title: Effect of rise in plasma osmotic pressure on the milk-ejection reflex in the rat.
abstract: The effect of an increase in plasma osmolality on the milk-ejection reflex of rats was studied. The lactating rats, at day 8-12 of lactation, were anesthetized with urethane (1.1 g/kg, i.p.) and 8-11 pups which had been separated from their mother 16-18 h were put to the nipples to suckle. In 21 of 47 rats studied an intermittent pattern of milk ejection was recorded with a latency of 15-74 min (group-I rats). The mean interval between recurring milk ejections was 8.3 +/- 0.6 (S.E.M.) min and the mean amount of oxytocin released at each milk ejection estimated in 11 of them was 0.26 +/- 0.04 mU. The remaining 26 rats showed no milk ejection throughout the nursing period of more than 90 min (group-II rats). The intraperitoneal injection of 1.5 M NaCl (1 ml) had no effect on the interval between milk ejections but increased the amount of oxytocin released at each milk ejection by 2.4 times in group-I rats, as the plasma osmolality changed from 297.9 +/- 2.7 mOsm/kg to 310.4 +/- 3.6 mOsm/kg. On the other hand, the 1.5 M NaCl injection induced recurring reflex milk-ejections in all of the group-II rats, while the plasma osmolality increased from 307 +/- 2.8 mOsm/kg to 320 +/- 3.1 mOsm/kg. The mean interval was not significantly different from that observed in the group-I rats. The sensitivity of the mammary gland to oxytocin was not altered by the 1.5 M NaCl injection. The injection of isotonic NaCl had no effect on the milk-ejection reflex.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D001769:Blood; D005260:Female; D007774:Lactation / Q000187:drug effects; D008321:Mammary Glands, Animal / Q000502:physiology; D009994:Osmolar Concentration; D010121:Oxytocin / Q000494:pharmacology; D011247:Pregnancy; D011312:Pressure; D051381:Rats; D011919:Rats, Inbred Strains; D011930:Reaction Time / Q000502:physiology; D012018:Reflex / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D012965:Sodium Chloride; D010121:Oxytocin