Effect of antithrombin III, alpha 1-proteinase inhibitor and heparin on amidolytic activity of nerve growth factor (7S-NGF).
Amidolysis catalysed by nerve growth factor (7S-NGF) and by a glandular kallikrein, unlike that catalysed by thrombin, was not inhibited by antithrombin III, either in the presence or absence of heparin. Inhibition by alpha 1-proteinase inhibitor of thrombin-, but not of 7S-NGF- or kallikrein-catalysed amidolysis was alleviated by incubation of enzyme and heparin before addition of inhibitor. These data are discussed in terms of a possible control of growth factor activity by antiproteinases and glycosaminoglycans.
D000818:Animals; D000990:Antithrombin III / Q000494:pharmacology*; D001798:Blood Proteins / Q000494:pharmacology*; D002417:Cattle; D004151:Dipeptides / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology*; D007610:Kallikreins / Q000378:metabolism; D051379:Mice; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009842:Oligopeptides / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology; D000515:alpha 1-Antitrypsin
D001798:Blood Proteins; D004151:Dipeptides; D009414:Nerve Growth Factors; D009842:Oligopeptides; D000515:alpha 1-Antitrypsin; C018788:valyl-leucyl-lysine 4-nitroanilide; C021129:S 2238; C031006:Val-Leu-Arg-p-nitroanilide; D000990:Antithrombin III; D006493:Heparin; D007610:Kallikreins; D013917:Thrombin