Use of a cell harvester for the collection of tissue fragments in receptor binding assays.
The use of a commercially available cell harvester in radioreceptor binding assays is described. Scatchard analyses of data acquired using the cell harvester is presented for [3H]clonidine, [3H]prazosin, [3H]quinuclidinyl benzilate (QNB), and [3H]flunitrazepam. Dissociation constants (Kd) and maximum number of binding sites (Bmax) obtained for each ligand are in good agreement with those published by others and there is excellent intra- and interassay agreement. Apparent inhibition constants for various common drugs are also included. Analyses of precision, efficiency, and cost support the use of this cell harvester in binding assays.
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D002469:Cell Separation / Q000295:instrumentation*; D003000:Clonidine / Q000378:metabolism; D005374:Filtration / Q000295:instrumentation*; D005445:Flunitrazepam / Q000378:metabolism; D007700:Kinetics; D011224:Prazosin / Q000378:metabolism; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains
D005445:Flunitrazepam; D011813:Quinuclidinyl Benzilate; D003000:Clonidine; D011224:Prazosin