[Isolation of all proteins from the 50S subparticles of ribosomes of Escherichia coli].
The paper proposes a method of preparative isolation of all proteins from the 50S subparticle of E. coli ribosomes. The method is based on (1) preliminary fractionation into protein groups and ribonucleoprotein particles by a consecutive treatment of the 50S particles with increasing LiCl concentrations, and (2) chromatographic separation of protein groups on DE- and CM-cellulose and gel-filtration of separate fractions. The method allows to obtain any protein required for studies in preparative amounts avoiding many chromatographic stages. A detailed scheme of isolation of all proteins is given together with quantitative data of yields of individual proteins calculated per 6 g of the 50S subparticles.
D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004926:Escherichia coli / Q000032:analysis*; D008970:Molecular Weight; D012261:Ribonucleoproteins / Q000302:isolation & purification; D012269:Ribosomal Proteins / Q000302:isolation & purification*; D012270:Ribosomes / Q000032:analysis
D012261:Ribonucleoproteins; D012269:Ribosomal Proteins
Vydelenie vsekh belkov iz 50S chastits ribosom Escherichia coli.