Effect of cobalt chloride and 3-amino-1,2,4-triazole on the induction of cytochrome P-450 synthesis by phenobarbitone in rat liver.
Administration of cobalt chloride and 3-amino-1,2,4-triazole leads to a suppression of phenobarbitone-mediated increase in total cytochrome P-450 as well as cytochrome P-450b contents of the liver. This suppression is due to a decrease in the content of the protein species which is the result of a decrease in its rate of synthesis as measured in vivo and in vitro. Cobalt chloride as well as 3-amino-1,2,4-triazole treatments lead to a decrease in the translatability of cytochrome P-450b RNA without affecting total protein synthesis. It is a possibility that a small pool of heme regulates the RNA levels for the cytochrome P-450 species.
D000640:Amitrole / Q000494:pharmacology*; D000818:Animals; D003035:Cobalt / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000302:isolation & purification; D004790:Enzyme Induction / Q000187:drug effects; D006418:Heme / Q000096:biosynthesis / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D010634:Phenobarbital / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects; D051381:Rats; D014230:Triazoles / Q000494:pharmacology*
D014230:Triazoles; D003035:Cobalt; D006418:Heme; D003577:Cytochrome P-450 Enzyme System; C018021:cobaltous chloride; D010634:Phenobarbital; D000640:Amitrole