title: Incomplete glycosylation of Asn 563 in mouse immunoglobulin M.
abstract: Mouse immunoglobulin IgM was prepared from MOPC 104E ascites fluid and [3H]-mannose labeled tumor cells. The purified protein was used to prepare Fc fragments which were cleaved by cyanogen bromide. Gel filtration allows complete separation of the C-terminal glycosylation site. Amino acid and carbohydrate analyses show that Asn 563 of murine IgM is glycosylated only about 44% of the time.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001216:Asparagine / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis; D003488:Cyanogen Bromide; D007141:Immunoglobulin Fc Fragments / Q000032:analysis; D007075:Immunoglobulin M / Q000378:metabolism*; D051379:Mice
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D007141:Immunoglobulin Fc Fragments; D007075:Immunoglobulin M; D001216:Asparagine; D003488:Cyanogen Bromide