Transport activity of mouse spleen lymphocytes after their interaction in vitro with Acholeplasma laidlawii cells.
Transport of two non-metabolized carbohydrates (3-O-methyl-D-glucose, 3-O-MG, and 2-deoxy-D-glucose, 2-DG) into mouse spleen lymphocytes after their interaction with Acholeplasma laidlawii cells has been studied. Incubation of A. laidlawii cells and particularly the liposomes prepared from A. laidlawii membrane lipids enhances the rates of the both carbohydrates transport. This treatment resulted in increasing of the Vmax values of 3-O-MG and 2-DG without changing the Km values. This stimulation can be explained by the increasing of the mobility of membrane carbohydrates carriers as a result of the exchange of lipid components between Acholeplasma and lymphocyte membranes. Actually, it has been shown that liposomes derived from A. laidlawii cells grown on the medium with great amount of unsaturated oleic acid stimulate the transport activity more actively than liposomes prepared from the cells grown on the medium with bovine serum or with oleic acid plus cholesterol. It should be suggested that an activation of carbohydrates transport into lymphocytes caused by alteration of the carriers lipid microenvironment.
D019325:3-O-Methylglucose; D000128:Acholeplasma laidlawii / Q000502:physiology*; D000818:Animals; D001692:Biological Transport; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D008081:Liposomes; D008214:Lymphocytes / Q000378:metabolism* / Q000382:microbiology; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000378:metabolism; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D051379:Mice; D013154:Spleen / Q000166:cytology
D003837:Deoxy Sugars; D008081:Liposomes; D008563:Membrane Lipids; D008757:Methylglucosides; D008759:Methylglycosides; D019325:3-O-Methylglucose; D003847:Deoxyglucose