p-Nitrophenyl alpha-D-glucopyranoside, a new substrate for glucansucrases.
p-Nitrophenyl alpha-D-glucopyranoside has been shown to be a substrate for the glucansucrases of various strains of Leuconostoc mesenteroides and Streptococcus mutans. The products from a digest of p-nitrophenyl alpha-D-glucopyranoside with L. mesenteroides B-512F dextransucrase were found to include dextran, a series of p-nitrophenyl isomaltodextrin glycosides, and p-nitrophenyl nigeroside. The kinetics of the reaction were non-Michaelis-Menten, possibly because p-nitrophenyl alpha-D-glucopyranoside has a dual role in the reaction as both a D-glucosyl donor and acceptor.
D002855:Chromatography, Thin Layer; D005960:Glucosides / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006027:Glycosides / Q000378:metabolism*; D016695:Glycosyltransferases; D007700:Kinetics; D007934:Leuconostoc / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D013295:Streptococcus mutans / Q000201:enzymology*
D005960:Glucosides; D006027:Glycosides; C019502:4-nitrophenyl alpha-glucoside; D016695:Glycosyltransferases; C033837:alternansucrase; D006026:Glycoside Hydrolases