Oral mucosal carcinogenesis in hamsters: inhibition by vitamin E.
Eighty young adult male and female Syrian golden hamsters (Mesocricetus auratus) were divided into 4 equal experimental groups. In group I animals the left buccal pouch was painted three times weekly with a 0.5% solution of 7,12-dimethylbenz[a]anthracene (DMBA) in heavy mineral oil. In group 2 animals the left buccal pouch was similarly painted with DMBA, but the animals also received orally 10 mg vitamin E (dl-alpha-tocopherol) in peanut oil twice weekly on alternate days with DMBA painting. Group 1 animals received a similar amount of peanut oil vehicle, group 3 animals received only vitamin E (vitamin E controls) in peanut oil, and group 4 animals served as untreated controls receiving only peanut oil. Four animals in each group (2 males and 2 females) were killed at 8, 10, 12, 14, and 16 weeks. Buccal pouches were photographed and excised, and tumors were noted and measured in the left buccal pouches. In group 2 animals receiving vitamin E, tumor formation was significantly delayed, so that by 14-16 weeks there were fewer tumors and their average size was smaller than that of tumors in group 1 animals that were painted with DMBA but received no vitamin E supplement. In group 2 there was also less invasion of underlying tissues and less surface necrosis.
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D002610:Cheek; D006224:Cricetinae; D004032:Diet; D004347:Drug Interactions; D005260:Female; D008297:Male; D008647:Mesocricetus; D009061:Mouth Mucosa; D009062:Mouth Neoplasms / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D013997:Time Factors; D014810:Vitamin E / Q000008:administration & dosage*
D014810:Vitamin E; D015127:9,10-Dimethyl-1,2-benzanthracene