X chromosome-linked transmission and expression of retroviral genomes microinjected into mouse zygotes.
A genomic clone consisting of the Moloney leukemia proviral genome with moderately repetitive mouse sequences was microinjected into the pronucleus of a mouse zygote. An animal was derived that carried multiple copies of proviral DNA in a tandem array. No evidence for homologous recombination was obtained. The viral genome was expressed in this animal and was transmitted as a single unit to its offspring. Subsequent breeding studies revealed that the proviral DNA had integrated on an X chromosome.
D000818:Animals; D002467:Cell Nucleus / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation; D005814:Genes, Viral; D051379:Mice; D008845:Microinjections; D008979:Moloney murine leukemia virus / Q000235:genetics*; D011995:Recombination, Genetic; D012730:Sex Chromosomes / Q000502:physiology*; D014960:X Chromosome / Q000502:physiology*