Electrophoretic study on the DNA-breaking actions of ascorbic acid and triose reductone in the presence of Cu2+.
The mode of DNA-breaking actions of ascorbic acid (AsA) and triose reductone (TR) in the presence of Cu2+ was studied by use of 0.8% agarose slab gel electrophoretic analysis. The DNA-breaking actions of the mixture of AsA or TR and Cu2+ were inhibited by N2 gas, hydroxyl radical scavengers and catalase, indicating that the oxidation of AsA or TR by Cu2+ and the hydrogen peroxide and hydroxyl radicals resulting from the oxidation are essential for the fragmentation of DNA by the mixture of AsA or TR and Cu2+. However, the DNA-breaking activity of these reductones varied with pH, while their oxidation rates were proportional to the increase in pH. The marked fragmentation of DNA occurred at pH 4, 7 or 8. The breakages of DNA by AsA or TR in the presence of Cu2+ were concluded not to be associated with the oxidation rates of reductones.
D000818:Animals; D001205:Ascorbic Acid; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D003300:Copper; D004247:DNA; D004587:Electrophoresis, Agar Gel; D005609:Free Radicals; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008314:Malonates; D008315:Malondialdehyde / Q000031:analogs & derivatives*; D010084:Oxidation-Reduction; D013950:Thymus Gland
D005609:Free Radicals; D008314:Malonates; C010778:reductone; D008315:Malondialdehyde; D003300:Copper; D004247:DNA; D001205:Ascorbic Acid