[Production of a homogeneous Cl. botulinum type B neurotoxin].
The method for obtaining the neurotoxin, or alpha-fraction of the toxin, of Cl. botulinum, type B, is described. In accordance with this method, the toxin was precipitated three times with hydrochloric acid in the isoelectric zone with subsequent extraction with phosphate (pH 6.8) and citrate-phosphate (pH 5.6) buffers, then fractionated in columns with DEAE cellulose (pH 5.6), DEAE Sephadex A-50 (pH 7.2) and Sephadex G-200 (pH 7.2). The homogeneous neurotoxin preparations with molecular weights ranging from 145,000 to 160,000 and having the isoelectric point at pH 5.5 and toxicity 5.0--10.0 x 10(7) Dlm per 1 mg protein were obtained.
D000818:Animals; D001904:Botulinum Antitoxin / Q000302:isolation & purification; D001905:Botulinum Toxins / Q000302:isolation & purification* / Q000633:toxicity; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D007526:Isoelectric Point; D051379:Mice; D008970:Molecular Weight; D009498:Neurotoxins; D011817:Rabbits
D001904:Botulinum Antitoxin; D009498:Neurotoxins; D001905:Botulinum Toxins
Poluchenie gomogennogo neĭrotksina Cl. botulinum tipa B.