An inexpensive filtration unit and manifold for radioligand binding studies.
An inexpensive filtration manifold for radioligand binding studies is described. The manifold can be for any number of filter units, is inexpensive to produce, and is easy to use. The filter units are produced from Swinnex 25-mm syringe filter units. The radioligand binding curve for [3H] QNB to guinea pig ventricle membrane fraction shows that the unit produces consistent results.
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D005260:Female; D005374:Filtration / Q000295:instrumentation*; D006168:Guinea Pigs; D008566:Membranes / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D011485:Protein Binding; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D011869:Radioligand Assay / Q000295:instrumentation*
D011813:Quinuclidinyl Benzilate; D001285:Atropine