Comparative study of the tightly bound nonhistone chromosomal proteins of rat liver and Ehrlich ascites tumour cells.
Nuclei from rat liver and Ehrlich ascites tumour (EAT) cells were fractionated into nuclear matrix and soluble chromatin. The tightly bound nonhistone chromosomal (NHC) proteins of the corresponding chromatin fractions were isolated and analyzed by means of SDS-polyacrylamide gel electrophoresis. The comparison of the electrophoretic profiles revealed that these tightly bound proteins consisted of few major fractions common to both rat liver and EAT cells.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis*; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification*; D004247:DNA / Q000302:isolation & purification; D004273:DNA, Neoplasm / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000032:analysis*; D051379:Mice; D009363:Neoplasm Proteins / Q000302:isolation & purification*; D051381:Rats
D002868:Chromosomal Proteins, Non-Histone; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D004247:DNA