Subcellular localization of human heart atypical creatine kinase.
Human heart subcellular fractions were obtained using method A described by Scholte et al [7,8]. It was found that 3.5 +/- 2.5%, 3.4 +/- 1.9%, 8.5 +/- 2.9%, 2.5 +/- 1.3%, and 88 +/- 21% of the total creatine kinase activity was associated with the myofibrillar, nuclear, mitochondrial, microsomal and particle-free soluble fractions respectively. Using DEAE-sephacel chromatography it was determined that the atypical creatine kinase (CK-Z) accounted for approximately 90% of the creatine kinase activity associated with the mitochondrial fraction.
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D003402:Creatine Kinase / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology; D006801:Humans; D007527:Isoenzymes; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008929:Mitochondria, Heart / Q000201:enzymology; D009206:Myocardium / Q000648:ultrastructure*; D009210:Myofibrils / Q000201:enzymology; D051381:Rats
D007527:Isoenzymes; D003402:Creatine Kinase