Determination of phagocytosis of 32P-labeled Staphylococcus aureus by bovine polymorphonuclear leukocytes.
A procedure for the measurement of phagocytosis by bovine polymorphonuclear leukocytes (PMN) of 32P-labeled Staphylococcus aureus was modified so that a larger number of samples could be compared in a single run, and smaller volumes of sample, PMN, and 32P-labeled S aureus could be used. Results were highly reproducible, with a coefficient of variation between duplicate determinations of less than or equal to 2%. Lysostaphin was prepared from the supernatant of S staphylolyticus and was compared with a commercially available preparation. Effects of lysostaphin on PMN and influence of incubation media on release of 32P from 32P-labeled S aureus by lysostaphin were examined.
D000818:Animals; D001433:Bacteriolysis / Q000187:drug effects; D002417:Cattle / Q000276:immunology*; D003126:Colostrum / Q000276:immunology; D003470:Culture Media; D005260:Female; D008248:Lysostaphin / Q000494:pharmacology; D008722:Methods; D008892:Milk / Q000166:cytology / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects; D010761:Phosphorus Radioisotopes; D013211:Staphylococcus aureus / Q000187:drug effects / Q000276:immunology*
D003470:Culture Media; D010761:Phosphorus Radioisotopes; D008248:Lysostaphin