The mouse T cell receptor: comparison of MHC-restricted receptors on two T cell hybridomas.
The receptors for antigen plus a major histocompatibility complex (MHC) gene product on a T cell hybridoma specific for ovalbumin plus a Class II MHC product were compared with those on another T cell hybridoma, specific for a Class I MHC product. In each case receptor material was identified by a clone-specific monoclonal antibody. The two receptors proved to have very similar gross structures, being 70-85 kd proteins, and reducing to an acidic alpha-chain and a slightly basic beta-chain, each 40-43 kd. The charge of both the acidic and basic polypeptides varied between the two receptors studied, showing that variable amino acid sequences occur in both chains.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D006825:Hybridomas / Q000276:immunology*; D007130:Immunoglobulin Idiotypes; D007525:Isoelectric Focusing; D046911:Macromolecular Substances; D008285:Major Histocompatibility Complex; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell