Alteration of ceramide monohexoside in human diploid fetal lung fibroblasts during cell aging.
Serially cultured human diploid fibroblasts have a finite lifetime in vitro, and this phenomenon was postulated as cellular aging. Neutral glycosphingolipids (GSLs) from human fetal lung-derived diploid fibroblast cell lines, WI-38 and TIG-1 cells, were studied during cellular aging. Both cell lines had at least four types of neutral GSLs. It was found that neutral GSLs changed with aging, the most conspicuous alteration being a 2-4-fold increase in the content of ceramide monohexoside. This change was invariably observed in either WI-38 or TIG-1 cells.
D002460:Cell Line; D002470:Cell Survival; D002554:Cerebrosides / Q000032:analysis*; D002855:Chromatography, Thin Layer; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D006028:Glycosphingolipids / Q000032:analysis; D006801:Humans; D008168:Lung / Q000166:cytology*; D011247:Pregnancy
D002554:Cerebrosides; D006028:Glycosphingolipids; C013870:ceramide monohexoside