Identification of subunits of RNA polymerase II from Ehrlich ascites tumor cells.
A procedure was developed for large scale purification of RNA polymerase II from Ehrlich ascites tumor cells. About 2 mg of purified enzyme was obtained from 800 g of wet cells. Ten subunits were identified which behaved corresponding to the enzyme activity both on DEAE-Sephadex chromatography and on glycerol density gradient centrifugation. Analysis of tryptic peptides of each subunit showed that these subunits were independent proteins and not structurally related to each other.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification*; D046911:Macromolecular Substances; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D012319:RNA Polymerase II / Q000302:isolation & purification*
D046911:Macromolecular Substances; D010446:Peptide Fragments; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases