PAGE-autoradiography of fucose incorporation into rat hippocampal glycoproteins after acquisition of a brightness discrimination.
Male rats aged 8 weeks received intraventricular injections of 100 microCi (3.7 MBq) L-[1-3H] fucose each, 7 h after acquisition of a brightness discrimination task. Two, 8 and 24 h as well as 16, 29 and 60 days after injection of labelled fucose hippocampal tissue was prepared to obtain Tris-soluble, Triton-soluble and Triton-resistant fractions. Two and 8 h after application of [3H]fucose, the trained animals revealed an increased incorporation of fucose mainly into the Triton-soluble glycoproteins. However, it is considered that also material from the Tris-soluble fraction may be used as an additional precursor for Triton-soluble glycoproteins. Quantitative analysis of autoradiographic densitograms obtained after SDS-polyacrylamide gel electrophoresis showed the occurrence of a training-related increase in fucose incorporation predominantly into the slow-moving Triton-soluble glycoproteins. The latter exhibited a higher turnover rate than the faster-moving glycoproteins. For the longer incorporation times (days) after injection of labelled fucose, no differences were observed between trained animals and corresponding active and passive controls.
D000818:Animals; D001345:Autoradiography; D003216:Conditioning, Operant; D004192:Discrimination, Psychological; D004591:Electrophoresis, Polyacrylamide Gel; D005643:Fucose / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis* / Q000302:isolation & purification; D006624:Hippocampus / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D010775:Photic Stimulation; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D006023:Glycoproteins; D014316:Tritium; D005643:Fucose