An automated fluorimetric assay for the simultaneous determinations of histamine and 5-hydroxytryptamine released from rat mast cell preparations.
An automated assay suitable for the extraction and simultaneous fluorimetric determination of histamine and 5-hydroxytryptamine (5-HT) released from rat isolated peritoneal mast cells is described. The method gave good reproducibility and accuracy in use and required a sample volume of 0.5 ml for assay at a sampling rate of 30/h. The system was evaluated by assessment of its performance in the determination of histamine and 5-HT released from rat mast cells in response to compound 48/80. The detection limit for the procedure was 2 ng 5-HT base/ml and 20 ng histamine base/ml.
D000327:Adsorption; D000818:Animals; D001322:Autoanalysis; D004492:Edetic Acid; D006636:Histamine Release; D007158:Immunologic Techniques; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D012701:Serotonin; D003189:p-Methoxy-N-methylphenethylamine; D004492:Edetic Acid