Tetanus toxin blocks Ca spikes in neuroblastoma clone N1E-115 cells.
The effect of tetanus toxin on the Ca spike of the action potential evoked in neuroblastoma clone N1E-115 cells by constant current stimulation in Na+-free medium containing various concentrations of Ca2+(1.8-80 mM) was examined by intracellular recordings. Tetanus toxin (more than 140 ng/ml) blocked the Ca spikes. The resting membrane potentials and membrane input resistances of tetanus toxin-treated cells were similar to those of untreated cells. The blocking effect of tetanus toxin was evident in medium containing Ca2+ at concentrations of more than 30 mM. The difference between the peak levels of the action potentials of toxin-treated and untreated cells was statistically significant at Ca2+ concentrations of 7.2 mM and more.
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002999:Clone Cells; D007700:Kinetics; D008564:Membrane Potentials; D051379:Mice; D009447:Neuroblastoma / Q000503:physiopathology*; D013744:Tetanus Toxin / Q000494:pharmacology*
D013744:Tetanus Toxin; D002118:Calcium