Determination of immunoglobulin class by pattern of absorption to protein A-sepharose coupled with immunoglobulin class specific antibodies.
The pattern of absorption of the different major immunoglobulin classes by protein A and protein A coupled with antibody to human IgA or IgM has been evaluated as a simple means of identifying the class of a particular immunoglobulin in small volumes of human serum. Rabbit anti-IgA or anti-IgM coupled with protein A-Sepharose gel effectively removed IgA or IgM respectively from human serum in addition to the subclasses of IgG removed by protein A alone. The manner in which an immunoglobulin-related property is removed from human serum by protein A and its conjugates with specific antibodies can thus serve as an index of the class of that particular immunoglobulin.
D000042:Absorption; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology / Q000378:metabolism; D007075:Immunoglobulin M / Q000276:immunology / Q000378:metabolism; D007136:Immunoglobulins / Q000145:classification* / Q000276:immunology; D011817:Rabbits; D012685:Sepharose / Q000378:metabolism*; D013205:Staphylococcal Protein A / Q000378:metabolism*
D000888:Antibodies, Anti-Idiotypic; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D007136:Immunoglobulins; D013205:Staphylococcal Protein A; D012685:Sepharose