Age-synchronous culture of Caenorhabditis elegans: technique and applications.
The inclusion of mobility to an age synchrony method and the development of an inexpensive filter device resulted in a natural model aging system without resorting to invasive chemical techniques. Large or small cohorts of nematodes with less than 1% offspring contamination are possible. The filter/mobility method is compared to other methods using the same strain and culture temperature. The applicability of the method is shown with a variety of parameters, and a previously reported parameter of aging in nematodes, i.e., specific gravity, is shown not to be an aging parameter of Caenorhabditis elegans.
D000375:Aging; D000818:Animals; D001699:Biometry; D002107:Caenorhabditis / Q000033:anatomy & histology / Q000254:growth & development* / Q000502:physiology; D046508:Culture Techniques / Q000379:methods; D005260:Female; D005298:Fertility; D010058:Oviposition; D011156:Population Density; D013997:Time Factors