The measurement of monoamine oxidase activity: a comparison of the ion exchange resin column method and the solvent extraction method.
A comparison was made of monoamine oxidase (MAO) activity level; the level was obtained by quantification of radiolabeled deaminated metabolites separated from unchanged amines by two different methods. MAO activity in whole brain of male and female mice from five inbred strains was measured with three substrates PEA, TYR, and 5-HT to examine apparent variations in enzyme activity level with substrate metabolized. Levels of activity measured by the two separation methods were equivalent, as demonstrated by comparison of population mean activity levels and by Pearson product-moment correlation coefficients (0.92, 0.93, and 0.83 for PEA, TYR, and 5-HT, respectively).
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D004796:Clinical Enzyme Tests / Q000379:methods*; D005260:Female; D008099:Liver / Q000201:enzymology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008995:Monoamine Oxidase / Q000032:analysis*
D008995:Monoamine Oxidase