Thrombin-induced shape changes of cultured endothelial cells: metabolic and functional observations.
Cultured human umbilical vein endothelial cells (EC) incubated in the presence of 2-deoxy-D-glucose (20 mmol/l) or at 4 degrees C lost their ability to undergo shape changes when exposed to thrombin (1 N.I.H. u/ml). Drugs blocking Ca++-flux (verapamil and nifedipin), microfilament disrupting agents (cytochalasin B and D) and microtubule disrupting agents (colchicine and colcemid) did not prevent thrombin-induced shape changes. None of the agents tested inhibited the accelerated thrombin-induced 51Cr-release from the cells. Pretreatment of EC with thrombin did not influence their ability to mediate clot retraction.
D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003018:Clot Retraction; D003078:Colchicine / Q000494:pharmacology; D003572:Cytochalasins / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects; D004727:Endothelium / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D008870:Microtubules / Q000187:drug effects; D009543:Nifedipine / Q000494:pharmacology; D013696:Temperature; D013917:Thrombin / Q000494:pharmacology*; D014471:Umbilical Veins; D014700:Verapamil / Q000494:pharmacology
D003572:Cytochalasins; D014700:Verapamil; D013917:Thrombin; D009543:Nifedipine; D003078:Colchicine; D002118:Calcium