DNA repeat lengths of erythrocyte chromatins differing in content of histones H1 and H5.
Among the erythrocytes of chicken, trout, carp, and sucker, the relative proportion of the lysine-rich histone H5 varied from 20 to 0% of the total histones. Following digestion of nuclear chromatin with micrococcal nuclease, each of them displayed a longer DNA repeat length and greater repeat length heterogeneity than found in liver chromatin. Fish erythrocytes possessed similar repeat lengths of 207-209 base pairs which was 10-12 base pairs shorter than in chicken erythrocyte chromatin and approximately 10 base pairs longer than in liver chromatin. No correlation existed between the DNA repeat length or repeat length heterogeneity and the relative proportion of H5.
D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics; D002645:Chickens / Q000235:genetics; D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000032:analysis / Q000648:ultrastructure*; D005399:Fishes / Q000235:genetics; D006657:Histones / Q000032:analysis* / Q000302:isolation & purification; D008099:Liver / Q000032:analysis; D008239:Lysine; D008836:Micrococcal Nuclease / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D014337:Trout / Q000235:genetics
D002843:Chromatin; D006657:Histones; D004247:DNA; D008836:Micrococcal Nuclease; D008239:Lysine