Influence of a fibroblastoid cell line derived from murine bone marrow (H-1 cells) on stem cell proliferation.
A murine fibroblastoid cell line (H-1) with properties similar to those of adventitial reticular cells can support granulopoiesis and the development of mononuclear phagocytes in vitro. In the current study the effect of these cells on stem cell maintenance in vitro was assessed. The H-1 cells were unable to support CFUs replication in liquid culture, while treatment of some stem cells with H-1 conditioned medium appeared to inhibit their proliferation.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D003114:Colony-Forming Units Assay; D003470:Culture Media; D005347:Fibroblasts; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013997:Time Factors
D003470:Culture Media