A simplified procedure for separating amniotic fluid phospholipids with thin-layer chromatography.
A simple, unidimensional, thin-layer-chromatographic separation of amniotic fluid phospholipids involving detection with a phosphorus-specific spray was compared to the two-dimensional, thin-layer-chromatographic method described by Gluck et al. There were close correlations between the two techniques for lecithin/sphingomyelin ratios and for the presence of phosphatidylglycerol. The advantages of the unidimensional method are a shorter procedure time, the ability to analyze multiple samples on a single plate and the simplicity of a single-solvent system.
D000649:Amniocentesis; D000653:Amniotic Fluid / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000302:isolation & purification*; D011247:Pregnancy; D013109:Sphingomyelins / Q000032:analysis
D010713:Phosphatidylcholines; D010743:Phospholipids; D013109:Sphingomyelins