Survival of HRP-labeled spinal motoneurons of chick embryo in tissue and cell cultures.
Motoneurons in the lumbar spinal cord of chick embryos 6 days old, the age when their cell death starts, were labeled in vivo by retrograde axonal transport of horseradish peroxidase (HRP) and then cultured as cord fragments or isolated cells. In Eagle's minimum essential medium with no additives, spinal cord cells survived well, but among them, the survival of HRP-labeled motoneurons was poor. Muscle-conditioned medium and a mixture of horse serum (HS) and whole chick embryo extract (CEE) promoted motoneuron survival in both suspension culture of spinal cord fragments and monolayer culture of spinal cord cells. It appeared that multiple factors may be involved in the increased extent of motoneuron survival, because HS or CEE alone showed only weak activity in contrast with the combination of HS and CEE.
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D002642:Chick Embryo / Q000502:physiology*; D046508:Culture Techniques; D006735:Horseradish Peroxidase; D009046:Motor Neurons / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology*
D006735:Horseradish Peroxidase