title: Immunoelectrophoretic study of the effect of radiation on protein mixtures.
abstract: This paper demonstrates the suitability of immunoelectrophoretic methods in studies of radiation effects on protein mixtures. Single serum proteins (IgG, albumin, transferrin), albumin-transferrin mixtures and human serum were irradiated with doses of 3-52 kGy. The irradiated proteins were analysed using rocket-, crossed-, crossed-with-intermediate-gel, and fused-rocket immunoelectrophoretic methods. Using crossed immunoelectrophoresis with intermediate gel, complex formation between albumin and transferrin was observed. Gel filtration monitored by fused-rocket immunoelectrophoresis was demonstrated to be a most promising method for studying protein degradation and hybrid formation.
mesh_terms: D001798:Blood Proteins / Q000528:radiation effects; D003037:Cobalt Radioisotopes; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D007074:Immunoglobulin G / Q000528:radiation effects; D010315:Particle Accelerators; D055503:Protein Multimerization / Q000528:radiation effects; D011506:Proteins / Q000528:radiation effects*; D012709:Serum Albumin / Q000528:radiation effects; D014168:Transferrin / Q000528:radiation effects
chemical_list: D001798:Blood Proteins; D003037:Cobalt Radioisotopes; D007074:Immunoglobulin G; D011506:Proteins; D012709:Serum Albumin; D014168:Transferrin