Differences in basic proline-rich proteins in rat parotid saliva following chronic isoproterenol treatment or maintenance on a liquid diet.
The basic proline-rich proteins (BPRP) in the stimulated parotid saliva of rats treated for 8 days with isoproterenol and rats fed a liquid diet for 2 weeks were compared to those in the stimulated parotid saliva of untreated rats fed a stock pelleted-diet (control). In the control, the BPRP were separated into 5 groups designated Peak A (the basic proline-rich glycoprotein), SP-1, SP-2, SP-3 and SP-4. The percentage of BPRP in each group was as follows: Peak A, 6.5 per cent; SP-1, 37 per cent; SP-2, 6.5 per cent; SP-3, 32.4 per cent; SP-4, 17.6 per cent. In the parotid saliva of rats fed the liquid diet, proteins corresponding to Peak A and SP-2 were not present, the proportion of BPRP in SP-4 was increased to almost 90 per cent while the proportions of material in SP-1 and SP-3 were reduced to 3 and 8 per cent, respectively. In the saliva of rats subjected to chronic isoproterenol treatment, a protein corresponding to SP-4 was not present; proteins corresponding to Peak A, SP-1 and SP-3 were present and in amounts similar to their proportion in untreated rats although material in SP-2 increased to 36 per cent.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002852:Chromatography, Ion Exchange; D004032:Diet; D004591:Electrophoresis, Polyacrylamide Gel; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D010306:Parotid Gland / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000032:analysis*; D011392:Proline / Q000032:analysis*; D055232:Proline-Rich Protein Domains; D051381:Rats; D011919:Rats, Inbred Strains; D012463:Saliva / Q000032:analysis* / Q000187:drug effects; D012471:Salivary Proteins and Peptides / Q000032:analysis*
D000596:Amino Acids; D010455:Peptides; D012471:Salivary Proteins and Peptides; D011392:Proline; D007545:Isoproterenol