Short-term regulation of muscarinic acetylcholine receptor binding in cultured nerve cells.
Short-term preincubation of mouse neuroblastoma cells (clone N1E-115) with the muscarinic agonist carbamylcholine at 37 degrees C resulted in a significant decrease in the binding of the muscarinic antagonist [3H]N-methylscopolamine. This agonist-induced response reached a steady state at 30 min. It was also dose dependent, saturable and sensitive to temperature, suggesting a receptor-mediated phenomenon. The significance of these findings is discussed.
D000818:Animals; D002217:Carbachol / Q000378:metabolism; D002999:Clone Cells; D051379:Mice; D019832:N-Methylscopolamine; D009447:Neuroblastoma / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000378:metabolism*; D012602:Scopolamine Derivatives
D011976:Receptors, Muscarinic; D012602:Scopolamine Derivatives; D002217:Carbachol; D019832:N-Methylscopolamine