[The quantitative immunochemical determination of specific antibodies against Candida albicans within the scope of immunological testing in the malignant melanoma (author's transl)].
Precipitating antibodies against a polysaccharide antigen from Candida albicans were in the human serum determined quantitatively by the aid of a reversed radial immunodiffusion technique. The gel, therewith, contained the antigens, whilst into the gel's punching holes the serum specimens and the calibrated standards were filled in. Amplifying examinations of the course in patients suffering from malignant melanoma, the findings of the methods used hitherto were compared within the scope of an immunological status (in vivo-skin testing by dinitrochlorobenzene, Candida antigen, trichophytin, and tuberculin, precipitation test after Ouchterlony, immunofluorescence-optical evidence of Candida antibodies as well as determination of the Candida antibodies. In contrast to the hitherto available qualitative or semi-quantitative methods evidencing humoral antibodies, we consider the feasibility of simply workable quantitative determinations of antibodies to be an enrichment of the scale of methods. The reversed Mancini technique, simultaneously, is appropriate to determine specific antibodies against other diagnostically relevant antigens, so far as precipitating antigen/antibody complexes are formed. Therewith, commercially available testing kits could favour the standardization.
D000908:Antibodies, Fungal / Q000032:analysis*; D002176:Candida albicans / Q000276:immunology*; D002177:Candidiasis / Q000175:diagnosis; D006801:Humans; D005779:Immunodiffusion; D008545:Melanoma / Q000150:complications / Q000276:immunology*
D000908:Antibodies, Fungal
Die quantitative immunchemische Bestimmung spezifischer Antikörper gegen Candida albicans im Rahmen eines Immunstatus beim malignen Melanom.