In vitro growth promotion of T-lymphocyte colonies from normal human bone marrow with 12-O-tetradecanoylphorbol-13-acetate (TPA).
An in vitro culture system for the growth of T-lymphocyte colonies from normal human bone marrow has been described. This culture system features the use of 12-O-tetradecanoylphorbol-13-acetate (TPA) at moderately high concentrations (10(-7)-10(-6) M) and daily feeding with growth medium containing leukocyte-conditioned medium primed with phytohemagglutinin. T-lymphocyte colonies in this system can reach a size of over 10,000 cells/colony, suggesting that cells assayed in this system are early progenitors of T-lymphocytes with extensive proliferative capacity. TPA, at a higher concentration (10(-5) M), is inhibitory to the growth of T-lymphocytes, but this level is 5 orders of magnitude higher than the minimum TPA concentration (10(-10) M) inhibitory to myeloid colony forming cells. This demonstrates marked difference in the sensitivity of T-lymphocyte colony forming cells and myeloid colony forming cells to TPA. It appears that this assay system should be useful for the study of the early progenitors of human bone marrow T-lymphocytes.
D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D010704:Phorbols / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000166:cytology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D010704:Phorbols; D013755:Tetradecanoylphorbol Acetate