Isolation and characterization of vitamin A-sensitive Chinese hamster lung cell lines.
Retinyl acetate (RA)-sensitive variants (RAs-2 and RAs-3) of V79 cell line were isolated after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. The variants were stable and showed a 3- to 4-fold increase in sensitivity to RA compared to parental V79 cells. The RAs-2 clone was also sensitive to retinol and retinol palmitate. The RA-sensitivity behaves as a recessive trait in all hybrids of RAs-2 and V79. A number of physiological parameters were indistinguishable in V79 and RAs-2 cells, including the extent of uptake of [3H]retinol, the release of K+ from the cells induced by RA, and the levels of retinol and retinoic acid binding proteins. However, one possible correlation with the RA-sensitive phenotype was observed: Gomori acid-phosphatase staining of RA-treated RAs-2 and V79 cells indicated that lysosomal membrane of RAs-2 cells was more labile than those of the parental V79 cells.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004224:Diterpenes; D005799:Genes, Dominant; D006822:Hybrid Cells / Q000502:physiology; D007425:Intracellular Membranes / Q000502:physiology; D008247:Lysosomes / Q000502:physiology; D012176:Retinoids / Q000494:pharmacology; D012177:Retinol-Binding Proteins / Q000502:physiology; D000084562:Retinyl Esters; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000494:pharmacology
D004224:Diterpenes; D012176:Retinoids; D012177:Retinol-Binding Proteins; D000084562:Retinyl Esters; D014801:Vitamin A; C009166:retinol acetate