The rate of utilization of glucose via hexosemonophosphate shunt in brain.
The concentration of 6-phosphogluconate in the brain increased from 0-24 nmol/g in the controls to 1430 and 1506 nmol/g in rats treated with 50 mg of 6-aminonicotinamide/kg of body weight. A dose-dependent increase in the concentrations of glucose and glucose 6-phosphate as well as of 6-phosphogluconate was found in the brains of 6-aminonicotinamide-treated rats. The biochemical changes and symptoms of neurological disorder in 6-aminonicotinamide-treated rats were not due to hypothermia. The rate of utilization of glucose via the hexosemonophosphate shunt was determined by isolation of gluconate from 6-phosphogluconate and measurement of its [14C]content at short time intervals after injection of [U-14C]glucose into 6-aminonicotinamide-treated rats; it was 16.5 nmol of glucose utilized/min per g of brain, and represented approximately 2.3% of the overall utilization of glucose in the brain. A highly significant correlation was observed between the concentration of 6-phosphogluconate and the concentration of glucose 6-phosphate and free glucose. The validity of this correlation was supported by the results of previous investigations involving several other treatments.
D015120:6-Aminonicotinamide / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D005942:Gluconates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000378:metabolism; D006600:Hexosephosphates / Q000378:metabolism*; D008297:Male; D010734:Phosphogluconate Dehydrogenase / Q000037:antagonists & inhibitors; D051381:Rats
D005942:Gluconates; D005958:Glucosephosphates; D006600:Hexosephosphates; D015120:6-Aminonicotinamide; D019298:Glucose-6-Phosphate; D010734:Phosphogluconate Dehydrogenase; D005947:Glucose; C008884:6-phosphogluconic acid