title: Rat ligandin mRNA molecular cloning and sequencing.
abstract: Recombinant plasmids containing the double-stranded cDNA sequences of mRNA for the Mr 22,000 ligandin (glutathione S-transferase B) subunit (Ya) have been constructed. The DNA sequence of an insert corresponding to the middle and 3' regions of the mRNA was determined and an amino acid sequence was proposed for the ligandin Ya subunit. The proposed sequence reveals a high content of basic amino acids (Arg and Lys) and Leu, is consistent with the amino acid composition, and predicts the correct number of peptides derived from tryptic digests reported for ligandin.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D005982:Glutathione Transferase / Q000235:genetics* / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D012333:RNA, Messenger; D004247:DNA; D005982:Glutathione Transferase