Effects of adrenalectomy and adrenal steroids on the uptake of serotonin in rat platelets.
Changes induced by adrenalectomy, triamcinolone treatment and deoxycorticosterone (DOC) treatment on the uptake of [3H]-serotonin into platelets were studied ex vivo, using rat platelets suspended in physiological medium. Adrenalectomy caused a decrease in active uptake of serotonin and an associated increase in apparent Km. Treatment of the adrenalectomized rats with triamcinolone (0.25 mg/kg), but not DOC (1.0 mg/kg) restored the active uptake of serotonin. At triamcinolone dose of 0.5 mg/kg, the serotonin uptake was raised to a level that was significantly higher than normal. Kinetic analysis of uptake data showed the apparent Km to be affected by both triamcinolone and DOC, while the apparent Vmax of uptake rate was increased by triamcinolone treatment.
D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D000315:Adrenalectomy; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D003900:Desoxycorticosterone / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D010976:Platelet Count; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000097:blood*; D014221:Triamcinolone / Q000494:pharmacology
D000305:Adrenal Cortex Hormones; D014221:Triamcinolone; D012701:Serotonin; D003900:Desoxycorticosterone