title: Isolation of two cholic acid-binding proteins from rat liver cytosol using affinity chromatography.
abstract: Using an affinity matrix coupled with cholic acid, two proteins that recognise bile acids were isolated from rat liver cytosol. One protein of molecular weight 68 000 was immunologically identical to rat albumin. The other protein was of molecular weight 46 000. On discontinuous sodium dodecyl sulphate-polyacrylamide gel electrophoresis the 46 000 molecular weight protein dissociated to a single band with an RF value identical to the Yb subunit of the bromosulphophthalein-binding fraction (Y-fraction) of whole liver cytosol. The monomers of purified ligandin under these conditions resolved into two bands which corresponded to the Ya and Yc subunits of liver cytosol Y-fraction. Anti-serum to the purified ligandin reacted monospecifically with purified ligandin and whole liver cytosol, but did not cross-react with the Yb dimer eluted from the affinity column. The Yb dimer was shown to possess glutathione-S-transferase activity with a substrate specificity distinct from ligandin but similar to glutathione-S-transferase C. Cholic acid inhibited the catalytic activity of the transferase.
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification*; D002793:Cholic Acids / Q000302:isolation & purification* / Q000494:pharmacology; D002846:Chromatography, Affinity; D003600:Cytosol / Q000032:analysis; D005982:Glutathione Transferase / Q000302:isolation & purification*; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008099:Liver / Q000032:analysis*; D046911:Macromolecular Substances; D008297:Male; D008970:Molecular Weight; D051381:Rats; D013379:Substrate Specificity
chemical_list: D002352:Carrier Proteins; D002793:Cholic Acids; D046911:Macromolecular Substances; C029734:cholic acid-binding proteins; D005982:Glutathione Transferase