Effect of detergents and acid buffer on the reactivity of Fasciola hepatica antigens.
The effect of glycine-HCl (acid) buffer on antigen extraction and of three commonly used detergents--sodium dodecyl sulphate (SDS), sodium deoxycholate (DOC), and Triton X-100--on the reactivity of a standard Fasciola hepatica adult-worm antigen preparation (FhWWE) was studied. Acid buffer destroyed most of the immunologic reactivity of FhWWE when tested with anti-F. hepatica serum, but apparently did not affect the antigens reactivity with an anti-S. mansoni serum. These studies suggest that elution with acid buffer should be used with caution when FhWWE is the antigenic extract used in antibody-affinity chromatography. Neither Triton X-100 nor DOC appreciably increased the reactivity of FhWWE antigens. In addition, DOC decreased the antigen-antibody interactions. Low concentrations of SDS increased the reactivity of FhWWE, but higher concentrations decreased the antigen-antibody interactions.
D000818:Animals; D000941:Antigens / Q000276:immunology*; D002021:Buffers; D003840:Deoxycholic Acid / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology*; D005210:Fasciola hepatica / Q000276:immunology*; D005998:Glycine / Q000494:pharmacology*; D006851:Hydrochloric Acid / Q000494:pharmacology*; D005779:Immunodiffusion; D011092:Polyethylene Glycols / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D000941:Antigens; D002021:Buffers; D003902:Detergents; D003840:Deoxycholic Acid; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D006851:Hydrochloric Acid; D005998:Glycine