title: Folding of homologous proteins. The refolding of different ribonucleases is independent of sequence variations, proline content and glycosylation.
abstract: The refolding kinetics of four different pancreatic ribonucleases have been compared. Bovine and ovine RNAase contain 4 proline residues, red deer RNAase has 5 prolines, the enzyme from roe deer 6 prolines. Despite the variation in the amount of prolines, all four proteins show a constant value of 20% fast refolding species UF. The extra proline residues of the deer enzymes do not increase the amount of slow refolding species US. Consequently these residues may be non-essential for folding. Despite many differences in the amino acid sequence, the rates if the fast and slow refolding reactions are very similar for all investigated ribonucleases. This indicates that the pathway of refolding has been conserved during evolution, i.e. the positions where amino acid substitutions occur are not critically important for the rate-determining steps of the folding process. A carbohydrate chain attached to ribonuclease does not alter the folding properties of the protein: RNAase A and RNAase B from roe deer show identical refolding kinetics.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002241:Carbohydrates; D002417:Cattle; D003568:Cytidine Monophosphate / Q000494:pharmacology; D003670:Deer; D007700:Kinetics; D011392:Proline / Q000032:analysis; D011487:Protein Conformation; D012259:Ribonuclease, Pancreatic / Q000037:antagonists & inhibitors; D012260:Ribonucleases / Q000037:antagonists & inhibitors; D012756:Sheep; D013053:Spectrophotometry; D013696:Temperature; D014443:Tyrosine
chemical_list: D002241:Carbohydrates; D014443:Tyrosine; D011392:Proline; D012260:Ribonucleases; C029600:ribonuclease B; D012259:Ribonuclease, Pancreatic; D003568:Cytidine Monophosphate