[Androgen binding by skeletal muscle nuclei].
A study was made of 3H-19-nortestosterone binding by isolated nuclei and 0.4 M KCl nuclear extract of the rat skeletal muscle. Binding specificity was ascertained by incubation in the presence of various unlabeled steroids. The Kd values were measured for nuclei and 0.4 M KCl nuclear extract (11.6 +/- 2.5 nM and 9.9 +/- 1.6 nM, respectively). The amount of binding sites was 24.1 +/- 1.7 fmol/mg DNA or 13.7 +/- 1.0 fmol/g tissue. Enzymatic treatment with pronase and DNase shows that nuclear androgen receptors are proteins. DNA was noted to have a stabilizing effect. DNase treatment of nuclei during extraction with 0.4 M KCl was shown to significantly increase the amount of specifically bound radioactivity in the extract.
D000728:Androgens / Q000378:metabolism*; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D008297:Male; D009132:Muscles / Q000378:metabolism*; D009277:Nandrolone / Q000378:metabolism; D051381:Rats; D011944:Receptors, Androgen / Q000378:metabolism; D013696:Temperature; D013739:Testosterone / Q000378:metabolism; D013997:Time Factors
D000728:Androgens; D011944:Receptors, Androgen; D013739:Testosterone; D009277:Nandrolone
Sviazyvanie androgenov iadrami skeletnykh myshts.