Effect of pyridoxine on the inhibition by dexamethasone of murine erythroleukemia cell differentiation.
Glucocorticoids are known to inhibit the induced differentiation of murine erythroleukemia cells. I show here that the binding of glucocorticoid receptors to the intact nuclei of these cells is reduced by exogenous extracellular pyridoxine (1-4 mM). This reduction of glucocorticoid-receptor binding did not abrogate the inhibition by dexamethasone of the induced differentiation.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003907:Dexamethasone / Q000378:metabolism / Q000633:toxicity*; D006454:Hemoglobins / Q000032:analysis; D007700:Kinetics; D007942:Leukemia, Experimental / Q000503:physiopathology*; D051379:Mice; D011736:Pyridoxine / Q000494:pharmacology*; D011965:Receptors, Glucocorticoid / Q000187:drug effects / Q000502:physiology*; D011987:Receptors, Steroid / Q000502:physiology*
D006454:Hemoglobins; D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D003907:Dexamethasone; D011736:Pyridoxine