title: Replication of mumps virus in murine cells.
abstract: Mumps virus replication was examined in various culture cells derived from mice. Eight of 16 lymphoid cell lines and 4 of 13 non-lymphoid cell lines supported the replication of Vero cell-adapted Enders strain (EY) of mumps virus. EY strain replicated more efficiently in lymphoid cell lines than in non-lymphoid ones. T cell preference, however, was not observed in this study. The growth kinetics of EY strain in high yield cell lines such as EL-4, L1210 and NS-18 cells were similar to that in Vero cells, while in low yield cell lines such as DK, C243 and 203 GL cells the growth patterns varied respectively. Nineteen kinds of primary culture cells of murine origin all proved not to be susceptible for EY strain, even when spleen cells were stimulated with lectins or allogeneic cells. Seven other strains of mumps virus were examined for their ability to replicate in EL-4, L1210 and L929 cells. Four and 6 strains replicated in EL-4 cells and L1210 cells respectively, although the growth patterns and yields varied in each virus-cell combinations. On the other hand, none of 7 strains showed sufficient replication in L929 cells.
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004622:Embryo, Mammalian; D007668:Kidney; D007942:Leukemia, Experimental / Q000382:microbiology; D008214:Lymphocytes / Q000382:microbiology*; D008223:Lymphoma / Q000382:microbiology; D051379:Mice; D008815:Mice, Inbred Strains; D009109:Mumps virus / Q000254:growth & development*; D009374:Neoplasms, Experimental / Q000382:microbiology*; D013045:Species Specificity; D014779:Virus Replication