Pyridine nucleotide involvement in rat hepatic drug metabolism: pyridine nucleotide kinetics of aniline parahydroxylation.
Apparent kinetic constant (NADPH) have been calculated for NADPH-cytochrome P450 reductase and parahydroxylase in the presence of aniline with hepatic microsomes from both phenobarbital pre-treated and untreated rats. The addition of NADH gave similar stimulation of both aniline parahydroxylation and NADPH-cytochrome P450 reductase activity in the presence of aniline. It is proposed that the increase in aniline metabolism with NADH is due to an increase in the NADPH-cytochrome P450 reductase activity which is rate limiting in microsomes from both phenobarbital and untreated rats.
D000814:Aniline Compounds / Q000378:metabolism*; D000815:Aniline Hydroxylase / Q000032:analysis; D000818:Animals; D006900:Hydroxylation; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D009243:NAD / Q000494:pharmacology*; D009249:NADP / Q000494:pharmacology*; D009251:NADPH-Ferrihemoprotein Reductase / Q000032:analysis; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D051381:Rats
D000814:Aniline Compounds; D004364:Pharmaceutical Preparations; D009243:NAD; D009249:NADP; D000815:Aniline Hydroxylase; D009251:NADPH-Ferrihemoprotein Reductase; C023650:aniline