Detection of a monoclonal human myeloma protein of IgM and IgG class by carp anti-idiotypic sera.
In the Patient St. with a Morbus Waldenström macroglobulinemia a double paraproteinemia could be detected. Besides the IgM myeloma protein an IgG myeloma protein was identified during the clinical course. A strong cross reactivity between the IgM and the IgG myeloma proteins was shown using anti-idiotypic antisera. This is an indirect indication for a common precursor cell clone of the IgM- and IgG-myeloma protein producing cells. The anti-idiotypic antisera were made in carp. The high specificity of these antisera could be confirmed by inhibition assays. The double paraproteinemia has been proved to be convenient model for testing the idiotypic specificity of anti-Id antisera of carp.
D000818:Animals; D002347:Carps / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006801:Humans; D007106:Immune Sera; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000032:analysis*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007075:Immunoglobulin M / Q000032:analysis*; D009194:Myeloma Proteins / Q000032:analysis*; D010265:Paraproteinemias / Q000276:immunology; D008258:Waldenstrom Macroglobulinemia / Q000276:immunology
D007106:Immune Sera; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M; D009194:Myeloma Proteins