[Absorption routes of stearic acid and leucine in the small intestine of the rat after gamma irradiation].
The aim of the investigation was to study transport of 3H-stearic acid in ultrastructural compartments of epitheliocytes during absorbtion, resynthesis and translocation of lipids having the form of chylomicrons after a local large fractionated gamma-irradiation of the rat small intestine with a total dose of 35 Gr. In the anesthesized animals the ligated fragments of the jejunum, which kept their blood supply and innervation, were separated, fatty emulsion with 3H-stearic acid (a label) was administered into their lumen. Intensity of the label incorporation was quantitatively estimated in the electron microscopic autographs every 2, 5, 10 and 20 min after the isotope administration. Time distribution of the label concentration after irradiation characterized the sequence of the transport stages of 3H-stearic acid across microvilli and the terminal reticulum (MV + TR) triglycerides resynthesis in the agranular and granular endoplasmic reticulum (AER, GER), chylomicrons formation in Golgi complex (GC), as well as their exudation into the lacunar intercellular space (LIS), basal membrane and capillaries. Transport deceleration of the label at the level MV + TR and decreasing synthesis of triglycerides in GER was accompanied with a delay in the chylomicrons formation in the GC and their excretion into the LIS. Conglomerates of the aggregated chylomicrons were formed in the LIS. The radioautographic data on 3H-leucine incorporation characterized a decreased activity of the protein-synthesizing system of epitheliocytes.
D000818:Animals; D002914:Chylomicrons / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D007408:Intestinal Absorption / Q000528:radiation effects*; D007413:Intestinal Mucosa / Q000648:ultrastructure; D007930:Leucine / Q000378:metabolism*; D008297:Male; D008871:Microvilli / Q000378:metabolism; D051381:Rats; D013229:Stearic Acids / Q000378:metabolism*
D002914:Chylomicrons; D013229:Stearic Acids; C031183:stearic acid; D007930:Leucine
Puti vsasyvaniia stearinovoĭ kisloty i leĭtsina v tonkoĭ kishke krysy posle gamma-oblucheniia.