title: An enzymatic rationale for the randomization of the positional distribution of fatty acids in phospholipids of ascites hepatoma AH 130.
abstract: Fatty acids present in glycerophospholipids isolated from Yoshida ascites hepatoma AH 130 are more randomly distributed among the 1- and 2-positions than are fatty acids of normal liver phospholipids. The relative abundance of unsaturated fatty acids at the 1-position was ascribed to the lower palmitate-specific glycerophosphate acyltransferase activity in mitochondria of the hepatoma cells, an observation supporting the conclusion put forward for the similar randomization observed in Ehrlich ascites cells (Haldar, D., Tso, W.-W. and Pullman, M.E. (1979) J. Biol. Chem. 254, 4502-4509). The relative abundance of saturated fatty acids at the 2-position could be ascribed to the relatively lower acyl-CoA:1-acyl-glycerophosphocholine acyltransferase activity and to the change in the selectivity of the hepatoma acyl-CoA:1-acyl-glycerophosphate acyltransferase system into the lung type. The relatively lower selectivity for arachidonoyl-CoA as compared with oleoyl-CoA of the 1-acyl-glycerophosphocholine acyltransferase system is consistent with the decrease in polyenoic fatty acid content at the 2-position of the hepatoma phospholipids.
mesh_terms: D008243:1-Acylglycerophosphocholine O-Acyltransferase; D000217:Acyltransferases / Q000494:pharmacology; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000201:enzymology; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats
chemical_list: D005227:Fatty Acids; D010743:Phospholipids; D000217:Acyltransferases; D008243:1-Acylglycerophosphocholine O-Acyltransferase