In vitro synthesis of a mutagenic azide metabolite by cell-free bacterial extracts.
Cell-free extracts of Salmonella typhimurium synthesize a mutagenic azide metabolite from sodium azide and O-acetylserine. S. typhimurium mutant DW379 (O-acetylserine sulfhydrylase-deficient) extracts were neither able to carry out this reaction not produce the mutagenic azide metabolite in vivo. The in vitro reaction was inhibited by sulfide but not by L-cysteine. The catalytic activity responsible for the mutagenic metabolite synthesis was stable to brief heating up to 55 degrees C and had a pH optimum between 7-7.4. These results suggest that the enzyme O-acetylserine sulfhydrylase catalyzes the reaction of azide with O-acetylserine to form a mutagenic azide metabolite.
D001386:Azides / Q000378:metabolism* / Q000494:pharmacology; D001711:Biotransformation; D009153:Mutagens / Q000378:metabolism*; D009154:Mutation; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism*; D012694:Serine / Q000378:metabolism
D001386:Azides; D009153:Mutagens; D012694:Serine