title: [beta-1,3-1,4-glucanase in sporeforming microorganisms. III. Substrate specificity and action patterns of some Bacillus-beta-glucan-hydrolases (author's transl)].
abstract: Comparative investigations were carried out concerning substrate specificity and action patterns of seven Bacillus-endo-beta-glucanases produced by the species, B. subtilis, B. macerans, B. amyloliquefaciens, B. circulans, B. laterosporus, B. pumilus and B. polymyxa. All enzymes with the exception of beta-glucanase from B. macerans hydrolyze lichenan and barley-beta-glucan only and were without action on laminaran and CM-cellulose. It was suggested that hydrolysis products of beta-glucanase produced by B. macerans were markedly different from the products of the other enzymes. We conclude that B. macerans enzyme, which cleaves laminaran and beta-1,3-1,4-glucans, represents "laminarinase" type (1-3-beta-D-glucan glucanohydrolase, E.C. 3.2.1.6). On the other hand the glucanases produced by the other Bacillus strains belong to "licheninases" 1-3,1-4-beta-D-glucan glucanohydrolases, E.C. 3.2.1.73).
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D001412:Bacillus subtilis / Q000201:enzymology*; D002266:Carboxymethylcellulose Sodium / Q000378:metabolism; D005936:Glucans / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D005936:Glucans; D011134:Polysaccharides; C008247:laminaran; D006026:Glycoside Hydrolases; C028020:licheninase; D002266:Carboxymethylcellulose Sodium; C015968:lichenin
vernacular_title: beta-1,3-1,4-Glucanase in sporenbildenden Mikroorganismen. III. Substratspezifität und Wirkungsweise einiger Bacillus-beta-Glucan-Hydrolasen.