Differences in polypeptide composition and enzyme activity between cold-stable and cold-labile microtubules and study of microtubule alkaline phosphatase activity.
Cold-stable and cold-labile microtubules were prepared by two cycles of assembly and disassembly and two periods of exposure to cold. The cold-labile preparations were shown to contain a higher proportion of a high molecular mass microtubule-associated protein (MAP 2) than cold-stable preparations. The cold-stable preparations showed a much higher alkaline phosphatase activity. Stimulation of microtubule assembly by zinc led to increases in both cold stability and alkaline phosphatase activity.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000648:ultrastructure; D003080:Cold Temperature; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008854:Microscopy, Electron; D008869:Microtubule-Associated Proteins; D008870:Microtubules / Q000032:analysis* / Q000187:drug effects; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D051381:Rats; D015032:Zinc / Q000494:pharmacology
D008869:Microtubule-Associated Proteins; D011506:Proteins; D000469:Alkaline Phosphatase; D008274:Magnesium; D015032:Zinc