Direct determination of sodium and potassium in blood serum by flow injection and atomic absorption spectrophotometry.
A simple and reliable method for the measurement of sodium and potassium in blood serum without any sample dilution by using flow injection and atomic absorption spectrophotometry is described. A sample throughout of 100 measurements per hour is possible. Matrix problems have not been encountered. The coefficient of variation for within-run determinations is about 1,14 and 2,36% for sodium and potassium, respectively, in serum samples (n = 10). The method is easily adaptable to pediatric research, because of the low required sample volume of 5 microliter.
D001800:Blood Specimen Collection; D006801:Humans; D011188:Potassium / Q000097:blood*; D012964:Sodium / Q000097:blood*; D013054:Spectrophotometry, Atomic / Q000379:methods*
D012964:Sodium; D011188:Potassium