A new muscle phenotype is expressed by subcultured quail myoblasts isolated from future fast and slow muscles.
Differentiation of quail myoblasts, isolated from thigh pectoralis and anterior latissimus dorsi muscle, was analyzed in primary cultures and in cultures obtained following repeated subculturing. Our study shows that quail myoblasts can survive many generations without losing their ability to form myotubes. However, during these subcultures the cells progressively express a new phenotype. This phenotype is characterized by a mixture of myosin light chains such that LC1F, LC2F, and LC2S are present in roughly equimolar amounts, each accounting for 25 to 30% of the total light chain synthesis while LC1S accounts for the remaining 10 to 15%, and by a mixture of fast and slow alpha tropomyosin in which alpha S accounts for 10 to 15% of the alpha subunits synthesis. Clonal analysis indicates that all cells in the population express this phenotype which is also characteristic of subcultures obtained from both future fast and slow muscles. Relationships between this phenotype and muscle development are discussed.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000096:biosynthesis; D007700:Kinetics; D009124:Muscle Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D009132:Muscles / Q000502:physiology*; D009219:Myosin-Light-Chain Kinase; D010641:Phenotype; D011494:Protein Kinases / Q000096:biosynthesis; D011784:Quail
D007527:Isoenzymes; D009124:Muscle Proteins; D011494:Protein Kinases; D009219:Myosin-Light-Chain Kinase