(23S)-1,23,25-Trihydroxycholecalciferol, an intestinal metabolite of 1,25-dihydroxycholecalciferol.
(23S)-23,25-Dihydroxycholecalciferol was converted into a polar metabolite in a calciferol-deficient chick kidney homogenate. The metabolite was identified as (23S)-1,23,25-trihydroxycholecalciferol by absorbance spectroscopy and mass spectrometry, and by formation of derivatives. (23S)-1,23,25-Trihydroxycholecalciferol was also observed as a 1,25-dihydroxycholecalciferol metabolite in intestinal cells isolated from 1,25-dihydroxycholecalciferol-treated rat. The trihydroxy metabolite was 50-fold less potent than 1,25-dihydroxycholecalciferol in the chick intestinal 1,25-dihydroxycholecalciferol receptor assay.
D000818:Animals; D002117:Calcitriol / Q000031:analogs & derivatives* / Q000378:metabolism*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry
C038789:1,23,25-trihydroxyvitamin D3; D002117:Calcitriol