Rotational dynamics of immunoglobulins with fluorescent haptens on a membrane surface.
The rotational dynamics of rabbit immunoglobulin G with fluorescent lipid haptens on a membrane surface has been studied by nanosecond fluorescence emission anisotropic spectroscopy. It has been found that the rotational angles of the antibody are very restricted on the membrane, but that the rotation rate itself is not appreciably lower than that in solution, and is independent of the membrane fluidity.
D000818:Animals; D005456:Fluorescent Dyes; D006241:Haptens; D007074:Immunoglobulin G; D007700:Kinetics; D008560:Membrane Fluidity; D009282:Naphthalenesulfonates; D011817:Rabbits; D012399:Rotation; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D006241:Haptens; D007074:Immunoglobulin G; D009282:Naphthalenesulfonates; C006421:1,5-I-AEDANS