title: Modulation of tumour colony growth by irradiated accessory cells.
abstract: The ability of human tumour cells to form colonies in soft agar is enhanced by the presence of autologous phagocytic/adherent cells. We investigated the effect of irradiation on the ability of the adherent cells to support human tumour colony formation. Relatively low doses of irradiation significantly increased the growth enhancing ability of adherent cells in 17/19 cases. The possibility that the enhancement was mediated by inactivation of radiosensitive contaminating lymphocytes was explored. Depletion of T lymphocytes from unirradiated adherent cells by a monoclonal antibody and complement resulted in little overall change in tumour colony growth. However, elimination of only the suppressor subset (OKT8+) of T lymphocytes resulted in increased colony growth relative to control values obtained with unirradiated adherent cells. In contrast, depletion of T lymphocytes from irradiated adherent cells by a pan T monoclonal antibody and complement decreased colony formation. Thus, the ability of irradiated macrophages to enhance tumour colony growth appeared to be mediated by a T lymphocyte. The effect of irradiation on isolated populations of macrophages and T lymphocytes was also examined. The enhanced ability of irradiated adherent cells to support tumor colony growth appeared to have been due to treatment of T lymphocytes alone. The results indicate that both adherent macrophages and lymphocytes may influence the growth of clonogenic human tumour cells.
mesh_terms: D000911:Antibodies, Monoclonal; D002448:Cell Adhesion / Q000528:radiation effects; D002478:Cells, Cultured; D003165:Complement System Proteins; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008212:Lymphocyte Depletion; D008264:Macrophages / Q000502:physiology / Q000528:radiation effects; D014411:Neoplastic Stem Cells / Q000502:physiology*; D013234:Stem Cells / Q000502:physiology*; D013601:T-Lymphocytes / Q000145:classification / Q000502:physiology
chemical_list: D000911:Antibodies, Monoclonal; D003165:Complement System Proteins