Anti-human choline acetyltransferase fragments antigen binding (FAB)-sepharose chromatography for enzyme purification.
Fragments Antigen Binding (FAB)-Sepharose immunoaffinity chromatography was successfully applied to the purification of human choline acetyltransferase. The mild elution conditions made it possible to obtain the enzyme in its full catalytic state. The method should be generally applicable to enzyme purification.
D000818:Animals; D002795:Choline O-Acetyltransferase / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D011817:Rabbits
D007140:Immunoglobulin Fab Fragments; D002795:Choline O-Acetyltransferase