The effectiveness of 3H-uridine and 3H-leucine for labeling of T and B cells of rats and mice.
Using a technique for combined autoradiography and immunoperoxidase staining, we studied the incorporation of 3H-Uridine and 3H-Leucine into T and B lymphocytes of rats and mice. While in mouse both T and B cells were clearly labeled by incorporation of 3H-Uridine, a portion of rat T cells remained unlabeled. Furthermore, the majority of rat B cells was not labeled. In contrast, mouse T and B cells as well as rat T and B cells could be labeled with 3H-Leucine to a comparable degree.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D007930:Leucine / Q000378:metabolism*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D014316:Tritium / Q000378:metabolism; D014529:Uridine / Q000378:metabolism*
D014316:Tritium; D007930:Leucine; D014529:Uridine