An enzyme-linked immunosorbent assay (ELISA) for measurement of heterophile antibody.
An enzyme-linked immunosorbent assay (ELISA) test has been developed for measurement of heterophile antibody. The microtiter test utilizes a bovine erythrocyte monolayer as antigen and anti-human IgM antiserum conjugated with horseradish peroxidase to measure the degree of binding of the heterophile antibody in the test serum with the erythrocytes. A single serum dilution yields quantitative results when read in a spectrophotometer. The ELISA test showed a sensitivity comparable with the immune adherence hemagglutination assay (IAHA) and other heterophile tests, good reproducibility, and high specificity.
D000328:Adult; D000910:Antibodies, Heterophile / Q000032:analysis*; D003080:Cold Temperature; D004355:Drug Stability; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests; D006801:Humans; D007124:Immunoenzyme Techniques; D007244:Infectious Mononucleosis / Q000276:immunology*
D000910:Antibodies, Heterophile