Detection of specific IgE antibodies in sera from patients with hydatidosis.
A simple and sensitive immunoenzymatic assay for detection of specific IgE in hydatid disease is presented. Sera from 43 patients with hydatidosis, eight with schistosomiasis, two with taeniasis, two with cancer and 16 healthy blood donors were tested by both the immunoenzymatic technique and the classical radioallergosorbent test. Data were analysed in terms of different sensitivity and specificity of the two methods. The results indicate that the enzymatic method performed in microplates is as sensitive as the radioimmunological one (77% of positivity for both the tests), but the latter suffers from an higher rate of false positive reactions with sera from patients with other helminthic diseases (80% vs 20%). When the specific IgE evaluation is compared with other classical serological techniques, such as indirect haemagglutination and double diffusion in terms of different sensitivity in revealing an anti-Echinococcus immunological reactivity, the results suggest that specific IgE evaluation represents a useful addition to the classical serodiagnostic tests.
D004443:Echinococcosis / Q000175:diagnosis / Q000276:immunology*; D004446:Echinococcus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*; D009369:Neoplasms / Q000276:immunology; D011852:Radioallergosorbent Test; D012552:Schistosomiasis / Q000276:immunology; D013622:Taeniasis / Q000276:immunology
D007073:Immunoglobulin E