The effect of surface sugars on liposomes in immunopotentiation.
The effect of surface sugars of liposomes on the immunological responses to entrapped antigen has been investigated. alpha-Mannose and beta-galactose were grafted on the surface of liposomes containing lysozyme by covalent coupling of p-aminophenyl-D-glycosides to phosphatidyl ethanolamine liposomes using glutaraldehyde. Subcutaneous administration of antigen entrapped in beta-galactose liposomes stimulated an antibody response comparable to that elicited by sugar-free neutral liposomes. However, alpha-mannose bearing liposomes with entrapped lysozyme elicited an immune response similar to that induced by lysozyme in saline. Based on these observations it is suggested that alpha-mannose liposomes, that are specifically recognized by macrophages, are taken up rapidly by receptor mediated endocytosis and that the entrapped antigen is then rapidly degraded, resulting in low antibody production.
D000818:Animals; D000917:Antibody Formation; D002241:Carbohydrates / Q000276:immunology*; D005690:Galactose / Q000276:immunology; D008081:Liposomes / Q000276:immunology*; D008358:Mannose / Q000276:immunology; D009113:Muramidase / Q000276:immunology; D011817:Rabbits
D002241:Carbohydrates; D008081:Liposomes; D009113:Muramidase; D008358:Mannose; D005690:Galactose