False reactions in radioimmunoassay for viral hepatitis B markers in patients suffering from coagulation disorders.
Simultaneous occurrence of hepatitis B surface antigen (HBsAg) and antibodies to HBsAg (anti-HBs) has been frequently described in hemophiliac patients. During our working procedures, this phenomenon was also observed. Interference of coagulation disorders with radioimmunoassay (RIA) used in the detection of viral hepatitis B markers was studied. It was shown that, in vivo or in vitro, correction of coagulation disorders led to the disappearance of false positive reactions and, thus, simultaneous presence of HBsAg and anti-HBs never occurred. Confirmatory tests which are usually recommended to control RIA were reviewed and it found that only a completed coagulation of serum by thrombin addition, before performing the test, gave specific results.
D000914:Antibodies, Viral / Q000032:analysis*; D001778:Blood Coagulation Disorders / Q000276:immunology*; D005164:Factor IX / Q000627:therapeutic use; D005169:Factor VIII / Q000627:therapeutic use; D005189:False Positive Reactions; D006386:Hemagglutination Tests; D006467:Hemophilia A / Q000276:immunology / Q000628:therapy; D002836:Hemophilia B / Q000276:immunology / Q000628:therapy; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D009500:Neutralization Tests; D011863:Radioimmunoassay; D013917:Thrombin / Q000494:pharmacology; D013997:Time Factors
D000914:Antibodies, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D005169:Factor VIII; D005164:Factor IX; D013917:Thrombin