Estimation of energy metabolism in human skeletal muscle homogenate as a diagnostic aid.
A method is presented for the in vitro testing of the energy metabolism of human skeletal muscle. The levels of creatine phosphate, ATP, ADP and AMP, which are defined by the activity of many enzyme system, are measured after incubation of the muscle homogenate with various substrates in the presence or absence of specific inhibitors. Detection of abnormal metabolite concentrations is important for diagnosis of patients with myopathic syndromes.
D000227:Adenine Nucleotides / Q000494:pharmacology; D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D001151:Arsenic / Q000494:pharmacology; D018053:Arsenites; D002648:Child; D015226:Dinucleoside Phosphates; D004734:Energy Metabolism / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009132:Muscles / Q000378:metabolism*; D009135:Muscular Diseases / Q000175:diagnosis; D010725:Phosphocreatine / Q000378:metabolism
D000227:Adenine Nucleotides; D018053:Arsenites; D015226:Dinucleoside Phosphates; D010725:Phosphocreatine; D000249:Adenosine Monophosphate; C012275:P(1),P(5)-di(adenosine-5'-)pentaphosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; C015001:arsenite; D001151:Arsenic