Effect of the cutting procedure on the vitality of corneal endothelium in donor material.
In an experimental study using freshly enucleated pig's eyes, we investigated the endothelial cell loss on the cut edge. Remote-control rotating trephine was found to be the least injuring when it was applied to fresh and preserved human corneas. Corneal culture does not seem to enhance endothelial cell damage due to trephination.
D000818:Animals; D002452:Cell Count / Q000295:instrumentation / Q000379:methods; D003315:Cornea / Q000166:cytology; D016039:Corneal Transplantation; D004727:Endothelium / Q000166:cytology; D009926:Organ Preservation; D013552:Swine