Extraction and analysis of chlorpromazine and its major metabolites in post mortem material by enzymic digestion and HPLC.
A method is described for the determination of chlorpromazine and some of its major metabolites in post mortem specimens by enzymic digestion of the tissues with ethyl acetate using a simple, single micro-extraction method, followed by HPLC of the extracts using a 10 micron silica column packing and a mobile phase consisting of ethanolamine:methanol:water. Separation and quantitation of 7-hydroxy-chlorpromazine, chlorpromazine, chlorpromazine sulfoxide, norchlorpromazine and norchlorpromazine sulfoxide was achieved employing mesoridazine as an internal standard.
D002746:Chlorpromazine / Q000032:analysis* / Q000506:poisoning; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D006801:Humans; D008099:Liver / Q000032:analysis; D013270:Stomach / Q000032:analysis
D002746:Chlorpromazine