title: Dipetalonema viteae (Nematoda: Filarioidea): culture of third-stage larvae to young adults in vitro.
abstract: Infective third-stage larvae of Dipetalonema viteae (Nematoda: Filarioidea) were cultured to young adults in a cell-free culture system. Third-stage larvae from the tick vector grew, developed, and molted twice in a medium containing NCTC 135 and Iscove's modified Dulbecco's medium supplemented with fetal bovine serum under a gas phase of 95 percent nitrogen and 5 percent carbon dioxide. The availability of such a culture system for filariids should facilitate studies of their immunology, biochemistry, and sensitivity to drugs.
mesh_terms: D000818:Animals; D003470:Culture Media; D004153:Dipetalonema / Q000254:growth & development*; D066298:In Vitro Techniques; D007814:Larva; D008297:Male; D013987:Ticks / Q000469:parasitology
chemical_list: D003470:Culture Media