Effects of potassium sorbate and other antibotulinal agents on germination and outgrowth of Clostridium botulinum type E spores in microcultures.
The effects of potassium sorbate, sodium hypophosphite, sodium tripolyphosphate, sodium nitrite, and linoleic acid on the germination and outgrowth of Clostridium botulinum type E spores were studied in microcultures. At pH 5.8 to 6.0 in liver veal agar, the germination rate was decreased to nearly zero with 1.0, 1.5, or 2.0% sorbate. At pH 7.0 t 7.2, these levels of sorbate afforded germination and outgrowth of abnormally shaped cells that were defective in cell division. At the high pH range, 0.5 or 1.0% hypophosphite had effects similar to those of sorbate. The use of 0.05% sodium nitrite with sorbate enhanced the lysis of outgrowing cells at pH 7.2 or lower. Emergence and elongation were inhibited by 0.05% linoleic acid with or without 1.0% sorbate at pH 7.0 to 7.2. The addition of 0.5% tripolyphosphate to media containing 1.5% sorbate at pH 7.1 prevented normal cell growth to an extent greater than with sorbate alone.
D003014:Clostridium botulinum / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology*; D010721:Phosphinic Acids / Q000494:pharmacology*; D013011:Sorbic Acid / Q000494:pharmacology*; D013171:Spores, Bacterial / Q000187:drug effects
D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D010721:Phosphinic Acids; C009287:sodium hypophosphite; D019787:Linoleic Acid; D013011:Sorbic Acid