Incorporation of radioactive precursors into glycosaminoglycans by rat muscle fibroblasts exposed to a solubilized rat bone matrix fraction.
Confluent cultures of rat muscle fibroblastic cells respond by increased glycosaminoglycan (GAG) synthesis when cultured in medium containing a solubilized bone matrix fraction (SBM) at a concentration of 100 micrograms/ml. The metabolism of the GAG associated with the cell pellet, the cell surface and the tissue culture medium fractions was studied, in the presence and absence of SBM, by measuring the incorporation of radioactivity from [3H]glucosamine and [35S]SO4 into the isolated GAG. Net synthesis of hyaluronic acid and of chondroitin sulfate in the medium fraction increased more rapidly in cultures containing SBM compared to controls, and the accumulation of labelled GAG in the medium of the treated cultures was approximately linear with respect to the length of incubation. The addition of SBM also resulted in increased incorporation of 3H and of 35S into the GAG of the cell surface and cell pellet fractions. In these fractions, stimulation of incorporation of radioactivity occurred in two waves: an early, relatively minor increase and a later relatively major increase. The relatively major stimulation of radioactivity into the GAG of the cell surface fraction occurred between 24 and 48 h and was independent of any apparent effect of serum.
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D001857:Bone Matrix / Q000502:physiology*; D002478:Cells, Cultured; D005591:Chemical Fractionation; D005347:Fibroblasts / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism*; D009132:Muscles / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D013431:Sulfates / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D014316:Tritium
D006025:Glycosaminoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes; D014316:Tritium; D005944:Glucosamine