Plasmids as epidemiological markers in nosocomial Legionnaires' disease.
Plasmid analysis was used in the investigation of an outbreak of nosocomial Legionnaires' disease in four patients. Serogroup 1 strains were isolated from two patients, the air-conditioning cooling tower, and two hot-water tanks. All serogroup 1 strains contained two plasmids with approximate molecular masses of 21 and 48 megadaltons (Mdal). The serogroup 1 strain found in the cooling-tower isolate also contained an additional 1.9 Mdal-plasmid. Restriction-endonuclease analysis of the 21-Mdal plasmid that was present in patient and hot water-tank isolates revealed identical EcoRI and HaeIII fragment patterns. Digestion of the similarly sized plasmid in the cooling-tower isolate resulted in a unique fragment pattern. The data provide direct bacteriologic evidence implicating the hot-water tanks rather than the cooling tower as the source of the infecting strain.
D003428:Cross Infection / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D007875:Legionella / Q000235:genetics; D007877:Legionnaires' Disease / Q000382:microbiology*; D010957:Plasmids; D013183:Sputum / Q000382:microbiology; D014871:Water Microbiology
D004269:DNA, Bacterial