[New possibility in enzymatic technology: hydroxylase activity of immobilized hemoglobin].
Hydroxylation of Aniline by haemoglobin immobilized as cross-linked soluble polymers and insoluble particles was studied. Activity yields after immobilization as well as kinetic constants were estimated. Hydroxylase activities similar to liver microsomal cytochrome P450 activities were obtained.
D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D002417:Cattle; D004800:Enzymes, Immobilized / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D006900:Hydroxylation; D007700:Kinetics; D008706:Methemoglobin / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010108:Oxyhemoglobins / Q000378:metabolism
D000814:Aniline Compounds; D004800:Enzymes, Immobilized; D006454:Hemoglobins; D010108:Oxyhemoglobins; D008706:Methemoglobin; D006899:Mixed Function Oxygenases
Une nouvelle possibilité en technologie enzymatique: l'activité hydroxylase de l'hémoglobine immobilisée.