[L-Glutamine transport into synaptosomes isolated from rat brain].
Using a rapid centrifugation-stop procedure, intrasynaptosomal transport of L-glutamine has been measured in conditions allowing the determination of initial rates of this transport. The transport followed saturation kinetics (Km = 0.52 mM; Vmax = 78.24 nmol/min. mg protein), was inhibited at low incubation temperature values and needed a metabolic energy supply. These results demonstrate that L-glutamine is probably transported into synaptic endings via a carrier-mediated mechanism.
D000818:Animals; D001693:Biological Transport, Active; D001921:Brain / Q000378:metabolism*; D002458:Cell Fractionation; D004305:Dose-Response Relationship, Drug; D005973:Glutamine / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000378:metabolism* / Q000502:physiology
D005973:Glutamine
Transport de la l-glutamine dans les terminaisons nerveuses isolées du cerveau du rat.