Demonstrations of the production of specific antibodies to poly(I).poly(C) in rabbits.
The rabbit antiserum against poly(I).poly(C) purified by hydroxyapatite column chromatography contained three distinct antibodies. They were fractionated into three antibody populations by a series of precipitations (with poly(A).poly(U), poly(I), and poly(I).poly(C)) and their specificities were examined by quantitative complement fixation, double diffusion tests and radioimmunoassay. The first population was common to the double helical structure of double-stranded RNAs. The second was specific for poly(I) and the third was specific for poly(I).poly(C). These studies demonstrated that specific antibodies exclusively reactive with poly(I).poly(C) existed in the rabbit antiserum against poly(I).poly(C).
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000918:Antibody Specificity; D003168:Complement Fixation Tests; D005779:Immunodiffusion; D007163:Immunosorbent Techniques; D011069:Poly I / Q000276:immunology; D011070:Poly I-C / Q000276:immunology*; D012330:RNA, Double-Stranded / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay
D000906:Antibodies; D012330:RNA, Double-Stranded; D011069:Poly I; D011070:Poly I-C