Electron-capture determination of metoclopramide in biological fluids using fused silica capillary columns. Application to placental transport studies in sheep and humans.
An electron-capture gas-liquid chromatographic assay for metoclopramide using cross-linked fused silica capillary columns which provides improved selectivity and sensitivity is reported. A 25 m X 0.31 mm fused silica capillary column was used for all analyses. Linearity was observed in the range of 4--40 ng of metoclopramide base per 0.25--0.5 ml of plasma. This represents from ca. 0.9--9.0 pg at the detector employing a split ratio of 30:1 and an injection volume of 2 microliters. Applicability of the method is demonstrated by the analysis of human and sheep plasma (maternal, fetal and neonatal) from metoclopramide placental transfer studies.
D000328:Adult; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D002849:Chromatography, Gas / Q000379:methods; D005260:Female; D005312:Fetal Blood / Q000032:analysis; D005333:Fetus / Q000378:metabolism; D006207:Half-Life; D006801:Humans; D007231:Infant, Newborn; D008431:Maternal-Fetal Exchange; D008787:Metoclopramide / Q000097:blood*; D011247:Pregnancy; D012756:Sheep
D008787:Metoclopramide