[Radioautographic study of nonhistone protein synthesis in the process of lens and retina regeneration in adult tritons].
The incorporation of 3H-tryptophane (3H-T) in the cell nuclei of pigment epithelium, retinal rudiment, iris and vitreous body has been studied during lens and retina regeneration in the adult smooth newts. A gradual increase (7 to 13-fold as compared with the control) of the intensity of labelling was found in the cell nuclei of pigment epithelium of the central part of fundus oculi. The cells of this zone are a source of regeneration. In the peripheral zones which do not participate in retina regeneration the intensity of 3H-T incorporation increased 2 to 4-fold only. The area of cell nuclei of pigment epithelium of the central part of fundus oculi increased, whereas that in the peripheral part suffered no changes. The cell nuclei of iris, retinal and lens rudiments incorporated 3H-T intensively. In the iris the most intensive labelling was shown in the inner layer of its dorsal zone which is a source of lens regeneration. The incorporation of 3H-T in the vitreous body was also found, thus suggesting both the secretion of vitreous body by the regenerating retina cells and the migration of some proteins secreted by the regenerating retina from its surface in the direction of a lens regenerating from the dorsal iris.
D000818:Animals; D001345:Autoradiography; D002467:Cell Nucleus / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis*; D005260:Female; D007908:Lens, Crystalline / Q000502:physiology*; D008297:Male; D012038:Regeneration; D012160:Retina / Q000502:physiology*; D013997:Time Factors; D014316:Tritium; D014319:Triturus / Q000502:physiology*; D014364:Tryptophan / Q000378:metabolism
D002868:Chromosomal Proteins, Non-Histone; D014316:Tritium; D014364:Tryptophan
Radioavtograficheskoe issledovanie sinteza negistonovykh belkov v protsesse regeneratsii khrustalika i setchatki u vzroslykh tritonov.