The pharmacological modulation of [3H]-disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion.
Lung slices from adult rats incubated in [methyl-3H]-choline chloride formed [3H]-disaturated phosphatidylcholine ( [3H]-DSPC) which was used as an index of lung surfactant. The slices were perifused after 3 h incubation in [methyl-3H]-choline chloride and the overflow of [3H]-DSPC, as a rate coefficient, was used as a measure of surfactant secretion. The basal overflow of [3H]-DSPC rapidly declined over the first 30 min of perifusion and then declined slowly. Salbutamol induced a prolonged, and sometimes delayed, increase in [3H]-DSPC overflow, which was reduced by (+/-)-propranolol. Potassium chloride produced an immediate, and usually transient, increase in [3H]-DSPC overflow which was not modified by atropine or (+/-)-propranolol. Adenosine 5'-triphosphate, but not phenylephrine, also increased [3H]-DSPC overflow. This method can measure the magnitude and time-course of lung surfactant secretion induced by drugs.
D000420:Albuterol / Q000494:pharmacology; D000818:Animals; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D011663:Pulmonary Surfactants / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D010713:Phosphatidylcholines; D011663:Pulmonary Surfactants; C017463:lecithins, disaturated; D014316:Tritium; D011189:Potassium Chloride; D011433:Propranolol; D007770:L-Lactate Dehydrogenase; D000420:Albuterol