Horse lymphocyte cell synchronization: improved technique for chromosome analysis.
A method using methotrexate for horse lymphocyte cell synchronization and thymidine for incorporation into DNA replication is described. This method provides a powerful technique for the study of chromosomal abnormalities and detailed analysis of chromosomal replication patterns. The determination of horse karyotypes with many similar chromosomes needs a special method which reveals the numerous and informative stages of the cell cycle. Horse lymphocyte cell cultures treated with colcemid (20 min) and harvested 6 hours after the release of the 17 hour-block with methotrexate show the best results for exact identification and analysis of horse chromosomes.
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D002869:Chromosome Aberrations; D002871:Chromosome Banding / Q000662:veterinary; D004261:DNA Replication / Q000187:drug effects; D006736:Horses / Q000235:genetics*; D007621:Karyotyping / Q000662:veterinary; D008214:Lymphocytes / Q000648:ultrastructure*; D008727:Methotrexate / Q000494:pharmacology*
D008727:Methotrexate