Antibody response in lacteal secretions of cows after immunization with various concentrations of staphylococcal and streptococcal antigens.
A total of 55 lactating Holstein cows were randomly allocated to 6 groups. Five of these groups (No. 2 through 6) were inoculated on 2 occasions in the region of the external inguinal lymph node with various concentrations of 3 bacterial antigens. Saline solution was administered to group 6 as a control. The antigen preparations consisted of a Staphylococcus aureus bacterin, a Streptococcus agalactiae bacterin, and staphylococcal alpha-toxoid. These antigens were administered as a composite preparation suspended in saline solution. The concentration of antibody in the lacteal secretions, represented by immunoglobulin G specific for each of the 3 vaccine antigens, was monitored during the 18-week experimental period by enzyme-linked immunosorbent assay. The concentration of each of the 3 vaccine components which was required to stimulate a maximal immune response in the lactating gland appears to have been established.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000032:analysis; D007774:Lactation; D008414:Mastitis, Bovine / Q000517:prevention & control; D008892:Milk / Q000276:immunology*; D011247:Pregnancy; D013211:Staphylococcus aureus / Q000276:immunology; D013292:Streptococcus agalactiae / Q000276:immunology; D014121:Toxoids / Q000276:immunology; D014611:Vaccination / Q000662:veterinary*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D014121:Toxoids