Double Y as an indicator in a test of mitotic nondisjunction in cultured human lymphocytes.
A cytological test for the detection of non-disjunction in human cultured lymphocytes has been developed. The Y chromosome was used as a marker, because this chromosome is easily identifiable in mitosis after staining with quinacrine dihydrochloride without karyotyping. Non-disjunction can be directly revealed by scoring mitoses with two Ys. The test was applied to agents known for their ability to induce numerical and/or structural changes in chromosomes, namely benomyl, colcemid, distamycin A, mitomycin C and X-rays. In treated lymphocytes, increased frequencies of YY mitoses were found, ranging from 2.5 X 10(-4) to 61.2 X 10(-4) (control value less than or equal to 1.1 X 10(-4). The results suggest that the detection of fluorescent Y chromosomes on metaphases is suitable for determining induced non-disjunction in human cultured cells.
D002478:Cells, Cultured; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D008297:Male; D008856:Microscopy, Fluorescence; D008938:Mitosis / Q000187:drug effects / Q000528:radiation effects; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009630:Nondisjunction, Genetic; D012730:Sex Chromosomes / Q000502:physiology*; D014998:Y Chromosome / Q000502:physiology*
D009153:Mutagens