Different growth factors stimulate cell division of rat mammary epithelial, myoepithelial, and stromal cell lines in culture.
A number of single-cell-cloned cell lines have been used to examine the growth-promoting effects of putative mammotrophic agents on the various cell types in normal and neoplastic rat mammary glands. A partially purified novel pituitary-derived growth factor stimulates only cuboidal epithelial cells to divide whereas fibroblast growth factor (FGF) stimulates the growth of stromal and myoepithelial-like cells. Epidermal growth factor (EGF) has a widespread but variable growth-stimulating action, but prolactin and growth hormone are essentially inactive when added alone at a concentration of 5 micrograms/ml. Phosphoethanolamine stimulates the growth of one epithelial cell line and a derivative myoepithelial-like cell line, but is inactive on the other cell lines tested. The use of defined cloned cell lines provides a direct and reproducible assay for the identification and purification of inducers of mammary growth.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells; D004983:Ethanolamines / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D008321:Mammary Glands, Animal / Q000166:cytology*; D010907:Pituitary Hormones / Q000494:pharmacology; D051381:Rats
D004983:Ethanolamines; D006133:Growth Substances; D010907:Pituitary Hormones; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; C005448:phosphorylethanolamine