A sensitive and specific stable isotope assay for warfarin and its metabolites.
A capillary gas chromatographic mass spectrometric method for the quantification of warfarin and its known metabolites from microsomal incubations is described. Deuterium labelled 4', 6-, 7- and 8-hydroxy warfarins are used as internal standards and the method has detection limits of 1 ng ml-1 with 20 ng ml-1 being the lower limit for accurate quantification.
D000818:Animals; D001711:Biotransformation; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D066298:In Vitro Techniques; D007700:Kinetics; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D013237:Stereoisomerism; D014859:Warfarin / Q000032:analysis*
D014859:Warfarin