Influence of histone hyperacetylation on nucleosomal particles as visualized by electron microscopy.
Recently, Bode et al. [J. Bode, M. Gómez-Lira, and H. Schröter (1983) Eur. J. Biochem. 130, 437-445] have observed that monomeric nucleosomal particles from butyrate-treated Namalva lymphoma cells display a distinct heterogeneity in their mobilities on a nondenaturing 4% polyacrylamide gel. They have proposed that histone hyperacetylation induces a conformational change in monomers that can be modulated by the presence of HMG 14/17. The electron microscopic analyses presented here support these proposals.
D000107:Acetylation; D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002460:Cell Line; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification; D004247:DNA / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000302:isolation & purification / Q000378:metabolism*; D008223:Lymphoma / Q000648:ultrastructure; D008854:Microscopy, Electron; D008968:Molecular Conformation; D009707:Nucleosomes / Q000032:analysis / Q000648:ultrastructure*
D002087:Butyrates; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D009707:Nucleosomes; D020148:Butyric Acid; D004247:DNA