Purification of human factor VII utilizing O-(diethylaminoethyl)-Sephadex and Sulfopropyl-Sephadex chromatography.
A simple procedure for the large scale purification of unactivated human factor VII is described. The initial steps, common to prior purification methods, include adsorption onto barium citrate, ammonium sulfate fractionation, and DEAE-Sephadex chromatography. Factor VII is isolated in pure unactivated form by one additional step, Sulfopropyl-Sephadex chromatography. Ten liters of plasma yields 1.3 mg of protein representing approximately 30% recovery.
D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005167:Factor VII / Q000032:analysis / Q000302:isolation & purification*; D015942:Factor VIIa; D006801:Humans; D011817:Rabbits
D005167:Factor VII; D015942:Factor VIIa