Metastasis control by injection of blood lymphocytes cultured with neuraminidase-treated MT/W 449A rat mammary carcinoma.
Inevitable metastatic disease was partially controlled in Wistar/Furth rats after intraperitoneal injection of blood lymphocytes cultured with neuraminidase-treated tumor cells and phytohemagglutinin after amputation of implanted syngeneic MT/W 449A mammary adenocarcinoma. Control of MT/W 449A metastasis was limited to animals with primary tumors weighing less than 700 mg. An optimal 20-25 X 10(6)-lymphocyte dose gave control in 8 of 18 animals (44%). Metastasis control rate was 50% in animals with small primary tumors (less than 250 mg). All animals in different control groups died of metastatic disease, without significant survival differences among overall groups or tumor weight-stratified subgroups. Intraperitoneal injection of neuraminidase-treated tumor cells alone did not control metastatic disease. Cell-mediated 51Cr-release cytotoxicity indexes of test inocula were significantly predictive of longevity and survival.
D000818:Animals; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D007111:Immunity, Cellular; D007167:Immunotherapy; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008325:Mammary Neoplasms, Experimental / Q000473:pathology / Q000628:therapy*; D009362:Neoplasm Metastasis; D009439:Neuraminidase / Q000378:metabolism; D051381:Rats; D012795:Sialoglycoproteins / Q000276:immunology
D012795:Sialoglycoproteins; D009439:Neuraminidase