DNA-binding domain in adenovirus core protein VII.
Major core protein, VII of adenovirus type 2 can be cleaved with BrCN into four fragments A, B, C and D according to the electrophoretic mobility on SDS-polyacrylamide gel. The sequential order of the fragments was ACBD from NH2-terminus to COOH-end. From the results of protein blotting experiment and nitrocellulose filter binding assay, fragments A and C, the amino end half of VII, was suggested to be DNA-binding domain. Furthermore, the binding of VII to DNA is suggested to become tighter as the fragment A moiety of pVII is processed in the virion.
D000260:Adenoviruses, Human / Q000032:analysis*; D002277:Carcinoma; D002460:Cell Line; D003488:Cyanogen Bromide; D004247:DNA; D006801:Humans; D009062:Mouth Neoplasms; D010446:Peptide Fragments / Q000032:analysis; D011485:Protein Binding; D014758:Viral Core Proteins; D014764:Viral Proteins / Q000302:isolation & purification*
D010446:Peptide Fragments; D014758:Viral Core Proteins; D014764:Viral Proteins; D004247:DNA; D003488:Cyanogen Bromide