[Synthesis of a high-volume immunosorbent with oriented immobilization of Fab'-fragments for antigen separation].
A highly capacious immunosorbent for antigen demonstration has been synthesized. Use has been made of oriented immobilization of antibody Fab'-fragments on porous cellulose balls at the expense of the reaction of disulfide exchange. Up to 100 mg of Fab' was fixed on the sorbent, with the capacity reaching 200 mg antigen (rabbit IgG) per g of dry weight sorbent. The molar ratio Fab'/IgG was equal to 0.8-0.9, i.e. approximated the theoretical limit: one molecule of antigen per one active center antibody.
D000936:Antigen-Antibody Complex; D000941:Antigens / Q000302:isolation & purification*; D007140:Immunoglobulin Fab Fragments; D007164:Immunosorbents; D008722:Methods
D000936:Antigen-Antibody Complex; D000941:Antigens; D007140:Immunoglobulin Fab Fragments; D007164:Immunosorbents
Sintez vysokoemkogo immunosorbenta s orientirovannoĭ immobilizatsieĭ Fab'-fragmentov dlia vydeleniia antigena.