Separation and quantitation of perbenzoylated glucocerebroside and galactocerebroside by high-performance liquid chromatography.
Perbenzoylated gluco- and galactocerebrosides were separated and quantitated with a modification of existing HPLC methodology. A linear gradient of 0.9-18.4% dioxane in hexane was used to separate the derivatives on a pellicular silica column heated to 65 degrees C. The elevated temperature was the major modification that permitted the separation. This system was compared to a previously reported silica column/isopropanol in hexane system.
D000818:Animals; D001565:Benzoates; D001923:Brain Chemistry; D002554:Cerebrosides / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005699:Galactosylceramides / Q000302:isolation & purification*; D005776:Gaucher Disease / Q000378:metabolism; D005963:Glucosylceramides / Q000302:isolation & purification*; D006801:Humans; D007668:Kidney / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D011919:Rats, Inbred Strains; D013696:Temperature
D001565:Benzoates; D002554:Cerebrosides; D005699:Galactosylceramides; D005963:Glucosylceramides; C013409:benzoyl chloride