In vivo blockage of B cell maturation by splenic T cells of total lymphoid irradiated mice.
The effect of fractionated total lymphoid irradiation (TLI) on the induction of cells blocking in vivo B cell maturation was examined. (BALB/c x C57BL/6)F1 mice received 200 rds lymphoid irradiation daily for 8 days. One month after termination of the irradiation splenic T cells of TLI-treated mice were transferred, together with normal bone marrow cells, to lethally irradiated hosts. In addition, splenic cells or splenic B cells of TLI mice were transferred to irradiated hosts. Two months later, we checked the reconstituted mice for their ability to produce in vivo anti-trinitrophenyl (TNP)-Ficoll antibodies and their capacity to respond in vitro to dextran sulfate and lipopolysaccharide. In parallel, stained spleen cells were analyzed on the fluorescence-activated cells sorter using monoclonal anti-mu, anti-delta and anti-B220 surface marker antibodies. The results indicate that splenic T cells originating from TLI-treated mice cause maturation arrest of normal and TLI-treated B cells, since normal bone marrow cells injected together with those cells have immature phenotypic and functional features. In addition, neither splenic cells nor splenic B cells of TLI-treated mice can mature upon transfer to normal irradiated hosts. The B cells are IgM+, IgD-; they respond with a high proliferation rate to dextran sulfate and low proliferation rate to lipopolysaccharide, and poorly to TNP-Ficoll challenge.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000528:radiation effects*; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D007072:Immunoglobulin D / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010641:Phenotype; D013154:Spleen / Q000276:immunology / Q000528:radiation effects; D013601:T-Lymphocytes / Q000276:immunology / Q000528:radiation effects*
D007072:Immunoglobulin D; D007075:Immunoglobulin M