Enzyme-linked immunoadsorbent assay for detection of colony-stimulating factor (CSF-1).
An enzyme-linked immunoadsorbent assay (ELISA) has been developed for the quantitative measurement of L-cell colony-stimulating factor (CSF-1). According to this method, purified antigen (CSF-1) was allowed to adsorb to polystyrene or polyvinyl microtiter wells, which were then incubated with specific rat anti-CSF-1 antiserum with or without standard antigen or unknown test samples. The amount of antibody bound to the solid phase was then quantitated by enzyme (horseradish peroxidase)-conjugated rabbit anti-rat immunoglobulin antibody. This assay has a sensitivity comparable to that of the bioassay (approximately 5 U) and can be carried out in a single day.
D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D001853:Bone Marrow / Q000032:analysis*; D001854:Bone Marrow Cells; D003115:Colony-Stimulating Factors / Q000032:analysis* / Q000276:immunology; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007106:Immune Sera / Q000032:analysis; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011863:Radioimmunoassay; D051381:Rats
D003115:Colony-Stimulating Factors; D007106:Immune Sera