Structural determinants of Phaseolus vulgaris erythroagglutinating lectin for oligosaccharides.
The behaviors of oligosaccharides obtained by hydrazinolysis of asparagine-linked sugar chains in an erythroagglutinating phytohemagglutinin lectin-agarose column were examined to define the structural determinants required for the interaction of asparagine-linked sugars with erythroagglutinating phytohemagglutinin. The minimal structural unit required for erythroagglutinating phytohemagglutinin binding was (formula; see text) in which R1 and R2 represent either hydrogen or sugars and R3 represents either GlcNAc leads to Asn or (Fuc alpha 1 leads to 6)GlcNAcOT.
D002236:Carbohydrate Conformation; D002846:Chromatography, Affinity; D009844:Oligosaccharides / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000378:metabolism*; D013329:Structure-Activity Relationship
D009844:Oligosaccharides; D010835:Phytohemagglutinins