Induction of liver microsomal 2-hydroxylation of estradiol 17-sulfate by phenobarbital in male rat.
Estradiol 17-sulfate 2-hydroxylase activity was measured in liver microsomes of 7- to 8-weeks-old Wistar male rats. Phenobarbital (80 mg/kg, daily for 4 d) injected intraperitoneally caused 30% increase of the enzyme activity and 160% increase of the microsomal cytochrome P-450 content. Treatment of rats with 3-methylcholanthrene (40 mg/kg, daily for 4 d) injected intraperitoneally caused no induction effect on 2-hydroxylase activity, but P-450 content was increased by 113%. Both the P-450 content and the enzyme activity were reduced to about half of the control, when the animals were injected subcutaneously with cobaltous chloride (30 mg/kg, daily for 2 d).
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003035:Cobalt / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism*; D006900:Hydroxylation; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D008862:Microsomes, Liver / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D003035:Cobalt; D004958:Estradiol; D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System; C018021:cobaltous chloride; D010634:Phenobarbital