Production of antibody for alpha 1-acid glycoprotein using carcinoembryonic antigen and normal fecal antigen.
The structural characteristics of carcinoembryonic antigen (CEA) and CEA-like antigen in feces and meconium were examined using antibody raised against 2 antigens. When antibody to CEA was made, anti-alpha 1-acid glycoprotein (AG) (nonprecipitating antibody) besides the precipitating antibody for CEA was made. When antibodies against each CEA-like antigen (mol. wt. 180,000) purified from feces and meconium were tested, each antibody showed a fused precipitin line for 2 antigens. Some antibodies showed the non-precipitating antibody for AG. CEA and CEA-like antigen in feces and meconium may be composed of 2 portions (AG portion and non-AG portion). Antibody specific to these antigens may be the antibody directed to the non-AG portion.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D002272:Carcinoembryonic Antigen / Q000008:administration & dosage / Q000276:immunology*; D005243:Feces / Q000276:immunology; D006801:Humans; D007114:Immunization; D008470:Meconium / Q000276:immunology; D008970:Molecular Weight; D009961:Orosomucoid / Q000276:immunology*; D011863:Radioimmunoassay
D000906:Antibodies; D000941:Antigens; D002272:Carcinoembryonic Antigen; D009961:Orosomucoid; C035221:fecal antigen-1, normal