[Effect of trypsin on the cell surface : influence on the lysosomal exocytosis of alveolar macrophages of the guinea pig].
Secretion of the main lysosomal hydrolase, N-acetyl-beta-D-glucosaminidase of Guinea Pig alveolar macrophages was studied in vitro; without stimulus of phagocytosis or pinocytosis, glycosidase secretion was increased by trypsin. Enzymatic activity secreted under trypsinisation was heterogeneous as compared to the enzymatic activity secreted by control cells: under incubation with Concanavalin A (Con A), glycosidase activity secreted by trypsinised cells decreased by 30%, whereas Concanavalin A did not alter enzymatic activity secreted by control cells.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D005089:Exocytosis / Q000187:drug effects*; D006168:Guinea Pigs; D008247:Lysosomes / Q000201:enzymology*; D008264:Macrophages / Q000201:enzymology*; D011650:Pulmonary Alveoli / Q000201:enzymology*; D014357:Trypsin / Q000494:pharmacology*
D003208:Concanavalin A; D000118:Acetylglucosaminidase; D014357:Trypsin
Action de la trypsine sur la surface cellulaire : influence sur l'exocytose lysosomiale des macrophages alvéolaires de cobaye.