Codon specificity of starvation induced misreading.
Mistranslated derivatives of the coat protein of the bacteriophage MS2 were isolated from infected cells starved for asparagine. This protein contains a high level of lysine for asparagine substitutions. By peptide analysis and amino acid sequencing we show that there is a six-fold greater frequency of errors at AAU codons than at AAC codons. This ratio is the same as that found in unstarved cells where the overall error frequency is 100-fold less. We also demonstrate that, at least for AAC codons, context affects error frequency.
D000595:Amino Acid Sequence; D001216:Asparagine / Q000235:genetics* / Q000378:metabolism; D002213:Capsid / Q000096:biosynthesis* / Q000235:genetics; D036022:Capsid Proteins; D003062:Codon; D003090:Coliphages / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger; D016601:RNA-Binding Proteins
D036022:Capsid Proteins; D003062:Codon; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D001216:Asparagine