Human leukemic cells contain transforming growth factor.
A transforming growth factor was found in the extracts of leukemic cells obtained from the peripheral blood of 11 patients with leukemia. This factor stimulated the colony formation of anchorage-dependent BALB/c 3T3 cells in soft agar. The high levels of colony-stimulating activities were observed in the cell extracts from patients with chronic myelogenous leukemia in blastic crisis (CML BC), acute myelogenous leukemia and CML in chronic phase. The factor from a CML BC patient was heat- and acid-labile, relatively stable to dithiothreitol treatment and inactivated by pronase treatment. Molecular size of the factor seems more than 10,000 daltons.
D000143:Acids; D000362:Agar; D000818:Animals; D002457:Cell Extracts; D002460:Cell Line; D002469:Cell Separation; D004229:Dithiothreitol; D006358:Hot Temperature; D006801:Humans; D007938:Leukemia / Q000097:blood*; D007962:Leukocytes / Q000032:analysis*; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D015291:Transforming Growth Factors
D000143:Acids; D002457:Cell Extracts; D010455:Peptides; D015291:Transforming Growth Factors; D000362:Agar; D004229:Dithiothreitol