Transcriptional activity of blastomeres in mouse embryos during delayed implantation and after oestradiol benzoate-induced resumption of development.
The relative activities of endogenous DNA-bound RNA polymerases have been quantified in individual blastocysts of ovariectomized mice during delayed implantation and following oestradiol-induced resumption of development. Incorporation of [3H]UMP into an acid insoluble product was detected in the cell nuclei by autoradiography. Nucleolar polymerase I and the nucleoplasmic polymerase II were present in delayed blastocysts harvested from progesterone- or oil-treated ovariectomized females. However, the activities of these enzymes were considerably reduced in comparison with those of blastocysts of intact mice. A stimulation of transcription appeared to occur 18 h after oestradiol benzoate injection of progesterone-treated females but more rapidly in blastocysts from oil-treated females. The data indicate that progesterone may retard the rate of oestradiol-induced reactivation in delayed blastocysts.
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000502:physiology; D001757:Blastomeres / Q000187:drug effects / Q000502:physiology*; D002369:Castration; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D010064:Embryo Implantation; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects*; D014316:Tritium; D014542:Uridine Monophosphate / Q000378:metabolism
D014316:Tritium; D011374:Progesterone; D004958:Estradiol; D014542:Uridine Monophosphate; D012321:DNA-Directed RNA Polymerases