title: Factors affecting the labeling of human fibrinogen with 99mTc in vitro.
abstract: The preparation of [99mTc]fibrinogen is described. The following factors which could affect the quality of this preparation were studied: pH, SnCl2 and protein concentrations, pre- and post-labeling incubation times and labeling temperature. A 90% labeling yield was achieved by incubating fibrinogen with SnCl2 at pH 9.8 for 22 h before adding the TcO-4 solution. The product obtained was stable and was 85% clottable. The x-ray fluorescence technique was employed to measure the amount of bound tin at different times.
mesh_terms: D005340:Fibrinogen; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007553:Isotope Labeling / Q000379:methods; D013667:Technetium; D013696:Temperature; D013997:Time Factors; D014001:Tin; D017971:Tin Compounds
chemical_list: D017971:Tin Compounds; C023599:stannous chloride; D013667:Technetium; D014001:Tin; D005340:Fibrinogen