Reduced levels of peroxisomal enzymes in the kidney of the genetically obese (ob/ob) mouse. Contrast with liver.
Kidney post-nuclear supernatants from genetically lean and obese mice were subjected to subcellular fractionation by dual centrifugation through sucrose gradients in B XIV zonal rotors. Considerable purification of peroxisomes was achieved which allowed the demonstration of acyl-CoA beta-oxidation enzymes and carnitine acyltransferases in these organelles. Comparison of kidney peroxisome-enriched fractions from obese and lean mice indicated a likely relative depression in beta-oxidation enzymes in the obese animal. Measurement of catalase, acyl-CoA oxidase and carnitine octanoyltransferase in whole homogenate of liver and kidney of obese and lean mice revealed significantly reduced levels (to approximately 2/3) of these peroxisomal enzymes in the kidney of ob/ob mice. In contrast the specific activity of catalase and acyl-CoA oxidase was significantly raised in the liver of obese mice.
D042962:Acyl-CoA Oxidase; D000818:Animals; D002333:Carnitine Acyltransferases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002458:Cell Fractionation; D002501:Centrifugation, Zonal; D006867:Hydrolases / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D008820:Mice, Obese / Q000378:metabolism*; D008830:Microbodies / Q000201:enzymology* / Q000648:ultrastructure; D009928:Organ Specificity; D010088:Oxidoreductases / Q000378:metabolism*
D010088:Oxidoreductases; D002374:Catalase; D042962:Acyl-CoA Oxidase; D002333:Carnitine Acyltransferases; C030162:carnitine octanoyltransferase; D006867:Hydrolases