Evidence for the presence of androgen receptors in purified rat Leydig cells.
Leydig cells, purified on two sequential Percoll gradients to purities of 89 +/- 1%, were used to study the binding of 17-beta-hydroxy-17 alpha-methyl-estra-4,9,11-triene-3-one(3H-R1881). The accumulation of 3H-R1881 in the nuclear fraction of these cells was time- and temperature-dependent. Specific binding was saturable with an apparent Ka of 0.14 nM-1 and a single class of binding sites at a concentration of 721 fmol/mg DNA. A fraction of the bound radioactivity in the nuclear pellet could be extracted with 0.4 M KCl, and a portion of this extracted steroid was associated with macromolecular species. The nuclear accumulation was androgen-specific. These data are consistent with the presence of androgen receptors in rat Leydig cells.
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D004054:Diethylstilbestrol / Q000378:metabolism; D004963:Estrenes / Q000378:metabolism*; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D015741:Metribolone; D051381:Rats; D011919:Rats, Inbred Strains; D011944:Receptors, Androgen / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism; D045165:Testosterone Congeners / Q000378:metabolism*; D014316:Tritium
D004963:Estrenes; D011944:Receptors, Androgen; D011987:Receptors, Steroid; D045165:Testosterone Congeners; D014316:Tritium; D015741:Metribolone; D013739:Testosterone; D004054:Diethylstilbestrol