title: High-performance liquid chromatographic analysis of neomycin in petrolatum-based ointments and in veterinary formulations.
abstract: A high-performance liquid chromatographic (HPLC) method has been developed for the assay of neomycin in petrolatum-based ophthalmic and topical ointments and in veterinary formulations. Neomycin assay interferences from drugs, such as bacitracin and polymyxin B and inactive components, e.g. wax, were eliminated by a methanol wash and/or a partitioning method. The extracted neomycin was derivatized with 2,4-dinitrofluorobenzene followed by normal-phase HPLC with detection at 254 nm. The average recovery of neomycin from spiked samples was approximately 100% with a relative standard deviation of less than 1%.
mesh_terms: D001414:Bacitracin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D009355:Neomycin / Q000032:analysis*; D009824:Ointments / Q000032:analysis; D010577:Petrolatum; D011112:Polymyxin B / Q000032:analysis; D013256:Steroids / Q000032:analysis; D014730:Veterinary Medicine
chemical_list: D004338:Drug Combinations; D009824:Ointments; D013256:Steroids; D001414:Bacitracin; D010577:Petrolatum; D009355:Neomycin; D011112:Polymyxin B