A simple method for testosterone measurement by high-performance liquid chromatography-UV spectrophotometer.
A simple, rapid, and reliable method for the separation and quantitation of testosterone in biological samples by a high-performance liquid chromatography-UV spectrophotometer has been developed. Separation of testosterone and prednisolone (I.S.) by HPLC was achieved in less than 3 min on a micro silica gel column using isocratic elution with hexane:chloroform:methanol (3:1:1) as the mobile phase. As little as 5 ng testosterone in biological samples can be estimated accurately by this method. This method seems to provide a new technique for the study of endocrinological pharmacology in animals and human.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D011239:Prednisolone / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet; D013739:Testosterone / Q000032:analysis* / Q000652:urine
D013739:Testosterone; D011239:Prednisolone