title: Age-related alteration in catecholamine activity within microdissected brain regions of ovariectomized Fischer 344 rats.
abstract: The effects of increasing age on catecholamine (CA) metabolism in microdissected brain regions and on serum and pituitary hormone levels were examined in ovariectomized Fischer 344 rats. Young (4 to 5 months old) and middle-aged (9 to 10 months old) normally cycling and old repeated pseudopregnant rats (21-22 months old, PP) were ovariectomized to eliminate the complicating effects of cyclic gonadal steroid fluctuations. CA metabolism was examined 2 weeks later. To determine CA turnover rates, each age-group was subdivided into three groups, which were killed by decapitation 0, 45, or 90 min after administration of alpha-methyl-para-tyrosine (alpha-mpt). Dopamine (DA) and norepinephrine (NE) concentrations were determined in microdissected brain regions by radioenzymatic assay, and turnover rates were estimated. Steady-state concentrations of NE were not altered in middle-aged rats, but NE turnover rates increased in middle-aged rats in five of the six areas examined. While NE concentrations did not change with age in the median eminence (ME), NE turnover rates increased significantly in the two older age groups. These data indicate that the age-related decline in NE concentrations in several ventral diencephalic nuclei is preceded by a period of hyperactivity in noradrenergic neurons. DA concentrations were generally decreased in most areas examined in old versus young rats, with dramatic DA depletions (42-78%) observed in five regions. However, no consistent relationship between DA concentrations and turnover rates was seen either in regions with stable DA levels or in those which showed an age-associated decrease in DA concentrations. In the ME, a 42% decline in DA concentration was associated with an increase in the DA turnover rate in the oldest group of rats. Serum luteinizing hormone (LH) levels were similar in all three age groups of ovariectomized rats, while serum prolactin was elevated four-fold in old compared to younger animals. These data indicate that a complex pattern of regional alterations in CA metabolism accompanies the aging process and these may be related to the pseudopregnant state and hormone secretory capacity of aging Fischer 344 rats.
mesh_terms: D000367:Age Factors; D000818:Animals; D001923:Brain Chemistry; D002369:Castration; D004298:Dopamine / Q000032:analysis*; D005260:Female; D007986:Luteinizing Hormone / Q000097:blood; D009638:Norepinephrine / Q000032:analysis*; D010053:Ovary / Q000502:physiology*; D011388:Prolactin / Q000097:blood; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D011388:Prolactin; D007986:Luteinizing Hormone; D004298:Dopamine; D009638:Norepinephrine