C3a-induced lysosomal enzyme secretion from human neutrophils: lack of inhibition by f met-leu-phe antagonists and inhibition by arachidonic acid antagonists.
C3a-induced lysosomal enzyme secretion from human peripheral neutrophils in a noncytolytic, dose-dependent (10-100 microgram/ml) process. Release of both primary and secondary granule constituents occurred when neutrophils were exposed to C3a plus cytochalasin B, however, C3 alone induced limited release of lysozyme. A competitive antagonist of the formyl-peptide receptor on neutrophils, t boc (phe-leu) 2-phe, did not block the release induced by C3a. Arachidonic acid antagonists, nordihydroguaiaretic acid and quercetin caused dose-dependent inhibition of release induced by C3a plus cytochalasin B, however, lysozyme release induced by C3a in the absence of cytochalasin B was minimally affected. Indomethacin at high concentration (greater than 10(-5) M) had similar inhibitory effects.
D000706:Anaphylatoxins / Q000502:physiology*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000037:antagonists & inhibitors; D002634:Chemotaxis, Leukocyte; D003176:Complement C3 / Q000502:physiology*; D015926:Complement C3a; D003182:Complement C5 / Q000502:physiology; D015936:Complement C5a; D003571:Cytochalasin B / Q000494:pharmacology; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008247:Lysosomes / Q000201:enzymology*; D009113:Muramidase / Q000378:metabolism; D009239:N-Formylmethionine / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology*; D009842:Oligopeptides / Q000037:antagonists & inhibitors; D010455:Peptides / Q000502:physiology*
D000706:Anaphylatoxins; D001095:Arachidonic Acids; D003176:Complement C3; D003182:Complement C5; D009842:Oligopeptides; D010455:Peptides; D016718:Arachidonic Acid; D003571:Cytochalasin B; D009239:N-Formylmethionine; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015926:Complement C3a; D015936:Complement C5a; D009113:Muramidase; D005966:Glucuronidase