Estrogen-induced creatine kinase in the reproductive system of the immature female rat.
An increase in the biosynthetic rate of the brain-type isozyme of creatine kinase (CKBB, first described in the uterus as the "estrogen-induced protein") was found in the ovary, vagina and estrogen receptor-rich regions of the brain (preoptic area, anterior hypothalamus and median eminence), one hour after injection of 5 micrograms of estradiol-17 beta into 25-28 day-old rats. The increase in synthetic rate in the ovary, detected by 35S methionine incorporation, peaked at 1h, but still remained higher than in control ovaries at 6 h and was reflected in a longer-term increase in ovarian CK specific activity after 4 daily injections. Both ovary and vagina, similarly to brain, contained exclusively the BB isozyme of CK. These findings suggest that the entire female reproductive system can respond to estrogen by a rapid induction of CKBB.
D000818:Animals; D001921:Brain / Q000187:drug effects; D003402:Creatine Kinase / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005836:Genitalia, Female / Q000201:enzymology*; D007527:Isoenzymes; D010053:Ovary / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D014621:Vagina / Q000187:drug effects
D007527:Isoenzymes; D004958:Estradiol; D003402:Creatine Kinase