Preparation of high resolution chromosomes from amniotic fluid cells.
A relatively simple method of obtaining high resolution chromosomes from amniotic fluid cells is described. The elongated chromosomes are achieved by adding ethidium bromide (5 micrograms/ml) to the culture 4 1/2 hours before harvesting and the high resolution banding can be produced by usual banding procedures.
D000653:Amniotic Fluid / Q000166:cytology*; D002869:Chromosome Aberrations / Q000175:diagnosis*; D002871:Chromosome Banding / Q000379:methods*; D025063:Chromosome Disorders; D002875:Chromosomes / Q000187:drug effects; D004996:Ethidium / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*
D004996:Ethidium