Cleavage of human placental lactogen precursor by an enzyme from microbial membranes.
When membranes from Escherichia coli MRE 600 are added to an in vitro translation system, they are able to cleave correctly prehuman placental lactogen to yield the mature hormone. The protein was identified by SDS/polyacrylamide-gel electrophoresis and by determining its amino acid sequence. These studies were aided by the development of a new method for the separation of human placental lactogen from its precursor.
D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000201:enzymology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008566:Membranes / Q000201:enzymology; D010920:Placenta / Q000378:metabolism; D010928:Placental Lactogen / Q000378:metabolism*; D011247:Pregnancy; D011498:Protein Precursors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors
D011498:Protein Precursors; D012333:RNA, Messenger; D010928:Placental Lactogen