Active synthesis of glycosaminoglycans by liver parenchymal cell in primary culture.
Rat liver parenchymal cells were evaluated after 2 days of primary culture for their ability to synthesize and accumulate heparan sulfate as the major component and low-sulfated chondroitin sulfate, dermatan sulfate, chondroitin sulfate and hyaluronic acid as the minor ones. The newly synthesized glycosaminoglycans secreted into the medium were different from those remaining with and/or the cell layer. Low-sulfated chondroitin 4-sulfate, a major glycosaminoglycan in blood, was synthesized in the order of 320 microgram/liver per day, more than 90% of which was secreted into the medium with 16 h and 40% of the glycan secreted was degraded during that time. On the other hand, heparan sulfate, the major glycosaminoglycan synthesized by the parenchymal cells, was mainly distributed in the cell layer. After 8 days of culture, the synthesis of glycosaminoglycans by the cells increased markedly, especially dermatan sulfate, chondroitin sulfate, chondroitin sulfate and hyaluronic acid.
D000818:Animals; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000096:biosynthesis; D002810:Chondroitinases and Chondroitin Lyases / Q000378:metabolism; D003124:Colorimetry; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006497:Heparan Sulfate / Q000096:biosynthesis; D006820:Hyaluronic Acid / Q000096:biosynthesis; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats
D002809:Chondroitin Sulfates; D002810:Chondroitinases and Chondroitin Lyases; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006820:Hyaluronic Acid