Regulation of the anti-inulin antibody response by a nonallotype-linked gene.
The antibody response to the inulin [(In), beta-(2 leads to 1) fructosan] determinant of bacterial levan [(BL), a beta-(2 leads to 6) polyfructosan that contains beta-(2 leads to 1) branch points] requires the presence of the a haplotype of the Igh gene complex. BALB/c (Igh a) mice immunized with BL produce IgG anti-In antibodies of a single spectrotype by isoelectric focusing analysis. C57BL/6 mice, which possess the b haplotype of the Igh gene complex and which fail to produce anti-In antibodies, nevertheless possess a gene, spectrotype regulation gene 1 (Sr-1), that regulates the isoelectric focusing (IEF) pattern of anti-In antibodies in mice of the a haplotype. Thus, the IEF patterns of anti-In antibodies of (BALB/c x C57BL/6)F1 mice and of B.C8 mice (C57BL/Ka . Igh-Ca) are considerably more complex than those of BALB/c. Backcross analysis indicates that Sr-1 is not linked to the Igh complex, the major histocompatibility complex, or to the genes that code for coat color. Studies of the heterogeneity of anti-In antibodies in recombinant inbred lines and their progeny from matings to BALB/c and C.B20 (BALB/c . Igh-Cb) suggest the existence of other regulatory genes.
D000818:Animals; D000906:Antibodies / Q000235:genetics*; D005630:Fructans / Q000276:immunology*; D005802:Genes, MHC Class II; D005809:Genes, Regulator; D008040:Genetic Linkage; D007074:Immunoglobulin G / Q000235:genetics*; D007444:Inulin / Q000276:immunology*; D007526:Isoelectric Point; D008285:Major Histocompatibility Complex; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008810:Mice, Inbred C57BL / Q000276:immunology; D011134:Polysaccharides / Q000276:immunology*
D000906:Antibodies; D005630:Fructans; D007074:Immunoglobulin G; D011134:Polysaccharides; D007444:Inulin