[Interaction of DNA and hydrogen peroxide. A method of localizing pyrimidine bases in DNA].
Incomplete modification with hydrogen peroxide of the DNA fragments labeled at one terminus is shown to be useful for localisation of C and T residues along the polynucleotide chain. The fragments can be split at modified residues with piperidine after hydrogen peroxide treatment. The rate of the reaction of the single-stranded fragments is extremely fast, much higher than that of the double-stranded DNA. Splitting of the DNA takes place at C if pH 7,4, and at T at pH 9,6. The modification can be used to investigate the DNA structure and function.
D001483:Base Sequence; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D006861:Hydrogen Peroxide; D011742:Pyrimidine Nucleotides
D011742:Pyrimidine Nucleotides; D004247:DNA; D006861:Hydrogen Peroxide
Vzaimodeĭstvie DNK s perekis'iu vodoroda. Metod lokalizatsii pirimidinovykh osnovaniĭ v DNK.