Assembly of transferred normal spleen cells in situ at sites eliciting the delayed hypersensitivity reaction to tumor antigens in mice.
Cell assembly at sites eliciting the delayed hypersensitivity reaction (DHR) to tumor antigens was studied using 51Cr-labeled cells. C3H/He mice were strongly immunized with syngeneic MM46 tumors. The MM46 antigen fraction elicited tumor-specific DHR when injected into the footpad of MM46-immunized mice. The test mice were then injected iv with 51Cr-labeled spleen cells from normal mice, their feet were cut off, and radioactivity in the feet was counted in a gamma-scintillation counter as a measure of the assembly of 51Cr-spleen cells at the site of the antitumor DHR. Assembly of spleen cells persisted for 48 hr after injection of the antigen fraction. Local passive transfer of the DHR also induced assembly of spleen cells, indicating that it is a cell-mediated immune reaction. On the basis of these results, the role of cell movement in host defense systems against tumors is discussed.
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D002465:Cell Movement; D002860:Chromium Radioisotopes; D006968:Hypersensitivity, Delayed / Q000473:pathology*; D007114:Immunization; D007116:Immunization, Passive; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D013154:Spleen / Q000473:pathology*; D013997:Time Factors
D000951:Antigens, Neoplasm; D002860:Chromium Radioisotopes