In vivo therapy of a murine B cell tumor (BCL1) using antibody-ricin A chain immunotoxins.
Prolonged remissions were induced in mice bearing advanced BCL1 tumors by the combined approach of nonspecific cytoreductive therapy and administration of a tumor-reactive immunotoxin. Thus, the vast majority of the tumor cells (approximately 95%) were first killed by nonspecific cytoreductive therapy using total lymphoid irradiation (TLI) and splenectomy. The residual tumor cells were then eliminated by intravenous administration of an anti-delta immunotoxin. In three of four experiments, all animals treated in the above fashion appeared tumor free 12-16 wk later. In one experiment, blood cells from the mice in remission were transferred to normal BALB/c recipients, and the latter animals have not developed detectable tumor for the 6 mo of observation. Because 1-10 adoptively transferred BCL1 cells will cause tumor in normal BALB/c mice by 12 wk, the inability to transfer tumor to recipients might indicate that the donor animals were tumor free. In the remainder of the animals treated with the tumor-reactive immunotoxin there was a substantial remission in all animals, but the disease eventually reappeared. In contrast, all mice treated with the control immunotoxin or antibody alone relapsed significantly earlier (3-4 wk after splenectomy).
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000008:administration & dosage; D000992:Antitoxins / Q000008:administration & dosage / Q000627:therapeutic use*; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects; D005455:Fluorescent Antibody Technique; D007138:Immunoglobulin delta-Chains / Q000276:immunology; D037102:Lectins / Q000276:immunology*; D007942:Leukemia, Experimental / Q000188:drug therapy* / Q000532:radiotherapy; D007958:Leukocyte Count; D008221:Lymphoid Tissue / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D037121:Plant Lectins; D010940:Plant Proteins / Q000276:immunology*; D011817:Rabbits; D013156:Splenectomy
D000888:Antibodies, Anti-Idiotypic; D000992:Antitoxins; D007138:Immunoglobulin delta-Chains; D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; C017707:Ricinus communis agglutinin-1