title: Picrotoxinin and phorbol-12-myristate-13-acetate stimulate the secretion of multiple forms of somatostatin from cultured rat brain cells.
abstract: The molecular forms of somatostatin (SRIF) secreted by cultured fetal rat brain cells were resolved using reverse phase high performance liquid chromatography followed by radioimmunoassay. Multiple forms of SRIF-like immunoactivity were detected in media from cells treated with either picrotoxinin, phorbol-12-myristate-13-acetate, or high potassium. For stimulated cells, elevated levels of an SRIF-28-like molecule, an SRIF-14-like molecule, and a hydrophobic SRIF-like molecule were observed compared to basal conditions. All three forms of SRIF-like molecules were also detected in acid extracts of whole cells. The data are consistent with the possibility that secretion of multiple SRIFs , including SRIF-28, may be regulated by multiple effectors and mechanisms.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D010704:Phorbols / Q000494:pharmacology*; D010852:Picrotoxin / Q000031:analogs & derivatives* / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D054830:Sesterterpenes; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D010455:Peptides; D010704:Phorbols; D010852:Picrotoxin; D011188:Potassium; D054830:Sesterterpenes; D013755:Tetradecanoylphorbol Acetate; C029993:somatostatin-like peptides; C022961:picrotoxinin