A simple automated injection technique for the high-pressure liquid chromatographic determination of plasma retinol.
We describe a rapid automated method for determination of retinol in 100 microliters plasma. A lipid extract of plasma is analysed by reverse phase high pressure liquid chromatography using methanol-water as eluent. Automation of injection is achieved by adaptation of a Technicon AutoAnalyzer Sampler 2 coupled with a Micromedic dilution system and a pneumatically actuated loop injector. Twenty patients' specimens can be analysed in 4 hours, including extraction time, standards, and quality controls, with one extract injected every 4 minutes.
D000328:Adult; D000368:Aged; D000375:Aging; D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002940:Circadian Rhythm; D005260:Female; D006801:Humans; D008297:Male; D014801:Vitamin A / Q000097:blood*
D014801:Vitamin A