title: Effect of atropine on the plasma cholecystokinin response to intraduodenal fat in man.
abstract: The present study was undertaken to determine whether atropine inhibits the plasma cholecystokinin (CCK) response to intraduodenal fat. Plasma CCK concentrations were measured by radioimmunoassay using two sequence-specific antibodies. Antibody 1703 bound to all COOH-terminal CCK peptides containing at least 14 amino acid residues, while antibody T204 was specific for the sulphated tyrosine region of CCK. Intraduodenal instillation of 60 ml corn oil in 6 normal subjects induced significant increases in plasma CCK. Intravenous administration of atropine (0.015 mg/kg as bolus followed by 0.005 mg/kg X h over 3 h) resulted in significant inhibition of plasma CCK concentrations at 10, 20 and 30 min (antibody 1703) and at 20 and 30 min (antibody T204 ) after instillation of fat. However, the peak increments in plasma CCK during atropine (8.6 +/- 1.9 pmol/l, antibody 1703; 5.4 +/- 1.1 pmol/l, antibody T204 ) were not different from those found without atropine (6.3 +/- 0.8 pmol/l, antibody 1703; 3.9 +/- 0.9 pmol/l, antibody T204 ). Similarly, the integrated plasma CCK secretion after intraduodenal fat was not significantly different when measured during atropine (461 +/- 119 pmol/l X 3 h, antibody 1703; 269 +/- 97 pmol/l X 3 h, antibody T204 ) and without atropine (428 +/- 62 pmol/l X 3 h, antibody 1703; 188 +/- 66 pmol/l X 3 h). It is concluded that administration of atropine delays but does not inhibit the CCK response to intraduodenal corn oil in man.
mesh_terms: D000328:Adult; D001285:Atropine / Q000494:pharmacology*; D002766:Cholecystokinin / Q000097:blood*; D003314:Corn Oil; D004386:Duodenum; D006339:Heart Rate / Q000187:drug effects; D006801:Humans; D007441:Intubation, Gastrointestinal; D008297:Male; D009821:Oils / Q000008:administration & dosage / Q000494:pharmacology*; D011863:Radioimmunoassay; D013268:Stimulation, Chemical
chemical_list: D009821:Oils; D001285:Atropine; D003314:Corn Oil; D002766:Cholecystokinin