title: The effects of ionizing radiation on the peroxide content of a pure polyunsaturated lipid dispersion and of lipids and membranes derived from Acholeplasma laidlawii.
abstract: Dispersions of a pure unsaturated phospholipid, dilinoleoylphosphatidyl choline, formed conjugated diene hydroperoxides when irradiated in air with 7 MeV electrons (150 Gy and 300 Gy). Peroxide formation was optimized when the dispersions were irradiated in air at 37 degrees C at a dose rate of 5 Gy/min. No significant loss of linoleic acid from the irradiated phospholipid dispersions was observed after doses of 150 or 300 Gy. Small amounts of thiobarbituric acid-reactive material were formed in irradiated unsaturated phospholipid dispersions. However, lipids or membranes isolated from 48 hour cultures of Acholeplasma laidlawii grown in media supplemented with either linoleic or linolenic acid did not appear to be peroxidized by irradiation under the same conditions.
mesh_terms: D000128:Acholeplasma laidlawii / Q000032:analysis / Q000166:cytology; D002462:Cell Membrane / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004583:Electrons; D008054:Lipid Peroxides / Q000096:biosynthesis*; D008055:Lipids / Q000528:radiation effects*; D010315:Particle Accelerators; D010713:Phosphatidylcholines / Q000528:radiation effects*; D013997:Time Factors
chemical_list: D008054:Lipid Peroxides; D008055:Lipids; D010713:Phosphatidylcholines; C002448:1,2-linoleoylphosphatidylcholine