Thin-layer chromatography-densitometry of minor acidic phospholipids: application to lipids from erythrocytes, liver, and kidney.
A method for quantitative determination of acidic phospholipids by thin-layer chromatography (TLC) followed by densitometry is described. The total lipids were separated into neutral and acidic fractions by DEAE-Sephadex column chromatography. A clear-cut separation of acidic phospholipids was achieved by high-performance TLC with a solvent system of chloroform/acetone/acetic acid/formic acid/water (60/60/4/10/3). Each phospholipid band was quantitated by densitometry with the use of an internal standard. The lipid compositions of sheep and mouse erythrocytes and of rat liver and kidney were determined by the present method.
D000818:Animals; D002855:Chromatography, Thin Layer; D003720:Densitometry; D004912:Erythrocytes / Q000032:analysis*; D007668:Kidney / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D008563:Membrane Lipids / Q000032:analysis; D051379:Mice; D010743:Phospholipids / Q000302:isolation & purification*; D012015:Reference Standards; D012756:Sheep; D012997:Solvents
D008563:Membrane Lipids; D010743:Phospholipids; D012997:Solvents