[Characteristics of the NAD-dependent alpha-glycerolphosphate dehydrogenase isoenzymes from vertebrate and human muscles according to polyacrylamide gel electrophoretic and isoelectric focusing data].
Electrophoresis and isoelectric focusing in polyacrylamide gel were used to demonstrate the presence of 5 molecular forms of alpha-glycerophosphate dehydrogenase (E. C. I. I. I. 8, alpha-GDH) in the cytoplasm of some vertebrata skeletal muscles. Assay of alpha-GDH isozyme distribution in the muscles marked by the prevalent type of oxidation disclosed the higher activity of the cathodic forms--alpha-GDH4 and alpha-GDH5--in the white muscle (m. quadratus lumborum) as compared with the red one (m. soleus). It is suggested that the study of alpha-GDH molecular form distribution may serve as an objective criterion for changes in the ratio of the aerobic and anaerobic processes in animal tissues under different states of animal body.
D000328:Adult; D000818:Animals; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005993:Glycerolphosphate Dehydrogenase / Q000032:analysis*; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis*; D009132:Muscles / Q000201:enzymology*; D009243:NAD / Q000378:metabolism*; D011817:Rabbits; D051381:Rats
D007527:Isoenzymes; D009243:NAD; D005993:Glycerolphosphate Dehydrogenase
Kharakteristika izofermentov NAD-zavisimoĭ al'fa-glitserofosfatdegidrogenazy iz myshts riada pozvonochnykh i cheloveka po dannym élektroforeza i izoélektricheskogo fokusirovaniia v poliakrilamidnon gele.