A detailed method for obtaining preparations of human sperm chromosomes.
A detailed technique is described for obtaining preparations of the chromosome complements of human sperm by fertilization of hamster eggs and analysis of the male pronucleus. Some of the more difficult aspects and important steps are emphasized. Technical data from 17 consecutive experiments are presented to provide an estimate of the number of karyotypes which can be obtained in an experiment.
D000818:Animals; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006224:Cricetinae; D004971:Estrus; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D006801:Humans; D007621:Karyotyping / Q000379:methods; D008297:Male; D011247:Pregnancy; D013094:Spermatozoa / Q000648:ultrastructure*; D013480:Superovulation / Q000187:drug effects; D013997:Time Factors