Daily variations in the activity of beta-N-acetylglucosaminidase in fractions of heart homogenates from male mice.
Daily variations of beta-N-acetylglucosaminidase activity in mouse myocardium were investigated. The maximal activity of the enzyme occurred at 8.00 p.m., minimal at 12.00 a.m. Whole body irradiation with X-rays (2100 r, 25 mA, 50 kV) brought about an increase of the activity and a shift of its maximum to 12.00 p.m. The determination of enzyme activity in the sediment and supernatant fractions allowed to calculate enzyme latency, as well as to draw conclusions concerning possible defects of the lysosomal membranes, the biosynthesis of the enzyme and its distribution between the sedimenting and non-sedimenting fraction.
D000118:Acetylglucosaminidase / Q000378:metabolism* / Q000528:radiation effects; D000818:Animals; D002940:Circadian Rhythm / Q000528:radiation effects; D006321:Heart / Q000528:radiation effects*; D006596:Hexosaminidases / Q000378:metabolism*; D008297:Male; D051379:Mice; D009206:Myocardium / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology; D014916:Whole-Body Irradiation
D006596:Hexosaminidases; D000118:Acetylglucosaminidase