Isolation of HuIFN-beta by immunosorbent and high-pressure liquid chromatography.
A procedure for isolating HuIFN-beta without the use of SDS or other denaturants has been developed. The procedure involves the chromatography of IFN on an antibody affinity column followed by reversed-phase HPLC. The specific activity of the resulting IFN-beta is 4 X 10(8) u/mg protein, and overall recovery is about 76%. The procedure provides a method for isolating pure HuIFN-beta in its native form with an efficiency much higher than any other method reported to date.
D002478:Cells, Cultured; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D005347:Fibroblasts / Q000032:analysis; D006801:Humans; D007370:Interferon Type I / Q000276:immunology / Q000302:isolation & purification*; D008297:Male
D007370:Interferon Type I