[Analysis of radiation injuries of DNA from liver and transplanted tumors in vitro].
A comparative study of some radiation effects on DNAs from mouse liver, Ehrlich ascites tumor and hepatoma 22A was made after X-irradiation of the 0.02% aerated solutions in 0.1 M NaCl. The electrophoretic and spectrophotometric methods were used to measure the radiation-chemical yields of single- and double-strand breaks, DNA secondary structure defects, a malonaldehyde analogue and pyrimidine hydroperoxides. DNA from tumors was found to be more affected by radiation than DNA from normal liver: this may be due to a higher degree of base damage (the yield of hydroperoxides) and to transformation of original secondary structure defects into single-strand breaks at low irradiation doses.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis*; D004247:DNA / Q000032:analysis / Q000528:radiation effects*; D004273:DNA, Neoplasm / Q000032:analysis / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000032:analysis; D004307:Dose-Response Relationship, Radiation; D066298:In Vitro Techniques; D008099:Liver / Q000032:analysis*; D008114:Liver Neoplasms, Experimental / Q000032:analysis*; D051379:Mice; D011854:Radiochemistry
D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D004247:DNA
Analiz radiatsionnogo povrezhdeniia in vitro DNK pecheni i perevivaemykh opukholeĭ.