[Radiation destruction of DNA and histone in the composition of the chromatin].
A study was made of the radiolysis of low-molecular-weight chromatin in water solution (0.037%) at 273 K in the absence of O2. It was demonstrated by the gel-electrophoresis method that both fragments of the complex are destructed and polymers formed involving DNA and protein. In other words, "DNA-protein" cross-links are formed. The yield of these processes essentially decreases if the chromatin solution is irradiated in the presence of inositol (0.05 M), an OH radical acceptor. The formation of cross-links is connected with the recombination of macroradicals in DNA and histone or with the reaction of their conversion products with the functional groups of the chromatin fragments.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002843:Chromatin / Q000032:analysis / Q000528:radiation effects*; D004247:DNA / Q000032:analysis / Q000528:radiation effects*; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000032:analysis / Q000528:radiation effects*; D066298:In Vitro Techniques; D008099:Liver / Q000528:radiation effects; D051379:Mice; D012996:Solutions
D002843:Chromatin; D006657:Histones; D012996:Solutions; D004247:DNA
Radiatsionnaia destruktsiia DNK i gistona v sostave khromatina.