Nature of products formed in the vitamin K-dependent carboxylation of synthetic peptides.
Vitamin K-dependent carboxylation of synthetic Phe-Leu-Glu-Glu-Val by rat liver microsomes yields a secondary product, which has been identified as Leu-Gla-Glu-Val. Similar results are obtained with other synthetic substrates. The microsomal preparation has been shown to contain an aminopeptidase activity which splits carboxylation products and substrates but is unable to hydrolyse the Leu-Gla peptide bond.
D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism*; D051381:Rats; D013552:Swine; D014812:Vitamin K / Q000494:pharmacology*; D014813:Vitamin K Deficiency / Q000378:metabolism*
D002250:Carbon Radioisotopes; D009842:Oligopeptides; D014812:Vitamin K; D000626:Aminopeptidases; D007931:Leucyl Aminopeptidase