S-Adenosylhomocysteine hydrolase activity in a lymphoblastoid cell line from a patient with adenosine deaminase dificiency disease.
S-Adenosylhomocysteine (S-AdoHcy) hydrolase activity in a lymphoblastoid cell line from a patient with adenosine deaminase deficiency disease (ADA(-)LCL) was found to be approximately 60% of that in ADA (+)lymphoblastoid cell lines. S-AdoHcy hydrolase of ADA(-)LCL was more sensitive to inhibition by 2'-deoxyadenosine as compared with that of ADA(+)LCL. The inhibitory effect of 2'-deoxyadenosine was evident in cell growth and immunoglobulin production of lymphoblastoid cell lines.
D000243:Adenosine Deaminase / Q000172:deficiency*; D043383:Adenosylhomocysteinase; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003839:Deoxyadenosines / Q000494:pharmacology; D003841:Deoxycytidine / Q000494:pharmacology; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D007136:Immunoglobulins / Q000096:biosynthesis; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology; D009700:Nucleoside Deaminases / Q000172:deficiency*; D011686:Purine-Pyrimidine Metabolism, Inborn Errors / Q000201:enzymology*
D003839:Deoxyadenosines; D007136:Immunoglobulins; D003841:Deoxycytidine; D006867:Hydrolases; D043383:Adenosylhomocysteinase; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase