Temperature-mediated hyperinduction of fatty acid desaturation in pre-existing and newly formed fatty acids synthesized endogenously in Bacillus megaterium.
3H-labedeled fatty acids synthesized endogenously by Bacillus megaterium ATCC 14581 growing at 35 degrees C in the presence of L-[G-3H]valine exhibited the same time-course of hyperinduced desaturation following a temperature decrease to 20 degrees C as was observed previously with exogenously supplied 14C-labeled fatty acids. Radioactive fatty acids synthesized in the presence of [U-14C]glucose during hyperinduction at 20 degrees C following a shift-down from 35 degrees C were desaturated at the same relative rate as 14C-labeled fatty acids synthesized previously at 35 degrees C, suggesting that the newly synthesized fatty acids equilibrate with a large portion of the preexisting moieties before becoming susceptible to desaturation.
D001410:Bacillus megaterium / Q000378:metabolism*; D003080:Cold Temperature; D004790:Enzyme Induction; D044943:Fatty Acid Desaturases / Q000096:biosynthesis; D005227:Fatty Acids / Q000096:biosynthesis*; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D005947:Glucose / Q000378:metabolism; D014633:Valine / Q000378:metabolism
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D044943:Fatty Acid Desaturases; D014633:Valine; D005947:Glucose