Effect of aluminum chloride and zinc sulfate on Autographa california nuclear polyhedrosis virus (ACNPV) replication in cell culture.
When IPL-SF-21AE III continuous insect cell line was grown and maintained in IPL-41 insect cell culture medium supplemented with 16 microM of AlCl3 or 0.24 microM of ZnSO4 . 7H2O, or both metallic salts, and then infected with Autographa california nuclear polyhedrosis virus, virus replication was increased significantly. The yield of polyhedral inclusion bodies (PIB) was enhanced up to 121%. Synthesis of cell-free nonoccluded virus was increased to 365% when infectivity was assayed by the plaque method. Newly applied electron microscopic quantitation and stereological techniques also revealed a significant increase in virus particles (VP) and in amount and size of PIB as well as number of VP per PIB.
D000535:Aluminum / Q000494:pharmacology*; D000077410:Aluminum Chloride; D017607:Aluminum Compounds; D000818:Animals; D002460:Cell Line; D002712:Chlorides; D007181:Inclusion Bodies, Viral / Q000187:drug effects; D007304:Insect Viruses / Q000187:drug effects*; D007313:Insecta; D008854:Microscopy, Electron; D010572:Pest Control, Biological; D013431:Sulfates / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*; D015032:Zinc / Q000494:pharmacology*; D019287:Zinc Sulfate
D017607:Aluminum Compounds; D002712:Chlorides; D013431:Sulfates; D000077410:Aluminum Chloride; D019287:Zinc Sulfate; D000535:Aluminum; D015032:Zinc