Neural crest-derived spinal and cranial sensory neurones are equally sensitive to NGF but differ in their response to tissue extracts.
The response of two distinct populations of neural crest-derived sensory neurones to nerve growth factor (NGF) and other neurotrophic activities present in extracts of chick tissues has been studied in vitro. Dorsal root ganglia (DRG) and the dorso-medial part of the trigeminal ganglion (DM-TG) from embryonic chicks of 6-11 days of incubation (E6-E11) were grown as either explant or dissociated neurone-enriched cultures. Over the age range studied NGF promoted survival and pronounced neurite outgrowth from both DRG and DM-TG neurones. Whilst extracts of chick eye, liver and spinal cord also elicited a marked response from E8 and older DRG neurones, DM-TG neurones were almost entirely unresponsive to the neurotrophic activity of these extracts.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D046508:Culture Techniques; D005123:Eye / Q000032:analysis; D005727:Ganglia, Spinal / Q000187:drug effects*; D008099:Liver / Q000032:analysis; D009414:Nerve Growth Factors / Q000032:analysis / Q000494:pharmacology*; D013116:Spinal Cord / Q000032:analysis; D012668:Trigeminal Ganglion / Q000187:drug effects*; D014276:Trigeminal Nerve / Q000187:drug effects*
D009414:Nerve Growth Factors