Dimethylnitrosamine demethylase activity in fetal, suckling, and maternal mouse liver and its transplacental and transmammary induction by polychlorinated biphenyls.
Oxidative demethylation of dimethylnitrosamine (DMN) was detectable with liver homogenate preparations from fetal mice (noninbred Swiss) by day 16 of gestation; this activity increased to adult levels by postnatal day 7. Treatment of pregnant mice with a mixture of polychlorinated biphenyls (PCB's), 500 mg Aroclor 1254/kg, on the last day of gestation caused a large elevation in DMN demethylase activity in the livers of their sucklings, relative to the normal ontogenic increase, reaching a maximum at postnatal day 21. Foster nursing experiments showed that this inductive effect was due primarily to lactational delivery of the PCB]s. DMN demethylase activity in the maternal livers was significantly lower than normal during pregnancy. The mothers' livers were also less responsive to DMN demethylase induction by PCB's during the last 5 days of gestation in comparison with the response earlier in pregnancy or just after delivery. In the livers of the mothers, induced activity resulting from treatment with PCB's on day 19 of gestation reached a maximum on postnatal days 4-7 and declined to normal levels by the time of weaning.
D000818:Animals; D000833:Animals, Suckling; D001140:Aroclors / Q000378:metabolism*; D019392:Cytochrome P-450 CYP2E1; D004128:Dimethylnitrosamine / Q000096:biosynthesis / Q000378:metabolism; D004790:Enzyme Induction; D005260:Female; D005333:Fetus / Q000187:drug effects / Q000201:enzymology; D005865:Gestational Age; D007774:Lactation; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008431:Maternal-Fetal Exchange; D051379:Mice; D010089:Oxidoreductases, N-Demethylating / Q000096:biosynthesis* / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D011247:Pregnancy
D001140:Aroclors; D011078:Polychlorinated Biphenyls; D019392:Cytochrome P-450 CYP2E1; D010089:Oxidoreductases, N-Demethylating; D004128:Dimethylnitrosamine