title: Barriers to water flow in vasopressin-treated toad urinary bladder.
abstract: Unstirred layers of water complicate the measurement of water permeability across epithelia. In the toad urinary bladder, the hormone vasopressin increases the osmotic water permeability of the granular epithelial cell's luminal membrane, and also leads to the appearance of aggregates of particles within this membrane. The aggregates appear to be markers for luminal membrane osmotic water permeability. This report analyzes the relationship between transbladder osmotic water flow and aggregate frequency, and demonstrates that flow across the bladder is significantly attenuated by unstirred layers of water or by structural barriers other than the luminal membrane when the luminal membrane is made permeable by vasopressin. This analysis in addition yields unique values for the permeabilities of both the luminal membrane and the barriers to water flow which lie in series with it.
mesh_terms: D000818:Animals; D002024:Bufo marinus; D002463:Cell Membrane Permeability; D004848:Epithelium / Q000378:metabolism; D005260:Female; D009995:Osmosis; D013499:Surface Properties; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism*; D014667:Vasopressins / Q000494:pharmacology*; D014867:Water / Q000378:metabolism*
chemical_list: D014867:Water; D014667:Vasopressins