Red blood cell adhesion. I. Determination of the ionic conditions for adhesion to an oil-water interface.
We have examined the adhesion of glutaraldehyde-treated human red blood cells to a clean liquid hydrocarbon interface as a function of sodium chloride concentration. Cells adhere reversibly to the interface over a wide range of concentrations but fail to do so below about 0.1 mM. Adherent cells do not alter the tension of the oil/water interface. These results show that cells can adhere by physical forces in special situations without biochemical interactions. The data provide a basis for calculating the size of the physical forces.
D000473:Alkanes; D002448:Cell Adhesion; D004586:Electrophoresis; D004912:Erythrocytes / Q000502:physiology*; D005976:Glutaral; D006801:Humans; D066298:In Vitro Techniques; D009994:Osmolar Concentration; D012965:Sodium Chloride; D013500:Surface Tension
D000473:Alkanes; D012965:Sodium Chloride; D005976:Glutaral