A simple and rapid dot-immunobinding assay for zein and other prolamins.
A simple method for the immunochemical assay of zein and other prolamins is described. Alcohol-solubilized zein is spotted onto chromatography paper disks or strips, incubated with antiserum (30-60 min), washed with several changes of Tris-buffered saline (10 min), incubated with protein A-peroxidase (30 min), and washed with several changes of Tris-buffered saline (10 min). the binding of zein-specific antibody is visualized by peroxidase-catalyzed color production from 4-chloro-1-naphthol. The method is simple, inexpensive, and rapid (2 h total time) and has potential application to other insoluble antigens.
D003720:Densitometry; D007118:Immunoassay; D009284:Naphthols; D010544:Peroxidases; D010940:Plant Proteins / Q000032:analysis; D055315:Prolamins; D015028:Zein / Q000032:analysis* / Q000276:immunology
D009284:Naphthols; D010940:Plant Proteins; D055315:Prolamins; C025917:4-chloro-1-naphthol; D015028:Zein; D010544:Peroxidases