Purification and partial characterization of chick intestinal sucrase.
The sucrase was purified from the small intestinal mucosa of the adult chick. Purification procedure involved solubilization with papain, ethanol precipitation, chromatography on Sephadex G-200 and DEAE-Sephadex. Several characters of the chick intestinal sucrase resembled those of the intestinal sucrase-isomaltase complex of some mammals (rabbit, rat and human).
D000818:Animals; D002645:Chickens; D006863:Hydrogen-Ion Concentration; D007421:Intestine, Small / Q000201:enzymology*; D007700:Kinetics; D008970:Molecular Weight; D012964:Sodium / Q000494:pharmacology; D013379:Substrate Specificity; D013393:Sucrase / Q000302:isolation & purification* / Q000378:metabolism
D012964:Sodium; D013393:Sucrase