Studies on the physical state of water in living cells and model systems. II. NMR relaxation times of water protons in aqueous solutions of gelatin and oxygen-containing polymers which reduce the solvency of water for NA+, sugars, and free amino acids.
This communication reports our study of the NMR relaxation times, T1 and T2 of water protons in aqueous solutions of bovine serum albumin, gelatin, polyvinylpyrrolidone, poly(ethylene oxide), and polyvinylmethylether over a wide concentration range. In contrast to solutions of gelatin and bovine serum albumin, the T1/T2 ratio of the three synthetic polymers are close to unity over the entire range studied. When combined with earlier-reported data of water made "non-solvent" to Na salts, the present data provided the basis for calculating the T1 and T2 as well as the rotational correlation time tau c of the "non-solvent" water. It was shown that only a modest increase by a factor of about 3 of tau c is enough to produce water that is "non-solvent" for Na citrate and sulfate. The new data reconciles NMR data of living cells with the theory of the cell water given in the association-induction hypothesis. The variability of tau c of "non-solvent" water also offers explanations of apparently conflicting conclusions on the physical state of cell water from dielectric measurements.
D000596:Amino Acids; D002241:Carbohydrates; D002414:Cations, Monovalent; D003593:Cytoplasm / Q000502:physiology*; D005780:Gelatin; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D011108:Polymers; D012710:Serum Albumin, Bovine; D012964:Sodium; D012995:Solubility; D012996:Solutions; D014867:Water
D000596:Amino Acids; D002241:Carbohydrates; D002414:Cations, Monovalent; D011108:Polymers; D012996:Solutions; D014867:Water; D012710:Serum Albumin, Bovine; D005780:Gelatin; D012964:Sodium