[Effect of preincubation of hepatic microsomes in the presence of chloramphenicol on microsomal lipid peroxidation].
The influence of anaerobic preincubation of hepatic microsomes in the presence of chloramphenicol, in the view of reduction of nitro-group has been studied on later peroxidative degradation and activity of several enzymatic systems on lipids of pretreated microsomes. An inhibition of malonaldehyde production and conjugated dienes occurs in these conditions. Activity of methylaniline N-demethylase and Neotetrazolium reductase were not affected. Possible relationships of chloramphenicol and its reduced metabolites on lipoperoxidation are discussed.
D000693:Anaerobiosis; D000818:Animals; D002701:Chloramphenicol / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D008054:Lipid Peroxides; D008315:Malondialdehyde; D002701:Chloramphenicol; D010089:Oxidoreductases, N-Demethylating; C021848:NADPH-tetrazolium reductase; D009251:NADPH-Ferrihemoprotein Reductase
Effects d'une préincubation anaérobie de microsomes hépatiques on présence de chloramphénicol sur la péroxydation des lipides microsomaux.