Heterogeneous pathways of Ca2+ metabolism in the triggering of the proliferative process in rat thymocytes.
Concanavalin A induces in rat thymocytes a calcium uptake at 1 h exposure and proliferative response after 24 h exposure. Phosphodiesterase activity parallels the proliferative response (thymidine uptake). Valinomycin, monensin and a small dose of ouabain also induce calcium uptake, but do not lead to thymidine uptake later. The latter treatments reduce, in some instances drastically, the concanavalin A response with respect to thymidine uptake. Trifluoperazine reduces the unstimulated thymidine uptake and the concanavalin A induced thymidine uptake. These results suggest that calcium has a decisive role in inducing proliferation but that some ways of increasing cellular Ca2+ concentration interfere in other steps with the DNA synthesis.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D008985:Monensin / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000378:metabolism*; D014268:Trifluoperazine / Q000494:pharmacology; D014634:Valinomycin / Q000494:pharmacology
D002147:Calmodulin; D003208:Concanavalin A; D014634:Valinomycin; D014268:Trifluoperazine; D010042:Ouabain; D004247:DNA; D008985:Monensin; D002118:Calcium