The effect of uraemic "middle-sized molecules" on T lymphocyte functions.
The activity of the leucocyte inhibitory factor (LIF) stimulated with Concanavalin A was examined in chronic uraemic, haemodialysed patients. It was found that the LIF activity decreased in chronic uraemic patients as compared with the normal controls. The absolute T lymphocyte count and active E rosette formation also decreased. In in vitro experiments the authors have also examined the inhibitory effect of the so-called "middle-sized molecule" (MSM) on uraemic materials separated from the serum and the haemofiltrate. They observed that the materials with a molecular weight of 1000-1500 isolated both from the serum and the haemofiltrate significantly inhibited the Con A stimulated LIF production of normal lymphocytes and decreased the ratio of the active E rosette-forming T lymphocytes. On the basis of their experiments the MSM uraemic materials are considered responsible for the decreased immune reactivity of uraemic patients. These uraemic toxins can be dialysed.
D000328:Adult; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D007961:Leukocyte Migration-Inhibitory Factors / Q000276:immunology*; D008222:Lymphokines / Q000276:immunology*; D008231:Lymphopenia / Q000209:etiology / Q000276:immunology; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis / Q000009:adverse effects; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*; D014118:Toxins, Biological; D014511:Uremia / Q000209:etiology / Q000276:immunology*
D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D014118:Toxins, Biological; C026865:uremia middle molecule toxins; D003208:Concanavalin A