The effects of naloxone, morphine and methionine enkephalinamide on Ia afferent terminations in the cat spinal cord.
Naloxone, morphine, Met5-enkephalinamide (MENKA) and procaine were administered microelectrophoretically near extracellularly stimulated extensor muscle group Ia afferent fibres and terminations in the lumbar spinal cord of cats anaesthetized with pentobarbitone sodium. Observations were made of effects on the electrical threshold, on the depolarizing action of GABA or piperidine-4-sulphonate (P4S), and on bicuculline-sensitive primary afferent depolarization (PAD) generated by tetanic stimulation of flexor muscle low threshold afferents. All 4 agents reversibly elevated the threshold of Ia fibres in the dorsal column and Ia terminations in the ventral horn. The depolarizations of terminations by GABA or P4S were also reduced, an effect, which for all except MENKA, probably accounted for a concomitant reduction in PAD. In the absence of a consistent effect on either threshold or depolarization by GABAmimetics, MENKA reversibly diminished PAD, an action blocked by naloxone. Intravenously administered naloxone, in doses known to enhance spinal monosynaptic excitation in the cat, had no effect on GABAergic PAD and little or no effect on Ia termination threshold. The results are discussed in relation to a naloxone-sensitive effect of MENKA which reduces transmitter release from GABAergic axo-axonic synapses on Ia terminals, but which does not account for the enhancement of spinal reflexes by naloxone.
D000344:Afferent Pathways / Q000187:drug effects; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D002415:Cats; D004558:Electric Stimulation; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009020:Morphine / Q000494:pharmacology*; D009132:Muscles / Q000294:innervation*; D009270:Naloxone / Q000494:pharmacology*; D010880:Piperidines / Q000494:pharmacology; D011343:Procaine / Q000494:pharmacology; D012023:Reflex, Monosynaptic / Q000187:drug effects; D013569:Synapses / Q000187:drug effects; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D010880:Piperidines; D009270:Naloxone; D011343:Procaine; D005680:gamma-Aminobutyric Acid; D004744:Enkephalin, Methionine; C021055:Met-enkephalinamide; C023402:piperidine-4-sulfonic acid; D009020:Morphine; D001640:Bicuculline