Enhanced susceptibility of glutaraldehyde-treated tumor cells to antibody-dependent macrophage-mediated cytolysis.
The effect of treatment of target tumor cells with glutaraldehyde (GA) on antibody-dependent macrophage-mediated cytolysis was investigated in a C3H/He mouse-MM46 syngeneic tumor system. GA-treated tumor cells were more susceptible to antibody-dependent macrophage-mediated cytolysis. Lysis of GA-treated cells was observed with less antibody and fewer macrophages than that of untreated cells. However, GA-treated cells had the same amount of antigen sites and the same activity of antibody-dependent attachment to macrophages as do fresh tumor cells. Thus, this enhancement of lysis did not occur at the antibody-binding step or the early step of contact between macrophages and tumor cells but at the subsequent step, perhaps at the tumor lysis step. GA-treated tumor cells could not synthesize DNA or RNA, but cells attenuated with mitomycin C or actinomycin D were not susceptible to antibody-dependent macrophage-mediated cytolysis. These results suggest that the cytostatic effect of GA does not involve change in susceptibility of target cells to lysis, but some impairment of the cell membrane. GA-treated tumor cells could not grow in vivo and could induce antitumor immunity.
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D005976:Glutaral / Q000494:pharmacology*; D007700:Kinetics; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000276:immunology / Q000503:physiopathology*; D051379:Mice; D008815:Mice, Inbred Strains
D000447:Aldehydes; D005976:Glutaral