In vitro stimulated peripheral blood lymphocytes from multiple sclerosis patients produce idiotypes of oligoclonal CSF IgG.
Anti-idiotypic (anti-Id) antisera prepared against IgG from the cerebrospinal fluid of patients with multiple sclerosis were used to analyze culture supernatants of in vitro stimulated peripheral blood lymphocytes (PBL) from the patients. In the culture supernatants of pokeweed mitogen-stimulated PBL of four patients, idiotype-positive IgG was found. This finding provides additional evidence that the production of oligoclonal IgG in multiple sclerosis is not exclusively a feature of the central nervous system, but is also a systemic immunologic phenomenon.
D000921:Antibody-Producing Cells / Q000276:immunology; D000961:Antilymphocyte Serum / Q000276:immunology; D001667:Binding, Competitive; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid* / Q000145:classification; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis* / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D009103:Multiple Sclerosis / Q000276:immunology*; D010950:Plasma Cells / Q000276:immunology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology / Q000276:immunology
D000961:Antilymphocyte Serum; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D011043:Pokeweed Mitogens