Pulmonary microvascular injury from lipoxygenase infusion: comparison with endotoxemia.
We compared the response of the pulmonary microcirculation to a 5-hr infusion of soybean lipoxygenase with that seen after endotoxin. We monitored microvascular integrity using lung lymph flow QL and lymph/plasma (L/P) protein ratio in unanesthetized sheep. We noted a twofold to threefold increase in QL and a slight decrease in L/P ratio beginning about 1 hr after onset of the lipoxygenase infusion. Leukocyte count decreased significantly and lymph lysosomal enzyme activity increased. Pulmonary artery pressure initially increased from 16 to 31 mm Hg, then decreased to 25 mm Hg during continued infusion. Platelet count remained constant. Parameters returned to baseline several hours after infusion. This pulmonary vascular pressure and lymph flow response was very similar to that seen in the increased permeability phase of endotoxin. A decrease on the L/P ratio and a constant rather than a decreased platelet count were findings different from those seen after endotoxin.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D004731:Endotoxins / Q000097:blood / Q000633:toxicity*; D006976:Hypertension, Pulmonary / Q000139:chemically induced; D008084:Lipoxygenase / Q000633:toxicity*; D008168:Lung / Q000098:blood supply / Q000187:drug effects*; D008196:Lymph / Q000187:drug effects; D008833:Microcirculation / Q000187:drug effects; D012756:Sheep
D001095:Arachidonic Acids; D004731:Endotoxins; D008084:Lipoxygenase