A direct solubilization procedure for the determination of DNA and protein in cultured fibroblast monolayers.
A procedure for the direct solubilization of fibroblast monolayers allowing precise determination of DNA and protein content has been developed. This simple procedure is applicable to the analysis of these macromolecules in fibroblast cultures containing small numbers of cells (less than 1 X 10(5].
D002021:Buffers; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis*; D005347:Fibroblasts / Q000032:analysis*; D006801:Humans; D007211:Indoles; D011092:Polyethylene Glycols; D011506:Proteins / Q000032:analysis*; D012972:Sodium Hydroxide; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
D002021:Buffers; D007211:Indoles; D011506:Proteins; D011092:Polyethylene Glycols; C007293:DAPI; D012972:Sodium Hydroxide; D004247:DNA