title: ICR-191 and ethyl methanesulfonate induced mutagenesis at the immunoglobulin locus in the Y5606 cultured myeloma cell line.
abstract: The Y5606 mouse tumor synthesizing an IgG3, lambda immunoglobulin (Ig) was adapted to continuous growth in tissue culture. The spontaneous mutation rate at the Ig locus (approximately 3 X 10(-5)/cell/generation) in this cell line was found to be less than that in other cultured mouse myeloma lines. Treatment with either ICR-191 or ethyl methanesulfonate (EMS) increased the mutation rate approx. 100-fold. Spontaneous and ICR-191 induced mutants were synthetic variants that is they synthesized either heavy (H) or light (L) chains alone instead of the H and L chains synthesized by the parent. Following EMS treatment assembly variants which were synthesizing structurally altered H chains were isolated in addition to synthetic variants. The assembly variants appear to be a unique consequence of EMS mutagenesis.
mesh_terms: D000585:Aminacrine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D005796:Genes; D007074:Immunoglobulin G / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D051379:Mice; D009101:Multiple Myeloma; D009154:Mutation; D009374:Neoplasms, Experimental / Q000235:genetics; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology
chemical_list: D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D009588:Nitrogen Mustard Compounds; D000585:Aminacrine; C004294:acridine half-mustard