Amino acid sequence of retinal transducin at the site ADP-ribosylated by cholera toxin.
Transducin was [32P]ADP-ribosylated by cholera toxin in bovine retinal rod outer segments and then partially purified on omega-amino octyl agarose to remove other ADP-ribosylated proteins. Trypsin digestion of the ADP-ribosylated transducin and further purification using boronate-polyacrylamide beads and high performance liquid chromatography yielded a single radiolabeled tetrapeptide, Ser-Arg-Val-Lys. The ADP-ribose is linked to the guanidinium group of arginine.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002772:Cholera Toxin / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D008565:Membrane Proteins / Q000032:analysis* / Q000378:metabolism; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D010786:Photoreceptor Cells / Q000032:analysis*; D012374:Rod Cell Outer Segment / Q000032:analysis*; D015293:Transducin; D014357:Trypsin / Q000378:metabolism
D008565:Membrane Proteins; D009702:Nucleoside Diphosphate Sugars; D000246:Adenosine Diphosphate Ribose; D002772:Cholera Toxin; D014357:Trypsin; D015293:Transducin