[The primary structure of hemoglobins of the rock hyrax (Procavia habessinica, Hyracoidea): insertion of glutamine in the alpha chains].
The chromatography of the hemoglobin of the rock hyrax (Procavia habessinica) gives two components (73% HbI and 27% HbII). The amino-acid analysis and the sequences of the globin chains elucidated with the phenylthiohydantoin method, did not show any differences between the alpha I and alpha II or beta I and beta II chains, respectively. The different chromatographical behaviour cannot be explained. After chain separation by chromatography on CM-52 cellulose, all four primary structures were elucidated automatically in a sequenator on the chains and the tryptic peptides. In 20% of the beta I chains the N-terminal valine was blocked by acetyl. The alignment was performed by homology with the chains of human adult hemoglobin. The alpha chain of the rock hyrax has 142 amino-acid residues, i.e. one residue more than normal mammalian alpha chains, caused by an insertion of glutamine in the GH region supposed between positions 115 and 116. A comparison of human and hyrax hemoglobins shows an exchange of 21 amino-acid residues in the alpha chains and of 24 in the beta chains. Some substitutions in alpha 1 beta 1 contacts and in the surrounding of the heme are not supposed to effect the function of the hemoglobin. The phylogenetic relationship between the rock hyrax and the Indian elephant (Elephas maximus) on the one hand and with some Perissodactyla on the other, is discussed. Up to now the exchanges of alpha 110(G17)Ala leads to Ser and beta 56(D7)Gly leads to His have only been found in hyrax and elephant. This indicates a certain relationship between Hyracoidea and Proboscidea.
D000595:Amino Acid Sequence; D000818:Animals; D004606:Elephants / Q000097:blood; D005973:Glutamine; D006441:Hemoglobin A; D006454:Hemoglobins / Q000302:isolation & purification; D006801:Humans; D046911:Macromolecular Substances; D008322:Mammals / Q000097:blood*; D010527:Perissodactyla / Q000097:blood; D010802:Phylogeny; D013045:Species Specificity
D006454:Hemoglobins; D046911:Macromolecular Substances; D005973:Glutamine; D006441:Hemoglobin A
Die Primärstruktur des Hämoglobins vom Abessinischen Klippschliefer (Procavia habessinica, Hyracoidea): insertion von Glutamin in den alpha-Ketten.