[Preparation of protoplast-like structures of Pseudomonas putida and their reversion to bacterial forms].
A technique is proposed for the preparation of Pseudomonas putida protoplast-like structures by treating the cells with lysozyme in a hypertonic medium containing mono-and divalent cations. Pretreatment of the cells at the logarithmic growth phase with sucrose (0.5 M) for 90 min at the pH 7.9 to 8.0 is a prerequisite for the formation of 'protoplasts'. Under these conditions, the yield of 'protoplasts' reached 99.9% of the total cell number. The protoplast-like structures are capable of reversing into the original bacterial forms in a minimal hypertonic medium containing amino acids which are components of the cell wall. The level of reversion reaches 10% for certain strains.
D001431:Bacteriological Techniques; D006982:Hypertonic Solutions; D009113:Muramidase / Q000494:pharmacology; D011523:Protoplasts / Q000187:drug effects*; D011549:Pseudomonas / Q000187:drug effects*; D013104:Spheroplasts / Q000187:drug effects / Q000302:isolation & purification
D006982:Hypertonic Solutions; D009113:Muramidase
Poluchenie protoplastopodobnykh struktur Pseudomonas putida i ikh reversiiia v bakterial'nye formy.