The growth of cultured human foreskin keratinocytes is not stimulated by a tumor promoter.
The tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA) does not stimulate the growth of human epidermal cells in foreskin explant cultures; a dose-dependent inhibition is seen at doses higher than 10(-5) micrograms/ml. TPA also inhibits epidermal growth factor-stimulated growth and does not induce ornithine decarboxylase activity or increase polyamine levels. This is not due to the rapid breakdown of TPA, since TPA is not metabolized to any appreciable extent.
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006801:Humans; D008297:Male; D009955:Ornithine Decarboxylase / Q000378:metabolism; D010704:Phorbols / Q000494:pharmacology*; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D010704:Phorbols; D004815:Epidermal Growth Factor; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate