title: Immunoassay of unconjugated estriol in serum of pregnant women monitored by chemiluminescence.
abstract: 6-Oxoestriol-6-(O-carboxymethyl)oxime-aminobutylethyl- isoluminol conjugate was synthesized. This luminogenic estriol derivative enabled us to develop a solid phase immunoassay method for the determination of unconjugated estriol in serum of pregnant women by the measurement of the bound estriol-isoluminol conjugate upon oxidation with a hydrogen peroxide/microperoxidase system. The sensitivity of the assay was 700 pmol/l. Results obtained by radioimmunoassay and the described method showed good agreement (r = 0.95). The chemiluminescent method is applicable in the routine measurement of unconjugated estriol.
mesh_terms: D004964:Estriol / Q000031:analogs & derivatives / Q000097:blood* / Q000138:chemical synthesis; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements; D011247:Pregnancy; D011863:Radioimmunoassay
chemical_list: C032321:estriol-16alpha-glucuronyl-6-aminobutyl-ethyl-isoluminol; D004964:Estriol