[Increase in the sensitivity and a simplification of the method for the separate determination of antibiotic activities in combined preparations (oletetrin) by using media with a varying pH value].
An agar-diffusion method for determination of oleandomycin and tetracycline low levels in solutions of the drug combination was developed. The medhod may be used for investigation of oletetrin absorption and distribution in humans and animals. It provides high accuracy in separate determination of oleandomycin and tetracycline activity in solutions of the drugs at a ratio of 1 : 2. The same test-culture, Bac. subtilis, variant L2 is used for the assay of tetracycline and oleandomycin activity. The only differences are in the values of pH and the buffer solution and the standards.
D001412:Bacillus subtilis / Q000187:drug effects; D003470:Culture Media; D004338:Drug Combinations; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009827:Oleandomycin / Q000032:analysis*; D013752:Tetracycline / Q000032:analysis*
D003470:Culture Media; D004338:Drug Combinations; D013752:Tetracycline; D009827:Oleandomycin
Povyshenie chuvstvitel'nosti i urposhchenie metoda razdel'nogo opredeleniia aktivnosti antibiotikov v kombinirovannykh preparatakh (oletetrin) prispol'zovanii sred s razlichnym znacheniem pH.