title: Epididymal proteins mimic the androgenic effect on zona pellucida recognition by immature hamster spermatozoa.
abstract: The increase in zona pellucida binding caused by the exposure of cultured proximal corpus epididymidis to 2 microM-5 alpha-DHT (0.87 and 4.29 spermatozoa/egg for control and 5 alpha-DHT group respectively) was lost when 20 microM-cycloheximide was also added to the medium (0.72 spermatozoa/egg). These results were interpreted as meaning that de-novo protein synthesis was required to obtain the effect of androgens. When a fraction enriched in epididymal glycoproteins EP2-EP6 (18% total protein in epididymal cytosol and 30% in enriched fraction) and depleted of androgens (less than 120 pg testosterone + DHT/ml) was added to the cultured epididymal tubules, the zona pellucida-binding ability of the contained spermatozoa increased from 0.55 in controls to 2.73 spermatozoa/egg in the extract-treated group (P less than 0.02). When the enriched fraction was prepared from epididymides of 30-day castrates, the stimulatory effect was lost (1.04 spermatozoa/egg). We suggest that proteins synthesized in the epididymis are required to obtain the effect of androgens and that the glycoproteins EP2-EP6 may be involved.
mesh_terms: D000818:Animals; D002369:Castration; D006224:Cricetinae; D003513:Cycloheximide / Q000494:pharmacology; D013196:Dihydrotestosterone / Q000494:pharmacology; D004822:Epididymis / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006023:Glycoproteins / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D008647:Mesocricetus; D009924:Organ Culture Techniques; D010063:Ovum / Q000502:physiology*; D013079:Sperm Maturation / Q000187:drug effects*; D013084:Sperm-Ovum Interactions / Q000187:drug effects; D015044:Zona Pellucida / Q000502:physiology*
chemical_list: D006023:Glycoproteins; D013196:Dihydrotestosterone; D003513:Cycloheximide