Evidence for the presence of the putative TRH metabolite, deamido-TRH, in human urine.
Using a specific radioimmunoassay (RIA) for deamido TRH (TRH-OH), TRH-OH immunoreactivity (IR) was detected in the urine of 54% of a group of normal subjects and patients with thyroid disease. Human urine TRH-OH IR eluted in the position of synthetic TRH-OH on Sephadex G-10 gel filtration (GF). In contrast, urine TRH IR, as determined by a specific TRH RIA, did not elute with synthetic TRH. HU-TRH was prepared for studies on its crossreactivity in the TRH-OH RIA. HU-TRH is the term given to a previously described substance(s) which is obtained from human urine by immunoabsorption to anti-TRH antiserum, is reactive in the TRH RIA, and shows non-identity with synthetic TRH on GF. HU-TRH was not reactive in the TRH-OH RIA. These studies suggest that the putative TRH metabolite, deamido TRH, is present in human urine and that HU-TRH is neither TRH-OH or as closely related structurally to TRH-OH as it is to TRH.
D002850:Chromatography, Gel; D006801:Humans; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D011863:Radioimmunoassay; D012016:Reference Values; D013959:Thyroid Diseases / Q000652:urine; D013973:Thyrotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000652:urine
C020271:thyrotropin-releasing hormone, deamido-; D013973:Thyrotropin-Releasing Hormone; D011761:Pyrrolidonecarboxylic Acid