The nucleotide sequence of tRNA4Val of Drosophila melanogaster. Chloroacetaldehyde modification as an aid to RNA sequencing.
The nucleotide sequence of tRNA4Val from Drosophila melanogaster was determined to be pGUUUmCCGUm1GGUG psi AGCGGDU(acp3U)AUCACA psi CUGCCmUIACAm5CGCAGAAGm7GCCCCCGGT psi CGm1AUCCCGGGCGGAAACACCA. It is probable that residue C 49 is modified to m5C. The use of tRNA modified with chloroacetaldehyde to overcome secondary structure problems in sequencing is described.
D000079:Acetaldehyde / Q000031:analogs & derivatives*; D000818:Animals; D001483:Base Sequence; D004331:Drosophila melanogaster / Q000235:genetics*; D009690:Nucleic Acid Conformation; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics*
D012346:RNA, Transfer, Amino Acyl; C004656:chloroacetaldehyde; D000079:Acetaldehyde