Cardiac inhibitory action of constituents of the marine green alga Ulva pertusa.
As part of a search for substances from marine organisms, which exhibit inotropic effects, the chemical constituents of the marine green alga, Ulva pertusa were investigated. The fractionated extract was tested for inotropic effects on the isolated guinea pig atria. The aqueous layer obtained from the acetone extract of fresh algae was concentrated, and the residue was extracted with methanol. The methanolic extract was fractionated by chromatography using mixtures of aqueous methanol. Elution with 50% aqueous methanol afforded material that had a significant negative inotropic effect. Further purification of this material by high-performance liquid chromatography (HPLC) using methanol-water (2:5) afforded crystalline adenosine, which was shown to be the active substance of U. pertusa, causing a negative inotropic action.
D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D000460:Chlorophyta / Q000032:analysis*; D003864:Depression, Chemical; D006168:Guinea Pigs; D066298:In Vitro Techniques; D009200:Myocardial Contraction / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*
D010936:Plant Extracts; D000241:Adenosine