Immunoelectron microscopic localization of cathepsin D in lysosomes of rat nerve cells.
Immunoelectron microscopic localization of cathepsin D in rat nerve cells was investigated using protein A-gold technique. Brain and spinal cord were fixed by perfusion with 4% paraformaldehyde and 1% glutaraldehyde in 0.05 M cacodylate buffer. Vibratome sections of the cerebellum and spinal cord were embedded in Epon 812 or Lowicryl K4M. The postembedding immunocytochemical procedures with protein A-gold were applied to ultrathin sections. Gold particles representing the antigen sites of cathepsin D were localized in lysosomes of Purkinje cells and of presumed motorneurons in the anterior horn. A few gold particles were in Golgi stacks of these cells. The results indicate that the main subcellular domain for cathepsin D in rat nerve cells is lysosome.
D000818:Animals; D002402:Cathepsin D; D002403:Cathepsins / Q000032:analysis*; D003594:Cytoplasmic Granules / Q000201:enzymology; D006046:Gold; D006651:Histocytochemistry; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D008854:Microscopy, Electron; D009046:Motor Neurons / Q000201:enzymology* / Q000648:ultrastructure; D011689:Purkinje Cells / Q000201:enzymology; D051381:Rats; D013116:Spinal Cord / Q000201:enzymology*; D013205:Staphylococcal Protein A
D013205:Staphylococcal Protein A; D006046:Gold; D002403:Cathepsins; D002402:Cathepsin D