Healing of biodegradable vascular prosthesis. Incorporation of 3H-valine into proteins in the subendothelial scar and host intima-media of rat aorta.
Heparin treated and aldehyde crosslinked rat aorta segments were implanted in infrarenal aorta of homologous rats. One year following aortic replacement, the subendothelial scar and the prosthetic remnants were excised. The scar and the host intima-media were incubated with 3H-valine for 4 h and extracted with 5 M guanidinium chloride--0.05 M dithiothreitol--0.1 M Tris--0.1% EDTANa2 at pH 7.5 prior (Extract 1) and following (Extract 2) hydrolysis of collagen. The radioactivity of extract 1 accounted for approximately 80% of the total label incorporated in the scar and host intima-media. The 3H-label of extract 1 adjusted for the tissue collagen content was about twenty times higher in the scar than in the host aorta. The major 3H protein peaks from Extract 1 of scar and host aorta were of 130 K, 100 K and 70 K apparent molecular weight, based on polyacrylamide gel electrophoresis in SDS. Hydrolysis with 2N KOH of the extraction residue from the host aorta and scar yielded 3H-val-pro dipeptides and hydrolysis with 6N HCl desmosines. The incorporation pattern of 3H-valine into proteins and the presence of elastin synthesized de novo in the scar replacing the prosthesis indicate macromolecular repair of the host aortic wall.
D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000637:transplantation*; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D002921:Cicatrix / Q000378:metabolism; D004549:Elastin / Q000096:biosynthesis; D004727:Endothelium / Q000378:metabolism; D006868:Hydrolysis; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014184:Transplantation, Homologous; D014633:Valine / Q000378:metabolism*
D011506:Proteins; D004549:Elastin; D014633:Valine