The use of protein beads as immunoadsorbent for the column fractionation of lymphocytes.
Beads of calf serum proteins (CSB) with antigen-antibody complexes or antigen by activation with glutaraldehyde were used as immunoadsorbents for the fractionation of mouse lymphocytes. T-lymphocytes could be separated by this method with high purity. The precursors of antibody-forming cells were completely eliminated from spleen and bone marrow cell suspensions. The unspecific binding of lymphocytes to CBS or Sepharose was similar and not selective for T or B lymphocytes.
D000818:Animals; D000921:Antibody-Producing Cells; D000936:Antigen-Antibody Complex; D001798:Blood Proteins; D002469:Cell Separation / Q000379:methods*; D006412:Hematopoietic Stem Cells; D007164:Immunosorbents; D008214:Lymphocytes; D051379:Mice; D013601:T-Lymphocytes
D000936:Antigen-Antibody Complex; D001798:Blood Proteins; D007164:Immunosorbents