[A study of temperature sensitivity of choriocarcinoma cells in vitro--their cell proliferation and morphology in hyperthermic environment].
The possibility of hyperthermic therapy for choriocarcinoma was fundamentally analyzed in an in vitro study. The temperature sensitivity of choriocarcinoma cells designated GCH-nu-YS clone was examined using a severely controlled temperature gradient incubator (T.G.I.). The cells were placed into an atmosphere at various temperatures (37.0-41.2 degrees C) for about 2 weeks and the inhibitory effect on cell proliferation was chronologically investigated in addition to degenerative changes in cellular morphology. When the temperature was not higher than 39.2 degrees C the proliferation of YS clone was nearly the same as that of the cells cultured at 37.0 degrees C. Proliferation at 39.5-39.6 degrees C, however, was slightly inhibited. And when cultured at over 39.9 degrees C, it was markedly inhibited. Above 40.4 degrees C, cell proliferation was impossible, thus resulting in destruction of the cells. These data suggest that the temperature upper limit for viability (TLV) is approximately 40.4-40.6 degrees C and the temperature upper limit for proliferation (TLP) is approximately 40.1-40.2 degrees C. When Okumura's method of classification according to temperature sensitivity is applied, the choriocarcinoma cells may be regarded as having the characteristics of adenocarcinoma. Morphological findings for cells treated at TLV revealed enlargement of the cytoplasm, an increase in the number of perinuclear granules and the appearance of deformed cells such as bizarre cells.
D002470:Cell Survival; D002478:Cells, Cultured; D002822:Choriocarcinoma / Q000473:pathology*; D005260:Female; D006358:Hot Temperature; D006801:Humans; D008938:Mitosis; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000473:pathology*