Purification and separation of tomato isolectins by chromatofocusing.
Tomato lectin can be rapidly separated from a crude extract of tomato fruit proteins by chromatofocusing. The lectin is recovered from a PBE94 column in two peaks, each with a specific activity comparable to that of lectin purified by affinity chromatography on ovomucoid-Sepharose. Both isolectins consist of a single polypeptide chain (Mr 68,000) and have similar properties.
D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D037102:Lectins / Q000302:isolation & purification*; D010940:Plant Proteins / Q000302:isolation & purification*
D037102:Lectins; D010940:Plant Proteins