title: Diphenhydramine determination in human plasma by gas-liquid chromatography using nitrogen-phosphorus detection: application to single low-dose pharmacokinetic studies.
abstract: A highly sensitive method (less than or equal to 1 ng/ml) for single-dose pharmacokinetic studies of diphenhydramine which utilizes GLC with nitrogen-phosphorus detection is described. Standard curves, using orphenadrine as the internal standard, were linear for diphenhydramine concentrations from 1.0 to 300 ng/ml. Applicability of the method was demonstrated by a pharmacokinetic study in a normal volunteer who received 25 mg iv of diphenhydramine.
mesh_terms: D000328:Adult; D002849:Chromatography, Gas / Q000379:methods; D004155:Diphenhydramine / Q000008:administration & dosage / Q000097:blood*; D006801:Humans; D007700:Kinetics; D008297:Male
chemical_list: D004155:Diphenhydramine