title: Effects of amyotrophic lateral sclerosis sera on cultured cholinergic neurons.
abstract: Dissociated monolayer cultures of chick ciliary ganglion neurons have been used to study the effects of control and ALS sera. The cultured neurons survive and extend neurites for a minimum of 2 weeks in a standard tissue culture medium that contains 10% heat-inactivated human serum. Three parameters of the neurons have been examined when cultured in control and ALS sera for 8 to 12 days: (1) neuronal survival, (2) activity of the enzyme choline acetyltransferase, and (3) synthesis of 3H-acetylcholine using 3H-choline as precursor. ALS sera cause a small decrease in these three parameters, but this difference is not significant.
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000328:Adult; D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000097:blood / Q000378:metabolism*; D000818:Animals; D002452:Cell Count; D002642:Chick Embryo; D002794:Choline / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003470:Culture Media; D005260:Female; D005726:Ganglia, Parasympathetic / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000378:metabolism; D014316:Tritium
chemical_list: D003470:Culture Media; D014316:Tritium; D002795:Choline O-Acetyltransferase; D002794:Choline; D000109:Acetylcholine