[Effect of the pH of the lysing solution on the sedimentation properties of the nucleoid of intact and irradiated Ehrlich ascites tumor cells].
A study was made of the sedimentation properties of Ehrlich ascites tumor cell nucleotide obtained by cell lysis in 1.95 M NaCl, 0.1 M EDTA, 0.02 M tris, and 0.5% triton X-100, at pH 5.0 and 8.0. It was shown that the nucleotide obtained at pH 5.0 has a more compact structure than that obtained at pH 8.0. Irradiation of cells leads to relaxation of the nucleotide decreasing the rate of its sedimentation in a neutral sucrose gradient. The influence of irradiation on the sedimentation properties is more pronounced with pH 5.0 than 8.0.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis* / Q000532:radiotherapy; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis / Q000187:drug effects* / Q000528:radiation effects; D002499:Centrifugation, Density Gradient; D004273:DNA, Neoplasm / Q000032:analysis / Q000528:radiation effects; D005720:Gamma Rays; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D012996:Solutions
D004273:DNA, Neoplasm; D012996:Solutions
Vliianie pH liziruiushchego rastvora na sedimentatsionnye svoĭstva nukleoida intaktnykh i obluchennykh kletok astsitnoĭ opukholi Erlikha.