title: The preservation of the ability of cultured quail wing bud mesoderm to elicit a position-related differentiative response.
abstract: A previous study showed that grafting wedges of fresh anterior quail wing mesoderm into posterior slits of chick wing buds resulted in the formation of rods and nodules of cartilage in a high percentage of cases (B. Carlson, 1983, Dev. Biol. 101, 97-105). The purpose of the present study was to determine if a similar response could be elicited by grafting pieces of mesoderm that had been cultured in vitro. When pieces of 1-day cultured anterior mesoderm from stage 17-24 donors were grafted into standard posterior slits of chick wing buds, the percentages of supernumerary structures differed little from those which formed after the grafting of pieces of fresh mesoderm. In a time series, grafts of stage 22-23 anterior mesoderm which had been cultured for 1-4 days retained the ability to form cartilage after being grafted into posterior locations. A time series showed that the duration of this retention was longer in cultured mesoderm than it was in mesoderm that remains in the donor wing bud.
mesh_terms: D000367:Age Factors; D000818:Animals; D001842:Bone and Bones / Q000196:embryology; D002356:Cartilage / Q000196:embryology; D002642:Chick Embryo; D003370:Coturnix; D046508:Culture Techniques; D004477:Ectogenesis; D004627:Embryonic Induction; D008648:Mesoderm / Q000166:cytology; D009024:Morphogenesis; D013997:Time Factors; D014921:Wings, Animal / Q000196:embryology* / Q000637:transplantation