Application of polyethyleneimine cellulose for the class separation of steroidal carboxylic acids from neutral steroids and pigments in urine.
Metabolites of corticosteroids that contain the 21-oic acid moiety are found in human urine. The acids from neutral steroids and urinary pigments have been separated by passing the mixture through a column of polyethyleneimine cellulose. The acids adhering to the column are quantitatively eluted with dilute formic acid. The purified preparation is suitable for derivatization and chromatographic analysis.
D000305:Adrenal Cortex Hormones / Q000652:urine*; D000328:Adult; D002264:Carboxylic Acids / Q000302:isolation & purification / Q000652:urine*; D002482:Cellulose / Q000031:analogs & derivatives*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer / Q000379:methods; D003900:Desoxycorticosterone / Q000652:urine; D006801:Humans; D007231:Infant, Newborn; D010860:Pigments, Biological / Q000652:urine*; D011094:Polyethyleneimine / Q000031:analogs & derivatives; D011095:Polyethylenes; D011283:Pregnenes / Q000652:urine; D012588:Scintillation Counting
D000305:Adrenal Cortex Hormones; D002264:Carboxylic Acids; D010860:Pigments, Biological; D011095:Polyethylenes; D011283:Pregnenes; C018367:polyethyleneimine-cellulose; C035466:20-hydroxy-3-oxo-4-pregnen-21-carboxylic acid; D003900:Desoxycorticosterone; D011094:Polyethyleneimine; D002482:Cellulose