Glycosaminoglycans of some mouse mastocytomas.
The glycosaminoglycan fractions of four murine mastocytomas (AB-CBF1-MCT-1, AB-CXBG-MCT-1, AB-CXBI-MCT-1, and Furth), were isolated and characterized using microelectrophoresis, degradation with specific mucopolysaccharidases, paper chromatography and, in some instances, ion-exchange chromatography. Intramuscular and ascites tumors and tumors cultured in vitro were used. The glycosaminoglycans were labeled with [35S]sulfate and , in a few instances, with D-[6-3H]glucosamine. The glycosaminoglycan fraction obtained from the AB-CBF1-MCT-1 mastocytoma cultured in vitro consisted predominantly of dermatan sulfate-like material. On the other hand, the major components of the glycosaminoglycan fractions of the AB-CXBG-MCT-1 and AB-CXBI-MCT-1 and Furth tumors were observed to behave like heparin or heparan sulfate. In most of the mastocytoma samples examined, chondroitin 4-sulfate-like components were found to be present in smaller amounts, even in samples derived from tumor cells cultured in vitro. A component exhibiting a keratan sulfate-like behavior was detected in fractions obtained from the solid CXBG, CXBI and Furth tumors.
D000818:Animals; D002810:Chondroitinases and Chondroitin Lyases / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D004587:Electrophoresis, Agar Gel; D006025:Glycosaminoglycans / Q000032:analysis* / Q000302:isolation & purification; D012515:Mast-Cell Sarcoma / Q000378:metabolism*; D051379:Mice
D006025:Glycosaminoglycans; D002810:Chondroitinases and Chondroitin Lyases