[Effect of acetylcholine, cholinomimetics, cholinolytics and dipiroxim on the chemiluminescence of a model system of peroxidation].
Use was made of a model system initiating lipid peroxidation, that contained phosphate buffer, H2O2 and eosine. Chemiluminescence of the system decreased after addition to rat brain synaptosomes, thereby indicating the presence of antioxidants in the animals' brain. After addition of brain synaptosomes of rats poisoned with high lethal doses of armine (0.4--0.5 mg/kg) a prooxidant effect was observed. It was manifested in the elimination of the effect of natural antioxidants contained by synaptosomes of normal rats. Addition of acetylcholine, armine, DFP and oxazyl at concentrations of 1.10(-4) 1.10(-3) M to the model system containing rat brain synaptosomes entailed a considerable intensification of chemiluminescence. Atropine and scopolamine did not produce any substantial effect on chemiluminescence, whereas dipiroxim exerted an overt antioxidant effect. The conclusion is made about the possibility of activating the system of lipid peroxidation as one of the components of the toxic action of anticholinesterase agents.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001138:Armin / Q000506:poisoning; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism*; D008163:Luminescent Measurements; D010084:Oxidation-Reduction / Q000187:drug effects; D010091:Oximes / Q000494:pharmacology*; D010276:Parasympatholytics / Q000494:pharmacology*; D010277:Parasympathomimetics / Q000494:pharmacology; D051381:Rats; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism; D014289:Trimedoxime / Q000494:pharmacology*
D008054:Lipid Peroxides; D010091:Oximes; D010276:Parasympatholytics; D010277:Parasympathomimetics; D014289:Trimedoxime; D001138:Armin; D000109:Acetylcholine
Vliianie atsetilkholina, nekotorykh kholinomimetikov, kholinolitikov i dipiroksima na khemiliuminestsentsiiu model'noĭ sistemy perekisnogo okisleniia.