Preferential nucleolar localization of Cis-DDP in human fibroblasts.
The use of complex platinum salts (Cis-DDP) in antitumoral therapy is well known. However the mode of action of this salt remains unknown. The present study was performed with human transformed cells in culture (WI-98-VAD), grown in the presence of Cis-DDP. The intracellular localization of platinum was studied by associating the technique of microanalysis by electronic probe (analysis by wavelength dispersion: W. D. S.) and that of fine tissue sections at low temperature: ultracryotomy. The platinum was found to be concentrated mainly in the nucleolar region.
D002466:Cell Nucleolus / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D002945:Cisplatin / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans
D002945:Cisplatin