title: Differentiation of ob 17 preadipocytes to adipocytes. Effects of prostaglandin F2alpha and relationship to prostaglandin synthesis.
abstract: The adipose conversion of ob 17 preadipose cells can be irreversibly blocked when prostaglandin F2alpha is included post-confluence for a minimum of 24 h in insulin-containing media. Prostaglandins E1 and E2 are inactive. The lack of adipose conversion is accompanied by the maintenance of a fusiform cell shape, by a slow increase in cell number and by a potent rise in de novo prostaglandin synthesis; it is paralleled by the absence of the characteristic phenotypes of adipose conversion. The multiple effects of prostaglandin F2alpha are dose-dependent, with half-maximal concentrations ranging from 10 to 40 nM. The absence of differentiation and the high rate of prostaglandin synthesis in the presence of prostaglandin F2alpha are likely a consequence of a sustained growth, as also observed with other growth-promoting agents (bovine retinal extract and cat serum). Indomethacin, while suppressing endogenous prostaglandin synthesis, is unable to reverse the long-term and multiple effects of prostaglandin F2alpha. Although adipose conversion normally follows a decrease in prostaglandin production (R. Négrel and G. Ailhaud, Biochem. Biophys. Res. Commun. (1981) 98, 768-777), these results indicate that both events can be dissociated.
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D003066:Coenzyme A Ligases / Q000378:metabolism; D015237:Dinoprost; D015232:Dinoprostone; D004305:Dose-Response Relationship, Drug; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D007213:Indomethacin / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D051379:Mice; D008820:Mice, Obese; D011453:Prostaglandins / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology*
chemical_list: D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D015237:Dinoprost; D005993:Glycerolphosphate Dehydrogenase; D007770:L-Lactate Dehydrogenase; D003066:Coenzyme A Ligases; D015232:Dinoprostone; D007213:Indomethacin