[Properties of beta-glucosidase from the cellulolytic fungus Geotrichum candidum 3c].
beta-Glucosidase (beta-D-glucoside glucohydrolase, EC 3.2.1.21) splitting p-nitrophenyl-beta-D-glucosidase was isolated from cellular preparation of the fungus Geotrichum candidum 3c "cellocandine G10x" and purified 38-fold. The enzyme was homogeneous during ultracentrifugation, gel-filtration, isoelectrofocusing and disc-electrophoresis in polyacrylamide gel and had a pI of 4.2, sedimentation coefficient of 2.6S and molecular weight of 120 000. The enzyme had maximal activity at pH 5.6, 45 degrees and retained up to 45% of its activity under optimal conditions (pH, t degrees) after 48 hr incubation. beta-Glucosidase did not split disaccharides, e. g. lactose cellobiose, laminaribiose, gentibiose as well as o-nitrophenyl-beta-D-galactoside, methyl-beta-D-xyloside. The enzyme hydrolyzed p-nitrophenyl-beta-D-glucopyranoside (Km = 0.18 mM) and some plant beta-glucosides, such as phenylethyl-beta-D-glucopyranoside from rose petals and diosgenine tetrasaccharide from deltoid dioscorea (Km = 0.26 mM).
D002480:Cellulase / Q000302:isolation & purification / Q000378:metabolism; D005848:Geotrichum / Q000201:enzymology*; D005959:Glucosidases / Q000378:metabolism*; D007700:Kinetics; D003904:Mitosporic Fungi / Q000201:enzymology*; D008970:Molecular Weight; D013379:Substrate Specificity; D001617:beta-Glucosidase / Q000302:isolation & purification / Q000378:metabolism*
D005959:Glucosidases; D001617:beta-Glucosidase; D002480:Cellulase
Svoĭstva beta-gliukozidazy iz tselliuloliticheskogo griba Geotrichum candidum 3c.