Uncoupling protein in human brown adipose tissue mitochondria. Isolation and detection by specific antiserum.
A protein of Mr 32 000 has been isolated from human infant brown adipose tissue mitochondria following the procedure used to purify the uncoupling protein from rat brown adipose tissue mitochondria. A specific antiserum has been raised against the human 32 kDa protein, and used to detect it by probing mitochondrial proteins separated by SDS-PAGE. The protein is present in large amounts in brown adipose tissue but is undetectable in human liver, heart or white adipose tissue. It has strong immunological cross-reactivity with rat brown adipose tissue uncoupling protein.
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D000918:Antibody Specificity; D002352:Carrier Proteins; D055598:Chemical Phenomena; D002621:Chemistry; D005260:Female; D006801:Humans; D007106:Immune Sera; D066298:In Vitro Techniques; D007223:Infant; D007231:Infant, Newborn; D007473:Ion Channels; D008297:Male; D008565:Membrane Proteins / Q000302:isolation & purification*; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins; D008970:Molecular Weight; D011863:Radioimmunoassay; D051381:Rats; D014475:Uncoupling Agents / Q000302:isolation & purification; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D007106:Immune Sera; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D014475:Uncoupling Agents; D000071256:Uncoupling Protein 1