[Isolation and use of stable borate-polyol pH gradients in the range of 1.9-12.0 for isoelectric focusing of proteins and low-molecular compounds].
Compositions of borate-polyol buffer solutions were developed, which enabled to produce pH gradients stable to the effect of electric potential in the limits of pH 1.9-4.5, pH 9.6-12.0 and pH 2.0-10.0. These buffers were used for isoelectrofocusing of proteins, low molecular peptides and amino acids.
D001881:Borates / Q000138:chemical synthesis / Q000302:isolation & purification*; D002021:Buffers; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D011108:Polymers / Q000138:chemical synthesis / Q000302:isolation & purification*
D001881:Borates; D002021:Buffers; D011108:Polymers; C024617:polyol
Poluchenie i ispol'zovanie stabil'nykh borat-poliol'nykh gradientov pH v diapazone 1,9-12,0 dlia izoélektricheskogo fokusirovaniia belkov i nizkomolekuliarnykh soedineniĭ.