Different effect of LPS-induced macrophage factor on antibody responses to TI-1 and TI-2 antigens.
Effect of macrophage culture fluid (MF) on thymic independent (TI) antibody responses was examined. MF potentiated antibody responses of spleen cells to dinitrophenyl (DNP)-Ficoll and DNP-liposome, TI-2 antigens, but not to trinitrophenyl (TNP)-BA and TNP-LPS, TI-1 antigens. The enhancing effect of MF on the anti-DNP-Ficoll response was dose-dependent. Neither T cells nor macrophages were required for MF to exert the effect, suggesting that MF works on B cells directly. B cells modulated by MF in their antibody responses were indicated to be in mature B-cell subset for the following reasons: (i) the cells were in (CBA/N x BALB/c) F1 female but not in F1 male mice; (ii) the cells bore the receptors for C3 on their surface. MF was indicated to exert the enhancing effect on the antibody response by modulating the proliferation and/or early events in differentiation of B cells and not by promoting antibody secretion. The active component of the MF was indicated to be Interleukin 1.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D002448:Cell Adhesion; D002478:Cells, Cultured; D004136:Dinitrobenzenes / Q000276:immunology; D005260:Female; D006462:Hemolytic Plaque Technique; D007375:Interleukin-1 / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology
D000941:Antigens; D004136:Dinitrobenzenes; D007375:Interleukin-1; D008070:Lipopolysaccharides; D014301:Trinitrobenzenes