title: Steady-state cultures of human skin.
abstract: Pretransplantation cultivation of adult human skin has been optimized for rapid and prolonged outgrowth of epidermal cells from tissue explants using autonomic-perfusion, thin-layer culture technology (steady-state). This system fostered growth of autologous mesenchymal elements via critical control of the culture environment. The resulting cellular outgrowth maintained a balanced epithelial-dermal relationship, contained keratinocytes as well as minority epidermal cells, melanocytes and possibly Langerhans cells. Critical control of culture pH and osmolarity was found to enhance epithelial cell proliferation.
mesh_terms: D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D046508:Culture Techniques; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D009994:Osmolar Concentration; D012867:Skin / Q000166:cytology*; D016038:Skin Transplantation
chemical_list: D003470:Culture Media