Identification of spectrin and protein 4.1-like proteins in mammalian lens.
Human, bovine, canine, and rabbit lenses were found to contain proteins which cross-react with anti-4.1 serum and which have molecular weights similar to erythrocyte proteins 4.1a and 4.1b (approximately 80 kd). Additionally, bovine, canine, and rabbit lenses contain a 4.1-like protein of approximately 125 kd which is absent from human lens. Proteins which cross-react with antibody to human erythrocyte spectrin were also detected. The human lens showed weak cross-reaction of bands of 240 kd and 225 kd, and a more intense cross-reaction of a band of 235 kd. Canine and bovine lenses showed weak cross-reaction with only the bands at 240 kd and 235 kd. The lens 240 kd band of all species also demonstrated calcium-dependent binding of calmodulin. Our results indicate that proteins related to, but distinct from, erythrocyte protein 4.1 and spectrin are found in mammalian lens.
D000818:Animals; D000936:Antigen-Antibody Complex; D001798:Blood Proteins / Q000302:isolation & purification*; D002417:Cattle; D003429:Cross Reactions; D003598:Cytoskeletal Proteins; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000032:analysis; D006801:Humans; D007106:Immune Sera; D007908:Lens, Crystalline / Q000032:analysis*; D008565:Membrane Proteins; D008970:Molecular Weight; D009479:Neuropeptides; D011817:Rabbits; D013045:Species Specificity; D013049:Spectrin / Q000302:isolation & purification*
D000936:Antigen-Antibody Complex; D001798:Blood Proteins; D003598:Cytoskeletal Proteins; D007106:Immune Sera; D008565:Membrane Proteins; D009479:Neuropeptides; C028841:erythrocyte membrane band 4.1 protein; C120557:erythrocyte membrane protein band 4.1-like 1; D013049:Spectrin