[Inhibitory effect of cytembena (sodium-cis-beta-methoxybenzoyl-beta-bromoacrylate) on the cell kinetics of HeLa cells in culture].
The inhibiting effect of Cytembena on HeLa cell kinetics has been demonstrated and analyzed. The percentage of cycling cells decreases, according to the concentration, between 7.5 and 2.5 x 10(-5) M. Estimation of DNA by cell flow cytophotometry shows an important shift in the distribution of cycling cells with a relative decrease of G1 cells and a very important accumulation of G2 cells. According to our experimental conditions, the blocking up in G2 is irreversible only at 7.5 x 10(-5) M.
D000179:Acrylates / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006367:HeLa Cells / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007700:Kinetics
D000179:Acrylates; D004273:DNA, Neoplasm; C100245:bromebric acid
Effet inhibiteur du Cytembena (cis-beta-methoxybenzoyl-beta-bromacrylate de sodium) sur la cinétique des cellules HeLa en culture.