Factor VIII/von Willebrand factor protein: sensitivity of periodic acid Schiff stain to carbohydrate deficiency.
We have investigated the periodic acid Schiff (PAS) Coomassie staining ratio of the human factor VIII/von Willebrand factor (fVIII/vWf) protein. The PAS-Coomassie staining ratio is consistent over 8 days. The PAS-Coomassie ratio of fVIII/vWf protein purified from different starting materials does not appear to be significantly different. The PAS stain can detect as little as 300 ng of carbohydrate in the fVIII/vWf protein. Desialation did not affect the PAS-Coomassie ratio, while removal of penultimate galactose resulted in a marked reduction in the PAS-Coomassie ratio. This reduction was further accentuated with the removal of N-acetylglucosamine. The smaller multimers of the fVIII/vWf protein have a reduced sialic acid and PAS-Coomassie staining ratio. This difference does not appear to be related to the sialic acid deficiency but may be related to the distribution or organization of the carbohydrate moieties on the smaller fVIII/vWf multimers.
D001779:Blood Coagulation Factors / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D005169:Factor VIII / Q000032:analysis*; D006651:Histocytochemistry; D006801:Humans; D010503:Periodic Acid-Schiff Reaction; D012394:Rosaniline Dyes; D012794:Sialic Acids / Q000032:analysis; D014841:von Willebrand Factor / Q000032:analysis*
D001779:Blood Coagulation Factors; D002241:Carbohydrates; D012394:Rosaniline Dyes; D012794:Sialic Acids; D014841:von Willebrand Factor; D005169:Factor VIII; C004692:coomassie Brilliant Blue