Intact rat liver nuclei catalyze adriamycin irreversible interactions with DNA and nuclear proteins.
Male rat liver intact nuclear preparations are able to metabolize adriamycin to reactive species that irreversibly interact with nuclear DNA and proteins in the presence of reduced NADPH. This process was not inhibited by 1 mM SKF-525A, suggesting that a nuclear monooxygenase enzymatic system was not involved.
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004317:Doxorubicin / Q000378:metabolism / Q000633:toxicity*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D009249:NADP / Q000378:metabolism; D009698:Nucleoproteins / Q000378:metabolism*; D011335:Proadifen / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D009698:Nucleoproteins; D009249:NADP; D004317:Doxorubicin; D004247:DNA; D011335:Proadifen