title: Effect of LH and FSH on testosterone release from cultured Leydig cells.
abstract: The level of testosterone in incubation media of cultures of Leydig cells isolated from mouse testes were measured by specific radioimmunoassay. Luteinizing hormone (LH) in a concentration of 10, 100 and 500 ng per 1 ml of culture medium stimulated the secretion of testosterone very markedly especially after 2 days of culture. LH in the amount of 100 ng ml-1 exerted the most stimulating effect on testosterone production by cultured Leydig cells. Follicle stimulating hormone (FSH) in a concentration 100 ng ml-1 of culture medium did not influence significantly the androgen production.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D007985:Leydig Cells / Q000187:drug effects / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000494:pharmacology*; D008297:Male; D051379:Mice; D013739:Testosterone / Q000378:metabolism*
chemical_list: D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone