[Preparative isolation of tetra-, penta- and hexapurine oligonucleotides from partial hydrolysates of depyrimidinated herring sperm DNA].
Herring sperm DNA is chemically degraded to a complex mixture of purine nucleotides. The oligonucleotides are separated from the partial hydrolysates by column chromatography. The resulting mixture of trimer to hexamer purine oligonucleotides is subsequently fractionated on QAE-Sephadex into different mixtures of sequence-isomeric purine oligonucleotides. In a final separation, which uses reversed-phase (Nucleosil C18) high-performance liquid chromatography, these mixtures are separated under isocratic conditions into 35 pure defined purine oligonucleotides with four to six monomer units, 14 defined mixtures of sequence-isomeric purine oligonucleotides and several unidentified products. Purity and sequence of the isolated oligonucleotides are determined by the "fingerprint" method. The results of the high-performance liquid chromatographic and the "fingerprint" methods of the isolated oligonucleotides are discussed.
D000818:Animals; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D004247:DNA / Q000032:analysis*; D005399:Fishes / Q000378:metabolism*; D006868:Hydrolysis; D008297:Male; D009838:Oligodeoxyribonucleotides / Q000302:isolation & purification*; D009841:Oligonucleotides / Q000302:isolation & purification*; D011685:Purine Nucleotides / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D013094:Spermatozoa / Q000032:analysis*
D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D011685:Purine Nucleotides; D004247:DNA
Präparative Isolierung von Tetra-, Penta- und Hexapurinoligonucleotiden aus Partialhydrolysaten depyrimidinierter Heringsspermen-DNA.