Purification and electron microscopy of a large plasmid of Rhizobium meliloti 41.
A large plasmid DNA molecule was purified from Rhizobium meliloti 41 by CsCl-ethidium bromide density gradient centrifugation. Electron microscopic and agarose gel electrophoretic data suggest that addition of alkali effectively removes the chromosomal DNA, the plasmid DNA can be precipitated from the cleared lysate and no gradient centrifugation is needed for plasmid purification.
D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D008854:Microscopy, Electron; D008970:Molecular Weight; D010957:Plasmids; D012231:Rhizobium / Q000235:genetics*
D004269:DNA, Bacterial