Vibrio cholerae expresses iron-regulated outer membrane proteins in vivo.
A comparison was made, using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, of the outer membrane proteins of four strains of Vibrio cholerae grown in vivo in infant rabbits and in vitro in low-iron and iron-supplemented defined media. In vivo-grown V. cholerae expressed novel outer membrane-associated proteins which, in part, were similar to those observed on V. cholerae grown in vitro under conditions of iron deprivation.
D000818:Animals; D001345:Autoradiography; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D002771:Cholera / Q000378:metabolism / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D007501:Iron / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D011233:Precipitin Tests; D011817:Rabbits; D014734:Vibrio cholerae / Q000378:metabolism*
D001426:Bacterial Proteins; D008565:Membrane Proteins; D007501:Iron