Occurrence of short-sized oligo(A) fragments during course of cell cycle and ageing.
Affinity chromatography of nucleic acids precipitated by N-cetyl-N,N,N-trimethyl ammonium bromide on poly(U)-Sepharose has proved to be a suitable method for a nearly quantitative isolation of oligo(A) sequences down to a chain length of 4 nucleotide units. Analysis of short oligo(A) fragments in synchronized L5178y mouse lymphoma cells after labeling with [3H]Ado revealed that the percentage of A2-6 sequences on the total radioactivity amounted in S-phase cells to 1.6%, while the value obtained for the stationary L-cell system was 8.0%. The alterations of occurrence and chain length distribution of short oligo(A) fragments during ageing were studied in two age groups of female quails: mature (250-320 days old) and senescent animals (3-3.5 yr old). It was found that the amount of low molecular weight oligo(A) fragments gradually decreases during ageing of the animals; the amount in the mature animal group was significantly higher (6-fold) than in the old animal group. The decreased amounts of oligo(A) during S phase and ageing could in part be due to posttranslational modification of enzymes involved in poly(A) metabolism. It could be demonstrated that both homogeneous poly(A) anabolic poly(A) polymerase and homogeneous poly(A) catabolic endoribonuclease IV are phosphorylated by nuclear protein kinase NI.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000375:Aging; D000818:Animals; D002453:Cell Cycle; D002478:Cells, Cultured; D005260:Female; D007399:Interphase; D007940:Leukemia L5178; D051379:Mice; D008970:Molecular Weight; D009841:Oligonucleotides / Q000378:metabolism*; D009843:Oligoribonucleotides / Q000378:metabolism*; D010057:Oviducts; D010766:Phosphorylation; D011061:Poly A / Q000378:metabolism; D011784:Quail; D012333:RNA, Messenger / Q000302:isolation & purification
D000227:Adenine Nucleotides; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D012333:RNA, Messenger; D011061:Poly A; C023505:2',5'-oligoadenylate