Elemental X-ray microanalysis of mouse peritoneal and bone marrow derived cultured mast cells.
Elemental X-ray microanalysis of intact mouse peritoneal cells revealed significantly high K alpha sulfur and chloride peaks, while phosphorus, sodium and calcium displayed relatively low K alpha peaks. Bone marrow derived cultured mast cells exhibited a low K alpha peak for sulfur and a high K alpha peak for chloride. Peritoneal macrophages which served as controls differed from peritoneal mast cells by their high phosphorus and chloride content and a relatively low sulfur peak. The finding of two different spectra for the mast cells may provide additional parameters for discrimination of mast cell subsets.
D000818:Animals; D001854:Bone Marrow Cells; D002118:Calcium / Q000032:analysis; D002478:Cells, Cultured; D004577:Electron Probe Microanalysis; D008407:Mast Cells / Q000000981:diagnostic imaging* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D010537:Peritoneum / Q000166:cytology; D010758:Phosphorus / Q000032:analysis; D011859:Radiography; D012964:Sodium / Q000032:analysis; D013455:Sulfur / Q000032:analysis
D010758:Phosphorus; D013455:Sulfur; D012964:Sodium; D002118:Calcium