Normal proliferation rate of galactocerebroside positive oligodendrocytes in brain cell cultures of the hypomyelinated mouse mutant jimpy.
Proliferation of oligodendrocytes from the jimpy (jp) hypomyelinated mouse mutant was studied in dissociated brain cell cultures. This was done by combining anti-galactocerebroside (GC) immunostaining (for identifying oligodendrocytes) with [3H]thymidine autoradiography (for identifying proliferating cells). Previously we showed that the expression of GC in culture by jp oligodendrocytes is not altered by the jp mutation. Present results show that in 7-, 14- and 21-day-old jp cultures oligodendrocytes proliferate at a rate similar to that of normal GC+ oligodendrocytes. This indicates that, in jp brain cell cultures, oligodendrocytes which are not affected by mutation in their capability to express GC are also unaffected with regard to their proliferation rate.
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000502:physiology*; D002455:Cell Division; D002478:Cells, Cultured; D002554:Cerebrosides / Q000378:metabolism*; D004261:DNA Replication; D005699:Galactosylceramides / Q000378:metabolism*; D051379:Mice; D008816:Mice, Jimpy; D009186:Myelin Sheath / Q000502:physiology*; D009457:Neuroglia / Q000502:physiology*; D009836:Oligodendroglia / Q000502:physiology*; D013045:Species Specificity; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D002554:Cerebrosides; D005699:Galactosylceramides; D014316:Tritium; D013936:Thymidine