Growth control in cultured 3T3 fibroblasts: neutralization and identification of a growth-inhibitory factor by a monoclonal antibody.
A fibroblast growth regulator, which inhibits the growth and division of proliferating fibroblasts, has been isolated from medium conditioned by exposure to density-inhibited mouse 3T3 cells. This partially purified preparation of the growth-inhibitory activity, termed FGR-s, contained two major polypeptides (Mrs, 10,000 and 13,000). Using FGR-s as the immunogen, we have carried out in vitro immunization of rat splenocytes and have generated hybridoma lines, each secreting an antibody directed against components of the FGR-s preparation. One such monoclonal antibody, designated 2A4, specifically bound the Mr 13,000 polypeptide of FGR-s. Antibody 2A4 also neutralized the growth-inhibitory effect of FGR-s in a concentration-dependent fashion. These results strongly suggest that the Mr 13,000 polypeptide carries growth-inhibitory activity.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002455:Cell Division; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D005260:Female; D006020:Glycopeptides / Q000302:isolation & purification* / Q000502:physiology; D006131:Growth Inhibitors / Q000302:isolation & purification*; D007700:Kinetics; D051379:Mice; D008970:Molecular Weight; D051381:Rats
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D006020:Glycopeptides; D006131:Growth Inhibitors; C020327:fibroblast proliferation inhibitor