Regulation of heart triglyceride synthesis in diabetes.
Heart triglyceride content and triglyceride synthesis were studied in control, ketotic diabetic, and insulin-treated rats. Heart triglyceride content was increased in the ketotic diabetic state but was reversed to control values on insulin treatment of the diabetic rats. Triglyceride synthesis in heart homogenates was sensitive to incubation temperature. Triglyceride synthesis from [U-14C]sn-glycero-3-phosphate was increased in the diabetic state, suggesting that increased synthesis played a role in heart triglyceride accumulation. The addition of the end product of triglyceride synthesis, e.g., triolein, to incubation mixtures resulted in inhibition of triglyceride synthesis in heart homogenates of control and diabetic rats. The results suggested that the enzymes of heart triglyceride synthesis were less sensitive to feedback inhibition in the diabetic state.
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D016883:Diabetic Ketoacidosis / Q000188:drug therapy / Q000378:metabolism; D007328:Insulin / Q000627:therapeutic use; D008297:Male; D008660:Metabolism / Q000187:drug effects; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D013696:Temperature; D014280:Triglycerides / Q000096:biosynthesis*; D014304:Triolein / Q000494:pharmacology
D007328:Insulin; D014280:Triglycerides; D014304:Triolein