title: Observations on two strains of plasmodium falciparum from Haiti in Aotus monkeys.
abstract: Two strains of Plasmodium falciparum originating in Haiti were studied in the Aotus monkey. The Haitian I/CDC strain was first adapted to in vitro cultivation and subsequently inoculated into monkeys. The Haitian III/CDC strain was inoculated directly from a human patient into the Aotus monkey. The strains varied in their levels of pathogenicity to the animals. The Haitian I/CDC strain was highly virulent in six splenectomized animals; in one intact animal, the infection could be controlled but not eliminated with periodic doses of quinine and chloroquine. After subsequent splenectomy, the animal developed high parasitemias and died. No gametocytes developed in any of the Haitian I infections. The Haitian III strain was lethal to five of the 14 splenectomized monkeys inoculated, but some were able to control their infections without drug intervention. Gametocytes developed in all infections that persisted for an adequate length of time, and infections of mosquitoes were obtained both during the primary attack and the first recrudescence of the parasitemia. Of the mosquitoes tested, Anopheles freeborni was most susceptible to infection, followed by An. culicifacies, An. dirus, An. maculatus, and An. albimanus. The Haitian III strain was successfully transmitted to four other splenectomized Aotus monkeys via sporozoite inoculation using An. freeborni.
mesh_terms: D000818:Animals; D000852:Anopheles / Q000469:parasitology; D001029:Aotus trivirgatus / Q000469:parasitology*; D001769:Blood / Q000469:parasitology; D002427:Cebidae / Q000469:parasitology*; D006205:Haiti; D008288:Malaria / Q000469:parasitology / Q000635:transmission / Q000662:veterinary*; D008992:Monkey Diseases / Q000469:parasitology* / Q000635:transmission; D010963:Plasmodium falciparum / Q000254:growth & development* / Q000472:pathogenicity; D013045:Species Specificity