Stereochemical course of phospho group transfer by human prostatic acid phosphatase.
The stereochemical course of the phospho transfer catalyzed by homogeneous human prostatic acid phosphatase was investigated using 31P nuclear magnetic resonance spectroscopy. Transphosphorylation from phenyl-(R)-[15O, 17O, 18O]phosphate to (S)-propane-1,2-diol occurs with overall retention of configuration at phosphorus. This stereochemical result is consistent with the interpretation that the hydrolysis of substrates by this enzyme proceeds by way of a covalent phosphoenzyme intermediate. Conditions for optimizing phospho transfer by this and related acid phosphatases have also been explored.
D000135:Acid Phosphatase / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010103:Oxygen Isotopes; D010766:Phosphorylation; D011467:Prostate / Q000201:enzymology*; D011485:Protein Binding; D013237:Stereoisomerism
D010103:Oxygen Isotopes; D000135:Acid Phosphatase