Storage of chemotherapy drugs for use in the human tumor stem cell assay.
Tumor chemosensitivity assays require the frequent in vitro use of antineoplastic drugs. Economy and convenience are greatly enhanced if these drugs are stored in aliquots for use as needed. This study investigated the stability of eight common antineoplastic agents at -60 degrees C. Activity was measured as inhibition of colony formation in a commonly used soft agar culture system using an experimentally induced murine sarcoma. Our results indicate no loss of activity with these drugs under the specified storage conditions.
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D003114:Colony-Forming Units Assay; D004356:Drug Storage; D006801:Humans; D066298:In Vitro Techniques; D051381:Rats; D012513:Sarcoma, Experimental / Q000188:drug therapy; D014410:Tumor Stem Cell Assay
D000970:Antineoplastic Agents