Identification of a cDNA clone for mouse apoprotein A-1 (apo A-1) and its use in characterization of apo A-1 mRNA expression in liver and small intestine.
A cDNA clone for mouse apoprotein A-1 (apo A-1), the major apoprotein of plasma high density lipoproteins, has been identified. In addition to structural and physiological evidence, a genetic polymorphism for mouse plasma apo A-1 has been used to confirm that this DNA sequence corresponds to the apo A-1 gene. Use of this clone in molecular hybridization studies has shown that the concentration of apo A-1 mRNA is similar in liver and small intestine and is constant along the entire length of the small intestine. We provide evidence that the same apo A-1 gene is expressed in both liver and small intestine. Apo A-1 mRNA is also present in the stomach and esophagus at 10-15% the concentration found in small intestine but is undetectable in other tissues (such as large intestine, pancreas, heart, kidney, spleen, and brain). Finally, we show that there is a differential effect of a diet high in saturated fat and cholesterol on apo A-1 mRNA levels in liver and small intestine.
D000818:Animals; D001053:Apolipoproteins / Q000235:genetics*; D001054:Apolipoproteins A; D002784:Cholesterol / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004041:Dietary Fats / Q000378:metabolism; D005786:Gene Expression Regulation; D007421:Intestine, Small / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000235:genetics*
D001053:Apolipoproteins; D001054:Apolipoproteins A; D004041:Dietary Fats; D012333:RNA, Messenger; D004247:DNA; D002784:Cholesterol