title: Development of bronchus associated lymphoid tissue (BALT) in the rat, with special reference to T- and B-cells.
abstract: The development of bronchus associated lymphoid tissue was studied in normal rats, in serial sections, using both routine histological techniques and the two-step immuno-peroxidase method on cryostat sections for demonstration of T lymphocytes, IgM-, IgG- and IgA-bearing B lymphocytes and plasma cells, respectively. BALT first appears 4 days postpartum (p.p.) as a condensation of reticulum cells near a lymph vessel, between the main bronchus and the accompanying artery. Only a few lymphocytes are present at first. Vascularisation is considerable 8 days p.p. and includes high endothelial venules. Leucocytes are seen in transit in both blood- and lymph vessel walls. Lymphocytes populate the area under the epithelium from about 2 weeks p.p. A few IgM, IgG- and IgA-bearing B cells are already present at 4 days, and rapidly increase in numbers; T cells usually appear at day 8. Discrete T- and B-cell areas do not appear until 4 weeks and are only observed regularly after 12 weeks, when secondary follicles appear. BALT starts development at a similar time to other peripheral lymphoid organs but apparently achieves immunological activity later. It is concluded that antigens probably play an important part in organising BALT.
mesh_terms: D000367:Age Factors; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D001980:Bronchi / Q000166:cytology* / Q000276:immunology; D008221:Lymphoid Tissue / Q000166:cytology / Q000254:growth & development* / Q000276:immunology; D051381:Rats; D013601:T-Lymphocytes / Q000166:cytology*