A rapid solid phase assay for the detection of circulating immune complexes.
A simplified process, which we have termed Enzyme Immune Complex Assay (EICATM) for the detection of circulating immune complexes (CICs), is described herein. The method utilizes readily available reagents, small quantities of serum, and can be performed quickly with a minimal amount of equipment. Serum from 38 normal controls, 98 burn patients, 36 frostbite injury patients, and 21 patients with elevated rheumatoid factor (RF) were tested for CICs in an immune function study. Elevated immune complex levels were found in the group of patients with frostbite injury, and in the group with elevated RF. Serum from thermally injured patients had slightly depressed yet normal CIC levels. The detection of elevated CICs by the EICATM method compared favorably with the more cumbersome Raji cell method, with the added advantage of simplicity, speed, and the ability to detect non-IgG immune complexes.
D000936:Antigen-Antibody Complex / Q000032:analysis*; D001327:Autoimmune Diseases / Q000276:immunology; D002056:Burns / Q000276:immunology; D005627:Frostbite / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D011092:Polyethylene Glycols
D000936:Antigen-Antibody Complex; D011092:Polyethylene Glycols