title: The polymorphism of the vitamin D-binding protein (Gc); isoelectric focusing in 3 M urea as additional method for identification of genetic variants.
abstract: Since the last report numerous new DBP (Gc) variants have been observed; at present a total of 84 different mutants can be distinguished. Several of them have similar electrophoretic mobilities and/or isoelectric points of conventional isoelectric focusing (IEF). IEF in polyacrylamide gels in the presence of 3 M urea is a convenient and efficient method for the detection of hidden variation.
mesh_terms: D002352:Carrier Proteins / Q000235:genetics*; D014644:Genetic Variation; D006801:Humans; D007525:Isoelectric Focusing; D010641:Phenotype; D011110:Polymorphism, Genetic; D014508:Urea / Q000032:analysis*; D014809:Vitamin D-Binding Protein
chemical_list: D002352:Carrier Proteins; D014809:Vitamin D-Binding Protein; D014508:Urea