Isolation and characterization of a myeloma--spleen-cell hybrid producing antibody to phenylalanine hydroxylase.
Application of the technique of myeloma--spleen-cell fusion [Kohler & Milstein (1975) Nature (London) 256, 495--497] has allowed the isolation of a cell colony that produced a monoclonal antibody against monkey liver phenylalanine hydroxylase. The antibody exhibited cross-reactivity against hepatic phenylalanine hydroxylase from other mammalian species, including human, rat and mouse. Cross-reactivity was established by (a) enzyme-inhibition assay, (b) double-immunodiffusion reaction, and (c) two-dimensional polyacrylamide-gel-electrophoretic analysis of immunoprecipitate. The various properties of the monoclonal antibody and its use in the study of mammalian phenylalanine hydroxylase are presented.
D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D003429:Cross Reactions; D000882:Haplorhini; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology*; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D007621:Karyotyping; D008099:Liver / Q000201:enzymology; D051379:Mice; D009101:Multiple Myeloma / Q000276:immunology*; D010651:Phenylalanine Hydroxylase / Q000276:immunology*; D051381:Rats; D013154:Spleen / Q000276:immunology*
D010651:Phenylalanine Hydroxylase