A radiobinding assay for human H-Y antigen using monoclonal antibodies.
Radiobinding assays using monoclonal H-Y antibodies were performed on human peripheral blood leukocytes, concanavalin-A-(conA)3 stimulated blast cells, and fibroblast primary cell cultures. For peripheral blood leukocytes, male-specific binding was demonstrated only when the cells were enriched for T cells and the H-Y antisera were partially purified for the IgG or IgM fractions. Similar results were obtained with con-A-stimulated blast cells. Fibroblast cells could be tested with unpurified H-Y antisera. With our monoclonal antibodies, best results were obtained using con A blast cells and fibroblasts.
D000911:Antibodies, Monoclonal; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts / Q000276:immunology; D006182:H-Y Antigen / Q000032:analysis*; D006801:Humans; D007962:Leukocytes / Q000276:immunology; D008297:Male; D011863:Radioimmunoassay; D012737:Sex Factors; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D006182:H-Y Antigen; D003208:Concanavalin A