Covalent binding of 6-nitrobenzo-[a]pyrene metabolites to DNA in vitro.
Incubation of 6-nitrobenzo[a]pyrene[7, 10-14C] and calf thymus DNA with rat liver S9 fractions resulted in covalent binding of nitrobenzo[a]pyrene to DNA. In the presence of flavin mononucleotide under anaerobic conditions, the amount of binding decreased. Binding studies using synthetic polynucleotides showed that there was a high preference for poly(dG) compared to poly(dA), poly(dC) and poly(T).
D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism*; D002850:Chromatography, Gel; D004247:DNA / Q000378:metabolism*; D005486:Flavin Mononucleotide / Q000494:pharmacology; D008099:Liver / Q000378:metabolism; D008297:Male; D009153:Mutagens / Q000378:metabolism*; D009249:NADP / Q000494:pharmacology; D051381:Rats; D014239:Trichloroepoxypropane / Q000494:pharmacology
D001580:Benzopyrenes; D009153:Mutagens; D014239:Trichloroepoxypropane; D009249:NADP; C034699:6-nitrobenzo(a)pyrene; D005486:Flavin Mononucleotide; D004247:DNA