Ophthalmic and norophthalmic acid in lens, liver, and brain of higher animals.
A sensitive high-performance liquid chromatographic method for the determination of nanomole levels of ophthalmic and norophthalmic acid has been described. The procedure is based upon the conversion of amino group to 2,4-dinitrophenyl derivatives and the detection at 420 nm. This method was applied to the determination of the peptides in lens, liver, and brain of several animals. Mean recoveries were 93.6% for ophthalmic acid and 92.3% for norophthalmic acid added to cattle lens homogenate.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D006168:Guinea Pigs; D006801:Humans; D007908:Lens, Crystalline / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D009842:Oligopeptides / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D013552:Swine
D009842:Oligopeptides; C041021:norophthalamic acid; C016632:ophthalmic acid