A study of the susceptibility of three species of primate to vaginal colonization with Gardnerella vaginalis.
In an attempt to develop an animal model of Gardnerella-associated vaginitis, several strains of Gardnerella vaginalis were inoculated into the lower genital tract of female pig-tailed macaques, tamarins and chimpanzees. G. vaginalis was not recovered from either tamarins or chimpanzees, but was recovered from each of 1O pig-tailed macaques inoculated with either of two freshly isolated Gardnerella strains, colonization persisting for 11-39 days. Examination of Gram-stained vaginal smears obtained from infected pig-tailed macaques failed to demonstrate clue cells, a feature which is pathognomonic of Gardnerella-associated vaginitis in humans. Other features characteristic of non-specific vaginitis, namely an increase in vaginal pH, and an increase in the ratio of succinate to lactate (S/L ratio) in vaginal fluid were not found. However, the physiology of the macaque vagina was found to be different from that of the human, the vaginal pH and S/L ratio of uninfected macaques both being higher than that seen in humans. The physiological differences between the macaque and human vagina may be due, in part, to a difference in their anaerobic vaginal flora. While these inter-species differences in vaginal physiology and microbiology limit the relevance of the pig-tailed macaque as a model of Gardnerella-associated vaginitis, the ease with which macaques are colonized with G. vaginalis may prove useful in studying bacterial adhesion and local immunity.
D000818:Animals; D001420:Bacteria, Aerobic / Q000302:isolation & purification; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D006194:Gardnerella vaginalis / Q000302:isolation & purification*; D006190:Haemophilus / Q000302:isolation & purification*; D006192:Haemophilus Infections / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D008254:Macaca nemestrina; D002679:Pan troglodytes; D012452:Saguinus; D014621:Vagina / Q000382:microbiology*; D014626:Vaginal Smears; D014627:Vaginitis / Q000382:microbiology
D005227:Fatty Acids