title: The in vitro effect of phenytoin and carbamazepine on subpopulations of human blood mononuclear cells.
abstract: Mononuclear cells from peripheral blood of 10 healthy blood donors were incubated with phenytoin and carbamazepine dissolved in 1% propyleneglycol. Phenytoin 20 mg/1 significantly reduced the percentage of active E rosette-forming T-lymphocytes; to mean 17.6% compared to mean 25.9% in control incubations (P less than 0.05). Carbamazepine 10 mg/1 reduced this percentage to 21.0% (0.05 less than P less than 0.10). Phenytoin reduced the percentage of active T-lymphocytes in 9 of the 10 cell suspensions, while carbamazepine reduced the active T-lymphocytes in 8 of the 10 suspensions. The percentage of total E rosette-forming T-lymphocytes was similarly reduced by 20 mg/1 phenytoin and 10 mg/1 carbamazepine; from 65.3 to 59.5% and to 60.3%, respectively (P less than 0.05, 0.05 less than P less than 0.10). The reduction of active and total T-lymphocytes was concentration-dependent for both drugs. Phenytoin and carbamazepine 5 mg/1 did not influence the percentage of T-lymphocytes. The decrease became apparent at a concentration of 10 mg/1 for both drugs, and grew steadily more marked with increasing drug concentrations up to 80 mg/1. However, the reduction in the percentage of T-lymphocytes was more pronounced for phenytoin than for carbamazepine at all drug concentrations. The percentage of cells with Fc gamma-receptors and of those with receptors for the activated human complement component C3b, were not influenced by phenytoin and carbamazepine, nor were the cells with phagocytizing capacity and the non-phagocytizing cells with membrane-bound immunoglobulin (i.e. B-lymphocytes).
mesh_terms: D002220:Carbamazepine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009000:Monocytes / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects; D010672:Phenytoin / Q000494:pharmacology*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000187:drug effects*
chemical_list: D002220:Carbamazepine; D010672:Phenytoin