An analysis of repeated sequence heterogeneity.
High-resolution thermal denaturation was used to measure the heterogeneity within repeated DNA sequences. An analysis of combined denaturation/redenaturation experiments on mouse satellite DNA suggests the existence of two minor components, one of which does not appear in the prepared EcoRII monomer. The resolving power of the denaturation/redenaturation experiment is estimated and contrasted with that of the reassociation experiment, often used to estimate repeated sequence heterogeneity. A mathematical model of the redenaturation experiment was developed and applied to mouse satellite data; the results suggest that only one-fourth of the mismatched base pairs are energetically significant in the reduction of heteroduplex stability.
D000818:Animals; D004247:DNA / Q000235:genetics*; D004276:DNA, Satellite; D004279:DNA, Viral; D006358:Hot Temperature; D007700:Kinetics; D008099:Liver; D051379:Mice; D008809:Mice, Inbred C3H; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid
D004276:DNA, Satellite; D004279:DNA, Viral; D004247:DNA