A rapid enzymic procedure for the determination of picomole amounts of UDP-glucuronic acid.
A simple microassay for the determination of UDP-glucuronic acid was developed on the basis of the formation of benzo[a]pyrene 3-glucuronide catalysed by UDP-glucuronyltransferase of guinea-pig liver. As little as 1-5 pmol of UDP-glucuronic acid was detectable in extracts of heat-denatured probes of liver or cultured cells equivalent to 10-50 micrograms of cellular protein.
D000818:Animals; D001580:Benzopyrenes; D002478:Cells, Cultured; D014453:Glucuronosyltransferase; D006168:Guinea Pigs; D008297:Male; D051379:Mice; D008832:Microchemistry / Q000379:methods; D008862:Microsomes, Liver / Q000032:analysis; D051381:Rats; D013050:Spectrometry, Fluorescence; D014535:Uridine Diphosphate Glucuronic Acid / Q000032:analysis*; D014539:Uridine Diphosphate Sugars / Q000032:analysis*
D001580:Benzopyrenes; D014539:Uridine Diphosphate Sugars; D014535:Uridine Diphosphate Glucuronic Acid; C021338:3-hydroxybenzo(a)pyrene; D014453:Glucuronosyltransferase