Hemolysis due to human antibody-dependent cell-mediated cytotoxicity. Lysis of erythrocytes treated with anti-D serum.
Antibody-dependent cell-mediated cytotoxicity against Rh+ red blood cells was detected in human anti-D sera. We used a rapid 4-h 51Cr-release assay to show that cytotoxic activity was proportional to serum concentration, incubation time and the attacking cell concentration. Attacking cells were obtained from normal human peripheral blood by Ficoll-Hypaque separation. Incubation of these lymphoid cells on a nylon column prior to the test depleted the number of phagocytic (latex-positive) cells in the effluent concomitantly with a drop in cytotoxic activity. Enrichment of the attacking cell population in mononuclear phagocytes by albumin gradient separation led to an increase in cytotoxicity. Granulocytes separated by Ficoll-Hypaque were not active in this system. Antibody activity was found in the 7S region following Sephadex G-200 fractionation of anti-D serum. Antibody activity was also studied after its elution from antiserum-coated red blood cells by diethyl ether. The eluate contained IgG and mediated cell-dependent lysis. Cell-free antiserum did not mediate lysis of coated red blood cells in the presence of complement.
D000920:Antibody-Dependent Cell Cytotoxicity; D003167:Complement Activation; D004306:Dose-Response Relationship, Immunologic; D004912:Erythrocytes / Q000276:immunology*; D006098:Granulocytes / Q000276:immunology; D006461:Hemolysis; D006801:Humans; D007074:Immunoglobulin G; D007518:Isoantibodies; D009000:Monocytes / Q000276:immunology; D012204:Rh-Hr Blood-Group System
D007074:Immunoglobulin G; D007518:Isoantibodies; D012204:Rh-Hr Blood-Group System