Synthesis of oligonucleotides containing the hypermodified base, alpha-putrescinylthymine.
Self-complementary decadeoxyribonucleotides containing alpha-putrescinyl-thymidine (Put Thd), AAGAATTPCTT, AAGAATTCTPT, AAGAATTPCTPT, (p is the abbreviation of alpha-putrescinyl residue) were prepared by the phosphotriester method. Nuclease activities and Tm for their oligomers were investigated by comparison of AAGAATTCTT.
D055598:Chemical Phenomena; D002621:Chemistry; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D013941:Thymine / Q000031:analogs & derivatives*
D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; C025113:alpha-putrescinylthymine; D013941:Thymine