Human interferons and their purification.
The present paper describes briefly the procedure used in our laboratory for the preparation of HuIFN-alpha. Induction of interferon was carried out in the suspension of human leukocytes from peripheral blood, using Newcastle virus as inducer. The optimal conditions to produce interferon were established in the media with and without serum. Moreover, the work reports the results of studies on the purification of HuIFN-alpha and HuIFN-beta interferons.
D002478:Cells, Cultured; D006801:Humans; D007369:Interferon Inducers; D007370:Interferon Type I / Q000096:biosynthesis / Q000302:isolation & purification*; D007962:Leukocytes / Q000276:immunology
D007369:Interferon Inducers; D007370:Interferon Type I