Measurement of intracellular free calcium in monkey kidney cells with aequorin.
A method has been developed for the measurement of intracellular free calcium in mammalian cells. The calcium-sensitive photoprotein aequorin can be incorporated into isolated cells by hypo-osmotic treatment without altering the cell viability, permeability, or metabolism. Intracellular calcium activity (Cai2+) was monitored in a perfusion system. In monkey kidney cells (LLC-MK2), Cai2+ is approximately 57 nanomoles per liter. Changes in Cai2+ with time can also be followed: exposure of the cells to anaerobiosis or the calcium ionophore A23187 reversibly increases Cai2+. The method has also been successfully tested in rat hepatocytes.
D000331:Aequorin; D000693:Anaerobiosis; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008164:Luminescent Proteins; D008253:Macaca mulatta
D008164:Luminescent Proteins; D000001:Calcimycin; D000331:Aequorin; D002118:Calcium