[Binding of IgG and its aggregates to human platelets can be made saturable by using deoxyglucose and antimycin A].
Binding of bisdiazoniumbenzidine cross-linked (BDB) and monomeric IgG to washed human platelets at 37 degrees C was assessed. Metabolic inhibitors and prostaglandin E1 permitted study of partially reversible and saturable binding of the ligands to platelets: in presence of 0.25 microM antimycin A and 6 mM deoxyglucose, maximal binding of 3H-BDB-IgG (10 min, 37 degrees C) was 0.52 fg per platelet and of 3H-IgG (20 min, 37 degrees C) 0.13 fg per platelet. In presence of 6 mM glucose, however, the amount of IgG bound per platelet was higher and not saturable. These findings suggest that IgG is actively engulfed by the platelets.
D000968:Antimycin A; D001560:Benzidines; D001665:Binding Sites; D001792:Blood Platelets / Q000276:immunology / Q000378:metabolism*; D003837:Deoxy Sugars; D003847:Deoxyglucose; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007700:Kinetics; D046911:Macromolecular Substances; D011485:Protein Binding
D001560:Benzidines; D003837:Deoxy Sugars; D007074:Immunoglobulin G; D046911:Macromolecular Substances; C006770:bis(diazo)benzidine; D000968:Antimycin A; D003847:Deoxyglucose
Die Bindung von IgG und Aggregaten davon an menschliche Thrombozyten kann durch Verwendung von Deoxyglucose und Antimycin A zur Sättigung gebracht werden.