Regulation of macrophage populations. I. Preferential induction of Ia-rich peritoneal exudates by immunologic stimuli.
The amounts of Ia-positive and -negative macrophages were studied in peritoneal exudates of normal mice or of mice injected with various inflammatory materials, infected with Listeria monocytogenes, or injected with hemocyanin. Ia-negative macrophages predominated in exudates from normal mice or from mice given mineral oil, peptone, thioglycollate, culture media, or endotoxin. Infection with Listeria caused a very marked increase in Ia-positive macrophages. The induction of Ia-positive macrophages by Listeria inoculation resulted in great part from an immune process. The Ia-positive exudates were more readily generated in immune mice given a secondary challenge with heat-killed organisms. Furthermore, immune T cells transplanted together with heat-killed organisms into normal mice resulted in Ia-rich exudates. Injection of hemocyanin also induced Ia-rich exudates involving an immune process. We conclude that an immune reaction involving T cells regulates the Ia phenotype of the exudate macrophage population. The Ia-positive macrophages were Fc and C3 receptor positive and phagocytized latex particles.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology / Q000276:immunology*; D002448:Cell Adhesion; D005260:Female; D006183:H-2 Antigens; D006433:Hemocyanins / Q000276:immunology; D007116:Immunization, Passive; D008089:Listeria monocytogenes / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008899:Mineral Oil / Q000494:pharmacology; D010461:Peptones / Q000494:pharmacology; D010586:Phagocytes / Q000276:immunology; D013864:Thioglycolates / Q000494:pharmacology
D006183:H-2 Antigens; D010461:Peptones; D013864:Thioglycolates; D008899:Mineral Oil; D006433:Hemocyanins