Radioimmunoassay for psychotropic drugs. III: Synthesis and properties of haptens for trifluoperazine and fluphenazine.
For the development of radioimmunoassay procedures for trifluoperazine and fluphenazine, three haptens, N-(2-carboxyethyl)desmethyltrifluoperazine, N-(4-carboxybutyl)desmethyltrifluoperazine, and 10-[3-(4-carboxyethylpiperazinyl)-3-oxopropyl]-2-trifluoromethyl-+ ++10H- phenothiazine, were synthesized and characterized. Each hapten was coupled to bovine serum albumin, and the number of hapten residues per mole of bovine serum albumin was calculated by UV spectrophotometric methods. Antibodies to each hapten-protein conjugate were developed in rabbits, and titers of the antisera were checked by evaluating their binding characteristics to the tritiated drug.
D000818:Animals; D002417:Cattle; D005476:Fluphenazine / Q000032:analysis*; D006241:Haptens / Q000138:chemical synthesis; D011863:Radioimmunoassay / Q000379:methods; D012710:Serum Albumin, Bovine / Q000032:analysis; D014268:Trifluoperazine / Q000032:analysis*
D006241:Haptens; D014268:Trifluoperazine; D012710:Serum Albumin, Bovine; D005476:Fluphenazine