[Localization of the LDHA-GST3-ESA4 synthetic group on human chromosome 11. Analyses of the classic man-rodent hybrids and of a new type (not adhering to the wall)].
Analysis of different human tissues showed that glutathione S-transferase (GST) of fibroblasts and leucocytes is GST3, an enzyme found in liver extracts by Board (1981). Consequently, the GST localized on human chromosome 11 by Silberstein and Shows (1981, 1982) is GST3. Analysis of tissue extracts showed a new GST band, very intense in muscle extracts and very weak or absent in other tissues, and called GSTM. Analysis of man-rodent hydrids showed synteny between LDHA, GST3, ESA4 and localization of this synteny group on chromosome 11. Analysis of discordant percentages and discordant types between markers favored the following assignments: LDHA on 11p12, GST3 on 11q13 leads to 11qter, ESA on 11q13 leads to 11q22. The present study suggests assignment of GST3 to 11q13 leads to 11q22. Different types of man-rodent hybrids are useful for human gene mapping. A new type hybrid, as used here (nonadhering to glass or plastic surfaces), appears useful because of rapid proliferation and growth in suspension, the latter feature facilitating culture and harvesting.
D000818:Animals; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D002460:Cell Line; D002906:Chromosomes, Human, 6-12 and X / Q000648:ultrastructure*; D006224:Cricetinae; D004586:Electrophoresis; D005813:Genes, Synthetic; D005819:Genetic Markers; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology; D007527:Isoenzymes; D007621:Karyotyping; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D051379:Mice
D005819:Genetic Markers; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D005982:Glutathione Transferase; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C469538:ESA4 protein, human
Localisation du groupe synténique LDHA-GST3-ESA4 sur le chromosome 11 chez l'homme. Analyses des hybrides homme-rongeur classiques et d'un type nouveau (non adhérents à la paroi).