[The analysis of localization and function of proteins in eukaryotic ribosomes by means of antibodies (author's transl)].
Due to their high specificity antibodies reveal possibilities for a detailed analysis of structure and function of eukaryotic ribosomes. By means of specific antibodies against single ribosomal proteins we could (1) localize immunoelectron microscopically certain proteins at the surface of ribosomes and (2) determine their role within protein biosynthesis by the binding of the initiation factor eIF-2 to the small ribosomal subunit. The combination of the results of both methods provides evidence of the localization of the peptidyl-tRNA binding site in the head area of the small subunit of ribosomes.
D000818:Animals; D000937:Antigen-Antibody Reactions; D015852:Eukaryotic Initiation Factor-2; D007118:Immunoassay; D008099:Liver / Q000648:ultrastructure; D008854:Microscopy, Electron; D010448:Peptide Initiation Factors / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl; D051381:Rats; D012269:Ribosomal Proteins / Q000032:analysis* / Q000502:physiology; D012270:Ribosomes / Q000032:analysis* / Q000378:metabolism
D015852:Eukaryotic Initiation Factor-2; D010448:Peptide Initiation Factors; D010455:Peptides; D011506:Proteins; D012346:RNA, Transfer, Amino Acyl; D012269:Ribosomal Proteins; C019037:tRNA, peptidyl-; D012343:RNA, Transfer
Analyse der Lokalisation und Funktion der Proteine von Eukaryontenribosomen mit Hilfe von Antikörpern.