The use of lectins for determination of absolute configurations of small amounts of sugars eluted from chromatograms.
A simple procedure for the determination of the absolute configuration (i.e., assignment to the D- or L-enantiomeric series) of glucose, mannose, galactose, fucose, arabinose, and rhamnose is described, based on inhibition by these sugars of 125I-labeled lectin binding to the glycoconjugates immobilized on the wells of plastic microculture plates. The method works well with 10 to 100-micrograms amounts of the sugars isolated after paper chromatography of the glycoprotein or polysaccharide hydrolysates.
D055598:Chemical Phenomena; D002621:Chemistry; D002854:Chromatography, Paper; D006023:Glycoproteins / Q000032:analysis; D037102:Lectins / Q000378:metabolism; D009005:Monosaccharides / Q000302:isolation & purification* / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis; D011137:Polystyrenes; D011869:Radioligand Assay / Q000379:methods
D006023:Glycoproteins; D037102:Lectins; D009005:Monosaccharides; D011134:Polysaccharides; D011137:Polystyrenes