title: Evidence for existence of somatostatin-like immunoreactivity with molecular heterogeneity in porcine ovaries.
abstract: Measurement of somatostatin (SRIF) by RIA in porcine ovarian tissues after removal of the follicular fluid revealed the existence of immunoreactive (IR) SRIF at concentrations of 2.2 +/- 0.6 ng/g wet weight (mean +/- SD). On gel chromatographic analysis of ovarian extracts one major and two minor components with SRIF-like immunoreactivities have been detected. The major component comprised 59.5 +/- 15.1% of the total IR-SRIF and was coeluted with synthetic tetradecapeptide (SRIF 14). The two other components seemed to have almost the same molecular weights as synthetic octacosapeptide (SRIF 28) (25.1 +/- 10%) and pro-somatostatin (15.4 +/- 5.5%), respectively. It is concluded that SRIF in porcine ovaries is heterogeneous, comprising SRIF 14 as a major component, as well as larger forms of SRIF which probably represent SRIF precursors.
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D005260:Female; D008970:Molecular Weight; D010053:Ovary / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011863:Radioimmunoassay; D013552:Swine
chemical_list: D010455:Peptides; C029993:somatostatin-like peptides