title: Mutations induced by a hormonal imbalance in Drosophila melanogaster.
abstract: Pterin treatment of chrysalids in diapause modified the juvenile hormone- ecdysone ratio. The treatment of Drosophila donor mutant with a mixture of reduced folic acid, pterins and extract of Pierides chrysalids in diapause induced the formation of short sequences, by the intermediary of variations in hormonal balance. The effect of this variation was seen in the germinal lines especially at the gonial stage, where recessive, visible or lethal mutations were induced in the form of clusters. Genetically active fractions were found in the 4S-8S and 18S-28S sedimentation zones after saccharose gradient ultracentrifugation. The short DNA sequences coded for tRNA and rRNA. Consequently, it is there DNA sequences from the Drosophila donor mutant that altered the genetic information of the host. 2 types of recessive visible mutation appeared: those affecting the differentiation of the imaginal discs and those affecting the pigment biosynthetic chain. Recessive lethals were induced by treatment. 3 hypothesis are proposed: the first suggests the formation of a short DNA sequence complexing at a specific locus in the acceptor. The second involves transposable factors belonging to the acceptor itself, behaving as a particular transposable factor. The third supposes the induction of alterations at loci of rRNA and tRNA synthesis at the origin of perturbations in protein synthesis. The present data do not allow us to choose between the 3 hypotheses. In conclusion, it seems that a "hormonal imbalance" can have grave consequences not only for the individual itself but also for its descendants.
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics*; D004440:Ecdysone / Q000502:physiology*; D005804:Genes, Lethal / Q000187:drug effects; D005808:Genes, Recessive / Q000187:drug effects; D007605:Juvenile Hormones / Q000502:physiology*; D007814:Larva / Q000502:physiology; D009154:Mutation; D011622:Pterins / Q000494:pharmacology
chemical_list: D007605:Juvenile Hormones; D011622:Pterins; D004440:Ecdysone