Differences in the Elution profiles of corticotropin-releasing activity in rat plasma and hypothalamic extracts following high performance liquid chromatography.
Extracts of rat plasma and hypothalami were fractionated by reversed-phase high performance liquid chromatography (HPLC) and the fractions were assayed in an in vitro bioassay for corticotropin-releasing activity. Corticotropin releasing factor (CRF) bioactivity was found in many fractions with retention times of 20-50 min. In contrast, material derived from the plasma of rats stressed by ether anaesthesia yielded only one peak of CRF bioactivity, with a retention time of 47-48 min. These results suggest that ACTH can be released from isolated pituitary cells by a number of components derived from HPLC of extracts of rat hypothalami. It is possible, however, that some of these are artefacts of the extraction process from post-mortem material. Because the CRF activity found in plasma was homogeneous by HPLC, plasma can be used to obtain physiologically active CRF.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003346:Corticotropin-Releasing Hormone / Q000032:analysis / Q000097:blood*; D004986:Ether; D005260:Female; D007031:Hypothalamus / Q000032:analysis*; D051381:Rats / Q000502:physiology*; D011919:Rats, Inbred Strains; D013312:Stress, Physiological / Q000097:blood / Q000139:chemically induced; D014020:Tissue Extracts / Q000032:analysis
D014020:Tissue Extracts; D004986:Ether; D003346:Corticotropin-Releasing Hormone