Improved focus assay of reticuloendotheliosis virus in a quail fibroblast cell line (QT35).
Reticuloendotheliosis virus (REV) strains T and CS consistently induced focus formation in a quail fibroblast cell line designated QT35 that was maintained under agarose overlay following inoculation of REV. The foci were more distinct and better circumscribed than those that developed in QT35 cultures under fluid medium. The focus formation in QT35 cultures under agarose overlay was inhibited by REV antiserum, and there were a highly significant correlation (r = 0.966, P less than 0.005) and a linear relationship between the number of foci that developed and relative virus concentration inoculated. This provides a valid focus assay for REVs.
D000818:Animals; D002460:Cell Line; D002645:Chickens; D003370:Coturnix; D003470:Culture Media; D005260:Female; D005347:Fibroblasts; D008297:Male; D011201:Poultry Diseases / Q000382:microbiology; D055761:Reticuloendotheliosis, Avian / Q000382:microbiology*; D012190:Retroviridae / Q000254:growth & development*; D014776:Virus Cultivation / Q000379:methods*
D003470:Culture Media