Genetical tests for the frequency of small deletions among ems-induced point mutations in Drosophila.
In order to estimate the proportion of small deletions among EMS-induced point mutations we scored visible mutations at 6 sex-linked loci either by a specific-locus test, in which both deletions and intragenic changes survive, or in sons of attached-X females, in which deletions do not survive. About twice as many visibles were detected in the specific-locus as in the attached-X test, and between 50 and 90% of the former were lethal to males. From this we have concluded that at least 60% of EMS-induced point mutations are small deletions. The high ratio of lethal to viable visible mutations was in agreement with this conclusion. These results are compared with data from the literature, most of which report very low deletion frequencies among EMS-induced mutations. The alternative possibility, namely that EMS tends to produce clusters of linked mutations, has also been considered and finds some support in literature. Whatever the cause of the high frequency of lethals associated with specific visible mutations, our data suggest that genetic hazards from EMS may be considerable; this is indeed true for its effect of the viability of heterozygotes.
D000818:Animals; D002872:Chromosome Deletion; D002875:Chromosomes / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D004331:Drosophila melanogaster / Q000235:genetics*; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005804:Genes, Lethal; D009152:Mutagenicity Tests; D009153:Mutagens
D009153:Mutagens; D005020:Ethyl Methanesulfonate