T cell subpopulations required for the human cytotoxic T lymphocyte response to influenza virus: evidence for T cell help.
The requirement for specific T cell subsets in the in vitro generation of human influenza virus-immune cytotoxic T lymphocyte (CTL) responses was investigated. Peripheral blood T cell populations were identified by and selected for their reactivity with the monoclonal antibodies OKT3, OKT4, and OKT8. Influenza virus-immune CTL effectors are OKT3+, OKT4-, and OKT8+. Analysis of CTL precursors demonstrates that: 1) influenza-immune CTL precursors are OKT3+, OKT4-, and OKT8+; 2) CTL precursors require radioresistant OKT3+ helper T cells in order to generate strong cytotoxic responses; and 3) helper T cells can be obtained from either the OKT4+ or OKT4- populations, but more potent help was generated by the OKT4+ subset. The demonstration of a helper T cell requirement for the generation of influenza virus-immune CTL suggests that helper T cells may be involved in the previously documented HLA-linked Ir gene control of influenza-immune CTL responsiveness.
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D003602:Cytotoxicity, Immunologic; D006041:Goats; D006801:Humans; D007251:Influenza, Human / Q000276:immunology*; D008297:Male; D051379:Mice; D013601:T-Lymphocytes / Q000145:classification*
D000906:Antibodies; D000911:Antibodies, Monoclonal