[Comparative analysis of the virion structural proteins of 3 nuclear polyhedrosis viruses].
The composition of structural proteins of virions of nuclear polyhedrosis viruses (NPV) of Barathra brassicae L., Lymantria dispor L. and Aporia crataegi L. was studied by polyacrylamide gel-SDS electrophoresis (PAGE-SDS). The object of the study included fractions of virion bundles in the envelope. All the baculoviruses under study were shown to have in virion structural proteins 23-24 polypeptides with molecular weights ranging from 12500 to 95000 daltons. Alongside with polypeptides of similar molecular weight, each baculovirus was shown to have a specific set of structural proteins allowing them to be readily identified. Specific distribution of major polypeptides of the virions was found. All the protein fractions were readily reproducible in repeated analyses with the exception of two minor polypeptides of NPV of Barathra brassicae L. The study showed that the composition of structural proteins of virions may be an important criterion for identification of baculoviruses under identical electrophoresis conditions.
D004591:Electrophoresis, Polyacrylamide Gel; D007304:Insect Viruses / Q000032:analysis*; D008970:Molecular Weight; D014764:Viral Proteins / Q000032:analysis*; D014771:Virion / Q000032:analysis*
D014764:Viral Proteins
Sravnitel'nyĭ analiz strukturnykh belkov virionov trekh virusov iadernogo poliédroza.