[GABA fluophore formation due to ninhydrin reaction in the octanolic milieu. Spectrofluorometric investigation. 1. (author's transl)].
As a result of the reaction of ninhydrin with gamma-aminobutyric acid and glutamic acid in the octanolic milieu, a fluorescent product, presumably a copper-II-chelat-complex, was formed. This appears analogous to the fluorescence histochemical procedure. the reaction product displays the following spectrofluorometric properties: excitation peak at lambda = 375 nm and emission plaximum at lambda = 445 nm. Spectrofluorometric estimations during performing various steps of the reaction were indicative of possibilities of the improvement of the fluorescence histochemical GABA demonstration.
D005971:Glutamates; D009555:Ninhydrin; D000442:Octanols; D012997:Solvents; D013050:Spectrometry, Fluorescence; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D005971:Glutamates; D000442:Octanols; D012997:Solvents; D005680:gamma-Aminobutyric Acid; D009555:Ninhydrin
gamma-Aminobuttersüre- (GABA-) Fluorescenz bei der Ninhydrin-Reaktion im octanolischen Milieu. Spektrofluorometrische Untersuchung 1. Mitteilung.