title: Leukokinesis in bovine ostertagiasis: stimulation of leukocyte migration by Ostertagia.
abstract: A blind-well chemotaxis chamber method was used to indicate migration stimulation of bovine neutrophil and eosinophil polymorphonuclear leukocytes and macrophages as related to ostertagiasis. Live exsheathed Ostertagia ostertagi 3rd-stage larvae (L3) and soluble L3 antigen (SLA), prepared by freeze thawing and sonic disruption of L3, enhanced cellular migration for eosinophils, but not for neutrophils and macrophages. Products of lymphocytes cultured with SLA for 3 to 6 hours were also examined, using lymphocytes from peripheral blood of helminth-free cattle and cattle infected with O ostertagi or Trichostrongylus axei. Lymphokines that enhanced cellular migration of neutrophils, eosinophils, and macrophages were present in culture supernatants of SLA-stimulated lymphocytes from O ostertagi-infected cattle, but not from cattle infected with T axei or helminth-free cattle. Seemingly, L3 and SLA were stimulants of eosinophil migration. Further, neutrophil, eosinophil, and macrophage migration was modulated by lymphokines produced by SLA-stimulated lymphocytes from cattle with ostertagiasis.
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000503:physiopathology*; D002634:Chemotaxis, Leukocyte; D004804:Eosinophils / Q000503:physiopathology; D008222:Lymphokines / Q000502:physiology; D008264:Macrophages / Q000503:physiopathology; D009504:Neutrophils / Q000503:physiopathology; D010029:Ostertagiasis / Q000503:physiopathology / Q000662:veterinary*; D014251:Trichostrongyloidea / Q000276:immunology; D014252:Trichostrongyloidiasis / Q000662:veterinary*
chemical_list: D008222:Lymphokines