Removal of fatty acids from serum albumin by Lipidex 1000 chromatography.
Fatty acids can be effectively removed from serum albumin preparations by a single passage through a column of Lipidex 1000 at 37 degrees C. The procedure is easier and milder and shows a better (nearly quantitative) recovery of protein than charcoal treatment. The ability for fatty acid binding by the protein is not affected by either procedure.
D000818:Animals; D002417:Cattle; D002845:Chromatography / Q000379:methods; D005227:Fatty Acids / Q000097:blood / Q000302:isolation & purification*; D006801:Humans; D012709:Serum Albumin; D012710:Serum Albumin, Bovine
D005227:Fatty Acids; D012709:Serum Albumin; D012710:Serum Albumin, Bovine