Diacylglycerol metabolism and arachidonic acid release in human fetal membranes and decidua vera.
Diacylglycerol lipase activity has been demonstrated in human fetal membranes and decidua vera tissues. The specific activity of the enzyme is highest in the microsomal fraction of decidua vera tissue. The acylester bond at the sn-1 position of 1,2-diacyl-sn-glycerol is hydrolyzed followed by release of the fatty acid at the sn-2 position. The diacylglycerol lipase activity present in the microsomal fraction of decidua vera tissue hydrolyzes preferentially a diacylglycerol containing an arachidonoyl group in the sn-2 position. Monoacylglycerol lipase activity was also demonstrated in these tissues. The specific activity of monoacylglycerol lipase was significantly greater than that of diacylglycerol lipase and catalyzed preferentially the hydrolysis of monoacylglycerols containing an arachidonyl group in the sn-2 position. Based on the subcellular distribution and the differential effects of various inhibitors, we suggest that the monoacylglycerol lipase and diacylglycerol lipase in decidua vera tissue are 2 distinct enzymes. Diacylglycerol kinase specific activity was examined also and was found to be 4-5 times greater in amnion than in either chorion laeve or decidua vera. The importance of diacylglycerol metabolism in the mechanism of arachidonic acid release and prostaglandin biosynthesis is discussed.
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002250:Carbon Radioisotopes; D003656:Decidua / Q000201:enzymology*; D004075:Diglycerides / Q000378:metabolism*; D005321:Extraembryonic Membranes / Q000201:enzymology*; D005260:Female; D005989:Glycerides / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D007700:Kinetics; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008861:Microsomes / Q000201:enzymology; D009829:Oleic Acids / Q000378:metabolism; D011247:Pregnancy; D013329:Structure-Activity Relationship; D014316:Tritium
D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D004075:Diglycerides; D005989:Glycerides; D009829:Oleic Acids; D014316:Tritium; D016718:Arachidonic Acid; D008071:Lipoprotein Lipase