Analysis of the water permeability of human granulocytes at subzero temperatures in the presence of extracellular ice.
The plasma membrane water permeability of human granulocytes in the presence of extracellular ice was determined experimentally on a cryomicroscope. Transient volumes of individual cells were measured at constant subzero temperatures subsequent to ice nucleation. Permeability values were deduced by adjustment of multiple parameters in a model to obtain an optimal fit to the data. The permeability was determined to be a function of both temperature and intracellular solute osmolality, with a reference value at 0 degrees C of 0.407 micrometers/atm.min and temperature and solute coefficients of 218kJ/mol and 1.09 Osm/kg.
D002463:Cell Membrane Permeability; D005615:Freezing; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D008433:Mathematics; D008954:Models, Biological; D014867:Water / Q000378:metabolism
D014867:Water