Inhibition of mRNA synthesis in rabbit bone marrow nuclei in vitro by quinone metabolites of benzene.
mRNA synthesis by rabbit bone marrow nuclei has been shown to be inhibited by the quinone metabolites of benzene, hydroquinone and p-benzoquinone, in a concentration-dependent manner with 50% inhibitory concentration (IC50[M]) for both compounds of 6 X 10(-6) M. Catechol and 1,2,4-benzenetriol also showed a concentration-dependent inhibition of synthesis, however, 50% inhibition was not reached by 10(-4) M. Phenol did not inhibit mRNA synthesis even at 10(-3) M. It is possible that myelotoxicity from benzene might result from such an inhibition of mRNA synthesis by quinone metabolites in pluripotent and/or committed bone marrow stem cells.
D000818:Animals; D016227:Benzoquinones; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism*; D002396:Catechols / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006873:Hydroquinones / Q000494:pharmacology*; D008297:Male; D011809:Quinones / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D011817:Rabbits; D014544:Uridine Triphosphate / Q000378:metabolism
D016227:Benzoquinones; D002396:Catechols; D006873:Hydroquinones; D011809:Quinones; D012333:RNA, Messenger; C013038:hydroxyhydroquinone; C004532:quinone; C034221:catechol; D014544:Uridine Triphosphate; C031927:hydroquinone