title: Decreased oxidation of labeled glucose by dissociated brain cells in the presence of fetal bovine serum.
abstract: The effect of serum on the rate of substrate oxidation by dissociated brain cells in vitro was examined. At a serum protein concentration of approximately 0.55 milligram per milliliter, oxidation of [6-14C]glucose to 14CO2 was decreased more than 50 percent. Oxidation of [3-14C]-3-hydroxybutyrate and [U-14C]glutamine was decreased much less. Serum from cows, rats, horses, and humans produced similar effects, as did serum from young and old animals and from both sexes. The effect on [6-14C]glucose oxidation was proportional to serum protein concentration, and significant inhibitory activity was obtained with dialyzed serum. Heating (80 degrees C for 10 minutes) significantly reduced the inhibitory activity. These results suggest the presence of a factor in serum that can preferentially decrease glucose oxidation. Such a factor would have profound implications for metabolic regulation in vivo and for studies of cells in vitro in which serum is included in the growth medium.
mesh_terms: D000818:Animals; D001769:Blood; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media; D005947:Glucose / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism; D006885:Hydroxybutyrates / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D003470:Culture Media; D006885:Hydroxybutyrates; D005973:Glutamine; D005947:Glucose