title: Molecular cloning of genes for cellobiose utilization and their expression in Escherichia coli.
abstract: The genes for cellobiose utilization in Escherichia adecarboxylata were cloned by using recombinant deoxyribonucleic acid techniques and transferred to Escherichia coli. Preliminary analysis of the beta-glucosidase activity expressed in these host cells indicated that the enzyme is membrane bound and required magnesium ions, phosphate ions, and heat-stable, non-dialyzable factors from the bacterial cytoplasm.
mesh_terms: D002462:Cell Membrane / Q000201:enzymology; D002475:Cellobiose / Q000378:metabolism*; D003001:Cloning, Molecular; D004187:Disaccharides / Q000378:metabolism*; D004925:Escherichia / Q000201:enzymology / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005959:Glucosidases / Q000235:genetics*; D008274:Magnesium / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D001617:beta-Glucosidase / Q000235:genetics* / Q000378:metabolism
chemical_list: D004187:Disaccharides; D010710:Phosphates; D002475:Cellobiose; D005959:Glucosidases; D001617:beta-Glucosidase; D008274:Magnesium