Partition of fatty acids and fluorescent fatty acids into membranes.
We have measured the membrane/water partition of free fatty acids, of a fluorescent analogue, and of chlorpromazine into plasma membranes from lymphoma, platelets, red cells, and liposomes of egg phosphatidylcholine. Three different methods were used: hygroscopic desorption [ Conrad , M.J., & Singer, S.J. (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 5202-5206; Conrad , M.J., & Singer, S.J. (1981) Biochemistry 20, 808-818], centrifugation, and fluorescence titration. The partition coefficients for chlorpromazine and all fatty acid/membrane combinations were in the range 10(2)-10(5). No significant partition difference was observed between liposomes and plasma membranes. These results suggest, therefore, that fatty acids and chlorpromazine can readily partition into artificial and cell plasma membranes.
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D002746:Chlorpromazine / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D005453:Fluorescence; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes; D008223:Lymphoma / Q000378:metabolism; D051379:Mice; D013229:Stearic Acids / Q000378:metabolism
D005230:Fatty Acids, Nonesterified; D008081:Liposomes; D013229:Stearic Acids; C026653:12-(9-anthroyloxy)stearic acid; D002746:Chlorpromazine