[Effect of the pH on the membrane resting potentials of the muscle fibers in the frog].
The pH change from 7.2 to 8.2 causes a decrease of resting membrane potential (RMP) of muscle fibers in the frog at 2.5 mM/l of potassium ions and does not affect the RMP at 5.0 mM/l in the Ringer solution. Tetrodotoxin or tetraethylammonium do not prevent the membrane depolarization after the pH change, but a lowering of temperature, ouabain or a decrease of chloride ions concentration do prevent it. Muscle denervation or blockade of axoplasmic flow by colchicine with no impairment of neuro-muscular transmission, cause a decrease of RMP of muscle fibers, and a pH change from 7.2 to 8.2 induces a hyperpolarization of the muscle membrane under these conditions. Ouabain or a lowering of temperature prevent the membrane hyperpolarization after a pH change. The data obtained suggest the possibility of chloride or proton-chloride pump existence in the frog muscle membrane and that the neurotrophic regulation of this probable mechanism is connected with axoplasmic flow.
D000818:Animals; D002713:Chlorine / Q000502:physiology; D003714:Denervation; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D009132:Muscles / Q000187:drug effects / Q000502:physiology*; D011895:Rana ridibunda; D013696:Temperature
D002713:Chlorine
Vliianie pH na membrannyĭ potentsial pokoia myshechnykh volokon liagushki.