Preparation of a covalently linked adduct between plastocyanin and cytochrome f.
Plastocyanin can be covalently cross-linked to the monomeric cytochrome f from turnip by incubation in the presence of a water-soluble carbodiimide. The adduct between the two proteins has a molecular weight of approximately 43,000 suggesting a 1:1 stoichiometry between the two proteins of the adduct. This stoichiometry has been verified by spectral characterization of the adduct. The efficiency of the cross-linking reaction is pH dependent with a higher degree of cross-linking being observed at pH 6.5 than at pH 7.0.
D003580:Cytochromes / Q000378:metabolism*; D045348:Cytochromes f; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D008970:Molecular Weight; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000378:metabolism; D010970:Plastocyanin / Q000378:metabolism*; D011485:Protein Binding; D012967:Sodium Dodecyl Sulfate
D003580:Cytochromes; D046911:Macromolecular Substances; D010940:Plant Proteins; D012967:Sodium Dodecyl Sulfate; D010970:Plastocyanin; D045348:Cytochromes f