title: Cholera toxin genes: nucleotide sequence, deletion analysis and vaccine development.
abstract: Nucleotide sequence and deletion analysis have been used to identify the regulatory and coding sequences comprising the cholera toxin operon (ctx). Incorporation of defined in vitro-generated ctx deletion mutations into Vibrio cholerae by in vivo genetic recombination produced strains which have practical value in cholera vaccine development.
mesh_terms: D001483:Base Sequence; D002772:Cholera Toxin / Q000235:genetics*; D022121:Cholera Vaccines; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D009876:Operon; D011995:Recombination, Genetic; D014734:Vibrio cholerae / Q000235:genetics
chemical_list: D022121:Cholera Vaccines; D002772:Cholera Toxin