Mitochondrial and cytosolic glutathione after depletion by phorone in isolated hepatocytes.
The glutathione content of cytosol and mitochondria of isolated hepatocytes was depleted by addition of a low concentration of phorone (0.5 mM) by 75% and 40% respectively. Different rates of replenishment indicate metabolic separation of cytosolic and mitochondrial glutathione pools. The release from hepatocytes occurred at a rate of about 8 nmol/g wet weight/min, both in controls and after phorone depletion.
D000818:Animals; D003600:Cytosol / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D007659:Ketones / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000648:ultrastructure*; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007659:Ketones; C018637:phorone; D005978:Glutathione