Rat liver mitochondrial aspartate aminotransferase mRNA: isolation of mRNA by polyacrylamide gel electrophoresis.
In order to obtain an enriched fraction of mRNA for mAAT we applied the following method: preparation of cytoplasmic polysomes; isolation of total RNA; isolation of mRNA by chromatography on oligo(dT)-cellulose column; polyacrylamide gel electrophoresis; extraction of mRNA from the gel. The procedure seems to allow isolation of mRNA enriched fraction for mAAT. An enriched fraction of mRNAs can be useful for the preparation of corresponding cDNAs.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D008930:Mitochondria, Liver / Q000201:enzymology*; D008970:Molecular Weight; D012333:RNA, Messenger / Q000302:isolation & purification*; D051381:Rats
D012333:RNA, Messenger; D001219:Aspartate Aminotransferases