Improved procedure for extracting aminoglycosides from renal cortical tissue.
An efficient and reproducible procedure was developed for extracting aminoglycosides from renal cortical tissue. It involves a double homogenization and two rinsings with trichloroacetic acid. A higher recovery is obtained compared with that of other previously reported methods.
D000583:Amikacin / Q000302:isolation & purification; D000617:Aminoglycosides / Q000302:isolation & purification; D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D005260:Female; D005839:Gentamicins / Q000302:isolation & purification; D007672:Kidney Cortex / Q000032:analysis*; D009428:Netilmicin / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D005839:Gentamicins; D009428:Netilmicin; D000583:Amikacin