A rapid procedure for the isolation of human IgM myeloma proteins.
A two-step rapid procedure for the isolation of human IgM paraproteins from plasma samples is described. It involves gel filtration on Ultrogel AcA 34 followed by anion exchange chromatography on DEAE-Sepharose CL6B. The overall yield of IgM is greater than 70% and the final product is pure as judged by immunoelectrophoresis and SDS-polyacrylamide gel electrophoresis. The procedure is applicable to both euglobulin and cryoglobulin IgM proteins of a wide range of electrophoretic mobilities.
D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007075:Immunoglobulin M; D007158:Immunologic Techniques; D008722:Methods; D009194:Myeloma Proteins / Q000302:isolation & purification*; D008258:Waldenstrom Macroglobulinemia / Q000276:immunology
D007075:Immunoglobulin M; D009194:Myeloma Proteins