Revised method for the quantitative determination of 5-hydroxytryptamine in human plasma by gas chromatography--mass spectrometry--selected ion monitoring.
To estimate the 5-hydroxytryptamine level in human plasma by gas chromatography--mass spectrometry--selected ion monitoring (GC--MS--SIM), a recovery method from plasma and derivatization conditions with pentafluoropropionic anhydride were investigated. By heating 5-hydroxytryptamine at 140 degrees C for 4 h in the presence of pentafluoropropionic anhydride the peak intensity of derivatized 5-hydroxytryptamine increased more than three times in comparison with the reported method. There was a marked difference in the plasma levels of 5-hydroxytryptamine obtained using the GC--MS--SIM method compared to those obtained using fluorometric assay.
D002250:Carbon Radioisotopes; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D012701:Serotonin / Q000097:blood*; D013050:Spectrometry, Fluorescence / Q000379:methods
D002250:Carbon Radioisotopes; D012701:Serotonin