Cross-linking of tobacco mosaic virus RNA and capped polyribonucleotides to 18S rRNA in wheat germ ribosome-mRNA complexes.
Tobacco mosaic virus RNA, forming 40S or 80S initiation complexes with wheat germ ribosomes, was covalently bound to 18S ribosomal RNA by the photoreaction with an RNA cross-linking agent, 4'-aminomethyl-4,5',8-trimethylpsoralen (AMT). Synthetic polyribonucleotide, poly(A, U), with the cap structure m7GpppGmC at the 5'-terminal was also cross-linked to 18S ribosomal RNA in 40S or 80S complexes with ribosomes by the AMT photoreaction. Polyuridylic acid with the same 5'-cap structure, forming 40S complexes but not 80S complexes with ribosomes, was most efficiently cross-linked to 18S ribosomal RNA by the psoralen photoreaction. These results suggest that the interactions between mRNA and 18S rRNA are not necessarily of strict complementarity but occur during formation of the complexes in eukaryotes. The 40S complexes would be then converted to 80S complexes in the presence of the AUG initiation codon or AUG-like triplets containing A and U on the polyribonucleotide chains which interact with 18S ribosomal RNA.
D003432:Cross-Linking Reagents / Q000494:pharmacology*; D011564:Furocoumarins / Q000494:pharmacology*; D010777:Photochemistry; D010944:Plants / Q000235:genetics*; D012315:RNA Caps / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014307:Trioxsalen / Q000031:analogs & derivatives / Q000494:pharmacology*; D014908:Triticum / Q000235:genetics
D003432:Cross-Linking Reagents; D011564:Furocoumarins; D012315:RNA Caps; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012367:RNA, Viral; C018822:aminomethyltrioxsalen; D014307:Trioxsalen