Localization of high molecular weight microtubule-associated proteins (MAP1 and MAP2) in a HeLa microtubule-organizing centre.
With a rabbit antiserum specific for high molecular weight microtubule-associated proteins (HMW/MAPs: MAP1 and MAP2), centrosomes in interphase and mitotic HeLa cells were visualized by indirect immunofluorescence. Pre-immune serum did not stain a similar organelle. Microtubules were observed to originate from the centrosomes, demonstrating their microtubule organizing (MTOC) function. The MTOC antigen(s) to which the anti-HMW/MAP antiserum reacted was resistant to Colcemid, Triton-X-100, Nonidet P-40 and Brij 35. Immunofluorescence localization of the MTOCs was blocked by pre-incubation of the antiserum with a MAP1--MAP2 preparation but not with tubulin, actin, or a 58 K intermediate filament protein.
D003703:Demecolcine / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D007399:Interphase; D008869:Microtubule-Associated Proteins; D008870:Microtubules; D008938:Mitosis; D009940:Organoids / Q000032:analysis* / Q000648:ultrastructure; D011506:Proteins / Q000032:analysis*
D008869:Microtubule-Associated Proteins; D011506:Proteins; D003703:Demecolcine