title: A detailed serological study of five anti-Jka sera reacting by the antiglobulin technique.
abstract: Detailed serological investigation of five examples of anti-Jka revealed considerable heterogeneity in their characteristics. All the sera were investigated by the antiglobulin technique, with and without complement and by a range of sensitization and testing methods. Three were readily detected in the presence of complement, while two were detectable with anti-IgG, providing a spin test was used. Complement was bound by all and resulted in enhanced reactions, particularly when slide/tile tests were used. The study emphasizes that anti-complement (C3) is an essential component of polyspecific antiglobulin reagents used in cross-matching, enabling the ready detection of these clinically important antibodies.
mesh_terms: D000937:Antigen-Antibody Reactions; D001789:Blood Group Antigens / Q000276:immunology*; D001787:Blood Group Incompatibility / Q000175:diagnosis; D003176:Complement C3 / Q000276:immunology; D003181:Complement C4 / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology / Q000378:metabolism; D003298:Coombs Test; D005260:Female; D006801:Humans; D007518:Isoantibodies / Q000032:analysis*; D007667:Kidd Blood-Group System / Q000276:immunology*; D011247:Pregnancy
chemical_list: D001789:Blood Group Antigens; D003176:Complement C3; D003181:Complement C4; D007518:Isoantibodies; D007667:Kidd Blood-Group System; D003165:Complement System Proteins