Evaluation of cytostatic drug concentrations in the kidney, bladder wall, and prostate by means of the diffusion chamber technique in dogs.
The usefulness of a diffusion chamber model for measurement of concentrations of cytostatics in the interstitial fluid of tissues was tested. Chambers (pore size of the membranes: 0.45 mcm) were implanted in the kidney, bladder wall, and prostate of dogs. The concentrations after application of Doxorubicin-hydrochloride (2 mg/kg body wt.) and high doses of Methotrexate (100 mg/kg body wt.) were measured simultaneously and continuously in serum and in the above organs. The investigations showed that knowledge of the plasma level alone does not permit a prediction of the concentrations in the organs to be made. The diffusion chamber technique proved itself to be relatively simple to perform, economical, and it provides reproducible values.
D000818:Animals; D004285:Dogs; D004317:Doxorubicin / Q000378:metabolism*; D005110:Extracellular Space / Q000032:analysis; D006207:Half-Life; D007668:Kidney / Q000032:analysis*; D007700:Kinetics; D008297:Male; D008727:Methotrexate / Q000378:metabolism*; D011467:Prostate / Q000032:analysis*; D019736:Prostheses and Implants; D014018:Tissue Distribution; D001743:Urinary Bladder / Q000032:analysis*
D004317:Doxorubicin; D008727:Methotrexate