Effect of diet on the queuosine family of tRNAs of germ-free mice.
The transfer RNAs for aspartic acid, asparagine, histidine, and tyrosine respond to codons in the third column of the genetic code and contain a hypermodified nucleoside known as queuosine (Q) in the first position of the anticodon of the major isoacceptor tRNA. Nothing is known about the physiological or biochemical function of Q. Germ-free mice were maintained for a period of nine tRNA half-lives on a chemically defined diet known to contain all essential constituents of the rodent diet but no Q or its base, queuine. The tRNAs for histidine and asparagine contained only 15% of the Q-containing isoacceptor tRNA. On the other hand, the Q-containing isoacceptor comprised 88% of the tRNAHis and 85% of the tRNAAsn in conventional mice and germ-free mice fed commercial mouse chow. Transfer RNAAsp and tRNATyr were completely modified with respect to Q in germ-free mice maintained on the chemically defined diet as well as on normal mouse chow. Germ-free mice fed the chemically defined diet contained normal amounts of the hypermodified base wye in tRNAPhe.
D000818:Animals; D000926:Anticodon; D001216:Asparagine; D001224:Aspartic Acid; D003062:Codon; D004032:Diet; D005260:Female; D005856:Germ-Free Life; D006151:Guanosine / Q000031:analogs & derivatives*; D006639:Histidine; D008297:Male; D051379:Mice; D009704:Nucleoside Q / Q000032:analysis*; D012343:RNA, Transfer / Q000378:metabolism*; D014443:Tyrosine
D000926:Anticodon; D003062:Codon; D006151:Guanosine; D001224:Aspartic Acid; D014443:Tyrosine; D006639:Histidine; D009704:Nucleoside Q; D001216:Asparagine; D012343:RNA, Transfer