Inhibition of fertilization in the hamster by sodium aurothiomalate, a hyaluronidase inhibitor.
Sodium aurothiomalate, a low molecular weight inhibitor of hyaluronidase, blocked in-vitro fertilization in hamsters at the level of the zona pellucida: concentrations of 25-250 micrograms inhibitor/ml prevented fertilization of cumulus-intact and cumulus-denuded eggs. Fertilization of zona-free ova was not affected. In-vivo fertilization was also reduced (from 100% controls to 37.5%) by 10 mg inhibitor/ml added to an epididymal sperm suspension before artificial insemination into the uterus. These findings suggest that hyaluronidase may play a role in zona penetration or that sodium aurothiomalate blocks sperm penetration of the zona by inhibiting an enzyme(s) other than hyaluronidase.
D000818:Animals; D006224:Cricetinae; D005260:Female; D005306:Fertilization / Q000187:drug effects; D005307:Fertilization in Vitro / Q000187:drug effects*; D006052:Gold Sodium Thiomalate / Q000494:pharmacology*; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors*; D008297:Male; D008647:Mesocricetus; D013094:Spermatozoa / Q000187:drug effects; D015044:Zona Pellucida / Q000187:drug effects
D006052:Gold Sodium Thiomalate; D006821:Hyaluronoglucosaminidase