Perfusion of the cervical spinal cord in situ of adult rats using a perfluorocarbon emulsion.
A method was developed for the perfusion of the cervical spinal cord of adult rats through the supplying arteries. A medium containing perfluorotributylamine as an oxygen carrier was used. The electrophysiological responsiveness was continuously monitored; pO2, pCO2, pH, Na+ and K+ were intermittently measured in the medium. The perfused cord maintained its responsiveness for more than 5 h. No changes in its structure became apparent in electron micrographs.
D000818:Animals; D001802:Blood Substitutes; D004655:Emulsions; D005466:Fluorocarbons; D006863:Hydrogen-Ion Concentration; D008297:Male; D008854:Microscopy, Electron; D009046:Motor Neurons / Q000502:physiology / Q000648:ultrastructure; D010100:Oxygen; D010313:Partial Pressure; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000502:physiology* / Q000648:ultrastructure
D001802:Blood Substitutes; D004655:Emulsions; D005466:Fluorocarbons; C008807:perfluorotributylamine; D010100:Oxygen