Alpha-naphthyl butyrate esterase (a selective cytochemical monocyte marker).
This paper describes a cytochemical technique for alpha-naphthyl butyrate esterase demonstration in monocytes obtained from peripheral blood of normal subjects, of a patient with reactive monocytosis and of a patient with myelomonocytic leukemia. It utilizes pH 6.1 alpha-naphthyl butyrate as substrate. At this pH value, alpha-naphthyl butyrate is a specific monocyte marker. The cytochemical procedure proposed is simple, rapid and standardized, as it utilizes stable and easily available reagents.
D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D006651:Histocytochemistry; D006801:Humans; D008722:Methods; D009000:Monocytes / Q000201:enzymology*
D002265:Carboxylic Ester Hydrolases; C021855:naphthylbutyrate esterase