Evaluation of the coagglutination test for the identification of Neisseria gonorrhoeae in primary cultures.
The coagglutination (COA) test for the identification of Neisseria gonorrhoeae was compared with immunofluorescence and sugar degradation tests on 1710 gonococcal isolates, 72 of which produce beta-lactamase. The COA test gave a positive result for 98.6% of the strains. Treatment of suspensions with Streptomyces enzyme reduced the incidence of inconclusive results due to autoagglutination to 1.2%. Cross-reactivity of the gonococcal antiserum was minimised by absorption with meningococci and Moraxella species. The COA provide a simple, quick, and reliable method for identifying N gonorrhoeae in culture.
D000372:Agglutination Tests / Q000379:methods; D050260:Carbohydrate Metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification* / Q000378:metabolism