Inhibition of forskolin-stimulated cardiac adenylate cyclase activity by short-chain alcohols.
The diterpene forskolin stimulated rat cardiac adenylate cyclase activity at least 20-fold and potentiated the effect of NaF. The stimulatory effect of forskolin was reduced in the presence of Gpp(NH)p. Ethanol markedly reduced the stimulation of adenylate cyclase by forskolin while potentiating NaF and Gpp(NH)p stimulation. The inhibitory effect of ethanol on forskolin stimulation appeared to be of a mixed type with both a competitive and a non-competitive component. Three other short-chain linear alcohols (methanol, propanol, butanol) also inhibited forskolin-stimulation, this effect being proportional to the number of carbon atoms.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000438:Alcohols / Q000494:pharmacology*; D000818:Animals; D005576:Colforsin; D004224:Diterpenes / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D051381:Rats; D012969:Sodium Fluoride / Q000494:pharmacology
D000438:Alcohols; D004224:Diterpenes; D005576:Colforsin; D006165:Guanylyl Imidodiphosphate; D000431:Ethanol; D012969:Sodium Fluoride; D000262:Adenylyl Cyclases