title: A new platelet aggregating material (PAM) in an experimentally induced rat fibrosarcoma.
abstract: The present work concerns our studies to search for factor(s) which may influence the hemostatic process in or around metastasis of tumours. We studied the platelet aggregating property of a methyl cholanthrene induced experimental tumour. Platelet aggregating material was found to be different from the known aggregating agents like thrombin, ADP, collagen, thromboxane A2 and trypsin. It depends on a critical level of calcium for its action. PAM is a high molecular weight substance which contains sialic acid. It is trypsin and plasmin insensitive. The activity of this substance is not being destroyed by phospholipase-C. Metabolic study indicates that PAM acts by mitochondrial energy metabolic pathway of the platelets.
mesh_terms: D000818:Animals; D001779:Blood Coagulation Factors / Q000032:analysis*; D002118:Calcium / Q000378:metabolism; D005354:Fibrosarcoma / Q000032:analysis* / Q000139:chemically induced / Q000556:secondary; D008297:Male; D008748:Methylcholanthrene / Q000009:adverse effects; D008970:Molecular Weight; D009368:Neoplasm Transplantation; D010972:Platelet Activating Factor; D010974:Platelet Aggregation; D051381:Rats; D012983:Soft Tissue Neoplasms / Q000032:analysis* / Q000139:chemically induced / Q000556:secondary
chemical_list: D001779:Blood Coagulation Factors; D010972:Platelet Activating Factor; D008748:Methylcholanthrene; D002118:Calcium