High-performance liquid chromatographic comparison of in vivo and in vitro drug metabolism.
Using 2-ethyl-3-(4- hydroxybenzoyl )benzofuran ( EHBB ) as an example, biotransformation in rabbits and rats and by rat hepatocyte suspensions was studied by high-performance liquid chromatography (HPLC) and mass spectrometry. The biotransformation of N-alkyl-substituted piperidines by rat hepatocytes gives valuable information about the pharmacodynamics of this series of compounds. It is demonstrated that a simple reversed-phase HPLC pre-column technique is much superior to the classical sample purification via extraction. The utility of hepatocytes for the investigation of drug metabolism is demonstrated.
D000818:Animals; D001553:Benzbromarone / Q000031:analogs & derivatives / Q000378:metabolism*; D001572:Benzofurans / Q000378:metabolism*; D001646:Bile / Q000378:metabolism; D001711:Biotransformation; D001826:Body Fluids / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D010880:Piperidines / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D013045:Species Specificity
D001572:Benzofurans; D004364:Pharmaceutical Preparations; D010880:Piperidines; C004528:benzarone; D001553:Benzbromarone