Alloimmunization induces an autoantibody reactive with a common, cryptic antigen.
C57BL/6 mice (haplotype H-2b) were alloimmunized by a classical protocol using repeated injections of DBA/2 spleen and liver cells (H-2d). The resulting polyclonal, hyperimmune antisera were highly cytotoxic to untreated and neuraminidase- (VCN)-treated DBA/2 spleen cells. An unexpected result was that the anti- H2d sera were also cytotoxic to VCN-treated C57BL/6 spleen cells. The cytotoxicity was limited to an IgM antibody and could be evoked by a single alloimmunization. The results demonstrate a new feature of the classical immune response, autoantibodies against a common, though cryptic murine antigen, designated Mo 1.
D000818:Animals; D000941:Antigens / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D006183:H-2 Antigens / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D008099:Liver / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009439:Neuraminidase / Q000494:pharmacology; D013154:Spleen / Q000276:immunology*
D000941:Antigens; D001323:Autoantibodies; D001324:Autoantigens; D006183:H-2 Antigens; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009439:Neuraminidase