Transfer of [3H]pyrrolizidine alkaloids from Senecio vulgaris L. and metabolites into rat milk and tissues.
[3H]Retronecine and [3H]necic acid-labelled senecionine and seneciphylline were prepared biosynthetically with seedlings of Senecio vulgaris L. using [2,3-3H]putrescine and [4,5-3H]isoleucine, respectively, as precursors. Lactating rats dosed with these differently labelled pyrrolizidine alkaloids (PAs) excreted within 3 h approx. 0.08% of the applied radioactivity in the milk mainly as yet not identified water-soluble retronecine-derived metabolites and with approx. 0.02% as unchanged PAs. Highest tissue levels of PAs and metabolites, 6 h after administration, were found in liver and lungs.
D000818:Animals; D003998:Dicarboxylic Acids / Q000378:metabolism; D005260:Female; D007700:Kinetics; D008892:Milk / Q000378:metabolism*; D010947:Plants, Toxic; D011247:Pregnancy; D011763:Pyrrolizidine Alkaloids / Q000378:metabolism*; D051381:Rats; D012674:Senecio; D014018:Tissue Distribution; D014316:Tritium
D003998:Dicarboxylic Acids; D011763:Pyrrolizidine Alkaloids; C013678:seneciphylline; D014316:Tritium; C008590:necic acid; C014795:retronecine; C009237:senecionine