title: Characterization of human megakaryocyte colony-stimulating factor in the urinary extracts from patients with aplastic anemia and idiopathic thrombocytopenic purpura.
abstract: Using procedures that were effective in the purification of human urinary erythropoietin (Epo), we attempted initial purification of megakaryocyte colony-stimulating factors (CSF) in urinary extracts from patients with aplastic anemia (AA) and idiopathic thrombocytopenic purpura (ITP). Comparison of colony stimulation by purified human Epo and crude urinary extracts revealed: (1) that the pure Epo augments megakaryocyte colony formation in culture and (2) MEG-CSF activity is also present in materials other than Epo in the crude urinary extracts from the two types of patients. Similar to purification of Epo, ethanol precipitation and sulfopropyl-Sephadex chromatography provided twofold and threefold increases in the specific activity of MEG-CSF, respectively. In contrast to Epo, however, significant inactivation of MEG-CSF activity was seen with phenol treatment. The elution profile of MEG-CSF seen on hydroxylapatite chromatography of urinary extracts was different from that of Epo. These data provided a basis for initial steps for purification of MEG-CSF and support the notion that MEG-CSF is distinct from Epo.
mesh_terms: D000636:Aminosalicylic Acids; D000741:Anemia, Aplastic / Q000652:urine*; D002478:Cells, Cultured; D002845:Chromatography; D003115:Colony-Stimulating Factors / Q000302:isolation & purification / Q000652:urine*; D004921:Erythropoietin; D000431:Ethanol / Q000494:pharmacology; D006801:Humans; D006882:Hydroxyapatites; D008533:Megakaryocytes / Q000166:cytology*; D011696:Purpura, Thrombocytopenic / Q000652:urine*
chemical_list: D000636:Aminosalicylic Acids; D003115:Colony-Stimulating Factors; D006882:Hydroxyapatites; D004921:Erythropoietin; D000431:Ethanol; C037254:phenyl 4-aminosalicylate