A new approach for the immunogenic presentation of membrane-bound human colon tumor antigens.
Liposomes bearing human tumor membrane vesicles were effective immunogenic complexes for inducing antibodies in rabbits. Multilamellar liposomes (MLV) at 7:4:1 molar ratios of phosphatidylcholine, cholesterol and phosphatidic acid were prepared with sonicated membrane (MN) isolated from LS174T colon tumor cells. MLV liposomes prepared together with MN (i.e., MN-MLV antigens) or MN added to preformed MLV (i.e., MN+MLV antigens), and MN antigens alone were used as immunogens. Rabbits were immunized i.v. with 100 g protein of each antigen group. Boosters (i.v.) were at days 13 and 29. Binding assays were performed by indirect radioimmunoassay on viable tumor cell targets. The MN+MLV groups were distinguished by earlier reactivity and greater specificity to the colon tumor antigens.
D000230:Adenocarcinoma / Q000276:immunology*; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology*; D001666:Binding Sites, Antibody; D003110:Colonic Neoplasms / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera / Q000032:analysis; D008081:Liposomes / Q000276:immunology; D008297:Male; D011817:Rabbits
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D007106:Immune Sera; D008081:Liposomes