title: Analysis of the water permeability of human granulocytes at subzero temperatures in the presence of extracellular ice.
abstract: The plasma membrane water permeability of human granulocytes in the presence of extracellular ice was determined experimentally on a cryomicroscope. Transient volumes of individual cells were measured at constant subzero temperatures subsequent to ice nucleation. Permeability values were deduced by adjustment of multiple parameters in a model to obtain an optimal fit to the data. The permeability was determined to be a function of both temperature and intracellular solute osmolality, with a reference value at 0 degrees C of 0.407 micrometers/atm.min and temperature and solute coefficients of 218kJ/mol and 1.09 Osm/kg.
mesh_terms: D002463:Cell Membrane Permeability; D005615:Freezing; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D008433:Mathematics; D008954:Models, Biological; D014867:Water / Q000378:metabolism
chemical_list: D014867:Water