title: Enzymatic catalysis in organic media at 100 degrees C.
abstract: Porcine pancreatic lipase catalyzes the transesterification reaction between tributyrin and various primary and secondary alcohols in a 99 percent organic medium. Upon further dehydration, the enzyme becomes extremely thermostable. Not only can the dry lipase withstand heating at 100 degrees C for many hours, but it exhibits a high catalytic activity at that temperature. Reduction in water content also alters the substrate specificity of the lipase: in contrast to its wet counterpart, the dry enzyme does not react with bulky tertiary alcohols.
mesh_terms: D000438:Alcohols / Q000378:metabolism; D000818:Animals; D002384:Catalysis; D004798:Enzymes / Q000378:metabolism*; D006358:Hot Temperature; D008049:Lipase / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology; D012996:Solutions; D013552:Swine; D014280:Triglycerides / Q000378:metabolism; D014867:Water / Q000378:metabolism
chemical_list: D000438:Alcohols; D004798:Enzymes; D012996:Solutions; D014280:Triglycerides; D014867:Water; D008049:Lipase; C005830:tributyrin