Noncompetitive enzyme immunoassay for the measurement of bronchial inhibitor in biological fluids.
An enzyme-linked immunosorbent assay (ELISA) of bronchial inhibitor using rabbit antibronchial inhibitor antibody-coated polystyrene balls as the solid-phase antibody and peroxidase-labeled antibody as the conjugate is described. A crude antibody fraction is used for coating the solid phase. The assay can be run within 8 h and gives reproducible results in the range of 6 to 60 micrograms/l of bronchial inhibitor (mean within-run coefficient of variation, 7%). It can detect bronchial inhibitor concentrations as low as 2 micrograms/l (10(-10) M) and recovery of varying amounts of bronchial inhibitor added to bronchial liquids is greater than 90%. This enzyme immunoassay appears to be a convenient way to quantify bronchial inhibitor in biological fluids such as serum, sputum, or bronchoalveolar lavage fluid.
D000918:Antibody Specificity; D001980:Bronchi / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006098:Granulocytes / Q000201:enzymology; D006801:Humans; D007962:Leukocytes / Q000201:enzymology; D008173:Lung Diseases, Obstructive / Q000097:blood*; D011480:Protease Inhibitors / Q000032:analysis*; D013183:Sputum / Q000032:analysis
D011480:Protease Inhibitors