Calorimetric analysis of sugars and sugar derivatives with aid of an enzyme thermistor.
Enzymic determination of L-ascorbic acid, D-galactose, D-glucose, cellobiose, lactose, and sucrose with an enzyme thermistor is described. The enzyme thermistor is a simple flow-calorimeter designed for routine analysis and measures metabolites by the heat produced in a small column with immobilized enzyme. This instrument may be used for determination of discrete samples as well as for continuous monitoring. As the change of enthalpy produced by the primary reaction is usually large enough to make the measurement sufficiently accurate, simple assay procedures can be designed, an advantage of great importance when the samples are crude and complex.
D001204:Ascorbate Oxidase / Q000378:metabolism; D002151:Calorimetry / Q000379:methods; D002241:Carbohydrates; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005692:Galactose Oxidase / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D043324:beta-Fructofuranosidase; D001616:beta-Galactosidase / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
D002241:Carbohydrates; D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D005692:Galactose Oxidase; D001204:Ascorbate Oxidase; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; D001616:beta-Galactosidase; D043324:beta-Fructofuranosidase