[Effects of a fixed mixture of lactate, glucose and phosphate on the survival of human spermatozoa].
It is admitted that selection of human motile spermatozoa improves chances of conception by homologous artificial insemination. Effect of media constituted by different concentrations of glucose, lactate and phosphate in the maintenance of vitality and forward motility (positively correlated with penetrating capacity) was studied in human spermatozoa after washing. Lactate optimal concentrations appear necessary in the maintenance of progressive motility. The vitality is little affected in the various media. To complete this minimum medium various substances known to improve spermatozoa survival should be added. This complete media should allow to dispose of suitable spermatozoa suspensions during several days permitting many inseminations by cycle.
D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007773:Lactates / Q000494:pharmacology*; D008297:Male; D010710:Phosphates / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*
D007773:Lactates; D010710:Phosphates; D005947:Glucose
Effet d'un mélange déterminé de lactate, glucose et phosphate sur la survie des spermatozoïdes humains.