[Demonstration of Leptospirae antibodies in the sera of immunized rabbits by the indirect immunofluorescence test (author's transl)].
Antibodies of Leptospirae were demonstrated in the sera of immunized rabbits by the indirect immunofluorescence test, only after a suitable dilution of the "anti-rabbit-gamma-globulin fluorescin conjugated" was carried out in a cross reference titration ("chessboard method"). A distinct crossreaction was observed when anti-icterohaemorrhagiae - and anti-bratislava-immunsera reacted with several serotypes out of the "interrogans complex". But only a weak crossreaction was found when both sera reacted with the serotyp patoc from the "biflexa complex". The evaluation of the indirect immunofluorescence test was complicated by a streaky background of fluorescence. The agargeldiffusion tests (Ouchterolony) exhibited four, three and two precipitation bands according to the conbination of antigens and immunsera.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D007114:Immunization; D005779:Immunodiffusion; D007919:Leptospira / Q000276:immunology*; D011817:Rabbits; D013045:Species Specificity
D000907:Antibodies, Bacterial
Nachweis von Leptospiren-Antikörpern in Seren immunisierter Kaninchen mit Hilfe des indirekten Immunfluoreszenztestes.