Experience with a simple high-performance liquid chromatography method for the analysis of purine and pyrimidine nucleosides and bases in biological fluids.
Purine and pyrimidine nucleosides and bases were analysed using an isocratic reverse-phase HPLC technique and utilising simple sample preparation. The method has been successfully used to separate a large number of biologically occurring nucleic acid products within a 30-minute period and to identify them by their chromatographic and optical properties. A number of metabolic disorders have been identified, including hypoxanthine-guanine phosphoribosyl transferase and ornithine carbamyl transferase deficiencies. The method has been in routine use for 2 years performing both quantitative and qualitative analysis and has proved to be robust and reliable.
D001238:Asphyxia Neonatorum / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007926:Lesch-Nyhan Syndrome / Q000378:metabolism; D020163:Ornithine Carbamoyltransferase Deficiency Disease; D011684:Purine Nucleosides / Q000032:analysis* / Q000097:blood / Q000652:urine; D011687:Purines / Q000032:analysis* / Q000097:blood / Q000652:urine; D011741:Pyrimidine Nucleosides / Q000032:analysis* / Q000097:blood / Q000652:urine; D011743:Pyrimidines / Q000032:analysis* / Q000097:blood / Q000652:urine; D014970:Xanthines / Q000032:analysis
D011684:Purine Nucleosides; D011687:Purines; D011741:Pyrimidine Nucleosides; D011743:Pyrimidines; D014970:Xanthines