A comparison of immunoferritin, immuno-enzyme and gold-labelled protein A methods for the localization of capsular antigen on frozen thin sections of the bacterium, Pasteurella haemolytica.
The staphylococcal protein A-gold method was found to be superior to the enzyme-or ferritin-linked antibody techniques for locating capsular antigens on cryosections of Pasteurella haemolytica, and its sensitivity was similar to the enzyme-linked antibody method. The sensitivity of conventionally fixed and embedded material and cryosections of heavily fixed, lightly fixed and unfixed material were shown to be similar under routine laboratory conditions.
D000942:Antigens, Bacterial / Q000032:analysis*; D003102:Colloids; D005293:Ferritins; D005629:Frozen Sections; D006046:Gold; D007124:Immunoenzyme Techniques; D010325:Pasteurella / Q000276:immunology*; D013205:Staphylococcal Protein A
D000942:Antigens, Bacterial; D003102:Colloids; D013205:Staphylococcal Protein A; D006046:Gold; D005293:Ferritins