GSSG-reducing activity in lenses deficient in glucose-6-phosphate dehydrogenase.
Steroids that inhibit glucose-6-phosphate dehydrogenase (G6PD) were used to examine the correlation between the loss of GSSG-reducing activity and G6PD deficiency in the lens. The correlation was found to be nonlinear. In senile cataracts, which had lost 36% of NADPH-generating activity as compared to clear lenses, the estimated loss of GSSG reduction was only 20%. On the other hand, lenses with severe G6PD deficiency (i.e. 93% loss) retained at least 28% GSSG-reducing activity. The declined reducing activity, however, suggested a possible role of G6PD deficiency in cataract formation in young patients.
D000328:Adult; D000367:Age Factors; D000818:Animals; D002386:Cataract / Q000201:enzymology* / Q000209:etiology; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis / Q000037:antagonists & inhibitors; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000201:enzymology*; D005978:Glutathione / Q000031:analogs & derivatives* / Q000378:metabolism; D019803:Glutathione Disulfide; D006801:Humans; D007904:Lens Cortex, Crystalline / Q000201:enzymology; D007908:Lens, Crystalline / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D051381:Rats; D011919:Rats, Inbred Strains; D013256:Steroids / Q000494:pharmacology
D013256:Steroids; D005954:Glucosephosphate Dehydrogenase; D005978:Glutathione; D019803:Glutathione Disulfide