Activation of antigen-enriched b cells. I. Purification and response to thymus-independent antigens.
The development of a procedure for the selection of a large number of functional antigen-binding B cells is described. Forty to 70% of the cells in the enriched population bind antigen, and the antigen-binding can be inhibited by pretreatment with either the free hapten (TNP-Lys) or anti-immunoglobulin. The enriched cells express both slgM and slgD and respond to mitogenic stimuli by proliferating and developing into antibody-forming cells. In contrast to normal spleen cells, however, the enriched cells also proliferate in response to submitogenic levels of TNP-Brucella abortus and TNP-LPS.
D000818:Animals; D000955:Antigens, T-Independent / Q000276:immunology*; D001241:Aspirin / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D002478:Cells, Cultured; D008070:Lipopolysaccharides / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D012397:Rosette Formation; D014301:Trinitrobenzenes / Q000276:immunology
D000955:Antigens, T-Independent; D008070:Lipopolysaccharides; D011947:Receptors, Antigen, B-Cell; D014301:Trinitrobenzenes; D001241:Aspirin