Sympathetic ganglion neurons from aged humans grown in monolayer culture.
Sympathetic ganglia were dissected from aged patients during surgical operation done for a therapeutic purpose in the University Hospital. Nerve cells were isolated from the ganglia by trituration after incubation with collagenase, and were grown on collagen-coated plastic dishes with a growth medium. These nerve cells regenerated their axons and survived in the tissue culture for more than 3 weeks. The nerve cells tended to reduce the size in an early stage of the in vitro growth.
D000375:Aging; D001369:Axons / Q000648:ultrastructure; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D005728:Ganglia, Sympathetic / Q000166:cytology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008722:Methods; D008875:Middle Aged; D009474:Neurons / Q000166:cytology* / Q000648:ultrastructure
D003470:Culture Media