Effects of different forms of dialytic treatment on serum antibacterial activity in patients with chronic renal failure.
The antibacterial activity of fresh and heat-inactivated normal serum was compared with that of sera from patients with renal failure: 16 on diet, 9 on regular hemodialysis (HD) and 9 on continuous ambulatory peritoneal dialysis (CAPD). The antibacterial activity was determined on Proteus rettgeri (Sanelli) by a turbidimetric method. The inhibitory activity of fresh serum was only slightly decreased in nondialyzed uremic patients, whereas it was significantly impaired in CAPD and HD patients. Heat-inactivated normal serum (56 degrees C, 30 min) lost its antibacterial activity. Only CAPD patients' sera behaved as the normal ones. In fact, a consistent residual antibacterial activity was found in heat-inactivated sera of nondialyzed and hemodialyzed patients. The results are in keeping with the view that uremic patients have reduced host-defence reactions. Dialysis treatment appears to further depress the antibacterial capacity of uremic patient's sera. The residual antibacterial activity of heat-inactivated sera is due to substances, not present in normal controls, which act with a mechanism independent of the complement system. CAPD seems more effective than hemodialysis in removing these substances.
D000328:Adult; D000368:Aged; D001770:Blood Bactericidal Activity; D003176:Complement C3 / Q000032:analysis; D003181:Complement C4 / Q000032:analysis; D004044:Dietary Proteins / Q000008:administration & dosage; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000276:immunology / Q000628:therapy*; D008297:Male; D008875:Middle Aged; D010530:Peritoneal Dialysis; D010531:Peritoneal Dialysis, Continuous Ambulatory; D011511:Proteus / Q000276:immunology; D006435:Renal Dialysis
D003176:Complement C3; D003181:Complement C4; D004044:Dietary Proteins