[Growth characteristics of haploid and diploid Hansenula polymorpha yeasts on methanol in continuous culture].
The rate of growth and the yield of biomass of a Hunsenula polymorpha homozygous diploid strain ML-3 X ML-3 were nearly identical with those of the parent haploid culture ML-3. The two cultures were eliminated from the fermenter at D = 0.20 to 0.21 h-1. In contrast, a heterozygous diploid strain ML-3 X VKM 1397 could grow at D = 0.23 to 0.25 h-1 and its biomass yield reached 38% while the yields of the haploid and homozygous diploid strains were 35 to 36%. The pH of the medium had the same effect on the three cultures: a change in the pH from 4.0 to 3.5 and then to 3.0 did not influence the yield of biomass; a further pH drop to 2.5 made the cells be eliminated from the fermenter.
D001203:Ascomycota / Q000254:growth & development*; D003433:Crosses, Genetic; D004171:Diploidy; D005285:Fermentation; D006238:Haploidy; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000378:metabolism*; D010843:Pichia / Q000254:growth & development* / Q000378:metabolism
D000432:Methanol
Kharakteristika rosta gaploidnykh i diploidnykh drozhzheĭ Hansenula polymorpha na metanole v usloviiakh nepreryvnogo kul'tivirovaniia.