Synthesis of ppTppp via phosphotriester intermediates.
The bifunctional and crystalline phosphorylating agent morpholino-0,0-bis[1-benzotriazolyl]phosphate has been used for the preparation of a 3',5'-bis-phosphotriester intermediate of thymidine. The latter has been converted into ppTppp by the following consecutive steps; removal of the benzotriazolyl group followed by the addition of phosphoric acid and removal of the 2-(4-nitrophenyl)-ethyl group followed by the addition of pyrophosphoric acid.
D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D013237:Stereoisomerism; D013936:Thymidine; D013942:Thymine Nucleotides / Q000138:chemical synthesis*
D007202:Indicators and Reagents; D013942:Thymine Nucleotides; C025437:thymidine-5'-tri-3'-diphosphate; D013936:Thymidine