Solid-state extraction of cortisol from plasma or serum for liquid chromatography.
Solid-state extraction of plasma or serum offers significant advantages over traditional liquid-liquid methods in cortisol determinations by liquid chromatography (HPLC). Sample preparation is facilitated and HPLC column contamination is minimized. Extract yield from the solid-state columns is enhanced by sample pretreatment with methanol. Cortisol values by solid-state extraction/HPLC correlate well (r = 0.92) with those from a commercial radioimmunoassay procedure.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D006854:Hydrocortisone / Q000097:blood / Q000302:isolation & purification*; D000432:Methanol; D011863:Radioimmunoassay
D006854:Hydrocortisone; D000432:Methanol