Biosynthetic Met-hGH: lack of immunoreactivity for other pituitary hormones.
The immunoreactivities of 5 hGH preparations were examined in RIA systems for hPRL, hACTH, hLH, hFSH and hTSH. All preparations derived from pituitary extracts showed distinct displacements in almost all RIA systems when added 10(3) to 10(4) times larger amounts than the proper hormones. On the other hand, biosynthetic methionyl-hGH did not manifest any immunoreactivity in any system with comparable amounts. These results indicate that the displacements caused by hGH preparations in RIA systems for other pituitary hormones are solely due to contamination, and not due to intrinsic cross reaction. In in vitro and in vivo studies using extractive preparations of hGH one should be cautious about contamination of other pituitary hormones.
D013006:Growth Hormone / Q000031:analogs & derivatives* / Q000276:immunology; D019382:Human Growth Hormone; D006801:Humans; D010907:Pituitary Hormones / Q000276:immunology*; D011863:Radioimmunoassay
D010907:Pituitary Hormones; D019382:Human Growth Hormone; D013006:Growth Hormone