title: An effective method of DNA isolation from the mature leaves of Gossypium species that contain large amounts of phenolic terpenoids and tannins.
abstract: Purified and unstained nuclei were isolated from the leaves of several Gossypium species (diploid and tetraploid) by means of a citrate buffer (pH 5.0), Triton X-100 (5%), and a reducing sugar (1M glucose). DNA, previously unobtainable, was then extracted from the nuclei by conventional means. Comparisons of final DNA yield were made between three methods of purification: namely, the standardized ribonuclease procedure, hydroxyapatite chromatography and equilibrium density centrifugation in cesium chloride. The latter method produce the lowest, yet purest, yield of DNA for renaturation studies in Gossypium.
mesh_terms: D002467:Cell Nucleus / Q000032:analysis; D004247:DNA / Q000302:isolation & purification*; D005947:Glucose; D003368:Gossypium / Q000032:analysis* / Q000235:genetics; D013634:Tannins; D013696:Temperature; D013729:Terpenes
chemical_list: D013634:Tannins; D013729:Terpenes; D004247:DNA; D005947:Glucose