Structural analysis of adult and larval isozymes of sn-glycerol-3-phosphate dehydrogenase of Drosophila melanogaster.
Compositional analysis of the soluble tryptic peptides representing about 70% of the 293 residues of sn-glycerol-3-phosphate dehydrogenase in Drosophila melanogaster reveals a single peptide difference between the sn-glycerol-3-phosphate dehydrogenase adult (GPDHF-1) and larval (GPDHF-3) isozymes. This peptide was shown to be the carboxyl terminus by sequence determination and by carboxypeptidase A digestion of the native protein. For GPDHF-1, the sequence of the COOH-terminal tryptic peptide is Asn-His-Pro-Glu-His-Met-Gln-Asn-Leu-COOH, while that of GPDHF-3 is Asn-His-Pro-Glu-His-Met-COOH.
D000595:Amino Acid Sequence; D000818:Animals; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; D004331:Drosophila melanogaster / Q000201:enzymology*; D010450:Endopeptidases; D005993:Glycerolphosphate Dehydrogenase; D007527:Isoenzymes; D007814:Larva / Q000201:enzymology; D010446:Peptide Fragments / Q000032:analysis; D012697:Serine Endopeptidases; D014357:Trypsin
D007527:Isoenzymes; D010446:Peptide Fragments; D005993:Glycerolphosphate Dehydrogenase; D002268:Carboxypeptidases; D010450:Endopeptidases; D043422:Carboxypeptidases A; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D014357:Trypsin