The isolation and characterization of a 1,2-propanediol oxidoreductase from Neisseria gonorrhoeae.
An enzyme which oxidizes 1,2-propanediol in the presence of NAD+ has been purified from lysates of Neisseria gonorrhoeae. The enzyme was activated by monovalent cations, had a pH optimum between 9 and 10, and showed a substrate specificity unlike any known alcohol or glycerol dehydrogenase. The enzyme had an apparent Km of 17 mM for 1,2-propanediol and 0 . 37 mM for NAD+. When chromatographed on a Sephadex G-150 column, the enzyme eluted as a single peak in the molecular weight region of a bovine serum albumin marker. An antibody to the purified enzyme was prepared in goats. When antiserum was reacted with the enzyme in immunodiffusion experiments, a single precipitin band was detected. When the enzyme was mixed with an excess of antibody and then reacted with substrate, enzyme activity was completely inhibited.
D000429:Alcohol Oxidoreductases / Q000276:immunology / Q000302:isolation & purification*; D000937:Antigen-Antibody Reactions; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D007700:Kinetics; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D009344:Neisseria gonorrhoeae / Q000201:enzymology*; D011409:Propylene Glycols / Q000378:metabolism
D011409:Propylene Glycols; D009243:NAD; D000429:Alcohol Oxidoreductases