Genes for three Drosophila heat-shock-induced proteins at a single locus.
Two plasmids containing cDNA inserts complementary to the messenger RNAs for the 26,000 and 23,000 molecular weight heat-shock-induced proteins from Drosophila melanogaster were identified. Both of these probes hybridized to a site of a major heat-shock-induced puff, 67B. Hybridization by the gel transfer technique with genome DNA that had been cleaved by a restriction endonuclease indicated that the two genes are closely linked. This expectation was validated through isolation of a DNA fragment generated by BamHI endonuclease containing genes for the 26,000 and 23,000 molecular weight heat-shock-induced proteins and also a gene encoding the 27,000 molecular weight heat-shock-induced protein.
D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure; D004274:DNA, Recombinant; D004331:Drosophila melanogaster / Q000235:genetics*; D005796:Genes; D008040:Genetic Linkage; D006358:Hot Temperature; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011506:Proteins / Q000235:genetics*
D004274:DNA, Recombinant; D011506:Proteins