Acute lymphoblastic leukaemia cells in the diffusion chamber: change of surface phenotype during the culture period.
Peripheral blast cells from patients with acute lymphoblastic leukaemia were cultured in the diffusion chamber system. On different harvesting days the cALL antigen, surface immunoglobulin and T-cell antigen were investigated by direct immunofluorescence. Each cell type investigated showed a different response throughout culture, the commonest change observed being development into the B-cell axis. Our data demonstrate that the differentiation stop of a leukaemic cell can be overcome by using the diffusion chamber system. The data therefore give some insight into the stage of developmental arrest in acute lymphoblastic leukaemia.
D000951:Antigens, Neoplasm / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology; D002454:Cell Differentiation; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006801:Humans; D007945:Leukemia, Lymphoid / Q000276:immunology*; D010641:Phenotype; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
D000951:Antigens, Neoplasm; D011947:Receptors, Antigen, B-Cell