title: Effect of different fractions of macromolecules upon triggering of calcium oxalate and calcium phosphate crystal formation in whole urine.
abstract: Macromolecules were removed from whole urine samples by sequential ultrafiltration using molecular weight cut-offs of 300,000, 100,000 and 10,000 dalton. The whole urine samples and ultrafiltrates were evaporated to 1,250 mosm/kg at pH 5.3 and 6.8 to study calcium oxalate and calcium phosphate crystal formation, respectively. While all the ultrafiltrates removed Tamm-Horsfall proteins and most of the crystal formation, there was some 10% of the crystal-promoting powers in the macromolecules in the fraction between molecular weight 100,000 and 300,000 dalton. This demonstrates the existence of a second crystal-promoting substance in the macromolecules of whole urine.
mesh_terms: D002129:Calcium Oxalate / Q000652:urine*; D002130:Calcium Phosphates / Q000652:urine*; D011232:Chemical Precipitation; D003460:Crystallization; D006801:Humans; D046911:Macromolecular Substances; D008970:Molecular Weight; D009088:Mucoproteins / Q000652:urine; D011507:Proteinuria / Q000652:urine; D014462:Ultrafiltration; D014556:Urine / Q000032:analysis*; D058949:Uromodulin
chemical_list: D002130:Calcium Phosphates; D046911:Macromolecular Substances; D009088:Mucoproteins; C548612:UMOD protein, human; D058949:Uromodulin; D002129:Calcium Oxalate