Aminoguanidine reversal of the inhibitory effects of ornithine analogs on the in vitro clonogenic survival of the R3327AT prostate-derived tumor.
In in vitro studies with the Copenhagen rat prostate-derived tumor cell lines, the activity of ornithine decarboxylase (ODC) of these lines were as sensitive to inhibition by alpha-difluoromethylornithine (alpha-DFMO) as normal prostatic tissue. The in vitro growth of the tumor in calf serum was inhibited by ODC inhibitors alpha-methylornithine and alpha-difluoromethylornithine. This inhibition was partially reversed by the diamine oxidase inhibitor aminoguanidine. Further, alpha-DFMO was not inhibitory to the growth of these cells in sera which lacked diamine oxidase activity, but was inhibitory when diamine oxidase was added.
D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors* / Q000502:physiology; D000818:Animals; D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors*; D002460:Cell Line; D000518:Eflornithine; D006146:Guanidines / Q000494:pharmacology*; D008297:Male; D009952:Ornithine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009955:Ornithine Decarboxylase / Q000032:analysis; D065108:Ornithine Decarboxylase Inhibitors; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000201:enzymology; D011700:Putrescine / Q000494:pharmacology; D051381:Rats
D006146:Guanidines; D065108:Ornithine Decarboxylase Inhibitors; D009952:Ornithine; D006631:Amine Oxidase (Copper-Containing); D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; C004479:pimagedine; D011700:Putrescine; D000518:Eflornithine