Biochemical characterization of a human parvovirus.
The buoyant density, nucleic acid, and proteins of the human serum parvovirus-like agent were investigated. Evidence is presented which suggests that the virus has genomic single-stranded DNA, and that complementary strands may be encapsidated in separate virions. Three proteins of 48 000, 68 000 and 80 000 mol. wt. were found to co-purify with viral antigen at a density of 1.43 g/ml on CsCl gradients. On the basis of these properties it is suggested that this virus is a parvovirus.
D002499:Centrifugation, Density Gradient; D004277:DNA, Single-Stranded / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008970:Molecular Weight; D010321:Parvoviridae / Q000032:analysis / Q000378:metabolism*; D012367:RNA, Viral / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins