Cultivation of rat cerebellar cells under conditions of optimal oxygen supply.
A method is described for the cultivation of essentially small cerebellar neurones under optimal oxygen supply. Cerebellar cells were seeded onto polylysine-coated dishes equipped with a gas-permeable bottom (Petriperm). Under these conditions, cells migrated to form small groups and developed dense networks of fibres covering the entire bottom of the dish. Contamination with non-neuronal cells was restricted to fibroblasts (less than 0.1%), oligodendrocytes (less than 1%) and astrocytes (approximately 6%), even after prolonged cultivation (15 days).
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002531:Cerebellum / Q000166:cytology*; D046508:Culture Techniques; D009457:Neuroglia / Q000166:cytology; D009474:Neurons / Q000166:cytology; D010101:Oxygen Consumption; D051381:Rats