Separation and purification of liver microsomal monooxygenases from induced and untreated pigs.
From liver microsomes of phenobarbital-, beta-naphthoflavone- and ethanol-pretreated male pigs and of controls NADPH-cytochrome P-450 reductase and four cytochrome P-450 fractions were isolated according to a modified procedure of Imai and Sato. Electrophoretic, immunological, spectral and catalytic properties suggested that at least five different forms of cytochrome P-450 could be separated, two of which were obtained in a pure state. Using selective inhibitors and antisera it was concluded that microsomes from phenobarbital-pretreated pigs contain at least three forms which all were metyrapone-sensitive. beta-Naphthoflavone induced probably two 7,8-benzoflavone-sensitive forms. After ethanol administration the main form of cytochrome P-450 was identical to that of controls.
D000818:Animals; D001571:Benzoflavones / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D000431:Ethanol / Q000494:pharmacology; D005779:Immunodiffusion; D008297:Male; D008797:Metyrapone / Q000494:pharmacology; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D010634:Phenobarbital / Q000494:pharmacology; D013057:Spectrum Analysis; D013552:Swine; D019324:beta-Naphthoflavone
D001571:Benzoflavones; D000431:Ethanol; D019324:beta-Naphthoflavone; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital; D008797:Metyrapone