Citrate accumulation by a Golgi apparatus-rich fraction from lactating bovine mammary gland.
1. Golgi apparatus-rich fractions from lactating bovine mammary gland rapidly accumulated citrate from incubation medium. Characteristics of this process suggested that a citrate transport system may be present in Golgi apparatus membranes. 2. Endoplasmic reticulum fractions accumulated citrate at nearly the same rate as Golgi apparatus; secretory vesicle fractions displayed lower ability to accumulate citrate. Intact epithelial cells (acini) from lactating mammary gland did not accumulate citrate. 3. Citrate accumulation by Golgi apparatus was pH and temperature sensitive but was not altered by metabolic inhibitors. 4. These observations suggest a role for Golgi apparatus in packaging intracellular citrate for secretion into milk.
D000818:Animals; D002417:Cattle; D002951:Citrates / Q000378:metabolism*; D005260:Female; D006056:Golgi Apparatus / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008892:Milk / Q000032:analysis; D011247:Pregnancy
D002951:Citrates