[Immunohistochemical localization of the associated proteins of high molecular weight (HMWP 350 K, 300 K) in the nervous system of the rat (author's transl)].
High molecular weight microtubule-associated proteins (HMWP) were localized by immunoperoxidase PAP method in Rat nervous system. With tissue sections obtained after alcohol-acid fixation and embedding in paraffin we have observed that: (1) at low antibody concentrations (5 to 10 micrograms/ml) the staining was specific for the central nervous system (CNS) and the peripheral nervous system (PNS); (2) the staining was found in the neurons of the cerebrospinal axis, of the eye, of the paravertebral chains of the sympathetic system, of the plexuses juxta and intra visceral and also in the cells of the adrenal medulla; (3) the dendrites and the pericaryon are the most stained elements of the CNS and PNS respectively; (4) the HMWP are colocalized with tubulin but the reverse is not the case.
D000818:Animals; D001923:Brain Chemistry; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014404:Tubulin / Q000032:analysis
D009419:Nerve Tissue Proteins; D014404:Tubulin
Localisation immunohistochimique dans le système nerveux du rat des protéines de haut poids moléculaire (HMWP 350 K, 300 K) associées à la tubuline]