Colon-specific antigen-p (CSAp). II. Further characterization in colorectal and pancreatic cancer.
The physicochemical and immunological characteristics of colon-specific antigen-p (CSAp) in plasma and in colorectal and pancreatic tumors were investigated. CSAp in the plasma of a rectal cancer patient and in a colonic carcinoma xenografted in hamsters (GW-39 tumor) appeared to have similar chromatographic properties, being of a molecular size of 4 million or more. The activities of CSAp in both plasma and tumor were similarly destroyed by treatment with a thiol reagent. Finally, identical immunological reactions in radioimmunoassay and gel diffusion tests were obtained between the CSAp's in patient plasma and in GW-39 tumor tissue. Also CSAp in human pancreatic cancers xenografted in nude mice showed a precipitin line of complete identity with CSAp extracted from GW-39 human colonic carcinoma transplants. Thus, the CSAp's found in colorectal cancer patient plasma, in colonic carcinoma, and in pancreatic cancer tissues appear to be immunologically identical.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D002460:Cell Line; D002850:Chromatography, Gel; D003110:Colonic Neoplasms / Q000276:immunology*; D006224:Cricetinae; D006801:Humans; D005779:Immunodiffusion; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology; D010190:Pancreatic Neoplasms / Q000276:immunology*; D011863:Radioimmunoassay; D012004:Rectal Neoplasms / Q000276:immunology*
D000951:Antigens, Neoplasm; C030139:colon-specific antigen