Effect of gonadal hormones on the incorporation of [3H]lysine into proteins of human fetal brain.
Samples were obtained from the hypothalamus, temporal lobe and frontal cortex of brains from human fetuses aged 14-22 weeks. Slices were prepared and incubated with estradiol, testosterone or dihydrotestosterone, and the incorporation of [3H]lysine into protein was compared with that of untreated control tissue. Cytosol and crude nuclear subcellular fractions from each area were made and resolution of specific proteins was carried out using a polyacrylamide gel system. Steroid hormones had no detectable effect on total protein synthesis, but incorporation of [3H]lysine into certain low molecular weight proteins of the hypothalamus increased significantly in the presence of estradiol and testosterone. Dihydrotestosterone was inactive. The temporal lobe and frontal cortex were unaffected by the steroid hormones.
D001921:Brain / Q000187:drug effects*; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005625:Frontal Lobe / Q000378:metabolism; D005865:Gestational Age; D006801:Humans; D007031:Hypothalamus / Q000378:metabolism; D008239:Lysine / Q000378:metabolism*; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D011247:Pregnancy; D013702:Temporal Lobe / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology*
D009419:Nerve Tissue Proteins; D013196:Dihydrotestosterone; D013739:Testosterone; D004958:Estradiol; D008239:Lysine