Degradation of amyloid proteins with protease I from Aspergillus oryzae. In vivo increase in SAA clearance rate after enzyme infusion.
The effect of the thrombolytic enzyme protease I from Aspergillus oryzae (brinase) on the amyloid protein AA was investigated. The effect of the enzyme on purified, low molecular weight protein AA was very high. Protein AA in intact amyloid fibrils suspended in neutral buffer was also degraded by the enzyme, although at a much lower rate. Amyloid fibrils in tissue sections could also be attacked and removed, leaving the tissue structure fairly intact. The in vivo effect of brinase on protein SAA was demonstrated in rabbits by an increase in the clearance rate of SAA after enzyme infusion.
D000682:Amyloid / Q000378:metabolism*; D000686:Amyloidosis / Q000378:metabolism; D000818:Animals; D001954:Brinolase / Q000008:administration & dosage / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007263:Infusions, Parenteral; D008099:Liver / Q000032:analysis / Q000378:metabolism; D010447:Peptide Hydrolases / Q000494:pharmacology*; D011817:Rabbits; D000685:Serum Amyloid A Protein / Q000378:metabolism*
D000682:Amyloid; D000685:Serum Amyloid A Protein; D010447:Peptide Hydrolases; D001954:Brinolase