A method for the ligation of DNA following isolation from low melting temperature agarose.
A rapid, simple, and reliable method is presented for the isolation and subsequent ligation of DNA from agarose gels. The technique involves the use of low melting temperature agarose, but with the inclusion of bovine serum albumin or gelatin to the ligation reaction.
D004247:DNA / Q000302:isolation & purification*; D011088:DNA Ligases; D004587:Electrophoresis, Agar Gel; D005780:Gelatin; D012710:Serum Albumin, Bovine; D013696:Temperature
D012710:Serum Albumin, Bovine; D005780:Gelatin; D004247:DNA; D011088:DNA Ligases