title: Recovery of megakaryocyte thromboxane production in vitro after aspirin inhibition.
abstract: Megakaryocytes isolated in high purity from guinea pigs produced thromboxane B2 in response to exogenously provided arachidonic acid. This production was inhibited by in vitro treatment with acetylsalicylic acid with a concentration response relationship similar to that seen in platelets. During in vitro culture, the aspirin-treated megakaryocytes recovered thromboxane synthetic ability. Following a lag of 12 hours, recovery of megakaryocyte thromboxane production resumed at a rate of 16% of control per day. This recovery was inhibited by the addition of cycloheximide to the culture medium.
mesh_terms: D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D001792:Blood Platelets / Q000378:metabolism; D002478:Cells, Cultured; D006168:Guinea Pigs; D007700:Kinetics; D008533:Megakaryocytes / Q000187:drug effects / Q000378:metabolism*; D011863:Radioimmunoassay; D013929:Thromboxane B2 / Q000096:biosynthesis*; D013931:Thromboxanes / Q000096:biosynthesis*
chemical_list: D013931:Thromboxanes; D013929:Thromboxane B2; D001241:Aspirin