Location of a binding site for 1-anilinonaphthalene-8-sulfonate on calmodulin.
The quenching by radiationless energy transfer of the ultraviolet fluorescence of Tyr-99 and Tyr-138 by bound 1-anilinonaphthalene-8-sulfonate (1,8-ANS) has been employed to determine the separation of a hydrophobic binding site of 1,8-ANS from each of the tyrosines. The results suggest that the dominant binding site is located in the N-terminal region of domain III.
D000817:Anilino Naphthalenesulfonates / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002147:Calmodulin / Q000378:metabolism*; D002417:Cattle; D005456:Fluorescent Dyes; D008297:Male; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D014443:Tyrosine
D000817:Anilino Naphthalenesulfonates; D002147:Calmodulin; D005456:Fluorescent Dyes; D014443:Tyrosine; C027132:1-anilino-8-naphthalenesulfonate