Effect of an acute dose of ethanol on lipid peroxidation and on the activity of microsomal glutathione S-transferase in rat liver.
Acute ethanol administration (1.5 g/kg) to fasted rats resulted in a small but significant increase in the content of conjugated dienes in the microsomal fraction of liver. Treatment with 4-methylpyrazole prior to ethanol ingestion was able to reduce the ethanol-induced lipid peroxide formation (measured as conjugated dienes). No depletion of glutathione occurred within the first 2 hrs following ethanol administration by which time lipid peroxide formation is well established. The ethanol-induced inhibition of N-ethylmaleimide-stimulated microsomal glutathione S-transferase activity correlates positively to the concentration of conjugated dienes in the microsomal fraction of liver.
D000818:Animals; D000431:Ethanol / Q000097:blood / Q000494:pharmacology*; D000077604:Fomepizole; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D011720:Pyrazoles / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D008054:Lipid Peroxides; D011720:Pyrazoles; D000431:Ethanol; D000077604:Fomepizole; D005982:Glutathione Transferase; D005978:Glutathione