Estimation of the equilibrium constant for an antibody using data derived from an antiserum dilution curve.
An equation is derived from the Law of Mass Action which enables the equilibrium constant of an antibody to be calculated directly from data obtained from an antiserum dilution curve. A graphical solution is obtained and examples using theoretically derived and experimental data are given.
D000937:Antigen-Antibody Reactions; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006854:Hydrocortisone / Q000032:analysis; D011863:Radioimmunoassay
D006854:Hydrocortisone