Decreased precipitation of factor VIII related antigen with concanavalin A in patients with a variant of von Willebrand's disease and disseminated intravascular coagulation.
Precipitation pattern of factor VIII related antigen (VIIIR:AG) with concanavalin A (Con A), a glycoprotein binding lectin, was investigated in normal subjects and patients with a variant of von Willebrand's disease (VWD) and disseminated intravascular coagulation (DIC). Decreased precipitation with Con A was demonstrated in patients with a variant of VWD in which VIIIR:AG showed an increase in electrophoretic mobility on crossed immunoelectrophoresis (CIE). Similar findings were obtained in patients with DIC which revealed normal electrophoretic mobility of VIIIR:AG. This procedure was assumed to be useful for the detection of a qualitative abnormality of the factor VIII protein.
D000941:Antigens / Q000032:analysis*; D003208:Concanavalin A; D004211:Disseminated Intravascular Coagulation / Q000276:immunology*; D005169:Factor VIII / Q000276:immunology*; D006801:Humans; D011233:Precipitin Tests; D014842:von Willebrand Diseases / Q000276:immunology*
D000941:Antigens; D003208:Concanavalin A; D005169:Factor VIII