title: [Effect of the content of plant crude protein in the ration on the utilization of urea by the milk cow. 1. Nitrogen digestibility and utilization of urea for bacterial protein synthesis in the rumen].
abstract: The utilisation of feed urea in the rumen was tested in 2 experiments with a total of 4 newly lactating dairy cows (13 . . . 15 and 17 . . . 19 kg resp. milk/animal and day) with rumen and duodenal re-entrant cannulae. With the energy supply remaining constant in each case, the rations in experiment A contained 8.7, 12.4 and 14.6 and those of experiment B 10.7, 13,7 and 17.1% crude plant protein in the dry matter. After the supplementation with 120 and 150 g resp. urea/animal and day there were 11.9, 15.7 and 17.8 (A) and 13.8, 16.7 and 20.2 (B) % resp. crude protein in the dry matter. The rations consisted of maize silage and a pelleted mixture of straw and concentrated feed (A) resp. maize silage, alfalfa hay and concentrated feed (B). They contained 10.3 . . . 10.6 (A) and 13.6 (B) kg dry matter with 5.6 . . . 6.0 (A) and 8.2 (B). With the increase of the crude protein level of the ration to 16.7 . . . 17.8, the absolute amount of non-NH3-N (NAN) in the duodenum increased as well. Between N-intake (g/d, x) and NAN-passage corrected by the amount of the endogenous quota (g/d, y) the relation y = 87.3 + 0.55 x (r = 0.80) could be established. NAN-passage (y) as related to N-intake decreased with the N-concentration in the dry matter of the ration (x) according to the equation y = 0.35 + 1.22x-1 (r = 0.57). 70, 62 and 61% (experiment A) and 55, 61 and 51% (experiment B) of the consumed amount of N were apparently absorbed in the intestines as NAN (without endogenous quota). The bacterial N-yield of the rumen (g, y), determined with diamino pimelic acid as microbe marker, was dependent on the consumed digestible organic matter (g, x) as follows: y = 67.3 + 0.021x (r = 0.69). There was no connection with the level of N-supply. The measuring results of the bacterial N-yield show that the utilisation rate of the urea-N decreased rapidly when there was more than 11 . . . 12% crude plant protein in the dry matter of the ration. For the tested ration type (570 . . . 600 EFUcattle/kg dry matter) the urea utilisation potential in the rumen for crude plant protein concentrations of 8.7, 10.7, 12.4, 13.4, 13.7, 14.6 and 17.1% in the dry matter was 13.0, 6.9, 4.1, 2.6, 1.3 and -9.6 g urea/kg dry matter.
mesh_terms: D000821:Animal Feed; D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis; D002417:Cattle; D004044:Dietary Proteins / Q000378:metabolism*; D004386:Duodenum / Q000378:metabolism; D004734:Energy Metabolism; D005260:Female; D007700:Kinetics; D009584:Nitrogen / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D012417:Rumen / Q000382:microbiology*; D014508:Urea / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004044:Dietary Proteins; D010940:Plant Proteins; D014508:Urea; D009584:Nitrogen
vernacular_title: Untersuchungen zum Einfluss des Gehaltes an pflanzlichem Roh-protein in der Ration auf die Verwertung von Harnstoff bei der Milchkuh. 1. Mitteilung. Stickstoffverdauung und Verwertung des Harnstoffs für die bakterielle Proteinsynthese im Pansen.