[The properties of precipitation reactions between secretion products in the oviduct of pleurodeles: identification of a lectin].
The properties of two precipitation reactions occurring between secretory products from the oviduct of Pleurodeles waltl have been studied. It has been demonstrated that a lectin is involved in one of the reactions. This lectin precipitated glycogen and starch and required calcium; the most potent saccharide inhibitors were 2-amino-2-deoxy-D-glucose and D-glucose, respectively. The other reaction was related to glycoproteins (probably sulfated glycoproteins) that contained sulphur. The properties of this reaction were not the same as purely ionic interactions; basic protein-acidic polysaccharide interactions have been compared. A lectin was probably implicated but this could not be demonstrated because no saccharide inhibitor was found. There are several similitudes between this reaction and the lectin-galactoside reaction which occurs in the reaction between cortical granule content and egg jellies in anurans.
D000818:Animals; D011232:Chemical Precipitation; D005260:Female; D006003:Glycogen / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D037102:Lectins / Q000302:isolation & purification*; D010057:Oviducts / Q000276:immunology / Q000378:metabolism*; D010999:Pleurodeles / Q000502:physiology*; D012455:Salamandridae / Q000502:physiology*; D013213:Starch / Q000378:metabolism
D006023:Glycoproteins; D037102:Lectins; D013213:Starch; D006003:Glycogen
Caractéristiques des réactions de précipitation entre les produits de sécrétion de l'oviducte du Pleurodèle: identification d'une lectine.