Functional groups on 'Z' DNA recognized by monoclonal antibodies.
Both brominated poly[d(GC)] and poly[d( Gm5C )] form stable left-handed Z-DNA structures at physiological ionic strengths. These two antigens were used to prepare monoclonal antibodies from immunized mice. The specificity of the antibodies was studied in detail with a solid-phase radioimmune assay as well as by means of competition experiments. Both immunogens produced several relatively non-specific antibodies but two types of very specific antibody were also distinguished. The first binds poly[d( Gm5C )] but not brominated poly[d(GC)] while the other has the opposite specificity and will only bind the brominated polymer.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D001667:Binding, Competitive; D001966:Bromine; D004247:DNA / Q000276:immunology*; D005260:Female; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009690:Nucleic Acid Conformation; D009994:Osmolar Concentration; D011089:Polydeoxyribonucleotides / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D011089:Polydeoxyribonucleotides; C019530:poly(dC-dG); C029988:poly(dG-m(5)dC).poly(dG-m(5)dC); D004247:DNA; D001966:Bromine