Sodium n-butyrate induces prostaglandin synthetase activity in mastocytoma P-815 cells.
Cultured mouse mastocytoma P-815 cells were treated with 1 mM sodium n-butyrate for 40 h. The treated cell homogenate showed high activities in synthesizing prostaglandin D2, E2, and F2 alpha. Such activities were virtually absent in untreated cell homogenate. Direct addition of sodium n-butyrate to the homogenate showed no effects. Pre-exposure of cells to acetylsalicylic acid did not diminish the effect of the subsequent treatment with sodium n-butyrate. These data suggest that sodium n-butyrate induces fatty acid cyclooxygenase in P-815 cells.
D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D002087:Butyrates / Q000494:pharmacology*; D002478:Cells, Cultured; D004790:Enzyme Induction; D012515:Mast-Cell Sarcoma / Q000201:enzymology*; D051379:Mice; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis*
D002087:Butyrates; D011451:Prostaglandin-Endoperoxide Synthases; D001241:Aspirin