[Uric acid determination in dilute serum with an enzyme electrochemical and enzyme-free sensor].
The paper describes the development of electrochemical methods for analytical determination of uric acid basing on amperometric sensors: with an uricase enzyme electrode, termed "electrochemical-enzymatic" method; with an enzyme-free electrode by indication of the anodic oxidation of uric acid, termed "electrochemical" method. The comparison of analytical quality of both methods shows a sufficient specificity, precision, and accuracy. They show a good correlation with regard to the specific uricase-catalase reference method (UCM): yelectr./enz. = (0.943 xUCM + 19.8) mu mol/l; r = 0.9948 yelectr. = (0.964 xUCM + 6.31) mu mol/l; r = 0.9917 The analysis by the "electrochemical" method can be done with a higher frequency of samples per hour completely without need of enzyme. Both methods are principally suitable for an application in analytic biochemical laboratories.
D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized; D006801:Humans; D014503:Urate Oxidase; D014527:Uric Acid / Q000097:blood*
D004800:Enzymes, Immobilized; D014527:Uric Acid; D014503:Urate Oxidase
Harnsäurebestimmung in verdünntem Serum mit enzymelektrochemischem und enzymlosem sensor.