The spatial arrangement of the complex between eukaryotic initiation factor eIF-3 and 40 S ribosomal subunit. Cross-linking between factor and ribosomal proteins.
The binding site for eIF-3 on the small ribosomal subunit was studied (a) by use of a complex of eIF-3 and derived 40 S ribosomal subunit from rat liver, and (b) by use of native small ribosomal subunits from rabbit reticulocytes. After treatment of both complexes with dimethyl 4,7-dioxo-5,6-dihydroxy-3,8-diazadecanbisimidate ribosomal proteins S3a, S4, S6, S7, S8, S9, S10, S23/24 and S27 became covalently linked to eIF-3 and were isolated together with the factor by gradient centrifugation. The ribosomal proteins were identified by two-dimensional polyacrylamide gel electrophoresis after periodate cleavage of the link(s).
D000818:Animals; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D039621:Eukaryotic Initiation Factor-3; D007096:Imidoesters / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism*; D010448:Peptide Initiation Factors / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D011817:Rabbits; D051381:Rats; D012156:Reticulocytes / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
D003432:Cross-Linking Reagents; D039621:Eukaryotic Initiation Factor-3; D007096:Imidoesters; D010448:Peptide Initiation Factors; D012269:Ribosomal Proteins; C030506:dimethyl-3,8-diaza-4,7-dioxo-5,6-dihydroxydecanbis(imidate)