Modulation of the biologic activities of IgE-binding factor. II. Physicochemical properties and cell sources of glycosylation-enhancing factor.
T lymphocytes of rats treated with Bordetella pertussis vaccine (BP) formed a soluble factor that enhanced the glycosylation of IgE-binding factors during their biosynthesis, and provided the latter factors with the biologic activity to potentiate the IgE response. The present experiments demonstrated that pertussigen (leukocytosis-promoting factor) from BP induced normal rat spleen cells to form the glycosylation-enhancing factor. The same factor was obtained by incubation of normal spleen cells with 5 micrograms/ml, but not 2 micrograms/ml, concanavalin A. When normal rat mesenteric lymph node cells were incubated with the glycosylation-enhancing factor together with IgE, IgE-binding factors formed by the cells selectively potentiated the IgE response. The IgE-binding factors formed by the same cells upon incubation with IgE alone neither enhanced nor suppressed the IgE response. The glycosylation-enhancing factor changed the nature of IgE-binding factors formed by the rat-mouse T cell hybridoma, 23A4. IgE-binding factors induced by IgE alone lacked affinity for lentil lectin, whereas those induced by IgE in the presence of the glycosylation-enhancing factor had affinity for the lectin. The cell source of the glycosylation-enhancing factor appeared to be W 3/25+ Fc gamma R+ T cells. The glycosylation-enhancing factor was protein in nature and had a m.w. of about 25,000. The factor had affinity for acid-treated Sepharose and could be recovered from the beads by elution with lactose. The factor was different from interleukin 2 with respect to both its affinity for galactose and its isoelectric point.
D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism* / Q000502:physiology; D002448:Cell Adhesion; D002469:Cell Separation; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003208:Concanavalin A / Q000494:pharmacology; D006027:Glycosides / Q000378:metabolism*; D007073:Immunoglobulin E; D007376:Interleukin-2; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000502:physiology*; D037342:Pertussis Toxin; D051381:Rats; D011917:Rats, Inbred Lew; D011961:Receptors, Fc / Q000032:analysis; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D010566:Virulence Factors, Bordetella
D001427:Bacterial Toxins; D006027:Glycosides; D007376:Interleukin-2; D008222:Lymphokines; D011961:Receptors, Fc; D010566:Virulence Factors, Bordetella; D003208:Concanavalin A; D007073:Immunoglobulin E; D037342:Pertussis Toxin