Anti-laminin antibodies in Sprague-Dawley and brown Norway rats chronically exposed to cadmium.
Sprague-Dawley and Brown-Norway female rats were chronically exposed to cadmium. The metal was administered either in drinking water at a concentration of 20 or 100 ppm for 13 months or intraperitoneally (i.p.) at the dose of 1 mg/kg, 5 times a week for 4 months. Anti-laminin and anti-type IV procollagen antibodies (laminin and type IV procollagen are components of the glomerular basement membranes) were sequentially assessed by radioimmunoassay in serial serum samples. Anti-type IV procollagen antibodies were never detectable whereas anti-laminin antibodies were transiently found in the serum of Sprague-Dawley rats only. In the i.p. group, the antibodies were detected between week 4 and 8, whereas in the oral treatment group, they were detected between month 6 and 8. At the same time, the concentration of cadmium in kidney cortex averaged about 100 ppm in the 20 ppm cadmium group and about 200 ppm in the 2 other treatment groups. The pathogenic significance of these antibodies in chronic cadmium intoxication of Sprague-Dawley rats remains to be assessed since their occurrence was not associated with concomitant immunoglobulin deposits in the kidneys.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001485:Basement Membrane / Q000276:immunology; D002104:Cadmium / Q000633:toxicity*; D005260:Female; D007668:Kidney / Q000187:drug effects; D007678:Kidney Glomerulus / Q000276:immunology; D007797:Laminin / Q000276:immunology*; D011347:Procollagen / Q000276:immunology; D011507:Proteinuria / Q000139:chemically induced; D051381:Rats; D011919:Rats, Inbred Strains
D000906:Antibodies; D007797:Laminin; D011347:Procollagen; D002104:Cadmium