title: Relative subunit composition of the ferritin synthesized by selected human lymphomyeloid cell populations.
abstract: Biosynthesis of ferritin subunits by cell sets isolated from normal human peripheral blood, spleens of Hodgkin's disease patients, and tumor cell lines were investigated. Normal mature hematopoietic cells made a ferritin with more H (21K) than L (19K) subunits. The reverse was found for a promyelocytic tumor cell line and tumor cell lines derived from other tissues. Two dimensional electrophoresis indicated H has a lower pI than L. Therefore relative proportions of the two subunits contribute to the electrophoretically distinct forms of the isoferritins. In response to increasing concentrations of iron in vitro, a selected monocyte population synthesized more H than L; L biosynthesis however increased more than H. Some possible regulatory implications of these observations are discussed.
mesh_terms: D002460:Cell Line; D005290:Ferric Compounds / Q000494:pharmacology; D005293:Ferritins / Q000096:biosynthesis* / Q000302:isolation & purification; D006098:Granulocytes / Q000378:metabolism; D006689:Hodgkin Disease / Q000032:analysis / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008214:Lymphocytes / Q000032:analysis / Q000378:metabolism*; D008970:Molecular Weight; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000032:analysis / Q000378:metabolism
chemical_list: D005290:Ferric Compounds; D005293:Ferritins