Effect of a collagen substrate on the growth and development of normal and tumorigenic rat urothelial cells.
The growth and morphology of 4 tumorigenic rat urothelial cell lines grown on collagen-coated nylon discs was characterized and compared to normal cells. In contrast to cells cultured on a plastic substrate with or without a thin "nonporous" collagen coating, tumor cells grown on porous collagen-coated nylon discs: 1) grew to greater protein densities; 2) formed tissue structures characteristic for the type of tumor they developed upon back-transplantation; and 3) could be grown and cultured indefinitely without subculturing. Thus, similarly to normal urothelial stratification and differentiation in vitro, tumorigenic cells apparently require a "porous" collagen substrate to allow differentiation analogous to that observed in vivo.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003094:Collagen; D003470:Culture Media; D004847:Epithelial Cells; D005200:FANFT; D066298:In Vitro Techniques; D008297:Male; D008855:Microscopy, Electron, Scanning; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology*; D051381:Rats; D011916:Rats, Inbred F344; D001743:Urinary Bladder / Q000166:cytology
D003470:Culture Media; D005200:FANFT; D003094:Collagen