Rapid detection of rinderpest virus antigens by counter-immunoelectrophoresis.
Counter-immunoelectrophoresis was compared with immunodiffusion for its ability to detect rinderpest virus antigens. Counter-immunoelectrophoresis detected antigens in lymph node biopsies from all of 9 infected cattle whereas immunodiffusion detected them in 8 only. Counter-immunoelectrophoresis was between 4 and 16 times more sensitive than immunodiffusion for detecting rinderpest virus antigens in the tissues of diseased animals and could detect positive reactions within 40 min. Counter-immunoelectrophoresis could offer an opportunity for rapid laboratory confirmation of rinderpest.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003378:Counterimmunoelectrophoresis; D005779:Immunodiffusion / Q000662:veterinary; D007122:Immunoelectrophoresis; D008198:Lymph Nodes / Q000276:immunology; D012301:Rinderpest / Q000175:diagnosis*; D012302:Rinderpest virus / Q000276:immunology*; D013154:Spleen / Q000276:immunology
D000956:Antigens, Viral