Improved survival and differentiation of newborn and adult mouse neurons in F12 defined medium by fibronectin.
Few newborn DRG neurons attached on polylysine coated coverslips in Ham's F12 medium, although most adult neurons attached on them. Fibronectin improved the attachment of newborn neurons and improved differentiation capacity of both neurons. Under this condition, newborn DRG neurons developed their processes more rapidly, but died more prematurely than adult neurons. Neural cell adhesion molecules and differentiation capacity of DRG neurons might change with aging.
D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005353:Fibronectins / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000502:physiology*
D005353:Fibronectins