Effect of arachidonic acid on the hexose monophosphate shunt and related coenzymes in human blood platelets.
The aggregation of human platelets after addition of arachidonic acid (AA) is accompanied by a 30 fold increase in the net flux through the hexose monophosphate shunt (HMPS). The levels of reduced glutathione (GSH) and NADPH as well as the NADPH/NADPH+NADP quotient show a temporary fall which is restricted to the interval between AA addition and beginning of aggregation suggesting a lag phase between the onset of enhanced coenzyme consumption and that of increased coenzyme regeneration. together with literature data the results point to a possible regulatory function of reduced coenzymes and the HMPS in the process of platelet activation.
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology*; D001792:Blood Platelets / Q000378:metabolism*; D005978:Glutathione / Q000097:blood; D006600:Hexosephosphates / Q000097:blood*; D006801:Humans; D007700:Kinetics; D009249:NADP / Q000097:blood; D010084:Oxidation-Reduction; D010974:Platelet Aggregation / Q000187:drug effects*
D001095:Arachidonic Acids; D006600:Hexosephosphates; D016718:Arachidonic Acid; D009249:NADP; D005978:Glutathione