Developmental regulation of mRNA in mouse heart.
The myocardium contains abundant translatable mRNAs that change during development. Maximal cell-free synthesis of [3H]leucine-, [35S]methionine-, and [35S]cysteine-labeled translation products directed by poly(A)-containing mRNAs from 12-, 14-, and 17-day fetal; 5-day-old neonatal; and 30-day-old adult mouse heart was determined by using one- and two-dimensional polyacrylamide gels. Three general developmental patterns of heart-specific mRNA translation products were observed: two translatable mRNAs were most abundant in 12-day fetal heart; five mRNAs were most abundant in 14- and 17-day fetal heart and occurred only at low concentrations in 12-day fetal and adult heart; four mRNAs, including mRNAs coding for actin, tropomyosin, and myosin light chains 1 and 2, were most abundant in the adult heart. Thus, differentiating cardiac muscle is characterized by a complex pattern of mRNA regulation.
D000818:Animals; D002474:Cell-Free System; D005786:Gene Expression Regulation; D006321:Heart / Q000196:embryology*; D007526:Isoelectric Point; D051379:Mice; D008970:Molecular Weight; D009124:Muscle Proteins / Q000235:genetics*; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics
D009124:Muscle Proteins; D012333:RNA, Messenger