FAD-induced in vitro activation of glutathione reductase in the lens of B2 deficient rats.
We studied the FAD-induced in vitro stimulation of lenticular glutathione reductase in riboflavin-deficient rats. The stimulatory effect of FAD on lenticular glutathione reductase in rats fed a B2-deficient diet for 4 weeks was remarkably higher than in paired control rats fed a B2-supplemented basal diet and control rats had ad libitum access to a B2-supplemented basal diet. The in vitro FAD stimulation effect on rat lenticular glutathione reductase represents a sensitive indicator of the B2 deficient status.
D000818:Animals; D004032:Diet; D005182:Flavin-Adenine Dinucleotide / Q000494:pharmacology*; D005980:Glutathione Reductase / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012256:Riboflavin / Q000008:administration & dosage; D012257:Riboflavin Deficiency / Q000175:diagnosis / Q000201:enzymology*
D005182:Flavin-Adenine Dinucleotide; D005980:Glutathione Reductase; D012256:Riboflavin