Effect of N-methylation of phosphatidylethanolamine on the fluidity of phospholipid bilayers.
The effect of N-methylphosphatidylethanolamine on phase transition and the fluidity of the liposomes made of dipalmitoylphosphatidylcholine or phosphatidylethanolamine was studied by the steady-state fluorescence polarization method and differential scanning calorimetry. N-methylation of phosphatidylethanolamine caused a decrease of fluidity of liposomes made of dipalmitoylphosphatidylcholine, but had little effect on dipalmitoylphosphatidylethanolamine. The liposomes prepared with both phosphatidylcholine and N-methylphosphatidylethanolamine and also phosphatidylethanolamine and N-methylphosphatidylethanolamine could be composed of solid solution and exhibited symmetric phase diagram.
D000818:Animals; D002152:Calorimetry, Differential Scanning; D005454:Fluorescence Polarization; D007942:Leukemia, Experimental / Q000378:metabolism; D008051:Lipid Bilayers; D008081:Liposomes / Q000378:metabolism; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000378:metabolism*; D008745:Methylation; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D051381:Rats; D013816:Thermodynamics
D008051:Lipid Bilayers; D008081:Liposomes; D008563:Membrane Lipids; D010714:Phosphatidylethanolamines