The inhibition of hepatic S-3-hydroxy-3-methylglutaryl-CoA reductase by 3,3,5-trimethylcyclohexanol and its mandelic acid ester, cyclandelate.
Rat hepatic HMGCoA reductase was found to be at least 50% inhibited 17 hr after administration of a single oral dose of 3,3,5-trimethylcyclohexanyl mandelate (cyclandelate), a vasoactive substance. This inhibition was also found in rats given the 3,3,5-trimethylcyclohexanol component but only slight inhibition was seen after an equimolar dose of mandelate. The inhibition of HMGCoA was observed both around the high point and near the low point of the diurnal activity cycle. The effect did not persist to 41 hr after treatment. There was no direct inhibition of HMGCoA reductase by trimethylcyclohexanol when added to the assay system in vitro. The in vivo effect of these inhibitors was specific for HMGCoA reductase. There was no change, neither elevation nor depression, of the amount of microsomal membrane components cytochromes b5 and P-450, not was the activity of another microsomal enzyme, arylesterase, affected by dosing with cyclandelate or trimethylcyclohexanol.
D000284:Administration, Oral; D000818:Animals; D002940:Circadian Rhythm; D003495:Cyclandelate / Q000494:pharmacology*; D003511:Cyclohexanols / Q000494:pharmacology*; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D008297:Male; D008333:Mandelic Acids / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D003511:Cyclohexanols; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D008333:Mandelic Acids; C037269:3,3,5-trimethylcyclohexanol; D003495:Cyclandelate