Schistosoma mansoni: DNA synthesis in males and females from mixed and single-sex infections.
The pattern of DNA synthesis in male and female Schistosoma mansoni from mixed and single-sex infections in mice has been determined biochemically and autoradiographically using the incorporation of [3H]-thymidine as a specific marker of DNA synthesis. During early development (up to 35 days post-infection (p.i.] adult males and females from mixed infections had a comparable specific activity of DNA. In males, from 35 days p.i. onwards, the specific activity of DNA decreased rapidly to become significantly different from that observed in females. In single-sex infections DNA synthesis in females was depressed compared to that observed in males from single-sex infections and was also significantly different (P less than or equal to 0.01) from DNA synthesis in females from mixed infections. There was no significant difference in DNA synthesis between males from single or mixed infections. DNA synthesis in both sexes was found to be sensitive to hydroxyurea treatment, the inhibition in sexually mature females being approximately 75% and in males approximately 45%. Autoradiography using both light microscope and electron microscope techniques revealed that the testes and ovary had labelled cells. In male schistosomes the only other labelled cells were small undifferentiated cells found scattered through the tissues. In females the vitelline gland had large numbers of densely labelled nuclei within Stage 1 vitelline cells, as well as numerous small undifferentiated cells.
D000818:Animals; D001345:Autoradiography; D004247:DNA / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D005260:Female; D006918:Hydroxyurea / Q000494:pharmacology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012550:Schistosoma mansoni / Q000378:metabolism*; D012552:Schistosomiasis / Q000378:metabolism / Q000469:parasitology*; D012727:Sex Characteristics; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D004247:DNA; D013936:Thymidine; D006918:Hydroxyurea