Morphological transformation of C3H/10T1/2 CL8 cells by procarcinogens.
In order to increase the sensitivity of the C3H/10T1/2 CL8 (10T1/2) cell transformation system, we increased the chemical exposure period to a total of 6 days (two consecutive 3-day exposures). Using this modified procedure, we transformed 10T1/2 cells with procarcinogens such as aflatoxin B1, benz(a)anthracene, and 4-nitroquinoline-1-oxide which have been negative in the standard 10T1/2 cell transformation assay. However, beta-naphthylamine was inconclusive and 2-acetylamino-fluorine was negative in this modified assay system. Our results demonstrate that a simple modification of the 10T1/2 cell transformation method can increase the sensitivity to some procarcinogens that require metabolic activation.
D015073:2-Acetylaminofluorene / Q000494:pharmacology; D015081:2-Naphthylamine / Q000494:pharmacology; D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D016604:Aflatoxin B1; D000348:Aflatoxins / Q000494:pharmacology; D000818:Animals; D001551:Benz(a)Anthracenes / Q000494:pharmacology; D001711:Biotransformation; D002273:Carcinogens / Q000494:pharmacology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004622:Embryo, Mammalian; D051379:Mice; D013997:Time Factors
D000348:Aflatoxins; D001551:Benz(a)Anthracenes; D002273:Carcinogens; D015112:4-Nitroquinoline-1-oxide; D015073:2-Acetylaminofluorene; D016604:Aflatoxin B1; C030935:benz(a)anthracene; D015081:2-Naphthylamine