Electron immunoperoxidase stainings in sections of unfixed pure cultures of Streptococcus mutans.
Unfixed as well as glutaraldehyde-fixed pelleted cells from pure cultures of Streptococcus mutans subspecies sobrinus (B13) were embedded in glycolmethacrylate. Ultrathin were sections immunoperoxidase labelled with rabbit anti-S. mutans sobrinus gamma-globulin. It was found that unfixed specimens became labelled as precisely and as distinctly as did the fixed specimens. All controls were negative.
D005404:Fixatives / Q000008:administration & dosage; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D013295:Streptococcus mutans / Q000166:cytology* / Q000648:ultrastructure
D005404:Fixatives