[Study of the nature of increased weight of lymphocyte superhelical DNA during cell incubation in vitro].
The nature of the increased sedimentation of superhelical DNA (sDNA) during incubation of both intact and irradiated thymocytes has been studied. The "weighting" of sDNA was not consequent on the changes in superhelical density. No quantitative or qualitative changes in the composition of proteins connected with sDNA in the course of its "weighting" were found either. It is assumed that sDNA "weighting" seen during incubation of the cells exposed to non-physiological factors (irradiation, hyperthermia, incubation in a poor medium) reflects the processes of chromatin condensation followed by the apoptic death of lymphoid cells.
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D004278:DNA, Superhelical / Q000378:metabolism* / Q000528:radiation effects; D008297:Male; D051379:Mice; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000378:metabolism*
D004278:DNA, Superhelical
Issledovanie prirody "utiazheleniia" superspiral'noĭ DNK limfotsitov v protsesse inkubatsii kletok in vitro.