Complementation mapping in microcell hybrids: localization of Fpgs and Ak-1 on Mus musculus chromosome 2.
The gene encoding folylpolyglutamyl synthetase (FPGS) was assigned to mouse chromosome 2 by complementation mapping. Chinese hamster ovary cells (AuxBl) deficient in FPGS, and consequently auxotrophic for glycine, adenosine, and thymidine (gat-), were employed as recipients in microcell-mediated chromosome transfer experiments. Mouse chromosomes derived from diploid embryo fibroblasts were introduced into hamster AuxBl cells, and gat+ microcell hybrids were selected in medium lacking adenosine and thymidine. Mouse chromosome 2 was the only donor chromosome whose presence correlated with expression of FPGS activity. Furthermore, every gat+ hybrid clone expressed murine AK-1, a marker previously assigned to chromosome 2. Eight of 20 clones analyzed retained deletion chromosomes derived from mouse chromosome 2. These clones were used to localize murine Fpgs and Ak-1 to a region of this chromosome, namely 2 (cen leads to Cl).
D000241:Adenosine / Q000235:genetics; D000818:Animals; D002874:Chromosome Mapping; D006224:Cricetinae; D003412:Cricetulus; D005796:Genes; D005816:Genetic Complementation Test; D005998:Glycine / Q000235:genetics; D006822:Hybrid Cells / Q000201:enzymology*; D051379:Mice; D010453:Peptide Synthases / Q000235:genetics*; D013936:Thymidine / Q000235:genetics
D010453:Peptide Synthases; C023103:folylpolyglutamate synthetase; D000241:Adenosine; D005998:Glycine; D013936:Thymidine