The effect of radiation on T cell modulation of Ig synthesis by human B cells in vitro.
PWM-induced in vitro Ig synthesis was measured in cell cultures containing B lymphocytes from healthy controls and T lymphocytes collected from breast cancer patients before and after radiotherapy, respectively. The amounts of IgG and IgM did not differ between these two types of cultures. Lymphoid cell suspensions containing 3-5% monocytes were irradiated with 13 Gy and co-cultured with non-irradiated B lymphocytes. The PWM induced production of IgG and IgM in these cultures was decreased as compared to non-irradiated controls.
D001402:B-Lymphocytes / Q000276:immunology*; D001943:Breast Neoplasms / Q000532:radiotherapy; D002277:Carcinoma / Q000532:radiotherapy; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D011043:Pokeweed Mitogens / Q000276:immunology; D013601:T-Lymphocytes / Q000528:radiation effects*
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011043:Pokeweed Mitogens