Effects of acute hypoxia on incorporation of [1-14C]arachidonic into glycerolipids of rat brain.
The effects of 1 min of acute hypoxic treatment (1% O2 in N2) on incorporation of [1-14C]arachidonic acid into brain lipids of 16-day-old rats were investigated at 3, 6, and 12 min after intracerebral injection of the labeled fatty acid. The hypoxic-hypoxia condition associated with convulsive seizures caused a decrease in the conversion of labeled arachidonate to its acyl-CoA as well as incorporation of the label into the brain phospholipids. Among the phospholipids, there was a specific decrease in the labeling of diacylglycerophosphoinositol (GPI), and this change was accompanied by an increase in labeling of the diacylglycerols. These results indicate that metabolism of the long-chain fatty acids and some glycerolipids in brain are vulnerable to acute hypoxic treatment.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005989:Glycerides / Q000096:biosynthesis*; D000860:Hypoxia / Q000378:metabolism*; D007700:Kinetics; D010712:Phosphatidic Acids / Q000096:biosynthesis*; D010743:Phospholipids / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D012640:Seizures / Q000378:metabolism
D001095:Arachidonic Acids; D005230:Fatty Acids, Nonesterified; D005989:Glycerides; D010712:Phosphatidic Acids; D010743:Phospholipids; D016718:Arachidonic Acid