Resorption of implanted bone prepared from normal and warfarin-treated rats.
Bone that was virtually depleted of the vitamin K-dependent protein, osteocalcin, and 93% reduced in the concentration of its characteristic amino acid, gamma-carboxyglutamic acid, was obtained from rats treated with warfarin for 6 wk. Osteocalcin-deficient bone particles were resistant to resorption when implanted subcutaneously in normal rats. The relative resorption was 60% of control bone, as measured by histomorphometry as percent of bone particles in the field. Additionally, the number of multinucleated cells around the bone particles was reduced by 54%. These data suggest that osteocalcin is an essential component for bone matrix to elicit progenitor-cell recruitment and differentiation necessary for bone resorption.
D000818:Animals; D001857:Bone Matrix / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D001862:Bone Resorption / Q000187:drug effects*; D002135:Calcium-Binding Proteins / Q000172:deficiency / Q000502:physiology; D002454:Cell Differentiation; D008297:Male; D015675:Osteocalcin; D051381:Rats; D013234:Stem Cells / Q000166:cytology; D014859:Warfarin / Q000494:pharmacology*
D002135:Calcium-Binding Proteins; D015675:Osteocalcin; D014859:Warfarin