Polyploid mesothelial cells in pleural fluid.
The Feulgen-DNA content of the mesothelial cells in non-malignant pleural fluids was cytophotometrically examined on 21 autopsy cases, in 13 of which (62%) polyploid mesothelial cells with greater than tetraploid nuclear DNA content were found. In 7 the polyploid cells appeared at the rate of 6 to 14%, 6 of which had suffered from uremia or congestive cardiac failure. The appearance rate was closely related to such pleural pathologic changes as prominent edema, diffuse hemorrhage, and fibrin precipitation. The occurrence of these polyploid cells was considered to be the result of mitotic abnormalities in the affected pleural area. Since polyploid mesothelial cells are not rare in certain non-malignant pleural fluids, a trial to establish the diagnosis of malignancy based on DNA cytophotometry alone is not recommended.
D000293:Adolescent; D000328:Adult; D000368:Aged; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D002648:Child; D004396:Coloring Agents; D004247:DNA / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D010996:Pleural Effusion / Q000166:cytology*; D011123:Polyploidy; D012394:Rosaniline Dyes
D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D004247:DNA