Cytochemical detection of hyaluronidase activity in single human and mouse sperm by an improved substrate-film technique.
A substrate-film method is described that allows the detection of hyaluronidase activity in nearly 100% of single human and mouse sperm. The level of hyaluronidase activity as determined by halo diameters was greater in mouse than in human sperm. This simple method may have use as a screening method for identifying compounds that cause developmental or genetic defects in male germ cells, or for the diagnosis of infertility due to decreased hyaluronidase activity.
D000818:Animals; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000378:metabolism*; D008297:Male; D008722:Methods; D051379:Mice; D013094:Spermatozoa / Q000201:enzymology*; D013997:Time Factors
D006821:Hyaluronoglucosaminidase