Detection of human, rat and mouse adenosine deaminase by immunochemical and immunomorphologic methods using antiserum to calf enzyme.
A commercial preparation of calf adenosine deaminase (calf ADA) was further purified by affinity chromatography and used for immunization of rabbits. The resulting anti-calf-ADA sera reacted by immunodiffusion with both calf and human ADA, and precipitated about 90% of radiolabeled enzyme isolated from human thymus tissue. Moreover, ADA activity was detected in the pellets formed by immunoprecipitation of unlabeled human enzyme by anti-calf-ADA sera. These antisera were successfully used for the immunomorphologic localization of ADA in human thymus tissue and in lymphoid cell preparations. The anti-calf ADA sera could also be used for the immunofluorescent detection of enzyme in rat and mouse thymocytes. The utilization of anti-calf-ADA serum for immunochemical and immunomorphologic detection of enzyme provides a valuable and sensitive reagent for the identification of ADA-positive cells in humans and several other species.
D000243:Adenosine Deaminase / Q000032:analysis*; D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D002417:Cattle / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D051379:Mice; D008805:Mice, Inbred A; D009700:Nucleoside Deaminases / Q000032:analysis*; D011234:Precipitins; D011817:Rabbits; D051381:Rats; D011916:Rats, Inbred F344; D013950:Thymus Gland / Q000276:immunology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D007106:Immune Sera; D011234:Precipitins; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase