title: Determination of alkanes in breath to monitor lipid peroxidation in the presence of volatile toxicants and metabolites. An optimized, automatic method.
abstract: Determination of alkanes in breath of laboratory animals and humans has become a standard-method for monitoring lipid peroxidation in vivo. Isothermal gas chromatography on Porasil C enables sensitive, rapid and repetitive determination of all C2-C5-hydrocarbons in breath. Volatile toxicants and metabolites, which would coelute with the alkanes of later injected samples, are deviated by using a precolumn. An automatic switching unit controls withdrawal and injection of samples and backflush of the precolumn in a repetitive manner at fixed intervals. This increases accuracy and sensitivity of analysis and enables virtually unattended operation. The system has been applied for a study on the oxygen-dependence of CCl4-metabolism in the rat.
mesh_terms: D000473:Alkanes / Q000032:analysis*; D000818:Animals; D001944:Breath Tests / Q000295:instrumentation / Q000379:methods*; D002251:Carbon Tetrachloride / Q000378:metabolism*; D002849:Chromatography, Gas / Q000379:methods; D008054:Lipid Peroxides / Q000378:metabolism*; D010100:Oxygen; D010313:Partial Pressure; D051381:Rats
chemical_list: D000473:Alkanes; D008054:Lipid Peroxides; D002251:Carbon Tetrachloride; D010100:Oxygen