Lymphokine production in T-lymphocyte subsets defined by active E-rosette formation.
Human T-cell subsets, defined by active E rosette formation, were examined for their ability to produce leucocyte migration inhibitory factor (LIF) in response to mitogen or specific antigen. It was determined that T cells enriched for active rosette-forming (A+) cells produced LIF in response to concanavalin A, whereas T cells depleted of active rosette-forming (A-) cells did not. Similarly, A+ cells from a tuberculin-sensitive donor produced LIF in response to tuberculin purified protein derivative, whereas A- cells from the same donor failed to produce the mediator. Thus, T-cell production of LIF in humans appears to be restricted to those T cells capable of active rosette formation.
D002469:Cell Separation; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D003208:Concanavalin A