title: Detection of leukotriene C4-liked immunoreactivity in tear fluid from subjects challenged with specific allergen.
abstract: Tear fluid was obtained from allergic subjects from control eyes and eyes challenged with specific allergen and levels of leukotriene C4 (LTC4)-immunoreactivity determined by radioimmunoassay. Formal identification of the leukotrienes released was not possible but the levels of LTC4-immunoreactive material in allergen-challenged tear fluid (4.9 +/- 2.3 ng/ml, n = 9) were significantly higher (p less than 0.01) than those in control tear fluid (0.07 +/- 0.06 ng/ml, n = 9). These results provide evidence that leukotrienes, which account for the biological activity of slow reacting substance of anaphylaxis, may be released in allergic reactions in vivo in man.
mesh_terms: D000328:Adult; D000368:Aged; D000485:Allergens; D003429:Cross Reactions; D005260:Female; D006801:Humans; D007106:Immune Sera; D008297:Male; D008875:Middle Aged; D006109:Poaceae; D011863:Radioimmunoassay / Q000379:methods; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology*; D013189:SRS-A / Q000032:analysis*; D013666:Tears / Q000032:analysis* / Q000276:immunology
chemical_list: D000485:Allergens; D007106:Immune Sera; D013189:SRS-A