Enzyme-linked immunosorbent assay for determination of HLA: gene dose effect.
In a previous publication we demonstrated that polymorphic HLA antigens could be detected on fresh and dried peripheral blood lymphocytes using the enzyme-linked immunosorbent assay (ELISA) with HLA alloantisera (Bishara et al. 1983). In the present study we investigated whether the ELISA technique can be used in determination of gene-dose effect for antigens of the HLA-A and B loci. Lymphocytes from HLA-A1 and HLA-B14 homozygous individuals are shown to bind significantly more anti-HLA alloantibodies than their heterozygous siblings for the same HLA antigens. These results indicate that ELISA is a sensitive and reliable technique for the qualitative and quantitative assay of polymorphic HLA determinants.
D000328:Adult; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006680:HLA Antigens / Q000032:analysis* / Q000235:genetics; D015788:HLA-A1 Antigen; D015235:HLA-B Antigens; D059909:HLA-B14 Antigen; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D011110:Polymorphism, Genetic
D006680:HLA Antigens; D015788:HLA-A1 Antigen; D015235:HLA-B Antigens; D059909:HLA-B14 Antigen