Binding sites for [3H]gamma-hydroxybutyrate on cultured neurones of rat cerebellum and spinal cord.
By means of light microscopic autoradiography, binding sites for the GABA catabolite [3H]gamma-hydroxybutyrate (GHB) were observed on cultured cerebellar and spinal neurones but not on glial cells. [3H]GHB was bound to similar types of neurones as [3H]GABA. However, the number of neurones labelled by [3H]GHB was considerably smaller than that by [3H]GABA, and the intensity of labelling by [3H]GHB was usually weaker. It is suggested that GHB might interact with neurotransmission mediated by GABA or play a role as neuromodulator or neurotransmitter in the mammalian central nervous system.
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D012978:Sodium Oxybate / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D014018:Tissue Distribution
D006885:Hydroxybutyrates; D012978:Sodium Oxybate