[Comparative study of the phospholipase activity of pathogenic and saprophytic Leptospira cultured on a serum-lecithin agar].
The phospholipase activity of leptospires cultivated on serum-lecithin agar has been studied. Two zones of changes in the medium have been found to appear around the colonies of saprophytic Leptospira strains: transparent (5.25 +/- 2.09 mm wide) and turbid (6.90 +/- +/- 1.46 mm wide), which is linked with the production of phospholipases A and C. Only a single clear zone is formed around the colonies of pathogenic strains due to the production of phospholipase A. At the same time virulent Leptospira strains show greater phospholipase activity (the zones are 6.0 +/- 1.2 mm wide) than avirulent strains (the zones are 1.6 +/- +/- 0.04 mm wide).
D000362:Agar / Q000378:metabolism; D000818:Animals; D006224:Cricetinae; D003470:Culture Media / Q000378:metabolism*; D007921:Leptospira interrogans / Q000201:enzymology* / Q000472:pathogenicity; D008647:Mesocricetus; D010713:Phosphatidylcholines / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D014774:Virulence
D003470:Culture Media; D010713:Phosphatidylcholines; D000362:Agar; D010740:Phospholipases
Sravnitel'noe izuchenie fosfolipazmoĭ aktivnosti patogennykh i saprofitnykh leptospir pri kul'tivirovanii na syvorotochno-letsitinovom agare.