Purification and properties of anti-Trypanosoma cruzi antibodies isolated from patients with chronic Chagas' disease.
Three purified human IgG antibodies against Trypanosoma cruzi (anti-F, anti-Mc and anti-Cs) showed different reactivities in vitro. While all of them agglutinated specifically sensitized sheep red cells the complement-fixing capacity of anti-Fc and anti-Cs was higher than that of anti-Mc antibody. This one also showed a minimal precipitating activity. When the ability to neutralize mice bloodstream trypomastigotes was analyzed, the results obtained indicated that anti-F antibody was the most effective.
D000906:Antibodies / Q000302:isolation & purification*; D014355:Chagas Disease / Q000276:immunology*; D002908:Chronic Disease; D003168:Complement Fixation Tests; D003593:Cytoplasm / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005407:Flagella / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G; D008861:Microsomes / Q000276:immunology
D000906:Antibodies; D007074:Immunoglobulin G