Analysis of heterokaryons and progeny cell hybrids isolated in the absence and in the presence of selective media.
Homokaryons and heterokaryons were formed by polyethylene glycol induced fusion of rat and mouse cells. By following the fusion products, the fate of the individual cells was analyzed. In the absence of selection, a high frequency of the binucleated cells underwent mitosis, giving rise to growing daughter cells. Clones, originating from binucleated cells were isolated and identified as either tetraploid parental cells or hybrid cells. The ratio of hybrids and tetraploid cells was similar to that of heterokaryons and homokaryons among the binucleated cells. Furthermore, the frequency of hybrid colonies was orders of magnitude higher than that obtained in the presence of selective medium (HAT). Hybrids obtained in the absence and presence of selective media showed no consistent differences in chromosome number, HAT resistence, or expression of a non-selected parental isoenzyme, LDH.
D000818:Animals; D002459:Cell Fusion / Q000187:drug effects*; D002460:Cell Line; D002478:Cells, Cultured / Q000502:physiology*; D002999:Clone Cells; D004261:DNA Replication / Q000187:drug effects; D051379:Mice; D009132:Muscles / Q000502:physiology*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats; D014316:Tritium
D014316:Tritium; D011092:Polyethylene Glycols