Isolation and properties of membranes from Bacillus megaterium spores.
Membranes from dormant and heat-activated spores of Bacillus megaterium QM B1551 were isolated and purified by gentle lysis procedures followed by differential and sucrose density gradient centrifugations. The purified membranes were enriched for inner membranes and were characterized by their density and content of proteins, phospholipids, enzymes, cytochromes, and carotenoids. These purified spore membranes could be used to investigate their role in the triggering of germination.
D001410:Bacillus megaterium / Q000648:ultrastructure*; D001426:Bacterial Proteins / Q000032:analysis; D002458:Cell Fractionation; D003580:Cytochromes / Q000032:analysis; D007425:Intracellular Membranes / Q000032:analysis*; D008563:Membrane Lipids / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D010088:Oxidoreductases / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D013171:Spores, Bacterial / Q000648:ultrastructure*
D001426:Bacterial Proteins; D003580:Cytochromes; D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids; D010088:Oxidoreductases