Species differences in the occurrence of copper-metallothionein in the particulate fractions of the liver of copper-loaded animals.
Large amounts of Cu-metallothionein were obtained by 2-mercaptoethanol and sodium dodecyl sulphate extractions of the particulate fractions of the liver of pigs given high-Cu2+ diets or rats injected with Cu2+. Three isoproteins were purified from pig liver and characterized on the basis of their physicochemical properties, metal content and amino acid composition. No such pool of Cu-metallothionein was present in the liver of Cu2+-loaded sheep or of rats given Cu2+-supplemented diets.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002458:Cell Fractionation; D002850:Chromatography, Gel; D003300:Copper / Q000378:metabolism* / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008668:Metallothionein / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012756:Sheep; D013045:Species Specificity; D013552:Swine
D000596:Amino Acids; D003300:Copper; D008668:Metallothionein