Induction of fibroblast proliferation by human mononuclear leukocyte-derived proteins.
Human peripheral blood mononuclear leukocytes stimulated by T cell antigen or mitogen release soluble factors that induce mitogenesis of dermal fibroblasts in vitro. Fractionation of supernatants from antigen-stimulated mononuclear leukocyte cultures by Sephadex G-100 yielded 2 major peaks of fibroblast proliferation activity (approximately 60,000 and approximately 16,000 MW). Both proliferation factors are heat stable (56 degrees C for 45 minutes) proteins. These data show that mononuclear leukocytes, under appropriate conditions, can release soluble mediators that can stimulate fibroblast proliferation; such leukocytes may also play a role in expanding fibroblast populations adjacent to certain cell-mediated immune reactions in vivo.
D001798:Blood Proteins / Q000032:analysis / Q000494:pharmacology*; D002455:Cell Division; D002478:Cells, Cultured; D002498:Centrifugation; D002850:Chromatography, Gel; D005347:Fibroblasts / Q000166:cytology*; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D008934:Mitogens / Q000494:pharmacology; D009000:Monocytes / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology; D014357:Trypsin / Q000494:pharmacology
D001798:Blood Proteins; D008934:Mitogens; D014357:Trypsin