An enzymatic lysis procedure for the assay of enzymes in Thiobacillus A2.
A micromethod is described for the production of lysed preparations of Thiobacillus A2 following treatment with lysozyme and EDTA. These may be used for the assay of intra-cellular enzymes including rhodanese, hexokinase, glucose 6-phosphate dehydrogenase and phosphoglucoseisomerase. The procedure is useful for assaying enzymes in samples too small to be treated by conventional mechanical methods, but gives comparable recoveries of enzyme activities.
D007700:Kinetics; D008832:Microchemistry; D009113:Muramidase; D013855:Thiobacillus / Q000201:enzymology*; D013884:Thiosulfate Sulfurtransferase / Q000032:analysis
D013884:Thiosulfate Sulfurtransferase; D009113:Muramidase