The role of accessory cells in polyclonal T cell activation. I. Both induction of interleukin 2 production and of interleukin 2 responsiveness by concanavalin A are accessory cell dependent.
Recent studies from other laboratories have shown that concanavalin A (Con A) acts at two separate steps in polyclonal T cell activation: interleukin 2 (IL2) production, and induction of responsiveness to IL2. Using a combination of techniques for the depletion of accessory cells from lymph node T cells, we have investigated which of these steps, if not both, is responsible for the known requirement for accessory cells in the Con A response. It was found that with increasing T cell purification, first the ability is lost to produce sufficient levels of endogenous IL2, whereas induction of IL2 responsiveness can still take place. Further removal of accessory cells however yields a population of resting T cells that cannot be induced by Con A to become IL2-reactive. It was concluded that both IL2 production and induction of reactivity to IL2 are accessory cell-dependent events.
D000818:Animals; D003166:Complement Activating Enzymes / Q000276:immunology; D015922:Complement C1q; D003208:Concanavalin A / Q000494:pharmacology / Q000502:physiology; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000502:physiology; D007700:Kinetics; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008222:Lymphokines; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*
D000949:Histocompatibility Antigens Class II; D007106:Immune Sera; D007376:Interleukin-2; D008222:Lymphokines; C021649:concanavalin A-induced helper factors; D003208:Concanavalin A; D015922:Complement C1q; D003166:Complement Activating Enzymes