Primary sequence differences between Chido and Rodgers variants of tryptic C4d of the human complement system.
Human tryptic C4d of the Chido and Rodgers variant was fragmented by cyanogen bromide and trypsin. The fragments were characterized by amino acid analysis and sequence determination. Polymorphism between the two genetic variants was detected in 5 positions. Four were closely located (residues 141, 142, 145, 146), where Leu, Ser, Ile, His occurred in the Chido variant and Pro, Cys, Leu, Asp in the Rodgers variant, respectively. In position 94 Gly was found in Chido and Asp in Rodgers. Alignment of the fragments was performed and it is concluded that tryptic C4d of both variants contains 346 residues.
D000595:Amino Acid Sequence; D001789:Blood Group Antigens / Q000235:genetics*; D003181:Complement C4 / Q000235:genetics*; D015935:Complement C4b; D003488:Cyanogen Bromide; D014644:Genetic Variation; D006801:Humans; D010446:Peptide Fragments / Q000235:genetics*; D011110:Polymorphism, Genetic; D014357:Trypsin
D001789:Blood Group Antigens; D003181:Complement C4; D010446:Peptide Fragments; D015935:Complement C4b; C032261:complement C4d; D014357:Trypsin; D003488:Cyanogen Bromide