Purification of rat Schwann cells from cultures of peripheral nerve: an immunoselective method using surfaces coated with anti-immunoglobulin antibodies.
We report a method for deriving purified rat Schwann cells by immunoselective removal of fibroblasts. Contaminating fibroblasts labeled with antibody against specific surface marker Thy 1.1 are bound on plastic surfaces coated with a second antibody. The efficacy of the method is demonstrated by flow cytometry and by specific Schwann cell Ran-1 immunofluorescence.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000032:analysis; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000276:immunology; D005434:Flow Cytometry; D007074:Immunoglobulin G / Q000276:immunology; D051381:Rats; D012583:Schwann Cells / Q000166:cytology* / Q000276:immunology; D012584:Sciatic Nerve / Q000166:cytology* / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G