Phalloidin depletes the mitochondrial Ca2+ compartment of hepatocytes.
In 3 h of incubation, primary cultures of rat hepatocytes attach to the substratum and exchange about 2.2 nmol 45Ca2+ per mg protein. In the presence of 1 microM phalloidin, the exchanged amount of 45Ca2+ was found to be decreased by about 30%. Using the uncoupling agent FCCP and the ionophore A23187 for further characterisation we determined that the 45Ca2+ deficit caused by phalloidin occurs in the FCCP-sensitive compartment, i.e., the mitochondria.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002451:Cell Compartmentation / Q000187:drug effects; D002478:Cells, Cultured; D008099:Liver / Q000187:drug effects*; D008930:Mitochondria, Liver / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology*; D010590:Phalloidine / Q000494:pharmacology*; D051381:Rats
D009842:Oligopeptides; D010590:Phalloidine; D002118:Calcium