Cultivation in vitro of the quartan malaria parasite Plasmodium inui.
The simian guartan malaria parasite Plasmodium inui (OS strain) was cultured in a continuous flow system with rhesus monkey erythrocytes and RPMI 1640nmedium supplemented with Hepes buffer and rhesus serum. Over a 10-week period, the growth of the parasite permitted a 61,000-fold cumulative dilution of the original inoculum. After 5 weeks in culture, the parasites were still infective to the monkey Saimiri sciureus and to Anopheles freeborni mosquitoes.
D000818:Animals; D002478:Cells, Cultured; D004912:Erythrocytes / Q000469:parasitology*; D000882:Haplorhini / Q000469:parasitology*; D007814:Larva; D008251:Macaca / Q000469:parasitology*; D010961:Plasmodium / Q000166:cytology / Q000254:growth & development*