Non-deleterious dinitrophenyl (DNP) hapten labelling of antibody protein. Preparation and properties of some short-chain DNP imidoesters.
Methyl 3-(2,4- dinitrophenylamino ) propionimidate hydrochloride (DNP-N-IE), methyl 3-(2,4-dinitrophenyl-N-methylamino) propionimidate hydrochloride (DNP- NMe -IE), and methyl 3-(2,4- dinitrophenylthio ) propionimidate hydrochloride (DNP-S-IE) have been prepared. DNP-N-IE and DNP- NMe -IE both react efficiently at 0-2 degrees C and pH 7-9.5 with sheep immunoglobulin G. At least 12 DNP groups can be introduced into the antibody protein without causing any significant change in its antigen binding affinity or capacity.
D000345:Affinity Labels / Q000138:chemical synthesis / Q000378:metabolism*; D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D001666:Binding Sites, Antibody; D004136:Dinitrobenzenes / Q000378:metabolism*; D006241:Haptens; D006863:Hydrogen-Ion Concentration; D007096:Imidoesters / Q000378:metabolism*; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism*; D007700:Kinetics; D009578:Nitrobenzenes / Q000378:metabolism*; D011817:Rabbits; D012756:Sheep
D000345:Affinity Labels; D000888:Antibodies, Anti-Idiotypic; D004136:Dinitrobenzenes; D006241:Haptens; D007096:Imidoesters; D007074:Immunoglobulin G; D009578:Nitrobenzenes