Bioconversion of alpha-[14C]zearalenol and beta-[14C]zearalenol into [14C]zearalenone by Fusarium roseum 'Gibbosum'.
Cultures of Fusarium roseium 'Gibbosum' on rice were treated with [14C]zearalenone, alpha[14C]zearalenol, or beta-[14C]zearalenol to determine whether a precursor-product relationship exists among these closely related fungal metabolites. Culture extracts were purified by silica gel column chromatography and fractionated by high-pressure liquid chromatography, and the level of radioactivity was determined. Within 7 days, the beta-[14C]zearalenol was converted to zearalenone, and no residual beta-[14C]zearalenol was detectable. Most of the alpha-[14C]zearalenol added was also converted into zearalenone with 14 days. In cultures treated with [14C]zearalenone, no radioactivity was noted in any other components.
D002250:Carbon Radioisotopes / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D005670:Fusarium / Q000378:metabolism*; D012118:Resorcinols / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D015029:Zeranol / Q000031:analogs & derivatives / Q000378:metabolism*
D002250:Carbon Radioisotopes; D003470:Culture Media; D012118:Resorcinols; C029659:zearalenol; D015029:Zeranol