Multiple forms of aspartate aminotransferase as studied by liquid chromatography.
A convenient method, based on high-performance Liquid chromatography (HPLC) is described for the analysis of multiple forms of cytosolic aspartate aminotransferase. The method has a better resolution; in the same time it is drastically more rapid than the conventional chromatography on CM-Sephadex. The results suggest that transition forms arising from the heat denaturation of the enzyme can be detected by HPLC.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003600:Cytosol / Q000201:enzymology; D007527:Isoenzymes / Q000302:isolation & purification*; D009206:Myocardium / Q000201:enzymology; D013552:Swine; D013696:Temperature
D007527:Isoenzymes; D001219:Aspartate Aminotransferases