Specific inactivation of lysosomal glycosidases in living fibroblasts by the corresponding glycosylmethyl-p-nitrophenyltriazenes.
1. beta-D-Galactopyranosylmethyl-p-nitrophenyltriazene can specificag detectable changes in cell viability or the activities of other hydrolases. 2. beta-D-Glucopyranosylmethyl-p-nitrophenyltriazene behaves similarly towards lysosomal beta-glucosidase, but also inactivates some alpha-glucosidase. 3. Both beta-galactosidase and beta-glucosidase activities in triazene-treated confluent fibroblast cultures recover exponentially; if zero-order enzyme production is assumed, turnover times of 10 and 5 days respectively can be estimated.
D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology; D005696:Galactosidases / Q000037:antagonists & inhibitors*; D005697:Galactosides / Q000494:pharmacology*; D005959:Glucosidases / Q000037:antagonists & inhibitors*; D005960:Glucosides / Q000494:pharmacology*; D006027:Glycosides / Q000494:pharmacology*; D006801:Humans; D006867:Hydrolases / Q000037:antagonists & inhibitors; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology*; D011489:Protein Denaturation / Q000187:drug effects; D014226:Triazenes / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000037:antagonists & inhibitors*; D001617:beta-Glucosidase / Q000037:antagonists & inhibitors*
D005697:Galactosides; D005960:Glucosides; D006027:Glycosides; D014226:Triazenes; C018547:beta-D-galactopyranosylmethyl-4-nitrophenyltriazene; C025929:beta-D-glucopyranosyl-4-nitrophenyltriazene; D006867:Hydrolases; D005696:Galactosidases; D005959:Glucosidases; D001617:beta-Glucosidase; D001616:beta-Galactosidase