The glycoprotein composition of peripheral nervous system myelin subfractions.
Two fractions were isolated by continuous density gradient centrifugation from total particulate matter of rabbit sciatic nerves: a minor fraction, B, consisting of small-sized membrane fragments and a major fraction, C, of characteristic multilayered myelin figures, with maxima at 0.33 and 0.58 M-sucrose, respectively. In comparison with C, fraction B was enriched in CNPase and alkaline phosphatase activities and the P0, 23K and Z proteins, but was virtually devoid of basic protein. The glycoprotein composition of all fractions was examined with four fluorescein isothiocyanate-labelled lectins (WGA, Con A, RCA-60, U.E.). These revealed the presence of six glycoproteins in all fractions with similar lectin binding capacities and molecular weights ranging from 35,500 to 16,000, of which P0 was the predominant component. Material found on the heavy side of fraction C was characterized by the presence of a multitude of glycoproteins which bound variable proportions of the four different lectins, suggesting substantial variations in their carbohydrate moieties. Their absence from the central portion of fraction C points to a location other than that of compact PNS myelin.
D000818:Animals; D002501:Centrifugation, Zonal; D004591:Electrophoresis, Polyacrylamide Gel; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006023:Glycoproteins / Q000032:analysis*; D037102:Lectins; D008854:Microscopy, Electron; D008970:Molecular Weight; D009185:Myelin Proteins / Q000032:analysis*; D010525:Peripheral Nerves / Q000032:analysis*; D011817:Rabbits; D013861:Thiocyanates
D005452:Fluoresceins; D006023:Glycoproteins; D037102:Lectins; D009185:Myelin Proteins; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate