Purification of thirteen toxins active on mice from the venom of the North African scorpion Buthus occitanus tunetanus.
A combination of several equilibrium chromatographic steps permitted us to purify thirteen proteins from the venom of Buthus occitanus tunetanus using the mouse as the test animal for lethality measurement. These proteins have been characterized by their amino acid composition and, for seven of them, by their N-terminal or complete amino acid sequence. The structural data obtained correlate well with the pharmacological and antigenic properties of the neurotoxins.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D012604:Scorpion Venoms / Q000032:analysis*; D014118:Toxins, Biological / Q000302:isolation & purification* / Q000633:toxicity
D000596:Amino Acids; D012604:Scorpion Venoms; D014118:Toxins, Biological