Differential substrate oxidation by dissociated brain cells and homogenates during development.
The rates of oxidation of 3-hydroxy[3-14C]butyrate, [3-14C]acetoacetate and [6-14C]glucose were compared by using two different preparations of brain from the same animals (i.e. whole homogenates and dissociated brain cells) at various ages during development. In homogenates the rates of oxidation of 3-hydroxy[3-14C]butyrate and [3-14C]acetoacetate were high in young rats and low in adults, and were significantly higher at most ages during development than those obtained for intact cells. In contrast, rates of [6-14C]glucose oxidation by homogenates and intact cells were essentially the same at early ages; however, the rate by homogenates did not change throughout development, whereas that by intact cells increased severalfold by adulthood. In adult animals the initial glucose concentration affected the rate of glucose oxidation in homogenates, but not in intact cells. These data suggest a role for the intact cell membrane in the regulation of alternative substrate utilization by brain cells and that this process changes during development. However, the data may reflect selective differences in the cellular and subcellular components in these two preparations.
D020155:3-Hydroxybutyric Acid; D000090:Acetoacetates / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000378:metabolism; D006885:Hydroxybutyrates / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains
D000090:Acetoacetates; D006885:Hydroxybutyrates; C016635:acetoacetic acid; D005947:Glucose; D020155:3-Hydroxybutyric Acid