[Morphological studies of the neurons of the area of the hypothalamic supraoptic nucleus in rats in diffusion chambers].
A method of diffusion chambers was used for hypothalamic tissue culturing. Chambers containing fragments from the supraoptic nucleus region of young rats-donors were implanted to rats-recipients. The processes of cell differentiation and interneuron interactions were studied by light and scanning electron microscopy. Morphological peculiarities of the main neuron types of the culture investigated suggest that the large differentiated multi- and bipolar neurons are the neurosecretory cells of the supraoptic nucleus. In the culture investigated small multipolar neurons of this nucleus and perinuclear areas were also present. The results give the possibility to consider the method of diffusion chambers as a promising one for the investigation of neurosecretory hypothalamic regions.
D000818:Animals; D002454:Cell Differentiation; D046508:Culture Techniques / Q000295:instrumentation; D007395:Interneurons / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000166:cytology*; D009490:Neurosecretory Systems / Q000166:cytology; D051381:Rats; D013495:Supraoptic Nucleus / Q000166:cytology*
Morfologicheskoe issledovanie neĭronov oblasti supraopticheskogo iadra gipotalamusa krys v diffuzionnykh kamerakh.