The biological activity of different gastrin peptides assessed using a cytochemical section bioassay.
A cytochemical section bioassay has been used to assess the carbonic anhydrase activity, induced by the gastrin peptides G13, G17 and G34 (concentration 5.0-0.005 pg/ml), in guinea pig parietal cells. When the mean integrated density of the induced response x 100, was plotted against the concentration of the gastrin peptide used, a graded dose response and a positive linear correlation was found for G13 and G17. These responses were similar. G34 did not produce a linear dose response in the concentration range 5.0-0.005 pg/ml, but a higher overall carbonic anhydrase activity was observed than with G13 and G17.
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002256:Carbonic Anhydrases / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005748:Gastric Fundus / Q000201:enzymology; D005755:Gastrins / Q000032:analysis*; D006168:Guinea Pigs; D006651:Histocytochemistry; D008297:Male
D005755:Gastrins; D002256:Carbonic Anhydrases