Long-chain Acyl-CoA levels in liver from rats fed high-fat diets: is it of significance for an increased peroxisomal beta-oxidation?
The levels of long-chain acyl-CoA in the livers of rats given diets containing various amounts of dietary oils were investigated. Increasing the amount of soybean oil in the diet from 5% to 25% (w/w) led to a 40% increase in long-chain acyl-CoA. With partially hydrogenated marine oil, a sigmoidal dose-response curve was obtained, giving a 60% increase when 20% or more of this oil was in the diet. All high-fat diets tested resulted in higher levels of long-chain acyl-CoA than the low-fat control containing soybean oil. The increase was most prominent with partially hydrogenated marine and rapeseed oils. With diets containing partially hydrogenated marine oil, the ratio of long-chain acyl-CoA to acid-soluble CoA was increased after 3 days, but decreased after 3 weeks, to a value similar to that observed in animals fed soybean oil because of an extensive increase in acid-soluble CoA. Increased levels of long-chain acyl-CoA were also observed after clofibrate was administered, but the increase was less prominent than observed with high-fat diets. When comparing the levels of long-chain acyl-CoA observed after 3 days on different diets with the peroxisomal beta-oxidation activity previously determined after 3 weeks on the corresponding diets, a straight line was obtained. These results are discussed in relation to the possibility that long-chain acyl-CoA induces peroxisomal beta-oxidation activity.
D000214:Acyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D004041:Dietary Fats / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008830:Microbodies / Q000187:drug effects / Q000378:metabolism*; D009821:Oils / Q000494:pharmacology; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains
D000214:Acyl Coenzyme A; D004041:Dietary Fats; D009821:Oils