title: Cooperative effects in the process of elution of Newcastle disease virus pre-attached on chick embryo cell monolayer culture.
abstract: After the virus attachment to the cell surface at 4 degrees C followed by incubation of the virus-loaded cells at 37 degrees C two processes are going simultaneously: one portion of the attached virions penetrates inside the cells and another portion elutes outside the cells (a certain portion during a certain time period remains bound to the cell surface). Quantities of both penetrated and eluted viruses depended on the quantity of the attached virus. The dependence of the quantity of the penetrated virus on the quantity of the attached virus was linear, while there was an accelerating increase of the quantity of the eluted virus depending on the quantity of the attached virus. This non-linearity is described by a mathematical model based on cooperativity effects which are caused by a certain interaction between the attached virions. Since, according to the calculations, this interaction is effective at the distance equal to about 15-fold virion diameter, a kind of "at distance" interaction between the adjacent virions realized through spreading modifications of plasma membrane is suggested.
mesh_terms: D000327:Adsorption; D000818:Animals; D002462:Cell Membrane / Q000382:microbiology; D002478:Cells, Cultured; D002642:Chick Embryo; D008954:Models, Biological; D009522:Newcastle disease virus / Q000254:growth & development / Q000302:isolation & purification*