title: [Participation of chromatin proteins in the binding of glucocorticoid receptor complexes by the cell nucleus].
abstract: The binding of the purified glucocorticoid-receptor complexes from rat liver cytosol with partially deproteinized and partially reconstituted chromatins from rats liver and brain has been investigated. The chromatin was deproteinized by stepwise extraction of nuclei with NaC1 solution of raised molarity. The partial chromatin reconstruction was accomplished by the addition of individual fractions of extracted proteins to DNA-cellulose. It has been shown that in both test-systems used some fractions of nuclear proteins in particular the fractions of loosely associated with chromatin nonhistone proteins are capable of modulating specifically (mainly positively) the binding of glucocorticoid-receptor complexes with chromatin.
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D002843:Chromatin / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D008099:Liver / Q000378:metabolism; D009698:Nucleoproteins / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000302:isolation & purification / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*
chemical_list: D002843:Chromatin; D009698:Nucleoproteins; D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid
vernacular_title: Uchastie belkov khromatina v sviazyvanii gliukokortikoid-retseptornykh kompleksov kletochnym iadrom.