Benzyloxycarbonylphenylalanylcitrulline p-nitroanilide as a substrate for papain and other plant cysteine proteinases.
After preliminary assays, with papain, bromelain and ficin, on a range of citrulline p-nitroanilides, values of Km and kcat. for the papain-catalysed hydrolysis of three derivatives, N alpha- benzyloxycarbonylcitrulline p-nitroanilide, benzyloxycarbonylphenylalanylcitrulline p-nitroanilide and benzyloxycarbonylglycylphenylalanylcitrulline p-nitroanilide, were obtained. It is concluded that benzyloxycarbonylphenylalanylcitrulline p-nitroanilide is a highly selective substrate for the sensitive detection and assay of the plant cysteine proteinases.
D001963:Bromelains / Q000378:metabolism; D002956:Citrulline / Q000031:analogs & derivatives / Q000378:metabolism; D004151:Dipeptides / Q000378:metabolism; D005361:Ficain / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D009842:Oligopeptides / Q000378:metabolism; D010206:Papain / Q000378:metabolism*; D010944:Plants / Q000201:enzymology*
D004151:Dipeptides; D009842:Oligopeptides; D002956:Citrulline; C041186:N-benzyloxycarbonylcitrulline 4-nitroanilide; C041187:benzyloxycarbonylglycyl-phenylalanyl-citrulline 4-nitroanilide; C041185:N-benzyloxycarbonylphenylalanyl-citrulline 4-nitroanilide; D001963:Bromelains; D010206:Papain; D005361:Ficain