A xenogeneic monoclonal antibody recognizing specificities controlled by HLA-A and B alleles.
In the present paper one reagent among the many prepared has been carefully studied. It is a xenogeneic monoclonal antibody, F10.13/13, obtained by immunizing mice with human peripheral blood lymphocytes. The splenocytes of the immunized mice were fused with a murine were fused with a murine myeloma and the supernatants of the resulting Ig-secreting hybridomas were tested against appropriate targets. --F10.13/13 behaves in a very peculiar manner from the serological point of view and we think that it reacts with maximal affinity with an epitope expressed most strongly on HLA glycoproteins controlled by genes B8, B7, and Aw19.
D000483:Alleles; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001665:Binding Sites; D002999:Clone Cells / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006680:HLA Antigens / Q000235:genetics*; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G; D051379:Mice; D008807:Mice, Inbred BALB C; D013205:Staphylococcal Protein A / Q000378:metabolism
D000906:Antibodies; D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A