Comparison of Corynebacterium parvum with Freund's complete adjuvant as a potentiator of the immune response to beta-human chorionic gonadotropin linked to tetanus toxoid.
Corynebacterium parvum was compared with Freund's complete adjuvant (FCA) for potentiation of the rabbit immune response to beta-human chorionic gonadotropin linked to tetanus toxoid (beta-hCG-TT). With each adjuvant, antibodies to hCG were detected using passive hemagglutination. Higher antibody titers were produced by FCA-treated animals. The ability of antisera to beta-hCG-TT to neutralize the biological action of native hCG was determined by the rat uterine weight assay. Anti-beta-hCG-TT sera from C. parvum-treated rabbits were not significantly different (p greater than 0.10) from FCA potentiated anti-beta-hCG-TT sera in neutralizing hCG-induced uterine weight gain. C. parvum has potential for future application in active immunization studies of fertility regulation.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D006063:Chorionic Gonadotropin / Q000276:immunology*; D005260:Female; D005620:Freund's Adjuvant / Q000494:pharmacology*; D006386:Hemagglutination Tests; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D008297:Male; D009929:Organ Size; D011425:Propionibacterium acnes / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013745:Tetanus Toxoid / Q000276:immunology*; D014599:Uterus / Q000033:anatomy & histology
D000276:Adjuvants, Immunologic; D006063:Chorionic Gonadotropin; D000949:Histocompatibility Antigens Class II; D013745:Tetanus Toxoid; D005620:Freund's Adjuvant