[Thrombopoiesis, thrombocyte count and thrombocyte function before and following cell separation].
About 50% of the circulating platelets can be taken from healthy donors by means of the cell separation technique. In order to investigate the effect of cell separation on thrombopoesis bone marrow samples were taken from 12 voluntary donors up to 5 times. The megacaryocytes were analyzed before and after cell separation by morphometric measures and determinations of platelet count and platelet function (adhesion, aggregation, spreading, retraction) were carried out. Removing of large amounts of platelets form the circulation by stimulation of thrombopoesis resulted in an increase of the peripherical platelet count already 48 h after cell separation. Of the platelet functions tested only aggregation induced by collagen was found to be increased after cell separation as compared to that before the procedure.
D001792:Blood Platelets / Q000166:cytology / Q000502:physiology*; D001854:Bone Marrow Cells; D002469:Cell Separation; D006003:Glycogen / Q000378:metabolism; D006410:Hematopoiesis; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology / Q000378:metabolism; D010973:Platelet Adhesiveness; D010974:Platelet Aggregation; D010976:Platelet Count
D006003:Glycogen
Thrombopoese, Thrombozytenzahl und Thrombozytenfunktion vor und nach Zellseparation.