title: Alternative fluorochromes to ethidium bromide for automated read out of cytotoxicity tests.
abstract: Propidium iodide (PI) and diamidinophenylindol (DAPI) were compared with ethidium bromide (EB) in cytotoxicity tests. Double blind studies showed similar results with the 3 dyes in terms of percentages of dead (fluorescing) cells. The dyes were also investigated for their potential combination with the immunofluorescent membrane markers FITC and TRITC. Both EB-FITC and PI-FITC are suitable for conventional HLA-DR typing procedures. Because of its spectral properties, the DAPI-TRITC combination is promising for automated read out of DR-typing tests by image analysis systems.
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D004996:Ethidium / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000494:pharmacology*; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D008214:Lymphocytes / Q000378:metabolism; D011419:Propidium / Q000494:pharmacology
chemical_list: D005456:Fluorescent Dyes; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D007211:Indoles; D011419:Propidium; C007293:DAPI; D004996:Ethidium