Ready evaluation of lung alveolar toxic damage with histological sections morphometry.
In this experiment we aimed at developping a method of ready evaluation of lung alveolar toxic damage using morphometric measurements. Swiss-Webster mice were treated with different doses of diquat (i.p.) and the left lung was fixed at 0, 3, and 5 days after treatment. The technique of morphometry of histological sections embedded in glycol methacrylate (GMA) was used to quantify possible modifications of alveolar cells. Quantitative evaluation includes: alveolar macrophages, polymorphonuclear cells, type II and intermediate pneumocytes. The results indicate a lung dose-dependent response, observed in both inflammatory and epithelial regeneration components of the alveolar primary reaction. This method could be used to readily evaluate the pulmonary toxic potential of any substance.
D000818:Animals; D004178:Diquat / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology; D008171:Lung Diseases / Q000139:chemically induced / Q000473:pathology; D008297:Male; D051379:Mice; D011650:Pulmonary Alveoli / Q000187:drug effects* / Q000473:pathology
D004178:Diquat