Effect of C3 depletion on experimental Pseudomonas aeruginosa ocular infection: histopathological analysis.
The ocular histopathological response of C3-depleted and normal DBA/2J mice was compared after corneal scarification and experimental infection with topically applied Pseudomonas aeruginosa. Normal, non-C3-depleted mice were capable of mounting a vigorous polymorphonuclear leukocyte response at 24 h after bacterial challenge and eventually restored corneal clarity. In contrast, C3-depleted animals responded at 24 h to the ocular bacterial challenge with a decreased number of polymorphonuclear leukocytes migrating into the cornea. Additionally, the eyes of C3-depleted mice exhibited persistence of bacteria, cataract of the ocular lens, and loss of vision.
D000818:Animals; D003176:Complement C3 / Q000378:metabolism*; D003315:Cornea / Q000648:ultrastructure; D003316:Corneal Diseases / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D003886:Descemet Membrane / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D005260:Female; D007113:Immunity, Innate; D051379:Mice; D008811:Mice, Inbred DBA; D009504:Neutrophils / Q000473:pathology / Q000648:ultrastructure; D011552:Pseudomonas Infections / Q000235:genetics / Q000276:immunology* / Q000473:pathology
D003176:Complement C3