Construction of a functional human suppressor tRNA gene: an approach to gene therapy for beta-thalassaemia.
A human tRNALys gene was converted to an amber suppressor by site-specific mutagenesis of the anticodon. The mutated tRNALys gene directed synthesis of a tRNA that suppressed the UAG amber nonsense mutation in beta O thalassemia mRNA. Such genes may be used to detect other nonsense mutations in mammalian cells and may provide an approach to gene therapy for beta O thalassaemia due to nonsense mutations.
D000926:Anticodon; D005796:Genes; D006801:Humans; D008239:Lysine; D009154:Mutation; D010443:Peptide Chain Termination, Translational; D012343:RNA, Transfer / Q000235:genetics*; D013489:Suppression, Genetic; D013789:Thalassemia / Q000235:genetics / Q000628:therapy*; D014158:Transcription, Genetic
D000926:Anticodon; D012343:RNA, Transfer; D008239:Lysine