title: Enrichment for CFU-C from murine and human bone marrow using soybean agglutinin.
abstract: Mouse bone marrow and spleen cells agglutinated by soybean agglutinin (SBA) or peanut agglutinin (PNA) were previously shown to be enriched for spleen colony-forming cells (CFU-S) and sufficiently depleted of graft-versus-host reaction producing cells to allow hematologic reconstitution of lethally irradiated allogeneic recipient mice. A similar enrichment for cells capable of forming colonies in soft agar culture (CFU-C) has now been found in the SBA-agglutinated fraction of mouse bone marrow cells, in contrast to the finding that in human bone marrow the majority of the CFU-C are in the fraction not agglutinated by SBA. Cytofluorometric studies with fluorescein-labeled SBA (FITC-SBA) revealed that the majority of both mouse and human bone marrow cells bind the lectin. Experiments mixing the human marrow fractions separated by SBA reveal that true enrichment for CFU-C is achieved in the unagglutinated fraction, as opposed to a possible depletion of a suppressor cell population. Granulocytic, monocytic, and mixed cell colonies were all enriched in the SBA-unagglutinated cell fraction from human bone marrow.
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D051379:Mice; D037121:Plant Lectins; D030262:Soybean Proteins; D013045:Species Specificity; D013861:Thiocyanates; D010835:Phytohemagglutinins
chemical_list: D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; D013861:Thiocyanates; C028824:soybean lectin; D010835:Phytohemagglutinins