A method for the study of local IgA production using radial immunodiffusion and thin layer chromatography.
A technique is described for the assessment of local IgA production in external secretions variably contaminated with serum IgA. The combination of total IgA and secretory piece measurements by radial immunodiffusion and 11S and 7S IgA measurements by thin layer gel chromatography provide information concerning local IgA and its association with secretory piece.
D001769:Blood / Q000378:metabolism; D002855:Chromatography, Thin Layer / Q000379:methods*; D005260:Female; D006801:Humans; D005779:Immunodiffusion / Q000379:methods*; D007070:Immunoglobulin A / Q000096:biosynthesis*; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis*; D008895:Milk, Human / Q000276:immunology
D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory