Immunochemical quantitation of human submandibular-sublingual lysozyme.
A method for quantitating lysozyme by using carbamylated antiserum (commercially available) to human lysozyme in a electroimmunodiffusion technique has been developed. The method measures specific protein not lytic activity as the lyso-plate method does. We have applied this method to tears, parotid saliva, submandibular-sublingual saliva, nasal secretions, serum, and minor salivary gland secretions. We specifically selected submandibular-sublingual saliva for a comparison test with the lyso-plate method because of the known mucin content of the submandibular-sublingual saliva. Our findings indicate that no pretreatment is necessary for the electroimmunodiffusion technique. The lyso-plate method, on the other hand, requires pretreatment with Tris-acetate pH 4.5 to dissociate the mucin-lysozyme complex and give accurate values.
D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D005779:Immunodiffusion; D009113:Muramidase / Q000276:immunology* / Q000652:urine; D009297:Nasal Mucosa / Q000378:metabolism; D012463:Saliva / Q000276:immunology; D013361:Sublingual Gland / Q000201:enzymology*; D013363:Submandibular Gland / Q000201:enzymology*; D013666:Tears / Q000276:immunology; D013696:Temperature
D007106:Immune Sera; D009113:Muramidase