Comparison in vitro of the incorporation of D-[2-3H(N)]-mannose and D-[1-14C]-glucosamine into glycoproteins of dispersed rat submandibular salivary gland cells.
The incorporation of two different radiolabelled sugars, D-[14C]-glucosamine and D-[2-3H(N)]-mannose, into cellular and secretory glycoproteins was compared using dispersed rat submandibular cells. Most of the de-novo biosynthesis appeared to be directed toward the synthesis of secretory material as the molecular profile of the 3H-labelled material released following sympathomimetic stimulation and the percentage of total 3H-labelled acid--precipitable material secreted following cholinergic- or adrenergic-receptor stimulation coincided with the data obtained from similar studies using [14C]-glucosamine. The [3H]-mannose label was found in the neutral sugars mannose, galactose, glucose and fucose, with trace amounts of radiolabel in the amino sugars, whereas the [14C]-glucosamine label was present in three different amino sugars; glucosamine, galactosamine and sialic acid.
D000818:Animals; D002469:Cell Separation; D005944:Glucosamine / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008297:Male; D008358:Mannose / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013363:Submandibular Gland / Q000166:cytology / Q000378:metabolism*
D006023:Glycoproteins; D005944:Glucosamine; D008358:Mannose