title: Intrinsic factor in human amniotic fluid as determined by radioimmunoassay.
abstract: The intrinsic factor (IF) concentration in 55 human amniotic fluid specimens was determined by radioimmunoassay (RIA). The antiserum was produced by immunizing rabbits with the cobalamin-IF complex isolated from human gastric juice. The median concentration of IF was 0.17 nmol/l and the extreme values less than 0.07-2.51 nmol/l. Three specimens with a clearly elevated level (0.96, 1.11 and 2.51 nmol/l) were observed. The highest value was associated with a fetal malformation, viz. obstruction of the proximal gut. There was no evident correlation between the concentration of IF in amniotic fluid and gestational age.
mesh_terms: D000328:Adult; D000653:Amniotic Fluid / Q000032:analysis*; D002850:Chromatography, Gel; D000013:Congenital Abnormalities / Q000378:metabolism; D005260:Female; D005315:Fetal Diseases / Q000378:metabolism; D006801:Humans; D007437:Intrinsic Factor / Q000032:analysis*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000378:metabolism; D011863:Radioimmunoassay
chemical_list: D007437:Intrinsic Factor