A short-term-culture ELISA system to detect IgG and autoantibody synthesis by human lymphocytes.
A simple combined culture and assay system for small numbers of human lymphocytes is described which permits rapid detection of immunoglobulin adherent to 96-well plates after 18 h culture, by means of an enzyme-linked immunosorbent assay (ELISA). The method is capable of detecting thyroglobulin antibody secreted by cells obtained from fine needle aspiration of the thyroid and therefore allows investigation of B cells activated in vivo from different sites by quantitation of the amount of immunoglobulin or antibody secreted.
D000915:Antibody Affinity; D000921:Antibody-Producing Cells / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis* / Q000096:biosynthesis; D002452:Cell Count; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006462:Hemolytic Plaque Technique; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000096:biosynthesis; D013205:Staphylococcal Protein A / Q000494:pharmacology; D013954:Thyroglobulin / Q000276:immunology; D013997:Time Factors
D001323:Autoantibodies; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D013954:Thyroglobulin; D003513:Cycloheximide