Evidence for a membrane sulfhydryl associated with resistance to melphalan in a murine L1210 leukemia line.
Murine L1210 leukemia cells, which display sensitivity or resistance to the chemotherapeutic alkylating agent, melphalan, are equivalently sensitive or resistant to the poorly permeable mercurial, p-chloromercuribenzenesulfonate. Cells of both lines do not differ in sensitivity to the sulfhydryl reagents, N-ethylamaleimide, iodoacetamide and iodoacetate or to the glutathione transferase substrates 1-chloro-2,4-dinitrobenzene and p-nitrobenzyl chloride. The results are interpreted in terms of the known biological reactivity of p-chloromercuribenzenesulfonate as a selective reagent for sulfhydryl groups of membrane proteins associated with monovalent cation permeability.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004351:Drug Resistance; D007939:Leukemia L1210 / Q000378:metabolism*; D008558:Melphalan / Q000494:pharmacology*; D051379:Mice; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; D008558:Melphalan