A rapid photoelectric method for reading cell migration from agarose microdroplets.
A rapid photoelectric method for reading cell migration from agarose microdroplets is described. Practically instantaneous, the method eliminates drawing and planimetry and makes feasible the use of a wide range of antigen concentrations. The results obtained are similar to those obtained by planimetry, but the photoelectric method is more sensitive. Enhancement of migration as well as inhibition were significantly demonstrated by this method. Migration inhibition of immune mouse spleen cells was found to be bizonal, with 2 peaks, one at very low antigen concentrations (10(-3) microgram/ml ovalbumin) and one at 10 microgram/ml.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002464:Cell Migration Inhibition; D002465:Cell Movement; D007158:Immunologic Techniques; D008214:Lymphocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008813:Mice, Inbred ICR; D008853:Microscopy / Q000379:methods; D010047:Ovalbumin / Q000276:immunology; D011134:Polysaccharides / Q000494:pharmacology*; D012685:Sepharose / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology
D011134:Polysaccharides; D010047:Ovalbumin; D012685:Sepharose