Effect of long-wave ultraviolet light on the lens. IV. Leucine metabolism in normal human lenses in vitro.
A series of lens pairs (each pair from the same donor) from human eyebank eyes were incubated in a medium containing tracer leucine. One lens of each pair was exposed to long-wave ultraviolet light. The other was shielded. The irradiated lenses accumulated free leucine to a level only 53% of that in the shielded lenses. Irradiation depressed leucine incorporation to 29% of the control level. These results show that UV may adversely affect important parameters of human lens protein metabolism in vitro over a period of 10-30 h.
D000328:Adult; D000368:Aged; D002386:Cataract / Q000209:etiology; D003459:Crystallins / Q000528:radiation effects*; D006801:Humans; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000528:radiation effects*; D007930:Leucine / Q000528:radiation effects*; D008875:Middle Aged; D014466:Ultraviolet Rays / Q000009:adverse effects*
D003459:Crystallins; D007930:Leucine