Detection of staphylococcal enterotoxigenicity. I. Methods.
A culture medium has been developed, using the components easily available in Czechoslovakia, that ensures high-yield production of staphylococcal enterotoxins. Its quality was tested by the "cellophane-over-age" cultivation method using standard prototype strains of staphylococci. The microprecipitation method for the detection of staphylococcal enterotoxins was modified and practically tested. Its sensitivity was 0.3 micrograms/ml in enterotoxins A, B and C, and 0.6 micrograms/ml in types D and E.
D003470:Culture Media; D004768:Enterotoxins / Q000032:analysis / Q000096:biosynthesis*; D005779:Immunodiffusion / Q000379:methods; D013211:Staphylococcus aureus / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D004768:Enterotoxins