Co-purification of coenzyme-dependent enzymes by affinity chromatography.
Recent developments in general ligand affinity chromatography have greatly facilitated the purification of numerous coenzyme-dependent enzymes in practical scale. By immobilizing various derivatives of coenzymes (NAD+, NADP+, ATP and coenzyme A) on Sepharose, these general ligand affinity columns have the potential of enriching as many as 30% of the two thousand known enzymes as well as their isozymic forms, if they are applied in combination with the conventional procedures.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity / Q000379:methods*; D003067:Coenzymes; D004330:Drosophila / Q000201:enzymology; D004798:Enzymes / Q000302:isolation & purification*; D006593:Hexokinase / Q000302:isolation & purification; D007527:Isoenzymes / Q000302:isolation & purification; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification; D008024:Ligands; D051379:Mice; D010735:Phosphoglycerate Kinase / Q000302:isolation & purification
D003067:Coenzymes; D004798:Enzymes; D007527:Isoenzymes; D008024:Ligands; D007770:L-Lactate Dehydrogenase; D006593:Hexokinase; D010735:Phosphoglycerate Kinase