Isolation of melanotropins and vasopressin from neuro-intermediate lobes of bovine pituitary glands. A simplified procedure.
A simplified procedure for the isolation of alpha-melanotropin, beta-melanotropin and arginine vasopressin from the acid acetone extract of posterior-intermediate lobes of bovine pituitaries has been described. It involves essentially two steps: gel filtration on Sephadex G-10 and high performance liquid chromatography.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001127:Arginine Vasopressin / Q000302:isolation & purification*; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D066298:In Vitro Techniques; D009074:Melanocyte-Stimulating Hormones / Q000302:isolation & purification*; D010902:Pituitary Gland / Q000032:analysis*
D000596:Amino Acids; D001127:Arginine Vasopressin; D009074:Melanocyte-Stimulating Hormones