Typing of Enterobacter spp. by bacteriocin susceptibility and its use in epidemiological analysis.
Most clinical isolates of Enterobacter cloacae are bacteriocinogenic and susceptible to bacteriocins. Both rapidly diffusing, nonsedimentable, protease-susceptible and slowly diffusing, sedimentable, protease-resistant bacteriocins are produced. A practicable system was devised for epidemiological typing of E. cloacae isolates by their patterns of susceptibility to bacteriocins. A set of eight bacteriocin-producing strains was grown on tryptic soy agar plates for 16 h. After removal of the producer lawn, the isolates to be typed were inoculated on the agar media by a multipoint inoculator. After a second 16-h period of incubation, the strains were classified into bacteriocin types according to the patterns of growth inhibition. Typability of 134 clinical isolates was 96.3%. Only 11 (8.2%) of the isolates fell into the largest group. Repeat testing of isolates from the same patients within 2 months gave identical bacteriocin types. Other species of Enterobacter (E. agglomerans and E. aerogenes) are also typable by this method.
D001430:Bacteriocins / Q000494:pharmacology*; D004754:Enterobacter / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification; D004755:Enterobacteriaceae / Q000145:classification*; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests
D001430:Bacteriocins