A simple and rapid enzymatic determination of L-phenylalanine with a novel L-phenylalanine oxidase (deaminating and decarboxylating) from Pseudomonas sp. P-501.
A simple and rapid method for the determination of L-phenylalanine using Pseudomonas L-phenylalanine oxidase (deaminating and decarboxylating) has been developed. This method involves the oxidative deamination of L-phenylalanine using L-phenylalanine oxidase from Pseudomonas sp. P-501, and the colorimetric determination of hydrogen peroxide formed from L-phenylalanine with 4-aminoantipyrine and N,N'-dimethylaniline in the presence of peroxidase. This method requires a smaller quantity of sample and is less time-consuming than those previously reported. The method can be used for the direct assay of serum L-phenylalanine levels without pretreatment.
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D001322:Autoanalysis; D006801:Humans; D010649:Phenylalanine / Q000032:analysis* / Q000097:blood; D011549:Pseudomonas / Q000201:enzymology*; D013053:Spectrophotometry
D010649:Phenylalanine; D000594:Amino Acid Oxidoreductases; C022342:phenylalanine oxidase