Inhibition of dopamine beta-hydroxylase by 2-mercapto-1-methylimidazole.
The inhibition of bovine dopamine beta-hydroxylase (dopamine beta-monooxygenase, EC 1.14.17.1) by 2-mercapto-1-methylimidazole has been studied using a simple, 'metal-free' assay system. 2-Mercapto-1-methylimidazole is an uncompetitive inhibitor of dopamine beta-hydroxylase with respect to ascorbate and a mixed type of inhibitor with respect to tyramine. These findings are consistent with 2-mercapto-1-methylimidazole interacting exclusively with the reduced form of dopamine beta-hydroxylase.
D000311:Adrenal Glands / Q000201:enzymology*; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002417:Cattle; D002614:Chelating Agents / Q000494:pharmacology; D004299:Dopamine beta-Hydroxylase / Q000037:antagonists & inhibitors*; D007700:Kinetics; D008713:Methimazole / Q000494:pharmacology*; D012117:Resins, Synthetic; D014439:Tyramine
D002614:Chelating Agents; D012117:Resins, Synthetic; C024997:Chelex 100; D008713:Methimazole; D004299:Dopamine beta-Hydroxylase; D001205:Ascorbic Acid; D014439:Tyramine