Immunochemical characteristics of Streptococcus mutans serotype h carbohydrate antigen.
Serotype h carbohydrate antigen was prepared from cell walls of Streptococcus mutans strain MFe28 of monkey origin. The h antigen was extracted from the cell walls with 5% trichloracetic acid at 4 C, and purified by DEAE-Sephadex A-25 ion exchange chromatography followed by Sephacryl S-300 gel filtration. The purified antigen was composed of galactose (75%), glucose (16%), and rhamnose (3%). Although the antiserum against whole cells of S. mutans MFe28 gave a strong cross reaction with serotype d S. mutans, serotype h-specific antiserum could be obtained by adequate adsorption. The precipitin reactions and hapten inhibition test using serotype h-specific antiserum showed that galactose, glucose, and their derivative sugars were markedly potent inhibitors. It was concluded that the serotype h antigen is immunologically distinguishable from the known serotypes of S. mutans, although it is closely related to serotype d antigen of S. mutans.
D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002241:Carbohydrates / Q000276:immunology*; D007120:Immunochemistry; D011135:Polysaccharides, Bacterial / Q000276:immunology; D012703:Serotyping; D013295:Streptococcus mutans / Q000145:classification / Q000276:immunology*
D000942:Antigens, Bacterial; D002241:Carbohydrates; D011135:Polysaccharides, Bacterial