Colloidal iron hydroxide staining of surface carbohydrates after glycerol treatment of uterine epithelial cells.
The structural relationships between intramembraneous particles (IMPs) and surface carbohydrates has been studied in uterine epithelial cells with a colloidal iron hydroxide (CIH) technique. To aggregate IMPs, glycerol treatment of unfixed cells was used and this treatment also caused some patching of CIH deposits on the cell surface. We conclude that some of the CIH receptors may be the surface expression of the IMPs.
D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D003102:Colloids; D004848:Epithelium / Q000032:analysis; D005260:Female; D005290:Ferric Compounds; D005990:Glycerol; D006651:Histocytochemistry / Q000379:methods*; D008854:Microscopy, Electron; D008871:Microvilli / Q000032:analysis; D051381:Rats; D014599:Uterus / Q000032:analysis* / Q000648:ultrastructure
D002241:Carbohydrates; D003102:Colloids; D005290:Ferric Compounds; C021024:ferric hydroxide; D005990:Glycerol