A transport-dependent energy burden imposed by growth of Enterobacter cloacae in the presence of 10% sodium dodecyl sulfate.
Growth of Enterobacter cloacae in a glucose asparagine salts medium in the presence of 10% sodium dodecyl sulfate entailed an energy burden in the form of a 20% decreased cell yield, a 30% faster rate of glucose utilization, and a 70% increased rate of oxygen consumption. Similar detergent-induced decreases in cell yield were observed with 10 other sugars and sugar alcohols. Only glycerol supported equivalent cell growth in the presence and absence of sodium dodecyl sulfate. A model is presented which interprets these observations in terms of an altered membrane potential which makes active transport energetically less efficient.
D001693:Biological Transport, Active / Q000187:drug effects; D003902:Detergents / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004734:Energy Metabolism / Q000187:drug effects*; D004754:Enterobacter / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D004755:Enterobacteriaceae / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*
D003902:Detergents; D012967:Sodium Dodecyl Sulfate; D005947:Glucose