Aldosterone biosynthesis by a reconstituted cytochrome P-45011 beta system.
[3H]Corticosterone was incubated with cytochrome P-45011 beta purified to electrophoretic homogeneity from bovine adrenocortical mitochondria, and the reaction products were analyzed by high performance liquid chromatography. The production of aldosterone (21.2 pmol/nmol P-450/min) and 18-hydroxycorticosterone (1.17 nmol/nmol P-450/min) was observed. When lipidic extracts from mitochondria of bovine adrenocortical zona glomerulosa were added to the reaction mixture, the rate of production of aldosterone was increased 28-fold. When [3H]18-hydroxycorticosterone was incubated with cytochrome P-45011 beta, the amount of aldosterone produced was 55.7 pmol/nmol P-450/min in the absence of the lipidic extracts and the enhancing effect of the lipidic extracts was 4-fold.
D015069:18-Hydroxycorticosterone / Q000378:metabolism; D000302:Adrenal Cortex / Q000201:enzymology*; D000450:Aldosterone / Q000096:biosynthesis*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003345:Corticosterone / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D007700:Kinetics; D008055:Lipids / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology
D008055:Lipids; D000450:Aldosterone; D015069:18-Hydroxycorticosterone; D003577:Cytochrome P-450 Enzyme System; D003345:Corticosterone