Amiloride potentiation of differentiation of human promyelocytic cell line HL-60.
Cells of the human acute promyelocytic cell line HL-60 undergo differentiation when exposed to dimethyl sulfoxide (DMSO); in this report, amiloride, an inhibitor of passive intracellular Na+ flux, potentiated the DMSO-induced differentiation of HL-60 cells. This effect was seen at several concentrations of DMSO. Amiloride alone did not affect HL-60 differentiation. Various analogues of amiloride were tested for their ability to potentiate differentiation of HL-60 cells. The synergistic induction of myeloid differentiation by a membrane solvent (DMSO) and an Na+ transport inhibitor (amiloride) suggested membrane cation flux as being important in initiating differentiation.
D000584:Amiloride / Q000031:analogs & derivatives / Q000494:pharmacology*; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004357:Drug Synergism; D006801:Humans; D066298:In Vitro Techniques; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D011719:Pyrazines / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D011719:Pyrazines; D000584:Amiloride; D004121:Dimethyl Sulfoxide