The interaction of the K88 antigen with porcine intestinal epithelial cell brush borders.
The interaction of 125I-labelled K88 antigen with brush borders of the epithelial cells of the pig small intestine has been studied. The iodinated antigen bound avidly to the brush borders prepared from adhesive (receptor-positive) pigs even after pretreatment of the brush borders with formaldehyde, whereas the brush borders from non-adhesive (receptor-negative) pigs failed to bind the antigen under these conditions. Treatment with glutaraldehyde rapidly destroyed the ability of both types of brush border to bind the K88 antigen. Studies on the binding of antigen to brush borders revealed the presence of high affinity receptors, but the non-linearity of the Scatchard plot could be explained by cooperative-like interactions, which view was supported by dissociation experiments. Rapid dissociation only in the presence of unlabelled K88 antigen suggested the existence of receptor site interactions of the negatively cooperative type. Attempts to inhibit the binding of 125I-labelled K88 with simple monosaccharides and oligosaccharides suggested that the binding of antigen to brush borders involves complex interactions and that galactosyl residues may be important.
D000818:Animals; D000942:Antigens, Bacterial; D000954:Antigens, Surface / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology*; D004848:Epithelium / Q000648:ultrastructure; D004926:Escherichia coli / Q000276:immunology; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D005557:Formaldehyde / Q000494:pharmacology; D005697:Galactosides / Q000276:immunology; D005976:Glutaral / Q000494:pharmacology; D007421:Intestine, Small / Q000648:ultrastructure*; D008871:Microvilli / Q000276:immunology*; D011971:Receptors, Immunologic / Q000276:immunology*; D013552:Swine
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D029968:Escherichia coli Proteins; D005697:Galactosides; C026888:K88 antigen, E coli; D011971:Receptors, Immunologic; D036781:Fimbriae Proteins; D005557:Formaldehyde; D005976:Glutaral