Preparation of a partially desialylated human chorionic gonadotrophin (hCG) and its use for induction of ovulation after ovarian stimulation with human menopausal gonadotrophin.
A method for the large scale preparation of partially desialylated human chorionic gonadotrophin suitable for human use is reported. To obtain the desired grade of desialylation and to avoid the presence of the enzyme in the modified hormone, neuraminidase coupled to Sepharose 4B was used. The preparation showed to be active in vitro (OAAD and SVW tests) and its half-life was found to be 13 min in the rat and 75 min in human beings. This desialo hCG proved to be effective in inducing ovulation in amenorrhoeic women. Among 39 induced cycles 31 ovulations and 5 pregnancies occurred.
D000328:Adult; D000568:Amenorrhea / Q000188:drug therapy*; D000818:Animals; D001212:Asialoglycoproteins; D006063:Chorionic Gonadotropin / Q000302:isolation & purification / Q000494:pharmacology*; D004958:Estradiol / Q000097:blood; D005260:Female; D006801:Humans; D008596:Menotropins / Q000494:pharmacology*; D009439:Neuraminidase; D010053:Ovary / Q000187:drug effects*; D010060:Ovulation / Q000187:drug effects*; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D051381:Rats; D012685:Sepharose; D012794:Sialic Acids / Q000032:analysis; D014462:Ultrafiltration
D001212:Asialoglycoproteins; D006063:Chorionic Gonadotropin; D012794:Sialic Acids; C019263:asialo-human chorionic gonadotropin; D011374:Progesterone; D004958:Estradiol; D008596:Menotropins; D012685:Sepharose; D009439:Neuraminidase