Retinol binding protein in rat testicular cells.
Cellular retinol-binding protein (CRBP) was identified in the cytosols of cultured Sertoli cells and peritubular cells from the testes of 20-day-old rats. CRBP was not detected in spermatids or spermatocytes obtained from the testes of 60-day-old rats. Cultured Sertoli cells and peritubular cells contained up to a 5-fold enrichment of CRBP/mg protein compared to whole testis homogenates. FSH- or FSH + testosterone-treated cultures of Sertoli cells showed a 60% increase in the specific activity of CRBP when compared to untreated cultures.
D000818:Animals; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D008297:Male; D051381:Rats; D012177:Retinol-Binding Proteins / Q000378:metabolism*; D054840:Retinol-Binding Proteins, Cellular; D012671:Seminiferous Tubules / Q000166:cytology / Q000378:metabolism*; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000494:pharmacology
D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; D013739:Testosterone; D005640:Follicle Stimulating Hormone