Autoradiographic localization of [14C]8-S-adenosyl-L-homocysteine in rat brain.
Following intracarotid injection of [14C]8-S-adenosyl-L-homocysteine ( [14C]SAH) in the rat, radioactivity was localized by autoradiographic detection in the circumventricular organs, such as the pineal gland, choroid plexuses and area postrema, which lie outside the blood-brain barrier. HPLC analysis indicated a decrease of [14C]SAH radioactivity in these structures from 1 to 45 min, after injection. These results are discussed and related to the known pharmacological properties of SAH.
D000818:Animals; D001345:Autoradiography; D001812:Blood-Brain Barrier; D001921:Brain / Q000378:metabolism*; D002552:Cerebral Ventricles / Q000378:metabolism; D002831:Choroid Plexus / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006710:Homocysteine / Q000031:analogs & derivatives*; D008297:Male; D010870:Pineal Gland / Q000378:metabolism; D051381:Rats; D012435:S-Adenosylhomocysteine / Q000378:metabolism*
D006710:Homocysteine; D012435:S-Adenosylhomocysteine