Post-translational modification of tubulin in cultured neurons.
Isoelectricfocusing x SDS polyacrylamide gel electrophoresis of cultured sympathetic neurons revealed two alpha- and two beta-subunits of tubulin. In pulse-chase experiments, the spot morphology of the beta-subunits synthesized during a 10 min pulse did not change appreciably during the chase period. However, the appearance of the labeled alpha-subunits changed during the chase period. With chase periods of less than 4 h, the labeled alpha-subunits focused as discrete spots, while after chase periods of greater than 6 h, they focused as short streaks.
D000818:Animals; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D009474:Neurons; D014176:Protein Biosynthesis; D051381:Rats; D013564:Sympathetic Nervous System / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism*
D014404:Tubulin