[Determination of IgM anti-Toxoplasma gondii antibodies with the immunoenzymatic (IE) method].
The immunoenzymatic method for the research of specific IgM antibodies anti-Toxoplasma gondii is compared to the traditional methods of dosage: absorption of the IgG on staphylococcus and depolymerization of the IgM with 2-mercaptoethanol. The sensitivity and specificity of this method have been analyzed on a sample of 193 serums in order to consider the probabilities of false positive and negative reactions. The simplicity of method and the possibility of expressing values as reciprocal of the maximum dilution make this an elected method for the research of specific IgM. The reduced quantity of serum necessary in carrying out the test represents such a prerogative as to make this method particularly fit for researches on new-born babies.
D000906:Antibodies / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007126:Immunoglobulin Allotypes; D007075:Immunoglobulin M; D014122:Toxoplasma; D014123:Toxoplasmosis / Q000175:diagnosis / Q000276:immunology
D000906:Antibodies; D007126:Immunoglobulin Allotypes; D007075:Immunoglobulin M
Il dosaggio degli anticorpi IgM anti-"Toxoplasma gondii" con il metodo immunoenzimatico (IE).