title: Anion requirement and effect of anion transport inhibitors on the response of vero cells to diphtheria toxin and modeccin.
abstract: The anion requirement for toxic action of diphtheria toxin and modeccin was studied. In Cl- -free Hepes buffer made isotonic with mannitol, cells were insensitive to diphtheria toxin and modeccin. Just 2 mM NaCl was sufficient to obtain full toxic activity of modeccin, whereas 140 mM NaCl was required for maximal intoxication with diphtheria toxin. Br- could substitute for Cl-. NO3-,l-, and ClO3- were less efficient than Cl-, whereas SO42- and SCN- were unable to replace Cl- . Cl- deprivation both reduced the ability of cells to bind diphtheria toxin and prevented bound toxin from intoxicating the cells. The binding of modeccin was not reduced. SITS (4-acetamide-4'-isothiocyano-stilbene-2,2'-disulfonic acid), an inhibitor of Cl- entry, protected against diphtheria toxin and modeccin, indicating that Cl- transport is required for intoxication.
mesh_terms: D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000494:pharmacology; D000818:Animals; D000838:Anions / Q000378:metabolism*; D001693:Biological Transport, Active / Q000187:drug effects; D001965:Bromides / Q000494:pharmacology; D002460:Cell Line; D002712:Chlorides / Q000378:metabolism; D002522:Chlorocebus aethiops; D003486:Cyanides / Q000494:pharmacology; D004167:Diphtheria Toxin / Q000494:pharmacology*; D007454:Iodides / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects*; D037102:Lectins / Q000494:pharmacology*; D037121:Plant Lectins; D054790:Ribosome Inactivating Proteins, Type 2
chemical_list: D000838:Anions; D001965:Bromides; D002712:Chlorides; D003486:Cyanides; D004167:Diphtheria Toxin; D007454:Iodides; D037102:Lectins; D037121:Plant Lectins; D054790:Ribosome Inactivating Proteins, Type 2; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; C016004:modeccin