Isolation of low molecular protein P-9 from nuclei of NK/Ly lymphoma and Guerin carcinoma.
Protein with molecular weight 9000 (protein-9) was found in nuclei of resting cells of NK/Ly lymphoma and Guerin carcinoma by electrophoresis in polyacrylamide gel. Method of preparative isolation P-9 concluding in fractionation of nuclei from phenol extract by acetone at different pH and following electrophoresis in polyacrylamide gel was made. Electrophoretically homogeneous preparation of protein P-9 from nuclei of NK/Ly lymphoma and Guerin carcinoma was obtained.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002467:Cell Nucleus / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D008223:Lymphoma / Q000032:analysis*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000302:isolation & purification*; D009374:Neoplasms, Experimental / Q000032:analysis*; D051381:Rats
D000596:Amino Acids; D009363:Neoplasm Proteins