Neuronal cultures on microcarriers: dissociated spinal cord cells.
Dissociated spinal cord cells from rat fetuses were successfully cultured up to the state of synaptogenesis and myelin formation on DEAE cellulose cylindrical microcarriers. Nerve cells and microcarriers interconnected to form conglomerates offering optimal nutritional conditions which enhanced cell maturation. These conglomerates yielded large amounts of cells which could easily be harvested for biochemical analysis.
D000818:Animals; D002478:Cells, Cultured; D005333:Fetus / Q000166:cytology; D008863:Microspheres; D009186:Myelin Sheath / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology*; D051381:Rats; D013116:Spinal Cord / Q000166:cytology*; D013569:Synapses / Q000648:ultrastructure