title: B lymphocyte activation: entry into cell cycle is accompanied by decreased expression of IgD but not IgM.
abstract: To gain insight into the function of cell surface IgM (sIgM) and IgD (sIgD), the expression of these markers on B lymphocytes was quantitated during activation and progression through the cell cycle. Specifically, analysis and correlation of changes in cell cycle state, sIgM and sIgD expression and cell size following exposure of murine B cells to mitogenic levels of lipopolysaccharide and dextran sulfate is reported. As assessed by flow cytometric acridine orange analysis, a large proportion of normal splenic B cells respond within 48 h of exposure to these mitogens by entry into the cell cycle. This response is accompanied by an increase in cell size as determined by flow cytometric "time of flight" measurement. Flow cytometric immunofluorescence analysis reveals a simultaneous alteration in sIg expression. Specifically, cells leaving G0 and transiting G1 increase in diameter from 5 microns to 6 microns and lose greater than 80% of sIgD while sIgM remains constant. Progression through the remainder of the cell cycle is accompanied by a further increase in mean cell diameter to approximately 12 microns while sIgM and sIgD levels remain at G1 levels. The abrupt loss of sIgD as cells transit G1 suggests that an active process mediates this decrease.
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000502:physiology; D002453:Cell Cycle; D002478:Cells, Cultured; D005434:Flow Cytometry; D007072:Immunoglobulin D / Q000235:genetics*; D007075:Immunoglobulin M / Q000235:genetics*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D007072:Immunoglobulin D; D007075:Immunoglobulin M