[Comparative study of the immunochemical properties of the 5th lactate dehydrogenase isoenzyme from rat liver and skeletal muscle. II. Isolation and characteristics of LDH-5 preparations from rat liver. General analysis of the results].
The method of isolation of chromatographically homogenic LDG-5 preparation from the rat liver was elaborated. The preparations obtained in the present study and LDG-5 preparations from skeletal muscles have a common component which is testified by results of the precipitation test in agar. In the rat liver there is an enzymatically non-active antigenic component, which, according to some of its physico-chemical parameters, is very similar with chemically modified forms of LDG-5 dimers.
D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005779:Immunodiffusion; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000276:immunology / Q000302:isolation & purification*; D008099:Liver / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology; D009928:Organ Specificity; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D013057:Spectrum Analysis
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase
Sravnitel'noe izuchenie immunokhimicheskikh svoĭstv piatogo izozima laktatdegidrogenazy iz skeletnykh myshts i pecheni krys. II. Poluchenie i kharakteristika preparatov LDG-5 iz pecheni krysys. Obshchiĭ analiz rezul'tatov.