[Neurons of the enteral portion of the metasympathetic system cultured in diffusion chambers].
A culture of the neurons of metasympathetic system's enteral portion of 3-5-day old rats was obtained and fragments of the mienteral plexus were implanted to adult rats-recipients. A technique of cultivation was developed for in vivo microscopy of culture's neurons. Migration of neuroblasts from the deplantate area was revealed as well as the specific features of neurons differentiation. The neurons were shown to acquire characteristic morphological features corresponding to Dogel's I and II types of neurons; growth of neurites was shown to aid to formation of neuropil and microglia. Histochemical analysis revealed a product of response to cholinesterase which is the index of formation of the acetylcholine hydrolysis system in the culture's I type neurons. The technique of electron-microscopic scanning enables to watch in detail the process of formation of interneuronal connections and formation of a synapse.
D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D002465:Cell Movement; D003106:Colon / Q000294:innervation*; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods; D007365:Intercellular Junctions / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000648:ultrastructure*; D051381:Rats; D013564:Sympathetic Nervous System / Q000648:ultrastructure*
Issledovanie neĭronov énteral'noĭ chasti metasimpaticheskoĭ sistemy pri kul'tivirovanii v diffuzionnykh kamerakh.