A human plasma cell line: induction and characterization.
A stable line of IgG K producing human plasma cells was established from a myelomatous human bone marrow using conditioned media from a rapidly metabolizing lymphoblast line, RPMI 4098. Growth in RPMI 1640 (15% fetal calf serum) at 6% CO2 promoted a 62-hour doubling time with a preferred cell concentration of 1 x 10(6)/mL. Surface marker studies showed: no receptors for sheep erythrocytes, no surface immunoglobulins, variable number of cells bearing complement receptors and 83% bearing Fc receptors. Although transmission electron micrographs demonstrated a poorly developed endoplasmic reticulum, radioimmunoassay showed 23 ng IgG and 28.7 ng Kappa were produced by 1 x 10(6) cells in 72 hours. Further, the cells are lipase, esterase and Epstein-Barr nuclear antigen negative. ASG banding showed a total chromosome number that varied from 46--49. Since the number of human plasma cell lines is limited, it is felt that this line will augment the immunobiological study of human myeloma.
D001853:Bone Marrow / Q000473:pathology*; D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002871:Chromosome Banding; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007145:Immunoglobulin kappa-Chains / Q000032:analysis; D009101:Multiple Myeloma / Q000276:immunology / Q000473:pathology*; D010950:Plasma Cells / Q000276:immunology / Q000648:ultrastructure; D010954:Plasmacytoma / Q000276:immunology; D011951:Receptors, Complement / Q000032:analysis; D011961:Receptors, Fc / Q000032:analysis
D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D011951:Receptors, Complement; D011961:Receptors, Fc