Peritoneal exudate in larval Rana pipiens contains cells that are embryologically derived from dorsal lateral plate mesoderm.
The embryonic origin of cells functioning in a peritoneal exudate response of larval Rana pipiens was investigated. Normal larva received injections of sodium caseinate on day 0 and cells in the peritoneal cavity were assayed on days 3, 5, 7, 9, 11, 13 and 16. Experimental animals that received cytogenetically labeled transplants of dorsal mesoderm at 67-72 hours of development were injected with sodium caseinate on day 0 and assayed on days 5, 7 and 9. Feulgen-DNA microdensitometric analysis showed that the peritoneal exudate from days 5, 7, 9 contained hemopoietic cells that were derived from the embryonic transplant. Therefore, dorsal mesoderm gave rise to cells capable of functioning in a peritoneal exudate response to injections of sodium caseinate.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002364:Caseins / Q000276:immunology; D002454:Cell Differentiation; D005122:Exudates and Transudates / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology; D007814:Larva; D008648:Mesoderm; D010586:Phagocytes / Q000166:cytology; D011894:Rana pipiens / Q000196:embryology* / Q000276:immunology
D002364:Caseins