Freeze-fracture characterization of the outermost Golgi cisterna (OGC) in rat pancreatic acinar cells.
The outermost Golgi cisterna (OGC) frequently exhibits 55 nm diameter protuberances in P fracture faces and corresponding depressions, or pits, in E faces. When these protuberances (pits) appear regularly disposed, OGC faces are recognizable at relatively low electron microscopic magnifications. The mean particle width is less in OGC (6.4 nm) than in rough ER (7.9 nm) P faces, while particle number per unit area is respectively 70% and 100% greater in OGC P and E faces than in corresponding ER faces. The rather small OGC P face particles are better resolved at a relatively low shadowing angle. These differences in particle size (for P faces) and density between OGC and rough ER faces are detectable by inspection and may be used in the recognition of OGC faces devoid of protuberances (in P faces) and pits (in E faces). Fusion of presumably rough ER-derived microvesicles form short double-bossed tubular elements which constitute the OGC initial structure. These tubules enlarge by addition of microvesicle membrane and contents forming a characteristic sheet-like bossed structure.
D000818:Animals; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005614:Freeze Fracturing; D006056:Golgi Apparatus / Q000648:ultrastructure*; D007425:Intracellular Membranes / Q000648:ultrastructure; D008854:Microscopy, Electron; D010179:Pancreas / Q000648:ultrastructure*; D051381:Rats