Alkaline phosphatase activity as a membrane marker for activated B cells.
Alkaline phosphatase activity was assayed by a microtiter assay (with p-nitrophenylphosphate used as substrate) in the plasma membrane of mouse spleen cells activated in vitro by the B cell mitogen LPS and the T cell-dependent B cell mitogen, PWM. No activity was detected in spleen cells cultured in the presence of the T cell mitogens PHA and Con A. This alkaline phosphatase activity was detected in the blast-enriched 30 to 40% fraction of discontinuous Percoll gradients in LPS-treated cultures, and the enzymatic activity assayed was susceptible to inhibition by the alkaline phosphatase inhibitors EDTA and L-phenylalanine. These data support the idea that the membrane alkaline phosphatase activity could be used as a marker for activated B cells.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001402:B-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology / Q000276:immunology; D004492:Edetic Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008213:Lymphocyte Activation; D008223:Lymphoma / Q000201:enzymology / Q000276:immunology; D051379:Mice; D008806:Mice, Inbred AKR; D010649:Phenylalanine / Q000494:pharmacology; D010954:Plasmacytoma / Q000201:enzymology / Q000276:immunology
D010649:Phenylalanine; D004492:Edetic Acid; D000469:Alkaline Phosphatase