title: T-cell growth factor production and protein synthesis.
abstract: It is known that protein synthesis inhibitors (PSI) do not block production of some immunoregulatory and inflammatory mediators. Here we show that the production of T-cell growth factor (TCGF) by phytohaemagglutinin-stimulated human peripheral blood lymphocytes (PBL) was resistant to inhibition of protein synthesis more than 90-95%. Moreover, TCGF production was significantly accentuated in the presence of low concentrations of PSI in cultures from low- and high-producer donors. The presence of macrophages was non-essential for enhancement of TCGF production by PSI. When high doses of PSI were used, TCGF production was inhibited. In contrast, the synthesis of granulocyte/macrophage colony-stimulating factor (CSF) was blocked in the presence of low concentration of PSI. Hence, the results indicate that human TCGF produced by PBL belongs to a group of soluble factors whose production is relatively resistant to the action of PSI.
mesh_terms: D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000096:biosynthesis; D003513:Cycloheximide / Q000494:pharmacology; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D008214:Lymphocytes / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology
chemical_list: D003115:Colony-Stimulating Factors; D007376:Interleukin-2; D010835:Phytohemagglutinins; D011691:Puromycin; D003513:Cycloheximide