Anti-oxazolone hybridomas and strain distribution of the oxazolone idiotype.
Antibodies produced in the primary response to the hapten 2-phenyloxazolone (OX) express a cross-reactive idiotype in BALB/c and DBA/2 mice. We studied the response in hyperimmunized mice, using ascites produced after multiple immunizations with an OX conjugate and by generating monoclonal antibodies. A competitive radioimmunoassay was developed using a rabbit anti-idiotype antiserum raised against purified hyperimmune anti-OX antibodies. Mice from strains including CBA, C3H/He and B10.BR expressed all the determinants found in BALB/c serum, although at a lower titre. C57BL/6 mice, however, only expressed some of the BALB/c determinants. We isolated a monoclonal antibody, 1F9, which expressed some of the determinants found in BALB/c serum. All the cross-reactive idiotopes expressed on 1F9 were also expressed in the above strains including C57BL/6. Almost every BALB/c anti-OX antibody also expressed the 1F9 determinants. There are thus determinants of the BALB/c OX idiotype which are expressed in a number of different strains previously thought to be negative for the idiotype.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000918:Antibody Specificity; D001202:Ascitic Fluid / Q000276:immunology; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D003429:Cross Reactions; D006825:Hybridomas / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010080:Oxazoles / Q000276:immunology*; D010081:Oxazolone / Q000031:analogs & derivatives / Q000276:immunology*; D013045:Species Specificity
D000888:Antibodies, Anti-Idiotypic; D007106:Immune Sera; D007130:Immunoglobulin Idiotypes; D010080:Oxazoles; C018361:2-phenyloxazolone; D010081:Oxazolone