Role of monocytes in the augmentation of human natural killer cell activity by interleukin-2.
The cellular requirement(s) and mechanism(s) involved in the augmentation of human natural killer cell (NK) activity by Interleukin (IL-2) were studied. NK activity was enhanced by IL-2 in the absence of monocytes. This potentiation of IL-2 on NK enhancement was suppressed by PGE2, and was enhanced by indomethacin in the experiments using unfractionated mononuclear cells. When monocyte-depleted cells were stimulated with IL-2, the cytotoxic activity was greater than that seen with unfractionated mononuclear cells. Monocytes in culture produced PGE2 which partially reduced NK enhancement by IL-2. These findings suggest that PGE2, produced by monocytes, regulates the NK enhancement by IL-2 in vitro.
D000328:Adult; D015232:Dinoprostone; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007376:Interleukin-2 / Q000502:physiology*; D007694:Killer Cells, Natural / Q000187:drug effects / Q000502:physiology*; D009000:Monocytes / Q000502:physiology*; D011070:Poly I-C / Q000494:pharmacology; D011458:Prostaglandins E / Q000096:biosynthesis / Q000494:pharmacology
D007376:Interleukin-2; D011458:Prostaglandins E; D015232:Dinoprostone; D011070:Poly I-C; D007213:Indomethacin