A lipopolysaccharide-specific bacteriophage for Aeromonas salmonicida.
Cell wall lipopolysaccharide (LPS) was identified as the receptor for the Aeromonas salmonicida bacteriophage strain 55R-1. Mutants of A. salmonicida resistant to phage 55R-1 were unable to adsorb phage 55R-1 and were shown to be defective in LPS structure. Purified A. salmonicida LPS inactivated phage 55R-1, but the O-polysaccharide and the core oligosaccharide portions of the LPS were ineffective. These results suggest that lipid A was required for receptor activity. Antibodies directed against LPS also inhibited phage adsorption.
D000333:Aeromonas / Q000032:analysis*; D001425:Bacterial Outer Membrane Proteins; D001435:Bacteriophages / Q000502:physiology*; D002473:Cell Wall / Q000032:analysis; D008070:Lipopolysaccharides / Q000502:physiology; D008565:Membrane Proteins / Q000032:analysis; D008970:Molecular Weight; D010750:Phosphoproteins / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis; D011991:Receptors, Virus / Q000032:analysis*
D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D010750:Phosphoproteins; D011135:Polysaccharides, Bacterial; D011991:Receptors, Virus