The genes coding for tRNA Tyr of Drosophila melanogaster: localization of determination of the gene numbers.
Transfer RNA(Tyr) (anticodon G psi A) was isolated from Drosophila melanogaster by means of Sepharose 4B, RPC-5, and polyacrylamide gel electrophoresis. The rRNA was iodinated in vitro with Na125 I and hybridized in situ to salivary gland chromosomes from Drosophila. The genes of rRNA(Tyr) were localized in eight regions of the genome by autoradiography. Restriction enzyme analysis of genomic DNA indicated that the haploid Drosophila genome codes for about 23 tRNA(Tyr) genes. The regions 22F and 85A each contain four to five tRNA(Tyr) genes, whereas the regions 28C, 41AB, 42A, 42E, and 56D each contain two to three tRNA(Tyr) genes.
D000818:Animals; D001345:Autoradiography; D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure; D004330:Drosophila / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D005796:Genes; D009693:Nucleic Acid Hybridization; D012343:RNA, Transfer / Q000235:genetics* / Q000302:isolation & purification; D012469:Salivary Glands / Q000648:ultrastructure; D014443:Tyrosine / Q000235:genetics*
D014443:Tyrosine; D012343:RNA, Transfer