Identification of a 16-S RNA fragment crosslinked to protein S1 within Escherichia coli ribosomal 30-S subunits by the use of a crosslinking reagent: ethyl 4-azidobenzoylaminoacetimidate.
The bifunctional reagent ethyl 4-azidobenzoylaminoacetimidate was used to crosslink specifically ribosomal protein S1 to 16-S RNA within 30-S subunits. The reagent was attached to isolated protein S1. The modified protein was reassociated with protein-S1-depleted 30-S subunits and then crosslinked to the RNA molecule. The covalently bound 16-S RNA-protein S1 complex was isolated and the RNA fragment C-U-A-A-C-G-C-G-U-U-A-A-G-U-C-G-A-C-C-G-C-C-U-G-G-G-G-A-G (positions 861-889) was characterized to be crosslinked to protein S1.
D001483:Base Sequence; D003432:Cross-Linking Reagents; D004926:Escherichia coli / Q000032:analysis*; D007096:Imidoesters; D046911:Macromolecular Substances; D009843:Oligoribonucleotides / Q000032:analysis; D011485:Protein Binding; D012335:RNA, Ribosomal / Q000032:analysis*; D006163:Ribonuclease T1; D012269:Ribosomal Proteins / Q000032:analysis*; D012270:Ribosomes / Q000032:analysis*
D003432:Cross-Linking Reagents; D007096:Imidoesters; D046911:Macromolecular Substances; D009843:Oligoribonucleotides; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; C015494:ribosomal protein S1; C027921:ethyl 4-azidobenzoylaminoacetimidate; D006163:Ribonuclease T1