Selective elimination of fibroblasts from cultures of normal human melanocytes.
The main obstacle to establishing pure normal human melanocytes in vitro is contamination of the cultures by fibroblasts. The obstacle can be overcome by selective destruction of fibroblasts with geneticin ( G418 sulfate). Treatment of mixed cultures with this drug at a concentration of 100 micrograms/ml for two days results in pure cultures of normal human melanocytes.
D002455:Cell Division; D002469:Cell Separation / Q000379:methods; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D005839:Gentamicins / Q000633:toxicity; D006801:Humans; D007700:Kinetics; D008544:Melanocytes / Q000166:cytology* / Q000502:physiology
D003470:Culture Media; D005839:Gentamicins; C010680:antibiotic G 418