Blood-brain barrier transport of L-pipecolic acid in various rat brain regions.
Blood-brain barrier transport of L-[1-14C]pipecolic acid was studied in the ray by single intracarotid injection using 3H2O as a diffusible internal standard. Brain uptake index (BUI) for L-[14C]pipecolic acid (0.036 mM) was found to be 18.1, 10.5, and 12.6 for the cerebral cortex, brain stem, and cerebellum, respectively which was substantially higher than that reported for its analog L-proline in the whole brain. Influx of L-pipecolic acid into the brain was concentration dependent and differed significantly between the cerebral cortex and the brain stem, and between the cerebral cortex and the cerebellum, but not between the brain stem and the cerebellum. Kinetic study of L-pipecolic acid influx revealed a low- and a high-capacity uptake mechanisms. The low-capacity saturable component has Km values ranging from 38 to 73 microM, and Vmax values ranging from 10 to 13 nmol/g/min for the three brain regions. The nonsaturable component has a Km of 4 mM, a Vmax of 200 nmol/g/min and similar diffusion constant (Kd) (0.03 to 0.06 mlg-1 min-1) for all three brain regions. A possible role of the two-component brain uptake mechanism in the regulation of the neuronal function of L-pipecolic acid was suggested.
D000818:Animals; D001692:Biological Transport; D001812:Blood-Brain Barrier; D007700:Kinetics; D008297:Male; D010875:Pipecolic Acids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D010875:Pipecolic Acids