Melanin endocytosis by cultured mammalian cells. A model for melanin in a cellular environment.
The incorporation of natural eumelanin from bovine eyes and synthetic 3,4-dihydroxy-phenylalanine (dopa) melanin into Chinese hamster ovary (CHO) cells is reported. The process is linear for at least 8 h. Electron microscopy showed phagocytosis of melanin, either as a single granule or in groups of granules, into cell lysosomes with subsequent degradation of the granule. The general features of the ingestion and degradation processes mimic those of the incorporation of melanosomes into keratinocytes. CHO cells with ingested melanin in general revealed properties very similar to those of the pigment-free CHO cell: cell division, oxygen consumption and plating efficiency were not greatly altered by moderate concentrations of pigment. This suggests that the CHO cell system may be useful for the study of pigment in a cellular environment; pigment-free CHO cells are well characterized and can serve as a good control. Preliminary applications are reported: demonstrations of (1) incorporation of metal ions (Al3+) into CHO cells using melanin as a carrier; (2) the ability of melanin to enhance the rate of oxygen consumption during photo-irradiation of the cells.
D000535:Aluminum / Q000378:metabolism; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004705:Endocytosis; D005260:Female; D008027:Light; D008247:Lysosomes / Q000378:metabolism; D008543:Melanins / Q000378:metabolism* / Q000494:pharmacology; D008954:Models, Biological; D010053:Ovary; D010101:Oxygen Consumption / Q000187:drug effects; D014466:Ultraviolet Rays
D008543:Melanins; D000535:Aluminum