Vancomycin: a rapid HPLC assay for a potent antibiotic.
Vancomycin 'Vancocin HCl' is a potent antimicrobial used most frequently in the treatment of gram positive septicemias. Ototoxicity has occurred at serum levels of 80 micrograms/mL. A rapid, sensitive assay for vancomycin that utilizes high-pressure liquid chromatography with ultraviolet detection, and requires only 100 microL of serum, is presented. The method shows excellent linearity and precision throughout the therapeutic and toxic range.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006311:Hearing Disorders / Q000139:chemically induced; D006801:Humans; D014640:Vancomycin / Q000009:adverse effects / Q000097:blood*
D014640:Vancomycin