Mechanism of pBR322 transduction mediated by cytosine-substituting T4 bacteriophage.
A cytosine-substitution type mutant of bacteriophage T4 (T4dC phage) has been shown to mediate the transfer of plasmid pBR322. The transduction frequency was around 10(-2) per singly infected cell at low multiplicity of infection. The transductants contained either a monomer or multimers of pBR322. The transducing capacity of T4dC phage was resistant to methylmethanesulfonate treatment. The results of Southern blotting experiments have indicated that the pBR322 DNA exists as head-to-tail concatemers in the transducing particles. The mechanism of transfer of pBR322 mediated by T4dC phages is discussed.
D003596:Cytosine; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D013604:T-Phages / Q000235:genetics*; D014161:Transduction, Genetic
D003596:Cytosine