Trichothecene-induced hemolysis. I. The hemolytic activity of T-2 toxin.
In the present investigation, the hemolytic properties of T-2 toxin were examined. Tests with different concentrations of T-2 toxin showed that essentially complete hemolysis of rat erythrocytes commenced after a lag period, the length of which depended on the concentration of toxin. Comparison of the characteristics of hemolysis caused by T-2 toxin, saponins, H2O2, and polyoxyethylene surfactants showed great similarity between T-2 toxin and the latter two which proceed by a free radical mechanism. The same mechanism was suggested for hemolysis caused by T-2 toxin on the basis of the additional following observations: (1) darkness inhibited hemolysis; (2) specific free radical scavengers, i.e., vitamin E, mannitol, and histidine, inhibited hemolysis caused by T-2 toxin.
D000818:Animals; D003624:Darkness; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005609:Free Radicals; D006461:Hemolysis / Q000187:drug effects*; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D051381:Rats; D012717:Sesquiterpenes / Q000633:toxicity*; D013605:T-2 Toxin / Q000633:toxicity*; D013997:Time Factors; D014255:Trichothecenes / Q000633:toxicity*
D005609:Free Radicals; D008054:Lipid Peroxides; D012717:Sesquiterpenes; D014255:Trichothecenes; D013605:T-2 Toxin