Detection of primase specified by IncB plasmid R864a.
Plasmid R864a (IncB) contains nucleotide sequences homologous with the sog primase determinant of IncI alpha plasmid ColIb-P9. Extracts of Escherichia coli carrying a mutant R864a derepressed for transfer functions showed enhanced primase activity, and contained a large polypeptide identical in size (apparent Mr = 220,000) to the IncI alpha sog gene product.
D000483:Alleles; D019915:DNA Primase; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D010957:Plasmids; D012316:RNA Nucleotidyltransferases / Q000235:genetics* / Q000302:isolation & purification
D004274:DNA, Recombinant; D046911:Macromolecular Substances; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases