Inhibition of polyriboinosinic:polyribocytidylic acid-induced depression of mouse hepatic mixed function oxidases by actinomycin D or cycloheximide.
Administration of a single dose of the potent interferon inducer poly rI:rC to Swiss Webster mice depressed hepatic cytochrome P-450 to 75% of control, ethylmorphine N-demethylase to 56% of control and DMN N- demethylases I and II to about 80% of control. Although each enzyme responded in a unique manner, maximum depression occurred at 24 hours after poly rI:rC administration and the concurrent administration of inhibitors of protein synthesis (actinomycin D or cycloheximide) prevented this depression. These data suggest that poly rI:rC effects on the mixed function oxidases are not species specific although depression follows a time course shorter than that reported in the rat (maximum depression at 40 hours after poly rI:rC administration) and that depression occurs through the stimulation of a protein responsible for degrading cytochrome P-450.
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology*; D065607:Cytochrome P-450 Enzyme Inhibitors; D003609:Dactinomycin / Q000494:pharmacology*; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors*; D010089:Oxidoreductases, N-Demethylating / Q000037:antagonists & inhibitors; D011070:Poly I-C / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D065607:Cytochrome P-450 Enzyme Inhibitors; D003609:Dactinomycin; D003513:Cycloheximide; D006899:Mixed Function Oxygenases; D010089:Oxidoreductases, N-Demethylating; D011070:Poly I-C