Modified immunoprecipitation procedure for the identification of human respiratory syncytial virus polypeptides.
When analysed by polyacrylamide gel electrophoresis, human respiratory syncytial virus harvested after a one step growth cycle and purified through a continuous sucrose density gradient was shown to be composed of nine structural proteins of 90, 68, 49, 42, 34, 28, 25, 19 an 13 kd. The 90, 49 and 19 kd polypeptides were identified as glycopolypeptides by glucosamine incorporation. A modified immunoprecipitation procedure confirmed the viral specificity of the 49, 42, 28, 25 and 19 kd polypeptides.
D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D006023:Glycoproteins / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D011092:Polyethylene Glycols; D011233:Precipitin Tests; D012136:Respiratory Syncytial Viruses / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis* / Q000302:isolation & purification; D015678:Viral Structural Proteins
D006023:Glycoproteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; D011092:Polyethylene Glycols