title: Ultrastructure of normal and torn menisci of the human knee joint.
abstract: Normal human menisci obtained at autopsy (seven cases) and the injured and uninjured portions of torn menisci obtained at surgery (nine cases) were studied with the electron microscope. The surface of menisci is composed of collagen fibrils surmounted by an electron-dense surface coat. Most of the cells in menisci are chondrocytes but a few fibroblasts and cells of an intermediate form difficult to classify as either fibroblasts or chondrocytes also occur. Mast cells are found at the vascularised periphery of the meniscus. Myofibroblasts were found in the injured portions of menisci in three out of the nine cases studied. A territorial matrix containing fibrils and proteoglycan particles with associated filaments is seen around or adjacent to chondrocytes, but sometimes this matrix is sparse or absent. The interterritorial or general matrix comprises collagen fibrils of widely varying diameters (25-180 nm) set in a sparse interfibrillary matrix containing proteoglycan particles. A few mature elastic fibres and several small or immature elastic fibres and collections of electron-dense filaments are seen in the general matrix. Also seen in this region are calcified bodies and matrical lipidic debris derived by the shedding of cell processes and in situ necrosis of cells. Other features seen in the matrix of the injured portion of the meniscus include: (1) membrane-bound cystic structures; (2) parting and fraying of collagen fibrils; and (3) pools of proteoglycan particles.
mesh_terms: D000293:Adolescent; D000328:Adult; D002470:Cell Survival; D003094:Collagen / Q000032:analysis; D005260:Female; D005347:Fibroblasts / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000648:ultrastructure; D008592:Menisci, Tibial / Q000032:analysis / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008875:Middle Aged; D000070600:Tibial Meniscus Injuries
chemical_list: D003094:Collagen