Isolation of a stable cell wall-defective form of Neisseria gonorrhoeae from a case of untreated gonococcal urethritis.
A cell wall-defective form of Neisseria gonorrhoeae from an exudate of an untreated patient with gonococcal urethritis was isolated on medium containing 7% polyvinylpyrrolidone. Initial attempts to grow the organism by standard microbiological methods had failed. This isolate was incapable of reversion to a normal gonococcus even after numerous subcultures and appeared to be a stable cell wall-defective form.
D002473:Cell Wall; D003470:Culture Media; D006069:Gonorrhea / Q000382:microbiology*; D006801:Humans; D007740:L Forms / Q000254:growth & development; D009344:Neisseria gonorrhoeae / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D011205:Povidone; D014526:Urethritis / Q000382:microbiology*
D003470:Culture Media; D011205:Povidone