Hybridization and isolation of human-rat beta gamma enolase.
A human-rat hybrid form of enolase (beta gamma) was prepared by dissociation and reassociation of a mixture of human beta beta and rat gamma gamma enolases, followed by isolation of the hybrid form from the parental homodimeric enolases with DEAE-Sephadex column chromatography. The human-rat beta gamma enolase had a specific activity similar to those of human beta beta and rat gamma gamma enolases. The optimal pH, stability against heating, and Km for 2-phosphoglycerate of the hybrid enolase were also similar to those of the homodimeric enolases.
D000818:Animals; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D046911:Macromolecular Substances; D010751:Phosphopyruvate Hydratase / Q000276:immunology / Q000302:isolation & purification; D011489:Protein Denaturation; D051381:Rats; D013045:Species Specificity; D013329:Structure-Activity Relationship
D046911:Macromolecular Substances; D010751:Phosphopyruvate Hydratase