[Use of the ex vivo vascular perfusion technique in the rat for the study of the intestinal absorption of fatty acids into the blood].
Lipid emulsion containing tritiated decanoic or oleic acid was infused in an isolated intestinal loop in situ. An ex vivo vascular perfusion technique has been used, allowing the continuous sampling of mesenteric portal blood. With decanoic acid the amount of radioactivity recovered in portal blood was high and similar for actidione-cycloheximide treated and control Rats. On the contrary the amount of radioactivity recovered in control Rats after oleic acid absorption was low. These results show that the ex vivo vascular perfusion technique can be used to study the non-esterified fatty acid intestinal absorption.
D000818:Animals; D005227:Fatty Acids / Q000097:blood / Q000378:metabolism*; D007408:Intestinal Absorption; D008297:Male; D010477:Perfusion / Q000379:methods; D051381:Rats
D005227:Fatty Acids
Utilisation de la technique de perfusion vasculaire ex vivo chez le rat pour l'étude de l'absorption intestinale des acides gras par la voie sanguine.