Induction of cellulose in Schizophyllum commune: thiocellobiose as a new inducer.
Several mono-, di, tetra-, and polysaccharides were screened for their ability to induced cellulase production by the tetrapolar hymenomycete Schizophyllum commune. Out of 21 carbohydrates screened, 4 (thiocellobiose, carboxymethylcellulose, cellobiose, and xylan) induced all three enzymes tested (carboxymethylcellulase, beta-glucosidase, and xylanase). The inducing effect increased with rising concentrations of the inducers up to a certain value, beyond which there was either a leveling off or a decrease of the enzymatic activities. The most powerful inducer, thiocellobiose, showed the highest activity at 0.5 mM. Cellobiose, carboxymethylcellulose, and xylan showed their highest activities at 1 mM and 1%, respectively. Surprisingly, sophorose did not enhance enzyme production. The enzymatic activities were monitored over a period of 24 h. Thiocelloboise elicited a response immediately after incubation, but with all other inducers there was a latency period before their effect could be measured. High-performance liquid chromatography showed no hydrolysis of thiocellobiose when incubated in the presence of S. commune extracellular enzymes.
D000363:Agaricales / Q000201:enzymology*; D002266:Carboxymethylcellulose Sodium; D002475:Cellobiose; D002480:Cellulase / Q000096:biosynthesis*; D002482:Cellulose; D004790:Enzyme Induction; D006026:Glycoside Hydrolases / Q000096:biosynthesis; D012567:Schizophyllum / Q000201:enzymology*; D013865:Thioglycosides / Q000378:metabolism; D043325:Xylan Endo-1,3-beta-Xylosidase; D014990:Xylans; D001617:beta-Glucosidase / Q000096:biosynthesis
D013865:Thioglycosides; D014990:Xylans; D002475:Cellobiose; C033514:thiocellobiose; D002482:Cellulose; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase; D002266:Carboxymethylcellulose Sodium