Experimental investigations on the influence of stroma-free haemoglobin solution on kidney function in exchange transfusion models.
In the continuing search for promoting the bio-pharmacological characteristics of an excellent oxygen carrier, the Stroma-free Haemoglobin Solution (SFHS), in the presented work, the authors were investigating the composition changes of SFHS infused to dogs. In 10 mongrel dogs a total exchange of the circulating blood was done for replacing it with SFHS. For the observing the physico-chemical changes in circulating and excreted fluids electron spectroscopy was used in view of good spectral characteristics of haemoglobin, and a high sensitivity of this method to changes taking place in the analyzed molecule. Comparing the spectral curves characterizing the solution used for exchange transfusion with the spectral curves obtained from circulating SFHS samples and haemoglobin excreted in the urine, and from the ascites, during the 6 hours course of the experiment, no essential structural changes were observed in the hemin molecule, in the visible range.
D000818:Animals; D004285:Dogs; D005078:Exchange Transfusion, Whole Blood; D006454:Hemoglobins / Q000494:pharmacology*; D006456:Hemoglobinuria / Q000652:urine; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000187:drug effects / Q000502:physiology*; D011188:Potassium / Q000652:urine; D012964:Sodium / Q000652:urine; D013056:Spectrophotometry, Ultraviolet
D006454:Hemoglobins; C016484:hemoglobin, stroma free; D012964:Sodium; D011188:Potassium