Fibrinolytic activity of salivary euglobulin fractions precipitated at pH 5.9 or 6.4 before and after mixing with blood.
Mixed saliva was collected from 20 healthy young persons before oral surgery, immediately after the operation and at control 5 days later. The fibrinolytic activity of untreated whole saliva and salivary euglobulin fractions precipitated at pH 5.9 (10 patients) or at pH 6.4 (10 patients) was measured on human fibrin plates. The salivary euglobulin fractions were also processed on plasminogen-free fibrin plates. The concentrations of alpha 1-antitrypsin, antithrombin III, C1-esterase inhibitor, alpha 2-macroglobulin, alpha 2-plasmin inhibitor and plasminogen were measured in pooled plasma and four postoperative saliva samples before and after preparing the corresponding dissolved euglobulins precipitated at pH 5.9 or pH 6.4. Dissolved euglobulins from the pooled plasma were subjected to crossed immunoelectrophoresis with antisera to plasminogen in the second-dimension gel. Normal mixed saliva showed unaltered fibrinolytic activity after euglobulin precipitation, whereas euglobulins prepared from postoperative samples had higher activity than the untreated specimens. No significant differences of fibrinolytic activity between salivary euglobulins precipitated at pH 5.9 and 6.4 were demonstrated. The recovery of proteinase inhibitors in the euglobulin fractions of plasma is lower than 2.1% except C1-esterase inhibitor, which is precipitated, but only at pH 5.9. The mixing with saliva does not seem to change this relative quantity of precipitated inhibitors from plasma. Plasminogen precipitated from plasma is not proteolytic degraded and the yield is higher at pH 5.9 than at pH 6.4. Salivary euglobulins precipitated at pH 5.9 or 6.4 are superior to untreated saliva to measure the fibrinolytic activity of mixed saliva, especially in samples contaminated with blood.
D001769:Blood; D011232:Chemical Precipitation; D005342:Fibrinolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007158:Immunologic Techniques; D012471:Salivary Proteins and Peptides / Q000032:analysis / Q000502:physiology*; D012712:Serum Globulins / Q000032:analysis / Q000502:physiology*
D012471:Salivary Proteins and Peptides; D012712:Serum Globulins