[Preparation and application of hybrid antibodies (author's transl)].
Use of hybrid antibodies with one specificity for IgG and the other for an electron microscopical marker is particularly suited for exact localizing of cell antigens. The preparation of hybrid antibodies of anti-marker and anti-IgG immune sera is described. Different ways of this preparation are discussed, whereby a method of production of hybrid antibodies by use of a combined marker- and IgG-immunoadsorbent is suggested. Application of hybrid antibodies with one specificity for IgG and the other for either ferritin or a plant virus (Erysimum latent virus) is demonstrated on visual labeling of cell membrane antigens. The hybrid antibody technique is preferably assumed to allow a multiple labeling of distinct antigens, scanning electron microscopical examinations and quantitative investigations in the field of immuno electron microscopy.
D000818:Animals; D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000032:analysis; D005293:Ferritins; D007118:Immunoassay / Q000379:methods*; D007164:Immunosorbents; D008854:Microscopy, Electron; D010942:Plant Viruses; D012513:Sarcoma, Experimental / Q000276:immunology
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000954:Antigens, Surface; D007164:Immunosorbents; D005293:Ferritins
Präparation und Anwendungsmöglichkeiten von Hybridantikörpern.