Termination of tritiated thymidine incorporation by freezing the cells.
It is often desirable to be able to stop the uptake of tritiated thymidine by a number of transforming lymphocyte cultures at a specific time. This is important for kinetic studies but may be difficult if the number of cultures to be harvested at one time is large. We describe in this report a simple method to stop the incorporation of tritiated thymidine into any number of transforming lymphocyte cultures simultaneously.
D005615:Freezing; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium
D014316:Tritium; D013936:Thymidine