Effect of various immunomodulators on the production of granulocyte/macrophage colony-stimulating factor (GM-CSF) in mouse spleen cell cultures.
Among various immunomodulating agents examined, OK-432 (Streptococcus pyogenes cell bodies), BCG (bacillus Calmette-Guerin) and LPS (lipopolysaccharide) were effective in enhancing the production of granulocyte/macrophage colony-stimulating factor (GM-CSF) in the cultures of mouse spleen cells. Control non-stimulated spleen cell cultures accumulated GM-CSF activity maximally on day 3 and lost it subsequently within a few days. On the other hand, a large amount of GM-CSF activity could be detected in the medium until day 9 when OK-432 was added in the culture at the start of incubation. The GM-CSF obtained from OK-432 or LPS stimulated cultures and the GM-CSF obtained from the control non-stimulated culture eluted from an Ultro-gel AcA 34 column at a similar position and inactivated similarly at 80 degrees C. However, these three GM-CSFs behaved in slightly different manners upon isoelectrofocusing. These results suggest that the GM-CSFs produced in increasing amounts by the stimulated spleen cells are similar to that produced by the control non-stimulated cells, but slightly different from one another in the extent of sialylation according to the difference of the stimulant.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D006098:Granulocytes; D007525:Isoelectric Focusing; D008264:Macrophages; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D013154:Spleen / Q000378:metabolism*
D000276:Adjuvants, Immunologic; D003115:Colony-Stimulating Factors