Subcellular distribution of acetylcholinesterase asymmetric forms during postnatal development of mammalian skeletal muscle.
This study describes the changes which occur in intra- and extracellular asymmetric acetylcholinesterase (AChE) forms in rat gracilis muscle during postnatal development. Initially (day 7) these forms (12.5 S and 16 S AChE) were evenly distributed along the muscle and only present intracellularly. With advancing age (days 7-28) they gradually became concentrated in endplate (vs non-endplate) muscle regions where a certain proportion of them was subsequently externalized. In contrast, no externalization was observed in the non-endplate regions. Our results support the view that AChE asymmetric forms are assembled within the muscle cell prior to their deposition on the extracellular synaptic compartment.
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D007527:Isoenzymes / Q000378:metabolism*; D009045:Motor Endplate / Q000201:enzymology; D024510:Muscle Development; D009132:Muscles / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000201:enzymology; D014018:Tissue Distribution
D007527:Isoenzymes; D000110:Acetylcholinesterase