Chromosome segregation and expression of rat immunoglobulins in rat/mouse hybrid myelomas.
The chromosome segregation of hybrid myelomas from a fusion of rat immunized spleen cells and the mouse myeloma P3-X63-Ag8 has been analysed. Chromosome loss appears to be nonrandom. Most mouse chromosomes are retained. The rat chromosome are preferentially lost but a few--particularly 1-5 and 13--are rarely lost. The specific retention of some rat chromosomes explains the stability of rat Ig expression in mouse-rat hybrids. Correlation of chromosome loss and retention and loss of rat heavy and light chains leads us to propose chromosome 14 as coding for the heavy chain.
D000818:Animals; D000917:Antibody Formation; D002874:Chromosome Mapping; D002999:Clone Cells / Q000276:immunology; D006822:Hybrid Cells / Q000166:cytology*; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007136:Immunoglobulins / Q000235:genetics*; D007621:Karyotyping; D008540:Meiosis; D051379:Mice; D009101:Multiple Myeloma / Q000235:genetics / Q000276:immunology*; D051381:Rats
D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007136:Immunoglobulins