Formation of a [99mTc]polypeptide hormone: characterization and chemical quality control by ampholyte displacement radiochromatography.
99mTc-complexes with the polypeptide hormone secretin in very low concentration were formed by the concentrated hydrochloric acid/vacuum evaporation/gentisic acid method. The 99mTc-secretin was characterized by a modified ampholyte radiochromatographic procedure, in addition to thin layer chromatography, gel chromatography and paper electrophoresis. High radiochemical purity and specific radioactivity were obtained. In vivo distribution studies were performed, and the conditions necessary for application of [99mTc]polypeptides as scintigraphic agents are discussed.
D000818:Animals; D007553:Isotope Labeling / Q000379:methods; D008667:Metalloproteins; D015609:Organotechnetium Compounds; D011786:Quality Control; D011817:Rabbits; D012633:Secretin / Q000097:blood / Q000378:metabolism; D013667:Technetium / Q000097:blood / Q000378:metabolism; D014018:Tissue Distribution
D008667:Metalloproteins; D015609:Organotechnetium Compounds; C039112:technetium Tc 99m secretin; D012633:Secretin; D013667:Technetium