title: Matrix Gla protein, a new gamma-carboxyglutamic acid-containing protein which is associated with the organic matrix of bone.
abstract: A new protein has been isolated from CaCl2/urea extracts of demineralized bovine bone matrix. This protein has five to six residues of the vitamin K-dependent amino acid, gamma-carboxyglutamic acid (Gla), and we have accordingly designated it matrix Gla protein. Matrix Gla protein is a 15,000 dalton protein whose amino acid composition includes a single disulfide bond. The absence of 4-hydroxyproline in matrix Gla protein demonstrates that it is not a precursor to bone Gla protein, 5,800 dalton protein which has a residue of 4-hydroxyproline at position 9 in its sequence. Matrix Gla protein also does not cross-react with antibodies raised against bone Gla protein.
mesh_terms: D015055:1-Carboxyglutamic Acid / Q000302:isolation & purification*; D000596:Amino Acids / Q000302:isolation & purification; D000818:Animals; D001842:Bone and Bones / Q000032:analysis*; D002135:Calcium-Binding Proteins / Q000302:isolation & purification*; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005971:Glutamates / Q000302:isolation & purification*; D015675:Osteocalcin; D011863:Radioimmunoassay; D012967:Sodium Dodecyl Sulfate
chemical_list: D000596:Amino Acids; D002135:Calcium-Binding Proteins; D005971:Glutamates; D015675:Osteocalcin; D012967:Sodium Dodecyl Sulfate; D015055:1-Carboxyglutamic Acid