title: Degradation of aniline and monochloroanilines by Rhodococcus sp. An 117 and a pseudomonad: a comparative study.
abstract: Two newly isolated aniline-degrading bacterial strains were characterized with regard to their enzyme systems responsible for aniline catabolism. One of them identified as a Rhodococcus sp. metabolized aniline exclusively via the beta-ketoadipate pathway by means of inducible enzymes. The aniline-degrading enzyme system of the second isolate, presumably a pseudomonad, was shown to consist of an inducible aniline-converting enzyme and constitutive meta-pathway enzymes. Both isolates failed to metabolize monochlorinated anilines in the absence of additional carbon sources. To explain this the ring-cleaving enzymes of both isolates were examined for their substrate specificities. Furthermore, the effect of 4-chlorocatechol on the enzymes catalyzing aniline conversion and catechol oxygenation was investigated.
mesh_terms: D000192:Actinomycetales / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D000814:Aniline Compounds / Q000378:metabolism*; D001673:Biodegradation, Environmental / Q000187:drug effects; D002396:Catechols / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007700:Kinetics; D011549:Pseudomonas / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D013379:Substrate Specificity / Q000187:drug effects
chemical_list: D000814:Aniline Compounds; D002396:Catechols; D004791:Enzyme Inhibitors; C038861:4-chlorocatechol; C023650:aniline