title: The role of Na+ and Ca2+ ions on the action of pancreatic lipase studied with the help of immobilisation techniques.
abstract: The role of Na+ and Ca2+ as well as the influence of other metal ions on the lipase action were studied with the aid of immobilisation techniques. By this method it is possible to distinguish between the action of these ions on the lipase molecule itself and their action on the substrate or product. It could be shown that both alkali and alkali earth metal ions, especially Na+, Ca2+ and Mg2+ stabilize active states of the enzyme which were detected by immobilization to controlled pore glass beads. Though Na+ as well as Ca2+ and Mg2+ stabilize the enzyme significantly, they differ in their efficacy. Reasons for this are discussed and the data compared to the findings of other authors who performed their studies with the native enzyme.
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology*; D002413:Cations, Divalent / Q000502:physiology; D002414:Cations, Monovalent / Q000502:physiology; D004800:Enzymes, Immobilized; D008049:Lipase / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology*; D012964:Sodium / Q000502:physiology*; D013379:Substrate Specificity; D013552:Swine; D014215:Triacetin / Q000378:metabolism
chemical_list: D002413:Cations, Divalent; D002414:Cations, Monovalent; D004800:Enzymes, Immobilized; D012964:Sodium; D008049:Lipase; D002118:Calcium; D014215:Triacetin