[Fluorometric trihydroxyindolic method for determining catecholamines and 3,4-dihydroxyphenylalanine in urine].
Adrenalin, noradrenalin, 3,4-dioxyphenylalanine and dopamine were determined in a urine sample. Optimization of the method was reached as a result of 1) the elution procedure that combines the advantages of both the column and tube varieties; 2) standardization of the eluate and buffer concentrations, enabling necessary oxidation pH to be obtained rapidly; 3) ionic content of the buffers that intensify fluorescence of the substances under test; 4) conditions of preparing and stabilizing luteins; 5) concurret determination of noradrenalin and dopamine.
D002395:Catecholamines / Q000652:urine*; D004295:Dihydroxyphenylalanine / Q000652:urine*; D004298:Dopamine / Q000652:urine; D004837:Epinephrine / Q000652:urine; D006801:Humans; D009638:Norepinephrine / Q000652:urine; D013050:Spectrometry, Fluorescence / Q000379:methods
D002395:Catecholamines; D004295:Dihydroxyphenylalanine; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine
Fliuorimetricheskiĭ trioksindolovyĭ metod opredeleniia katekholaminov i 3,4-dioksifenilalanina v moche.