Ultrastructural changes during the enhancement of cellular 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase in a Chinese hamster cell mutant resistant to compactin (ML 236B).
Chinese hamster V79 cell mutants resistant to compactin (ML236B) were isolated. A resistant clone, MF-2, grown in the presence of 2 micrograms/ml of ML236B for 1 week showed a 30-fold increase in 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG-Co A) reductase activity compared to MF-2 grown in the absence of ML236B, and cells grown for 4 weeks showed a 53-fold increase. Apparent ultrastructural changes in thin sections of the MF-2 cells were observed after growth in ML236B: dilated cisternae in the rough endoplasmic reticulum had or did not have flocculated contents; there was significant distension of perinuclear space; and vesicular inclusion bodies were present in nuclei.
D000818:Animals; D002999:Clone Cells / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D006224:Cricetinae; D003412:Cricetulus; D004351:Drug Resistance; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D008148:Lovastatin / Q000031:analogs & derivatives*; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D009154:Mutation; D009281:Naphthalenes / Q000494:pharmacology*
D009281:Naphthalenes; C012258:mevastatin; D008148:Lovastatin; D006903:Hydroxymethylglutaryl CoA Reductases