title: Localization of carnitine acyltransferases and acyl-CoA beta-oxidation enzymes in small intestinal microperoxisomes (peroxisomes) of normal and clofibrate treated mice.
abstract: Dietary clofibrate for 21 days induced a rise in the specific activities of crotonase, acyl-CoA oxidase and carnitine acetyltransferase in a crude particulate fraction from mouse small intestinal mucosa. Subcellular fractionation of post-nuclear supernatant prepared from mucosal homogenates of normal and clofibrate treated animals allowed substantial separation of peroxisomes from contaminating organelles. Analysis of fractions demonstrated that intestinal peroxisomes contain acyl-CoA oxidase, crotonase, beta-hydroxyacyl-CoA dehydrogenase and carnitine acyltransferase activities. It is concluded that intestinal peroxisomes are equipped to engage in fatty acid oxidation.
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism*; D042962:Acyl-CoA Oxidase; D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D002333:Carnitine Acyltransferases / Q000378:metabolism*; D002332:Carnitine O-Acetyltransferase / Q000378:metabolism; D002994:Clofibrate / Q000494:pharmacology*; D004746:Enoyl-CoA Hydratase / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects / Q000201:enzymology*; D007421:Intestine, Small / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D051379:Mice; D008830:Microbodies / Q000187:drug effects / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism
chemical_list: D000214:Acyl Coenzyme A; D010088:Oxidoreductases; D042962:Acyl-CoA Oxidase; D000217:Acyltransferases; D002333:Carnitine Acyltransferases; D002332:Carnitine O-Acetyltransferase; D004746:Enoyl-CoA Hydratase; D002994:Clofibrate