Hog kidney diamine oxidase conversion of biogenic diamines to inhibitors of cell proliferation.
Hog kidney diamine oxidase (DAO) interacted with 1,3-diaminopropane, putrescine and cadaverine to arrest proliferation of cultured mammalian cells. The byproducts of DAO-substrate interaction, H2O2 and NH4+ are themselves cytotoxic but were apparently not responsible for any significant antiproliferative effect in the experimental system. DAO is known to react with putrescine to generate labile 4-aminobutyraldehyde. This primary product was compatible with a compound (radio-labelled) separated from DAO-putrescine reactive mixtures by ion-exchange chromatography. Its rate of generation, level attained and lability (half-life, 2 hr) in culture simulated conditions were measured. Circumstantial evidence suggested that the amine aldehyde products of diamine oxidation had a potent antiproliferative effect on cells.
D000447:Aldehydes / Q000378:metabolism; D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism*; D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003959:Diamines / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D008214:Lymphocytes / Q000187:drug effects; D010084:Oxidation-Reduction; D011700:Putrescine / Q000378:metabolism; D013552:Swine
D000447:Aldehydes; D001679:Biogenic Amines; D003959:Diamines; C006509:4-aminobutyraldehyde; D006631:Amine Oxidase (Copper-Containing); D011700:Putrescine