[DNA synthesis in relation to a varying vitamin B12 content in Propionibacterium shermanii cells].
The content of DNA in Propionibacterium cells is nearly twice as low at vitamin B12 deficiency comparing with normal cells. The rate of labeled adenine incorporation into the DNA depends on vitamin B12 content in the cells. The addition of adenosylcobalamin and thymine to the medium makes DNA content rise in the cells of the B12-deficient culture. The addition of thymine to a suspension of B12-deficient cells accelerates the incorporation of labelled adenine into DNA. The authors discuss the phenomenon of bacterial "unbalanced division" and the control of replication by changing the level of DNA precursors.
D000225:Adenine / Q000378:metabolism; D003038:Cobamides / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D004269:DNA, Bacterial / Q000096:biosynthesis*; D011424:Propionibacterium / Q000378:metabolism*; D013941:Thymine / Q000378:metabolism; D014805:Vitamin B 12 / Q000096:biosynthesis*
D003038:Cobamides; D003470:Culture Media; D004269:DNA, Bacterial; C000913:cobamamide; D000225:Adenine; D014805:Vitamin B 12; D013941:Thymine
Sintez DNK v sviazi s razlichnym soderzhaniem vitamina B12 v kletkakh Propionibacterium shermanii.