title: Intracellular chloride activity and membrane potential in stripped frog skin (Rana temporaria).
abstract: The regulation of cell chloride activity in frog skin was investigated using double barrelled Cl--microelectrodes to measure cell membrane potentials and chloride activity in the isolated frog epidermis. Experiments were done under short-circuit conditions, impaling cells from the serosal side. The basic electrophysiological parameters of the isolated skin were similar to those reported in the literature for whole preparations. Intracellular chloride activity was on average 21.9 mM and membrane potential was about 57 mV, implying that chloride was distributed away from its electrochemical equilibrium (i.e., concentrated inside the cells). Chloride activity decreased after removal of either Cl- or Na+ from the serosal bathing solution, with no change in membrane potential. The chloride permeability of the serosal membrane was calculated to be 2.6 X 10(-6) cm X s-1 which represents about 1/4 of the total conductance of the serosal membrane. We suggest that an electrically silent sodium-dependent uphill transport of chloride is present at the basolateral membrane of the frog skin, which accounts for the non-passive distribution of chloride.
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000032:analysis*; D002712:Chlorides / Q000032:analysis* / Q000378:metabolism; D004594:Electrophysiology; D007424:Intracellular Fluid / Q000032:analysis*; D008564:Membrane Potentials; D008839:Microelectrodes; D011896:Rana temporaria; D012879:Skin Physiological Phenomena; D012964:Sodium / Q000378:metabolism
chemical_list: D002712:Chlorides; D012964:Sodium