title: Triacylglycerol synthesis in goat mammary gland. Factors influencing the esterification of fatty acids synthesized de novo.
abstract: ATP alone had no effect on incorporation of fatty acids synthesized de novo and membrane-bound diacylglycerol into triacylglycerol. Combined addition of ATP and Mg2+ totally inhibits incorporation of fatty acids synthesized de novo and stimulated incorporation of membrane-bound diacylglycerol. ATP, Mg2+ and glycerol 3-phosphate stimulate incorporation of fatty acids synthesized de novo into triacylglycerol, but inhibited the incorporation of membrane-bound diacylglycerol. Diacylglycerol generated in situ was shown to be superior to diacylglycerols preloaded on the membrane as substrate for the diacylglycerol acyltransferase. A model is proposed to explain the effect of absorbed exogenous fatty acid on fatty acid synthesis de novo in goat mammary gland.
mesh_terms: D000217:Acyltransferases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D051048:Diacylglycerol O-Acyltransferase; D004075:Diglycerides / Q000378:metabolism; D004951:Esterification; D005227:Fatty Acids / Q000096:biosynthesis*; D005260:Female; D005994:Glycerophosphates / Q000494:pharmacology; D006041:Goats; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism*; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D010171:Palmitoyl Coenzyme A / Q000494:pharmacology; D011247:Pregnancy; D014280:Triglycerides / Q000096:biosynthesis*
chemical_list: D004075:Diglycerides; D005227:Fatty Acids; D005994:Glycerophosphates; D014280:Triglycerides; D010171:Palmitoyl Coenzyme A; D000255:Adenosine Triphosphate; C029620:alpha-glycerophosphoric acid; D000217:Acyltransferases; D051048:Diacylglycerol O-Acyltransferase; D008274:Magnesium