[A simple selective method for determination of DNA-cytosine-methylase in cell homogenates].
The method under discussion is based on varying stability of a glycoside bond of purine and pyrimidine bases with deoxyribose. The DNA methylated with SAM (CH3-3H) and precipitated on GF/C filters was incubated in 0.5 N HCl for 40 min at 60 degrees. Under these conditions 6-methyladenine was released completely, while a significant amount of 5-methylcytosine was preserved.
D044503:5-Methylcytosine; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000032:analysis*; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D008780:Methyltransferases / Q000032:analysis*; D011865:Radioisotope Dilution Technique; D014316:Tritium
D014316:Tritium; D044503:5-Methylcytosine; D003596:Cytosine; D008780:Methyltransferases; D004248:DNA (Cytosine-5-)-Methyltransferases
Prostoĭ izbiratel'nyĭ metod opredeleniia DNK-tsitosin-metilaznoĭ aktivnosti v kletochnykh gomogenatakh.