The 5' ends of the transforming gene of Fujinami sarcoma virus and of the cellular proto-fps gene are not colinear.
The sequence of 500 nucleotides of a cellular proto-fps region that includes the 5' boundary of overlap with the fps sequence of Fujinami sarcoma virus (FSV) was determined. It was found that the potential reading frame of the proto-fps region continues over the 5' boundary of overlap with viral fps for 44 codons which do not include an AUG translation initiation codon. A potential upstream exon of proto-fps of over 91 codons and a potential splice acceptor at the site of fps/proto-fps overlap have been identified. It is concluded that the boundary of overlap between proto-fps and viral fps cannot represent the 5' end of the coding region of proto-fps. The proto-fps gene starts either in the upstream exon(s) not shared with FSV or at least 51 codons downstream of the boundary of overlap with FSV. In addition, the 5' coding region of proto-fps differs from the (delta gag-fps) hybrid transforming gene of FSV in the absence of the retroviral gag gene-derived sequence.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D005796:Genes; D005814:Genes, Viral; D009857:Oncogenes; D012190:Retroviridae / Q000235:genetics*