A twin titration microcalorimeter for the study of biochemical reactions.
A small-volume (200 microliter) titration calorimeter of high sensitivity (1 mu cal ) has been developed for the purpose of studying biochemical reactions where the amounts of material are limited to a few nanomoles. High sensitivity is achieved by calorimetric twining , use of glass cells, elimination of vapor space, effective low-energy stirring, and reduction of measurement time. The calorimeter operates using the heat conduction principal with computer-controlled electrical compensation, which reduces the measurement time of each point from 10 to 3 min. This reduction in time is accompanied by a corresponding increase in the precision of measurement. The use of the calorimeter is demonstrated by a measurement of the heat of oxygenation of hemocyanin.
D001671:Biochemistry / Q000295:instrumentation*; D002151:Calorimetry / Q000295:instrumentation* / Q000379:methods; D003201:Computers; D006433:Hemocyanins; D008433:Mathematics; D008832:Microchemistry / Q000295:instrumentation; D010084:Oxidation-Reduction
D006433:Hemocyanins