Plasma levels of free and total catecholamines and two deaminated metabolites in man--rapid deconjugation by heat in acid.
We have described a procedure for deconjugation of plasma catecholamines, norepinephrine (NE), epinephrine (E) and dopamine (DA) and two catecholamine metabolites 3,4-dihydroxymandelic acid (DOMA) and 3,4-dihydroxyphenylglycol (DOPEG). Heat at 100 degrees C of the acidified specimen, pH 0.8, produced complete deconjugation of catecholamines in 7 minutes and of metabolites in 5-7 minutes. Subsequently all five products were simultaneously measured with a radioenzymatic assay. However, hydrolysis for 7 minutes produced approximately a loss of 5% in DA and E, 15% in NE and 50% in the metabolites. The percent of free compound in the plasma of 11 normotensive and healthy subjects was 23 +/- 16 for NE, 20 +/- 8 E, 0.8 +/- 1 DA, 20 +/- 7 DOMA and 42 +/- 12 for DOPEG. Similar results were obtained in a random specimen of six patients with primary hypertension. In a group of four patients with pheochromocytoma free levels of NE, DOPEG and DOMA were significantly greater than in the other two groups, whereas conjugates were not. The intravenous administration of NE or the activation of sympathetic nervous system by standing combined with exercise for 15 minutes did not produce a change in the levels of plasma conjugates. These findings suggest that short changes in plasma catecholamines are better reflected in the free than the conjugated part.
D002395:Catecholamines / Q000097:blood*; D004355:Drug Stability; D006018:Glycols / Q000097:blood*; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008333:Mandelic Acids / Q000097:blood*; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000097:blood*; D005082:Physical Exertion
D002395:Catecholamines; D006018:Glycols; D008333:Mandelic Acids; D008734:Methoxyhydroxyphenylglycol; C000989:3,4-dihydroxymandelic acid; C010117:3,4-dihydroxyphenylglycol