Further evidence that formation of the neural tube requires elongation of the nervous system.
In previous papers, we have correlated rapid elongation of the midline of the neural plate with the time of closure of the plate into a tube in the newt embryo and at one stage of the chick embryo. We proposed a model in which stretching of the midline of the plate causes the plate to buckle out of the plane and roll into a tube. In this paper, I show for another stage of development in the chick embryo, the period of closure of the brain tube, that rapid elongation of the nervous system accompanies closure of the tube. If elongation of the brain plate causes formation of the tube, then treatments that stop tube formation should also stop brain elongation. I tested this hypothesis by using low fluences of UV irradiation, known to stop tube formation (Davis, '44), and measuring the effects on elongation of the brain plate. The open plates of UV-irradiated embryos failed to elongate normally. Furthermore, photoreactivation with longer wavelengths of light reversed the UV effects and allowed closure of the tube in UV-irradiated embryos. These embryos elongated their brains.
D000818:Animals; D001699:Biometry; D001921:Brain / Q000196:embryology; D002490:Central Nervous System / Q000196:embryology* / Q000528:radiation effects; D002642:Chick Embryo / Q000254:growth & development* / Q000528:radiation effects; D009420:Nervous System / Q000196:embryology*; D014466:Ultraviolet Rays