[Effects of sodium molybdate on molecular forms of steroid receptors in human breast tumors. The value of high performance liquid chromatography (HPLC)].
Estrogen, progesterone and glucocorticoid receptors from 124 breast cancer cytosols were studied by high performance liquid chromatography (HPLC), in comparison with sephadex chromatography and density gradient ultracentrifugation. Sodium molybdate was used as a receptor-hormone complex stabilizer, and as a tool allowing to study the different molecular forms of the receptors. In the presence of molybdate during the entire saturation process, more receptor sites were detected, without modification of equilibrium reaction kinetics. HPLC receptors analysis was rapid, with an high relative separation efficiency. Multiple molecular forms of steroid receptors were observed: five forms each for progesterone and glucocorticoid receptors, and six forms for estrogen receptors. The presence or absence of one of these forms emphasized the biological variability of the tumors and provides further questions or hormone-dependence.
D001943:Breast Neoplasms / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008968:Molecular Conformation; D008982:Molybdenum / Q000494:pharmacology*; D011987:Receptors, Steroid / Q000032:analysis / Q000187:drug effects*
D011987:Receptors, Steroid; D008982:Molybdenum; C024687:sodium molybdate(VI)
Effets du molybdate de sodium sur les formes moléculaires de récepteurs de stéroïdes des tumeurs mammaires humaines. Intérêt de la chromatographie liquide à haute performance (HPLC).