Effect of antigonadotropic sera on in vitro testicular metabolism of 3H-testosterone in immature rats.
Male rats were daily injected from the 5th day to the 21st day of life with purified rabbit anti-FSH serum, or anti-LH serum. After the last injection, they were killed and the testes incubated in vitro with 3H-testosterone. The radioactive metabolites were separated and identified by a double isotope dilution method. In rats treated with antigonadotropic sera, the total amount of 5 alpha-reduced metabolites was significantly decreased. The profile of the 5 alpha-reduced metabolites obtained in the anti-FSH treated group was different from those obtained in control and anti-LH treated groups. Antigonadotropic sera modify the in vitro metabolism of testosterone by the testicular tissue.
D015113:Androstane-3,17-diol / Q000378:metabolism; D000818:Animals; D013196:Dihydrotestosterone / Q000378:metabolism; D005640:Follicle Stimulating Hormone / Q000276:immunology*; D007106:Immune Sera; D007116:Immunization, Passive; D007986:Luteinizing Hormone / Q000276:immunology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism*
D007106:Immune Sera; D013196:Dihydrotestosterone; D015113:Androstane-3,17-diol; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone