title: Nucleotide sequence of initiator tRNA from Mycobacterium smegmatis.
abstract: The nucleotide sequence of initiator tRNA from Mycobacterium smegmatis was determined to be pCGCGGGGUGGAGCAGCUCGGDAGCUCGCUGGGCUCAUAACCCAGAGm7GUCG CAGGU psi CGm1AAUCCUGUCCCCGCUACCAOH . The nucleotide sequence of Mycobacterium initiator tRNA was found to be the same as that of Streptomyces initiator tRNA, except that G46 and A57 were replaced by m7G46 and G57 , respectively. The striking feature of Mycobacterium initiator tRNA is the absence of ribothymidine at residue 54, and the presence of 1-methyladenosine at residue 58 which makes the sequence of this tRNA similar to that of eukaryotic initiator tRNA.
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives / Q000032:analysis; D001483:Base Sequence; D009161:Mycobacterium / Q000235:genetics*; D009690:Nucleic Acid Conformation; D012343:RNA, Transfer / Q000235:genetics* / Q000302:isolation & purification; D013045:Species Specificity; D013302:Streptomyces / Q000235:genetics
chemical_list: C002230:1-methyladenosine; D012343:RNA, Transfer; D000241:Adenosine