The radioimmunoassay of alpha-melanocyte-stimulating hormone and endorphin in trout (Salmo gairdneri) and the effect of blinding on the plasma levels of these peptides.
Direct radioimmunoassays for alpha-melanocyte-stimulating hormone (alpha-MSH) and endorphin in trout plasma were established and validated. The equilibrium plasma levels of both alpha-MSH and endorphin were found to be significantly higher in blind than in intact trout maintained under identical conditions. These differences were accompanied by differences in body colour, the blind fish being very much darker than the intact fish.
D000818:Animals; D001766:Blindness / Q000503:physiopathology*; D003116:Color; D004723:Endorphins / Q000097:blood*; D009074:Melanocyte-Stimulating Hormones / Q000097:blood*; D009799:Ocular Physiological Phenomena; D011863:Radioimmunoassay / Q000379:methods; D012474:Salmon; D012487:Salmonidae / Q000097:blood*; D014337:Trout / Q000097:blood*
D004723:Endorphins; D009074:Melanocyte-Stimulating Hormones