Comparison of immediate and delayed inoculation of HEp-2 cells for isolation of respiratory syncytial virus.
Immediate inoculation of HEp-2 cells is generally advocated for the isolation of respiratory syncytial virus. However, delayed laboratory inoculation of properly transported specimens obtained by aspiration of nasopharyngeal mucus provided an isolation rate similar to that obtained with immediate inoculation.
D002478:Cells, Cultured; D006801:Humans; D009305:Nasopharynx / Q000382:microbiology*; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*; D014776:Virus Cultivation / Q000379:methods