Comparison of protein-synthesis rate of alveolar macrophages in vivo and in vitro.
This paper describes and validates a novel method for measuring rates of protein synthesis of rabbit alveolar macrophages in vivo. A rate of 9.3%/day was obtained, compared with 48.9%/day measured in vitro. This study suggests that the procedures involved in the isolation of alveolar macrophages for study in vitro may themselves activate the cell.
D000818:Animals; D002478:Cells, Cultured; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010649:Phenylalanine / Q000378:metabolism; D011392:Proline / Q000378:metabolism / Q000494:pharmacology; D014176:Protein Biosynthesis; D011650:Pulmonary Alveoli / Q000166:cytology*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013997:Time Factors
D012710:Serum Albumin, Bovine; D010649:Phenylalanine; D011392:Proline