[Interference of hemolysis in plasma urea determination. A comparison of 2 methods: classical Berthelot urease and the modified Berthelot urease].
The authors have tested the interference of the hemoglobin by two routine methods ( Berthelot classic and Berthelot modified) for the determination of plasmatic urea. From their work it appears that Berthelot classic method already presents a very sensible positive interference for hemoglobin at 0.04 g/dl level while for the Berthelot modified method such interference begins to be significant only for those values which are than 1.25 g/dl level. The correlation between these two methods, tested by limpid and lacking in hemolysis plasmas, is very good, so the authors think the Berthelot modified method is more respondent to the exigence of the routine as it is not exceptional the case to have to execute anyhow some determinations of urea and plasmas which have a partial hemolysis.
D005069:Evaluation Studies as Topic; D006454:Hemoglobins / Q000032:analysis; D006461:Hemolysis; D006801:Humans; D008722:Methods; D014508:Urea / Q000097:blood*; D014510:Urease / Q000097:blood*
D006454:Hemoglobins; D014508:Urea; D014510:Urease
Interferenza dell'emolisi sulla determinazione dell'urea plasmatica. Confronto tra due metodiche: ureasi-Berthelot classica ed ureasi-Berthelot modificata.