Increased incorporation of [3H]lysine into synaptic membranes of the visual cortex following first exposure of dark-reared rats to light is confined to particular polypeptides.
The incorporation of [3H]lysine into separated polypeptides of synaptic-membrane fractions prepared from the visual cortices of dark-reared rats and littermates exposed to light for 1 h was examined. Increased incorporation of [3H]lysine was found in synaptic membranes from light-exposed compared to dark-reared rats in polypeptides of four molecular weights: 100,000, 71,000, 44,000, and 38,000. The 44,000-molecular-weight peak has been suggested to be actin on the basis of its comigration with pure brain actin. These results indicate that increased incorporation of [3H]lysine into synaptic membranes of the visual cortex, following first exposure of dark-reared rats to light, is confined to particular polypeptides.
D000818:Animals; D003624:Darkness; D004591:Electrophoresis, Polyacrylamide Gel; D008239:Lysine / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D010455:Peptides / Q000378:metabolism*; D010775:Photic Stimulation; D051381:Rats; D013570:Synaptic Membranes / Q000378:metabolism*; D014793:Visual Cortex / Q000378:metabolism*
D010455:Peptides; D008239:Lysine