A simple procedure for freezing and storing lymphocyte panels in trays.
Lymphocytes from different donors may be frozen in Terasaki trays by a simple procedure. This method provides the tissue typing laboratory with a readily available panel that can be routinely used to test for lymphocytotoxic antibodies sera from patients waiting for kidney transplant, transplanted patients and polytransfused individuals. The advantage of this procedure is that expensive programmed freezing apparatus is not required.
D001793:Blood Preservation / Q000295:instrumentation / Q000379:methods*; D002469:Cell Separation; D002470:Cell Survival; D003601:Cytotoxicity Tests, Immunologic; D005615:Freezing; D006650:Histocompatibility Testing / Q000295:instrumentation*; D006801:Humans; D008214:Lymphocytes / Q000276:immunology* / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology