A new mitogenic D-galactosephilic lectin isolated from seeds of the coral-tree Erythrina corallodendron. Comparison with Glycine max (soybean) and Pseudomonas aeruginosa lectins.
The lectin of Erythrina corallodendron (Caesalpiniaceae) seeds was purified by heating, ammonium sulfate fractionation, and affinity chromatography on acid-treated Sepharose. The purified lectin is similar to the soybean lectin in being a glycoprotein of molecular weight around 110 000 - 120 000 and having D-galactosephilic activity. This lectin, like the soybean and Pseudomonas aeruginosa lectins, binds to D-galactosamine, N-acetyl-D-galactosamine, alpha- and beta-galactosides as well as to D-galactose. Like these lectins it absorbs onto either untreated or enzyme (papain or neuraminidase) treated human red blood cells, but exhibits a considerable mitogenic activity towards human lymphocytes (predominantly T cells) only after their treatment with neuraminidase. This mitogenic stimulation of lymphocytes is inhibited by D-galactose and its derivatives. Despite the great similarity between them, the E. corallodendron, soybean, and Pseudomonas lectins differ in regard to the intensity of their agglutinating activity towards erythrocytes obtained from different animals and human donors of diverse ABO blood groups. This phenomenon may be attributed to the difference in the affinities of the three lectins to the various D-galactose derivatives and to their molecular properties.
D000116:Acetylgalactosamine / Q000494:pharmacology; D004895:Erythrina; D004903:Erythrocyte Aggregation / Q000187:drug effects; D005688:Galactosamine / Q000494:pharmacology; D005690:Galactose / Q000494:pharmacology*; D005697:Galactosides / Q000494:pharmacology; D006801:Humans; D037102:Lectins / Q000302:isolation & purification* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D009439:Neuraminidase; D010206:Papain; D037121:Plant Lectins; D010946:Plants, Medicinal; D011550:Pseudomonas aeruginosa / Q000032:analysis*; D012639:Seeds; D013025:Glycine max / Q000032:analysis
D005697:Galactosides; D037102:Lectins; D037121:Plant Lectins; D005688:Galactosamine; D009439:Neuraminidase; D010206:Papain; D000116:Acetylgalactosamine; D005690:Galactose