Theoretical aspects of one-point calibration: causes and effects of some potential errors, and their dependence on concentration.
The potential errors of the one-point calibration technique are described mathematically. I examine the theoretical causes and effects of analytical errors in absorbance and of errors in determining calibration concentration, and discuss techniques for minimizing their impact. The dependence of these errors on the calibration concentration and on the size of the result is considered, and some conclusions are drawn about the choice of calibration concentration.
D002138:Calibration; D002624:Chemistry, Clinical / Q000295:instrumentation* / Q000592:standards; D006801:Humans; D008433:Mathematics; D008962:Models, Theoretical; D013053:Spectrophotometry / Q000295:instrumentation* / Q000379:methods