Spindle-membrane associations in freeze fractured HeLa cells.
Mitotic HeLa cells, obtained by means of thymidine and nocodazole synchronization, were freeze fractured, and spindle-membrane associations were studied. Except for small vesicles most membrane structures were excluded from the spindle region. A few tubular to lamellar membrane arrays were observed that projected into the spindle region from the vicinity of the spindle poles. Concentric layers of flattened, extensively fenestrated cisternae were found in the cytoplasm surrounding the spindle. The plasma membrane overlying the midbody in early G1 cells was examined.
D005614:Freeze Fracturing; D006367:HeLa Cells; D006801:Humans; D007425:Intracellular Membranes / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008938:Mitosis; D008941:Spindle Apparatus / Q000648:ultrastructure*