Isolation of bovine polymorphonuclear leukocytes by density gradient centrifugation.
A method for the isolation of an enriched population (greater than 95%) of bovine polymorphonuclear leukocytes (PMNs) was developed using density gradient centrifugation. Leukocytes were isolated from peripheral blood by centrifugation in a density gradient medium (Percoll) of specific gravity 1.092. Viability was greater than or equal to 95% and the isolated PMNs were functional in migration inhibition and chemiluminescence assays. This has proved to be a simple effective method for obtaining bovine PMNs and yields cell populations that can be utilized for a variety of measures of PMN function.
D000818:Animals; D002417:Cattle; D002464:Cell Migration Inhibition; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002499:Centrifugation, Density Gradient; D009504:Neutrophils / Q000276:immunology; D010587:Phagocytosis