Residual minor histocompatibility genes contaminate the B10.AM congenic line: no evidence of C-region-controlled histoincompatibility.
After being primed in vivo and restimulated in vitro, cytolytic T lymphocytes (CTL) were produced in the strain combinations B10.AM anti-B10.A(1R) and B10.A(1R) anti-B10.AM. Although the two strains differ in the chromosomal interval between the E alpha and the D loci, the CTL are not directed against antigens controlled by loci in this interval. Instead, the CTL detect minor histocompatibility (H) antigens controlled by loci that are not linked to H-2. The recognition of the antigens detected by the B10.AM anti-B10.A(1R) CTL is restricted by the Kk and Db molecules, but the CTL also cross-react with the Dd molecule (or a molecule controlled by a locus closely linked to Dd). The recognition of the antigens detected by these two CTL behave as if controlled by alleles at the same minor H locus or loci. This locus is distinct from H-2, and the B10.AM congenic line apparently retained a C3H-derived allele at this locus.
D000483:Alleles; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains; D008912:Minor Histocompatibility Loci; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000911:Antibodies, Monoclonal