Concanavalin A binding glycoproteins in subcellular fractions from the developing rat cerebral cortex.
Synaptic plasma membrane (SPM) and mitochondrial fractions were prepared from 3-50-day rat cerebral cortex and their purity assessed. The fractions were subjected to electrophoresis on slab gels, stained for protein, and overlaid with 125I-concanavalin A (ConA). ConA binding glycoproteins (CABGs) were revealed by autoradiography. In the SPM fraction CABGs of MW 25,000, 63,000, 80,000, 115,000, 174,000, and 239,000 increased while those of MW 47,000, 75,000, and 190,000 decreased developmentally. In the mitochondrial fraction, CABGs of MW 25,000, 44,000, 115,000 and 174,000 increased while those of 34,000, 43,000, 47,000, 51,000, 80,000, 107,000, and 195,000 decreased developmentally. CABGs of MW 32,000, 63,000, 88,000, 153,000, 190,000, and 239,000 appear to be unique to the SPM fraction and those of MW 34,000, 107,000, and 195,000 are unique to the mitochondrial fraction.
D000375:Aging; D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification*; D002540:Cerebral Cortex / Q000254:growth & development*; D004591:Electrophoresis, Polyacrylamide Gel; D008928:Mitochondria / Q000032:analysis; D008970:Molecular Weight; D051381:Rats; D011952:Receptors, Concanavalin A / Q000302:isolation & purification*; D013347:Subcellular Fractions / Q000032:analysis; D013570:Synaptic Membranes / Q000032:analysis
D002352:Carrier Proteins; D011952:Receptors, Concanavalin A; C027774:concanavalin A-binding glycoproteins