Characterization of porcine adrenocortical lysosomes.
Porcine adrenocortical lysosomes were characterized by differential centrifugation, acid hydrolase contents, latency of cathepsin D, release of bound acid hydrolases in soluble form, and isopycnic density gradient centrifugation. Cathepsins D and B, beta-N-acetylglucosaminidase, beta-galactosidase and arylsulphatase were found exclusively in the lysosomes, while alpha-mannosidase and beta-glucuronidase were in both the lysosomal and microsomal fractions. The activity of cathepsin D was remarkably high, amounting to more than 6 times that in porcine liver and to more than 10 times that in liver of Sprague-Dawley rats in terms of units per g wet tissue. Porcine adrenocortical lysosomes showed a modal isopycnic density value of 1.155, but mitochondria a value of 1.145. The validity of these values was studied by investigating the possibilities of agglutination of organelles, damage to lysosomal membranes, disruption of mitochondria due to the hydrostatic pressure and by applying the same procedures of isopycnic centrifugation to hog and rat livers. After these validity tests, porcine adrenocortical lysosomes were concluded to be unique in their strikingly high content of cathepsin D as well as in their low modal isopycnic density which is very close to that of porcine adrenocortical mitochondria.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000302:Adrenal Cortex / Q000201:enzymology*; D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism; D002401:Cathepsin B; D002402:Cathepsin D; D002403:Cathepsins / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D005966:Glucuronidase / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D008361:Mannosidases / Q000378:metabolism; D051381:Rats; D013552:Swine / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
D001192:Arylsulfatases; D008361:Mannosidases; D001616:beta-Galactosidase; D005966:Glucuronidase; D000118:Acetylglucosaminidase; D002403:Cathepsins; D002401:Cathepsin B; D002402:Cathepsin D