Morphometric synaptology of a whole neuron profile using a semiautomatic interactive computer system.
We propose a new method of dealing with morphometric synaptology that processes all synapses and boutons around the HRP marked neuron on a large composite electron micrograph, rather than a qualitative or a piecemeal quantitative study of a particular synapse and/or bouton that is not positioned on the surface of the neuron. This approach requires the development of both neuroanatomical procedures, by which a specific whole neuronal profile is identified, and valuable specialized tools, which support the collection and analysis of a great volume of morphometric data from composite electron micrographs, in order to reduce the burden of the morphologist. The present report is also concerned with the total and reliable semi-automatic interactive computer system for gathering and analyzing morphometric data that has been under development in our laboratory. A morphologist performs the pattern recognition portion by using a large-sized tablet digitizer and a menu-sheet command, and the system registers the various morphometric values of many different neurons and performs statistical analysis. Some examples of morphometric measurements and analysis show the usefulness and efficiency of the proposed system and method.
D000818:Animals; D002415:Cats; D003201:Computers; D003712:Dendrites / Q000648:ultrastructure; D006735:Horseradish Peroxidase; D008854:Microscopy, Electron; D009474:Neurons / Q000648:ultrastructure*; D012984:Software; D013569:Synapses / Q000648:ultrastructure*; D013572:Synaptic Vesicles / Q000648:ultrastructure
D006735:Horseradish Peroxidase