Schistosoma mansoni: complement activation in human and rodent sera by living parasites of various developmental stages.
Living Schistosoma mansoni of various developmental stages were studied with respect to their ability to activate the complement system in sera of humans, mice and rats. Immunofluorescence assays demonstrated that binding of human C3 occurred on fresh schistosomula as well as on schistosomula prepared from mouse lymph-nodes or lungs and on adult schistosomes. However, rodent C3 was deposited only on fresh schistosomula. Deposition of human C3 on the worms' surface required activation of the complement system. The alternative pathway was shown to be involved in deposition of human C3 on schistosomes of all ages, whereas activation of the classical pathway was demonstrable only with fresh schistosomula. Immunoelectrophoretic studies demonstrated a dose-dependent cleavage of human C3 and conversion of factor B by living adult schistosomes. The results demonstrate that the ability of living schistosomes to activate complement in vitro is dependent not only on their development stage but also on the species of the serum.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D003166:Complement Activating Enzymes / Q000378:metabolism; D003167:Complement Activation; D015922:Complement C1q; D003176:Complement C3 / Q000378:metabolism; D003170:Complement Pathway, Alternative; D003171:Complement Pathway, Classical; D006801:Humans; D008297:Male; D051379:Mice; D011414:Properdin / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012550:Schistosoma mansoni / Q000254:growth & development / Q000276:immunology*; D013045:Species Specificity
D000906:Antibodies; D003176:Complement C3; D011414:Properdin; D015922:Complement C1q; D003166:Complement Activating Enzymes