Nonspecific esterases of leukemia cell lines: evidence for activation of myeloid-associated zymogens in HL-60 by phorbol esters.
Myeloid leukemia cell line HL-60 contains fluoride-sensitive, myeloid-associated isoenzymes of nonspecific esterase that increase in activity when cultures are treated with phorbol ester. These isoenzymes are not detectable in B-lymphoblast cell line KLM-2, either in control or in phorbol-ester-treated cultures. No increased de novo synthesis of the isoenzymes is detectable in HL-60 treated with phorbol ester. The data suggest stimulated conversion of a preformed, myeloid-associated zymogen in HL-60.
D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D004792:Enzyme Precursors / Q000378:metabolism*; D004950:Esterases / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000201:enzymology*; D010704:Phorbols / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D004792:Enzyme Precursors; D007527:Isoenzymes; D010704:Phorbols; D004950:Esterases; D013755:Tetradecanoylphorbol Acetate