Isolation of fibronectin under mild conditions.
Fibronectin was isolated from human blood plasma by affinity chromatography on immobilized Physiogel, a plasma expander made by chemical degradation of gelatin. Binding of fibronectin to Physiogel is weaker than to gelatin; elution could therefore be performed under rather mild conditions: at pH 6.5 and 30 degrees C. Loading of the sample at low temperature increases the capacity of the affinity column.
D002846:Chromatography, Affinity; D005353:Fibronectins / Q000302:isolation & purification*; D005780:Gelatin; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012685:Sepharose; D013696:Temperature
D005353:Fibronectins; D005780:Gelatin; D012685:Sepharose; C042021:physiogel