Antitumor activity and hydrogen peroxide release by macrophages elicited by trehalose diesters.
Trehalose diesters (natural 6,6'-trehalose dimycolate from Mycobacterium tuberculosis or synthetic (a 76 carbon atom analogue)), when suspended in water, give stable and well-defined emulsions. These emulsions, injected i.p. in mice significantly limit the growth of P815 syngeneic mastocytoma cells. They elicit macrophages with a cytostatic activity against P815 cells in vitro, strong enough to be expressed at low effector to target ratios (E/T = 1.4) or after a short coincubation period (2 hr). The antitumor potential of these macrophages seems to coincide with their ability to release H2O2 upon pharmacologic triggering. Depressed levels of alkaline phosphodiesterase and beta-galactosidase are proposed as other biochemical markers of cytostatic macrophages.
D000818:Animals; D003311:Cord Factors / Q000494:pharmacology* / Q000627:therapeutic use; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D004655:Emulsions; D006017:Glycolipids / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008262:Macrophage Activation; D008264:Macrophages / Q000145:classification / Q000201:enzymology / Q000378:metabolism; D012515:Mast-Cell Sarcoma / Q000188:drug therapy / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008854:Microscopy, Electron; D013997:Time Factors
D003311:Cord Factors; D004655:Emulsions; D006017:Glycolipids; D006861:Hydrogen Peroxide