Visualization of pronuclei in living bovine zygotes.
Bovine embryos were surgically collected from the oviducts of superovulated crossbred heifers 48 h postonset of estrus. The one-celled ova were treated with 4'-6'-diamidino-2-phenylindole (DAPI) and observed under ultraviolet light by fluorescence microscopy. Both male and female pronuclei were visualized, identified and subjected to micromanipulation.
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000648:ultrastructure*; D005260:Female; D005306:Fertilization; D008856:Microscopy, Fluorescence; D011247:Pregnancy; D013480:Superovulation; D015053:Zygote / Q000648:ultrastructure*