Detection of type D retrovirus in a human amnion cell line (FL).
This paper describes the detection of viral particles in a human amnion cell line (FL). These particles belong to the group of type D retroviruses, because of their characteristic morphology, their major antigenic determinants, and the presence of particle-associated Mg++-preferring RNA-dependent DNA polymerase. In addition to budding and mature type D virus particles, intracytoplasmic type A structures have also been found. Immunological analysis provided evidence of cross-reactivity between the particles described and the Mason-Pfizer monkey virus as well as type D retroviruses from other human cell lines (PMF, HEp-2). Particles isolated from FL cell medium were shown to infect type D virus-susceptible human TU 197 cells.
D000650:Amnion / Q000166:cytology / Q000382:microbiology*; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D005779:Immunodiffusion; D008854:Microscopy, Electron; D012190:Retroviridae / Q000302:isolation & purification*
D000956:Antigens, Viral