title: A rapidly light-induced chloroplast protein with a high turnover coded for by pea nuclear DNA.
abstract: Among the translation products obtained in vitro with mRNAs isolated from etiolated grown pea, or after different times of illumination following the etiolation, a 24000-Mr protein has been observed in the very early phase of greening; its occurrence culminates at 2-4 h after the start of illumination. From these data it is concluded that the corresponding mRNA appears in and disappears from the poly(A)-containing RNA population within hours. The protein product has been characterized as the precursor for a 17000-Mr chloroplast protein; by means of post-translational transport in vitro, the processed product becomes bound to chloroplast membranes. A product of the same size can also be labeled in vivo with a maximum of incorporation of label at 6-8 h after illumination. This product decays with a half-life of about 5 h. These findings imply a regulatory function of the 17000-Mr protein during the process of greening, possibly by synchronization of nuclear and chloroplast genomes. Other possibilities are considered.
mesh_terms: D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism*; D004247:DNA / Q000235:genetics*; D007887:Fabaceae / Q000235:genetics / Q000378:metabolism*; D008027:Light; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D010946:Plants, Medicinal; D014176:Protein Biosynthesis / Q000528:radiation effects*; D012333:RNA, Messenger / Q000528:radiation effects
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D004247:DNA