Inhibition of normal human granulopoiesis by neuroblastoma cells.
In vitro inhibition of marrow granulopoiesis was produced by a well-characterized human neuroblastoma cell line (CHP 134). A standard double layer, semi-solid agar system was employed in the experiments. The inhibition was present whether the neuroblastoma cells were mixed with the marrow cells or whether they were separated in a contiguous agar layer. Irradiation of the neuroblastoma cells lessened the inhibitory effect but did not eradicate it. Medium conditioned by the neuroblastoma cells had a mild, but not statistically significant, suppressive effect upon granulopoiesis. Additional studies to define the precise mechanism of suppression are underway.
D002455:Cell Division; D002460:Cell Line; D003114:Colony-Forming Units Assay; D003470:Culture Media; D006098:Granulocytes / Q000166:cytology*; D006410:Hematopoiesis; D006801:Humans; D066298:In Vitro Techniques; D009447:Neuroblastoma / Q000473:pathology*
D003470:Culture Media