Relaxation of rabbit aorta by chlordimeform.
Chlordimeform N'-(4-choro-O-tolyl)-N,N-dimethyl-formamidine; CDF), an acaracide-insecticide, relaxed helically cut strips of rabbit thoracic aorta and deadventitiated aorta strips. Relaxations by CDF of aorta strips contracted by 5 x 10(-7) M prostaglandin F2 alpha were not altered by the antimuscarinic agent atropine (10(-5) M), the beta-adrenergic antagonist propranolol (10(-5) M), the histamine H2 antagonist cimetidine (2 x 10(-4) M), the dopamine antagonist haloperidol (10(-5) M) or the Na+-K+-ATPase inhibitor ouabain (5 x 10(-6) M). CDF further relaxed deadventitiated strips contracted by 10(-6) M NE or 40 mM potassium and then partially relaxed by 10(-5) M verapamil. CDF (10(-5) M) potentiated contractions caused by the addition of calcium to potassium depolarized strips in zero calcium media, but higher concentrations reduced calcium-induced contractions. The highest CDF concentration (10(-2) M) not only prevented tension increase on calcium addition, but also progressively decreased tension to below baseline levels. The addition of 10(-2) M CDF to aorta strips not contracted by a vasoactive agent decreased resting tension. This relaxation was usually maximal at 10(-3) M and less at 10(-2) M. Thus, CDF relaxes vascular smooth muscle, not by antagonism at the usual vascular relaxant receptors, but by interference with calcium utilization.
D000578:Amidines / Q000494:pharmacology*; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology; D002742:Chlorphenamidine / Q000494:pharmacology*; D015237:Dinoprost; D004347:Drug Interactions; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects*; D009126:Muscle Relaxation / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D011188:Potassium / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D011817:Rabbits; D014700:Verapamil / Q000494:pharmacology
D000578:Amidines; D011460:Prostaglandins F; D015237:Dinoprost; D014700:Verapamil; D002742:Chlorphenamidine; D011188:Potassium; D002118:Calcium