Non-cytotoxic alloantibodies defined by the EA rosette inhibition assay.
Sera from both transfused individuals and pregnant women mediated inhibition of Fc-rosette formation. Both normal blood B lymphocytes and chronic lymphocytic leukemia cells were used as targets. Inhibition was not related to the presence or absence of lymphocytotoxic antibodies. When tested against a panel of B lymphocytes these sera displayed selective reactivity in keeping with the recognition of allospecific determinants. No association between the target antigen(s) and classically defined MHC-coded structures was evident. Heteroantibodies to both class I and class II MHC structures as well as beta 2-microglobulin also mediated FcR blockade. However, unlike the alloantisera tested, these antibodies displayed no restriction in their reactivity toward individual target cells.
D000910:Antibodies, Heterophile / Q000276:immunology; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology; D001803:Blood Transfusion; D005260:Female; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007165:Immunosuppression Therapy; D007518:Isoantibodies; D007945:Leukemia, Lymphoid / Q000473:pathology; D008297:Male; D011247:Pregnancy; D011961:Receptors, Fc / Q000037:antagonists & inhibitors; D012397:Rosette Formation
D000910:Antibodies, Heterophile; D006680:HLA Antigens; D007518:Isoantibodies; D011961:Receptors, Fc