Determination of biogenic amines in meat by combined ion-exchange and capillary gas chromatography.
A procedure is described for the determination of putrescine, cadaverine and histamine in meat. Crude perchloric acid extracts were pre-separated on a weakly acidic cation exchanger and the amines quantified by capillary gas chromatography. The diamines were analysed as trifluoroacetyl derivatives and histamine was converted into N alpha-trifluoroacetyl-N tau-ethoxycarbonylhistamine. The accuracy of the determination of diamines was examined by a precipitation pre-separation method and by mass fragmentometric quantification. The proposed procedure allows the sensitive, sufficiently precise and highly specific determination of putrescine and cadaverine in meat.
D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D002103:Cadaverine / Q000032:analysis; D011232:Chemical Precipitation; D002849:Chromatography, Gas / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006632:Histamine / Q000032:analysis; D008460:Meat / Q000032:analysis*; D011700:Putrescine / Q000032:analysis; D013552:Swine; D013775:Tetraphenylborate; D014269:Trifluoroacetic Acid / Q000138:chemical synthesis
D001679:Biogenic Amines; D013775:Tetraphenylborate; D006632:Histamine; D014269:Trifluoroacetic Acid; D002103:Cadaverine; D011700:Putrescine