Detection of leukotriene C4 and D4 in the exudate of rat carrageenin-induced pleurisy.
Rat carrageenin-induced pleurisy was used as an acute exudative inflammatory model. The crude ethanol extract of the pleural fluid at 5 hr after carrageenin injection caused the very slow contraction of guinea-pig ileum, which was antagonized by FPL 55712 (1 microgram/ml). The ethanol extract was cleaned by LH-20 and was rendered for separation of LTC4 and LTD4 by reversed-phase high-performance liquid chromatography (HPLC). Two peaks which showed the same retention time on HPLC as those of LTC4 and LTD4 had the contractile activity of guinea-pig ileum and the ratios of the contractile activity to the height on HPLC agreed with those of synthetic LTC4 and LTD4. Two peaks of delta 6-trans-LTB4, 5S, 12R-(E, E, E, Z)-diHETE and 5S, 12S-(E, E, E, Z)-diHETE, were detected, but the appreciable amount of LTB4 was smaller than that of each delta 6-trans-LTB4 in the pleural fluid at 5 hr.
D000818:Animals; D001681:Biological Assay; D002351:Carrageenan; D002851:Chromatography, High Pressure Liquid; D002867:Chromones / Q000494:pharmacology; D007082:Ileum / Q000187:drug effects; D008297:Male; D010998:Pleurisy / Q000139:chemically induced*; D051381:Rats; D011919:Rats, Inbred Strains; D013189:SRS-A / Q000032:analysis* / Q000494:pharmacology; D013997:Time Factors
D002867:Chromones; D013189:SRS-A; C006312:FPL 55712; D002351:Carrageenan