Factor VIII-related antigen pre-peak on crossed immunoelectrophoresis: a non-random phenomenon.
The incidence of a "pre-peak" over the well on crossed immunoelectrophoresis of factor VIII-related antigen (VIIIR:Ag) was found to be not significantly different in groups of hemophilia carriers and non-carrier women. Only 10% of hemophiliacs more than three days post-transfusion exhibited the feature. Upon transfusion 5 out of 6 hemophiliacs showed a transient appearance of the pre-peak. It was also present during pregnancy in 6 of 8 normal women, 2 hemophilia carriers and two von Willebrand's patients. The pre-peak pattern was quite reproducible both in the same sample tested on different days and in different samples from the same subject. The pre-peak material shows a line of treatment of plasma and by cryoprecipitation.
D000941:Antigens / Q000032:analysis*; D002353:Carrier State / Q000276:immunology; D005169:Factor VIII / Q000008:administration & dosage / Q000032:analysis / Q000276:immunology*; D005260:Female; D006467:Hemophilia A / Q000235:genetics / Q000276:immunology; D006801:Humans; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D007263:Infusions, Parenteral; D011247:Pregnancy; D011897:Random Allocation; D012107:Research Design; D013997:Time Factors; D014842:von Willebrand Diseases / Q000276:immunology
D000941:Antigens; D005169:Factor VIII