Isolation of hormonal proteins and antibodies by affinity chromatography.
The versatile technique of biospecific affinity chromatography has found application in the purification as well as isolation of many hormones and their antibodies. Hormones can be effectively concentrated from very dilute solutions such as urine or other fluids including culture media. The technique may be expected to have a big impact in the isolation of hormone receptors. Its limitations are not unique to the above macromolecules but extend to the general method itself, the most important being the development of stable immobilized ligands and efficient but still mild solvent systems for elution. The use of dilute ammonia solutions at low temperatures has been quite successful for displacing either the hormone or the antibody.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification* / Q000652:urine; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D004285:Dogs; D005399:Fishes; D000882:Haplorhini; D006728:Hormones / Q000302:isolation & purification* / Q000652:urine; D006801:Humans; D051381:Rats; D012756:Sheep; D013552:Swine
D000906:Antibodies; D006728:Hormones