title: Effect of silver ions on transport and retention of phosphate by Escherichia coli.
abstract: Silver ions inhibited phosphate uptake and exchange in Escherichia coli and caused efflux of accumulated phosphate as well as of mannitol, succinate, glutamine, and proline. The effects of Ag+ were reversed by thiols and, to a lesser extent, by bromide. In the presence of N-ethylmaleimide and several uncouplers, Ag+ failed to cause phosphate efflux, but still inhibited exchange of intracellular and extracellular phosphate, indicating an interaction at more than one site. It is unlikely that Ag+ caused metabolite efflux by acting solely as an uncoupler, as an inhibitor of the respiratory chain, or as a thiol reagent.
mesh_terms: D001692:Biological Transport / Q000187:drug effects; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D005033:Ethylmaleimide / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism*; D012835:Silver Nitrate / Q000494:pharmacology*; D013431:Sulfates / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D013696:Temperature; D014220:Trialkyltin Compounds / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology; D019287:Zinc Sulfate
chemical_list: D010710:Phosphates; D013431:Sulfates; D013438:Sulfhydryl Compounds; D014220:Trialkyltin Compounds; C011559:tributyltin; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D019287:Zinc Sulfate; D012835:Silver Nitrate; D015032:Zinc; D005033:Ethylmaleimide