Adenylate cyclase activity and localization in necrotic and non-necrotic areas of rat myocardium after injection of high doses of isoproterenol.
With the help of electron microscopical cytochemical investigation, adenylate cyclase activity was shown to be strongly reduced in the necrotic tissue 24 h after isoproterenol injection. However, no changes were found in the basal activity of adenylate cyclase in this tissue region by biochemical determination, while the isoproterenol-stimulated activity was markedly reduced under corresponding conditions in the same tissue. It was suggested that these discrepancies between cytochemical and biochemical findings are due to partial solubilization of the adenylate cyclase protein by damaging the membrane structure.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D009202:Cardiomyopathies / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D006651:Histocytochemistry; D007545:Isoproterenol / Q000633:toxicity*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009336:Necrosis; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D000262:Adenylyl Cyclases; D007545:Isoproterenol