Isolated intestinal cells of guinea pig: a suitable model for assessing direct toxic effects of ethanol on the mucosa of the upper small intestine.
The effects of acute ethanol (1-10%) on membrane transport and respiration in isolated jejunal cells of guinea pig were investigated. Three substrates with different transport mechanisms were employed: uridine (active, Na+-dependent transport), choline (saturable, Na+-independent transport), and chenodeoxycholate (simple diffusion). Uridine and choline transport were half-maximally inhibited by 3% ethanol; this inhibition was paralleled by a similar decrease in mitochondrial respiration and ATP production. Chenodeoxycholate uptake in contrast was virtually unaffected by up to 10% ethanol. It is concluded that acute ethanol inhibits active carrier-mediated absorption, but has no effect on transcellular diffusion of lipophilic compounds.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002635:Chenodeoxycholic Acid / Q000378:metabolism; D002794:Choline / Q000378:metabolism; D000431:Ethanol / Q000633:toxicity*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects*; D007421:Intestine, Small / Q000187:drug effects; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D014529:Uridine / Q000378:metabolism
D002635:Chenodeoxycholic Acid; D000431:Ethanol; D000255:Adenosine Triphosphate; D002794:Choline; D014529:Uridine