Mechanisms of decreased leucocyte adhesiveness and migration in plasma from patients with IgG myelomatosis.
The adhesiveness of leucocytes in human plasma was decreased by the addition of IgG M-protein, commercial IgG, F(ab)2-fragments, or bovine serum albumin (BSA). Leucocyte migration was inhibited by IgG M-protein and commercial IgG, but not by reduced and alkylated IgG, F(ab)2-fragments or BSA. Guinea pig serum (GPS) strongly enhanced leucocyte migration in Hanks' balanced salt solution. This effect was abolished by hydrazine treatment or heating, and reduced, but not abolished, by zymosan treatment. C4-deficient guinea pig serum (C4-def-GPS), but not zymosan treated C4-def-GPS stimulated migration. In IgG myeloma sera migration was enhanced by the addition of C4-def-GPS and by untreated or heated, but not hydrazine treated GPS, and inhibited by zymosan treated GPS. IgG M-protein inhibits leucocyte adhesiveness and migration. Migration is specifically inhibited by the intact IgG, probably through complement activation with consumption of heat stable chemokinetic factor(s) and production of migration inhibitors.
D000818:Animals; D001667:Binding, Competitive; D001790:Blood Physiological Phenomena; D002464:Cell Migration Inhibition; D003181:Complement C4 / Q000172:deficiency; D006168:Guinea Pigs; D006493:Heparin / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism / Q000502:physiology*; D007957:Leukocyte Adherence Inhibition Test; D007962:Leukocytes / Q000276:immunology*; D009101:Multiple Myeloma / Q000276:immunology*
D003181:Complement C4; D007074:Immunoglobulin G; D006493:Heparin