[Interaction of DNA with N-bromosuccinimide. G+C-specific reaction potentially useful for structural and functional studies].
Incomplete modification of DNA fragments labeled at one terminus with N-bromosuccinimide is shown to be useful for localization of G and C residues along the polynucleotide chain. After N-bromosuccinimide treatment the fragments can be split at modified residues with piperidine. The rate of the reaction of the single-stranded fragments is extremely fast and much higher than that of double-stranded DNA. The modification can be used for structure and function studies on DNA.
D001483:Base Sequence; D001974:Bromosuccinimide; D003562:Cytidine; D004247:DNA; D006151:Guanosine; D013388:Succinimides
D013388:Succinimides; D006151:Guanosine; D003562:Cytidine; D004247:DNA; D001974:Bromosuccinimide
Vzaimodeĭstvie DNK s N-bromsuktsinimidom. G+C-spetsificheskaia reaktsiia, potentsial'no poleznaia dlia strukturnykh i funktsional'nykh issledovaniĭ.