In vitro interaction of opioid peptides with phospholipids. Formation and characterization of complexes.
Interaction of Leu- and Met-enkephalin with phosphatidylserine has been studied by chromatographic and spectrophotometric techniques. The main results of our investigation may be summarized as follows: i) Both enkephalins bind to phosphatidylserine; ii) no difference between the two enkephalins is noticeable; and iii) with both peptides the binding phenomenon leads to the formation of two complexes with a definite stoichiometry which are sterically much smaller than the original vesicles. On the basis of the submitted data, a tentative model of the newly formed complexes is provided.
D000215:Acylation; D002850:Chromatography, Gel; D004723:Endorphins / Q000378:metabolism*; D004743:Enkephalin, Leucine / Q000378:metabolism; D004744:Enkephalin, Methionine / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010718:Phosphatidylserines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D004723:Endorphins; D010718:Phosphatidylserines; D010743:Phospholipids; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine