Human seminal lectin. II. Properties of a lectin like factor in the human seminal plasma.
A lectin like haemagglutinin (Human Seminal Lectin - HSL) is present in human seminal plasma. Partial purification and characterization of HSL has been achieved. HSL was shown to be almost completely inactivated at 100 degree C within 15 min. HSL was stable within a pH range of 5 to 10 for one hour, was resistant to trypsin, mercaptoethanol and dithiothreitol and has a molecular weight of about 57 000 daltons and an iso-electric point of about 6.8. HSL activity did not depend on either the incubation temperature or on the availability of free divalent cations such as Ca++,Mg++,or Mn++.
D002850:Chromatography, Gel; D004492:Edetic Acid / Q000494:pharmacology; D006388:Hemagglutinins / Q000302:isolation & purification*; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008297:Male; D008970:Molecular Weight; D012661:Semen / Q000737:chemistry*
D006388:Hemagglutinins; D004492:Edetic Acid