Quantitative studies on sunburn cell formation in mouse epidermis.
Sunburn cell (SC) formation induced by single or combined doses of monochromatic ultraviolet-A (UV-A), ultraviolet-B (UV-B) and ultraviolet-C (UV-C) was studied quantitatively using mouse epidermal sheet preparations (NaBr split, H & E stain). Results were as follows: 1) Action spectra for SC formation showed that wavelengths shorter than 300 nm were most effective. 2) A significant number of SCs was produced when a high dose of UV-A (360 nm) was administered, and it was estimated that approximately 600 times more UV-A (360 nm) energy was required to produce the same number of SCs as was required with UV-B (300 nm). 3) SC formation by UV-B (300 nm) and UV-C (260 nm) showed a logarithm-like dose-response relationship. 4) Time course studies showed that the maximum number of SCs was attained at 18 hr after UV-C (260 nm) irradiation, at 24 hr after UV-B (300 nm) irradiation and at 30 hr after UV-A (360 nm) irradiation. 5) A high dose of UV-A (360 nm) significantly increased the number of SCs induced by UV-B (300 nm) or UV-C (260 nm) when combined with them.
D000818:Animals; D004307:Dose-Response Relationship, Radiation; D008297:Male; D051379:Mice; D012867:Skin / Q000473:pathology / Q000528:radiation effects*; D013471:Sunburn / Q000473:pathology*; D013997:Time Factors; D014466:Ultraviolet Rays