Higher order structure in a short repeat length chromatin.
Polynucleosomes from calf brain cortical neurone nuclei have an average repeat length of less than 168 base pairs. The ability of this material to adopt higher order structure has been assessed by various physical techniques. Although containing on average less DNA per nucleosome than is required to form a chromatosome, this short repeat length chromatin folded in an H1 dependent manner to a structure with properties similar to those observed for longer repeat length chromatins such as that of chicken erythrocyte (McGhee, J.D., D.C. Rau, E. Charney, and G. Felsenfeld, 1980, Cell, 22:87-96). These observations are discussed in the context of H1 location in the higher order chromatin fiber.
D000818:Animals; D001482:Base Composition; D002417:Cattle; D002540:Cerebral Cortex / Q000648:ultrastructure; D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA / Q000302:isolation & purification*; D008854:Microscopy, Electron; D008970:Molecular Weight; D009457:Neuroglia / Q000648:ultrastructure*; D009474:Neurons / Q000648:ultrastructure*; D009707:Nucleosomes / Q000648:ultrastructure
D002843:Chromatin; D009707:Nucleosomes; D004247:DNA