Anchorage dependence and Ca2+ requirement are independently modulated by hydrocortisone hormone in rat C6 glioma cells.
We had previously shown that hydrocortisone (Hy), a glucocorticoid hormone, regulates the expression of the transformed phenotype of rat C6 glioma cells. The hormone reversibly renders C6 cells dependent on anchorage and high Ca2+ concentration for growth. We had also isolated Hy-resistant C6 variants in agarose suspension cultures. Here we report that Hy-resistant variants selected in high (1.8mM) Ca2+ medium become growth-arrested in low (30 microM) Ca2+ medium upon hormone treatment. We conclude that Hy-induced anchorage dependence and Hy-induced high Ca2+ requirement for growth of C6 glioma cells, are two independent phenotypes.
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D005910:Glioma / Q000503:physiopathology*; D006854:Hydrocortisone / Q000494:pharmacology*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D051381:Rats
D003470:Culture Media; D008274:Magnesium; D002118:Calcium; D006854:Hydrocortisone