title: Carrageenan-induced suppression of chicken lymphocyte proliferation.
abstract: Mitogenic responsiveness of peripheral blood lymphocytes (PBL) of chickens was suppressed by either pretreatment with or addition to the culture medium of various concentrations of carrageenan (CGN). Pretreatment for 1 hr significantly suppressed response to Concanavalin A (Con A) and Pokeweed mitogen (PWM) but did not affect Phytohemagglutinin (PHA) induced stimulation. Extension of the pretreatment period to 4 hrs suppressed response induced by all three mitogens. On the other hand, addition of carrageenan to the culture medium caused a dose-dependent suppression of PHA and Con A mediated response, but the effect on stimulation due to PWM was equivocal. In addition, low concentrations of CGN were weakly mitogenic to PBL and splenic lymphocytes.
mesh_terms: D000818:Animals; D002351:Carrageenan / Q000494:pharmacology*; D002645:Chickens / Q000276:immunology; D003208:Concanavalin A / Q000037:antagonists & inhibitors; D004305:Dose-Response Relationship, Drug; D007165:Immunosuppression Therapy; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects; D010835:Phytohemagglutinins / Q000037:antagonists & inhibitors; D011043:Pokeweed Mitogens / Q000037:antagonists & inhibitors; D013997:Time Factors
chemical_list: D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D003208:Concanavalin A; D002351:Carrageenan