DNA repair in nuclei isolated from HeLa cells.
We have studied the DNA repair synthesis in HeLa cell isolated nuclei in presence of aphidicolin, which selectively inhibits DNA polymerase alpha but not DNA polymerases beta and gamma. The drug strongly depresses the DNA synthesis rate both in vivo and in the subcellular system but seems not to interfere with DNA repair. The enhancement in nucleotide incorporation by isolated nuclei after UV-irradiation is observed only in the presence of ATP.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D016590:Aphidicolin; D002453:Cell Cycle; D002467:Cell Nucleus / Q000378:metabolism* / Q000528:radiation effects; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects; D004259:DNA-Directed DNA Polymerase / Q000502:physiology; D004224:Diterpenes / Q000494:pharmacology*; D006367:HeLa Cells / Q000187:drug effects / Q000528:radiation effects*; D006801:Humans; D008274:Magnesium / Q000494:pharmacology; D019384:Nucleic Acid Synthesis Inhibitors; D014466:Ultraviolet Rays
D004224:Diterpenes; D019384:Nucleic Acid Synthesis Inhibitors; D016590:Aphidicolin; D000255:Adenosine Triphosphate; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D008274:Magnesium