Effect of canavanine and 2,3-dimercapto-1-propanol (British antilewisite) on the proliferation of Novikoff hepatoma cells in the presence of concanavalin A.
The effect of canavanine and 2,3-dimercapto-1-propanol (British antilewisite, BAL) on the uptake of (3H)-thymidine by Novikoff hepatoma cells, as a measure of DNA formation, was measured in tissue culture in the absence and presence of the mitogen, concanavalin A (Con A). Canavanine and Con A stimulated cell proliferation at low concentrations, and inhibited at higher concentrations. BAL inhibited even at low concentrations. In the presence of a stimulating concentration of Con A, both canavanine and BAL inhibitory effects were amplified. In the presence of a high concentration of Con A and low concentration of BAL the combined effect was substantially greater than the sum of the individual effects. We describe the culture and harvest of Novikoff hepatoma cells, and changes in their morphology when acted upon by the toxic agents used.
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols; D002172:Canavanine / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D004112:Dimercaprol / Q000494:pharmacology*; D005260:Female; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D013936:Thymidine / Q000378:metabolism
D004112:Dimercaprol; D003208:Concanavalin A; D002172:Canavanine; D013936:Thymidine