Requirement of a protein component for scrapie infectivity.
Scrapie infectivity in the Hirt supernatant of a 20% scrapie-infected mouse brain filtrate was almost totally inactivated by pronase treatment. It was not affected by RNase A treatment and was only slightly reduced by DNase I treatment. These results indicate that a protein component is required for the transfection of scrapie nucleic acid and that free scrapie nucleic acid is either not infectious or its infectivity is extremely low.
D000818:Animals; D004279:DNA, Viral / Q000235:genetics; D010447:Peptide Hydrolases; D011328:Prions / Q000032:analysis*; D012608:Scrapie / Q000382:microbiology*; D012756:Sheep; D014764:Viral Proteins / Q000502:physiology*
D004279:DNA, Viral; D011328:Prions; D014764:Viral Proteins; D010447:Peptide Hydrolases