Species identification of blood and saliva stains by enzyme-linked immunoassay (ELISA) using monoclonal antibody.
An indirect enzyme-linked immunoassay (ELISA) method for the identification of human blood and saliva stains is reported. The method uses a monoclonal antibody which reacts with human immunoglobulin G (IgG) in extracts of blood and saliva stains up to 16 months old. Semen stain extracts gave weak or negative results. For routine screening purposes dilutions of 1:1000 for bloodstain extracts and 1:100 for saliva stain extracts would be suitable. Of 32 other animal species tested, only chimpanzee, mouse, rat, and eel cross-reacted significantly, and the presence of the last three was clearly indicated by appropriate controls. The monoclonal antibody gave poor results in the crossover and gel diffusion techniques.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001801:Blood Stains; D004524:Eels; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D002679:Pan troglodytes; D051381:Rats; D012463:Saliva / Q000032:analysis*; D012661:Semen / Q000032:analysis; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G