Chromatin structure in barley nuclei.
In order to study the chromatin structure of a higher plant we used a high-yield method, which allows one to obtain up to 10(9) nuclei/kg fresh barley leaves. Significant amounts of low-ionic-strength-soluble chromatin can be extracted from these nuclei. Physicochemical properties were examined and discussed. Electric birefringence allowed us to observe the same transition in electro-optical properties as has been observed for animal chromatin, and suggested the existence of a symetrical structure occurring for approximately six nucleosomes. Circular dichroism showed that barley oligonucleosomes exhibit a higher molar ellipticity at 282 nm than total soluble chromatin and than their animal counterparts.
D001718:Birefringence; D055598:Chemical Phenomena; D002621:Chemistry; D002843:Chromatin / Q000302:isolation & purification*; D002942:Circular Dichroism; D004247:DNA / Q000302:isolation & purification*; D002523:Edible Grain / Q000032:analysis*; D006657:Histones / Q000302:isolation & purification; D001467:Hordeum / Q000032:analysis*; D012995:Solubility
D002843:Chromatin; D006657:Histones; D004247:DNA