Small bowel transplantation in the rat: the adverse effect of increased pressure during the flushing procedure of the graft.
The influence of the pressure during the flushing procedure on the results of the subsequent transplantation was investigated in a model of heterotopic functional small bowel transplantation in the outbred Wistar rat. In this model insufficient small bowel preservation will result in a lethal shock, soon after revascularization. If the size of the small bowel graft was reduced to a 15-cm segment of the proximal jejunum, improper preservation did not lead to lethal shock, but to histological changes of the graft. The influence of high flushing pressure (80 cm), medium flushing pressure (50 cm), low flushing pressure (35 cm), and no flushing procedure at all, was investigated in different groups of rats. Flushing was performed by gravity using chilled Ringer's lactate solution (4 degrees C), buffered with NaHCO3 at a pH of 7.4. The results were evaluated histologically as well as clinically. Results indicated flushing of the graft to be superior to a nonflushing procedure. A flushing pressure of 80 cm and even 50 cm proved to be harmful to the jejunal graft. Flushing pressure of 35 cm resulted in successful segmental (jejunal) and subtotal (minus 5- to 7-cm distal ileum) small bowel transplantation. However, survival of a total small bowel graft could not be achieved with the optimal flushing procedure indicating that the functional integrity of a small bowel graft also depends on conditions other than the method of short-term preservation.
D000818:Animals; D006085:Graft Survival; D007421:Intestine, Small / Q000473:pathology / Q000637:transplantation*; D007583:Jejunum / Q000473:pathology / Q000637:transplantation; D008297:Male; D009336:Necrosis; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion / Q000379:methods; D011312:Pressure / Q000009:adverse effects; D051381:Rats; D011919:Rats, Inbred Strains; D012769:Shock / Q000209:etiology