Functional association of a monoacylglycerophosphocholine acyltransferase and the oleoylglycerophosphocholine desaturase in microsomes from developing leaves.
The biosynthesis of linoleic acid has been investigated, using oleoyl-CoA as a substrate, in microsomal preparations from young leaves of Pisum sativum. Oleoyl moieties from oleoyl-CoA were preferentially acylated to lysophosphatidylcholine by an acyltransferase to produce an oleoylglycerophosphocholine. Kinetic data are presented which argue for a direct desaturation of the oleoyl moieties of this oleoyl glycerophosphocholine to linoleoyl moieties. There was no evidence of a subsequent acyltransfer of linoleoyl moieties either to form thioesters or oxygen esters in other complex lipids. The kinetics were also consistent with a functional coupling of the lysophosphatidylcholine acyltransferase with the oleate desaturase. There was little exchange of the oleoyl glycerophosphocholine from the bulk membrane lipid with that newly synthesised by the lysophosphatidylcholine acyltransferase. Rather, the newly synthesised oleoylglycerophosphocholine seemed to be directly channelled to the vicinity of the desaturase. The results are discussed in the context of 'metabolite channelling'. The consequences for desaturase activity and its regulation are also examined.
D008243:1-Acylglycerophosphocholine O-Acyltransferase / Q000378:metabolism*; D000214:Acyl Coenzyme A / Q000378:metabolism; D000217:Acyltransferases / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D007887:Fabaceae / Q000201:enzymology* / Q000254:growth & development; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D007700:Kinetics; D008563:Membrane Lipids / Q000378:metabolism; D008861:Microsomes / Q000201:enzymology*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010946:Plants, Medicinal; D013379:Substrate Specificity
D000214:Acyl Coenzyme A; D008563:Membrane Lipids; C017585:oleoyl-coenzyme A; D044943:Fatty Acid Desaturases; C021904:Phosphatidylcholine desaturase; D044925:Oxidoreductases Acting on CH-CH Group Donors; D000217:Acyltransferases; D008243:1-Acylglycerophosphocholine O-Acyltransferase