title: In vitro neurotoxic esterase assay using leptophos oxon analogs as inhibitors.
abstract: In comparing neurotoxic esterase (NTE) inhibition properties of a series of phenylphosphonates, it was discovered that certain compounds including leptophos inhibited mipafox-insensitive phenylvalerate hydrolases. This leads to erroneous values for NTE inhibition which can be corrected by a differential assay: the total amount of mipafox-insensitive activity is determined with O-(2,6-dichlorophenyl)O-methyl phenylphosphonate and subtracted from the apparent NTE determined with the test compound before calculating pI50's.
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors*; D002645:Chickens; D007306:Insecticides / Q000633:toxicity*; D010773:Leptophos / Q000031:analogs & derivatives*; D009943:Organophosphorus Compounds / Q000633:toxicity; D013329:Structure-Activity Relationship
chemical_list: D007306:Insecticides; D009943:Organophosphorus Compounds; C008265:leptophos oxon; D010773:Leptophos; D002265:Carboxylic Ester Hydrolases; C021859:neurotoxic esterase