Phorbol ester increases the level of interleukin 2 mRNA in mitogen-stimulated human lymphocytes.
TPA alone did not induce the production of IL 2 in human tonsillar lymphocytes but enhanced the PHA-induced IL 2 production by seven-fold. That the effect of TPA was due to an increase in IL 2 mRNA was demonstrated by examining the amount of IL 2 mRNA translatable in Xenopus laevis oocytes, and by Northern blotting analysis using IL 2 cDNA as a probe. In these ways, it was shown that TPA alone did not induce any significant IL 2 mRNA synthesis, but when added together with PHA it increased the level of IL 2 mRNA by at least 10-fold, as compared with that induced by PHA alone.
D000818:Animals; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D009865:Oocytes / Q000378:metabolism; D014066:Palatine Tonsil / Q000166:cytology; D010704:Phorbols / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014982:Xenopus laevis
D007376:Interleukin-2; D010704:Phorbols; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D013755:Tetradecanoylphorbol Acetate