[Production of a mastocyte growth factor in lymphocyte cultures stimulated by a mitogen or by allogenic cells].
Supernatants from mouse spleen leukocytes stimulated by concanavalin A or from a secondary MLC between donor and recipient of a skin allograft can induce in bone marrow cells an exponential growth of mononuclear cells with cytoplasmic basophilic granules. These cells can be maintained in liquid suspension culture for up to 6 months. They lack the properties of T or b lymphocytes, monocytes or macrophages. They have the morphological features of mast cells or basophils; the cytoplasmic granules are metachromatic for toluidine blue, alcian blue-positive at pH 0.3 and contain histamine. In addition, these cells bind monoclonal mouse IgE. They may be considered as cells of the basophil-mast cell lineage.
D000818:Animals; D001853:Bone Marrow / Q000276:immunology; D002448:Cell Adhesion; D002478:Cells, Cultured; D003208:Concanavalin A; D006133:Growth Substances / Q000096:biosynthesis*; D006632:Histamine / Q000032:analysis; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D010587:Phagocytosis; D016038:Skin Transplantation; D014184:Transplantation, Homologous
D006133:Growth Substances; D003208:Concanavalin A; D006632:Histamine
Production d'un facteur de croissance des mastocytes dans les cultures lymphocytaires stimulées par un mitogène ou des cellules allogéniques.