Involvement of glycosaminoglycans in detachment of early myeloid precursors from bone-marrow stromal cells.
Fibroblast-like cells were obtained by in vitro cultivation of needle aspirations of human bone-marrow. These cells show a unique composition of coat-associated glycosaminoglycans: 10% chondroitin 4-sulfate, 30% hyaluronic acid and 60% heparan sulfate which were resolved and characterized by electrophoresis, nitrous acid treatment and enzymatic degradation. Chondroitin 4-sulfate is the only glycosaminoglycan detectable on the surface of mature granulocytes, whereas the immature cells do not seem to possess surface glycosaminoglycans. Immature hemopoietic cells can adhere on to marrow-derived fibroblast cells, whereas mature granulocytes cannot. Treatment with mucopolysaccharidases of both mature leukocytes and marrow stromal cells can interfere in these adhesive relationships, suggesting a role of glycosaminoglycans in regulating short-range interactions during hematopoiesis.
D001854:Bone Marrow Cells; D002448:Cell Adhesion / Q000187:drug effects; D002809:Chondroitin Sulfates / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D007938:Leukemia / Q000097:blood; D007962:Leukocytes / Q000378:metabolism*; D011133:Polysaccharide-Lyases / Q000494:pharmacology
D002809:Chondroitin Sulfates; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase; D011133:Polysaccharide-Lyases; C021349:heparitinsulfate lyase