Salmon and human calcitonin-like peptides in man.
Calcitonin-like peptides have been identified in the serum of normal subjects and of medullary thyroid carcinoma (MTC) patients. Using specific homologous radioimmunoassays (RIA) in combination with reversed-phase high performance liquid chromatography and gel permeation chromatography under denaturing conditions, we have recognized major components which coeluted with human calcitonin-(1-32), PDN-21, a carboxyl-terminal flanking peptide derived from the calcitonin mRNA sequence, and salmon calcitonin-(1-32). An additional 12000 molecular weight peak possibly represents a human calcitonin-PDN-21 polyprotein. In both the human calcitonin-(1-32) (normal value less than 0.043 ngEq/ml; MTC 140 +/- 80 ngEq/ml, mean value +/- SEM) and the PDN-21 (normal value less than 0.050 ngEq/ml; MTC 33.6 +/- 16.5 ngEq/ml) RIAs, serum levels were increased in MTC patients. Circulating levels of the salmon calcitonin-like peptide were indistinguishable between normal subjects (0.038 +/- 0.006 ngEq/ml) and MTC patients (0.037 +/- 0.011 ngEq/ml).
D002116:Calcitonin / Q000097:blood*; D002277:Carcinoma / Q000097:blood; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D010455:Peptides / Q000097:blood*; D011089:Polydeoxyribonucleotides / Q000097:blood; D011863:Radioimmunoassay; D013964:Thyroid Neoplasms / Q000097:blood
D010455:Peptides; D011089:Polydeoxyribonucleotides; C028815:salmon calcitonin; D002116:Calcitonin