Comparisons using 35S- and 32P-labeled DNA for hybridization on nitrocellulose filters.
DNA was labeled by nick translation with 35S and used as a probe in Southern- or colony-blot DNA hybridization. Comparison with DNA labeled with 32P showed that not only was 35S-labeled DNA suitable as a probe, but in many cases had advantages. The longer half-life of 35S allows for less stringent timing of experiments and eliminates the waste of unused old label. Resolution on autoradiographs was found to improve when using 35S-labeled DNA.
D055598:Chemical Phenomena; D002621:Chemistry; D003101:Collodion; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005374:Filtration; D007553:Isotope Labeling; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D013462:Sulfur Radioisotopes
D004269:DNA, Bacterial; D010761:Phosphorus Radioisotopes; D013462:Sulfur Radioisotopes; D003101:Collodion