title: Effects of tumour promoter phorbol myristate acetate on mouse lymphocytes: selective inhibition of B cell activation by mitogens and antigens.
abstract: The effect of the tumour promoter phorbol myristate acetate (PMA) on the responses of mouse lymphocytes to various stimuli was studied in vitro. Nanomolar concentrations of PMA inhibited B cell proliferation induced by lipopolysaccharide and by anti-Ig antibodies, and the polyclonal antibody response to lipopolysaccharide. Specific antibody responses to both T-dependent and T-independent antigens were similarly affected. In marked contrast, T cell proliferation elicited by various mitogens was 10- to 1000-fold more resistant to inhibition. Cell fractionation experiments suggest that the effects on B cell responses are the results of a direct anti-proliferative effect of PMA on responding B lymphocytes.
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens / Q000494:pharmacology; D010704:Phorbols / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D008934:Mitogens; D010704:Phorbols; D013755:Tetradecanoylphorbol Acetate