Comparison of amino acid levels in rat blood obtained by catheterization and decapitation.
A novel, sensitive and highly resolving amino acid analysis procedure was developed and used to compare two methods of obtaining blood from experimental animals. The procedure, utilizing a meter long microbore HPLC column containing spherical cation-exchange resin and fluorescence detection following postcolumn reaction with o-phthaldialdehyde, was shown to reliably measure forty-one primary amine components in rat plasma. Comparison of values from blood obtained by decapitation and by catheterization documented the significant artifactual influence of the decapitation procedure on approximately half of the measured constituents.
D000596:Amino Acids / Q000097:blood*; D000818:Animals; D001800:Blood Specimen Collection / Q000379:methods*; D002404:Catheterization; D002851:Chromatography, High Pressure Liquid; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence
D000596:Amino Acids