Prediction of sensitivity to 1-beta-D-arabinofuranosylcytosine by the plateau level of its 5'-triphosphate in human lymphoblastic cell lines in vitro.
Parameters for metabolism of 1-beta-D-arabinofuranosylcytosine (ara-C) were examined to know whether the prediction of ara-C sensitivity is possible or not using 9 human lymphoblastic cell lines, 3 T cell lines and 6 B cell lines in vitro. Neither the capacity for synthesis, degradation of 1-beta-D-arabinofuranosylcytosine 5'-triphosphate (ara-CTP), generally lower in the T than B cell lines, nor deamination of ara-C, negligible in the B cell lines, could be correlated to the drug sensitivity. On the other hand, significant correlation was obtained between the sensitivity and the plateau level of ara-CTP. We consider that ara-C sensitivity could be predicted by measuring the plateau ara-CTP level before commencement of chemotherapy.
D001085:Arabinofuranosylcytosine Triphosphate / Q000378:metabolism*; D001088:Arabinonucleotides / Q000378:metabolism*; D002051:Burkitt Lymphoma / Q000378:metabolism; D002460:Cell Line; D003561:Cytarabine / Q000378:metabolism* / Q000627:therapeutic use; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007945:Leukemia, Lymphoid / Q000188:drug therapy / Q000378:metabolism*
D001088:Arabinonucleotides; D003561:Cytarabine; D001085:Arabinofuranosylcytosine Triphosphate; D004247:DNA