A study of the effects of glutaraldehyde and formaldehyde on the mechanical behaviour of bovine pericardium.
The inherent variability in the mechanical behavior of bovine pericardium causes difficulties in establishing the effects of treatment procedures used in the manufacture of bioprosthetic cardiac valves. A new experimental technique has been developed in which the specimen acts as its own control, obviating the need to perform large numbers of experiments to obtain statistically significant results. The procedure used is described in detail as is the equipment employed. The technique was used to assess the effect of glutaraldehyde and formaldehyde on the stress/strain response of bovine pericardium. The results show that the fixing process in glutaraldehyde is virtually complete within two hours and causes significant changes in the mechanical behaviour of the tissue. The tissue becomes progressively stiffer as the treatment period is extended, which is especially pronounced at low levels of stress. Formaldehyde storage subsequent to fixing in glutaraldehyde was found to have no effect on the stress/strain response of the bovine pericardium.
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D001705:Bioprosthesis; D002417:Cattle; D005557:Formaldehyde / Q000494:pharmacology*; D005976:Glutaral / Q000494:pharmacology*; D006350:Heart Valve Prosthesis; D010496:Pericardium / Q000187:drug effects* / Q000502:physiology; D013718:Tensile Strength / Q000187:drug effects*; D014021:Tissue Preservation / Q000379:methods*
D000447:Aldehydes; D005557:Formaldehyde; D005976:Glutaral