title: Mutant hemoglobin stability depends upon location and nature of single point mutation.
abstract: The temperature dependence of the rates of heme release from the beta subunits of methemoglobin A and 5 beta mutant methemoglobins has been determined. The rates were largest for two hemoglobins with mutations distal to heme, previously known to be unstable. The other 3 mutants also released heme faster than A. These hemoglobins, with single point mutations at the alpha 1/beta 2 interface, were previously thought to be stable. The low reported yields of the 5 mutant proteins covaries with the relative rates of heme release from the met species.
mesh_terms: D006418:Heme / Q000378:metabolism; D006441:Hemoglobin A / Q000378:metabolism*; D006455:Hemoglobins, Abnormal / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008706:Methemoglobin / Q000378:metabolism*; D013696:Temperature
chemical_list: D006455:Hemoglobins, Abnormal; C011478:hemoglobin Hammersmith; D006418:Heme; C028905:hemoglobin Hotel-Dieu; C030556:hemoglobin Saint Mande; D008706:Methemoglobin; D006441:Hemoglobin A; C034409:hemoglobin Koln; C041172:hemoglobin Malmo