Improved double immunoenzyme labeling using alkaline phosphatase and horseradish peroxidase.
Alkaline phosphatase and peroxidase complexes were applied to tissue sections simultaneously to label two antigens. An azo-coupling method was used to demonstrate the alkaline phosphatase activity. The final result gave clear contrast between the two antigens and allowed the use of haematoxylin as the nuclear counterstain.
D000469:Alkaline Phosphatase / Q000032:analysis; D006416:Hematoxylin; D006735:Horseradish Peroxidase / Q000032:analysis; D007124:Immunoenzyme Techniques; D008722:Methods
D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase; D006416:Hematoxylin