Polynucleotide-histone H1 complexes as probes for blot hybridization.
Biotin and/or 125I-labelled histone H1 proteins (21 kd) have been covalently bound to single-stranded DNA. Complexes of equal masses of DNA and modified histone H1 (approximately 1 histone H1 molecule per 70 nucleotides) were used as probes for blot hybridization experiments and found to have hybridization characteristics very similar (or identical) to radiolabelled DNA probes.
D001710:Biotin; D004277:DNA, Single-Stranded; D006657:Histones; D009692:Nucleic Acid Heteroduplexes; D009693:Nucleic Acid Hybridization; D011485:Protein Binding
D004277:DNA, Single-Stranded; D006657:Histones; D009692:Nucleic Acid Heteroduplexes; D001710:Biotin