Influence of diet on de novo lipogenesis in artificially-fed 15- and 20-day-old Zucker fatty rats.
Lean and obese Zucker rat pups were artificially fed high-fat and high-carbohydrate diets from 10-20 d of age to determine the effect of early diet composition on de novo lipogenesis and the development of obesity in the Zucker fatty rat. Amounts fed were the same as that consumed by suckling Zucker rat pups, which also were used as dam-fed controls. Lipogenic rates were measured by in vivo incorporation of tritium from 3H2O into total lipids and fatty acid and glyceride-glycerol fractions of liver, carcass and adipose tissue. Expressed on a per g tissue basis there was no difference in lipogenic rates between lean and obese pups in any tissues, even though pups accumulated a significantly higher percentage of carcass fat. High carbohydrate feeding accelerated growth and lipogenic rates in both lean and obese rats. Dam-fed pups had the lowest rates of lipogenesis and percentage of carcass fat. Thus, diet composition and method of feeding were important determinants of de novo lipogenic rates and body energy stores at this early age, but their influence was similar in lean and genetically obese animals.
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D004040:Dietary Carbohydrates / Q000009:adverse effects*; D004041:Dietary Fats / Q000009:adverse effects*; D005227:Fatty Acids / Q000378:metabolism; D005989:Glycerides / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006614:Hindlimb; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D009765:Obesity / Q000209:etiology*; D051381:Rats; D011924:Rats, Zucker
D004040:Dietary Carbohydrates; D004041:Dietary Fats; D005227:Fatty Acids; D005989:Glycerides; D005990:Glycerol