The asparagine-linked sugar chains of the glycoproteins in calf thymocyte plasma membrane. Structural studies of neutral oligosaccharides.
The neutral oligosaccharide fraction obtained from the hydrazinolysate of the plasma membrane glycoproteins of calf thymocytes was shown to be a mixture of twelve oligosaccharides. Oligosaccharides A, B, and C, which released galactose on treatment with jack bean beta-galactosidase but not with diplococcal beta-galactosidase, were shown to have the structures Gal beta 1-3Gal beta 1-4GlcNAc beta 1-4(Gal beta 1-3Gal beta 1-4GlcNAc beta 1-2)Man alpha 1-6(3)[Gal beta 1-3Gal beta 1-4GlcNAc beta 1-6(Gal beta 1-3Gal beta 1-4GlcNAc beta 1-2)Man alpha 1-3(6)]Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-6)GlcNAc, Gal beta 1-3Gal beta 1-4GlcNAc beta 1-4(Gal beta 1-3Gal beta 1-4GlcNAc beta 1-2)Man alpha 1-6(3)[Gal beta 1-3Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3(6)]Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-6)GlcNAc, and Gal beta 1-3Gal beta 1-4GlcNAc beta 1-2Man alpha 1-6(Gal beta 1-3Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-6)GlcNAc, respectively. Oligo saccharides D and E, which released galactose on treatment with either jack bean beta-galactosidase or diplococcal beta-galactosidase, were shown to have the structures Gal beta 1-4GlcNAc beta 1-2Man alpha 1-(Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-)GlcNAc and Gal beta 1-4GlcNAc beta 1-2Man alpha 1-(GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-)GlcNAc, respectively. Five oligosaccharides (F1, G, H1, I, and J) were shown to have typical high-mannose type structures (Man alpha 1-)n.Man beta 1-4GlcNAc beta 1-GlcNAc, with n-values of 8, 7, 6, 5, and 4, respectively. The structures of the remaining two oligosaccharides F2 and H2 were elucidated as GlcNAc beta 1-2Man alpha 1-(GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-)GlcNAc and Man alpha 1-(Man alpha 1-)Man beta 1-(GlcNAc beta 1-Man alpha 1-)Man beta 1-GlcNAc beta 1-GlcNAc, respectively.
D000818:Animals; D001216:Asparagine; D002240:Carbohydrate Sequence; D002417:Cattle; D002462:Cell Membrane / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D006023:Glycoproteins / Q000032:analysis*; D008361:Mannosidases; D009844:Oligosaccharides / Q000032:analysis*; D010944:Plants / Q000201:enzymology; D013296:Streptococcus pneumoniae / Q000201:enzymology; D013950:Thymus Gland / Q000032:analysis*; D001616:beta-Galactosidase
D006023:Glycoproteins; D009844:Oligosaccharides; D001216:Asparagine; D008361:Mannosidases; D001616:beta-Galactosidase