DIG-ELISA for the serologic diagnosis of toxoplasmosis.
A new serologic method, diffusion in gel-enzyme-linked immunoassay (DIG-ELISA) is described and compared with the indirect fluorescent antibody (IFA) and indirect hemagglutination assay (IHA) for the serologic diagnosis of toxoplasmosis. The results demonstrate that DIG-ELISA is a viable alternative to IFA and IHA because of its simplicity, economy, versatility, and potential for standardization.
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D005779:Immunodiffusion / Q000379:methods*; D007124:Immunoenzyme Techniques; D051379:Mice; D012698:Serologic Tests / Q000379:methods; D014123:Toxoplasmosis / Q000175:diagnosis*