Crosslinking of chromosomal proteins to DNA in HeLa cells by UV gamma radiation and some antitumor drugs.
Immunochemical techniques were used to investigate the protein-DNA crosslinking by ultraviolet (UV) and gamma radiation as well as 1,3-bis-(2-chloroethyl)-1-nitrosourea (BCNU) or cis- and trans-diamminedichloroplatinum II (cis-DDP and trans-DDP). Antisera to 0.35 M NaCl extract and 0.35 M NaCl residue of HeLa nuclei were employed. Both gamma and UV irradiation, exposure to cis- or trans-DDP and, to a lesser extent, BCNU, resulted in crosslinking of various antigens to the DNA. Although several antigens were crosslinked by all the employed agents, other exhibited agent-specific crosslinking patterns.
D002330:Carmustine / Q000494:pharmacology*; D002467:Cell Nucleus / Q000378:metabolism; D002945:Cisplatin / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000378:metabolism* / Q000528:radiation effects; D005720:Gamma Rays; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D007536:Isomerism; D009698:Nucleoproteins / Q000378:metabolism*; D011485:Protein Binding; D014466:Ultraviolet Rays
D004273:DNA, Neoplasm; D009698:Nucleoproteins; C034697:transplatin; D002945:Cisplatin; D002330:Carmustine