An affinity ligand for phospholipase A2 purification.
An alkyl ether analog of phosphatidylcholine was synthesized and used as a ligand to purify acid-extracted phospholipase A2 from bovine ileum smooth muscle by affinity chromatography in the presence of cholate. This ligand contains a primary amino group at the omega-position of the acyl chain in position 1 and so permits direct covalent coupling with the ester group of Affi-Gel-10. An endogenous membrane bound phospholipase A2 has been purified 32-fold in a good yield (70%) employing this ligand in an affinity chromatography step.
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D007082:Ileum / Q000201:enzymology; D008024:Ligands / Q000138:chemical synthesis; D010740:Phospholipases / Q000302:isolation & purification*; D010741:Phospholipases A / Q000302:isolation & purification*; D054467:Phospholipases A2
D008024:Ligands; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2