Neoplastic transformation of BALB 3T3 mouse embryo fibroblasts by the beef extract mutagen 2-amino-3-methylimidazo [4,5-d] quinoline.
The BALB 3T3 clone A31-1-1 mouse embryo fibroblast cell line at passage 7-13 was used for studying neoplastic transformation with a mutagen isolated from beef extract and identified as 2-amino-3 methylimidazo [4,5-d] quinoline (IQ). IQ at a concentration of 1, 5 and 15 ng/ml induced transformed type III foci. No foci were detected in control plates treated with DMSO alone. At the highest concentration of IQ (30 ng/ml) no transformed type III foci were observed and plating efficiency was reduced. The results demonstrate a positive transforming capability of this compound on mammalian cells.
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D005347:Fibroblasts; D008460:Meat; D051379:Mice; D008807:Mice, Inbred BALB C; D009153:Mutagens / Q000494:pharmacology*; D011804:Quinolines / Q000494:pharmacology*
D009153:Mutagens; D011804:Quinolines; C029216:2-amino-3-methylimidazo(4,5-f)quinoline