Flow-cytometric analysis of human basophil degranulation. II. Degranulation induced by anti-IgE, anti-IgG4 and the calcium ionophore A23187.
Quantification of human basophil degranulation induced by anti-IgE, anti-IgG4, and by ionophore was performed using a flow-cytometric system. It was shown that these antibodies and ionophore can degranulate basophils in a dose-dependent manner, and that there is a wide variation in the response of basophils obtained from different individuals to these stimuli. A significant correlation was observed between the degree of degranulation induced by anti-IgE and anti-IgG4, while this was not the case for anti-IgE and ionophore. It was also shown that IgG4 myeloma protein can passively sensitive basophils. In general, degranulating efficacy was in the order of ionophore greater than anti-IgE greater than anti-IgG4, both in allergic and non-allergic individuals.
D000900:Anti-Bacterial Agents / Q000276:immunology*; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology*; D001491:Basophils / Q000166:cytology*; D000001:Calcimycin / Q000276:immunology*; D003584:Cytological Techniques; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*
D000900:Anti-Bacterial Agents; D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D007073:Immunoglobulin E; D000001:Calcimycin