Demonstration and partial characterization of a 7,12-dimethylbenz[alpha]anthracene-binding protein in rat adrenal.
Rat adrenal cytosol was found to contain a 7,12-dimethylbenz[a]-anthracene (DMBA)-binding protein which is characterized by a pI of 7.2, a Kd-value of 3 microM and a maximal capacity of about 47 pmol/mg protein. The binding is highly specific for DMBA and is not displaced by 3-methylcholanthrene (MC), benz[a]pyrene (BP) or other polycyclic hydrocarbons. Likewise, various androgens, estrogens or glucocorticoids have no effect on the DMBA binding. It is proposed that the DMBA-binding protein may have a role in the toxic effects of DMBA or DMBA metabolites in adrenal and possibly in other DMBA-sensitive organs as well.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000302:isolation & purification / Q000378:metabolism; D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel; D003600:Cytosol / Q000378:metabolism; D006358:Hot Temperature; D007525:Isoelectric Focusing; D008297:Male; D011083:Polycyclic Compounds / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013256:Steroids / Q000494:pharmacology; D014357:Trypsin / Q000378:metabolism
C032223:7,12-dimethylbenzanthracene-binding protein, rat; D002352:Carrier Proteins; D011083:Polycyclic Compounds; D013256:Steroids; D015127:9,10-Dimethyl-1,2-benzanthracene; D014357:Trypsin