Competitive solid-phase immunoassay of testosterone using time-resolved fluorescence.
A competitive solid-phase immunoassay for the determination of testosterone in serum samples using time-resolved fluorescence is described. The solid phase is a testosterone-3-(O-carboxymethyl)-oxime-ovalbumin conjugate coated to polystyrene microtiter strips. Europium-labelled polyclonal and monoclonal antibodies against testosterone-3-(O-carboxymethyl)-oxime-bovine serum albumin were compared. Their behavior was quite similar although the polyclonal antibody was more sensitive, giving a detection limit of 15 fmol testosterone per assay. Correlation with RIA was very good (r = 0.982 and y = -0.150 + 0.969x).
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D005063:Europium; D007118:Immunoassay / Q000379:methods; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D013050:Spectrometry, Fluorescence; D013739:Testosterone / Q000032:analysis*
D000911:Antibodies, Monoclonal; D013739:Testosterone; D005063:Europium