Properties of ferredoxin reductase and ferredoxin from the bovine corpus luteum.
Ferredoxin reductase and ferredoxin were purified from the bovine corpus luteum and their properties compared to the corresponding adrenal proteins. The luteal and adrenal proteins had similar absorbance spectra and molecular weights. Evidence was obtained from spectrophotometric titrations for formation of 1:1 complexes between luteal ferredoxin reductase and ferredoxin and between ferredoxin and cytochrome P-450scc. Adrenal ferredoxin reductase and ferredoxin were equally as effective as luteal ferredoxin reductase and ferredoxin in supporting cholesterol side-chain cleavage by luteal cytochrome P-450scc.
D000311:Adrenal Glands / Q000032:analysis; D000818:Animals; D002417:Cattle; D002784:Cholesterol / Q000378:metabolism; D003338:Corpus Luteum / Q000032:analysis* / Q000201:enzymology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005287:Ferredoxin-NADP Reductase / Q000302:isolation & purification*; D005288:Ferredoxins / Q000302:isolation & purification*; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000302:isolation & purification*; D009249:NADP / Q000378:metabolism
D005288:Ferredoxins; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D002784:Cholesterol; D005287:Ferredoxin-NADP Reductase; D009247:NADH, NADPH Oxidoreductases