Preparation of membrane fraction from herpes simplex virus-infected cells which induce cytotoxic T lymphocytes.
The immunogenic capacity of herpes simplex virus (HSV)-infected cells and their subcellular membrane fractions was investigated by assessing the anti-HSV cytotoxic T lymphocyte (CTL) response in cultures of spleen lymphocytes from HSV-primed BALB/c mice. Methylchloranthrane-induced fibrosarcoma (Meth A) cells infected with HSV (HSV-Meth A) were fixed either with glutaraldehyde or by heating at 56 C to preserve their immunogenic competence and then used as a stimulator. Microsomes and plasma membranes were prepared from HSV-Meth A and their immunogenic activities were determined. Through the recovery of stimulatory activity in the plasma membrane fraction was half of that in the microsome fraction, the activity in the former was much more stable than in the latter and the plasma membrane fraction proved to be well qualified as an immunogen for anti-HSV CTL induction. Upon purification, the specific activity of the membrane fraction, on the basis of protein concentration, increased 43-fold.
D000818:Animals; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D006561:Herpes Simplex / Q000276:immunology*; D007111:Immunity, Cellular; D051379:Mice; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*