title: Efficiency of various bile salt preparations for stimulation of Clostridium difficile spore germination.
abstract: Taurocholate, desoxycholate, and cholate stimulated germination of Clostridium difficile spores in broth medium and enhanced recovery of C. difficile spores on a selective agar medium. Desoxycholate and some crude taurocholate preparations also inhibited multiplication of vegetative cells. At a concentration of 1.2 X 10(-2) M, sodium cholate inhibited multiplication of vegetative cells, but at concentrations of 1.2 X 10(-3) to 2.4 X 10(-3) M, it stimulated germination without inhibiting cell multiplication. Thus, pure sodium taurocholate and sodium cholate may effectively be incorporated in cefoxitin-cycloserine-fructose agar, whereas some crude preparations of sodium taurocholate decrease recovery on this medium.
mesh_terms: D001647:Bile Acids and Salts / Q000494:pharmacology*; D002793:Cholic Acids / Q000494:pharmacology; D003013:Clostridium / Q000187:drug effects / Q000502:physiology*; D003840:Deoxycholic Acid / Q000494:pharmacology; D013171:Spores, Bacterial / Q000187:drug effects; D013656:Taurocholic Acid / Q000494:pharmacology
chemical_list: D001647:Bile Acids and Salts; D002793:Cholic Acids; D003840:Deoxycholic Acid; D013656:Taurocholic Acid