title: Rat atrial natriuretic factor. Purification and vasorelaxant activity.
abstract: The atrial natriuretic activity of rat heart has been found to exist in multiple forms. One of these factors has been purified to apparent homogeneity by a combination of gel filtration and high pressure liquid chromatography in two different systems and its amino acid composition determined. The purified active peptide is shown to have a molecular weight of approximately 3800. In addition, the vasorelaxant activity of rat atrium has been purified and found to co-chromatograph with the natriuretic activity in all chromatographic systems employed. Thus, the vasorelaxant activity resides in the natriuretic factor. The existence of this new multifunctional peptide implies a higher level of complexity for cardiovascular control of blood volume and pressure.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D009320:Atrial Natriuretic Factor; D001681:Biological Assay; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006325:Heart Atria / Q000032:analysis; D008970:Molecular Weight; D009206:Myocardium / Q000032:analysis*; D009318:Natriuresis; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D014665:Vasodilator Agents / Q000302:isolation & purification*
chemical_list: D000596:Amino Acids; D014665:Vasodilator Agents; C472610:atrial natriuretic peptide, rat; D009320:Atrial Natriuretic Factor