Kinetics of cold-induced denaturation of metmyoglobin.
Using a slow temperature-jump spectrophotometer, we have studied the kinetics of cold-induced denaturation of metmyoglobin between 0 degrees C and 20 degrees C at acidic pH. The time-scale of the transition is slow and is of the order of minutes. The results are consistent with the transition's involving a total of three states, native (N), transient intermediate (I) and denatured (D), which are converted from one to the other in that order.
D000818:Animals; D003080:Cold Temperature; D006420:Hemeproteins; D006736:Horses; D007700:Kinetics; D008786:Metmyoglobin; D011489:Protein Denaturation; D013057:Spectrum Analysis
D006420:Hemeproteins; D008786:Metmyoglobin