Brain gangliosides: an improved simple method for their extraction and identification.
Total ganglioside extracts prepared from brain tissue were concentrated either by dialysis against Carbowax or by employing Millipore filter cones. Thin-layer chromatography was then carried out using silica gel plates. After location of the various fractions quantitation was effected by direct densitometry. The methods that have been adopted are rapid and suitable for the study of brain gangliosides in post mortem and biopsy material in a clinical chemistry laboratory.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002415:Cats; D002855:Chromatography, Thin Layer / Q000379:methods*; D005677:G(M1) Ganglioside / Q000302:isolation & purification; D005678:G(M2) Ganglioside / Q000302:isolation & purification; D005732:Gangliosides / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D005733:Gangliosidoses / Q000378:metabolism; D006801:Humans; D009084:Mucopolysaccharidosis III / Q000378:metabolism; D013661:Tay-Sachs Disease / Q000378:metabolism
D005732:Gangliosides; D005678:G(M2) Ganglioside; D005677:G(M1) Ganglioside