[Kinetic method of registering cell agglutination in suspension].
A method based on changes in cell light scattering during agglutination is proposed for registration of cell agglutination in suspension. Unlike other methods of investigation of cell agglutination, our measurements are made under constant stirring and minimal stress tension in specially constructed cells. Possibilities of the method are shown upon registration of agglutination of trypsinized rabbit erythrocytes caused by PHA, Ca ions or by a sample containing lectin isolated from the fresh water algae Chara. The sensitivity of our method is higher, than that with the microtitration according to Tacashi, and makes it possible to investigate the agglutination process kinetics.
D000371:Agglutination / Q000187:drug effects; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003584:Cytological Techniques; D004903:Erythrocyte Aggregation / Q000187:drug effects; D007700:Kinetics; D037102:Lectins / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D011817:Rabbits; D013535:Suspensions
D037102:Lectins; D010835:Phytohemagglutinins; D013535:Suspensions; D002118:Calcium
Kineticheskiĭ metod registratsii aggliutinatsii keltok v suspenzii.