A radioisotopic method for the determination of S-adenosyl-L-homocysteine in tissues in the 10(-7) M range.
S-Adenosyl-L-homocysteine is able to bind to brain membranes. We used this characteristic to measure the level of S-adenosyl-L-homocysteine in rat brain tissue. The method is rapid, at the same time very sensitive (down to 10(-7) M) and specific.
D000818:Animals; D001923:Brain Chemistry; D006710:Homocysteine / Q000031:analogs & derivatives*; D008433:Mathematics; D008832:Microchemistry / Q000379:methods; D051381:Rats; D012435:S-Adenosylhomocysteine / Q000032:analysis*
D006710:Homocysteine; D012435:S-Adenosylhomocysteine