Nerve cells immunoreactive for vasoactive intestinal polypeptide in dissociation cultures of rat hypothalamus and midbrain.
Immunocytochemistry using the unlabeled antibody (PAP) technique reveals the presence of 0.01-0.3% neurons reacting with an antiserum against vasoactive intestinal polypeptide (VIP) in dissociation cultures of hypothalamus and midbrain. The cultures are obtained from 18-day-old rat fetuses and maintained for 22 and 29 days in vitro. Most of the neurons appear well differentiated giving off branched processes with pronounced varicosities. These can often be seen to contact perikarya as well as processes of unstained neurons.
D000818:Animals; D002454:Cell Differentiation; D046508:Culture Techniques; D005260:Female; D005333:Fetus; D005768:Gastrointestinal Hormones / Q000378:metabolism*; D007031:Hypothalamus / Q000166:cytology*; D007124:Immunoenzyme Techniques; D008636:Mesencephalon / Q000166:cytology*; D009474:Neurons / Q000166:cytology; D011247:Pregnancy; D051381:Rats; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
D005768:Gastrointestinal Hormones; D014660:Vasoactive Intestinal Peptide