title: 1-Acetyl-3-acetoxy-5'5-diphenylhydantoin has colchicine-like activity.
abstract: 1-Acetyl-3-acetoxy-5'5-diphenylhydantoin (Ac-DPH) is a synthetic derivative of diphenylhydantoin with unusual properties: 1) it inhibits microtubular polymerization and depolymerizes established microtubules; 2) it blocks colchicine binding and displaces colchicine from microtubular protein, although it bears no resemblance to colchicine; and 3) it does not arrest human lymphocytes in metaphase, in contrast to diphenylhydantoin or its metabolite, 5-(4-hydroxyphenyl)-5-phenylhydantoin (HPPH).
mesh_terms: D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D002850:Chromatography, Gel; D003078:Colchicine / Q000378:metabolism* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000166:cytology / Q000648:ultrastructure; D008677:Metaphase / Q000187:drug effects*; D008854:Microscopy, Electron; D008870:Microtubules / Q000187:drug effects*; D008940:Mitotic Index; D010672:Phenytoin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D013552:Swine; D014404:Tubulin / Q000378:metabolism*
chemical_list: D014404:Tubulin; C041562:1-acetyl-3-acetoxy-5',5-diphenylhydantoin; D010672:Phenytoin; D003078:Colchicine