Islet-activating protein (IAP) reduces bethanechol-stimulated release of pancreatic polypeptide in the dog.
The effect of islet-activating protein (IAP) purified from culture medium of Bordetella pertussis was examined in dogs. This was assessed by the levels of pancreatic polypeptide (PP) as well as the responses of plasma insulin and glucagon to a parasympathomimetic agent, bethanechol. Plasma responses of these pancreatic hormones were measured before and 5 days after IAP injection. Although IAP had no significant effect on the bethanechol-stimulated increase in plasma glucose, insulin and glucagon, the PP response to bethanechol was significantly reduced after IAP treatment compared with that before IAP (p less than 0.05). In conclusion, IAP significantly and selectively reduced bethanechol-stimulated PP release in the dog although the mechanism remained to be elucidated.
D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology*; D001626:Bethanechol Compounds / Q000494:pharmacology*; D001786:Blood Glucose / Q000032:analysis; D004285:Dogs; D005260:Female; D007328:Insulin / Q000097:blood; D007515:Islets of Langerhans / Q000187:drug effects*; D008297:Male; D010191:Pancreatic Polypeptide / Q000097:blood*; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella
D001426:Bacterial Proteins; D001626:Bethanechol Compounds; D001786:Blood Glucose; D007328:Insulin; D010566:Virulence Factors, Bordetella; D010191:Pancreatic Polypeptide; D037342:Pertussis Toxin