The effects of membrane modification and hyperthermia on the survival of P-388 and V-79 cells.
Cells with greater membrane microviscosities are reportedly less sensitive to being killed by mild hyperthermia. To further study this phenomenon, membrane microviscosity of ascites P-388 tumor cells was increased by adding cholesteryl hemisuccinate (CHS). Unexpectedly, when modified cells were heated for 60 min at 43 degrees C in vitro and analyzed in vivo in CDF1 mice they showed an increased thermal sensitivity. Similar increases in cell membrane microviscosity were obtained with V-79 cells. However, after heat treatment no differences in survival in vitro were noted between modified and unmodified cells. Treatment with CHS alone results in a substantial increase in P-388 cells, which take up trypan blue, but not in V-79 cells. When these 'dead' cells are accounted for, the difference in killing between control and CHS-modified P-388 cells is no longer seen. When considered in this light, both P-388 and V-79 cells are similar in their response to heat, which is not influenced by CHS per se.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects*; D002470:Cell Survival; D002784:Cholesterol / Q000378:metabolism; D002788:Cholesterol Esters / Q000494:pharmacology*; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D006358:Hot Temperature; D007941:Leukemia P388 / Q000473:pathology; D051379:Mice; D014783:Viscosity
D002788:Cholesterol Esters; D002784:Cholesterol; C013440:cholesteryl succinate