Simple, inexpensive, and rapid way to produce Bacillus subtilis spores for the Guthrie bioassay.
Esculin agar has been found to be a simple, inexpensive, rapid, and reliable means to promote production of spores of inhibitor-sensitive clones of Bacillus subtilis strains ATCC 6051 and 6633 for use in the Guthrie bioassay screening tests for genetic metabolic disorders.
D000362:Agar; D001412:Bacillus subtilis / Q000254:growth & development*; D001681:Biological Assay / Q000379:methods*; D004929:Esculin; D006358:Hot Temperature; D006801:Humans; D008661:Metabolism, Inborn Errors / Q000175:diagnosis*; D013171:Spores, Bacterial
D004929:Esculin; D000362:Agar