Interactions of caffeine and phenobarbital on sister chromatid exchanges in rat liver epithelial cell lines treated with 2-acetyl-aminofluorene and N-acetoxy-2-acetylaminofluorene.
Using "Fluorescence plus Giemsa" technique, sister chromatid exchanges (SCE) were determined on second-division metaphases in rat liver epithelial cell lines treated with 2-acetylaminofluorene (2-AAF), the procarcinogen, and N-acetoxy-2-AAF (N-OAc-2AAF), an ultimate carcinogen analog. The SCE frequency was found decreased after some conditions of 2-AAF treatment and increased with N-OAc-2-AAF. Phenobarbital (PB) decreased also the SCE frequency but cancelled the 2-AAF action when incubated after the procarcinogen. The addition of caffeine (Caf) cancelled the action of 2-AAF but not of phenobarbital. It is suggested that the mechanism of SCE may have several origins.
D015073:2-Acetylaminofluorene / Q000031:analogs & derivatives* / Q000633:toxicity*; D000099:Acetoxyacetylaminofluorene / Q000633:toxicity*; D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D003434:Crossing Over, Genetic / Q000187:drug effects*; D004847:Epithelial Cells; D004848:Epithelium / Q000648:ultrastructure; D008099:Liver / Q000187:drug effects* / Q000648:ultrastructure; D008677:Metaphase / Q000187:drug effects; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D002110:Caffeine; D000099:Acetoxyacetylaminofluorene; D015073:2-Acetylaminofluorene; D010634:Phenobarbital