title: Conservation of secondary structure in 5 S ribosomal RNA: a uniform model for eukaryotic, eubacterial, archaebacterial and organelle sequences is energetically favourable.
abstract: The most commonly accepted secondary structure models for 5S RNA differ for molecules of eubacterial origin, where the four-helix model of Fox and Woese is generally cited, and those of eukaryotic origin, where a fifth helix is assumed to exist. We have carefully aligned all available sequences from eukaryotes, eubacteria, chloroplasts, archaebacteria and plant mitochondria. We could thus derive a unified secondary structure model applicable to all 5S RNA sequences known to-date. It contains the five helices already present in the eukaryotic model, extended by additional segments that were not previously assumed to be universally present. One of the helices can be written in two equilibrium forms, which could reflect the existence of a flexible, dynamic structure. For the derivation of the model and the estimation of the free energies we followed a set of rules optimized to predict the tRNA cloverleaf. The stability of the unified model is higher than that of nearly all previously proposed sequence-specific and general models.
mesh_terms: D000818:Animals; D001105:Archaea / Q000032:analysis; D001419:Bacteria / Q000032:analysis; D001483:Base Sequence; D005057:Eukaryotic Cells / Q000032:analysis; D006801:Humans; D008958:Models, Molecular; D010944:Plants / Q000032:analysis; D012335:RNA, Ribosomal; D013045:Species Specificity; D013816:Thermodynamics
chemical_list: D012335:RNA, Ribosomal