title: Selective elimination of fibroblasts from cultures of normal human melanocytes.
abstract: The main obstacle to establishing pure normal human melanocytes in vitro is contamination of the cultures by fibroblasts. The obstacle can be overcome by selective destruction of fibroblasts with geneticin ( G418 sulfate). Treatment of mixed cultures with this drug at a concentration of 100 micrograms/ml for two days results in pure cultures of normal human melanocytes.
mesh_terms: D002455:Cell Division; D002469:Cell Separation / Q000379:methods; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D005839:Gentamicins / Q000633:toxicity; D006801:Humans; D007700:Kinetics; D008544:Melanocytes / Q000166:cytology* / Q000502:physiology
chemical_list: D003470:Culture Media; D005839:Gentamicins; C010680:antibiotic G 418