[Cellular regulation of the expression of i and I antigens during the in vitro differentiation of BFU-E].
Burst forming units (BFU-E) from the blood of eight normal adults were grown in methylcellulose culture. Follow up of i and I antigens detected by an indirect immunofluorescence technique from the 9th to the 14th day of culture revealed that i antigen expression diminished while maturation proceeded. On the contrary 1 antigen expression increased meanwhile. Nevertheless, in addition to this maturation process, expression of i antigen must be determined by a regulatory mechanism operating during erythroid stem cell differentiation since a large proportion of immature erythroblasts at the 10th day of culture do not express i antigen.
D000328:Adult; D000941:Antigens / Q000235:genetics*; D002454:Cell Differentiation; D002478:Cells, Cultured; D004900:Erythroblasts; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000166:cytology / Q000276:immunology*; D006801:Humans; D010641:Phenotype; D013997:Time Factors
D000941:Antigens
Régulation cellulaire de l'expression des antigènes i et I au cours de la différenciation in vitro des BFU-E.