title: Pulmonary carbonic anhydrase in the human, monkey, and rat.
abstract: The distribution of carbonic anhydrase in the human, monkey, and rat lung was studied by the histochemical method of Hansson. High activity of this enzyme was demonstrated in the endothelium of pulmonary capillaries. In the human and the monkey lung enzyme activity was exhibited in the whole circumference of the capillaries, but in the rat enzyme activity is confined to capillary segments having close contact with alveolar epithelium forming the blood-air barrier. Staining was inhibited by 10 microM acetazolamide, but was not affected by 10 microM Cl 13,850, an inactive acetazolamide analogue. The location of carbonic anhydrase in the lung supports the idea that pulmonary carbonic anhydrase promotes CO2 elimination from the blood into the alveolar space. Its possible functions may be to act upon plasma to accelerate the conversion of HCO-3 to CO2 and to facilitate CO2 transport through the lung tissue.
mesh_terms: D000368:Aged; D000818:Animals; D001980:Bronchi / Q000166:cytology / Q000201:enzymology; D002196:Capillaries / Q000201:enzymology; D002256:Carbonic Anhydrases / Q000378:metabolism*; D004727:Endothelium / Q000201:enzymology; D004848:Epithelium / Q000201:enzymology; D006651:Histocytochemistry; D006801:Humans; D008168:Lung / Q000201:enzymology*; D008252:Macaca fascicularis; D008875:Middle Aged; D011650:Pulmonary Alveoli / Q000201:enzymology; D011652:Pulmonary Circulation; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002256:Carbonic Anhydrases