Molecular weight determination of peptides by high-performance gel permeation chromatography.
A method for molecular weight determination of small peptides using Bio-Sil TSK 20 and Bio-Gel TSK 125 columns is described. The TSK 20 column provided a good separation of the standard peptides in the range from 1000-10,000 with an accuracy of less than 5% from the calculated regression line. Two combined TSK 125 columns allowed a reliable molecular weight determination in the range from 800 to 3500.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification*
D010455:Peptides