title: Mutual interactions in the transport of taurine, hypotaurine, and GABA in brain slices.
abstract: The mutual interactions and the effects of GABA on the saturable transport components of taurine and hypotaurine were investigated with mouse brain slices. The low-affinity taurine transport was competitively inhibited by both hypotaurine and GABA. Hypotaurine did not alter the kinetic parameters of high-affinity taurine uptake, whereas there occurred some stimulation with GABA, possibly by heteroexchange. Taurine had no significant effects on high-affinity hypotaurine uptake, whereas the low-affinity component was reduced by both taurine and GABA, GABA strongly interfered with the high-affinity hypotaurine uptake, being the preferred substrate in simultaneous uptake experiments. The results confirm that taurine, hypotaurine, and GABA are transported into brain slices by only one two-component system with affinities highest for GABA and lowest for taurine.
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D008297:Male; D051379:Mice; D013654:Taurine / Q000031:analogs & derivatives* / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D013654:Taurine; D005680:gamma-Aminobutyric Acid; C003949:hypotaurine