The identity of bovine liver "nothing dehydrogenase".
An assay system was developed which allowed the partial purification of the substrate for beef liver nothing dehydrogenase. This was also found to be a substrate for lactate dehydrogenase (LDH). Using electrophoretic separations of purified LDH and beef liver extracts, nothing dehydrogenase was determined to be primarily the H4 isoenzyme of LDH. The nothing dehydrogenase phenomena was also observed with highly-purified, commercially-obtained LDH.
D000818:Animals; D002417:Cattle; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D009243:NAD / Q000378:metabolism; D013379:Substrate Specificity; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D007527:Isoenzymes; D013439:Sulfhydryl Reagents; D009243:NAD; D007770:L-Lactate Dehydrogenase