A polarographic method for the simultaneous determination of total glucosinolates and free glucose of cruciferous material.
A rapid method for the simultaneous analysis of total free glucose and total glucosinolates in aqueous extracts of cruciferous material is described. The technique, which appears suitable for plant-breeding programs as it allows the processing of more than 100 samples per day, involves the polarographic determination of O2 uptake of free glucose by a system of double-coupled enzymes, such as myrosinase-glucose oxidase. The method has advantages over current methods, because it is very rapid (4 min per analysis), allows two determinations for each analysis, and appears to be very reproducible, accurate, and sensitive.
D001937:Brassica / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D005947:Glucose / Q000032:analysis*; D005961:Glucosinolates / Q000032:analysis*; D006026:Glycoside Hydrolases; D010936:Plant Extracts / Q000032:analysis*; D011048:Polarography; D013865:Thioglycosides / Q000032:analysis*
D005961:Glucosinolates; D010936:Plant Extracts; D013865:Thioglycosides; D006026:Glycoside Hydrolases; C022739:thioglucosidase; D005947:Glucose