Elemental changes at irritant reactions due to chromate and nickel in guinea-pig epidermis.
The present investigation was performed to examine the possibility of using energy dispersive X-ray microanalysis (EDX) to obtain information on epidermal reactions at irritant dermatitis. The elemental redistribution in the stratum germinativum and stratum spinosum of the guinea-pig epidermis after intradermal injection of a chromate and a nickel solution was studied in thick cryo-sections. The two solutions caused different changes in elemental content in both cell layers. Nickel sulfate caused a conspicuous decrease in K levels whereas Na and Cl were elevated in both cell layers. Potassium chromate caused an increase in Cl but lowered the P values. The result of this study show that it is possible to use EDX-analysis of thick skin sections to obtain information on physiologically relevant alterations in epidermis at irritant dermatitis.
D000818:Animals; D002840:Chromates / Q000633:toxicity*; D004577:Electron Probe Microanalysis; D004602:Elements / Q000032:analysis*; D006168:Guinea Pigs; D007509:Irritants / Q000633:toxicity*; D009532:Nickel / Q000633:toxicity*; D017680:Potassium Compounds; D012867:Skin / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure*
D002840:Chromates; D004602:Elements; D007509:Irritants; D017680:Potassium Compounds; C029938:nickel sulfate; C027373:potassium chromate(VI); D009532:Nickel