Monoclonal antibodies to a recombinant human leukocyte interferon (rIFN-alpha B).
Recombinant human leukocyte B interferon (rIFN-alpha B) was purified to approximately 60% on a column of monoclonal antibody to IFN-alpha. This partially purified rIFN-alpha B was in turn used as immunogen in obtaining four monoclonal antibodies to this recombinant interferon. All four antibodies, while showing varying degrees of cross-reactivity to some other rIFN-alpha subtypes, showed specificity to monomeric and dimeric forms of rIFN-alpha B only and not to the contaminants in the partially purified preparation as demonstrated in the Western blot immunoassay.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D000918:Antibody Specificity; D001202:Ascitic Fluid / Q000276:immunology; D001345:Autoradiography; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007370:Interferon Type I / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight
D000911:Antibodies, Monoclonal; D007370:Interferon Type I