An enzyme-linked immunosorbent assay for quassin and closely related metabolites.
A microtitration plate enzyme-linked immunosorbent assay has been developed for quassin and closely related seco-triterpenes. The method is much more sensitive than conventional chromatographic techniques and enables the routine analysis of large numbers of samples with a detection limit of 5 ng quassin. Antibody production was elicited by injecting a conjugate of iso-quassinic acid linked to bovine serum albumin into rabbits. Important features of the technique are (a) the use of a double-antibody, noncompetitive enzyme-linked immunosorbent assay for a low-molecular-weight, nonantigenic compound using microtitration plates; and (b) that the initial incubation of samples with primary antiserum can be carried out in buffer containing up to 20% (v/v) methanol with minimal loss of sensitivity. The structural requirements for recognition of the hapten by the antiserum are considered based on the levels of cross-reaction found with a wide range of other quassinoids.
D055598:Chemical Phenomena; D002621:Chemistry; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005900:Glaucarubin / Q000031:analogs & derivatives / Q000032:analysis*; D000432:Methanol; D010616:Phenanthrenes / Q000032:analysis*; D036702:Quassins
D010616:Phenanthrenes; D036702:Quassins; C040913:isoquassinic acid; D005900:Glaucarubin; C010317:quassin; D000432:Methanol