title: Normal human B cells express endogenous sheep erythrocyte (E) receptor after appropriate in vitro culture.
abstract: When normal human B cells from blood, tonsil or spleen are cultured with 20-30% autologous or allogeneic T cells and mitogen, the majority of cells rosette with sheep erythrocytes (E) after 3-5 days in culture. These E+ cells are of transformed appearance and several points indicate that many are derived from B cells. Some simultaneously possess surface immunoglobulin (sIg) or a B cell antigen [detected with BA1 monoclonal antibody (mAb)] and E receptor. Many do not stain with anti-T cell mAb (UCHT1, OKT4, OKT8), while cultured T cells continue to express these antigens. Furthermore, many E+ cells appear when the original T cells have been irradiated. All the cultured E+ cells stain with OKT11, an mAb against the E receptor, and their positivity cannot therefore be attributed to mitogen-induced nonspecific stickiness. The E positivity of the B cells was shown to be endogenous since passive acquisition of E receptor shed by T cells was excluded in a number of ways: no phenotypic changes were observed when supernatants from cultures containing many E+ sIg+ non-T cells were added to B cells, or when the B and T cells were separated by a Millipore membrane; and E receptor was re-expressed after stripping with trypsin or pronase. The intimate presence of T cells is essential for the expression of E receptors by B cells, and this helper capacity was shown to reside within the OKT4-defined helper T cell subpopulation. The significance of the expression of E receptor by B cells is discussed in relation to the recent in vitro and in vivo demonstration of E+ sIg+ cells in certain leukemias and in relation to the specificity of E rosetting as a marker of T cells.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D002478:Cells, Cultured; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D014066:Palatine Tonsil / Q000276:immunology; D011971:Receptors, Immunologic / Q000276:immunology*; D012756:Sheep; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D011971:Receptors, Immunologic