[Formation of donor strains of enteropathogenic Escherichia, serogroups O112ac, O129, O143, and Sh. boydii 8 using R-plasmids].
The possibility of the formation of enteropathogenic Escherichia and Shigella donor strains by means of R-plasmids RP4 and RPI-2 with thermosensitive replication was studied. The donor strains of E. coli, serogroups O112ac, O129, O143, and Sh. boydii 8 were thus obtained; these strains had the above-mentioned plasmids integrated into their chromosome, and in the process of conjugation they transferred plasmid and chromosomal genes to E. coli, K12 and O129, with the effectiveness 10(5)--10(8). Such donor strains may probably be used for studying the mechanisms of the formation of new enteropathogenic Escherichia and Shigella biovars and for their genetic analysis.
D002876:Chromosomes, Bacterial / Q000648:ultrastructure; D003433:Crosses, Genetic; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D005838:Genotype; D011550:Pseudomonas aeruginosa / Q000235:genetics; D011815:R Factors; D012703:Serotyping; D012760:Shigella / Q000235:genetics*; D012761:Shigella boydii / Q000235:genetics*
Formirovanie donorskikh shtammov énteropatogennykh ésherikhiĭ serogrupp O112ac, O129, O143 i Sh. boydii 8 s pomoshch'iu R-plazmid.