In vitro incubation of lenses: a model for investigations on lens transparency by varying the perfusion rate, osmolarity and temperature of the culture medium.
A Beckman photometer was modified so that the unfiltered beam of a tungsten lamp could pass vertically through a special chamber. This particular chamber, containing two compartments, allowed certain modifications of the incubation conditions with respect to the anterior and/or posterior lens surface. The temperature of the incubation media was variable between 15 degrees and 37 degrees C; they were led into the chamber by hose gauge at different perfusion rates. The light transmission measured by the photometer was registered by a Honeywell recorder. Preliminary results of the various incubation conditions on the lens transparency of pig lenses are presented.
D000818:Animals; D002386:Cataract / Q000209:etiology*; D004195:Disease Models, Animal; D009924:Organ Culture Techniques; D009994:Osmolar Concentration; D010477:Perfusion; D011183:Postoperative Complications / Q000209:etiology; D013552:Swine; D013696:Temperature; D014821:Vitrectomy