Attachment of Mycoplasma hominis and M. orale to human diploid lung fibroblasts.
The process of attachment of Mycoplasma hominis and M. orale to HAIN-55 cells, derived from normal embryonic human lung, was investigated quantitatively. The attachment reached its maximum within about 2-4 hr at 37 degrees C and increased linearly as a function of the number of organisms present in the system. The relative attachment efficiency of M. hominis was approximately 1% under our experimental conditions. Trypsin and EDTA were effective in detaching particles of M. hominis and M. orale from the surfaces of HAIN-55 cells. Therefore it was suggested that some proteinaceous substance and salt bridges might be involved in the attachment of these mycoplasmas to HAIN-55 cells.
D000268:Adhesiveness; D002455:Cell Division; D002460:Cell Line; D004492:Edetic Acid / Q000494:pharmacology; D005347:Fibroblasts / Q000382:microbiology*; D006801:Humans; D007700:Kinetics; D008168:Lung; D009174:Mycoplasma / Q000502:physiology*; D014357:Trypsin / Q000494:pharmacology
D004492:Edetic Acid; D014357:Trypsin