Effect of Mycoplasma pulmonis infection on protein and glycosaminoglycan synthesis of cultured connective tissue cells.
Human synovial cells, fetal skin fibroblasts and rat granulation tissue fibroblasts were experimentally infected with Mycoplasma pulmonis, a species identified as a contamination of cell cultures, and studied for collagen, total protein and glycosaminoglycan synthesis. Hyaluronic acid and sulfated glycosaminoglycan synthesis were stimulated in cultures where the infection reduced cell density, while they were retarded in cultures which had proliferated into higher density than the controls. An extra polypeptide with molecular weight of 20 kD was seen in [35S]methionine-labelled cells. Media of rat granulation tissue cells showed a shift of a 39-42 kD polypeptide to 33-36 kD position in [35S]methionine and [3H]proline labellings. Other minor changes were also noticed. Collagen synthesis or procollagen conversion to collagen were, however, not altered.
D000818:Animals; D002452:Cell Count; D002462:Cell Membrane / Q000648:ultrastructure; D002478:Cells, Cultured / Q000382:microbiology*; D003094:Collagen / Q000096:biosynthesis; D003238:Connective Tissue / Q000378:metabolism / Q000382:microbiology*; D003239:Connective Tissue Cells; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis; D008715:Methionine / Q000378:metabolism; D009174:Mycoplasma / Q000502:physiology*; D011392:Proline / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats
D006025:Glycosaminoglycans; D006820:Hyaluronic Acid; D003094:Collagen; D011392:Proline; D008715:Methionine