[Excision of UV-induced pyrimidine dimers from the DNA of Chinese hamster cells of the CHO line].
Using DNA sedimentation in alkaline sucrose gradients, the excision of pyrimidine dimers from DNA of CHO cells with the help of UV-endonuclease from Micrococcus luteus has been studied. A considerable proportion (approximately 80%) of pyrimidine dimers was shown to be excised within 26 hours, whereas during the initial 17 hours after the UV-fluence (4 J/M2) the excision was slight (approximately 15%).
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000032:analysis / Q000528:radiation effects*; D004706:Endodeoxyribonucleases; D005260:Female; D006367:HeLa Cells / Q000528:radiation effects; D006801:Humans; D008837:Micrococcus / Q000201:enzymology; D009097:Multienzyme Complexes / Q000494:pharmacology; D009699:N-Glycosyl Hydrolases; D010053:Ovary / Q000166:cytology / Q000528:radiation effects*; D011740:Pyrimidine Dimers / Q000032:analysis / Q000528:radiation effects*; D014466:Ultraviolet Rays
D009097:Multienzyme Complexes; D011740:Pyrimidine Dimers; C032219:UV endonuclease; D004247:DNA; D004706:Endodeoxyribonucleases; D009699:N-Glycosyl Hydrolases
Vyshcheplenie UF-indutsirovannykh pirimidinovykh dimerov iz DNK kletok kitaĭskogo khomiachka linii CHO.