High-level iodination of monoclonal antibody fragments for radiotherapy.
Two different murine monoclonal antibody Fab fragments specific for p97, a melanoma-associated antigen, were labeled with I-131 at high activity levels without excessive chemical damage. Up to 20 mg of Fab were labeled with up to 300 mCi of I-131 using the chloramine-T method and large working volumes at room temperature. As much as 90% of the initial activity was recovered as labeled product. The labeled Fabs varied in their sensitivity to radioiodination damage, as measured by an in vitro cell-binding assay. Radioiodination was performed safely using a remote iodination apparatus. The final product was of radiopharmaceutical quality suitable for clinical diagnosis and experimental radiotherapy in humans.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000032:analysis*; D000951:Antigens, Neoplasm; D002700:Chloramines; D004781:Environmental Exposure; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000032:analysis*; D007457:Iodine Radioisotopes / Q000627:therapeutic use*; D007553:Isotope Labeling / Q000379:methods; D008545:Melanoma / Q000276:immunology / Q000532:radiotherapy*; D058950:Melanoma-Specific Antigens; D051379:Mice; D009363:Neoplasm Proteins / Q000032:analysis*; D011829:Radiation Dosage; D014105:Tosyl Compounds
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D002700:Chloramines; D007140:Immunoglobulin Fab Fragments; D007457:Iodine Radioisotopes; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D014105:Tosyl Compounds; C016300:chloramine-T