Lipid peroxide, alpha-tocopherol and retinoid levels in plasma and liver of rats fed diets containing beta-carotene and 13-cis-retinoic acid.
The effect of feeding large amounts of beta-carotene and 13-cis-retinoic acid (RA) on plasma and liver levels of alpha-tocopherol, lipid peroxides and retinoids was studied. Groups of young male rats were fed semipurified diets supplemented with 0, 100 mg/kg beta-carotene, 20 and 100 mg/kg 13-cis-RA. After feeding the various diets for 11 weeks, rats were killed and the concentrations of lipid peroxides, alpha-tocopherol, and retinoids were measured in blood plasma and liver. Peroxide levels were increased and alpha-tocopherol levels were decreased in plasma as well as liver of rats fed diets containing 13-cis-RA; this effect seems to be dose dependent, beta-Carotene had no significant effect on either of the above parameters. There was a decrease in the liver and plasma concentrations of retinol in rats fed 13-cis-RA; the levels of RA were generally higher in these two groups. The results suggest that the mechanism whereby 13-cis-RA increases the tissue peroxide levels may be related to its ability to decrease alpha-tocopherol levels.
D000818:Animals; D002338:Carotenoids / Q000008:administration & dosage*; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D004224:Diterpenes; D015474:Isotretinoin; D008054:Lipid Peroxides / Q000032:analysis / Q000378:metabolism*; D008099:Liver / Q000032:analysis / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D000084562:Retinyl Esters; D014212:Tretinoin / Q000008:administration & dosage* / Q000032:analysis; D014801:Vitamin A / Q000031:analogs & derivatives / Q000032:analysis; D014810:Vitamin E / Q000032:analysis / Q000378:metabolism*; D014886:Weaning; D019207:beta Carotene
D004224:Diterpenes; D008054:Lipid Peroxides; D000084562:Retinyl Esters; D019207:beta Carotene; D014801:Vitamin A; D014810:Vitamin E; C014794:retinol palmitate; D002338:Carotenoids; D014212:Tretinoin; D015474:Isotretinoin