title: De novo gene expression detected by amelogenin gene transcript analysis.
abstract: Reciprocal epithelial-mesenchymal interactions are responsible for mouse molar tooth organogenesis. Only dental ectomesenchymal cells are capable of instructing adjacent epithelial cells to become determined to synthesize and secrete enamel-specific proteins termed the amelogenins. To identify when inner enamel epithelial cells first express enamel specific gene products, cytoplasmic RNA has been analyzed from developing teeth by hybridization to a cloned cDNA probe to one of the amelogenins. It is reported that the de novo expression of amelogenin-encoding RNA as well as immunoprecipitated amelogenin polypeptides are first detected at Theiler stage 27. These data indicate that ectomesenchymal-mediated induction of inner enamel organ epithelia results in both the nascent transcription of amelogenin RNA and subsequent translation of amelogenin polypeptides, which are first detected at birth.
mesh_terms: D053523:Amelogenin; D000818:Animals; D001483:Base Sequence; D003746:Dental Enamel Proteins / Q000235:genetics*; D005260:Female; D005796:Genes; D051379:Mice; D008963:Molar / Q000196:embryology* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D014083:Tooth Germ / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D053523:Amelogenin; C507200:Amelx protein, mouse; D003746:Dental Enamel Proteins