Cellular location of rat muscle ferritins and their preferential loss during cell isolation.
Heart and other muscles of the rat contain two forms of ferritin separable in polyacrylamide gel electrophoresis. The cellular location of the fast- and slow-migrating ferritins was investigated using primary cultures of hindlimb skeletal muscle, and isolated myocardial cell populations. Muscle and non-muscle cells were isolated in good yield from hearts of adult rats pretreated with large doses of iron to increase their ferritin content. In virtually all cases, the isolated muscle cells contained traces only of the fast-migrating species and the non-muscle cells contained small amounts of the slow-migrating ferritin. During cell isolation, 90-100% of both ferritins was lost and could be recovered in the perfusates and solutions employed, while one third of the total tissue protein, and a larger percentage of creatine phosphokinase, was recovered in the isolated cells. Primary cultures of thigh muscle from adult rats which had differentiated into multi-nucleated myotubes, were incubated for 1-3 days with chelated iron. These cells contained substantial amounts of the electrophoretically fast migrating ferritin, with its characteristic larger Stokes' radius (determined by quantitative polyacrylamide gel electrophoresis). None of the slow-migrating ferritin species was detected, although hindlimb muscle from iron-treated rats contained both forms. It is concluded that the fast-migrating ferritin of muscle, which is much larger and more asymmetric than other ferritins, is confined to the muscle cell population, while the other form is predominantly or exclusively in the non-muscle cells. Both ferritins are lost preferentially over other proteins during procedures which injure muscle tissue.
D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000032:analysis; D005260:Female; D005293:Ferritins / Q000032:analysis*; D006321:Heart / Q000502:physiology*; D009132:Muscles / Q000166:cytology / Q000502:physiology*; D009206:Myocardium / Q000166:cytology; D009211:Myoglobin / Q000032:analysis; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D009211:Myoglobin; D005293:Ferritins; D003402:Creatine Kinase