Release of colicin E2 from Escherichia coli.
Treatment of Escherichia coli K-12(ColE2.P9) with 500 ng of mitomycin C per ml resulted in rapid and almost synchronous colicin E2 production. Colicin accumulated outside the cytoplasmic membrane, most probably in the periplasmic space. Colicin release occurred during a period in which the turbidity of the culture declined markedly. Periplasmic alkaline phosphatase was released during the same period, but cytoplasmic beta-galactosidase release was delayed.
D000469:Alkaline Phosphatase / Q000378:metabolism; D003087:Colicins / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D008937:Mitomycins / Q000494:pharmacology; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D001616:beta-Galactosidase / Q000378:metabolism
D003087:Colicins; D008937:Mitomycins; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D017830:Octoxynol; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase