Effect of seminal plasma and calcium on the stability of the surface protein composition of ejaculated bull spermatozoa.
Ejaculated bull spermatozoa were incubated in different media to elucidate the effect of seminal plasma and calcium on the stability of sperm surface compositions. The spermatozoa were radioiodinated prior to or after the incubation and the labelled proteins from the incubation media and spermatozoa were analysed by SDS-polyacrylamide gel electrophoresis. Seminal plasma detached efficiently sperm surface components. Even with concentration of 2% considerable amounts of the 44K and 17K proteins were released into the medium. The 17K proteins of seminal plasma were also adsorbed by sperm surfaces during incubation. The release of the 27K protein seemed to be dependent on calcium.
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002417:Cattle; D004355:Drug Stability; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D012661:Semen / Q000502:physiology*; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism*
D008563:Membrane Lipids; D008565:Membrane Proteins; D002118:Calcium