A novel non-separation fluoroimmunoassay for thyroxine.
It has proved possible to develop a novel non-separation fluoroimmunoassay for thyroxine employing antibodies both to thyroxine and to fluorescein incorporated into immune complexes by addition of a common species-specific anti-immunoglobulin G serum. The labelled hapten could bind to antibody of either specificity but not to both at the same time. Binding to anti-fluorescein markedly reduced fluorescence whereas binding to anti-thyroxine did not. Unlabelled thyroxine competed with the labelled hapten only for anti-thyroxine binding sites and, as a result, more of the labelled thyroxine was bound to anti-fluorescein with a decrease in total fluorescence.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism; D001666:Binding Sites, Antibody; D005452:Fluoresceins / Q000276:immunology; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000494:pharmacology; D007118:Immunoassay / Q000379:methods; D011817:Rabbits; D012756:Sheep; D013974:Thyroxine / Q000032:analysis* / Q000276:immunology
D000936:Antigen-Antibody Complex; D005452:Fluoresceins; D007106:Immune Sera; D013974:Thyroxine