Antagonistic action between antibodies directed against 7-methylguanosine and polyamines on translation in vitro of RNA induced by measles virus.
Antibodies directed against N7-methylguanosine (m7Guo) were prepared and added to a wheat germ cell-free protein-synthesizing system programmed with RNA extracted from monkey cells persistently infected with measles virus. A dose-dependent inhibition of [35S]methionine incorporation was observed when RNA was preincubated with anti-m7Guo immunoglobulins. Antibodies preincubated with m7Guo did not show any inhibiting activity. The inhibitory effect of antibodies was abolished when RNA was preincubated with immunoglobulins in the presence of spermine and spermidine. When polyamines were added to the assay programmed with the IgG-RNA complex, no inhibition was observed.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D002460:Cell Line; D002472:Cell Transformation, Viral; D002474:Cell-Free System; D006151:Guanosine / Q000031:analogs & derivatives* / Q000276:immunology / Q000494:pharmacology; D000882:Haplorhini; D007700:Kinetics; D008459:Measles virus / Q000187:drug effects / Q000378:metabolism*; D011073:Polyamines / Q000276:immunology / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D012367:RNA, Viral / Q000378:metabolism*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D011073:Polyamines; D012367:RNA, Viral; D006151:Guanosine; C016578:7-methylguanosine