The acidic macromolecules in rabbit cortical bone tissue.
Rabbit cortical bone powder was extracted with EDTA solutions at neutral pH and the soluble constituents fractionated by DEAE-cellulose chromatography. The acidic fractions obtained were further investigated by gel chromatography and chemical analysis. Two classes of proteoglycans were present; one containing chondroitin sulphate and the other containing material resembling keratan sulphate mixed with a smaller amount of chondroitin sulphate. Sialoglycoproteins were detected by specific chemical introduction of tritium label into the sialic acid residues of the acidic glycoprotein fraction. Following sodium dodecyl sulphate-polyacrylamide gel electrophoresis and fluorography, the presence of numerous distinct sialoglycoprotein components was demonstrated.
D000818:Animals; D001842:Bone and Bones / Q000032:analysis*; D002809:Chondroitin Sulfates / Q000032:analysis; D002845:Chromatography; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D006025:Glycosaminoglycans / Q000032:analysis*; D007632:Keratan Sulfate / Q000032:analysis; D046911:Macromolecular Substances; D011509:Proteoglycans / Q000032:analysis*; D011817:Rabbits; D012795:Sialoglycoproteins / Q000032:analysis*
D006025:Glycosaminoglycans; D046911:Macromolecular Substances; D011509:Proteoglycans; D012795:Sialoglycoproteins; D002809:Chondroitin Sulfates; D007632:Keratan Sulfate; D004492:Edetic Acid