Malignant activation of a K-ras oncogene in lung carcinoma but not in normal tissue of the same patient.
A single genetic alteration, a guanine-to-cytosine transversion, is responsible for the acquisition of malignant properties by K-ras genes of two human tumor cell lines established from carcinomas of the bladder (A1698) and lung (A2182). As a consequence, arginine instead of the normal glycine is incorporated into the K-ras-coded p21 proteins at amino acid position 12. This mutation creates a restriction enzyme polymorphism that can be used to screen human cells for transforming K-ras genes. This approach was used to identify the mutational event responsible for the malignant activation of a K-ras oncogene in a squamous cell lung carcinoma of a 66-year-old man; this point mutation was not present in either the normal bronchial or parenchymal tissue or in the blood lymphocytes. Hence, malignant activation of a ras oncogene appears to be specifically associated with the development of a human neoplasm.
D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D004273:DNA, Neoplasm / Q000235:genetics; D005799:Genes, Dominant; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D009154:Mutation; D009857:Oncogenes; D009928:Organ Specificity; D011110:Polymorphism, Genetic
D004273:DNA, Neoplasm