title: Cultured human T lymphocyte lines and clones as immunogenetic tools.
abstract: Human T lymphocytes were sensitized in vitro to allogeneic determinants encoded by the HLA region and subsequently cloned by limiting dilution in the presence of human T cell growth factor. An in vitro priming protocol entailing multiple rounds of allostimulation in mixed lymphocyte culture, using a disparate stimulating to responding cell ratio (10:1), enabled a high frequency of functionally active clones to be isolated. The specificity of the proliferative and cytotoxic reactivities was ascertained directly by screening in an HLA characterized family.
mesh_terms: D000483:Alleles; D002478:Cells, Cultured; D002999:Clone Cells; D046508:Culture Techniques / Q000379:methods; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology; D008297:Male; D013601:T-Lymphocytes / Q000276:immunology* / Q000528:radiation effects
chemical_list: D007376:Interleukin-2