[Automatization of heparin determination using chronometric evaluation of anti-Xa activity of the AT III-heparin complex].
The authors describe an adaptation on a semi- automatique coagulometer KC 10 of a manual assay of anti-Xa activity of the Heparin-Antithrombin III Complex close to the technique described by Yin and al. ( Hepaclot - Stago ). The results show a good linearity and a good reproducibility, with a better sensitivity than the manuel method. Freezing has no influence. Significant differences were observed between our assay and the manuel anti-IIa assay. These last results neither depend on the administration route nor on the heparin batches.
D000990:Antithrombin III / Q000032:analysis*; D001322:Autoanalysis / Q000379:methods; D005170:Factor X / Q000037:antagonists & inhibitors*; D006493:Heparin / Q000097:blood*; D006801:Humans; D011516:Prothrombin / Q000037:antagonists & inhibitors
D000990:Antithrombin III; D011516:Prothrombin; D005170:Factor X; D006493:Heparin
Automatisation du dosage de l'héparine par évaluation chronométrique de l'activité anti-Xa du complexe AT III-héparine.