Effects of Wratizolin on growth and metabolism of cells in vitro.
The new isothiazole derivative--Wratizolin was found to have several properties which are characteristic of the acidic non-steroidal antiinflammatory drugs, among others--indomethacin. The minimal cytotoxic concentration (MCC) of Wratizolin for the chicken or mouse embryonic monolayer tissue cultures maintained in the media without serum is 6.25 microM. In the medium containing from 3 to 10% calf serum MCC for several animal cells was found to be from 12.5 to 62.5 microM. Wratizolin inhibited the synthesis of DNA in the cultured human lymphocytes at the subtoxic concentration only. Wratizolin, in a characteristic way, stimulates the synthesis of lactic acid and increases the consumption of glucose by the chick or mouse fibroblasts. The drug at concentration of 10(-6) to 10(-12) M stimulates the multiplication of mouse L cells. In all above tests Wratizolin is more active than indomethacin.
D000650:Amnion; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects* / Q000378:metabolism; D002642:Chick Embryo; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology*; D007668:Kidney; D007773:Lactates / Q000096:biosynthesis; D019344:Lactic Acid; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D013552:Swine; D013844:Thiazoles / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D007773:Lactates; D013844:Thiazoles; D019344:Lactic Acid; C027999:5-(benzoylamino)-N-(4-chlorophenyl)-3-methyl-4-isothiazolecarboxamide; D004247:DNA; D005947:Glucose; D013936:Thymidine; D007213:Indomethacin