Detection of a feline X-linked antigen in somatic cell hybrids. Single-cell analysis using monoclonal antibodies.
We tested the premise that monoclonal antibodies to either intracellular or membrane antigens can greatly facilitate the construction of linkage maps of mammals whose chromosomes can be introduced into rodent cells. Monoclonal antibodies against antigenic determinants of cat lymphocytes and fibroblasts were used to analyze feline antigen expression in cat-mouse somatic cell hybrid populations selected to contain the X-linked feline HPRT locus. The frequency of antigen expression as measured by fixed cell immunofluorescence (IF) assays, varied greatly within hybrid populations for all but the antigen designated as VP382. Its frequent presence in hybrid cells led to the prediction, confirmed by 8-azaguanine selection experiments, that its expression was controlled by a gene, or genes, on the feline X chromosome. The antigens identified by the rest of the antibodies segregated independently of each other in cat-mouse somatic cell hybrids and their expression appeared to be controlled by autosomal genes of the cat.
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000235:genetics*; D002415:Cats; D004798:Enzymes / Q000235:genetics; D005260:Female; D008040:Genetic Linkage; D006822:Hybrid Cells / Q000201:enzymology / Q000276:immunology*; D051379:Mice; D012730:Sex Chromosomes; D014960:X Chromosome
D000911:Antibodies, Monoclonal; D000941:Antigens; D004798:Enzymes