Thousand litre scale microcarrier culture of Vero cells for killed polio virus vaccine. Promising results.
Through the progress of scientific knowledge the Vero cell line was considered to be a suitable alternative cell substrate for the industrial production of Polio Virus. Using microcarrier culture, more than 10(12) cells could be obtained weekly for virus inoculation. The virus yield is around 60 D units/ml for type I; 20 D units/ml for type II, and 50 D units/ml for type III.
D000818:Animals; D002460:Cell Line; D008863:Microspheres; D011054:Poliovirus Vaccine, Inactivated / Q000302:isolation & purification*; D014776:Virus Cultivation / Q000379:methods*
D011054:Poliovirus Vaccine, Inactivated