Effects of deoxycoformycin in mice. I. Suppression and enhancement of in vivo antibody responses to thymus-dependent and -independent antigens.
The effect of 2'-deoxycoformycin (DCF) on the PFC responses of AKR mice to SE, TNP-Ficoll, and TNP-B. abortus was examined. Subcutaneous injection of DCF 4 days before antigen caused suppression of all three responses by 70 to 78%. In contrast, injection of DCF 1 day after antigen caused enhancement of both the anti-SE and the anti-TNP-Ficoll responses. Although a single high dose of cortisone acetate injected 4 days before antigen caused a similar suppression, the effect of DCF was not mediated via a steroid release, inasmuch as DCF also suppressed the immune response in adrenalectomized mice. The response of BALB/c mice to TNP-Ficoll was also inhibited by DCF pretreatment and enhanced by injection of DCF after antigen. In contrast, in athymic mice DCF caused suppression of the anti-TNP-Ficoll PFC response, whether injected before or after antigen. These results are interpreted as suggesting that DCF causes suppression primarily via an effect on B cells. The enhancement seen in normal but not in athymic mice may possibly be ascribed to an effect on suppressor T cells. Apparently the enhancement of both TD and TI responses caused by DCF injected 1 day after antigen in normal mice is the net result of these two opposing effects. The results imply that helper T cells are resistant to DCF.
D000315:Adrenalectomy; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000955:Antigens, T-Independent / Q000008:administration & dosage / Q000276:immunology*; D001789:Blood Group Antigens / Q000276:immunology; D003070:Coformycin / Q000031:analogs & derivatives / Q000494:pharmacology*; D003348:Cortisone / Q000031:analogs & derivatives / Q000494:pharmacology; D004347:Drug Interactions; D005260:Female; D005362:Ficoll / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000276:immunology; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007376:Interleukin-2 / Q000008:administration & dosage / Q000502:physiology; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D015649:Pentostatin; D012263:Ribonucleosides / Q000494:pharmacology*; D012756:Sheep; D014301:Trinitrobenzenes / Q000008:administration & dosage / Q000276:immunology
D000955:Antigens, T-Independent; D001789:Blood Group Antigens; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D012263:Ribonucleosides; C020529:TNP-ficoll; D014301:Trinitrobenzenes; D003070:Coformycin; D005362:Ficoll; D015649:Pentostatin; D003348:Cortisone