title: Human proinsulin VI. Synthesis of protected segment 46-70 of prohormone.
abstract: The synthesis of the protected pentacosapeptide Trt-Gly-Gly-Pro-Gly-Ala-Gly-Ser-(But)-Leu-Gln-Pro-Leu-Ala-Leu-Glu( OBut)-Gly-Ser (But)-Leu-Gln-Lys(Boc)-Arg-Gly-Ile-Val-Glu-(OBut)-Gln-OH (Pos. 46-70) of human proinsulin is described. This segment was prepared by the mixed anhydride condensation of the trityl-protected peptides (46-64) or (46-59) with the fragments 65-70 and 60-70, respectively. In both the cases the purification was effected by counter current distribution in a yield of 25% and 24%, respectively.
mesh_terms: D000595:Amino Acid Sequence; D001322:Autoanalysis; D002849:Chromatography, Gas; D006801:Humans; D007202:Indicators and Reagents; D009904:Optical Rotation; D010446:Peptide Fragments / Q000138:chemical synthesis*; D011384:Proinsulin / Q000138:chemical synthesis*
chemical_list: D007202:Indicators and Reagents; D010446:Peptide Fragments; C040309:proinsulin (46-70); D011384:Proinsulin