Identification of a mitochondrial phosphoprotein in brain synaptic membrane preparations.
A 41,000-dalton phosphoprotein in crude synaptosomal membrane fractions is characterized by its unique divalent and monovalent cation regulation. It is identified by two-dimensional gel electrophoresis as the phosphoprotein whose phosphorylation is enhanced by repetitive electrical stimulation of hippocampal brain slices. After sucrose-gradient ultracentrifugation, this phosphoprotein is found in the mitochondrial subfraction. This suggests that the electrically produced changes in the level of phosphorylation of the 41,000-dalton polypeptide are probably effects on cellular energetics rather than on specialized neural membrane function.
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D008970:Molecular Weight; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*
D010750:Phosphoproteins; D004492:Edetic Acid; D011188:Potassium