[Viscosimetric evaluation of DNA fragmentation induced in vivo by alkylating agents].
A sensitive viscosimetric approach for measuring DNA single-strand breaks induced by low doses of alkylating agents is presented. The assay is based on the breaks-induced increasing rate of strand separation of rat liver DNA in alkaline solution (pH 12,5; 22 C). Because of the strong dependence of viscosity on DNA unwinding, measurement of time-course of viscosity increase can be used to detect small breakage of DNA. The kinetics of DNA denaturation has been also studied under two different alkaline conditions.
D000477:Alkylating Agents / Q000009:adverse effects*; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects; D004277:DNA, Single-Stranded; D005998:Glycine / Q000009:adverse effects; D008099:Liver / Q000187:drug effects*; D008297:Male; D051381:Rats; D014783:Viscosity
D000477:Alkylating Agents; D004277:DNA, Single-Stranded; D005998:Glycine
Valutazione viscosimetrica della frammentazione del DNA indotta "in vivo" da agenti alchilanti.