Description of a mouse monoclonal anti-HLA-B27 antibody HLA-ABC-m3.
The production and characterization of a new anti-HLA-B27 monoclonal antibody HLA-ABC-m3 is described. This cytotoxic IgG2a antibody binds protein A and is able to precipitate cell surface molecules of 43,000 and 12,000 daltons corresponding to the HLA heavy chain and beta 2-microglobulin. Population testing revealed that the HLA-ABC-m3 antibody reacted with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27+ and with 5/105 HLA-B27- individuals. These five extra reactions were with individuals expressing the cross-reactive HLA-B7 alloantigen, although the affinity of the monoclonal antibody for B27 heterozygous individuals (approx 10(9) M-1) was tenfold greater than with B7 individuals (approx 10(8) M-1). In addition, HLA-ABC-m3 reactivity segregated with HLA-B27 in two families. This monoclonal antibody should be of value in the investigation of the role of HLA-B27 in disease.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D003429:Cross Reactions; D006680:HLA Antigens / Q000276:immunology*; D015796:HLA-B27 Antigen; D015793:HLA-B7 Antigen; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007120:Immunochemistry; D007152:Immunologic Capping; D051379:Mice
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D015796:HLA-B27 Antigen; D015793:HLA-B7 Antigen