Genetic effects of radiocarbon in reproductive cells of male mice.
The genetic effect of incorporated radiocarbon was studied after single, long-term (33 days) and chronic (6 and 12 months) treatment of male mice (CBA X C57B1) F1 with [14C]glucose. The genetic effect in male germ cells was estimated by 3 tests: DLM frequency in post- and pre-meiotic cells, RT frequency in stem spermatogonia and frequency of abnormal sperm heads. Absorbed doses in the gonads were: 0.22, 0.50 and 1.01 Gy, after a single exposure; 0.74 and 1.47 Gy, after long-term exposures; and 0.006 and 0.031 Gy, after chronic exposure for 6 months; and 0.013 and 0.066 Gy, for 12 months. The results suggest that DLM frequency in post-meiotic cells increased linearly with increasing the dose of 14C single and long-term exposures at a dose of 1.47 Gy only. A chronic treatment with [14C]glucose induced no increase in DLM frequency. RT frequency in stem spermatogonia was statistically significantly higher than the control level after the single and long-term exposure to 14C. A comparison of the results with the results of external single and chronic gamma-irradiation allows the conclusion that the relative genetic efficiency of radiocarbon as compared with that of gamma-rays is about 1.
D000818:Animals; D002250:Carbon Radioisotopes / Q000009:adverse effects*; D004307:Dose-Response Relationship, Radiation; D004334:Drug Administration Schedule; D005799:Genes, Dominant / Q000528:radiation effects*; D005804:Genes, Lethal / Q000528:radiation effects*; D005947:Glucose / Q000008:administration & dosage; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009154:Mutation; D012098:Reproduction / Q000528:radiation effects*; D013093:Spermatogonia / Q000528:radiation effects*; D013094:Spermatozoa / Q000528:radiation effects*; D014178:Translocation, Genetic
D002250:Carbon Radioisotopes; D005947:Glucose