Rat liver glutathione S-transferases. A study of the structure of the basic YbYb-containing enzymes.
A purification scheme was devised that resulted in the resolution of a number of basic glutathione S-transferases from rat liver, three of which contained two subunits of molecular mass 23500 Da (i.e. Yb monomers). These were identified as transferases D, C and A by their elution positions from CM-cellulose and their specific activities towards a variety of substrates. Hybridization, immunotitration and peptide 'mapping' experiments demonstrated that transferases D, C and A comprise Yb2Yb2, Yb1Yb2 and Yb1Yb1 subunits.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004590:Electrophoresis, Paper; D005982:Glutathione Transferase / Q000302:isolation & purification / Q000378:metabolism; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D010446:Peptide Fragments / Q000032:analysis; D051381:Rats; D013379:Substrate Specificity
D000596:Amino Acids; D007527:Isoenzymes; D046911:Macromolecular Substances; D010446:Peptide Fragments; D005982:Glutathione Transferase