The separation of bovine prothrombin and descarboxyprothrombin by high-performance liquid chromatography.
Prothrombin contains 10 gamma-carboxyglutamic acid (Gla) residues which are absent in the warfarin-induced descarboxyprothrombin; hence prothrombin has 10 more negative groups than has descarboxyprothrombin. The two proteins can be separated by HPLC with the aid of an anion-exchange column. Plasma from warfarin-treated animals could be analyzed without pretreatment of the samples and a full analysis was obtained in 30 min.
D000818:Animals; D015415:Biomarkers; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D008722:Methods; D011498:Protein Precursors; D011516:Prothrombin / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D013917:Thrombin / Q000032:analysis; D013997:Time Factors; D014859:Warfarin / Q000097:blood
D015415:Biomarkers; D011498:Protein Precursors; C008208:acarboxyprothrombin; D014859:Warfarin; D011516:Prothrombin; D013917:Thrombin