Identification of species-specific and gender-specific proteins and glycoproteins of three human schistosomes.
Species-specific and gender-specific polypeptides of Schistosoma haematobium, Schistosoma japonicum, and Schistosoma mansoni have been identified. Proteins of these schistosomes were metabolically labeled in vitro with 35S-methionine and their total proteins, concanavalin-A binding glycoproteins, released (shed or secreted) proteins, and released glycoproteins compared by two-dimensional polyacrylamide electrophoresis. Many of the released proteins were glycosylated, and most of the synthesized glycoproteins were released. The most striking gender-specific and species-specific differences were observed in the released glycoproteins. These results provide a basis for investigating the molecular evolution of schistosomes, the occurrence of dioecy in the schistosomatidae , and for the development of improved serodiagnostic reagents.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000032:analysis* / Q000096:biosynthesis; D008297:Male; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D012548:Schistosoma haematobium / Q000032:analysis*; D012549:Schistosoma japonicum / Q000032:analysis*; D012550:Schistosoma mansoni / Q000032:analysis*; D012737:Sex Factors; D013045:Species Specificity
D006023:Glycoproteins; D011506:Proteins