Optimal conditions for proliferation of bone marrow-derived mouse macrophages in culture: the roles of CSF-1, serum, Ca2+, and adherence.
A method is described for the analysis of [3H]-thymidine incorporation in microtitre cultures of bone marrow-derived mouse macrophage responding to macrophage colony-stimulating factor (CSF-1). [3H]-thymidine incorporation depends on cell density, culture medium, and the concentration of CSF-1 and serum, but is independent of Ca2+. Bone marrow-derived macrophages are strongly adherent, but adherence can be dissociated from [3H]-thymidine incorporation.
D000818:Animals; D001769:Blood; D001853:Bone Marrow / Q000502:physiology; D002118:Calcium / Q000494:pharmacology*; D002448:Cell Adhesion; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000302:isolation & purification / Q000494:pharmacology*; D003470:Culture Media; D007739:L Cells / Q000502:physiology; D008264:Macrophages / Q000502:physiology*; D051379:Mice
D003115:Colony-Stimulating Factors; D003470:Culture Media; D002118:Calcium