Determination of imidazole acetic acid and its conjugate(s) levels in urine, serum and tissues of rats: studies on changes in their levels under various conditions.
A convenient and reproducible method for assay of imidazole acetic acid (ImAA) was developed as a modification of that described previously (Watanabe et al., 1983). ImAA conjugate(s) (ImAA-C), mainly consisting of imidazole acetic acid riboside, could be measured by this method after its hydrolysis to ImAA. The ImAA and ImAA-C levels in various tissues of rats were measured and the effects of various agents on these levels were studied. The renal clearance values of ImAA-C in rats and man were similar to the creatinine clearance values, but those of ImAA were 1/40 of those of ImAA-C, suggesting that the latter is readily excreted in the urine. Consistent with this idea, the urinary excretion of ImAA-C was found to increase much more than that of other histamine metabolites during late pregnancy, when the foetus produces much histamine.
D000818:Animals; D002927:Cimetidine / Q000494:pharmacology; D003404:Creatinine / Q000652:urine; D005260:Female; D005333:Fetus / Q000378:metabolism; D006146:Guanidines / Q000494:pharmacology; D007093:Imidazoles / Q000097:blood / Q000378:metabolism* / Q000652:urine; D008297:Male; D008657:Metabolic Clearance Rate; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D006146:Guanidines; D007093:Imidazoles; D002927:Cimetidine; D003404:Creatinine; C005954:imidazoleacetic acid; C004479:pimagedine