Unbalanced X chromosome mosaicism in B cells of mice with X-linked immunodeficiency.
The immunodeficiency in CBA/N mice is reflected by abnormal development of a subset of B lymphocytes. However, it is not clear how xid, the mutant gene in CBA/N mice, affects the development of this subset. Specifically, it is not known if the xid gene influences the development of the B cell subset directly or indirectly by providing the improper developmental milieu through effects on other cells. We investigated this question using female mice heterozygous for two x chromosomal genes, xid and Pgk-1 (phosphoglycerate kinase-1). Since females are mosaic because of x chromosome inactivation, their lymphocytes can be studied for the choice of the x chromosome, using the two PGK-1 isoenzymes as the cytological marker. We find that B lymphocytes in the spleen prefer the x chromosome without xid while the remaining splenocytes and cells from other tissues do not. This suggests that xid affects B lymphocytes directly and not through their developmental milieu. Furthermore, our data suggest that the precursors for IgG1- and IgG3-producing cells may be both few and different.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001921:Brain / Q000201:enzymology; D005260:Female; D008040:Genetic Linkage; D006412:Hematopoietic Stem Cells / Q000201:enzymology; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000276:immunology*; D007527:Isoenzymes / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D051379:Mice; D008808:Mice, Inbred CBA / Q000235:genetics*; D009030:Mosaicism; D010735:Phosphoglycerate Kinase / Q000378:metabolism; D012729:Sex Chromosome Aberrations / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000166:cytology; D014960:X Chromosome / Q000201:enzymology
D007074:Immunoglobulin G; D007527:Isoenzymes; D010735:Phosphoglycerate Kinase