A new general approach to fluorogenic enzyme assays of high sensitivity and potential high specificity, illustrated by a procedure for beta-D-galactosidase estimations.
D002845:Chromatography; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000201:enzymology; D005456:Fluorescent Dyes; D005696:Galactosidases / Q000378:metabolism*; D006027:Glycosides / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D006923:Hymecromone / Q000031:analogs & derivatives / Q000096:biosynthesis*; D008163:Luminescent Measurements; D013050:Spectrometry, Fluorescence; D014468:Umbelliferones / Q000096:biosynthesis*; D001616:beta-Galactosidase / Q000378:metabolism*
D005456:Fluorescent Dyes; D006027:Glycosides; D014468:Umbelliferones; D006923:Hymecromone; C024791:methylumbelliferyl 2-acetamido-2-deoxy-beta-D-lactoside; D005696:Galactosidases; D001616:beta-Galactosidase