Dynamic laser light scattering of papain-treated thick filaments from limulus striated muscle in suspension.
Using quasielastic light scattering we have previously shown an increase in high frequency internal motion of isolated thick filament upon activation. This we have attributed to cross-bridge motion. Here we show that after cleavage of the S1 moiety of myosin from isolated filaments with papain, calcium ions no longer activate the isolated filaments to produce high-frequency motions.
D000818:Animals; D006737:Horseshoe Crabs; D066298:In Vitro Techniques; D007834:Lasers; D009119:Muscle Contraction / Q000187:drug effects*; D009132:Muscles / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D009210:Myofibrils / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D015879:Myosin Subfragments; D009218:Myosins / Q000378:metabolism; D010206:Papain / Q000494:pharmacology*; D010446:Peptide Fragments / Q000378:metabolism; D012542:Scattering, Radiation
D015879:Myosin Subfragments; D010446:Peptide Fragments; D010206:Papain; D009218:Myosins