title: Studies on peptides. CXIII. Synthesis of the heptacosapeptide amide corresponding to the entire amino acid sequence of chicken secretin.
abstract: The heptacosapeptide amide corresponding to the entire amino acid sequence of chicken secretin was synthesized by assembling four peptide fragments followed by deprotection with thioanisole-mediated trifluoromethanesulfonic acid in TFA. The deprotected peptide was purified by gel-filtration on Sephadex G-25, followed by ion-exchange chromatographies on CM-cellulose and DEAE-Sepharose. Synthetic peptide stimulated the flow of pancreatic alkaline juice and decreased systemic blood pressure in rats.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D007202:Indicators and Reagents; D009904:Optical Rotation; D010455:Peptides / Q000138:chemical synthesis*; D012633:Secretin / Q000138:chemical synthesis*
chemical_list: D007202:Indicators and Reagents; D010455:Peptides; D012633:Secretin