Grafting of triggering site onto lymphocytes; distribution of grafted dinitrophenyl groups on cell surface glycoproteins and glycolipids.
Quantitative investigation of membrane-bound sialoglycoconjugates on lymphocyte surface was performed by chemical modification of the sialic acid residues with radioactive N4-dinitrophenyl-L-2,4-diaminobutyric acid hydrazide (DNP-DABH). This labeled both glycoproteins and glycolipids with concomitant preservation of the mitogenic activity by multivalent hapten binding protein (anti-DNP antibody). Under conditions where maximum stimulation of thymocytes occurred radioactive DNP-DABH labeled 1.1 X 10(7) glycolipids molecules/cell but, only 3 X 10(6) glycoproteins molecules/cell. When B lymphocytes, which do not undergo DNP-mediated stimulation were used, glycolipids labeling could not be detected. Major differences between stimulation committed and non-committed DNP-modified lymphocytes was the amount of ligand attached to the cell surface sialoglycolipids (gangliosides).
D000818:Animals; D004136:Dinitrobenzenes / Q000276:immunology; D005732:Gangliosides / Q000276:immunology; D006017:Glycolipids / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008563:Membrane Lipids / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D012795:Sialoglycoproteins / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D004136:Dinitrobenzenes; D005732:Gangliosides; D006017:Glycolipids; D008563:Membrane Lipids; D008565:Membrane Proteins; D012795:Sialoglycoproteins; C009255:sialoglycolipids