[Synthesis of a lens-specific antigen (delta-crystallin) in rudimentary chicken adenohypophysis].
The synthesis of two lens-specific proteins, delta- and beta-crystallins, by adenohypophyseal anlage of 4-day chick embryos was studied by the immunofluorescence technique in conjunction with autoradiography. Isolated anlages were incubated for 16 hours in a culture medium containing 14c-leucine. The synthesis was determined with the use of an unlabelled carrier, extract of chick lens, as well as of antisera against delta- and beta-crystallins. 14C-Leucine incorporation was found to occur only in delta-crystalline precipitation line rather than in beta-crystallin line. This evidence attests to the synthesis of delta-crystalline by the chick embryo adenohypophyseal anlage. The results are in agreement with the previously obtained immunohistochemical data on delta-crystalline localization in cells of the developing adenohypophysis.
D000818:Animals; D000941:Antigens / Q000032:analysis; D001345:Autoradiography; D002642:Chick Embryo; D003459:Crystallins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D009928:Organ Specificity; D010903:Pituitary Gland, Anterior / Q000196:embryology / Q000378:metabolism*
D000941:Antigens; D003459:Crystallins
Sintez spetsificheskogo antigena krustalika (delta-kristallina) v zachatke adenogipofiza kus.