Effect of DNA length on the nucleosome low salt transition.
The effect of DNA length on the low salt unfolding transition of nucleosomes has been studied by the use of fluorescently labeled histones. Nucleosomes were formed by the reconstitution of bulk DNA fragments averaging 173 and 250 base pairs in length. These nucleosomes exhibited a conformational change in a transition centered at about 7 mM ionic strength, very different from that observed for the standard 145 bp nucleosomes (1-3mM). In addition, the conformational change of the 173 and 250 bp nucleosomes involves twice as many ions as that of the 145 bp nucleosomes.
D000818:Animals; D002645:Chickens; D004247:DNA / Q000378:metabolism*; D004492:Edetic Acid; D005456:Fluorescent Dyes; D006657:Histones / Q000378:metabolism; D007460:Iodoacetamide / Q000031:analogs & derivatives; D008968:Molecular Conformation; D009282:Naphthalenesulfonates; D009707:Nucleosomes / Q000378:metabolism*; D009994:Osmolar Concentration; D014325:Tromethamine
D005456:Fluorescent Dyes; D006657:Histones; D009282:Naphthalenesulfonates; D009707:Nucleosomes; D014325:Tromethamine; C006421:1,5-I-AEDANS; D004247:DNA; D004492:Edetic Acid; D007460:Iodoacetamide