title: Immunoregulation by macrophages II. Separation of mouse peritoneal macrophages having tumoricidal and bactericidal activities and those secreting PGE and interleukin I.
abstract: Macrophage subpopulations having bactericidal or tumoricidal activities and secreting interleukin I (IL1) or prostaglandin E (PGE) were identified through primary or secondary infection with Salmonella enteritidis and separated by sedimentation velocity. Bactericidal activity was measured by [3H]-thymidine release from Listeria monocytogenes and tumoricidal activity by 51Cr-release from C-4 fibrosarcoma or P815 mastocytoma cells. Macrophages with bactericidal activity were distinguished from those with tumoricidal activity a) during secondary infection when cytolytic activity occurred only at days 1-4 post injection and bactericidal activity remained high throughout and b) after sedimentation velocity separation. Cytolysis was consistently greatest among adherent cells of low sedimentation velocity, whereas cells with bactericidal activity increased in size during the infection. Tumour cytostasis (inhibition and promotion of [3H]-thymidine uptake) differed from cytolysis in that the former was more prolonged during infection and was also detected among large cells. Secretion of immunoregulatory molecules PGE and IL1 occurred maximally among different macrophage subpopulations separated by sedimentation velocity and depending on the type of stimulus used in vitro. There was an inverse correlation between IL1 production and PGE production after stimulation with C3-zymosan or lipopolysaccharide (LPS). The development of immunity during infection may therefore be dependent upon the relative proportions of effector and regulatory macrophage subpopulations and the selective effects of environmental stimuli on these functions.
mesh_terms: D000818:Animals; D001770:Blood Bactericidal Activity; D002448:Cell Adhesion; D002469:Cell Separation; D002860:Chromium Radioisotopes; D003602:Cytotoxicity, Immunologic; D005354:Fibrosarcoma / Q000276:immunology; D007375:Interleukin-1 / Q000378:metabolism*; D008262:Macrophage Activation; D008264:Macrophages / Q000145:classification / Q000276:immunology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009374:Neoplasms, Experimental / Q000276:immunology; D010587:Phagocytosis; D011458:Prostaglandins E / Q000378:metabolism*; D012480:Salmonella Infections / Q000276:immunology
chemical_list: D002860:Chromium Radioisotopes; D007375:Interleukin-1; D011458:Prostaglandins E