Compartmentalization of DNA polymerases, alpha and gamma, in maturing oocytes of the loach (Misgurnus fossilis L.).
The nuclei (germinal vesicles - GV) were isolated from the maturing oocytes of the loach (Misgurnus fossilis L.) by means of microsurgery. The activities of DNA polymerases, alpha and gamma, in the extracts of GV and enucleated oocytes were determined by two techniques: from crude extracts and following ultracentrifugation of the extracts in glycerol gradients. Nearly 80% of the total alpha-polymerase activity was shown to be associated with GV while the gamma-polymerase activity was revealed in the cytoplasm. The accumulation of non-histone nuclear proteins in the GV during oocyte maturation was demonstrated by means of autoradiography.
D000818:Animals; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D005260:Female; D005399:Fishes / Q000502:physiology; D008928:Mitochondria / Q000201:enzymology; D009865:Oocytes / Q000201:enzymology*
D004259:DNA-Directed DNA Polymerase