Mutagenic and DNA damaging activity in muscle of trout exposed in vivo to nitrite.
Muscle ether extracts of rainbow trout (Salmo gairdneri) exposed to lake water enriched with nitrite (450 micrograms/l) reverted plasmid-containing his- strains of Salmonella typhimurium, mainly eliciting frameshift mutations, and induced a DNA damage in Escherichia coli reparable through the recA/lexA-dependent SOS functions. The number of revertants was related to their content in nitroso-derivatives and to the physiological condition of the fish. Mutagenicity was efficiently decreased, through NADPH-requiring pathways, by liver S-9 fractions from rats or rainbow trout, while it was not affected by preliminary heating nor by pre-incubation with human gastric juice.
D000818:Animals; D004247:DNA / Q000378:metabolism*; D005618:Fresh Water; D009132:Muscles / Q000378:metabolism*; D009152:Mutagenicity Tests; D009153:Mutagens; D009573:Nitrites / Q000633:toxicity*; D012977:Sodium Nitrite / Q000633:toxicity*; D014020:Tissue Extracts / Q000633:toxicity; D014337:Trout; D014873:Water Pollutants / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D009153:Mutagens; D009573:Nitrites; D014020:Tissue Extracts; D014873:Water Pollutants; D014874:Water Pollutants, Chemical; D004247:DNA; D012977:Sodium Nitrite