Regulation of methionine-enkephalin precursor messenger RNA in rat striatum by haloperidol and lithium.
Daily injections of rats with haloperidol for 21 days or lithium chloride for 5 days elevated the content of Met-enkephalin in the striatum by 86% or 66%, respectively. Poly(A)+ RNA from striata of drug-treated and control rats was translated in vitro, and the amount of synthesized enkephalin precursor [35S]preproenkephalin A was determined by immunoprecipitation and SDS-polyacrylamide gel electrophoresis. Haloperidol or lithium treatment increased the relative amount of preproenkephalin A synthesized by 67-98% or 25-29%, respectively. These results suggest that haloperidol elevates the Met-enkephalin content primarily by increasing the precursor mRNA content or activity, while lithium exerts its effect only in part by this mechanism.
D000818:Animals; D002474:Cell-Free System; D003342:Corpus Striatum / Q000378:metabolism*; D004744:Enkephalin, Methionine / Q000096:biosynthesis* / Q000235:genetics; D004745:Enkephalins / Q000378:metabolism*; D006220:Haloperidol / Q000494:pharmacology*; D008094:Lithium / Q000494:pharmacology*; D008297:Male; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344
D004745:Enkephalins; D011498:Protein Precursors; D012333:RNA, Messenger; D004744:Enkephalin, Methionine; C033660:preproenkephalin; D008094:Lithium; D006220:Haloperidol