Radioimmunoassay of delta sleep-inducing peptide using an iodinated p-hydroxyphenylpropionic acid derivative as tracer.
A highly sensitive radioimmunoassay for delta sleep-inducing peptide (DSIP) has been developed. A p-hydroxyphenylpropionic acid conjugate of DSIP was used for radioiodination. Using reversed-phase high performance liquid chromatography the labelled DSIP derivative was isolated in a high yield and with a high specific activity. The assay allows measurement of DSIP-like material in body fluids with a minimum detectable concentration of 0.1 ng/ml standard DSIP (10 pg/tube).
D003701:Delta Sleep-Inducing Peptide; D006801:Humans; D007106:Immune Sera; D007457:Iodine Radioisotopes; D008832:Microchemistry; D009842:Oligopeptides / Q000097:blood* / Q000134:cerebrospinal fluid / Q000652:urine; D010666:Phenylpropionates; D011863:Radioimmunoassay / Q000379:methods
D007106:Immune Sera; D007457:Iodine Radioisotopes; D009842:Oligopeptides; D010666:Phenylpropionates; D003701:Delta Sleep-Inducing Peptide; C008869:phloretic acid