title: Effect of colchicine on rat small intestinal absorptive cells. II. Distribution of label after incorporation of [3H]fucose into plasma membrane glycoproteins.
abstract: By means of radioautography the influence was tested of various periods (5, 15, 30, 40 min, 2 hr) of pretreatment with colchicine, administered intraperitoneally to rats at a dosage of 0.5 mg/100 g of body weight, on the intracellular pathway of [3H]fucose in absorptive cells of the small intestine. Administration of colchicine for 30 min and longer time intervals causes delay in the insertion of [3H]fucose into the oligosaccharide chains of glycoconjugates in the Golgi apparatus, and results in redistribution of the label apparent over the different portions of the plasma membrane. In controls, at 2 and 4 hr after administration of [3H]fucose the apical plasma membrane is strongly labeled; 53.7 +/- 3.2% of the silver grains are recorded over apical regions of the plasma membrane that contrast to basolateral portions comprising 25.4 +/- 3.2% of the label. Colchicine causes equalization of the reaction of apical and basolateral regions of the plasma membrane: the number of silver grains attributable to the apical plasma membrane is reduced; following treatment with colchicine, apical portions of the plasma membrane comprise 31.6 +/- 1.8% of the silver grains, 38.6 +/- 3.8% are attributable to basolateral membrane regions. The colchicine-induced equalization of the density of label of apical and basolateral regions of the plasma membrane, in addition to the occurrence of basolateral microvillus borders (demonstrated in the companion paper), suggests microtubules to be important in the maintenance of the polar organization of small intestinal absorptive cells.
mesh_terms: D000818:Animals; D001345:Autoradiography; D003078:Colchicine / Q000633:toxicity*; D005260:Female; D005643:Fucose / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis*; D007408:Intestinal Absorption / Q000187:drug effects*; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008565:Membrane Proteins / Q000096:biosynthesis*; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
chemical_list: D006023:Glycoproteins; D008565:Membrane Proteins; D014316:Tritium; D005643:Fucose; D003078:Colchicine