Hemoprotein catalysis of leukotriene formation.
Incubation of various hemoproteins with 5-hydroperoxy-6,8,11,14-eicosatetraenoic acid or 15-hydroperoxy-5,8,11,13-eicosatetraenoic acid resulted in formation of epimeric 5(S),12-dihydroxy-6,8,10,14 -eicosatetraenoic acids and epimeric 8,15(S)-dihydroxy-5,9,11,13 -eicosatetraenoic acids, respectively. These dihydroxy acids were earlier recognized as nonenzymatic hydrolysis products of 5(S),6-oxido-7,9,11,14-eicosatetraenoic acid (leukotriene A4) and 14,15(S)-oxido-5,8,10,12-eicosatetraenoic acid (14,15-leukotriene A4). These allylic epoxides could be isolated as such from the hemoprotein incubations, and most probably they are intermediates in formation of the dihydroxy acids.
D001095:Arachidonic Acids / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003580:Cytochromes / Q000494:pharmacology*; D005296:Ferrous Compounds / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D006735:Horseradish Peroxidase / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology*; D015289:Leukotrienes; D008054:Lipid Peroxides / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism*; D013189:SRS-A / Q000378:metabolism*
D001095:Arachidonic Acids; D003580:Cytochromes; D005296:Ferrous Compounds; D015289:Leukotrienes; D008054:Lipid Peroxides; D013189:SRS-A; C025086:15-hydroperoxy-5,8,11,13-eicosatetraenoic acid; C025348:arachidonic acid 5-hydroperoxide; D007501:Iron; D006735:Horseradish Peroxidase; D010544:Peroxidases