The effect of endogenous proteases on the spectrin binding proteins of human erythrocytes.
We have demonstrated that in human erythrocyte ghosts endogenous proteolytic activity is responsible for the digestion of the spectrin binding proteins (bands 2.1 to 2.6). The pH optimum, cofactor requirements and inhibitor sensitivity have been established. Our results indicate that proteolysis of bands 2.1 to 2.6 and the formation of 3', a fragment containing an active spectrin binding site, can occur through two enzymatic pathways: a cascade of consecutive proteolytic cleavages of the spectrin binding proteins inhibited by phenylmethylsulfonyl fluoride or a Ca2+-stimulated, phenylmethylsulfonyl fluoride-insensitive, EDTA-inhibited cleavage of band 2.1 to band 2.3, followed by digestion to band 3' by phenylmethylsulfonyl fluoride-inhibitable enzymes. These findings may provide the techniques necessary to prevent proteolysis of the spectrin binding proteins during purification and reconstitution experiments and provide insight into how they are formed in vivo.
D002118:Calcium / Q000494:pharmacology; D002352:Carrier Proteins / Q000097:blood*; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008565:Membrane Proteins / Q000097:blood* / Q000378:metabolism*; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000097:blood*; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D013049:Spectrin / Q000097:blood* / Q000378:metabolism*
D002352:Carrier Proteins; D008565:Membrane Proteins; C025957:spectrin-binding proteins; D013049:Spectrin; D010664:Phenylmethylsulfonyl Fluoride; D004492:Edetic Acid; D010447:Peptide Hydrolases; D002118:Calcium