Antibody-dependent cell-mediated cytotoxicity against influenza virus-infected cells.
The three major immunocompetent cells in human peripheral blood (lymphocytes, neutrophils, and monocytes) were shown to be effector cells for antibody-dependent cell-mediated cytotoxicity (ADCC) against influenza virus-infected baby hamster kidney cells in vitro. Lymphocyte cytotoxicity was mediated by FcIgG receptor-bearing null cells and T gamma cells. These effector populations were best defined by HNK-1, a monoclonal antibody to human natural killer and ADCC-mediator cells. Antibody responsible for ADCC against influenza virus-infected cells was detectable in sera of young children after natural infection and after vaccination with inactivated and live attenuated viruses. ADCC antibody appeared before hemagglutination-inhibiting antibody and persisted for at least one year after vaccination with live attenuated vaccine. ADCC antibody was subtype-specific but quite broadly reactive within a subtype. Both hemagglutinin and neuraminidase were antigenic determinants for ADCC antibody. An anamnestic response to the original strain was observed after challenge with influenza virus of a heterologous subtype.
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000918:Antibody Specificity; D000920:Antibody-Dependent Cell Cytotoxicity; D002460:Cell Line; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D006224:Cricetinae; D006389:Hemagglutinins, Viral / Q000276:immunology; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D007223:Infant; D009980:Influenza A virus / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage; D007251:Influenza, Human / Q000276:immunology* / Q000517:prevention & control; D007694:Killer Cells, Natural / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D007141:Immunoglobulin Fc Fragments; D007252:Influenza Vaccines