An improved plaque assay for viruses infecting poikilothermic cells; use of ultralow-gelling-temperature agarose.
The conventional plaque assay for viruses infecting poikilothermic cells is difficult because the cells are easily damaged at temperatures needed to keep the agarose overlay from solidifying prematurely. A modification of the assay in which these problems were solved by use of a new type of agarose (SeaPrep 15/45) that remains liquid above 15 degrees C, was developed. Use of this agarose made it easy to obtain smooth agar overlays without the risk of thermal damage to the cells.
D000818:Animals; D002478:Cells, Cultured; D011134:Polysaccharides; D012685:Sepharose; D013696:Temperature; D010948:Viral Plaque Assay / Q000379:methods*
D011134:Polysaccharides; D012685:Sepharose