Direct analysis for urinary protein with biuret reagent, with use of urine ultrafiltrate blanking: comparison with a manual biuret method involving trichloroacetic acid precipitation.
We describe a method for measuring urinary protein with a centrifugal analyzer. Biuret reagent is used, and blanking with an ultrafiltrate of urine eliminates interferences from the nonprotein, biuret-positive chromogens in urine. We compare results by this new method with those by a manual method in which trichloroacetic acid precipitation and biuret reagent are used. The new method shows good precision and excellent correlation (r = 0.997) with the manual method. The ease and convenience of this assay should make this a useful method for the routine clinical laboratory.
D000900:Anti-Bacterial Agents / Q000652:urine; D001738:Biuret Reaction; D011232:Chemical Precipitation; D003124:Colorimetry / Q000379:methods*; D005189:False Positive Reactions; D006801:Humans; D011506:Proteins / Q000032:analysis*; D011507:Proteinuria / Q000652:urine*; D014238:Trichloroacetic Acid; D014462:Ultrafiltration
D000900:Anti-Bacterial Agents; D011506:Proteins; D014238:Trichloroacetic Acid