Characterization studies of glucose dehydrogenase.
Porcine liver beta-D-glucose dehydrogenase has been isolated using Triton X-114 to release it from the endoplasmic reticulum. The purified enzyme contains a limited amount (1.7%) of lipid material, including cholesterol, fatty acids, mono and diglycerides, phosphatidylcholine, phosphatidylethanolamine, and cholesterol esters. This enzyme is a tetrameric protein containing an extensive number of hydrophobic residues. This form of glucose dehydrogenase is capable of turning over both beta-D-glucose and alpha-D-glucose-6-phosphate in vivo as indicated from a steady state kinetic analysis at 37 degrees C.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005260:Female; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000032:analysis / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000201:enzymology*; D008297:Male; D010714:Phosphatidylethanolamines / Q000032:analysis; D013379:Substrate Specificity; D013552:Swine
D000596:Amino Acids; D008055:Lipids; D010714:Phosphatidylethanolamines; D002237:Carbohydrate Dehydrogenases; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase