Purification of S-acyl fatty acid synthase thioester hydrolase by affinity chromatography with fatty acid synthase attached to Sepharose.
S-Acyl fatty acid synthase thioester hydrolase, the enzyme responsible for release of short-chain fatty acids from fatty acid synthase, was purified to homogeneity in 50-60% yield by a procedure involving affinity chromatography with fatty acid synthase as the affinity ligand attached to Sepharose. This procedure gave a 10- to 12-fold increase in yield over the previously available method.
D000818:Animals; D002846:Chromatography, Affinity; D004372:Ducks; D004800:Enzymes, Immobilized; D064429:Fatty Acid Synthases; D005777:Geese; D012627:Sebaceous Glands / Q000201:enzymology; D012685:Sepharose; D013869:Thiolester Hydrolases / Q000302:isolation & purification*
D004800:Enzymes, Immobilized; D012685:Sepharose; D064429:Fatty Acid Synthases; D013869:Thiolester Hydrolases; C018748:oleoyl-(acyl-carrier-protein) hydrolase