Genetic tests for the detection of chemically induced small deletions in Drosophila chromosomes.
We have developed genetic tests for estimating the proportion of small deletions among chemically induced point mutations in Drosophila. The criteria used allow the detection of deletions that are large enough to include a viable visible mutation as well as a lethal, or a sex-linked lethal as well as a gene that is required for the development of a spermatogonium into a spermatozoon. On these criteria, we have concluded that DEB produces a high proportion of deletions among point mutations; that HA produces no deletions; and that DEN produces either no deletions or only very small ones that cannot be detected by our methods.
D000818:Animals; D002073:Butanes / Q000494:pharmacology*; D002872:Chromosome Deletion; D004052:Diethylnitrosamine / Q000494:pharmacology*; D004331:Drosophila melanogaster / Q000235:genetics; D004852:Epoxy Compounds / Q000494:pharmacology*; D004988:Ethers, Cyclic / Q000494:pharmacology*; D005020:Ethyl Methanesulfonate / Q000494:pharmacology*; D005260:Female; D005821:Genetic Techniques; D006898:Hydroxylamines / Q000494:pharmacology*; D008297:Male; D009030:Mosaicism; D009154:Mutation; D009602:Nitrosamines / Q000494:pharmacology*; D013093:Spermatogonia / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects
D002073:Butanes; D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D006898:Hydroxylamines; D009602:Nitrosamines; D004052:Diethylnitrosamine; C007366:diepoxybutane; D005020:Ethyl Methanesulfonate