Astrocyte and oligodendrocyte distribution in adult rat cerebellum: an immunohistological study.
The distribution of the different types of glial cell in adult rat cerebellar cortex and in the underlying white matter was studied by immunohistology with a specific immune serum raised against form II of carbonic anhydrase, a specific marker for oligodendrocytes in rat cerebellum and an immune serum raised against glial fibrillary acidic protein, a specific astrocyte marker. The cellular specificity of each marker was confirmed by experiments in which the two antigens were revealed in the same cerebellar section. The unequivocal identification, with the optical microscope, of the different glial cell types allowed also a tentative estimation of the number of oligodendrocytes and astrocytes, in relation to Purkinje cells.
D000818:Animals; D001253:Astrocytes / Q000032:analysis / Q000166:cytology*; D002256:Carbonic Anhydrases / Q000032:analysis; D002452:Cell Count; D002525:Cerebellar Cortex / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D009457:Neuroglia / Q000166:cytology*; D009836:Oligodendroglia / Q000166:cytology* / Q000201:enzymology; D011506:Proteins / Q000032:analysis; D011689:Purkinje Cells / Q000166:cytology; D051381:Rats
D011506:Proteins; D002256:Carbonic Anhydrases