[Redox enzymes in the corneal cells after low-temperature preservation (a cytochemical study)].
Activity of LDG, MDG, alpha-GFDG, NAD X H2DG and NADP X H2DG was studied in different type cells of rabbit cornea subjected to cryopreservation (-196 degrees C) in the presence of PEO-400. Fresh cornea was used for comparison. It is revealed that the enzyme activity in the represerved tissue changes: the level of cytochemical reaction increases with MDG and alpha-GFDG detection and decreases with NADP X H2DG detection. The difference in response to cooling may be attributed to unequal sensitivity of structural cell elements to cooling.
D000818:Animals; D003315:Cornea / Q000201:enzymology*; D016039:Corneal Transplantation; D005615:Freezing; D006651:Histocytochemistry; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D011817:Rabbits; D014021:Tissue Preservation
D010088:Oxidoreductases
Sostoianie nekotorykh okislitel'no-vosstanovitel'nykh fermentov v kletkakh rogovitsy posle nizkotemperaturnoĭ konservatsii (tsitokhimicheskoe issledovanie).