Cellular factor stimulating DNA synthesis in nuclei isolated from sea urchin embryos.
DNA synthesis in isolated nuclei of morula-stage embryos of sea urchin was studied. Embryonic extracts of cleaving embryos (but not unfertilized eggs) stimulated DNA synthesis in the in vitro system. A stimulatory factor was identified which eluted at 0.52 M KCl during chromatography on DEAE-cellulose column. This factor was inactivated by heat treatment and trypsin digestion, and was resolved into three active peaks by gel filtration (Strokes radii of 6.3, 4.6, and 4.1 nm, respectively).
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis*; D002850:Chromatography, Gel; D004261:DNA Replication / Q000187:drug effects*; D005260:Female; D007700:Kinetics; D010063:Ovum / Q000032:analysis* / Q000166:cytology; D012617:Sea Urchins; D013942:Thymine Nucleotides / Q000378:metabolism; D014020:Tissue Extracts / Q000494:pharmacology*
D013942:Thymine Nucleotides; D014020:Tissue Extracts; C024157:thymidine 5'-triphosphate