Analysis of retinal and 3-dehydroretinal in the retina by high-pressure liquid chromatography.
A sensitive analytical method was developed in order to study the rhodopsin-porphyropsin system in the eye. Oximes of 11-cis-retinal, all-trans-retinal, 11-cis-3-dehydroretinal, and all-trans-3-dehydroretinal were determined quantitatively by high-pressure liquid chromatography. This method was applied to the analysis of retinal and 3-dehydroretinal in the retinas of bullfrog and goldfish. The results agreed with those obtained from the bleaching kinetics of visual pigment extracted with detergent. A reliable result is obtained if the tissue contains more than 5 pmol of retinal (or 3-dehydroretinal). The chromophore composition could be determined in the eye of a small freshwater prawn, Palaemon pancidence, using 50 pmol of 11-cis-retinal and no 3-dehydroretinal.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D033364:Decapoda; D006054:Goldfish; D010091:Oximes; D011892:Rana catesbeiana; D012160:Retina / Q000737:chemistry*; D012168:Retinal Pigments / Q000032:analysis*; D012172:Retinaldehyde / Q000031:analogs & derivatives* / Q000032:analysis*; D013045:Species Specificity; D014801:Vitamin A / Q000031:analogs & derivatives*
D010091:Oximes; D012168:Retinal Pigments; D014801:Vitamin A; C017184:dehydroretinal; D012172:Retinaldehyde