title: Use of the intracerebral injection technique to elucidate mechanisms of apomorphine climbing and its antagonism in the mouse.
abstract: Climbing behavior induced by peripherally administered apomorphine in the mouse was reduced by 0.25-10 microgram bilateral intra-accumbens fluphenazine, (+/-) and (-) sulpiride and by serotonin, but not by (+)sulpiride, dl-propranolol, phentolamine, atropine or methysergide. A specific antagonism of climbing could not be shown when fluphenazine was injected into the striatum, hypothalamus, thalamus, reticular formation, frontal cortex or cerebellum, but was apparent when a large dose of fluphenazine was placed below (but not above) the accumbens nucleus. 6-Hydroxydopamine denervation of the nucleus accumbens did not alter the climbing antagonism afforded by fluphenazine, although sulpiride was three-fold more effective following denervation. The data indicates an accumbens involvement in the climbing phenomenon, that sulpiride more effectively antagonises climbing after accumbens denervation and that the presumed dopamine agonist-antagonist interaction in the accumbens, which controls climbing, may also involve serotonergic function. The studies emphasise the value of the intra-cerebral injection technique to an analysis of drug action in the mouse.
mesh_terms: D000818:Animals; D001058:Apomorphine / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D001921:Brain; D004305:Dose-Response Relationship, Drug; D005476:Fluphenazine / Q000627:therapeutic use; D006801:Humans; D006892:Hydroxydopamines / Q000494:pharmacology; D007267:Injections; D008297:Male; D051379:Mice; D013239:Stereotyped Behavior / Q000187:drug effects*; D013469:Sulpiride / Q000627:therapeutic use
chemical_list: D006892:Hydroxydopamines; D013469:Sulpiride; D001058:Apomorphine; D005476:Fluphenazine