title: Sterol-free eukaryotic cells from continuous cell lines of insects.
abstract: Three cell lines from Mamestra brassicae (Lepidoptera), one from Aedes albopictus (Diptera) and one from Sarcophaga peregrina (Diptera) were continuously cultured in a sterol-free medium and analyzed for sterol content. No sterols were found in any of the cell lines, whereas from cells cultured in a serum-containing medium, cholesterol was detected. This is the first instance of sterol-free animal cells.
mesh_terms: D000330:Aedes / Q000166:cytology; D000818:Animals; D002460:Cell Line; D002477:Cells / Q000378:metabolism*; D004175:Diptera / Q000166:cytology; D005057:Eukaryotic Cells / Q000378:metabolism*; D007313:Insecta / Q000166:cytology*; D007915:Lepidoptera / Q000166:cytology; D013261:Sterols / Q000032:analysis*
chemical_list: D013261:Sterols