High-performance liquid chromatographic determination of diltiazem and six of its metabolites in human urine.
The use of the calcium antagonist diltiazem (I) is increasing, particularly in treating unstable angina. This study describes a simple and specific high-performance liquid chromatographic method for the determination of I and six of its metabolites in urine. Diltiazem and its conjugated and unconjugated metabolites were assayed in the urine of patients treated with a dose of 120 mg po of diltiazem tid. Sensitivity of the method was 100 ng/mL for I and the metabolites desacetyldiltiazem (II), N-desmethyldesacetyldiltiazem (IV), O-desmethyldesacetyldiltiazem (V), O-desmethyl-N-desmethyldesacetyldiltiazem (VII), N-oxide desacetyldiltiazem (III), and O-desmethyl-N-oxide desacetyldiltiazem (VI). In the urine of patients, the extent of conjugation of six metabolites was greater than 80%.
D001552:Benzazepines / Q000652:urine*; D001711:Biotransformation; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004110:Diltiazem / Q000652:urine*; D006801:Humans; D007700:Kinetics
D001552:Benzazepines; D004110:Diltiazem