The effects of 2-(alpha-Tenoyl) thiopropionylglycine (TTPG) on human lung mucus.
2-(alpha-Tenoyl)-thiopropionylglycine (TTPG) was tested for its mucolytic activity on mucus specimens from human lungs. Our data demonstrate that a significant amount of mucus is solubilized in the presence of TTPG; this aspect is more evident when rat liver microsomes are incubated together with lung mucus and TTPG. Furthermore our data demonstrate the presence of thioesterase activity associated with liver, lung, kidney and brain microsomes capable of hydrolysing TTPG and thus liberating free thiol groups. Further studies are in progress to characterize the microsomal thioester activity as well as its probable role in regulating the viscosity of mucus.
D002499:Centrifugation, Density Gradient; D005100:Expectorants / Q000494:pharmacology*; D005998:Glycine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D008168:Lung / Q000187:drug effects*; D008861:Microsomes / Q000201:enzymology; D009093:Mucus / Q000378:metabolism*; D012995:Solubility; D013440:Sulfides; D013869:Thiolester Hydrolases / Q000378:metabolism; D013876:Thiophenes
D005100:Expectorants; D013440:Sulfides; D013876:Thiophenes; C035227:stepronin; D013869:Thiolester Hydrolases; D005998:Glycine