An examination of the efficiency of glucose and glutamine as energy sources for cultured chick pigment epithelial cells.
Embryonic chick pigment epithelial cells in culture require glucose as their major energy source for long-term growth, pigment formation, and colony organization. Cell number increases with glucose concentration at least up to 5.0 mM. Cells can be grown with glutamine as the major energy source but produce comparable cell numbers for only the first 3 days in culture, after which they cease growing. However, they are able to metabolize glutamine at a two to sixfold higher rate than cells grown in the presence of glucose as measured by CO2 release and by incorporation into protein. In cells grown in the presence of both glucose and glutamine, basal ATP levels were 31.1 nmoles/mg protein; P-creatine averaged 15.2 nmoles/mg protein and showed marked variability between experimental groups. During starvation, P-creatine levels fell while ATP levels remained relatively constant. Glucose was required for the recovery of P-creatine to prestarvation levels when measured 5 min after refeeding. Because of these marked changes in P-creatine concentration as a function of nutritional status, the ATP/P-creatine ratio becomes a useful measure of the energy state of the cell.
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D002642:Chick Embryo / Q000378:metabolism*; D003401:Creatine / Q000032:analysis; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D005973:Glutamine / Q000378:metabolism* / Q000494:pharmacology; D010857:Pigment Epithelium of Eye / Q000032:analysis / Q000166:cytology / Q000378:metabolism*
D005973:Glutamine; D000255:Adenosine Triphosphate; D005947:Glucose; D003401:Creatine