A diffusible molecule may mediate glutamate neurotoxicity.
Purified neuronal cultures from 8-day chick embryo neural retinas were incubated in either serum-free N1 or N1 supplemented with heart extract. The addition of glutamate decreased neuronal survival only in the presence of heart extract; however, if heart extract was dialyzed, neuronal survival remained at control values. This suggests a diffusible molecule may mediate glutamate neurotoxicity Kainic acid decreased neuronal survival in all conditions tested.
D000818:Animals; D001696:Biomechanical Phenomena; D002470:Cell Survival; D002478:Cells, Cultured; D002642:Chick Embryo; D005971:Glutamates / Q000633:toxicity*; D007608:Kainic Acid / Q000633:toxicity; D009474:Neurons / Q000187:drug effects*; D012160:Retina / Q000166:cytology
D005971:Glutamates; D007608:Kainic Acid