Magnetic susceptibility of oxy- and carbonmonoxyhemoglobins.
The room temperature magnetic susceptibilities of human and carp oxy- and carbonmonoxyhemoglobin solutions were measured in a 30-gauss (1 G = 10(-4) T) field with a superconducting magnetometer. To within experimental uncertainty, the susceptibility was the same for both the oxy and carbonmonoxy forms, and salt concentration did not effect it. A variety of sample preparations were used; the iron chemical state was verified by Mössbauer spectroscopy. A value of -0.580 +/- 0.010 X 10(-6) centimeter-gram-second (cgs) system was obtained for the mass susceptibility of the protein. We attribute the paramagnetism sometimes observed in oxyhemoglobin solutions to the presence of a small amount of the deoxy form.
D000818:Animals; D002263:Carboxyhemoglobin; D002347:Carps; D006418:Heme; D006454:Hemoglobins; D006801:Humans; D008280:Magnetics; D009994:Osmolar Concentration; D010108:Oxyhemoglobins; D013057:Spectrum Analysis
D006454:Hemoglobins; D010108:Oxyhemoglobins; D006418:Heme; D002263:Carboxyhemoglobin