Fast reversed-phase high-performance liquid chromatographic determination of nucleotides in red blood cells.
A method for the accurate determination of ATP, ADP, AMP, NADP and NAD in 20 min in red blood cell extracts by reversed-phase high-performance liquid chromatography is described. Experimental parameters affecting the separation are discussed.
D000244:Adenosine Diphosphate / Q000097:blood; D000249:Adenosine Monophosphate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004912:Erythrocytes / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009243:NAD / Q000097:blood; D009249:NADP / Q000097:blood; D009711:Nucleotides / Q000097:blood*; D013997:Time Factors
D009711:Nucleotides; D009243:NAD; D000249:Adenosine Monophosphate; D009249:NADP; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate