Soluble factors for B cell activation: a T cell hybridoma that secretes a specific B cell growth factor.
A murine T cell hybridoma, T91, has been established by fusion of BALB/c Con A-activated spleen cells with BW 5147 HAT-sensitive thymoma cells. T91 constitutively produced a factor that markedly enhanced the proliferative response to LPS of B cells cultured at low cell density. This factor is nonmitogenic by itself. It maintains in the absence of LPS, the proliferation of B cell blasts previously activated with LPS for 3 days. T91 supernatant contains neither IL 1 nor IL 2 nor TRF activity, and mixing experiments indicate the absence of factors suppressing these activities. The data suggest the existence of a specific B cell growth factor compatible with a two-signal model for B lymphocyte activation.
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D006825:Hybridomas / Q000032:analysis / Q000378:metabolism; D007375:Interleukin-1 / Q000032:analysis; D007376:Interleukin-2 / Q000032:analysis; D015848:Interleukin-5; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000032:analysis / Q000096:biosynthesis*; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D013601:T-Lymphocytes / Q000378:metabolism; D013945:Thymoma / Q000276:immunology
D007375:Interleukin-1; D007376:Interleukin-2; D015848:Interleukin-5; D008222:Lymphokines