Human blood platelet membrane glycoproteins. Resolution in different polyacrylamide gel electrophoretic systems.
The separation of the major platelet membrane glycoproteins of normal subjects and subjects with well defined platelet membrane glycoprotein abnormalities have been examined using four different polyacrylamide gel electrophoretic techniques (continuous and discontinuous). The mobilities of the resolved glycoprotein bands have been correlated with the glycoprotein nomenclature proposed for the conventional sodium dodecyl sulphate-phosphate buffer system. Since the glycoprotein distribution varies depending on the system used, the merits of each method should be considered before application to a specific problem.
D000085:Acetates; D000178:Acrylamides; D001792:Blood Platelets / Q000032:analysis*; D002021:Buffers; D002462:Cell Membrane / Q000032:analysis; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D005998:Glycine; D006023:Glycoproteins / Q000302:isolation & purification*; D006801:Humans; D008565:Membrane Proteins / Q000302:isolation & purification*; D008970:Molecular Weight; D010710:Phosphates; D014325:Tromethamine; D014508:Urea
D000085:Acetates; D000178:Acrylamides; D002021:Buffers; D006023:Glycoproteins; D008565:Membrane Proteins; D010710:Phosphates; D014325:Tromethamine; D014508:Urea; D004492:Edetic Acid; D005998:Glycine