title: Direct visualization of unstained nucleic acids on a metal substrate.
abstract: Unstained DNA has been directly imaged for the first time on a metallic substrate. Reproducible, three-dimensional images of molecular morphology following aqueous deposition onto tungsten have been obtained. The images confirm the importance of reducing surface tension forces during drying. Preadsorption of poly-l-lysine seems to improve image quality although optimum conditions are quite different than those reported in the TEM literature for insulating substrates. Our results suggest that poly-l-lysine should be preadsorbed as a neutral molecule at high pH. Following adsorption, the pH should be reduced in order to establish a net positive surface charge density. The presence of positive charges on a tungsten surface helps to preserve DNA morphology following adsorption from aqueous solution.
mesh_terms: D000327:Adsorption; D004247:DNA; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D011061:Poly A; D011066:Poly C; D011068:Poly G; D011071:Poly T; D014054:Tomography / Q000379:methods; D014414:Tungsten
chemical_list: D011061:Poly A; D011071:Poly T; D011068:Poly G; C008961:poly G-poly C; C008950:poly A-T; D011066:Poly C; D004247:DNA; D014414:Tungsten