title: Amino-acid sequence and disulfide linkages of the anaphylatoxin, des-Arg omega-C5a, from porcine serum.
abstract: The primary structure of the porcine complement-derived peptide, des-Arg omega-C5a, has been analysed. Des-Arg omega-C5a is the natural secondary product of the activation fragment of the fifth component of complement, C5a, and represents a classical anaphylatoxin. The elaborated amino-acid sequence confirms the structure of porcine C5a proposed earlier by Gerard and Hugli, with one exception. Further, by end-group determination and sequencing of the unreduced core of des-Arg omega-C5a the position of its three disulfide bridges has been determined, now allowing insight into the tertiary structure of des-Arg omega-C5a.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003182:Complement C5 / Q000031:analogs & derivatives*; D015937:Complement C5a, des-Arginine; D003545:Cysteine / Q000032:analysis; D003553:Cystine / Q000032:analysis; D004220:Disulfides / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D013552:Swine
chemical_list: D003182:Complement C5; D015937:Complement C5a, des-Arginine; D004220:Disulfides; D003553:Cystine; D003545:Cysteine