Genetic analysis of immune suppression. I. Gene complementation is required for suppression of antigen-specific proliferative responses by T-cell derived factors.
We have analyzed the genetic control of susceptibility to suppression by 1-J+ , suppressor-T-cell derived factors (TsF) specific for the synthetic polymer L-glutamic acid50-L-tyrosine50 (GT). GT-TsF activity was measured as specific inhibition of proliferative responses to GT developed in cultures of lymph-node T cells from mice primed with GT complexed to methylated bovine serum albumin (GT-MBSA). These experiments demonstrated that there is no MHC-encoded genetic restriction between donors and recipients of GT-TsF in suppression of proliferative responses. We have also confirmed the observations that mice of the H-2b, H-2d, and H-2k haplotypes can produce GT-TsF, whereas H-2a mice do not, and that H-2a, H-2d, and H-2k mice are sensitive to GT-TsF from all producer strains, whereas H-2b mice are not sensitive to GT-TsF from any strain. Analysis of the effect of GT-TsF on responses by mice bearing recombinant haplotypes suggests that at least two genes are required for susceptibility to GT-TsF and that these genes show coupled complementation.
D000818:Animals; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000235:genetics; D007111:Immunity, Cellular; D007165:Immunosuppression Therapy; D036341:Intercellular Signaling Peptides and Proteins; D008213:Lymphocyte Activation; D051379:Mice; D010455:Peptides / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D006183:H-2 Antigens; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C027606:L-glutamic acid-L-tyrosine copolymer