title: Evidence for the presence of a hole in the capsid of turnip yellow mosaic virus after RNA release by freezing and thawing. Decapsidation of turnip yellow mosaic virus in vitro.
abstract: Turnip yellow mosaic virus (TYMV) RNA escapes from viral capsids after freezing and thawing the virus, and the remaining capsids look very similar to natural capsids in the electron microscope after negative staining [Katouzian-Safadi, M., Favre, A., and Haenni, A. L. (1980) Eur. J. Biochem. 112, 478-486]. In order to understand how an RNA of 2 X 10(6) Da (33% virus by weight) can escape from a compact protein shell we have compared artificial capsids formed after freezing TYMV and natural capsids produced in vivo in infected plants. We have used various physicochemical techniques including analytical ultracentrifugation, X-ray scattering, X-ray diffraction and orientation in a magnetic field. From the combination of these results we conclude that the escape of the RNA is accompanied by the formation of a hole in the capsid surface. The size of such a hole is estimated to 5-9 coat protein subunits.
mesh_terms: D002213:Capsid / Q000302:isolation & purification*; D005615:Freezing; D008854:Microscopy, Electron; D009029:Mosaic Viruses / Q000032:analysis*; D012367:RNA, Viral; D012542:Scattering, Radiation; D014461:Ultracentrifugation; D014961:X-Ray Diffraction
chemical_list: D012367:RNA, Viral