title: Antibodies to Dermatophagoides pteronyssinus allergen Dpt 12 contaminating rabbit antisera to human and mouse anti-immunoglobulins.
abstract: Antibodies to the allergen Dpt 12 from Dermatophagoides pteronyssinus have been demonstrated in commercially obtained rabbit anti-human IgE, IgG and IgA antisera and in a commercially obtained rabbit anti-mouse IgG1 antiserum using either double or triple antibody radioimmunoassays. Where measurable, the titre of anti-Dpt 12 antibodies varied both between different anti-isotypes and between batches of the same anti-isotype as judged by the dilution of antiserum required to bind 50% of the radiolabelled Dpt 12. The titres ranged from 1/23-1/360. In contrast, a specific rabbit anti-Dpt antiserum gave a titre of 1/4500.
mesh_terms: D000485:Allergens / Q000032:analysis / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D039741:Antigens, Dermatophagoides; D001666:Binding Sites, Antibody; D004340:Drug Contamination; D006801:Humans; D007106:Immune Sera / Q000032:analysis; D007070:Immunoglobulin A / Q000276:immunology; D007073:Immunoglobulin E / Q000032:analysis; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008925:Mites / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay
chemical_list: D000485:Allergens; D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D039741:Antigens, Dermatophagoides; D007106:Immune Sera; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007073:Immunoglobulin E