[Factors increasing the effectiveness of Bacillus subtilis fusion].
The factors potentiating the efficacy of fusion of protoplasts of Bacillus subtilis were studied. A 50-fold increase in the yield of primary prototrophs was provided by 90-minute preincubation of protoplasts in a liquid hyperbaric medium and 10-minute treatment with trypsin. Increased efficacy of fusion of protoplasts depended on their regeneration percentage.
D000596:Amino Acids / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics*; D006824:Hybridization, Genetic; D006982:Hypertonic Solutions; D011523:Protoplasts / Q000187:drug effects / Q000502:physiology*; D014357:Trypsin / Q000494:pharmacology
D000596:Amino Acids; D006982:Hypertonic Solutions; D014357:Trypsin
Faktory, povyshaiushchie  éffektivnost' sliianiia Bacillus subtilis.