A fine structural study of the formation of temporary swellings (Herring bodies) and reversible degeneration of neurosecretory axons following microiontophoretic ejection of vinblastine into the hypothalamo-neurohypophysial tract of the frog (Rana pipiens).
The microiontophoretic ejection of vinblastine into the median eminence effectively blocked axonal transport in the hypothalamoneurohypophysial system of the frog. Proximal to the ejection site, large axon swellings (Herring bodies) were found that contained mainly abundant neurosecretory granulated vesicles and axonal smooth endoplasmic reticulum. At advanced post ejection times, autophagic events were obvious. Subsequently, preexperimental conditions were reestablished, indicative of the resumption of axonal transport. Distal to the ejection site, i.e. in the neural lobe, the relatively long block of axonal transport subsequent to the vinblastine ejection caused most axons to degenerate and to be phagocytized by pituicytes. The degenerative process was reversible since upon reestablishment of the axonal transport regeneration of the neurosecretory fibers occurred.
D000818:Animals; D001369:Axons / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D008473:Median Eminence / Q000502:physiology*; D008845:Microinjections; D008854:Microscopy, Electron; D009410:Nerve Degeneration / Q000187:drug effects*; D009490:Neurosecretory Systems / Q000502:physiology*; D011894:Rana pipiens; D014747:Vinblastine / Q000008:administration & dosage / Q000633:toxicity*
D014747:Vinblastine