[Mechanism of the Crabtree effect: influence of pH on the rate of mitochondrial respiration].
A detailed study of dependence of mitochondrial respiration on pH of incubation medium has been carried out. It was shown that during oxidation of malic, succinic and ascorbic acids mitochondrial respiration shows a sharp fall, when the pH of incubation medium is decreased from 7.5 to 6.5. It was assumed that one of the possible causes of the Crabtree effect is a reduction of the pH value of the cytoplasm.
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D001204:Ascorbate Oxidase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008930:Mitochondria, Liver / Q000378:metabolism*; D010101:Oxygen Consumption; D051381:Rats; D013385:Succinate Dehydrogenase / Q000378:metabolism
C021526:malate oxidase; D000429:Alcohol Oxidoreductases; D001204:Ascorbate Oxidase; D013385:Succinate Dehydrogenase
O mekhanizme éffekta krebtri: vliianie pH sredy inkubatsii na skorost' dykhaniia mitokhondriĭ.