title: Effect of radiation and freezing on [3H]DNA of Yersinia enterocolitica.
abstract: Freezing of the enteropathogenic bacterium Yersinia enterocolitica to -18 and -75 degrees C caused 7 and 42% cell death, respectively, and 0.329 and 0.588 single-strand breaks per 10(8) daltons of DNA, respectively, while radiation to one D10 dose (10% cell survival) combined with freezing to 2 to 0, -18, and -75 degrees C induced 0.05, 0.75, and 5.04 single-strand breaks, respectively. The increase in the effectiveness of radiation with respect to the yield of single-strand breaks at -18 and -75 degrees C is contrary to expectation and seems to be due to arrest of repair of single-strand breaks by these low temperatures.
mesh_terms: D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D004269:DNA, Bacterial / Q000528:radiation effects; D004277:DNA, Single-Stranded / Q000528:radiation effects; D005514:Food Irradiation; D005615:Freezing; D005720:Gamma Rays; D012965:Sodium Chloride / Q000494:pharmacology; D015008:Yersinia enterocolitica / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects
chemical_list: D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D012965:Sodium Chloride