Preparation of native connectin from chicken breast muscle.
Connectin, an elastic protein of striated muscle, was isolated in a native state from chicken breast muscle. Myofibrils were well washed with 5 mM NaHCO3 and extracted with 0.1 M sodium phosphate buffer, pH 5.6. After washing with water, myofibrils were extracted with 0.1 M phosphate buffer, pH 6.6. The filtrate contained native connectin. The yield of connectin was as high as 200 mg, starting from 50 g of muscle. Since connectin had been isolated only in a denatured state before, the new preparation method enables investigation of possible interactions of connectin with other myofibrillar structural proteins.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002645:Chickens; D064211:Connectin; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009124:Muscle Proteins / Q000302:isolation & purification*; D009132:Muscles / Q000032:analysis*; D009210:Myofibrils / Q000032:analysis*; D011494:Protein Kinases; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D064211:Connectin; D009124:Muscle Proteins; D011494:Protein Kinases