High-performance liquid-chromatographic method for the determination of 4'-(9-acridinylamino)methanesulfon-m-anisidide in plasma.
A rapid and selective high-performance liquid chromatographic method for the measurement of 4'-(9-acridinylamino)methanesulfon-m-anisidide (AMSA), a new anticancer drug, has been developed. The method employed an analogue of AMSA, 4'-(3-methyl-9-acridinylamino)methanesulphonanilide as internal standard. Plasma samples were acidified, washed with hexane, readjusted to pH 9.0 and extracted with diethyl ether. The evaporated extract was chromatographed on a Radial-Pak C18 column using acetonitrile--water containing 0.01 mol/l triethylamine phosphate as mobile phase. Detection was by UV absorbance at 254 nm. Chromatography time for each sample was 5.5 min. Using 0.5 ml of plasma, AMSA concentrations as low as 50 nmol/l could be measured with acceptable accuracy and precision. Patients samples remained stable when stored at -20 degrees C for up to one month. Plasma AMSA concentrations were followed for 24 h after 200 mg/m2 infusions in two patients with acute myeloid leukemia. This method appears eminently suitable for investigation of the pharmacokinetics of AMSA in patients and laboratory animals.
D000609:Aminoacridines / Q000097:blood*; D000677:Amsacrine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D013048:Specimen Handling; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000609:Aminoacridines; D000677:Amsacrine