Failure to detect crystalline brushite in embryonic chick and bovine bone by X-ray diffraction.
We have reexamined the question of whether brushite (CaHPO4 X 2H2O) occurs in embryonic bone. On the basis of the experiments reported here, and a reexamination of data previously obtained in this laboratory, we have concluded that crystalline brushite as a separate phase identifiable by X-ray diffraction does not occur in embryonic chick or bovine bone. Crystalline brushite previously identified in this laboratory in the less-mineralized fractions of embryonic chick bone was apparently formed artifactually during the fractionation process, possibly from preexisting domains of HPO4(-2) groups in a noncrystalline brushite-like configuration.
D000818:Animals; D001842:Bone and Bones / Q000648:ultrastructure*; D002130:Calcium Phosphates / Q000032:analysis*; D002417:Cattle; D002642:Chick Embryo; D004622:Embryo, Mammalian; D010012:Osteogenesis; D013045:Species Specificity; D014961:X-Ray Diffraction
D002130:Calcium Phosphates; C494366:calcium phosphate, dibasic, dihydrate