Individual molecular species of phosphatidylcholines and phosphatidylinositols in liver of rats fed bis(2-ethylhexyl)phthalate.
Rats were given a diet containing 1% bis(2-ethylhexyl)phthalate (DEHP) for 3 weeks, and their hepatic lipids analyzed. Phosphatidylcholines increased by 20%, while other phospholipid classes and cholesterol remained unchanged and triglycerides fell. The composition of molecular species of phosphatidylcholines was changed. Thus, the hepatic content of the major species, 1-palmitoyl-2-oleoyl-, 1-palmitoyl-2-arachidonoyl- and 1-stearoyl-2-arachidonoylphosphatidylcholines, rose by about 150%, 90% and 70%, respectively. The content of the other major species, 1-palmitoyl-2-linoleoyl- and 1-stearoyl-2-linoleoylphosphatidylcholine fell by about 20% and 30%, respectively. The content of alkyl-acyl analogues of phosphatidylcholines increased by about 70%, but the composition of molecular species remained the same. The composition of molecular species of phosphatidylinositols was also unchanged. Thus, the analyses show that DEHP can induce selective changes in molecular species of certain phospholipids in the liver. This could be important for the functioning of membrane structures in the hepatocyte.
D000818:Animals; D004051:Diethylhexyl Phthalate / Q000494:pharmacology*; D005260:Female; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D010716:Phosphatidylinositols / Q000096:biosynthesis*; D010743:Phospholipids / Q000096:biosynthesis / Q000302:isolation & purification; D010795:Phthalic Acids / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D010743:Phospholipids; D010795:Phthalic Acids; D004051:Diethylhexyl Phthalate