An inexpensive, microcomputer-based, video densitometer for quantitating thin-layer chromatographic spots.
An inexpensive video densitometer based on an Apple II microcomputer using commercially available components was developed. The device was used to quantitate charred lipid and colored spots on thin-layer chromatography plates. The method is useful for lipid samples weighing 0.1 to 4 micrograms on high-performance plates and 1 to 20 micrograms on ordinary plates. The software automatically corrects for baseline drift due to dirt or nonuniformity in the plate.
D002518:Ceramides / Q000032:analysis; D002784:Cholesterol / Q000032:analysis; D002855:Chromatography, Thin Layer / Q000295:instrumentation* / Q000379:methods; D003201:Computers / Q000191:economics; D003720:Densitometry / Q000295:instrumentation*; D008838:Microcomputers / Q000191:economics
D002518:Ceramides; D002784:Cholesterol