An essential role for glucocorticoid in casein gene expression in rat mammary explants.
It is demonstrated that the accumulation of 42 K casein mRNA in mammary tissue from adrenalectomized, virgin rats is almost 20x higher in the presence of exogenous hydrocortisone than in its absence. Accumulation of 25 K casein mRNA in this tissue is totally dependent on the steroid. The results indicate a much greater dependency on hydrocortisone than was appreciated previously, and also show that this dependency does not reflect a loss of cell viability in the absence of the steroid.
D000315:Adrenalectomy; D000818:Animals; D002364:Caseins / Q000235:genetics*; D004247:DNA / Q000378:metabolism; D005260:Female; D005796:Genes / Q000187:drug effects*; D006854:Hydrocortisone / Q000494:pharmacology*; D007700:Kinetics; D008321:Mammary Glands, Animal / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D009924:Organ Culture Techniques; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic / Q000187:drug effects*
D002364:Caseins; D012333:RNA, Messenger; D004247:DNA; D006854:Hydrocortisone