Lipid bilayer dynamics and rhodopsin-lipid interactions: new approach using high-resolution solid-state 13C NMR.
High-resolution, solid-state 13C NMR spectra have been obtained for unsonicated multilamellar dispersions of 1,2-dilauryl-sn-glycero-3-phosphocholine (DLPC), recombinant membranes containing DLPC and rhodopsin, and native retinal rod disk membranes. The roles of 1H dipolar decoupling, 1H-13C cross-polarization, and magic-angle sample spinning have been investigated. Rotating-frame 13C relaxation times have been measured and are discussed in terms of lipid bilayer dynamics and rhodopsin-lipid interactions.
D005231:Fatty Acids, Unsaturated; D008051:Lipid Bilayers; D009682:Magnetic Resonance Spectroscopy; D010743:Phospholipids; D012168:Retinal Pigments; D012243:Rhodopsin; D013329:Structure-Activity Relationship
D005231:Fatty Acids, Unsaturated; D008051:Lipid Bilayers; D010743:Phospholipids; D012168:Retinal Pigments; D012243:Rhodopsin