[Comparative analysis of the interaction of highly purified glucocorticoid-receptor complexes from rat liver with homologous and heterologous cell nuclei].
The binding of glucocorticoid-receptor complexes (GRC) from rat liver purified 1000-2000-fold to homologous and heterologous cell nuclei was investigated. The purified GRC preparations were examined by gel filtration, ion-exchange chromatography on DEAE-cellulose and electrophoresis in polyacrylamide gel in the presence of Na-DS. The binding of GRC to the nuclei was characterized in terms of associate resistance to KCl. It was found that the isolated nuclei of the non-homologous tissue of the same species (rat brain) or of the heterologous tissues (liver and brain) of phylogenetically different species (pigeon, frog) can bind rat liver GRC in the same degree or even more than the homologous nuclei. Some tissue- and species-dependent peculiarities of GRC binding capacity of the nuclei were revealed. The GRC binding capacity of pigeon nuclei is somewhat higher than that of rat nuclei, while the corresponding ability of frog brain nuclei is higher than that of frog liver nuclei. The GRC-nuclei associates are more resistant to KCl than the GRC--pure DNA associates.
D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000302:isolation & purification / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid
Sravnitel'nyĭ analiz vzaimodeĭstviia vysokoochishchennykh gliukokortikoid-retseptornykh kompleksov pecheni krys s gomologichnymi i geterologichnymi kletochnymi iadrami.