Discovery and initial characterization of a new conditional (temperature-sensitive) maternal effect mutation in the axolotl.
The discovery of a new temperature-sensitive maternal effect mutation in Ambystoma mexicanum is described. The new gene (ts-1) was recognized when 100% of the eggs spawned by homozygous females failed to develop past early gastrulation when reared at 25 degrees C. Eggs raised at 10 degrees C developed normally to sexual maturity. A temperature-sensitive period during blastulation was identified by a preliminary series of temperature shifts. Histologic examination revealed that nuclear abnormalities, especially chromosomal bridges, characterized ts-1 embryos reared beyond the ninth cleavage at the restrictive temperature (25 degrees C).
D000557:Ambystoma / Q000235:genetics*; D000558:Ambystoma mexicanum / Q000196:embryology / Q000235:genetics*; D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D009154:Mutation; D010641:Phenotype; D013696:Temperature