A rapid method for the determination of antibody affinity.
A rapid method for estimating antibody affinity is described. To perform the assay fixed amounts of radiolabeled antigen and antibody are mixed in different total reaction volumes. The fraction of antigen bound to antibody is measured in aliquots from each reaction volume by the ammonium sulfate precipitation technique. Affinity constants can be estimated from these data graphically or by means of a microcomputer. This method is much quicker and easier to perform than traditional techniques for estimating antibody affinity.
D000818:Animals; D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D002417:Cattle; D008722:Methods; D011817:Rabbits