A simple quantitative method for the determination of 3-fluorotyrosine substitution in proteins.
A rapid quantitative method is described for determining 3-fluorotyrosine incorporation into proteins. Derivatives of tyrosine and 3-fluorotyrosine with o-phthalaldehyde are well separated from one another by a reverse-phase high-performance liquid chromatography system used for routine analyses of o-phthalaldehyde-amino acid derivatives. Since both amino acids are well resolved from all other derivatized amino acids, the method is useful for amino acid analyses of proteins. Determination of the fluorotyrosine content of proteins by this method involves a single separation step, is reproducible, and requires no corrections for stability or yield. Further, the o-phthalaldehyde derivatives of 5-fluorotryptophan, 2-fluorophenylalanine, 3-fluorophenylalanine, and 4-fluorophenylalanine can also be resolved. The method may be generally applicable to fluorinated aromatic amino acid-labeled proteins that are studied structurally and dynamically by nuclear magnetic resonance.
D002851:Chromatography, High Pressure Liquid; D003090:Coliphages / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D009764:o-Phthalaldehyde
D011506:Proteins; C001245:3-fluorotyrosine; D014443:Tyrosine; D009764:o-Phthalaldehyde