title: Defined minimal growth medium for Acanthamoeba polyphaga.
abstract: Nutritional requirements of Acanthamoeba polyphaga (strain PD) were compared to those reported for A. castellanii. Although A. polyphaga and A. castellanii have essentially the same minimal amino acid requirements--arginine, methionine, leucine, isoleucine, and valine--A. polyphaga cannot utilize acetate as sole carbon source, but A. castellanii can if the medium is supplemented with glycine.
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000596:Amino Acids / Q000494:pharmacology*; D000656:Amoeba / Q000254:growth & development* / Q000378:metabolism; D000818:Animals; D003470:Culture Media; D005947:Glucose / Q000378:metabolism*; D005998:Glycine / Q000494:pharmacology
chemical_list: D000085:Acetates; D000596:Amino Acids; D003470:Culture Media; D005947:Glucose; D005998:Glycine