Adsorption and reactivity of human fibrinogen and immunoglobulin G on two types of hemodialysis membranes.
Phase contrast microscopic examination of Cuprophane and polyacrylonitrile. After clinical ex vivo use by uremic patients revealed extensive cellular deposition of erythrocytes, platelets, and leukocytes on Cuprophane but not on polyacrylonitrile. In vitro studies with 125I-labeled human fibrinogen or immunoglobulin G (IgG) showed that the adsorption of fibrinogen or IgG was greater on polyacrylonitrile than on Cuprophane. Further studies on the reactivity of fibrinogen adsorbed on polyacrylonitrile surface indicated that the absorbed fibrinogen: (a) was not desorbed readily from the surface, (b) was not appreciably displaced by other plasma proteins such as albumin, IgG, and fibrinogen, (c) was not readily accessible for reaction with 125I-antifibrinogen-IgG, and (d) did not promote the adhesion of 51Cr-labeled platelets. IgG adsorbed on the surface produced essentially no effect on platelet adhesion to polyacrylonitrile and promoted only slightly on the adhesion of granulocytes to the same material. On the other hand, fibrinogen and IgG augmented greatly the platelet adhesion to Cuprophane and IgG enhanced the granulocyte adhesion moderately. These data indicate that fibrinogen and IgG, though at high concentration on polyacrylonitrile, may adsorb in a biologically inactive form. Our observations suggest that the thrombogenicity of an artificial surface may not be assessed entirely by the types and amount of the various protein species adsorbed but is likely determined by the reactivity of the specific protein species adsorbed on the material.
D000042:Absorption; D000180:Acrylic Resins; D001792:Blood Platelets / Q000378:metabolism; D002482:Cellulose / Q000031:analogs & derivatives; D005340:Fibrinogen / Q000378:metabolism* / Q000494:pharmacology; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D008567:Membranes, Artificial; D010973:Platelet Adhesiveness / Q000187:drug effects; D006435:Renal Dialysis
D000180:Acrylic Resins; D007074:Immunoglobulin G; D008567:Membranes, Artificial; C010504:polyacrylonitrile; D005340:Fibrinogen; D002482:Cellulose; C009865:cuprammonium cellulose