Chromatographic profiling of urinary volatile and organic acid metabolites of normal and diabetic C57BL/Ks mice.
Capillary gas chromatography and gas chromatography-mass spectrometry were used to examine the urinary volatile and organic acid metabolites in normal and diabetic C57BL/Ks male mice. Quantitative differences in the excretion of these metabolites were assessed in the animals from 5 to 24 weeks of age. Statistically significant differences were examined with respect to known metabolic abnormalities in the diabetic animals and to the possible toxic effects of elevated levels of certain metabolites. A number of aldehyde metabolites and aromatic acids, as well as most other organic acids, were found at consistently higher levels in diabetic urine. Several ketone metabolites, linalool, and 2-sec.-butylthiazoline were found at consistently low levels throughout the study.
D000375:Aging; D000447:Aldehydes / Q000652:urine; D000818:Animals; D002264:Carboxylic Acids / Q000652:urine*; D002849:Chromatography, Gas / Q000379:methods; D003921:Diabetes Mellitus, Experimental / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D005947:Glucose / Q000378:metabolism; D007659:Ketones / Q000652:urine; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
D000447:Aldehydes; D002264:Carboxylic Acids; D007659:Ketones; D005947:Glucose