[Preparation of monospecific anti-Salmonella LPS antibodies from bacteria immobilized in polyacrylamide gel].
The use of an immunoabsorbent obtained by entrapping Salmonella cells into a polyacrylamide gel lattice enabled us to obtain anti-O monospecific immune sera which can be used for a quick serological identification of some species of Salmonella in the course of a diagnosis. In this paper we describe a method for immobilization of S. typhimurium as well as the preparation of monospecific anti-05 antibodies from plurispecific anti-S. haifa rabbit immune sera. This separation of anti-O monospecific antibodies avoids the repeated and often tedious adsorption of anti-Salmonella immune sera by the corresponding whole bacteria. Such Salmonella, entrapped into the polyacrylamide gel lattice, can be used several times without appreciable loss of their affinity properties.
D000180:Acrylic Resins / Q000494:pharmacology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D007158:Immunologic Techniques; D007164:Immunosorbents / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000276:immunology*; D011817:Rabbits; D012480:Salmonella Infections / Q000175:diagnosis* / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology*
D000180:Acrylic Resins; D000907:Antibodies, Bacterial; D007164:Immunosorbents; D008070:Lipopolysaccharides; C016680:polyacrylamide gels
Préparation d'anticorps monospécifiques anti-LPS de Salmonella sur bactéries immobilisées dans un gel de polyacrylamide.