title: Survival of frog striated muscle after pressure cooling.
abstract: Electronmicroscopical studies and functional tests (isometric contractibility) were performed in frog biceps muscle after treatment with elevated hydrostatic pressure at room temperature and at various temperatures below 0 degrees C. 700 bar pressure for 15 min. at room temperature caused rigor and damage to the sarcomers and mitochondria. 500 bar pressure for 15 min. and cooling to -17 degrees C resulted in solid freezing connected with severe damage to the muscular tissue and loss of any function. 518 bar pressure and cooling to -7 degrees C allowed good preservation of the ultrastructure and survival of the muscular tissue.
mesh_terms: D000818:Animals; D002470:Cell Survival; D005615:Freezing; D007537:Isometric Contraction; D008854:Microscopy, Electron; D009132:Muscles / Q000502:physiology* / Q000648:ultrastructure; D011312:Pressure; D011896:Rana temporaria; D014021:Tissue Preservation