Chemosensitivity testing of nonsolid tumors by the subrenal-capsule implant assay.
A procedure to test chemosensitivity of nonsolid tumors using the subrenal-capsule xenograft assay has been developed. This technique is applicable to the study of malignant effusions and hematopoietic tumors. The tumor cells are centrifuged to form a pellet and the pellet is resuspended in the presence of bovine fibrinogen to form a jelled clot. This clot is cut into fragments which are implanted beneath the renal capsule of normal mice. The growth of the tumor cells from ovarian adenocarcinoma obtained from ascitic fluid is better than that of tumor fragments from the solid tumors. This is probably a reflection of the greater viability of tumor cells from ascitic fluids. The sensitivity to chemotherapy was the same for the solid tumor and its malignant effusions.
D000230:Adenocarcinoma / Q000188:drug therapy / Q000473:pathology; D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D001201:Ascites / Q000473:pathology; D002945:Cisplatin / Q000008:administration & dosage; D003114:Colony-Forming Units Assay; D003520:Cyclophosphamide / Q000008:administration & dosage; D004317:Doxorubicin / Q000008:administration & dosage; D005260:Female; D006801:Humans; D007951:Leukemia, Myeloid / Q000473:pathology; D051379:Mice; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000473:pathology; D014183:Transplantation, Heterologous; D014410:Tumor Stem Cell Assay
D004317:Doxorubicin; D003520:Cyclophosphamide; D002945:Cisplatin