[Effective method of isolating M4-lactate dehydrogenase from rat liver].
Lactate dehydrogenase M4-isoform in the homogeneous state was isolated from the rat liver by successive application of sulphate-ammonium fractionation, phosphocellulose ion-exchange chromatography with high-affinity elution of 1 mM NADH and subsequent hydroxyl apatite fractionation. The method permits obtaining the preparation amounts of the enzymic protein with yield 37.5%, specific activity 386.8 units per 1 mg of protein. It is established that 1 mM NAD+, 10 mM pyruvate and 100 mM lactate are also effective as agents of the selective enzyme elution.
D000818:Animals; D002482:Cellulose / Q000031:analogs & derivatives; D002852:Chromatography, Ion Exchange / Q000379:methods; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification*; D008099:Liver / Q000201:enzymology*; D051381:Rats
D007527:Isoenzymes; D002482:Cellulose; C008881:phosphocellulose; D007770:L-Lactate Dehydrogenase
Effektivnyĭ metod vydeleniia M4-laktatdegidrogenazy iz pecheni krysy.