Mutagenicity and DNA-damaging activity for several pesticides tested with Bacillus subtilis mutants.
The active pure compounds of 4 pesticides were tested for DNA-damaging and mutagenic activity in Bacillus subtilis and Salmonella typhimurium tester strains. Included were zinc ethylenebisdithiocarbamate (dithane), 1,2-dihydropyridazine-3,6-dione (maleic hydrazide), O,O-dimethylphosphorodithioate (malathion), and 1,2-dibromoethane (fumazone). These agents gave either weak or negative mutagenic responses with the Salmonella/microsome tests for mutagenicity, but were all positive when the tester was B. subtilis strain TKJ6321. Of the 4 chemicals, only fumazone required metabolic activation with rat-liver S9 mix. Upon activation, it produced a volatile mutagenic product. Dithane, maleic hydrazide, and malathion were all mutagenic and did not require metabolic activation. Among these agents, dithane was strongly mutagenic while fumazone, maleic hydrazide and malathion were moderately mutagenic. Only dithane gave significant DNA-damaging activity when applied to a battery of repair-deficient B. subtilis mutants. For the chemicals reported, it is concluded that B. subtilis is superior to S. typhimurium in the detection of mutagenic activity. We strongly recommend its use for prescreening procedures in combination with the S. typhimurium testers.
D000818:Animals; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics*; D001711:Biotransformation; D004247:DNA / Q000235:genetics; D004260:DNA Repair; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006846:Hydrocarbons, Halogenated / Q000633:toxicity*; D008294:Malathion / Q000494:pharmacology*; D008300:Maleic Hydrazide / Q000494:pharmacology; D009153:Mutagens; D010575:Pesticides / Q000494:pharmacology*; D011407:Propane / Q000031:analogs & derivatives* / Q000633:toxicity; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013859:Thiocarbamates / Q000633:toxicity*; D015038:Zineb / Q000633:toxicity*
D006846:Hydrocarbons, Halogenated; D009153:Mutagens; D010575:Pesticides; D013859:Thiocarbamates; D004247:DNA; C007318:1,2-dibromo-3-chloropropane; D011407:Propane; D008300:Maleic Hydrazide; D008294:Malathion; D015038:Zineb