The role of serine hydroxymethyltransferase in cell proliferation: DNA synthesis from serine following mitogenic stimulation of lymphocytes.
It is shown that the time-course of incorporation of radioactivity from [3-14C]serine into nucleic acids parallels DNA synthesis following mitogenic stimulation of human peripheral blood lymphocytes by phytohaemagglutinin (PHA). The activity of serine hydroxymethyltransferase was elevated about four-fold in PHA-stimulated lymphocytes compared to that in unstimulated control cells. It is suggested that lymphocytes, in common with other proliferating cell systems, may synthesize serine de novo for utilization in pathways of nucleotide biosynthesis following mitogenic stimulation.
D002455:Cell Division; D004247:DNA / Q000096:biosynthesis*; D012696:Glycine Hydroxymethyltransferase / Q000378:metabolism*; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology; D012694:Serine / Q000378:metabolism*; D014166:Transferases / Q000378:metabolism*
D010835:Phytohemagglutinins; D012694:Serine; D004247:DNA; D014166:Transferases; D012696:Glycine Hydroxymethyltransferase