A solid phase fluoroimmunoassay of serum cortisol.
Direct solid phase fluoroimmunoassay of serum cortisol was established by using fluorescein isothiocyanate labelled anti-cortisol antibody and cortisol conjugated polyacrylamide beads. Sodium salicylate was used as a blocking agent for cortisol binding proteins. The sensitivity of this assay was 0.2 ng/assay tube and the measurable range was from 2 to 100 micrograms/dl using 10 microliters of serum. Intra- and inter-assay coefficients of variation were 11.6% (mean +/- SD 19.8 +/- 2.3 micrograms/dl, n = 5): and 12.1% (mean +/- SD 22.8 +/- 2.8 micrograms/dl, n = 5), respectively. THe accuracy was estimated from recovery study and the average recovery was 100.2%. Cortisol values determined by the present method correlated well with those determined by radioimmunoassay (r = 0.98, y = 0.98 x + 0.04, n = 35). The proposed assay satisfied the standard criteria of dilution, accuracy, and precision, and is applicable to routine measurement of serum cortisol.
D002352:Carrier Proteins / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D008832:Microchemistry / Q000379:methods; D011863:Radioimmunoassay; D012980:Sodium Salicylate / Q000494:pharmacology; D013861:Thiocyanates
D002352:Carrier Proteins; D005452:Fluoresceins; D013861:Thiocyanates; C027091:cortisol binding globulin; D016650:Fluorescein-5-isothiocyanate; D006854:Hydrocortisone; D012980:Sodium Salicylate