Measurement of alum precipitated allergen extracts of RAST inhibition methods.
The feasibility of measuring the allergenic activities of alum precipitated pollen extracts by means of the radioallergosorbent test (RAST) was investigated. Different batches of aqueous and alum precipitated birch and mixed grass pollen extracts were used as antigens. The alum precipitates could be readily dissolved with 0.1 M EDTA (pH 9.0) without any loss in allergenicity. No significant differences in RAST activities of undissolved and dissolved alum precipitated allergen extracts could be detected in terms of ability to inhibit the binding of allergen specific IgE to solid-phase allergens.
D000485:Allergens; D000536:Aluminum Hydroxide / Q000494:pharmacology*; D002241:Carbohydrates / Q000276:immunology; D011232:Chemical Precipitation; D004492:Edetic Acid / Q000494:pharmacology; D010940:Plant Proteins / Q000276:immunology; D006109:Poaceae / Q000276:immunology; D011725:Pyridines / Q000494:pharmacology; D011852:Radioallergosorbent Test
D000485:Allergens; D002241:Carbohydrates; D010940:Plant Proteins; D011725:Pyridines; D000536:Aluminum Hydroxide; D004492:Edetic Acid