Sex preselection in mammals? Separation of sperm bearing Y and "O" chromosomes in the vole Microtus oregoni.
The two sex determining sperm populations of the vole Microtus oregoni were separated according to DNA content by use of flow sorting instrumentation. Although the sperm were not viable, they should be useful for addressing the question of haploid expression of genes linked to sex chromosomes and for efficiently searching for biochemical markers that differentiate the two populations.
D000818:Animals; D003411:Arvicolinae / Q000235:genetics; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes; D008297:Male; D012730:Sex Chromosomes / Q000648:ultrastructure; D012732:Sex Determination Analysis; D013094:Spermatozoa / Q000502:physiology*
D005456:Fluorescent Dyes; D004247:DNA