Liquid-chromatographic measurement of urinary monosaccharides.
We describe "high-performance" liquid chromatography of urinary monosaccharides on a macroporous anion-exchange resin (Hitachi 3013N), with acetonitrile/water as eluent. The separated xylose, arabinose, D-fructose, mannose, galactose, and D-glucose were detected by means of a post-column reduction reaction of tetrazolium blue. Absolute limits of detection were about 10 ng of monosaccharide. Lactose can also be measured.
D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D005632:Fructose / Q000652:urine; D005690:Galactose / Q000652:urine; D006029:Glycosuria; D006801:Humans; D009005:Monosaccharides / Q000652:urine*; D011247:Pregnancy; D012266:Ribose / Q000652:urine; D014994:Xylose / Q000652:urine
D009005:Monosaccharides; D005632:Fructose; D012266:Ribose; D014994:Xylose; D005690:Galactose