title: Alterations in glycosylation of plasma membrane proteins during myogenesis.
abstract: Highly purified plasma membranes were obtained from cells of the L6 line at three characteristic stages of myogenesis: Actively proliferating cells; post-mitotic, confluent myoblasts which have already aligned; and fused myotubes. Differential glycosylation of the plasma membrane proteins of these cells was detected by staining polyacrylamide gels of the separated components with three lectins of different specificity: Concanavalin A (conA), wheat germ agglutinin (WGA) and phytohemagglutinin (PHA) Els. Four kinds of developmentally regulated changes could be identified. 1. Those which took place only at confluency (160, 150, 90, 85, 60, 43 and 40 kD for conA binding, 190 kD for WGA binding, 190 and 110 kD for PHA Els binding. 2. Those which took place only at fusion (135, 51.5 and 38 kD for conA, 160 and 150 kD for WGA and 150 kD for PHA Els binding). 3. Those where the phenomena initiated at confluency continue during fusion (66.5 and 32 kD for conA and 120 kD for PHA binding). 4. Those where opposite changes take place at confluency and at fusion (48 kD for conA, 180, 98 and 85 kD for PHA binding). These results suggest that most developmentally regulated changes in glycosylation take place during the first cell-cell contact step of myogenesis. Metabolic labelling experiments showed that, on the contrary, only few alterations in the accumulation of plasma membrane proteins take place prior to the main burst of fusion.
mesh_terms: D000818:Animals; D002455:Cell Division; D002459:Cell Fusion; D002460:Cell Line; D006023:Glycoproteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D009132:Muscles / Q000166:cytology*; D010835:Phytohemagglutinins; D011952:Receptors, Concanavalin A / Q000378:metabolism; D011975:Receptors, Mitogen / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D008565:Membrane Proteins; D010835:Phytohemagglutinins; D011952:Receptors, Concanavalin A; D011975:Receptors, Mitogen; C027503:wheat germ agglutinin receptor