An investigation of some genetic toxicological effects of the fungicide benomyl.
The widely used fungicide benomyl (methyl-1-(butylcarbamoyl)-2-benzimidazole carbamate) and its breakdown product methyl-2-benzimidazole carbamate (MBC) have been reported to have mutagenic activity in some organisms. In experiments with Drosophila melanogaster we found (i) there was no significant increase in recessive lethal frequency after feeding adult flies with Benlate did not increase chromosome breakage or loss significantly; (ii) there was a relatively high incidence of sterility when males of one strain (Oregon R) were fed Benlate or MBC. In experiments in which cultures of human lymphocytes were exposed in vitro to 0.5 mg/ml MBC we observed extreme contraction of the chromosomes but found no evidence of an increase in the number of cells with chromosome aberrations. We conclude that benomyl and MBC are unlikely to be strong mutagens, but more research is needed to exclude the possibility that they are capable of inducing genetic damage in the germ cells of higher organisms.
D000818:Animals; D001542:Benomyl / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology; D002219:Carbamates / Q000494:pharmacology*; D002869:Chromosome Aberrations; D004331:Drosophila melanogaster / Q000187:drug effects; D005260:Female; D005804:Genes, Lethal / Q000187:drug effects; D005808:Genes, Recessive / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D009153:Mutagens; D012730:Sex Chromosomes
D001562:Benzimidazoles; D002219:Carbamates; D009153:Mutagens; C006698:carbendazim; D001542:Benomyl