Determination of partial specific volume and absolute concentration by densimetry.
The apparent partial specific volume, phi, of a protein (or other macromolecule) and the absolute concentration, c, can be determined from the densimetry of solutions and diffusates in 1H2O , 2H2O and 1H2O/2H2O mixtures if the ratios of concentrations (relative concentrations) are known. The densimetry method allows a non-destructive check on total macromolecule concentrations without prior knowledge of specific u.v.-absorption coefficient. The method may be especially useful in small-angle neutron-scattering studies.
D002364:Caseins / Q000032:analysis; D003720:Densitometry; D003903:Deuterium; D046911:Macromolecular Substances; D008956:Models, Chemical; D011506:Proteins / Q000032:analysis*; D014867:Water
D002364:Caseins; D046911:Macromolecular Substances; D011506:Proteins; D014867:Water; D003903:Deuterium