Use of antioxidants in the analysis of vitamins A and E in mammalian plasma by high performance liquid chromatography.
A simple, sensitive, quantitative method for the simultaneous assay of retinol, alpha-tocopherol and gamma-tocopherol in rat, guinea pig, monkey and human plasma was developed by using high performance liquid chromatography. It was found that antioxidant was required to stabilize the fat-soluble vitamins in the plasma of rats. The effect of several antioxidants on the recovery of fat-soluble vitamins was evaluated. Results showed that 0.125% butylated hydroxytoluene (BHT) in ethanol and 0.025% BHT in heptane yielded recoveries greater than 95% in 0.1 ml plasma.
D000328:Adult; D000818:Animals; D000975:Antioxidants; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D008253:Macaca mulatta; D008297:Male; D051381:Rats; D014801:Vitamin A / Q000097:blood*; D014810:Vitamin E / Q000097:blood*
D000975:Antioxidants; D014801:Vitamin A; D014810:Vitamin E