Characteristics of pancreatic branched chain amino acid aminotransferases from rat, man and dog.
Branched chain amino acid aminotransferase (EC 2.6.1.42) from pancreas of rat, man and dog was highly purified and characterized. Rat pancreas contained only one type of branched chain amino acid aminotransferase, but human and dog pancreas contained two types. These were named Enzyme I and Enzyme III according to the classification by Aki et al. (1969). Five enzymes purified were activated by the addition of 2-mercaptoethanol and glutathione. Enzyme III has lower Km values for amino acids and 2-oxo acids than Enzyme I. Some other properties of purified enzymes are described.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004285:Dogs; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007700:Kinetics; D008970:Molecular Weight; D010179:Pancreas / Q000201:enzymology*; D011487:Protein Conformation; D051381:Rats; D013045:Species Specificity; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000032:analysis; D000637:Transaminases / Q000032:analysis*
D013438:Sulfhydryl Compounds; D000637:Transaminases; C019798:branched-chain-amino-acid transaminase