Pyruvate kinase isoenzymes in human serum.
Pyruvate kinase activity in serum from presumably healthy individuals is labile, but can be maintained for a week when samples are stored frozen. Contrary to published data our electrophoretic and isoelectrofocusing studies with fresh sera show the presence of both K and M isoenzymes; the latter predominates. In freezer-stored serum the putative M isoenzyme showed a progressive decrease in pl value as well as a decreased electrophoretic mobility.
D000328:Adult; D004588:Electrophoresis, Cellulose Acetate; D005615:Freezing; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000097:blood* / Q000302:isolation & purification; D008875:Middle Aged; D011770:Pyruvate Kinase / Q000097:blood* / Q000302:isolation & purification; D013048:Specimen Handling
D007527:Isoenzymes; D011770:Pyruvate Kinase