A separation and purification procedure for [14C] di-2-ethylhexyl phthalate in fish.
A procedure utilizing the reverse isotope dilution analysis is presented for the determination of di-2-ethylhexyl phthalate (DEHP) and total metabolites in tissues of fish and water samples. This procedure offers several advantages: it eliminates laborious and expensive use of column chromatography and identification by gas chromatography/mass spectrometry analysis; it does not require quantitative separation; it selectively removes fish lipids by partitioning across a hexane/acetonitrile system; it uses an internal standard, di-pentylphthalate, for the gas-liquid chromatographic analysis; and it overcomes interference from background DEHP.
D000818:Animals; D002250:Carbon Radioisotopes; D004051:Diethylhexyl Phthalate / Q000302:isolation & purification*; D005399:Fishes / Q000378:metabolism*; D010795:Phthalic Acids / Q000302:isolation & purification*; D014873:Water Pollutants / Q000302:isolation & purification*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
D002250:Carbon Radioisotopes; D010795:Phthalic Acids; D014873:Water Pollutants; D014874:Water Pollutants, Chemical; D004051:Diethylhexyl Phthalate