Chromatographic fractionation of anti-sickling agents in Fagara xanthoxyloides.
Fractionation of an aqueous extract of root bark from Fagara xanthoxyloides by column chromatography on DEAE-A-50, utilizing an elution gradient of pH 7.5-5.0, yielded five fractions. All fractions reversed metabisulfite-induced sickling in vitro of erythrocytes homozygous for hemoglobin S. Only the fraction containing the least anti-sickling activity also contained alkaloids. Thin-layer chromatography on silica gel and aluminum oxide revealed further molecular heterogeneity within two of the fractions.
D000470:Alkaloids / Q000302:isolation & purification* / Q000494:pharmacology; D000986:Antisickling Agents / Q000302:isolation & purification* / Q000494:pharmacology; D002848:Chromatography, DEAE-Cellulose; D004336:Drug Antagonism; D004912:Erythrocytes / Q000187:drug effects; D004913:Erythrocytes, Abnormal / Q000187:drug effects; D006451:Hemoglobin, Sickle; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010936:Plant Extracts / Q000032:analysis; D010946:Plants, Medicinal / Q000032:analysis*; D013447:Sulfites / Q000494:pharmacology
D000470:Alkaloids; D000986:Antisickling Agents; D006451:Hemoglobin, Sickle; D010936:Plant Extracts; D013447:Sulfites; C009279:sodium bisulfite