Nature of the binding site around and reactivity of histidine-15 in lysozyme.
Bromoacetamide derivatives having n-alkyl substituents (BrCH2CONH(CH2)nH, 1-n) and carboxyalkyl substituents (BrCH2CONH(CH2)nCOOH, 2-n) react with His-15 in lysozyme exclusively at N epsilon 2 at pH 5.5 and 40 degree C. Kinetic studies suggest that lysozyme has a small hydrophobic pocket that binds these reagents in the vicinity of His-15.
D000478:Alkylation; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens; D002852:Chromatography, Ion Exchange; D006639:Histidine / Q000378:metabolism*; D007700:Kinetics; D009113:Muramidase / Q000378:metabolism*
D000596:Amino Acids; D006639:Histidine; D009113:Muramidase