Enzyme inhibitors for renin assay in rat plasma.
1. Degradation of 125I-labelled [Ile5]-angiotensin I and unlabelled angiotensin I was studied during incubation of rat plasma treated with disodium ethylenediaminetetra-acetate (disodium EDTA) at 37 degrees C, pH 6.5, for 1 h with 8-hydroxyquinoline, phenylmethylsulphonyl fluoride or di-isopropyl fluorophosphate. 2. Isoelectric focusing showed approximately 100, 59--66 and 10--31% preservation of 125I-labelled angiotensin I during incubation with 8-hydroxyquinoline (5.0 mmol/l), phenylmethylsulphonyl fluoride (15 mmol/l) and di-isopropyl fluorophosphate (1.09 mmol/l) respectively. Mean recovery of unlabelled angiotensin I was 102, 77 and 32% with 8-hydroxyquinoline, phenylmethylsulphonyl fluoride and di-isopropyl fluorophosphate respectively. 3. 8-Hydroxyquinoline appears the preferable enzyme inhibitor for renin assay in rat plasma.
D000803:Angiotensin I / Q000097:blood; D000818:Animals; D005260:Female; D007525:Isoelectric Focusing; D007531:Isoflurophate; D015125:Oxyquinoline; D010664:Phenylmethylsulfonyl Fluoride; D011863:Radioimmunoassay; D051381:Rats; D012083:Renin / Q000097:blood*
D007531:Isoflurophate; D010664:Phenylmethylsulfonyl Fluoride; D015125:Oxyquinoline; D000803:Angiotensin I; D012083:Renin