Expression of HLA-like structures on a permanent human tumor line PC-93.
A permanent human tumor-line PC-93 was HLA allotyped with the use of a complement-dependent cytotoxicity test supplemented with absorption experiments. In comparison with the peripheral blood lymphocytes of the patient, whose adenocarcinoma of the prostate was the origin of PC-93, remarkable differences were observed. The tumor line lacked the expression of the genetically appropriate HLA-A1, A2 and B15 antigens. Instead of these antigens, cross-reactive HLA antigens seemed to be expressed on PC-93, i.e. All, A28 and B17. Absorption experiments showed that the antibodies reactive with the neo-HLA antigens were not the same as those reactive with the specific HLA antigens. This alien expression of HLA-like structures was specific for the patients' prostatic tumor line, as EB-virus transformed B-lymphocytes of the patient expressed the genetically appropriate HLA antigens.
D000042:Absorption; D000230:Adenocarcinoma / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D003429:Cross Reactions; D006680:HLA Antigens / Q000032:analysis* / Q000235:genetics; D015235:HLA-B Antigens; D015798:HLA-DR1 Antigen; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics / Q000276:immunology*
D006680:HLA Antigens; D015235:HLA-B Antigens; D015798:HLA-DR1 Antigen; D000949:Histocompatibility Antigens Class II