Allotypic restriction of the expression of MOPC460 idiotope after immunization with either anti-2,4-dinitrophenyl (DNP) or anti-idiotypic antibodies.
The MOPC460 idiotype is expressed in mice with the IghCa allotypic haplotype after anti-2,4-dinitrophenyl (DNP) immunization. We have previously shown that two monoclonal syngeneic anti-idiotypic antibodies (IDM 92-13 and IDM 41-27) define two distinct idiotopes (the 460.92 and the 460.41) on the M460 idiotype. The current study demonstrates that only one idiotope (460.92) is recurrently expressed after antigen immunization in IghCa positive mice and also that, immunization against the monoclonal anti-idiotypic molecules induces the synthesis of 460.92 idiotope positive anti-DNP antibodies. However, the detection of such molecules is only possible when animals with the IghCa allotypic haplotype are immunized with the IDM 92-13 molecules. Immunization of mice with either of the two monoclonal anti-idiotypic antibodies never results in the synthesis 460.41 positive molecules. Therefore, whatever protocol of immunization used, the expression of 460.92 was allotypic restricted.
D019297:2,4-Dinitrophenol; D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D004140:Dinitrophenols; D007126:Immunoglobulin Allotypes / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D010954:Plasmacytoma / Q000276:immunology*; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D004140:Dinitrophenols; D007126:Immunoglobulin Allotypes; D007130:Immunoglobulin Idiotypes; D019297:2,4-Dinitrophenol