Enzymatic determination of oxalates.
An assay for oxalate, based on oxalate oxidase, was studied. It is relatively simple, rapid and reproducible and may be semi-automated and is accurate in the range 10-140 mg/l, has a coefficient of variation of 4.2% in the healthy range (10-19 mg/l serum). Samples may be stored at -20 degrees C for as long as 2 weeks. It cannot be used for the determination of urinary oxalate because of the presence of variable amounts of low molecular weight inhibitor(s).
D001322:Autoanalysis; D006801:Humans; D010070:Oxalates / Q000097:blood* / Q000652:urine; D010088:Oxidoreductases / Q000378:metabolism*; D012016:Reference Values; D013053:Spectrophotometry / Q000379:methods
D010070:Oxalates; D010088:Oxidoreductases; C020598:oxalate oxidase