Systematic investigation of enrichment media for wild-type Campylobacter jejuni strains.
Of the media examined, thioglycolate broth supplemented with 5% lysed sheep blood, Butzler antibiotic mixture, and 0.1% lauryl sulfate was the most sensitive enrichment medium for recovery of wild-type strains of Campylobacter jejuni from cecal contents of chickens and chicken livers. It allowed the retrieval of 1 CFU as did solid media but permitted the screening of 50-times larger volumes. Double-strength enrichment medium required 5 to 10 CFU for growth. Omission of lauryl sulfate reduced the sensitivity. Replacement of Butzler antibiotic mixture with Blaser antibiotic mixture increased overgrowth and, therefore, decreased retrieval of C. jejuni.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002168:Campylobacter fetus / Q000187:drug effects / Q000302:isolation & purification*; D002432:Cecum / Q000382:microbiology; D002645:Chickens; D003470:Culture Media; D005233:Fatty Alcohols / Q000494:pharmacology; D008099:Liver / Q000382:microbiology; D012967:Sodium Dodecyl Sulfate; D013864:Thioglycolates / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D003470:Culture Media; D005233:Fatty Alcohols; D013864:Thioglycolates; D012967:Sodium Dodecyl Sulfate; C028913:dodecyl sulfate