Evidence of 2-hydroxylation of estradiol-17 beta 17-glucuronide by male rat liver microsomes.
When estradiol-17 beta 17-glucuronide was incubated with male rat liver microsomal preparations with a NADPH-generating system, 2-hydroxyestradiol-17 beta 17-glucuronide was obtained. This 2-hydroxylation was shown to occur without cleavage of the conjugate group. The result clearly indicates that estradiol-17 beta 17-glucuronide could act as substrate for rat liver microsomal 2-hydroxylase.
D000818:Animals; D019363:Cytochrome P-450 CYP1A1; D004958:Estradiol / Q000031:analogs & derivatives* / Q000378:metabolism; D006900:Hydroxylation; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013250:Steroid Hydroxylases / Q000378:metabolism
C025483:estradiol-17 beta-glucuronide; D004958:Estradiol; D013250:Steroid Hydroxylases; D019363:Cytochrome P-450 CYP1A1; C020120:estrogen 2-hydroxylase