Interaction between phosphatidylserine vesicles and rat brain synaptosomes.
Intact rat brain synaptosomes incubated with phosphatidylserine vesicles incorporated a large amount of the phospholipid at membrane level. Chromatographic analysis showed that phosphatidylserine was not modified after the interaction. Ca2+ and an hypertonic medium favored the interaction between vesicles and synaptosomes. The phenothiazine fluphenazine blocked this effect. We suggest that the interaction of phosphatidylserine with synaptosomes could occur as a consequence of a two-step process: an initial non specific binding of the phospholipid to the membrane; a specific incorporation, via fusion, mediated by microM Ca2+ and by hypertonicity of the medium and regulated by calmodulin or calmodulin-like proteins.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D005476:Fluphenazine / Q000494:pharmacology; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism*; D009994:Osmolar Concentration; D010718:Phosphatidylserines / Q000378:metabolism*; D051381:Rats; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*
D008081:Liposomes; D010718:Phosphatidylserines; D005476:Fluphenazine; D002118:Calcium