title: Identification of plasma proteins containing sulfite-reactive disulfide bonds.
abstract: Plasma protein S-sulfonate compounds (RS-SO-3) have previously been shown to form, presumably by sulfitolysis of disulfide bonds, as a result of exposure to sulfite. In the investigations reported here, we identify two proteins in rabbit plasma, namely albumin and plasma fibronectin, which contain reactive sites for S-sulfonate formation. Separation and identification of these proteins following in vitro and in vivo exposure to sulfite was accomplished primarily by column chromatographic and electrophoretic techniques. In addition, the structure of presumed S-sulfonate groups was confirmed by the identification of cysteinyl-S-sulfonate residues in protein hydrolysates generated by enzymatic digestion. The molar ratio of RS-SO-3 in both albumin and plasma fibronectin was less than one. Data from our experiments suggest that the mixed disulfide site of non- mercaptalbumin is the reactive site for S-sulfonate formation. The site(s) of formation within the plasma fibronectin molecule was not investigated. The possible physiological significance of disulfide sulfitolysis of albumin and plasma fibronectin is discussed.
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D004220:Disulfides / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000097:blood*; D046911:Macromolecular Substances; D008970:Molecular Weight; D011817:Rabbits; D012709:Serum Albumin / Q000378:metabolism*; D013447:Sulfites / Q000097:blood*; D013451:Sulfonic Acids / Q000097:blood*; D013462:Sulfur Radioisotopes
chemical_list: D004220:Disulfides; D005353:Fibronectins; D046911:Macromolecular Substances; D012709:Serum Albumin; D013447:Sulfites; D013451:Sulfonic Acids; D013462:Sulfur Radioisotopes