A high efficiency stirrer for suspension cell culture with or without microcarriers.
The stirrer described in this paper employs "Teaspoon stirring" in which a secondary motion is superimposed on the rotation of the liquid. This secondary motion arises from the viscous drag from the wall and bottom of the flask. The culture medium is rotated by a bulb-ended rod suspended from inside the flask cap: the bulb orbits around in a circular trough formed between a central indent in the base and the rounded periphery of the base. There are no bearings and no stagnant areas. The power used to stir 4 flasks each holding 500 ml of medium is less than 2 watts. The speed is electronically controlled by a tachometer and automatically gives a smooth start and stop as well as "Interval stirring" to assist initial attachment of cells.
D000818:Animals; D002478:Cells, Cultured; D046508:Culture Techniques / Q000295:instrumentation* / Q000379:methods; D013696:Temperature