An improved colony hybridization method with significantly increased sensitivity for detection of single genes.
By introducing a simple modification of existing methods, colony hybridization has been used to detect single copy genes coding for a heat-stable enterotoxin in wild-type strains isolated from patients with diarrhea. The modification described in this communication results in an approximately 100-fold increase in sensitivity, probably by increasing the total denatured plasmid DNA fixed to the paper.
D003001:Cloning, Molecular / Q000379:methods*; D006358:Hot Temperature; D009693:Nucleic Acid Hybridization; D013997:Time Factors