Nucleotide sequence at the termini of the DNA of Streptococcus pneumoniae phage Cp-1.
The 5' ends of Cp-1 DNA, which have a covalently linked terminal protein, can be partially unblocked by treatment with 1 M NaOH (E. Garcia, A. Gomez, C. Ronda, C. Escarmis, and R. Lopez (1983) Virology 128, 92-104) and labeled with polynucleotide kinase and [gamma-32P]ATP. The sequence of the first 444 and 520 nucleotides at the termini of Cp-1 DNA has been determined. A 236-nucleotide-long inverted terminal repeat was found and, in addition, the 116 nucleotides following the repeat show 93% homology. The first 352 nucleotides at both ends have an adenine plus thymine content of 75%. More than 50% of the nucleotides of the sequenced regions are involved in repeats of a minimum of 8 nucleotides. Three promoter-like sequences were also found at each end of Cp-1 DNA.
D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D003852:Deoxyribonucleoproteins / Q000235:genetics; D009838:Oligodeoxyribonucleotides / Q000302:isolation & purification; D012091:Repetitive Sequences, Nucleic Acid; D013296:Streptococcus pneumoniae
D004279:DNA, Viral; D003852:Deoxyribonucleoproteins; D009838:Oligodeoxyribonucleotides