Sequences of four tRNA genes from Caenorhabditis elegans and the expression of C. elegans tRNALeu (anticodon IAG) in Xenopus oocytes.
Four tRNA genes have been identified in cloned segments of Caenorhabditis elegans DNA by tRNA hybridisation and expression after injection into Xenopus laevis oocyte nuclei. From DNA sequencing these are (with DNA anticodon sequences) tRNAAsp (GTC), tRNALeu (AAG), tRNALys (CTT) and tRNAPro (TGG). Their flanking DNA sequences are compared. Two identical tRNALys (CTT) genes from different regions of the genome have quite unrelated 5' flanking sequences. The tRNA synthesised in Xenopus oocytes after injection of the tRNALeu cloned DNA has the modified anticodon IAG. The tRNALeu gene precursor transcript from injected oocytes has short 5' and 3' additional sequences and lacks certain of those modified bases found in the processed tRNA.
D000818:Animals; D000926:Anticodon / Q000235:genetics; D001483:Base Sequence; D002107:Caenorhabditis / Q000235:genetics*; D003001:Cloning, Molecular; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D005260:Female; D005796:Genes; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics*; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics*; D014158:Transcription, Genetic; D014981:Xenopus
D000926:Anticodon; D012346:RNA, Transfer, Amino Acyl; D012343:RNA, Transfer; D012321:DNA-Directed RNA Polymerases