[Method for the simultaneous determination of aflatoxins B1 and M1 in the liver of slaughter animals].
The method used by Egmond et al. (1979) was chosen from a number of methods recommended for the determination of aflatoxins in raw materials of animal origin. This method is described in detail to facilitate its use in practice in cases of forced slaughters of animals suspected of aflatoxicosis. The sensitivity of the method for aflatoxin B1 and M1 is about 0.05 micrograms . kg-1. On the whole, 88 liver samples were examined for the presence of the aflatoxins B1 and M1. The samples had been obtained from the slaughtered pigs (52), bulls (23), cows (6), calves (5), and from deceased pigs (2). Positive findings were obtained in five of sixteen pork liver samples and in two of the tested five samples of calf liver. All of these animals were suspected of aflatoxicosis. At current slaughter, only one case of 36 examined pigs was found to be positive. Aflatoxin M1 was found more frequently than aflatoxin B1. However, the findings never exceeded the concentration of 100 ng . kg-1; this is less by 1.5 orders than the proposed tolerated concentration for adult animals (5 micrograms . kg-1).
D000348:Aflatoxins / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D002855:Chromatography, Thin Layer / Q000379:methods*; D008099:Liver / Q000032:analysis*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*; D000096702:Aflatoxin Poisoning
D000348:Aflatoxins
Výbĕr metody pro soucasné zjistování aflatoxinů B1 i M1 v játrech jatecných zvírat.