Influence of monensin on cation influx and glycolysis of Eimeria tenella sporozoites in vitro.
Extracellular Eimeria tenella sporozoites exposed to 1.0 microgram/ml monensin at 40 C had an accelerated rate of sodium influx as well as an increased rubidium uptake that was inhibited by the cardiac glycoside, ouabain. These results suggested the presence of a functional (Na+-K+)-ATPase and its stimulation by monensin. Under the same conditions, sporozoite ATP concentrations declined, lactate production increased and the rate of amylopectin utilization was enhanced. Exposure to monensin also appeared to stimulate the rate of sporozoite glycolysis. The results of this study demonstrated that the cidal effect of monensin on extracellular sporozoites was caused by the capability of the ionophore to act as a transmembrane sodium carrier.
D000818:Animals; D004539:Eimeria / Q000187:drug effects* / Q000378:metabolism; D005663:Furans / Q000494:pharmacology*; D006019:Glycolysis / Q000187:drug effects*; D066298:In Vitro Techniques; D008985:Monensin / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects; D012413:Rubidium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*
D005663:Furans; D008985:Monensin; D012964:Sodium; D012413:Rubidium