The interaction between lipid peroxidation and prostaglandin synthesis in rabbit kidney-medulla slices.
Lipid peroxidation induced by ascorbic acid and Fe2+ was inhibited by mepacrine (phospholipase A2 inhibitor) and aspirin (prostaglandin cyclo-oxygenase inhibitor) in rabbit kidney-medulla slices. Moreover, ascorbic acid and Fe2+ potentiated the inhibitory effect on prostaglandin E2 formation by mepacrine, but they had no influence on prostaglandin E2 production decreased by aspirin. Lipid peroxidation induced by ascorbic acid and Fe2+ appears to be affecting the activity of prostaglandin endoperoxide synthase. These results suggest that lipid peroxidation is connected closely with the prostaglandin-generating system, and it has the potential to modulate the turnover of arachidonic acid and prostaglandin synthesis.
D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D015232:Dinoprostone; D005227:Fatty Acids / Q000032:analysis; D066298:In Vitro Techniques; D007679:Kidney Medulla / Q000187:drug effects / Q000378:metabolism*; D050356:Lipid Metabolism; D008297:Male; D010545:Peroxides / Q000378:metabolism; D011458:Prostaglandins E / Q000096:biosynthesis*; D011796:Quinacrine / Q000494:pharmacology; D011817:Rabbits; D014280:Triglycerides / Q000378:metabolism
D005227:Fatty Acids; D010545:Peroxides; D011458:Prostaglandins E; D014280:Triglycerides; D011796:Quinacrine; D015232:Dinoprostone; D001241:Aspirin