Blood transfusions as "transplantation pretreatment": incidence of immunization against unfractionated lymphocytes, T- and B-lymphocytes.
Since May 1980 patients waiting for cadaveric kidney grafts have been pretreated with at least 5 units of leukocyte poor blood. In this study we have analysed the occurrence of antibodies against unfractionated cells, T and B cells following these transfusions. Of the previously untransfused nineteen patients none formed cytotoxic T cell antibodies while warm B cell antibodies were found in sera from three patients, one being broadly cytotoxic killing also unfractionated lymphocytes. Broadreactive cold B cell antibodies were found in serum from one patient. Of the previously transfused fourteen patients none formed T cell antibodies following transfusions with the "lacking" blood units, while two patients formed warm B cell antibodies. Thus, immunization against B cells was more common than immunization against T cells. The significance of these B cell antibodies is unclear, but these antibodies did not show anti-HLA-DR specificity.
D000328:Adult; D000368:Aged; D000740:Anemia / Q000628:therapy; D000961:Antilymphocyte Serum / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007114:Immunization; D016030:Kidney Transplantation; D008875:Middle Aged; D011300:Preoperative Care; D013601:T-Lymphocytes / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D065227:Transfusion Reaction
D000961:Antilymphocyte Serum; D006680:HLA Antigens