Immunohistochemical demonstration of acid alpha-glucosidase in fetal rat hepatocytes and glycogenosomes of newborn rat hepatocytes.
The electron microscopic distribution of acid alpha-glucosidase in fetal and newborn rat hepatocytes was studied by a peroxidase labeled method. In fetal and newborn rat hepatocytes, acid alpha-glucosidase was found abundantly. The reaction products had a small granular appearance in each hepatic area under the light microscope. By electron microscopy, the enzyme was found to be located in the lysosomes, especially on the lysosome membranes, and sometimes within their contents. Glycogenosomes appearing in the hepatocytes obtained from newborn rats at 6 hours after birth showed a positive immunohistochemical reaction on their membranes and also within their contents. Furthermore, positive reactions were apparent on the Golgi apparatus in newborn rat hepatocytes. These results indicated that the glycogenosomes appearing in the early neonatal rat hepatocytes did not depend on a deficiency of acid alpha-glucosidase.
D000818:Animals; D000831:Animals, Newborn; D005959:Glucosidases / Q000032:analysis*; D006056:Golgi Apparatus / Q000201:enzymology; D006651:Histocytochemistry; D008099:Liver / Q000196:embryology / Q000201:enzymology* / Q000648:ultrastructure; D008247:Lysosomes / Q000648:ultrastructure; D008854:Microscopy, Electron; D051381:Rats; D000520:alpha-Glucosidases / Q000032:analysis*
D005959:Glucosidases; D000520:alpha-Glucosidases