3H-collagen turnover in non-cross-linked and aldehyde-cross-linked dermal collagen grafts.
Using trypsin-purified rat dermal collagen labelled with tritiated hydroxyproline and proline, a study has been made of hydroxyproline turnover in non-cross-linked and glutaraldehyde- and formaldehyde-cross-linked collagen when implanted s.c. in unlabelled isogenic rats. Grafts cross-linked with 0.01% glutaraldehyde maintained their collagen mass over a 22-week period, loss of original collagen being balanced by the gain in new collagen (22% at 22 weeks). Cross-linking with 5% formaldehyde temporarily inhibited collagen loss as compared with non-cross-linked grafts. However, at 22 weeks both had lost some 30% of their collagen mass, the gain of new collagen (some 8%) only partially compensating for the loss of original implant collagen.
D000818:Animals; D003094:Collagen / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D005557:Formaldehyde / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D011392:Proline / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D016038:Skin Transplantation
D003432:Cross-Linking Reagents; D005557:Formaldehyde; D003094:Collagen; D011392:Proline; D006909:Hydroxyproline; D005976:Glutaral