Pulse radiolytic study of the interaction of thiols and ascorbate with OH adducts of dGMP and dG: implications for DNA repair processes.
Using the technique of pulse radiolysis, the interaction of .OH radicals with 2'-deoxyguanosine (dG) and dG-5'-monophosphate (dGMP) has been shown to result in the production of intermediates with different redox properties as demonstrated by their reactions with tetranitromethane (TNM) and N,N,N',N'-tetramethyl-p-phenylenediamine. The ratio of the yields of oxidizing to reducing-type .OH radical adducts of dGMP was determined to be about 1:1 and independent of pH (6-11). The nature of the intermediates produced on reaction of .OH with dGMP are discussed. The thiols, cysteine, glutathione, mercaptoacetic acid, and ascorbate have been shown to interact with those .OH adducts of dGMP and dG with oxidizing properties preferentially via an electron transfer process (k approximately 3 X 10(7)-1.4 X 10(9) dm3 mole-1 sec-1) as implied from the pH dependence of the rate constants. It is further demonstrated that oxygen and TNM do not interact with those .OH adducts of the purines with oxidizing properties. The implications of these findings are discussed with reference to the mechanistic aspects of radioprotection and especially of radiosensitization.
D001205:Ascorbic Acid / Q000528:radiation effects*; D004260:DNA Repair / Q000528:radiation effects*; D003848:Deoxyguanine Nucleotides / Q000528:radiation effects*; D003849:Deoxyguanosine / Q000528:radiation effects*; D004347:Drug Interactions / Q000528:radiation effects; D005609:Free Radicals; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides / Q000528:radiation effects*; D010084:Oxidation-Reduction / Q000528:radiation effects; D010315:Particle Accelerators; D011675:Pulse Radiolysis; D011836:Radiation Tolerance; D013438:Sulfhydryl Compounds / Q000528:radiation effects*
D003848:Deoxyguanine Nucleotides; D005609:Free Radicals; D006878:Hydroxides; D013438:Sulfhydryl Compounds; C007257:2'-deoxyguanosine 5'-phosphate; D003849:Deoxyguanosine; D001205:Ascorbic Acid