Effects of ochratoxin A metabolites on yeast phenylalanyl-tRNA synthetase and on the growth and in vivo protein synthesis of hepatoma cells.
The ochratoxin A (OTA) metabolite (4R)-4-hydroxyochratoxin A [4R)-OTA) inhibits the aminoacylation of phenylalanine tRNA catalyzed by phenylalanyl-tRNA synthetase (PheRS) with a Ki-value of 0.9 mM as compared to 1.3 mM for OTA. It also inhibits protein synthesis and cell growth in the same manner as OTA. Ochratoxin alpha (OT alpha) does not affect either protein synthesis or cell growth.
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D009793:Ochratoxins / Q000378:metabolism* / Q000494:pharmacology; D010652:Phenylalanine-tRNA Ligase / Q000378:metabolism*; D014176:Protein Biosynthesis; D015003:Yeasts / Q000201:enzymology
D009793:Ochratoxins; C025589:ochratoxin A; D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase; C025650:4-hydroxyochratoxin A