Phenotypic changes induced in the human thymic ALL cell line HPB-ALL by an ingenol ester, milliamin.
An ingenol ester, milliamin, induced phenotypic changes in human thymic ALL cell line HPB-ALL. These alterations included morphological changes, increased sRBC-rosetting, the induction of phenotypic changes of differentiation antigens, i.e. a decrease in OKT4-, OKT6-, OKT9-, OKT10- and Leu3a-positive cells and an increase in Leu2a-positive cells, growth inhibition and decreased DNA synthesis. These phenotypic changes were qualitatively and quantitatively similar to those induced by other polyol diterpens with tigliane and daphnane hydrocarbon skeletons such as TPA and mezereine, respectively.
D000954:Antigens, Surface / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D006801:Humans; D007945:Leukemia, Lymphoid / Q000473:pathology; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism; D012397:Rosette Formation; D013950:Thymus Gland / Q000166:cytology
D000954:Antigens, Surface; D004224:Diterpenes; D011064:Poly Adenosine Diphosphate Ribose; C039975:milliamine