Preparation of water-soluble high-molecular weight substrates for beta-D-galactosidase.
Two types of high-molecular water-soluble substrates of beta-D-galactosidase were prepared. Substrate I contains beta-D-[3H]-galactopyranosyl moieties linked, through a hydrocarbon bridge, to a polymeric dialdehyde (oxidized starch) backbone (molecular weight 6,000); in substrate II the backbone is poly-L-lysine (molecular weight 80,000). In the presence of Triton X-100, but not in its absence, D-[3H]-galactose is split from the substrates by homogenates of normal mouse liver or pancreas. It is suggested that substrates I and II could be used to test the integrity of lysosomal and other cellular membranes, and to assess the extent of cellular injury.
D000447:Aldehydes / Q000138:chemical synthesis / Q000378:metabolism; D000818:Animals; D004926:Escherichia coli / Q000201:enzymology; D005690:Galactose / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000378:metabolism; D005696:Galactosidases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology; D051379:Mice; D008970:Molecular Weight; D017830:Octoxynol; D010179:Pancreas / Q000201:enzymology; D011092:Polyethylene Glycols / Q000494:pharmacology; D011107:Polylysine / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000378:metabolism; D011108:Polymers / Q000138:chemical synthesis / Q000378:metabolism*; D012995:Solubility; D013379:Substrate Specificity; D001616:beta-Galactosidase / Q000378:metabolism*
D000447:Aldehydes; D011108:Polymers; D011107:Polylysine; D011092:Polyethylene Glycols; D017830:Octoxynol; D005696:Galactosidases; D001616:beta-Galactosidase; D005690:Galactose