title: Prevention by 3-N-ribosyluric acid of the oxidation of bovine hemoglobin by sodium nitrite.
abstract: The addition of sodium nitrite to washed bovine erythrocytes incubated in buffered saline resulted in the formation of methemoglobin with a decrease in the concentration of 3-N-ribosyluric acid. The oxyhemoglobin in hemolysates prepared from bovine red cells which contained high concentrations of 3-ribosyluric acid was oxidized to methemoglobin more slowly than oxyhemoglobin from cells with low levels of 3-ribosyluric acid. Oxyhemoglobin from hemolysates that were dialyzed was oxidized more rapidly than oxyhemoglobin which was not dialyzed. 3-Ribosyluric acid, glutathione, uric acid, and ascorbic acid prevented the oxidation of oxyhemoglobin by nitrite. Uric acid protected oxyhemoglobin at the lowest concentration, followed closely by ascorbic acid, 3-ribosyluric acid, and glutathione. Hydrogen peroxide enhanced the oxidation produced by nitrite; this effect was also prevented by the four antioxidants used.
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002417:Cattle; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005978:Glutathione / Q000494:pharmacology; D006461:Hemolysis; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D008706:Methemoglobin / Q000096:biosynthesis; D009573:Nitrites / Q000037:antagonists & inhibitors*; D010084:Oxidation-Reduction / Q000187:drug effects; D010108:Oxyhemoglobins / Q000378:metabolism*; D012263:Ribonucleosides / Q000494:pharmacology*; D012977:Sodium Nitrite / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D014527:Uric Acid / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D009573:Nitrites; D010108:Oxyhemoglobins; D012263:Ribonucleosides; C012090:uric acid riboside; D014527:Uric Acid; D008706:Methemoglobin; D006861:Hydrogen Peroxide; D005978:Glutathione; D012977:Sodium Nitrite; D001205:Ascorbic Acid