Effects of saline exposure on the response of toad bladder (Bufo marinus) to aldosterone.
1. The short-circuit current response of toad bladders to high concentrations of aldosterone (2 x 10(-7) M) has been investigated following saline exposure of toads. 2. Limited saline exposure appears to enhance the aldosterone-stimulated response. 3. Additional saline exposure results in a reduced aldosterone response. 4. Overnight pre-incubation of isolated bladders proved a better pretreatment condition for study of an aldosterone short-circuit current response. 5. Plasma aldosterone concentrations and studies of [3H]aldosterone binding in the bladders have failed to demonstrate significant effects of saline exposure on endogenous aldosterone concentrations.
D000450:Aldosterone / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D002024:Bufo marinus / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004359:Drug Therapy, Combination; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects*; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D013997:Time Factors; D001743:Urinary Bladder / Q000378:metabolism / Q000502:physiology*
D011965:Receptors, Glucocorticoid; D012965:Sodium Chloride; D000450:Aldosterone