Paradoxical stimulation by amino acids of the degradation of [35S]methionine-labelled, short-lived protein in isolated rat hepatocytes.
A complete amino acid mixture inhibited the degradation of long-lived and [14C]valine-labelled short-lived protein in isolated rat hepatocytes, but paradoxically stimulated the degradation of [35S]methionine-labelled short-lived protein. The stimulation persisted in the presence of autophagiclysosomal pathway inhibitors like 3-methyladenine and propylamine, indicating the existence of an hitherto unrecognized non-lysosomal degradation mechanism with selectivity towards methionine-rich proteins or peptide regions.
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D008715:Methionine / Q000378:metabolism*; D011437:Propylamines / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism*; D051381:Rats
D000596:Amino Acids; D011437:Propylamines; D011506:Proteins; C025946:3-methyladenine; D008715:Methionine; D000225:Adenine; C035263:2-propylamine