Assignment of the native Chinese hamster dihydrofolate reductase gene to chromosome 2.
The native Chinese hamster DHFR gene was localized to 2pter----q14 using a combination of somatic cell hybrid technology and molecular techniques. In addition, PGD, ENO1, and DTS, previously mapped to Chinese hamster chromosome 2, were localized to 2q14----qter. Localization of PGM1 to this region was confirmed. The relationship of the site of the native DHFR gene to the location of the homogeneously staining region in methotrexate-resistant cells is discussed.
D000818:Animals; D002874:Chromosome Mapping; D006224:Cricetinae / Q000235:genetics*; D003412:Cricetulus / Q000235:genetics*; D004351:Drug Resistance; D008040:Genetic Linkage; D006822:Hybrid Cells; D007621:Karyotyping; D008727:Methotrexate / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate