Interrelationships among distinct idiotypic specificities.
The fine idiotypic properties of a hybridoma (H51.85.2) anti-GAT antibody which lacks both CGAT and GA-1 idiotypes are described. We identified another idiotype, termed GA-2 idiotype, on H51.85.2 hybridoma antibody. The GA-2 idiotype is present in all mouse strains tested and is induced by GA-related antigenic determinants. An interesting relationship between GA-1 and GA-2 idiotype on hybridoma anti-GA antibodies was observed. All GA-1+ hybridomas also express GA-2 idiotype. In contrast, GA-2+ hybridomas can express a full set, a fraction, or none of the GA-1 idiotypic determinants. The data together with previous amino acid sequence studies strongly suggest that H51.85.2 hybridoma antibody was derived from the same VH and VL genes encoding the GA-1+ hybridoma antibodies.
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D006825:Hybridomas / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000032:analysis / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D010455:Peptides / Q000276:immunology; D011108:Polymers
D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D010455:Peptides; D011108:Polymers; C020749:GAT