Determination of bethanidine in plasma by liquid-chromatography with a microbore reversed-phase column.
In this novel method for quantifying bethanidine in plasma, after a multi-step extraction of bethanidine and internal standard from 2.0 mL of plasma, the drugs are separated on a "microbore" C18 reversed-phase column and quantified by their ultraviolet absorbance at 210 nm. The isocratic chromatographic separation takes about 15 min with use of an ion-pairing regent in the mobile phase (acetate buffer/acetonitrile, 9/1 by vol) and a flow rate of 0.25 mL/min. Sensitivity is increased relative to conventional columns, and solvent consumption is reduced by 90%. The standard curve is linear to at least 5 mg/L, and the detection limit is 0.02 mg/L. The within-run precision of the method is excellent (CV 4%) at a midrange concentration of 1.25 mg/L.
D001627:Bethanidine / Q000008:administration & dosage / Q000097:blood / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods; D004334:Drug Administration Schedule; D006146:Guanidines / Q000302:isolation & purification*; D006207:Half-Life; D006801:Humans; D011530:Protriptyline / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet
D006146:Guanidines; D011530:Protriptyline; D001627:Bethanidine