Determination of tyrosine exposure in proteins by second-derivative spectroscopy.
The mutual interference between the second-derivative bands of tyrosine and tryptophan in proteins has been evaluated in terms of the ratio r between two peak to peak distances. The r values have been found to be not only related to the tyrosine/tryptophan ratio but also dependent on the polarity of the medium in which tyrosyl residues are embedded. The results obtained on purified proteins have been found consistent with the available X-ray information and with the existing solvent perturbation data.
D000107:Acetylation; D000818:Animals; D011487:Protein Conformation; D011506:Proteins; D013056:Spectrophotometry, Ultraviolet; D014364:Tryptophan / Q000032:analysis; D014443:Tyrosine / Q000032:analysis*
D011506:Proteins; D014443:Tyrosine; D014364:Tryptophan