title: The phosphorylase kinase activity of hearts from phosphorylase kinase-deficient mice.
abstract: In an assay measuring radioactive incorporation from gamma--P32P]ATP into phosphorylase b, cardiac muscle extracts from mice with the phosphorylase kinase deficiency mutation showed significant, calcium-dependent phosphorylase kinase activity that was 10 to 15% of that of Swiss mice, the control strain. Isoproterenol stimulated significant phosphorylase a accumulation in both isolated atria and right ventricular strips of phosphorylase kinase-deficient mice, and the drug-stimulated increases in phosphorylase a activity the the contractile responses of right ventricular strips were similar in Swiss and phosphorylase kinase/deficient mice.
mesh_terms: D000818:Animals; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D051379:Mice; D009154:Mutation; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000201:enzymology*; D010764:Phosphorylase Kinase / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D010762:Phosphorylase a / Q000096:biosynthesis
chemical_list: D010762:Phosphorylase a; D010764:Phosphorylase Kinase; D007545:Isoproterenol