Improved fluorometric-iodine method for determination of aflatoxin in corn.
Several innovations in the fluorometric-iodine (FL-I) method for determining aflatoxin in corn made the procedure faster and more convenient than the original method. Ground corn was packed into a chromatographic column and the aflatoxins were extracted with methylene chloride. Sep-Pak C18 cartridges were used for sample cleanup. A 5 min thin layer chromatographic slide analysis confirmed the presence of aflatoxin, which was quantitated by fluorometric determinations of the iodine derivative of aflatoxin B1.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000032:analysis*; D007455:Iodine; D013050:Spectrometry, Fluorescence / Q000379:methods; D003313:Zea mays / Q000032:analysis*
D000348:Aflatoxins; D007455:Iodine; D016604:Aflatoxin B1