[A rapid method for assay of 25-hydroxyvitamin D in serum].
A simplified assay for 25-hydroxyvitamin D is reported. In this procedure ethanol extraction is used in order to obtain a better protein precipitation and a higher recovery of added tritiated 25-hydroxyvitamin D3. Extraction is followed by column chromatography on Sep-pak Silica cartridges. The eluate is dried and directly used for competitive protein binding assay. The method shows two main advantages: it is less time consuming and is easier to perform than existing procedures.
D015652:25-Hydroxyvitamin D 2; D004872:Ergocalciferols / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D008722:Methods
D004872:Ergocalciferols; D015652:25-Hydroxyvitamin D 2
Metodo rapido per il dosaggio della 25-idrossivitamina D nel siero.