A quantitative method for determination of 5,6-dihydroxyindole-2-carboxylic acid using high-pressure liquid chromatography with fluorometric detection.
A method for the determination of the urinary melanocyte metabolite 5,6-dihydroxyindole-2-carboxylic acid is described. The catecholic indole is adsorbed onto alumina, chromatographed on a reverse-phase octadecyl silica column, and detected by fluorometry. The sensitivity of the method permits detection of 1 pmol injected.
D002851:Chromatography, High Pressure Liquid; D005470:Fluorometry; D006801:Humans; D007211:Indoles / Q000302:isolation & purification / Q000652:urine*; D008544:Melanocytes / Q000378:metabolism
D007211:Indoles; C030692:5,6-dihydroxy-2-indolylcarboxylic acid