Release of a 6-oxoprostaglandin E1-like substance from human platelets.
1. Human platelet-rich plasma incubated at 37 degrees C generates an antiaggregatory prostaglandin which is spasmogenic on gastrointestinal smooth muscle. 2. Platelet-rich plasma from female donors generated more biological activity and was more sensitive to the anti-aggregatory activity of added prostaglandin I2 (PGI2) compared with that from age-matched male controls. 3. After thin-layer chromatography of extracted platelet-rich plasma, biological activity was detected in a zone which co-chromatographed with 6-oxoprostaglandin E1. 4. Neither extracted platelet-rich plasma nor authentic 6-oxoprostaglandin E1 were inactivated following incubation with purified 15-hydroxyprostaglandin dehydrogenase. 5. The relevance of these findings for regulating platelet reactivity is discussed.
D000328:Adult; D000527:Alprostadil / Q000031:analogs & derivatives*; D001792:Blood Platelets / Q000378:metabolism*; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D005260:Female; D006801:Humans; D008297:Male; D010974:Platelet Aggregation; D011458:Prostaglandins E / Q000378:metabolism*; D012737:Sex Factors
D011458:Prostaglandins E; C022950:6-ketoprostaglandin E1; D000527:Alprostadil