Decreased oxygen supply enhances growth in culture of human mid-trimester amniotic fluid cells.
Human mid-trimester amniotic fluid cells were cultivated under conditions of decreased oxygen supply. Compared to control cultures the low-oxygen group showed improved growth which was quantitated by three independent assays (1) direct cell counts, (2) bromodeoxyuridine (BrdU)-Hoechst flow-cytometry, and (3) cloning efficiency. The growth promoting effects of lowered oxygen hold for all major morphologic categories of amniotic fluid cells.
D000653:Amniotic Fluid / Q000166:cytology*; D002452:Cell Count; D002455:Cell Division; D002478:Cells, Cultured; D002999:Clone Cells; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D010100:Oxygen / Q000502:physiology*; D011247:Pregnancy; D011263:Pregnancy Trimester, Third
D010100:Oxygen