Chromosomal instability in Streptomyces glaucescens: mapping of streptomycin-sensitive mutants.
Streptomyces glaucescens strain GLAO (=ETH 22794) produces hydroxystreptomycin and has a high natural resistance to hydroxystreptomycin, dihydrostreptomycin and streptomycin. The wild-type strain gives rise spontaneously to streptomycin-sensitive (StrS-) variants at a frequency of 0 . 2 to 1 . 4%. These mutants lack streptomycin phosphotransferase activity responsible for the wild-type resistance to streptomycin group antibiotics and are unable to produce detectable amounts of hydroxystreptomycin. Mapping experiments showed that the strS marker lies between the chromosomal markers lys-2 and ura-3 on the linkage map of S. glaucescens. The molecular basis for instability of this marker is as yet unknown.
D000483:Alleles; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003433:Crosses, Genetic; D004352:Drug Resistance, Microbial; D008040:Genetic Linkage; D005819:Genetic Markers; D009154:Mutation; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics*; D013307:Streptomycin / Q000494:pharmacology*
D005819:Genetic Markers; D013307:Streptomycin