L-Isoleucine and L-leucine are promoters of bladder cancer in rats.
We developed a short term assay for screening promoters of bladder cancer. This assay, in which maintenance of concanavalin A-agglutination of isolated rat bladder cells induced by subcarcinogenic treatment with bladder carcinogen is measured, suggested the possible promoting effects of L-isoleucine, L-leucine, D-tryptophan, and L-valin. Long term in vivo carcinogenesis experiments were carried out on L-isoleucine and L-leucine and it was shown that both were, in fact, promoters of bladder cancer in rats.
D000818:Animals; D002085:Butylhydroxybutylnitrosamine; D002273:Carcinogens; D002478:Cells, Cultured; D007532:Isoleucine / Q000633:toxicity*; D007930:Leucine / Q000633:toxicity*; D008297:Male; D051381:Rats; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced*
D002273:Carcinogens; D007532:Isoleucine; D002085:Butylhydroxybutylnitrosamine; D007930:Leucine