[Structural changes in erythrocyte ghosts after irradiation and the initiation of lipid oxidation detectable using 2,6-TNS and fluorescamine].
It was shown on erythrocyte ghosts that the parameters of fluorescence of 2,6-toluidine-naphthalene-sulfonate (2,6-TNS) and fluorescamine undergo similar changes after irradiation. After a dose of 100 Gy the equally effective concentrations of Fe2+ were 1-5 microM and 50-100 microM with regard to changes in the rate of fluorescence of fluorescamine and 2,6-TNS, respectively, and greater than 100 microM with regard to fluorescence anisotropy.
D000818:Animals; D004307:Dose-Response Relationship, Radiation; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000528:radiation effects*; D005296:Ferrous Compounds / Q000494:pharmacology; D005450:Fluorescamine / Q000494:pharmacology*; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes / Q000494:pharmacology*; D005720:Gamma Rays; D008054:Lipid Peroxides / Q000097:blood; D008055:Lipids / Q000097:blood*; D009282:Naphthalenesulfonates / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects / Q000528:radiation effects; D051381:Rats; D013141:Spiro Compounds / Q000494:pharmacology*
D005296:Ferrous Compounds; D005456:Fluorescent Dyes; D008054:Lipid Peroxides; D008055:Lipids; D009282:Naphthalenesulfonates; D013141:Spiro Compounds; D005450:Fluorescamine; C020748:ferrous sulfate; C009497:2-(4-toluidino)-6-naphthalenesulfonic acid
Strukturnye izmeneniia v teniakh éritrotsitov posle oblucheniia i initsiatsii okisleniia lipidov, obnaruzhivaemye s pomoshch'iu 2,6-TNS i fluoreskamina.