[Preparative separation of the tryptic hydrolysate of a protein by high-pressure liquid chromatography. The primary structure of a monoclonal L-chain of k-type, subgroup I (Bence-Jones Protein Wes) (author's transl)].
The tryptic hydrolysate of Bence-Jones protein Wes (Mr = 23000) was separated by high-pressure liquid chromatography in a volatile buffer system on a reversed-phase column. From the resulting 22 peptides 18 yielded integer numbers after amino acid analysis. They could be used directly for a modified Edman degradation. Two peptides were not separated by this procedure, two were missing. Under different conditions these four peptides could also be prepared in pure form. 3 mg was sufficient to elucidate the primary structure of the variable part of this protein completely. The arrangement of the tryptic peptides was deduced by homology to other k=chains. Protein Wes contains 214 residues and belongs to subgroup I of the k-chains. The valine residue in position 191 indicates that it belongs to allotype Inv b+.
D001536:Bence Jones Protein; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002918:Chymotrypsin; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D046911:Macromolecular Substances; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D014357:Trypsin
D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D046911:Macromolecular Substances; D010446:Peptide Fragments; D001536:Bence Jones Protein; D002918:Chymotrypsin; D014357:Trypsin
Präparative Auftrennung des tryptischen Hydrolysats eines Proteins mit Hilfe der hochdruck-Flüssigkeitschromatographie. Die Primärstruktur einer monoklonalen L-Kette vom K-Typ, Subgruppe I (Bence-Jones-Protein Wes).