title: Relationship between adenylate cytokinin production and Ti plasmid of Agrobacterium tumefaciens.
abstract: To know whether the tumor-inducing plasmid of Agrobacterium tumefaciens carries genetic information of the biosynthesis of cytokinins, the levels of 6-(3-methyl-2-butenyl-amino)purine (iPAde) and its 4-hydroxy derivative trans-zeatin (trans-Z) and its p-beta-D-ribofuranoside (trans-ZR) produced in media by wild-type virulent strain, plasmid-cured avirulent strain and the deletion mutant were compared. The highest levels of iPAde and trans-Z were found in the culture filtrate of late-log phase growth of plasmid-containing virulent strain, then the levels of iPAde and trans-Z were reduced rapidly at stationary phase. The plasmid-cured avirulent strain and deletion mutant had low levels of iPAde and trans-Z throughout the growth. Results obtained here showed Ti plasmid plays an important role in cytokinin biosynthesis.
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000096:biosynthesis; D000241:Adenosine / Q000031:analogs & derivatives / Q000096:biosynthesis; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D003583:Cytokinins / Q000096:biosynthesis*; D007541:Isopentenyladenosine / Q000031:analogs & derivatives / Q000096:biosynthesis; D010937:Plant Growth Regulators / Q000096:biosynthesis*; D010957:Plasmids; D012231:Rhizobium / Q000254:growth & development / Q000378:metabolism*; D015026:Zeatin / Q000096:biosynthesis
chemical_list: D003583:Cytokinins; D010937:Plant Growth Regulators; C001478:N(6)-(delta(2)-isopentenyl)adenine; D007541:Isopentenyladenosine; D015026:Zeatin; C009699:zeatin riboside; D000225:Adenine; D000241:Adenosine