Molecular cloning of the rat metallothionein 1 (MT-1) mRNA sequence.
Starting with liver mRNA from cadmium chloride-treated rats, we constructed a cDNA library. A mouse metallothionein 1 (MT-1) cDNA restriction fragment (Durnam et al. 1980) was used as a hybridization probe to isolate from this library a cDNA (p2A10) which is a nearly complete copy of the rat MT-1 mRNA sequence. Southern analysis of rat DNA, using p2A10 as a probe, together with the screening of a rat genomic library, reveal that the rat MT-1 gene belongs to a family of related sequences. The rat MT-1 gene is inducible both by glucocorticoids and by metals.
D000818:Animals; D001483:Base Sequence; D002104:Cadmium / Q000494:pharmacology; D019256:Cadmium Chloride; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005796:Genes; D006854:Hydrocortisone / Q000494:pharmacology; D008099:Liver / Q000378:metabolism; D008667:Metalloproteins / Q000235:genetics*; D008668:Metallothionein / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats
D008667:Metalloproteins; D012333:RNA, Messenger; D002104:Cadmium; D004247:DNA; D008668:Metallothionein; D019256:Cadmium Chloride; D006854:Hydrocortisone