The effect of aphidicolin on the rate of DNA replication and unscheduled DNA synthesis of Bloom syndrome and normal fibroblasts.
Ultraviolet radiation induced more unscheduled DNA synthesis (UDS) in ten Bloom syndrome (BS) fibroblast strains than in control cells, but this difference could be suppressed by aphidicolin treatment in at least nine BS strains. Aphidicolin, 1 and 5 micrograms/ml, were required to inhibit by 30% the UDS of BS and control cells respectively, but the DNA replication of BS cells did not prove abnormally sensitive to such an inhibitor. These findings are discussed in relation to current knowledge of the action of aphidicolin and hypotheses of the metabolic defect in BS.
D016590:Aphidicolin; D001816:Bloom Syndrome / Q000235:genetics*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000528:radiation effects; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors*; D004260:DNA Repair; D004261:DNA Replication / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D019384:Nucleic Acid Synthesis Inhibitors; D014466:Ultraviolet Rays
D004224:Diterpenes; D019384:Nucleic Acid Synthesis Inhibitors; D016590:Aphidicolin; D004247:DNA; D004257:DNA Polymerase II