Effects of alpha-trichosanthin and alpha-momorcharin on the development of peri-implantation mouse embryos.
alpha-Trichosanthin (0.3 mg/25 g) and alpha-momorcharin (0.2 mg/25 g) given intraperitoneally to mice on Days 4 and 6 of pregnancy led to an inhibition of implantation. In-vitro study of the effects of these proteins on developing mouse embryos showed that these proteins did not affect the transformation of morulae to blastocysts but further development was impaired: many blastocysts failed to hatch from the zona, the incidence of successful attachment to a plastic substrate decreased and the extent of trophoblastic outgrowth diminished. Inner cell mass development was less affected than was the trophoblast. The in-vivo inhibition of implantation may therefore be a consequence of the deleterious effect of these proteins on the trophoblast.
D000019:Abortifacient Agents / Q000494:pharmacology*; D000020:Abortifacient Agents, Nonsteroidal / Q000494:pharmacology*; D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D010064:Embryo Implantation / Q000187:drug effects*; D005260:Female; D007274:Injections, Intraperitoneal; D051379:Mice; D008813:Mice, Inbred ICR; D010940:Plant Proteins / Q000494:pharmacology*; D011247:Pregnancy; D012269:Ribosomal Proteins; D054788:Ribosome Inactivating Proteins; D015978:Trichosanthin; D014327:Trophoblasts / Q000187:drug effects
D000019:Abortifacient Agents; D000020:Abortifacient Agents, Nonsteroidal; D010940:Plant Proteins; D012269:Ribosomal Proteins; D015978:Trichosanthin; C039582:MMC protein, Momordica charantia; D054788:Ribosome Inactivating Proteins