title: Alteration of the electrophoretic mobility of human peripheral blood mononuclear cells following treatment with dimethyl sulfoxide.
abstract: Studies have been conducted to determine the effects of DMSO and freezing on the electrophoretic distribution of peripheral blood mononuclear cells. Sodium [51Cr]chromate was used to label the cells, and the distributions of cell number and cell-associated radioactivity were determined. Cells treated with DMSO had a narrower distribution of electrophoretic mobilities when compared with those not treated. DMSO-treated cells also demonstrated a more homogeneous distribution of radioactivity relative to the cell distribution than did the nontreated cells. The freezing of DMSO-treated cells did not result in any additional alteration of electrophoretic pattern compared to DMSO treatment alone. Analysis by linear categorization techniques indicated that the DMSO-treated and nontreated cells were completely distinguished by their electrophoretic behavior.
mesh_terms: D001793:Blood Preservation; D002860:Chromium Radioisotopes; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004586:Electrophoresis; D005615:Freezing; D006801:Humans; D009000:Monocytes
chemical_list: D002860:Chromium Radioisotopes; D004121:Dimethyl Sulfoxide