title: Characterization of rhesus macaque peripheral blood T-lymphocyte subpopulations.
abstract: Highly enriched rhesus macaque (Macaca mulatta) peripheral blood T-lymphocytes were separated into functional subpopulations by Fc-receptors. The T-lymphocyte population was comprised of both Fc-IgM (T mu +, 3.4 +/- 1.6) and Fc-IgG (T gamma +, 16.2 +/- 4.0) bearing cells. T-cells depleted of cells bearing Fc-IgG receptors (T gamma -) and T gamma + subpopulations were characterized and assessed for functional activity. T gamma + and T gamma - subpopulations were found to have the following characteristics: 1) T gamma + cells were stimulated by concanavalin-A (Con-A)3, pokeweed mitogen (PWM), and phytohemagglutinin-P (PHA-P), while T gamma - cells were stimulated by Con-A and PWM, but not PHA-P; 2) T gamma - cells were found to mediate PWM induced differentiation of autologous B-cells including EAC+ and EAC- enriched subpopulations, while T gamma + cells did not induce differentiation; 3) T gamma + cells released soluble factors which depressed mitogen stimulation of T gamma- cells; and 4) approximately 8-10% of the T gamma + cells phagocytized IgG sensitized bovine red blood cell (BRBC) immune complexes.
mesh_terms: D000818:Animals; D008251:Macaca / Q000276:immunology*; D008253:Macaca mulatta / Q000276:immunology*; D010587:Phagocytosis; D011961:Receptors, Fc / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology
chemical_list: D011961:Receptors, Fc