A study of intestinal copper-binding proteins in mottled mice.
The substantial retention of Cu2+ and to a lesser extent Zn2+, in the gut mucosa of neonatal MO mutant mice is largely associated with a low molecular weight protein tentatively identified as metallothionein. [35S]Cysteine incorporation into this protein in mutant mice is elevated, indicating that Cu2+ retention in the gut is associated with an increase in the synthesis of metallothionein. The high Cu2+ levels of mutant gut tissue decline rapidly with age to reach an approximately normal level by 24 days of age; this decline cannot be prevented by dietary supplementation and it is suggested that gut 'closure' and consequent reduced uptake by pinocytosis are important factors in this decline.
D000375:Aging; D000818:Animals; D001928:Brain Diseases, Metabolic / Q000378:metabolism*; D002352:Carrier Proteins / Q000302:isolation & purification; D003300:Copper / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D007706:Menkes Kinky Hair Syndrome / Q000378:metabolism*; D008667:Metalloproteins / Q000378:metabolism*; D008668:Metallothionein / Q000378:metabolism*; D051379:Mice; D008817:Mice, Mutant Strains; D008954:Models, Biological; D015032:Zinc / Q000378:metabolism
D002352:Carrier Proteins; D008667:Metalloproteins; C023548:copper-binding protein; D003300:Copper; D008668:Metallothionein; D015032:Zinc; D003545:Cysteine