An antigen detected frequently in human sera with elevated levels of alanine aminotransferase: a potential marker for non-A, non-B hepatitis.
In a search for additional antigens associated with virus-induced human liver disease a radioimmunoassay (RIA) was developed using IgG from sera of a multiply transfused person. Polystyrene beads coated with IgG F(ab)'2 fragments, dinitrophenylated F(ab)'2 fragments and 125I-labelled anti-2,4-dinitrophenyl antibodies (Neurath & Strick, 1979) were used in the RIA. An apparently new antigen or the corresponding antibodies were detected in 155 serum specimens from 35/37 (94%)individuals who developed non-A, non-B hepatitis. The antigen was also present in hepatitis B surface antigen-negative sera of blood donors with normal (13.2%) and elevated levels of alanine aminotransferase (34%). The antigen has an approximate mol. wt. of 45,000, a buoyant density of 1.23 g/ml and an isoelectric point of 7.
D000410:Alanine Transaminase / Q000097:blood*; D000941:Antigens / Q000032:analysis*; D001663:Bilirubin / Q000097:blood; D001782:Blood Donors; D002500:Centrifugation, Isopycnic; D006506:Hepatitis A / Q000276:immunology; D006509:Hepatitis B / Q000276:immunology; D006526:Hepatitis C / Q000276:immunology*; D006525:Hepatitis, Viral, Human / Q000276:immunology*; D006801:Humans; D007526:Isoelectric Point; D008297:Male; D008970:Molecular Weight; D011863:Radioimmunoassay
D000941:Antigens; D000410:Alanine Transaminase; D001663:Bilirubin