Growth hormone dependency of the effect of human serum on thymidine uptake by lectin-activated human lymphocytes.
This study of the effect of serum dialysis on thymidine incorporation into lectin-activated blood lymphocytes cultured in vitro showed that (1) after serum dialysis 3H-thymidine incorporation is very low or absent; (2) the addition of L-glutamine to dialyzed sera leads to recovery of 18% of 3H-thymidine uptake, and (3) addition of L-glutamine reduces the difference in the stimulating effects of acromegalic and hypopituitary sera observed before dialysis in a medium free of L-glutamine. Since the plasma level of glutamine is increased in acromegalics and reduced in hypopituitary patients, it is suggested that this amino acid acts as a growth hormone dependent factor or cofactor in the regulation of cell growth.
D000172:Acromegaly / Q000097:blood; D000293:Adolescent; D000328:Adult; D004393:Dwarfism, Pituitary / Q000097:blood; D005973:Glutamine / Q000502:physiology*; D013006:Growth Hormone / Q000502:physiology*; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008875:Middle Aged; D006435:Renal Dialysis; D013936:Thymidine / Q000378:metabolism*
D037102:Lectins; D005973:Glutamine; D013006:Growth Hormone; D013936:Thymidine