High-performance liquid chromatographic analyser for guanidino compounds using benzoin as a fluorogenic reagent.
An automatic analyser based on high-performance liquid chromatography has been developed for the quantification of biogenic guanidino compounds in human physiological fluids. Fourteen guanidino compounds are mutually separated within 35 min on a cation-exchange column with a stepwise gradient elution of pH and/or ionic strength in the mobile phase and then converted automatically to their fluorescent derivatives with benzoin. The method in this system is simple, rapid and sensitive; the lower limits of detection are 5-50 pmol for monosubstituted guanidino compounds, 1 nmol for creatine and 20 nmol for creatinine in 100 microliter of injection volume.
D001573:Benzoin; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006146:Guanidines / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D006878:Hydroxides; D007202:Indicators and Reagents; D007676:Kidney Failure, Chronic / Q000378:metabolism; D011188:Potassium; D017680:Potassium Compounds; D013696:Temperature
D006146:Guanidines; D006878:Hydroxides; D007202:Indicators and Reagents; D017680:Potassium Compounds; D001573:Benzoin; D011188:Potassium; C029943:potassium hydroxide