[Immunochemical analysis and protective properties of components of Pseudomonas aeruginosa mucus with different molecular weights].
P. aeruginosa slime has been separated into fractions XM-300 (3 X 10(5) daltons and more), XM-100 (1 X 10(5) = 3 X 10(5) daltons), PM-30 (3 X 10(4) = 1 X 10(5) daltons) and PM-10, (1 X 10(4) = 3 X 10(4) daltons) by ultrafiltration. The high-molecular slime components (3 X 10(4) daltons and more) have been found to be serologically more active than the low-molecular components (1 X 10(4) = 3 X 10(4) daltons). As shown in experiments on mice, both high-molecular toxic and low-molecular nontoxic slime components have protective activity, but the high-molecular components are more active than the low-molecular ones. The slime components stimulate the formation of immunity to homologous and partially heterologous P. aeruginosa strains in mice. Antigenic relationship between the slime components (especially the high-molecular ones) and the corresponding lipopolysaccharides has been noted.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D001427:Bacterial Toxins / Q000032:analysis*; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D008070:Lipopolysaccharides / Q000032:analysis* / Q000276:immunology; D051379:Mice; D008970:Molecular Weight; D011552:Pseudomonas Infections / Q000517:prevention & control; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D014611:Vaccination
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D008070:Lipopolysaccharides
Immunokhimicheskaia analiz i protektivnye svoĭstva komponentov slizi Pseudomonas aeruginosa s raznoĭ molekuliarnoĭ massoĭ.