[Isolation and purification of specific proteins, forming during endometrium differentiation into decidual tissue].
Using analytical PAAG-electrophoresis two specific proteins, D1 and D2, were found in decidual tissue of pregnant women (6-12 weeks). These proteins were isolated and purified by means of ammonium sulfate saturation, chromatography on Sepharose 6B and ion exchange chromatography on DEAE 52 cellulose. The degree of purification of both D1 and D2 proteins was 100% and 81%, respectively. The both proteins were free of DNA, RNA and polysaccharides. The pI values of D1 and D2 proteins were at pH 5.6-6.0. According to gel electrophoresis on PAA-SDS-Na and gel filtration on Sepharose 6B, molecular weight of D1 protein was about 50,000-55,000 and of D2 protein--150,000-160,000 and 300,000-320,000, respectively. The specific decidual proteins D1 and D2 appear to be distinct from the so-called "pregnancy proteins" described previously.
D002852:Chromatography, Ion Exchange; D003656:Decidua / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004717:Endometrium / Q000502:physiology; D005260:Female; D006801:Humans; D008970:Molecular Weight; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000302:isolation & purification*; D011261:Pregnancy Trimester, First
D011257:Pregnancy Proteins
Vydelenie i ochistka spetsificheskikh belkov, obrazuiushchikhsia pri differentsirovke éndometriia v detsidual'nuiu tkan'.