Rapid determination of dopamine uptake in synaptosomal preparations.
A rapid method for assay of dopamine uptake in synaptosomal preparations involving reisolation of radiolabeled synaptosomes by rapid centrifugation is described. Utilizing this technique it was found that dopaminergic synaptosomes are relatively short lived, thus emphasizing the importance of rapid assay following tissue preparation. The effects of d-amphetamine, nomifensine and amfonelic acid were tested using the new procedure. The results of these experiments show a relative potency of uptake inhibition by these compounds which is comparable to previously published data.
D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D003913:Dextroamphetamine / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D007700:Kinetics; D009115:Muridae; D009268:Nalidixic Acid / Q000031:analogs & derivatives; D009287:Naphthyridines / Q000494:pharmacology; D009627:Nomifensine / Q000494:pharmacology; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*
D009287:Naphthyridines; D009627:Nomifensine; D009268:Nalidixic Acid; C006486:amfonelic acid; D003913:Dextroamphetamine; D004298:Dopamine