A micromethod for clotting tests and coagulation factor assays.
A simple microtechnique for carrying out partial thromboplastin time with kaolin tests with 2 microliter or less of test plasma is described. For single stage factor assays, less than 1 microliter of test solution may be used. Reagents and test plasma are loaded in sequence into a 10 microliter, long needle syringe and introduced into a micro test-tube immobilized in a water bath. The end-point is taken as a positive clearing of kaolin turbidity from the mixture while stirring. Correlation with normal techniques has been excellent.
D001780:Blood Coagulation Tests / Q000379:methods*; D005164:Factor IX / Q000032:analysis*; D005169:Factor VIII / Q000032:analysis*; D006467:Hemophilia A / Q000175:diagnosis; D002836:Hemophilia B / Q000175:diagnosis; D006801:Humans; D007616:Kaolin; D010314:Partial Thromboplastin Time / Q000379:methods*
D007616:Kaolin; D005169:Factor VIII; D005164:Factor IX