Simple test system for interleukin-2.
A simple system was developed to evaluate tests using the principle of the multiplication of specific cells. Of the possible application such as hormones, growth factors, etc., we show here the determination of IL-2 activity. The test is performed in flat bottom microplates wherein the sample is diluted with standard microtiter systems and cells are added. After the incubation period cells are fixed to the surface and stained. The absorbed stain is eluted and the optical density measured with a Titertec Multiscan photometer. Results correspond well to commonly used radiotracer systems and are as sensitive as those but much easier, cheaper and quicker to perform.
D000818:Animals; D001399:Azure Stains; D001681:Biological Assay / Q000379:methods; D002460:Cell Line; D007376:Interleukin-2 / Q000032:analysis*; D051379:Mice; D013602:T-Lymphocytes, Cytotoxic
D001399:Azure Stains; D007376:Interleukin-2