[Antioxidants as stabilizers of cytochrome P-450 in hepatocytes].
Spontaneous destruction of cytochrome P-450 arising from activation of lipid peroxidation (LPO) occurs during incubation of hepatocytes. LPO activation in hepatocyte suspension by a catalytic system containing Fe2+--ADP plus NADP X H makes the destruction of cytochrome P-450 more rapid. Supplementation of the incubation medium with the antioxidant, 2-ethyl-6-methyl-3-hydroxypyridine (HP-6), inhibits LPO, on the one hand, and stabilizes cytochrome P-450, on the other one. Ionol appeared to be a more effective LPO inhibitor in hepatocytes and, accordingly, a more effective stabilizer of cytochrome P-450 than water-soluble HP-6.
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats
D000975:Antioxidants; D008054:Lipid Peroxides; D008315:Malondialdehyde; D003577:Cytochrome P-450 Enzyme System
Antioksidanty kak stabilizatory tsitokhroma P-450 v gepatotsitakh.