Schistosoma mansoni: interactive effects of irradiation and cryopreservation on parasite maturation and immunization of mice.
Mechanically transformed schistosomula of Schistosoma mansoni were irradiated with levels of 60Co irradiation between 2.5 and 54 krad, cryopreserved by the two-step addition of ethanediol and rapid cooling technique, and were injected intramuscularly into groups of mice which were perfused 40 days later. The schistosomula were either irradiated and then cryopreserved (IC) or cryopreserved and then irradiated in the frozen state (CI). Development into adult worms was prevented with 4 krad for IC schistosomula, but for CI schistosomula a small number of worms (1.6%) was recovered using 8.8 krad. A dose of 4 krad was sufficient to prevent development of unfrozen controls (I), but for schistosomula irradiated while exposed to ethanediol (EI), a dose of 7 krad was required. Using the different protocols, the peak levels of protection against a challenge infection were achieved with 9 (IC) and 16 krad (CI), compared to 20 krad for unfrozen schistosomula (I) reported previously. The highest level of protection (65%) was achieved with CI schistosomula. Possible interactions between the radioprotective and damaging effects of cryopreservation are discussed.
D000818:Animals; D003451:Cryoprotective Agents / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D005615:Freezing; D007114:Immunization; D008297:Male; D051379:Mice; D011309:Preservation, Biological; D011837:Radiation-Protective Agents / Q000494:pharmacology; D012550:Schistosoma mansoni / Q000254:growth & development / Q000276:immunology / Q000528:radiation effects*; D012552:Schistosomiasis / Q000276:immunology*
D003451:Cryoprotective Agents; D011837:Radiation-Protective Agents