Myosin heavy chain concentration, synthesis rate and degradation rate in normal and dystrophic chicken muscle cells in culture.
Myosin heavy chain concentrations, synthesis rates and degradation rates were studied in muscle cell cultures prepared from the breast muscle of 13-day normal (white leghorn) and dystrophic (line 307) chicken embryos. Muscle cells were studied after 7 days in culture, at which time they had reached a steady state with respect to myofibrillar protein synthesis and degradation. The quantity of myosin heavy chain was 10.5 +/- 0.9 micrograms/culture (n = 32) in normal cells and 8.10 +/- 1.2 micrograms/culture (n = 31) in dystrophic cells. However, the myosin heavy chain synthesis rate was 23 500 molecules/min/nucleus in normal cells and 39 900 molecules/min/nucleus in dystrophic cells, as determined by pulse labeling with [3H]leucine and measurement of the specific radioactivity of tRNA precursor pools. Myosin heavy chain half-lives rates were calculated to be 30.6 h in normal cells and 15.6 h in dystrophic cells when corrections were made for reutilization of [3H]leucine. Thus, dystrophic muscle cultures accumulate less myosin heavy chain, despite their faster synthesis rate, because of faster degradation of myosin heavy chain.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D009132:Muscles / Q000378:metabolism*; D009137:Muscular Dystrophy, Animal / Q000378:metabolism*; D009218:Myosins / Q000096:biosynthesis / Q000378:metabolism*; D014316:Tritium
D014316:Tritium; D009218:Myosins; D007930:Leucine