title: Development of intercellular junctions in the vestibular end-organ. A freeze-fracture study in the mouse.
abstract: The intercellular junctions and the tight junctions in particular are considered to be of great importance for the function of the inner ear. The two fluid compartments of the inner ear, the perilymphatic space and the endolymphatic space, need to be effectively separated from each other in order to maintain the ionic gradients between the two. The tight junctional structures have been described in mature animals of several species. In the present article the development and maturation of the intercellular junctions are described in the mouse embryo. Junctional elements are already present in the 12th gestational day otocyst. Over the next few days, the otocyst is differentiated into a cochlear portion and a vestibular portion. The tight junctions in the vestibular portion gradually attain their mature appearance. It seems as if the tight junctions of the supporting cells develop slightly faster than those of the hair cells. At the time of birth, all epithelial cells have obtained mature appearance. The tight junctions are fully developed on the supporting cells as well as the hair cells. Small gap junctions are present in the 14th gestational day specimens. Two days later the hair cells and the supporting cells are well differentiated; small to medium-sized gap junctions are present only on the supporting cells at this stage. At the time of birth larger gap junctional aggregates have developed on the supporting cells.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D005260:Female; D005614:Freeze Fracturing; D005865:Gestational Age; D007365:Intercellular Junctions / Q000502:physiology*; D051379:Mice; D008808:Mice, Inbred CBA; D009024:Morphogenesis; D011247:Pregnancy; D014722:Vestibule, Labyrinth / Q000196:embryology* / Q000648:ultrastructure