[Mechanism of the cytotoxic action of formaldehyde on cultured mammalian cells].
Induction and repair of DNA lesions cell inactivation and repair of potentially lethal damages (PLD) were studied after the treatment of cultured cells with formaldehyde. Formaldehyde induced the appearance of a rapidly sedimentating DNA--membrane complex. This complex may contain up to 50% of choline and no more than 3-5% of leucine or lysine incorporated in the acid insoluble cell fraction, Inhibition of DNA synthesis, induction of single strand DNA breaks and/or alkali-labile sites increased with the raise of formaldehyde concentration. A good correlation is observed between with the raise of formaldehyde concentration. A good correlation is observed between with the raise of formaldehyde concentration. A good correlation is observed between the increasing DNA quantities in the rapid sedimentation complex and the cell lethality.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000032:analysis; D004260:DNA Repair / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005557:Formaldehyde / Q000633:toxicity*; D007739:L Cells / Q000187:drug effects*; D051379:Mice; D013997:Time Factors
D005557:Formaldehyde; D004247:DNA
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