Stimulation of secondary anti-MSV cytolytic T lymphocytes with MBL-2 reconstituted membranes.
Membranes and solubilized, reconstituted membranes from Moloney-infected tumors MBL-2 have been used to stimulate in vitro secondary cytolytic T lymphocytes (CTL) in C57BL/6 mice primed and Moloney murine sarcoma virus. Membranes are shown to stimulate the generation of Moloney-specific and H-2 restricted CTL. Stimulation with solubilized, reconstituted membranes required the presence of rat lymphocyte Con A supernatant (containing interleukin 2) during the culture. Reconstituted membranes made in the presence of the detergent-insoluble fraction from the plasma membrane were able to stimulate a response in the absence of Con A supernatant.
D000818:Animals; D002462:Cell Membrane / Q000276:immunology; D002472:Cell Transformation, Viral; D005260:Female; D007156:Immunologic Memory; D007376:Interleukin-2 / Q000276:immunology; D051379:Mice; D008979:Moloney murine leukemia virus; D012513:Sarcoma, Experimental / Q000276:immunology*; D012995:Solubility; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D007376:Interleukin-2