[In vitro maturation of the oocytes of the common frog with and without follicular envelopes as affected by cholesterol].
Cholesterol was shown to induce in vitro maturation of the Rana temporaria oocytes both in the presence and in the absence of the follicle cells. The maturation of the denuded oocytes required their much shorter treatment with cholesterol. Ethidium bromide inhibited the cholesterol-induced maturation of the oocytes with follicle envelopes and either did not affect the oocyte maturation after the follicle envelope removal or stimulated it. Amino-gluthetimide inhibited the cholesterol-induced oocyte maturation irrespective of the presence of follicle cells. The mechanism inducing effect of cholesterol on follicle and oocyte is discussed.
D000616:Aminoglutethimide / Q000494:pharmacology; D000818:Animals; D002784:Cholesterol / Q000494:pharmacology*; D004996:Ethidium / Q000494:pharmacology; D005260:Female; D007552:Isotonic Solutions; D009865:Oocytes / Q000187:drug effects*; D010063:Ovum / Q000187:drug effects*; D011896:Rana temporaria; D000077331:Ringer's Solution; D013997:Time Factors; D015044:Zona Pellucida / Q000187:drug effects*
D007552:Isotonic Solutions; D000616:Aminoglutethimide; D000077331:Ringer's Solution; D002784:Cholesterol; D004996:Ethidium
Sozrevanie in vitro ootsitov travianoĭ liagushki, odetykh follikuliarnymi obolochkami i lishennykh ikh pod deĭstviem kholesterina.