Characterization and quantification of epidermal and dermal glucocorticoid receptors in the rat.
The interaction between [3H]triamcinolone acetonide and cytosol proteins was studied in separated epidermis and dermis of neonatal rats. Both tissues possessed a binding site with a high affinity (dissociation constant = 0.2 nM) and a low capacity (150-220 fmol/mg protein) for triamcinolone acetonide. The binding site in each tissue cytosol was inactivated by heating at 37 degrees C or by incubating with either trypsin, Triton X-100 or NaCl. The second-order rate constant of association was 1.99 X 10(6)M-1min-1 for the epidermal, and 1.65 X 10(6)M-1min-1 for the dermal receptor binding site. Quantitatively there was a difference between the two tissues with 82% of the receptor being found in the dermis and 18% in the epidermis.
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D004355:Drug Stability; D004817:Epidermis / Q000032:analysis*; D005260:Female; D006358:Hot Temperature; D008297:Male; D017830:Octoxynol; D011092:Polyethylene Glycols; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000032:analysis* / Q000378:metabolism; D011987:Receptors, Steroid / Q000032:analysis*; D012867:Skin / Q000032:analysis*; D012965:Sodium Chloride; D014222:Triamcinolone Acetonide / Q000378:metabolism; D014357:Trypsin
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D017830:Octoxynol; D014357:Trypsin; D014222:Triamcinolone Acetonide