[NK activity, TCGF production and generation of cell-mediated cytotoxicity in spleen cells from gastric cancer patients].
The in vitro cell-mediated immune reactivities of mononuclear cells separated from spleen (SPL) of gastric cancer patients were studied and, these were compared to those of peripheral blood mononuclear cells (PBL) from the same patient. The level of NK activity of SPL was slightly higher than that of PBL (p less than 0.2, by paired t test), as measured by 51Cr release assay using K562 cells as target. TCGF preparations, generated in cultures of PHA stimulated SPL or PBL, were also compared by quantitative assay. SPL produced in significantly larger amounts than PBL (p less than 0.05). Then, the generation of cell-mediated cytotoxicity in mixed cell culture was investigated in both cell populations. When these cells were cultured with B-lymphoblastoid cell line Raji, SPL had much more capacity to generate cytotoxic cells, compared to PBL (p less than 0.1). Moreover, SPL had significantly increased ability of induce concomitant cytotoxicity during sensitizing to normal allogeneic PBL in mixed lymphocyte culture as compared to PBL (p less than 0.005). These results appeared to demonstrate that SPL from gastric cancer patients had much more increased in vitro cell-mediated reactivities, when compared to those of PBL from these patients.
D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000276:immunology*; D013154:Spleen / Q000276:immunology*; D013274:Stomach Neoplasms / Q000276:immunology*
D007376:Interleukin-2