Enzymatic modification of the lymphocyte surface. Application to tissue typing and rosetting with sheep erythrocytes.
Lymphocytes were treated with hydrolytic enzymes primarily to assess whether such modified cells would give improved cytotoxicity reactions during tissue typing. Papain-treated and alpha-chymotrypsin-treated lymphocytes were approximately twice as sensitive as untreated cells in the microcytotoxicity test used, and this finding might be usefully exploited by immunological laboratories for purposes of cross-matching, HLA antibody screening and HLA-DR typing. Trypsin treatment promoted massive cell clumping, while neuraminidase treatment was responsible for indiscriminate cell death after exposure to rabbit serum. The capacity of lymphocytes to form rosettes with sheep erythrocytes was abolished after treatment with trypsin or alpha-chymotrypsin, but enhanced by papain or neuraminidase.
D000818:Animals; D004912:Erythrocytes / Q000276:immunology; D006680:HLA Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D006650:Histocompatibility Testing; D006801:Humans; D006867:Hydrolases / Q000494:pharmacology*; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D011971:Receptors, Immunologic / Q000187:drug effects; D012397:Rosette Formation; D012756:Sheep
D006680:HLA Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D011971:Receptors, Immunologic; D006867:Hydrolases