title: In vitro effects of acephate on carbonic anhydrase activity in the blood and gills of rainbow trout, Salmo gairdneri.
abstract: Acephate, a water-soluble organophosphate pesticide used to control terrestrial insect pests, may enter aquatic ecosystems in the course of its use and adversely affect fish populations. The in vitro effects of this insecticide on gill and red blood cell (RBC) carbonic anyhdrase (CA) activity in rainbow trout were investigated over a range of 100 mg/1 (0.55 mM) to 50,000 mg/l (273 mM) to assess the manner in which acephate might affect respiratory capacity in exposed fish. Concentrations required to produce 50% inhibition of CA activity in the gill and RBC preparations were 38,000 mg/l (207 mM) and 8,900 mg/l (48 mM) respectively. The toxic action of acephate may be related to inhibition of CA activity in the blood and gills with resultant disturbances of respiratory capacity and salt balance.
mesh_terms: D000818:Animals; D002257:Carbonic Anhydrase Inhibitors; D002256:Carbonic Anhydrases / Q000097:blood; D005618:Fresh Water / Q000032:analysis; D005880:Gills / Q000201:enzymology; D007306:Insecticides / Q000633:toxicity*; D007668:Kidney / Q000201:enzymology; D009946:Organothiophosphorus Compounds / Q000633:toxicity*; D010100:Oxygen / Q000032:analysis; D063088:Phosphoramides; D012487:Salmonidae / Q000378:metabolism*; D014337:Trout / Q000097:blood / Q000378:metabolism*
chemical_list: D002257:Carbonic Anhydrase Inhibitors; D007306:Insecticides; D009946:Organothiophosphorus Compounds; D063088:Phosphoramides; C001969:acephate; D002256:Carbonic Anhydrases; D010100:Oxygen