Connective tissue activation. XXVIII. A connective tissue activating peptide from human urine.
A protein factor in human urine which has the ability to activate connective tissue cells has been identified and partially purified; it appears to be different from epidermal growth factor and IgG. This urinary connective tissue activating factor (CTAP-U) is nondialyzable, labile to protease, stable to thiols, heat, and acid, and has an acidic isoelectric point. Purified preparations of CTAP-U have biologic activities that cause human connective tissue cells to synthesize incremental amounts of 14C-hyaluronic acid, 35S-proteoglycans, and 3H-DNA in vitro. The cell spectrum responsive to this substance includes human synovial cells, human chondrocytes, and skin fibroblasts. CTAP-U does not react with antisera to connective tissue activating peptide-III or to antibodies against IgG or its Fc and Fab fragments. Furthermore, CTAP-U does not cross-react in a radioreceptor assay for insulin, basic somatomedin, or epidermal growth factor-urogastrone. Utilizing standardized isolation conditions, CTAP-U preparations with these properties have been isolated from the urine of 6 normal individuals.
D002356:Cartilage / Q000166:cytology / Q000378:metabolism; D003238:Connective Tissue / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis; D004340:Drug Contamination; D004815:Epidermal Growth Factor / Q000032:analysis / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006801:Humans; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology / Q000652:urine*; D011402:Pronase / Q000494:pharmacology; D011509:Proteoglycans / Q000096:biosynthesis
D006025:Glycosaminoglycans; D010455:Peptides; D011509:Proteoglycans; D004815:Epidermal Growth Factor; C009862:connective tissue-activating peptide; D004247:DNA; D011402:Pronase