Growth of 10 Leptospira interrogans serovars using polyvinylpyrrolidone(PVP)-treated Tween in protein-free medium.
Polyvinylpyrrolidone (PVP)-treated Tween was used to prepare a protein-free medium for cultivation of leptospires. 6 out of 25 media were selected for culturing 10 serovars of leptospires. The best results were achieved in a medium containing Tween treated with PVP 25000. In several subcultures 8 out of 10 serovars reached a concentration of 10(8) leptospires/ml or more, when cultured in tubes without aeration.l Only in the case of 2 serovars (Leptospira interrogans serovars grippotyphosa and tarassovi) growth was not satisfactory. The results have shown that it is possible to detoxify Tween with the aid of polyvinylpyrrolidone. In the meantime the 40th subculture of 8 strains has been prepared.
D003470:Culture Media; D007921:Leptospira interrogans / Q000254:growth & development*; D008970:Molecular Weight; D011136:Polysorbates; D011205:Povidone; D013045:Species Specificity
D003470:Culture Media; D011136:Polysorbates; D011205:Povidone