Direct measure of the destruction of bone marrow cells, after their injection into variously pretreated syngeneic hosts.
125IUdR-labeled bone marrow cells have been injected into syngeneic mice and their destruction measured by counting daily the animals in toto in a gamma counter. In lethally irradiated recipients, the immediate cell loss was the greatest when the irradiation was delivered just prior to the cell transfer and the cellular multiplication in the spleen on day 7, the smallest. The slopes of the destruction between days 1 and 4 are closely comparable, whether the animals were irradiated 45, 21, or 4 hr before the cell transfer. The immediate cell loss was greater in untreated animals than in the cyclophosphamide-treated recipients and the slope of the destruction curve was higher in cyclophosphamide-treated than in untreated animals. The intensity of the restoration measured by the 125IUdR and 59Fe incorporation on day 6 is inversely correlated to the intensity of the early destruction.
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002470:Cell Survival / Q000528:radiation effects; D003520:Cyclophosphamide / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011300:Preoperative Care; D011828:Radiation Chimera; D013154:Spleen / Q000378:metabolism
D003520:Cyclophosphamide