Mutants of Escherichia coli K12 sensitive to acidic pH.
Four mutants of Escherichia coli K12 were isolated on the basis of their sensitivity to pH 5.4. Under non-permissive conditions their growth was reversibly inhibited. At pH 7.0 these mutants showed a highly pleiotropic phenotype, which included altered phage and detergent sensitivities and leakage of periplasmic proteins. The findings suggest a defect in the outer membrane, perhaps in lipopolysaccharide. Two mutants mapped in or near the rfa locus, while the other two were removed from this region.
D001435:Bacteriophages; D002874:Chromosome Mapping; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D010641:Phenotype; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014161:Transduction, Genetic
D012967:Sodium Dodecyl Sulfate