Translation of human interleukin 2 mRNA in Xenopus laevis oocytes.
Poly(A)-positive mRNA extracted from tonsillar mononuclear cells stimulated with phytohemagglutinin-M and 12-o-tetradecanoyl phorbol 13-acetate was successfully translated into biologically active interleukin 2 (IL-2) in Xenopus laevis oocytes, and secreted into the incubation medium. In control experiments, the extract of oocytes injected with either poly(A)-negative RNA or buffer did not show any IL-2 activity. By sucrose density gradient centrifugation analysis, IL-2 mRNA was found as a single peak corresponding to a sedimentation coefficient of 10-11S.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D008970:Molecular Weight; D009865:Oocytes / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D014066:Palatine Tonsil / Q000276:immunology; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D013601:T-Lymphocytes / Q000378:metabolism; D014982:Xenopus laevis / Q000235:genetics* / Q000378:metabolism
D007376:Interleukin-2; D012333:RNA, Messenger