title: In vitro activation of dacarbazine (DTIC) for a human tumor cloning system.
abstract: Dacarbazine (DTIC) is an agent with clinical activity against human malignant melanoma. We have explored two methods for activating DTIC so it may be used in vitro in a human tumor cloning system. The activation of DTIC by white light was found to be a viable alternative to utilizing a microsome plus cofactor system for bioactivation. The microsome plus cofactor system itself actually caused some inhibition of tumor colony formation. Light activation of DTIC appears to be a reliable and simple method which allows testing of DTIC in an in vitro soft agar culture system.
mesh_terms: D000818:Animals; D001711:Biotransformation; D002460:Cell Line; D002999:Clone Cells; D003606:Dacarbazine / Q000378:metabolism / Q000528:radiation effects / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical; D006801:Humans; D007939:Leukemia L1210 / Q000188:drug therapy*; D008027:Light; D008545:Melanoma / Q000188:drug therapy*; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology
chemical_list: D003606:Dacarbazine