title: Characterization of the estrogen receptor extracted from hen oviduct nuclei with pyridoxal phosphate.
abstract: The estrogen receptor was extracted in high yield from nuclei of laying hen oviduct with 10 mM pyridoxal-5'-phosphate (PLP). The receptor extracted under these conditions, unlike that extracted with 0.4 M KCl, displayed no tendency to aggregate on sucrose gradients in low salt. The receptor was eluted as a single peak from DEAE-Sephacel at an ionic strength of 0.13 M KCl. The receptor after DEAE chromatography had approximately half the molecular weight of that in the nuclear extract. A larger form could be reconstituted by the addition of whole nuclear extract to the DEAE eluate. These data support the notion that the nuclear estrogen receptor is a dimer composed of similar subunits.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002645:Chickens; D002852:Chromatography, Ion Exchange; D005260:Female; D010057:Oviducts / Q000032:analysis*; D011732:Pyridoxal Phosphate / Q000494:pharmacology*; D011960:Receptors, Estrogen / Q000302:isolation & purification*
chemical_list: D011960:Receptors, Estrogen; D011732:Pyridoxal Phosphate