Identification of fibronectin fragments that bind to carboxy-group-modified proteins.
Limited proteolysis of human plasma fibronectin with chymotrypsin, trypsin or thermolysin has been used to localize binding sites responsible for binding [Vuento, Korkolainen & Stenman (1982) Biochem. J. 205, 303-311] of fibronectin to carboxy-group-modified proteins. These bindings sites are different from those mediating binding of fibronectin to gelatin or heparin. They are located close to the C-terminus of the polypeptide chains of fibronectin, and apparently overlap with the C-terminal fibrin binding site.
D001665:Binding Sites; D002918:Chymotrypsin; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D011485:Protein Binding; D013820:Thermolysin; D014357:Trypsin
D005353:Fibronectins; D007074:Immunoglobulin G; D010446:Peptide Fragments; D002918:Chymotrypsin; D014357:Trypsin; D013820:Thermolysin