[Mechanism of action of diuretics studied by a fluorescent probe method].
The mercuric-xanthine diuretic novurit, ethacrynic acid and furosemide reduced the fluorescence of 1,8-anilinonaphthalene-8-sulfonate (1,8-ANS) in a suspension of phospholipid liposomes and human serum albumin solution. Ethacrynic acid and furosemide eliminated the fluorescence of 1,8-ANS in a suspension of non-energized mitochondria of rat kidney, while novurit provoked fluorescence intensification. The constants of novurit and ethacrynic acid binding with kidney mitochondria determined by the degree of changes in 1,8-ANS fluorescence variation appeared significantly higher than the constants of binding with phospholipids and serum albumin. However furosemide did not show a predominant binding with kidney mitochondria.
D000479:Alkylmercury Compounds / Q000378:metabolism; D000817:Anilino Naphthalenesulfonates / Q000378:metabolism; D000818:Animals; D004232:Diuretics / Q000378:metabolism*; D004338:Drug Combinations / Q000378:metabolism; D004976:Ethacrynic Acid / Q000378:metabolism; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000378:metabolism*; D005665:Furosemide / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000378:metabolism; D008722:Methods; D008928:Mitochondria / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D012709:Serum Albumin / Q000378:metabolism; D013806:Theophylline / Q000378:metabolism
D000479:Alkylmercury Compounds; D000817:Anilino Naphthalenesulfonates; D004232:Diuretics; D004338:Drug Combinations; D005456:Fluorescent Dyes; D012709:Serum Albumin; C021054:mercurophylline; D005665:Furosemide; D013806:Theophylline; D004976:Ethacrynic Acid
Izuchenie mekhanizma deĭstviia diuretikov metodom fliuorestsentnogo zonda.