Archaebacterial elongation factor Tu insensitive to pulvomycin and kirromycin.
A spermine-dependent, polyphenylalanine-synthesizing cell-free system having an optimum activity at 75-85 degrees C, has been developed from the extremely thermoacidophilic archaebacterium Caldariella acidophila. The C. acidophila system is totally insensitive to the EF-Tu targeted antibiotics pulvomycin (at 40 degrees C) and kirromycin (at 47-72 degrees C) contrary to control systems derived from both mesophilic (Escherichia coli) and thermoacidophilic (Bacillus acidocaldarius) eubacteria. The archaebacterial EF-Tu-equivalent factor is also immunologically unrelated to eubacterial EF-Tu and does not cross react with antibodies against Escherichia coli EF-Tu. The pulvomycin and kirromycin reactions thus provide new phyletic markers for archaebacterial ancestry.
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001407:Bacillus / Q000235:genetics*; D001419:Bacteria / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D006027:Glycosides / Q000494:pharmacology; D007700:Kinetics; D010444:Peptide Elongation Factor Tu; D010445:Peptide Elongation Factors / Q000378:metabolism*; D011728:Pyridones / Q000494:pharmacology; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D013045:Species Specificity; D013096:Spermine / Q000494:pharmacology
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D001426:Bacterial Proteins; D006027:Glycosides; D010445:Peptide Elongation Factors; D011728:Pyridones; C012521:pulvomycin; D013096:Spermine; D010444:Peptide Elongation Factor Tu; C008536:mocimycin