Purification of primate T cell growth factor to apparent homogeneity by reverse phase high pressure liquid chromatography: evidence for two highly active molecularly distinct species.
T cell growth factor produced by the MLA144 gibbon ape lymphosarcoma T lymphocyte line was separated into two molecular forms of Mr 16,300 and Mr 14,300 which were purified 32,000-fold and 82,000-fold, respectively, and which in combination constitute the majority of biological activity of the starting material. The overall recovery of biological activity was 50%. This was accomplished with a series of chromatographic steps including reverse-phase high pressure liquid chromatography. The purified proteins are of comparable specific activities and each maintains the DNA synthesis of T cell growth factor-dependent human T cell lines at concentrations of less than approximately 18 pM (300 fg/ml).
D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004261:DNA Replication / Q000187:drug effects; D006801:Humans; D006922:Hylobates; D007376:Interleukin-2 / Q000302:isolation & purification*; D008228:Lymphoma, Non-Hodgkin / Q000032:analysis; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000187:drug effects
D007376:Interleukin-2