Metabolic labelling of oligosaccharide chains of glycoproteins in a rat myoblast cell line and its lectin resistant mutants.
We have determined the experimental conditions under which optimum hydrolysis of the various oligosaccharides of membrane glycopeptides can be achieved in a permanent line of rat skeletal myoblasts and its wheat germ agglutinin resistant mutants. The labelled glycopeptides were isolated by extensive pronase digestion of whole cells. Labelling studies demonstrated that label from fucose and galactose remained largely in these two sugars, while glucosamine labelled sialic acid and N-acetylglucosamine. Mannose label was found in both mannose and fucose.
D000107:Acetylation; D000818:Animals; D002460:Cell Line; D055598:Chemical Phenomena; D002621:Chemistry; D004351:Drug Resistance; D006023:Glycoproteins / Q000378:metabolism*; D037102:Lectins / Q000494:pharmacology*; D009132:Muscles; D009154:Mutation; D009844:Oligosaccharides / Q000378:metabolism*; D051381:Rats; D014909:Wheat Germ Agglutinins
D006023:Glycoproteins; D037102:Lectins; D009844:Oligosaccharides; D014909:Wheat Germ Agglutinins