Rapid fractionation of human high density apolipoproteins by high performance liquid chromatography.
A simple and rapid fractionation procedure (30 min) has been developed for the isolation of the major apoproteins from human serum high density lipoproteins by molecular sieving in a high performance liquid chromatographic column. Apo A-I, apoA-II and the C peptides are quantitatively resolved up to a protein load of 3 mg. The technique has also been successfully applied to the final purification of A apoproteins which had been isolated by conventional chromatographic procedures and as a sensitive analytical tool for assessing apoprotein purity.
D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D001053:Apolipoproteins / Q000302:isolation & purification; D002096:C-Peptide / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007158:Immunologic Techniques; D008075:Lipoproteins, HDL / Q000097:blood / Q000302:isolation & purification*
D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D001053:Apolipoproteins; D002096:C-Peptide; D008075:Lipoproteins, HDL