Enzyme-linked immunosorbent assay compared with immunoprecipitation for serotyping Neisseria meningitidis and its use in demonstrating serotype polymorphism.
Rabbit antisera generated against 11 prototype strains of group B Neisseria meningitidis were tritiated by using the enzyme-linked immunosorbent assay and the Ouchterlony immunoprecipitation technique. The relative insensitivity of the immunoprecipitation technique in detecting antibody compared with the enzyme-linked immunosorbent assay was demonstrated. Cross-reactions within the group and among the 11 serotypes were characterized with respect to established serological techniques. Serotype polymorphism among the strains of N. meningitidis was demonstrated with the enzyme-linked immunosorbent assay.
D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005779:Immunodiffusion; D009345:Neisseria meningitidis / Q000145:classification* / Q000276:immunology; D011110:Polymorphism, Genetic; D012703:Serotyping / Q000379:methods*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial