title: Ultrastructural damage in cochleas used for studies of basilar membrane mechanics.
abstract: Cat cochleas used for interferometric studies of basilar membrane mechanics were examined with the electron microscope. The structures most severely damaged in the experimental cochleas are the outer hair cells and the radial afferent fibers to the inner hair cells. Since the basilar membrane and other supporting structures appear to be normal, mechanical changes observed in the experimental cochleas are most probably due to outer hair cell damage. Individual animals with varying degrees of damage showed large differences in the frequency of basilar membrane resonance at the same place in the cochlea. Shifts in tuning of this magnitude could occur as a consequence of hair cell damage only if the stiffness of the stereocilia and associated structures was greater initially than the stiffness of the basilar membrane and gradually decreased with damage. The present series of observations, therefore, suggest that the stiffness of the outer hair cell stereocilia determines basilar membrane tuning.
mesh_terms: D000818:Animals; D001489:Basilar Membrane / Q000502:physiology*; D002415:Cats; D003051:Cochlea / Q000293:injuries / Q000648:ultrastructure*; D007758:Ear, Inner / Q000502:physiology*; D006198:Hair Cells, Auditory / Q000648:ultrastructure; D006317:Hearing Loss, Noise-Induced / Q000473:pathology; D008854:Microscopy, Electron; D009925:Organ of Corti / Q000648:ultrastructure; D012106:Research; D013680:Tectorial Membrane / Q000648:ultrastructure