[Effect of heterogeneous inductors on the ectoderm of the early gastrula in Rana temporaria in vitro. 5. Biochemical analysis of induction-active extracts from chick embryo retina and brain].
The water extracts from the retina and brain of 7-8-day old chick embryos were centrifuged at 20,000 g; sediments were discarded and supernatants were additionally centrifuged at 110,000 g. The inductive activity of supernatants (20,000 and 110,000 g) and sediments (110,000 g) was estimated in vitro on the Rana temporaria early gastrula ectoderm. The neutralizing activity was related exclusively to the soluble fractions of the extracts from the chick embryo retina and brain. The lens-inducing activity appeared to be characteristic of both the supernatants and the microsome fractions of these extracts. A comparative biochemical analysis of the extracts (isoelectrofocusing, electrophoresis in the presence of sodium dodecylsulfate, electroblotting) has shown that the chick embryo retina and brain are similar by the spectrum and properties of peptides. It is suggested that the similarity of the extracts inducing effect on the early gastrula ectoderm is due to the presence of the same proteins (peptides) in the retina and brain. Peptides with a positive immunohistochemical reaction to vimentin and peptides of neurofilaments were found in trace quantities in the retina and brain extracts by means of immunoelectroblotting.
D000818:Animals; D001921:Brain / Q000196:embryology*; D001923:Brain Chemistry; D002642:Chick Embryo; D004475:Ectoderm / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D005775:Gastrula / Q000187:drug effects*; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D010455:Peptides / Q000032:analysis; D011896:Rana temporaria / Q000196:embryology*; D012160:Retina / Q000032:analysis / Q000196:embryology*; D014020:Tissue Extracts / Q000032:analysis / Q000494:pharmacology*; D014461:Ultracentrifugation
D010455:Peptides; D014020:Tissue Extracts
Vliianie geterogennykh induktorov na éktodermu ranneĭ gastruly Rana temporaria in vitro. 5. Biokhimicheskiĭ analiz induktsionno-aktivnykh ékstraktov iz setchatki i golovnogo mozga zarodysheĭ kur.