A simple test for acetylator phenotype using caffeine.
A method is presented for the use of caffeine, in the forms commonly ingested by a large proportion of the world's population, to test for the clinically important acetylation polymorphism. Each of 146 subjects provided a spot sample of urine between 2 and 6 h after coffee, tea or cola soft drink consumption, and the molar ratio of 5-acetylamino-6- formylamino -3-methyluracil ( AFMU ) to 1-methylxanthine (1X) was determined by a simple h.p.l.c. assay. The ratio afforded segregation of three apparent modes of acetylation capacity in this population, in concordance with a standard sulphamethazine phenotyping procedure and with other methods using controlled caffeine intake and urine collections. The day-to-day consistency of the method was established in eight selected subjects.
D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D002110:Caffeine / Q000378:metabolism; D002625:Chemistry, Organic; D002648:Child; D002675:Child, Preschool; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D006801:Humans; D008875:Middle Aged; D055601:Organic Chemistry Phenomena; D010641:Phenotype; D013418:Sulfamethazine / Q000378:metabolism; D014498:Uracil / Q000031:analogs & derivatives / Q000378:metabolism; D014970:Xanthines / Q000378:metabolism
D014970:Xanthines; C008514:methylxanthine; D002110:Caffeine; D013418:Sulfamethazine; D014498:Uracil; C036692:5-acetylamino-6-formylamino-3-methyluracil; D003577:Cytochrome P-450 Enzyme System; D000123:Acetyltransferases