[Concentration and purification of alkaline proteinase by ultrafiltration].
The mode of ultrafiltration of the culture fluid of Bacillus subtilis, producer of alkaline proteinase, through semipermeable acetate cellulose membranes was examined. The ultrafiltration of the enzyme-containing solutions involved both concentration of the solution and enzyme purification from low molecular weight admixtures. The optimum mode of ultrafiltration of alkaline proteinase on UAM membranes "Vladipore" was developed.
D001412:Bacillus subtilis / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010447:Peptide Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D014462:Ultrafiltration
D010447:Peptide Hydrolases
Kontsentrirovanie i ochistka rastvorov shchelochnoĭ proteinazy metodom ul'trafil'tratsii.