Biological evaluation of Wratizolin penetration from ointments to the skin.
The degree of Wratizolin penetration to the skin from ointments prepared in 6 different media was determined. Experiments were carried out in mice and guinea pigs using: a) method determining the decrement of cytotoxic and antibacterial activity of the tested ointment contacted with the skin, b) biological tests to check the presence-of the drug in tissue extracts. Absorption of Wratizolin appeared dependent upon the kind of base ointment. The preparation penetrated well when suspended in polyethylene glycol base ointment (PEG).
D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology / Q000627:therapeutic use; D000998:Antiviral Agents / Q000378:metabolism* / Q000494:pharmacology / Q000627:therapeutic use; D001681:Biological Assay / Q000379:methods; D002478:Cells, Cultured; D002642:Chick Embryo; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D006168:Guinea Pigs; D006558:Herpes Genitalis / Q000188:drug therapy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009824:Ointments; D012869:Skin Absorption; D013211:Staphylococcus aureus / Q000187:drug effects; D013844:Thiazoles / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D000998:Antiviral Agents; D009824:Ointments; D013844:Thiazoles; C027999:5-(benzoylamino)-N-(4-chlorophenyl)-3-methyl-4-isothiazolecarboxamide