An enzymatically driven membrane reconstitution from solubilized components.
Acyltransferase activity is present in a variety of membranes species, including liver microsomes. The substrates of this enzyme are lysophosphatides and acyl CoA derivatives. We have found that the detergent effect of these substrates can be used to solubilize rat liver microsomes. If the solubilized fraction in incubated, the acyltransferase acylates the lysophosphatide and thereby degrades the detergent effect so that vesicular membranes re-form. Gel electrophoresis patterns show that the reconstituted membranes contain all of the major protein components of the original microsomes. A marker enzyme for liver microsomes, NADPH-cytochrome c reductase, was present in the reconstituted membranes at 70% of the specific activity in the original microsomes, and freeze-fracture electron microscopy showed intramembrane particles on all fracture faces. The system may provide a useful model for studies particles on all fracture faces. This system may provide a useful model for studies of certain membrane biogenesis reactions that utilize acyltransferase in vivo.
D000214:Acyl Coenzyme A / Q000378:metabolism*; D000215:Acylation; D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D002474:Cell-Free System; D005614:Freeze Fracturing; D008244:Lysophosphatidylcholines / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D009829:Oleic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D051381:Rats
D000214:Acyl Coenzyme A; D008244:Lysophosphatidylcholines; D009829:Oleic Acids; D010713:Phosphatidylcholines; C017585:oleoyl-coenzyme A; D009251:NADPH-Ferrihemoprotein Reductase; D000217:Acyltransferases