title: Cultured beating myocytes from neonatal rat heart as a model for the study of muscarinic cholinergic receptors.
abstract: Neonatal rat heart myocytes were isolated by digestion of the heart tissue with trypsin. After separation the cells were transferred to plastic cluster dishes where they formed a monolayer culture. After 72 hours in culture the myocytes showed a synchronous contraction and renovation of their membrane structures. Studies of muscarinic cholinergic receptors were carried out using (3H)-Quinuclidinyl benzilate directly on cells attached to the bottom of the dish. The presence of a specific muscarinic receptor on cultured beating myocytes was confirmed.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004837:Epinephrine / Q000494:pharmacology; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D011433:Propranolol / Q000494:pharmacology; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000378:metabolism*
chemical_list: D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate; D011433:Propranolol; D004837:Epinephrine