[Ubiquinone-9 of the flagellates Crithidia oncopelti and Astasia longa].
The content of ubiquinones (Co Q) of the Astasia longa and Crithidia oncopelti protozoa was studied. The protozoa were grown on an artificial nutrient broth. The cells were separated, washed, freeze-dried, and refluxed with KOH and pyrogallol in ethanol media. The hydrolyzate was concentrated. The residue was stored at -20 degrees and filtered. An ubiquinone fraction was isolated from the filtrate by TLC on silica gel. Identification of the ubiquinone homologues was carried out by reverse phase TLC and mass spectrometry. Ubiquinones were quantitated with respect to the difference in the density between the oxidized and reduced forms of Co Q at 275 nm. The A. longa and C. oncopelti flagellates were shown to contain ubiquinone-9 (Co Q9) at a concentration of 0.48 and 1.14 mumole/g dry cells, respectively. The higher Co Q level in zooflagellates as compared to that in phytoflagellates is discussed.
D002855:Chromatography, Thin Layer; D003421:Crithidia / Q000032:analysis*; D056890:Eukaryota / Q000032:analysis*; D013058:Mass Spectrometry; D013056:Spectrophotometry, Ultraviolet; D014451:Ubiquinone / Q000032:analysis*
D014451:Ubiquinone; C030571:ubiquinone 9
Ubikhinon-9 flagelliat Crithidia oncopelti i Astasia longa.