title: Acceleration of cytokinesis in PtK1 cells treated with microtubule inhibitors.
abstract: Mitotic PtK1 cells were treated at furrow initiation with microtubule poisons to determine the role of microtubules in the regulation of terminal cytokinetic events. The administration of anti-microtubule agents in late anaphase accelerated the rate of cytokinesis by approx. 60% as measured by changes in furrow diameter. Application of colcemid, nocodazole, or vinblastine sulfate to cells at furrow initiation all increased the rate of furrowing. Nocodazole, applied at various concentrations, demonstrated a dose-dependent relationship with furrowing rate. These results suggest a coupling between the disorganization and depolymerization of microtubules and the acceleration of furrowing. Electron microscopic analysis of cells treated with microtubule inhibitors show approx. 70% fewer microtubule profiles in the constricted region between the daughter cells. Microtubules may play a restraining role in the rate of furrowing.
mesh_terms: D000705:Anaphase; D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003599:Cytoskeleton / Q000648:ultrastructure; D003703:Demecolcine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D007614:Macropodidae; D008870:Microtubules / Q000502:physiology* / Q000648:ultrastructure; D015739:Nocodazole; D014747:Vinblastine / Q000494:pharmacology*
chemical_list: D001562:Benzimidazoles; D014747:Vinblastine; D015739:Nocodazole; D003703:Demecolcine