[Surface glycoproteins of normal and malignant leukocytes].
This study aimed to evaluate the molecular weight distribution patterns and the quantitative expression of surface glycoproteins (S-GP) of various differentiation stages of human leucocytes. S-GP were first exposed by treatment with neuraminidase; subsequently they were labelled by galactose-oxidase treatment followed by reduction with 3H-sodium borohydride. Labelled S-GP were separated on polyacrylamide gels in the presence of SDS and were visualized by means of fluorography. A total of 8 major S-GP bands with apparent molecular weights of 230 000, 215 000, 200 000, 185 000, 175 000, 150 000, 125 000 and 110 000 daltons were identified. All of these S-GP were already expressed at the level of pluripotent myelopoietic stem cells but different in their relative expression during further cellular maturation.
D001402:B-Lymphocytes / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006098:Granulocytes / Q000378:metabolism; D006689:Hodgkin Disease / Q000378:metabolism*; D006801:Humans; D007938:Leukemia / Q000378:metabolism*; D007962:Leukocytes / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism
D006023:Glycoproteins; D008565:Membrane Proteins
Oberflächenglykoproteine an normalen und malignen Leukozyten.