Production by cultured spleen cells of inflammatory substances and other lymphokines that mediate delayed-type hypersensitivity in mice.
In vitro cultivation of normal mouse spleen cells with human serum albumin generated effector cells that mediate the delayed-type hypersensitivity (DTH) reaction. The cultured cells, when incubated in a serum-free medium for a further 24 hr, released substances (FPRF) which caused a footpad inflammatory reaction at a maximum of 6 hr after injection into normal syngeneic or allogeneic strains of mice, as well as macrophage migration inhibition factor (MIF) and macrophage activating factor (MAF). The DTH-effector cells in the culture were fractionated in the low density layers by discontinuous bovine serum albumin density gradient centrifugation. The effector cells in the low density layers were further enriched in the Lyt 1 subpopulation of T cells when fractionated on a fluorescence activated cell sorter. Cells capable of producing the inflammatory substances (FPRF), MIF and MAF were also enriched in the same fraction containing DTH-effector cells. These results suggest that low density, Lyt 1-positive T cells mediating the DTH reaction produce FPRF as well as MIF and MAF.
D000818:Animals; D000950:Antigens, Ly; D002469:Cell Separation; D002478:Cells, Cultured; D005260:Female; D006968:Hypersensitivity, Delayed / Q000209:etiology*; D007249:Inflammation / Q000209:etiology; D008222:Lymphokines / Q000096:biosynthesis*; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis*; D016215:Macrophage-Activating Factors; D051379:Mice; D008815:Mice, Inbred Strains; D012710:Serum Albumin, Bovine / Q000276:immunology; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D000950:Antigens, Ly; D008222:Lymphokines; D008263:Macrophage Migration-Inhibitory Factors; D016215:Macrophage-Activating Factors; D012710:Serum Albumin, Bovine