Effect of staphylococcal alpha-hemolysin upon anion transport in the rabbit erythrocyte.
Equilibrium exchange of SO4(2-) was measured prior to and during hemolysis in rabbit erythrocytes exposed to staphylococcal alpha-hemolysin. The anion-transport protein of the rabbit erythrocyte has also been identified. Equilibrium exchange of SO4(2-) was measured by both efflux and influx of 35SO4(2-). The rate of influx of SO4(2-) in rabbit erythrocytes exposed to alpha-hemolysin was twice that of the untreated cells. The rate of SO4(2-) efflux was unchanged by alpha-hemolysin. Inhibition of anion exchange with 4,4'-diisothiocyano-2,2'-stilbenedisulfonic acid (DIDS) did not inhibit hemolysis, therefore, the increased influx of SO4(2-) may occur through a DIDS-insensitive pathway.
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000031:analogs & derivatives*; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism*; D001693:Biological Transport, Active / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000494:pharmacology*; D006358:Hot Temperature; D011817:Rabbits; D013431:Sulfates / Q000097:blood*
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D013431:Sulfates; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; C018564:dihydro-DIDS; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid