Enhancement of 5-lipoxygenase activity in mastocytoma P-815 cells by n-butyrate treatment.
Cloned mastocytoma P-815, 2-E-6 cells were used to investigate regulation of 5-lipoxygenase activity. 2-E-6 cells had high 5-lipoxygenase activity with slight 12-lipoxygenase activity. The 5-lipoxygenase activity was increased over 5-fold by treatment of the cells with 1 mM n-butyrate for 18 h. the most effective dose range being 0.1-5.0 mM. Treatment with n-butyrate for 18 h was more effective than treatment for 40 h. Addition of n-butyrate to an untreated cell homogenate had no stimulatory effect. The enhancement of 5-lipoxygenase activity by n-butyrate was accompanied by new synthesis of protein(s). 12-Lipoxygenase activity was not increased so much as 5-lipoxygenase activity by the treatment. This is the first report of stimulation of 5-lipoxygenase activity in cultured cells. The different responses of the two lipoxygenases to n-butyrate treatment strongly suggest that 5-lipoxygenase is a different enzyme from 12-lipoxygenase.
D000818:Animals; D001091:Arachidonate Lipoxygenases; D002087:Butyrates / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D002999:Clone Cells; D003513:Cycloheximide / Q000494:pharmacology; D004789:Enzyme Activation; D007700:Kinetics; D008084:Lipoxygenase / Q000378:metabolism*; D012515:Mast-Cell Sarcoma / Q000201:enzymology*; D051379:Mice; D012513:Sarcoma, Experimental / Q000201:enzymology
D002087:Butyrates; D003513:Cycloheximide; D001091:Arachidonate Lipoxygenases; D008084:Lipoxygenase; D002118:Calcium