Subcellular localization of aldosterone-induced proteins in toad urinary bladders.
Paired toad urinary hemibladders were incubated with [35S]methionine in the presence (experimental) or absence (control) of aldosterone. Short-circuit current was used to monitor aldosterone-induced Na+ transport. Protein synthesis in epithelial cell subcellular fractions (cytosolic, microsomal, mitochondrial) was evaluated by gradient polyacrylamide gel electrophoresis and autoradiography. Aldosterone-induced proteins were identified in the cytosolic and microsomal fractions (70 000 and 15 000 daltons, respectively). These results represent the first demonstration of aldosterone-induced proteins in subcellular fractions of epithelial cells derived from single toad urinary hemibladders.
D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D002024:Bufo marinus / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D008715:Methionine / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D008970:Molecular Weight; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification; D012964:Sodium / Q000378:metabolism; D001743:Urinary Bladder / Q000378:metabolism*
D011506:Proteins; D000450:Aldosterone; D012964:Sodium; D008715:Methionine