Intracellular catabolin-like activity in cultured synovial tissue.
The time course of the synthesis and secretion of catabolin-like activity by porcine synovial tissue was determined. Ex vivo tissue had no demonstrable activity, but short-term culture (less than 1 h) induced cellular accumulation of activity. This accumulation, which preceded secretion, was reversibly inhibited by cycloheximide. The activity was localized in the cystol fraction.
D000818:Animals; D002451:Cell Compartmentation; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D007375:Interleukin-1; D053583:Interleukin-1beta; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D013552:Swine; D013583:Synovial Membrane / Q000378:metabolism*
D007375:Interleukin-1; D053583:Interleukin-1beta; D011506:Proteins; D003513:Cycloheximide