title: In vitro inhibition of rat oocyte meiosis by human follicular fluid fractions.
abstract: We studied the effect of low molecular weight fractions of human follicular fluid (FF1) on rat oocyte meiosis and progesterone secretion by the granulosa cells. Steroid-free FF1 extracts were filtered through Sephadex G50 gel. One highly retarded fraction was obtained (GF-3), which was either used for testing or further purified by filtration on Sephadex G10, the G10-3 fraction of which was used for experiments. The GF-3 and G10-3 fractions of FF1 inhibited (in a dose-dependent and reversible manner) meiosis of isolated rat oocytes during a 4-h culture. Similarly treated fractions of serum had no effect. The inhibition was not abolished by ether extraction, trypsin treatment, heating to 56 degrees C for 1 h or boiling for 5 min, whereas heating to 105 degrees C for 18 h decreased the effect. Gonadotropin-stimulated progesterone secretion by cumulus and mural granulosa cells was also dose-dependently and reversibly inhibited by the GF-3 fractions. Our results confirm those obtained in earlier studies on porcine oocyte maturation inhibitor (OMI).
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002850:Chromatography, Gel; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008540:Meiosis / Q000187:drug effects; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development*; D006080:Ovarian Follicle / Q000502:physiology*; D010455:Peptides / Q000494:pharmacology*; D011374:Progesterone / Q000378:metabolism; D051381:Rats; D014020:Tissue Extracts / Q000494:pharmacology
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D014020:Tissue Extracts; C018355:intraovarian peptides; D011374:Progesterone