title: The inhibition of both initiation and elongation of adenovirus DNA replication by actinomycin D.
abstract: The effect of actinomycin D on adenovirus DNA replication has been examined both in vivo and in a cell-free extract capable of replication on exogenously added template. In both cases we show that 5 micrograms/ml of drug cause an inhibition of DNA synthesis of at least 80%. The in vitro results further demonstrate that both DNA chain growth (elongation) and initiation - the addition of the first nucleotide of the DNA chain (dCMP) to the preterminal protein - are inhibited directly by the drug, by not by alpha-amanitin.
mesh_terms: D000256:Adenoviridae / Q000187:drug effects* / Q000254:growth & development; D004261:DNA Replication / Q000187:drug effects*; D004279:DNA, Viral / Q000096:biosynthesis*; D003609:Dactinomycin / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D003609:Dactinomycin