Brain uptake of pipecolic acid, amino acids, amines following intracarotid injection in the mouse.
The uptake of pipecolic acid by the mouse brain was compared to that of several amino acids and amines, following an injection of a double-labeled mixture into the carotid artery. In general, BUI (brain uptake index) values were lower in the mouse than those previously reported in the rat. The only exception was proline. Lysine, a precursor of pipecolic acid biosynthesis in brain, showed a higher BUI than pipecolic acid. The BUI of D,L-[3H]pipecolic acid was found to be 3.39 (at 0.114 mM). This was saturable between a concentration of 0.114 and 3.44 mM. Kinetic analysis suggests the presence of two kinds of transport systems. Substances structurally related to pipecolic acid, such as nipecotic acid, isonipecotic acid, L-proline, and piperidine show a significant inhibitory effect. Amont the amino acids tested, only GABA showed an inhibitory effect. Data are reported which, when considered with other findings (5), present evidence that pipecolic acid is (1) synthesized both in vitro and in vivo in the mouse brain, (2) actively transported in vivo into the brain, and (3) taken up in vitro by synaptosomal preparations.
D000588:Amines / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D001921:Brain / Q000378:metabolism*; D002250:Carbon Radioisotopes; D007700:Kinetics; D008239:Lysine / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010875:Pipecolic Acids / Q000378:metabolism*; D011392:Proline / Q000378:metabolism; D051381:Rats; D014316:Tritium
D000588:Amines; D000596:Amino Acids; D002250:Carbon Radioisotopes; D010875:Pipecolic Acids; D014316:Tritium; D011392:Proline; C031345:pipecolic acid; D008239:Lysine