A simple rapid method for quantifying microorganisms by their metabolic activity when bound to a specific adsorbent.
A simple rapid assay for microorganisms is described. An adsorbent capable of binding microbial cells is deposited in a disposable plastic syringe. A sample to be analyzed is drawn into the syringe and the number of attached microorganisms is calculated from their ability to produce metabolites. The smallest number of yeast cells in a given sample was 50,000. Each analysis takes 2.5 h.
D003208:Concanavalin A / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007163:Immunosorbent Techniques; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000378:metabolism; D012685:Sepharose / Q000378:metabolism; D013053:Spectrophotometry
D003208:Concanavalin A; D012685:Sepharose