[Isolation of cell lines transformed by highly oncogenic simian adenovirus SA7 and nononcogenic human adenovirus type 6 and their DNAs].
Methods for generation of cell lines transformed by highly oncogenic simian adenovirus SA7, nononcogenic human adenovirus type 6 and their DNAs are described. WAG rat kidney cells were used for transformation. To produce 1 focus of transformation, 1.7 X 10(6) PFU of SA7 virus is required. Intact and fragmented DNA of both viruses may be quite effectively used for transformation. For production of 1 transformation focus 1.2 microgram SA7 DNA and 1.1 microgram type 6 adenovirus DNA is required. In most cases, DNA fragmentation increases the transforming activity which has been shown to be associated with the left genome region of both viruses under study.
D000256:Adenoviridae / Q000472:pathogenicity*; D000260:Adenoviruses, Human / Q000472:pathogenicity*; D000261:Adenoviruses, Simian / Q000472:pathogenicity*; D000818:Animals; D002460:Cell Line; D002469:Cell Separation; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000382:microbiology; D002472:Cell Transformation, Viral / Q000187:drug effects; D006224:Cricetinae; D004279:DNA, Viral / Q000494:pharmacology*; D006801:Humans; D007668:Kidney; D008647:Mesocricetus; D051381:Rats; D014776:Virus Cultivation
D004279:DNA, Viral
Poluchenie liniĭ kletok, transformirovannykh vysokoonkogennym adenovirusom obez'ian SA7 i neonkogennym adenovirusom cheloveka tipa 6 i ikh DNK.