[Comparative effects of 4 cephalosporins on the incorporation of tritiated diaminopimelic acid during bacterial growth].
When comparing antibiotic activities, it might be of interest to study parameters other than minimal inhibitory concentrations (MIC's). Specific activity of beta-lactams on bacterial cell wall makes it possible to determine radiolabelled diaminopimelic acid (DAP) incorporation in growing cultures. We have studied the effects of various concentrations of cefalotin , cefotaxime, latamoxef (moxalactam) and ceftiolene (42 980 RP) on DAP incorporation in 6 strains of E. coli, E. cloacae and S. aureus. Drug concentration which inhibits 90 % of radioactivity incorporation (CII 90) was found to vary from 0.1 X MIC to 3 X MIC. This fact suggests that beta-lactam action on cell wall synthesis and/or structure and MIC's are not always strictly correlated.
D000599:Amino Acids, Diamino / Q000378:metabolism*; D002511:Cephalosporins / Q000494:pharmacology*; D003960:Diaminopimelic Acid / Q000378:metabolism*; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D014316:Tritium
D000599:Amino Acids, Diamino; D002511:Cephalosporins; D014316:Tritium; D003960:Diaminopimelic Acid
Effects comparés de quatre céphalosporines sur l'incorporation d'acide diaminopimélique tritié au cours de la croissance bactérienne.