Effect of plane of nutrition on adipose tissue lipid metabolism in genetically obese and lean pigs.
Genetically lean and obese pigs of 27 kg body weight were individually fed at varying levels of intake. During the first 5 weeks (period 1) the pigs on the high plane nutrition (H) gained 19 kg, those on the medium plane (M) gained 7 kg and those on the low plane (L) lost 5 kg. During the second 5 weeks (period 2) the M pigs gained an additional 7 kg (MM), whereas the period 1 H pigs were fed to lose 5 kg (HL) and the period 1 L pigs were fed to gain 19 kg (LH). All pigs were targeted to weigh 41 kg at the end of 10 weeks. Subcutaneous adipose tissue samples were obtained by biopsy from each pig on day 28 and day 63 of the experiment, i.e., after 4 weeks of feeding a particular level of intake in each period. In vitro glucose metabolism to CO2, total lipids and glyceride fatty acids as well as basal and epinephrine-stimulated lipolytic rates were assessed. Obese pigs had greater cell size and lipogenic and lipolytic rates than lean pigs. During both periods the glucose metabolism rates paralleled the plane of nutrition, i.e., the low intake level yielded low rates and the high intake level yielded high rates. The lipolytic activities were refractory to the plane of nutrition. Finally the less muscular, obese and more muscular, lean pigs presented similar qualitative metabolic responses to their planes of nutrition, although the quantitative responses were divergent.
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D004032:Diet; D002149:Energy Intake; D004837:Epinephrine / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D050356:Lipid Metabolism; D008066:Lipolysis / Q000187:drug effects; D009765:Obesity / Q000235:genetics / Q000378:metabolism*; D013552:Swine / Q000378:metabolism*
D005947:Glucose; D004837:Epinephrine