Cellular effects of human leukocyte hydrolases. II. Ultrastructural studies on fibroblasts.
Morphologic ultrastructural changes induced in human gingival fibroblasts in vitro by treatment with human neutrophil granule enzymes were studied. Surface mucopolysaccharide of the fibroblasts was examined by ruthenium red staining. The only alteration that could be identified was the removal of surface mucopolysaccharide after treatment with granule enzymes, but not when soybean trypsin inhibitor was present. When the cells were released from their growth surface they assumed the characteristics of floating cells rather than spreading fibroblasts, but were otherwise unremarkable.
D000328:Adult; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000648:ultrastructure*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D006867:Hydrolases / Q000494:pharmacology*; D008247:Lysosomes / Q000201:enzymology; D008854:Microscopy, Electron; D009504:Neutrophils / Q000201:enzymology*; D013997:Time Factors
D006867:Hydrolases