Application of monoclonal anti-HY antibody for human H-Y typing.
We have successfully produced monoclonal anti- H-Y antibody by fusing NS-1 myeloma cells with splenocytes from C57BL/6 females immunized with syngeneic male splenocytes. We have proved that the antibody is male specific and that it cross reacts with human H-Y. We have further tested 80 normal individuals for H-Y antigen and obtained significant differences between males and females. Therefore, the monoclonal anti- H-Y antibody is useful for clinical typing of human H-Y antigen.
D000818:Animals; D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D005260:Female; D005347:Fibroblasts / Q000276:immunology; D006059:Gonadal Dysgenesis / Q000235:genetics; D006182:H-Y Antigen / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology; D007621:Karyotyping; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010954:Plasmacytoma; D011898:Ranidae; D012397:Rosette Formation; D013154:Spleen / Q000276:immunology; D014424:Turner Syndrome / Q000235:genetics
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D006182:H-Y Antigen