The establishment of continuous macrophage cell lines from peripheal blood monocytes.
Peripheral blood monocytes were established in cultured from ten different animal species. Using a method of repeated medium changes, monolayers of cells developed from seven of these species. These cells had the following characteristics: they were glass and plastic adherent, phagocytic, possessed many vacuoles and pseudopodia, esterase-positive, exhibited complement and Fc receptors and could partake in antigen-specific immune induction of T cells. These characteristics classify the cells as macrophages. The monolayers could be serially passaged and used for virological and immunological assays.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D002460:Cell Line; D006041:Goats; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D010587:Phagocytosis; D011951:Receptors, Complement; D012756:Sheep; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology; D014779:Virus Replication; D014780:Viruses / Q000254:growth & development
D011951:Receptors, Complement