Detection of Clostridium difficile cytotoxin in HEp-2 and CHO cell lines.
The detection of Clostridium difficile cytotoxin was compared in two established cell lines; Chinese hamster ovary (CHO) and a human epithelial line, HEp-2. All specimens with positive toxin assays were detected with the CHO cell line, whereas only one-half of positive specimens were detected with the HEp-2 cells.
D000818:Animals; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000032:analysis*; D002460:Cell Line; D003013:Clostridium / Q000302:isolation & purification / Q000378:metabolism; D003015:Clostridium Infections / Q000382:microbiology; D006224:Cricetinae; D003412:Cricetulus; D003603:Cytotoxins / Q000032:analysis*; D004848:Epithelium; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D010053:Ovary
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003603:Cytotoxins; C031815:toxB protein, Clostridium difficile