Direct measurement of testosterone in a pediatric center, with use of a radioimmunoassay kit and unextracted serum.
We evaluated a commercial (Radioassay Systems) radioimmunoassay kit for testosterone in which a double-antibody method and an 125I tracer are used. The 50-microL serum sample is assayed directly with no pre-extraction step. The assay system has good sensitivity (0.01 microgram/L), good precision (CV 5.2%), and little cross reactivity between testosterone and other androgens. Results compared well with those by a tritiated radioimmunoassay involving extraction (r = 0.98). Values obtained for 97 children compared well with published pediatric normal values. Evidently, this kit provides a rapid and reliable measurement of testosterone in serum specimens and is acceptable for use with pediatric patients.
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003429:Cross Reactions; D013196:Dihydrotestosterone / Q000097:blood; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007457:Iodine Radioisotopes; D008297:Male; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic; D013223:Statistics as Topic; D013739:Testosterone / Q000097:blood*
D007457:Iodine Radioisotopes; D011933:Reagent Kits, Diagnostic; D013196:Dihydrotestosterone; D013739:Testosterone