[Dynamic aspects of alpha-actinin in cultured rabbit fibroblasts studied with immunofluorescence].
In this work we study alpha-actinin sites in rabbit fibroblasts in culture. For this purpose we extracted alpha-actinin from rabbit striated skeletal muscle and produced the relative antibodies in sheep. Electrophoresis was performed, on PAA slab-gel and the immunodiffusion test on agarose slab-gel. Antibodies were used for direct and indirect immunofluorescence techniques by TRIC and FITC. We noticed that in young fibroblasts alpha-actinin is concentrated in perinuclear regions, while in adult fibroblasts it is scattered, more or less evenly in the cytoplasm and above all on the plates which link the cell membrane to the substractum. Both the direct immunofluorescence method with antibodies anti-alpha-actinin, marked by TRITC, and the indirect one with sheep IgG, marked by FITC, gave identical results.
D000185:Actinin / Q000032:analysis*; D000818:Animals; D004587:Electrophoresis, Agar Gel; D005347:Fibroblasts / Q000032:analysis; D005455:Fluorescent Antibody Technique; D009124:Muscle Proteins / Q000032:analysis*; D009132:Muscles / Q000166:cytology; D011817:Rabbits; D012756:Sheep; D013997:Time Factors
D009124:Muscle Proteins; D000185:Actinin
Aspetti dinamici dell' alpha-actinina in fibroblasti di coniglio in cultura mediante l'immunofluorescenza.