title: The isolation and sequencing of human gastric inhibitory peptide (GIP).
abstract: Human GIP 1-42 and fragments of human GIP corresponding to GIP 10-42, GIP 11-42, and GIP 17-42 were isolated from acid-ethanol extracts of human small intestines with the aid of an anti-GIP serum specific for the extreme C-terminal portion of the GIP molecule. The full sequence of human GIP has been established by Edman degradation of these peptides and fragments thereof by automatic gas-phase sequencing. Human GIP differs from porcine GIP at residues 18 and 34. The sequence of human GIP is thus: (Formula: see text) Amino acid residues 18 and 34 are Arg and Ser, respectively, in porcine GIP.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005749:Gastric Inhibitory Polypeptide / Q000302:isolation & purification*; D005768:Gastrointestinal Hormones / Q000302:isolation & purification*; D006801:Humans; D007421:Intestine, Small / Q000032:analysis*; D010446:Peptide Fragments / Q000302:isolation & purification; D013552:Swine
chemical_list: D005768:Gastrointestinal Hormones; D010446:Peptide Fragments; D005749:Gastric Inhibitory Polypeptide