Uptake of 5-HT by blood platelets of patients with myoclonus epilepsy.
5-HT uptake by blood platelets was tested in patients with PME with no Lafora bodies. Blood platelets from patients with myoclonus epilepsy and those from healthy sex and age matched controls were incubated for 5 min with 0.1 microM 3H-5-HT in Krebs-Henseleit buffer at 37 degrees C. The radioactivity taken up by platelets was assayed by liquid scintillation counting. 5-HT uptake in platelets from patients tended to be slightly higher (19%, n = 18, P greater than 0.05). The kinetic pattern as revealed by the Lineweaver-Burk plot, was very similar in the two groups. All patients were on antiepileptic drugs (sodium valproate, clonazepam and phenobarbital), but no evidence was found in vitro that these drugs would have any direct effect on the uptake.
D000293:Adolescent; D000328:Adult; D001693:Biological Transport, Active; D001792:Blood Platelets / Q000378:metabolism*; D004831:Epilepsies, Myoclonic / Q000097:blood*; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D012701:Serotonin / Q000097:blood*; D014316:Tritium
D014316:Tritium; D012701:Serotonin