Determination of alpha 2-plasmin inhibitor in body fluids.
A sensitive method was developed to determine alpha 2 PI at very low concentrations. The minimal measurable concentration of alpha 2 PI was 0.5 ng/assay tube. Using this method, the alpha 2 PI in plasma, urine, amniotic fluid and cerebrospinal fluid was estimated. The mean values and standard deviation for plasma were 5.63 +/- 1.34 mg/dl in 25 males, and 5.29 +/- 1.62 mg/dl in 8 females; in the urine, 0.46 +/- 0.41 microgram/dl in 82 male samples, and 0.69 +/- 0.27 microgram/dl in 8 female samples; in amniotic fluid, 4.13 +/- 2.38 micrograms/dl in 16 samples; and in cerebrospinal fluid, 24.4 +/- 6.6 microgram/dl in 9 samples. The alpha 2 PI level in amniotic fluid was not correlated with the state of the pregnant mother, with that of the baby, or with the protein content or pH of the amniotic fluid.
D000653:Amniotic Fluid / Q000032:analysis; D000818:Animals; D001826:Body Fluids / Q000032:analysis*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D010949:Plasma / Q000032:analysis; D011247:Pregnancy; D011817:Rabbits; D000979:alpha-2-Antiplasmin / Q000032:analysis* / Q000134:cerebrospinal fluid / Q000652:urine
D000979:alpha-2-Antiplasmin