Role and strain distribution of genes controlling light chains needed for the expression of an intrastrain cross-reactive idiotype.
Several strains of mice were tested for their capacity to provide immunoglobulin L chains required for the expression of the major cross-reactive idiotype (CRIA) associated with p-azophenylarsonate-specific antibodies of strain A mice. To facilitate testing, mice were bred that were homozygous for Igh-Ce and Lyt-2a, 3a, i.e., they possessed genes controlling H chains but not L chains required for expression of the CRIA. Such male mice were mated to females of various strains and their offspring were tested; expression of CRIA indicated the presence in the female parent of genes controlling the appropriate L chains. All females bearing the Lyt-2a, 3b or Lyt-2b, 3b genotype yielded offspring, most of which were CRIA+, whereas all the offspring of females that were Lyt-2a, 3a were CRIA-. The female parents included mice of several strains that are congenic for Lyt-2a, 3a, Lyt-2b, 3b or Lyt-2a, 3b, thus demonstrating very close linkage between the Lyt loci and the expression of CRIA. In addition, doubly congenic strains of mice with the heavy chain allotype of the CRIA+ AL/N strain and the Lyt-2a, 3a genotype on a BALB/c background failed to express CRIA. The data provide further evidence for the similarity of repertoires of L chains in Lyt-3b mice of various strains. When genes were present controlling A/J H chains and L chains of C57BL/6 or BALB/c origin, the quantitative expression of CRIA was only slightly lower than that observed in A/J mice. Mice possessing genes controlling the H or L chains required for CRIA expression, but not both, did not express CRIA but synthesized Ar-specific antibodies which contained low but significant concentrations of the idiotype-associated chain.
D000818:Animals; D000906:Antibodies; D000950:Antigens, Ly / Q000235:genetics; D003429:Cross Reactions; D003433:Crosses, Genetic; D005260:Female; D007130:Immunoglobulin Idiotypes / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D011863:Radioimmunoassay; D010132:p-Azobenzenearsonate / Q000276:immunology
D000906:Antibodies; D000950:Antigens, Ly; D007130:Immunoglobulin Idiotypes; D007147:Immunoglobulin Light Chains; D010132:p-Azobenzenearsonate