Enzyme-linked protein A: an enzyme-linked immunosorbent assay reagent for detecting antibodies in tuberculous exotic animals.
An enzyme-linked immunosorbent assay (ELISA) was developed, using protein A labeled with horseradish peroxidase for detecting antibodies in tuberculous exotic mammals (llamas, rhinoceroses, elephants). The modified ELISA provides a rapid procedure for screening several animal species simultaneously for tuberculosis without the production of specific anti-species conjugates. Heat-killed cells of Mycobacterium bovis and M avium and purifed protein-derivative tuberculin of M bovis were used as antigens for ELISA.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D001188:Artiodactyla / Q000276:immunology*; D002161:Camelids, New World / Q000276:immunology*; D004606:Elephants / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D009163:Mycobacterium bovis / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology; D010527:Perissodactyla / Q000276:immunology*; D013205:Staphylococcal Protein A; D014374:Tuberculin Test; D014376:Tuberculosis / Q000276:immunology / Q000662:veterinary*
D000907:Antibodies, Bacterial; D013205:Staphylococcal Protein A