Characterization of DNA polymerases in mature sperm of the sea urchin.
In the extract of mature sperm of the sea urchin, Hemicentrotus pulcherrimus, two species of DNA polymerase (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7.) activity were detected. One of them was eluted from a hydroxyapatite column at 0.08 M phosphate buffer and was insensitive to both N-ethylmaleimide and aphidicolin. The sedimentation coefficient was 3.3 S and the isoelectric point was pH 8.6. This activity corresponds to the DNA polymerase-beta activity. The other was eluted from the hydroxyapatite column at 0.14 M phosphate buffer and was inhibited by N-ethylmaleimide but not by aphidicolin. The sedimentation coefficient was 3.3 S and 6.0 S and the isoelectric point was pH 4.8. The template primer preference of this enzyme was coincident with DNA polymerase-gamma. The DNA polymerase-alpha activity was not detected in the extract of mature sperm.
D000818:Animals; D016590:Aphidicolin; D002499:Centrifugation, Density Gradient; D004256:DNA Polymerase I / Q000302:isolation & purification / Q000378:metabolism; D004258:DNA Polymerase III / Q000302:isolation & purification / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification*; D004224:Diterpenes / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D008297:Male; D012617:Sea Urchins; D013094:Spermatozoa / Q000201:enzymology*; D013698:Templates, Genetic
D004224:Diterpenes; D016590:Aphidicolin; D004256:DNA Polymerase I; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase; D005033:Ethylmaleimide