Accumulative effect of DDAVP and heparin in increasing plasma factor VIII levels.
DDAVP (l-desaminocysteine-(8-D-arginine)-vasopressin) produces a marked increase in plasma factor VIII procoagulant (F VIII:C) levels. Previously, we have reported that blood collected into heparin rather than into CPD anticoagulant results in higher starting levels of plasma F VIII:C activity. We therefore wished to determine whether the effects of these two agents were accumulative and whether they would result in any difference in the relative molecular distribution of F VIII:C. Blood was collected into CPD or heparin immediately before and 15 min after an intravenous dose of 0.2 micrograms/kg body weight of DDAVP. Pre-stimulation factor VIII levels were approximately 36% higher in heparinized plasma than in CPD plasma. Following DDAVP stimulation, the final factor VIII activity was increased 3.9-fold when either of the anticoagulants was used, with the heparin sample maintaining a 37% increase over the CPD sample. Column chromatography on Sepharose CL-6B of pre- and post-DDAVP plasma samples collected into either heparin or CPD indicated that there was no change in the relative distribution of the high and low molecular weight forms of F VIII:C. The heparinized sample showed the typical distribution of approximately 60% F VIII:C at void volume (Vo) and 40% at 2.3 Vo, suggesting that DDAVP-stimulated increases of plasma F VIII:C are equally distributed between the carrier and non-carrier associated F VIII:C activities.
D001127:Arginine Vasopressin / Q000494:pharmacology*; D001793:Blood Preservation; D002951:Citrates / Q000494:pharmacology; D003894:Deamino Arginine Vasopressin / Q000008:administration & dosage / Q000494:pharmacology*; D005169:Factor VIII / Q000032:analysis*; D005947:Glucose / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D007275:Injections, Intravenous; D010314:Partial Thromboplastin Time; D010710:Phosphates / Q000494:pharmacology
D002951:Citrates; D010710:Phosphates; D001127:Arginine Vasopressin; D005169:Factor VIII; D006493:Heparin; D003894:Deamino Arginine Vasopressin; D005947:Glucose