Sensitive and specific post-column fluorimetric determination of diethylstilboestrol residues in extracts of urine and animal tissues at the 1-ppb level.
A rapid method for the selective detection and quantitation of diethylstilboestrol (DES) residues at the 1 ppb level in extracts of urine and animal tissues is described. After selective extraction of the oestrogens, DES is analysed by high-performance liquid chromatography using an in-line specific photochemical reactor followed by oxidation to highly fluorescent products. The reaction products of DES were investigated by thin-layer chromatography (TLC). The specificity of the proposed method was compared with that of a reference TLC method on different extracts of animal origin.
D000273:Adipose Tissue / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D004054:Diethylstilbestrol / Q000032:analysis* / Q000652:urine; D008460:Meat / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods; D014020:Tissue Extracts / Q000032:analysis*
D014020:Tissue Extracts; D004054:Diethylstilbestrol