Propagating Bacillus subtilis spores in a liquid medium for the Guthrie bioassay.
Eagle growth medium was used successfully for the production of spores of Bacillus subtilis for the Guthrie bioassay screening tests for genetic metabolic disorders.
D001412:Bacillus subtilis / Q000502:physiology*; D001681:Biological Assay; D003470:Culture Media; D013171:Spores, Bacterial / Q000254:growth & development
D003470:Culture Media