Electrophoretic patterns of aminoacylase-1 (ACY-1) isozymes in vertebrate cells and histochemical procedure for detecting ACY-1 activity.
A histochemical procedure has been developed for staining aminoacylase-1 (ACY-1) isozymes after electrophoresis on cellulose acetate membranes. N-Formyl-L-methionine and N-acetyl-L-methionine were excellent enzyme substrates in the staining reaction. The ACY-1 isozymes from tissue culture cells of several vertebrate species showed distinguishable electrophoretic patterns. The ACY-1 isozymes in extracts of mouse, human, owl monkey, and African green monkey kidney cells each had electrophoretic mobilities different from those of peptidases S, A, and C from the same cells. Exept for African green monkey kidney (CV-1) cells, the animal species expressed a single anodally migrating ACY-1 band. Human-mouse somatic cell hybrids containing the short arm of human chromosome 3 expressed three ACY-1 bands, as would be predicted from the dimeric structure of the enzyme. CV-1 cells expressed two (or three) ACY-1 bands, suggesting the possibility that CV-1 cells contained two alleles at a single locus or two genetic loci for ACY-1.
D000581:Amidohydrolases / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002645:Chickens; D006224:Cricetinae; D003412:Cricetulus; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D000882:Haplorhini; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism*; D007668:Kidney; D008168:Lung; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D010053:Ovary; D051381:Rats; D013045:Species Specificity
D007527:Isoenzymes; D000581:Amidohydrolases; C019672:aminoacylase I