The effect of anti-Ia antibody on immunoglobulin-secreting cells from healthy individuals and myeloma patients.
Immunoglobulin-secreting cells (ISC) in the peripheral blood of healthy individuals and in the bone marrow, peripheral blood, or plasmacytomas of patients with multiple myeloma were enumerated by a protein-A plaque-forming cell assay after treatment with anti-Ia antibody and complement. Rabbit anti-human B-cell antisera and monoclonal anti-Ia antibody (OKIal) were used. By this treatment, the number of ISC in the peripheral blood of healthy individuals decreased to half, and that of M-protein-secreting cells from some patients with multiple myeloma also decreased markedly. In one patient, the number of M-protein-secreting cells in the bone marrow were markedly reduced by this treatment, but this was not the case after 6 months of chemotherapy, suggesting that the chemotherapy reduced chiefly the Ia-positive myeloma cells rather than Ia-negative myeloma cells.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D001798:Blood Proteins / Q000096:biosynthesis; D003165:Complement System Proteins; D006462:Hemolytic Plaque Technique; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins; D007518:Isoantibodies / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000276:immunology*
D001798:Blood Proteins; D000949:Histocompatibility Antigens Class II; D007136:Immunoglobulins; D007518:Isoantibodies; C009823:M-proteins (Myeloma); D003165:Complement System Proteins