Liquid chromatographic determination and mass spectrometric confirmation of chloramphenicol residues in animal tissues.
A rapid and sensitive liquid chromatographic (LC) method for determination of trace amounts of chloramphenicol (CP) in animal tissues has been developed. Chloramphenicol identity is confirmed by gas chromatography-mass spectrometry (GC-MS). CP can be routinely quantitated by LC at 10 ppb with 75% recovery, and GC-MS selected ion monitoring permits complete identification at concentrations as low as 5 ppb. Residues were determined in chicken tissues after intramuscular injection of CP. Depletion was rapid with no residues detected in muscle after 8 h, while 15-30 ppb CP was still determined and identified in the liver.
D000818:Animals; D002645:Chickens; D002701:Chloramphenicol / Q000032:analysis* / Q000627:therapeutic use; D002853:Chromatography, Liquid; D005506:Food Contamination / Q000032:analysis*; D013058:Mass Spectrometry; D011201:Poultry Diseases / Q000188:drug therapy
D002701:Chloramphenicol