Microcalorimetry studies of energy changes during the growth of Klebsiella aerogenes in simple salts/carbon-limited media: growth in the presence of glucose and alpha-methyl-D-glucoside.
The power-time profile and the derived thermal parameters obtained during the growth of glucose-adapted cells of K. aerogenes in glucose-limited medium containing added alpha-methyl-D-glucoside (alpha-MG) are markedly different from those obtained during growth in glucose alone. The power-time profile changes in a regular manner with increasing concentrations of alpha-MG; the rate constants for exponential growth and heat output decrease linearly. Yield and enthalpy data indicate that alpha-MG is not metabolized, and this is not supported by the data for carbon dioxide production. The increase in the total cell volume which occurs as a result of growth in the presence of alpha-MG indicates that this carbon source is possibly stored. The power-time profile and thermal parameters for alpha-MG trained cells, grown with alpha-MG limitation and in mixed carbon-limited media are reported. In mixed media catabolic repression occurs.
D002151:Calorimetry / Q000379:methods; D003470:Culture Media; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D007709:Klebsiella / Q000254:growth & development / Q000378:metabolism*; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*
D003470:Culture Media; D008757:Methylglucosides; D008759:Methylglycosides; D005947:Glucose