A general method for the transfer of cloned genes to plant cells.
This paper describes a method for the transfer to plant cells of any cloned gene, regardless of its termini or internal restriction enzyme cleavage sites. A broad host-range intermediate vector, pGV1117, was constructed containing HindIII-23, a right-end T-region fragment of the nopaline plasmid pTiC58. Using in vivo protection by EcoRI methylase and EcoRI linker ligation, a fragment of rabbit chromosomal DNA, carrying the beta-globin gene, was inserted into plasmid pGV1117. Following transmission to Agrobacterium tumefaciens, insertion of the gene into the T-region of pTiC58 occurred via in vivo recombination. Infection of axenic tobacco seedlings resulted in the transfer to the plant genome of an intact beta-globin gene, as part of the T-DNA. Although the gene was stably maintained during tissue culture, beta-globin-specific transcripts were not detected in the transformed plant cells.
D003001:Cloning, Molecular / Q000379:methods*; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics; D010941:Plant Tumors / Q000235:genetics; D010944:Plants / Q000235:genetics*; D010957:Plasmids; D011995:Recombination, Genetic; D012231:Rhizobium / Q000235:genetics*; D014158:Transcription, Genetic
D005914:Globins