Hydroxylation of the C-8 position of deoxyguanosine by reducing agents in the presence of oxygen.
The C-8 position of deoxyguanosine was hydroxylated by various reducing agents in the presence of oxygen in 0.1 M phosphate buffer (pH 6.8) at 37 degrees C. As reducing agents, ascorbic acid, hydroxylamine, hydrazine, sodium bisulfite and dihydroxymaleic acid were used. Metal powder (Fe, Ni) or metalethylenediaminetetraacetic acid (EDTA) complex (FeII-EDTA, TiIII-EDTA) was also effective for the hydroxylation reaction in the presence of oxygen. Guanine residues in calf thymus DNA were also modified by these reagents. The possible biological significance of the hydroxylation of guanine residue in DNA in relation to mutagenesis and carcinogenesis is discussed.
D001205:Ascorbic Acid; D003849:Deoxyguanosine; D006900:Hydroxylation; D010084:Oxidation-Reduction; D010100:Oxygen
D003849:Deoxyguanosine; D001205:Ascorbic Acid; D010100:Oxygen