Ductus thoracicus lymph in mice. 1. A technique for cervical approach.
A method is described for the collection of lymph from the thoracic duct in anaesthetized mice by a silastic catheter inserted into the duct proximal to the jugulo-subclavian junction. The operative procedure takes about 5 min, and there is less operative trauma to the animals than by the previously used abdominal approach. Lymph flow amounts to 0.396 ml/h and cell content to 38.6 X 10(6)/ml.
D000818:Animals; D002404:Catheterization; D008196:Lymph; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013048:Specimen Handling / Q000379:methods*; D013897:Thoracic Duct