title: Interaction of hopanoids with phosphatidylcholines containing oleic and omega-cyclohexyldodecanoic acid in lipid bilayer membranes.
abstract: Lipid bilayer membranes were made from hopanoid phosphatidylcholine mixtures dissolved in decane. The specific capacity of the mixed membranes was found to increase with increasing hopanoid content. This indicates an interaction between hopanoids and lipids which leads to a reduction of the chemical potential of the solvent in the membranes. The structural properties of mixtures of hopanoids and phosphatidylcholines were investigated using charged probe molecules, the negatively charged lipophilic ions dipicrylamine (DPA) and tetraphenylborate (TphiB) and the positively charged potassium complex PV-K+ (PV, cyclo (D-Val-L-Pro-L-Val-D-Pro)3). The transport properties of the lipophilic ions in the mixed membranes indicate that the electrical properties like dipolar potential and surface potentials of phosphatidylcholine membranes are not changed by the insertion of the hopanoids. The translocation rate constant K of the PV-K+ complex is drastically reduced in the hopanoid phosphatidylcholine membranes with increasing hopanoid content. This effect is discussed on the basis of an alteration of the microviscosity in the mixed membranes. There exists a close analogy between the action of cholesterol and hopanoids in bilayer membranes from phosphatidylcholines. A bilayer membrane composed of di-omega-cyclohexyldodecanoyl-phosphatidylcholine (DCPC) was found to possess a higher specific capacity as compared to other phosphatidylcholines. Also a lower translocation rate constant for PV-K+ was observed which may be caused by the relative high microviscosity of this lipid even above the phase transition temperature.
mesh_terms: D001407:Bacillus; D003510:Cyclohexanes; D019605:Euryarchaeota; D006017:Glycolipids / Q000302:isolation & purification; D008051:Lipid Bilayers; D008563:Membrane Lipids; D008567:Membranes, Artificial; D019301:Oleic Acid; D009829:Oleic Acids; D010713:Phosphatidylcholines; D013329:Structure-Activity Relationship; D014315:Triterpenes
chemical_list: D003510:Cyclohexanes; D006017:Glycolipids; D008051:Lipid Bilayers; D008563:Membrane Lipids; D008567:Membranes, Artificial; D009829:Oleic Acids; D010713:Phosphatidylcholines; D014315:Triterpenes; C040447:omega-cyclohexyldodecanoic acid; D019301:Oleic Acid; C025206:hopane