Inhibition of endoribonuclease VI from Artemia larvae by cytidine 2'-phosphate.
The endoribonuclease VI from Artemia larvae is non-competitively inhibited by cytidine 2'-phosphate with a Ki ca 1 microM. Neither of the cytidine monophosphates isomers with the phosphate group in the 3' or 5' position nor the cyclic 2':3' phosphate are inhibitors at concentrations up to 100 microM. Adenosine, guanosine and uridine 2' or 3' phosphates are also ineffective in this range of concentrations. Certain polyribonucleotides are potent competitive inhibitors of the ribonuclease activity.
D001156:Artemia / Q000201:enzymology*; D003568:Cytidine Monophosphate / Q000494:pharmacology*; D003597:Cytosine Nucleotides / Q000494:pharmacology*; D004722:Endoribonucleases / Q000037:antagonists & inhibitors*; D006868:Hydrolysis; D007814:Larva / Q000201:enzymology; D011072:Poly U / Q000378:metabolism; D011131:Polyribonucleotides / Q000494:pharmacology; D013379:Substrate Specificity
D003597:Cytosine Nucleotides; D011131:Polyribonucleotides; D011072:Poly U; D004722:Endoribonucleases; C036214:endoribonuclease VI, Artemia; D003568:Cytidine Monophosphate