[Trials of in vitro propagation of the scrapie agent in mouse nerve cells].
We attempted to propagate the Scrapie agent in vitro in glia and neuroblastoma cells of Mice. Four out of seven assays of infection were positive, i.e. after several passages in vitro yielding at most a 10(9) fold final dilution of the original material, the extracts of each of the four cultures, when injected intracerebrally into CD1 Mice, produced a deadly disease displaying the clinical and pathological signs characteristic of Scrapie.
D000818:Animals; D051379:Mice; D008805:Mice, Inbred A; D009374:Neoplasms, Experimental; D009447:Neuroblastoma; D009457:Neuroglia; D011328:Prions / Q000502:physiology*; D014776:Virus Cultivation; D014779:Virus Replication
D011328:Prions
Essais de propagation in vitro de l'agent scrapie dans des cellules nerveuses de souris.