title: Metabolism of T-2 toxin in Curtobacterium sp. strain 114-2.
abstract: The metabolic pathway of T-2 toxin in Curtobacterium sp. strain 114, one of the T-2 toxin-assimilating soil bacteria, was investigated by thin-layer and gas-liquid chromatographic analyses. T-2 toxin added to the basal medium as a single carbon and energy source was biotransformed into HT-2 toxin and an unknown metabolite. Infrared, mass spectrum, proton magnetic resonance, and other physico-chemical analyses identified this new metabolite as T-2 triol. T-2 toxin was first deacetylated by the bacterium into HT-2 toxin, and this metabolite was then biotransformed into T-2 triol without formation of neosolaniol and T-2 tetraol. No trichothecenes remained in the culture medium after prolonged culture. Some properties of T-2 toxin-hydrolyzing enzymes were observed with whole cells, the cell-free soluble fraction, and the culture filtrate. Besides T-2 toxin, trichothecenes such as diacetoxyscirpenol, neosolaniol, nivalenol, and fusarenon-X were also assimilated by this bacterium.
mesh_terms: D001419:Bacteria / Q000201:enzymology / Q000378:metabolism*; D001711:Biotransformation; D007700:Kinetics; D012717:Sesquiterpenes / Q000378:metabolism*; D013605:T-2 Toxin / Q000031:analogs & derivatives / Q000378:metabolism*; D014255:Trichothecenes / Q000378:metabolism
chemical_list: D012717:Sesquiterpenes; D014255:Trichothecenes; C038854:scirpentriol; D013605:T-2 Toxin; C012351:HT-2 toxin