Cell cycle related phenothiazine effects on adriamycin transport.
In P388 murine leukemic cells, the pheno-thiazine tranquilizer, chlorpromazine, causes a marked increase in intracellular drug retention. Laser excited flow cytometry shows that in log phase cultures this increase in drug retention is not uniform but confined to a sub-population. In cell cycle phase enriched populations of P388 cells obtained by centrifugal elutriation, the phenothiazine enhanced adriamycin retention is seen predominantly in cells in the late S, G2/M part of the cell cycle.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects*; D002746:Chlorpromazine / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000032:analysis; D004317:Doxorubicin / Q000378:metabolism*; D005434:Flow Cytometry; D007834:Lasers; D007941:Leukemia P388 / Q000503:physiopathology*; D007942:Leukemia, Experimental / Q000503:physiopathology*; D051379:Mice
D004273:DNA, Neoplasm; D004317:Doxorubicin; D002746:Chlorpromazine