[Isolation of mononuclear cells from the bone marrow of patients with hemoblastoses using one-step ficoll-verographin density gradient separation].
The one-step ficoll-verographin density-gradient centrifugation was first used for isolating mononuclear cells from human bone marrow. Children suffering from various hemoblastoses were donors. The morphological control of the cells isolated from the interface layer and May-Grünwald stained showed a high efficiency of the procedure proposed. Due to the nosological form of malignancy mononuclears composed from 100% (in the relapse of acute lymphocytic leukemia) to 81% (in hemoblastosis with "intact" bone marrow: lymphogranulomatosis and lymphosarcoma without leukemization) among the isolated cells. Thus, the one-step ficoll-verographin density-gradient centrifugation may be used for extensive clinical research, permitting more time-consuming procedures to be avoided.
D000293:Adolescent; D001853:Bone Marrow / Q000473:pathology*; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D003974:Diatrizoate Meglumine; D005362:Ficoll; D006689:Hodgkin Disease / Q000473:pathology; D006801:Humans; D007945:Leukemia, Lymphoid / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000473:pathology*; D009000:Monocytes / Q000473:pathology*
D005362:Ficoll; D003974:Diatrizoate Meglumine
Vydelenie mononuklearnykh kletok iz kostnogo mozga bol'nykh gemoblastozami putem razdelenia v odnostupenchatom gradiente plotnosti fikoll-verografin.