Organization and complete sequence of identical embryonic and plasmacytoma kappa V-region genes.
We have determined the entire sequence of two immunoglobulin kappa variable region genes derived separately from embryonic and plasmacytoma cells. Both genes are in the germline configuration; both are organized into hydrophobic leader and mature V-region coding sequences separated by a short intervening sequence. Both are identical with one another throughout the 1360 bases determined. In addition, there is a simple repeat sequence of 31 CA doublets that occurs about 300 bases from the 3' side of the coding sequence in each gene.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001666:Binding Sites, Antibody; D002460:Cell Line; D004247:DNA; D004273:DNA, Neoplasm; D004355:Drug Stability; D004622:Embryo, Mammalian; D005796:Genes; D005815:Genetic Code; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis*; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains / Q000096:biosynthesis*; D051379:Mice; D010954:Plasmacytoma; D014176:Protein Biosynthesis
D004273:DNA, Neoplasm; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D004247:DNA