Changes in nuclear translocation of estradiol-receptor complex in anterior pituitary and uterus of rats with streptozotocin diabetes.
We have studied estradiol (E2) receptor nuclear translocation in anterior pituitary and uterus of ovariectomized control and diabetic rats one month after diabetes induction with Streptozotocin. Animals were pretreated with a low (0.5 microgram/100 g) or a high (25 micrograms/100 g) E2 dose 60 min before killing for the pituitary nuclear translocation. We observed that with the low E2 dose, nuclear translocation was reduced in pituitary from diabetic rats; the low dose given for 4 days also resulted in reduced induction of cytosolic progestin receptors in the pituitary and lower serum prolactin response in the diabetic group. With the 25 micrograms/100 g E2 dose, E2-receptor translocation, and the biological activity of E2 (induction of progestin receptors and serum prolactin response) were in the normal range. Serum E2 in controls and diabetics treated with E2 were not different. In the uterus, both low and high E2 doses given for 4 days resulted in significantly reduced nuclear translocation and uterine weight increment in the diabetic animals. These results suggest a brain-pituitary disturbance in addition to a peripheral (uterine) relative insensitivity to sex hormones as contributing factors to the reproductive failure of diabetic animals.
D000818:Animals; D002369:Castration; D002467:Cell Nucleus / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D010903:Pituitary Gland, Anterior / Q000648:ultrastructure*; D011388:Prolactin / Q000097:blood; D051381:Rats; D011919:Rats, Inbred Strains; D011959:Receptors, Estradiol; D011960:Receptors, Estrogen / Q000187:drug effects / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism; D014599:Uterus / Q000648:ultrastructure*
D011959:Receptors, Estradiol; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D004958:Estradiol; D011388:Prolactin