Chemical characterization of cyanogen bromide fragments from the beta-chain of human complement factor C3.
The isolated beta-chain of human complement factor C3 (C3 beta) was fragmented by cyanogen bromide. Nine fragments were defined by gel filtration and high-pressure liquid chromatography, and characterized with respect to their Mr, amino acid composition and N-terminal amino acid sequence. Approx. 30% of the primary structure of C3 beta was determined. Alignment of the 3 N-terminal fragments allowed determination of 61 of the amino terminal residues of C3 beta. This region demonstrated 40% homology with the sequence in the N-terminal segment of the alpha-chain of the cobra venom factor.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003176:Complement C3 / Q000032:analysis*; D003488:Cyanogen Bromide; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis*
D000596:Amino Acids; D003176:Complement C3; D010446:Peptide Fragments; D003488:Cyanogen Bromide