Amino acid transport in rat Sertoli cell enriched testes: studies on the mechanism of action of follicle stimulating hormone.
1. The in vivo administration of ovine Follicle Stimulating Hormone (FSH) (15 mu g/rat, 1 h before measurements) enhanced the transport of Alpha [3-14C]-aminoisobutyric acid ([14C]-AIB) in Sertoli cell enriched testes of 10, 15 and 20-day-old rats. The effect was not observed in 5 or 25-day-old rats. 2. Increasing the intracellular concentration of cyclic AMP by means s cholera toxin, exogenous cyclic AMP or 1-methyl-3-isobutylxanthine did not change either the control or the FSH-stimulated uptake of [14C]-AIB. 3. The inhibition of glycoprotein synthesis by tunicamycin decreased the uptake of [14C]-AIB in controls and significantly reduced (24%) the effect of FSH on [14C]-AIB transport in Sertoli cell enriched testes.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000621:Aminoisobutyric Acids / Q000378:metabolism*; D000818:Animals; D002772:Cholera Toxin / Q000494:pharmacology*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D005944:Glucosamine / Q000031:analogs & derivatives*; D006023:Glycoproteins / Q000096:biosynthesis; D008297:Male; D051381:Rats; D012708:Sertoli Cells / Q000378:metabolism*; D013737:Testis / Q000378:metabolism; D014415:Tunicamycin / Q000494:pharmacology*
D000621:Aminoisobutyric Acids; D006023:Glycoproteins; D014415:Tunicamycin; D005640:Follicle Stimulating Hormone; D002772:Cholera Toxin; D000262:Adenylyl Cyclases; D005944:Glucosamine