title: Interferences of mannitol with phosphate determination.
abstract: Methods commonly used for serum phosphate analysis depend upon the combination of inorganic phosphorus with molybdate and subsequent quantitation of the resultant phosphomolybdate by spectrophotometry. This is a case report of mannitol interference with phosphate quantitation by a method not employing dialysis (DuPont ACA) but not with another method which includes a dialysis step (Technicon SMAC). This interference, occurring at an extremely high level of serum mannitol (3.5 g/dl) in a patient with acute renal failure, appears to be due to direct inhibition of the formation of the phosphomolybdate complex by mannitol.
mesh_terms: D001322:Autoanalysis; D003956:Dialysis; D005260:Female; D006801:Humans; D008353:Mannitol / Q000097:blood*; D008875:Middle Aged; D009994:Osmolar Concentration; D010710:Phosphates / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010710:Phosphates; D008353:Mannitol