Isoniazid hepatoxicity: the relationship between covalent binding and metabolism in vivo.
The relationship between the hepatotoxicity and metabolism of isoniazid and its metabolites, acetylisoniazid and acetylhydrazine, has been investigated. Toxic doses of acetylisoniazid and acetylhydrazine, radiolabeled in the acetyl group, were found to bind covalently to liver protein in vivo. This binding was mediated by the microsomal enzyme system as indicated by the effects of pretreatments altering the activity of these enzymes. Metabolic studies revealed that the pretreatments increased the metabolism of the acetylhydrazine moiety of acetyl-labeled acetylisoniazid and of acetylhydrazine itself by the microsomal enzyme system. Pretreatment with the acyl amidase inhibitor, bis-p-nitrophenyl phosphate, inhibited the hydrolysis of acetylisoniazid to isonicotinic acid plus acetylhydrazine and concomitantly decreased the covalent binding of acetyl-labeled acetylisoniazid. The changes in the metabolism of isoniazid, acetylisoniazid and acetylhydrazine effected by various pretreatments paralleled changes in the severity of the hepatic necrosis caused by the compounds. These results strongly suggest that acetylhydrazine is the metabolite responsible for the hepatic necrosis caused by isoniazid and that mirosomal metabolism of acetylhydrazine in vivo leads to the production of a reactive acylating species capable of reacting covalently with tissue macromolecules.
D000107:Acetylation; D000581:Amidohydrolases / Q000037:antagonists & inhibitors; D000818:Animals; D001711:Biotransformation / Q000187:drug effects; D002245:Carbon Dioxide / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology*; D003035:Cobalt / Q000494:pharmacology; D006868:Hydrolysis; D007538:Isoniazid / Q000009:adverse effects / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D009596:Nitrophenols / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010634:Phenobarbital / Q000494:pharmacology; D011485:Protein Binding; D051381:Rats
D009596:Nitrophenols; D009943:Organophosphorus Compounds; D002245:Carbon Dioxide; D003035:Cobalt; C002887:bis(4-nitrophenyl)phosphate; D000581:Amidohydrolases; C019665:amidase; D007538:Isoniazid; D010634:Phenobarbital