title: Mutagenic and DNA damaging activity in muscle of trout exposed in vivo to nitrite.
abstract: Muscle ether extracts of rainbow trout (Salmo gairdneri) exposed to lake water enriched with nitrite (450 micrograms/l) reverted plasmid-containing his- strains of Salmonella typhimurium, mainly eliciting frameshift mutations, and induced a DNA damage in Escherichia coli reparable through the recA/lexA-dependent SOS functions. The number of revertants was related to their content in nitroso-derivatives and to the physiological condition of the fish. Mutagenicity was efficiently decreased, through NADPH-requiring pathways, by liver S-9 fractions from rats or rainbow trout, while it was not affected by preliminary heating nor by pre-incubation with human gastric juice.
mesh_terms: D000818:Animals; D004247:DNA / Q000378:metabolism*; D005618:Fresh Water; D009132:Muscles / Q000378:metabolism*; D009152:Mutagenicity Tests; D009153:Mutagens; D009573:Nitrites / Q000633:toxicity*; D012977:Sodium Nitrite / Q000633:toxicity*; D014020:Tissue Extracts / Q000633:toxicity; D014337:Trout; D014873:Water Pollutants / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D009153:Mutagens; D009573:Nitrites; D014020:Tissue Extracts; D014873:Water Pollutants; D014874:Water Pollutants, Chemical; D004247:DNA; D012977:Sodium Nitrite