Concentration of baboon endogenous virus in large-scale production by use of hollow-fiber ultrafiltration technology.
A process is described in which the baboon endogenous virus (BaEV) is produced under optimum conditions in cell culture, and concentrated by hollow-fiber ultrafiltration technology under conditions of large-scale production. This system has advantages over conventional systems in that the flow rate is increased 2.5-fold during concentration. Thermal inactivation of BaEV was retarded by the addition of lactose glutamate to the harvested tissue culture fluid. After concentration, at least 91% of the virus RNA-directed DNA polymerase is recovered with a concomitant increase in infectious virus. Materials needed for modifying described systems may be obtained from commercial sources.
D000818:Animals; D002460:Cell Line; D003080:Cold Temperature; D004285:Dogs; D000882:Haplorhini; D013677:Medical Laboratory Science; D010215:Papio; D013950:Thymus Gland / Q000196:embryology; D014462:Ultrafiltration; D014775:Virus Activation; D014776:Virus Cultivation / Q000379:methods*; D014780:Viruses