Utilization of conical equilibrium dialysis cells to shorten equilibration time.
Equilibrium dialysis is commonly used to characterize the binding properties of macromolecules; however, it is not always preferred because of the lengthy time required to reach equilibrium. Consequently, other methods have been developed. This paper compares the disadvantages of various methods for obtaining binding data and shows that utilization of a conically shaped chamber can accelerate the process of equilibrium dialysis. For cylindrical and conical cells of different dimensions a linear relationship was found for the time of equilibration versus the ratio of volume to surface area. Minimizing the volume to surface area ratio by using a conical chamber shortens the time of equilibration by more than half and facilitates sampling of the chambers' contents.
D003956:Dialysis / Q000295:instrumentation*; D046911:Macromolecular Substances; D011485:Protein Binding; D013499:Surface Properties
D046911:Macromolecular Substances