A reproducible technique for breaking glass micropipettes over a wide range of tip diameters.
A new simple technique for the accurate modification of microelectrode and micropipette tips by breaking has been developed. The breaking is done by longitudinally inserting a micropipette into a larger previously broken pipette for stability and by applying a force on the micropipette to cause it to break. Tips will break cleanly at an easily predetermined diameter over a range of 3-200 micrometers.
D000818:Animals; D003055:Cochlear Microphonic Potentials; D003056:Cochlear Nerve / Q000502:physiology; D004558:Electric Stimulation / Q000295:instrumentation; D008839:Microelectrodes; D009482:Neurophysiology / Q000295:instrumentation*