DNA replication in a diploid strain of Saccharomyces cerevisiae homozygous for the rad6-1 mutation.
The generation time of a diploid strain homozygous for the rad6-1 mutation was 160 min, and the duration of the S phase was 80 min; in the parental heterozygote, these values were 90 and 40 min, respectively. Analysis of DNA sedimentation in an alkaline sucrose gradient revealed that heterozygote high-molecular-weight DNA appeared after 60 min, and homozygote high-molecular weight DNA only after a 100-min pulse.
D004271:DNA, Fungal / Q000096:biosynthesis*; D005796:Genes; D007399:Interphase; D007700:Kinetics; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014466:Ultraviolet Rays
D004271:DNA, Fungal