title: Characteristics of Murayama virus in various cell cultures and laboratory animals.
abstract: Some biological properties of Murayama virus, a new paramyxovirus, were studied. The virus grew well in primary monkey kidney cells as well as embryonated eggs, while the virus yields in primary chick embryo and BHK-21 cells were much lower. The infected BHK-21 cells formed large syncytia and showed typical hemadsorption, but did not produce any detectable amount of hemagglutinin in the culture fluid. The virus yields were very low in Vero. LLC-MK2 and MDCK cells at first passages. The addition of trypsin to the medium enhanced virus growth in Vero and LLC-MK2 but not in MDCK cells. Cell fusion activity of the virus was observed in Molt-4 cells. Hemolytic activity was enhanced by freeze-thawing. Several species of mammals and birds were susceptible to experimental infections with the virus as evidenced by seroconversion and positive virus isolation without showing any clinical signs.
mesh_terms: D000818:Animals; D000830:Animals, Laboratory / Q000382:microbiology; D001717:Birds; D002459:Cell Fusion; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D006224:Cricetinae; D005615:Freezing; D006168:Guinea Pigs; D000882:Haplorhini; D007668:Kidney / Q000382:microbiology; D051379:Mice; D010252:Paramyxoviridae / Q000254:growth & development* / Q000472:pathogenicity; D014357:Trypsin / Q000494:pharmacology
chemical_list: D014357:Trypsin