title: Comparison of 1-acylglycerophosphate and glycerophosphate acyltransferases from Euglena microsomes.
abstract: Both glycerophosphate and monoacylglycerophosphate acyltransferases from Euglena microsomes were inhibited by N-ethylmaleimide, but their responses to heat inactivation and sn-glyceraldehyde-3-phosphate differed. Glycerophosphate acyltransferase had a higher V with palmitoyl-CoA compared to oleoyl-CoA; the reverse was true for monoacylglycerophosphate acyltransferase. Km's (microM) for the glycerophosphate acyltransferase were: palmitoyl-CoA, 21; oleoyl-CoA, 14; and sn-glycerol-3-phosphate, 2900. Km's (microM) for monoacylglycerophosphate acyltransferase were: palmitoyl-CoA, 7; oleoyl-CoA, 4; and 1-palmitoyl-sn-glycerol-3-phosphate, 48.
mesh_terms: D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase; D000217:Acyltransferases / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D002384:Catalysis; D005055:Euglena / Q000201:enzymology*; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D006358:Hot Temperature; D008861:Microsomes / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D000217:Acyltransferases; D005992:Glycerol-3-Phosphate O-Acyltransferase; D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase