Changes in the surface marker pattern related to maturation in adult acute myeloid leukaemia.
Introduction of the maturation index (MI) as a measure for the degree of maturation improved the subtyping of acute myeloid leukemia (AML). A comparison is made here between the MI and the results of surface marker analysis with a panel of monoclonal antibodies (McAb) in the immunofluorescence technique. The McAb applied in 46 AML patients (greater than or equal to 15 years) were granulocyte specific (MI/N1, UJ 308, B4-3, B13-9), granulocyte-monocyte specific (OKM-1, B2-12) or had specificity for the Ia-like antigen (OKI-1), 'T-cells' (3A1), immature cells (OKT-10) or platelets (C17-28). In 32 of these patients more McAb could be investigated with specificities for granulocytes (VIM-D5), granulocytes-monocytes (RUPI-5), monocytes (MONO BRL, RUPI-4), erythrocytes (VIE-G4) and AML cells (VIM-S8). An increase in surface marker expression evident from the reaction with a number of McAb (UJ 308, B2-12, OKM-1 and OKI-1) paralleled the rise of the MI in FAB M5. A decrease of the expression of antigen detected by OKI-1 paralleled the rise of the MI in FAB M1-3. The granulocyte or monocyte specific McAb, as they are determined on normal human peripheral blood cells, did not distinguish between FAB M1-3 and M5. The maturation index seems to be a valuable tool in understanding the results of surface marker analysis.
D000328:Adult; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000954:Antigens, Surface / Q000032:analysis*; D004912:Erythrocytes / Q000276:immunology; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000276:immunology* / Q000503:physiopathology; D009000:Monocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000954:Antigens, Surface