Comparison of biologic potency of human insulin (recombinant DNA) and purified porcine insulin (PPI) on the rat and human adipocyte lipogenesis model.
To assess whether human insulin (recombinant DNA) is superior to porcine insulin, we compared the biologic effect of these two insulin preparations on rat and human adipocyte lipogenesis in vitro. The biologic potency of these two insulin preparations was similar on both human and rat adipocytes. Iodination of human and porcine insulin with 125I at A14 position led to a significant but similar loss of potency of both insulin preparations.
D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D001681:Biological Assay; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D008055:Lipids / Q000096:biosynthesis*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000494:pharmacology; D013552:Swine
D007328:Insulin; D008055:Lipids; D011994:Recombinant Proteins