title: Biochemical studies on the use of sodium hyaluronate in the anterior eye segment. II. The molecular behavior of sodium hyaluronate injected into anterior chamber of rabbits.
abstract: The behavior of sodium hyaluronate injected into the anterior chamber of rabbits was investigated using a 1% (w/v) solution of sodium hyaluronate having a molecular weight of 800K. Although the aqueous humor contained a considerable amount of reduced ascorbic acid, the molecular weight of sodium hyaluronate injected into the anterior chamber did not change. This was due to existence of a protecting factor for degradation of sodium hyaluronate in a low molecular weight fraction of the aqueous humor. The injected sodium hyaluronate was found to aggregate with proteins in the aqueous humor within 2 hrs. However, this aggregation did not affect the degradation of sodium hyaluronate with ascorbic acid. The hyaluronic acid content in the iris-ciliary body increased about three times as much as the normal level 2 hrs after the sodium hyaluronate injection. This result suggests that the injected sodium hyaluronate is not only eliminated through the angular aqueous plexus but also through the iris-ciliary body, followed by digestion with hyaluronidase in those tissues.
mesh_terms: D000818:Animals; D000867:Anterior Chamber; D001082:Aqueous Humor / Q000032:analysis / Q000378:metabolism*; D001205:Ascorbic Acid / Q000032:analysis; D002924:Ciliary Body / Q000378:metabolism; D005123:Eye / Q000378:metabolism*; D005260:Female; D006820:Hyaluronic Acid / Q000378:metabolism* / Q000627:therapeutic use; D007267:Injections; D007498:Iris / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D013508:Ophthalmologic Surgical Procedures; D011817:Rabbits
chemical_list: D006820:Hyaluronic Acid; D001205:Ascorbic Acid