Isolation of tropoelastin a from lathyritic chick aortae.
Tropoelastin a was isolated from lathyritic chick aortae by using severe denaturing conditions for the initial extraction. The amino acid composition of this new species of tropoelastin is elastin-like in its high proportion of proline, glycine, alanine and valine. However, it differs from authentic tropoelastin b in containing a higher percentage of polar amino acids and cysteine residues. In addition, the amount of proline hydroxylation is 3 times higher than that found in chick tropoelastin b.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001011:Aorta / Q000032:analysis*; D002645:Chickens; D002848:Chromatography, DEAE-Cellulose; D004549:Elastin / Q000031:analogs & derivatives*; D004591:Electrophoresis, Polyacrylamide Gel; D007842:Lathyrism / Q000378:metabolism*; D011489:Protein Denaturation; D014333:Tropoelastin / Q000302:isolation & purification*
D000596:Amino Acids; D014333:Tropoelastin; D004549:Elastin