Stabilization of human prostatic acid phosphatase by cross-linking with diamines.
The acid phosphatase [EC 3.1.3.2] from human prostate gland is very unstable glycoprotein. To stabilize the enzyme cross-linking reaction with diamines was adopted. The carboxyl groups of the enzyme were activated with 1-ethyl-(3-dimethylaminopropyl)-carbodiimide and then treated with diamines of H2N-(CH2)n-NH2 type. The modified enzyme with 1,12-dodecamethylenediamine preserved about 80% of its original activity and showed enhanced thermostability. Gel filtration and SDS-polyacrylamide gel electrophoresis showed the formation of cross-linked products with original molecular weight 100 KD and higher enzymatically active species.
D000135:Acid Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D001667:Binding, Competitive; D003432:Cross-Linking Reagents / Q000494:pharmacology; D003959:Diamines / Q000494:pharmacology*; D004355:Drug Stability; D004789:Enzyme Activation / Q000187:drug effects; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D011467:Prostate / Q000201:enzymology*
D003432:Cross-Linking Reagents; D003959:Diamines; C016417:1,12-dodecamethylenediamine; D000135:Acid Phosphatase; D005022:Ethyldimethylaminopropyl Carbodiimide