The use of thiol-disulphide exchange chromatography for the automated isolation of alpha 1-antitrypsin and other plasma proteins with reactive thiol groups.
A method has been developed for the rapid isolation of alpha 1-antitrypsin and other thiol proteins from plasma by an automated chromatography system. The thiol-proteins are initially bound to matrix-linked activated thiol-compounds by an SH-SS interchange reaction. The mixed disulphides are then reduced in two steps and subfractionated by passage through Blue-Sepharose and AH-Sepharose columns. The rate of the interchange reactions varies with the microenvironment of the reacting thiols. alpha 1-Antitrypsin is recovered with 95% purity in 60% yield within two days from 1 l of plasma.
D001798:Blood Proteins / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange / Q000379:methods; D004220:Disulfides; D006801:Humans; D008970:Molecular Weight; D013438:Sulfhydryl Compounds / Q000097:blood* / Q000302:isolation & purification; D000515:alpha 1-Antitrypsin / Q000302:isolation & purification*
D001798:Blood Proteins; D004220:Disulfides; D013438:Sulfhydryl Compounds; D000515:alpha 1-Antitrypsin