Further structural and antigenic studies of light-chain amyloid proteins.
The major subunit protein of amyloid fibrils (758) isolated from a patient with systemic amyloidosis and studied by N-terminal amino acid sequence analysis was found to be almost identical to the sequence of a V lambda IV Bence-Jones protein and a previously described A lambda IV amyloid protein. The two A lambda IV amyloid proteins showed strong antigenic cross-reaction, appearing as antigenic identity in double immunodiffusion tests using anti-A lambda IV antiserum raised against one or the other of the two proteins. In addition, another new A lambda V amyloid fibril protein (R.S.) showed strong amino acid sequence homology and  antigenic identity in double immunodiffusions with the prototype of the A lambda V subgroup (the AR protein). Finally, 20 primary or myeloma-associated amyloid proteins were characterized using antisera against the AA and several Ig light-chain-derived amyloid proteins.
D000595:Amino Acid Sequence; D000682:Amyloid / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D000818:Animals; D000941:Antigens; D001536:Bence Jones Protein; D055598:Chemical Phenomena; D002621:Chemistry; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000145:classification*; D008970:Molecular Weight; D009101:Multiple Myeloma / Q000276:immunology; D011817:Rabbits; D000685:Serum Amyloid A Protein / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D014556:Urine / Q000276:immunology; D008258:Waldenstrom Macroglobulinemia / Q000276:immunology
D000682:Amyloid; D000941:Antigens; D007147:Immunoglobulin Light Chains; D000685:Serum Amyloid A Protein; C033078:amyloid fibril protein RS; C033077:amyloid protein 758; D001536:Bence Jones Protein