Control by phospho-adenosinediphospho-ribose of NADP-dependent isocitrate dehydrogenase and 6-phosphogluconate dehydrogenase in Streptomyces griseus.
The metabolic function of NAD(P)-glycohydrolase in the streptomycin-producing Streptomyces griseus was investigated. Phospho-adenosinediphospho-ribose, the product of NAD(P)-glycohydrolase reaction was shown to interfere as a competitive inhibitor not only with the glucose-6-phosphate dehydrogenase (VORONINA et al. 1978) but also with the NADP-dependent isocitrate and 6-phospho-gluconate dehydrogenases. Inhibition kinetics were studied with isocitrate dehydrogenase from pig heart and 6-phosphogluconate dehydrogenase from yeast as well as with mycelial extracts of a mutant of S. griseus lacking NAD(P)-glycohydrolase.
D000246:Adenosine Diphosphate Ribose / Q000031:analogs & derivatives / Q000494:pharmacology*; D002474:Cell-Free System; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D009154:Mutation; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009244:NAD+ Nucleosidase; D009249:NADP / Q000378:metabolism; D009702:Nucleoside Diphosphate Sugars / Q000494:pharmacology*; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics; D013305:Streptomyces griseus / Q000187:drug effects*; D013307:Streptomycin / Q000235:genetics
D009702:Nucleoside Diphosphate Sugars; D009243:NAD; C020857:phosphoadenosine diphosphoribose; D000246:Adenosine Diphosphate Ribose; D009249:NADP; D007521:Isocitrate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D009699:N-Glycosyl Hydrolases; D009244:NAD+ Nucleosidase; D013307:Streptomycin