T-lymphocyte activation by a gluten fraction, glyc-gli. Studies of adult coeliac patients and healthy controls.
A gluten fraction (glyc-gli) induced proliferation of T lymphocytes obtained from coeliac patients or healthy controls. Glyc-gli acted as an antigen, and T-cell activation was strongly dependent on antigen-presenting monocytes in the cultures. The lymphocyte response was similar for HLA-B8/DR3-positive and HLA-B8/DR3-negative healthy controls, and coeliac patients in remission on a gluten-free diet could not be distinguished from the controls by the degree of proliferative activity in this test. Conversely, untreated coeliac patients showed a significantly lower lymphocyte response to glyc-gli than both coeliac patients in remission and controls. This result may reflect sequestration of gluten-specific cells into the mucosal lesion or mesenteric lymph nodes in patients with active coeliac disease.
D000293:Adolescent; D000328:Adult; D000368:Aged; D002446:Celiac Disease / Q000178:diet therapy / Q000235:genetics / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005983:Glutens / Q000008:administration & dosage / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology; D015795:HLA-B8 Antigen; D015803:HLA-DR3 Antigen; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D006680:HLA Antigens; D015795:HLA-B8 Antigen; D015803:HLA-DR3 Antigen; D000949:Histocompatibility Antigens Class II; D005983:Glutens