Simple method for preparing and quantifying very-low-density lipoprotein.
A micromethod is described for isolating and quantifying human very-low-density lipoprotein (VLDL) from 0.1 mL of human serum, by which more than 100 samples can be processed in half a day with a standard ultracentrifuge. Replicate analysis of 10 normal and hyperlipemic samples gave median coefficients of variation of 11.7% for VLDL cholesterol, 5.1% for VLDL triglycerides. Results obtained by micro-ultracentrifugation and enzymic cholesterol measurement agreed closely with those measured with an electrophoretic method (Pearson's r = 0.98). Compared with the established method, on the other hand, lower values were found for both VLDL cholesterol (r = 0.89) and triglycerides (r = 0.97).
D002784:Cholesterol / Q000302:isolation & purification; D015243:Cholesterol, VLDL; D004587:Electrophoresis, Agar Gel; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood*; D008079:Lipoproteins, VLDL / Q000302:isolation & purification*; D014280:Triglycerides / Q000302:isolation & purification
D015243:Cholesterol, VLDL; D008079:Lipoproteins, VLDL; D014280:Triglycerides; C030106:very low density lipoprotein triglyceride; D002784:Cholesterol