Evidence that the acyl-O-esters are intermediates in the catalysis. The mechanism of rabbit mammary fatty acid synthase.
The sequence acetyl-CoA leads to acetyl-O-enzyme leads to acetyl-S-acyl carrier protein has for the first time been demonstrated directly with a multifunctional (mammalian) fatty acid synthase. This was achieved by blocking of the active-site thiols of rabbit mammary fatty acid synthase with iodoacetamide. The modified enzyme was incubated with [14C]acetyl-CoA to form acetyl-O-enzyme, and acetyl-CoA was removed rapidly by centrifuge desalting. We were then able to demonstrate transfer of the acetyl group from [14C]acetyl-O-enzyme to the pantetheine thiol in a fragment of rabbit mammary fatty acid synthase containing the phosphopantetheine group, and to E. coli acyl carrier protein.
D000105:Acetyl Coenzyme A / Q000378:metabolism; D000213:Acyl Carrier Protein / Q000378:metabolism; D000818:Animals; D004926:Escherichia coli; D064429:Fatty Acid Synthases / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000201:enzymology*; D011817:Rabbits
D000213:Acyl Carrier Protein; D000105:Acetyl Coenzyme A; D064429:Fatty Acid Synthases