llC labelling of a protein: concanavalin A.
A method is described for the 11C labelling of a phytoagglutinin, concanavalin A, involving reductive methylation by formaldehyde and sodium cyanoborohydride. The reaction mixture is then chromatographed by gel permeation. The quantities (20-80 mCi) and specific activities (20-170 mCi/mg) obtained are such that a practical application of the method is possible. No evidence was found, however, of any preferential uptake of concanavalin A by Krebs II ascite cells in Swiss mice.
D000818:Animals; D002250:Carbon Radioisotopes; D002287:Carcinoma, Krebs 2 / Q000378:metabolism; D003208:Concanavalin A / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods; D051379:Mice; D009368:Neoplasm Transplantation
D002250:Carbon Radioisotopes; D003208:Concanavalin A