Secretory protein synthesis in parotid glands of young and old rats.
To determine whether the synthesis of secretory proteins changes with age, the incorporation of [14C]-leucine into exportable proteins of the parotid gland was compared in 2- and 24-month-old rats. The proteins were separated by SDS-gel electrophoresis of the post-microsomal supernatant and identified by comparing the banding patterns in gels prepared from unstimulated glands with those from the glands stimulated to secrete. The amount of radioactivity incorporated into the bands corresponding to exportable proteins was significantly less in the older group, indicating that the synthesis of secretory proteins declines with age.
D000375:Aging; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D010306:Parotid Gland / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012471:Salivary Proteins and Peptides / Q000096:biosynthesis*; D000516:alpha-Amylases / Q000096:biosynthesis
D012471:Salivary Proteins and Peptides; D000516:alpha-Amylases; D007545:Isoproterenol