title: An automatic pulsed laser microfluorometer with high spatial and temporal resolution.
abstract: The paper describes an automatic pulsed laser microfluorometer with high spatial and temporal resolution, developed in our laboratories. The instrument consists of: (i) a nitrogen-laser-pumped dye-laser for the excitation of the fluorescence, (ii) a microscope with additional optics to focus the excitation beam on the sample and to collect the fluorescence, (iii) filters or monochromators to select the output wavelength, (iv) a fast photomultiplier tube to detect the signal, and (v) a dual time-scale microprocessor-controlled signal averager for the acquisition and processing of the signal. Examples are given that show the potential of the time-resolved fluorescence microscopy in studying, quantitatively and qualitatively, the properties of fluorescent molecules.
mesh_terms: D000818:Animals; D001482:Base Composition; D002843:Chromatin / Q000502:physiology* / Q000648:ultrastructure; D004247:DNA / Q000032:analysis; D006801:Humans; D007834:Lasers; D008214:Lymphocytes / Q000166:cytology; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D059828:Plant Cells
chemical_list: D002843:Chromatin; D004247:DNA