Multivalent hybrid antibody with double specificity as a tool for locating cell surface antigens by electron microscopy.
Multivalent hybrid antibody complexes with dual specificity were prepared by combining rabbit antibody with anti-mouse immunoglobulin (mIg) and anti-peroxidase (HRP) specificity using protein A of Staphylococcus aureus. The presence of two antibody molecules with anti-mIg and anti-HRP specificity in a single molecule of hybrid complex was demonstrated by their abilities to produce hemagglutination with both HRP-coated and mIg-coated sheep red cells, to give a reaction of complete identity with mIg and HRP and to allow mIg bearing lymphocytes to form rosettes with HRP-coated sheep red blood cells. Electron microscopy of mouse lymphocytes and thymocytes (previously coated with mIg anti-Thy-1 antibody) treated with hybrid antibody complex and HRP showed strong and specific staining of the cell membrane of both cell types. The hybrid antibody complex containing anti-HRP antibody is a valuable reagent for determining various antigenic markers on cell membranes by electron microscopy.
D000818:Animals; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000032:analysis*; D006384:Hemagglutination; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D007158:Immunologic Techniques; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008854:Microscopy, Electron; D011817:Rabbits; D012397:Rosette Formation; D013205:Staphylococcal Protein A / Q000276:immunology
D000954:Antigens, Surface; D013205:Staphylococcal Protein A