title: Discrepancies in measurement of aspartate aminotransferase by continuous-flow analysis.
abstract: I compared results for aspartate aminotransferase (EC 2.6.1.1) obtained with a reaction-rate analyzer (LKB 2086 Mark Two), based on IFCC methodology, and a continuous-flow analyzer (the Technicon SMA 2) for 115 patients' sera and seven commercial quality-control sera. The data from the SMA 2 showed a clear positive bias in those sera with activities exceeding 40 U/L (the upper limit of the reference range). Independent data to support the bias of the SMA 2 and other continuous-flow analyzer systems are presented. Application of a correction factor to the SMA 2 data above the upper limit of the range significantly decreased this bias. Failure to apply such a factor to data obtained from continuous-flow analyzers could lead to serious clinical misinterpretation.
mesh_terms: D001219:Aspartate Aminotransferases / Q000097:blood*; D001322:Autoanalysis / Q000295:instrumentation; D003951:Diagnostic Errors; D005069:Evaluation Studies as Topic; D006801:Humans
chemical_list: D001219:Aspartate Aminotransferases