Improvements in the passive immune hemolysis test for assaying enterotoxigenic Escherichia coli.
The sensitivity of the passive immune hemolysis test for the detection of heat-labile enterotoxin from Escherichia coli was increased when the test was carried out with Veronal-buffered saline plus Ca2+ and Mg2+ as diluent, instead of phosphate-buffered saline. The performance of the test was further improved by using stationary cultures to which mitomycin C had been added at the end of the lag phase.
D000311:Adrenal Glands; D001427:Bacterial Toxins; D001681:Biological Assay; D002118:Calcium; D002460:Cell Line; D004768:Enterotoxins / Q000032:analysis*; D004926:Escherichia coli / Q000032:analysis* / Q000254:growth & development; D029968:Escherichia coli Proteins; D006462:Hemolytic Plaque Technique; D008274:Magnesium; D016685:Mitomycin; D008937:Mitomycins
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D008937:Mitomycins; D016685:Mitomycin; C027538:heat-labile enterotoxin, E coli; D008274:Magnesium; D002118:Calcium