The use of human lymphoid cell line lymphokine preparations (LCL-LK) as working standards in the bioassay of human leucocyte migration inhibition factor (LIF).
The leucocyte migration inhibition test in agarose as described by Clausen (1971) was modified into a statistically designed assay of LIF activity using human polymorphonuclear leucocytes from single blood donors. Individual assays included a laboratory standard of lymphokine with LIF activity prepared from the culture supernatants of the RPMI 1788 human lymphoblastoid cell line (LCL-LK). Analysis of 157 LIF assays revealed simple criteria by which the acceptability of an individual assay could be judged before subjecting it to statistical analysis. The failure of LIF assays to meet these criteria of acceptability was particularly associated with low areas of control polymorph migration in the absence of lymphokine ('spontaneous migration'). We demonstrate that the statistically designed assay permits the measurement, with precision, of LIF activity in units/ml by reference to a working standard of LCL-LK. We illustrate the use of this assay in the measurement of LIF activity generated by tuberculin-stimulated human peripheral blood lymphocytes.
D000818:Animals; D002460:Cell Line; D002464:Cell Migration Inhibition; D004306:Dose-Response Relationship, Immunologic; D006168:Guinea Pigs; D006801:Humans; D007961:Leukocyte Migration-Inhibitory Factors / Q000032:analysis* / Q000494:pharmacology; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000032:analysis* / Q000096:biosynthesis / Q000592:standards*; D009504:Neutrophils / Q000276:immunology; D012015:Reference Standards; D014373:Tuberculin / Q000276:immunology
D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D014373:Tuberculin