Potentiation of insulin and tolbutamide of the effect of indomethacin on carrageenan paw edema in the rat.
The influence of insulin, tolbutamide and buformin on the effect of indomethacin as measured in the carrageenan paw edema assay in Sprague-Dawley rats has been studied. Small doses of these agents, having no influence on edema formation when given by themselves, were tested in combination with indomethacin. Insulin and tolbutamide potentiated the effect of indomethacin in the early phase of carrageenan paw swelling (2 hr), while they did not significantly alter the late phase (5 hr). Buformin was ineffective at any time. Insulin and tolbutamide in the doses applied moderately lowered blood sugar level, whereas buformin did not, thus tolbutamide-induced potentiation may be due to insulin release. No change in blood pressure of rats was observed under treatment. The possible mechanism of the supra-additive effect of insulin and indomethacin on the edema formation induced by carrageenan is discussed.
D000818:Animals; D000893:Anti-Inflammatory Agents; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects; D002351:Carrageenan; D004357:Drug Synergism; D004487:Edema / Q000139:chemically induced; D005260:Female; D005528:Foot / Q000473:pathology; D007213:Indomethacin / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014044:Tolbutamide / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D001786:Blood Glucose; D007328:Insulin; D002351:Carrageenan; D014044:Tolbutamide; D007213:Indomethacin