Quantitative analysis of phencyclidine and metabolites by capillary column gas chromatography.
A sensitive capillary gas chromatography (GC) procedure was developed for the analysis of phencyclidine (PCP), two of its monohydroxy-metabolites, and a recently identified pentanoic acid metabolite. Two separate, but sequential, integrated extraction techniques were necessary to isolate all of the compounds from a 1.0-mL biological sample. Two GC techniques were necessary to analyze all the compounds; both methods used a capillary column and a nitrogen phosphorus detector (NPD). The first procedure permitted isolation and quantitation of PCP and two monohydroxy-metabolites. A second extraction of the biological samples permitted measurement of the pentanoic acid metabolite. The analytical methods illustrate good reproducibility based on within-day and day-to-day variation. Serum and urine samples were analyzed from a dog administered PCP to illustrate the utility of this procedure.
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D004285:Dogs; D006801:Humans; D006868:Hydrolysis; D006900:Hydroxylation; D008297:Male; D010622:Phencyclidine / Q000032:analysis* / Q000378:metabolism
D010622:Phencyclidine