Detection of deoxyribonuclease activities of Mycoplasma pneumoniae.
Alkaline deoxyribonuclease activity was detected in culture of Mycoplasma pneumoniae. This enzyme required MgCl2 to stimulate its activity and preferred double-stranded to single-stranded DNA as substrate. By using polyacrylamide gel electrophoresis or isoelectric electrofocusing, two distinctive deoxyribonuclease activities were demonstrated both in cultured broth and cell homogenate.
D003851:Deoxyribonucleases / Q000032:analysis* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D009177:Mycoplasma pneumoniae / Q000201:enzymology*
D003851:Deoxyribonucleases