In vitro rates of oxidation and gluconeogenesis from L(+)- and D(-)lactate in bovine tissues.
Slices of bovine kidney cortex, liver, heart and sternomandibularis muscle actively metabolized D- and L-lactate. Rates of D-lactate oxidation were greatest in kidney cortex followed by heart and liver with muscle exhibiting the lowest rates. L-lactate oxidation was greatest in kidney cortex followed by heart with liver and muscle exhibiting similar rates. Rates of oxidation of gluconeogenesis were similar for D- and L-lactate at 0.1 mm lactate but D utilization, as a percent of L, decreased as substrate concentrations increased to 50 mM. Bovine tissues appear to possess significant potential for D(-)lactate utilization. Estimates of this and possible interactions are discussed.
D000818:Animals; D002417:Cattle / Q000378:metabolism*; D005943:Gluconeogenesis; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000378:metabolism; D007773:Lactates / Q000378:metabolism*; D019344:Lactic Acid; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D010084:Oxidation-Reduction; D013237:Stereoisomerism
D007773:Lactates; D019344:Lactic Acid