Analysis of the outer membrane proteins of Bacteroides nodosus, the causal organism of ovine footrot.
Examination by SDS-PAGE of lithium acetate extracts of several strains of depiliated Bacteroides nodosus revealed 6 major outer membrane proteins (including pilin). The 5 membrane proteins exhibited approximate molecular weights of 75000, 50000, 38000, 34500 and 26500 whereas pilin had a MW of 17500 for the majority of strains. All proteins were accessible to lactoperoxidase-catalysed iodination and proteins 1, 2 and 5 were shown to be glycoproteins. Several attempts to isolate individual OMC proteins in pure form by selective solubilization and gel filtration were unsuccessful, but electroelution of individual outer membrane complex proteins resolved by SDS-PAGE provided sufficient quantities of antigen for immunization of sheep and for immunochemical analysis.
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001345:Autoradiography; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D001439:Bacteroides / Q000032:analysis* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005535:Foot Rot / Q000382:microbiology*; D007106:Immune Sera; D007114:Immunization / Q000662:veterinary; D008565:Membrane Proteins / Q000032:analysis*; D008970:Molecular Weight; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D007106:Immune Sera; D008565:Membrane Proteins