Colony growth in soft agar of human melanoma cells from biopsy material, cell lines and xenografts.
Colony growth in soft agar of human melanoma cells from biopsy material, cell lines and xenografts was evaluated. Colony forming potential is constantly very low in all studied types of tumor tissue, however slight increase in clonogenic potential was seen during subsequent xenograft passages, but within the range of less than 1%. Results of presented studies suggest that the number of colonies does necessarily express the degree of colony forming potential and the culture preparation and conditions as well. It appears that there is no reliable correlation between the number of colonies in soft agar and the clinical course of melanoma patients under study.
D000362:Agar; D000818:Animals; D001706:Biopsy; D002478:Cells, Cultured; D006801:Humans; D066298:In Vitro Techniques; D008545:Melanoma / Q000473:pathology*; D051379:Mice; D012878:Skin Neoplasms / Q000473:pathology*; D014183:Transplantation, Heterologous; D014410:Tumor Stem Cell Assay
D000362:Agar