title: Muscle protein turnover in cattle of differing genetic backgrounds as measured by urinary N tau-methylhistidine excretion.
abstract: N tau-methylhistidine (N tau MH) was used as an index for muscle protein degradation and this index was utilized to evaluate degradation rates in young growing cattle. Initially, two Charolais crossbred heifers, 12 months of age, were used to measure the recovery of radioactivity in the urine for a 120-hour period after intravenous injection of [14C]N tau MH. Of the radioactivity injected into the animals, 89.7% was recovered after 120 hours. With rate and amount of clearance as the criteria, the excretion of N tau MH in urine appears to be a valid index of muscle protein degradation in cattle. Eight steers of two genetic types were used to evaluate the effect of frame size on turnover rates of muscle proteins with N tau MH as an index. Large frame cattle (LG) excreted more N tau MH per day (P less than 0.03) throughout the trial. Total daily creatinine excretion was less for small frame (SM) cattle (P less than 0.02) showing an increase with time (P less than 0.01) in LG and SM cattle. N tau MH-to-creatinine ratios showed a decline with time. Fractional breakdown rates (FBR) and fractional synthesis rates (FSR) appeared to parallel each other with rates tending to decrease with age. No differences were observed between LG and SM cattle for FBR, FSR or fractional growth rate (FGR).
mesh_terms: D000818:Animals; D001835:Body Weight; D002250:Carbon Radioisotopes; D002417:Cattle / Q000235:genetics / Q000378:metabolism*; D003404:Creatinine / Q000652:urine; D003433:Crosses, Genetic; D005260:Female; D006639:Histidine / Q000031:analogs & derivatives*; D008297:Male; D008762:Methylhistidines / Q000652:urine*; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
chemical_list: D002250:Carbon Radioisotopes; D008762:Methylhistidines; D009124:Muscle Proteins; D006639:Histidine; D003404:Creatinine