Chemical modification of the small intestinal Na+/D-glucose cotransporter by amino group reagents. Evidence for a role of amino group(s) in the binding of the sugar.
Some amino group reagents inactivate the small-intestinal Na+/D-glucose cotransporter, as measured either as a catalyst of Na+-dependent D-glucose transport or as a Na+-dependent phlorizin ligand. The amino group(s) studied in this paper are not identical with those investigated previously (Biber, J., Weber, J. and Semenza, G. (1983) Biochim. Biochim. Acta 728, 429-437): these are protected from inactivation by the simultaneous presence of Na+ plus sugar substrates. They are thus likely to be located within the substrate-binding site.
D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins / Q000378:metabolism*; D007421:Intestine, Small / Q000032:analysis* / Q000648:ultrastructure; D008871:Microvilli / Q000378:metabolism; D009004:Monosaccharide Transport Proteins; D010695:Phlorhizin / Q000378:metabolism; D011487:Protein Conformation / Q000187:drug effects; D011817:Rabbits
D002352:Carrier Proteins; D009004:Monosaccharide Transport Proteins; D010695:Phlorhizin