A rapid and sensitive solid-phase enzyme immunoassay for C-reactive protein.
C-reactive protein (CRP) is an acute-phase reactant the concentration of which increases significantly following tissue injury or inflammation. I report here the development of a rapid and sensitive solid-phase enzyme immunoassay (EIA) for determination of serum CRP. The inclusion of 4% (w/v) polyethylene glycol (PEG) in the diluent buffers made it possible to quantitate CRP within 4 h at room temperature. The minimum detectable amount was about 500 pg CRP/ml. Excellent correlation in determination of serum CRP was found between solid-phase EIA and nephelometric assay, radial immunodiffusion and the qualitative latex agglutination tests.
D000818:Animals; D002097:C-Reactive Protein / Q000032:analysis / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D007841:Latex Fixation Tests; D009391:Nephelometry and Turbidimetry; D011817:Rabbits; D013552:Swine
D002097:C-Reactive Protein