The high-performance liquid chromatographic analysis of diastereomers and structural analogs of angiotensins I and II.
Diastereomers, alpha- and beta-aspartic acid forms, and partial sequences of angiotensin I and II were resolved by reversed-phase high-performance liquid chromatography (HPLC). Nearly all of the peptides which were examined contained significant amounts of peptides whose amino acid composition differed from the designated peptide. This chromatographic procedure combined with amino acid analysis clearly offers the investigator a rapid method for analyzing and quantifying the purity of angiotensins which are intended for use as reference substances for radioimmunoassay and biological assay.
D000596:Amino Acids / Q000032:analysis; D000803:Angiotensin I / Q000031:analogs & derivatives / Q000032:analysis*; D000804:Angiotensin II / Q000031:analogs & derivatives* / Q000032:analysis*; D000809:Angiotensins / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D010446:Peptide Fragments / Q000032:analysis; D013237:Stereoisomerism
D000596:Amino Acids; D000809:Angiotensins; D010446:Peptide Fragments; D000804:Angiotensin II; D000803:Angiotensin I