Experiments with immunization of mice with blastocysts by an intrasplenic route.
A few attaching blastocysts from CBA/H mice were irradiated from a Cesium source and transferred into the spleen of male DBA2 mice. A booster immunization was performed after four weeks. Blood samples for preparation of antiserum to test for the presence of immunoglobulins directed against blastocyst surface determinants were obtained by a retro-orbital puncture. Specific antibodies were detected with a protein A-gold method, modified for transmission electron microscopy of air-dried blastocysts. The results showed that CBA/H blastocyst incubated in DBA2 immune serum were positive for protein A-gold labelling, while control blastocysts only possessed a few irregularly scattered gold particles. Thus, it seems as a deposition of antigens in the spleen tissue with persistence of the antigens at this site will result in detectable antibodies in the peripheral blood.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D001755:Blastocyst / Q000276:immunology* / Q000528:radiation effects; D006649:Histocompatibility Antigens / Q000276:immunology; D007117:Immunization, Secondary; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D013154:Spleen / Q000276:immunology*
D000906:Antibodies; D006649:Histocompatibility Antigens