Purification of three rat atrial natriuretic factors and their amino acid composition.
A natriuretic factor has been described in the specific granules of rat atria. We have purified three factors which seem to be low-Mr peptides. They have been purified by means of acid extraction, octadecyl Sep-Pak cartridges, and chromatography on Bio-Gel P-10, CM Bio-Gel A, Mono S and reverse-phase high-performance liquid chromatography columns. The factors contain 26, 31 and 33 amino acids and may have been partially degraded during isolation. They are all 3 biologically active and the shorter one is the most active with a specific activity of 450 000 units/mg.
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005260:Female; D006325:Heart Atria / Q000032:analysis; D008297:Male; D009206:Myocardium / Q000032:analysis*; D045283:Natriuretic Agents; D011506:Proteins / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains
D000596:Amino Acids; D045283:Natriuretic Agents; D011506:Proteins