Biological consequences of neuraminidase deficiency in Newcastle disease virus.
A second-step revertant (L1) of a temperature-sensitive mutant (C1) of Newcastle disease virus agglutinated erythrocytes normally but had less than 3% of the wild-type (strain AV) levels of neuraminidase activity. Revertant L1 had seven times more virion-associated N-acetylneuraminic acid (NANA) than strain AV. NANA residues on purified virions were specifically labeled with periodate and tritiated borohydride. Analyses of radiolabeled L1 virions on sodium dodecyl sulfate-polyacrylamide gels showed that most of the virion-associated NANA was in a high-molecular-weight component with an electrophoretic mobility different from that of any known viral protein. NANA was also detected in molecules with the electrophoretic mobility of the viral glycoproteins HN and F1. Revertant L1 had a twofold lower rate constant of attachment to HeLa cells than that of the wild-type. Treatment of L1 virions with Vibrio cholerae neuraminidase removed the excess NANA and returned L1 attachment kinetics to normal. Revertant N1, which has 10-fold more neuraminidase activity than L1, penetrated host cells at the same rate as L1. L1 was impaired in elution from erythrocytes. Removal of virion-associated NANA exacerbated this defect. Despite a small disadvantage in attachment and a major defect in elution relative to strain AV, revertant L1 enjoyed a slight advantage over the wild-type during a single reproductive cycle in cultured chicken embryo cells.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004912:Erythrocytes / Q000382:microbiology; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D009439:Neuraminidase / Q000378:metabolism*; D009522:Newcastle disease virus / Q000032:analysis / Q000201:enzymology* / Q000502:physiology; D012794:Sialic Acids / Q000032:analysis / Q000502:physiology; D014779:Virus Replication
D012794:Sialic Acids; D009439:Neuraminidase