A human alloanti-N enhanced by acid media.
The serum from a 47-year-old man who had not been transfused previously was tested with reagent red cells selected from different commercial manufacturers to confirm the presence of an alloanti -N. Agglutination of homozygous N-positive red cells at 22 degrees C ranged from score 3 to 12. This variable reactivity was found to be due to pH, and the optimal reactivity was in test media below pH 7.
D000143:Acids; D001788:Blood Grouping and Crossmatching; D003298:Coombs Test; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007518:Isoantibodies / Q000032:analysis; D008951:MNSs Blood-Group System; D008297:Male; D008722:Methods; D008875:Middle Aged
D000143:Acids; D007518:Isoantibodies; D008951:MNSs Blood-Group System