Factors affecting acid alpha-naphthyl acetate esterase activity in peripheral blood lymphocytes.
Pre-staining conditions that affect alpha-naphthyl acetate esterase (ANAE) activity in peripheral blood lymphocytes have been evaluated. The effects of different fixatives, pH of fixation, pre-fixation storage time and length of fixation have been compared with regard to maximal preservation of ANAE activity in peripheral T-cells.
D000328:Adult; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D005404:Fixatives; D005976:Glutaral; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008875:Middle Aged; D009283:Naphthol AS D Esterase / Q000097:blood*; D013601:T-Lymphocytes / Q000201:enzymology*; D013997:Time Factors
D005404:Fixatives; D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases; D005976:Glutaral