Disulfide linking of albumin to the hinge region of immunoglobulin G in normal human serum.
A protein-protein complex from human serum was isolated and characterized as a two-component system, containing albumin and IgG in a 1:1 mole ratio. The observations reported here suggest that the albumin-IgG complex may have formed through intermolecular disulfide bonds between albumin and IgG. Immunoelectrophoretic analysis of papain-digested fragments of albumin-IgG complex revealed that the combining sites for albumin may be located in the hinge region between the Fab and the Fc fragments of IgG.
D000328:Adult; D000596:Amino Acids / Q000032:analysis; D001665:Binding Sites; D004220:Disulfides / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000378:metabolism*; D008297:Male; D012709:Serum Albumin / Q000378:metabolism*
D000596:Amino Acids; D004220:Disulfides; D007074:Immunoglobulin G; D012709:Serum Albumin