Morphological and proliferative characteristics of human breast tumor cells cultured on plastic and in collagen matrix.
Collagen, a major component of the extracellular matrix, is important in maintaining the in vivo characteristics of epidermal cells in vitro. In the present study, the morphological and proliferative characteristics of two human mammary epithelial cell lines (T-47D and MCF-7) cultured in cowhide collagen (Vitrogen 100) were studied. When grown in collagen, the tumor cells displayed a spherical shape and formed multilayered, tumorlike aggregates; desmosomes were observed between cells. In contrast, both cell lines grew as monolayers on plastic substratum; cells were characteristically flat and polygonal. When grown in collagen matrix, the human breast cancer cells became more dependent on serum for growth: cells proliferated in the presence of 10% fetal bovine serum (FBS) but failed to grow in 1% serum. On the other hand, these cells proliferated rapidly in 1% serum when they were grown on plastic. Even in 10% serum the doubling time of cells cultured in collagen was longer than that of cells maintained on plastic. In addition, cells cultured in collagen proliferated rapidly in a serum-free medium containing insulin, epidermal growth factor (EGF), estrogen, and transferrin. The collagen gel system may be useful for characterizing physiologically important trophic factors that regulate the proliferation and other functions of human breast tumor cells.
D001943:Breast Neoplasms / Q000473:pathology*; D002455:Cell Division; D002460:Cell Line; D003094:Collagen; D003584:Cytological Techniques; D005260:Female; D006801:Humans; D007700:Kinetics; D008854:Microscopy, Electron; D010969:Plastics
D010969:Plastics; D003094:Collagen