title: Isolation and transmission of Cowdria ruminantium (causal agent of heartwater disease) in Blue Nile Province, Sudan.
abstract: Heartwater was diagnosed in sheep at the Umm Banein Livestock Research Station, Blue Nile Province, Sudan. Cowdria ruminantium was isolated from sheep by subinoculation of blood into a goat. Adult Amblyomma lepidum ticks fed as nymphs on this goat transmitted heartwater to a Friesian (Bos taurus) calf. Brain stabilates from this calf, either snap-frozen into liquid nitrogen or frozen slowly in a deepfreezer at -70 degrees C, with DMSO as cryoprotectant, were infective to cattle when inoculated subcutaneously.
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology* / Q000635:transmission; D005260:Female; D006041:Goats / Q000382:microbiology*; D006357:Heartwater Disease / Q000382:microbiology* / Q000635:transmission; D008297:Male; D012287:Rickettsiaceae / Q000302:isolation & purification*; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology* / Q000635:transmission; D013397:Sudan; D013987:Ticks / Q000382:microbiology