Identification of 6-acetyl-7-methyl-7,8-dihydropterin as a degradation product of 5,10-methenyl-5,6,7,8-tetrahydromethanopterin.
During purification procedures and upon aerobic heating with alkali a green-yellow degradation fluorescent product (GY) was formed from 5,10-methenyl-5,6,7,8-tetrahydromethanopterin, an intermediate in the reduction of CO2 to methane [J. T. Keltjens, L. Daniels, H. G. Janssen, and G. D. Vogels (1983) Eur. J. Biochem. 130, 545-552]. GY was suggested to be a 6-(1-oxo)-7,8-dihydropterin. On the basis of the spectral properties and the results of degradation studies, it was now shown that the structure of GY is 6-acetyl-7-methyl-7,8-dihydropterin. This structure was confirmed by synthesis of the compound and other reference substances.
D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D019605:Euryarchaeota / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D011622:Pterins / Q000302:isolation & purification* / Q000378:metabolism*; D013053:Spectrophotometry
C040753:5,10-methenyl-5,6,7,8-tetrahydromethanopterin; C040752:6-acetyl-7-methyl-7,8-dihydropterin; D011622:Pterins