[Separation of cholesterol-specific cytochrome P-450 regions and their localization in the polypeptide chain].
The separation of the two domains, disclosed by limited trypsinolysis in the cholesterol side chain cleavage cytochrome P-450, by covalent chromatography on thiopropyl-Sepharose 6B is described. The domains F1 (MW 27 000) and F2 (MW 22 000) are shown to belong to the N-terminal and C-terminal regions of the polypeptide chain respectively.
D000302:Adrenal Cortex / Q000201:enzymology; D000595:Amino Acid Sequence; D000818:Animals; D002784:Cholesterol; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification*; D006868:Hydrolysis; D008928:Mitochondria / Q000201:enzymology; D010446:Peptide Fragments; D010455:Peptides; D014357:Trypsin
D010446:Peptide Fragments; D010455:Peptides; D003577:Cytochrome P-450 Enzyme System; D002784:Cholesterol; D014357:Trypsin
Razdelenie domenov kholesterinspetsificheskogo tsitokhroma P-450 i ikh lokalizatsiia v polipeptidnoĭ tsepi.