Opioid peptide-like immunoreactivity localized in GABAErgic neurons of rat neostriatum and central amygdaloid nucleus.
Opioid peptide-like (OPL)-immunoreactivity and (the GABA-biosynthetic enzyme) glutamic acid decarboxylase-like (GAD)-immunoreactivity were localized in rat neostriatum and central amygdaloid nucleus (ACE) using a polyclonal sheep antiserum to rat brain GAD and a monoclonal mouse antibody to the N-terminus of beta-endorphin (3-E7) as primary antisera. PAP-immunohistochemistry revealed GAD-immunoreactivity in the majority of neurons in neostriatum and ACE. OPL-immunoreactivity was observed in numerous neurons in ACE, but only in few neostriatal nerve cells. In double immunofluorescence in the same section OPL- and GAD-immunoreactivity colocalized in few medium size cells in the neostriatum, but in numerous neurons in ACE. The existence of opioid peptide containing GABAergic neurons in ACE and neostriatum is demonstrated.
D000679:Amygdala / Q000032:analysis* / Q000166:cytology; D000818:Animals; D003342:Corpus Striatum / Q000032:analysis* / Q000166:cytology; D004723:Endorphins / Q000032:analysis*; D007118:Immunoassay; D009474:Neurons / Q000032:analysis* / Q000166:cytology; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D004723:Endorphins; D005680:gamma-Aminobutyric Acid