Formation of 19-nor-10-keto-25-hydroxyvitamin D3 in cultured mammalian cells.
Cultured dog kidney cells convert 25-hydroxyvitamin D3 into more polar metabolites during in vitro incubations. One elutes with 1,25-dihydroxyvitamin D3 from high pressure liquid chromatographic silica columns with hexane:isopropanol (9:1), but can be separated from 1,25-dihydroxyvitamin D3 by elution with dichloromethane:isopropanol (95:5). This peak has been isolated, purified and identified by mass spectral analysis to be 19-nor-10-keto-25-hydroxyvitamin D3.
D000818:Animals; D002112:Calcifediol / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism; D002460:Cell Line; D002855:Chromatography, Thin Layer; D004285:Dogs; D007668:Kidney; D013058:Mass Spectrometry
C039030:19-nor-10-keto-25-hydroxyvitamin D3; D002112:Calcifediol