Phorbol esters inactivate the lytic apparatus of cytotoxic T lymphocytes.
Treatment of cloned murine CTL with phorbol myristate acetate (PMA) initiates the loss of lytic potential of these cells. The process is relatively rapid (t 1/2 = 2.2 +/- 0.7 hr), occurs independently of protein synthesis, and can be largely reversed within 3 hr by incubation in normal medium with or without the addition of supernatants from activated lymphocytes. Examination of PMA-treated or untreated CTL in assays that separate binding and post-binding events suggests that the lytic apparatus itself is a major target of the PMA-initiated event.
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D010704:Phorbols / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects* / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D010704:Phorbols; D013755:Tetradecanoylphorbol Acetate