A new, convenient method for determining T residues in chemical DNA sequencing by using photoreaction with spermine.
Irradiation of 3'-[32p]-labeled DNA fragments with 254-nm light in the presence of spermine resulted in a T specific cleavage of the DNA chain without piperidine treatment. In the presence of methylamine G + T reactions (G greater than T) were observed under the same irradiation conditions. The T reaction with spermine and the G greater than reaction with methylamine may be combined with the standard Maxam-Gilbert's C + T reaction to provide confirmatory determination of DNA sequences.
D001483:Base Sequence; D004247:DNA; D008967:Molecular Biology / Q000379:methods; D013096:Spermine; D013942:Thymine Nucleotides
D013942:Thymine Nucleotides; D013096:Spermine; D004247:DNA