Toxic properties of the Mycoplasma arthritidis ISR-1 membrane.
Isolated membranes of Mycoplasma arthritidis ISR-1 possess toxic properties for rats, mice and chicken embryos. Reactions are dose-dependent. By intravenous injection of 20 mg of membrane protein, a lethal toxicity syndrome resembling shock is induced in Sprague-Dawley rats of 100 g body weight. Mice of approximately 20-g body weight show the same syndrome by intravenous injection of 15 mg of membrane protein. The lethal toxicity for chicken embryos increases from 10 to 80% after injection of 0.3 to 1.5 mg of membrane protein. The LD50 in chicken embryos was found to be 0.5 mg membrane protein. After administration of 10-15 mg of membrane protein, rats showed an increase in temperature from 38 to 41 degrees C within 5 h. Because of the difficulty in measuring the temperature in mice, loss of body weight was taken as the criterion for toxicity. Body weight was reduced up to 16% within 1 day after intravenous injection of 500 micrograms of membrane protein per g of body weight. For sheep red blood cells, M. arthritidis ISR-1 membranes were haemolytic in concentrations of 4 mg of membrane protein and higher. Intradermal injection of 1 mg of membrane protein into guinea-pigs resulted in red swelling reactions measuring approximately 10 mm in diameter 10 h after injection. For foetal rat skin fibroblasts, M. arthritidis ISR-1 membranes were toxic in concentrations from 1 micrograms (3H-uridine incorporation), 10 micrograms (14C-leucine incorporation) and 100 micrograms (3H-thymidine incorporation) of membrane protein.
D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000633:toxicity*; D001427:Bacterial Toxins / Q000008:administration & dosage / Q000633:toxicity*; D002642:Chick Embryo; D005260:Female; D008565:Membrane Proteins / Q000008:administration & dosage / Q000633:toxicity*; D051379:Mice; D009174:Mycoplasma / Q000472:pathogenicity*; D051381:Rats; D011919:Rats, Inbred Strains; D012772:Shock, Septic / Q000209:etiology
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D008565:Membrane Proteins