Distribution and some properties of bile salt-binding proteins in rainbow trout, Salmo gairdneri.
Trout liver cytosol has proteins that bind bile salts and 8-anilino-1-naphthalene sulphonate and that co-elute from Sephadex G-75. The binding of 8-anilino-1-naphthalene sulphonate is decreased by 90% in the presence of 33 microM lithocholate, implying the proteins are the same. Bile salt-binding protein is present in liver, but was not detected in gills, kidney, muscle or intestine. Bile salt-binding protein, partially-purified by Sephadex G-75 chromatography, was resolved into 2 fractions by cholate-affinity chromatography. Fatty acids do not appear to be bound by bile salt-binding protein. No fatty acid-binding protein was detected in trout liver cytosol.
D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003600:Cytosol / Q000378:metabolism; D006913:Hydroxysteroid Dehydrogenases; D007668:Kidney / Q000378:metabolism*; D008095:Lithocholic Acid / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins; D009132:Muscles / Q000378:metabolism*; D010169:Palmitic Acids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D014337:Trout; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)
D002352:Carrier Proteins; D006913:Hydroxysteroid Dehydrogenases; D008095:Lithocholic Acid; D008562:Membrane Glycoproteins; D010169:Palmitic Acids; C037643:bile acid binding proteins; C435496:AKR1C2 protein, human; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)