Preparation of L- and D-[1-14C]lysine by the resolution of DL-[1-14 C]lysine.
L-[1-14C]lysine was prepared using a technique based on the differential solubility of the diastereomeric salts of DL-lysine with L-glutamic acid. D-[1-14C]lysine was prepared by the enzymatic removal of residual L-[1-14C]lysine from the reaction mixture remaining after the preparation of L-[1-14C]lysine. The products were tested with L-lysine decarboxylase and by column chromatography. L-[1-14C]lysine was obtained in 60% yield with a steroisomeric purity of 95.1%, and D-[1-14C]lysine was obtained in a slightly higher yield with a steroisomeric purity of 97.5%.
D002250:Carbon Radioisotopes; D002262:Carboxy-Lyases; D002852:Chromatography, Ion Exchange; D005590:Fractional Precipitation; D008239:Lysine / Q000032:analysis*; D008722:Methods; D012995:Solubility; D013237:Stereoisomerism
D002250:Carbon Radioisotopes; D002262:Carboxy-Lyases; C021520:lysine decarboxylase; D008239:Lysine