Application of high performance liquid chromatography to the analysis of cytoplasmic and nuclear oestrogen receptors.
Soluble and nuclear preparations of fresh human endometrium have been analysed by HPLC on a gel permeation column. With high ionic strength eluants recoveries of receptor binding were less than 40% while low ionic strength eluants gave recoveries of 70% or greater. Non-specific binding by endometrial and plasma proteins was largely lost at both high and low ionic strength. Soluble binding components saturable by diethylstilboestrol (DES) were resolved into two components differing in heat lability and response to variations in ionic strength. Molybdate appears to alter the receptor protein rather than the activity of inactivating enzymes. At low ionic strength nuclear binding components saturable by DES could be resolved into two components.
D002467:Cell Nucleus / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003593:Cytoplasm / Q000032:analysis; D004054:Diethylstilbestrol / Q000378:metabolism; D004717:Endometrium / Q000032:analysis; D005260:Female; D006801:Humans; D009994:Osmolar Concentration; D011960:Receptors, Estrogen / Q000032:analysis*
D011960:Receptors, Estrogen; D004054:Diethylstilbestrol