Effects of puromycin and cycloheximide on properties of cells from Xenopus laevis early embryos.
The effects have been studied of puromycin and cycloheximide on the reaggregation of ectoderm cells dissociated from Xenopus laevis blastulae. Puromycin or cycloheximide can inhibit reaggregation, suggesting that cell reassociation is dependent upon protein synthesis. If the cells are allowed a 3 h 'recovery' period in culture medium following dissociation, before being exposed to either puromycin or cycloheximide, higher concentrations of the inhibitors are required to prevent cell aggregation, suggesting that significant synthesis of the proteins required for reaggregation occurs in the 3 h immediately following dissociation. Lower concentrations of puromycin permit cell reaggregation but reduce the normal formation of cilia. The effects have also been observed of puromycin on the scanning electron microscopical appearance of Xenopus blastula ectoderm cells cultured singly in vitro. Puromycin reduces the normal formation of pseudopodia, suggesting that puromycin might inhibit reaggregation partly by inhibiting cell movement. Puromycin also produces some elongated cells, possibly by inhibition of cytokinesis.
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002449:Cell Aggregation / Q000187:drug effects*; D003513:Cycloheximide / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D011691:Puromycin / Q000494:pharmacology*; D014982:Xenopus laevis / Q000196:embryology*
D011691:Puromycin; D003513:Cycloheximide