Effect of glucagon, phenylephrine, and isoproterenol on glycogenolysis and glucose release from fetal rat hepatocytes in suspension.
Fetal hepatocytes isolated at day 22 of gestation by trypsin digestion of rat liver retain their responsiveness to hormones. Glucagon significantly stimulates glycogenolysis and glucose release to 115 and 124%, respectively. The effect of alpha- and beta-agonists is more pronounced enhancing glycogen breakdown and glucose release to 133 and 147% (L-phenylephrine) and 183 and 202% (isoproterenol), respectively. The isolated fetal hepatocytes obtained by trypsin digestion are a useful tool for studying the hormonal control of liver metabolism during the perinatal stage without limitations met otherwise with extrahepatic factors and the effect of altered blood flow.
D000818:Animals; D005333:Fetus; D005934:Glucagon / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008112:Liver Glycogen / Q000378:metabolism*; D010656:Phenylephrine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D008112:Liver Glycogen; D010656:Phenylephrine; D005934:Glucagon; D005947:Glucose; D007545:Isoproterenol