Age-related structural investigation of the Bronx waltzer mutant mouse cochlea: scanning and transmission electron microscopy.
Cochleas of mice homozygous for the Bronx waltzer gene (symbol bv) were investigated using scanning (SEM) and transmission (TEM) electron microscopy. An age-related study was done from birth to postnatal day 100. With SEM, the arrangement of hair cells confirmed the unique feature of the bv/bv cochlea: the inner hair cells (IHCs) were either absent or abnormally haired but the outer hair cells (OHCs) appeared normal. No significant difference was observed with age. Using TEM, the remaining (20-25%) IHCs could be divided into two groups: normal-looking IHCs but with an abnormal synaptic pole, and abnormal, abortive-like IHCs. Very little if any sign of degeneration was observed whatever the age. OHCs displayed an almost normal cytology and pattern of innervation. The neurons of the spiral ganglion were very rare, even at birth. These findings suggest that the bv mutation should rather be classified in another group, than 'degenerative'. The persistence of normal structures at OHC level is discussed in light of the cochlear physiology of the bv/bv: it again raises the question of the real role of OHCs in the peripheral auditory mechanisms.
D000375:Aging; D000818:Animals; D001306:Auditory Pathways / Q000033:anatomy & histology; D001369:Axons / Q000648:ultrastructure; D003051:Cochlea / Q000033:anatomy & histology*; D003712:Dendrites / Q000648:ultrastructure; D006198:Hair Cells, Auditory / Q000033:anatomy & histology*; D006199:Hair Cells, Auditory, Inner / Q000033:anatomy & histology; D006309:Hearing / Q000502:physiology*; D051379:Mice; D008818:Mice, Neurologic Mutants; D008855:Microscopy, Electron, Scanning; D009925:Organ of Corti / Q000033:anatomy & histology; D013136:Spiral Ganglion / Q000033:anatomy & histology