Improved conversion of methanol to single-cell protein by Methylophilus methylotrophus.
The glutamate dehydrogenase gene of Escherichia coli has been cloned into broad host-range plasmids and can complement glutamate synthase mutants of Methylophilus methylotrophus. Assimilation of ammonia via glutamate dehydrogenase is more energy-efficient than via glutamate synthase, thus the recombinant organism converts more growth substrate, methanol, into cellular carbon.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis*; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000201:enzymology; D005818:Genetic Engineering / Q000379:methods; D005969:Glutamate Dehydrogenase / Q000235:genetics*; D005970:Glutamate Synthase / Q000378:metabolism; D000432:Methanol / Q000378:metabolism; D008772:Methylococcaceae / Q000201:enzymology / Q000378:metabolism*; D010957:Plasmids
D001426:Bacterial Proteins; D004274:DNA, Recombinant; D000641:Ammonia; D000255:Adenosine Triphosphate; D005970:Glutamate Synthase; D005969:Glutamate Dehydrogenase; D000432:Methanol