A combined flat-embedding, HRP histochemical method for correlative light and electron microscopic study of single neurons.
A simple and reproducible method is described for studying the morphology of the same neuron at the light and electron microscopic level. This method utilizes horseradish peroxidase histochemistry and a recently described flat-embedding procedure wherein thin, aldehyde-fixed sections are placed in resin between glass microscope slides pretreated with dimethyldichlorosilane. The significance of these combined methodologies for correlative light and electron microscopic studies of single neurons is discussed.
D000818:Animals; D001560:Benzidines; D005260:Female; D006651:Histocytochemistry / Q000379:methods*; D006735:Horseradish Peroxidase; D008853:Microscopy / Q000379:methods; D008854:Microscopy, Electron; D009046:Motor Neurons / Q000648:ultrastructure; D009474:Neurons / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains; D012821:Silanes
D001560:Benzidines; D012821:Silanes; C021758:3,3',5,5'-tetramethylbenzidine; C040863:dichlorodimethylsilane; D006735:Horseradish Peroxidase