title: Time-lapse cinemicrography and scanning electron microscopy of platelet formation by megakaryocytes.
abstract: The surface architecture of megakaryocytes undergoing platelet formation in vitro has been examined by time-lapse cinemicrography and scanning electron microscopy. Fragments of mouse bone marrow were placed in culture medium and incubated at 37 degrees C. After several hours mature megakaryocytes migrated out of the marrow and some underwent shape changes so that they eventually appeared as a relatively small central body, housing the nucleus, from which emerged a number of thin processes which resembled platelet chains. Scanning electron microscopy showed that initially the megakaryocyte surface was ruffled but with development of processes it became smoother. Circumferential folds of small amplitude were found on the surface of developing constrictions which separated putative platelets. It is thought they may be associated with the mechanism of extension, but could have a role in establishing the topography of membrane components. Rupture of the chains and release of platelets was not observed; this permits the number of putative platelets formed by individual megakaryocytes to be determined. The putative platelets exhibited features common to circulating platelets when exposed to a glass surface including the development of pseudopodia and, eventually, flattening on to the surface.
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000648:ultrastructure*; D001853:Bone Marrow / Q000648:ultrastructure; D002462:Cell Membrane / Q000648:ultrastructure; D005614:Freeze Fracturing; D008533:Megakaryocytes / Q000648:ultrastructure*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009040:Motion Pictures