title: Effect of S-100 protein on microtubule assembly-disassembly.
abstract: S-100 protein was examined for its ability to affect microtubule assembly-disassembly in vitro. Addition of S-100 protein inhibited microtubule assembly in a dose-dependent manner in the presence of Ca2+, but this effect was not observed in the presence of 2 mM EGTA. The interaction of microtubule proteins with S-100 protein was also demonstrated by Ca+-dependent affinity chromatography on S-100 protein-Sepharose. The proteins bound to the Sepharose column comigrated with tubulin as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These results might suggest a possible physiological role of S-100 protein in the microtubule assembly-disassembly system.
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology; D002417:Cattle; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D009418:S100 Proteins / Q000502:physiology*; D014404:Tubulin / Q000378:metabolism*
chemical_list: D009418:S100 Proteins; D014404:Tubulin; D002118:Calcium