Cell attachment and spreading on extracellular matrix-coated beads.
Parietal yolk sac cells M1536 -B3 grown on cytodex 2 beads deposited an extracellular matrix on the surface of the beads. Cell-free matrix-coated beads were isolated by treatment of the cell monolayer with cytochalasin B (CB) at a concentration of 10 micrograms/ml of phosphate-buffered saline (PBS). The matrix when analysed by electrophoresis on polyacrylamide gels (PAGE) revealed that the major components were laminin and entactin . The matrix-coated beads were used to study the attachment, spreading, and growth of African Green monkey BSC-40, human mammary MCF-7, mouse fibroblast L929, rat liver clone 9, and rat hepatoma H-4-II-E cells in defined serum-free growth medium. The different cell lines exhibited varying responses to matrix-coated vs uncoated beads with respect to rate of attachment, spreading, and growth. One of the most consistent responses observed was the enhancement of cell spreading on matrix-coated beads. The results suggested that the matrix-coated beads will provide a readily available and valuable tool for studies on cell surface-extracellular matrix interactions and the physiological consequences of those interactions.
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005109:Extracellular Matrix / Q000502:physiology*; D006801:Humans; D051379:Mice; D008855:Microscopy, Electron, Scanning; D051381:Rats; D013997:Time Factors