Identification and in situ localization of the "thymic nurse cell" in man.
The observation of the "thymic nurse cell" (TNC), a reticuloepithelial cell with intracytoplasmic lymphocytes, in suspension of murine thymic tissue prompted us to investigate the existence of this cell in cell suspension, as well as in tissue sections of the human thymus. TNC-like cells were enriched in suspension by enzymatic disintegration of thymic tissue and 1 X G sedimentation over 50% fetal calf serum gradients. TNC-like cells were negative for lysosomal enzymes: in this respect, as well as in light microscopic morphology, the cells were different from tissue macrophages with intracytoplasmic lymphocytes. In electron microscopy, TNC-like cells showed reticuloepithelial characteristics. In 1-micron tissue sections, clusters of lymphocytes with a possible reticuloepithelial nucleus were observed close to blood capillaries in the cortical area. Ultrastructural analysis confirmed the epithelial nature of this cell, as well as its location adjacent to blood capillaries. We concluded that there is in situ existence of TNC in man. This observation enables studies on the role of TNC in intrathymic T cell maturation.
D002454:Cell Differentiation; D002648:Child; D002675:Child, Preschool; D004848:Epithelium / Q000648:ultrastructure; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007223:Infant; D013601:T-Lymphocytes / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure*; D013950:Thymus Gland / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure*
D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II