Inhibition of the calcium- and phospholipid-dependent protein kinase activity from mouse brain cytosol by quercetin.
The flavonoid quercetin is a potent inhibitor of calcium- and phospholipid-dependent protein kinase (Ca, PL-PK) activity from mouse brain. Half-maximal inhibition of the kinase occurs at about 10 microM. If the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) is used instead of calcium as a stimulating factor of the kinase enzyme activity is still inhibited by quercetin. The kinase inhibitor, however, does not interfere with the binding of TPA to its receptor.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D003600:Cytosol / Q000201:enzymology; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D006657:Histones / Q000378:metabolism; D051379:Mice; D010718:Phosphatidylserines / Q000494:pharmacology; D011494:Protein Kinases / Q000378:metabolism*; D011794:Quercetin / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D005419:Flavonoids; D006657:Histones; D010718:Phosphatidylserines; D000255:Adenosine Triphosphate; D011794:Quercetin; D011494:Protein Kinases; D013755:Tetradecanoylphorbol Acetate