title: The effect of testosterone on the release of endogenous catecholamines from the hypothalamus of the cockerel in vitro.
abstract: Castration of immature cockerels caused a significant rise in plasma luteinizing hormone concentrations. This effect was prevented by testosterone (T) replacement therapy. In vivo T replacement therapy reduced the release of endogenous catecholamines from hypothalamic tissue following in vitro incubation with high K+ artificial avian cerebrospinal fluid (ACSF). In vitro incubation with 500 nM T significantly reduced the release of endogenous catecholamines from hypothalamic tissue following incubation with high K+ ACSF. These results suggest that T can reduce the functional activities of catecholaminergic neurones in the hypothalamus and that these neurones may play a facilitatory role in the control of gonadotrophin secretion in the cockerel.
mesh_terms: D000818:Animals; D002369:Castration; D002395:Catecholamines / Q000378:metabolism*; D002645:Chickens; D004298:Dopamine / Q000378:metabolism; D004837:Epinephrine / Q000378:metabolism; D007031:Hypothalamus / Q000187:drug effects* / Q000378:metabolism; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D009638:Norepinephrine / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D002395:Catecholamines; D013739:Testosterone; D007986:Luteinizing Hormone; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine