Differential effects of amorphous and crystalline nickel sulfide on murine alpha/beta interferon production.
Mouse embryo fibroblast cultures were exposed to various concentrations of amorphous or crystalline NiS (nickel sulfide). After removal of the NiS from the cultures, polyriboinosinic-polyribocytidylic acid was added to induce alpha/beta interferon. Pretreatment of the cultures with crystalline NiS significantly inhibited the production of alpha/beta interferon at relatively noncytotoxic concentrations (i.e., 1 microgram/ml) while amorphous NiS had no significant effect on alpha/beta interferon production at 10 micrograms/ml. Treatment of cells with reduced glutathione did not block the inhibitory effects of crystalline NiS on alpha/beta interferon production, suggesting that these effects are not mediated by a free-radical mechanism.
D000818:Animals; D002478:Cells, Cultured; D003460:Crystallization; D005347:Fibroblasts; D005978:Glutathione / Q000494:pharmacology; D007370:Interferon Type I / Q000096:biosynthesis*; D051379:Mice; D008809:Mice, Inbred C3H; D009532:Nickel / Q000494:pharmacology*
D007370:Interferon Type I; D009532:Nickel; D005978:Glutathione; C017558:nickel sulfide