PHA selection electrophoresis--a screening method for monomeric IgM.
Pentameric IgM is precipitable by PHA whereas monomeric IgM is not. During electrophoresis in PHA-containing agar gel pentameric IgM is selected by precipitation from concomitant monomeric IgM which may be visualized separately as a precipitation line with specific antibody. This antibody-like use of the lectin in PHA selection electrophoresis provides a quick and simple method of screening macroglobulinemic sera and other biological fluids for the presence of naturally occurring monomeric IgM subunits.
D000042:Absorption; D000818:Animals; D001665:Binding Sites; D003208:Concanavalin A / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D006041:Goats; D006801:Humans; D007122:Immunoelectrophoresis; D007075:Immunoglobulin M; D010265:Paraproteinemias / Q000175:diagnosis*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D011108:Polymers; D011234:Precipitins; D011817:Rabbits; D012276:Ricin / Q000494:pharmacology; D008258:Waldenstrom Macroglobulinemia / Q000175:diagnosis*
D007075:Immunoglobulin M; D010835:Phytohemagglutinins; D011108:Polymers; D011234:Precipitins; D003208:Concanavalin A; D012276:Ricin