Determination of the order of gene function in the yeast nuclear division pathway using cs and ts mutants.
Cold-sensitive (cs) and heat-sensitive (ts) conditional-lethal mutations that affect specifically the cell division cycle of budding yeast (Saccharomyces cerevisiae) were used to determine the order of gene function. Reciprocal temperature-shift experiments using cs-ts double mutants revealed a detailed order of function among genes whose execution points and mutant phenotypes are very similar. The data suggest that the nuclear branch of the overall cell-cycle pathway itself contains at least one branch.
D002453:Cell Cycle; D002467:Cell Nucleus / Q000502:physiology*; D004261:DNA Replication / Q000187:drug effects; D004271:DNA, Fungal / Q000235:genetics; D006918:Hydroxyurea / Q000494:pharmacology; D007700:Kinetics; D009154:Mutation; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000502:physiology
D004271:DNA, Fungal; D006918:Hydroxyurea