Chloroquine inhibits the production of a mononuclear cell factor by inhibition of lectin binding.
The effect of chloroquine (CQ) on the ability of normal human peripheral blood monocytes to produce a soluble mononuclear cell factor (MCF) capable of stimulating prostaglandins from adherent rheumatoid synovial cells was studied. CQ inhibited concanavalin A (Con A)- and bacterial peptidoglycan-induced MCF activity, but not colchicine-induced activity, in a dose-dependent manner. After incubation with CQ for 20 minutes, 3H Con A binding was reduced by 50%, and metabolism of pre-bound 3H Con A was inhibited. These data suggest that CQ can inhibit MCF production by inhibition of lectin binding, perhaps by inhibition of receptor recycling.
D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002738:Chloroquine / Q000494:pharmacology*; D003208:Concanavalin A / Q000378:metabolism; D015232:Dinoprostone; D006801:Humans; D015846:Monokines; D011458:Prostaglandins E / Q000096:biosynthesis; D014176:Protein Biosynthesis; D013583:Synovial Membrane / Q000166:cytology; D014316:Tritium
D015846:Monokines; D011458:Prostaglandins E; D014316:Tritium; D003208:Concanavalin A; D002738:Chloroquine; D015232:Dinoprostone