Stereospecific high-performance liquid chromatographic analysis of warfarin in plasma.
A stereospecific high-performance liquid chromatographic assay has been developed to determine R(+)- and S(-)-warfarin simultaneously in plasma. The method involved the formation of diastereoisomeric esters, using carbobenzyloxy-L-proline, with subsequent separation using silica as the stationary phase. The method permits characterization of the pharmacokinetics of warfarin enantiomers following administration of racemic drug.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007700:Kinetics; D013237:Stereoisomerism; D014859:Warfarin / Q000097:blood*
D014859:Warfarin