title: Ontogeny of surface markers on functionally distinct T cell subsets in the chicken.
abstract: Three subsets of chicken peripheral T cells (T1, T2 and T3) have been identified in peripheral blood of adult chickens on the basis of fluorescence intensity after staining with certain xenogeneic anti-thymus cell sera (from turkeys and rabbits). They differentiate between 3-10 weeks of age in parallel with development of responsiveness to the mitogens concanavalin A (Con A), phytohemagglutinin (PHA) and pokeweed mitogen (PWM). Functional tests on the T subsets, sorted with a fluorescence-activated cell sorter, have shown that T2, 3 cells respond to Con A, PHA and PWM and are capable of eliciting a graft-vs.-host reaction (GvHR). In contrast, although T1 cells respond to Con A, they respond poorly to PHA and not at all to PWM or in GvHR. There was some indication of cooperation between T1 and T2,3 cells for the PHA response. Parallels between these chicken subsets and helper and suppressor/cytotoxic subsets in mammalian systems are discussed.
mesh_terms: D000375:Aging; D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D002469:Cell Separation; D002642:Chick Embryo; D002645:Chickens; D003208:Concanavalin A / Q000276:immunology; D005434:Flow Cytometry; D006087:Graft vs Host Reaction; D007106:Immune Sera / Q000276:immunology; D007152:Immunologic Capping; D010835:Phytohemagglutinins / Q000276:immunology; D011043:Pokeweed Mitogens / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D007106:Immune Sera; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D003208:Concanavalin A