Glutaraldehyde-treated carrier erythrocytes for organ targeting of methotrexate in dogs.
Chemotherapeutic doses of methotrexate (MTX) were encapsulated in as little as 1 ml of canine carrier erythrocytes. Carrier erythrocytes prepared by a dialysis technique contained more than 3.0 mg of MTX/ml of cells. In vivo pharmacokinetic studies indicated that most of the drug leaked from the circulating MTX-loaded erythrocytes. In dogs, glutaraldehyde treatment of MTX-loaded erythrocytes prevented drug leakage and targeted more than 50% of the drug to the liver.
D000818:Animals; D004285:Dogs / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology*; D005976:Glutaral / Q000494:pharmacology; D008727:Methotrexate / Q000008:administration & dosage* / Q000097:blood; D014677:Pharmaceutical Vehicles
D014677:Pharmaceutical Vehicles; D005976:Glutaral; D008727:Methotrexate