Stimulation of prostaglandin cyclooxygenase and prostacyclin synthetase activities by estradiol in rat aortic smooth muscle cells.
The effects of estradiol on the arachidonic acid pool and prostacyclin biosynthetic activity in rat aortic smooth muscle cells were studied. Estradiol has no significant effect on the distribution of [14C]arachidonic acid in cells with respect to prostacyclin production assay, the endogenous fatty acid (specifically, arachidonic acid) composition of cellular phospholipid fractions and cellular phospholipase (or/and lipase) activities. However, estradiol significantly stimulates both prostaglandin cyclooxygenase and prostacyclin synthetase activities of cells, and induction of new protein biosynthesis is involved in the effect of estradiol on the stimulation of prostacyclin biosynthetic activity.
D000818:Animals; D001011:Aorta / Q000201:enzymology; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System; D004789:Enzyme Activation / Q000187:drug effects; D011464:Epoprostenol / Q000096:biosynthesis* / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis; D019746:Intramolecular Oxidoreductases; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D010743:Phospholipids / Q000032:analysis; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D011453:Prostaglandins / Q000096:biosynthesis*; D011463:Prostaglandins H / Q000378:metabolism; D051381:Rats; D013268:Stimulation, Chemical
D001095:Arachidonic Acids; D005227:Fatty Acids; D010743:Phospholipids; D011453:Prostaglandins; D011463:Prostaglandins H; D016718:Arachidonic Acid; D004958:Estradiol; D003577:Cytochrome P-450 Enzyme System; D003513:Cycloheximide; D011464:Epoprostenol; D011451:Prostaglandin-Endoperoxide Synthases; D019746:Intramolecular Oxidoreductases; C022461:prostacyclin synthetase