title: Developmental changes in the composition of, and precursor incorporation into, polypeptides of rat brain slices.
abstract: Developmental changes in both polypeptide composition and incorporation of L-[3,4(n)-3H]valine into rat brain slice polypeptides have been monitored by SDS polyacrylamide gel electrophoresis coupled with fluorography. Six polypeptide bands (mol. wt 280, 210, 117, 66, 55 and 53 k) showed developmental decreases in relative amount whilst eleven others (mol. wt 230, 156, 95, 77, 68, 62, 48, 38, 34, 28 and 18 k) showed developmental increases. The majority of these changes were accompanied by corresponding, but not parallel changes in precursor incorporation. At least one polypeptide, mol. wt 44 k, showed a developmental decrease in precursor incorporation, but little change in relative amount. Localisation of these polypeptides in SPM, myelin and mitochondrial enriched fractions has been studied.
mesh_terms: D000367:Age Factors; D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000096:biosynthesis; D010452:Peptide Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D014633:Valine / Q000378:metabolism
chemical_list: D009419:Nerve Tissue Proteins; D014633:Valine