Heat-denaturation of pig kidney diamine oxidase: effect of arcaine.
Pig kidney diamine oxidase is protected against heat denaturation by the presence of inhibitors like tetramethylene diguanine (arcaine). In the presence of inhibitor the enzyme retains its full activity even after 30 min. incubation at 60 degrees C. This inhibitor binds to the enzyme inducing small but significant absorption changes in the near uv spectrum. Temperature dependence of intrinsic fluorescence yield is different in the presence and absence of arcaine, indicating a conformational stabilizing effect of this inhibitor.
D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism*; D000818:Animals; D001645:Biguanides / Q000494:pharmacology*; D005453:Fluorescence; D006358:Hot Temperature; D007672:Kidney Cortex / Q000201:enzymology*; D011489:Protein Denaturation; D013056:Spectrophotometry, Ultraviolet; D013552:Swine
D001645:Biguanides; C006624:arcaine; D006631:Amine Oxidase (Copper-Containing)