Urinary procoagulant behaves as tissue factor by promoting factor VIIa-catalyzed activation of factor X.
We purified the urinary procoagulant from frozen human urine by introducing phenyl-Sepharose hydrophobic chromatography. By this method, the apoprotein of the procoagulant and the lipid-like substance were separately recovered. Upon reassociation with the lipid-like substance or exogenous crude phospholipids, the apoprotein accelerated factor VIIa-catalyzed activation of factor X, probably by forming a stoichiometric complex with the catalytic enzyme. Thus the procoagulant was confirmed to be a tissue factor by its mode of participation in the blood coagulation mechanism.
D001059:Apoproteins / Q000652:urine; D001777:Blood Coagulation; D003208:Concanavalin A / Q000494:pharmacology; D005167:Factor VII / Q000494:pharmacology*; D015942:Factor VIIa; D005170:Factor X / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008757:Methylglucosides / Q000494:pharmacology; D010743:Phospholipids / Q000652:urine; D013925:Thromboplastin / Q000652:urine*
D001059:Apoproteins; D008757:Methylglucosides; D010743:Phospholipids; D003208:Concanavalin A; C027020:methylglucoside; D005167:Factor VII; D005170:Factor X; D013925:Thromboplastin; D015942:Factor VIIa