Effect of inoculum, pH, and medium on the activity of ciprofloxacin against anaerobic bacteria.
The in vitro activity of ciprofloxacin against 30 Bacteroides fragilis group, 30 Clostridium, and 30 Peptococcaceae strains was determined by the agar dilution method in two different culture media at three pH and in three inoculum densities. In Wilkins-Chalgren agar the MICs for 90% of the strains were 0.12 micrograms/ml for B. fragilis, 0.5 micrograms/ml for Clostridium spp., and 2 micrograms/ml for Peptococcaceae. The pH and medium composition affected the MICs of ciprofloxacin.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001421:Bacteria, Anaerobic / Q000187:drug effects*; D001441:Bacteroides fragilis / Q000187:drug effects; D002939:Ciprofloxacin; D003013:Clostridium / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008795:Metronidazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010459:Peptococcaceae / Q000187:drug effects; D011804:Quinolines / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D003470:Culture Media; D011804:Quinolines; D008795:Metronidazole; D002939:Ciprofloxacin