title: Fibronectin-mediated binding of group A streptococci to human polymorphonuclear leukocytes.
abstract: Human plasma was shown to promote the attachment of an avirulent strain of Streptococcus pyogenes to human polymorphonuclear leukocytes. Removal of the fibronectin by absorption of the plasma with agarose-immobilized gelatin abolished the attachment-promoting activity. The activity of the absorbed plasma was restored with purified human serum fibronectin. The fibronectin was found to promote the attachment of three M protein-negative, but not of three M protein-positive, strains of streptococci. Radiolabeled fibronectin was shown to bind in various degrees to all strains of S. pyogenes tested. Thus, in the absence of streptococci surface M protein, fibronectin promotes the association of group A streptococci with human polymorphonuclear leukocytes.
mesh_terms: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000276:immunology; D001665:Binding Sites; D001770:Blood Bactericidal Activity; D002352:Carrier Proteins; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D009504:Neutrophils / Q000378:metabolism*; D010587:Phagocytosis; D013297:Streptococcus pyogenes / Q000145:classification / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D005353:Fibronectins; C014689:streptococcal M protein