Inhibition of 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) activity in the granulosa cells of mouse ovarian follicles by follicular fluid peptide.
A protein fraction (GF2) was purified from sheep ovarian follicular fluid. Its action on 3 beta-HSD activity in the mouse granulosa cells was measured in an in vitro system. The fraction (GF2) caused dose-dependent depletion of the 3 beta-HSD activity in granulosa cells and progesterone in the spent medium. A maximum inhibition of the activity was achieved after 30 min incubation of the granulosa cells with the GF2 fraction. Further purified HPLC fraction (Fr1) also inhibited 3 beta-HSD activity. In vivo administration of the GF2 fraction in normal cycling female mice also decreased the 3 beta-HSD activity in the granulosa cells of the ovarian follicles and plasma progesterone levels indicating the GF2 fraction to be a 3 beta-HSD inhibitor.
D015096:3-Hydroxysteroid Dehydrogenases / Q000037:antagonists & inhibitors*; D000818:Animals; D005260:Female; D006107:Granulosa Cells / Q000201:enzymology*; D006358:Hot Temperature; D007700:Kinetics; D051379:Mice; D006080:Ovarian Follicle / Q000502:physiology*; D011375:Progesterone Reductase / Q000037:antagonists & inhibitors*; D011506:Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D012756:Sheep; D014020:Tissue Extracts / Q000494:pharmacology*; D014357:Trypsin
D011506:Proteins; D014020:Tissue Extracts; D015096:3-Hydroxysteroid Dehydrogenases; D011375:Progesterone Reductase; D014357:Trypsin