Production and characterization of a monoclonal antibody to rat liver thiol: protein-disulfide oxidoreductase/glutathione-insulin transhydrogenase.
Rat liver thiol:protein-disulfide oxidoreductase/glutathione-insulin transhydrogenase (glutathione:protein disulfide oxidoreductase, EC 1.8.4.2) was purified and found to give two bands on sodium dodecyl sulfate polyacrylamide gel electrophoresis. A monoclonal antibody was produced against this enzyme preparation and found to remove all the insulin degrading activity of purified preparations of the enzyme. This monoclonal antibody was also found to react with the two different forms of the enzyme observed on gel electrophoresis. These results suggest that glutathione-insulin transhydrogenase can exist in more than one state.
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D008099:Liver / Q000201:enzymology; D008970:Molecular Weight; D010088:Oxidoreductases / Q000276:immunology*; D011490:Protein Disulfide Reductase (Glutathione) / Q000276:immunology* / Q000302:isolation & purification; D051381:Rats
D000911:Antibodies, Monoclonal; D010088:Oxidoreductases; D011490:Protein Disulfide Reductase (Glutathione)