Suppression of bronchoalveolar lymphocyte antibody secretion by alveolar macrophage: an in vitro study in the rabbit.
The effect of rabbit alveolar macrophage (AM) on the antibody secretion of bronchoalveolar lymphocytes was investigated in vitro, using a plaque-forming cell assay. Animals were intratracheally primed and reimmunized with 10(10) sheep red blood cells (SRBC). Free alveolar cells were obtained by lung lavage and broncho-alveolar lymphocyte-enrichment was achieved through Sephadex G-10 columns. Cell suspensions with various macrophage-to-lymphocyte ratios (AM:L from 1:25 to 3:1) were prepared using appropriate numbers of plastic adherent alveolar cells. After a 4 day in vitro co-culture of 5 X 10(6) alveolar cells (AM plus lymphocytes) with 3 X 10(6) SRBC, IgM and IgG antibody-forming cells (AFC) were counted. A significant suppressive effect (p less than 0.05) of AM on both IgM and IgG-AFC was observed when AM:L ratio was increased from 1:25 to 1:10. This effect was slightly accentuated with higher AM:L values, required viable AM, and was not affected by adding indomethacin in the culture medium. Moreover, a preliminary co-culture of AM with bronchoalveolar lymphocytes and the antigen was necessary for the expression of this suppressive effect. These data suggest that the suppressive activity of primed AM on alveolar lymphocytes may be of physiologic significance in vivo in the regulation of one of the pulmonary immune responses to airborne antigens, namely local antibody production.
D000818:Animals; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D007075:Immunoglobulin M / Q000032:analysis; D007213:Indomethacin / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008264:Macrophages / Q000166:cytology / Q000502:physiology*; D008297:Male; D011650:Pulmonary Alveoli / Q000166:cytology*; D011817:Rabbits
D007075:Immunoglobulin M; D007213:Indomethacin