Induction of resistance to ascites tumor in mice with MFS-180 cells treated with glutaraldehyde, lipopolysaccharide, and concanavalin A.
Immunization with MFS-180 vaccine prepared in the presence of glutaraldehyde (0.05%), lipopolysaccharide (LPS) (100 micrograms/ml), and concanavalin A (Con A) (200 micrograms/ml) could protect Swiss mice against a subsequent challenge by 1 X 10(6) MFS-180 cells. The sequence of attachment of LPS and Con A to glutaraldehyde-treated cells as found to determine the efficacy of the vaccine. LPS coupled with glutaraldehyde-treated cells before Con A could effect 100% survival, while LPS attached after Con A treatment to glutaraldehyde-treated cells showed only 60% survival. The protection was specific for syngeneic cells.
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation; D000951:Antigens, Neoplasm / Q000008:administration & dosage / Q000276:immunology; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology*; D005976:Glutaral / Q000494:pharmacology*; D007114:Immunization; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D012513:Sarcoma, Experimental / Q000276:immunology*
D000447:Aldehydes; D000951:Antigens, Neoplasm; D008070:Lipopolysaccharides; D003208:Concanavalin A; D005976:Glutaral