Secondary structures of hyaluronate and chondroitin sulphates. A 1H n.m.r. study of NH signals in dimethyl sulphoxide solution.
1H n.m.r. spectra in [2H6]dimethyl sulphoxide of dodecyltrimethylammonium salts of chondroitin sulphates and hyaluronate, or sodium salts of oligomers from hyaluronate, showed unambiguous NH signals. The acetamido NH occurs in two different environments: environment I ('normal') in simple sugars, and environment II (hydrogen-bonded NH) appearing in tri- or tetrasaccharides, indicating a secondary structure in hyaluronate (and some chondroitin sulphates) involving a hydrogen-bonded acetamido NH.
D002807:Chondroitin / Q000031:analogs & derivatives; D002809:Chondroitin Sulfates; D003902:Detergents; D004121:Dimethyl Sulfoxide; D006820:Hyaluronic Acid; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents
D003902:Detergents; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; D006820:Hyaluronic Acid; D002807:Chondroitin; D002809:Chondroitin Sulfates; C022806:dodecyltrimethylammonium; D004121:Dimethyl Sulfoxide