Immunoregulation in experimental schistosomiasis. II. Soluble egg antigen-induced chronic spleen cell augmentation of baseline lymphocyte reactivity.
Spleen cells from mice harboring infections of Schistosoma mansoni for 20 weeks exposed to Con A or to soluble schistosome egg antigenic preparation (SEA), treated with Mitomycin C (Mc), and cocultured with spleen cells from either normal or infected mice caused an augmented baseline [3H]TdR incorporation by the otherwise unstimulated responder cells. This regulation required an in vitro induction phase. SEA-exposed, Mc-treated normal spleen cells had no effect on responder cell cultures. SEA-stimulated, Mc-treated chronic spleen cell augmentation was effective on responder cell populations from either normal mice or mice infected with S. mansoni for 8 weeks. Augmentation was most pronounced when assayed on cells from infected mice assayed over a 5-day incubation. In addition, it is demonstrated that these Con A- and SEA-elicited activities are mediated by soluble mediators which lack H-2 restriction.
D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D003208:Concanavalin A; D004261:DNA Replication; D004195:Disease Models, Animal; D005260:Female; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D010063:Ovum / Q000276:immunology; D012550:Schistosoma mansoni / Q000276:immunology; D012552:Schistosomiasis / Q000276:immunology*; D013154:Spleen / Q000276:immunology
D000941:Antigens; D003208:Concanavalin A