Phosphoenolpyruvate-dependent phosphorylation of alpha-methylglucoside in Streptococcus sanguis ATCC 10556.
Spontaneous mutants defective in some undefined membrane components of the phosphoenolpyruvate:glucose phosphotransferase system were isolated by plating cells of Streptococcus sanguis ATCC 10556 onto an agar containing lactose and 10 mM 2-deoxyglucose. Toluenized cells of these mutants were defective in their ability to catalyse the phosphoenolpyruvate-dependent phosphorylation of 2-deoxyglucose but were still able to phosphorylate alpha-methylglucoside. The phosphorylation of alpha-methylglucoside was essentially dependent on phosphoenolpyruvate and required the presence of both soluble and membrane components. It was concluded that S. sanguis possessed two different phosphoenolpyruvate:glucose phosphotransferase systems.
D003847:Deoxyglucose / Q000378:metabolism; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D009154:Mutation; D010728:Phosphoenolpyruvate / Q000494:pharmacology; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D010766:Phosphorylation; D013298:Streptococcus sanguis / Q000201:enzymology* / Q000378:metabolism
D008757:Methylglucosides; D008759:Methylglycosides; C027020:methylglucoside; D010728:Phosphoenolpyruvate; D003847:Deoxyglucose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System