Identification of thyroglobulin mRNA sequences in the nucleus and the cytoplasm of cultured thyroid cells: a post-transcriptional effect of thyrotropin.
Cytoplasmic and nuclear RNA were isolated from porcine thyroid cells cultured with or without thyrotropin. After denaturation by glyoxal-DMSO treatment, the RNAs were analyzed on agarose gels, transferred onto nitrocellulose filters and hybridized with a [32P]-labelled thyroglobulin cDNA clone. In the cytoplasmic RNA a single mature thyroglobulin mRNA molecule (9 Kb) was present whereas in the nuclear RNA several molecular species (24 to 3.3 Kb) were identified. In the nucleus only the 9 Kb mRNA was polyadenylated. In cytoplasmic preparations the level of mature thyroglobulin mRNA was two-fold higher when cells were cultured with thyrotropin. In nuclear preparations only the level of the mature transcript was affected by the presence of thyrotropin, increasing relative to the concentration of the higher molecular weight species. These results suggest a hormonal effect at the post-transcriptional level that may serve to stabilize the mature mRNA molecule.
D000818:Animals; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005786:Gene Expression Regulation; D011061:Poly A / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013552:Swine; D013954:Thyroglobulin / Q000235:genetics*; D013961:Thyroid Gland / Q000502:physiology*; D013972:Thyrotropin / Q000494:pharmacology*
D012333:RNA, Messenger; D011061:Poly A; D013972:Thyrotropin; D013954:Thyroglobulin