A simple method for the isolation of phages from Listeria monocytogenes.
By application of a combined mitomycin-/heat treatment after freezing, 7 out of 29 Listeria monocytogenes strains which were found to be non-phage carriers by UV irradiation could release phages. Propagation of the obtained phages was promoted by storage at 4 degrees C. Apart from TNSA plates the application of chocolate plates appears to be necessary in order to study these phages.
D000818:Animals; D001435:Bacteriophages / Q000302:isolation & purification*; D002099:Cacao; D002417:Cattle; D003080:Cold Temperature; D003470:Culture Media; D005260:Female; D006358:Hot Temperature; D006801:Humans; D008089:Listeria monocytogenes; D008297:Male; D008828:Microbiological Techniques; D008937:Mitomycins; D014775:Virus Activation; D014776:Virus Cultivation
D003470:Culture Media; D008937:Mitomycins