Sensitivity and specificity of immunohistological methods on freeze-dried paraffin sections.
The application of freeze-dried paraffin sections for immunohistology eliminates many of the problems associated with the cryostat technique: the sections are thin sufficiently, the cell morphology is improved, there are no diffusion artifacts and the intensity of immunohistological reactions is superior to that obtained on cryostat sections. The preservation of antogenicity in freeze-dried paraffin sections is sufficient for the demonstration of light chain monotypia in lymphomas of B-cell origin. Indirect immunofluorescence is the method of choice for demonstrating surface Ig in freeze-dried paraffin sections.
D000818:Animals; D002999:Clone Cells / Q000276:immunology / Q000473:pathology; D005455:Fluorescent Antibody Technique; D005612:Freeze Drying; D006801:Humans; D007124:Immunoenzyme Techniques; D007147:Immunoglobulin Light Chains / Q000032:analysis; D008223:Lymphoma / Q000032:analysis / Q000276:immunology* / Q000473:pathology; D008867:Microtomy; D014066:Palatine Tonsil / Q000032:analysis / Q000276:immunology / Q000473:pathology; D010232:Paraffin; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell / Q000032:analysis
D007147:Immunoglobulin Light Chains; D011947:Receptors, Antigen, B-Cell; D010232:Paraffin