Recombinant-derived chicken growth hormone used for radioimmunoassay.
The use of recombinant-derived chicken growth hormone (rcGH) in an avian growth hormone (GH) radioimmunoassay (RIA) procedure is described. Antiserum to turkey GH bound 125I-labeled rcGH, and unlabeled rcGH or turkey GH displaced binding in a dose-related manner. The dose-response curves of sera and pituitary extract from chickens and turkeys were parallel to the rcGH standard curve. Sera from hypophysectomized (hypox) chickens and turkeys produced no dose-response and did not inhibit binding of labeled rcGH. Recovery of rcGH added to hypox sera was quantitative. Modification of the homologous turkey GH RIA protocol of Proudman and Wentworth (1) to use rcGH made possible either an increase in assay sensitivity or a 3-day reduction in incubation time.
D000818:Animals; D002645:Chickens; D004274:DNA, Recombinant; D013006:Growth Hormone / Q000032:analysis* / Q000097:blood / Q000235:genetics; D007016:Hypophysectomy; D010902:Pituitary Gland / Q000032:analysis; D011863:Radioimmunoassay / Q000379:methods; D014422:Turkeys
D004274:DNA, Recombinant; D013006:Growth Hormone