Inhibition of synaptosomal 5-[3H]hydroxytryptamine uptake by endogenous factor(s) in human blood.
The inhibition of 5-[3H]hydroxytryptamine uptake into rat forebrain synaptosomes by human plasma extracts was studied. Highly potent, small (Mr less than 10 000), and heat stable factor(s) were found to inhibit 5-[3H]hydroxytryptamine uptake specifically, reversibly and in a non-competitive manner. The possible role of these factor(s) as endogenous modulators of serotonergic activity is discussed.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D001769:Blood; D001921:Brain / Q000378:metabolism; D006358:Hot Temperature; D007099:Imipramine / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism*
D012701:Serotonin; D007099:Imipramine