A native whole blood test for the evaluation of blood-surface interaction: determination of thromboxane production.
The method developed to evaluate the hemocompatibility of artificial materials involves the determination of thromboxane production during the clotting of rabbit blood, in test tubes of different materials. The concentration of serum TXB2 obtained after incubation of whole blood in glass test tubes, for 40 min at 37 degrees C, averaged 416.8 +/- 23.3 ng/ml (mean +/- SE). Polymethylpentene, recognised as having a relatively poor blood compatibility, elicited 309.5 +/- 17.2 ng/ml of serum TXB2, while silicone and Avcothane, considered of better hemocompatibility, showed thromboxane levels of 276.2 +/- 28.2 and 222.9 +/- 31.5 ng/ml, respectively. These values validate the usefulness of the proposed method as a preliminary in vitro screening test of artificial materials intended for biomedical application.
D000818:Animals; D001672:Biocompatible Materials; D001777:Blood Coagulation; D005898:Glass; D008297:Male; D011090:Polyenes; D011140:Polyurethanes; D011817:Rabbits; D012826:Silicone Elastomers; D012828:Silicones; D013499:Surface Properties; D013928:Thromboxane A2 / Q000097:blood*; D013931:Thromboxanes / Q000097:blood*
D001672:Biocompatible Materials; D011090:Polyenes; D011140:Polyurethanes; D012826:Silicone Elastomers; D012828:Silicones; D013931:Thromboxanes; C000144:Avcothane; D013928:Thromboxane A2; C021245:poly(methylpentene)