Immunocytochemical localization of cathepsin B in degenerating rat skeletal muscle induced by a local anesthetic, bupivacaine.
Experimental acute degenerative changes in skeletal muscle accompanied by a drastic increase in cathepsins B&L were induced in rats by intramuscular injection of a local anesthetic, bupivacaine. Cathepsins B&L have been implicated in the rapid disappearance of muscle fibers. Degenerating muscle showed a spotty fluorescence when stained with antibodies against cathepsin B, indicating that the increased cathepsin B did not originate from the muscle itself, but from invading phagocytes. We report here results showing that cathepsin B of nonmuscle cell origin is involved in the breakdown of myofibrillar proteins in acute bupivacaine-induced muscle degeneration.
D000818:Animals; D002045:Bupivacaine; D002401:Cathepsin B; D002403:Cathepsins / Q000032:analysis*; D006651:Histocytochemistry; D007120:Immunochemistry; D009135:Muscular Diseases / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D010586:Phagocytes / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
D002403:Cathepsins; D002401:Cathepsin B; D002045:Bupivacaine