High-pressure liquid chromatographic separation of a mixture of corticosteroids, androgens, and progestins.
Fifteen steroids including corticosteroids, androgens, progestins, and their derivatives were completely separated by reverse-phase high-pressure liquid chromatography on a ChemcoPak 7 ODS-H column in 50 min. The elution procedures were first with water:methanol:acetonitrile:isopropanol 55:32:6.5:7.5 (v/v) for 15 min and followed with a linear gradient elution for 35 min from 0 to 80% of water:methanol:n-butanol 40:40:20 (v/v). The applicability of this method was successfully demonstrated in the analyses of the biological samples of carp plasma, testis, and head kidney.
D000305:Adrenal Cortex Hormones / Q000097:blood / Q000302:isolation & purification*; D000728:Androgens / Q000097:blood / Q000302:isolation & purification*; D000818:Animals; D002347:Carps; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007668:Kidney / Q000032:analysis; D008297:Male; D009928:Organ Specificity; D011372:Progestins / Q000097:blood / Q000302:isolation & purification*; D013329:Structure-Activity Relationship; D013737:Testis / Q000032:analysis
D000305:Adrenal Cortex Hormones; D000728:Androgens; D011372:Progestins