title: Comparison of the effect of temperature on kidney cortex mitochondria from rabbit, dog, pig, and human: Arrhenius plots of ADP-stimulated respiration.
abstract: The effect of temperature on the rate of ADP-stimulated respiration of mitochondria from dog, rabbit, pig, and human kidney cortex mitochondria was plotted according to the Arrhenius relationship. The temperature at which the plot demonstrated a break was at 15 degrees C for mitochondria from dog, pig, and human kidneys. The discontinuity occurred at 10 degrees C or less for mitochondria from rabbit kidneys. This difference suggests that mitochondria from rabbit kidneys undergo a lipid-phase transition at lower temperatures than for other species commonly used in experimental renal preservation. The implications of this difference suggest caution in using results obtained with rabbit kidneys for comparison to results obtained from hypothermic renal preservation of other species kidneys. Apparent fluidization of dog kidney mitochondrial membranes with adamantine abolished the discontinuity in the Arrhenius plot.
mesh_terms: D000218:Adamantane / Q000494:pharmacology; D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000818:Animals; D004285:Dogs; D006801:Humans; D007672:Kidney Cortex / Q000378:metabolism*; D008560:Membrane Fluidity / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism*; D009926:Organ Preservation; D010101:Oxygen Consumption / Q000187:drug effects*; D011817:Rabbits; D013045:Species Specificity; D013268:Stimulation, Chemical; D013552:Swine; D013696:Temperature
chemical_list: D000244:Adenosine Diphosphate; D000218:Adamantane