The role of intestinal microflora in the metabolic reduction of 1-nitropyrene to 1-aminopyrene in conventional and germfree rats and in humans.
1-Nitropyrene (100 mg/kg body wt.) was administered by gavage to conventional and germfree Fischer 344 rats. 1-Aminopyrene was detected in the feces of conventional rats (5-6% of the dose) but not in the feces of germfree rats. Conjugates of 1-aminopyrene were not detected in the feces of germfree rats and neither 1-aminopyrene nor its conjugates were found in the urine of either conventional or germfree rats. Since reduction of 1-nitropyrene to 1-aminopyrene is an activation process, the results indicate that intestinal microflora are important in the metabolic activation of 1-nitropyrene in vivo in the rat. Human intestinal microflora also reduced 1-nitropyrene to 1-aminopyrene.
D000693:Anaerobiosis; D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D001711:Biotransformation; D005243:Feces / Q000032:analysis; D005856:Germ-Free Life; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D008297:Male; D010084:Oxidation-Reduction; D011721:Pyrenes / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344
D011721:Pyrenes; C004482:1-aminopyrene; C032668:1-nitropyrene