Human liver mitochondrial aldehyde dehydrogenase: a C-terminal segment positions and defines the structure corresponding to the one reported to differ in the Oriental enzyme variant.
A C-terminal segment of mitochondrial human liver aldehyde dehydrogenase was characterized. The results prove that a central part of this segment largely but not completely agrees with a structure of a tryptic peptide previously reported for the same isoenzyme. This part corresponds to a segment that contains the exchanged residue in the functionally deficient Oriental variant of mitochondrial aldehyde dehydrogenase [(1984) Proc. Natl. Acad. Sci. USA 81, 258-261]. The data suggest important functions for the C-terminal region of aldehyde dehydrogenase, clarify previously inconsistent results, and establish this structure in the typical enzyme, including the position corresponding to the mutation in the functional variant.
D000444:Aldehyde Dehydrogenase; D000445:Aldehyde Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D044466:Asian People; D003488:Cyanogen Bromide; D014644:Genetic Variation; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008930:Mitochondria, Liver / Q000201:enzymology*; D010446:Peptide Fragments / Q000032:analysis; D014357:Trypsin
D007527:Isoenzymes; D010446:Peptide Fragments; D000445:Aldehyde Oxidoreductases; D000444:Aldehyde Dehydrogenase; D014357:Trypsin; D003488:Cyanogen Bromide