[Delayed hypersensitivity reaction to alloantigens in mice].
A new scheme of induction of delayed hypersensitivity (DH) to alloantigens in mice is described. The optimal conditions for DH development are intraperitoneal injection of 1 X 10(7) cells of the allogeneic spleen to normal mice (not treated with cyclophosphamide) 5 days before the challenge with 5 X 10(6) spleen cells. s.c. The maximal response was seen 24 h after resolution. The reaction was specific, being mediated by T lymphocytes; it could not be transferred to intact recipients by allogeneic serum. However, DH was found to be transferrable by s.c. injection of 5 X 10(6) lymphocytes from sensitized donors. Such a modification of the scheme of DH induction may appear useful for studies of the animals' immunity, particularly for studies of the tolerance to allografts.
D000818:Animals; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA
D007519:Isoantigens
Reaktsiia giperchuvstvitel'nosti zamedlennogo tipa k alloantigenam u mysheĭ.