Proliferative human T cell responses to Chlamydia trachomatis in vitro.
The T cell proliferative response to Chlamydia trachomatis was studied in otherwise healthy persons. A suspension of partially purified C. trachomatis subtype LGV-2 particles was used throughout the study. Studies of cord blood lymphocytes demonstrated that the preparation was not mitogenic. The proliferative capacity of peripheral blood mononuclear cells (PBM) and T + non-T cells from adults was tested; in about 70% a proliferative response was observed. The proliferative responses were dependent upon antigen presenting cells (APC) and were mainly mediated by T cells, even though B cells proliferated to a lesser extent. Using antigen-pulsed non-T cells as APC, a significant and consistent specific proliferative response could be obtained. High responders could be separated from low responders with different T cell concentrations. We also found that the T cell response was restricted by the HLA-D/DR determinants of the T cell donor.
D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002692:Chlamydia trachomatis / Q000276:immunology*; D005312:Fetal Blood / Q000276:immunology; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000276:immunology*
D000942:Antigens, Bacterial; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II