Electron microscopic demonstration of negative surface charges on cytoplasmic membranes of Bacillus subtilis with protamine-ferritin.
The application of a protamine-ferritin conjugate for labelling of isolated protoplast membranes of Bacillus subtilis S 13/1 is described. Contrary to Mycoplasma membranes which could only be labelled on the outer side of the membrane, ferritin was deposited on both membrane sides as a single layer without cluster formation.
D001412:Bacillus subtilis / Q000032:analysis* / Q000648:ultrastructure; D002462:Cell Membrane / Q000032:analysis; D005293:Ferritins / Q000378:metabolism; D011108:Polymers / Q000378:metabolism; D011479:Protamines; D011523:Protoplasts / Q000032:analysis; D013499:Surface Properties
D011108:Polymers; D011479:Protamines; C010297:polycationic ferritin; D005293:Ferritins