Labelling of the integral proteins of sarcoplasmic-reticulum membranes.
Photoreactive lipids were introduced into rabbit and rat sarcoplasmic-reticulum membranes to label specifically integral proteins. Evidence was obtained that, in addition to the Ca2+-dependent ATPase, the 160 000-Mr glycoprotein, the 53 000-56 000-Mr components and polypeptides of Mr 30 000, 20 000 and 6000 are integral components of sarcoplasmic-reticulum membranes.
D000818:Animals; D001386:Azides; D004591:Electrophoresis, Polyacrylamide Gel; D005944:Glucosamine / Q000031:analogs & derivatives; D007425:Intracellular Membranes / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis*; D010713:Phosphatidylcholines; D011817:Rabbits; D051381:Rats; D012519:Sarcoplasmic Reticulum / Q000032:analysis*
D001386:Azides; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D005944:Glucosamine