title: The monitoring of antigen levels during inactivated poliovirus vaccine production: evaluation of filtration techniques.
abstract: The use of ELISA to estimate poliovirus antigen concentration has permitted an evaluation of the methodology used in vaccine production and allowed exploration of less wasteful filtration-techniques. The replacement of Seitz-EKS-1B filtration with either Seitz-Supra-EKS or Pall-filtration in the preparation of the vaccine could make a large saving in the total antigen yield, but the results in the safety test excluded their possible use in our process as it stands at the moment.
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis*; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration; D005779:Immunodiffusion; D007668:Kidney; D011054:Poliovirus Vaccine, Inactivated / Q000032:analysis* / Q000592:standards
chemical_list: D000941:Antigens; D011054:Poliovirus Vaccine, Inactivated