[Comparison of 2 extraction methods of immunoglobulins A and serum albumin in sputum].
The concentrations of immunoglobulins A (IgA) and serum albumin (SA) determined in sputum by electro-immunoassay have been compared after the extraction of proteins by two methods: 1) ion exchange chromatography and 2) mechanical agitation at high ionic strength. No significant difference of IgA is observed between the two methods. On the contrary, a very significant decrease in the concentration of SA is observed by the chromatographic extraction which is incomplete when pH - value of extract is lower than that of the isoelectric point of SA.
D002852:Chromatography, Ion Exchange; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay; D007070:Immunoglobulin A / Q000032:analysis / Q000302:isolation & purification*; D012709:Serum Albumin / Q000032:analysis / Q000302:isolation & purification*; D013183:Sputum / Q000032:analysis*
D007070:Immunoglobulin A; D012709:Serum Albumin
Comparaison de deux méthodes d'extraction des immunoglobulines A et de la sérumalbumine dans l'expectoration.