Extremely decreased release of prostaglandin E2-like activity from chopped lung of ethyl linolenate-supplemented rats.
Three groups of weanling male rats were reared on a fat-free diet for 13 weeks. One group received only the fat-free diet (FF rats), the other 2 groups received the fat-free diet and a daily supplement of 2 energy% ethyl linoleate ([n-6] rats), or 2 energy% ethyl linolenate ([n-3] rats). The chopped lung preparation was used to illustrate an in vitro prostaglandin formation. PGE2-like activity was quantified on rat stomach strip. The release of PGE2-like activity expressed as ng PGE2-equivalent per g lung tissue (mean +/- SD) was 23 +/- 7, less than 6, and 65 +/- 20 for the FF rats, the (n-3) rats, and the (n-6) rats, respectively. PGE2 quantification by radioimmunoassay of the chopped lung effluent collected after passing over the rat stomach strip revealed the same release pattern as the bioassay. Fractionation of chopped lung effluent on HPLC with radioimmunoassay detection indicated that the lung tissue from (n-3) rats released very little PGE3, if any, in spite of a 20:5(n-3)/20:4(n-6) ratio of 5.2 in the lipids of the lung. It is suggested that the pool of arachidonic acid for prostaglandin production in vitro is different from the one which functions in vivo, and the these pools are differently affected by dietary EFA.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D015232:Dinoprostone; D004305:Dose-Response Relationship, Drug; D008042:Linolenic Acids / Q000494:pharmacology*; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011458:Prostaglandins E / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D008042:Linolenic Acids; D011458:Prostaglandins E; D015232:Dinoprostone; C039896:ethyl linolenate