title: Plasmid replication in a temperature-sensitive chromosome replication mutant of Staphylococcus aureus.
abstract: Replication of the antibiotic resistance plasmids pI258, pT10501 and pC221 has been investigated in a mutant of Staphylococcus aureus NCTC 8325, which is temperature-sensitive for the initiation of chromosome replication. Replication of pI258 stopped rapidly at the nonpermissive temperature, whilst replication of pT10501 and pC221 continued (although at a lower rate than in the wild-type). It is proposed that the product of the mutant gene may be required directly for pI258 replication, but not for replication of pT10501 or pC221.
mesh_terms: D002876:Chromosomes, Bacterial / Q000502:physiology; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D009154:Mutation; D011815:R Factors; D013211:Staphylococcus aureus / Q000235:genetics*; D013696:Temperature
chemical_list: D004269:DNA, Bacterial