Glycoproteins containing peanut-agglutinin receptors from human-peripheral-blood T-lymphocyte plasma membranes.
A glycoprotein fraction possessing peanut agglutinin receptors has been isolated from detergent extracts of neuraminidase-treated human peripheral blood T-lymphocyte plasma membranes with affinity matrices comprising the peanut agglutinin co-valently immobilised on Sepharose 4B. This fraction could be specifically eluted from affinity columns using buffer solutions supplemented with 0.2 M D-galactose and was shown, by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (0.1%), to contain four major glycoprotein components with apparent molecular weights of 200000, 190000, 110000 and 95000. It is suggested, from the observed reactivity of these glycoproteins with various lectins in double-diffusion experiments, that they possess both O-glycosidically and N-glycosidically linked carbohydrates.
D002462:Cell Membrane / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D005779:Immunodiffusion; D037102:Lectins; D008970:Molecular Weight; D017830:Octoxynol; D019887:Peanut Agglutinin; D011092:Polyethylene Glycols; D011975:Receptors, Mitogen / Q000032:analysis*; D013601:T-Lymphocytes / Q000032:analysis*
D006023:Glycoproteins; D037102:Lectins; D019887:Peanut Agglutinin; D011975:Receptors, Mitogen; D011092:Polyethylene Glycols; D017830:Octoxynol