The isolation of human pituitary hormones from frozen glands.
An integrated scheme for the extraction and purification of human pituitary hormones from frozen glands has been developed. The method yields 6.3 mg/gland of monomeric pyrogen-free GH in a single fraction, with a potency of 2.5-3.1 IU/mg, as estimated by bioassay, radioreceptor assay, and RIA, with a further 0.6 mg/gland recovered from side fractions. A glycoprotein fraction yields 200 IU FSH, 500 IU LH, and 0.2 IU TSH per gland, as estimated by RIA. Additional hormones purified from side fractions include PRL, lipotropins, and the 20,000 molecular weight variant of human GH.
D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D005640:Follicle Stimulating Hormone / Q000302:isolation & purification; D005615:Freezing; D013006:Growth Hormone / Q000302:isolation & purification; D006801:Humans; D007525:Isoelectric Focusing; D007986:Luteinizing Hormone / Q000302:isolation & purification; D010902:Pituitary Gland / Q000032:analysis*; D010907:Pituitary Hormones / Q000302:isolation & purification*; D011388:Prolactin / Q000302:isolation & purification; D013048:Specimen Handling; D008083:beta-Lipotropin / Q000302:isolation & purification
D010907:Pituitary Hormones; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013006:Growth Hormone; D008083:beta-Lipotropin