No liquid holding recovery for chromosomal aberrations or sister-chromatid exchanges in irradiated G1 human lymphocytes.
We have tested G0 phase human peripheral lymphocytes for liquid holding recovery (LHR) mediated decreases in X-ray-induced chromosomal aberration yields and increases in sister-chromatid exchange (SCE) levels such as have been demonstrated for confluency-inhibited mouse cells in culture (Nagasawa and Little, 1981). No influence on either aberration yields or SCE levels was demonstrated. However, an effect at least superficially similar to the LHR effect was seen for rings and dicentrics, but not for deletions or SCE in lymphocytes in transition between G0 and G1 following PHA stimulation.
D002869:Chromosome Aberrations; D003434:Crossing Over, Genetic / Q000528:radiation effects*; D006801:Humans; D007399:Interphase; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects*; D010835:Phytohemagglutinins / Q000494:pharmacology; D012854:Sister Chromatid Exchange / Q000528:radiation effects*
D010835:Phytohemagglutinins