title: First demonstration of highly specific and sensitive antibodies against dopamine.
abstract: Dopamine was coupled to bovine serum albumin (BSA) with glutaraldehyde, precautions were taken in order to preserve the catechol ring. After injection of this immunogen into rabbits, anti-dopamine antibodies were obtained and tested using radioimmunochemical binding studies and adsorption to catecholamine covered sepharose beads. A good correlation was found between the results of the different test systems, allowing us to visualize dopamine specifically in glutaraldehyde-fixed rat brains.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001921:Brain / Q000276:immunology*; D004298:Dopamine / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay
chemical_list: D000906:Antibodies; D004298:Dopamine