title: Characteristics of the cytoplasmic and nuclear dihydrotestosterone receptors of human thymic tissue.
abstract: Dihydrotestosterone receptor was prepared from both the cytoplasm and nucleus of human thymic tissue. Human cytoplasmic and nuclear dihydrotestosterone receptor possessed a KA greater than 1.0 and by competition assay was highly specific for dihydrotestosterone. Human thymic cytoplasmic dihydrotestosterone receptor prepared in standard buffer sedimented on 5-20% sucrose gradients in the 4s region but human thymic cytoplasmic dihydrotestosterone receptor prepared in buffer containing sodium molybdate (10 mM) sedimented in the 7s region on 5-20% sucrose gradients. It is concluded that the human thymic dihydrotestosterone receptor is similar to classic androgen receptor.
mesh_terms: D000293:Adolescent; D000328:Adult; D002467:Cell Nucleus / Q000378:metabolism*; D002499:Centrifugation, Density Gradient / Q000379:methods; D002648:Child; D002675:Child, Preschool; D003600:Cytosol / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism*; D005260:Female; D006801:Humans; D007223:Infant; D007700:Kinetics; D008297:Male; D011944:Receptors, Androgen / Q000302:isolation & purification / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D012737:Sex Factors; D013950:Thymus Gland / Q000378:metabolism*
chemical_list: D011944:Receptors, Androgen; D011987:Receptors, Steroid; D013196:Dihydrotestosterone