Steroid receptors in human endometrium. Comparison of data obtained by three different methods.
To find a method for steroid receptor measurement in small endometrial tissue samples (less than 100 mg), an isoelectric focusing assay has been compared with a dextran-coated charcoal assay for oestradiol receptor. The results correlated well (r = 0.85) and this indicates that isoelectric focusing is a good technique for oestradiol receptor determination. Te isoelectric focusing of progesterone receptor has been compared with a dextran-coated charcoal assay and sucrose density gradient centrifugation. Isoelectric focusing gave recoveries of 0-26% compared to receptor values obtained with the two other methods, which correlated well (r = 0.97). The low recovery implies that the isoelectric focusing assay is not suitable for progesterone receptor determination.
D002499:Centrifugation, Density Gradient / Q000379:methods; D003600:Cytosol / Q000032:analysis; D004717:Endometrium / Q000032:analysis*; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D011959:Receptors, Estradiol; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*
D011959:Receptors, Estradiol; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone