title: Interaction between alkaline phosphatase and ascorbic acid by fluorescence and absorption studies.
abstract: Very low amounts of ascorbic acid modify alkaline phosphatase fluorescence, absorption and enzymatic activity. A strong quenching of enzyme, tryptophan and tyrosine emission together with evident alterations of the protein absorption characteristics are observed. The catalytic activity inhibition probably reflects a perturbation of the active site environment due to the interaction of ascorbic acid with enzyme aminoacyl residues.
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D002417:Cattle; D007668:Kidney / Q000201:enzymology; D013050:Spectrometry, Fluorescence
chemical_list: D000469:Alkaline Phosphatase; D001205:Ascorbic Acid