Nucleotide sequence of non-initiator methionine tRNA from Bacillus subtilis.
Non-initiator methionine tRNA (tRNAMet) was purified from Bacillus subtilis W 168 by a consecutive use of several column chromatographic systems. The nucleotide sequence was determined to be p-G-G-C-G-G-U-G-U-A-G-C-U-C-A-G-C-G-G-C-D-A-G-A-G-C-G-U-A-C-G-G-U-U-C-A-U-m6A-C-C-C -G-U-G-A-G-G(m7G)-U(D)-C-G-G-G-G-G-T-psi-C-G-A-U-C-C-C-C-U-C-C-G-C-C-G-C-U-A-C- C-A-OH. The nucleosides of G46 and U47 were partially modified to m7G and D, respectively. The nucleotide sequence shows a unique feature that the position adjacent to 3'-end of the anticodon C-A-U is occupied by m6A, not by t6A, although the tRNAMet belongs to a groups of tRNAs which recognize codons starting with A.
D000926:Anticodon; D001412:Bacillus subtilis / Q000032:analysis*; D001483:Base Sequence; D003062:Codon; D008715:Methionine; D009690:Nucleic Acid Conformation; D012343:RNA, Transfer
D000926:Anticodon; D003062:Codon; D012343:RNA, Transfer; D008715:Methionine