Inhibition of antibody binding to viable cells by liposomes containing purified rat spleen glycoprotein.
The capacity of liposomes with inserted RT-1 histocompatibility antigen to bind anti-RT-1 antibodies varies depending on their lipid composition and mode of preparation. The binding capacity of liposomes prepared by dialysis is different from that of liposomes prepared by gel filtration or of small membrane protein micelles. A new assay for analysis of membrane antigens has been developed. Liposomes are used to compete for antibody binding with in vitro cultured cells while binding of antibody to the same adherent liposomes is simultaneously assayed.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002460:Cell Line; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000032:analysis / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D008081:Liposomes / Q000032:analysis / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology*; D009994:Osmolar Concentration; D011817:Rabbits; D051381:Rats; D011914:Rats, Inbred BN; D011920:Rats, Inbred WF; D013154:Spleen / Q000032:analysis
D000954:Antigens, Surface; D006023:Glycoproteins; D006649:Histocompatibility Antigens; D008081:Liposomes; D008565:Membrane Proteins