A sensitive and dependable assay for distinguishing hamster and human X-linked steroid sulfatase activity in somatic cell hybrids.
A radioisotopic assay is described to distinguish between Chinese hamster and human steroid sulfatase activity in extracts prepared from hamster X human somatic cell hybrids. This assay is based on different pH optima and provides a sensitive and unambiguous biochemical marker for the short arm of the human X chromosome and as well as for otherwise genetically inactivated X chromosomes in rodent X human hybrids.
D000818:Animals; D002874:Chromosome Mapping; D006224:Cricetinae; D005260:Female; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D008297:Male; D008722:Methods; D051379:Mice; D012867:Skin / Q000166:cytology; D013045:Species Specificity; D043266:Steryl-Sulfatase; D013429:Sulfatases / Q000032:analysis / Q000235:genetics*; D014960:X Chromosome
D013429:Sulfatases; D043266:Steryl-Sulfatase