The estimation of host antigen in experimental and commercial influenza subunit preparations by delayed-type hypersensitivity reaction.
A delayed-type hypersensitivity (DTH) test has been used to detect the presence of host-specific antigen(s) in highly-purified egg-grown influenza virus and virus components. Cyclophosphamide-treated mice, primed with an extract from allantoic fluid, showed DTH reactions when challenged with whole virus, spikeless virus particles or polymer-type subunits. All commercial egg-grown influenza vaccines showed the presence of host antigen, whereas lipid-free derivatives such as internal virus protein complexes and monomer HA preparations lacked the activity. These findings are discussed in relation to vaccine application.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D003429:Cross Reactions; D006968:Hypersensitivity, Delayed / Q000276:immunology* / Q000473:pathology; D007252:Influenza Vaccines / Q000032:analysis*; D051379:Mice; D013997:Time Factors
D000956:Antigens, Viral; D007252:Influenza Vaccines