Automated enzyme-linked fluorescence immunoassays for antiviral antibodies: a test for anti-HBs.
A solid-phase enzyme-linked fluorescence immunoassay (ELFA) for antiviral antibodies using antigen covalently linked to Escherichia coli beta-galactosidase and a fluorogenic substrate (4-methyl-umbelliferyl-beta-D-galactopyranoside) is described. Antibodies to hepatitis B surface antigen (HBsAg) from test samples were adsorbed to wells of 96-well aminopolystyrene plates precoated with HBsAg. The antibodies were subsequently detected using enzyme-conjugated HBsAg. The measurement of fluorescence was automated. The sensitivity of the test was approximately 50 times higher than that of a commercial radioimmunoassay.
D000914:Antibodies, Viral / Q000032:analysis*; D005453:Fluorescence; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D013050:Spectrometry, Fluorescence; D001616:beta-Galactosidase
D000914:Antibodies, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D001616:beta-Galactosidase