Fate of DNA in eclipse complex during genetic transformation in Streptococcus pneumoniae.
Uptake of DNA and genetic recombination proceeded normally in competent Streptococcus pneumoniae despite inhibition of DNA replication by 6-(p-hydroxyphenylazo)-uracil. Immediately after a brief uptake period, 68% of donor DNA label was in eclipse complex form, and 22% was in low-molecular-weight products; by the completion of integration at 10 min, 23% was integrated into the chromosome, and the rest was lost from the cell. Throughout the process, less than 1% was found as free single strands. The DNA in eclipse complex is therefore an intermediate in the integration process.
D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D006906:Hydroxyphenylazouracil / Q000494:pharmacology; D007700:Kinetics; D011995:Recombination, Genetic / Q000187:drug effects; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000235:genetics*
D004269:DNA, Bacterial; D006906:Hydroxyphenylazouracil