Flow-cytometric monitoring of intracellular flavins simultaneously with NAD(P)H levels.
A cytofluorimeter is described, using the combination of Argon UV (351-363 nm) and Argon Blue (488 nm) lasers. The dual excitation makes it possible to monitor simultaneously the redox state of flavins and NAD(P)H as indicative of cell metabolic state. Light scatter, absorption, and staining with exogenous fluorescent dyes can add additional information. Thus, a five-parameter flow analysis becomes possible. The present paper describes flavin and NAD(P)H measurements on isolated rat liver cells and mouse bone marrow.
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D005415:Flavins / Q000378:metabolism*; D005434:Flow Cytometry; D005453:Fluorescence; D008099:Liver / Q000378:metabolism; D051379:Mice; D009243:NAD / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction; D011190:Potassium Cyanide / Q000494:pharmacology; D051381:Rats
D005415:Flavins; D009243:NAD; D009249:NADP; D011190:Potassium Cyanide