[Effect of chitosan on the electrophoretic motility of thymocytes].
An increase of contact interaction between macrophages and thymocytes in the presence of polysaccharide chitosan was suggested to be due to the change of surface charge of interacting cells. It was found that incubation of thymocytes in the presence of chitosan leads to the change of their charge in the positive direction. The measurement of electrophoretic mobility of the cells was carried out on " Parmoquant 2" (Carl Zeiss Jena, DDR). The change of electrophoretic mobility increases with decrease of pH, increase of chitosan concentration and decrease of the cell concentration in the medium. This phenomenon is probably due to absorption of positively charged molecules of chitosan on negatively charged cell surface.
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology; D048271:Chitosan; D004305:Dose-Response Relationship, Drug; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D051379:Mice; D008808:Mice, Inbred CBA; D013950:Thymus Gland / Q000166:cytology*
D002686:Chitin; D048271:Chitosan
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