Thyromimetic effects of 3,5-dimethyl,3'-isopropyl thyronine (DIMIT) and 3,5-diethyl,3'-isopropyl thyronine (DIET) in various tissues of the rat.
We have examined the effects of treatment of the rat with 3,5-dimethyl-3'-isopropyl thyronine (DIMIT) and 3,5-diethyl-3'-isopropyl thyronine (DIET) on serum thyrotropin (TSH) concentration, heart weight, hepatic outer ring (5'-) monodeiodination of T4 to T3, cardiac outer ring monodeiodination of 3'-5'-diiodothyronine (3',5'-T2) to 3'-monoiodothyronine (3'-T1), and cerebral cortical monodeiodination of 3,5-T2 in the inner ring to 3-T1. Groups of four to seven rats were injected intraperitoneally either once a day or at eight-hour intervals for three days with saline or thyronines. Serum TSH was measured by radioimmunoassay. The various monodeiodinations were studied in homogenates of the tissues at optimal pH (7.35 for outer ring monodeiodinations and 8.0 for inner ring monodeiodination) and temperature (37 degrees C) in the presence of an excess of dithiothreitol. DIMIT was clearly active in all thyromimetic effects studied. It was about 10% as active as T4 in suppression of TSH. It stimulated hepatic monodeiodination of T4 to T3 in a similar manner to T4. DIMIT was also more comparable to T4 than to T3 in effect on heart weight and cardiac metabolism of 3',5'-T2 to 3'-T1. It was highly active, apparently more so than either T4 or T3, in cerebral cortical stimulation of metabolism of 3,5-T2 to 3-T1. In distinction to DIMIT, DIET had very little thyromimetic activity.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D001711:Biotransformation; D002540:Cerebral Cortex / Q000378:metabolism; D004104:Diiodothyronines / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D013963:Thyroid Hormones / Q000378:metabolism*; D013970:Thyronines / Q000378:metabolism / Q000494:pharmacology*; D013972:Thyrotropin / Q000097:blood*; D013974:Thyroxine / Q000378:metabolism; D014284:Triiodothyronine / Q000096:biosynthesis
D004104:Diiodothyronines; D013963:Thyroid Hormones; D013970:Thyronines; D014284:Triiodothyronine; C013068:3-monoiodothyronine; C022861:3,5-dimethyl-3'-isopropyl-L-thyronine; C019017:3'-monoiodothyronine; C030103:3,5-diiodothyronine; C030102:3',5'-diiodothyronine; C030543:3,5-diethyl-3'-isopropylthyronine; D013972:Thyrotropin; D013974:Thyroxine