Leakiness of brush-border vesicles.
From the water content of pelleted brush-border vesicles and from a comparison of the aqueous volume within the pellet that is available to [3H]inulin (58%), inulin [14C]carboxylic acid (34%, both approx. 5000 daltons), [3H]raffinose (97%, 540 daltons) and [3H]glucose (94%, 180 daltons) it is concluded that only 1 in 4 to 6 of the brush-border vesicles is sealed. The implication of this finding for labelling and transport studies and for vesicle formation is discussed.
D000818:Animals; D001826:Body Fluids / Q000032:analysis*; D002250:Carbon Radioisotopes; D002264:Carboxylic Acids; D002463:Cell Membrane Permeability; D005947:Glucose / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism / Q000648:ultrastructure; D007424:Intracellular Fluid / Q000032:analysis*; D007444:Inulin; D007700:Kinetics; D008871:Microvilli / Q000378:metabolism / Q000648:ultrastructure*; D011817:Rabbits; D011887:Raffinose; D014316:Tritium
D002250:Carbon Radioisotopes; D002264:Carboxylic Acids; D014316:Tritium; D007444:Inulin; D005947:Glucose; D011887:Raffinose