Urinary free and conjugated 5-S-cysteinyldopa in normal subjects and in patients with melanoma.
We measured free and total 5-S-cysteinyldopa excretion in 24-h urine specimens from nine patients with malignant melanoma and forty-five controls, and found that the levels of 5-S-cysteinyldopa excretion (measured by a fluorometric method) were greatly increased in the melanoma patients. The conjugates were hydrolysed by enzymatic treatment with beta-glucuronidase/arylsulphatase, and we found that the percentage of conjugated 5-S-cysteinyldopa was higher in urines with a low content of free 5-S-cysteinyldopa than in those with a higher concentration.
D000328:Adult; D000368:Aged; D003548:Cysteinyldopa / Q000378:metabolism / Q000652:urine*; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives*; D005260:Female; D005470:Fluorometry; D006801:Humans; D008297:Male; D008545:Melanoma / Q000378:metabolism / Q000652:urine*; D008875:Middle Aged
D003548:Cysteinyldopa; D004295:Dihydroxyphenylalanine