Radioimmunoassay of a glucose-containing tetrasaccharide using a monoclonal antibody.
Monoclonal antibodies were produced in BALB/cJ mice against a urinary glucose-containing tetrasaccharide (Glc)4 coupled to keyhole limpet hemocyanin (KLH). The immune response was studied using 7 different strains of mice and 11 different immunization protocols. From 6 fusions using the Sp2/0 myeloma cell line 2 hybrids were obtained producing antibodies (subtype IgG2b and IgG3, respectively) that were capable of binding reduced tetrasaccharide. Specificity studies showed that one of these antibodies (61.1) was useful for radioimmunoassay of (Glc)4.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D000941:Antigens / Q000008:administration & dosage; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D005260:Female; D006433:Hemocyanins; D006825:Hybridomas / Q000276:immunology; D007114:Immunization / Q000379:methods; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009844:Oligosaccharides / Q000008:administration & dosage / Q000032:analysis* / Q000276:immunology; D011863:Radioimmunoassay; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000941:Antigens; D009844:Oligosaccharides; C033620:glucose tetrasaccharide; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin