Synthesis of components of the cytochrome b-f complex by isolated pea chloroplasts.
Three components of the cytochrome b-f complex, cytochrome f, cytochrome b-563 and a 15.2-kDa polypeptide, were labelled with radioactive amino acids in isolated pea chloroplasts incubated in the light with added Mg-ATP. The assembly of cytochrome b-563 (19.5 kDa) into the cytochrome complex required the presence of added Mg-ATP whereas cytochrome f (37.3 kDa) and the 15.2-kDa polypeptide were assembled in its absence. Incorporation of [35S]methionine into the polypeptide chain of cytochrome b-563 and the 15.2-kDa component was confirmed by peptide mapping of the products of partial digestion with papain.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D002736:Chloroplasts / Q000201:enzymology*; D003580:Cytochromes / Q000096:biosynthesis*; D045348:Cytochromes f; D007887:Fabaceae / Q000201:enzymology; D008715:Methionine / Q000378:metabolism; D010446:Peptide Fragments / Q000096:biosynthesis; D010940:Plant Proteins / Q000096:biosynthesis; D010946:Plants, Medicinal; D012270:Ribosomes / Q000378:metabolism
D000596:Amino Acids; D003580:Cytochromes; D010446:Peptide Fragments; D010940:Plant Proteins; D000255:Adenosine Triphosphate; D045348:Cytochromes f; D008715:Methionine