A microradioiodination method for detection of nanogram amounts of proteins resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
A procedure is described for the rapid analysis of nanogram quantities of protein resolved by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). Proteins are first radioiodinated by the chloramine T method, acid precipitated after addition of a visible marker protein and collected on a nitrocellulose filter. The region of the filter containing protein is excised and the proteins are then extracted from the filter into electrophoresis sample buffer.
D001345:Autoradiography; D002700:Chloramines; D004591:Electrophoresis, Polyacrylamide Gel; D007457:Iodine Radioisotopes; D007467:Iodoproteins / Q000138:chemical synthesis*; D007553:Isotope Labeling / Q000379:methods*; D014105:Tosyl Compounds; D014462:Ultrafiltration
D002700:Chloramines; D007457:Iodine Radioisotopes; D007467:Iodoproteins; D014105:Tosyl Compounds; C016300:chloramine-T