[Desthiobiotin transformation to biotin by the fungus Rhizopus delemar].
The object of this work was to study the transformation of desthiobiotin (DTB), a biotin precursor, to biotin by Rhizopus delemar which can accumulate rather large quantities of biotin in the medium. Both the growing and resting culture transform DTB at a high rate. Biotin excretion by the resting culture rises by 100% when 25 micrograms/ml of DTB are added to the medium. The content of biotin within the cells remains unchanged. L-Methionine, sodium sulfite and sodium sulfate are most effective sulfur donors for DTB transformation by the resting culture. Cystine, cysteine and elementary sulfur cannot serve as sulfur donors. The transforming activity of the mycelium is preserved for about seven days. Immobilization of the cells in agar gel and in porolon does not increase the time within which the mycelium remains in the active state.
D001710:Biotin / Q000031:analogs & derivatives* / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D001711:Biotransformation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D012233:Rhizopus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D013455:Sulfur / Q000494:pharmacology
D001710:Biotin; D013455:Sulfur; C004749:desthiobiotin
Transformatsiia destiobiotina v biotin gribom Rhizopus delemar.