Cell surface antigens and other characteristics of T cells regulating the antibody response to type III pneumococcal polysaccharide.
The administration of a subimmunogenic dose of type III pneumococcal polysaccharide (SSS-III) produces an antigen-specific T cell-dependent phenomenon termed low-dose paralysis (immunologic unresponsiveness). This form of unresponsiveness can be transferred by spleen cells obtained 5 to 24 hr after priming, and the suppressive activity of the transferred cells is abolished by prior treatment with monoclonal anti-Lyt-2 and anti-I-J antibody in the presence of complement, indicating that suppression is mediated by a distinct subset of T cells (suppressor T cells). If primed spleen cells are transferred 24 to 72 hr after immunization with SSS-III, however, the resulting antibody response of immunized recipients is enhanced. Greater enhancement is noted when transferred cells, pretreated with monoclonal anti-Lyt-2 antibody plus complement to remove suppressor T cells, are used; such enhancement is attributed to amplifier T cells. These findings indicate suppressor T cells regulate the antibody response to SSS-III by influencing the expansion of SSS-III-specific clones of B cells as well as the expression of amplifier T cell activity; the latter causes B cells to proliferate further in response to SSS-III.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000950:Antigens, Ly / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D003348:Cortisone / Q000494:pharmacology; D003520:Cyclophosphamide / Q000494:pharmacology; D005260:Female; D000949:Histocompatibility Antigens Class II; D007108:Immune Tolerance / Q000187:drug effects; D051379:Mice; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D013154:Spleen / Q000276:immunology / Q000528:radiation effects; D013296:Streptococcus pneumoniae / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000950:Antigens, Ly; D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D011135:Polysaccharides, Bacterial; D003520:Cyclophosphamide; D003348:Cortisone