[Electron microscopic-cytochemical method of determining cell phagolysosomal structures].
A new electron-cytochemical method for detection of phagolysosomal structures in intact cells is proposed, which is more susceptible and simple in comparison with methods described elsewhere. The new method involves the addition of sodium beta-glycerophosphate and Pb(NO3)2 to the medium where phagocytes are incubated with phagocytic material. After the fusion of a phagosome containing the phagocytic material, sodium beta-glycerophosphate (the substrate for a marker lysosomal enzyme--acid phosphatase) and Pb2+ with primary lysosomes, hydrolysis of this substrate occurs with the eventual appearance of a deposit of Pb3(PO4)2.
D000818:Animals; D002478:Cells, Cultured; D006651:Histocytochemistry; D008247:Lysosomes / Q000648:ultrastructure*; D008264:Macrophages / Q000648:ultrastructure; D051379:Mice; D008808:Mice, Inbred CBA; D008854:Microscopy, Electron / Q000379:methods*; D009940:Organoids / Q000648:ultrastructure*; D010586:Phagocytes / Q000648:ultrastructure; D010587:Phagocytosis; D014617:Vacuoles / Q000648:ultrastructure*
Elektronno-tsitokhimicheskiĭ metod opredeleniia fagolizosomal'nykh struktur kletki.