[Studies on the biochemical alterations of cartilage proteoglycan in antigen induced arthritis of rabbit (author's transl)].
Proteoglycan alterations were studied in articular cartilage obtained from control and antigen induced arthritis of the rabbits. Agarose/polyacrylamide-gel electrophoretic patterns of proteoglycan revealed heterogeneity in each experiment. In the cartilage of the antigen induced arthritis the following changes were demonstrated at the early stage; 1) an increase in the non-aggregated proteoglycan content, 2) a decrease in the size of the proteoglycan, 3) a decrease in the ability to interact with hyaluronic acid, 4) a constancy in the length of the glycosaminoglycan chains, 5) a decrease in hexuronate/protein, galactosamine/glucosamine and galactosamine/amino acid ratio, and 6) a decrease in serine and threonine content of the core protein. At the late stage the following changes were most pronounced; 1) a recovery in the size of the proteoglycan, 2) a marked decrease in the ability to interact with hyaluronic acid, 3) an increase in galactosamine/glucosamine and a gradual decrease in glucosamine/amino acid ratio, and 4) an increase in serine and glycine and a decrease in half cysteine and methionine content of the core protein. The results indicate that the smaller size of the proteoglycan from articular cartilage was due to degradation of the core protein at the early stage. But there seems to be some changes of proteoglycan synthesis at the late stage.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001168:Arthritis / Q000378:metabolism*; D001169:Arthritis, Experimental / Q000378:metabolism*; D002358:Cartilage, Articular / Q000378:metabolism*; D002809:Chondroitin Sulfates / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006025:Glycosaminoglycans / Q000032:analysis; D011509:Proteoglycans / Q000378:metabolism*; D011817:Rabbits
D000596:Amino Acids; D006025:Glycosaminoglycans; D011509:Proteoglycans; D002809:Chondroitin Sulfates