[Estradiol stimulation of ribosomal synthesis in adenohypophysis cells].
A single dose of 10 micrograms oestradiol injected to a male rat stimulates in the anterior pituitary the synthesis of ribosomal RNA and of the associated proteins. This stimulation is shown using in vitro double-labeling of RNA with adenine or guanine and of proteins with valine. The analysis of polysomes reveals the incorporation of the neo-synthesized molecules into the 40 S and 60 S subunits. Therefore, the stimulation of ribosomal RNA and protein biosynthesis by oestradiol is a coordinated process. No change in the whole polysome distribution is observed in these conditions though such a modification may occur in a specific cell population without being detected by using sucrose gradient analysis.
D000225:Adenine / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D004958:Estradiol / Q000494:pharmacology*; D006147:Guanine / Q000378:metabolism; D008297:Male; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism*; D014176:Protein Biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium; D014633:Valine / Q000378:metabolism
D002250:Carbon Radioisotopes; D012335:RNA, Ribosomal; D014316:Tritium; D004958:Estradiol; D006147:Guanine; D014633:Valine; D000225:Adenine
Stimulation par l'oestradiol de la synthèse des ribosomes dans les cellules adénohypophysaires.