title: Ribosomal subunits and ribosomal proteins of Tetrahymena thermophila. Effect of the presence of iodoacetamide during ribosome extraction on the properties of the subunits.
abstract: Proteolytic degradation of ribosomal proteins occurs during the preparation of subunits of the cytoplasmic ribosomes of the protozoa Tetrahymena thermophila and the isolated subunits are inactive. Addition of 5 mM iodoacetamide to cell suspensions before extraction inhibits proteolytic activity and permits isolation of active subunits. The protein complements of these subunits have been characterized in two different two-dimensional electrophoretic systems, and their molecular weights have been determined.
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D007460:Iodoacetamide / Q000494:pharmacology*; D007461:Iodoacetates / Q000494:pharmacology*; D008970:Molecular Weight; D012269:Ribosomal Proteins / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D012270:Ribosomes / Q000032:analysis*; D013768:Tetrahymena / Q000032:analysis*
chemical_list: D007461:Iodoacetates; D012269:Ribosomal Proteins; D007460:Iodoacetamide