title: Conversion of triostins to quinomycins by protoplasts of Streptomyces echinatus.
abstract: Protoplasts of Streptomyces echinatus have been used to investigate the biosynthesis of echinomycin (quinomycin A). It has been shown that this organism has the capacity to convert a series of triostins to the corresponding quinomycins by a mechanism involving methylation. Evidence is presented which suggests that triostin A is the natural precursor of echinomycin. Conversion of tetra-N-demethyl analogues of triostin A to corresponding analogues of echinomycin was not detected.
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000378:metabolism*; D002250:Carbon Radioisotopes; D004448:Echinomycin / Q000031:analogs & derivatives / Q000096:biosynthesis*; D007700:Kinetics; D011523:Protoplasts / Q000378:metabolism*; D011810:Quinoxalines / Q000096:biosynthesis* / Q000378:metabolism; D013302:Streptomyces / Q000378:metabolism*; D013462:Sulfur Radioisotopes
chemical_list: D000900:Anti-Bacterial Agents; D002250:Carbon Radioisotopes; D011810:Quinoxalines; D013462:Sulfur Radioisotopes; C009589:triostin C; C010777:quinomycin; C031945:triostin A; D004448:Echinomycin