One-step purification of L-type pyruvate kinase by microimmunoaffinity chromatography.
L-type pyruvate kinase from several tissues was purified by a micro-method of immunaffinity chromatography and the subunit structure analysed by SDS-electrophoresis (1-3). Whereas in adult human kidney L as well as L' seem to be present in the normal erythrocyte only L' subunits could be identified.
D000328:Adult; D000936:Antigen-Antibody Complex; D002846:Chromatography, Affinity / Q000379:methods; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007106:Immune Sera; D007527:Isoenzymes / Q000302:isolation & purification*; D007668:Kidney / Q000201:enzymology*; D046911:Macromolecular Substances; D008970:Molecular Weight; D011770:Pyruvate Kinase / Q000302:isolation & purification*
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007527:Isoenzymes; D046911:Macromolecular Substances; D011770:Pyruvate Kinase