Immunohistochemical identification of factor X-like antigen in the A cells of the normal human pancreas.
An indirect immunofluorescence technique using two rabbit anti-human factor X sera was used to investigate the presence of factor X in various human tissues. Of all tissues studied only the cytoplasm of some islet cells of the six human pancreases illustrated specific reactivity for factor X-like material. No reaction was seen in the exocrine pancreas. Cross-absorption studies with factor X-deficient plasma, glucagon or alpha-1-antitrypsin did not modify the pattern. On the contrary, cross-absorption with factor X Friuli or with normal plasma eliminated the positivity. No factor X-like material was seen in rat or guinea pig pancreas. The double-immunofluorescence technique demonstrated that the reactivity was localized only in the cytoplasm of the A cells.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D005170:Factor X / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006801:Humans; D007515:Islets of Langerhans / Q000276:immunology*; D051381:Rats
D000941:Antigens; D005170:Factor X