Detection of Clostridium difficile in faeces by direct gas liquid chromatography.
Stool specimens examined for the presence of Clostridium difficile and its cytotoxin were screened by gas liquid chromatography for the presence of volatile fatty acids and p-cresol. Twenty seven of 110 (25%) stools yielded C difficile or cytotoxin; iso-valeric acid was detected in 63/110 (57%) and iso-caproic acid in 18/110 (16%) stools. Para-cresol was found in 24/71 (34%) stools examined. Iso-valeric acid was detected in 85% of stools positive for C difficile, whereas iso-caproic acid (41%) and p-cresol (52%) were found in much lower numbers of C difficile-positive stools. It is concluded that gas chromatographic detection of volatile fatty acids or p-cresol in faeces are not satisfactory screening tests for the presence of C difficile.
D002208:Caproates / Q000032:analysis; D002849:Chromatography, Gas; D003013:Clostridium / Q000302:isolation & purification*; D003408:Cresols / Q000032:analysis; D003603:Cytotoxins / Q000032:analysis; D005232:Fatty Acids, Volatile / Q000032:analysis; D005243:Feces / Q000032:analysis / Q000382:microbiology*; D045782:Hemiterpenes; D006801:Humans; D010421:Pentanoic Acids / Q000032:analysis
D002208:Caproates; D003408:Cresols; D003603:Cytotoxins; D005232:Fatty Acids, Volatile; D045782:Hemiterpenes; D010421:Pentanoic Acids; C008216:isovaleric acid; C032538:4-cresol; C034527:isocaproic acid