[Age characteristics of the interaction between fluorescent probes and thyroxine with the membranes of rat liver microsomes].
By means of fluorescent probes, 1-anilino naphthalene-8-sulfonate (ANS), methoxybenzanthrone (MBA) and pyrene, an increase of membrane affinity to ANS, shift of MBA fluorescence maximum to the short-wave spectrum region and a change of the membrane microviscosity were observed in the liver microsome membranes during aging of rats. No significant changes of fluorescent parameters of the probes with rats aging were found in mitochondria. ANS was concurrently displaced by thyroxine, affinity to which significantly increased with aging, and in young animals during hyperthyroidism. The increase of microsome membranes affinity to thyroxine with age is considered as an intracellular mechanism which is involved in the metabolism changes of hypothyroid pattern in rat liver during aging.
D000345:Affinity Labels; D000375:Aging; D000817:Anilino Naphthalenesulfonates; D000818:Animals; D001551:Benz(a)Anthracenes; D001667:Binding, Competitive; D005456:Fluorescent Dyes; D006980:Hyperthyroidism / Q000378:metabolism / Q000503:physiopathology; D007425:Intracellular Membranes / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism* / Q000648:ultrastructure; D008930:Mitochondria, Liver / Q000378:metabolism / Q000648:ultrastructure; D011721:Pyrenes; D051381:Rats; D011919:Rats, Inbred Strains; D013974:Thyroxine / Q000378:metabolism*
D000345:Affinity Labels; D000817:Anilino Naphthalenesulfonates; D001551:Benz(a)Anthracenes; D005456:Fluorescent Dyes; D011721:Pyrenes; C015345:methoxybenzanthrone; D013974:Thyroxine
Vozrastnye osobennosti vzaimodeĭstviia fluorestsentnykh zondov i tiroksina s membranami mikrosom pecheni krys.