MIR: a versatile program for the statistical analysis of enzyme kinetic data.
We have developed a package program for the estimation of Michaelis-Menten parameters for enzymes that conform to different kinetic mechanisms. Data from different experimental schemes can be fitted with appropriate weighing factors to any of 6 mathematical models, corresponding to 5 kinetic mechanisms: ordered bi-bi, Theorell-Chance, rapid equilibrium random bi-bi, rapid equilibrium ordered bi-bi and ping pong bi-bi. The program also performs a significance test to discriminate between different candidate models. To illustrate the performance of the program, real data from kinetic experiments with glucose 6-phosphate from Leuconostoc mesenteroides have been fitted to different mathematical models, and the results are discussed. The program can be easily implemented for the fitting of kinetic data to any other model.
D003201:Computers; D004798:Enzymes / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D005958:Glucosephosphates / Q000378:metabolism; D007700:Kinetics; D007934:Leuconostoc / Q000201:enzymology*; D012044:Regression Analysis; D012984:Software
D004798:Enzymes; D005958:Glucosephosphates; D005954:Glucosephosphate Dehydrogenase