Direct determination of phenylalanine in serum extracts of phenylketonuria patients by reversed-phase high-performance liquid chromatography.
A method for direct determination of phenylalanine (Phe) in serum extract by reversed-phase high-performance liquid chromatography with an octadecylsilane column has been devised. Phe is monitored at 220 nm with a UV detector. The mobile phase is a mixture of methanol and water (10:90) with added phosphoric acid and potassium dihydrogen phosphate and has a pH value of 4.3. The minimum detectable amount is 6 ng. The average contents of Phe found for healthy adults and children are 12 and 15 micrograms/ml respectively. Phe contents of sera of three phenylketonuria children are about 10 to 15 times higher than these values.
D000328:Adult; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D010649:Phenylalanine / Q000097:blood*; D010661:Phenylketonurias / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet
D010649:Phenylalanine