Binding of 5,9-dimethyl dibenzo[c,g]carbazole, a potent hepatocarcinogen, to mouse hepatic cytosolic proteins.
5,9-Dimethyl dibenzo[c,g]carbazole (5,9-diMe DBC) is a powerful organ-specific liver carcinogen in mice. Its binding to liver cytosolic proteins has been studied in vivo and in vitro with the aid of a tritium-labelled sample. Only two classes of proteins fractionated on Sephadex G100 appear to be involved in this binding: one class of high mol. wt. proteins (greater than 100 000) and another of around 45 000. The latter class contains all the glutathione-S-transferase activity (GST). The bound radioactivity has been determined after incubation in the presence or absence of microsomes. The in vivo binding has been investigated as a function of time. A highly significant covalent and noncovalent binding of 5,9-diMe DBC mostly to GST has been observed after metabolic activation.
D000818:Animals; D002227:Carbazoles / Q000378:metabolism*; D002273:Carcinogens / Q000378:metabolism; D002850:Chromatography, Gel; D003600:Cytosol / Q000378:metabolism*; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*
D002227:Carbazoles; D002273:Carcinogens; D011506:Proteins; C039387:5,9-dimethyldibenzo(c,g)carbazole; D005982:Glutathione Transferase