Caffeine-induced current in embryonic heart cells: time course and voltage dependence.
Abrupt exposure of 90- to 130-micron diameter chick embryonic myocardial cell aggregates to 10 mM caffeine has been shown to induce a transient inward current. In the present study, we recorded a similar current in small cell clusters (less than 10 cells) in which access of caffeine to each of the cells was rapid. The resulting inward current consisted of a single peak, which decayed exponentially (predominant time constant 335 +/- 130 ms at -40 mV) and had a peak amplitude of up to 15.5 microA/cm2. The caffeine-induced current persisted when the slow inward current was abolished by a 30-s pretreatment with 2 microM D 600 and could be observed at potentials where the fast sodium channels were fully inactivated. The current-voltage relation of the caffeine response was linear between -110 and -40 mV, giving an extrapolated voltage intercept of +12 mV. However, the inward current did not diminish or reverse with further depolarization. A substantial inward current occurred at potentials up to +60 mV, which is more positive than the reversal potential of the tetrodotoxin-sensitive inward current. We conclude that the caffeine-induced current is mediated in part by electrogenic Na+-Ca2+ exchange.
D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D004553:Electric Conductivity; D004558:Electric Stimulation; D006321:Heart / Q000187:drug effects / Q000196:embryology / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects
D002110:Caffeine