Preparation of phosphatidyl[2-3H]inositol from yeast grown in medium containing myo[2-3H]inositol.
Phosphatidyl[2-3H]inositol was prepared from Saccharomyces cerevisiae (YSC-2), grown in synthetic medium containing myo[2-3H]inositol. Over 44 microCi (or 81%) of the radiolabeled inositol was taken up by the organism, with 34 microCi incorporated into phosphatidylinositol. Upon purification by silicic acid pressure liquid chromatography (MPLC), a final yield of 24 to 26 microCi of phosphatidyl[2-3H]inositol with a specific radioactivity of 40 X 10(3) dpm/nmole was obtained. The purified phosphatidyl[2-3H]inositol was found to be a suitable for phospholipase C from human platelets.
D007294:Inositol / Q000378:metabolism; D008722:Methods; D010716:Phosphatidylinositols / Q000302:isolation & purification*; D011849:Radioactive Tracers; D012441:Saccharomyces cerevisiae / Q000032:analysis; D014316:Tritium; D010738:Type C Phospholipases / Q000032:analysis
D010716:Phosphatidylinositols; D011849:Radioactive Tracers; D014316:Tritium; D007294:Inositol; D010738:Type C Phospholipases