In vitro immune response of human peripheral lymphocytes. VII. Effect of anti-mu and anti-delta antibodies on B colony formation and detection of abnormal B cells in patients with juvenile rheumatoid arthritis.
Effect of anti-mu and anti-delta antibodies on PHA- and protein A-induced B colony formation was studied. Anti-mu antibody at any concentrations tested did not show inhibitory or enhancing effect on colony formation. On the other hand, anti-delta antibody enhanced both PHA- and protein A-induced colony formation. Optimum concentration of anti-delta antibody for maximum enhancement was 0.1 microgram/ml. and F(ab')2 fragment of anti-delta antibody also showed comparable enhancing effect. Simultaneous addition of IgD with anti-delta antibody abrogated anti-delta-induced enhancement, and anti-delta antibody did not show any facilitation of colony formation in IgM+ IgD- cell population. In marked contrast with normal B cells, anti-mu antibody showed a remarkable enhancing effect on protein A-induced colony formation of B cells from JRA patients. F(ab')2 fragment of anti-mu antibody also showed comparable enhancing effect. Anti-mu antibody did not show any enhancement of colony formation of B cells from several other autoimmune diseases. The result indicated the presence of abnormal B cells in JRA patients.
D000818:Animals; D000918:Antibody Specificity; D001171:Arthritis, Juvenile / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation; D003114:Colony-Forming Units Assay; D006801:Humans; D007111:Immunity, Cellular; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis*; D007138:Immunoglobulin delta-Chains / Q000096:biosynthesis*; D007148:Immunoglobulin mu-Chains / Q000096:biosynthesis*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000494:pharmacology
D007143:Immunoglobulin Heavy Chains; D007138:Immunoglobulin delta-Chains; D007148:Immunoglobulin mu-Chains; D010835:Phytohemagglutinins; D013205:Staphylococcal Protein A