Increase in the complement-fixing ability of murine IgG anti-lymphocyte antibodies by addition of monoclonal IgM rheumatoid factors.
Mouse monoclonal IgM anti-IgG autoantibodies (rheumatoid factors) markedly enhanced the complement fixing ability of 2 murine IgG1 monoclonal antibodies against human lymphocytes (anti-Leu 4 and T305). The IgM rheumatoid factors were effective when added simultaneously with the monoclonal anti-lymphocyte antibodies, and were not themselves toxic towards human lymphocytes. Monoclonal IgM rheumatoid factors are simple reproducible reagents for amplifying complement fixation by murine IgG hybridoma antibodies.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D000961:Antilymphocyte Serum / Q000502:physiology*; D003168:Complement Fixation Tests; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology / Q000502:physiology; D007075:Immunoglobulin M / Q000502:physiology*; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D012217:Rheumatoid Factor / Q000502:physiology*
D000911:Antibodies, Monoclonal; D000961:Antilymphocyte Serum; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012217:Rheumatoid Factor