[Detection and determination of protein-bound formaldehyde. II. Improved recovery of formaldehyde by reduction with sodium cyanoborohydride (NaCNBH3)].
When using NaCNBH3 as a reductant, it is possible to recover 70% of added formaldehyde in foods--in this skim milk powder. Formaldehyde is attached to the epsilon-Aminogroups of lysine and is detected after reduction in form of N epsilon-methyllysine in an automated amino acid analyzer system. After addition of N alpha-acetyllysine the reduction is carried out by incubating the skim milk powder at 70 degrees C for 1 h. The best combination of pH, concentration of reductant, time and temperature of incubation is described.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001894:Borohydrides; D002417:Cattle; D005557:Formaldehyde / Q000032:analysis*; D008892:Milk / Q000032:analysis; D010084:Oxidation-Reduction; D011485:Protein Binding; D013696:Temperature; D013997:Time Factors
D000596:Amino Acids; D001894:Borohydrides; D005557:Formaldehyde; C009282:sodium cyanoborohydride
Nachweis und Bestimmung von Protein-gebundenem Formaldehyd. II. Verbesserte Wiederfindung von Formaldehyd durch Reduktion mit Natriumcyanoborhydrid (NaCNBH3).