title: Surface immunoglobulin phenotype of complement receptor-positive and complement receptor-negative B cells of normal and xid immunodeficient mice.
abstract: Single- and dual-parameter fluorescence-activated cell sorter analyses of complement receptor-positive and -negative (CR+ and CR-) splenic B cells of normal and xid immunodeficient mice were performed to determine the frequencies of surface (s) IgM+ and sIgD+ cells, as well as the quantities of sIgM and sIgD on these cells. Single parameter analysis revealed identical frequencies of sIgM+ and sIgD+ cells in the CR+ B cell population of normal mice. In contrast, the percentage of sIgM+ cells exceeded that of sIgD+ cells in the CR- normal and CR+ xid B cell population to a similar extent. CR- xid B cells showed an even greater discrepancy between sIgM+ and sIgD+ cells. Dual-parameter analysis of CR+ normal B cells showed the typical sIg phenotype of mature B cells, i.e. an intermediate density of sIgM and a high density of sIgD. The CR+ xid B cell population displayed an increase in cells with high sIgM and no-to-low sIgD and a decrease in cells with intermediate sIgM and high sIgD when compared to CR+ normal cells. CR- B cells from normal and xid immunodeficient mice were similar in that both consisted largely of a cell population with low sIgM and no-to-low sIgD. However, the CR- normal B cell population also contained cells with intermediate sIgM and high sIgD, which were lacking in the CR- xid cell population. Similar results were obtained when CR+ and CR- B cells from normal and xid mice were purified from a small resting B cell population, isolated by elutriation. This indicates that the sIg phenotype of CR- cells is not due to the presence of large cells which have lost sIgD during activation.
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D007072:Immunoglobulin D / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007153:Immunologic Deficiency Syndromes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D011947:Receptors, Antigen, B-Cell / Q000235:genetics*; D011951:Receptors, Complement / Q000276:immunology*; D013154:Spleen / Q000166:cytology
chemical_list: D007072:Immunoglobulin D; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell; D011951:Receptors, Complement