Identification of the heat shock band 2-48B of Drosophila hydei and determination of its haploid DNA content.
The heat shock locus 2-48B situated at the tip of the second chromosome of Drosophila hydei has been studied by various cytologic methods. Both from puffing behaviour and in situ hybridization on both wildtype animals and heterozygotes for the mutant chromosome Df(2)e20 the actual site is localized in band 2-48B8. Electron microscopically this band appears to be a medium size, dotted band. From cytophotometric measurements on Feulgen stained chromosomes and electron microscopic observations the DNA content of band 2-48B8 is calculated to be approximately 40 kb on the haploid level, with a compaction of the DNA of about 160 times. The interbands 2-48B7-8 and 2-48B8-9, flanking the heat shock band, were calculated to contain on the haploid level 1.5 kb and 3.0 kb, respectively. The results are discussed also in relation to the present data on the molecular organization of this locus.
D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes / Q000032:analysis* / Q000648:ultrastructure; D004247:DNA / Q000032:analysis*; D004330:Drosophila / Q000235:genetics*; D005796:Genes; D006238:Haploidy; D006360:Heat-Shock Proteins / Q000235:genetics*
D006360:Heat-Shock Proteins; D004247:DNA