Nonhistone protein antigen profiles of five leukemic cell lines reflect the extent of myeloid differentiation.
The human leukemic cell lines, K562, KG-1, and HL-60, and the blast subclones, KG-1a and HL-60 blast, were utilized to relate differences in nonhistone protein antigens to stages of myeloid cell differentiation. Chromatin proteins were separated on SDS-polyacrylamide gels, transferred electrophoretically to nitrocellulose sheets, and visualized by the peroxidase-antiperoxidase method of Sternberger. Screening with antisera raised against total and dehistonized chromatin and a nuclear extract from these cells revealed quantitative as well as qualitative differences between the cell lines. A decrease in antigen content seemed to parallel progressive stages of myeloid cell development. The results indicate that a number of chromosomal protein antigens are lost or modified during differentiation. An antigen(s) of approximately 55,000 molecular weight was found in HL-60 chromatin, but was not present in its less differentiated subclone or in the other lines representative of earlier stage cells. Upon the induction of HL-60 cells to mature to end stages with 4 microM retinoic acid, a significant increase in the mol wt 55,000 activity was seen. This antigen was detected only with antisera against HL-60 total chromatin and granulocyte nuclei, and it was found only in normal mature granulocytes and in the later stage cells of the HL-60 culture. Thus, the antigen appears to be associated with a differentiated myeloid function.
D000912:Antibodies, Neoplasm / Q000276:immunology; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology; D034961:Antigens, Nuclear; D001853:Bone Marrow / Q000473:pathology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D002843:Chromatin / Q000276:immunology; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology; D002999:Clone Cells / Q000276:immunology / Q000473:pathology; D006801:Humans; D007938:Leukemia / Q000097:blood / Q000276:immunology* / Q000473:pathology; D009698:Nucleoproteins / Q000235:genetics* / Q000276:immunology; D014212:Tretinoin / Q000494:pharmacology
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D034961:Antigens, Nuclear; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D009698:Nucleoproteins; D014212:Tretinoin