Inhibition of programmed cell death in mouse embryonic palate in vitro by cortisol and phenytoin: receptor involvement and requirement of protein synthesis.
In an in vitro model cortisol and phenytoin inhibit the precisely timed process of palatal development, the lysosomally mediated cell death of the medial edge palatal epithelium. This inhibition of programmed cell death of the palatal midline epithelium by each drug is virtually completely blocked by the antiglucocorticoid, cortexolone, whose blocking action results from competitive binding of the glucocorticoid receptor site. The inhibition produced by each of these drugs is prevented by the protein synthesis blocker, cycloheximide. Thus, blockade of programmed cell death by each of these drugs involves the glucocorticoid receptor site and requires protein synthesis.
D000818:Animals; D001667:Binding, Competitive; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D003350:Cortodoxone / Q000494:pharmacology; D005260:Female; D006854:Hydrocortisone / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010159:Palate / Q000196:embryology*; D010672:Phenytoin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011965:Receptors, Glucocorticoid / Q000187:drug effects* / Q000378:metabolism; D011987:Receptors, Steroid / Q000187:drug effects*
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D010672:Phenytoin; D003350:Cortodoxone; D006854:Hydrocortisone