A modified technique for permeability studies in the superfused hamster cheek pouch.
We report here a modification of the superfused hamster cheek model for use in vascular permeability studies. Radio-iodine labeled serum albumin (I-125 RISA) is injected prior to the superfusion period. Plasma content is calculated on a microliter/100 mg wet weight basis and compared to the contralateral (non-superfused) cheek pouch. Water content is calculated on a percentage basis and compared in the same manner. Results demonstrate that superfusion causes an increase in permeability of protein and water. Plasma content is reduced by catalase, indomethacin or FPL 55712 pretreatment, suggesting that free-radicals, prostaglandins and leukotrienes are released during superfusion. Water content increase is refractory to pretreatment. The advantages of this system and its application are discussed.
D000818:Animals; D002199:Capillary Permeability; D002374:Catalase; D002610:Cheek / Q000098:blood supply; D002867:Chromones; D006224:Cricetinae; D004285:Dogs; D005260:Female; D007213:Indomethacin; D008647:Mesocricetus; D010477:Perfusion / Q000379:methods*
D002867:Chromones; C006312:FPL 55712; D002374:Catalase; D007213:Indomethacin