Effects of in vivo administration of steroids on release of 3H2O from [1 beta,2 beta-3H] and [1-3H]androstenedione by isolated rabbit ovarian follicles.
In vivo administration of progesterone and testosterone propionate to immature rabbits for 3 days resulted in a decreased ability of isolated ovarian follicles to generate 3H2O from [1 beta,2 beta-3H]androstenedione. By contrast, follicles isolated from rabbits treated with estradiol benzoate generated 3 times more 3H2O from the same substrate. Similar results were obtained when follicles were incubated with [1-3H]androstenedione. A similar pattern of differences was found when follicles from each group were analysed for estrogen content and when incubated with [4-14C]androstenedione and 14C-labelled estrogens isolated. These data suggest that estrogen may be a stimulant for its own production by rabbit ovarian follicles.
D000735:Androstenedione / Q000378:metabolism*; D000818:Animals; D001834:Body Water / Q000378:metabolism*; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D009929:Organ Size / Q000187:drug effects; D006080:Ovarian Follicle / Q000187:drug effects / Q000378:metabolism*; D010053:Ovary / Q000187:drug effects; D011374:Progesterone / Q000494:pharmacology*; D011817:Rabbits; D013739:Testosterone / Q000494:pharmacology*; D014316:Tritium; D014599:Uterus / Q000187:drug effects
D014316:Tritium; D013739:Testosterone; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D004054:Diethylstilbestrol