J chain synthesis in human myeloma cells: light and electron microscopic studies.
The presence of J chain in human immunocytes containing various immunoglobulin classes has already been reported and in this paper we investigated the presence of J chain in human myeloma cells using light and electron microscopy. Myeloma cells were positive for cytoplasmic J chain in thirteen cases: six of fifteen IgG myeloma, two of seven IgA myeloma, two of nine B-J myeloma and three of three primary macroglobulinaemia. None of the four with benign monoclonal gammopathy was positive for J chain. The ultrastructural localization of J chain in IgG myeloma cells was similar to that of gamma chain, i.e. around the nuclear membrane, in the endoplasmic reticulum (ER) and in the Golgi apparatus. The latter finding is compatible with the presence of J chain in the urine of the same patient. We examined the urine from nine patients with J chain-positive cells and secretion of J chain was confirmed in three patients: two with IgG myeloma and one with B-J myeloma. In noe of twelve in whom the immunocytochemical findings were negative for J chain was there evidence of urinary J chain.
D003593:Cytoplasm / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006801:Humans; D007144:Immunoglobulin J-Chains / Q000096:biosynthesis* / Q000652:urine; D008854:Microscopy, Electron; D009101:Multiple Myeloma / Q000276:immunology* / Q000648:ultrastructure / Q000652:urine
D007144:Immunoglobulin J-Chains