Preparation of anti-Gm sera by immunization of rabbits with protein A-fractionated normal IgG proteins from Japanese: further study.
A method is described for the production of rabbit anti-Gm sera using normal IgG proteins and fragments thereof as the immunogens. Anti-G3m(16) and anti-G3m(21) were readily producible by immunizations with protein A-purified IgG3 proteins of G3m(13, 15, 16) and G3m(21) type, respectively. Anti-G1m(2), anti-G1m(3), and anti-G3m(5) were obtained by immunizations with type Gm(1, 2, 21) Fc fragments, type Gm(1, 3, 5, 13) IgG1-rich IgG, and type G3m(5, 13, 21) IgG3, respectively. These immune sera can distinguish the 9 Gm phenotypes in the Japanese.
D000818:Animals; D000917:Antibody Formation; D005591:Chemical Fractionation; D007114:Immunization; D007141:Immunoglobulin Fc Fragments / Q000276:immunology*; D007074:Immunoglobulin G; D007129:Immunoglobulin Gm Allotypes; D007143:Immunoglobulin Heavy Chains / Q000276:immunology*; D007142:Immunoglobulin gamma-Chains / Q000276:immunology*; D007564:Japan; D008297:Male; D009194:Myeloma Proteins / Q000276:immunology; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000494:pharmacology*
D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007129:Immunoglobulin Gm Allotypes; D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains; D009194:Myeloma Proteins; D013205:Staphylococcal Protein A; C012335:Gm(m)