A comparison of fluorescamine and o-phthaldialdehyde as effective blocking reagents in protein sequence analyses by the Beckman sequencer.
Use of o-phthaldialdehyde to chemically reduce the newly generated amino termini responsible for the progressively increasing background during an extended amino acid sequence analysis in a liquid phase sequencer has been described. The results have been compared with Fluram blocking using apomyoglobin and rabbit C-reactive protein as standard and unknown samples, respectively.
D000447:Aldehydes; D000595:Amino Acid Sequence; D000818:Animals; D001059:Apoproteins; D002097:C-Reactive Protein / Q000032:analysis*; D005450:Fluorescamine; D009211:Myoglobin / Q000032:analysis*; D010084:Oxidation-Reduction; D011817:Rabbits; D013141:Spiro Compounds; D014907:Whales; D009764:o-Phthalaldehyde
D000447:Aldehydes; D001059:Apoproteins; D009211:Myoglobin; D013141:Spiro Compounds; C010857:apomyoglobin; D005450:Fluorescamine; D009764:o-Phthalaldehyde; D002097:C-Reactive Protein