Host defenses in experimental scrub typhus: mapping the gene that controls natural resistance in mice.
Natural resistance of mice to lethal ifections of Rickettsia tsutsugamushi, strain Gilliam, is controlled by a single, autosomal, dominant gene, which we have designated Ric, with r and s representing the resistant nd susceptible alleles, respectively. Using three sets of recombinant inbred mouse strains (BXD, BXH, and BXJ), the Ric locus was mapped to Chromosome 5 closely linked to the retinal degeneration (rd) locus. This linkage was confirmed by a backcross analysis. Based on the RI strains and the C57BL/6Ty-le congenic strain (the only proven Ric-rd cross-over), we estimate the recombination frequency between Ric and rd to be 0.015. Three presumptive Ric-rd recombinants detected among 93 backcross mice may represent caes of incomplete penetrance of the resistance allele rather than recombination. Analyis of th C57BL/6JTy-le congenic strain indicates that Ric is proximal to rd on Chromosome 5. If so, the correct gene order is Pgm-1-W-Ric-rd-Gus.
D000818:Animals; D002874:Chromosome Mapping; D002905:Chromosomes, Human, 4-5; D003433:Crosses, Genetic; D005260:Female; D005796:Genes; D006801:Humans; D007113:Immunity, Innate; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D012285:Orientia tsutsugamushi / Q000276:immunology; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic; D012612:Scrub Typhus / Q000235:genetics*