High-performance liquid chromatographic method for the determination of plasma allantoin.
A new method has been devised for the determination of nanomole levels of allantoin in human plasma. Allantoin was converted into xanthylallantoin, which was chromatographed on a reversed-phase silica gel using a mixture of acetonitrile-water (27:73) as mobile phase. The eluted compound was measured using an ultraviolet detector. The detection limit of the assay for plasma was about 100 ng/ml. This method was applied successfully to the determination of allantoin in human plasma after oral administration of 100 mg of aldioxa.
D000328:Adult; D000481:Allantoin / Q000031:analogs & derivatives / Q000032:analysis / Q000097:blood* / Q000138:chemical synthesis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D006801:Humans; D008875:Middle Aged; D013997:Time Factors
D000481:Allantoin; C039717:xanthylallantoin