Ribonucleotides are not channeled into DNA in permeabilized mammalian cells.
Reddy and Pardee earlier reported that permeabilized CHEF/18 cells (J. Biol. Chem 257, 12526) or soluble extracts from such cells (Cell 32, 443) under the proper conditions preferentially incorporated a labeled ribonucleotide (CDP) into DNA without dilution by an excess of non-labeled dCTP, suggesting channeling of ribonucleotides into DNA by a multiprotein complex. Here we present results suggesting that in these experiments CDP was incorporated into RNA and not into DNA.
D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability; D006224:Cricetinae; D003412:Cricetulus; D004261:DNA Replication; D004622:Embryo, Mammalian; D007700:Kinetics; D012265:Ribonucleotides / Q000378:metabolism*; D014316:Tritium
D012265:Ribonucleotides; D014316:Tritium