Colorimetry of hemoglobin in plasma with 2,2'-azino-di(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS).
Hemoglobin in plasma can be determined by the color-developing action of 2,2'-azino-di(3-ethylbenzthiazoline-6-sulfonic acid), which is oxidized to a colored form by a peroxidase-like effect of hemoglobin in the presence of hydrogen peroxide. Sensitivity, precision, and accuracy are discussed. The calibration curve is linear for hemoglobin concentrations up to 1 g/L; the minimum detectable concentration is 20 mg/L. The within-run precision (CV) was 2.39%, analytical recovery 101.8%. Interference from plasma proteins and lipids was eliminated by centrifuging the reaction mixture before measuring its absorbance at 410 nm.
D000925:Anticoagulants / Q000097:blood; D052160:Benzothiazoles; D001663:Bilirubin / Q000097:blood; D003124:Colorimetry / Q000379:methods*; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D013451:Sulfonic Acids
D000925:Anticoagulants; D052160:Benzothiazoles; D006454:Hemoglobins; D007202:Indicators and Reagents; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D001663:Bilirubin