Role of T lymphocytes in the response to TNP-AECM-Ficoll.
The T cell dependence of the in vivo and in vitro immune response to TNP-Ficoll was re-examined. It was found that after extensive T cell depletion, the in vitro response to TNP-Ficoll was significantly reduced and could be restored with T cells. Despite the apparent T cell dependence, TNP-Ficoll was able to elicit responses by congenitally athymic BALB/c mice in vivo and by spleen cells from these mice in vitro. The in vitro response of normal spleen cells to TNP-Brucella abortus was occasionally diminished as a result of T cell depletion but never as drastically as the response to TNP-Ficoll. This difference in requirement for T cell influence correlates well with our prior classification of these antigens into TI-1 and TI-2 categories.
D000818:Animals; D000942:Antigens, Bacterial; D002003:Brucella abortus / Q000276:immunology; D005260:Female; D005362:Ficoll / Q000031:analogs & derivatives / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D007075:Immunoglobulin M / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008819:Mice, Nude; D009578:Nitrobenzenes / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology*
D000942:Antigens, Bacterial; D007075:Immunoglobulin M; D009578:Nitrobenzenes; D011134:Polysaccharides; C026595:TNP-AECM-ficoll; D014301:Trinitrobenzenes; D005362:Ficoll