Transformation of Hprt gene with sperm DNA.
Inactivation of the X chromosome during mammalian spermatogenesis has been postulated to occur by the same mechanism that controls female somatic X chromosome inactivation. We have used DNA-mediated transformation of HPRT- cells to test this idea, because it has been shown previously that inactive X chromosome DNA from somatic cells will not transform HPRT- cells. Isolated DNA from the mature sperm of five mammals (human, mouse, horse, bull, rabbit) were all capable of HPRT transformation, and transformants were confirmed electrophoretically. Measures were taken to ensure that the transformation frequencies observed could not be due to somatic contamination. The positive HPRT transformation result indicates that mature sperm X chromosomal DNA is not modified in the same manner as that of female inactive X chromosomal DNA. Since there is evidence for methylation of the somatic inactive X chromosome, it is possible that methylation, at least for the genes studied, is not involved in sperm X chromosome inactivation.
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D004247:DNA / Q000235:genetics*; D005260:Female; D005796:Genes; D006736:Horses; D006801:Humans; D008297:Male; D051379:Mice; D011817:Rabbits; D013091:Spermatogenesis; D013094:Spermatozoa / Q000648:ultrastructure*; D014170:Transformation, Genetic; D014960:X Chromosome / Q000648:ultrastructure
D004247:DNA