Collagen, factor VIII antigen, and immunoglobulins in the human aqueous drainage channels.
Twenty-five trabeculectomy specimens from patients with primary open angle glaucoma and chronic angle closure glaucoma, and 11 age-matched controls were examined by immunofluorescence and immunoperoxidase techniques to determine the types of collagen, immunoglobulins, and the presence of factor VIII-related antigen in the human aqueous drainage channels. In the glaucoma cases and in controls, we demonstrated that the electron dense basement membrane-like material in the peripheral portion of the trabecular beams and in the juxtacanalicular meshwork, consists at least in part, of type IV collagen, a noncollagenous protein ("laminin") and fibronectin. Factor VIII-related antigen was demonstrated in conjunctival vessels of the control eyes. Schlemm's canal and the trabecular endothelial cells did not stain for factor VIII-related/antigen in any of the specimens examined. No deposits of IgA, IgM, IgG, and the C3 component of complement were detected in the aqueous drainage channels.
D000368:Aged; D000941:Antigens; D001082:Aqueous Humor / Q000276:immunology* / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism*; D003176:Complement C3 / Q000378:metabolism; D005169:Factor VIII / Q000276:immunology*; D005260:Female; D005901:Glaucoma / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D014129:Trabecular Meshwork / Q000276:immunology
D000941:Antigens; D003176:Complement C3; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D005169:Factor VIII; D003094:Collagen