[Preparative isolation of hexa-, hepta-, octa-, nona- and decapyrimidine oligonucleotides from hydrolysates of depurinated herring sperm DNA].
The pyrimidine oligonucleotides (dT)5; (dT)6 and the mixtures of sequence isomers (dC, dT5); (dC2, dT5); (dC3, dT4); (dC4, dT3); (dC4, dT4); (dC3, dT5); (dC2, dT6); (dC4, dT5); (dC3, dT6); (dC2, dT7); (dC5, dT5); (dC4, dT6) and (dC3, dT7) with or without terminal phosphate groups have been isolated on a preparative scale from hydrolysates of depurinated herring sperm DNA by the following procedure. Herring sperm DNA (1 kg) is chemically depurinated and partially hydrolysed to a mixture of pyrimidine nucleotides. The partial hydrolysate is first separated into a low- and a high-molecular-weight pyrimidine nucleotide mixture by column chromatography on DEAE-cellulose. The mixture of high-molecular-weight pyrimidine nucleotides is subsequently fractionated on QAE-Sephadex. Impurities which are not fully removed by column chromatography are separated by paper chromatography. The compositions of the mixtures of sequence isomers are determined from the data of column, paper and homochromatography; from absorption characteristics and by enzymatic degradation.
D000818:Animals; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D004247:DNA / Q000032:analysis*; D005399:Fishes / Q000378:metabolism*; D006868:Hydrolysis; D008297:Male; D009838:Oligodeoxyribonucleotides / Q000302:isolation & purification*; D009841:Oligonucleotides / Q000302:isolation & purification*; D011742:Pyrimidine Nucleotides / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D013094:Spermatozoa / Q000032:analysis*
D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D011742:Pyrimidine Nucleotides; D004247:DNA
Präparative Isolierung von Hexa-, Okta-, Nona- und Decapyrimidinoligonucleotiden aus Hydrolysaten depurinierter Heringsspermen DNA.