[Studies on pathogenesis of muscular dystrophy: levels of thiobarbituric acid-reactive products in avian muscular dystrophy].
Lipid peroxidation and other free radical reactions are known to disrupt and damage cellular structures and function, and it has been postulated as possible mechanisms of cellular damage of muscular dystrophy because increased levels of thiobarbituric acid (TBA)-reactive products and increased activities of superoxide dismutase and glutathione peroxidase were reported in avian muscular dystrophy. We reported that activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase were significantly increased in avian muscular dystrophy from the early developmental stage. Since these enzymes protect cellular structures from free radicals and peroxides, increased activities of these enzymes would indicate increased formation of radicals. Then it seems to be important to assay TBA-reactive products which indicate tissue malondialdehyde content, a by-product of lipid peroxidation. We used dystrophic chickens of New Hampshire series line 413 and their controls line 412 for assay of TBA-reactive products. Four or five birds from respective lines were killed by decapitation two weeks, four weeks and four months after hatching. The superficial pectoral muscle was immediately weighed and levels of TBA-reactive products in the muscle homogenate was assayed by fluorophotometry according to the modified method of Ohkawa and Tanizawa. Levels of TBA-reactive products were significantly higher in dystrophic chickens at all stages of development studied than those of the control group. At two weeks of age morphological changes are minimum if present and increased levels of TBA-reactive products cannot be considered as a secondary change of morphological alterations. Therefore, the results indicate involvement of lipid peroxidation damage in pathogenesis of this avian muscular dystrophy.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002645:Chickens; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D009137:Muscular Dystrophy, Animal / Q000201:enzymology / Q000209:etiology* / Q000378:metabolism; D010369:Pectoralis Muscles / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013858:Thiobarbiturates / Q000032:analysis*
D013858:Thiobarbiturates; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; C029684:thiobarbituric acid