Cortisol-free transcortin: preparation and effect on mitogen-stimulated lymphocytes.
Human cortisol-free transcortin was prepared from charcoal-treated serum. The major purification was achieved by affinity chromatography on an immunoadsorbent column of transcortin antibodies coupled to Sepharose 4B. A further purification on hydroxylapatite yielded pure transcortin with preserved steroid-binding activity. This preparation had no significant influence on the proliferation of human lymphocytes stimulated with phytohemagglutinin, nor did it increase the inhibition of lymphocyte proliferation by cortisol or dexamethasone.
D000328:Adult; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D006801:Humans; D006854:Hydrocortisone / Q000302:isolation & purification; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014156:Transcortin / Q000302:isolation & purification / Q000502:physiology*
D010835:Phytohemagglutinins; D014156:Transcortin; D013936:Thymidine; D006854:Hydrocortisone