Activation of the rat liver androgen-receptor complex.
Activation of androgen receptor in rat liver cytosol was studied in vitro. The state of activation was judged by binding of [3H] R1881-receptor complex to chromatin. High ionic strength (0.4 M KCl as a final concentration) provoked the binding of [3H] R1881-receptor complex to chromatin at 0 degrees C. At low ionic strength, activation was very slow at 0 degrees C, but was very rapid at 25 degrees C and reached the maximum at 15 min of heating.
D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D006358:Hot Temperature; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D009994:Osmolar Concentration; D011189:Potassium Chloride / Q000494:pharmacology; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D011944:Receptors, Androgen / Q000187:drug effects / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*
D002843:Chromatin; D011944:Receptors, Androgen; D011987:Receptors, Steroid; D011189:Potassium Chloride