A novel combination of techniques for the assay of lactate dehydrogenase isoenzymes in plasma and red blood cell lysates.
A method is presented for the preparation and assay of lactate dehydrogenase isoenzymes 1 and 2 as pure fractions. The total method involves the use of heat treatment to destroy the heat-labile fractions (isoenzymes 3, 4 and 5); fractionation using an ion-exchange resin; and immunochemical blocking of the muscle subunit, to remove any contamination of the fraction containing isoenzyme 1 with isoenzyme 2 and vice versa. Finally, the enzyme activities in the various fractions are measured using a standard kinetic assay procedure. Estimates can be obtained of all five isoenzymes if the heat treatment step is omitted.
D000328:Adult; D000368:Aged; D000906:Antibodies; D002852:Chromatography, Ion Exchange; D004586:Electrophoresis; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D006461:Hemolysis; D006358:Hot Temperature; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000097:blood* / Q000276:immunology; D008297:Male; D008722:Methods; D008875:Middle Aged; D010949:Plasma / Q000201:enzymology*
D000906:Antibodies; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase