title: Detection of externally induced impairments in single bacterial cells by laser microbe mass analysis.
abstract: Applying the laser microbe mass analyzer method (LAMMA), mass spectra (fingerprints) were taken from single bacterial cells not treated or treated with high temperature, X-irradiation, isonicotinic acid hydrazide (INH), or diaminodiphenylsulfone (DDS). Spectra of treated cells ("M. lufu," M. tuberculosis H37Ra, E. coli) differ from those of controls in that the K+/Na+ ratio was smaller and in that the intensities of peaks with m/e greater than 100 were lower. From the results with M. leprae the possible application of this new method for monitoring the effectiveness of leprosy therapy is proposed.
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D003622:Dapsone / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism; D007834:Lasers; D007918:Leprosy / Q000382:microbiology; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D009166:Mycobacterium leprae / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000378:metabolism
chemical_list: D003622:Dapsone