title: Binding of plasma fibronectin to monolayer human endothelium.
abstract: Interaction of exogenous plasma fibronectin with endothelium was examined using monolayer cultures of human umbilical vein endothelial cells. Plasma fibronectin was purified on gelatin-sepharose with 1 M arginine elution and iodinated with 125I by the solid phase glycoluril method. Endothelial monolayers were incubated with 0.5 to 15.0 mg/l (125I)fibronectin. Specificity of binding was 50 to 60% as determined by competition with 50-fold excess nonlabeled fibronectin. Binding reached maximal levels within 1 hour and without saturation over the concentration range. The bound glycoprotein showed minimal dissociation during subsequent incubation in media free of fibronectin.
mesh_terms: D002478:Cells, Cultured; D004727:Endothelium / Q000378:metabolism*; D005353:Fibronectins / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D014471:Umbilical Veins
chemical_list: D005353:Fibronectins; D007457:Iodine Radioisotopes