title: Stimulation of sugar transport in cultured heart cells by triiodothyronine (T3) covalently bound to red blood cells and by T3 in the presence of serum.
abstract: T3, covalently bound to red blood cells (RBCs), stimulated the uptake rate of 2-deoxy-D-glucose (2-DOG) in cultured chick embryo heart cells. The response, measured 6 h after exposure, was at least the same than that to free T3. An inhibitor of rhodamine-T3 internalization, bacitracin, did not affect the stimulation of sugar uptake by T3 regardless of whether T3 was covalently bound or free. Pretreatment of RBC-T3 with anti-T3 immunoglobulin G completely blocked the effect of T3, whereas normal rabbit immunoglobulin G failed to do so. Addition of 5% chick serum to the medium stimulated 2-DOG uptake to 144% of the control at 6 h. Adding T3 (10 nM) to the serum-containing medium increased 2-DOG uptake to 171% of the control. The effect of T3 alone or in the presence of serum was not inhibited by cycloheximide, puromycin, or actinomycin D. A T3 dose response curve, in medium containing 10% dehormonized serum, showed enhancement of the T3 effect when compared with the curve obtained in the serum-free medium. The minimal effective concentration of T3 was 10 pM in the presence of serum and 100 pM in its absence. The slope of the linear portion of the dose response curve was greatly increased and the maximal response markedly enhanced by serum. The ED50 was 0.33 nM vs. 0.43 nM in terms of total T3 concentration and 0.16 nM vs. 0.43 nM in terms of free T3 in the presence or absence of serum, respectively. These data suggest that T3, in physiological concentrations, activates sugar transport through an external contact with the cell surface.
mesh_terms: D000818:Animals; D001414:Bacitracin / Q000494:pharmacology; D002352:Carrier Proteins / Q000378:metabolism*; D002642:Chick Embryo; D003847:Deoxyglucose / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D006321:Heart / Q000187:drug effects; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009004:Monosaccharide Transport Proteins; D009206:Myocardium / Q000378:metabolism* / Q000648:ultrastructure; D014284:Triiodothyronine / Q000097:blood / Q000494:pharmacology*
chemical_list: D002352:Carrier Proteins; D009004:Monosaccharide Transport Proteins; D014284:Triiodothyronine; D001414:Bacitracin; D003847:Deoxyglucose