Nasal polyps. Scanning electron microscopy and artifact.
Nasal polyps are grossly oedematous tissue which has undergone considerable shrinkage when processed for scanning electron microscopy (SEM), converting the normally smooth surface into one with undulations. This shrinkage resulted in variable degrees of loss of the surface epithelium and thus gave rise to artifact. In its severest form, the entire surface epithelium was removed and the basement membrane exposed; lesser damage resulted in exposure of the basal cells, giving the previously described 'cobblestone' appearance. When the epithelium was intact, it was typical respiratory in type.
D000328:Adult; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004848:Epithelium / Q000648:ultrastructure; D006801:Humans; D008407:Mast Cells / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009298:Nasal Polyps / Q000648:ultrastructure*; D014420:Turbinates / Q000648:ultrastructure