Purification of human alpha 1 antiprotease-pancreatic elastase complex. Interaction with homologous elastin.
A human alpha 1-antiprotease (alpha 1.AP)-human pancreatic elastase ( HPE11 ) complex was isolated from plasma, or prepared from commercial alpha 1.AP. The complex was identified and isolated by affinity chromatography, using Concanavalin A or IgG anti-elastase as ligands, in association with Sephacryl gel filtration. The alpha 1.AP- HPE11 complex was shown to bind with aorta and with purified aortic elastin. In both cases, the complex exhibited elastinolytic activity.
D001011:Aorta; D002846:Chromatography, Affinity; D004549:Elastin; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008970:Molecular Weight; D010196:Pancreatic Elastase / Q000302:isolation & purification* / Q000378:metabolism; D011485:Protein Binding; D000515:alpha 1-Antitrypsin / Q000302:isolation & purification*
D008024:Ligands; D000515:alpha 1-Antitrypsin; D004549:Elastin; D010196:Pancreatic Elastase