High-performance liquid chromatographic method for post-column, in-line derivatization with o-phthalaldehyde and fluorometric detection of phenylpropanolamine in human urine.
A rapid and sensitive method for the analysis of phenylpropanolamine in urine was developed using high-pressure liquid chromatography, post-column, in-line derivatization with o-phthalaldehyde, followed by detection with a fluorometer. Human urine was injected directly into the chromatographic system, and phenylpropanolamine separated in the reversed-phase mode. The practical lower limit of detection was 0.1 microgram/ml of urine. The coefficient of variation from 0.96 to 96 micrograms/ml varied between 2.23 and 0.19%, respectively. The linearity of the calibration graphs is excellent (r = 0.9999) over a concentration range of two orders of magnitude.
D000661:Amphetamine / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D010665:Phenylpropanolamine / Q000652:urine*; D013050:Spectrometry, Fluorescence / Q000379:methods; D009764:o-Phthalaldehyde
D010665:Phenylpropanolamine; D009764:o-Phthalaldehyde; D000661:Amphetamine