Protein A radio-assay of H-Y antigen on human leukocytes using mouse and rat antisera and monoclonal antibodies.
The presence of H-Y antigen on human leukocytes was investigated using a protein A radio-assay. H-Y antigen could be demonstrated on male cells using either conventional H-Y antisera produced in mice and rats, or monoclonal H-Y antibodies. With mouse antiserum and IgG-type monoclonal antibody the reaction was male-specific using a single antibody. The reaction obtained with rat antiserum was enhanced by the application of a second antibody (rabbit anti-mouse IgG). This technique provides a rapid, simple, objective, and semiquantitative method for the determination of cellular H-Y antigen, the results being expressed as radioactivity bound to the test cells and thus being independent of human observation. It requires only 10-20 ml of blood and small quantities of antiserum or antibody.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D005260:Female; D006182:H-Y Antigen / Q000032:analysis / Q000235:genetics*; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011869:Radioligand Assay; D051381:Rats; D013205:Staphylococcal Protein A
D000911:Antibodies, Monoclonal; D006182:H-Y Antigen; D007106:Immune Sera; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A