Comparison of Bolton-Hunter and chloramine-T techniques for the radioiodination of prostatic acid phosphatase.
A double antibody, semi-automated radioimmunoassay for serum prostatic acid phosphatase (PAP) is described, which uses the 125I-labelled N-succinimidyl-3-(4-hydroxyphenyl) propionate ester of PAP. This type of label has substantially higher immunoreactivity than that prepared using the chloramine-T method of radioiodination. Chromatographic purification of either label on Ultrogel ACA44 further improved immunoreactivity. The lowest detection limit (0 . 35 micrograms/l) was achieved with the chromatographically purified ester label.
D000135:Acid Phosphatase / Q000097:blood*; D000818:Animals; D002700:Chloramines; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D008297:Male; D011467:Prostate / Q000201:enzymology*; D011817:Rabbits; D011863:Radioimmunoassay; D013388:Succinimides; D014105:Tosyl Compounds
D002700:Chloramines; D007457:Iodine Radioisotopes; D013388:Succinimides; D014105:Tosyl Compounds; C016300:chloramine-T; C022581:Bolton-Hunter reagent; D000135:Acid Phosphatase