[Properties of a bacterial enzyme preparation of alkylsulfatase].
A preparation of primary alkyl sulfatase was obtained from the culture of Pseudomonas species 2T/1. It can hydrolyze alkyl sulfates, which belong to anion surface-active compounds, to sulfate ion and fatty alcohol, and as a result the harmful for biosphere property of the surface activity is gone. pH and temperature of the incubation mixture, the presence of ions of some bivalent metals and components of synthetic detergents (SD), composition of the buffer mixture and substrate concentration affect the rate of sodium dodecylsulfate (SDS) hydrolysis. The alkyl sulfatase preparation is relatively stable. The maximum rate of SDS hydrolysis was found to be at 70 degrees C. The preparation catalyzes the hydrolysis of some alkyl sulfate homologues and industrial alkyl sulfates. The temperature optimum of the preparation is 40 degrees C, the pH-optimum is 8.0-9.0.
D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D011549:Pseudomonas / Q000201:enzymology*; D012722:Sewage; D013379:Substrate Specificity; D013429:Sulfatases / Q000302:isolation & purification* / Q000378:metabolism; D013463:Sulfuric Acid Esters / Q000378:metabolism; D013501:Surface-Active Agents / Q000378:metabolism; D013696:Temperature
D012722:Sewage; D013463:Sulfuric Acid Esters; D013501:Surface-Active Agents; D013429:Sulfatases; C019244:alkylsulfatase
Svoĭstva bakterial'nogo fermentnogo preparata alkilsul'fatazy.