Application of high pressure liquid chromatography and microsequencing methodology in the structural analysis of human hemoglobin variants.
This paper reports the results of the analyses of 17 different Hb variants which were observed in 37 families. High pressure liquid chromatography (HPLC) was used to separate tryptic and thermolytic peptides of globin chains. The double microsequencing technique can successfully be used for sequencing small or larger peptides.
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans
D006455:Hemoglobins, Abnormal