Interaction of T-2 toxin with murine lymphocytes.
T-2 toxin is taken up by lymphocytes in 10-15 min in a saturable manner. Uptake is dependent on temperature and partially on the availability of energy. Approx. 10(5) molecules of T-2 toxin are bound per cell, having a mean affinity constant, Ka = 1.6 X 10(7) M-1. The toxin is rapidly dissociated from the cell to leave approx. 10-15% of the original loading in 1 h. It is concluded that T-2 toxin uptake and release do not follow conventional mechanisms.
D000818:Animals; D004734:Energy Metabolism; D007700:Kinetics; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D012717:Sesquiterpenes / Q000378:metabolism*; D013605:T-2 Toxin / Q000378:metabolism*; D013696:Temperature; D014316:Tritium
D012717:Sesquiterpenes; D014316:Tritium; D013605:T-2 Toxin