Rapid freezing and frozen sectioning as a means of preserving brain vessel morphometric characteristics.
A study was performed for the purpose of determining whether ordinary frozen sections of brain could preserve in vivo parenchymal arteriole morphometric characteristics. The results showed that flash-freezing followed by frozen sectioning is just as reliable a means of preserving brain arteriole morphometric characteristics as the time consuming process of flash-freezing followed by freeze substitution.
D000818:Animals; D001160:Arterioles / Q000033:anatomy & histology; D002536:Cerebral Arteries / Q000033:anatomy & histology*; D005615:Freezing; D005629:Frozen Sections; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014021:Tissue Preservation / Q000379:methods*