Influence of dimethylsulphoxide on the functional state of isolated rat hepatocytes.
The effect of dimethylsulphoxide upon respiration, glycolysis and gluconeogenesis in isolated rat hepatocytes was studied. The results show that the glycolysis pathway is strongly inhibited by the action of dimethylsulphoxide in the hepatocytes, with increased glucose uptake and reduced net flux. However, the alterations in respiration and the gluconeogenesis pathway are less pronounced, the only significant change being an increase in respiratory activity during the first 10 min of incubation with dimethylsulphoxide.
D000818:Animals; D002478:Cells, Cultured; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005943:Gluconeogenesis / Q000187:drug effects; D006019:Glycolysis / Q000187:drug effects; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D004121:Dimethyl Sulfoxide