Rapid diagnosis of congenital adrenal hyperplasia by high performance liquid chromatography.
This manuscript describes methods for the quantitation of serum concentrations of 17-hydroxyprogesterone and 11-deoxycortisol (Compound S) employing reverse-phase high-performance liquid chromatography (HPLC) and ultraviolet (UV) absorbance which are applicable to the diagnosis of congenital adrenal hyperplasia due to deficiencies of 21- and 11-hydroxylase activities, respectively. These methods are simple, specific, precise and rapid. Data obtained by the HPLC-UV methods are highly correlated (p less than 0.001) with radioimmunoassay measurements.
D015065:17-Hydroxycorticosteroids / Q000097:blood*; D019326:17-alpha-Hydroxyprogesterone; D000293:Adolescent; D000312:Adrenal Hyperplasia, Congenital / Q000097:blood / Q000175:diagnosis*; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D003350:Cortodoxone / Q000097:blood*; D006801:Humans; D006908:Hydroxyprogesterones / Q000097:blood*; D007223:Infant; D007231:Infant, Newborn; D011863:Radioimmunoassay
D015065:17-Hydroxycorticosteroids; D006908:Hydroxyprogesterones; D019326:17-alpha-Hydroxyprogesterone; D003350:Cortodoxone