Effects of emetine on initiation of DNA synthesis in embryonic cells of sea urchin.
The effects of the inhibition of protein synthesis by emetine on the initiation of DNA synthesis and mitosis in the fertilized eggs of the sea urchin, Anthocidaris crassispina, were studied. The initiation of DNA synthesis was completely prevented, and no delayed initiation was observed in eggs in which protein synthesis was completely inhibited by emetine, while delayed initiation of DNA synthesis occurred after prolonged culture in eggs in which protein synthesis was incompletely inhibited by emetine. In both cases, neither mitosis nor cleavage was observed. Synthesis of some protein factor seems to be necessary for the initiation of DNA synthesis.
D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D004640:Emetine / Q000494:pharmacology*; D005260:Female; D008938:Mitosis / Q000187:drug effects; D014176:Protein Biosynthesis; D012617:Sea Urchins / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D015053:Zygote / Q000187:drug effects / Q000378:metabolism*
D004247:DNA; D004640:Emetine