Preparation and characterization of monoclonal antibodies against phenobarbital-inducible cytochrome P-450.
Monoclonal antibodies against cytochrome P-450 were prepared from phenobarbital-induced rat liver microsomes. The immunoglobulin classes and subclasses, as well as the binding capacity to cytochrome P-450, of the different antibodies were characterized. Their specificity was verified by various techniques and seemed to correspond to a single form of cytochrome P-450, the major phenobarbital-inducible form. However, the antibodies were unable to inhibit completely the monooxygenase activities investigated. These antibodies may constitute very specific and powerful analytical tools for characterizing and quantifying cytochrome P-450 isoenzymes.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004790:Enzyme Induction; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000096:biosynthesis; D010634:Phenobarbital / Q000494:pharmacology*; D011817:Rabbits
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D010634:Phenobarbital