An assay for C1q biosynthesis in cultured human fibroblasts.
With a standard C1 haemolytic assay, cultured human fibroblasts were shown to synthesize and secrete haemolytically active C1 and C1q. An assay for detection and quantitation of intra-cellular biosynthesis and secretion of C1q was then developed, using Sepharose beads covalently coated with goat monospecific anti-C1q IgG. The molecular structure of fibroblast C1q was found to be unusual in 2 ways: firstly, it probably represented an enlarged pro-C1q, and secondly, the secreted molecule had a different structure from the cell associated molecule.
D001667:Binding, Competitive; D002478:Cells, Cultured; D003166:Complement Activating Enzymes / Q000096:biosynthesis*; D015922:Complement C1q; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000276:immunology; D006461:Hemolysis; D006801:Humans
D015922:Complement C1q; D003166:Complement Activating Enzymes