Autoradiographic demonstration of the penetration of albumen-derived material through the vitelline membrane into the egg yolk, exterior to the avian blastoderm.
A method enabling in ovo transplantation and cultivation of fertilized, unincubated quail yolks is described. By this technique, nonradioactive yolks were transferred into nonradioactive egg shells filled with in vivo radioactively labeled albumen. With autoradiography it was demonstrated that during early avian blastoderm development, albumen-derived material can penetrate through the vitelline membrane of the acellular rimzone distal to the margin of over-growth.
D000818:Animals; D001345:Autoradiography; D001756:Blastoderm / Q000637:transplantation*; D003370:Coturnix / Q000196:embryology*; D004530:Egg Yolk; D004625:Embryo, Nonmammalian / Q000637:transplantation*; D005260:Female; D010047:Ovalbumin / Q000378:metabolism*; D011784:Quail / Q000196:embryology*; D014817:Vitelline Membrane / Q000378:metabolism*
D010047:Ovalbumin