title: Attachment of origins of replication to the nuclear matrix and the chromosomal scaffold.
abstract: We have investigated the attachment of DNA to the nuclear matrix and chromosomal scaffold in synchronized bovine liver cells. Label incorporated at the onset of the S phase remained preferentially associated with the matrix during the subsequent G1 phase and with a residual protein structure from dehistonized chromosomes during mitosis. On the other hand label incorporated during mid or late S phase was about equally distributed over the DNA molecule after a chase into the G1 phase. These results suggest that DNA is attached to the nuclear matrix and chromosome scaffolds by the origins of replication.
mesh_terms: D000818:Animals; D002417:Cattle; D002453:Cell Cycle; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D002875:Chromosomes / Q000502:physiology*; D004247:DNA / Q000096:biosynthesis / Q000302:isolation & purification; D004261:DNA Replication; D007700:Kinetics; D008099:Liver / Q000378:metabolism*
chemical_list: D004247:DNA