Tween-80 alters natural resistance of sarcoma-180 to bouvardin (NSC 259968).
Sarcoma-180 tumour (S-180) exhibits natural resistance to bouvardin (NSC 259968), a protein synthesis inhibitor that also inhibits RNA and DNA synthesis when administered over a range of non-toxic doses using 0.9% NaCl as a vehicle. However, using Tween-80 as a vehicle, there is a substantial enhancement of cytotoxicity and a subsequent increase in the life span of animals bearing the S-180 tumour. This observation was substantiated in vitro by exposing S-180 cells to 10(-6)M bouvardin in the presence and absence of Tween-80. Bouvardin, 10(-6)M, in the absence of Tween-80, inhibited the incorporation of [3H]uridine by 46%, whereas the presence of Tween-80 resulted in a 66% inhibition of uridine incorporation.
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002460:Cell Line; D003864:Depression, Chemical; D004351:Drug Resistance; D004357:Drug Synergism; D051379:Mice; D009368:Neoplasm Transplantation; D010456:Peptides, Cyclic / Q000494:pharmacology*; D011136:Polysorbates / Q000494:pharmacology*; D012513:Sarcoma, Experimental / Q000378:metabolism*; D012965:Sodium Chloride; D012996:Solutions; D013997:Time Factors; D014529:Uridine / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D010456:Peptides, Cyclic; D011136:Polysorbates; D012996:Solutions; C018234:bouvardin; D012965:Sodium Chloride; D014529:Uridine