[Amino acid sequence in the reactive site region of an acid-stable inhibitor of trypsin, chymotrypsin and intracellular proteinases from rabbit serum].
The thermo- and acid-stable trypsin, chymotrypsin and intracellular proteinases inhibitor (TAS-inhibitor) from rabbit serum was digested by trypsin, and its domain (Mr 6200) with antitryptic activity was obtained in homogeneous state. The N-terminal amino acid sequence of this domain was established by automatic Edman degradation: Thr-Val-Ala-Ala-Cys-Asx-Leu-Pro-Ile-Val-Pro-Gly-Pro-X-Arg-Gly-Ile-Phe-X- Leu-X-Ala-Phe-X-Ala-Val-X-Gly. A high degree of homology of the primary structures rabbit, human and bovine TAS-inhibitors was demonstrated.
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002850:Chromatography, Gel; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D006801:Humans; D011480:Protease Inhibitors / Q000097:blood*; D011817:Rabbits; D014361:Trypsin Inhibitors / Q000097:blood*
D011480:Protease Inhibitors; D014361:Trypsin Inhibitors; D002918:Chymotrypsin
Aminokislotnaia posledovatel'nost' v raĭone reaktivnogo tsentra kislotostabil'nogo ingibitora tripsina, khimotripsina i vnutrikletochnykh proteinaz iz syvorotki krovi krolika.