Glucuronic acid-containing glycopeptide from squid cartilage.
A glycopeptide fraction containing glucuronic acid as a component sugar was extracted and purified from squid cartilage to give a single band migrating much slower than hyaluronic acid in cellulose acetate electrophoresis. The molecular weight of the glycopeptide was fairly large since its Kav value in Sephadex G-200 chromatography was 0.18; however, it was soluble in 66% ethanol. This glycopeptide contained glucuronic acid, glucosamine, galactosamine, galactose, and fucose. The total amino acid content was 1.87 mumol of amino acid per mg of the glycopeptide. Threonine, serine and proline represented 80% of the amino acids. Digestion with chondroitinase ABC or reaction with nitrous acid did not result in degradation of the glycopeptide; however, it was completely degraded by reaction with 0.5 M KOH at 37 degrees C. Two hexasaccharides were separated from the alkaline degradation products, and they both contained glucuronic acid, fucose, galactosamine, and reducing terminal glucosamine in the molar ratio, 2:1:2:1. These results indicated that the glycopeptide contains glucuronic acid-containing sugar chains that are distinct from any known glycosaminoglycan.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002356:Cartilage / Q000032:analysis*; D002855:Chromatography, Thin Layer; D049832:Decapodiformes; D005965:Glucuronates / Q000032:analysis*; D020723:Glucuronic Acid; D006020:Glycopeptides / Q000302:isolation & purification*; D009844:Oligosaccharides / Q000302:isolation & purification
D000596:Amino Acids; D002241:Carbohydrates; D005965:Glucuronates; D006020:Glycopeptides; D009844:Oligosaccharides; D020723:Glucuronic Acid