Immunobead enzyme-linked immunosorbent assay for quantitating immunoglobulin A in human secretions and serum.
We have developed an enzyme-linked immunosorbent assay for quantitating human immunoglobulin A in human secretions and serum. The procedure uses Immunobeads as the solid phase, has a sensitivity of 10 ng/ml, and can be completed in 1 day. Since there is no diffusion through agar, the assay does not require a correction factor when measuring secretory immunoglobulin A. This method should be useful in studies of local mucosal immunity in humans, especially when a very sensitive assay is needed.
D002771:Cholera / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000276:immunology; D005260:Female; D006801:Humans; D005779:Immunodiffusion; D007070:Immunoglobulin A / Q000032:analysis*; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D007413:Intestinal Mucosa / Q000276:immunology*; D008895:Milk, Human / Q000276:immunology*; D012463:Saliva / Q000276:immunology*; D007507:Therapeutic Irrigation
D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory