Antigen binding with chromatin of immunocompetent cells.
Binding of [125I] IgG to chromatin of spleen cells was studied in vivo, in vitro and to isolated chromatin. In vivo, maximal saturation of chromatin occurred 1 day after immunization (1.3 X 10(12) molecules per spleen, 10(8) cells). At the same level the antigen bound to chromatin in vitro. Binding of [125I] IgG with isolated chromatin was dependent on antigen concentration.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000502:physiology*; D002843:Chromatin / Q000502:physiology*; D004705:Endocytosis; D005786:Gene Expression Regulation; D007136:Immunoglobulins / Q000235:genetics*; D008214:Lymphocytes / Q000502:physiology*; D051381:Rats
D000941:Antigens; D002843:Chromatin; D007136:Immunoglobulins