Visualization of reactive oxygen species formation by phagocytizing macrophages.
Activated peritoneal macrophages exhibiting phagocytosing capacity produced an electron-dense precipitate of formazan in contact sites of macrophage plasmalemma and phagocytosed yeast cells. No production of formazan occurred, when non-opsonized latex particles were ingested by macrophages. Formazan precipitation could be prevented by anaerobiosis but not by addition of cyanide.
D000693:Anaerobiosis; D000818:Animals; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D010100:Oxygen / Q000378:metabolism*; D010587:Phagocytosis; D013778:Tetrazolium Salts
D013778:Tetrazolium Salts; C028130:tetranitrotetrazolium blue; D010100:Oxygen