Selective isolation and enumeration of low numbers of Staphylococcus aureus by a procedure that relies on elevated-temperature culturing.
A procedure for the selective detection of Staphylococcus aureus was elaborated, consisting of three parts: (i) enrichment in liquid Baird-Parker medium; (ii) isolation on Baird-Parker agar incubated at 43 degrees C, and (iii) confirmation by means of the test for thermostable nuclease. Elevated-temperature incubation of Baird-Parker medium did not make it inhibitory to cells stressed by heat treatment or osmotic shock.
D001431:Bacteriological Techniques; D003470:Culture Media; D003851:Deoxyribonucleases / Q000378:metabolism; D006358:Hot Temperature; D013211:Staphylococcus aureus / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media; D003851:Deoxyribonucleases