The effect of primycin on the intracellular monovalent ion and water contents of rat hepatocytes as revealed by energy dispersive X-ray microanalysis and interference microscopy.
Using energy-dispersive X-ray microanalytic and interference microscopic techniques, the intracellular concentration of the monovalent ions (Na+, K+, Cl+) as well as the intracytoplasmic and intracellular water contents were studied in normal and adrenalectomized rat hepatocytes with and without primycin treatment. Although primycin influenced significantly only the intracellular potassium content of the adrenalectomized group, it exerted a marked influence on the intranuclear water content in both the normal and adrenalectomized rats. The intranuclear water content increased significantly in the primycin-treated animals. The conclusion is drawn that the increased level of hydration of the nuclear substances reflects a 'decondensation' of the chromatin which on the other hand, may represent the basis for the various effects of primycin on the induction of certain hepatic enzymes.
D000315:Adrenalectomy; D000818:Animals; D000900:Anti-Bacterial Agents; D002414:Cations, Monovalent; D002451:Cell Compartmentation / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002467:Cell Nucleus / Q000378:metabolism; D004577:Electron Probe Microanalysis; D007476:Ionophores; D007783:Lactones / Q000494:pharmacology; D008099:Liver / Q000187:drug effects*; D018942:Macrolides; D008297:Male; D011188:Potassium / Q000378:metabolism; D051381:Rats; D014867:Water / Q000378:metabolism; D014882:Water-Electrolyte Balance / Q000187:drug effects
D000900:Anti-Bacterial Agents; D002414:Cations, Monovalent; D007476:Ionophores; D007783:Lactones; D018942:Macrolides; D014867:Water; C009041:primycin; D011188:Potassium