Determination of 17 beta-hydroxy-1 alpha-methyl-17 alpha-propyl-5-alpha-androstan-3-one in plasma by gas chromatography-mass spectrometry with single-ion detection.
The topical anti-androgen 17 beta-hydroxy-1 alpha-methyl-17 alpha-propyl-5 alpha-androstan-3-one is determined in plasma samples by extracting with ether and subsequent mass fragmentography with single-ion detection at m/z 303. 17 beta-Hydroxy-1 alpha-methyl-17 alpha-pentyl-5 alpha-androstan-3-one, added to the samples before extraction, is used as the internal standard. Reproducibility was calculated to be +/- 5.9% at the 5-ng/mL level and 0.4% at the 20-ng/mL level. The limit of detection is approximately 1 ng/mL. Total gas chromatography-mass spectrometry analysis time is approximately 10 min/sample.
D000287:Administration, Topical; D000328:Adult; D000726:Androgen Antagonists / Q000097:blood*; D013196:Dihydrotestosterone / Q000031:analogs & derivatives*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D007700:Kinetics; D008297:Male; D008655:Mesterolone / Q000031:analogs & derivatives* / Q000097:blood; D013696:Temperature
D000726:Androgen Antagonists; D013196:Dihydrotestosterone; D008655:Mesterolone; C041614:17-propylmesterolone