Uptake and retention of moulting hormones by the integument of crayfishes in vitro. II. Influence of metabolic inhibitors and sulphydryl group inhibitors.
Antimycin A and 2,4-dinitrophenol reduced both the initial uptake and the total uptake of ecdysone into crayfish hypodermis in vitro. Antimycin A did not reduce the retention of moulting hormone, in contrast to 2,4-dinitrophenol. The inhibitory effect of antimycin A could be completely overcome. The membrane-mediated ecdysteroid uptake was sensitive to the sulphydryl group inhibitors N-ethylmaleinimide (NEM) and p-chloromercuriphenylsulphonate (PCMPS). Inhibition of steroid uptake by PCMPS was completely reversed by dithiothreitol.
D019297:2,4-Dinitrophenol; D000818:Animals; D000968:Antimycin A / Q000494:pharmacology*; D003400:Astacoidea / Q000378:metabolism*; D004140:Dinitrophenols / Q000494:pharmacology*; D004440:Ecdysone / Q000378:metabolism*; D066298:In Vitro Techniques; D010101:Oxygen Consumption / Q000187:drug effects; D012867:Skin / Q000378:metabolism; D013439:Sulfhydryl Reagents / Q000494:pharmacology*
D004140:Dinitrophenols; D013439:Sulfhydryl Reagents; D004440:Ecdysone; D000968:Antimycin A; D019297:2,4-Dinitrophenol