Lymphokine-induced IgM secretion by clones of neoplastic B cells.
The induction of antibody secretion by B cells requires T-cell-derived factors1-5. Such factors have been described1,2,6-12 but the precise relationship among these various factors is not clear, and it has been difficult to demonstrate that these factors act directly on the B cell and do not exert their effect via T cells or macrophages. In this report we describe the direct induction of IgM synthesis and secretion in cloned lines of long-term tissue culture adapted neoplastic B cells (BCL1) by T-cell supernatants from phorbol-12-myristate 13-acetate (PMA)-induced EL-4 cells or concanavalin A (Con A)-induced 7.1.1a cells5,9. We have termed this activity BCDFmu (B-cell differentiation factor for IgM). The supernatants containing BCDFmu induce activated and neoplastic B cells to secrete IgM5 and the factor responsible is distinct from BCGF13, interleukin-2 (IL-2)5, the classical T-cell replacing factor (TRF) described by Schimpl and Wecker5, and immune interferon (IFN gamma)5.
D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002460:Cell Line; D002999:Clone Cells; D007075:Immunoglobulin M / Q000235:genetics*; D007700:Kinetics; D008222:Lymphokines / Q000494:pharmacology*; D008223:Lymphoma / Q000473:pathology; D008970:Molecular Weight; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification
D007075:Immunoglobulin M; D008222:Lymphokines; D012333:RNA, Messenger