Detergent-induced proteolysis of rabbit intestinal brush border vesicles.
Proteolysis of brush border vesicle proteins is induced by detergent solubilisation. This proteolysis is selective in that some of the proteins are more susceptible than others. The rate of induced proteolysis is decreased by decreasing the temperature, has a minimum of about pH 6 and is effectively prevented by a combination of the protease inhibitors, EDTA, diisopropylfluorophosphate and iodoacetamide.
D000199:Actins / Q000378:metabolism; D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism*; D003902:Detergents / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D007421:Intestine, Small / Q000378:metabolism*; D007460:Iodoacetamide / Q000494:pharmacology; D007531:Isoflurophate / Q000494:pharmacology; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism*; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism*; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D011817:Rabbits; D013501:Surface-Active Agents / Q000494:pharmacology*
D000199:Actins; D001647:Bile Acids and Salts; D003902:Detergents; D008565:Membrane Proteins; D013501:Surface-Active Agents; D007531:Isoflurophate; D011092:Polyethylene Glycols; D017830:Octoxynol; D004492:Edetic Acid; D007460:Iodoacetamide