[Streptokinase immobilization in insoluble carriers, and properties of the immobilized protein].
Immobilization of streptokinase was performed by bromine cyan-activated cellulose and by aminoethyl cellulose using glutaric aldehyde and N-cyclohexyl-N'-[2-(4-morpholinyl)-ethyl-carbodiimide. The specific activator activity of the immobilized streptokinase is 70-100% of that of free streptokinase. In multiple application of the immobilized protein preparations streptokinase obtained by bromine cyan-activated cellulose is the most stable: it retains more than 40% of initial activity after 10 repeated applications. The immobilized streptokinase is shown to be more thermostable as compared to the soluble one.
D002482:Cellulose / Q000031:analogs & derivatives; D004800:Enzymes, Immobilized / Q000032:analysis*; D006801:Humans; D010960:Plasminogen Activators / Q000032:analysis; D012995:Solubility; D013300:Streptokinase / Q000032:analysis*; D013696:Temperature
D004800:Enzymes, Immobilized; D002482:Cellulose; D013300:Streptokinase; D010960:Plasminogen Activators
Immobilizatsiia streptokinazy na nerastvorimykh nositeliakh i svoĭstva immobilizirovannogo belka.