Enhanced isolation of Campylobacter jejuni by cold enrichment in Campy-thio broth.
Isolation of Campylobacter jejuni from human feces by direct inoculation to Campy-BAP (Scott Laboratories, Inc., Fiskeville, R.I.) was compared with isolation after overnight enrichment at 4 degrees C in Campy-thio broth followed by subculture to Campy-BAP. Of 54 positive specimens, 19 were positive only after enrichment, and 5 were positive only on the direct plate. Among 36 positive patients, 10 were detected by enrichment only and 2 by direct plating only. Laboratories using Campy-BAP should include cold enrichment in Campy-thio broth for optimum recovery of C. jejuni.
D002168:Campylobacter fetus / Q000302:isolation & purification*; D003080:Cold Temperature; D003470:Culture Media; D005243:Feces / Q000382:microbiology; D006801:Humans; D012996:Solutions; D013048:Specimen Handling / Q000379:methods*
D003470:Culture Media; D012996:Solutions