A filtration double antibody radioimmunoassay that simplifies and semi-automates the isolation of immune precipitates.
A semi-automation of fluid phase double antibody radioimmunoassay has been developed. The immune precipitate that was formed in 96-well microtitration plates was harvested and washed on microfibre filters using a Titertek cell harvester. A disc transfer system originally designed for use with the harvester was used as a quick and easy method of transferring the filter discs containing immune precipitate into vials for counting. The results of radioimmunoassay using the microtitration plate-filtration and conventional tube-centrifugation method are essentially identical. The microtitration plate-filtration radioimmunoassay has the following advantages over the conventional tube-centrifugation method: (1) there is no centrifugation required; (2) handling of microtitration plate is easier than the tubes in racks; and (3) it requires much less time to perform the assay.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000937:Antigen-Antibody Reactions; D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods; D002498:Centrifugation; D011232:Chemical Precipitation; D005374:Filtration / Q000295:instrumentation* / Q000379:methods; D006041:Goats; D007130:Immunoglobulin Idiotypes / Q000302:isolation & purification; D010795:Phthalic Acids / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay / Q000295:instrumentation* / Q000379:methods
D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D010795:Phthalic Acids; C032279:phthalic acid