Immunological reagents for comparisons of DNA polymerase-alpha and DNA polymerase-beta.
Antibodies to homogeneous calf thymus DNA polymerase-beta and calf thymus DNA polymerase-alpha preparations were raised in rabbits. The antiserum against calf thymus DNA polymerase-beta cross-reacts with all vertebrate DNA polymerase-beta preparations tested, but does not cross-react with trypanosome DNA polymerase-beta, DNA polymerase-gamma, terminal transferase, yeast DNA polymerases, and Escherichia coli DNA polymerase I. The antibodies against calf thymus DNA polymerase-alpha cross-react with DNA polymerase-alpha from mouse, human, and chicken, but do not cross-react with DNA polymerase-alpha from sea urchin embryos and Drosophila embryos, DNA polymerase-beta, DNA polymerase-gamma, terminal transferase, yeast DNA polymerases, and E. coli DNA polymerase I.
D000818:Animals; D002417:Cattle; D002642:Chick Embryo; D003429:Cross Reactions; D004256:DNA Polymerase I / Q000032:analysis*; D004257:DNA Polymerase II / Q000032:analysis*; D004258:DNA Polymerase III / Q000032:analysis; D004259:DNA-Directed DNA Polymerase / Q000032:analysis*; D004926:Escherichia coli / Q000201:enzymology; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D007106:Immune Sera; D007118:Immunoassay; D005779:Immunodiffusion; D007739:L Cells / Q000201:enzymology; D051379:Mice; D013045:Species Specificity; D013950:Thymus Gland / Q000201:enzymology
D007106:Immune Sera; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase