Tumor promotion by dietary fat in azoxymethane-induced colon carcinogenesis in female F344 rats: influence of amount and source of dietary fat.
The promoting effect of dietary corn oil (CO), safflower oil (SO), olive oil (OO), coconut oil (CC), and medium-chain triglycerides (MCT) on azoxymethane (AOM)-induced colon tumors was studied in female F344 rats. The animals were fed low-fat diets containing 5% CO, 5% SO, or 5% OO 2 weeks before, during, and 1 week after sc injection of 20 mg AOM/kg body weight. One week after the AOM treatment, groups of animals were transferred to high-fat diets containing 23.52% CO, 23.52% SO, 23.52% OO, and 23.52% CC, or 5.88% CO + 17.64% MCT; the remaining animals were continued on 5% fat diets. All animals were fed these diets until the termination of the experiment. Body weights and intakes of calories, protein, and micronutrients were comparable among the various dietary groups. The incidence of colon tumors was increased in rats fed diets containing high-CO and high-SO compared to those fed low-CO and low-SO diets, whereas the diets containing high OO, CC, or MCT had no promoting effect on colon tumor incidence. There was a significant increase in the excretion of fecal deoxycholic acid, lithocholic acid, and 12-ketolithocholic acid in animals fed the high-CO and high-SO diets and no difference in these secondary bile acids excretion in animals fed the high-OO and high-CC diets compared to those animals fed their respective 5% fat diets. This study thus indicates that not only the amount of dietary fat but also the fatty acid composition (type) of fat are important factors in the determination of the promoting effect in colon carcinogenesis.
D000230:Adenocarcinoma / Q000139:chemically induced / Q000209:etiology; D000236:Adenoma / Q000139:chemically induced / Q000209:etiology; D000818:Animals; D001397:Azoxymethane; D001835:Body Weight; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000209:etiology*; D004041:Dietary Fats / Q000633:toxicity*; D005243:Feces / Q000032:analysis; D005260:Female; D007414:Intestinal Neoplasms / Q000139:chemically induced / Q000209:etiology*; D009821:Oils; D051381:Rats; D011916:Rats, Inbred F344; D013261:Sterols / Q000032:analysis
D004041:Dietary Fats; D009821:Oils; D013261:Sterols; D001397:Azoxymethane