Laminin promotes attachment and neurite elongation of fetal hypothalamic neurons grown in serum-free medium.
Laminin, as a coating or in solution, allows a rapid attachment of fetal hypothalamic cells in serum-free medium, and strikingly enhances the neurite network development. As compared to cultures grown on a fetal calf serum coating, cells remain in clusters and astrocytes become fibrous. Laminin was visualized by immunocytochemistry in non-neuronal cells. The number of laminin-positive cells was lower in cultures grown in serum-free medium than in those grown in serum-supplemented medium. In both culture conditions, their number decreases with time in vitro.
D000818:Animals; D001253:Astrocytes / Q000166:cytology; D001485:Basement Membrane / Q000502:physiology; D002448:Cell Adhesion; D002478:Cells, Cultured; D003470:Culture Media; D007031:Hypothalamus / Q000166:cytology*; D007797:Laminin / Q000502:physiology*; D051379:Mice; D009474:Neurons / Q000166:cytology
D003470:Culture Media; D007797:Laminin