Successful allotransplantation of mouse pancreatic islets to nonimmunosuppressed recipients.
Pancreatic islets from BALB/c (H-2d) mice are rejected within 14 days of transplantation to the kidney capsule of allogeneic, CBA/H (H-2k) recipients. Cyclophosphamide pretreatment of the islet donor reduced the intensity of the allograft response, and these islets undergo a more chronic rejection process. Islets from cyclophosphamide-pretreated donors can be cultured in a gas phase of 95% O2 and 5% CO2, provided the islets are aggregated into clusters of about 50 islets. After a culture period of 7--12 days, the islet tissue was transplanted to normal allogeneic recipients, where the tissue elicited little or no allograft response over a 3-mo observation period.
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology; D006085:Graft Survival / Q000187:drug effects; D006789:Host vs Graft Reaction; D006801:Humans; D007165:Immunosuppression Therapy; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects; D016381:Islets of Langerhans Transplantation; D051379:Mice; D008815:Mice, Inbred Strains; D009924:Organ Culture Techniques; D014184:Transplantation, Homologous
D003520:Cyclophosphamide