[Different accessibility to RNase T1 of 5S- and 5.8S-rRNA in 60S subunits and 80S ribosomes of yeast].
The accessibility of RNase T1 hydrolysis of small rRNAs in 60S subunits and 80S yeast cytoplasmic ribosomes was studied. It was shown that in 60S subunit 5S and 5.8S rRNAs are hydrolyzed by RNase T1, whereas in 80S ribosome no hydrolysis occurs. The participation of small rRNAs in the association of ribosomal subunits is discussed.
D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D007700:Kinetics; D008970:Molecular Weight; D012335:RNA, Ribosomal / Q000032:analysis*; D006163:Ribonuclease T1 / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism*; D012270:Ribosomes / Q000648:ultrastructure*; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure*
D012335:RNA, Ribosomal; D012260:Ribonucleases; D006163:Ribonuclease T1
Razlichnaia dostupnost' 5S- i 6.8S-rRNK dlia RNKazy T1 v 60S-subchastitsakh i 80S-ribosomakh drozhzheĭ.