Detection of polysaccharide cell wall antigen of Neisseria gonorrhoeae in a rabbit model by counterimmunoelectrophoresis.
A rabbit chamber model was developed and inoculated with 10(9) colony-forming units (cfu) of viable Neisseria gonorrhoeae to determine whether the lipopolysaccharide-derived Gc2 polysaccharide cell wall antigens could be detected by counterimmunoelectrophoresis (CIE). Four hours after inoculation, a polymorphonuclear leukocyte response was noted in the chambers; this response was followed by progressive phagocytosis of the organisms and a fall in number of cfu/ml. All visible bacteria were intracellular, and chamber fluids were sterile 6 hr after inoculation. Use of sero specific antisera permitted detection by CIE of the Gc2 polysaccharide antigen in sera of all rabbits within 48 hr after inoculation of the chambers, whereas blood cultures remained sterile throughout the experiment. At 2-6 hr after inoculation, the Gc2 polysaccharide antigen was also detected as a single precipitin band in the chamber fluid of inoculated rabbits. At 24 hr the precipitin band was not observed; rather, a halo above the antigen well was noted. The halo was found to be a nonspecific complex containing the Gc2 polysaccharide antigen and no antibody. In the rabbit model studied, CIE was sufficiently sensitive to detect concentrations of the Gc2 polysaccharide antigen of greater than or equal to 0.97 microgram/ml in serum and chamber fluid.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D002473:Cell Wall / Q000276:immunology; D003378:Counterimmunoelectrophoresis / Q000379:methods*; D004195:Disease Models, Animal; D005260:Female; D006069:Gonorrhea / Q000276:immunology; D007122:Immunoelectrophoresis / Q000379:methods*; D009344:Neisseria gonorrhoeae / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D011817:Rabbits; D013997:Time Factors
D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial