Frequent occurrence of anti-rabbit IgM in IgA deficiency.
Analysis of IgA deficient sera revealed impaired hemolysis of sensitized sheep erythrocytes when tested by a hemolysis in gel (HIG) assay developed for detection of complement deficiencies. All sera were normal in a test for the alternative pathway. The impaired hemolysis was not related to complement aberrations but was caused by antibodies to rabbit IgM, demonstrated in 14 of 21 IgA deficient sera, by use of HIG technique and by agglutination. The presence of these antibodies was not related to age, sex or disease. One serum was further examined and the antibodies were shown to be of IgG class.
D000328:Adult; D000361:Agammaglobulinemia / Q000276:immunology*; D000368:Aged; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D001667:Binding, Competitive; D002648:Child; D003170:Complement Pathway, Alternative; D003165:Complement System Proteins / Q000172:deficiency; D005260:Female; D006462:Hemolytic Plaque Technique; D006801:Humans; D017098:IgA Deficiency; D007075:Immunoglobulin M / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D007075:Immunoglobulin M; D003165:Complement System Proteins