The occurrence of ecdysteroids in the cestode, Moniezia expansa.
The occurrence of free ecdysteroids in the sheep cestode, Moniezia expansa, was demonstrated. Significant amounts of conjugated ecdysteroids were not detected. Characterization of the free hormones by high-performance liquid chromatography monitoring fractions by radioimmunoassay, and by gas chromatography/mass spectrometry (selected ion monitoring) indicated the presence of ecdysone, 20-hydroxyecdysone and 20,26-dihydroxyecdysone. Analysis of the ecdysteroids by radioimmunoassay in segments along part of the strobila indicated that the anterior parts contained the greatest amount of hormone. GC/MS (SIM) analysis of the hormones in a strobilar segment containing the most mature proglottids suggested the presence of several ecdysteroid metabolites.
D000818:Animals; D002589:Cestoda / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D026461:Ecdysteroids; D008401:Gas Chromatography-Mass Spectrometry; D007447:Invertebrate Hormones / Q000032:analysis* / Q000302:isolation & purification; D008989:Monieziasis; D011863:Radioimmunoassay
D026461:Ecdysteroids; D007447:Invertebrate Hormones