Identification of 1-hydroxypyrene as a major metabolite of pyrene in pig urine.
1-Hydroxypyrene is a major metabolite of pyrene given orally to pigs. A method for isolating the metabolite from urine is described, utilizing C18-adsorbent cartridges, h.p.l.c. separation, and fluorescence detection. Unconjugated 1-hydroxypyrene can be detected in the urine of pigs following oral administration of as little as 1 mg pyrene. Identity of the 1-hydroxypyrene was confirmed by u.v.-absorption and fluorescence spectrometry, mass spectrometry, and by comparison with the retention time characteristic of synthetic 1-hydroxypyrene.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D009153:Mutagens / Q000378:metabolism; D011721:Pyrenes / Q000652:urine*; D013053:Spectrophotometry; D013552:Swine
D009153:Mutagens; D011721:Pyrenes; C030984:pyrene; C033146:1-hydroxypyrene