The absorption and transport of dietary cholesterol in the presence of peanut oil or randomized peanut oil.
Peanut oil has been shown to be unexpectedly atherogenic for cholesterol-fed rats, rabbits and rhesus monkeys. However, randomization (rearrangement of fatty acids to random distribution) of peanut oil significantly reduced its atherogenicity for rabbits and monkeys. This study was conducted to investigate whether the absorption and transport of dietary cholesterol was altered in the presence of peanut oil or randomized peanut oil, thereby accounting for the difference in the atherogenicity of the two diets. Intestinal lymph fistula rats were infused intraduodenally with a lipid emulsion at a rate of 3 ml/hr. The lipid emulsion contained either peanut oil (control) or randomized peanut oil (experimental) (10 mM), 14C-cholesterol (1.3 mM) and sodium taurocholate (19 mM) in phosphate-buffered saline, pH 6.4. Lymph triglyceride, cholesterol and phospholipid outputs were similar in both groups of rats during fasting and subsequently during lipid infusion. Comparable recovery of 14C-cholesterol from the intestinal lumen and the intestinal mucosa of the control and the experimental rats showed that the absorption and transport of dietary cholesterol were similar in both groups of rats. Analyses of the fatty acid of both lymph and intestinal mucosal lipid again failed to reveal a difference between the 2 groups of rats. It is concluded that the difference in the atherogenicity between the peanut oil and the randomized peanut oil is probably caused by events subsequent to the release of cholesterol containing chylomicrons and very low density lipoproteins by the small intestinal epithelial cells.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002791:Cholesterol, Dietary / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D007408:Intestinal Absorption / Q000187:drug effects*; D008196:Lymph / Q000378:metabolism; D008297:Male; D008780:Methyltransferases / Q000378:metabolism*; D009821:Oils / Q000494:pharmacology*; D000074241:Peanut Oil; D050941:Phosphatidyl-N-Methylethanolamine N-Methyltransferase; D010743:Phospholipids / Q000378:metabolism; D010938:Plant Oils; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000378:metabolism
D002791:Cholesterol, Dietary; D005227:Fatty Acids; D009821:Oils; D000074241:Peanut Oil; D010743:Phospholipids; D010938:Plant Oils; D014280:Triglycerides; D008780:Methyltransferases; D050941:Phosphatidyl-N-Methylethanolamine N-Methyltransferase