[Computer bank of amino acid sequences of protein hormones. Comparative analysis of the sequences in pro-opio-melanocortin, proenkephalin and prodynorphin: detection of phorphin--the 4th repeating peptide in prodynorphin].
Using a BESM-6 computer, a computer system for accumulation and comparative analysis of amino acid sequences (AS) of protein-peptide hormones and their precursors (the so-called computer bank of protein hormone AS) was developed. A Fortran-based program designed for construction of correspondence schemes of AS and their local similarity profiles was elaborated. In combination with the previous programs this system allows a rapid inclusion of the newly deciphered sequences into the corresponding homologous groups, thus complementing the correspondence scheme and specifying the evolution profiles. A comparative analysis of AS in proopiomelanocortin (POMC), proenkephalin (PENK) and prodynorphin (PDIN) revealed evolutionary-conservative and variable sites. The conservative sites of AS are active centers of the hormones. The leu-enkephalin analog, phorphin, the fourth repeating peptide of this precursor, was detected in prodynorphin, which, similar to beta-neo-endorphin, dynorphin and rimorphin may possess a biological activity. The similarity of the effector sites of the melanotropin sequence from POMC to the met-enkephalin sequence from PENK and leu-enkephalin sequence from PDIN as well as to gastrin and cholecystokinin was established. This may suggest that the hormone-receptor complexes in target organs of these hormones are also similar.
D000595:Amino Acid Sequence; D003201:Computers; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives* / Q000032:analysis; D004745:Enkephalins; D010908:Pituitary Hormones, Anterior; D011333:Pro-Opiomelanocortin; D011498:Protein Precursors; D012984:Software
D004745:Enkephalins; D010908:Pituitary Hormones, Anterior; D011498:Protein Precursors; C029992:proenkephalin; D004743:Enkephalin, Leucine; D011333:Pro-Opiomelanocortin; C040908:phorphin; C033660:preproenkephalin
Komp'iuternyĭ bank aminokislotnykh posledovatel'nosteĭ belkovykh gormonov. Sravnitel'nyĭ analiz posledovatel'nosteĭ v gruppe proopiomelanokortina, proénkefalina i prodinorfina: obnaruzhenie forfina--chetvertogo povtoriaiushchegosia peptida v prodinorfine.