Kinetic analyses of activation-induced changes in the hydrodynamic and surface properties of the glucocorticoid-receptor complex in mouse brain.
Unactivated, molybdate-stabilized, [3H]triamcinolone acetonide-labeled, glucocorticoid receptors from mouse whole brain were activated by removal of the molybdate and incubation at 22 degrees C for 1.5 to 24 min and then rapidly quenched at 0 degrees C with molybdate. The loss of the 9.2 S (unactivated) form of the [3H]TA-receptor complex and the concomitant formation of the 3.8 S (activated) form displayed first-order kinetics with a half-time of less than two min. The increase in the 3.8 S form correlated nearly perfectly with an increased binding to DNA-cellulose, and with a decreased and increased adsorption to DEAE-cellulose and glass fiber filters, respectively. The changes in adsorption to these filters, which occurred at a faster rate than did the changes in binding to DNA-C, are thought to reflect an increase in the relative number of positive charges and hydrophobic groups on the surface of the activated complex.
D000327:Adsorption; D000345:Affinity Labels; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002482:Cellulose / Q000031:analogs & derivatives / Q000378:metabolism; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003636:DEAE-Cellulose; D004247:DNA / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D005898:Glass; D007700:Kinetics; D046911:Macromolecular Substances; D051379:Mice; D008982:Molybdenum / Q000494:pharmacology; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D013499:Surface Properties; D014222:Triamcinolone Acetonide
D000345:Affinity Labels; C033190:DNA-cellulose; D046911:Macromolecular Substances; D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D008982:Molybdenum; D002482:Cellulose; D004247:DNA; D003636:DEAE-Cellulose; C024687:sodium molybdate(VI); D014222:Triamcinolone Acetonide