Synthesis of diadenosine 5',5'''-P1,P4-tetraphosphate (AP4A) in sea urchin embryos.
Sea urchin embryos were labeled with [3H]adenosine at two developmental stages (morula and prism) and the labeled acid-soluble nucleotides were fractionated successively by column chromatography with DEAE-Sephadex and DEAE-cellulose, and by thin-layer chromatography on a PEI-cellulose plate. Significant radioactivity was detected on the PEI-cellulose plate at the region of diadenosine 5',5'''-P1,P4-tetraphosphate (AP4A). After treatment of this fraction with phosphodiesterase, the radioactivity was all recovered in the AMP region, while alkaline phosphatase had no effect on the AP4A fraction. The present result suggests that AP4A is actively synthesized in the sea urchin embryos.
D000227:Adenine Nucleotides / Q000096:biosynthesis* / Q000302:isolation & purification; D000241:Adenosine / Q000378:metabolism; D000818:Animals; D015226:Dinucleoside Phosphates; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D005260:Female; D009028:Morula / Q000378:metabolism; D012617:Sea Urchins / Q000378:metabolism*; D014316:Tritium
D000227:Adenine Nucleotides; D015226:Dinucleoside Phosphates; D014316:Tritium; C020733:diadenosine tetraphosphate; D000241:Adenosine