Expression of MY7 antigen on myeloid precursor cells.
A murine monoclonal antibody (anti-MY7) has been developed that detects an antigen expressed by 6% of normal human bone marrow cells, including approximately 40% of myeloid colony-forming cells (CFU-C). The number of bone marrow cells and CFU-C expressing MY7 is significantly increased in regenerating bone marrow, but less than 5% of peripheral blood CFU-C express the MY7 antigen. Erythroid precursors are MY7 negative from peripheral blood and bone marrow. Thymidine suicide studies indicate that CFU-C in S-phase tend to be MY7 positive while CFU-C not in S-phase are MY7 negative. MY7 expression thus appears to identify a fraction of CFU-C that is actively proliferating.
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D001853:Bone Marrow / Q000502:physiology; D001854:Bone Marrow Cells; D002453:Cell Cycle; D004247:DNA / Q000096:biosynthesis; D004912:Erythrocytes / Q000276:immunology; D006098:Granulocytes / Q000276:immunology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007945:Leukemia, Lymphoid / Q000097:blood; D009000:Monocytes / Q000276:immunology*; D012038:Regeneration
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D004247:DNA