Synthesis of phage-specific transfer RNA molecules by vibriophage phi 149.
32P-Labelled tRNA was isolated from uninfected and phage phi 149-infected Vibrio cholerae cells. These tRNA preparations were then hybridised with DNA isolated from phage phi 149. Significant hybridisation was observed only with tRNA from phage phi 149-infected cells. This strongly suggests that infection of classical vibrio with phage phi 149 results in the synthesis of phage-specific tRNA molecules.
D001435:Bacteriophages / Q000378:metabolism*; D004279:DNA, Viral; D009693:Nucleic Acid Hybridization; D012343:RNA, Transfer / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*; D014734:Vibrio cholerae
D004279:DNA, Viral; D012367:RNA, Viral; D012343:RNA, Transfer