Lodgement of haemopoietic cells in the course of haemopoiesis on cellulose ester membrane: an experimental model for haemopoietic cell trapping.
Intraperitoneally implanted cellulose ester membrane in mice was used as a model to study the lodgement of haemopoietic cells into haemopoietic organs. A heterogeneous cellular coat developed on the membrane. Its upper layer ( epilayer ) thinned out and formed a veil over the cellular coat. Numerous microvilli developed on its free surface. They were randomly oriented. A week after implantation, mice were given total body irradiation (400 cGy) and were infused intraperitoneally with 10(5) marrow cells. Two days after infusion round cells were penetrating the epilayer . Extensive interaction was noted between the surface of the penetrating cells and the microvilli of the epilayer . Penetration was through a single cell in the epilayer and not at the junction of two cells. These observations suggest specific interaction between haemopoietic cells and the supporting tissue leading to lodgement of haemopoietic cells. Intraperitoneally implanted cellulose ester membrane may provide an experimental model for haemopoietic cell trapping.
D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D002482:Cellulose / Q000031:analogs & derivatives; D005260:Female; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D008567:Membranes, Artificial; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D008954:Models, Biological; D010529:Peritoneal Cavity
D008567:Membranes, Artificial; C005062:acetylcellulose; D002482:Cellulose