Inactivation of transforming Bacillus subtilis deoxyribonucleic acid by monoadducts of 4,5',8-trimethylpsoralen.
4,5',8-Trimethylpsoralen (TMP) monoadducts inactive transforming deoxyribonucleic acid (DNA) in Bacillus subtilis. Contrary to TMP diadducts (TMP cross-links), which severely inhibit entry of donor DNA (G. Venema and U. Canosi, Mol. Gen. Genet. 179:1--11), TMP monoadducts have only a slight effect on entry. Since reextracted TMP-monoadduct-containing transforming DNA is a differentially repaired by Uvr- and Uvr+ recipients and cross-linkable to the recipient strand in the heteroduplex recombinant DNA molecules, the monoadducts can be integrated along with the donor DNA into the recipient chromosome.
D001412:Bacillus subtilis / Q000235:genetics*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics* / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D011564:Furocoumarins / Q000494:pharmacology*; D008854:Microscopy, Electron; D009691:Nucleic Acid Denaturation; D010777:Photochemistry; D014170:Transformation, Genetic / Q000187:drug effects*; D014307:Trioxsalen / Q000494:pharmacology*; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D011564:Furocoumarins; D014307:Trioxsalen