Classification of newly formed and migrating splenic lymphocytes.
Newly formed lymphoid cells in the spleens of normal young pigs were selectively labelled with 3H-TdR, using an extracorporeal perfusion system. One day later splenic and blood lymphocytes were separated by rosetting with sheep red blood cells for classical T cells and by a direct anti-globulin rosetting reaction for B cells. The proportion of newly formed lymphocytes in these subsets was determined by autoradiography. In this study we show that while both B and T lymphocytes are produced, B cells show a much higher incidence of newly formed cells. Labelled populations of spleen derived T and B lymphocytes were also found in the blood, but the B cells showed a lower labelling incidence than in the spleen.
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology; D002455:Cell Division; D002465:Cell Movement; D007958:Leukocyte Count; D008214:Lymphocytes / Q000145:classification*; D013154:Spleen / Q000166:cytology*; D013552:Swine; D013601:T-Lymphocytes / Q000502:physiology