title: Pancreatic duct glands. II. Lectin binding affinities of ductular epithelium, ductular glands, and Brunner glands.
abstract: Secretory epithelia and ductular glands were studied in main pancreatic ducts of guinea pig, rabbit, rat, and man. Brunner glands were studied for comparison. Semithin sections from Epon-embedded tissues were etched with sodium methylate and incubated with horseradish peroxidase-conjugated lectins. Columnar cells in the epithelium of pancreatic ducts are endowed with well-developed microvillar borders. These apical regions strongly stain with Lotus A-, wheat germ-, and Ricinus I-lectins. Basolateral plasma membranes bind Ricinus I-, Ulex europaeus I-, and wheat germ-lectins. Cytosomes in the supranuclear regions of epithelial cells are interpreted as secretory granules. These droplets are marked by wheat germ-lectin and to a lesser degree by Ricinus I- and Ulex europaeus I-lectins. Ductular glands of the main pancreatic ducts contain secretions that bind Helix-, wheat germ-, and Ulex europaeus I-lectins. Their apical and basolateral cell membranes deeply stain with wheat germ- and Ulex europaeus I-lectins. Secretions of Brunner glands bind Ricinus I-, Ulex europaeus I-, Helix-, and wheat germ lectins. Their apical and basolateral cell membranes stain with Ricinus I- and wheat germ-lectins. - Species differences in lectin-binding affinities of complex carbohydrates were observed and are described.
mesh_terms: D000818:Animals; D002011:Brunner Glands / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis; D004386:Duodenum / Q000032:analysis*; D004848:Epithelium / Q000032:analysis; D005088:Exocrine Glands / Q000032:analysis*; D006168:Guinea Pigs; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins; D010183:Pancreatic Ducts / Q000032:analysis* / Q000648:ultrastructure; D010503:Periodic Acid-Schiff Reaction; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
chemical_list: D002241:Carbohydrates; D037102:Lectins