Regulation of pituitary DNA synthesis during different reproductive states in the female rat: role of estrogens and prolactin.
We studied DNA synthesis in the rat adenohypophysis during the estrous cycle, pregnancy and lactation. During the estrous cycle, DNA synthesis was 3 times higher on the morning of estrus than on the other days. This peak was abolished completely by ovariectomy or pentobarbital, which also blocked the preovulatory surges of LH and prolactin. Methallibure , which blocked the LH but not the prolaction surge, had a partial effect on DNA synthesis. An acute and significant decrease in pituitary DNA synthesis occurred between days 0 (estrus) and 1 of pregnancy, followed by a less pronounced diminution until parturition. After delivery, DNA synthesis increased steeply on day 1 of lactation, returning to low values by day 3, under normal suckling conditions. Thelectomy , which blocked suckling-induced prolactin release, or antiestrogen treatment, which did not decrease prolactin secretion, diminished pituitary DNA synthesis on day 1 of lactation. Estrogen administration to intact or ovariectomized rats on days 9-11 of lactation stimulated (100%) DNA synthesis. Ovariectomy had no effect. In conclusion, in the different reproductive states studied, pituitary DNA synthesis is related to prolactin release in the presence of estrogens.
D000818:Animals; D002369:Castration; D004261:DNA Replication / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000502:physiology*; D004971:Estrus; D005260:Female; D007774:Lactation; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011247:Pregnancy; D011388:Prolactin / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains; D013629:Tamoxifen / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism
D004967:Estrogens; D013629:Tamoxifen; D004958:Estradiol; D011388:Prolactin; D013936:Thymidine