Effects of indomethacin, acetazolamide, ethacrynate sodium, and atropine on intestinal secretion mediated by Escherichia coli heat-stable enterotoxin in pig jejunum.
Intraluminal perfusion of pig jejunum with Escherichia coli heat-stable enterotoxin reversed net absorption of water and electrolytes to net secretion. Addition of atropine (2 x 10(-5)M) to the perfusate reduced the secretory response to enterotoxin and enhanced sodium and chloride absorption in control segments. Indomethacin (1.4 x 10(-3)M), acetazolamide (2.2 x 10(-3)M), or ethacrynate sodium (3.1 x 10(-4)M) had no effect. Mucosal disaccharidase activity and Na-K-ATPase activity were not altered by enterotoxin. The results suggest that blockade of cholinergically mediated secretion in the small intestine attenuates the enterosorptive effects of heat-stable enterotoxin and may be useful therapeutically in the management of secretory diarrhea.
D000086:Acetazolamide / Q000494:pharmacology*; D000818:Animals; D001285:Atropine / Q000494:pharmacology*; D003967:Diarrhea / Q000139:chemically induced / Q000503:physiopathology*; D004768:Enterotoxins / Q000494:pharmacology*; D004926:Escherichia coli; D004976:Ethacrynic Acid / Q000494:pharmacology*; D007213:Indomethacin / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D007583:Jejunum / Q000502:physiology; D013552:Swine
D004768:Enterotoxins; D001285:Atropine; D004976:Ethacrynic Acid; D000086:Acetazolamide; D007213:Indomethacin