Stimulation of diploid fibroblast growth with serum-free medium conditioned by mezerein-treated monocytic leukemia cells.
Medium conditioned by mezerein-treated human acute monocytic leukemia cells (THP-1) stimulated human fibroblast replication. Maximum mitogenic activity was elaborated by THP-1 cells with a 24-hr incubation in 10(-7) M mezerein (activator phase) followed by a 36-hr incubation in insulin-supplemented serum-free Roswell Park Memorial Institute (RPMI)-1640 medium (effector phase). Growth stimulation was not due to the presence of residual mezerein. We previously reported that leukemia cells also produced a growth inhibitor. Fibroblast stimulation was resolved by isoelectrofocusing into several active fractions separate from the growth inhibitory activity for malignant mammary cells. Conditioned medium was mitogenic for fibroblasts in the presence of high concentrations of fetal bovine and human whole blood sera. Growth stimulation was observed in plasma-derived serum only when supplemented with exogenous platelet-derived growth factor. Thus, this THP-1 cell product does not fulfill the role of a competence factor.
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000503:physiopathology; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D004171:Diploidy; D004224:Diterpenes; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D006133:Growth Substances; D006801:Humans; D007700:Kinetics; D007948:Leukemia, Monocytic, Acute / Q000503:physiopathology*; D008168:Lung / Q000196:embryology; D010703:Phorbol Esters / Q000494:pharmacology*; D010704:Phorbols / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D013729:Terpenes
D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D006133:Growth Substances; D010703:Phorbol Esters; D010704:Phorbols; D010982:Platelet-Derived Growth Factor; D013729:Terpenes; C011309:mezerein