Use of monoclonal antibody to increase sensitivity and specificity in quantitative immunodiffusion assays.
A mouse monoclonal antibody has been produced which recognizes human liver gamma-cystathionase, Radioiodination of the monoclonal antibody facilitated its use in combination with non-specific precipitating rabbit antisera in classical immunodiffusion assays. The technique may have broad applicability in the detection and quantitation of rare antigens which are difficult to purify but easily recognizable in immunodiffusion assays. It may also be used for the initial detection of monoclonal antibodies to unique antigens of interest.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D003542:Cystathionine gamma-Lyase / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D008099:Liver / Q000201:enzymology; D011234:Precipitins / Q000276:immunology; D011817:Rabbits
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007106:Immune Sera; D011234:Precipitins; D003542:Cystathionine gamma-Lyase