Characterization of beta-cell specific function of isolated pancreatic islets from hyperglycemic C57BL/KsJ-db/db mice.
Isolated pancreatic islets from male hyperglycemic C57BL/KsJ-db/db mice were used to study (pro)insulin biosynthesis, insulin release and insulin content. A diminution of the insulin content to about 36% and of the insulin release (20 mmol/l glucose) to nearly one half was observed, whereas [3H]leucine incorporation into (pro)insulin was increased. Insulin biosynthesis as well as insulin release showed no further increase, when the glucose concentration was increased to 20 or 30 mmol/l. Cultivation of such islets in TCM 199 (10 mmol/l glucose or additionally increased [Mg++] to 5.3 mmol/l) for 48 h resulted in a significant increase of the insulin content and a restoration of the glucose dependent insulin release.
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002478:Cells, Cultured; D006943:Hyperglycemia / Q000378:metabolism*; D006946:Hyperinsulinism / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism*; D007930:Leucine / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D011384:Proinsulin / Q000096:biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis
D001786:Blood Glucose; D007328:Insulin; D011498:Protein Precursors; C013737:preproinsulin; D011384:Proinsulin; D007930:Leucine