Divalent cation ionophores stimulate resorption and inhibit DNA synthesis in cultured fetal rat bone.
Two divalent cation ionophores, A23187 and Ionomycin, which are selective for calcium, stimulated the resorption of fetal rat long bones in organ culture at 0.1 to 1 micromolar but not at higher concentrations. Both agents inhibited DNA synthesis at concentrations that stimulated resorption. These results might explain the differences in ionophore effects on bone previously reported, and they imply that cell replication is not required for osteoclast formation in fetal rat long bone cultures.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001862:Bone Resorption / Q000187:drug effects*; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002132:Calcium Radioisotopes; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication / Q000187:drug effects*; D004987:Ethers / Q000494:pharmacology; D005333:Fetus; D015759:Ionomycin; D007476:Ionophores / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D010281:Parathyroid Hormone / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D002132:Calcium Radioisotopes; D004987:Ethers; D007476:Ionophores; D010281:Parathyroid Hormone; D000001:Calcimycin; D015759:Ionomycin; D004247:DNA; D002118:Calcium