Biosynthesis of polymyxin E by a cell-free enzyme system. II. Synthesis of enzyme-bound octanoyldiaminobutyric acid.
A partially purified enzyme of Aerobacillus polyaerogenes, which produces polymyxin E, activates L-2,4-diaminobutyric acid and binds it as a thioester. The incubation of the enzyme preparation with octanoyl coenzyme A and L-2,4-diaminobutyric acid in the presence of ATP and an ammonium sulfate fraction yields octanoyldiaminobutyric acid thioesterified to the enzyme.
D000613:Aminobutyrates / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology*; D002854:Chromatography, Paper; D003091:Colistin / Q000096:biosynthesis*; D007930:Leucine; D011485:Protein Binding; D013912:Threonine
D000613:Aminobutyrates; D013912:Threonine; C005959:2,4-diaminobutyric acid; D007930:Leucine; D003091:Colistin