Membrane associated proteins and malignancy: an experimental hypothesis.
Normal human lymphocytes and human tumor cell lines were labeled with an apolar fluorescent probe (1, 6-diphenyl-1, 3, 5-hexatriene), and fluoresence polarization (P) values were determined. The arguments presented in this study suggest that although P values represent an intricate average of all labeled hydrophobic regions of the cell (phospholipid bilayers, membrane proteins and enzymes, etc.), to a large extent, interactions of the probe with membrane proteins are of primary importance. The lower P values obtained with the tumor cell lines, compared with the normal lymphocytes, were interpreted as indicating alterations in either the structure or concentration of a membrane associated protein(s).
D000818:Animals; D000001:Calcimycin; D002462:Cell Membrane / Q000032:analysis; D004161:Diphenylhexatriene; D006801:Humans; D007938:Leukemia / Q000032:analysis; D008214:Lymphocytes / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis*; D051379:Mice; D008808:Mice, Inbred CBA; D009363:Neoplasm Proteins / Q000032:analysis*; D009369:Neoplasms / Q000032:analysis; D013050:Spectrometry, Fluorescence
D008565:Membrane Proteins; D009363:Neoplasm Proteins; D004161:Diphenylhexatriene; D000001:Calcimycin