title: Eosinophil chemotactic factor in schistosome eggs: a comparative study of eosinophil chemotactic factors in the eggs of Schistosoma japonicum and S. mansoni in vitro.
abstract: Significant chemotactic activity for eosinophils was detected in soluble egg antigen (SEA) preparations of both Schistosoma japonicum and S. mansoni in dose-dependent fashion. The activity of S. japonicum SEA was higher than that of S. mansoni SEA. Gel filtration on Sephadex G-150 showed that S. japonicum SEA was composed of two groups of eosinophil chemotactic factors (ECFs), one of high molecular weight (JEE-H) and the other of low molecular weight (JEE-L). S. mansoni SEA showed ECF composition similar to that of S. japonicum SEA. JEE-H was stable on heating (100 degrees C, 60 min) and resistant to pronase digestion, but was sensitive to periodate oxidation. JEE-L was also stable on heating and resistant to pronase and carboxypeptidase A digestions. These properties of the ECFs were also held in common with those of S. mansoni SEA. JEE-L was extractable by toluene, indicating a hydrophobic nature. These results suggest that schistosome eggs themselves contain ECFs, and that the composition of S. mansoni and S. japonicum SEA-derived ECFs is essentially the same. However, they differ from the other ECFs which have already been described in schistosome infections.
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D002630:Chemotactic Factors / Q000302:isolation & purification*; D002631:Chemotactic Factors, Eosinophil / Q000302:isolation & purification*; D002850:Chromatography, Gel; D004803:Eosinophilic Granuloma / Q000503:physiopathology; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D010063:Ovum / Q000502:physiology*; D012549:Schistosoma japonicum / Q000502:physiology*; D012550:Schistosoma mansoni / Q000502:physiology*; D012552:Schistosomiasis / Q000503:physiopathology
chemical_list: D000941:Antigens; D002630:Chemotactic Factors; D002631:Chemotactic Factors, Eosinophil