Selection of therapeutic agents for intraocular proliferative disease. Cell culture evaluation.
A variety of antimetabolites, steroids, and nonsteroidal anti-inflammatory agents were tested for their ability to inhibit rabbit dermal and conjunctival fibroblast proliferation in cell culture. Doxorubicin hydrochloride and fluorouracil produced notable inhibition in concentrations of less than 1 mg/L. Meclofenamate sodium and indomethacin produced notable inhibition at concentrations of 11 and 40 mg/L, respectively. Dexamethasone sodium phosphate and triamcinolone acetonide produced inhibition at 200 and 150 mg/L, respectively, but paradoxically increased proliferation almost two-fold at concentrations ranging from 1 to 30 mg/L under identical culture conditions. Methotrexate sodium demonstrated only limited effectiveness. This assay system may be a useful approach to drug selection in the treatment of a variety of ocular proliferative disorders. Fluorouracil may prove to be of significant value in the treatment of intraocular proliferative disorders.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000963:Antimetabolites / Q000494:pharmacology*; D002455:Cell Division; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D005123:Eye / Q000187:drug effects*; D005128:Eye Diseases / Q000188:drug therapy; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D007213:Indomethacin / Q000494:pharmacology; D008469:Meclofenamic Acid / Q000494:pharmacology; D008727:Methotrexate / Q000494:pharmacology; D011817:Rabbits; D014221:Triamcinolone / Q000494:pharmacology
D000893:Anti-Inflammatory Agents; D000963:Antimetabolites; D014221:Triamcinolone; D008469:Meclofenamic Acid; D003907:Dexamethasone; D004317:Doxorubicin; D007213:Indomethacin; D008727:Methotrexate