Observations on epidermal exsorption in mice following injections of procion dyes and ethidium bromide and topically applied dimethyl sulfoxide.
Back skin of hairless mice and external ears of CD-1 white mice were used to study the details of epidermal exsorption . Ethidium bromide, a DNA ligand, and two dichlorotriazinyl (procion) dyes were injected, i.v. or i.p. Migration patterns from the skin vasculature into the epidermis were observed by fluorescence microscopy and microspectrofluorometry . Topically applied DMSO greatly enhanced the exsorption process and produced intensely labeled epidermis. Ethidium bromide reacted primarily with nuclear DNA of living cells while the procion dyes tended to migrate intercellularly to label the stratum corneum. External ears of white mice treated topically with DMSO showed a pattern of labeling which included intense fluorescence of the cartilage and perichondrium as well as the ear epidermis.
D000287:Administration, Topical; D000818:Animals; D004396:Coloring Agents / Q000008:administration & dosage / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage / Q000494:pharmacology*; D004817:Epidermis / Q000187:drug effects* / Q000378:metabolism; D004996:Ethidium / Q000008:administration & dosage / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D051379:Mice; D008812:Mice, Hairless; D012869:Skin Absorption / Q000187:drug effects; D014227:Triazines / Q000008:administration & dosage / Q000378:metabolism*
D004396:Coloring Agents; D014227:Triazines; C041396:procion red MX-G; C041397:Procion Orange MX-2R; D004996:Ethidium; D004121:Dimethyl Sulfoxide