title: [Effect of 2 methods of demineralization on the on the preservation of glycoproteins and proteoglycans in the intertubular and peritubular dentin in the horse].
abstract: The effect of 2 methods of demineralization on the preservation of proteoglycans and glycoproteins was studied in the intertubular and peritubular dentine of the horse. The specimens embedded in Epon were demineralized with a 2% acid formic solution (Bonucci and Gheradi, 1975). Other fragments were treated with an organic solution of EDTA alkylammonium salt (Scott and Kyffin, 1979). These methods preserved in a satisfactory way these labile organic components. In the intertubular dentine, glycoproteins and proteoglycans were also identified, either associated with collagen fibres as a glue and granules, or present in the interfibrillar spaces. In the peritubular dentine, amorphous material formed a fine network between the mineral structures.
mesh_terms: D000818:Animals; D003094:Collagen; D003461:Crystallography; D003648:Decalcification Technique; D003804:Dentin / Q000648:ultrastructure*; D004492:Edetic Acid / Q000494:pharmacology; D005561:Formates / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism; D006736:Horses; D011509:Proteoglycans / Q000378:metabolism
chemical_list: D005561:Formates; D006023:Glycoproteins; D011509:Proteoglycans; D003094:Collagen; D004492:Edetic Acid
vernacular_title: Effets de deux méthodes de déminéralisation sur la préservation des glycoprotéines et des  protéoglyccanes dans les dentines intercanaliculaires et péricanaliculaires chez le cheval.