[Obtaining an anti-trout (Salmo gairdneri Rich.) immunoglobulin specific serum from rabbits immunized with the slowest serum fraction isolated in polyacrylamide gel electrophoresis].
Rabbits were immunized with the slowest fraction obtained by polyacrylamide gel electrophoresis of normal Trout serum previously cleared of lipoproteins. The antiserum thus obtained reacts specifically with Trout immunoglobulin. This was verified by inhibiting the neutralization of Infectious Pancreatic Necrosis Virus by Trout antibodies, and by comparing it in immunoelectrophoresis with a rabbit antiserum previously obtained by immunization with Trout antibodies-Dinitrophenol-Haemocyanin immune complexes.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D000937:Antigen-Antibody Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera; D007136:Immunoglobulins / Q000302:isolation & purification*; D011817:Rabbits / Q000276:immunology; D012087:Reoviridae / Q000276:immunology; D012487:Salmonidae / Q000276:immunology*; D014337:Trout / Q000276:immunology*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007136:Immunoglobulins
Obtention d'un immunsérum spécifique anti-immunoglobuline de truite (Salmo gairdneri Rich.) chez des lapins immunisés à l'aide de la fraction du sérum sanguin la plus lente isolée par électrophorèse en gel de polyacrylamide.