Direct identification of a chemoattractant by a visual assay.
The purpose of this report was to describe an adaptation of the necrotactic assay for pharmacological studies. The target cell was substituted by microcapsules, neutral by themselves, which can be filled with well defined chemical substances. Some results obtained with the well known chemotactic substance formyl-methionyl-leucyl-phenylalanine and various inflammatory mediators (histamine, 5-hydroxytryptamine, PGE2 and PGF2 alpha) were exposed.
D000818:Animals; D002214:Capsules; D002630:Chemotactic Factors; D002634:Chemotaxis, Leukocyte; D015237:Dinoprost; D015232:Dinoprostone; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000502:physiology; D011458:Prostaglandins E / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D051381:Rats; D012701:Serotonin / Q000494:pharmacology
D002214:Capsules; D002630:Chemotactic Factors; D011458:Prostaglandins E; D011460:Prostaglandins F; D012701:Serotonin; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006632:Histamine; D015237:Dinoprost; D015232:Dinoprostone