Specificity of the suppressive action of glucocorticoids on the proliferation of monocyte/macrophages in the CSF-stimulated cultures of mouse bone marrow.
Addition of cortisol and its analogs to soft-agar culture of bone marrow cells markedly decreased the number of monocyte colonies in the presence of colony-stimulating factor. Steroids of other categories and cortisol metabolites were much less inhibitory than cortisol, and the shape of dose-response curve apparently differed between cortisol and other steroids. The action of cortisol was not influenced by excess progesterone or testosterone. Addition of cortisol succinate in liquid cultures of CSF-stimulated cells caused a gradual decrease of cellular uptake of [3H]thymidine. The steroid also suppressed CSF-independent [3H]thymidine uptake, but the time course of suppression obviously differed from that of CSF-dependent uptake. On day 7 of incubation, the steroid-treated cultures contained a smaller number of cells but higher activities of lysosomal enzymes than the control cultures. These results show that the glucocorticoids inhibit proliferation of the cells of monocyte/macrophage lineage.
D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000502:physiology* / Q000652:urine; D004357:Drug Synergism; D005938:Glucocorticoids / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006854:Hydrocortisone / Q000502:physiology; D008247:Lysosomes / Q000201:enzymology; D008264:Macrophages / Q000502:physiology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009000:Monocytes / Q000502:physiology; D011374:Progesterone / Q000494:pharmacology; D013739:Testosterone / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D003115:Colony-Stimulating Factors; D005938:Glucocorticoids; D013739:Testosterone; D011374:Progesterone; D013936:Thymidine; D006854:Hydrocortisone