Cell-free translation of human uroporphyrinogen decarboxylase mRNAs.
Uroporphyrinogen decarboxylase was synthesized in a reticulocyte lysate cell-free system under the direction of messenger RNAs isolated from human fetal liver and from human reticulocytes. The enzyme was specifically isolated by immuno affinity chromatography. Analysis of the translation products showed that uroporphyrinogen decarboxylase was synthesized in vitro with its mature molecular weight. This enzyme represented 0.04% of the total neosynthesized proteins under the direction of fetal liver mRNA and about ten times less (0.005%) with reticulocyte mRNA.
D000755:Anemia, Sickle Cell / Q000097:blood; D002262:Carboxy-Lyases / Q000235:genetics*; D002474:Cell-Free System; D005260:Female; D005333:Fetus; D006801:Humans; D008099:Liver / Q000201:enzymology; D008970:Molecular Weight; D011247:Pregnancy; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012156:Reticulocytes / Q000378:metabolism; D014575:Uroporphyrinogen Decarboxylase / Q000235:genetics* / Q000302:isolation & purification
D012333:RNA, Messenger; D002262:Carboxy-Lyases; D014575:Uroporphyrinogen Decarboxylase