title: Dependence on cations for the binding activity of lectins as determined by affinity electrophoresis.
abstract: The metal ion content of eighteen different lectins was determined. The lectins were demetallized and the binding activity of native and demetallized forms were investigated using non-denaturing polyacrylamide affinity gel electrophoresis. The binding activities of all lectins were dependent on their metal ion content; when the cations were removed the lectins lost their carbohydrate binding activity. There was a marked difference in the strength with which lectins bind divalent cations.
mesh_terms: D002412:Cations; D004589:Electrophoresis, Disc; D037102:Lectins; D008670:Metals / Q000032:analysis*; D011485:Protein Binding; D013045:Species Specificity; D013054:Spectrophotometry, Atomic; D013329:Structure-Activity Relationship
chemical_list: D002412:Cations; D037102:Lectins; D008670:Metals