[Reorganisation of thyroid follicular cells in culture. Effect of collagen on the orientation of cell polarity].
In contact with a collagen gel, thyroid follicular cells form a basal pole. A monolayer, with the apical side facing the culture medium is formed when cells are seeded on a collagen layer. In contrast cells reorganize into follicle-like structures when they are embedded in the collagen gel. If vesicles which have their apical pole in contact with the culture medium are embedded in the gel, collagen-cell interaction induced the inversion of cell polarity giving follicular structures. In addition after being overlaid with a collagen layer, cells forming a monolayer reorgnaize into follicles. The apical pole is formed on the free surface of the monolayer or on the internal side of structures embedded in collagen which differentiate into follicles.
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D003470:Culture Media; D008854:Microscopy, Electron; D013552:Swine; D013961:Thyroid Gland / Q000166:cytology* / Q000648:ultrastructure
D003470:Culture Media; D003094:Collagen
Réorganisation en culture des cellules folliculaires thyroïdiennes. Influence du collagène sur l'orientation de la polarité cellulaire.