title: Isolation and characterization of thioredoxin from the cyanobacterium, Anabaena sp.
abstract: Thioredoxin from Anabaena sp. has been purified 800-fold with an assay based on the reduction of insulin disulfides by NADPH and the heterologous calf thymus thioredoxin reductase. The final material was homogeneous on polyacrylamide gel electrophoresis and had a molecular weight of 12,000; the NH2-terminal residue was serine and the COOH-terminal was leucine. Anabaena thioredoxin-(SH)2 is a hydrogen donor for the adenosylcobalamin-dependent anabaena ribonucleotide reductase and is equally active with the iron-containing ribonucleotide reductase from Escherichia coli. Anabaena thioredoxin-S2 is a good substrate for E. coli thioredoxin reductase. We have compared the structure of Anabaena and E. coli thioredoxins. Clear structural differences between the proteins, compatible with the large evolutionary distance between the organisms, were seen with respect to total amino acid composition, isoelectric point, tryptic peptide maps, and a low immunochemical cross-reactivity. However, both thioredoxins contain a single oxidation-reduction active disulfide bridge with the amino acid sequence: Cys-Gly-Pro-Cys-Lys. The tryptophan fluorescence emission of Anabaena thioredoxin-S2 increases more than 3-fold on reduction to thioredoxin-(SH)2. This behavior is identical with that of E. coli thioredoxin, suggesting a very similar overall folding of homologous molecules.
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D005075:Biological Evolution; D000458:Cyanobacteria / Q000378:metabolism*; D007700:Kinetics; D011487:Protein Conformation; D013045:Species Specificity; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism; D013879:Thioredoxins / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D013879:Thioredoxins; D013880:Thioredoxin-Disulfide Reductase