A monoclonal antibody that recognizes B cells and B cell precursors in mice.
The monoclonal antibody, RA3-2C2, appears to be specific for cells within the B cell lineage. This antibody does not recognize thymocytes, peripheral T cells, or nonlymphoid hematopoietic cells in the spleen or bone marrow. Nor does it recognize the pluripotent hematopoietic stem cells, the spleen colony-forming unit, All sIg+ B cells and most plasma cells are RA3-2C2+. In addition, approximately 20% of nucleated bone marrow cells are RA3-2C2+ but sIg-. This population contains B cell precursors that can give rise to sIg+ cells within 2 d in vitro.
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology*; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002459:Cell Fusion; D005260:Female; D005455:Fluorescent Antibody Technique; D007075:Immunoglobulin M / Q000096:biosynthesis; D008027:Light; D008221:Lymphoid Tissue / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D051381:Rats; D011917:Rats, Inbred Lew; D011947:Receptors, Antigen, B-Cell; D012542:Scattering, Radiation; D012756:Sheep
D000906:Antibodies; D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell