Separation of amino sugars and related compounds by two-dimensional thin-layer chromatography.
Separation of amino compounds, including galactosamine and glucosamine, found in lipopolysaccharide and peptidoglycan, can be accomplished by two-dimensional thin-layer chromatography on cellulose layers. The solvent systems used are: 2-propanol-90% formic acid-water (80:4:20,v/v) in the first dimension and lutidine-water (65:35, v/v) in the second dimension. Compounds were detected using a chromogenic ninhydrin spray.
D002855:Chromatography, Thin Layer / Q000379:methods*; D005688:Galactosamine / Q000032:analysis*; D005944:Glucosamine / Q000032:analysis*; D008070:Lipopolysaccharides / Q000032:analysis*; D010457:Peptidoglycan / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000032:analysis
D008070:Lipopolysaccharides; D010457:Peptidoglycan; D005688:Galactosamine; D005944:Glucosamine