title: Effects of in vivo estradiol administration on availability of rat uterine nuclear acceptor in measured in vitro.
abstract: Estradiol injected in vivo successfully completes for binding to uterine nuclear acceptor sites measured by the assay of Kon and Spelsberg (1). Such competition is time-and dose-dependent, with maximal inhibition (75%) 1 h after injection and at a dose of 10 micrograms of 17 beta-estradiol per 300 g of BW. Thus, this assay appears to accurately measure those uterine nuclear acceptor sites mediating estrogen action in vivo.
mesh_terms: D000818:Animals; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D008722:Methods; D051381:Rats; D011919:Rats, Inbred Strains; D011960:Receptors, Estrogen / Q000378:metabolism*; D013997:Time Factors; D014599:Uterus / Q000378:metabolism*
chemical_list: D011960:Receptors, Estrogen; D004958:Estradiol