Glucocorticoid receptor in chick erythrocytes.
Glucocorticoid receptor in chick erythrocytes has been characterized. The Scatchard plot analysis of (3H)-dexamethasone binding to red cells showed a single class of binding sites. The apparent Kd of (3H)-dexamethasone binding by whole cell assay was 0.33 nM and the maximum binding capacity was 5.1 fmole/10(7) cells. The apparent Kd of (3H)-dexamethasone binding to cytosol receptor was 0.48 nM. Steroid binding specificity was similar to that reported for leukocytes.
D000818:Animals; D001667:Binding, Competitive; D002645:Chickens / Q000097:blood*; D003907:Dexamethasone / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D003907:Dexamethasone