Fluorographic detection of tritium-labelled proteins in immunoelectropherograms with the water-soluble fluor, sodium salicylate.
A method is described for detecting by fluorography tritium-labelled proteins in immunoelectropherograms performed on agarose gels. The technique is based on the impregnation of immunoplates with sodium salicylate immediately after electrophoresis without the formation of a mixed agarose--polyacrylamide gel prior to processing the immunoelectropherograms for fluorography. Sodium salicylate, an inexpensive and water-soluble compound, has been found to serve as a satisfactory fluor for enhanced detection of radioactivity in agarose gels.
D001345:Autoradiography; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D007122:Immunoelectrophoresis / Q000379:methods*; D012459:Salicylates; D014316:Tritium / Q000032:analysis*
D012459:Salicylates; D014316:Tritium