[Measurement of leukocyte accumulation in the capillaries in the lesser circulation of the rat: extraction of cells and characterization of enrichment in monocytes].
We have performed a qualitative and quantitative study of leucocytes in lung alveolar capillaries. This population is not an artefact induced by experimental procedure because the same population is observed after instant cardiac arrest. About 30% of these leucocytes can be extracted by perfusion, the extracted population being representative of the whole one present in capillaries. The experimental procedure used allow to collect 100.10(6) leucocytes per animal 15.10(6) of which are monocytes easily obtained as a purified population. From these results lung capillaries contain at least as many leucocytes as the whole circulating blood and 5 times more monocytes.
D000818:Animals; D002196:Capillaries / Q000166:cytology; D003584:Cytological Techniques; D007962:Leukocytes / Q000502:physiology*; D008297:Male; D009000:Monocytes / Q000502:physiology*; D011650:Pulmonary Alveoli / Q000098:blood supply*; D051381:Rats; D011919:Rats, Inbred Strains
Mesure de l'accumulation leucocytaire dans deoesles capillaires de la petite circulation du rat: extraction des cellules et caractérisation de l'enrichissement deen monocytes.