Hemin inhibits transfer of pre-delta-aminolevulinate synthase into chick embryo liver mitochondria.
Pulse labelling studies in chick embryo livers show that hemin prevents the transfer of drug induced pre-delta-aminolevulinate synthase from the cytosol into the mitochondria, leading to an accumulation of precursor in the cytosol. No effect of hemin was observed on the transfer of pre-pyruvate carboxylase into mitochondria. These results eliminated a general toxic effect of hemin on mitochondrial import of proteins and are consistent with the view that hemin specifically inhibits the transfer of ALA synthase.
D000624:5-Aminolevulinate Synthetase / Q000235:genetics*; D000818:Animals; D002642:Chick Embryo; D003600:Cytosol / Q000201:enzymology; D004792:Enzyme Precursors / Q000235:genetics*; D006418:Heme / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology*; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D011766:Pyruvate Carboxylase / Q000235:genetics
D004792:Enzyme Precursors; D006418:Heme; D000624:5-Aminolevulinate Synthetase; D011766:Pyruvate Carboxylase