The effect of interferon on cells deficient in nucleoside transport or lacking thymidine kinase activity.
Mutants of mouse T-lymphoma S49 cells lacking thymidine kinase activity or deficient in nucleoside transport were selected by growth in the presence of 5'-fluorodeoxyuridine and their sensitivity to interferon tested. All five thymidine kinase and both transport deficient mutants were sensitive to the antiproliferative effects of interferon. The replication of encephalomyocarditis virus was also inhibited by interferon, and the intracellular levels of (2'-5')oligo-adenylate synthetase were elevated in all mutants tested. These results suggest that an intact nucleoside transport system or thymidine kinase activity are not essential for the expression of interferon sensitivity in these cells.
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells; D004790:Enzyme Induction; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007700:Kinetics; D008223:Lymphoma / Q000378:metabolism*; D051379:Mice; D009705:Nucleosides / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism*; D013937:Thymidine Kinase / Q000172:deficiency* / Q000378:metabolism
D007370:Interferon Type I; D009705:Nucleosides; D013937:Thymidine Kinase; D015088:2',5'-Oligoadenylate Synthetase; D013936:Thymidine