title: Protein production and processing in young adult and aged rat submandibular gland cells in vitro.
abstract: Protein production and processing were evaluated in vitro in dispersed submandibular gland cells from young adult (4-6 months) and aged (24 month) rats. A modest decrease (approx. 25%) in protein production (incorporation of radiolabeled amino acids into 10% CCl3COOH-insoluble material) was found with old cells in both continuous-pulse and pulse-chase studies. Also new protein processing, followed by gel electrophoresis and autofluorography of radiolabeled samples, showed specific, marked alterations in old cells. In particular a significant difference in the processing of a 225 000 molecular weight glycoprotein (likely the major rat submandibular mucin) was detected.
mesh_terms: D000375:Aging; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008854:Microscopy, Electron; D008970:Molecular Weight; D009077:Mucins / Q000378:metabolism; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D051381:Rats; D011919:Rats, Inbred Strains; D013363:Submandibular Gland / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D009077:Mucins