Analysis of the neurohypophyseal components accumulating in the supraoptic nucleus of the rat after injection of colchicine.
[35S]Cysteine has been injected into the supraoptic nuclei of normal rats and of animals given 7 micrograms colchicine into the cerebrospinal fluid to inhibit transport of neurosecretory granules. Analysis of extracts of the supraoptic nuclei 20 min or 6 h after isotope injections showed that (i) colchicine does not affect biosynthesis, i.e., incorporation of tracer into the common precursors of neurohypophyseal hormones and their associated neurophysins, and (ii) processing of precursors inside the arrested granules proceeds normally.
D000818:Animals; D003078:Colchicine / Q000494:pharmacology*; D003545:Cysteine / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D009481:Neurophysins / Q000096:biosynthesis*; D010904:Pituitary Gland, Posterior / Q000187:drug effects / Q000502:physiology*; D010909:Pituitary Hormones, Posterior / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D013462:Sulfur Radioisotopes; D013495:Supraoptic Nucleus / Q000187:drug effects / Q000378:metabolism*
D009481:Neurophysins; D010909:Pituitary Hormones, Posterior; D013462:Sulfur Radioisotopes; D003545:Cysteine; D003078:Colchicine