Administration of exogenous queuine is essential for the biosynthesis of the queuosine-containing transfer RNAs in the mouse.
1. Normal mouse liver contains predominantly tRNA that contains queuosine in the first position of the anticodon ((Q+)tRNA). 2. Germ-free mice fed a chemically defined diet devoid of queuine for 1 year have no queuine in all four of the tRNAs that respond to the NAUC codons. 3. The synthesis of (Q+)tRNAs can be induced by injecting queuine, feeding free queuine, or by feeding (Q+)tRNA. 4. When mice that have no (Q+)tRNA are titrated with exogenous queuine, tRNAAsp is modified to the (Q+) state before tRNAHis.
D000818:Animals; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D006151:Guanosine / Q000031:analogs & derivatives*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D009704:Nucleoside Q / Q000378:metabolism*; D012343:RNA, Transfer / Q000096:biosynthesis* / Q000302:isolation & purification
D006151:Guanosine; D009704:Nucleoside Q; D006147:Guanine; C019549:queuine; D012343:RNA, Transfer