[Long-term production and culture of clones of human T-lymphocytes].
Culture of blood T lymphocytes collected from normal individuals and cancer patients were carried out in presence of T cell growth factor (TCGF); these cultures presented cytotoxic activity directed against different targets (lectin activated cells, autologous cancer cells, antibody coated cells and K 562). In order to study separately the different effector subpopulations, isolation of single cultured cells were performed with the help of a micropipette under microscope and monoclonal cultures were carried out in presence of TCGF. In the preliminary cytotoxic assays performed in the clones: (1) a marked activity directed against lectin targets was observed in many clones and (2) an important N K activity was exhibited by the clone 45 B9 (65% of the tested cells lysed human lymphoma K 562 cells).
D002999:Clone Cells / Q000166:cytology*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D013601:T-Lymphocytes / Q000166:cytology* / Q000502:physiology; D013997:Time Factors
Production et culture à long terme de clones de lymphocytes T humains.