An improved in vitro immunization procedure for the production of monoclonal antibodies against neural and other antigens.
A simplified in vitro immunization procedure has been developed for the production of monoclonal antibodies against a variety of neural antigens. The procedure, which can be applied to both soluble and membrane-bound antigens, involves the addition of an adjuvant peptide, N-acetylmuramyl-L-alanyl-D-isoglutamine, and has been found to improve all fusion parameters.
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000008:administration & dosage*; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002417:Cattle; D002478:Cells, Cultured; D005968:Glutamate Decarboxylase / Q000276:immunology; D006624:Hippocampus / Q000276:immunology; D006825:Hybridomas / Q000276:immunology*; D009420:Nervous System / Q000276:immunology*; D051381:Rats
D000911:Antibodies, Monoclonal; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D005968:Glutamate Decarboxylase