Fucosyltransferase activities in human lymphocytes and granulocytes. Blood group H-gene-specified alpha-2-L-fucosyltransferase is a discriminatory marker of peripheral blood lymphocytes.
Human lymphocytes and granulocytes were examined for blood group H-gene-specified alpha-2-L-fucosyl-transferase, alpha-3-L-fucosyltransferase and blood group Le-gene-specified alpha-4-L-fucosyltransferase. No alpha-4-L-fucosyltransferase was detectable in either cell type. alpha-3-L-Fucosyltransferase was readily demonstrable in both lymphocytes and granulocytes and the levels of activity per 10(6) cells were approximately the same. In contrast, alpha-2-L-fucosyltransferase was detectable in lymphocytes but not in similar numbers of granulocytes. The absence of alpha-2-L-fucosyltransferase was confirmed in granulocytes isolated from the blood of eighty unselected donors and was independent of ABO blood group or ABH secretor status.
D000017:ABO Blood-Group System; D005647:Fucosyltransferases / Q000097:blood / Q000235:genetics*; D006098:Granulocytes / Q000201:enzymology*; D006602:Hexosyltransferases / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology*; D000097631:Galactoside 2-alpha-L-fucosyltransferase
D000017:ABO Blood-Group System; D005647:Fucosyltransferases; D006602:Hexosyltransferases; D000097631:Galactoside 2-alpha-L-fucosyltransferase; C031692:galactoside 3-fucosyltransferase; C019818:3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase