A novel immunization procedure involving pretreatment with cross-reacting steroid conjugated to a copolymer of D-glutamic acid and D-lysine for production of low cross-reactive antisera.
Anti-testosterone and anti-5 alpha-dihydrotestosterone antisera have been produced by pretreatment of mice with a cross-reacting steroid coupled to a copolymer of D-glutamic acid and D-lysine to inhibit production of cross-reacting antibodies before immunization with the specific steroid conjugated to a protein. 15 beta-Carboxyethylmercapto derivatives were used as haptens. The cross-reactivities of the anti-testosterone and anti-5 alpha-dihydrotestosterone antisera with 5 alpha-dihydrotestosterone and with testosterone were 0.49 and 13.5%, respectively.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003429:Cross Reactions; D013196:Dihydrotestosterone / Q000276:immunology*; D005971:Glutamates; D007106:Immune Sera; D008239:Lysine; D051379:Mice; D011108:Polymers; D013739:Testosterone / Q000276:immunology*
D005971:Glutamates; D007106:Immune Sera; D011108:Polymers; D013196:Dihydrotestosterone; D013739:Testosterone; D008239:Lysine