A single step method for the separation of rat liver cytosolic glutathione S-transferase isozymes.
A simple, single step chromatographic method was developed to separate the liver cytosolic glutathione S-transferase (GSH-S-t) isozymes from each other and from the bulk of the cytosolic protein. Five peaks of GSH-S-t activity, tested with 1-chloro-2,4-dinitrobenzene (CDNB) as a substrate, were eluted. By comparison of the activities with CDNB and the other substrate 3,4-dichloronitrobenzene (DCNB) the five peaks could be identified as GSH-S-t isozymes C, B, A, and AA, being GSH-S-t isozyme C eluted in two different peaks. The method was used to detect a decrease of specific GSH-S-t isozymes in the cytosol of rats intoxicated with carbon tetrachloride, as compared with control rats.
D000818:Animals; D002852:Chromatography, Ion Exchange; D003600:Cytosol / Q000201:enzymology*; D005982:Glutathione Transferase / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D007527:Isoenzymes; D005982:Glutathione Transferase