title: Promotion of epithelial cell adhesion on collagen by proteins from rat embryo fibroblasts.
abstract: We have found that the promoting effects of proteins from fibroblast conditioned medium (PCM) promote adhesion of rat liver epithelial cells (nonmalignant RL-34 cell line) seeded on collagen coated substratum. Since the effect was completely inhibited by treating the cells with cycloheximide the protein(s) are probably produced by the RL-34 cells. We also found that PCM-A fraction which is identical to fibronectin purified from whole PCM had no effect on RL-34 cell adhesion and that the adhesion was not inhibited even by adding affinity purified anti-FN antibody to the medium containing FN-free PCM (PCM-B fraction). This indicates that adhesion of rat liver epithelial cells on collagen substratum does not require FN, but requires the adhesion promoting factor(s) existing in PCM-B.
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D003094:Collagen; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D004622:Embryo, Mammalian; D004847:Epithelial Cells; D005347:Fibroblasts; D005353:Fibronectins / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology*; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D003470:Culture Media; D005353:Fibronectins; D011506:Proteins; D003094:Collagen; D003513:Cycloheximide