The detection of obligate anaerobic bacteria in swine abscesses. A comparison between gas-liquid chromatography and bacteriological culturing methods.
From the 62 swine abscesses examined, 56 yielded positive cultures, of which 38 cases were mixtures of facultative aerobes and obligate anaerobic bacteria (OAB). Only 6 species appeared to be sterile. In all specimens, which yielded OAB, VFA (C4-C6) could be detected by gas-liquid chromatography (GLC). False negatives were not recorded, and only one false positive was found, which might have been due to faulty culture technique, or to the fact that the metabolic markers may persist longer than the OAB themselves. Because current methods to isolate non-sporulating OAB are laborious and not available in most veterinary clinical situations and because there is a need to evaluate the importance of non-sporulating OAB in veterinary medicine, GLC seems to be a useful alternative to culture techniques for detecting the presence of OAB in infectious processes in animals.
D000038:Abscess / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D001421:Bacteria, Anaerobic / Q000302:isolation & purification / Q000378:metabolism*; D001439:Bacteroides / Q000302:isolation & purification / Q000378:metabolism; D002849:Chromatography, Gas; D005232:Fatty Acids, Volatile / Q000032:analysis*; D005673:Fusobacterium / Q000302:isolation & purification / Q000378:metabolism; D013492:Suppuration / Q000382:microbiology; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D005232:Fatty Acids, Volatile