[High molecular weight protein consisting of 14 monomers from pea leaves].
A new high molecular weight protein has been detected in pea leaves. Using electron microscopy it has been demonstrated that this protein consists of 14 identical monomers with a point 72 symmetry arranged in two layers, 7 monomers in each. The molecular weight of the protein as determined by gel filtration and sedimentation equilibrium method is equal to 900000 +/- 150000 and 950000 +/- 50000, respectively. The sedimentation coefficient for the protein is 24.3 +/- 1.0S. During SDS polyacrylamide gel electrophoresis the protein dissociates into identical polypeptide chains with molecular weight of 67000 +/- 3000. The circular dichroism spectra of the protein reveal that the percentage of alpha-helix portions, beta-structures, beta-turns and irregular portions is 0.45 +/- 0.06, 0.31 +/- 0.03, 0.09 +/- 0.03 and 0.15 +/- 0.07, respectively. The protein possesses a weak ATPase activity. The protein content in the leaves changes in the course of development.
D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae; D046911:Macromolecular Substances; D008970:Molecular Weight; D010940:Plant Proteins / Q000302:isolation & purification*; D010944:Plants / Q000032:analysis*; D010946:Plants, Medicinal; D011487:Protein Conformation
D046911:Macromolecular Substances; D010940:Plant Proteins
Sostoiashchiĭ iz 14 monomerov vysokomolekuliarnyĭ belok iz list'ev gorokha.