The differential expression of the actins and tubulins during spermatogenesis in the mouse.
Following intratesticular injection of [35S]methionine, the multiple isoforms of actin and tubulin from highly purified mouse testicular meiotic and post-meiotic cells have been analysed by high resolution two-dimensional gel electrophoresis. In pachytene spermatocytes both beta and gamma actin are synthesized, gamma actin being made in a significantly greater amount. The relative proportion of synthesis of beta and gamma actin changes during spermiogenesis, beta actin increasing and gamma actin decreasing in round spermatids, elongating spermatids, and residual bodies. Both alpha and beta tubulin are synthesized in approximately equal proportion in pachytene spermatocytes. In addition to the tubulin isoforms synthesized during meiosis, at least one new form of both alpha and beta tubulin first appears in post-meiotic (haploid) cells. In elongating spermatids and residual bodies, the synthesis of alpha tubulin is drastically reduced.
D000199:Actins / Q000096:biosynthesis* / Q000302:isolation & purification; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008540:Meiosis; D008715:Methionine / Q000378:metabolism; D051379:Mice; D013091:Spermatogenesis; D013462:Sulfur Radioisotopes; D013737:Testis / Q000502:physiology*; D014404:Tubulin / Q000096:biosynthesis* / Q000302:isolation & purification
D000199:Actins; D013462:Sulfur Radioisotopes; D014404:Tubulin; D008715:Methionine