title: A mutant of Pseudomonas aeruginosa deficient in an ATP-dependent deoxyribonuclease.
abstract: A mutant of Pseudomonas aeruginosa PAO1 originally isolated on the basis of its sensitivity to methyl methanesulphonate was found to be (i) sensitive to u.v.- and gamma-irradiation, (ii)deficient in recombination as assayed by transduction and conjugation and (iii) deficient in an ATP-dependent deoxyribonuclease activity. Its marker (mms-13) is cotransducible with argB and pyrE which are mapped at approximately 22 min on the P. aeruginosa chromosome.
mesh_terms: D000255:Adenosine Triphosphate; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial; D003851:Deoxyribonucleases / Q000172:deficiency*; D005819:Genetic Markers; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D011995:Recombination, Genetic; D014161:Transduction, Genetic
chemical_list: D004269:DNA, Bacterial; D005819:Genetic Markers; D000255:Adenosine Triphosphate; D008741:Methyl Methanesulfonate; D003851:Deoxyribonucleases