title: [Lysine biosynthesis of Pseudomonas aeruginosa PAO1. III. Further characterization of lysine auxotrophic mutant of Ps. aeruginosa PAO1].
abstract: A number of lysine-auxotrophic mutants of Pseudomonas aeruginosa PAO1 were isolated through mutagenesis by means of N-methyl-N-nitrosoguaniine (Mach et al., unpublished). Using the cross feeding test and growth tests classification of lysine mutants was not possible. The investigation of diaminopimelic acid decarboxylase (DAP-DC) showed, that none of these mutants had an active enzyme, except for the mutants with a high number of revertants. The appearance of only one mutant type is attributed to the insufficient availability of DAP.
mesh_terms: D001426:Bacterial Proteins; D002262:Carboxy-Lyases / Q000378:metabolism; D008239:Lysine / Q000235:genetics*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008769:Methylnitronitrosoguanidine; D002262:Carboxy-Lyases; C079912:LysA protein, Bacteria; C020031:diaminopimelic acid decarboxylase; D008239:Lysine
vernacular_title: Lysinbiosynthese bei Pseudomonas aeruginosa PAO1. III. Weitere Charakterisierung Lysin-auxotropher Mutanten von Ps. Aeruginosa PAO1.