title: The branching and linear portions of poly(adenosine diphosphate ribose) have the same alpha(1 leads to 2) ribose-ribose linkage.
abstract: The structure of the branching site of poly(ADP-ribose) was determined as O-alpha-D-ribofuranosyl-(1"' leads to 2")-O-alpha-D-ribofuranosyl-(1" leads to 2')-adenosine-5',5",5"'-tris(phosphate) by gas chromatography, mass spectrometry, and 1H-NMR measurements. Thus the structures of all the ribose-ribose linkages known in poly(ADP-ribose) are uniformly alpha(1 leads to 2)glycosidic bond. This indicates that branching ADP-ribosylation and elongating ADP-ribosylation of poly(ADP-ribose) synthesis are catalyzed by similar alpha(1 leads to 2)-specific ADP-ribosyl transferases or the same enzyme. Poly(ADP-ribose) glycohydrolase, which specifically hydrolyzes the ribose-ribose bonds of poly(ADP-ribose), also cleaves the ribose-ribose-ribose bonds at the site of branching.
mesh_terms: D002849:Chromatography, Gas; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009702:Nucleoside Diphosphate Sugars; D011064:Poly Adenosine Diphosphate Ribose; D012266:Ribose
chemical_list: D009702:Nucleoside Diphosphate Sugars; D011064:Poly Adenosine Diphosphate Ribose; D012266:Ribose