Alcohol dehydrogenase isozymes in the mouse: genetic regulation, allelic variation among inbred strains and sex differences of liver and kidney A2 isozyme activity.
Genetic analysis of a proposed cis-acting temporal locus (Adh-3t), which regulates alcohol dehydrogenase C2 (ADH-C2) activity in mouse epididymis extracts, among F1 (ddN X BALB/c) X ddN male backcross progeny provided evidence for genetic distinctness between the structural (Adh-3) and temporal (Adh-3t) loci on chromosome 3. Genetic analysis also confirmed the close linkage of Adh-1 (encoding liver and kidney ADH-A2) and Adh-3 (encoding stomach ADH-C2) to within 0.3 centimorgans on the mouse genome. Evidence is presented for a proposed closely linked cis-acting temporal locus (designated Adh-lt) for the A2 isozyme (encoded by Adh-1) controlling the activity of this enzyme in mouse kidney extracts, but having no apparent affect on liver and intestine ADH-A2 activities. An extensive survey of the distribution of Adh-1, Adh-3 and Adh-3t alleles among 65 strains of mice is reported--with the exception of two Japanese strains (ddN and KF), linkage disequilibrium between Adh-3 and Adh-3t was observed. Sex differences in mouse liver and kidney ADH-A2 activities were observed, with male/female ratios of approximately 0.6 and 3 respectively for these tissue extracts.
D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000235:genetics*; D000483:Alleles; D000818:Animals; D003433:Crosses, Genetic; D004822:Epididymis / Q000201:enzymology; D005260:Female; D005796:Genes; D014644:Genetic Variation; D007527:Isoenzymes / Q000235:genetics*; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics; D008808:Mice, Inbred CBA / Q000235:genetics; D008815:Mice, Inbred Strains / Q000235:genetics*; D013045:Species Specificity; D013270:Stomach / Q000201:enzymology
D007527:Isoenzymes; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase