Effects of amine substances on susceptibility of cells to agglutination by concanavalin A and on paracrystal formation by vinblastine in untransformed 3T3 cells.
The effects of a secondary amine (ketamine), tertiary amines (dibucaine, lidocaine, marcaine, propanidid, diazepam and chlorpromazine) and a quaternary amine (tetraethylammonium bromide, TEA) on mouse 3T3 cell agglutination by concanavalin A (Con A), on patch formation of Con A receptors on the cell surface, and on paracrystal formation by vinblastine in cytoplasm were studied. These amines enhanced the cell agglutination at low concentrations of Con A, as did the mixture of colchicine and cytochalasin B. Ca++, applied extracellularly, inhibited the effects of these amines on cell agglutination by Con A. The patch formation of Con A receptors on the cell surface as revealed by fluoresceinated Con A was enhanced by these amines. Ketamine, dibucaine and TEA inhibited the paracrystal formation in cytoplasm, as did Ca++ ionophores such as A-23187 and X-537-A. These results suggest that the amines tested affect the fluidity of Con A receptors by impairment of cell membrane structural proteins and result in the increase of the susceptibility of cells to agglutination by Con A.
D000371:Agglutination / Q000187:drug effects*; D000588:Amines / Q000494:pharmacology*; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D003460:Crystallization; D007832:Lasalocid / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011952:Receptors, Concanavalin A / Q000032:analysis; D013329:Structure-Activity Relationship; D014747:Vinblastine / Q000494:pharmacology*
D000588:Amines; D011952:Receptors, Concanavalin A; D003208:Concanavalin A; D000001:Calcimycin; D014747:Vinblastine; D007832:Lasalocid