Control of large-scale plasma thawing for recovery of cryoprecipitate factor VIII.
Cryoprecipitation is commonly used as the primary step in the preparation of clinical factor VIII concentrates; yet recovery is usually very low. Much of this loss is due to poor temperature control and a process of continuous plasma thawing has been designed to overcome this. A substantial improvement has resulted, with an increase in both yield and purity of factor VIII:C of over 50% in comparison to a conventional batch thaw process.
D000941:Antigens / Q000032:analysis; D011232:Chemical Precipitation; D005169:Factor VIII / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification / Q000627:therapeutic use; D005615:Freezing; D006801:Humans; D007158:Immunologic Techniques; D010949:Plasma; D012995:Solubility; D013696:Temperature; D013997:Time Factors; D014841:von Willebrand Factor
D000941:Antigens; D014841:von Willebrand Factor; D005169:Factor VIII