title: Amino acid composition of Clostridium botulinum type B neurotoxin.
abstract: To characterize type B botulinum neurotoxin based on reliable data on the amino acid composition, three batches of the neurotoxin were analyzed. Each batch was isolated from a separate neurotoxin producing bacterial culture (strain Okra). Two batches were purified by the same method and one was purified by a different method. The toxin preparations were comparable in purity (judged by polyacrylamide gel--sodium dodecyl sulfate electrophoresis) and similar in amino acid composition. The best estimate of the number of amino acid residues per toxin molecule (mol. wt 152,000) was: Asp212,Thr54,Ser83,Glu130,Pro46,Gly61++ +,Ala44,Val54,CyS11,Met23,Ile144,Leu107 , Tyr81,Phe77,Lys118,His7,Arg39,Trp18.
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D001905:Botulinum Toxins; D055598:Chemical Phenomena; D002621:Chemistry; D006868:Hydrolysis; D009498:Neurotoxins
chemical_list: D000596:Amino Acids; D009498:Neurotoxins; D001905:Botulinum Toxins