Presence of angiotensin II in neurons cultured from fetal rat brain.
By means of a specific antiserum to angiotensin II (ANGII), it has been possible to demonstrate the existence of ANGII immunoreactivity in cells cultured from fetal rat brain. Dissociated cells cultured from the whole brains of 20-day-old fetuses were stained according to the peroxidase antiperoxidase method. Immunoreactive ANGII was observed in less than 1% of the total cell population. These cells have been identified as neurons on the basis of morphological criteria. Positively stained neuronal processes with localized concentrations of ANGII immunoreactivity were visualized in association with ANGII-containing soma. The presence of ANGII in cultured brain cells provides an experimental model for investigating the role of angiotensin in the CNS.
D000804:Angiotensin II / Q000032:analysis*; D000818:Animals; D001921:Brain / Q000196:embryology*; D001923:Brain Chemistry; D002478:Cells, Cultured; D005260:Female; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D009474:Neurons / Q000032:analysis*; D011247:Pregnancy; D051381:Rats
D007106:Immune Sera; D000804:Angiotensin II