Electronic counting of spinal fluid cells.
An analysis of the electronic counting of leukocytes in cerebrospinal fluid (CSFLpc) was made theoretically in models and in patients. At spinal fluid dilutions of 1/500, 1/50, 1/25, and 1/2, linearity was obtained by electronic counting down to 1,000, 100, 20, and 2 Lpc (10(6)/L), respectively. The electronic particle counters produce satisfactory results at Lpc greater than 100 (10(6)/L) but need modifications to produce satisfactory results at Lpc less than 100 (10(6)/L). It is theoretically possible to reduce the variation nine times by electronic counting as compared with microscopic counting. A method for the correction of blood admixture at traumatic spinal puncture by electronic counting was shown to be satisfactory.
D002555:Cerebrospinal Fluid / Q000166:cytology*; D004582:Electronics, Medical / Q000379:methods; D006801:Humans; D008853:Microscopy / Q000379:methods; D008954:Models, Biological; D013129:Spinal Puncture