Effect of intravenous administration of D-lysergic acid diethylamide on initiation of protein synthesis in a cell-free system derived from brain.
An initiating cell-free protein synthesis system derived from brain was utilized to demonstrate that the intravenous injection of D-lysergic acid diethylamide (LSD) to rabbits resulted in a lesion at the initiation stage of brain protein synthesis. Three inhibitors of initiation, edeine, poly(I), and aurintricarboxylic acid were used to demonstrate a reduction in initiation-dependent amino acid incorporation in the brain cell-free system. One hour after LSD injection, there was also a measurable decrease in the formation of 40S and 80S initiation complexes in vitro, using either [35S]methionine or [35S]Met-tRNAf. Analysis of the methionine pool size after LSD administration indicated there was no change in methionine levels. Analysis of the formation of initiation complexes in the brain cell-free protein synthesis system prepared 6 h after LSD administration indicated that there was a return to control levels at this time. The effects of LSD on steps in the initiation process are thus reversible.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002474:Cell-Free System; D007275:Injections, Intravenous; D008238:Lysergic Acid Diethylamide / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D008715:Methionine / Q000378:metabolism; D010448:Peptide Initiation Factors / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects*; D011817:Rabbits
D010448:Peptide Initiation Factors; D008238:Lysergic Acid Diethylamide; D008715:Methionine