Determination of the specific activity of NaB3H4.
A method for the rapid determination of the specific activity of NaB3H4 is presented. NaB3H4 is used to reduce NAD+ to [3H]NADH, which is then isolated by anion exchange chromatography. The specific activity of the NaB3H4 is calculated from measurements of radioactivity and absorbance (340 nm) in the [3H]NADH fractions.
D001894:Borohydrides / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D009243:NAD / Q000302:isolation & purification; D012588:Scintillation Counting; D013056:Spectrophotometry, Ultraviolet; D014316:Tritium / Q000032:analysis
D001894:Borohydrides; D009243:NAD; D014316:Tritium; C025364:sodium borohydride