Lyt-2-/Lyt 3- variants of a cloned cytolytic T cell line lack an antigen receptor functional in cytolysis.
To investigate the role of Lyt-2 and Thy-1 in cytolysis, we have generated, by ethyl methanesulfonate mutagenesis and selection, variants of the cloned cytolytic T lymphocyte line L3 that specifically lack either Lyt-2 or Thy-1. An analysis of these variants indicates that neither Lyt-2 nor Lyt-3 is responsible for the lethal hit, but suggests that Lyt-2 and/or Lyt-3 are required for an antigen receptor functional in cytolysis. The data also suggest that the expression of Lyt-3 on the cell surface is not independent of the expression of Lyt-2. Finally the data indicate the Thy-1 plays no role in cytolysis.
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D001667:Binding, Competitive; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005260:Female; D014644:Genetic Variation; D007519:Isoantigens; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010641:Phenotype; D051381:Rats; D011917:Rats, Inbred Lew; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007519:Isoantigens; D011948:Receptors, Antigen, T-Cell; D003208:Concanavalin A; D005020:Ethyl Methanesulfonate