[Isolation and features of the functioning of mitochondria of the small intestine mucosa in the rat].
A modification is suggested for the method of intact mitochondria isolation from rat thin intestine mucosa. Mitochondria are obtained which can in vitro transport Ca2+ simultaneously with phosphorylation of the added ADP. Kinetic advantages of succinate in providing oxidative phosphorylation as compared with NAD-dependent substrates are much more pronounced in mitochondria of the thin intestine mucosa than in other tissues, and Ca2+ transport occurred only under succinate oxidation. As compared with liver mitochondria these mitochondria are shown to be more sensitive to adrenaline and calcium action.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002458:Cell Fractionation / Q000379:methods; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000648:ultrastructure*; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains; D013386:Succinates / Q000378:metabolism
D013386:Succinates; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D002118:Calcium
Vydelenie i osobennosti funktsionirovaniia mitokhondriĭ slizistoĭ obolochki tonkogo kishechnika krysy.