DNA sequence preference of the progesterone receptor.
Highly purified hen oviduct progesterone receptor A subunit was tested for binding to several chicken gene DNAs. Sequence preference detected by nitrocellulose filter adsorption of [32P]DNA fragments obtained from recombinant plasmids revealed a marked retention of certain DNA fragments. About a 10-fold preference was seen for DNA fragments flanking the 5' end of the steroid-regulated genes ovalbumin and gene Y. No preference was seen with analogous DNA fragments from chicken beta-globin and alpha-actin genes. Restriction endonuclease mapping suggests the presence of multiple receptor interaction sites flanking the 5' terminus of the ovalbumin gene. One of these preferential binding sites was localized between -135 and -247 base pairs upstream from the start of transcription. This region contains an 18-base-pair A + T-rich sequence, a likely candidate for the binding site itself, because earlier studies had shown receptor A to have marked preference for A + T-rich DNA.
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002645:Chickens; D004247:DNA / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D005796:Genes; D010047:Ovalbumin / Q000235:genetics; D011980:Receptors, Progesterone / Q000378:metabolism*
D011980:Receptors, Progesterone; D010047:Ovalbumin; D004247:DNA