The human preproinsulin gene: synthesis, cloning, gene modification, and expression studies.
A 355 base pair (bp) DNA sequence coding for human preproinsulin has been assembled by joining a synthetic DNA leader sequence coding for 24 preregion amino acids to the previously synthesized DNA duplex of 277 bp constituting the sequence of BCA chain. It was next cloned in M13 mp8 single-stranded bacteriophage and subjected to site-specific mutagenesis and phase shifting to allow its inducible expression under lac operator control. An affinity leader sequence of 25 bp has been added in an attempt to facilitate purification of the preproinsulin.
D001435:Bacteriophages / Q000378:metabolism; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D006801:Humans; D007328:Insulin; D009154:Mutation; D011384:Proinsulin / Q000096:biosynthesis / Q000235:genetics*; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics*
D007328:Insulin; D011498:Protein Precursors; C013737:preproinsulin; D011384:Proinsulin