Analysis of cytogenetic damage in rat lymphocytes following in vivo exposure to nitrobenzene.
Male F-344 rats were exposed to target concentrations of 0, 5, 16, or 50 ppm nitrobenzene for 6 h/day, 5 days a week, for 21 days during a 29-day period. Isolated spleen (ISLs) and peripheral blood lymphocytes (PBLs) were cultured in the presence of 2 microM 5-bromodeoxyuridine and scored for sister-chromatid exchange (SCE) and PBLs were also scored for chromosome aberrations. No significant increase in SCE frequency or chromosome aberrations was found in the PBLs, and no significant increase in SCEs was observed in the ISLs at any of the concentrations. Thus, cytogenetic analysis of ISLs and PBLs provide no evidence of a genotoxic potential for nitrobenzene.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002869:Chromosome Aberrations; D003520:Cyclophosphamide / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D009578:Nitrobenzenes / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D012854:Sister Chromatid Exchange / Q000187:drug effects
D009578:Nitrobenzenes; D003520:Cyclophosphamide; C036077:nitrobenzene; D001973:Bromodeoxyuridine