Polyamide resin: a novel immunoadsorbent. I. Preparation of polyamide resin-antigen conjugates for use in a solid phase assay for radiolabeled antibody synthesized in vitro.
A solid phase assay for radiolabeled antibody synthesized de novo in vitro has been described (1). The solid phase consists of antigen covalently bound to bromacetyl cellulose, a useful but difficult to prepare immunoadsorbent. Herein, we describe the preparation of polyamide resin immunoadsorbent and the procedure for coupling antigen to the polymer. Data are presented that show that polyamide resin-Ag conjugates can replace bromacetyl cellulose-Ag conjugates. The usefulness of this easily prepared and inexpensive immunoadsorbent is discussed.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000936:Antigen-Antibody Complex / Q000032:analysis; D000941:Antigens; D007106:Immune Sera / Q000032:analysis; D007164:Immunosorbents; D009757:Nylons; D011817:Rabbits / Q000276:immunology; D012117:Resins, Synthetic
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000941:Antigens; D007106:Immune Sera; D007164:Immunosorbents; D009757:Nylons; D012117:Resins, Synthetic