A three step purification procedure for human liver ferritin.
We describe a method for the purification of normal human liver ferritin by ultrafiltration, gel filtration on Sephacryl S-300, and affinity chromatography on DEAE-Affi Gel Blue. The purity of the ferritin obtained was verified by immunoelectrophoresis, Ouchterlony immuno-diffusion, polyacrylamide gel electrophoresis, and electrofocusing. This rapid method yields 32% of the original ferritin.
D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005293:Ferritins / Q000302:isolation & purification*; D006736:Horses; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing; D008099:Liver / Q000032:analysis*; D008722:Methods; D008970:Molecular Weight; D014462:Ultrafiltration
D005293:Ferritins