LPS-induced enhancement of plaque-forming cell response to bromelain-treated syngeneic erythrocytes in mouse peritoneal cell cultures.
The effect of bacterial lipopolysaccharide (LPS) on the development of plaque-forming cells (PFC) against bromelain-treated syngeneic mouse red blood cells (Br-MRBC) was studied in peritoneal cell (PC) cultures. It was found that LPS enhances the development of PFC to Br-MRBC and increases DNA synthesis in PC cultures. The LPS-induced enhancement of PFC to Br-MRBC, however, does not appear to require cell proliferation, since it also occurred in PC cultures pretreated with mitomycin C. In addition, the LPS-induced B lymphocytes blastogenesis is under the control of macrophages, while cell differentiation of precursor B lymphocytes into cells actively producing antibodies against Br-MRBC is regulated by suppressor T lymphocytes.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D001202:Ascitic Fluid / Q000166:cytology; D001963:Bromelains / Q000494:pharmacology*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D006462:Hemolytic Plaque Technique; D008070:Lipopolysaccharides; D051379:Mice; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology
D008070:Lipopolysaccharides; D008937:Mitomycins; D011135:Polysaccharides, Bacterial; D016685:Mitomycin; D001963:Bromelains; D004247:DNA