title: Immunochemical studies of infectious mononucleosis--X. Characterization of a glycoprotein from horse erythrocytes which reacts with Paul-Bunnell antibody.
abstract: A highly purified preparation of horse erythrocyte glycoprotein was prepared from an aqueous ethanolic extract of hemoglobin-free membranes. The subunit apparent mol. wt was 30,000. In aqueous solution the glycoprotein formed globular aggregates of 93 +/- 16 A diameter. The glycoprotein had a receptor for the Paul-Bunnell antibody of infectious mononucleosis which was associated with an O-glycosidically linked oligosaccharide and dependent on the presence of N-glycolylneuraminic acid. In addition the glycoprotein had a neuraminidase-sensitive receptor for human peripheral blood lymphocytes. Fifty per cent inhibition of the rosetting of sheep red cells by 4 x 10(5) lymphocytes was caused by 30 microgram of glycoprotein.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology / Q000302:isolation & purification; D002241:Carbohydrates / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000276:immunology*; D006020:Glycopeptides / Q000302:isolation & purification; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification; D006736:Horses / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D007244:Infectious Mononucleosis / Q000276:immunology*; D008854:Microscopy, Electron; D012397:Rosette Formation
chemical_list: D000596:Amino Acids; D000914:Antibodies, Viral; D000954:Antigens, Surface; D002241:Carbohydrates; D006020:Glycopeptides; D006023:Glycoproteins