Preparation of specific Escherichia coli K88 antisera by means of purified K88ab and K88ad antigens.
Escherichia coli K88ab and K88ad antigens were isolated from K88 antigen-producing E. coli K12 transconjugants and purified by means of repeated column chromatography. Injection of about 1 to 2 mg of the purified antigens into rabbits yielded specific K88ab and K88ad antisera. Attempts to obtain specific K88ac antiserum by means of this procedure failed.
D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000942:Antigens, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology*; D005779:Immunodiffusion; D010957:Plasmids; D011817:Rabbits / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial