Induction of interleukin 1 messenger RNA and translation in oocytes.
Macrophages and P388D1 murine cultured cells produce interleukin 1 (lymphocyte-activating factor) when stimulated with lipopolysaccharide or other agents. Poly A+ RNA extracted from either type of stimulated cells and injected into Xenopus oocytes causes synthesis of material with the biologic and biochemical properties of interleukin 1. It potentiates lectin-mediated thymocyte proliferation, and it has the molecular dimensions of interleukin 1, as determined by gel exclusion chromatography. RNA from either cell type causes the synthesis of interleukin 1 with an isoelectric point of 4.8 to 5.0. RNA prepared from unstimulated macrophages or P388D1 cells does not cause interleukin 1 production by oocytes. We conclude that the amount of interleukin 1 mRNA increases greatly after stimulation of either cell type, and oocytes carry out any modifications of the polypeptide necessary for activity. The kinetics of interleukin 1 mRNA accumulation and of interleukin 1 production by macrophages and P388D1 cells are compared.
D000818:Animals; D002460:Cell Line; D005260:Female; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis* / Q000502:physiology; D007700:Kinetics; D008262:Macrophage Activation; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009865:Oocytes / Q000378:metabolism*; D014176:Protein Biosynthesis; D011650:Pulmonary Alveoli / Q000166:cytology; D012333:RNA, Messenger / Q000096:biosynthesis*; D011817:Rabbits
D007375:Interleukin-1; D012333:RNA, Messenger