[Isolation and properties of neurospecific proteins 14-3-2, 14-3-3 and 10-40-4].
A procedure for isolation of three neurospecific proteins 14-3-2, 14-3-3 and 10-40-4 from human brain is proposed. This procedure includes successive ammonium sulphate precipitation, ion-exchange chromatography on DEAE-cellulose, gel filtration through Sephadex G-150 and ion-exchange chromatography on DEAE-Sephadex A-50. Specific antisera raised against these antigens were used to prove their neurospecificity and to study their species-specificity. Some physico-chemical properties of the isolated proteins were investigated. The 14-3-2 protein--neurospecific enolase, appeared to be much more stable at high temperatures than the non-neuronal enzyme isoform.
D048948:14-3-3 Proteins; D000645:Ammonium Sulfate; D000818:Animals; D000941:Antigens / Q000276:immunology; D001921:Brain / Q000201:enzymology; D001923:Brain Chemistry; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology; D010751:Phosphopyruvate Hydratase / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology; D011817:Rabbits; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D000941:Antigens; D007106:Immune Sera; D009419:Nerve Tissue Proteins; C484014:neurospecific brain antigen 10-40-4, human; D014446:Tyrosine 3-Monooxygenase; D010751:Phosphopyruvate Hydratase; D000645:Ammonium Sulfate
Vydelenie i svoĭstva neĭrospetsificheskikh belkov 14-3-2, 14-3-3 i 10-40-4.