The influence on the dermal cellular infiltrate of topical steroid applications and vehicles in guinea pig skin: normal skin, allergic and toxic reactions.
The mechanisms of the anti-inflammatory effects of corticosteroids are uncertain but could be explained by an influence on infiltrating leukocytes. Our method for the qualitative and quantitative investigation of the dermal cellular infiltrate makes it possible to study the effects of topically applied corticosteroid preparations and vehicles on the infiltrating leukocytes of normal skin, allergic and toxic reactions in guinea pig skin. Ointment and cream vehicles as well as corticosteroid cream and ointment preparations often caused erythema and increased mononuclear infiltrate after only short periods of application (24-72 h). The strongest steroid ointment gave the most marked macroscopic response and propylene glycol preparations the most marked cellular response. In both toxic and allergic reactions, application of steroid preparations after the provocation gave no beneficial result either macroscopically or microscopically. Macroscopic scores were worsened by cream and ointment preparations. Although steroid solutions had no beneficial effect, they caused no detrimental effect. The guinea pig seems to be extremely sensitive to irritants and has not proved to be a suitable model for this approach to the study of the efficacy of topically applied steroids.
D000287:Administration, Topical; D000305:Adrenal Cortex Hormones / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D003436:Croton Oil / Q000494:pharmacology / Q000633:toxicity; D003877:Dermatitis, Contact / Q000276:immunology* / Q000473:pathology; D004137:Dinitrochlorobenzene / Q000494:pharmacology / Q000633:toxicity; D006168:Guinea Pigs; D009824:Ointments; D010081:Oxazolone / Q000494:pharmacology / Q000633:toxicity; D010592:Pharmaceutic Aids / Q000494:pharmacology* / Q000633:toxicity; D012867:Skin / Q000166:cytology / Q000187:drug effects*; D013997:Time Factors
D000305:Adrenal Cortex Hormones; D004137:Dinitrochlorobenzene; D009824:Ointments; D010592:Pharmaceutic Aids; D010081:Oxazolone; D003436:Croton Oil