Iodide transport in a continuous line of cultured cells from rat thyroid.
The properties of TSH-dependent iodide (I-) uptake are defined for a cloned, continuously growing, functioning rat thyroid cell line (FRTL-5 cells). Since these cells grow without a lumen and are therefore restricted in their ability to iodinate thyroglobulin, the FRTL-5 cells offer the opportunity to directly study transport processes across the membrane into the cell as well as the process whereby I- moves from the cell. FRTL-5 cells concentrate I- approximately 30-fold and exhibit many of the properties of I- uptake seen in thyroid tissue slice and primary cell culture systems. In these cells, accumulation of I- is consistent with a Na+-dependent carrier model for I- uptake, and effects on the influx and efflux processes can be dissociated. Because FRTL-5 cells can be maintained in culture indefinitely and can provide large quantities of a homogeneous functional thyroid cell preparation for study, these cells offer the unique opportunity to further define the mechanism and kinetics of I- transport in a less complex system.
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002250:Carbon Radioisotopes; D002460:Cell Line; D006863:Hydrogen-Ion Concentration; D007454:Iodides / Q000378:metabolism*; D007457:Iodine Radioisotopes; D007700:Kinetics; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D013696:Temperature; D013961:Thyroid Gland / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology*
D000621:Aminoisobutyric Acids; D002250:Carbon Radioisotopes; D007454:Iodides; D007457:Iodine Radioisotopes; C100049:2-aminoisobutyric acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D013972:Thyrotropin; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D011188:Potassium