The effect of phenobarbital on protein metabolism of liver. Results with isolated hepatocytes.
A technique has been devised which makes use of an amino acid alternatively labeled with either [14C] or [3H], and permits the simultaneous evaluation of synthesis, catabolism and secretion by the same sample of isolated rat liver cells during the same time-period of incubation. This technique has been used to study protein metabolism of liver cells isolated from rats treated with 4 doses of phenobarbital (8 mg/100 g body weight) given in the 4 days before killing the animals. Total protein synthesis and secretion do not change in phenobarbital-treated rats; albumin represents 40% of secreted protein in both normal and treated rats. On the contrary, the parameters which indicate protein degradation are lower in phenobarbital-treated than in normal rats, showing that protein catabolism is appreciably reduced in the liver cells obtained from rats treated with phenobarbital.
D000418:Albumins / Q000378:metabolism; D000818:Animals; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008722:Methods; D010634:Phenobarbital / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000418:Albumins; D011506:Proteins; D010634:Phenobarbital