Use of benzyldimethyl-n-hexadecylammonium chloride ("16-BAC"), a cationic detergent, in an acidic polyacrylamide gel electrophoresis system to detect base labile protein methylation in intact cells.
A discontinuous polyacrylamide gel system operating at pH 4.0-1.5 which resolves proteins bearing base labile groups extracted from intact cells is described. It uses potassium phosphate buffer in the running and stacking gel and glycine as the trailing ion component. Proteins are solubilized with urea and benzyldimethyl-n-hexadecylammonium chloride, a cationic detergent. The utility of the system is illustrated by fluorographs of the pattern of protein methylation in blood platelets and the HL60 promyelocyte cell line.
D001792:Blood Platelets / Q000378:metabolism; D002460:Cell Line; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D005233:Fatty Alcohols; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008745:Methylation; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents
D003902:Detergents; D005233:Fatty Alcohols; D011506:Proteins; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; C012817:cetalkonium chloride