A modified form of mitochondrial hexokinase produced by ATP-induced solubilization.
Mitochondrial hexokinases from several rat tissues were analyzed by DEAE-cellulose chromatography. Solubilization by glucose-6-P or Triton X-100 released hexokinases A and B. Solubilization by ATP resulted in a decrease of hexokinase A and the concomitant appearance of a new fraction of lower net charge (hexokinase Am) which readily reverts to hexokinase A by dialysis or dilution. Treatment of homogeneous or partially purified hexokinase A with ATP did not generate hexokinase Am. Hexokinases Am and A were equally inhibited by an anti-hexokinase immune serum and displayed the same Km values for glucose and ATP. Hexokinase Am may represent a conformer or an oligomer produced during ATP-induced solubilization of hexokinase A from mitochondria.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000201:enzymology; D002848:Chromatography, DEAE-Cellulose; D003902:Detergents; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates; D006593:Hexokinase / Q000302:isolation & purification* / Q000378:metabolism; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology; D017830:Octoxynol; D011092:Polyethylene Glycols; D051381:Rats; D011915:Rats, Inbred BUF; D011919:Rats, Inbred Strains; D012995:Solubility
D003902:Detergents; D005958:Glucosephosphates; D007527:Isoenzymes; D011092:Polyethylene Glycols; D019298:Glucose-6-Phosphate; D000255:Adenosine Triphosphate; D017830:Octoxynol; D006593:Hexokinase