High-mannose oligosaccharides from human Tamm-Horsfall glycoprotein.
The present communication reports the occurrence of high-mannose oligosaccharides on Tamm-Horsfall glycoprotein prepared from human pooled urine. The Pronase digest of the glycoprotein was fractionated by gel filtration and a high-mannose glycopeptide species was separated from complex-type glycopeptides. When high-mannose glycopeptides were digested with endo-beta-N-acetylglucosaminidase H, followed by reduction with [3H]KBH4, three oligosaccharides were resolved by thin-layer chromatography. On the basis of chromatographic mobility and exoglycosidase digestions the composition Man7-, Man6-, and Man5-GlcNAc was assigned to the three oligosaccharides. Man6GlcNAc is by far the major component.
D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D006801:Humans; D008358:Mannose / Q000032:analysis*; D009088:Mucoproteins / Q000302:isolation & purification / Q000652:urine*; D009844:Oligosaccharides / Q000302:isolation & purification*; D058949:Uromodulin
D009088:Mucoproteins; D009844:Oligosaccharides; C548612:UMOD protein, human; D058949:Uromodulin; D008358:Mannose