Characterization of human lysozyme (muramidase)-releasing cells. Organ distribution and functional properties as analyzed in a protein A plaque assay.
Using a modification of the protein A plaque assay, human lysozyme-releasing cells were detected as plaque-forming cells (PFC). Blood and bone marrow contained higher numbers of muramidase secretors compared to adenoid and tonsil cell suspensions. Human peripheral blood cell cultures were found to contain cells detected as PFC in the presence of antimuramidase immunoglobulin as a developing agent. Lysozyme-producing cells were also found in human bone marrow cultures. High cell densities and a short culture period were optimal conditions for lysozyme release in bone marrow cell cultures. Addition of the activating ligand lipopolysaccharide directly into the plaque assay altered the number of muramidase-secreting cells.
D000921:Antibody-Producing Cells / Q000502:physiology*; D006462:Hemolytic Plaque Technique; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008221:Lymphoid Tissue / Q000166:cytology; D008722:Methods; D009113:Muramidase / Q000378:metabolism*; D013205:Staphylococcal Protein A
D008070:Lipopolysaccharides; D013205:Staphylococcal Protein A; D009113:Muramidase