[Isolation and characteristics of immune complexes circulating in the serum of acute leukemia patients].
Immunoglobulin G-containing immune complexes are obtained from blood serum of patients with acute leukemia by the method which includes precipitation using polyethylene glycol-6000, Sephadex G-200 gel filtration and protein A-sepharose affinity chromatography (pH 8.5). Immunologically active antibodies and antigens of complexes are isolated by Sephadex G-200 gel chromatography under conditions of dissociation (the sodium-citrate buffer, pH 7.5, 9 M urea). The immune complexes with the antigens and antibodies isolated from them are partially characterized using disc-immunoelectrophoresis, isoelectrofocusing and immune-isoelectrofocusing.
D000208:Acute Disease; D000936:Antigen-Antibody Complex / Q000032:analysis*; D002648:Child; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D006801:Humans; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000032:analysis; D007525:Isoelectric Focusing; D007938:Leukemia / Q000097:blood* / Q000276:immunology
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G
Vydelenie i kharakteristika immunnykh kompleksov, tsirkuliruiushchikh v syvorotke krovi bol'nykh ostrym leĭkozom.