A study of candidate rabies vaccines for a national reference preparation for the evaluation of antigenic potency.
An unconcentrated and a concentrated lot of rabies vaccine prepared in Syrian hamster kidney cell culture from the Vnukovo-32 strain have been examined for their suitability as national reference preparations for rabies vaccine. The antigenic potencies of the preparations were assessed by the NIH test using both fixed and street strains of rabies virus and by the method of antibody induction. The candidate vaccines were calibrated in comparative assays with the WHO 3rd International Reference Preparation of Rabies Vaccine (IRP3). The correlation between the level of virus-neutralizing antibody and the resistance of mice to intracerebral challenge with a fixed rabies virus strain was studied. The candidate vaccines were also examined by the thermal degradation test. It was found that both vaccines had appropriate antigenic potency and thermostability to be used as a national reference preparation of rabies vaccine.
D000818:Animals; D000917:Antibody Formation; D000956:Antigens, Viral / Q000592:standards*; D004355:Drug Stability; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011819:Rabies Vaccines / Q000592:standards*; D012015:Reference Standards; D014586:USSR
D000956:Antigens, Viral; D011819:Rabies Vaccines