Thermotropic change in phospholipid packing in microsomal membranes sensed by phospholipase A2.
A reversible, temperature-dependent change in phospholipid packing occurring between 0 degree C and 12 degrees C has been identified in microsomal membranes by the use of phospholipase A2 from Crotalus atrox. It manifests itself as a drastic increase in susceptibility to the phospholipase and depends on non-lipid (presumably protein) membrane components. It is suggested that this change could underlie the change in transmembrane mobility of phospholipids which occurs in the same temperature range.
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003435:Crotalid Venoms / Q000032:analysis; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013552:Swine; D013816:Thermodynamics
D003435:Crotalid Venoms; D008563:Membrane Lipids; D010743:Phospholipids; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2