title: [Organotypic culture of normal and neoplastic laryngeal mucosa].
abstract: Mucous membrane's phragments from human normal and neoplastic larynx have been maintained in a organ culture system. A set of cultures has been prepared for optical microscope studies and a parallel set for incorporation studies using 3H Thymidine and 3H Leucine. The results show that there is a good incorporation of 3H Thymidine and a greater incorporation of 3H Leucine at the 7th day due to a differentiation in vitro with a new synthesis of cheratin. These data agree with the morphologic evidence of considerable horny structures in the pathological mucous membrane after 11 days at culture.
mesh_terms: D006801:Humans; D007820:Laryngeal Mucosa / Q000166:cytology* / Q000378:metabolism; D007822:Laryngeal Neoplasms / Q000473:pathology*; D007830:Larynx / Q000166:cytology*; D007930:Leucine / Q000378:metabolism; D009924:Organ Culture Techniques; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
chemical_list: D007930:Leucine; D013936:Thymidine
vernacular_title: Cultura organotipica di mucosa laringea normale e neoplastica.