A solid phase immunofluorescent assay for the measurement of salivary immunoglobulin and albumin levels.
A solid phase immunofluorescence assay which can be used to measure immunoglobulin and albumin levels in whole saliva, as well as in duct collected saliva samples, is described. This method is about an order of magnitude more sensitive than the conventional radial immunodiffusion method, allowing its use in situations involving large numbers of samples or restricted sample volumes. The relative precision and accuracy of this method are comparable to those of radial immunodiffusion.
D000293:Adolescent; D000328:Adult; D000418:Albumins / Q000032:analysis*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D008297:Male; D012463:Saliva / Q000276:immunology*; D012471:Salivary Proteins and Peptides / Q000032:analysis*
D000418:Albumins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D012471:Salivary Proteins and Peptides