[Differential effect of tunicamycin on glycosylation of cell surface glycoproteins from chick fibroblasts during embryo development (author's transl)].
The antibiotic tunicamycin inhibits glycosylation of proteins as demonstrated by the inhibition of the incorporation of 14C glucosamine glycoproteins of chick embryo fibroblasts at two stages of development. Fibroblasts from 8-day embryos are more sensitive to TM treatment than 16-day embryos. This differential effect is directly related to the level of N- and O-glycosidically linked glycoproteins. This ratio decreased within development. Moreover, a parallelism is observed between inhibition of cell surface glycoprotein glycosylation by TM and adhesion properties of treated cells, suggesting that N- glycosidically linked glycoproteins might be involved in cell adhesivity.
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002642:Chick Embryo; D005347:Fibroblasts / Q000378:metabolism; D005944:Glucosamine / Q000031:analogs & derivatives*; D006023:Glycoproteins / Q000096:biosynthesis*; D008565:Membrane Proteins / Q000378:metabolism*; D013997:Time Factors; D014415:Tunicamycin / Q000494:pharmacology*
D006023:Glycoproteins; D008565:Membrane Proteins; D014415:Tunicamycin; D005944:Glucosamine
Effets différentiels de la tunicamycine sur la glycosylation des glycoprotéines de surface des cellules embryonnaires à différentes étapes du développement.