title: Cytotoxic effect of vanadium and oil-fired fly ash on hamster tracheal epithelium.
abstract: Hamster tracheal organ cultures were used to study the in vitro effects of vanadium and oil-fired fly ash on mucociliary respiratory epithelium. Two vanadium compounds, VOSO4 and V2O5, and fly ash from an oil-fueled power plant were dissolved or suspended in culture medium over a range of concentrations and epithelia were exposed for 1 hr/day, for 9 consecutive days. At intervals during this period, alterations in cilia-beating frequency, cytology, and histology were documented by light microscopy. Explants treated with VOSO4 either decreased ciliary activity or produced ciliostasis depending upon the concentration and length of exposure. Early morphological alterations consisted of vacuolization of both nuclei and cytoplasm. After multiple exposures, cytology of VOSO4-treated respiratory mucosa was markedly affected. Similar changes were observed in cultures exposed to V2O5; however, the cytotoxicity appeared earlier and was more pronounced. Fly ash-treated explants produced similar biological effects when compared to both vanadium compounds. Thus, the data indicate that the extent of vanadium toxicity depends, at least in part, on the vanadium content of the compound tested, and that exposure to this metal and vanadium-rich fly ash can inhibit normal mucociliary function, a vital clearance mechanism in the respiratory tract.
mesh_terms: D000818:Animals; D002923:Cilia / Q000187:drug effects; D006224:Cricetinae; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D066298:In Vitro Techniques; D008297:Male; D008647:Mesocricetus; D010578:Petroleum / Q000633:toxicity; D014132:Trachea / Q000187:drug effects* / Q000473:pathology; D014639:Vanadium / Q000633:toxicity*; D017968:Vanadium Compounds
chemical_list: D010578:Petroleum; D017968:Vanadium Compounds; D014639:Vanadium; C034028:vanadyl sulfate