Determination of a new antihypertensive agent (2R,4R)-2-(2-hydroxyphenyl)-3-(3-mercaptopropionyl)-4- thiazolidinecarboxylic acid in blood by high-performance liquid chromatography with electrochemical detection.
A sensitive method has been developed for the determination of a new antihypertensive agent, (2R,4R)-2-(2-hydroxyphenyl)-3-(3-mercaptopropionyl)-4-thiazolidinecar boxylic acid (I), in human blood. Because the drug is unstable in biological fluids, it was immediately derivatized by treating the freshly drawn blood specimens with N-(4-anilinophenyl)maleimide. The adduct was separated and determined by high-performance liquid chromatography with electrochemical detection on a reverse-phase column. The assay method was satisfactory with respect to the sensitivity and precision, providing a quantitation limit of 2 ng/ml and coefficient of variation of 3%. Preliminary pharmacokinetic data were obtained by orally administering I to two patients with essential hypertension.
D015097:3-Mercaptopropionic Acid / Q000031:analogs & derivatives / Q000097:blood*; D000959:Antihypertensive Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D005260:Female; D006801:Humans; D007700:Kinetics; D013438:Sulfhydryl Compounds; D053778:Thiazolidines
D000959:Antihypertensive Agents; D013438:Sulfhydryl Compounds; D053778:Thiazolidines; D015097:3-Mercaptopropionic Acid; C033437:rentiapril