Ultrastructural studies of the mouse aorta and its endothelial pinocytosis in diet-induced arteriosclerosis.
In mice fed on an atherogenic diet for 4 to 8 months, the aortas were examined by transmission and scanning electron microscopy. After 8 months of being fed a high cholesterol diet, the animals developed aortic intimal lesions composed mainly of proliferated modified smooth muscle cells with an increase of connective tissues. Scanning electron microscopy showed changes in aortic luminal surface consisting primarily of altered distribution of microvilli. Quantitative analysis of these changes showed a statistically significant (P less than 0.001) increase in cholesterol-fed animals compared with controls and a significant (P less than 0.001) difference between at 4 months and at 8 months in cholesterol-fed mice. Ultrastructural study on the uptake of protein tracer, horseradish peroxidase (HRP), by aortic endothelial cells in vitro was performed. The uptake of HRP was essentially the same for controls and cholesterol-fed mice at 4 months, but a statistically significant (P less than 0.005) increased uptake of HRP was observed at 8 months. Additional mice were subjected to nephrectomy with DOCA administration for 4 months along with a cholesterol-feeding. These animals showed a 2-fold increase in HRP-uptake compared with nephrectomized mice without a cholesterol-feeding. These results suggest that the enhanced pinocytotic activity of aortic endothelial cells in vitro, especially in hypertensive condition, and altered distribution of microvilli might be correlated to the arteriosclerotic process.
D000818:Animals; D001011:Aorta / Q000648:ultrastructure*; D001161:Arteriosclerosis / Q000473:pathology*; D002784:Cholesterol / Q000097:blood; D004036:Diet, Atherogenic; D004727:Endothelium / Q000648:ultrastructure; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009392:Nephrectomy; D010873:Pinocytosis
D002784:Cholesterol