Copper-phenanthroline-induced site-specific oxygen-radical damage to DNA. Detection of loosely bound trace copper in biological fluids.
Copper(II) ions, in the presence of 1,10-phenanthroline, O2 and a reducing agent, degrade DNA with the release of thiobarbituric-acid-reactive material. This reaction, dependent on the formation of oxygen radicals, was made the basis of a sensitive and specific assay for loosely bound copper in body fluids. When applied to certain extracellular fluids, trace amounts of copper could be detected in the lower micromolar range.
D001665:Binding Sites; D001826:Body Fluids / Q000032:analysis*; D003300:Copper / Q000032:analysis*; D004247:DNA; D005609:Free Radicals; D006801:Humans; D010100:Oxygen; D010618:Phenanthrolines; D013050:Spectrometry, Fluorescence
D005609:Free Radicals; D010618:Phenanthrolines; D003300:Copper; D004247:DNA; D010100:Oxygen; C025205:1,10-phenanthroline