title: An inexpensive, microcomputer-based, video densitometer for quantitating thin-layer chromatographic spots.
abstract: An inexpensive video densitometer based on an Apple II microcomputer using commercially available components was developed. The device was used to quantitate charred lipid and colored spots on thin-layer chromatography plates. The method is useful for lipid samples weighing 0.1 to 4 micrograms on high-performance plates and 1 to 20 micrograms on ordinary plates. The software automatically corrects for baseline drift due to dirt or nonuniformity in the plate.
mesh_terms: D002518:Ceramides / Q000032:analysis; D002784:Cholesterol / Q000032:analysis; D002855:Chromatography, Thin Layer / Q000295:instrumentation* / Q000379:methods; D003201:Computers / Q000191:economics; D003720:Densitometry / Q000295:instrumentation*; D008838:Microcomputers / Q000191:economics
chemical_list: D002518:Ceramides; D002784:Cholesterol