Chloroplast biogenesis. XXIX. The occurrence of several novel chlorophyll a and b chromophores in higher plants.
With the use of low temperature spectrofluorometry and matrix calculations it was demonstrated that the chlorophyll a pool of higher plants is made up of four different chlorophyll a chromophores. The latter were segregated by high pressure liquid chromatography on a silica column. They were designated Chl a (E432 F664), Chl a (E436 F670), Chl a (E443 F672) and Chl a (E446 F674), where E refers to the Soret excitation maximum and F to the fluorescence emission maximum at 77 K in ether. Likewise the Chl b pool was shown to consist of at least four different Chl b chromophores which were designated: Chl b (E465), Chl b (E470), Chl b (E475) and Chl b (E485). It was proposed that the various chlorophyll chromophores differed by the degree of oxidation of their side chains at the 2 and 4 positions of the macrocycle. It was also suggested that the chemical modifications at the 2 and 4 positions of the macrocycle may play an important role in positioning the different chlorophyll chromophores in the thylakoid membranes.
D002734:Chlorophyll / Q000032:analysis*; D002736:Chloroplasts / Q000502:physiology*; D005056:Euglena gracilis / Q000032:analysis; D010860:Pigments, Biological / Q000032:analysis*; D018521:Plant Physiological Phenomena; D013045:Species Specificity; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
D010860:Pigments, Biological; D002734:Chlorophyll