Chemical and ultrastructural differences in endotoxic glycolipids from Salmonella minnesota Re mutant extracted with various solvent systems.
Endotoxic glycolipids ( ReGl ) extracted from the whole cells (WC) and cell walls of heptose-less Re mutant of Salmonella minnesota with hot phenol-water (PW), phenol-chloroform-petroleum ether (PCP), and chloroform-methanol (CM) were analyzed chemically and examined with an electron microscope. ReGl -PW-(WC) contained mannose and proteins as contaminants, ReGl -PCP(WC) consisted of an excess amount of amino compound (cadaverine), and ReGl -PCP(WC) consisted of proteins and cadaverine, in addition to the ReGl constituents. The ultrastructure of ReGl -PW(WC) resembled onions when stained with uranyl formate, and was spherical when stained with uranyl acetate, sodium phosphotungstate and ammonium molybdate, whereas those of ReGl -PCP(WC) and ReGl -CM(WC) were shaped like ribbons. However, the shadowed ultrastructures of ReGl -PCP(WC) and ReGl -CM(WC) showed small pieces of flat and wide sheets, respectively. ReGl -PCP(WC) re-extracted by the PW method was found to be converted into the onion-like structure which was similar to that of ReGl -PW(WC), while an intermediate form (fingerprint-like) was observed after re-extraction of ReGl -PW(WC) with PCP. It was strongly suggested that the ultrastructural arrangement of ReGl was dependent on the solvent systems used for extraction.
D000473:Alkanes; D001427:Bacterial Toxins / Q000032:analysis* / Q000302:isolation & purification; D055598:Chemical Phenomena; D002621:Chemistry; D002725:Chloroform; D004731:Endotoxins / Q000032:analysis* / Q000302:isolation & purification; D004768:Enterotoxins / Q000032:analysis* / Q000302:isolation & purification; D006017:Glycolipids / Q000032:analysis* / Q000302:isolation & purification; D000432:Methanol; D008854:Microscopy, Electron; D019800:Phenol; D010636:Phenols; D012475:Salmonella; D014867:Water
D000473:Alkanes; D001427:Bacterial Toxins; D004731:Endotoxins; D004768:Enterotoxins; D006017:Glycolipids; D010636:Phenols; C027952:salmonella toxin; D014867:Water; D019800:Phenol; D002725:Chloroform; C004544:naphtha; D000432:Methanol