The location of arabinosyl:hydroxyproline transferase in the membrane system of potato tissue culture cells.
Incubation of a particulate preparation from potato tissue culture cells with UDP-beta-L-[1-3H] arabinose yielded a glycoprotein fraction containing labelled material with the characteristics of hydroxyproline arabinosides. The sugar-protein linkage was resistant to hot alkaline hydrolysis, and the hydrolytic products showed similar electrophoretic and chromatographic behavior to authentic hydroxyproline-arabinosides prepared from potato tissue culture cell walls. Incorporation of arabinose into glycoprotein was stimulated by the addition of de-arabinosylated potato lectin. The product of the incubation co-migrated with native potato lectin on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. The subcellular distribution of the arabinosyl-transferase was investigated by fractionating potato tissue culture membranes on a discontinuous sucrose gradient in the presence or absence of Mg2+. Under both fractionation conditions the highest specific activity of the enzyme was found in the Golgi-enriched fraction. The results are discussed in relation to the synthesis of the hydroxy-proline-rich glycoprotein component of plant cell walls.
D001089:Arabinose / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D002478:Cells, Cultured; D004492:Edetic Acid / Q000494:pharmacology; D006027:Glycosides / Q000378:metabolism; D006909:Hydroxyproline / Q000031:analogs & derivatives / Q000378:metabolism; D037102:Lectins / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D010430:Pentosyltransferases / Q000378:metabolism*; D037121:Plant Lectins; D010944:Plants / Q000187:drug effects / Q000201:enzymology*; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology
D006027:Glycosides; D037102:Lectins; D037121:Plant Lectins; D004492:Edetic Acid; D001089:Arabinose; D010430:Pentosyltransferases; C033081:arabinosyl-hydroxyproline transferase; D008274:Magnesium; D006909:Hydroxyproline