[Autoradiographic study of the rate of DNA synthesis in the duct epithelial cells of the epididymis and of the brain subependymal zone in the rat following whole-body x-ray irradiation].
The experimental data have been analyzed on the labeled cell distribution related to the grain count over the nucleus in autographs of histological sections (5 mcm) made of the rat brain subependymal zone and of epididymis duct epithelium at different time after 3H-thymidine injection and X-irradiation in the dose of 300 cGr. These results served some additional grounds to the recently established conclusion that a repeated successive decrease in the rate of DNA synthesis is occurring in the cell system starting from stem cell (level I) to semistem cell (levels II-VII) (Gracheva, 1982 r). 5 days after irradiation, at the peak of reparative proliferation, the cell reproduction was intensified, these cells having normally both middle and high levels of DNA synthesis. This process is running of the background of the inhibition of reproduction of cells with the inherent low level of DNA synthesis which are to start differentiation after mitosis. All this makes for the increase in the mean grain count over the nuclei, without changes of the inherent rates of DNA synthesis in the successive generations of the stem cells.
D000818:Animals; D001345:Autoradiography; D002454:Cell Differentiation / Q000528:radiation effects; D002455:Cell Division / Q000528:radiation effects; D004247:DNA / Q000096:biosynthesis / Q000528:radiation effects*; D004805:Ependyma / Q000378:metabolism / Q000528:radiation effects*; D004822:Epididymis / Q000378:metabolism / Q000528:radiation effects*; D004848:Epithelium / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014916:Whole-Body Irradiation
D004247:DNA
Avtoradiograficheskoe izuchenie skorosti sinteza DNK v kletkakh épiteliia protoka épendidimisa i subépendimnoĭ zony golovnogo mozga krys posle obshchego rentgenovskogo oblucheniia.