title: Characterization of sea-urchin fibronectin.
abstract: Sea-urchin fibronectin from the ovary of the sea urchin Pseudocentrotus depressus bound to gelatin, fibrin and fibrinogen. After mild digestion of the protein labelled with 125I, a 195 000 Da domain was observed. Sea-urchin fibronectin was aggregated by spermine (1 mM) at neutral pH. When the concentration of spermine was decreased or increased, the aggregation was diminished. The addition of 1 M-NaCl or 4 M-urea inhibited the spermine-induced aggregation. Sea-urchin fibronectin mediated the spreading of baby-hamster kidney cells on the plastic surface.
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005337:Fibrin; D005340:Fibrinogen; D005353:Fibronectins / Q000494:pharmacology; D046911:Macromolecular Substances; D010053:Ovary / Q000032:analysis; D012617:Sea Urchins / Q000032:analysis*; D013096:Spermine; D014357:Trypsin
chemical_list: D005353:Fibronectins; D046911:Macromolecular Substances; D013096:Spermine; D005337:Fibrin; D005340:Fibrinogen; D014357:Trypsin