Comparison of the cytotoxicity of 5-thio-D-glucose and misonidazole on hypoxic cells in vitro.
The cytotoxicity of 5-thio-D-glucose (5-TG) and misonidazole (MISO) on hypoxic cells in vitro was compared, and the combined effect of these two compounds was investigated. The cytotoxicity of 5-TG on hypoxic SCK mammary carcinoma cells of mice was markedly greater than that of MISO. However, MISO was more toxic than was 5-TG to P815-X2 mastocytoma cells of mice. The elevation of temperature from 37.5 degrees C to 40.0 degrees C during incubation with drugs greatly enhanced the cell death. The thermal enhancement of cytotoxicity of 5-TG to mastocytoma cells appeared to be greater than that of MISO. Preliminary studies indicated that the combined effect of 5-TG and MISO is additive, suggesting the intracellular targets or cytocidal mechanisms of these two compounds may be different.
D000230:Adenocarcinoma / Q000188:drug therapy; D000818:Animals; D000970:Antineoplastic Agents; D002470:Cell Survival / Q000187:drug effects; D005947:Glucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D000860:Hypoxia / Q000503:physiopathology; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy; D012515:Mast-Cell Sarcoma / Q000188:drug therapy; D051379:Mice; D008920:Misonidazole / Q000494:pharmacology*; D009593:Nitroimidazoles / Q000494:pharmacology*; D012513:Sarcoma, Experimental / Q000188:drug therapy
D000970:Antineoplastic Agents; D009593:Nitroimidazoles; C003002:5-thio-D-glucose; D008920:Misonidazole; D005947:Glucose