Subunit structure of a potent platelet-activating glycoprotein isolated from the venom of Crotalus durissus cascavella.
The potent platelet-activating factor isolated from the venom of Crotalus durissus cascavella is an acid-soluble multisubunit glycoprotein of Mr 72,000 built up of two types of subunits, alpha and beta, linked by disulphide bonds. The mean apparent Mr of the reduced complex was around 12,000 by gel filtration under denaturating conditions. The Mrs of the alpha and beta subunits, with an apparent ratio of 1/1, were 12,600 and 13,580 by SDS-PAGE respectively. The Mr 72,000 glycoprotein is thought to be an alpha 3 beta 3 complex. The urea dissociated glycoprotein (Mr 72,000) retained its platelet-stimulating activity. It is concluded that the Mr 300,000 form isolated at acidic pH under native conditions, and showing a rosette - like, ring-shaped structure in the electron microscope as well as the Mr 144,000 form isolated at physiological pH under native conditions and active on platelets were the tetrameric and dimeric states of the molecule respectively.
D000818:Animals; D002850:Chromatography, Gel; D003435:Crotalid Venoms / Q000302:isolation & purification* / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D006023:Glycoproteins / Q000302:isolation & purification* / Q000494:pharmacology; D019791:Guanidine; D006146:Guanidines; D006863:Hydrogen-Ion Concentration; D037181:Lectins, C-Type; D046911:Macromolecular Substances; D008623:Mercaptoethanol / Q000494:pharmacology; D008854:Microscopy, Electron; D008970:Molecular Weight; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate; D014508:Urea
D003435:Crotalid Venoms; D006023:Glycoproteins; D006146:Guanidines; D037181:Lectins, C-Type; D046911:Macromolecular Substances; D012967:Sodium Dodecyl Sulfate; C028890:convulxin; D008623:Mercaptoethanol; D014508:Urea; D019791:Guanidine; D004229:Dithiothreitol