Inhibition of interleukin synthesis and T cell proliferation by a monoclonal anti-Ia antibody.
The proliferative response in vitro of Lewis rat spleen cells to Con A was inhibited by a monoclonal antibody, OX4, with specificity for a public rat la antigen. Inhibition was dose-dependent and was observed only if the antibody was present during the first 48 hr of culture. OX4 anti-la antibody inhibited production of IL 1 by peritoneal exudate cells activated by lipopolysaccharide and inhibited the production of IL 2 by spleen cells activated by Con A. Addition of exogenous IL 1 to spleen cell cultures inhibited by OX4 anti-la antibody augmented production of IL 2 and returned the proliferative response of T cells to levels observed in Con A-stimulated cultures. We suggest that OX4 anti-la antibody reacts with la+ accessory cells to inhibit production of IL 1, which in turn limits production of IL 2 and finally T cell proliferation.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D001202:Ascitic Fluid / Q000166:cytology; D003208:Concanavalin A / Q000494:pharmacology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007108:Immune Tolerance; D007375:Interleukin-1 / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D007375:Interleukin-1; D007376:Interleukin-2; D008070:Lipopolysaccharides; D003208:Concanavalin A