[Isolation and structural studies of glucans from Phytophthora parasitica].
Several polysaccharides have been isolated from the cell walls of Phytophthora parasitica, a phytopathogenic fungus of carnation. The crude polysaccharides were fractionated by successive chromatographies on DEAE-cellulose, Sephadex G-25, concanavalin-A-Sepharose and Sephadex G-200 columns. The neutral polysaccharides consist of a mixture of beta(1----3, 1----6)-D-glucans whose relative molecular masses varied from 9000 to about 200 000. All these polysaccharides have a main chain of beta(1----3)-linked D-glucose residues. They differ by the presence of 1----6 branched chains consisting of D-glucose and D-Glc-(1----3)-D-Glc, for the lowest molecular mass polysaccharides or D-Glc-(1----3)-D-Glc-(1---3)-D-Glc for the highest molecular mass polysaccharides.
D002473:Cell Wall / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D005658:Fungi / Q000032:analysis*; D005936:Glucans / Q000032:analysis*; D008970:Molecular Weight; D010838:Phytophthora / Q000032:analysis* / Q000648:ultrastructure; D011134:Polysaccharides / Q000032:analysis*
D005936:Glucans; D011134:Polysaccharides
Isolement et étude structurale de glucanes de Phytophthora parasitica.