The synthesis of 5S RNA and transfer RNA in sea urchin embryos animalized by Evans blue.
Animalization (ectodermalization) of embryos of Arbacia punctulata was induced by continuous treatment from the 2-cell stage with Evans Blue. Quantitative measurements of rates of accumulation of 5S RNA and tRNA in mesenchyme blastulae, in plutei and in corresponding animalized embryos showed that these rates per embryo and per cell were similar in control and in animalized embryos. The alterations in normal cell interactions and germ layer formation induced by Evans Blue did not affect the synthesis of 5S RNA and tRNA in sea urchin embryos.
D000818:Animals; D004475:Ectoderm / Q000187:drug effects / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism; D005070:Evans Blue / Q000494:pharmacology; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D012343:RNA, Transfer / Q000096:biosynthesis*; D012617:Sea Urchins / Q000196:embryology*
D012335:RNA, Ribosomal; D005070:Evans Blue; D012343:RNA, Transfer