Changes of platelet cell volumes in hypotonic solution.
Changes of platelet cell volumes in hypotonic solution were studied by the use of a new apparatus for cell volume analysis. Continuous mean cell volume analyzer (CMA) is a computerized instrument which continuously counts the mean cell volume and records the results. Moreover, it also shows patterns of expansion or shrinkage. Adequate amounts of platelet rich plasma were added into 0.8 or 0.4% of NaCl solution containing 5mM phosphate buffer. The mean platelet volumes were measured continuously every 2 seconds for 200 seconds. In isotonic solution, platelets changed their volumes little, but in hypotonic solution, the volumes increased for about 20 seconds and then, decreased gradually. Platelet volume expansion ratio and shrinkage ratio were calculated from these patterns. The shrinkage ratio well corresponded to the values of hypotonic shock response measured by spectrophotometry (r = 0.861). Expansion and especially shrinkage ratios decreased during storage, and they were dependent on stored temperature and pH.
D001792:Blood Platelets / Q000648:ultrastructure*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007038:Hypotonic Solutions; D008855:Microscopy, Electron, Scanning; D012965:Sodium Chloride; D013696:Temperature
D007038:Hypotonic Solutions; D012965:Sodium Chloride