Synthesis of maleimide derivative of cortisol for enzyme coupling is cortisol enzyme immunoassay.
A new cortisol derivative, cortisol-21-m-maleimidobenzoate (CMB), was synthesized and conjugated with sulfhydryl groups of beta-galactosidase (BG). Both CMB-BG and CHS-BG conjugates have a high immunoreactivity to cortisol antibody, and although CHS-BG does not displace well with the added cortisol, CMB-BG does.
D006854:Hydrocortisone / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D007124:Immunoenzyme Techniques; D008301:Maleimides / Q000138:chemical synthesis*; D001616:beta-Galactosidase
D008301:Maleimides; C024367:cortisol-21-3-maleimidobenzoate; D001616:beta-Galactosidase; D006854:Hydrocortisone