Vitamin E, catalase, manganous or cobaltous ions and dithiothreitol protect against Tween 20-induced hemolysis of vitamin E-deficient chick and kid erythrocytes.
The effective Tween 20 concentration at which 70% hemolysis was achieved in vitro correlated with the plasma vitamin E content of chicks (r = 0.85). Addition of catalase, MnCl2, CoCl2 or dithiothreitol in vitro showed significant protection against the hemolysis induced by Tween 20 in vitamin E-deficient chick and kid erythrocytes.
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002645:Chickens; D003035:Cobalt / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D006041:Goats; D006461:Hemolysis / Q000187:drug effects*; D008297:Male; D008345:Manganese / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology; D014811:Vitamin E Deficiency / Q000097:blood*
D011136:Polysorbates; D014810:Vitamin E; D003035:Cobalt; D008345:Manganese; D002374:Catalase; D004229:Dithiothreitol