[Interaction of 8-aza-16-oxasteroids with rat liver microsomal cytochrome P-450].
The interaction of the 8-aza-16-oxasteroid series (8-AS) with cytochrome P-450 from liver microsomes of intact and phenobarbital-induced rats has been studied. It has been shown that 8-AS are the substrates for the cytochrome P-450-dependent enzyme system, and that their affinity for cytochrome P-450 is determined by the structure of the compounds tested. Using inhibitory analysis, the site in the active center of hemoprotein responsible for 8-AS binding was examined. The possibility of direct participation of the 8-AS ketogroup in their binding with cytochrome P-450 is discussed.
D000818:Animals; D001378:Azasteroids / Q000378:metabolism*; D001665:Binding Sites; D002784:Cholesterol / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008797:Metyrapone / Q000494:pharmacology; D008862:Microsomes, Liver / Q000201:enzymology*; D010634:Phenobarbital / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry; D013260:Steroids, Heterocyclic / Q000378:metabolism*; D013329:Structure-Activity Relationship
D001378:Azasteroids; D013260:Steroids, Heterocyclic; D003577:Cytochrome P-450 Enzyme System; D002784:Cholesterol; D011433:Propranolol; D010634:Phenobarbital; D008797:Metyrapone
Vzaimodeĭstvie 8-aza-16-oksasteroidov s tsitokhromom P-450 mikrosom pecheni krys.