Recovery of proteins on a milligram scale from polyacrylamide electrophoresis gels, exemplified by purification of a retinol-binding protein.
An apparatus suitable for the recovery of proteins from polyacrylamide gels on a milligram scale by displacement electrophoresis (isotachophoresis) is described along with a buffer system that is suitable for this purpose with most proteins. The technique is illustrated by the recovery of a protein from a 15% polyacrylamide gel. The recovery was almost quantitative and the eluted protein showed little contamination upon quantitative amino acid analysis and automatic Edman degradation.
D000595:Amino Acid Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D004586:Electrophoresis / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D011506:Proteins / Q000302:isolation & purification*; D012177:Retinol-Binding Proteins / Q000302:isolation & purification*
D011506:Proteins; D012177:Retinol-Binding Proteins