[Characterization of slow reacting substance of anaphylaxis in asthmatic sputum].
Sputum from asthmatics contained a mediator similar to slow reacting substance of anaphylaxis (SRS-A), capable of inducing contraction of guinea pig ileum. This mediator possessed the major characteristics of SRS-A, including stability in both neutral and alkaline solutions, lability in boiling acidic solution, destruction by arylsulfatase, inhibition by FPL 55712 and resistance to diphenhy dramine . Thus, we proved that the "SRS-A like" mediator was in fact SRS-A. The activity of histamine in these patients' sputa could be inhibited only by diphenhydramine, but could not be altered by any of the other treatments noted above.
D000328:Adult; D000818:Animals; D001192:Arylsulfatases / Q000494:pharmacology; D001249:Asthma / Q000378:metabolism*; D002867:Chromones / Q000494:pharmacology; D004155:Diphenhydramine / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D006632:Histamine / Q000032:analysis; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D009119:Muscle Contraction / Q000187:drug effects; D013189:SRS-A / Q000032:analysis* / Q000494:pharmacology; D013183:Sputum / Q000032:analysis*
D002867:Chromones; D013189:SRS-A; D006632:Histamine; D004155:Diphenhydramine; C006312:FPL 55712; D001192:Arylsulfatases