Presence of inhibin in testes of the Indian water buffalo Bubalus bubalis.
Isolation, purification and characterization of inhibin from the testes of the Indian buffalo Bubalus bubalis is described. The biological activity of the final product, which produced a single band on disc gel electrophoresis, was affected by heat treatment, 6 m urea, pH alteration and pepsin digestion. Chemically, it appeared to be a glycoprotein. The protein and carbohydrate components together accounted for a total of 63% of the dry weight, whereas 37% of the constituents were unknown.
D000818:Animals; D002020:Buffaloes / Q000378:metabolism*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D005640:Follicle Stimulating Hormone / Q000097:blood; D007265:Inhibins; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D011506:Proteins / Q000032:analysis*; D013735:Testicular Hormones / Q000032:analysis*; D013737:Testis / Q000032:analysis*
D011506:Proteins; D013735:Testicular Hormones; D007265:Inhibins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone