[Temperature-sensitive mutant of bacteriophage D3 and its use for the purpose of demonstrating the location of prophage in Pseudomonas aeruginosa cells].
For the first time a thermoinducible mutant, known as D3ct, has been obtained from Pseudomonas aeruginosa phage and used for lysogenizing culture PAO2604 Met--Ilv--. After a temperature shock phage D3ctwas induced from lysogenic strain PAO2604 (D3ct), and its development went along the lytic line. From the population of PAO2604 (D3ct) cells only 0.07% survived; in these cells the faulty excision of the prophage probably occurred at a high temperature. After thermoinduction 2.8% of the colonies formed by the survivors were auxotrophic in leucine. Such frequency of the appearance of the additional nutritional requirement for leucine suggests that the prophage was incorporated into the chromosome adjacent to some gene responsible for biosynthesis of leucine. In the process of faulty excision phage D3 retained a part of the host chromosome.
D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification; D007930:Leucine / Q000235:genetics; D008242:Lysogeny; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D013696:Temperature; D014775:Virus Activation
D007930:Leucine
Temperaturochuvstvitel'nyĭ mutant bakteriofaga D3 i ispol'zovanie ego s tsel'iu vyiasneniia lokalizatsii profaga v kletkakh Pseudomonas aeruginosa.