Chemokinetic factors obtained from the larval stage of the cestode, Taenia taeniaeformis.
Saline extracts of the metacestodes of Taenia taeniaeformis were shown to have chemokinetic as well as chemotactic activity for equine polymorphonuclear leucocytes. Although parasite derived chemotactic activity could be appreciated at high protein inputs, such leucotactic activity was quickly lost upon dilution. In contrast chemokinetic activity was readily observed in saline extracts over a much broader range of protein inputs. Utilizing the Zigmond-Hirsch checkerboard assay the chemokinetic properties in the saline extracts were more pronounced when chemokinetic factors were loaded into the cell compartment of the chemotactic chambers only. Mild heat treatment or storage at 4 degrees C resulted in little destruction of the chemokinetic factors. However six cycles of rapid freezing and thawing generated marked increases in chemokinetic activity and not chemotactic activity. The results of this work provide for the first time evidence of parasite derived chemokinetic factors.
D000818:Animals; D002465:Cell Movement; D002630:Chemotactic Factors / Q000032:analysis; D005615:Freezing; D006736:Horses / Q000097:blood; D006863:Hydrogen-Ion Concentration; D016209:Interleukin-8; D009504:Neutrophils / Q000502:physiology; D013621:Taenia / Q000032:analysis* / Q000254:growth & development; D013696:Temperature
D002630:Chemotactic Factors; D016209:Interleukin-8