Glycosphingolipids of porcine pancreas.
Neutral and acidic glycosphingolipids were purified from porcine pancreas by chromatography on columns of DEAE-Sephadex and Iatrobeads. The chemical structures of the purified glycolipids were determined by carbohydrate analysis, methylation analysis, enzyme treatment, fatty acid analysis, NMR and IR. The major glycolipid of porcine pancreas was Gal(alpha,1-4)Gal(beta,1-)ceramide. Gangliosides GM3 and GD3 were major acidic components and galactosylceramide 3-sulfate was also found.
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005227:Fatty Acids / Q000032:analysis; D006026:Glycoside Hydrolases; D006028:Glycosphingolipids / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D010179:Pancreas / Q000032:analysis*; D013055:Spectrophotometry, Infrared; D013552:Swine
D002241:Carbohydrates; D005227:Fatty Acids; D006028:Glycosphingolipids; D006026:Glycoside Hydrolases