Immunofluorescent localization of cathepsins B and D in human fibroblasts.
The proteinase cathepsin B has been localized in human fibroblasts using a monospecific antiserum that reacts in a precipitating manner with the denatured enzyme only. The distribution of staining as revealed by both direct and indirect immunofluorescence indicates a typical lysosomal localization similar to that observed for cathepsin D. Using dual localization methods employing fluorescein- and tetramethylrhodamine-labeled affinity purified antibodies both proteinases were usually observed in the same lysosome, indicating a combined role in intracellular digestion.
D002401:Cathepsin B; D002402:Cathepsin D; D002403:Cathepsins / Q000378:metabolism*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D012867:Skin / Q000201:enzymology*
D007106:Immune Sera; D002403:Cathepsins; D002401:Cathepsin B; D002402:Cathepsin D