Induction of hapten-specific lymphoid cell proliferation by liposome-carrying molecules from haptenated epidermal cells in contact sensitivity.
Liposomes containing the extract of trinitrophenylated (TNP) epidermal cells stimulated lymph node cells from TNCB-sensitized mice effectively. Optimal stimulation was achieved by the liposome containing 0.06 microgram of the extract of TNP epidermal cells/microgram of phospholipid at a concentration of 20 micrograms phospholipid/ml. The response was hapten-specific and macrophage-dependent. UV irradiation of the epidermal cells abolished their ability to stimulate the lymph node cells. Furthermore, the liposomes containing the extract of TNP epidermal cells treated with anti-Ia and complement failed to stimulate the lymph node cells. The liposomes recovered their reactivity when nonhaptenated Ia antigen-rich epidermal extract was added. Taken together, it is likely that haptenated molecules and Ia antigen on the liposome stimulated T-lymphocytes in the presence of macrophages.
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D003877:Dermatitis, Contact / Q000209:etiology* / Q000276:immunology; D004817:Epidermis / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D006241:Haptens / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007114:Immunization / Q000379:methods; D008081:Liposomes / Q000276:immunology* / Q000528:radiation effects; D008198:Lymph Nodes / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D014301:Trinitrobenzenes / Q000276:immunology; D014466:Ultraviolet Rays
D006241:Haptens; D000949:Histocompatibility Antigens Class II; D008081:Liposomes; D014301:Trinitrobenzenes