Demonstration of histamine receptors on human platelets by flow cytometry.
Fluoresceinated human albumin conjugated with histamine (FHA-HIS) has been used for the demonstration of histamine receptors on human platelets. Such receptors were demonstrated on 40-63% of peripheral blood platelets in 4 healthy donors. The binding of FHA-HIS was inhibited on 35-79% of the platelets by the histamine H1 receptor antagonists diphenhydramine and clemastine. The histamine H2 receptor antagonist cimetidine blocked the FHA-HIS binding on 14-37% of the platelets. It is concluded that histamine H1 as well as H2 receptors occur on human platelets but the receptors are not equally distributed in the platelet population.
D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects; D002927:Cimetidine / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D011968:Receptors, Histamine / Q000032:analysis*; D011969:Receptors, Histamine H1 / Q000032:analysis*; D011970:Receptors, Histamine H2 / Q000032:analysis*
D011968:Receptors, Histamine; D011969:Receptors, Histamine H1; D011970:Receptors, Histamine H2; D002927:Cimetidine