Use of radioimmunoassay procedures for the determination of sex hormones in animal tissues.
Radioimmunoassay methods for the determination of sex steroids and other compounds with sex hormone-like activities in various edible animal tissues and endocrine glands have been developed. Reliability of these methods, allowing quantification in a range of 10(-11) M, has been adequately demonstrated. In any case the necessary extraction procedures could be kept feasible as a result of the binding characteristics of the antisera available. When applied in respect to monitor for residues of anabolic sex hormones in edible tissues of veal calves, physiological baseline levels of some endogenous "anabolic" steroids (like testosterone, oestrogens) were established; in the case of xenobiotics residues at the scheduled time of slaughter could be quantified (trenbolone) and a regulatory method to implement the ban of diethylstilbestrol was introduced.
D000273:Adipose Tissue / Q000032:analysis; D000311:Adrenal Glands / Q000032:analysis; D000818:Animals; D002417:Cattle; D004054:Diethylstilbestrol / Q000032:analysis; D005260:Female; D012739:Gonadal Steroid Hormones / Q000032:analysis*; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D008460:Meat; D009132:Muscles / Q000032:analysis; D010053:Ovary / Q000032:analysis; D011863:Radioimmunoassay / Q000379:methods; D013739:Testosterone / Q000032:analysis; D014204:Trenbolone Acetate / Q000032:analysis
D012739:Gonadal Steroid Hormones; D013739:Testosterone; D004054:Diethylstilbestrol; D014204:Trenbolone Acetate