[Potentiometric study of the progress of the glycogen synthetase reaction].
A procedure for continuous recording of the glycogen synthetase 1 activity, based on the potentiometry, is described. The stoichiometric ratio of H+/UDP, estimated at pH 7.8 and 30 degrees, was equal to 0.89 +/- 0.01. The specific activity of the enzyme did not depend on the buffer capacity in the incubation mixture containing 0.8-10 mM Tris-HCl, pH 7.8. The course of reaction had a linear type at the enzyme concentration of 4-20 mcg/ml in presence of 10 mM MgCl2. The rate of glycogen synthetase reaction was decreased in the course of experiment if the activator was absent. MgCl2 was more suitable activator than Na2SO4, universally accepted for this form of the enzyme.
D000818:Animals; D002384:Catalysis; D002712:Chlorides / Q000494:pharmacology; D004789:Enzyme Activation; D006006:Glycogen Synthase / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D009132:Muscles / Q000201:enzymology*; D011199:Potentiometry; D011817:Rabbits; D012964:Sodium / Q000494:pharmacology; D013431:Sulfates / Q000494:pharmacology
D002712:Chlorides; D013431:Sulfates; D012964:Sodium; D006006:Glycogen Synthase; D008274:Magnesium
Izuchenie khoda glikogensintetaznoĭ reaktsii vo vremeni potentsiometricheskim metodom.