title: Identification of Theileria infections in the salivary glands of Hyalomma anatolicum anatoli cum and Rhipicephalus appendiculatus using isoenzyme electrophoresis.
abstract: Lysates prepared from the salivary glands of uninfected Hyalomma anatolicum anatolicum and Rhipicephalus appendiculatus and from ticks of these species infected with 2 strains of Theileria annulata and 1 strain of T. parva respectively have been examined for enzyme polymorphism using thin layer starch gel electrophoresis. Representative enzymes from 3 of the major metabolic pathways, the tricarboxylic acid cycle, the glycolytic pathway and the pentose phosphate pathway were investigated. Only 1 enzyme, glucose phosphate isomerase, showed differences between the host cell activity and the parasite activity and also inter- and intraspecific differences between the parasites. This technique represents a useful complement to the present histochemical methods for identification of Theileria infections in the salivary glands of ticks.
mesh_terms: D000818:Animals; D016782:Apicomplexa / Q000145:classification* / Q000201:enzymology; D002417:Cattle; D004592:Electrophoresis, Starch Gel; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis*; D007527:Isoenzymes / Q000032:analysis*; D012469:Salivary Glands / Q000469:parasitology; D013801:Theileriasis / Q000469:parasitology*; D013987:Ticks / Q000469:parasitology*
chemical_list: D007527:Isoenzymes; D005956:Glucose-6-Phosphate Isomerase