title: Mutagenesis during transformation of Bacillus subtilis. II. An increase in chemically-induced mutations during competency.
abstract: During the development of competency in Bacillus subtilis there was an increased sensitivity to methyl methanesulfonate (MMS) treatments. The frequency of reverse mutation also increased among the MMS-revertible markers by a factor of 100 as compared to vegetative cultures. The frequency of 2-aminopurine(AP)-induced mutagenesis was the same in competent and noncompetent cultures. Studies with DNA-polymerase-deficient mutants showed a direct involvement of DNA polymerase I in promoting MMS and transformation-induced mutagenesis in competent cells.
mesh_terms: D015075:2-Aminopurine / Q000494:pharmacology; D001412:Bacillus subtilis / Q000235:genetics*; D004256:DNA Polymerase I / Q000235:genetics*; D004269:DNA, Bacterial; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D005787:Gene Frequency; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D009154:Mutation / Q000187:drug effects; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial; D015075:2-Aminopurine; D008741:Methyl Methanesulfonate; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase