title: Effects of different injurious stimuli on cell death, proliferation, and collagen secretion by rabbit aortic smooth muscle cells and human umbilical vein endothelial cells in culture.
abstract: Rabbit aortic smooth muscle cells were exposed to hypoxia and the DMSO-soluble particulate matter of cigarette smoke (DSP) and the effects on the cells were compared to those of ultraviolet (UV) light, a wellknown cytotoxic stimulus. Hypoxia reduced cell proliferation and collagen secretion as long as it lasted (1, 3 and 24 hours), but these effects were reversible and no persistent toxic effects were seen for the next 48 hours, measured as leakage of prelabelled [3H]-thymidine from cells. DSP caused similar changes to those produced by UV-light. Proliferation and collagen secretion were reduced, and these effects were not reversible when the stimulus was removed after one hour. DSP was toxic as there was an increased leakage of [3H]-thymidine from cells. Increasing the concentration of DSP caused more pronounced effects while extending the incubation time to 3 hours did not, the latter being in contrast to the consequences of UV-irradiation. Endothelial cells were more sensitive to the effects of DSP than smooth muscle cells. DSP diluted 1:25 compared to the smallest concentration that had significant effects on smooth muscle cells reduced proliferation of endothelial cells, and in dilutions of 1:4 almost all of the cells detached. A possible role for DSP in the development of atherosclerosis is discussed.
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000502:physiology*; D002453:Cell Cycle; D002470:Cell Survival; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D004261:DNA Replication / Q000528:radiation effects; D004727:Endothelium / Q000502:physiology; D005260:Female; D006801:Humans; D000860:Hypoxia / Q000503:physiopathology; D009131:Muscle, Smooth, Vascular / Q000502:physiology* / Q000528:radiation effects; D011247:Pregnancy; D011817:Rabbits; D012906:Smoke / Q000009:adverse effects; D014466:Ultraviolet Rays; D014471:Umbilical Veins / Q000502:physiology*
chemical_list: D012906:Smoke; D003094:Collagen