Neurohypophysial-hormone-responsive cell line derived from a dimethylbenzanthracene-induced rat mammary tumour.
WRK-1, a cloned cell line derived from a rat mammary tumour, responds to physiological concentrations of vasopressin and pharmacological concentrations of oxytocin with increased incorporation of [14C]acetate into lipids and increased protein accumulation. The presence of pharmacological concentrations of insulin, which itself is active on the WRK-1 cells, further enhances the effects of the neurohypophysial hormones. Unlike the action of vasopressin on other responsive tissues, the stimulation of acetate incorporation by WRK-1 cells is not observed until 24 h after the addition of the hormone. The lipids synthesized in response to the hormones are predominantly polar lipids, rather than the triclyclycerold characteristic of the differentiated mammary gland. [1-Deaminocysteine, 8-D-arginine] vasopressin, a vasopressin analogue that lacks pressor activity, has no effect on WRK-1 cells.
D015127:9,10-Dimethyl-1,2-benzanthracene; D000085:Acetates / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D005260:Female; D007328:Insulin / Q000494:pharmacology; D050356:Lipid Metabolism; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced; D010121:Oxytocin / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D013268:Stimulation, Chemical; D014667:Vasopressins / Q000494:pharmacology*
D000085:Acetates; D007328:Insulin; D011506:Proteins; D014667:Vasopressins; D010121:Oxytocin; D015127:9,10-Dimethyl-1,2-benzanthracene