Effects on fertilization of rabbits of insemination with ejaculates treated with PG-dehydrogenase and antisera to PGE-2 and PGF-2 alpha.
PGE-2 and PGF-2 alpha in rabbit semen were selectively inactivated by incubation with antisera, or most of the seminal prostaglandins were transformed into biologically inactive 15-keto-prostaglandins by prostaglandin-15-hydroxydehydrogenase(PG-15-HDH). These treated ejaculates were vaginally inseminated. Compared to the controls (738 eggs of which 94% were fertilized) a dose-dependent reduction of the fertilization rate was observed with the anti-PGF-2 alpha-treated ejaculates. A non-uniformly, but statistically significantly reduced fertility was found in the other 2 treatment groups. After incubation with higher doses of PG-15-HDH, some fertilization was accomplished with ejaculates showing an extremely weak forward progression or immotile spermatozoa. An improvement in sperm motility, however, was observed in ejaculates treated with antiserum to PGF-2 alpha. Seminal prostaglandins may not exclusively affect sperm motility. The observed influences on the fertilization rate after treatment of spermatozoa with antisera to PGE-2 and PGF-2 alpha or PG-15-HDH suggest that these are local effects in the female genital tract.
D000818:Animals; D015237:Dinoprost; D015232:Dinoprostone; D005260:Female; D005306:Fertilization / Q000187:drug effects; D006911:Hydroxyprostaglandin Dehydrogenases / Q000494:pharmacology*; D007106:Immune Sera / Q000494:pharmacology; D007315:Insemination, Artificial; D008297:Male; D011458:Prostaglandins E / Q000502:physiology*; D011460:Prostaglandins F / Q000502:physiology*; D011817:Rabbits; D012661:Semen / Q000187:drug effects / Q000378:metabolism*
D007106:Immune Sera; D011458:Prostaglandins E; D011460:Prostaglandins F; D015237:Dinoprost; D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase; D015232:Dinoprostone