title: Occurrence of saxitoxin and other toxins in the liver of the pufferfish Takifugu pardalis.
abstract: Highly toxic livers of the pufferfish Takifugu pardalis were extracted with acidic ethanol. The toxins extracted were partially purified by chromatography on Bio-Gel P-2 and then Bio-Rex 70, resulting in separation into three fractions I, II and III. ratios of total mouse units per fraction were approximately 0.1:100:0.01, respectively, with tetrodotoxin (TTX) as standard. By TLC, electrophoresis and a TTX analyzer, Fr. II was identified as TTX and, unexpectedly, Fr. III as saxitoxin, while Fr. I remains unidentified.
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D004588:Electrophoresis, Cellulose Acetate; D005399:Fishes; D008099:Liver / Q000032:analysis*; D008387:Marine Toxins / Q000032:analysis*; D012530:Saxitoxin / Q000032:analysis*
chemical_list: D008387:Marine Toxins; D012530:Saxitoxin