Comparative immunohistochemical localization of IgA and of the IgA part of the bile lipoprotein complex in dog and rat liver.
In bile, phospholipids and cholesterol were found in close association with an apoprotein fraction which cross-reacted to antiserum anti-IgA. In order to determine the origin of the IgA part of the apo bile lipoprotein complex and to establish its relationship with IgA, th localizations of these two components in rat and in dog livers were compared. Two immunohistochemical methods were used. Whereas IgA was found on the sinusoidal surface of the hepatocytes, in cytoplasmic particles and in bile canaliculi, the IgA part of the apo bile lipoprotein complex was only detected in the bile canaliculi, and in particles which presented the same pattern of location as lysosomes. These results suggest that the IgA part of the apo bile lipoprotein complex could originate from the transit of a part of the internalized IgA via the lysosomal compartment of the hepatocytes.
D000818:Animals; D001053:Apolipoproteins / Q000032:analysis*; D001646:Bile / Q000032:analysis*; D004285:Dogs; D006651:Histocytochemistry / Q000379:methods; D006735:Horseradish Peroxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D011817:Rabbits; D051381:Rats
D001053:Apolipoproteins; D007070:Immunoglobulin A; D006735:Horseradish Peroxidase