-sulfonate in the presence of the nucleophile nitrotyrosine ethyl ester. The effects observed (first-order kinetics of ATPase inactivation, incorporation of 1 mol of nitrotyrosine/mol of subfragment 1) were similar to those previously observed for the nondigested subfragments 1 [Lacombe, G., Van Thiem, N., & Swynghedauw, B. (1981) Biochemistry 20, 3648-3653; Körner, M., Van Thiem, N., Lacombe, G., & Swynghedauw, B. (1982) Biochem. Biophys. Res. Commun. 105, 1198-1207]. For both native and digested subfragments 1, which were inactivated to the extent of about 70%, the location of the label nitrotyrosine was performed by immunological blotting with 125I-labeled anti-nitrotyrosine immunoglobulins. It was found that the modified residue was essentially located on the heavy chain for the native subfragments 1 and on the 50K peptide for the digested subfragments 1.
D000818:Animals; D003028:CME-Carbodiimide / Q000031:analogs & derivatives / Q000494:pharmacology; D004285:Dogs; D009132:Muscles / Q000032:analysis*; D009206:Myocardium / Q000032:analysis*; D015879:Myosin Subfragments; D009218:Myosins / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000494:pharmacology
D015879:Myosin Subfragments; D010446:Peptide Fragments; D003028:CME-Carbodiimide; D014443:Tyrosine; C024960:nitrotyrosine ethyl ester; D009218:Myosins