title: Proof for the lack of diphtheria toxin in culture filtrates of Corynebacterium ulcerans strain ATCC 9015.
abstract: Extensive efforts to identify the diphtheria toxin in culture filtrates of C. ulcerans strain ATCC 9015 was unsuccessful. The culture filtrates did contain a component (called D-antigen) which formed a precipitin line with commercial diphtheria antitoxin. This component was purified by immunosorbent techniques using immobilized preparations of diphtheria antitoxin. It was shown, that D-antigen has a molecular weight of 84000, is not toxic for guinea-pigs, and has nothing in common with purified diphtheria toxin when tested in gel-diffusion tests. The lack of diphtheria toxin in form of its fragments in culture filtrates concentrated 20-fold was proved by the highly sensitive and specific viroimmunoassay.
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis* / Q000633:toxicity; D001426:Bacterial Proteins / Q000032:analysis; D003352:Corynebacterium / Q000032:analysis* / Q000276:immunology; D004167:Diphtheria Toxin / Q000032:analysis*; D006168:Guinea Pigs; D007118:Immunoassay; D005779:Immunodiffusion; D007163:Immunosorbent Techniques
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004167:Diphtheria Toxin