Direct isolation of neuronal microtubule skeletons.
The microtubule skeletons of cultured neuronal cells have been isolated in their original form utilizing taxol and rigorous extraction conditions. Continuous microtubules are visible throughout the cell body and into the processes. These preparations include the complex set of microtubule-associated proteins of 69,000 and 80,000 daltons previously identified in these cells.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D003593:Cytoplasm / Q000378:metabolism; D051379:Mice; D008870:Microtubules / Q000648:ultrastructure*; D009447:Neuroblastoma / Q000648:ultrastructure; D009474:Neurons / Q000648:ultrastructure*; D010455:Peptides / Q000032:analysis
D010455:Peptides