title: New method for determination of mast cells degranulation in vitro. Effect of preimplantation embryos on mast cells.
abstract: A new method has been developed for determination of the degranulating activity of substances present in small volumes and exhibiting a low degranulating potency. By the use of this method MC degranulation was tested, induced by preimplantation of mouse embryos. It was found that old morulae and young blastocysts (4th day of pregnancy) contain a factor which reacts with MC causing extrusion of cytoplasmic grains. It is suggested that degranulation activity may be connected with the formation of a new, biologically active compounds thus with the biochemical differentiation of the embryos.
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000276:immunology; D004622:Embryo, Mammalian / Q000276:immunology*; D047108:Embryonic Development; D005089:Exocytosis / Q000187:drug effects; D005260:Female; D008407:Mast Cells / Q000276:immunology*; D009028:Morula / Q000276:immunology; D011247:Pregnancy; D011479:Protamines / Q000494:pharmacology; D051381:Rats
chemical_list: D011479:Protamines