Thin layer gel filtration immunofixation: identification of abnormal molecular weight immunoglobulins or related fragments.
A simple procedure is described to identify immunoglobulin (Ig) fragments without isolation from biological fluids. It involves an initial separation based on molecular weight (MW) by thin layer gel filtration (TLG) followed by immunofixation (IF) in cellulose acetate strips with monovalent antisera. TLG-IF permits detection of differences about 10,000 Da MW and specific immunological typing, making it a useful tool in the accurate identification of protein fragments.
D000888:Antibodies, Anti-Idiotypic; D002850:Chromatography, Gel / Q000379:methods; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D006362:Heavy Chain Disease / Q000276:immunology*; D006801:Humans; D007128:Immunoglobulin Fragments / Q000302:isolation & purification*; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification; D008970:Molecular Weight
D000888:Antibodies, Anti-Idiotypic; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G