Influence of glycosaminoglycans on endogenous DNA synthesis in isolated normal and cancer cell nuclei. Differential effect of heparin.
The influence of exogenously-added glycosaminoglycans and glycoproteins on DNA synthesis in isolated nuclei, from normal and malignant tissues, was investigated. Heparin stimulated DNA synthesis in normal cell nuclei at concentrations (heparin/DNA (w/w) less than 0.9) which inhibited DNA synthesis in tumor cell nuclei. At higher concentrations (heparin/DNA (w/w greater than 0.9) heparin inhibited DNA synthesis in both normal and tumor cell nuclei. The chondroitin-4 and 6-sulfates, heparin sulfate, cartilage proteoglycan, N-desulfated heparin, and glycophorin caused inhibition of DNA synthesis at all concentrations tested and in all nuclei examined. Hyaluronic acid, dermatan sulfate, keratan sulfate, alpha 1-acid glycoprotein and fetuin had no significant influence on DNA synthesis in isolated nuclei.
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects; D002809:Chondroitin Sulfates / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D006025:Glycosaminoglycans / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D009374:Neoplasms, Experimental; D051381:Rats
D004273:DNA, Neoplasm; D006025:Glycosaminoglycans; D006820:Hyaluronic Acid; D006493:Heparin; D002809:Chondroitin Sulfates; D004247:DNA; D008274:Magnesium