[Kinetic method for determination of isoenzymes of alkaline phosphatase in human blood serum].
Resistance of liver, bone, intestinal and placental human alkaline phosphatase isozymes to the effect of L-phenylalanine and urea was studied. The combined treatment with urea and L-phenylalanine increased an accuracy of estimation due to inhibition of bone and intestinal isozymes. A special equation enabled to simplify the calculations. As shown by laboratory and clinical tests, the method developed is suitable for quantitative estimation of alkaline phosphatase isoenzymes.
D000469:Alkaline Phosphatase / Q000097:blood*; D001842:Bone and Bones / Q000201:enzymology; D004796:Clinical Enzyme Tests; D006801:Humans; D007422:Intestines / Q000201:enzymology; D007527:Isoenzymes / Q000097:blood*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D010649:Phenylalanine / Q000494:pharmacology; D010920:Placenta / Q000201:enzymology; D014508:Urea / Q000494:pharmacology
D007527:Isoenzymes; D010649:Phenylalanine; D014508:Urea; D000469:Alkaline Phosphatase
Kineticheskiĭ metod opredeleniia izofermentov shchelochnoĭ fosfatazy syvorotki krovi cheloveka.