Induction of immunoglobulin-producing human peripheral blood lymphocytes in serum-free medium.
Induction of immunoglobulin-secreting cells from human peripheral blood lymphocytes in a serum-free culture medium was studied. Albumin, transferrin, insulin and fibronectin can replace serum entirely for support of pokeweed mitogen (PWM)-stimulated B lymphocytes, measured by a reverse hemolytic plaque assay using protein A-coated red cells. In this serum-free system, growth and maturation to IgM and IgG secretion occur at the same or higher efficiency as in conventional serum-containing medium, with maximum numbers of plaque-forming cells on day 6 at optimal dose of PWM, 0.5-5 micrograms/ml. This system can be used to avoid the interference from undefined serum components.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D005353:Fibronectins / Q000494:pharmacology; D006462:Hemolytic Plaque Technique; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D007501:Iron / Q000494:pharmacology; D008213:Lymphocyte Activation; D011043:Pokeweed Mitogens / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D014168:Transferrin / Q000494:pharmacology
D003470:Culture Media; D005353:Fibronectins; D007328:Insulin; D011043:Pokeweed Mitogens; D014168:Transferrin; D012710:Serum Albumin, Bovine; D007501:Iron