Interaction of N6-substituted-9-methyladenines to poly-5-bromouridylic acid.
Interaction of N6-methyl-9-methyladenine (m6m9A) to poly-5-bromouridylic acid (poly(BU] was investigated. The stoichiometry of interaction was determined to be 1 m6A:2 BU by equilibrium dialysis. Structural information was obtained from circular dichroism and absorption spectra and a similarity of the structure to the corresponding polymer complex was suggested. Thermodynamic parameters of the interaction was calculated from dependence of TmS on the monomer concentration and compared to those from calorimetric measurements. N6-(delta 2-Isopentenyl) - and N6-allyl-9-methyladenine were also examined of the binding to poly(BU).
D000225:Adenine / Q000031:analogs & derivatives*; D002942:Circular Dichroism; D006860:Hydrogen Bonding; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D011061:Poly A / Q000031:analogs & derivatives*; D011072:Poly U / Q000031:analogs & derivatives*; D013056:Spectrophotometry, Ultraviolet
D011061:Poly A; D011072:Poly U; C031089:poly-5-bromouridylic acid; C013115:9-methyladenine; D000225:Adenine