Evidence for a direct effect of bacitracin on cell-mediated insulin degradation in isolated hepatocytes.
In freshly isolated hepatocytes, in which extracellular degradation of insulin was very low, the degradation velocity was first-order with respect to the amount of insulin bound at steady state. The addition of bacitracin decreased the degradation velocity considerably, so that a higher proportion of cell-associated radioactivity remained intact. The results demonstrate that bacitracin affects the mechanism of insulin processing by intact hepatocytes.
D000818:Animals; D001414:Bacitracin / Q000494:pharmacology*; D002469:Cell Separation; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D001414:Bacitracin