Development and characterization of a homologous radioimmunoassay for deer mouse (Peromyscus maniculatus bairdii) prolactin.
A highly specific and sensitive homologous radioimmunoassay has been developed for the secreted form of prolactin from the deer mouse Peromyscus maniculatus bairdii. Peromyscus serum and pituitary homogenates displayed parallel dilution response curves, and no cross reaction was seen with either mouse prolactin, mouse growth hormone or rat prolactin. The assay was sensitive to 25 picograms per tube and the intra- and inter-assay coefficients of variation were 5 and 3.6%, respectively. In addition, we have demonstrated that Peromyscus prolactin does not show parallel displacement in a homologous radioimmunoassay utilized for measuring prolactin in the common laboratory mouse.
D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology; D005260:Female; D007774:Lactation; D008297:Male; D010542:Peromyscus / Q000097:blood / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000032:analysis; D011247:Pregnancy; D011388:Prolactin / Q000032:analysis* / Q000097:blood; D011863:Radioimmunoassay / Q000379:methods
D001971:Bromocriptine; D011388:Prolactin