Effect of liposomes containing cholesterol on a hepatic cholesterol-7 a-hydroxylase and drug oxidation system.
A statistically significant increase (p less than 0.01) in the activities of the microsomal enzymes cholesterol-7 a-hydroxylase (EC 1.14.13.17), biphenyl-4-hydroxylase, aniline hydroxylase, NADPH-cytochrome-c-reductase (EC 1.6.2.4) and p-nitroanisole-O-demethylase, was shown in cholesterol-loaded rat liver microsomes. The data indicate that profound functional changes are induced in membrane proteins by exogenous cholesterol.
D000815:Aniline Hydroxylase / Q000378:metabolism; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D002784:Cholesterol / Q000494:pharmacology*; D002790:Cholesterol 7-alpha-Hydroxylase / Q000378:metabolism*; D007700:Kinetics; D008081:Liposomes; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D009575:Nitroanisole O-Demethylase / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D013250:Steroid Hydroxylases / Q000378:metabolism*
D008081:Liposomes; D002784:Cholesterol; D009575:Nitroanisole O-Demethylase; D010088:Oxidoreductases; D013250:Steroid Hydroxylases; D000815:Aniline Hydroxylase; D001189:Aryl Hydrocarbon Hydroxylases; C019793:biphenyl-4-hydroxylase; D002790:Cholesterol 7-alpha-Hydroxylase; D009251:NADPH-Ferrihemoprotein Reductase