Detection of T-2 toxin in Fusarium sporotrichioides-infected corn by enzyme-linked immunosorbent assay.
A competitive enzyme-linked immunosorbent assay was used to screen for T-2 toxin in Fusarium sporotrichioides -infected corn. The assay detected T-2 toxin in diluted methanol extracts of corn samples at concentrations of 0.05 ng/ml. In infected corn samples, enzyme-linked immunosorbent assay and gas-liquid chromatography estimations of T-2 toxin concentrations were similar.
D002849:Chromatography, Gas; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005516:Food Microbiology; D005670:Fusarium; D000432:Methanol; D010936:Plant Extracts / Q000032:analysis; D012044:Regression Analysis; D012717:Sesquiterpenes / Q000032:analysis*; D013605:T-2 Toxin / Q000032:analysis* / Q000276:immunology; D014255:Trichothecenes / Q000032:analysis / Q000276:immunology; D003313:Zea mays / Q000032:analysis* / Q000382:microbiology
D010936:Plant Extracts; D012717:Sesquiterpenes; D014255:Trichothecenes; D013605:T-2 Toxin; D000432:Methanol