Characterization of an immune suppressor from transformed human trophoblastic JEG-3 cells.
Cultured human choriocarcinoma JEG-3 cells secrete an immunosuppressor that inhibits lymphocyte proliferation stimulated by either an antigen or a mitogen. In this study, the immunosuppressive factor was characterized by three methods: ion-exchange and exclusion chromatography, partition in organic solvents, and thin-layer chromatography on silicic acid. This JEG-3 cell factor appeared to be a protein complex of about 150,000-200,000 Da that contained an immunologically active polar lipid. The structural and functional characteristics of JEG-3 cell immunosuppressor are similar if not identical to those of SIF, a suppressor lymphokine derived from T cells. These secretions from transformed trophoblastic cells may correspond to normal placental products or represent a function of malignant cells.
D002460:Cell Line; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000302:isolation & purification / Q000502:physiology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000302:isolation & purification / Q000502:physiology*; D013491:Suppressor Factors, Immunologic; D013745:Tetanus Toxoid / Q000494:pharmacology; D014327:Trophoblasts / Q000276:immunology*
D008222:Lymphokines; D010835:Phytohemagglutinins; D011257:Pregnancy Proteins; D013491:Suppressor Factors, Immunologic; D013745:Tetanus Toxoid