title: Action of hydrolyzed cisplatin and some analogs on microtubule protein polymerization in vitro.
abstract: The inhibition of tubulin microtubule polymerization by cisplatin was investigated. The interaction was monitored by turbidity measurements and electron microscopy. For a 2.5 x 10(-5) M concentration of cisplatin, the inhibition at 37 degrees C was nearly 50% after 1 hour of incubation and 80% after 2 hours. A similar but lesser effect was observed after incubation at 4 degrees C. One analog of cisplatin, sulfato-aqua(1,2)diamminocyclohexane platinum II, showed the same inhibition effect.
mesh_terms: D002945:Cisplatin / Q000494:pharmacology*; D003078:Colchicine / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D008870:Microtubules / Q000648:ultrastructure; D011108:Polymers; D013329:Structure-Activity Relationship; D013696:Temperature; D014404:Tubulin / Q000378:metabolism*
chemical_list: D011108:Polymers; D014404:Tubulin; D002945:Cisplatin; D003078:Colchicine