[Effect of castration on purine biosynthesis in the rat liver and kidney].
With the present study, the effects of testosterone deficiency on the biosynthesis of purine nucleotides and nucleic acids in rat liver and kidney has been investigated. 20% homogenates were prepared in 1 N HCl04, centrifuged and the nucleic acid were extracted from the precipitate with 0.7 N PCA (15 min at 90 degrees C): the acid-soluble purines were precipitated with 1 M AgNO3 from the supernatant of the centrifuged homogenate, then extracted with 1 M HC1. An increase of the specific activity of the liver (A, +49%; G, +47%) and of the kidney (A, +54%; G, +34%) has been observed (P less than 0.05), while no variation of the specific activity of nucleic acids was evident after castration.
D000225:Adenine / Q000378:metabolism; D000818:Animals; D002369:Castration; D005561:Formates / Q000378:metabolism; D006147:Guanine / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D011685:Purine Nucleotides / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains
D005561:Formates; D011685:Purine Nucleotides; C030544:formic acid; D006147:Guanine; D000225:Adenine
Effetto delĺa castrazione sulla biosintesi purinica nel fegato e nel rene di ratto.