title: Location and release of TRH and 5-HT from amphibian skin.
abstract: The occurrence and release of thyrotrophin-releasing hormone (TRH) and 5-hydroxytryptamine (5-HT) from amphibian skin have been described by previous investigators. In the present study, the precise location and site of release of TRH and 5-HT from the skin of Rana pipiens and Xenopus laevis have been examined using a combination of procedures including immunohistochemistry, HPLC, and radioimmunoassay. The results indicate that TRH is located specifically within the dermal glands of these species, and that both TRH and 5-HT are discharged from these glands following adrenergic stimulation. The origin and functional significance of these substances in amphibian skin granular glands are discussed.
mesh_terms: D000818:Animals; D005088:Exocrine Glands / Q000378:metabolism; D008854:Microscopy, Electron; D009924:Organ Culture Techniques; D011894:Rana pipiens / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*; D012867:Skin / Q000166:cytology / Q000378:metabolism; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism*; D014982:Xenopus laevis / Q000378:metabolism*
chemical_list: D012701:Serotonin; D013973:Thyrotropin-Releasing Hormone