Determination of free and total cholesterol in human bile samples using an enzyme electrode.
A reliable cholesterol electrode was assembled with a Clark type oxygen electrode and cholesterol oxidase immobilised on a nylon net fixed on the electrode surface. Determination of free (unesterified) and total (esterified and unesterified) cholesterol in human bile samples was performed. Results were compared with the enzymatic-spectrophotometric procedure based on the Roeschlau method. The electrode has a usable life of more than two weeks, and more than 300 determinations could be performed. The CV values for free and total cholesterol in bile samples is 5-6%. The comparison between the proposed procedure and the spectrophotometric procedure is fair for the half of the results (delta less than 5%) and acceptable in the other half (delta less than 25%).
D015096:3-Hydroxysteroid Dehydrogenases; D000328:Adult; D001646:Bile / Q000032:analysis*; D002784:Cholesterol / Q000032:analysis*; D002789:Cholesterol Oxidase; D004566:Electrodes; D004800:Enzymes, Immobilized; D005260:Female; D006801:Humans; D008297:Male; D008722:Methods; D008875:Middle Aged
D004800:Enzymes, Immobilized; D002784:Cholesterol; D015096:3-Hydroxysteroid Dehydrogenases; D002789:Cholesterol Oxidase