How to prevent losses of protein by adsorption to glass and plastic.
An improved procedure for reducing the loss of protein by adsorption to glass or plastic surfaces is reported. For working with proteins at the microgram level, the solvent is modified by adding glycerol (50% final concentration) or Triton X-100 (0.2 mM final concentration). Coating the plastic or glass surfaces with proteins such as bovine serum albumin or other materials is not as effective; adding proteins such as bovine serum albumin to the solvent is counterproductive.
D000327:Adsorption; D003402:Creatine Kinase; D005898:Glass; D005990:Glycerol; D008156:Luciferases; D017830:Octoxynol; D010969:Plastics; D011092:Polyethylene Glycols; D011506:Proteins; D012997:Solvents; D013499:Surface Properties
D010969:Plastics; D011506:Proteins; D012997:Solvents; D011092:Polyethylene Glycols; D017830:Octoxynol; D008156:Luciferases; D003402:Creatine Kinase; D005990:Glycerol