Pre-prolactin accumulates in rat pituitary cells incubated with a threonine analog.
The proteolytic processing of rat pre-prolactin (Pre-Prl) to prolactin in isolated hemipituitaries was inhibited by the threonine analog, beta-hydroxynorvaline (Hnv). Pre-Prl accumulated as a major labeled intracellular product, even during pulse labeling longer than 2 h, but it was not secreted. Inhibition of Pre-Prl processing may result from incorporation of Hnv at the Pre-Prl cleavage site. This system offers the opportunity to study the disposition of an accumulated pre-protein in cells.
D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D007774:Lactation; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism*; D011247:Pregnancy; D011388:Prolactin / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis*; D051381:Rats; D013912:Threonine / Q000031:analogs & derivatives* / Q000494:pharmacology
D011498:Protein Precursors; C010994:3-hydroxynorvaline; D013912:Threonine; C017671:preprolactin; D011388:Prolactin