A fluorescein-linked immunoabsorbent assay for the detection of antibacterial antibodies in secretions and serum.
A rapid, quantitative solid-phase immunofluorescence assay has been developed to measure antibodies reactive with Streptococcus mutans in saliva and serum. Formalin killed bacteria were adsorbed to cellulose acetate discs and antibodies bound to the antigen-coated immunoabsorbent were detected by use of fluorescein-labeled antibody to human immunoglobulin isotypes. Quantitation was performed by placing the immunoabsorbent discs in a fluorometer. Low levels of naturally occurring antibodies reactive with S. mutans were easily detected in saliva and serum from normal human subjects.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D001774:Blood Chemical Analysis; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D007163:Immunosorbent Techniques; D011817:Rabbits / Q000276:immunology; D012463:Saliva / Q000032:analysis; D013295:Streptococcus mutans / Q000276:immunology
D000907:Antibodies, Bacterial; D005452:Fluoresceins