[Measurement of the absolute cell count in the heart and liver. The quantitative preservation of proteins and DNA in isolated cells].
Some improvement has been made of the Belov et al. (1975) method of alkaline dissociation of tissues. No cell loss occurs during cell suspension preparation or other procedures. The improved method was shown to be suited for determination of absolute numbers of cardiomyocytes and hepatocytes. Comparison of the living and dissociated hepatocytes revealed preservation of the total cell dry weight and DNA content (classes of ploidy) in the cells treated with formol and concentrated alkali. The method is recommended for studying the cell number, weight, ploidy and mitoses, and for DNA autoradiography.
D000818:Animals; D002452:Cell Count / Q000379:methods; D002469:Cell Separation; D004247:DNA / Q000032:analysis*; D006651:Histocytochemistry; D008099:Liver / Q000166:cytology*; D051379:Mice; D009206:Myocardium / Q000166:cytology*; D011506:Proteins / Q000032:analysis*; D051381:Rats; D013535:Suspensions
D011506:Proteins; D013535:Suspensions; D004247:DNA
Izmerenie absoliutnogo chisla kletok v serdtse i pecheni. Kolichestvennoe sokhranenie belkov i DNK v izolirovannykh kletkakh.