Non-correspondence of [3H]GABA uptake and GAD localization in goldfish amacrine cells.
Ultrastructural analysis of the inner synaptic layer of goldfish retina, using a double-label technique, showed that [3H]GABA uptake and glutamic acid decarboxylase (GAD)-immunoreactivity (IR) occurred in different neuronal processes in most cases. Many [3H]GABA-accumulating processes were found surrounding GAD-IR processes, though not necessarily in a postsynaptic relationship. Co-localization of [3H]GABA uptake and GAD-IR occurred only when one GAD-IR process was juxtaposed to another GAD-IR process. This study suggests that [3H]GABA uptake may be a poor marker for GABA releasing neurons.
D000818:Animals; D001345:Autoradiography; D003530:Cyprinidae / Q000378:metabolism*; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D006054:Goldfish / Q000378:metabolism*; D006651:Histocytochemistry; D007120:Immunochemistry; D008854:Microscopy, Electron; D009474:Neurons / Q000378:metabolism; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase