Increased endogenous retroviral gene expression is a consequence of lymphocyte activation.
Plasma cells of line 151(5) chickens have been shown to express elevated levels of endogenous retroviral envelope glycoprotein (VEG), measured relative to levels expressed by both immature B cells and resting peripheral B lymphocytes. In this study we analyzed the relationship between peripheral blood lymphocyte (PBL) maturation and the level of VEG expression. A culture system was developed that would support maturation of pokeweed mitogen-activated peripheral B lymphocytes. As analyzed by cytofluorometry, both Ig+ and Ig- lymphoblasts present in the pokeweed mitogen-stimulated cultures expressed detectable levels of VEG in contrast to bursacytes and PBL. Similarly, Ig- blasts, which were present in concanavalin A-stimulated cultures of PBL and presumed to represent activated T cells, were also positive for the expression of VEG. Immature T cells, i.e., thymocytes, although negative by immunofluorescence analysis, expressed VEG at levels that were detectable by radioimmunochemical techniques. These results indicate that T cells as well as B cells constitutively express VEG, and that mitogenic activation of the resting lymphocyte induces an increase in VEG expression.
D000818:Animals; D001353:Avian Leukosis / Q000276:immunology; D001402:B-Lymphocytes / Q000032:analysis / Q000276:immunology; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002645:Chickens; D005434:Flow Cytometry; D008213:Lymphocyte Activation; D011043:Pokeweed Mitogens / Q000494:pharmacology; D012190:Retroviridae / Q000235:genetics* / Q000276:immunology; D013601:T-Lymphocytes / Q000032:analysis / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics*
D011043:Pokeweed Mitogens; D014759:Viral Envelope Proteins