title: Interleukin 1 can replace monocytes for the specific human B-cell response to a particulate antigen.
abstract: It is shown that the anti-trinitrophenyl (TNP) response of human B cells to trinitrophenyl polyacrylamide beads (TNP-PAA) is monocyte dependent. This response is abolished by extensive adherent cell depletion and restored by the addition of monocytes. The optimal response is obtained with 3% monocytes, higher numbers being suppressive. Supernatants from muramyl dipeptide (MDP)-activated monocytes can restore the response of monocyte-depleted preparations even when cells are cultured at suboptimal concentration. A partially purified preparation of interleukin (IL-1) has a comparable restorative ability. The following arguments suggest that monocytes do not function as antigen-presenting cells for this particulate antigen: (i) antigen-pulsed monocytes induce neither an anti-TNP response nor a specific T-cell proliferative response; (ii) allogeneic monocytes function as well as autologous monocytes to restore the response of nonadherent cells; (iii) HLA-DR-negative cells from the human leukemia cell line K562 can replace monocytes for this response. Monocyte supernatants do not replace T cells for the response of B-enriched lymphocytes, showing that T cells are directly involved in B-cell activation.
mesh_terms: D000180:Acrylic Resins / Q000276:immunology*; D000328:Adult; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002460:Cell Line; D002469:Cell Separation; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007375:Interleukin-1 / Q000276:immunology*; D015470:Leukemia, Myeloid, Acute; D008213:Lymphocyte Activation; D009000:Monocytes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D000941:Antigens; D007375:Interleukin-1; C028795:trinitrophenyl polyacrylamide; D000099166:Polyacrylamides