Increased frequency of late pre- beta lipoproteins (LP beta) in isolated serum very low density lipoproteins in uraemia.
Hypertriglyceridaemia, which is caused by raised levels of the triglyceride rich serum very low density lipoproteins (VLDL), is common in uraemia. Here we report qualitative studies on VLDL in this condition. VLDL were isolated from serum of 137 uraemic patients by ultracentrifugation, subjected to agarose electrophoresis and stained for lipids. An increased frequency of presence of electrophoretically slow migrating, discrete VLDL band, the so-called late pre- beta lipoproteins, LP beta, was found, 76% in uraemia versus 30% in controls. Patients on haemodialysis had a higher frequency of LP beta than those not on dialysis, 94% versus 69%. Furthermore the cholesterol/triglyceride ratio of VLDL was raised by about 30% in uraemia. Since the cholesterol enriched LP beta is believed to represent an end stage of the catabolism of VLDL these data suggest that there is a delayed clearance of VLDL remnants in uraemia. In haemodialysis patients heparin might have contributed to the higher frequency of LP beta.
D002784:Cholesterol / Q000097:blood; D004586:Electrophoresis; D006801:Humans; D008079:Lipoproteins, VLDL / Q000032:analysis / Q000097:blood*; D006435:Renal Dialysis; D014280:Triglycerides / Q000097:blood; D014511:Uremia / Q000097:blood*
D008079:Lipoproteins, VLDL; D014280:Triglycerides; D002784:Cholesterol