Phosphorylation of histones in cells treated with hypertonic and acidic media.
Factors in the extracellular environment, specifically hypertonic or acidic growth media, are shown to alter the modification of histones in several cell lines. For histone 2A, changes in modification were visible in the mass pattern and were found to be primarily changes in phosphorylation. The increased modification of the core histones was quickly reversed when cells were returned to normal medium.
D000107:Acetylation; D000818:Animals; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007939:Leukemia L1210 / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D009994:Osmolar Concentration; D010766:Phosphorylation
D003470:Culture Media; D006657:Histones