Measurement of IgM antibodies in the diagnosis of mycoplasma pneumonia.
The sera of 30 patients with complement-fixing antibodies to Mycoplasma pneumoniae (titres larger than or equal to 32) were treated with staphylococcal protein A. This procedure effectively removed 90 to 95% of the IgG antibodies. The Mycoplasma-specific antibodies in the treated sera could then be measured by a complement fixation test. This provides a useful test for distinguishing serological responses to recent Mycoplasma pneumoniae infections from anamnestic responses due to past exposure to Mycoplasma pneumoniae.
D000373:Agglutinins / Q000032:analysis; D000907:Antibodies, Bacterial / Q000032:analysis*; D003080:Cold Temperature; D003168:Complement Fixation Tests; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis*; D009177:Mycoplasma pneumoniae / Q000276:immunology*; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis* / Q000276:immunology; D013205:Staphylococcal Protein A
D000373:Agglutinins; D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013205:Staphylococcal Protein A