Effect of retinol and retinoic acid on P/O ratios of coupled mitochondria.
Retinol at levels above 0.5 n moles/mg protein and retinoic acid at levels above 0.25 n moles/mg protein are shown to uncouple mitochondrial oxidative phosphorylation. Uncoupling is measured with the substrates ascorbate plus N,N,N',N'-tetra-methyl-p-phenylenediamine, succinate and beta-hydroxybutyrate. These experiments indicate that the uncoupling effects of retinol and retinoic acid are not site specific but likely are the result of the vitamin being a general membrane bilayer disrupting agent.
D019297:2,4-Dinitrophenol; D020155:3-Hydroxybutyric Acid; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D004140:Dinitrophenols / Q000494:pharmacology; D006885:Hydroxybutyrates / Q000494:pharmacology; D066298:In Vitro Techniques; D008930:Mitochondria, Liver / Q000378:metabolism*; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects*; D051381:Rats; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid; D014212:Tretinoin / Q000494:pharmacology*; D014801:Vitamin A / Q000494:pharmacology*
D004140:Dinitrophenols; D006885:Hydroxybutyrates; D013386:Succinates; D014801:Vitamin A; D014212:Tretinoin; D019802:Succinic Acid; D001205:Ascorbic Acid; D019297:2,4-Dinitrophenol; D020155:3-Hydroxybutyric Acid