A comparison of Vicia-faba-root S10 and rat-liver S9 activation of ethanol, maleic hydrazide and cyclophosphamide as measured by sister-chromatid exchange induction in Chinese hamster ovary cells.
A comparison of Vicia-faba-root S10 with rat-liver S9 was made in their capacity to bring about, in vitro, the metabolic activation of ethanol, maleic hydrazide (MH) and cyclophosphamide (CP) that can lead to the induction of sister-chromatid exchanges (SCEs) in CHO cells. When NADP was a cofactor in the S9 mix, ethanol, MH and CP all induced an increase of SCEs with rat-liver S9. With Vicia-root S10, however, ethanol induction of SCEs was very weak and no effect at all was observed with MH and CP. When NAD was a cofactor in the S9 mix, Vicia-root S10 activated ethanol and produced an increase in SCEs.
D000818:Animals; D001711:Biotransformation; D002478:Cells, Cultured; D006224:Cricetinae; D003434:Crossing Over, Genetic / Q000187:drug effects*; D003520:Cyclophosphamide / Q000378:metabolism / Q000494:pharmacology; D000431:Ethanol / Q000378:metabolism / Q000494:pharmacology; D008300:Maleic Hydrazide / Q000378:metabolism / Q000494:pharmacology; D008862:Microsomes, Liver / Q000378:metabolism*; D009152:Mutagenicity Tests / Q000379:methods*; D009249:NADP / Q000378:metabolism; D010944:Plants; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D000431:Ethanol; D009249:NADP; D003520:Cyclophosphamide; D008300:Maleic Hydrazide