A novel Japanese Burkitt's lymphoma cell line, P32/Ishida, with a new variant chromosomal translocation (2;14).
A novel cultured cell line, P32/Ishida, was established from a Japanese 8-year-old boy with abdominal Burkitt's lymphoma. The P32/Ishida cells were proved to have immature B-cell phenotypes on the basis of immunological surface marker analysis: surface immunoglobulins (gamma, mu, kappa), Ia-like antigen, B1 antigen, and common acute lymphoblastic leukemia antigen were positive. A small amount of IgM, but no IgG, could be detected in both cell extract and culture supernate of P32/Ishida cells. Cytogenetic studies revealed that the P32/Ishida cells consisted of cells having three derivative karyotypes characterized by common marker chromosomes, dup(1), and 14q + due to a new variant chromosomal translocation (2;14), which has not been identified in usual Burkitt's cell lines. Epstein-Barr virus nuclear antigen and terminal deoxynucleotidyl transferase activity were confirmed to be negative.
D000911:Antibodies, Monoclonal / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D002051:Burkitt Lymphoma / Q000235:genetics* / Q000276:immunology / Q000648:ultrastructure; D002460:Cell Line; D002478:Cells, Cultured; D002648:Child; D002900:Chromosomes, Human, 1-3; D002901:Chromosomes, Human, 13-15; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007564:Japan; D007621:Karyotyping; D008297:Male; D014178:Translocation, Genetic
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D007136:Immunoglobulins