Temperature and pH dependence of nerve growth factor-mediated neurite outgrowth of PC12 pheochromocytoma cells.
Nerve growth factor-mediated neurite outgrowth of PC12 pheochromocytoma cells was dependent on medium pH and temperature. Optimal pH was 6.8-7.1. No neurites were formed below 25 degrees C, and the number of cells having neurites increased upon elevating temperature. In contrast, the cells pretreated with nerve growth factor in suspension culture developed neurites even at 25 degrees C when they were transferred to monolayer culture. Temperature dependence of rates of the neurite formation indicates that apparent activation energy for this process is 44.6 kJ/mol.
D000310:Adrenal Gland Neoplasms / Q000503:physiopathology*; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D051379:Mice; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010673:Pheochromocytoma / Q000503:physiopathology*; D013696:Temperature
D009414:Nerve Growth Factors