[Mitochondrial and lysosomal membrane stability following administration of excessive doses of vitamin A in vivo].
Stability of mitochondrial and lysosomal membranes was studied in growing rats under conditions of hypervitaminosis A. The membrane stability was evaluated by the rate of solubilization of marker mitochondrial enzyme--malate dehydrogenase, and of lysosomal enzyme--beta-galactosidase, using incubation of the isolated organelles with gradually increasing concentrations of Triton X-100. High doses of vitamin A did not affect the total activity of the enzymes studied but distinctly increased the rate of malate dehydrogenase and beta-galactosidase solubilization, caused by Triton X-100, as compared with controls. This suggests the increase in sensitivity of the organelle membranes to the effect of Triton X-100 in the hypervitaminosis A, which is apparently related to pronounced impairment of the membrane structure.
D000818:Animals; D006986:Hypervitaminosis A; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure*; D008247:Lysosomes / Q000201:enzymology*; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D001616:beta-Galactosidase / Q000378:metabolism
D011092:Polyethylene Glycols; D008291:Malate Dehydrogenase; D001616:beta-Galactosidase
Issledovanie stabil'nosti membran mitokhondriĭ i lizosom pri vvedenii izbytochnykh doz vitamina A in vivo.