Growth studies on Mycobacterium BCG: establishment of growth curves and measurement of the oxygen tension of the growth medium.
Mycobacterium BCG grew exponentially in shallow, static volumes of culture medium for approximately 10 days; the oxygen tension of the medium at all stages of growth was 100% saturation. Higher yields were obtained from Dubos than from glycerol-free medium. In static cultures, the oxygen tension of the culture and consequently the growth rate of BCG was dependent on the depth of the medium; active growth ceased at an oxygen tension of less than 40% saturation. BCG grew actively in a cell sediment, while cells growing in suspension made a negligible contribution to the yield.
D003470:Culture Media; D009163:Mycobacterium bovis / Q000254:growth & development*; D010100:Oxygen / Q000032:analysis*
D003470:Culture Media; D010100:Oxygen