Preparation of a low-density species of endocytic vesicle containing immunoglobulin A.
Immunoglobulin A is transported across hepatocytes in specialized vesicles. A population of endocytic vesicles of approx. 140 nm diameter, containing immunoglobulin A, has now been separated from all other major cytoplasmic organelles, including plasma membrane and lysosomes, by sequential centrifugation on Ficoll/sucrose and Metrizamide gradients.
D000818:Animals; D001692:Biological Transport; D002500:Centrifugation, Isopycnic; D004705:Endocytosis; D007070:Immunoglobulin A / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000276:immunology*; D008297:Male; D008854:Microscopy, Electron; D009940:Organoids / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains
D007070:Immunoglobulin A