Origin of peripheral blood macrophages in bursa-cell-reconstituted chickens. Further evidence of MHC-restricted interactions between T and B lymphocytes.
MHC class-I antigens on peripheral blood macrophages and bursa cells were analysed after adoptive bursa cell transfer to cyclophosphamide-treated immunodeficient chickens. We studied the expression of B-F locus-encoded antigens (B-F is homologous to mouse H-2K, D) on macrophages and B cells and found that macrophage B-F antigens are of host origin, whereas bursa cells express only donor-type B-F antigens. Both syngeneic and allogeneic bursa cells restored IgM antibody production to a T-cell-independent antigen, Brucella, but only syngeneic bursa cells could restore the IgG antibody response to a thymus-dependent antigen, sheep erythrocytes. The results indicate that B-cell maturation and macrophage-B-cell interaction are not MHC-restricted but that a restriction exists in T-cell-B-cell collaboration.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D001402:B-Lymphocytes / Q000276:immunology*; D002060:Bursa of Fabricius / Q000166:cytology / Q000276:immunology / Q000637:transplantation; D002645:Chickens; D003520:Cyclophosphamide / Q000494:pharmacology; D006410:Hematopoiesis; D006649:Histocompatibility Antigens / Q000032:analysis / Q000276:immunology*; D007116:Immunization, Passive; D008211:Lymphocyte Cooperation; D008264:Macrophages / Q000166:cytology*; D013601:T-Lymphocytes / Q000276:immunology*
D006649:Histocompatibility Antigens; D003520:Cyclophosphamide