Separation of cholera enterotoxin-induced mucus secretion from electrolyte secretion in rabbit ileum by acetazolamide, colchicine, cycloheximide, cytochalasin B and indomethacin.
In vivo rabbit ileal loops were prepared and inoculated with purified cholera enterotoxin (CT). After a lag period of about 1 h there was persistent stimulation of water and electrolyte secretion and a transient stimulation of mucus secretion into the luminal fluid. Repeated intraluminal inoculation of prostaglandin E1 (PGE1) caused a pattern of water, electrolyte and mucus secretion which was qualitatively the same as that following CT, except that no lag period was observed. Doses of the protein synthesis inhibitor, cycloheximide, the microtubule disrupter, colchicine, and the microfilament disrupter, cytochalasin B, were found that inhibited CT-induced mucus secretion but not water and electrolyte secretion. The carbonic anhydrase inhibitor, acetazolamide, inhibited CT-induced water and electrolyte secretion without inhibiting the mucus secreted over a 5-hour test period. Thus a variety of agents can be used to demonstrate a separation of intestinal water and electrolyte secretion from mucus secretion. The prostaglandin synthesis inhibitor, indomethacin, also inhibited CT-induced water, electrolyte and mucus secretion, but no dose of this agent was found that completely separated the water and electrolyte from the mucus secretion.
D000086:Acetazolamide / Q000494:pharmacology; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D001826:Body Fluids / Q000502:physiology; D001834:Body Water / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D002772:Cholera Toxin / Q000494:pharmacology*; D003078:Colchicine / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D004573:Electrolytes / Q000378:metabolism*; D004768:Enterotoxins / Q000494:pharmacology*; D005260:Female; D006601:Hexoses / Q000378:metabolism; D007082:Ileum / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology; D007419:Intestinal Secretions / Q000187:drug effects*; D008297:Male; D009077:Mucins / Q000378:metabolism; D009093:Mucus / Q000378:metabolism*; D011817:Rabbits; D012964:Sodium / Q000378:metabolism; D014783:Viscosity; D014882:Water-Electrolyte Balance / Q000187:drug effects
D001639:Bicarbonates; D002712:Chlorides; D004573:Electrolytes; D004768:Enterotoxins; D006601:Hexoses; D009077:Mucins; D003571:Cytochalasin B; D002772:Cholera Toxin; D003513:Cycloheximide; D012964:Sodium; D000086:Acetazolamide; D003078:Colchicine; D007213:Indomethacin