Isolation and partial characterization of equine alveolar macrophages.
A device was constructed from an equine nasogastric tube, polyethylene tubing, and a 3-way stopcock and used to lavage the lungs of anesthetized ponies. The technique was safe and atraumatic in that 6.4 to 19.7 X 10(7) purified alveolar macrophages were removed from the lungs without harm to the ponies or contamination of the samples with blood. Studies of these highly purified cell suspensions revealed a mean viability of 85% as assessed by eosin dye exclusion with a mean recovery (+/- SD) of 12.5 +/- 4.8 X 10(7) pulmonary alveolar macrophages/pony.
D000818:Animals; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002469:Cell Separation; D006736:Horses / Q000033:anatomy & histology*; D008264:Macrophages / Q000166:cytology* / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D011650:Pulmonary Alveoli / Q000166:cytology*; D007507:Therapeutic Irrigation / Q000295:instrumentation / Q000662:veterinary
D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase