Low molecular weight RNAs transcribed in vitro by RNA polymerase III from Alu-type dispersed repeats in Chinese hamster DNA are also found in vivo.
An Alu-type dispersed repeat previously identified in a cloned fragment of Chinese hamster DNA [Haynes, S. R., Toomey, T. P., Leinwand, L. & Jelinek, W. R. (1981) Mol. Cell. Biol. 1, 573-583] serves as a template for cell-free transcription of discrete low molecular weight RNAs by RNA polymerase III [RNA nucleotidyltransferase (DNA-directed), EC 2.7.7.6]. A class of analogous RNAs has been isolated from growing Chinese hamster cells by hybridization of total low molecular weight nuclear RNAs to the cloned DNA fragment from which cell-free transcription occurs. Two-dimensional analysis of RNase digestion products of these RNAs suggests that they are transcribed from multiple members of the Alu-type dispersed repeat family.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics*; D004274:DNA, Recombinant / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005260:Female; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D009843:Oligoribonucleotides / Q000032:analysis; D010053:Ovary; D012320:RNA Polymerase III / Q000378:metabolism*; D006163:Ribonuclease T1; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D009843:Oligoribonucleotides; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D012320:RNA Polymerase III; D006163:Ribonuclease T1