The effect of adriamycin on Z-DNA formation and DNA synthesis.
The ability of adriamycin to inhibit Z-DNA formation induced by a high-salt environment was investigated. ADM inhibited this conversion, such that in poly (dG-dC) total inhibition was observed at 1 ADM: 9 base pairs and in eukaryotic DNA (calf thymus) at 1 ADM: 11,5 base pairs. Even at low ADM concentration, 1 ADM: 160 base pairs, some inhibition was observed. At similar ADM:DNA concentrations, an inhibition in DNA synthesis in cells in culture was observed, which showed some parallel with the inhibition of Z-DNA formation. A model is proposed where Z-DNA formation precedes DNA synthesis and where inhibition of the former could explain the antineoplastic nature of adriamycin.
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004247:DNA / Q000235:genetics*; D004261:DNA Replication / Q000187:drug effects*; D004317:Doxorubicin / Q000494:pharmacology*; D007700:Kinetics; D009690:Nucleic Acid Conformation / Q000187:drug effects; D009994:Osmolar Concentration; D013950:Thymus Gland
D004317:Doxorubicin; D004247:DNA