Production of a monoclonal antibody to autoantigenic components of human glomerular basement membrane.
We describe a mouse monoclonal antibody which reacts on immunoblotting with those components of collagenase digested human glomerular basement membrane (GBM) that are also recognized by autoantibodies in sera from patients with anti-GBM nephritis. Competition between the monoclonal antibody and anti-GBM autoantibodies was demonstrated in a solid phase radioimmunoassay, suggesting that both are directed against the same autoantigen.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000941:Antigens / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D001485:Basement Membrane / Q000276:immunology; D006801:Humans; D007678:Kidney Glomerulus / Q000276:immunology* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000941:Antigens; D001324:Autoantigens