Antibody-mediated cellular cytotoxicity against Raji cells ADCC(Raji): evaluation of false positives in the detection of circulating immune complexes by Raji-cell assay.
Raji-cell radioimmunoassay is a very sensitive and reproducible method for the detection of circulating immune complexes. Using a complement-independent, antibody-dependent cellular cytotoxicity assay against 51Cr-labeled Raji cells, there is no correlation between activity against Raji cells and positivity in Raji-cell radioimmunoassay for circulating immune complexes in three sets of sera (from renal transplantation patients, multiparous women, and patients suffering from systemic lupus erythematosus). We conclude that IgG antibodies to Raji membranes are not a significant cause of false-positive results in circulating immune complexes as detected by Raji-cell radioimmunoassay.
D000920:Antibody-Dependent Cell Cytotoxicity; D000936:Antigen-Antibody Complex / Q000032:analysis*; D000961:Antilymphocyte Serum / Q000032:analysis; D002460:Cell Line; D005189:False Positive Reactions; D005260:Female; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007668:Kidney / Q000276:immunology; D016030:Kidney Transplantation; D007694:Killer Cells, Natural / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D011247:Pregnancy; D011961:Receptors, Fc
D000936:Antigen-Antibody Complex; D000961:Antilymphocyte Serum; D006680:HLA Antigens; D011961:Receptors, Fc