A rapid, high-yield method for the purification of the water-soluble 33 kilodalton protein of spinach thylakoids.
A new purification procedure for the water-soluble 33 kDa protein of Photosystem II is presented. The method is based on the selective release of the 33 kDa protein at slightly elevated temperatures and involves a minimum of purification steps. Starting with spinach leaves, the pure protein may be obtained in about 4 h, with a yield usually higher than 60%.
D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002736:Chloroplasts / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D010788:Photosynthesis; D010940:Plant Proteins / Q000302:isolation & purification*; D012995:Solubility; D014867:Water
D000596:Amino Acids; D010940:Plant Proteins; D014867:Water