Serum, platelet-derived growth factor, vasopressin and phorbol esters increase intracellular pH in Swiss 3T3 cells.
Addition of the growth-promoting agents phorbol esters, vasopressin, platelet-derived growth factor or fresh serum to quiescent cultures of Swiss 3T3 cells causes a significant increase in the uptake of 5,5-dimethyl oxazolidine-2-4-dione (DMO), a sensitive measure of intracellular pH. The results further indicate that cytoplasmic alkalinization is an early event associated with the action of a variety of mitogenic compounds.
D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D002273:Carcinogens / Q000494:pharmacology*; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D015240:Phorbol 12,13-Dibutyrate; D010703:Phorbol Esters / Q000494:pharmacology*; D010704:Phorbols / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D014667:Vasopressins / Q000494:pharmacology*
D002273:Carcinogens; D003470:Culture Media; D010703:Phorbol Esters; D010704:Phorbols; D010982:Platelet-Derived Growth Factor; D014667:Vasopressins; D015240:Phorbol 12,13-Dibutyrate