Effect of chlorpromazine in vitro on release of enzymes from lysosomes of the bovine retinal pigment epithelium.
The effects of chlorpromazine on lysosomal enzymes and the release of enzymes from lysosomes of bovine retinal pigment epithelial cells were studied in vitro, using cathepsin D, arylsulfatase, and acid phosphatase as lysosomal marker enzymes. Chlorpromazine had little effect on the enzyme activity of cathepsin D and arylsulfatase and slightly decreased that of acid phosphatase. Chlorpromazine accelerated considerably the release of cathepsin D and arylsulfatase, but only minimally affected the release of acid phosphatase. The release of these enzymes from lysosomes depended on the dose of chlorpromazine.
D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism*; D002402:Cathepsin D; D002403:Cathepsins / Q000378:metabolism*; D002417:Cattle; D002746:Chlorpromazine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008247:Lysosomes / Q000201:enzymology; D010857:Pigment Epithelium of Eye / Q000187:drug effects / Q000201:enzymology*; D013429:Sulfatases / Q000378:metabolism*
D000135:Acid Phosphatase; D013429:Sulfatases; D001192:Arylsulfatases; D002403:Cathepsins; D002402:Cathepsin D; D002746:Chlorpromazine