title: Simulating certain aspects of hypogravity: effects on bone maturation in the non-weight bearing skeleton.
abstract: This study reports the effects of simulation of certain aspects of hypogravity (via partial skeletal unloading) on the growth and maturation of the non-weight bearing mandibles of 41-d and 1-yr-old rats. Partial skeletal unloading was effected by elevating the hindquarters (PULEH), and this simulation was controlled with normally loaded animals fed either ad libitum or the average amount of food consumed by the the experimental group (group-mean fed). The chemical status of the mandibles after 10 d or 14 d PULEH closely resembled that of control rats. The younger PULEH rats and their group-mean fed controls demonstrated a trend toward impaired maturation of mineral and matrix moieties; yet the concentrations of calcium (Ca) and phosphorus (P) expressed as a ratio to collagen hydroxyproline content were normally distributed within a density gradient profile which separates the mineral and matrix moieties into various age-dependent fractions. These data demonstrate that 10 d or 14 d PULEH in young or old rats, respectively, is not sufficient to elicit the maturation deficit observed in the mandibles of rats flown for 18.5 d in the Soviet Biosatellite Cosmos-1129. Unless the duration of PULEH is critical, the cephalad fluid shift which is common to PULEH and spaceflight animals cannot be solely responsible for the flight-induced maturation deficit. Because the mandibles of the PULEH rats remain antigravity-postured, the results emphasize the importance of gravity unloading to the impairment of mandibular bone matrix/mineral maturation during spaceflight. Decreased gravity and, hence, gravity unloading cannot be mimicked in ground-based models of hypokinesia.
mesh_terms: D000367:Age Factors; D000818:Animals; D002118:Calcium / Q000032:analysis*; D006112:Gravitation; D008297:Male; D008334:Mandible / Q000032:analysis / Q000254:growth & development*; D010758:Phosphorus / Q000032:analysis*; D011187:Posture; D051381:Rats; D011919:Rats, Inbred Strains; D012149:Restraint, Physical; D013026:Space Flight; D013046:Specific Gravity; D013997:Time Factors; D014893:Weightlessness / Q000009:adverse effects*
chemical_list: D010758:Phosphorus; D002118:Calcium