A discontinuous electrophoretic system for separating peptides on polyacrylamide gels.
An electrophoretic system for separating, with high resolution, peptides 25-250 residues in length is described. The peptides are stacked by discontinuous electrophoresis to form very sharp bands at the origin. They are then separated on a matrix of 20% polyacrylamide, 8 M urea, and 0.1% dodecyl sulfate. Through this combination, high resolution and clean separation, based on polymer length, are achieved.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D010446:Peptide Fragments / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate; D014508:Urea
D010446:Peptide Fragments; D010455:Peptides; D012967:Sodium Dodecyl Sulfate; D014508:Urea