Isolation and properties of alpha-amylase from perienteric fluid of Ascaris suum.
The properties of alpha-amylase from perienteric fluid of Ascaris suum were studied. The enzyme is strongly activated by chlorides at a simultaneous shift of pH optimum. It is fully activated already at 10(-3) M concentration of chlorides. A method of alpha-amylase isolation was modified with respect to these properties to give the purification degree 278 and yield of 11%.
D000327:Adsorption; D000818:Animals; D001200:Ascaris / Q000201:enzymology*; D002712:Chlorides / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D013213:Starch / Q000378:metabolism; D013552:Swine / Q000469:parasitology; D013696:Temperature; D000516:alpha-Amylases / Q000302:isolation & purification* / Q000378:metabolism
D002712:Chlorides; D013213:Starch; D000516:alpha-Amylases