Stimulation of cynomolgus peripheral blood lymphocytes with tetanus toxoid and smallpox vaccine.
The cynomolgus monkey was studied as an animal model to investigate the cell-mediated immunity induced by vaccines. Optimal conditions are described to isolate peripheral blood lymphocytes. Lymphocyte transformation tests were performed with tetanus toxoid and smallpox vaccine. Antigen-specific lymphocyte transformations with smallpox vaccine could only be demonstrated when lymphocytes were obtained from vaccinated monkeys. Tetanus toxoid appeared to be a weak antigen. However, after adsorption of the toxoid to aluminum phosphate, a significant antigen-specific lymphocyte transformation was observed.
D000327:Adsorption; D017607:Aluminum Compounds; D000818:Animals; D007111:Immunity, Cellular / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D008251:Macaca / Q000276:immunology*; D008252:Macaca fascicularis / Q000276:immunology*; D008992:Monkey Diseases / Q000276:immunology; D010710:Phosphates / Q000494:pharmacology; D012900:Smallpox Vaccine / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology*; D014611:Vaccination
D017607:Aluminum Compounds; D010710:Phosphates; D012900:Smallpox Vaccine; D013745:Tetanus Toxoid; C012714:aluminum phosphate