A new procedure for calculating the kinetic parameters of enzyme reactions.
The possibilities of computer and some mathematical and statistical considerations may renew the interest and reevaluate the advantages of the double reciprocal plot proposed by Lineweaver and Burk. From the experimental values of substrate concentration and reaction velocity, the best-fitting line can be statistically obtained using nonparametric criteria. Computer programs in FORTRAN and BASIC permit easy and elegant resolutions.
D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D008954:Models, Biological; D012984:Software
D004798:Enzymes