Identification of Bacillus subtilis men mutants which lack O-succinylbenzoyl-coenzyme A synthetase and dihydroxynaphthoate synthase.
Menaquinone (vitamin K2)-deficient mutants of Bacillus subtilis, whose growth requirement is satisfied by 1,4-dihydroxy-2-naphthoic acid but not by o-succinylbenzoic acid (OSB), have been analyzed for enzymatic defects. Complementation analysis of cell-free extracts of the mutants revealed that there are two groups, as already indicated by genetic analysis. The missing enzyme in each group was identified by complementation of the cell-free extracts with o-succinylbenzoyl-coenzyme A (CoA) synthetase and dihydroxynaphthoate synthase extracted from Mycobacterium phlei. Mutants found to lack dihydroxynaphthoate synthase, and which therefore complement with dihydroxynaphthoate synthase of M. phlei, were designated as menB; those lacking o-succinylbenzoyl-CoA synthetase, and therefore complementing with o-succinylbenzoyl-CoA synthetase, were designated as menE. The menB mutants RB413 (men-325) and RB415 (men-329), when incubated with [2,3-14C2]OSB, produced only the spirodilactone form of OSB in a reaction that was CoA and adenosine 5'-triphosphate dependent.
D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D002087:Butyrates; D003066:Coenzyme A Ligases / Q000378:metabolism*; D005816:Genetic Complementation Test; D006836:Hydro-Lyases / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000096:biosynthesis; D009154:Mutation; D009284:Naphthols / Q000096:biosynthesis; D010654:Phenylbutyrates / Q000378:metabolism; D013141:Spiro Compounds / Q000096:biosynthesis; D013389:Succinate-CoA Ligases / Q000378:metabolism*
C017111:4-(2'-carboxyphenyl)-4,4-dihydroxybutyrate spirodilactone; D002087:Butyrates; D006885:Hydroxybutyrates; D009284:Naphthols; D010654:Phenylbutyrates; D013141:Spiro Compounds; C024023:1,4-dihydroxy-2-naphthoic acid; D006836:Hydro-Lyases; C021853:naphthoate synthetase; D003066:Coenzyme A Ligases; D013389:Succinate-CoA Ligases; C022693:O-succinylbenzoate - CoA ligase