Transforming activity of human melanoma DNA.
Cellular DNA transfection to mouse NIH3T3 cells revealed transforming activity of human malignant melanoma DNA. The corresponding human sequence was cloned from the transformed mouse cells. By hybridization to viral oncogenes, the transforming gene in human melanoma was identified as a homologue of viral Ha-ras gene.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000032:analysis*; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008545:Melanoma / Q000235:genetics*; D051379:Mice; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D010957:Plasmids; D014162:Transfection
D004273:DNA, Neoplasm