Distinguishing characteristics of a new neuroblastoma cell line.
The characteristics of a new neuroblastoma cell line (MC-NB-1) established from the bone marrow of a 2-year-old male are described. Morphologically, the cells appear as flattened and epithelial-like or as small and spherical. Electron microscopy demonstrated microtubules and dense core secretory granules. The doubling time was approximately 35 hr. Isoenzyme patterns and catecholamine secretion indicated a human line of neuronal origin. The soft agar tumor colony forming system demonstrated drug resistance in vitro comparable to in vivo nonresponsiveness. The stemline karyotype of MC-NB-1 is 44,Y,del(1) (p22:), -4, -7, +del(7)(q22:), -16, +t(7;16)(16pter leads to 16q24::7q22 leads to 7q32), -17. Additionally, double-minute bodies were observed. However, no evidence of homogeneous staining regions (HSRs) were detected.
D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002395:Catecholamines / Q000378:metabolism; D002460:Cell Line; D002675:Child, Preschool; D002871:Chromosome Banding; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007621:Karyotyping; D008297:Male; D009447:Neuroblastoma / Q000473:pathology* / Q000503:physiopathology / Q000648:ultrastructure
D002395:Catecholamines; D007527:Isoenzymes; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C020118:ESD protein, human