The rapid induction of liver cell death in rats fed a choline-deficient methionine-low diet.
This study was undertaken to test the hypothesis that the basis for the cell proliferation seen in the livers of rats fed a choline-deficient methionine-low (CMD) diet is regeneration following hepatocyte cell death and necrosis. Exposure of rats to a CMD diet for 2 weeks was found to induce liver cell loss and necrosis as monitored by three different approaches: 1) histologic examination, 2) serum sorbitol dehydrogenase assay, and 3) measurement of the total radioactivity in liver DNA prelabeled during a prior period of regeneration. These observations suggest that the basis for liver cell proliferation in rats fed a CMD diet probably resides in the cell loss and necrosis induced in the liver by the deficient diet.
D000818:Animals; D002455:Cell Division; D002470:Cell Survival; D002796:Choline Deficiency / Q000473:pathology*; D004247:DNA / Q000032:analysis; D007064:L-Iditol 2-Dehydrogenase / Q000097:blood; D008099:Liver / Q000648:ultrastructure*; D008115:Liver Regeneration; D008297:Male; D008715:Methionine / Q000172:deficiency*; D051381:Rats; D011916:Rats, Inbred F344
D004247:DNA; D008715:Methionine; D007064:L-Iditol 2-Dehydrogenase