Immunostimulatory mouse granuloma protein.
Earlier studies have shown that from subcutaneous talc-induced granuloma in mice, a fraction could be extracted that fully protected mice against Listeria monocytogenes. Using standard biochemical procedures--i.e., ammonium sulfate fractionation, preparative electrophoresis, gel filtration chromatography, isoelectric focusing, and preparative polyacrylamide gel electrophoresis--we have now purified an active factor to homogeneity. A single band was obtained in NaDodSO4/polyacrylamide gel with an apparent Mr of 55,000. It migrated with alpha 1-globulins and the isoelectric point was 5 +/- 0.1. The biological activity was destroyed with Pronase but not with trypsin and a monospecific polyclonal rabbit antiserum was obtained. The intravenous injection of 5 micrograms of this "mouse granuloma protein" fully protects mice against a lethal inoculum of L. monocytogenes. Moreover, after their incubation with 10 nM mouse granuloma protein, mouse peritoneal cells became cytostatic against Lewis carcinoma cells.
D000818:Animals; D006099:Granuloma / Q000276:immunology*; D007111:Immunity, Cellular; D007526:Isoelectric Point; D008088:Listeriosis / Q000276:immunology / Q000517:prevention & control; D008099:Liver / Q000276:immunology; D008222:Lymphokines / Q000276:immunology* / Q000302:isolation & purification; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000517:prevention & control; D009895:Opsonin Proteins / Q000276:immunology; D011506:Proteins / Q000276:immunology* / Q000302:isolation & purification; D013154:Spleen / Q000276:immunology
D008222:Lymphokines; D009895:Opsonin Proteins; D011506:Proteins; C039391:granuloma protein, mouse