Investigations on the mechanism of photodynamic action of different psoralens with DNA.
Investigations on the photodynamic action of psoralens with DNA were performed, using experimental techniques of fluorescence lifetime and NMR-CIDNP, as well as SCF-MO and CNDO molecular orbital calculations. It has been shown that the formation of a biradical through the triplet state is the decisive step for psoralen dimer formation, as well as for cyclobutane addition with thymine, while singlet oxygen production is responsible for enzyme inactivation (e.g., lysozyme and trypsin). The molecular orbital calculations, in agreement with experimental results, indicate that the differences in biological effectivity of different psoralens are based on variations in triplet formation probability.
D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D011564:Furocoumarins / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008433:Mathematics; D010777:Photochemistry; D013050:Spectrometry, Fluorescence
D011564:Furocoumarins; D004247:DNA