Evidence for the enzymic degradation of TRH-related pseudo-peptides by porcine serum and brain homogenate.
Pseudo-peptides (pGlu-His-amphetamine, pGlu-His-pro-Amphetamine and pGlu-amphetamine) were subjected to enzymatic degradation by porcine serum and brain homogenate. The enzymic digests were (qualitatively) analyzed by the "Finger-prints" technique to provide evidence for the presence of free pyroglutamic acid and also quantitatively by HPLC to provide evidence for the presence and yield of free amphetamine. The stability in vitro toward enzymes of serum and brain homogenate of a new type of drug based on the combination of a peptide moiety (TRH) with a non peptide psycho-tropic moiety (amphetamine) is also considered and discussed.
D000662:Amphetamines / Q000097:blood / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D006868:Hydrolysis; D011761:Pyrrolidonecarboxylic Acid / Q000032:analysis; D013552:Swine; D013973:Thyrotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism
D000662:Amphetamines; D013973:Thyrotropin-Releasing Hormone; D011761:Pyrrolidonecarboxylic Acid