Lysosomal hydrolases in long term cultures of human gliomas: total enzyme activities and rate of extracellular accumulation.
A number of investigations have suggested a fundamental role for lysosomal enzymes in growth control of normal cells and in the loss of growth control which occurs in neoplasia and in cell cultures transformed by oncogenic viruses. In this investigation total enzyme activities and rates of extracellular accumulation of enzyme were measured in long term cultures of gliomas and normal human astrocytes. Marked differences were found between the cultures with regard to total enzyme activity. Exocytosis rates of the enzymes also showed differences, with one group of tumours demonstrating low or intermediate rates and another smaller group with high rates. There was no positive correlation between the enzyme profile of the cultures and their tumorigenicity in nude mice.
D000118:Acetylglucosaminidase / Q000378:metabolism; D001254:Astrocytoma / Q000201:enzymology; D001921:Brain / Q000201:enzymology; D002478:Cells, Cultured; D005260:Female; D005333:Fetus; D005910:Glioma / Q000201:enzymology*; D005966:Glucuronidase / Q000032:analysis; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology*; D011247:Pregnancy; D013997:Time Factors; D000520:alpha-Glucosidases / Q000032:analysis; D001616:beta-Galactosidase / Q000378:metabolism; D001617:beta-Glucosidase / Q000032:analysis
D006867:Hydrolases; D000520:alpha-Glucosidases; D001617:beta-Glucosidase; D001616:beta-Galactosidase; D005966:Glucuronidase; D000118:Acetylglucosaminidase