Identification of some OH radical-induced products of lysozyme.
OH radical reactions with lysozyme in gamma-irradiated N2O saturated aqueous solutions caused formation of allo-threonine, alpha-amino-n-butyric acid, o- and m- tyrosines, and 2- and 3-hydroxytyrosines. These identified radiolytic products were characterized by capillary gas chromatography-mass spectrometry as their trimethylsilyl derivatives after HCl-hydrolysis of irradiated lysozyme. Their initial G-values were also determined using gas chromatography. The possible use of these radiolytic products as monitors of radiation-induced damage to proteins and the sites of attack are also discussed.
D000596:Amino Acids / Q000032:analysis; D004307:Dose-Response Relationship, Radiation; D005410:Flame Ionization; D005519:Food Preservation / Q000379:methods; D005609:Free Radicals; D005720:Gamma Rays; D008401:Gas Chromatography-Mass Spectrometry; D009113:Muramidase / Q000528:radiation effects*
D000596:Amino Acids; D005609:Free Radicals; D009113:Muramidase