Dietary trilinoelaidate: effects on organ fatty acid composition, prostanoid biosynthesis and platelet function in rats.
Incremental levels of trilinoelaidate (tt18:2) were fed to rats for 11 weeks and changes in lung and epididymal lipid fatty acid composition were determined, and concentrations of prostanoids in serum, lung and stomach fundus were measured. Platelet aggregation to various agonists was tested. In the lung there was a concomitant increase of linoelaidate corresponding to incremental dietary levels in all lipid classes (phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine-phosphatidylinositol and neutral lipids). Arachidonic acid in lung phosphatidylcholine was markedly decreased. Epididymis accumulated linoelaidate in only the neutral lipid fraction; no tt18:2 was found in any of the phospholipid classes. Serum prostanoid levels (thromboxane B2 and prostaglandins PGF2 alpha and PGE) were significantly decreased in rats fed high levels of tt18:2, but were not altered at lower levels of consumption; the concentrations of 6-keto-PGF1 alpha were not significantly altered at any level of tt18:2. Platelet responsiveness to various agonists was not significantly altered by incremental dietary trilinoelaidate, i.e., ADP, thrombin, collagen and calcium elicited similar aggregation responses. In conclusion, dietary trilinoelaidate at low levels of consumption, while being incorporated into various lipid classes, did not alter serum and tissue prostanoid levels or markedly affect platelet aggregation.
D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D004041:Dietary Fats / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004822:Epididymis / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005748:Gastric Fundus / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects; D011453:Prostaglandins / Q000096:biosynthesis*; D051381:Rats; D014280:Triglycerides / Q000008:administration & dosage* / Q000378:metabolism
D004041:Dietary Fats; D005227:Fatty Acids; D011453:Prostaglandins; D014280:Triglycerides; C009564:trilinolein