Heterogeneity of infection enhancement of dengue 2 strains by monoclonal antibodies.
Seven dengue (DEN) 2 virus strains were studied for antibody-dependent enhancement (ADE) of infection in P388D1 mouse macrophage-like cells by using a panel of five DEN 2 monoclonal antibodies. DEN 2 strains were of diverse temporal, geographic, and disease origins. By hemagglutination inhibition and a plaque-reduction neutralization test in LLC-MK2 cells, two of the monoclonal antibodies were type specific and three were flavivirus group reactive. In LLC-MK2 cells, the seven DEN 2 viruses each were neutralized by all five monoclonal antibodies. In P388D1 cells, two DEN 2 strains were enhanced by only three monoclonal antibodies, two by four antibodies, and three by all five antibodies, demonstrating that in some instances enhancement is epitope related and not a concentration-dependent function of virus-antibody interactions. However, ADE did not segregate with determinants exhibiting either the flavivirus group or the dengue type specificity. The presence or absence of enhancement determinants on DEN 2 strains did not correlate with the geographic origin of virus or the severity of disease yielding the strain. The heterogeneous distribution of enhancement determinants may provide a valence mechanism contributing to a multiple increase of infection enhancement in macrophages.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000502:physiology*; D000914:Antibodies, Viral / Q000276:immunology / Q000502:physiology; D000918:Antibody Specificity; D015478:Antigens, Heterophile / Q000276:immunology*; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D003715:Dengue / Q000209:etiology / Q000276:immunology*; D003716:Dengue Virus / Q000145:classification / Q000276:immunology* / Q000472:pathogenicity; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015478:Antigens, Heterophile; D000956:Antigens, Viral