[Fluorescence histochemical investigations on the carbohydrate metabolism of the rat brain (author's transl)].
The Regio frontalis posterior of the motor cortex of adult rats was investigated by means of the fluorescence-PAS-reaction. Using various Schiff-type-reagents - substituted with acriflavine, rhodamine B, rhodamine 6G, safranine T - a fluorescent reaction product has been found in different amounts in the pyramidal neurons and in other cellular elements. After application of acriflavine different degrees of PAS-reactivity could be demonstrated in various cortices. Dexamethasone affected an increase of the fluorescent reaction product in individual pyramidal neurons. Ideas on the possible reaction mechanism after application of various Schiff-type-reagents have been proposed.
D000167:Acriflavine; D000818:Animals; D050260:Carbohydrate Metabolism; D003907:Dexamethasone / Q000494:pharmacology; D006651:Histocytochemistry / Q000379:methods*; D007202:Indicators and Reagents; D008297:Male; D008856:Microscopy, Fluorescence; D009044:Motor Cortex / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D010503:Periodic Acid-Schiff Reaction; D010619:Phenazines; D051381:Rats; D012235:Rhodamines
D007202:Indicators and Reagents; D010619:Phenazines; D012235:Rhodamines; D000167:Acriflavine; D003907:Dexamethasone; C009195:safranine T
Fluorescenzhistochemische Untersuchungen zum Carbohydratstoffwechsel des Gehirns der Ratte.