Radioimmunoassay of the immunomodulator erythro-9-(2-hydroxy-3-nonyl)-hypoxanthine in human serum and urine.
A radioimmunoassay was developed for the measurement in human serum and urine of erythro-9-(2-hydroxy-3-nonyl)-hypoxanthine. Antisera were produced in rabbits by immunization with an erythro-9-(2-hydroxy-3-nonyl)-hypoxanthine hemisuccinate-bovine serum albumin conjugate. The competitive antigen was erythro-9-(2-hydroxy-3-nonyl)-hypoxanthine labeled with carbon-14 on the purine ring. Cross-reactivities were measured against three metabolites and the naturally occurring purine bases inosine and hypoxanthine. Sensitivity of the method was 1 ng/ml in serum and 10 ng/ml in urine. Precision at clinical levels was +/- 15% in serum at 2 ng/ml and +/- 3% in urine at 200 ng/ml.
D000276:Adjuvants, Immunologic / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D007042:Hypoxanthines / Q000032:analysis* / Q000097:blood / Q000652:urine; D011863:Radioimmunoassay / Q000379:methods
D000276:Adjuvants, Immunologic; D007042:Hypoxanthines; C026533:NPT 15392