Light-chain profiles in sera from patients with subacute sclerosing panencephalitis.
Sera from 2 subacute sclerosing panencephalitis (SSPE) patients were absorbed with a concentrated preparation of measles virus. Measles-specific IgG was eluted from the precipitates containing measles antigen-antibody complex. These IgGs, when subjected to immunofixation after isoelectric focusing showed a number of oligoclonal bands with one type of light-(L) chain. In urea polyacrylamide gel electrophoresis, the reduced and alkylated measles-specific IgG showed 1-3 homogeneous L-chain bands, whereas IgG isolated from unabsorbed sera and IgG isolated from supernatants of SSPE sera after absorption with measles virus showed a diffuse L-chain band. It can be concluded that in SSPE, measles virus is responsible for the synthesis of L-chain with restricted heterogeneity.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000134:cerebrospinal fluid; D002999:Clone Cells / Q000276:immunology; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis* / Q000145:classification; D007525:Isoelectric Focusing; D008459:Measles virus / Q000276:immunology; D013344:Subacute Sclerosing Panencephalitis / Q000209:etiology / Q000276:immunology*
D000914:Antibodies, Viral; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains