title: Bovine lens aldehyde dehydrogenase. Purification and preliminary characterization.
abstract: Cytoplasmic aldehyde dehydrogenase from bovine lens was purified to apparent homogeneity by using ion-exchange and affinity chromatography. Sedimentation-equilibrium ultracentrifugation, gel-filtration chromatography and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis show that the enzyme is a dimer of Mr 114000, with subunits of Mr 57000. The enzyme does not dissociate into monomers in the presence of Ca2+ or Mg2+. The enzyme has a pI of 5.0, an activation energy of 35.1kJ/mmol and a pK value of 8.6 with acetaldehyde as substrate. The enzyme is a prolate ellipsoid with a Stokes radius of 4nm. Progesterone, deoxycorticosterone and chlorpropamide inhibited enzyme activity, and this inhibition may play a role in cataract formation in patients maintained on systemic corticosteroids and in tablet-dependent diabetics.
mesh_terms: D000444:Aldehyde Dehydrogenase; D000445:Aldehyde Oxidoreductases / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D002747:Chlorpropamide / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007908:Lens, Crystalline / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D013256:Steroids / Q000494:pharmacology; D014461:Ultracentrifugation
chemical_list: D000596:Amino Acids; D013256:Steroids; D000445:Aldehyde Oxidoreductases; D000444:Aldehyde Dehydrogenase; D008274:Magnesium; D002118:Calcium; D002747:Chlorpropamide