title: [Determination of free and bound ergosterol in microorganisms].
abstract: A method for the quantitative determination of free and esterified ergosterol in lipid fractions from biomasses grown on hydrocarbons as sole carbon source is described. Column chromatography was used for the fractionation of lipids. Esterified ergosterol was determined in the benzene fraction and free ergosterol in the methanol fraction by known methods.
mesh_terms: D002850:Chromatography, Gel; D004875:Ergosterol / Q000032:analysis*; D008055:Lipids / Q000032:analysis; D012997:Solvents; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D015003:Yeasts / Q000032:analysis*
chemical_list: D008055:Lipids; D012997:Solvents; D004875:Ergosterol
vernacular_title: Bestimmung von freiem und gebundenem Ergosterol in Mikroorganismen.