Isolation and functional property of mRNAs coding for human interleukin 2.
Interleukin 2 (IL 2) is a lymphokine with specific growth promoting properties for activated T cells. The isolation and purification of specific mRNAs coding for this protein is reported. The analysis of total poly A + containing RNA from PHA stimulated T lymphocytes on agarose gels reveals the existence of at least two different IL-2 mRNAs. Isolated RNAs have been microinjected separately into Xenopus laevis oocytes for translation and consecutively the synthesized proteins were tested for activity on alloactivated T cell lines which were dependent on IL-2 for growth. Both RNAs coded for a protein which displayed clearly detectable IL-2 activity.
D000818:Animals; D002478:Cells, Cultured; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000235:genetics*; D009865:Oocytes / Q000378:metabolism; D011061:Poly A / Q000032:analysis; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D013601:T-Lymphocytes; D013936:Thymidine / Q000378:metabolism; D014982:Xenopus laevis
D007376:Interleukin-2; D012333:RNA, Messenger; D011061:Poly A; D013936:Thymidine