Characterization of phosphorylcholine- (PC) specific IgE-B cells in CBA/N or (CBA/N x BALB/c)F1 male mice.
Surface immunoglobulin isotype of precursors for PC-specific IgE response in CBA/N or NBF1 male mice was studied. Immunization of CBA/N or NBF1 male mice, which had been lethally irradiated and reconstituted with KLH-primed syngeneic spleen cells, with PC-KLH induced the PC-specific IgE, but not the IgM, response. Depletion of mu-bearing cells from KLH-primed spleen cells completely abolished the induction of the anti-PC IgE response. Depletion of T15 idiotype-bearing cells with anti-T15-coated dishes also abrogated the anti-PC IgE response in NBF1 male mice. Mice that were transferred with delta- or epsilon-bearing cell-depleted cell populations did not show the anti-PC IgE response on day 14, but they showed the anti-PC IgE response on day 21. These results indicated that PC-specific IgE-producing cells in CBA/N mice, which were defective in the anti-PC IgM response, were derived from PC-specific mu-bearing cells, and direct precursors bore mu, delta, and epsilon on their surface.
D000818:Animals; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D001665:Binding Sites; D002352:Carrier Proteins / Q000276:immunology; D002794:Choline / Q000031:analogs & derivatives*; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000096:biosynthesis; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M / Q000096:biosynthesis; D007138:Immunoglobulin delta-Chains; D007139:Immunoglobulin epsilon-Chains; D007148:Immunoglobulin mu-Chains; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D010767:Phosphorylcholine / Q000276:immunology*; D011817:Rabbits
D002352:Carrier Proteins; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M; D007138:Immunoglobulin delta-Chains; D007139:Immunoglobulin epsilon-Chains; D007148:Immunoglobulin mu-Chains; D010767:Phosphorylcholine; D007073:Immunoglobulin E; D002794:Choline