The use of an excretory-secretory antigen for an ELISA specific sero-diagnosis of visceral larva migrans.
In sera from patients with visceral larva migrans (VLM) syndrome, enzyme-linked immunospecific assay (ELISA) was used to detect IgG and IgE antibody anti-excretory-secretory antigen (ESA) from the second larval stage of Toxocara canis. The technical conditions of the assay were determined. The specificity of IgG ELISA-ESA (with OD values greater than 0.34) allowed the differentiation of VLM syndrome from ascaris or other human parasite infections.
D000906:Antibodies / Q000032:analysis; D000941:Antigens / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000032:analysis; D007816:Larva Migrans, Visceral / Q000175:diagnosis*; D012698:Serologic Tests / Q000379:methods; D014119:Toxocara / Q000276:immunology
D000906:Antibodies; D000941:Antigens; D007073:Immunoglobulin E