High-performance liquid chromatographic determination of tocopherols and tocotrienols and its application to diets and plasma of Finnish men. I. Analytical method.
A HPLC Method is described for the determination of tocopherols and tocotrienols in human diets and plasma. After a room-temperature saponification diet samples were extracted with n-hexane. A direct hexane extraction was used for plasma samples. Using a normal-phase column at elevated temperature and a fluorescence detector complete separation of all four tocopherols, alpha-, beta-, gamma-tocotrienols and BHA and good reproducibility and sensitivity were obtained. The recovery of tocopherols added to diet samples was 99% for alpha-tocopherol, 95% for beta-tocopherol, 99% for gamma-tocopherol and 80% for delta-tocopherol. The recovery of alpha-tocopherol added into plasma was 99%.
D002083:Butylated Hydroxyanisole / Q000032:analysis; D002839:Chromans; D002851:Chromatography, High Pressure Liquid; D005526:Food, Formulated / Q000032:analysis*; D006586:Hexanes; D006801:Humans; D006878:Hydroxides; D008297:Male; D011188:Potassium; D017680:Potassium Compounds; D012997:Solvents; D024508:Tocotrienols; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood
D002839:Chromans; D006586:Hexanes; D006878:Hydroxides; D017680:Potassium Compounds; D012997:Solvents; D024508:Tocotrienols; D014810:Vitamin E; D002083:Butylated Hydroxyanisole; C026385:n-hexane; C013649:plastochromanol 8; C082089:tocotrienol, beta; C082032:tocotrienol, alpha; D011188:Potassium; C029943:potassium hydroxide