Glutathione S-transferase activity in rat pancreas.
The biotransformation capability of pancreatic tissue was studied. Michaelis-Menten kinetics were determined for glutathione S-transferase in pancreatic tissue supernatants from control, phenobarbital (PB), and 3-methylcholanthrene (3MCA)-pretreated Sprague-Dawley male rats. The enzyme activity was measured spectrophotometrically with use of 1-chloro-2,4-dinitrobenzene as substrate. Pancreatic activity was about half the hepatic activity, and, further, the activity was not affected by pretreatment with PB or 3MCA as was the liver.
D000818:Animals; D001711:Biotransformation; D004790:Enzyme Induction / Q000187:drug effects; D005982:Glutathione Transferase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008748:Methylcholanthrene / Q000494:pharmacology; D010179:Pancreas / Q000201:enzymology*; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D008748:Methylcholanthrene; D005982:Glutathione Transferase; D010634:Phenobarbital