Characterization and preliminary mapping of cauliflower mosaic virus transcripts.
Using Northern blot analysis we have studied the transcription of the CM4-184 Ga. strain of cauliflower mosaic virus (CaMV) DNA. This analysis reveals that this CaMV strain, like the Cabb-BS and the Cabb B-JI strains, produces both a genomic length transcript and a 1900-nucleotide (nt) transcript during infection. In addition, we detect an 1800-nt PA+ transcript mapping primarily to the EcoRI-c region of the virus, and three apparent minor viral-specific PA + RNAs of 4900, 4500, and 4300 nt. We also report the presence of two small viral single-stranded DNAs produced during infection, and show that both DNAs are derived from the EcoRI-b region of the virus.
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D009029:Mosaic Viruses / Q000235:genetics*; D010944:Plants / Q000382:microbiology; D012367:RNA, Viral / Q000096:biosynthesis; D014158:Transcription, Genetic
D004279:DNA, Viral; D012367:RNA, Viral