Large scale preparation of Rhizobium meliloti bacteriophages by fermenter culture.
A simple method for preparation of Rhizobium meliloti bacteriophages was established employing fermenter culture. This technique allowed phage production to be checked by dissolved oxygen measure. Phage suspensions ranging from 5.10(12) to 1.2.10(13) PFU/ml were found after polyethylene glycol precipitation and centrifugation in CsCl.
D001433:Bacteriolysis; D001435:Bacteriophages / Q000254:growth & development* / Q000302:isolation & purification; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D005285:Fermentation; D011092:Polyethylene Glycols; D012231:Rhizobium / Q000254:growth & development; D010948:Viral Plaque Assay; D014776:Virus Cultivation / Q000379:methods*
D011092:Polyethylene Glycols