Simultaneous localization of [3H]estradiol and neurophysin I or arginine vasopressin in hypothalamic neurons demonstrated by a combined technique of dry-mount autoradiography and immunohistochemistry.
A combined technique of dry-mount autoradiography and immunoperoxidase staining is described for the localization of steroid hormones and neuropeptides in the same brain section. With this procedure [3H]estradiol is localized in nuclei of neurophysin and arginine vasopressin producing cells of the mouse supraoptic and paraventricular nucleus. The results suggest a direct action of estradiol on neurohypophysial hormone producing cells. This immunoautoradiographic technique is suitable for the simultaneous localization of steroid hormones and neuropeptides in the brain.
D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D001345:Autoradiography; D002369:Castration; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007031:Hypothalamus / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D051379:Mice; D009481:Neurophysins / Q000378:metabolism*; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D013495:Supraoptic Nucleus / Q000378:metabolism
D009481:Neurophysins; D001127:Arginine Vasopressin; D004958:Estradiol