Preparation and properties of a hybrid toxin of modeccin A-chain and ricin B-chain.
Hybrid molecules were prepared from the A- and B-chains of the two toxic lectins ricin and modeccin by dialyzing mixtures of isolated chains to allow a disulfide bridge to be formed between them. Whereas the hybrid consisting of ricin A-chain and modeccin B-chain was non-toxic, the converse hybrid, modeccin A-chain/ricin B-chain, was even more toxic to Vero cells than were the parent toxins, native ricin and modeccin. A number of drugs (NH4Cl, monensin, trifluoperazine, verapamil, ionophore A23187) which protect cells against modeccin, but not against ricin, protected to some extent against the toxic hybrid, but less so than against native modeccin. The possibility is discussed that the modeccin A-chain of the hybrid may enter the cytosol by two routes, one which is highly efficient and identical to that used by native modeccin and another less efficient one which cannot be used by native modeccin.
D000643:Ammonium Chloride / Q000494:pharmacology; D004220:Disulfides; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000633:toxicity*; D008970:Molecular Weight; D008985:Monensin / Q000494:pharmacology; D037121:Plant Lectins; D014176:Protein Biosynthesis / Q000187:drug effects; D055503:Protein Multimerization; D054790:Ribosome Inactivating Proteins, Type 2; D012276:Ricin / Q000031:analogs & derivatives* / Q000633:toxicity
D004220:Disulfides; D037102:Lectins; D037121:Plant Lectins; D054790:Ribosome Inactivating Proteins, Type 2; C040213:modeccin A-chain-ricin B-chain hybrid toxin; D000643:Ammonium Chloride; D012276:Ricin; D008985:Monensin