Effects of 2-deoxy-D-glucose on the synthesis of RNA and protein in Saccharomyces carlsbergensis G-517.
When Saccharomyces carlsbergensis G-517 was grown in 10 mM galactose as the carbon source, the addition of 2-deoxy-D-glucose restricted the uptake of galactose, [3H]uridine and [3H]leucine, and restricted invertase synthesis (beta-D-fructofuranoside fructohydrolase; EC 3.2.1.26) for a period of 60-90 min. During this time, the radioactive antimetabolite was taken up by the cells; afterwards, invertase synthesis was enhanced, and the utilizaton rate of galactose, [3H]uridine and [3H]leucine increased until it reached that of the control culture. When glucose was used as a carbon source, sugar utilization and uptake of radioactive precursors were unaffected by addition of the deoxysugar.
D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D005656:Fungal Proteins / Q000096:biosynthesis*; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D015125:Oxyquinoline / Q000494:pharmacology; D012331:RNA, Fungal / Q000096:biosynthesis*; D012440:Saccharomyces / Q000187:drug effects / Q000378:metabolism*; D013393:Sucrase / Q000096:biosynthesis; D014529:Uridine / Q000378:metabolism
D003837:Deoxy Sugars; D005656:Fungal Proteins; D012331:RNA, Fungal; D015125:Oxyquinoline; D003847:Deoxyglucose; D013393:Sucrase; D007930:Leucine; D014529:Uridine