Enhancement of human myeloid stem cell growth in vitro.
Human myeloid stem cell growth in agar was enhanced by the addition of human plasma, red-cell lysate, hemin, and beta-mercaptoethanol to the culture system. Using conditioned media from a human T-cell or leukocyte-conditioned media as a source of colony-stimulating activity, the number of colonies was increased due to enhancement by an average of 54%. Analysis of colony types indicated that over 80% of the increase in colony number at eight days was due to granulocytic colony growth. Light density marrow cells cultured with enhancing agents produced colony levels of approximately 12-27 CFU-C/2 x 10(3) cells.
D002999:Clone Cells; D003470:Culture Media; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000502:physiology*; D006427:Hemin / Q000494:pharmacology; D006801:Humans; D007962:Leukocytes; D008623:Mercaptoethanol / Q000494:pharmacology; D010949:Plasma
D003470:Culture Media; D008623:Mercaptoethanol; D006427:Hemin