Isolation and purification of plasmids from Bacteroides fragilis using rubidium trichloroacetate density gradient centrifugation.
A rapid and easy final purification method is described for the isolation of plasmids from B. fragilis. Using RbTCA density gradient centrifugation in an airfuge ultracentrifuge ccc plasmid DNA can be separated from RNA, residual chromosomal DNA, linear and oc plasmid DNA. Pure ccc plasmid DNA is obtained from cultures of between 1 ml and 2 l in less than one day.
D001441:Bacteroides fragilis / Q000235:genetics*; D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000302:isolation & purification*; D010957:Plasmids; D012413:Rubidium; D014238:Trichloroacetic Acid
D004269:DNA, Bacterial; D014238:Trichloroacetic Acid; D012413:Rubidium