[Immunological and electrophoretic characterization of flagellins of different H-types of Pseudomonas aeruginosa (author's transl)].
P. aeruginosa flagella of the complex serotype a (a0, a2, a3) and the uniform serotype b were isolated, purified, and depolymerized by heat. The flagellins obtained form one precipitin band each and show reactions of non-identity in Ouchterlony tests. The immunologic reaction is not altered by treatment with SDS and mercaptoethanol. In disc microelectrophoresis and SDS gradient gel microelectrophoresis on polyacrylamide gels, the flagellins migrate as homogeneous proteins. They have identical electrophoretic mobilities and a molecular weight of about 50,000.
D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000276:immunology*; D004589:Electrophoresis, Disc; D004591:Electrophoresis, Polyacrylamide Gel; D005408:Flagellin / Q000032:analysis / Q000276:immunology*; D005779:Immunodiffusion; D008623:Mercaptoethanol / Q000494:pharmacology; D008970:Molecular Weight; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000276:immunology*; D012703:Serotyping; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D005408:Flagellin; D012967:Sodium Dodecyl Sulfate; D008623:Mercaptoethanol
Immunologische und elektrophoretische Charakterisierung der Flagelline unterschiedlicher H-Typen von Pseudomonas aeruginosa.