[Energy-dependent inhibition of mitochondrial NAD.H-oxidase and succinic oxidase by strong nitrous bases].
The inhibitory effect of organic cations from the group of hydrazones on phosphorylated respiration in mitochondria with a succinate as a substrate has been shown to be completely reversed by 2,4-dinitrophenol (DNP). The effect observed has not been due to the action directly on the ATP-synthetase because the inhibition of ATPase, NADH-succinate oxidase and ascorbate oxidase activities occurs at a wide concentration range of compounds studied. The concentration of organic cations used for the inhibition of the respiration in submitochondria particles (SMP) as compared with respiration in mitochondria is higher; in case of SMP the inhibitory effect is not reversed by DNP. These results allow to conclude that the high inhibitory effect of organic cations is due to the energy-dependent binding of these compounds to the enzyme in the membrane phase of mitochondria.
D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects*; D006835:Hydrazones / Q000494:pharmacology*; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors*; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D051381:Rats; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors*; D014475:Uncoupling Agents / Q000494:pharmacology*
D006835:Hydrazones; D009097:Multienzyme Complexes; D014475:Uncoupling Agents; D013385:Succinate Dehydrogenase; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases
Energozavisimoe ingibirovanie NAD.H-oksidazy i suktsinatoksidazy mitokhondriĭ sil'nymi azotistymi osnovaniiami.