Isolation of goat spleen phosphonolipids by thin-layer chromatography: their identification and silicic acid column chromatographic separation.
Goat spleen phosphonolipids were isolated by preparative thin-layer chromatography (TLC) using the solvent system methanol--water (2:1) and were identified by TLC, nitrogen--phosphorus determinations and IR spectroscopy. Silicic acid column chromatography of the isolated phosphonolipids confirmed the initial assignments.
D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D006041:Goats; D010743:Phospholipids / Q000302:isolation & purification*; D012824:Silicic Acid; D013055:Spectrophotometry, Infrared / Q000379:methods; D013154:Spleen / Q000032:analysis*
D010743:Phospholipids; C028299:phosphonolipids; D012824:Silicic Acid