Electron imaging of crotoxin complex thin crystal at 3.5 A.
Crotoxin complex forms thin crystals which are suitable for electron crystallographic analysis. We have used a 100 kV electron microscope equipped with a superconducting lens to image this crystal embedded in glucose. Optical diffraction analysis of the micrographs show unambiguously a structural resolution of 3.9 A which has not been obtained with the conventional microscope at room temperature. A density map with a nominal resolution of 3.5 A has been synthesized from these images by computer processing techniques.
D000818:Animals; D003201:Computers; D003435:Crotalid Venoms / Q000032:analysis; D003439:Crotoxin; D003461:Crystallography; D008854:Microscopy, Electron; D012911:Snakes
D003435:Crotalid Venoms; D003439:Crotoxin