[3H]diazepam binding sites on chick neurons in primary culture.
High affinity [3H]diazepam binding sites were identified on neurons prepared from the hemispheres of 8-day-old chick embryos and grown in serum-containing or serum-free medium. Clonazepam (IC50 = 3 nM) was more potent than Ro 5-4864 (IC50 greater than 1000 nM) in displacing [3H]diazepam binding. GABA and pentobarbital, in the presence of chloride ions were able to stimulate [3H]diazepam binding synergistically. These interactions were found to be comparable to those observed in mammalian brain.
D000818:Animals; D001570:Benzodiazepinones / Q000378:metabolism; D001665:Binding Sites / Q000187:drug effects; D001667:Binding, Competitive; D002478:Cells, Cultured; D002642:Chick Embryo; D002998:Clonazepam / Q000378:metabolism; D003975:Diazepam / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D010424:Pentobarbital / Q000494:pharmacology; D013687:Telencephalon / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D001570:Benzodiazepinones; C028513:4'-chlorodiazepam; D005680:gamma-Aminobutyric Acid; D002998:Clonazepam; D010424:Pentobarbital; D003975:Diazepam