Dissolution profile determination of a multicomponent product using a rapid liquid chromatographic analysis.
A system was developed which is capable of monitoring the dissolution profiles of each of the active components in a two-component combination product. The UV spectra of the two drugs (metoprolol and hydrochlorothiazide) overlap considerably, making conventional UV analysis of either component unrealistic. By resolving the two drugs on a high-performance liquid chromatographic (HPLC) system both can be quantitated after separation. The analysis is sufficiently short to allow for the use of a six-station dissolution tester in tandem with an HPLC for determination of dissolution profiles. The HPLC system and equipment, can monitor six samples of this product at 5-min intervals in real analysis time.
D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004338:Drug Combinations; D006852:Hydrochlorothiazide / Q000032:analysis; D008790:Metoprolol / Q000032:analysis; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D004338:Drug Combinations; D006852:Hydrochlorothiazide; D008790:Metoprolol