Extraction of androgen-receptor complexes from regions of rat ventral prostate nuclei sensitive or resistant to nucleases.
Rat ventral prostate nuclei contain androgen-binding sites which are susceptible or resistant to excision by endonucleolytic action. Those which were susceptible were associated both with oligonucleosomal and subnucleosomal particles. The sedimentation profile characteristic of a nuclear androgen-receptor complex could be obtained by exhaustive nucleolytic digestion or by treatment of fractions with KCl (0.6 mol/l). Androgen-binding sites resistant to DNAase I were also resistant to KCl, whereas those sites resistant to micrococcal nuclease were partially extractable with KCl. Nuclease-resistant sites could be extracted with heparin (10 mg/ml). Androgen-receptor complexes obtained from nuclease-sensitive or nuclease-resistant regions by extraction with KCl or heparin were indistinguishable by routine sedimentation analysis.
D000818:Animals; D001665:Binding Sites; D002369:Castration; D002467:Cell Nucleus / Q000032:analysis* / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism; D008297:Male; D011467:Prostate / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D011944:Receptors, Androgen / Q000302:isolation & purification* / Q000378:metabolism; D011987:Receptors, Steroid / Q000302:isolation & purification*; D013347:Subcellular Fractions
D011944:Receptors, Androgen; D011987:Receptors, Steroid; D013196:Dihydrotestosterone; D003851:Deoxyribonucleases