Enzyme-linked immunoassay for human granulocyte elastase in complex with alpha 1-proteinase inhibitor.
Granulocyte elastase in complex with its main inhibitor in plasma, i.e. alpha 1-proteinase inhibitor, was quantitatively determined by incubating the sample with solid-phase fixed antibodies against elastase first and reacting then with alkaline phosphatase-labelled antibodies against alpha 1-proteinase inhibitor. In normal plasma a level of 97.5 +/- 25.8 micrograms elastase/1 (mean +/- s.d., n = 43) was found, whereas moderately to markedly increased plasma concentrations were demonstrated in a variety of patients with inflammatory diseases like septicemia or rheumatoid arthritis.
D000368:Aged; D001172:Arthritis, Rheumatoid / Q000201:enzymology*; D001798:Blood Proteins / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007249:Inflammation; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000097:blood* / Q000302:isolation & purification; D011480:Protease Inhibitors / Q000378:metabolism*; D011485:Protein Binding; D012016:Reference Values; D018805:Sepsis / Q000201:enzymology*; D000515:alpha 1-Antitrypsin
D001798:Blood Proteins; D011480:Protease Inhibitors; D000515:alpha 1-Antitrypsin; D010196:Pancreatic Elastase