title: Acetonitrile-hydrochloric acid hydrolysis of gangliosides for high performance liquid chromatographic analysis of their long chain bases.
abstract: An improved method for the hydrolysis of long chain bases from gangliosides with aqueous acetonitrile-HCl is described. The long chain bases released from brain gangliosides were derivatized with biphenylcarbonylchloride and resolved by high performance liquid chromatography on a C18 reversed-phase column. Components of individual peaks were identified by gas-liquid chromatography-mass spectrometry as their trimethylsilyl derivatives. The acetonitrile-HCL hydrolysis procedure resulted in no formation of O-methyl ethers of long chain bases and a significant decrease in the level of secondary products.
mesh_terms: D000097:Acetonitriles; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005732:Gangliosides / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D006851:Hydrochloric Acid; D006868:Hydrolysis
chemical_list: D000097:Acetonitriles; D005732:Gangliosides; D006851:Hydrochloric Acid; C032159:acetonitrile