title: Modulation of types I and III procollagen synthesis at various stages of arterial smooth muscle cell growth in vitro.
abstract: Collagen synthesis was monitored in cultures of rabbit arterial smooth muscle cells (SMC). Both the rate of collagen synthesis per cell and collagen synthesis as a percent of total protein synthesis were measured at specific intervals from 1 to 14 days after inoculation of smooth muscle cells. The proportions of types I and III collagen present in the conditioned incubation medium and in the cell layer were also examined. After inoculation the cells displayed population expansion typical of SMC in which growth slowed but did not cease after the cells attained confluence. Collagen synthesis rates, expressed as [14C]hydroxyproline per cell, were eight-fold higher in preconfluent cells. In these cultures collagen accounted for more than 20% of the newly synthesized, 14C-labeled protein present as trichloroacetic acid (TCA)-insoluble material in 24 h culture media. In post-confluent cultures, this percentage was reduced to about 7% of the total protein synthesized. Synthesis rates of both collagen and non-collagen protein decreased with increasing time after inoculation. However, the rate of decline of collagen synthesis was three times greater than that seen for non-collagen protein. Early cultures synthesized relatively more type I than type III procollagen. The type I to type III ratio was highest at day 3 and declined after that time to day 14. While the synthesis of both types decreased with increasing age, type I declined at a greater rate resulting in a predominance of type III procollagen secretion by older cultures. We conclude that protein synthesis in general and collagen synthesis in particular are quantitatively and qualitatively dependent upon the growth stage of SMC in vitro.
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000502:physiology; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D007700:Kinetics; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000502:physiology*; D011347:Procollagen / Q000096:biosynthesis*; D011392:Proline / Q000378:metabolism; D014176:Protein Biosynthesis; D011817:Rabbits
chemical_list: D002250:Carbon Radioisotopes; D011347:Procollagen; D003094:Collagen; D011392:Proline