[New technic for detecting anti-double-stranded DNA antibodies. Value in disseminated lupus erythematosus in children].
Two assays for antibodies to double stranded (ds) DNA were compared in children with systemic lupus erythematosus (SLE). An indirect immunofluorescent test using Crithidia luciliae (IF-CL) was more specific but slightly less sensitive than the DNA binding assay (RIA). Detection of antibodies to ds-DNA (anti-ds-DNA) by IF on 3 different trypanosoma (T. brusei, T. equiperdum, and T. musculi) was as specific and as sensitive as IF-CL, but more difficult to read microscopically. No correlation was found between immunoglobulin (Ig) class of circulating anti-ds-DNA and severity of renal disease in SLE. A comparison of serologic and immunohistopathologic (kidney and skin) data in 12 children with SLE showed that immune-complex glomerulonephritis was more frequently encountered when anti-ds-DNA was detected in serum, and that Ig deposits were found only in the skin when anti-ds-DNA could not be detected in serum.
D000974:Antibodies, Antinuclear / Q000032:analysis*; D002648:Child; D003421:Crithidia / Q000276:immunology; D004247:DNA / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007668:Kidney / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008722:Methods; D011863:Radioimmunoassay; D012867:Skin / Q000276:immunology; D014345:Trypanosoma / Q000276:immunology
D000974:Antibodies, Antinuclear; D004247:DNA
Technique nouvelle de détection des anticorps anti-ADNn. Intérêt dans le lupus érythémateux disséminé de l'enfant.