[Development of preimplantation mouse embryos in diffusion chambers].
The diffusion chambers with the preimplantation CBA X C57BL mouse embryos (at the stages of blastomeres, morula, blastocyst) were placed in the abdominal cavity of males. Developmental peculiarities of the embryos, level of mitotic activity in their separate parts, as well as dynamics of the number of gigantic trophoblast cells and the volume of their nuclei have been studied. The development of the embryos in the diffusion chambers was shown to depend on the type of membranous filters: they developed from an earlier stage (stage of 4 blastomeres) on the nucleopore filters than on the millipore ones. The morphogenetic transformation proceeded in the preimplantation embryos at a slower rate resulting in the formation of two -and (in single cases) three-layered embryonic cylinder. Later the embryos were disorganized. The repeated passage of the disorganized embryo cells (six passages during the year) allowed to obtain structures similar with yolk sac carcinoma.
D000005:Abdomen; D000818:Animals; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000166:cytology / Q000502:physiology*; D047108:Embryonic Development; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008938:Mitosis; D011247:Pregnancy
Razvitie doimplantatsionnykh zarodysheĭ mysheĭ v diffuzionnykh kamerakh.