[Demonstration of neuropeptides in cultured neuroretinal cells of chick embryo].
Immunoreactive neuropeptides have been found in cultures of neuroretina cells obtained from 7 day Chick embryos. Methionine-enkephalin and thyroliberin (TRH) were present in a large number of neurons, while somatostatin, beta-melanotropin (MSH) and alpha-endorphin were occasionally seen in a few neurons. In cultures devoid of neurons and probably made of Müller (astroglial) cells, methionine-enkephalin, alpha-MSH and TRH were each detected in 60 to 80% of the cells. Immunoreactive methionine enkephalin was also localized in Müller cells in retina sections of 10 and 20 day old embryos.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004723:Endorphins / Q000032:analysis*; D004744:Enkephalin, Methionine; D004745:Enkephalins / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D009074:Melanocyte-Stimulating Hormones / Q000032:analysis; D009474:Neurons / Q000032:analysis*; D012160:Retina / Q000166:cytology*; D013973:Thyrotropin-Releasing Hormone / Q000032:analysis*
D004723:Endorphins; D004745:Enkephalins; D004744:Enkephalin, Methionine; D013973:Thyrotropin-Releasing Hormone; D009074:Melanocyte-Stimulating Hormones
Mise en évidence de neuropeptides dans des cultures de cellules neurorétiniennes d'embryon de poulet.