Effect of cyclophosphamide on specific IgM and IgG responses and recovery during the various stages of persistent immunization in the Brucella-rabbit model.
The effect of one or several doses of cyclophosphamide (CP) administered to rabbits persistently immunized with Brucella abortus was investigated. The goal was to halt the response at different stages of the hyperimmunization process and to study the kinetics and IgM- or IgG-nature of the response following CP discontinuation. It was observed that CP halted the response; and once the drug had been discontinued, the post-CP response reached titres much higher than those of either the controls or the animals before CP administration (rebound effect). On the other hand, it was noticed that the post-CP response was mainly IgM when the drug was given in the early stages of hyperimmunization, and IgG when the drug was given at later stages.
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000917:Antibody Formation / Q000187:drug effects; D002004:Brucella Vaccine / Q000008:administration & dosage / Q000276:immunology; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000494:pharmacology*; D004306:Dose-Response Relationship, Immunologic; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D007700:Kinetics; D008954:Models, Biological; D011817:Rabbits
D000907:Antibodies, Bacterial; D002004:Brucella Vaccine; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D003520:Cyclophosphamide