Coexistence of cardiac-type and fast skeletal-type myosin light chains in embryonic chicken cardiac muscle.
Myosin from embryonic chicken ventricle contained a light chain component which comigrated with fast skeletal myosin light chain 1 (Lf1) on two dimensional electrophoresis in addition to cardiac type light chains (Lc1 and Lc2). Immunoblot analysis showed that this minor light chain band reacted with anti-Lf1 antibody. Antigens binding with anti-Lc1 and anti-Lf1 antibodies were located on myofibrils in embryonic cardiac muscle cells in vivo and in vitro. From these observations, we conclude that a small amount of Lf1 exists in embryonic chicken cardiac muscle.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D009132:Muscles / Q000196:embryology / Q000378:metabolism*; D009218:Myosins / Q000302:isolation & purification* / Q000378:metabolism
D009218:Myosins