Ultraviolet irradiation of Rana pipiens embryos delays the migration of primordial germ cells into the genital ridges.
Ultraviolet (UV) irradiation of the vegetal pole of anuran embryos at the two-cell stage has been reported to cause aberrant cleavage as well as a subsequent reduction in germ cell numbers. In this study, we find no correlation between UV-induced cleavage abnormalities and the absence of primordial germ cells in Rana pipiens tadpoles examined at stage 25. On the other hand, some tadpoles from a population which was lacking primordial germ cells at stage 25 subsequently contained germ cells. These late-appearing germs cells exhibited damaged mitochondria, autophagosomes, and secondary lysosomes, while surrounding somatic cells were morphologically normal. We suggest that these cytoplasmic abnormalities resulted from an effect of the initial UV irradiation of germ plasm. We conclude that one effect of UV irradiation of germ plasm is to delay or inhibit the migration of primordial germ cells into the genital ridges.
D000818:Animals; D002455:Cell Division / Q000528:radiation effects; D002465:Cell Movement / Q000528:radiation effects; D005260:Female; D005854:Germ Cells / Q000166:cytology / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male; D011894:Rana pipiens / Q000196:embryology*; D014466:Ultraviolet Rays