[Analysis of cytoplasmic aspartate aminotransferase using active enzyme centrifugation].
Using active enzyme centrifugation, it was demonstrated that pig heart cytoplasmic aspartate aminotransferase retains its dimeric conformation with a decrease in protein concentration down to 0.2 microgram/ml (50 mM L-aspartate, 5 mM alpha-ketoglutarate, 0.1 M NaCl, 0.05 M potassium-phosphate buffer, pH 7.4, 20 degrees C).
D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis*; D003593:Cytoplasm / Q000201:enzymology; D004789:Enzyme Activation; D046911:Macromolecular Substances; D009206:Myocardium / Q000201:enzymology*; D013552:Swine; D014461:Ultracentrifugation
D046911:Macromolecular Substances; D001219:Aspartate Aminotransferases
Izuchenie tsitoplazmaticheskoĭ aspartataminotransferazy metodom sedimentatsii aktivnogo fermenta.