title: Electrophoretic patterns of protein synthesis and turnover in apical plasma membrane and outer bilayer of Schistosoma mansoni.
abstract: Polypeptide fractions labelled with [14C]leucine and associated with fractionated inner plasma membrane and outer bilayer (envelope) from the apical double bilayer complex of the surface epithelium of the human blood fluke, Schistosoma mansoni, were analyzed by two-dimensional electrophoresis and fluorography. In contrast to the distribution of alkaline phosphatase, the polypeptide profiles of the two bilayer fractions were similar due to cross contamination between one membrane containing larger amounts of protein (inner) and the second bilayer having more heavily labelled proteins (outer bilayer). Convincing evidence for only two of 35 polypeptides could be provided for localization to the outer bilayer. These results suggest that the marker enzyme used for the inner bilayer, alkaline phosphatase, may not be homogeneously distributed in this membrane. In pulse-chase studies a correction factor for cross-contamination was derived. The rate of turnover of the polypeptide fractions was twice as fast for the outer compared to the inner membrane, this difference being consistent with the view that multilamellar bodies are the precursors of the apical double bilayer complex. Comparing the rates of surface renewal in adult and juvenile schistosomes leads to the suggestion that membrane turnover can be correlated with susceptibility to host immune effector mechanisms.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000032:analysis / Q000378:metabolism*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000378:metabolism; D005260:Female; D006207:Half-Life; D007930:Leucine / Q000378:metabolism; D008051:Lipid Bilayers / Q000032:analysis / Q000378:metabolism*; D008297:Male; D010455:Peptides / Q000302:isolation & purification; D014176:Protein Biosynthesis; D012550:Schistosoma mansoni / Q000378:metabolism*
chemical_list: D008051:Lipid Bilayers; D010455:Peptides; D007930:Leucine