Intense furosemide-sensitive potassium accumulation in astrocytes in the presence of pathologically high extracellular potassium levels.
An intense K+ accumulation in primary cultures of astrocytes, occurring when external K+ was increased from 5.4 to 54 mM, was investigated. This increase resulted in a doubling of the K+ content within 10 s. Thirty percent of the accumulation was inhibited by furosemide (2 mM). This drug had no effect on the unidirectional influx of K+ at 5.4 mM K+, but when the extracellular K+ concentration was increased, there appeared to be a furosemide-sensitive component of the influx. This component increased with increasing external K+ levels, reaching 44% of the total influx at 72 mM. These results show that astrocytes exhibit an intense furosemide-sensitive K+ accumulation which is activated by K+ levels resembling those occurring in the extracellular compartment during pathological events. Previous studies on a furosemide-sensitive Cl- pump in cultured astrocytes suggest that this accumulation might be via KCl cotransport, which in other systems is involved in volume control.
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001693:Biological Transport, Active / Q000187:drug effects; D005110:Extracellular Space / Q000378:metabolism; D005665:Furosemide / Q000494:pharmacology*; D006706:Homeostasis / Q000187:drug effects; D009115:Muridae; D011188:Potassium / Q000378:metabolism*
D005665:Furosemide; D011188:Potassium