Failure to recover alpha-hemolytic streptococci or malignancy-associated microorganisms from patients with kidney disease and from healthy humans.
We duplicated blood culture techniques and media used by workers reporting alpha-hemolytic streptococci from nephropathy patients as well as from healthy persons. We studied 33 kidney patients presenting 12 diagnosed kidney diseases. The study was expanded to duplicate the experimental blood culture techniques of workers who reported Bacillus licheniformis and Cryptocides tumefaciens from patients with malignancies and, to a lesser degree, from healthy people. Seven culture media and 21 conditions of growth were used. There were no streptococcal isolates, and the few random isolates of other bacteria indicated that they were contaminants. Thus, our study did not corroborate the previous reports.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001431:Bacteriological Techniques; D003470:Culture Media; D005260:Female; D006801:Humans; D007674:Kidney Diseases / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000382:microbiology*; D013291:Streptococcus / Q000302:isolation & purification*; D013997:Time Factors
D003470:Culture Media