Abnormalities of T cells in patients with ulcerative colitis. FACS analysis of peripheral lymphocytes.
Heterologous antisera against human brain tissue and human embryonal thymocytes were used to distinguish the changes in lymphocyte subpopulations accompanying ulcerative colitis. Peripheral lymphocytes from normal individuals and from patients with ulcerative colitis were reacted with these heterologous antibodies, stained with fluoresceinated rabbit anti-human immunoglobulins, and analyzed by fluorescence-activated cell sorter. All of the fluorescence profiles of T cells in ulcerative colitis showed shifts with a marked increase in the population that had high fluorescence intensity. Time-course analysis by fluorescence-activated cell sorter revealed that the grade of fluorescence profile shift varied in accordance with the activity of the disease. Natural T-cell toxic autoantibody was found in 27 of the 35 patients with ulcerative colitis. Natural T-cell toxic autoantibodies from these patients reacted with 20% of peripheral T cells without Fc receptors. The abnormality of peripheral blood lymphocytes in ulcerative colitis may be caused by the production of autoantibodies.
D000954:Antigens, Surface / Q000302:isolation & purification; D001323:Autoantibodies / Q000302:isolation & purification; D003093:Colitis, Ulcerative / Q000276:immunology*; D003584:Cytological Techniques; D005260:Female; D005453:Fluorescence; D006801:Humans; D007106:Immune Sera; D008297:Male; D011961:Receptors, Fc / Q000276:immunology; D013601:T-Lymphocytes / Q000473:pathology*
D000954:Antigens, Surface; D001323:Autoantibodies; D007106:Immune Sera; D011961:Receptors, Fc