title: Measurement of collagen-fibre diameter in human skin.
abstract: A method for the measurement of collagen fibre diameter in human skin is described. Photographic reduction of electron micrograph negatives onto positive transparency film allows the use of a Quantimet 720 image analysis system for relatively rapid determination of mean collagen fibre diameter based upon the mean cross-sectional area of a single electron microscope field. The mean collagen fibre diameter from papillary and reticular dermis in normal skin was 56.2 +/- 2.5 nm and 62.8 +/- 4.3 nm respectively, and exhibited slightly skewed unimodal distributions.
mesh_terms: D000328:Adult; D002990:Clobetasol / Q000031:analogs & derivatives / Q000494:pharmacology; D003094:Collagen; D003201:Computers; D006801:Humans; D008854:Microscopy, Electron / Q000379:methods; D010781:Photography; D012016:Reference Values; D012867:Skin / Q000187:drug effects / Q000648:ultrastructure*
chemical_list: D003094:Collagen; D002990:Clobetasol