[Survey of "Neisseria gonorrhoeae" sensitivity to antibiotics by dilution and diffusion methods using a new medium (author's transl)].
Usual media are not convenient for the determination of minimal inhibitory concentrations (MIC). An agar medium without haemoglobin and seric proteins (ABNG) is proposed. Using the dilution method with less fastidious bacteria, including Neisseria sp., MIC were identical on Mueller-Hinton agar and ABNG. By using diffusion techniques, the correlation was good between MIC and zone diameters; the density of inoculum for gonococci was 2 x 10(6) CFU/ml. Dilution and diffusion techniques were used for penicillin, ampicillin, thiamphenicol, tetracycline and spectinomycin. It appears that the diffusion technique with ABNG medium would simplify the antibiotic sensitivity survey for N. gonorrhoeae, dilution technique remaining the reference method.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003470:Culture Media; D004058:Diffusion; D008826:Microbial Sensitivity Tests / Q000379:methods; D009344:Neisseria gonorrhoeae / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D003470:Culture Media
Détermination de la sensibilité de Neisseria gonorrhoeae aux antibiotiques par dilution et diffusion en gélose à l'aide d'un nouveau milieu.