Granulocytes are more sensitive than lymphocytes to changes in EA and EAC indicator cell quality.
Granulocytes were more sensitive to age variation of EAC3b indicator cells than lymphocytes, and showed a significant decrease in rosette formation with EAC3b indicator cells more than 2 weeks old; whereas lymphocytes showed unchanged EAC3b rosette formation with indicator cells up to 4 weeks old. EAIgG rosette formation by granulocytes was less affected by the age of the indicator cells than by their degree of sensitization, with a 25% loss of rosettes after 1 dilution of the sensitizing antibody. Thus optimal sensitization is essential for granulocyte EAIgG rosette formation, and relatively fresh indicator cells for EAC3b rosette formation. Hemolysis of contaminating erythrocytes did not influence EAIgG or EAC3b rosette formation.
D003165:Complement System Proteins / Q000378:metabolism; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000276:immunology* / Q000502:physiology; D006098:Granulocytes / Q000276:immunology*; D006384:Hemagglutination; D006461:Hemolysis; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D008214:Lymphocytes / Q000276:immunology*; D012397:Rosette Formation / Q000379:methods*
D007074:Immunoglobulin G; D003165:Complement System Proteins