[Alkaline phosphatase activity and isoenzymes in the blood serum of cattle, sheep and swine].
Studied were the total activity and the type of isoenzymes of the alkaline phosphatase in blood serum of cattle, sheep, and swine at various ages and different physiologic status. With all three species the enzyme activity showed explicit age dynamic. In month-old calves the activity of the serum phosphatase was 82.8 IU, on an average, and in adult cows--32.2 IU. In month-old lambs the enzyme activity was 310.6 IU, on an average, while in adult sheep it was 62.0 IU. In young pigs it ranged up to 105.0 IU, and in adult swine--up to 32.4 IU. No statistically significant differences in the enzyme activity was established between dry and lactating cows and ewes as well as between rams, boars, and sows. The study on the isoenzymes of the alkaline phosphatase (thermostability, specific chemoinhibitors, and electrophoresis in agar gel) revealed that in month-old calves there was only a bone isoenzyme in the blood serum; in calves that were more advanced in age there appeared also the liver and the intestinal isoenzymes, while in adult cows the intestinal or the liver enzyme of alkaline phosphatase was present only. The blood serum of lambs up to the sixth month of age contained the bone and the intestinal isoenzymes, and that of adult sheep--the liver and the intestinal ones. In pregnant sheep there were even low amounts of the placental isoenzyme of alkaline phosphatase. In pigs up to the sixth month of age the serum phosphatase was shown to be of bone origin, while in the adult swine--chiefly of liver origin.
D000375:Aging; D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D002417:Cattle / Q000097:blood*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004587:Electrophoresis, Agar Gel; D005260:Female; D007527:Isoenzymes / Q000097:blood*; D008297:Male; D011247:Pregnancy; D012756:Sheep / Q000097:blood*; D013552:Swine / Q000097:blood*
D007527:Isoenzymes; D000469:Alkaline Phosphatase
Aktivnost i izoenzimi na alkalnata fosfataza v krŭven serum ot goveda, ovtse i svine.