Detection of a yeast polyphosphate fraction localized outside the plasma membrane by the method of phosphorus-31 nuclear magnetic resonance.
Non-penetrating cations, like UO2+(2) and Eu3+, are bound to the outside of yeast cells in a reversible fashion. Binding of these ions was attended with a decrease of the 31P NMR polyphosphate signal. Subsequent addition of EDTA to the suspension restored the original spectrum. These experiments confirm the localization of a polyphosphate fraction outside the plasma membrane of yeast.
D002462:Cell Membrane / Q000032:analysis; D005063:Europium / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D011122:Polyphosphates / Q000032:analysis* / Q000378:metabolism; D012440:Saccharomyces / Q000032:analysis* / Q000378:metabolism; D014501:Uranium / Q000378:metabolism; D017974:Uranium Compounds
D011122:Polyphosphates; D017974:Uranium Compounds; D005063:Europium; D014501:Uranium; C012597:uranium dioxide