A new reaction useful for chemical cross-linking between nucleic acids and proteins.
Cytosine in nucleic acids can be converted into N4-aminocytosine by treatment with a mixture of hydrazine and bisulfite. The hydrazino group thus formed at position 4 of the pyrimidine ring can be linked to a sulhydryl group in proteins by the use of bromopyruvate as a linker. Successful use of this scheme of chemical cross-linking between nucleic acid and protein was demonstrated in the linking of poly(C) with glutathione, and of RNA with protein in the E. coli 30 S ribosomal subunit.
D003432:Cross-Linking Reagents; D005978:Glutathione; D006834:Hydrazines; D009696:Nucleic Acids; D011506:Proteins; D011773:Pyruvates; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D013447:Sulfites
D003432:Cross-Linking Reagents; D006834:Hydrazines; D009696:Nucleic Acids; D011506:Proteins; D011773:Pyruvates; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D013447:Sulfites; C017092:bromopyruvate; D005978:Glutathione