An improved apparatus for blood perfusion of the canine cerebral vasculature.
An improved perfusion apparatus is described which consists of a membrane oxygenator, roller pump, reservoir, heat exchanger, blood filter, and inert tubing. Heparinized blood may be used and is delivered at flow rates from 10 to 250 ml/min. Dogs are anesthetized with halothane and their cerebral arterial blood supply isolated by the method of Gilboe et al. (8). When the canine brain is perfused for 5 hr using the described apparatus, the rates of cerebral oxygen and glucose consumption are 5.19 +/- 0.12 mg/100 g/min and 39.9 +/- 6.5 mumol/100 g/min, respectively. Of the total glucose consumed by the brain, about 1/4 is contributed by the erythrocytes. An equivalent of about 9% of the consumed glucose is returned to the blood as lactate. Electron microscopic examination of cerebral cortex samples reveals no differences between 5-hr perfused brain and appropriate nonperfused controls. It is concluded that the apparatus is a useful system for organ perfusion and that the canine brain perfused by this method remains physiologically and metabolically active for at least 5 hr.
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001921:Brain / Q000502:physiology; D002245:Carbon Dioxide / Q000097:blood; D002560:Cerebrovascular Circulation; D004285:Dogs; D004569:Electroencephalography; D006400:Hematocrit; D010100:Oxygen / Q000097:blood; D010477:Perfusion / Q000295:instrumentation* / Q000379:methods
D001786:Blood Glucose; D002245:Carbon Dioxide; D010100:Oxygen