The measurement of lipid peroxidation products (lipofuscin) in individual cultivated human glial cells.
The natural fluorescence (autofluorescence) of the age pigment lipofuscin has been quantitated and related to the dry mass and DNA content (after Feulgen staining) of individual cultivated human glial cells which were density-dependent growth-inhibited for 3 and 18 months, subcultivated and kept in log growth phase for a period of up to 3 weeks. Stereological measurements at the ultrastructural level were performed in parallel to determine the volume density of secondary lysosomes of the residual body type (containing lipofuscin). The autofluorescence/dry mass/cell and the volume density of residual bodies showed a positive correlation. Cells in S-phase following subcultivation demonstrated similar autofluorescence values to cells in G1-phase. These results demonstrate that (1) multiparametric measurements of this type can be utilized to correlate many parameters which may be indicative of differing cell functions in individual cells, (2) there is a positive correlation between the ultrastructural and the autofluorescence techniques to measure lipofuscin, and (3) despite the limited data, even high cellular lipofuscin content does not appear to be detrimental to the ability of cells surviving subcultivation to re-enter the cell cycle.
D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D006801:Humans; D008054:Lipid Peroxides / Q000378:metabolism*; D008062:Lipofuscin / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009457:Neuroglia / Q000378:metabolism*; D010860:Pigments, Biological / Q000378:metabolism*
D008054:Lipid Peroxides; D008062:Lipofuscin; D010860:Pigments, Biological