High-mannose glycopeptides from embryonal carcinoma cells.
Endo-beta-N-acetylglucosaminidase H released four major oligosaccharides from high-mannose glycopeptides prepared from embryonal carcinoma cells. The oligosacchaides were indistinguishable from (Man)9GlcNAc, (Man)8GlcNAc, (Man)7GlcNAc, and (Man)6GlcNAc isolated from fibroblasts. This result suggests that the biosynthetic pathway of asparagine-linked oligosaccharides in early embryonic cells is controlled as in adult cells, at least to the initial stage of processing of the nascent oligosaccharide transferred from lipid-linked intermediate.
D000117:Acetylglucosamine / Q000032:analysis; D000118:Acetylglucosaminidase; D002478:Cells, Cultured; D006020:Glycopeptides / Q000032:analysis*; D008358:Mannose / Q000032:analysis*; D008361:Mannosidases; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D009844:Oligosaccharides / Q000032:analysis; D013724:Teratoma / Q000378:metabolism*
D006020:Glycopeptides; D009844:Oligosaccharides; D008361:Mannosidases; D000118:Acetylglucosaminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008358:Mannose; D000117:Acetylglucosamine