Prolactin-releasing activity of porcine intestinal peptide (PHI-27).
Porcine intestinal peptide (PHI), a twenty-seven amino acid peptide isolated from porcine gut extracts, is a close structural homolog of the secretin family hormones. The structural and biological similarities of PHI to vasoactive intestinal peptide (VIP) together with its presence in the rat hypothalamus suggested a possible role for the peptide in the control of prolactin (PRL) secretion. PHI induced significant, dose-related stimulations of PRL release from cultured, dispersed rat pituitary cells in vitro. The minimum effective dose is 10(-7) molar, compared to 10(-9) molar for VIP. No interactive effect with thyrotropin-releasing hormone was observed; however, PHI partially overcame the dopamine inhibition of PRL release.
D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005768:Gastrointestinal Hormones / Q000494:pharmacology*; D005934:Glucagon / Q000494:pharmacology; D008297:Male; D010451:Peptide PHI; D010455:Peptides / Q000494:pharmacology*; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012633:Secretin / Q000494:pharmacology; D013972:Thyrotropin / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D005768:Gastrointestinal Hormones; D010451:Peptide PHI; D010455:Peptides; D012633:Secretin; D014660:Vasoactive Intestinal Peptide; D011388:Prolactin; D013972:Thyrotropin; D005934:Glucagon