[Biosynthetic incorporation of fluorescently labelled fatty acids in Escherichia coli].
Escherichia coli cells were cultivated in a medium containing 1-pyrene butanoic acid, a fluorescent probe. Total lipids were extracted from the cells, and the extract was separated by thin-layer chromatography. The fluorescent fractions were examined using spectrofluorimetry. The starting 1-pyrene butanoic acid was shown to be biosynthetically incorporated into the bacterial lipid. Four fluorescent fractions appeared as a result; the fractions were derivatives of this compound modified in the chromophore and the fatty acid chain. The results indicate that the formation of 1-pyrene butanoic acid fluorescent metabolites can be used for studying the oxidation-reduction systems of the bacterium.
D002462:Cell Membrane / Q000378:metabolism; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000378:metabolism*; D005227:Fatty Acids / Q000096:biosynthesis*; D005456:Fluorescent Dyes / Q000378:metabolism; D011721:Pyrenes / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D005227:Fatty Acids; D005456:Fluorescent Dyes; D011721:Pyrenes; C002218:1-pyrenebutyrate
Biosinteticheskoe vkliuchenie fluorestsentno mechenykh zhirnykh kislot v Escherichia coli.