Identification of 3 beta-hydroxy-5,7-pregnadien-20-one and 3 beta-hydroxy-5,7-androstadien-17-one as endogenous steroids in the fetal horse gonad.
The 5,7-dienes, 3 beta-hydroxy-5,7-pregnadien-20-one and 3 beta-hydroxy-5,7-androstadien-17-one were extracted from fetal horse gonads and purified by solvent partition, thin-layer chromatography and high performance liquid chromatography. The isolated steroids were identified by comparison with the synthetic steroids using ultraviolet and mass spectroscopy and by gas chromatography-mass spectroscopy. The identification of these compounds as endogenous steroids, together with the data on their biosynthesis reported previously, support the proposal that in the fetal horse gonad there is a 5,7-diene pathway biosynthesizing 3 beta-hydroxy-5,7-androstadien-17-one, which is the proposed precursor for equilin in the placenta.
D000730:Androstadienes / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D004967:Estrogens / Q000302:isolation & purification* / Q000378:metabolism; D005260:Female; D005333:Fetus; D006736:Horses / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D010053:Ovary / Q000378:metabolism*; D011245:Pregnadienes / Q000302:isolation & purification* / Q000378:metabolism; D013737:Testis / Q000378:metabolism*
D000730:Androstadienes; D004967:Estrogens; D011245:Pregnadienes; C039560:3-hydroxyandrosta-5,7-dien-17-one; C037376:3-hydroxy-5,7-pregnadien-20-one