Electron microscopic localization of factor-VIII-related antigen in adult human blood vessels.
We have localized factor-VIII-related antigen, using immunofluorescence and electron microscopy, in adult human blood vessels. In addition to its presence in endothelial cells, the antigen was localized within subendothelium and the layers of elastic lamina closest to the lumen. Also, we provide the first morphological evidence that factor-VIII-related antigen is associated with collagen fibrils within the vessel wall. These studies suggest that this subendothelial factor-VIII-related antigen may play a role in the adhesion of platelets to subendothelial components following endothelial injury.
D000818:Animals; D000941:Antigens; D001808:Blood Vessels / Q000276:immunology* / Q000648:ultrastructure; D004727:Endothelium / Q000276:immunology / Q000648:ultrastructure; D005169:Factor VIII / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008854:Microscopy, Electron; D011817:Rabbits; D012501:Saphenous Vein / Q000276:immunology / Q000648:ultrastructure; D013157:Splenic Artery / Q000276:immunology / Q000648:ultrastructure; D014471:Umbilical Veins / Q000276:immunology / Q000648:ultrastructure; D014842:von Willebrand Diseases / Q000276:immunology; D014841:von Willebrand Factor
D000941:Antigens; D014841:von Willebrand Factor; D005169:Factor VIII