Alteration of plasma membrane of drug-resistant tumor cells: 230-kilodalton protein identified by monoclonal antibody.
A number of hybridomas producing antibodies to plasma membrane of an aclarubicin-resistant subline of L5178Y cells were obtained. Among the hybridoma antibodies, one was found by agglutination tests to react with the aclarubicin-resistant cell line, but not significantly with the parental and adriamycin-, bleomycin- and macromomycin-resistant cell lines. The monoclonal antibody was designated SC438, and showed complement-dependent cytolytic activity more markedly against the aclarubicin-resistant cells than against the parental cells. Fluorographs of [14C] leucine-labeled aclarubicin-resistant cells demonstrated two protein bands of 230k and 20k daltons, which were precipitated by the SC438 antibody. The former seemed to be specific for the aclarubicin-resistant cells.
D015250:Aclarubicin; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology*; D004351:Drug Resistance; D008223:Lymphoma / Q000276:immunology* / Q000503:physiopathology / Q000648:ultrastructure; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D008970:Molecular Weight; D009279:Naphthacenes / Q000494:pharmacology; D009374:Neoplasms, Experimental
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D008565:Membrane Proteins; D009279:Naphthacenes; D015250:Aclarubicin