Histochemical demonstration of asparagine-linked oligosaccharides in glycoproteins of human placenta and umbilical cord tissues by means of almond glycopeptidase digestion.
Almond glycopeptidase is an enzyme which cleaves specifically beta-aspartylglucosylamine linkages in glycoproteins with asialo-carbohydrate moieties. With this enzyme, it was possible to demonstrate the localization of asparagine-linked oligosaccharides in glycoproteins of human placenta and umbilical cord tissues. In these tissues, the oligosaccharides were shown to react positively for a series of histochemical procedures for neutral complex carbohydrates such as periodic acid-Schiff (PAS), peroxidase-labelled Ricinus communis agglutinin-I-diaminobenzidine (PO-RCA-DAB) and concanavalin A-peroxidase-diaminobenzidine (Con A-PO-DAB). The asparagine-linked carbohydrates were localized in the placental villi, blood vessels and perivascular tissues and the umbilical cord blood vessels and matrix. The results of previous biochemical analyses performed upon the same tissues (Takahashi et al., 1981) have corroborated the results of the histochemical studies. The present results appear to substantiate the usefulness of almond glycopeptidase for the histochemical demonstration of the particular oligosaccharides of glycoproteins in tissues in general.
D015100:3,3'-Diaminobenzidine; D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000378:metabolism; D000581:Amidohydrolases; D003208:Concanavalin A; D005260:Female; D006023:Glycoproteins / Q000032:analysis* / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins; D009844:Oligosaccharides / Q000032:analysis* / Q000378:metabolism; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D010503:Periodic Acid-Schiff Reaction; D010544:Peroxidases; D010920:Placenta / Q000032:analysis* / Q000033:anatomy & histology; D037121:Plant Lectins; D011247:Pregnancy; D014470:Umbilical Cord / Q000032:analysis* / Q000033:anatomy & histology
D006023:Glycoproteins; D037102:Lectins; D009844:Oligosaccharides; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D003208:Concanavalin A; C000009:N-acetylglucosaminylasparagine; D015100:3,3'-Diaminobenzidine; D010544:Peroxidases; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D000117:Acetylglucosamine