Induction of polyploid nuclei in Physarum polycephalum by cycloheximide treatment in prophase.
Macroplasmodia of the acellular slime mold Physarum polycephalum were treated with pulses of cycloheximide (10 micrograms/ml medium, for 3 h), initiated 10-20 min before metaphase in the synchronous nuclear division cycle. This treatment interfered with normal division of the nuclei, but permitted DNA synthesis in the next S phase. This interpretation is supported by measurements of the DNA content per nucleus in cycloheximide-treated cultures as compared to control cultures, which show that some nuclei after cycloheximide treatment are polyploid. By this method we can produce polyploid strains of Physarum, but the elevated nuclear DNA content is not stable, and after several months the strains have reverted to the normal diploid DNA content.
D002453:Cell Cycle; D002467:Cell Nucleus / Q000187:drug effects*; D003520:Cyclophosphamide / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D010804:Physarum; D011418:Prophase
D003520:Cyclophosphamide; D004247:DNA