Purification to apparent homogeneity of a thymocyte specific growth factor from calf thymus.
A thymic peptide previously found to recruit thymocytes from G1 into S phase has been purified from a crude thymic extract by subsequent steps of gel exclusion chromatography and reverse phase high performance liquid chromatography (HPLC). The purified material, which appeared homogeneous on thin-layer chromatography and HPLC, stimulated the DNA synthesis of cultured guinea pig thymocytes in a nanomolar concentration range. The amino acid composition revealed a high content of acidic amino acids and no apparent homology to previously defined growth factors and thymus differentiation hormones.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001681:Biological Assay; D002417:Cattle; D002455:Cell Division; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000096:biosynthesis; D006133:Growth Substances / Q000302:isolation & purification* / Q000494:pharmacology; D006168:Guinea Pigs; D013601:T-Lymphocytes / Q000166:cytology*; D013950:Thymus Gland / Q000032:analysis*
D000596:Amino Acids; D006133:Growth Substances; D004247:DNA