Radioimmunoassay of a glycoprotein associated with malignancy.
A glycoprotein associated with malignancy was purified from the 0.6M perchloric acid-soluble fraction of serum obtained from cancer patients. The purified glycoprotein contained sialic acid, which was responsible for binding to wheat-germ agglutinin-Sepharose. Gel electrophoresis showed one band with an apparent Mr of 50 000-55 000, and the isoelectric point was 4.4 +/- 0.1. The glycoprotein could be distinguished from carcinoembryonic antigen and alpha-fetoprotein. Iodination of this material with chloramine-T permitted development of a radioimmunoassay.
D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D007122:Immunoelectrophoresis; D008970:Molecular Weight; D009369:Neoplasms / Q000097:blood*; D010956:Plasmapheresis; D011863:Radioimmunoassay / Q000379:methods
D006023:Glycoproteins