Catabolism of 6 keto PGE1: biological activation by the rat kidney in vitro.
An increase in anti-aggregatory but not spasmogenic activity was observed when 6 keto prostaglandin E1 (but not PGE1) was incubated at 37 degrees C with rat kidney 100 000 X g supernatant. No such biological activation was observed in boiled rat kidney supernatant. After high-pressure liquid chromatography two absorbance peaks with anti-aggregatory activity were detected. One peak had a retention time identical to authentic 6 keto prostaglandin E1 whilst the second peak did not coincide with known anti-aggregatory prostaglandins.
D000527:Alprostadil / Q000031:analogs & derivatives*; D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism*; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects; D011458:Prostaglandins E / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D011458:Prostaglandins E; C022950:6-ketoprostaglandin E1; D000527:Alprostadil