title: Studies on beta-phenylethylamine deamination by human placental monoamine oxidase.
abstract: Kinetical properties of human placental monoamine oxidase (MAO) were investigated in studies on inhibitors and mixed substrates. MAO activity was determined by a radioisotopic assay. Lineweaver-Burk plots were linear at higher and lower concentrations of PEA, whereas at intermediate substrate concentrations, a downward curving plot was obtained. The Km values of the low- and high-affinity sites for PEA deamination were estimated. Studies with mixed substrates showed that 5-HT was a competitive inhibitor and tyramine a mixed-type inhibitor of deamination at high concentrations of PEA, whereas both were non-competitive inhibitors at lower concentrations of PEA. After pre-incubation of human placental mitochondrial preparations with deprenyl, Lineweaver-Burk plots were completely linear, and the Km value was the same as that obtained at low concentrations of PEA in the absence of deprenyl. Tyramine and 5-HT were competitive inhibitors of PEA deamination by deprenyl-treated MAO. From these results it is concluded that there are two kinds of MAO with high- and low-affinity sites for PEA in mitochondria of human placenta, corresponding to type B and A Mao, and that tyramine, 5-HT and PEA share a substrate-binding site on type A Mao, while tyramine and 5-HT bind to a site on type B MAO that is different from the PEA binding site.
mesh_terms: D003641:Deamination; D005260:Female; D006801:Humans; D007700:Kinetics; D008995:Monoamine Oxidase / Q000378:metabolism*; D010627:Phenethylamines / Q000378:metabolism*; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D012642:Selegiline / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology; D014439:Tyramine / Q000494:pharmacology
chemical_list: D010627:Phenethylamines; D012642:Selegiline; D012701:Serotonin; D008995:Monoamine Oxidase; D014439:Tyramine