Effect of androgen mediated by the estrogen receptor of fish liver:  vitellogenin accumulation.
We have investigated the action of high doses of androgens in Gobius niger L., a marine teleostean fish, by characterizing specific steroid receptors in liver and by assaying the plasma vitellogenin concentration under different hormonal treatments. Estrogen and androgen receptors were characterized in the liver nuclear extracts according to their binding specificity. The maximum binding capacity was 25 fmoles/mg protein for the estrogen and androgen receptors. In vivo, high doses of DHT()increased the concentration of plasmatic vitellogenin as assayed by immunodiffusion while low doses were inefficient. In spite of a similar number of estrogen and androgen nuclear receptor sites (25 fmoles/mg protein), DHT was at least 70 fold less active than E2 on yolk protein and vitellogenin induction both in male and female Gobius niger. In addition, the antiestrogen tamoxifen, which was inactive by itself, inhibited the E2 and the DHT induced accumulation of vitellogenin. Progesterone (2 mg/fish) was also totally inactive in inducing vitellogenin. We conclude that the induction of vitellogenin by DHT is mediated by the estrogen receptor rather than by the androgen receptor. In addition to the estradiol induced protein in rat uterus and to other estrogenic responses obtained by androgens in mammary cancer, fish vitellogenin is another estrogen regulated protein which can be induced by high doses of androgens.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism / Q000494:pharmacology*; D004530:Egg Yolk; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005399:Fishes / Q000378:metabolism*; D005779:Immunodiffusion; D008074:Lipoproteins / Q000097:blood*; D008099:Liver / Q000378:metabolism*; D008297:Male; D011944:Receptors, Androgen / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D013629:Tamoxifen / Q000494:pharmacology; D014819:Vitellogenins / Q000097:blood*
D008074:Lipoproteins; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D011987:Receptors, Steroid; D014819:Vitellogenins; D013196:Dihydrotestosterone; D013629:Tamoxifen; D004958:Estradiol