Replication fork rate and origin activation during the S phase of Saccharomyces cerevisiae.
When the growth rate of the yeast Saccharomyces cerevisiae is limited with various nitrogen sources, the duration of the S phase is proportional to cell cycle length over a fourfold range of growth rates (C.J. Rivin and W. L. Fangman, 1980, J. Cell Biol. 85:96-107). Molecular parameters of the S phases of these cells were examined by DNA fiber autoradiography. Changes in replication fork rate account completely for the changes in S-phase duration. No changes in origin-to-origin distances were detected. In addition, it was found that while most adjacent replication origins are activated within a few minutes of each other, new activations occur throughout the S phase.
D000641:Ammonia / Q000378:metabolism; D002453:Cell Cycle; D004261:DNA Replication; D004271:DNA, Fungal / Q000096:biosynthesis*; D005973:Glutamine / Q000378:metabolism; D007399:Interphase; D007700:Kinetics; D011392:Proline / Q000378:metabolism; D012093:Replicon; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism; D013912:Threonine / Q000378:metabolism
D004271:DNA, Fungal; D005973:Glutamine; D013912:Threonine; D000641:Ammonia; D011392:Proline