Post-transcriptional control of protein synthesis in Balb/c-3T3 cells by platelet-derived growth factor and platelet-poor plasma.
Platelet-derived growth factor (PDGF) and platelet-poor plasma, which lacks PDGF, both induce a rapid increase in the rate of total protein synthesis within quiescent, density-arrested Balb/c-3T3 cells. This stimulation of protein synthesis is associated with an increased aggregation of ribosomes into polyribosomes. Nuclear functions are not required for this response, as demonstrated by the observation that this stimulation of protein synthesis occurs in cells pretreated with actinomycin D and in enucleated cells (cytoplasts). The response to PDGF persists even after PDGF has been removed from the culture medium, but in contrast, when plasma is removed from the medium, polysomes disaggregate and protein synthesis declines. PDGF and plasma do not function synergistically to increase protein synthesis, whereas they do to induce optimum DNA synthesis. Thus stimulation of the translational apparatus may be necessary for the mitogenic response of Balb/c-3T3 cells to growth factors, but it is not by itself sufficient.
D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D006133:Growth Substances / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000494:pharmacology*; D010949:Plasma; D010982:Platelet-Derived Growth Factor; D011132:Polyribosomes / Q000378:metabolism; D011247:Pregnancy; D014176:Protein Biosynthesis / Q000187:drug effects*; D012260:Ribonucleases / Q000378:metabolism
D006133:Growth Substances; D010455:Peptides; D010982:Platelet-Derived Growth Factor; D003609:Dactinomycin; D003513:Cycloheximide; D004492:Edetic Acid; D012260:Ribonucleases