Affinity chromatography of estrogen receptors on diethylstilbestrol-agarose.
Diethylstilbestrol was coupled to epoxy-activated agarose yielding an affinity resin which is highly efficient for the isolation of estrogen receptors. This resin, diethylstilbestrol-agarose (DES-agarose), bound two proteins (Mr = 50,000 and 65,000) from rabbit uterine cytosol that show a specific interaction with estradiol. A two step procedure--adsorption on DES-agarose followed by a selective elution with p-sec-amylphenol and NaSCN, yielded highly purified estrogen receptors which can be used in the studies of estradiol-receptor interactions with other cell constituents.
D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods*; D003600:Cytosol / Q000032:analysis; D004054:Diethylstilbestrol; D004958:Estradiol; D005260:Female; D008970:Molecular Weight; D011817:Rabbits; D011960:Receptors, Estrogen / Q000302:isolation & purification*; D012685:Sepharose; D014599:Uterus / Q000032:analysis
D002352:Carrier Proteins; D011960:Receptors, Estrogen; D004958:Estradiol; D004054:Diethylstilbestrol; D012685:Sepharose