title: Long-term culture of human oligodendrocytes. Isolation, growth and identification.
abstract: Oligodendrocytes were isolated from adult human brains obtained at autopsy. The cells were prepared by Percoll density gradient centrifugation, seeded on plastic coverslips and were cultured for a period up to 6 months. The oligodendrocytes in culture expressed cell-type specific markers, galactocerebroside and myelin basic protein and revealed the ultrastructure of mature oligodendrocytes.
mesh_terms: D000328:Adult; D000368:Aged; D001921:Brain / Q000473:pathology / Q000502:physiology*; D002455:Cell Division; D002469:Cell Separation; D002478:Cells, Cultured; D006801:Humans; D008854:Microscopy, Electron; D008875:Middle Aged; D009457:Neuroglia / Q000502:physiology*; D009836:Oligodendroglia / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure