Separation of sialyl-oligosaccharides by high-performance liquid chromatography. Application to analysis of carbohydrate units of acidic oligosaccharides obtained by hydrazinolysis of hen ovomucoid.
Sialyl- oligossacharides derived from hen ovomucoid by hydrazinolysis have been separated by liquid chromatography on quaternary amine bonded silica and alkylamine modified silicas . By using a mobile phase consisting of a mixture of acetonitrile and potassium dihydrogen phosphate with 0.01% of 1,4- diaminobutane , effective resolution of high-molecular-weight monosialylated oligossacharides was achieved in less than 2 h.
D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004527:Egg Proteins; D005260:Female; D007202:Indicators and Reagents; D009844:Oligosaccharides / Q000302:isolation & purification*; D010059:Ovomucin; D013329:Structure-Activity Relationship
D002241:Carbohydrates; D004527:Egg Proteins; D007202:Indicators and Reagents; D009844:Oligosaccharides; C016735:sialooligosaccharides; D010059:Ovomucin