In vivo flux between phosphocreatine and adenosine triphosphate determined by two-dimensional phosphorous NMR.
The in vivo unidirectional flux between creatine phosphate and ATP was determined in the leg and head of the anesthetized rat using a two-dimensional NMR technique. The unidirectional flux of creatine phosphate to ATP was 13 mumol/s/g-weight in the leg and 2 mumol/s/g-weight in the head. The unidirectional flux between ATP and inorganic phosphate was too slow to be detected and was estimated to be less than 0.1 mumol/s/g-weight.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D009132:Muscles / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D014018:Tissue Distribution
D010725:Phosphocreatine; D000255:Adenosine Triphosphate