Isolation of Fasciola hepatica tegument antigens.
Fasciola hepatica tegument antigens were isolated from intact worms in the cold by using Nonidet P-40. Proof of the tegumental nature of the antigens was shown by the peroxidase-antiperoxidase immunocytochemical technique at the light microscope level. The potential of F. hepatica tegument antigens for the immunodiagnosis of rabbit and human fascioliasis was shown by Ouchterlony immunodiffusion, although cross-reactivity was evident in one of six serum samples from patients infected with Schistosoma mansoni. A genus-specific Fasciola antigen was found in F. hepatica tegument. Finally, F. hepatica tegument contained antigens which protected mice from challenge infection with S. mansoni.
D000818:Animals; D000941:Antigens / Q000276:immunology / Q000302:isolation & purification*; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000175:diagnosis; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D011817:Rabbits; D012552:Schistosomiasis / Q000276:immunology; D012698:Serologic Tests
D000941:Antigens