title: Isolation and characterization of a genetic variant of bovine proinsulin.
abstract: A genetic variant of bovine proinsulin has been isolated using preparative reverse-phase HPLC. The new proinsulin (bovine proinsulin II) differs from the known proinsulin (bovine proinsulin I) by a single amino acid residue at position C-48 in the connecting peptide. The amino acid replacement is a leucine substitution for proline. The two proinsulins were found in a ratio of approximately 9:1, proinsulin I: proinsulin II. No chemical or biological differences were observed for the two proinsulins other than their different elution times on reverse-phase HPLC.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002096:C-Peptide / Q000235:genetics; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D014644:Genetic Variation; D006801:Humans; D011384:Proinsulin / Q000235:genetics / Q000302:isolation & purification*; D013045:Species Specificity; D013552:Swine
chemical_list: D002096:C-Peptide; D011384:Proinsulin