[Serodiagnosis of human fasciolasis by micro-ELISA with reference to homologous somatic or excretory-secretory antigens. Comparison with an indirect immunofluorescent technic].
A new type of antigen constituted by excretory-secretory products (ES antigens) of adult Fasciola hepatica cyst alive in vitro is studied by the micro-ELISA method and compared with somatic antigens obtained from adult liver flukes. The results are practically the same (especially the sensitivity and the specificity of the test) but ES antigens seem to have more antigenicity. The results given by the ELISA method and by the IFAT (immunofluorescence antibody test) do not show any connection between the two tests, as the antigens employed are distinct, but IFAT gives a lightly inferior percentage of positivity to that obtained by ELISA.
D000941:Antigens / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005211:Fascioliasis / Q000175:diagnosis* / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques
D000941:Antigens
Séro-diagnostic de la fasciolase humaine par micro-ELISA face à des antigènes homologues somatiques ou excrétés-sécrétés. Comparaison à l'immuno-fluorescence indirecte.