Turnover of proteins in asporogenic Bacillus megaterium. Evidence for a gradual decrease of the turnover rate.
The rate of protein turnover in asporogenic Bacillus megaterium decreases continuously during incubation in a sporulation medium. The capability of equilibration of external amino acids with amino acids in the metabolic pool of non-growing cells was retained for at least 5 h. Leucine, while repressing the synthesis of the exocellular protease, does not significantly influence the course of protein degradation in vivo. Transfer of non-growing cells after 4 h to a fresh sporulation medium does not influence the rate of protein degradation. The gradual decrease of the rate of protein turnover in non-growing cells of the asporogenic variant is thus not an artifact caused by a decreased uptake of amino acids by cells or by conditions under which the protein turnover is determined.
D000596:Amino Acids / Q000378:metabolism; D001410:Bacillus megaterium / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D003470:Culture Media; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D013171:Spores, Bacterial
D000596:Amino Acids; D001426:Bacterial Proteins; D003470:Culture Media; D007930:Leucine