Phenol-soluble nonhistone chromatin proteins in chronic lymphocytic leukemia.
The altered gene expression seen in cancer could relate to differences in nonhistone chromatin proteins between normal and malignant tumor cells. Phenol-soluble nonhistone chromatin proteins were isolated from human normal and leukemic (chronic lymphocytic leukemia) B-cells, as well as long-term cultured human B-lymphocyte cell lines. High-resolution two-dimensional electrophoretic maps identified a group of three nuclear proteins with a molecular weight of 45,000 to 50,000 and an isoelectric range of 4.5 to 4.7, which were associated only with the human leukemic B-cells. Leukemic B-cells and cultured B-cell lines also expressed a variant form of nuclear actin and tubulin.
D001402:B-Lymphocytes / Q000032:analysis; D002460:Cell Line; D002478:Cells, Cultured; D002868:Chromosomal Proteins, Non-Histone / Q000097:blood / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007945:Leukemia, Lymphoid / Q000378:metabolism*; D007962:Leukocytes / Q000032:analysis*; D008970:Molecular Weight; D019800:Phenol; D010636:Phenols; D012016:Reference Values
D002868:Chromosomal Proteins, Non-Histone; D010636:Phenols; D019800:Phenol