title: Genome fusion mediated by the site specific DNA inversion system of bacteriophage P1.
abstract: The genome of bacteriophage P1 contains a segment which is invertible by site specific recombination between sequences near the outside ends of the inverted repeats which flank it. Immediately adjacent to this C segment is the coding sequence for cin, the enzyme catalyzing inversion. We show that multicopy plasmids carrying cin and the sequences at which it acts (cix) can form dimers in the absence of the host recA function. Further, such plasmids can be cotransduced with P1 markers at high frequency from recA lysogens, indicating cointegration with the P1 genome. It is thus demonstrated that a system whose primary role is the inversion of a specific DNA segment can also mediate intermolecular recombination.
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D007446:Chromosome Inversion; D003090:Coliphages / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D008957:Models, Genetic; D010957:Plasmids; D011938:Rec A Recombinases; D011995:Recombination, Genetic; D014161:Transduction, Genetic; D014764:Viral Proteins / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D004279:DNA, Viral; D014764:Viral Proteins; D011938:Rec A Recombinases