Enhancement of the chemotactic response of bovine polymorphonuclear leukocytes by levamisole.
The effects of levamisole on random migration, chemotaxis, phagocytosis, and intracellular killing by bovine polymorphonuclear leukocytes (PMN) were investigated. Chemotactic assays were performed, using Micropore filters in modified Boyden chambers. Phagocytosis and intracellular killing of Escherichia coli were analyzed by standard viable bacterial counts. Results indicated that levamisole enhanced the chemotactic response of PMN at concentrations ranging from 10(-4) to 5 x 10(-3)M. Phagocytes collected from cows at 90 minutes after IM injection of levamisole also showed enhanced random migration and chemotaxis. Freshly prepared serum was shown to enhance the levamisole-induced stimulation of chemotaxis. Levamisole had no effect on phagocytosis or intracellular killing of E coli by bovine PMN.
D000818:Animals; D001770:Blood Bactericidal Activity / Q000187:drug effects*; D002417:Cattle / Q000097:blood*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D007273:Injections, Intramuscular / Q000662:veterinary; D007978:Levamisole / Q000008:administration & dosage / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D010587:Phagocytosis / Q000187:drug effects*
D007978:Levamisole