Pteroylpolyglutamates in liver of phenobarbitone-treated rats.
The effects of "acute" phenobarbitone treatment on some aspects of folate metabolism in rat liver, that is total folate activity, protein bound and free folate, and various-length pteroylpolyglutamate amounts, were studied. The administration of two doses of the anticonvulsant drug (8 mg/100 g of body weight), caused a severe depletion in folate pool, free folate and in the percentage of longer-chain polyglutamates, while the amount of protein bound folates was unchanged. Such modifications are more evident in the animals killed at 24 h after the last drug injection rather than in those killed 12 h later. The possible mechanisms by which phenobarbitone might interfere with the synthesis of pteroylpolyglutamates, the actual coenzymic forms of folic acid, are discussed.
D000818:Animals; D005492:Folic Acid / Q000031:analogs & derivatives* / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D010634:Phenobarbital / Q000494:pharmacology*; D011624:Pteroylpolyglutamic Acids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D011624:Pteroylpolyglutamic Acids; D005492:Folic Acid; D010634:Phenobarbital