High-performance liquid chromatography of alpha-keto acids in human saliva.
alpha-Keto acids in human mixed saliva collected without stimulation were analysed by reversed-phase ion-pair high-performance liquid chromatography (HPLC). Several alpha-keto acids were found in saliva and their concentrations were: alpha-ketoglutaric acid (KGA), 221 +/- 142; pyruvic acid (PA), 7490 +/- 5600; alpha-ketoisovaleric acid (KIVA), 61 +/- 23; alpha-ketoisocaproic acid (KICA), 137 +/- 79; alpha-keto-beta-methylvaleric acid (KMVA), 41 +/- 19 nmol/dl (mean +/- SD, n = 40). Their levels proved to be lower than those in plasma, except that of PA. Their concentrations in saliva showed individual variation compared with those in blood.
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D045782:Hemiterpenes; D006801:Humans; D007651:Keto Acids / Q000032:analysis*; D007656:Ketoglutaric Acids / Q000032:analysis; D011773:Pyruvates / Q000032:analysis; D019289:Pyruvic Acid; D012463:Saliva / Q000032:analysis*
D045782:Hemiterpenes; D007651:Keto Acids; D007656:Ketoglutaric Acids; D011773:Pyruvates; C016211:alpha-keto-beta-methylvaleric acid; C001505:alpha-ketoisovalerate; C013082:alpha-ketoisocaproic acid; D019289:Pyruvic Acid