title: Purification and properties of L-3-glycerophosphate dehydrogenase from pig brain mitochondria.
abstract: L-3-Glycerophosphate dehydrogenase (EC 1.1.99.5) was purified from pig brain mitochondria by extraction with deoxycholate, ion-exchange chromatography and (NH4)2SO4 fractionation in cholate, and preparative isoelectric focusing in Triton X-100. Sodium dodecyl sulphate/polyacrylamide gel electrophoresis shows that the purified enzyme consists of a single subunit of mol.wt. 75 000. The enzyme contains non-covalently bound FAD and low concentrations of iron and acid labile sulphide. No substrate reducible e.p.r. signals were detected. The conditions of purification, particularly the isoelectric focusing step, lead to considerable loss of FAD and possibly iron-sulphur centres. It is therefore not possible to decide with certainty whether the enzyme is a flavoprotein or a ferroflavoprotein. The enzyme catalyses the oxidation of L-3-glycerophosphate by a variety of electron acceptors, including ubiquinone analogues. A number if compounds known to inhibit ubiquinone oxidoreduction by other enzymes of the respiratory chain failed to inhibit L-3-glycerophosphate dehydrogenase, except at very high concentrations.
mesh_terms: D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose; D005415:Flavins / Q000032:analysis; D005993:Glycerolphosphate Dehydrogenase / Q000302:isolation & purification*; D007501:Iron / Q000032:analysis; D007525:Isoelectric Focusing; D008055:Lipids / Q000032:analysis; D008928:Mitochondria / Q000201:enzymology*; D013440:Sulfides / Q000032:analysis; D013552:Swine
chemical_list: D005415:Flavins; D008055:Lipids; D013440:Sulfides; D007501:Iron; D005993:Glycerolphosphate Dehydrogenase