Fluorescent method for testing the enzymic activity of mycobacteria.
The possibility of utilizing derivatives of 4-methylumbelliferone (4-MUBF) for the determination of activity of enzymes from the group of hydrolyses was tested in 20 strains of 12 species of mycobacteria. The method proved to be suitable especially for determination of arylsulphatase, beta-D-galactose and acid phosphatase.
D000135:Acid Phosphatase / Q000378:metabolism*; D001192:Arylsulfatases / Q000378:metabolism*; D005696:Galactosidases / Q000378:metabolism*; D006923:Hymecromone / Q000031:analogs & derivatives / Q000378:metabolism; D009161:Mycobacterium / Q000201:enzymology*; D013045:Species Specificity; D013050:Spectrometry, Fluorescence; D013429:Sulfatases / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism*
D006923:Hymecromone; D000135:Acid Phosphatase; D013429:Sulfatases; D001192:Arylsulfatases; D005696:Galactosidases; D001616:beta-Galactosidase