Separation of human colostral macrophages and neutrophils on gelatin and collagen-serum substrata.
A new technique for separation of human colostral macrophages and neutrophils was developed. Macrophages attached more readily to acid soluble collagen-serum or gelatin-serum substrata than neutrophils. Most of the adherent neutrophils were removed during the first 5 minutes incubation in 3 mM EDTA, thereafter, complete detachment of macrophages followed. Neutrophils and macrophages were enriched more than 80% in nonadherent cell populations and detached cell populations, respectively. These separated colostral leukocytes retained their viability and phagocytic activities. Therefore, functional studies of these purified human colostral leukocytes are possible.
D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods*; D003094:Collagen; D003126:Colostrum / Q000166:cytology*; D003470:Culture Media; D005260:Female; D005780:Gelatin; D006801:Humans; D008264:Macrophages / Q000166:cytology* / Q000502:physiology; D009504:Neutrophils / Q000166:cytology* / Q000502:physiology; D011247:Pregnancy
D003470:Culture Media; D005780:Gelatin; D003094:Collagen