Characterization of oligosaccharides from the urine of loco-intoxicated sheep.
Two major oligosaccharides were isolated by preparative HPLC from the urine of a locoweed-fed sheep. Analysis by gas-liquid chromatography and mass-spectrometry indicated compositions of (Man)4(GlcNAc)2 and (Man)5(GlcNAc)2, respectively. Structures were determined by digestion with alpha-D-mannosidase and endo-beta-N-acetylglucosaminidases D and H, and comparison of the products by HPLC with synthetic standards, and oligosaccharides isolated from human mannosidosis urine. Incubation with an exo-beta-N-acetylglucosaminidase was without effect.
D000117:Acetylglucosamine / Q000032:analysis; D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D008358:Mannose / Q000032:analysis; D009844:Oligosaccharides / Q000652:urine*; D010939:Plant Poisoning / Q000652:urine / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000652:urine*
D009844:Oligosaccharides; D008358:Mannose; D000117:Acetylglucosamine