Specific binding of 15 HETE to lymphocytes. Effects on the fluidity of plasmatic membranes.
Specific binding of mouse lymphocytes for 15 HETE was examined by incubating cells with [14C]-15 HETE, 1 X 10(-8) to 1 X 10(-10)M. It was observed that the specific binding of radiolabeled 15 HETE is a function of time, of temperature and is modified by Ca2+ and dithiothreitol. When a fluorescent probe was embedded in the phospholipid core of the lymphocyte membrane and its motion analysed by fluorescence polarization, it was observed that 15 HETE increases the viscosity of the plasmatic membrane.
D000818:Animals; D001095:Arachidonic Acids / Q000097:blood*; D002118:Calcium / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D005454:Fluorescence Polarization; D006893:Hydroxyeicosatetraenoic Acids; D008214:Lymphocytes / Q000378:metabolism*; D008560:Membrane Fluidity; D051379:Mice; D013696:Temperature; D013997:Time Factors
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D002118:Calcium; D004229:Dithiothreitol