Differential effects of androgens and glucocorticoids on regulation of androgen receptor concentrations and cell growth.
This report describes androgen induced up-regulation of intracellular androgen receptor concentrations in the DDT1MF-2 cell line derived from the hamster ductus deferens and the R3327H-G8-A1 line derived from the Dunning prostate adenocarcinoma. Within 6 h of exposure to androgens the receptor concentration is increased approximately 2-fold. Incorporation of dense amino-acids and subsequent analysis by sucrose density gradient centrifugation indicates that the observed increase in receptors is possibly due to de novo androgen receptor synthesis. In both cell lines this increase is inhibited by 30-50% within 6 h and completely during a subsequent 18 h period by the potent glucocorticoid triamcinolone acetonide (TA). TA also inhibits the growth of both cell lines and antagonizes the stimulatory effect of androgens. Flow cell cytometry studies indicate that TA blocks the cells in the G1 phase of the cell cycle. This event may be associated with regulation of androgen receptor concentrations.
D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D013196:Dihydrotestosterone / Q000378:metabolism; D005434:Flow Cytometry; D005834:Genital Neoplasms, Male / Q000378:metabolism; D005938:Glucocorticoids / Q000494:pharmacology*; D008297:Male; D011944:Receptors, Androgen / Q000187:drug effects / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D014222:Triamcinolone Acetonide / Q000494:pharmacology; D014649:Vas Deferens
D000728:Androgens; D005938:Glucocorticoids; D011944:Receptors, Androgen; D011987:Receptors, Steroid; D013196:Dihydrotestosterone; D014222:Triamcinolone Acetonide