Detection of differences in surface carbohydrates of unfertilized and fertilized golden hamster eggs using potato agglutinin.
Changes in the surface carbohydrate moieties of zonae pellucidae and naked vitelli isolated from unfertilized and fertilized hamster eggs were examined through potato agglutinin (STA)-mediated agglutination and binding of FITC-STA as a marker. The results indicated that the stereochemical properties of the saccharide residues within STA-binding sites on the outer surface of the zona pellucida, inner region of the zona pellucida, and the surface of the ooplasmic membrane were different in unfertilized and fertilized eggs, that part of the differences between the two kinds of eggs could be visualized with FITC-STA and that, on the basis of these data, if we use STA as a probe to analyze the surface change following fertilization, we may be able to analyze these changes to a substantial degree.
D000373:Agglutinins; D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D006224:Cricetinae; D005260:Female; D005306:Fertilization; D008647:Mesocricetus; D010063:Ovum / Q000032:analysis / Q000502:physiology*; D015044:Zona Pellucida / Q000502:physiology
D000373:Agglutinins; D002241:Carbohydrates