Acid glycohydrolase in Chinese hamster with spontaneous diabetes. VII. The lack of short-term glucose-effect in cultured kidney cells.
An epithelial cell line, designated CHK-ACE, was established from the kidney of a spontaneously diabetic Chinese hamster from the highly inbred AC line. CHK-ACE was separated into two sublines, CHK-ACE-100 and CHK-ACE-400, by successive passages in 100 and 400 mg/dl glucose respectively. Extra- and intracellular activities of N-acetyl-beta-D-glucosaminidase and beta-D-galactosidase were measured in these cultures after exposure to varying concentrations of glucose (100, 200, 300 and 400 mg/dl) for one passage and 10% heated fetal calf serum for 6.5 h before enzyme measurements were taken; no apparent dependence on medium-glucose concentration was found. In serum-free medium, the time-dependent release of both N-acetyl-beta-D-glucosaminidase and beta-D-galactosidase was sustained for up to 24 h; no significant difference in their activities was found between CHK-ACE-100 cultures grown in 100 and 400 mg/dl glucose for one passage.
D000118:Acetylglucosaminidase / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D003920:Diabetes Mellitus / Q000201:enzymology*; D005696:Galactosidases / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D006596:Hexosaminidases / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D001616:beta-Galactosidase / Q000378:metabolism*
D003470:Culture Media; D005696:Galactosidases; D006596:Hexosaminidases; D001616:beta-Galactosidase; D000118:Acetylglucosaminidase; D005947:Glucose