[Histone synthesis at S- and G2-phases of the mitotic cycle of human diploid fibroblasts].
The phase-specific synthesis of the total protein, histones, RNA and DNA in human embryonic fibroblasts synchronized by double thymidine blockade was investigated. It was shown that activation of histone synthesis occurs in three steps, i.e. i) immediately before replication, ii) after DNA synthesis maximum, and, iii) at the G2-phase of the mitotic cycle. During maximal replication of DNA histone synthesis is suppressed. It was assumed that at the pre-replication phase histone synthesis controls decondensation, while and the G2-phase--condensation of chromatin.
D002478:Cells, Cultured; D004261:DNA Replication; D004622:Embryo, Mammalian; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006657:Histones / Q000235:genetics*; D006801:Humans; D007399:Interphase; D007700:Kinetics; D011247:Pregnancy; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
D006657:Histones
Sintez gistonov v S- i G2-fazakh mitoticheskogo tsikla diploidnykh fibroblastov cheloveka.