Differences in the rates of aggregation of proteoglycans from human articular cartilage and chondrosarcoma.
Pieces of adult human articular cartilage and chondrosarcoma were incubated in the presence of [35S]sulphate. After continuous or pulse-change incorporation of radioactivity, proteoglycans were extracted with 4.0 M-guanidinium chloride, purified by equilibrium density-gradient centrifugation and fractionated by gel chromatography. A comparison of the results suggests that the formation of stable aggregates occurs at a lower rate in articular cartilage than in chondrosarcoma.
D000328:Adult; D000368:Aged; D002358:Cartilage, Articular / Q000378:metabolism*; D002813:Chondrosarcoma / Q000378:metabolism*; D002850:Chromatography, Gel; D046508:Culture Techniques; D006801:Humans; D046911:Macromolecular Substances; D011509:Proteoglycans / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism
D046911:Macromolecular Substances; D011509:Proteoglycans; D013431:Sulfates