Analysis of MOPC-315 plasmacytoma by elutriation and flow cytometry.
Intravenously transplanted murine plasmacytoma MOPC-315 cells were separated from normal spleen cells from a tumour-bearing mouse by elutriation and characterized according to morphology, immunologic properties and clonogenicity. Morphologically, both lymphocytoid and plasmacytoid cells were separable by elutriation. Flow cytometry correlated DNA content and intracytoplasmic IgA content and demonstrated two distinct populations, both in cell cycle, but with markedly different cellular IgA levels. Density gradient separation characterized the lower-density cells with lower IgA content and higher clonogenicity. From these studies a model of cellular differentiation is proposed.
D000818:Animals; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D002999:Clone Cells / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D007070:Immunoglobulin A / Q000032:analysis; D051379:Mice; D010954:Plasmacytoma / Q000276:immunology / Q000473:pathology*; D012397:Rosette Formation
D004273:DNA, Neoplasm; D007070:Immunoglobulin A