Phosphorylation in vivo of rat hepatic glucocorticoid receptor.
Rat liver glucocorticoid receptors were labeled in vivo with [32P]orthophosphate. In the last two fractionation procedures leading to purified, molybdate-stabilized, unactivated receptor complex, bound [32P] coeluted with peaks of bound [3H]triamcinolone acetonide. SDS-gel electrophoresis revealed [32P] labeled 90K and 24K bands. The lower molecular weight band is heavily phosphorylated and it could be either a component of the unactivated receptor or a degradation product.
D000818:Animals; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000378:metabolism*; D008297:Male; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000302:isolation & purification / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid