A new candidate for the regulation of erythropoiesis. Insulin-like growth factor I.
The effect of pure human insulin-like growth factor I (IGF I) on the colony formation of late stage erythroid precursor cells (CFU-e) from fetal mouse liver and adult bone marrow was studied in a serum-free culture system. We found that IGF I in physiological concentrations stimulated erythroid colony formation. The combined effect of IGF I and erythropoietin was smaller than the sum of their single effects. The number of colonies induced by IGF I was linearly dependent on the number of plated cells. Our results indicate that IGF I is the first clearly defined mitogen that stimulates the late stages of erythroid differentiation independently of erythropoietin.
D000818:Animals; D001681:Biological Assay; D001853:Bone Marrow / Q000502:physiology; D004920:Erythropoiesis / Q000187:drug effects*; D004921:Erythropoietin / Q000302:isolation & purification / Q000494:pharmacology; D005260:Female; D005333:Fetus; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy; D013002:Somatomedins / Q000494:pharmacology*
D007328:Insulin; D013002:Somatomedins; D004921:Erythropoietin