Inhibition of interleukin-2-induced proliferation of mouse T cells by normal mouse serum.
The effects of serum or plasma from both normal mice and nude mice on the proliferation of cultured T lymphocytes were studied. Normal mouse serum or heparinized plasma was found to significantly inhibit the proliferation of interleukin-2 (IL-2) stimulated cultured T cells, which were either autologous or allogeneic with the serum source. This inhibitory effect was either markedly or completely reversed by adding increased amounts of IL-2 to the cultures. Serum or plasma from nude mice was found to be as inhibitory to the proliferation as serum or plasma from normal mice. Absorption of the plasma with cultured T cells prior to assay did not eliminate or abrogate the plasma's inhibitory effect, but instead slightly increased its inhibitory activity. These studies indicate that serum or plasma from either normal mice or nude mice possesses a factor that can inhibit the proliferation of IL-2-stimulated cultured T cells. This factor probably acts either by interacting directly with the IL-2 or interfering with its stimulatory activity. The fact that adding excess IL-2 can reverse this effect may have important implications in the area of immune modulation and immunotherapy.
D000818:Animals; D001790:Blood Physiological Phenomena; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D007376:Interleukin-2 / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D013601:T-Lymphocytes / Q000187:drug effects*
D007376:Interleukin-2