Lipases production by Bacillus circulans under mesophilic and osmophilic conditions. Factors affecting lipases production.
A facultative osmophilic bacterium able to produce lipases (isolated from bee honey) was identified as Bacillus circulans. Factors affecting lipases production by such isolate were studied. Dox's liquid medium supplied with 2 and/or 30% sucrose was used as a basal medium, under static culture condition, for such a purpose. Results have showed that the optimal sucrose concentration at which the isolate produced its maximal yield of lipases was 30% after an incubation period of 24 hours at 40 degrees C, and this was corresponding to an incubation period of 48 hours at 40 degrees C in presence of 2% sucrose. The optimal pH value was 7 in the first case while, 6.5 in the second. Furthermore production of extracellular lipases by such isolate was achieved in presence and absence of tributyrin as a carbon source instead of sucrose when supplied to Dox's liquid medium. Also the production of extracellular lipases by such isolate was not enhanced in presence of tributyrin as a supplement to Dox's liquid medium when supplied with 2 and/or 30% sucrose as carbon sources. This might suggest that the extracellular lipases of such isolate is constitutive in nature.
D001407:Bacillus / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000096:biosynthesis*; D009994:Osmolar Concentration; D013395:Sucrose / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D014280:Triglycerides / Q000494:pharmacology; D015002:Yeast, Dried / Q000494:pharmacology
D014280:Triglycerides; D013395:Sucrose; D008049:Lipase; C005830:tributyrin