[Fluorometric method for the assay of IgG produced in lymphocyte cultures].
We have studied the IgG secreted in a system of CML. Microquantity of IgG (30-3000 micrograms/ml) of culture was determined using a fluorometric method based on the determination of the fluorescence derived by immune-complex IgG-FITC labeled goat Anti-human antibody to IgG covalently coupled to hydrophilic beads. This techniques has showed a good sensibility and accuracy.
D002478:Cells, Cultured; D005470:Fluorometry / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D008214:Lymphocytes / Q000276:immunology*
D007074:Immunoglobulin G
Metodo fluorimetrico per la valutazione delle IgG prodotte nelle colture linfocitarie.