Cell-free expression of the beta-galactosidase gene: a model system to study the effects of nucleotide analogs on transcription-translation.
A cell-free system for the expression of the beta-galactosidase gene was employed to study the effects of the UTP and CTP analogs: s2UTP, s2CTP, f5UTP and rTTP on transcription-translation. From the analogs investigated, only rTTP turned out to be able to substitute UTP in the cell-free synthesis of beta-galactosidase. In case of the other analogs listed above, the incorporation of even a small fraction of analog into rRNA resulted in drastic inhibition of beta-galactosidase synthesis.
D004926:Escherichia coli / Q000201:enzymology; D005696:Galactosidases / Q000235:genetics*; D005796:Genes; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics; D012265:Ribonucleotides / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic / Q000187:drug effects*; D001616:beta-Galactosidase / Q000235:genetics*
D012333:RNA, Messenger; D012265:Ribonucleotides; D005696:Galactosidases; D001616:beta-Galactosidase