Structures of heterooligosaccharides synthesized by levansucrase.
In the presence of excess amounts of various monosaccharides as acceptors, Bacillus subtilis levansucrase synthesized various heterooligosaccharides as a result of transfer of the fructosyl residue from sucrose. The saccharides produced in the presence of D-glucose, D-mannose, and D-xylose were all non-reducing disaccharides and were identified as beta-D-fructofuranosyl-alpha-D-glucopyranoside (sucrose), beta-D-fructo-furanosyl-alpha-D-mannopyranoside (mannosucrose), and beta-D-fructofuranosyl-alpha-D-xylopyranoside (xylsucrose), respectively. The saccharides produced in the presence of D-galactose were also non-reducing, but consisted of di-, tri-, and tetrasaccharides. The di- and trisaccharides were galsucrose and 6F-beta-D-fructofuranosyl-galsucrose. The saccharide synthesized in the presence of L-arabinose was a reducing saccharide and was determined to be 4-O-beta-D-fructofuranosyl-L-arabinose. In the presence of D-fructose, several reducing levan oligomers, levanbiose, levantriose, levantetraose, etc., were produced, which were not observed in the synthesis of levan from sucrose alone.
D001089:Arabinose / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology*; D055598:Chemical Phenomena; D002621:Chemistry; D005630:Fructans / Q000096:biosynthesis; D005632:Fructose / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005959:Glucosidases; D006602:Hexosyltransferases / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D009844:Oligosaccharides / Q000096:biosynthesis*; D013395:Sucrose / Q000378:metabolism; D014994:Xylose / Q000378:metabolism
D005630:Fructans; D009844:Oligosaccharides; D005632:Fructose; D013395:Sucrose; D014994:Xylose; D001089:Arabinose; D006602:Hexosyltransferases; C021513:levansucrase; D005959:Glucosidases; D005947:Glucose; D008358:Mannose; D005690:Galactose