[Effect of glycodihydrofusidate, a structural analog of bile salts, on the hepatic transport of bromosulfophthalein in rats].
The interaction between bromosulfophthalein and glycodihydrofusidate in their transport by the liver were studied. In vitro, glycodihydrofusidate, a bile salt analogue, inhibited bromosulfophthalein uptake by isolated rat liver cells. This inhibition was similar to that previously described by adding sodium taurocholate to the medium; the inhibition was only partial and could no longer be detected at high bromosulfophthalein concentrations (20 microM). These results suggest that glycodihydrofusidate, like sodium taurocholate can compete with bromosulfophthalein for a common carrier in the liver cell membrane. In vivo, in the rat submitted to a saturating infusion of bromosulfophthalein, the addition of glycodihydrofusidate to the perfusate induced a 65 p. 100 decrease in the biliary excretion of bromosulfophthalein, a decrease in the water flow (47 p. 100) and a slight diminution in the bile salt output (14 p. 100). In experiments where glycodihydrofusidate-bromosulfophthalein interactions did not occur at the sinusoidal level, the biliary excretion of the dye was inhibited by glycodihydrofusidate. This suggests a common pathway for the two molecules. Our results are consistent with the existence of two different bromosulfophthalein carrier systems present at either pole of the hepatocyte. However only one is shared with bile salts and glycodihydrofusidate. This same hypothesis might account for many other experimental results as well.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D005672:Fusidic Acid / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013448:Sulfobromophthalein / Q000378:metabolism*
C013544:glycodihydrofusidate; D013448:Sulfobromophthalein; D005672:Fusidic Acid
Influence du glycodihydrofusidate, un analogue structural des sels biliaires, sur le transport hépatique de la bromesulfonephtaléine chez le rat.