Oxygen consumption rate as a measurement of freeze-induced damage to chicken spermatozoa.
The present study was conducted to determine if oxygen consumption rate of postthaw spermatozoa could be used as the indicator of freeze-induced damage. Pooled semen was diluted fourfold with 5.7% glucose solution containing various levels of glycerol or N-methylformamide for freezing by the pelleting method. Sperm motility and oxygen consumption rate were determined after thawing the samples at 37 C. 1) The maximum motility and oxygen consumption rate of postthaw spermatozoa were observed at 10 and 7% glycerol, respectively. However, no significant differences existed among semen samples diluted in 7, 10, and 15% glycerol. 2) The motility and oxygen consumption rate of spermatozoa frozen with 6 to 9% of N-methylformamide were higher than those of spermatozoa frozen with 0, 3, 12, or 15%. 3) A highly significant positive correlation was observed between motility and oxygen consumption rate immediately after thawing and also after 10 min incubation at 20 C with either cryoprotectant. 4) The polarographic measurement of oxygen consumption rate of postthaw spermatozoa appeared to be a reliable method for evaluating freeze-induced damage to chicken spermatozoa.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002645:Chickens; D003451:Cryoprotective Agents / Q000494:pharmacology; D005559:Formamides / Q000494:pharmacology; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D012662:Semen Preservation / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*
D000970:Antineoplastic Agents; D003451:Cryoprotective Agents; D005559:Formamides; D005990:Glycerol; C002950:methylformamide