The sensitization potential of D & C Yellow No. 11 in guinea pigs.
The certified drug and cosmetic dye substance known as D & C Yellow No. 11 [2-(2-quinolyl)-1,3 indandione] was tested for its dermal sensitization potential in guinea pigs. An induction regimen consisting of a single footpad injection of Complete Freund's Adjuvant diluted with antigen, and an intradermal injection of antigen, produced delayed-type hypersensitivity reactions. A high sensitization frequency was observed with 50 micrograms D & C Yellow No. 11 and the positive control 2,4-dinitrochlorobenzene (DNCB). The reaction severities of both DNCB and 50 micrograms D & C Yellow No. 11 were comparable. A dose-response profile was obtained with D & C Yellow No. 11 employing several concentrations (50, 250 and 500 micrograms/ml). Histologic examination of selected skin sites demonstrated a cellular inflammatory response which was consistent with delayed-type hypersensitivity.
D000818:Animals; D004396:Coloring Agents / Q000276:immunology*; D004137:Dinitrochlorobenzene; D004305:Dose-Response Relationship, Drug; D004342:Drug Hypersensitivity / Q000175:diagnosis / Q000276:immunology*; D005260:Female; D005620:Freund's Adjuvant; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000139:chemically induced* / Q000175:diagnosis / Q000276:immunology; D007114:Immunization / Q000379:methods*; D007271:Injections, Intradermal / Q000379:methods; D011804:Quinolines / Q000008:administration & dosage / Q000276:immunology*; D012867:Skin / Q000276:immunology*
D004396:Coloring Agents; D004137:Dinitrochlorobenzene; D011804:Quinolines; C027012:2-((2'-quinolinyl)-1,3-indandione); D005620:Freund's Adjuvant