Mitogenic effect of anti-Friend leukemia virus antiserum on T cells.
It has been previously reported that goat- and rat antisera directed against Friend leukemia virus (aFLV) are mitogenic for some B cells, but not for T cells. Here we report that activation of T cells by concanavalin A (Con A) rendered T cells responsive to the mitogenic activity of aFLV. This activity was contained in the immunoglobulin fraction and could be absorbed by purified FLV preparations. Optimal conditions for measuring the mitogenc activity of aFLV include 48 h preincubation of thymocytes with 3 micrograms/ml Con A followed by reculturing the activated thymocytes for 28 h with aFLV. The acquisition of an aFLV-responsive state was dependent on early protein synthesis during the Con A-induced activation period. aFLV did not substitute for interleukin 2 (IL2) in a costimulator assay. Evidence is presented that aFLV acted in a cocultivation assay via growth factor(s). In contrast to control cultures, aFLV-treated lymphoblasts contained, in their supernatants, IL2 activity as demonstrated by their effect on an IL2-dependent T cell line. The data suggest that aFLV acted upon activated T cells by enhancing the endogenous production and/or release of IL2.
D000818:Animals; D000914:Antibodies, Viral / Q000502:physiology*; D005622:Friend murine leukemia virus / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007376:Interleukin-2 / Q000502:physiology; D007700:Kinetics; D007942:Leukemia, Experimental / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D011691:Puromycin / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*
D000914:Antibodies, Viral; D007140:Immunoglobulin Fab Fragments; D007376:Interleukin-2; D011691:Puromycin