[Characteristics of culturing human lymphoid cells].
The growth characteristics of the human lymphoid cells were studied under various cultivation conditions. The cells were grown in flasks with different volumes of nutrient medium, in a spinner or in a specially constructed cultivator providing automatic maintenance of pH and gentle shaking of the suspension. It was found that the average time of the lymphoid cell generation decreases by 2-fold when the cells were grown in the cultivator. Under these conditions, normal growth of cells without lag period starts at a relatively low initial concentration (3.5 X 10(4) cells/ml) as compared with the usual eventual concentration (1.2 X 10(6) cells/ml). The same cells were used for the toxicity testing of some materials used for construction of the cultivation equipment.
D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D003584:Cytological Techniques / Q000295:instrumentation; D006801:Humans; D008214:Lymphocytes / Q000502:physiology*; D013997:Time Factors
D003470:Culture Media
Nekotorye osobennosti kul'tivirovaniia limfoidnykh kletok cheloveka.