Correlation between alpha-amylase production and sporulation in Bacillus licheniformis CUMC 305 with respect to the effect of some carbohydrates and phenylmethylsulfonyl fluoride treatment.
The existence of any relationship between extracellular alpha-amylase production and sporulation in Bacillus licheniformis CUMC 305, when grown in the presence of different carbohydrates, was investigated. It was noted that alpha-amylase production in the organism was almost complete during the period of maximum sporulation, irrespective of the carbon source used. The effect of a sporulation inhibitor, phenylmethylsulfonyl fluoride at a concentration of 8 micrograms/ml, on enzyme secretion was an interesting feature. It was observed that alpha-amylase production as well as sporulation deteriorated, if the inhibitor was added to the external growth medium during the period extending from mid-logarithmic phase to mid-stationary phase of the growth cycle; contrary to it, there was no change in the pattern of alpha-amylase production or sporulation when added either earlier or later than this period. It may be concluded that the extracellular pool was the principal yield of alpha-amylase in the organism, since washing experiments and subsequent treatment of the intact cells with Tris hydroxymethyl aminomethane hydrochloride buffer or lysozyme, surfactant (Tween-80), or lysozyme-Tween mixture failed to extricate any substantial quantities of the enzyme. The intracellular pool of enzyme, obtained by disrupting the cells, was insignificant compared to the extracellular pool.
D001407:Bacillus / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D050260:Carbohydrate Metabolism; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology*; D013171:Spores, Bacterial / Q000502:physiology; D013450:Sulfones / Q000494:pharmacology*; D000516:alpha-Amylases / Q000096:biosynthesis*
D013450:Sulfones; D010664:Phenylmethylsulfonyl Fluoride; D000516:alpha-Amylases