title: The resolution of bimodal peaks in the analytical ultracentrifuge in the presence and absence of ligands.
abstract: The patterns for peak resolution in the analytical ultracentrifuge characteristic of systems associating by different mechanisms are analyzed. These are (i) that in which reequilibration is infinitely slow; (ii) that in which reequilibration is rapid, i.e., a Gilbert-type system; and (iii) that in which reequilibration is rapid, but the self-association is mediated by a ligand. Criteria for distinguishing the three are described and illustrated by results on tubulin self-association under various conditions.
mesh_terms: D002021:Buffers; D008024:Ligands; D011108:Polymers; D014404:Tubulin / Q000032:analysis; D014461:Ultracentrifugation / Q000295:instrumentation / Q000379:methods*
chemical_list: D002021:Buffers; D008024:Ligands; D011108:Polymers; D014404:Tubulin