Identification of multiple-resistance (R) and colicinogeny (Col) plasmids in an epidemic Salmonella agona serotype in Rio de Janeiro.
A Salmonella agona strain has caused a hospital outbreak of gastroenteritis in a pediatric unit in Rio de Janeiro. It bears two plasmids, a small (6.5 MDa molecular weight) plasmid coding for type B colicin production and a larger one (36 MDa molecular weight) determining resistance to ampicillin, gentamicin, kanamycin and trimethoprim-sulphamethoxazole. The R-plasmid, but not the Col-plasmid, is self-transferable to a Escherichia coli recipient strain. Curing for the R-plasmid was achieved by treatment with 0.05% SDS followed by incubation at 44 degrees C. It has not been possible to cure the S. agona strain for its Col-plasmid.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003086:Bacteriocin Plasmids; D001938:Brazil; D002648:Child; D003087:Colicins / Q000096:biosynthesis; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000302:isolation & purification; D004196:Disease Outbreaks; D006358:Hot Temperature; D006801:Humans; D010957:Plasmids; D011815:R Factors; D012475:Salmonella / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D012480:Salmonella Infections / Q000453:epidemiology / Q000382:microbiology*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014169:Transformation, Bacterial
D000900:Anti-Bacterial Agents; D003087:Colicins; D004269:DNA, Bacterial; D012967:Sodium Dodecyl Sulfate