Electron-capture gas chromatographic assays of 5-nitroimidazole class of antimicrobials in blood.
Sensitive and selective electron-capture gas chromatographic methods for the determination of N-1-substituted 5-nitroimidazole class of antiprotozoals from blood are described. Metronidazole, secnidazole and ornidazole having a hydroxyl function in the N-1 substitution, were converted to their respective trimethylsilyl derivatives before chromatography on an OV-11 column. Tinidazole and satranidazole, devoid of the hydroxy group but containing a sulphur atom in the molecule, were chromatographed as such on the same stationary phase. Blood levels as low as 50 ng/ml for all the 5-nitroimidazoles have been measured with good precision. The methods can be readily utilized for pharmacokinetic studies.
D000994:Antitrichomonal Agents / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D008795:Metronidazole / Q000031:analogs & derivatives / Q000097:blood; D009593:Nitroimidazoles / Q000097:blood*; D009950:Ornidazole / Q000097:blood; D014011:Tinidazole / Q000097:blood
D000994:Antitrichomonal Agents; D009593:Nitroimidazoles; D014011:Tinidazole; D008795:Metronidazole; C034473:satranidazole; D009950:Ornidazole; C016724:secnidazole