Induction of epidermal growth factor by tri-iodo-L-thyronine in the submandibular glands of mice with testicular feminization.
The content of epidermal growth factor (EGF) as a high molecular weight complex (HMW-EGF) in the submandibular glands of mice was measured simply by a single radial immunodiffusion method. In female mice, the amount of HMW-EGF was increased 10-fold by tri-iodo-L-thyronine (T3) and 60-fold by 5 alpha-dihydrotestosterone (5 alpha-DHT). In mice with testicular feminization (Tfm), which are genetically deficient in androgen receptor, T3 but not 5 alpha-DHT increased the HMW-EGF from a non-detectable level to 5.4 +/- 0.94 micrograms/mg protein. It was concluded that EGF is also synthesized under the control of thyroid hormone in vivo, and that androgen was not involved in this induction of EGF by thyroid hormone.
D013734:Androgen-Insensitivity Syndrome / Q000378:metabolism*; D000818:Animals; D013196:Dihydrotestosterone / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000096:biosynthesis*; D005260:Female; D005779:Immunodiffusion; D007700:Kinetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012737:Sex Factors; D013363:Submandibular Gland / Q000187:drug effects / Q000378:metabolism*; D014284:Triiodothyronine / Q000494:pharmacology*
D014284:Triiodothyronine; D013196:Dihydrotestosterone; D004815:Epidermal Growth Factor