title: Differentiation of Trichinella spiralis spiralis and Trichinella spiralis nativa based on resistance to low temperature refrigeration.
abstract: A refrigeration technique to differentiate the subspecies, Trichinella spiralis spiralis and T. spiralis nativa is described. Trichinella spiralis spiralis trichinae in musculature do not survive 48 hours post-refrigeration at -32 degrees C while T. spiralis nativa will survive 72 hours and longer at the same temperature.
mesh_terms: D000818:Animals; D005289:Ferrets / Q000469:parasitology; D005589:Foxes / Q000469:parasitology; D009132:Muscles / Q000469:parasitology; D051381:Rats / Q000469:parasitology; D012034:Refrigeration; D012376:Rodent Diseases / Q000469:parasitology; D014234:Trichinella / Q000145:classification*; D014235:Trichinellosis / Q000469:parasitology / Q000662:veterinary*; D001503:Ursidae / Q000469:parasitology