Analysis of doxylamine in plasma by high-performance liquid chromatography.
A rapid and sensitive high-performance liquid chromatographic (HPLC) assay for the quantitative determination of doxylamine in plasma is described. The drug levels of doxylamine in plasma were monitored after the oral administration of a single 25-mg tablet of doxylamine succinate to each of 20 male volunteers. The compound was extracted from the plasma samples, concentrated under a nitrogen stream, and analyzed by HPLC using normal-phase chromatography with detection at 254 nm. The detection limit is approximately 5 ng/ml.
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004319:Doxylamine / Q000097:blood*; D006801:Humans; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D011725:Pyridines / Q000097:blood*
D011725:Pyridines; D004319:Doxylamine