title: Establishment of regional differences in brush border enzymatic activities during the development of fetal mouse small intestine.
abstract: In order to study the establishment of regional differences in brush border enzymic activities during the development of fetal mouse small intestine we have followed (1) the differentiation of microvilli by morphometry, and (2) the developmental pattern of three brush border enzymes (lactase, glucoamylase and alkaline phosphatase). From day 16 to day 19 of gestation, the height of duodenal microvilli increases 2.4 times on the absorptive cells located near the tip of the villi. During the same period in the upper half of the duodenal villi, the number of microvilli per square micrometer rises by a factor of 2.4 and the microvillous surface area increases by a factor of 5.2. The differentiation of ileal microvilli follows a similar pattern but they are always shorter and less numerous than those of the duodenum. Lactase activity appears at 18 days of gestation; the other two brush border enzymes are first detected at 16 days of gestation. Afterwards all three enzyme activities increase rapidly and a decreasing gradient of activity is established from the proximal to the distal segment of the small intestine. Hence, the structural development of the microvilli and the appearance of brush border enzyme activities occur simultaneously and a proximo-distal gradient is already established at 16 days of gestation.
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology*; D005696:Galactosidases / Q000378:metabolism*; D005865:Gestational Age; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism*; D005959:Glucosidases / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000196:embryology; D007421:Intestine, Small / Q000196:embryology* / Q000201:enzymology; D051379:Mice; D008813:Mice, Inbred ICR; D008871:Microvilli / Q000201:enzymology* / Q000648:ultrastructure; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D000469:Alkaline Phosphatase; D005696:Galactosidases; D005959:Glucosidases; D001616:beta-Galactosidase; D005087:Glucan 1,4-alpha-Glucosidase