Microscale purification of proteins by line immunoelectrophoresis: application of the technique in protein biogenesis studies.
A small-scale version of line immunoelectrophoresis in combination with immunoprecipitate excision is described as a rapid and convenient technique to purify proteins on a micro scale in biogenesis studies. In the purification and to result in a higher state of purity than an isolation procedure using protein A-Sepharose. Since the method furthermore allows a simultaneous purification of several different protein antigens from the same sample, it may be of interest as an alternative method to other procedures in the purification of proteins on a micro scale.
D000818:Animals; D007122:Immunoelectrophoresis / Q000379:methods; D007421:Intestine, Small / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification*; D013552:Swine
D011506:Proteins