Selective recording and stimulation of individual identified neurons in freely behaving Aplysia.
A neuroethological technique is described for selective recording and stimulation of an individual neuron in freely behaving Aplysia by means of a fine wire glued into the connective tissue sheath above the identified cell body. A whole-nerve cuff electrode simultaneously monitored functionally related multiunit axon activity. For biophysical analysis the soma was impaled with a microelectrode when the ganglion was subsequently exposed. The technique is illustrated for several identified neurons involved in different behaviors.
D000818:Animals; D001048:Aplysia; D004558:Electric Stimulation / Q000379:methods; D008839:Microelectrodes; D009474:Neurons / Q000502:physiology*