Clastogenic effect of 4-nitroquinoline-1-oxide on swine urothelial cells in culture.
Swine bladder epithelial cells in culture were treated with 4-nitroquinoline-1-oxide (4NQO) for 4 hr with three different concentrations to examine dose-dependent effects. To study time course effects, they were treated with one concentration for 4 hr and then incubated in 4NQO-free medium for three different periods before harvesting. Three different effects were recorded: (i) a suppression of mitotic activity that was dose-dependent and which continued for more than 30 hr posttreatment; (ii) no marked changes in the proportions of different ploidy classes; and (iii) chromatid gaps, breaks, and exchanges that were dose-dependent. This clastogenic effect decreased with increasing time after treatment.
D015112:4-Nitroquinoline-1-oxide; D000818:Animals; D002273:Carcinogens; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008938:Mitosis / Q000187:drug effects; D009600:Nitroquinolines; D011003:Ploidies / Q000187:drug effects; D001743:Urinary Bladder / Q000166:cytology* / Q000187:drug effects
D002273:Carcinogens; D009600:Nitroquinolines; D015112:4-Nitroquinoline-1-oxide