Safety and immunogenicity of high molecular weight polysaccharide vaccine from immunotype 1 Pseudomonas aeruginosa.
The safety and immunogenicity of a high molecular weight polysaccharide from immunotype 1 Pseudomonas aeruginosa were tested in a dose response fashion in adult volunteers. The vaccine lacked toxicity and pyrogenicity for experimental animals. Doses of 50, 75, 150, or 250 microgram were given to groups of individuals as a single dose subcutaneous injection. Doses of 150 and 250 microgram were associated with a significant rise in binding and opsonic antibody at 2 wk postimmunization. Titers remained unchanged for up to 6 mo. The vaccine was almost devoid of toxicity, eliciting no more than a slightly sore and tender arm at the site of injection. High molecular weight polysaccharide antigen appears to induce a good immune response following vaccination that is effective in mediating opsonophagocytic killing of live P. aeruginosa organisms.
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology* / Q000592:standards* / Q000633:toxicity; D006168:Guinea Pigs; D006737:Horseshoe Crabs / Q000276:immunology; D006801:Humans; D008970:Molecular Weight; D009895:Opsonin Proteins / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000276:immunology / Q000592:standards; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D012703:Serotyping
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D009895:Opsonin Proteins; D011135:Polysaccharides, Bacterial