Occurrence of saxitoxin and other toxins in the liver of the pufferfish Takifugu pardalis.
Highly toxic livers of the pufferfish Takifugu pardalis were extracted with acidic ethanol. The toxins extracted were partially purified by chromatography on Bio-Gel P-2 and then Bio-Rex 70, resulting in separation into three fractions I, II and III. ratios of total mouse units per fraction were approximately 0.1:100:0.01, respectively, with tetrodotoxin (TTX) as standard. By TLC, electrophoresis and a TTX analyzer, Fr. II was identified as TTX and, unexpectedly, Fr. III as saxitoxin, while Fr. I remains unidentified.
D000818:Animals; D002850:Chromatography, Gel; D004588:Electrophoresis, Cellulose Acetate; D005399:Fishes; D008099:Liver / Q000032:analysis*; D008387:Marine Toxins / Q000032:analysis*; D012530:Saxitoxin / Q000032:analysis*
D008387:Marine Toxins; D012530:Saxitoxin