A new sialyloligosaccharide from human milk: isolation and characterization using anti-oligosaccharide antibodies.
A previously undescribed sialyloligosaccharide has been isolated from human milk using a specific anti-sialyloligosaccharide antibody. Structural studies of the radiolabeled oligosaccharide by enzyme degradation and binding by specific anti-oligosaccharide sera are consistent with the following structure: (sequence in text) The oligosaccharide is present only in milk from donors who secrete A, B, or H blood group substances; this is consistent with the requirement of at least one copy of the Se (Secretor) gene necessary for the synthesis of oligosaccharides with Fuc alpha 1-2Gal . . . linkages.
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005260:Female; D006801:Humans; D008895:Milk, Human / Q000032:analysis*; D009844:Oligosaccharides / Q000302:isolation & purification*; D011247:Pregnancy; D013329:Structure-Activity Relationship; D014316:Tritium
D009844:Oligosaccharides; C016735:sialooligosaccharides; D014316:Tritium