Improved resolution of the 2-deoxy-D-glucose technique.
It was attempted to improve the resolution of the 2-deoxyglucose method. Two principle changes in the procedure were introduced: the gluing of the sections on to the object slide at--20 degrees C and the application of the emulsion with the loop technique. With this approach autoradiographs with grain accumulations over single cell bodies could be observed in many brain regions in addition to a diffuse activity over neuropil.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001786:Blood Glucose / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D001931:Brain Mapping; D003847:Deoxyglucose / Q000378:metabolism; D008297:Male; D009474:Neurons / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D001786:Blood Glucose; D003847:Deoxyglucose