Production of amino acids by analog-resistant mutants of the cyanobacterium Spirulina platensis.
Mutants of Spirulina platensis resistant to 5-fluorotryptophan, beta-3-thienyl-alanine, ethionine, p-fluorophenylalanine, or azetidine-2-carboxylic acid were isolated. Some of these mutants appeared to be resistant to more than one analog and to overproduce the corresponding amino acids. A second group was composed of mutants that were resistant to one analog only. Of the latter mutants, one resistant to azetidine-2-carboxylic acid was found to overproduce proline only, whereas one resistant to fluorotryptophan and one resistant to ethionine did not overproduce any of the tested amino acids.
D000409:Alanine / Q000031:analogs & derivatives / Q000494:pharmacology; D001383:Azetidinecarboxylic Acid / Q000494:pharmacology; D000458:Cyanobacteria / Q000187:drug effects / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D005001:Ethionine / Q000494:pharmacology; D008715:Methionine / Q000096:biosynthesis*; D010649:Phenylalanine / Q000096:biosynthesis*; D011392:Proline / Q000096:biosynthesis*; D014364:Tryptophan / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000494:pharmacology; D010135:p-Fluorophenylalanine / Q000494:pharmacology
C100177:2-thienylalanine; C026927:5-fluorotryptophan; D010649:Phenylalanine; D001383:Azetidinecarboxylic Acid; D010135:p-Fluorophenylalanine; D014364:Tryptophan; D011392:Proline; D008715:Methionine; D000409:Alanine; D005001:Ethionine