Complex structure of human bronchial mucus glycoprotein.
Human bronchial mucus glycoproteins or mucins were isolated from the sputum of two patients by a method avoiding reducing agents and involving water extraction and gel filtration on Sepharose CL-2B in 6 M guanidinium chloride. The chemical analysis indicated approximately 25-40% lipid. The amino acid and carbohydrate analysis differ quantitatively from that of mucins purified after prior reduction of mucus. These fractions also have a higher proportion of aspartic and glutamic acids than that of the mucins from reduced sputum. These mucins are still contaminated by small amounts of peptides but do not seem to contain disulfide-attached cross-linking protein. Human bronchial mucins have a strong tendency to form aggregates except in 6 M guanidinium chloride. Electron microscopy performed with various procedures indicates the presence of both micelles and flexible threads measuring 200-1000 nm. Delipidation removes most of the micellar forms. Thereafter mucins appear mainly as polydisperse flexible extended threads and also as aggregates. These features of bronchial mucins do not fit with the generally accepted idea of mucin subunits linked by disulfide bridges (unless they are linked end to end) and alternatively favour a model where mucin molecules behave like filaments that could easily aggregate according to the solvent system (mucin concentration, absence of dissociating conditions).
D000596:Amino Acids / Q000032:analysis; D001980:Bronchi / Q000032:analysis*; D001991:Bronchitis / Q000378:metabolism*; D002241:Carbohydrates / Q000032:analysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002648:Child; D002908:Chronic Disease; D003550:Cystic Fibrosis / Q000378:metabolism*; D006023:Glycoproteins / Q000032:analysis*; D019791:Guanidine; D006146:Guanidines; D006801:Humans; D008055:Lipids / Q000032:analysis; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D009077:Mucins / Q000032:analysis*; D009093:Mucus / Q000032:analysis*; D010084:Oxidation-Reduction
D000596:Amino Acids; D002241:Carbohydrates; D006023:Glycoproteins; D006146:Guanidines; D008055:Lipids; D046911:Macromolecular Substances; D009077:Mucins; D019791:Guanidine