Alterations in major histocompatibility complex phenotypes of mouse cloned T10 sarcoma cells: association with shifts from nonmetastatic to metastatic cells.
The T10 sarcoma, induced in a (C57BL/6J X C3HeB/-FeJ)F1 (H-2b X H-2k) mouse, grows locally (L-T10) and generates spontaneous lung metastases (M-T10). L-T10 cells were found to express the H-2b haplotype, whereas M-T10 expressed both the H-2b and H-2k haplotypes. Most L-T10 cloned cells expressed the H-2b haplotype and were not metastatic. The minority expressed both H-2k and H-2b and were metastatic. Serial transplantations of H-2k-negative clones always ended in spontaneous expression of the H-2k haplotype concomitantly with the acquisition of metastatic potency. The expressed H-2k seemed to be associated with the metastatic properties inasmuch as an H-2b-positive--H-2k-negative clone, which had lost the expressed H-2b and was temporarily H-2 negative, remained nonmetastatic until reexpression of the two haplotypes occurred. Serial transfers of H-2k-positive clones resulted in the maintenance of the expressed H-2k haplotype and the retention of metastatic capacity. A shift toward increased metastatic capacity correlated with H-2k expression occurred during serial transfers of every clone tested. Expression of major histocompatibility complex components, rather than their loss, may potentiate the metastatic capacity of tumor cells.
D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D006649:Histocompatibility Antigens / Q000235:genetics; D008175:Lung Neoplasms / Q000276:immunology / Q000556:secondary; D008285:Major Histocompatibility Complex; D051379:Mice; D008815:Mice, Inbred Strains; D009362:Neoplasm Metastasis; D010641:Phenotype; D012513:Sarcoma, Experimental / Q000235:genetics / Q000276:immunology*
D006649:Histocompatibility Antigens