[3H]2-deoxyglucose transport by slices of cerebral cortex: apparent dependence upon mitochondrial energy.
The transport of [3H]2-deoxyglucose by brain slices was studied. Cerebral cortex slices were incubated in vitro in the presence of [3H]2-deoxyglucose, or L-[3H]glucose as a marker for diffusion. Transport was defined as the difference between [3H]2DG uptake and L-[3H]glucose uptake. Half-maximal velocity was seen at 2.0 mM 2DG and [3H]2DG transport was not inhibited by 20-fold higher concentrations of L-glucose. Net [3H]2DG transport was unchanged in media deficient in Na+, K+, Mg2+, Ca2+ or Cl-. Uptake was significantly inhibited by 1.0 mM 2,4-DNP and a suggestion of inhibition by azide was seen. These data are consistent with a hypothesis that hexose transport in the brain depends to some extent upon mitochondrial energy.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002412:Cations; D002540:Cerebral Cortex / Q000378:metabolism*; D002794:Choline / Q000494:pharmacology; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D004533:Egtazic Acid / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D002412:Cations; D003837:Deoxy Sugars; D004533:Egtazic Acid; D003847:Deoxyglucose; D002794:Choline