Purification of reduced nicotinamide adenine dinucleotide by ion-exchange and high-performance liquid chromatography.
Combining ion-exchange (AG MP-1) and reversed-phase (C-18) partition chromatography accomplishes a higher degree of purification of NADH than either method can provide alone. Final elution in 95% ethanol, dehydration with anhydrous sodium sulfate, and storage in dry 1,2-propanediol over molecular sieves prevents hydrolysis of the purified dinucleotide.
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D009243:NAD / Q000302:isolation & purification*; D010084:Oxidation-Reduction; D011137:Polystyrenes
D011137:Polystyrenes; D009243:NAD; C003771:Amberlite XAD-2 resin