Single step purification of locust adipokinetic hormones I and II by reversed-phase high-performance liquid chromatography, and amino-acid composition of the hormone II.
Locust adipokinetic hormones I (AKH I) and II (AKH II) are separated by reversed-phase high-performance liquid chromatography using a mu-Bondapak phenyl column with a trifluoroacetic acid/acetonitrile gradient. The eluant was monitored at 210 nm and the hyperlipaemic activity was detected using a bioassay. The amino-acid composition of AKH II was determined after acid hydrolysis with HCl or methane-sulfonic acid. It contained the following amino-acid residues in almost equimolar amounts: Asp, Ser, Glu, Gly, Ala, Leu, Phe and Trp.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006110:Grasshoppers / Q000032:analysis*; D006458:Hemolymph / Q000032:analysis; D007301:Insect Hormones / Q000302:isolation & purification*
D000596:Amino Acids; D007301:Insect Hormones; C014956:adipokinetic hormone (locust)