The covalent structure of hepatic microsomal epoxide hydrolase. I. Isolation and characterization of the cyanogen bromide fragments.
Microsomal epoxide hydrolase was purified to homogeneity from phenobarbital-induced rabbit liver for the purpose of determining the complete amino acid sequence. All of the expected 11 cyanogen bromide fragments of epoxide hydrolase were isolated by a combination of gel filtration and high pressure liquid chromatography. Each was characterized by its amino acid composition and NH2-terminal amino acid sequence. The complete amino acid sequences of the eight small fragments, from 5-29 residues, were determined.
D000595:Amino Acid Sequence; D000818:Animals; D003488:Cyanogen Bromide; D004851:Epoxide Hydrolases / Q000032:analysis*; D008862:Microsomes, Liver / Q000201:enzymology*; D010446:Peptide Fragments / Q000302:isolation & purification*; D011817:Rabbits
D010446:Peptide Fragments; D004851:Epoxide Hydrolases; D003488:Cyanogen Bromide