Determination of malonyl-coenzyme A in rat heart, kidney, and liver: a comparison between acetyl-coenzyme A and butyryl-coenzyme A as fatty acid synthase primers in the assay procedure.
The malonyl-CoA assay was nonlinear at low malonyl-CoA concentrations when labeled acetyl-CoA was used as fatty acid synthase primer. Linearity was obtained with low concentrations of both fatty acid synthase and labeled acetyl-CoA, but then the assay was disturbed by the diluting effect of endogenous acetyl-CoA. The problems of nonlinearity and dilution of radioactivity by endogenous compounds were absent when labeled butyryl-CoA was used as primer. The levels of malonyl-CoA in rat heart, kidney, and liver were determined. The use of butyryl-CoA gave higher values of malonyl-CoA.
D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000214:Acyl Coenzyme A / Q000032:analysis* / Q000378:metabolism*; D000818:Animals; D064429:Fatty Acid Synthases / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D008316:Malonyl Coenzyme A / Q000032:analysis*; D009206:Myocardium / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
D000214:Acyl Coenzyme A; C024343:butyryl-coenzyme A; D008316:Malonyl Coenzyme A; D000105:Acetyl Coenzyme A; D064429:Fatty Acid Synthases