Exodeoxyribonuclease from rat brain specific for single-stranded DNA.
An exodeoxynuclease with single-stranded DNA specificity has been isolated from rat brain and purified to electrophoretic homogeneity. Approximately 1100-fold purification with a yield of 16% has been achieved by chromatography on DNA-agarose, hydroxyapatite and Sephadex G-200. The enzyme has a pH optimum at 8.4, requires Mg2+ or Mn2+ as a cofactor and has a molecular weight of about 60000. It hydrolyzes homologous, heterologous, synthetic and depurinated substrates at the same rate liberating nucleoside 5'-monophosphates but does not attack ultraviolet-irradiated polydeoxyribonucleotides. This DNase is localized predominantly in neuronal cell nuclei and appears to be lacking in rat liver tissue.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002467:Cell Nucleus / Q000201:enzymology; D055598:Chemical Phenomena; D002621:Chemistry; D004277:DNA, Single-Stranded / Q000378:metabolism*; D005090:Exodeoxyribonucleases / Q000302:isolation & purification*; D006868:Hydrolysis; D009474:Neurons / Q000201:enzymology; D051381:Rats; D012995:Solubility; D013379:Substrate Specificity
D004277:DNA, Single-Stranded; D005090:Exodeoxyribonucleases; C040254:DNase brain III