Comparison of direct harvest and cultures for karyotyping EDTA anticoagulated marrow.
Studies were undertaken to determine whether EDTA was a satisfactory anticoagulant for tissue specimens for cytogenetic analysis and to investigate a modification of a currently used culture technique for obtaining metaphases. The latter involved to prolonged exposure to very low-dose colcemid and was successful in qualitative or quantitative enhancement, or both, of the temperature yield over that obtained from direct harvest in 53% of the patients studied. EDTA is a suitable anticoagulant for cytogenetic studies of specimens from either direct harvest or short-term culture if the specimen is either processed within 24 hr after collection or diluted 1:1 with Eagles minimal essential media, supplemented with fetal bovine serum and refrigerated until processed. Success has been obtained with specimens stored up to 144 hr.
D001853:Bone Marrow / Q000187:drug effects / Q000648:ultrastructure*; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D003703:Demecolcine / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D004492:Edetic Acid / Q000494:pharmacology*; D006801:Humans; D007621:Karyotyping; D007938:Leukemia / Q000473:pathology; D008677:Metaphase / Q000187:drug effects; D008940:Mitotic Index; D013997:Time Factors
D003470:Culture Media; D004492:Edetic Acid; D003703:Demecolcine