Influence of phospholipids on the activity of phosphate-dependent glutaminase in extracts of rat liver mitochondria.
Liver glutaminase can be solubilized from frozen-and-thawed mitochondria by treatment with phospholipase A2. Solubilization by this technique markedly changes the kinetic properties of the enzyme. The properties of the membrane-bound form of the enzyme are partially restored by adding phosphatidylcholine or phosphatidylethanolamine to the phospholipase extract. It is concluded that the kinetic properties of liver glutaminase are a function of the interaction of this enzyme with membrane phospholipids.
D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D005972:Glutaminase / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008563:Membrane Lipids / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology*; D010710:Phosphates / Q000494:pharmacology; D010741:Phospholipases A / Q000494:pharmacology; D054467:Phospholipases A2; D010743:Phospholipids / Q000494:pharmacology*; D051381:Rats
D008563:Membrane Lipids; D010710:Phosphates; D010743:Phospholipids; D010741:Phospholipases A; D054467:Phospholipases A2; D005972:Glutaminase