[Cytoplasmic and nuclear testosterone receptors in the hypothalamus of androgenized female and castrated male rats].
Receptors for testosterone (T) in the preopticoanterior hypothalamus (PO) and in the arcuate nucleus and median eminence (ARC + ME) were examined in neonatally androgenized female and neonatally castrated male rats. As a result of neonatal castration of males, the concentration of cytoplasmic and nuclear receptors for T in the PO dropped to an undetectable level. In the ARC + ME, the number of T-binding sites in the cytosole fraction remained unchanged, while that in the nuclear fraction decreased 2-fold. In the cytosole fraction of neonatally androgenized females, receptors for T were detectable only in the ARC + ME, the level of binding being not different from that seen in this hypothalamic area in intact and neonatally castrated males. At the same time in the nuclear fraction, receptors for T were detectable in both hypothalamic areas, the number of T-binding sites in the ARC + ME being 1.5 times less than in the PO. The data obtained attest to the involvement of receptors for T in sexual differentiation of the brain and regulation of gonadotropic function of the hypophysis.
D000818:Animals; D001665:Binding Sites; D002369:Castration; D002467:Cell Nucleus / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D005260:Female; D007031:Hypothalamus / Q000648:ultrastructure*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011944:Receptors, Androgen / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism*
D011944:Receptors, Androgen; D011987:Receptors, Steroid; D013739:Testosterone
Tsitoplazmaticheskie i iadernye retseptory k testosteronu v gipotalamuse u androgenizirovannykh samok i kastrirovannykh samtsov krys.