Rapid micromethod for preparation of enzyme-antibody conjugates.
A rapid method is described for labeling antibody with alkaline phosphatase by one-step glutaraldehyde linkage. The method involves the centrifugation of a small volume of an enzyme and antibody mixture through a minicolumn packed with hydrated Sephadex. This procedure rapidly removes ammonium sulfate and glutaraldehyde from the enzyme-antibody mixture and results in the efficient recovery of conjugated antibody without significant dilution.
D000469:Alkaline Phosphatase; D000645:Ammonium Sulfate; D000906:Antibodies; D002498:Centrifugation; D055598:Chemical Phenomena; D002621:Chemistry; D005976:Glutaral; D007124:Immunoenzyme Techniques
D000906:Antibodies; D000469:Alkaline Phosphatase; D000645:Ammonium Sulfate; D005976:Glutaral