title: The effect of anti-oxidants and medium composition on isolation and culture of alveolar type II pneumocytes.
abstract: Alveolar type II pneumocytes were isolated from adult male rabbits and were placed in primary culture. The presence of anti-oxidants throughout the isolation procedure, particularly ascorbic acid and glutathione, was found to enhance in vitro attachment efficiency of cells. The use of a culture medium substituting D-valine for L-valine also significantly enhanced attachment efficiency. Although these cells do not ordinarily proliferate in culture, a low-serum medium containing insulin, transferrin, selenium and hydrocortisone allowed limited proliferation in addition to promoting dome formation in culture.
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D005978:Glutathione / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology; D008297:Male; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011817:Rabbits
chemical_list: D000975:Antioxidants; D003470:Culture Media; D005978:Glutathione; D001205:Ascorbic Acid; D006854:Hydrocortisone