Disappearance of hypotetraploid clones after short-term culture of leukemic cells. A case report.
A case of M4-AML (acute monoblastic leukemia) is presented which was found to be hypotetraploid (79-80-? chromosomes per metaphase) with direct chromosome preparation while 24- and 48-hour cultures contained predominantly normal and some nonclonal hyperdiploid cells. This observation emphasizes the need for both direct and culture methods to disclose the true karyotype of leukemic cells.
D000328:Adult; D001853:Bone Marrow / Q000473:pathology; D002478:Cells, Cultured; D002871:Chromosome Banding; D002999:Clone Cells; D006801:Humans; D007621:Karyotyping; D007948:Leukemia, Monocytic, Acute / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D011003:Ploidies