Expression of a distinct B cell clonotype profile after recovery from antigen-induced unresponsiveness.
The purpose of these experiments was to determine whether the B cell clonotype profile expressed in mice which have recovered from antigen-induced unresponsiveness is similar to that of nontolerized mice. Unresponsiveness to phosphorylcholine (PC) was initiated by injection of neonatal mice with PC-coupled human gamma globulin, resulting in the inability to respond to challenge with PC-lipopolysaccharide at 1.5 months of age. By 3 months of age, pretreated mice were 50% responsive while by 5 and 7.5 months of age, full responsiveness was observed. At each time point two differences distinguished the anti-PC antibody from nonpretreated mice. First, whereas nonpretreated mice displayed T15 dominance, pretreated mice did not. Second, the average avidity of T15-negative antibody produced in pretreated mice was greater than that in nonpretreated mice and similar to that of T15-positive antibody. Possible mechanisms for this "permanent" alteration of the antibody profile are discussed.
D000375:Aging; D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D000941:Antigens / Q000008:administration & dosage*; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology*; D002794:Choline / Q000031:analogs & derivatives*; D002999:Clone Cells / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007108:Immune Tolerance; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D010767:Phosphorylcholine / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D000941:Antigens; D008070:Lipopolysaccharides; D014301:Trinitrobenzenes; D010767:Phosphorylcholine; D002794:Choline