A grinding method for producing sections of large areas of plastic embedded tissues.
We have developed a method utilizing relatively thick ground sections of plastic embedded tissue which affords the resolution obtained with 0.5 micrometers cut sections. The sections, which are permanently affixed to plastic microscope slides, are much larger in area than ultramicrotome sections. Additional advantages are: sections can be destained and restained and selected areas can be examined with various forms of electron microscopy. Autoradiographic studies are also possible. Although the method has a broader application, it is particularly useful in examining the interface between hard and soft tissues.
D000818:Animals; D001345:Autoradiography; D006652:Histological Techniques; D007180:Incisor / Q000166:cytology; D008853:Microscopy / Q000379:methods*; D051381:Rats; D012469:Salivary Glands / Q000166:cytology; D013048:Specimen Handling / Q000379:methods