Catechol-O-methyltransferase: a method for autoradiographic visualization of isozymes in cellogel.
An electrophoretic procedure for separating the molecular forms of catechol-O-methyltransferase in cellulose acetate gel is described; the zones of enzyme activity were revealed by autoradiography. The electrophoretic patterns of the enzyme in several tissues and cell lines derived from four different species are presented.
D000818:Animals; D001345:Autoradiography; D002394:Catechol O-Methyltransferase / Q000302:isolation & purification* / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D051381:Rats; D011916:Rats, Inbred F344; D012436:S-Adenosylmethionine / Q000378:metabolism; D012867:Skin / Q000201:enzymology; D013462:Sulfur Radioisotopes
D007527:Isoenzymes; D013462:Sulfur Radioisotopes; D012436:S-Adenosylmethionine; D002394:Catechol O-Methyltransferase