The placental form of glutathione S-transferase as a new marker protein for preneoplasia in rat chemical hepatocarcinogenesis.
A neutral form of glutathione S-transferase (GST-P), which has a subunit of 26,000 (or 21,500) daltons and charge isomers with isoelectric points of 6.7 (major), 6.3 and 6.0, was purified from rat placenta cytosol. Immunohistochemical staining using anti-GST-P rabbit antibody demonstrated that GST-P, which is hardly detectable in normal liver, is markedly increased and localized in preneoplastic hepatic lesions such as hyperplastic nodules, indicating that GST-P could be a useful marker proteins for preneoplasia in chemical hepatocarcinogenesis.
D000818:Animals; D004796:Clinical Enzyme Tests; D003600:Cytosol / Q000201:enzymology; D005260:Female; D005982:Glutathione Transferase / Q000032:analysis / Q000302:isolation & purification*; D006651:Histocytochemistry; D007527:Isoenzymes / Q000032:analysis / Q000302:isolation & purification*; D008099:Liver / Q000201:enzymology; D008113:Liver Neoplasms / Q000139:chemically induced / Q000175:diagnosis*; D010920:Placenta / Q000201:enzymology*; D011230:Precancerous Conditions / Q000139:chemically induced / Q000175:diagnosis*; D011247:Pregnancy; D051381:Rats
D007527:Isoenzymes; D005982:Glutathione Transferase