Properties of Hg- and Cd-spores of Bacillus megaterium QM B1551.
Hg- and Cd-spores of Bacillus megaterium QM B1551 were produced in Schaeffer's medium containing mercuric chloride and cadmium chloride respectively. Metals were added to the medium at 9 hr of incubation (Stage V) to give a final concentration of 50 microM. It was found by electron microscopic and biochemical studies that the coats of both Hg- and Cd-spores were thinner than those of control spores. Of the total Hg and Cd in the spores, 77% of the Hg and 63% of the Cd were detected in the spore coats. Hg- and Cd-spores were less resistant to heat and more sensitive to germinants than control spores. Other properties of Hg- and Cd-spores were similar to those of control spores. These results suggest that the spore coat has some relationship to the heat resistance and germinability of spores.
D001410:Bacillus megaterium / Q000502:physiology*; D002104:Cadmium / Q000032:analysis* / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D008628:Mercury / Q000032:analysis* / Q000494:pharmacology; D013171:Spores, Bacterial / Q000032:analysis*; D013997:Time Factors
D003470:Culture Media; D002104:Cadmium; D008628:Mercury