Peptide antibody specific for the amino terminus of skeletal muscle alpha-actin.
The NH2-terminal peptide of skeletal muscle alpha-actin (S alpha N peptide), which contains a primary sequence unique to this actin isozyme, was used to prepare an isozyme-specific peptide antibody. S alpha N peptide was purified from chicken breast muscle actin by preparative reverse-phase HPLC and was coupled to hemocyanin. This complex was used to immunize rabbits in order to elicit actin antibodies specific for the skeletal muscle alpha-actin isozyme. The antibody obtained, called S alpha N antibody, was reactive with S alpha N peptide and with skeletal muscle alpha-actin as well as with cardiac muscle alpha-actin. S alpha N antibody did not react with either of the actin isozymes present in smooth muscle (smooth muscle alpha and gamma) or in brain (nonmuscle beta and gamma). S alpha N antibody was used to detect muscle-specific actin in differentiating mouse and human myoblasts by using immunoblots of myoblast extracts and immunofluorescent staining of fixed cells.
D000199:Actins / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D003599:Cytoskeleton / Q000648:ultrastructure; D009132:Muscles / Q000196:embryology / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology
D000199:Actins; D010446:Peptide Fragments