[Phalloidin counteracts the destruction of F-actin by osmic acid. II. Protection by phalloidin of F-actin crosslinked with aldehydes (author's transl)].
We have studied by viscometry and spectrophotometry the effects of glutaraldehyde, acetaldehyde, acrolein and formaldehyde on F-actin in vitro. Pretreatment with acrolein and, to a lesser extent, with glutaraldehyde, results in increased destruction of F-actin during the subsequent degradation with osmic acid. Formaldehyde alone disintegrates actin filaments. Acetaldehyde gives the best results and does not seem to damage F-actin. Phalloidin protects F-actin against destruction by osmic acid and this protection is also observed in the F-actin which has been pretreated with the four aldehydes mentioned.
D000079:Acetaldehyde / Q000494:pharmacology; D000171:Acrolein / Q000494:pharmacology; D000199:Actins / Q000378:metabolism*; D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D005557:Formaldehyde / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D009842:Oligopeptides / Q000494:pharmacology*; D009992:Osmium / Q000494:pharmacology*; D009993:Osmium Tetroxide / Q000494:pharmacology*; D010590:Phalloidine / Q000494:pharmacology*; D011817:Rabbits
D000199:Actins; D000447:Aldehydes; D009842:Oligopeptides; D010590:Phalloidine; D005557:Formaldehyde; D009992:Osmium; D000171:Acrolein; D000079:Acetaldehyde; D009993:Osmium Tetroxide; D005976:Glutaral
La phalloïdine protège la F-actine contre les effets destructeurs de l'acide osmique. II. Protection par la phalloïdine de la F-actine traitée aux aldéhydes.