title: Method of assay for 4-aminobutyrate-2-oxoglutarate aminotransferase.
abstract: In this paper an analytical colorimetric method is presented where 2,4-dinitrophenyl hydrazine is used to measure small amounts of succinic semialdehyde in the presence of 2-oxoglutarate. This method is applicable to the measurement of 4-aminobutyrate-2-oxoglutarate aminotransferase activity, where succinic semialdehyde formed during the enzymatic reaction has to be measured in the presence of 2-oxoglutarate.
mesh_terms: D000612:4-Aminobutyrate Transaminase / Q000032:analysis*; D000818:Animals; D003124:Colorimetry; D007656:Ketoglutaric Acids / Q000378:metabolism; D010659:Phenylhydrazines; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D007656:Ketoglutaric Acids; D010659:Phenylhydrazines; C004787:2,4-dinitrophenylhydrazine; D005680:gamma-Aminobutyric Acid; D000612:4-Aminobutyrate Transaminase; C009338:succinic semialdehyde