Induction of the blue form of bacteriorhodopsin by low concentrations of sodium dodecyl sulfate.
The effects produced on bacteriorhodopsin by low concentrations of several detergents have been studied by absorption and fourth-derivative spectrophotometry. Sodium dodecyl sulfate induces the appearance of the blue form of bacteriorhodopsin (lambda max = 600 nm) at pH values up to 7.0 in a reversible manner. The apparent pK of the purple-to-blue transition raised with increasing concentration of SDS. Of the other detergents tested, only sodium dodecyl-N-sarcosinate showed a slight red-shift of the absorption band to 580 nm, whereas sodium taurocholate, Triton X-100 and cetyltrimethylammonium bromide did not favour the appearance of the blue form. The effect of SDS was found to be consistent with a localized conformational change that moves away the counter-ion of the protonated Schiff base.
D001436:Bacteriorhodopsins / Q000528:radiation effects; D002338:Carotenoids / Q000528:radiation effects; D006217:Halobacterium; D006863:Hydrogen-Ion Concentration; D008027:Light; D012967:Sodium Dodecyl Sulfate; D013057:Spectrum Analysis
D002338:Carotenoids; D012967:Sodium Dodecyl Sulfate; D001436:Bacteriorhodopsins