Activation of phosphoenolpyruvate-dependent protein kinase by cytidine 5'-triphosphate in rat skeletal muscle.
The effects of nucleotides on phosphoenolpyruvate-dependent protein kinase in rat skeletal muscle were examined. Various ribonucleotide triphosphates were tested and the protein kinase reaction was maximally activated by cytidine 5'-triphosphate followed by uridine 5'-triphosphate and guanosine 5'-triphosphate. No activation by adenosine 5'-triphosphate was observed. Cytidine 5'-diphosphate also activated the reaction but to a significantly lesser extent. Cytidine 5'-monophosphate and cyclic cytidine 3',5'-monophosphate showed no effect whereas cytidine was slightly inhibitory.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D003565:Cytidine Diphosphate / Q000494:pharmacology; D003570:Cytidine Triphosphate / Q000494:pharmacology*; D003597:Cytosine Nucleotides / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D006160:Guanosine Triphosphate / Q000494:pharmacology; D009132:Muscles / Q000201:enzymology*; D009711:Nucleotides / Q000494:pharmacology*; D010728:Phosphoenolpyruvate / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D014544:Uridine Triphosphate / Q000494:pharmacology
D003597:Cytosine Nucleotides; D009711:Nucleotides; D003565:Cytidine Diphosphate; D003570:Cytidine Triphosphate; D010728:Phosphoenolpyruvate; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D014544:Uridine Triphosphate