In vitro susceptibility of Pseudomonas aeruginosa to carbenicillin, glycine, and ethylenediaminetetraacetic acid combinations.
Striking bacterial activity against Pseudomonas aeruginosa 9D-2 was achieved by glycine-carbenicillin, ethylenediaminetetraacetic acid-carbenicillin, and glycine-ethylenediaminetetraacetic acid combinations, whereas none of the agents used alone was capable of the same degree of bactericidal activity. Studies using a microtiter modification of the checkerboard technique were performed to evaluate the comparative activity of these antimicrobial combinations. Isobolograms showed synergistic effects with carbenicillin-glycine, carbenicillin-ethylene-diaminetetraacetic acid, and glycine-ethylenediaminetetraacetic acid combinations. Bacterial growth inhibitory curves with subinhibitory concentrations of these agents in combination confirmed these findings.
D002228:Carbenicillin / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D004357:Drug Synergism; D004492:Edetic Acid / Q000494:pharmacology*; D005998:Glycine / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010457:Peptidoglycan / Q000037:antagonists & inhibitors; D011550:Pseudomonas aeruginosa / Q000187:drug effects*
D010457:Peptidoglycan; D004492:Edetic Acid; D002228:Carbenicillin; D005998:Glycine