title: Acid phosphatase localization in normal and dystrophic retinal pigment epithelium.
abstract: In this study acid phosphatase (ACPase) was localized in the retinal pigment epithelium (RPE) of normal and Royal College of Surgeons (RCS) rats pink-eyed and pigmented with inherited retinal dystrophy to determine differences in staining during the post-engulfment stages of phagocytosis using two substrates, Na-beta-glycerophosphate and cytidine-5'-monophosphate. Staining was similar using either substrate and in the normal RPE the Golgi system, lysosomes and phagosomes were ACPase-positive. In the dystrophic RPE, which has a diminished capacity to phagocytose photoreceptor rod outer segments, ACPase staining was localized on melanosomes in the pigmented dystrophic and on the apical microvillous membranes in the pink-eyed dystrophic, but was not localized on phagosomes in either the pink-eyed or pigmented dystrophic RPE. Since only a few phagosomes were seen at any given time in dystrophic RPE in vivo, a tissue explant system was used to examine the number of latex beads phagocytosed by normal and RCS RPE, as well as the number of phagosomes containing both beads and ACPase activity in the normal and mutant RPE. Our findings indicate that in the dystrophic, fewer phagosomes are ACPase-positive than in the normal, and that some enzyme may be inappropriately shunted to either the apical microvilli or to melanosomes instead of to phagolysosomes.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D003568:Cytidine Monophosphate; D005994:Glycerophosphates; D006651:Histocytochemistry; D008854:Microscopy, Electron; D009940:Organoids / Q000201:enzymology; D010586:Phagocytes / Q000201:enzymology*; D010857:Pigment Epithelium of Eye / Q000201:enzymology*; D051381:Rats; D012162:Retinal Degeneration / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D005994:Glycerophosphates; D000135:Acid Phosphatase; D003568:Cytidine Monophosphate; C031463:beta-glycerophosphoric acid