Application of sodium stannite in the preparation of instant [99mTc]HIDA kit.
A rapid method for the production of freeze-dried kit containing 20 mg HIDA and 0.4 mg SnCl2 X 2H2O at pH 5.5-6.0 was developed using the stannite procedure. The Sn-HIDA ligand has been prepared successfully by dissolving HIDA in stannite solution without further pH adjustment. A gelchromatography column scanning (GCS) technique has been applied to study the chemical state of technetium in the preparation. The shelf-life of the freeze-dried powder of Sn-HIDA was found stable for at least 6 months as assessed by the GCS-technique. The hepatobiliary agent has a rapid blood clearance and is excreted predominantly by the hepatobiliary system. The biodistribution study in mice showed that more than 30% of the injected dose has been accumulated in liver at 5 min postinjection and at 30 min most of the injected activity has been passed to the intestine via the biliary system.
D000818:Animals; D004355:Drug Stability; D007098:Imino Acids / Q000097:blood / Q000378:metabolism; D007202:Indicators and Reagents; D007553:Isotope Labeling; D051379:Mice; D011817:Rabbits; D011933:Reagent Kits, Diagnostic; D013667:Technetium / Q000097:blood / Q000378:metabolism; D019780:Technetium Tc 99m Lidofenin; D014001:Tin; D017971:Tin Compounds; D014018:Tissue Distribution
D007098:Imino Acids; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic; D017971:Tin Compounds; C041933:stannite; D019780:Technetium Tc 99m Lidofenin; D013667:Technetium; D014001:Tin