Preparative high-performance liquid affinity chromatography.
The reactive triazine dye, Procion Blue MX-R, has been covalently attached to preparative-grade silica and used for the large-scale purification of rabbit muscle lactate dehydrogenase by high-performance liquid affinity chromatography (HPLAC). Purified dye was coupled directly to glycol-silylated silica via the reactive triazine ring to yield an adsorbent containing 12 mumol dye/g silica. Essentially homogeneous lactate dehydrogenase in 80% overall yield was obtained from crude extracts. Thus this report demonstrates the potential for adapting the speed of operation and resolution shown for triazine dye-HPLAC in analytical applications to preparative protein purification.
D000818:Animals; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification*; D009132:Muscles / Q000201:enzymology; D011817:Rabbits
D007770:L-Lactate Dehydrogenase