Studies on transformation markers and tumorigenicity in segregant clones from a human hybrid line.
Thirty segregant clones were back-selected in 8AG or 5BUdR media from a non-tumorigenic human intraspecific hybrid line (HeLa TK- X fibroblasts HPRT-) displaying a high plasminogen activator (PA) level, a disorganized fibronectin (FN) matrix and anchorage-independence. These clones exhibited a widely modulated expression of the above markers concomitantly with different degrees of chromosome loss. Out of six representative segregant clones tested in nude mice, two were found to re-express tumorigenicity. No significant correlation was observed between PA or FN levels and anchorage-independence, as well as between these markers and tumorigenicity.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D002875:Chromosomes; D002999:Clone Cells / Q000378:metabolism / Q000648:ultrastructure; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D009368:Neoplasm Transplantation; D010960:Plasminogen Activators / Q000378:metabolism
D005353:Fibronectins; D010960:Plasminogen Activators