Immuno-radiometric assays for human salivary acidic proline-rich proteins and their N- and C-terminal fragments.
An immuno-radiometric assay was developed for acidic proline-rich proteins from human saliva, and assays designed which specifically detect the N- or C-terminal parts of the proteins. The immuno-radiometric assay depends on the binding of antigen to paper discs coated with specific antibodies. Subsequently, the discs are incubated with radioactive specific antibodies. The amount of radioactive antibodies bound to the discs depends on the amount of antigen already adhering to the discs.
D006801:Humans; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D055232:Proline-Rich Protein Domains; D011863:Radioimmunoassay / Q000379:methods; D012463:Saliva / Q000032:analysis; D055273:Salivary Proline-Rich Proteins; D012471:Salivary Proteins and Peptides / Q000032:analysis*
C524536:PRH1 protein, human; D010446:Peptide Fragments; D010455:Peptides; D055273:Salivary Proline-Rich Proteins; D012471:Salivary Proteins and Peptides