Lymphocyte transformation by insulin and insulin-zinc suspensions.
The lymphocyte transformation response to the mitogen phytohaemagglutinin (PHA) was determined in 15 well controlled insulin-dependent diabetics (IDD) with a history of insulin allergy or an acute insulin allergy. There was no significant difference in the PHA response of IDD and normal subjects matched in respect of age and sex. The response of peripheral blood lymphocytes to insulin (Actrapid) and an insulin zinc suspension (Monotard) was also determined. Fifty-three percent of IDD gave a positive reaction to Actrapid. Monotard produced positive reactions both in IDD and normal subjects. In normal subjects, a close correlation between the stimulation indices of Monotard and PHA was found (r = 0 . 966) suggesting that these stimulations depend on a common parameter namely, the reactivity to mitogens.
D000293:Adolescent; D000328:Adult; D000368:Aged; D002712:Chlorides; D003920:Diabetes Mellitus / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004342:Drug Hypersensitivity / Q000276:immunology; D006801:Humans; D007328:Insulin / Q000009:adverse effects / Q000494:pharmacology*; D049528:Insulin, Long-Acting / Q000494:pharmacology*; D061389:Insulin, Regular, Pork; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000276:immunology; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology*; D017967:Zinc Compounds
D002712:Chlorides; D007328:Insulin; D049528:Insulin, Long-Acting; D061389:Insulin, Regular, Pork; D010835:Phytohemagglutinins; D017967:Zinc Compounds; C016837:zinc chloride; C000006:insulin, neutral; D015032:Zinc