[Formation of trypsin inhibitors during alkaline treatment of proteins].
Casein is submitted to a severe alkaline treatment (NaOH 0,2 or 0,5 N, 1 hr., 80 degrees C). The hydrolysis by pancreatic enzymes (trypsin or chymotrypsin) is reduced in vitro and, in the case of the more severe treatment, stopped. After an extended (24 hrs.) trypsin and pronase hydrolysis, it is shown, by affinity chromatography, that peptides, which are not hydrolysable, can bind to trypsin and inactivate this enzyme in vitro.
D002364:Caseins; D002918:Chymotrypsin / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D010446:Peptide Fragments / Q000302:isolation & purification; D011485:Protein Binding; D014357:Trypsin / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000302:isolation & purification
D002364:Caseins; D010446:Peptide Fragments; D014361:Trypsin Inhibitors; D002918:Chymotrypsin; D014357:Trypsin
Mise en évidence de la formation d'inactivateurs de la trypsine au cours de traitements alcalins des protéines.