title: Long-term in vitro culture of murine mast cells. III. Discrimination of mast cells growth factor and granulocyte-CSF.
abstract: Long-term in vitro growth of murine mast cells was dependent on the presence of a mast cell growth factor (MCGF) present in media conditioned by mitogen-activated splenic leukocytes or by various murine leukemic cell lines. MCGF shared a number of properties with granulocyte colony-stimulating factor (G-CSF). Both factors were present in media conditioned by the myelomonocytic leukemic WEHI-3 and the T cell lymphoma, LBRM-33 cell lines. They were relatively sensitive to trypsin treatment, and were resistant to boiling temperature. NZB mice that failed to respond to WEHI-3-derived G-CSF also failed to respond to MCGF. MCGF differed from G-CSF, however, in sensitivity to neuraminidase and lactoferrin, an inhibitor of macrophage CSF production, suppressed G-CSF production by WEHI-3 cells without affecting MCGF production. Furthermore, peritoneal cells produced G-CSF but not MCGF when stimulated with lipopolysaccharide. In vitro production of MCGF by normal spleen cells required the presence of T lymphocytes and is relatively macrophage-independent. The role of T cells in the maturation and growth of mast cells and the physiologic function of MCGF are discussed.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000032:analysis*; D006098:Granulocytes / Q000502:physiology*; D006133:Growth Substances / Q000032:analysis*; D006358:Hot Temperature; D007376:Interleukin-2 / Q000032:analysis*; D007377:Interleukin-3; D008070:Lipopolysaccharides / Q000494:pharmacology; D008222:Lymphokines / Q000032:analysis*; D008407:Mast Cells / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains / Q000276:immunology; D009439:Neuraminidase / Q000378:metabolism; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors; D014357:Trypsin / Q000378:metabolism
chemical_list: D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007376:Interleukin-2; D007377:Interleukin-3; D008070:Lipopolysaccharides; D008222:Lymphokines; D009439:Neuraminidase; D014357:Trypsin