title: Amino acid sequence of rat liver thiol proteinase inhibitor.
abstract: The complete amino acid sequence of rat liver thiol proteinase inhibitor is presented. Its unique 98 residue sequence was derived mostly from analyses of a limited proteolysis product generated during the isolation of the protein with an active papain affinity column and of fragments produced by cleavage at arginyl bonds. The intact protein prepared with a tetrathionate inactivated ficin column has a unique amino-terminal sequence (Ac-Met-Met-Cys-). The inhibitor has no sequence homology with any proteinase inhibitor of known structure.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D008099:Liver / Q000737:chemistry*; D010206:Papain; D010446:Peptide Fragments / Q000032:analysis; D011480:Protease Inhibitors / Q000302:isolation & purification*; D051381:Rats
chemical_list: D010446:Peptide Fragments; D011480:Protease Inhibitors; D010206:Papain