title: Effect of pH, temperature and media on acid and alkaline phosphatase activity in "clinical" and "nonclinical" isolates of Bordetella bronchiseptica.
abstract: Twenty-two isolates of Bordetella bronchiseptica were studied to determine the effects of pH, incubation temperature and type of media on peak acid and alkaline phosphatase activity. The pH optimum for alkaline phosphatase activity was 9.0 for all of the isolates tested. The pH optimum for acid phosphatase activity was 5.8 for 67% of the isolates and 4.8 for 33%. All of the isolates showed peak phosphatase activity at 37 degrees C. No preference was shown in 35% of the isolates between the types of media tested; however, 40% preferred tryptose broth, 20% preferred nutrient broth and 5% preferred brain-heart infusion broth. No relationship was shown between phosphatase activity and the mouse lethality of the isolates.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001884:Bordetella / Q000201:enzymology* / Q000472:pathogenicity; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D051379:Mice; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000662:veterinary*; D013552:Swine / Q000382:microbiology*; D013553:Swine Diseases / Q000382:microbiology*; D013696:Temperature
chemical_list: D003470:Culture Media; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase