Measurement and localization of angiotensin-like immunoreactivity in juxtaglomerular cells of the rat.
The existence and distribution of angiotensin I (A I) and angiotensin II (A II) in rat kidney were examined in immunocytochemical studies using the peroxidase-antiperoxidase technique and in biochemical studies using rat kidney homogenates extracted with acid-ethanol and purified by Sephadex G-25 gel chromatography. Immunopositive A II-like staining was observed in the juxtaglomerular cells of the afferent arteriole, but no histochemical evidence for A I was found. On the other hand, renal homogenates were found to contain both A I and A II immunoreactivities which coeluted on gel chromatography with synthetic A I and A II. These results indicate that A I as well as A II immunoreactivities are present in the kidney and that A II immunoreactivity can be localized to the juxtaglomerular cells. The origin of the immunoreactive A II in the juxtaglomerular cells remains to be determined.
D000803:Angiotensin I / Q000032:analysis*; D000804:Angiotensin II / Q000032:analysis*; D000809:Angiotensins / Q000032:analysis*; D000818:Animals; D002850:Chromatography, Gel; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000032:analysis / Q000166:cytology*; D007678:Kidney Glomerulus / Q000166:cytology; D008297:Male; D011863:Radioimmunoassay; D051381:Rats
D000809:Angiotensins; D000804:Angiotensin II; D000803:Angiotensin I