[Application of ultrafiltration for concentration and purification of Penicillium vitale catalase].
Various factors are studied for their effect on the ultrafiltration of Penicillium vitale catalase. It is established that acetate cellulose and polyamide membranes may be used for additional purification and deep concentration of native preliminarily purified enzyme solutions. Membranes YAM-300 and YAM-450 are most preferable. At the temperature of 10 degrees C, pressure 0.2 MPa and 100-fold concentration of the catalase solution the enzyme yield at the ultrafiltration stage is 96-100%.
D002374:Catalase / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D010407:Penicillium / Q000201:enzymology*; D014462:Ultrafiltration / Q000379:methods
D002374:Catalase
Primenenie ul'trafil'tratsii dlia kontsentrirovaniia i ochistki katalazy Penicillium vitale.