title: Safety aspects in preparing suspensions of field strains of Brucella abortus for serological identification.
abstract: Forty field strains of Brucella abortus, obtained from milk and vaginal samples of naturally infected cattle, were heated in a waterbath at 65 degrees C, 75 degrees C, 80 degrees C for 60 to 120 minutes. Ninety-five per cent survived 65 degrees C for 120 minutes, 55 per cent survived 75 degrees C for 120 minutes, 25 per cent survived 80 degrees C for 120 minutes and 12.5 per cent survived 85 degrees C for 60 minutes. No isolates survived 85 degrees C for 75 minutes.
mesh_terms: D000818:Animals; D002003:Brucella abortus / Q000276:immunology / Q000302:isolation & purification*; D002007:Brucellosis, Bovine / Q000382:microbiology; D002417:Cattle; D003470:Culture Media; D005260:Female; D008892:Milk / Q000382:microbiology; D013696:Temperature
chemical_list: D003470:Culture Media