Interactions between tumor cells and vascular endothelium.-II) Light and electron microscopic studies.
Electron microscope technique was used to investigate the passage across the endothelial monolayer by murine tumor lines. After the initial adhesion, cancer cells induce a retraction of endothelium and migrate under vascular intima. Subsequently they spread on basement membrane showing a flattened shape, meanwhile endothelial cells reconstitute the monolayer. The four tumor lines show a similar behaviour being able to induce endothelial retraction and exposure of extracellular matrix and cross through the monolayer. The technique appears useful to study in details this multi-step process.
D000818:Animals; D002460:Cell Line; D004727:Endothelium / Q000648:ultrastructure*; D006801:Humans; D007231:Infant, Newborn; D051379:Mice; D008854:Microscopy, Electron; D009369:Neoplasms / Q000648:ultrastructure*; D014471:Umbilical Veins / Q000648:ultrastructure*