title: Effects of ultraviolet light on the in vitro assembly of microtubules.
abstract: Exposure of microtubular protein to ultraviolet light inhibits its assembly into morphologically normal microtubules. This effect appeared to result primarily from damage to the tubulin dimers. The damage consisted of a conformational change, a loss of two free sulfhydryl groups, a production of higher molecular weight cross-linked species, and the formation of aggregated amorphous material upon polymerization.
mesh_terms: D000818:Animals; D001921:Brain / Q000648:ultrastructure; D002417:Cattle; D002942:Circular Dichroism; D007399:Interphase; D008854:Microscopy, Electron; D008870:Microtubules / Q000528:radiation effects*; D009391:Nephelometry and Turbidimetry; D011108:Polymers / Q000378:metabolism; D013463:Sulfuric Acid Esters / Q000378:metabolism; D014404:Tubulin / Q000528:radiation effects; D014461:Ultracentrifugation; D014466:Ultraviolet Rays
chemical_list: D011108:Polymers; D013463:Sulfuric Acid Esters; D014404:Tubulin; C007482:dimethyl sulfate