title: Characterization of an antigen present on testicular cells and preimplantation embryos whose expression is modified by the t12 haplotype.
abstract: In attempts to identify cell surface molecules specified by lethal genes in the T/t-complex, we prepared a rabbit antiserum that has cytotoxic activity against testicular cells from males heterozygous for t12, but not against wild type cells. However, anti-t12 serum immunoprecipitates the same major component, a glycoprotein of mol. wt. 87,000 daltons, from galactose-labelled C3H. +/t12 testicular cell lysate and from congenic C3H. +/+ lysate, although the gp87 molecule precipitated from +/t12 cells appears to be more highly galactosylated than the +/+ form. The antigen is heavily glycosylated in both genotypes, since when testicular cells are treated with tunicamycin before immunoprecipitation, a protein of 40,000-42,000 daltons is obtained. Gp87 is also present on pre-implantation embryos, and on teratocarcinoma cells, but is barely detectable on any adult somatic cells examined. Its expression is developmentally regulated during pre-implantation stages, but the temporal pattern of its expression appears to be different between wild type and t12 embryos. Thus, we believe we have identified a molecule that may play a role in the differentiation of testicular cells and pre-implantation embryos, and that is either specified by genes in the t12 haplotype, or responsive in some way to the effects of t12.
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D001755:Blastocyst / Q000276:immunology*; D002460:Cell Line; D002846:Chromatography, Affinity / Q000379:methods; D003602:Cytotoxicity, Immunologic; D005804:Genes, Lethal; D006579:Heterozygote; D037102:Lectins; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013724:Teratoma / Q000276:immunology; D013737:Testis / Q000276:immunology*; D014018:Tissue Distribution
chemical_list: D000954:Antigens, Surface; D037102:Lectins