Imitation of contact inhibition by substrate-bound plasma membrane glycoproteins and lectins in serum-free hormone-supplemented cultures of GH3 cells.
N-Acetylneuraminic acid-bearing glycoproteins and lectins isolated from the plasma membrane of GH3 cells are coupled covalently to glass dishes and are used as substrates for cell culture. Under serum-free, hormone-supplemented culture conditions these coupled molecules inhibit the growth rate of GH3 cells. This inhibition is non-toxic and is concentration-and time-dependent. For an inhibition of 50% 2 X 10(6) coupled molecules contacting one cell are needed. The data suggest that specific plasma membrane-located glycoproteins and lectins are involved in the 'density-dependent growth regulation'.
D000818:Animals; D002450:Cell Communication; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006023:Glycoproteins / Q000494:pharmacology*; D037102:Lectins / Q000494:pharmacology*; D008358:Mannose / Q000378:metabolism; D008565:Membrane Proteins / Q000494:pharmacology*; D010911:Pituitary Neoplasms; D051381:Rats; D012794:Sialic Acids / Q000032:analysis; D013997:Time Factors
D006023:Glycoproteins; D037102:Lectins; D008565:Membrane Proteins; D012794:Sialic Acids; D008358:Mannose