title: Studies on avian erythrocyte metabolism. XIII. Changing organic phosphate composition in age-dependent density populations of chicken erythrocytes.
abstract: These studies were performed to determine whether the erythrocytes (RBC's) of chickens are able to alter the concentrations of organic phosphates, particularly inositol pentakis (dihydrogen phosphate) (inositol-P5), in response to anemia. Acute anemia with 33.5% reticulocytes (retics) in the peripheral blood of 4-month-old White Leghorn cockerels was produced after two successive days of controlled bleeding. Five populations of RBC's were prepared from blood of these anemic birds based upon age-dependent density by angle rotor centrifugation. The relative increase in age of Fraction 1 (younger cells) through Fraction 5 (oldest cells) was verified by increased hemoglobin concentration and decreased retic count and cell volume. The acid aqueous-soluble phosphates from each RBC fraction were extracted, fractionated by ion exchange column chromatography, and the concentration of inorganic phosphate (Pi), adenosine triphosphate (ATP), and inositol-P5 quantitated by wet-ash phosphate analysis. Determination of Pi, ATP, and inositol-P5 in Fraction 1 (younger cells, 7.4% retics) from the initial (Day 1) blood withdrawn gave 4.3, 5.7, and 18.6 mumoles Pi/ml RBC, respectively. On Day 4 of controlled-bleeding, Pi, ATP, and inositol-P5 concentrations in Fraction 1 (95.1% retics) were 12.7, 10.6, and 12.3 mumoles Pi/ml RBC, respectively. On Day 11, after 1 week recovery from controlled-bleeding, the values for Pi, ATP, and inositol-P5 in Fraction 1 (37.7% retics) were 5.0, 5.4, and 19.0 mumoles Pi/ml RBC, respectively, which were similar to the values before bleeding.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000097:blood; D000818:Animals; D002645:Chickens / Q000097:blood*; D004163:Diphosphoglyceric Acids / Q000097:blood; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000378:metabolism*; D006400:Hematocrit / Q000662:veterinary; D006454:Hemoglobins / Q000378:metabolism; D007295:Inositol Phosphates / Q000097:blood; D008297:Male; D010710:Phosphates / Q000097:blood*; D012156:Reticulocytes / Q000378:metabolism*
chemical_list: D004163:Diphosphoglyceric Acids; D006454:Hemoglobins; D007295:Inositol Phosphates; D010710:Phosphates; D019794:2,3-Diphosphoglycerate; C013571:inositol pentaphosphate; D000255:Adenosine Triphosphate