Immunocytochemical evidence for the specific localization of aldose reductase in rat Sertoli cells.
Evidence that the enzyme aldose reductase (AR) is specifically located in Sertoli cells is presented by means of an established immunocytochemical technique and with a variety of approaches. By staining tissue sections, the enzyme was shown to be present in Sertoli cells at birth and the intensity of the immunocytochemical stain increased by 5 days of age to that found in the testes of older rats. By means of enzyme dispersion of mature testes; the culture of enriched Sertoli cell preparations from the testes of immature rats; and the collection of newly released testicular spermatozoa in rete testis fluid, it was shown that immunoreactive AR was not present in any testicular cell type except the Sertoli cell. The significance of the specific localization in Sertoli cells of a principal enzyme concerned in the sorbitol or polyol pathway for the conversion of aldose sugars to their corresponding ketoses is discussed.
D000375:Aging; D000449:Aldehyde Reductase / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D003593:Cytoplasm / Q000201:enzymology; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D008297:Male; D051381:Rats; D012708:Sertoli Cells / Q000201:enzymology*; D013091:Spermatogenesis; D013094:Spermatozoa / Q000201:enzymology; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism*; D013737:Testis / Q000166:cytology
D013401:Sugar Alcohol Dehydrogenases; D000449:Aldehyde Reductase