title: Fasciola hepatica: tegumental alterations as a consequence of lectin binding.
abstract: Adult flukes, Fasciola hepatica, incubated in Hedon - Fleig saline containing concanavalin A (Con A) for 10 and 45 min, respectively, exhibited severe alterations to tegumental morphology involving increased secretory activity, blebbing of the apical plasma membrane, increased total surface area, and swelling of the basal infolds . The effects of Con A were prevented by the addition of alpha-methyl-D-mannoside to the incubating medium. Similar, but less pronounced, effects were caused by wheat germ agglutinin (WGA) binding. Con A and WGA binding indicate the presence of mannose, glucosamine, or glucose moieties and of N-acetylglucosamine. The effects of lectin binding were similar to the early effects of antibody attachment, and it was considered that accelerated membrane turnover was occurring in both cases. Swelling of the basal infolds was thought to be a result of increased apical surface membrane and/or increased permeability due to lectin binding.
mesh_terms: D003208:Concanavalin A / Q000494:pharmacology; D005210:Fasciola hepatica / Q000187:drug effects / Q000648:ultrastructure*; D037102:Lectins / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D012550:Schistosoma mansoni / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D037102:Lectins; D003208:Concanavalin A