Microfluorometric studies on chromosomes. Quantitative determination of protein content of Chinese hamster chromosome 1 in situ with and without trypsin digestion.
The fluorescent stains, dansyl chloride and fluorescamine, were used to indicate the amount of protein in G-banded and unbanded chromosomes 1 of the Chinese hamster relative to the average amount of protein in human erythrocyte. Fluorescence was found to be proportional to protein mass up to the equivalent of three erythrocytes. In G-banding, trypsin digestion resulted in an average protein loss of 35.4% compared with unbanded chromosomes.
D000818:Animals; D002460:Cell Line; D002871:Chromosome Banding; D002875:Chromosomes / Q000648:ultrastructure*; D002877:Chromosomes, Human / Q000648:ultrastructure; D006224:Cricetinae; D003412:Cricetulus; D003619:Dansyl Compounds; D004912:Erythrocytes / Q000166:cytology; D005450:Fluorescamine; D006801:Humans; D008168:Lung; D008856:Microscopy, Fluorescence; D014357:Trypsin
D003619:Dansyl Compounds; D005450:Fluorescamine; D014357:Trypsin; C029038:dansyl chloride