Rapid estimation of soluble fibrin monomer complexes by high performance liquid chromatography for the purpose of early detection of DIC.
Hypercoagulability and DIC are characterized by the presence of SFMC in plasma. We have devised a more rapid method with a quantitative estimation of SFMC by use of HPLC. The normal percentage of SFMC of total fibrinogen content was 2.2 +/- 0.8% in plasma. On the other hand, the amount of SFMC in DIC patients showed a level of 6.8% to 16.7%. The level of SFMC in the third postoperative day after gastrointestinal surgery was between 2.3% and 6.1%. These moderately elevated SFMC levels were suggested to be due to the state of hypercoagulability. Thus, the measurement of SFMC with HPLC is a very useful method to analyze hypercoagulability and DIC.
D002851:Chromatography, High Pressure Liquid; D004211:Disseminated Intravascular Coagulation / Q000097:blood* / Q000175:diagnosis; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis*; D006801:Humans
D005338:Fibrin Fibrinogen Degradation Products; C011468:fibrinmonomer