[Mechanism of action of a triphenylethylene type antiestrogen on growth of the human breast cancer cell line. MCF-7 in culture].
The antiestrogen of the triphenylethylene type, Taxomifen (Tx), exerts its inhibitory action on cell multiplication of the human breast cancer cell line MCF-7 in the complete absence of estrogens in the culture medium. These cells contain saturable, high affinity (KA = 7 x 10(8) LM(-1)) binding sites for Tx and specific for antiestrogens (12,000 sites/cell). The concentration of these sites is significantly decreased in Tx-resistant variants of this cell line (2,700 sites/cell). In contrast, the concentration of estrogen receptors is similar in this population of variants and in the wild population (43,000 and 48,000 sites/cell respectively). These data support the concept of a role of these antiestrogen binding sites in the molecular mechanism controlling the multiplication of these breast cancer cells.
D001943:Breast Neoplasms / Q000503:physiopathology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D005260:Female; D006801:Humans; D007700:Kinetics; D011955:Receptors, Drug / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D013629:Tamoxifen / Q000378:metabolism / Q000494:pharmacology*
D003470:Culture Media; D011955:Receptors, Drug; D011960:Receptors, Estrogen; D013629:Tamoxifen
Etude du mécanisme d'action d'un antioestrogène du groupe triphényléthylène sur la croissance de la lignée cellulaire de cancer du sein humain MCF-7 en culture.