The use of fresh-frozen tissue in diagnostic transmission electron microscopy.
We have examined the effects of freezing on fresh pathologic human tissue specimens with regard to the fine structural preservation of various organelles that can be of diagnostic importance. Specimens included in this study were frozen either in the cryostat (-20 degrees C) or by quenching in isopentane cooled by liquid nitrogen (-115 degrees C). The results indicate that fresh-frozen tissue that is subsequently fixed and processed for transmission electron microscopy (TEM) provides ultrastructural information that is very nearly equal to that of conventionally processed specimens. Additionally, we performed identical freezing experiments using animal (rat) tissue in order to ensure that the results we obtained using a rather limited number of human specimens were valid. The outcome of these animal experiments thoroughly supports our observations using human pathologic tissue. We believe an appreciation of the fact that fresh-frozen tissue is suitable for TEM examination may become a very valuable resource in the surgical pathology laboratory.
D000818:Animals; D005615:Freezing; D006651:Histocytochemistry; D006801:Humans; D008722:Methods; D008854:Microscopy, Electron; D009584:Nitrogen; D010420:Pentanes; D051381:Rats
D010420:Pentanes; D009584:Nitrogen