The effect of indomethacin on spontaneous and human menopausal gonadotropin induced pressure changes in the tissue and arterial vascular system from human ovaries perfused in vitro.
Eight human ovaries were perfused in a closed recirculating system with a semisynthetic, hemoglobin-free medium. Arterial and intraovarian pressures were recorded simultaneously. In three experiments spontaneous pressure changes were noticed. In five experiments pressure changes were induced with human menopausal gonadotropin (hMG) (approximately 70mIU/ml follicle stimulating hormone (FSH) and 40mIU/ml luteotrophic hormone (LH) II. IRP-hMG). Frequency and amplitude of pressure changes in the arterial system and fluctuations in tissue pressure were reduced with indomethacin (20 micrograms/ml). In one experiment 40 micrograms/ml indomethacin had been used. In spite of the high dose of indomethacin arterial and tissue pressure changes were not abolished in all experiments. Therefore it is assumed that vascular and tissue activities are not mediated by prostaglandins only.
D000328:Adult; D001158:Arteries / Q000502:physiology; D001794:Blood Pressure / Q000187:drug effects*; D005260:Female; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology*; D008596:Menotropins / Q000494:pharmacology*; D008875:Middle Aged; D009119:Muscle Contraction / Q000187:drug effects; D010053:Ovary / Q000098:blood supply / Q000502:physiology*; D010477:Perfusion; D011312:Pressure
D008596:Menotropins; D007213:Indomethacin