[High-resolution 2-dimensional gel electrophoresis: a method for the clinical chemistry laboratory].
The working instructions, reagents and instruments for the performance of high resolution two dimensional gel electrophoresis are described. Size reduction of the gel slabs allows the two electrophoretic separation steps to be run within an 8-hour-working-day. The description also includes a well adapted method for silver staining and a photographic procedure for the documentation of the gels. The method is specially developed and designed for use in the clinical chemistry laboratory.
D002624:Chemistry, Clinical / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D007525:Isoelectric Focusing; D012967:Sodium Dodecyl Sulfate
D007074:Immunoglobulin G; D007202:Indicators and Reagents; D012967:Sodium Dodecyl Sulfate
Hochauflösende zweidimensionale Gelelektrophorese: Eine methode für das klinisch-chemische Laboratorium.