title: The presence of phospholipase A and lysophospholipase activities in culture supernatant fluid from Alteromonas espejiana.
abstract: Culture supernatants from late Log phase cultures of Alteromonas espejiana hydrolyzed phosphatidylinositol to glycerylphosphorylinositol and free fatty acid. No lysophosphatidylimositol was detected. The phospholipase activity degraded up to 50% of the substrate and displayed a broad pH optimum from 6.5-8.5. The activity was slightly stimulated in the presence of either Mg2+ or Ca2+, but was not inhibited by EDTA. The apparent Km for phosphatidylinositol was 1.5 mM. Culture supernatants also contained deacylating activity which released fatty acid from phosphatidylcholine and erythrocyte ghosts.
mesh_terms: D001419:Bacteria / Q000201:enzymology*; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008245:Lysophospholipase / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*
chemical_list: D003470:Culture Media; D010716:Phosphatidylinositols; D010740:Phospholipases; D010741:Phospholipases A; D008245:Lysophospholipase