[Preparation and comparative evaluation of peroxidase conjugates based on pure antibodies and the IgG Fab fragment].
The results of the preparation of peroxidase conjugates on the basis of the Fab-fragment of rabbit antivaccinal serum IgG and pure antirabbit IgG are presented. Peroxidase conjugates prepared on the basis of highly purified antibodies have been found (in vaccine virus-infected cell cultures) to ensure greater reliability of the immunoperoxidase method due to the decrease of nonspecific reactions registered by the control test system. This allows recommending peroxidase conjugates prepared on the basis of higher purified antibodies for use in diagnostic tests.
D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D007124:Immunoenzyme Techniques; D007140:Immunoglobulin Fab Fragments / Q000276:immunology* / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits
D000906:Antibodies; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G
Poluchenie i sravnitel'naia otsenka peroksidaznykh kon'iugatov na osnove chistykh antitel i Fab-fragmenta IgG.