Novel differences in the characteristics of spleen and peripheral blood lymphocytes activated by anti-immunoglobulin.
A comparison of splenocytes and peripheral blood lymphocytes with regard to activation by anti-immunoglobulin revealed distinctions in the behavior of the populations. Thus, a three- to fourfold greater concentration of anti-immunoglobulin was required to achieve one-half maximal activation of splenocytes than that required for peripheral blood lymphocytes. This difference cannot be accounted for on the basis of binding data. That is, although the binding of 125I-labeled anti-immunoglobulin was found to be slightly greater for peripheral blood lymphocytes than for spleen cells, the concentration required for one-half maximal binding was essentially the same for the two populations. Other differences between the populations were also observed. For example, splenocytes but not peripheral blood lymphocytes were substantially inhibited in terms of activation by anti-immunoglobulin subsequent to the absorption and elution of cells from anti-immunoglobulin-coated petri plates at 4 degrees C, despite the fact that the recovery of cells in general and B cells in particular following absorption and elution from anti-immunoglobulin coated plates at 4 degrees C was essentially the same for both populations.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology*; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology*; D001666:Binding Sites, Antibody; D001773:Blood Cells / Q000276:immunology*; D002448:Cell Adhesion; D002469:Cell Separation; D004306:Dose-Response Relationship, Immunologic; D008213:Lymphocyte Activation; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013154:Spleen / Q000166:cytology
D000888:Antibodies, Anti-Idiotypic; D011947:Receptors, Antigen, B-Cell