The crucial role of hypoxia in halothane-induced lipid peroxidation.
Halothane-induced lipid peroxidation in NADPH-reduced liver microsomes from phenobarbital-pretreated male rats was studied under defined steady state oxygen partial pressures (Po2). Under anaerobic conditions, as well as at a Po2 above 10 mm Hg no halothane-induced formation of malondialdehyde was detected. At a Po2 below 10 mm Hg, however, with a maximum near 1 mm Hg oxygen, significant halothane-induced malondialdehyde formation was found. This evidence supports the hypothesis that halothane can induce lipid peroxidation. The Po2 (i) must be low enough to permit the reductive formation of . CF3 CHCl-radicals but (ii), it must be high enough to promote formation of lipid peroxides.
D000818:Animals; D006221:Halothane / Q000494:pharmacology*; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009249:NADP / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology*; D010313:Partial Pressure; D051381:Rats
D008054:Lipid Peroxides; D008315:Malondialdehyde; D009249:NADP; D010100:Oxygen; D006221:Halothane