Application of single radial immunodiffusion for qualitative comparison of plant virus particles.
The three fundamental types of precipitin patterns obtained with plant viruses by double radial immunodiffusion (DRID) tests can also be obtained by single radial immunodiffusion (SRID) tests. Using virus antigens that were serologically identical or unrelated, the reaction patterns were readily recognized. However, the precipitin reaction corresponding to the spur in DRID tests using virus antigens that were partially identical serologically was difficult to distinguish. It seems, therefore, that when virus isolates are serologically closely related, DRID should be preferred to SRID. Different viruses in a mixed infection can be successfully distinguished by SRID and also by 'rocket' immunoelectrophoresis.
D000956:Antigens, Viral / Q000032:analysis*; D005779:Immunodiffusion / Q000379:methods; D009029:Mosaic Viruses / Q000276:immunology*; D010942:Plant Viruses / Q000145:classification; D010944:Plants / Q000382:microbiology
D000956:Antigens, Viral