[Evaluation of three commercial kits for the estimation of total glycolysed hemoglobin: Hb Al (author's transl)].
It has been demonstrated by numerous workers that variations in levels of glycolysed hemoglobins Hb Al (Alb, Alb, Alc) are an excellent means of control of the quality of diabetes control. The estimation of the main glycolysed hemoglobin (Hb Alc) is very difficult, and various chromatographic methods have been developed to permit rapid estimation of all these glycolysed hemoglobins. Our work consisted of testing the three microcolumn kits on the french market: Isolab, Helena and Biorad. The great thermo-dependency of the first two kits did not permit us to obtain results in correlation with those of our reference technic derived from Trivelli's method. On the other hand, the levels of Hb Al obtained with the Biorad kit were perfectly correlated with the levels of Hb Alc. The average normal of Hb Al was 6,45 +/- 0,66 p. cent, CV 10,2 p. cent; the average value of Hblc was 5,4 +/- 0,4 p. cent. CV 7,4 p. cent. The average levels of Hb A obtained in diabetic subjects was 12 p. cent, the levels of Hblc being 10 p. cent. Certain restrictions in the use of these microcolumns were demonstrated: presence of Hb F or abnormal Hb, hyperlipemia and presence of chylomicrons.
D000293:Adolescent; D000328:Adult; D001800:Blood Specimen Collection; D002845:Chromatography; D003920:Diabetes Mellitus / Q000097:blood*; D004492:Edetic Acid; D005260:Female; D006027:Glycosides / Q000032:analysis*; D006441:Hemoglobin A / Q000031:analogs & derivatives* / Q000032:analysis*; D006493:Heparin; D006801:Humans; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D013696:Temperature
D006027:Glycosides; D011933:Reagent Kits, Diagnostic; D006493:Heparin; D006441:Hemoglobin A; D004492:Edetic Acid
Evaluation de trois trousses commerciales pour le dosage de l'hémoglobine glycosylée totale : Hb Al.