An interesting monoclonal anti-N produced following immunization with human group O, NN erythrocytes.
Spleen cells from Balb/c mice given multiple injections of intact human erythrocytes (group O, NN) were fused with NS1 myeloma cells. Culture fluids from the resulting hybrid cells were screened for agglutinating antibody against a panel of erythrocytes. One cell line, 2/23, secreted an IgM antibody which reacted more strongly with NN than with MM cells. Neuraminidase or papain treatment of erythrocytes abolished agglutination whereas trypsin treatment did not. Reactions with U- erythrocytes of different MN phenotypes confirmed the anti-N specificity of monoclonal antibody 2/23. This is the first report of monoclonal anti-N stimulated by the immunization of mice with intact erythrocytes.
D000017:ABO Blood-Group System / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004910:Erythrocyte Membrane / Q000276:immunology; D006801:Humans; D007114:Immunization; D008951:MNSs Blood-Group System / Q000276:immunology*
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D008951:MNSs Blood-Group System