[Effect of actinomycin D, cycloheximide and hypertonic concentrations of NaCl on the reproduction of the tick-borne encephalitis virus].
Synthesis of virus-specific polypeptides occurs in pig embryo kidney cells infected with tick-borne encephalitis virus and treated with actinomycin D, cycloheximide, and hypertonic concentrations of NaCl in various combinations. Despite the fact that virus-specific matrix RNAs function under such conditions, production of infectious virus particles is inhibited considerably. Virus yield is greatly decreased by treatment with actinomycin D alone and by combined effect of actinomycin D and cycloheximide. Sodium chloride and combined treatment of the infected cells with cycloheximide and NaCl do not affect the intact virus production. The mechanism of the inhibiting effect of actinomycin D on production of infectious tick-borne encephalitis virus is assumed to consist in the impairment of the synthesis of virus-specific ribonucleic acid and/or in marked reduction of its infectivity. Actinomycin D may possibly inhibit somehow the morphogenesis of intact virions of tick-borne encephalitis virus.
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology*; D003609:Dactinomycin / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian; D004669:Encephalitis Viruses, Tick-Borne / Q000187:drug effects*; D010452:Peptide Biosynthesis; D010455:Peptides / Q000032:analysis; D012462:Saline Solution, Hypertonic / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology*; D013552:Swine; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects*
D010455:Peptides; D012462:Saline Solution, Hypertonic; D003609:Dactinomycin; D012965:Sodium Chloride; D003513:Cycloheximide
Vliianie aktinomitsina D, tsiklogeksimida i gipertonicheskikh kontsentratsiĭ NaCl na reproduktsiiu virusa kleshchevogo éntsefalita.