Separation and quantitative determination of radiolabeled prostaglandins, thromboxanes, 6-keto-prostaglandin F1 alpha and other arachidonic acid metabolites produced in biological material.
Evaluation of several thin-layer chromatographic procedures for the separation of various labeled arachidonic acid metabolites (including 6-keto-prostaglandin F1 alpha) produced in the biological system is described. Manual scanning and autoradiography of the plates developed by two-dimensional thin-layer chromatography was also done for locating the radioactivities due to arachidonic acid metabolites other than thromboxane B2 and the classical prostaglandins (PGF2 alpha, PGE2, and PGD2).
D015121:6-Ketoprostaglandin F1 alpha / Q000302:isolation & purification; D000818:Animals; D001011:Aorta / Q000378:metabolism; D001095:Arachidonic Acids / Q000302:isolation & purification*; D001345:Autoradiography; D001792:Blood Platelets / Q000378:metabolism; D002855:Chromatography, Thin Layer; D006801:Humans; D066298:In Vitro Techniques; D008168:Lung / Q000378:metabolism; D011453:Prostaglandins / Q000302:isolation & purification*; D011457:Prostaglandins D / Q000302:isolation & purification; D011458:Prostaglandins E / Q000302:isolation & purification; D011460:Prostaglandins F / Q000302:isolation & purification; D051381:Rats; D013929:Thromboxane B2 / Q000302:isolation & purification*; D013931:Thromboxanes / Q000302:isolation & purification*
D001095:Arachidonic Acids; D011453:Prostaglandins; D011457:Prostaglandins D; D011458:Prostaglandins E; D011460:Prostaglandins F; D013931:Thromboxanes; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha