title: Leumorphin is a novel endogenous opioid peptide derived from preproenkephalin B.
abstract: Using synthetic leumorphin, we obtained antisera for leumorphin and set up two radioimmunoassays (RIAs) with different specificities. Gel exclusion chromatography coupled with the two RIAs showed the existence of a considerable amount of leumorphin-like peptide in water extracts from porcine neuro-intermediate pituitaries. Reverse phase high performance liquid chromatography revealed that leumorphin-like peptide in the water extracts was indistinguishable from synthetic leumorphin. These results along with potent opioid activity of leumorphin indicate that leumorphin is a novel endogenous opioid peptide derived from preproenkephalin B.
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004399:Dynorphins; D004591:Electrophoresis, Polyacrylamide Gel; D004723:Endorphins; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000032:analysis; D004745:Enkephalins / Q000032:analysis*; D010902:Pituitary Gland / Q000378:metabolism; D011498:Protein Precursors / Q000032:analysis*; D011863:Radioimmunoassay; D013552:Swine
chemical_list: D004723:Endorphins; D004745:Enkephalins; D011498:Protein Precursors; D004743:Enkephalin, Leucine; D004399:Dynorphins; C036200:rimorphin; C033660:preproenkephalin