A modified screening method for rapid simultaneous determination of dopamine, noradrenaline and serotonin in the same brain region.
A modification of previously published fluorimetric methods for brain noradrenaline (NA), dopamine (DA), and serotonin (5-HT) assay is presented in this paper. The modification improved the sensitivity to 5-HT and resulted in a less time-consuming and less expensive method for noradrenaline and dopamine determination. The assay can be used for simultaneous estimation of NA, DA and 5-HT as well as for turnover studies, utilizing catecholamine synthesis inhibition or monoaminoxidase inhibition.
D000818:Animals; D001923:Brain Chemistry; D004298:Dopamine / Q000032:analysis*; D008297:Male; D051379:Mice; D009638:Norepinephrine / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods
D012701:Serotonin; D004298:Dopamine; D009638:Norepinephrine