title: Serological response to rubella revaccination in adolescent females.
abstract: The study's purpose was to utilize ELISA in determining rubella susceptibility in teenagers who are seronegative by HI. Ten previously vaccinated, seronegative adolescent females were revaccinated with RA 27/3 and their IgG and IgM responses measured by ELISA. Before revaccination, all 10 were seronegative for rubella by HI (less than 1:10). However, by ELISA, six showed an IgG and no IgM levels. After reimmunization, three showed a significant IgM response by day 6, indicating primary rubella exposure. The remaining seven females failed to demonstrate an IgM response; however, the failure was consistent with a history of prior rubella vaccination. Peak IgG responses were noted by day 12 in nine of the females. The remaining teenager failed to show an IgG response and was considered a vaccine failure. This study confirms the superiority of ELISA as compared to HI titers in determining rubella susceptibility.
mesh_terms: D000293:Adolescent; D000914:Antibodies, Viral / Q000032:analysis*; D004198:Disease Susceptibility; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D012409:Rubella / Q000276:immunology / Q000517:prevention & control*; D012411:Rubella Vaccine / Q000008:administration & dosage*; D014611:Vaccination
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012411:Rubella Vaccine