The effect of oxalate on gluconeogenesis by isolated chicken hepatocytes. Increased sensitivity to inhibition as a result of biotin deficiency.
Addition of varying concentrations of oxalate to isolated chicken hepatocytes reduced gluconeogenesis from lactate in a manner indicating that pyruvate carboxylase was not the rate-limiting step. With hepatocytes from biotin-deficient chicks, sensitivity to inhibition was increased, and was consistent with pyruvate carboxylase being rate-limiting. Administration of biotin to deficient chicks overnight restores sensitivity to oxalate to normal.
D000818:Animals; D001710:Biotin / Q000172:deficiency*; D002645:Chickens; D005260:Female; D005943:Gluconeogenesis / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D010070:Oxalates / Q000494:pharmacology*; D019815:Oxalic Acid
D010070:Oxalates; D001710:Biotin; D019815:Oxalic Acid