Failure to detect conventional enterotoxins in classical enteropathogenic (serotyped) Escherichia coli strains of proven pathogenicity.
Ammonium sulfate-precipitated supernatants of classical enteropathogenic Escherichia coli strains were negative when investigated for enterotoxin production in rabbit ligated ileal loops, rabbit skin vascular permeability factor tests, suckling mice, and Y-1 adrenal cells. They also failed to stimulate guanylate cyclase activity in homogenates of rabbit, rat, and infant mouse intestines. Furthermore, DNA from enteropathogenic E. coli lacked sequences that encode heat-labile and heat-stable enterotoxins. These studies fail to show conventional enterotoxin synthesis by classical enteropathogenic E. coli.
D000818:Animals; D001427:Bacterial Toxins; D001483:Base Sequence; D001681:Biological Assay; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D029968:Escherichia coli Proteins; D051379:Mice; D011817:Rabbits; D051381:Rats; D012703:Serotyping
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C034151:heat stable toxin (E coli); C027538:heat-labile enterotoxin, E coli