title: Ca2+-free perfusion of isolated rat heart: early irreversible changes and discrepancy between functional impairments and release of cellular constituents.
abstract: The effects of repeated (10 X), brief Ca2+-free perfusions (20 to 120 s) on myocardial contractility, coronary flow and release of cellular constituents of isolated rat heart were investigated. Ten successive Ca2+-deprivations of 20 or 40 s had no irreversible influences on cardiac performance. Ca2+-deprivations, however, of 60 s or longer, resulted in a substantial depression of contractile activity during recovery, which effect was parallelled by the development of myocardial contracture. Both effects appeared to be additive in character (and thus irreversible) and were dependent upon the duration and number of Ca2+-deprivations. The effect on coronary flow was biphasic: after an initial, rapid increase coronary flow steadily declined and was almost normal at the end of the experiments. The release of cellular constituents into the coronary effluent only occurred during reperfusion with Ca2+ after Ca2+-free periods of 60 s or longer. This loss of cellular material from the heart was observed mainly after the first Ca2+-deprivation and was only small or even negligible during the subsequent Ca2+-deprivations. The overall release of cellular material was clearly dependent on the duration of the Ca2+-free period, but was badly related to the overall loss in contractile activity. It was concluded that a relatively brief Ca2+-free perfusion period may induce a small but irreversible damage to the heart. This damage becomes visible, and more deleterious, when the brief Ca2+-washout is repeated several times. In addition, it was concluded that Ca2+-free-induced functional impairments and the release of cellular constituents of the heart are badly correlated and may be caused by two separate, but probably interrelated, mechanisms.
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology*; D003326:Coronary Circulation; D006321:Heart / Q000502:physiology*; D006331:Heart Diseases / Q000209:etiology; D066298:In Vitro Techniques; D008297:Male; D009200:Myocardial Contraction; D009206:Myocardium / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D002118:Calcium