title: Chromosomal aberrations and SCEs in Allium cepa root-tip cells treated with caffeine and pyronin Y.
abstract: The effectiveness of caffeine and pyronin Y in the induction of both chromosomal aberrations and sister-chromatid exchanges (SCEs) in root meristematic cells of A. cepa was studied. The rate of SCEs proved to be increased when 5-bromo-2'-deoxyuridine- (BrdU) substituted chromosomes were allowed to replicate in thymidine (dT) for a second S period simultaneously with caffeine or pyronin Y. In contrast, only caffeine was able to induce aberrations in BrdU-substituted chromosomes, while pyronin Y seemed to be ineffective at the doses employed.
mesh_terms: D001973:Bromodeoxyuridine / Q000378:metabolism; D002110:Caffeine / Q000494:pharmacology*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003434:Crossing Over, Genetic / Q000187:drug effects*; D004247:DNA / Q000235:genetics; D004260:DNA Repair; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects*; D010944:Plants; D011754:Pyronine / Q000494:pharmacology*; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D014966:Xanthenes / Q000494:pharmacology*
chemical_list: D014966:Xanthenes; D002110:Caffeine; D004247:DNA; D001973:Bromodeoxyuridine; D011754:Pyronine