Separation of radioiodinated angiotensins by chromatofocusing in minicolumns.
Angiotensins I, II, and III (AI, AII, AIII) and Saralasin (Sar1-Ala8-AII) were labeled with 125I and separated from the nonlabeled forms on minicolumns (a Pasteur pipet ) of chromatofocusing medium. At low ionic strength, 125I-labeled angiotensins could be eluted with Polybuffer or a piperazine-histidine buffer at their approximate isoelectric points, while nonlabeled angiotensins remained adsorbed to the column and required 1 mol X liter-1 NaCl for elution. The 125I-labeled angiotensins prepared by this method were bound by antibodies (AI) and adrenal receptors (AII, Saralasin) to an extent similar to angiotensins prepared by DEAE-Sephadex A-25 chromatography. This new method of preparing radioiodinated angiotensins is rapid (15 min), inexpensive, and requires no fraction-collecting equipment.
D000311:Adrenal Glands / Q000378:metabolism; D000803:Angiotensin I / Q000302:isolation & purification; D000804:Angiotensin II / Q000302:isolation & purification; D000805:Angiotensin III / Q000302:isolation & purification; D000809:Angiotensins / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D002852:Chromatography, Ion Exchange; D007457:Iodine Radioisotopes; D007526:Isoelectric Point; D051381:Rats; D011945:Receptors, Angiotensin / Q000378:metabolism; D012504:Saralasin / Q000302:isolation & purification
D000809:Angiotensins; D007457:Iodine Radioisotopes; D011945:Receptors, Angiotensin; D000804:Angiotensin II; D000805:Angiotensin III; D000803:Angiotensin I; D012504:Saralasin