[Isolation of oligonucleotides from partial hydrolysates of DNA using template chromatography].
Purine oligonucleotides are adsorbed at 0 degree C on poly(vinyl alcohol)-p(dC)n-DEAE-cellulose, and pyrimidine oligonucleotides on oligo(guanylic acid) gel according to the base-pairing mechanism, if their sequences contain at least three or more homologous, consecutive guanylic or cytidylic moieties. By increasing the temperature all base-paired oligonucleotides are desorbed. With this template chromatography mixtures of defined purine- or pyrimidine oligonucleotides could be isolated from fractionated partial hydrolyzates of herring sperm DNA. Afterwards these mixtures are rechromatographed on QAE-Sephadex and/or Nucleosil C18. Using this approach oligonucleotides up to nine monomer units can be isolated either as single substances or as mixtures with defined composition on a preparative scale, which would be not possible with the already existing separation procedures when partial hydrolyzates of herring sperm DNA as starting material are used. Purity and sequence of the isolated oligonucleotides are determined by the "fingerprint" method.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004247:DNA; D005399:Fishes; D006868:Hydrolysis; D008297:Male; D009838:Oligodeoxyribonucleotides / Q000302:isolation & purification*; D009841:Oligonucleotides / Q000302:isolation & purification*; D013094:Spermatozoa; D013698:Templates, Genetic
D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D004247:DNA
Isolierung von Oligonucleotiden aus DNA-partialhydrolysaten mit Hilfe der Template-Chromatographie.