Inhibition of gastric acid secretion by a phorbol ester: effect of 12-O-tetradecanoylphorbol-13-acetate on aminopyrine accumulation by rat parietal cells.
12-O-tetradecanoylphorbol-13-acetate ( 32nM ) inhibits aminopyrine accumulation induced by dibutyryl cyclic AMP or by histamine plus 3-isobutyl-1-methylxanthine in isolated rat parietal cells, but a significant effect on carbachol-stimulated aminopyrine accumulation is only seen at higher concentrations of the phorbol ester. 12-O-tetradecanoylphorbol-13-acetate ( 32nM ) does not affect aminopyrine accumulation or glucose oxidation in unstimulated cells, nor does it alter the ability of cells to exclude trypan blue. The phorbol ester may trigger a specific, and novel, parietal cell system capable of modulating the acid secretory response to histamine.
D000632:Aminopyrine / Q000378:metabolism*; D000818:Animals; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006633:Histamine Antagonists; D066298:In Vitro Techniques; D008297:Male; D010704:Phorbols / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D006633:Histamine Antagonists; D010704:Phorbols; D000632:Aminopyrine; D013755:Tetradecanoylphorbol Acetate