Chromatographic separation of choline, trimethylamine, trimethylamine oxide, and betaine from tissues of marine fish.
A chromatographic procedure using sequential ion-exchange columns is described for separating choline, trimethylamine, trimethylamine oxide, and betaine extracted from marine fish tissues; added exogenous carnitine can also be separated by the system. Choline with its positive charge binds to the AG 50W-X8 (Na+, pH 9) column. The column is first eluted with 0.1 N NaOH to collect trimethylamine, trimethylamine oxide, and betaine; choline is then eluted with 0.5 N NaOH. The amines collected with 0.1 N NaOH are subsequently separated using an AG 50W-X8 (H+, pH 4) column eluted with a linear 0-1 M NaC1 gradient.
D000818:Animals; D001622:Betaine / Q000302:isolation & purification*; D002331:Carnitine / Q000032:analysis; D002794:Choline / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange; D005399:Fishes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008744:Methylamines / Q000302:isolation & purification*; D009132:Muscles / Q000378:metabolism; D014020:Tissue Extracts / Q000032:analysis
D008744:Methylamines; D014020:Tissue Extracts; D001622:Betaine; C005855:trimethyloxamine; C023336:trimethylamine; D002794:Choline; D002331:Carnitine