Biochemistry of the ageing rat lens. II. Isoelectric focusing of water-soluble crystallins.
Thin-layer isoelectric focusing of the water-soluble crystallins shows with increasing age of the animals an increase of alpha-crystallins of high and low molecular weight and of the total beta-crystallins content. The beta-crystallin components of high molecular weight also increased during ageing, but the beta-crystallins of lower molecular weight decreased considerably. The total gamma-crystallin content, as well as certain gamma-crystallin components also decreased considerably with increasing age, or vanished completely. From 429 to 1,211 days of age the male gamma-crystallins as percent of the water-soluble moiety, were higher than the female gamma-crystallins. As a consequence of ascending age, the content of water-insoluble proteins of the rat lens increased gradually and continuously, e.g. by a process of insolubilization of soluble beta- and gamma-crystallins.
D000375:Aging; D000818:Animals; D003459:Crystallins / Q000032:analysis*; D005260:Female; D007525:Isoelectric Focusing; D007908:Lens, Crystalline / Q000032:analysis / Q000502:physiology*; D008297:Male; D008970:Molecular Weight; D051381:Rats; D012737:Sex Factors; D012995:Solubility
D003459:Crystallins