Analysis of proportion regulation in slugs of Dictyostelium discoideum using a monoclonal antibody and a FACS-IV.
A quantitative assay for estimating the proportion of prespore cells in D. discoideum slugs was established by labelling disaggregated slug cells with a prespore specific monoclonal antibody and analysing the cell population with a FACS-IV. The method is validated using a wild-type strain and its stalky mutant. "Wild-type" strains have different proportions of prespore cells and it is demonstrated that slugs of some strains have an increased percentage of prespore cells when migrated in the dark compared to the light and in the presence of EGTA. The technique is rapid and will make possible genetic analysis of proportion regulation in D. discoideum.
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D002469:Cell Separation; D004023:Dictyostelium / Q000166:cytology / Q000235:genetics*; D005453:Fluorescence
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface