Preparation of a branched heptasaccharide by bacteriophage depolymerization of Klebsiella K60 capsular polysaccharide.
Bacteriophage phi 60 possesses an endoglucosidase that depolymerizes the capsular polysaccharide of Klebsiella K60 into a heptasaccharide having two single beta-D-glucopyranosyl side-chains. This bulky oligosaccharide may be used as a probe to examine the combining sites of immunoglobulins. The 1H- and 13C-n.m.r.-spectral data of the oligosaccharide are reported.
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D007709:Klebsiella / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D009844:Oligosaccharides / Q000302:isolation & purification*; D009904:Optical Rotation; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification*
D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial