title: Comparison of flow cytometric analysis and [3H]thymidine incorporation for measurement of the effects of drug toxicity on lymphocyte stimulation.
abstract: Human mononuclear cells were exposed to three antiviral agents, stimulated with phytohemagglutinin, and assayed for DNA synthesis with [3H]thymidine uptake and flow cytometric analysis. Cytosine-arabinoside demonstrated inhibition of blastogenic reactivity by both [3H]thymidine uptake and flow cytometric analysis, whereas acyclovir showed no significant suppression. In contrast, (E)-5-(2-bromovinyl)-2'-deoxyuridine, a thymidine analog, demonstrated a lack of correlation between the two methods. The competitive inhibition of some compounds with [3H]thymidine incorporation necessitates the use of other methods to measure DNA synthesis.
mesh_terms: D001973:Bromodeoxyuridine / Q000031:analogs & derivatives / Q000633:toxicity; D003561:Cytarabine / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D064420:Drug-Related Side Effects and Adverse Reactions; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism*
chemical_list: D010835:Phytohemagglutinins; D003561:Cytarabine; C020235:brivudine; D004247:DNA; D001973:Bromodeoxyuridine; D013936:Thymidine