Re-examination of the DNA-mediated transformation in goldfish.
DNAs were isolated from goldfish, and the balancer bearing species of newt. They were injected separately into fertilized goldfish eggs. A balancer developed in some newt-DNA injected goldfish. DNA injected goldfish eggs occasionally developed a non-balancer process. These results demonstrated the newt DNA-mediated transfer of the species character from newt to goldfish. The injected DNA was localized by the method of autoradiography using H3-cDNA. Silver grains were found in areas over the colchicine induced metaphase. The incorporation of foreign DNA into chromosomes provides a material basis for genetic transformation.
D000818:Animals; D001345:Autoradiography; D002454:Cell Differentiation; D003530:Cyprinidae / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D006054:Goldfish / Q000196:embryology / Q000235:genetics*; D011817:Rabbits; D012455:Salamandridae / Q000235:genetics; D013045:Species Specificity
D004247:DNA