Nucleophile specificity in chymotrypsin peptide synthesis.
The partitioning of the acylenzyme acetyl-(Gly)n-Phe(NO2)-chymotrypsin (n = 0,1,2) to peptide and peptide acid is observed spectrophotometrically. Values of partitioning ratios for various nucleophiles are calculated from the spectral data. They are a measure for the "true" nucleophile reactivity and are useful in the prediction of the best experimental conditions in enzymic peptide synthesis. A large difference in the nucleophile reactivity is observed which is attributed to the S'2-P'2 interaction.
D000818:Animals; D002417:Cattle; D002918:Chymotrypsin / Q000378:metabolism*; D007202:Indicators and Reagents; D007700:Kinetics; D010179:Pancreas / Q000201:enzymology; D010455:Peptides / Q000138:chemical synthesis*; D013379:Substrate Specificity
D007202:Indicators and Reagents; D010455:Peptides; D002918:Chymotrypsin