Delayed-type skin hypersensitivity and in vitro lymphocyte immunostimulation responses of swine following inoculation with Mycobacterium avium cell walls and a mycobacterial immunopotentiating glycolipid.
Miniature swine (n = 5 per group) were inoculated intradermally with mineral oil-in-water emulsions containing either 150 micrograms of mycobacterial immunopotentiating glycolipid P3 (EP3), 150 micrograms of lyophilized Mycobacterium avium (serotype 8) cell walls (E-MaCW), or 150 micrograms P3 and 150 micrograms M. avium cell walls (EP3-MaCW). Swine vaccinated with E-MaCW and EP3-MaCW developed antigen-sensitive lymphocytes detectable with delayed-type hypersensitivity (DTH) skin tests and lymphocyte transformation assays. Swine injected with EP3 were not sensitized. In general EP3-MaCW evoked a more pronounced in vivo DTH tuberculin skin test and in vitro lymphocyte transformation responses than E-MaCW. Time-course studies indicated a more persistent response in swine injected with EP3-MaCW than in those given E-MaCW. Commercial type Yorkshire swine (n = 5) inoculated intradermally with EP3-MaCW developed cell-mediated immune (CMI) responses to avian tuberculin detectable in vivo with delayed-type skin hypersensitivity and in vitro with lymphocyte immunostimulation responses.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology / Q000494:pharmacology; D002473:Cell Wall / Q000276:immunology; D003311:Cord Factors / Q000276:immunology* / Q000494:pharmacology; D006017:Glycolipids / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology / Q000662:veterinary*; D007111:Immunity, Cellular; D007114:Immunization / Q000379:methods / Q000662:veterinary; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects*; D009161:Mycobacterium / Q000276:immunology*; D009162:Mycobacterium avium / Q000276:immunology* / Q000648:ultrastructure; D013552:Swine; D013553:Swine Diseases / Q000276:immunology; D013556:Swine, Miniature / Q000276:immunology*; D014376:Tuberculosis / Q000276:immunology / Q000662:veterinary; D014611:Vaccination / Q000379:methods / Q000662:veterinary
D000276:Adjuvants, Immunologic; D001428:Bacterial Vaccines; D003311:Cord Factors; D006017:Glycolipids