[Biosynthesis of purine nucleotides in the rat muscle after castration].
The paper describes the effects of castration on formate-14C incorporation into the acid-soluble purines and into the nucleic acids of skeletal muscles (ELD and G) and perineal muscle (LAM). 20% homogenates were prepared in 1 M HC10(4), centrifuged and the nucleic acid were extracted from the precipitate with 0.7 N PCA (15 min at 90 degrees C). The acid-soluble purines were precipitated with 1 M AgNO3 from the supernatant of the centrifuged homogenate, then extracted with 1 M HC1 and separated by ion exchange chromatography with a scalar gradient of HC1. In the LAM a significant decrease in the specific activity of the purine bases (A, -56%, P less than 0.01; G, -47%, P less than 0.05) and of the nucleic acid (-47%, P less than 0.01) was observed after castration, while no difference was observed in skeletal muscles.
D000225:Adenine / Q000378:metabolism; D000818:Animals; D002369:Castration; D005561:Formates / Q000378:metabolism; D006147:Guanine / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000378:metabolism*; D011685:Purine Nucleotides / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains
D005561:Formates; D011685:Purine Nucleotides; C030544:formic acid; D006147:Guanine; D000225:Adenine
Biosintesi dei nucleotidi purinici nei muscoli di ratto dopo castrazione.