Specificity analysis of monoclonal anti-DNA antibodies from B6-lpr/lpr mice.
The binding properties of B6-lpr/lpr anti-DNA monoclonal antibodies were characterized to evaluate the influence of genetic background on the diversity and specificity of lpr-induced autoantibody responses. Six anti-DNA antibodies were produced from fusions with B6-lpr/lpr mice, while another was obtained from a fusion with a B6-+/+ mouse immunized with lipopolysaccharide. Each antibody bound single-stranded DNA in preference to double-stranded DNA, with variation of over 300-fold in relative binding activities. In terms of binding to a panel of synthetic polynucleotides, each antibody exhibited a unique antigenic specificity. This binding, however, was not dependent on recognition of a unique base or sugar moiety, since individual antibodies bound polymers of dissimilar composition. These results suggest a diversity of binding reactions for B6-lpr/lpr anti-DNA antibodies, with a clonal repertoire similar to that of mice from autoimmune backgrounds.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology; D001666:Binding Sites, Antibody; D004277:DNA, Single-Stranded / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009841:Oligonucleotides / Q000276:immunology; D011119:Polynucleotides / Q000276:immunology
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D011119:Polynucleotides