Immunological analysis of plasminogen activators from cultured human cancer cells.
Immunological similarities or differences between urokinase and plasminogen activators from 9 lines of cultured human cancer cells with varying degrees of fibrinolytic activity were examined with antibodies against human urokinase. The antibodies completely inhibited the fibrinolytic activity of 4 lines of gastric cancer, 2 lines of lung cancer, 1 line of urinary bladder cancer and 1 line of renal cancer, indicating that the plasminogen activators from these cell lines were immunologically identical to urokinase. In 5 out of these cell lines, immunological identity was also confirmed by double diffusion analysis. The plasminogen activator from 1 line of lung cancer was found to be immunologically dissimilar to urokinase by a neutralization experiment and double diffusion analysis. These findings indicate that there are at least two immunologically distinguishable forms of plasminogen activators from human cancer cells.
D002478:Cells, Cultured; D006801:Humans; D005779:Immunodiffusion; D007680:Kidney Neoplasms / Q000032:analysis; D008175:Lung Neoplasms / Q000032:analysis; D009369:Neoplasms / Q000032:analysis*; D010960:Plasminogen Activators / Q000032:analysis*; D013274:Stomach Neoplasms / Q000032:analysis; D001749:Urinary Bladder Neoplasms / Q000032:analysis; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis
D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator