Effects of ultraviolet light on the in vitro assembly of microtubules.
Exposure of microtubular protein to ultraviolet light inhibits its assembly into morphologically normal microtubules. This effect appeared to result primarily from damage to the tubulin dimers. The damage consisted of a conformational change, a loss of two free sulfhydryl groups, a production of higher molecular weight cross-linked species, and the formation of aggregated amorphous material upon polymerization.
D000818:Animals; D001921:Brain / Q000648:ultrastructure; D002417:Cattle; D002942:Circular Dichroism; D007399:Interphase; D008854:Microscopy, Electron; D008870:Microtubules / Q000528:radiation effects*; D009391:Nephelometry and Turbidimetry; D011108:Polymers / Q000378:metabolism; D013463:Sulfuric Acid Esters / Q000378:metabolism; D014404:Tubulin / Q000528:radiation effects; D014461:Ultracentrifugation; D014466:Ultraviolet Rays
D011108:Polymers; D013463:Sulfuric Acid Esters; D014404:Tubulin; C007482:dimethyl sulfate