[Physicochemical properties of kidney-specific antigen].
Using specific antirenal sera obtained from rabbits and absorbed with a mixture of extracts from heterologous organs, a specific antigen was detected in human and CBA mouse renal extracts. Its molecular weight was found to amount to about 100 000 dalton. It is salted out with ammonium sulfate at 50-70% saturation of renal extract and is destroyed on extract heating for 30 min at 75 degrees C. This antigen is sensitive to trypsin and papain but resistant to hyaluronidase. It is partially destroyed by DNase and RNase, provided the latter ones are used in comparatively high doses (1 mg per 0.3 ml extract) and exposure lasts one day. Based on the study of the physicochemical properties it is suggested that the kidney-specific antigen may be a ribonucleoprotein or a deoxyribonucleoprotein but cannot be attributed to glycoproteins.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification*; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D009928:Organ Specificity
D000941:Antigens
Izuchenie fiziko-khimicheskikh svoĭstv pochechno-spetsificheskogo antigena.