Accelerated 86Rb+ (K+) release from the cytotoxic T lymphocyte is a physiologic event associated with delivery of the lethal hit.
Cloned CTL have been loaded with the K+ analogue 86Rb+. When mixed with unlabeled target cells, accelerated 86Rb+ release occurs from the CTL. The magnitude of accelerated 86Rb+ release is proportional to the efficacy of the target in standard 51Cr release assays. The signal responsible for accelerated 86Rb+ release has been localized to a postbinding event and has the physiologic requirements of delivery of the lethal hit. Thus, accelerated 86Rb+ release from the CTL represents the first direct observation of a physiologic event within the CTL related to a functional CTL-target interaction.
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002450:Cell Communication; D003601:Cytotoxicity Tests, Immunologic; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D011188:Potassium / Q000378:metabolism / Q000502:physiology; D011868:Radioisotopes; D012413:Rubidium / Q000378:metabolism*; D013602:T-Lymphocytes, Cytotoxic / Q000378:metabolism* / Q000502:physiology; D013696:Temperature; D013997:Time Factors
D011868:Radioisotopes; D012413:Rubidium; D011188:Potassium; D002118:Calcium