mRNA sequences define an unusually restricted IgG response to oxazolone.
The idiotypic analysis of hybridomas derived 7 and 14 days after primary immunization with oxazolone suggested that V-gene expression at these two stages was very different mRNA H and L sequences disclosed that day-7 antibody structures were highly conserved, which can be attributed to the existence of one VH and one VL gene. Conversely, two Ox-id- sequences differed considerably from Ox-id+ antibodies and were apparently unrelated to the previously defined genes.
D000818:Animals; D000916:Antibody Diversity; D001483:Base Sequence; D005786:Gene Expression Regulation; D007074:Immunoglobulin G / Q000235:genetics*; D007130:Immunoglobulin Idiotypes / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D010080:Oxazoles / Q000276:immunology*; D010081:Oxazolone / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis*
D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D010080:Oxazoles; D012333:RNA, Messenger; D010081:Oxazolone