Light and ultrastructural characteristics of neuroblastoma glioma hybrid NG 108-15 cells.
"Undifferentiated" neuroblastoma glioma hybrid NG 108-15 cells have not previously been characterized at a light and ultrastructural level despite their use in opiate receptor studies and their possession of one or more opioids. Three cell types rather than one were found. Type A is the classic round hybridoma cell. Type B is a neuron like cell and Type C is a giant cell similar to Type A cells but larger and with dense cytoplasm. Dense core vesicles, 800-1200 Ao in diameter were found as were virus particles in most Type A cells. Multivesicular bodies with clear vesicles which are interpreted as glutaraldehyde artefacts were found but no clear neurosecretory vesicles were found in any A cells--Etorphine at 10(-6) M for 48 hours and naloxone 10(-4) M were employed to product dependence and precipitated abstinence but no clear cut morphologic differences were noted between control, opiate dependent and precipitated abstinent cells.
D000818:Animals; D002460:Cell Line; D005048:Etorphine / Q000494:pharmacology; D005910:Glioma / Q000473:pathology*; D006822:Hybrid Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D051379:Mice; D009270:Naloxone / Q000494:pharmacology; D009447:Neuroblastoma / Q000473:pathology* / Q000648:ultrastructure; D051381:Rats
D009270:Naloxone; D005048:Etorphine