title: Distribution of bone inductive proteins in mineralized and demineralized extracellular matrix.
abstract: Implantation of demineralized extracellular bone matrix results in new bone formation locally. Although the precise molecular mechanisms are not known, the reconstitution of matrix proteins less than 50,000 daltons with collagenous residue results in bone induction. The aim of the present investigation was to ascertain the distribution of the bone inductive protein(s) in various compartments of the tissue. A sequential extraction of mineralized bone matrix was employed: (1) 4 M guanidine HCl to extract proteins that are cell associated and not masked by mineral; (2) 0.5 M EDTA to dissolve the mineral phase; (3) 4 M guanidine HCl to reextract the collagenous matrix-associated proteins under dissociative conditions; (4) 4 M guanidine HCl containing 0.5 M EDTA to release any other residual proteins. This sequential method revealed that about 25% of total biological activity of bone induction is associated with first guanidine extraction, about 15% with the mineral phase and the rest of the activity is tightly associated with the collagenous matrix.
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001846:Bone Development; D001857:Bone Matrix / Q000502:physiology*; D019485:Bone Morphogenetic Proteins; D001842:Bone and Bones / Q000502:physiology*; D005269:Femur; D006133:Growth Substances / Q000302:isolation & purification* / Q000502:physiology; D011506:Proteins / Q000302:isolation & purification* / Q000502:physiology; D051381:Rats; D013977:Tibia
chemical_list: D019485:Bone Morphogenetic Proteins; D006133:Growth Substances; D011506:Proteins; D000469:Alkaline Phosphatase