Age-associated changes of the characteristics of autoantibody-forming cells in NZB/W mice.
Age-associated change of characteristics of anti-double-stranded (ds) DNA antibody-forming cells in the NZB/W mouse spleen was studied. These cells in mice aged 9-11 months, which develop high titer anti-ds DNA antibody formation, produced antibody without the help of T cells or pokeweed mitogen (PWM), adhered to Sephadex G-10, and sedimented to the bottom when applied to Ficoll-Paque solution. On the other hand, these cells in relatively young (7- to 8-month-old) mice, which develop low titer anti-ds DNA antibody formation, produced antibody maximally with the help of T cells and PWM, adhered little to Sephadex G-10, and fractionated into intermediate lymphocyte fractions when applied to Ficoll-Paque solution. Collectively, anti-ds DNA antibody-forming cells in NZB/W mice were shown to change their characteristics with age.
D000375:Aging; D000818:Animals; D000974:Antibodies, Antinuclear / Q000096:biosynthesis; D000921:Antibody-Producing Cells / Q000166:cytology; D004247:DNA / Q000276:immunology; D005260:Female; D051379:Mice; D008814:Mice, Inbred NZB; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013046:Specific Gravity; D013601:T-Lymphocytes / Q000502:physiology
D000974:Antibodies, Antinuclear; D011043:Pokeweed Mitogens; D004247:DNA