Induction and regulation of immunoglobulin expression in a murine pre-B cell line, 70Z/3. I. Cell cycle-associated induction of sIgM expression and kappa-chain synthesis in 70Z/3 cells by LPS stimulation.
Stimulation of a murine pre-B cell line, 70Z/3, with LPS induced sIgM expression and an increase of L kappa-chain mRNA within 12 hr. In synchronized 70Z/3 cells, the cell division cycle was 10 to 11 hr and LSP-stimulation did not affect the cell division cycle. LPS-signals given at G1/S boundary induced sIgM expression in the G2 to M-phase. On the other hand, LPS-signals given after M-phase did not induce sIgM expression. Inhibition of cell division with demecorcin did not affect sIgM expression in the M-phase. Induction of intracellular kappa-chain synthesis was also observed in the G2 to M-phase when the LPS-stimulation was provided at G1/S boundary, but LPS-signals given after the M-phase did not induce de novo synthesis of kappa-chain. These results showed that LPS-induced sIgM expression and synthesis of kappa-chain were cell cycle-related events and sIgM expression was associated wih de novo synthesis of kappa-chain.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002453:Cell Cycle; D002460:Cell Line; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis*; D007075:Immunoglobulin M; D007145:Immunoglobulin kappa-Chains / Q000096:biosynthesis*; D007148:Immunoglobulin mu-Chains; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D011947:Receptors, Antigen, B-Cell
D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D007145:Immunoglobulin kappa-Chains; D007148:Immunoglobulin mu-Chains; D008070:Lipopolysaccharides; D011947:Receptors, Antigen, B-Cell