Interaction between lysine-rich histones and DNA.
Using a membrane filter retention technique we have studied the interaction between DNA and lysine rich histone H5 in vitro. It is found that, depending on the ionic conditions, H5 can bind DNA in a random or cooperative manner and exhibits a preference to DNA with high molecular weight and/or high A + T content, as also observed with H1. The presence of 6 M urea in the assay mixture does not impair the selectivity of H5 to A + T rich DNA but partly affects its selectivity to DNA size. In contrast to H1, H5 does not discriminate between the superhelical and relaxed forms of circular SV40 DNA.
D000818:Animals; D002645:Chickens; D004247:DNA; D004912:Erythrocytes; D005374:Filtration / Q000379:methods; D006657:Histones / Q000097:blood; D007700:Kinetics; D008239:Lysine; D008970:Molecular Weight; D011485:Protein Binding; D051381:Rats
D006657:Histones; D004247:DNA; D008239:Lysine