Determination of D- and L-aspartate in amino acid mixtures by high-performance liquid chromatography after derivatization with a chiral adduct of o-phthaldialdehyde.
A sensitive and convenient method for the simultaneous determination of D- and L-aspartic acid in amino acid mixtures is described. The method involves derivatization of the mixture with a chiral fluorogen, followed by high-performance liquid chromatography on a reverse-phase column. The fluorogen used is an adduct of o-phthaldialdehyde with an optically active thiol, N-acetyl-L-cysteine. The sensitivity and accuracy of this method is similar to that using adducts of o-phthaldialdehyde with the achiral thiol, 2-mercaptoethanol. Five picomoles of D-aspartate can be accurately detected in the presence of a 100-fold excess of L-aspartate with a total analysis time (including derivatization) of 10 min.
D000111:Acetylcysteine; D000447:Aldehydes; D000596:Amino Acids / Q000032:analysis*; D001224:Aspartic Acid / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D005453:Fluorescence; D013237:Stereoisomerism; D009764:o-Phthalaldehyde
D000447:Aldehydes; D000596:Amino Acids; D001224:Aspartic Acid; D009764:o-Phthalaldehyde; D000111:Acetylcysteine