Human and porcine immunoreactive gastric inhibitory polypeptides (IR-GIP) are not identical.
Immunoreactive gastric inhibitory polypeptide (IR-GIP) from human and porcine intestine was quantified by radioimmunoassay and the molecular forms characterised by gel permeation and reverse-phase high pressure liquid chromatography (HPLC). Gel filtration revealed two major immunoreactive peaks corresponding to the previously described 5-kDa and 8-kDa molecular forms, which appeared similar in both species. Isocratic reverse-phase HPLC revealed that the major immunoreactive GIP peak (5-kDa) in the human tissue eluted earlier than the corresponding porcine molecular form, indicating the latter to be less hydrophobic. These findings suggest significant species differences between human and porcine GIP.
D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004386:Duodenum / Q000032:analysis; D005749:Gastric Inhibitory Polypeptide / Q000302:isolation & purification*; D005768:Gastrointestinal Hormones / Q000302:isolation & purification*; D006801:Humans; D007413:Intestinal Mucosa / Q000032:analysis; D011863:Radioimmunoassay; D013045:Species Specificity; D013552:Swine
D005768:Gastrointestinal Hormones; D005749:Gastric Inhibitory Polypeptide