title: Quantitation of digitoxin and the bis- and monodigitoxosides of digitoxigenin in serum.
abstract: A specific assay is described for measuring the concentration of digitoxin and the bis- and monoglycosides of digitoxigenin in serum. The procedure includes: (1) addition of a tracer amount of tritium labeled parent compound to the serum in order to measure percentage recovery; (2) solvent extraction to separate polar and non-polar metabolites; (3) reversed-phase thin-layer chromatography of the non-polar fraction to separate digoxigenins from digitoxigenins; (4) thin-layer chromatography to isolate digitoxin, and the bis- and monoglycosides of digitoxigenin; and (5) use of an 125I-radioimmunoassay to determine the concentration of the glycosides. Each of these three glycosides was administered intravenously to a normal subject, and the concentration of parent compound was measured in the serum at various times.
mesh_terms: D000328:Adult; D004073:Digitoxigenin / Q000031:analogs & derivatives* / Q000097:blood; D004074:Digitoxin / Q000097:blood* / Q000378:metabolism; D006801:Humans; D008297:Male; D011863:Radioimmunoassay / Q000379:methods; D013237:Stereoisomerism; D013997:Time Factors
chemical_list: D004073:Digitoxigenin; C038148:digitoxigenin-bis(digitoxoside); C035486:digitoxigenin monodigitoxoside; D004074:Digitoxin