title: Changes in galactosyltransferase activity in chick pectoral muscle during embryonic development.
abstract: The two major vertebrate galactosyltransferases have been investigated in developing chick muscle in ovo and in vitro, and in cultured chick fibroblasts. The two enzymes were UDP-galactose-N-acetylglucosamine galactosyltransferase (galactosyltransferase I) and UDP-galactose-N-acetylgalactosamine galactosyltransferase (galactosyltransferase II). Both activities fell during muscle development in ovo. Galactosyltransferase I activity was constant from day 7 to day 16, after which it declined 5-fold, whereas galactosyltransferase II activity fell markedly from day 9 to 13 and 16 to 20, displaying an overall 8-fold decrease. In primary muscle cultures, galactosyltransferase I activity fell slightly during 7 days in culture, whereas galactosyltransferase II increased 2-fold during the same period. No significant change in activity of either galactosyltransferase was observed during intercellular recognition and fusion. Analysis of muscle cultures treated with cytosine arabinoside and of fibroblast cultures revealed that the majority of galactosyltransferase I activity in primary muscle cultures is associated with fibroblasts, whereas the majority of galactosyltransferase II activity is muscle-associated. The addition of 5-bromodeoxyuridine to primary muscle cultures resulted in a 3-fold rise in activities of both transferases.
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002478:Cells, Cultured; D002642:Chick Embryo; D003561:Cytarabine / Q000494:pharmacology; D005347:Fibroblasts / Q000201:enzymology; D005700:Galactosyltransferases / Q000378:metabolism*; D009132:Muscles / Q000187:drug effects / Q000196:embryology / Q000201:enzymology*; D009237:N-Acetyllactosamine Synthase / Q000378:metabolism
chemical_list: D003561:Cytarabine; D005700:Galactosyltransferases; C032545:UDPgalactose-N-acetylgalactosamine galactosyltransferase; D009237:N-Acetyllactosamine Synthase; D001973:Bromodeoxyuridine