Radioimmunoassay of testosterone-estradiol-binding globulin in humans: a reassessment of normal values.
Antiserum against human testosterone-estradiol-binding globulin (hTeBG) was prepared in rabbits, and its specificity was demonstrated by crossed immunoelectrophoresis. A RIA for the measurement of hTeBG in serum was developed. With this assay, hTeBG was readily measured in 1-2 microliters serum. Interassay coefficients of variation for pools of serum from men, women, and women in late pregnancy were 7.7%, 5.5%, and 6.1%, respectively. Interassay coefficients of variation for the same pools were 10%, 12%, and 12%, respectively. The TeBG levels in a number of nonselected subjected determined by the present method show good correlations with those obtained by steady state polyacrylamide gel electrophoresis and dextran-coated charcoal assay. The concentrations of TeBG determined by the RIA in sera from men, women, and women in late pregnancy were 18 +/- 9 (n = 12), 54 +/- 13 (n = 8), and 374 +/- 55 (n = 6) pmol/ml (mean +/- SD), respectively.
D000653:Amniotic Fluid / Q000032:analysis; D000818:Animals; D004355:Drug Stability; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007457:Iodine Radioisotopes; D008253:Macaca mulatta; D008297:Male; D011247:Pregnancy; D011863:Radioimmunoassay / Q000592:standards; D012015:Reference Standards; D012016:Reference Values; D012453:Saimiri; D012738:Sex Hormone-Binding Globulin / Q000032:analysis* / Q000276:immunology
D007106:Immune Sera; D007457:Iodine Radioisotopes; D012738:Sex Hormone-Binding Globulin