Properties of hepatitis B e antigen synthesized by rat cells transfected with circular viral DNA.
Transfection of Buffalo rat liver cells with closed circular hepatitis B virus DNA resulted in the synthesis of both hepatitis B e and surface antigens. A 14000 mol. wt. peptide bearing hepatitis B e antigenic determinants was isolated from cell culture fluids. Native hepatitis B e antigen was present in multimeric forms in the cell culture fluids and was associated with protein phosphokinase activity. The multimeric forms of hepatitis B e antigen may serve both structural and enzymic functions for the hepatitis B virion with its small genome.
D000818:Animals; D002478:Cells, Cultured; D004270:DNA, Circular / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D006511:Hepatitis B Antigens / Q000235:genetics*; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006513:Hepatitis B e Antigens / Q000235:genetics* / Q000302:isolation & purification; D006515:Hepatitis B virus / Q000235:genetics*; D046911:Macromolecular Substances; D008970:Molecular Weight; D011494:Protein Kinases / Q000235:genetics; D051381:Rats; D014162:Transfection
D004270:DNA, Circular; D004279:DNA, Viral; D006511:Hepatitis B Antigens; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D046911:Macromolecular Substances; D011494:Protein Kinases