Pyruvate dehydrogenase activity in group N streptococci.
Pyruvate dehydrogenase activity was detected in whole cells but not in cell-free extracts of Streptococcus lactis. However, the three component enzymes (pyruvate decarboxylase, lipoate acetyltransferase and lipoyl dehydrogenase) of the pyruvate dehydrogenase complex were identified in the cell-free extracts. Whole cells of the three species of group N streptococci formed acetoin and diacetyl only after the pathway forming acetate had become saturated. S. lactis subsp. diacetylactis DRC2 formed more acetoin and diacetyl and less acetate from pyruvate than did S. lactis C10. Strains C10 and DRC2 were able to form acetoin via alpha-acetolactate or diacetyl and to convert acetoin to butane-2,3-diol. S. cremoris HP was able to form acetoin only via alpha-acetolactate and could not convert acetoin to butane-2,3-diol.
D000123:Acetyltransferases / Q000378:metabolism; D000818:Animals; D001151:Arsenic / Q000494:pharmacology; D018053:Arsenites; D002245:Carbon Dioxide / Q000378:metabolism; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D004926:Escherichia coli / Q000201:enzymology; D013294:Lactococcus lactis / Q000201:enzymology*; D007785:Lactose / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism; D011767:Pyruvate Decarboxylase / Q000378:metabolism; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism; D017670:Sodium Compounds; D008063:Thioctic Acid / Q000031:analogs & derivatives / Q000378:metabolism
D018053:Arsenites; D011768:Pyruvate Dehydrogenase Complex; D011773:Pyruvates; D017670:Sodium Compounds; D002245:Carbon Dioxide; C017947:sodium arsenite; D008063:Thioctic Acid; D008058:Dihydrolipoamide Dehydrogenase; D000123:Acetyltransferases; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D011767:Pyruvate Decarboxylase; D007785:Lactose; D001151:Arsenic; D010100:Oxygen