Determinants of d-tubocurarine plasma protein binding in health and disease.
d-Tubocurarine protein binding was determined in plasma from normal surgical patients; patients with renal dysfunction; patients with alcoholic cirrhosis; and patients scheduled for coronary artery surgery and was compared with binding determined in solutions of isolated human serum albumin (HSA). The free fraction of d-tubocurarine was independent of plasma drug concentration throughout the range 0.1-3.0 micrograms/ml. The free fraction of d-tubocurarine in renal disease (0.44 +/- 0.05), liver disease (0.53 +/- 0.05), and cardiac disease (0.49 +/- 0.05) was not significantly different from that in normal patients (0.50 +/- 0.04). Furthermore, the free fraction of d-tubocurarine was unrelated to plasma concentrations of total protein, total globulin, albumin, total lipids, or alpha 1-acid glycoprotein in the four patient groups. The free fraction of d-tubocurarine (0.55 +/- 0.02, n = 7) in solutions of HSA (0.58 mM) was similar to that observed in plasma. Albumin, therefore, appears to be the major plasma binding protein for d-tubocurarine.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001798:Blood Proteins / Q000378:metabolism; D003327:Coronary Disease / Q000097:blood; D005260:Female; D006801:Humans; D007674:Kidney Diseases / Q000097:blood; D008104:Liver Cirrhosis, Alcoholic / Q000097:blood; D008297:Male; D008875:Middle Aged; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism; D013514:Surgical Procedures, Operative; D014403:Tubocurarine / Q000097:blood*
D001798:Blood Proteins; D012709:Serum Albumin; D014403:Tubocurarine