[Single step isolation of a hemoglobin binding protein present in chicken plasma].
A one step procedure for the purification of a Chicken hemoglobin binding protein is reported here for the first time. The method includes a single affinity chromatography step on Sepharose coupled to Chicken hemoglobin. The protein obtained is pure, its ability of binding hemoglobin is maintained and the recovery is close to 100%. Moreover, the same affinity system may be used again.
D000818:Animals; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000302:isolation & purification*; D002645:Chickens; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D007123:Immunoelectrophoresis, Two-Dimensional
D001426:Bacterial Proteins; D002352:Carrier Proteins; C017206:hemoglobin-binding protein, bacteria
Isolement en une seule étape d'une protéine liant l'hémoglobine présente dans la plasma de poule.