Glutathione and GSH-dependent enzymes in the human gastric mucosa.
The gamma-glutamyl-transferase activity, the total glutathione content, the GSH-peroxidase activity, and the GSH S-transferase activity using an aryl substrate were estimated in the S9 fraction of gastric biopsy specimens taken from patients with normal stomach morphology (n = 24), acute gastritis (n = 15), chronic-atrophic gastritis (n = 10), gastric ulcer (n = 9), and carcinoma of the stomach (n = 12). The total glutathione content of normal gastric mucosal specimens was significantly higher than that of human liver biopsy specimens, whereas the GSH-peroxidase and the GSH S-aryltransferase activities were much lower than those found in the liver. Specimens of gastric ulcer had significantly lower enzyme activities of GSH-peroxidase and GSH-aryltransferase, whereas gastric cancer tissue had significantly lower concentrations of total glutathione. The intraindividual comparison of tumorous and non-tumorous tissue showed a consistent decrease of total glutathione as well as of GSH-aryltransferase activity in carcinomatous tissue.
D000230:Adenocarcinoma / Q000201:enzymology; D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000201:enzymology; D005260:Female; D005753:Gastric Mucosa / Q000201:enzymology*; D005756:Gastritis / Q000201:enzymology; D005757:Gastritis, Atrophic / Q000201:enzymology; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D013272:Stomach Diseases / Q000201:enzymology*; D013274:Stomach Neoplasms / Q000201:enzymology; D013276:Stomach Ulcer / Q000201:enzymology
D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D005978:Glutathione