Partial amino terminal sequence of the precursor of mitochondrial ATPase inhibitor protein synthesized with mRNA partially purified by gel permeation chromatography.
Messenger RNA coding mitochondrial ATPase inhibitor protein, a small peptide comprised of 63 amino acid residues, was separated from a large quantity of mRNAs of larger molecules by high speed gel permeation chromatography. Messenger RNA coding a small stabilizing factor of inactivated F1F0-ATPase complex, which is also comprised of 63 amino acids, was recovered in the same fraction as the ATPase inhibitor, whereas mRNA for a large stabilizing factor with an apparent molecular weight of 15,000 was recovered in a fraction of slightly larger molecules. ATPase inhibitor precursor labeled with various kinds of radioactive amino acids was prepared separately by cell-free translation with the purified mRNA, and the amino terminal sequence of the precursor was examined. It was demonstrated that an extra peptide of 21 amino acid residues, including 5 leucine, 4 serine, 1 glycine, and 1 methionine residues, is located at the amino terminus of the ATPase inhibitor precursor.
D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D066298:In Vitro Techniques; D008928:Mitochondria / Q000378:metabolism*; D008970:Molecular Weight; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis*; D011506:Proteins; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D000097795:ATPase Inhibitory Protein
D011498:Protein Precursors; D011506:Proteins; D012333:RNA, Messenger; D000097795:ATPase Inhibitory Protein