Isolation and convulsant effect of the 'insulin-like' proteins from the pancreas of the Brazilian opossum Didelphis albiventris.
An opossum pancreatic insulin-like protein fraction was obtained at pH 5.2 which has high electrophoretic mobility. At pH 8.2, another pancreatic insulin-like protein fraction was obtained with low electrophoretic mobility. Isoelectric focusing studies showed that the high molecular weight protein has a pI value 8.35 and the low molecular weight protein has pI value of 3.9 when used at pH range between 3.5-10.0. The purified high and low molecular weight positive aldehyde-fuchsin proteins induced convulsions when administered subcutaneously to mice.
D000818:Animals; D003292:Convulsants / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008297:Male; D008970:Molecular Weight; D009893:Opossums / Q000378:metabolism*; D010179:Pancreas / Q000032:analysis*; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*; D013045:Species Specificity; D014020:Tissue Extracts / Q000032:analysis
D003292:Convulsants; D011506:Proteins; D014020:Tissue Extracts