[Relation between the results of gel filtration and electrophoresis of serotype A and G meningococcal polysaccharides].
Meningococcal polysaccharides, serogroups A and C, produced for the vaccinal prophylaxis of meningococcal infection, were found to have pronounced electrophoretic homogeneity in their movement towards the anode in the process of immunoelectrophoresis in agarose gel with pH 8.6. In gel filtration through Sepharose 4B the serologically active substance of meningococcal polysaccharides, groups A and C, was eluted in a wide range of Kd values from 0 to 0.9. The immunoelectrophoresis of the samples isolated in gel filtration and having definite Kd values indicated that the regular increase of electrophoretic mobility towards anode occurred as the size or weight of molecules decreased.
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D007123:Immunoelectrophoresis, Two-Dimensional; D008970:Molecular Weight; D009345:Neisseria meningitidis / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification*
D011135:Polysaccharides, Bacterial
Sootnoshenie pokazateleĭ gel'-filtratsii i élektroforeza meningokokkovykh polisakharidov serogrupp A i C.