Effect of ionizing radiation on synthesis of sub-replicon size DNA in ataxia telangiectasia cells.
The effect of gamma-radiation on the synthesis of short fragments of DNA was studied in control and ataxia telangiectasia lymphoblastoid cell lines. Cells were pulsed with [3H]thymidine for 90 sec, which resulted in labelling of DNA fragments ranging from 0.1-2.1 x 10(7) daltons in size in both control and ataxia telangiectasia cells. After exposure to 800 rad of gamma-rays, a decrease in the formation of DNA over this molecular weight range was observed in four control cell lines, 30 min post-irradiation. Four ataxia telangiectasia cell lines however exhibited no reduction in the synthesis of sub-replicon size DNA.
D001260:Ataxia Telangiectasia / Q000378:metabolism*; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication / Q000528:radiation effects*; D005720:Gamma Rays; D006801:Humans; D008970:Molecular Weight; D013936:Thymidine / Q000378:metabolism
D004247:DNA; D013936:Thymidine