Quantitative gas-liquid chromatography of taurine.
A method has been developed for the determination of taurine by gas-liquid chromatography. The method involves the conversion of taurine into its N-isobutyloxycarbonyl di-n-butylamide derivative and chromatography on a 1.5% OV-17 column. The derivative can be prepared in quantitative yield, having good chromatographic properties. The calibration curve for taurine in the range 5-500 nmol was linear and sufficiently reproducible for quantitative determination. Complex biological material such as urine can be analysed accurately and precisely by this method without prior clean-up of the sample.
D000328:Adult; D055598:Chemical Phenomena; D002621:Chemistry; D002648:Child; D002849:Chromatography, Gas / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D013654:Taurine / Q000031:analogs & derivatives / Q000032:analysis* / Q000652:urine; D013997:Time Factors
D013654:Taurine; C041256:N-isobutyloxycarbonyl-di-n-butylamide-taurine