Effects of D-penicillamine on growth and cell cycle kinetics of cultured rabbit articular chondrocytes.
The long-acting antirheumatic drug D-penicillamine was found to inhibit the growth of asynchronous cultures of rabbit articular chondrocytes. This inhibitory effect was dose-related between 5 X 10(-4) M and 5 X 10(-3) M and was time-dependent for a given dose. Flow cytometric analysis showed that drug exposure led to a slowdown in cell cycle progression. This was manifested as a decrease in the number of cells in S phase, due especially to an accumulation of cells in G0 G1 and also to a slight cessation of cell transit through G2 M. Recovery experiments showed that the effect is transitory and reversible. It is suggested that the articular chondrocyte is a target cell for D-penicillamine and that these cells have a D-penicillamine sensitive restriction point in the G0 G1 phase of the cell cycle and to a less extent in the G2 M phase.
D000818:Animals; D002358:Cartilage, Articular / Q000032:analysis / Q000166:cytology / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002999:Clone Cells / Q000187:drug effects; D004247:DNA / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D010396:Penicillamine / Q000494:pharmacology*; D011817:Rabbits
D004247:DNA; D010396:Penicillamine