title: Genetic dissection of clonally inherited genomes of Poeciliopsis: II. Investigation of a silent carboxylesterase allele.
abstract: According to the ratchet mechanism hypothesis, deleterious mutations should accumulate in clonal genomes of unisexual fish of the genus Poeciliopsis. This study defines one such mutant, a silent carboxylesterase allele (ES-5 degrees) which is found in the heterozygous condition in a particular population of P. monacha-occidentalis. An antiserum to purified Poeciliopsis carboxylesterase cross-reacts with the gene product of the Es-5 degrees allele upon immunoelectrophoresis. This finding of cross-reacting material associated with the Es-5 degrees allele provides a useful marker for the breeding of a carboxylesterase deficient strain.
mesh_terms: D000483:Alleles; D000818:Animals; D000936:Antigen-Antibody Complex; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D005399:Fishes / Q000235:genetics*; D006579:Heterozygote; D007106:Immune Sera; D005779:Immunodiffusion; D007527:Isoenzymes / Q000235:genetics; D009154:Mutation; D010641:Phenotype
chemical_list: D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007527:Isoenzymes; D002265:Carboxylic Ester Hydrolases