Decay of granulocyte adherence following isolation from human blood.
We studied the rate of decay of granulocyte adherence following isolation from human blood using the nylon-fibre microcolumn technique recently developed in our laboratory. The decay in adherence was found to be evident by 6 h, pronounced by 24 h, and very substantial by 48 h. This decay in adherence can be reversed by incubation at 4 degrees C instead of 37 degrees C. It can also be retarded by incubation with both superoxide dismutase and 2,3-dihydroxybenzoic acid. The data suggests that peroxidation of the granulocyte membrane is a possible mechanism for the decay in granulocyte adherence. These results provide further insight into biological aspects of granulocyte activity, and have implications for the laboratory evaluation of granulocyte function.
D002448:Cell Adhesion / Q000187:drug effects; D006098:Granulocytes / Q000502:physiology*; D006801:Humans; D062385:Hydroxybenzoates / Q000494:pharmacology; D066298:In Vitro Techniques; D013482:Superoxide Dismutase / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
D062385:Hydroxybenzoates; C009135:2,3-dihydroxybenzoic acid; D013482:Superoxide Dismutase