Recognition and lysis of altered-self cells by macrophages. I. Modification of target cells by 2,4,6-trinitrobenzene sulphonic acid.
Peritoneal exudate macrophages from normal, untreated or thioglycollate-elicited mice, lysed syngeneic fibroblasts and lymphoblasts modified by 2,4,6-trinitrobenzene sulphonic acid (TNBS) in vitro. Optimal lysis of the hapten-modified cells by elicited macrophages was usually seen after 18 hr of co-cultivation at E:T ratios of 10:1-30:1. Cytotoxicity was expressed by macrophages depleted of T cells, and was not potentiated by LPS. Allogeneic TNBS-modified cells were lysed by non-immune, non-activated macrophages to the same extent as syngeneic modified targets, indicating that genetic restriction does not appear to play a role in macrophage-mediated cytolysis of TNBS-modified cells.
D000818:Animals; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D005260:Female; D005347:Fibroblasts / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D009578:Nitrobenzenes / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology*
D008070:Lipopolysaccharides; D009578:Nitrobenzenes; D014302:Trinitrobenzenesulfonic Acid