Increase in sensitivity of the microcytotoxicity assay by T cell growth factor (IL-2)-containing preparations.
Supernatants from Con A-stimulated rat spleen cell cultures containing TCGF were able to increase the sensitivity of the microcytotoxicity assay. Spleen cells from mice immunized with irradiated syngeneic tumour were precultivated for 24 h in a TCGF-containing medium. The precultivated immune spleen cells, when allowed to react with syngeneic tumour target cells, displayed a higher cytotoxic effect than the same immune spleen cells precultivated in a medium without TCGF. Similarly, the addition of TCGF-containing medium to the effector cells during the microcytotoxicity assay increased the cytotoxic effect. Combination of both schedules, i.e., precultivation of the immune effector cells in a TCGF-containing medium and addition of TCGF-containing medium to the effector cells during the microcytotoxicity assay produced the highest increase in sensitivity of the microcytotoxicity assay.
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology; D003208:Concanavalin A / Q000494:pharmacology; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D005260:Female; D007114:Immunization; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000276:immunology
D000951:Antigens, Neoplasm; D007376:Interleukin-2; D003208:Concanavalin A