DNA isolated from Mycobacterium leprae: genome size, base ratio, and homology with other related bacteria as determined by optical DNA-DNA reassociation.
DNA derived from Mycobacterium leprae (grown in armadillos) was isolated, purified, and analyzed spectrophotometrically. The genome size and the guanine-plus-cytosine content of M. leprae were 1.3 x 10(9) and 55.8%, respectively. Among selected strains of mycobacterial, nocardial, and corynebacterial species, Corynebacterium sp. 2628 LB, isolated from a human leprosy patient, showed the highest DNA homology with M. leprae. Of the DNAs derived from mycobacteria, those of M. tuberculosis and M. scrofulaceum showed a comparatively high reassociation with the DNMA of M. liprae.
D001482:Base Composition; D001483:Base Sequence; D003352:Corynebacterium / Q000235:genetics; D003596:Cytosine / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis*; D006147:Guanine / Q000032:analysis; D009161:Mycobacterium / Q000235:genetics*; D009166:Mycobacterium leprae / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000235:genetics; D009695:Nucleic Acid Renaturation
D004269:DNA, Bacterial; D006147:Guanine; D003596:Cytosine