Gossypol induces DNA strand breaks in human fibroblasts and sister chromatid exchanges in human lymphocytes in vitro.
The male contraceptive agent gossypol was found to induce a dose related increase of DNA strand breaks in human fibroblasts in vitro at concentrations of 5 to 40 micrograms/ml. The effect was reduced in the presence of 2% fetal calf serum. A weak but reproducible increase in the SCE frequency was found in human lymphocytes treated for 1 hour in serum-free medium with 0.04 to 4 micrograms/ml of gossypol.
D002478:Cells, Cultured; D003434:Crossing Over, Genetic / Q000187:drug effects*; D004277:DNA, Single-Stranded / Q000032:analysis; D006072:Gossypol / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D009154:Mutation / Q000187:drug effects*; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D004277:DNA, Single-Stranded; D006072:Gossypol