Crossed radioimmunoelectrophoretic analysis of cultivated rye (Secale cereale) pollen allergens.
Cultivated rye pollen extracts were characterized by means of crossed immunoelectrophoresis (CIE) and crossed radioimmunoelectrophoresis (CRIE). 32 antigens were identified in CIE and among these 16 were radiostained in CRIE analysis of a rye pollen sensitive patient panel. Crossed line immunoelectrophoresis revealed that some of the rye pollen antigens were immunological partially identical with antigens of wheat flour and rye flour.
D000818:Animals; D000937:Antigen-Antibody Reactions; D001345:Autoradiography; D003429:Cross Reactions; D002523:Edible Grain / Q000009:adverse effects; D005512:Food Hypersensitivity / Q000209:etiology; D006801:Humans; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D010940:Plant Proteins / Q000032:analysis; D011058:Pollen / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D012434:Secale / Q000009:adverse effects
D010940:Plant Proteins