title: Studies on the inhibition of highly purified calf thymus 8S and 7.3S DNA polymerase alpha by aphidicolin.
abstract: On activated DNA aphidicolin competitively inhibits the incorporation of dCMP by both calf thymus DNA polymerase alpha A2 and C enzymes and inhibits the incorporation of the other three deoxynucleoside monophosphates apparently non-competitively. However, aphidicolin does not inhibit the incorporation of dAMP into poly(dT) . oligo(A)10 nor does it inhibit the incorporation of dGMP into poly(dC) . oligo(dG)10, but, it does competitively inhibit the incorporation of dTMP into poly(dA) . oligo(dT)10.
mesh_terms: D000818:Animals; D016590:Aphidicolin; D002250:Carbon Radioisotopes; D002417:Cattle; D004247:DNA; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors*; D003854:Deoxyribonucleotides; D004224:Diterpenes / Q000494:pharmacology*; D007700:Kinetics; D008970:Molecular Weight; D019384:Nucleic Acid Synthesis Inhibitors; D011865:Radioisotope Dilution Technique; D013950:Thymus Gland / Q000201:enzymology*; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D003854:Deoxyribonucleotides; D004224:Diterpenes; D019384:Nucleic Acid Synthesis Inhibitors; D014316:Tritium; D016590:Aphidicolin; D004247:DNA; D004257:DNA Polymerase II