Monoclonal antibodies specific for beta-adrenergic ligands.
After somatic cell fusion between splenocytes of immunized BALB/c mice and NS-1 myeloma cells, eight clones were obtained secreting anti-alprenolol antibodies as characterized by means of an ELISA. Four of these were subcloned and were studied further. The association constant for alprenolol ranged from 1.9 X 10(6) M-1 to 24 to 10(6) M-1. Competitive inhibition of [3H]-l-dihydroalprenolol binding revealed cross-reactivity with beta-adrenergic ligands, with a higher avidity for antagonists than for agonists. Two of the antibodies had a higher affinity for the l-isomer than for the d-isomer. The most stereospecific of these antibodies showed only affinity for beta-adrenergic antagonists and for the agonist isoproterenol. The other recognized both beta-adrenergic antagonists and agonists; it also showed an increase in tryptophan fluorescence after ligand binding. This property was used for the physicochemical study of the hapten-antibody interaction.
D000526:Alprenolol / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000096:biosynthesis; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D004082:Dihydroalprenolol / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011433:Propranolol / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D000911:Antibodies, Monoclonal; D004082:Dihydroalprenolol; D000526:Alprenolol; D011433:Propranolol