Is cytoplasmic Ca2+ in lymphocytes elevated in cystic fibrosis?
An increased cytoplasmic Ca2+ concentration ( [Ca2+]i) has been implicated in the pathogenesis of cystic fibrosis. We compared the [Ca2+]i levels of normal and cystic fibrosis peripheral blood lymphocytes and Epstein-Barr virus-transformed lymphoblasts using quin 2, an internally trapped indicator. The [Ca2+]i levels of normal and cystic fibrosis cells were not significantly different. The ionophore-releasable intracellular Ca2+ stores were also comparable in both types of individual.
D000293:Adolescent; D000328:Adult; D000634:Aminoquinolines; D002118:Calcium / Q000378:metabolism*; D002451:Cell Compartmentation; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000378:metabolism*; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D007476:Ionophores; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
D000634:Aminoquinolines; D005456:Fluorescent Dyes; D007476:Ionophores; D002118:Calcium