Regulation of the murine IgE antibody response. III. Analysis of the IgE response to insulin species variants.
IgE responses to insulin species variants i.e. to bovine, ovine and porcine insulins (respectively BI, OI and PI), were investigated. H-2d mice developed an anti-BI IgE response when this antigen was administered i.p. with A1(OH)3 gel, and H-2b and H-2a,d,k mice generated anti-BI and anti-OI IgE responses, respectively, when these antigens were injected i.p. with Freund's complete adjuvant (FCA). H-2d mice, on the other hand, developed only weak to negligible anti-PI IgE responses, when this antigen was administered in A1(OH)3 or in FCA. Antibody cross-reactivity indicated that at least two populations of IgE antibodies were produced in response to BI and/or OI; one population specific for the A chain loop and a second population specific for a determinant common to several insulins. Finally, i.v. injections of conjugates of insulin with spleen lymphocytes markedly suppressed the capacity of mice to develop an anti-insulin IgE response.
D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D002417:Cattle; D004306:Dose-Response Relationship, Immunologic; D005620:Freund's Adjuvant / Q000008:administration & dosage; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000145:classification; D007328:Insulin / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D012756:Sheep; D013045:Species Specificity; D013552:Swine
D000941:Antigens; D007328:Insulin; D007073:Immunoglobulin E; D005620:Freund's Adjuvant