Preparation of F(ab')2 fragments of immunoglobulin G.
We describe a simple protocol for the preparation of F(ab')2 fragments of immunoglobulin G, based upon the known Fc- binding properties of protein A-Sepharose. The fragment preparations of xenogeneic and allogeneic anti-IgG were noncytotoxic to intact target cells, and were able to block the cytotoxicity of intact antibody. This method should therefore be useful for functional studies not requiring biochemical homogeneity.
D000818:Animals; D000915:Antibody Affinity; D003602:Cytotoxicity, Immunologic; D007140:Immunoglobulin Fab Fragments / Q000302:isolation & purification*; D007128:Immunoglobulin Fragments / Q000302:isolation & purification*; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D013154:Spleen / Q000276:immunology
D007140:Immunoglobulin Fab Fragments; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G