[Effects of rectal administration of an ampicillin suppository (KS-R1) on cecal microflora and cecal weight in mice].
KS-R1 is a new rectal preparation of ampicillin (ABPC) sodium. Changes of cecal microflora, changes of cecal weight and ABPC concentrations in the cecum after rectal administration of KS-R1 to mice were compared with those after oral and subcutaneous administration of ABPC. When KS-R1 was administered to mice by 25.0 mg/kg 3 times a day for 10 days continuously, a little changes were observed in the number of Enterobacteriaceae, Staphylococcus and Lactobacillus in the cecum. However, at the fifth day after completing the rectal administrations, normal level of microflora was gained. This change of microflora closely resemble those in case when ABPC sodium was administered subcutaneously. On the other hand, when ABPC was administered orally, changes of microflora were large, i.e. the number of Enterobacteriaceae and Streptococcus increased remarkably, the number of Fusobacterium increased and the number of Staphylococcus and Bacteroides decreased. Recovery of normal level of microflora was late. The cecal weight did not show changes but the increase observed in the case when ABPC was orally administered. This increase was due to the increase of moisture in the cecum. The concentrations of ABPC in the cecum when administered by 25.0 mg/kg were in the order of oral administration greater than rectal administration (KS-R1) greater than subcutaneous administration. The highest concentrations were respectively 219.6, 54.1 and 5.1 micrograms/g. Changes of microflora in the cecum was great in proportion to the concentrations of ABPC.(ABSTRACT TRUNCATED AT 250 WORDS)
D000284:Administration, Oral; D000667:Ampicillin / Q000008:administration & dosage* / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002432:Cecum / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology; D004755:Enterobacteriaceae / Q000302:isolation & purification; D007273:Injections, Intramuscular; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D013291:Streptococcus / Q000302:isolation & purification; D013488:Suppositories
D013488:Suppositories; D000667:Ampicillin