Molecular cloning and regulation of expression of the genes for initiation factor 3 and two aminoacyl-tRNA synthetases.
A 22-kilobase fragment of the Escherichia coli chromosome which contains the genes for translation initiation factor 3, phenylalanyl-tRNA synthetase, and threonyl-tRNA synthetase was cloned into plasmid pACYC184. The hybrid plasmid (designated pID1) complements a temperature-sensitive pheS lesion in E. coli NP37. pID1-transformed NP37 overproduce initiation factor 3 and phenylalanyl-tRNA synthetase. Gene expression from pID1 was studied in vitro in a coupled transcription-translation system and in minicells. The results suggest that the genes for initiation factor 3 and phenylalanyl- and threonyl-tRNA synthetase are regulated by different mechanisms.
D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D010448:Peptide Initiation Factors / Q000096:biosynthesis / Q000235:genetics*; D010652:Phenylalanine-tRNA Ligase / Q000096:biosynthesis / Q000235:genetics; D010957:Plasmids; D039665:Prokaryotic Initiation Factor-3; D013914:Threonine-tRNA Ligase / Q000096:biosynthesis / Q000235:genetics
D010448:Peptide Initiation Factors; D039665:Prokaryotic Initiation Factor-3; D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase; D013914:Threonine-tRNA Ligase