title: Preparation of microspheres of poly(glycidyl methacrylate) and its derivatives as carriers for immobilized proteins.
abstract: Microspheres of poly(glycidyl methacrylate) and its derivatives have been prepared. The diameter can be varied in the range from 0.5 to 5 micron. Proteins were immobilized on the microspheres by various processes. The maximum amount of covalently immobilized gamma-globulin on the microspheres was found to be about 6 mg/m2. It was shown that dyed microspheres with a diameter of 2-4 micron are applicable to immunological agglutination tests on a microplate as carrier particles substituting for red blood cells.
mesh_terms: D000179:Acrylates; D000818:Animals; D001799:Blood Sedimentation; D002352:Carrier Proteins; D002417:Cattle; D002645:Chickens; D004852:Epoxy Compounds / Q000378:metabolism; D004988:Ethers, Cyclic; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D008689:Methacrylates / Q000378:metabolism; D008863:Microspheres; D011817:Rabbits; D012756:Sheep
chemical_list: D000179:Acrylates; D002352:Carrier Proteins; D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D007074:Immunoglobulin G; D008689:Methacrylates; C007870:glycidyl methacrylate