Continuous extraction of urinary anthracycline antitumor antibiotics with the horizontal flow-through coil planet centrifuge.
Extraction of doxorubicin (adriamycin) and daunorubicin and their metabolites from human urine was attempted utilizing the horizontal flow-through coil planet centrifuge. Partition coefficients of the drugs for various combinations of non-aqueous phases and aqueous salt solutions were determined. Optimal coefficients for adriamycin and daunorubicin were achieved with n-butanol-0.3 M disodium hydrogen phosphate. Extraction efficiencies of the drugs from human urine comparable to those obtained by standard resin column techniques could be realized by employing the n-butanol-urine (containing 0.3 M disodium hydrogen phosphate) system in the coil planet centrifuge, at flow-rates of 500-600 ml/h, and at 650 rpm revolutional speed. Small quantities of drugs and metabolites could be continuously concentrated into small volumes of the n-butanol phase from large volumes of salted urine. The versatility of the technique was demonstrated by its application to extraction of aclacinomycin A, a novel anthracycline antitumor agent, and its metabolites from human urine.
D020001:1-Butanol; D015250:Aclarubicin; D000903:Antibiotics, Antineoplastic / Q000652:urine*; D000440:Butanols; D002498:Centrifugation / Q000295:instrumentation; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003630:Daunorubicin / Q000652:urine*; D004317:Doxorubicin / Q000652:urine*; D006801:Humans; D009279:Naphthacenes / Q000652:urine
D000903:Antibiotics, Antineoplastic; D000440:Butanols; D009279:Naphthacenes; D015250:Aclarubicin; D004317:Doxorubicin; D020001:1-Butanol; D003630:Daunorubicin