A rapid method for determination of acetylation phenotype using dapsone.
A rapid, simple one-stage protein precipitation method for the estimation of plasma dapsone (DDS) and monoacetyldapsone (MADDS) concentration by high-pressure liquid chromatography (h.p.l.c.) is described. Its performance in the assignment of acetylator phenotype was compared with a reference h.p.l.c. method utilising an extraction procedure and internal standard. The rapid h.p.l.c. technique combined with measurement of the plasma MADDS/DDS ratio is, in our opinion, the method of choice for the determination of the acetylator phenotype in population studies.
D000107:Acetylation; D002625:Chemistry, Organic; D002851:Chromatography, High Pressure Liquid; D003622:Dapsone / Q000031:analogs & derivatives / Q000097:blood; D006801:Humans; D055601:Organic Chemistry Phenomena; D010641:Phenotype
D003622:Dapsone; C018965:monoacetyldapsone