title: Isolation and characterization of four adenovirus type 12-transformed human embryo kidney cell lines.
abstract: Four transformed cell lines were established from cultures of human embryo kidney (HEK) cells microinjected or transfected with cloned adenovirus 12 (Ad12) EcoRI-C DNA (0 through 16.5 map units of the left-hand end of the viral genome). Each cell line showed a different growth pattern. Southern blotting demonstrated that all of the cell lines contained Ad12-specific DNA sequences, but in the microinjected isolates these were at a much lower copy number than in the transfected isolate. Two cell lines (Ad12 HEK 1 and 3) appeared to contain tandemly repeated Ad12 EcoRI-C DNA fragments. Immunoprecipitation and Western blotting confirmed that Ad12 early region 1 (E1) proteins were being expressed by all four of the transformed cell lines, but indicated that E1A polypeptide expression was considerably less than E1B polypeptide expression. All of the Ad12-transformed HEK cell lines were tumorigenic when inoculated intracranially into athymic nude mice.
mesh_terms: D000260:Adenoviruses, Human; D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D002460:Cell Line; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000302:isolation & purification; D004622:Embryo, Mammalian; D006801:Humans; D007668:Kidney; D051379:Mice; D009374:Neoplasms, Experimental / Q000209:etiology; D014764:Viral Proteins / Q000302:isolation & purification
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins