Fluorescence relaxation of proflavin-deoxyribonucleic acid interaction. Kinetic properties of a base-specific reaction.
The kinetics of proflavin binding to Micrococcus lysodeicticus deoxyribonucleic acid (DNA [(G-C) content 72%], to Bacillus megaterium DNA [(A-T) content 70%], and to the polydeoxyribonucleotides poly[d(G-C)] and poly[d(A-T)] was studied with fluorescence temperature-jump methods. Poly[d(A-T)] binds proflavin in a two-step reaction with a preequilibrium. Poly[d(G-C)] is characterized by a bimolecular reaction. The binding of acridines to natural DNAs is shown to be characterized by different types of sites whose properties depend on the base composition. The sites have considerable enthalpic differences which result in exchange of dye molecules between them when the temperature is changed. Also, on natural DNAs A-T base pairs are associated with a rapidly equilibrating external complex which is absent or much weaker for G-C base pairs.
D000166:Acridines; D001410:Bacillus megaterium / Q000032:analysis; D004269:DNA, Bacterial; D007700:Kinetics; D008433:Mathematics; D008837:Micrococcus / Q000032:analysis; D011089:Polydeoxyribonucleotides; D011370:Proflavine; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship
D000166:Acridines; D004269:DNA, Bacterial; D011089:Polydeoxyribonucleotides; D011370:Proflavine