An Ecteola-cellulose chromatography assay for 3'-phosphoadenosine 5'-phosphosulfate: phenol sulfotransferase.
A new assay procedure for phenol sulfotransferase which employs [35S]-3'-phosphoadenosine-5'-phosphosulfate as a sulfate donor and a variety of phenols as sulfate acceptors was developed. The appearance of the 35S-sulfated products or the disappearance of the [35S]-3'-phosphoadenosine-5'-phosphosulfate are determined simultaneously by chromatography of the assay incubation mixtures on Ecteola-cellulose columns, eluting with an NH4HCO3 step gradient. Various acidic, neutral, and basic phenols can be employed as substrates for phenol sulfotransferase using this procedure.
D000818:Animals; D015239:Arylsulfotransferase; D001921:Brain / Q000201:enzymology; D002845:Chromatography / Q000379:methods; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D051381:Rats; D013270:Stomach / Q000201:enzymology; D013379:Substrate Specificity; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000302:isolation & purification*
C022321:PAPS sulfotransferase; D013466:Sulfurtransferases; D015238:Sulfotransferases; D015239:Arylsulfotransferase