[Research on HBsAg: comparison between two methods of the third generation, RPHA and IEA].
Two tests of the 3rd generation were evaluated for the identification of HBsAg: the reverse hemoagglutination and the enzymatic immunoassay. The results of the study, carried out on 2,434 sera of occasional and periodic donors, have been slightly discordant. It was confirmed the greater sensibility of the IEA test over the RHA and there was a high incidence of false positive results of the IEA test probably caused by the presence in the sera of an elevated concentration of lipid and/or Hb. However, we can consider the RHA a useful test adaptable as an emergency test while the IEA method, being more sensible, can be used as a basic screening test.
D005189:False Positive Reactions; D006386:Hemagglutination Tests / Q000379:methods*; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques
D006514:Hepatitis B Surface Antigens
Ricerca dell'HBsAg: confronto tra due tecniche di terza generazione, RPHA ed IEA.