A method for preparing two-dimensional surface-spreads of synaptonemal complexes from plant meiocytes for light and electron microscopy.
A method has been developed for preparing two-dimensional surface spreads of synaptonemal complexes (SCs) from plant meiocytes for examination by light and/or electron microscopy. Clear, well-spread preparations of SCs and unpaired axial cores have been obtained from a range of meiotic prophase I stages (leptotene to pachytene) from Allium and Secale meiocytes.
D001901:Botany / Q000379:methods*; D002467:Cell Nucleus / Q000648:ultrastructure; D002875:Chromosomes / Q000648:ultrastructure; D008540:Meiosis; D008853:Microscopy; D008854:Microscopy, Electron; D010944:Plants / Q000648:ultrastructure*