Neurite growth cone-substratum adherence increases in vitro.
During experiments characterizing the turning response of dorsal root ganglion neurites toward NGF, it was observed that growth cone-substratum adherance increased with time in culture. The experiments reported here indicate that the observed increase in growth cone-substratum adherance is significant and can be detected with both collagen and poly-L-lysine substrates. The increased adherance is apparently due to a substance(s) produced and released by the ganglia which binds to the substrate, increasing adherance. Flow chamber studies indicate that the substrate-bound substance(s) may be necessary for neurite growth onto artificial tissue culture substrata.
D000818:Animals; D002448:Cell Adhesion; D002642:Chick Embryo; D003094:Collagen; D005727:Ganglia, Spinal / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009924:Organ Culture Techniques; D011107:Polylysine
D009414:Nerve Growth Factors; D011107:Polylysine; D003094:Collagen