Crystallization and preliminary x-ray studies of spinach ribulose 1,5-bisphosphate carboxylase/oxygenase complexed with activator and a transition state analogue.
Ribulose 1,5-bisphosphate carboxylase/oxygenase has been purified from spinach and crystallized by equilibrium vapor diffusion with polyethylene glycol 6000 as a precipitant. Crystals suitable for x-ray studies were obtained from a binary complex with a transition state analogue, 2-C-carboxy-D-arabinitol 1,5-bisphosphate, and a quaternary complex with 2-C-carboxy-D-arabinitol 1,5-bisphosphate, Mg2+, and HCO-3. Two forms of crystals were obtained in the presence of 2-C-carboxy-D-arabinitol 1,5-bisphosphate. Form B crystals are plates which have orthorhombic space group P2(1)2(1)2 with unit cell dimensions a = 184 A, b = 218 A, and c = 119 A. Form C crystals are tetragonal needles with space group I422 and with cell dimensions a = b = 275 A and c = 178 A. In both forms, the asymmetric unit contains half a molecule.
D001639:Bicarbonates / Q000378:metabolism; D003460:Crystallization; D004789:Enzyme Activation; D008274:Magnesium / Q000378:metabolism; D010105:Oxygenases / Q000378:metabolism*; D010428:Pentosephosphates; D010944:Plants / Q000201:enzymology*; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism*; D013402:Sugar Alcohols; D013403:Sugar Phosphates / Q000378:metabolism; D014965:X-Rays
D001639:Bicarbonates; D010428:Pentosephosphates; D013402:Sugar Alcohols; D013403:Sugar Phosphates; C032687:2-carboxyarabinitol 1,5-biphosphate; D010105:Oxygenases; D012273:Ribulose-Bisphosphate Carboxylase; D008274:Magnesium