A simplified technique for the isolation of the fourth complement component.
Prothrombin complex (P.C.) preparations obtained by batch adsorption onto DEAE-Sephadex are highly enriched in C4. Based on this observation a technique has been elaborated where the P.C. analogue is further submitted to precipitation by ethanol and batch adsorption of impurities on DEAE-Cellulose. Unaltered C4 is obtained in a 2 days process with a yield corresponding to 40% of the starting material (cryoconcentrate supernatant).
D001779:Blood Coagulation Factors / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose; D003181:Complement C4 / Q000302:isolation & purification*; D004586:Electrophoresis; D006461:Hemolysis; D006801:Humans; D007122:Immunoelectrophoresis; D008970:Molecular Weight
D001779:Blood Coagulation Factors; D003181:Complement C4; C025667:prothrombin complex concentrates