Comparative assays for doxepin and desmethyldoxepin using high-performance liquid chromatography and high-performance thin-layer chromatography.
Two chromatographic methods, high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) were compared for sensitivity and reproducibility in the analysis of the tricyclic antidepressant doxepin and its metabolite, desmethyldoxepin, in plasma. The HPLC procedure yielded a better reproducibility, as reflected by the coefficient of variation, and a higher sensitivity, as reflected by the minimum detectable quantity. The application of these methods for therapeutic and subtherapeutic monitoring of plasma levels of the drug is described.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D004285:Dogs; D004316:Doxepin / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D013997:Time Factors
D004316:Doxepin; C028701:desmethyldoxepin