H NMR detection of the specific change to the uncoupled state of the mitochondrial membrane.
The spin-lattice relaxation times T1 of H2O in mitochondrial suspensions at various energy states were measured at 28 degrees C. It was found that de-energization on the addition of an uncoupler of oxidative phosphorylation affects T1: after the addition of the uncoupler, T1 first decreases and then increases to a value greater than those at other different energy states. The T1 values of the unfrozen water in mitochondrial suspensions were measured at -15 degrees C after the energy state of the mitochondria was freeze-trapped. It was found that they increase only in the presence of an uncoupler. These changes in T1 are discussed in relation to the specific change of the mitochondrial membrane in the uncoupled state.
D000818:Animals; D004734:Energy Metabolism; D007425:Intracellular Membranes / Q000502:physiology; D009682:Magnetic Resonance Spectroscopy; D008930:Mitochondria, Liver / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D014475:Uncoupling Agents / Q000494:pharmacology; D014867:Water / Q000032:analysis
D014475:Uncoupling Agents; D014867:Water