title: Evidence for a negative modulating effect of erucic acid on the peroxisomal beta-oxidation enzyme system and biogenesis in rat liver.
abstract: In male rats fed a 30 cal% high-erucic acid (22:1 (13) (cis] rapeseed oil diet for 4 weeks a transient, small increase in peroxisomal beta-oxidation was observed in liver homogenates and isolated peroxisomes after approximately 1 week. Morphometric analysis revealed a progressive decrease in the average size of the liver peroxisomes (by approx. 20%), as well as their volume fraction (by more than 40%). A negative dose-response was observed when peroxisomal beta-oxidation was determined in animals fed rapeseed oil diets with a variable content of erucic acid. When erucic acid was given as subcutaneous injections the peroxisomal beta-oxidation decreased, in both liver homogenates and isolated peroxisomes. In contrast to recent proposals, our findings indicate that the amount of erucic acid cannot account for the small increase in peroxisomal beta-oxidation observed in the liver of rats adapted to a diet containing 30 cal% rapeseed oil with a high content of this fatty acid. In fact, by the selected criteria erucic acid seems to have a negative modulating effect on both the peroxisomal beta-oxidation enzyme system and biogenesis.
mesh_terms: D015094:3-Hydroxyacyl CoA Dehydrogenases; D000220:Adaptation, Biological; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004746:Enoyl-CoA Hydratase; D004883:Erucic Acids / Q000494:pharmacology*; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D008830:Microbodies / Q000187:drug effects / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors; D009821:Oils; D010084:Oxidation-Reduction / Q000187:drug effects*; D010088:Oxidoreductases / Q000037:antagonists & inhibitors; D010938:Plant Oils; D000074262:Rapeseed Oil; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004883:Erucic Acids; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; D009097:Multienzyme Complexes; D009821:Oils; D010938:Plant Oils; D000074262:Rapeseed Oil; C030756:palmitoyl-CoA dependent dehydrogenase; D010088:Oxidoreductases; D015094:3-Hydroxyacyl CoA Dehydrogenases; D004746:Enoyl-CoA Hydratase