Enhanced tumorigenicity of cloned UV-regressor tumor lines following selected in vivo and in vitro manipulations.
Passage of cloned ultraviolet (UV) radiation-induced fibrosarcomas with regressor phenotype through 500-rad-irradiated syngeneic mice resulted in their conversion to transplantable progressor tumors. A similar conversion in tumorigenic phenotype (regressor leads to progressor) was found to be inducible in vitro by coculturing a cloned regressor tumor with normal splenocytes, but not with splenocytes from tumor-immune or UV-irradiated animals. Recloning of regressor and converted progressor tumor lines yielded regressor and progressor phenotype subclones, respectively, suggesting a degree of stability in their growth phenotype. Although all of the cloned progressor tumors tested were found to be cross-reactive with related regressor tumor lines, suggesting that related clones share a similar tumor-specific transplantation antigen, the progressor clones appeared to be less immunogenic than the regressor clones. Potential mechanisms that influence this conversion in tumorigenic phenotype are discussed.
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D005260:Female; D005354:Fibrosarcoma / Q000276:immunology*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D009381:Neoplasms, Radiation-Induced / Q000276:immunology*; D010641:Phenotype; D012878:Skin Neoplasms / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D014181:Transplantation Immunology / Q000528:radiation effects; D014185:Transplantation, Isogeneic; D014466:Ultraviolet Rays
D000951:Antigens, Neoplasm