Detection of lipid transfer protein activity in rabbit liver perfusate.
Cholesteryl ester, triacylglycerol and phospholipid transfer activity was detected in rabbit liver perfusate after 2 h perfusions in situ. Lipoproteins were removed from the perfusate plasma by ultracentrifugation prior to hydrophobic interaction chromatography of the lipoprotein-free perfusate. The hydrophobic protein, eluted with water from a Phenyl-Sepharose column, facilitated the transfer of radiolabelled cholesteryl ester, triacylglycerol and phosphatidylcholine from low-density lipoprotein to high-density lipoprotein during 3 h incubations at 37 degrees C. These results suggest that rabbit plasma lipid transfer protein is produced by the liver.
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002788:Cholesterol Esters / Q000378:metabolism; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D010477:Perfusion; D011817:Rabbits
D002352:Carrier Proteins; D002788:Cholesterol Esters