Time of vasopressin neuron origin in the mouse hypothalamus: examination by combined technique of immunocytochemistry and [3H]thymidine autoradiography.
A time-course study on production of vasopressin neurons (VP-neurons) in the mouse hypothalamus was carried out by application of [3H]thymidine autoradiography and PAP-immunocytochemistry simultaneously on the same tissue sections. In the paraventricular nucleus (PVN) and the supraoptic nucleus (SON), heavily [3H]thymidine-labeled VP-neurons were detected only in the specimens of the animals exposed to the isotope on the gestational day 12. While in the suprachiasmatic nucleus (SCN), heavily isotope-labeled VP-neurons were observed in the specimens of the animals exposed to the isotope on the gestational day 12 or day 14. Therefore, the production of the VP-neurons in the SCN is prolonged and slightly more delayed than that in the SON or PVN.
D000818:Animals; D002454:Cell Differentiation; D007032:Hypothalamus, Anterior / Q000196:embryology*; D051379:Mice; D008813:Mice, Inbred ICR; D010286:Paraventricular Hypothalamic Nucleus / Q000196:embryology / Q000378:metabolism; D013493:Suprachiasmatic Nucleus / Q000196:embryology / Q000378:metabolism; D013495:Supraoptic Nucleus / Q000196:embryology / Q000378:metabolism; D014667:Vasopressins / Q000096:biosynthesis*
D014667:Vasopressins