Lipid-cell interactions. Liposome adsorption and cell-to-liposome lipid transfer are mediated by the same cell-surface sites.
The competitive behavior of solid vs. fluid liposomes in liposome-to-cell adsorption and cell-to-liposome lipid transfer processes was investigated with L cells and FBT epithelial sheets. Binding, transfer and 31P-NMR experiments have demonstrated that: (i) solid liposomes adhere to the cell surface as integral vesicles retaining the entrapped substances; (ii) fluid liposomes are partly disintegrated at the cell surface with concomitant entry of entrapped substances into the cytoplasm, while their lipids remain on the cell surface; (iii) fluid liposomes that escape lysis dissociate from the cell, taking away cell lipid molecules. The latter process underlies the mechanism of cell-to-fluid liposome lipid transfer. In contrast, no lipid transfer occurs between the plasma membrane and solid liposomes. Cell-bound solid liposomes interfere with the transfer of cell lipids to fluid liposomes, while these in turn inhibit the binding of solid liposomes to the cell surface. Moreover, cell-induced aggregation of both fluid and solid freshly added liposomes is also inhibited by preincubation of the cells with either solid or fluid liposomes. Thus, different types of interaction of both fluid and solid liposomes with the cell are mediated by the same (or closely related) sites on the cell surface.
D000327:Adsorption; D000818:Animals; D002462:Cell Membrane / Q000502:physiology*; D004847:Epithelial Cells; D007739:L Cells; D008081:Liposomes; D008560:Membrane Fluidity; D008561:Membrane Fusion; D008563:Membrane Lipids / Q000378:metabolism*; D051379:Mice; D010713:Phosphatidylcholines / Q000502:physiology; D013329:Structure-Activity Relationship
D008081:Liposomes; D008563:Membrane Lipids; D010713:Phosphatidylcholines