Evaluation of the acyclovir-induced modulation of the plaque-forming cell response of human peripheral blood lymphocytes.
The increasing use of the antiviral agent acyclovir has focused the attention to the possible side-effects on cellular and humoral immunological processes. The drug has preclinically been tested extensively but most investigations have been carried-out in animal models. The influence of acyclovir on the proliferation and the plaque-forming capability of pokeweed mitogen (PWM) activated cultures of unfractionated and isolated subpopulations from human peripheral blood lymphocytes (PBL), was investigated. These parameters were monitored with the 3H-thymidine incorporation technique and a protein A plaque-forming cell assay. Acyclovir had little inhibitory effect at physiological concentration whereas a detrimental decrease was noted at higher concentrations.
D000212:Acyclovir / Q000494:pharmacology*; D000328:Adult; D002469:Cell Separation; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000276:immunology*; D011043:Pokeweed Mitogens / Q000494:pharmacology; D010948:Viral Plaque Assay
D011043:Pokeweed Mitogens; D000212:Acyclovir