[Selective inhibition of the fluorescence of benzo(a)pyrene and a mutagenic diol-epoxide derivative in living cells by liposoluble iodinated hydrocarbons].
It is shown that in living cells, some liposoluble collisional quenchers like n-iodo hexane and iodo benzene can be used to selectively quench the benzo(a)pyrene fluorescence, leving the diol-epoxide benzo(a)pyrene metabolite emission. The preferential solubilities of these compounds, fluorophors and quenchers, explain the results and the fluorophor location.
D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism*; D004852:Epoxy Compounds / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006847:Hydrocarbons, Iodinated / Q000494:pharmacology*; D066298:In Vitro Techniques; D051379:Mice; D013050:Spectrometry, Fluorescence
D001580:Benzopyrenes; D004852:Epoxy Compounds; D006847:Hydrocarbons, Iodinated
Inhibition sélective dans des cellules vivantes de la fluorescence du benzo(a)pyrène et d'un dérivé epoxyde-diol mutagène par des hydrocarbures iodès liposolubles.