Factors influencing the accumulation of tetraphenylphosphonium cation in HeLa cells.
Exposure of HeLa cells to tetraphenylphosphonium cation (TPP+) results in a rapid accumulation intracellularly, and a steady-state level is reached within 10 min. Accumulation of [3H]TPP+ in HeLa cells is reduced under the following conditions: (i) after preincubation of cells in buffered saline or in medium containing two- to fourfold higher concentrations of amino acids, (ii) exposure to the alkylating agent L-1-tosylamido-2-phenyl-ethylchloromethyl ketone, (iii) ouabain-mediated inhibition of the Na+, K+ ATPase, and (iv) high external K+ concentrations. In contrast, addition of serum increases the uptake of TPP+. In synchronized cells, intracellular levels of TPP+ differ at various stages of cell cycle and are lowest in mitosis.
D001693:Biological Transport, Active / Q000187:drug effects; D002453:Cell Cycle; D003470:Culture Media; D006367:HeLa Cells / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008564:Membrane Potentials; D009861:Onium Compounds / Q000378:metabolism*; D009943:Organophosphorus Compounds; D014108:Tosylphenylalanyl Chloromethyl Ketone / Q000494:pharmacology
D003470:Culture Media; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D014108:Tosylphenylalanyl Chloromethyl Ketone; C013289:tetraphenylphosphonium