Studies on subunit components of immunoglobulin M from a bony fish, the chum salmon (Oncorhynchus keta).
Immunoglobulin M (IgM) was isolated from serum of the chum salmon (Oncorhynchus keta) by means of ion-exchange chromatography followed by gel filtration. The purified chum salmon IgM had a mol. wt of 730,000 and a tetrameric structure. However, a fraction of tetrameric IgM was considered to be non-covalently associated molecules. The amino acid composition was determined for the chum salmon micro-and L-chains and was found to be similar to that reported for other teleost fish micro- and L-chains. No J-chain- like component could be identified in the salmon IgM by either alkaline urea polyacrylamide gel electrophoresis or by the immunological cross-reaction with antisera to the human and chicken J-chain. The immunoglobulin cross-reactivity between the chum salmon micro-chain and that of 22 other fishes was tested, and only micro-chains from the family Salmonidae revealed cross-reactivity.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D003429:Cross Reactions; D007144:Immunoglobulin J-Chains / Q000032:analysis; D007147:Immunoglobulin Light Chains / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007148:Immunoglobulin mu-Chains / Q000032:analysis; D046911:Macromolecular Substances; D008970:Molecular Weight; D012474:Salmon / Q000276:immunology*; D013045:Species Specificity
D000596:Amino Acids; D000888:Antibodies, Anti-Idiotypic; D007144:Immunoglobulin J-Chains; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains; D046911:Macromolecular Substances