[Radiosensitivity of pluripotent stem cells detectable by cloning in the spleen of irradiated mice].
It was shown that the dose--effect curves describing the radiosensitivity of CFUc of the bone marrow irradiated in vitro (0.04-3.7 Gy) and treated with normal rabbit serum (NRS) and anti-mouse-brain serum (AMBS) has two differently sloping portions indicating that two CFUc populations differing in radiosensitivity are present in the bone marrow. D0 was 0.93 Gy after irradiation with doses of 0.04-0.75 Gy and treatment with NRS, and 0.33 Gy after incubation of the bone marrow with AMBS. The addition of thymus cells "straightened" the dose--effect curve for the bone marrow treated with AMBS: in this case D0 was 1.81 Gy exceeding considerably the values of D0 for intact bone marrow. The CFUc population is suggested to be heterogeneous in radiosensitivity.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000528:radiation effects; D001921:Brain / Q000276:immunology; D002999:Clone Cells / Q000187:drug effects / Q000528:radiation effects; D003114:Colony-Forming Units Assay; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000528:radiation effects*; D007106:Immune Sera / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011817:Rabbits; D011836:Radiation Tolerance; D013154:Spleen / Q000187:drug effects / Q000528:radiation effects*
D007106:Immune Sera
Radiochuvstvitel'nost' pliuripotentnykh stvolovykh kletok, opredeliaemaia klonirovaniem v selezenke obluchennykh mysheĭ.