title: Structural changes in nerve endings of rat median eminence superfused with media rich in potassium ions.
abstract: In vitro fragments of male rat mediobasal hypothalami were superfused with Krebs--Ringer solution in the presence or absence of CaCl2. Infusions containing up to 60 mM potassium chloride were applied, at the end of which tissues were fixed in osmium tetroxide and prepared for transmission electron microscopy. Control superfusions were run in parallel. Quantitative measurements performed on electron micrographs of the outermost palisade region showed significant (20-30%) increase in caliber of axon endings after intensive potassium ion stimulation. Ultrastructurally, widespread depletion of granular vesicles and microvesicles was found. Vesicle shift to the outer zone of the terminals, formation of membrane-bound tubules of the same diameter as microvesicles, and images of attachment and collapse of vesicles into the axolemma were found, particularly after 1 min stimulation. These findings were interpreted as consistent with exocytosis. Longer stimulations were followed by the appearance of large pleomorphic vacuoles that are probably the result of post-exocytotic membrane retrieval. Axon enlargement and vesicle depletion were absent in specimens superfused with calcium-free medium containing high potassium. The functional significance of these ultrastructural changes are interpreted as supporting the hypothesis that exocytosis of calcium-loaded microvesicles can contribute to extrude this ion from median eminence nerve endings during secretion.
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003470:Culture Media; D066298:In Vitro Techniques; D008297:Male; D008473:Median Eminence / Q000187:drug effects* / Q000648:ultrastructure; D009411:Nerve Endings / Q000187:drug effects* / Q000648:ultrastructure; D011188:Potassium / Q000494:pharmacology*; D051381:Rats / Q000502:physiology*; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical
chemical_list: D003470:Culture Media; D011188:Potassium; D002118:Calcium