GABA, hypotaurine and taurine transport in brain slices from developing mouse.
The kinetics and sodium-dependence of gamma-aminobutyric acid (GABA), hypotaurine and taurine uptake were analyzed in cerebral slices prepared from mice of different postnatal ages. The maximal velocities of transport (V) and the Km constants for the high-affinity uptake all decreased with age, whereas the developmental changes in the low-affinity uptake were exactly opposite. Both high- and low-affinity uptakes were sodium-dependent in all age groups. The general patterns of developmental changes were similar with all three amino acids, in keeping with the assumption of a common transport system for all of them.
D000367:Age Factors; D000818:Animals; D001921:Brain / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D051379:Mice; D012964:Sodium / Q000502:physiology; D013570:Synaptic Membranes / Q000378:metabolism; D013654:Taurine / Q000031:analogs & derivatives* / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D013654:Taurine; D005680:gamma-Aminobutyric Acid; C003949:hypotaurine; D012964:Sodium