Phosphorylation of deoxyguanosine by B and T lymphocytes: evidence against selective trapping of deoxyguanosine by T lymphocytes in purine nucleoside phosphorylase deficiency.
T and B lymphocytes from blood and tonsils, as well as T and B lymphoid cell lines, phosphorylate deoxyguanosine which is probably the major toxic metabolite in purine nucleoside phosphorylase (PNP) deficiency. T and B cells also have similar activities of purine nucleoside phosphorylase. These findings indicate that the selective T cell defect in patients with PNP deficiency is unlikely to be due to a selective 'trapping' of deoxyguanosine by T lymphocytes.
D000243:Adenosine Deaminase / Q000378:metabolism; D001402:B-Lymphocytes / Q000378:metabolism*; D002460:Cell Line; D002648:Child; D003849:Deoxyguanosine / Q000378:metabolism*; D006801:Humans; D014066:Palatine Tonsil / Q000201:enzymology; D010430:Pentosyltransferases / Q000172:deficiency*; D010766:Phosphorylation; D011683:Purine-Nucleoside Phosphorylase / Q000172:deficiency*; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*
D011947:Receptors, Antigen, B-Cell; D010430:Pentosyltransferases; D011683:Purine-Nucleoside Phosphorylase; D000243:Adenosine Deaminase; D003849:Deoxyguanosine