Accumulation of alpha-ketoisocaproate from leucine in homogenates of tissues from rainbow trout (Salmo gairdnerii) and rat. An improved method for determination of branched chain keto acids.
The dinitrophenyl hydrazone method for determination of branched chain keto acids has been improved by the inclusion of a chromatographic purification step. The dinitrophenyl hydrazone of the keto acid was purified by high pressure liquid chromatography on a reversed phase column, with methanol/acetate buffer as the eluting solvent. Kidney and dark muscle of rainbow trout had very high levels of leucine-alpha-ketoglutarate aminotransferase, while the level was low in liver and white muscle.
D000818:Animals; D007651:Keto Acids / Q000032:analysis* / Q000096:biosynthesis*; D007668:Kidney / Q000378:metabolism; D007930:Leucine / Q000378:metabolism*; D051276:Leucine Transaminase; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains / Q000378:metabolism; D012487:Salmonidae / Q000378:metabolism*; D013045:Species Specificity; D000637:Transaminases / Q000378:metabolism; D014337:Trout / Q000378:metabolism*
D007651:Keto Acids; C013082:alpha-ketoisocaproic acid; D000637:Transaminases; D051276:Leucine Transaminase; D007930:Leucine