The structure of the carbohydrate units of the carboxyl-terminal peptide of procollagen as elucidated by 500 MHz 1H-NMR spectroscopy.
The oligosaccharides of chick embryo type I procollagen were isolated from carboxyl-terminal propeptide fragment by exhaustive digestion with papain and pronase, and then purified as a mixture of glycopeptides. The structures of the oligosaccharides were established by high-resolution 1H-NMR spectroscopy and found to be a mixture with respect to the non-reducing terminal residues as shown below: (formula; see text) The percentages refer to the relative amount of those mannose residues present in the mixture. The data suggest that the oligosaccharides are a microheterogeneous mixture of high-mannose type glycans containing between six and nine mannose residues per carbohydrate unit. Such carbohydrate chains, although not uncommon for glycoproteins, had never been found before for collagen or collagen-related compounds.
D000595:Amino Acid Sequence; D000818:Animals; D002240:Carbohydrate Sequence; D002642:Chick Embryo; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000032:analysis; D010206:Papain / Q000378:metabolism; D011347:Procollagen / Q000032:analysis*; D011402:Pronase / Q000378:metabolism
D009844:Oligosaccharides; D011347:Procollagen; D010206:Papain; D011402:Pronase