title: Liquid chromatographic analysis of promethazine and its major metabolites in human postmortem material.
abstract: A method is described for the determination of promethazine and some of its major metabolites in postmortem specimens by enzymic digestion followed by high-pressure liquid chromatography of the extracts using a cyano-bonded (mu-Bondapak-CN) silica column packing and a mobile phase consisting of methanol, water, and n-octylamine (adjusted to pH 8). The system will separate and quantitate promethazine sulphoxide, desmonomethyl promethazine, didesmethyl promethazine, and promethazine. Pericyazine (2-cyano-10-[3-(4- hydroxypiperidino )propyl] phenothiazine) was used as internal standard. The parent drug and metabolites were extracted from a enzyme digested tissue homogenates with ethyl acetate using a simple, single micro-extraction procedure. The method was applied to four cases of fatal poisoning involving promethazine ingestion.
mesh_terms: D000328:Adult; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D011398:Promethazine / Q000032:analysis* / Q000378:metabolism; D019966:Substance-Related Disorders
chemical_list: D011398:Promethazine