title: Identification and visualization of the sexual agglutinin from the mating-type plus flagellar membrane of Chlamydomonas.
abstract: Sexual agglutinins located on the flagellar membranes of Chlamydomonas gametes mediate a mating-type-specific adhesion reaction that brings complementary gametes together for zygotic cell fusion. We identify the mating-type plus agglutinin, using a combination of biochemical and genetic analysis, as a glycopolypeptide with an apparent molecular weight of greater than 10(6) by SDS-polyacrylamide gel electrophoresis. Its core polypeptide migrates as a approximately 480-kd species, and it is estimated to be present in approximately 30 copies per gametic flagellum. The agglutinin is present in the wild type, in a mutant that agglutinates but cannot fuse, and in a complementing diploid, whereas it is absent from four nonagglutinating mutants and from a noncomplementing diploid. Electron microscopy shows the purified agglutinin to be a highly asymmetric molecule, 220 X 4 nm. To our knowledge, this is the first reported purification and visualization of a membrane-associated cell-cell recognition protein.
mesh_terms: D000373:Agglutinins / Q000302:isolation & purification*; D002448:Cell Adhesion; D002462:Cell Membrane / Q000032:analysis; D002696:Chlamydomonas / Q000032:analysis* / Q000235:genetics / Q000648:ultrastructure; D005407:Flagella / Q000032:analysis*; D006023:Glycoproteins / Q000302:isolation & purification; D008970:Molecular Weight; D012098:Reproduction
chemical_list: D000373:Agglutinins; D006023:Glycoproteins