Albumin secretion and protein synthesis by cultured diploid and tetraploid rat hepatocytes separated by elutriation.
Diploid and tetraploid rat hepatocyte subpopulations were isolated by elutriation and cultured for 24 h. Albumin secretion and protein synthesis rates were two-fold lower in 2n than in 4n hepatocytes. [35S]methionine-labelled proteins analysed by acrylamide gel electrophoresis showed a strikingly similar pattern in the two cell subpopulations. No differences in cellular proteins or in the intensity of labelling were observed. These results show (1) that viable diploid and tetraploid hepatocyte subpopulations can be separated by elutriation under sterile conditions and then cultured; and (2) strongly suggest that the same genes are transcribed and further translated at the same rate in both hepatocyte subpopulations.
D000418:Albumins / Q000378:metabolism*; D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008297:Male; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000418:Albumins