High-performance liquid chromatographic application of the Hummel and Dreyer method for the determination of colchicine-tubulin binding parameters.
An application of the Hummel and Dreyer gel chromatography procedure modified for high-performance liquid chromatography has been used to determine the dissociation constant for the colchicine-tubulin interaction at 25 degrees C. The results obtained are compared with results of other equilibrium and non-equilibrium techniques and demonstrate that the initial interaction of colchicine with tubulin must be rapid and probably reversible. This rapid and sensitive technique, which does not require radioisotopes for measurement of the binding parameters, will be extremely useful for characterization of tubulin-ligand interactions.
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003078:Colchicine / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D007700:Kinetics; D014404:Tubulin / Q000378:metabolism*
D014404:Tubulin; D003078:Colchicine