[Effect of fatty acids on calmodulin regulation of adenylate cyclase from the brain striatal system].
p3eincubation of striatal membranes for 1 hour at 37 degrees C in the presence of calmodulin results in thermal inactivation of the calmodulin-insensitive adenylate cyclase; the activity of the calmodulin-sensitive form of the enzyme remains practically unchanged. Using a membrane preparation of striatal adenylate cyclase possessing a high sensitivity to calmodulin, the effects of fatty acids on regulation of adenylate cyclase by Ca2+ and calmodulin were studied. It was found that linolenic and palmitic acids exert a weak activating effect on adenylate cyclase in the presence of Ca2+, but do not practically affect the enzyme in Ca2+ free media. Linolenic acid almost completely inhibits the activation of adenylate cyclase by calmodulin; palmitic acid has no such effect. It was assumed that unsaturated fatty acids may prevent the interaction of adenylate cyclase with calmodulin.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology*; D002462:Cell Membrane / Q000201:enzymology; D003342:Corpus Striatum / Q000201:enzymology*; D005227:Fatty Acids / Q000494:pharmacology*; D007700:Kinetics; D011817:Rabbits
D002147:Calmodulin; D005227:Fatty Acids; D000262:Adenylyl Cyclases; D002118:Calcium
Vliianie zhirnykh kislot na reguliatsiiu kal'modulinom adenilattsiklazy striarnoĭ sistemy mozga.