Estimation of bovine colostral immunoglobulins by refractometry.
The total protein of colostral whey from dairy cows as determined by a refractometer were compared with the immune globulin concentration obtained by cellulose acetate membrane electrophoresis and the immunoglobulin (IgA + IgG + IgM) contents determined by radial immunodiffusion. The coefficient of correlation between the results obtained by refractometry and electrophoresis was 0.98 (P < 0.001). The correlation between refractometry and radial immunodiffusion was 0.89 (P < 0.001), while that of electrophoresis and radial immunodiffusion was 0.87 (P < 0.001). Refractometry can be used as a simple, fast and inexpensive indirect method of assessing immunoglobulins in colostral whey.
D000818:Animals; D002417:Cattle / Q000276:immunology*; D003126:Colostrum / Q000276:immunology*; D005260:Female; D005779:Immunodiffusion; D007136:Immunoglobulins / Q000032:analysis*; D012031:Refractometry
D007136:Immunoglobulins