Interaction of hapten-sensitized liposomes with cells bearing hapten-specific receptors.
The binding of liposomes sensitized with 2,4-dinitrophenyl-6-N-aminocaproylphosphatidylethanolamine (DNP-Cap-PE) to MOPC-315 cells, which secrete and bear on their surfaces anti-DNP immunoglobulins, was studied. The binding was affected by cholesterol content, phospholipid composition and hapten density of liposomes: The binding of distearoylphosphatidylcholine liposomes sensitized with 5 mol% hapten to the cells increased with increasing cholesterol content in liposomes. The amount of liposomes composed of phospholipid with a higher transition temperature (such as distearoylphosphatidylcholine), which bound to MOPC-315 cells, was much higher than that of liposomes composed of phospholipid with a lower transition temperature (such as egg yolk phosphatidylcholine). The amount of distearoylphosphatidylcholine liposomes containing equimolar cholesterol, which bound to the cells at 0 degrees C, increased with increasing amount of the hapten in liposomes up to 2.5 mol%. The binding became maximum at 2.5 mol% and decreased with higher hapten density in liposomes. The immunogenicity of hapten-sensitized liposomes is known to be affected by the liposomal composition such as cholesterol content, phospholipid composition and hapten density. This model study suggests that the binding of liposomes to cells is important for expressing the immunogenicity of hapten-sensitized liposomes.
D000818:Animals; D002460:Cell Line; D002784:Cholesterol / Q000494:pharmacology; D006241:Haptens; D007700:Kinetics; D008081:Liposomes; D051379:Mice; D010714:Phosphatidylethanolamines; D010743:Phospholipids / Q000494:pharmacology; D010954:Plasmacytoma / Q000276:immunology; D011946:Receptors, Antigen / Q000187:drug effects / Q000276:immunology*
C021015:2,4-dinitrophenyl-6-N-aminocaproylphosphatidylethanolamine; D006241:Haptens; D008081:Liposomes; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D011946:Receptors, Antigen; D002784:Cholesterol