Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus.
Thirty-nine new temperature-sensitive mutants of vaccinia virus have been isolated, expanding a previously reported collection of mutants (R. C. Condit and A. Motyczka, Virology 113, 224-241, 1981) to a total of 65. The 65 mutants have been assigned to 32 complementation groups, based primarily on a qualitative spot test described previously (Condit and Motyczka, 1981). Representatives of each complementation group have been assayed for DNA and protein synthesis at the nonpermissive temperature, revealing one new DNA-negative complementation group, three new groups which contain mutants defective in late protein synthesis, and ten new groups containing mutants which synthesize DNA and protein in a normal fashion. Marker rescue has been achieved with 29 of the 65 mutants using cloned DNA fragments from wild-type virus. These 29 mutants together represent 20 of the 32 complementation groups. A preliminary physical map of the mutants is presented.
D004279:DNA, Viral / Q000096:biosynthesis; D005814:Genes, Viral; D005816:Genetic Complementation Test; D005819:Genetic Markers; D009154:Mutation; D013696:Temperature; D014616:Vaccinia virus / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis
D004279:DNA, Viral; D005819:Genetic Markers; D014764:Viral Proteins