[Determination of glucoamylase activity using an enzyme electrode].
Both, the differing definitions of glucoamylase activity and the different determination of the fragment, glucose, preclude a comparison of literature data. Specific enzymatic determination of the fragment, glucose, with an enzyme electrode is proposed. The contribution describes the manufacture of an enzyme electrode from a commercial pO2-electrode and immobilized GOD. The measurement may be carried out by kinetic determination of utilized oxygen or oxygen peroxide formed. The continuous measurement proposed for serial experiments is made via a calibration curve, whose regression coefficient was determined to be 0.999. Depending on the enzyme activity, the reaction time is between 15 s and 3 min.
D004566:Electrodes; D004800:Enzymes, Immobilized; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism*; D005949:Glucose Oxidase; D005959:Glucosidases / Q000378:metabolism*; D007700:Kinetics; D008722:Methods; D004718:Saccharomycetales / Q000201:enzymology
D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D005959:Glucosidases; D005087:Glucan 1,4-alpha-Glucosidase
Bestimmung der Glucoamylase-Aktivität mit Hilfe einer Enzymelektrode.