title: A simplified technique for fertilization and culture of human preimplantation embryos in vitro.
abstract: The paper describes simplification of existing methods of culture in human IVF. Focusing attention on the need to streamline procedures, the mode of housing the egg/embryo in a constant accessible environment is examined. We now culture with B2/B3-Menezo medium in a multidish under 5% CO2 in air at 37 degrees C in an open system within a CO2 gassing incubator. The advent of the multidish as a specific housing system is highlighted. The 80% fertilization rate combined with a stable osmolarity demonstrates that previous success is maintained with changes introduced to improve convenience and simplicity of procedures.
mesh_terms: D000328:Adult; D003470:Culture Media; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007247:Infertility, Female / Q000628:therapy*; D011247:Pregnancy
chemical_list: D003470:Culture Media