Purification and immunochemical analysis of histidine decarboxylase from murine mastocytoma.
Application of a new scheme of purification for histidine decarboxylase (E.C. 4.1.22) leads to a highly purified enzyme (5000 nmol/mg . h, i.p. 5.0, M.2.: 110 kD) with reasonable stability (rest activity 80% after 3 weeks at 6--8 degrees C). A major protein contaminant seen on electrofocusing (i.p. 4.6) shows immunological identity with the enzyme-containing protein (i.p. 5.0) and might be involved in the aggregation of the enzyme.
D000818:Animals; D002262:Carboxy-Lyases / Q000302:isolation & purification*; D006640:Histidine Decarboxylase / Q000302:isolation & purification*; D007120:Immunochemistry; D012515:Mast-Cell Sarcoma / Q000201:enzymology; D051379:Mice; D012513:Sarcoma, Experimental / Q000201:enzymology*
D002262:Carboxy-Lyases; D006640:Histidine Decarboxylase