Protein synthesis in the uterus of the immature rat: the effects of short exposures to oestradiol-17 beta.
Three methods were used to study the effect of oestrogen on the incorporation of radioactive precursor into uterine protein. Intact tissue was incubated in vitro. Isolated uterine epithelial, stromal and myometrial cells were labelled in vitro. Isolated polysomes were translated in cell free protein synthesising systems. In all of these systems, minor qualitative changes in protein synthesis were detected where the uteri were derived from oestrogen-treated rats. These changes were most dramatic in isolated stromal cells and were accompanied by a marked overall increase in protein synthesis. The translation of mRNA on isolated uterine polysomes revealed a sequence of minor, but reproducible, oestradiol-induced changes. It was difficult, however, to relate these changes to those detected in incubated tissue or cells, possibly because the cell free translation products were not subject to normal post-translational modification and processing.
D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D010452:Peptide Biosynthesis; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats; D013997:Time Factors; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D004958:Estradiol