Free and membrane-bound ribosomes and NADPH-cytochrome C reductase activity in the liver cells of protein-fed and protein-deprived rats.
Rats were starved for 7 days or fed a protein-free or a 25% casein diet for 7 or 14 days. Free and total ribosomes were prepared from the liver. A determination of the ratio of mg RNA of ribosomes to mg DNA of the total homogenate was made. In starved rats, RNA of free and membrane-bound ribosomes decreased to the same extent. In rats fed a protein-free diet only mg RNA of membrane-bound ribosomes decreased significantly, and after 14 days the ratio of RNA of free to total ribosomes was increased. NADPH-cytochrome c reductase activity in the liver decreased per mg DNA in protein deprivation but was unchanged per mg RNA of membrane-bound ribosomes.
D000818:Animals; D004247:DNA / Q000378:metabolism; D004044:Dietary Proteins / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism*; D009929:Organ Size; D011488:Protein Deficiency / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism; D051381:Rats; D012270:Ribosomes / Q000378:metabolism*
D004044:Dietary Proteins; D012335:RNA, Ribosomal; D004247:DNA; D009251:NADPH-Ferrihemoprotein Reductase