Feulgen-DNA changes in the germ cells of the male vole (Microtus agrestis) during their development.
Consistent with changes in ploidy during germ cell maturation, the quantity of Feulgen DNA in round spermatids of voles is approximately a quarter of that in primary spermatocytes. The amount increases slightly in elongated spermatids, and then declines in spermatozoa from the testis, epididymis and vas deferens, below the level of round spermatids. Simultaneously nuclear area decreases so that in spermatozoa it is about one tenth that of primary spermatocytes. The rise in Feulgen DNA in elongated spermatids above the level found in round spermatids, and the decline in spermatozoa below this level, is probably caused by altered affinity of the desoxyribose for the leucofuchsin. This may be brought about by the complex physico-chemical changes occurring in chromatin and elsewhere in the nucleus and cell as they acquire their highly specialised final form.
D000818:Animals; D003411:Arvicolinae / Q000033:anatomy & histology* / Q000254:growth & development; D004247:DNA / Q000032:analysis*; D008297:Male; D012741:Sexual Maturation; D013087:Spermatids / Q000032:analysis*; D013090:Spermatocytes / Q000032:analysis*; D013094:Spermatozoa / Q000032:analysis*
D004247:DNA