Interferon as an in vitro modifier of immune responses induced by combined vaccine (DTP) in mice.
The ability of DTP (diphtheria, tetanus, pertussis) vaccine to stimulate IgG-plaque forming cells (PFC) in mice was markedly inhibited by human leukocyte and mouse fibroblast interferon (IF). This effect of IF was not dose-dependent. The maximum inhibitory effect of human IF was observed on Day 10 after the administration of the vaccine. The inhibition of IgM-PFC was more severe when human IF was used. Although IF abrogated the effect of DTP vaccine on IgG-PFC, at the same time it stimulated PFC in the control group of non-vaccinated animals. The administration of DTP vaccine decreased IgM-PFC on Day 5, the effect was enforced by the addition of both human and mouse IF. IF decreased IgM-PFC in the control group. Small and large doses of DTP suppressed the leukocyte adherance inhibition (LAI) on Day 5 after administration of the vaccine, the effect was not affected by addition of IF. The suppressive effect of DTP was lost on Day 10 when addition of IF increased the percent of LAI in all experimental groups. Thus IF appears to interfere with the effect of DTP and selectively modulate the different immune responses.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D000921:Antibody-Producing Cells / Q000187:drug effects*; D004168:Diphtheria Toxoid / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007370:Interferon Type I / Q000494:pharmacology*; D007957:Leukocyte Adherence Inhibition Test; D051379:Mice; D009929:Organ Size; D010567:Pertussis Vaccine / Q000276:immunology*; D013154:Spleen / Q000033:anatomy & histology; D013745:Tetanus Toxoid / Q000276:immunology*
D004168:Diphtheria Toxoid; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007370:Interferon Type I; D010567:Pertussis Vaccine; D013745:Tetanus Toxoid