title: Effect of type A Pasteurella multocida fractions on bovine polymorphonuclear leukocyte functions.
abstract: The effect of various Pasteurella multocida fractions on bovine polymorphonuclear leukocyte (PMN) functions was examined in vitro by using two encapsulated strains, P-2383 and P-1062 (both are Carter capsular type A and of bovine origin). The ability of PMNs to ingest Staphylococcus aureus and iodinate protein was significantly inhibited in the presence of live cells, heat-killed whole cells, or saline-extracted capsules but not in the presence of the decapsulated heat-killed cells. None of the fractions of the two strains inhibited nitroblue tetrazolium reduction by PMNs. The saline extract did not inhibit the binding of iodine to protein by a reaction involving xanthine, xanthine oxidase, and horseradish peroxidase. The PMN inhibitory factor was further characterized as a heat-stable capsular material of greater than 300,000 molecular weight.
mesh_terms: D000818:Animals; D002417:Cattle; D006820:Hyaluronic Acid / Q000032:analysis; D009504:Neutrophils / Q000502:physiology*; D009580:Nitroblue Tetrazolium; D010325:Pasteurella / Q000472:pathogenicity*; D010587:Phagocytosis; D013045:Species Specificity; D013211:Staphylococcus aureus
chemical_list: D009580:Nitroblue Tetrazolium; D006820:Hyaluronic Acid