Beta-hydroxyaspartic acid in vitamin K-dependent plasma proteins from scorbutic and warfarin-treated guinea pigs.
beta- Hydroxyaspartic acid is a rare amino acid, present in all vitamin K-dependent plasma proteins except prothrombin, and is formed by a post-translational hydroxylation of aspartic acid. We have now investigated whether this hydroxylation, like that of proline in collagen, is vitamin C-dependent. The vitamin K-dependent plasma proteins were isolated from normal and scorbutic guinea pig plasma by barium citrate adsorption and the beta- hydroxyaspartic acid content was determined. Compared with normal animals, scorbutic animals showed no significant reduction of beta- hydroxyaspartic acid content. In warfarin-treated animals there was a decreased content of both beta- hydroxyaspartic acid and gamma-carboxyglutamic acid in the barium citrate adsorbed fraction. It was concluded that the post-translational hydroxylation of aspartic acid is unlikely to be vitamin C-dependent.
D015055:1-Carboxyglutamic Acid / Q000097:blood; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001206:Ascorbic Acid Deficiency / Q000097:blood*; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000097:blood; D001777:Blood Coagulation; D001798:Blood Proteins / Q000378:metabolism*; D006168:Guinea Pigs; D006900:Hydroxylation; D011499:Protein Processing, Post-Translational; D014812:Vitamin K / Q000494:pharmacology*; D014859:Warfarin / Q000494:pharmacology*
D001798:Blood Proteins; D014812:Vitamin K; C011274:3-hydroxyaspartic acid; D001224:Aspartic Acid; D015055:1-Carboxyglutamic Acid; D014859:Warfarin; D001205:Ascorbic Acid