Fatty acid oxidation by developing rat kidney.
In view of the importance of free fatty acids (FFA) as substrates for the mature kidney, fatty acid oxidation by developing rat kidney has been investigated in vitro. Incubations of kidney slices from fetal stages (days 20 and 21 of gestation) and from days 1, 5, 10, 20, and 30 after birth have been carried out in Krebs-phosphate, pH 7.4, containing 0.5 or 1.5 mM U-14C-palmitate. Palmitate uptake and oxidation into CO2 were measured after 90 min incubation. At 0.5 mM concentration of palmitate in the incubation medium, fetal kidney exhibited a low uptake and a very low oxidation of palmitate into CO2. These two parameters increased only after birth and were maximum with slices from suckling rats between days 5 and 10 after birth. Palmitate oxidation increased only with postnatal days slices (maximum during the suckling period) and not with fetal slices. An increase in palmitate oxidation could be obtained with slices from kidneys of fetuses whose mothers had been starved 48h. These data suggest that the development of renal capacity for FFA oxidation during the perinatal period could be related to nutritional supply.
D000818:Animals; D002149:Energy Intake; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D005260:Female; D005508:Food Deprivation; D005865:Gestational Age; D007668:Kidney / Q000196:embryology* / Q000378:metabolism; D010168:Palmitates / Q000378:metabolism; D011247:Pregnancy; D051381:Rats
D005230:Fatty Acids, Nonesterified; D010168:Palmitates