Phospholipid metabolism in human platelets preserved at 22 degrees C: differential effects of storage on phospholipase A2- and C-mediated reactions.
The effects of preservation at 22 degrees C on phospholipid metabolism were studied in human platelets. Stimulation of fresh platelets with thrombin caused a rapid and transient rise of 1,2-diacylglycerol (DG) which was derived from phosphatidylinositol (PI) by its strictly specific phospholipase C. Lysophosphatidylcholine (lysoPC) and lysophosphatidylethanolamine (lysoPE) were also accumulated as a result of the action of phospholipase A2. No significant changes in phospholipid metabolism were detected in platelets preserved at 22 degrees C up to 6 hr. However, platelets stored for more than 12 hr showed (1) an accumulation of both lysoPC and lysoPE before thrombin activation, (2) a subsequent decrease in the formation of lysoPC and lysoPE after thrombin activation when compared to fresh platelets, (3) a threefold lower rate of liberation of arachidonic acid than fresh platelets after activation, and (4) a lower rate and extent of aggregation than fresh platelets. Nevertheless, the amount of 1,2-DG produced during preservation up to 48 hr was similar to that observed in fresh platelets. The results indicate that the markedly enhanced activity of phospholipase A2, but not phospholipase C, that occurs during platelet storage leads to the deterioration of aggregation and arachidonic acid liberation in response to thrombin.
D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D001793:Blood Preservation; D004075:Diglycerides / Q000097:blood; D006801:Humans; D008244:Lysophosphatidylcholines / Q000097:blood; D008246:Lysophospholipids; D010714:Phosphatidylethanolamines / Q000097:blood; D010740:Phospholipases / Q000097:blood*; D010741:Phospholipases A / Q000097:blood*; D054467:Phospholipases A2; D010743:Phospholipids / Q000097:blood*; D010738:Type C Phospholipases / Q000097:blood
C011439:1,2-diacylglycerol; D004075:Diglycerides; D008244:Lysophosphatidylcholines; D008246:Lysophospholipids; D010714:Phosphatidylethanolamines; D010743:Phospholipids; C008301:lysophosphatidylethanolamine; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D010738:Type C Phospholipases