title: Analysis of surface saccharides in Trichomonas vaginalis strains with various pathogenicity levels by fluorescein-conjugated plant lectins.
abstract: Certain surface saccharides of organisms from clone-derived cultures of five Trichomonas vaginalis strains, JH30A-cl. 1, JH31A-cl. 1, JH32A-cl. 1, JH34A-cl. 1, JH162A-cl. 1, and JH384A-cl. 2, which differed in their pathogenicity for women and experimental hosts, were compared with the aid of fluorescein-conjugated plant lectins using a quantitative fluorescence method. The lectins used were: concanavalin A (Con-A), wheat germ agglutinin (WGA), soybean agglutinin (SBA), castor bean agglutinin (CBA), and garden pea agglutinin (GPA). On the basis of experimental results and control experiments, the latter involving incorporation of specific inhibitory sugars in the reaction mixtures, it was concluded that: (1) All five strains had large numbers of Con-A- and WGA-binding saccharide residues. (2) Some also had smaller numbers of SBA- and CBA-binding sites. (3) No strain bound significant amounts of GPA. The differences in CBA binding were not related to pathogenicity of the parasites; however, those in SBA binding could be correlated with the pathogenicity levels of the five strains. The results obtained with SBA in the presence of N-acetyl-D-galactosamine and D-lactose and those recorded for GPA suggested that the differences between the pathogenic and mild T. vaginalis strains reflected the levels of D-lactosyl residues on the cell surfaces--these residues were more abundant on strains having higher pathogenicity levels. Possible explanation of the apparent relationships between the presence of the specific sugar residues and pathogenicity are suggested directly or by analogy with other pyranosyls (galactosyls).
mesh_terms: D000818:Animals; D001665:Binding Sites; D002241:Carbohydrates / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D003208:Concanavalin A; D005260:Female; D006801:Humans; D037102:Lectins; D051379:Mice; D037121:Plant Lectins; D030262:Soybean Proteins; D014246:Trichomonas vaginalis / Q000032:analysis* / Q000472:pathogenicity; D014909:Wheat Germ Agglutinins; D010835:Phytohemagglutinins
chemical_list: D002241:Carbohydrates; D003208:Concanavalin A; D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins; C017707:Ricinus communis agglutinin-1; C028824:soybean lectin; D010835:Phytohemagglutinins