Nuclear matrix DNA from chicken erythrocytes contains beta-globin gene sequences.
Nuclear matrices containing residual DNA were isolated from chicken erythrocytes after extraction of purified nuclei with buffered 2 M NaCl. After further purification of this residual DNA, it was found to contain high concentrations of beta-globin gene sequences as assayed by dot hybridization with 32P-labeled nick-translated pHB1001. Electron microscopy of a random sample of this residual DNA fraction shows the DNA to be intimately associated with protein at various intervals. A hypothesis for enrichment of active genes in residual DNA from purified chromatin or in nuclear matrix DNA is also discussed.
D000818:Animals; D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure*; D002645:Chickens; D004247:DNA / Q000378:metabolism; D004912:Erythrocytes / Q000502:physiology; D005786:Gene Expression Regulation; D005796:Genes; D005914:Globins / Q000235:genetics*; D011485:Protein Binding; D012995:Solubility
D005914:Globins; D004247:DNA