Specific antibodies to porcine zona pellucida detected by quantitative radioimmunoassay in both fertile and infertile women.
The specific radioimmunoassay system was developed for the titration of the antibodies to porcine zona pellucida (ZP) in human sera by using 125I-labeled purified porcine ZP as antigen, which is known to have cross-reactivity with human ZP. The antibodies in human sera were detected in 3 of 11 (27%) women with unexplained infertility, in 16 of 48 (33%) amenorrheic patients, in 4 of 12 (33%) fertile women, and in 3 of 10 (30%) men. Moreover, antibody titers in infertile women were no higher than those in fertile women and in men. These results seem to suggest that the antibodies in human sera that cross-react with porcine ZP may not be an important factor in causing infertility in women.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000941:Antigens; D003429:Cross Reactions; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000276:immunology*; D010063:Ovum / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D013552:Swine / Q000276:immunology*; D015044:Zona Pellucida / Q000276:immunology*
D000906:Antibodies; D000941:Antigens