[Degree of cell spreading on a substrate as the factor determining the nature of cell microrelief in suspension].
Quantitative ratio of various types of cell surface microrelief was determined in suspensions prepared from mouse monolayer cultures of embryo fibroblasts grown on different solid substrates: with high (Falcon) or low poly(2-hydroxyethylmethacrylate) adhesiveness; with flat or cylindrical (53-mu curvature radius) surfaces (polyvinylchloride). The electron microscopy revealed that poorly spread cells (on low adhesive or cylindrical substrata) in suspensions had the microvillous surface relief much more often than the cells brought to suspension from highly adhesive or flat substrata. Thus, the lower the degree of cell spreading on the substratum, the higher the probability for the cell to acquire the microvillous relief in suspended state. The microvillous relief of transformed cells in suspensions is, probably, due to their poor spreading on substrata in the monolayer cultures.
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured / Q000648:ultrastructure*; D003470:Culture Media / Q000494:pharmacology; D005347:Fibroblasts / Q000648:ultrastructure; D051379:Mice; D008809:Mice, Inbred C3H; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties; D013535:Suspensions
D003470:Culture Media; D013535:Suspensions
Stepen' rasplastannosti kletok na substrate kak faktor, opredeliaiushchiĭ kharakter ikh mikrorel'efa v suspenzii.