Characterization and localization of proopiomelanocortin messenger RNA in the adult rat testis.
Northern blot analysis of total RNA and polyadenylated RNA isolated from adult rat testes showed that a proopiomelanocortin (POMC)-like messenger RNA molecule is present in these extracts. The testicular POMC messenger RNA is comparable in length to amygdala and midbrain POMC messenger RNA and appears to be at least 200 nucleotides shorter than POMC messenger RNA found in the hypothalamus and anterior and intermediate lobes of the pituitary gland. Hybridization in situ showed that POMC messenger RNA is located in Leydig cells, which are the only testicular cells that contain immunostainable POMC-derived peptides. These results suggest that local synthesis of POMC occurs in the testis.
D000818:Animals; D001921:Brain / Q000378:metabolism; D007985:Leydig Cells / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D009693:Nucleic Acid Hybridization; D010902:Pituitary Gland / Q000378:metabolism; D010908:Pituitary Hormones, Anterior / Q000096:biosynthesis / Q000235:genetics*; D011333:Pro-Opiomelanocortin; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification*; D051381:Rats; D013737:Testis / Q000378:metabolism*; D014158:Transcription, Genetic
D010908:Pituitary Hormones, Anterior; D011498:Protein Precursors; D012333:RNA, Messenger; D011333:Pro-Opiomelanocortin