G-CSF: its relationship to leukemia differentiation-inducing activity and other hemopoietic regulators.
The murine myelomonocytic leukemia WEHI-3B exists as differentiation-inducible (D+) and noninducible (D-) cell lines. Both lines produce a CSF species that stimulates exclusively the formation of neutrophil granulocyte colonies. This G-CSF copurifies with a mast cell growth factor but can be separated from M- and GM-CSF. NZB bone marrow is unresponsive to G-CSF stimulation. WEHI-3B D+ cells can induced to terminal granulocyte differentiation by a factor present in murine and human postendotoxin serum that is different from G-CSF present in WEHI-3B D+ or D- CM since the latter has little or no leukemia differentiation-inducing activity. Endotoxin treatment of C. parvum primed mice leads to simultaneous induction of serum activities with selective action on myeloid leukemic cells, a serum differentiation inducing activity and a leukemic colony inhibitory activity. These factors act synergistically to block leukemic stem cell self-renewal. The results suggest that a variety of inducible factors may have potent and selective antileukemic activity.
D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000096:biosynthesis / Q000494:pharmacology*; D004731:Endotoxins / Q000494:pharmacology; D005260:Female; D006098:Granulocytes; D006133:Growth Substances / Q000096:biosynthesis; D006410:Hematopoiesis / Q000187:drug effects*; D007376:Interleukin-2 / Q000096:biosynthesis; D007377:Interleukin-3; D007739:L Cells; D007951:Leukemia, Myeloid / Q000473:pathology*; D008297:Male; D008407:Mast Cells; D051379:Mice; D008807:Mice, Inbred BALB C
D003115:Colony-Stimulating Factors; D004731:Endotoxins; D006133:Growth Substances; D007376:Interleukin-2; D007377:Interleukin-3