title: Inhibition of pinocytosis and induction of release of lysosomal contents by lysosomal overload of arterial smooth muscle cells in vitro.
abstract: Lysosomal overload was induced experimentally in cultured aortic smooth muscle cells by incubation with chloroquine or sucrose. Lysosomal overload was accompanied by a marked reduction in pinocytosis and induced the release of lysosomal contents into the medium. Thus, previously accumulated pinocytic tracer and beta-glucuronidase, a lysosomal enzyme, were released into the medium whereas lactate dehydrogenase, a cytosolic enzyme, was not.
mesh_terms: D000818:Animals; D001158:Arteries / Q000378:metabolism; D002478:Cells, Cultured; D005966:Glucuronidase / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D010873:Pinocytosis; D013552:Swine
chemical_list: D005966:Glucuronidase