Units of chromosome replication and packing.
Fusion between mitotic and S-phase cells induces the formation of prematurely condensed chromosomes (PCC) in the interphase partner. Viewed in the light microscope, S-phase PCC derived from the Indian muntjac appear to be fragmented and heterogeneous. In scanning electron micrographs prepared by an osmium impregnation technique, which avoids the need to sputter-coat the specimen, the S-phase fragments derived from an individual cell are resolved into about 1000 fibre aggregates, together with more dispersed fibres. Aggregates are roughly spherical and vary in diameter between about 0.25 and 1.6 micron. The spatial distribution of the aggregates shows some order: chains of single aggregates and, less commonly, duplicated chains occur. Regions of the PCC where the fibres are more dispersed are considered to be likely candidates for sites of replication at the time of fusion. The relationship between the condensed aggregate structure of the S-phase PCC and replication clusters is discussed, and also the assembly of aggregates to form metaphase chromosomes.
D000818:Animals; D002459:Cell Fusion; D002875:Chromosomes / Q000648:ultrastructure*; D003670:Deer / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007399:Interphase; D008677:Metaphase; D008855:Microscopy, Electron, Scanning; D008938:Mitosis