title: Radioiodination studies of the envelopes from Xenopus laevis eggs.
abstract: To investigate the molecular basis of the observed morphological and biological characteristics of coelomic egg envelopes (CE), vitelline envelopes (VE), and fertilization envelopes (FE) of Xenopus laevis eggs, envelopes were radioiodinated under a variety of conditions: in situ, isolated and intact, or solubilized. The distribution of 125I in envelope components was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Each envelope type displayed unique profiles when iodinated in the intact state. A major constituent of VE, the 41,500 molecular weight component, was not labeled in the intact state, although the corresponding component of CE was heavily labeled. After dissociation of the envelope by guanidine-HCl or sodium dodecyl sulfate, all of the components could be radioiodinated. However, when the envelopes (VE and FE) were dissolved by heating and subsequently radioiodinated by lactoperoxidase, the resulting radioactivity profile was similar to that of the intact envelopes, suggesting that in the heat-dissolved envelope, the individual components retain similar structural relations as in the intact envelope. Quantitative but not qualitative differences were found between the inner and outer aspects of VE and FE. The significance of these findings is discussed in relation to what is known about the morphological, biological, and molecular properties of the envelopes.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006146:Guanidines / Q000494:pharmacology; D007457:Iodine Radioisotopes; D007784:Lactoperoxidase / Q000378:metabolism; D008970:Molecular Weight; D010063:Ovum / Q000032:analysis / Q000648:ultrastructure*; D014817:Vitelline Membrane / Q000032:analysis; D014982:Xenopus laevis
chemical_list: D006146:Guanidines; D007457:Iodine Radioisotopes; D007784:Lactoperoxidase