Structural homology of corticotropin-releasing factor, sauvagine, and urotensin I: circular dichroism and prediction studies.
Three recently isolated peptides, whose sequences have been determined--the corticotropin (adrenocorticotropic hormone)-releasing factor of ovine origin, sauvagine, from the skin of the frog Phyllomedusa sauvagei, and urotensin I from the teleost fish, Catostomus commersoni--show high (greater than 50%) sequence homology. CD spectra of the three peptides in trifluoroethanol indicate predominantly helical character for these peptides. Analysis of the secondary structures by the Chou-Fasman method predicts that the overall structural organization of the peptides is the same. All three possess a long internal helix, spanning about 25 residues, connected by a turn region to a COOH-terminal structural element that is an alpha-helix in corticotropin-releasing factor and urotensin I and a beta-sheet in sauvagine. The values for helical content estimated from the prediction method agree reasonably well with those computed from the CD spectra. This agreement as well as the CD spectra of corticotropin-releasing factor fragment 5-33 support the specific assignments of helical regions derived from the Chou-Fasman analysis. The three peptides exhibit significantly less helical structure in water than in trifluoroethanol as indicated by CD spectra. Hydrophilicity profiles provided comparison of the three peptides in terms of their overall hydrophilicity and the location of the regions of maximal hydrophilicity. A unique distribution of hydrophilic and hydrophobic residues within the internal helices is revealed by helical wheel analysis. Patches of both types of residues are formed following a heptad (four/three) rule. Since the two patches are shifted by one residue relative to one another, together they occupy only one face of the helical surface, a feature distinct from other amphiphilic structures.
D029845:Amphibian Proteins; D000818:Animals; D001001:Anura; D002942:Circular Dichroism; D003346:Corticotropin-Releasing Hormone; D005399:Fishes; D008958:Models, Molecular; D010446:Peptide Fragments / Q000032:analysis; D036361:Peptide Hormones; D010455:Peptides; D011487:Protein Conformation; D012756:Sheep; D012867:Skin; D013329:Structure-Activity Relationship; D014579:Urotensins; D014665:Vasodilator Agents
D029845:Amphibian Proteins; D010446:Peptide Fragments; D036361:Peptide Hormones; D010455:Peptides; D014579:Urotensins; D014665:Vasodilator Agents; C025727:sauvagine; D003346:Corticotropin-Releasing Hormone; C029676:urotensin I