Morphologic study on aorta dot stained with Evans blue in normal and hypercholesterolemic rabbits.
When a normal rabbit is injected intravenously with Evans blue, focal blue areas showing strong permeability appear in a region of the aortic arch and in a region distal to the orifice of the aortic branches. These blue areas consist of the dot stained area but are not homogeneous blue area. The morphological changes in the intima of the dot stained area and the relationship between the appearance of dot stained area and lipid deposition in the aorta of cholesterol fed rabbit were investigated. Evans blue showed focal acceleration of the permeability at the region of the aortic arch as early as 10 minutes. Light and electron microscopically such dot stained areas showed the widening of subendothelial space, lifting of some endothelial cells, intracytoplasmic vacuoles and membranous structure, junctional dilatation, and enhanced permeability of peroxidase. In 2% cholesterol fed rabbits, lipid deposition showed a punctuate pattern similar to the dot stained area of normal rabbit.
D000818:Animals; D001011:Aorta / Q000473:pathology*; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D005070:Evans Blue; D005293:Ferritins; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D006937:Hypercholesterolemia / Q000473:pathology*; D008854:Microscopy, Electron; D011817:Rabbits
D002791:Cholesterol, Dietary; D005070:Evans Blue; D005293:Ferritins; D006735:Horseradish Peroxidase