title: Absence of effect of danazol on estrogen-induced luteinizing hormone release from rat hypothalamo-pituitary axis in vitro.
abstract: The effects of danazol on luteinizing hormone (LH) release by estradiol (E2) were examined in a sequential double chamber superfusion system by superfusing the mediobasal hypothalamus (MBH) and pituitary excised from normal female rats in diestrus. Administration of 3x10(-13) mole E2 caused a significant (p less than 0.05) increase of LH (80-220% above the pre-injection level) from the pituitary in series with MBH superfused with Medium 199 alone or Medium 199 containing 10(-4)M danazol. These data indicate that acute administration of danazol does not affect estrogen-induced LH release in rats.
mesh_terms: D000818:Animals; D003613:Danazol / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology* / Q000502:physiology; D005260:Female; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000378:metabolism*; D011245:Pregnadienes / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D011245:Pregnadienes; D004958:Estradiol; D007986:Luteinizing Hormone; D003613:Danazol