Comparison of the sister chromatid exchange and cell survival assays as a measure of tumor cell sensitivity in vitro to cis-diamminedichloroplatinum (II).
Cytotoxic effects of treating RIF-1, EMT6, and 9L tumor cell lines with cis-diamminedichloroplatinum(II) were measured with the sister chromatid exchange assay and compared to results obtained with the colony-forming efficiency assay. The greatest number of sister chromatid exchanges was induced in RIF-1 cells, fewer in EMT6 cells, and the least in 9L cells. Cell survival data obtained with the colony-forming efficiency assay paralleled data obtained with the sister chromatid exchange assay. These studies suggest that the sister chromatid exchange assay may be a useful method with which to determine the in vitro sensitivity of tumor cells to some antineoplastic agents.
D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D003434:Crossing Over, Genetic / Q000187:drug effects*; D066298:In Vitro Techniques; D051381:Rats; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013997:Time Factors
D002945:Cisplatin