Increased satellite association induced by 5' bromodeoxyuridine treatment of phytohemaglutinin-stimulated blood lymphocytes.
5'-Bromodeoxyuridine (BrdU) present in the course of late S and G2 phases of the cell cycle in PHA-stimulated human lymphocyte cultures causes the despiralization and elongation of some chromosome regions, including short arms of acrocentric chromosomes. BrdU present at a concentration of 250 microM during the last 10 h in lymphocyte cultures from 19 healthy subjects did not affect the number of silver-stained NORs, but raised significantly the number of satellite associations of acrocentric chromosomes. The mere substitution of thymine by BrdU in DNA strands as a reason for increased number of satellite associations seems a less plausible explanation than the modification of DNA-protein complexes of NOR regions, which could alter the degree of their spiralization and cause the increased tendency of acrocentric chromosomes to associate in the subsequent metaphase.
D000293:Adolescent; D000328:Adult; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002503:Centromere; D002875:Chromosomes / Q000648:ultrastructure*; D004276:DNA, Satellite / Q000032:analysis*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure*; D008677:Metaphase; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D010835:Phytohemagglutinins / Q000494:pharmacology
D004276:DNA, Satellite; D010835:Phytohemagglutinins; D001973:Bromodeoxyuridine