Effect of ethanol on the uptake of pyridoxine X HCl in the rat jejunum.
The effects of acute ethanol exposure and chronic ethanol ingestion on jejunal mucosal uptake of 2 microM pyridoxine X HCl (PN) was studied in rats. Experimental models included in vitro everted sacs and in vivo perfused segments. The presence of 4% w/v but not 1% w/v ethanol (mucosal side) acutely enhanced PN uptake by 30 to 76%. Ethanol removal rapidly returned uptake rates to base-line. Histological evidence of mucosal injury in perfused segments paralleled observed changes in PN uptake. After chronic ethanol ingestion (1 yr), PN uptake from media without added ethanol was not statistically different from control values. Conclusions are: 1) acutely, enhancement of PN mucosal uptake by ethanol in rat jejunum is associated with the presence of a high ethanol concentration, is rapidly reversible, and correlates with histological evidence of mucosal injury; and 2) chronic ingestion of ethanol appears to have no further effect on PN uptake in the rat.
D000818:Animals; D000431:Ethanol / Q000494:pharmacology*; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000187:drug effects; D007583:Jejunum / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011736:Pyridoxine / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000431:Ethanol; D011736:Pyridoxine