[Effect of polB mutation on the nature of the thymineless death of thy- cells of Escherichia coli K-12].
Cell survival, the rates of DNA and protein synthesis, stabilization and repair of single-stranded DNA breaks were measured for Escherichia coli K-12 pol+, polA1, polB1, polC(ts) cells during thymine deprivation. The results indicate that single-stranded breaks in DNA induced by thymine deprivation play no major role in thymineless death of thy- polB- cells, which is similar to thy recB mutant lacking exonuclease V. Probably, the dependence of exonuclease V activity on the presence of DNA polymerase II (the polB gene product) exists which determines molecular mechanisms of thymineless death of thy- polB- cells.
D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D013941:Thymine / Q000172:deficiency* / Q000378:metabolism
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004259:DNA-Directed DNA Polymerase; D013941:Thymine
Vliianie polB-mutatsii na kharakter bestiminovoĭ gibeli thy- -kletok Escherichia coli K-12.