Enrichment of human epidermal Langerhans dendritic cells.
Langerhans cells (LC) were enriched from a human epidermal cell (EC) suspension by a rosette-forming technique. First EC were sensitized with the monoclonal antibody OKT6, and then they were mixed with ox erythrocytes (ORBC) coated with affinity-purified rabbit IgG anti-mouse IgG antibodies. Rosette-forming LC were then separated from non-rosetting EC by means of Percoll flotation. After hypotonic lysis of the ORBC, the cells obtained had a viability greater than 90%, and 67-98% of the viable cells were OKT6-positive. In functional studies the enriched cells were strongly stimulatory for allogeneic lymphocytes and were able to function as antigen-presenting cells for the T-cell response to the soluble antigen purified protein derivative.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002417:Cattle / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007801:Langerhans Cells / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology; D014374:Tuberculin Test
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G