Assay for beta-glucuronidase utilizing headspace gas chromatography.
An assay for beta-glucuronidase is described. The assay uses 1-(beta-D-glucopyranuronosyl)pyridinium hydroxide, inner salt, as substrate and the quantitative determination is performed with headspace gas chromatography, measuring one of the products, pyridine, in the gas phase. The assay has been developed utilizing commercially available beta-glucuronidase (EC 3.2.1.31.) from Escherichia coli, and has been applied to various sources of beta-glucuronidase. Crude homogenates can be assayed directly with a minimum of manipulative steps and the method is suited for automation.
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D005243:Feces / Q000032:analysis; D005966:Glucuronidase / Q000032:analysis*; D006801:Humans; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology; D051379:Mice; D008819:Mice, Nude; D009132:Muscles / Q000201:enzymology; D009368:Neoplasm Transplantation; D051381:Rats; D011919:Rats, Inbred Strains
D005966:Glucuronidase