Human B lymphocytes show greater susceptibility to H2O2 toxicity than T lymphocytes.
Lymphocytes from patients with chronic lymphocytic leukemia (CLL) and from normal subjects were incubated with a glucose-glucose oxidase hydrogen peroxide (H2O2) generating system to study the effect of oxidant stress on these cells. Within 4 hr, 90% of normal but only 21% of CLL lymphocytes remained viable. When normal and CLL preparations enriched in B or T cells were exposed to H2O2, B lymphocytes from both groups were highly susceptible to oxidative damage while T lymphocytes were relatively resistant. The H2O2 scavenger catalase prevented the cytotoxicity. The present work identifies the human B lymphocyte as a cell that should be a suitable target for selective killing by H2O2-generating systems.
D001402:B-Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D002374:Catalase / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D005949:Glucose Oxidase / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology*; D007945:Leukemia, Lymphoid / Q000097:blood*; D010101:Oxygen Consumption / Q000187:drug effects; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology
D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D002374:Catalase