[Molecular mechanisms of interphase death of lymphoid cells. Distribution of nuclease attack sites in chromatin and kinetic complexity of DNA in PDN].
On the basis of a quantitative correlation between single--and double-helix fragments of different length within PDN, a study was made of the pattern of distribution of the sites, attacked by nuclease, in the chromatin. It was shown that under the effect of ionizing radiation products of enzymic digestion of the chromatin. It was shown that under the effect of ionizing radiation products of enzymic digestion of the chromatin accumulated in thymocytes due to internucleosome degradation were excised from randomly localized genome sites. The analysis of the reassociation curves did not reveal distinctions in the kinetic complexity of the fractions of PDN and total DNA.
D000818:Animals; D002470:Cell Survival / Q000528:radiation effects*; D002843:Chromatin / Q000528:radiation effects*; D004247:DNA / Q000032:analysis*; D003852:Deoxyribonucleoproteins / Q000528:radiation effects; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D007399:Interphase; D008297:Male; D011089:Polydeoxyribonucleotides / Q000032:analysis*; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid / Q000528:radiation effects; D013950:Thymus Gland / Q000166:cytology* / Q000528:radiation effects
D002843:Chromatin; D003852:Deoxyribonucleoproteins; D011089:Polydeoxyribonucleotides; D004247:DNA; D004706:Endodeoxyribonucleases; C029367:calcium magnesium dependent endodeoxyribonuclease
Molekuliarnye mekhanizmy interfaznoĭ gibeli limfoidnykh kletok. Kharakter raspredeleniia mest nukleaznoĭ ataki v khromatine i kineticheskaia slozhnost' DNK v sostave PDN.