title: 3-aminopyridine 1,N6-ethenoadenine dinucleotide phosphate: a fluorescent reagent for NADP-requiring enzymes.
abstract: 3-Aminopyridine 1,N6-ethenoadenine dinucleotide phosphate (epsilon-AADP) was synthesized from 3-aminopyridine adenine dinucleotide phosphate by reaction with chloroacetaldehyde. The reaction was monitored by high-pressure liquid chromatography. The product, epsilon-AADP, was purified by ion-exchange chromatography followed by a Sephadex G-10 desalting process. Spectrophotometric and fluorimetric properties of epsilon-AADP were obtained at neutral pH. Diazotization of epsilon-AADP was documented by azodye formation at pH values below 3 and the diazotized derivative was shown to react rapidly with sulfhydryl compounds at neutrality. As an analog of NADP, epsilon-AADP was demonstrated to be an effective NADP-competitive inhibitor of NADP-requiring dehydrogenases, reductases, and an NAD glycohydrolase.
mesh_terms: D000227:Adenine Nucleotides / Q000138:chemical synthesis*; D002851:Chromatography, High Pressure Liquid; D004798:Enzymes / Q000032:analysis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D009249:NADP / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D000227:Adenine Nucleotides; D004798:Enzymes; D005456:Fluorescent Dyes; D009249:NADP; C039995:3-aminopyridine-1,N(6)-ethenoadenine dinucleotide phosphate