Determination of endogeneous indoleacetic acid and tryptophol in mouse brain by high performance liquid chromatography with fluorometric detection.
A simple and sensitive method using high performance liquid chromatography with fluorometric detection has been developed for the identification and quantitation of the endogeneous tryptamine metabolites, indoleacetic acid (IAA) and tryptophol (TOL) in the normal mouse brain. The limits of sensitivity are 5pg for both IAA and TOL. The extract procedure from the brain is only to deproteinize samples. The mean concentrations of IAA and TOL in the mouse brain are 8.99 +/- 0.31 ng/g and 3.56 +/- 0.21 ng/g respectively. The effects of pargyline and tryptamine on the levels of IAA and TOL were also studied.
D000818:Animals; D001921:Brain / Q000187:drug effects; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D007210:Indoleacetic Acids / Q000032:analysis*; D007211:Indoles / Q000032:analysis*; D008297:Male; D051379:Mice; D010293:Pargyline / Q000494:pharmacology; D014363:Tryptamines / Q000494:pharmacology; D000438:Alcohols
D007210:Indoleacetic Acids; D007211:Indoles; D010293:Pargyline; D014363:Tryptamines; C030820:tryptamine; C005949:tryptophol; D000438:Alcohols; C030737:indoleacetic acid