Solubilization and concentration of a bone-inducing substance from a murine osteosarcoma.
The osteogenic factor from murine osteosarcoma was soluble in 4M guanidine-HCl. The precipitate that appeared after dialysis of the guanidine solution against deionized water showed a higher osteogenetic activity than did the initial dry powder of tumor tissue. Electron-microscopically, this bone-inducing fraction seems to be homogenous. Neither a fibrous nor collagenous cross-banded structure was observed in the fraction. Since the chemical analysis of the fraction also showed a relatively low content of hydroxyproline, the osteogenic factor is probably not collagen.
D000818:Animals; D001859:Bone Neoplasms / Q000032:analysis* / Q000473:pathology; D051379:Mice; D009999:Ossification, Heterotopic / Q000473:pathology; D010012:Osteogenesis; D012516:Osteosarcoma / Q000032:analysis* / Q000473:pathology; D010455:Peptides / Q000032:analysis
D010455:Peptides