Increase of the yield of DNA from barley nuclei in ethylene glycol by gamma-irradiation.
After gamma-irradiating the nuclei isolated from dry seed-embryo of barley in the presence of varying concentrations of ethylene glycol, macromolecular DNA (deoxyribonucleic acid) was extracted from them. The yields of DNA was determined by the 2 methods. The both method of determination on DNA showed a similar tendency; the higher the exposed dose and the concentration of ethylene glycol, the better the yields.
D002467:Cell Nucleus / Q000032:analysis* / Q000528:radiation effects*; D004247:DNA / Q000032:analysis*; D002523:Edible Grain / Q000032:analysis*; D005026:Ethylene Glycols / Q000494:pharmacology*; D005720:Gamma Rays; D001467:Hordeum / Q000032:analysis*; D012639:Seeds / Q000528:radiation effects*
D005026:Ethylene Glycols; D004247:DNA