The repair-deficient mei-9 alpha Drosophila female potentiates chromosome loss induced in the parenteral genome by diethylnitrosamine.
Following matings of DEN-treated Xc2/BSYy+ males with repair-deficient mei-9 alpha females and ordinary females, significant increases in complete and partial sex chromosome loss as well as dramatic shifts in sex ratio were found with mei-9 alpha but not ordinary females. Accordingly, the mei-9 alpha female enhances the detection of chromosome lesions leading to chromosome loss induced in the male genome by DEN. To date, the 4 compounds tested in this way (DMN, DEN, MMS and procarbazine) exhibit strong potentiation of chromosome loss with mei-9 alpha females suggesting the possibility that a protocol involving treatment (or not) or Xc2/BSYy+ males mated with mei-9 alpha females may hold promise as an alternative to traditional tests for chromosome loss using repair-proficient females. Comparison with published translocation data on the 4 compounds indicated above suggests an overall greater sensitivity of the described mei-9 alpha chromosome-loss test compared with the traditional translocation test in the detection of chemically induced chromosome lesions.
D000818:Animals; D002872:Chromosome Deletion; D002875:Chromosomes / Q000187:drug effects; D003433:Crosses, Genetic; D004260:DNA Repair; D004052:Diethylnitrosamine / Q000494:pharmacology; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D008297:Male; D010641:Phenotype
D004052:Diethylnitrosamine