title: Evolution in Pseudomonas fluorescens.
abstract: The relationships among 93 strains of Pseudomonas fluorescens were investigated by (1) a numerical taxonomic analysis on the results of 150 phenotypic tests, (2) DNA hybridization studies using 16 reference strains, (3) quantitative microcomplement fixation studies using six reference strains with antibodies directed against the protein azurin. In general, the strains fell into distinct clusters. Assignment to these clusters on the basis of azurin immunological similarity showed 98% agreement with assignment based on DNA homology, suggesting that many genes will follow the same pattern. Of the strains that clustered on the basis of genotype (DNA, azurin) 88% also clustered on the basis of phenotype. The occasional noncongruency observed between the genotypic and phenotypic data may be due to the variable rates of phenotypic evolution. These results provide a perspective on the roles of horizontal and vertical transfer of genes in the evolution of this bacterial group.
mesh_terms: D001400:Azurin; D005075:Biological Evolution; D004269:DNA, Bacterial; D005838:Genotype; D010641:Phenotype; D011550:Pseudomonas aeruginosa / Q000145:classification; D011551:Pseudomonas fluorescens / Q000145:classification / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D001400:Azurin