Cosynthesis and protoplast fusion by mutants of bialaphos (AMPBA) producing Streptomyces hygroscopicus.
Cosynthesis in mixed culture and protoplast fusion of non-producing mutants of Streptomyces hygroscopicus which may produce biosynthetic intermediates of bialaphos (AMPBA) were studied. Non-producing mutants were obtained by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) treatment, and six stable non-producing mutants were used for cosynthesis and protoplast fusion studies. The biosynthetic block sites of the non-producers were consistent with studies of the biosynthetic pathway of AMPBA.
D009154:Mutation; D009943:Organophosphorus Compounds / Q000096:biosynthesis*; D011523:Protoplasts / Q000502:physiology*; D011995:Recombination, Genetic; D013045:Species Specificity; D013302:Streptomyces / Q000235:genetics* / Q000254:growth & development
D009943:Organophosphorus Compounds; C003122:bialaphos