Incorporation of [14C]-leucine into complement in experimental infection of rats in malnourished stages.
The complement biosynthesis was examined in the first complement response, that is at the stage of elevated complement level 3 days after Staphylococcus aureus infection, since this elevation might play a role in host defence before antibody response. [14C]-leucine was used as a labeled amino acid to examine the de novo synthesis of the complement. In the first complement response, the malnourished rats red with 0.5% protein, both in infection and noninfection groups, showed a significantly higher specificity in newly synthesized complement activity than did the rats in the well-nourished group fed with 18% protein. The infected rats showed much higher rates of de novo synthesis of complement activity than noninfected rats and this effect was more predominant in the malnourished group.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism; D003165:Complement System Proteins / Q000096:biosynthesis* / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008297:Male; D011485:Protein Binding / Q000187:drug effects; D011488:Protein Deficiency / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D013203:Staphylococcal Infections / Q000276:immunology*
D000936:Antigen-Antibody Complex; D003165:Complement System Proteins; D004492:Edetic Acid; D007930:Leucine