[De novo lipogenesis: kinetics of in vivo incorporation of tritiated water 3H into fatty acids and total lipids of the liver, plasma, adipose tissue and carcass of the male rat].
Tritiated water 3H, injected by intraperitoneal route into 7-week old male Rats, was incorporated into lipids synthesized de novo. The Rats were killed 0, 3, 7, 10, 15, 30, 60 and 120 min. after tracer injection. The results show that an optimal interval of about 10 min. between tracer injection and animal sacrifice was necessary to obtain a correct estimate of lipogenesis de novo by avoiding intertissue exchanges.
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D005227:Fatty Acids / Q000096:biosynthesis / Q000097:blood; D007700:Kinetics; D008055:Lipids / Q000096:biosynthesis* / Q000097:blood; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D014316:Tritium; D014867:Water / Q000378:metabolism
D005227:Fatty Acids; D008055:Lipids; D014867:Water; D014316:Tritium
Etude de la lipogenèse de novo: cinétique d'incorporation in vivo du 3H de l'eau tritiée dans les acides gras et les lipides totaux du foie, du plasma, du tissu adipeux et la carcasse du Rat mâle.