[GABA fluophore formation due to ninhydrin reaction in the octanolic milieu. Spectrofluorometric investigation. 2nd information (author's transl)].
As a result of the reaction of ninhydrin with gamma-aminobutyric acid and glutamic acid in the octanolic milieu, a fluorescent product, presumably a copper-II-chelate-complex, was formed. Partial reaction steps were investigated spectrofluorometrically. The excitation and emmission spectra resulting from the reaction between ninhydrin and glutamic acid, ninhydrin and gamma-aminobutyric acid, and ninhydrin, glutamic acid and gamma-aminobutyric acid were measured. Possible consequences are discussed with respect to fluorescence histochemical procedures.
D003300:Copper; D005971:Glutamates / Q000032:analysis; D007202:Indicators and Reagents; D009555:Ninhydrin; D000442:Octanols; D013050:Spectrometry, Fluorescence / Q000379:methods; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D005971:Glutamates; D007202:Indicators and Reagents; D000442:Octanols; D005680:gamma-Aminobutyric Acid; D003300:Copper; D009555:Ninhydrin
gamma-Aminobuttersäure- (GABA-) Fluorescenz bei der Ninhydrin-Reaktion im octanolischen Milieu. Spektrofluorometrische Untersuchung. 2. Mitteilung.