Influence of drugs on the bilirubin UDP-glucuronyltransferase activity and the concentration of Y and Z acceptor proteins in rat liver.
The aim of this paper is to report on the influence of phenobarbital, the combination of phenobarbital/nikethamide, the racemate and (+) isomer of methylphenobarbital on the activity of bilirubin UDP-glucuronyltransferase and the concentration of intrahepatic Y and Z protein in rats. The activity of bilirubin DP-glucuronyltransferase increased after the treatment in all groups. Pretreatment with phenobarbital/nikethamide resulted in the highest enzyme activity, using wet weight as reference. (+) Methylphenobarbital isomer as well as the racemate showed effects similar to those of phenobarbital. The concentration of hepatic Y acceptor protein increased in all pretreated groups by about 30%. The drugs did not increase the concentration of Z protein. The (+) isomer of methylphenobarbital seems better suited as an inductive drug in the prophylaxis of hyperbilirubinemia and in the treatment of nonconjugated hyperbilirubinemia than phenobarbital or phenobarbital/nikethamide since it has no sedative effect.
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D003593:Cytoplasm / Q000187:drug effects* / Q000378:metabolism; D004338:Drug Combinations; D050556:Fatty Acid-Binding Proteins; D014453:Glucuronosyltransferase / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008618:Mephobarbital / Q000494:pharmacology; D009552:Nikethamide / Q000008:administration & dosage; D010634:Phenobarbital / Q000008:administration & dosage; D051381:Rats; D011919:Rats, Inbred Strains; D013237:Stereoisomerism; D013268:Stimulation, Chemical
D002352:Carrier Proteins; D004338:Drug Combinations; D050556:Fatty Acid-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; C033503:Y acceptor protein, rat; D009552:Nikethamide; D008618:Mephobarbital; D014453:Glucuronosyltransferase; D010634:Phenobarbital