H4-isozyme of lactate dehydrogenase in a solution of sodium C chloride--6. The effect of oxalate and oxamate on the molecular weight.
Some differences between the effects of oxalate and oxamate were observed. The oxalate formed the stable tetramers and some aggregates, while the oxamate formed the mixture of dimers, tetramers, octamers and aggregates. The ratios between these molecular forms were different as the oxamate concentration was changed. In the coexistence of inhibitors and pyruvate, pyruvate may act to decrease the molecular weight and to increase the amount of aggregates. The lower molecular weight may be caused by the existence of the active complex, Ed N S1. The effect of the exchange between pyruvate and oxamate bound with enzyme complexes may be expected.
D000596:Amino Acids; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors; D046911:Macromolecular Substances; D008970:Molecular Weight; D009994:Osmolar Concentration; D010070:Oxalates; D019815:Oxalic Acid; D010072:Oxamic Acid; D011773:Pyruvates; D019289:Pyruvic Acid; D012965:Sodium Chloride
D000596:Amino Acids; D007527:Isoenzymes; D046911:Macromolecular Substances; D010070:Oxalates; D011773:Pyruvates; D012965:Sodium Chloride; D019289:Pyruvic Acid; D019815:Oxalic Acid; D007770:L-Lactate Dehydrogenase; D010072:Oxamic Acid