Characterization of a protein synthesis system from rat liver. Translation of endogenous and exogenous messenger RNA.
An in vitro system prepared from rat liver postmitochondrial supernatant exhibits a high rate of protein synthesis for an extended period of time. This system initiates translation of either endogenous or exogenous mRNA, incorporates Met at a rate of 13 pmol/mg of postmitochondrial supernatant protein/min, maintains this rate for at least 90 min, and performs several rounds of translation/mRNA molecule. Up to 50% of the activity is due to reinitiation of protein synthesis using endogenous mRNA. In addition, 60-70% of the protein synthesized was released from ribosomes into the medium. Addition of globin mRNA stimulates protein synthesis and results in the synthesis of a protein that comigrates with authentic rabbit globin. Black beetle virus mRNA 2 also stimulates protein synthesis and results in synthesis of a protein with molecular weight corresponding to that of the mature viral protein. With endogenous rat liver mRNA this system synthesizes a large number of proteins.
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005470:Fluorometry; D008099:Liver / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D011188:Potassium / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D014176:Protein Biosynthesis; D012314:RNA Cap Analogs / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013095:Spermidine / Q000494:pharmacology; D013997:Time Factors
D000596:Amino Acids; D011480:Protease Inhibitors; D012314:RNA Cap Analogs; D012333:RNA, Messenger; C017516:7-methylguanosine triphosphate; D008274:Magnesium; D011188:Potassium; D013095:Spermidine