title: Antibodies to laminin in Chagas' disease.
abstract: We have found that sera from humans with Chagas' disease and Rhesus monkeys infected with Trypanosoma cruzi contain IgM and IgG antibodies, which react with structures in a variety of connective tissues. These antibodies react with laminin but not with various other purified connective tissue components like collagen types I, III, IV, and V, fibronectin, heparan sulfate (BM-1) proteoglycan, or chondronectin. The tissue-reacting antibodies were isolated by absorption to a laminin-Sepharose column. The bound fraction contained all the tissue-reacting antibodies. These antibodies strongly stained trypomastigotes and amastigotes, but weakly stained epimastigotes. These studies show that sera from T. cruzi-infected primates contain antilaminin antibodies, which may be produced by those host in response to a laminin-like molecule present in the parasite.
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000906:Antibodies; D002352:Carrier Proteins / Q000276:immunology; D014355:Chagas Disease / Q000276:immunology* / Q000473:pathology; D002675:Child, Preschool; D004587:Electrophoresis, Agar Gel; D004727:Endothelium / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007223:Infant; D007797:Laminin; D008253:Macaca mulatta; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D000906:Antibodies; D002352:Carrier Proteins; D006023:Glycoproteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007797:Laminin