Isolation and structure of several peptides from porcine hypothalami.
Ten peptides were isolated from porcine hypothalami and structurally elucidated. These included four dipeptides Arg-Phe, Phe-Tyr, Val-Trp, and Tyr-Phe; a tripeptide Lys-Phe-Tyr; two tetrapeptides Gly-Lys-Val-Asn and Phe-Glu-His-Glu, a nonapeptide Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe; a decapeptide Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe and a hexadecapeptide Phe-Leu-Gly-Phe-Pro-Thr-Thr-Lys-Thr-Tyr-Phe-Pro-His-Phe-Asn-Leu. The tetrapeptide Gly-Lys-Val-Asn, the nonapeptide, the decapeptide and the hexadecapeptide most probably represent artifact fragments of alpha- and beta-chains of porcine hemoglobin. The natural or synthetic Gly-Lys-Val-Asn and Phe-Glu-His-Glu had some growth hormone releasing activity while Val-Trp, Tyr-Phe and Lys-Phe-Tyr had slight prolactin releasing activity. The biological activities of other peptides have not been determined yet.
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D013007:Growth Hormone-Releasing Hormone; D007028:Hypothalamic Hormones / Q000302:isolation & purification*; D007031:Hypothalamus / Q000032:analysis*; D009842:Oligopeptides / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D010902:Pituitary Gland / Q000187:drug effects; D051381:Rats; D013552:Swine; D013973:Thyrotropin-Releasing Hormone
D007028:Hypothalamic Hormones; D009842:Oligopeptides; D010455:Peptides; D013973:Thyrotropin-Releasing Hormone; D013007:Growth Hormone-Releasing Hormone