Separation of both fibrous and globular proteins on the basis of molecular weight using high-performance size exclusion chromatography.
A high-performance size exclusion liquid chromatographic system has been used to separate proteins with different shapes solely on the basis of their molecular weights. After the effects of ionic and hydrophobic interactions with the stationary phase have been overcome, protein elution is normally governed by their effective size in solution. Conditions are described under which proteins, with isoelectric points within the normal operating pH range of the columns, are eluted independent of their Stokes' radii. Even fibrous proteins with axial ratios of 50 elute according to their known molecular weights over the range 2000-2,000,000.
D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007525:Isoelectric Focusing; D008970:Molecular Weight; D011489:Protein Denaturation; D011506:Proteins / Q000302:isolation & purification*
D011506:Proteins