[Immunogenic, tissue-specific, antigenic determinants of thymocyte chromatin--firmly bound to nonhistone proteins].
Rat thymocyte chromatin was dissolved in 1.0 or 2.0 M NaCl and the dissociated proteins were separated by ultracentrifugation or gel filtration on Sephadex G-200. Rabbit antibodies against tissue-specific antigenic determinants of thymus chromatin interact with "depleted" deoxynucleoproteins and not with dissociating proteins. The DNA complex with firmly bound proteins obtained in 2 M NaCl was treated with DNAase I; this treatment had no effect on proteins interaction with antibodies. Thus, tissue-specific antigenic determinants can be related to the most firmly bound nonhistone proteins.
D000818:Animals; D002843:Chromatin / Q000276:immunology*; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology*; D004247:DNA / Q000276:immunology*; D008297:Male; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004247:DNA
Immunogennye tkanespetsificheskie antigennye determinanty khromatina timotsitov--prochno sviazannye negistonovye belki.