The radiosensitivity of lymphosarcoma cells as determined by the liver colony method.
The liver colony method is based on the observation that intravenous injection of an appropriate number of LS/BL cells into isologous non-irradiated hosts leads to the formation of colonies of proliferating cells in the livers of these animals. The relationship between the number of cells injected and the number of colonies appearing in the livers was determined. This technique was used to measure the radiation sensitivity of LS/BL cells and it yielded a Do of 1.05 Gy. The results show that LS/BL cells have a similar radiation sensitivity as other mammalian cells. The liver colony assay used to determine the radiation sensitivity of the lymphosarcoma LS/BL cells can also be used whenever the number of viable tumour cells in a suspension is to be estimated.
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects; D003114:Colony-Forming Units Assay; D005260:Female; D008099:Liver / Q000473:pathology*; D008228:Lymphoma, Non-Hodgkin / Q000532:radiotherapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis; D013154:Spleen / Q000473:pathology