DNA binding spectrum of the carcinogen N-acetoxy-N-2-acetylaminofluorene significantly differs from the mutation spectrum.
The 3'----5' exonuclease activity of bacteriophage T4 DNA polymerase is found to be blocked in the vicinity of the N-2-acetylaminofluorene (-AAF) adducts to DNA. This observation allowed us to determine the binding spectrum of the -AAF adducts along a given DNA sequence. The mutation spectrum in a forward mutation assay within this same sequence has been established. Comparison between the -AAF binding spectrum and the mutation spectrum shows that there is no direct correlation.
D015073:2-Acetylaminofluorene / Q000031:analogs & derivatives* / Q000494:pharmacology; D000099:Acetoxyacetylaminofluorene / Q000378:metabolism / Q000494:pharmacology*; D001345:Autoradiography; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D009154:Mutation; D010957:Plasmids / Q000187:drug effects*; D013604:T-Phages / Q000201:enzymology
D000099:Acetoxyacetylaminofluorene; D004247:DNA; D015073:2-Acetylaminofluorene; D004259:DNA-Directed DNA Polymerase