Indirect hemagglutination test that uses glutaraldehyde-fixed sheep erythrocytes sensitized with extract antigens for detection of Pasteurella antibody.
Glutaraldehyde-fixed sheep erythrocytes (GA-SRBC) were used in the indirect hemagglutination test for the detection of Pasteurella antibody. GA-SRBC were stable for at least 6 months. Heat extract or potassium thiocyanate extract antigens of Pasteurella strains could be adsorbed onto GA-SRBC or tanned GA-SRBC, respectively. The indirect hemagglutination test reaction was capsular group specific with heat extract antigen-sensitized GA-SRBC but not potassium thiocyanate extract antigen-sensitized tanned GA-SRBC.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000302:isolation & purification; D004912:Erythrocytes; D005976:Glutaral / Q000494:pharmacology; D006386:Hemagglutination Tests / Q000379:methods*; D006358:Hot Temperature; D047348:Hydrolyzable Tannins / Q000494:pharmacology; D010325:Pasteurella / Q000276:immunology*; D011309:Preservation, Biological; D012756:Sheep / Q000097:blood; D013861:Thiocyanates / Q000494:pharmacology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D047348:Hydrolyzable Tannins; D013861:Thiocyanates; D005976:Glutaral; C009941:potassium thiocyanate