title: Evidence for the involvement of central conversion of testosterone to oestradiol-17 beta in the regulation of luteinizing hormone secretion in the cockerel.
abstract: Treatment of intact cockerels with the synthetic antioestrogen tamoxifen caused a significant increase in the plasma concentration of LH. In contrast, passive immunization with an antiserum raised against oestradiol-17 beta did not lead to an increase in plasma LH. A pronounced depressive effect of injections of 0.1 mg testosterone propionate (TP) or 0.1 mg oestradiol benzoate (OB) on plasma concentrations of LH was prevented by tamoxifen. Furthermore, a pronounced rise in the concentration of LH releasing hormone in the posterior hypothalamus after the injection of cockerels with OB was completely inhibited by tamoxifen. Neither 0.1 nor 0.5 mg androstenedione modified the concentration of LH in plasma. A dose of 0.05 mg TP, which failed to depress the concentration of LH in plasma of intact cockerels, caused a marked fall in plasma LH in castrated cockerels. Tamoxifen itself exhibited weak oestrogen agonist activity in castrated cockerels by causing a reduction in the concentration of LH in plasma. However, tamoxifen prevented any further depressive effect on LH resulting from the injection of TP. These findings suggest that testosterone exerts an inhibitory influence on LH secretion at the central neural level, partially at least, by means of the product of its aromatization, oestradiol-17 beta.
mesh_terms: D000818:Animals; D002369:Castration; D002645:Chickens; D004958:Estradiol / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D005246:Feedback; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D007106:Immune Sera / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000378:metabolism*; D008297:Male; D013629:Tamoxifen / Q000494:pharmacology; D013739:Testosterone / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D007106:Immune Sera; D013629:Tamoxifen; D013739:Testosterone; D004958:Estradiol; D007986:Luteinizing Hormone