[Variations of testosterone uptake by gonadotropic cells of the adenohypophysis of the rat].
Testosterone (T) could be detected by immunocytochemistry after cryoultramicrotomy and T is likely bound on a binding site of a high affinity. Castration and adrenalectomy involve a swift disappearance of T in the gonadotropic cells. Synthesis of new binding sites was induced by daily T injection in the castrated and surrenalectomized Rat from 90 days. This synthesis is inhibited by cycloheximide. These data suggest that binding sites are proteins. The captation of T by the gonadotropic cells was diminished similarly at the diminution of T, afterwards, T captation was stabilized at a weak level which became stable for several weeks. It seems that immunocytochemistry permits the study of the variation of T captation by Rat pituitary gonadotropic cells.
D000315:Adrenalectomy; D000818:Animals; D002369:Castration; D003513:Cycloheximide / Q000494:pharmacology; D008297:Male; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D013739:Testosterone / Q000378:metabolism*
D013739:Testosterone; D003513:Cycloheximide
Etude des variations de la capture de la testostérone par les cellules gonadotropes de l'antéhypophyse du rat.