Liquid chromatographic assay of heptaminol in serum and its oral pharmacokinetics in the dog.
In order to investigate the pharmacokinetics of heptaminol in dogs, a high-performance liquid chromatographic assay of the drug was devised and it was evaluated in a general purpose validation design through analysis of variance. Heptaminol and its internal standard n-propylamine were salted out from plasma together with acetonitrile, the previously proposed "solvent demixing " extraction procedure. Both amines were derivatised in acetonitrile with the o-phthaldialdehyde, 2-mercaptoethanol procedure of Roth. The adducts were quantitated by reversed-phase high-performance liquid chromatography on Radial-Pak cartridges with ultraviolet detection. Peak height ratios were linearly related to concentrations up to 250 mumol l-1 with a 2% coefficient of variation. Sensitivity was 3.5 mumol l-1 (signal-to-noise ratio of 5). Means of the usual pharmacokinetic parameters in four dogs were: elimination half-life 3.75 h, apparent distribution volume 2.18 l kg-1 and total clearance 0.402 l kg-1 h-1, similar to the results obtained in humans by other authors using radiolabelled heptaminol .
D000284:Administration, Oral; D000605:Amino Alcohols / Q000097:blood*; D000704:Analysis of Variance; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D006535:Heptaminol / Q000008:administration & dosage / Q000097:blood*; D007700:Kinetics; D008433:Mathematics; D008657:Metabolic Clearance Rate
D000605:Amino Alcohols; D006535:Heptaminol