[Determination of plasma tocopherols using high performance liquid chromatography].
The authors describe an analytical method for the determination of plasma vitamin E by high performance liquid chromatography. 100 microliters of plasma are added to en ethanol-hexane mixture in presence of ascorbic acid. The hexan phase is concentrated and 50 microliters are injected directly into the chromatography in a silica gel column. The detector is set at 280 nm. The very low quantity of plasma required for the determination makes this method very useful in neonatal medicine.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D013237:Stereoisomerism; D014810:Vitamin E / Q000097:blood*
D014810:Vitamin E
Détermination des tocophérole plasmatiques par chromatographie en phase liquide haute performance.