High frequency detection of different T-cell subsets in mice by a modified virus plaque assay.
Different T-cell subsets participating in immune responses were detected at a high frequency by a modified virus plaque assay (VPA). By using the modified VPA, different activated T-cell subsets generated in primary immune responses, helper and suppressor T cells participating in antibody formation, and effector T cells involved in the delayed-type hypersensitivity (DTH) reaction were enumerated directly without in vitro antigen stimulation. The frequency of detection in immune systems used was 7.5-17.7 V-PFC/10(3) spleen cells. Although neither helper T cells for antibody formation nor effector T cells for DTH reaction were detected as V-PFC at a high frequency by the original VPA, it was also found in secondary immune response that Lyt 1 positive, antigen-specific helper and effector T-cell subsets, and cyclophosphamide (CY)-resistant precursors were enumerated at a high frequency by the modified VPA when the received in vitro antigen stimulation, and that the proliferative stage of these cells was critical for the development of V-PFC.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology; D003520:Cyclophosphamide / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006968:Hypersensitivity, Delayed; D007156:Immunologic Memory; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010047:Ovalbumin / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology; D010948:Viral Plaque Assay / Q000379:methods
D000941:Antigens; D003520:Cyclophosphamide; D010047:Ovalbumin; D004247:DNA