Membrane filtration of dairy products for microbiological analysis.
Incubation with protease or Tween 80 or both dramatically improved the membrane filterability of liquid milks, powdered skim milk, and frozen dairy products without reducing the viability of five common species of bacteria. The technique can permit isolation and enumeration of microorganisms from test samples of these foods as large as 5 g. Flow direction through the filter was an important factor in filterability of dairy products.
D000818:Animals; D002265:Carboxylic Ester Hydrolases; D002417:Cattle; D003611:Dairy Products; D013293:Enterococcus faecalis / Q000302:isolation & purification; D004926:Escherichia coli / Q000302:isolation & purification; D005516:Food Microbiology; D005628:Frozen Foods; D008892:Milk; D010447:Peptide Hydrolases / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000302:isolation & purification; D014462:Ultrafiltration / Q000379:methods*
D011136:Polysorbates; D002265:Carboxylic Ester Hydrolases; C044495:tween 80 esterase; D010447:Peptide Hydrolases