Preferential reaction of the carcinogen N-acetoxy-2-acetylaminofluorene with satellite DNA.
The carcinogens N-acetoxy-2-acetylaminofluorene (N-acetoxy-AAF) and N-hydroxy-2-aminofluorene (N-hydroxy-AF) were incubated with calf thymus DNA to determine if reaction occurred preferentially with discrete regions within the DNA. Derivative melting profiles indicated that both compounds decreased satellite transitions and that N-acetoxy-AAF depressed the melting of higher temperature regions. These data suggest that N-acetoxy-AAF reacted to a greater extent with G + C-rich regions and, because the resulting adduct disrupted the helix, the cooperativity of melting decreased. Reaction of N-acetoxy-AAF with purified satellite III DNA confirmed the preferential interaction of this carcinogen with G + C-rich regions as compared to main component DNA. The derivative melting profile of lambda DNA in the presence of actinomycin D further demonstrated that this type of analysis can detect preferential interactions with specific DNA sequences.
D015073:2-Acetylaminofluorene / Q000031:analogs & derivatives*; D000099:Acetoxyacetylaminofluorene / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D001482:Base Composition; D002273:Carcinogens / Q000378:metabolism; D002417:Cattle; D004276:DNA, Satellite / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D005449:Fluorenes / Q000378:metabolism / Q000494:pharmacology; D006358:Hot Temperature; D009691:Nucleic Acid Denaturation / Q000187:drug effects
D002273:Carcinogens; D004276:DNA, Satellite; D005449:Fluorenes; D003609:Dactinomycin; D000099:Acetoxyacetylaminofluorene; C001368:N-hydroxy-2-aminofluorene; D015073:2-Acetylaminofluorene