A simple spectrophotometric method for the direct determination of uric acid in avian excreta.
A procedure was developed for the direct quantitation of uric acid in poultry excreta. It involved the determination of the absorbance of a perchloric acid extract of excreta at 285 nm. Interference from other ultraviolet absorbing material was shown to be negligible. The correlation (r) between this procedure and a recently developed high-performance liquid chromatographic procedure for uric acid content of excreta from birds fed different diets was .993 (P less than .01). It was concluded that this procedure provides a simple and reliable method for the quantitation of uric acid in avian excreta.
D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000032:analysis*; D005260:Female; D006863:Hydrogen-Ion Concentration; D008297:Male; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014527:Uric Acid / Q000032:analysis*
D014527:Uric Acid