[Preparation of highly potent lyophilized tetanus antitoxin (author's transl)].
The neutralizing antibody titre of tetanus antitoxin could be elevated 3.19 times by the purification of the antitoxin with pepsin digestion method, which is superior to ammonium sulfate salting out method by 1.5 times. But the antitoxin recovery rate by the pepsin digestion method was about 65.37% of the salting out method. Addition of 2% glycine significantly improved the solubility of lyophilized antitoxin. The effective period of liquid antitoxin was 1 year, while that of lyophilized products could be as long as 5 years.
D005612:Freeze Drying; D010434:Pepsin A; D013743:Tetanus Antitoxin / Q000302:isolation & purification
D013743:Tetanus Antitoxin; D010434:Pepsin A