title: Effects of stretch and denervation on protease activities of normal and dystrophic chicken muscle.
abstract: Changes of protease activities that follow passive stretch, denervation, and denervation plus stretch were followed in the patagialis muscle of normal and dystrophic chicks between 6 and 7 weeks of age. The baseline activities of cathepsin C, cathepsin D, and leucine aminopeptidase in dystrophic muscle were 2 to 3.5 times higher than in normal muscle. Passive stretch and denervation induced increases in protease activities by 40 to 120% in normal muscle, whereas the same treatments did not significantly affect the activities of the enzymes in dystrophic muscle. We conclude that the level of protease activity in dystrophic chicken muscle at 6 weeks of age had already attained a maximum limit and could not be increased even by denervation. In spite of protease activities, which were not different from control dystrophic muscle, denervated dystrophic muscles lost muscle weight rapidly whether they were stretched or not. They weighed 60% less than the innervated control muscle after 7 days. Inherently high protease activities in dystrophic muscle do not vary at this age regardless of whether or not the muscle is gaining or losing weight.
mesh_terms: D000818:Animals; D002402:Cathepsin D; D002403:Cathepsins / Q000378:metabolism*; D002645:Chickens; D007931:Leucyl Aminopeptidase / Q000378:metabolism*; D009121:Muscle Denervation; D009132:Muscles / Q000201:enzymology* / Q000473:pathology; D009137:Muscular Dystrophy, Animal / Q000201:enzymology* / Q000473:pathology; D009929:Organ Size; D010812:Physical Stimulation; D012016:Reference Values
chemical_list: D002403:Cathepsins; D007931:Leucyl Aminopeptidase; D002402:Cathepsin D