Interferon effects on multiplication, cytoplasmic protein and GFAP content, and morphology in human glioma cells.
Beta-type human fibroblast interferon inhibited the multiplication of human glioma cells in a dose-dependent manner. The inhibitory effects were thought to be due to suppression of the cells entering the S-phase. After interferon treatment with 5 X 10(3) IU/ml for ten days, the mean cell volume, the soluble protein, and the glial fibrillary acidic protein increased to 970%, 190%, and 380% that of the controls, respectively. The interferon-treated cells changed shape and resembled mature astrocytes. It is presumed that beta-type human fibroblast interferon inhibits DNA synthesis of cultured glial cells and subsequently induces specific differentiated proteins and morphologic alteration.
D002455:Cell Division; D002460:Cell Line; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005904:Glial Fibrillary Acidic Protein; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism
D004273:DNA, Neoplasm; D005904:Glial Fibrillary Acidic Protein; D007370:Interferon Type I; D007381:Intermediate Filament Proteins; D009363:Neoplasm Proteins; D013936:Thymidine