Quantitative uptake studies of 131I-labeled (E)-5-(2-iodovinyl)-2'-deoxyuridine in herpes simplex virus-infected cells in vitro.
We have synthesized a 131I-radiolabeled antiviral compound (E)-5-(2-iodovinyl)-2'-deoxyuridine (IVdU) and shown that this agent was selectively trapped within rabbit kidney cells, infected in vitro by thymidine kinase-positive (TK+) herpes simplex virus (HSV). The uptake of 131I-labeled IVdU was specific, as it was not concentrated within either HSV (TK-) or mock-infected cells. In certain conditions, over 40% of the radiolabel was selectively trapped within HSV (TK+)-infected cells. This was a 20- to 30-fold increase over the uptake of 131I-labeled IVdU by HSV (TK-) or mock-infected cells. The uptake of 131I-labeled IVdU varied directly with (i) the dose of the virus used to infect the rabbit kidney cells; (ii) the concentration of radiolabeled IVdU added to the system; and (iii) the time of exposure of IVdU to infected cells. The ability of this agent to be trapped within HSV (TK+)-infected cells merits further evaluation in animal models as it has potential as a noninvasive, herpes-specific diagnostic test, in particular for HSV encephalitis.
D000818:Animals; D003114:Colony-Forming Units Assay; D006561:Herpes Simplex / Q000378:metabolism*; D007065:Idoxuridine / Q000031:analogs & derivatives* / Q000378:metabolism; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D007668:Kidney; D011817:Rabbits; D013937:Thymidine Kinase / Q000378:metabolism; D013997:Time Factors; D014776:Virus Cultivation
D007457:Iodine Radioisotopes; C030360:5-(2-iodovinyl)-2'-deoxyuridine; D013937:Thymidine Kinase; D007065:Idoxuridine