The fusion of oocytes of the starfish Aphelasterias japonica. III. Reconstruction of oocytes from cells and cell fragments (cytoplasts).
The oocytes of the starfish Aphelasterias japonica were divided into anuclear (cytoplasts) and nuclear (germinal vesicles) fragments by centrifugation in a Ficoll gradient with cytochalasin B. Cell hybrids between the oocytes and the cytoplasts were then prepared with the aid of polyethylene glycol treatment. These cell hybrids were capable of responding to 1-methyladenine by maturation.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient / Q000379:methods; D005260:Female; D006822:Hybrid Cells / Q000502:physiology; D009865:Oocytes / Q000502:physiology* / Q000648:ultrastructure; D013215:Starfish / Q000502:physiology*