Cleavage of formate from omega,4-dihydroxyacetophenone. An unusual oxygen-requiring reaction in the bacterial catabolism of 4-hydroxyacetophenone.
An enzyme, from a soil bacterium grown on 4-hydroxyacetophenone, cleaved the side chain of omega,4-dihydroxyacetophenone between the keto group and the carbon atom bearing a hydroxy group to give 4-hydroxybenzoate and formate. The reaction was O2-dependent. Partially purified enzyme required no added cofactors for activity.
D000098:Acetophenones / Q000378:metabolism*; D000421:Alcaligenes / Q000378:metabolism; D002848:Chromatography, DEAE-Cellulose; D005561:Formates / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D013053:Spectrophotometry
D000098:Acetophenones; D005561:Formates; C030544:formic acid; C031335:4-hydroxyacetophenone; D010100:Oxygen