The reactivities of Bauhinia purpurea and Lens culinaris lectins to mouse B Lymphocytes and their subsets.
The use of lectins for the separation of murine spleen lymphocyte subsets was examined. Bauhinia purpurea agglutinin was found to be effective for the enrichment of B cells by differential agglutination. When the differential agglutination technique using lectins was applied to the separation of B cell subsets, Lens culinaris agglutinin was found to be able to fractionate B cells into high responding and low responding subsets for dextran sulfate. A different expression of cell surface IgD was also observed between the cell populations showing different agglutinability with Lens culinaris agglutinin.
D000818:Animals; D001402:B-Lymphocytes / Q000145:classification* / Q000276:immunology; D002469:Cell Separation; D005260:Female; D007700:Kinetics; D037102:Lectins / Q000276:immunology*; D051379:Mice; D008811:Mice, Inbred DBA; D013154:Spleen / Q000166:cytology
D037102:Lectins