Structural modification of human erythrocyte membranes following gamma-irradiation.
Structural damage to isolated erythrocyte membranes ('ghosts') has been studied following gamma-irradiation under a variety of conditions. For this two fluorescent probes were used; one 1-anilino-8-naphthalene sulphonate probes the lipid-aqueous interface, the other, diphenylhexatriene, was used to probe the membrane fluidity. Irradiation of the membranes caused a decrease in fluorescent intensity of the added probes, and changes in polarization of fluorescence. Oxygen was found to enhance the radiation damage, and scavenger experiments showed the hydroxyl radical was the major radical species involved. The structural modifications are therefore interpreted in terms of preliminary chemical damage involving peroxidation of unsaturated lipids. In addition sensitization and protection was observed in the presence of known dose-modifying chemicals.
D010129:4-Aminobenzoic Acid; D000817:Anilino Naphthalenesulfonates; D002245:Carbon Dioxide / Q000494:pharmacology; D003037:Cobalt Radioisotopes; D004161:Diphenylhexatriene; D004910:Erythrocyte Membrane / Q000032:analysis / Q000528:radiation effects*; D004912:Erythrocytes / Q000528:radiation effects*; D005454:Fluorescence Polarization; D005561:Formates; D005720:Gamma Rays; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007461:Iodoacetates; D019807:Iodoacetic Acid; D009584:Nitrogen; D009609:Nitrous Oxide; D010100:Oxygen / Q000494:pharmacology; D011829:Radiation Dosage; D012964:Sodium; D013050:Spectrometry, Fluorescence
D000817:Anilino Naphthalenesulfonates; D003037:Cobalt Radioisotopes; D005561:Formates; D007202:Indicators and Reagents; D007461:Iodoacetates; D002245:Carbon Dioxide; D004161:Diphenylhexatriene; D012964:Sodium; D009609:Nitrous Oxide; D009584:Nitrogen; D010100:Oxygen; D010129:4-Aminobenzoic Acid; D019807:Iodoacetic Acid