Enzyme-linked immunosorbent assay for the indirect anti-human globulin test.
An enzyme-linked immunosorbent assay has been developed for detecting antibodies combined with red cell surface antigens. In the test, the fluorescence released from 4-methylumbelliferylphosphate is measured after exposure of antibody-coated red cells to alkaline phosphatase coupled to anti-human globulin. The assay is sensitive, reproducible, quantitative and potentially adaptable to an automatic analyzer system.
D000469:Alkaline Phosphatase / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000276:immunology; D003298:Coombs Test / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004912:Erythrocytes / Q000276:immunology; D005453:Fluorescence; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D007164:Immunosorbents; D011817:Rabbits; D012712:Serum Globulins / Q000276:immunology / Q000302:isolation & purification*
D000906:Antibodies; D007164:Immunosorbents; D012712:Serum Globulins; D000469:Alkaline Phosphatase