Submandibular gland-conditioned medium enhancement of bone marrow colony-forming cells in tumor-bearing murine recipients.
Soft-agar clonogenic assay techniques were used to examine 2 sources of colony-stimulating activity (CSA), submandibular gland-conditioned medium (SMG-CM) and pregnant mouse uterine extract (PMUE), for potentiation of granulocyte-macrophage (GM-CFUc) or monocyte-macrophage (M-CFUc) progenitor cell populations. The femoral populations being examined were aspirated from normal mice and from those bearing 1 of 2 types of tumor: Lewis lung carcinoma (3LL) or thymic lymphoma ascites tumor (EL-4). When PMUE was used as a CSA source, normal animals showed a greater clonogenic response per population than either of the tumor-bearing groups. When SMG-CM was used as a CSA source, the pattern of GM-CFUc response was much different: GM-CFUc magnitudes increased by fourfold to sixfold over normal levels in tumor-bearing animals. M-CFUc response patterns were also significant, being similar in response but smaller.
D000818:Animals; D001854:Bone Marrow Cells; D002277:Carcinoma / Q000503:physiopathology; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D007962:Leukocytes / Q000502:physiology; D008175:Lung Neoplasms / Q000503:physiopathology; D008223:Lymphoma / Q000503:physiopathology; D008264:Macrophages / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000503:physiopathology*; D011247:Pregnancy; D013363:Submandibular Gland / Q000502:physiology*; D013953:Thymus Neoplasms / Q000503:physiopathology; D014599:Uterus / Q000502:physiology
D003115:Colony-Stimulating Factors