A simplified method to determine binding parameters in a two-site case using linear regression.
A program using a simplified method to compute estimates of binding parameters in a system with two independent classes of binding sites is presented. An iterative method is used to approximate step-by-step the two lines describing the binding activities separately, starting from a curvilinear Scatchard or Hofstee plot. This method is designed for use on microcomputers with graphic facilities and on programmable hand-held calculators.
D001665:Binding Sites; D003201:Computers; D006801:Humans; D008838:Microcomputers; D011869:Radioligand Assay; D012984:Software