Acidic thiol proteinase activity of Schistosoma mansoni cultured in vitro.
Schistosoma mansoni cultured in vitro for periods from 12 days to 33 weeks were analyzed for proteinase activity using a fluorescent substrate and compared with adult S. mansoni worms from infected mice. The enzyme activity measured was that of an acidic thiol-dependent proteinase previously described. Proteinase activity appears early in development, and rises rapidly to an approximately constant proportion of the total extractable worm protein. Cultured worms have 5 to 20 times higher specific activity than in vivo-developed worms. However, cultured worms and in vivo worms have approximately equivalent activities per worm.
D000818:Animals; D003546:Cysteine Endopeptidases; D010450:Endopeptidases / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D012550:Schistosoma mansoni / Q000201:enzymology* / Q000254:growth & development; D012552:Schistosomiasis / Q000469:parasitology
D010450:Endopeptidases; D003546:Cysteine Endopeptidases