Production of type I (alpha/beta) interferon after virus infection of cloned, alloantigen-sensitized mouse T lymphocytes.
Mouse T lymphocytes sensitized to alloantigens were cloned by limiting dilution in the presence of interleukin 2. Clones were tested for surface markers Thy-1, Lyt-1 and Lyt-2, and for cytotoxic function. Production of interferon (IFN) by clones either (a) stimulated with allogeneic cells; (b) activated with concanavalin A (Con A); or (c) infected with Semliki Forest virus or Newcastle disease virus were assayed. All clones produced IFN upon Con A stimulation and most after virus infection. Analysis of the IFN produced by a single clone, using anti-IFN antisera, showed that while Con A stimulation induced production of type II IFN (IFN-gamma), the IFN produced after virus infection was type I IFN (IFN-alpha/beta).
D000818:Animals; D002450:Cell Communication; D002472:Cell Transformation, Viral; D002999:Clone Cells; D003208:Concanavalin A / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral; D003601:Cytotoxicity Tests, Immunologic; D007114:Immunization; D007370:Interferon Type I / Q000096:biosynthesis*; D051379:Mice; D012672:Semliki forest virus / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000502:physiology
D007370:Interferon Type I; D003208:Concanavalin A