Determination of plasma oxalate with oxalate oxidase.
A method for the determination of plasma oxalate using oxalate oxidase (EC 1.2.3.4) and deproteinised plasma is described. Reference values are 1.2-6.4 mumol/l (n = 24, mean +/- SD 3.3 +/- 1.5 mumol/l). The sensitivity is 6-7 nmol, the accuracy 3-5 nmol, and the coefficient of variation 10.4% (at a level of 23 nmol). The recovery from plasma spiked with oxalate was 105 +/- 8% (n = 8). Allowing blood to stand for 4 h at room temperature had no effect on plasma oxalate levels; inhibitors of glyoxylate converting enzymes interfered with oxalate oxidase.
D006801:Humans; D006863:Hydrogen-Ion Concentration; D010070:Oxalates / Q000097:blood*; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012016:Reference Values; D013048:Specimen Handling; D013997:Time Factors
D010070:Oxalates; D010088:Oxidoreductases; C020598:oxalate oxidase