Purification of peanut agglutinin by affinity chromatography on asialoglycophorin from human erythrocytes.
A simple and efficient method of purification of peanut agglutinin is described. This method involves affinity chromatography on human asialoglycophorin linked to Affi-Gel. One milliliter of the packed adsorbent contained 1.7 mg of covalently linked asialoglycophorin and bound about 6 mg of the peanut agglutinin. From 250 g of row peanuts 510 mg of the electrophoretically homogeneous lectin with high agglutinating activity was obtained.
D001212:Asialoglycoproteins; D002846:Chromatography, Affinity; D004912:Erythrocytes; D006023:Glycoproteins; D006801:Humans; D037102:Lectins / Q000302:isolation & purification*; D019887:Peanut Agglutinin
D001212:Asialoglycoproteins; D006023:Glycoproteins; D037102:Lectins; D019887:Peanut Agglutinin; C026503:asialoglycophorin