Mutagen-induced sister chromatid exchange rate in Bloom syndrome remains unaltered in the presence of Bloom corrective factor.
Fibroblasts of a patient with Bloom syndrome (GM-1492) were cultured in the presence of either mitomycin C, ethylmethanesulfonate, or 4-nitroquinoline-1-oxide, (4-NQ1-O) and sister chromatid exchange was determined. The mutagens enhanced the sister chromatid exchange rate to different degrees, 4-NQ1-O being the most potent substance. Bloom corrective factor, which is present in normal cell-conditioned culture medium, reduced the spontaneously increased SCE in Bloom syndrome cells by about 20 SCE per metaphase but failed to reduce the additional mutagen-induced SCE increase. These findings indicate that only spontaneously, but not mutagen-induced, SCE in Bloom syndrome fibroblasts can be decreased by the Bloom corrective factor.
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000328:Adult; D001816:Bloom Syndrome / Q000235:genetics*; D002460:Cell Line; D002478:Cells, Cultured; D003434:Crossing Over, Genetic / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology*; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005260:Female; D006801:Humans; D008297:Male; D008937:Mitomycins / Q000494:pharmacology; D012854:Sister Chromatid Exchange / Q000187:drug effects; D012867:Skin / Q000648:ultrastructure
D003470:Culture Media; D008937:Mitomycins; D015112:4-Nitroquinoline-1-oxide; D005020:Ethyl Methanesulfonate