title: Morphology of sulfur granules produced by Pseudomonas aeruginosa in cows.
abstract: A sulfur granule produced by Pseudomonas aeruginosa in three cows was studied light- and electron-microscopically. It consisted of clumps of basophilic bacteria and eosinophilic clubs. The clubs radiated from the periphery outward. The bacteria were stained dark red by both PAS and MacCallum-Goodpasture staining. Electron-microscopically, the sulfur granule consisted of electron-dense amorphous material and bacteria. The intact bacterium was about 0.45 micrometers in diameter and had an electron-dense cell wall 15 nm in width. Beneath the cell wall, moderately electron-dense fine granular material was present. The center of the bacterium was electron lucent. The club was electron-dense amorphous material. Degenerative bacteria and pilus-like structures were often seen in it. In one case, clusters of bacteriophages were present in and near the degenerative bacteria. Their head was hexagonal and 40 nm in diameter.
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology* / Q000473:pathology; D005260:Female; D006099:Granuloma / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D008854:Microscopy, Electron; D011552:Pseudomonas Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D011550:Pseudomonas aeruginosa / Q000648:ultrastructure*; D013455:Sulfur / Q000032:analysis*
chemical_list: D013455:Sulfur