Surface immunoglobulin, lectin-induced cap formation, and phagocytic function in five patients with the leukemic phase of hairy cell leukemia.
Hairy cells from 5 patients with greater than 25% hairy cells in the peripheral blood (4 had greater than 45% hairy cells and white blood cell counts (WBC) greater than 10,000/mm3) were studied for surface immunoglobulin (SIg) presence and distribution by two methods, for lectin-induced cap formation, and for phagocytosis of zymosan. Hairy cells from all 5 cases were found to have distinct monoclonal patterns, 2 Gk, 1 MDk, 1 Dk, and 1 GMDk, as well as cap formation with SIg. All 5 cases showed distinct lectin-induced cap formation in a percentage of cells similar to the percentage of hairy cells, and 2 of the 5 patients had hairy cells which phagocytosed zymosan. These findings contrasted with the malignant cells from 4 patients with CLL, which had monoclonal SIg but no SIg cap formation and no significant percentage of lectin-induced cap formation. Cells from 2 cases of T cell lymphomas had no SIg and no lectin-induced cap formation as did cells from 2 cases of non-lymphocytic leukemia. Hairy cells not only appear to have SIg cap formation similar to some B-lymphocytes, which in some patients also phagocytose zymosan, but also demonstrate strong lectin-induced cap formation.
D000328:Adult; D000368:Aged; D002462:Cell Membrane / Q000276:immunology; D005260:Female; D006801:Humans; D007142:Immunoglobulin gamma-Chains / Q000032:analysis; D007145:Immunoglobulin kappa-Chains / Q000032:analysis; D007152:Immunologic Capping; D037102:Lectins / Q000276:immunology; D007943:Leukemia, Hairy Cell / Q000276:immunology*; D007962:Leukocytes / Q000032:analysis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010587:Phagocytosis; D011947:Receptors, Antigen, B-Cell; D015054:Zymosan / Q000276:immunology
D007142:Immunoglobulin gamma-Chains; D007145:Immunoglobulin kappa-Chains; D037102:Lectins; D011947:Receptors, Antigen, B-Cell; D015054:Zymosan