A loss of GABAergic hippocampus innervation in rats with cobalt-induced epilepsy demonstrated by Wolman's fluorescence method.
The topohistochemistry of gamma-aminobutyric acid (GABA) was studied in the hippocampus of rats with epileptiform activity provoked by cortical implantation of cobalt-agar pellets and in control rats with implanted agar-pellets. Using the GABA specific fluorochroming procedure of Wolman (1971) a distinct decrease of GABA fluorescence was found especially in the pyramidal and granular layer, but only in the animals with a typical epileptiform activity of the EEG. The results support the hypothesis that there is a lack of GABAergic inhibition in epileptic individuals, but the anticonvulsive effect of GABA depends on structural and functional relations in the specific type of seizure.
D000818:Animals; D003035:Cobalt; D004195:Disease Models, Animal; D004569:Electroencephalography; D004827:Epilepsy / Q000503:physiopathology; D006624:Hippocampus / Q000032:analysis*; D006651:Histocytochemistry / Q000379:methods; D008297:Male; D008856:Microscopy, Fluorescence; D051381:Rats; D011919:Rats, Inbred Strains; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D003035:Cobalt; D005680:gamma-Aminobutyric Acid