Transformation of human cells by DNA transfection.
Normal human cells can be transformed to anchorage-independent growth by transfection with DNA from MOLT-4 lymphoblasts, derived from a patient with acute lymphocytic leukemia. Cells were treated with polyethylene glycol 6000, and DNA was administered as a calcium phosphate coprecipitate . The transforming activity of MOLT DNA was inactivated by treatment with DNase or by the restriction enzymes Bgll or BamHl but not by EcoRV . DNA from transfected colonies can transform human recipient cells to anchorage-independent growth; the transforming ability and anchorage independence are maintained stably for at least 30 generations.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics*; D005347:Fibroblasts; D005399:Fishes; D006801:Humans; D007231:Infant, Newborn; D007945:Leukemia, Lymphoid / Q000235:genetics; D008297:Male; D009369:Neoplasms / Q000235:genetics; D012867:Skin; D013094:Spermatozoa; D014162:Transfection
D004273:DNA, Neoplasm; D004247:DNA