Induction of benzylpenicilloyl specific antibodies, including IgE, by long-term administration of benzylpenicillin.
Using the ELISA and passive haemagglutination test we investigated the humoral immune response induced by long-term administration of benzylpenicillin in patients. After the second week of the course in twenty-four out of sixty-one patients, synthesis of benzylpenicilloyl (BPO) specific IgG, IgA, IgM and/or IgE could be demonstrated. BPO-specific IgE was always found in combination with BPO-specific IgG and/or IgM. No difference in the humoral immune response was found between patients who developed clinical hypersensitivity reactions and those who did not. In a follow-up study the disappearance of BPO-specific immunoglobulins of all classes was observed. Results of the epicutaneous tests showed that a long-term course of benzylpenicillin did not evoke delayed hypersensitivity. However intracutaneous tests with benzylpenicilloyl-polylysine or benzylpenicillin showed positive immediate type reactions. From the results it can be concluded that a BPO-specific IgE response is not necessarily associated with the development of a clinical hypersensitivity reaction.
D000917:Antibody Formation; D000918:Antibody Specificity; D004342:Drug Hypersensitivity / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005500:Follow-Up Studies; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis; D010400:Penicillin G / Q000008:administration & dosage / Q000276:immunology*; D012882:Skin Tests; D013997:Time Factors
D007074:Immunoglobulin G; D007073:Immunoglobulin E; D010400:Penicillin G