Enzymatic methylation of inverted DNA repeats in the vicinity of the mouse beta-major globin gene.
This paper examines the extent of enzymatic methylation in 5'-CCGG sequences of inverted repeats in DNA isolated from adult liver and bone marrow of DBA/2 mice, with special attention to the methylation of such sequences in the vicinity of the beta-major globin gene. Two thirds of inverted repeats contain 5'-AGCT and 5'-CCGG sequences, as found by a method based on the capability of inverted repeats of forming intramolecular duplexes under the conditions of "zero-time" reassociation. Methylation in internal cytosines of 5'-CCGG sequences of inverted DNA repeats differs between bone marrow and liver tissues. The beta-major globin gene was found in DNA covalently linked to inverted repeats. The enzymatic methylation of inverted repeats neighbouring the beta-major globin gene differs at HpaII recognition sites; the DNA of bone marrow tissue, in which this gene is expressed, is less methylated at such sites as compared to liver DNA.
D000818:Animals; D001853:Bone Marrow / Q000032:analysis; D004247:DNA / Q000378:metabolism*; D005796:Genes; D005914:Globins; D008099:Liver / Q000032:analysis; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D051379:Mice; D008811:Mice, Inbred DBA; D008967:Molecular Biology; D012091:Repetitive Sequences, Nucleic Acid
D005914:Globins; D004247:DNA; D008780:Methyltransferases