Localization of T-lymphocyte areas in human lymphoid tissues by fluorochrome-labelled Helix pomatia A haemagglutinin.
Fluorochrome-labelled Helix pomatia A haemagglutinin (HP) stained T-dependent areas in cryostat tissue sections of human lymph node, tonsil, and spleen and thymocytes within thymic medulla but not within thymic cortex. Neuraminidase treatment of the tissue section was a prerequisite for the positive cell staining. The staining was cell-membrane-associated, and the histological pattern was essentially the same as that observed in parallel sections treated with monoclonal antibodies to T cells (OKT3, Leu-1). In addition, blood vessel walls and connective tissue were also stained by HP conjugates. The latter staining was independent of neuraminidase treatment, resistant to alpha-galactosidase treatment and was not blocked by pretreatment with anti-fibronectin antibody. Body types of staining reaction were specific inasmuch as unlabelled HP and the competitive sugar hapten N-acetyl-D-galactosamine blocked the reaction, Frozen, formalin-fixed tissue sections showed the same stainability and reaction pattern as unfixed sections.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006372:Helix, Snails; D006388:Hemagglutinins; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D008221:Lymphoid Tissue / Q000276:immunology / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000276:immunology / Q000378:metabolism; D014066:Palatine Tonsil / Q000276:immunology / Q000378:metabolism; D011309:Preservation, Biological; D011817:Rabbits; D011975:Receptors, Mitogen; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D013950:Thymus Gland / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D006388:Hemagglutinins; D011975:Receptors, Mitogen