Cellular adhesion to growth surfaces: a possible role for low molecular weight surface components.
Cell lines initiated from a fibrocystic hyperplasia and a carcinoma of the breast and an established human fetal lung fibroblast line, MRC5, were grown in culture. The surface proteins of the cells were iodinated in cell monolayer, and in suspension following harvesting from culture flasks using EDTA. Data are presented which show that cells labelled in suspension show reduced incorporation of 125I into surface proteins of high molecular weight (greater than 100,000 daltons) and considerably increased incorporation into proteins with molecular weight less than 100,000 daltons. These changes are discussed in relation to the possible release of surface material by the action of EDTA. It is also suggested, on the basis of the current findings, that the detachment of cells may expose low molecular weight components possible involved in the attachment of the cells to the substratum.
D001943:Breast Neoplasms / Q000378:metabolism; D002277:Carcinoma / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D002478:Cells, Cultured; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005347:Fibroblasts; D005348:Fibrocystic Breast Disease / Q000378:metabolism; D006801:Humans; D008168:Lung; D008565:Membrane Proteins / Q000502:physiology*; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis
D008565:Membrane Proteins; D009363:Neoplasm Proteins; D004492:Edetic Acid