Production of a high affinity antibody specific to the calcium-free-form of calmodulin, using N-acetyl-muramyl-L-alanyl-D-isoglutamine-calmodulin conjugate.
Covalent coupling of N-acetyl-muramyl-L-alanyl-D--isoglutamine (MDP) to calmodulin was used in order to enhance its immunogenicity. Rabbit antibodies against calmodulin were obtained, interacting with the calcium--free-form of calmodulin. The radioimmunoassay was developed using the whole sera (titre 1:20,000) having a sensitivity of 20 pg per tube (50% displacement 500 pg/assay tube). The radioimmunoassay shows the same concentrations of calmodulin in rat brain homogenate (4.9 +/- 0.86 micrograms/mg protein), bovine neurosecretosomes (0.77 +/- 0.10 microgram/mg protein), and bovine neurohypophysial secretory vesicles (0.05 +/- 0.01 microgram/mg protein) as the phosphodiesterase activation method.
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000276:immunology*; D000818:Animals; D000917:Antibody Formation; D001923:Brain Chemistry; D002147:Calmodulin / Q000032:analysis / Q000276:immunology*; D002417:Cattle; D010904:Pituitary Gland, Posterior / Q000032:analysis; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D051381:Rats
D002147:Calmodulin; D000119:Acetylmuramyl-Alanyl-Isoglutamine