Properties of ablastin--a factor in the serum of rats infected with Trypanosoma lewisi which inhibits the parasites' division.
The present study investigated the nature of ablastin, a factor present in the serum of rats infected with Trypanosoma lewisi and which inhibits the parasites' division. Ablastin was unstable to dialysis at pH 1X8, was not adsorbed from serum by trypanosomes and could not be induced in vivo by means other than a natural infection. Attempts to purify ablastin from serum by conventional chromatographic techniques were unsuccessful. Removal of over 90% of the immunoglobulins from ablastinic serum did not reduce the ablastin titre. It is concluded that ablastin is unlikely to be an immunoglobulin as has been previously suggested.
D000818:Animals; D005260:Female; D006863:Hydrogen-Ion Concentration; D007114:Immunization; D007074:Immunoglobulin G / Q000302:isolation & purification / Q000494:pharmacology*; D007136:Immunoglobulins / Q000302:isolation & purification; D008297:Male; D051381:Rats; D014344:Trypanocidal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D014350:Trypanosoma lewisi / Q000187:drug effects*; D014352:Trypanosomiasis / Q000097:blood*
D007074:Immunoglobulin G; D007136:Immunoglobulins; D014344:Trypanocidal Agents; C019230:ablastin