A facile method for the isolation of porcine heart mitochondrial malate dehydrogenase by affinity elution chromatography on Procion Red HE3B.
A quick, simple method has been devised for isolating pig heart mitochondrial malate dehydrogenase in apparently homogeneous state and good yield. It entails the adsorption of the enzyme to agarose-linked Procion Red HE3B and specific elution of a ternary complex consisting of the malate dehydrogenase, NAD+, and L-malate.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D004396:Coloring Agents; D008291:Malate Dehydrogenase / Q000302:isolation & purification*; D008293:Malates; D008929:Mitochondria, Heart / Q000201:enzymology*; D009243:NAD; D013552:Swine; D014227:Triazines
D004396:Coloring Agents; D008293:Malates; D014227:Triazines; D009243:NAD; C020795:procion red HE-3B; C030298:malic acid; D008291:Malate Dehydrogenase