The glycoconjugates from Trypanosoma Cruzi, purification and composition of a glycoprotein.
1. The complex of glycoconjugates extracted with phenol-water from culture epimastigotes of a non-infective line (To) of Trypanosoma cruzi, derived from the Tulahuén strain showed only two components by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 2. The slower migrating glycoconjugate, a glycoprotein of molecular weight 45, 000 was purified to apparent homogeneity. 3. A content of 76% protein, 17% neutral sugars and 6% glucosamine was analysed. Galactose and mannose occur in a ratio of 3:1 as determined by gas-liquid chromatography. A trace of glucose was detected in some samples.
D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification*; D046911:Macromolecular Substances; D008970:Molecular Weight; D014349:Trypanosoma cruzi / Q000032:analysis*
D002241:Carbohydrates; D006023:Glycoproteins; D046911:Macromolecular Substances