title: Interaction of rat platelets with Listeria monocytogenes.
abstract: Listeria monocytogenes induced aggregation of rat platelets in vitro and stimulated the nonlytic release of [3H]serotonin. Listeria-induced platelet aggregation and serotonin release required the presence of intact Listeria, was maximal at a 1:1 Listeria/platelet ratio, required a plasma cofactor, and was not inhibited by indomethacin, acetylsalicylic acid, or apyrase. Aggregation either of platelets in platelet-rich plasma with adenosine diphosphate or of washed platelets with thrombin resulted in the release of a listericidin from the platelets; however, direct interaction of L. monocytogenes with platelet-rich plasma did not kill Listeria. The ability of rats to clear an intravenous challenge of L. monocytogenes (0.005 50% lethal dose), as determined by the recovery of viable L. monocytogenes from the spleen and liver, was unaffected by prior treatment with antiplatelet serum.
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000502:physiology*; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D008089:Listeria monocytogenes / Q000502:physiology*; D008088:Listeriosis / Q000276:immunology; D010974:Platelet Aggregation; D051381:Rats; D012701:Serotonin / Q000378:metabolism*
chemical_list: D012701:Serotonin; D000244:Adenosine Diphosphate; D001241:Aspirin; D007213:Indomethacin