Electroanatomy of a unique amacrine cell in the rabbit retina.
Intracellular electrophysiological recordings were obtained from a specialized class of "starburst" amacrine cells by using an isolated superperfused retina-eyecup preparation of the rabbit. These cells were injected intracellularly with horseradish peroxidase and identified with light microscopy. A computer-controlled image-processing system was used to map and display the three-dimensional dendritic organization and provide information on length and sublaminar distribution of dendritic processes. Starburst amacrines show an unusual dendritic architecture that includes thin intermediate dendritic segments. Analysis with steady-state cable equations suggests that these thin segments may provide electrical isolation of distal processes, raising the possibility that a single dendrite, which lies beyond the thin segment, may constitute a functional subunit of the cell.
D000200:Action Potentials; D000818:Animals; D006735:Horseradish Peroxidase; D066298:In Vitro Techniques; D009431:Neural Conduction; D011817:Rabbits; D012160:Retina / Q000166:cytology* / Q000502:physiology*
D006735:Horseradish Peroxidase