title: Non-decalcified barium sulfate-adsorbed plasma. A potentially useful reagent for studying blood clotting, platelets or complement.
abstract: Plasma was prepared by rapid centrifugation of native blood followed by adsorption of vitamin K-dependent clotting factors with BaSO4. Compared to fresh citrated plasma, BaSO4-treated plasma contained about 80% of factor V and factor VII and the same concentration of fibrinopeptide A. Assays were also carried out after overnight incubation of citrated plasma and BaSO4-adsorbed plasma with and without added citrate and compared to assays of fresh citrated plasma. Factor V decreased to about 25% in both citrated samples, factor VIII decreased to 45% in both samples of BaSO4-treated plasma, and fibrinopeptide A did not change. Thus loss of factor V activity depended on reduction in divalent cation concentration whereas loss of factor VIII activity may have resulted from effects of early traces of thrombin.
mesh_terms: D000327:Adsorption; D001466:Barium Sulfate; D001777:Blood Coagulation; D005165:Factor V / Q000032:analysis; D005169:Factor VIII / Q000032:analysis; D005344:Fibrinopeptide A / Q000032:analysis; D006801:Humans; D007202:Indicators and Reagents
chemical_list: D007202:Indicators and Reagents; D005344:Fibrinopeptide A; D001466:Barium Sulfate; D005165:Factor V; D005169:Factor VIII