A method for the purification of bovine vitreous body hyaluronic acid.
Bovine vitreous body hyaluronic acid was treated with activated CH-Sepharose 4B after purification with cold 5% trichloroacetic acid. By this method a highly purified hyaluronic acid can be prepared. Contaminating serum proteins were completely removed. The remaining small amounts (0.3%) of the protein contaminants were found, by immunological methods, to be non-plasma proteins.
D000818:Animals; D002417:Cattle; D006820:Hyaluronic Acid / Q000302:isolation & purification*; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D014783:Viscosity; D014822:Vitreous Body / Q000032:analysis*
D006820:Hyaluronic Acid