Interference of O.C.T. embedding compound with hormone receptor assays.
The interference of O.C.T. (Optimum Cutting Temperature) embedding compound with the determination of estrogen and progesterone receptor analysis in rabbit uterus cytosol and human breast cancer tissue was investigated. It was determined that O.C.T. Compound reduces binding in rabbit uterus cytosol at concentrations greater than 1%. Hormone receptor binding greatly was reduced in human breast cancer tissues from O.C.T. embedded tissues when compared with the untreated tissue of the same patients. Progesterone receptor binding was affected more strongly than estrogen binding.
D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism; D005260:Female; D005404:Fixatives / Q000009:adverse effects*; D005629:Frozen Sections; D006801:Humans; D008867:Microtomy; D011247:Pregnancy; D011817:Rabbits; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*; D014599:Uterus / Q000378:metabolism
D005404:Fixatives; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone