[Immunofluorescence histochemical detection of carbonic anhydrase in bovine stomach mucosa and kidney (author's transl)].
An antiserum against carbonic anhydrase from bovine erythrocytes was produced and tested on paraffin sections of bovine rennet-bag and kidney with an indirect immunofluorescence technique. A specific immunofluorescence was found in the parietal cells, mucoid surface, and foveolar cells of rennet-bag mucosa. In the kidney, there was a positive reaction mainly in the distal nephron segments as the loops of Henle and the distal tubules and also in the collecting tubes. The glomeruli and proximal tubules reacted negatively. The specificity of the found distribution of immunofluorescence was confirmed by some control reactions and was also demonstrated by partial congruence with results obtained by the cobalt method. The demonstrated enzyme activities are compared with results from the literature.
D000818:Animals; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002417:Cattle; D004912:Erythrocytes / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D005753:Gastric Mucosa / Q000166:cytology / Q000201:enzymology*; D007668:Kidney / Q000201:enzymology*
D002256:Carbonic Anhydrases
Immunofluorescenzhistochemischer Nachweis von Carboanhydrase in der Magenmucosa und Niere des Rindes.