Evidence for the nucleus-centriole association in living cells obtained by ultracentrifugation.
The association of centrioles with the interphase nuclei of L- and PE-cells has been studied using ultracentrifugation of a cell monolayer in a culture medium at 20 and 37 degrees C. Ultracentrifugation at 10 000 to 40 000 gav for 15 to 60 min did not cause any changes in the cell length or in the size of the nucleus, but entailed delocalization of nuclei. The distance between it and the centrioles hardly ever changes. Any nucleus-delocalizing centrifugation of non-treated cells also resulted in the centrioles being shifted towards the centripetal nucleus pole. After 30 to 60 min, at 40 000 gav, the cells remained viable and capable of mitosis. More intensive centrifugation (15 min at 70 000 gav) proved to be fatal to the cells. The distance between the centrioles and the nucleus became greater in the cells which were centrifuged after incubation with cytochalasin B. The results are interpreted as lending support to the previously demonstrated [28] association between the centrioles and the nucleus in the interphase cells.
D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000648:ultrastructure*; D002478:Cells, Cultured; D002502:Centrioles / Q000648:ultrastructure*; D004848:Epithelium / Q000648:ultrastructure; D007399:Interphase; D007668:Kidney / Q000648:ultrastructure; D007739:L Cells / Q000648:ultrastructure; D009940:Organoids / Q000648:ultrastructure*; D013552:Swine; D014461:Ultracentrifugation