[Experiments with Bacillus thuringiensis protoplasts. I. Isolation of protoplasts and their reversion to bacillary form].
A method for protoplastization of crystal- and spore-forming Bacillus thuringiensis bacterian and consequent cell wall regeneration on a solid hypertonic medium is presented. Up to 50% of the protoplasts prepared were viable and formed colonies under special conditions; at the same time, less than 0,01% of the cells treated with lysozyme were resistant to the osmotic shock; bacterial autolytic system takes part in protoplasts formation. Electron microscopic studies of protoplasts and cells confirm the fact of cell wall removal and support the proposed mechanism of protoplast formation.
D001413:Bacillus thuringiensis / Q000187:drug effects / Q000235:genetics* / Q000648:ultrastructure; D002473:Cell Wall / Q000187:drug effects / Q000648:ultrastructure; D008854:Microscopy, Electron; D009113:Muramidase / Q000494:pharmacology; D011523:Protoplasts / Q000187:drug effects / Q000648:ultrastructure*
D009113:Muramidase
Eksperimenty s protoplastami Bacillus thuringiensis. Soobshchenie I. Poluchenie protoplastov i ikh reversiia v batsilliarnuiu formu.