[Fractionation of serum by gel filtration: contamination of IgM fractions by non-specific inhibitors of hemagglutination of the rubella virus].
Rubella virus hemagglutination non-specific inhibitors, in sera fractionated on Bio-Gel A 5 m, elute in IgM containing fractions. In addition, in heparin-MnCl2 treated sera, the same inhibitors display a different chromatographic behaviour on Bio-Gel A 5m, being eluted earlier than IgM. Therefore, in order to avoid false-positive results due to the presence of non-specific inhibitors in IgM containing fractions it is necessary to verify the disappearance of hemagglutination-inhibition activity after 2-ME reduction.
D001774:Blood Chemical Analysis / Q000379:methods*; D002850:Chromatography, Gel; D006385:Hemagglutination Inhibition Tests; D006389:Hemagglutinins, Viral / Q000032:analysis*; D006801:Humans; D007075:Immunoglobulin M / Q000302:isolation & purification*; D012412:Rubella virus
D006389:Hemagglutinins, Viral; D007075:Immunoglobulin M
Frazionamento del siero mediante gel-filtrazione: contaminazione delle frazioni delle IgM da parte degli inibitori non specifici dell'emagglutinazione da virus della Rosolia.