Improved liquid chromatographic assay for serum fluorouracil concentrations in the presence of ftorafur.
An improved liquid chromatographic assay for serum ftorafur and fluorouracil is shown to be routine, sensitive, and reproducible using 200 micro l of serum. Dilute ammonium acetate buffer at pH 10.2 is used for solubilization of the evaporated ethyl acetate extract for injection into the liquid chromatograph. A stability study indicated little or no in vitro formation of fluorouracil from ftorafur under the conditions described. Low serum fluorouracil levels were found after administration of therapeutic doses of ftorafur.
D002853:Chromatography, Liquid / Q000379:methods; D004355:Drug Stability; D004356:Drug Storage; D005472:Fluorouracil / Q000031:analogs & derivatives* / Q000097:blood* / Q000627:therapeutic use; D005770:Gastrointestinal Neoplasms / Q000188:drug therapy; D006801:Humans; D005641:Tegafur / Q000097:blood* / Q000627:therapeutic use
D005641:Tegafur; D005472:Fluorouracil