Association of sperm receptor activity with a purified pig zona antigen (PPZA).
Three types of data are presented which suggest that sperm receptor activity is associated with a 58,000 Mr acidic glycoprotein (PPZA) isolated from pig zonae: (1) boar sperm fail to bind to pig zonae pretreated with univalent antibody to PPZA; (2) boar sperm pretreated with PPZA are inhibited in binding to intact pig zonae; (3) binding studies utilizing boar sperm and radiolabeled PPZA indicate an inhibition of radiolabeled PPZA binding to sperm by non-labeled PPZA in a dose-related manner. Collectively these data suggest that, at least in the pig system, the contraceptive action of zona antibodies is accomplished by a specific immunologic reaction between antibody and the zona sperm receptor site rather than to a non-specific blockage of this receptor.
D000818:Animals; D000941:Antigens; D001665:Binding Sites; D004527:Egg Proteins; D005260:Female; D005306:Fertilization; D006023:Glycoproteins / Q000276:immunology*; D066298:In Vitro Techniques; D008297:Male; D008562:Membrane Glycoproteins; D010063:Ovum / Q000276:immunology*; D011956:Receptors, Cell Surface; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000276:immunology; D013552:Swine; D015044:Zona Pellucida / Q000276:immunology*; D000072216:Zona Pellucida Glycoproteins
D000941:Antigens; D004527:Egg Proteins; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D000072216:Zona Pellucida Glycoproteins