Immunological properties and immunohistochemical localization of cysteine sulfinate or aspartate aminotransferase-isoenzymes in rat CNS.
Organ and interspecies specificities of cysteine sulfinate transaminase isoenzymes were studied by double immunodiffusion. Antisera were produced in rabbits against purified mitochondrial and cytosolic enzymes of rat brain. No cross-reaction was found between the two isoenzymes. The enzymes present in crude mitochondrial fractions prepared from rat brain, heart, kidney and liver are immunologically identical. Similar results were obtained with the enzymes present in cytosolic fractions. Mouse, chicken, frog and rat crude mitochondrial and soluble fractions were tested, but only mouse isoenzymes reacted. The localizations of mitochondrial and cytosolic cysteine sulfinate transaminases were studied in the rat olfactory bulb and retina. The mitochondrial enzyme was found essentially in the plexiform, glomeruli and mitral cell layers of the olfactory bulb, and in the inner segment and external plexiform layer of the retina, whereas the cytosolic enzyme was located mainly in the granule cell areas of the olfactory bulb and in the nuclear layer of the retina. These results revealed a lack of organ specificity, an interspecies specificity, and a specific localization indicating specific roles for both isoenzymes.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000276:immunology*; D001921:Brain / Q000276:immunology*; D002645:Chickens; D003600:Cytosol / Q000276:immunology; D005779:Immunodiffusion; D007527:Isoenzymes / Q000276:immunology*; D051379:Mice; D008928:Mitochondria / Q000276:immunology; D009830:Olfactory Bulb / Q000276:immunology; D011898:Ranidae; D051381:Rats; D012160:Retina / Q000276:immunology; D013045:Species Specificity; D000637:Transaminases / Q000276:immunology*
D007527:Isoenzymes; D000637:Transaminases; C019973:cysteine sulfinate transaminase; D001219:Aspartate Aminotransferases