Possible influence of glutathione on postischemic liver injury.
The effect of the free radical scavenger glutathione (GSH) on the early post-ischemic liver cell death was studied on liver tissue of the rat. Animals with different pre-ischemic liver GSH contents were subjected to a 90 min period of ischemia, followed by a 3 h period of reperfusion. Cell death was evaluated morphologically by estimating intracellular calcium, using the stain Alizarin red S (ARS), and by dye-exclusion test using Evans blue. The extent of post-ischemic injury was also assessed by registration of the membrane potential (MP) in liver cells. Four groups of animals were studied: 1) fed rats. 2) fed rats pretreated with diethylmaleate. 3) rats fasted for 48 h. 4) fasted rats pretreated with cobalt-chloride. It was found that the early post-ischemic cell death was more extensive in rats with low initial GSH content (group 2 and 3), than in rats with high GSH content (group 1 and 4). It is suggested that GSH is protective against post-ischemic injury, probably by reducing lipid peroxidation.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D005215:Fasting; D005978:Glutathione / Q000032:analysis / Q000494:pharmacology*; D007511:Ischemia / Q000378:metabolism*; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000032:analysis / Q000098:blood supply* / Q000187:drug effects*; D008112:Liver Glycogen / Q000032:analysis; D008297:Male; D008298:Maleates / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D008055:Lipids; D008112:Liver Glycogen; D008298:Maleates; C014476:diethyl maleate; D005978:Glutathione