Anti-idiotypic cytotoxic T cells in rats with graft-versus-host disease.
Transfer of parental strain T lymphocytes into F1 hybrid rats differing with respect to gene products of the major histocompatibility complex (MHC) causes a graft-versus-host (GvH) reaction. This reaction results from recognition of host allogeneic MHC gene products by specific clones of donor T cells. When given systemically in sufficient numbers, these donor T cells cause a progressive, generally fatal wasting syndrome, an early feature of which includes extensive splenomegaly. A more local, non-fatal GvH reaction, marked by extensive enlargement of the draining lymph nodes, ensues when donor T cells are administered via the footpad. Here, we demonstrate that cells derived from the enlarged draining lymph nodes of A/B F1 animals undergoing local GvH disease caused by donor A T cells contain a subpopulation of host-derived killer T-cell precursors which can be activated to lyse specific blast cells, derived from mixed lymphocyte culture (MLC), reactive to host MHCb alloantigens. These 'anti-idiotypic' cytolytic T cells lyse A anti-MHCb MLC blasts, and also, they lyse anti-MHCb MLC blasts from MHC different, third party rat strains.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D003602:Cytotoxicity, Immunologic; D006086:Graft vs Host Disease / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology; D008285:Major Histocompatibility Complex; D010641:Phenotype; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D007130:Immunoglobulin Idiotypes