Chemical modification of Cys-374 of actin interferes with the formation of the profilactin complex.
Chemical modification of the cysteine residue 374 of actin, both with N-ethylmaleimide and with the fluorescent probe N-(1-pyrenyl)iodoacetamide, is shown to counteract the inhibiting effect of profilin on actin polymerization.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D003285:Contractile Proteins / Q000378:metabolism; D003545:Cysteine; D005033:Ethylmaleimide / Q000494:pharmacology*; D005456:Fluorescent Dyes / Q000494:pharmacology*; D007460:Iodoacetamide / Q000031:analogs & derivatives* / Q000494:pharmacology; D007461:Iodoacetates; D046911:Macromolecular Substances; D008840:Microfilament Proteins; D051304:Profilins; D011506:Proteins / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013154:Spleen / Q000378:metabolism
D000199:Actins; D003285:Contractile Proteins; D005456:Fluorescent Dyes; D007461:Iodoacetates; D046911:Macromolecular Substances; D008840:Microfilament Proteins; D051304:Profilins; D011506:Proteins; C024782:profilactin; C029358:N-(1-pyrenyl)iodoacetamide; D003545:Cysteine; D005033:Ethylmaleimide; D007460:Iodoacetamide