title: Artificial infection of Rhipicephalus appendiculatus with Theileria parva by percutaneous injection.
abstract: Rhipicephalus appendiculatus nymphs were inoculated with fresh or cryopreserved blood containing Theileria parva piroplasms, or with cell culture grown stages of T parva. The use of fresh blood was successful. Cryopreserved blood containing dimethylsulphoxide (DMSO), killed most nymphs after inoculation: DMSO could be removed by slow dialysis, without destroying the infectivity of the blood. Attempts to infect ticks by inoculating cell culture grown stages of T parva failed, even when large numbers of merozoites were present in the inoculum.
mesh_terms: D000818:Animals; D016782:Apicomplexa / Q000254:growth & development*; D001769:Blood / Q000469:parasitology; D001793:Blood Preservation / Q000662:veterinary; D002417:Cattle; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005260:Female; D005615:Freezing; D008297:Male; D009758:Nymph / Q000469:parasitology; D013801:Theileriasis / Q000469:parasitology* / Q000635:transmission; D013987:Ticks / Q000469:parasitology*
chemical_list: D004121:Dimethyl Sulfoxide