title: Determination of lidocaine and active metabolites in blood serum by liquid chromatography with electrochemical detection.
abstract: Oxidation of lidocaine and its principal metabolites, monoethylglycine xylidide and glycine xylidide, at glassy carbon electrodes was employed to permit electrochemical detection of these compounds following separation by high-performance liquid chromatography. The absolute detection limits found for these compounds were 2 ng, 5 ng, and 4 ng injected, respectively. The resulting assay was suitable for the routine quantitation of lidocaine and these metabolites in blood serum over the entire therapeutic range, 1-6 micrograms/ml. Total analysis time is 10-15 min.
mesh_terms: D002045:Bupivacaine / Q000097:blood; D002853:Chromatography, Liquid / Q000379:methods; D004563:Electrochemistry; D006801:Humans; D008012:Lidocaine / Q000031:analogs & derivatives / Q000097:blood*; D013997:Time Factors
chemical_list: D008012:Lidocaine; C001306:glycinexylidide; C001173:monoethylglycinexylidide; D002045:Bupivacaine