title: Distribution of apolipoprotein E isoforms between very low and high density lipoproteins of normal subjects and hyperlipidemic patients with special reference to type III hyperlipidemia.
abstract: Serum very low (VLDL) and high density lipoproteins (HDL) from 17 hyperlipidemic patients and 10 normal subjects have been isolated by preparative ultracentrifugation, and the electrophoretic patterns of apolipoprotein E (apo E) isoforms in the lipoproteins have been examined by isoelectric focusing. Type III hyperlipidemia (dyslipoproteinemia) has been suggested to be a disease caused by an abnormal mutant of the apo E-3 isoform. In accordance with this, all patients with type III hyperlipidemia in the present study showed lack of apo E-3 in VLDL. However, all these patients demonstrated a protein zone corresponding to apo E-3 in their HDL fraction. Patients with other types of hyperlipidemia or normal subjects who showed an apo E-4 variant in VLDL had an HDL that lacked apo E-4. The results support the hypothesis that type III hyperlipidemia is due to an abnormal composition of the VLDL particles rather than a result of an abnormal mutant of apo E-3.
mesh_terms: D001053:Apolipoproteins / Q000032:analysis*; D001057:Apolipoproteins E; D006801:Humans; D006952:Hyperlipoproteinemia Type III / Q000097:blood*; D006951:Hyperlipoproteinemias / Q000097:blood; D007123:Immunoelectrophoresis, Two-Dimensional; D007525:Isoelectric Focusing; D008075:Lipoproteins, HDL / Q000097:blood*; D008079:Lipoproteins, VLDL / Q000097:blood*
chemical_list: D001053:Apolipoproteins; D001057:Apolipoproteins E; D008075:Lipoproteins, HDL; D008079:Lipoproteins, VLDL