title: Induction of citric acid cycle enzymes during initiation of sporulation by guanine nucleotide deprivation.
abstract: In Bacillus subtilis, conditions causing partial deprivation of guanine nucleotides initiated sporulation and caused the synthesis of citrate synthase, aconitase, and alpha-ketoglutarate dehydrogenase. Alpha-ketoglutarate dehydrogenase could also be induced by acetate, and the specific activity of this enzyme was elevated in mutants that had high intracellular acetyl coenzyme A concentrations because they lacked citrate synthase activity. After deprivation of guanine nucleotides, the intracellular concentration of acetyl coenzyme A also increased, which explained the induction of alpha-ketoglutarate dehydrogenase. Furthermore, the decreases in alpha-ketoglutarate and L-malate concentrations observed during this deprivation accounted for the observed increases in citrate synthase activity (which was repressed by alpha-ketoglutarate and malate) and aconitase activity (which was repressed by alpha-ketoglutarate).
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000105:Acetyl Coenzyme A / Q000378:metabolism; D000154:Aconitate Hydratase / Q000096:biosynthesis; D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D001412:Bacillus subtilis / Q000201:enzymology*; D002950:Citrate (si)-Synthase / Q000096:biosynthesis; D002952:Citric Acid Cycle; D004790:Enzyme Induction / Q000187:drug effects; D006150:Guanine Nucleotides / Q000494:pharmacology*; D007655:Ketoglutarate Dehydrogenase Complex / Q000096:biosynthesis; D007656:Ketoglutaric Acids / Q000378:metabolism; D009154:Mutation; D011773:Pyruvates / Q000378:metabolism; D013171:Spores, Bacterial
chemical_list: D000085:Acetates; D006150:Guanine Nucleotides; D007656:Ketoglutaric Acids; D011773:Pyruvates; C012182:angustmycin A; D000105:Acetyl Coenzyme A; D007655:Ketoglutarate Dehydrogenase Complex; D002950:Citrate (si)-Synthase; D000154:Aconitate Hydratase; D000241:Adenosine