Sequential imaging of indium-111-labeled monoclonal antibody in human mammary tumors hosted in nude mice.
Using a bifunctional chelating agent, indium-111 was attached to a monoclonal antibody 10- 3D2 , specific for a 126-kilodalton phosphoglycoprotein antigen associated with human mammary carcinoma, and was then used to localize and visualize human mammary tumors hosted in nude mice. Simultaneous tumor concentration of In-111-10- 3D2 was eight times greater than that of control I-125-MOPC-21. Uptake of F(ab')2 and Fab of 10- 3D2 was also compared. the scintigrams demonstrated that intact antibody provided the best images. Control In-111-labeled MOPC-21 and plasma did not show specific localization in the tumor. Uptake of In-111-labeled 10- 3D2 was also compared in two lines of human mammary tumors, BT-20 and HS- 578T . Imaging with 10- 3D2 was better for BT-20 than for HS- 578T . These studies demonstrated that (a) In-111-10- 3D2 can be utilized to image human mammary tumors hosted in nude mice; (b) intact antibody provided the best tumor images, although F(ab')2 had optimal target-to-background ratios for earlier imaging; and (c) different mammary tumor lines with possibly different concentrations of tumor-associated antigen showed different rates of uptake and apparent saturation with 10- 3D2 .
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000302:isolation & purification; D000918:Antibody Specificity; D001943:Breast Neoplasms / Q000000981:diagnostic imaging*; D005260:Female; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000302:isolation & purification; D007136:Immunoglobulins / Q000008:administration & dosage; D007204:Indium; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods; D008325:Mammary Neoplasms, Experimental / Q000000981:diagnostic imaging*; D051379:Mice; D008819:Mice, Nude; D009194:Myeloma Proteins / Q000008:administration & dosage; D009368:Neoplasm Transplantation; D010750:Phosphoproteins / Q000276:immunology; D011868:Radioisotopes; D011877:Radionuclide Imaging
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D007140:Immunoglobulin Fab Fragments; D007136:Immunoglobulins; D007457:Iodine Radioisotopes; D009194:Myeloma Proteins; D010750:Phosphoproteins; D011868:Radioisotopes; C017551:myeloma immunoglobulins; D007204:Indium