Quantitative profiling of prostaglandins and thromboxane by high-resolution gas chromatography-selected--ion monitoring.
The development and biological application of a rapid method for quantitative profiling of prostaglandins and thromboxane using high-resolution gas chromatography (HRGC) coupled with mass spectrometry in the selected-ion monitoring technique (SIM) are described. The method is based on the single-step extraction of prostaglandins from biological samples on C18 reversed-phase cartridges after addition of deuterated analogues as internal standards, followed by derivatization of functional groups and final analysis by HRGC-SIM with wall-coated open tubular persilanized capillary columns. Biological applications include the determination of endogenous arachidonic acid cascade profiles in rat tissue homogenates and thromboxane synthetase inhibition studies in human serum.
D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis; D000818:Animals; D015237:Dinoprost; D015232:Dinoprostone; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D015230:Prostaglandin D2; D011453:Prostaglandins / Q000032:analysis* / Q000097:blood; D011457:Prostaglandins D / Q000032:analysis; D011458:Prostaglandins E / Q000032:analysis; D011460:Prostaglandins F / Q000032:analysis; D051381:Rats; D013929:Thromboxane B2 / Q000032:analysis* / Q000097:blood; D013931:Thromboxanes / Q000032:analysis*
D011453:Prostaglandins; D011457:Prostaglandins D; D011458:Prostaglandins E; D011460:Prostaglandins F; D013931:Thromboxanes; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost; D015232:Dinoprostone; D015230:Prostaglandin D2