A semi-quantitative micromethod for the determination of free glycosaminoglycans in serum. Results from studies on serum of healthy children of various age and patients affected by mucopolysaccharidosis.
A method is presented for the determination of free glycosaminoglycan (GAG) concentration in as little as 30 microliter serum. By filtration of the serum through DEAE-cellulose paper, the free GAG fraction is selectively adsorbed and concentrated on a circular area of 15.9 mm2; these GAG spots are stained with Alcian Blue. The relationship between the amounts of adsorbed GAG and the optical density of the Alcian Blue spots is linear with a certain range; e.g. for chondroitin sulfate (mixed isomers), from 0.25 to 1.00 micrograms. With this method--which we will refer to as the "DEAE Alcian Blue" method--we estimated the free GAG concentration in sera of individuals of various ages including newborns.
D000293:Adolescent; D000328:Adult; D000375:Aging; D000423:Alcian Blue; D002648:Child; D002675:Child, Preschool; D005260:Female; D006025:Glycosaminoglycans / Q000097:blood*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007234:Infant, Premature; D008297:Male; D008832:Microchemistry; D008875:Middle Aged; D009083:Mucopolysaccharidoses / Q000097:blood*; D008059:Mucopolysaccharidosis I / Q000097:blood; D009084:Mucopolysaccharidosis III / Q000097:blood; D013053:Spectrophotometry / Q000379:methods
D006025:Glycosaminoglycans; D000423:Alcian Blue