Effect of aldehydes on polyamine metabolism. I. Method used to determine CO2 produced "in vitro" in enzymatic reactions: its application in evaluation of S-adenosylmethionine decarboxylase (SAMD) activity.
In this paper the Authors describe a new method they have adopted (in studies reported in accompanying papers II and III) for measuring S-adenosylmethionine decarboxylase activity in entire cells and cell fractions. The new method is a general one for recovering and measuring labelled carbon dioxide formed "in vitro" during enzymatic reactions. It presents several advantages when compared to traditional methods.
D012437:Adenosylmethionine Decarboxylase / Q000378:metabolism*; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008722:Methods; D011700:Putrescine / Q000378:metabolism*; D051381:Rats
D002245:Carbon Dioxide; D002262:Carboxy-Lyases; D012437:Adenosylmethionine Decarboxylase; D011700:Putrescine