Analyte and matrix problems in the calibration and quality assessment of the bromocresol green method for albumin in serum.
Different batches of bromocresol green were used in a study of several modifications of the bromocresol green method for serum albumin. No major batch variation was observed. Lyophilized animal sera and a certain batch of lyophilized human sera, however, responded differently from a pool of fresh human sera when the method was modified. These phenomena should be taken into account in calibration procedures and in quality assessment.
D000818:Animals; D001800:Blood Specimen Collection; D001961:Bromcresol Green / Q000592:standards*; D003408:Cresols / Q000592:standards*; D005069:Evaluation Studies as Topic; D005612:Freeze Drying; D006736:Horses; D006801:Humans; D007202:Indicators and Reagents / Q000592:standards; D011786:Quality Control; D012709:Serum Albumin / Q000032:analysis*
D003408:Cresols; D007202:Indicators and Reagents; D012709:Serum Albumin; D001961:Bromcresol Green