Euglena gracilis chloroplast EF-Ts. Evidence that it is a nuclear-coded gene product.
Extracts of Euglena gracilis cells contain high levels of elongation factor (EF)-Ts (EF-Tschl) activity which can be assayed by measuring the rate of exchange of GDP with Escherichia coli EF-Tu . GDP. The appearance of EF-Ts activity in E. gracilis cells is light-stimulated, suggesting that the EF-Ts is required for chloroplast function. However, based on experiments with a mutant of E. gracilis lacking chloroplast DNA, as well as studies on the effect of antibiotics on EF-Ts synthesis, it is concluded that the EF-Tschl gene is nuclear-coded.
D002467:Cell Nucleus / Q000378:metabolism; D002474:Cell-Free System; D002736:Chloroplasts / Q000378:metabolism*; D003624:Darkness; D005056:Euglena gracilis / Q000235:genetics / Q000378:metabolism*; D005796:Genes; D006153:Guanosine Diphosphate / Q000378:metabolism; D008027:Light; D010445:Peptide Elongation Factors / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
D010445:Peptide Elongation Factors; D006153:Guanosine Diphosphate