Growth and oxygen requirements of antibody producing mouse hybridoma cells in suspension culture.
Mouse hybridoma cells producing a monoclonal antibody to anti-B blood group antigen have been grown in airlift fermenters up to 30 litres capacity. As a prerequisite for scaling the process up, the oxygen utilization rate of the cells has been established by a dynamic method using an oxygen electrode. The data is compared with that obtained from a chemostat under oxygen limited steady state conditions.
D000818:Animals; D000921:Antibody-Producing Cells / Q000166:cytology / Q000378:metabolism*; D002455:Cell Division; D002460:Cell Line; D006825:Hybridomas / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D010101:Oxygen Consumption