Vascular responses of isolated canine intermediate auricular artery to vasoactive substances.
The stainless steel cannula inserting method was used to observe the effects of 7 vasoactive substances in the isolated intermediate auricular artery of the dog. The isolated artery was suspended in a bath at 37 degrees C and perfused with Ringer-Locke solution under a constant flow rate. Drugs were administered in the endothelial side of the artery through a cannulated tubing, and the response was obtained as changes in perfusion pressure. 5-Hydroxytryptamine (5-HT), norepinephrine (NE), tyramine, ATP and prostaglandin F2 alpha (PGF2 alpha) increased perfusion pressure in a dose-related manner. The order of potency for inducing vasoconstriction was 5-HT greater than NE = PGF2 alpha greater than tyramine greater than ATP greater than KCl. Histamine caused either slight biphasic responses or no change in perfusion pressure. The canine auricular artery would be a good in vitro model for studying pharmacological responses of cutaneous vessels.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002404:Catheterization; D015237:Dinoprost; D004285:Dogs; D004423:Ear / Q000098:blood supply; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009638:Norepinephrine / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology; D014439:Tyramine / Q000494:pharmacology; D014661:Vasoconstriction / Q000187:drug effects
D011460:Prostaglandins F; D012701:Serotonin; D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D015237:Dinoprost; D009638:Norepinephrine; D014439:Tyramine