Thin-layer fluorescence cell for ligand binding studies.
A high precision method for measuring the binding of gaseous ligands to proteins is presented. Front face fluorescence techniques are utilized with a special thin-layer cell in order to monitor the change in fluorescence intensity caused by changing the ligand partial pressure. The method is illustrated by examining the binding of carbon monoxide to hemocyanin from the lobster Homarus americanus.
D000818:Animals; D002248:Carbon Monoxide; D005740:Gases; D006433:Hemocyanins; D008024:Ligands / Q000032:analysis*; D008121:Nephropidae; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
D005740:Gases; D008024:Ligands; D002248:Carbon Monoxide; D006433:Hemocyanins