DNA-binding protein that induces cell differentiation.
Macrophage and granulocyte-inducing (MGI) proteins regulate the growth and differentiation of myeloid hematopoietic cells. One class of these proteins (MGI-1) induces cell growth and another class (MGI-2) induces cell differentiation. Results obtained with DNA-cellulose column chromatography have shown that the differentiation-inducing protein MGI-2 can bind to double-stranded cellular DNA, but that there was no such binding under the same conditions by the growth-inducing protein MGI-1. DNA binding may thus be used to separate MGI-2 from MGI-1. The MGI-2 from mouse bound to DNA from mouse and calf. There were different elution peaks of the MGI-2 bound to DNA suggesting a heterogeneity of MGI-2 molecules, and the last peak eluted from the DNA cellulose column was enriched for one of the molecular forms of MGI-2. After one further step of purification by polyacrylamide gel electrophoresis, this molecular form of MGI-2 was active at a concentration of 6.5 X 10(-11) M. In normal development MGI-1 induces MGI-2. This induction of a DNA-binding differentiation-inducing protein by a growth-inducing protein is an efficient mechanism for the normal coupling of growth and differentiation. It is suggested that this may also be a mechanism for the normal coupling of growth and differentiation in other types of cells.
D002454:Cell Differentiation; D002478:Cells, Cultured; D002482:Cellulose / Q000031:analogs & derivatives; D002846:Chromatography, Affinity; D002999:Clone Cells; D004247:DNA / Q000031:analogs & derivatives / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D007951:Leukemia, Myeloid / Q000503:physiopathology*
D004268:DNA-Binding Proteins; C033190:DNA-cellulose; D002482:Cellulose; D004247:DNA