Isolation, partial identification and quantitative determination of four guaiphenesin glucuronides in plasma and urine of the horse by high-performance liquid chromatography.
The isolation, partial identification and quantitative determination of four guaiphenesin glucuronides in plasma and urine of the horse is described. The identity of the glucuronides was checked by UV and fluorescence spectrophotometry, by NMR spectrometry and by mass spectrometry after permethylation. The applicability of the procedure to pharmacokinetic studies is demonstrated.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005965:Glucuronates / Q000097:blood* / Q000652:urine; D006140:Guaifenesin / Q000097:blood* / Q000652:urine; D006736:Horses; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D005965:Glucuronates; D006140:Guaifenesin