[Action of ketotifen on the mitogen-evoked proliferative response of human mononuclear cells].
Ketotifen at low concentrations (5 and 50 microns) potentiated and at high ones (250 and 500 microM) blocked the proliferative response of human peripheral blood mononuclear cells ( NNC ) induced by PHA. The proliferative response was evaluated by 3H-thymidine incorporation into the cells. At doses inhibiting proliferative response to PHA ketotifen blocked both Con A-induced and Pokeweed mitogen-induced proliferations of MNC. At tested concentrations ketotifen inhibited and at the highest concentration (250 microM) blocked PHA-induced increase of protein synthesis in MNC evaluated by incorporation of 14C-L-leucine into the cells. The presented data showed that ketotifen acted not on the inductive step of the proliferative response but on the steps preceding the cell shift into S-phase.
D000328:Adult; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007665:Ketotifen / Q000494:pharmacology*; D008297:Male; D008934:Mitogens / Q000494:pharmacology*; D009000:Monocytes / Q000166:cytology / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013997:Time Factors
D008934:Mitogens; D010835:Phytohemagglutinins; D003208:Concanavalin A; D007665:Ketotifen
Deĭstvie ketotifena na vyzvannyĭ mitogenami proliferativnyĭ otvet mononuklearnykh kletok cheloveka.