Long-term culture of human oligodendrocytes. Isolation, growth and identification.
Oligodendrocytes were isolated from adult human brains obtained at autopsy. The cells were prepared by Percoll density gradient centrifugation, seeded on plastic coverslips and were cultured for a period up to 6 months. The oligodendrocytes in culture expressed cell-type specific markers, galactocerebroside and myelin basic protein and revealed the ultrastructure of mature oligodendrocytes.
D000328:Adult; D000368:Aged; D001921:Brain / Q000473:pathology / Q000502:physiology*; D002455:Cell Division; D002469:Cell Separation; D002478:Cells, Cultured; D006801:Humans; D008854:Microscopy, Electron; D008875:Middle Aged; D009457:Neuroglia / Q000502:physiology*; D009836:Oligodendroglia / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure