High-performance liquid chromatographic method for the determination of N-methylated metabolites of nicotine.
An analytical method has been developed, using cation-exchange high-performance liquid chromatography, for the analysis of N-methylated metabolites of nicotine. This method has been used to detect and quantitate seven potential in vivo urinary metabolites of [2'-14C]-nicotine, including four methylated nicotine derivatives, in the guinea pig.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D008745:Methylation; D009538:Nicotine / Q000031:analogs & derivatives* / Q000652:urine; D010084:Oxidation-Reduction
D009538:Nicotine