Do cells of continually renewing populations and those stimulated from quiescence respond similarly to HU and Ara-C?
The cytocidal and cytostatic effects of a single dose of either hydroxyurea (HU) or cytosine arabinoside (Ara-C) have been studied in a variety of tissues, from both rats and mice, by autoradiographic and histological methods. The necrogenic effects of the drugs on DNA-synthesising cells from continually renewing populations have been compared with the effects on cells induced to enter DNA synthesis from a formerly quiescent state. Four models of stimulated proliferation have been studied; the isoprenaline-stimulated salivary glands, the testosterone-stimulated seminal vesicle in the castrate, the regenerating liver after two-thirds partial hepatectomy, and a model of diethylnitrosamine (DEN)-induced hepatocyte proliferation. The time course of the cytokinetic response in each tissue was followed for a period of 24 h after drug treatment. The cytostatic effects of the drugs were similar in all tissues investigated, DNA synthesis being inhibited for a 4- to 5-h period. However, the sensitivity to the lethal effects showed both tissue and species variability.
D000818:Animals; D001345:Autoradiography; D002470:Cell Survival / Q000187:drug effects; D003561:Cytarabine / Q000633:toxicity*; D004247:DNA / Q000096:biosynthesis*; D006918:Hydroxyurea / Q000633:toxicity*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D014316:Tritium
D003561:Cytarabine; D014316:Tritium; D004247:DNA; D006918:Hydroxyurea