Enhanced adoptive transfer of immunity to Listeria monocytogenes after in vitro culture of murine spleen cells with concanavalin A.
In vitro incubation of spleen cells with T-cell mitogens has been shown to augment cytotoxic and cytolytic T-cell function. The plant lectins Concanavalin A and phytohemagglutinin were employed in a similar fashion to investigate their abilities to enhance cell-mediated immunity to the microbial pathogen Listeria monocytogenes. Spleen cells from immune mice were incubated in vitro with Concanavalin A or phytohemagglutinin before passive transfer into normal recipients. Results indicated a 10- to 100-fold enhancement in the ability of these cells stimulated in vitro to transfer antilisterial resistance, as assayed by changes in 50% lethal dose values and enumeration of splenic bacterial proliferation.
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D005260:Female; D007116:Immunization, Passive; D007928:Lethal Dose 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D008089:Listeria monocytogenes / Q000254:growth & development; D008088:Listeriosis / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010835:Phytohemagglutinins / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000382:microbiology; D013601:T-Lymphocytes / Q000276:immunology*
D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D003208:Concanavalin A