Calorimetric techniques for metabolic studies of cells and organisms under normal conditions and stress.
When cells are subjected to stress, an early result is a shift in type and rate of metabolism to reflect their new conditions. The availability of metabolites, their endogenous vs exogenous origins, and the rates at which they can be used, besides availability of oxygen, dictate cell and tissue response. Measurement of heat output in such a response is a means for monitoring cells and tissues. Differential heat conduction calorimeters are reviewed to provide a listing of instrument parameters important in optimum practical use. Data obtained with one cell system, mammalian sperm, are presented to provide an example of how the combination of calorimetry and carbon balance, plus calculation from thermodynamic constants, permit an assessment of the importance of endogenous metabolism to total cellular metabolism.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001419:Bacteria / Q000378:metabolism; D002151:Calorimetry / Q000295:instrumentation / Q000379:methods*; D002417:Cattle; D002477:Cells / Q000378:metabolism*; D066298:In Vitro Techniques; D007448:Invertebrates / Q000378:metabolism; D008297:Male; D008660:Metabolism; D013094:Spermatozoa / Q000378:metabolism; D013816:Thermodynamics
D000255:Adenosine Triphosphate