title: Measurement of the enzymes lactate dehydrogenase and creatine kinase using reflectance spectroscopy and reagent strips.
abstract: Two new methods for the assay of total activities of lactate dehydrogenase and creatine kinase are described, in which the enzyme activities are measured from a solid-state reagent strip during a kinetic reaction, the reaction being monitored in the ultra-violet region of the spectrum by reflectance spectroscopy. The performances of these methods are evaluated, and compared to conventional "wet" chemistry methods. The solid-phase reagent methods demonstrated precision and accuracy acceptable for diagnostic purposes, and were easy to use by trained operators.
mesh_terms: D004796:Clinical Enzyme Tests / Q000379:methods*; D003402:Creatine Kinase / Q000097:blood*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D011934:Reagent Strips; D013057:Spectrum Analysis
chemical_list: D011934:Reagent Strips; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase