Determination of 1-methylimidazole-4-acetic acid in brain tissue from rat and mouse.
The concentration of the histamine metabolite 1-methylimidazole-4-acetic acid was determined in brain tissue from rat and mouse with a gas chromatographic-mass spectrometric method. Mouse brain contained 1.7-3.2 nmol/g, depending on the strain. The concentration in cerebrum from Sprague-Dawley rats was 1.2 nmol/g, whereas cerebellum contained 0.24 nmol/g. The concentration of tele-methylhistamine in mouse brain was 1.4-2.2 nmol/g. The concentration of 1-methylimidazole-4-acetic acid in rat brain after death did not change significantly during 2 h at room temperature.
D000818:Animals; D001923:Brain Chemistry; D002531:Cerebellum / Q000032:analysis; D006719:Homovanillic Acid / Q000032:analysis; D007093:Imidazoles / Q000032:analysis*; D008297:Male; D013058:Mass Spectrometry; D008761:Methylhistamines / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D007093:Imidazoles; D008761:Methylhistamines; C002667:methylimidazoleacetic acid; D006719:Homovanillic Acid