title: Chronic relapsing experimental allergic encephalomyelitis. The presence in the cerebrospinal fluid of factors chemotactic for monocytes.
abstract: Cerebrospinal fluid (CSF) was removed from guinea pigs with chronic relapsing experimental allergic encephalomyelitis (CR-EAE) and control inoculated animals by puncture of the cisterna magna. The fluid from 7/8 animals in relapse and 2/4 animals in remission phases of CR-EAE was found to promote the migration of peripheral blood monocytes through a 5-micron pore diameter polycarbonate membrane filter. Monocytes were also found to orient towards the migration-stimulating CSF in a gradient formed between such fluid and CSF derived from a control animal, thereby indicating the presence of a chemotactic factor. The factor responsible for promoting monocyte migration had a molecular weight of between 50 000 and 300 000 as defined by ultrafiltration. The results are discussed in relation to the known pathohistological features of the chronic relapsing disease.
mesh_terms: D000818:Animals; D002555:Cerebrospinal Fluid / Q000032:analysis / Q000276:immunology*; D002630:Chemotactic Factors / Q000032:analysis / Q000276:immunology*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D006168:Guinea Pigs; D007249:Inflammation / Q000276:immunology; D009000:Monocytes / Q000032:analysis / Q000276:immunology*
chemical_list: D002630:Chemotactic Factors