[Metabolic disorder of various protein fractions in the thymocytes of irradiated rats (an electrophoretic analysis and proteins interacting with DNA].
Using PAAG-electrophoresis and subsequent fluorography no essential disorders were detected in the biosynthesis of some protein fractions of the cytoplasm and nuclear sap of thymocytes during the first 2 h following irradiation of rats with a dose of 8 Gy. There was a decrease, progressing in time, in the rate of the synthesis of certain polypeptides. Inhibition of the biosynthesis of the proteins reacting with DNA was demonstrated by the chromatography, on DNA-containing columns, of labeled proteins of different fractions. The most pronounced changes were observed in the group of firmly bound proteins.
D000818:Animals; D004247:DNA / Q000032:analysis / Q000378:metabolism / Q000528:radiation effects*; D004347:Drug Interactions / Q000528:radiation effects; D004591:Electrophoresis, Polyacrylamide Gel; D005720:Gamma Rays; D008297:Male; D011506:Proteins / Q000032:analysis / Q000378:metabolism / Q000528:radiation effects*; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence; D013950:Thymus Gland / Q000378:metabolism / Q000528:radiation effects*
D011506:Proteins; D004247:DNA
Narushenie metabolizma razlichnykh belkovykh fraktsiĭ v timotsitakh obluchennykh krys (élektroforeticheskiĭ analiz i belki, vzaimodeĭstvu-iushchie s DNK).