Micromethod for lipid-chromatographic determination of cholesterol in lipemic sera.
A micromethod involving "high-performance" lipid chromatography was developed for determining cholesterol in sera obtained from animals that has been infused with high doses of lipid emulsions. Only 50 micro L of serum is required, and there is no interference from turbidity, bilirubin, fat-soluble vitamins, and sterols. Correlation with the commonly used Abell-Kendall method was good at cholesterol concentrations as great as 10.0 g/L (r = 0.998). The assay is ideal for determinations on samples from pediatric and hyperlipidemic patients. It is particularly useful for monitoring patients on lipid-emulsion therapy and for toxicological studies with small animals.
D000818:Animals; D002648:Child; D002784:Cholesterol / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005217:Fat Emulsions, Intravenous; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood*; D008722:Methods; D010288:Parenteral Nutrition; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013552:Swine; D014116:Toxicology
D005217:Fat Emulsions, Intravenous; D002784:Cholesterol