Facilitated diffusion of 6-deoxy-D-glucose in bakers' yeast: evidence against phosphorylation-associated transport of glucose.
6-Deoxy-D-glucose, a structural homomorph of D-glucose which lacks a hydroxyl group at carbon 6 and thus cannot be phosphorylated, is transported by Saccharomyces cerevisiae via a facilitated diffusion system with affinity equivalent to that shown with D-glucose. This finding supports the facilitated diffusion mechanism for glucose transport and contradicts theories of transport-associated phosphorylation which hold that sugar phosphorylation is necessary for high-affinity operation of the glucose carrier.
D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D004058:Diffusion; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D010766:Phosphorylation; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D002352:Carrier Proteins; D003837:Deoxy Sugars; D003847:Deoxyglucose; D005947:Glucose