Headspace analysis of volatile metabolites of Pseudomonas aeruginosa and related species by gas chromatography-mass spectrometry.
Gas chromatographic-mass spectrometric analysis of headspace volatiles was performed on cultures of 11 strains of Pseudomonas aeruginosa and 1 strain each of Pseudomonas cepacia, Pseudomonas putida, Pseudomonas putrefaciens, Pseudomonas fluorescens, and Pseudomonas maltophilia. All strains of Pseudomonas aeruginosa produced a distinctive series of odd-carbon methyl ketones, particularly 2-nonanone and 2-undecanone, and 2-aminoacetophenone. The other strains failed to produce 2-aminoacetophenone. Two sulfur compounds, dimethyldisulfide and dimethyltrisulfide, were present in strains of P. aeruginosa and in variable amounts in other species. Butanol, 2-butanone, 1-undecene, and isopentanol were also detected in P. aeruginosa cultures.
D000438:Alcohols / Q000096:biosynthesis; D001431:Bacteriological Techniques; D002849:Chromatography, Gas; D007659:Ketones / Q000096:biosynthesis*; D013058:Mass Spectrometry; D011549:Pseudomonas / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000378:metabolism*; D013440:Sulfides / Q000096:biosynthesis*
D000438:Alcohols; D007659:Ketones; D013440:Sulfides