title: A two-active site one-polypeptide enzyme: the isomaltase from sea lion small intestinal brush-border membrane. Its possible phylogenetic relationship with sucrase-isomaltase.
abstract: The enzyme responsible for all of the isomaltase activity and much of the maltase activity in the small intestine of the Californian sea lion (Zalophus californianus) was isolated by detergent solubilization of the brush-border membrane, followed by immunoadsorption chromatography using antibodies directed against rabbit sucrase-isomaltase. In 0.1% Triton X-100, sea lion isomaltase occurs as a monomer of Mr = 245,000 and is composed of a single polypeptide chain. As judged from the stoichiometry of the covalent binding of the affinity label, conduritol-B-epoxide, this polypeptide chain carries two enzymatically active sites; they are apparently identical and do not show either positive or negative cooperativity. In addition to cross-reacting immunologically with rabbit sucrase-isomaltase, sea lion isomaltase has similar overall enzymatic properties, with the exception of not hydrolyzing sucrose. The Alaskan fur seal (Collarhinus ursinus) has a two-active site isomaltase; however, in contrast to the sea lion, this animal is endowed with a small but significant sucrase activity. Along with (fully active) pro-sucrase-isomaltase, sea lion isomaltase is one of the very few examples of enzymes with more than one active site on a single polypeptide chain acting "in parallel" (rather than "in series"). Furthermore, this enzyme triggers some interesting questions on the phylogenetical pedigree of small intestinal sucrase-isomaltase.
mesh_terms: D000818:Animals; D001665:Binding Sites; D010872:Caniformia / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007421:Intestine, Small / Q000201:enzymology*; D007700:Kinetics; D008871:Microvilli / Q000201:enzymology*; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000235:genetics*; D009834:Oligo-1,6-Glucosidase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010802:Phylogeny; D011817:Rabbits; D046070:Sea Lions / Q000378:metabolism*; D013045:Species Specificity; D013394:Sucrase-Isomaltase Complex / Q000235:genetics*; D013552:Swine
chemical_list: D009097:Multienzyme Complexes; D006026:Glycoside Hydrolases; D013394:Sucrase-Isomaltase Complex; D009834:Oligo-1,6-Glucosidase