Serum concentration of bile acids in guinea pigs as an indicator of liver damage caused by aflatoxins.
Serum concentrations of glycocholic acid (GA) and glycodeoxycholic acid (GDA) and serum activity of aspartate aminotransferase (AST) were determined after guinea pigs had been fed 0, 0.005, 0.010, 0.015, 0.020, or 0.030 mg of aflatoxin B1 equivalents daily for 21 days. Mean serum concentrations for the 20 control guinea pigs were GA, 3.70 mumole/L; GDA, 0.12 mumole/L; and AST, 59.7 IU. Concentrations of GA and GDA in treated guinea pigs were significantly higher than those in controls (P less than 0.05) at doses of aflatoxin greater than or equal to 0.010 mg/day. The highest serum concentrations of bile acids were in guinea pigs given the higher doses of aflatoxin. Activities of AST in treated guinea pigs were significantly higher than those in controls for only 2 dosage levels (0.010 and 0.030 mg/day). Bile acids in the serum of guinea pigs was a more sensitive indicator of liver damage caused by aflatoxin than was AST.
D000348:Aflatoxins / Q000633:toxicity*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D003840:Deoxycholic Acid / Q000031:analogs & derivatives*; D006000:Glycocholic Acid / Q000097:blood*; D006002:Glycodeoxycholic Acid / Q000097:blood*; D006168:Guinea Pigs; D008099:Liver / Q000187:drug effects* / Q000473:pathology
D000348:Aflatoxins; D003840:Deoxycholic Acid; D006002:Glycodeoxycholic Acid; D001219:Aspartate Aminotransferases; D006000:Glycocholic Acid