De novo X-chromosome inactivation in somatic hybrid cells between the XO mouse embryonal carcinoma cell and XY rat lymphocyte.
Polyethylene glycol-mediated cell fusion between the hypoxanthine phosphoribosyl transferase (HPRT) deficient XO mouse embryonal carcinoma cell line, PSA-6TG1, and thymus or spleen lymphocytes from the normal male WKA/Hok rat gave rise to 35 somatic hybrid cultures. Hybrid cells being products of either a 1 : 1 or a 2 : 1 fusion invariably had morphological characteristics of endodermal cells from early embryos. BrdU-acridine orange (AO) fluorescence microscopy revealed do novo appearance of a late or early replicating, presumably genetically inactivated, mouse X chromosome in a substantial proportion of virtually tetraploid (XXY) or hexaploid (XXXY) hybrid cells.
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D004261:DNA Replication; D004303:Dosage Compensation, Genetic; D005260:Female; D006822:Hybrid Cells / Q000648:ultrastructure*; D007621:Karyotyping; D008214:Lymphocytes / Q000648:ultrastructure*; D008297:Male; D051379:Mice; D051381:Rats; D013724:Teratoma / Q000648:ultrastructure*; D014960:X Chromosome; D014998:Y Chromosome