Vitreous fluorophotometry for clinical research. I. Description and evaluation of a new fluorophotometer.
In vitro and in vivo tests of a new commercial fluorophotometer showed that the instrument is capable of measuring fluorescein concentrations of 1 to 1,000 ng/mL accurately. The in vivo estimate of the spread functions of the lens and chorioretinal peaks yielded values of about 1% and 0.5% 3 and 6 mm, respectively, from the peak. Pigmentation did not influence the chorioretinal spread function. Reproducibility in vivo was evaluated to be 12%. Furthermore, when compared with other existing fluorophotometers, the new instrument performed superiorly.
D002829:Choroid; D006801:Humans; D007908:Lens, Crystalline; D012160:Retina; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D014822:Vitreous Body