Radioimmunoassay of human muscle carbonic anhydrase III in dystrophic states.
A radioimmunoassay for the human isozyme carbonic anhydrase III (CAIII) has been developed. The assay can detect levels as low as 4 microgram/l of sample. Plasma CAIII levels in patients suffering from Duchenne muscular dystrophy were found to be up to 39 times greater than levels in a control group. Urine CAIII levels in patients suffering from Duchenne muscular dystrophy were not significantly different from the levels found in urine from normal adults. Measurement of plasma CAIII levels may be useful in prenatal diagnosis of Duchenne muscular dystrophy, and in investigation of adult skeletal muscle disease.
D000818:Animals; D002256:Carbonic Anhydrases / Q000032:analysis*; D006801:Humans; D007093:Imidazoles; D009132:Muscles / Q000201:enzymology*; D009136:Muscular Dystrophies / Q000201:enzymology*; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D014508:Urea / Q000031:analogs & derivatives
D007093:Imidazoles; C012385:1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril; D014508:Urea; D002256:Carbonic Anhydrases