title: Thin layer chromatographic determination of aflatoxin in corn dust.
abstract: Methods adopted by the AOAC and the American Association of Cereal Chemists for determining aflatoxin in corn were modified, and techniques were developed for application to samples of less than 1 to 10 g instead of the specified 50 g samples. Analysis included chloroform extraction of dust samples or dust collected from glass fiber filters, purification of extracts on a silica gel column of appropriate size, and measurement of aflatoxin by either 1- or 2-dimensional thin layer chromatography (TLC). The solvent for 1-dimensional TLC was chloroform-acetone-water (91 + 9 + 1). Solvents for 2-dimensional TLC were, first direction, ether-methanol-water (95 + 4 + 1, lined tank) and second direction, chloroform-acetone-water (91 + 9 + 1, unlined tank), or first direction, chloroform-acetone-water (91 + 9 + 1, unlined tank) and second direction, toluene-ethyl acetate-formic acid (60 + 30 + 10, unlined tank). When samples weighed less than or equal to 0.1 g, the entire concentrated extract was applied to the TLC plate. About 0.5-1.0 ng aflatoxin B1 could be detected on the plate, making the limit of detection about 9 ng/g for 0.1 g samples.
mesh_terms: D016604:Aflatoxin B1; D000348:Aflatoxins / Q000032:analysis*; D002845:Chromatography / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D004391:Dust / Q000032:analysis*; D003313:Zea mays / Q000032:analysis*
chemical_list: D000348:Aflatoxins; D004391:Dust; D016604:Aflatoxin B1