Helper T-cell-replacing factors and T-cell-growth factors produced by concanavalin A-stimulated and pokeweed mitogen-stimulated mouse spleen cells.
Variables affecting helper factor induction by two T-cell mitogens, concanavalin A (Con A) and pokeweed mitogen (PWM), were examined. It was found that both factors are capable of reconstituting T-cell-dependent antibody response as well as enhancing mitogen-stimulated thymocyte proliferation. Detectable factor production is obtained within 6-12 h of culture; Con A factor production reaches a peak usually around 24 h, while no factor activity is detectable after 72 h of culture period. PWM factor production persists throughout 72 h of culture. Con A factor production can be rescued at culture periods beyond 24 or 48 h by using supraoptimal Con A doses. Con A factor, if incubated at 37 degrees C under cell-free conditions in a serum-free medium, shows a rapid rate of decay; in the presence of cells the decay is considerably reduced.
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000096:biosynthesis / Q000494:pharmacology*; D005260:Female; D006133:Growth Substances / Q000096:biosynthesis*; D007375:Interleukin-1; D007700:Kinetics; D008222:Lymphokines; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011043:Pokeweed Mitogens / Q000494:pharmacology*; D014176:Protein Biosynthesis; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D013696:Temperature
D006133:Growth Substances; D007375:Interleukin-1; D008222:Lymphokines; D011043:Pokeweed Mitogens; C021649:concanavalin A-induced helper factors; D003208:Concanavalin A