Characterization of ecdysteroid receptors in cytosol and naive nuclear preparations of Drosophila Kc cells.
Significant ecdysteroid binding activity can be demonstrated in nuclear extracts obtained from hormonally naive Drosophila Kc cells. The kinetic and physical characteristics of this nuclear binding are presented and compared with those exhibited by a high speed cytosol preparation of Kc cells. Examination of the effect of in vivo ecdysteroid exposure on the number of nuclear binding sites revealed that the quantity of nuclear receptors was not detectably altered. In addition, an effective synthesis of the biologically active ecdysteroid radioligand, [3H]ponasterone A, is described.
D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004331:Drosophila melanogaster / Q000378:metabolism*; D004441:Ecdysterone / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000378:metabolism; D004625:Embryo, Nonmammalian; D007700:Kinetics; D011987:Receptors, Steroid / Q000302:isolation & purification / Q000378:metabolism*
D011987:Receptors, Steroid; C034808:ecdysteroid receptor; D004441:Ecdysterone; C008999:ponasterone A