[Effect of Staphylococcus aureus alpha-hemolysin on blood platelets from various animals].
Staphylococcal-alpha-hemolysin caused aggregation and subsequent lysis of blood platelets (BP) from cattle, pigs and rabbits. These reactions could be demonstrated by microtiter-plate-assay (Fig. 1) and aggregometry (Fig. 2). With the use of the latter, monophasic (by aggregation ans lysis) transmission curves were registered with BP in platelet-enriched plasma (PRP) from rabbits and with washed BP-suspensions from cattle, pigs and rabbits. Biphasic (by aggregation, desaggregation, reaggregation and lysis) transmission curves were formed with BP in PRP's from cattle and pigs. Prior addition of anti-alpha-hemolysin completely inhibited the effects of alpha-hemolysin on the BP. The reactions of the BP leading to the transmission curves in the aggregometer, could be confirmed by electron microscopy (Fig. 3) and by the release of 14C-serotonin from labelled BP (Fig. 4).
D000818:Animals; D001427:Bacterial Toxins / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology* / Q000648:ultrastructure; D002417:Cattle / Q000097:blood; D006460:Hemolysin Proteins / Q000276:immunology*; D006461:Hemolysis; D008854:Microscopy, Electron; D010974:Platelet Aggregation; D011817:Rabbits / Q000097:blood; D013552:Swine / Q000097:blood
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; C021091:staphylococcal alpha-toxin
Einwirkungen von alpha-Hämolysin von Staphylococcus aureus auf Blutplättchen verschiedener tierartlicher Herkunft.