Hyperthermia in a differentiating murine erythroleukaemia cell line: II. Effect of heat on haem induction by radiation.
Friend erythroleukaemia cells (FELC) were induced to a haem-producing state by X-rays. The percentage of haem positive cells was maximum for doses between 10 and 15 Gy. Heat treatment at 42.0 degrees C or 45.0 degrees C during or after irradiation inhibited haem induction whereas heating before irradiation enhanced it. Incubation at 37 degrees C between heating and irradiation resulted in a decline in induction levels, indicating repair of heat damage that interacts with X-ray damage. Incubation at 37 degrees C between irradiation and heating did not result in changed haem induction levels, indicating a lack of repair of radiation damage that could interact with subsequent damage produced by heating.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D006418:Heme / Q000096:biosynthesis / Q000528:radiation effects*; D006358:Hot Temperature; D006979:Hyperthermia, Induced; D004915:Leukemia, Erythroblastic, Acute / Q000503:physiopathology*; D051379:Mice; D013696:Temperature
D006418:Heme