A single platelet aggregometric assay for pro-and antiaggregatory prostanoids.
A method is described for characterizing disaggregatory and proaggregatory prostanoids in a single assay. This "post-addition assay" consists of adding the test agent to PRP containing PGI2 (4-6 nM) 90 sec after an ADP challenge. The relative potencies of known inhibitors of ADP-induced aggregation were identical to the relative disaggregatory potencies determined by post-addition. The proaggregatory relative potencies for 16, 16-Me2-PGE2,PGE2,PGH2 and 11 alpha,9 alpha-epoxymethano-15 alpha-hydroxy-prostadienoic acid were 5.8 :2 : 1.8 : 1, respectively, revealing the significant thrombosis promoting activity of PGE2 and its analogs.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D011464:Epoprostenol / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology*; D006801:Humans; D008722:Methods; D010974:Platelet Aggregation; D011458:Prostaglandins E / Q000494:pharmacology; D011466:Prostanoic Acids / Q000494:pharmacology*
D005227:Fatty Acids; D011458:Prostaglandins E; D011466:Prostanoic Acids; D000244:Adenosine Diphosphate; D011464:Epoprostenol