Presence of immunoreactive corticotropin-releasing factor in human cerebrospinal fluid.
Immunoreactive corticotropin-releasing factor (I-CRF) was measured by radioimmunoassay and immunoaffinity chromatography in human hypothalamus and cerebrospinal fluid (CSF). Dilution curves of I-CRF in the hypothalamus and CSF were parallel to that of synthetic ovine CRF standard. I-CRF content in the hypothalamus was 643 and 281 fmol eq, respectively. I-CRF concentration in CSF was 7.4 +/- 1.1 fmol eq/ml. Sephadex G-75 column chromatography showed the main peak eluted at the position of synthetic CRF.
D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003346:Corticotropin-Releasing Hormone / Q000032:analysis / Q000134:cerebrospinal fluid*; D006801:Humans; D007031:Hypothalamus / Q000032:analysis*; D011863:Radioimmunoassay
D003346:Corticotropin-Releasing Hormone