Immunological detection and quantification of the reaction products of 2-acetylaminofluorene with guanine in DNA.
Rabbits were immunized with N-(guanosin-8-yl)-N-acetyl-2-aminofluorene bound to bovine serum albumin. The presence of specific antibodies was determined in a radioimmunoassay by competing N-([5'-3H]-guanosin-8-yl)-N-acetyl-2-aminofluorene (specific radioactivity, 25 Ci/mmol) as tracer against various carcinogen-modified and normal DNA components. Highly specific antibodies were obtained (affinity constant, 6.0 X 10(9) l/mol) and have been employed in a sensitive radioimmunoassay to determine N-(deoxyguanosin-8-yl)-N-acetyl-2-aminofluorene directly in enzymatic hydrolysates of DNA modified with N-acetoxy-N-acetyl-2-aminofluorene. In a similar fashion, the modified nucleoside could be detected in hydrolysed rat liver DNA following administration of N-acetyl-2-aminofluorene, with a detection limit of one carcinogen-modified deoxyguanosine moiety per 10(6) deoxynucleotides in 150 micrograms of DNA.
D015073:2-Acetylaminofluorene / Q000276:immunology; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000276:immunology; D006147:Guanine / Q000276:immunology; D007158:Immunologic Techniques
D006147:Guanine; D004247:DNA; D015073:2-Acetylaminofluorene