Monensin inhibits synthesis of proteoglycan, but not of hyaluronate, in chondrocytes.
Monensin (10nm-1mum) inhibited the incorporation of [(35)S]sulphate and [(3)H]glucosamine into proteoglycans by rat chondrosarcoma cells, but the incorporation of [(3)H]glucosamine into hyaluronate was unaffected. The results suggest that hyaluronate synthesis occurs in a cell compartment separate from chondroitin sulphate synthesis.
D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000096:biosynthesis; D002813:Chondrosarcoma / Q000378:metabolism; D005663:Furans / Q000494:pharmacology*; D005944:Glucosamine / Q000378:metabolism; D006027:Glycosides / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D008985:Monensin / Q000494:pharmacology*; D011509:Proteoglycans / Q000096:biosynthesis*; D051381:Rats; D013431:Sulfates / Q000378:metabolism
D005663:Furans; D006027:Glycosides; D011509:Proteoglycans; D013431:Sulfates; C008646:4-nitrophenyl beta-D-xyloside; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D008985:Monensin; D005944:Glucosamine