Optimal enrichment time for isolation of Vibrio parahaemolyticus from seafood.
The growth of Vibrio parahaemolyticus in a liquid medium was compared with that of human fecal flora and estuarine flora. No marked differences were noted between growth at 25 and 37 degrees C for V. parahaemolyticus. However, the marine organisms were strongly inhibited when incubated at 37 degrees C. Incubation for 8 h in an enrichment broth yielded V. parahaemolyticus growth, even with a small inoculum, whereas the marine and fecal floras were inhibited. Therefore, enrichment for 8 h at 37 degrees C appears to be optimal for isolation of V. parahaemolyticus, permitting more rapid results in seafood analysis.
D000818:Animals; D005243:Feces / Q000382:microbiology; D005399:Fishes / Q000382:microbiology*; D005516:Food Microbiology; D006801:Humans; D012623:Seawater; D012758:Shellfish; D013696:Temperature; D013997:Time Factors; D014736:Vibrio parahaemolyticus / Q000254:growth & development / Q000302:isolation & purification*