[Cell culture preservation by automatic freezing in the adapted Höppler ultrathermostat of the NBE type].
A description is presented of an adapted ultrathermostat, respecting the requirement for a continuous decrease in temperature by 1 degree C per minute, as necessary for keeping the frozen cultures alive. Viability was evaluated in the primary cells of calf kidneys in the first subpassage and permanent cell lines PK and TL 72 after their freezing and preservation at -192 degrees C for 30 days. In comparison with regularly passaged cultures, the frozen cells had a high viability.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D005615:Freezing; D007668:Kidney; D014021:Tissue Preservation / Q000295:instrumentation / Q000379:methods*
Konzervace bunĕcných kultur automatickým mrazením na upraveném ultratermostatu Höppler typu NBE.