Lipid metabolism in microsomal fraction from photosynthetic tissue. Effects of catalase and hydrogen peroxide on oleate desaturation.
On incubation of microsomal fraction from pea (Pisum sativum L.) leaves with ammonium [1-14C]oleate or [1-14C]oleoyl-CoA in the presence of ATP, CoA, Mg2+ and NADH, the major reactions observed were those catalysed by oleoyl-CoA synthetase, oleoyl-CoA thioesterase, oleoyl-CoA:phosphatidylcholine acyltransferase and oleoyl phosphatidylcholine desaturase. The reaction catalysed by oleoyl phosphatidylcholine desaturase was specifically inhibited by H2O2, and this inhibitory effect was overcome by catalase (EC 1.11.1.6).
D000214:Acyl Coenzyme A / Q000378:metabolism; D002374:Catalase / Q000494:pharmacology*; D007887:Fabaceae / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism*; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism*; D010944:Plants / Q000187:drug effects / Q000378:metabolism*; D010946:Plants, Medicinal
D000214:Acyl Coenzyme A; D009829:Oleic Acids; C017585:oleoyl-coenzyme A; D019301:Oleic Acid; D006861:Hydrogen Peroxide; D002374:Catalase