title: Sequence of the hemagglutinin gene from influenza virus A/Seal/Mass/1/80.
abstract: A double-strand DNA copy of the influenza virus A/Seal/Mass/1/80 (H7N7) [seal] hemagglutinin (HA) gene was cloned into the plasmid pAT153/PvuII/8 and sequenced to deduce the primary amino acid sequence. The gene is 1731 nucleotides long and codes for a protein of 560 amino acids with a nonglycosylated molecular weight of 62098 Da. The deduced amino acid sequence displays similarities to all other sequenced hemagglutinins by retaining six of seven potential glycosylation sites, showing conversation in the number and position of cysteine residues, conservation in the fusion and anchor peptides, and conservation in the putative receptor site of the molecule. However, three features of the primary amino acid sequence could be distinguished from the H7 amino acid sequence of A/fowl plague/Rostock/34 (FPV), another avian H7 influenza virus which does not produce disease in mammals. First, the seal HA sequence has three fewer amino acids in the connecting peptide region of the HA than FPV. This lack of multiple basic amino acids in the connecting peptide is similar to that found in avirulent H7 avian strains and to mammalian serotypes H1, H2, and H3. Second, the seal HA has gained four additional proline residues, all in HA1, as compared to FPV. These residues may alter the tertiary structure of the HA and ultimately contribute to the biological features of this virus. Third, the seal HA has lost a potential carbohydrate attachment site at residue 149 which lies at the tip of the HA structure. The loss of this carbohydrate could alter the seal HAs interaction with host cell receptors.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA; D005814:Genes, Viral; D006389:Hemagglutinins, Viral / Q000032:analysis / Q000235:genetics*; D009980:Influenza A virus / Q000235:genetics* / Q000276:immunology; D011392:Proline / Q000032:analysis; D011487:Protein Conformation; D013329:Structure-Activity Relationship
chemical_list: D006389:Hemagglutinins, Viral; D004247:DNA; D011392:Proline