A simple procedure for isolation and purification of A-type staphylococcus enterotoxin.
A new procedure is presented for the isolation and purification of A-type staphylococcus enterotoxin. Homogeneous enterotoxin preparation was obtained by purification in 2 phases. In radial double agar-gel immunodiffusion the smallest precipitating dose of the isolated and purified enterotoxin was found to be 1.4-0.7 micrograms protein and 0.4-0.1 micrograms nitrogen. In cat experiments the dose giving a positive reaction was 2 micrograms protein or 0.5 micrograms nitrogen calculated for kg body weight.
D000645:Ammonium Sulfate; D000818:Animals; D002415:Cats; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D004589:Electrophoresis, Disc; D004768:Enterotoxins / Q000302:isolation & purification* / Q000633:toxicity; D005779:Immunodiffusion; D008722:Methods
D004768:Enterotoxins; C026066:enterotoxin A, Staphylococcal; D000645:Ammonium Sulfate