A rapid procedure for screening and isolating oxygenated free fatty acid metabolites from plants and natural waters.
A rapid method of isolating a relatively pure fraction of oxygenated fatty acids from plants and natural waters is described. These metabolites were isolated from aqueous extracts using octadecylsilyl silica in a reverse-phase batch extraction method. The extraction method, together with reverse-phase analytical high pressure liquid chromatography (HPLC), was used to establish a routine screening method for the presence of these compounds in a variety of natural sources. A reverse-phase preparative HPLC purification method is also described.
D002851:Chromatography, High Pressure Liquid; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D010084:Oxidation-Reduction; D010944:Plants / Q000032:analysis*; D014867:Water / Q000032:analysis*
D005230:Fatty Acids, Nonesterified; D014867:Water