Rat liver histidyl-tRNA synthetase. Purification and inhibition by the myositis-specific anti-Jo-1 autoantibody.
Myositis is an autoimmune inflammatory muscle disease of unknown etiology. We demonstrate directly that the antigen to the myositis-specific anti-Jo-1 antibody is histidyl-tRNA synthetase. The anti-Jo-1 antibody inhibits human HeLa and rat liver histidyl-tRNA synthetase. Using conventional and immunoaffinity chromatography with immobilized anti-Jo-1 antibody, we have purified rat liver histidyl-tRNA synthetase which has a subunit Mr 64,000 and an estimated native Mr suggesting an alpha 2 structure. The evidence indicates that the Jo-1 antigen is histidyl-tRNA synthetase, and that some of the histidyl-tRNA synthetase structure are conserved across species.
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D000818:Animals; D001323:Autoantibodies / Q000494:pharmacology*; D002846:Chromatography, Affinity; D006367:HeLa Cells; D006643:Histidine-tRNA Ligase / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D006801:Humans; D007120:Immunochemistry; D008099:Liver / Q000201:enzymology*; D009220:Myositis / Q000276:immunology*; D051381:Rats
D001323:Autoantibodies; D000604:Amino Acyl-tRNA Synthetases; D006643:Histidine-tRNA Ligase