Further evidence for the DNA base specificity of light-induced banding.
Experiments have been carried out to investigate the DNA base specificity of light-induced banding (LIB) produced by photo-oxidation of chromosomes followed by Acridine Orange staining to detect denatured DNA. Nuclei of different base composition, human and onion, and fluorochromes of different base specificities and modes of binding to DNA were used. Our results indicate that specific destruction of guanine residues is the main effect of photo-oxidation under the conditions used, and that LIB is a base-specific phenomenon. In addition, photo-oxidation may also cause DNA-protein cross-linking which affects the binding of some dyes, while prolonged photo-oxidation appears to cause more general damage to DNA.
D001482:Base Composition / Q000528:radiation effects; D002467:Cell Nucleus / Q000032:analysis / Q000528:radiation effects; D002871:Chromosome Banding; D002875:Chromosomes / Q000528:radiation effects*; D004247:DNA / Q000032:analysis / Q000378:metabolism / Q000528:radiation effects*; D005456:Fluorescent Dyes; D006801:Humans; D066298:In Vitro Techniques; D008027:Light; D008214:Lymphocytes / Q000032:analysis / Q000528:radiation effects; D009691:Nucleic Acid Denaturation / Q000528:radiation effects*; D010084:Oxidation-Reduction; D010777:Photochemistry; D010944:Plants / Q000032:analysis / Q000528:radiation effects
D005456:Fluorescent Dyes; D004247:DNA