title: [Electron microscopic and morphometric studies of the main kidney segment of male and female rats following castration and testosterone substitution].
abstract: The effect of testosterone on the 3 segments of the renal proximal tubule (S1, S2, S3) of male and female rats was studied by electronmicroscopic and morphometric methods. Only light, granulated and dark lysosomes as well as microbodies (peroxisomes) and dictyosomes (Golgi zones) were investigated. After castration the area density of light lysosomes in the S1 segment increases in males whereas it decreases in females; therefore the sex different pattern of light lysosomes, that is to be seen in normal animals, is reversed. The absolute size and number of light giant lysosomes is also elevated in castrated males in comparison to normal animals as well as to animals substituted by testosterone. - Dark lysosomes of the S1 segments are more numerous in castrated females and less numerous in castrated males than in normal animals. - The distinct sex difference in dark lysosomes of the S2 segment which is demonstrable in normal animals disappears after castration the area density of dark lysosomes increasing in castrated females and decreasing in castrated males. The three species of lysosomes in the S1 segments show no longer a sex difference after substitution with testosterone: substituted males develop the same pattern as normal animals and substituted females are almost comparable with normal males. However, the sex difference in dark lysosomes of the S2 segment is more pronounced after testosterone treatment. - The characteristic pattern of light lysosomes in the S1 and S2 segments as well as the change of the sex different lysosomal pattern after castration and substitution with testosterone, respectively - especially in S1 - seem to be caused by testosterone which results in an inhibition of resorption. Only after castration a sex difference appears in dark lysosomes of the S3 segment (males show more dark lysosomes than females). This sex difference is reversed by testosterone treatment. There are more numerous lysosomes with an non-homogeneous matrix in both sexes after castration which are seldom to be seen in normal and substituted animals. The area density of microbodies shows sex differences in all 3 segments of normal animals. While no significant changes in S1 and S2 are to be seen after castration and substitution, there is a pronounced decrease of the area density of microbodies in S3 of males after castration, so that no sex differences are then available.(ABSTRACT TRUNCATED AT 400 WORDS)
mesh_terms: D000818:Animals; D002369:Castration / Q000662:veterinary*; D005260:Female; D006056:Golgi Apparatus / Q000648:ultrastructure; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000648:ultrastructure*; D008247:Lysosomes / Q000648:ultrastructure; D008297:Male; D008830:Microbodies / Q000648:ultrastructure; D009940:Organoids / Q000187:drug effects / Q000648:ultrastructure*; D051381:Rats / Q000033:anatomy & histology*; D011919:Rats, Inbred Strains; D012737:Sex Factors; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D013739:Testosterone
vernacular_title: Elektronenmikroskopisch-morphometrische Untersuchungen am Nierenhauptstück männlicher und weiblicher Ratten nach Kastration und Testosteronsubstitution.