Quantitative determination of 3-nitropropionic acid and 3-nitropropanol in plasma by HPLC.
The separation and determination of 3-nitropropanol (NPOH) and 3-nitropropionic acid (NPA) by reverse-phase high-pressure liquid chromatography (HPLC) is described. The system consists of a Micropak MCH-5 column eluted isocratically with 0.15% orthophosphoric acid (pH 2.0). This technique permits the simultaneous determination of NPA and NPOH in bovine and ovine plasma.
D000433:1-Propanol / Q000097:blood; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D009574:Nitro Compounds; D020005:Propanols; D011422:Propionates / Q000097:blood*; D012756:Sheep
D009574:Nitro Compounds; D020005:Propanols; D011422:Propionates; C024522:3-nitro-1-propanol; D000433:1-Propanol; C015392:3-nitropropionic acid