A comparison of protein synthetic activity in in vitro cultured denuded and follicle-enclosed oocytes.
We have studied in vitro protein synthesis in mouse denuded and follicle-enclosed oocytes by measuring 3H-leucine incorporation into TCA-precipitable material. Thirty percent more radiolabelled TCA-precipitable material was found in follicle-enclosed oocytes. Since in our experimental conditions the internal specific activity of 3H-leucine does not differ in the two systems, and no appreciable transfer of labelled protein from follicle cells to the oocyte occurs, we conclude that follicle cells have a beneficial influence on the oocyte's protein synthetic activity.
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism; D051379:Mice; D009865:Oocytes / Q000378:metabolism*; D006080:Ovarian Follicle / Q000166:cytology; D014176:Protein Biosynthesis
D007930:Leucine