An investigation of the clonality of human autoimmune thyroglobulin antibodies and their light chains.
Thyroglobulin antibodies in the sera from 31 patients with a variety of disorders were studied by isoelectric focusing. Only one gave a spectrotype indicative of a monoclonal response, the other 30 giving spectrotypes characteristic of polyclonal responses. There was evidence of clonal dominance in some of the sera and each gave a different spectrotype. Light chains were prepared from five thyroglobulin antibodies purified by affinity chromatography. There was no restriction in the spectrotypes when compared with light chains prepared from normal immunoglobulin.
D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001323:Autoantibodies / Q000235:genetics / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D005802:Genes, MHC Class II; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000235:genetics / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D013954:Thyroglobulin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D007147:Immunoglobulin Light Chains; D013954:Thyroglobulin