Factors influencing renal cryopreservation. I. Effects of three vehicle solutions and the permeation kinetics of three cryoprotectants assessed with rabbit cortical slices.
A renal cortical slice model was used to assess the effects on viability of three vehicle solutions-Krebs-Henseleit (K-H), solution A, and RPS-2--at 25 degrees C. After 120 min incubation no differences in [K+]/[Na+] ratios were found. Tracer techniques were used to study the osmotic effects and permeation kinetics at 25 degrees C of three cryoprotectants (dimethyl sulfoxide (Me2SO), ethylene glycol, and glycerol) and the effect of the vehicle solution (K-H or RPS-2) on Me2SO kinetics. It was found that Me2SO was most permeable and ethylene glycol least, and that ethylene glycol had unusual effects which suggest that it may not act as a simple solute. Differences were found when Me2SO was introduced in K-H and RPS-2 that are believed to be related to the binding properties of Me2SO to cell constituents.
D000818:Animals; D002463:Cell Membrane Permeability; D002470:Cell Survival; D003451:Cryoprotective Agents / Q000008:administration & dosage*; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage; D019855:Ethylene Glycol; D005026:Ethylene Glycols / Q000008:administration & dosage; D005260:Female; D005615:Freezing; D005990:Glycerol / Q000008:administration & dosage; D007668:Kidney; D007672:Kidney Cortex; D007700:Kinetics; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D009995:Osmosis; D011817:Rabbits; D012996:Solutions; D013997:Time Factors
D003451:Cryoprotective Agents; D005026:Ethylene Glycols; D012996:Solutions; D019855:Ethylene Glycol; D005990:Glycerol; D004121:Dimethyl Sulfoxide