Analysis of nondominant idiotypes expressed during alloimmune responses.
The antibody response of Lewis rats (RT1.A) to class I MHC antigens of the Brown Norway rat (RT1.An) was studied. Diversity of the serum alloimmune response was analyzed using syngeneic anti-idiotype raised against monoclonal antibodies of the same specificity. Cross-reactive idiotypes were detected on approximately one in one thousand Lewis anti-RT1.An serum antibodies, at concentrations ranging from 20 to 600 ng/ml. The kinetics of idiotype expression coincided with that of total anti-BN antibody production, suggesting that both were regulated by the same mechanism. To determine whether humoral anti-idiotype was involved in such regulation, sera from these animals were screened for anti-idiotype content. Using an RIA sensitive to 20 ng/ml, no humoral anti-idiotype could be detected during any phase of the alloimmune response.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D000918:Antibody Specificity; D000961:Antilymphocyte Serum / Q000235:genetics / Q000494:pharmacology; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D001789:Blood Group Antigens / Q000276:immunology; D003429:Cross Reactions; D005799:Genes, Dominant; D007130:Immunoglobulin Idiotypes / Q000235:genetics / Q000276:immunology*; D007518:Isoantibodies / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew
D000888:Antibodies, Anti-Idiotypic; D000961:Antilymphocyte Serum; D001789:Blood Group Antigens; D007130:Immunoglobulin Idiotypes; D007518:Isoantibodies