Inhibition of anion transport across the red cell membrane by dinitrophenylation of a specific lysine residue at the H2DIDS binding site of the band 3 protein.
The inhibition of anion transport by dinitrophenylation of the red cell membrane is brought about by the modification of a single lysine residue located on the 17-kDa segment of the band 3 protein. This residue is identical with Lys a, which is also capable of reacting with one of the two isothiocyanate groups of 4,4'-diisothiocyano dihydro-stilbene-2,2'-disulfonate (H2DIDS). The rate of reaction between Lys a and 1-fluoro-2,4-dinitrobenzene is reduced when a second lysine residue on the 35-kDa segment of the band 3 protein becomes dinitrophenylated. This latter residue is not identical with Lys b which is known to be present on the 35-kDa segment and involved in the cross-linking of this segment with the 17-kDa segment by H2DIDS.
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000031:analogs & derivatives*; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D000345:Affinity Labels / Q000494:pharmacology*; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D027321:Anion Transport Proteins; D000838:Anions; D001692:Biological Transport / Q000187:drug effects; D002250:Carbon Radioisotopes; D002352:Carrier Proteins / Q000378:metabolism*; D004139:Dinitrofluorobenzene / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009578:Nitrobenzenes / Q000494:pharmacology*; D013267:Stilbenes / Q000494:pharmacology*; D013431:Sulfates / Q000097:blood; D014316:Tritium
D000345:Affinity Labels; D001457:Anion Exchange Protein 1, Erythrocyte; D027321:Anion Transport Proteins; D000838:Anions; D002250:Carbon Radioisotopes; D002352:Carrier Proteins; D009578:Nitrobenzenes; D013267:Stilbenes; D013431:Sulfates; D014316:Tritium; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; C018564:dihydro-DIDS; D004139:Dinitrofluorobenzene; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid