title: Functional properties of noradrenergic nervous system in rat colonic mucosa: uptake of [3H]norepinephrine.
abstract: The accumulation of exogenous [3H]norepinephrine ([3H]NE) by rat colonic mucosa was studied. Uptake was linear for 10 min and reached a maximum after 90 min. The process was concentration dependent and saturable, having a Km of 1.67 X 10(-6) M and Vmax of 0.57 nmol.g-1.min-1. The inhibitor of specific norepinephrine uptake, desmethylimipramine (DMI), inhibited uptake in a concentration-dependent manner; the maximum inhibition was 81% at 10 microM. Normetanephrine also inhibited uptake at 100 microM. Reserpine, at concentrations ranging from 10(-7) to 10(-5) M, prevented the accumulation of [3H]NE, with maximum inhibition being 47% of control. Accumulation by mucosa obtained from rats sympathectomized with 6-hydroxydopamine was only 33% of control; DMI did not further reduce this uptake. Colonic epithelial cells were isolated and were found to also accumulate [3H]NE, but this accumulation was not affected by DMI. It is concluded that rat colonic mucosa contains noradrenergic neurons capable of accumulating exogenously administered norepinephrine by a specific and high-affinity process. The presence of a functional noradrenergic neural network in close association with the epithelium suggests that this system may play a physiological role in modulating colonic mucosal function.
mesh_terms: D000818:Animals; D003106:Colon / Q000502:physiology*; D003891:Desipramine / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D006892:Hydroxydopamines / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000502:physiology; D008297:Male; D009411:Nerve Endings / Q000502:physiology; D009638:Norepinephrine / Q000378:metabolism*; D016627:Oxidopamine; D051381:Rats; D012110:Reserpine / Q000494:pharmacology; D013564:Sympathetic Nervous System / Q000502:physiology*; D014316:Tritium
chemical_list: D006892:Hydroxydopamines; D014316:Tritium; D012110:Reserpine; D016627:Oxidopamine; D003891:Desipramine; D009638:Norepinephrine