[Rapid microbiological method of determining the gentamicin, sisomicin and kanamycin concentrations in patients' serum].
A modification of the microbiological agar-diffusion method for rapid determination of gentamicin, sisomycin and kanamycin levels in the blood serum of patients is described. The decrease in the time for determination of the antibiotic levels in the serum specimens with the modified method was provided by the use of a higher inoculation dose of the test microbe, higher levels of the incubation temperature and an enriched nutrient medium. The assay time was decreased from 18 to 3--4 hours as compared to the routine agar-diffusion method.
D001412:Bacillus subtilis / Q000187:drug effects; D001681:Biological Assay / Q000379:methods*; D003470:Culture Media; D005839:Gentamicins / Q000097:blood*; D006801:Humans; D007612:Kanamycin / Q000097:blood*; D012853:Sisomicin / Q000097:blood*; D013171:Spores, Bacterial / Q000187:drug effects; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D005839:Gentamicins; D007612:Kanamycin; D012853:Sisomicin
Uskorennyĭ mikrobiologicheskiĭ metod opredeleniia kontsetratsiĭ gentamitsina, sizomitsina i kanamitsina v syvorotke krovi bol'nykh.