A convenient source of CFU-s inhibitors: the fetal calf liver.
The presence of a bone-marrow stem-cell inhibitor able to prevent CFU-s entry into DNA synthesis after cytosine arabinoside (Ara-C) treatment has been detected in 7-month-old fetal calf liver. The inhibitory fraction was obtained through ultrafiltration of a delipidated tissue extract powder and purified by BioGel-P-2 chromatography. The elution pattern on Sephadex G10 is similar to that of the bone-marrow inhibitory extract previously obtained from fetal calf bone marrow. It corresponds to a low-molecular-weight molecule (MW less than 2000), devoid of species specificity and having no inhibitory effect on GM-CFC proliferation.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002850:Chromatography, Gel; D003561:Cytarabine / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D005260:Female; D005333:Fetus / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000032:analysis / Q000166:cytology / Q000187:drug effects*; D007399:Interphase; D008099:Liver / Q000032:analysis / Q000378:metabolism*; D008297:Male; D051379:Mice; D013347:Subcellular Fractions / Q000032:analysis; D014020:Tissue Extracts / Q000032:analysis*
D014020:Tissue Extracts; D003561:Cytarabine; D004247:DNA