Purification and properties of the membrane form of variant surface glycoproteins (VSGs) from Trypanosoma brucei.
Variant surface glycoproteins (VSG) of Trypanosoma brucei are released in a water soluble form on impairment of membrane integrity. We have previously shown that this release is the result of an enzyme-mediated event which converts the hydrophobic membrane form VSG into the hydrophilic water-soluble form. We now present further details of the methods by which membrane form VSG ( mfVSG ) may be isolated, uncontaminated by water-soluble VSG ( sVSG ). The sensitivity to different metal ions of the enzyme that mediated the conversion event is discussed, and some biochemical characteristics of different mfVSG preparations are presented.
D000535:Aluminum / Q000494:pharmacology; D002104:Cadmium / Q000494:pharmacology; D002846:Chromatography, Affinity; D006023:Glycoproteins / Q000302:isolation & purification* / Q000378:metabolism; D007457:Iodine Radioisotopes; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism; D012995:Solubility; D014346:Trypanosoma brucei brucei / Q000032:analysis* / Q000201:enzymology / Q000378:metabolism; D014643:Variant Surface Glycoproteins, Trypanosoma; D015032:Zinc / Q000494:pharmacology
D006023:Glycoproteins; D007457:Iodine Radioisotopes; D008565:Membrane Proteins; D014643:Variant Surface Glycoproteins, Trypanosoma; D002104:Cadmium; D000535:Aluminum; D015032:Zinc