Nucleotide sequence of valine tRNA mo5UAC from bacillus subtilis.
A valine tRNA was purified from Bacillus subtilis W168 by a combined use of several column chromatographic systems. The nucleotide sequence was determined to be pG-G-A-G-G-A-U-U-A-G-C-U-C-A-G-C-D-G-G-G-A-G-A-G-C-A-U-C--G-C-C-U-mo5U-A-C-m6-A -A-G-C-A-G-A-G-G-m7G-U-C-G-G-C-G-G-T-psi-C-G-A-G-C-C-C-G-U-C-A-U-C-C-U-C-C-A-C- C-AOH with the main use of of non-labeled tRNA and with the subsidiary use of [32P]-post-labeled sample. This tRNA contains 5-methoxy-uridine (mo5U) at the wobble position of the anticodon. A binding experiment of valyl-tRNA to ribosome revealed that mo5U is recognized by A and G, and fairly well by U.
D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D003062:Codon; D009690:Nucleic Acid Conformation; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics*; D014529:Uridine / Q000031:analogs & derivatives / Q000032:analysis
D003062:Codon; D012346:RNA, Transfer, Amino Acyl; C016562:5-methoxyuridine; D014529:Uridine