Immunoelectrophoretic study of the effect of radiation on protein mixtures.
This paper demonstrates the suitability of immunoelectrophoretic methods in studies of radiation effects on protein mixtures. Single serum proteins (IgG, albumin, transferrin), albumin-transferrin mixtures and human serum were irradiated with doses of 3-52 kGy. The irradiated proteins were analysed using rocket-, crossed-, crossed-with-intermediate-gel, and fused-rocket immunoelectrophoretic methods. Using crossed immunoelectrophoresis with intermediate gel, complex formation between albumin and transferrin was observed. Gel filtration monitored by fused-rocket immunoelectrophoresis was demonstrated to be a most promising method for studying protein degradation and hybrid formation.
D001798:Blood Proteins / Q000528:radiation effects; D003037:Cobalt Radioisotopes; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D007074:Immunoglobulin G / Q000528:radiation effects; D010315:Particle Accelerators; D055503:Protein Multimerization / Q000528:radiation effects; D011506:Proteins / Q000528:radiation effects*; D012709:Serum Albumin / Q000528:radiation effects; D014168:Transferrin / Q000528:radiation effects
D001798:Blood Proteins; D003037:Cobalt Radioisotopes; D007074:Immunoglobulin G; D011506:Proteins; D012709:Serum Albumin; D014168:Transferrin