[Preliminary observations on in vitro biosynthesis of prostaglandins E in tumor cells and virus transformed cells].
Biosynthesis of prostaglandins of E series in BK virus-transformed rabbit-kidney cells (RKBK) and mouse hepatoma cells cultured in vitro with and without exogenous arachidonic acid was determined by radioimmunoassay. Elevated concentrations of prostaglandin E2 were observed in both cell types; moreover, tumor and transformed cells were capable to synthesize higher levels of prostaglandin E2 than normal cells. Mouse hepatoma cells produced more prostaglandins compared to RKBK cells; in addition they seemed less sensitive to inhibition by indomethacin. These data suggest that arachidonic acid metabolism may be another one of the intrinsic biochemical properties that differentiate tumor and virus transformed cells.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002460:Cell Line; D002472:Cell Transformation, Viral; D015232:Dinoprostone; D007668:Kidney / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D051379:Mice; D011458:Prostaglandins E / Q000096:biosynthesis*; D011817:Rabbits; D011863:Radioimmunoassay
D001095:Arachidonic Acids; D011458:Prostaglandins E; D016718:Arachidonic Acid; D015232:Dinoprostone
Osservazioni preliminari sulla biosintesi in vitro di prostaglandine di tipo E in cellule tumorali e trasformate da virus.