Genetic control of the immune response to haemoglobin. III. Variant A beta (bm12) but not Ae (D2.GD) Ia polypeptides alter immune responsiveness towards the alpha -subunit of human haemoglobin.
Mice bearing the I-A subregion mutation bm12 were immunized and challenged with the alpha -subunit of human adult haemoglobin. Under conditions in which parental B6/Kh mice respond, B6.C-H-2bm12 mice are inhibited nearly 100% in their ability to respond to challenge to the alpha-chain of haemoglobin. D2.GD mice which express a variant Ae (E beta) polypeptide of the I-E subregion can respond as well as B10.D2 mice to both subunits (alpha- and beta-) of haemoglobin. These observations as well as other genetic mapping data confirm the I-A mapping of alpha -chain-specific Ir genes and extend the genetic fine mapping to the A beta gene within the I-A subregion or a combinatorial Ia determinant generated by an interaction of A alpha and A beta. In addition they implicate the Ia.8 specificity in determining immune responsiveness to alpha -chain determinants.
D000818:Animals; D000917:Antibody Formation; D002874:Chromosome Mapping; D005260:Female; D005802:Genes, MHC Class II; D006454:Hemoglobins / Q000276:immunology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D011498:Protein Precursors / Q000276:immunology*
D006454:Hemoglobins; D011498:Protein Precursors