Pyridine nucleotide synthesis in human blood--effect of leucine, alpha-ketoisocaproic acid and ketone bodies.
The effects of L-leucine and its metabolites on pyridine nucleotide synthesis were investigated in human blood. After 30 min-incubation 40% of the added (carboxyl-14C)-nicotinic acid was incorporated into pyridine nucleotides, most of which was nicotinic acid mononucleotide. Leucine and alpha-ketoisocaproic acid reduced pyridine nucleotide synthesis by 40% and 15%, respectively at 1 mM level. Ketone bodies were without effect. The results were different from those observed in rat hepatocytes [O. Yamada et al.: Internat. J. Vitam. Nutr. Res. 53, 184 (1983)], in which ketone bodies and the compounds metabolized to ketone bodies were inhibitory to the NAD biosynthesis.
D000227:Adenine Nucleotides / Q000096:biosynthesis / Q000097:blood*; D006801:Humans; D007651:Keto Acids / Q000494:pharmacology*; D007657:Ketone Bodies / Q000494:pharmacology*; D007930:Leucine / Q000494:pharmacology*; D009243:NAD / Q000096:biosynthesis / Q000097:blood; D009249:NADP / Q000097:blood; D009525:Niacin / Q000378:metabolism; D009537:Nicotinamide Mononucleotide / Q000097:blood*
D000227:Adenine Nucleotides; D007651:Keto Acids; D007657:Ketone Bodies; D009243:NAD; D009537:Nicotinamide Mononucleotide; D009525:Niacin; D009249:NADP; C013082:alpha-ketoisocaproic acid; D007930:Leucine