Observations on the vimentin-10-NM filaments during mitosis in BHK21 cells.
Mitotic BHK21 cells were examined by immunofluorescence optical sectioning after staining with anti-vimentin. Throughout all stages of mitosis, intact 10-nm filaments were observed to form a cage around the mitotic spindle. These observations were confirmed by serial section electron microscopy which demonstrated assembled 10-nm filaments in the cytoplasm surrounding the spindle. Therefore, in contrast to previous reports, mitotic BHK21 cells show a similar cage-like 10-nm filament arrangement, as found in a variety of other cell types.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003593:Cytoplasm / Q000648:ultrastructure; D003599:Cytoskeleton / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D007381:Intermediate Filament Proteins / Q000032:analysis*; D007668:Kidney; D008854:Microscopy, Electron; D008938:Mitosis; D014746:Vimentin
D007381:Intermediate Filament Proteins; D014746:Vimentin