The effect on epidermal DNA synthesis of a combination of topical steroid with either dithranol or tar as used for psoriasis.
The hairless mouse was used to investigate the effects of a combination of glucocorticosteroid with either dithranol or tar on epidermal DNA synthesis, in order to determine whether such combinations reduce epidermal DNA synthesis more effectively than the single agents. Dithranol alone produced a significant local inhibition of DNA synthesis at concentrations of 0.1% and 0.05% but not at lower concentrations. Dose-response data for dilutions of clobetasol propionate and betamethasone 17-valerate showed progressive diminution of both local and systemic effects with decreasing concentrations. An additive effect was found from combining clobetasol propionate with dithranol and from combining betamethasone 17-valerate with liquor picis carbonis. These combined preparations were tested again after storage for 6 months and 2 months respectively and showed no loss of efficacy. These results lend justification to the use of these combined preparations in the treatment of psoriasis.
D000818:Animals; D000873:Anthracenes / Q000494:pharmacology*; D000875:Anthralin / Q000494:pharmacology*; D001623:Betamethasone / Q000031:analogs & derivatives*; D001624:Betamethasone Valerate / Q000494:pharmacology*; D002990:Clobetasol / Q000494:pharmacology; D003033:Coal Tar / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D004338:Drug Combinations; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008812:Mice, Hairless
D000873:Anthracenes; D004338:Drug Combinations; D003033:Coal Tar; D004247:DNA; D001623:Betamethasone; D001624:Betamethasone Valerate; D002990:Clobetasol; D000875:Anthralin