[Immuno-electron microscopy study of Toxoplasma gondii. II. Study of IgM antibodies].
This paper deals with an immunoelectron microscopic study of the localization of IgM vector on the membrane of Toxoplasma gondii using the antibody at the early period of infection. The primary antibody was IgM antibody obtained from rabbit's serum 11 days after the infection of Beverley strain of Toxoplasma gondii from which IgG was absorbed by protein A containing staphylococcus. Ferritine-labeled antibody against rabbit's IgM was used as the secondary antibody. The surface of normal tachyzoites is smooth with two layered plasma membrane. The outer layer shows a three-layer structure which is not found in the inner membrane. The inner membrane is discontinuous with partially lost portion at the head of the tachyzoites. Structure within plasma include conoid toxonems, dark staining vacuoles and nucleus. By reacting with the ferritin-labeled antibody, ferritin particles were found on all over the circumference of the tachyzoites depositing on the surface of the outer plasma membrane, but few on the inner plasma membrane and organelles. It implies that IgM receptors are localized on the membrane surface similarly with our preceding report.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D014122:Toxoplasma / Q000276:immunology / Q000648:ultrastructure*
D000906:Antibodies; D007075:Immunoglobulin M
Contribution à l'étude de Toxoplasma gondii par immuno-microscopie électronique. II. Etude des anticorps IgM.