[Identification of coenzyme B12 and other natural cobalamins by polarography].
AdoCbl, MeCbl and OH-Cbl were studied by methods of polarography and three-angle scan voltammetry. Cobalamines studied show a specific electrochemical activity that can be taken as a basis for their identification after separation and purification. The high sensitivity of the method allows to carry out the quantitative analysis of AdoCbl at concentrations ranging from 10(-6) M to 10(-4) M.
D003038:Cobamides / Q000032:analysis*; D004563:Electrochemistry; D011048:Polarography / Q000295:instrumentation / Q000379:methods*; D012996:Solutions; D014805:Vitamin B 12 / Q000032:analysis*
D003038:Cobamides; D012996:Solutions; D014805:Vitamin B 12
Identifikatsiia kofermenta B12 i nekotorykh drugikh prirodnykh kobalaminov metodom poliarografii.