Anticoagulant-free prepared blood platelets and the effect of calcium on their aggregation behaviour.
Human blood platelets were prepared by gel filtration of native blood and subsequent centrifugation in a Ficoll density gradient avoiding any anticoagulant during the preparation. In ADP, ionophore A 23 187 and thrombin induced aggregation an increase in extracellular Ca++ enhanced the velocity of aggregation. From a defined Ca++ concentration threshold on there was a shift from monophasic to biphasic aggregation.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000925:Anticoagulants; D001792:Blood Platelets; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology*; D002469:Cell Separation; D006801:Humans; D010974:Platelet Aggregation / Q000187:drug effects*; D013917:Thrombin / Q000494:pharmacology
D000925:Anticoagulants; D000001:Calcimycin; D000244:Adenosine Diphosphate; D013917:Thrombin; D002118:Calcium