[Immunocytochemical detection in Stannius corpuscles of the eel (Anguilla anguilla L.) of a hormone similar to the mammalian parathyroid hormone].
In eels the parathyrin of the corpuscles of Stannius (PCS), mammalian parathyroid-like hormone, has been localized in the cytoplasm of all the cells in the corpuscles. This detection was done by indirect immunofluorescence with an antiserum anti 1-84 bovine parathormone. The specificity of the reaction was demonstrated by inhibition of the coloration obtained with the 1-84 bovine parathormone and the active fragment 1-34 of human parathormone. Variations of the cellular localization of the PCS or a complete depletion of the hormonal content were observed in eels made hypercalcaemic by calcium overloading.
D000811:Anguilla / Q000502:physiology*; D000818:Animals; D002118:Calcium; D004702:Endocrine Glands / Q000166:cytology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006728:Hormones / Q000032:analysis*; D006934:Hypercalcemia / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010281:Parathyroid Hormone / Q000032:analysis*
D006728:Hormones; D010281:Parathyroid Hormone; D002118:Calcium
Détection immunocytochimique dans les corpuscules de Stannius de l'anguille (Anguilla anguilla L.) d'une hormone proche de l'hormone parathyroïdienne mammalienne.