Purification of human C5a des arg by immunoadsorbent and molecular sieve chromatography.
Human C5a des arg was isolated from complement-activated serum by immunoadsorption followed by Sephadex G-75 chromatography. C5a des arg obtained by this 2-step procedure was shown to be immunologically identical to C5a des arg purified by a conventional multi-step method, homogeneous on SDS-polyacrylamide gels, and biologically active. Although this technique yields approximately the same amount of C5a des arg/liter of activated serum as that obtained by conventional methods, its simplicity and relative rapidity make it a practical alternative.
D000818:Animals; D002634:Chemotaxis, Leukocyte; D002850:Chromatography, Gel; D003167:Complement Activation; D003182:Complement C5 / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D015937:Complement C5a, des-Arginine; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats; D006801:Humans; D007163:Immunosorbent Techniques; D008247:Lysosomes / Q000378:metabolism; D009504:Neutrophils
D003182:Complement C5; D015937:Complement C5a, des-Arginine