Promotion of epithelial cell adhesion on collagen by proteins from rat embryo fibroblasts.
We have found that the promoting effects of proteins from fibroblast conditioned medium (PCM) promote adhesion of rat liver epithelial cells (nonmalignant RL-34 cell line) seeded on collagen coated substratum. Since the effect was completely inhibited by treating the cells with cycloheximide the protein(s) are probably produced by the RL-34 cells. We also found that PCM-A fraction which is identical to fibronectin purified from whole PCM had no effect on RL-34 cell adhesion and that the adhesion was not inhibited even by adding affinity purified anti-FN antibody to the medium containing FN-free PCM (PCM-B fraction). This indicates that adhesion of rat liver epithelial cells on collagen substratum does not require FN, but requires the adhesion promoting factor(s) existing in PCM-B.
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D003094:Collagen; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D004622:Embryo, Mammalian; D004847:Epithelial Cells; D005347:Fibroblasts; D005353:Fibronectins / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology*; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D003470:Culture Media; D005353:Fibronectins; D011506:Proteins; D003094:Collagen; D003513:Cycloheximide