Vitamin E and the inhibition of platelet lipoxygenase.
Lipoxygenase was assayed by the formation of hydroxy-eicosatetraenoic acid (HETE) from arachidonic acid in human and rabbit washed platelets. Platelets from vitamin E-supplemented rabbits had much less lipoxygenase activity than platelets from either vitamin E-deficient or normal rabbits. Human and rabbit platelets preincubated with vitamin E had lowered lipoxygenase activity. These data show that vitamin E inhibits platelet lipoxygenase. Vitamin E and vitamin E acetate, in vitro, were equally effective inhibitors of lipoxygenase. Tween 20, in vitro, was a highly effective inihbitor of lipoxygenase. These data show that vitamin E functions, in vitro, as a surfactant in the inhibition of platelet lipoxygenase.
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D001095:Arachidonic Acids / Q000097:blood / Q000378:metabolism; D001792:Blood Platelets / Q000201:enzymology*; D004032:Diet; D005609:Free Radicals; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D008084:Lipoxygenase / Q000097:blood; D016859:Lipoxygenase Inhibitors; D011817:Rabbits; D013501:Surface-Active Agents; D014810:Vitamin E / Q000494:pharmacology*; D014811:Vitamin E Deficiency / Q000201:enzymology
D001095:Arachidonic Acids; D005609:Free Radicals; D016859:Lipoxygenase Inhibitors; D013501:Surface-Active Agents; D014810:Vitamin E; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D008084:Lipoxygenase; D007213:Indomethacin