Application of a minicolumn detection method for screening spices for aflatoxin.
The minicolumn of Holaday and Lansden was modified by increasing the height of neutral alumina and including a layer of anhydrous sodium sulphate. Using this procedure, aflatoxin was detected in 18 of 125 samples of black pepper, red pepper, ginger and turmeric. A few samples of each spice contained aflatoxin, although red pepper and turmeric showed the highest incidence. Aflatoxin B1 quantities ranged from 10 ug/kg to 120 ug/kg when estimated quantitatively by visual comparison with standards on thin-layer chromatography plates.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000032:analysis*; D002845:Chromatography / Q000379:methods; D002855:Chromatography, Thin Layer; D003212:Condiments / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D013045:Species Specificity
D000348:Aflatoxins; D016604:Aflatoxin B1