Noncollagenous phosphoprotein derived from teleostean fish-scales.
A noncollagenous phosphoprotein derived from teleostean fish-scales is extracted by the use of a dissociative extraction scheme, utilizing 4 M guanidine-HCl, pH 7.4, followed by a 4 M guanidine-HCl, 0.5 M EDTA, pH 7.4. DEAE-cellulose chromatography and repeated Sepharose CL-6B chromatography are used to purify this protein. The isolated protein contains extensive quantities of aspartic and glutamic acids in addition to O-phosphoserine and O-phosphothreonine. The apparent molecular weight of the phosphoprotein as determined by Sepharose CL-6B chromatography is 13 000.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002347:Carps / Q000502:physiology*; D003094:Collagen; D003530:Cyprinidae / Q000502:physiology*; D019791:Guanidine; D006146:Guanidines; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010750:Phosphoproteins / Q000302:isolation & purification*; D012867:Skin / Q000032:analysis*
D000596:Amino Acids; D006146:Guanidines; D010750:Phosphoproteins; D003094:Collagen; D019791:Guanidine