Mechanism of inhibition by gossypol of glycolysis and motility of monkey spermatozoa in vitro.
Low levels of gossypol inhibited motility and anaerobic glycolysis of ejaculated rhesus monkey spermatozoa. Inhibition (50%) of both occurred at a gossypol: sperm ratio of 8 nmol/10(8) spermatozoa, and complete inhibition of both occurred at a ratio of 75 nmol/10(8) spermatozoa. Determination and comparison of the levels of glycolytic intermediates in intact spermatozoa, incubated with and without gossypol, indicated that the only site of glycolytic inhibition was lactate dehydrogenase-X (EC 1 X 1 X 1 X 27). At gossypol: sperm ratios above 6 nmol/10(8) spermatozoa, gossypol also decreased the concentrations of the adenine nucleotides, ATP, ADP and AMP, and this is most probably the basis for its toxic effect on spermatozoa.
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D003864:Depression, Chemical; D006019:Glycolysis / Q000187:drug effects; D006072:Gossypol / Q000494:pharmacology*; D066298:In Vitro Techniques; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors; D008253:Macaca mulatta; D008297:Male; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism*
D000227:Adenine Nucleotides; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D006072:Gossypol