Isolation and amino acid sequence of urotensin I, a vasoactive and ACTH-releasing neuropeptide, from the carp (Cyprinus carpio) urophysis.
Urotensin I (UI), a 41-residue mammalian hypotensive and fish or mammalian corticotropin-releasing peptide, isolated from 0.1 N HCI extracts of urophyses of the carp (Cyprinus carpio) was purified and the amino acid sequence was determined to be: H-Asn-Asp-Asp-Pro-Pro-Ile-Ser-Ile-Asp-Leu-Thr-Phe-His-Leu-Leu- Arg-Asn-Met-Ile-Glu-Met-Ala-Arg-Asn-Glu-Asn-Gln-Arg-Glu-Gln-Ala-Gly-Leu-Asn-Arg-Lys-Tyr-Leu-Asp-Glu-Val-NH2. When the extraction procedure included heating at 100 degrees C for 15 min, UI was cleaved at a highly acid labile Asp-Pro bond to give the fully active UI (4-41). Urotensin I shows close structural and biological homology with the recently isolated ovine hypothalamic corticotropin-releasing factor (CRF) and the frog skin peptide sauvagine and thus may be considered an evolutionary prototype of unique mammalian-hypotensive and vertebrate corticotropin-releasing factors.
D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D002347:Carps; D010450:Endopeptidases; D009490:Neurosecretory Systems / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides / Q000302:isolation & purification*; D051381:Rats; D014579:Urotensins / Q000302:isolation & purification* / Q000494:pharmacology
D010446:Peptide Fragments; D010455:Peptides; D014579:Urotensins; C029676:urotensin I; D010450:Endopeptidases