The inhibition of DNA repair by aphidicolin or cytosine arabinoside in X-irradiated normal and xeroderma pigmentosum fibroblasts.
Normal and excision-deficient xeroderma pigmentosum fibroblasts were X-irradiated and the influence on DNA repair of either the repair inhibitor cytosine arabinoside or the specific inhibitor of Dna polymerase alpha, aphidicolin, investigated. The data indicated that the repair of a certain fraction of X-ray-induced lesions can be inhibited in both cell lines by both compounds. Thus, as aphidicolin blocks the operation of polymerase alpha, this enzyme must be involved in an excision repair pathway operating in both normal and excision-deficient xeroderma pigmentosum cells.
D016590:Aphidicolin; D002478:Cells, Cultured; D003561:Cytarabine / Q000494:pharmacology*; D004247:DNA / Q000528:radiation effects; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors / Q000502:physiology; D004260:DNA Repair / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D014983:Xeroderma Pigmentosum / Q000235:genetics*
D004224:Diterpenes; D003561:Cytarabine; D016590:Aphidicolin; D004247:DNA; D004257:DNA Polymerase II