[Effect of thiamine on the activity of the pyruvate dehydrogenase complex in rat liver following stimulation of lipogenesis].
It is shown that thiamine administration to rats (250 micrograms per 100 g of mass) who were given high-carbohydrate diet (lipogenesis intensification) after fasting inhibits an increase in the pyruvate dehydrogenase activity in the liver homogenate and mitochondria usual under these conditions. This is observed when determining total activity of the pyruvate dehydrogenase complex and activity of its first component--pyruvate dehydrogenase estimated from the ferricyanide reduction and [1-14C] CO2 formation from [1-14C] pyruvate. Fasting animals and animals whom thiamine was administered against a background of lipogenesis intensification revealed a higher ability of the liver tissue to synthesize acetoin as compared with the control group and animals with the intensified lipogenesis without thiamine administration.
D000093:Acetoin / Q000096:biosynthesis; D000818:Animals; D004040:Dietary Carbohydrates / Q000008:administration & dosage; D004789:Enzyme Activation / Q000187:drug effects; D005508:Food Deprivation / Q000502:physiology; D050356:Lipid Metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*; D051381:Rats; D013831:Thiamine / Q000494:pharmacology*
D004040:Dietary Carbohydrates; D011768:Pyruvate Dehydrogenase Complex; D000093:Acetoin; D013831:Thiamine
Vliianie tiamina na aktivnost' piruvatdegidrogenaznogo kompleksa pecheni krys pri intensifikatsii lipogeneza.