title: Intracellular Na+ and Ca2+ in aortic smooth muscle cells after enzymatic isolation in spontaneously hypertensive rats.
abstract: In aortic smooth muscle cells from spontaneously hypertensive and normotensive rats, which were separated from the blood vessels by enzymatic digestion, intracellular Na+ and Ca2+ activities were measured. The determinations were made by ion-selective electrodes. In smooth muscle cells from spontaneously hypertensive rats intracellular Na+ and Ca2+ activities were elevated as compared to cells from normotensive rats (P less than 0.001 for Na+ and P less than 0.01 for Ca2+). Since in cultured aortic smooth muscle cells from spontaneously hypertensive rats intracellular Ca2+ is known to be not significantly increased, primarily humoral factors may account for the changes in intracellular Ca2+ reported.
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000378:metabolism; D001826:Body Fluids / Q000378:metabolism*; D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D002469:Cell Separation; D004566:Electrodes; D006973:Hypertension / Q000378:metabolism*; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000032:analysis / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D012964:Sodium; D002118:Calcium