Failure to find amyloidosis in dogs treated with long-term intravenous insulin delivered by a totally implantable pump.
We examined tissues of seven non-diabetic mongrel dogs and four diabetic beagle dogs treated with constant insulin infusion via totally implantable pumps for from 210 to 880 days. Kidney and skeletal muscle tissue from all dogs were stained with Congo Red and thioflavin-T and appropriately examined. Kidney tissues from the beagle dogs were examined by electron microscopy. No amyloid deposits were found in any of these tissues. Thus, we cannot confirm an earlier report of amyloid occurring in dogs given long-term intravenous insulin. It is concluded that amyloidosis is not a necessary complication of long-term intravenous insulin infusion in dogs.
D000686:Amyloidosis / Q000209:etiology*; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D004285:Dogs; D005260:Female; D007332:Insulin Infusion Systems / Q000009:adverse effects*; D007668:Kidney / Q000648:ultrastructure; D008297:Male; D009132:Muscles / Q000648:ultrastructure