Immobilization of xanthine oxidase to controlled pore-glass. Application to high-performance liquid chromatography.
Immobilized xanthine oxidase (XO) was prepared by intermolecular cross-linking to controlled pore-glass using glutaraldehyde. The performance of enzyme reactors packed with the immobilized XO were considered theoretically and investigated experimentally. Using a post-column reactor packed with the immobilized XO, simultaneous determinations of hypoxanthine and xanthine by reversed-phase high-performance liquid chromatography were achieved.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004800:Enzymes, Immobilized / Q000378:metabolism; D005898:Glass; D007042:Hypoxanthines; D007700:Kinetics; D011685:Purine Nucleotides; D014969:Xanthine Oxidase / Q000378:metabolism; D014970:Xanthines
D004800:Enzymes, Immobilized; D007042:Hypoxanthines; D011685:Purine Nucleotides; D014970:Xanthines; D014969:Xanthine Oxidase