Enzymic coupling of acylhydrolase and prostaglandin synthase activities in subcellular fractions from rabbit renal medulla.
We have recently shown that mitochondrial and plasma-membrane fractions from kidney medulla possess Ca(2+)-stimulated acylhydrolase and prostaglandin synthase activities. The nature of the enzymic coupling between the Ca(2+)-stimulated arachidonic acid release and its subsequent conversion into prostaglandins was investigated in subcellular fractions from rabbit kidney medulla. Plasma-membrane, mitochondrial and microsomal fractions were found to have similar apparent K(m) values for conversion of added exogenous arachidonate into prostaglandins. The rate of prostaglandin biosynthesis (V(max.)) from added arachidonic acid in the microsomal fraction was approx. 2-fold higher than in the other subcellular fractions. In contrast, prostaglandin E(2) synthesis from endogenous arachidonate in plasma-membrane and mitochondrial fractions was 3-4-fold higher than in microsomes. Furthermore, Ca(2+) stimulated endogenous arachidonate deacylation and prostaglandin E(2) generation in the former two fractions but not in microsomes. In mitochondrial or crude plasma-membrane fractions, in which prostaglandin biosynthesis was inhibited with aspirin, arachidonate released from these fractions was converted into prostaglandins by the microsomal prostaglandin synthase. Thus an intracellular prostaglandin generation process that involves inter-fraction transfer of arachidonic acid can operate. Prostaglandin generation by such an inter-fraction process is, however, less efficient than by an intra-fraction process, where arachidonic acid released by mitochondria or crude plasma membranes is converted into prostaglandins by prostaglandin synthase present in the same fraction. This demonstrates the presence of a tight intra-fraction enzymic coupling between Ca(2+)-stimulated acylhydrolase and prostaglandin synthase enzyme systems in both mitochondrial and plasma-membrane fractions.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001241:Aspirin / Q000494:pharmacology; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D015232:Dinoprostone; D005227:Fatty Acids / Q000378:metabolism; D066298:In Vitro Techniques; D007679:Kidney Medulla / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D011458:Prostaglandins E / Q000096:biosynthesis; D011817:Rabbits; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology
D001095:Arachidonic Acids; D005227:Fatty Acids; D011458:Prostaglandins E; D016718:Arachidonic Acid; D011451:Prostaglandin-Endoperoxide Synthases; D002265:Carboxylic Ester Hydrolases; D015232:Dinoprostone; D001241:Aspirin