Quantitative determination of chlorpromazine and thioridazine by high-performance thin layer chromatography.
An assay for quantitation of chlorpromazine and thioridazine in patient plasma is developed. The procedure utilizes high-performance thin layer chromatography for separation and in situ absorption densitometry for quantitation. Depending upon the HPTLC plate size, 30 to 60 samples may be processed in less than 6 hr, with an intra-assay coefficient of variation of less than 4%. The procedure can be used to measure as little as 10 ng/ml of drug in plasma, well below expected concentrations in patients receiving these medications. Investigations of extraction procedures, sample application procedures, chromatographic conditions, sample derivatization, and in situ absorption densitometry are described.
D002746:Chlorpromazine / Q000097:blood*; D002855:Chromatography, Thin Layer / Q000379:methods; D006801:Humans; D013881:Thioridazine / Q000097:blood*
D013881:Thioridazine; D002746:Chlorpromazine