Depletion of murine anti-azobenzenearsonate plaque-forming cells by derivatized polyacrylamide beads.
The depletion of secondary p-azobenzenearsonate plaque-forming cells (ABA-PFC) by affinity columns substituted with ABA was dependent on the primary dose, times elapsed after priming, and the nature of the side-arm on the columns. Thus, higher priming doses of ABA coupled to keyhole limpet haemocyanin (50-500 micrograms of ABA-KLH in complete Freund's adjuvant (CFA] favoured depletion of ABA-PFC by ABA coupled to a 6- aminocaproyltyrosine side-arm (SAC-TYR-ABA beads), whereas ABA-PFC from mice primed with 1 microgram of ABA-KLH in CFA were never depleted; this latter population were only depleted by ABA coupled to a histamine side-arm (HIST-ABA beads) 7 months after priming. These data are consistent with the notion that two specificities, HIST-ABA and TYR-ABA, appear to emerge independently, lower priming doses inducing the preferential appearance of the HIST-ABA specificity.
D000180:Acrylic Resins / Q000276:immunology; D000818:Animals; D000915:Antibody Affinity; D000917:Antibody Formation; D001391:Azo Compounds / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006241:Haptens; D007156:Immunologic Memory; D008297:Male; D051379:Mice; D013997:Time Factors; D010132:p-Azobenzenearsonate / Q000008:administration & dosage / Q000276:immunology*; D000099166:Polyacrylamides
D000180:Acrylic Resins; D001391:Azo Compounds; D006241:Haptens; D010132:p-Azobenzenearsonate; D000099166:Polyacrylamides