Nucleoside triphosphate specificity of firefly luciferase.
Twelve naturally occurring nucleoside triphosphates have been examined as substrates and inhibitors of the light-producing reaction of firefly luciferase. Deoxyadenosine 5'-triphosphate was 1.7% as effective relative to ATP as a substrate, whereas all others tested were less than 0.1% as effective as ATP. At concentrations normally present in mammalian cell extracts no interference with ATP measurements results from these nucleotides.
D000255:Adenosine Triphosphate / Q000032:analysis*; D001517:Coleoptera; D008027:Light; D008156:Luciferases / Q000037:antagonists & inhibitors*; D009705:Nucleosides; D013379:Substrate Specificity
D009705:Nucleosides; D000255:Adenosine Triphosphate; D008156:Luciferases