title: [Use of Wheat Germ Lectin-Sepharose for isolating pregnancy-specific protein].
abstract: Specific pregnancy protein was obtained from retroplacental blood serum by saline fractionation, affinity chromatography on Wheat germ Lectin-Sepharose, gel filtration on Sephadex G-200, hydrophobic chromatography on Phenyl-Sepharose, and ion exchange chromatography on DEAE-Sephacel. The purified preparation of specific pregnancy protein is highly suitable for preparing antisera and the development of immuno- and radioassays of the protein in question.
mesh_terms: D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005260:Female; D006801:Humans; D037102:Lectins; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000302:isolation & purification*; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000302:isolation & purification*; D012685:Sepharose; D014909:Wheat Germ Agglutinins
chemical_list: D037102:Lectins; D011257:Pregnancy Proteins; D011268:Pregnancy-Specific beta 1-Glycoproteins; D014909:Wheat Germ Agglutinins; D012685:Sepharose
vernacular_title: Ispol'zovanie Wheat Germ Lectin-Sepharose dlia vydeleniia spetsificheskogo belka beremennosti.