Mannitol estimation in biological fluids by gas-liquid chromatography of trimethylsilyl derivatives.
We describe a procedure for estimating mannitol concentrations in biological fluids. Samples are mixed with internal standard solution (alpha-methylglucose), deproteinized if necessary, desalted, and dried. Specimens are then derivatized by adding pyridine/bis(trimethylsilyl)acetamide/trimethylchlorosilane and heating at 60 degrees C for 30 min. Samples are chromatographed on a 275-cm column of 10% OV-17, operated at 190 degrees C, and quantitated by peak-height measurement. The technique is linear, accurate, precise, sensitive, and free from interference. It has been used to measure mannitol in plasma, urine, and bile.
D002849:Chromatography, Gas / Q000379:methods; D006601:Hexoses / Q000032:analysis; D006801:Humans; D008353:Mannitol / Q000032:analysis* / Q000652:urine; D013402:Sugar Alcohols / Q000032:analysis; D014297:Trimethylsilyl Compounds
D006601:Hexoses; D013402:Sugar Alcohols; D014297:Trimethylsilyl Compounds; D008353:Mannitol