title: Low-energy Mg2+-induced temperature transitions in liver microsomes.
abstract: The differential scanning microcalorimetry and fluorescence methods, using probes ANS and pyrene, have been employed to study thermotropic behaviour of rat liver microsomes in the presence and absence of Mg2+. Addition of Mg2+ yields three partially reversible phase transitions at 18, 27 and 32 degrees C, respectively. A character of Mg2+-induced rearrangements in a membrane and their relation to a catalytic function of a cytochrome P-450-dependent enzymatic system is discussed.
mesh_terms: D000818:Animals; D002152:Calorimetry, Differential Scanning; D008274:Magnesium / Q000494:pharmacology*; D008862:Microsomes, Liver / Q000187:drug effects*; D051381:Rats; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D008274:Magnesium