title: Invaginated apical vacuoles in the cells of the proximal convoluted tubule in the rat kidney.
abstract: Following perfusion fixation of the rat kidney with glutaraldehyde the proximal tubule cells display small apical vacuoles, large apical vacuoles, and apical vacuoles in which a part of the limiting membrane is invaginated into the vacuole. These invaginated apical vacuoles occur more frequently in proximal convoluted tubules than in proximal straight tubules. One tubular cell may contain apical vacuoles of different sizes and stages of invagination, ranging from larger vacuoles with a wide lumen and a small area of invaginated membrane to smaller elements with no apparent lumen and a large area of invaginated membrane. Invaginated apical vacuoles lie either singly in the cytoplasm or close to the membranes of other apical vacuoles, but never in contact with the cell membrane or the membranes of lysosomes, endoplasmic reticulum, Golgi apparatus, mitochondria and peroxisomes. These findings suggest that the invaginated apical vacuoles are not fixation artifacts, but rather develop in living state in cells of the proximal tubule from spherical endocytotic elements.
mesh_terms: D000375:Aging; D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D007668:Kidney / Q000254:growth & development; D007687:Kidney Tubules, Proximal / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009940:Organoids / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains; D014617:Vacuoles / Q000648:ultrastructure*