Sézary cells with an unusual phenotype, their modulation with 12-O-tetradecanoyl phorbol-13-acetate (TPA) in vitro, and their relationship to T-cell development.
Leukemic cells from a patient with Sézary syndrome were studied with a panel of monoclonal antibodies. Despite their typical morphology, the cells expressed an unusual surface phenotype. They simultaneously expressed OKT4 and OKT8 reactivity, indicating a more immature origin of the neoplastic cells than what has previously been observed in Sézary syndrome, but they were OKT6 negative. In response to 12-O-tetradecanoyl phorbol-13-acetate (TPA) the cells underwent phenotypic alterations, as the OKT4 reactivity was significantly reduced and the OKT8 expression was retained. Thus, following TPA, the cells expressed a surface phenotype consistent with the cytotoxic/suppressor T-cell subset in normal peripheral blood. The findings are discussed in relation to normal T-cell maturation and differentiation processes.
D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D006801:Humans; D007938:Leukemia / Q000473:pathology; D008297:Male; D010641:Phenotype; D010704:Phorbols / Q000494:pharmacology*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D012751:Sezary Syndrome / Q000235:genetics / Q000473:pathology*; D013601:T-Lymphocytes / Q000166:cytology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D010704:Phorbols; D011947:Receptors, Antigen, B-Cell; D013755:Tetradecanoylphorbol Acetate