In vitro toxicity and transformation potency of nickel compounds.
An in vitro bioassay utilizing BHK-21 cells in culture is used to determine the relative transformation potential of a number of nickel compounds including, as relatively insoluble particulates a known carcinogen (Ni3S2) and several oxides either of commercial interest or found in the working environment in the metal industry (e.g., NiO), and a soluble salt [Ni(CH3COO)2]. Although a wide range of transformation potency is found as a function of the dose of Ni per area of culture, all substances produce the same number of transformed colonies at the same degree of toxicity (e.g., 50% survival). If toxicity is a direct measure of intracellular concentration, then apparently nickel per se is the ultimate transforming agent independent of source or uptake mechanism.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D006224:Cricetinae; D007668:Kidney; D009532:Nickel / Q000633:toxicity*; D009942:Organometallic Compounds / Q000633:toxicity*; D014896:Welding
D009942:Organometallic Compounds; D009532:Nickel