Detection of exoerythrocytic stages of Plasmodium berghei in fixed liver tissue and cultured cells by an immunoperoxidase antibody technique.
An immunoperoxidase antibody (IPA) technique is described for the detection of exoerythrocytic (EE) stages of Plasmodium berghei in rat liver sections or cultured W138 cells. EE stages were detected in liver sections 12 hours after inoculation of sporozoites, but as early as three hours after the inoculation of W138 cells. By 42 hours in liver sections and 48 hours in W138 cells, EE parasites were easily visible by low power microscopy, and merozoites were clearly seen by 72 hours within EE parasites in W138 cells, or extracellularly on adjacent cells.
D000818:Animals; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D006801:Humans; D007124:Immunoenzyme Techniques; D008099:Liver / Q000469:parasitology*; D008168:Lung; D010962:Plasmodium berghei / Q000254:growth & development / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains