title: Lectin-resistant CHO cells: selection of new mutant phenotypes.
abstract: Cytotoxic plant lectins select for mutants which exhibit unique structural changes in surface carbohydrates reflecting specific defects in glycosylation reactions. However, lectins are not highly specific selective agents and, as a result, only the most frequently occurring mutants are obtained from single lectin selections. We have previously shown that the specificity of lectin selections may be improved by utilizing a combination of lectins added together or sequentially. This strategy has now been further exploited in the search for novel lectin-resistant mutants of Chinese hamster ovary cells. Five new LecR phenotypes have been uncovered. One belongs to a new, recessive complementation group, two behave dominantly in somatic cell hybrids, and the remaining two appear to represent new phenotypes which fall into previously described complementation groups.
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004351:Drug Resistance; D005260:Female; D005816:Genetic Complementation Test; D037102:Lectins / Q000494:pharmacology*; D009154:Mutation; D010053:Ovary / Q000166:cytology / Q000187:drug effects*; D010641:Phenotype; D012641:Selection, Genetic
chemical_list: D037102:Lectins