The effects of ouabain in the medullary site of the hypotensive action of clonidine.
Blood pressure was studied in pentobarbital anesthetized rats and cats after central administration of ouabain. Intracerebroventricular (i.c.v.) injections caused a classical biphasic effect, a short lasting hypotension followed by a hypertensive phase. When injected directly into the nucleus reticularis lateralis region (NRL), ouabain (0.01-2 micrograms/kg) caused a dose-dependent pressor effect. In the same region, kryptofix 221, a sodium complexing agent, produced a fall in blood pressure. Moreover, central administration of ouabain prevented the hypotensive effect of i.v. clonidine whereas the central hypotensive effect of muscimol was not affected. It is concluded that sodium movements play an important role in the blood pressure regulation within the NRL region. We also report here that ouabain antagonizes the hypotensive effect of clonidine suggesting that sodium movement might be the essential link of this action.
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D001643:Bridged Bicyclo Compounds / Q000494:pharmacology; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002415:Cats; D002614:Chelating Agents / Q000494:pharmacology; D003000:Clonidine / Q000008:administration & dosage / Q000494:pharmacology*; D007022:Hypotension / Q000503:physiopathology; D007276:Injections, Intraventricular; D008526:Medulla Oblongata / Q000502:physiology* / Q000503:physiopathology; D010042:Ouabain / Q000494:pharmacology*; D051381:Rats
D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002614:Chelating Agents; C006071:cryptating agent 222; D010042:Ouabain; D003000:Clonidine