Treatment of mobile phase particulate matter in low-angle quasi-elastic light scattering.
The method of quasi-elastic laser light scattering (QLS), particularly at low forward scattering angles, has been complicated by the transient presence of Mie or large Rayleigh scattering particles which contaminate the scattering volume. These large contaminating particles have substantial effects on photon correlation spectroscopy because the presence of these larger scatterers tends to decrease the value of the apparent diffusion coefficient of the particle of interest. A method is presented which yields more accurate diffusion constants by autocorrelation of selected photon count periods representative of minimal Mie or large Rayleigh particle contamination. This method was applied to the determination of the apparent diffusion constant for four proteins-ovalbumin, chymotrypsinogen-A, bovine serum albumin, and ribonuclease-A.
D000818:Animals; D002417:Cattle; D002919:Chymotrypsinogen / Q000032:analysis; D004058:Diffusion; D008027:Light; D010047:Ovalbumin / Q000032:analysis; D012259:Ribonuclease, Pancreatic / Q000032:analysis; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000032:analysis; D012996:Solutions
D012996:Solutions; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D002919:Chymotrypsinogen; D012259:Ribonuclease, Pancreatic