Study of the structure of troponin-I by measuring the relative reactivities of lysines with acetic anhydride.
A competitive labeling method that measures the relative reactivity of lysines was used to study the structure of troponin-I. Troponin-I was acetylated free and complexed with troponin-C and troponin-T in the native state with [3H]acetic anhydride. The [3H]troponin-I was combined with [14C]troponin-I that had been acetylated in 6 M guanidine HCl and completely chemically labeled. Peptides containing labeled lysines were isolated following digestion with trypsin and Staphylococcus aureus protease and identified in the published sequence. The 3H/14C ratio of these peptides was used as a measure of the relative reactivity of the lysines. Troponin-I contains 24 lysines; we have identified 23 of these in 16 peptides. When troponin-I is labeled in a native complex, the lysines in the region from residues 40 to 98 are influenced: five become relatively less reactive (40, 65, 70, 78, and 90) and three become relatively more reactive (84, 87), and 98). All of these changes except Lys 70 can be seen when troponin-I binds to troponin-T. Lys 70 is reduced in reactivity when it binds to troponin-C. The lysines that appear to be important in binding of troponin-I to troponin-T are influenced by the binding of Ca2+ to troponin-C in the native troponin complex (in the presence of 2 mM MgCl2), suggesting for the first time that the troponin-IT interaction is affected by Ca2+.
D000085:Acetates; D000089:Acetic Anhydrides; D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium; D008239:Lysine; D009124:Muscle Proteins; D010446:Peptide Fragments / Q000032:analysis; D011485:Protein Binding; D014336:Troponin; D019210:Troponin I
D000085:Acetates; D000089:Acetic Anhydrides; D009124:Muscle Proteins; D010446:Peptide Fragments; D014336:Troponin; D019210:Troponin I; C031800:acetic anhydride; D008239:Lysine; D002118:Calcium