Isolation of thymosin alpha 1 from thymosin fraction 5 of different species by high-performance liquid chromatography.
High-performance liquid chromatography (muBondapak C18 column with 0.05% trifluoroacetic acid in acetonitrile as solvent system) was used to isolate thymosin alpha 1 (alpha 1) from thymosin fraction 5 (f5) of various species (calf, pig, sheep and mouse). Each of the f5 preparations gave a protein peak similar in retention time to bovine thymosin alpha 1. This peak coincided with the immunoreactive peak determined by a radioimmunoassay for alpha 1. Chromatographic analysis of fresh thymus tissue extracts using a high-performance liquid chromatographic similar system did not reveal a detectable protein peak or immunoreactive peak at the alpha 1 position. Our results suggest that alpha 1 may be synthesized in a precursor form in animal tissues.
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D051379:Mice; D011863:Radioimmunoassay; D051381:Rats; D013552:Swine; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D013950:Thymus Gland / Q000032:analysis*
C033782:thymosin fraction 5; D013947:Thymosin; D000077596:Thymalfasin