An automated method for determination of the sedimentation coefficient of macromolecules using a preparative centrifuge.
Following centrifugation in a preparative ultracentrifuge at relatively high speeds for 1-3 h, quartz tubes containing approximately 150 microliter of macromolecular solution are optically scanned using an apparatus and method previously described (Attri, A. K., and Minton A. P. (1983) Anal. Biochem. 133, 142-152). The resulting data are processed by microcomputer immediately upon completion of scanning to yield the sedimentation coefficient of the solute. Calculated values agree to within a few percent with those found in the literature and with the results of control experiments carried out using an analytical ultracentrifuge.
D002919:Chymotrypsinogen / Q000032:analysis; D008433:Mathematics; D008838:Microcomputers; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013954:Thyroglobulin / Q000032:analysis; D014461:Ultracentrifugation / Q000379:methods*
D011506:Proteins; D013954:Thyroglobulin; D002919:Chymotrypsinogen