Incorporation of acylated wheat germ agglutinin into liposomes.
Purified wheat germ agglutinin (WGA) was derivatized with palmitic acid at an average stoichiometry of one fatty acid per dinner. Palmitoyl WGA was readily incorporated into liposomes with a cholate-dialysis method. Liposome-bound WGA caused agglutination of red blood cells at a concentration eight-fold lower than that of the native lectin. Furthermore, enhanced binding of liposome-bound WGA to mouse spleen cells was also observed. Potential applications of the liposome-bound lectin are discussed.
D000818:Animals; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D006386:Hemagglutination Tests; D066298:In Vitro Techniques; D037102:Lectins / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D013154:Spleen / Q000378:metabolism; D014909:Wheat Germ Agglutinins
D037102:Lectins; D008081:Liposomes; D014909:Wheat Germ Agglutinins