[Effect of plasmid pKM101 on the mutagenic action of dioxidine in Escherichia coli K-12].
To define the types of base pair substitutions induced by the antibacterial agent dioxydine, the genotypes of Trp+ revertants of the trpA58 and trpA78 missense mutations were studied. Dioxydine was shown to induce transitions and transversions in the plasmidless strains, AT leads to GC transitions and, probably, GC leads to TA transversions exceeding other types of base changes. The strains harbouring pKM101 plasmid had the higher rate of mutations induced by dioxydine due to the increase in frequencies of AT leads to GC transversions and transitions of GC pairs. Thus, the mutator activity mediated by pKM101 results in a significant change in distribution of predominant types of dioxydine-induced base pair substitutions.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence / Q000187:drug effects; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D005838:Genotype; D009153:Mutagens / Q000494:pharmacology*; D010957:Plasmids / Q000187:drug effects*; D011810:Quinoxalines / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D009153:Mutagens; D011810:Quinoxalines; C001077:dioxidine
Vliianie plazmidy pKM101 na mutagennoe deĭstvie dioksidina u Escherichia coli K-12.