Evidence for different patterns of post-translational processing of pro-enkephalin in the bovine adrenal, colon and striatum indicated by radioimmunoassay using region-specific antisera to Met-Enk-Arg6-Phe7 and Met-Enk-Arg6-Gly7-Leu8.
Specific antisera to two opioid peptides, Met-Enk-Arg6-Phe7 and Met-Enk-Arg6-Gly7-Leu8, were used in radioimmunoassay to study molecular forms in extracts of bovine adrenal, striatum and colon. The antisera to Met-Enk-Arg6-Phe7 and Met-Enk-Arg6-Gly7-Leu8 were each highly specific for the C-terminus of their respective haptens and showed less than 1% cross-reactivity with related enkephalins and their variants. In adrenal extracts, gel filtration on Sephadex G-50 indicated N-terminally extended forms of Met-Enk-Arg6-Phe7 and Met-Enk-Arg6-Gly7-Leu8 as the predominating immunoreactive species, whereas in the striatum and colon the main immunoreactive forms were compatible with the hepta- and octapeptides. The results suggest different patterns of post-translational processing of precursors in the adrenal medulla compared with striatal and colonic neurones.
D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D003106:Colon / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives / Q000032:analysis; D004745:Enkephalins / Q000378:metabolism*; D009928:Organ Specificity; D011498:Protein Precursors / Q000378:metabolism*; D011863:Radioimmunoassay
D004745:Enkephalins; D011498:Protein Precursors; C029992:proenkephalin; D004744:Enkephalin, Methionine; C027511:enkephalin-Met, Arg(6)-Phe(7)-; C033453:enkephalin-Met, Arg(6)-Gly(7)-Leu(8)-