title: A method for screening diuretic agents in the rat.
abstract: A reproducible screening method for diuretic agents has been developed based upon the selection of a group of homogenous rats with a similar excretory pattern of water and electrolytes. Normal saline (4% body weight) was used as a hydrating fluid to select appropriate rats and to validate the diuretic activity of standard diuretics. The method required only a small number of rats for screening compounds and produced consistent responses in increasing water and electrolyte excretion.
mesh_terms: D000818:Animals; D004231:Diuresis / Q000187:drug effects; D004232:Diuretics / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004232:Diuretics