Detection of acyl-CoA beta-oxidation enzymes in peroxisomes (microperoxisomes) of mouse heart.
Homogenate of mouse heart was analyzed by using rate-dependent banding followed by density-dependent banding in sucrose density gradients held in zonal rotors. This protocol allowed substantial separation of myocardial microperoxisomes from other organelles. Particulate acyl-CoA oxidase activity was localized in microperoxisomes, while beta-hydroxyacyl-CoA dehydrogenase, carnitine acetyltransferase, carnitine octanoyltransferase and probably enoyl-CoA hydratase were partially localized in these organelles with most of the activity residing in the mitochondria. The specific activity of acyl-CoA oxidase in the peak microperoxisome fraction was at least 1/3 to 1/4 of that in highly purified renal or hepatic peroxisomes from mouse.
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D000214:Acyl Coenzyme A / Q000378:metabolism*; D042962:Acyl-CoA Oxidase; D000818:Animals; D002333:Carnitine Acyltransferases / Q000378:metabolism; D002332:Carnitine O-Acetyltransferase / Q000378:metabolism; D002458:Cell Fractionation; D004746:Enoyl-CoA Hydratase / Q000378:metabolism; D005260:Female; D008297:Male; D051379:Mice; D008830:Microbodies / Q000201:enzymology*; D008928:Mitochondria / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism
D000214:Acyl Coenzyme A; D010088:Oxidoreductases; D015094:3-Hydroxyacyl CoA Dehydrogenases; D042962:Acyl-CoA Oxidase; D002333:Carnitine Acyltransferases; C030162:carnitine octanoyltransferase; D002332:Carnitine O-Acetyltransferase; D004746:Enoyl-CoA Hydratase