Acyl-chain specificity and membrane fluidity. Factors which influence the activity of a purified phospholipid-transfer protein from lung.
A purified phospholipid-transfer protein from rat lung has been characterized in terms of the specificity of the protein for phosphatidylcholine molecules with different apolar moieties. The study demonstrated that the lung-phospholipid-transfer protein discriminates between dipalmitoylphosphatidylcholine and molecular species of phosphatidylcholine with unsaturated acyl chains. The initial rate of transfer of dipalmitoylphosphatidylcholine is 1.5-fold greater than the rate of transfer of dioleoylphosphatidylcholine, 1-palmitoyl-2- arachidonylphosphatidylcholine , or egg phosphatidylcholine under most assay conditions. Although the protein preferentially transfers dipalmitoylphosphatidylcholine, the incorporation of increasing mole percentages of dipalmitoylphosphatidylcholine into unilamellar phosphatidylcholine vesicles profoundly affects their effectiveness as donors for phosphatidylcholine transfer by the transfer protein. At 60 mol% dipalmitoylphosphatidylcholine, the rate of transfer is one-third that observed when vesicles are composed of 100% egg phosphatidylcholine. Decreases in membrane fluidity as estimated by fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene correlate with decreases in the effectiveness of the vesicles as donors in the phospholipid-transfer reaction. The conclusion from these studies is that the rate of transfer of phosphatidylcholine by the purified phospholipid-transfer protein from lung is determined by physical properties of membrane interfaces with which the protein interacts, as well as by the specificity of the phospholipid-transfer protein for different molecular species of phosphatidylcholine.
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D008081:Liposomes; D008168:Lung / Q000378:metabolism*; D008560:Membrane Fluidity; D008565:Membrane Proteins; D008856:Microscopy, Fluorescence; D008930:Mitochondria, Liver / Q000378:metabolism; D010713:Phosphatidylcholines; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D013379:Substrate Specificity; D013816:Thermodynamics
D002352:Carrier Proteins; D008081:Liposomes; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids