Vertical ascending electrophoresis of cells with a minimal stabilizing medium.
Vertical fractionation of a mixture of fixed horse and human red blood cells layered over a stabilizing support medium was done to give a valid comparison with proposed space experiments. In particular, the effects of sample thickness and concentration on zone migration rate were investigated. Electrophoretic mobilities of horse and human cells calculated from zone migration rates were compatible with those obtained by microelectrophoresis. Complete cell separation was observed when low power and effective cooling were employed.
D000818:Animals; D001799:Blood Sedimentation; D002465:Cell Movement; D002469:Cell Separation / Q000295:instrumentation*; D004574:Electromagnetic Fields; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D006736:Horses; D006801:Humans