Phagocytosis of senescent erythrocytes by autologous monocytes: requirement of membrane-specific autologous IgG for immune elimination of aging red blood cells.
The role of membrane-bound IgG present on the membrane of senescent erythrocytes in immune eliminations of aging red cells was investigated. Phagocytosis of populations of red blood cells (RBC) of different ages by autologous monocytes was assessed both by direct phagocytosis and by induction of microsomal heme oxygenase. Removal of IgG from older RBCs inhibited their phagocytosis; in contrast, preincubation of neuraminidase-treated young or in vitro aged RBCs with IgG eluted from old cells led to phagocytosis of RBCs treated by autologous monocytes. It was also found that the Fc portion of membrane-bound IgG is essential for the elimination of senescent cells; less than 15% of old heat-inactivated RBCs coated with F(ab)2 fragment of membrane-bound IgG were phagocytosed. In contrast, more than 50% of old heat-inactivated RBCs coated with heat-eluted IgG were phagocytosed by autologous monocytes. A possible mechanism of elimination of aged cells is discussed.
D000328:Adult; D001666:Binding Sites, Antibody; D004905:Erythrocyte Aging; D004910:Erythrocyte Membrane / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006419:Heme Oxygenase (Decyclizing) / Q000097:blood; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000378:metabolism*; D008297:Male; D009000:Monocytes / Q000276:immunology*
D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D006419:Heme Oxygenase (Decyclizing)