HeLa nuclear, mitochondrial and microsomal binding proteins for vitamin A compounds.
HeLa cells cultured in Eagle's medium incorporate and metabolize (3H) 11,12-retinyl acetate: TLC analysis of vitamin A extracts shows that no more than 7% of retinyl acetate is recovered from the cell sap, whole homogenate, nuclei, mitochondria and microsomes. Physiological active vitamin A compounds (retinol, retinal and retinoic acid) represent about 65% of the vitamin A extracted from homogenate, nuclei, mitochondria and microsomes. 1% SDS extracts of nuclei and mitochondria were analyzed by PAG electrophoresis. Nuclear electrophoregrams show vitamin A radioactivity in three zones of the gel corresponding to proteins with molecular weights of 15,000; 19,000 and 23,000 respectively. In mitochondrial electrophoregrams vitamin A radioactivity is located in three zones of the gel, corresponding to proteins with molecular weights of 16,500, 24,000 and 85,000 respectively.
D002470:Cell Survival / Q000187:drug effects; D004224:Diterpenes; D006367:HeLa Cells / Q000032:analysis*; D006801:Humans; D008854:Microscopy, Electron; D008861:Microsomes / Q000032:analysis; D008928:Mitochondria / Q000032:analysis; D008970:Molecular Weight; D012177:Retinol-Binding Proteins / Q000032:analysis*; D000084562:Retinyl Esters; D014801:Vitamin A / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology
D004224:Diterpenes; D012177:Retinol-Binding Proteins; D000084562:Retinyl Esters; D014801:Vitamin A; C009166:retinol acetate