Effects of 16,16-dimethyl prostaglandin E2 on alkaline secretion in isolated canine gastric mucosa.
An isolated fundic mucosal preparation of dog stomach which is capable of exhibiting an alkaline secretion is described. A stable secretion was established 40 min to 1 hr after the mucosa was pretreated with the H2-antagonist cimetidine to block spontaneous acid output. Alkaline secretion decreased when Ca2+ was removed from the nutrient solution. This secretion was stimulated by dibutyryl cyclic GMP, but was not altered by acetylcholine, carbachol, or 16,16-dimethyl PGE2. Alkaline secretion from a similar antral mucosal preparation was stimulated by 16,16-dimethyl PGE2. We conclude that the 16,16-dimethyl PGE2-stimulated bicarbonate secretion previously demonstrated in in vivo canine fundic mucosa is not the result of a direct effect of PG on gastric mucosal cells and that an intact blood circulation or cholinergic innervation is required for this action to occur.
D015064:16,16-Dimethylprostaglandin E2 / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000502:physiology; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology; D004285:Dogs; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology*; D013997:Time Factors
D011459:Prostaglandins E, Synthetic; D003995:Dibutyryl Cyclic GMP; D015064:16,16-Dimethylprostaglandin E2; D002118:Calcium