Sodium dodecyl sulfate polyacrylamide gel electrophoresis of serum after protein denaturation in the presence or absence of 2-mercaptoethanol.
Human serum proteins were evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis after protein denaturation in the presence or absence of 2-mercaptoethanol. Both electrophoretic techniques give characteristic and reproducible banding patterns and achieve a high degree of resolution within the limits of a one-dimensional separation. The major protein bands have been identified, and the merits of the two techniques are compared. Protein denaturation in the absence of 2-mercaptoethanol gives more discrete bands for the purpose of protein identification by maintaining the disulfide-bridge-dependent polypeptide associations characteristic of many serum proteins. However, simultaneous use of both electrophoretic techniques enhances identification by exploiting the response of an individual protein to mercaptoethanol treatment. The clinical potential of this approach for detecting protein disorders is discussed.
D000818:Animals; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D007070:Immunoglobulin A / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D007202:Indicators and Reagents; D008623:Mercaptoethanol; D009101:Multiple Myeloma / Q000097:blood; D011489:Protein Denaturation; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate
D001798:Blood Proteins; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D007202:Indicators and Reagents; D012967:Sodium Dodecyl Sulfate; D008623:Mercaptoethanol