[Characteristics of formaldehyde reactions with nucleic acids and their structural components in the presence of primary or secondary amines].
Peculiarities of reversible interaction of formaldehyde with DNA, adenine and some cytosine derivatives in the presence of primary or secondary amines have been studied. Different characters of the examined formaldehyde reactions have been demonstrated. Thus, melting of DNA by formaldehyde at neutral pH is accelerated only in the presence of primary amines. Using UV-spectra data analysis it was concluded that products of cytidine and adenine modification by formaldehyde or methylol derivatives of secondary amines have similar structure. Cytidine and adenine modifications when treated with formaldehyde and primary amine mixture were considerably different and resulted in products with different structures. This is probably related to interactions within two positions of cytosine or adenine residues. A hypothetical scheme of formation and structure of modification products are considered.
D000225:Adenine; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003562:Cytidine; D004247:DNA; D004123:Dimethylamines; D005557:Formaldehyde; D007700:Kinetics; D008744:Methylamines; D009691:Nucleic Acid Denaturation; D012474:Salmon; D013053:Spectrophotometry
D004123:Dimethylamines; D008744:Methylamines; D005557:Formaldehyde; D003562:Cytidine; D004247:DNA; D000225:Adenine
Osobennosti reaktsiĭ formal'degida s nukleinovymi kislotami i ikh strukturnymi komponentami v prisutstvii pervichnykh ili vtorichnykh aminov.