title: KLE: a cell line with defective estrogen receptor derived from undifferentiated endometrial cancer.
abstract: KLE is a cell line derived from a poorly differentiated endometrial carcinoma that is aneuploid with chromosome numbers ranging from 51 to 66 and 6-8 marker chromosomes demonstrated by G banding. Tumors harvested from five of five nude mice bearing an inoculum for more than a month resemble the original specimen, and electron microscopy shows microvilli, many junctional processes, glycogenation, and a prominent nucleolonema. The cell cytosol contains a specific binder for estradiol, but there is no estrogen receptor in the nucleus and in a study reported elsewhere (Raam et al., Breast Cancer Res. Treat. 2, 277 (1982) ) translocation to the nucleus fails to occur. The enzyme phenotype of this cell is human, non-HeLa.
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D000368:Aged; D000782:Aneuploidy; D000818:Animals; D002460:Cell Line; D005260:Female; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D010641:Phenotype; D011960:Receptors, Estrogen / Q000032:analysis*; D014183:Transplantation, Heterologous; D014594:Uterine Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure
chemical_list: D011960:Receptors, Estrogen