[Determination of carbohydrates in foods in the ppm range using high performance liquid chromatography with UV detection. II].
After derivatization (benzoylation) it is possible to determine carbohydrates (mono- to tetrasaccharides) in foods by normal phase as well by reversed phase-HPLC. The method shows high selectivity and high sensitivity. The detection limit for sucrose is 0.2 ppm respectively 2 ng (normal phase) and 0.3 ppm respectively 3 ng (reversed phase). For clean up a maximum of 8 min and an additional 70 min rest period are necessary.
D002851:Chromatography, High Pressure Liquid; D004040:Dietary Carbohydrates / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*
D004040:Dietary Carbohydrates
Hochleistungs-flüssigchromatographische Bestimmung von Kohlenhydraten mit UV-Detektion im ppm-Bereich in Lebensmitteln II.