[Trypsin and chymotrypsin distribution between the fractions of the duodenal contents and the parietal mucosal deposits].
An attempt has been made to assay the distribution of the typical representatives of pancreatic enzymes such as trypsin and chymotrypsin between dense and liquid fractions of the duodenal contents on an empty stomach and parietal mucous overlaps. The mass of the dense fraction being formed and specific activity of the enzymes under study are affected depending on the duodenal contents pH (4.5, 4.0, 3.5). Within the pH ranges indicated, specific activity of trypsin expressed in per cent of the total enzyme activity in the duodenal contents changed from 2-41 to 79-97%. It has been also shown that sorption of the enzymes is possible as well on the structures of the parietal mucous overlaps. A definite correlation has been demonstrated to exist between the activity of the enzymes under study in mucous overlaps and duodenal contents. Thus, the content of two endopeptidases of the pancreatic origin has been measured in the dense fraction of the duodenal contents on an empty stomach and mucous overlaps which are regarded as potential zones of heterophasic digestion in the intestinal cavity.
D000818:Animals; D002918:Chymotrypsin / Q000378:metabolism*; D004063:Digestion; D004285:Dogs; D004386:Duodenum / Q000201:enzymology*; D005215:Fasting; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000201:enzymology*; D007419:Intestinal Secretions / Q000201:enzymology*; D014357:Trypsin / Q000378:metabolism*
D002918:Chymotrypsin; D014357:Trypsin
Raspredelenie tripsina i khimotripsina mezhdu fraktsiiami duodenal'nogo soderzhimogo i pristenochnymi slizistymi nalozheniiami.