The biochemical and functional heterogeneity of circulating human plasma fibronectin.
Human plasma fibronectin purified by affinity chromatography, consisted of homogeneous 215 kD protein subunits when assessed by SDS polyacrylamide gel electrophoresis. On isoelectric focusing however, 5 separate fractions were present, with isoelectric points ranging from 5.6 to 6.1. Isoelectric focusing and immunofixation of native plasma produced similar but not identical appearances. Only 15% of the total plasma fibronectin opsonically stimulated the ingestion of Saccharomyces cerevisiae by human peripheral blood monocytes, and this opsonic fibronectin was confined to the fraction with an isoelectric point of 6.1.
D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D007525:Isoelectric Focusing; D008970:Molecular Weight
D005353:Fibronectins