Processing of prodynorphin at single and paired basic residues in porcine neurointermediate lobe.
The [Leu]enkephalin-containing peptides derived from prodynorphin have been quantitated in porcine neurointermediate lobe. Dynorphin-17, beta-neo-endorphin, [Leu]enkephalin, rimorphin and dynorphin-24 were found in highest concentrations (200-700 pmol/gm). No detectable amounts of the carboxyl terminal-extended rimorphin sequence (rimorphin 1-29) were observed, indicating that in addition to processing at paired basic residues, cleavage at the single Arg residue following rimorphin must be a major step in the processing of prodynorphin. Processing at Arg-Arg and/or Arg-Lys sequences to yield free [Leu]enkephalin also occurs to an appreciable extent in this tissue.
D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004723:Endorphins / Q000302:isolation & purification; D004745:Enkephalins / Q000235:genetics* / Q000302:isolation & purification; D010902:Pituitary Gland / Q000378:metabolism*; D011498:Protein Precursors / Q000235:genetics* / Q000302:isolation & purification; D013552:Swine
D004723:Endorphins; D004745:Enkephalins; D011498:Protein Precursors; C033660:preproenkephalin