Obtention of rat monoclonal antibodies reactive with human leukaemic lymphoblasts.
The Louvain rat-rat hybridoma technology was used to analyse membrane antigens present on human leukaemic lymphoblasts. Three fusions directed against non T non B leukaemic lymphoblasts finally yielded 42 distinct, stable, and specific antibody-secreting hybridomas. The cellular and molecular specificities of these monoclonal antibodies are still under investigation. However, a striking feature of our preliminary characterization is the obtention, in single fusions, of several distinct hybridomas that secrete antibodies binding to the same membrane molecule.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000951:Antigens, Neoplasm / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D000955:Antigens, T-Independent / Q000032:analysis; D002460:Cell Line; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D000955:Antigens, T-Independent