[Immunotherapy of postoperative cancer patients with immune RNA-sensitized lymphocytes and tumor antigen].
Tumor cell suspension was prepared from resected tumor tissue of various cancer patients. 1-RNA was extracted from lymphoid tissues of rabbits immunized with each tumor cells and CFA. When the postoperative condition of patient recovered stable, autologous lymphocytes were prepared with blood cell separator (IBM 2997 type) and incubated with I-RNA, then, return to himself. Total number of sensitized lymphocytes was 4 X 10(9) - 1.2 X 10(10). After this passive immunization was finished, active immunization with autologous 3M KCl tumor extract was added. Three of gastrointestinal cancer, 3 of lung cancer, 2 of miscellaneous malignant tumor were treated with this schedule. T-cell ratio, blastogenesis of T-cell with PHA, skin reaction with PPD and PHA, CEA, IAP and Ig in serum was not definitely changed before and after the immunotherapy. But 3 of them, LAI AND LMI became to positive after the treatment and NK cell activity also increased in 3 cases. In these 3 cases, tumor regression was recognized after the treatment. In a case of synovial sarcoma, skin reaction for autologous tumor antigen became to positive and the metastatic lesion in the lung was gradually regressed and regression is still continuing 6 months after the treatment (reduction ratio 90%).
D000368:Aged; D000951:Antigens, Neoplasm / Q000008:administration & dosage*; D003110:Colonic Neoplasms / Q000276:immunology / Q000601:surgery / Q000628:therapy; D005260:Female; D006801:Humans; D007116:Immunization, Passive; D008175:Lung Neoplasms / Q000276:immunology / Q000601:surgery / Q000628:therapy*; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011184:Postoperative Period; D012334:RNA, Neoplasm / Q000276:immunology*
D000951:Antigens, Neoplasm; D012334:RNA, Neoplasm