Phosphorylation of rat heart ornithine decarboxylase by type-2 casein kinase.
Highly purified preparations of rat heart ornithine decarboxylase are readily phosphorylated by rat liver type-2 casein kinase-TS at the same 54 KDa protein band which is also radiolabeled by 3H-DFMO. The reaction, which is stimulated by polylysine leads to the incorporation of up to 0.8 mol P/mol ornithine decarboxylase at seryl residue(s) included in a single 8.6 KDa CNBr fragment. Partially purified preparations of ornithine decarboxylase contain a type-2 casein kinase which promotes the phosphorylation of ornithine decarboxylase at the same CNBr fragment affected by rat liver casein kinase-TS.
D000818:Animals; D047388:Casein Kinases; D006321:Heart / Q000187:drug effects; D007545:Isoproterenol / Q000494:pharmacology; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D009206:Myocardium / Q000201:enzymology*; D009955:Ornithine Decarboxylase / Q000302:isolation & purification / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats
D011494:Protein Kinases; D047388:Casein Kinases; D009955:Ornithine Decarboxylase; D007545:Isoproterenol