The cytotoxic action of activated and non-activated cyclophosphamide in yeast: comparison of induced DNA damage.
The cytotoxic and DNA-damaging effects of cyclophosphamide (CP) and its 'activated' derivative 4-OOH-CP were studied using a series of strains of S. cerevisiae which allow a phenotypical classification of genotoxic characteristics as well as direct physicochemical demonstration of key DNA lesions. The concurring results of biological and biochemical experiments indicate that (i) non-activated CP has a weak but detectable monofunctional alkylating potency, leading to DNA strand breaks and (ii) 4-OOH-CP has the ability to induce both DNA strand breaks and interstrand cross-links. The activity of CP is probably due to spontaneous decomposition in aqueous solution.
D000478:Alkylation; D003520:Cyclophosphamide / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004260:DNA Repair / Q000187:drug effects*; D004277:DNA, Single-Stranded; D012441:Saccharomyces cerevisiae / Q000187:drug effects*
D004277:DNA, Single-Stranded; C012358:4-hydroxycyclophosphamide; D003520:Cyclophosphamide