Identification of canine T lymphocytes by membrane receptor to peanut agglutinin: T-lymphocyte identification in dogs with lupus-like syndrome.
Canine T lymphocytes were detected, using fluorescent peanut agglutinin (PNA) as a marker. Using a fluorescent technique and cytofluorometry, 70 +/- 11% and 72.4%, respectively, of peripheral blood lymphocytes were bound to PNA. Of thymocytes, 97 +/- 4.5% were detected by fluorescent PNA, but less than 1% were detected for lymphocytes from bone marrow. The T-lymphocyte depletion and enrichment indicated that PNA was bound to lymphocytes recognized by anti-T-lymphocyte heterologous serum. A T-lymphocyte deficiency was detected among 8 dogs with a lupus-like syndrome.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002469:Cell Separation; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D037102:Lectins / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000662:veterinary*; D011971:Receptors, Immunologic / Q000032:analysis; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*; D014909:Wheat Germ Agglutinins
D037102:Lectins; D011971:Receptors, Immunologic; D014909:Wheat Germ Agglutinins