Functional and binding activity of monoclonal anti-Thy-1 antibodies: evidence for different expression of the two alleles.
Monoclonal anti-Thy-1.1 and anti-Thy-1.2 antisera selected for complement-dependent cytotoxicity have high cytotoxic and binding titers on thymocytes and peripheral T cells of mouse strains bearing the appropriate Thy-1 allele. The effect of both anti-Thy=1.1 and anti-Thy-1.2 monoclonal antisera plus complement on cytoxic T cell effectors is to abrogate their activity. On the functional activity of precursor cytotoxic T cells, monoclonal antisera against the two alleles have different effects: anti-Thy1.2 plus complement removes precursor activity of Thy-1.2-bearing strains, including (Thy-1.1 X Thy-1.2) F heterozygotes, In contrast, six different anti-Thy-1.1 monoclonals, including four of the IgM class and two of the IgG class, failed to remove cytotoxic precursor activity from the splenic T cells of AKR, A. Thy-1.1 or (CBA X AKR) F1 mice. Analysis by florescence-activated cell sorting of in vitro cultured AKR spleen cells shows that Thy-1.1 antigen appears on the cel surface during the five-day culture period.
D000483:Alleles; D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000502:physiology*; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D003165:Complement System Proteins / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D005455:Fluorescent Antibody Technique; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology; D013997:Time Factors
D000906:Antibodies; D000911:Antibodies, Monoclonal; D003165:Complement System Proteins