Binding of taurine to brain synaptic membranes.
An account is given of our endeavours to demonstrate sodium-independent and -dependent taurine binding to brain synaptic membranes. The total and specific sodium-dependent binding and the total sodium-independent binding were fairly easily amenable to characterization, but the disclosure of specific sodium-independent binding succeeded only after repeated freezing and thawing, thorough buffer washes and two Triton X-100 treatments of the membranes. The sodium-independent binding capacity for taurine was rather small and only one type of relatively low-affinity binding site was revealed.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D005260:Female; D005615:Freezing; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000502:physiology; D013045:Species Specificity; D013570:Synaptic Membranes / Q000378:metabolism*; D013654:Taurine / Q000378:metabolism*; D013696:Temperature
D013654:Taurine; D011092:Polyethylene Glycols; D017830:Octoxynol; D012964:Sodium; D002118:Calcium