Investigation of factors influencing 11 beta-hydroxysteroid dehydrogenase (EC 1.1.1.146) activity in midgestational human fetal lung monolayer and explant cultures.
We recently suggested that the 11 beta-hydroxysteroid dehydrogenase (11-HSD) activity in midgestational human fetal lung (HFL) cultures comprised at least two enzymes, one oxidative--associated with epithelial cells, the other reductive--related to fibroblast-like cells. In this study, the effects of various hormones on 11-HSD activity were studied by measuring the interconversion of [3H]cortisol and [14C]cortisone. Human chorionic gonadotrophin, placental medium, and low oxygen concentration increased the conversion of cortisone to cortisol while activity in the reverse direction remained unchanged. No effects were seen when adrenocorticotrophin, prolactin, placental lactogen, estrogens, triiodothyronine or oxytocin were added in physiological amounts.
D042842:11-beta-Hydroxysteroid Dehydrogenases; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003348:Cortisone / Q000378:metabolism; D046508:Culture Techniques; D005333:Fetus / Q000201:enzymology; D005865:Gestational Age; D006728:Hormones / Q000494:pharmacology; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D008168:Lung / Q000196:embryology / Q000201:enzymology*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000494:pharmacology; D010920:Placenta / Q000502:physiology; D013379:Substrate Specificity
D006063:Chorionic Gonadotropin; D006728:Hormones; D006913:Hydroxysteroid Dehydrogenases; D042842:11-beta-Hydroxysteroid Dehydrogenases; D010100:Oxygen; D003348:Cortisone; D006854:Hydrocortisone