title: A mutation in the RNA polymerase beta' subunit causing depressed ribosomal RNA synthesis in Escherichia coli.
abstract: Macromolecular synthesis in an Escherichia coli mutant with a temperature-sensitive beta' subunit of RNA polymerase was analysed. At the non-permissive temperature ribosomal RNA synthesis is strongly reduced while messenger RNA synthesis is affected to only a slightly extent. The overall protein synthesis is only slightly affected. We conclude that the beta' subunit is involved in promoter recognition and plays a role in transcriptional selectivity.
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004792:Enzyme Precursors / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D009876:Operon; D010641:Phenotype; D012333:RNA, Messenger / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D004792:Enzyme Precursors; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012321:DNA-Directed RNA Polymerases; D001616:beta-Galactosidase