[Protective antigens of the vaccinia virus].
The comparison of the polypeptide composition of 3 vaccinia virus strains, L-IVP, B-51 and CM-63, has revealed that strains L-IVP and B-51 are similar in their polypeptide composition, while in strain CM-63 capsid polypeptide with a molecular weight of 34000 daltons is absent or has altered electrophoretic mobility. As the result of the isolation of vaccinia envelopes (from strain L-IVP) and the electrophoretic separation of their polypeptides in plates with polyacrylamide gel 10 polypeptides have been obtained in 7 fractions, each containing 1 or 2 polypeptides. The immunization of rabbits with individual fractions has demonstrated that the formation of virus-neutralizing antibodies is induced mainly by 4-5 polypeptides in 3 fractions, having the highest molecular weight (54000-31000 daltons) and constituting about 19% of all proteins in the whole virion. The low-molecular envelopes polypeptides have been found to play no essential role in inducing the formation of virus-neutralizing antibodies. The highest antibody titers (1: 15625) have been detected in antisera to the preparations of whole vaccinia virus envelopes.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis* / Q000302:isolation & purification; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D007114:Immunization; D008970:Molecular Weight; D009500:Neutralization Tests; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification; D011817:Rabbits; D014616:Vaccinia virus / Q000276:immunology*; D014771:Virion / Q000276:immunology; D014776:Virus Cultivation
D000956:Antigens, Viral; D010455:Peptides
Izuchenie protektivnykh antigenov virusa vaktsiny.