Preparation and examination of the oligosaccharides of serum alpha 1-antitrypsin PiZ.
Oligosaccharides were prepared from serum alpha 1-antitrypsin, PiM and PiZ, by hydrazinolysis and reacetylation with [3H]acetic anhydride. A comparison by gel permeation chromatography indicated that the two variants contain the same kinds of complex oligosaccharides. Specifically, serum PiZ does not contain mannose-rich oligosaccharides in spite of the presence of mannose-rich oligosaccharides in liver PiZ.
D000328:Adult; D002850:Chromatography, Gel; D006801:Humans; D008099:Liver / Q000378:metabolism; D008297:Male; D008358:Mannose / Q000378:metabolism; D009844:Oligosaccharides / Q000302:isolation & purification* / Q000378:metabolism; D000515:alpha 1-Antitrypsin / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics
D009844:Oligosaccharides; D000515:alpha 1-Antitrypsin; D008358:Mannose