N-acetyl-beta-D-glucosaminidase (NAGase) levels in bulk herd milk.
N-acetyl-beta-D-glucosaminidase ( NAGase ) levels and somatic cell counts (SCC) were determined monthly for 6 months in the bulk milk of 181 suppliers (1063 samples). A highly significant correlation (r = 0.74; P less than 0.001) was found between supplier's bulk herd milk geometric mean NAGase activity and SCC. Monthly trends which grouped suppliers into various categories defined by different NAGase and SCC thresholds showed that a similar overall pattern was obtained with both SCC and NAGase . However, further analysis indicated that 18% of the bulk milk which had SCC less than 500 X 10(3)/ml had NAGase levels greater than 25 units. Also, 34% of samples with SCC greater than 500 X 10(3)/ml had NAGase levels less than 25 units. Overall, 24% of all samples did not have corresponding NAGase and SCC results. When the bulk milk of 2 commercial dairy herds was tested monthly over 12-18 months whilst the infection status of all quarters in the herds was regularly monitored, those herds with low incidence of mastitis (5% quarters infected) had significantly lower bulk milk SCC and NAGase levels than those with a high incidence of mastitis (22% of quarters infected). This suggests that NAGase measurement on bulk herd milk would be a simple means of monitoring infection status of dairy herds and of rapidly classifying a supplier's milk as being of low, medium or high SCC status. The possible combined use of SCC and NAGase levels in bulk milk monitoring schemes is discussed.
D000118:Acetylglucosaminidase / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002452:Cell Count / Q000662:veterinary; D005260:Female; D006596:Hexosaminidases / Q000378:metabolism*; D008414:Mastitis, Bovine / Q000175:diagnosis / Q000201:enzymology*; D008892:Milk / Q000166:cytology / Q000201:enzymology*
D006596:Hexosaminidases; D000118:Acetylglucosaminidase