title: Ferrisiderophore reductase activity associated with an aromatic biosynthetic enzyme complex in Bacillus subtilis.
abstract: The cytoplasmic fractions obtained from Bacillus subtilis strains W168 and WB2802 catalyzed reductive release of iron from the ferric chelate of 2,3-dihydroxybenzoic acid (ferri-DHB), the ferrisiderophore produced by B. subtilis. Ferrisiderophore reductase activity may insert iron into metabolism. This activity required a reductant (reduced nicotinamide adenine dinucleotide phosphate was preferred), was oxygen sensitive, and was stimulated by flavin mononucleotide plus certain divalent cations. The cytoplasmic fractions also reduced 2,6-dichlorophenolindophenol; this reaction was stimulated by flavin mononucleotide plus a divalent cation. Ferri-DHB and 2,6-dichlorophenolindophenol reductase activities were copurified by phosphocellulose and diethylaminoethyl-cellulose chromatography. Nondenaturing polyacrylamide gel electrophoresis of the purified material revealed that both ferri-DHB and 2,6-dichlorophenolindophenol reductase activities were located in a protein band at Rf 0.75. The chromatographic procedures purified a reductase known to be associated with two aromatic biosynthetic enzymes, chorismate synthase and dehydroquinate synthase. Therefore, a portion of the ferrisiderophore reductase activity in B. subtilis may be catalyzed by a reductase that also is essential for aromatic biosynthesis.
mesh_terms: D015086:2,6-Dichloroindophenol / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology*; D002413:Cations, Divalent / Q000494:pharmacology; D002827:Chorismic Acid / Q000378:metabolism; D005486:Flavin Mononucleotide / Q000494:pharmacology; D062385:Hydroxybenzoates / Q000378:metabolism; D007502:Iron Chelating Agents / Q000378:metabolism; D008190:Lyases / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases; D010088:Oxidoreductases / Q000378:metabolism*; D019761:Phosphorus-Oxygen Lyases; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D002413:Cations, Divalent; D062385:Hydroxybenzoates; D007502:Iron Chelating Agents; D011801:Quinic Acid; C009135:2,3-dihydroxybenzoic acid; D005486:Flavin Mononucleotide; D015086:2,6-Dichloroindophenol; D010088:Oxidoreductases; D009247:NADH, NADPH Oxidoreductases; C024253:ferrisiderophore reductase; D008190:Lyases; C019895:chorismate synthase; D019761:Phosphorus-Oxygen Lyases; C023150:5-dehydroquinate synthetase; D002827:Chorismic Acid