Activation of complement in C-reactive protein positive sera by phosphorylcholine-bearing component isolated from parasite extract.
Phosphorylcholine-bearing component levels in extracts of various parasites were determined by a capillary precipitin test using anti-phosphorylcholine Ig A myeloma protein. TEPC-15. Phosphorylcholine was demonstrated as a structural component not only in nematodes but also in trematodes and cestodes. The phosphorylcholine-bearing component was isolated from an extract of Toxocara canis larvae using a TEPC-15-Sepharose 4B column. The component reacted with C-reactive protein in sera to form one precipitin line in immunoelectrophoresis. The component provided two Brilliant Coomassie Blue positive bands in SDS-polyacrylamide gel electrophoresis. It reacted with C-reactive protein to activate complement in serum.
D000818:Animals; D001199:Ascaridoidea / Q000276:immunology; D002097:C-Reactive Protein / Q000276:immunology*; D002794:Choline / Q000031:analogs & derivatives*; D003167:Complement Activation; D004170:Diphyllobothrium / Q000276:immunology; D006801:Humans; D010238:Paragonimus / Q000276:immunology; D010767:Phosphorylcholine / Q000276:immunology* / Q000302:isolation & purification; D014119:Toxocara / Q000032:analysis / Q000276:immunology*; D014234:Trichinella / Q000276:immunology
D010767:Phosphorylcholine; D002097:C-Reactive Protein; D002794:Choline