The development of specific antibody-containing cells in the spleen of rabbits during the secondary immune response against free or liposome-associated albumin antigen.
In order to study the distribution pattern of specific antibody-containing cells in the spleen of rabbits during the secondary immune response, rabbits were given two intravenous injections of either free or liposome-associated human serum albumin (HSA) within an interval of 2 months. Demonstration of specific antibody-containing cells was performed by incubation of sections of spleen with HSA-horseradish peroxidase (HRP) conjugates, followed by peroxidase cytochemistry. Specific anti-HSA antibody-containing cells were detected already within 2 days after booster and peak numbers were found 4 days after booster. The bulk of these cells localized in the coaxial lymphocyte sheaths surrounding the terminal arterioles in the spleen. Specific antibody-containing cells were also found in the follicles. Using a double immunoenzyme technique we demonstrated that a majority of the specific antibody-containing cells produced immunoglobulin G(IgG) antibodies. From the results, it is also concluded that, after a priming injection with liposome-associated HSA, liposomes do not further enhance the secondary immune response, when they are also used for the booster injection.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000888:Antibodies, Anti-Idiotypic / Q000145:classification; D000917:Antibody Formation; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology*; D002682:Chinchilla; D008081:Liposomes; D008297:Male; D011817:Rabbits / Q000276:immunology*; D012709:Serum Albumin / Q000276:immunology*; D013154:Spleen / Q000033:anatomy & histology / Q000166:cytology / Q000276:immunology*
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D008081:Liposomes; D012709:Serum Albumin