Propagation of human candidate calicivirus in cell culture.
Evidence is presented for the first time that a human candidate calicivirus (HCV) replicates in human embryo kidney cells when trypsin is incorporated in the culture medium. The virus multiplies in the presence of actinomycin D and radiolabelling experiments with [3H]uridine indicate that it has an RNA genome. These observations provide further support for the view that HCV should be tentatively classified as a member of the Caliciviridae .
D002139:Caliciviridae / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D003609:Dactinomycin / Q000494:pharmacology; D005243:Feces / Q000382:microbiology; D005814:Genes, Viral; D006801:Humans; D007668:Kidney / Q000382:microbiology; D008854:Microscopy, Electron; D012367:RNA, Viral / Q000235:genetics; D014357:Trypsin / Q000494:pharmacology; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects
D012367:RNA, Viral; D003609:Dactinomycin; D014357:Trypsin