title: Prostaglandin synthesis in goldfish heart, Carassius auratus.
abstract: Potential precursors for prostaglandin (PG) synthesis were measured in goldfish heart and skeletal muscle by gas chromatography. Heart tissue contained docosahexaenoic, arachidonic, eicosapentaenoic, and eicosatrienoic acids in concentrations of 3223 +/- 128, 1216 +/- 7.8, 260 +/- 72.8, and 250 +/- 14 ng/mg wet wt, respectively. 14C-Labeled substrates were examined for their ability to be converted to prostaglandins. Eicosatrienoic and docosahexaenoic acid were not synthesized into prostaglandins, with 66 and 72% of the substrate remaining as free fatty acids, respectively. In contrast, both arachidonic and eicosapentaenoic acids were converted predominantly to PGFs and PGIs. The conversion was time dependent and complete by 30 min. The conversion patterns with eicosapentaenoic acid and arachidonic acid were essentially the same. The data suggest that goldfish cyclooxygenase can utilize two of the four potential substrates for prostaglandin synthesis. As fatty acid levels in fish vary with environmental temperature, substrate availability rather than cyclooxygenase preference may dictate the types of prostaglandins which are produced.
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D003530:Cyprinidae / Q000378:metabolism*; D006054:Goldfish / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D011453:Prostaglandins / Q000378:metabolism*
chemical_list: D011453:Prostaglandins