Brain and pituitary fibroblast growth factor activities behave identically on three independent high performance liquid chromatography systems.
Fibroblast Growth Factors obtained from bovine brain and pituitary glands were compared. They were shown to behave identically on three high performance liquid chromatographic systems, which separate proteins by the independent criteria of size, charge and hydrophobicity. They also migrated similarly on electrophoresis in polyacrylamide gels in the presence of sodium dodecyl sulphate with an apparent molecular weight of 12,000. It was concluded that the two factors were very similar, or identical. They were active at promoting DNA synthesis in rat mammary fibroblasts (Rama 27) at a concentration of 0.1 ng/ml.
D000818:Animals; D001681:Biological Assay; D001923:Brain Chemistry; D002417:Cattle; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005346:Fibroblast Growth Factors / Q000302:isolation & purification*; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism; D008970:Molecular Weight; D010902:Pituitary Gland / Q000032:analysis*; D051381:Rats
D005346:Fibroblast Growth Factors; D004247:DNA