Inhibition of lysosomal alpha-mannosidase by swainsonine, an indolizidine alkaloid isolated from Swainsona canescens.
An indolizidine alkaloid (swainsonine) was isolated from the plant Swainsona canescens. Swainsonine is a specific and potent inhibitor of alpha-mannosidase (EC 3.2.1.24) and when administered to animals produces a phenocopy of the genetically based lysosomal storage disease, mannosidosis. Evidence is presented to suggest that swainsonine is a reversible active site-directed inhibitor of lysosomal alpha-mannosidase.
D000470:Alkaloids / Q000494:pharmacology*; D006867:Hydrolases / Q000037:antagonists & inhibitors; D007700:Kinetics; D008247:Lysosomes / Q000201:enzymology; D008361:Mannosidases / Q000037:antagonists & inhibitors*; D010947:Plants, Toxic / Q000032:analysis*; D017026:Swainsonine
D000470:Alkaloids; D006867:Hydrolases; D008361:Mannosidases; D017026:Swainsonine