Effect of passively administered isologous anti-idiotypes directed against anti-carrier (ovalbumin) antibodies on the anti-hapten IgE and IgG antibody responses in BALB/c mice.
Recently we have shown that active immunization of syngenic animals with anti-ovalbumin antibodies evokes an anti-idiotypic (aId) response, which in consequence leads to suppression of the anti-hapten (benzylpenicilloyl, BPO; dinitrophenyl, DNP) IgE and IgG formation subsequently attempted by immunization with low doses of hapten-OVA conjugates. In this work we describe attempts to suppress a primary or an already established anti-hapten IgE response by passive administration of (anti-carrier) anti-idiotypes to BALB/c mice. Our results show that ongoing anti-BPO or anti-DNP IgE responses can be depressed by injection of (anti-ovalbumin) aId, provided mice were previously immunized with conjugates of the haptens with the ovalbumin (OVA) carrier. The same animals suppressed for IgE also produce less anti-hapten and anti-carrier IgG antibodies but only after 5-6 weeks following the aId injection. The primary IgE response could be blocked by treating mice with (anti-OVA) aId and antigen at the same time.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D005260:Female; D006241:Haptens / Q000276:immunology*; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007165:Immunosuppression Therapy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology*; D013997:Time Factors
D000888:Antibodies, Anti-Idiotypic; D006241:Haptens; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D007073:Immunoglobulin E; D010047:Ovalbumin