Chlorophyll photobleaching and ethane production in dichlorophenyldimethylurea- (DCMU) or paraquat-treated Euglena gracilis cells.
Light dependent (35 Klux) chlorophyll bleaching in autotrophically grown Euglena gracilis cells at slightly acidic pH (6.5-5.4) is stimulated by the photosystem II blockers DCMU and DBMIB (both 10(-5) M) as well as by the autooxidizable photosystem I electron acceptor, paraquat (10(-3) M). Chlorophyll photobleaching is accompanied by the formation of thiobarbituric acid -- sensitive material ("malondialdehyde") and ethane. Both chlorophyll photobleaching and light dependent ethane formation are partially prevented by higher concentrations (10(-4) M) of the autooxidizable photosystem II electron acceptor DBMIB or by sodium bicarbonate (25 mM). In vitro studies with cell free extracts (homogenates) from E. gracilis suggest that alpha-linolenic acid oxidation by excited (reaction center II) chlorophyll represents the driving force for both ethane formation and chlorophyll bleaching. Ethane formation thus appears to be a sensitive and non-destructive "in vivo" marker for both restricted energy dissipation in photosystem II and, conditions yielding reactive oxygen species at the reducing side of photosystem I.
D002734:Chlorophyll / Q000378:metabolism*; D003624:Darkness; D003991:Dibromothymoquinone / Q000494:pharmacology; D004237:Diuron / Q000494:pharmacology*; D004980:Ethane / Q000378:metabolism; D005056:Euglena gracilis / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008027:Light; D010269:Paraquat / Q000494:pharmacology*; D010788:Photosynthesis / Q000187:drug effects*
D002734:Chlorophyll; D003991:Dibromothymoquinone; D004237:Diuron; D004980:Ethane; D010269:Paraquat