Accessory function of human tumor cell lines. I. Production of interleukin 1 by the human histiocytic lymphoma cell line U-937.
The established human histiocytic lymphoma cell line U-937 spontaneously produced a factor with biological activity similar to that ascribed to interleukin 1 (IL 1). Actually, supernatants from U-937 cells promoted proliferation of thymocytes initiated by concanavalin A (Con A) and replaced the requirement of accessory cells for activation of highly purified circulating T lymphocytes induced by Con A. Phorbol myristate acetate (PMA) significantly increased the titers of the helper factor produced by U-937 cells as compared to that secreted by non-PMA-treated U-937 cells or PMA-stimulated P388D1 murine macrophage tumor cells. Generally U-937 cells did not secrete detectable IL 1 activity during the first 24-48 h of culture. However, after this initial period the level of IL 1 activity increased and reached a maximum at 5-6 days of culture. Finally, the helper factor released by U-937 cells had an apparent mol. wt. of 12000-15000 as determined by Sephadex G-100 chromatography and lacked interleukin 2 activity as shown by its inability to support growth of IL 2-addicted T cell lines. To our knowledge this is the first report of an established human cell line capable of producing IL 1.
D000818:Animals; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis* / Q000494:pharmacology; D008213:Lymphocyte Activation; D016403:Lymphoma, Large B-Cell, Diffuse / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007375:Interleukin-1; D003208:Concanavalin A; D013755:Tetradecanoylphorbol Acetate