[Rapid method for the simultaneous determination of bacterial urease and phenylalanine desaminase].
Tested was an accelerated method for the evaluation of urease and phenylalanine desaminase of a total of 327 strains of E. coli, S. typhimurium, S. enteritidis, S. braenderup, S. tennessee, P. vulgaris, P. mirabilis, Yersinia enterocolitica, and Citrobacter that were subjected to a preliminary identification with routine methods. It was established that the method tested had the following advantages as compared to the classical methods employed up to then in the laboratory practice: --100% positive results were obtained up to the 4th hour, i. e. the method appeared 6 times more rapid, and the overall result was obtained one day earlier; --the two enzymes were studied simultaneously; --the expenditures in connection with the use of nutrient medium per one test were several times lower. Suggested is the timely adoption of the accelerated method in the laboratory practice.
D000594:Amino Acid Oxidoreductases / Q000032:analysis*; D004755:Enterobacteriaceae / Q000145:classification / Q000201:enzymology*; D008722:Methods; D014510:Urease / Q000032:analysis*
D000594:Amino Acid Oxidoreductases; C022342:phenylalanine oxidase; D014510:Urease
Uskoren metod za ednovremenno opredeliane ureazata i fenilalanin dezaminazata na bakteriite.