Reversed-phase high-performance liquid chromatography and separation of peptide hormones in human tissues and plasma with reference to cholecystokinins.
A reversed-phase high-performance liquid chromatographic method for the separation of peptide hormones has been applied to the measurement of large and small molecular forms of cholecystokinins in biological tissues. The method was validated for samples of human plasma and intestinal tissues, and for porcine gut extracts. The two-stage chromatographic process used semi-preparative reversed-phase packing for initial sample preparation, followed by gradient elution on a Hypersil ODS-5 column. Peptides in the fmol-nmol range were separated reproducibly, and recovered quantitatively. The method has been applied to the purification of a novel biologically active CCK peptide from a porcine gut extract.
D000818:Animals; D002766:Cholecystokinin / Q000097:blood / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006728:Hormones / Q000097:blood / Q000302:isolation & purification*; D006801:Humans; D007422:Intestines / Q000032:analysis; D010455:Peptides / Q000097:blood / Q000302:isolation & purification*; D011863:Radioimmunoassay / Q000379:methods; D013552:Swine
D006728:Hormones; D010455:Peptides; D002766:Cholecystokinin