The separation of collagen alpha-chains by reversed-phase high-performance liquid chromatography. Comparison of column alkyl stationary phases and temperature effects.
Procedures for the separation of alpha 1(I), alpha 2(I), alpha 1(II) and alpha 1 (III) chains of human collagen by reversed-phase high-performance liquid chromatography are described. The influence of several different chromatographic parameters (stationary phase, mobile phase and temperature) has been examined and procedures to optimise resolution presented. These reversed-phase high-performance liquid chromatographic conditions also permit the separation of collagen alpha 1(I), alpha 2(I), alpha 1(II) and alpha 1(III) monomers from their corresponding dimeric beta- and gamma-components.
D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D003094:Collagen / Q000032:analysis / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D013696:Temperature
D000596:Amino Acids; D003094:Collagen