[Culture of mouse embryo for the toxicological evaluation of drugs in vitro. (1). Establishment of in vitro culture system and effect of 6-mercaptopurine on development of early stage embryo].
The basal conditions for culture of early mouse embryo in vitro were examined for drug evaluation. Among the media tested, BMOC-III was most suitable for mouse embryo culture. Changing the medium at 1 or 3 hr after culture was harmful to the survival and/or development of early staged embryos, whereas at 12 or 24 hr after culture, changing of the medium showed slight effect on embryo development. Eight-cell staged embryos were more resistant to changing medium that two-cell staged ones. Preliminary work using a suitable culture system established that exposure to 6-mercaptopurine for 12 or 24 hr resulted in a prolonged developmental rate at each stage. The effects showed a dose-dependent relationship with the 24 hr exposure. It was suggested that inhibition of the developmental rate during exposure resulted from a direct toxic effect on the cell and that the inhibition after exposure resulted from metabolic toxicity.
D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D002970:Cleavage Stage, Ovum / Q000187:drug effects; D003470:Culture Media; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005260:Female; D015122:Mercaptopurine / Q000633:toxicity*; D051379:Mice; D011247:Pregnancy; D013997:Time Factors
D003470:Culture Media; D015122:Mercaptopurine