Inhibition of glutamate dehydrogenase activity in rabbit renal mitochondria by phosphoenolpyruvate.
The effect of phosphoenolpyruvate on glutamate dehydrogenase activity was studied in both intact and Triton X-100-treated rabbit renal mitochondria. The intramitochondrial phosphoenolpyruvate content was modulated by application of both 3-MPA, an inhibitor of phosphoenolpyruvate carboxykinase, and BTCA, which inhibits the tricarboxylate-transporting system. The data indicate that: (i) phosphoenolpyruvate is a potent inhibitor of glutamate dehydrogenase activity; and (ii) its inhibitory effect on the enzyme may be abolished by leucine and ADP, activators of glutamate dehydrogenase.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology; D055598:Chemical Phenomena; D002621:Chemistry; D005969:Glutamate Dehydrogenase / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D007668:Kidney / Q000201:enzymology*; D007930:Leucine / Q000494:pharmacology; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D010728:Phosphoenolpyruvate / Q000494:pharmacology*; D010848:Picolinic Acids / Q000494:pharmacology; D011817:Rabbits; D014233:Tricarboxylic Acids / Q000494:pharmacology
D001555:Benzene Derivatives; D010848:Picolinic Acids; D014233:Tricarboxylic Acids; C004288:benzene 1,2,3-tricarboxylic acid; D000244:Adenosine Diphosphate; D010728:Phosphoenolpyruvate; C008363:3-mercaptopicolinic acid; D005969:Glutamate Dehydrogenase; D007930:Leucine