Preparation of microspheres of poly(glycidyl methacrylate) and its derivatives as carriers for immobilized proteins.
Microspheres of poly(glycidyl methacrylate) and its derivatives have been prepared. The diameter can be varied in the range from 0.5 to 5 micron. Proteins were immobilized on the microspheres by various processes. The maximum amount of covalently immobilized gamma-globulin on the microspheres was found to be about 6 mg/m2. It was shown that dyed microspheres with a diameter of 2-4 micron are applicable to immunological agglutination tests on a microplate as carrier particles substituting for red blood cells.
D000179:Acrylates; D000818:Animals; D001799:Blood Sedimentation; D002352:Carrier Proteins; D002417:Cattle; D002645:Chickens; D004852:Epoxy Compounds / Q000378:metabolism; D004988:Ethers, Cyclic; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D008689:Methacrylates / Q000378:metabolism; D008863:Microspheres; D011817:Rabbits; D012756:Sheep
D000179:Acrylates; D002352:Carrier Proteins; D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D007074:Immunoglobulin G; D008689:Methacrylates; C007870:glycidyl methacrylate