Ready separation of proteins from nucleoprotein complexes by reversible modification of lysine residues.
Modification of proteins with citraconic anhydride altered the electrostatic relationship between cationic epsilon-NH3+ groups of lysine residues of proteins and anionic phosphate groups of nucleic acids, thereby destabilizing the nucleoprotein complex. This procedure facilitated the separation of proteins from nucleic acids at pH4-4.2. The modifying groups were then deacylated from the proteins under acidic conditions (pH3-6) at 30 degrees C.
D002949:Citraconic Anhydrides; D005656:Fungal Proteins / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008239:Lysine; D008722:Methods; D009698:Nucleoproteins; D012441:Saccharomyces cerevisiae / Q000032:analysis
D002949:Citraconic Anhydrides; D005656:Fungal Proteins; D009698:Nucleoproteins; D008239:Lysine