Human X (mouse X human) hybridomas stably producing human antibodies.
A method is described for constructing mouse heterohybridomas producing human monoclonal antibody with a high stability. The essence of the method is the use of a nonsecretor mouse X human hybridoma which has been made 8-azaguanine resistant as a fusion parent for construction of (mouse X human) X human hybridomas. The production of four human anti-influenza antibodies is described. The cells have now been maintained for more than 22 months in culture.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000914:Antibodies, Viral / Q000096:biosynthesis; D001375:Azaguanine / Q000494:pharmacology; D002459:Cell Fusion; D004351:Drug Resistance; D006801:Humans; D006825:Hybridomas / Q000187:drug effects / Q000276:immunology*; D009980:Influenza A virus / Q000276:immunology; D051379:Mice
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D001375:Azaguanine