Monosialoganglioside (GM1) treatment of ouabain-induced retinopathy in the rabbit.
The mammalian retina is markedly influenced by cardiac glycosides. When nanomolar concentrations of ouabain are intravitreously injected into the rabbit, the visually evoked response completely disappears within 90 min, while scotopic ERG recordings show a remarkably decreased amplitude of the principal waves. When 33 nmol/kg monosialoganglioside are injected intravenously 30 min before topical intoxication, this functional impairment is significantly reduced. The electroretinographic response shows a limited amplitude reduction, while the cortical potential never disappears completely. Histological observations of intoxicated retinas show that a degenerative process begins in photoreceptor outer segment 24 h after the intravitreal ouabain injection. Presently, this process involves both the outer and inner nuclear layers and, finally, the ganglion cell layer. Comparing the intoxicated treated and untreated retinas, no difference is found in the degenerative pattern of the two groups. Autoradiographic studies are also reported to correlate the protective effect of monoganglioside (GM1) on this toxic retinopathy with its preferential accumulation in different retinal tissues.
D000818:Animals; D004596:Electroretinography; D005074:Evoked Potentials, Visual; D005677:G(M1) Ganglioside / Q000008:administration & dosage / Q000627:therapeutic use*; D005732:Gangliosides / Q000627:therapeutic use*; D007275:Injections, Intravenous; D008297:Male; D010042:Ouabain / Q000633:toxicity*; D011817:Rabbits; D012162:Retinal Degeneration / Q000139:chemically induced; D012164:Retinal Diseases / Q000139:chemically induced / Q000188:drug therapy*; D013997:Time Factors
D005732:Gangliosides; D005677:G(M1) Ganglioside; D010042:Ouabain