Human lymphocytes with either the OKT4 or OKT8 phenotype produce interleukin 2 in culture.
In this study, we demonstrate that both highly purified T4+ and T8+ lymphocytes can produce substantial amounts of Interleukin 2(IL 2) when stimulated with the combination of concanavalin A (Con A) and phorbol myristate acetate. Furthermore, addition of IL 1 to macrophage-depleted T lymphocytes significantly increased IL 2 production by lymphocytes of either the T4+ or T8+ phenotype. These findings provide a basis for further studies of the molecular mechanisms involved in human immune cell interactions.
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D007375:Interleukin-1; D007376:Interleukin-2 / Q000096:biosynthesis*; D008222:Lymphokines / Q000096:biosynthesis*; D010641:Phenotype; D010835:Phytohemagglutinins / Q000494:pharmacology; D011506:Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism*
D007375:Interleukin-1; D007376:Interleukin-2; D008222:Lymphokines; D010835:Phytohemagglutinins; D011506:Proteins; D003208:Concanavalin A