Adrenaline: occurrence in the bovine retina.
Using high performance liquid chromatography (HPLC) and electrochemical detection, a substance corresponding to adrenaline was identified in crude perchloric acid extracts from bovine retina. The substance did not originate from exogenous contamination such as blood. Retinal tissues also contained phenylethanolamine-N-methyl-transferase (PNMT) activity and the retinal enzyme had a similar substrate specificity as purified bovine PNMT. The pigment epithelium/choroid complex also had PNMT activity and an analysis of extracts by HPLC revealed the presence of small amounts of adrenaline. The results suggest that adrenaline is of functional importance in the bovine retina.
D000818:Animals; D002417:Cattle; D002829:Choroid / Q000032:analysis / Q000201:enzymology; D004298:Dopamine / Q000032:analysis; D004837:Epinephrine / Q000032:analysis*; D009638:Norepinephrine / Q000032:analysis; D010625:Phenylethanolamine N-Methyltransferase / Q000378:metabolism; D010857:Pigment Epithelium of Eye / Q000032:analysis / Q000201:enzymology; D012160:Retina / Q000032:analysis* / Q000201:enzymology
D010625:Phenylethanolamine N-Methyltransferase; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine