Repair of DNA-protein cross-links induced by the mycotoxin botryodiplodin in mammalian cells.
Repair of DNA--protein cross-links (DPC) induced by the mycotoxin botryodiplodin in three cell strains (LF, VA and XP) was studied after removal of the drug and postincubation in fresh medium. Immediately after botryodiplodin was removed, DPC progressively disappeared with an efficiency which depended upon the dose of mycotoxin applied and the cell strain considered. Experiments using [14C]botryodiplodin suggested that DPC repair involved at least one splitting step, located between the DNA and the drug. Botryodiplodin also inhibited the replication process. After incubation of cells in fresh medium, DPC repair was not associated with concomitant resumption of DNA replication ability. Immediately after botryodiplodin removal, inhibition of DNA synthesis was strengthened for a few hours before DNA replication recovered. Possible mechanisms involved in the DPC repair process are discussed.
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D004260:DNA Repair / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005663:Furans / Q000633:toxicity*; D008297:Male; D009183:Mycotoxins / Q000633:toxicity*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D005663:Furans; D009183:Mycotoxins; D011506:Proteins; C006789:botryodiplodin; D004247:DNA