Ultrastructural changes of epithelium-connective tissue junction in experimental lingual tumors.
In this study, plaque-like lesions, epithelial hyperplasias, and papillary invasive carcinomas were produced in the tongues of hamsters by the application of DMBA combined with trauma for 16 weeks. The junction between the neoplastic epithelial cells and the connective tissue was studied ultrastructurally. In plaque-like lesions, the basal lamina was partially decreased in both thickness and density or partially discontinuous. In epithelial hyperplasias, cytoplasmic projections were observed in the portion of the basal cell near the intercellular space, and the basal lamina was absent around these cytoplasmic projections. In papillary invasive carcinomas, however, numerous cytoplasmic projections extended from various portions of the basal surface of the basal cells into the underlying connective tissue. The basal lamina was also absent around these projections. In epithelial hyperplasias and papillary invasive carcinomas, some of the cytoplasmic projections appeared almost empty, some contained a small number of tonofilaments and ribosomes, and others were filled with a large number of ribosomes but did not contain the tonofilaments. The collagen fibrils were markedly reduced in number in the area around cytoplasmic projections. The cytoplasm of some fibroblasts in that area contained many intracellular collagen fibrils.
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D003238:Connective Tissue / Q000648:ultrastructure; D006224:Cricetinae; D003593:Cytoplasm / Q000648:ultrastructure; D004848:Epithelium / Q000648:ultrastructure; D007365:Intercellular Junctions / Q000648:ultrastructure*; D008647:Mesocricetus; D009374:Neoplasms, Experimental / Q000648:ultrastructure; D009940:Organoids / Q000648:ultrastructure; D014062:Tongue Neoplasms / Q000648:ultrastructure*
D015127:9,10-Dimethyl-1,2-benzanthracene