Isotope effects of tritium in a ligand-antibody binding.
The affinity of testosterone labelled with 1, 2, 4 or 6 tritium atoms to testosterone antisera was examined. Scatchard plots indicated an increase of equilibrium constants with the number of tritium atoms per testosterone molecule. This behaviour was observed with all 3 antisera tested. It is speculated that the increase of affinity is due to a decrease of the amplitude of vibrations of tritium atoms, resulting in a decrease of vibrational repulsions.
D000915:Antibody Affinity; D007106:Immune Sera; D013739:Testosterone / Q000276:immunology*; D014316:Tritium
D007106:Immune Sera; D014316:Tritium; D013739:Testosterone