Intravenous arachidonate-induced respiratory distress: comparative study of three in vivo models for the measurement of antithrombotic activity.
Three different in vivo methods for the study of antithrombotic drugs have been compared. A syndrome of acute respiratory distress has been induced by intravenous injection of arachidonate in three different animal species: mouse, rabbit, and hamster. The intensity of the induced stress was quantified by different procedures: visual observation in the mouse, death or survival in the rabbit, and mechanical recording of the respiration in the hamster. The protective effect of a new antithrombotic agent, triflusal, a salicylic acid derivative, was studied. At the doses used, triflusal has no effect on the respiratory center activity of control animals. The results show that a new method using a mechanical device to record the intensity and duration of the respiratory distress in hamsters is the most suitable of the three methods studied.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology*; D006224:Cricetinae; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D007275:Injections, Intravenous; D008297:Male; D008647:Mesocricetus; D051379:Mice; D011817:Rabbits; D012140:Respiratory Tract Diseases / Q000139:chemically induced* / Q000503:physiopathology; D013045:Species Specificity; D013927:Thrombosis / Q000139:chemically induced*
D001095:Arachidonic Acids; D005343:Fibrinolytic Agents; D016718:Arachidonic Acid