Separation of dolichol and dolichyl-P in microsomal and lysosomal compartments of hepatocytes.
The distribution, labeling and interrelationship of microsomal and lysosomal dolichol and dolichyl-P in rat liver was investigated. After membrane induction with phenobarbital, N-nitrosodiethylamine and diethylhexylphthalate, the amount of microsomal and lysosomal dolichols are modulated independently. Liposomal labeled dolichol injected into the portal vein appears only in lysosomes and even after 8 days is still limited to the lysosomes. After in vivo labeling with [3H]mevalonate, high initial labeling of dolichol and dolichyl-P is present in microsomes and the labeling in microsomes is greater than that in lysosomes even after 8 h. The results demonstrate compartmentalization of the intracellular dolichols in hepatocytes. These lipids may have independent roles at different membrane locations.
D000818:Animals; D004051:Diethylhexyl Phthalate / Q000494:pharmacology; D004052:Diethylnitrosamine / Q000494:pharmacology; D004224:Diterpenes / Q000302:isolation & purification*; D004288:Dolichol Phosphates / Q000302:isolation & purification* / Q000378:metabolism; D004286:Dolichols / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008247:Lysosomes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology; D011106:Polyisoprenyl Phosphates / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains
D004224:Diterpenes; D004288:Dolichol Phosphates; D004286:Dolichols; D011106:Polyisoprenyl Phosphates; D004052:Diethylnitrosamine; D004051:Diethylhexyl Phthalate; D010634:Phenobarbital