Analysis of porphyrin precursors, 5-aminolevulinic acid derivatives by isotachophoresis.
Porphyrin precursors, 5-aminolevulinic acid (ALA), 4,5-dioxovaleric acid (DOVA), and porphobilinogen (PBG) can be simultaneously and conveniently analyzed by isotachophoresis. The quantitative analysis requires a minimum of 12 nmol for ALA, 4 nmol for DOVA, and 35 nmol for PBG. The reproducibility determined as coefficient of variation for qualitative and quantitative determination is within 4 and 5%, respectively. Isotachophoresis permits simple, efficient and quantitative detection, and identification of ALA derivatives in a mixture.
D000622:Aminolevulinic Acid / Q000031:analogs & derivatives / Q000032:analysis*; D002708:Chlorella / Q000201:enzymology; D003124:Colorimetry / Q000379:methods; D004586:Electrophoresis / Q000379:methods; D007982:Levulinic Acids / Q000032:analysis*; D011162:Porphobilinogen / Q000032:analysis; D011166:Porphyrins / Q000032:analysis*; D000637:Transaminases; D014631:Valerates / Q000032:analysis
D007982:Levulinic Acids; D011166:Porphyrins; D014631:Valerates; C007509:4,5-dioxovaleric acid; D011162:Porphobilinogen; D000622:Aminolevulinic Acid; D000637:Transaminases; C019687:aminolevulinate aminotransferase