Polysaccharide production by cell free transferases in saliva in relation to salivary microflora.
The supernatant of centrifuged whole saliva was incubated with radiolabeled sucrose to measure polysaccharide production by cell free transferases and to examine whether water-insoluble polysaccharides were produced. Amounts of polysaccharides were considered to reflect the level of cell free transferases in saliva. Plating samples on blood agar, MS and MSB plates gave salivary counts of total CFU, total streptococci, S. salivarius and S. mutans. The capability of the cell free portion of saliva to produce polymers was confirmed and it appeared that the cell free transferases were able to produce water-insoluble polysaccharides. Significant correlations were found between the total and the insoluble polysaccharides from 14C-G'-sucrose and total CFU, total streptococci, S. salivarius and S. mutans, respectively. Heavily S. mutans infected subjects seemed to produce particularly large amounts of water-insoluble polysaccharides from 14C-G'-sucrose. The apparently water-insoluble 14C-F'-polysaccharides correlated significantly to the number of S. salivarius. It was thus concluded that the constitution of the oral microflora and particularly the levels of S. mutans and S. salivarius were of importance for the level of cell free transferase activity.
D002474:Cell-Free System; D005609:Free Radicals; D006801:Humans; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000096:biosynthesis*; D012463:Saliva / Q000032:analysis / Q000201:enzymology / Q000382:microbiology*; D012995:Solubility; D013291:Streptococcus / Q000201:enzymology; D013295:Streptococcus mutans / Q000201:enzymology; D014166:Transferases / Q000378:metabolism*
D005609:Free Radicals; D011135:Polysaccharides, Bacterial; D014166:Transferases