[Radiation-induced binding of 2-amino-5,6-dihydro-1,3-4H-thiazine to DNA].
A study was made of the nature of radiation--induced bonds between DNA and 2-amino-5,6-dehydro-4H-thiazine (2-ADT) having a radioprotective action. Using the gelfiltration method and 35S-2-ADT, it was shown that the amount of the radioprotector bound to DNA increased with radiation dose and did not depend on the postirradiation treatment with 3 M LiCl or 3 M urea. No marked binding was noted after mixing the separately exposed DNA and the protector. It is concluded that a covalent linkage of DNA and 2-ADT occurs, upon irradiation, via short-living states of DNA and (or) the protector.
D003037:Cobalt Radioisotopes; D004247:DNA / Q000528:radiation effects*; D005720:Gamma Rays; D011837:Radiation-Protective Agents; D011854:Radiochemistry; D013843:Thiazines / Q000528:radiation effects*
D003037:Cobalt Radioisotopes; D011837:Radiation-Protective Agents; D013843:Thiazines; C018450:2-amino-5,6-dihydro-4H-1,3-thiazine; D004247:DNA
O radiatsionno indutsirovannom sviazyvanii 2-amino-5,6-digidro-1,3-4H-tiazina s DNK.