Formation of 8,11,12-trihydroxyeicosatrienoic acid by a rat lung high-speed supernatant fraction.
An enzyme was found in the high-speed (100 000 x g) supernatant fraction of a rat lung homogenate which catalysed the conversion of arachidonic acid into 8,11,12-trihydroxyeicosatrienoic acid. The isomeric 8,9,12-triol was not detected. The structure of the isolated product was confirmed by mass spectrometric analysis of the methyl ester t-butyldimethylsilyl ether derivative. These results indicate that formation of both positional isomers is carried out by separate enzymes, the distributions of which are not restricted to platelets.
D015126:8,11,14-Eicosatrienoic Acid / Q000031:analogs & derivatives / Q000096:biosynthesis*; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D007536:Isomerism; D008168:Lung / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D051381:Rats
D001095:Arachidonic Acids; D005231:Fatty Acids, Unsaturated; D016718:Arachidonic Acid; C019121:8,11,12-trihydroxy-5,9,14-eicosatrienoic acid; D015126:8,11,14-Eicosatrienoic Acid