Effect of D-glucosamine on the functional maturation of cultured B cells of the neonatal rat.
The present study demonstrates the effect of glucosamine on the functional maturation of cultured B cells of the neonatal rat. When B cells had been maintained at a physiological concentration (5.5 mM) of glucose for 7 days, a drop in the stimulatory effect of 16.7 mM glucose on insulin release and biosynthesis was observed together with a reduced insulin content. By contrast, the sensitivity of glucose-induced insulin release was increased after one week of culture with 5.5 mM glucose and 5 mM glucosamine. And both the insulin content and glucose-induced insulin biosynthesis also remained at the same level as observed at the first day of culture with 5.5 mM glucose alone. In summary, it was suggested that glucosamine-supplemented culture may result in the transition of B cells of neonatal rat from a poor glucose sensitivity to adult-type response of insulin release.
D000818:Animals; D002478:Cells, Cultured; D005944:Glucosamine / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D005947:Glucose; D005944:Glucosamine