Hybridoma preservation at -70 degrees C: a simple and economical procedure for the short-term storage and individual recovery of hybridomas.
A simple, economical, and time-saving procedure for freezing freshly prepared hybridomas at--70 degrees C is described. The procedure allows for supernatant fluid collection and storage of hybridomas in 96-well flexible polyvinyl microtiter plates. As the plates are sealed, and the plate is relatively soft, individual wells can be removed and cells recovered at the investigator's discretion. Consequently, this procedure allows significant latitude with regard to the time-consuming steps usually associated with hybridoma screening, expansion, and sub-cloning techniques.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D000937:Antigen-Antibody Reactions; D001428:Bacterial Vaccines / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005615:Freezing; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D011309:Preservation, Biological / Q000379:methods*; D013291:Streptococcus / Q000276:immunology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D001428:Bacterial Vaccines