Limitations of standard filtration techniques in measuring specific binding of transported substrates to renal plasma membranes.
Under equilibrium conditions, the specific binding of 14C-alpha-methyl-D-glucoside and 14C-p-aminohippurate to brushborder and basolateral membranes of rabbit kidney cortex could not be quantitated using standard filtration techniques. It was concluded that the low receptor affinities of these transported solutes and the masking of bound isotopic counts by vesicular uptake prevented accurate measurement of specific binding activities.
D000618:Aminohippuric Acids / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000378:metabolism*; D005374:Filtration / Q000379:methods; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000378:metabolism*; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D011817:Rabbits; D010130:p-Aminohippuric Acid / Q000378:metabolism*
D000618:Aminohippuric Acids; D002250:Carbon Radioisotopes; D008757:Methylglucosides; D008759:Methylglycosides; C027020:methylglucoside; D010130:p-Aminohippuric Acid