A nonspecific inhibitor of hemagglutination by Tahyna virus in acetone-extracted mouse sera and its removal.
A nonspecific inhibitor of hemagglutination of Tahyna virus was present in acetone-extracted sera of 3-week old ICR mice but in a much lower level in the 3-day old mice of the same strain. This inhibitor did not neutralize Tahyna virus in vitro and did not protect mice against the lethal challenge by this virus. The inhibitor could not be removed by treatment with: acetone; kaolin, trypsin; heating at 56 degrees C for 30 min or 60 degrees C for 20 min, filtration through Seitz EK pads, a successive treatment with either acetone + trypsin, acetone + filtration, acetone + pH 2.0, or trypsin + heating. However, the inhibitory activity was eliminated by a combined treatment with acetone and heat (56 degrees C/30 min) - or vice versa, or by the isolation of gammaglobulin with the use of rivanol. Fractionated precipitation of serum proteins suggested that the nonspecific inhibitor is a lipoprotein, this being supported by a comparison of the lipoprotein content between samples with the inhibitor present and removed. The course of specific globulin (IgG and IgM) production in mice infected with Tahyna virus was studied in hemagglutination-inhibition test (HIT) using the sera treated with acetone plus heat. This combined treatment of sera might also be suggested for use in routine serological surveys (HIT) of small rodents with Tahyna virus antigen.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000998:Antiviral Agents / Q000276:immunology* / Q000302:isolation & purification; D002043:Bunyaviridae / Q000276:immunology*; D002141:Encephalitis Virus, California / Q000276:immunology*; D004670:Encephalitis, California / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male; D008623:Mercaptoethanol / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR
D000914:Antibodies, Viral; D000998:Antiviral Agents; D007075:Immunoglobulin M; D008623:Mercaptoethanol