Sequences in the promoter region of the chicken lysozyme gene required for steroid regulation and receptor binding.
We have constructed a series of deletion mutants in the lysozyme promoter region fused to the SV40 T-antigen coding region. Regulated expression was tested after microinjection of the lysozyme deletion mutants into primary cultures of chicken oviduct cells using fluorescent antibodies against T antigen. Deletion of lysozyme gene sequences upstream of position - 164 was accompanied by loss of both progesterone- and glucocorticoid-induced expression. Using the rat liver glucocorticoid receptor for binding studies, two separate binding sites have been identified: a strong binding site that is destroyed by deletion of lysozyme sequences between positions -74 and -39 and a weaker binding site contained between positions -208 and -161 upstream of the lysozyme cap site.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002872:Chromosome Deletion; D005260:Female; D005796:Genes; D006367:HeLa Cells / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009113:Muramidase / Q000235:genetics*; D009154:Mutation; D009876:Operon; D010057:Oviducts / Q000378:metabolism; D010957:Plasmids; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D014162:Transfection
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D009113:Muramidase