Interactions of C12 surfactants with the skin: studies on enzyme release and percutaneous absorption in vitro.
Using an in vitro penetration cell, it has been shown that enzymes (acid phosphatase, lactate dehydrogenase and N-acetylglucosaminidase) are released from rat-skin slices in response to contact with two irritant C12 surfactants, sodium laurate and sodium lauryl sulphate, but not with the non-irritant sodium lauroyl isethionate. About 3-5 hr contact of the stratum corneum with surfactant and a long incubation time (24 hr) were required for enzyme release. Adsorption and penetration of the two effective surfactants was also studied and the results for sodium lauryl sulphate suggested a relationship between enzyme release and adsorption of surfactant. However, no such simple relationship was observed for sodium laurate, emphasizing the complex nature of surfactant interactions with the skin.
D000327:Adsorption; D000818:Animals; D066298:In Vitro Techniques; D007509:Irritants; D007850:Lauric Acids / Q000378:metabolism / Q000633:toxicity; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000187:drug effects* / Q000201:enzymology; D012869:Skin Absorption; D012967:Sodium Dodecyl Sulfate / Q000378:metabolism / Q000633:toxicity; D013501:Surface-Active Agents / Q000378:metabolism / Q000633:toxicity*
D007509:Irritants; D007850:Lauric Acids; D013501:Surface-Active Agents; C030358:lauric acid; D012967:Sodium Dodecyl Sulfate