Chick embryo spinal cord neurons synthesize a transferrin-like myotrophic protein.
Highly enriched cultures of chick embryo spinal cord neurons synthesize and secrete a protein which is immunoprecipitable by anti-ovotransferrin. Ovotransferrin, an iron-binding glycoprotein of Mr 80 000, is also shown to stimulate in vitro myogenesis of cultured chick embryo myotubes as measured by saturable dose-dependent increase in acetylcholine receptors. This effect is probably dependent on ovotransferrin's ability to donate iron to the cells. In many respects ovotransferrin is similar to 'sciatin', a myotrophic protein isolated from chicken sciatic nerves.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003207:Conalbumin / Q000096:biosynthesis; D007700:Kinetics; D051379:Mice; D009132:Muscles / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D009474:Neurons / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism*; D014168:Transferrin / Q000096:biosynthesis*
D009419:Nerve Tissue Proteins; D014168:Transferrin; C026913:sciatin; D003207:Conalbumin