[Basement membrane degradation by enzymes of the 8000-g-lysosome fraction from cervical cancer cells].
By means of immunoelectrophoresis and immunodiffusion after Ouchterlony the spectrum of lysosomal enzymes in the 8000-g-fraction of invasive cervical carcinoma cells and of normal human lymphocytes was investigated. A glomerulobasement membrane preparation (GBM) was used as substrate, and the action was characterized by inhibition studies. The proteases of cervical carcinoma cells and of lymphocytes caused a GBM degradation into identical antigenic pieces. While o-phenanthroline, 5-fluorouracil and HgCl2 showed no enzymatic inhibition, EDTA as well as aprotinine inhibited partially in all cases combined. EDTA and aprotinine totally stopped the reaction. While normal human serum revealed total inhibition of lysosomal enzymes of lymphocytes, the proteases of cervical carcinoma cells only were inhibited partially at all concentrations. This defective inhibitions is discussed concerning its in vivo significance for invasion.
D001485:Basement Membrane / Q000201:enzymology; D005260:Female; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007678:Kidney Glomerulus / Q000201:enzymology*; D008214:Lymphocytes / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology*; D002583:Uterine Cervical Neoplasms / Q000201:enzymology*
D010447:Peptide Hydrolases
Basalmembranabbau durch Enzyme der 8000-g-Lysosomen-Fraktion von Zervixkarzinomzellen.