Studies on T lymphocyte activation. II. Monoclonal antibody inhibiting the capacity of rat T lymphoblasts to absorb and to respond to IL-2: an anti-IL-2 receptor antibody?
We describe the production and some of the properties of a monoclonal antibody which inhibits binding of Interleukin 2 (IL-2) to rat T lymphoblasts as well as the capacity of these lymphoblasts to proliferate in response to IL-2 of rat or murine origin. According to these criteria, the antibody seems to be directed against an antigenic determinant on the IL-2 receptor. The antibody, obtained from hybridomas derived from mice immunized with phorbol-myristate-acetate-pulsed rat T lymphoblasts, exhibited species specificity, indicating that IL-2 receptors of various species are not necessarily identical, even if they can respond to IL-2 from different species.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D005260:Female; D007376:Interleukin-2 / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011919:Rats, Inbred Strains; D011971:Receptors, Immunologic / Q000276:immunology*; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D011971:Receptors, Immunologic