Induction of autoimmune damage to spermatogenesis in guinea pigs by passive transfer of anti-F9 mouse teratocarcinoma stem cell serum fraction.
The anti-F9 and anti-spermatogenic cell sera with known cytotoxic and immunofluorescent activities were produced in albino guinea pigs immunized with F9 or spermatogenic cells emulsified with Freund's complete adjuvant. Short-term administration of the globulin fractions (3 x 50 mg at 24-h intervals + 1 x FCA on the first day) obtained from these antisera to adult albino guinea pigs resulted in a significant inhibition of spermatogenesis and appearance in the sera of marked cytotoxic activity against F9 cells. These results together with the impairment of fertility in male and female mice immunized with teratocarcinoma stem cells described by other authors are interpreted as supporting the hypothesis that the F9 antigens may play an essential role in spermatogenesis and function of spermatozoa.
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D001323:Autoantibodies; D002454:Cell Differentiation; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D006168:Guinea Pigs; D007116:Immunization, Passive; D008297:Male; D051379:Mice; D013091:Spermatogenesis; D013724:Teratoma / Q000276:immunology*
D000951:Antigens, Neoplasm; D001323:Autoantibodies; C031192:oncofetal antigens