Distribution of protein I in mammalian brain as determined by a detergent-based radioimmunoassay.
A radioimmunoassay has been developed for measuring protein I, a basic, neuron-specific protein associated with nerve terminals. The procedure utilizes the detergents NaDodSO4 and Nonidet P-40 to prevent nonspecific adsorption of this highly charged protein to various surfaces. By use of this procedure, it has been possible to show that protein I comprises approximately 0.4% of the total protein in cerebral cortex of several mammalian species. In addition, the amount of protein I was determined in about 40 regions of cat brain. The results suggest that measurement of protein I may provide a quantitative method for estimating the density of nerve terminals in various regions of  the mammalian nervous system.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D001931:Brain Mapping; D002415:Cats; D002417:Cattle; D002540:Cerebral Cortex / Q000378:metabolism; D003902:Detergents; D006801:Humans; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D016704:Synapsins
D003902:Detergents; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D016704:Synapsins