Increasing the intracellular Na+ concentration induces differentiation in a pre-B lymphocyte cell line.
The ability of lipopolysaccharide (LPS), a polyclonal B-cell mitogen, to induce differentiation in the pre-B cell tumor line 70Z/3 can be mimicked by drugs that increase the intracellular Na+ concentration. Pharmacologically increasing the cellular Na+ content with monensin, a sodium ionophore, or ouabain, a specific inhibitor of Na+, K+-ATPase, induces surface IgM expression in these cells. We have shown that LPS stimulates uptake of Na+ through an amiloride-sensitive pathway. These results show that the essential action of LPS to induce surface IgM expression is activation of a Na+ uptake system.
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008985:Monensin / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism*; D013160:Splenic Neoplasms / Q000503:physiopathology
D008070:Lipopolysaccharides; D000584:Amiloride; D008985:Monensin; D012964:Sodium