Rabbit anti-human CSF IgG. I. Characterization of anti-idiotype antibodies produced against MS CSF and detection of cross-reactive idiotypes in several MS CSF.
Serum from rabbits injected with IgG prepared from the CSF of patients with multiple sclerosis were rendered anti-idiotypic by adsorption on sequential columns of pooled human gamma-globulin, free light chains and free heavy chains. Adsorbed antisera precipitated between 15% and 45% of the autologous ligand and were inhibited by autologous CSF and serum and F(ab')2 fragments prepared from serum IgG. Idiotypic cross-reactivities were detected among the CSF IgG of several MS patients using three of these antisera. Two antisera demonstrated low levels of cross-reactivity with CSF IgG obtained from two of six heterologous MS patients. The third antiserum detected highly cross-reactive idiotypic determinants in the CSF IgG of two family members both having MS.
D000328:Adult; D000368:Aged; D000818:Animals; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D003429:Cross Reactions; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid* / Q000276:immunology; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes / Q000134:cerebrospinal fluid* / Q000276:immunology; D007147:Immunoglobulin Light Chains; D007163:Immunosorbent Techniques; D007525:Isoelectric Focusing; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid / Q000276:immunology*; D011817:Rabbits
D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007147:Immunoglobulin Light Chains