Direct evidence of incorporation of 12-O-[20-2H1]tetradecanoylphorbol-13-acetate into artificial membranes as determined by deuterium magnetic resonance.
In order to elucidate the manner of interaction of 12-O-tetradecanoylphorbol-13-acetate (TPA), 2H-NMR spectra of [20-2H1]TPA and 31P-NMR spectra were recorded in the presence of multibilayers of dimyristoylphosphatidylcholine (DMPC). Observation of several pairs of quadrupole splittings directly proves the TPA molecules are intercalated into the multibilayers. Further, reduction of the 31P chemical shift anisotropy of DMPC multibilayers by TPA is more pronounced than that of phorbol 12,13-diacetate (PDA) whose activity of tumor promotion is very weak.
D002462:Cell Membrane / Q000378:metabolism*; D003903:Deuterium; D004134:Dimyristoylphosphatidylcholine / Q000138:chemical synthesis; D008051:Lipid Bilayers / Q000138:chemical synthesis; D009682:Magnetic Resonance Spectroscopy; D008567:Membranes, Artificial; D010703:Phorbol Esters / Q000032:analysis; D010704:Phorbols / Q000032:analysis*; D013755:Tetradecanoylphorbol Acetate / Q000032:analysis*
D008051:Lipid Bilayers; D008567:Membranes, Artificial; D010703:Phorbol Esters; D010704:Phorbols; C008874:phorbol-12,13-diacetate; D003903:Deuterium; D013755:Tetradecanoylphorbol Acetate; D004134:Dimyristoylphosphatidylcholine