title: Effects of temperature on the frequency of sister chromatid exchanges (SCEs) in peripheral blood lymphocytes of man and muntjac.
abstract: The incidence of sister chromatid exchanges (SCEs) and cell proliferation kinetics have been studied in peripheral blood lymphocytes of man and muntjac grown at 33 to 44 degrees C to gain insight into SCE formation. The frequency of SCEs increased as a function of growth temperature. At a given temperature, however, the frequency of SCEs varied with the sampling times; the early sampled cells showed fewer SCEs than did those harvested late. At 33 degrees C the frequency of SCEs was lowest and there was a marked delay in cell-cycle progression. The number of SCEs was maximum at 40 degrees C in human and 42 degrees C in muntjac. Cell proliferation was markedly affected at higher temperature and 44 degrees C was found to be intolerable for lymphocytes of both the species. It is proposed that certain temperature-dependent enzyme(s) associated with DNA replication kinetics may be involved in the formation of SCEs.
mesh_terms: D000818:Animals; D002453:Cell Cycle; D003434:Crossing Over, Genetic; D004261:DNA Replication; D003670:Deer; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure*; D008297:Male; D012854:Sister Chromatid Exchange; D013696:Temperature