A reliable and quick radial thin layer chromatographic method for the diagnosis of mono- and disaccharide disorders.
The authors propose a radial thin layer chromatographic method for the separation of mono- and disaccharides, which shows better resolution, and is more sensitive (0.25 microgram for glucose) and less time-consuming (20 min) than the linear technique. Moreover, the procedures used for the detection of sugars and their dansyl-derivatives (staining with naphthoresorcinol and fluorescence of the dansyl-hydrazones) make the identification more specific and reliable. This simple method is quite suitable for the analysis of biological samples (urine, faeces) and the diagnosis of metabolic disorders in children.
D002239:Carbohydrate Metabolism, Inborn Errors / Q000175:diagnosis*; D002855:Chromatography, Thin Layer / Q000379:methods*; D004058:Diffusion; D004187:Disaccharides / Q000032:analysis / Q000652:urine; D005243:Feces / Q000032:analysis; D006801:Humans; D009005:Monosaccharides / Q000032:analysis / Q000652:urine
D004187:Disaccharides; D009005:Monosaccharides