The topography of the 5' end of 16-S RNA in the presence and absence of ribosomal proteins S4 and S20.
A ribonucleoprotein prepared by strong ribonuclease digestion of a complex of 16-S ribosomal RNA and proteins S4 and S20 from Escherichia coli has been characterized; its nucleotide sequence, the positions of enzyme cuts and the sequence excisions have been placed in the completed sequence of 16-S RNA. The positions and yields of enzyme cuts, and excisions of sequence, are compared with those of various ribonucleoproteins prepared with S4 or S20 alone, and with the ribonuclease-resistant S4 RNA prepared from renatured 16-s RNA in the absence of ribosomal protein. These data yield important information on the topography and organisation of the 5' third of the 16-s RNA which is selectively maintained in its native conformation by the bound proteins; they also provide criteria for testing secondary structural models of this region of 16-S RNA.
D001483:Base Sequence; D004926:Escherichia coli / Q000032:analysis; D009690:Nucleic Acid Conformation; D009698:Nucleoproteins; D012335:RNA, Ribosomal; D006163:Ribonuclease T1; D012261:Ribonucleoproteins; D012269:Ribosomal Proteins; D012270:Ribosomes / Q000032:analysis
D009698:Nucleoproteins; D012335:RNA, Ribosomal; D012261:Ribonucleoproteins; D012269:Ribosomal Proteins; D006163:Ribonuclease T1