Modulation of the metastatic capability in B16 melanoma by cell shape.
The lung colonization of B16-F1 cells grown in flat and spherical configurations was studied. Cells cultivated in vitro as spheroids on a nonadhesive substrate expressed in a reversible fashion a marked increase in their propensity to establish metastases. The altered metastatic capability was accompanied by a reversible reduction in the accessibility of cell surface proteins to external iodination and by a dramatic decrease in the synthesis of vimentin.
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D007381:Intermediate Filament Proteins / Q000096:biosynthesis; D008175:Lung Neoplasms / Q000556:secondary; D008545:Melanoma / Q000473:pathology*; D008565:Membrane Proteins / Q000502:physiology; D051379:Mice; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000502:physiology; D009374:Neoplasms, Experimental / Q000473:pathology; D014746:Vimentin
D007381:Intermediate Filament Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D014746:Vimentin