Induction of laurate omega-hydroxylase by di (2-ethylhexyl)phthalate in rat liver microsomes.
Liver microsomes, prepared from rats fed a diet containing di (2-ethylhexyl)phthalate, were observed to hydroxylate lauric acid at carbon 12 at a specific activity 6 times greater than control rats. There was no increase in the specific activity of the laurate 11-hydroxylase. The specific activity of hepatic microsomal NADPH-cytochrome c reductase was increased 2-fold by phthalate feeding, but no effect was observed in the specific contents of cytochromes b5 or P-450. These results indicate that di (2-ethylhexyl)phthalate is similar to the hypolipidemic agent and peroxisomal proliferator , clofibrate, which was previously reported to be a novel inducer of the cytochrome P-450 mediated omega-hydroxylation of fatty acids.
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D042926:Cytochrome P-450 CYP4A; D003580:Cytochromes / Q000378:metabolism; D004051:Diethylhexyl Phthalate / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*; D009929:Organ Size / Q000187:drug effects; D010795:Phthalic Acids / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D003580:Cytochromes; D010795:Phthalic Acids; D004051:Diethylhexyl Phthalate; D006899:Mixed Function Oxygenases; D042926:Cytochrome P-450 CYP4A