Characterization of the viral RNA species of prototype dengue viruses.
Dengue virus isolates have been grown in the Igarashi clone C6/36 Aedes albopictus cells in amounts suitable for biochemical studies. Oligonucleotide fingerprint analyses have been used to characterize the virion 40 S RNA species of the four prototype dengue (DEN) serotype viruses: DEN 1, Hawaiian strain; DEN 2, New Guinea C strain (both insect and mouse brain passaged derivatives); DEN 3, H-87 strain; and DEN 4, H-241 strain. Comparisons of the largest oligonucleotides derived by ribonuclease T1 digestion of these 40 S DEN virion RNA species indicate that ther are few, if any, large oligonucleotides that are homologous between any two fo the four dengue prototype strains. Preliminary analyses of the 40 S RNA 5' terminal sequence of DEN 3 virion RNA indicate that it has the composition of m7GpppAmpXp.
D000330:Aedes; D000818:Animals; D003716:Dengue Virus / Q000032:analysis*; D000882:Haplorhini; D006801:Humans; D008253:Macaca mulatta; D009841:Oligonucleotides / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D013045:Species Specificity
D009841:Oligonucleotides; D012367:RNA, Viral