Characterization of reiterated human DNA with respect to mammalian X chromosome homology.
Recombinants containing human repetitive DNA sequences were identified by dot hybridization and classified with respect to presence on the X chromosome and homology to mouse DNA. Using genomic probes that differ in number of X chromosomes, we observed extensive homology between human autosomal and X sequences. Hybridization to genomic probes that differ in species of origin indicate that these reiterated sequences have diverged between mouse and man. Eleven recombinants, each containing a different reiterated sequence(s), were hybridized in situ to metaphase chromosomes of mouse and man. These studies indicate that reiterated DNA which is homologous to the human X chromosome is more similar to DNA of human autosomes than to any murine chromosome. Therefore, it seems that reiterated DNA sequences on the human X chromosome have diverged as much during mammalian evolution as sequences on human autosomes. Moreover, the extensive modification of the original mammalian X has not interferred with the X inactivation process.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant / Q000378:metabolism*; D004275:DNA, Ribosomal; D005260:Female; D006801:Humans; D006822:Hybrid Cells / Q000502:physiology*; D007621:Karyotyping; D051379:Mice; D009693:Nucleic Acid Hybridization; D010920:Placenta; D011247:Pregnancy; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity; D014960:X Chromosome; D014981:Xenopus
D004274:DNA, Recombinant; D004275:DNA, Ribosomal; D004247:DNA