Glycosylated proteins as an indicator of metabolic control in diabetes: evaluation by aminophenylboronic acid affinity chromatography.
Glycosylated proteins, glycosylated albumin and HbA1 were studied in type I and type II diabetes. Glycosylated proteins were evaluated by a new method: aminophenylboronic acid affinity chromatography. A good correlation was found between total HbA1 and glycosylated proteins in both groups (r = 0.57, p less than 0.05; r = 0.67, p less than 0.01, respectively), but a positive correlation between stable HbA1 and glycosylated proteins was present only in maturity onset diabetics (r = 0.71, p less than 0.01). Glycosylated proteins correlated with glycosylated albumin only in type II diabetes (r = 0.67, p less than 0.01). We hypothesize that in maturity onset diabetes glycosylated proteins and HbA1 reflect a greater glycemic stability while in insulin-dependent diabetes the same proteins reflect a different periodic pattern of glycemic control. Moreover, our data suggest that aminophenylboronic acid affinity chromatography is a suitable tool for routine monitoring of metabolic control in maturity onset diabetes, while further investigations are needed to establish if this method is a useful index of altered glucose metabolism in type I diabetes.
D001798:Blood Proteins / Q000032:analysis*; D001897:Boronic Acids; D002846:Chromatography, Affinity / Q000379:methods; D003920:Diabetes Mellitus / Q000188:drug therapy; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D006442:Glycated Hemoglobin / Q000032:analysis; D017127:Glycation End Products, Advanced; D006023:Glycoproteins; D006801:Humans; D007004:Hypoglycemic Agents / Q000627:therapeutic use; D012709:Serum Albumin / Q000032:analysis*; D000093422:Glycated Serum Proteins; D000093423:Glycated Serum Albumin
D001798:Blood Proteins; D001897:Boronic Acids; D006442:Glycated Hemoglobin; D017127:Glycation End Products, Advanced; D006023:Glycoproteins; D007004:Hypoglycemic Agents; D012709:Serum Albumin; D000093422:Glycated Serum Proteins; D000093423:Glycated Serum Albumin; C028592:3-aminobenzeneboronic acid