title: Cytoskeletal organization in locomoting cells of the V2 rabbit carcinoma.
abstract: The relationship between the organization of cytoskeletal elements and locomotory activity was studied in single cells of the V2 rabbit carcinoma. Like migratory fibroblasts, and unlike colony-forming epithelial cells, these cells show a pronounced horizontal polarization, and develop a large lamella at their leading front. With affinity-purified antibodies and a combination of light and electron microscopic techniques, actin and alpha-actinin (but not myosin and tropomyosin) were found highly concentrated within the marginal region of the leading lamella, both in ruffles and in the underlying zone of contacts with the substratum. Close contacts prevailed in the locomotory cells and small focal contacts developed only in cells detaching from others. Focal contacts always contained small microfilament bundles. Reorganization of actin filaments is suggested as the fundamental event for the dynamic contact formation of the leading lamella. Large microfilament bundles (stress fibers) were absent in all stages of locomotion. Since locomotory behavior and shape changes of V2 cells are the same on glass as on the surface of a natural membrane, the rabbit mesentery, organization and distribution of contractile elements of cultured V2 cells probably reflect the in vivo situation.
mesh_terms: D000185:Actinin / Q000032:analysis; D000199:Actins / Q000032:analysis; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D002465:Cell Movement; D003599:Cytoskeleton / Q000032:analysis / Q000648:ultrastructure*; D008871:Microvilli / Q000648:ultrastructure; D011817:Rabbits; D014412:Tumor Virus Infections
chemical_list: D000199:Actins; D000185:Actinin