Monoclonal antibody ECCD-1 inhibits intercellular communication in teratocarcinoma PCC3 cells.
The monoclonal antibody ECCD-1 recognizing a certain class of cell surface proteins inhibits the Ca2+-dependent cell-to-cell adhesion in teratocarcinoma stem cells. In this paper, we studied the effect of ECCD-1 on cell-to-cell communication in PCC3 cells by measuring the transfer of lucifer yellow between cells. To this aim, PCC3 cells were cultured in the presence of ECCD-1 for various periods, and then the fluorescent dye was injected into a cell located in the center of cell colonies, followed by counting number of cells to which the dye was transferred. The results showed that ECCD-1 inhibits the dye transfer between cells, suggesting that the Ca2+-dependent cell-to-cell adhesion system (CDS) is essential for the functions of gap junction.
D000818:Animals; D000911:Antibodies, Monoclonal; D002448:Cell Adhesion; D002450:Cell Communication; D002460:Cell Line; D007546:Isoquinolines / Q000378:metabolism; D008565:Membrane Proteins / Q000276:immunology / Q000502:physiology*; D009363:Neoplasm Proteins / Q000276:immunology / Q000502:physiology*; D013724:Teratoma / Q000503:physiopathology*
D000911:Antibodies, Monoclonal; D007546:Isoquinolines; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C017475:lucifer yellow