Precolumn concentration of protein samples by displacement electrophoresis (isotachophoresis) followed by high-performance molecular sieve chromatography on a packed agarose column.
The use of displacement electrophoresis for the concentration of dilute protein solutions and the construction of a column suitable for this purpose are described. The concentrated protein zone can be pumped directly from the electrophoresis column into a gel-filtration column, which greatly reduces losses of protein. Recoveries of 95% or better were obtained even for small amounts of protein. The electrophoretically concentrated samples gave virtually the same elution profiles as did samples injected in a small volume without the use of electrophoretic preconcentration.
D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D014168:Transferrin / Q000032:analysis
D011506:Proteins; D014168:Transferrin