[Enzyme activity of thiamine pyrophosphate in the rat after oxythiamine administration].
Intraperitoneal injection of hydroxythiamine to rats (1 mmol per kg bw) resulted after 2-4 h in a more than 4-fold decrease in the activity of the oxoglutarate dehydrogenase complex, pyruvate dehydrogenase complex and NADP-dependent isocitrate dehydrogenase in adrenal mitochondria. Inhibition of hyaloplasmic transketolase, 6-phosphogluconate dehydrogenase and NADP-dependent malate dehydrogenase occurred later. Based on the correlation of the time course of enzymatic activity in the adrenals and the decreased concentration of 11-hydroxycorticosteroids in the blood the paramount role in the maintenance of the steroidogenesis among thiamine pyrophosphate-containing enzymes is assigned to the oxoglutarate dehydrogenase and pyruvate dehydrogenase complexes.
D015062:11-Hydroxycorticosteroids / Q000097:blood; D000311:Adrenal Glands / Q000201:enzymology* / Q000378:metabolism; D000818:Animals; D003600:Cytosol / Q000201:enzymology; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D010119:Oxythiamine / Q000494:pharmacology*; D051381:Rats; D013379:Substrate Specificity; D013835:Thiamine Pyrophosphate / Q000378:metabolism*; D013844:Thiazoles / Q000494:pharmacology*
D015062:11-Hydroxycorticosteroids; D013844:Thiazoles; D010119:Oxythiamine; D009249:NADP; D010088:Oxidoreductases; D013835:Thiamine Pyrophosphate
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