title: The use of projections for dimensionality reduction of flow cytometric data.
abstract: In order to visualize multiparameter flow cytometric measurements it is desirable to reduce the dimensionality of the data to two while preserving important features of each data pattern. We describe a method that projects two-parameter data onto a straight line that forms an angle theta with one of the original parameter axis. The angle is selected interactively or by principal component analysis. The procedure is applied on-line or off-line to pairs of parameters of the original data set that has been collected in LIST mode.
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D003201:Computers; D003626:Data Display; D005434:Flow Cytometry / Q000295:instrumentation*; D051379:Mice