Rapid and quantitative recovery of DNA fragments from gels by displacement electrophoresis (isotachophoresis).
The use of displacement electrophoresis (synonymous to isotachophoresis, steady-state stacking, and moving boundary electrophoresis) for recovery of DNA fragments from agarose and polyacrylamide gels is described. Complete recovery of DNA molecules ranging from oligonucleotides to 20 000-basepairs-long fragments was achieved. The DNA is recovered in a small volume (0.1-0.3 ml) and can be used directly in enzyme-mediated cleavage and ligation reactions. The recovered DNA contained no inhibitory contaminants as revealed by ligation or restriction enzyme cleavage.
D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification*; D004274:DNA, Recombinant; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods; D009838:Oligodeoxyribonucleotides / Q000302:isolation & purification*; D009841:Oligonucleotides / Q000302:isolation & purification*; D010957:Plasmids
D004274:DNA, Recombinant; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D004247:DNA