title: Monoamine oxidase, an intracellular probe of oxygen pressure in isolated cardiac myocytes.
abstract: The activity of monamine oxidase, an enzyme located almost exclusively at the outer mitochondrial membrane, toward the substrate phenylethylamine is used to report the oxygen pressure at the outer mitochondrial membrane of intact cardiac myocytes isolated from hearts of adult rats. The rate of substrate oxidation, under the conditions used, follows the Michaelis-Menten relation, and accordingly can be used as a measure of the local chemical activity of dissolved oxygen. The oxygen pressure at the outer mitochondrial membrane of myocytes, at rest and after 2- to 3-fold stimulation of respiratory oxygen consumption, differs from the extracellular oxygen pressure by at most 2 torr. This implies that most of the large, about 20 torr, difference in oxygen pressure between capillary lumen and mitochondria of the working heart must be extracellular. At physiologically relevant concentrations of the substrates phenylethylamine and norepinephrine, monoamine oxidase activity is relatively insensitive to extracellular oxygen pressure in the range 155 to 8 torr, suggesting a limited role for regulation of biogenic amine oxidation by oxygen availability.
mesh_terms: D000818:Animals; D007700:Kinetics; D008433:Mathematics; D008995:Monoamine Oxidase / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D009206:Myocardium / Q000166:cytology*; D010084:Oxidation-Reduction; D010100:Oxygen; D010101:Oxygen Consumption / Q000187:drug effects; D010627:Phenethylamines / Q000378:metabolism; D011312:Pressure; D051381:Rats
chemical_list: D008996:Monoamine Oxidase Inhibitors; D010627:Phenethylamines; C029261:phenethylamine; D008995:Monoamine Oxidase; D010100:Oxygen