High-performance liquid chromatographic method for the determination of trace amounts of acetaminophen in plasma.
A method is described for the rapid, sensitive, and precise quantitation of acetaminophen in human plasma. The assay involved a single acetonitrile extraction and high-performance liquid chromatographic analysis using a reverse-phase column, with a mobile phase of methanol and water. The limit of quantitation of acetaminophen by this method was 8 ng/mL; only 0.1 mL of the plasma sample was required for the determination. N-Propionyl-p-aminophenol was used as the internal standard.
D000082:Acetaminophen / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007700:Kinetics; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000082:Acetaminophen