title: Microprocedure for determination of carbaryl in blood and tissues.
abstract: A microprocedure was adapted for the extraction and cleanup of carbaryl from blood and tissues of animals poisoned with this insecticide. Ball-mill extraction using acetone and methylene chloride, removal of lipid material by a freeze-out procedure, and florisil microcolumn cleanup were employed. Derivatization of carbaryl with heptafluorobutyric anhydride in the presence of trimethylamine allowed rapid processing for gas-liquid chromatographic separation on 3% OV-17 and electron-capture detection. Limits of carbaryl detection were 0.02 ppm for blood and 0.1 ppm for tissue. This procedure allowed simultaneous processing and sensitive detection of carbaryl in numerous small samples.
mesh_terms: D000818:Animals; D012721:Carbaryl / Q000032:analysis* / Q000506:poisoning; D002849:Chromatography, Gas / Q000379:methods; D008832:Microchemistry; D051381:Rats; D012756:Sheep; D012757:Sheep Diseases / Q000139:chemically induced / Q000378:metabolism
chemical_list: D012721:Carbaryl