[Physiological dependency between the hypothalamus and the thymus in Wistar rats. III. Hypothalamic cells cultured in vitro in the presence of Thymus Factor X (TFX) and fresh thymus filtered homogenate].
The studies were carried out on the hypothalamic cells cultured in vitro in the presence of low-molecular proteins (constituting the control): the thymic hormone, known as Thymus Factor X [TFX]; or fresh thymus filtered homogenate prepared from the glands collected from 1 h or 7 d old rats. All the cultures were supplemented with 3H-thymidine. The experiments demonstrated that TFX in when added to the culture caused a decrease 3H-thymidine synthesis in comparison to the control. In other cultures, which were enriched with the fresh thymus filtrated homogenate, especially from 7 d old rats, a high level of thymidine synthesis in the cellular nuclei was observed.
D000818:Animals; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000648:ultrastructure; D046508:Culture Techniques; D007031:Hypothalamus / Q000166:cytology*; D008297:Male; D008871:Microvilli / Q000648:ultrastructure; D008938:Mitosis; D009474:Neurons / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D013935:Thymic Factor, Circulating / Q000502:physiology*; D013949:Thymus Extracts / Q000502:physiology*; D013950:Thymus Gland / Q000166:cytology*; D013951:Thymus Hormones / Q000502:physiology*
C019108:TFX; D013949:Thymus Extracts; D013951:Thymus Hormones; D013935:Thymic Factor, Circulating