title: 'Cap 90', a 90-kDa Ca2+-dependent F-actin-capping protein from vertebrate brain.
abstract: A Ca2+-dependent actin filament-capping protein of 90 kDa was purified from bovine brain using a new and rapid isolation procedure. This basically includes affinity purification on DNase-I agarose. The protein caps the fast-growing end of actin filaments but has no fragmenting or severing activity. Using Triton X-100-extracted cytoskeletons, capping and severing activities of actin-binding proteins become clearly distinguishable from each other.
mesh_terms: D051339:Actin Depolymerizing Factors; D000199:Actins / Q000378:metabolism*; D000818:Animals; D001923:Brain Chemistry; D002118:Calcium / Q000502:physiology; D002417:Cattle; D003599:Cytoskeleton / Q000378:metabolism; D051345:Destrin; D008840:Microfilament Proteins; D008854:Microscopy, Electron; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D051339:Actin Depolymerizing Factors; D000199:Actins; D051345:Destrin; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D002118:Calcium