DNA damage induced in human diploid cells by decay of incorporated radionuclides.
Alkaline and neutral elution techniques were used to characterize the production of single- and double-strand DNA breaks in human diploid fibroblasts by incorporated radionuclides. 125I was incorporated in DNA as [125I]iododeoxyuridine, 3H as [3H]-thymidine, and 14C as [14C]thymidine. Under frozen conditions, 125I was 3 times as efficient as 3H per decay in inducing single-strand breaks and 6 times as efficient as 14C. For double-strand break production, however, 125I was 6 times as efficient per decay as 3H. It was calculated that, on the average, each 125I decay produces about one double-strand break in the frozen state. Under nonfrozen conditions, 125I and external X-rays were roughly 5-fold and 3H about 3-fold more efficient in double-strand break induction than under frozen conditions.
D002250:Carbon Radioisotopes; D002460:Cell Line; D004261:DNA Replication / Q000528:radiation effects*; D004171:Diploidy; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D007065:Idoxuridine / Q000378:metabolism*; D007457:Iodine Radioisotopes; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium
D002250:Carbon Radioisotopes; D007457:Iodine Radioisotopes; D014316:Tritium; D007065:Idoxuridine; D013936:Thymidine