Urinary N-acetyl-beta-D-glucosaminidase (NAG) isoenzyme profiles: a tool for evaluating nephrotoxicity of aminoglycosides and cephalosporins.
The study of urinary NAG isoenzymes in different nephrotoxic states shows that each antibiotic is characterised by a specific isoenzyme profile which probably reflects the very nature of its toxic mechanism. Those aminoglycosides which are responsible for important elimination of B- and I-forms would directly induce the synthesis of these isoenzymes in the endoplasmic reticulum. Cephalosporins, which are less nephrotoxic, present profiles which are closer to those obtained for normal urine. A combination aminoglycoside plus cephalosporin, though globally more nephrotoxic than aminoglycosides alone, is characterised by relatively poorer profiles of B- and I-forms. This could be linked to the reciprocal interaction of one antibiotic with the toxic mechanisms of the other.
D000118:Acetylglucosaminidase / Q000302:isolation & purification / Q000652:urine*; D000617:Aminoglycosides / Q000009:adverse effects*; D002511:Cephalosporins / Q000009:adverse effects*; D002848:Chromatography, DEAE-Cellulose; D004359:Drug Therapy, Combination; D006596:Hexosaminidases / Q000652:urine*; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification / Q000652:urine*; D007674:Kidney Diseases / Q000139:chemically induced* / Q000175:diagnosis
D000617:Aminoglycosides; D002511:Cephalosporins; D007527:Isoenzymes; D006596:Hexosaminidases; D000118:Acetylglucosaminidase