Histidine decarboxylase. Purification from fetal rat liver, immunologic properties, and histochemical localization in brain and stomach.
Histidine decarboxylase from fetal rat liver was purified to near-homogeneity. The purified enzyme has a molecular weight of 210,000, and appears to contain two subunits with molecular weights of 145,000 and 66,000, respectively. The enzyme is inhibited by heavy metals such as Hg2+ and Zn2+ and sulfhydryl-reactive compounds such as 5,5'-dithiobis-2-nitrobenzoic acid. The enzyme is partially dependent on exogenous pyridoxal phosphate. Extensive dialysis results in 50% loss of enzyme activity which can be fully recovered by adding pyridoxal phosphate. Affinity of pyridoxal phosphate for the apoenzyme is 0.1 microM at pH 6.8. Antibody against purified histidine decarboxylase was raised in rabbits. The antibody has been employed in immunohistochemical studies to visualize histidine decarboxylase containing cells and neuronal processes in rat stomach and brain, respectively. Immunologic studies indicate that histidine decarboxylase from brain, gastric mucosa, and fetal rat liver share common antigenic properties.
D000818:Animals; D000936:Antigen-Antibody Complex; D001921:Brain / Q000201:enzymology*; D002262:Carboxy-Lyases / Q000302:isolation & purification*; D005260:Female; D005333:Fetus; D005455:Fluorescent Antibody Technique; D006640:Histidine Decarboxylase / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D005779:Immunodiffusion; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D011247:Pregnancy; D011863:Radioimmunoassay; D051381:Rats; D013270:Stomach / Q000201:enzymology
D000936:Antigen-Antibody Complex; D002262:Carboxy-Lyases; D006640:Histidine Decarboxylase