[Extraction of native preparations of mRNP-particles from the nuclei of kidney bean cells].
A soft technique is suggested for extracting the mRNP-particles from nuclei. It is based on the incubation of nuclei with ATP. Advantages of the technique, as compared with other described in literature, are as follows: contamination-free mRNP preparations without any DNP fragments are more homogeneous in respect to the CsCl gradient buoyant density and a higher percentage of poly(A)+-mRNP in the total quantity of RNP extracted from nuclei.
D001483:Base Sequence; D002467:Cell Nucleus / Q000032:analysis*; D007887:Fabaceae / Q000032:analysis*; D008722:Methods; D010946:Plants, Medicinal; D012261:Ribonucleoproteins / Q000302:isolation & purification*
D012261:Ribonucleoproteins
Poluchenie nativnykh preparatov m-RNP-chastits iz iader kletok semian fasoli.