Binding of 125I-labeled endotoxin to bovine, canine, and equine platelets and endotoxin-induced agglutination of canine platelets.
Endotoxin from Escherichia coli O127:B8, Salmonella abortus-equi and S minnesota induced clumping of some canine platelets (PLT) at a final endotoxin concentration of 1 microgram/ml. Endotoxin-induced clumping of canine PLT was independent of PLT energy-requiring processes, because clumping was observed with canine PLT incubated with 2-deoxy-D-glucose and antimycin A. The PLT responded to adenosine diphosphate before, but not after, incubation with the metabolic inhibitors. Endotoxin induced a slight and inconsistant clumping of bovine and equine PLT at high (mg/ml) endotoxin concentration. High-affinity binding sites could not be demonstrated on canine, bovine, and equine PLT, using 125I-labeled E coli O127:B8 endotoxin. Nonspecific binding was observed and appeared to be due primarily to an extraneous coat on the PLT surface that was removed by gel filtration. The endotoxin that was bound to PLT did not appear to modify PLT function. An attempt to identify plasma proteins that bound physiologically relevant amounts of endotoxin was not successful. The significance of the endotoxin-induced clumping or lack of it on the pathophysiology of endotoxemia is discussed.
D000371:Agglutination / Q000187:drug effects; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002417:Cattle / Q000097:blood*; D004285:Dogs / Q000097:blood*; D004731:Endotoxins / Q000378:metabolism / Q000494:pharmacology*; D004926:Escherichia coli; D006736:Horses / Q000097:blood*; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D010974:Platelet Aggregation / Q000187:drug effects; D012475:Salmonella
D004731:Endotoxins; D007457:Iodine Radioisotopes