A non-clonogenic assay for the determination of the sensitivity of leukemic cells to chemotherapeutic agents.
A new method for measuring the effects of chemotherapeutic agents on leukemic cells has been developed. In this method tritiated thymidine [( 3H]-TdR) is used to determine the surviving proportion of leukemic cells which are stimulated to proliferate by colony-stimulating factor (CSF). The effects of four antileukemia drugs on bone marrow cells from 20 AML patients were studied using this method and the results correlated well with the effects of the drugs on the CFUc, which were studied simultaneously. Using the liquid culture method drug effects were measured on five bone marrow specimens which failed to clone in the CFUc assay as well as on three specimens which produced too few colonies/clusters to allow estimation of drug effects.
D015250:Aclarubicin; D000609:Aminoacridines / Q000494:pharmacology; D000677:Amsacrine; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D003561:Cytarabine / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D008722:Methods; D009279:Naphthacenes / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014410:Tumor Stem Cell Assay
D000609:Aminoacridines; D000970:Antineoplastic Agents; D003115:Colony-Stimulating Factors; D009279:Naphthacenes; D000677:Amsacrine; D003561:Cytarabine; D015250:Aclarubicin; D004317:Doxorubicin; D013936:Thymidine