X-ray microprobe analysis of chloragosomes of untreated and of EDTA-treated Lumbricus terrestris by using fresh air-dried smears.
The chloragosomes of Lumbricus terrestris were analysed by an electron microscope X-ray microphobe, using both air-dried smear preparations and conventional electron microscopic sections. In untreated worms the air-dried chloragosomes showed prominent peaks for Ca and P, distinct peaks for S and Zn, while Na, K, and Cl peaks were barely detectable. In air-dried smears the S peak was constantly more prominent than in EM sections, indicating that chloragosomes contain easily soluble S-compounds. By long-term CaNa2EDTA treatment of the worms, the Ca and P content of the chloragosomes could be significantly decreased, empirically proving, that the storage and mobilisation of chloragosomal Ca and P are closely related.
D000818:Animals; D002118:Calcium / Q000032:analysis*; D004492:Edetic Acid / Q000494:pharmacology*; D004577:Electron Probe Microanalysis; D004602:Elements / Q000032:analysis; D009835:Oligochaeta / Q000032:analysis* / Q000648:ultrastructure; D009940:Organoids / Q000032:analysis; D010758:Phosphorus / Q000032:analysis*
D004602:Elements; D010758:Phosphorus; D004492:Edetic Acid; D002118:Calcium