Functional properties of lymphocytes isolated from murine small intestinal epithelium.
Intraepithelial lymphocytes (IEL) were isolated from murine small intestine using a modification of previously published procedures. Analysis of IEL by immunofluorescence using monoclonal antibodies showed they were predominantly Lyt-2+ cells, with relatively few B cells or macrophages present. IEL cultured at sufficiently high cell densities proliferated in response to concanavalin A (Con A), phytohaemagglutin (PHA) and lipopolysaccharide (LPS). IEL were also capable of recognizing alloantigens in an in vivo graft-versus-host assay and in an in vitro mixed lymphocyte reaction. These studies therefore confirm that murine IEL contain cells with immunologic properties characteristic of typical T lymphocytes.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002478:Cells, Cultured; D004848:Epithelium / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D007421:Intestine, Small / Q000276:immunology*; D007518:Isoantibodies / Q000276:immunology; D007519:Isoantigens / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology
D000954:Antigens, Surface; D007376:Interleukin-2; D007518:Isoantibodies; D007519:Isoantigens; C029805:Lyt antibodies; D008934:Mitogens