Nitrite loss and spoilage microflora development in chub-packed luncheon meat.
Residual nitrite was lost from chub-packed luncheon meat during storage through both chemical breakdown and microbial consumption. The relative importance of these mechanisms in this pasteurized product was determined by the speed of development of the spoilage microflora, which is influenced by storage conditions. The nitrite half-life due to chemical loss was 13 d at 25 degrees C and 36 d at 10 degrees C. When microbial growth occurred these half-lives were reduced to 2.6 d and 21 d, respectively. Qualitative differences in the microflora that developed at these two temperatures (denitrifying Bacillus spp. at 25 degrees C and non-denitrifying Streptococcus spp. at 10 degrees C) account for the large temperature effect. Growth of Streptococcus spp. increased the rate of chemical nitrite loss in chubs by reducing the pH value. Nitrite did not inhibit the aerobic growth of either Bacillus or Streptococcus species associated with spoilage but did inhibit the anaerobic growth of Bacillus spp. This bacteriostatic effect of residual nitrite in anaerobic conditions will decrease during storage as nitrite level falls and oxygen penetrates the chub pack. Nitrite-mediated bacteriostasis does not obviate the need for refrigerated storage but does afford a real, if ephemeral, safeguard against spoilage occurring during short periods of temperature abuse.
D000818:Animals; D001407:Bacillus / Q000302:isolation & purification / Q000378:metabolism; D005511:Food Handling; D005516:Food Microbiology; D005520:Food Preservatives / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D009573:Nitrites / Q000032:analysis*; D013291:Streptococcus / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature
D005520:Food Preservatives; D009573:Nitrites