Calcium-activated neutral protease. Its localization in the myofibril, especially at the Z-band.
Calcium-activated neutral protease (CANP) was demonstrated in myofibrils, especially at the Z-band in chicken glycerinated myofibrils, by an immunofluorescent method. The amount of CANP bound to myofibrils was approximately 4 percent of that contained in the whole muscle homogenate. No immunological difference was recognized between the myofibril-bound CANP and the soluble one when examined by Ouchterlony's immunodiffusion analysis. A possible role of myofilbril-bound CANP in the physiological turnover of the myofibrillar proteins is discussed.
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002645:Chickens; D004789:Enzyme Activation; D005455:Fluorescent Antibody Technique; D006801:Humans; D005779:Immunodiffusion; D009210:Myofibrils / Q000201:enzymology* / Q000648:ultrastructure; D010447:Peptide Hydrolases / Q000032:analysis*
D010447:Peptide Hydrolases; D002118:Calcium