Crystallization of myosin subfragment 1.
Crystals of myosin subfragment 1 from avian skeletal muscle have been grown reproducibly. They diffract x-rays to at least 4.5-A resolution. The subfragment 1 crystallizes in space group P2(1)2(1)2(1) where a = 107 A, b = 117 A, and c = 278 A. The cell dimensions and intensity distribution on x-ray diffraction photographs are consistent with two molecules in the crystallographic asymmetric unit. Electrophoretic analysis shows that the myosin subfragment 1 present in the crystals contains a 95-kilodalton heavy chain fragment and both the essential and regulatory light chains.
D000199:Actins; D000818:Animals; D002645:Chickens; D003460:Crystallization; D008854:Microscopy, Electron; D009132:Muscles / Q000032:analysis; D015879:Myosin Subfragments; D009218:Myosins; D010446:Peptide Fragments; D014961:X-Ray Diffraction
D000199:Actins; D015879:Myosin Subfragments; D010446:Peptide Fragments; D009218:Myosins