[Brain proteolipids in representatives of different vertebrate classes].
The Folch-Lees proteolipid complexes of different purity (crude proteolipids and relative pure proteolipids) were isolated from vertebrate brain: mammalia (Macaca irus, Macaca rhesus and white rat), birds (Columbia livia), reptilia (Testudo horsfieldi), amphibia (Rana temporaria) and fishes (Salmo irideus). The proteolipid complexes were isolated by emulsion-centrifugation method. The content of proteolipid protein (mg/g w. w.) correlates with the level of phylogenetic development of the animals studied. It is the highest in monkey brain (10.5 and 8.6 mg/g) and the lowest in fish brain (2.2 mg/g). The yield of proteolipids from the brains of animals studied shows the same pattern. Crude proteolipids of mammalia, birds and reptiles contain 40-50% of protein and 60-50% of lipids. The content of phospholipids is about 40%. Proteolipids of amphibia and fish brain contain less protein--about 30%. In the conditions of mild purification, the protein content in mammalia, birds and reptilia makes up about 70% and lipid content--about 30-35%. The crude and purified proteolipids in all the animals studied (as compared with the original lipid extracts from which they were isolated) are enriched in acid phospholipids: phosphatidyl serine, phosphatidyl inositol and diphosphatidyl glycerol. Acid phospholipids in total lipid extract make up 10-20% of total phospholipids, in crude proteolipids 16-32 and in purified proteolipids--56-75%. There are no marked differences between fatty acid composition of phospholipids in proteolipids and in the same phospholipids isolated from total lipid extract.
D000818:Animals; D001923:Brain Chemistry; D010856:Columbidae; D005227:Fatty Acids / Q000032:analysis; D008251:Macaca; D008253:Macaca mulatta; D010743:Phospholipids / Q000032:analysis; D011510:Proteolipids / Q000032:analysis* / Q000302:isolation & purification; D011896:Rana temporaria; D051381:Rats; D012487:Salmonidae; D014426:Turtles; D014714:Vertebrates / Q000378:metabolism*
D005227:Fatty Acids; D010743:Phospholipids; D011510:Proteolipids
Proteolipidy mozga u predstaviteleĭ raznykh klassov pozvonochnykh.