title: Ovarian and plasma PGE and PGF levels in naturally ovulating brook trout (Salvelinus fontinalis) and the effects of indomethacin on prostaglandin levels.
abstract: Plasma and ovarian levels of PGE and PGF were measured by radioimmunoassay in naturally ovulating brook trout (Salvelinus fontinalis). At 12 hours post-ovulation, PGF levels in plasma and ovarian tissue were significantly elevated over levels in gravid controls. Plasma PGF levels were still elevated 5-7 days post-ovulation. Indomethacin treatment (10 micrograms/gm body weight) of ovulatory brook trout significantly decreased plasma and ovarian PGF levels by 12 hours post-ovulation. No significant differences were observed in plasma PGE levels, though gravid controls contained significantly elevated ovarian PGE levels as compared to ovulated fish.
mesh_terms: D000818:Animals; D005260:Female; D007213:Indomethacin / Q000494:pharmacology*; D010053:Ovary / Q000378:metabolism; D010060:Ovulation; D011458:Prostaglandins E / Q000097:blood / Q000378:metabolism*; D011460:Prostaglandins F / Q000097:blood / Q000378:metabolism*; D012487:Salmonidae / Q000378:metabolism*; D014337:Trout / Q000378:metabolism*
chemical_list: D011458:Prostaglandins E; D011460:Prostaglandins F; D007213:Indomethacin