title: The hexa- and pentapeptide extension of proalbumin. II. Processing of specific antibodies against the synthetic hexapeptide.
abstract: The chemically synthesized proalbumin hexapeptide was coupled to rabbit albumin with carbodiimide. Subsequently rabbits were immunized by subcutaneous injection of the conjugate. After 5 weeks the rabbits had developed antibodies against the hexapeptide, which could be detected by immunodiffusion. A sensitive enzyme-linked immunosorbent assay was developed. Using the hexapeptide and other very similar peptides as antigens, a high specificity of the antibodies against the proalbumin hexapeptide was found.
mesh_terms: D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D004797:Enzyme-Linked Immunosorbent Assay; D005779:Immunodiffusion; D009842:Oligopeptides / Q000138:chemical synthesis / Q000276:immunology*; D011228:Prealbumin / Q000276:immunology*; D011817:Rabbits; D012709:Serum Albumin / Q000276:immunology*
chemical_list: D000906:Antibodies; D000936:Antigen-Antibody Complex; D009842:Oligopeptides; D011228:Prealbumin; D012709:Serum Albumin