Age-dependent changes in brain protein synthesis in the rat.
Brain protein synthesis was studied in vivo, in brain slices, and in cell-free systems in rats aged 1, 16, and 24 months. We observed a highly significant reduction in amino acid incorporation with advancing age. This reduction was observed in vivo, in slices, in postmitochondrial supernatant, microsomes, and membrane-bound polysomes. Free heavy polysomes showed no age-dependent decline but formed a smaller proportion of total ribosomes in older animals. These studies suggest that in the rat brain protein synthesis declines before senescence, possibly due to an impairment in the initiation process.
D000367:Age Factors; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002474:Cell-Free System; D066298:In Vitro Techniques; D008239:Lysine / Q000378:metabolism; D008297:Male; D008861:Microsomes / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D011132:Polyribosomes / Q000378:metabolism; D051381:Rats
D009419:Nerve Tissue Proteins; D008239:Lysine