A quantitative assay for ciliate chemotaxis.
A quantitative bioassay for ciliate chemotaxis based on the capillary principle is described using Tetrahymena thermophila as test organism. The attractant-containing assay tube designed for the bioassay attracts up to 4 X 10(4) cells in 2 h which makes electronic cell counting of the chemotactic response feasible. The attractants used are solutions of proteose peptone and yeast extract which also are growth media for this organism.
D002452:Cell Count / Q000295:instrumentation*; D002633:Chemotaxis; D004581:Electronics; D010461:Peptones; D013768:Tetrahymena / Q000502:physiology*; D013997:Time Factors
D010461:Peptones