The effect of ascorbic acid on the conjugation of 4-hydroxybiphenyl in rat isolated hepatocytes.
Ascorbic acid at high non-physiological levels inhibited the sulphation of 4-hydroxybiphenyl by rat isolated hepatocytes. Glucuronylation of 4-hydroxybiphenyl, formed from 4-methoxybiphenyl, was increased at ascorbic acid levels which inhibited sulphation. The glucuronylation of 4-hydroxybiphenyl added directly to the cells was enhanced at concentrations of ascorbic acid which did not inhibit sulphation. Ascorbic acid did not influence the cytochrome P-450-dependent O-demethylation of 4-methoxybiphenyl or the level of cellular lipid peroxidation.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism*; D005965:Glucuronates / Q000378:metabolism; D066298:In Vitro Techniques; D008658:Inactivation, Metabolic / Q000187:drug effects; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013431:Sulfates / Q000378:metabolism
D001713:Biphenyl Compounds; D005965:Glucuronates; D013431:Sulfates; C019046:4-phenylphenol; D001205:Ascorbic Acid