Genetic stability of Newcastle disease virus CG179: a strain containing only clear plaque subpopulations.
Two cultures of the CG179 strain of Newcastle disease virus were maintained apart in two laboratories for over 30 years. One culture had been passaged 45 times through embryonated chicken eggs, yet this virus had the same virulence, thermostability of hemagglutinin, plaque types, and RNA fingerprint as a culture lyophilized in 1948. The genetic stability observed here has not been reported for strains containing both red and clear plaques and may occur only in strains like CG179 that do not have red plaques.
D000818:Animals; D002642:Chick Embryo; D046508:Culture Techniques; D005347:Fibroblasts; D006389:Hemagglutinins, Viral / Q000032:analysis; D009522:Newcastle disease virus / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D009841:Oligonucleotides / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D014774:Virulence; D014776:Virus Cultivation
D006389:Hemagglutinins, Viral; D009841:Oligonucleotides; D012367:RNA, Viral