title: Comparative influence of hyperthermia and ionizing radiation on cycle and mitosis lengths in EMT6 mouse tumour cells.
abstract: With exponentially growing EMT6 mouse tumour cells in vitro, we have compared the effects of a dose of ionizing radiation and different temperature-time combinations on the lengths of the cell cycle and of mitosis using time-lapse cinematography on individual cells. The radiation dose and various temperature-time combinations all produced 50 per cent lethality. The hyperthermia treatments between 42 and 45 degrees C all caused a mitosis delay (MD) which was greater than that induced by ionizing radiation. The MD was greater with increasing temperature. In contrast, hyperthermia also induced a mitosis lengthening (ML) but this was only of the same order of magnitude as radioinduced ML. The results argue in favour of different mechanisms underlying thermoinduced MD and ML.
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000528:radiation effects*; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D003037:Cobalt Radioisotopes; D005720:Gamma Rays; D006358:Hot Temperature; D008321:Mammary Glands, Animal; D051379:Mice; D008938:Mitosis / Q000528:radiation effects; D009369:Neoplasms / Q000662:veterinary; D013997:Time Factors
chemical_list: D003037:Cobalt Radioisotopes