Application of high-performance liquid chromatography with electrochemical detection to the determination of catecholamines in microdissected regions of the rat brain.
High-pressure liquid chromatography (HPLC) with electrochemical detection was applied for measurement of catecholamines in microdissected tissues from discrete brain regions. Brain areas were dissected, extracted and processed for HPLC assay without further purification. The advantage of this method is its simplicity and relatively low cost and may therefore be applied to the measurement of amines in brain micropunches.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002395:Catecholamines / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D008297:Male; D009115:Muridae
D002395:Catecholamines