Neurotoxic esterase: gel filtration and isoelectric focusing of carboxylesterases solubilized from hen brain.
Carboxylesterase activity (EC 3.1.1.1) of hen brain including neurotoxic esterases NTEA and NTEB is solubilized from lyophilized lipid-extracted brain material by the use of n-octylglucoside. The solubilized enzymes are subjected to free isoelectric focusing, six carboxyl - esterase activity peaks are obtained. By gel filtration on Sephacryl S-300 neurotoxic esterases are separated from carboxylesterase isoenzymes V and X. The molecular weight of the neurotoxic esterases is estimated to be 1.8 X 10(6).
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000302:isolation & purification*; D002645:Chickens; D002850:Chromatography, Gel; D005260:Female; D007525:Isoelectric Focusing; D007700:Kinetics; D008970:Molecular Weight
D002265:Carboxylic Ester Hydrolases; C021859:neurotoxic esterase