Immunogenicity and molecular characterisation of staphylococcal delta haemolysin.
In rabbit antisera to staphylococcal delta haemolysin the haemolysin-neutralising activity was associated with the IgG fraction as well as with the alpha and beta lipoproteins. On an equal-weight basis, the neutralising capacity of specific antibody was not significantly greater than that of serum alpha or beta lipoproteins. Anti-alpha haemolysin was not detected in any of the anti-delta-haemolysin antisera. Amino-acid analysis of delta haemolysin showed that lysine, aspartic acid, threonine and isoleucine were the predominant amino acids present; histidine, arginine, proline, cysteine and tyrosine were absent. The molecular weight of delta-haemolysin in phosphate-buffered saline was approximately 210 000.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D006460:Hemolysin Proteins / Q000032:analysis / Q000276:immunology*; D007106:Immune Sera / Q000032:analysis; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000276:immunology*; D008074:Lipoproteins / Q000276:immunology*; D008970:Molecular Weight; D009500:Neutralization Tests; D011817:Rabbits; D013211:Staphylococcus aureus / Q000276:immunology*
D000596:Amino Acids; D000907:Antibodies, Bacterial; D006460:Hemolysin Proteins; D007106:Immune Sera; D007074:Immunoglobulin G; D008074:Lipoproteins