Production of a fibrinolytic enzyme from cultures of guinea pig keratocytes grown on microcarriers.
Microcarrier technology was investigated as a means of producing a fibrinolytic enzyme, for the treatment of occlusive thrombotic vascular disorders, in sufficient quantity to carry out an evaluation of its clinical potential and the economics of large scale production. Preliminary results showed that the cells grew well and produced significant quantities of the enzyme at high (10 g/1) microcarrier concentrations.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003238:Connective Tissue / Q000201:enzymology; D003239:Connective Tissue Cells; D003315:Cornea / Q000166:cytology / Q000201:enzymology*; D006168:Guinea Pigs; D008863:Microspheres