Characteristics of the cytoplasmic and nuclear dihydrotestosterone receptors of human thymic tissue.
Dihydrotestosterone receptor was prepared from both the cytoplasm and nucleus of human thymic tissue. Human cytoplasmic and nuclear dihydrotestosterone receptor possessed a KA greater than 1.0 and by competition assay was highly specific for dihydrotestosterone. Human thymic cytoplasmic dihydrotestosterone receptor prepared in standard buffer sedimented on 5-20% sucrose gradients in the 4s region but human thymic cytoplasmic dihydrotestosterone receptor prepared in buffer containing sodium molybdate (10 mM) sedimented in the 7s region on 5-20% sucrose gradients. It is concluded that the human thymic dihydrotestosterone receptor is similar to classic androgen receptor.
D000293:Adolescent; D000328:Adult; D002467:Cell Nucleus / Q000378:metabolism*; D002499:Centrifugation, Density Gradient / Q000379:methods; D002648:Child; D002675:Child, Preschool; D003600:Cytosol / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism*; D005260:Female; D006801:Humans; D007223:Infant; D007700:Kinetics; D008297:Male; D011944:Receptors, Androgen / Q000302:isolation & purification / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D012737:Sex Factors; D013950:Thymus Gland / Q000378:metabolism*
D011944:Receptors, Androgen; D011987:Receptors, Steroid; D013196:Dihydrotestosterone