Malathion and phenthoate carboxylesterase activities in pulmonary alveolar macrophages as indicators of lung injury.
Malathion and phenthoate carboxylesterase activities were investigated in pulmonary alveolar macrophages (PAM) in Sprague-Dawley rats. PAM was found to be capable of hydrolyzing phenthoate at a faster rate than malathion. Oral administration to rats with O,O,S-trimethyl phosphorothioate (OOS-Me), a pneumotoxic impurity present in technical grades of malathion and phenthoate, increased the activities of these esterases in PAM without affecting an activity in lung microsomal carboxylesterase. The time course study indicated that this increase was maximal on Day 1 following treatment with OOS-Me at 20 and 40 mg/kg of doses. To assess the usefulness of measuring these esterases in PAM as an indicator of lung damage, paraquat and bromobenzene were administered to rats with treatment regimens which have been shown previously to result in histopathologically demonstrable pneumotoxicity. Malathion and phenthoate carboxylesterase activities in PAM were increased by two- to threefold following treatment with paraquat or bromobenzene. These treatments also increased lung microsomal malathion carboxylesterase activity by threefold. Furthermore, infection of rats with Pseudomonas aeruginosa by intratracheal inoculation increased malathion and phenthoate carboxylesterase activities in PAM by two- to threefold without increasing these activities in lung microsomes. These results indicate that PAM may play a significant role in detoxifying airborne malathion and phenthoate when inhaled. Furthermore, the activities of malathion and phenthoate carboxylesterases may be useful for detecting lung injury produced by pneumotoxic chemicals as well as bacterial infection.
D000818:Animals; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D007306:Insecticides / Q000378:metabolism*; D008168:Lung / Q000187:drug effects*; D008264:Macrophages / Q000201:enzymology*; D008297:Male; D008861:Microsomes / Q000201:enzymology; D063086:Organothiophosphates / Q000633:toxicity; D009946:Organothiophosphorus Compounds / Q000378:metabolism*; D010269:Paraquat / Q000633:toxicity; D011650:Pulmonary Alveoli / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
D007306:Insecticides; D063086:Organothiophosphates; D009946:Organothiophosphorus Compounds; C023890:O,O,O-trimethyl phosphorothioate; D002265:Carboxylic Ester Hydrolases; C021528:malathion esterases; D043182:Carboxylesterase; C008838:phenthoate; D010269:Paraquat