Nucleotide sequence of wheat germ cytoplasmic initiator methionine transfer ribonucleic acid.
The primary sequence of wheat germ initiator tRNA has been determined using in vitro labelling techniques. The sequence is: pAUCAGAGUm1Gm2GCGCAG CGGAAGCGUm2GG psi GGGCCCAUt6AACCCACAGm7GDm5Cm5CCAGGA psi CGm1AAACCUG*GCUCUGAUACCAOH. As in other eukaryotic initiator tRNAs, the sequence -T psi CG(A)- present in loop IV of virtually all tRNA active in protein synthesis is absent and is replaced by -A psi CG-. The base pair G2:C71 present in all other initiator tRNAs recognized by E. coli Met-tRNA transformylase is absent and is replaced by U2:A71. Since wheat germ initiator tRNA is not formylated by E. coli Met-tRNA transformylase this implies a possible role of the G2:C71 base pair present in other initiator tRNAs in formylation of initiator tRNA species.
D001483:Base Sequence; D003593:Cytoplasm / Q000502:physiology; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000032:analysis; D010448:Peptide Initiation Factors / Q000235:genetics*; D010944:Plants / Q000378:metabolism*; D014176:Protein Biosynthesis; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics*; D006163:Ribonuclease T1; D012260:Ribonucleases; D014908:Triticum / Q000378:metabolism
D009843:Oligoribonucleotides; D010448:Peptide Initiation Factors; D012346:RNA, Transfer, Amino Acyl; C026464:tRNA(m)(Met), methionine-; D012260:Ribonucleases; D006163:Ribonuclease T1