title: Characterization of bovine herpesvirus-1 isolated from trigeminal ganglia of clinically healthy cattle.
abstract: Isolates of bovine herpesvirus-1 (BHV-1) recovered from tissue explants of trigeminal ganglia of clinically healthy cattle were studied in vitro and in an animal model, and their characteristics were compared with those of vaccine and field strains of BHV-1. The isolates could be distinguished by their plaque size on cell monolayers, but were not significantly different in their thermal inactivation profiles at 48 C. Temperature-sensitive mutants were not found among the isolates when they were grown at 41 C. Selected isolates had different pathogenicity when inoculated in young rabbits.
mesh_terms: D000311:Adrenal Glands / Q000473:pathology; D000818:Animals; D002417:Cattle / Q000382:microbiology*; D006564:Herpesviridae / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D006358:Hot Temperature; D008099:Liver / Q000473:pathology; D009154:Mutation; D011817:Rabbits; D012668:Trigeminal Ganglion / Q000382:microbiology*; D014276:Trigeminal Nerve / Q000382:microbiology*; D010948:Viral Plaque Assay