Development of a stable reference material for prostatic acid phosphatase.
We describe the development of a stable reference material for prostatic acid phosphatase, derived from human prostatic tissue and human seminal fluid. The enzyme was purified by an L-tartramic acid affinity-chromatography technique. Two-dimensional electrophoresis revealed essentially no contaminating proteins, and specific tests revealed no contaminating enzymes. The preparations, in a matrix containing 30 g of human serum albumin and 0.1 mol of sodium acetate per liter, pH 6.0, were studied with respect to stability of both catalytic activity and immunological identity. We conclude that the preparations from either source are satisfactorily stable, and that either is acceptable for use in preparing clinical reference materials. These materials will be used in developing a reference method.
D000135:Acid Phosphatase / Q000302:isolation & purification / Q000592:standards*; D000704:Analysis of Variance; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D008297:Male; D011467:Prostate / Q000201:enzymology*; D012015:Reference Standards; D012661:Semen / Q000201:enzymology; D013696:Temperature
D000135:Acid Phosphatase