High-performance liquid chromatographic and gas-liquid chromatographic determination of diazepam and nordiazepam in plasma.
A one-step method for extraction of diazepam, nordiazepam, and internal standard into toluene is followed by chromatographic separation and detection with either dual-wave-length high-performance liquid chromatography or electron-capture gas-liquid chromatography. Agreement between the two methods was excellent for diazepam (r = 0.99, n = 38) and good for nordiazepam (r = 0.96, n = 79) over a concentration range that included subtherapeutic, therapeutic, and toxic plasma levels.
D000328:Adult; D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003975:Diazepam / Q000031:analogs & derivatives* / Q000097:blood* / Q000627:therapeutic use; D006801:Humans; D008297:Male; D003708:Nordazepam / Q000097:blood*; D013226:Status Epilepticus / Q000097:blood / Q000188:drug therapy
D003708:Nordazepam; D003975:Diazepam