Microbial hydroxylation of novobiocin and related compounds.
A new soil actinomycete (UC 5762, NRRL 11111) was found to transform novobiocin to 11-hydroxynovobiocin. The product was isolated by solvent extraction and column chromatography, and identified by IR, UV, 1H NMR and 13C NMR spectroscopy. Related structures (8,9-dihydronovobiocin, novobiocic acid and chlorobiocin) were similarly transformed to their corresponding C-11 hydroxylated analogues. The microbial process is superior to chemical (selenium dioxide) oxidation which yielded a mixture of 11-hydroxy- and 11-oxonovobiocin.
D000192:Actinomycetales / Q000378:metabolism*; D001711:Biotransformation; D006900:Hydroxylation; D009675:Novobiocin / Q000378:metabolism*
D009675:Novobiocin