The quantitation of psilocybin in hallucinogenic mushrooms using high performance liquid chromatography.
A method using high performance liquid chromatography (HPLC) with an acetonitrile, water, and phosphoric acid mobile phase and a bonded cyano-amino-type polar phase column has been developed for the rapid, selective, and accurate quantitation of psilocybin in dry mushroom material. A simple one-step procedure is used for the quantitative extraction of psilocybin in under 60 min. The 267:254 nm absorbance ratio is used as a check on peak purity for the psilocybin response.
D001487:Basidiomycota / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D011562:Psilocybin / Q000032:analysis*
D011562:Psilocybin