title: An autoanalyzer test to determine immunoglobulin class and IgG subclass of blood group antibodies.
abstract: An automated antiglobulin test was used to characterize the immunoglobulin class and IgG subclass of red blood cell-bound antibodies. Immunoglobulin classes and IgG subclasses of some clinically significant antibodies (anti-D and anti-CD, anti-Fy, anti-Jka) as well as of clinically insignificant anti-Chido antibodies were determined. Eighteen anti-Duffy antibodies were structurally homogeneous, and were mostly composed only of IgG1. Of the 16 anti-Fya antibodies studied only three had IgG2, and four had IgM molecules. Ten anti-Jka antibodies were heterogeneous, but all samples were either IgG1 or IgG3, or both. The most prominent immunoglobulin of 12 hyperimmune anti-Rh (anti-D and anti-CD) antibodies was IgG1. Under the conditions of our test, we also detected IgG3 and very low concentrations of IgG2 and IgG4 molecules in the anti-Rh antibodies.
mesh_terms: D001322:Autoanalysis; D001789:Blood Group Antigens / Q000276:immunology*; D003298:Coombs Test; D004375:Duffy Blood-Group System / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A; D007074:Immunoglobulin G / Q000145:classification; D007075:Immunoglobulin M; D007136:Immunoglobulins / Q000145:classification*; D007667:Kidd Blood-Group System / Q000276:immunology; D012204:Rh-Hr Blood-Group System / Q000276:immunology
chemical_list: D001789:Blood Group Antigens; D004375:Duffy Blood-Group System; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D007667:Kidd Blood-Group System; D012204:Rh-Hr Blood-Group System