Effects of NaCl on the uptake of alpha-[14C]aminoisobutyric acid by the halotolerant bacterium Halomonas elongata.
The alpha-aminoisobutyric acid (AIB) transport system of the halotolerant bacterium, Halomonas elongata, was examined. Cells were grown in L-alanine defined medium with 0.05, 0.375, 1.37, 2.5, or 3.4 M NaCl. Each group of cells was resuspended in buffered salts with different NaCl concentrations (0.05, 0.375, 1.37, 2.5, and 3.4 M) and the uptake of alpha-[14C]AIB was measured. Optimum AIB uptake occurred in the 0.375 M NaCl solution for the lower salt grown cells and the 1.37 M NaCl solution for the higher salt grown cells. When cells were grown in the higher salt media and suspended in hypoosmotic solutions, appreciable AIB uptake occurred; but for cells grown in lower salt media and suspended in hyperosmotic solutions, the uptake was dramatically reduced. This effect was mainly attributed to cell plasmolysis which in turn resulted in some cell death. The AIB uptake was Na+ specific and this analogue was not metabolized after being transported into the cells. An amino acid competition study gave a pattern similar to that of a marine bacterium.
D000596:Amino Acids / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D006216:Halobacteriaceae / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*
D000596:Amino Acids; D000621:Aminoisobutyric Acids; D012965:Sodium Chloride