title: Oligosaccharide structures mediating agglutination of sheep erythrocytes by Staphylococcus saprophyticus.
abstract: Agglutination of sheep erythrocytes by Staphylococcus saprophyticus was used as a model system for adherence studies. Glycolipids were isolated from sheep erythrocyte membranes, and oligosaccharides were prepared by trifluoroacetolysis. The oligosaccharides were characterized by sugar analyses, methylation analyses, gas-liquid chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy. We showed that oligosaccharides containing terminal beta-D-galactose-p-(1-4)-beta-D-2-acetamido-2-deoxyglucose-p-(1- were good inhibitors of the hemagglutination of sheep erythrocytes by S. saprophyticus.
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004910:Erythrocyte Membrane / Q000276:immunology*; D006017:Glycolipids / Q000097:blood* / Q000302:isolation & purification; D006384:Hemagglutination; D009844:Oligosaccharides / Q000276:immunology*; D012756:Sheep; D013210:Staphylococcus / Q000276:immunology*; D013329:Structure-Activity Relationship
chemical_list: D006017:Glycolipids; D009844:Oligosaccharides