Reactivation of chicken erythrocyte nuclei in heterokaryons results in expression of adult chicken globin genes.
Activation of chicken globin gene transcription has been demonstrated in chicken erythrocyte--rat L6 myoblast heterokaryons. The globin mRNA is polyadenylylated and is translated into adult chicken alpha A-, alpha D-, and beta-globin polypeptides. No fetal globin mRNA or globin polypeptides were detected. Heterokaryons between chicken erythrocytes and mouse neuroblastoma cells or hamster BHK cells also synthesized adult chicken globins.
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D002642:Chick Embryo; D002645:Chickens; D002999:Clone Cells; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005796:Genes; D005914:Globins / Q000235:genetics*; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009132:Muscles; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014158:Transcription, Genetic
D008937:Mitomycins; D012333:RNA, Messenger; D016685:Mitomycin; D005914:Globins