title: Steroid metabolism in fish. II. Testosterone metabolites in the bile of the marine winter flounder Pseudopleuronectes americanus and the freshwater Atlantic Salmon Salmo salar.
abstract: Intraarterial injection of [3H]testosterone in the flounder Pseudopleuronectes americanus and the landlocked salmon Salmo salar resulted in the concentration of radioactive catabolites in the gall bladder bile. The catabolites were quantitatively conjugated with glucuronic acid. In flounder bile, three steroids, testosterone, 5 beta-dihydrotestosterone and 5 beta-androstane-3 alpha,17 beta-diol accounted for most of the radioactivity. Quantitatively the concentration of 5 beta-dihydrotestosterone usually exceeded that of the other two compounds. In salmon bile, 5 alpha-dihydrotestosterone was also present as a minor catabolite and the dihydrotestosterones accounted for approximately one-half the total radioactivity. In both species there was evidence for trace amounts of 5 beta-androstane-3 beta,17 beta-diol, 5 alpha-androstane-3 alpha,17 beta-diol, and 5 alpha-androstane-3 beta,17 beta-diol but no evidence for 5 beta- or 5 alpha-androstanolones, 4-androstene-3 alpha,17 beta-diol, or its 3 beta-epimer. There were no obvious sex-related differences in the composition of the biliary testosterone metabolites.
mesh_terms: D015113:Androstane-3,17-diol / Q000378:metabolism; D000818:Animals; D001646:Bile / Q000378:metabolism*; D013196:Dihydrotestosterone / Q000378:metabolism; D005260:Female; D005399:Fishes / Q000378:metabolism*; D008297:Male; D012474:Salmon / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism*
chemical_list: D013196:Dihydrotestosterone; D015113:Androstane-3,17-diol; D013739:Testosterone