An improved microindentation technique to measure changes in properties of arterial intima during atherogenesis.
A fully automated instrument was developed that measured the amount and time-course of indentation of arterial intima by a spherical tipped probe 25 micrometers in diameter loaded routinely by forces of 10 microN. The relationship of depth of indentation to time was non-exponential but reached an apparent asymptotic value in about 6 s. Routine indentations were recorded 10 s after loading, the relationship of indentation and force being essentially linear between 0-100 microN. The instrument is an order of magnitude more sensitive than one previously described (Gow and Vaishnav, 1975, J. Appl. Physiol. 38, 344-350) and produces indentations of 1-4 micrometers in the intima of rabbit thoracic aorta with a reproducibility of 1-2%. Preliminary usage of the new microindentor has shown increases in the compliance of the thoracic aorta intima in rabbits during cholesterol feeding.
D000818:Animals; D001013:Aorta, Thoracic / Q000503:physiopathology; D001158:Arteries / Q000503:physiopathology*; D001161:Arteriosclerosis / Q000209:etiology / Q000503:physiopathology*; D001696:Biomechanical Phenomena; D006439:Hemodynamics; D066298:In Vitro Techniques; D011817:Rabbits; D013314:Stress, Mechanical; D013696:Temperature