Modified polynucleotides. VI. Properties of a synthetic DNA containing the anti-herpes agent (E)-5-(2-bromovinyl)-2'-deoxyuridine.
A new modified polydeoxynucleotide, a copolymer of nucleotides of 2'-deoxyadenosine and the very efficacious anti-herpesvirus agent (E)-5-(2-bromovinyl)-2'-deoxyuridine was synthesized with E. coli DNA polymerase I enzyme. It is characterized by its physical (absorption and circular dichroism spectra, thermal transition, sedimentation analysis) and bioorganic (template activity, stability) properties. Compared to poly [d(A-T)], the modified polydeoxynucleotide had a lower thermal stability but exhibited higher stability against DNases and higher template activity for DNA synthesis. Template activity for RNA synthesis of this template was, however, poor and extent of AMP and UMP incorporation was limited as well.
D000998:Antiviral Agents; D001973:Bromodeoxyuridine / Q000031:analogs & derivatives*; D002942:Circular Dichroism; D004247:DNA / Q000138:chemical synthesis*; D004256:DNA Polymerase I / Q000378:metabolism; D004261:DNA Replication; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D013696:Temperature; D013698:Templates, Genetic
D000998:Antiviral Agents; C020235:brivudine; D004247:DNA; D004256:DNA Polymerase I; D001973:Bromodeoxyuridine