Evidence that intracellular synthesis of 5-fluorouridine-5'-phosphate from 5-fluorouracil and 5-fluorouridine is compartmentalized.
L1210 cells were exposed to equitoxic concentrations of [14C]5-fluorouracil and [3H]5-fluorouridine for 4 hours. The RNA from these cells was separated into cytosolic and nuclear fractions, and then further fractionated by chromatography on poly-U Sepharose, Sephadex G-200 and DEAE-cellulose. The ratio of tritium to carbon-14 incorporated into various species of RNA differed by as much as 6-fold, indicating that the respective 5-fluorouridine-5'-monophosphates synthesized from the two precursors are localized in separate pools that do not mix rapidly.
D000818:Animals; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000378:metabolism; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003600:Cytosol / Q000378:metabolism; D005472:Fluorouracil / Q000378:metabolism*; D007939:Leukemia L1210 / Q000378:metabolism; D051379:Mice; D012334:RNA, Neoplasm / Q000302:isolation & purification; D012685:Sepharose / Q000031:analogs & derivatives; D014500:Uracil Nucleotides / Q000096:biosynthesis*; D014529:Uridine / Q000031:analogs & derivatives* / Q000378:metabolism
D012334:RNA, Neoplasm; D014500:Uracil Nucleotides; C030188:poly U-sepharose; C001943:5-fluorouridine; D012685:Sepharose; C016462:5-fluorouridine 5'-phosphate; D005472:Fluorouracil; D014529:Uridine