Effects of fluoride, calcium, and phosphate administration on mineralization in rats.
Seven days before a fluoride injection of 20 mg sodium fluoride per kg body weight, 3-month-old rats grown on a standard pellet diet containing 0.8% calcium and 1.4% phosphate were given a diet of rice with only 0.025% calcium and 0.1% phosphate. Microradiographs of the continuously growing incisors showed a hypermineralized and subsequent hypomineralized zone. Blood analysis demonstrated a decrease and a subsequent reestablishment of plasma calcium concentration. In some experiments calcium and phosphate were administered to compensate the hypocalcemia which prevented the hypomineralized zone from arising. A delay of calcium and phosphate administration led to formation of a mineralized band within the hypomineralized zone. The results are discussed with reference to calcium homeostasis.
D000284:Administration, Oral; D000818:Animals; D002118:Calcium / Q000097:blood / Q000494:pharmacology*; D003804:Dentin / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005459:Fluorides / Q000097:blood / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D008297:Male; D010710:Phosphates / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D012969:Sodium Fluoride / Q000008:administration & dosage; D014070:Tooth / Q000648:ultrastructure; D014074:Tooth Calcification / Q000187:drug effects*
D010710:Phosphates; D012969:Sodium Fluoride; D005459:Fluorides; D002118:Calcium