Protuberic acid-hydrolytic enzyme in Kobayasia nipponica and characterization of the hydrolytic products.
When the cold water-extract of Kobayasia nipponica containing a glycuronan, protuberic acid (PA), was allowed to stand at room temperature, PA was hydrolyzed. The optimum conditions for this PA-hydrolysis were 37 degrees C and pH 4-5 in 0.1 M acetate buffer. Characterization of the hydrolytic products was performed by chemical analysis, and by 1H- and 13C-NMR spectroscopy. They were identified as D-GlcUA and O-(alpha-L-IdUAp)-(1-4)-D-GlcUA. These results suggest that PA-hydrolytic enzyme(s) include at least endo-beta-D-glucuronidase.
D001487:Basidiomycota / Q000201:enzymology*; D002240:Carbohydrate Sequence; D005966:Glucuronidase / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000302:isolation & purification*
D011134:Polysaccharides; C040159:protuberic acid; D005966:Glucuronidase