[Basic characteristics of Gunma clone-4 in the experimental study of metastasis].
Gunma clone-4 (Gc-4) cell was isolated by limiting dilution method from Radiological Science-Chiba- Toyama sarcoma which appeared spontaneously in a C3H/He mouse and cultured for generations. The character of Gc-4 cells was as follows: Gc-4 cells grew exponentially in vivo and in vitro, and the doubling time was 3.4 to 4.2 days in vivo and 20 to 22 hours in vitro respectively. Inoculation of 1 X 10(6) Gc-4 cells into A/Jackson mice and C57BL/6 mice resulted in rejection and disappearance within 17 days. Inoculation of 1 X 10(6) Gc-4 cells into C3H/He mice which were immunized with Gc-4 cells treated with 100 micrograms/ml of mitomycin C for 30 min. did not result in rejection. The Gc-4 cells showed histiocytic and fibroblastic characteristics morphologically and functionally, and corresponded to human malignant fibrous histiocytoma. Experiments on artificial and natural metastasis of Gc-4 cells revealed that Gc-4 cells had a high colony forming efficiency.
D000818:Animals; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002999:Clone Cells / Q000166:cytology; D006084:Graft Rejection; D008175:Lung Neoplasms / Q000556:secondary; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D012513:Sarcoma, Experimental / Q000276:immunology / Q000473:pathology* / Q000556:secondary