Effects of partial hepatectomy on RNA polymerase activities in mouse liver.
Chromatin-bound and poly[d(A-T)]dependent RNA polymerase I plus III and II activities of mouse liver were analysed 24 and 48 hr after partial hepatectomy. Chromatin-bound RNA polymerase I plus III activity showed an increase of 57% at 24 hr and 51% at 48 hr after partial hepatectomy. There was a decrease in chromatin-bound RNA polymerase II activity of 15% at 24 hr and 34% at 48 hr after partial hepatectomy. There was no significant changes in poly[d(A-T)]dependent RNA polymerase activities. Heparin caused an approximately 10-fold increase in chromatin-bound RNA polymerase II activity. The stimulation by heparin was significantly increased 48 h after partial hepatectomy. Anaesthesia and/or surgery had great influence on RNA polymerase activities. At 24 hr after operation, chromatin-bound RNA polymerase I plus III and II activities were depressed, and the liver cell chromatin was more susceptible to stimulation by heparin.
D000818:Animals; D002843:Chromatin / Q000201:enzymology; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D006493:Heparin / Q000494:pharmacology; D006498:Hepatectomy; D008099:Liver / Q000201:enzymology*; D008115:Liver Regeneration; D008297:Male; D051379:Mice; D011067:Poly dA-dT / Q000494:pharmacology; D012318:RNA Polymerase I / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism; D012320:RNA Polymerase III / Q000378:metabolism
D002843:Chromatin; D011067:Poly dA-dT; D006493:Heparin; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I; D012320:RNA Polymerase III