title: In vitro effects of fructose-1,6-diphosphate on oxygen affinity and electrolyte equilibrium in human blood.
abstract: The in vitro effects of a solution of fructose-1,6-diphosphate on hemoglobin-oxygen affinity and electrolyte distribution between plasma and red cells were evaluated. Fructose-1,6-diphosphate increased intracellular pH and decreased extracellular pH. There were no shifts in Na+, K+, Ca++ and Cl- between plasma and red cells. Red cell content of 2,3-diphosphoglycerate remained unchanged, while P50 was reduced (as a consequence of the intracellular alkalosis). The therapeutical implications of these results are discussed.
mesh_terms: D019794:2,3-Diphosphoglycerate; D004163:Diphosphoglyceric Acids / Q000097:blood; D004573:Electrolytes / Q000097:blood*; D005635:Fructosediphosphates / Q000494:pharmacology*; D006019:Glycolysis / Q000187:drug effects; D006598:Hexosediphosphates / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010100:Oxygen / Q000097:blood*; D010710:Phosphates / Q000494:pharmacology
chemical_list: D004163:Diphosphoglyceric Acids; D004573:Electrolytes; D005635:Fructosediphosphates; D006598:Hexosediphosphates; D010710:Phosphates; D019794:2,3-Diphosphoglycerate; C029063:fructose-1,6-diphosphate; D010100:Oxygen