Antithrombin III and plasminogen: a comparison of two synthetic substrate-based methods.
We have evaluated and compared the DuPont aca and the KABI/CentrifiChem synthetic substrate-based methods for the quantitation of plasma plasminogen (PLG) and antithrombin III (AT III). Method precision was evaluated using normal human pooled plasma (NHP) as sample. Within-day and day-to-day PLG assays generated a CV of 2.0% and 2.9%, respectively, for the aca method. For the KABI/CentrifiChem PLG method, within-run (within-rotor) analysis produced a CV of 1.7%, and day-to-day analysis produced a CV of 5.3%. The relationship between the aca (y axis) and the KABI/CentrifiChem (x axis) methods for PLG was found to be y = 0.99X - 1.4%, r = 0.979. Within-day precision for AT III by the aca method generated a CV of 4.6%, and day-to-day analysis produced a CV of 7.0%. The KABI/CentrifiChem AT III method generated a CV of 4.2% and 4.4% for within-run and day-to-day assays, respectively. The AT III correlation demonstrated a relationship of y = 0.84X + 15.2%, r = 0.952. The AT III and PLG reference ranges as established in our laboratory for healthy adults, pregnant women, and for cord blood specimens also are presented.
D000328:Adult; D000990:Antithrombin III / Q000032:analysis*; D001778:Blood Coagulation Disorders / Q000097:blood / Q000175:diagnosis*; D001780:Blood Coagulation Tests / Q000379:methods* / Q000592:standards; D002863:Chromogenic Compounds; D005260:Female; D006487:Hemostasis; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D010958:Plasminogen / Q000032:analysis*; D011247:Pregnancy; D012016:Reference Values
D002863:Chromogenic Compounds; D000990:Antithrombin III; D010958:Plasminogen