Large-scale purification of IgM from human sera. Comparison of three optimized procedures utilizing protein A chromatography.
for the preparation of gram amounts of IgM from human sera sedimentation at 100,000 g or treatment with ZnSO4 of the redissolved "euglobulin"-precipitate was compared to direct precipitation from the clarified serum by boric acid. Three alternative large scale purification procedures were developed, leading to an IgM-sample characterized as pure by various criteria. Inclusion of protein A chromatography proved to enhance the yield very considerably.
D001888:Boric Acids; D011232:Chemical Precipitation; D002850:Chromatography, Gel / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D007075:Immunoglobulin M / Q000302:isolation & purification*; D013205:Staphylococcal Protein A; D014461:Ultracentrifugation; D008258:Waldenstrom Macroglobulinemia / Q000276:immunology; D015032:Zinc
D001888:Boric Acids; D007075:Immunoglobulin M; D013205:Staphylococcal Protein A; D015032:Zinc