Synergism of T lymphocyte subsets in the response to Mls-locus coded antigens during graft-versus-host reaction.
After transplantation of lymphoid cells into lethally irradiated (semi)allogeneic mice specific anti-host directed effector T cells are generated. This can be demonstrated using a delayed type hypersensitivity (DTH) assay. In H-2 compatible combinations, Mls-locus antigens, but no other minor histocompatibility antigens, can induce the generation of such effector T cells. This paper shows that maximal anti-host DTH responses are obtained when the lymphoid cells transplanted constitute of a mixture of long-lived, recirculating T2 cells and short-lived, sessile T1 cells. It was demonstrated that anti-Mls locus-directed DTH effector T cells are the progeny of T2 cells, and that T1 cells amplify this response. The latter, however, are by themselves incapable of displaying anti-Mls DTH reactivity. The T1 cells were found to be of the Lyt-1+2+ phenotype, and the T2 cells of the Lyt-1+2- phenotype. The same Lyt phenotypes were found for T1 and T2 cells synergizing in the GvH reaction against H-2 alloantigens.
D000818:Animals; D002465:Cell Movement; D005260:Female; D006087:Graft vs Host Reaction; D006649:Histocompatibility Antigens / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007518:Isoantibodies; D008198:Lymph Nodes / Q000166:cytology; D008211:Lymphocyte Cooperation; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D008912:Minor Histocompatibility Loci; D010641:Phenotype; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D006649:Histocompatibility Antigens; D007518:Isoantibodies; C029805:Lyt antibodies