title: The effects of carbon dioxide and oxygen upon the growth and in vitro transformation of Leishmania mexicana mexicana.
abstract: Growth of Leishmania mexicana promastigotes is highly dependent upon O2 tension. There was a strong positive correlation between the level of O2, growth rate and maximum parasite density. Promastigotes under low oxygen tension decreased in size, protein content and motility, and deaths occurred. Changes in the carbon dioxide concentration (0.1-5.0%) had little effect on promastigote growth. Transformation in vivo of L. mexicana amastigotes to promastigotes also required oxygen, but a low level (0.4%) was sufficient for a high percentage of the amastigotes to transform. At high O2 concentrations, transformation was a little speedier but the number of parasites transforming was little affected. A greater effect was found with CO2. At 5%, transformation was much more rapid than at 0.1% and also an even greater percentage of amastigotes transformed within 48 h. The results give some indication that amastigotes are adpated for growth at low oxygen tensions encountered in vivo and that high carbon dioxide levels may act as a trigger for transformation of the amastigote to promastigote after it is taken up by the sandfly.
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000494:pharmacology*; D007700:Kinetics; D007891:Leishmania / Q000166:cytology / Q000254:growth & development*; D010100:Oxygen / Q000494:pharmacology*
chemical_list: D002245:Carbon Dioxide; D010100:Oxygen