[Chromatographic method for quantitative determination of cordycepin in rat brain tissue after intraperitoneal injection of the antibiotic].
A procedure is developed for quantitative estimation of intraperitoneally administered unlabelled cordycepin in acid soluble pool isolated from rat brain tissue. The fraction containing 2'- and 3'-deoxyriboadenosine (cordycepin) in acid soluble pool of rat brain was isolated after consecutive chromatography on columns of Dowex 1 X 8, Dihydroxyboryl-SP 500 and Sephasorb-HP. At the same time, 2'-deoxyriboadenosine was isolated from the acid soluble pool fraction of control animals. Content of the antibiotic in brain tissue was estimated by its difference in these two fractions (control/experiment).
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002852:Chromatography, Ion Exchange / Q000379:methods; D003839:Deoxyadenosines / Q000008:administration & dosage / Q000302:isolation & purification / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D008297:Male; D051381:Rats
D003839:Deoxyadenosines
Khromatograficheskiĭ metod kolichestvennogo opredeleniia korditsepina v tkani mozga krys posle vnutribriushinnoĭ in"ektsii antibiotika.