Chromatographic and biologic analysis of ME and OVLT LHRH.
The luteinizing hormone (LH)-releasing activities a pooled rat organum vasculosum lamina terminalis (OVLT) and median eminence (ME) tissues were evaluated for chromatographic and biologic similarity and compared to those of synthetic decapeptide LH-releasing hormone (LHRH). The LHRH detected in these extracts appeared similar chromatographically (Sephadex G-25) to synthetic LHRH. These extracts, as well as synthetic LHRH, were also capable of stimulating dose dependent gonadotropin release form cultures rat gonadotrophs. These findings suggest a physiological role of the LHRH present in the rat OVLT in the control of gonadotropin secretion.
D000818:Animals; D001681:Biological Assay; D001923:Brain Chemistry; D002478:Cells, Cultured; D002552:Cerebral Ventricles / Q000032:analysis*; D002850:Chromatography, Gel; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis*; D007030:Hypothalamo-Hypophyseal System / Q000032:analysis*; D007031:Hypothalamus / Q000032:analysis*; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D008473:Median Eminence / Q000032:analysis*; D009928:Organ Specificity; D010903:Pituitary Gland, Anterior / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats
D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone