title: Immunosorbent assays for diagnosis of Crimean-Congo hemorrhagic fever (CCHF).
abstract: Utilization of direct and indirect enzyme-linked immunosorbent assay (ELISA) and solid-phase radioimmunoassay (SPRIA) for the diagnosis of Crimean-Congo hemorrhagic fever (CCHF) allows the detection of low amounts of infectious virus (2 log LD50) or inactivated antigen and antibody to CCHF within 5-6 hours. These methods were shown to be more sensitive, specific, rapid, and reproducible than the complement-fixation test, immunofluorescence, hemagglutination, or radial diffusion in gel. The experimental design of ELISA and SPRIA developed for CCHF may be used successfully for the detection of the other members of the Bunyaviridae family.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000276:immunology; D006479:Hemorrhagic Fever, Crimean / Q000175:diagnosis* / Q000276:immunology; D007074:Immunoglobulin G; D007158:Immunologic Techniques; D051379:Mice; D011863:Radioimmunoassay; D051381:Rats
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G