Determination of the underivatized antineoplastic drugs cyclophosphamide and 5-fluorouracil and some of their metabolites by capillary gas chromatography combined with electron-capture and nitrogen-phosphorus selective detection.
A rapid and sensitive method for the determination of cyclophosphamide (CP) and 5-fluorouracil (5-FU) and some of their metabolites in one analysis has been developed. Surface-coated open-tubular OV-275 columns were combined with electron-capture detection and nitrogen-phosphorus selective detection. The influence of the column diameter on the separation is shown. Extraction with 2-propanol-diethyl ether (22:77) allows the isolation of CP, 5-FU and their analogues in one extraction step. The assay was applied to some pharmacokinetic experiments with chemotherapeutically treated patients and with a WAG/Rij rat.
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D003520:Cyclophosphamide / Q000097:blood*; D004583:Electrons; D005472:Fluorouracil / Q000097:blood*; D006801:Humans; D009584:Nitrogen / Q000032:analysis; D010758:Phosphorus / Q000032:analysis; D051381:Rats; D013696:Temperature
D010758:Phosphorus; D003520:Cyclophosphamide; D009584:Nitrogen; D005472:Fluorouracil