Repair in arterial tissue. An ultrastructural demonstration of proteoglycans in low temperature embedded normal and healing arterial tissue.
Low temperature embedded normal and healing arterial tissues were studied by transmission electron microscopy. The most conspicuous observation was the ultrastructurally well-preserved extracellular matrix of the arterial tissue. The proteoglycans were visible as rod-like structures (20-50 nm in width and about 200 nm in length) and radiating filaments connecting the granules to each other. The rod-like structures were removed after testicular hyaluronidase digestion. Chondroitinase ABC digestion removed most of the rod-like structures. Re-endothelialized neointima revealed a high content of rod-like structures forming a three-dimensional muralium joining together basal membrane microfibrils, collagen microfibrils and the microfibrillar component of elastin, whereas non-re-endothelialized neointima showed a reduced content of proteoglycans. The cylindrical granules may represent the proteoglycan monomer with condensed glycosaminoglycan chains.
D000818:Animals; D001013:Aorta, Thoracic / Q000293:injuries / Q000648:ultrastructure*; D002810:Chondroitinases and Chondroitin Lyases; D005109:Extracellular Matrix; D005615:Freezing; D006821:Hyaluronoglucosaminidase; D008297:Male; D008854:Microscopy, Electron; D011509:Proteoglycans / Q000032:analysis*; D011817:Rabbits; D014021:Tissue Preservation; D014945:Wound Healing
D011509:Proteoglycans; D006821:Hyaluronoglucosaminidase; D002810:Chondroitinases and Chondroitin Lyases