3-(Trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine, a hydrophobic, photoreactive probe, labels calmodulin and calmodulin fragments in a Ca2+-dependent way.
3-(Trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine [( 125I]TID), a highly hydrophobic, carbene-generating photoreactive probe, labels calmodulin and some of its proteolytic fragments in the Ca2+-bound conformation only. It is assumed that [125I]TID labels hydrophobic sites exposed by the binding of Ca2+. The finding offers a new and powerful means to characterize calmodulin sites that play a role in the interaction with targets.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001389:Azirines / Q000494:pharmacology*; D001921:Brain; D002118:Calcium / Q000494:pharmacology*; D002147:Calmodulin / Q000378:metabolism*; D002417:Cattle; D007457:Iodine Radioisotopes; D007700:Kinetics; D010446:Peptide Fragments / Q000378:metabolism; D010777:Photochemistry
D000596:Amino Acids; D001389:Azirines; D002147:Calmodulin; D007457:Iodine Radioisotopes; D010446:Peptide Fragments; C033174:3-(trifluoromethyl)-3-(3-iodophenyl)diazirine; D002118:Calcium