Evaluation of a direct solid-phase radioimmunoassay for progesterone, useful for monitoring luteal function.
We have evaluated a commercially available, direct, solid-phase radioimmunoassay kit for progesterone determination in serum or plasma. The assay is precise, within-run precision (CV) in the clinically significant ranges being 2.5 to 5.2%, between-run 5.5 to 5.8%. Mean analytical recovery of different concentrations of progesterone added to serum was 99.7% (range 95.3 to 102.7%). Fourteen closely related steroids showed no cross reactivity. The minimum detection limit was 0.5 microgram/L. Luteal-phase progesterone concentrations in serum were increased (greater than 3 micrograms/L) in 19 normal ovulatory menstrual cycles and decreased (less than 1.5 micrograms/L) in two nonovulatory cycles. We found this direct assay for progesterone to be analytically and clinically sound, and useful for assessing luteal-phase function.
D003338:Corpus Luteum / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008183:Luteal Phase; D008297:Male; D011374:Progesterone / Q000097:blood*; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic; D013223:Statistics as Topic
D011933:Reagent Kits, Diagnostic; D011374:Progesterone