Use of mini-octadecylsilane ultrasphere column in high-pressure liquid chromatography for protein structural studies.
Using a single mini-octadecylsilane (ODS) 5-micron ultrasphere column (0.46 X 4.5 cm) and linear gradients of different solvents, all the aspects of protein structural analysis have been defined. The effectiveness of the system has been evaluated by separating the alpha and beta chains of hemoglobin and their tryptic peptides, then performing amino acid analysis and, finally, identifying the phenylthiohydantoin derivatives of amino acids.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D006454:Hemoglobins / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification; D010669:Phenylthiohydantoin; D011506:Proteins / Q000032:analysis*; D012821:Silanes; D012825:Silicon; D013056:Spectrophotometry, Ultraviolet; D014357:Trypsin; D009764:o-Phthalaldehyde
D000596:Amino Acids; D006454:Hemoglobins; D010455:Peptides; D011506:Proteins; D012821:Silanes; C024779:octadecylsilane; D010669:Phenylthiohydantoin; D009764:o-Phthalaldehyde; D014357:Trypsin; D012825:Silicon