Terminal stages of complement hemolysis: technical comment.
In studying terminal lytic events of sensitized erythrocytes which have reacted with all 9 components of complement, we agree in general with the conclusions of Boyle and Borsos [1] that the cellular intermediate passes through the stages E*bound, E*inserted, and E*doomed. However, we differ with these workers on the influence of 3 variables on the terminal reactions. These variables are: time of hemolysis; temperature of E* preparation; and the use of EDTA to manipulate E*.
D000818:Animals; D003185:Complement C8 / Q000378:metabolism; D003186:Complement C9 / Q000378:metabolism; D003165:Complement System Proteins / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000378:metabolism; D006168:Guinea Pigs; D006461:Hemolysis; D007158:Immunologic Techniques; D013696:Temperature; D013997:Time Factors
D003185:Complement C8; D003186:Complement C9; D003165:Complement System Proteins; D004492:Edetic Acid