Cross-reactive idiotypic reactions between hybridoma proteins with or without anti-peroxidase antibody function.
Hybridomas from lymph node cells of a mouse C57BL/6 immunized with peroxidase were prepared. Two hybridoma proteins, one with and one without reactivity towards peroxidase, were selected and used to prepare antiidiotypic antisera in rabbits. The idiotypic cross-reactions between these two hybridoma proteins were studied using these antiidiotypic antisera. The results obtained indicate that at least two main idiotopes are present on the non-peroxidase binding hybridoma protein, only one of which is shared by the peroxidase-binding hybridoma protein.
D000818:Animals; D000917:Antibody Formation; D003429:Cross Reactions; D006735:Horseradish Peroxidase / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009363:Neoplasm Proteins / Q000276:immunology*; D010544:Peroxidases / Q000276:immunology*; D011817:Rabbits
D007130:Immunoglobulin Idiotypes; D009363:Neoplasm Proteins; D006735:Horseradish Peroxidase; D010544:Peroxidases