[Isolation of fractions containing natriuretic hormone from blood plasma and their testing in in vivo and in vitro experiments].
Natriuretic hormone (NUH) was isolated from the canine arterial blood plasma by means of gel chromatography on sefadex G-25. The plasma was preliminarily deproteinized with trichloro-acetic acid and the supernatant layer was lyophilized. Five peptide fractions were obtained by means of spectrophotometry in UV spectrum at 280 microns. The 2nd and 3d fractions of this material had a natriuretic activity in experiments on rats. The same fractions inhibited short-circuit current and the frog skin potential, as well as the frog skin oxygen consumption. For the present it is unlikely to confirm that this natriuretic activity should be caused by one or different chemical substances due to inaccurate separation of the 2nd and 3d fractions. The methods of a study of short-circuit current and the frog skin potential as well as a polarographic study of the frog skin oxygen consumption can be used for NUH testing.
D000818:Animals; D001158:Arteries; D001692:Biological Transport / Q000187:drug effects; D001798:Blood Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D002850:Chromatography, Gel; D004285:Dogs; D004573:Electrolytes / Q000378:metabolism; D066298:In Vitro Techniques; D009318:Natriuresis; D045283:Natriuretic Agents; D010101:Oxygen Consumption / Q000187:drug effects; D011506:Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D011898:Ranidae; D051381:Rats; D012867:Skin / Q000187:drug effects / Q000378:metabolism
D001798:Blood Proteins; D004573:Electrolytes; D045283:Natriuretic Agents; D011506:Proteins
Vuydelenie iz plazmy krovi fraktsiĭ, soderzhashchikh natriĭureticheskiĭ gormon, i ikh testirovanie v opytakh in vivo i in vitro.