[Purification and comparative study of the properties of glucocorticoid- and estrogen-receptor complexes in the rat liver].
Parallel purification of glucocorticoid- and estrogen-receptor complexes from rat liver cytosol has been accomplished. Some properties of purified steroid-receptor complexes (SRC) were determined. The procedure developed earlier when the two-step treatment of cytosol with DNA-cellulose alternated with SRC ammonium sulphate precipitation, was shown to be universally applicable for purification of various SRC. Certain modifications have been devised allowing some increase in the degree of receptor purification. The amount of estrogen-receptor complexes (ERC) isolated from male liver cytosol was 20-40 times less than that of glucocorticoid-receptor complexes (GRC) isolated simultaneously from the equal volume of the same cytosol. Both SRC types bind intensively to homologous DNA but not to poly(A). The elution of GRC from DNA cellulose was mainly achieved at 0.4 M NaCl. With this, GRC and ERC showed small, but reliable differences in the salt resistance of their associates with DNA: the ERC-DNA link was stable toward NaCl up to 0.1 M, whereas an appreciable amount of GRC was eluted from DNA-cellulose at 0.1 M NaCl. The stability of purified ERC exceeded that of purified GRC, which apparently reflects the differences in the hormone-receptor binding constants. The receptor stability under various environmental conditions is discussed and some recommendations on the improvement of the SRC stability and its control are given.
D000818:Animals; D003600:Cytosol / Q000032:analysis*; D004247:DNA / Q000378:metabolism; D005260:Female; D008099:Liver / Q000378:metabolism*; D008297:Male; D000090204:Mesothelin; D051381:Rats; D011960:Receptors, Estrogen / Q000302:isolation & purification* / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000302:isolation & purification* / Q000378:metabolism; D011987:Receptors, Steroid / Q000302:isolation & purification*
C000715529:Msln protein, rat; D011960:Receptors, Estrogen; D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D004247:DNA; D000090204:Mesothelin
Ochistka i sravnitel'noe izuchenie svoĭstv gliukokortikoid- i éstrogen-retseptornykh kompleksov pecheni krys.