A simple method for reliable separation of cerebellar Purkinje cell complex and simple spikes.
During the analysis of cerebellar Purkinje cell firing the use of two level discriminators for the separation of complex spike (CS) and simple spike (SS) can produce "wrong SS-events", since the amplitude of the CS wavelets may exceed the discrimination level for the SS. This is also the case, when the initial spike of the CS is negatively deflected. A logic circuit was developed, which ensures reliable separation of the two types of spike by a mutual control of the two channels. The CS wavelet events are obtained via an additional channel.
D000818:Animals; D002415:Cats; D004594:Electrophysiology / Q000379:methods*; D011689:Purkinje Cells / Q000502:physiology*