Biochemical analysis of variant HLA-B27 antigens.
Biochemical analysis of HLA-B27 antigens from individuals that are HLA-ABC identical by serology but distinct in CML typing establishes that two types of HLA-B27 can be defined biochemically. The division of HLA-B27 into a W type and a K type by CML typing correlates perfectly with the biochemical data. HLA-B27 K type possesses a more basic isoelectric point than HLA-B27 W type. Neuraminidase digestions of immunoprecipitated HLA-A,B antigens establish that the difference in isoelectric point between HLA-B27 K and HLA-B27 W is not due to differences in sialic acid content.
D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D006680:HLA Antigens / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D015796:HLA-B27 Antigen; D006801:Humans; D009439:Neuraminidase; D011233:Precipitin Tests; D012794:Sialic Acids / Q000032:analysis
D006680:HLA Antigens; D015796:HLA-B27 Antigen; D012794:Sialic Acids; D009439:Neuraminidase