title: Role of dietary iron in maturation of rat small intestine at weaning.
abstract: The weanling process is characterized by the transition from a liquid diet poor in iron (rat milk) to a solid diet high in iron (chow pellets). To examine the effects of iron content of the weanling diet on terminal maturation of rat small intestine, suckling pups, nursed by iron-sufficient mothers, were weaned by day 16 onto a solid basal diet that was either deficient [low-iron diet (LID): 0.5 mg iron/100 g solid] or high [high-iron diet (HID) controls: 30 mg iron/100 g solid] in iron. The animals were studied during or at the end of the 4th postnatal wk. By day 17 rats weaned onto the LID exhibited an initial rise in jejunal sucrase activity as did their controls, but the activity plateau of the enzyme was reduced to a level 60% of the controls. On day 28 iron-deprived rats were anemic and showed significant decreases (P less than 0.01 compared with HID rats) in the activity of jejunal sucrase (-57%), neutral lactase (-83%), and maltase (-46%), whereas villus height, crypt depth, mucosal mass parameters, ileal acid beta-galactosidase activity, mucosal protein, and DNA synthesis rates were equivalent in LID and HID groups. The concentration of the secretory component, a glycoprotein synthesized by the intestinal crypt cell, was markedly depressed (P less than 0.01 vs. controls) in the jejunum (-54%) and ileum (-79%) of iron-deprived rats. When D-[1-14C]glucosamine was injected intraperitoneally, incorporation of the label into jejunal and ileal brush-border proteins was two to three times lower for iron-deficient rats than for controls.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D004032:Diet; D006023:Glycoproteins / Q000096:biosynthesis; D007413:Intestinal Mucosa / Q000032:analysis; D007421:Intestine, Small / Q000254:growth & development*; D007501:Iron / Q000494:pharmacology*; D000090463:Iron Deficiencies; D007583:Jejunum / Q000201:enzymology; D008871:Microvilli / Q000378:metabolism; D051381:Rats / Q000254:growth & development*; D011919:Rats, Inbred Strains; D012635:Secretory Component / Q000032:analysis; D013393:Sucrase / Q000378:metabolism; D014886:Weaning
chemical_list: D006023:Glycoproteins; D012635:Secretory Component; D007501:Iron; D013393:Sucrase