Genetic analysis of reactivities of allospecific monoclonal antibodies to rat histocompatibility antigens.
The reactions of a number of LEW anti-ACI allospecific rat monoclonal antibodies were examined in a series of breeding studies. Analysis of (LEW X ACI) F2 progeny demonstrated concordance of the monoclonal class I and class II specificities with results of conventional alloantisera typing. In addition, a "gene dosage" effect was noted, with a/a homozygotes showing approximately twice the reactivity as a/l heterozygotes. The previously noted crossreaction of antibody 36.3 (anti-RT1.Aa) with WF and RP strains segregated with RT1.Au in (WF X ACI) F2 and (LEW X RP) F2 progeny suggesting structural similarity of these two allelic products. Finally, an apparent decreased reaction of two (WF X ACI) F2 offspring with antibody 36.3 was shown to be heritable to the F5 generation and may represent an example of genetic control of level of expression of a class I antigen.
D000483:Alleles; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics*; D000918:Antibody Specificity; D003429:Cross Reactions; D004303:Dosage Compensation, Genetic; D005260:Female; D005838:Genotype; D006649:Histocompatibility Antigens / Q000276:immunology*; D007518:Isoantibodies / Q000235:genetics*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000276:immunology
D000911:Antibodies, Monoclonal; D006649:Histocompatibility Antigens; D007518:Isoantibodies