[Morphological aspect and chemical composition of Skeletonema costatum (Bacillariophyceae) growing in natural nutritive media with the aid of a culture system of dialysing fibers].
The growth of Skeletonema costatum, under natural nutriment conditions, was studied using a bulk culture fiber dialyzing apparatus. The diatom displayed normal development of chain length (average cell number per chain) which coincided with the culture growth stages; that is, the cell number per colony increased during the active division period and decreased thereafter with the beginning of the prestationary phase. This morphological behaviour showed that the alga cells were not affected by such physical shocks as collision or tension occurring during repeated cell transfers from growth chambers to the dialyzing apparatus or at the time of their passage through the fiber fascicles. Measured at different growth stages, the cell contents in carbon, nitrogen, and chlorophyll confirmed the above results and showed for S. costatum a biological productivity comparable with that obtained in smaller dialyzing containers (dialyzing bags). Through a comparison between the dialyzing culture and a static culture grown in an enriched medium, certain characteristics were determined.
D003470:Culture Media; D003956:Dialysis; D056890:Eukaryota / Q000032:analysis / Q000166:cytology / Q000254:growth & development*
D003470:Culture Media
Aspect morphologique et composition chimique de Skeletonema costatum (Bacillariophyceae) croissant en milieu nutritif naturel à l'aide d'un système de culture à fibres dialysantes.