An improved method for HBcAg demonstration in paraffin-embedded liver tissue.
Factors influencing the preservation of HBcAg in formalin-fixed, paraffin-embedded liver tissue have been analysed. Fixation time, choice of the embedding medium, deparaffinization and selection of anti-HBc proved to be critical variables. For optimal results, fixation in 4% buffered formalin for no longer than 24-48 h, the use of wax with a low melting point, a heating step prior to deparaffinization, and a search for anti-HBc specifically reactive for paraffinated HBcAg are recommended.
D005404:Fixatives; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde; D005629:Frozen Sections; D006512:Hepatitis B Core Antigens / Q000032:analysis*; D006652:Histological Techniques; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000032:analysis*; D010232:Paraffin
D005404:Fixatives; D006512:Hepatitis B Core Antigens; D005557:Formaldehyde; D010232:Paraffin