Cultivation of human osteosarcoma cell lines in serum-free hormone supplemented medium.
Two human osteosarcoma cell lines (TE-85, LM-1) were adapted to grow in serum-free hydrocortisone supplemented medium. Human osteosarcoma associated antigens were detected both on the cell membrane as well as in the concentrated culture medium. TE-85 cells produced bone specific alkaline phosphatase and LM-1 cells produced both bone specific and placental-like alkaline phosphatases when cultured in this medium. Successful proliferation of human osteosarcoma cell lines in serum-free medium is valuable in human osteosarcoma research.
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D007621:Karyotyping; D008854:Microscopy, Electron; D012516:Osteosarcoma / Q000201:enzymology / Q000235:genetics / Q000473:pathology*
D003470:Culture Media; D000469:Alkaline Phosphatase