[Morphological method of differentiating T- and B-lymphocytes by detecting nonspecific alpha-naphthylacetate esterase].
A modified Gomori method for detecting nonspecific alpha-naphthylesterase is proposed as a morphological means for differentiation of T- and B-lymphocytes. The final product of the reaction was shown to be detected in the cytoplasm of lymphoid cells composing T-zones of organs of the immune system and also in the lymphocytes forming E-rosettes but not in the cells of B-zone. The method can be applied for morphological analysis of cellular immunity.
D000135:Acid Phosphatase / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001402:B-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D003584:Cytological Techniques; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D009283:Naphthol AS D Esterase / Q000032:analysis*; D011817:Rabbits; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology
D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase
Morfologicheskiĭ metod differentsirovki T- i B-limoftsitov putem vyiavleniia nespetsificheskoĭ al'fa-naftilatsetatésterazy.