Evaluation of 2 different genetic markers for the detection of frameshift and missense mutagens in A. nidulans.
21 chemicals, known to induce missense and/or frameshift mutations directly, were assayed for their ability to forward mutate a haploid strain of A. nidulans. 2 genetic markers for forward mutations were used, namely 8-azaguanine resistance and induction of meth A1 suppressors. Missense mutagens were usually active when tested with the plate-incorporation technique, whereas frameshift agents were ineffective; some of these, on the other hand, turned out to be positive when tested with a liquid-test procedure. The 2 genetic markers used showed a similar sensitivity (with only 2 exceptions) in detecting the chemical mutagens assayed.
D001233:Aspergillus nidulans / Q000187:drug effects / Q000235:genetics*; D001375:Azaguanine / Q000494:pharmacology; D004351:Drug Resistance; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D013489:Suppression, Genetic
D009153:Mutagens; D001375:Azaguanine