EM autoradiographic evidence that DNA synthesis occurs at recombination nodules during meiosis in Drosophila melanogaster females.
Serial section electron microscopic autoradiography was used to examine the relationship between recombination nodules and 3H-thymidine incorporation during pachytene in Drosophila melanogaster females. For both ellipsoidal and spherical recombination nodules, the number of nodules that are associated with an autoradiographic grain is higher than that expected by chance; this observation is consistent with the hypotheses that recombination involves DNA synthesis and that recombination nodules are the sites of meiotic recombination. Moreover, general DNA replication (S-phase) and synapsis (synaptonemal complex formation) were found to be temporally distinct events, contrary to previous reports; Drosophila females therefore are not exceptional in this regard.
D000818:Animals; D001345:Autoradiography; D002875:Chromosomes / Q000648:ultrastructure*; D004261:DNA Replication; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D008540:Meiosis; D008854:Microscopy, Electron; D011995:Recombination, Genetic; D013997:Time Factors