Sequential expression of preneoplastic and neoplastic characteristics of mouse mammary epithelial cells transformed in organ culture.
Epithelial cells transformed by 7,12-dimethylbenz[a]anthracene (DMBA) in cultures of whole mammary organs of inbred BALB/cAnN female mice were characterized. Nodule-like alveolar lesions (NLAL) induced by DMBA in the mammary gland in vitro after transplantation into gland-free mammary fat pads of syngeneic hosts produced 22 of 44 hyperplastic alveolar outgrowths, and 7 produced mammary carcinomas in the first generation. Enzymatically dissociated mammary cells from glands containing NLAL but no normal alveoli produced 8 hyperplastic outgrowths; 1 of these outgrowths was tumorigenic. Dissociated cells from DMBA-treated glands containing the lobuloalveolar structures produced 26 of 48 hyperplastic alveolar outgrowths, and 8 of these were tumorigenic. Twelve lines of these mammary hyperplasias produced hyperplastic alveolar outgrowths and mammary tumors in a sequential manner up to 8-20 transplant generations. Thus the sequential stages of mammary cells transformed in organ culture are similar to the sequential stages of preneoplasia to neoplasia associated with murine mammary carcinogenesis in vivo.
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced* / Q000473:pathology; D005260:Female; D006965:Hyperplasia / Q000473:pathology; D008325:Mammary Neoplasms, Experimental / Q000209:etiology* / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D009924:Organ Culture Techniques; D011230:Precancerous Conditions / Q000139:chemically induced* / Q000473:pathology; D014184:Transplantation, Homologous
D015127:9,10-Dimethyl-1,2-benzanthracene