Rat intestinal microvillus membrane sucrase-isomaltase is a single high molecular weight protein and fully active enzyme in the absence of luminal factors.
Sucrase-isomaltase immunoprecipitated from brush border of an intestinal transplant lacking pancreatic proteases was found to be a single, high molecular weight protein. Elastase digestion converted this protein into two subunits which co-migrated on electrophoresis with those normally found on the microvillus membrane. The high molecular weight form had full sucrase and isomaltase activities.
D000818:Animals; D005779:Immunodiffusion; D007422:Intestines / Q000196:embryology / Q000201:enzymology* / Q000637:transplantation; D007700:Kinetics; D046911:Macromolecular Substances; D008871:Microvilli / Q000201:enzymology; D009097:Multienzyme Complexes / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology; D010196:Pancreatic Elastase / Q000378:metabolism; D010447:Peptide Hydrolases / Q000502:physiology; D051381:Rats; D013394:Sucrase-Isomaltase Complex / Q000378:metabolism*
D046911:Macromolecular Substances; D009097:Multienzyme Complexes; D013394:Sucrase-Isomaltase Complex; D010447:Peptide Hydrolases; D010196:Pancreatic Elastase