Mass spectrometric determination of cytokinins in the top of tobacco plants using deuterium-labeled standards.
Penta-deuterated zeatin, ribosylzeatin, and glucosylzeatin were prepared by the condensation of 6-chloropurine, 9-beta-D-ribosyl- and 9-beta-D-glucosyl-6-chloropurine, and 4-hydroxy-3-methyl-2-butenylamine-d5 derived from acetone-d6. The deuterium contents of these compounds were approximately 96%. Zeatin, ribosylzeatin, N6-isopentenyladenosine and 2-methylthio-ribosylzeatin in the top of tobacco plants were quantitated by mass spectrometry using deuterium-labeled standards.
D003583:Cytokinins / Q000032:analysis*; D003903:Deuterium; D013058:Mass Spectrometry / Q000379:methods; D010937:Plant Growth Regulators / Q000032:analysis*; D010947:Plants, Toxic; D011865:Radioisotope Dilution Technique; D013329:Structure-Activity Relationship; D014026:Nicotiana / Q000032:analysis*
D003583:Cytokinins; D010937:Plant Growth Regulators; D003903:Deuterium