A study of oxygen isotope scrambling in the enzymic and non-enzymic oxidation of linoleic acid.
Lipoxygenases-1 and -2 isolated from soybeans were incubated with linoleic acid in the presence of a mixture of 16O2 and 18O2. The formation of 16O/18O-molecules which is indicative for a head-to head reaction of peroxy radicals was determined and compared with that produced during autoxidation of a linoleic acid emulsion in the presence of ferric ions. Lipoxygenase-1 was much less active in scrambling than lipoxygenase-2 which was comparable to that found in the autoxidation reaction.
D007527:Isoenzymes / Q000378:metabolism; D019787:Linoleic Acid; D008041:Linoleic Acids; D008084:Lipoxygenase / Q000378:metabolism*; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D010103:Oxygen Isotopes; D010944:Plants / Q000201:enzymology; D013025:Glycine max
D007527:Isoenzymes; D008041:Linoleic Acids; D010103:Oxygen Isotopes; D019787:Linoleic Acid; D008084:Lipoxygenase