Structural and functional changes in epididymis following danazol plus testosterone enanthate administration in rabbit.
Danazol in combination with testosterone enanthate was administered to adult male rabbits to assess the structural and functional activity of epididymis. Seminal, biochemical and histological studies were made throughout the experiment. Sperm motility and count decreased markedly and at the end of treatment 75% of the animals became azoospermic. Seminal plasma GPC levels decreased significantly at 45 through 75 days of treatment. At 30 days of drug exposure, phospholipid, protein and sialic acid concentrations in caput epididymides were significantly low. Epididymal weight, phospholipid and protein contents markedly dropped in all segments of epididymides after 60 days of treatment. A reduction in epithelial and stereocilia height (caput and cauda), and lumen diameter (caput and corpus) was observed. Increase in intertubular stroma was evident. Lumen was devoid of spermatozoa at 60 days of treatment. All the changes were restored to normal 75 days after cessation of treatment. The combination therapy impaired the structural and functional integrity of the epididymis.
D000818:Animals; D003613:Danazol / Q000008:administration & dosage / Q000494:pharmacology*; D004822:Epididymis / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*; D005997:Glycerylphosphorylcholine / Q000032:analysis; D008297:Male; D019158:N-Acetylneuraminic Acid; D009929:Organ Size; D010743:Phospholipids / Q000032:analysis; D011245:Pregnadienes / Q000494:pharmacology*; D011506:Proteins / Q000032:analysis; D011817:Rabbits; D012794:Sialic Acids / Q000032:analysis; D013076:Sperm Count; D013079:Sperm Maturation / Q000187:drug effects; D013081:Sperm Motility / Q000187:drug effects; D013739:Testosterone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology
D010743:Phospholipids; D011245:Pregnadienes; D011506:Proteins; D012794:Sialic Acids; D013739:Testosterone; D005997:Glycerylphosphorylcholine; C004648:testosterone enanthate; D019158:N-Acetylneuraminic Acid; D003613:Danazol