title: The influence of choline chloride on murine hematopoiesis in vivo and in vitro.
abstract: We report here studies to further document the ability of cholinergic agonists to influence murine hematopoiesis. B6C3F1 mice were administered ultrapure choline (0.14, 1.4, and 7.0 mg/animal) i.p. for three days, then randomly sacrificed for assessment of hematological parameters. No significant change was observed in the packed red cell volume; however, the WBC was elevated significantly (85-400%) over noncholine administered controls during all days examined [1-12]. In vitro choline (10 mM) increased heterogeneous marrow-derived CFU-GM colony formation 210% above controls. Nonadherent marrow cell-derived CFU-GM produced a significant elevation in colony formation (36%), suggesting choline may influence directly a portion of the CFU-GM population. A twofold increase in the CFU-GM kill after 16 mM hydroxyurea exposure suggests choline can stimulate a subpopulation of CFU-GM not normally in cell cycle. In addition, choline (10 mM) was effective in stimulating CSF activity from marrow-derived conditioned medium. Dose-response studies demonstrated that choline was also stimulatory for megakaryocyte colony formation (CFU-Mk), 26% at 5 mM. Erythroid precursor stem cells, CFU-E/BFU-E, were reduced significantly (10-50%) at all choline concentrations tested (1-50 mM). These studies document the ability of choline to influence hematopoiesis, and provide further evidence that cholinergic mechanisms may be important in the control of steady-state hematopoiesis.
mesh_terms: D000818:Animals; D002794:Choline / Q000494:pharmacology*; D003114:Colony-Forming Units Assay; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006918:Hydroxyurea / Q000494:pharmacology; D066298:In Vitro Techniques; D051379:Mice
chemical_list: D002794:Choline; D006918:Hydroxyurea