title: Cellular location of N-acetyltransfer activities toward glucosamine and glucosamine-6-phosphate in cultured human skin fibroblasts.
abstract: The intracellular location in normal human cultured skin fibroblasts of the N-acetyltransferase activities that transfer the acetyl group from acetyl-CoA to the 2-amino group of glucosamine and glucosamine-6-phosphate have been investigated. Organelles have been separated using a combination of differential centrifugation and free flow electrophoresis. The intracellular distribution of the enzyme involved in the N-acetyltransfer to glucosamine and an alpha-glucosaminide disaccharide indicated that this enzyme activity concentrates mainly with lysosomal organelles whereas the activity associated with N-acetyltransferase to glucosamine-6-phosphate is non-lysosomal. It is proposed that acetyl-CoA: alpha-glucosaminide N-acetyltransferase may be used as a convenient enzyme marker of lysosomal organelle membranes.
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism*; D005347:Fibroblasts / Q000201:enzymology*; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000378:metabolism*; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives*; D005958:Glucosephosphates / Q000378:metabolism*; D006801:Humans; D008247:Lysosomes / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D005958:Glucosephosphates; C001293:glucosamine 6-phosphate; D019298:Glucose-6-Phosphate; D000123:Acetyltransferases; C020580:glucosamine acetyltransferase; D005944:Glucosamine