The dependence of fluid secretion by mandibular salivary gland and pancreas on extracellular calcium.
Acetylcholine-stimulated fluid secretion from the perfused rabbit mandibular salivary gland was inhibited in a biphasic manner when extracellular calcium concentration was reduced in the range 5 X 10(-4) - 10(-5)M. An initial rapid inhibition was followed by partial recovery to a plateau, the level of which depended upon the calcium concentration. Since no recovery was observed during substitution of calcium by strontium, recovery may depend upon an increased membrane permeability to calcium. It is concluded that acetylcholine evokes fluid secretion in this gland by enhancing calcium entry from the extracellular space, an action which can be mimicked by the calcium ionophore A23187. Changes in the electrolyte composition of saliva during calcium-depletion were such as to suggest that ductal reabsorption of sodium and chloride, and secretion of potassium are inhibited as extracellular calcium concentration is reduced. Secretin-stimulated fluid secretion from the cat pancreas was unaffected when perfusate calcium concentration was reduced to 2.5 X 10(-6)M and carbachol-stimulated amylase secretion was only slightly reduced. Since the latter is a calcium-dependent process, the source of calcium is presumably intracellular. In both glands, reducing calcium to 1 X 10(-6)M caused rapid and irreversible inhibition of fluid secretion.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002415:Cats; D004305:Dose-Response Relationship, Drug; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits; D012463:Saliva / Q000187:drug effects / Q000378:metabolism; D012633:Secretin / Q000494:pharmacology; D013324:Strontium / Q000378:metabolism; D013363:Submandibular Gland / Q000187:drug effects / Q000378:metabolism*
D012633:Secretin; D000109:Acetylcholine; D002118:Calcium; D013324:Strontium