Selective isolation of tryptophan-containing peptides by hydrophobicity modulation.
Tryptophan-containing peptides are selectively isolated from complex digests by taking advantage of changes in hydrophobicity and chromatographic mobility induced by reaction with o-nitrophenylsulfenyl chloride. The peptides are first located in crude fractions by monitoring the fluorescence during high-performance liquid chromatography and then chemically modified to facilitate their separation from contaminants during subsequent rechromatography.
D000596:Amino Acids / Q000302:isolation & purification; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D010455:Peptides / Q000302:isolation & purification*; D012995:Solubility; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000302:isolation & purification*
D000596:Amino Acids; D010455:Peptides; D014364:Tryptophan