Role of I-region gene products in macrophage induction of an antibody response. II. Restriction at the level of T cell in recognition of I-J-subregion macrophage determinants.
The effect of specific anti-I-J reagents on macrophage-T cell interactions was studied in an in vitro antibody response to burro erythrocytes. Macrophages were prepared from the spleens of F1 hybrid mice whose parental strains differed at the I-J subregion. Two F1 hybrids were used for these experiments, [B10.A(3R) X B10.A(5R)]F1 and [B10.S(9R) X B10.HTT]F1. F1 macrophages responded equally well with F1 T-B cells or with T-B cells of either parental strain. When F1 macrophages were pretreated with anti-I-J serum (without complement) specific for one parental haplotype, they were only able to cooperate with T helper (TH) cells of the unblocked haplotype and with F1 TH cells. Identical results were obtained with (Jb X Jk)F1 and (Js X Jk)F1 mice. The results indicate that TH cells possess genetically restricted receptors for macrophage I-J-subregion gene products and that the interaction between this receptor and the macrophage I-J-subregion determinants is essential for the initiation of a primary in vitro antibody response to an erythrocyte antigen.
D000818:Animals; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface; D005802:Genes, MHC Class II; D007518:Isoantibodies; D007519:Isoantigens; D008211:Lymphocyte Cooperation; D008264:Macrophages / Q000276:immunology*; D008285:Major Histocompatibility Complex; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D007518:Isoantibodies; D007519:Isoantigens