[Prothrombin--substrate for blood plasma kallikrein and factor XIIa].
A study was made of the interaction between prothrombin and enzymes: blood plasma kallikrein and factors alpha-XIIa and beta-XIIa immobilized on enzacryl-AH. Kallikrein-induced prothrombin proteolysis was accompanied by a decrease in prothrombin activity, appearance of BAME-esterase and poor clotting activity. As a result of fractionation of products on the column with DEAE-Sephadex A-50, some fractions that have thrombin amidase activity (splitting of the substrate S-2238) and high antithrombin activity were obtained. Antithrombin activity manifested in the inhibition of fibrinmonomer aggregation during fibrin formation. During incubation with prothrombin, factors alpha-XIIa and beta-XIIa also stimulated the appearance of BAME-esterase activity. None of the immobilized enzymes activated factor X.
D000180:Acrylic Resins; D000818:Animals; D002417:Cattle; D004800:Enzymes, Immobilized; D005174:Factor XII / Q000378:metabolism*; D015956:Factor XIIa; D006801:Humans; D066298:In Vitro Techniques; D007610:Kallikreins / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D011516:Prothrombin
D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D010446:Peptide Fragments; C032056:enzacryl AH; D011516:Prothrombin; D005174:Factor XII; D007610:Kallikreins; D015956:Factor XIIa
Protrombin--substrat kallikreina plazmy krovi i faktora XIIa.