Preparation of a defined immune complex for use in C1q binding assays.
Soluble immune complexes of human beta 2 microglobulin with its IgG antibodies from Macaca irus antisera have been tested for suitability as a reference preparation for immune complex assays in human disease. The defined complex described is stable on storage, behaves reproducibly in a C1q solid phase binding assay and fulfills the criteria required for an international reference standard.
D000818:Animals; D000936:Antigen-Antibody Complex; D001665:Binding Sites; D003172:Complement C1; D005612:Freeze Drying; D000882:Haplorhini; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G; D011309:Preservation, Biological; D011817:Rabbits
D000936:Antigen-Antibody Complex; D003172:Complement C1; D007106:Immune Sera; D007074:Immunoglobulin G