Effect of various proteinase inhibitors on ovulation of explanted hamster ovaries.
Hamster ovaries explanted at 21:00-24:00 h on the day of pro-oestrus were incubated with microbial proteinase inhibitors until 10:30 h on the next morning and the ovulatory blocking effect of these inhibitors was examined. Amongst 11 proteinase inhibitors examined, talopeptin, a specific inhibitor for metallo-proteinases, and alpha-MAPI, a specific inhibitor for serine and thiol proteinases, were the strongest blockers. These 2 inhibitors exhibited a chronological discrepancy in their blocking effect on ovulation. S-SI, plasminostreptin, elastatinal, antipain and chymostatin, which are inhibitors for serine proteinases, partly but significantly inhibited ovulation. The results suggest that, in addition to a metallo-proteinase reported previously, a proteinase which is sensitive to alpha-MAPI is essential for the ovulatory process, and that serine proteinase(s) also participate in ovulation of the hamster ovary.
D000818:Animals; D000900:Anti-Bacterial Agents; D006224:Cricetinae; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006020:Glycopeptides / Q000494:pharmacology; D009924:Organ Culture Techniques; D010053:Ovary / Q000187:drug effects*; D010060:Ovulation / Q000187:drug effects*; D010455:Peptides; D011247:Pregnancy; D011359:Proestrus; D011480:Protease Inhibitors / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D006020:Glycopeptides; D010455:Peptides; D011480:Protease Inhibitors; C038356:microbial alkaline proteinase inhibitor; C037819:talopeptin