title: A new site-specific endodeoxyribonuclease from Citrobacter freundii.
abstract: Cfr10 I, a site-specific endonuclease from Citrobacter freundii strain RFL10, was isolated. It recognizes and cleaves the family of related sequences: 5'Pu decreases CCGGPy to generate DNA fragments with 5' tetranucleotide extensions. Cfr10 I may be useful in molecular cloning experiments, especially in conjunction with other enzymes which generate the same terminal extensions.
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002954:Citrobacter / Q000201:enzymology*; D004269:DNA, Bacterial / Q000302:isolation & purification; D004706:Endodeoxyribonucleases / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D004706:Endodeoxyribonucleases