A modified assay system for enzymes involved in N-acetyl group transfer reactions: its use to study enzymes involved in ornithine biosynthesis in plants.
A new method for assaying the enzymes N2-acetyl-L-ornithine:L-glutamate N-acetyltransferase (EC 2.3.1.35) and acetyl-coenzyme A:L-glutamate N-acetyltransferase (EC 2.3.1.1) has been designed. This assay system is based on the separation of N-[14C]acetylglutamate from N-[14C]acetylornithine or [14C]acetyl-coenzyme A by differential absorption of these compounds to DEAE anion-exchange disks. The procedure is simple and sensitive and eliminates the use of ion-exchange resin columns. Using the present method, the inhibition of the enzyme N2-acetyl-L-ornithine:L-glutamate N-acetyltransferase by alpha-methylornithine and N-bromoacetylornithine was studied.
D000107:Acetylation; D000123:Acetyltransferases / Q000032:analysis* / Q000037:antagonists & inhibitors; D051046:Amino-Acid N-Acetyltransferase; D007887:Fabaceae / Q000201:enzymology*; D007475:Ion Exchange Resins; D009952:Ornithine / Q000096:biosynthesis*; D010940:Plant Proteins / Q000032:analysis; D010946:Plants, Medicinal
D007475:Ion Exchange Resins; D010940:Plant Proteins; D009952:Ornithine; D000123:Acetyltransferases; D051046:Amino-Acid N-Acetyltransferase; C020646:glutamate acetyltransferase