Two cell-surface markers for human chromosome 1 in interspecies hybrids.
Monoclonal antibodies AbAJ9 and AbT87 define two distinct human cell-surface antigen systems (1, 2). Both antibodies react with a wide variety of cultured human cell types but not with rodent cell lines. AbAJ9 identifies a glycoprotein of 140,000 mol wt and AbT87 identifies a glycoprotein of 60,000 mol wt. The genetic control of antigen expression was studied in rodent-human somatic cell hybrids containing different subsets of the entire human chromosome complement, using an immune rosetting assay for serological analysis and hybrid selection. The presence of human chromosome 1 was found to be both necessary and sufficient for expression of antigens AJ9 and T87 in hybrid cells. Two independent gene loci on chromosome 1, designated MSK -1 for AJ9 and MSK -2 for T87 , control the expression of these antigens. We have assigned MSK -1 to region 1 cen-p22 and MSK -2 to region 1q32 -qter, using hybrids containing only fragments of human chromosome 1.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000954:Antigens, Surface / Q000235:genetics* / Q000302:isolation & purification; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002900:Chromosomes, Human, 1-3; D006023:Glycoproteins / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D006822:Hybrid Cells / Q000502:physiology; D008545:Melanoma; D051379:Mice; D008970:Molecular Weight; D009447:Neuroblastoma
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000954:Antigens, Surface; D006023:Glycoproteins