[Effect of the ionic strength of solutions on the size of ganglioside mycelia].
Gangliosides in aqueous media of low ionic strength (2-5 mM NaCl) and in concentrations over the critical ones (10(-5) M) form micellas which do not differ from liposomes as regards the chromatographic behavior on Sepharose 4R, with a molecular weight of greater than or equal to 10(7) dalton. In aqueous media of a higher ionic strength (greater than or equal to 20 mM NaCl), gangliosides form micellas which are eluted during chromatography in far later fractions than liposomes 70-80 nm in diameter, with a molecular weight of (1-5) X 10(5) dalton. It is assumed that the conclusions about ganglioside incorporation into the liposomal membrane made on the basis of their peaks coincidence are correct, provided that ganglioside-containing liposomes are obtained and chromatographed under high ionic strength (greater than or equal to 20 mM NaCl).
D000818:Animals; D002417:Cattle; D002847:Chromatography, Agarose; D005732:Gangliosides / Q000032:analysis*; D008081:Liposomes / Q000032:analysis; D008970:Molecular Weight; D009994:Osmolar Concentration
D005732:Gangliosides; D008081:Liposomes
Vliianie ionnoĭ sily rastvora na razmer gangliozidnykh mitsell.