Amino acid sequence of human plasma apolipoprotein C-II from normal and hyperlipoproteinemic subjects.
The complete amino acid sequence of human plasma apolipoprotein C-II isolated from normal individuals and a subject with type V hyperlipoproteinemia has been determined. Apo-C-II contains 79 amino acids with the following amino acid composition: Asp4, Asn1, Thr9, Ser9, Glu7, Gln7, Pro4, Gly2, Ala6, Val4, Met2, Ile1, Leu8, Tyr5, Phe2, Lys6, Arg1, Trp1. Cleavage of apo-C-II by cyanogen bromide produced three peptides designated as CB-1 (9 residues), CB-2 (51 residues), and CB-3 (19 residues). Two peptides, CT-1 (50 residues) and CT-2 (29 residues), which overlapped the cyanogen bromide peptides, were obtained by tryptic digestion of citraconylated apo-C-II at the single arginine residue. The amino acid sequence of apo-C-II was obtained by the automated phenyl isothiocyanate degradation of intact apo-C-II and the peptides produced by cleavage of apo-C-II by cyanogen bromide and trypsin. Phenylthiohydantoins were identified by high performance liquid chromatography and chemical ionization-mass spectrometry. The amino acid sequence of apo-C-II from the normal subject was identical with the apo-C-II isolated from the hyperlipoproteinemic subject.
D000595:Amino Acid Sequence; D053304:Apolipoprotein C-II; D001053:Apolipoproteins / Q000097:blood*; D001056:Apolipoproteins C; D002850:Chromatography, Gel; D003488:Cyanogen Bromide; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006954:Hyperlipoproteinemia Type V / Q000097:blood*; D014357:Trypsin / Q000378:metabolism
D053304:Apolipoprotein C-II; D001053:Apolipoproteins; D001056:Apolipoproteins C; D014357:Trypsin; D003488:Cyanogen Bromide