title: Effect of ammonia on glucose oxidation by isolated rat adipocytes.
abstract: The effect of ammonium and acetate salts on glucose metabolism in isolated rat adipocytes was studied. Ammonia in low concentration (1-10 mM) was found to inhibit insulin-stimulated CO2 production. Acetate had no effect on this system except at high concentrations (100 mM), at which it stimulated CO2 production. Ammonium and acetate salts had an anti-lipolytic effect at high concentration (100 mM) but no effect on lipolysis at lower concentrations. The observation that trace amounts of salts can affect a biological assay system suggests that other biological assay system may also be altered by contaminating buffer salts from protein isolation steps.
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000641:Ammonia / Q000494:pharmacology*; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology*; D050356:Lipid Metabolism; D009994:Osmolar Concentration; D051381:Rats
chemical_list: D007328:Insulin; D002245:Carbon Dioxide; D000641:Ammonia; D005947:Glucose