Loss of effectiveness of preservative systems of mascaras with age.
The preservative systems of unused, anhydrous mascaras were challenged periodically with microorganisms, using a modified membrane procedure. Shelf life of preservative activity against Pseudomonas aeruginosa varied for different brands from as little as 1 month to over 36 months. Generally, P. aeruginosa grew in mascaras after the mascaras were challenged first with Staphylococcus epidermidis or Candida albicans.
D002176:Candida albicans / Q000254:growth & development*; D003358:Cosmetics; D010592:Pharmaceutic Aids; D011310:Preservatives, Pharmaceutical; D011550:Pseudomonas aeruginosa / Q000254:growth & development*; D013210:Staphylococcus / Q000254:growth & development*; D013997:Time Factors
D003358:Cosmetics; D010592:Pharmaceutic Aids; D011310:Preservatives, Pharmaceutical