Effects of trace amounts of Ca2+ and Mg2+ on the polymerization of actin.
At low ionic strength, removal of trace amounts of Ca2+ enhanced the polymerization of actin, and that of Mg2+ inhibited it. The actions of the divalent metal ions did not influence the elongation process of preformed nuclei. Thus it appears that the metal ions affect the nucleation step of actin polymerization.
D000199:Actins; D000818:Animals; D001718:Birefringence; D002118:Calcium; D002614:Chelating Agents; D055598:Chemical Phenomena; D002621:Chemistry; D004492:Edetic Acid / Q000031:analogs & derivatives; D004533:Egtazic Acid; D008274:Magnesium; D009994:Osmolar Concentration; D011817:Rabbits
D000199:Actins; D002614:Chelating Agents; C005390:CDTA; D004533:Egtazic Acid; D004492:Edetic Acid; D008274:Magnesium; D002118:Calcium