Determination of urochloralic acid, the glucuronic acid conjugate of trichloroethanol, by gas chromatography with electron-capture detection and its application to urine, plasma and liver.
Gas chromatography with electron-capture detection was used to quantify the glucuronic acid conjugate of trichloroethanol, urochloralic acid. The conjugate was extracted into ethyl acetate--ethanol (19:1, v/v) under acidic conditions, and analyzed as its trifluoroacetylated methyl ester or as its acetylated methyl ester. As little as 0.2 microgram of the conjugate was quantified, and the method was applicable to urine, plasma and liver. The synthesis of urochloralic acid from trichloroethanol by rat liver microsomal fraction was demonstrated in the presence of uridine diphosphoglucuronic acid as coenzyme.
D000089:Acetic Anhydrides; D000107:Acetylation; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D003067:Coenzymes / Q000378:metabolism; D005463:Fluoroacetates; D005965:Glucuronates / Q000032:analysis* / Q000097:blood / Q000652:urine; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008099:Liver / Q000032:analysis* / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014269:Trifluoroacetic Acid / Q000494:pharmacology; D014535:Uridine Diphosphate Glucuronic Acid / Q000378:metabolism
D000089:Acetic Anhydrides; D003067:Coenzymes; D005463:Fluoroacetates; D005965:Glucuronates; D014535:Uridine Diphosphate Glucuronic Acid; C017958:trifluoroacetic anhydride; D000255:Adenosine Triphosphate; D014269:Trifluoroacetic Acid; C041653:urochloralic acid