title: Quantitative capillary column gas chromatographic method for the determination of glycopyrronium in human plasma.
abstract: A new sensitive and selective capillary column gas chromatographic method for the anti-cholinergic agent glycopyrronium bromide in human plasma is described. The procedure involves preliminary ion-pair extraction of the drug into dichloromethane, followed by concentration and analysis of the ion-pair complex by capillary column gas chromatography using a nitrogen-sensitive detector. The method depends on the thermal dequaternisation of the quaternary ammonium compound and can be used to detect 5 ng/ml in a 3-ml plasma sample. The assay procedure has been applied to the determination of the plasma concentration of glycopyrronium after intravenous administration to an anaesthetised patient.
mesh_terms: D001561:Benzilates / Q000097:blood; D002849:Chromatography, Gas / Q000379:methods; D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006024:Glycopyrrolate / Q000097:blood*; D006801:Humans; D010880:Piperidines / Q000097:blood; D011759:Pyrrolidines / Q000097:blood*
chemical_list: D001561:Benzilates; D010880:Piperidines; D011759:Pyrrolidines; C005101:mepenzolic acid; D006024:Glycopyrrolate