Absence of mutagenic activity of trifluoroethanol and its metabolites in Salmonella typhimurium.
Trifluoroethanol, trifluoroacetaldehyde and trifluoroacetate were found to have no mutagenic activity in the standard Salmonella typhimurium reverse mutation assay (Ames test) using a closed incubation system. Negative results were also obtained when incubation mixtures included 9000 x g supernatant fractions of rat liver or testes homogenates along with an NADPH generating system. Rats were pretreated with a polychlorinated biphenyl mixture to induce biotransforming enzyme activity. These results suggest that the previously reported mutagenic activity of fluroxene is not due to metabolites arising from the trifluoroethyl side of the molecule and that inhibition of spermatogenesis in rats by trifluoroethanol is not mediated through a mutagenic mechanism.
D000818:Animals; D001711:Biotransformation; D000431:Ethanol / Q000031:analogs & derivatives*; D008297:Male; D009153:Mutagens / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012486:Salmonella typhimurium / Q000187:drug effects*; D014270:Trifluoroethanol / Q000378:metabolism / Q000633:toxicity*
D009153:Mutagens; D000431:Ethanol; D014270:Trifluoroethanol