The cytotoxic activity of heterologous anti-L-1210 antibodies conjugated with quinones.
Biological effects of conjugates of quinone derivatives with antibodies were studied. Two methods of conjugation of aziridine derivatives of quinone with anti-L-1210 antibodies were used: the direct substitution of the protein amino groups or thiolation of immunoglobulins and subsequent substitution of the sulfhydryl groups introduced. The reactions were carried out in 10% solutions of DMF at pH 8.3-8.9. The biological activity of conjugates, in vitro, was controlled using L-1210 and HeLa cells by measuring the inhibition of incorporation of labeled thymidine. The growth of L-1210 cells was more strongly inhibited by conjugates prepared with anti-L-1210 antibodies than by conjugates with nonimmune IgG. No difference in biological activity was found between conjugates prepared from anti-L-1210 antibodies and normal IgG in tests with HeLa cells.
D000276:Adjuvants, Immunologic; D000818:Animals; D000912:Antibodies, Neoplasm / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006367:HeLa Cells / Q000276:immunology; D007939:Leukemia L1210 / Q000276:immunology*; D051379:Mice; D011809:Quinones / Q000276:immunology* / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D000912:Antibodies, Neoplasm; D011809:Quinones