Oligomeric forms of potato spindle tuber viroid (PSTV) and of its complementary RNA are present in nuclei isolated from viroid-infected potato cells.
Different oligomeric forms of PSTV are detected in nuclei isolated from PSTV-infected potato cells by means of molecular hybridization, using as probes synthetic oligodeoxyribonucleotides with sequence specificity for (+)PSTV and for (-)PSTV. In addition to several species of longer-than-unit-length (-)PSTV molecules, two oligomeric forms of (+)PSTV are detected, which correspond in size to RNA strands of approximately two and three times viroid unit-length. They must be considered as the precursors of the circular and linear (+)PSTV monomers accumulating in the cell nucleus.
D001483:Base Sequence; D002467:Cell Nucleus / Q000032:analysis; D046911:Macromolecular Substances; D009693:Nucleic Acid Hybridization; D010942:Plant Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis*; D014772:Viroids / Q000235:genetics*
D046911:Macromolecular Substances; D012367:RNA, Viral