Immunological similarity of milk sulfhydryl oxidase and kidney glutathione oxidase.
A radioimmunoassay for sulfhydryl oxidase, a membrane enzyme, was developed using antibodies raised to the bovine milk enzyme which had been purified by transient covalent affinity chromatography on a cysteinylsuccinamidopropyl-glass matrix. Bovine milk sulfhydryl oxidase and bovine kidney sulfhydryl oxidase ("glutathione oxidase") appear to be immunologically identical as evidenced by parallel responses in radioimmunoassays. Antibodies raised to the purified milk sulfhydryl oxidase can immunoprecipitate glutathione oxidase activity, but not gamma-glutamyltransferase ("transpeptidase") activity, from bovine kidney preparations.
D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D005260:Female; D007668:Kidney / Q000201:enzymology*; D008892:Milk / Q000201:enzymology*; D010088:Oxidoreductases / Q000276:immunology*; D011863:Radioimmunoassay; D050862:Oxidoreductases Acting on Sulfur Group Donors
D010088:Oxidoreductases; D050862:Oxidoreductases Acting on Sulfur Group Donors; C020667:glutathione oxidase