Mutagenicity studies of ambient airborne particles. II. Comparison of extraction methods.
Organic materials were extracted with acetone from airborne particles by shaking, soxhletion and sonication for varying durations. 4-h, 1-h and 1/8-min extractions by shaking, soxhletion and sonication, respectively gave maximum his+ revertants with the Ames Salmonella/microsome assay. In a comparative study of extraction methods, sonication gave the highest and soxhletion the lowest mutagenic response. It appears that sonication with acetone is the best procedure for the extraction of mutagens from airborne particles as shown by Ames assay and Arar assay systems in Salmonella typhimurium.
D000393:Air Pollutants / Q000302:isolation & purification* / Q000633:toxicity; D000818:Animals; D001711:Biotransformation; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000302:isolation & purification*; D051381:Rats
D000393:Air Pollutants; D009153:Mutagens