Ultrastructural demonstration of immunoglobulin in glutaraldehyde fixed resin embedded human tonsil by an indirect immunoperoxidase method.
A post-embedding method has been established for demonstrating intracellular immunoglobulin using an immunocytochemical technique on material which has been fixed to glutaraldehyde and resin embedded. This allows localisation at both a light and ultrastructural level with good preservation of tissue structure.
D000447:Aldehydes / Q000494:pharmacology*; D005404:Fixatives; D005976:Glutaral / Q000494:pharmacology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins; D014066:Palatine Tonsil / Q000648:ultrastructure*; D010232:Paraffin / Q000494:pharmacology; D012116:Resins, Plant / Q000494:pharmacology*; D014357:Trypsin / Q000494:pharmacology
D000447:Aldehydes; D005404:Fixatives; D007136:Immunoglobulins; D012116:Resins, Plant; D010232:Paraffin; D014357:Trypsin; D005976:Glutaral