title: Retinol does not affect cell growth in fibroblast cell cultures.
abstract: Retinol-free fetal calf serum for cell cultures was obtained by UV irradiation and by removal of retinol-binding protein (RBP) by gel-filtration. RBP from bovine serum was purified in a simple two-step procedure. This made it possible to replenish the culture medium with retinol in its physiological i.e. RBP-bound form. Retinol-free and retinol-containing medium was used to assay growth of 3T3 and L929 fibroblast cells. None of these cell lines showed a significant change in growth rate or saturation density that could be attributed to free or RBP-bound retinol.
mesh_terms: D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012177:Retinol-Binding Proteins / Q000302:isolation & purification; D014801:Vitamin A / Q000494:pharmacology*
chemical_list: D012177:Retinol-Binding Proteins; D014801:Vitamin A