OH radical-induced crosslinks of methionine peptides.
Reactions of radiation-generated OH radicals with methionine (Met) and its homopeptides, L-Met-L-Met and tri-L-Met, were investigated through reaction products. Samples of irradiated Met and HCl-hydrolysates of its irradiated homopeptides were trimethylsilylated and analysed by capillary gas chromatography-mass spectrometry. Mass spectra taken revealed the formation of three products, 2-amino-4-(methyldithio)butanoic acid, 4,4'-thiobis(2-aminobutanoic acid) and homocystine. G values of these products were determined to be 0.10, 0.16 and 0.30, respectively.
D003037:Cobalt Radioisotopes; D004151:Dipeptides / Q000528:radiation effects*; D005720:Gamma Rays; D008401:Gas Chromatography-Mass Spectrometry; D006878:Hydroxides; D008715:Methionine / Q000528:radiation effects*; D009842:Oligopeptides / Q000528:radiation effects*; D011854:Radiochemistry
D003037:Cobalt Radioisotopes; D004151:Dipeptides; D006878:Hydroxides; D009842:Oligopeptides; C022877:trimethionine; C034758:methionylmethionine; C031356:hydroxide ion; D008715:Methionine