Characteristics of a human epidermal squamous carcinoma cell line at different extracellular calcium concentrations.
A continuous line derived from a human skin squamous cell carcinoma has been grown in media of high, normal and low Ca2+ concentrations. The growth rate was unaffected by the Ca2+ levels even though morphological changes were observed. Desmosomes were absent at low Ca2+ and areas of cell piling were observed at high Ca2+. Cell protein staining patterns on polyacrylamide gels were identical for cells grown at the three Ca2+ levels. The variations were minor for the glycoproteins reacted with 125I-conA. Lactoperoxidase iodination revealed changes in cell surface proteins, most markedly in the emergence of new proteins at high Ca2+.
D002118:Calcium / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000032:analysis / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D003896:Desmosomes / Q000187:drug effects; D004817:Epidermis / Q000473:pathology; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis; D009363:Neoplasm Proteins / Q000032:analysis*; D012878:Skin Neoplasms / Q000032:analysis / Q000473:pathology*
D003470:Culture Media; D006023:Glycoproteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D002118:Calcium