title: Unfolding pathway of myoglobin. Evidence for a multistate process.
abstract: The free energy of unfolding of horse myoglobin has been calculated from the denaturation pattern induced by guanidine hydrochloride as well as by acid. The delta GH2O, i.e., the value in the absence of denaturant obtained by using the two-state transition model, was found to be 25% lower than that determined from the acid denaturation pattern, i.e., 12.0 kcal/mol, although the extent of protein denaturation produced by acid was much lower. The amount of helical structure surviving the acid-induced conformational change was estimated to be 50% of that present in the native protein, and it could be destroyed only after exposure of myoglobin samples kept at pH 3.0 to concentrated guanidine. From the guanidine denaturation pattern at acidic pH, a further variation of free energy of unfolding of 5.5 kcal/mol could be calculated, thus indicating that the overall free energy of unfolding determined from the two consecutive processes corresponds to 17.5 kcal/mol. The discrepancy between the two sets of data, i.e., guanidine unfolding at neutral pH and acid unfolding followed by addition of denaturant, has been considered to depend on the general assumption that the guanidine unfolding of myoglobin is a two-state process in the transition region. According to the recent experimental evidence showing the occurrence of at least two molecular events during the guanidine unfolding of apomyoglobin [Colonna, G., Balestrieri, C., Bismuto, E., Servillo, L., & Irace, G. (1982) Biochemistry 21, 212-215], the guanidine denaturation pattern of myoglobin was analyzed in terms of two independent steps.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D019791:Guanidine; D006146:Guanidines; D006736:Horses; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008433:Mathematics; D009211:Myoglobin / Q000378:metabolism*; D011487:Protein Conformation; D013816:Thermodynamics
chemical_list: D006146:Guanidines; D009211:Myoglobin; D019791:Guanidine