title: [Acid-soluble, non-histone proteins in rat liver chromatin. Interaction with DNA].
abstract: Some properties of nonhistone proteins of rat liver chromatin (Mr 40 +/- 1 and 41 +/- 1 KD) are described. These proteins are abundant in monomeric particles formed at the early steps of chromatin fragmentation by Ca2+,Mg2+-DNase. The proteins are not extracted from chromatin by 5% HClO4 and 1 M NaCl, but can be extracted by 0.4 n H2SO4 and 2 M NaCl. Study on proteins binding to DNA demonstrated that in 0.05 M NaCl these proteins are bound both to bovine satellite DNA and to the plasmid pBR 322 DNA.
mesh_terms: D000818:Animals; D001345:Autoradiography; D002843:Chromatin / Q000378:metabolism*; D002850:Chromatography, Gel; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004276:DNA, Satellite / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004706:Endodeoxyribonucleases / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D010957:Plasmids; D011485:Protein Binding; D051381:Rats
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004276:DNA, Satellite; D004247:DNA; D004706:Endodeoxyribonucleases; C029367:calcium magnesium dependent endodeoxyribonuclease
vernacular_title: Kislotorastvorimye negistonovye belki khromatina pecheni krys. Vzaimodeĭstvie s DNK.