Thiol reactivity of the nitrogenase Fe-protein from Azotobacter vinelandii.
A procedure has been developed to examine some of the functional roles of the 14 cysteinyl residues in the nitrogenase Fe-protein (Av2) from Azotobacter vinelandii. The reduced form of Av2 was alkylated with iodo[2-14C]acetic acid under a variety of experimental conditions, e.g. reaction in the presence of nucleotides, alpha,alpha'-dipyridyl and nucleotides, or denaturants. The labeled cysteinyl residues were identified and quantified using an analytical DEAE-Sepharose ion exchange chromatography peptide mapping technique based upon the known amino acid sequence (Hausinger, R. P., and Howard, J. B. (1982) J. Biol. Chem. 257, 2483-2490). From the results of the labeling experiments, the following features of the Av2 structure have been proposed. 1) Av2 contains no disulfides, hyperreactive thiols, or surface thiols as defined by reaction with iodoacetic acid. 2) Cysteines 97 and 132 are the probable ligands for the Av2 Fe:S center which is bound symmetrically between subunits. 3) MgATP partially protects cysteine 85 from carboxymethylation by iodoacetic acid and may be part of the nucleotide-binding site. 4) Of the five nonligand thiols only cysteines 5 and 184 are completely alkylated when Av2 is denatured in hexamethylphosphoramide, whereas all five nonligand thiols appear to rapidly exchange at the Fe:S center if the protein is denatured in the absence of alkylating reagents. 5) Both Av2 and apo-Av2 appear to undergo a reversible conformational change upon binding MgATP.
D001395:Azotobacter / Q000201:enzymology*; D002250:Carbon Radioisotopes; D003545:Cysteine / Q000032:analysis*; D007461:Iodoacetates; D019807:Iodoacetic Acid; D009591:Nitrogenase / Q000378:metabolism*; D010088:Oxidoreductases; D011485:Protein Binding; D013045:Species Specificity
D002250:Carbon Radioisotopes; D007461:Iodoacetates; D010088:Oxidoreductases; D009591:Nitrogenase; C029130:nitrogenase reductase; D003545:Cysteine; D019807:Iodoacetic Acid