Hormone-stimulated lipolysis in cardiac myocytes.
Type L hormone-sensitive lipase (HSL) activity was increased approx. 35% above control in cardiac myocytes incubated for 15 min with 5 nM-adrenaline. Concomitantly. adrenaline-stimulated myocytes had a lower triacylglycerol content, released more non-esterified fatty acid and had a higher intracellular concentration of cyclic AMP than did myocytes incubated without hormone. The lipase activity measured in adrenaline-stimulated and non-stimulated myocytes was stable in acetone/diethyl ether, stimulated by serum and inhibited by NaCl. These properties are consistent with the type L designation of this HSL. The finding that type L HSL is stimulated by adrenaline indicates that the enzyme that is being activated is found in the cell and not associated with an extracellular compartment of the myocardium.
D000818:Animals; D002451:Cell Compartmentation; D004789:Enzyme Activation / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D008066:Lipolysis / Q000187:drug effects*; D008071:Lipoprotein Lipase / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D008071:Lipoprotein Lipase; D004837:Epinephrine