Correlation between endogenous nucleosomal hyper(ADP-ribosyl)ation of histone H1 and the induction of chromatin relaxation.
The effect of poly(ADP-ribose) synthesis on chromatin structure was investigated by velocity sedimentation and electron microscopy. We demonstrate that locally relaxed regions can be generated within polynucleosome chains by the activity of their intrinsic poly(ADP-ribose)polymerase. This relaxation phenomenon is also shown to be NAD dependent and to be correlated with the formation of hyper(ADP-ribosyl)ated forms of histone H1. Evidence is also presented which suggests that hyper(ADP-ribosyl)ated histone H1 is neither released from the relaxed chromatin, nor does it seem to participate in polynucleosomal aggregation.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D002458:Cell Fractionation; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure*; D006657:Histones / Q000378:metabolism*; D008836:Micrococcal Nuclease; D008854:Microscopy, Electron; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D009707:Nucleosomes / Q000378:metabolism* / Q000648:ultrastructure; D010179:Pancreas / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats
D002843:Chromatin; D006657:Histones; D009702:Nucleoside Diphosphate Sugars; D009707:Nucleosomes; D000246:Adenosine Diphosphate Ribose; D008836:Micrococcal Nuclease