Monoclonal antibodies which prevent experimental lung metastases. Interference with the adhesion of tumour cells to laminin.
Cellular adhesion is important during metastasis, as metastatic cells must escape from the primary site into lymph and blood systems, there to adhere specifically to sites in distant organs. We have recently selected monoclonal antibodies which prevent adherence of B16 mouse melanoma cells to tissue culture dishes, and also markedly reduce experimental lung metastasis in mice when injected before or with the tumor cells. Here, we investigated which step in the metastatic process may be affected by the antibodies. The possible inhibitory effect of antibody on tumour cell adherence to vascular endothelial monolayers and to purified components of the underlying extracellular matrix - fibronectin, laminin and collagen type IV - was studied using in vitro assays. We found that the antibodies significantly blocked attachment to laminin, suggesting that specific basement membrane components play an important role in attracting or otherwise modifying the behaviour of metastatic tumour cells.
D000818:Animals; D000911:Antibodies, Monoclonal; D002448:Cell Adhesion; D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D007797:Laminin / Q000378:metabolism*; D008175:Lung Neoplasms / Q000556:secondary*; D008545:Melanoma / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D013499:Surface Properties
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D007797:Laminin; D003094:Collagen