Characterization of testosterone binding protein of rat liver cytosol.
Testosterone binding protein from rat liver cytosol, which had been incubated with [3H]testosterone followed by treatment with dextran-coated charcoal, was analyzed by DEAE-cellulose and phosphocellulose chromatography. On DEAE-cellulose chromatography, two distinct peaks of radioactivity were eluted at 0.07 M and 0.19 M KCl, both sedimented in 4 S regions. Phosphocellulose chromatography resulted in a broad peak at 0.08 M KCl, with a shoulder at 0.04 M KCl, both sedimented at 4 S. These findings indicated that testosterone binding protein consists of two types of components each with 4 S.
D000727:Androgen-Binding Protein / Q000032:analysis*; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D003600:Cytosol / Q000032:analysis; D008099:Liver / Q000032:analysis*; D008297:Male; D008982:Molybdenum / Q000494:pharmacology; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000378:metabolism*
D000727:Androgen-Binding Protein; D002352:Carrier Proteins; D013739:Testosterone; D010664:Phenylmethylsulfonyl Fluoride; D008982:Molybdenum