title: Reactivity of mitomycin C with synthetic polyribonucleotides containing guanine or guanine analogs.
abstract: The guanine residues in nucleic acids are believed to be the major covalent binding site of the antibiotic mitomycin C. To identify the specific functional group in guanine which reacts with mitomycin C, reactions were run between the antibiotic and poly(G) analogs in which guanine was blocked at the N-7 or O-6 position, or lacked the 2-amino group. Binding ratios were affected to a small extent in the two former cases, but binding was significantly decreased in the absence of the 2-amino group. These results indicate that the most likely binding site of mitomycin C in synthetic polyribonucleotides is the 2-amino group of guanine residues.
mesh_terms: D000903:Antibiotics, Antineoplastic; D055598:Chemical Phenomena; D002621:Chemistry; D016685:Mitomycin; D008937:Mitomycins; D011068:Poly G / Q000031:analogs & derivatives*; D011131:Polyribonucleotides; D013329:Structure-Activity Relationship
chemical_list: D000903:Antibiotics, Antineoplastic; D008937:Mitomycins; D011131:Polyribonucleotides; D011068:Poly G; D016685:Mitomycin