PEP4 gene function is required for expression of several vacuolar hydrolases in Saccharomyces cerevisiae.
The pep4-3 mutation results in a 90-95% reduction in the levels of five vacuolar hydrolases in yeast, including proteinases A and B, carboxypeptidase Y, RNase(s) and the repressible alkaline phosphatase. The mutation is without effect on two secreted glycoproteins, on an enzyme of the vacuolar membrane, and on a proteinase located outside of the vacuole. Mutations at the PEP4 locus exhibit a dosage effect on the levels of some, but not all, of the enzymes whose expression requires the function of the gene.
D000469:Alkaline Phosphatase / Q000235:genetics; D002268:Carboxypeptidases / Q000235:genetics; D003594:Cytoplasmic Granules / Q000201:enzymology; D005796:Genes; D005800:Genes, Fungal; D005809:Genes, Regulator; D006026:Glycoside Hydrolases / Q000235:genetics; D010447:Peptide Hydrolases / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D012260:Ribonucleases / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D043324:beta-Fructofuranosidase
D012260:Ribonucleases; D000469:Alkaline Phosphatase; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D002268:Carboxypeptidases; D010447:Peptide Hydrolases