Hyperplasia in the spinal sensory system of the frog. I. Plasticity in the most caudal dorsal root ganglion.
Increases in the amount of periphery available for innervation have been achieved by the unilateral removal of hindlimb dorsal root ganglion (DRGs) in Rana pipiens, a procedure which generally results in a compensatory cell number increase (hyperplasia) in the DRGs which remain. We have found that the hyperplastic response is extremely variable, and we have investigated various factors which might control its production. Our findings indicate, however, that the pattern of DRGs removed, the animal's age at the time of removal, and the survival period are not strictly related to the production of hyperplasia in hindlimb DRGs. Special emphasis has been placed on DRG 10, the caudalmost DRG which normally innervates the cloaca and sends a small projection to the hindlimb. This DRG displayed dramatic cell number increases of up to 564%. In addition, several unique features of the hyperplastic response have been observed in DRG 10. This DRG showed increases in cell number on both the operated and the unoperated sides. It showed hyperplasias in animals subjected to ganglionectomy past metamorphosis as well as during larval development. Finally the production of DRG 10 hyperplasias exclusively occurred in male pre- and postmetamorphic animals. To account for these distinctive features of DRG 10 hyperplasia, baseline studies of the normal course of proliferation and cell death in DRG 10 were undertaken. They reveal no fundamental developmental differences between DRG 10 and other hindlimb DRGs. Other mechanisms responsible for these unusual features of developmental plasticity in DRG 10 are discussed.
D000818:Animals; D005260:Female; D005727:Ganglia, Spinal / Q000473:pathology / Q000502:physiology*; D006965:Hyperplasia / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D009473:Neuronal Plasticity; D011894:Rana pipiens / Q000502:physiology*; D012677:Sensation / Q000502:physiology*; D012737:Sex Factors; D013116:Spinal Cord / Q000473:pathology*