Measurement of enolase molecules in the single preimplantation rat embryo by an enzyme immunoassay system.
The amount of enolase (EC 4.2.1.11) molecules in a single preimplantation rat embryo was determined by an enzyme immunoassay system. An advantage of this assay system is its ability to distinguish each isozyme (alpha alpha, alpha gamma and gamma gamma forms) by using the specific antisera to alpha and gamma subunits. The isozyme of enolase was mostly the alpha alpha form through one-cell to blastocyst stages, whereas the alpha gamma and gamma gamma forms were scarcely detected. Since the immunoassay system is highly sensitive, and minimum amount detectable being 1 amol (1 X 10(-18) mole), the amount of alpha alpha form enolase could be determined in single embryos in all preimplantation stages. The results have revealed that the amount of alpha alpha form enolase molecules does not change through one-cell to morula stages, but increases remarkably between morula and blastocyst stages.
D000818:Animals; D001755:Blastocyst / Q000201:enzymology*; D005260:Female; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000378:metabolism; D046911:Macromolecular Substances; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains
D007106:Immune Sera; D007527:Isoenzymes; D046911:Macromolecular Substances; D010751:Phosphopyruvate Hydratase