[Rubredoxin reductase in crude extracts of Acinetobacter calcoaceticus in relation to carbon source and growth phase].
By immunization of rabbits with discelectrophoretically purified rubredoxin-reductase from Ac. calcoaceticus an antiserum against this enzyme was prepared. The antiserum was monospecifical to a diaphoretic activity, which had a RF-value identical with the rubredoxin-reductase-RF value. It has been shown by discelectrophoresis, immunoelectrophoresis, OUCHTERLONY technique and kinetic investigations that crude extracts of bacteria grown on C16, malate, succinate or acetate contain rubredoxin-reductase. By the LAURELL technique we demonstrated that the amount of the enzyme is nearly independent of the carbon source. There are only differences during the different growth phases.
D000085:Acetates / Q000378:metabolism; D000150:Acinetobacter / Q000201:enzymology* / Q000254:growth & development; D000473:Alkanes / Q000378:metabolism; D004589:Electrophoresis, Disc; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008293:Malates / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D012416:Rubredoxins / Q000378:metabolism; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid
D000085:Acetates; D000473:Alkanes; D008293:Malates; D012416:Rubredoxins; D013386:Succinates; C030298:malic acid; D019802:Succinic Acid; C023091:rubredoxin-NAD+ reductase; D009247:NADH, NADPH Oxidoreductases; C007932:n-hexadecane
Rubredoxin-Reductase in Rohextrakten von Acinetobacter calcoaceticus in Abhängigkeit von C-Quelle und Wachstumsphase.