The nature of the stable noncovalent dimers of band 3 protein from erythrocyte membranes in solutions of Triton X-100.
Stable noncovalent dimers of band 3 protein from human erythrocyte membranes, in which state the protein is thought to exist after solubilization by the nonionic detergent Triton X-100, do not occur when purified batches of the detergent are used. Instead, the protein is in a monomer/dimer/tetramer association equilibrium. The stable dimers do appear, however, when the detergent has been 'aged'. They thus seem to be artifacts.
D001457:Anion Exchange Protein 1, Erythrocyte / Q000302:isolation & purification*; D003902:Detergents; D004910:Erythrocyte Membrane / Q000032:analysis*; D006801:Humans; D046911:Macromolecular Substances; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols; D014461:Ultracentrifugation
D001457:Anion Exchange Protein 1, Erythrocyte; D003902:Detergents; D046911:Macromolecular Substances; D011092:Polyethylene Glycols; D017830:Octoxynol