Mode of degradation of tRNAs with ozone.
Ozone-treatment of tRNA resulted in the degradation of guanine residues located on the loop regions, such as the anticodon and D-loop region. In addition, it became evident that the guanine residues in the consecutive sequences, such as G-G-G-G and G-G-m1G in tRNAPro, were the most susceptive to the ozone-treatment. The internucleotidic linkage of the treated tRNA was not cleaved but several fragments were obtained by a gel electrophoretic separation after heating at 60% in 1M aniline-acetate buffer (pH 4.5). Major fragments derived from tRNAPro were the 3'-half and the 5'-half molecules produced by the cleavage at the anticodon region.
D000926:Anticodon; D001483:Base Sequence; D006147:Guanine; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000032:analysis; D010126:Ozone; D012343:RNA, Transfer; D006163:Ribonuclease T1
D000926:Anticodon; D009843:Oligoribonucleotides; D006147:Guanine; D010126:Ozone; D012343:RNA, Transfer; D006163:Ribonuclease T1