Expression of T-lymphoblast-encoded HLA-DR antigens on human T-B lymphoblast hybrids.
The mode of expression of novel HLA-DR antigens on hybrids of human T and B lymphoblastoid cell lines (LCL) was examined by several approaches. In each case, the results indicated that the novel antigens are T-LCL-encoded. First, hybrids of sublines of the T-LCL CEM with three different B-LCL express indistinguishable sets of novel HLA-DR antigens. Second, the novel HLA-DR and MT specificities of WI-L2 x HSB (a hybrid of a subline of the T-LCL HSB and the B-LCL WI-L2) match those of SB, a B-LCL derived from the same individual as HSB. Finally, an immunoselected variant of SB x CEM.1 (a hybrid of a subline of CEM and SB) lacking one copy of chromosome 6 and one of the hybrid's novel HLA-DR specificities also lacks a class I antigen known to be encoded by CEM.
D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D000949:Histocompatibility Antigens Class II; D006801:Humans; D006822:Hybrid Cells; D007106:Immune Sera; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D007106:Immune Sera