Lack of a requirement for idiotype matching: T cells from mice which cannot produce idiotype support idiotype-positive antibody response.
A requirement for idiotype matching was reported such that carrier-primed T helper cells from mice, which are unable to produce an idiotype (idiotype-negative) because of genetic reasons or adult anti-idiotype or neonatal anti-mu antibody treatment, did not provide helper function for an idiotype-positive antibody response. We have analyzed the requirement for idiotype matching in the response to phosphorylcholine by using mice which are idiotype-negative because they were injected with anti-idiotype antibody shortly after birth. Carrier-primed T cells from such animals supported idiotype-positive responses when mixed with normal or primed B cell populations and challenged with appropriate antigen; these responses were quantitatively and qualitatively similar to those obtained with T cells from idiotype-positive animals. These results demonstrate no requirement for idiotype matching and suggest that the procedure used to establish an idiotype-negative T cell donor may be decisive in showing a requirement for idiotype matching.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000008:administration & dosage / Q000096:biosynthesis*; D000921:Antibody-Producing Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D002452:Cell Count; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010767:Phosphorylcholine / Q000008:administration & dosage / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D007130:Immunoglobulin Idiotypes; D010767:Phosphorylcholine