title: Incorporation of acylated wheat germ agglutinin into liposomes.
abstract: Purified wheat germ agglutinin (WGA) was derivatized with palmitic acid at an average stoichiometry of one fatty acid per dinner. Palmitoyl WGA was readily incorporated into liposomes with a cholate-dialysis method. Liposome-bound WGA caused agglutination of red blood cells at a concentration eight-fold lower than that of the native lectin. Furthermore, enhanced binding of liposome-bound WGA to mouse spleen cells was also observed. Potential applications of the liposome-bound lectin are discussed.
mesh_terms: D000818:Animals; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D006386:Hemagglutination Tests; D066298:In Vitro Techniques; D037102:Lectins / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D013154:Spleen / Q000378:metabolism; D014909:Wheat Germ Agglutinins
chemical_list: D037102:Lectins; D008081:Liposomes; D014909:Wheat Germ Agglutinins