title: Alloreactive T-cell clones. Ly phenotypes predict both function and specificity for major histocompatibility complex products.
abstract: We have studied the association of Ly phenotype with function and specificity for major histocompatibility complex (MHC) products by examining the properties of 21 T-cell clones derived from B10 anti-B10.D2 and B10.A anti-B10.D2 mixed lymphocyte cultures (MLC). T cells were selected after MLC solely on the basis of Ly phenotype, cloned by limiting dilution, and tested for stability of Ly phenotype, function and specificity for class I or class II MHC products. Sixteen Ly-1+2- and five Ly-1-2+ T-cell clones were tested. The clones selected for the Ly-1+2- phenotype maintained this phenotype, expressed helper but not lytic function, and recognized class II MHC products (I-Ad or I-Ed). All Ly-1-2+ clones maintained this phenotype, possessed cytolytic but not helper activity, and recognized class I MHC products (Dd and Ld). Our data therefore confirm at the clonal level the original observations of a remarkably consistent correlation between Ly markers, MHC specificity, and function. They suggest that the expression of Ly antigens on T-cell clones forms part of a genetic program for each of these specialized cells that also determines their function and MHC specificity.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000950:Antigens, Ly / Q000235:genetics*; D002999:Clone Cells; D005260:Female; D007376:Interleukin-2 / Q000096:biosynthesis; D008285:Major Histocompatibility Complex; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology* / Q000502:physiology
chemical_list: D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D007376:Interleukin-2