Quantitative analysis of wide-field endothelial specular photomicrographs.
Samples from the center of a wide-field specular micrograph adequately assessed cell density and frequency distribution of cell areas. A preliminary analysis of the spread of values indicated whether an increased sample size was required. An algorithm determined how large a sample taken from the central photograph (three, five, or nine rectangles) was necessary to permit detection of a 10% change in cell area. This analysis of sample size also indicated that sampling by small-field specular microscopy was not representative of the surrounding square millimeter of corneal endothelium.
D002452:Cell Count; D003315:Cornea / Q000166:cytology*; D004727:Endothelium / Q000166:cytology; D006801:Humans; D010784:Photomicrography / Q000379:methods*