A monoclonal antibody (100C5) to the Lyt-2-T cell population expanding in MRL/Mp-lpr/lpr mice detects a surface antigen normally expressed on Lyt-2+ cells and B cells.
MRL/Mp-lpr/lpr (MRL-lpr) mice develop a generalized lymphadenopathy reflecting the expansion of a Lyt-2- T cell population. The present report describes the pattern of reactivity of a xenogeneic monoclonal antibody (mAb 100C5) directed against this T cell population. By single- and two-color flow cytofluorometry analysis this mAb was found to stain brightly 80-90% of T cells in enlarged MRL-lpr lymph nodes. In contrast lymph node T cells from congenic MRL-MP-+/+ (MRL-+) mice were either unstained (70%) or weakly stained (30%), these latter cells being mostly Lyt-2+. Unexpectedly, all lymph node B cells from MRL-+ or MRL-lpr mice were as strongly 100C5+ as MRL-lpr T cells. Similar observations were made in C57BL/6-lpr/lpr and C57BL/6-+/+ mice. Molecular weight determination suggested that the 100C5 mAb binds to the same molecule (Mr = 220 000) on MRL-lpr T cells and normal B cells.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology; D002454:Cell Differentiation; D007518:Isoantibodies / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008817:Mice, Mutant Strains / Q000276:immunology*; D008970:Molecular Weight; D010641:Phenotype; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007518:Isoantibodies; C029805:Lyt antibodies