Immunohistochemical demonstration of beta-enolase in human skeletal muscle.
We have localized beta-enolase activity in human skeletal muscle fiber using the immunohistochemical method (two-step method). The first immunoreagent was rabbit anti-human beta-enolase serum raised in New Zealand white rabbit, and the second was peroxidase conjugated staphylococcal protein A. The immunohistochemical reaction for beta-enolase was noted higher in type 2 fiber, which demonstrates the low oxidative and high glycolytic enzyme activity, than in type 1 fiber.
D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D007120:Immunochemistry / Q000379:methods; D009132:Muscles / Q000201:enzymology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*
D010751:Phosphopyruvate Hydratase