[Unfolding of nucleosome cores induced by chemical acetylation of histones].
Relative accessibility of nucleosomal histones to acetic anhydride during acetylation has been studied as a function of concentration, pH and ionic strength of the solution using high-resolution gel-electrophoresis. It was shown that about 80% of lysine residues in nucleosomal histones and 100% of the same residues in histone complexes without DNA in 2 M NaCl are accessible to the modification, which is proved by the localization of the majority of lysine residues in nucleosomes near the surface of the histone octamer, by their participation in ionic interactions with DNA and, probably, in histone-histone contacts. Gel-electrophoretic experiments with nucleosomes and studies of the histone resistance to mild trypsinolysis indicated that neither nucleosomes themselves nor histone octamers are affected even though 50% of lysine residues in histones have been acetylated. The process of acetylation is accompanied by the growing tendency of histones to participate in mild trypsinolysis and by a gradual decline in electrophoretic mobility and in the value of the sedimentation constant. The circular dichroism spectra and the microscopic appearance of nucleosomes are also markedly changed. These results suggest that a gradual unfolding of nucleosomes occurs when 5 or more lysine residues in the nucleosomal histones have been acetylated.
D000085:Acetates / Q000494:pharmacology*; D000089:Acetic Anhydrides / Q000494:pharmacology*; D000107:Acetylation; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D004247:DNA / Q000378:metabolism; D006657:Histones / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D051379:Mice; D008970:Molecular Weight; D009707:Nucleosomes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D009994:Osmolar Concentration
D000085:Acetates; D000089:Acetic Anhydrides; D006657:Histones; D009707:Nucleosomes; C031800:acetic anhydride; D004247:DNA
Razvorachivanie korovykh nukleosom, vyzvannoe khimicheskim atsetilirovaniem gistonov.