Purification of quadrol for microsequencing in the spinning-cup sequenator.
Treatment of Quadrol buffer with isothiocyanatophenylthiocarbamylaminoethylaminopropyl glass (DITC glass) substantially reduces the impurities observed when this buffer is used with the spinning cup sequenator. Use of DITC glass-treated Quadrol buffer permits identification of PTH-amino acids from protein degradations down to 100 pmol.
D000595:Amino Acid Sequence; D001322:Autoanalysis / Q000295:instrumentation; D002021:Buffers / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods; D005029:Ethylenediamines / Q000302:isolation & purification*; D008832:Microchemistry
D002021:Buffers; D005029:Ethylenediamines; C010629:1,1',1'',1'''-(ethylenedinitrilo)tetra-2-propanol