title: Acidic glycosaminoglycans inhibit the uptake of tritiated thymidine by rabbit aortic smooth muscle cells in culture.
abstract: The effect of exogenous glycosaminoglycans on the growth rate of rabbit aortic smooth muscle cells in culture was studied by determinations of cell number, cell protein and the uptake of [3H]thymidine as well as its incorporation into DNA. Glycosaminoglycans did not affect the growth rate of the cells. Expect for heparin, however, all the glycosaminoglycans studied chondroitin 4-sulphate, chondroitin 6-sulphate, dermatan sulphate and hyaluronic acid interfered with the uptake of [3H]thymidine into the cells. So errors may arise if [3H]thymidine-methods alone are used when studying the growth rate of cultured cells in the presence of glycosaminoglycans.
mesh_terms: D000818:Animals; D001011:Aorta; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D003871:Dermatan Sulfate / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000494:pharmacology; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D011817:Rabbits; D013936:Thymidine / Q000378:metabolism*
chemical_list: D006025:Glycosaminoglycans; D009124:Muscle Proteins; D003871:Dermatan Sulfate; D006820:Hyaluronic Acid; D006493:Heparin; D002809:Chondroitin Sulfates; D004247:DNA; D013936:Thymidine