A rapid and highly sensitive solid-phase radioassay for plasminogen activators.
A rapid and highly sensitive solid-phase radioassay for the measurement of plasminogen activators is presented. The method employs a convenient and stable 125I-fibrinogen-latex bead product and can reproducibly detect 0.25 milli Ploug units/ml of urokinase. This represents a 100-fold increase in sensitivity over previously published radioisotopic solid-phase technique and a 120-fold increase over the sensitivity of the fibrin plate method. Since the assay can readily detect plasminogen activator levels in euglobulin solutions prepared from pre- and post-venous occlusion plasma, it may be useful for rapidly detecting and monitoring the fibrinolytic potential of patients predisposed to thromboembolic disease.
D005340:Fibrinogen; D006801:Humans; D007457:Iodine Radioisotopes; D007840:Latex; D010960:Plasminogen Activators / Q000032:analysis*; D013923:Thromboembolism / Q000175:diagnosis
D007457:Iodine Radioisotopes; D007840:Latex; D005340:Fibrinogen; D010960:Plasminogen Activators