The occurrence of polysialogangliosides including ganglio-N-tetraose series in adult bovine nasal cartilage.
We extracted glycolipids from adult bovine nasal cartilage and purified some glycolipids by DEAE-Sephadex A-25 and Iatrobeads column chromatography. Cartilage contained 20 nmol of lipid bound sialic acid per gram wet tissue. The relative content of mono, di, tri, and tetrasialo gangliosides were 14%, 40%, 28% and 18%, respectively, as sialic acid content. We characterized some by examining carbohydrate composition, methylation analysis, sialidase treatment and mild acid hydrolysis. The ganglio-N-tetraose series, including GD1a, GD1b, GT1a, GT1b and GQ1b, was identified as one of the major ganglioside groups of this cartilage.
D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002356:Cartilage / Q000737:chemistry*; D002417:Cattle; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000302:isolation & purification*; D006017:Glycolipids / Q000302:isolation & purification; D009666:Nose; D013329:Structure-Activity Relationship
D002241:Carbohydrates; D005732:Gangliosides; D006017:Glycolipids