Cytolethal sensitivity of human lymphoid cells to glucocorticoids and oxidised polyamines.
A series of clonally-derived glucocorticoid-sensitive and -resistant human lymphoid cell lines was used to investigate the relationship between sensitivity to the effects of oxidised polyamines and initiation of glucocorticoid-induced cytostatic and cytolethal responses. Whilst the exogenous polyamines were found to exert no effect by themselves, incubation of cells for 48 h with 10(-4)M spermine or spermidine in the presence of serum polyamine oxidase produced severe lethal responses in all clones tested. By contrast 10(-5)M exogenous polyamines in the presence of polyamine oxidase produced lethal effects only in glucocorticoid-sensitive clones. Spermine was more potent than spermidine. The significance of these observations is discussed.
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology; D004351:Drug Resistance; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D011073:Polyamines / Q000494:pharmacology*; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology
D005938:Glucocorticoids; D011073:Polyamines; D013096:Spermine; D003907:Dexamethasone; D013095:Spermidine