Comparison of in-vitro production of progestagens by the corpora lutea of early pregnancy of the rat and hamster.
Immature rats and adult hamsters were killed on Days 2, 4 or 8 of pregnancy (Day 1 = sperm positive vaginal smear). Dispersed luteal cells (5 X 10(4) cells) were incubated for 2 h in the absence or presence of graded doses of ovine LH. In the absence of LH, incubation of rat luteal cells compared to hamster cells produced about 3-6-fold as much progesterone, 26-66 times as much 20 alpha-dihydroprogesterone and about the same amounts of 17 alpha-hydroxyprogesterone. For the rat, 1 ng LH was the minimal dose which stimulated synthesis of progesterone and 17 alpha-hydroxyprogesterone by luteal cells on Days 2 and 4 whereas 10 ng LH stimulated maximal production of progesterone by Day-8 luteal cells. As pregnancy progressed from Day 2 to Day 8, there was an inverse relationship between the levels of progesterone and 20 alpha-dihydroprogesterone accumulated by rat luteal cells. For the hamster, 1 ng LH significantly stimulated accumulation of progesterone and 17 alpha-hydroxyprogesterone by Day-2 luteal cells but not by Day-4 or Day-8 cells. Hamster luteal cells on Day 4 produced the highest levels of progesterone in response to 10 or 100 ng LH, with a maximal rate of accumulation by Day-8 cells with 10 ng LH.
D019326:17-alpha-Hydroxyprogesterone; D004092:20-alpha-Dihydroprogesterone / Q000378:metabolism; D000818:Animals; D003338:Corpus Luteum / Q000187:drug effects / Q000378:metabolism*; D006224:Cricetinae; D005260:Female; D006908:Hydroxyprogesterones / Q000378:metabolism; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000494:pharmacology; D008647:Mesocricetus; D011247:Pregnancy; D011270:Pregnancy, Animal; D011374:Progesterone / Q000097:blood / Q000378:metabolism*; D051381:Rats
D006908:Hydroxyprogesterones; D004092:20-alpha-Dihydroprogesterone; D011374:Progesterone; D019326:17-alpha-Hydroxyprogesterone; D007986:Luteinizing Hormone