Dot-blotting--a novel screening assay for antibodies in hybridoma cultures.
A novel solid phase radioimmunoassay using nitrocellulose filters, is described. The method is of particular value in screening hybridoma cultures for monoclonal antibodies against chromosomal protein. Also it permits detection of antibodies directed against epitopes stable to SDS denaturation.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000936:Antigen-Antibody Complex; D000941:Antigens / Q000032:analysis; D002875:Chromosomes / Q000032:analysis*; D003101:Collodion; D006224:Cricetinae; D003412:Cricetulus; D006825:Hybridomas / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009698:Nucleoproteins / Q000032:analysis*; D010209:Paper; D011863:Radioimmunoassay / Q000379:methods
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000941:Antigens; D009698:Nucleoproteins; D003101:Collodion