A single column method to measure hydrogen--tritium exchange by gel chromatography.
Employment of a commercially integrated gel chromatography system together with the utilization of cross-linked polyacrylamide as the chromatographic medium simplifies the methodology of hydrogen--tritium exchange measurements. The system described allows the execution of hydrogen--tritium exchange measurements with as little as 0.5 mg protein per time point and with only a single pass of sample through the column for out-exchange times of less than 1 min to at least 24 h. The accuracy and precision of this system are comparable to those of existing methodologies.
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel / Q000379:methods*; D005293:Ferritins; D006859:Hydrogen; D014316:Tritium
D014316:Tritium; D006859:Hydrogen; D005293:Ferritins