The lipopolysaccharide of a chloridazon-degrading bacterium.
Lipopolysaccharide of a chloridazon-degrading bacterium was obtained by a two-stage extraction procedure with phenol/EDTA in a yield of 0.3% of dried bacteria. The carbohydrate moiety consisted of heptose, 3-deoxyoctulosonic acid and D-glucose in a molar ratio of 1:2:2 X 3. Lipid A was composed of 1 mol 2,3-diamino-2,3-dideoxy-D-glucose, 2 mol amide-bound and 2.6 mol ester-bound fatty acids/mol. Amide-bound fatty acids were 3-hydroxydodecanoic acid and 3-hydroxyhexadecanoic acid; dodecanoic acid and R-(-)-3-hydroxydodec-5-cis-enoic acid were found to be present in ester linkage. Under conditions of acidic hydrolysis, the latter was converted into the cis and trans isomers of 5-hexyltetrahydrofuran-2-acetic acid. Dodecanoic acid was demonstrated to be linked with the hydroxy groups of the amide-bound fatty acids. The taxonomic significance of these results, especially the demonstration of 2,3-diamino-2, 3-dideoxy-D-glucose, is discussed.
D001419:Bacteria / Q000145:classification / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D003124:Colorimetry; D004590:Electrophoresis, Paper; D005227:Fatty Acids / Q000032:analysis; D005944:Glucosamine / Q000031:analogs & derivatives / Q000302:isolation & purification; D006540:Herbicides / Q000378:metabolism*; D006880:Hydroxy Acids / Q000302:isolation & purification; D008050:Lipid A / Q000032:analysis; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D008745:Methylation; D011724:Pyridazines / Q000378:metabolism*; D012988:Soil Microbiology
D005227:Fatty Acids; D006540:Herbicides; D006880:Hydroxy Acids; D008050:Lipid A; D008070:Lipopolysaccharides; D011724:Pyridazines; C005586:pyrazon; C040138:2,3-diamino-2,3-dideoxyglucose; C040139:methyl-3-hydroxydodec-5-enoic acid; D005944:Glucosamine