beta-D-Mannosidase from human placenta: properties and partial purification.
beta-D-Mannosidase was partially purified (108-fold) from human placenta by ammonium sulfate precipitation, Concanavalin A-Sepharose affinity chromatography, gel filtration and hydroxylapatite chromatography. Further attempts to purify the enzyme by several conventional methods failed due to loss of activity. The stability of beta-mannosidase, the effect of several compounds on its activity and some physico-chemical and kinetic parameters were investigated.
D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D007700:Kinetics; D008361:Mannosidases / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D044902:beta-Mannosidase
D008361:Mannosidases; D044902:beta-Mannosidase