SDS-PAGE strongly overestimates the molecular masses of the neurofilament proteins.
Direct molecular mass determination of the three porcine neurofilament proteins (H, M and L) was performed in 6 M guanidine-HCl using analytical gel filtration and sedimentation equilibrium centrifugation. The results show that SDS-PAGE strongly overestimates the values of the 'higher molecular mass' components H and M. This discrepancy stems from the carboxyterminal extensions known to have unusual amino acid composition.
D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005189:False Positive Reactions; D007381:Intermediate Filament Proteins / Q000032:analysis*; D008970:Molecular Weight; D012967:Sodium Dodecyl Sulfate; D013552:Swine; D014461:Ultracentrifugation
D007381:Intermediate Filament Proteins; D012967:Sodium Dodecyl Sulfate