Effects of oviducal cells on the maintenance of motility and fertilizing capacity of fowl spermatozoa stored in a diffusion chamber.
Oviducal cells were cultured on the inner or outer side of the membrane filter of a diffusion chamber, and attempts were made to maintain fowl spermatozoa at 41 C in vitro. When spermatozoa were stored along with cultured cells in the diffusion chamber, the high fertilizing capacity was retained for at least 3 days, regardless of whether the spermatozoa stored in the diffusion chamber were in direct contact with the cultured cells. In contrast, spermatozoa stored in the cell-free diffusion chamber or in a test tube showed a relatively low or no fertilizing capacity, respectively.
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D002645:Chickens; D004058:Diffusion; D005260:Female; D005306:Fertilization; D008297:Male; D010057:Oviducts / Q000502:physiology*; D012662:Semen Preservation / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*