Cell membrane polarity in primary human fibroblasts.
The topologies of bindings of cationized CF and native ferritin (NF) on plasma membranes of primary human fibroblasts were examined by transmission electronmicroscopy. Both ligands bound mainly to apical surfaces and less abundantly to lateral regions. At basal localization they were observed only at cell-to-cell connections. The polarity of negatively charged binding sites was not altered by glutaraldehyde fixation. The definite polarity of plasma membranes of cultured cells express alternating surface areas charged negatively or positively.
D001665:Binding Sites; D002412:Cations; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D004622:Embryo, Mammalian; D005293:Ferritins / Q000378:metabolism*; D005347:Fibroblasts / Q000648:ultrastructure*; D006801:Humans; D007365:Intercellular Junctions; D008854:Microscopy, Electron
D002412:Cations; D005293:Ferritins