Development of a radioimmunoassay for rat liver metallothionein-I and its application to the analysis of rat plasma and kidneys.
A sensitive radioimmunoassay for rat liver metallothionein-I has been developed using avid and high-titre antibodies obtained from sheep that were immunized with a conjugate of metallothionein and rabbit immunoglobulin G. The assay was specific for metallothionein-I, and did not depend on the particular metal bound to the protein. There was no significant cross-reaction with rat liver metallothionein-II. The use of the assay to measure metallothionein concentrations in rat plasma and kidneys is described.
D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D003300:Copper / Q000378:metabolism / Q000494:pharmacology; D003429:Cross Reactions; D005296:Ferrous Compounds / Q000494:pharmacology; D007668:Kidney / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D008667:Metalloproteins / Q000032:analysis*; D008668:Metallothionein / Q000032:analysis* / Q000097:blood; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D015032:Zinc / Q000494:pharmacology
D005296:Ferrous Compounds; D008667:Metalloproteins; D002104:Cadmium; D003300:Copper; D008668:Metallothionein; D015032:Zinc