A Dictyostelium discoideum DNA fragment complements a Saccharomyces cerevisiae ura3 mutant.
Dictyostelium discoideum DNA fragments have been inserted into the chimeric bacterium-yeast plasmid YEp13. Recombinant plasmids were used to transform yeast using a strain of Saccharomyces cerevisiae deficient in OMP decarboxylase activity. Several clones were selected for growth in uracil-free medium. One clone was further analysed and contains a plasmid with a segment of D. discoideum DNA which complements a yeast ura3 mutation.
D004271:DNA, Fungal / Q000235:genetics; D004274:DNA, Recombinant; D004278:DNA, Superhelical / Q000235:genetics; D004023:Dictyostelium / Q000235:genetics* / Q000254:growth & development; D005786:Gene Expression Regulation; D005816:Genetic Complementation Test; D008938:Mitosis; D009154:Mutation; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014498:Uracil / Q000378:metabolism*
D004271:DNA, Fungal; D004274:DNA, Recombinant; D004278:DNA, Superhelical; D014498:Uracil