Assay for monoamine oxidases A and B by high-performance liquid chromatography with fluorescence detection.
A sensitive method for the assay of monoamine oxidases A and B is described which employs high-performance liquid chromatography with fluorescence detection. Rat brain mitochondria were used as a preparation of the enzymes. p-Sulfamoylbenzaldehyde and benzaldehyde formed enzymatically from p-sulfamoylbenzylamine (the substrate of monoamine oxidase A) and benzylamine (the substrate of monoamine oxidase B), respectively, are converted simultaneously into fluorescent compounds with 2,2'-dithiobis(1-aminonaphthalene). These compounds are separated by reversed-phase chromatography on mu Bondapak CN. The limits of detection for p-sulfamoylbenzaldehyde and benzaldehyde formed enzymatically are 30 and 10 pmol per assay tube, respectively.
D000818:Animals; D001547:Benzaldehydes; D001921:Brain / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D008995:Monoamine Oxidase / Q000032:analysis*; D051381:Rats; D013050:Spectrometry, Fluorescence; D000096926:Benzenesulfonamides
D001547:Benzaldehydes; D008995:Monoamine Oxidase; C040355:4-sulfamoylbenzaldehyde; D000096926:Benzenesulfonamides; C032175:benzaldehyde