Tumor cytotoxicity of human macrophages after incubation with synthetic analogues of 2-lysophosphatidylcholine.
Human alveolar macrophages as well as macrophages derived from Teflon culture of blood-borne monocytes were incubated with synthetic analogues of 2-lysophosphatidylcholine and then tested for their cytotoxic capacity against an allogeneic lymphoma cell line. Metabolic, rather stable analogues enhanced macrophage cytotoxicity significantly. This phenomenon was shown both in a growth-inhibition assay as well as in the 51Cr release assay. Macrophage activation was dose- and time-dependent and was potentiated at temperatures above 37 degrees C. Incubation of the macrophages with the active compounds induced characteristic changes in cell morphology as revealed by scanning electron microscopy.
D000970:Antineoplastic Agents / Q000494:pharmacology; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D006801:Humans; D066298:In Vitro Techniques; D008223:Lymphoma / Q000276:immunology*; D008244:Lysophosphatidylcholines; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000276:immunology*; D010742:Phospholipid Ethers / Q000494:pharmacology*; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000494:pharmacology
D000970:Antineoplastic Agents; D008244:Lysophosphatidylcholines; D010742:Phospholipid Ethers; D010972:Platelet Activating Factor; C026659:edelfosine; C037145:1-hexadecyl-2-acetyl-glycero-3-phosphocholine; C026564:stearoyl alpha-lysolecithin; C043297:ilmofosine