Laccase assay by means of high-performance liquid chromatography.
Spectrophotometric determination of laccase activity may be affected by the formation of quinoid chromophores arising from nonenzymatic oxidations interfering with enzymatic reactions. Km values for guaiacol obtained by spectrophotometric and HPLC methods confirm the above hypothesis. HPLC results are particularly useful for the assay of laccase activity on natural phenolic extracts.
D002851:Chromatography, High Pressure Liquid; D006139:Guaiacol; D042845:Laccase; D010088:Oxidoreductases / Q000032:analysis*; D010636:Phenols; D013053:Spectrophotometry
D010636:Phenols; D006139:Guaiacol; D010088:Oxidoreductases; D042845:Laccase