Interaction of o,p'-DDT with the estrogen-binding protein (EBP) of DMBA-induced rat mammary tumors.
The effect of o,p'-DDT on the binding of 3H-estradiol to DMBA-induced rat mammary tumor cytosolic estrogen-binding protein (EBP) was examined in vitro. Scatchard plot analysis indicated that o,p'-DDT competitively inhibited 3H-estradiol binding and sucrose density gradient analysis demonstrated that o,p'-DDT displaced estradiol from specific 4S and 8S proteins. As estrogens have been shown to affect the development and growth of these tumors, our experimental findings suggest that o,p'-DDT may possibly influence DMBA-induced tumors in an estrogenic manner.
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D003634:DDT / Q000494:pharmacology*; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism* / Q000648:ultrastructure; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D011960:Receptors, Estrogen
D002352:Carrier Proteins; D004967:Estrogens; D011960:Receptors, Estrogen; C010140:estrophilin; D015127:9,10-Dimethyl-1,2-benzanthracene; D003634:DDT; C016340:o,p'-DDT