Enzymatic repair of premutagenic DNA lesions in human epidermis. Quantitation of O6-methylguanine-DNA methyltransferase and uracil-DNA glycosylase activities.
Extracts of human epidermis prepared by the suction blister method were used to measure O6-methylguanine-DNA methyltransferase and uracil-DNA glycosylase activities. Although both activities were detected in all extracts examined, a 4-5-fold interindividual variation in activity was found. No obvious correlation of the two enzyme activities with the age of the patient was observed. Neither was there any correlation between the level of uracil-DNA glycosylase activity and O6-methylguanine-DNA methyltransferase activity.
D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D045647:DNA Glycosylases; D004260:DNA Repair; D005260:Female; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D008297:Male; D008770:Methylnitrosourea / Q000378:metabolism; D008780:Methyltransferases / Q000378:metabolism*; D008875:Middle Aged; D009153:Mutagens / Q000633:toxicity*; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D019853:O(6)-Methylguanine-DNA Methyltransferase; D012016:Reference Values; D012867:Skin / Q000201:enzymology*; D012871:Skin Diseases / Q000201:enzymology*; D014316:Tritium; D051981:Uracil-DNA Glycosidase
D009153:Mutagens; D014316:Tritium; D008770:Methylnitrosourea; D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase