B cell helper factors. I. Requirement for both interleukin 2 and another 40,000 mol wt factor.
A helper factor(s) distinct from interleukin 2 (IL-2) was shown to be present in the concanavalin A-stimulated supernatant of normal mouse spleen cells (normal Con A Sn). Spleen cells thoroughly depleted of T cells required both IL-2 and this factor to produce antibody-secreting cells in response to sheep erythrocytes, although in the presence of IL-2 and a few T cells the requirement for the factor was less apparent. The factor had an apparent approximately 40,000 mol wt. The factor was found in normal Con A Sn that had been depleted of IL-2 by absorption with IL-2-dependent T cells and was absent from Con A-stimulated supernatants of the IL-2-producing T cell hybridoma, FS6-14.13. These results indicate that multiple helper factors control the B cell response to antigen and that IL-2, in addition to its T cell growth promoting activity, plays a direct role in B cell responses.
D000818:Animals; D000911:Antibodies, Monoclonal; D000961:Antilymphocyte Serum / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D003208:Concanavalin A / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology; D007375:Interleukin-1; D007376:Interleukin-2 / Q000494:pharmacology*; D008222:Lymphokines / Q000494:pharmacology*; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D008970:Molecular Weight; D011506:Proteins / Q000494:pharmacology*; D011817:Rabbits; D012756:Sheep; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes
D000911:Antibodies, Monoclonal; D000961:Antilymphocyte Serum; D007375:Interleukin-1; D007376:Interleukin-2; D008222:Lymphokines; D011506:Proteins; D003208:Concanavalin A