Preparation and properties of monoclonal antibodies to the anabolic agent zeranol.
Monoclonal antibodies have been produced using two haptens, zeranol-7-hemisuccinate coupled to bovine serum albumin and zeranol-16-carboxypropyl ether coupled to human serum albumin. An assessment of cross-reactivity demonstrated that the monoclonal antibody raised against the 7-hemisuccinate derivative reacted with zeranol (100%), talernol (12%), zearalenone (17%), zearalanone (100%) and alpha- and beta-zearalenol (17% and less than 0.01%, respectively). In contrast the antibodies to the 16-carboxypropyl ether derivative reacted only with zeranol (100%) and also with a alpha-zearalenol (13-16%). All monoclonal antibodies were more specific than the polyclonal antibodies raised to the same haptens by conventional methods using sheep.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D002459:Cell Fusion; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D003429:Cross Reactions; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D008264:Macrophages; D051379:Mice; D008807:Mice, Inbred BALB C; D011863:Radioimmunoassay; D012118:Resorcinols / Q000276:immunology*; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000166:cytology; D015029:Zeranol / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D012118:Resorcinols; D015029:Zeranol