I-A antigens on cloned alloreactive murine T lymphocytes are acquired passively.
Cloned lines of alloreactive cytolytic and amplifier T lymphocytes have been examined for expression of I-A antigens. Using flow cytofluorometry and monoclonal antibodies, MHC antigens representing both I-A and H-2K or D determinants were reproducibly detected on the cell surface of these cloned T lymphocytes. All cloned lines examined expressed H-2K or D antigens representing the MHC haplotype appropriate to their strain of origin. However, I-A as well as H-2K or D antigens appeared to be acquired passively from the stimulating cell population. Within the lower limits of detection using the fluorescence-activated cell sorter, evidence suggesting endogenous synthesis of I-A antigens by these cloned T lymphocytes could not be demonstrated. Further, the observed binding appeared to be nonspecific, because material originating from allogeneic, syngeneic, or third-party stimulating cells could be observed under appropriate conditions.
D000818:Animals; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005455:Fluorescent Antibody Technique; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D007112:Immunity, Maternally-Acquired; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II