Serological, functional, and immunochemical characterization of a monoclonal antibody (MoAb Q2/70) to human Ia-like antigens.
Serological and immunochemical studies showed that monoclonal antibody Q2/70 (MoAb Q2/70), produced by the hybridoma technique, is specific for human Ia-like antigens. This antibody recognizes an antigenic determinant which is different from those defining the serologic polymorphism of Ia-like antigens, and is expressed on subsets of human Ia-like molecules and on lymphoid cells from other species. MoAb Q2/70 inhibits unidirectional MLRs* between allogenic human lymphocytes, but not between murine and human lymphocytes. In ADCC* assays. MoAb Q2/70 mediates lysis of cultured human B lymphoid cells RPMI 4098, effected by murine splenocytes. The antibody is suitable to isolate immunologically functional B lymphocytes from human peripheral blood.
D000818:Animals; D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D002417:Cattle; D002469:Cell Separation; D003165:Complement System Proteins; D003602:Cytotoxicity, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats; D006168:Guinea Pigs; D000882:Haplorhini; D000949:Histocompatibility Antigens Class II; D006801:Humans; D046911:Macromolecular Substances; D051379:Mice; D011691:Puromycin / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D012397:Rosette Formation; D012756:Sheep; D013205:Staphylococcal Protein A / Q000378:metabolism; D014415:Tunicamycin / Q000494:pharmacology
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D046911:Macromolecular Substances; D013205:Staphylococcal Protein A; D014415:Tunicamycin; D011691:Puromycin; D003165:Complement System Proteins