Development of a GC-MS method for quantitation of 2,3-dinor-TxB2 and determinations of the daily urinary excretion rates in healthy humans.
Tetradeuterated 2,3-dinor-thromboxane B2 (2,3-dinor-TxB2) of high isotopic purity was prepared. This compound was used as internal standard in the development of a method for quantitation of 2,3-dinor-TxB2 in human urine based on gas chromatography--mass spectrometry with multiple ion detection. The accuracy of the method is about +/- 5% at the levels encountered when urine from normal healthy individuals was analyzed. The daily urinary excretion of 2,3-dinor-TxB2 in twenty normal healthy males and females was 457 +/- 210 (mean +/- SD) and 444 +/- 160 (mean +/- SD) ng/24 hrs respectively. The diurnal and day-to-day variations were found to be relatively small.
D000328:Adult; D002648:Child; D002675:Child, Preschool; D002940:Circadian Rhythm; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D012016:Reference Values; D013929:Thromboxane B2 / Q000031:analogs & derivatives / Q000652:urine*; D013931:Thromboxanes / Q000652:urine*
D013931:Thromboxanes; D013929:Thromboxane B2; C028965:2,3-dinor-thromboxane B2