[Effect of methotrexate on oxygen consumption of L1210 cells and their isolated mitochondria].
Methotrexate, at doses inhibiting cell growth without any lethal effect, alters oxygen consumption in L 1210 cells as soon as 3 hrs. after culture. After a 24 hrs. treatment with methotrexate, there is a close relationship between cellular oxygen uptake and growth inhibition. These two effects are reversible. With mitochondria from treated cells, an inhibition of respiration is observed in the presence of glutamate-malate. No effect occurs with succinate. These results in vitro are compatible with concentrations used in patients.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D007700:Kinetics; D007939:Leukemia L1210 / Q000378:metabolism*; D007945:Leukemia, Lymphoid / Q000378:metabolism; D008727:Methotrexate / Q000494:pharmacology*; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects*
D008727:Methotrexate
Effets du méthotrexate sur la consommation d'oxygène des cellules L1210 et de leurs mitochondries isolées.