The enhancement of reaggregation of sea urchin blastula cells by exogenous proteins.
The ability of exogenous proteins to enhance the reaggregation of aggregation-deficient butanol extracted cells has been studied using blastula cells of the sea urchin Arbacia punctulata. Butanol extracts, calcium- and magnesium-free sea-water dissociation supernatant, human plasma fibronectin and bovine serum albumin enhance reaggregation in a concentration dependent manner. Electrophoretic analysis and autoradiography of 125I surface labeled dissociated cells indicate that while butanol extracts a large number of proteins, few iodinated proteins are present in the butanol extract. The non-specificity of proteins to promote reaggregation and the probable cellular location of the butanol extracted proteins are discussed.
D000818:Animals; D000440:Butanols; D002118:Calcium / Q000502:physiology; D002449:Cell Aggregation; D008970:Molecular Weight; D011506:Proteins / Q000502:physiology; D012617:Sea Urchins / Q000166:cytology*
D000440:Butanols; D011506:Proteins; D002118:Calcium