[Purification and isolation of the arom aggregates of Schizosaccharomyces pombe].
The five enzymes that catalyzing steps two through six in the prechorismate polyaromatic amino acid biosynthetic pathway are physically associated and have been purified up to 400-fold from Schizosaccharomyces pombe. The native arom aggregate has a molecular weight of approx. 140,000-145,000 based on gel filtration, glycerol-density-gradient centrifugation, and polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate. Similarities between the S. pombe arom aggregate and that of Neurospora crassa and Euglena gracilis are discussed.
D000429:Alcohol Oxidoreductases; D000596:Amino Acids / Q000032:analysis; D001203:Ascomycota / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000302:isolation & purification; D006836:Hydro-Lyases; D008190:Lyases; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000302:isolation & purification*; D017853:Phosphotransferases (Alcohol Group Acceptor); D012568:Schizosaccharomyces / Q000201:enzymology*; D014166:Transferases
D000596:Amino Acids; D005656:Fungal Proteins; D009097:Multienzyme Complexes; C019728:arom enzyme; D000429:Alcohol Oxidoreductases; D014166:Transferases; D017853:Phosphotransferases (Alcohol Group Acceptor); D008190:Lyases; D006836:Hydro-Lyases
Reinigung und Gewinnung des arom-Aggregates von Schizosaccharomyces pombe.