The importance of the production of phosphatidic acid for the release of arachidonic acid in stimulated platelets.
Phosphatidic acid is formed by platelets as a result of the combined activities of phospholipase C and diacylglycerol kinase. The initial thrombin-activated phospholipase C-mediated reaction is quinacrine insensitive and is followed by quinacrine-sensitive phospholipase A2 activities. The phosphatidic acid released probably results in cellular calcium gating, which, among other actions, might release arachidonic acid by activating phospholipases A2. Indeed, we have detected a phosphatidic acid-specific phospholipase A2 in platelets, which might have an important role in the liberation of arachidonic acid.
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood*; D001792:Blood Platelets / Q000201:enzymology / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D010712:Phosphatidic Acids / Q000097:blood*; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D011796:Quinacrine / Q000494:pharmacology; D013268:Stimulation, Chemical; D013917:Thrombin / Q000494:pharmacology
D001095:Arachidonic Acids; D010712:Phosphatidic Acids; D016718:Arachidonic Acid; D000001:Calcimycin; D010741:Phospholipases A; D054467:Phospholipases A2; D013917:Thrombin; D011796:Quinacrine