The presence of a new 3-oxoacyl-CoA thiolase in rat liver peroxisomes.
A novel 3-oxacyl-CoA thiolase was found in rat liver. This thiolase, mitochondrial general 3-oxacyl-CoA thiolase and acetoacetyl-CoA thiolase were purified from the rat liver after the induction of these activities by the administration to rats of di(2-ethylhexyl)phthalate, which enhanced the peroxisomal beta-oxidation activity. The new 3-oxoacyl-CoA thiolase was distinguished from mitochondrial and cytoplasmic thiolases by the following: DEAE-cellulose chromatography, phosphocellulose chromatography, immunochemical titration, and substrate specificity. Subcellular fractionation of liver and sucrose-density-gradient centrifugation of the light mitochondrial fraction revealed that the new thiolase was mainly in peroxisomes.
D000102:Acetyl-CoA C-Acyltransferase / Q000302:isolation & purification / Q000378:metabolism*; D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D004790:Enzyme Induction; D007106:Immune Sera; D007118:Immunoassay; D005779:Immunodiffusion; D008099:Liver / Q000201:enzymology*; D008297:Male; D008830:Microbodies / Q000201:enzymology*; D009940:Organoids / Q000201:enzymology*; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology
D007106:Immune Sera; D000217:Acyltransferases; D000102:Acetyl-CoA C-Acyltransferase