Carbodiimide-induced protein--RNA crosslinking in mammalian ribosomal subunits.
RNA--protein interactions in the 60 S subunits of rat liver ribosomes were studied using 1-ethyl-3-dimethylaminopropyl carbodiimide (EDC) under conditions which neither changed the sedimentation coefficient of subunits nor the intactness of their rRNA. EDC induced RNA--protein and protein--protein crosslinkings. Proteins crosslinked to 28 S RNA were identified by two-dimensional gel electrophoreses as L17, L19, L23a and L37a, shown to react with 28 S RNA when using a low dose of UV radiation. Attempts have also been made to use EDC for the studies of RNA--protein interactions in 40 S ribosomal subunits.
D002234:Carbodiimides / Q000494:pharmacology*; D004926:Escherichia coli / Q000378:metabolism; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000494:pharmacology*; D008970:Molecular Weight; D012335:RNA, Ribosomal / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
D002234:Carbodiimides; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D005022:Ethyldimethylaminopropyl Carbodiimide