[Serum fractionation by gel filtration at a high flow rate: use for diagnostic purposes].
High flow rates permit drastic reduction of time required in human serum fractionation by gel filtration giving relatively pure IgM. Fractionation of human serum on Bio-Gel A5m, Superose 6B and Sephacryl S-400, packed in 0.9 X 30 cm columns, at flow rate of 24 cm/h gives IgM fractions employable in diagnosis, in one hour time only.
D000180:Acrylic Resins; D000914:Antibodies, Viral / Q000032:analysis*; D002850:Chromatography, Gel / Q000379:methods*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D006882:Hydroxyapatites; D007075:Immunoglobulin M / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D008623:Mercaptoethanol; D012409:Rubella / Q000097:blood*; D012685:Sepharose; D012698:Serologic Tests / Q000379:methods*
D000180:Acrylic Resins; D000914:Antibodies, Viral; D006882:Hydroxyapatites; D007075:Immunoglobulin M; D007136:Immunoglobulins; C037239:Sephacryl Superfine; D008623:Mercaptoethanol; C043127:Superose 6b; D012685:Sepharose
Frazionamento del siero mediante gel-filtrazione con elevate velocita' di flusso: utilizzazione a scopo diagnostico.