title: [The use of alkaline protease from Bacillus subtilis, strain DY, for the hydrolysis of amino acid and peptide esters (author's transl)].
abstract: We have studied the hydrolysis of methyl-, ethyl- and benzylesters of amino acids and peptides using alkaline protease from B. subtilis, strain DY. The hydrolysis with this enzyme gives high yields (usually over 90%) with high optical purity of the products. The proposed method is considerably better than the alkaline saponification or the hydrolysis of these esters with alpha-chymotrypsin. The method is not applicable in the case of substrates with free amino groups as well as with substrates which are insoluble under the conditions of the reaction.
mesh_terms: D000596:Amino Acids; D001412:Bacillus subtilis / Q000201:enzymology*; D010450:Endopeptidases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010455:Peptides; D012697:Serine Endopeptidases; D013379:Substrate Specificity
chemical_list: D000596:Amino Acids; D010455:Peptides; D010450:Endopeptidases; D012697:Serine Endopeptidases; C032923:microbial serine proteinases
vernacular_title: Verwendung von alkalischer Protease des Bacillus-subtilis-Stammes DY zur Hydrolyse von Aminosäure- und Peptid-Estern.