Mutation induction in a radiation-sensitive variant of mammalian cells.
Ultraviolet light (UV)-induced mutations were compared between a UV-sensitive variant and its parental mouse lymphoma cell line. The variant was originally isolated for its sensitivity to 4-nitro-quinoline-1-oxide by employing a replica plating method and proved to be sensitive to the killing by UV as well. Caffeine potentiation of UV killing was observed to a similar extent in the variant and the parental cells, indicating that the caffeine-insensitive process is responsible for UV sensitivity in the variant. The induced mutation frequency as determined by resistance to 6-thioguanine was higher in the variant than in the parental cells per unit dose of UV as well as at the comparable survival level.
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D002999:Clone Cells / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D014644:Genetic Variation; D007940:Leukemia L5178; D051379:Mice; D009154:Mutation; D011836:Radiation Tolerance; D014466:Ultraviolet Rays
D015112:4-Nitroquinoline-1-oxide