Erythroid stem cells in Rauscher viral erythroleukemia.
Results reported herein describe the expansion of erythroid stem cells (CFU-e and BFU-e) from marrow and spleen cells from mice infected with Rauscher leukemia virus (RLV). Maximum colony numbers were seen by 5 weeks after viral inoculation. The increase in splenic CFU-e and BFU-e was related to development of splenomegaly, the latter being a characteristic feature of murine oncornavirus infection. Both splenic and marrow RLV-derived CFU-e were erythropoietin (Ep) dependent. However, splenic and marrow RLV-derived BFU-e proliferation occurred at Ep levels considered suboptimal for normal colony formation. This increased erythroid stem cell proliferation may be due in part to the increased demand on erythropoiesis as a result of the profound fatal anemia which occurs in RLV-induced erythroleukemia. These studies suggest that factors operating at the level of BFU-e may enhance erythropoiesis, and hence may be a direct result of murine oncornavirus infection.
D000328:Adult; D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D003114:Colony-Forming Units Assay; D004920:Erythropoiesis; D004921:Erythropoietin / Q000494:pharmacology; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology* / Q000503:physiopathology; D007942:Leukemia, Experimental / Q000473:pathology* / Q000503:physiopathology; D011925:Rauscher Virus; D013154:Spleen / Q000473:pathology
D004921:Erythropoietin