Crystallization and preliminary X-ray study of a complex between d(ATGCAT) and actinomycin D.
Crystals of a 2:1 complex between the self-complementary DNA hexamer d(ATGCAT) and the antitumor drug actinomycin D have been grown from solutions of polyethylene glycol 400. The crystals are orthorhombic with space group P2(1)2(1)2(1) and a = 95.6, b = 42.7, and c = 40.8 A. A Patterson map calculated from preliminary diffractometer data as well as packing considerations suggest a model in which the actinomycin D is intercalated into a double-stranded DNA hexamer. There are four such complexes in the asymmetric unit.
D003460:Crystallization; D003609:Dactinomycin; D007364:Intercalating Agents; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D014961:X-Ray Diffraction
D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D003609:Dactinomycin