Hyperthermia enhances the reactivation of irradiated adenovirus in HeLa cells.
The reactivation of U.V.-irradiated adenovirus 2 in HeLa cells is enhanced 8-9 fold if the cells are given a brief hyperthermic shock before infection. Maximum reactivation is achieved by heating for 10 min at 45.5 degrees C and with a delay of 36 h between heating and infection. The induction process requires protein synthesis only during the 3 h period immediately following heating; cycloheximide does not prevent the expression of enhanced reactivation if added to the cells after this time. Heat-enhanced reactivation exhibits properties similar in some respects to radiation-enhanced reactivation and indicates an increased capacity of the heated cells to tolerate DNA damage.
D000256:Adenoviridae / Q000254:growth & development* / Q000528:radiation effects; D003513:Cycloheximide / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D006367:HeLa Cells; D006358:Hot Temperature; D006801:Humans; D013997:Time Factors; D014466:Ultraviolet Rays; D014775:Virus Activation / Q000187:drug effects
D003513:Cycloheximide