[Radioimmunological analysis of the neuraminidase of influenza virus subtype N2].
Competitive radioimmunoassay in the homologous system using 125I-labeled neuraminidase (NA) of A/Japan/305/57 virus and antibodies to it showed that influenza viruses NA of subtypes H2N2 (A/Singapore/1/57 and A/Tokyo/3/67) and H3N2 (A/Aichi/2/68, A/England/42/72, A/Port Chalmers/1/73, and A/Texas/1/77) had undergone clear antigenic change despite the presence of Japan strain NA determinant. The use of a heterologous system (125I-NA of the Japan strain--antibodies to NA of England/42/72 strain) for the study of the intratype determinant showed the presence of a common determinant in all the strains under study, this determinant being represented dissimilarly, however. Neuraminidase of the Texas strain has a determinant differing from the intratype one, common for the Japan and England viruses.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D007114:Immunization; D009980:Influenza A virus / Q000201:enzymology* / Q000276:immunology; D009439:Neuraminidase / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods
D000954:Antigens, Surface; D000956:Antigens, Viral; D009439:Neuraminidase
Radioimmunologicheskiĭ analiz neĭraminidazy virusa grippa podtipa N2.