Thymidylate synthetase-positive and -negative murine mammary FM3A carcinoma cells as a useful system for detecting thymidylate synthetase inhibitors.
The murine mammary FM3A/O and the thymidylate (dTMP) synthetase-deficient FM3A/TS- carcinoma cell lines can be considered as a novel and useful test system for the detection of nucleoside analogues which are directly aimed at the thymidylate synthetase. These compounds should be inhibitory for FM3A/O but not for FM3A/TS- cells, and their inhibitory effects on FM3A/O cell growth should be readily reversed by exogenous dThd within the concentration range of 5-20 microM.
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D002478:Cells, Cultured; D003857:Deoxyuridine / Q000031:analogs & derivatives / Q000494:pharmacology; D007939:Leukemia L1210 / Q000201:enzymology; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology*; D008780:Methyltransferases / Q000037:antagonists & inhibitors*; D051379:Mice; D013940:Thymidylate Synthase / Q000037:antagonists & inhibitors* / Q000172:deficiency
D000964:Antimetabolites, Antineoplastic; D008780:Methyltransferases; D013940:Thymidylate Synthase; D003857:Deoxyuridine