Immunofluorescent staining of plastic-embedded renal tissue.
Human renal tissues were fixed in cold 4% paraformaldehyde and 0.2% picric acid, embedded in glycol methacrylate (GM), and sectioned at a thickness of 1 to 8 micrograms. Sections were incubated for three hours with nonspecific protease, stained with fluorescein isothiocyanate-conjugated goat antihuman serum samples, and examined by immunofluorescent microscopy. The immunofluorescent staining was compared with that in cryostat sections of the same tissues prepared and stained by conventional methods. The GM-embedded sections showed consistent staining of antigens, comparable with that in the corresponding cryostat sections, and better preservation of tissue structure than in cryostat sections. The antigens in tissues processed by this method are probably stable for long periods.
D000941:Antigens / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006652:Histological Techniques; D006801:Humans; D007668:Kidney / Q000276:immunology*; D010969:Plastics
D000941:Antigens; D010969:Plastics