Imprint immunofixation of IgG subclasses, using monoclonal subclass-specific antibodies.
Monoclonal mouse hybridoma antibodies to human IgG subclasses 1-4 were used in an imprint immunofixation (IIF) technique to characterize the IgG subclass of a panel of IgG myeloma and normal sera separated by thin-layer electrofocusing. The results show that the antisera were IgG subclass-specific by this technique and that IIF can be a useful method in identifying IgG subclasses of IgG myeloma proteins in whole sera.
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001345:Autoradiography; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000145:classification*; D007525:Isoelectric Focusing / Q000379:methods*; D009194:Myeloma Proteins / Q000276:immunology* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D009194:Myeloma Proteins