Regulation of calcium-binding protein messenger RNA by 1,25-dihydroxycholecalciferol.
1,25-Dihydroxycholecalciferol modulates the synthesis of the intestinal calcium-binding protein. To determine if this effect is due to an increase in calcium-binding protein mRNA activity, we measured total cytoplasmic protein-specific mRNA activity from chick intestine in an in vitro wheat germ translation system. 1,25-Dihydroxycholecalciferol enhances calcium-binding protein specific mRNA activity. The sterol does not induce a general increase in mRNA synthesis since the concentration of only a few proteins increased. Thus 1,25-dihydroxycholecalciferol regulates only a subset of genes and specifically affects a transcriptional process involving calcium-binding protein synthesis.
D000818:Animals; D002117:Calcitriol; D002135:Calcium-Binding Proteins / Q000096:biosynthesis*; D002645:Chickens; D004100:Dihydroxycholecalciferols / Q000494:pharmacology*; D004386:Duodenum / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006887:Hydroxycholecalciferols / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols; D012333:RNA, Messenger; D002117:Calcitriol