[Isolation, depolymerization and repolymerization of flagella of Pseudomonas aeruginosa (author's transl)].
Flagellar filaments of P. aeruginosa are isolated and purified by cultivating the cells in fluid medium, followed by shaking in a rotation-homogenizer and by fractional centrifugation. The electronmicroscopically pure preparations are partially disintegrated by heating at 60-100 degrees C/30 min. The flagellin repolymerizes spontaneously into flagella-like but straight filaments. In the indirect fluorescent antibody test, isolated flagellar filaments and polymerized flagellin-filaments with antiflagellar antibodies like flagella of native cells.
D003470:Culture Media; D005407:Flagella / Q000648:ultrastructure*; D005408:Flagellin / Q000378:metabolism; D006358:Hot Temperature; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000648:ultrastructure*; D014465:Ultrasonics
D003470:Culture Media; D005408:Flagellin
Isolierung, Depolymerisierung und Repolymerisierung der Geisseln von Pseudomonas aeruginosa.