Defective ultraviolet-induced DNA repair in bone marrow cells and peripheral lymphocytes of patients with refractory anemia with excess of blasts.
The extent of ultraviolet (UV) irradiation-induced DNA repair was measured in bone marrow cells and peripheral lymphocytes of patients with refractory anemia with excess of blasts (RAEB). Bone marrow cells from RAEB, when exposed to a 2 J/m dose of UV, exhibited 50% lower incorporation of tritiated thymidine than those of control subjects. A similar finding was observed in the peripheral lymphocytes. These data suggest that bone marrow cells and peripheral lymphocytes from RAEB are deficient in repair of UV-induced lesions by DNA. Furthermore, this impaired DNA repair efficiency in RAEB was not related to the presence or absence of a karyotype abnormality.
D000328:Adult; D000368:Aged; D000741:Anemia, Aplastic / Q000378:metabolism*; D001853:Bone Marrow / Q000378:metabolism* / Q000528:radiation effects; D002455:Cell Division; D002478:Cells, Cultured; D004260:DNA Repair / Q000528:radiation effects*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000528:radiation effects; D008875:Middle Aged; D014466:Ultraviolet Rays