Cellular and subcellular localization of protein I in the peripheral nervous system.
The cellular and subcellular distribution of protein I, a major brain phosphoprotein, has been studied in the peripheral nervous system. The levels of protein I in various peripheral nerves and innervated peripheral tissues were determined by radioimmunoassay and radioimmunolabeling of polyacrylamide gels. The results indicated tha protein I is present throughout the peripheral nervous system. Denervation studies of adrenal medulla and iris suggested that the protein I contained in peripheral tissues is localized to the neuronal elements innervating those tissues. Protein I was found to be enriched in neurotransmitter vesicle fractions of peripheral nervous tissue. Moreover, protein I appeared to be transported from cell bodies to axons terminals at least partly in association with neurotransmitter vesicles.
D000311:Adrenal Glands / Q000294:innervation; D000313:Adrenal Medulla / Q000032:analysis; D000818:Animals; D003714:Denervation; D004299:Dopamine beta-Hydroxylase / Q000032:analysis; D007498:Iris / Q000032:analysis / Q000294:innervation; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009638:Norepinephrine / Q000032:analysis; D010525:Peripheral Nerves / Q000032:analysis*; D010750:Phosphoproteins / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats; D013347:Subcellular Fractions / Q000032:analysis; D016704:Synapsins
D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D016704:Synapsins; D004299:Dopamine beta-Hydroxylase; D009638:Norepinephrine