Effect of vehicle on elicitation of DNCB contact allergy in the guinea pig.
22 DNCB sensitized guinea pigs were challenged with varying amounts dissolved in alcohol, acetone and olive oil. DNCB applied in alcohol resulted in almost 100% positive reactions; the test scores correlated to dose. When similar amounts were applied in alcohol and acetone, the former produced a significantly higher degree of positivity. The importance of defining allergen concentration, volume or weight of test substance applied and test area size, when comparing test results in guinea pigs and humans, is emphasized.
D000818:Animals; D003877:Dermatitis, Contact / Q000209:etiology*; D004137:Dinitrochlorobenzene / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006168:Guinea Pigs; D009578:Nitrobenzenes / Q000633:toxicity*; D010328:Patch Tests; D014677:Pharmaceutical Vehicles
D004137:Dinitrochlorobenzene; D009578:Nitrobenzenes; D014677:Pharmaceutical Vehicles