Properties of the non-specific calcium-binding sites of rabbit skeletal-muscle myosin.
The non-specific Ca2+-binding sites of skeletal-muscle myosin are located on the light chains; with the dissociation of light chains there is a corresponding decrease in the number of Ca2+-binding sites on light-chain-deficient myosin. The released light chains have a decreased binding affinity. Myosin heavy chains indirectly influence the Ca2+-binding properties of light chains by increasing the affinity of light chains for bivalent cations; this influence varies with pH. Because of light-chain dissociation at low Ca2+ and/or Mg2+ concentrations, anomalies may exist when analyses of non-specific Ca2+-binding properties of myosin are assessed by dialysis equilibrium.
D000818:Animals; D001665:Binding Sites; D002118:Calcium; D002135:Calcium-Binding Proteins; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium; D009218:Myosins; D011817:Rabbits
D002135:Calcium-Binding Proteins; D004492:Edetic Acid; D009218:Myosins; D008274:Magnesium; D002118:Calcium