Performance of a commercial microdilution minimal inhibitory concentration procedure for aminoglycoside susceptibility testing of Pseudomonas aeruginosa.
The accuracy of quantitative aminoglycoside minimal inhibitory concentration (MIC) determinations was evaluated with the Micro-Media Systems microdilution MIC panel and Pseudomonas aeruginosa. Without Mg++ and Ca++ supplementation, very major interpretive discrepancies occurred. A simple, in-expensive cation supplementation procedure was evaluated to correct the discrepancies. The necessity of cation supplementation for susceptibility testing of P. aeruginosa when employing MIC procedures is stressed.
D000617:Aminoglycosides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002118:Calcium / Q000008:administration & dosage; D003470:Culture Media; D005839:Gentamicins / Q000008:administration & dosage; D008274:Magnesium / Q000008:administration & dosage; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development; D014031:Tobramycin / Q000008:administration & dosage
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D003470:Culture Media; D005839:Gentamicins; D008274:Magnesium; D002118:Calcium; D014031:Tobramycin