[Mechanism of flagellin polymerization in Bacillus brevis].
The optical properties of acid-dissociated and tetranitromethane-modified flagellin has been studied by circular dichroism (CD) techniques. The flagellins have the same CD spectra which do not change over the 2.9-10.0 range. The spectra character depends on pH in the presence of polyethylene glycol (PEG) and ammonium sulphate which accelerate polymerization. As the content of PEG goes up to 20% and ammonium sulphate to 1 M, the value of the molecular ellipticity at 222 nm ([O]222) of the both flagellins considerably increases at pH 4.3-10.0, however [O]222 does not achieve the value for bacterial flagella. PEG and ammonium sulphate addition at pH 2.9-3.9 gives less dramatic increase of the [O]222 value. It has been concluded that the changes in the CD spectra at pH 4.3-10.0 is a result of conformational rearrangements in flagellin before it incorporates into the flagella.
D001407:Bacillus / Q000187:drug effects / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002942:Circular Dichroism; D005407:Flagella / Q000187:drug effects / Q000378:metabolism; D005408:Flagellin / Q000032:analysis / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D011108:Polymers / Q000032:analysis / Q000378:metabolism*
D001426:Bacterial Proteins; D011108:Polymers; D005408:Flagellin
Izuchenie mekhanizma polimerizatsii flagellina Bacillus brevis.