Glycine N-methyltransferase is a folate binding protein of rat liver cytosol.
A comparison of the amino acid compositions of one of the folate-binding proteins of rat liver cytosol, folate-binding protein-cytosol II, and that of glycine N-methyltransferase (S-adenosyl-L-methionine:glycine methyltransferase, EC 2.1.1.20) from the same source indicated a great deal of structural homology between the two proteins. Antiserum prepared against the purified folate-binding protein almost completely inactivated the enzyme activity in crude liver cytosol. Purification of glycine N-methyltransferase resulted in the separation of two enzyme species, one that contained bound folate and one that did not. Each species was homogeneous, as judged by NaDodSO4/polyacrylamide gel electrophoresis, and they migrated identically.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002352:Carrier Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D058973:Folate Receptors, GPI-Anchored; D005492:Folic Acid / Q000378:metabolism*; D050938:Glycine N-Methyltransferase; D008099:Liver / Q000201:enzymology*; D008297:Male; D008780:Methyltransferases / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface
D000596:Amino Acids; D002352:Carrier Proteins; D058973:Folate Receptors, GPI-Anchored; D011956:Receptors, Cell Surface; D005492:Folic Acid; D008780:Methyltransferases; D050938:Glycine N-Methyltransferase; C494512:Gnmt protein, rat