Methane formation from fructose by syntrophic associations of Acetobacterium woodii and different strains of methanogens.
When Acetobacterium woodii was co-cultured in continuous or in stationary culture with Methanobacterium strain AZ, fructose instead of being converted to 3 mol of acetate was converted to 2 mol of acetate and 1 mol each of carbon dioxide and methane, showing that interspecies hydrogen transfer occurred. In continuous culture the organisms formed a close physical association in clumps; the doubling time for each organism was 6 h at 33 degrees C. Methane mainly was derived from carbon positions 3 and 4 of the sugar, but other carbons also yielded methane; this was shown to be due to carbon dioxide-acetate exchange reactions by A. woodii in a manner similar to that carried out by Clostridium thermoaceticum. Four other methanogens, Methanobacterium M.o.H. and M.o.H. G, Methanobacterium formicicum, and Methanosarcina barkeri (not acetate-adapted) also produced similar results, when co-cultured with A. woodii.
D000091:Acetobacter / Q000254:growth & development / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D019605:Euryarchaeota / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D005632:Fructose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D008697:Methane / Q000096:biosynthesis*; D013045:Species Specificity
D002245:Carbon Dioxide; D005632:Fructose; D006859:Hydrogen; D005947:Glucose; D008697:Methane