Effect of cycloheximide, beta-D-xylosides and beta-D-galactosides on heparin biosynthesis in mouse mastocytoma.
Heparin biosynthesis has been investigated with mouse mastocytoma in vitro. Minced tumour tissue catalysed the incorporation of [35S]sulphate and [3H]glucosamine into heparin and to a smaller extent into chondroitin sulphate. Addition of cycloheximide caused an inhibition (greater than 80%) of incorporation of each labelled precursor into both polysaccharides. Addition of benzyl beta-D-xyloside relieved the inhibition of incorporation into chondroitin sulphate and restored it to more than threefold that of the control incubation. The effect of beta-D-xyloside on incorporation into heparin was less marked although a consistent small increase of incorporation into this polysaccharide was observed. beta-D-Xyloside did, however, cause a marked incorporation of 35S and 3H labels into material of low molecular weight, which appeared to comprise heparin-like fragments. It is proposed that these fragments arise through a breakdown of the usual process of heparin biosynthesis.
D000818:Animals; D002809:Chondroitin Sulfates / Q000096:biosynthesis; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003513:Cycloheximide / Q000494:pharmacology*; D005697:Galactosides / Q000494:pharmacology*; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006027:Glycosides / Q000494:pharmacology*; D006493:Heparin / Q000096:biosynthesis*; D066298:In Vitro Techniques; D012515:Mast-Cell Sarcoma / Q000378:metabolism*; D051379:Mice; D014994:Xylose / Q000031:analogs & derivatives / Q000494:pharmacology
D005697:Galactosides; D006025:Glycosaminoglycans; D006027:Glycosides; C010807:xylosides; D006493:Heparin; D002809:Chondroitin Sulfates; D003513:Cycloheximide; D014994:Xylose