Immunodepletion of human plasma factor VIII.
Affinity chromatography of human cryosupernatants on anti-human factor VIII-Sepharose yielded a plasma devoid of detectable factor VIIIC, VIIIR:Ag, and VIIIR:WF activities. This plasma was indistinguishable from severe congenital hemophilic plasma when used as substrate in factor VIII coagulant assays.
D000042:Absorption; D000818:Animals; D000906:Antibodies / Q000502:physiology; D001780:Blood Coagulation Tests; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D005169:Factor VIII / Q000302:isolation & purification*; D006467:Hemophilia A / Q000097:blood; D006801:Humans; D007163:Immunosorbent Techniques; D011817:Rabbits; D014841:von Willebrand Factor / Q000032:analysis
D000906:Antibodies; D014841:von Willebrand Factor; D005169:Factor VIII