The thyroid-stimulating antibody of Graves' disease: evidence for restricted heterogeneity.
The thyroid-stimulating antibody (TSAb) of Graves' disease was shown to have a relatively constant pH on preparative isoelectric focusing; the fraction of pH 8.5-9.0 was maximal in thyroid-stimulating potency but inhibition of thyrotropin-binding (another property of Graves' disease IgG) was most marked with pH 9.5-10.6 fraction. H and L chains of TSAb-IgG were separated and H original (HO) was combined with L original (LO) and with Bence Jones L kappa and L lambda. Only HO + LO restored (minimal) bioactivity. Of isolated TSAb-IgG kappa and TSAb-IgG lambda only IgG lambda was thyroid stimulating. Sub-classes (IgG3 and IgG 1 + 2 + 4) of TSAb-IgG were prepared and with all of 6 sera TSAb was in IgG 1 + 2 + 4 with none in IgG3. These data combine to suggest heterogeneity of this autoimmune antibody.
D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis*; D001536:Bence Jones Protein / Q000032:analysis; D001681:Biological Assay; D006111:Graves Disease / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007143:Immunoglobulin Heavy Chains / Q000032:analysis; D007147:Immunoglobulin Light Chains / Q000032:analysis; D007525:Isoelectric Focusing; D008135:Long-Acting Thyroid Stimulator / Q000276:immunology; D013961:Thyroid Gland / Q000276:immunology*; D013972:Thyrotropin / Q000037:antagonists & inhibitors
D001323:Autoantibodies; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D013972:Thyrotropin; D001536:Bence Jones Protein; D008135:Long-Acting Thyroid Stimulator