Inhibitors of repair DNA synthesis.
We have measured repair DNA synthesis in UV-irradiated normal human fibroblasts, grown to a defined state of quiescence in order to avoid the problem of discriminating repair from replicative DNA synthesis. We have assessed the effects of various DNA synthesis inhibitors on repair. Inhibition of repair synthesis by hydroxyurea, 1-beta-D-arabinofuranosylcytosine and aphidicolin is associated with the ability to accumulate DNA breaks due to enzymic incision at DNA damage sites; the inhibition by novobiocin is in accord with its known ability to block incision.
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D016590:Aphidicolin; D002460:Cell Line; D003561:Cytarabine / Q000494:pharmacology; D004260:DNA Repair; D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects*; D004224:Diterpenes / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D007700:Kinetics; D009675:Novobiocin / Q000494:pharmacology; D014466:Ultraviolet Rays
D000903:Antibiotics, Antineoplastic; D004224:Diterpenes; D003561:Cytarabine; D009675:Novobiocin; D016590:Aphidicolin; D006918:Hydroxyurea