title: Assessment of smooth muscle function in Sesbania drummondii toxicosis in Gallus domesticus.
abstract: An in vitro assessment based on tissue responsiveness to 2 agonists-histamine and carbachol-was made on smooth muscle activity in chickens experimentally poisoned with sesbania. Crude extracts of Sesbania drummondii were prepared and 2 dosage levels, 0.25% and 0.5% of body weight, were used. The birds were dosed orally with the extract for 3 consecutive days, and on the 4th day segments of ileum and lung were collected from each bird. The isometric contractions of each tissue, produced by the addition of histamine or carbachol in graded concentrations, were recorded. The cumulative concentration-effect curves for the tissues to the agonists were constructed and compared with respective control curves. The results indicated the responsiveness of the tissues in the treated groups was significantly decreased, compared with that of tissues in the controls. Responses of both intestinal and parenchymal strips in the chickens given the higher dosage (0.5%) were decreased significantly, whereas in those given the smaller dosage (0.25%), only parenchyma had a significant response. This indicates that the activity of smooth muscles in general was depressed by sesbania. The effect was more evident in the lung than in the intestine. Therefore, an active principle in the extract which affects smooth muscle, rather than causing direct irritation, may exist. This assessment of smooth muscle activity is sensitive and was effective in detecting changes in tissues from sesbania-treated birds which had not shown any clinical signs. The results also support the possibility that smooth muscle involvement could be a primary cause of toxicity in sesbania poisoning.
mesh_terms: D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D006632:Histamine / Q000494:pharmacology; D007082:Ileum / Q000503:physiopathology; D008168:Lung / Q000503:physiopathology; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects / Q000503:physiopathology*; D010939:Plant Poisoning / Q000209:etiology / Q000503:physiopathology / Q000662:veterinary*; D011201:Poultry Diseases / Q000209:etiology / Q000503:physiopathology*
chemical_list: D006632:Histamine; D002217:Carbachol