[Total synthesis of human big gastrin I. Revised primary structure (author's transl)].
The synthesis of the tetratriacontapeptide amide corresponding to the revised structure of human big gastrin I is described. The fully protected peptide derivative was obtained by assembly in sequence order of the suitably protected fragments [1--9], [10--14] and [15--34] via the dicyclohexylcarbodiimide/N-hydroxysuccinimide and azide method, respectively. Upon removal of the protecting groups by exposure to trifluoroacetic acid and purification of the resulting crude product by chromatographic methods, human big gastrin I was obtained in satisfactory yields and at a high degree of purity. The identical immunological crossreactivities of natural and synthetic human big gastrin I using anti-porcine big gastrin I antiserum strongly supports the correctness of the newly proposed primary structure of this member of the gastrin family.
D000595:Amino Acid Sequence; D005755:Gastrins / Q000138:chemical synthesis*; D006801:Humans; D005779:Immunodiffusion; D008722:Methods; D011498:Protein Precursors; D014269:Trifluoroacetic Acid
D005755:Gastrins; D011498:Protein Precursors; C013706:big gastrin; D014269:Trifluoroacetic Acid
Totalsynthese des Human-Big-Gastrins I. Revidierte Primärstruktur.