title: The possible role of juvenile hormone esterase in the regulation of juvenile hormone titre in the female cockroach Diploptera punctata.
abstract: Juvenile hormone (JH) degradation in vitro and in vivo was studied in the viviparous cockroach Diploptera punctata. In vitro studies with juvenile hormone esterase (JHE) and "general" or l-naphthyl acetate esterase (NAcE) revealed that diethyl-p-nitrophenylphosphate (paraoxon) inhibited both JHE and NAcE activity, but the latter was more sensitive and was completely inhibited at 0.1 mM. NAcE activity was resistant to inhibition with Triton X-100, whereas JHE activity in haemolymph of adult females was inhibited 100% at Triton X-100 concentration of 0.25%. Eighty percent inhibition of JHE activity in vivo was observed following injection of 0.2 microL Triton X-100. In contrast to the previously observed dose-dependant increase in JHE activity. NAcE activity did not increase following treatment of allatectomized females with the JH analogue ethyl-2E,4E-3,7,11-trimethyl-2,4-dodecadienoate (hydroprene). Hydroprene was not catabolized in haemolymph of D. punctata in vitro. The half-life of C16 JH (JH III) in the haemolymph, in vivo, was 1.65 h for day 5 females and 2 h for day 6 females.
mesh_terms: D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D003058:Cockroaches; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005260:Female; D006458:Hemolymph / Q000378:metabolism; D007605:Juvenile Hormones / Q000378:metabolism*; D009283:Naphthol AS D Esterase / Q000378:metabolism
chemical_list: D005231:Fatty Acids, Unsaturated; D007605:Juvenile Hormones; C006481:ethyl-3,7,11-trimethyl-2,4-dodecadienoate; D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases; C038003:juvenile hormone esterase