Isolation and re-association of the subunits from the pro-(carboxypeptidase A)-pro-(proteinase E) binary complex from pgi pancreas.
The component subunits of the pro-(carboxypeptidase A)-pro-(proteinase E) binary complex from pig pancreas were separated with a high recovery (80-95%) of their original potential activity. The isolated subunits and the reconstituted complex have properties similar to those of the corresponding natural species. The tryptic activation course of the pro-(carboxypeptidase A) subunit is substantially modified when bound to pro-(proteinase E), whereas the activation of pro-(proteinase E) is not dependent on this association.
D000818:Animals; D002268:Carboxypeptidases / Q000302:isolation & purification / Q000378:metabolism*; D043422:Carboxypeptidases A; D002846:Chromatography, Affinity; D010450:Endopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D004792:Enzyme Precursors / Q000302:isolation & purification / Q000378:metabolism*; D046911:Macromolecular Substances; D009097:Multienzyme Complexes / Q000302:isolation & purification / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology*; D013552:Swine; D014357:Trypsin / Q000494:pharmacology
D004792:Enzyme Precursors; D046911:Macromolecular Substances; D009097:Multienzyme Complexes; D002268:Carboxypeptidases; D010450:Endopeptidases; C035786:pro-proteinase E; D043422:Carboxypeptidases A; D014357:Trypsin