Electrofusion. A new, highly efficient technique for generating somatic cell hybrids.
A rapid and highly efficient method for generating viable somatic cell hybrids is described. A co-culture of clone ID and CH rodent cell lines was exposed to five successive electric pulses of about 1.5 kV/cm with a duration of 50 musec. This treatment induced extensive cell fusion, and independent hybrid clones were generated with a frequency of 1 X 10(-3), which represents a 100-fold increase over the polyethylene glycol induced fusion. Seventeen of them were propagated in selective medium, karyotyped and analysed for their enzyme markers, in order to establish their hybrid nature.
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D006224:Cricetinae; D004560:Electricity; D006822:Hybrid Cells / Q000166:cytology* / Q000201:enzymology; D007527:Isoenzymes / Q000032:analysis; D007621:Karyotyping; D008722:Methods; D051379:Mice
D007527:Isoenzymes