Leishmania major: culture media, mouse strains, and promastigote virulence and infectivity.
Promastigotes of Leishmania major were isolated from an infected mouse in two media, blood agar and Schneider's medium + 30% fetal calf serum, and maintained continuously for over 1 year. Infectivity studies in two strains of mice, outbred CD1 strain and inbred BALB/c strain, showed that promastigotes grown in Schneider's medium maintained infectivity to BALB/c mice throughout the period of cultivation. Infectivity to CD1 strain mice was progressively lost. Promastigotes grown in blood agar medium, however, lost infectivity to both strains of mice at a faster rate than promastigotes grown in Schneider's medium.
D000818:Animals; D006224:Cricetinae; D003470:Culture Media; D007891:Leishmania / Q000254:growth & development / Q000472:pathogenicity*; D007896:Leishmaniasis / Q000469:parasitology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D003470:Culture Media