title: Comparison of media for isolation of poultry intestinal bacteria.
abstract: The effects of medium composition, incubation temperature, and length of incubation were determined for recovery of the predominant intestinal bacteria from turkey poults. Incubation of recovery media at 41 degrees C resulted in significantly higher counts than at 37 degrees C. In 2- and 3-week-old turkey poults. RGCAP-30, RGCAP-10, and RGCA-30 gave the highest recoveries of cecal bacteria. M98-5 was less effective and brain heart infusion agar was definitely inadequate. However, there was no significant difference between RGCAP-30 and brain heart infusion agar for recovery of duodenal bacteria. In older birds (6 weeks of age), M98-5 was equal or superior to the RGCA-based media. The choice of a primary isolation medium is thus dependent on the site to be sampled and the age of the bird.
mesh_terms: D000375:Aging; D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D002432:Cecum / Q000382:microbiology; D003470:Culture Media; D007421:Intestine, Small / Q000382:microbiology; D007422:Intestines / Q000382:microbiology*; D013696:Temperature; D014422:Turkeys / Q000382:microbiology*
chemical_list: D003470:Culture Media