[Immunoenzyme test with whole trophozoites of Toxoplasma gondii].
In a comparative study 151 human sera were tested for IgG and IgM antibodies against Toxoplasma gondii in the Fluorescent-Antibody-Test (FAT) as well as in the Enzyme-Linked Immunosorbent Assay (ELISA) using the same antigen (whole trophozoites of Toxoplasma gondii). A positive correlation was found between the results obtained in FAT and ELISA. The detection of specific IgG antibodies revealed a correlation of 100% and 97.5% in positive and negative sera, respectively. Only 8 out of 111 positive sera showed deviations of more than +/- 1 step within the titre ranges. While detecting specific IgM antibodies a highly significant correlation (99.3%) between FAT and ELISA was obtained for negative sera. 5 positive IgM sera showed titre end-points of 1:64 and 1 serum an end-point titre of 1:256 in FAT as well as in ELISA; however, a comparison was not done because of the small amount of positive IgM sera having been available.
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D014122:Toxoplasma / Q000276:immunology*
D007074:Immunoglobulin G; D007075:Immunoglobulin M
Enzymimmuntest mit ganzen Trophozoiten von Toxoplasma gondii.