Effect of toluene on the hemolytic resistance of rat erythrocytes.
The effect of toluene on rat erythrocyte hemolytic resistance was studied both in vitro and in vivo. In vitro, toluene at concentrations up to 1000 ppm showed a marked antihemolytic effect, the maximum being at 300 ppm. Above 1000 ppm, an increase in the hypotonic hemolysis was seen. The antihemolytic effect of toluene was temperature-dependent. Elevation of temperature diminished the ability of toluene to protect erythrocytes. In the in vivo experiments, when the rats breathed 2000 ppm of toluene in an inhalation chamber for 7 days and for 21 days (6 h/day), the antihemolytic effect of toluene was evident. Our results demonstrate that toluene, at moderate concentrations, increases the hemolytic resistance of rat erythrocytes in hypotonic media both in vitro and in inhalation exposures in vivo.
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology*; D006461:Hemolysis / Q000187:drug effects*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014050:Toluene / Q000633:toxicity*
D014050:Toluene