[Correlation between the loss of plasmid DNA and the transition from virulent phase I to avirulent phase II in Shigella sonnei].
Transition from virulent phase I to non virulent phase II in five strains of Shigella sonnei was studied by immunofluorescence and plasmid electrophoresis. High frequency loss of phase I specific antigens was observed by use of fluorescent phase specific antibodies. Agarose gel electrophoresis of bacterial lysates showed that transition from phase I to phase II is associated with the loss of extrachromosomal DNA.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D004269:DNA, Bacterial / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D010957:Plasmids; D011817:Rabbits; D012764:Shigella sonnei / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D014774:Virulence
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D004269:DNA, Bacterial
Corrélation entre la perte d'ADN plasmidique et le passage de la phase I virulente à la phase II avirulente chez Shigella sonnei.