Application of in vitro autoradiography for monitoring drug response in cancer chemotherapy.
Primary cultures of human breast carcinoma cells were treated for 24 hours with different concentrations of anticancer drugs followed by labelling with 3H-thymidine and 3H-uridine and subsequent autoradiographic processing to study the effect of drugs on nucleic acid synthesis in these cells. 3H-uridine autoradiography was more sensitive than 3H-thymidine autoradiography in the assessment of lethal effects. The results showed that individual neoplasms varied in drug response. This method may be useful for predicting the effectiveness of drugs for treatment of individual cases and also for screening new drugs for anticancer activity.
D000970:Antineoplastic Agents; D001345:Autoradiography; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D005260:Female; D006801:Humans; D008727:Methotrexate / Q000627:therapeutic use; D008854:Microscopy, Electron; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism; D014750:Vincristine / Q000627:therapeutic use
D000970:Antineoplastic Agents; D014750:Vincristine; D013936:Thymidine; D014529:Uridine; D008727:Methotrexate