Detection of human and murine trophoblast-specific antigens and an assessment of their species specificity.
The distribution and species specificity of human and mouse trophoblast-specific surface antigens detected by heterologous anti-mouse ectoplacental cone (anti-EPC) trophoblast and anti-human first-trimester trophoblast plasma membrane (anti-TrPM) antisera were assessed using several immunochemical and immunolabelling assays. The findings with immunofluorescence assays using anti-EPC antiserum on monolayer cultures indicate that the expression of mouse trophoblast-specific antigens is restricted to the major trophoblast components of the mouse placenta, as well as pre- and early post-implantation trophoblast, and does not occur on fetal, amnion or tumour tissues. The anti-EPC antiserum does not cross-react with human chorionic villous trophoblast. The human trophoblast-specific antigens also displayed species specificity. The anti-TrPM antiserum showed no evidence of cross-reactivity with rhesus monkey, rabbit, guinea pig or mouse trophoblast by immunodiffusion, crossed immunoelectrophoresis, immunofluorescence or immunoperoxidase labelling.
D000818:Animals; D000941:Antigens / Q000032:analysis / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006801:Humans; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D007124:Immunoenzyme Techniques; D008253:Macaca mulatta; D051379:Mice; D011247:Pregnancy; D011817:Rabbits; D013045:Species Specificity; D014327:Trophoblasts / Q000276:immunology*
D000941:Antigens