15N NMR study of a mixture of uniformly labeled tRNAs.
15N NMR spectra were taken of 15N-enriched tRNA extracted from bakers yeast; ammonium sulfate was used as a nitrogen source. The increase in the degree of denaturation of tRNA, which occurs with increase in temperature from 30 degrees C to 70 degrees C, resulted in no large changes in 15N chemical shifts at acidic and neutral pH but quite pronounced changes in proton-15N nuclear Overhauser effects.
D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D012331:RNA, Fungal; D012343:RNA, Transfer; D012441:Saccharomyces cerevisiae
D012331:RNA, Fungal; D012343:RNA, Transfer