Protonation of methotrexate bound to the catalytic site of dihydrofolate from Lactobacillus casei.
D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007780:Lacticaseibacillus casei / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D008727:Methotrexate / Q000378:metabolism*; D011485:Protein Binding; D013053:Spectrophotometry; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism*
D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate