Simultaneous determination of chloroprocaine hydrochloride and its degradation product 4-amino-2-chlorobenzoic acid in bulk drug and injection solutions by high-performance liquid chromatography.
A high-performance liquid chromatographic method has been developed for the simultaneous determination of chloroprocaine hydrochloride and its hydrolytic degradation product, 4-amino-2-chlorobenzoic acid. Separation is achieved using a mu-Bondapak C18 column and the eluant, water-acetonitrile-methanol-glacial acetic acid (74:20:5:1) containing 0.05-0.08% (w/v) sodium 1-heptanesulfonate, at a flow rate of 2 mL/min. Benzoic acid and p-nitroacetophenone were used as internal standards. A variable-wavelength UV detector (278 nm) was used for detection of the compounds. The method is simple, accurate, and precise.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006868:Hydrolysis; D011343:Procaine / Q000031:analogs & derivatives* / Q000032:analysis; D012996:Solutions / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives* / Q000032:analysis
D012996:Solutions; D011343:Procaine; D005680:gamma-Aminobutyric Acid; C004616:chloroprocaine; C029210:2-chloro-4-aminobenzoic acid