A simple and efficient method for solubilization of the dopamine receptor.
The solubilization of dopamine receptors from bovine caudates was carried out with different detergents and detergent-salt combination. 0.25 M cholic acid: 1 M NaCl combination solubilized more effectively than the other detergents used. Solubilized receptors demonstrated the similar affinity and specificity as the membrane receptors. Solubilized receptors satisfied all the criteria of solubilization. Solubilized receptors were highly stable at 4 degrees C and freezing at -70 degrees C.
D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D002421:Caudate Nucleus / Q000032:analysis*; D011954:Receptors, Dopamine / Q000302:isolation & purification*; D012995:Solubility; D013570:Synaptic Membranes / Q000032:analysis
D011954:Receptors, Dopamine