Analysis of normal and neoplastic lymphocyte surface-labeled proteins by two-dimensional polyacrylamide gel electrophoresis.
Normal and neoplastic murine and human lymphocytes were surface-labeled by lactoperoxidase-catalyzed radioiodination, and the cell lysates were subjected to two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) analyses, combining isoelectric focusing in the first dimension and sodium dodecyl sulfate-PAGE (SDS-PAGE) in the second dimension. 2D-PAGE autoradiogram patterns were reproducible and reflected differences in cell types. A string of spots with a Mr of 100K was tentatively identified as a new T-cell marker (Tp100) which was present in all murine and human T cells examined including human T lymphomas. Murine and human B cells displayed markers characteristic to B cells of each species with some similarities between them. Human lymphomas and murine cell lines showed markers which were absent or only weakly visible in normal cells. Thus, 2D-PAGE analysis of lymphocyte surface proteins proved to be a method useful for searching for various markers.
D000818:Animals; D001402:B-Lymphocytes / Q000032:analysis*; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D006023:Glycoproteins / Q000097:blood; D006801:Humans; D007525:Isoelectric Focusing; D008223:Lymphoma / Q000097:blood*; D008297:Male; D008565:Membrane Proteins / Q000097:blood*; D051379:Mice; D013601:T-Lymphocytes / Q000032:analysis*
D006023:Glycoproteins; D008565:Membrane Proteins