The temperature dependence of the fluorescence decay of low-density lipoproteins.
The decay of the intrinsic tryptophan fluorescence of low-density lipoproteins (LDL) is measured using a picosecond laser system with an excitation wavelength of 295 nm. The emission wavelengths were observed at 320, 340 and 360 nm. The measurements were performed in the temperature range 10-45 degrees C. The fluorescence decay data are analysed in terms of: (i) three exponentials; (ii) a continuous distribution of exponentials; and (iii) the average decay time. The results indicate that the tryptophans are in a non-polar environment and that the fluorescence is not affected by phase transitions found to occur in the lipid core of the LDL molecule.
D000817:Anilino Naphthalenesulfonates; D055598:Chemical Phenomena; D002621:Chemistry; D006801:Humans; D008077:Lipoproteins, LDL; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013997:Time Factors; D014364:Tryptophan
D000817:Anilino Naphthalenesulfonates; D008077:Lipoproteins, LDL; C027132:1-anilino-8-naphthalenesulfonate; D014364:Tryptophan