Adenovirus VAI RNA facilitates the initiation of translation in virus-infected cells.
The adenovirus VAI RNA is a small polymerase III-transcribed species that is required for optimal translation of mRNAs late after infection. Mutant dl331 fails to produce this RNA species and, as a result, grows poorly. Mutant-infected cells contain normal levels of late mRNAs, but reduced levels of polypeptides are synthesized late after infection. Translational elongation occurs at normal rates in mutant, as compared to wild-type, virus-infected cells. Initiation of translation occurs with reduced efficiency in dl331 -infected cells. VAI RNA is required for formation of a stable 48S preinitiation complex and very likely functions to facilitate the interaction between 43S preinitiation complex and mRNA to form the 48S species.
D000260:Adenoviruses, Human / Q000235:genetics*; D002460:Cell Line; D002472:Cell Transformation, Viral; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000196:embryology; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D010442:Peptide Chain Initiation, Translational; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012320:RNA Polymerase III / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D012270:Ribosomes / Q000378:metabolism
D012333:RNA, Messenger; D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases; D012320:RNA Polymerase III