title: Localization of acetylcholinesterase and choline acetyltransferase in the rat pituitary gland.
abstract: Experiments were conducted to determine the presence of two cholinergic biomarkers, acetylcholinesterase (AChE) and choline acetyltransferase (ChAT) in the rat pituitary. A histochemical procedure for AChE was used to provide visualization of structures containing this enzyme. Radiochemical methods provided a sensitive assay for measuring ChAT activity. Nerve fibres staining for AChE activity were observed in the neurointermediate lobe, with the greatest concentrations appearing at the junction region with the pituitary stalk. Cells staining for AChE were found in the pars distalis and pars intermedia. ChAT activity correlated well with AChE distribution in pars nervosa and pars intermedia but not in pars distalis. The greatest levels of ChAT activity were in pars intermedia and the region where the stalk joins the pituitary. Significant values were also found for the pars nervosa. The presence of AChE and ChAT in pars intermedia and pars nervosa is evidence for a cholinergic innervation to these regions. In pars distalis, where other investigators have found muscarinic receptors, intense staining for AChE and absence of ChAT activity may indicate non-innervated, acetylcholine-sensitive sites.
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis*; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000032:analysis*; D002799:Cholinergic Fibers / Q000033:anatomy & histology; D006651:Histocytochemistry; D008297:Male; D010902:Pituitary Gland / Q000033:anatomy & histology / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase