title: Identification of specific plasma proteins determining the agarose gel electrophoresis by the immunosubtraction technique.
abstract: The immunosubtraction technique was used to identify the proteins in electrophoretically separated plasma. The technique consists of forcing the plasma through a layer of monospecific antibody purified by electrophoresis; the specific protein is thereby precipitated and is consequently absent from the subsequent electrophoretogram. This technique was successfully applied to the following proteins (in order of distance from the anode): prealbumin, albumin, alpha-lipoproteins, alpha 1-antitrypsin, Gc-globulins, alpha 2-macroglobulin, haptoglobin, fibronectin, transferrin, beta-lipoproteins, C3, fibrinogen. The method may be applied to the study of protein polymorphism.
mesh_terms: D000906:Antibodies; D000936:Antigen-Antibody Complex; D001798:Blood Proteins / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D006801:Humans; D007118:Immunoassay
chemical_list: D000906:Antibodies; D000936:Antigen-Antibody Complex; D001798:Blood Proteins