Absence of antibodies to ethinyl estradiol in users of oral contraceptive steroids.
Sera from current and past oral contraceptive users, never-users, and never-users with venous thrombosis were examined for immunoglobulin G (IgG) which was precipitable at 25% or 33% saturation with ammonium sulfate or which showed specific binding of ethinyl estradiol. No increase in "specific" IgG and no evidence of ethinyl estradiol binding was observed in oral contraceptive users. This provides no support for the claim that a significant percentage of oral contraceptive users develop a special IgG, precipitable at 25% ammonium sulfate saturation and showing high binding affinity to ethinyl estradiol, which may be causally linked to the development of thrombotic phenomena in oral contraceptive users.
D000906:Antibodies / Q000032:analysis*; D000936:Antigen-Antibody Complex / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003276:Contraceptives, Oral; D003278:Contraceptives, Oral, Hormonal; D004997:Ethinyl Estradiol / Q000032:analysis / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D009391:Nephelometry and Turbidimetry
D000906:Antibodies; D000936:Antigen-Antibody Complex; D003276:Contraceptives, Oral; D003278:Contraceptives, Oral, Hormonal; D007074:Immunoglobulin G; D004997:Ethinyl Estradiol