Identification of immunoglobulin heavy-chain isotypes of specific antibodies of horse 46 group B meningococcal antiserum.
Hyperimmune horse serum from a single animal (horse 46) immunized with group B (strain B-11) meningococcal vaccine provides a standardized, readily available diagnostic reagent used in primary isolation medium and for serogrouping of meningococci. Identification of the heavy-chain isotypes of specific anticapsular polysaccharide and anti-lipopolysaccharide isolated from horse 46 serum revealed a differential distribution in the occurrence of immunoglobulin classes. Meningococcal anticapsular antibodies of horse 46 serum were restricted predominately to the immunoglobulin M (IgM) class, with only trace amounts of IgGa present, whereas anti-lipopolysaccharide concomitantly produced showed a heterogeneity in its heavy-chain isotypes, consisting of IgM, IgGa, IgGb, moderate amounts of IgB, and a small amount of IgA.
D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D002850:Chromatography, Gel; D006736:Horses / Q000276:immunology*; D007106:Immune Sera / Q000032:analysis*; D007114:Immunization; D005779:Immunodiffusion; D007143:Immunoglobulin Heavy Chains / Q000302:isolation & purification*; D009345:Neisseria meningitidis / Q000276:immunology*
D000907:Antibodies, Bacterial; D007106:Immune Sera; D007143:Immunoglobulin Heavy Chains