The relationship between metabolism of 2,3,4,5,3',4',5'-heptachlorobiphenyl and its ability to induce both cytochromes P-448 and P-450.
Male CD rats received i.p. injections of 2,3,4,5,3',4',5'-heptachlorobiphenyl (7CB) under conditions resulting in induction of both benzphetamine demethylase (cytochrome P-450-dependent) and ethoxyresorufin deethylase (cytochrome P-448-dependent) activities. To test the possibility that one class of induction (P-450) was due to the parent compound and the other (P-448) to a metabolite, liver, adipose, bile, urine and feces were analyzed for the presence of 3,4,5,3',4',5'-hexachlorobiphenyl (6CB) (the major photolysis product of 7CB) and/or 3-hydroxy-3,4,5,3',4',5'-hexachlorobiphenyl (OH-6CB), another known P-448 inducer. To allow for the possibility that 6CB might be a transient intermediate. The results supported the hypothesis that 7CB is a true 'mixed inducer', nor requiring metabolic alteration for its dual activity.
D000273:Adipose Tissue / Q000201:enzymology; D000818:Animals; D019388:Cytochrome P-450 CYP1A2; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D003580:Cytochromes / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000187:drug effects; D005243:Feces / Q000032:analysis; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D003580:Cytochromes; C034559:2,3,4,5,3',4',5'-heptachlorobiphenyl; D003577:Cytochrome P-450 Enzyme System; D011078:Polychlorinated Biphenyls; D006899:Mixed Function Oxygenases; C017170:Cyp1a2 protein, rat; D019388:Cytochrome P-450 CYP1A2