Detection of Neisseria gonorrhoeae by ultraviolet illumination of NADH generated by an immobilized antibody-gonococcal enzyme complex.
Frozen, cervical swabs were placed in a lysis buffer containing an immobilized antibody to the gonococcal enzyme, 1,2-propanediol oxidoreductase. The immobilized antibody--enzyme complex that formed was active after the addition of substrate (1,2-propanediol and NAD) and this activity could be detected by visual inspection of NADH fluorescence under ultraviolet illumination.
D000429:Alcohol Oxidoreductases / Q000302:isolation & purification; D002584:Cervix Uteri / Q000382:microbiology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006069:Gonorrhea / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D009243:NAD / Q000276:immunology*; D009344:Neisseria gonorrhoeae / Q000201:enzymology / Q000302:isolation & purification*
D009243:NAD; D000429:Alcohol Oxidoreductases; C021494:lactaldehyde reductase