Specific detection of different plasma proteins in gel filtration fractions by solid phase enzymeimmunoassay (EIA).
An enzymeimmunoassay (EIA) is described enabling sensitive and specific immunodetection of different proteins in analytical and preparative fractionation of biological mixtures. The method is based on the ability of polystyrene to bind proteins and polypeptides. Commercially available rabbit antisera are used combined with the use of commercial alkaline phosphatase conjugated anti-rabbit-IgG antibodies. The good assortment of rabbit antisera available makes possible identification of hundreds of proteins in biological samples by the present method.
D000818:Animals; D001798:Blood Proteins / Q000276:immunology / Q000302:isolation & purification*; D002850:Chromatography, Gel; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D007138:Immunoglobulin delta-Chains / Q000276:immunology; D009194:Myeloma Proteins / Q000276:immunology; D011137:Polystyrenes / Q000276:immunology*; D011485:Protein Binding; D011817:Rabbits
D001798:Blood Proteins; D007138:Immunoglobulin delta-Chains; D009194:Myeloma Proteins; D011137:Polystyrenes