High-performance liquid chromatographic quantitation of trimethoprim, sulfamethoxazole, and N4-acetylsulfamethoxazole in body fluids.
We describe a rapid, precise and simple procedure for the quantitative determination of trimethoprim, sulfamethoxazole, and N4-acetylsulfamethoxazole in body fluids by reversed-phase high-performance liquid chromatography. This method utilizes antipyrine as an internal standard with the compounds detected by dual-wavelength monitoring at 225 nm and 254 nm after a single-step extraction. Precision, sensitivity, and accuracy of this assay are within the range of clinical utility; the coefficient of variation is less than or equal to 3%, sensitivity less than 0.5 micrograms/ml for all compounds, and recovery greater than 97%. The short time for performance and small sample size makes the assay ideal for clinical drug monitoring and pharmacokinetic studies.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D013420:Sulfamethoxazole / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood; D014295:Trimethoprim / Q000032:analysis* / Q000097:blood
C021282:N(4)-acetylsulfamethoxazole; D014295:Trimethoprim; D013420:Sulfamethoxazole