Semi-micro methods for analysis of labile sulfide and of labile sulfide plus sulfane sulfur in unusually stable iron-sulfur proteins.
Details are provided for a reproducible procedure for determination of labile sulfide in iron-sulfur (Fe-S) proteins in the range of 1 to 3 nmol. Analyses are also presented on the most stable Fe-S protein so far reported. In this case denaturation with guanidine.HCl was used in the presence of dithiothreitol. The values obtained then also include any sulfane sulfur (S0) present.
D003901:Desulfovibrio / Q000032:analysis; D019791:Guanidine; D006146:Guanidines; D007506:Iron-Sulfur Proteins / Q000032:analysis*; D008667:Metalloproteins / Q000032:analysis*; D011489:Protein Denaturation; D013053:Spectrophotometry; D013440:Sulfides / Q000032:analysis*; D013455:Sulfur / Q000032:analysis*
D006146:Guanidines; D007506:Iron-Sulfur Proteins; D008667:Metalloproteins; D013440:Sulfides; D013455:Sulfur; D019791:Guanidine