Hemagglutination antigen of Mycobacterium tuberculosis. Immunochemical characterization and concanavalin A reactivity.
The hemagglutination antigen isolated from Mycobacterium tuberculosis strain Aoyama B was characterized by immunoelectrophoresis using the International Reference System for Mycobacterial Antigens. It showed two precipitin bands, the major one being identical with antigen 1 (arabinomannan). The minor precipitin band formed a spur with antigen 2 (arabinogalactan), attesting to the presence of common determinant groups. The immunoelectrophoretic analysis of the hemagglutination antigen against concanavalin A also revealed 2 precipitin bands, the major one being identical with the single precipitin band formed between the reference antigen and concanavalin A. The 2 arabinomannans present seemed to differ in molecular weight.
D003208:Concanavalin A / Q000276:immunology; D006384:Hemagglutination; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008070:Lipopolysaccharides / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011233:Precipitin Tests
D008070:Lipopolysaccharides; D003208:Concanavalin A