Determination of amphetamine, norephedrine, and their phenolic metabolites in rat brain by gas chromatography.
A specific analytical procedure for the quantitation of amphetamine (I), norephedrine (III), and their amphoteric metabolites, p-hydroxy-amphetamine (II) and p-hydroxynorephedrine (IV), in biological samples using electron-capture gas chromatography (GC-EC) is described. The procedure utilizes the ion-pairing reagent, bis(2-ethylhexyl)phosphoric acid, which frees the amines from most contaminants and permits the efficient extraction of the amphoteric compounds (as acetates) from the aqueous solution. Amines I and III and acetylated amines II and IV were perfluoroacetylated prior to GC-EC analysis. Metabolism of I, II, and III in the rat brain was studied. Results indicate that both in vivo and in vitro amines I and III are p-hydroxylated to II and IV, respectively, and II is beta-hydroxylated to give IV. Norephedrine (III) was not detected as a rat brain metabolite of amphetamine (I).
D000661:Amphetamine / Q000032:analysis*; D000818:Animals; D001711:Biotransformation; D001923:Brain Chemistry; D002849:Chromatography, Gas / Q000379:methods; D008297:Male; D010665:Phenylpropanolamine / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D010136:p-Hydroxyamphetamine / Q000032:analysis; D010137:p-Hydroxynorephedrine / Q000032:analysis
D010665:Phenylpropanolamine; D010137:p-Hydroxynorephedrine; D000661:Amphetamine; D010136:p-Hydroxyamphetamine