Mutagenicity of DNOC in Drosophila melanogaster.
The insecticide/ascaricide dinitro-ortho-cresol (DNOC) was tested for mutagenicity in BASC or Muller-5 test with Drosophila melanogaster. The frequencies of induced sexlinked recessive lethals in premeiotic and postmeiotic germ cell stages of spermatogenesis were determined after exposure of adult wild-type males by oral application of DNOC. Sex-linked recessive lethals were scored in the F2 generation according to standard procedures. The mutagenic activity of DNOC at concentrations of 0.25 to 1.01 mM (50-200 ppm) in postmeiotic germ cell stages (11 lethals/1570 chromosomes tested = 0.70%) > premeiotic stages (3/1001 = 0.29%). The mutations rate in all germ cell stages was 0.59% (18/3140). The lowest effective concentration for the induction of recessive lethals in nature sperm was 0.25 mM which is 1/3 to 1/2 LD50 which is approximately 0.61 mM. The frequency of spontaneously occurring lethality was 0.06 (1/1718). The data presented here show significant differences in Kastenbaum-Bowman test. Dimethylnitrosamine (6.8 mM = 500 ppm) induced a rate of 21.0% of lethals (112/534).
D000818:Animals; D003408:Cresols / Q000633:toxicity*; D004138:Dinitrocresols / Q000633:toxicity*; D004331:Drosophila melanogaster; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens; D010575:Pesticides / Q000633:toxicity*
D003408:Cresols; D004138:Dinitrocresols; D009153:Mutagens; D010575:Pesticides; C024132:4,6-dinitro-o-cresol