A selective liquid medium for primary isolation of South American Leishmanias.
A selective liquid medium for direct isolation of Leishmania spp. from mucocutaneous lesions is described. Minimal inhibitory concentrations and synergy studies were performed and used to select 5-fluorocytosine as an antimycotic agent which inhibits contaminating fungi without an adverse effect on the parasite thus permitting its isolation from contaminated lesions. Penicillin-Streptomycin were included as antibacterial agents. Isolations have been obtained from biopsy material in an average of 5.6 days from 11 of 15 patients with mucous lesions.
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D003470:Culture Media; D006801:Humans; D007891:Leishmania / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000469:parasitology*; D007897:Leishmaniasis, Mucocutaneous / Q000469:parasitology*; D009092:Mucous Membrane / Q000469:parasitology; D010274:Parasitology / Q000379:methods; D012867:Skin / Q000469:parasitology
D000890:Anti-Infective Agents; D003470:Culture Media