Modification of the slide agglutination test for the detection of sperm-agglutinins.
We have modified the slide agglutination test (SAT) in order to make it available for the routine detection and titration of sperm agglutinins. Serum and seminal plasma samples of 156 subfertile patients were tested by the modified slide agglutination test (MSAT) in two trials. Fifteen per cent of serum and 7.5% of siminal plasma samples gave positive results and MSAT showed excellent reproducibility: a highly significant statistical correlation between the positive titres in the two determinations was observed. One hundred and ten samples were tested simultaneously by the MSAT and the tray agglutination test (TAT). The two tests showed a similar sensitivity with a significant statistical correlation between the positive titres. Using the MSAT the reading of results was easier and more precise. Therefore we propose the MSAT for the routine detection and titration of sperm-agglutinins.
D006801:Humans; D007248:Infertility, Male / Q000276:immunology; D008297:Male; D008722:Methods; D012661:Semen / Q000032:analysis / Q000276:immunology; D013073:Sperm Agglutination; D013094:Spermatozoa / Q000032:analysis / Q000276:immunology