Hypomethylation of hepatic nuclear DNA in rats fed with a carcinogenic methyl-deficient diet.
A progressive decrease was observed in the 5-methyldeoxycytidine content of hepatic DNA in male F344 rats fed with a hepatocarcinogenic methyl-deficient diet. The same dietary regimen resulted in altered hepatic contents of S-adenosylmethionine, the methyl-donating species, and S-adenosylhomocysteine, an inhibitor of DNA methylase. The data indicate that this carcinogenic dietary manipulation is sufficient to alter a possible regulatory process, DNA methylation.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002796:Choline Deficiency / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000032:analysis; D004032:Diet / Q000009:adverse effects; D004052:Diethylnitrosamine; D008099:Liver / Q000378:metabolism*; D008113:Liver Neoplasms / Q000209:etiology; D008297:Male; D008715:Methionine / Q000172:deficiency*; D008745:Methylation; D051381:Rats; D011916:Rats, Inbred F344; D012435:S-Adenosylhomocysteine / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000378:metabolism
D003841:Deoxycytidine; D004052:Diethylnitrosamine; D012436:S-Adenosylmethionine; D004247:DNA; D012435:S-Adenosylhomocysteine; D008715:Methionine; C016569:5-methyldeoxycytidine