Effect of atractylosides, palmitoyl coenzyme A, and anion transport inhibitors on translocation of nucleotide sugars and nucleotide sulfate into Golgi vesicles.
The effect of palmitoyl coenzyme A, atractylosides, and anion transport inhibitors on translocation into rat liver Golgi vesicles of adenosine 3'-phosphate 5'-phosphosulfate (PAPS), CMP-N-acetylneuraminic acid, and GDP-fucose was studied. Translocation of the above three nucleotide derivatives was inhibited by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS; 50% inhibition required 10-20 microM DIDS). The inhibition of translocation of PAPS by DIDS was used to demonstrate that sulfation of macromolecules within Golgi vesicles is preceded by translocation of PAPS into the vesicles. Palmitoyl coenzyme A, at concentrations below its critical micellar concentration, specifically inhibited translocation into Golgi vesicles of PAPS but not CMP-NeuAc and GDP-fucose. Inhibition of PAPS translocation by 50% required 9 microM palmitoyl coenzyme A. Translocation of PAPS but not of CMP-NeuAc or GDP-fucose was also inhibited by atractyloside or carboxyatractyloside with 50% inhibition requiring 15 microM either glycoside. This pattern of inhibition suggests structural similarities between the putative translocator of PAPS in Golgi membranes and the ATP/ADP translocator of mitochondria.
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000214:Acyl Coenzyme A / Q000494:pharmacology*; D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology*; D001865:Bongkrekic Acid / Q000494:pharmacology; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid / Q000378:metabolism*; D006027:Glycosides / Q000494:pharmacology*; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism*; D006154:Guanosine Diphosphate Fucose / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D010171:Palmitoyl Coenzyme A / Q000494:pharmacology*; D010724:Phosphoadenosine Phosphosulfate / Q000378:metabolism*; D051381:Rats; D012794:Sialic Acids / Q000378:metabolism*
D000214:Acyl Coenzyme A; D000227:Adenine Nucleotides; D006027:Glycosides; D009702:Nucleoside Diphosphate Sugars; D012794:Sialic Acids; D001865:Bongkrekic Acid; D006154:Guanosine Diphosphate Fucose; D010171:Palmitoyl Coenzyme A; D001278:Atractyloside; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid; D010724:Phosphoadenosine Phosphosulfate; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; C003853:carboxyatractyloside