Determination of catecholamines in urine by ion-exchange liquid chromatography with electrochemical detection.
A liquid chromatographic method for the determination of free urinary concentrations of epinephrine, norepinephrine and dopamine is presented. For urine samples, pre-purified by adsorption onto alumina, ion-exchange chromatography was, in terms of selectivity, found to be superior to the more widely used reversed-phase chromatography. The column eluates were monitored with an electrochemical detector utilizing a glassy carbon working electrode. The method allows determination of the concentrations in 0.5 ml of normal urine samples with a relative standard deviation below 2%.
D002395:Catecholamines / Q000652:urine*; D002852:Chromatography, Ion Exchange / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D004298:Dopamine / Q000652:urine; D004563:Electrochemistry; D004837:Epinephrine / Q000652:urine; D006801:Humans; D009638:Norepinephrine / Q000652:urine
D002395:Catecholamines; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine