title: Changes in some rat hepatic microsomal components induced by prolonged administration of chloroquine.
abstract: Alterations in microsomal drug metabolizing enzymes and phospholipids following prolonged exposure to chloroquine have been investigated. The levels of microsomal aminopyrine-N-demethylase, aniline hydroxylase and both microsomal and cytosolic glutathione-S-transferase are reduced in treated rats. Microsomal epoxide hydrolase is unaffected by the treatment. An increase in the cholesterol-phospholipid ratio and a decrease in the phosphocholine-sphingomyelin ratio occur. There is a general reduction in the total microsomal phospholipid level though the percentage content of sphingomyelin is higher in all cases. The reduction in microsomal phospholipid level is probably due to a reduced incorporation of acetate into microsomal phospholipids as shown in this study using radioactive sodium acetate.
mesh_terms: D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D000815:Aniline Hydroxylase / Q000378:metabolism; D000818:Animals; D002738:Chloroquine / Q000494:pharmacology*; D002784:Cholesterol / Q000032:analysis; D003600:Cytosol / Q000201:enzymology; D004851:Epoxide Hydrolases / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008055:Lipids / Q000032:analysis; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D008055:Lipids; D010743:Phospholipids; D002738:Chloroquine; D002784:Cholesterol; D000815:Aniline Hydroxylase; D000633:Aminopyrine N-Demethylase; D005982:Glutathione Transferase; D004851:Epoxide Hydrolases