Insulin secretion and (pro)insulin biosynthesis of cultured mice islets after glibenclamide loading in vitro.
Isolated islets from C57Bl/6J mice exposed to 10 mmoles/l glucose supplemented with 5 micrograms/ml glibenclamide for 48 h released significantly less insulin in the subsequent short-time incubation than untreated controls (without glibenclamide), whereas insulin biosynthesis was not suppressed by glibenclamide treatment.
D000818:Animals; D002478:Cells, Cultured; D005905:Glyburide / Q000494:pharmacology*; D007328:Insulin / Q000096:biosynthesis / Q000378:metabolism*; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D011384:Proinsulin / Q000096:biosynthesis*
D007328:Insulin; D011384:Proinsulin; D005905:Glyburide