Neuronal subpopulations in cat retina which accumulate the GABA agonist, (3H)muscimol: a combined Golgi and autoradiographic study.
Golgi impregnation techniques were combined with electron microscopic autoradiography of (3H-muscimol in order to provide morphological identification of labeled neurons in the cat retina. This gamma-aminobutyric acid (GABA) agonist has been shown to label the same neurons which accumulate (3H)GABA. Selected cells were photographed and drawn by the aid of a camera lucida drawing tube prior to being thin sectioned for autoradiography. The (3H)muscimol-accumulating neurons which were identified include an interplexiform cell, five classes of conventional amacrine cell, and another cell with its soma located in the ganglion cell layer which is either a ganglion cell or a displaced amacrine. The conventional amacrine cells were compared with the recent morphological classification of Kolb et al. (Vision Res. 21: 1081-1114, '81) and identified as A2, A10, A13, A17, and A19 amacrine cells. These cells exhibit a widespread distribution providing input to all five strata of the inner plexiform layer.
D000818:Animals; D001345:Autoradiography; D002415:Cats; D005968:Glutamate Decarboxylase / Q000378:metabolism; D009118:Muscimol / Q000378:metabolism*; D010080:Oxazoles / Q000378:metabolism*; D012160:Retina / Q000166:cytology* / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D010080:Oxazoles; D009118:Muscimol; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase