1H n.m.r. of the DNA-dependent RNA polymerase from Escherichia coli.
The (1)H n.m.r. study of the DNA-dependent RNA polymerase from Escherichia coli has revealed that the holoenzyme (betabeta'alpha(2)sigma) displays two mobile regions: one, observable also in the core enzyme (betabeta'alpha(2)), is characterized by basic amino acids and its appearance and form depend on ionic strength; the other, specific to the holoenzyme, is characterized by threonine residues and its appearance does not depend on ionic strength.
D001051:Apoenzymes / Q000032:analysis*; D001059:Apoproteins / Q000032:analysis*; D012321:DNA-Directed RNA Polymerases / Q000032:analysis*; D004926:Escherichia coli / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D009994:Osmolar Concentration; D011487:Protein Conformation
D001051:Apoenzymes; D001059:Apoproteins; D012321:DNA-Directed RNA Polymerases