High-performance gel-permeation chromatography of polypeptides in a volatile solvent: rapid resolution and molecular weight estimations of proteins and peptides on a column of TSK-G3000-PW.
A gel-permeation column of TSK-G3000-PW that was equilibrated and developed with 36 or 45% acetonitrile in 0.1% trifluoroacetic acid fractionated mixtures of peptides with high resolving power. In addition, the elution volumes of 11 standard peptides and proteins were linearly related to the logarithms of their molecular weights in the acetonitrile-trifluoroacetic acid solvent at both low and high flow rates. Since the solvent is volatile and relatively transparent to short-wavelength ultraviolet light this high-performance gel-permeation system offers a rapid and highly sensitive method for the analysis, characterization, and purification of peptides and proteins from complex mixtures.
D000097:Acetonitriles; D002850:Chromatography, Gel; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification*; D011506:Proteins / Q000032:analysis; D012997:Solvents; D014269:Trifluoroacetic Acid
D000097:Acetonitriles; D010455:Peptides; D011506:Proteins; D012997:Solvents; D014269:Trifluoroacetic Acid