title: Characterization of the ATP-G-actin aggregates formed at low potassium chloride concentration.
abstract: The ATP-G-actin aggregates formed by incubation of ATP-G-actin in 7.5 mM-KCl were characterized by electron-microscopical observation, by high-pressure liquid chromatography and by the study of the 1,N6-etheno-ATP-ATP exchange reaction between the free and the actin-bound nucleotide. In 30 mM-KCl the initial rate of the reduced-viscosity increase is found to be directly related to the amount of the aggregates formed in the course of the preincubation in 7.5 mM-KCl.
mesh_terms: D000199:Actins; D000255:Adenosine Triphosphate; D002851:Chromatography, High Pressure Liquid; D004985:Ethenoadenosine Triphosphate; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D011189:Potassium Chloride; D011485:Protein Binding
chemical_list: D000199:Actins; D004985:Ethenoadenosine Triphosphate; D046911:Macromolecular Substances; D011189:Potassium Chloride; D000255:Adenosine Triphosphate