Mitogens and melittin stimulate an increase in intracellular free calcium concentration in human fibroblasts.
Intracellular free Ca+2 concentration was measured in cultured human fibroblasts (HSWP) utilizing the Ca+2-sensitive fluorescent probe quin2. The addition of peptide growth factors to serum-deprived HSWP cells induced an immediate rise in intracellular Ca+2 concentration. This mitogen-induced rise in Ca+2 concentration could be blocked by the addition of the intracellular Ca+2 antagonist TMB-8. Addition of the phospholipase activator, melittin, to cells in the absence of growth factors also caused a dramatic rise in intracellular Ca+2 concentration which was blocked by TMB-8.
D000634:Aminoquinolines; D001514:Bee Venoms / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D005456:Fluorescent Dyes; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D008297:Male; D008555:Melitten / Q000494:pharmacology*; D008934:Mitogens / Q000494:pharmacology*
D000634:Aminoquinolines; D001514:Bee Venoms; D002121:Calcium Channel Blockers; D005456:Fluorescent Dyes; D008934:Mitogens; D008555:Melitten; C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D005707:Gallic Acid; C035738:Quin2-acetoxymethyl ester; D002118:Calcium