[Infectiousness of phage T4 frozen to -196 degrees in the presence of cryoprotectors].
Polyethylene oxide with a molecular weight of 400 at a concentration of 5 to 15% and dimethyl sulfoxide at a concentration of 5% produce the protecting action when T4 phage is being frozen. The survival rate of the phage is lower when dimethyl sulfoxide at a concentration of 15% and glycerol and sucrose at a concentration from 5 to 15% are added to samples to be frozen as compared with samples frozen in the absence of the cryoprotectors.
D003451:Cryoprotective Agents / Q000494:pharmacology*; D005615:Freezing; D013604:T-Phages / Q000187:drug effects / Q000472:pathogenicity*; D013696:Temperature
D003451:Cryoprotective Agents
Infektsionnost' faga T4, zamorozhennogo do -196 gradusov pod zashchitoĭ krioprotektorov.