High-resolution autoradiography in mouse brain and cerebellum 10 days after radiolabelled CDP-choline administration.
A dose of 5 microCi of cytidine diphosphate choline (CDP-choline, citicoline, Somazina) (methyl 14C) was administered to mice. Animals were sacrificed 10 days after administration and their brain and cerebellum isolated for autoradiography and electron microscopy. Sections were prepared in such a manner that a general topographic assignment of radioactivity distribution to different layers and brain structures could be made. Regarding the intracellular distribution of the different cellular types observed, Purkinje's cells exhibited in a density of focus emission higher than the others.
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D002531:Cerebellum / Q000378:metabolism*; D002794:Choline / Q000031:analogs & derivatives*; D003566:Cytidine Diphosphate Choline / Q000378:metabolism*; D051379:Mice
D003566:Cytidine Diphosphate Choline; D002794:Choline