Efficient transfection of mammalian cells with viral DNA in optimal culture conditions.
Standard or newly developed DNA transfection procedures were compared for both innocuity and efficiency using simian virus 40 (SV40) DNA and monkey kidney (Vero) cells as an indicator system. Optimal results have been obtained by treating the delicate cell monolayers with a solution of glycerol before infection with DNA-calcium phosphate complexes.
D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D002460:Cell Line; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000235:genetics*; D003851:Deoxyribonucleases / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D007668:Kidney; D008722:Methods; D014162:Transfection / Q000187:drug effects
D004279:DNA, Viral; D003851:Deoxyribonucleases; D002122:Calcium Chloride; D005990:Glycerol