title: Increased nuclear DNA content in raphide crystal idioblasts during development in Vanilla planifolia L. (Orchidaceae).
abstract: Longitudinal files of raphide crystal idioblasts form within the cortical meristematic region of Vanilla planifolia aerial roots. Cell and nuclear enlargement occur gradually throughout idioblast development and nuclear diameter approximates idioblast maturity. Cytophotometric determination of nuclear DNA (Feulgen) contents, measured by the two-wavelength method, revealed that all cortical parenchyma cells are diploid (2C = 6.3 pg), whereas all crystal idioblast nuclei are endopolyploid. Idioblast nuclear DNA content ranged from 4C to 32C (106 pg) and averaged 5.9 times that of parenchyma telophase nuclei. Frequency distribution of individual DNA content measurements depicts multiple genomes (increasing with geometric periodicity) to the 8C level, followed by less strict DNA replication within the crystal idioblast genome. The largest nuclei had the highest DNA content. Endomitotic stages of preprophasic heterochromatic dispersion (Z phase) and partial prophasic chromosomal coiling are observed with light and electron microscopy. DNA content values above the 8C level do not fit the geometrical order which is found if the total genome is replicated during each endo-cycle, a result indicating differential DNA replication. Chromocenter counts substantiate the occurrence of endomitosis to the 8C level and suggest heterochromatin underreplication in higher endopolyploid idioblast nuclei. Possible relationships between observed cytological events of idioblast development and nuclear condition are discussed.
mesh_terms: D002129:Calcium Oxalate; D002467:Cell Nucleus / Q000032:analysis*; D004247:DNA / Q000032:analysis*; D059828:Plant Cells; D010944:Plants / Q000032:analysis / Q000378:metabolism*
chemical_list: D002129:Calcium Oxalate; D004247:DNA