Proliferation of chicken peripheral blood leukocytes in response to pokeweed mitogen is macrophage dependent.
Chicken peripheral blood leukocytes (PBL) proliferate in vitro in response to a wide range of pokeweed mitogen (PWM) concentrations. The PWM response was not influenced by the major histocompatibility complex (MHC) haplotype nor by the intrinsic responsiveness of the PBL to concanavalin A (Con A). The results indicate that PWM under our conditions stimulates B-lineage cells, although a T-cell subset is also clearly induced to division. The PBL from B-cell-depleted animals gave substantially lower responses than those from normal controls. Pokeweed mitogen stimulation of PBL was adherent cell dependent. Thus the low PWM response of adherent cell-depleted PBL was reconstituted by the addition of irradiated unseparated PBL. Furthermore, purified irradiated adherent cells containing greater than 90% macrophages were also able to reconstitute PWM responses. Finally, we have shown that PWM was able to induce large numbers of B cells to produce cytoplasmic immunoglobulin. However, only a minor proportion of such cells were induced to immunoglobulin secretion.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002448:Cell Adhesion; D002645:Chickens; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D007700:Kinetics; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology* / Q000528:radiation effects; D008297:Male; D011043:Pokeweed Mitogens / Q000494:pharmacology*; D013045:Species Specificity
D011043:Pokeweed Mitogens; D003208:Concanavalin A