Isolation and characterization of the rabbit prt gene product.
The product of the rabbit prt gene (PRT), a gene linked to the immunoglobulin kappa-light chain gene ab, was purified from rabbit serum by precipitation with ammonium sulfate and by chromatography on DEAE-Sephadex and Sephacryl S300. Analysis of PRT indicated that it was associated rabbit hemopexin; the molecular weight of PRT (i.e., 68,000), as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was similar to the reported molecular weight of rabbit hemopexin; the PRT phenotypes correlated with the phenotypes of a hematin binding protein; PRT itself bound hematin; and the amino acid composition of PRT was similar to the amino acid composition of rabbit hemopexin. The prt gene, however, need not be the structural gene for hemopexin; it may encode a glycosyl transferase responsible in part for the carbohydrate associated with the protein.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D005815:Genetic Code; D006466:Hemopexin / Q000235:genetics / Q000302:isolation & purification*; D007120:Immunochemistry; D010641:Phenotype; D014176:Protein Biosynthesis; D011817:Rabbits
D000596:Amino Acids; D006466:Hemopexin