Characterization of a scyllo-inositol-containing sialyloligosaccharide from normal human urine.
Three inositol-containing sialyloligosaccharides were isolated from normal human urine. Structural studies including gas-liquid chromatography of mono- and disaccharide derivatives, methylation analysis, mass spectrometry and glycosidase treatments indicated the structure NeuAc (alpha 2-3)Gal(beta 1-0)scyllo-inositol for one of the oligosaccharides isolated. This provides the first evidence for the natural occurrence of a scyllo-inositol glycoside in biological material. The two other oligosaccharides isolated were identified as two isomers of NeuAc(alpha 2-3)Gal(beta 1-0)myo-inositol, which have not been identified in normal urine before.
D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007294:Inositol / Q000032:analysis*; D009844:Oligosaccharides / Q000652:urine*; D012016:Reference Values
D009844:Oligosaccharides; C016735:sialooligosaccharides; C009217:scyllitol; D007294:Inositol