Binding of disopyramide to alpha 1-acid glycoprotein in plasma measured by competitive equilibrium dialysis.
A modification of equilibrium dialysis in which alpha 1-acid glycoprotein and plasma compete directly for disopyramide has been used in conjunction with binding curves to measure the extent of the alpha 1-acid glycoprotein-disopyramide interaction. At concentrations in the therapeutic range, 80-90% of disopyramide was bound to alpha 1-acid glycoprotein for plasma from each of six healthy adults. Also, equilibrium dialysis data are presented, indicating that pH does not influence the binding of disopyramide within the therapeutic range.
D003956:Dialysis / Q000379:methods; D004206:Disopyramide / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009961:Orosomucoid / Q000378:metabolism*; D011485:Protein Binding
D009961:Orosomucoid; D004206:Disopyramide