Protection of neocortical tissue prisms from freeze-thaw injury by dimethyl sulphoxide.
Neocortical tissue prisms prepared from rat and human brain were frozen to -196 degrees C by a two-step freezing procedure and 10% dimethyl sulphoxide as cryoprotectant. Frozen and thawed rat neocortical prisms incorporated glucose into acetylcholine and carbon dioxide at 89% and 86% of control values, respectively, and noradrenaline uptake into frozen and thawed rat prisms was 94% of the control value. Frozen and thawed prisms from three human neocortical specimens showed a similar degree of protection from freeze-thaw injury.
D000109:Acetylcholine / Q000096:biosynthesis; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005615:Freezing; D005947:Glucose / Q000378:metabolism; D006801:Humans; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats
D002245:Carbon Dioxide; D005947:Glucose; D000109:Acetylcholine; D009638:Norepinephrine; D004121:Dimethyl Sulfoxide