A novel polyacrylamide-type support prepared by p-benzoquinone activation.
A new and simple method for the activation of polyacrylamide gels using p-benzoquinone is described. The optimal conditions of activation have been elaborated. The activated support could be successfully applied to the immobilization of ligands having nucleophilic groups active over a broad pH range.
D000180:Acrylic Resins; D016227:Benzoquinones; D002374:Catalase / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D007202:Indicators and Reagents; D007700:Kinetics; D008024:Ligands; D011809:Quinones; D014357:Trypsin / Q000378:metabolism
D000180:Acrylic Resins; D016227:Benzoquinones; D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D008024:Ligands; D011809:Quinones; C004532:quinone; C032004:Akrilex C; D005949:Glucose Oxidase; D002374:Catalase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D014357:Trypsin; D005634:Fructose-Bisphosphate Aldolase