title: Exchange diffusion, electrodiffusion and rectification in the chloride transport pathway of frog skin.
abstract: Measurements of chloride flux ratios across frog skin at different clamping voltages showed that chloride transport at clamping voltages from 0 mV to and beyond the spontaneous potential is probably electrodiffusion. At reversed potentials a significant fraction of chloride transport could be described formally as exchange diffusion. Chloride conductance was found to be highly voltage dependent, being largest at hyperpolarizing clamping voltages. The transition from the less conducting state to the more conducting one was studied by recording the time course of the current after a step change in clamping voltage from 0 mV to hyperpolarizing voltages. The shape of the curve is sigmoidal, and the relative rate of change of current increases with increasing hyperpolarization. It is proposed that the change in conductance is governed by the same mechanism as in the toad skin, namely a change in chloride permeability due to voltage gating of chloride channels. The time course of transepithelial conductance after addition of amiloride to the outside solution indicates that a fraction of the decrease in conductance is due to closure of chloride channels caused by the change in intracellular potential due to the inhibition of the sodium channels.
mesh_terms: D000818:Animals; D001692:Biological Transport; D002712:Chlorides / Q000378:metabolism*; D003300:Copper / Q000494:pharmacology; D004058:Diffusion; D004594:Electrophysiology; D006207:Half-Life; D066298:In Vitro Techniques; D011893:Rana esculenta; D011896:Rana temporaria; D012867:Skin / Q000378:metabolism*; D013997:Time Factors
chemical_list: D002712:Chlorides; D003300:Copper