title: [Cold-adapted strain of A/Leningrad/134/47/57 (H2N2)--a special attenuation donor of live influenza vaccine for children and the isolation of its recombinants].
abstract: A cold-adapted A/Leningrad/134/47/57 (H2N2) strain, a special donor of attenuation for generation of recombinant vaccine strains safe for children, has been developed and characterized. The genetic markers of this virus (temperature sensitivity of the cold-adapted strain, temperature optimum of the neuraminidase activity) were compared with those of A/Leningrad/134/17/57 (H2N2) strain, a donor of attenuation for a live influenza vaccine for adults. The A/Leningrad/134/47/57 (H2N2) strain was found to have a more marked temperature sensitivity and cold adaptation which correlated with the presence in its genome of multiple temperature-sensitive mutations in genes 1, 2, 5, 7, and 8. When the cold-adapted A/Leningrad/134/47/57 (H2N2) strain was hybridized with epidemic A/Brazil/11/78 (H1N1) and A/Bangkok/1/79 (H3N2) strains, recombinants were isolated which are candidate vaccine strains for a live influenza vaccine for children. The recombinants inherited the antigenic specificity of the current viruses and the temperature specificity and cold-adaptation of the donor of attenuation, A/Leningrad/134/47/57 (H2N2).
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002648:Child; D003080:Cold Temperature; D006801:Humans; D053121:Influenza A Virus, H2N2 Subtype; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D007252:Influenza Vaccines / Q000276:immunology / Q000302:isolation & purification*; D051379:Mice; D011995:Recombination, Genetic; D013696:Temperature; D014613:Vaccines, Attenuated / Q000276:immunology / Q000302:isolation & purification; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D007252:Influenza Vaccines; D014613:Vaccines, Attenuated
vernacular_title: Kholodoadaptirovannyĭ shtamm A/Leningrad/134/47/57 (H2N2)--spetsialnyĭ donor attenuatsii zhivoĭ grikpoznoĭ vaktsiny dlia deteĭ i poluchenie na ego osnove rekombinantov.