title: [Biological activity of tRNA, aminoacyl-tRNA-synthetases and composition of their high molecular weight complexes in regenerating rat liver].
abstract: tRNA isolated from the liver of rats with partial hepatectomy is established to possess an increased acceptor activity for amino acid mixture, lysine and glutamic acid 21 h after the operation and a decreased activity for tryptophan 6 and 15 h later when comparing control and experimental animals. The content of endogenic aminoacyl-tRNA in the liver of control and experimental animals in all periods of regeneration corresponds to the maximum level of tRNA aminoacylation in vitro. A complete correlation of changes in the tRNA activity and aminoacyl-tRNA-synthetases is observed only for glutamyl-tRNA-synthetase and tRNAGlu. High-molecular complexes of ARSases (codosomes) are isolated from liver of control and experimental animals 21 h after partial hepatectomy. The activity of lysyl- and glutamyl-tRNA-synthetases, content of tRNA, tRNAGlu,Lys as compared to the control is determined to rise, the content of phospholipids in their composition being 24% higher.
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D006498:Hepatectomy; D008099:Liver / Q000378:metabolism*; D008115:Liver Regeneration; D008297:Male; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases
vernacular_title: Biologicheskaia aktivnost' tRNK, aminoatsil-tRNK-sintetaz i sostav ikh vysokomolekuliarnykh kompleksov v regeneriruiushcheĭ pecheni krys.