Identification of exposed components on the surface of adult Schistosoma mansoni by lactoperoxidase-catalysed iodination.
Adult schistosomes have been labelled with 125I using the lactoperoxidase-catalysed technique modified to cause minimal worm damage. After surface membrane removal and characterization, at least 13 labelled proteins were identified together with a large amount of labelled glycolipids, free fatty acids and phospholipids, especially phosphatidyl ethanolamine. Cationised ferritin has been used to stimulate surface membrane turnover of iodinated worms and the shedding of covalently bound 125I-counts used as an index of turnover. Finally worms have been iodinated before and after stimulation of membrane turnover in chemically defined media and the patterns of labelled proteins were compared.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D002384:Catalysis; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D007457:Iodine Radioisotopes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007784:Lactoperoxidase; D008055:Lipids / Q000032:analysis; D010544:Peroxidases; D011506:Proteins / Q000378:metabolism; D012550:Schistosoma mansoni / Q000648:ultrastructure*
D007457:Iodine Radioisotopes; D008055:Lipids; D011506:Proteins; D007770:L-Lactate Dehydrogenase; D007784:Lactoperoxidase; D010544:Peroxidases; D000469:Alkaline Phosphatase