Serological and genetic identification of a bovine B lymphocyte alloantigen system.
A two-colour fluorescence microcytotoxicity test was used to screen antisera for antibodies specific for bovine B lymphocytes. A total of 114 cattle alloantisera were screened against peripheral blood lymphocytes from 100 unrelated individuals. Anti-B lymphocyte activity was detected in 47 antisera. Cytotoxic antibodies to antigens other than B lymphocyte specific antigens were removed by absorbing the antisera with buffy coat cells or platelets isolated from whole blood. Selected antisera were used to type paternal half-sib families. The presence of a polymorphic, MHS-linked antigen system on B lymphocytes was demonstrated. The tissue distribution and MHS linkage of these antigens suggests this system is analogous to the class II or Ia antigens of other species.
D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology; D002417:Cattle; D003602:Cytotoxicity, Immunologic; D004912:Erythrocytes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D007519:Isoantigens / Q000235:genetics*; D007962:Leukocytes / Q000276:immunology; D008285:Major Histocompatibility Complex
D007519:Isoantigens