Comparison of two superfusion systems for study of neurotransmitter release from rat cerebral cortex slices.
Depolarization-induced release of [3H]gamma-aminobutyric acid (GABA) and [3H]noradrenaline (NA) from rat cerebral cortex slices was studied in two superfusion systems: one with stationary and the other one with continuously shaken slice compartments. Calcium-dependent depolarization-induced release of GABA and NA could be demonstrated only with shaken slices. GABA, but not NA, could also be released by high K+ media and veratridine from stationary slices. Synaptic transmitter releasing mechanisms are apparently damaged in stationary slices, possibly due to impaired energy metabolism.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D004734:Energy Metabolism; D008297:Male; D008722:Methods; D009638:Norepinephrine / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D000249:Adenosine Monophosphate; D005680:gamma-Aminobutyric Acid; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D002118:Calcium; D009638:Norepinephrine