Zinc binding in cow's milk and human milk.
In both cow's milk and human milk, zinc was associated with proteins of high molecular weight (greater than 100 000), as judged by analysis with Sephadex G-75. Precipitation of the casein at pH 4.6 and filtration of the resultant acid whey on Sephadex G-25 led, however, to the recovery of about 90% of the zinc as a compound of low molecular weight, which was tentatively identified as zinc citrate. Over 95% of the zinc of cow's milk was sedimented with the casein micelles on ultracentrifugation. Filtration of these micellar caseins on Sephadex G-150 gave two peaks containing zinc, which corresponded to aggregates of alpha-casein-kappa-casein and of alpha-casein-beta-casein. Ultracentrifugation of human milk sedimented only approx. 40% of total zinc. Analysis of sediment and supernatant on Sephadex G-150, however, indicated that about 85% of the zinc was associated with a protein complex of molecular weight greater than 150 000. The major protein of this complex was identified as lactoferrin. A minor zinc-binding component of average molecular weight 30 000 was also observed in the supernatant. The results indicated that zinc is bound to different macromolecules in cow's and human milk. This may be a factor affecting the bioavailability to the human infant of zinc from the two milks, and it is suggested that in human milk lactoferrin may be involved in the uptake of zinc.
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002417:Cattle; D002951:Citrates / Q000378:metabolism; D019343:Citric Acid; D003611:Dairy Products / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007225:Infant Food; D007785:Lactose / Q000378:metabolism; D008892:Milk / Q000378:metabolism*; D008895:Milk, Human / Q000378:metabolism*; D008970:Molecular Weight; D014461:Ultracentrifugation; D015032:Zinc / Q000378:metabolism*
D002352:Carrier Proteins; D002951:Citrates; C017800:zinc-binding protein; D019343:Citric Acid; D007785:Lactose; D015032:Zinc