Extraction of the hormonal substance from hop.
Substances that suppressed a gain in the weight of pregnant mare's serum gonadotropin (PMSG)-primed immature rat ovaries, were obtained from dried powder of the hop cone. The substances were designated F1a-I and F1a-II. In immature rats at 4 days after PMSG priming, ovarian weights decreased by 58.0 +/- 3.7 and 66.9 +/- 6.6% the control by injections with 4 mg each of F1a-I and F1a-II, respectively. Apparent M1s of the partially purified fractions were nearly 80000 (F1a-I) and 66000-80000 (F1a-II). They were water-soluble. Acidic and neutral sugars were detected in the hydrolysate of the substance.
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D005260:Female; D006064:Gonadotropins, Equine / Q000037:antagonists & inhibitors; D006728:Hormones / Q000302:isolation & purification* / Q000494:pharmacology; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012995:Solubility; D014599:Uterus / Q000187:drug effects
D006064:Gonadotropins, Equine; D006728:Hormones; D010936:Plant Extracts