Leishmanial excreted factors (EFs): purification by affinity chromatography.
Leishmania species grown in culture excrete a polyanionic, carbohydrate-rich factor (EF) which binds to antibodies produced in rabbits against the parent Leishmania species. EF, previously purified by physical and chemical methods, was purified by affinity chromatography on a Ricinus lectin column. The purified samples were characterised and analysed. The results show a notable proportion of galactose in EF and clarify the reasons for its polyanionic properties. Heterogenicity of EF is demonstrated and discussed.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002846:Chromatography, Affinity; D005690:Galactose / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification*; D007891:Leishmania / Q000032:analysis*; D008970:Molecular Weight; D013455:Sulfur / Q000032:analysis
D000596:Amino Acids; D002241:Carbohydrates; D006023:Glycoproteins; D013455:Sulfur; D005690:Galactose