[Investigation of in vitro cultivated cells from smooth muscular organs. 5. Immunohistochemical experiments for demonstration of myosin and estimation of changes in the myosin content in the aorta and in isolated respectively cultivated aortic cells of newborn rats (author's transl)].
Smooth muscle myosin was demonstrated in cryostat sections, isolated and cell cultures from aortas of newborn rats by the method of indirect immunofluorescence. The content of myosin in the cells was compared by measuring the fluorescence intensity of single cells. Well differentiated smooth muscle cells present in suspensions of the whole aorta and of the aortic media as well as in aortic cell cultures, are shown to have a higher myosin content than fibroblasts of adventitia and smooth muscle cells with a lower degree of differentiation.
D000818:Animals; D000831:Animals, Newborn; D001011:Aorta; D002478:Cells, Cultured; D005347:Fibroblasts / Q000032:analysis; D005455:Fluorescent Antibody Technique; D009131:Muscle, Smooth, Vascular / Q000032:analysis* / Q000166:cytology; D009218:Myosins / Q000032:analysis*; D051381:Rats
D009218:Myosins
Untersuchungen in vitro kultivierter Zellen glattmuskulärer Organe 5. Mitteilung: Immunhistochemische Untersuchungen zum Nachweis von Myosin und zur Erfassung von Anderungen des Myosingehaltes in der Aorta sowie in isolierten oder kultivierten Aortenzellen neonataler Ratten.