[Derivation of a mutant (HPRT-) cell line based on K-562 human erythromyeloleukemia].
A mutant (HPRT-) cell line was obtained by a series of passages in the medium with 8-azaguanine on the basis of human erythromyeloleukemia K-562 cells. Rapid total death of the cells in a selective medium containing hypoxanthine, aminopterine and thymidine (3 to 4 passages) supported the deficiency as regards hypoxanthine phosphoribosyltransferase. Preliminary experiments aimed at cytotoxicity studies demonstrated that the new mutant cell line lost the property, common to the parent K-562 line, of being sensitive to the attack of natural killers.
D001375:Azaguanine; D002460:Cell Line; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000172:deficiency; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology; D009154:Mutation
D007041:Hypoxanthine Phosphoribosyltransferase; D001375:Azaguanine
Poluchenie mutantnoĭ (GFRT-) linii kletok na osnove chelovecheskogo éritromieloleĭkoza K-562.