Loss of tritium from coprostanone derived from [1,2(n)-3H]cholesterol or [7(n)-3H]cholesterol.
After oral administration of a mixture of [1,2(n)-3H]cholesterol and [4-14C]cholesterol to a baboon, fecal coprostanone had a 46% lower 3H/14C ratio than the dose administered. Loss of 3H by enolization of the 3-ketone could account for the decrease in 3H/14C. If [7(n)-3H]cholesterol was administered instead of [1,2(n)-3H]cholesterol a 23% loss of 3H from coprostanone was found. Procedures requiring measurement of 3H-coprostanone derived from [1,2(n)-3H]- or [7(n)-3H]cholesterol could be seriously in error unless an appropriate correction for loss of 3H is made.
D000818:Animals; D002250:Carbon Radioisotopes / Q000032:analysis; D002776:Cholestanes / Q000032:analysis*; D004083:Cholestanol / Q000032:analysis; D002784:Cholesterol / Q000032:analysis / Q000378:metabolism*; D005051:Eubacterium / Q000378:metabolism; D005188:False Negative Reactions; D005243:Feces / Q000032:analysis; D000882:Haplorhini; D007664:Ketosteroids / Q000032:analysis; D008297:Male; D010215:Papio; D012855:Sitosterols / Q000378:metabolism; D014316:Tritium / Q000032:analysis / Q000378:metabolism*
D002250:Carbon Radioisotopes; D002776:Cholestanes; D007664:Ketosteroids; D012855:Sitosterols; D014316:Tritium; D004083:Cholestanol; D002784:Cholesterol; C007123:coprostanone