Immunological distinction of S-adenosylmethionine synthetase isozymes from rat liver and kidney.
The beta-form of S-adenosylmethionine synthetase among three isozymes has been purified from rat liver, and proven to be homogeneous. The molecular weight of this enzyme was estimated to be 100,000 by gel filtration on Sephacryl S-200, and the enzyme was shown to be composed of two subunits of 48,000 daltons. A rabbit antiserum against the normal rat liver beta-form of S-adenosylmethionine synthetase was used for immunochemical characterization. The alpha- and beta-forms of isozyme are immunochemically identical, but the antiserum did not react with the gamma-form from rat kidney.
D000818:Animals; D000936:Antigen-Antibody Complex; D007106:Immune Sera; D005779:Immunodiffusion; D007527:Isoenzymes / Q000302:isolation & purification*; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008716:Methionine Adenosyltransferase / Q000302:isolation & purification*; D008970:Molecular Weight; D051381:Rats; D014166:Transferases / Q000302:isolation & purification*
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007527:Isoenzymes; D014166:Transferases; D008716:Methionine Adenosyltransferase