The identity of conformational states of reconstituted and native histone octamers.
Histone octamers were reconstituted from the following preparations: (a) natural histone H3-H4 tetramer and histone H2A-H2B dimer, either selectively extracted from chromatin with solutions chloride or prepared by dissociation of the natural octamer; (b) acid-denatured core histones, either an unfractionated mixture or individually purified proteins. Complexes assembled from these histones elute from exclusion chromatography columns with octamer size as verified by cross-linking with dimethylsuberimidate. The reconstituted octamers all crystallize in the same form of helical tubes as the natural octamer.
D000818:Animals; D001704:Biopolymers; D002645:Chickens; D002845:Chromatography / Q000379:methods; D003432:Cross-Linking Reagents; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000302:isolation & purification; D008854:Microscopy, Electron; D011487:Protein Conformation; D011489:Protein Denaturation; D014461:Ultracentrifugation
D001704:Biopolymers; D003432:Cross-Linking Reagents; D006657:Histones