title: [Immunohistology of the antibody-formation against M-protein of Streptococcus pyogenes in Macaca mulatta (author's transl)].
abstract: The immunohistology of the antibody synthesis against M proteins of Streptococcus pyogenes (group A streptococci) was studied in rhesus monkeys (Macaca mulatta). For the immunoelectron microscopic detection of antibody producing cells peroxidase-labelled M protein was used. Cryostat sections of glutaraldehyde-fixed lymphoid organs were incubated with the peroxidase-conjugate followed by 3,3'-diaminobenzidine and H2O2. The labelled tissue sections were further processed for the electron microscopy. Two sites of specific labelling were observed: 1) The cell membrane of lymphocytes and transformed lymphocytes and 2) the endoplasmic reticulum of plasma cells, proplasmocytes and transformed lymphocytes.
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000276:immunology*; D002352:Carrier Proteins; D002462:Cell Membrane / Q000276:immunology; D004721:Endoplasmic Reticulum / Q000276:immunology; D007124:Immunoenzyme Techniques; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D008253:Macaca mulatta; D008854:Microscopy, Electron; D010950:Plasma Cells / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; C014689:streptococcal M protein
vernacular_title: Immunhistologie der Antikörperbildung gegen M-Protein von Streptococcus pyogenes bei Macaca mulatta.