Muscarinic cholinergic receptors on skin fibroblasts in familial affective disorder.
Cultured human skin fibroblasts possess muscarinic receptors with the properties of specific binding, saturability, pharmacologic specificity, inhibition of norepinephrine-stimulated adenylate cyclase, and increased binding after incubation with an antagonist. The number of binding sites appears to be a stable characteristic of each cell line. We studied fibroblasts from 18 patients with a major affective disorder and found that they had a higher density of binding sites than cells from 12 normal controls. Fibroblasts from 18 relatives who had histories of major or minor affective disorder also had a higher density, and those from five normal relatives were similar to controls. These results, although still preliminary, suggest that increased cholinergic-receptor density may be associated with vulnerability to affective disorders in some familial cases.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000341:Affective Disorders, Psychotic / Q000378:metabolism; D001714:Bipolar Disorder / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000032:analysis*; D012867:Skin / Q000032:analysis*
D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate; D000262:Adenylyl Cyclases