Quantitation of testolactone and 4,5-dihydrotestolactone in plasma and urine using high-performance liquid chromatography.
A rapid, sensitive, and selective assay is described for the quantitation of both testolactone and its recently identified metabolite, 4,5-dihydrotestolactone, in plasma and urine using high-performance liquid chromatography. The procedure includes a methylene chloride extraction prior to chromatography and quantitation using peak height ratios (ultraviolet absorbance detection, 242 nm) of testolactone and 4,5-dihydrotestolactone to the internal standard, testosterone. A sensitivity of 20 ng/ml for both testolactone and 4,5-dihydrotestolactone is easily achieved using only 0.5 ml of sample. Mean recoveries for testolactone and its metabolite are 95.0% and 81.8%, respectively, and the mean coefficient of variation of the procedure is 3.5% for the drug and 7.1% for the metabolite. This method is currently being used to study the pharmacokinetics of testolactone and 4,5-dihydrotestolactone in male patients. A steady-state plasma concentration versus time profile from a representative patient is included.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008297:Male; D013738:Testolactone / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000652:urine; D013997:Time Factors
C039716:4,5-dihydrotestolactone; D013738:Testolactone