Interaction of Lotus-tetragonolobus Lectin (LTL) and an MIF-like factor with guinea-pig macrophages. I. Effects on macrophage migration inhibition and receptor-binding studies.
Purified Lotus-tetragonolobus lectin (LTL) was studied for its interaction with guinea-pig peritoneal macrophages in the migration-inhibition and spreading-inhibition assay. LTL was found capable of inhibiting macrophage migration in a dose-response relationship similar to that of an MIF-life factor present in rat Zajdela-hepatoma ascites, whereas macrophage spreading was less affected. Mitogenic activity of the fucolectin could be excluded. Both the LTL and MIF-like factor seem to come into action by means of alpha-L-fucopyranosyl residues-containing macrophage surface receptors. Both substances act synergistically in inhibiting migration of macrophages. Since binding studies with 125I-LTL demonstrated competition with the MIF-like factor, it is suggested that the latter and LTL share a common surface receptor, and that optimal occupation densities are required for the realization of the inhibitory effect. Possibly, LTL acts in a monomeric form in the migration inhibition assay.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002464:Cell Migration Inhibition; D004306:Dose-Response Relationship, Immunologic; D006168:Guinea Pigs; D007122:Immunoelectrophoresis; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology*; D008263:Macrophage Migration-Inhibitory Factors / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*
D037102:Lectins; D008263:Macrophage Migration-Inhibitory Factors