title: Glycoprotein properties of the solubilized atrial muscarinic acetylcholine receptor.
abstract: The muscarinic acetylcholine receptor from porcine atria exhibits sialoglycoprotein characteristics based on its sensitivity to neuraminidase digestion and its ability to interact specifically with lectin affinity resins when solubilized with a digitonin/cholate mixed detergent system. Differential lectin binding properties of the neuraminidase-treated and untreated receptor suggest that high-affinity binding to immobilized wheat germ agglutinin is accomplished through the presence of both terminal sialic acid and internal N-acetylglucosamine or its beta(1 leads to 4)-linked oligomers.
mesh_terms: D000818:Animals; D006023:Glycoproteins / Q000032:analysis*; D006325:Heart Atria / Q000032:analysis; D037102:Lectins / Q000378:metabolism; D009206:Myocardium / Q000032:analysis*; D009439:Neuraminidase / Q000378:metabolism; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000032:analysis*; D012995:Solubility; D013552:Swine; D014909:Wheat Germ Agglutinins
chemical_list: D006023:Glycoproteins; D037102:Lectins; D011950:Receptors, Cholinergic; D014909:Wheat Germ Agglutinins; D011813:Quinuclidinyl Benzilate; D009439:Neuraminidase