Protein methylation in normal and cystic fibrosis fibroblasts.
Human-cultured fibroblasts contain protein methylase activities. These activities were determined and the enzymatic products were identified after acid hydrolysis of the protein substrate for protein methylases I (arginine) and III (lysine) and by organic solvent extraction of the methanol produced by alkaline treatment of the protein substrate (for the protein methylase II). A methylation of histidine residues of proteins occurs in cultured fibroblasts. Protein methylase activities were unmodified in the cystic fibrosis fibroblasts as compared to the control cells.
D002118:Calcium / Q000502:physiology; D002147:Calmodulin / Q000502:physiology; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000378:metabolism*; D006801:Humans; D008745:Methylation; D011496:Protein Methyltransferases / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000378:metabolism*
D002147:Calmodulin; D011506:Proteins; D011496:Protein Methyltransferases; D002118:Calcium