title: High-performance liquid chromatography of phospholipids: quantitation by phosphate analysis.
abstract: Quantitation of individual phospholipids separated by HPLC from tissue extracts by colorimetric analysis of phosphate was investigated. Elution of inorganic phosphate and breakthrough of lecithin were determined using radioisotopes. A substance which interfered with sample phosphate determinations was found in the column eluant, and a method to minimize its effect was developed. This method allows accurate quantitation of individual phospholipids present at a minimum of 20 nmol phosphate.
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D008099:Liver / Q000032:analysis; D009411:Nerve Endings / Q000032:analysis; D010710:Phosphates / Q000302:isolation & purification*; D010743:Phospholipids / Q000302:isolation & purification*; D051381:Rats; D013574:Synaptosomes / Q000032:analysis
chemical_list: D010710:Phosphates; D010743:Phospholipids