Cultured beating myocytes from neonatal rat heart as a model for the study of muscarinic cholinergic receptors.
Neonatal rat heart myocytes were isolated by digestion of the heart tissue with trypsin. After separation the cells were transferred to plastic cluster dishes where they formed a monolayer culture. After 72 hours in culture the myocytes showed a synchronous contraction and renovation of their membrane structures. Studies of muscarinic cholinergic receptors were carried out using (3H)-Quinuclidinyl benzilate directly on cells attached to the bottom of the dish. The presence of a specific muscarinic receptor on cultured beating myocytes was confirmed.
D000818:Animals; D002478:Cells, Cultured; D004837:Epinephrine / Q000494:pharmacology; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D011433:Propranolol / Q000494:pharmacology; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000378:metabolism*
D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate; D011433:Propranolol; D004837:Epinephrine