Quantitative analysis of emetine and cephaeline by reversed-phase high performance liquid chromatography with fluorescence detection.
A versatile method for the quantitation of emetine and cephaeline in biological samples is described. Two milliliters of samples containing N-propylprocainamide as the internal standard are buffered to pH 9 and extracted with n-butyl chloride. After subsequent back extraction into 0.01 M hydrochloric acid, a portion of the acid layer is analyzed by reversed-phase high performance liquid chromatography with fluorescence detection. Routinely, the minimum level of detection for both drugs is 5 ng/mL and linearity is demonstrated from 5 to 2500 ng/mL.
D001826:Body Fluids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004640:Emetine / Q000031:analogs & derivatives* / Q000032:analysis*; D006801:Humans; D013050:Spectrometry, Fluorescence / Q000379:methods
C005963:cephaelin; D004640:Emetine