Specific radioimmunoassay of cyclo (His-Pro), a biologically active metabolite of thyrotropin-releasing hormone.
We have developed a radioimmunoassay that can detect 10 pg cyclo (His-Pro) in rat brain perchloric acid extracts. The normal rat brain concentration of cyclo (His-Pro) ranged from 35-61 pmole/brain. The elution profiles of cyclo (His-Pro)-like immunoreactivity and radioactive cyclo (His-Pro) following gel filtration and ion-exchange chromatography were similar.
D000818:Animals; D001923:Brain Chemistry; D003429:Cross Reactions; D005260:Female; D007106:Immune Sera; D008832:Microchemistry; D010456:Peptides, Cyclic / Q000032:analysis*; D010879:Piperazines / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D013973:Thyrotropin-Releasing Hormone / Q000032:analysis
D007106:Immune Sera; D010456:Peptides, Cyclic; D010879:Piperazines; D013973:Thyrotropin-Releasing Hormone; C015924:histidyl-proline diketopiperazine