Primary in vitro antibody responses by purified murine B lymphocytes in serum-free defined medium.
A serum-free medium formulation that allows a primary in vitro antibody response to the nonmitogenic thymic independent antigen TNP-AECM-Ficoll is described. Purified murine B lymphocytes and adherent accessory cells are required for optimal specific responses as well as for optimal B cell survival. The medium is based on Iscove's modification of Dulbecco's modified Eagle's medium and Ham's F-12 nutrient mixture. The serum-replacing components are insulin, transferrin, and progesterone. The survival of unstimulated murine T cells in this Iscove/F12 medium is poor, but B cells survive for relatively long periods of time. This medium should prove useful in defining the molecular requirements for B lymphocyte activation by antigen.
D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D001769:Blood; D002003:Brucella abortus / Q000276:immunology; D003470:Culture Media; D005260:Female; D005362:Ficoll / Q000276:immunology; D006462:Hemolytic Plaque Technique; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D013154:Spleen / Q000166:cytology; D014301:Trinitrobenzenes / Q000276:immunology
D003470:Culture Media; D014301:Trinitrobenzenes; D005362:Ficoll