Renin synthesis and gene cloning.
1. The present study describes the biosynthesis and gene cloning of mouse submandibular gland renin. 2. After translation of mRNA coding for renin a Mr 46 000 protein is produced (preprorenin). However, rapid removal of the 'pre' segment from the nascent chain would account for the fact that in pulse-chase and continuous labelling experiments the largest renin-immunoreactive protein seen was of Mr 44 500, pI 6.4 (determined by 2-dimensional gel electrophoresis). This 'prorenin' was rapidly converted to a Mr 40 000, pI 6.2 species and then more slowly to forms of Mr 35 500, pI 5.6 and Mr 34 000, pI 5.4, which correspond in Mr and pI to renin isolated in pure form from mouse submandibular glands. 3. Colonies of the bacterium Escherichia coli strain RRI, containing plasmid pBR322 into which mouse submandibular gland cDNA had been inserted at the PstI site, were screened with affinity-purified anti-renin. Of 500 colonies, two were found that reacted positively with the antibody. These contained protein that could be immunoprecipitated with anti-renin and cDNA which was capable of inter-colony hybridization and which had a HinfI restriction site in each case.
D000818:Animals; D001483:Base Sequence; D004274:DNA, Recombinant; D008297:Male; D051379:Mice; D008970:Molecular Weight; D010957:Plasmids; D012083:Renin / Q000096:biosynthesis* / Q000235:genetics
D004274:DNA, Recombinant; D012083:Renin