FAB-mapping of recombinant-DNA protein products.
A new method is described for screening and establishing the primary structure of proteins. The procedure is both rapid and sensitive and allows study of the C-terminus of the protein with equal facility to the N-terminus. This new strategy which is illustrated here for the polypeptide hormone Insulin, has obvious applications in recombinant DNA biotechnology research, in post-translational modification and site-directed mutagenesis studies, or any other aspect of modern protein chemistry requiring accurate definition of primary structure.
D000595:Amino Acid Sequence; D004274:DNA, Recombinant / Q000378:metabolism*; D009154:Mutation; D010446:Peptide Fragments / Q000032:analysis; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000235:genetics*; D013057:Spectrum Analysis / Q000379:methods
D004274:DNA, Recombinant; D010446:Peptide Fragments; D011506:Proteins