Monoclonal anti-B as a new blood-typing reagent.
Stable cloned lines of anti-B-secreting cells have been derived by fusing spleen cells of a mouse immunized by group B antigen with a mouse myeloma line. The tissue culture supernatant of one of them (NB1/19.112.28) containing secreted monoclonal anti-B antibody is an especially good blood-grouping reagent, well suited for use with manual and automated methods. The unlimited availability of a potent reagent of uniform properties, cheaply produced by a single source, makes monoclonal anti-B a serious competitor to human typing serum.
D000017:ABO Blood-Group System / Q000276:immunology*; D000372:Agglutination Tests; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D000921:Antibody-Producing Cells / Q000276:immunology; D001788:Blood Grouping and Crossmatching; D001793:Blood Preservation; D002478:Cells, Cultured; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology; D051379:Mice
D000017:ABO Blood-Group System; D000906:Antibodies; D000911:Antibodies, Monoclonal