title: Changes in [Ca2+]i induced by rapid cooling of single skeletal muscle fibres treated with low concentration of caffeine.
abstract: In single skeletal muscle fibres treated with low concentration of caffeine, lowering the bathing solution temperature from 18 degrees C to below 7 degrees C increased [Ca2+]i in three phases depending on caffeine concentration and temperature. Tension could be fully developed (rapid cooling contracture, RCC) by the second phase of the released Ca2+. The second and third phases were inhibited by low concentrations of procaine, and the first phase was blocked by a higher one. RCC was observed even in Ca2+-free solution. The mechanism of [Ca2+]i changes and RCC during cooling was discussed.
mesh_terms: D000331:Aequorin; D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D003080:Cold Temperature; D066298:In Vitro Techniques; D009132:Muscles / Q000187:drug effects / Q000502:physiology*; D011896:Rana temporaria
chemical_list: D002110:Caffeine; D000331:Aequorin; D002118:Calcium