[Plasma determination of paracetamol using high performance liquid chromatography. Application to a pharmacokinetic study].
A plasma assay for paracetamol by HPLC is described. Paracetamol is extracted with diethyl ether in the presence of an internal standard, beta- hydroxyethyltheophylline . The extracts are analysed using reversed phase chromatography (Radial Pak C18 column), the mobile phase consisting of a buffer mixture of 0.01 mol/l acetate (pH 4)-acetonitrile (920-80, v/v). The method is simple, rapid, precise and sensitive (lower level of sensitivity 0.13 mumol/l). It was used in a pharmacokinetic study in twelve volunteers.
D000082:Acetaminophen / Q000097:blood* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007700:Kinetics
D000082:Acetaminophen
Dosage plasmatique du paracétamol par chromatographie liquide haute performance. Application à une étude pharmacocinétique.