title: Evaluation of an affinity chromatographic procedure for the determination of glycosylated hemoglobin (HbA1).
abstract: An affinity chromatographic method for the determination of glycosylated hemoglobin (HbA1) was evaluated. The procedure was shown to be precise, the within- and between-assay coefficients of variation being less than 5%. It was also shown to correlate well with electrophoresis (r = 0.968) and ion-exchange chromatography (r = 0.916). An inverse relationship was shown to exist between increasing temperature and HbA1 levels measured by affinity chromatography. A statistically significant difference was found for samples run at 20 degrees C and 25 degrees C respectively, suggesting that the method should be run in a temperature-controlled environment. The affinity procedure was also shown not to be affected by the type of anticoagulant, the concentration of hemoglobin in the hemolysate, and acetylation.
mesh_terms: D000107:Acetylation; D000925:Anticoagulants; D002846:Chromatography, Affinity; D003920:Diabetes Mellitus / Q000097:blood; D006442:Glycated Hemoglobin / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D013696:Temperature
chemical_list: D000925:Anticoagulants; D006442:Glycated Hemoglobin; D006023:Glycoproteins