Freedom of coccidial oocysts from Newcastle disease virus.
Oocysts were recovered from chickens experimentally infected with the Mukteswar strain of Newcastle disease virus and Eimeria acervulina or E tenella. Oocysts sterilised by sodium hypochlorite solution were washed, ruptured and inoculated into embryonating eggs which were examined after five days by the haemagglutination test for virus. All these tests were negative. Virus, initially detected with oocysts separated from faeces, was no longer present after sporulation in 2 per cent potassium bichromate. The B1 strain of virus survived for up to 30 hours in 2 per cent sodium hydroxide and 2 per cent potassium bichromate but was quickly destroyed by sodium hypochlorite (specific gravity 1.075).
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D003044:Coccidia / Q000382:microbiology*; D003048:Coccidiosis / Q000150:complications / Q000662:veterinary*; D009521:Newcastle Disease / Q000150:complications* / Q000635:transmission; D009522:Newcastle disease virus / Q000187:drug effects; D011201:Poultry Diseases / Q000382:microbiology*; D012973:Sodium Hypochlorite / Q000494:pharmacology
D012973:Sodium Hypochlorite