[Continuous detection of the release of acetylcholine from the electric organ of Torpedo using a chemiluminescence reaction].
A chemiluminescent procedure to measure acetylcholine was developed and used to detect continuously the release of transmitter from stimulated electric organ slices and synaptosomes. Acetylcholine is hydrolysed by acetylcholinesterase; the choline liberated is oxidized by choline oxidase to betaïne; the H2O2 generated triggers the luminescence of luminol in presence of peroxidase. The extension to other transmitters is possible; several developments are envisaged.
D000109:Acetylcholine / Q000032:analysis*; D000110:Acetylcholinesterase; D000429:Alcohol Oxidoreductases; D000818:Animals; D004557:Electric Organ / Q000378:metabolism*; D005399:Fishes; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol; D010544:Peroxidases
D008165:Luminol; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D010544:Peroxidases; D000110:Acetylcholinesterase; D000109:Acetylcholine
Détection continue de la libération d'acétylcholine de l'organe électrique de la Torpille à l'aide l'une réaction de chimiluminescence.