The biochemical study of rat T-cell growth factor (interleukin 2).
T-cell growth factor (interleukin 2) was partially purified from the conditioned medium from Con A-stimulated rat splenocytes. It had the molecular weight of 23,000 according to gel filtration and in the range of 17,000-18,000 daltons according to PAGE in the presence of SDS. The molecular forms of IL 2 exhibited isoelectric points (pI) of 5.4-5.8 (major peak), 4.3-4.5 and 6.5-6.8 (minor peaks). T-cell growth-promoting activity was remarkably stable to temperature, pH, SDS and 2-ME treatments. Rat IL 2 did not bind to immobilized Con A, WGA, RC 1, and fucose-binding protein. The conditioned medium also contained the inhibitor of IL 2-dependent T-cell proliferation.
D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007376:Interleukin-2 / Q000032:analysis* / Q000302:isolation & purification; D007525:Isoelectric Focusing; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology
D007376:Interleukin-2