Biochemical and allergenic properties of the house dust mite extract, Dermatophagoides pteronyssinus.
A further study has been made on the house dust mite extract, Dermatophagoides pteronyssinus, with emphasis on gel-filtrated fraction 2 (F2). The crude mite extract showed at least nine discs on polyacrylamide disc electrophoresis and contained less than 0.25% sialic acid and less than 0.5 mM hexosamine and no detectable uronic acid. From gel filtration of the crude extract a No 2 fraction (F2) with allergenic activity showed at least five components on SDS disc electrophoresis covering a molecular weight range of between 15,000 and 70,000. The major allergenic activity of F2 dissolved in pH 7-8 and 4-5 on an isoelectric focusing column. Affinity chromatography and lectins showed that allergenic activity did not relate to structures of N-acetyl-D-glucosamine or N-acetyl-D-galactosamine. Allergenic activity of the crude extract was no affected by peptic digestion and the mite digest prepared by trypsin and pronase showed a similar fractionation and activity profile as crude extract.
D000485:Allergens / Q000032:analysis* / Q000276:immunology; D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004391:Dust; D004589:Electrophoresis, Disc; D007158:Immunologic Techniques; D007525:Isoelectric Focusing; D008925:Mites / Q000276:immunology*; D008970:Molecular Weight; D012794:Sialic Acids / Q000032:analysis
D000485:Allergens; D004391:Dust; D012794:Sialic Acids