Metabolism of arachidonate through NADPH-dependent oxygenase of renal cortex.
In normal kidneys the renal medulla very efficiently converts arachidonic acid to prostaglandins. Although the renal cortex has only trace amounts of cyclooxygenase activity, we report here the existence of an active cortical NADPH-dependent monooxygenase that converts arachidonate primarily into 19-hydroxy- and 20-hydroxyarachidonate as well as 19-ketoarachidonate and a dicarboxylic acid. The enzyme is presumably a cytochrome P-450 monooxygenase and demonstrated marked resistance to inhibition by 2-diethylaminoethyl-2,2-diphenylvalerate hydrochloride (SKF-525A), metyrapone, and carbon monoxide. In the rabbit kidney these products are produced only by the cortex in the presence of NADPH and represent the major metabolic products of arachidonate metabolism.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D001189:Aryl Hydrocarbon Hydroxylases; D000072467:Cytochrome P450 Family 2; D008401:Gas Chromatography-Mass Spectrometry; D007672:Kidney Cortex / Q000378:metabolism*; D008297:Male; D006899:Mixed Function Oxygenases; D010105:Oxygenases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011817:Rabbits
D001095:Arachidonic Acids; D016718:Arachidonic Acid; D006899:Mixed Function Oxygenases; D010105:Oxygenases; D001189:Aryl Hydrocarbon Hydroxylases; C033914:Cyp2b19 protein, mouse; D000072467:Cytochrome P450 Family 2