Sensitivity of commercial agglutination and counterimmunoelectrophoresis methods for the detection of Haemophilus influenzae Type b capsular polysaccharide.
Comparison testing of commercial latex agglutination, coagglutination, and counterimmunoelectrophoresis (CIE) reagents for the detection of Haemophilus influenzae Type b capsular antigen in cerebral spinal fluid was performed. Latex agglutination was the most sensitive (0.2 ng/mL), followed by coagglutination (10 ng/mL), and CIE (20 ng/mL). In addition, the commercial antisera for CIE failed to react with high concentrations of capsular antigen, well within the range found during the course of meningitis.
D000372:Agglutination Tests / Q000379:methods; D003378:Counterimmunoelectrophoresis; D005069:Evaluation Studies as Topic; D006193:Haemophilus influenzae / Q000276:immunology*; D006801:Humans; D007841:Latex Fixation Tests; D011134:Polysaccharides / Q000032:analysis* / Q000134:cerebrospinal fluid; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000134:cerebrospinal fluid
D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; C008984:polyribitol phosphate