Improved methods for the fluorographic detection of weak beta-emitting radioisotopes in Agarose and acrylamide gel electrophoresis media.
The use of acetic acid as a solvent for diphenyloxazole (PPO) in fluorographic procedures has been investigated. It is demonstrated to be superior to both dimethyl sulfoxide and methanol with respect to its suitability in both agarose and acrylamide gel electrophoresis systems. In addition, a method has been developed for impregnating fragile gels such as those used for immunodiffusion with PPO in preparation for fluorography.
D002250:Carbon Radioisotopes; D004279:DNA, Viral; D004121:Dimethyl Sulfoxide; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005453:Fluorescence; D005779:Immunodiffusion; D010080:Oxazoles; D011868:Radioisotopes / Q000032:analysis*; D012710:Serum Albumin, Bovine; D012997:Solvents; D014316:Tritium
D002250:Carbon Radioisotopes; D004279:DNA, Viral; D010080:Oxazoles; D011868:Radioisotopes; D012997:Solvents; D014316:Tritium; D012710:Serum Albumin, Bovine; C009012:2,5-diphenyloxazole; D004121:Dimethyl Sulfoxide