Absence of detectable cytotoxic lymphocytes in the spleens of guinea pigs immunized with allogeneic cells.
Using inbred guinea pigs of strains JY 1, JY 2, 2 and 13, whose major histocompatibility gene complexes are different from each other at the B region and/or the I region, the authors aimed to detect cytotoxic thymus-derived lymphocytes (CTL) against allogeneic target cells. Various in vivo and in vitro sensitization methods that have been described for murine CTL systems were used. For detection of CTL, Con A- or PHA-induced 51Cr-labeled lymphoblasts were incubated with sensitized spleen cells or lymph node cells for 4 hr, and 51Cr released into the supernatant was assayed. Under these conditions, no significant lysis of the target cells was observed. On the other hand, significant levels of both delayed-type hypersensitivity and humoral antibodies were detected in the animals sensitized by in vivo methods.
D000818:Animals; D000917:Antibody Formation; D006168:Guinea Pigs; D006649:Histocompatibility Antigens; D006968:Hypersensitivity, Delayed; D007114:Immunization; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D013045:Species Specificity; D013154:Spleen / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D006649:Histocompatibility Antigens