The concentration of free calcium ions in capillary blood from neonates on a routine basis using the ICA 1.
The concentration of free calcium ions (cCa2+), and pH was measured in 200 full-term infants aged 5-130 h and in 50 healthy adults aged 18-60 years with a new semi-automatic electrode system (ICA 1 Radiometer, Copenhagen) in 110 microliter capillary whole blood. The blood was obtained anaerobically by heel puncture of the infants and from the earlobe of the adults. No bias was found for the erythrocyte effect on the liquid junction potential with a haematocrit below 0.60 but for a haematocrit between 0.60 and 0.80 there was an increasing negative bias of 1-5% for this instrument. The special heparinized glass capillaries (Radiometer, Copenhagen) used for blood sampling were studied in serum pools at different levels of cCa2+ and the anticoagulant used was shown to give a bias of less than +/- 1% on the measurement of cCa2+ in the range 0.86-2.02 mmol/l. In full-term infants aged 5-48 h cCa2+ was 1.27 +/- 0.098 mmol/l (+/- 2 SD) and pH 7.42 +/- 0.07 (+/- 2 SD). In infants aged 49-96 h, cCa2+ was 1.334 +/- 0.136 mmol/l (+/- 2 SD) and pH 7.42 +/- 0.07 (+/- 2 SD). In infants, aged 97-130 h cCa2+ was 1.383 +/- 0.140 mmol/l (+/- 2 SD) and pH 7.40 +/- 0.076 (+/- 2 SD). In capillary blood from healthy adults, aged 18-60 years cCa2+ was 1.28 +/- 0.07 mmol/l (+/- 2 SD) and pH 7.42 +/- 0.03 (+/- 2 SD).(ABSTRACT TRUNCATED AT 250 WORDS)
D000293:Adolescent; D000328:Adult; D001800:Blood Specimen Collection; D002118:Calcium / Q000097:blood*; D002196:Capillaries; D004566:Electrodes; D005260:Female; D006400:Hematocrit; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D011786:Quality Control; D011874:Radiometry / Q000295:instrumentation / Q000592:standards; D012016:Reference Values
D006493:Heparin; D002118:Calcium