The resolution of bimodal peaks in the analytical ultracentrifuge in the presence and absence of ligands.
The patterns for peak resolution in the analytical ultracentrifuge characteristic of systems associating by different mechanisms are analyzed. These are (i) that in which reequilibration is infinitely slow; (ii) that in which reequilibration is rapid, i.e., a Gilbert-type system; and (iii) that in which reequilibration is rapid, but the self-association is mediated by a ligand. Criteria for distinguishing the three are described and illustrated by results on tubulin self-association under various conditions.
D002021:Buffers; D008024:Ligands; D011108:Polymers; D014404:Tubulin / Q000032:analysis; D014461:Ultracentrifugation / Q000295:instrumentation / Q000379:methods*
D002021:Buffers; D008024:Ligands; D011108:Polymers; D014404:Tubulin