In vitro sterilization of T lymphocyte lines infected with bacteria.
Rat T lymphocyte lines in culture contaminated with bacteria can be sterilized by a simple manoeuvre. The infected T cells are crudely separated from the bulk of contaminating organisms in an albumin density gradient. They are completely sterilized after overnight culture in medium containing 20% fresh serum from a conventionally kept adult rat in the presence of irradiated syngeneic adherent spleen cells.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000382:microbiology*; D007376:Interleukin-2 / Q000494:pharmacology; D010586:Phagocytes / Q000276:immunology; D051381:Rats; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000382:microbiology*
D007376:Interleukin-2