High-resolution autoradiographic study on the presence of chromatin structures within interchromatin granule conglomerations.
In Ehrlich ascites tumour cell nuclei ledakrin (1-nitro-9/3'-dimethylpropyloamine/acridine-2 HCl) induces condensation and margination of chromatin, compaction and segregation of nucleolar components and conglomeration of chromatin granule clusters. Localization of DNA in nuclei of the cells labelled with 3H-thymidine and subsequently treated with ledakrin is studied using high-resolution autoradiography. Besides strong labelling of condensed chromatin some radioactivity is associated with IG conglomerations. Possible role of chromatin fibrils in the formation of conglomerations of IG is discussed.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000032:analysis* / Q000187:drug effects; D002843:Chromatin / Q000032:analysis* / Q000187:drug effects / Q000648:ultrastructure; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009564:Nitracrine / Q000494:pharmacology
D002843:Chromatin; D009564:Nitracrine