A radiolabel release microassay for phagocytic killing of Candida albicans.
The chromium release technique for quantifying intracellular killing of radiolabelled Candida albicans particles was exploited in a microassay in which murine and human phagocytes acted as effectors under peculiarly simple conditions. At appropriate effector: target ratios and with a 4 h incubation, up to 50% specific chromium release could be detected in the supernatant with no need for opsonization or lysis of phagocytes. This simple microassay permits easy-to-perform, simultaneous testing of a variety of different phagocytes even if only available in limited amounts, and provides an objective measurement of intracellular killing of Candida albicans.
D000818:Animals; D002176:Candida albicans / Q000378:metabolism*; D002177:Candidiasis / Q000175:diagnosis; D002860:Chromium Radioisotopes; D003840:Deoxycholic Acid / Q000494:pharmacology; D003851:Deoxyribonucleases / Q000494:pharmacology; D005260:Female; D006801:Humans; D007158:Immunologic Techniques; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects; D011650:Pulmonary Alveoli / Q000166:cytology; D013154:Spleen / Q000166:cytology
D002860:Chromium Radioisotopes; D003840:Deoxycholic Acid; D003851:Deoxyribonucleases