A radioenzymatic method for S-adenosyl-L-methionine determination in biological fluids.
Current methods for the radioenzymatic assay of S-adenosyl-L-methionine (AdoMet) in biological fluids have been modified in order to increase sensitivity. The modified procedure has allowed to measure AdoMet content also in plasma and cerebrospinal fluid where the concentrations have been found to range between 17 and 72 ng/ml in the different animal species.
D000121:Acetylserotonin O-Methyltransferase; D000818:Animals; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D006801:Humans; D008550:Melatonin; D008780:Methyltransferases; D011817:Rabbits; D011854:Radiochemistry / Q000379:methods; D051381:Rats; D012436:S-Adenosylmethionine / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D012701:Serotonin / Q000031:analogs & derivatives / Q000138:chemical synthesis; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; D012701:Serotonin; D012436:S-Adenosylmethionine; D008780:Methyltransferases; D000121:Acetylserotonin O-Methyltransferase; D008550:Melatonin; C006389:N-acetylserotonin