title: Selective loss of a plasma membrane protein associated with contraction of skeletal muscle.
abstract: Muscle proteins were labeled by incubating isolated frog sartorius muscles with [3H]- or [14C]phenylalanine. Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of plasma membrane fractions revealed a major protein band with an apparent molecular weight of approx. 96,000. Radioactivity in this band showed a clearly delineated decrease, relative to other bands, when previously labeled muscles were induced to contract either by electrical stimulation or by increasing the influx of Ca2+ from the incubation medium. It is postulated that a Ca2+-activated neutral protease may account for this decrease in labeled membrane protein.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008565:Membrane Proteins / Q000378:metabolism*; D009119:Muscle Contraction; D009124:Muscle Proteins / Q000378:metabolism*; D011894:Rana pipiens
chemical_list: D008565:Membrane Proteins; D009124:Muscle Proteins