Studies on acid protease fractions and gastric mucosal atrophy. 1. A modified simple method for separate determination of alkali labile pepsin in gastric juice by alkali treatment or by using a synthetic substrate.
A modified simple method applicable for clinical purpose for separate determination of "pepsin" and "gastricsin" by alkali treatment is reported. The method includes dialysis against 0.1 M acetate buffer, pH 5.3 and incubation in an equal volume of 0.1 M phosphate buffer, pH 7.8 to give a final pH of 7.25, at 25 degrees C for 20 minutes, and has a good reproducibility. N-acetyl-L-phenylalanyl-L-diiodotyrosine (APDT) hyprolysing activity and alkali labile pepsin activity are correlated very well. Nevertheless the former cannot completely be destroyed by the treatment.
D000468:Alkalies; D001284:Atrophy; D004151:Dipeptides; D005750:Gastric Juice / Q000032:analysis*; D005753:Gastric Mucosa / Q000201:enzymology* / Q000473:pathology; D006454:Hemoglobins; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010434:Pepsin A / Q000032:analysis*; D010437:Peptic Ulcer / Q000201:enzymology; D010447:Peptide Hydrolases / Q000032:analysis*
D000468:Alkalies; D004151:Dipeptides; D006454:Hemoglobins; C004980:N-acetylphenylalanyl-3,5-diiodotyrosine; D010447:Peptide Hydrolases; D010434:Pepsin A; C020557:gastricsin