[A system for quality control of biotransformation enzymes in the mucosa of small intestine (author's transl)].
A system for quality control of the determination of arylhydrocarbonhydroxylase (benzpyrenhydroxylase), ethoxycoumarin-O-deethylase, NADPH-cytochrom-c-reductase, epoxidehydrase and uridindiphosphoglucouronyltransferase was developed. We used lyophilized and frozen samples of postmitochondrial supernatant of rat and guinea pig small intestinal mucosa, which was prepared using a 1:5 ratio of mucosa to K-phosphate buffer. For NADPH-cytochrome-c-reductase, the microsomal suspension from rat small intestine was used. The samples were stored at -30 degrees C. The coefficient of variation within run was about 5% (15 samples), the coefficient of variation between run (15 days) about 10%. The control specimens were stable for 6 months.
D015660:7-Alkoxycoumarin O-Dealkylase; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000032:analysis*; D001579:Benzopyrene Hydroxylase / Q000032:analysis; D001711:Biotransformation; D004851:Epoxide Hydrolases / Q000032:analysis*; D014453:Glucuronosyltransferase / Q000032:analysis*; D006168:Guinea Pigs; D007413:Intestinal Mucosa / Q000201:enzymology*; D007421:Intestine, Small / Q000201:enzymology*; D008722:Methods; D008861:Microsomes / Q000201:enzymology; D009251:NADPH-Ferrihemoprotein Reductase / Q000032:analysis*; D010105:Oxygenases / Q000032:analysis*; D011786:Quality Control; D051381:Rats
D010105:Oxygenases; D015660:7-Alkoxycoumarin O-Dealkylase; D001579:Benzopyrene Hydroxylase; D001189:Aryl Hydrocarbon Hydroxylases; D009251:NADPH-Ferrihemoprotein Reductase; D014453:Glucuronosyltransferase; D004851:Epoxide Hydrolases
Ein Qualitätskontrollsystem für Biotransformationsenzyme in der Dünndarmmucosa.