An efficient method for labelling antibodies with 111In.
Using a new method, rabbit IgG and a monoclonal antibody have been conjugated with the chelating agent DTPA. This was accomplished with reaction conditions that should entail lower antibody damage than existing methods. Gel filtration of the 111In-labelled antibody conjugate indicated minimal damage to the antibody and radioimmunoassay showed no significant change in its immunological activity.
D000906:Antibodies; D002614:Chelating Agents; D055598:Chemical Phenomena; D002621:Chemistry; D007204:Indium; D004369:Pentetic Acid; D011863:Radioimmunoassay / Q000379:methods*; D011868:Radioisotopes
D000906:Antibodies; D002614:Chelating Agents; D011868:Radioisotopes; D007204:Indium; D004369:Pentetic Acid