Headspace sampling and GC analysis of volatile urinary metabolites.
A method requiring only 1 to 3 ml of sample is developed for the analysis of volatile metabolites in urine. Trace volatiles are stripped with helium and concentrated on Porapak Q and activated charcoal traps in series. Volatiles are thermally desorbed into a fused silica capillary column for analysis by gas chromatography. Variables affecting the process are studied. Profiles of untreated and acid hydrolyzed urine are presented.
D000327:Adsorption; D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D013696:Temperature; D014556:Urine / Q000032:analysis*; D014835:Volatilization