title: Accurate and efficient method for quantification of urinary histamine by gas chromatography negative ion chemical ionization mass spectrometry.
abstract: Because of the recognized inaccuracy and unreliability of currently available methods for the quantification of histamine in biological fluids, a method for quantification of urinary histamine by stable isotope dilution assay with negative ion chemical ionization mass spectrometry has been developed. Following the addition of [2H4]histamine to 1 ml of urine, histamine is extracted into butanol, back-extracted into HCl, derivatized to the pentafluorobenzyl derivative (CH2C6F5)3-histamine, extracted into methylene chloride, and then quantified with negative ion chemical ionization mass spectrometry by selected ion monitoring of the ratio of ions m/z430/434. Twenty samples can be assayed in 2 days. Precision of the assay is +/- 2.7% and the accuracy is 97.6%. Lower limits of sensitivity are approximately 100-500 fg injected on-column. This assay provides a very sensitive, accurate, and efficient method for the quantification of histamine in human urine.
mesh_terms: D005456:Fluorescent Dyes / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006632:Histamine / Q000031:analogs & derivatives / Q000652:urine*; D006801:Humans; D005466:Fluorocarbons
chemical_list: D005456:Fluorescent Dyes; D006632:Histamine; C040915:N,N',N'-tri(pentafluorobenzyl)histamine; D005466:Fluorocarbons