Distribution of selenium and glutathione peroxidase in blood fractions from humans, rhesus and squirrel monkeys, rats and sheep.
The distribution of selenium and glutathione peroxidase (GSH-Px) was investigated in blood fractions from humans, rhesus and squirrel monkeys, sheep and rats by gel filtration (Sephadex G-150). The majority of selenium in plasma and erythrocytes from sheep, squirrel monkeys and rats cochromatographed with GSH-Px. In contrast, selenium in plasma from humans and rhesus monkeys cochromatographed with two non-GSH-Px proteins and nearly all of the selenium in erythrocytes cochromatographed with the hemoglobin peak. A significant amount of GSH-Px activity in rhesus monkey and human erythrocytes also cochromatographed with the hemoglobin fraction, but very little GSH-Px coeluted with hemoglobin from squirrel monkeys, ovine and rat blood. The results suggest that GSH-Px activity may not be a good measure of selenium status in higher primates.
D000328:Adult; D000818:Animals; D004796:Clinical Enzyme Tests / Q000592:standards; D004912:Erythrocytes / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000097:blood*; D006461:Hemolysis; D006801:Humans; D008253:Macaca mulatta; D008875:Middle Aged; D010949:Plasma / Q000378:metabolism; D051381:Rats; D012453:Saimiri; D012643:Selenium / Q000008:administration & dosage / Q000097:blood* / Q000172:deficiency; D012756:Sheep; D013045:Species Specificity
D005979:Glutathione Peroxidase; D012643:Selenium