Characterization of the exposed carbohydrates on the sheath surface of in vitro-derived Brugia pahangi microfilariae by analysis of lectin binding.
Adult female Brugia pahangi were maintained metabolically active in vitro for up to 35 days in Click's medium supplemented with 10% horse serum. For the first 14 to 18 days microfilariae were released into culture. Although these in vitro-derived microfilariae were morphologically identical to in vivo-derived microfilariae, they could be differentiated by their characteristic of binding to a panel of fluorescein-conjugated lectins. The results suggest that maturation and release of microfilariae are correlated with glycosidic alterations on the sheath surface.
D000818:Animals; D002009:Brugia / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D003470:Culture Media; D005260:Female; D005370:Filarioidea / Q000032:analysis*; D019793:Fluorescein; D005452:Fluoresceins; D037102:Lectins; D008842:Microfilariae / Q000032:analysis*
D002241:Carbohydrates; D003470:Culture Media; D005452:Fluoresceins; D037102:Lectins; D019793:Fluorescein