title: An ultrastructural analysis of osteochondritic growth plate cartilage in growing swine.
abstract: In growing swine, ossification failure due to osteochondrosis has an incidence of nearly 100% in the distal ulna of animals at six months of age, yet the etiology of the disease is understood poorly. In this study, the ultrastructure of the chondrocyte and its pericellular matrix is analyzed in normal growth plates and in growth plates with lesions characteristic of osteochondrosis using aldehyde primary fixatives and osmium-ferrocyanide as the secondary fixative. Chondrocytes in lesion areas fail to undergo normal hypertrophic cell maturation, and they have an accumulation of rough endoplasmic reticulum, lipid droplets and mitochondria. These morphological changes are interpreted to be both variable and nonspecific for osteochondrosis. Within the pericellular matrix of chondrocytes from lesion areas, the most striking abnormality is the presence of a highly condensed matrix with an accumulation of large, irregularly shaped deposits of electron dense material. These morphological alterations are characteristic of a matrix which either is not secreted normally, or in which the highly ordered interactions of diverse macromolecules has been lost. These pericellular matrix changes have not been described in other diseases of growing cartilage. They may be significant in the failure of metaphyseal vascular penetration of the pericellular matrix which is characteristic of osteochondrosis.
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000473:pathology / Q000648:ultrastructure; D006132:Growth Plate / Q000254:growth & development / Q000473:pathology / Q000648:ultrastructure*; D006984:Hypertrophy / Q000662:veterinary; D008297:Male; D010007:Osteochondritis / Q000473:pathology / Q000503:physiopathology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000473:pathology* / Q000503:physiopathology; D013556:Swine, Miniature; D014457:Ulna