Purification of polypeptide P6 with a molecular weight of 6,000 from the vegetative chromosomes of Bacillus subtilis.
Folded chromosomes were prepared as membrane-associated complexes from vegetative cells of Bacillus subtilis by stepwise sucrose gradient centrifugation. From nucleoids, a deoxyribonucleic acid-bound polypeptide with a molecular weight of 6,000 (P6) was purified by KCl-(NH4)2SO4 salting out, diethylaminoethyl cellulose column chromatography, and deoxyribonucleic acid cellulose column chromatography. The amino acid composition of polypeptide P6 was determined.
D000596:Amino Acids / Q000032:analysis; D001412:Bacillus subtilis / Q000032:analysis*; D001426:Bacterial Proteins / Q000302:isolation & purification; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis*; D002876:Chromosomes, Bacterial / Q000032:analysis*; D004269:DNA, Bacterial / Q000378:metabolism; D008970:Molecular Weight; D011485:Protein Binding
D000596:Amino Acids; D001426:Bacterial Proteins; D002868:Chromosomal Proteins, Non-Histone; D004269:DNA, Bacterial