The effect of neuraminidase on the relative surface charge-associated properties of rat red blood cells of different ages.
Approx. 70% of the sialic acid on the rat erythrocyte surface is susceptible to cleavage by neuraminidase (Vibrio cholerae). Neuraminidase treatment results in a reduction in the partition coefficient (K) of the red cells in a charged dextran-poly(ethylene glycol) aqueous phase system and in the electrophoretic mobility of the cells. Countercurrent distribution of rat neuraminidase-treated erythrocytes, containing 59Fe-labeled mature red cells of distinct age, indicates that (a) the electrophoretic mobilities of red cells in different cavities along the extraction train increase with increasing K, as is the case with untreated erythrocytes, and (b) the cell age-related differences in surface charge-associated properties are neither eliminated nor altered by the enzyme action.
D000818:Animals; D003377:Countercurrent Distribution; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000378:metabolism*; D008297:Male; D009439:Neuraminidase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012794:Sialic Acids / Q000097:blood; D013499:Surface Properties; D014734:Vibrio cholerae / Q000201:enzymology
D012794:Sialic Acids; D009439:Neuraminidase