[Diagnosis of Vibrio cholerae in the laboratory].
The authors describe a new specific and immunologic process for V. cholerae isolation. Specific anti-V. cholerae antibodies (specific IgG anti-fraction Ch 1 + 2) are sticked on magnetic beads. These beads are added to choleric stool. V. cholerae germs stick on the beads. These particles are drawn out of the mixture with the help of a magnet and laid on a Mueller-Hinton agar plate. Incubation is carried out for 18 hours. One drop of toluen is then added on the beads. Wells are sinked in the agar plate and filled with specific serum or antitoxin serum. 12 hours after, the appearance of a precipitation line, facing the specific serum, reveals the presence of toxinogen V. cholerae.
D000372:Agglutination Tests / Q000379:methods; D002771:Cholera / Q000175:diagnosis*; D005243:Feces / Q000382:microbiology; D005516:Food Microbiology; D006801:Humans; D014734:Vibrio cholerae / Q000032:analysis* / Q000276:immunology; D014871:Water Microbiology
Diagnostic du vibrion cholérique au laboratoire.