The effect of bumetanide on the stria vascularis: a stereological analysis of cell volume density.
The purpose of this research was to determine the effect of bumetanide on the volume density (Vv) of the cells, capillaries and intercellular spaces of the stria vascularis (SV). 29 chinchillas were divided into seven groups. There were 3 experimental groups, three control groups and one normal, untreated, group of animals. After either a 20 mg/kg intravenous injection of bumetanide or an injection of a control solution, the animals were killed at 10 min, 1 h and 24 h. One complete radial section of the SV was analyzed in each animal. This section was located at 70% of the length of the basilar membrane as measured from the cochlear apex. Marginal cell volume decreased by 24% and 15% at 10 min and 1 h, respectively, after bumetanide administration. Intermediate cell volume increased by 31% and 27% at 10 min and 1 h, respectively, after bumetanide administration. Intercellular space volume increased by 14% and 21% at 10 min and 1 h, respectively, after bumetanide administration. No significant alteration in the Vv was observed in the strial capillaries or basal cells. A hypothetical model of the ion transporting properties of the SV is presented.
D000818:Animals; D002034:Bumetanide / Q000494:pharmacology*; D002196:Capillaries / Q000187:drug effects; D002452:Cell Count / Q000187:drug effects; D002682:Chinchilla; D003051:Cochlea / Q000187:drug effects*; D004232:Diuretics / Q000494:pharmacology*; D005110:Extracellular Space / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D013316:Stria Vascularis / Q000098:blood supply / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure
D004232:Diuretics; D002034:Bumetanide