A comparison between vitamin K-dependent carboxylase from normal and warfarin-treated cows.
Detergent-solubilized microsomal preparations that catalyse the vitamin K-dependent gamma-carboxylation of glutamic acid residues in peptide and protein substrates, have been obtained from the livers of normal and warfarin-treated cows. The preparations from warfarin-treated animals contained more endogenous substrate than those from normal cows, but otherwise the two preparations were indistinguishable. The enzymes vitamin K reductase and gamma-glutamyl carboxylase, may function independently of each other in this system. They are, nevertheless, intimately linked in some way, so that the reduced vitamin K that is produced by the former enzyme can be used immediately by the latter.
D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D019735:Carbon-Carbon Ligases; D002417:Cattle; D007700:Kinetics; D008025:Ligases / Q000378:metabolism*; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D010084:Oxidation-Reduction; D011808:Quinone Reductases / Q000378:metabolism; D014812:Vitamin K / Q000494:pharmacology*; D010837:Vitamin K 1 / Q000031:analogs & derivatives / Q000494:pharmacology; D014859:Warfarin / Q000494:pharmacology*
D014812:Vitamin K; D002245:Carbon Dioxide; C028843:vitamin K1 hydroquinone; D014859:Warfarin; D010837:Vitamin K 1; D011808:Quinone Reductases; D008025:Ligases; D019735:Carbon-Carbon Ligases; C020660:glutamyl carboxylase