title: A new and improved gas chromatographic method for the determination of fluoride in plasma and faeces.
abstract: A method is described that extracts fluoride selectively as trimethylfluorosilane using carbon tetrachloride and trimethylchlorosilane . Drawbacks of other methods as well as advantages of the proposed method are discussed. The method is directly applicable to plasma and faeces samples, has a short gas chromatographic run time due to the use of a backflush technique and is suitable for automation. Its lowest quantifiable limit for plasma and faeces is 10 micrograms/l and 1 microgram/g fluoride respectively, using 1.0 ml or 1.0 g samples. Preliminary pharmacokinetic parameters of fluoride obtained in two subjects are reported.
mesh_terms: D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D004340:Drug Contamination; D005243:Feces / Q000032:analysis*; D005459:Fluorides / Q000032:analysis* / Q000097:blood; D007700:Kinetics
chemical_list: D005459:Fluorides