[Use of a standard radioimmunological analysis system for determining prolactin in the blood plasma of swine, sheep and bulls].
The system has been developed for radioimmunologic determination of the blood prolactin content in domestic animals--pig, sheep and bull, using common antiserum for the pig prolactin. This antiserum has a high antibody titer and can bind with large affinity not only the pig but also sheep and bull prolactins. Incubation system contains a final volume of 300 microliter of antiserum (1:192 000), 125I5-labelled pig hormone (30 000 imp/min) and standard or dofining hormone (0.5 to 100 micrograms/ml). To separate the free- and antibody-bound hormone fractions the double antibody method was used.
D000818:Animals; D002417:Cattle; D007106:Immune Sera / Q000302:isolation & purification; D007114:Immunization; D007457:Iodine Radioisotopes; D008297:Male; D011388:Prolactin / Q000097:blood* / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*; D012756:Sheep; D013045:Species Specificity; D013552:Swine
D007106:Immune Sera; D007457:Iodine Radioisotopes; D011388:Prolactin
Ispol'zovanie edinoĭ sistemy radioimmunologicheskogo analiza dlia opredeleniia prolaktina v plazme krovi svineĭ, ovets i bykov.