Immunological characterization of catfish (Ictalurus punctatus) insulin and proinsulin.
1. Insulin and insulin intermediates were isolated and characterized from catfish (Ictalurus punctatus) pancreatic islets. 2. An antibody developed to this catfish insulin allowed the identification of proinsulin, and insulin intermediates. 3. Amino acid composition of the isolated insulin intermediates was similar to the calculated amino acid composition of proinsulin, as determined from the analyses of the separate A, B and C chains.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000917:Antibody Formation; D002096:C-Peptide / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes / Q000276:immunology*; D007328:Insulin / Q000276:immunology*; D007700:Kinetics; D010084:Oxidation-Reduction; D011384:Proinsulin / Q000276:immunology*
D000596:Amino Acids; D002096:C-Peptide; D007328:Insulin; D011384:Proinsulin