The effect of phospholipid bilayers on AMP-deaminase from rat tissues.
The ATP-activated adenylate deaminase (EC 3.5.4.6) from the rat heart, kidney, spleen, liver and brain showed a mixed-type allosteric activation under the influence of phosphatidylcholine-containing liposomes, but no effect was observed on the non-activated enzyme isolated from all these tissues but the brain. The brain enzyme was activated by the liposomes also in the absence of ATP. No effect of the phosphatidylcholine-containing liposomes could be shown on the adenylate deaminase isolated from skeletal muscle and red blood cells. The phosphatidate-containing liposomes exerted an inhibitory effect on the enzyme isolated from the heart, kidney and brain, being without effect on the enzyme from skeletal muscle.
D000659:AMP Deaminase / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D008081:Liposomes / Q000494:pharmacology*; D009709:Nucleotide Deaminases / Q000378:metabolism*; D009928:Organ Specificity; D010712:Phosphatidic Acids / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000494:pharmacology; D051381:Rats; D014018:Tissue Distribution
D008081:Liposomes; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D000255:Adenosine Triphosphate; D009709:Nucleotide Deaminases; D000659:AMP Deaminase