The use of a zwitterionic detergent in two-dimensional gel electrophoresis of trout liver microsomes.
CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-Propane sulfonate, a zwitterionic detergent, has been shown to exhibit superior membrane protein solubilizing characteristics as compared to nonionic detergents. Replacement of NP-40 with CHAPS in isoelectric focusing of rainbow trout liver microsomes has increased resolution markedly. The two-dimensional electrophoretic system described will allow effective resolution of up to 300 micrograms of crude microsomal protein. CHAPS exhibits no effect on the stability or type of pH gradient when compared to NP-40 during isoelectric focusing in the presence of urea.
D000818:Animals; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005399:Fishes; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008862:Microsomes, Liver / Q000032:analysis*; D011506:Proteins / Q000302:isolation & purification*; D013501:Surface-Active Agents
D003902:Detergents; D011506:Proteins; D013501:Surface-Active Agents