Development of a simple passive haemagglutination-inhibition assay for Listeria monocytogenes lipoteichoic acid.
A simple assay for listeria lipoteichoic acid was devised. It is based on the property of this substance to attach spontaneously to erythrocytes. Thus coated rabbit erythrocytes were agglutinated by a rabbit antiserum raised towards dead listeria. Inhibition of this passive agglutination was used as a measure of listeria lipoteichoic acid. Cross-reactivity of lipoteichoic acid from Lactobacillus fermenti in the assay and inhibition by simple sugars suggest that the assay is specific for terminal galactose.
D000818:Animals; D004912:Erythrocytes; D005690:Galactose / Q000494:pharmacology; D006385:Hemagglutination Inhibition Tests / Q000379:methods; D006386:Hemagglutination Tests; D007106:Immune Sera / Q000494:pharmacology; D007778:Lactobacillus / Q000276:immunology; D008070:Lipopolysaccharides; D008089:Listeria monocytogenes / Q000276:immunology*; D008297:Male; D051379:Mice; D010712:Phosphatidic Acids / Q000032:analysis*; D011817:Rabbits; D012756:Sheep; D013211:Staphylococcus aureus / Q000276:immunology; D013682:Teichoic Acids / Q000032:analysis*
D007106:Immune Sera; D008070:Lipopolysaccharides; D010712:Phosphatidic Acids; D013682:Teichoic Acids; C009900:lipoteichoic acid; D005690:Galactose