Cellular changes in bone marrow of malaria-infected mice. III. Chemotaxis of granulocytes.
The number of bone marrow cells and their chemotactic activity was studied during malaria infection. Two days after infection of Balb/c mice with Plasmodium berghei, an increase in granulocyte number was observed in the blood. A modified Boyden chamber chemotaxis assay was employed to investigate the mechanism of granulocyte accumulation in the blood. Bone marrow cells from normal mice, from mice during a primary lethal infection and from immune mice after challenge were compared. The complement factor C5a showed chemotactic activity for bone marrow cells; a significant decrease of chemotaxis was only observed after 6 days of primary infection. Extracts of spleen, liver and infected erythrocytes lacked chemotactic activity, or caused inhibition of cell migration. Serum from mice with a 2-day primary infection contained chemotactic activity. The active component was heat labile, protease sensitive and had an estimated molecular weight of 250,000.
D000818:Animals; D001790:Blood Physiological Phenomena; D001853:Bone Marrow / Q000473:pathology*; D002634:Chemotaxis, Leukocyte; D003182:Complement C5 / Q000494:pharmacology; D015936:Complement C5a; D006098:Granulocytes / Q000502:physiology; D007958:Leukocyte Count; D008110:Liver Extracts / Q000494:pharmacology; D008288:Malaria / Q000097:blood* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010962:Plasmodium berghei; D013154:Spleen; D014020:Tissue Extracts / Q000494:pharmacology
D003182:Complement C5; D008110:Liver Extracts; D014020:Tissue Extracts; D015936:Complement C5a