Acyl-acyl carrier protein as substrate of the acyltransferase of rat liver microsomes.
Acyl-acyl carrier protein (acyl-ACP) can serve as well as acyl-CoA as substrate of the 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) acyltransferase of rat-liver microsomes. The product of the acylation with either thioester substrate is predominantly phosphatidylcholine (PC) (92-95%). The acyl-group transferred from either myristoyl-CoA or myristoyl-ACP is located at the C-2 position of the phospholipid (PL). The apparent Km values for the myristoyl-CoA and myristoyl-ACP were 46 microM and 63 microM, and the corresponding apparent Vmax values were 1.0 and 1.6 nmol/min/mg. The rate of acylation with the acyl-ACP was unaffected by the addition of free CoA-SH. These data suggest that acyl-CoA and acyl-ACP are transferred to 1-acyl-GPC by the same or similar enzyme systems.
D000213:Acyl Carrier Protein / Q000378:metabolism*; D000214:Acyl Coenzyme A / Q000378:metabolism*; D000215:Acylation; D000818:Animals; D007700:Kinetics; D008862:Microsomes, Liver / Q000201:enzymology*; D010713:Phosphatidylcholines / Q000378:metabolism; D051381:Rats
D000213:Acyl Carrier Protein; D000214:Acyl Coenzyme A; D010713:Phosphatidylcholines; C040349:S-tetradecanoyl-coenzyme A