Separation of apolipoproteins of human very low density lipoproteins by chromatofocusing.
Chromatofocusing represents a new chromatographic procedure for the separation of proteins according to their isoelectric points. We describe the application of this method for the fractionation of the urea-soluble apolipoproteins of very low density lipoproteins. They were separated into five peaks, four of which were homogeneous as judged by polyacrylamide gel electrophoresis in the presence of 7 mol/l urea.
D001053:Apolipoproteins / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D007526:Isoelectric Point; D008079:Lipoproteins, VLDL / Q000032:analysis*; D012995:Solubility; D014508:Urea
D001053:Apolipoproteins; D008079:Lipoproteins, VLDL; D014508:Urea