Occupational asthma from nickel sensitivity: II. Factors influencing the interaction of Ni2+, HSA, and serum antibodies with nickel related specificity.
Serum from an individual with occupational asthma from nickel sulphate inhalation was shown by ammonium sulphate (Farr) and anti-IgG coprecipitation tests to contain antibodies which bind 63Ni2+. This binding was absent from 30 control sera. Ligand competition studies with serum and plasma from the propositus and controls are described, and include serial additions of HSA, L-histidine, EDTA, and the tripeptide gly-gly-L-his. The results, like those from metal ion blocking experiments, clearly showed that the formation of the reactive antigenic determinant depends on the selective binding of Ni2+ at the native Cu2+/Ni2+ transport site of HSA. Corroborating evidence is derived from the similar dependence on pH of the Ni2+-HSA formation and the ammonium sulphate 63Ni2+ coprecipitation reactions.
D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001249:Asthma / Q000139:chemically induced / Q000276:immunology*; D001667:Binding, Competitive; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008670:Metals / Q000276:immunology; D009532:Nickel / Q000009:adverse effects* / Q000276:immunology*; D009784:Occupational Diseases / Q000139:chemically induced / Q000276:immunology*; D011233:Precipitin Tests; D012709:Serum Albumin / Q000276:immunology*
D000906:Antibodies; D007074:Immunoglobulin G; D008670:Metals; D012709:Serum Albumin; C029938:nickel sulfate; D009532:Nickel