Endotoxin-induced inhibition of steroid binding by mouse liver cytosol.
Glucocorticoids have been reported to ameliorate the lethal effects of endotoxin in a wide variety of animals, including man. Restoration of hepatic gluconeogenic enzymes and nucleotides may be involved in this effect. In this study decreased binding of 3H-dexamethasone was observed in liver cytosol preparations obtained from adrenalectomized C3HeB/FeJ mice treated with 100 micrograms of Escherichia coli 0111:B4 endotoxin (Boivin). Adrenalectomy significantly reduced th endotoxin LD50 value from 4.7 mg/kg to 2.3 micrograms/kg. The amount of labeled steroid bound by endotoxin-treated mice was 13,606 +/- 2,027 dpm/mg cytosol protein compared with 17,247 +/- 2,084 dpm/mg cytosol protein in adrenalectomized controls. Results from studies on the distribution of 14C-endotoxin suggested the inhibition of steroid binding seen in liver may be a mediated event. It is likely perturbations in steroid action at the subcellular/molecular level are involved.
D000315:Adrenalectomy; D000818:Animals; D001798:Blood Proteins / Q000096:biosynthesis*; D003600:Cytosol / Q000378:metabolism*; D003907:Dexamethasone / Q000378:metabolism; D004731:Endotoxins / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D004926:Escherichia coli; D005938:Glucocorticoids / Q000037:antagonists & inhibitors*; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D013997:Time Factors
D001798:Blood Proteins; D004731:Endotoxins; D005938:Glucocorticoids; D011965:Receptors, Glucocorticoid; D003907:Dexamethasone