Factors affecting the consecutive and simultaneous coupling with bis-diazotized benzidine of protein antigen to sheep red blood cells.
The dynamics of attaching antigen to sheep red blood cells using bis-diazotized benzidine ( BDB ) were studied using purified protein derivative tuberculin as antigen. Factors considered were the concentration of antigen and BDB , temperature, time, and simultaneous or consecutive coupling. Simultaneous coupling at 42 degrees C for 10 min gave the most specific haemagglutination reactions and was simple to carry out.
D000818:Animals; D001560:Benzidines / Q000494:pharmacology*; D002498:Centrifugation; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology*; D006386:Hemagglutination Tests / Q000662:veterinary; D006863:Hydrogen-Ion Concentration; D012756:Sheep / Q000276:immunology; D013696:Temperature; D014373:Tuberculin / Q000276:immunology*
D001560:Benzidines; D014373:Tuberculin; C006770:bis(diazo)benzidine