Production of cryoprecipitate of intermediate purity in a closed system thaw-siphon process.
The thaw-siphon procedure for routine production of cryoprecipitate is described briefly, and also its extension to a reproducible procedure for production of cryoprecipitates of intermediate purity. For the latter, the average yield of factor VIII is 60% of that in the prefrozen plasma and approximately 30% is present in the supernatant plasma. The product contains 0.53 units of VIII: C per mg of total protein (of which about 70% is clottable). The mean value of the ratio VIII: C/VIII: RAG is about 0.7. The yield and purity of the product compare favourably with those reported for intermediate purity factor VIII concentrates produced by large-scale plasma fractionation laboratories. The procedure offers an alternative pathway to the production of factor VIII concentrate of intermediate purity and can be operated in any blood bank with a minimum of equipment.
D000941:Antigens / Q000302:isolation & purification; D001793:Blood Preservation / Q000379:methods; D001798:Blood Proteins / Q000032:analysis; D011232:Chemical Precipitation; D003450:Cryoglobulins / Q000302:isolation & purification*; D005169:Factor VIII / Q000276:immunology / Q000302:isolation & purification*; D005615:Freezing; D006801:Humans; D014841:von Willebrand Factor
D000941:Antigens; D001798:Blood Proteins; D003450:Cryoglobulins; D014841:von Willebrand Factor; D005169:Factor VIII