Induction of peripheral blood eosinophilia in mice by excretory and secretory antigen of Toxocara canis larvae.
Medium in which T. canis larvae had been cultured was applied to a Sephadex G-25 gel column and four fractions (Fr. 1-Fr. 4) were isolated. Each fraction was administered to mice by implanting a mini-osmotic pump into the peritoneal cavity and the subsequent peripheral blood eosinophilic counts were recorded. Mice given Fr. 1 showed a markedly greater eosinophilic response than did those given the other fractions. Complement activation by each fraction was investigated using normal human serum. Fr. 1 and Fr. 3 had a capacity to activate complement. The results suggest that Fr. 1 may be not only antigenetic and allergenic but also could induce eosinophilia and activate complement.
D000818:Animals; D000941:Antigens / Q000276:immunology*; D001196:Ascariasis / Q000276:immunology*; D003167:Complement Activation; D004802:Eosinophilia / Q000276:immunology*; D004804:Eosinophils / Q000276:immunology; D006801:Humans; D007814:Larva / Q000276:immunology; D008297:Male; D051379:Mice; D014119:Toxocara / Q000276:immunology*; D014120:Toxocariasis / Q000276:immunology*
D000941:Antigens