[Purification and specificity control of labelled antibodies (author's transl)].
A review is given about causes of unspecific binding and the possibility of its prevention. Unspecific binding can be caused by: binding of proteins and glycoproteins on cells or tissues by non-covalent links, undesirable antigen-antibody-reactions, non-immune affinity reactions, changes of the charge of antibodies depending on the labelling procedure. A method for fractionation of labelled antibodies by using precipitation with protamine sulphate is described.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology
D000906:Antibodies; D007074:Immunoglobulin G
Reinigung und Spezifitätskontrolle von markierten Antikörpen.