A specific enzyme-linked immunosorbent assay for determination of bovine IgM: use in screening hybridoma cell lines.
A highly specific enzyme-linked immunosorbent assay (ELISA) is described for determination of bovine IgM and use in screening IgM-secreting hybridoma cell lines. The advantages of this assay over the method of radioactive labelling of cells followed by immune precipitation, SDS-PAGE, fluorography and autofluorography are demonstrated.
D000818:Animals; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006824:Hybridization, Genetic; D006825:Hybridomas / Q000032:analysis* / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000032:analysis* / Q000378:metabolism
D007075:Immunoglobulin M