Biotinated probe containing a long-terminal repeat hybridized to a mouse colon tumor and normal tissue.
The cloned complementary DNA pMCT-1, which contains an intracisternal A particle long-terminal repeat, is more highly expressed in a mouse colon tumor than in the normal mouse colon. In situ hybridization of biotin-substituted pMCT-1 to fixed frozen sections shows that expression of pMCT-1 is seen throughout the tumor and is highly heterogeneous on a cellular basis, while expression is undetectable in any cell in the normal colonic mucosa.
D000818:Animals; D001710:Biotin; D003106:Colon / Q000032:analysis*; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D004247:DNA; D007399:Interphase; D007413:Intestinal Mucosa / Q000032:analysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D012334:RNA, Neoplasm / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D012334:RNA, Neoplasm; D001710:Biotin; D004247:DNA