Sulphate turnover of surface proteoglycans in cultured rat smooth muscle cells.
Turnover of radioactive sulphate-labelled proteoglycans in cultured rat smooth muscle cells was detected by pulse chase techniques. The degradation appeared to take the form of desulphation of sulphated macromolecules, with a loss in total sulphate of approximately 50% in 5 days. The desulphation process occurred in the pericellular/matrix compartment of the culture system and was unaffected by inhibition of matrix formation by beta-aminopropionitrile, or by incubation of cells with lysomotropic inhibitors. There was no evidence for further degradation of desulphated species even when exogenous, radio-labelled proteoglycans were added to fresh cultures and incubated for four days. Labelled macromolecules initiated on xyloside acceptors were desulphated by rat smooth muscle cell cultures more slowly than intact proteoglycans.
D000818:Animals; D002478:Cells, Cultured; D002850:Chromatography, Gel / Q000379:methods; D003470:Culture Media; D006025:Glycosaminoglycans / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats; D013431:Sulfates / Q000378:metabolism*; D013462:Sulfur Radioisotopes
D003470:Culture Media; D006025:Glycosaminoglycans; D011509:Proteoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes