Enzyme-linked immunosorbent assay for detection of antibody to Pseudomonas aeruginosa and measurement of antibody titer in horse serum.
Enzyme-linked immunosorbent assay (ELISA) was used for detection of immunoglobulin (Ig) M and IgG antibodies against a serologically common antigen (original endotoxin protein), protease, and elastase of Pseudomonas aeruginosa. The P aeruginosa antibody in horse sera was measured, using ELISA. Horseradish peroxidase-labeled rabbit anti-horse IgM and IgG antibodies were used for enzyme-labeled antibody conjugate. 5-Aminosalicylic acid and H2O2 were used for substrate. Sera collected from a vaccinated horse, a newborn foal, and 72 healthy racehorses were investigated for antibodies against P aeruginosa by ELISA and passive hemagglutination procedure. Changes in IgM and IgG antibody titers with vaccination were clear by ELISA. In the newborn foal, significant amounts of IgM and IgG antibodies from colostrum were present on the 1st day after birth. It was shown by ELISA that the level of antibodies in the newborn decreased initially and then increased. Some antibodies against original endotoxin protein, protease, and elastase of P aeruginosa were detected in almost all the healthy racehorses investigated.
D000818:Animals; D000831:Animals, Newborn; D000907:Antibodies, Bacterial / Q000032:analysis*; D004731:Endotoxins / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests / Q000662:veterinary; D006734:Horse Diseases / Q000276:immunology; D006736:Horses / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D010196:Pancreatic Elastase / Q000276:immunology; D010447:Peptide Hydrolases / Q000276:immunology; D011552:Pseudomonas Infections / Q000276:immunology / Q000662:veterinary; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary
D000907:Antibodies, Bacterial; D004731:Endotoxins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010447:Peptide Hydrolases; D010196:Pancreatic Elastase