title: High-performance liquid chromatography with polarographic and voltammetric anodic detection: simultaneous determination of allopurinol, oxipurinol and uric acid in body fluids.
abstract: Allopurinol, oxipurinol and uric acid have been determined in human serum and urine by liquid chromatography with electrochemical detection. In particular the use of a polarographic detector operating in the oxidative mode, whose principle of detection is based on the property of allopurinol, oxipurinol and uric acid to form insoluble anodic films on mercury, is described. The performance of such a detector is compared with that of a glassy carbon wall-jet detector. Different procedures for sample pretreatment have been evaluated.
mesh_terms: D000493:Allopurinol / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D006801:Humans; D010117:Oxypurinol / Q000032:analysis* / Q000097:blood / Q000652:urine; D011048:Polarography / Q000379:methods; D011743:Pyrimidines / Q000032:analysis*; D014527:Uric Acid / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D011743:Pyrimidines; D014527:Uric Acid; D000493:Allopurinol; D010117:Oxypurinol