title: Hyperosmotic arabinose solutions open the tight junctions between brain capillary endothelial cells in tissue culture.
abstract: Tight junctions between bovine brain capillary endothelial cells in primary cell culture are impermeable to horseradish peroxidase (HRP) after 5 min of incubation. However, following 1-5 min of exposure to 1.6 M arabinose, HRP penetrates the extracellular space between successive tight junctions. Endothelial cells in control cultures contain a small number of cytoplasmic pits and vesicles containing HRP which do not increase in number after hyperosmotic treatment.
mesh_terms: D000818:Animals; D001089:Arabinose / Q000494:pharmacology*; D001812:Blood-Brain Barrier / Q000187:drug effects*; D002199:Capillary Permeability / Q000187:drug effects*; D002417:Cattle; D046508:Culture Techniques; D004727:Endothelium / Q000187:drug effects; D006735:Horseradish Peroxidase / Q000378:metabolism; D006982:Hypertonic Solutions; D007365:Intercellular Junctions / Q000187:drug effects*; D008854:Microscopy, Electron
chemical_list: D006982:Hypertonic Solutions; D001089:Arabinose; D006735:Horseradish Peroxidase