Two-quantum selective laser scission of polyadenilic acid in the complementary complex with a dansyl derivative of oligothymidilate.
Nonathymidilate was synthesized containing the chromophore (dansyl) group linked to its 5'-phosphate. In the presence of this compound the polyadenilic acid molecules are split by the radiation (power density J greater than or equal to 70 MW/cm2) of a nitrogen laser, while under the same conditions poly(C) and poly(U) are hardly affected. This selective optically non-linear effect was predicted and is explained in terms of radiativeless transfer of two-quantum excitation of the chromophore which is fixed on poly(A) molecule due to the formation of the complementary complex with nonathymidilate.
D055598:Chemical Phenomena; D002621:Chemistry; D007700:Kinetics; D007834:Lasers; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D009841:Oligonucleotides / Q000138:chemical synthesis; D011061:Poly A; D011789:Quantum Theory
D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; C027903:oligo (dT); D011061:Poly A