Simplified determination of carboxyhemoglobin.
This spectrophotometric method for estimating carboxyhemoglobin is based on the production of a two-pigment mixture by reducing oxyhemoglobin and methemoglobin with sodium hydrosulfite . Absorbances of the pigment are measured at 420 and 432 nm. Protecting the solutions against exposure to air is unnecessary. Values are stable and reproducible when the solutions are buffered at pH 6.85.
D002263:Carboxyhemoglobin / Q000032:analysis*; D004227:Dithionite; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D008433:Mathematics; D008706:Methemoglobin; D010084:Oxidation-Reduction; D010108:Oxyhemoglobins; D012907:Smoking; D013048:Specimen Handling; D013053:Spectrophotometry / Q000379:methods; D013435:Sulfhemoglobin
D006454:Hemoglobins; D007202:Indicators and Reagents; D010108:Oxyhemoglobins; D004227:Dithionite; D008706:Methemoglobin; D013435:Sulfhemoglobin; D002263:Carboxyhemoglobin