[Intracellular localization of fibronectin-synthesizing polyribosomes using I125 collagen].
Interaction of 125I-collagen with fibronectin-synthesizing polyribosomes, isolated from 9 days old chicken embryos, was studied. The interaction was maximal at the ratio of 0.1 microgram 125I-collagen and 5 ou of polyribosomes. Specific reaction of 125I-collagen with fibronectin polypeptides growing on polyribosomes was estimated by means of antibodies to fibronectin inhibiting the complex formation of polyribosomes-125I-collagen. The antibodies were shown to decrease the reaction rate of total polyribosomes (free and membrane-bound) with 125I-collagen by 25.4% and of free polyribosomes - by 6.7%. The data obtained suggest that fibronectin is synthesized mainly on polyribosomes, bound with the membranes of endoplasmic reticulum.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D002498:Centrifugation; D002642:Chick Embryo; D003094:Collagen / Q000378:metabolism*; D005353:Fibronectins / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D007457:Iodine Radioisotopes; D011132:Polyribosomes / Q000378:metabolism*
D000906:Antibodies; D005353:Fibronectins; D007457:Iodine Radioisotopes; D003094:Collagen
Izuchenie vnutrikletochnoĭ lokalizatsii fibronektinsinteziruiushchikh poliribosom pri pomoshchi [125I]kollagena.