Automated fibronectin assay using light-scattering optics on a centrifugal analyzer.
We describe an automated, kinetic nephelometric method for fibronectin on the Multistat III F/LS Centrifugal Analyzer (Instrumentation Laboratory Inc., Lexington, MA 02173). Antiserum is diluted with polyethylene glycol. Calibrators and samples are prediluted with potassium phosphate buffer (10 mmol/L, pH 7.0) containing 8.5 g of NaCl per litre. Intensity (I) is read at 5 and 180 s and the resulting delta I plotted against concentration. A non-linear least squares curve fitting and interpolation of results is carried out automatically. Three controls, with values of between 190-370 gave coefficients of variation between 4 and 7 percent, and the sensitivity of the method is to 25 mg/L.
D000328:Adult; D001322:Autoanalysis / Q000379:methods; D002498:Centrifugation / Q000379:methods; D003424:Crohn Disease / Q000097:blood; D005353:Fibronectins / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D007231:Infant, Newborn; D008027:Light; D008297:Male; D009391:Nephelometry and Turbidimetry / Q000379:methods; D012016:Reference Values; D012542:Scattering, Radiation; D013997:Time Factors
D005353:Fibronectins; D007202:Indicators and Reagents