Accelerated fluid endocytosis and re-exocytosis by lysosomal storage disease fibroblasts.
Fluid-phase endocytosis was significantly faster in three lines of human lysosomal storage disease fibroblasts (two I cells and one Sandhoff's) than in three normals. Re-exocytosis of fluid showed similar kinetics in all lines when release was expressed as a percentage of the radioactivity associated with the cells at the commencement of the measurement. This indicates a greater absolute exocytosis of radioactive sucrose from the storage disease cells. The data imply that the interactions of lysosomes with other cellular membranes are perturbed in the storage diseases.
D002460:Cell Line; D004705:Endocytosis; D005089:Exocytosis; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D007700:Kinetics; D009081:Mucolipidoses / Q000473:pathology / Q000503:physiopathology*; D012497:Sandhoff Disease / Q000473:pathology / Q000503:physiopathology*; D013395:Sucrose / Q000378:metabolism*
D013395:Sucrose