Metabolic activation of a tryptophan pyrolysis product, 3-amino-1-methyl-5H-pyrido[4,3-b]indole(TRP-P-2) by isolated rat liver nuclei.
The metabolic activation of a tryptophan pyrolysis product, 3-amino-1-methyl-5H-pyrido[4,3-b]indole(Trp-P-2), by rat liver nuclei was studied. Nuclei from the livers of rats treated with polychlorinated biphenyl (PCB) or 3-methylcholanthrene (MC) showed high mutagenic activity with Trp-P-2 in the Ames test, but activities with nuclei of untreated or phenobarbital (PB)-treated rat livers were quite low. The formation of N-hydroxy-Trp-P-2 by nuclei of PCB- or MC-treated rat livers was greater than that by nuclei of untreated or PB-treated rat livers. Similar results were observed with microsomes, which suggests that Trp-P-2 is metabolized by the same type of monoxygenase system in nuclei as in microsomes.
D000818:Animals; D001711:Biotransformation; D002243:Carbolines / Q000378:metabolism* / Q000494:pharmacology; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology; D066298:In Vitro Techniques; D007211:Indoles / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008748:Methylcholanthrene / Q000494:pharmacology; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D011078:Polychlorinated Biphenyls / Q000494:pharmacology; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D014365:Tryptophan Hydroxylase / Q000378:metabolism
D002243:Carbolines; D007211:Indoles; D008748:Methylcholanthrene; C019133:3-amino-1-methyl-5H-pyrido(4,3-b)indole; D011078:Polychlorinated Biphenyls; D014365:Tryptophan Hydroxylase