X-ray absorption edge spectroscopy of Co(II)-binding sites of copper- and zinc-containing proteins.
X-ray absorption near-edge spectroscopy (XANES) of Co(II) in three derivatives of superoxide dismutase, namely [Cu(II)-Co(II)], [Cu(I)-Co(II)] and [...-Co(II)], suggests a tetrahedral coordination of the metal for all compounds. Significant differences, detected in the spectrum of the [Cu(II)-Co(II)] derivative as compared to the other species, indicate that a conformational change and/or a different charge of the imidazole bridging the two metal sites in superoxide dismutase occur in coincidence with the change of copper valence. The XANES spectra of the cobalt derivatives of alcohol dehydrogenase, carbonic anhydrase and stellacyanin show features that can be accounted for by an increasing degree of covalency in the metal first sphere of coordination, in the following order: alcohol dehydrogenase greater than stellacyanin greater than superoxide dismutase greater than or equal to carbonic anhydrase.
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D001665:Binding Sites; D002256:Carbonic Anhydrases / Q000378:metabolism; D003035:Cobalt; D003300:Copper / Q000032:analysis*; D008667:Metalloproteins / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D013057:Spectrum Analysis; D013482:Superoxide Dismutase / Q000378:metabolism; D014965:X-Rays; D015032:Zinc / Q000032:analysis*
D008667:Metalloproteins; D010940:Plant Proteins; C003343:stellacyanin protein, plant; D003035:Cobalt; D003300:Copper; D000429:Alcohol Oxidoreductases; D013482:Superoxide Dismutase; D002256:Carbonic Anhydrases; D015032:Zinc