Freeze-fracture study of isolated lysosomes of rat liver.
Freeze-fracture electron microscopy was utilized to pursue the morphological characterization of isolated lysosomes. Combining biochemical data of purity and morphological criteria of identification, it was possible to describe the convex and concave fracture faces of lysosomes. We have compared these with the corresponding faces of tritosomes i.e. lysosomes isolated after injection of Triton WR-1339 into the animal.
D000818:Animals; D005614:Freeze Fracturing / Q000379:methods; D008099:Liver / Q000648:ultrastructure*; D008247:Lysosomes / Q000648:ultrastructure*; D008854:Microscopy, Electron / Q000379:methods