Manipulation of anti-LDH-B response by T suppressor factors.
Hybridomas produced by fusion between the BW5147 thymoma and an LDH-B-specific B10.A(2R) suppressor T cell line secrete two T suppressor factors (TsF). One factor (TsF-A) shares Mhc determinants with the A alpha A beta molecule and suppresses proliferating Th cells; the other (TsF-E) shares determinants with the E alpha E beta molecule and it inhibits the maturation of the T suppressor (Ts) cells. Here we demonstrate that the two factors can be used to alter the immune response status of cultured T lymphocytes or of an animal. When added to a culture of LDH-B-primed cells or injected into mice, the TsF-A turns responders into nonresponders, presumably by blocking the proliferation of the Th cells. The TsF-E converts nonresponder cultures or mice into responders, presumably by preventing the differentiation of Ts cells. As there are good prospects for obtaining TsF in large quantities and in a highly purified form, this manipulation of the immune response by the deployment of specific factors promises to become an efficient new method of immunotherapy.
D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000276:immunology*; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000502:physiology*; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D013491:Suppressor Factors, Immunologic; D013601:T-Lymphocytes / Q000276:immunology
D000941:Antigens; D008222:Lymphokines; D013491:Suppressor Factors, Immunologic; D007770:L-Lactate Dehydrogenase