title: Intestinal obstruction in germ-free dogs.
abstract: Mechanical occlusions were created in the intestines of four germ-free dogs. At the time of the operation, a control loop of mid-intestine was perfused in vivo and then excised for examinations in vitro, which included the determination of the equilibrium uptake of phenylalanine and of beta-methyl-glucoside, the influx kinetics of phenylalanine and morphometric analysis of the mucosa by microdissection and stereological techniques. Seven days after establishment of the occlusion the abdomen was reopened, and loops above and below the occlusion were perfused, and then excised for the same tests in vitro. Unlike occluded loops of conventional dogs, the intestine of the germ-free animal above the occlusion does not secrete water and electrolytes into the lumen. Its transport properties in vitro do not differ from those of the control loop, and the morphometric analyses reveal only slight changes in villus structure. The loop below the obstruction undergoes marked atrophy, as has been observed in conventional dogs. The results suggest that the copious secretion that occurs above an intestinal obstruction in normal animals is due to the presence of an abundant bacterial population in the obstruction fluid.
mesh_terms: D000818:Animals; D004285:Dogs; D005260:Female; D005856:Germ-Free Life; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D007415:Intestinal Obstruction / Q000378:metabolism* / Q000473:pathology; D007421:Intestine, Small / Q000473:pathology; D008297:Male; D008757:Methylglucosides / Q000378:metabolism; D010649:Phenylalanine / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D014867:Water / Q000378:metabolism
chemical_list: D008757:Methylglucosides; D014867:Water; D010649:Phenylalanine; C027020:methylglucoside; D012964:Sodium; D005947:Glucose; D011188:Potassium