Effect of dazoxiben on platelet-vessel wall interaction.
1 Platelet monolayer adhesion to damaged rabbit aorta and to collagen-coated glass was quantified in a perfusion device. Addition of the thromboxane synthetase inhibitor dazoxiben (UK 37248) 1 and 10 microM reduced adhesion to damaged blood vessel by about 45% at both concentrations, but did not affect adhesion to collagen-coated glass. 2 Measurement by RIA indicated virtual abolition of thromboxane B2 production in the presence of the drug and a slight trend to an increase of 6-oxo-prostaglandin F1 alpha concentration in experiments using aorta. Inhibitory activity of dazoxiben was abolished by previous exposure of the aorta to 15-HPETE(?), an inhibitor of prostacyclin synthetase. 3 The results indicate that dazoxiben inhibits platelet adhesion to vascular subendothelium possibly by promoting synthesis of PGI2 from re-directed platelet endoperoxides.
D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects*; D001808:Blood Vessels / Q000187:drug effects*; D007093:Imidazoles / Q000494:pharmacology*; D066298:In Vitro Techniques; D015289:Leukotrienes; D008054:Lipid Peroxides; D010088:Oxidoreductases / Q000037:antagonists & inhibitors*; D011817:Rabbits; D013930:Thromboxane-A Synthase / Q000037:antagonists & inhibitors*
D001095:Arachidonic Acids; D007093:Imidazoles; D015289:Leukotrienes; D008054:Lipid Peroxides; C029166:dazoxiben; C025086:15-hydroperoxy-5,8,11,13-eicosatetraenoic acid; D010088:Oxidoreductases; D013930:Thromboxane-A Synthase