Oxygraphic assay of 3,4-dihydroxyphenylalanine decarboxylase activity by coupled reaction with free and immobilized serum amine oxidase.
An oxygraphic method for the assay of 3,4-dihydroxyphenylalanine (Dopa) decarboxylase activity which makes use of the coupled reactions of Dopa decarboxylase with serum amine oxidase is presented. Both free and immobilized amine oxidases were utilized. The assay is simple, rapid, and allows a continuous monitoring of the reaction. The kinetic parameters for Dopa decarboxylase obtained with the coupled assay do not significantly differ from those obtained by standard methods.
D006631:Amine Oxidase (Copper-Containing); D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000378:metabolism*; D001921:Brain / Q000201:enzymology; D004296:Dopa Decarboxylase / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D004566:Electrodes; D004800:Enzymes, Immobilized; D007668:Kidney / Q000201:enzymology; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism*; D010100:Oxygen / Q000032:analysis*; D010101:Oxygen Consumption; D013552:Swine
D004800:Enzymes, Immobilized; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors; D004296:Dopa Decarboxylase; D001142:Aromatic-L-Amino-Acid Decarboxylases; D010100:Oxygen; D004298:Dopamine