Effect of denervation on a cholinergic-specific ganglioside antigen (Chol-1) present in Torpedo electromotor presynaptic plasma membranes.
The presence of Chol-1, an antigen identified in the plasma membrane of cholinergic electromotor nerve terminals of Torpedo marmorata, was investigated in Torpedo electric organ after 3, 6, and 9 weeks' denervation. Denervation was monitored by the cessation of stimulus-evoked discharge potentials, by the reduction in nerve terminals seen morphologically, and by the decrease in ACh and ChAT contents. The content of ganglioside-bound sialic acid did not show any appreciable change with time. Some modification of ganglioside pattern on TLC was observed after 9 weeks' denervation. The presence of Chol-1 after denervation was assayed by its activity in inhibiting the selective complement-induced lysis of the cholinergic subpopulation of guinea pig cortical synaptosome which is mediated by the anti-Chol-1 antiserum. Denervation did not affect Chol-1 immunoreactivity although it did alter the distribution of the immunoreactivity among gangliosides. The possible significance of the results is discussed.
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003714:Denervation; D004557:Electric Organ / Q000294:innervation* / Q000648:ultrastructure; D005071:Evoked Potentials; D005732:Gangliosides; D008854:Microscopy, Electron; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000378:metabolism; D013570:Synaptic Membranes / Q000378:metabolism*; D014101:Torpedo
D000954:Antigens, Surface; D005732:Gangliosides; D012794:Sialic Acids; C038777:chol-1 antigen; D002795:Choline O-Acetyltransferase; D019158:N-Acetylneuraminic Acid; D000109:Acetylcholine