Carcinogenic response of rainbow trout (Salmo gairdneri) to aflatoxin Q1 and synergistic effect of cyclopropenoid fatty acids.
Aflatoxin Q1 (AFQ1), the major microsomal biotransformation product of aflatoxin B1 (AFB1) formed in vitro by monkey and human liver preparations, was fed to rainbow trout (Salmo gairdneri) in a semipurified diet at levels of 20 ppb for 1 year and 100 ppb for 10 months. As a test for carcinogenicity in hatched fish, it was also used at 1 ppm in a water solution and exposed for 1 hour to fertile trout eggs. The 20-ppb dietary exposure and 1-ppm egg exposure failed to elicit a carcinogenic response; however, the 100-ppb dietary exposure produced a 10.6% incidence of hepatocellular carcinomas at the end of 1 year. Administration of 50 ppm methyl sterculate, a cyclopropenoid fatty acid, in combination with 100 ppb AFQ1 resulted in a synergistic tumor response similar to that previously noted with administration of AFB1 plus aflatoxin M1. The tumor incidence was 89.1% in the fish on the combined diet. These results indicated that AFQ1 was approximately 100 times less carcinogenic than was AFB1. This comparison of carcinogenic potencies was very similar to the comparison of the relative mutagenicities of the two compounds in the Ames bacterial mutagen assay system.
D000348:Aflatoxins / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D001711:Biotransformation; D003043:Cocarcinogenesis; D003521:Cyclopropanes / Q000494:pharmacology*; D004357:Drug Synergism; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D000882:Haplorhini; D066298:In Vitro Techniques; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced* / Q000473:pathology; D008862:Microsomes, Liver / Q000378:metabolism; D014337:Trout
D000348:Aflatoxins; D003521:Cyclopropanes; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; C023396:methyl sterculate