title: Mg-2,4-divinyl pheoporphyrin a5: the product of a reaction catalyzed in vitro by developing chloroplasts.
abstract: The major product of an aerobic reaction mixture containing developing chloroplasts, Mg-protoporphyrin IX, S-adenosylmethionine, and other cofactors was isolated and purified. Structural studies using nuclear magnetic resonance confirmed earlier reports, based on fluorescence and absorption spectra, that this compound is Mg-2,4-divinyl pheoporphyrin a5. The molecular weight determined by secondary-ion mass spectroscopy further confirmed the assigned structure. Absorption and fluorescence spectra indicate that this compound is identical to that reported previously by various workers in less-purified biological extracts. The nuclear magnetic resonance spectrum of the Mg-free base also supports the assigned structure.
mesh_terms: D000332:Aerobiosis; D002734:Chlorophyll / Q000031:analogs & derivatives*; D002736:Chloroplasts / Q000378:metabolism*; D013058:Mass Spectrometry; D008848:Micromonosporaceae / Q000378:metabolism*; D010944:Plants / Q000378:metabolism; D011521:Protochlorophyllide / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D012242:Rhodobacter sphaeroides / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
chemical_list: D002734:Chlorophyll; C020301:2,4-divinylprotochlorophyllide; D011521:Protochlorophyllide