[Production of antibodies to a specific estrogen-binding protein of rat liver and study of the reaction between isolated immunoprecipitates and estradiol].
High-selective antiserum (AS) against unusual estrogen-binding protein (UEBP) from male rat liver was obtained according to the following technique: 1) immunization of the rabbits by partially purified UEBP and isolation of polyspecific AS; 2) depletion of the latter AS by the UEBP-free fraction of female rat liver proteins prepared under the conditions similar to those employed in the procedure for isolation of the UEBP preparation. High specificity of depleted AS for UEBP was demonstrated by immunodiffusion, immunoelectrophoresis and agar-gel electrophoresis of UEBP and by the detected specific estradiol (E2)-binding capacity of immunoprecipitates obtained after the reaction of depleted AS with partially purified UEBP. Analysis of E2-binding activity of immunoprecipitates may be used for the discovery of UEBP antibodies in the polyspecific AS.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000918:Antibody Specificity; D002352:Carrier Proteins / Q000276:immunology*; D004587:Electrophoresis, Agar Gel; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008099:Liver / Q000276:immunology*; D008297:Male; D011485:Protein Binding; D011817:Rabbits / Q000276:immunology; D051381:Rats; D011960:Receptors, Estrogen
D000906:Antibodies; D002352:Carrier Proteins; D011960:Receptors, Estrogen; C010140:estrophilin; D004958:Estradiol
Poluchenie antitel k osobomu éstrogensviazyvaiushchemu belku pecheni krys i izuchenie vzaimodeĭstviia vydelennykh immunopretsipitatov s éstradiolom.