[Preparation of immunosorbents from lipopolysaccharides and polysaccharides extracted from various gram-negative and gram-positive bacteria].
The method of binding of lipopolysaccharides (LPS) extracted from Salmonella typhimurium to aminohexyl-sepharose 4B by activation with benzoquinone was applied to three different LPS extracted from several enterobacteria species: S. seftenberg 1,3,19, S. cholerae suis 6(2),7 and Escherichia coli O141:H32. It was also used for two polysaccharides (PS) extracted from S. seftenberg 1,3,19 and Streptococcus agalactiae type II strain, respectively. Both PS were free from amino groups but exhibited the corresponding antigenic determinants of the cell wall. The use of these immunosorbents enabled us to obtain a monospecific antiserum. They may be a useful tool for serological identification of salmonella and group B streptococci. This method may be applied for other bacterial surface PS. The possible regeneration of such immunosorbents without appreciable loss of their antigen binding capacity makes possible their use for obtaining monospecific antibodies on a preparative scale.
D001666:Binding Sites, Antibody; D004755:Enterobacteriaceae / Q000032:analysis / Q000276:immunology*; D004926:Escherichia coli / Q000032:analysis / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D007163:Immunosorbent Techniques; D008070:Lipopolysaccharides / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D012475:Salmonella / Q000032:analysis / Q000276:immunology; D013292:Streptococcus agalactiae / Q000032:analysis / Q000276:immunology*
D007106:Immune Sera; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial
Préparation d'immunoabsorbants à partir de lipopolyosides et de polyosides extraits de différentes bactéries à gram négatif et à gram positif.