Optimal fixation conditions for the immunoperoxidase identification of human J chain from tissue sections.
J chain can be used as a marker of plasmablasts and plasma cells at an earlier stage than intracellular immunoglobulin. Immunoperoxidase techniques were used to study optimal fixation conditions for the preservation of human J chain antigenicity in paraffin-embedded tissue sections. The most constantly positive staining for J chain combined with good morphological integrity was obtained with Bouin's fluid for 1.5 h at 20 degrees C. All other fixatives studied showed less consistent staining results.
D000447:Aldehydes / Q000494:pharmacology*; D001152:Arsenicals / Q000494:pharmacology*; D002101:Cacodylic Acid / Q000494:pharmacology*; D005404:Fixatives; D005770:Gastrointestinal Neoplasms / Q000378:metabolism; D005976:Glutaral / Q000494:pharmacology*; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007144:Immunoglobulin J-Chains / Q000032:analysis*; D013154:Spleen / Q000378:metabolism
D000447:Aldehydes; D001152:Arsenicals; D005404:Fixatives; D007144:Immunoglobulin J-Chains; D002101:Cacodylic Acid; D005976:Glutaral