Isolation by flow sorting of cytokinetic and morphological heterogeneity in late-passage cultures of human diploid fibroblasts (IMR-90).
Considerable structural, metabolic, and proliferative heterogeneity develops in populations of cultured diploid cells which have reached advanced levels of population doubling. Isolation of noncycling cells from late-passage cultures would permit more definitive investigation of the structure and behavior of individual senescent cells. In this paper, we report the viable sorting of late-passage cultures of human diploid fibroblasts (IMR-90) into two subpopulations of cells with different proliferative potentials. Sorting is based on cellular light-scattering properties and autofluorescence. Structural and behavioral features of the subpopulation exhibiting increased forward-angle light scatter are more characteristic of senescent cells than the subpopulation sorted by decreased forward-angle light scatter.
D001345:Autoradiography; D002455:Cell Division; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D005347:Fibroblasts / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D008027:Light; D008854:Microscopy, Electron; D012542:Scattering, Radiation
D004247:DNA