Elaboration of both the group W135 and group Y capsular polysccharides by a single strain of Neisseria meningitidis.
A single strain (8021) of Neisseria meningitidis, isolated from a child with disseminated meningococcal disease, was found to elaborate two serogroup-specific capsular polysaccharides-Y and W135. The original isolate as well as the progeny of ten single colony sub-isolates each agglutinated with both group Y and group W135 serogrouping antisera. The capsular polysccharide of strain 8021 contained the chemical constituents of both the W135 and Y capsular polysaccharides in a ratio of about 2.5:1. The patient responded immunologically to both capsular polysaccharides with haemagglutinating antibodies. Analysis by double diffusion in agar revealed that the capsular polysaccharide of strain 8021 contained individual molecules of group W135 and group Y capsular polysaccharides as well as a mosaic molecule containing both antigenic determinants.
D000942:Antigens, Bacterial / Q000032:analysis; D006386:Hemagglutination Tests; D005779:Immunodiffusion; D009345:Neisseria meningitidis / Q000145:classification* / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000378:metabolism*; D012703:Serotyping
D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial