A simplified method for measuring activity of beta-D-fructofuranoside fructohydrolase (invertase).
A coupled enzymatic method for the assay of invertase is described. In this method, the fructose produced from sucrose by invertase action is converted to D-sorbitol by sorbitol dehydrogenase. The reaction is monitored by the decrease in A340 mm due to the consumption of NADH. The technique is simple, sensitive, and accurate, and compares well with alternative methods which rely on determination of glucose formed in the invertase reaction.
D000818:Animals; D002417:Cattle; D005948:Glucose Dehydrogenases; D006026:Glycoside Hydrolases / Q000032:analysis*; D007064:L-Iditol 2-Dehydrogenase; D008099:Liver / Q000201:enzymology; D012756:Sheep; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D043324:beta-Fructofuranosidase
D005948:Glucose Dehydrogenases; D007064:L-Iditol 2-Dehydrogenase; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase