Specific reversal of cytolytic T lymphocyte--target cell interaction.
A functional assay is described that measures the reversal of specific cytolytic T cell (CTL)-target cell binding. Binding of 51Cr-labeled P815 cells was stable in suspension but could be readily reversed by the addition of unlabeled P815 cells. The reversal of CTL-tumor cell and CTL-spleen cell binding was H-2 specific; only cells of the same H-2 type as the bound target cell could induce reversal. In all cases, tumor cells were substantially more efficient than spleen cells in inducing specific reversal.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D012515:Mast-Cell Sarcoma / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D012513:Sarcoma, Experimental / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D008274:Magnesium; D002118:Calcium