Autoradiographic localization of binding sites for the delta sleep-inducing peptide ( [3H]DSIP) on neurons of cultured rat brainstem.
Binding of the delta sleep-inducing peptide (DSIP) was studied in cultures of rat brainstem by means of autoradiography. Binding sites for [3H]DSIP were observed on small, medium-sized and large brainstem neurons but not on glial cells. Addition of unlabeled DSIP inhibited or markedly reduced binding of [3H]DSIP. It is suggested that brainstem neurons might possess receptors for this sleep-inducing peptide.
D000818:Animals; D001345:Autoradiography; D001933:Brain Stem / Q000378:metabolism*; D046508:Culture Techniques; D003701:Delta Sleep-Inducing Peptide; D007700:Kinetics; D008526:Medulla Oblongata / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism*; D011149:Pons / Q000378:metabolism; D051381:Rats
D009842:Oligopeptides; D003701:Delta Sleep-Inducing Peptide