An intermediate in the biosynthesis of vasopressin and neurophysin in the guinea pig posterior pituitary.
Biosynthesis of oxytocin (OT), vasopressin (AVP) and neurophysins (NP) has been studied in the guinea pig using incorporation of radiolabelled cysteine and fucose, and analysis by high pressure liquid chromatography and radioimmunoassay. Using these methods it was possible to identify and quantitate OT, AVP and their respective NPs as well as the carboxy-terminal glycopeptide derived from the AVP precursor. In addition, we have also identified an intermediate component comprising the AVP-related NP with its carboxy-terminal glycopeptide still attached. The presence of this intermediate suggests that the removal of AVP is the first step in the proteolytic cleavage of the AVP precursor.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs / Q000378:metabolism*; D008297:Male; D009481:Neurophysins / Q000096:biosynthesis*; D010121:Oxytocin / Q000378:metabolism; D010904:Pituitary Gland, Posterior / Q000378:metabolism*; D011863:Radioimmunoassay; D014667:Vasopressins / Q000096:biosynthesis*
D009481:Neurophysins; D014667:Vasopressins; D010121:Oxytocin