[Lipase and phospholipase C from Staphylococcus aureus of different origin. I. Determination and occurrence (author's transl)].
Lipase and phospholipase C from Staphylococcus aureus of different origin were demonstrated qualitatively by agar diffusion on tributyrin- and lecithin agar. On test media with either 0,3% Na-azide or 0,3% KCN lipase-activity was not inhibited, phospholipase C, on the other hand, completely blocked (Table 1, Fig. 2). In this manner a tentative differentiation was possible between lipase and phospholipase C. For the quantitative determination of lipase the hydrolysis of p-nitrophenyl palmitate proved to be most useful (Fig. 1). S. aureus-cultures of human origin produced more often and more actively lipase and phospholipase C than those from cattle (Table 2).
D000818:Animals; D002417:Cattle; D005260:Female; D005779:Immunodiffusion; D008049:Lipase / Q000032:analysis* / Q000378:metabolism; D008414:Mastitis, Bovine / Q000382:microbiology; D010740:Phospholipases / Q000032:analysis*; D013045:Species Specificity; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000201:enzymology*; D010738:Type C Phospholipases / Q000032:analysis* / Q000378:metabolism
D010740:Phospholipases; D008049:Lipase; D010738:Type C Phospholipases
Lipase und Phospholipase C von Staphylococcus aureus verschiedener Herkunft.I. Nachweis und Vorkommen.