title: The occurrence of ecdysteroids in the cestode, Moniezia expansa.
abstract: The occurrence of free ecdysteroids in the sheep cestode, Moniezia expansa, was demonstrated. Significant amounts of conjugated ecdysteroids were not detected. Characterization of the free hormones by high-performance liquid chromatography monitoring fractions by radioimmunoassay, and by gas chromatography/mass spectrometry (selected ion monitoring) indicated the presence of ecdysone, 20-hydroxyecdysone and 20,26-dihydroxyecdysone. Analysis of the ecdysteroids by radioimmunoassay in segments along part of the strobila indicated that the anterior parts contained the greatest amount of hormone. GC/MS (SIM) analysis of the hormones in a strobilar segment containing the most mature proglottids suggested the presence of several ecdysteroid metabolites.
mesh_terms: D000818:Animals; D002589:Cestoda / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D026461:Ecdysteroids; D008401:Gas Chromatography-Mass Spectrometry; D007447:Invertebrate Hormones / Q000032:analysis* / Q000302:isolation & purification; D008989:Monieziasis; D011863:Radioimmunoassay
chemical_list: D026461:Ecdysteroids; D007447:Invertebrate Hormones