Concentrated, digestible DNA after hydroxylapatite chromatography with cetylpyridinium bromide precipitation.
A method for the direct extraction of the DNA from the unfavorable phosphate eluant of hydroxylapatite chromatography is described. The DNA--reversibly precipitated with the cationic detergent cetylpyridinium bromide--can be subjected to further enzymatic manipulations within minutes. This method is applied to the rapid separation of pBR322 plasmid from the chromosomal DNA.
D002594:Cetylpyridinium; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D002845:Chromatography / Q000379:methods*; D004247:DNA / Q000302:isolation & purification*; D006882:Hydroxyapatites; D010710:Phosphates; D010957:Plasmids; D011726:Pyridinium Compounds
D006882:Hydroxyapatites; D010710:Phosphates; D011726:Pyridinium Compounds; D004247:DNA; D002594:Cetylpyridinium