Reciprocal effects of excess potassium salts in the culture medium on type I and type III collagen synthesis by human skin fibroblasts.
By the addition of excess KCl or CH3COOK in the culture medium of human skin fibroblasts, production of non-collagenous proteins and membrane transport of [3H]proline of the cells were activated, but collagen synthesis was not increased. Production of type I collagen was inhibited under these culture conditions but type III collagen synthesis was activated; thus the ratio of type I to type III collagen decreased from 10 to near 1, indicating that the regulation of these two types of collagen is independent in human skin fibroblasts.
D000085:Acetates / Q000494:pharmacology; D019342:Acetic Acid; D000328:Adult; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis* / Q000302:isolation & purification; D003470:Culture Media; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D011188:Potassium / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology; D012867:Skin / Q000187:drug effects / Q000378:metabolism*
D000085:Acetates; D003470:Culture Media; D011189:Potassium Chloride; D003094:Collagen; D019342:Acetic Acid; D011188:Potassium