A mouse monoclonal antibody detecting the allospecificity HLA-A3.
Balb/c mice were immunized with a human B-lymphoblastoid cell line typed HLA-A3, B7. The splenocytes of the immunized mice were fused with a murine myeloma. Supernatants of the cultures were screened against the immunizing cell line in fluorochromasia. Positive cultures were expanded and cloned. One of the clones, X 15.4, was expanded and brought to ascites in Balb/c mice. Monoclonality of the antibody X 15.4, which belongs to the class IgM and immunoprecipitates a molecule of 44000 daltons, was demonstrated by isoelectric focusing. By complement dependent cytotoxicity the ascites only reacted with the lymphocytes of all HLA-A3 individuals from a panel of 146 donors, showing no crossreactions. X 15.4 appears to be one of the very rare xenomonoclonal antibodies suitable for HLA typing.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D001402:B-Lymphocytes; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D005260:Female; D006680:HLA Antigens / Q000032:analysis* / Q000235:genetics; D015791:HLA-A3 Antigen; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007519:Isoantigens / Q000032:analysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010375:Pedigree
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D006680:HLA Antigens; D015791:HLA-A3 Antigen; D007519:Isoantigens