Effect of hydrocortisone on immune system of the lizard, Chalcides ocellatus. I. Response of lymphoid tissues and cells to in vivo and in vitro hydrocortisone.
A single dose of 1 mg/g body weight of hydrocortisone acetate (HC) administered intraperitoneally to adult lizards, Chalcides ocellatus induced rapidly a reduction of about 85% of thymic lymphocytes. Histological evidence indicated that cortical, as well as, medullary thymocytes are sensitive to HC exposure. Around 40-50% of lymphocytes in peripheral blood (PB) and spleen were depleted at 3-7 days post-HC injection; such depletion durated about 4 weeks for PB but was rather temporary in spleen. Increase in number of bone marrow (BM) lymphocytes was negligible and transient and could by no way account for the dramatic cell losses in the different lymphoid tissues. The findings thus suggested that HC-mediated lymphocyte depletion in lizards is not attributable to redistribution between the different lymphoid compartments but rather to destruction. In direct conformation, lymphocytes were readily lysed in vitro by 10(-3)M HC, thymocytes being more vulnerable greater than PB greater than spleen greater than BM lymphocytes.
D000818:Animals; D001854:Bone Marrow Cells; D002470:Cell Survival / Q000187:drug effects; D006854:Hydrocortisone / Q000494:pharmacology*; D008116:Lizards / Q000276:immunology*; D008214:Lymphocytes / Q000187:drug effects*; D008221:Lymphoid Tissue / Q000187:drug effects*; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology
D006854:Hydrocortisone