title: Polymorphonuclear leukocyte-mediated cytolysis induced by animal lectin.
abstract: Nine animal lectins, i.e., Sarcophaga peregrina agglutinin, Balanus roseus agglutinin, Aplysia kurodai agglutinin, Balanus balanoides agglutinin, Tetraclita squamosa japonica agglutinin, Misgurnus anguillicaudatus lectin, Asterina pectinifera agglutinin, Helix aspersa agglutinin and Helix pomatia agglutinin, were tested for induction of cytolysis mediated by polymorphonuclear leukocytes. Among them, S. peregrina agglutinin and B. roseus agglutinin lysed murine target cells in co-operation with polymorphonuclear leukocytes (PMNs) from the peritoneal cavity of mice. PMNs can lyse various tumor cells in the presence of S. peregrina agglutinin, although normal spleen cells were also lysed. This lectin-dependent cytolysis by PMNs was inhibited by galactose, a sugar which is specifically recognized by S. peregrina agglutinin. S. peregrina and B. roseus agglutinins were inhibitory to in vivo development of MM46 tumor cells. These results suggest that PMNs can lyse various target cells in the presence of appropriate animal lectins and that some animal lectins participate in tumor rejection.
mesh_terms: D000116:Acetylgalactosamine / Q000494:pharmacology; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D005690:Galactose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D037102:Lectins / Q000494:pharmacology*; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology
chemical_list: D037102:Lectins; D005947:Glucose; D000116:Acetylgalactosamine; D005690:Galactose