Improvement of the demonstration of A, B, H surface antigens in bladder tumors by a simple immunofluorescence technique.
The presence of A, B and H blood group antigens in paraffin-embedded tissue sections was demonstrated with a routine direct and indirect immunofluorescence technique. Natural and human antisera were used for 102 biopsy specimens of the various blood groups, including normal mucosa and vesical tumors. This technique allows a more precise description than the specific red cell adherence reaction of the differentiation pattern of the tumor itself and of the surrounding and distant mucosa. It may prove useful in suggesting a revised classification system, with more diagnostic and prognostic significance.
D000017:ABO Blood-Group System / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007104:Immune Adherence Reaction; D007106:Immune Sera; D037102:Lectins; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
D000017:ABO Blood-Group System; D000954:Antigens, Surface; D007106:Immune Sera; D037102:Lectins