Substrate specificity and mode of action of the cellulases from the thermophilic fungus Thermoascus aurantiacus.
The substrate specificities of three cellulases and a beta-glucosidase purified from Thermoascus aurantiacus were examined. All three cellulases partially degraded native cellulose. Cellulase I, but not cellulase II and cellulase III, readily hydrolyzed the mixed beta-1,3; beta-1,6-polysaccharides such as carboxymethyl-pachyman, yeast glucan and laminarin. Both cellulase I and the beta-glucosidase degraded xylan, and it is proposed that the xylanase activity is an inherent feature of these two enzymes. Lichenin (beta-1,4; beta-1,3) was degraded by all three cellulases. Cellulase II cannot degrade carboxymethyl-cellulose, and with filter paper as substrate the end product was cellobiose, which indicates that cellulase II is an exo-beta-1,4-glucan cellobiosylhydrolase. Degradation of cellulose (filter paper) can be catalysed independently by each of the three cellulases; there was no synergistic effect between any of the cellulases, and cellobiose was the principal product of degradation. The mode of action of one cellulase (cellulase III) was examined by using reduced cellulodextrins. The central linkages of the cellulodextrins were the preferred points of cleavage, which, with the rapid decrease in viscosity of carboxymethyl-cellulose, confirmed that cellulase III was an endocellulase. The rate of hydrolysis increased with chain length of the reduced cellulodextrins, and these kinetic data indicated that the specificity region of cellulase III was five or six glucose units in length.
D001203:Ascomycota / Q000201:enzymology*; D002266:Carboxymethylcellulose Sodium / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism*; D002850:Chromatography, Gel; D003912:Dextrins / Q000378:metabolism; D006868:Hydrolysis; D011134:Polysaccharides / Q000378:metabolism; D013379:Substrate Specificity; D014783:Viscosity; D001617:beta-Glucosidase / Q000378:metabolism
D003912:Dextrins; D011134:Polysaccharides; D001617:beta-Glucosidase; D002480:Cellulase; D002266:Carboxymethylcellulose Sodium