Permeable embryonic cells of sea urchins as a model for studying nucleus-cytoplasm interactions.
Sea urchin embryonic cells were made permeable by treating them with glycerol solution for the purpose of exchanging cytoplasmic components. When proteoglycans prepared from embryos of advanced stages of development permeated into these glycerinated cells, these substances accumulated rapidly in the nucleus, being bound with chromatin.
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D002467:Cell Nucleus / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D005260:Female; D019793:Fluorescein; D005452:Fluoresceins; D005990:Glycerol / Q000494:pharmacology; D008954:Models, Biological; D011509:Proteoglycans / Q000378:metabolism; D012617:Sea Urchins / Q000196:embryology*; D015053:Zygote / Q000648:ultrastructure
D005452:Fluoresceins; D011509:Proteoglycans; D005990:Glycerol; D019793:Fluorescein