CDS-AG medium for red blood cell preservation.
Buffy coat-free red cell concentrates (RCC) from ACD, ACD-AG, and EDTA blood were prepared at the day of collection and resuspended with about one half of the packed red cell volume of a citrate-dextrose-sucrose-adenine-guanosine solution (CDS-AG). The posttransfusion survival of RBC in red cell suspensions (RCS) amounted to about 80% after 35 days of storage. 50 to 63% of the normal ATP level still remained. About 0.3% of red cells were hemolysed. 10% of red cells existed unchanged as discocytes, about 85% were transformed to echinocytes and 5% to spherocytes. The MCHC increased by about 25% due to a shrinkage of RBC. A much more hypertonic citrate-saline anticoagulant solution (420 mosmol/kg) increased the shrinkage, led to a higher hemolysis and lower posttransfusion survival rates.
D000225:Adenine; D000925:Anticoagulants; D001793:Blood Preservation / Q000379:methods*; D001803:Blood Transfusion; D002470:Cell Survival; D002951:Citrates; D004912:Erythrocytes / Q000502:physiology*; D005947:Glucose; D006151:Guanosine; D006801:Humans; D006982:Hypertonic Solutions; D013395:Sucrose
D000925:Anticoagulants; D002951:Citrates; D006982:Hypertonic Solutions; D006151:Guanosine; D013395:Sucrose; D005947:Glucose; D000225:Adenine