title: High concentrations of dimethylamine and methylamine in squid and octopus and their implications in tumour aetiology.
abstract: The levels of the common secondary amines in various squid, in octopus and in 17 other seafoods were determined by HPLC. Ammonia and dimethylamine were found in all of the seafoods tested and some of them also contained methylamine and/or ethylamine. Particularly high levels of dimethylamine (946-2043 ppm) and methylamine (38-255 ppm) were detected in various species of squid and in the octopus. Reaction of nitrite in acidic medium with aqueous extract of squid yielded appreciable amounts of N-nitrosodimethylamine. The optimum pH for this reaction was around 2.4. Dimethylamine in dried squid tissues was readily extracted with water or 1% sodium carbonate solution. Heat treatment of dried squid at 200 degrees C was found to increase its amine content dramatically. It appeared that pyrolytic decarboxylation of some amino acids might cause this increase. Squid is a popular seafood in Japan and other oriental countries. The high incidence of stomach cancer in Japan and China is thought by epidemiologists to be associated with traditional Japanese and Chinese diets. Our present finding that squid and other seafoods contain unusually high levels of dimethylamine and other amines adds to the evidence that dietary factors may have an important role in the aetiology of stomach cancer and other gastro-intestinal tumours.
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D049832:Decapodiformes / Q000032:analysis*; D004032:Diet / Q000009:adverse effects; D004123:Dimethylamines / Q000032:analysis*; D004128:Dimethylnitrosamine / Q000032:analysis; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000209:etiology*; D006801:Humans; D007564:Japan; D008297:Male; D008744:Methylamines / Q000032:analysis*; D049831:Octopodiformes / Q000032:analysis*
chemical_list: D004123:Dimethylamines; D008744:Methylamines; D004128:Dimethylnitrosamine