Light reversal of carbon monoxide inhibition of 2-acetylaminofluorene N- and ring-hydroxylations by liver microsomes from 3-methylcholanthrene pretreated hamsters.
Light reversal of carbon monoxide inhibition of 2-acetylaminofluorene (AAF) N- and ring-hydroxylations is examined with liver microsomes from 3-methylcholanthrene (MC) pretreated hamsters. Photochemical action spectra indicate that carbon monoxide inhibition of ring-hydroxylation is readily reversed by light of 440 nm while that of N-hydroxylation is not reversed. These data suggest that these mixed function oxidations are catalyzed by CO combining enzymes with different properties.
D015073:2-Acetylaminofluorene / Q000378:metabolism*; D000818:Animals; D002248:Carbon Monoxide / Q000494:pharmacology*; D006224:Cricetinae; D008027:Light; D008297:Male; D008647:Mesocricetus; D008748:Methylcholanthrene / Q000494:pharmacology*; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors / Q000502:physiology* / Q000528:radiation effects
D008748:Methylcholanthrene; D002248:Carbon Monoxide; D015073:2-Acetylaminofluorene; D006899:Mixed Function Oxygenases