Determination of amylase by measurement of enzymatic activity and by enzyme immunoassay and radioimmunoassay.
Alpha-amylase content was determined by enzyme immunoassay and radioimmunoassay and enzyme activity by enzymatic test in some pig tissues and serum during ontogenetic development. Among the studied organs, the highest enzyme content and activity were found in the pancreas. In this organ, amylase ratio in fetuses, newborns and adults was 1:13:1500. The ratio of the adult amylase content in pancreas, parotid gland and kidney was 4000:5:1, respectively. Close correlation was shown between the results obtained with the immunological and enzymatic methods.
D000367:Age Factors; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008297:Male; D011863:Radioimmunoassay; D013552:Swine; D014018:Tissue Distribution; D000516:alpha-Amylases / Q000032:analysis* / Q000276:immunology
D000516:alpha-Amylases