Quantitative evaluation of the spermicidal potencies of vaginal contraceptives with a transmembrane migration method.
The spermicidal potencies of four vaginal contraceptives, namely Delfen cream, Samtol tablet, Lorophyn suppository and Ortho-Gynol jelly were compared using a transmembrane migration method which measured the proportion of sperm that moved from a semen-drug mixture across a Nuclepore membrane with 5 micron pores into phosphate buffer. The concentration-response curves of all preparations were parallel. The maximal dilutions that decreased sperm motility to 50% of control for Delfen, Samtol, Lorophyn and Ortho-Gynol were 240, 160, 75 and 35 respectively. The trans-membrane migration method is a simple, reproducible and quantitative test for evaluating the spermicidal potencies of vaginal contraceptives.
D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008567:Membranes, Artificial; D013078:Sperm Immobilizing Agents / Q000008:administration & dosage / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects*; D013089:Spermatocidal Agents / Q000008:administration & dosage / Q000494:pharmacology*; D014622:Vaginal Creams, Foams, and Jellies / Q000008:administration & dosage / Q000494:pharmacology*
D008567:Membranes, Artificial; D013078:Sperm Immobilizing Agents; D013089:Spermatocidal Agents; D014622:Vaginal Creams, Foams, and Jellies