Nucleotide sequence of initiator tRNA from Bacillus subtilis.
Initiator methionine tRNA, tRNAfMet, was purified from Bacillus subtilis W168 by the use of two column chromatographies on DEAE-Sephadex A-50 and BD-cellulose. The nucleotide sequence was determined to be pC-G-C-G-G-G-G-U-G-G-A-G-C-A-G-U-U-C-G-G-D-A-G-C-U-C-G-U-C-G-G-G-C-U-C-A-U-A-A-C-C-C-G-A-A-G-G-U-C-G-C-A-G-G-T-psi-C-A-A-A-U-C-C-U-G-C-C-C-C-C-G-C-A-A-C-C-AOH. This TRNAfMet exhibited a melting temperature 8 degrees C lower than that of E. coli tRNAfMet in the presence of 0.01 M magnesium acetate.
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D001412:Bacillus subtilis / Q000032:analysis* / Q000201:enzymology; D001483:Base Sequence; D004926:Escherichia coli / Q000032:analysis; D009843:Oligoribonucleotides / Q000032:analysis; D012343:RNA, Transfer / Q000302:isolation & purification; D006163:Ribonuclease T1; D012260:Ribonucleases; D013045:Species Specificity
D009843:Oligoribonucleotides; D012343:RNA, Transfer; D012260:Ribonucleases; D006163:Ribonuclease T1; D000604:Amino Acyl-tRNA Synthetases