Effect of silver ions on transport and retention of phosphate by Escherichia coli.
Silver ions inhibited phosphate uptake and exchange in Escherichia coli and caused efflux of accumulated phosphate as well as of mannitol, succinate, glutamine, and proline. The effects of Ag+ were reversed by thiols and, to a lesser extent, by bromide. In the presence of N-ethylmaleimide and several uncouplers, Ag+ failed to cause phosphate efflux, but still inhibited exchange of intracellular and extracellular phosphate, indicating an interaction at more than one site. It is unlikely that Ag+ caused metabolite efflux by acting solely as an uncoupler, as an inhibitor of the respiratory chain, or as a thiol reagent.
D001692:Biological Transport / Q000187:drug effects; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D005033:Ethylmaleimide / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism*; D012835:Silver Nitrate / Q000494:pharmacology*; D013431:Sulfates / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D013696:Temperature; D014220:Trialkyltin Compounds / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology; D019287:Zinc Sulfate
D010710:Phosphates; D013431:Sulfates; D013438:Sulfhydryl Compounds; D014220:Trialkyltin Compounds; C011559:tributyltin; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D019287:Zinc Sulfate; D012835:Silver Nitrate; D015032:Zinc; D005033:Ethylmaleimide