title: Simultaneous analysis of conformation and transcription of A and B groups of vitellogenin genes in male and female Xenopus during primary and secondary activation by estrogen.
abstract: In male Xenopus, primary estradiol administration results in noncoordinate activation in the liver of the A and B groups of vitellogenin genes, both as judged by transcription and DNase I sensitivity in isolated nuclei, B group genes being activated preferentially in the first 20 hr. Secondary induction in males or "primary" induction in females results in a coordinate and equal transcription of these two groups of genes. The elevated transcriptional activity following primary estrogen stimulation returns to low levels rapidly but the high DNase I sensitivity of these genes persists for 2-3 months. A non-coordinate activation of the A and B groups of vitellogenin genes is however re-established in response to a second administration of estradiol 8 months after primary stimulation of male Xenopus.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005796:Genes / Q000187:drug effects*; D007700:Kinetics; D008074:Lipoproteins / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D008297:Male; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012737:Sex Factors; D014158:Transcription, Genetic / Q000187:drug effects*; D014819:Vitellogenins / Q000235:genetics*; D014981:Xenopus
chemical_list: D008074:Lipoproteins; D014819:Vitellogenins; D004958:Estradiol; D004247:DNA