Responder T cells depleted of alloreactive cells react to antigen presented on allogeneic macrophages from nonresponder strains.
T cells from strains responder to the antigen poly(Glu40 Ala60) (GA) were depleted of alloreactive cells by bromo-deoxyuridine and light treatment, and were subsequently primed in vitro in GA presented by allogeneic macrophages from nonresponder strains. Antigen-specific secondary proliferative responses restricted by allogeneic Ia molecules of the macrophages were obtained in all strain combinations tested. These data indicate that Ir gene-controlled nonresponsiveness cannot be the result of a failure of antigen presentation.
D000818:Animals; D005802:Genes, MHC Class II; D006168:Guinea Pigs; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008264:Macrophages / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D011948:Receptors, Antigen, T-Cell