Nuclear protein changes following N,N-dimethylformamide (DMF)-induced maturation.
A human colonic carcinoma cell line was exposed to a nontoxic concentration of N,N-dimethylformamide (DMF) for 2 wk. Nuclear proteins were isolated from control and treated cells and compared by sodium dodecyl sulfate (SDS) electrophoresis. Qualitative and quantitative differences were observed. Metabolic labeling with tritiated leucine demonstrated qualitative variation between control and treated cells.
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D003110:Colonic Neoplasms; D004126:Dimethylformamide / Q000494:pharmacology*; D006801:Humans; D007930:Leucine / Q000378:metabolism; D009698:Nucleoproteins / Q000378:metabolism*; D014158:Transcription, Genetic
D009698:Nucleoproteins; D004126:Dimethylformamide; D007930:Leucine