Induction of B lymphocyte proliferation by monoclonal anti-Lyb 2 antibody.
Monoclonal antibody to Lyb 2, a differentiation antigen present on all B cells, has been used to study the role of Lyb 2 molecules in B cell activation. Monoclonal anti-Lyb 2 antibody (m-anti-Lyb 2) transforms resting B cells into blast cells and induces proliferation in these activated B cells. The proliferative response to anti-Lyb 2 is a property of the Lyb 5+ subset of B cells, since the antibody fails to stimulate B cells from mice expressing the CBA/N immune defect. B cells activated by anti-Lyb 2 mature into antibody-secreting cells in the added presence of T cell-replacing factors contained in the supernatants from concanavalin A-activated T cells. Thus, Lyb 2 molecules may participate in the delivery of stimulatory signals during the early phases of B lymphocyte activation.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D000921:Antibody-Producing Cells / Q000276:immunology; D000950:Antigens, Ly / Q000276:immunology*; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology*; D003208:Concanavalin A / Q000502:physiology; D005260:Female; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D008213:Lymphocyte Activation; D008222:Lymphokines; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D007140:Immunoglobulin Fab Fragments; D008222:Lymphokines; C021649:concanavalin A-induced helper factors; D003208:Concanavalin A