Vitamin K-dependent gamma-carboxyglutamic acid formation by mouse renal adenocarcinoma cells (RAG).
Previous studies have identified gamma-carboxyglutamic acid as a constituent of one or more protein(s) synthesized by rat and chicken kidney microsomes in vitro in a vitamin K-dependent post-translational reaction [1]. Incubation of microsomes from a mouse kidney cell line (RAG) with [14C]NaHCO3 results in formation of protein-bound [14C]gamma-carboxylglutamic acid. Incorporation is stimulated threefold by addition of the active vitamin K compound 2-methyl, 3-farnesyl, 1,4-naphthoquinone. At least 90% of incorporated, nondialyzable [14C] is situated in the gamma-carboxyl group of gamma-carboxyglutamic acid residues.
D015055:1-Carboxyglutamic Acid / Q000096:biosynthesis*; D000230:Adenocarcinoma / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D019735:Carbon-Carbon Ligases; D005971:Glutamates / Q000096:biosynthesis*; D007668:Kidney; D007680:Kidney Neoplasms / Q000378:metabolism*; D008025:Ligases / Q000378:metabolism*; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism; D014176:Protein Biosynthesis; D014812:Vitamin K / Q000502:physiology*
D005971:Glutamates; D014812:Vitamin K; D015055:1-Carboxyglutamic Acid; D008025:Ligases; D019735:Carbon-Carbon Ligases; C020660:glutamyl carboxylase; D002118:Calcium