A new approach to photometry of glycated hemoglobin in human blood.
This new approach to measurement of glycated hemoglobin (Hb A1) is a three-step procedure involving (a) conversion of oxyhemoglobin into NO-hemoglobin by sodium dithionite and sodium nitrite in the presence of inositol hexaphosphate; (b) incubation with haptoglobin, which preferentially binds glycated hemoglobin; and (c) determination of the hemoglobin/haptoglobin complex by its peroxidase activity in acid buffer. The procedure is suitable for automated analysis with instruments that are capable of adding a starting reagent. We describe preliminary adaptations for the Abbott VP and Hitachi 705 analyzers and demonstrate the correlation of results with Hb A1 concentrations determined by ion-exchange chromatography.
D001322:Autoanalysis; D006442:Glycated Hemoglobin / Q000032:analysis* / Q000378:metabolism; D006242:Haptoglobins / Q000378:metabolism; D006441:Hemoglobin A / Q000032:analysis / Q000378:metabolism; D006801:Humans; D010783:Photometry / Q000379:methods
D006442:Glycated Hemoglobin; D006242:Haptoglobins; D006441:Hemoglobin A