title: Accumulation of alpha-ketoisocaproate from leucine in homogenates of tissues from rainbow trout (Salmo gairdnerii) and rat. An improved method for determination of branched chain keto acids.
abstract: The dinitrophenyl hydrazone method for determination of branched chain keto acids has been improved by the inclusion of a chromatographic purification step. The dinitrophenyl hydrazone of the keto acid was purified by high pressure liquid chromatography on a reversed phase column, with methanol/acetate buffer as the eluting solvent. Kidney and dark muscle of rainbow trout had very high levels of leucine-alpha-ketoglutarate aminotransferase, while the level was low in liver and white muscle.
mesh_terms: D000818:Animals; D007651:Keto Acids / Q000032:analysis* / Q000096:biosynthesis*; D007668:Kidney / Q000378:metabolism; D007930:Leucine / Q000378:metabolism*; D051276:Leucine Transaminase; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains / Q000378:metabolism; D012487:Salmonidae / Q000378:metabolism*; D013045:Species Specificity; D000637:Transaminases / Q000378:metabolism; D014337:Trout / Q000378:metabolism*
chemical_list: D007651:Keto Acids; C013082:alpha-ketoisocaproic acid; D000637:Transaminases; D051276:Leucine Transaminase; D007930:Leucine