Assessing a quality control program for erythrocyte preservation and attempts to improve quality of cells after storage at +4 degree C.
About 22 in vitro parameter values were used to evaluate the condition of erythrocytes stored at +4 degrees C in SAGM (1) medium for 28 and 42 d. The parameters were related to such different functions of the erythrocyte, as glycolysis, ion transport, purine metabolism, membrane function and membrane morphology immediately after storage and after subsequent incubation at physiological conditions for 30 min. The evaluation included comparison with posttransfusion viability after 42 d of storage. The investigation gives information in statistical terms about relations between the parameter values and their change with storage time. Total adenylates are superior to ATP in predicting post transfusion viability. There is a good correlation between the in vitro parameters: total adenylates, morphology score and erythrocyte fluidity.
D000227:Adenine Nucleotides / Q000097:blood; D001793:Blood Preservation / Q000592:standards*; D003080:Cold Temperature; D004912:Erythrocytes / Q000502:physiology*; D006801:Humans; D007291:Inosine Monophosphate / Q000097:blood; D011786:Quality Control; D013997:Time Factors
D000227:Adenine Nucleotides; D007291:Inosine Monophosphate