title: Use of alumina, organic solvent or ion-exchange columns to purify samples for determination of catecholamines in urine.
abstract: We present two methods for the determination of free adrenaline, noradrenaline and dopamine by liquid chromatography and electrochemical detection. The catecholamines are isolated from the urine sample either by organic solvent purification by ion-exchange column and followed in both cases by adsorption onto alumina. Analytical recoveries and CV of both methods are investigated. Many chromatograms of catecholamines in urine are presented.
mesh_terms: D000537:Aluminum Oxide; D002395:Catecholamines / Q000302:isolation & purification / Q000652:urine*; D002852:Chromatography, Ion Exchange; D006801:Humans; D012997:Solvents
chemical_list: D002395:Catecholamines; D012997:Solvents; D000537:Aluminum Oxide