The biosynthesis of dentine gamma-carboxyglutamic acid-containing proteins by rat incisor odontoblasts in organ culture.
Rat odontoblasts were shown to synthesize and secrete gamma-carboxyglutamic acid(Gla)-containing proteins into dentine after organ culture in the presence of radiolabelled amino acid precursors. Purified dentine Gla-containing protein from rat incisors was used as antigen to prepare rabbit antisera as a probe of dentine Gla-containing-protein biosynthesis in organ cultures of dentine (rat incisor) and bone (rat calvaria). Use of the antiserum also pointed out the cross-reactivity of a high-M, glycoprotein present within the dentine matrix. The present results are significant in identifying dentine gla-containing protein as endogenous to mineralizing dentine and may relate to the commonality between calcifying connective tissues in general.
D015055:1-Carboxyglutamic Acid / Q000378:metabolism*; D000818:Animals; D002850:Chromatography, Gel; D003804:Dentin / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005971:Glutamates / Q000378:metabolism*; D007180:Incisor; D009804:Odontoblasts / Q000378:metabolism*; D009924:Organ Culture Techniques; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification; D051381:Rats
D005971:Glutamates; D011506:Proteins; D015055:1-Carboxyglutamic Acid