Clones from cultured, B16 mouse-melanoma cells resistant to wheat-germ agglutinin and with altered production of mucin-type glycoproteins.
Several clones resistant to wheat-germ-agglutinin toxicity were isolated from B16 mouse-melanoma cells. The resistance may be explained in part by fewer binding sites for the agglutinin on the variant lines; the total, cellular sialic acid content appeared somewhat reduced. Analysis of cell glycoproteins indicated a decreased content of sialic acid in one glycoconjugate that binds to wheat-germ agglutinin and has O-linked oligosaccharides.
D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D004351:Drug Resistance; D006023:Glycoproteins / Q000096:biosynthesis*; D037102:Lectins / Q000494:pharmacology*; D008545:Melanoma / Q000503:physiopathology*; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D009077:Mucins / Q000096:biosynthesis*; D009374:Neoplasms, Experimental / Q000503:physiopathology; D009439:Neuraminidase; D012794:Sialic Acids / Q000032:analysis; D014909:Wheat Germ Agglutinins
D006023:Glycoproteins; D037102:Lectins; D008565:Membrane Proteins; D009077:Mucins; D012794:Sialic Acids; D014909:Wheat Germ Agglutinins; D009439:Neuraminidase