Cell-free synthesis of the depsipeptide beauvericin.
The enzymatic formation of the cyclodepsipeptide beauvericin was demonstrated in cell-free extracts from Beauveria bassiana. In analogy to the enniatin synthetase system formation of beauvericin is strictly dependent on the presence of the constituent amino and hydroxy acid, S-adenosylmethionine, and ATP/Mg2+. Synthesizing activity could be enriched about 12-fold by fractional ammonium sulfate precipitation. Besides the enniatin synthetase system this represents another example of the cell-free synthesis of a depsipeptide from eucaryotic origin.
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D047630:Depsipeptides; D007700:Kinetics; D003904:Mitosporic Fungi / Q000378:metabolism*; D010455:Peptides; D010456:Peptides, Cyclic / Q000096:biosynthesis; D010649:Phenylalanine / Q000378:metabolism; D014633:Valine / Q000378:metabolism
D000900:Anti-Bacterial Agents; D002250:Carbon Radioisotopes; D047630:Depsipeptides; D010455:Peptides; D010456:Peptides, Cyclic; C004456:beauvericin; D010649:Phenylalanine; D014633:Valine