Effects of gold sodium thiomalate on cytosolic copper and zinc in the rat kidney and liver tissues.
1. Male Wistar rats were given a single subcutaneous injection of gold sodium thiomalate and killed 7 days later. The binding of Au, Zn and Cu to the kidney and liver cytosolic proteins of control and gold-treated rats and determined. 2. In the renal cytosol of Au(I)- exposed rats, the binding of Cu to the low-molecular-weight (LMW) proteins increased by 62%, and to the high-molecular-weight (HMW) proteins the binding decreased by 54%. The incorporation of Cu into the liver cytosolic proteins increased, in both, the HMW and the LMW proteins. The binding of Zn into the renal cytosolic proteins was increased by 39% (HMW proteins) and 100% (LMW proteins). Au(I) had little effect on the binding of Zn to the cytosolic proteins in the liver. 3. It is suggested that the therapeutic action of gold complexes may be mediated, to some extent, by its effects on the metabolism of Cu and/or Zn.
D000818:Animals; D002850:Chromatography, Gel; D003300:Copper / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D006052:Gold Sodium Thiomalate / Q000378:metabolism / Q000494:pharmacology*; D007279:Injections, Subcutaneous; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008668:Metallothionein / Q000378:metabolism; D008970:Molecular Weight; D011485:Protein Binding / Q000187:drug effects; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015032:Zinc / Q000378:metabolism*
D011506:Proteins; D006052:Gold Sodium Thiomalate; D003300:Copper; D008668:Metallothionein; D015032:Zinc