[Preparation of specific pituitary cells by centrifugal elutriation].
The cells of the male rat pituitary were enzymatically dispersed with trypsin and then separated by centrifugal elutriation. The commercial equipment requires minor modifications, especially the infection system. Using a gradient of flow, enriched gonadotrop cells are obtained. The results compare favourably with those obtained by sedimentation at unit gravity.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation / Q000379:methods; D008297:Male; D010903:Pituitary Gland, Anterior / Q000166:cytology*; D051381:Rats
Préparation de cellules hypophysaires spécifiques par élutriation centrifuge.