title: The nucleotide sequence of bacteriophage T5 glutamine transfer RNA.
abstract: Uniformly 32P-labeled phage-specific tRNAGln has been isolated from bacteriophage T5-infected Escherichia coli cells and its nucleotide sequence has been determined using thin-layer chromatography on cellulose to fractionate the oligonucleotides. The sequence is: pUGGGGAUUAGCUUAGCUUGGCCUAAAGCUUCGGCCUUUGAAG psi CGAGAUCAUUGGT psi CAAAUCCAAUAUCCCCUGCCAOH. The main feature of this tRNA is the absence of Watson-Crick pairing between the 5'-terminal base and the fifth base from its 3'-end. The structure of tRNA was confirmed by DNA sequencing of its gene.
mesh_terms: D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D005973:Glutamine; D009690:Nucleic Acid Conformation; D012343:RNA, Transfer; D013604:T-Phages / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D005973:Glutamine; D012343:RNA, Transfer