Preparation and one-step purification of mono-125I-angiotensin II for radioligand binding assays.
A one-step purification of mono-125I-angiotensin II prepared by the chloramine T procedure is described. The purification is effected on a cellulose cation exchange column with isocratic elution by 50 mM sodium acetate, pH 5.0. The purity of the mono-125I-angiotensin II was determined by thin layer chromatography, high pressure liquid chromatography, enzymatic digestion, radioreceptor assay, and radioimmunoassay. Preparation and purification of mono-125I-angiotensin II by this procedure offers significant advantages over existing methods for its preparation in terms of purity, simplicity, efficiency, and cost.
D000311:Adrenal Glands / Q000032:analysis; D000804:Angiotensin II / Q000138:chemical synthesis* / Q000302:isolation & purification; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D004285:Dogs; D007457:Iodine Radioisotopes; D008722:Methods; D011869:Radioligand Assay; D051381:Rats; D011945:Receptors, Angiotensin / Q000032:analysis
D007457:Iodine Radioisotopes; D011945:Receptors, Angiotensin; D000804:Angiotensin II