16S ribosomal RNA of Escherichia coli contains a N2-methylguanosine at 27 nucleotides from the 3' end.
The 49 nucleotides fragment derived from the 3' end of 16S rRNA by cloacin DF13, is not cleaved by ribonuclease T1 at a guanosine residue tha is present at 27 nucleotides from the 3' terminus (position 115 in 16S rRNA). Analysis of the isolated nucleotide indicates that it is a modified G residue. In vivo labeling with (3H)methionine shows that this G is methylated and co-chromatography with markers reveals that it is N2-methylguanosine.
D001483:Base Sequence; D002989:Cloacin; D004926:Escherichia coli / Q000737:chemistry*; D006151:Guanosine / Q000031:analogs & derivatives* / Q000032:analysis; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal; D006163:Ribonuclease T1; D014316:Tritium
D012335:RNA, Ribosomal; D014316:Tritium; D006151:Guanosine; C016578:7-methylguanosine; D002989:Cloacin; D006163:Ribonuclease T1