A semi-automated microassay method for the measurement of ablastin, a division inhibitory antibody.
Division of Trypanosoma lewisi in the rat is terminated by ablastin, a factor(s) in the serum of rats acquired during infection which is believed to be specific antibody. In this study a semi-automated microassay technique for the measurement of ablastin is described. The system involved the use of [3H]TdR as a marker for trypanosome division and a multiple-well cell harvester to harvest trypanosomes from wells of microtitre plates. The method is versatile, quantitative and easy to perform, permitting the simultaneous measurement of numerous samples for this division-inhibitory activity.
D000372:Agglutination Tests; D000818:Animals; D005260:Female; D007074:Immunoglobulin G / Q000032:analysis*; D007158:Immunologic Techniques; D051381:Rats; D011919:Rats, Inbred Strains; D014344:Trypanocidal Agents / Q000032:analysis*; D014350:Trypanosoma lewisi / Q000254:growth & development / Q000276:immunology; D014352:Trypanosomiasis / Q000276:immunology
D007074:Immunoglobulin G; D014344:Trypanocidal Agents; C019230:ablastin