title: NADH-dependent tiglyl-CoA reduction in disrupted mitochondria of Ascaris suum.
abstract: The incubation of 2-methylcrotonyl-CoA with either succinate or NADH and disrupted Ascaris suum mitochondria results in substantial 2-methylbutyrate formation. Both membrane-bound and soluble components are required and the NADH-dependent reduction is rotenone sensitive, suggesting the involvement of the electron-transport chain. Rat liver mitochondria, incubated under similar conditions, did not catalyze 2-methylbutyrate formation. However, the substitution of A. suum mitochondrial membranes for rat liver membranes stimulated 2-methylbutyrate formation, emphasizing the differences in electron-transport in these two organelles.
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism*; D000693:Anaerobiosis; D000818:Animals; D001200:Ascaris / Q000378:metabolism*; D004734:Energy Metabolism; D008928:Mitochondria / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010766:Phosphorylation; D051381:Rats
chemical_list: D000214:Acyl Coenzyme A; D009243:NAD; C033618:tiglyl-coenzyme A