Kidney urokinase activity following acute exposure to lead.
Urokinase activity was measured in kidney homogenate fractions obtained 2 days after injecting rats with 0.5 mg Pb2+/100 g body weight. The activity was higher in the membrane-containing fractions than in the soluble supernatant fractions and was markedly higher in the preparations derived from the lead-treated rats. The kidney poly(A)+ RNA was obtained from these animals and translated in a rabbit reticulocyte lysate system. In the preparations obtained from the lead-treated rats, there was an increased synthesis of a protein, believed to be urokinase, of pl 8.6 and molecular weight (Mr) 45,000.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D007525:Isoelectric Focusing; D007668:Kidney / Q000201:enzymology*; D007855:Lead Poisoning / Q000201:enzymology*; D008297:Male; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism*
D012333:RNA, Messenger; D014568:Urokinase-Type Plasminogen Activator