Formation of [3H, 32P]phytic acid in germinating wheat.
Doubly labeled phytic acid of high specific activity was prepared by incubating whole wheat seeds with [32P]phosphoric acid and [3H]myoinositol for 48 h and purifying by anion-exchange chromatography on AG 1- X 8 resin. Both degradation and synthesis of phytic acid were inhibited by KF to a similar extent, yet the catabolic and anabolic pathways involved distinctly different enzyme systems, as no [3H, 32P]myoinositol tetra- or pentaphosphate could be detected.
D002852:Chromatography, Ion Exchange; D007294:Inositol / Q000378:metabolism; D010756:Phosphoric Acids / Q000378:metabolism; D010833:Phytic Acid / Q000032:analysis* / Q000378:metabolism; D014908:Triticum / Q000032:analysis*
D010756:Phosphoric Acids; D007294:Inositol; D010833:Phytic Acid