Selective culture of rat CNS neurons in a synthetic medium.
A Chemically Defined Medium is described which facilitates the survival of neurons in primary cultures of rat cerebral hemispheres. More than 90% of all cells were identified as neurons using neurofilament as a marker in an immunocytochemical assay. In contrast, serum-supplemented medium, by stimulating nonneuronal cell proliferation, hinders the survival of neurons in culture.
D000818:Animals; D001921:Brain; D002478:Cells, Cultured; D003470:Culture Media; D004622:Embryo, Mammalian; D009457:Neuroglia; D009474:Neurons; D051381:Rats
D003470:Culture Media