Molecular clones of the mouse t complex derived from microdissected metaphase chromosomes.
Fragments of the proximal half of mouse chromosome 17 including the t-complex region were microdissected from metaphase spreads. DNA was isolated from a pool of such fragments, and was cloned on microscale. Individual clones were used to probe genomic digests of DNA from a pair of Chinese hamster cell lines with or without mouse chromosome 17, and livers of congenic inbred lines of mice carrying wild-type and/or t-haplotype forms of chromosome 17. The data obtained indicate that 95% of the low copy number microclone inserts recognize DNA sequences present on mouse chromosome 17. It has been possible to use one-third of these clones to identify restriction-fragment-length polymorphisms between wild-type and t-haplotype DNA on a congenic background. These results demonstrate that these clones have been derived from the t-complex or regions closely linked to it. Clones of this type should provide starting points for a molecular analysis of this region of the mouse genome.
D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure; D003001:Cloning, Molecular / Q000379:methods*; D008677:Metaphase; D051379:Mice; D008817:Mice, Mutant Strains / Q000235:genetics*