Monoclonal antibodies to influenza matrix protein: detection of low levels of matrix protein on abortively infected cells.
We have quantified the appearance of matrix (M-) protein on P815 mastocytoma cells infected with type A influenza virus using a monoclonal antibody specific for M-protein. In contrast to previously reported values, only low amounts (about 10(3) molecules/cell) of M-protein appear on the cell surface up to 16 h p.i. Since P815 cells are excellent targets for cross-reactive T-cell lysis 3 to 5 h after virus infection, when only about 10(2) M-protein sites are found on each cell surface. It appears less likely that the recognition of M-protein can account for the cross-reactivity of cytotoxic T-lymphocytes for different type A influenza viruses.
D000818:Animals; D000914:Antibodies, Viral; D000937:Antigen-Antibody Reactions; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology*; D009980:Influenza A virus / Q000254:growth & development / Q000276:immunology*; D012515:Mast-Cell Sarcoma; D013997:Time Factors; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014764:Viral Proteins