Preparation of amniotic membranes for surgical use with antibiotic solutions.
A comparative study was conducted to investigate the effectiveness of antibiotic solutions for disinfecting vaginally delivered amniotic membranes. The microbial flora of eight amniotic membranes was defined and six antibiotic solutions were tested for their effectiveness in eliminating aerobic and anaerobic organisms. A solution containing 600 micrograms lincomycin HCl/ml, 500 micrograms neomycin sulfate/ml, 25 micrograms polymyxin B sulfate/ml, and 25 micrograms amphotericin B sulfate/ml in 0.5N saline was found to be capable of disinfecting amniotic membranes stored at 4 degrees C for 24 hours. This solution is now being used to disinfect surgically used amniotic membranes at our institution.
D000650:Amnion / Q000187:drug effects* / Q000382:microbiology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001684:Biological Dressings; D004203:Disinfection / Q000379:methods*; D005260:Female; D006801:Humans; D011247:Pregnancy; D013242:Sterilization / Q000379:methods*
D000900:Anti-Bacterial Agents