Energy depletion retards the loss of membrane transport during reticulocyte maturation.
The effect of metabolic depletion on the maturation-associated loss of membrane functions has been studied by using sheep reticulocytes incubated in vitro at 37 degrees C for periods up to 41 hr. ATP was either maintained with glucose, adenosine plus inosine, or depleted with 2-deoxyglucose plus arsenate. Two membrane transport systems were studied: Na+-dependent glycine transport activity and the sodium pump, estimated from measurements of the number of [3H]ouabain binding sites per cell. Both transport systems were decreased during maturation. However, the decrease was much less in ATP-depleted cells compared to ATP-replete cells. It is concluded that the loss of certain functions during reticulocyte maturation is retarded by metabolic depletion.
D000255:Adenosine Triphosphate / Q000097:blood*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D004905:Erythrocyte Aging; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005998:Glycine / Q000097:blood*; D010042:Ouabain / Q000097:blood; D011955:Receptors, Drug / Q000378:metabolism; D012156:Reticulocytes / Q000378:metabolism*; D012756:Sheep; D012964:Sodium / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase
D011955:Receptors, Drug; C028996:cardiac glycoside receptors; D010042:Ouabain; D000255:Adenosine Triphosphate; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D005998:Glycine