The use of a simple method to avoid cell shrinkage during SEM preparation.
In the course of preparing specimens for scanning electron microscopy, both glutaraldehyde and OsO4-fixed cells exhibit a considerable shrinkage with a reduction of the mean cellular diameter of about 45% after critical point drying. However, if cells are successively treated with glutaraldehyde, OsO4, tannic acid and uranyl acetate solutions, cellular shrinkage of only 5% is observed.
D000818:Animals; D003584:Cytological Techniques; D005976:Glutaral; D047348:Hydrolyzable Tannins; D008214:Lymphocytes / Q000648:ultrastructure; D008264:Macrophages / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D009942:Organometallic Compounds; D009993:Osmium Tetroxide; D014501:Uranium
D047348:Hydrolyzable Tannins; D009942:Organometallic Compounds; C005460:uranyl acetate; D014501:Uranium; D009993:Osmium Tetroxide; D005976:Glutaral