Variable effects of indomethacin and four related compounds on lymphocyte blastogenesis and cell-mediated cytotoxicity.
Activation of spleen cell cultures by T-cell mitogens appeared enhanced or suppressed by the presence of some nonsteroid anti-inflammatory drugs such as indomethacin and indomethacin esters in a dose-dependent fashion. However blastogenesis of B cells by bacterial lipopolysaccharide was inhibited by the same drugs at every concentration tested. In vivo treatment of mice with indomethacin led to an inhibition of both T and B lymphocyte responses to mitogens. Addition of indomethacin to Concanavalin A-stimulated spleen cells from indomethacin-treated mice further inhibited the response to the mitogen. Indomethacin and indomethacin esters induced only a slight inhibition of cell-mediated cytotoxic reactions when added to cultures, whereas these reactions were markedly inhibited in spleen cells from indomethacin-treated mice.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004952:Esters; D066298:In Vitro Techniques; D007213:Indomethacin / Q000031:analogs & derivatives* / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D012515:Mast-Cell Sarcoma / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D011453:Prostaglandins / Q000096:biosynthesis; D012513:Sarcoma, Experimental / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects
D004952:Esters; D008070:Lipopolysaccharides; D011453:Prostaglandins; D003208:Concanavalin A; D007213:Indomethacin