[Stability of the biocatalysts of L-aspartic acid synthesis based on immobilized Escherichia coli cells].
Experiments were carried out to investigate the process of a continuous enzymatic synthesis of L-aspartic acid from ammonium fumarate in uniform filling flow reactors. Escherichia coli (Soviet strain 85) cells immobilized in polyacrylamide gel granules reinforced by a solid carrier were used as biocatalysts. The conditions, under which a high aspartase activity of the biocatalyst and a stable hydrodynamic performance of the reactor were maintained, were determined. The main kinetic characteristics of a continuous performance of the reactor for 150 days were obtained.
D000180:Acrylic Resins; D001220:Aspartate Ammonia-Lyase / Q000378:metabolism; D001224:Aspartic Acid / Q000138:chemical synthesis*; D002384:Catalysis; D004355:Drug Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D005650:Fumarates / Q000378:metabolism; D007700:Kinetics; D008433:Mathematics; D013997:Time Factors
D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D005650:Fumarates; C016680:polyacrylamide gels; D001224:Aspartic Acid; C032005:fumaric acid; D001220:Aspartate Ammonia-Lyase
Stabil'nost' biokatalizatorov sinteza L-asparaginovoĭ kisloty na osnove immobilizovannykh kletok Escherichia coli.