Stimulation of DNA synthesis by microinjection of diadenosine 5',5''-P1, P4-tetraphosphate (Ap4A) into Xenopus laevis oocytes.
Ap4A stimulated DNA synthesis when injected into oocytes. The stimulation was dramatically increased when an exogenous template was microinjected. Aphidicolin inhibited the effect of Ap4A, supporting a role of DNA polymerase alpha in this process. No stimulation by Ap4A was observed in microinjected eggs, nor ATP was able to mimic the in vivo effect of Ap4A. Besides microinjected activated DNA, the stimulation by Ap4A of DNA synthesis was also observed with poly dT and poly dT-poly dA as templates, while no effect was observed with poly dA- dT12 -18 and poly dC- dG12 -18. These results support a role of Ap4A in the initiation of DNA synthesis.
D000227:Adenine Nucleotides / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D015226:Dinucleoside Phosphates; D005260:Female; D008845:Microinjections; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism; D011071:Poly T / Q000378:metabolism; D011067:Poly dA-dT / Q000378:metabolism; D013698:Templates, Genetic; D014982:Xenopus laevis
D000227:Adenine Nucleotides; D015226:Dinucleoside Phosphates; D011071:Poly T; D011067:Poly dA-dT; C020733:diadenosine tetraphosphate; C011440:diadenosine triphosphate; D000255:Adenosine Triphosphate; D004247:DNA