On-line diode array UV-visible spectrometry in screening for drugs and drug metabolites by high-performance liquid chromatography.
The direct coupling of a multi-channel diode array UV-visible spectrophotometer to a powerful reversed-phase HPLC separation system is considered, especially for use in qualitative analysis, e.g., screening/identification of drugs and drug metabolites. The approach is illustrated by the screening for metabolites of butoprozine and ticlopidine directly in human and rat bile.
D000638:Amiodarone / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D001646:Bile / Q000032:analysis; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D006801:Humans; D008297:Male; D008875:Middle Aged; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry / Q000295:instrumentation; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation; D013876:Thiophenes / Q000378:metabolism; D013988:Ticlopidine
D004364:Pharmaceutical Preparations; D013876:Thiophenes; C014921:butoprozine; D000638:Amiodarone; D013988:Ticlopidine