Quantitative analysis of hyaluronic acid by high-performance liquid chromatography of streptomyces hyaluronidase digests.
The separation and quantitative analysis of streptomyces hyaluronidase ( HAase ) degradation products of hyaluronic acid (HA) by high-performance liquid chromatography are described. The substituted 4,5-unsaturated tetrasaccharide and hexasaccharide which result from the digestion of HA are quickly separated on a silica gel (Zorbax SIL) column. The assay of HA is linear between 5 and 250 micrograms of HA. This procedure is suitable for some weakly acidic glycosaminoglycan contaminants having similar properties to those of HA.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006820:Hyaluronic Acid / Q000032:analysis*; D006821:Hyaluronoglucosaminidase / Q000378:metabolism*; D007700:Kinetics; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013302:Streptomyces / Q000201:enzymology*
D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase