Irreversible binding of conjugated bilirubin to albumin in cholestatic rats.
A diazo-positive fraction of serum bilirubin that is irreversibly bound to albumin has been shown to accumulate in serum of patients with cholestasis. In the present study, a cholestatic animal model was used to determine the chemical nature of the bilirubin species involved in its formation. The data indicate that conjugated bilirubin is the precursor of "albumin-bound bilirubin" and that the presence or absence of light does not affect its formation. An albumin-bound bilirubin-complex indistinguishable from the complex detected in cholestatic sera from patients or in bile duct-ligated Sprague-Dawley rats can be formed in vitro in sera enriched in conjugated bilirubin at 37 degrees C, pH 7.4.
D000818:Animals; D001652:Bile Ducts / Q000502:physiology; D001663:Bilirubin / Q000097:blood*; D002779:Cholestasis / Q000378:metabolism*; D006801:Humans; D008026:Ligation; D008027:Light; D008297:Male; D008954:Models, Biological; D011485:Protein Binding / Q000528:radiation effects; D051381:Rats; D011911:Rats, Gunn; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000378:metabolism*
D012709:Serum Albumin; D001663:Bilirubin