[Microanalysis of plasma isoniazid and acetylisoniazid by high performance liquid chromatography].
A method for the analysis of isoniazid and acetylisoniazid in plasma or serum has been developed. This report describes a simple, quick and economically optimized reversed-phase high-performance liquid chromatographic assay using a small sample size without solvent extraction step. Isoniazid was condensed with cinnamic aldehyde after trichloroacetic acid deproteinisation. The method correlated well with a fluorimetric procedure. Precision, sensibility and accuracy were good. Common antituberculous drugs did not interfere.
D000171:Acrolein / Q000031:analogs & derivatives; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003720:Densitometry; D006801:Humans; D007538:Isoniazid / Q000031:analogs & derivatives* / Q000097:blood*; D013050:Spectrometry, Fluorescence / Q000379:methods
C006377:(N)1-acetylisoniazid; D000171:Acrolein; C012843:cinnamaldehyde; D007538:Isoniazid
Microdosage de l'isoniazide et de l'acetylisoniazide plasmatiques par chromatographie liquide à haute performance.