Evidence for a spectrin-like protein as a major component of the synaptosomal membrane cytoskeleton.
After treatment of synaptosomes with Nonidet-containing buffers, a proportion of the proteins remained insoluble. The major component (50%) of the residue was identified as a spectrin-like protein by immunodetection after mono- and bi-dimensional gel electrophoresis and transfer to nitrocellulose paper. Actin was also present.
D000818:Animals; D003599:Cytoskeleton / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D008297:Male; D008970:Molecular Weight; D051381:Rats; D013049:Spectrin / Q000032:analysis*; D013570:Synaptic Membranes / Q000648:ultrastructure*
D013049:Spectrin