Dynorphins and Leu-enkephalin in brain nuclei and pituitary of WKY and SHR rats.
The distribution of dynorphin 1-13 (Dyn-1-13, Dyn-(1-8) and Leu5-enkephalin (LE) immunoreactivities (ir) were determined in discrete brain nuclei of normotensive (WKY) and hypertensive (SHR) rats. The concentration of ir-Dyn-(1-13) and ir-Dyn-(1-8) varied markedly among the various nuclei studies with a predominance of ir-Dyn-(1-13) over ir-Dyn-(1-8) in all the nuclei of both WKY and SHR rats. Ir-LE also showed large variations in different sites and no consistent relationships were found between the distribution of ir-Dyn-(1-8), Dyn-(1-13) and LE. SHR rats had lower levels of ir-Dyn-(1-13), Dyn-(1-8) and LE in the suprachiasmatic nucleus compared with WKY rats. In addition, SHR rats had lower levels of ir-Dyn-(1-8)- in the paraventricular and central amygdala, and higher ir-Dyn-(1-13) levels in the substantia nigra. The level of ir-Dyn-(1-13) in the neurointermediate lobe (NIL) of SHR rats was decreased substantially compared with that of WKY rats. The localization of these opioid peptides suggests that dynorphin-like peptides may serve a variety of hypothalamic and extrahypothalamic functions which might differ between SHR and WKY rats.
D000818:Animals; D001923:Brain Chemistry; D004399:Dynorphins; D004723:Endorphins / Q000032:analysis*; D004743:Enkephalin, Leucine / Q000032:analysis*; D006973:Hypertension / Q000378:metabolism*; D008297:Male; D010446:Peptide Fragments / Q000032:analysis*; D010902:Pituitary Gland / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D004723:Endorphins; D010446:Peptide Fragments; D004743:Enkephalin, Leucine; C026843:dynorphin (1-13); D004399:Dynorphins; C026770:dynorphin (1-8)