Effect of metavanadate on the uptake and release of noradrenaline in rat brain cerebral cortex slices.
The effect of vanadium (as VO3-) on the uptake and release of tritiated noradrenaline ([3H]NA) was studied in vitro in rat cerebral cortex slices. Vanadate inhibited [3H]NA uptake and the inhibition was dependent upon concentration and on incubation time. The IC50 value (20 min incubation) was 8 X 10(-5) M of vanadate. Inhibition of Na+, K+ -ATPase activity by VO3-, chelation of noradrenaline or autooxidation of catecholamine by this oxyanion might contribute to the decrease of [3H]NA uptake. Vanadate inhibited also the release of [3H]NA in a time- and concentration-dependent fashion.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium; D014639:Vanadium / Q000633:toxicity*
D014639:Vanadium; D014316:Tritium; D009638:Norepinephrine