[Immunocytochemical localization in human spermatozoa of tubulin and the associated high molecular weight proteins MAP1 and MAP2].
By immunofluorescent and immunoperoxidase techniques and with the use of highly purified antibodies, tubulin and its associated proteins, MAP1 and MAP2 have been identified and precisely localized in human spermatozoa. In relation to well known structural elements, tubulin appeared localized to the region of the axoneme, the centriolar adjunct and the equatorial part of the acrosome, in contrast MAP1 and MAP2 were localized to the post-nuclear region and in the fibrous sheath of the principal piece of the flagella.
D002467:Cell Nucleus / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008869:Microtubule-Associated Proteins; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D013082:Sperm Tail / Q000032:analysis; D013094:Spermatozoa / Q000032:analysis*; D014404:Tubulin / Q000032:analysis*
D008869:Microtubule-Associated Proteins; D011506:Proteins; D014404:Tubulin
Localisation immunocytochimique dans le spermatozoïde humain de la tubuline et des protéines associées de haut poids moléculaire MAP1 et MAP2.