Inhibitory effects of alkylated Bence-Jones protein, k-type light chain, from urine of a patient with macroglobulinemia on gastric juice secretion and ulceration in rats.
Bence-Jones protein (BJP) was isolate from the urine of a patient with macroglobulinemia by salting out the ammonium sulfate, followed by chromatography on DEAE-cellulose column and gel filtration. This native BJP was identified as k-type light chain by immunoelectrophoresis and the native light chain showed almost no activity of inhibiting the gastric ulceration in pylorus-ligated rats. In contrast, reduction of the disulfide bond by 2-mercaptoethanol and alkylation of the thiol by iodoacetate in this native light chain resulted in significant inhibition of the gastric ulceration. The alkylated light chain also showed a significant inhibition of gastric juice secretion in pylorus-ligated rats.
D000478:Alkylation; D000818:Animals; D000897:Anti-Ulcer Agents; D001285:Atropine / Q000494:pharmacology; D001536:Bence Jones Protein / Q000652:urine*; D005750:Gastric Juice / Q000378:metabolism*; D006801:Humans; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D008258:Waldenstrom Macroglobulinemia / Q000652:urine*
D000897:Anti-Ulcer Agents; D001285:Atropine; D001536:Bence Jones Protein