The determination by radiochemical assay of argininosuccinase produced in an Escherichia coli system in vitro.
A highly sensitive radiochemical assay used to measure the synthesis and regulation of the product of the argH gene, argininosuccinase, in an Escherichia coli system in vitro is described. With L-[guanidino-14C]argininosuccinic acid as a substrate, and in the presence of excess arginase and urease, 14CO2 is collected in a simply designed micro-vessel. With this method less than 1 nmol of product can be measured in the presence of various concentrations of L-arginine.
D001123:Argininosuccinate Lyase / Q000096:biosynthesis*; D001125:Argininosuccinic Acid / Q000378:metabolism; D002250:Carbon Radioisotopes; D004926:Escherichia coli / Q000201:enzymology*; D008190:Lyases / Q000096:biosynthesis*; D011854:Radiochemistry / Q000379:methods*
D002250:Carbon Radioisotopes; D001125:Argininosuccinic Acid; D008190:Lyases; D001123:Argininosuccinate Lyase