Experimental beryllium-induced lung disease. II. Analyses of bronchial lavage cells in strains 2 and 13 guinea pigs.
In previous studies, we found that endotracheal administration of beryllium oxide (BeO) produced cell-mediated immunity and granulomatous lung disease in strain 2 but not strain 13 guinea pigs. This report describes phenotypic and functional studies of bronchial lavage cells from both guinea pig strains after BeO exposure. In BeO-treated strain 2 animals, the percentage of T (ER+)3 lymphocytes was significantly (p less than 0.05) elevated above normal by 2 weeks after BeO exposure, while T lymphocytes were not increased in strain 13. Giant cells containing crystalline material in fused, coalesced phagolysosomes were observed in treated animals of both strains. BeO-exposed strain 2 alveolar macrophages (AM) demonstrated enhanced killing of Listeria monocytogenes and enhanced chemiluminescence, while AM of strain 13 had responses below controls. Findings support the presence of BeO-induced cellular immunologic activity in strain 2 animals and, on the other hand, suggest BeO toxicity to lavage cells in strain 13.
D000818:Animals; D001608:Beryllium / Q000494:pharmacology; D005347:Fibroblasts / Q000473:pathology; D006168:Guinea Pigs; D008171:Lung Diseases / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D008213:Lymphocyte Activation / Q000187:drug effects; D008264:Macrophages / Q000201:enzymology / Q000276:immunology / Q000648:ultrastructure; D009113:Muramidase / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D011650:Pulmonary Alveoli / Q000276:immunology / Q000473:pathology*; D013601:T-Lymphocytes / Q000276:immunology; D007507:Therapeutic Irrigation
C032777:beryllium oxide; D009113:Muramidase; D001608:Beryllium