title: Modulation of passive permeability by external ATP and cytoskeleton-attacking agents in cultured mammalian cells.
abstract: External ATP causes a passive permeability change in several transformed cells, but not in untransformed cells. We previously demonstrated that in CHO-K1 cells, a transformed clone of Chinese hamster ovary cells, the external ATP-dependent permeability change was induced when the intracellular ATP concentration was reduced by a mitochondrial inhibitor (Kitagawa, T. and Akamatsu, Y. (1981) Biochim. Biophys. Acta 649, 76-82). A permeability change with similar characteristics was also observed when the CHO cells were treated with external ATP and a cytoskeleton-attacking agent such as vinblastine or cytochalasin B. Just like mitochondrial inhibitors, vinblastine could increase the sensitivity of transformed 3T3 cells to external ATP but showed no effect on passive permeability of normal 3T3 cells. However, in contrast with the effect of the mitochondrial inhibitors, the cytoskeleton drugs caused the permeability change with little reduction of intracellular ATP concentration, suggesting different actions of these two kinds of drug on the permeability change. The present results suggest an important role of cytoskeletal structures in controlling the external ATP-dependent permeability change in transformed cells. Possible effects of intracellular ATP on cytoskeletal structures are also discussed.
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D003571:Cytochalasin B / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D010053:Ovary / Q000187:drug effects*; D014747:Vinblastine / Q000494:pharmacology*
chemical_list: D003571:Cytochalasin B; D014747:Vinblastine; D000255:Adenosine Triphosphate; D005947:Glucose