title: Evaluation of reductive amperometric detection in the liquid chromatographic determination of antineoplastic platinum complexes.
abstract: The usefulness of reductive electrochemical detection at mercury drop electrodes has been determined for platinum complexes separated by solvent-generated anion-exchange high-performance liquid chromatography. Both current-sampled dropping mercury and hanging mercury drop electrodes (DME and HMDE) provide significant advantages over UV absorbance and off-line non-flame atomic absorption detection. The effects of chromatographic and polarographic parameters on analytical system performance have been investigated. By raising the detector cell temperature, the detector response to cis-dichlorodiammineplatinum(II) (DDP) can be shifted anodically to 0.0 V vs. Ag/AgCl, thereby increasing detector selectivity for this compound. The noise-limited minimum detectable quantities of DDP with DME and HMDE are 1.8 ng and 70 pg injected, respectively. DDP can be determined in untreated urine at levels below 100 ng/ml.
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange; D002945:Cisplatin / Q000031:analogs & derivatives / Q000627:therapeutic use / Q000652:urine*; D004563:Electrochemistry; D005069:Evaluation Studies as Topic; D006801:Humans; D010084:Oxidation-Reduction; D010984:Platinum / Q000652:urine
chemical_list: D010984:Platinum; D002945:Cisplatin