Resistance of Pseudomonas aeruginosa to modern eye-banking technique.
Rabbit eyes were inoculated with different concentrations of Pseudomonas aeruginosa. Copious irrigation and topical Neosporin solution decreased surface contamination by 90% as compared to controls. Ps. aeruginosa added to M-K medium and stored at 4 degree C remained viable for 6 days and multiplied rapidly when the medium was incubated at 35 degree C. Adding penicillin and streptomycin (125 units/ml each) suppressed, but did not prevent this growth. Gentamicin (100 micrograms/ml) suppressed growth in all vials by 72 hours at 4 degree C. These studies show that gentamicin is clearly superior to penicillin and streptomycin in preventing Ps. aeruginosa contamination of donor corneas. It is suggested that donor eyes and M-K media be routinely cultured upon arrival at the eye-bank and the time of surgery in order to avoid or immediately treat gentamicin-resistant donor cornea contamination.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001414:Bacitracin; D003470:Culture Media; D004338:Drug Combinations / Q000494:pharmacology; D005123:Eye / Q000382:microbiology*; D005839:Gentamicins / Q000494:pharmacology; D009355:Neomycin / Q000494:pharmacology; D009926:Organ Preservation; D010406:Penicillins / Q000494:pharmacology; D011112:Polymyxin B / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D011817:Rabbits; D013307:Streptomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D003470:Culture Media; D004338:Drug Combinations; D005839:Gentamicins; D010406:Penicillins; D001414:Bacitracin; C001816:bacitracin zinc, neomycin sulfate, polymyxin B, drug combination; D009355:Neomycin; D011112:Polymyxin B; D013307:Streptomycin