Orientation of nucleosomes and linker DNA in calf thymus chromatin determined by photochemical dichroism.
The dichroism for photochemical attachment of a psoralen derivative to Mg2+-stabilized chromatin fibres is used to deduce the orientation of nucleosomal disks and linker DNA in the 30-nm fibre. The new technique of photochemical electric dichroism should have general applicability to problems of nucleic acid organization in cellular subunits and viruses.
D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000648:ultrastructure*; D013950:Thymus Gland; D014307:Trioxsalen / Q000031:analogs & derivatives
D002843:Chromatin; D009707:Nucleosomes; D004247:DNA; C018822:aminomethyltrioxsalen; D014307:Trioxsalen