Effects of diabetes on glucose oxidation in rat aorta after hypophysectomy and adrenalectomy.
The influence of diabetes, hypophysectomy and adrenalectomy on glucose oxidation in rat aorta was studied. Diabetes was induced in normal, adrenalectomized and hypophysectomized-cortisone substituted rats by streptozotocin (65 mg/kg body weight). The oxidation of glucose to CO2 was determined during incubation of rat aorta in vitro for 2-3 hours. The aortic glucose oxidation was reduced after hypophysectomy but was unaffected by adrenalectomy. After streptozotocin treatment the rise in blood glucose concentration was similar in normal, adrenalectomized and hypophysectomized-cortisone substituted rats. In shamoperated diabetic rats the aortic glucose oxidation was reduced after a diabetes duration of 4 days. In adrenalectomized diabetic rats the aortic glucose oxidation was not significantly affected after 4 days but was reduced after a diabetes duration of 14 days. When adrenalectomized diabetic rats were treated with hydrocortisone the aortic glucose oxidation was reduced after diabetes for 4 days. After incubation of normal rat aorta in vitro for 6 hours with cortisol (1 microgram/ml) in the incubation medium a decrease in the aortic glucose oxidation was found. Incubation of aorta with only growth hormone had no effect. These results suggest that cortisol is of importance for the lowered glucose oxidation in diabetic rat aorta.
D020155:3-Hydroxybutyric Acid; D000311:Adrenal Glands / Q000502:physiology*; D000315:Adrenalectomy; D000818:Animals; D001011:Aorta / Q000201:enzymology; D001786:Blood Glucose / Q000378:metabolism; D001808:Blood Vessels / Q000378:metabolism*; D003348:Cortisone / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005947:Glucose / Q000378:metabolism*; D013006:Growth Hormone / Q000502:physiology; D006854:Hydrocortisone / Q000502:physiology; D006885:Hydroxybutyrates / Q000097:blood; D007016:Hypophysectomy; D008297:Male; D010084:Oxidation-Reduction; D010902:Pituitary Gland / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D006885:Hydroxybutyrates; D013006:Growth Hormone; D005947:Glucose; D020155:3-Hydroxybutyric Acid; D003348:Cortisone; D006854:Hydrocortisone