title: Nonthermal effects of ND:YAG laser on biological functions of human skin fibroblasts in culture.
abstract: Previous studies have indicated that laser can selectively affect the biological functions of cells. In the present study, the role of a thermal component in laser-induced alterations in the biology of human skin fibroblasts was examined. Cells were cultured on 96-well tissue culture plates, subjected to treatment with the Nd:YAG laser (wavelength 1,064 nm), and the temperature of the medium was monitored by a microprobe connected to a telethermometer . For comparison, parallel cultures were heated to the same temperatures by tungsten-halogen lamp. The cell cultures were analyzed for collagen synthesis by incubating the cultures with [3H]proline, and the collagen production was assayed by the synthesis of nondialyzable [3H]hydroxyproline. The rate of DNA replication was also determined by measuring the uptake of [3H]thymidine. A marked decrease of collagen production and thymidine incorporation was noted in the cultures subjected to Nd:YAG laser. No such decreases were noted in cultures heated to the corresponding temperatures by tungsten-halogen lamp. The results thus indicate that the biochemical alteration caused by the Nd:YAG laser in human fibroblast functions cannot be explained on the basis of thermal effects.
mesh_terms: D003094:Collagen / Q000096:biosynthesis; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology*; D006219:Halogens; D006358:Hot Temperature; D006801:Humans; D006909:Hydroxyproline / Q000096:biosynthesis; D007834:Lasers; D009354:Neodymium; D012867:Skin / Q000166:cytology*; D014414:Tungsten
chemical_list: D006219:Halogens; D009354:Neodymium; D003094:Collagen; D004247:DNA; D006909:Hydroxyproline; D014414:Tungsten