title: Phospholipase C activity in plasma membranes isolated from lapine synovial cells in monolayer culture.
abstract: Plasma membranes were isolated from lapine synovial cells grown in monolayer culture using discontinuous sucrose gradient centrifugation techniques. 5'nucleotidase was detected in great abundance while glucose-6 phosphate dehydrogenase and cytochrome oxidase were present at low to undetectable levels. Plasma membranes incubated at 37 degrees C for 60 min with [3H]-arachidonyl-phosphatidylinositol/phosphatidylserine synthesized [3H]-diacylglycerides. Little if any [3H]-diacylglyceride synthesis was measured when [3H]-arachidonyl-phosphatidylcholine or [3H]-arachidonyl-phosphatidylethanolamine were used as substrates. These results are consistent with a plasma membrane-associated phosphatidylinositol-specific phospholipase C from lapine synovial cells in culture.
mesh_terms: D000818:Animals; D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D005260:Female; D007700:Kinetics; D010740:Phospholipases / Q000378:metabolism*; D011817:Rabbits; D013583:Synovial Membrane / Q000201:enzymology*; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: D010740:Phospholipases; D010738:Type C Phospholipases