[Transmission of amber mutants in bacteriophage T4. II. Thermal sensitivity of the multiplication of gene 26 amber mutants in Escherichia coli B cells and the absence of such sensitivity in the case of gene 33].
The dependence of multiplication of genes 26 and 33 amber mutants of T4D phage in a non-permissive host on the temperature in the range of 25-41 degrees C has been studied. The secondary mutations observed in the genome of gene 26 mutants amS105 and amNG114 were excluded by crossing these mutants with the wild type phage. It was established that multiplication of gene 26 amber mutants amS105x5, amN131 and amNG114x1 depends, essentially, on the temperature: the burst size of phage gradually decreased by about three orders, when the incubation temperature was changed from 25 degrees C to 41 degrees C. In the case of gene 33 amber mutants, their multiplication was only slightly dependent on the temperature, maximum burst size having been observed at 37 degrees C. The decrease in the burst size at 41 degrees C of gene 33 mutant amc18 was shown to be caused by secondary ts mutation in the phage genome. It is concluded that the phenomenon of the temperature sensitivity of multiplication of T4D phage amber mutants is gene specific.
D003433:Crosses, Genetic; D005814:Genes, Viral; D009154:Mutation; D013604:T-Phages / Q000235:genetics*; D013696:Temperature; D014779:Virus Replication
Izuchenie transmissii amber-mutantov bakteriofaga T4. Soobshchenie II. Termochuvstvitel'nost' razmnozheniia amber-mutantov po genu 26 v kletkakh Escherichia coli B i otsutstvie takovoĭ v sluchae gena 33.