Uptake and binding of cytoplasmic DNA by wheat embryo cell nuclei.
Cytoplasmic non-mitochondrial DNA was selectively labelled and isolated In a linear single-stranded form from early wheat embryos. The isolated preparation was readily taken up by wheat embryo cell nuclei and firmly bound to large, chromosome-like structures. Similarly prepared nuclear DNA fragments, although taken up, remained unbound and underwent a rapid degradation within the cell nuclei. The selective binding of the cytoplasmic DNA indicates that it might be integrated into the nuclear genome.
D002467:Cell Nucleus / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D014908:Triticum / Q000235:genetics*
D004247:DNA