Genetic control of a shared idiotype among antibodies directed to distinct specificities.
We developed an idiotypic radioimmunoassay system that detects shared idiotypic determinants, termed GTGL idiotype, on antibodies bearing distinct antigen-binding specificities in various mouse strains. Either poly-(Glu, Tyr) (GT)- or poly-(Glu, Lys) (GL)-related determinants are able to induce anti-GT and anti-GL (GTGL)-idiotypic antibodies. Strain distribution studies indicate that GTGL-idiotypic antibodies are readily induced and frequently expressed in antisera obtained from 25 different mouse strains immunized either with GT-related or GL-related polymers. The ability to express GTGL-idiotypic antibodies is a dominant trait and is controlled by Igh-linked gene(s). In addition, we demonstrated that in anticopolymer of L-glutamine acid60- L-alanine30-L-tyrosine (GAT) and anticopolymer of L-glutamic acid54-L-lysine35-L-phenylalanine11(GLphi) antisera, both antibodies uniquely specific to GAT or GLphi, respectively, and antibodies bearing dual specificities for GAT and GLphi, expressed GTGL idiotype. The genetic implications of these findings are discussed.                                                                                 X
D000409:Alanine / Q000276:immunology; D000818:Animals; D000916:Antibody Diversity; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D005796:Genes; D005971:Glutamates / Q000276:immunology; D007106:Immune Sera; D007130:Immunoglobulin Idiotypes / Q000235:genetics*; D008239:Lysine / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D009154:Mutation; D010455:Peptides / Q000276:immunology; D010649:Phenylalanine / Q000276:immunology; D011392:Proline / Q000276:immunology; D014443:Tyrosine / Q000276:immunology
D005971:Glutamates; D007106:Immune Sera; D007130:Immunoglobulin Idiotypes; D010455:Peptides; D014443:Tyrosine; D010649:Phenylalanine; D011392:Proline; D008239:Lysine; D000409:Alanine