The capacity of activated murine macrophages for augmented binding of neoplastic cells: analysis of induction by lymphokine containing MAF and kinetics of the reaction.
The capacity for augmented binding of tumor cells is an initial and necessary part of macrophage-mediated tumor cytotoxicity. To study the induction of binding capacity, we obtained FCS-elicited, inflammatory macrophages from C57BL/6J mice. Exposure of these macrophages to lymphokine(s) containing MAF induced augmented binding capacity in a dose-dependent fashion. Resident peritoneal macrophages did not respond to lymphokine, and endotoxin did not appreciably influence induction of binding. Maximum induction of binding required continuous interaction between macrophages and lymphokine for 6 to 10 hr. The conditions necessary for induction of binding closely paralleled those for induction of priming or cytolysis. Exposure of FCS-elicited macrophages from C3H/HeJ mice, although not of macrophages from A/J mice, induced augmented binding. The data suggest that the augmented capacity for binding tumor cells is induced by lymphokine(s) and that a major part of induction of priming for cytolysis by MAF is induction of such binding.
D000818:Animals; D001665:Binding Sites; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D004731:Endotoxins / Q000235:genetics / Q000494:pharmacology; D005260:Female; D007700:Kinetics; D008222:Lymphokines / Q000235:genetics / Q000494:pharmacology*; D008223:Lymphoma / Q000276:immunology*; D008262:Macrophage Activation; D016215:Macrophage-Activating Factors; D012515:Mast-Cell Sarcoma / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA
D004731:Endotoxins; D008222:Lymphokines; D016215:Macrophage-Activating Factors