Heat shock induces rapid dephosphorylation of a ribosomal protein in Drosophila.
Ribosomes isolated from Drosophila melanogaster tissue culture cells labeled in vivo with 32Pi contain a single, heavily phosphorylated, ribosomal protein. As much as 40% of this protein is phosphorylated in cells cultured at 25 degrees C. The molecular weight and other characteristics of this protein suggest possible homology with ribosomal protein S6. Following a shift-up to 37 degrees C, the protein is specifically and quantitatively dephosphorylated. The kinetics of this dephosphorylation are rapid with a half-time on the order of a few minutes. These kinetics closely parallel the heat shock-induced breakdown of the preexisting polysome population.
D000818:Animals; D002478:Cells, Cultured; D004331:Drosophila melanogaster / Q000235:genetics*; D006657:Histones / Q000378:metabolism; D006358:Hot Temperature; D007700:Kinetics; D010710:Phosphates / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism*
D006657:Histones; D010710:Phosphates; D010750:Phosphoproteins; D012269:Ribosomal Proteins