Peptide studies using a fast atom bombardment high field mass spectrometer and data system. 1--Sample introduction, data acquisition and mass calibration.
Conditions were established for analyzing as little as 5 pmol of an underivatized peptide delivered in a glycerol sample matrix as a thin film onto a gold-plated copper sample stage and then bombarded with xenon fast atoms. Calibration of the fast atom bombardment high field mass spectrometer and data system was achieved using cesium iodide/glycerol as a reference. Calibration at several accelerating potentials permitted a mass range from 393 to 5941 u. Several factors were examined that contribute to the quality of the mass spectrum: components within the glycerol such as other peptides, alkali salts, acid and reducing agents; the nature of the fast atom gas; concentration of the peptide delivered to the sample stage; and the effect of the sample stage and sample matrix on sensitivity.
D000803:Angiotensin I / Q000032:analysis; D005755:Gastrins / Q000032:analysis; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008832:Microchemistry; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*; D013935:Thymic Factor, Circulating / Q000032:analysis
D005755:Gastrins; D010455:Peptides; D000803:Angiotensin I; D013935:Thymic Factor, Circulating