Specific enhancement of mouse CFU-E by mouse transferrin.
Pure human and mouse transferrins were prepared by a chromatographic procedure and their effect on the growth of early (BFU-E) and late (CFU-E) erythropoietic precursors in mouse bone marrow is described. In the presence of optimal erythropoietin concentrations mouse bone marrow cells have a greater specificity for mouse transferrin (950 CFU-E colonies/10(5) cells) than human transferrin (650 CFU-E colonies/10(5) cells). Optimal transferrin concentrations for both human and mouse transferrins were 1.3x10(-13)M and 1.3x10(-10)M corresponding to between 7.8x10(7) and 7.8x10(10) molecules/ml of culture. These concentrations are in excess of that calculated on a theoretical basis. Neither erythropoietic burst nor granulocyte/macrophage colony formation exhibited a dose dependent relationship for any of the transferrins employed, although higher colony numbers were obtained with mouse transferrin compared to human transferrin.
D000818:Animals; D002452:Cell Count; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D005779:Immunodiffusion; D051379:Mice; D008810:Mice, Inbred C57BL; D014168:Transferrin / Q000494:pharmacology*
D014168:Transferrin