A critical evaluation of a serum sermucoid assay and its replacement by a serum alpha 1 acid glycoprotein assay.
The composition of serum seromucoid, the protein fraction of serum not precipitated by 0.6 M perchloric acid, has been shown to vary with the technique of preparation. Immunochemical examination revealed that 91.5% of the protein present in the seromucoid fraction of serum was alpha 1 acid glycoprotein, the remainder consisting of alpha 1 antitrypsin, alpha 1 antichymotrypsin, beta 2 glycoprotein, haemopexin, albumin, and pre-albumin. Serum alpha 1 acid glycoprotein concentration determined by radial immunodiffusion correlated well with serum seromucoid concentration although the former was usually 0.4 g/l lower. The determination of serum alpha 1 acid glycoprotein by an immunological method is more precise than the seromucoid method and is not subject to interference from other proteins.
D000293:Adolescent; D000328:Adult; D000418:Albumins / Q000032:analysis; D002918:Chymotrypsin / Q000032:analysis / Q000037:antagonists & inhibitors; D005260:Female; D006466:Hemopexin / Q000032:analysis; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007249:Inflammation / Q000097:blood; D007410:Intestinal Diseases / Q000097:blood; D008297:Male; D008875:Middle Aged; D009961:Orosomucoid / Q000032:analysis*; D011228:Prealbumin / Q000032:analysis; D014361:Trypsin Inhibitors / Q000032:analysis; D000514:alpha 1-Antichymotrypsin; D000515:alpha 1-Antitrypsin / Q000032:analysis
D000418:Albumins; D009961:Orosomucoid; D011228:Prealbumin; D014361:Trypsin Inhibitors; D000514:alpha 1-Antichymotrypsin; D000515:alpha 1-Antitrypsin; D006466:Hemopexin; D002918:Chymotrypsin