title: Isomyosin distribution in skeletal muscles of normal and myotonic goats.
abstract: Isoforms of myosin were examined in hindleg muscles isolated from normal and myotonic goats. The muscles studied were the soleus, gastrocnemius, gluteus accessorius, semitendinosus, semimembranosus, and adductor. The isomyosins were analyzed by pyrophosphate polyacrylamide gel electrophoresis. The relative proportions of slow and fast myosin isoforms present were determined by densitometric scanning of Coomassie Blue-stained gels. All muscles contained three fast myosin isoforms and either one or two slow myosin isoforms. In normal goat, the soleus and gastrocnemius, containing 70%-75% slow isomyosins, were representative of slow muscle. The semimembranosus, the semitendinosus, and the adductor, with more than 50% fast myosin, represented a predominantly fast muscle group. The gluteus accessorius, with approximately 45% fast myosin isoforms, was intermediate between the other two groups. In the myotonic goat, a consistent and significant increase in the proportion of fast isomyosins was observed for all the muscles studied. The largest change occurred in the gastrocnemius where the incremental increase in the percentage of fast isomyosins was over 30%. All the other muscles examined had incremental increases in fast isomyosin content which ranged from 16.1% to 22.0%. These results suggest that the abnormal action potential pattern of myotonia leads to a redistribution of the myosin isoforms.
mesh_terms: D000200:Action Potentials; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats; D006801:Humans; D009119:Muscle Contraction; D009132:Muscles / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism*; D009222:Myotonia / Q000378:metabolism / Q000662:veterinary*; D009224:Myotonia Congenita / Q000378:metabolism / Q000662:veterinary*
chemical_list: D009218:Myosins