An artifact in measurements of in vivo light-induced absorbance changes.
The effects of scattered actinic radiation on photomultipliers (Hamamatsu R-562) were investigated. Using cotton-wool to model dense biological preparations, it was found that the scattered actinic radiation received by the photomultiplier gives rise to phytochrome-like signals. This demonstrated the necessity to shield the photomultiplier from scattered actinic light for sensitive measurements with light-scattering preparations.
D008027:Light; D010834:Phytochrome / Q000032:analysis; D012542:Scattering, Radiation; D013053:Spectrophotometry / Q000295:instrumentation
D010834:Phytochrome