Use of isotope effects to elucidate enzyme mechanisms.
The chemical bond breaking steps are normally not rate limiting for enzymatic reactions. However, comparison of deuterium and tritium isotope effects on the same reaction, especially when coupled with 13C isotope effects for the same step measured with deuterated as well as unlabeled substrates, allows calculation of the intrinsic isotope effects on the bond breaking steps and thus a determination of the commitments to catalysis for the reactants. The variation in observed isotope effects as a function of reactant concentration can be used to determine kinetic mechanisms, while the pH variation of isotope effects can determine the stickiness of the reactants and which portions of the reactant mechanism are pH dependent. Finally the size of primary and secondary intrinsic isotope effects can be used to determine transition state structure.
D000818:Animals; D003903:Deuterium / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D005649:Fumarate Hydratase / Q000378:metabolism; D007554:Isotopes / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D008722:Methods; D008956:Models, Chemical
D004798:Enzymes; D007554:Isotopes; D003903:Deuterium; D005649:Fumarate Hydratase