Detection of H-Y in the enzyme-linked immunosorbent assay.
We have developed a new enzyme-linked immunosorbent assay for determination of H-Y phenotype in the human. This assay, which measures the inhibition of the reaction of a monoclonal anti-H-Y antibody and a mouse testis extract as a source of H-Y antigen, was applied to the supernatant of lymphocytes from ten normal male and ten normal female subjects. Introduction of supernatant from male cells gave readings of 69%-78% of those obtained with testis supernatant alone; female-cell supernatant did not inhibit the reaction (89%-102%).
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006182:H-Y Antigen / Q000032:analysis*; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D010641:Phenotype; D013737:Testis / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D006182:H-Y Antigen