A new mass screening method for determining UDP-galactose in blood.
We devised a new microfluorometric mass screening method for determining UDP-galactose by using only one blood disc of 3 mm in diameter. This method contains two coupling enzymes of UDP-galactose-4-epimerase and UDP-glucose dehydrogenase, and conversion of NAD to NADH. The assay ranges are 0 to 1 mM or 0 to 0.2 mM UDP-galactose in blood. UDP-galactose contents were determined in galactosemia of epimerase-, transferase- and kinase-deficiencies, and several cases with high or low Paigen's values.
D005693:Galactosemias / Q000097:blood / Q000235:genetics / Q000517:prevention & control*; D006580:Genetic Carrier Screening; D006801:Humans; D008403:Mass Screening / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods; D014531:Uridine Diphosphate Galactose / Q000097:blood*; D014539:Uridine Diphosphate Sugars / Q000097:blood*
D014539:Uridine Diphosphate Sugars; D014531:Uridine Diphosphate Galactose