125I labelled inulin: a convenient marker for deposition of liposomal contents in vivo.
The utility of an iodinated derivative of inulin (125I-tyraminyl-inulin, 125ITI) for reporting in vivo tissue distributions of liposomal contents is described. It is shown, employing a rat model, that this probe satisfies the criteria that the free form is rapidly cleared from the circulation and excreted, whereas 125ITI encapsulated in large unilamellar vesicle (LUV) systems and subsequently taken up in various tissues exhibits a long (greater than 3 days) retention time. Further, high specific activities (greater than 1 muCi per microliter) are easily achievable, allowing low LUV dose levels (less than or equal to 2.5 mumole phospholipid/kg body weight) to be employed. Minimal tissue workups for quantitation of 125ITI distributions are required. It is concluded that from criteria of sensitivity, expense and simplicity, 125ITI is a most convenient probe for characterizing liposome deposition in vivo.
D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D005260:Female; D007444:Inulin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D008081:Liposomes / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D007457:Iodine Radioisotopes; D008081:Liposomes; D010713:Phosphatidylcholines; C041998:iodo-tyraminyl inulin; D007444:Inulin; D002784:Cholesterol