The effect of serum from obese and normal weight men on glucose metabolism in leucocytes.
The influence of pooled serum from either obese or normal weight males on glucose metabolism in human leucocytes has been studied. Leucocytes from normal weight males were incubated with 10-90% pooled serum and either [U-14C], or [1-14C]glucose. Compared to serum from the normal weight males, serum from the obese group had a more stimulating effect on the 14CO2 and [14C]lactate production from [U-14C]glucose and on the 14CO2 production from [1-14C]glucose. The two serum pools had the same stimulating effect on the Embden-Meyerhof pathway as indicated by the formation of [14C]lactate from [1-14C]glucose. Calculations revealed that the activity in the pentose phosphate pathway was stimulated more by serum from obese, than from normal weight males. It is a possibility that increased stimulation of the pentose phosphate pathway may contribute to the development of overweight.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001786:Blood Glucose / Q000378:metabolism*; D002245:Carbon Dioxide / Q000096:biosynthesis; D006801:Humans; D007773:Lactates / Q000096:biosynthesis; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood*
D001786:Blood Glucose; D007773:Lactates; D002245:Carbon Dioxide