6 beta-hydroxycortisol in random urine samples as an indicator of enzyme induction.
6 beta-Hydroxycortisol (6 beta-OHF) is a major cortisol metabolite formed primarily in the hepatic reticulum by mixed-function oxygenases. Changes in 6 beta-OHF may be used to detect enzyme induction by drugs or disease. Excretion of 6 beta-OHF must be corrected for change in urinary free cortisol (FF) excretion to allow for changes in cortisol production. We report the absence of circadian changes in 6 beta-OHF/FF ratios. Thus random measurements of 6 beta-OHF/FF ratios may be used to detect enzyme induction. This approach greatly simplifies noninvasive screening for enzyme induction.
D002648:Child; D002675:Child, Preschool; D002940:Circadian Rhythm; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000031:analogs & derivatives* / Q000652:urine; D008297:Male; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*; D010634:Phenobarbital / Q000494:pharmacology
C001380:6 beta-hydroxycortisol; D006899:Mixed Function Oxygenases; D006854:Hydrocortisone; D010634:Phenobarbital