Preparation of deoxynivalenol (vomitoxin) from field-inoculated corn.
A process was developed for production of gram quantities of deoxynivalenol (DON) from corn that had been inoculated in the field with Fusarium graminearum and was estimated to contain 400-500 mg DON/kg. Steps in the purification procedure included extraction with methanol-water (1 + 1), partition from an aqueous solution into ethyl acetate by using a hydrophilic matrix, defatting, Florisil column chromatography, methylene chloride-water partition, semipreparative liquid chromatography (LC), and crystallization from ethyl acetate. The average yield of crystalline DON produced by this method (or with minor variations) was 281 mg/kg.
D002845:Chromatography / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D003460:Crystallization; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D012717:Sesquiterpenes / Q000302:isolation & purification*; D013055:Spectrophotometry, Infrared; D014255:Trichothecenes / Q000302:isolation & purification*; D003313:Zea mays / Q000032:analysis*
D012717:Sesquiterpenes; D014255:Trichothecenes; C007262:deoxynivalenol