[Improved procedure for isolation and purification of methionine gamma-lyase from Pseudomonas putida].
An improved, simplified and relatively rapid procedure is developed for isolation and purification of a new antitumor enzyme--methionine gamma-lyase from Pseudomonas putida. The method includes five steps instead of seven steps in previous procedure with a good yield of the enzyme. The purified enzyme was shown to be homogeneous by the criteria of disc gel electrophoresis. The highly homogeneous preparations of the enzyme exhibited the absorption maxima at 280 and 420 nm. The detailed studies on antileukemic activity of the methionine gamma-lyase are currently in progress.
D000970:Antineoplastic Agents; D013437:Carbon-Sulfur Lyases / Q000302:isolation & purification* / Q000378:metabolism / Q000627:therapeutic use; D006801:Humans; D007938:Leukemia / Q000188:drug therapy; D008190:Lyases / Q000302:isolation & purification*; D011549:Pseudomonas / Q000201:enzymology*; D013053:Spectrophotometry
D000970:Antineoplastic Agents; D008190:Lyases; D013437:Carbon-Sulfur Lyases; C021558:L-methionine gamma-lyase
Usovershenstvovannyĭ metod vydeleniia i ochistki metionin-gamma-liazy iz Pseudomonas putida.