T cell-mediated cytotoxicity induced by Listeria monocytogenes. III. Phenotypic characteristics of mediator T cells.
Cytotoxic thymus-derived lymphocytes (CTL) generated in vitro by restimulating rat cells with Listeria antigen- (LMA) pulsed syngeneic accessory cells were characterized in respect to their surface membrane markers. LM-dependent CTL were devoid of detectable surface immunoglobulin (Ig) and receptors for the Fc region of rabbit IgG. Experiments with monoclonal antibodies to rat T cell markers revealed that these cytotoxic cells have the phenotypic profile W3/13+, W3/25-, MRC OX 8+. LM-dependent CTL also bind the monoclonal antibody, MRC OX 3, which recognizes an Ia-antigen-like determinant on rat cells. Although LM-dependent CTL lack the W3/25 marker, their generation depends on the cooperative interplay of W3/25+ and W3/25- T cells.
D000818:Animals; D002417:Cattle; D005591:Chemical Fractionation; D003602:Cytotoxicity, Immunologic; D008089:Listeria monocytogenes / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D010641:Phenotype; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D011961:Receptors, Fc; D013601:T-Lymphocytes / Q000276:immunology*
D011961:Receptors, Fc