Distribution of serum antibodies to wheat gliadin and bovine milk in atopic and non-atopic healthy adults.
Sera from 262 blood donors were tested against wheat gliadin and bovine milk antigens employing an ELISA system. 9.2% of the sera clearly contained antibodies, predominantly IgG but also including IgA and occasional IgM, reactive with either gliadin (11/262) or milk (13/262). There was no overlap between the gliadin and milk groups, and only one of these sera was also IgE-RAST-positive against wheat or milk. Five of 48 sera with raised IgE levels (greater than or equal to 100 iu/ml) were ELISA-positive against either gliadin or milk, a frequency comparable with that overall in this normal adult population. Six of these 48 sera with raised IgE levels were IgE-RAST-positive against wheat or milk, of which only one was ELISA-positive. These results have indicated that nearly 10% of normal adults have serum IgG/A/M antibodies to milk or gliadin, but that this population is separate from individuals (2.3%) who may be IgE-RAST-positive to the same dietary antigens.
D000293:Adolescent; D000328:Adult; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001666:Binding Sites, Antibody; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology*; D005903:Gliadin / Q000276:immunology*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008892:Milk / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology*; D011817:Rabbits; D011852:Radioallergosorbent Test
D000906:Antibodies; D010940:Plant Proteins; D007073:Immunoglobulin E; D005903:Gliadin