A diagnostic skin test for Onchocerca volvulus infection.
Onchocerca supernatant (OS) was prepared by a technique permitting live microfilariae to migrate from nodule tissue through agar gel into sterile Hanks balanced salt/Penicillin-Streptomycin solution where they metabolized. The OS, after dialysis, was passed through Seitz viral filter and either concentrated or lyophilized. Using rabbit antiserum in immunodiffusion and immunoelectrophoresis tests, microfilariae proteins and also human protein were detected in out OS. No common antigens were found between this and somatic extracts of Loa loa, O. gutturosa, O. volvulus, L. carinii, D. immittis and A. lumbricoides. 125I labelled OS was purified by passage through protein A column and then through immunosorbent column of horse anti-human serum linked to CNB-activated sepharose 4B. Autoradiography, after sodium dodecyl sulphate polyacylamide slab gel eletrophoresis of purified OS, showed 10 protein bands in the molecular range 10,000 to 125,000. Skin prick tests with OS, shown not to be contaminated with Hepatitis B antigens, elicited immediate hypersensitivity reaction. Using our criteria, positive reactions were seen in 81% of proven onchocerca cases and only occasionally in Loasis 4.5%, ascaridiasis 13.5% or healthy controls 2.4%. The poor skin reactivity to OS in loasis was not due to immunosuppression as these patients, when also infested with ascaris, reacted just as well as onchocerca patients with ascaris to skin prick test using somatic extracts of ascaris.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000941:Antigens / Q000276:immunology; D001196:Ascariasis / Q000276:immunology; D001200:Ascaris / Q000276:immunology; D002648:Child; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008118:Loiasis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009854:Onchocerca / Q000276:immunology*; D009855:Onchocerciasis / Q000175:diagnosis* / Q000276:immunology; D012882:Skin Tests
D000941:Antigens