Simultaneous determination of penicillin and cephalosporin antibiotics in serum by gradient liquid chromatography.
We describe a "high-performance" liquid-chromatographic method for simultaneously measuring various penicillin and cephalosporin antibiotics. After extraction from serum, which in general is quantitative, the drugs are separated by use of a "Bondapak phenyl" column and a gradient mobile phase. For these drugs retention times depend on the pH of the mobile phase; we present retention times under selected pH conditions.
D000900:Anti-Bacterial Agents / Q000302:isolation & purification; D002511:Cephalosporins / Q000097:blood* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005189:False Positive Reactions; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010406:Penicillins / Q000097:blood* / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D010406:Penicillins