Analysis of 5-pyrrolidone-2-carboxylate ester by reverse phase high-performance liquid chromatography.
A simple quantitative method for estimating nanomole concentrations of 5-pyrrolidone-2-carboxylic acid (PCA) in tissue homogenates from mouse has been developed using reverse-phase HPLC. PCA was detected as the 4-nitrophenacyl ester which has an absorption maximum at 263 nm, a relatively high stability, and excellent chromatographic separation and detectability. This method offers distinct advantages over other analytical procedures thus far employed for measuring PCA in that the 4-nitrophenacyl derivative of PCA can be readily prepared from deproteinized tissue homogenates and quantitated by HPLC within relatively short time intervals with good precision and specificity.
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D008297:Male; D051379:Mice; D009578:Nitrobenzenes / Q000032:analysis; D011760:Pyrrolidinones / Q000032:analysis*; D011761:Pyrrolidonecarboxylic Acid / Q000032:analysis*; D012867:Skin / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
D009578:Nitrobenzenes; D011760:Pyrrolidinones; D011761:Pyrrolidonecarboxylic Acid