title: An automated immunoradiometric assay for human thyrotropin.
abstract: In this two-site immunoradiometric assay for thyrotropin, developed for use in the "Kemtek 3000" automated radioimmunoassay system, commercially available monoclonal antibody to thyrotropin is labeled with 125I, and the solid-phase antibody is an IgG fraction of sheep antiserum to thyrotropin, covalently coupled to reprecipitated aminocellulose. There are two incubations, totalling 3 h, the sensitivity is 0.03 milli-int. unit/L. The mean thyrotropin value for 82 healthy euthyroid subjects was 1.7 milli-int. units/L (range 0.4-3.6). For 19 overtly clinically and biochemically hyperthyroid subjects the values ranged from undetectable to 0.2 milli-int. unit/L. In this assay, euthyroid and hyperthyroid subjects can be distinguished with assay of a single basal sample. The assay appears suitable for routine use as a first-line test of thyroid function.
mesh_terms: D000911:Antibodies, Monoclonal; D001322:Autoanalysis; D006801:Humans; D006980:Hyperthyroidism / Q000097:blood; D008163:Luminescent Measurements; D011863:Radioimmunoassay / Q000379:methods; D012016:Reference Values; D013972:Thyrotropin / Q000097:blood* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D013972:Thyrotropin