The glycoprotein nature of solubilized muscarinic acetylcholine receptors from bovine cerebral cortex.
Muscarinic acetylcholine receptors were solubilized from bovine cerebral cortex with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate. The so-obtained receptors could be precipitated by Wheat Germ (73%), Concanavalin A (55%), Lens Culinaris (36%) and Ricinus Communis (26%), but not by Peanut, Dolichus Biflorus and Ulex Europaeus. On Wheat Germ- and Concanavalin A-affinity chromatography, the solubilized muscarinic receptors were retained on both columns and subsequently eluted with N-acetylglucosamine and alpha-methyl-D-mannoside, respectively. A high concentration (100 micrograms/ml) of Wheat Germ or Concanavalin A did not interfere with Z-[3H]quinuclidinyl benzilate binding, thereby suggesting that the lectin binding sites are not directly involved in the receptor binding function. These solubilized muscarinic receptors are postulated to contain carbohydrate residues, N-acetyl-glucosamine, mannose and galactose, as glycoprotein.
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002540:Cerebral Cortex / Q000378:metabolism*; D002846:Chromatography, Affinity / Q000379:methods; D006023:Glycoproteins / Q000302:isolation & purification*; D037102:Lectins; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000302:isolation & purification* / Q000378:metabolism; D012995:Solubility; D013329:Structure-Activity Relationship; D013570:Synaptic Membranes / Q000378:metabolism*
D006023:Glycoproteins; D037102:Lectins; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate; D000109:Acetylcholine