title: Interrelationship of glycogen metabolism and lactose synthesis in mammary epithelial cells of mice.
abstract: Glycogen metabolism in mammary epithelial cells was investigated (i) by studying the conversion of glucose into glycogen and other cellular products in these cells from virgin, pregnant and lactating mice and (ii) by assaying the enzymes directly involved with glycogen metabolism. We find that: (1) mammary epithelial cells synthesized glycogen at rates up to over 60% that of the whole gland; (2) the rate of this synthesis was modulated greatly during the reproductive cycle, reaching a peak in late pregnancy and decreasing rapidly at parturition, when abundant synthesis of lactose was initiated; (3) glycogen synthase and phosphorylase activities reflected this modulation in glycogen metabolism; (4) lactose synthesis reached a plateau during late pregnancy, even though lactose synthase is reported to increase in the mouse mammary gland at this time. We propose that glycogen synthesis restricts lactose synthesis during late pregnancy by competing successfully for the shared UDP-glucose pool. The physiological advantage of glycogen accumulation during late pregnancy is discussed.
mesh_terms: D000818:Animals; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000096:biosynthesis*; D006006:Glycogen Synthase / Q000378:metabolism; D007774:Lactation; D007785:Lactose / Q000096:biosynthesis*; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D006005:Phosphorylases / Q000378:metabolism; D011247:Pregnancy
chemical_list: D006003:Glycogen; D006005:Phosphorylases; D006006:Glycogen Synthase; D005947:Glucose; D007785:Lactose