Studies on a new nucleoside antibiotic, dapiramicin. I. Producing organism, assay method and fermentation.
A new antibiotic, dapiramicin, has been isolated from the fermentation broth of Micromonospora sp. SF-1917. Since the dapiramicin exhibited essentially no in vitro activity by usual bioassays, two assay methods for dapiramicin were developed, HPLC and a new bioassay by growth inhibition of fungal mycelia on a slide glass. Fermentation of dapiramicin is also described.
D000935:Antifungal Agents / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D008847:Micromonospora / Q000145:classification / Q000254:growth & development* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D012232:Rhizoctonia / Q000187:drug effects; D012263:Ribonucleosides / Q000302:isolation & purification / Q000633:toxicity
D000935:Antifungal Agents; D012263:Ribonucleosides; C039868:dapiramicin