title: Identification of cytoplasmic estrogen receptors in the accessory sex organs of the rabbit and their comparison to the cytoplasmic estrogen receptor in the epididymis.
abstract: Estrogen receptors are present in cytosol prepared from the accessory sex organs (vesicular gland, proprostate, prostate, bulbourethral gland) of sexually immature and of sexually mature rabbits. The receptor in these organs from animals of both age groups has a sedimentation coefficient of 8-10S on low ionic strength (0.01 M KCl) sucrose gradients. Under high ionic strength (0.4 M KCl) conditions, the receptor sediments at approximately 4S. The cytoplasmic estrogen receptor from the epididymis shows age-dependent changes in its sedimentation coefficient. It is 8S under low ionic strength conditions when prepared from immature rabbits and 4S under identical conditions when prepared from sexually mature animals. Although the dissociation constant of the cytoplasmic estrogen receptor in the immature and mature epididymis and accessory sex organs remains constant during development (approximately 0.1 nM), the number of available cytoplasmic estrogen binding sites declines from about 160 fmoles/mg cytosol protein in the immature rabbit to about 40 fmoles/mg cytosol protein in the adult animal. The estrogen receptor in the accessory sex organs is highly specific, the relative affinities of various potential competitors being: estradiol and estrone = 1, diethylstilbestrol = 0.3, estriol = 0.2, tamoxifen = 0.08, testosterone = 0.0004 and 5 alpha-DHT = 0.00005. Changes with age in the physicochemical characteristics of the estrogen receptor and in the concentration of binding sites suggest that the estrogen receptor may be involved in the development and physiological regulation of the male reproductive tract.
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D003593:Cytoplasm / Q000032:analysis; D013196:Dihydrotestosterone / Q000378:metabolism; D004822:Epididymis / Q000032:analysis*; D004958:Estradiol / Q000378:metabolism; D005837:Genitalia, Male / Q000032:analysis*; D008297:Male; D009994:Osmolar Concentration; D011817:Rabbits; D011960:Receptors, Estrogen / Q000032:analysis* / Q000378:metabolism; D012741:Sexual Maturation; D013379:Substrate Specificity
chemical_list: D011960:Receptors, Estrogen; D013196:Dihydrotestosterone; D004958:Estradiol