Simultaneous determination of total cholesterol concentration and radioactivity in plasma.
Total plasma cholesterol concentration and radioactivity were measured simultaneously using a gas chromatograph equipped with a flame ionization detector and an effluent splitter. More than 99% of the recovered radioactivity was in the cholesterol peak. Specific activities were highly correlated with the amounts of labeled cholesterol present in plasma. The recovery of label was quantitative over a wide range of carrier cholesterol concentrations. The method is highly reproducible, accurate, rapid and specific.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D002784:Cholesterol / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods; D008099:Liver / Q000378:metabolism; D011422:Propionates / Q000378:metabolism; D051381:Rats
D000085:Acetates; D011422:Propionates; D002784:Cholesterol