In vitro translation of mRNA for yeast citrate synthase.
The citrate synthase of yeast was purified to homogeneity and shown to have a subunit molecular weight of 52,000. Antibodies were prepared to it in rabbits. Translation of total yeast mRNA using a reticulocyte lysate showed that [3H]leucine was incorporated into a protein precipitable by rabbit anti-yeast citrate synthase with a molecular weight of about 54,000. No incorporation of [35S]methionine into an anti-citrate synthase precipitable protein could be detected in a similar experiment. In vivo labeling of cells with 35SO4 did not result in the labeling of citrate synthase.
D000818:Animals; D002474:Cell-Free System; D002950:Citrate (si)-Synthase / Q000235:genetics* / Q000302:isolation & purification; D005779:Immunodiffusion; D007652:Oxo-Acid-Lyases / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D012333:RNA, Messenger; D002950:Citrate (si)-Synthase; D007652:Oxo-Acid-Lyases