Analysis of T antigen and viral DNA in mouse cells transformed by K virus, a nononcogenic murine papovavirus.
A tumorigenic line of mouse cells transformed by the nononcogenic murine K papovavirus was established and characterized. Tumor-bearing animals produced antibody specific for K virus-transformed cells; the K virus T antiserum did not react with polyomavirus-transformed cells. Immunoprecipitation of the T antigen in the transformed cells revealed large T antigen (molecular weight 84,000). About 10 to 15 copies of K virus DNA were found to be integrated in the cellular DNA.
D000818:Animals; D000831:Animals, Newborn; D000957:Antigens, Viral, Tumor / Q000032:analysis*; D002460:Cell Line; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D008168:Lung / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D027383:Papillomaviridae / Q000235:genetics* / Q000276:immunology; D027382:Polyomaviridae
D000957:Antigens, Viral, Tumor; D004279:DNA, Viral