Binding studies on two different monoclonal antibodies raised against CEA.
Binding characteristics of two mouse monoclonal antibodies MA/1 and MA/200 raised to carcinoembryonic antigen (CEA) are presented. Double antibody radioimmunoassay and a solid phase assay confirmed that MA/1 binds weakly to CEA extracted from tumour tissue but strongly to CEA in patients' serum. Antibody MA/200 binds with high affinity to both 'tumour' and 'serum' CEA. Ligand-receptor relationship for MA/1 and MA/200 binding to both tumour CEA and CEA isolated from serum are presented from equilibrium binding data analysed by the method of Scatchard. Co-titration experiments also indicated that MA/1 and MA/200 and an established polyclonal anti-CEA bind to a similar subpopulation of CEA or CEA-like molecules, but the binding data demonstrate that their respective binding sites are chemically different.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D003110:Colonic Neoplasms / Q000276:immunology; D006801:Humans; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen