Kinetics of the antigen:antibody reaction. Light scattering and particle size measurement using photon correlation spectroscopy.
Many techniques for the estimation of specific proteins measure light scattered by, or the turbidity of, suspensions of antigen:antibody aggregates formed in fluid-phase reactions. Although the shapes of reaction curves obtained are well documented, interpretation of the data in relation to the laws of light scattering and the mechanism of the antigen:antibody reaction are poorly described in the literature. Using photon correlation spectroscopy it has been shown that antigen:antibody complexes continue to grow in size even when no further increases in scatter intensity or turbidity are apparent.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis*; D006801:Humans; D007106:Immune Sera; D007074:Immunoglobulin G; D007700:Kinetics; D012756:Sheep / Q000276:immunology; D013053:Spectrophotometry / Q000379:methods
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007074:Immunoglobulin G