Exogenous methionine depresses level of mRNA for a soybean storage protein.
In vitro translation experiments indicate that absence of the beta-subunit of 7S storage protein in soybean (Glycine max L. Merr. cv. Provar) cotyledons cultured on methionine-supplemented medium is due to lack of functional mRNA for that polypeptide. Relative amounts of functional mRNA for the 7S alpha'- and alpha-subunits were unaffected by methionine in the cotyledon culture medium. Measurements of beta-subunit accumulation in cotyledons transferred from basal medium to methionine-supplemented medium show that methionine inhibits continued accumulation of the beta-subunit after synthesis of the beta-subunit has begun, and that methionine does not promote degradation of existing beta-subunit.
D052179:Antigens, Plant; D005916:Globulins / Q000235:genetics*; D008715:Methionine / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics*; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism*; D055314:Seed Storage Proteins; D030262:Soybean Proteins; D013025:Glycine max
D052179:Antigens, Plant; D005916:Globulins; D010940:Plant Proteins; D012333:RNA, Messenger; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; D008715:Methionine