Phenotypic changes induced by a novel benzophenone derivative and resultant suppression of cell proliferation in the human thymic acute lymphoblastic leukemia cell line HPB-ALL.
A novel benzophenone derivative, 2-(2-benzoyl-4-chlorophenoxy)-N-methylpropionamide (Ch-13), induced phenotypic differentiation linked to the inhibition of cell proliferation in human thymic acute lymphoblastic leukemia cell line HPB-ALL cells. The Ch-13-induced morphological changes consist of a decrease in cell size, nucleolar disappearance, and an alteration in the chromatin distribution, resembling large or atypical lymphocytes. Treatment with Ch-13 brought about a remarkable reduction in OKT6-, OKT4/Leu3a-, and OKT9-positive cells. At the optimal differentiation-inducing dose (5 X 10(-5)M), Ch-13 inhibited the cell proliferation, de novo DNA synthesis, and specific antibody-induced cell surface antigen capping.
D000954:Antigens, Surface / Q000032:analysis; D001577:Benzophenones / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006801:Humans; D007700:Kinetics; D007945:Leukemia, Lymphoid / Q000473:pathology*; D010641:Phenotype; D013329:Structure-Activity Relationship; D013953:Thymus Neoplasms / Q000473:pathology*
D000954:Antigens, Surface; D001577:Benzophenones; C043643:2-(2-benzoyl-4-chlorophenoxy)-N-methylpropionamide