Insulin release from mouse islets perifused with serum IgG from newly insulin-dependent diabetics.
In the present study we have investigated the effect of IgG isolated from sera of newly diagnosed insulin-dependent diabetic patients (IDD-IgG) on insulin release of column-perifused isolated mice islets of Langerhans in the presence and absence of guinea pig complement. The insulin release is highly significantly (p less than 0.01) increased by IDD-IgG as compared with normal serum IgG in the presence of untreated as well as heat-inactivated GPNS. However, complement exposure suppressed insulin release significantly (p less than 0.01) in each period of perifusion. After perifusion in the presence of complement the arginine-stimulated insulin secretion was 6 times lower than without complement exposure of perifused islets before.
D000818:Animals; D003165:Complement System Proteins / Q000276:immunology; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D006168:Guinea Pigs; D006801:Humans; D007074:Immunoglobulin G / Q000008:administration & dosage* / Q000302:isolation & purification; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C
D007074:Immunoglobulin G; D007328:Insulin; D003165:Complement System Proteins