[Effect of chloroquine diphosphate on the superhelix structure of DNA and protein synthesis in synovial cells in chronic polyarthritis].
The influence of chloroquine diphosphate on protein synthesis in synovial fluid cells was investigated in 8 patients suffering from rheumatoid arthritis. Intraarticular injection of chloroquine diphosphate at a dose of 25 mg and 50 mg is followed a small decrease in incorporation of 3H-marked amino acids into the synovial fluid cells. Above a dose of 100 mg the protein synthesis is significant reduced. In a second experiment the supercoiled structure of DNA under C1Q was lost. After 2 hours the chain breaks were repaired.
D000596:Amino Acids / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000188:drug therapy*; D002738:Chloroquine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D004278:DNA, Superhelical / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007270:Injections, Intra-Articular; D008214:Lymphocytes / Q000187:drug effects*; D014176:Protein Biosynthesis; D013582:Synovial Fluid / Q000166:cytology / Q000187:drug effects*
D000596:Amino Acids; D004278:DNA, Superhelical; C023676:chloroquine diphosphate; D002738:Chloroquine
Uber den Einfluss von Chloroquindiphosphat auf die Superhelixstruktur der DNA und die Proteinsynthese der Synovialiszellen bei chronischer Polyarthritis.