title: Characteristics of the phagocytically induced respiratory burst in leukocytes from young adult and aged beagle dogs.
abstract: Phagocytically stimulated canine leukocyte suspensions obtained from 12 young adult and 43 aged individuals were examined for several physiological manifestations of the phagocytically induced respiratory burst. There was considerable variation in levels of oxygen consumption, glucose oxidation via the hexose monophosphate shunt (HMPS), and chemiluminescence response by both resting and phagocytically stimulated leukocytes from different individual animals in each age-group. Leukocyte suspensions from specific individuals in each age-group exhibited a weakly responsive respiratory burst. Chronological age could not be used as a predictor of either the specific (oxygen consumption and HMPS activity) or nonspecific (chemiluminescence response) manifestations of the respiratory burst. The kinetics of the chemiluminescence response were similar for all age-groups. Collectively, the results suggest that there is not an age-related alteration in the phagocytically induced respiratory burst of canine neutrophils and that cells from young adult and aged dogs have a comparable capacity to generate levels of highly reactive antimicrobial oxidizing agents. The increased relative susceptibility of aging dogs to microbial agents is apparently not related to an absent, abbreviated, or reduced leukocyte respiratory burst.
mesh_terms: D000375:Aging; D000818:Animals; D004285:Dogs; D005260:Female; D006600:Hexosephosphates / Q000097:blood; D008163:Luminescent Measurements; D008297:Male; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D010101:Oxygen Consumption; D010587:Phagocytosis
chemical_list: D006600:Hexosephosphates