A simple method for high resolution banding of chromosomes in amniotic fluid cells.
A simple technique which results in good quality early mitotic stages of amniotic fluid (AF) cells is presented. Two days after trypsinization AF cell cultures are incubated for 4 h in culture medium containing 20 U/ml liquemin. During the last hour 5 micrograms/ml ethidium bromide (EB) is added and 15 min before harvest 0.04 micrograms/ml colcemid is applied as usual. G-banded and Q-banded chromosomes corresponding to at least 550-850 bands per haploid genome can be obtained in sufficient numbers.
D000653:Amniotic Fluid / Q000166:cytology*; D002478:Cells, Cultured; D002871:Chromosome Banding / Q000379:methods*; D002906:Chromosomes, Human, 6-12 and X; D003078:Colchicine / Q000494:pharmacology; D004996:Ethidium / Q000494:pharmacology; D005260:Female; D006801:Humans; D007621:Karyotyping; D008297:Male; D008938:Mitosis; D011247:Pregnancy; D011296:Prenatal Diagnosis; D011418:Prophase; D014178:Translocation, Genetic
D004996:Ethidium; D003078:Colchicine