In vivo inhibition of incorporation of [U-14C]glucose into proteins in experimental focal epilepsy.
The in vivo incorporation of [14C] from [U-14C]-glucose into rat brain proteins from different cortical areas was examined in three different experimental focal epilepsies: cobalt, freeze-lesions, and tityustoxin. When [U-14C]-glucose was injected intraperitoneally into awake and unrestrained animals with marked signs of epileptic hyperactivity, the inhibition of incorporation of [14C]-amino acids into trichloracetic acid (TCA)-insoluble proteins was highest in the focal (sensorimotor) area when compared with distant regions (approx. 60%), but less when compared with the contralateral (sensorimotor) region (approx. 23%). Greatly decreased incorporation caused by both cobalt and freeze-lesion-induced epilepsies was also observed in the contralateral area when a comparison was made with distant regions (approx. 50%), but there were no significant differences in protein-specific radioactivity between the different distant areas.
D000818:Animals; D002250:Carbon Radioisotopes / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D003035:Cobalt; D004828:Epilepsies, Partial / Q000378:metabolism; D005260:Female; D005615:Freezing; D005947:Glucose / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012604:Scorpion Venoms; D012640:Seizures / Q000209:etiology / Q000378:metabolism*; D014238:Trichloroacetic Acid / Q000378:metabolism
D002250:Carbon Radioisotopes; D009419:Nerve Tissue Proteins; D012604:Scorpion Venoms; C003609:tityustoxin; D003035:Cobalt; D014238:Trichloroacetic Acid; D005947:Glucose