[Preservation of the allosteric properties of the protein receptor for acetylcholine in detergent solution without addition of lipids].
Solubilization by Na cholate of acetylcholine receptor-rich membrane fragments from Torpedo marmorata, followed by exchange of NA cholate by the neutral detergent "Tween 80" yields the receptor protein in its 9 S soluble light form; under these conditions, without adding lipids, the receptor protein conserves its characteristic binding properties for the fluorescent agonist Dns-C6-Cho as followed by fast kinetic techniques, and the allosteric regulation by non-competitive blockers.
D000494:Allosteric Regulation; D003619:Dansyl Compounds / Q000378:metabolism; D007700:Kinetics; D008722:Methods; D011136:Polysorbates; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000378:metabolism*
D003619:Dansyl Compounds; D011136:Polysorbates; D000644:Quaternary Ammonium Compounds; D011950:Receptors, Cholinergic; C027933:(1-(5-dimethylaminonaphthalene)sulfonamido)-n-hexanoic acid-beta-N-trimethylammonium ethyl ester
Conservation des propriétés allostériques de la protéine réceptrice de l'acétylcholine en solution détergente sans addition de lipides.