O-phenantroline-CuSO4-induced redistribution of nuclear proteins.
Incubation of rat liver nuclei with the o-phenantroline-CuSO4 (OP-Cu) complex under conditions not causing any DNA cleavage, enhanced the susceptibility of chromatin to the action of micrococcal nuclease. The released nucleosomal fraction had less coextracted nonhistone proteins, while the nuclear matrix was enriched in nonhistone proteins when compared with the controls. These changes were interpreted as the consequence of a displacement of nonhistone proteins from their closer association with the chromatin complex and a concomitant exposure of chromatin regions in a state less protected by nonhistone proteins.
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects* / Q000648:ultrastructure; D002843:Chromatin / Q000187:drug effects*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D003300:Copper / Q000494:pharmacology*; D019327:Copper Sulfate; D008099:Liver; D009707:Nucleosomes / Q000378:metabolism; D010618:Phenanthrolines / Q000494:pharmacology*; D051381:Rats; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D009707:Nucleosomes; D010618:Phenanthrolines; D013439:Sulfhydryl Reagents; D003300:Copper; D019327:Copper Sulfate