DMSO increases tyrosine residue phosphorylation in membranes from murine erythroleukemia cells.
Phosphorylation of membranes from murine erythroleukemia cells was performed in the presence and absence of the polar solvent dimethyl sulfoxide. Quantitation of the phosphoamino acid content revealed that DMSO stimulated phosphotyrosine accumulation by three-fold; serine and threonine phosphorylation decreased significantly. We had previously shown that DMSO stimulated tyrosine residue phosphorylation of the hepatic epidermal growth factor receptor. EGF had little effect in MEL membranes; therefore, DMSO results in accumulation of phosphotyrosine in cell membranes that do not exhibit significant EGF-dependent phosphorylation.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism; D051379:Mice; D010766:Phosphorylation; D014443:Tyrosine / Q000378:metabolism*
D014443:Tyrosine; D004815:Epidermal Growth Factor; D004121:Dimethyl Sulfoxide