title: The infrastructure of aortic elastic fibers.
abstract: Elastic fibers are composed of a central core of elastin that is amorphous and electron-lucent in conventional transmission electron micrographs and peripheral microfibrils. A complex infrastructure within the amorphous elastin of mature rat aorta is made visible by fixation and staining with a glutaraldehyde-ruthenium red mixture in phosphate buffer or osmium-ruthenium red in cacodylate buffer. The infrastructure is composed of at least two interlacing but distinct elastic structural components; a framework of circumferentially orientated microfibrils and a three-dimensional meshwork of filaments that permeate the fiber. The latter resembles a reticulum that has previously been observed in freeze-fractured and negatively stained elastin and attributed to the supramolecular organization of elastin. Microfibrils also extend from the core of the elastic fiber into the surrounding matrix where they appear to function as anchoring fibers. These observations indicate that the elastic properties of the arterial wall are an integrated function of both elastin and microfibrils.
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000648:ultrastructure*; D004547:Elastic Tissue / Q000648:ultrastructure*; D004549:Elastin; D005404:Fixatives; D008854:Microscopy, Electron; D051381:Rats
chemical_list: D005404:Fixatives; D004549:Elastin