Effects of polyene cholesterol-binding antibiotics on the binding and removal of cholesterol from bovine adrenocortical cytochrome P-450SCC: difference in properties of the membrane-bound and membrane-free cytochrome.
The polyene cholesterol-binding antibiotics filipin and amphotericin B inhibited the binding of cholesterol to steroid-free, soluble adrenocortical cytochrome P-450SCC. In mitochondria-bound cytochrome P-450SCC, cholesterol was not extracted by filipin, whereas filipin was able to react with cholesterol bound to purified soluble cytochrome P-450SCC. Based on these results we conclude that the cholesterol-binding site of membrane-bound cytochrome P-450SCC is masked by membrane structures.
D000302:Adrenal Cortex / Q000378:metabolism*; D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002784:Cholesterol / Q000502:physiology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005372:Filipin / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D011090:Polyenes / Q000494:pharmacology*; D011485:Protein Binding
D011090:Polyenes; D000666:Amphotericin B; D005372:Filipin; D003577:Cytochrome P-450 Enzyme System; D002784:Cholesterol