title: Presence of three B-type cytochromes in swine cerebral microsomes.
abstract: In swine cerebral microsomes purified with sucrose density gradient and glycerol-cholate gradient centrifugations, it was observed that a new b-type cytochrome which had alpha-peak at 560 nm and Soret peak at 428 nm at 23 degrees C was reduced preferentially by anaerobic NADPH in the presence of cyanide. The b5-type cytochromes were reduced completely by both NADH and NADPH anaerobically. Three b-type cytochromes were partially purified into two b-type, spectroscopically distinct from each other, and the new b-type (b560-5) cytochromes.
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D003573:Cytochrome b Group / Q000302:isolation & purification* / Q000378:metabolism; D015786:Cytochromes b5; D042963:Electron Transport Complex II; D007700:Kinetics; D008861:Microsomes / Q000378:metabolism*; D010084:Oxidation-Reduction; D013053:Spectrophotometry; D013552:Swine
chemical_list: D003573:Cytochrome b Group; C025355:cytochrome b560; D015786:Cytochromes b5; D042963:Electron Transport Complex II