Isolation technique for lymphocytes from bovine lymph glands.
A method is described for obtaining large quantities (grams) of lymphocytes from bovine retropharyngeal lymph glands, whereby contaminating erythrocytes are lysed and coagulation is prevented by the use of acetic acid (0.0143 mol 1(-1). On average 4.38 g (S. D. 1.83, n = 100) lymphocytes, including lymphoblasts, are obtained per lymph gland, with a purity level of about 99%.
D000085:Acetates / Q000494:pharmacology; D019342:Acetic Acid; D000818:Animals; D002417:Cattle; D002469:Cell Separation / Q000379:methods*; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D006461:Hemolysis; D008198:Lymph Nodes / Q000166:cytology*; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D010614:Pharynx; D012996:Solutions
D000085:Acetates; D012996:Solutions; D019342:Acetic Acid