Synthesis of fibronectin in normal and osteoarthritic articular cartilage.
The content and the biosynthesis of fibronectin was examined in disease-free articular cartilage and in articular cartilage from osteoarthritic canine joints. Fibronectin content was increased in extracts of cartilage from osteoarthritic joints. Incubation of cartilage in vitro with [3H]phenylalanine and subsequent isolation of [3H]fibronectin from a gelatin affinity column and characterization by SDS-polyacrylamide gel electrophoresis and by immunoprecipitation indicated that disease-free and osteoarthritic cartilage explants synthesized fibronectin. About 50% of the [3H]fibronectin was recovered in the incubation medium. The osteoarthritic cartilage synthesized and accumulated up to 5-fold more [3H]fibronectin than disease-free cartilage.
D002358:Cartilage, Articular / Q000378:metabolism*; D005353:Fibronectins / Q000096:biosynthesis* / Q000302:isolation & purification; D006801:Humans; D007700:Kinetics; D010003:Osteoarthritis / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism; D014176:Protein Biosynthesis; D012016:Reference Values; D014316:Tritium
D005353:Fibronectins; D014316:Tritium; D010649:Phenylalanine