Acceleration of cytokinesis in PtK1 cells treated with microtubule inhibitors.
Mitotic PtK1 cells were treated at furrow initiation with microtubule poisons to determine the role of microtubules in the regulation of terminal cytokinetic events. The administration of anti-microtubule agents in late anaphase accelerated the rate of cytokinesis by approx. 60% as measured by changes in furrow diameter. Application of colcemid, nocodazole, or vinblastine sulfate to cells at furrow initiation all increased the rate of furrowing. Nocodazole, applied at various concentrations, demonstrated a dose-dependent relationship with furrowing rate. These results suggest a coupling between the disorganization and depolymerization of microtubules and the acceleration of furrowing. Electron microscopic analysis of cells treated with microtubule inhibitors show approx. 70% fewer microtubule profiles in the constricted region between the daughter cells. Microtubules may play a restraining role in the rate of furrowing.
D000705:Anaphase; D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003599:Cytoskeleton / Q000648:ultrastructure; D003703:Demecolcine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D007614:Macropodidae; D008870:Microtubules / Q000502:physiology* / Q000648:ultrastructure; D015739:Nocodazole; D014747:Vinblastine / Q000494:pharmacology*
D001562:Benzimidazoles; D014747:Vinblastine; D015739:Nocodazole; D003703:Demecolcine