Effects of methanol concentration and solvent:peanut ratio on extraction of aflatoxin from raw peanuts.
Aflatoxin B1 was extracted by a water slurry process using methanol concentrations of 55, 60, 65, and 70% in water and solvent:peanut ratios of 3, 4, 5, and 6 mL/g. Results failed to show that methanol concentration had an effect on amount of B1 extracted; however, the amount of B1 extracted increased with an increase in solvent:peanut ratio. Aflatoxin B1 was also extracted by the official AOAC method II, using methanol concentrations of 55, 60, 65, and 70% in water and solvent:peanut ratios of 2, 3, 4, and 5 mL/g. Results showed that the amount of B1 extracted increased with percent methanol at low solvent:peanut ratios but not at high ratios. Also, the amount of B1 extracted increased with solvent:peanut ratios at all methanol concentrations.
D000348:Aflatoxins / Q000302:isolation & purification*; D010367:Arachis / Q000032:analysis*; D000432:Methanol; D009821:Oils / Q000032:analysis; D012997:Solvents
D000348:Aflatoxins; D009821:Oils; D012997:Solvents; D000432:Methanol