Determination of pipecolic acid in rat brain areas by high-performance liquid chromatography of dansyl derivatives with fluorimetric detection.
A method for the determination of pipecolic acid in the rat brain is reported. The identification and quantification of pipecolic acid is accomplished with reverse-phase high-performance liquid chromatography including precolumn dansylation procedure by using nipecotic acid, a regio-isomer of pipecolic acid, as an internal standard. The lower limit of quantification for the method is in the picomole range. Higher concentration of pipecolic acid in the rat brain regions were found in cerebellum, medulla oblongata, hypothalamus, and midbrain than the other regions. The method establishes the usefulness of dansyl chloride for the simple and sensitive detection of pipecolic acid, and is easily adapted to routine analysis of pipecolic acid in the rat brain regions.
D000818:Animals; D001923:Brain Chemistry; D002531:Cerebellum / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003619:Dansyl Compounds; D005470:Fluorometry; D007031:Hypothalamus / Q000032:analysis; D008297:Male; D008526:Medulla Oblongata / Q000032:analysis; D010875:Pipecolic Acids / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains
D003619:Dansyl Compounds; D010875:Pipecolic Acids