[Comparative study of the immunochemical properties of the 5th lactate dehydrogenase isoenzyme from rat skeletal muscles and liver. I. The isolation and characteristics of LDH-5 preparations from skeletal muscles].
Individual LDH-5 preparations from rat skeletal muscles have been obtained by differential fractionation with (NH4)2SO4 and precisional ion exchange chromatography. According to the data of ultracentrifugation, electrophoresis in acrilamide gel, enzyme-electrophoresis and agar precipitation reaction with antimuscle serum, these preparations have no admixtures of other isoenzymes of foreign proteins. Examination of antigenic properties of these LDH-5 preparations may suggest the availability of common antigenic determinates in the liver extract and in the LDH-5 preparation.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D005779:Immunodiffusion; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000276:immunology* / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology*; D051381:Rats; D014461:Ultracentrifugation
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase
Sravnitel'noe izuchenie immunokhimicheskikh svoĭstv piatogo izozima laktatdegidrogenazy iz skeletnykh myshts i pecheni krys. I. Poluchenie i kharakteristika preparatov LDG-5 iz skeletnykh myshts.