The effect of Listeria monocytogenesis lipids on the mouse lymphocytes in vitro.
The effect of Listeria monocytogenes lipids (LML) on the incorporation of 3H-thymidine into mouse spleen lymphocytes cultured in vitro was studied. It has been found that LML was mitogenic for B lymphocytes but did not stimulate T lymphocytes. The presence of autologous T dependent cells did not change the intensity of B lymphocytes stimulation of LML. However, macrophages markedly increased the effect of LML on B lymphocytes. The supernatants of the cultures of unfractionated spleen lymphocytes stimulated with LML, contrary to those stimulated with PHA, did not inhibit the macrophage migration. It has been concluded that LML is an unspecific B lymphocyte mitogen.
D000276:Adjuvants, Immunologic; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D066298:In Vitro Techniques; D007553:Isotope Labeling; D008055:Lipids / Q000276:immunology*; D008089:Listeria monocytogenes / Q000276:immunology*; D008297:Male; D051379:Mice; D008934:Mitogens; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D013936:Thymidine; D014316:Tritium
D000276:Adjuvants, Immunologic; D008055:Lipids; D008934:Mitogens; D014316:Tritium; D013936:Thymidine