Protein loss during nuclear isolation.
Cryomicrodissection makes possible the measurement of the entire in vivo protein content of the amphibian oocyte nucleus and provides a heretofore missing baseline for estimating protein loss during nuclear isolation by other methods. When oocyte nuclei are isolated into an aqueous medium, they lose 95% of their protein with a half-time of 250 s. This result implies an even more rapid loss of protein from aqueously isolated nuclei of ordinary-size cells.
D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis*; D005260:Female; D009865:Oocytes / Q000648:ultrastructure; D011506:Proteins / Q000032:analysis*; D014981:Xenopus
D011506:Proteins