title: In vitro alterations similar to posttranslational modification of lens proteins.
abstract: Changes similar to posttranslational modifications that are observed with osmotic cataract formation in vivo can be seen with homogenates of lenses. These modifications are the loss of a 31,000 molecular weight (MW) beta crystallin polypeptide, an increase in a 25,000 MW membrane polypeptide. These modifications are potentiated by calcium and occur more rapidly at 37 degrees C than at 4 degrees C. Inhibitors of pepsin or of serine proteases do not influence these modifications, although proteolysis may be responsible for the changes observed.
mesh_terms: D000818:Animals; D002386:Cataract / Q000378:metabolism*; D003459:Crystallins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000378:metabolism*; D008970:Molecular Weight; D010436:Pepstatins / Q000494:pharmacology; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D003459:Crystallins; D010436:Pepstatins; D010664:Phenylmethylsulfonyl Fluoride