Accumulation of galactonate in liver of suckling rats perfused with galactose.
Livers of 14-day-old suckling rats were perfused for 30 min in the nonrecirculating (once-through) mode with media containing 0-60 mM galactose. Galactonate was detected in livers perfused with as little as 1 mM galactose and increased with higher perfusate galactose to 178 nmoles per gram of liver at 60 mM hexose. Tissue galactonate levels were as high as 60% of galactose-1-phosphate which also accumulated under the same conditions. Galactonate was also found to be present in the effluent perfusate. The perfused suckling rat liver appears to be a physiological model for assessing the role of galactose oxidation to galactonate as an alternate metabolic pathway of galactose metabolism.
D000818:Animals; D000833:Animals, Suckling / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D005695:Galactosephosphates / Q000302:isolation & purification; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013400:Sugar Acids / Q000302:isolation & purification / Q000378:metabolism*
D005695:Galactosephosphates; D013400:Sugar Acids; C012991:galactonic acid; C029973:galactose-1-phosphate; D005690:Galactose