Determination of coumarin anticoagulant rodenticide residues in animal tissue by high-performance liquid chromatography. II. fluorescence detection using ion-pair chromatography.
A high-performance liquid chromatographic method was developed for the determination of warfarin, coumatetralyl, bromadiolone, difenacoum and brodifacoum in animal tissues using fluorescence detection. Ion-pair chromatography, with the tetrabutylammmonium ion as counter-ion, was used to take full advantage of their native fluorescence. Detection limits in liver tissue after gel permeation clean-up were 0.002 mg kg-1 for coumatetralyl, difenacoum and bromdifacoum, 0.008 mg kg-1 for bromadiolone, and 0.01 mg kg-1 for warfarin.
D000818:Animals; D002415:Cats; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003374:Coumarins / Q000032:analysis*; D004285:Dogs; D010573:Pesticide Residues / Q000032:analysis*; D012378:Rodenticides / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods
D003374:Coumarins; D010573:Pesticide Residues; D012378:Rodenticides