title: Analysis of compaction in the preimplantation mouse embryo.
abstract: An SEM analysis of the effects of tunicamycin, cytochalasin B, and colcemid has yielded insights into the process of compaction in the early mouse embryo. All three reagents block or reverse compaction and decrease the number of microvilli (MV), although some MV polarization is permitted. In addition, tunicamycin is shown to lessen cell adhesion even in compacted embryos. Cytochalasin B causes the formation of MV clumps some of which are preferentially localized to the apex or lateral ring region. Colcemid reverses compaction and, coupled with Pronase treatment, completely blocks compaction of uncompacted 8-cell embryos. Observations also suggest that MV polarization can occur only once but compaction (the close adherance and flattening of blastomeres) can be reversed and reinduced. Evidence is consistent with a three-step compaction process involving (1) cell surface recognition and attachment of a ring of lateral microvilli to adjacent blastomeres, (2) subsequent microfilament shortening in these lateral MV, and (3) maintenance of the compacted and polarized state by microtubules.
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D003572:Cytochalasins / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology; D003703:Demecolcine / Q000494:pharmacology; D047108:Embryonic Development; D005260:Female; D051379:Mice / Q000196:embryology*; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000187:drug effects; D011247:Pregnancy; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D003572:Cytochalasins; D014415:Tunicamycin; D003703:Demecolcine