Biochemical studies on structural and nonstructural proteins of the African green monkey B-lymphotropic papovavirus (LPV).
African green monkey (AGM) B-lymphotropic papovavirus (LPV) particles were separated from infected B-lymphoma BJA-B cells by neuraminidase and deoxycholate treatment and were further purified twice by CsCl density centrifugation. The SDS-PAGE analysis of virus particles banding at a density of 1.3510 g/ml and radioactively labeled by 125I revealed a major polypeptide of 40,000 and two minor polypeptides of 42,000 and 29,000. In addition, in infected BJA-B cells, all three viral structural polypeptides could be identified by immunoprecipitation. A nonstructural phosphopolypeptide of approximately 90,000 MW could be detected when sera against SDS-denatured SV40 T antigen or an AGM serum pool were used for immunoprecipitation. It is suggested that the 90K polypeptide of LPV represents an equivalent to T antigens of other papovaviruses.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D000957:Antigens, Viral, Tumor; D001402:B-Lymphocytes / Q000382:microbiology; D002460:Cell Line; D002522:Chlorocebus aethiops; D006801:Humans; D008970:Molecular Weight; D027383:Papillomaviridae / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D027382:Polyomaviridae; D014764:Viral Proteins / Q000032:analysis*; D015678:Viral Structural Proteins
D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor; D014764:Viral Proteins; D015678:Viral Structural Proteins