Chromatin packing in normal and teratozoospermic human ejaculated spermatozoa.
Normal shaped (oval) and teratozoospermic spermatozoa have been compared by several techniques, including the use of "in vitro" S-S agents, cytofluorometry determination of DNA, total proteins, and ultracytochemical analysis of the presence and localization of SH proteins present in human sperm nuclei. A close relationship between S-S stability and chromatin packing is inferred from the differential sensitivity to S-S reduction agents exhibited by normal sperm nuclei which shows a lower Feulgen reactivity and special distribution of SH proteins compared to teratozoospermic cells. Both, S-S stability and chromatin packing are higher in normal sperms.
D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA / Q000378:metabolism; D004220:Disulfides / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008623:Mercaptoethanol / Q000494:pharmacology; D008854:Microscopy, Electron; D011506:Proteins / Q000378:metabolism; D013094:Spermatozoa / Q000002:abnormalities* / Q000648:ultrastructure
D002843:Chromatin; D004220:Disulfides; D011506:Proteins; D008623:Mercaptoethanol; D004247:DNA; D004229:Dithiothreitol