Reaction of glutaraldehyde with human erytrocytes.
The reaction of glutaraldehyde with human erythrocytes has been studied at pH values of 6.0, 7.2 and 8.0 at several glutaraldehyde concentrations, for different periods at 37 degrees C. Increasing pH and reagent concentration favours formation of cells resistant to osmotic and chemical (by n-butanol, saponin and sodium dodecyl sulfate (SDS)) hemolysis. Ultrasonic waves also have no effect upon these cells. Different behavior concerning the state of hemoglobin, K+ efflux, and swelling have also been observed, according to the pH of treatment. The results suggest that crosslinking involving hemoglobin molecules and components of the membrane (proteins, aminophospholipids) may be obtained in the reaction of human erythrocytes with glutaraldehyde.
D000447:Aldehydes / Q000494:pharmacology*; D003432:Cross-Linking Reagents; D004588:Electrophoresis, Cellulose Acetate; D004912:Erythrocytes / Q000187:drug effects*; D005976:Glutaral / Q000494:pharmacology*; D006461:Hemolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration
D000447:Aldehydes; D003432:Cross-Linking Reagents; D005976:Glutaral