Fluorescence polarization and spectropolarimetric studies on the conformational changes induced by omega-aminoacids in two isozymes of Glu-plasminogen (I and II).
Conformational changes of two isozymes of Glu-plasminogen (Glu-plg I and II) induced by omega-aminoacids were studied by using fluorescence polarization and spectropolarimetry. The rotational relaxation times (Pn) of FITC labeled Glu-Plg I and II decreased in the presence of 6 aminohexanoic acid (6AHA) or tranexamic acid (t-x), which may mean increase in Brownian motion of FITC labeled region (possibly N-terminal region) of Glu-plg I and II when 6AHA or t-x binds with lysine binding sites (LBS) of these plasminogens. Glu-plg II seems to have longer rotational relaxation time compared to that of Glu-plg I, which may mean smaller extent of Brownian motion of FITC labeled region of Glu-plg II in comparison to that of Glu-plg I. The far ultraviolet circular dichroism (CD) spectra indicate that there may be some difference in the polypeptide backbone between Glu-plg I and II, possibly more of beta-structure and less of random coil structure in Glu-plg II in comparison to Glu-plg I. The presence of 6AHA or t-x gave rise to larger change of the negative ellipticity at around 208 nm in Glu-plg I in comparison to its change in Glu-plg II, which may mean the larger extent of conformational change of Glu-plg I induced by 6AHA or t-x than that of Glu-plg II.
D000614:Aminocaproates / Q000494:pharmacology*; D015119:Aminocaproic Acid / Q000494:pharmacology*; D003509:Cyclohexanecarboxylic Acids / Q000494:pharmacology*; D005454:Fluorescence Polarization; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D010958:Plasminogen / Q000378:metabolism*; D011487:Protein Conformation / Q000187:drug effects; D013056:Spectrophotometry, Ultraviolet; D014148:Tranexamic Acid / Q000494:pharmacology*
D000614:Aminocaproates; D003509:Cyclohexanecarboxylic Acids; D007527:Isoenzymes; D010455:Peptides; D014148:Tranexamic Acid; D010958:Plasminogen; D015119:Aminocaproic Acid