Diazo-based assay for total bilirubin in a coated thin film evaluated.
We compared results for total bilirubin as measured on a coated thin film and by the Evelyn-Malloy and Jendrassik-Gróf methods. We examined serum samples from patients and studied the effects of protein, hemoglobin, and lipids on bilirubin measurement. Results from the thin-film assay compared favorably with those of the other methods. Total and within-day precision (CV), assessed over a one-year period, were better than 6% and 3%, respectively, at all concentrations. Analytical recovery was 99 +/- 3%. Samples from individuals having a wide range of liver diseases demonstrated, by linear regression, good correlation between the thin-film method and the two wet-chemistry methods (correlation coefficients of 0.990 and 0.994). We conclude that the thin-film method offers a valid alternative assay for total bilirubin.
D000328:Adult; D001322:Autoanalysis; D001663:Bilirubin / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis; D002914:Chylomicrons / Q000097:blood; D003951:Diagnostic Errors; D003979:Diazonium Compounds; D005069:Evaluation Studies as Topic; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007202:Indicators and Reagents; D007231:Infant, Newborn; D013053:Spectrophotometry / Q000379:methods; D013425:Sulfanilic Acids
D001798:Blood Proteins; D002914:Chylomicrons; D003979:Diazonium Compounds; D006454:Hemoglobins; D007202:Indicators and Reagents; D013425:Sulfanilic Acids; D001663:Bilirubin; C000469:diazobenzenesulfonic acid