Protein and antibody in lavage fluid of guinea pigs with Legionella pneumophila pneumonia.
Guinea pigs were infected in an inhalation facility that limited an aerosol of L. pneumophila to the snout, as previously reported in detail (Davis et al., 1982). Individual animals were sacrificed for study either immediately after exposure, at 16 hours, at days one through seven, or at 11 days. Bronchoalveolar lavage was carried out to obtain fluid to study the following: total protein, albumin and immunoglobulin G (IgG) concentrations, and the titer of antibody to L. pneumophila. Antibody also was measured in serum obtained at the time of sacrifice. Concentrations of total protein, albumin, and IgG in lavage fluids peaked 2 days after exposure and correlated with the appearance of maximal numbers of polymorphonuclear cells in the lungs. Presumably, this increased protein resulted from exudation of serum across the alveolar-capillary membrane, which loses its integrity secondary to pneumonia. However, the ratio of IgG/albumin was elevated in animals studied 11 days after exposure even though the concentration of albumin was normal by this time. One possible explanation for this observation is that IgG was being produced in the lung. Antibody in lavage fluid was detected 7, and 11 days post-exposure, and might be important in the recovery of guinea pigs from this infection.
D000418:Albumins / Q000032:analysis; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D006168:Guinea Pigs; D007074:Immunoglobulin G / Q000032:analysis; D007877:Legionnaires' Disease / Q000378:metabolism*; D008168:Lung / Q000032:analysis*; D011014:Pneumonia / Q000378:metabolism*; D011506:Proteins / Q000032:analysis*; D007507:Therapeutic Irrigation
D000418:Albumins; D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D011506:Proteins