Multiparameter appraoch to the "fingerprinting" of fluorescent Pseudomonads.
A recent study has shown that an emission-excitation matrix could be used to provide a unique fingerprint for the selective identification and characterization of certain species of Pseudomonas. This paper describes the results of a systematic study of the variables that contribute to the uniqueness of the fingerprint. Additional insight concerning the nature of the fluorescent pigments of these species is gained by analysis with a high-performance liquid chromatograph/video fluorometer combination. While providing information about the analytical variables contributing to the analysis, this combination also added the variable of chromatographic retention time for unambiguous fingerprinting.
D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid; D003201:Computers; D005452:Fluoresceins; D049711:History, Modern 1601-; D010860:Pigments, Biological / Q000032:analysis*; D011549:Pseudomonas / Q000032:analysis*; D011550:Pseudomonas aeruginosa / Q000032:analysis; D011551:Pseudomonas fluorescens / Q000032:analysis; D013045:Species Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
D005452:Fluoresceins; D010860:Pigments, Biological