Current high-performance liquid chromatographic methodology in analysis of nucleotides, nucleosides, and their bases. I.
This review of HPLC technology is presented in two parts. An introduction to chromatography, sample preparation, and basic information on chromatographic theory and terminology, modes, and instrumentation is presented here. A discussion of stationary and mobile phase parameters which can be varied to optimize separations is also presented here. The second part of the review, which will appear in the next issue, will cover peak identification, quantification, and selected applications. The applications include short discussions of applications of HPLC for metabolic profiling, for assays of adenosine and cyclic nucleotides in studies of disease processes, and for enzyme assays. The paper concludes with a short discussion of future trends for HPLC in biomedical research and clinical chemistry.
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009705:Nucleosides / Q000032:analysis*; D009711:Nucleotides / Q000032:analysis*; D009994:Osmolar Concentration; D012997:Solvents
D009705:Nucleosides; D009711:Nucleotides; D012997:Solvents