Sensitive gas-liquid chromatographic procedure for urinary N tau-methylimidazole acetic acid, an index of histamine turnover.
A simple and specific gas-liquid chromatographic procedure, compatible with both nitrogen-phosphorous and electron-capture detection, and employing conventional packed columns, has been devised for urinary 1-methylimidazole-4-acetic acid (N tau-MeImAA), an index of whole-body histamine turnover. N tau-MeImAA is isolated by ion-exchange on Dowex 1 (CH3COO-), esterified by reaction with chloroethanol-boron trichloride and, depending upon detection employed, chromatographed on base-deactivated SP-2401 or SP-2250. [3H]N tau-MeImAA serves as internal recovery standard.
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D003404:Creatinine / Q000652:urine; D006632:Histamine / Q000378:metabolism*; D006801:Humans; D007093:Imidazoles / Q000652:urine*; D008297:Male; D051379:Mice
D007093:Imidazoles; C002667:methylimidazoleacetic acid; D006632:Histamine; D003404:Creatinine