Preparation of agglutinating antisera specific for the flagellar antigens of Pseudomonas aeruginosa.
H-specific agglutinating sera to Pseudomonas aeruginosa were prepared by immunisation with partially purified flagella. The results of agglutination and immobilisation tests with rabbit sera prepared against the flagella of six H-type strains showed that the sera had high titres and were H specific. Cross-absorption tests revealed that one strain (H-3) possessed a distinct antigen not present in any of the others. Two groups of strains (H-1, H-2 and H-5; H-4 and H-6) each possessed a distinct major antigen. Members of these two groups could be distinguished by their minor antigens.
D000371:Agglutination; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D005407:Flagella / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D011817:Rabbits; D012703:Serotyping
D000942:Antigens, Bacterial; D007106:Immune Sera