Determination of nafazatrom in body fluids by high-performance thin-layer chromatography with post-chromatographic derivatization.
A sensitive, accurate and specific high-performance thin-layer chromatographic method has been developed for determination of nafazatrom, a pyrazolinone derivative with antithrombotic and antimetastatic activity. Because of the instability of the drug during clean-up procedures, nafazatrom is recovered from plasma and urine with a single extraction and the extract is spotted directly onto the chromatographic plate. The specificity of the assay was increased by post-chromatographic derivatization with 4-dimethylaminobenzaldehyde. Up to eight primary metabolites with an unchanged position 4 in the heterocyclic ring were also detected and separated chromatographically. The detection limit for nafazatrom is 0.5 ng per spot and 5 ng/ml. The analytical error in the nanogram per millilitre range is less than 10%, low enough for pharmacokinetic studies and for plasma level monitoring in patients.
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer / Q000379:methods; D006801:Humans; D007700:Kinetics; D011720:Pyrazoles / Q000032:analysis* / Q000097:blood / Q000652:urine; D047069:Pyrazolones; D011786:Quality Control
D011720:Pyrazoles; D047069:Pyrazolones; C018845:nafazatrom