title: Effects of colchicine on the formation and looping of the tubular heart of the embryonic chick.
abstract: The role of microtubules in the early development of the chick embryo heart was studied. The microtubules were disrupted by treatment of the embryos with colchicine. The embryos were divided for study into three groups: (I) before the fusion of the paired cardiac primordia; (II) before the starting of the cardiac loop, and (III) during the formation of the looping process. Colchicine did not impair the fusion of the paired heart primordia nor the acquisition of an asymmetric C-shaped form. However, the normal counterclockwise movement of the heart loop was prevented. From these results we conclude that the formation of the tubular heart and its looping are independent of the integrity of the microtubular system. Under the effects of colchicine the developing myocytes rounded up bulging into the pericardial cavity. The cell contours became scarcely discernible and the individual cell surfaces gave rise to blebs and ruffles of different sizes. In older embryos, clefts of different sizes appeared between the myocardial cells. The effects of colchicine on the pulsatile activity of the heart were recorded. These effects, as well as those on the cell surface characteristics, were found to be age dependent. The more mature the hearts were, the more resistant to colchicine they became. The developmental significance of the results reported here is discussed.
mesh_terms: D000818:Animals; D002642:Chick Embryo / Q000254:growth & development*; D003078:Colchicine / Q000494:pharmacology*; D006321:Heart / Q000196:embryology*; D008855:Microscopy, Electron, Scanning; D008870:Microtubules / Q000502:physiology; D009206:Myocardium / Q000166:cytology / Q000648:ultrastructure; D011674:Pulse / Q000187:drug effects
chemical_list: D003078:Colchicine