Immunoregulatory factors from a human macrophage-like cell line. II. A human T-cell lymphokine-induced suppressor factor for lymphocyte proliferation.
It has been demonstrated that the human histiocytic lymphoma-derived cell line U937, which has monocytoid characteristics, responds to a concanavalin A-induced T-cell-derived suppressor supernatant (T-SFS) with the release of a factor markedly suppressing mitogen-stimulated proliferation of normal peripheral blood lymphocytes. The suppressor material is not dialyzable, appears within 2 hr of exposure of U937 cells to the T-SFS, persists for at least 24 hr, and has a Mr of approximately 40,000 by gel chromatography. The suppressor factor does not affect the proliferation of continuous T- and B-lymphoid cell lines, distinguishing it from the inhibitor of DNA synthesis also released by U937, but appears to be specific for a stage of activation of normal lymphocytes that is independent of (a) utilization of interleukin-2 and (b) inhibition of production of interleukin-2.
D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000494:pharmacology*; D008223:Lymphoma; D008264:Macrophages / Q000276:immunology*; D008970:Molecular Weight; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013491:Suppressor Factors, Immunologic; D013601:T-Lymphocytes / Q000276:immunology
D007376:Interleukin-2; D008222:Lymphokines; D011043:Pokeweed Mitogens; D013491:Suppressor Factors, Immunologic; D003208:Concanavalin A