[Plasmid DNA transduction in Bacillus subtilis].
Transduction of Bacillus subtilis pUB110 plasmid by AR9 phage is described. Some aspects of this process are studied. Plasmid transduction depended on multiplicity of infection similar to cases of chromosomal markers transduction, though optimal multiplicity of infection was achieved using low number of phage particles. No cotransduction of plasmid and chromosomal markers was demonstrated. The transduction frequencies of plasmid and chromosomal markers increased after UV irradiation of phage suspensions within the range of definite doses.
D001412:Bacillus subtilis / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000528:radiation effects; D004269:DNA, Bacterial / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000528:radiation effects; D005819:Genetic Markers / Q000528:radiation effects; D010957:Plasmids / Q000528:radiation effects; D014161:Transduction, Genetic / Q000528:radiation effects; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D005819:Genetic Markers
Transduktsiia plazmidnoĭ DNK u Bacillus subtilis.