Radioimmunoassay of PCBs in milk and blood.
A radioimmunoassay was developed capable of determining Aroclor 1260 in milk at levels of from 20 to 80 ppb and in blood from 2 to 16 ppb. The values obtained by radioimmunoassay correlate well with those determined by gas-liquid chromatography (r2 = 0.96 for milk and 0.99 for blood) but were lower. Antiserum was produced in rabbits and was specific for 2,2',4,4',5,5'-hexachlorobiphenyl. It cross-reacted with congeners and isomers in Aroclor 1254 and 1260 to the extent that a 20% decrease in binding was observed with 0.1 ng of either mixture. The method requires preliminary cleanup of the extract on alumina and utilizes 25% dimethyl sulfoxide in the assay medium to promote solubilization of the substrates.
D000818:Animals; D001140:Aroclors / Q000097:blood; D002417:Cattle; D002849:Chromatography, Gas; D003429:Cross Reactions; D006801:Humans; D008892:Milk / Q000032:analysis*; D011078:Polychlorinated Biphenyls / Q000097:blood*; D011863:Radioimmunoassay; D012016:Reference Values
D001140:Aroclors; D011078:Polychlorinated Biphenyls