A clinical assessment of fluorescence polarisation changes in lymphocytes stimulated by phytohaemagglutinin (PHA) in malignant and benign diseases.
The modified double-zone SCM technique, developed in these laboratories as an in vitro test for cancer, is based on the differential response to PHA of lymphocytes harvested from two regions of a Ficoll-Triosil gradient. Lymphocyte responses are measured by changes in intracellular fluorescein fluorescence polarisation. We report its continued clinical evaluation in patients hospitalised with malignant and non-malignant diseases, including a blind trial of 78 patients with disorders of the gastrointestinal tract, breast and lung. Overall "false' negative and "false' positive rates from 336 blood samples were 1.8 and 3.3% respectively.
D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000175:diagnosis*; D002499:Centrifugation, Density Gradient; D002986:Clinical Trials as Topic; D003937:Diagnosis, Differential; D005260:Female; D005454:Fluorescence Polarization; D005767:Gastrointestinal Diseases / Q000175:diagnosis; D005770:Gastrointestinal Neoplasms / Q000175:diagnosis*; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis*; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000494:pharmacology; D012140:Respiratory Tract Diseases / Q000175:diagnosis
D010835:Phytohemagglutinins