[The in vitro effects of purified protein derivative (PPD) on the lymphocytes and phagocytes from patients with active pulmonary tuberculosis and healthy tuberculin reactors].
In a previous report, we found no difference in surface markers of lymphocytes and phagocytes between patients with active pulmonary tuberculosis and healthy tuberculin reactors. In this communication, 45 cases of active pulmonary tuberculosis and 21 cases of healthy tuberculin reactors were studied with regard to the changes of surface markers of lymphocytes and the expression of Fc and complement receptors on phagocytes after in vitro incubation with PPD. After PPD stimulation, the monocytes with Fc and complement receptors tended to increase in tuberculin-positive but not in tuberculin-negative patients or healthy tuberculin reactors, and the active T lymphocytes were markedly increased in normal individuals but not in patients. Thus, the failure of lymphocytes from patients to respond to in vitro PPD activation with increased active T lymphocytes may account for the establishment of tuberculous infection after exposure to mycobacteria and the inability of the monocytes from tuberculin-negative patients to be activated by PPD in vitro correlates with negative skin test in vivo.
D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology; D011951:Receptors, Complement / Q000032:analysis; D011961:Receptors, Fc / Q000032:analysis; D014373:Tuberculin / Q000276:immunology*; D014374:Tuberculin Test; D014397:Tuberculosis, Pulmonary / Q000276:immunology*
D011951:Receptors, Complement; D011961:Receptors, Fc; D014373:Tuberculin