Reactivation and immunological studies on a Japanese variant of red cell carbonic anhydrase I, CA I Hiroshima.
1. Under certain conditions, CA I HIR-1 (86 Asp leads to Gly) was inactivated in Gnd . HCl more rapidly than normal CA I. 2. After denaturation in 5.0 M Gnd . HCl, CA I HIR-1 was reactivated more slowly than normal CA I. 3. CA I HIR-1 was precipitated by purified anti-CA I antibodies, under certain conditions, more rapidly than normal CA I.
D000941:Antigens; D002256:Carbonic Anhydrases / Q000276:immunology / Q000378:metabolism*; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007564:Japan; D009154:Mutation; D011489:Protein Denaturation
D000941:Antigens; D002256:Carbonic Anhydrases