A seven-base-pair deletion in an intron of the albumin gene of analbuminemic rats.
Analbuminemic rats, which genetically lack serum albumin, have a mutation affecting albumin mRNA processing. Serum albumin genes were cloned from analbuminemic and normal parental Sprague-Dawley rats. Structural analyses of the two albumin genes showed that the gene from analbuminemic rats had a seven-base-pair deletion in an intron. The deletion extended from base 5 to base 11 from the 5' end of intron HI of the albumin gene. This deletion converted the sequence, G-T-A-G-G-T, which is normally located at the 5' end of intron HI, to G-T-A-G-C-G. RNA blot hybridization of analbuminemic and normal rat liver nuclear RNA using a DNA fragment containing the intron HI as a probe showed that this intron sequence persisted in albumin mRNA precursors of analbuminemic rats.
D000818:Animals; D001483:Base Sequence; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005796:Genes; D012326:RNA Splicing; D051381:Rats; D011922:Rats, Mutant Strains / Q000235:genetics*; D012709:Serum Albumin / Q000172:deficiency* / Q000235:genetics
D012709:Serum Albumin