Measurement of leucine enkephalin in caudate nucleus tissue with fast atom bombardment-collision activated dissociation-linked field scanning mass spectrometry.
The endogenous amount of the opioid pentapeptide leucine enkephalin was measured in a canine caudate nucleus tissue extract using mass spectral analytical methods which retain absolute molecular specificity. Fast atom bombardment mass spectrometry generation of the protonated molecular ion of leucine enkephalin followed by collision activated dissociation produced amino acid sequence-determining ions. These amino acid sequence-determining ions were analyzed by a linked field (B/E) scan. One amino acid sequence-determining ion was selected to measure endogenous leucine enkephalin. This novel measurement mode offers optimal molecular specificity for quantification of an endogenous amount (451 pmol g-1 tissue) of leucine enkephalin in a biologic tissue extract of canine caudate nucleus.
D000818:Animals; D002421:Caudate Nucleus / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D004743:Enkephalin, Leucine / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods
D004743:Enkephalin, Leucine