Use of non-physiological buffer systems in the analysis of methotrexate transport in L1210 cells.
Methotrexate transport parameters have been compared in L1210 cells suspended in a series of HEPES buffer systems of varying ionic compositions. While no effect was observed on the Vmax for methotrexate influx, the Kt for half-maximal influx, the steady-state level of methotrexate, and the efflux rate each varied substantially and to an extent which could be correlated directly to the anionic composition of the external medium. Buffer composition also affected the membrane potential, the ATP level of the cells, and, in one instance, the cell volume, but these changes did not exert a significant effect on the transport process. These results suggest that the integrity of L1210 cells is not adversely affected by either the presence of HEPES in the suspending medium or by the absence of certain physiological ions, and, moreover, that methotrexate transport parameters measured under these conditions, although not necessarily indicative of the quantitative events that might occur in vivo, can nevertheless provide meaningful information on the properties and mechanism of this transport system.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002021:Buffers; D007939:Leukemia L1210 / Q000378:metabolism* / Q000473:pathology; D008564:Membrane Potentials; D008727:Methotrexate / Q000378:metabolism*; D051379:Mice; D009994:Osmolar Concentration
D002021:Buffers; D000255:Adenosine Triphosphate; D008727:Methotrexate