In vitro colony formation by cryopreserved primary human tumor cells.
The effect of cryopreservation on the ability of primary human cancer cells to form colonies in a two-layer agar system was examined. Although considerable variation occurred, concentrations of 5 or 10% dimethylsulfoxide employed with slow freezing rates allowed survival of colonies in the range 20-40% or greater of nonfrozen controls. The methods used in this study do not require elaborate freezing equipment, and can be used for the cryopreservation of a wide variety of types of cancers.
D003114:Colony-Forming Units Assay; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide; D005615:Freezing; D005990:Glycerol; D006801:Humans; D009369:Neoplasms / Q000473:pathology*; D011309:Preservation, Biological; D014410:Tumor Stem Cell Assay
D003451:Cryoprotective Agents; D005990:Glycerol; D004121:Dimethyl Sulfoxide