Cell-mediated and humoral immune responses in guinea pigs sensitized with the thermostable antigen of human granulocytes (TSGA).
Cell-mediated and humoral immune responses are induced shortly after a single injection of the human thermostable granulocyte antigen TSGA to guinea pigs. This was proved by means of the lymphocyte transformation assay and the estimation of MIF and LIF activity in supernatants of antigen-stimulated lymph node cells as well as by immunodiffusion, immunoelectrophoresis and immunohistology. The maximum of the cellular response was observed 10 days after immunization. The antibody production appeared after a lag time and increased continuously thereafter. The antisera of guinea pig yields a strong specific fluorescence in neutrophils and a weak fluorescence in monocytes of man. The humoral immune responses of guinea pig antisera correspond to the results obtained with antisera from rabbits.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D006098:Granulocytes / Q000276:immunology*; D006168:Guinea Pigs; D006358:Hot Temperature; D006801:Humans; D007109:Immunity; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis; D008297:Male; D013997:Time Factors
D000941:Antigens; D007961:Leukocyte Migration-Inhibitory Factors; D008263:Macrophage Migration-Inhibitory Factors