Immobilized methylglyoxal-bis(guanylhydrazone) induces starfish oocyte maturation.
Methylglyoxal-bis(guanylhydrazone) diHCl (MGBG), an inhibitor of S-adenosylmethionine decarboxylase, was found to induce starfish oocyte maturation at concentrations above 30 microM. Among several analogs of MGBG three induce oocyte maturation and one lacks the maturation-inducing activity while possessing the S-adenosylmethionine decarboxylase-inhibiting activity. Although MGBG is required during a slightly longer period than the natural hormone 1-methyladenine (1-MeAde), the maturation kinetics are identical. MGBG-induced maturation is sensitive to the same inhibitors as 1-MeAde-induced maturation (theophylline, caffeine, procaine, nicotine, NH4Cl, dansylcadaverine, vinblastine, R24571, and trifluoperazine). Inhibition is reversed by increasing the MGBG concentration. MGBG also induces an increase of protein phosphorylation. MGBG and 1-MeAde were separated on the basis of charcoal adsorption, MgSO4 precipitation, and thin-layer chromatography. MGBG covalently linked to CH-Sepharose 4B induces maturation in oocytes whose jelly layer and vitelline coat have been removed by a moderate pronase treatment, but not in the untreated oocytes. The MGBG-CH-Sepharose 4B beads come in close contact with the plasma membrane only in the pronase-treated oocytes. The mode of action of MGBG and the implications of these results in the purification of the 1-MeAde receptor are discussed.
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D012437:Adenosylmethionine Decarboxylase / Q000037:antagonists & inhibitors; D000818:Animals; D005260:Female; D006146:Guanidines / Q000494:pharmacology*; D008540:Meiosis / Q000187:drug effects; D008935:Mitoguazone / Q000494:pharmacology*; D009865:Oocytes / Q000187:drug effects*; D009866:Oogenesis / Q000187:drug effects*; D010750:Phosphoproteins / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism; D012995:Solubility; D013215:Starfish / Q000187:drug effects*; D013329:Structure-Activity Relationship
D006146:Guanidines; D010750:Phosphoproteins; D011073:Polyamines; C008407:1-methyladenine; D012437:Adenosylmethionine Decarboxylase; D000225:Adenine; D008935:Mitoguazone