DNA-mediated gene transfer into human diploid fibroblasts derived from normal and ataxia-telangiectasia donors: parameters for DNA transfer and properties of DNA transformants.
We investigate the feasibility of DNA-mediated gene transfer into human diploid fibroblasts derived from patients with the radiation sensitive syndrome ataxia-telangiectasia (A-T) and from a normal donor. Although they are markedly different in their growth characteristics, both normal and A-T strains give similar frequencies for DNA transfer in a model system using the recombinant plasmid pSV2 -gpt. pSV2 -gpt DNA transformants arise with a frequency between 10(-5) and 10(-4) per viable cell. Analysis of such transformants, although possible, is severely handicapped by the limited clonal life span of diploid human cells. Despite these problems it may be concluded that diploid human fibroblasts are competent recipients for DNA-mediated gene transfer and the putative repair deficiency of A-T does not markedly effect the efficiency of this process.
D001260:Ataxia Telangiectasia / Q000235:genetics*; D004274:DNA, Recombinant; D004171:Diploidy; D005347:Fibroblasts; D006801:Humans; D010957:Plasmids; D014170:Transformation, Genetic
D004274:DNA, Recombinant