title: Possible misinterpretations of radioimmunoassay results of endocrine tissues containing peptides in the micromolar range.
abstract: This paper describes the possible inhibition of labelled peptidic antigen antibody bindings of high affinity (affinity constant above 10(11) l/mol) by structurally non-related synthetic peptides according to their primary aminoacid sequence at concentrations of only 10(-6) mol/l i.e. approximatively 10(-3) g/l, in two radioimmunoassays. Such concentrations of peptides are present in 10% weight/volume suspension of various neuroendocrine and endocrine fresh tissues and may therefore be responsible for false positive results.
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D004702:Endocrine Glands / Q000032:analysis*; D005755:Gastrins / Q000032:analysis / Q000276:immunology; D006801:Humans; D008832:Microchemistry; D010455:Peptides / Q000032:analysis* / Q000276:immunology; D011863:Radioimmunoassay; D051381:Rats
chemical_list: D005755:Gastrins; D010455:Peptides