Analysis of the interaction between DNA and major core protein in adenovirus chromatin by circular dichroism and ultraviolet light induced cross-linking.
Adenovirus chromatin is constituted with three kinds of core proteins, VII, V, and mu, that are coded by the virus genome. Since a hexamer of VII contributes to formation of the nucleosome-like structure of the virion chromatin, we analyzed the interaction between DNA and VII in vitro, by the use of ultraviolet light-induced cross-linking and circular dichroism (CD) spectroscopy. It was observed that DNA and VII in a plain mixture form a structure resembling viral chromatin. The DNA in the virion core or in the simply mixed complex appears to take a tight conformation by superfolding, based on the result that the ellipticity at 275 nm of DNA was reduced to approximately 3,000 degrees, and the wave-length of the positive peak was shifted from 275 to 285 nm. The change in CD spectrum caused by interaction of VII with DNA is similar to that of a protamine rather than that of a histone mixture. The interaction of VII with DNA is preferential, and VII is capable of associating more efficiently with double stranded DNA than with single stranded. The interaction is loosened by salt (0.3 M NaCl) and tightened by magnesium ion. However, the interaction of a precursor core protein pro-VII with DNA was not as tight as that of VII and was not influenced by magnesium ion, presumably because of the existence of a hydrophobic processing sequence in the molecule.
D000260:Adenoviruses, Human / Q000235:genetics*; D002277:Carcinoma; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D002942:Circular Dichroism; D004279:DNA, Viral / Q000378:metabolism* / Q000528:radiation effects; D003852:Deoxyribonucleoproteins / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009062:Mouth Neoplasms; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D014466:Ultraviolet Rays; D014758:Viral Core Proteins; D014764:Viral Proteins / Q000378:metabolism* / Q000528:radiation effects
D002843:Chromatin; D004279:DNA, Viral; D003852:Deoxyribonucleoproteins; D014758:Viral Core Proteins; D014764:Viral Proteins