Reduced acetylcholine sensitivity in dystrophic mouse myotubes in vitro.
Acetylcholine (ACh) sensitivity in cultured myotubes from normal or dystrophic mice was investigated using conventional techniques. Dystrophic multinucleate myotubes were found to be less responsive to the ACh with respect to the control preparation. Such a reduced sensitivity to ACh in dystrophic myotubes was accompanied by a reduced binding to 125I-alpha-bungarotoxin. The possible biological significance of these results on the muscle disease is discussed.
D000109:Acetylcholine / Q000502:physiology*; D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism; D002478:Cells, Cultured; D004553:Electric Conductivity; D066298:In Vitro Techniques; D008564:Membrane Potentials; D051379:Mice; D008810:Mice, Inbred C57BL; D009132:Muscles / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D009137:Muscular Dystrophy, Animal / Q000503:physiopathology*; D011950:Receptors, Cholinergic / Q000502:physiology
D002038:Bungarotoxins; D011950:Receptors, Cholinergic; D000109:Acetylcholine