Induction by chloroform of two forms of ornithine decarboxylase in rat liver. Half-life of isozymes.
Ornithine decarboxylase (ODC) in rat liver was separated into two species by DEAE-Sepharose CL-6B column chromatography. The activity of both species of ODC was increased at least 20-fold by chloroform treatment of the rats. The major species, Peak A, contained 65% of the ODC activity and possessed a half-life of 11 min. The second species, Peak B, accounted for 35% of the activity and possessed a half-life of 50 min. The long-lived species of ODC activity, induced in rat liver by chloroform, has not been reported previously and might be related to the prolonged induction of ODC activity by chloroform and to tumor promotion and growth.
D000818:Animals; D002262:Carboxy-Lyases / Q000235:genetics*; D002725:Chloroform / Q000494:pharmacology*; D003513:Cycloheximide / Q000494:pharmacology; D004790:Enzyme Induction; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D009955:Ornithine Decarboxylase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344
D007527:Isoenzymes; D002725:Chloroform; D003513:Cycloheximide; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase