title: Impairment of acetylcholine synthesis in thiamine deficient rats developed by prolonged tea consumption.
abstract: The synthesis of whole brain acetylcholine is reduced in thiamine deficient rats produced by prolonged administration of tea. In those rats fed a normal diet and given tea (1:50, w/v) instead of drinking water for 20 weeks, the conversion of [14C] pyruvate to [14C]acetylcholine decreased by 35%. However, no neurological symptoms were observed. Administration of tea to rats fed a thiamine half-deficient diet for 7-8 weeks caused not only 60% decrease in acetylcholine synthesis but also neurological symptoms. This decreased synthesis of acetylcholine is related to a decline in pyruvate dehydrogenase activity. The results suggest that prolonged administration of tea to rats cause an impairment of acetyl CoA production resulting in a deficit in acetylcholine synthesizing capacity.
mesh_terms: D000109:Acetylcholine / Q000096:biosynthesis*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism; D051381:Rats; D013662:Tea; D013832:Thiamine Deficiency / Q000209:etiology / Q000378:metabolism*
chemical_list: D011768:Pyruvate Dehydrogenase Complex; D013662:Tea; D000109:Acetylcholine