The effect of mitogenic lectins and monoclonal antibodies on intracellular free calcium concentration in human T-lymphocytes.
The effect of mitogenic lectins and the mitogenic antibody UCHT1 on the cytoplasmic free Ca2+ concentration ( [Ca2+]i) in human lymphocytes was investigated by using the fluorescent Ca2+-indicator quin2 . Phytohaemagglutinin, concanavalin A and UCHT1 increased [Ca2+]i in T-cells to a maximum level within 2 min. No T-cell response was seen with poke weed mitogen. None of the mitogens affected non-T-cell [Ca2+]i.
D000634:Aminoquinolines; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002118:Calcium / Q000378:metabolism*; D003208:Concanavalin A / Q000494:pharmacology; D005456:Fluorescent Dyes; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013601:T-Lymphocytes / Q000378:metabolism*
D000634:Aminoquinolines; D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D037102:Lectins; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D003208:Concanavalin A; C035738:Quin2-acetoxymethyl ester; D002118:Calcium