Sperm-immobilizing monoclonal antibody to human seminal plasma antigens.
Rat spleen cells immunized to human azoospermic semen (a mixture of seminal plasma components) and mouse myeloma cells (P3/X63 Ag8U1; P3U1) (Marguilies et al., 1976) were successfully fused with polyethylene glycol (PEG 1500) and 19 of 89 fused cell cultures were found to produce sperm-immobilizing antibody. The cells that produced antibody indicating the highest sperm-immobilizing activity were distributed into wells for further recloning and 10 clones producing sperm-immobilizing antibody were established. The clone (1C4) producing the highest antibody titre was found to produce a large amount of IgG in culture supernatants and to contain a mixture of rat and mouse chromosomes. It was proved by immunodiffusion test that the monoclonal antibody was produced to the human seminal plasma antigen No. 7 which is common to human milk protein. Using this hybridoma which produced a large amount of monoclonal sperm-immobilizing antibody, a new method could be developed for purifying human seminal plasma antigen by immunoaffinity chromatography with bound antibody from the hybridoma.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D002460:Cell Line; D002999:Clone Cells; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology; D005779:Immunodiffusion; D008297:Male; D051381:Rats; D012661:Semen / Q000276:immunology*; D013081:Sperm Motility
D000906:Antibodies; D000941:Antigens