Cloning of the Bacillus subtilis sulfanilamide resistance gene in Bacillus subtilis.
A recombinant plasmid was constructed by ligation of chromosomal DNA from a sulfanilamide-resistant strain of Bacillus subtilis to the plasmid vector pUB110 which specifies neomycin resistance. Recombinant molecules generated in vitro were introduced into a B. subtilis recipient strain which carried the recE4 mutation, and selection was for neomycin-sulfanilamide-resistant transformants. A single colony was isolated containing the recombinant plasmid pKO101. This 6.3-megadalton plasmid simultaneously conferred resistance to neomycin and sulfanilamide when transferred into sensitive Rec+ or Rec- cells by either transduction or transformation.
D001412:Bacillus subtilis / Q000235:genetics*; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004352:Drug Resistance, Microbial; D005798:Genes, Bacterial; D009355:Neomycin; D010957:Plasmids; D013424:Sulfanilamides / Q000494:pharmacology*; D014169:Transformation, Bacterial
D004274:DNA, Recombinant; D013424:Sulfanilamides; D009355:Neomycin