[A radioimmunoassay for the determination of human myoglobin: lower detection limit, precision and use in control of myoglobinuric kidney failure].
The lower detection limit of a radioimmunoassay for human myoglobin was determined by two "precision from day to day" methods (90% and 3 s methods), and by two "precision in series" methods (95% confidence range and the method of Markowetz & Munz). According to Markowetz & Munz, the lower detection limit (as a measure of sensitivity) is the lowest myoglobin concentration which, in 15-fold assays, shows no binding value in common with that of the next highest concentration, and no activity value in common with that of the reference binding value. On theoretical grounds (precision in series) and from a practical standpoint (determination of the lower detection limit using one sample assay), this method is the most suitable for the determination of the lower detection limit as a measure of sensitivity. It remains to be seen whether this is a valid generalization for other methods.
D003404:Creatinine / Q000652:urine; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism*; D009211:Myoglobin / Q000032:analysis*; D009212:Myoglobinuria / Q000378:metabolism*; D011863:Radioimmunoassay / Q000379:methods; D012206:Rhabdomyolysis / Q000378:metabolism*
D009211:Myoglobin; D003404:Creatinine
Radioimmuntest zur Bestimmung von Humanmyoglobin: Untere Nachweisgrenze, Präzision, Verlaufskontrolle beim myoglobinurischen Nierenversagen.