Changes of ATP, polyphosphate and K+ contents in Saccharomyces carlsbergensis during uptake of Mn2+ and glucose.
The process of prolonged Mn2+ uptake by the yeast Saccharomyces carlsbergensis in the presence of 100 mM glucose and in the absence of phosphate can be divided into two steps. The first step (0-20 min) of Mn2+ uptake (4.3 mumol/g of wet cells) is characterized by an intense K+ efflux (23.8 mumol/g), synthesis of high molecular weight polyphosphate (HPP) (8.1 mumol/g) and decrease of ATP content (0.06 mumol/g). Simultaneously about 0.6 mumol of glucose is taken up and the level of low molecular weight polyphosphate (LPP) remains practically unchanged. The second step (20-120 min) of Mn2+ uptake (15.6 mumol/g) is characterized by a drop in HPP (16.6 mumol/g) and the synthesis of LPP (19.0 mumol/g). The ATP content decreases by 0.87 mumol/g as compared to the control, while that of K+ increases (5.7 mumol/g). During the first step of Mn2+ uptake the energy of the K+ concentration gradient may be used both for Mn2+ influx (2K+: 1Mn2+) and synthesis of HPP (1P:1.9K+). During the second step the Mn2+ accumulation is apparently driven by HPP conversion into LPP (1:1) and by ATPases serving the Mn2+/H+ exchange.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D001693:Biological Transport, Active; D002412:Cations; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D008345:Manganese / Q000378:metabolism*; D008970:Molecular Weight; D011122:Polyphosphates / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D012440:Saccharomyces / Q000378:metabolism*
D002412:Cations; D011122:Polyphosphates; D008345:Manganese; D000255:Adenosine Triphosphate; D005947:Glucose; D011188:Potassium