Differentiation of myoblast cell lines and biological methylation: 3-deazaadenosine stimulates formation of multinucleated myofibers.
Treatment of myoblast cell lines with 3-deazaadenosine stimulates differentiation into myofibers. Myoblast clone L5/ 3B5 , which does not form myofibers after 6 days in fusion medium, was stimulated to form myofibers after 5 days of culture in fusion medium containing 50 microM 3-deazaadenosine. Myoblast clone L5/ 3C4 , which normally begins to form myofibers after 4 days in fusion medium, was stimulated by 50 microM 3-deazaadenosine to form myofibers after 3 days in culture and the extent of fusion was also increased. In the presence of 100 microM homocysteine thiolactone, the concentration of 3-deazaadenosine that stimulated maximal fusion was reduced by a factor of 10, from 50 microM to 5 microM 3-deazaadenosine. Stimulation of myofiber formation by 3-deazaadenosine suggests a requirement for one or more methylation reactions in myoblast differentiation and the potentiation by homocysteine thiolactone indicates that myofiber formation is specifically stimulated by an intracellular accumulation of 3-deazaadenosylhomocysteine.
D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells; D003402:Creatine Kinase / Q000378:metabolism; D004247:DNA / Q000032:analysis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007536:Isomerism; D009132:Muscles / Q000502:physiology*; D051381:Rats; D012263:Ribonucleosides / Q000494:pharmacology*; D014372:Tubercidin / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D012263:Ribonucleosides; C018258:3-deazaadenosine; C034583:3-deazaaristeromycin; D004247:DNA; D003402:Creatine Kinase; D000241:Adenosine; D014372:Tubercidin