Effects of N-LAAM on [3H]etorphine binding in neuronal-enriched cell cultures.
Stereospecific [3H]etorphine binding sites are present in neuronal-enriched cell cultures dissociated from 7-day-old chick embryonic brain. Moreover, binding was regulated by both ions and GTP in a manner similar to that of in vivo brain tissue. When cultures were exposed to N-LAAM (10(-6) M) from day 6 to day 7 or 8 and assayed for binding at day 8, Bmax was decreased and KD was increased. These findings support our view that primary neuronal cultures are a suitable model with which to study interactions of drugs with opiate receptors.
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D005048:Etorphine / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D008691:Methadone / Q000031:analogs & derivatives*; D008692:Methadyl Acetate / Q000494:pharmacology*; D009019:Morphinans / Q000378:metabolism*; D009294:Narcotics / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D012964:Sodium / Q000494:pharmacology; D014316:Tritium
D009019:Morphinans; D009294:Narcotics; D014316:Tritium; D005048:Etorphine; D006160:Guanosine Triphosphate; D012964:Sodium; D008274:Magnesium; D008692:Methadyl Acetate; D008691:Methadone