Posttranslational modification of human T-cell growth factor.
Amino-terminal sequence analysis of human T-cell growth factor indicated that the amino acid in position 3 of the polypeptide chain was modified. Examination of the N-terminal octapeptide using the amino acid analyzer and mass spectrometry demonstrated that position 3 was a threonine which was linked to N-acetyl-D-galactosamine. This site of glycosylation is of practical significance since it appears to play a role in the selectivity of a monoclonal antibody for the factor.
D000595:Amino Acid Sequence; D002460:Cell Line; D006023:Glycoproteins / Q000235:genetics; D006595:Hexosamines / Q000032:analysis; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics*; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000032:analysis; D011499:Protein Processing, Post-Translational; D013601:T-Lymphocytes; D014357:Trypsin
D006023:Glycoproteins; D006595:Hexosamines; D007376:Interleukin-2; D010446:Peptide Fragments; D014357:Trypsin