Effect of E-64, a thiol protease inhibitor, on antibody formation in mice.
E-64, L-trans-epoxysuccinyl-leucylamido (4-guanidino) butane, a specific inhibitor of thiol proteases originally isolated from a culture broth of fungi, and its synthetic analogues, were examined for immune responses to the splenocytes of BDF1 mice. In the cultures of 2-day-primed splenocytes of the mice, E-64 and its close analogues, increased the number of direct splenic hemolytic plaque forming cells (PFC). In addition, it was demonstrated that E-64 enhanced the PFC responses in the mice. These results suggested that some thiol proteases might be involved in the immune response process in mice.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011480:Protease Inhibitors / Q000494:pharmacology*; D012756:Sheep; D013329:Structure-Activity Relationship; D013997:Time Factors
D011480:Protease Inhibitors; D007930:Leucine; C024974:E 64