Does the concentration of DNA (Co) and the time of incubation (t) as parameters of Cot influence the thermal stability of the DNA duplexes?
It has been shown in a previous paper (8) that the prime product of reassociation of related DNA sequences under open experimental conditions are mismatched duplexes which undergo 'maturation' upon further incubation. Due to this feature, the Tm value of the duplexes of a large number of DNAs is strongly dependent on the Cot value. Here we present data showing that the Tm of the duplexes of such type of DNAs depends also on the concentration of DNA in the range of one and the same Cot value. The significance of this finding in studying the taxonomic relationship by DNA-DNA hybridisation is discussed.
D004269:DNA, Bacterial; D004305:Dose-Response Relationship, Drug; D006358:Hot Temperature; D006860:Hydrogen Bonding; D007700:Kinetics; D009695:Nucleic Acid Renaturation
D004269:DNA, Bacterial