Immunoglobulin heavy-chain expression and class switching in a murine leukaemia cell line.
A cell line that switches from mu to gamma 2b synthesis during growth in culture uses the same VH region for both heavy chains but retains two copies of the Cmu gene. This suggests that the mu to gamma 2b class switch can occur, at least in part, by an RNA processing mechanism. Regulatory variants of this cell line lose constitutive mu-chain synthesis but simultaneously acquire lipopolysaccharide (LPS)-inducible synthesis of that chain. This co-variation is allele-specific and is correlated to a large deletion of DNA in the JH--Cmu intron.
D000818:Animals; D000916:Antibody Diversity; D002460:Cell Line; D002872:Chromosome Deletion; D005786:Gene Expression Regulation; D005796:Genes; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007142:Immunoglobulin gamma-Chains / Q000235:genetics*; D007148:Immunoglobulin mu-Chains / Q000235:genetics*; D007942:Leukemia, Experimental / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D012326:RNA Splicing; D011995:Recombination, Genetic
D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D007142:Immunoglobulin gamma-Chains; D007148:Immunoglobulin mu-Chains; D008070:Lipopolysaccharides