title: Cellular localization of the Mn2+-dependent adenylyl cyclase in the human testis.
abstract: An examination of the activity of the Mn2+-dependent adenylyl cyclase (AC) in fine needle biopsies from human testes was made. Simultaneously the DNA distribution patterns in suspensions of testicular cells derived from the same patients have been determined. The DNA distribution patterns were estimated by microflow fluorimetry (MFF) after straining with fluorochrome (ethidium bromide). Thus, AC activity could be assessed and correlated with the relative number of haploid (1C =  spermatids), diploid (2C = spermatogonia and testicular somatic cells), and tetraploid (4C = primary spermatocytes) cells. Testicular Mn2+-dependent AC activities varied between 0 and 8.4 pmol cyclic adenosine monophosphate (cAMP)/mg protein/min and were highly correlated with the contents of haploid (1C) germ cells (spermatids) (r = 0.62, p less than 0.01). There was no correlation between Mn2+-dependent AC activity and diploid or tetraploid cells. This indicates that the Mn2+-dependent AC activity in the human testis, like in the rat and mouse, may be exclusively localized to haploid germ cells. An inverse correlation between plasma FSH and Mn2+-dependent AC activities indicated reduced inhibin secretion in situations where the Sertoli cells did not maintain the testicular germ cell production.
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D001707:Biopsy, Needle; D004247:DNA / Q000378:metabolism; D005434:Flow Cytometry; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D008297:Male; D008345:Manganese / Q000378:metabolism*; D009845:Oligospermia / Q000473:pathology; D013737:Testis / Q000201:enzymology*; D014018:Tissue Distribution
chemical_list: D008345:Manganese; D005640:Follicle Stimulating Hormone; D004247:DNA; D000262:Adenylyl Cyclases