[Determination of the influenza virus concentration in chromatographic vaccinal preparations].
Concentration of influenza virus and its surface antigens in inactivated chromatographic vaccines was evaluated to an accuracy of 10% by determinations of total protein concentrations in them. This approach to the evaluation of virus concentration is possible because in the currently released inactivated chromatographic influenza vaccines at least 90% of the total protein consist of virus particle protein. This is based on the analysis of virus vaccines made by polyacryl amide gel-sodium dodecyl sulphate electrophoresis. It was calculated that 3 X 10(11) virus particles in 1 ml correspond to 100 micrograms/ml virus protein (approximate concentration of protein in vaccine preparations).
D000954:Antigens, Surface / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D009980:Influenza A virus / Q000032:analysis* / Q000276:immunology; D007252:Influenza Vaccines / Q000032:analysis*; D014613:Vaccines, Attenuated / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis; D014771:Virion / Q000032:analysis / Q000276:immunology
D000954:Antigens, Surface; D000956:Antigens, Viral; D007252:Influenza Vaccines; D014613:Vaccines, Attenuated; D014764:Viral Proteins
Opredelenie kontsentratsii virusa grippa v khromatograficheskikh vaktsinal'nykh preparatakh.