[Use of high performance liquid chromatography of methylbenzocoumarin esters of prostaglandins in their quantitative fluorimetric analysis].
A method is suggested for quantitative fluorimetric analysis of natural prostaglandins (PGs). It is based on preparation of the fluorescent methylbenzocoumarine PG esters after their reaction with 4-bromomethyl-7.8-benzocoumarine (Br-MBC). Conditions for the reverse-phase high-performance liquid chromatography were evaluated, which permitted separating PGs into groups and according to the degree of unsaturation. Sensitivity and linear dependence of the ester fluorescence on the PG concentration make this method suitable for the quantitative analysis of PGs in different biological objects.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer; D011453:Prostaglandins / Q000032:analysis*
D011453:Prostaglandins
Primenenie vysokoéffektivnoĭ zhidkostnoĭ khromatografii metilbenzkumarinovykh éfirov prostaglandinov dlia ikh kolichestvennogo fluorimetricheskogo analiza.