Adaptation of a protein a binding assay for measurement of immune complexes in sera of rats.
We describe a simple and sensitive method for the detection of circulating immune complexes (CIC) in rats and have applied the method to test sera from tumor-bearing rats. CIC were precipitated preferentially with 6% polyethylene glycol, dissolved, and then bound to Staphylococcus aureus that are Protein A (PA) positive. Rabbit-anti-rat IgG (RARG) antibodies were then added and followed by 125I-PA. The RARG sandwich antibody enhanced the sensitivity of detection of CIC in rats at least 5 fold compared to that observed using 125I-PA without RARG.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats; D013205:Staphylococcal Protein A
D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D013205:Staphylococcal Protein A