Development of a Babesia rodhaini mouse assay for quality control of the serum component of a bovine babesiosis vaccine.
The rodent babesia, Babesia rodhaini, survived equally well in basal medium containing either 10 per cent rat serum or 10 per cent bovine serum. As a result, a B rodhaini mouse assay is now performed routinely to determine the suitability of batches of bovine serum for use in a commercial Babesia bovis vaccine issued in Australia. Heat inactivation of bovine serum at 56 degrees C for two hours did not affect the survival of either B bovis or B rodhaini.
D000818:Animals; D001403:Babesia / Q000502:physiology; D001404:Babesiosis / Q000517:prevention & control*; D001681:Biological Assay / Q000379:methods; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D002470:Cell Survival; D051379:Mice; D008808:Mice, Inbred CBA; D010949:Plasma; D014612:Vaccines / Q000592:standards*
D014612:Vaccines