Separation of Fe+3 from transferrin in endocytosis. Role of the acidic endosome.
NH4Cl and monensin, two agents which neutralize intracellular acidic compartments, block the segregation of iron from transferrin after endocytosis, while neither of these reagents affects internalization of diferric transferrin into the cell. In conclusion the molecular separation of iron from transferrin inside the cell requires a non-lysosomal acidic compartment.
D000643:Ammonium Chloride / Q000494:pharmacology; D002460:Cell Line; D004705:Endocytosis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism*; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute; D008830:Microbodies / Q000187:drug effects / Q000378:metabolism*; D008985:Monensin / Q000494:pharmacology; D009940:Organoids / Q000378:metabolism*; D011485:Protein Binding; D014168:Transferrin / Q000378:metabolism*
D014168:Transferrin; D000643:Ammonium Chloride; D008985:Monensin; D007501:Iron