Stimulatory effect of prostaglandin E1 on thyroliberin-induced alpha-melanotropin release from perifused neuro-intermediate lobes of frog pituitary gland.
A possible direct effect of prostaglandins on alpha-melanotropin (alpha-MSH) release at the level of the intermediate lobe of the frog pituitary was investigated in vitro using a perifusion system technique. The effect of prostaglandins was studied on both spontaneous and TRH-stimulated alpha-MSH secretion. No significant effect of PGE1, PGE2, PGF1 alpha or PGF2 alpha on basal release of alpha-MSH could be detected. Indomethacin did not alter the alpha-MSH release induced by TRH. Conversely a significant increase in TRH-induced alpha-MSH secretion was observed in the presence of 1 x 10(-6) M PGE1. This magnifying effect was directly related to the concentration of TRH for doses ranging from 1 x 10(-8) M to 1 x 10(-6) M.
D000818:Animals; D004305:Dose-Response Relationship, Drug; D007213:Indomethacin / Q000494:pharmacology; D009074:Melanocyte-Stimulating Hormones / Q000378:metabolism*; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism*; D011458:Prostaglandins E / Q000494:pharmacology*; D011460:Prostaglandins F / Q000494:pharmacology; D011895:Rana ridibunda; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology*
D011458:Prostaglandins E; D011460:Prostaglandins F; D013973:Thyrotropin-Releasing Hormone; D009074:Melanocyte-Stimulating Hormones; D007213:Indomethacin