Involvement of peritoneal macrophages and spleen stromal cells in X-irradiation-induced reticulum cell neoplasms in C57BL/6 mice.
Some correlation was observed between the occurrence of FA-positive PE-MO and spleen stromal cells (removed from X-irradiated RCN-bearing old-adult B6 mice) and the generation of RCN. No significant correlation was found between the viral content of lymphoid organs from the same mice and the occurrence of RCN. The main viral particle detected in lymphoid organs from radiation-induced RCN-bearing mice was the xenotropic virus. Ecotropic viruses were detected in a few spleens and Payer patches from such mice. These ecotropic viruses showed very poor lymphomagenic activity and required 400R X-ray as a cofactor. No dualtropic viruses were detected. However, inoculation of ecotropic (SFA2) helper virus to X-irradiated old-adult B6 mice, resulted in an efficient rescue of lymphomagenic viruses, enriched with phenotypically mixed, dualtropic viruses. Some of these DT viral preparations were cloned and seemed to consist mainly of xenotropic sequences. Thus, inoculation of helper viruses influenced the generation and selection of DT viruses. Such viral preparations, enriched with DT viruses, had a better lymphomagenic activity compared to endogenous ecotropic viruses, isolated from radiation-induced RCN-bearing mice. Indirect evidence suggested the involvement of a defective (xenotropic and possibly adjacent cellular genes) particle in lymphoma induction. To conclude, a possible mechanism for the development of radiation-induced RCN is suggested, emphasizing the role of MO in such a process.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D002478:Cells, Cultured; D005260:Female; D006378:Helper Viruses / Q000302:isolation & purification; D016403:Lymphoma, Large B-Cell, Diffuse / Q000276:immunology* / Q000382:microbiology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000276:immunology / Q000382:microbiology; D009381:Neoplasms, Radiation-Induced / Q000276:immunology* / Q000382:microbiology; D013154:Spleen / Q000276:immunology*