Identification and isolation of transcribed human X chromosome DNA sequences.
A human X chromosome specific phage library has been used as a source of X-specific genomic DNA clones which hybridize with cellular RNA. Random cDNA clones were mapped for X chromosome sequence localization and 8 were identified as hybridizing to X chromosome Hind III fragments. All eight also hybridized with autosomal Hind III fragments. The X chromosome genomic sequences corresponding to two of these cDNA clones were isolated from a phage library constructed with the Hind III endonuclease digest products of X enriched DNA. One genomic DNA segment, localized to the short area of the X, shared sequence homology with at least one region of the human Y chromosome. The methodology developed represents a rapid means to obtain a specific genomic DNA clone from a single chromosome when multiple different genomic loci homologous to an expressed DNA sequence exist.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D005260:Female; D006367:HeLa Cells / Q000502:physiology; D006801:Humans; D006822:Hybrid Cells / Q000502:physiology; D008297:Male; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014158:Transcription, Genetic; D014960:X Chromosome / Q000502:physiology*; D014998:Y Chromosome / Q000502:physiology
D004247:DNA