Chromatographic analysis of blood lipids. Comparison between gas chromatography and thin-layer chromatography with flame ionization detection.
Intact human blood plasma lipids of different composition were analyzed by gas chromatography and thin-layer chromatography with flame ionization detection. The reproducibility of the results obtained by gas and thin-layer chromatography was compared. The main advantages and disadvantages of both methods for lipid analysis are discussed. Generally, the variability of the results measured by thin-layer chromatography in series and from day to day was greater than that obtained by gas chromatography.
D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005410:Flame Ionization; D006801:Humans; D008055:Lipids / Q000097:blood* / Q000302:isolation & purification; D010713:Phosphatidylcholines / Q000097:blood; D010714:Phosphatidylethanolamines / Q000097:blood; D010716:Phosphatidylinositols / Q000097:blood; D010718:Phosphatidylserines / Q000097:blood; D013109:Sphingomyelins / Q000097:blood
D008055:Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D010718:Phosphatidylserines; D013109:Sphingomyelins