Two specific ribonucleoprotein fragments from rat liver 60S ribosomal subunits.
K+-depleted 60S ribosomal subunits from rat liver were submitted to a mild treatment with ribonuclease T1. Ribonucleoprotein fragments could be separated on sucrose gradients only when the digested subunits were partially deproteinized with a high KCl concentration (0.6 M) which removed seven proteins more or less completely and 5S RNA. The RNA and protein content of each fragment has been characterized. The largest ribonucleoprotein enclosed two RNA fragments of about 950,000 and 750,000 daltons and all the salt-resistant proteins except L5. The smallest one enclosed protein L5 (with L11, L17 and L26 in small amounts) and a 67,000 RNA piece. The subsequent hydrolysis of the large ribonucleoprotein produced several other ribonucleoproteins. One of them has been fully characterized: it enclosed a 250,000 RNA fragment and protein L12 (with L11, L25 and L30 in smaller amounts).
D000818:Animals; D002499:Centrifugation, Density Gradient; D008099:Liver / Q000032:analysis*; D008297:Male; D008970:Molecular Weight; D009698:Nucleoproteins / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D006163:Ribonuclease T1 / Q000378:metabolism; D012261:Ribonucleoproteins / Q000302:isolation & purification*; D012270:Ribosomes / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D009698:Nucleoproteins; D012261:Ribonucleoproteins; D006163:Ribonuclease T1