[Cytochemical characteristics of the clones of human bone marrow stromal fibroblasts].
Cytochemical methods were used to study the activity of acid and alkaline phosphatases, neutral and acid nonspecific esterases, and peroxidase in the clones of stromal human marrow stromal fibroblasts at the 10th-12th day of cultivation with rabbit feeder. All the cells in the clones contained acid phosphatase. All the colonies had 5 to 90% of cells with positive response to alkaline phosphatase. The colony cells did not show any neutral and acid nonspecific esterases or peroxidase.
D000469:Alkaline Phosphatase / Q000032:analysis*; D002454:Cell Differentiation; D003114:Colony-Forming Units Assay; D046508:Culture Techniques; D005347:Fibroblasts / Q000166:cytology* / Q000201:enzymology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000201:enzymology; D006801:Humans
D000469:Alkaline Phosphatase
Tsitokhimicheskaia kharakteristika klonov stromal'nykh fibroblastov kostnogo mozga cheloveka.