The relation between toxicity and toxin-related-antigen contents of Clostridium botulinum types C and D cultures as determined by mouse bioassay and ELISA.
ELISA was tested for the adequacy to differentiate between botulinum type C1 and D toxins. The results presented in this paper indicate antigenic relationship between type C1 and D toxins. Furthermore, indications were obtained that the antigenicities of type C1 and of D toxins produced by different strains are not identical. Although ELISA can be used to differentiate type C1 and D toxins from those of other types, the assay has only limited value to differentiate between type C1 and D toxins. No parallel relationship between toxicity (mouse bioassay) and toxin-related-antigen contents (ELISA) of C. botulinum type C and D cultures was found.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D001681:Biological Assay; D001905:Botulinum Toxins / Q000032:analysis* / Q000276:immunology / Q000633:toxicity; D003014:Clostridium botulinum / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D009500:Neutralization Tests
D000942:Antigens, Bacterial; D001905:Botulinum Toxins