Polyamines in Trichomonas vaginalis.
Trichomonas vaginalis was grown in a modified Bushby's medium and putrescine, spermidine and spermine levels were determined in extracts from 24- and 48-h cultures and also in the culture media. All three polyamines were present in T. vaginalis extracts; the putrescine level and putrescine/spermidine ratio were much higher than those reported for other protozoa or for mammalian tissues. There were no significant differences between 24-h and 48-h amine levels per mg protein in these extracts, but amine levels per cell were higher at 24 than at 48 h. The spent culture media had a much higher putrescine content than corresponding uninoculated media and it was concluded that T. vaginalis secreted putrescine into the culture medium.
D000818:Animals; D056890:Eukaryota / Q000378:metabolism; D008322:Mammals / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D013045:Species Specificity; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D014246:Trichomonas vaginalis / Q000378:metabolism*
D011073:Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine