title: An SEM analysis of the epithelial--mesenchymal interface in the mandible of the embryonic chick.
abstract: In this paper the ultrastructural features of the epithelial-mesenchymal interface in mandibular processes of embryonic chicks have been examined using scanning electron microscopy. Mandibular epithelium is required for the mesenchyme to differentiate as osteoblasts and to deposit the membrane bones of the mandible. The surface morphology of the epithelium changes from the lateral to the medial face of the mandible from rounded cells, each with a central cilium to flattened cells with numerous microvilli. Treatment with trypsin and pancreatin was used to digest the basal lamina so as to separate epithelium from mesenchyme. This exposed a thick, fibrillar basement membrane (reticular lamina), which was thicker underlying the caudal epithelium than under the cephalad epithelium. Addition of collagenase to the trypsin/pancreatin solution degraded some of the basement lamella, especially that underlying epithelium on the caudal portion of each mandibular process. Selective degradation of basement lamella is postulated as one means of regulating inductive epithelial-mesenchymal interactions. EDTA was used to isolate basal laminae on mandibular mesenchyme. SEM was used to confirm the integrity of the basal lamina, its structure, and its association with overlying epithelial cells and underlying basement lamella.
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002642:Chick Embryo / Q000033:anatomy & histology*; D004848:Epithelium / Q000648:ultrastructure; D008334:Mandible / Q000196:embryology* / Q000648:ultrastructure; D008445:Maxillofacial Development; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000648:ultrastructure; D010012:Osteogenesis