High pO2-activated inhibitor of protein synthesis in rabbit reticulocytes: its relationship to glutathione disulfide-induced inhibitor and to a approximately 23,000-Mr sulfhydryl protein.
The treatment of reticulocyte post-ribosomal supernatant containing ribosome wash with high pO2 or glutathione disulfide resulted in the activation of an inhibitor of protein synthesis of approximately 23,000-Mr as implicated by its elution from Sephadex G-100. This inhibitor could also be directly activated by exposure of the approximately 23,000-Mr fractions of the control eluate to high pO2 or glutathione disulfide. The high pO2-dependent activation of the inhibitor was blocked by the presence of glucose-6-phosphate or cAMP (2 mM). The inhibitor was stable (and activable) during a 5 minute incubation at 80 degrees C. The analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the G-100 (approximately 23,000-Mr) fractions treated with [14C]N-ethylmaleimide revealed the abolishment of the label in a approximately 23,000-Mr protein band in parallel to high pO2-dependent inhibitor activation.
D000818:Animals; D005033:Ethylmaleimide / Q000378:metabolism; D005978:Glutathione / Q000031:analogs & derivatives / Q000494:pharmacology; D019803:Glutathione Disulfide; D006358:Hot Temperature; D010100:Oxygen / Q000097:blood*; D010313:Partial Pressure; D014176:Protein Biosynthesis / Q000187:drug effects; D011506:Proteins / Q000037:antagonists & inhibitors / Q000302:isolation & purification; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000302:isolation & purification / Q000378:metabolism
D011506:Proteins; D013438:Sulfhydryl Compounds; D005978:Glutathione; D005033:Ethylmaleimide; D010100:Oxygen; D019803:Glutathione Disulfide