Human alpha-1-antichymotrypsin enhances primary antibody response in the mouse.
The effect of human alpha-1-antichymotrypsin (alpha-1-Achy) on antibody response was studied in mice. alpha-1-Achy increased the number of antisheep erythrocyte antibody-producing cells. The increase was dependent on the dose of alpha-1-Achy injected (from 0.25 to 1 mg par mouse). alpha-1-Achy was effective if injected 2 days before or simultaneously with sheep erythrocytes. Asialylated alpha-1-Achy also enhanced the antibody response in the same way as native alpha-1-Achy. When alpha-1-Achy was heated at 60 degrees C for 15 min, it appeared to maintain immunoenhancing activity. However, when treated at 70 degrees C for 15 min, an intermediate immunoenhancing activity was observed, and heating at 100 degrees C for 15 min resulted in loss of activity.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000921:Antibody-Producing Cells / Q000187:drug effects / Q000276:immunology; D002918:Chymotrypsin / Q000008:administration & dosage / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D006462:Hemolytic Plaque Technique; D006358:Hot Temperature; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012709:Serum Albumin / Q000008:administration & dosage; D012794:Sialic Acids / Q000378:metabolism; D000514:alpha 1-Antichymotrypsin
D012709:Serum Albumin; D012794:Sialic Acids; D000514:alpha 1-Antichymotrypsin; D002918:Chymotrypsin