[Penicillinase from B. licheniformis. Determination of the ionization constants of the enzymatic hydrolysis products of beta-lactam antibiotics].
The level of transformation of beta-lactam antibiotics hydrolysed by penicillinase from B. licheniformis on determination with the method of pH-metric titration with sodium hydrate solutions depended on the electrochemical nature of the products formed. The data on the study of the pH dependence of the penicillinase-catalysed hydrolysis of beta-lactam antibiotics were used for estimation of the ionization constants of the products of the enzymatic hydrolysis of the beta-lactam ring in the molecules of azlocillin, carfecillin, benzylpenicillin, cephalothin and 7-PADCA. Methods for quantitative determination of the compounds were developed. The methods are based on penicillinase-catalysed enzymatic hydrolysis and pH-metric titration of the products with sodium hydrate solutions at pH 7.0 with regard to their dissociation levels.
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001407:Bacillus / Q000201:enzymology*; D002384:Catalysis; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007477:Ions; D010405:Penicillinase / Q000378:metabolism*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D007477:Ions; D047090:beta-Lactams; D010405:Penicillinase
Penitsillinaza iz 8. licheniformis. Opredelenie konstant ionizatsii produktiv fermentativnogo gidroliza betalaktamnykh antibiotikov.