[Effect of neurotropic compounds on the transmembrane potential of synaptosomes].
Using the potential-sensitive dye (3,3-dipropylthiodicarbocyanine) [dis-C3-(5)] it has been shown that synaptosomes isolated from the rat brain cortex by the method of Hajôs retain their transmembrane potential (TMP) close to the K+-diffusional one. In the Krebs-Ringer medium containing 5 mM KCl the TMP of the synaptosomes was equal to 70-80 mV. The cytostatics cytochalazine B and colchicine and the peptides leu- and met-enkephalins and tuftcin in concentrations up to 10(-3) and 10(-4) M did not affect the TMP of the synaptosomes. Cytochalazine B in a concentration of 4 X 10(-4) M and over caused synaptosomal aggregation. The neuroleptics aminazine, trifluoroperazine, haloperidol, the antidepressant imipramine in concentrations about 10(-4) M as well as the tranquilizers diazepam and phenazepam in concentrations about 10(-3) M completely depolarized synaptosomal membranes.
D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology; D002746:Chlorpromazine / Q000494:pharmacology; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003975:Diazepam / Q000494:pharmacology; D004743:Enkephalin, Leucine / Q000494:pharmacology; D004744:Enkephalin, Methionine / Q000494:pharmacology; D006220:Haloperidol / Q000494:pharmacology; D007099:Imipramine / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects*; D010455:Peptides / Q000494:pharmacology*; D011619:Psychotropic Drugs / Q000494:pharmacology*; D051381:Rats; D013574:Synaptosomes / Q000187:drug effects*; D014268:Trifluoperazine / Q000494:pharmacology; D014405:Tuftsin / Q000494:pharmacology
D010455:Peptides; D011619:Psychotropic Drugs; D014268:Trifluoperazine; D003571:Cytochalasin B; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; D006220:Haloperidol; D007099:Imipramine; D003975:Diazepam; D014405:Tuftsin; D003078:Colchicine; D002746:Chlorpromazine
Vliianie neĭrotropnykh soedineniĭ na transmembrannyĭ potentsial sinaptosom.