[Quantitative histophotometric study of liver dehydrogenase activity during temporary ischemia of the extremities].
This prospective study depicts quantitative histophotometric characteristics of the changes in the histoenzymologic spectrum of the liver in the course of temporary ischemia of the extremities with subsequent revascularization. The increase in the activities of dehydrogenases under study in the course of a 3-hour ischemia, and the decrease of these parameters in 6- and 12-hour ischemia was discovered, that was determined as a compensatory response followed by the period of compensation exhaustion and the development of the period of complete exhaustion. The data of the study of drug correction of the postischemia syndrome confirmed the rationality of the efforts that were made. A special program developed for computer processing of histological data allows rapid adaptation of the methods for different histophotometric examinations with the use of TV analyzer of the picture, this creating wider possibilities as compared to the known analogs.
D000818:Animals; D004285:Dogs; D005121:Extremities / Q000098:blood supply*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D007511:Ischemia / Q000201:enzymology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D010088:Oxidoreductases / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013997:Time Factors
D010088:Oxidoreductases; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase; D005969:Glutamate Dehydrogenase
Kolichestvenno gistofotometricheskoe izuchenie aktivnosti degidrogenaz pecheni pri vremennoĭ ishemii konechnosteĭ.