title: Inactivation of rabies virus in reagents used for the fluorescent rabies antibody test.
abstract: Procedures for inactivating rabies virus in reagents used for the fluorescent rabies antibody test are described. Mouse brain adsorbing suspensions containing greater than or equal to 10(9) 50% lethal doses of virus per ml were rendered noninfectious by treatment with 0.1% beta-propiolactone or by heating at 56 degrees for greater than or equal to 30 min. Viable virus in tissue impression smears was inactivated by acetone fixation at 50 degrees C for greater than or equal to 30 min or by immersion in 0.1% beta-propiolactone at 37 degrees C for 2 h. Inactivated reagents gave specific and sensitive reactions in the fluorescent rabies antibody test.
mesh_terms: D000096:Acetone; D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis; D005404:Fixatives; D005455:Fluorescent Antibody Technique; D006358:Hot Temperature; D007202:Indicators and Reagents; D051379:Mice; D011420:Propiolactone; D011818:Rabies / Q000175:diagnosis*; D011820:Rabies virus / Q000254:growth & development* / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D005404:Fixatives; D007202:Indicators and Reagents; D000096:Acetone; D011420:Propiolactone