Ricin B chain converts a non-cytotoxic antibody-ricin A chain conjugate into a potent and specific cytotoxic agent.
We report the conversion of a non-cytotoxic antibody-ricin A chain conjugate to one displaying specific cytotoxic effects comparable with that of native ricin, by the addition of ricin B chain as a second stage reagent. The results suggest that this conversion is achieved by the association of the added B chain with the A chain of the conjugate, and not through a primary binding of B chain at the cell surface.
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005354:Fibrosarcoma / Q000276:immunology*; D051381:Rats; D012276:Ricin / Q000633:toxicity*; D013329:Structure-Activity Relationship
D000911:Antibodies, Monoclonal; D012276:Ricin