Measurement of DNA with an automatic spectrophotometer.
A method that uses an automated spectrophotometer to read microtiter plates in a modification of the diphenylamine method of DNA determination in tissue extracts, is presented. The micromethod saves technician time, and reduces exposure to acetic acid fumes. The reproducibility of the method is superior to the manual method. Fewer pipetting steps are required, and the method is suited to large batches of samples.
D000818:Animals; D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods; D004247:DNA / Q000032:analysis*; D004159:Diphenylamine; D051381:Rats; D013053:Spectrophotometry / Q000295:instrumentation; D014020:Tissue Extracts / Q000032:analysis*
D014020:Tissue Extracts; D004247:DNA; D004159:Diphenylamine