Growth of AKR T cell leukemia lymphoblasts in medium containing interleukin 2 (IL-2).
Long-term cloned mouse leukemic T cell lines were established in vitro using interleukin-2 (IL-2) conditioned media. The cell lines were tested for retention of both antigenic expression and tumorigenicity, as well as for growth characteristics. Several important findings resulted from these studies. A reliable method was developed for consistent success in the culturing and cloning of leukemic T cell lines. Cultured cells from IL-2-dependent lines were found to retain their original histocompatibility and differentiation antigen characteristics for up to 2 yr. Mortality patterns, comparing long-term cloned leukemic T cell lines with fresh AKR leukemia cells, showed that the cloned cells had greater tumorigenicity. An especially interesting finding was that cell lines established both from different mice and from a single organ of an individual mouse were heterogeneous with respect to antigenic makeup, cell growth kinetics, and tumorigenicity. Finally, because of their dependence on IL-2, the cloned tumor cell lines provided excellent index cells to quantify IL-2 activity.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D002455:Cell Division; D002460:Cell Line; D002999:Clone Cells / Q000166:cytology; D003470:Culture Media; D006649:Histocompatibility Antigens / Q000276:immunology; D007376:Interleukin-2 / Q000502:physiology*; D007700:Kinetics; D007945:Leukemia, Lymphoid / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008806:Mice, Inbred AKR; D013601:T-Lymphocytes / Q000276:immunology
D000954:Antigens, Surface; D003470:Culture Media; D006649:Histocompatibility Antigens; D007376:Interleukin-2