Idiotypic analysis of anti-GAT antibodies. VII. Common idiotype on hybridoma  antibodies to poly(Glu60 Ala40)
A single DBA/2 mouse, immunized with L-glutamic acid60-L-alanine40 (GA), was used to produce hybridoma cell lines. Seven hybridoma anti-GA antibodies were obtained for idiotypic analyses. Two hybridoma anti-L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) antibodies, preferentially reactive to GA, were studied in parallel. Anti-idiotypic antisera to purified anti-GAT and anti-GA serum antibodies and to hybridoma anti-GA antibodies were analyzed by idiotype binding and inhibition of idiotype binding assays. Five of the nine hybridoma antibodies exhibited common GA-1 idiotypic specificities previously demonstrated on the majority of anti-GA antibodies of inbred mouse strains of differing immunoglobulin heavy chain linkage groups; these hybridoma antibodies also possessed private idiotypic determinants. Two GA-1 negative hybridoma anti-GA antibodies appeared identical by immunochemical criteria, arguing that somatic hybridization does not artifactually generate private idiotypic determinants. The results demonstrate that the common GA-1 idiotype system is associated with a family of nonidentical but idiotypically related antibody molecules present in a single DBA/2 mouse, and these antibodies are part of the "GA-1 idiotypic family".
D000409:Alanine / Q000276:immunology; D000818:Animals; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D005971:Glutamates / Q000276:immunology*; D006168:Guinea Pigs; D006822:Hybrid Cells / Q000276:immunology*; D007106:Immune Sera / Q000494:pharmacology; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007147:Immunoglobulin Light Chains; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010455:Peptides / Q000276:immunology*; D011108:Polymers
D005971:Glutamates; D007106:Immune Sera; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007147:Immunoglobulin Light Chains; D010455:Peptides; D011108:Polymers; C022204:anti-GAT antibodies, idiotypic; C020749:GAT; D000409:Alanine