Isolation and identification of dihydrocitrinone, a urinary metabolite of citrinin in rats.
Dihydrocitrinone, 3,4-dihydro-6,8-dihydroxy-3,4,5-trimethylisocoumarin-7-carboxylic acid, was isolated and identified as a urinary metabolite after oral administration of citrinin to rats. Male and female Osborne-Mendel rats received 30 mg citrinin/kg body weight by oral intubation. The metabolite dihydrocitrinone was present in urine collected at 0-2, 2-4, 4-6, 6-8, and 8-24 h after treatment. Only unchanged citrinin was found in blood collected 24 h after administration of the compound. The metabolite had a blue fluorescence and the same Rf on thin-layer chromatography, the same retention time on reverse-phase high-pressure liquid chromatography, and the same mass spectrum as an authentic sample of dihydrocitrinone.
D000818:Animals; D001578:Benzopyrans / Q000378:metabolism* / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D002953:Citrinin / Q000031:analogs & derivatives / Q000097:blood / Q000378:metabolism* / Q000652:urine*; D005260:Female; D008297:Male; D013058:Mass Spectrometry; D051381:Rats
D001578:Benzopyrans; D002953:Citrinin; C015142:dihydrocitrinone