Ultrastructural localization of DNA in tissue culture cell nuclei by means of enzyme-gold and autoimmune sera-protein A-gold techniques.
The post-embedding localization of DNA was investigated in cell nuclei by means of DNase I-gold and autoimmune sera-protein A-gold techniques. Using the former technique, gold particles were found mainly over euchromatin, nucleoli exhibit occasionally high labeling. In the latter technique, the use of the serum binding dsDNA confined the label mainly to condensed chromatin.
D000199:Actins; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure*; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000032:analysis*; D005260:Female; D006046:Gold; D006367:HeLa Cells / Q000648:ultrastructure; D006801:Humans; D007106:Immune Sera; D007202:Indicators and Reagents; D008854:Microscopy, Electron; D010053:Ovary; D013205:Staphylococcal Protein A
D000199:Actins; D007106:Immune Sera; D007202:Indicators and Reagents; D013205:Staphylococcal Protein A; D006046:Gold; D004247:DNA