title: Cytoplasmic crystalloids in irradiated rat parotid glands.
abstract: Cytoplasmic crystalloids were found in parotid acinar cells of rats given a large (6400 R) single exposure of X-rays to the head and neck. The crystalloids were first observed 1 day after irradiation and became numerous at 3-4 days. They were associated with autophagic vacuoles, which were seen in acinar cells as early as 3-6 h. Crystalloids sometimes appeared to be forming within autophagic vacuoles, which also contained membranous residues and apparently degenerating secretory material. They were bounded by a single smooth membrane and had a substructure consisting of dense, parallel longitudinal striations. They crystalloids were also seen in macrophages associated with the basal surface of acinar cells. At 3-4 days macrophages were numerous and many contained crystalloids, degenerated secretory droplets, and other cellular debris, which they apparently had phagocytosed. By 6-8 days crystalloids and macrophages were seen infrequently. Regarding mode of formation, removal by macrophages, and ultrastructure, the crystalloids resembled those described by others after ethionine intoxication. Ethionine-induced crystalloids have cytochemical characteristics consistent with a lysosomal identity. The crystalloids in irradiated parotid glands probably reflect a variant type of lysosome, which is a nonspecific manifestation of severe cellular injury and can be elicited by a variety of injurious agents.
mesh_terms: D000818:Animals; D003460:Crystallization; D003594:Cytoplasmic Granules / Q000528:radiation effects* / Q000648:ultrastructure; D005260:Female; D008264:Macrophages / Q000528:radiation effects / Q000648:ultrastructure; D010306:Parotid Gland / Q000528:radiation effects* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors