[Purification of commercial preparations of NADP from AMP].
A method in described for elimination of AMP from commercially available preparations of NADP. The method enables to obtain desalted NADP preparations of 98-99.5% purity with a yield of 70-80% using only one chromatographic step. The procedure included anion exchange chromatography on Dowex 1, HCOO- -form. This chromatographic step completely separated AMP from NADP in a gradient of HCOOH concentrations from 0 to 2 N, and NADP was eluted by 1.5 N HCOOH containing 0.15 N HCOOK; during subsequent precipitation and washing of NADP by means of acetone HCOOK remained in a solution. Addition of HCOOK was required for a decrease in NADP elution volume which was important for the subsequent precipitation with acetone.
D000249:Adenosine Monophosphate / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange / Q000379:methods; D007202:Indicators and Reagents; D009249:NADP / Q000302:isolation & purification* / Q000592:standards
D007202:Indicators and Reagents; D000249:Adenosine Monophosphate; D009249:NADP
Ochistka kommercheskikh preparatov NADF ot AMF.