[Comparative study of the circular dichroism of rabbit liver and muscle glycogen phosphorylases a and b].
Circular dichroism (CD) spectra of glycogen phosphorylase a and b from rabbit liver have been measured in the presence of various ligands in the near- and far-ultraviolet regions. Positive circular dichroism was detected in the absorption band of protein-bound pyridoxal phosphate (333 nm). The mean residue ellipticity of this dichroic band (35 deg cm2dmol-1) is of the same order for muscle and liver phosphorylase a and b and does not change upon binding of glucose-1-phosphate and AMP. Only glucose induces small changes in the ellipticity in this region. The CD spectra of muscle and liver phosphorylase a and b in the 250-300 nm region have at least five positive dichroic bands namely at 259, 264, 273, 281 and 288 nm and have strong resemblances for all these forms of the enzyme in spite of the fundamental differences in their properties. The binding of AMP and glucose to phosphorylase from both sources induces distinct perturbations in CD spectra; the changes are much larger for muscle and liver phosphorylase a than for phosphorylase b which indicates that conformational perturbations induced by binding of activator and inhibitor to the inactive form of phosphorylase are probably more local than for the active form. The CD spectra in far-ultraviolet region are similar for all forms of phosphorylase. The percent of alpha-helices calculated according to Chen is about 50; this value coincides very well with the value 51% received for muscle phosphorylase a by X-ray crystallographic analysis at 2.5 A resolution.
D000249:Adenosine Monophosphate; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D008099:Liver / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology*; D009928:Organ Specificity; D010762:Phosphorylase a; D010763:Phosphorylase b; D006005:Phosphorylases; D011487:Protein Conformation; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate
D012967:Sodium Dodecyl Sulfate; D000249:Adenosine Monophosphate; D010762:Phosphorylase a; D010763:Phosphorylase b; D006005:Phosphorylases
Sravnitel'noe issledovanie krugovogo dikhroizma glikogenfosforilaz a i b iz myshts i pecheni krolika.