[The evidence of antibodies against motor endplates of the skeletal muscle in myasthenia gravis with the double immune-fluorescence technique (author's transl)].
Studies were undertaken on sera of 66 patients for detecting antibodies to motor endplates using a double immunofluorescence method according to Sondag-Tschroots et al. Rat tongue was used as antigenic substrate. Strong positive results at a serum dilution of 1: 20 were found only in patients with myasthenia gravis (15 out of 42 patients approximately equal to 35.7%). Titres between 1: 20 and 1: 1,280 occurred. 3 of 8 patients with chronic active hepatitis and with circulating antibodies to smooth muscle and 2 of 4 patients with autoimmune disorders and weakly positive antibodies in their 1: 20 diluted serum. The used rat tongue as antigenic substrate seems to be more suitable than diaphragm for detecting of such and other antibodies.
D001323:Autoantibodies / Q000032:analysis*; D001327:Autoimmune Diseases / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006505:Hepatitis / Q000276:immunology; D006801:Humans; D009045:Motor Endplate / Q000276:immunology*; D009157:Myasthenia Gravis / Q000276:immunology*; D009469:Neuromuscular Junction / Q000276:immunology*
D001323:Autoantibodies
Doppelimmunfluoreszenzoptischer Nachweis von Antikörpern gegen motorische Endplatten des Skeletmuskels bei Myasthenia gravis.