Replication of the 2-micrometer DNA plasmid of yeast.
The 2-micrometer DNA plasmid of yeast provides an useful probe for the analysis of the factors involved in the initiation of chromosomal DNA replication in the cell division cycle. Cell-free extracts prepared from growing yeast cells stimulate DNA replication directed by this plasmid. The plasmid replicating activity is subject to control in the yeast cell cycle. The 2-micrometer DNA plasmid can be isolated from logarithmically-growing cells in association with yeast folded chromosomes. The interaction of the plasmid with structures in the cell corresponding to the folded chromosome appears dynamic and cell cycle-dependent. Thus, not only the induction and activity of the proteins involved in replication, but also the intracellular locus of a replicon may be important factors in the control of initiation of DNA replication.
D002453:Cell Cycle; D002501:Centrifugation, Zonal; D002875:Chromosomes / Q000378:metabolism; D004261:DNA Replication; D004270:DNA, Circular / Q000096:biosynthesis*; D004271:DNA, Fungal / Q000096:biosynthesis*; D004274:DNA, Recombinant; D008854:Microscopy, Electron; D009685:Nuclear Envelope / Q000378:metabolism; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013698:Templates, Genetic
D004270:DNA, Circular; D004271:DNA, Fungal; D004274:DNA, Recombinant