Removal of HLA-A,B antigens from platelets.
Recent evidence suggests that HLA-A,B antigens present on human platelets, are acquired from plasma. Using hypertonic acid chloroquine (200 mg/ml, pH 5.0, 1 hr, 4 degrees C), we demonstrate elution of most or all HLA-A2 and HLA-B7,40 immunoreactive material from platelets. Corresponding antigens on human lymphocytes were not affected by this treatment. These findings are consistent with the hypothesis that HLA-A,B antigens are adsorbed onto platelet surfaces.
D000911:Antibodies, Monoclonal / Q000276:immunology; D001792:Blood Platelets / Q000187:drug effects / Q000276:immunology*; D002738:Chloroquine / Q000494:pharmacology; D006680:HLA Antigens / Q000276:immunology*; D015789:HLA-A2 Antigen; D015235:HLA-B Antigens; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D015789:HLA-A2 Antigen; D015235:HLA-B Antigens; D002738:Chloroquine