Biological activities of broad bean (Vicia faba L.) extracts cultivated in South Anatolia in favism sensitive subjects.
Aqueous extracts of a different variety of fresh broad bean seeds obtained from a favism endemic area in Turkey, were incubated with blood from sensitive and non-sensitive (control) subjects. Red blood cells were characterized by a whole blood glutathione (GSH) and a deficiency of Glucose-6-phosphate dehydrogenase (G-6-PD) activity. As the decrease in GSH percent is taken as an index of haemolytic activity, the test results were as following: Sakiz , Milas -Region, French broad bean extracts reduced the blood GSH levels 48%, 70%, 46% and 53%, respectively, in favism sensitive subjects. Active principles which are responsible for the haemolysis ( Vicine and Convicine ) were isolated from broad beans and their effects on GSH levels of blood were 99% and 81%, respectively, in favism sensitive subjects and 33.3% and 19% in normal subjects.
D007887:Fabaceae / Q000032:analysis / Q000633:toxicity*; D005236:Favism / Q000097:blood*; D005960:Glucosides / Q000302:isolation & purification; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D010936:Plant Extracts / Q000633:toxicity*; D010946:Plants, Medicinal; D011744:Pyrimidinones / Q000302:isolation & purification; D014421:Turkey; D014498:Uracil / Q000031:analogs & derivatives / Q000302:isolation & purification; D014529:Uridine / Q000031:analogs & derivatives*
D005960:Glucosides; D010936:Plant Extracts; D011744:Pyrimidinones; D014498:Uracil; C007116:convicine; C009661:vicine; D014529:Uridine