T cell subsets in the tonsil in relation to age analyzed with monoclonal antibodies (anti-leu antibodies) and fluorescence activated cell sorter (FACS).
The ratio of the cells reacting to anti-leu 1, 3a and 4 antibodies is as low as that of T cells in the tonsil to those in the peripheral blood. The number of the tonsillar cells reacting to anti-leu 2a and 7 antibodies is still smaller. Fluorescence intensity of tonsillar lymphocytes reacting to leu (1, 2a, 3a, and 4) antibodies is lower than that of peripheral blood lymphocytes. Adult tonsillar lymphocytes have higher ratios of the cells reacting to anti-leu 1, 2a, 3a, 4 and 7 antibodies than child tonsillar lymphocytes and fluorescence intensity of the cells reacting to these anti-leu antibodies tended to be higher in adult tonsillar lymphocytes. These findings suggest less cytotoxic/suppressor T and NK/K function cells in the tonsil than in the peripheral blood. From these, it is conceivable that the tonsillar lymphocyte constitution becomes closer to that of peripheral blood as the child grows up. Further, immature T cells are present in the tonsil, though in a small number. This tendency is stronger in children than in adults.
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002469:Cell Separation; D002648:Child; D002675:Child, Preschool; D005434:Flow Cytometry; D006801:Humans; D014066:Palatine Tonsil / Q000276:immunology*; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D000911:Antibodies, Monoclonal