Procedure for titration of interleukin-2 based on direct observation of factor-dependent growth of cells.
A semi-quantitative procedure for titration of interleukin-2 (IL-2) without the use of radioactive labeling of IL-2 target cells was developed. The procedure is based on the observation that those cells cultivated in round-bottom wells formed visible cultures and that the dimension of these cultures decreased at higher dilutions of IL-2 in the medium. The end-point of the titration can be easily established by observing the cultures with a concave mirror placed under the titration plate. The procedure has been used for the determination of IL-2 during its purification and characterization.
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D003584:Cytological Techniques; D007376:Interleukin-2 / Q000032:analysis*; D008213:Lymphocyte Activation; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000187:drug effects*; D013950:Thymus Gland / Q000166:cytology
D003470:Culture Media; D007376:Interleukin-2; D003208:Concanavalin A