Determination of conjugated bile acids in human bile by isotachophoresis in a non-aqueous solvent using a.c. conductivity and UV detection.
A method for the determination of conjugated bile acids in human bile using isotachophoresis in 95% methanol is described. The leading ion is 0.01 M chloride, the counter ion is hydroxylamine at its pK value and the terminating ion is N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid (HEPES). The sample preparation consists of C18-silica cartridge adsorption. Microlitre amounts of the methanol eluate are injected and analysed within 20 min in a 0.2 mm I.D. PTFE capillary. The sensitivity of the method is better than 50 ng of each of the conjugated bile acids using a.c. conductivity detection.
D001646:Bile / Q000032:analysis*; D001647:Bile Acids and Salts / Q000302:isolation & purification*; D004553:Electric Conductivity; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012997:Solvents
D001647:Bile Acids and Salts; D012997:Solvents