Spectrophotometric quantitation of silver grains eluted from autoradiograms.
Autoradiograms or fluorograms can be quantified by eluting the silver grains from the developed film with 1 M NaOH and measuring the absorbance of the eluate in a conventional spectrometer. In contrast to direct scanning of the film, the present method is rapid, requires only simple equipment, and permits accurate quantitation of even heavily exposed bands.
D001345:Autoradiography / Q000379:methods*; D005453:Fluorescence; D012834:Silver; D013053:Spectrophotometry / Q000379:methods*
D012834:Silver