Peanut agglutinin: a marker for normal and leukaemic cells of the monocyte lineage.
The potential of peanut agglutinin (PNA) as a monocyte lineage marker and a diagnostic tool was investigated. Peripheral blood mononuclear cells (MNC) from normal adults, and 20 patients with acute non-lymphocytic leukaemia (ANLL), as well as five human cell lines were analysed for fluorescein isothiocyanate (FITC)-PNA binding using the fluorescence activated cell sorter (FACS). Monocytes and monoblasts, which were positive for non-specific esterase activity, bound FITC-PNA. In addition, FITC-PNA was bound by a population of cells not defined by cytochemical criteria which were probably precursors to monoblasts. The classical myeloid blast cell did not bind FITC-PNA. ANLL classification is discussed in the light of these results. FITC-PNA clearly binds to cells of the monocyte lineage, and has potential as an adjunct to current cytochemical and morphological criteria in classification.
D002454:Cell Differentiation; D002460:Cell Line; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D005690:Galactose / Q000494:pharmacology; D006801:Humans; D037102:Lectins / Q000378:metabolism; D007938:Leukemia / Q000175:diagnosis*; D007951:Leukemia, Myeloid / Q000175:diagnosis; D008214:Lymphocytes / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism*; D019887:Peanut Agglutinin; D010587:Phagocytosis; D013861:Thiocyanates / Q000378:metabolism
D005452:Fluoresceins; D005456:Fluorescent Dyes; D037102:Lectins; D019887:Peanut Agglutinin; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate; D005690:Galactose