HPLC-RIA of a metabolite of norethindrone in human plasma, 17 alpha-ethinyl-5 beta-estrane-3 alpha, 17 beta-diol, following conjugate hydrolysis.
An HPLC-RIA method for the assay of a reduced metabolite of norethindrone, 17 alpha-ethinyl-5 beta-estrane-3 alpha, 17 beta-diol, is described. Conjugated metabolites were extracted from human plasma and subjected to hydrolysis prior to analysis. With a daily dose of 2 mg of norethindrone, the mean plasma level of the metabolite was 24.8 ng/ml with a range of 22.8 ng/ml to 33.9 ng/ml.
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006868:Hydrolysis; D009640:Norethindrone / Q000378:metabolism*; D009641:Norethynodrel / Q000031:analogs & derivatives* / Q000097:blood; D011863:Radioimmunoassay
C003578:17-ethynyl-5-estrane-3,17-diol; D009641:Norethynodrel; D009640:Norethindrone