A radiotechnique suitable for the detection of octopine synthesis in crown-gall tissues grown in vitro.
An improved method for the detection of octopine in Crown-gall tissues and in shoots regenerated from these tissues has been devised using [14C]arginine as a precursor. By using bidimensional chromatography followed by autoradiography, very small amounts of octopine can be clearly detected in tumors grown in vitro. Using this method, octopine was detected in tumor tissues when usual techniques were not sensitive enough.
D001120:Arginine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D002250:Carbon Radioisotopes; D002999:Clone Cells; D007553:Isotope Labeling; D010941:Plant Tumors / Q000032:analysis*
D002250:Carbon Radioisotopes; C005394:octopine; D001120:Arginine