The effect of anti-immunoglobulin antibodies on the in vitro proliferation and differentiation of normal and neoplastic murineB cells.
In these studies, we have compared the effects of anti-mu and anti-delta antibodies on normal and neoplastic murine B cells. Spleen cells from normal BALB/c mice or from mice carrying the BCL1 tumor were treated with F(ab')2 fragments of affinity purified anti-mu or anti-delta antibodies and the effec of the antibodies on cell proliferation was assessed. F(ab)'2 fragments of both antibodies induced proliferation of normal adult B cells but inhibited (anti-mu) or had no effect (anti-delta) on proliferation of BCL1 cells. In addition, both antibodies further enhanced the LPS-induced proliferative response of cells from adult mice. In contrast, the LPS responses of neonatal cells and BCL1 cells were inhibited by F(ab')2 anti-mu antibodies. These data indicate that interaction of antibody with surface IgD as well as surface IgM can activate adult B cells. In addition, the results provide further evidence that BCL1 cells are neoplastic analogs of immature B cells since the tumor cells, like neonatal cells, are not activated after treatment with anti-Ig antibodies.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000921:Antibody-Producing Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology*; D002454:Cell Differentiation; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D007075:Immunoglobulin M / Q000096:biosynthesis; D007148:Immunoglobulin mu-Chains; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D013154:Spleen / Q000276:immunology; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D000888:Antibodies, Anti-Idiotypic; D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains; D013936:Thymidine