Effect of procarbazine treatment of mice on alpha-glycerolphosphate dehydrogenase activity and frequency of selected abnormalities in sperm.
The in situ activity of mitochondrial alpha-glycerol phosphate dehydrogenase (alpha-GPD) as well as 2 specific sperm abnormalities [headless and disorganization of the mitochondria assembly (PL-type)] have been studied in sperm from mice treated with single doses of procarbazine (100, 200, 400, 600 and 800 mg/kg) at intervals up to 55 days after treatment. The frequency of sperm without active alpha-GPD and the frequency of the morphology variants increased with increasing dose of procarbazine. The variance in the level of alpha-GPD among sperm with active enzyme and the variance between animals also increased with increasing dose. The lowest effective dose was 200 mg/kg and there were no observable effects 85 days after treatment.
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005787:Gene Frequency; D014644:Genetic Variation; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA / Q000235:genetics; D008854:Microscopy, Electron; D009153:Mutagens; D011344:Procarbazine / Q000494:pharmacology*; D013094:Spermatozoa / Q000201:enzymology / Q000648:ultrastructure*
D009153:Mutagens; D011344:Procarbazine; D005993:Glycerolphosphate Dehydrogenase