A simple method for detecting cells producing antibodies of specific immunoglobulin classes in the chicken.
A simple plaque method for detecting cells producing antibodies of specific immunoglobulin class in the chicken is described. This method is based upon the inability of chicken antibody to activate guinea-pig complement. IgM- and IgG-specific plaque-forming cells in the spleen of chickens immunized with sheep red blood cells were detected using guinea-pig complement and rabbit anti-chicken-mu- or gamma-chain serum. The specificity of the immunoglobulin class of plaques was confirmed by the abolition of class-specific hemolytic plaques after treatment of the lymphoid cells with rabbit antisera specific for chicken heavy chains in the presence of guinea-pig complement. The method is very simple and rapid, and IgM and IgG specific plaques are reliably detected.
D000818:Animals; D000921:Antibody-Producing Cells; D002645:Chickens; D003165:Complement System Proteins; D003602:Cytotoxicity, Immunologic; D006168:Guinea Pigs; D006462:Hemolytic Plaque Technique; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007142:Immunoglobulin gamma-Chains; D007148:Immunoglobulin mu-Chains; D007136:Immunoglobulins / Q000145:classification*; D011817:Rabbits; D012756:Sheep
D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007142:Immunoglobulin gamma-Chains; D007148:Immunoglobulin mu-Chains; D007136:Immunoglobulins; D003165:Complement System Proteins