[Enzymatic synthesis of oligonucleotides corresponding to the 3'-terminus of influenza virus RNA].
Dodecanucleotide CpCpUpGpCpUpUpUpUpGpCpU corresponding to 3'-terminus of influenza virus RNA has been synthesized. The initial blocks CpCpU, GpCpU, pGpCpU and pGpCpUp were prepared enzymatically in the presence of ribonucleases of different substrate specificity and polynucleotide phosphorylase M. luteus. pUpUpU was obtained by enzymatic degradation of polyuridilic acid. The initial blocks were joined by T4 RNA-ligase.
D001483:Base Sequence; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D009841:Oligonucleotides / Q000096:biosynthesis*; D009975:Orthomyxoviridae / Q000378:metabolism*; D011118:Polynucleotide Ligases / Q000378:metabolism*; D011130:RNA Ligase (ATP) / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics
D009841:Oligonucleotides; D012367:RNA, Viral; D011118:Polynucleotide Ligases; D011130:RNA Ligase (ATP)
Fermentativnyĭ sintez oligonukleotidov, sootvetstvuiushchikh 3'-kontsu RNK virusa grippa.