Immunochemical characterization of a monoclonal anti-Leb blood grouping reagent.
A haemagglutinating monoclonal anti-LebL antibody has been obtained from a mouse-mouse hybridoma after immunization with the Leb-active oligosaccharide lacto-N-difucohexaose I, coupled to edestin. Of 13 other antibodies obtained, one was able to agglutinate enzyme-treated erythrocytes of all ABO groups and three more were considered to be anti-LebH haemagglutinins. The remaining nine antibodies did not agglutinate red cells. The specificity of the stronger of the anti-LebL haemagglutinins was examined by studying its binding to a number of glycoconjugates and oligosaccharides.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D005260:Female; D006386:Hemagglutination Tests; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007202:Indicators and Reagents / Q000032:analysis; D007518:Isoantibodies / Q000096:biosynthesis / Q000276:immunology*; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D007518:Isoantibodies; D007983:Lewis Blood Group Antigens