Prevention by estradiol of aflatoxin-induced cytotoxicity in cultured chick embryo liver cells.
Aflatoxin B1 at a final concentration of 10(-5) M depressed the sytheses of DNA, RNA and protein in cultured chick embryo liver cells. The same dose produced ultrastructural changes in the nucleus, such as nucleolar compactness or segregation of fibrillar and granular components and in the cytoplasmic area, such as vesiculation, dilatation or degranulation of endoplasmic meticulum. These acute toxic effects of aflatoxin B1 were partially decreased by an addition of 4 X 10(-5) M estradiol-17 beta. Namely estradiol-17 beta significantly reduced the nucleolar compactness and segregation of fibrillo-granular components but did not improve the vesiculation, dilatation and degranulation of endoplasmic reticulum. Estradiol-17 beta also protected the liver cells from the aflatoxin B1-induced inhibition of nucleic acid and protein synthesis. These results suggest that the protective effect of estradiol-17 beta against the acute hepatotoxicity of aflatoxin B1 is mainly due to an antagonistic interaction of both compounds on the synthesis of nucleic acid in nucleolus.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000037:antagonists & inhibitors*; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004958:Estradiol / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D009963:Orotic Acid / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D000348:Aflatoxins; D004958:Estradiol; D009963:Orotic Acid; D016604:Aflatoxin B1; D007930:Leucine; D013936:Thymidine