F-actin aggregates may activate transformed cell surfaces.
Observations on the role of transformation-specific F-actin aggregates [Carley et al, 1981] in altering morphology, adhesion and intercellular interaction in transformed cells are reported here. The appearance and disappearance of membrane- and substrate-associated F-actin aggregates (MAG and SAG, respectively) are followed in a cell line temperature-sensitive for transformation. Since MAG structures also appear near the membrane in suspension cultures of transformed cells and in transformed cells in coculture with untransformed cells, they appear to function at cell-cell contacts. Unlike microfilament bundles in untransformed cells, MAG and SAG do not contain the F-actin regulatory protein tropomyosin. The lack of tropomyosin in these structures near the membrane is reminiscent of areas of an exceptionally active actin cytoskeleton usually associated with motile processes of the normal cell membrane. Such areas of membrane-cytoskeletal interaction may be involved in the aberrant cell-cell communication as well as the aggressive behavior often seen in transformed cells.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002450:Cell Communication; D002462:Cell Membrane / Q000378:metabolism; D002472:Cell Transformation, Viral; D007668:Kidney / Q000473:pathology; D051381:Rats; D013696:Temperature
D000199:Actins