title: A heterophile antigen associated with experimental toxoplasmosis.
abstract: The biological characteristics of a heterophile protein (HP) in peritoneal exudate from mice, hamsters, rats, and guinea pigs infected with Toxoplasma gondii were studied by immunofluorescence, immunoelectrophoresis and immunodiffusion techniques using specific antisera raised in rabbits. HP of mice had the highest antigenicity, HP of hamsters and rats had intermediate antigenicity and HP of guinea pigs had the lowest antigenicity. HP was found in normal peritoneal exudates from mice, hamsters and rats inoculated with paraffin oil instead of T. gondii and in normal guinea pig serum. HP was detected by the fluorescent antibody technique on the surface of T. gondii in peritoneal exudates of mice, but not on mouse peritoneal cells, and by the indirect fluorescent antibody technique on L cells infected with T. gondii and on free Toxoplasma derived from them, but not on uninfected L cells. T. gondii could make host cells produce HP to cover its surface for protection. The relation between HP from host cells and T. gondii is discussed.
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis*; D001202:Ascitic Fluid / Q000276:immunology; D006224:Cricetinae; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D051379:Mice; D051381:Rats; D014122:Toxoplasma / Q000276:immunology; D014124:Toxoplasmosis, Animal / Q000276:immunology*
chemical_list: D000941:Antigens