Monoclonal antibodies against group- and type-specific lipopolysaccharide antigens of Vibrio cholerae O:1.
Hybrid cell lines producing monoclonal antibodies against the O-antigenic determinants of Vibrio cholerae O:1 have been established. The specificity of the antibodies was ascertained by enzyme-linked immunosorbent assay inhibition experiments by using lipopolysaccharides from V. cholerae O:1 strains and type strains of groups O:2 and O:21. The anti-A antibody was of the immunoglobulin M (IgM) class, whereas the anti-B and -C antibodies were IgG3. The antibodies had a good agglutinating capacity when tested against V. cholerae O:1 strains in the slide agglutination test.
D000372:Agglutination Tests; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D005260:Female; D007136:Immunoglobulins / Q000032:analysis; D007525:Isoelectric Focusing; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014734:Vibrio cholerae / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007136:Immunoglobulins; D008070:Lipopolysaccharides