A single isotope method for the measurement of the volume of the mitochondrial inner compartment.
A method is described to measure the volume of the inner mitochondrial compartment using [51Cr]EDTA as an extracompartmental space marker and gravimetry to determine the total H2O content and the weight of the mitochondria.
D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D002860:Chromium Radioisotopes; D004492:Edetic Acid / Q000378:metabolism; D007424:Intracellular Fluid / Q000378:metabolism*; D008722:Methods; D008929:Mitochondria, Heart / Q000378:metabolism*; D011817:Rabbits
D002860:Chromium Radioisotopes; D004492:Edetic Acid