24R,25-dihydroxyvitamin D stimulates creatine kinase BB activity in chick cartilage cells in culture.
In chick limb-bud cartilage cell cultures 24R,25-dihydroxycholecalciferol (24R,25(OH)2D3), but not 24S,25(OH)2D3, 1 alpha,25(OH)2D3 or 25(OH)D3, stimulates the activity of the brain type (BB) isozyme of creatine kinase (EC 2.7.3.2), the 'estrogen-induced protein' first identified in rat uterus. Cultures treated with bromodeoxyuridine, in which cartilage formation is inhibited, show no stimulation of creatine kinase BB by 24R,25(OH)2D3.
D015650:24,25-Dihydroxyvitamin D 3; D000818:Animals; D002356:Cartilage / Q000201:enzymology*; D002478:Cells, Cultured; D002645:Chickens; D003402:Creatine Kinase / Q000378:metabolism*; D004100:Dihydroxycholecalciferols / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D007527:Isoenzymes
D004100:Dihydroxycholecalciferols; D007527:Isoenzymes; D015650:24,25-Dihydroxyvitamin D 3; D003402:Creatine Kinase