Purification and properties of genetic variants of mouse trypsinogen.
The presence of three trypsinogens (Try-III, Try-I, and Try-II) in the mouse is demonstrated by DEAE-cellulose column chromatography. Two genetic variants of Try-I are detected, because the activity of Try-I is different between the Mol-A strain and seven other strains. The Prt-3 locus controls the activity of Try-I. The Prt-3a gene exists in CFO, BS, KR, BALB/cJ, C57BL/6J, CBA/J, and 129/Sv-S1-CP strains, whereas the Prt-3b gene is present only in the Mol-A strain. Each Try-I from the CFO or Mol-A strain was purified. The fact that Try-I activity of the Mol-A strain is much higher than those of other strains is because of a difference in the specific activity; the ratio of the Kcat (sec-1) value with Tos-Arg-OMe to that with Bz-Arg-OEt is different between the variants from the CFO and those from the Mol-A strains, being much higher in the Mol-A strain. Also, chicken ovomucoid inhibited Try-I activity of the CFO strain at a molar ratio of one ovomucoid to two trypsins; Try-I activity of the Mol-A strain was only 50% inhibited even with an excess of ovomucoid. There was no difference between genetic variants of Try-I in molecular weight, Km values with Bz-Arg-OEt or Tos-Arg-OMe, pH optimum profile, or inhibition by soybean trypsin inhibitor.
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives; D014644:Genetic Variation; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D013045:Species Specificity; D013379:Substrate Specificity; D014106:Tosylarginine Methyl Ester; D014361:Trypsin Inhibitors / Q000494:pharmacology; D014362:Trypsinogen / Q000235:genetics* / Q000378:metabolism
D014361:Trypsin Inhibitors; D014362:Trypsinogen; D014106:Tosylarginine Methyl Ester; D001120:Arginine; C009727:benzoylarginine ethyl ester