title: Identification of two cDNA clones coding for androgen-dependent polypeptides in rat ventral prostate.
abstract: cDNA clones were obtained by transformation of E. coli chi 1776 with pBR322 containing insert of ds cDNA synthesized from total rat prostate poly(A)RNA. Two prostate-specific cDNA clones were isolated by colony hybridization and identified by message selection/translation as encoding polypeptides of Mr: 13,500 and 9,300. Hybridization of poly(A)RNA from normal and castrated rat prostates to the cloned cDNAs indicated that the levels of mRNAs coding for Mr: 13,500 and 9,300 polypeptides are regulated by testosterone.
mesh_terms: D000728:Androgens / Q000378:metabolism*; D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D008297:Male; D009693:Nucleic Acid Hybridization; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics*; D011467:Prostate / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000728:Androgens; D010455:Peptides; D004247:DNA