title: A hormone dependent calcium-binding protein in the mantle edge of the freshwater snail Lymnaea stagnalis.
abstract: By means of gel filtration and ion exchange chromatography on calcium-saturated Chelex-100, a calcium-binding fraction was isolated from the mantle edge of the freshwater snail lymnaea stagnalis. This fraction was not present in other tissues. Treatment with trypsin caused a disappearance of the calcium-binding capacity, proving that the active substance in this fraction is a protein (calcium-binding protein; CaBP). Removal of the growth hormone-producing neuroendocrine light green cells resulted in a  strong decrease of the amount of CaBP. It is concluded that L. stagnalis possesses a hormone-dependent CaBP, probably responsible for the maintenance of a high calcium concentration in that part of the mantle that produces the outer crystalline layer of the shell.
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008195:Lymnaea / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology
chemical_list: D002135:Calcium-Binding Proteins; D014357:Trypsin; D002118:Calcium