title: Fatty acid composition and primer specificity of de novo fatty acid synthetase in Bacillus globispores, Bacillus insolitus, and Bacillus psychrophilus.
abstract: The fatty acid compositions of three psychrophilic species of Bacillus were determined by gas--liquid chromatography. The proportions of straight-chain fatty acids, branched-chain fatty acids, and unsaturated fatty acids were found to be 13.3, 86.7, and 26.1% of the total cellular fatty acids for Bacillus globispores, 36.6, 63.4, and 25.1% for Bacillus insolitus, and 6.9, 93.1, and 18.4% for Bacillus psychrophilus, respectively. In all three organisms the de novo fatty acid synthetase specificity towards acyl-CoA primers was butyryl-CoA greater than propionyl-CoA much greater than acetyl-CoA. This shows that B. insolitus, which has an unusually large proportion of straight-chain fatty acids for Bacillus, does not possess a different de novo fatty acid synthetase than the other two organisms. Therefore, the greater proportion of straight-chain fatty acids in B. insolitus may be explained by a large supply of straight-chain primer.
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology*; D064429:Fatty Acid Synthases / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis* / Q000096:biosynthesis; D008316:Malonyl Coenzyme A / Q000378:metabolism
chemical_list: D000214:Acyl Coenzyme A; D005227:Fatty Acids; D008316:Malonyl Coenzyme A; D064429:Fatty Acid Synthases