Simple method for quantitation of viable mycoplasmas.
A rapid, simple, and inexpensive method for quantitation of viable mycoplasmas is described. Serial dilutions were made in sterile microtiter plates with standard microtiter equipment. The results were multiplied by a factor of 2.38 to obtain colony-forming units comparable to those obtained with the laborious glass pipette-tube dilution method.
D000362:Agar; D001431:Bacteriological Techniques; D003470:Culture Media; D009174:Mycoplasma / Q000302:isolation & purification*; D009177:Mycoplasma pneumoniae / Q000302:isolation & purification*
D003470:Culture Media; D000362:Agar