Effects of sequential exposure to aflatoxin B1 and diethylnitrosamine on vascular and stomach tissue and additional target organs in rats.
Male weanling rats were given a standard amount (375 micrograms) of aflatoxin B1; some were then exposed to dietary diethylnitrosamine (DEN) in varying concentrations ranging from 5 to 80 ppm for 20 weeks. They were maintained for an additional period of time following DEN exposure and then killed for gross and microscopic examination. DEN at 5 ppm did not enhance aflatoxin B1 liver carcinogenesis. Ten ppm or more of DEN, however, enhanced liver carcinogenesis and introduced two additional target organs; angiosarcomas of the lung and squamous cell carcinomas of the stomach types of tumors which have not been observed after treatment with DEN alone, were noted.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000008:administration & dosage*; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced; D003043:Cocarcinogenesis; D004052:Diethylnitrosamine / Q000008:administration & dosage*; D006394:Hemangiosarcoma / Q000139:chemically induced; D008113:Liver Neoplasms / Q000139:chemically induced; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced; D008175:Lung Neoplasms / Q000139:chemically induced; D008297:Male; D009374:Neoplasms, Experimental / Q000139:chemically induced; D009602:Nitrosamines / Q000008:administration & dosage*; D051381:Rats; D013274:Stomach Neoplasms / Q000139:chemically induced*; D013997:Time Factors
D000348:Aflatoxins; D009602:Nitrosamines; D004052:Diethylnitrosamine; D016604:Aflatoxin B1