Influence of the culture time on DNA damage and repair in isolated rat hepatocytes exposed to nitrochlorobenzene derivatives.
The induction of DNA damage on 1.5- and 24-h cultured hepatocytes was tested after a 3-h exposure to 5 and 50 microM mono-, di-, and trinitrochlorobenzene (100-00-5; 97-00-7; 88-88-0). DNA-repair synthesis, elicited by nitrochlorobenzene treatment, was also estimated 24 and 48 h after the withdrawal of the nitro-aryl halides. DNA damage and repair were evaluated by determining the DNA elution rate in alkali. A dose-related rate of DNA damage was obtained by exposure of 1.5-h-cultured hepatocytes to 5 and 50 microM nitrochlorobenzenes . DNA of 24-h-cultured cells was not affected by nitrochlorobenzene treatment. The data obtained by exposure to 5 microM methyl methanesulfonate (66-27-3) and nitrosodimethylamine (62-75-9), direct and indirect methylating agents, suggest that 24-h-cultured liver cells are still able to transform nitrosodimethylamine but not nitrochlorobenzenes . Isolated hepatocytes maintain their capability of repairing the induced DNA damage when cultured for 24 and 48 h in fresh medium. The system offers an interesting model to investigate the perturbations related to the metabolism of xenobiotics.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics; D004260:DNA Repair; D004128:Dimethylnitrosamine / Q000494:pharmacology; D004137:Dinitrochlorobenzene / Q000494:pharmacology*; D008099:Liver / Q000166:cytology; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D009154:Mutation / Q000187:drug effects*; D009578:Nitrobenzenes / Q000494:pharmacology*; D010853:Picryl Chloride / Q000494:pharmacology*; D051381:Rats; D013997:Time Factors
D004137:Dinitrochlorobenzene; D009578:Nitrobenzenes; D004247:DNA; D008741:Methyl Methanesulfonate; C010407:4-chloronitrobenzene; D004128:Dimethylnitrosamine; D010853:Picryl Chloride