A simple and rapid immunoenzymatic assay of prostatic acid phosphatase in serum.
A method is described for the quantitative assay of human prostatic acid phosphatase after its isolation from serum by a double antibody technique employing a specific rabbit antiserum and a goat anti-rabbit gammaglobulin serum. After centrifugation, the precipitate is analyzed for phosphatase activity using p-nitrophenylphosphate as substrate. The method allows a rapid, precise, specific and sensitive assay.
D000135:Acid Phosphatase / Q000097:blood* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D008297:Male; D011467:Prostate / Q000201:enzymology*
D007106:Immune Sera; D000135:Acid Phosphatase