[Ratio of the mutation spectrum to the physiological state of the Penicillium chrysogenum cell. Auxotrophic mutations induced by UV rays at different stages of DNA replication].
The spectrum of the mutations obtained with exposure of nonactivated spores of Pen. chrysogenum to N-nitrozo-N-methylbiuret (NMB) was studied and it was shown that the mutations associated with blocking of amino acid synthesis predominated. When activated spores of Pen. chrysogenum were treated with the mutagen, correlation was observed between the spectrum of the induced mutations, the physiological state of the cells and the exposure time. It is supposed that the mutagen molecule affects the replicating part of the DNA molecule. Schemes for predominating liberation of the mutations according to the markers in time were developed. This may give an idea of the order of the markers in genome.
D004261:DNA Replication / Q000528:radiation effects*; D004271:DNA, Fungal / Q000096:biosynthesis*; D004307:Dose-Response Relationship, Radiation; D009154:Mutation; D010407:Penicillium / Q000528:radiation effects*; D010408:Penicillium chrysogenum / Q000502:physiology / Q000528:radiation effects*; D013997:Time Factors; D014466:Ultraviolet Rays
D004271:DNA, Fungal
Zavisimost' spektra mutatsii of fiziologicheskogo sostoianiia kletki Penicillium chrysogenum. Auksotrofnye mutatsii, indutsirovannye UF-luchami v ravlichnye stadii replikatsii DNK.