Identification of two cDNA clones coding for androgen-dependent polypeptides in rat ventral prostate.
cDNA clones were obtained by transformation of E. coli chi 1776 with pBR322 containing insert of ds cDNA synthesized from total rat prostate poly(A)RNA. Two prostate-specific cDNA clones were isolated by colony hybridization and identified by message selection/translation as encoding polypeptides of Mr: 13,500 and 9,300. Hybridization of poly(A)RNA from normal and castrated rat prostates to the cloned cDNAs indicated that the levels of mRNAs coding for Mr: 13,500 and 9,300 polypeptides are regulated by testosterone.
D000728:Androgens / Q000378:metabolism*; D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D008297:Male; D009693:Nucleic Acid Hybridization; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics*; D011467:Prostate / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000728:Androgens; D010455:Peptides; D004247:DNA