New light on the Müller cell.
The fluorescent phenanthridinium dye, ethidium bromide, selectively penetrates Müller cells. Since the dye has a marked affinity for nucleic acid, the nucleus stands out brilliantly when retinal tissue sections are viewed in the fluorescence microscope. The monoazide derivative of ethidium bromide is known to have nucleic acid binding properties similar to those of the parent compound. On this basis a plan for photoaffinity labelling is put forward. Its relationship to the problem of the genesis of the electroretinogram is discussed.
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D004396:Coloring Agents; D004996:Ethidium; D008856:Microscopy, Fluorescence; D009457:Neuroglia / Q000648:ultrastructure*; D011898:Ranidae; D012160:Retina / Q000648:ultrastructure*
D004396:Coloring Agents; D004996:Ethidium