Styrene metabolism in man: gas chromatographic separation of mandelic acid enantiomers in the urine of exposed persons.
D- and L-mandelic acid are separated by gas chromatography as isopropyl ester or isopropyl ester-isopropyl urethane on capillary columns, coated with Chirasil -Val. For its determination in urine the isopropyl ester procedure gives better results because other components of the urine do not cause interference. Thus D- and L-mandelic acid in the urine of exposed workers could be detected and verified by means of GC/MS. Occupational styrene exposure near the MAK-value (100 ppm) results in a L/D-enantiomer-ratio in urine of nearly 1.5.
D002849:Chromatography, Gas / Q000379:methods; D005260:Female; D006038:Glyoxylates / Q000652:urine; D006626:Hippurates / Q000652:urine; D006801:Humans; D008297:Male; D008333:Mandelic Acids / Q000652:urine*; D009784:Occupational Diseases / Q000652:urine*; D013237:Stereoisomerism; D020058:Styrene; D013343:Styrenes / Q000378:metabolism*
D006038:Glyoxylates; D006626:Hippurates; D008333:Mandelic Acids; D013343:Styrenes; C012482:phenylglyoxylic acid; D020058:Styrene; C030514:hippuric acid