Physicochemical characteristics of Listeria specific antigen 2.
Listeria specific antigen 2 (Ag2) was purified to within 97% of homogeneity, with a high yield, using both gel filtration and polyacrylamide gel electrophoresis. Ag2 is a glycoprotein. Its isoelectric point is about 4.2. As determined by sodium dodecyl sulphate-polyacrylamid gel electrophoresis, its molecular weight in 16710 +/- 450. Ag2 may aggregate easily since it was previously found in gel filtration in a peak corresponding to a molecular weight of 160000. No enzyme activity has been found in Ag2.
D000596:Amino Acids / Q000032:analysis; D000942:Antigens, Bacterial / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D008089:Listeria monocytogenes / Q000201:enzymology / Q000276:immunology*; D008970:Molecular Weight
D000596:Amino Acids; D000942:Antigens, Bacterial