Characterization of Gardnerella vaginalis by gas chromatography.
The principal objective of this study was the characterization of Gardnerella vaginalis by gas chromatography. Thirty-eight isolates and the type strain, ATCC 14018, of G. vaginalis were studied. Hexadecanoic (16:0), octadecenoic (18:1) and octadecanoic (18:0) were the major fatty acids detected. Only insignificant differences between the various isolates could be found. The gas chromatographic analysis of G. vaginalis revealed a characteristic pattern. Gas chromatography in combination with selective growth conditions provides a method for rapid and exact identification.
D000362:Agar; D000818:Animals; D001769:Blood; D002849:Chromatography, Gas / Q000379:methods; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D006194:Gardnerella vaginalis / Q000032:analysis* / Q000254:growth & development / Q000302:isolation & purification; D006190:Haemophilus / Q000032:analysis*; D006192:Haemophilus Infections / Q000382:microbiology; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D012756:Sheep; D014627:Vaginitis / Q000382:microbiology
D005227:Fatty Acids; D000362:Agar