G6PD isoenzyme analysis of myeloid and lymphoid cells in human multilineage colonies.
Some multilineage hemopoietic colonies contain, in addition to myeloid cells, T lymphocytes. These proliferate extensively in liquid suspension culture under the influence of a T cell growth factor provided by phytohemagglutinin-T cell-conditioned medium (PHA-TCM). The clonal origin of these myeloid and lymphoid components was investigated by determining the glucose-6-phosphate dehydrogenase (G6PD) isoenzyme types of multilineage colonies grown from peripheral blood of 4 G6PD heterozygous normal volunteers. The G6PD assay is sufficiently sensitive to detect enzyme concentrations contributed by as few as 30 granulocytes and erythroblasts, 4-6 megakaryocytes, 2-3 macrophages, and 50-100 T cells. T cell components can be detected even if myeloid cells are present in 10-20-fold excess. A small number of multilineage colonies with T cells produced a single G6PD isoenzyme on direct analysis and after expansion in liquid culture. This observation supports the view of a common progenitor for myeloid and lymphoid cells in the peripheral blood of normal adults.
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D002478:Cells, Cultured; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000201:enzymology; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D007962:Leukocytes / Q000201:enzymology*; D008214:Lymphocytes / Q000201:enzymology*; D013223:Statistics as Topic; D013601:T-Lymphocytes / Q000145:classification
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D005954:Glucosephosphate Dehydrogenase