A simple method for the determination of DNA molecular weight distribution.
A method is described for the molecular weight distribution of DNA which is determined from sedimentation-velocity analysis. Knowing the distribution of sedimentation coefficients for a single DNA concentration it is possible to extrapolate such a distribution to infinite dilution of the solute in a simple way. Two versions (using two or three terms of a series) of extrapolating equations are considered and discussed in detail. The sedimentation coefficients distribution calculated from these equations differs only insignificantly with that obtained in a conventional way.
D001412:Bacillus subtilis / Q000032:analysis; D002498:Centrifugation / Q000379:methods; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000032:analysis; D008433:Mathematics; D008970:Molecular Weight; D012505:Sarcina / Q000032:analysis
D004269:DNA, Bacterial