Neuropeptide Y in neuroblastoma X glioma hybrid cells. Response to dexamethasone and nerve growth factor.
High concentrations of a newly-identified biologically potent peptide, neuropeptide Y, have been demonstrated in 3 related mouse neuroblastoma-derived clonal cell lines, N18TG2 0.35 pmol/mg protein, NG108-15 0.44 pmol/mg protein and NCB-20 0.39 pmol/mg protein. The NG108-15 cell line was chosen for further evaluation. Dexamethasone (10 microM) and nerve growth factor (10 ng/ml) resulted in a 2-fold increase in cellular neuropeptide Y concentrations. The response to dexamethasone was demonstrated to be dose-dependent. Exposure to both agents in combination resulted in a more than additive effect, indicating synergism.
D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003907:Dexamethasone / Q000494:pharmacology*; D005910:Glioma / Q000503:physiopathology*; D006822:Hybrid Cells / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D051379:Mice; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D009447:Neuroblastoma / Q000503:physiopathology*; D009478:Neuropeptide Y; D051381:Rats
D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D003907:Dexamethasone