title: Characterization of peanut agglutinin receptors of murine thymocytes.
abstract: Murine thymocytes can be separated on the basis of their agglutinability by peanut agglutinin (PNA) into two broad subpopulations assimilated to immunoincompetent agglutinated PNA+ cells and immunocompetent nonagglutinated PNA- cells. Seven surface membrane components have been isolated by immunoprecipitation using rabbit anti-PNA IgG and Staphylococcus aureus bearing protein A, from PNA-coated radiolabeled immature cells. These components (apparent molecular weights of 180, 175, 130, 115, 65, 26, and 23 kDa) labeled by the galactose oxidase/tritiated sodium borohydride method and by 125I-iodination are glycoproteins which are PNA-receptor sites normally exposed on the surface membrane of PNA+ thymocytes. The nonagglutinated PNA- cells also possess on their surface unmasked receptors for the lectin (175-180 kDa) but in lower amounts. Neuraminidase treatment prior to galactose oxidase/tritiated sodium borohydride labeling shows that the majority of PNA receptors is present on the PNA-thymocyte surface but are masked by sialic acid residues on the terminal position of the oligosaccharidic chains.
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex; D002462:Cell Membrane / Q000276:immunology; D002469:Cell Separation; D002478:Cells, Cultured; D007106:Immune Sera; D008297:Male; D051379:Mice; D011975:Receptors, Mitogen / Q000032:analysis*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D000936:Antigen-Antibody Complex; D007106:Immune Sera; D011975:Receptors, Mitogen; C038223:peanut agglutinin receptor