Characterisation of an intermediate in neurophysin biosynthesis in the guinea pig.
In addition to oxytocin (OT), vasopressin (AVP) and their respective neurophysins (NPs), another [35S]cysteine incorporating component is present in the guinea pig neurohypophysis. Gel filtration and Con A affinity chromatography revealed that this component was larger than NP and was glycosylated. NP-immunoreactivity was assessed using antisera which distinguish the OT- and AVP-related NPs. Whilst the anti-NP antiserum detected only one component (guinea pig NP), the anti-NP antiserum detected both NP and the glycosylated 35S-labelled component. These results suggest that a significant amount of NP in guinea pig neural lobes bears a glycopeptide extension and represents a partially processed form of the AVP precursor in this species.
D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006020:Glycopeptides / Q000302:isolation & purification; D006168:Guinea Pigs; D007118:Immunoassay; D009481:Neurophysins / Q000096:biosynthesis*; D010904:Pituitary Gland, Posterior / Q000378:metabolism*; D011498:Protein Precursors / Q000302:isolation & purification
D006020:Glycopeptides; D009481:Neurophysins; D011498:Protein Precursors