title: Chloroquine treatment does not cause phospholipid storage by depleting rat liver lysosomes of acid phospholipase A.
abstract: Chronic treatment of rats with chloroquine causes phospholipid storage in the lysosomes of liver and other tissues. This could be due to chloroquine-induced depletion of liver lysosomal phospholipase A. Alternatively, it could be caused by chloroquine inhibition of intralysosomal phospholipid catabolism. We treated rats with chloroquine in a dosage schedule sufficient to cause a 35% increase in liver phospholipid content. Acid phospholipase A activity was increased in liver homogenates and in lysosomal preparations obtained from chloroquine-treated animals. Thus, while fibroblasts respond to chloroquine treatment by cellular depletion of certain acid hydrolases as shown by others, the levels of acid phospholipase A increase in liver. Our results provide additional new support for the hypothesis that inhibition of lysosomal phospholipase A activity is the major mechanism of chloroquine-induced phospholipidosis.
mesh_terms: D000818:Animals; D002738:Chloroquine / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats
chemical_list: D010743:Phospholipids; D002738:Chloroquine; D010740:Phospholipases; D010741:Phospholipases A