[Age-related decrease of the binding of isolated rat liver and brain nuclei with glucocorticoid-receptor complexes].
Isolated nuclei from liver and brain of rats of various age bind isolated and 1000-2000-fold purified glucocorticoid-receptor complexes (GRC) containing [3H]dexamethasone. The binding of GRC by the nuclei decreases with an increase in the NaCl concentration from 0 to 0.4 M in a medium. The maximal degree of GRC binding is observed in the liver and brain nuclei of newborn rats (1-day-old); the nuclei of adult animals aged 3 months bind GRC 1/2 times as much. in old (25-month-old) and grown-up animals, no significant differences in GRC binding by nuclei from respective tissues were revealed. Brain nuclei of newborn rats were shown to bind GRC to a greater extent than liver nuclei.
D000375:Aging; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid
Vozrastnoe umen'shenie sviazyvaniia izolirovannymi iadrami pecheni i mozga krysy gliukokortikoid-retseptornykh kompleksov.