Nucleosome phasing in Tetrahymena macronuclei.
Core-protected DNA can drive only 60% of the Tetrahymena thermophila macronuclear genome into duplexes in hybridization experiments. This core-protected DNA therefore contains only a subset of the genome complexity. We interpret this to mean that a large fraction, if not all, of the genome is phased with respect to nucleosome placement. Among the sequences present in total DNA and absent from core-protected DNA are most of the sequences containing N6-methyladenine (MeAde) residues, consistent with our previous demonstration that most of these residues lie in linker DNA. We show that these results are not due to artifacts resulting from the small size of the DNA driver, nor are they due to any sequence preferences exhibited by staphylococcal (staph) nuclease. This is the first evidence that nucleosome phasing may be a bulk genome characteristic.
D000225:Adenine / Q000031:analogs & derivatives / Q000032:analysis; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000032:analysis; D008836:Micrococcal Nuclease / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D009707:Nucleosomes / Q000032:analysis*; D011189:Potassium Chloride / Q000494:pharmacology; D013768:Tetrahymena / Q000235:genetics / Q000648:ultrastructure*
D000225:Adenine; D004247:DNA; D008836:Micrococcal Nuclease; D009707:Nucleosomes; D011189:Potassium Chloride; C000730567:6-methyladenine