Mouse rosette positive B cells fail to synthesize immunoglobulin following incubation with pokeweed mitogen.
Mouse erythrocytes form spontaneous rosettes with a population of B lymphocytes from normal individuals and from patients with B cell chronic lymphocytic leukaemia (CLL). Since lymphocytes from patients with CLL respond poorly to pokeweed mitogen (PWM), we have compared mouse rosette positive (MR+), mouse rosette negative (MR-), and unfractionated B lymphocytes from normal individuals in their response to PWM. Mononuclear cells were fractionated into B, MR+ and MR- cell populations and then combined in 1:1 proportions with mitomycin-C treated T cells in culture media. Lymphocyte co-cultures were incubated for up to 10 days in the presence of PWM. Supernatant immunoglobulin (SIg) levels, percentage intracytoplasmic immunoglobulin (ICIg), and proliferative responses were determined. MR+ cells alone failed to produce significant levels of SIg (P less than 0.025) or percentages of ICIg positive cells This decreased synthesis of immunoglobulin by MR+ cells occurred in the presence of adequate T cells, macrophages and a satisfactory proliferative response.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000145:classification; D008264:Macrophages / Q000276:immunology; D051379:Mice; D011043:Pokeweed Mitogens / Q000494:pharmacology*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology
D007136:Immunoglobulins; D011043:Pokeweed Mitogens