[Plasmids as cloning vehicles (author's transl)].
Plasmids are regulated autonomously replicating DNA elements, which have been used successfully for the in vitro insertion and the transfer and stable maintenance of defined foreign DNA fragments into bacteria and lower eukaryontes. For Escherichia coli a large set of ColE1 derivatives have been developed and have been used for cloning of DNA fragments from many biological systems. More recently plasmids have been also isolated and modified for the use as cloning vehicle for other bacteria like Bacillus subtilis, Pseudomonas and Streptomyces and for the yeast Saccharomyces cerevisiae.
D001412:Bacillus subtilis / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics; D005057:Eukaryotic Cells / Q000378:metabolism; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics; D013302:Streptomyces / Q000235:genetics
Plasmide als Kloniervehikel.