title: The mucociliary activity of the respiratory tract. I. Inhibitory effects of products of Pseudomonas aeruginosa on rabbit trachea in vitro.
abstract: Bacteriological filtrates of three strains of Pseudomonas aeruginosa were compared with respect to inhibitory effect on ciliary movements and a quantitative difference was established between them. The cilia inhibitory effect was strictly concentration-dependent and was resistant to heating. The ciliotoxic effect disappeared from filtrates after chloroform extraction. The chloroform-extracted sediment ws dissolved in physiological saline and the solution revealed an inhibitory effect on cilia. Involvement of endotoxin is not probable, since E. coli endotoxin in a high concentration was not toxic. Partially purified Pseudomonas aeruginosa phenazine pigment as well as haemolysin inhibited ciliary activity and the effect was standardized in the present experimental system.
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D002923:Cilia / Q000502:physiology*; D006460:Hemolysin Proteins / Q000494:pharmacology; D009093:Mucus / Q000502:physiology*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*; D011710:Pyocyanine / Q000494:pharmacology; D011817:Rabbits; D014132:Trachea / Q000502:physiology*
chemical_list: D006460:Hemolysin Proteins; D011710:Pyocyanine