A method of DNA histogram analysis by computer and its evaluation by simultaneous combination with 3H-thymidine autoradiography.
A method for determining the fractions of cells in the G1, S, and G2 + M phases of the cell life cycle, by quantifying DNA histograms derived from static fluorescence cytophotometry, was evaluated by simultaneous combination with 3H-thymidine autoradiography. DNA histograms were obtained by cytofluorometry on the Feulgen-stained autoradiographs of HeLa cells, and mouse and rat hepatocytes, after DNA labelling with 3H-thymidine. The synthetic histogram determined by "sum of discrete normal curves" technique was fitted to the experimental data according to a weighted least-squares method by a desk-top computer (HP 85F). The mean relative percent deviations of estimated cell cycle phase fractions from the actual phase fractions determined directly on an autoradiograph was 6.6 +/- 3.3%.
D000375:Aging; D000818:Animals; D001345:Autoradiography; D002417:Cattle; D002453:Cell Cycle; D002467:Cell Nucleus / Q000032:analysis*; D003201:Computers; D004247:DNA / Q000032:analysis*; D005260:Female; D005333:Fetus; D005434:Flow Cytometry / Q000379:methods; D006367:HeLa Cells / Q000648:ultrastructure; D008099:Liver / Q000648:ultrastructure*; D051379:Mice; D011247:Pregnancy; D051381:Rats; D013936:Thymidine; D014316:Tritium
D014316:Tritium; D004247:DNA; D013936:Thymidine