Aqueous solution structure of an intercalated actinomycin D-dATGCAT complex by two-dimensional and one-dimensional proton NMR.
Two-dimensional NOESY and COSY 1H NMR techniques have been employed to determine the conformation of the complex formed by actinomycin D and the hexanucleoside pentaphosphate dATGCAT. One-dimensional NOE experiments in H2O confirmed the intact nature of the oligonucleotide double helix within the complex. The drug chromophore was intercalated between the GC base pairs, with the pentapeptide lactones nestled in the minor groove. No significant conformational change of the pentapeptide lactones between bound and free drug was observed.
D003609:Dactinomycin; D007364:Intercalating Agents; D007783:Lactones; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D011487:Protein Conformation; D012996:Solutions
D007364:Intercalating Agents; D007783:Lactones; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D012996:Solutions; D003609:Dactinomycin; C040943:actinomycin D-dATGCAT complex