title: Analysis of the temperature-dependent temporal pattern of heat-shock-protein synthesis in fish cells.
abstract: Continuous exposure of Chinook salmon embryo cells to an elevated incubation temperature of 24 degrees C induces the transient expression of a set of heat-shock or stress proteins whereas maintenance of the cells at a higher incubation temperature of 28 degrees C produces a continuous synthesis of these stress proteins. In vitro translation studies suggest that the temperature-dependent temporal pattern of stress-protein synthesis is correlated with the levels of stress-protein mRNA. This was verified using a recombinant-DNA probe complementary to the 70K heat-shock-protein mRNA. A transient increase in the level of the fish heat-shock 70K mRNA was observed in RNA samples isolated from cells continuously exposed at 24 degrees C. However, a constant increase in the level of this specific mRNA was found in RNA preparations obtained from cells maintained at 28 degrees C. Therefore, the temperature-dependent pattern of fish heat-shock-protein synthesis appears to be directly related to the level of heat-shock-protein mRNA.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004625:Embryo, Nonmammalian; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D012474:Salmon / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
chemical_list: D006360:Heat-Shock Proteins; D012333:RNA, Messenger