High performance liquid chromatographic analysis of cholesterol in milk.
A method was developed for analysis of cholesterol in whole milk powder and commercial liquid milk products. High performance liquid chromatography employed a nonaqueous reversed phase chromatographic system. Sample preparation was by saponification with methanolic potassium hydroxide followed by extraction with ether. Potential interfering substances were removed prior to injection by a simple clean-up procedure using a silica gel Sep-pakTM. The chromatographic system included a carbon-18 column with a hexane/isopropanol mobile phase and detection at 205 nm. Method validation and recovery studies using carbon-14 labeled cholesterol indicated excellent performance.
D000818:Animals; D002417:Cattle; D002784:Cholesterol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D008892:Milk / Q000032:analysis*
D002784:Cholesterol