title: The HPLC analysis of thiamine in milk chocolate.
abstract: An HPLC method for the analysis of thiamin in milk chocolate and various chocolate products is described. Samples are extracted with H2SO4, treated with enzymes and interferences removed with a small cleanup column. The separation is effected on a monolayer reversed phase column with a post column thiochrome reaction and fluorescence detection. The method is accurate and precise.
mesh_terms: D002099:Cacao / Q000032:analysis*; D002182:Candy / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D013831:Thiamine / Q000032:analysis*
chemical_list: D013831:Thiamine