Concomitant changes of membrane leak permeability and phospholipid dynamics in erythrocytes subjected to chemical and physical membrane perturbation.
A mild selective oxidant of erythrocyte membrane SH-groups producing SS-bonds and inducing reversible crosslinking of spectrin, as well as strong less specific oxidants which produce a more extensive modification of membrane proteins and peroxidation of lipids and dielectric breakdown of the membrane induce formation of structural defects acting as aqueous pores and reorientation sites for phospholipids. The coupling of a process requiring hydrophilic structures (leak permeability) and a process also involving hydrophobic constituents (flip of phospholipids) suggests that the membrane lipid domain is involved in the effects.
D002250:Carbon Radioisotopes; D002463:Cell Membrane Permeability; D004910:Erythrocyte Membrane / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D008244:Lysophosphatidylcholines / Q000097:blood; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000097:blood*; D010743:Phospholipids / Q000097:blood*
D002250:Carbon Radioisotopes; D008244:Lysophosphatidylcholines; D008563:Membrane Lipids; D010743:Phospholipids