Glycosphingolipids in clonal variants of rat fibrosarcoma cells with different transplantability.
Neutral glycolipids and gangliosides of four clonal variants of rat fibrosarcoma AS-653 cells with different transplantability were analyzed. A highly malignant clone A had a much lower quantity of GM3 ganglioside and a much higher quantity of lactosylceramide as compared to clones Z and G, which showed low transplantability and less malignancy, and a variant clone P, which showed tumorigenicity only in ascites form. A similar correlation was found with the quantity of an unidentified slow-migrating neutral glycolipid in various clones. This glycolipid was present in trace amount in the original highly malignant clone A, increased moderately in clones G and Z, and increased greatly in clone P, which showed no subcutaneous transplantability. The results of these studies suggest that a blockage of synthesis of GM3 ganglioside and a long chain neutral glycolipid occurred with enhanced malignancy, and an enhanced synthesis of GM3 ganglioside and the long chain neutral glycolipid is associated with a decrease in transplantability.
D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005354:Fibrosarcoma / Q000032:analysis* / Q000473:pathology; D005732:Gangliosides / Q000235:genetics / Q000302:isolation & purification*; D006017:Glycolipids / Q000235:genetics / Q000302:isolation & purification*; D009368:Neoplasm Transplantation; D051381:Rats
D005732:Gangliosides; D006017:Glycolipids