[Isolation of triglyceridase from the aorta].
Isolation of triglyceridase from pig aorta was carried out by means of a following procedure: preparation of acetone powder, extraction of the enzyme in presence of sodium deoxycholate, ammonium sulfate fractionation, chromatography on Sepharose 6B, DEAE cellulose and cholate-Sepharose. The 1,000-fold purification of the enzyme was achieved. The purified preparation of triglyceridase was free of cholesterol esterase and proteinase activities.
D000818:Animals; D001011:Aorta / Q000201:enzymology*; D002250:Carbon Radioisotopes; D007700:Kinetics; D008049:Lipase / Q000302:isolation & purification*; D008970:Molecular Weight; D013552:Swine
D002250:Carbon Radioisotopes; D008049:Lipase
Vydelenie triglitseridazy aorty.