Cytogenetic analysis of an immunogenic mutant of the L5178Y lymphoma.
A mutant of the uniformly lethal L5178Y lymphoma, called the L5178Y/Manitoba (L5178Y/M), was rejected after subcutaneous challenge in syngeneic DBA/2 mice. Karyotypic analysis revealed that the parent L5178Y lymphoma had four chromosome markers, with the mutant L5178Y/M sharing one of them as well as possessing two distinguishing markers. One diploid and two hypotetraploid clones were isolated from the L5178Y/M; they contained all the marker chromosomes and were also rejected by the syngeneic host. In addition to the shared chromosome markers, the L5178Y/M possessed antigens in common with the parent L5278Y. DBA/2 mice made immune to the mutant by subcutaneous immunization were able to slow the growth of the parent tumor but not the unrelated P-815-X2 mastocytoma.
D000818:Animals; D002999:Clone Cells; D003429:Cross Reactions; D005260:Female; D005819:Genetic Markers; D006084:Graft Rejection; D007621:Karyotyping; D007940:Leukemia L5178 / Q000235:genetics* / Q000276:immunology; D007942:Leukemia, Experimental / Q000235:genetics*; D008223:Lymphoma / Q000276:immunology; D008297:Male; D012515:Mast-Cell Sarcoma / Q000276:immunology; D051379:Mice; D008811:Mice, Inbred DBA; D009154:Mutation; D014184:Transplantation, Homologous
D005819:Genetic Markers