Requirement of a partially diploid donor for the chemical induction of mutations in transforming DNA.
In the Bacillus subtilis transformation system, in vitro chemical mutagenesis may depend upon the use of a DNA donor carrying two copies of the region of interest. Transformations involving this region of the donor DNA which has been chemically treated may lead to the recovery of new mutations or segregant products by some as yet undetermined mechanism.
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D001412:Bacillus subtilis / Q000235:genetics*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics*; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009153:Mutagens; D009154:Mutation; D011796:Quinacrine / Q000494:pharmacology; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D015112:4-Nitroquinoline-1-oxide; D005020:Ethyl Methanesulfonate; D011796:Quinacrine