[Biosynthesis of extracellular phospholipase C (lecithinase) from Bacillus cereus depending on the nutrient medium composition and pH].
The nutrient medium to provide rapid growth of microbial cells of Bacillus cereus str. 504, and biosynthesis of extracellular phospholipase C(EC 3.1.4.3) was selected. The nutrient medium contained acidic casein hydrolyzate, yeast extract or enzymatic hydrolyzate of fodder yeast, glucose, NaCl, NaHCO3, and Na2HPO4. The activity of secreted phospholipase C reached maximum at pH 6.3--6.6.
D001409:Bacillus cereus / Q000201:enzymology* / Q000254:growth & development; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010740:Phospholipases / Q000096:biosynthesis*; D010738:Type C Phospholipases / Q000096:biosynthesis*
D003470:Culture Media; D010740:Phospholipases; D010738:Type C Phospholipases
Binosintez vnekletochnoĭ fosfolipazy S (letsitinazy) Bacillus cereus v zavisimosti ot sostava pitatel'noĭ spedy i pH.