Quantitation of platelet-associated IgG by radial immunodiffusion.
Platelet-associated IgG (PAIgG) was measured by a simple radial immunodiffusion technique using washed solubilized platelets and commercially available immunoplates. Subjects with normal platelet counts had PAIgG levels of 1.5--7.0 fg/platelet. Subjects with idiopathic immune thrombocytopenic purpura (ITP) had levels ranging from 5.7 to 70.5 fg/platelet. All patients with recurrent ITP and 85% of patients with acute ITP had elevated PAIgg. Elevated PAIgG was also found in 17% of patients with recovered ITP, 40% of patients with SLE and thrombocytopenia, 57% of patients with thrombocytopenia occurring during the course of septicemia, and 100% of patients with IgG myeloma in whom the serum IgG level was clearly elevated, regardless of the platelet count. The results are similar to reports that used more complex techniques.
D001792:Blood Platelets / Q000276:immunology*; D002951:Citrates / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D007074:Immunoglobulin G; D010976:Platelet Count; D013921:Thrombocytopenia / Q000276:immunology
D002951:Citrates; D007074:Immunoglobulin G; D004492:Edetic Acid; D005947:Glucose