Liquid culture of Rhizopus chinensis for the production of acid protease rhizopuspepsin.
1. Culture conditions for the production of rhizopuspepsin in liquid medium by Rhizopus chinensis have been investigated. 2. Optimum production was achieved in 24 hr shaking culture in 1% bovine serum albumin, 1% corn starch, 0.1% yeast extract and a salt mixture, pH 5. 3. Levels at 24 hr compared in isozyme pattern and in quantity to conventional solid culture on wheat bran.
D016282:Aspartic Acid Endopeptidases; D001426:Bacterial Proteins / Q000302:isolation & purification; D003470:Culture Media; D010450:Endopeptidases / Q000096:biosynthesis*; D012233:Rhizopus / Q000201:enzymology*
D001426:Bacterial Proteins; D003470:Culture Media; D010450:Endopeptidases; D016282:Aspartic Acid Endopeptidases; C035794:rhizopuspepsin