Transmission electron microscopy of heart and liver tissues from rats fed with gums arabic and tragacanth.
Transmission electron microscopy has been used to examine the ultrastructure of rat hearts and livers after diet supplementation with (a) 0, 0.5, 1.5, 2.5 and 3.5% (w/w) gum tragacanth (GT) for 91 days, (b) 0 and 1% GT for 5 days (c) 0, 1, 4 and 8% (w/w) gum arabic (GA) for 28 days. The preparation and scrutiny of the electron micrographs was undertaken by two independent teams of specialists. There were no detectable abnormalities in any of the organelles in the heart and liver specimens from any of the test animals and no inclusions nor other pathological changes were observed. All micrographs showed normal, healthy tissues; particular attention was given to the mitochondria in hepatocytes as they serve as sensitive indicators of the health and state of activity of cells. In addition, the data obtained from assays of the microsomal protein and cytochrome P-450 content of the livers showed that GA and GT did not cause inductive effects. These results do not support earlier suggestions, based on in vitro assays, that GA and GT cause changes in the function of rat heart and liver mitochondria and liver microsomes; however, they confirm a report by Zbinden that the ingestion of GT does not produce abnormalities in the cardiac function of rats.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D006170:Gum Arabic / Q000008:administration & dosage / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D008862:Microsomes, Liver / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects; D009206:Myocardium / Q000648:ultrastructure*; D011134:Polysaccharides / Q000633:toxicity*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014144:Tragacanth / Q000008:administration & dosage / Q000633:toxicity*
D011134:Polysaccharides; D011506:Proteins; D006170:Gum Arabic; D014144:Tragacanth; D003577:Cytochrome P-450 Enzyme System