Metabolism of vinylcyclooctane and partition ratio between epoxide formation and cytochrome P-450 destruction.
Vinylcyclooctane (VCO), which binds to the active site of cytochrome P-450 (P-450) giving a type I difference spectrum, has been found to form the corresponding epoxide as the main metabolite on treatment with liver microsomal monooxygenase obtained from phenobarbital-treated or untreated mice. During this metabolic process about 40% of the microsomal P-450 isozymes are destroyed, but the remainder still demethylates aminopyrine. Approx. 180 molecules of VCO are turned over and 132 of epoxyethylcyclooctane (EECO) are formed for each destructive event.
D000818:Animals; D001665:Binding Sites; D002849:Chromatography, Gas; D034242:Cyclooctanes; D003516:Cycloparaffins / Q000378:metabolism* / Q000494:pharmacology; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*
D034242:Cyclooctanes; D003516:Cycloparaffins; D065607:Cytochrome P-450 Enzyme Inhibitors; C040532:epoxyethylcyclooctane; C038434:vinylcyclooctane; D003577:Cytochrome P-450 Enzyme System