Rapid and sensitive immunochemical determination of immunoglobulins by centrifugal analysis.
The measurement of immunoglobulin concentrations in serum by a turbidimetric method using a centrifugal analyser (Cobas Bio) is described. In the procedure described it is possible to measure the absorbance of each sample in the buffer reagent in a preliminary run without antiserum (sample blank for correction of colour, turbidity, etc.) In the main run (after adding antiserum) a final measurement is made after 600 seconds reaction time and the difference between the final absorbance and the sample blank is corrected for the absorbance of the antiserum determined in the antiserum blank cuvet. The initial phase of the reaction can thus be taken into account so that high titre antisera can be used. Therefore the method covers a wide range of values with good precision.
D000936:Antigen-Antibody Complex; D002498:Centrifugation; D006801:Humans; D005779:Immunodiffusion; D007136:Immunoglobulins / Q000032:analysis*; D009391:Nephelometry and Turbidimetry / Q000379:methods*
D000936:Antigen-Antibody Complex; D007136:Immunoglobulins