title: Kinetics of the antigen:antibody reaction. Light scattering and particle size measurement using photon correlation spectroscopy.
abstract: Many techniques for the estimation of specific proteins measure light scattered by, or the turbidity of, suspensions of antigen:antibody aggregates formed in fluid-phase reactions. Although the shapes of reaction curves obtained are well documented, interpretation of the data in relation to the laws of light scattering and the mechanism of the antigen:antibody reaction are poorly described in the literature. Using photon correlation spectroscopy it has been shown that antigen:antibody complexes continue to grow in size even when no further increases in scatter intensity or turbidity are apparent.
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis*; D006801:Humans; D007106:Immune Sera; D007074:Immunoglobulin G; D007700:Kinetics; D012756:Sheep / Q000276:immunology; D013053:Spectrophotometry / Q000379:methods
chemical_list: D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007074:Immunoglobulin G