Effects of long-term dietary modifications on pancreatic enzyme activity.
The effect of long-term dietary modification on pancreatic exocrine function in rats has been investigated by examining responses to cholecystokinin-pancreozymin stimulation, using a method of direct pancreatic duct cannulation. Residual pancreatic enzyme activity remaining in the whole pancreas following stimulation was also measured. After 6 months of dietary modification, changes in the pancreatic secretions of high-protein- or high-fat-fed animals were similar to those reported in short-term studies in which elevation of trypsin and lipase activity occurred in the pancreas. However, between 6 and 9 months, both volume and amylase activity of pancreatic secretions decreased in animals fed a high-protein diet. At 6 and 9 months, animals fed a high-carbohydrate diet had increased amounts of amylase and decreased trypsin in both pancreatic secretion and homogenate, similar to short-term changes previously reported. High-carbohydrate-fed animals had decreased lipase activity in pancreatic secretions, but lipase activity in pancreatic homogenates was increased, similar to levels found in animals fed a high-fat diet.
D000818:Animals; D001835:Body Weight; D002766:Cholecystokinin / Q000494:pharmacology; D004032:Diet; D004040:Dietary Carbohydrates / Q000008:administration & dosage; D004041:Dietary Fats / Q000008:administration & dosage; D004044:Dietary Proteins / Q000008:administration & dosage; D008049:Lipase / Q000378:metabolism; D009929:Organ Size; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology*; D010186:Pancreatic Function Tests; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical; D013997:Time Factors; D014357:Trypsin / Q000378:metabolism; D000516:alpha-Amylases / Q000378:metabolism
D004040:Dietary Carbohydrates; D004041:Dietary Fats; D004044:Dietary Proteins; D002766:Cholecystokinin; D008049:Lipase; D000516:alpha-Amylases; D014357:Trypsin