Separation of peptides and amino acids by ion-exchange chromatography of their copper complexes.
The method of separating peptides and amino acids using their copper (II) complexes has been reexamined by studying model compounds. A marked improvement in the separation was achieved on DEAE Sephadex columns by variation of the ionic strength of the eluting buffers. The use of the method is illustrated by an examination of a protein hydrolysate used in intravenous feeding (Aminosol).
D000596:Amino Acids / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange / Q000379:methods; D003300:Copper; D006801:Humans; D007202:Indicators and Reagents; D009842:Oligopeptides / Q000302:isolation & purification*; D009994:Osmolar Concentration; D010288:Parenteral Nutrition; D011506:Proteins
D000596:Amino Acids; D007202:Indicators and Reagents; D009842:Oligopeptides; D011506:Proteins; D003300:Copper