Isolation of an interleukin-1-like factor from human joint effusions.
Interleukin-1 (IL-1) is a macrophage derived mediator whose properties suggest that it could play a role in the pathology of arthritis. To test this hypothesis, joint fluids from patients with serveral different arthritides were tested. Small amounts of IL-1-like activity were recovered from many of these joint fluids after affinity chromatography over a column of rabbit anti-human IL-1. Positive fluids were obtained from patients with rheumatoid arthritis, psoriatic arthritis, Reiter's syndrome, osteoarthritis, gout, and traumatic arthritis. Upon gel filtration, the joint derived factor displayed a molecular weight distribution similar to that of IL-1 derived from human monocytes stimulated in vitro. These results suggest that IL-1 is present in joint effusions and, therefore, might contribute to joint destruction.
D001172:Arthritis, Rheumatoid / Q000378:metabolism; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis*; D013582:Synovial Fluid / Q000032:analysis*
D007375:Interleukin-1