Simple preparation of 1,2-dipalmitoyl-sn-glycero-3-phosphoric acid and deuterated choline derivatives.
Analytically pure 1,2-dipalmitoyl-sn-glycero-3-phosphoric acid was prepared in gram amounts, using a simplified version of a previous procedure. The main step, enzymatic cleavage of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine with freshly extracted phospholipase D, was performed in the presence of chloroform and the crude phosphatidic acid was purified by silica gel column chromatography. The interest of the method was illustrated by the synthesis of two dipalmitoyl phosphatidylcholines selectively deuterated on the polar headgroup.
D003903:Deuterium; D010712:Phosphatidic Acids / Q000138:chemical synthesis*; D010739:Phospholipase D / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D011663:Pulmonary Surfactants
D010712:Phosphatidic Acids; D011663:Pulmonary Surfactants; C031599:dipalmitoylphosphatidic acid; D003903:Deuterium; D010740:Phospholipases; D010739:Phospholipase D