[Long-term culture of the rat medial aorta with preservation of the characteristic properties of differentiated smooth muscle vascular cells].
The authors provide the results of a long-term cultivation (106 passages during 2 years) of smooth muscle cells of rat aorta media, where the cells preserve the signs characteristic for their differentiation: ability to contract, specific ultrastructure (the presence of the caveole series, microfilaments of 4-6 nm, intermediate filaments of 10 nm, dark bodies in the cytoplasm), and ability to synthesize the alpha-form of actin specific for smooth muscle vascular cells.
D000818:Animals; D001011:Aorta / Q000166:cytology*; D002454:Cell Differentiation; D046508:Culture Techniques / Q000379:methods; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
Dlitel'noe kul'tivirovanie medii aorty krys s sokhraneniem kharakternykh svoĭstv differentsirovannykh gladkomyshechnykh sosudistykh kletok.