Purified DNA polymerase gamma replicates bovine parvovirus DNA to a unit-length product.
DNA polymerase gamma, purified from fetal bovine liver, replicated virion single-stranded DNA from bovine parvovirus to a unit-length double-stranded DNA molecule. This product was not nicked and was covalently linked to the 3' hairpin primer. The reaction was inhibited by dideoxythymidine 5'-triphosphate, but was unaffected by ATP or aphidicolin. Double-stranded viral DNA was not a functional template for purified DNA polymerase gamma.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D004258:DNA Polymerase III / Q000302:isolation & purification / Q000378:metabolism*; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D005260:Female; D008099:Liver / Q000201:enzymology; D010321:Parvoviridae / Q000235:genetics*; D011247:Pregnancy
D004279:DNA, Viral; D000255:Adenosine Triphosphate; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase