Production of Hydrogen peroxide by Lactobacillus acidophilus.
Hydrogen peroxide production in reconstituted skim milk (10%) and low-fat milk by four strains lf Lactobacillus acidophilus was studied at 37 and 4 C. Strains A and B produced little, but strains C and D produced larger amounts, especially if agitated continuously during growth at 37 C or storage at 4 C. Continuous shaking was required at 4 C for C or D (4.0 X 10(8)/ml) to produce sufficient hydrogen peroxide to retard growth of Pseudomonas fragi. Flavin adenine dinucleotide stimulated the oxidation of reduced nicotinamide adenine dinucleotide by dialyzed cell-free extracts of C and D, which indicated that the reduced nicotinamide adenine dinucleotide oxidases of these strains produce hydrogen peroxide as an end product.
D000818:Animals; D006861:Hydrogen Peroxide / Q000096:biosynthesis* / Q000494:pharmacology; D007779:Lactobacillus acidophilus / Q000378:metabolism*; D008892:Milk / Q000382:microbiology; D009243:NAD / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D011549:Pseudomonas / Q000187:drug effects / Q000254:growth & development; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013696:Temperature
D009243:NAD; D006861:Hydrogen Peroxide; D010088:Oxidoreductases