Escherichia coli glutaminyl-tRNA synthetase. II. Characterization of the glnS gene product.
Glutaminyl-tRNA synthetase has been purified by a simple, two-column procedure from an Escherichia coli K12 strain carrying the glnS structural gene on plasmid pBR322. The primary sequence of this enzyme as derived from the DNA sequence (see accompanying paper) has been confirmed. Manual Edman degradation was used to identify the NH2-terminal sequence of the protein. Oligopeptides scattered throughout the primary sequence of glutaminyl-tRNA synthetase were sequenced by the gas chromatographic-mass spectrometric method and matched to the theoretical peptides derived from the translated DNA sequence. The expected carboxyl terminus at position 550 was verified by carboxypeptidase B digestion. The primary sequence of glutaminyl-tRNA synthetase contains no extensive sequence repeats. A search was made for sequence homologies between this enzyme and the few other aminoacyl-tRNA synthetases for which primary sequences are available. A single homologous region is shared by at least three of the synthetases examined here.
D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D019286:Endopeptidase K; D010450:Endopeptidases; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D008401:Gas Chromatography-Mass Spectrometry; D005796:Genes; D010446:Peptide Fragments / Q000032:analysis; D013820:Thermolysin
D004269:DNA, Bacterial; D010446:Peptide Fragments; D010450:Endopeptidases; D019286:Endopeptidase K; D013820:Thermolysin; D000604:Amino Acyl-tRNA Synthetases; C020659:glutaminyl-tRNA synthetase