High-performance liquid chromatographic analysis of nadolol and bendroflumethiazide combination tablet formulations.
A reverse-phase high-performance liquid chromatographic (HPLC) method was developed for the simultaneous assay of nadolol and bendroflumethiazide in tablet formulations. The tablets were extracted with methanol and, after centrifugation, were chromatographed. A phenyl column was used with a mobile phase of aqueous acetate buffer with sodium chloride-methanol (60:40); detection was at 270 nm. Linearity of both drugs was satisfactory. The procedure can be automated and also applied to bendroflumethiazide formulations and bulk material.
D001539:Bendroflumethiazide / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D009248:Nadolol; D011412:Propanolamines / Q000032:analysis*; D012997:Solvents; D013607:Tablets / Q000032:analysis
D004338:Drug Combinations; D011412:Propanolamines; D012997:Solvents; D013607:Tablets; D009248:Nadolol; D001539:Bendroflumethiazide