A rapid solid-phase enzyme-linked binding assay for screening monoclonal antibodies to cell surface antigens.
A solid-phase enzyme-linked binding assay is described for screening monoclonal antibodies to cell surface antigens. E. coli beta-galactosidase was coupled to rabbit anti-rat Ig and used to detect the binding of rat monoclonal antibodies to cells which had been fixed to the wells of microtitre plates using a combination of poly-L-lysine and glutaraldehyde. This method was found to be advantageous for the large screening of monoclonal antibodies with a panel of cell types, and has been useful in the selection of antibodies which would be candidates for differentiation markers within the human and mouse haemopoietic systems.
D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface; D002448:Cell Adhesion; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005976:Glutaral / Q000494:pharmacology; D007136:Immunoglobulins; D007185:Incubators; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D001616:beta-Galactosidase / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007136:Immunoglobulins; D001616:beta-Galactosidase; D005976:Glutaral