Repeated skin tests in guinea pigs with fluorescein visualization.
A method is presented for immediate hypersensitivity skin testing of guinea pigs using fluorescein as a contrast medium. The method allows repeated skin tests of individual animals. Results are compared with those obtained using Evans blue dye, which leaves animals unsuitable for later skin testing. The two methods give very similar direct skin test results.
D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D004306:Dose-Response Relationship, Immunologic; D005070:Evans Blue; D005260:Female; D019793:Fluorescein; D005452:Fluoresceins / Q000032:analysis; D006168:Guinea Pigs; D006632:Histamine; D006969:Hypersensitivity, Immediate / Q000175:diagnosis* / Q000473:pathology; D010047:Ovalbumin / Q000276:immunology; D012882:Skin Tests / Q000379:methods*
D000941:Antigens; D005452:Fluoresceins; D005070:Evans Blue; D006632:Histamine; D010047:Ovalbumin; D019793:Fluorescein