Subendothelial deposition of von Willebrand's factor requires the presence of endothelial cells.
The presence of FVIII/VWF in human aortic subendothelium has been previously established. The present study was undertaken to determine the origin of this FVIII/VWF. Indirect immunofluorescence microscopy was employed to detect FVIII/VWF in rabbit aortas, after passage of a balloon catheter. Immediately after this de-endothelialization, FVIII/VWF was found to be present directly luminal to the internal elastic lamina. However, in the subsequent 5 days to 2 months, no FVIII/VWF was found on the luminal surface of exposed, nonre-endothelialized neointima. After endothelial cell regrowth, however, FVIII/VWF was again seen on the luminal surface. Thus these studies reveal that the deposition of subendothelial FVIII/VWF in neointima requires the presence of endothelial cells and that relatively trivial amounts of plasmatic FVIII/VWF are deposited on neointimal connective tissue after de-endothelialization.
D000818:Animals; D001011:Aorta / Q000502:physiology*; D001012:Aorta, Abdominal / Q000502:physiology; D001013:Aorta, Thoracic / Q000502:physiology; D001779:Blood Coagulation Factors / Q000502:physiology*; D004727:Endothelium / Q000502:physiology; D005455:Fluorescent Antibody Technique; D008297:Male; D008856:Microscopy, Fluorescence; D011817:Rabbits; D014841:von Willebrand Factor / Q000502:physiology*
D001779:Blood Coagulation Factors; D014841:von Willebrand Factor