Specific binding of [3H]oxytocin in the female rat brain.
Because of demonstrated effects of oxytocin on some limbic system mediated behaviours, the specific binding of [3H]oxytocin to a plasma membrane containing fraction of rat limbic tissue has been studied. The binding of the microsomal fraction of estrogenized, female rat tissue was time dependent and saturable, with a Bmax of 2.5 X 10(-13) moles per milligram of protein and an apparent KD of 3.53 X 10(-8)M, and appeared to show positive cooperativity. The pH optimum of the binding was 6.0, close to the pH optimum for oxytocin-neurophysin binding; however, other results show the two types of binding to be different. The microsomal fraction did not appreciably degrade oxytocin under the conditions used for [3H]oxytocin binding. The distribution in limbic tissue of oxytocin-degrading activity and of individual enzymes capable of degrading oxytocin has been examined and an interplay of enzymes concentrated in different cell types is proposed.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D007700:Kinetics; D008032:Limbic System / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010121:Oxytocin / Q000031:analogs & derivatives / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains
D009419:Nerve Tissue Proteins; D010121:Oxytocin