Extraction of a melanoma growth-stimulatory activity from culture medium conditioned by the Hs0294 human melanoma cell line.
Extracts of conditioned medium (CM) from Hs0294 human malignant melanoma cells stimulate [3H]thymidine incorporation and an increase in cell number in serum-depleted Hs0294 cells. This activity is acid and heat stable, nonproteolytic, protease sensitive, contains disulfide bonds and elutes broadly from a Bio-Gel P-30 column with an approximate molecular weight range of 6,000 to 25,000. Hs0294 CM also stimulates [3H]thymidine incorporation in nonmalignant human nevus cells and normal rat kidney fibroblast cells but not in human fibroblasts. There was only limited competition with 125I-epidermal growth factor in binding assays. Hs0294 CM extracts stimulate anchorage-independent growth in normal rat kidney fibroblast cells in soft agar but not in Hs0294 cells, nevus cells, or human fibroblasts. This second activity elutes from the Bio-Gel P-30 column in two positions with apparent molecular weights of 27,000 and 11,000.
D000362:Agar; D000818:Animals; D002452:Cell Count; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media / Q000032:analysis; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006133:Growth Substances / Q000302:isolation & purification*; D006801:Humans; D007668:Kidney / Q000378:metabolism / Q000648:ultrastructure; D008545:Melanoma / Q000378:metabolism*; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000494:pharmacology; D009506:Nevus / Q000378:metabolism; D051381:Rats
D003470:Culture Media; D006133:Growth Substances; D009414:Nerve Growth Factors; D004815:Epidermal Growth Factor; D000362:Agar; D004247:DNA