Rapid semiquantitative measurement of total porphyrins in urine and feces by magnetic circular dichroism.
Magnetic circular dichroism is a suitable technique for semiquantitative determination of total porphyrins in urine and feces, being rapid, reliable, and involving little sample preparation. The sensitivity of the assay suffices to distinguish normal from above-normal concentrations of total porphyrins in urine and feces. Results for urine compare well with those obtained by thin-layer chromatography of porphyrin esters (n = 42, r = 0.92) or ion-exchange column chromatography of porphyrin acids (n = 19, r = 0.80).
D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D002942:Circular Dichroism; D003306:Coproporphyrins / Q000032:analysis; D005243:Feces / Q000032:analysis*; D006801:Humans; D011164:Porphyrias / Q000378:metabolism*; D011166:Porphyrins / Q000032:analysis / Q000652:urine*; D011524:Protoporphyrins / Q000032:analysis; D013057:Spectrum Analysis; D014578:Uroporphyrins / Q000032:analysis
D003306:Coproporphyrins; D011166:Porphyrins; D011524:Protoporphyrins; D014578:Uroporphyrins