The enzymatic determination of urea using the Greiner G 300.
A diagnostic kit for the enzymatic determination of urea was evaluated. The with-in-run had a coefficient of variation of 1.9-2.4%. The day-to-day run precision was found to have a coefficient of variation of 2.1 - 3.5%. The mean analytical recovery was 97.5%. A correlation study was done with the Technicon Mark II method. Good linearity was found up to 30 mmol/l; after the addition of NADH linearity was extended up to 60 mmol/l. The working reagent was stable for two days.
D006801:Humans; D009243:NAD / Q000032:analysis; D011933:Reagent Kits, Diagnostic; D013056:Spectrophotometry, Ultraviolet; D014508:Urea / Q000032:analysis*; D014510:Urease
D011933:Reagent Kits, Diagnostic; D009243:NAD; D014508:Urea; D014510:Urease