Dengue-2 vaccine: preparation from a small-plaque virus clone.
The S-1 clone of dengue type 2 virus was used for the preparation of a live-attenuated vaccine after passage in DBS-FRhL-2 cell culture. The vaccine virus had a relatively higher replicative capacity at superoptimal temperatures than its precursor virus, S-1, passaged in primary green monkey kidney cells (S-1 PGMK). There was also a tendency for the S-1 vaccine virus to exhibit leakiness at increased temperatures. Another in vitro marker, replication in monkey peripheral blood leukocytes, indicated less host restriction for the S-1 vaccine in comparative assays with S-1 PGMK virus. Mouse virulence appeared to remain stable on passage in DBS-FRhL-2 cells, whereas monkey immunogenicity decreased. Cautious trials of the dengue type 2 S-1 vaccine in humans are indicated.
D000818:Animals; D002999:Clone Cells; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology*; D000882:Haplorhini; D008253:Macaca mulatta; D051379:Mice; D009154:Mutation; D010641:Phenotype; D013696:Temperature; D014613:Vaccines, Attenuated; D014765:Viral Vaccines / Q000276:immunology / Q000302:isolation & purification*; D014766:Viremia; D014774:Virulence
D014613:Vaccines, Attenuated; D014765:Viral Vaccines