title: [T-lymphocyte activation. Studies on the function of mediator proteins (author's transl)].
abstract: The functions of several mediator proteins involved in the T-cell blastogenesis have been investigated 1. Newly described is the Plasmatic Human IL-2 Inducing Protein (PHILIP), a glycoprotein from human serum with a molecular weight of about 85,000 D. Its presence is mandatory for the synthesis and/or secretion of Interleukin-2 (T-cell growth factor). The mediator protein can be distinguished from other known serum glycoproteins (e.g., transferrin and plasminogen) by affinity chromatography. Desialylation completely abolishes its biologic activity. 2. PMSF- and DFP-treatment of conditioned culture medium inhibit the blastogenesis in the peripheral mononuclear blood-cell fraction. However, the growth of a permanent T-cell line in the inhibitor-treated medium is not affected. This indicates the existence of a blastogenic factor with serine-protease activity.
mesh_terms: D000208:Acute Disease; D006023:Glycoproteins / Q000097:blood*; D006505:Hepatitis / Q000276:immunology; D006801:Humans; D007375:Interleukin-1; D007376:Interleukin-2 / Q000502:physiology*; D008103:Liver Cirrhosis / Q000276:immunology; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000502:physiology*; D008970:Molecular Weight; D011506:Proteins / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D006023:Glycoproteins; D007375:Interleukin-1; D007376:Interleukin-2; D008222:Lymphokines; C031501:PHILIP protein, human; D011506:Proteins
vernacular_title: T-Lymphocyten-Aktivierung. Untersuchungen zur Funktion von Mediatorproteinen.