[Comparative study of laminarinases from marine mollusks using differential UV-spectrophotometry].
A comparative study of tryptophane residues in endo-beta-1.3-glucanases from marine molluscs was carried out by UV-differential spectrophotometry and chemical modification with N-bromosuccinimide methods. The number of tryptophane and tyrosine residues exposed and accessible to the solvent was determined, using a solvent perturbation technique with 20% ethylene glycol. The alteration in the accessibility of these residues in 8 M urea and in the presence of the reaction substrate was shown. The tryptophane residues of endo-beta-1.3-glucanases were found to participate in the enzyme-substrate interaction. The interaction between endo-beta-1.3-glucanases and the substrate, laminarine, as well as between the substrate analogs and inhibitors--cellobiose and glucone-1,5-lactone, was investigated.
D000818:Animals; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007700:Kinetics; D008974:Mollusca / Q000201:enzymology*; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014364:Tryptophan / Q000032:analysis
D014364:Tryptophan; D006026:Glycoside Hydrolases; D004693:Glucan Endo-1,3-beta-D-Glucosidase
Sravnitel'noe izuchenie laminarinaz iz morskikh molliuskov metodom raznostnoĭ UF-spektrofotometrii.