A rapid separation of S100 subunits by high performance liquid chromatography: the subunit compositions of S100 proteins.
The subunits of S100 protein were dissociated and separated from each other by high performance liquid chromatography on a macroreticular poly-styrene resin, using an acetonitrile-trifluoroacetic acid solvent system. The separation was completed within 80 min in monitoring the effluent at 210 and 280 nm, allowing a rapid and sensitive identification of each S100 protein in terms of subunit composition, and a simultaneous purification of subunits. The method enabled to estimate the subunit compositions of S100a, S100b, and S100a0 protein purified from bovine brain, and revealed a micro-heterogeneity of S100 subunits as applied to the mixtures of S100 protein prepared from bovine, human and rat brain.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015415:Biomarkers; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D009414:Nerve Growth Factors; D051381:Rats; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000302:isolation & purification*; D013045:Species Specificity
D000596:Amino Acids; D015415:Biomarkers; D009414:Nerve Growth Factors; C030564:S-100 calcium-binding protein alpha subunit; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579160:S100B protein, human; C579158:S100b protein, rat