[Effect of certain essential amino acids excess on formation of aminoacyl-tRNA against a background of protein deficiency].
Optimal conditions are determined for the rat liver tRNA amino acylation with valine and methionine in norm, under protein deficiency and feeding of valine and methionine excess against a background of protein-free diet. It is shown that the level of the liver tRNA amino acylation with the protein deficiency is considerably higher than in the normal state. The valine and methionine excess against a background of protein-free diet inhibits the intensity of the valyl-tRNA and methionyl-tRNA formation. The cross experiments in a heterosystem show that this inhibition is due to changes in the activity or content of valyl- and methionyl-tRNA-synthetases in the rat liver.
D000818:Animals; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008715:Methionine / Q000378:metabolism*; D008718:Methionine-tRNA Ligase / Q000378:metabolism; D011502:Protein-Energy Malnutrition / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000096:biosynthesis*; D051381:Rats; D014633:Valine / Q000378:metabolism*; D014637:Valine-tRNA Ligase / Q000378:metabolism
D012346:RNA, Transfer, Amino Acyl; D008715:Methionine; D008718:Methionine-tRNA Ligase; D014637:Valine-tRNA Ligase; D014633:Valine
O deĭstvii izbytka otdel'nykh nezamenimykh aminokislot na fone belkovogo golodaniia na obrazovanie aminotsil-tRNK.