Cross-reactive idiotypes on heterologous anti-allotype antibody.
Specific anti-a1 Ab was isolated from rabbits, guinea pigs, mice, chickens, and a goat. Each of these preparations was able to inhibit the reaction between a1 IgG and rabbit anti-a1 Ab as well as the reaction between rabbit IdX (anti-a1 Ab) and rabbit anti-IdX (anti-anti-a1 Ab). Maximal levels of inhibition ranged from 75 to 100% in the latter assay. Although the relative binding efficiencies of each preparation varied widely, there was generally a positive correlation between the ability of an anti-allotype reagent to bind to a1 IgG and to anti-IdX Ab. Each of the heterologous anti-a1 Ab samples was able to form precipitin bands with rabbit anti-Id Ab. These bands fused with each other and with rabbit anti-a1 Ab. These results weaken the interpretation that the ubiquitous expression of IdX previously observed in the rabbit reflects shared conserved genes. We suggest that either 1) the anti-Id Ab represents high fidelity internal images of the a1 epitopes, or 2) latent a1 allotype Ig is present in the anti-IdX preparation. In either case, any anti-a1 Ab would be potentially reactive with the anti-IdX preparation.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D000918:Antibody Specificity; D001667:Binding, Competitive; D002645:Chickens; D003429:Cross Reactions; D006041:Goats; D006168:Guinea Pigs; D007126:Immunoglobulin Allotypes / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes