Increased incorporation of 14C-palmitate into tissue lipids by isolated heart myocytes in endotoxic shock.
The incorporation of 14C-palmitate into various classes of tissue lipids by isolated adult dog heart myocytes was studied in an attempt to understand the pathophysiology of myocardial dysfunction during endotoxic shock. The results showed that the incorporation of 14C-palmitate into phospholipids was increased by 85.3% and 108.8% at 0.5 hours and two hours, respectively, following endotoxin (0.5 mg Escherichia coli lipopolysaccharide B per kg body weight) administration. Incorporation of radioactive palmitate into triglycerides was increased by 50.9% and 107.2% at 0.5 and two hours, respectively, postendotoxin. Incorporation of 14C-palmitate into diglycerides was stimulated by 51.9% and 64.5% at 0.5 and two hours, respectively, after endotoxin injection. The incorporation of 14C-palmitate into tissue-free fatty acids and unaltered at 0.5 hours but it was increased by 211.7% at two hours postendotoxin. These data demonstrated that myocardial membrane lipid profile was greatly altered by increased incorporation of 14C-palmitate into phospholipids and neutral lipids after endotoxin administration. An alteration in myocardial lipid profile, as reported in this study, may contribute to the development of myocardial dysfunction during shock.
D000214:Acyl Coenzyme A / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D004075:Diglycerides / Q000096:biosynthesis; D004285:Dogs; D004731:Endotoxins / Q000494:pharmacology; D004926:Escherichia coli; D005230:Fatty Acids, Nonesterified / Q000096:biosynthesis; D005260:Female; D066298:In Vitro Techniques; D008055:Lipids / Q000096:biosynthesis*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010168:Palmitates / Q000378:metabolism*; D010169:Palmitic Acids / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis; D012772:Shock, Septic / Q000378:metabolism*; D014280:Triglycerides / Q000096:biosynthesis
D000214:Acyl Coenzyme A; D002250:Carbon Radioisotopes; D004075:Diglycerides; D004731:Endotoxins; D005230:Fatty Acids, Nonesterified; D008055:Lipids; D010168:Palmitates; D010169:Palmitic Acids; D010743:Phospholipids; D014280:Triglycerides