Activity of peroxidase and delta 5-3 beta-hydroxysteroid dehydrogenase in the ovary of LH-treated cyclic female rats.
Rats were treated with LH at 08:00 h on the first day of dioestrus or on Days 1 and 2 of dioestrus. Peroxidase activity increased within 3 h in females injected with LH on Day 1 and was associated with a depletion of ascorbate that lasted until the afternoon of Day 1. Values of both substances then returned to basal values by the morning of Day 2. A second LH injection on Day 2 produced effects similar to those seen after LH on Day 1. Females treated with LH did not display greater delta 5-3 beta-HSD activity than did controls on Day 1 after one LH injection, but did on Day 2 after two LH injections. Nonetheless, the changes were only modest by comparison with those of peroxidase. The peroxidase-ascorbate system appears to be involved in the mechanism responsible for the increased secretion of ovarian progesterone resulting from LH stimulation.
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000818:Animals; D001205:Ascorbic Acid / Q000097:blood; D004031:Diestrus; D005260:Female; D007986:Luteinizing Hormone / Q000494:pharmacology*; D010053:Ovary / Q000187:drug effects / Q000201:enzymology*; D010544:Peroxidases / Q000378:metabolism*; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D011374:Progesterone; D007986:Luteinizing Hormone; D015096:3-Hydroxysteroid Dehydrogenases; D010544:Peroxidases; D001205:Ascorbic Acid