99mTc labelled human leukocytes. An in vitro functional study.
A new method of human leukocyte labelling is described. 20 ml of human blood are allowed to sediment in a dextran solution and the remaining red blood cells are removed using NH4C1. The leukocyte-rich suspension is incubated with a Sn-pyrophosphate solution, and then labelled, using 20 mCi 99mTc pertechnetate. Labelling efficiency is about 30%. Functional activity was shown to be satisfactory: (1) appearance under electron microscopy was quite normal; (2) Klebsiella ingestion, zymosan-stimulated oxygen consumption and iodination were similar in labelled and unlabelled cells, and (3) chemotaxis revealed no alteration in cell migration.
D002469:Cell Separation; D002470:Cell Survival; D002633:Chemotaxis; D002854:Chromatography, Paper; D003594:Cytoplasmic Granules / Q000502:physiology; D006098:Granulocytes / Q000648:ultrastructure; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D007962:Leukocytes / Q000378:metabolism*; D013667:Technetium
D013667:Technetium