Subcellular aldehyde dehydrogenase activity and acetaldehyde oxidation by isolated intact mitochondria of rat brain and liver after acetaldehyde treatment.
The effect of treatment of rats with acetaldehyde on the subcellular NAD+-aldehyde dehydrogenase (EC 1.2.1.3, ALDH) activities and acetaldehyde oxidation by isolated intact mitochondria of the liver and the brain was studied. Inhalation of acetaldehyde caused a significant decrease in the liver mitochondrial low Km-ALDH activity, while brain mitochondrial ALDH activity remained unchanged. Acetaldehyde oxidation by isolated intact liver mitochondria decreased significantly but that by brain mitochondria remained unchanged after acetaldehyde inhalation. These findings raise the possibility that the brain enzyme may be exposed to lower concentration of acetaldehyde than the liver enzyme.
D000079:Acetaldehyde / Q000097:blood / Q000378:metabolism / Q000633:toxicity*; D000444:Aldehyde Dehydrogenase; D000445:Aldehyde Oxidoreductases / Q000037:antagonists & inhibitors*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology*; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology
D000445:Aldehyde Oxidoreductases; D000444:Aldehyde Dehydrogenase; D000079:Acetaldehyde