[Etoposide effect on cell cycle kinetics of a human lung cancer cell line].
The lethal and kinetic effects of Etoposide on a cell line of human small cell carcinoma of the lung (PC-6) were analyzed in vitro using flow cytometric measurement of DNA content. The drug effect is dependent on concentration and exposure time. When PC-6 cells were exposed to Etoposide for 1 hour then washed, a increase of cells in G2 phase was observed followed by prompt recovery. Continuous treatment with Etoposide for 24 hours caused a retardation of S phase transit, and high concentrations (greater than 10 micrograms/ml) resulted in stoppage of the whole cell cycle. This drug may be useful in combination chemotherapy regimens.
D002452:Cell Count; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004273:DNA, Neoplasm / Q000032:analysis; D005047:Etoposide / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D011034:Podophyllotoxin / Q000031:analogs & derivatives*
D004273:DNA, Neoplasm; D005047:Etoposide; D011034:Podophyllotoxin