Possibility of detection of an in vitro expression of a group of human cancer-prone genes by automated methodologies.
Increased in vitro hyperdiploidy has been considered to be an in vitro expression of some cancer-prone genes in human dermal monolayer cultures. Examination of metaphases in chromosome preparations does not lend itself to studies on either large high-risk kindreds or populations. It is postulated that increased in vitro hyperdiploidy should be detected by 2 different automated assays for cellular DNA content: (a) incorporation of [3H]thymidine during logarithmic growth and (b) the occurrence (%) of cells with double (4n) and greater than double (greater than 4n) DNA content by flow cytophotometry. Concurrence between these two assays and with the metaphase assay should make feasible the use of this in vitro abnormality in the identification of individuals at increased cancer risk.
D000293:Adolescent; D000328:Adult; D003201:Computers; D004171:Diploidy; D005260:Female; D005821:Genetic Techniques; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008677:Metaphase; D008875:Middle Aged; D009369:Neoplasms / Q000235:genetics*; D009857:Oncogenes; D012306:Risk; D012867:Skin / Q000166:cytology