[Detection of hybrid cells secreting monoclonal immunoglobulins using Staphylococcus aureus agglutination].
A quantitative assay for mouse monoclonal immunoglobulins (Ig) was made possible by a very simple and sensitive co-agglutination test. Staphylococci which were rich in protein A were used in the presence of sheep anti-mouse IgG (H + L) antibodies. The assay was conducted in a single step using micro-haemagglutination plates. Plates were scored after over-night incubation. This method could detect as little as 0.006 microgram of IgG/ml. One set of hybrids was screened both by this method and by an immunoenzymatic assay (ELISA): with one exception, all clones which were found to produce Ig by ELISA were also positive by co-agglutination. Moreover, the co-agglutination method detected 7 additional Ig-secreting cultures. The level of secretion of each positive hybridoma ranged from 0.2 to 2 micrograms/ml. Thus, when compared to ELISA, the co-agglutination test was not only just as sensitive, but was also much simpler and faster.
D000372:Agglutination Tests / Q000379:methods; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D000921:Antibody-Producing Cells / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012756:Sheep; D013205:Staphylococcal Protein A / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A
Détection des hybrides sécréteurs d'immunoglobulines monoclonales par agglutination de Staphylococcus aureus.