Adenovirus DNA replication in vitro is stimulated by RNA from uninfected HeLa cells.
Adenovirus DNA replication was studied in a partially reconstituted system consisting of purified viral proteins (DNA-binding protein, precursor terminal protein and Ad DNA polymerase) and a nuclear extract from uninfected HeLa cells. Optimal DNA replication required the presence of a heat-stable, ribonuclease-sensitive fraction from the cytosol of uninfected cells. This fraction stimulated the initiation about 3-fold and the replication of origin fragments 5-10-fold. Sedimentation analysis indicated the presence of a fast-sedimenting and a slow-sedimenting component which complemented each other. At least part of the stimulation was caused by low-molecular-mass RNA.
D000260:Adenoviruses, Human / Q000235:genetics*; D003600:Cytosol / Q000502:physiology; D004261:DNA Replication; D004279:DNA, Viral / Q000302:isolation & purification; D006367:HeLa Cells / Q000502:physiology; D006801:Humans; D007700:Kinetics; D012334:RNA, Neoplasm / Q000302:isolation & purification / Q000378:metabolism*
D004279:DNA, Viral; D012334:RNA, Neoplasm