Ontogeny of ppp(A2'p)nA-binding protein in mouse brain.
Mouse brain tissue extracts at various stages of development show a drastic change in the specific activity of pp(A2'p)3A-[32P]pCp binding protein. Identification of the ppp(A2'p)3A-[32P]pCp binding protein was established by (i) binding to the specific ligand ppp(A2'p)3A-[32P]pCp, (ii) displacement of binding by nanomolar concentrations of pppA(pA)3, and (iii) affinity labeling techniques in which periodate oxidized ppp(A2'p)3A-[32P]pC was specifically cross-linked to a protein with a molecular weight of 85000. These data suggest that the ppp(A2'p)3A-[32P]pCp protein is closely associated with the process of cellular proliferation and differentiation.
D000375:Aging; D000818:Animals; D001921:Brain / Q000254:growth & development* / Q000378:metabolism; D004722:Endoribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D051379:Mice; D008970:Molecular Weight; D010761:Phosphorus Radioisotopes; D011247:Pregnancy; D012260:Ribonucleases / Q000302:isolation & purification / Q000378:metabolism*
D010761:Phosphorus Radioisotopes; D004722:Endoribonucleases; D012260:Ribonucleases; C031956:2-5A-dependent ribonuclease; C034663:ppp(A2'p)nA-dependent endoribonuclease protein, mouse