Electron dense granules and the role of buffers: artefacts from fixation with glutaraldehyde and osmium tetroxide.
Electron dense granules may appear in tissues after glutaraldehyde prefixation and osmium tetroxide postfixation. In order to determine the conditions under which the granules are formed various vehicles in pre- and post-fixatives were tested on lymph node, thymus and heart. If granules appeared they were found in all cell types investigated, particularly in the nuclei. There was no difference in the distribution of the granules in the different compartments of these organs. The granules probably consist of complexes of glutaraldehyde, osmium and phosphate. The ultimate phosphate concentration in the tissues during the postfixation was shown to determine whether or not the artefacts appeared. Local conditions in the tissues also contributed to the appearance of the granules. It is concluded that phosphate buffers can be used in the double fixation procedure, but to avoid the granules in lymph node, thymus and heart, a concentration of 0.1 mol/l or less phosphate should be used. For brain and kidney other conditions apply.
D000818:Animals; D002021:Buffers; D005404:Fixatives; D005976:Glutaral; D008198:Lymph Nodes / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009206:Myocardium / Q000648:ultrastructure; D009993:Osmium Tetroxide; D010710:Phosphates; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000648:ultrastructure
D002021:Buffers; D005404:Fixatives; D010710:Phosphates; D009993:Osmium Tetroxide; D005976:Glutaral