An improved method for the retrieval of cultured macrophages.
We report a simple procedure for the maintenance and retrieval of rodent peritoneal macrophages on dialysis membrane supports. Culture units are easily constructed from plastic petri dishes and dialysis membranes. Macrophages adhere readily but are detached easily from the dialysis membrane by mild mechanical agitation. Cell viability remains high and the morphologic and functional characteristics of the macrophages are maintained.
D000818:Animals; D002448:Cell Adhesion; D002470:Cell Survival; D002478:Cells, Cultured; D007158:Immunologic Techniques; D008264:Macrophages / Q000032:analysis / Q000166:cytology / Q000276:immunology; D008567:Membranes, Artificial; D051381:Rats; D011919:Rats, Inbred Strains
D008567:Membranes, Artificial