Capsaicin pretreatment inhibits vagal cholinergic and non-cholinergic control of pulmonary mechanics in the guinea pig.
The effects of vagal nerve stimulation, ether, capsaicin, histamine and substance P (SP) on lung resistance (RL) and dynamic lung compliance (CDYN) were studied in anaesthetized guinea pigs. The in vivo responses in control animals were compared with the effects after systemic pretreatment with capsaicin or local application of capsaicin on the cervical vagal nerves. Vagal nerve stimulation induced an increase in RL and a fall in CDYN. Significant changes in RL and CDYN (more than 50%) were still present after atropine, while the vagal heart response was abolished. Systemic or local capsaicin pretreatment abolished the atropine-resistant vagal effect on RL and CDYN. The atropine-sensitive changes in pulmonary mechanics upon vagal stimulation were significantly reduced by capsaicin pretreatment. The increase in RL and a fall in CDYN induced by ether and capsaicin was significantly reduced or abolished by systemic capsaicin pretreatment, while the histamine-induced bronchoconstriction was only slightly reduced. Ether and capsaicin seemed also to activate capsaicin-sensitive neurons of non-vagal origin since they still caused large effects in animals, which had been locally pretreated with capsaicin on the vagal nerves. The increased RL and decreased CDYN induced by SP was unchanged in capsaicin-pretreated animals. The tracheal tension increase in vitro induced by acetylcholine and histamine was uninfluenced by systemic capsaicin pretreatment. The atropine-sensitive tracheal contraction and atropine-resistant relaxation upon field stimulation seemed also to be unchanged after capsaicin pretreatment.(ABSTRACT TRUNCATED AT 250 WORDS)
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001285:Atropine / Q000494:pharmacology*; D001980:Bronchi / Q000502:physiology; D002211:Capsaicin / Q000494:pharmacology*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D004558:Electric Stimulation; D005260:Female; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D008170:Lung Compliance / Q000187:drug effects*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000502:physiology*; D011976:Receptors, Muscarinic / Q000502:physiology; D012129:Respiratory Function Tests; D014132:Trachea / Q000502:physiology; D014630:Vagus Nerve / Q000187:drug effects / Q000502:physiology*
D011976:Receptors, Muscarinic; D001285:Atropine; D006632:Histamine; D002795:Choline O-Acetyltransferase; D000109:Acetylcholine; D002211:Capsaicin