Multitest CMI for standardized measurement of delayed cutaneous hypersensitivity and cell-mediated immunity. Normal values and proposed scoring system for healthy adults in the U.S.A.
The Multitest CMI system, a disposable device that simultaneously applies seven standardized preloaded antigens and diluent control, is a major advance for measurement of delayed type hypersensitivity (DTH) in assessment of cell-mediated immunity (CMI). The system was tested in 402 healthy adults, aged 17 to 92 years, to determine normal values for incidence and size of DTH responses. Incidence of positive responses to individual antigens varied from 85% to 46%, with great variability related to age and sex. To better assess CMI, a two-part score based on 48-hour readings was employed. The mean number of positive antigens ranged between four and five, and the mean sum of their mm induration ranged between 18 and 25, with both scores increasing with advancing age. A statistical zone of reduced DTH scores (hypoergy) was identified. The Multitest CMI system appears to be a practical means of reproducibly assessing CMI in subjects with immunologic, metabolic, infectious, or neoplastic disorders. The scores in our population may serve as reference values to which results from any tested adult can be compared.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging; D000941:Antigens / Q000008:administration & dosage / Q000592:standards*; D005260:Female; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000175:diagnosis* / Q000453:epidemiology / Q000503:physiopathology; D007111:Immunity, Cellular; D007153:Immunologic Deficiency Syndromes / Q000175:diagnosis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D012882:Skin Tests / Q000295:instrumentation* / Q000379:methods; D014481:United States
D000941:Antigens