Phototrophic growth of halobacteria and its use for isolation of photosynthetically-deficient mutants.
Phototrophic growth conditions for halobacteria in complex and synthetic media have been established, demonstrating the photosynthetic capacity of this class of archaebacteria. Mutagenesis, 5-bromo-2'-deoxyuridine selection and screening techniques are described which are useful tools in the elucidation of the structure-function relationship of retinal proteins in halobacteria.
D000693:Anaerobiosis; D001436:Bacteriorhodopsins / Q000096:biosynthesis; D001973:Bromodeoxyuridine / Q000494:pharmacology; D003470:Culture Media; D006217:Halobacterium / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D008027:Light; D009154:Mutation; D010788:Photosynthesis; D012172:Retinaldehyde / Q000096:biosynthesis
D003470:Culture Media; D001436:Bacteriorhodopsins; C017827:bacterio-opsin; D001973:Bromodeoxyuridine; D012172:Retinaldehyde