title: A new crystalline modification of the copper enzyme ascorbate oxidase.
abstract: The copper enzyme ascorbate oxidase, purified from green zucchini squash, has been crystallized at pH 5.4 employing the organic solvent 2-methyl-2,4-pentanediol. The crystals obtained are larger than one millimetre and belong to the space group P2(1)2(1)2, with unit cell parameters; a = 106.7 A, b = 105.1 A, c = 113.5 A. The crystallographic asymmetric unit contains two subunits of the enzyme (Mr = 140,000) and the solvent content of the crystals is 46% (v/v). The diffraction pattern extends to 2.5 A resolution; this crystal form is suitable for a X-ray structural investigation.
mesh_terms: D001204:Ascorbate Oxidase; D003460:Crystallization; D010088:Oxidoreductases; D010944:Plants / Q000201:enzymology; D014961:X-Ray Diffraction
chemical_list: D010088:Oxidoreductases; D001204:Ascorbate Oxidase