Antibody responses to Haemophilus influenzae type b and diphtheria toxin induced by conjugates of oligosaccharides of the type b capsule with the nontoxic protein CRM197.
Oligosaccharides were made from Haemophilus influenzae type b capsular polysaccharide and conjugated to CRM(197) by reductive amination. Conjugates were made with a range of lengths and multiplicities of saccharide chains. All elicited a strongly enhanced anti-H. influenzae type b capsular polysaccharide response when injected into weanling rabbits. One series of conjugates also elicited antibodies to diphtheria toxin.
D000818:Animals; D000917:Antibody Formation; D001426:Bacterial Proteins / Q000276:immunology*; D004167:Diphtheria Toxin / Q000276:immunology*; D006193:Haemophilus influenzae / Q000276:immunology*; D009844:Oligosaccharides / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011817:Rabbits
D001426:Bacterial Proteins; D004167:Diphtheria Toxin; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; C028581:CRM197 (non-toxic variant of diphtheria toxin)