Cytosolic free Ca2+ in isolated rat hepatocytes as measured by quin2. Effects of noradrenaline and vasopressin.
Cytosolic [Ca2+] has been measured by using the Ca2+-sensitive indicator quin2 in rat liver cells. Optimal loading and hydrolysis have been obtained by equilibrating the cells with 50 microM quin2 acetoxymethyl ester for 150 s. The increase in [Ca2+]i initiated by noradrenaline and vasopressin was reduced but not abolished by removing external Ca2+.
D000634:Aminoquinolines; D000818:Animals; D002118:Calcium / Q000032:analysis*; D005456:Fluorescent Dyes; D008099:Liver / Q000032:analysis* / Q000187:drug effects; D009638:Norepinephrine / Q000494:pharmacology*; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D014667:Vasopressins / Q000494:pharmacology*
D000634:Aminoquinolines; D005456:Fluorescent Dyes; D014667:Vasopressins; C035738:Quin2-acetoxymethyl ester; D011433:Propranolol; D002118:Calcium; D009638:Norepinephrine