Tamm-Horsfall protein and erythrocyte ghosts immunologically cross-react.
Antisera were raised in the rabbit against human Tamm-Horsfall protein (THP) and erythrocyte ghosts. They were purified and exhausted with serumalbumin. Using a radioimmunoassay, antisera against THP allowed to detect in erythrocyte ghosts 41 ng of a reaction material per mg membrane protein. Ouchterlony double diffusion technique showed a similar corss-reaction and, conversely, a cross-reaction between THP and antisera to erythrocyte ghosts. These data support the view that red cell membrane contains a compound related to THP.
D000818:Animals; D003429:Cross Reactions; D004910:Erythrocyte Membrane / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D005779:Immunodiffusion; D009088:Mucoproteins / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D058949:Uromodulin
D009088:Mucoproteins; C548612:UMOD protein, human; D058949:Uromodulin