Unexpected occurrence of the Ca 19-9 tumor marker in normal human seminal plasma.
A monoclonal antibody with anti-sialyl-lacto-N-fucopentaose II specificity, originally thought to be a specific tumor marker in pancreas- and gastrointestinal tumors, reacts strongly with fucose-rich glycoproteins from human seminal plasma obtained by phenol/saline extraction. They are presumed to come from the prostatic gland secretion. Because of the specificity of this monoclonal antibody it can be deduced that the sialyl-Lea-sequence: N-acetyl-alpha-neuraminyl-(2----3)-beta-D-galactopyranosyl-(1--- -3)-[alpha-L-fucopyranosyl-(1----4)]-2-acetamido-2-deoxy-beta-D-gluco pyranosyl-(1----3)-D-galactopyranosyl is present on these glycoproteins. This is also supported by our carbohydrate analysis.
D000951:Antigens, Neoplasm / Q000032:analysis*; D015295:Antigens, Tumor-Associated, Carbohydrate; D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D006868:Hydrolysis; D008297:Male; D009439:Neuraminidase / Q000378:metabolism; D011863:Radioimmunoassay / Q000379:methods; D012661:Semen / Q000032:analysis*
D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D009439:Neuraminidase