A simple method of labelling mouse Thy-1 antibodies with FITC.
Unlike antibodies produced in rabbits and other animals, mouse antibodies have proved to be difficult to covalently label with fluorochromes. By careful control of the labelling conditions, a method has been established for labelling mouse Thy-1 antibodies obtained by immunisation with normal thymus cells. The resultant FITC Thy-1 antibodies suffer little loss in cytotoxic properties, react with the correct specificity and can be used to label Thy-1 positive cells.
D000818:Animals; D000961:Antilymphocyte Serum / Q000276:immunology / Q000302:isolation & purification; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique / Q000379:methods*; D007519:Isoantigens / Q000502:physiology*; D008214:Lymphocytes / Q000145:classification / Q000276:immunology*; D051379:Mice; D008806:Mice, Inbred AKR; D013861:Thiocyanates; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology
D000961:Antilymphocyte Serum; D005452:Fluoresceins; D007519:Isoantigens; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate