Antigenic properties of estriol 3-glucuronide-[C-6]-bovine serum albumin conjugates having oxime bridges.
The preparation and antigenic properties of estriol 3-glucuronide-bovine serum albumin (BSA) conjugate in which the hapten is linked to the carrier protein through an (O-3-carboxypropylcarbamoylmethyl)oxime bridge at the C-6 position on the steroid nucleus, has been described. 16,17-Di-O-acetyl-6-oxoestriol 6-(O-carboxymethyl)oxime 3-glucuronide acetate-methyl ester was condensed with gamma-amino-n-butyric acid by the mixed anhydride method. Subsequent coupling with BSA followed by removal of the protecting groups with alkali gave the desired hapten-BSA conjugate. The antisera elicited in the rabbit with the conjugate were highly specific to estriol 3-glucuronide, discriminating from other 3-substituted compounds. The specificity of antisera improved by elongation of the 6-(O-carboxymethyl)oxime bridge has been discussed.
D000818:Animals; D000918:Antibody Specificity; D000941:Antigens; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D003429:Cross Reactions; D004964:Estriol / Q000031:analogs & derivatives* / Q000276:immunology; D010091:Oximes; D012710:Serum Albumin, Bovine / Q000276:immunology
D000941:Antigens; D010091:Oximes; C022810:estriol 3-glucuronide; D012710:Serum Albumin, Bovine; D004964:Estriol