Anaerobic biodegradation of phenolic compounds in digested sludge.
We examined the anaerobic degradation of phenol and the ortho, meta, and para isomers of chlorophenol, methoxyphenol, methylphenol (cresol), and nitrophenol in anaerobic sewage sludge diluted to 10% in a mineral salts medium. Of the 12 monosubstituted phenols studied, only p-chlorophenol and o-cresol were not significantly degraded during an 8-week incubation period. The phenol compounds degraded and the time required for complete substrate disappearance (in weeks) were: phenol (2), o-chlorophenol (3), m-chlorophenol (7), o-methoxyphenol (2), m- and p-methoxyphenol (1), m-cresol (7), p-cresol (3), and o-, m-, and p-nitrophenol (1). Complete mineralization of phenol, o-chlorophenol, m-cresol, p-cresol, o-nitrophenol, p-nitrophenol, and o-, m-, and p-methoxyphenol was observed. In general, the presence of Cl and NO2 groups on phenols inhibited methane production. Elimination or transformation of these substituents was accompanied by increased methane production, o-Chlorophenol was metabolized to phenol, which indicated that dechlorination was the initial degradation step. The methoxyphenols were transformed to the corresponding dihydroxybenzene compounds, which were subsequently mineralized.
D000693:Anaerobiosis; D000840:Anisoles / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002733:Chlorophenols / Q000378:metabolism; D003408:Cresols / Q000378:metabolism; D009596:Nitrophenols / Q000378:metabolism; D010636:Phenols / Q000378:metabolism*; D012722:Sewage; D013237:Stereoisomerism
D000840:Anisoles; D002733:Chlorophenols; D003408:Cresols; D009596:Nitrophenols; D010636:Phenols; D012722:Sewage