Fluorometric determination of carbodiimides with trans-aconitic acid.
A new fluorometric method for the determination of carbodiimides with trans-aconitic acid as a reagent has been developed. With the present method, as little as 50 pmol of carbodiimides can be determined. The relative standard deviations obtained with 25 nmol of dicyclohexylcarbodiimide (DCC), diisopropylcarbodiimide (DIC), and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide HCl (EDC) were 1.35, 1.41, and 2.72% (n = 10), respectively.
D000085:Acetates; D019342:Acetic Acid; D000156:Aconitic Acid; D002234:Carbodiimides / Q000032:analysis*; D011725:Pyridines; D013050:Spectrometry, Fluorescence; D014233:Tricarboxylic Acids
D000085:Acetates; D002234:Carbodiimides; D011725:Pyridines; D014233:Tricarboxylic Acids; D000156:Aconitic Acid; C023666:pyridine; D019342:Acetic Acid