Production of T-cell lines with inhibitory or stimulatory activity against syngeneic tumors in vivo. A preliminary report.
We obtained Thy-I-positive cells directly from growing methylcholanthrene-induced (MCA-1510) sarcomas using fluorescence-activated cell sorting, then cultured these lymphocytes in medium containing Interleukin-2 and tested their activity in vivo against various MCA-sarcoma lines with the Winn assay. We found that cultured T cells from small MCA-1510 tumors (17 days after transplantation) significantly inhibited the growth of that particular sarcoma, but not of three other MCA-tumor lines tested, while cultured T cells from large MCA-1510 sarcomas (41 days after transplantation) significantly enhanced the growth of that tumor, but not of an unrelated tumor, MCA-1460. The former cells were primarily Lyt-1+, 2+ while the latter were primarily Lyt-1+, 2+.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D005354:Fibrosarcoma / Q000139:chemically induced / Q000276:immunology; D007375:Interleukin-1; D008297:Male; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D011506:Proteins; D012513:Sarcoma, Experimental / Q000139:chemically induced / Q000276:immunology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D007375:Interleukin-1; D011506:Proteins; D008748:Methylcholanthrene