Enzyme-linked immunosorbent assay for detection of capsular antibodies against Haemophilus influenzae type b: comparison with radioimmunoassay.
An enzyme-linked immunosorbent assay (ELISA) is described for detection of anti-capsular antibodies against Haemophilus influenzae type b. Polyribosephosphate was covalently bonded to poly-L-lysine before adsorption to microtiter plates. ELISA immunoglobulin G and immunoglobulin M anti-polyribosephosphate antibody titers were comparable to total anti-polyribosephosphate antibody concentration determined by radioimmunoassay. The ELISA technique will be useful for further investigations of host response to infections due to H. influenzae type b but is not intended to be used as a serological method for documenting H. influenzae type b infections.
D000327:Adsorption; D000907:Antibodies, Bacterial / Q000032:analysis*; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D006193:Haemophilus influenzae / Q000276:immunology*; D006801:Humans; D010428:Pentosephosphates / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011863:Radioimmunoassay
D000907:Antibodies, Bacterial; D010428:Pentosephosphates; D011135:Polysaccharides, Bacterial; C003191:polyribose phosphate