A new RNA polymerase and in vitro transcription of the origin of replication from rat mitochondrial DNA.
A new RNA polymerase was found in a rat mitochondrial extract. This enzyme showed strong template preference in vitro for the supercoiled recombinant plasmid consisting of pBR322 and the D-loop region of rat mtDNA carrying the origin of heavy-strand replication. The main products synthesized by the D-loop region were two RNAs of different sizes. Both of these products were light-strand products transcribed from the region upstream from the origin of replication. This specific transcription is discussed in relation to initiation of primer RNA synthesis for heavy-strand replication of rat mtDNA.
D000818:Animals; D004261:DNA Replication; D004272:DNA, Mitochondrial / Q000235:genetics*; D004274:DNA, Recombinant / Q000378:metabolism; D004278:DNA, Superhelical / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D008930:Mitochondria, Liver / Q000201:enzymology*; D010957:Plasmids; D051381:Rats; D013698:Templates, Genetic; D014158:Transcription, Genetic
D004272:DNA, Mitochondrial; D004274:DNA, Recombinant; D004278:DNA, Superhelical; D012321:DNA-Directed RNA Polymerases