title: Intermediates in 16-androstene biosynthesis in neonatal porcine testis.
abstract: The metabolism of pregnenolone has been studied in microsomal preparations of testes from 3-week old piglets. Metabolites identified after derivatization by capillary gas-chromatography and further by combined gas chromatography-mass spectrometry were: 17-hydroxy- and 16 alpha-hydroxypregnenolone, dehydroepiandrosterone, 5-androstenediols, 5-pregnenediol and andien-beta. Pregnenolone and 16-dehydro-pregnenolone, previously not separable by other methods, were successfully resolved as methyl oximetrimethylsilyl ethers using capillary column gas-chromatography.
mesh_terms: D000736:Androstenes / Q000096:biosynthesis*; D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D008861:Microsomes / Q000378:metabolism; D011284:Pregnenolone / Q000378:metabolism; D013552:Swine; D013737:Testis / Q000378:metabolism*
chemical_list: D000736:Androstenes; D011284:Pregnenolone