The stimulatory effect of oleate on citrulline formation from carbamoyl phosphate and ornithine in rat-liver mitochondria: the relation to ornithine uptake.
The effect of oleate, palmitate and octanoate on citrulline formation from carbamoyl phosphate nad ornithine was studied in rat liver mitochondria. In intact mitochondria, Oleate stimulated citrulline production for about 100%, while palmitate increased the rate of this process only for about 20-30% and octanoate had no significant effect. All the fatty acids studied did not change the rate of citrulline synthesis in sonicated mitochondria. Oleate increased significantly the mitochondrial ornithine uptake. The data indicate that the stimulatory effect of oleate on citrulline formation via ornithine carbamoyltransferase may be due to an increase of mitochondrial ornithine uptake.
D000818:Animals; D002210:Caprylates / Q000494:pharmacology; D002219:Carbamates / Q000378:metabolism*; D002221:Carbamyl Phosphate / Q000378:metabolism*; D002956:Citrulline / Q000096:biosynthesis*; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology*; D009952:Ornithine / Q000378:metabolism*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D002210:Caprylates; D002219:Carbamates; D009829:Oleic Acids; D010169:Palmitic Acids; D002956:Citrulline; D019301:Oleic Acid; D019308:Palmitic Acid; D002221:Carbamyl Phosphate; D009952:Ornithine; C031492:octanoic acid