Alkaline phosphatase activity and tetracycline incorporation during initial orthodontic tooth movement in rats.
The activity of alkaline phosphatase and the incorporation of tetracycline as signs of bone formation were studied after orthodontic tooth movement for 10 h to 6 days in rats. Defined low or high forces were used. A moderate activity of non-specific alkaline phosphatase was found in the periodontal membrane (PDM) in untreated rats and in rats treated with low forces. In addition, all bone surfaces were outlined with a narrow band of intense non-specific alkaline phosphatase activity that was vanadate- and levamisole-resistant. Likewise, tetracycline was incorporated on all bone surfaces. The bone formation rate was low and uniform within the alveolus, indicating that no intra-alveolar drift of the molar occurred in the untreated rats. Orthodontic forces gradually inhibited vanadate- and levamisole-resistant alkaline phosphatases and tetracycline incorporation on the bone surfaces in the pressure zones of the PDM, depending on the magnitude of the force. It was suggested that the disappearance of these isoenzymes, in a limited area, as seen in the pressure zones, was associated with inhibited bone formation and subsequent initiation of bone resorption. On the tension side a slight reduction and redistribution of vanadate- and levamisole-resistant alkaline phosphatase activity could be noted irrespective of the magnitude of the applied force.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000539:Alveolar Process / Q000201:enzymology; D000818:Animals; D001846:Bone Development / Q000187:drug effects; D005260:Female; D006651:Histocytochemistry; D007978:Levamisole / Q000494:pharmacology; D010513:Periodontal Ligament / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013752:Tetracycline / Q000378:metabolism*; D013997:Time Factors; D014087:Tooth Movement Techniques; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology
D014639:Vanadium; D007978:Levamisole; D014638:Vanadates; D000469:Alkaline Phosphatase; D013752:Tetracycline