title: Cell death in the dorsal part of the chick optic cup. Evidence for a new necrotic area.
abstract: The spatiotemporal pattern of morphogenetic cell death during the early development of the chick retina was studied by means of the neutral red vital staining and light microscopy. A modification of the conventional procedure of vital staining, which consisted of the injection of the dye into the neural tube lumen, was used for this purpose. In addition to the two areas of cell death known from previous literature, the first located in the ventral part of the optic cup and the second located in the insertion of the optic stalk with the diencephalon, a new area of cell death was described. This third necrotic area was located in the protruding dorsal part of the optic cup rim and was present throughout the stages 15 to 18. The area consisted of dying cells, fragments and phagocytosed cells. We suggest that this dorsal area of cell death could stop the intense dorsal growth of the optic cup and/or reshape the optic cup rim. Moreover, this area may influence the production of cell degeneration in the dorsal part of the invaginating lens placode.
mesh_terms: D000818:Animals; D002470:Cell Survival; D002642:Chick Embryo / Q000166:cytology*; D009024:Morphogenesis; D009336:Necrosis; D009499:Neutral Red; D009898:Optic Disk / Q000196:embryology* / Q000473:pathology
chemical_list: D009499:Neutral Red