Pancreatic duct glands. II. Lectin binding affinities of ductular epithelium, ductular glands, and Brunner glands.
Secretory epithelia and ductular glands were studied in main pancreatic ducts of guinea pig, rabbit, rat, and man. Brunner glands were studied for comparison. Semithin sections from Epon-embedded tissues were etched with sodium methylate and incubated with horseradish peroxidase-conjugated lectins. Columnar cells in the epithelium of pancreatic ducts are endowed with well-developed microvillar borders. These apical regions strongly stain with Lotus A-, wheat germ-, and Ricinus I-lectins. Basolateral plasma membranes bind Ricinus I-, Ulex europaeus I-, and wheat germ-lectins. Cytosomes in the supranuclear regions of epithelial cells are interpreted as secretory granules. These droplets are marked by wheat germ-lectin and to a lesser degree by Ricinus I- and Ulex europaeus I-lectins. Ductular glands of the main pancreatic ducts contain secretions that bind Helix-, wheat germ-, and Ulex europaeus I-lectins. Their apical and basolateral cell membranes deeply stain with wheat germ- and Ulex europaeus I-lectins. Secretions of Brunner glands bind Ricinus I-, Ulex europaeus I-, Helix-, and wheat germ lectins. Their apical and basolateral cell membranes stain with Ricinus I- and wheat germ-lectins. - Species differences in lectin-binding affinities of complex carbohydrates were observed and are described.
D000818:Animals; D002011:Brunner Glands / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis; D004386:Duodenum / Q000032:analysis*; D004848:Epithelium / Q000032:analysis; D005088:Exocrine Glands / Q000032:analysis*; D006168:Guinea Pigs; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins; D010183:Pancreatic Ducts / Q000032:analysis* / Q000648:ultrastructure; D010503:Periodic Acid-Schiff Reaction; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D002241:Carbohydrates; D037102:Lectins