Generation time of leukaemic blast progenitor cells.
Previous studies have indicated that the generation time of human leukaemic cells is longer than that of normal haematopoietic cells. We have employed a modification of the thymidine (TdR)-suicide technique to measure directly the generation time of leukaemic progenitor cells capable of colony formation. The results obtained with two human leukaemic cell lines (KG-1 and HL-60) and with blast progenitor cells from two patients with acute myelogenous leukaemia indicate generation times ranging from 9 X 0-22 X 0 hr and S-phase durations ranging from 5 X 5-8 X 0 hr. Using the same technique, the generation time of normal bone marrow CFU-c was determined to be 9-11 hr. These findings suggest that the proliferation rate of human leukaemic blast progenitor cells is similar to that of normal haematopoietic stem cells.
D000293:Adolescent; D000328:Adult; D002455:Cell Division; D002460:Cell Line; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D007399:Interphase; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D008297:Male; D014411:Neoplastic Stem Cells / Q000473:pathology*; D013234:Stem Cells / Q000473:pathology*