[Fluorometric analysis of the formation and repair of DNA breaks in irradiated cells].
A study was made of the dependence of the fluorescence of ethidium bromide upon NaOH concentration after staining of single- and double-strand DNA fragments. The possibility of the fluorometric estimation of the share of double-stranded DNA in cell lysates was demonstrated. The method of fluorometry was used to study the dose dependence of a change in the share of double-stranded DNA in the irradiated thymocytes and Ehrlich ascites carcinoma cells which permitted to determine the appearance and repair of DNA breaks in these cells.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D004247:DNA / Q000528:radiation effects*; D004260:DNA Repair / Q000528:radiation effects*; D004273:DNA, Neoplasm / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004996:Ethidium / Q000494:pharmacology; D005453:Fluorescence; D008297:Male; D051379:Mice; D012972:Sodium Hydroxide / Q000494:pharmacology; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*
D004273:DNA, Neoplasm; D012972:Sodium Hydroxide; D004247:DNA; D004996:Ethidium
Fluorimetricheskiĭ analiz poiavleniia i reparatsii razryvov v DNK obluchennykh kletok.