Confirmation of EMIT benzodiazepine assay with GLC/NPD.
A simple, rapid, and reliable gas-liquid chromatographic (GLC) confirmation procedure was developed for urine samples found positive by the EMIT-dau benzodiazepine metabolite assay. The procedure involves acid hydrolysis, organic extraction, and identification using GLC-nitrogen-phosphorus detection (NPD). The method was validated in three subjects who took 10 mg diazepam daily for five days. Urinary excretion of the diazepam related substances was monitored quantitatively for 12 days. Considerable differences in diazepam metabolism was observed, despite the small number of subjects used. The data further indicated that both the physical characteristics and the metabolic profile of each individual may determine whether or not their occasional benzodiazepine use would be detected by the EMIT procedure. In some individuals taking daily diazepam, 48 to 72 hours may be required before sufficient metabolites accumulate in the urine to give a positive EMIT reaction.
D000328:Adult; D001569:Benzodiazepines / Q000652:urine*; D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male
D001569:Benzodiazepines