Effect of anionic amphophiles on erythrocyte properties.
This preliminary study describes effects of two pharmacologic agents on erythrocyte behavior. Increased erythrocyte aggregation has been proposed as important in the pathogenesis of a number of disorders, but the exact mechanism by which it plays a role in disease production remains unclear. Several anionic amphophiles have been reported to benefit diabetic vascular disease and atherosclerosis. If anionic amphophiles enter the erythrocyte plasma membrane they can increase its negative charge, reducing the energy of attraction between red blood cells and diminishing erythrocyte aggregation. Erythrocytes were studied after suspension in phosphate-buffered saline containing dextran as an aggregation-promoting agent. A marginal reduction of the suspension's viscosity was found at low shear rate when 2,5- dihydroxybenzene sulfonate was added. Additionally, erythrocyte sedimentation rate was marginally influenced. Both dihydroxybenzene sulfonate and acetylsalicylate protected human erythrocytes from hemolysis at concentrations from 10(-3) to 10(-5) M. The removal of erythrocyte sialic acid using neuraminidase to reduce surface negative charge led to unequivocal interference with aggregation (MAI technique of CHIEN et al., J. Gen. Physiol., 1973) by both anionic amphophiles were studied. Dihydroxybenzene sulfonate and actylsalicylate reduced the aggregation propensity of sialic-free erythrocytes, suggesting that the effect on the low shear rate viscosity of sialic acid-containing erythrocytes, though modest, is real.
D001241:Aspirin / Q000494:pharmacology*; D001557:Benzenesulfonates / Q000494:pharmacology*; D001799:Blood Sedimentation; D001809:Blood Viscosity / Q000187:drug effects; D002123:Calcium Dobesilate / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects*; D006461:Hemolysis; D006801:Humans; D009439:Neuraminidase / Q000378:metabolism; D009997:Osmotic Pressure; D012794:Sialic Acids / Q000097:blood
D001557:Benzenesulfonates; D012794:Sialic Acids; D002123:Calcium Dobesilate; D009439:Neuraminidase; D001241:Aspirin