[Determination of prostacyclin biosynthesis in biological specimens by the inhibition of thrombocyte aggregation].
The method of platelet aggregation inhibition was used for determination of prostacycline-like activity (PGI2) in the process of incubation of biological samples with arachidonic acid. The choice of optimal conditions to carry out the experiment allowed the authors to estimate the level of PGI2 formation in pieces of cardiac tissue (left and right ventricle, left and right auricles), kidneys (internal and external layers), thoracic aorta. Application of the method to investigate the selective effect on PGI2 biosynthesis in vitro will promote the development of new approaches to prevention and treatment of some cardiovascular diseases.
D000818:Animals; D001011:Aorta / Q000378:metabolism; D001095:Arachidonic Acids / Q000494:pharmacology; D011464:Epoprostenol / Q000032:analysis* / Q000096:biosynthesis; D006325:Heart Atria / Q000378:metabolism; D006352:Heart Ventricles / Q000378:metabolism; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism; D008297:Male; D008722:Methods; D010974:Platelet Aggregation / Q000187:drug effects*; D011453:Prostaglandins / Q000032:analysis*; D011817:Rabbits
D001095:Arachidonic Acids; D011453:Prostaglandins; D011464:Epoprostenol
Opredelenie biosinteza prostatsiklina v biologicheskikh obraztsakh metodom ingibirovaniia agregatsii trombotsitov.