An evaluation of the dorset sheep as a predictive animal model for the response of G-6-PD deficient human erythrocytes to a proposed systemic toxic ozone intermediate, methyl oleate hydroperoxide.
Erythrocytes of both G-6-PD deficient humans and Dorset sheep, an animal model with an erythrocyte G-6-PD deficiency, both responded in a dose dependent manner to the oxidant stress of MOHP as measured by decreases in G-6-PD activity, increases in METHB levels and decreases in GSH. However, the human G-6-PD deficient erythrocytes were considerably more sensitive to the formation of METHB than the sheep erythrocytes while the reverse was the case for the GSH parameter. The results suggest a qualitative difference in the response of sheep erythrocytes and human G-6-PD deficient erythrocytes to MOHP that seriously questions the value of the sheep erythrocyte as a quantitatively accurate predictive model.
D000818:Animals; D004195:Disease Models, Animal; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D005260:Female; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000097:blood*; D005978:Glutathione / Q000032:analysis; D006801:Humans; D008054:Lipid Peroxides / Q000633:toxicity*; D008706:Methemoglobin / Q000032:analysis; D010126:Ozone / Q000633:toxicity*; D012756:Sheep / Q000097:blood*; D013045:Species Specificity
D008054:Lipid Peroxides; C037581:methyl oleate hydroperoxide; D010126:Ozone; D008706:Methemoglobin; D005978:Glutathione