Different uptake in rat peritoneal macrophages of beta-very low density lipoprotein prepared from Sprague-Dawley rats and hypercholesterolemic rats (ExHC rats).
Rat beta-migrating very low density lipoprotein (beta-VLDL) was obtained from both ExHC rats and Sprague-Dawley rats which had been fed a cholesterol diet for 4 days, and was incubated with peritoneal macrophages from both kinds of rat to compare the cellular uptake of these lipoproteins. ExHC rat beta-VLDL was taken up by the cells three to five times as well as Sprague-Dawley rat beta-VLDL. Uptake depended solely on the lipoproteins used and not on the cells. Both lipoproteins seemed to be slightly different from each other in apolipoprotein composition. This stimulated uptake may explain the aortic lipid deposits found in ExHC rats with dietary hypercholesterolemia.
D000818:Animals; D001057:Apolipoproteins E / Q000378:metabolism; D001202:Ascitic Fluid / Q000378:metabolism; D002788:Cholesterol Esters / Q000096:biosynthesis; D006937:Hypercholesterolemia / Q000378:metabolism*; D066298:In Vitro Techniques; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011973:Receptors, LDL / Q000378:metabolism
D001057:Apolipoproteins E; D002788:Cholesterol Esters; D008079:Lipoproteins, VLDL; D011973:Receptors, LDL; C036053:VLDL receptor