Composition of the peptidoglycan of alkalophilic Bacillus spp.
Peptidoglycans of 10 alkalophilic Bacillus strains were isolated as trichloroacetic acid-insoluble materials from cell walls prepared by treatment with sodium dodecyl sulfate, disruption with a sonic oscillator, and trypsin digestion. Major constituents detected commonly in hydrolysates of the peptidoglycans were glucosamine, muramic acid, D- and L-alanine, D-glutamic acid, meso-diaminopimelic acid, and acetic acid. Ammonia derived from amide was found in a portion of the hydrolysates. The composition of peptidoglycan was not changed whether the strain was cultured at pH 7 or 10. All the peptidoglycan examined was of the A1 gamma type of peptidoglycan found in most strains of the genus Bacillus.
D000596:Amino Acids / Q000032:analysis; D001407:Bacillus / Q000254:growth & development*; D005944:Glucosamine / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D009112:Muramic Acids / Q000032:analysis; D010457:Peptidoglycan / Q000032:analysis*; D013045:Species Specificity
D000596:Amino Acids; D009112:Muramic Acids; D010457:Peptidoglycan; D005944:Glucosamine