Calcium-dependent protease in neuroblastoma cells.
Extracts of neuroblastoma cells contained calcium-dependent proteolytic activity. This activity was accounted for by two distinct proteases. These proteases were separated by ion-exchange chromatography. Although each was totally dependent on calcium, the calcium requirements of the enzymes were different; one enzyme required approximately 40 microM Ca2+ for half-maximal activity and the other enzyme required approximately 150 microM Ca2+ for half-maximal activity.
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002413:Cations, Divalent; D002460:Cell Line; D004533:Egtazic Acid / Q000494:pharmacology; D007700:Kinetics; D009447:Neuroblastoma / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D002413:Cations, Divalent; D013439:Sulfhydryl Reagents; D004533:Egtazic Acid; D010447:Peptide Hydrolases; D002118:Calcium