Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deduced from the amino acid sequence of bovine factor IX.
A unique 52mer oligonucleotide deduced from the amino acid sequence of bovine Factor IX was synthesized and used as a probe to screen a human liver cDNA bank. The Factor IX clone isolated shows 5 differences in nucleotide and deduced amino acid sequence as compared to a previously isolated clone. In addition, precisely one codon has been deleted.Images
D000409:Alanine; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D004247:DNA / Q000302:isolation & purification*; D005164:Factor IX / Q000235:genetics*; D006467:Hemophilia A / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000378:metabolism; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D013045:Species Specificity
D003062:Codon; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D005164:Factor IX; D004247:DNA; D000409:Alanine