DNA isolated from Mycobacterium leprae: genome size, base ratio, and homology with other related bacteria as determined by optical DNA-DNA reassociation.
DNA derived from Mycobacterium leprae (grown in armadillos) was isolated, purified, and analyzed spectrophotometrically. The genome size and guanine-plus-cytosine content of M. leprae were 1.3 X 10(9) and 55.8%, respectively. Among selected strains of mycobacterial, nocardial and corynebacterial species, Corynebacterium sp. 2628 LB, isolated from a human leprosy patient, showed the highest DNA homology with M. leprae. Of the DNAs derived from mycobacteria, those of M. tuberculosis and M. scrofulaceum showed a comparatively high reassociation with the DNA of M. leprae.
D000818:Animals; D001135:Armadillos / Q000382:microbiology; D003352:Corynebacterium / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D005798:Genes, Bacterial; D006801:Humans; D007918:Leprosy / Q000382:microbiology; D009161:Mycobacterium / Q000235:genetics; D009166:Mycobacterium leprae / Q000235:genetics* / Q000302:isolation & purification; D009615:Nocardia / Q000235:genetics; D013053:Spectrophotometry / Q000379:methods
D004269:DNA, Bacterial