title: The nucleotide sequence of cDNA complementary to the C1 component of rat prostatic binding protein.
abstract: The mRNA for component C1 of rat prostatic binding protein has been cloned and characterized. A partially purified mRNA fraction for this complex protein was reverse-transcribed into double-stranded cDNA and cloned into the PstI site of plasmid pBR 322. The 426-base-pair insert of the recombinant plasmid pC1A75 was completely sequenced. The coding region corresponds precisely to the 88 amino acid residues of C1 and in addition contains the information of a signal peptide of 23 residues. The 5' non-coding region counts only 19 nucleotides and is incomplete but the 3'-terminal non-coding part of 60 nucleotides extends into the poly(A) tail. Sequence analysis of other C1-positive clones indicates the presence of sequence rearrangements which must have occurred during the cloning procedure. Possible mechanisms for the generation of these cloning artefacts are discussed.
mesh_terms: D000727:Androgen-Binding Protein / Q000235:genetics*; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D055598:Chemical Phenomena; D002621:Chemistry; D003001:Cloning, Molecular; D004247:DNA; D008297:Male; D009693:Nucleic Acid Hybridization; D010446:Peptide Fragments / Q000235:genetics*; D011467:Prostate / Q000378:metabolism*; D060789:Prostatein; D012333:RNA, Messenger / Q000302:isolation & purification*; D051381:Rats; D060765:Secretoglobins; D014598:Uteroglobin; D060788:Mammaglobin B
chemical_list: D000727:Androgen-Binding Protein; D002352:Carrier Proteins; D004247:DNA; D010446:Peptide Fragments; D060789:Prostatein; D012333:RNA, Messenger; D060765:Secretoglobins; D014598:Uteroglobin; C511484:Scgb1d2 protein, rat; C511483:Scgb1d4 protein, rat; C556468:Scgb2a1 protein, rat; D060788:Mammaglobin B