title: The effect of chlorpromazine and dibucaine on phospholipid metabolism in the frog sartorius muscle.
abstract: The effect of cationic amphiphilic drugs, chlorpromazine and dibucaine, on phospholipid metabolism in the frog sartorius muscle was studied at concentrations affecting excitation-contraction coupling. Labelling patterns of phospholipids using 32P orthophosphate were determined. The drugs at a concentration of 3.10(-4) mol/l inhibited the synthesis of phosphatidylcholine and phosphatidylethanolamine and stimulated the synthesis of phosphatidylinositol and phosphatidylserine. At the 3.10(-3) mol/l concentration the drugs blocked the synthesis of all phospholipids without appreciably affecting their degradation. The effect of cationic amphiphilic drugs was independent of the presence of Ca2+ or K+ ions in the media, however, basal labelling of phospholipids was affected by withdrawal of Ca2+ or K+ ions.
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002746:Chlorpromazine / Q000494:pharmacology*; D003992:Dibucaine / Q000494:pharmacology*; D066298:In Vitro Techniques; D009132:Muscles / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism; D010718:Phosphatidylserines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D011896:Rana temporaria
chemical_list: D010716:Phosphatidylinositols; D010718:Phosphatidylserines; D010743:Phospholipids; D003992:Dibucaine; D002118:Calcium; D002746:Chlorpromazine