Neutron scattering studies of nucleosome structure at low ionic strength.
Ionic strength studies using homogeneous preparations of chicken erythrocyte nucleosomes containing either 146 or 175 base pairs of DNA show a single unfolding transition at about 1.5 mM ionic strength as determined by small-angle neutron scattering. The transition seen by some investigators at between 2.9 and 7.5 mM ionic strength is not observed by small-angle neutron scattering in either type of nucleosome particle. The two contrasts measured (H2O and D2O) indicate that only small conformational changes occur in the protein core, but the DNA is partially unfolded below the transition point. Patterson inversion of the data and analysis of models indicate that the DNA in both types of particle is unwinding from the ends, leaving about one turn of supercoiled DNA bound to the histone core in approximately its normal (compact) conformation. The mechanism of unfolding appears to be similar for both types of particles and in both cases occurs at the same ionic strength. The unfolding observed for nucleosomes in this study is in definite disagreement with extended superhelical models for the DNA and also disagrees with models incorporating an unfolded histone core.
D000818:Animals; D001482:Base Composition; D002645:Chickens; D004247:DNA / Q000097:blood; D004277:DNA, Single-Stranded / Q000302:isolation & purification; D004912:Erythrocytes / Q000648:ultrastructure; D008957:Models, Genetic; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000648:ultrastructure*; D009994:Osmolar Concentration
D004277:DNA, Single-Stranded; D009707:Nucleosomes; D004247:DNA