title: An observation chamber for a high-pressure stopped-flow apparatus.
abstract: A new design of the observation window to observation chamber seal is described for the Berger rapid stopped-flow apparatus. This design permits reliable operation of the apparatus and retains the features of a square non-deviating observation path with flush windows. Incorporation of fiber optic light guides for the observation path has made the apparatus insensitive to vibration artifacts. A demonstration reaction of calcium binding to EGTA is presented; the reaction, which is 90% complete within 2 ms, illustrates the capability of this apparatus for examining fast reactions.
mesh_terms: D001671:Biochemistry / Q000295:instrumentation*; D002118:Calcium / Q000378:metabolism; D004533:Egtazic Acid / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D011312:Pressure
chemical_list: D004533:Egtazic Acid; D002118:Calcium