[Immunohistochemical observations on tubulin and alpha actinin during mitosis of cultured fibroblasts].
In this work we studied alpha-actinin and tubulin sites in rabbit fibroblasts in culture. Antibodies anti-alpha-actinin were used for indirect PAP-reaction while antibodies anti-tubulin were used for indirect immunofluorescence method. The observations were carried out by light microscopy, phase-contrast and interference-contrast microscopy, with regard to actinin, and by fluorescence microscopy, with regard to tubulin. During the early mitotic phase, alpha-actinin is localized all over the cell membrane of the fibroblasts, forming a sort strong protective cap, while during diacinesis it forms only rings, localizing below the cell membrane and the philopodia. Thus tubulin forms the bundle fibres during mitotic phases.
D000185:Actinin / Q000032:analysis*; D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D008856:Microscopy, Fluorescence; D008938:Mitosis; D009124:Muscle Proteins / Q000032:analysis*; D011817:Rabbits; D014404:Tubulin / Q000032:analysis*
D009124:Muscle Proteins; D014404:Tubulin; D000185:Actinin
Osservazioni immunoistochimiche della tubulina e dell'alpha-actinina durante la mitosi di fibroblasti in cultura.