Identification of macrophages in sections of rabbit lung using acetoacetylated lipoproteins.
Macrophages were labeled in sections of rabbit lung with acetoacetylated low density lipoprotein (LDL), a marker internalized by cultured macrophages but not by other connective tissue cells. Using a modified technique, thin slices of fresh rabbit lung were incubated in 3,3'-dioctadecylindocarbocyanine (DiI)-labeled, acetoacetylated LDL, fixed in paraformaldehyde, and sectioned. Alveolar macrophages incorporated the fluorescently labeled, modified LDL, but surrounding stroma and parenchyma did not stain. Our results indicate that DiI-labeled, acetoacetylated LDL may be used to identify mononuclear phagocytes in tissue sections.
D000818:Animals; D002232:Carbocyanines / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008168:Lung / Q000032:analysis / Q000166:cytology*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000032:analysis / Q000166:cytology; D011804:Quinolines / Q000378:metabolism*; D011817:Rabbits
D002232:Carbocyanines; D008077:Lipoproteins, LDL; D011804:Quinolines; C024286:3,3'-dioctadecylindocarbocyanine