Influence of chronic ethanol consumption on muscarinic cholinergic receptors and their linkage to phospholipid metabolism in mouse synaptosomes.
C57/BL mice rendered physically dependent to ethanol exhibited an increase in specific binding of [3H]QNB to a cholinergically enriched synaptosomal fraction of brain without a parallel increase in muscarinic receptor-stimulated incorporation of [32P] into phosphatidylinositol and phosphatidic acid. Receptor-enhanced phospholipid labeling was reduced by the addition of ethanol (100mM) in vitro. In chronically treated mice, tolerance developed to the inhibitory effects of ethanol on receptor-enhanced phospholipid labeling. It is suggested that the inhibition of this response by ethanol added in vitro may be associated with alterations in neuronal function during alcohol intoxication.
D000437:Alcoholism / Q000503:physiopathology; D000818:Animals; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D010712:Phosphatidic Acids / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000187:drug effects*; D011976:Receptors, Muscarinic / Q000187:drug effects*; D013574:Synaptosomes / Q000187:drug effects*; D014316:Tritium
D010712:Phosphatidic Acids; D010716:Phosphatidylinositols; D010743:Phospholipids; D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D014316:Tritium; D000431:Ethanol; D011813:Quinuclidinyl Benzilate