Inhibition of the elongation step of protein synthesis by vaccinia virus.
Vaccinia cores inhibit translation in cell-free protein synthesis systems at two stages: initiation; and, as shown here, elongation. The former effect tends to obscure the latter. Elongation control could, however, be revealed as follows: when, in a reticulocyte of L-cell lysate, initiation was blocked by a drug (edein), the residual [35S]methionine incorporation was severely reduced by the subsequent addition of vaccinia cores. The elongation block could also be demonstrated by analysis of ribosome profiles: treatment with edein alone permitted ribosomal run-off; treatment with either the elongation inhibition anisomycin or with cores preserved the polyribosomes.
D000818:Animals; D002474:Cell-Free System; D004486:Edeine / Q000494:pharmacology; D007700:Kinetics; D007739:L Cells / Q000378:metabolism; D051379:Mice; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects; D014176:Protein Biosynthesis / Q000187:drug effects; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D014616:Vaccinia virus / Q000187:drug effects / Q000235:genetics*
D004486:Edeine