title: Values of the U.S. National Reference Serum for human antibodies to native DNA obtained with commercial immunoassays for anti-DNA in systemic lupus erythematosus.
abstract: The Arthritis Foundation and the Centers for Disease Control have recently prepared a "U.S. National Reference Serum" for human antibodies to native DNA. We tested this serum with 13 commercial assays for antibodies to native DNA, to permit comparisons of the values obtained in each test. Titers ranged from 10 to 2560 in Crithidia luciliae immunofluorescence assays. The serum produced 794 int. units/mL in the Cordis ELISA assay, 136 Amersham units/mL in a radioimmunoassay, and 88 FIAX units in a fluorometric immunoassay. These results can be used for interlaboratory comparisons of differing methodologies for measuring anti-DNA.
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis*; D003421:Crithidia; D004247:DNA / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008433:Mathematics; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D012015:Reference Standards
chemical_list: D000974:Antibodies, Antinuclear; D011933:Reagent Kits, Diagnostic; D004247:DNA