The effects of a monoclonal anti-mu chain antiserum on the in vitro growth of normal B cells.
Membrane immunoglobulin (Ig) on B cells plays a role in specifically focusing antigen onto the cell surface. An unresolved question is whether the Ig receptor itself has the capacity to deliver a signal to the B cell, and if so what is the mechanism of that signal. To explore these questions we have developed a monoclonal rat anti-mouse mu chain antibody (E4). In this study it is shown that protein A-Sepharose-purified monoclonal anti-mu chain antibody inhibits proliferation of normal B cells in vitro even at the single cell level. This effect was independent of Fc receptor interactions. Furthermore, E4 was shown to inhibit lipopolysaccharide-induced mitogenesis. These results provide evidence that interaction of antibody with only a single determinant on the Ig receptor can deliver a negative signal to the B cell.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D007143:Immunoglobulin Heavy Chains / Q000276:immunology*; D007148:Immunoglobulin mu-Chains / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology; D051381:Rats; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*; D011961:Receptors, Fc / Q000276:immunology; D011971:Receptors, Immunologic / Q000276:immunology; D013154:Spleen / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007143:Immunoglobulin Heavy Chains; D007148:Immunoglobulin mu-Chains; D011947:Receptors, Antigen, B-Cell; D011961:Receptors, Fc; D011971:Receptors, Immunologic