Topographical localization of intracellular immunoglobulins in hairy cells by immunoelectron microscopy.
Ultrastructural characterization and localization of intracellular immunoglobulin in peripheral blood mononuclear cells from a patient with hairy cell leukemia were studied by the direct immunoperoxidase technique. Approximately 20--30% of hairy cells stained positive for gamma-heavy and K-light chains, and this staining was limited to certain rough endoplasmic reticulum profiles. These ultrastructural findings can be considered an additional argument for the B cell origin of hairy cells.
D004721:Endoplasmic Reticulum / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007143:Immunoglobulin Heavy Chains / Q000302:isolation & purification*; D007147:Immunoglobulin Light Chains / Q000302:isolation & purification*; D007142:Immunoglobulin gamma-Chains / Q000302:isolation & purification*; D007145:Immunoglobulin kappa-Chains / Q000302:isolation & purification*; D007943:Leukemia, Hairy Cell / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology*; D008854:Microscopy, Electron
D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007142:Immunoglobulin gamma-Chains; D007145:Immunoglobulin kappa-Chains