title: Protease modification enhances anti-D binding to nucleated red blood cell precursors.
abstract: Protease modification of nucleated red blood cell precursors resulted in significant enhancement of anti-D binding, indicating the presence of cryptic Rh determinants within the plasma membrane of the developing erythroid cell. Protease-modified nucleated precursor cells bound less anti-D than protease-modified mature red blood cells, however, suggesting that the total content of D antigen in the developing erythropoietic plasma membrane is reduced. These findings support the hypothesis that the D antigen, an integral membrane protein, is progressively synthesized during erythropoiesis.
mesh_terms: D004910:Erythrocyte Membrane / Q000032:analysis; D004912:Erythrocytes / Q000166:cytology / Q000276:immunology*; D004920:Erythropoiesis; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis; D010447:Peptide Hydrolases / Q000494:pharmacology*; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
chemical_list: D008565:Membrane Proteins; D012204:Rh-Hr Blood-Group System; D010447:Peptide Hydrolases