title: Relationship of germinal centers in lymphoid tissue to immunologic memory. VI. Transfer of B cell memory with lymph node cells fractionated according to their receptors for peanut agglutinin.
abstract: We isolated germinal center B cells by exploiting their high affinity for peanut agglutinin (PNA). The PNA+ and PNA- B cells, fractionated by panning on PNA-coated petri dishes, were examined for their ability to transfer memory responses to irradiated recipients at various times after priming. With such fractionated B cells from lymph nodes taken at the peak of germinal center formation, the largest response was obtained in recipients of the PNA+ B cell population. At 4 to 5 wk after priming, and 10 days after challenge with an unrelated antigen, memory responses were approximately equal in recipients of PNA+ or PNA- B cells. At 14 wk after priming, memory responses were found only in recipients of the PNA- B cell population. Memory B cells from the spleen, taken from mice primed in the footpad 8 wk earlier, were also PNA-. Finally, we show that boosting with a TNP-conjugate in the footpad, 6 mo after priming in the same footpad, induced the reappearance of marked memory responsiveness in the PNA+ B cell fraction of the draining node.
mesh_terms: D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002469:Cell Separation; D006433:Hemocyanins; D007116:Immunization, Passive; D007156:Immunologic Memory; D037102:Lectins / Q000276:immunology / Q000494:pharmacology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D019887:Peanut Agglutinin; D011975:Receptors, Mitogen / Q000032:analysis*; D014301:Trinitrobenzenes / Q000008:administration & dosage
chemical_list: D000941:Antigens; D037102:Lectins; D019887:Peanut Agglutinin; D011975:Receptors, Mitogen; D014301:Trinitrobenzenes; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin