[Techniques for studying collagen in medical practice].
A brief description of the structure of collagen and the special features of each type of collagen, followed by a summary of its metabolism, serve as an introduction to the major pathological processes involving the collagen molecule; mutations, inflammatory syndrome bone diseases, basement membrane diseases. The measurement of hydroxyproline isomers in urine is the basic biochemical test for diseases involving the collagen molecule. 4-hydroxyproline is increased in bone disease of Paget and in cancerous metastases of bone, 3-hydroxyproline is increased in case of polycystic kidney. It is possible, but not very useful, to measure serum 4-hydroxyproline. Of more interest, is the radio-immunological measurement of the N-terminal extension of the type III procollagen molecule, which can differentiate ordinary hepatitis from cirrhogenous hepatitis. New and useful information can be gained from tissue biopsy: the proportion of the various types of collagen, the measurement of their hydroxylation and the activity of the enzymes acting on their biosynthesis.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D001847:Bone Diseases / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D003094:Collagen / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism; D003937:Diagnosis, Differential; D006505:Hepatitis / Q000150:complications / Q000175:diagnosis; D006801:Humans; D006909:Hydroxyproline / Q000652:urine; D007536:Isomerism; D008103:Liver Cirrhosis / Q000150:complications / Q000175:diagnosis; D011347:Procollagen / Q000032:analysis
D000906:Antibodies; D011347:Procollagen; D003094:Collagen; D006909:Hydroxyproline
Méthodes d'étude du collagène en pratique médicale.