[Preparation of leukocyte-poor erythrocyte concentrates using a simple aspiration technic].
Depletion of leukocytes from red blood cells can prevent transfusion reactions in HLA-sensitized patients. We describe a simple technique of aspiration of the buffy-coat after centrifugation of red blood cells concentrates, less than 6 days old. This method can remove 87% of the leukocytes. The average leukocyte count after aspiration is 2 x 10(8). This method is inexpensive and does not require any special equipment.
D001799:Blood Sedimentation; D001803:Blood Transfusion; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation; D017707:Erythrocyte Transfusion; D006801:Humans; D007962:Leukocytes
Préparation de concentrés érythrocytaires appauvris en leucocytes par technique simple d'aspiration.