The participation of primary amino groups of succinate dehydrogenase in the formation of succinate-Q reductase.
(1) Purified succinate dehydrogenase contains about 49 mol of lysine residues per mol enzyme. Titration of succinate dehydrogenase with fluorescamine indicates that half the lysyl groups are located on the surface of the protein and the other half are buried inside. (2) The reconstitutive activity and the low Km ferricyanide reductase activity of succinate dehydrogenase decreased as the extent of alkylation of amino groups by fluorescamine increased. (3) The inhibitory effects of fluorescamine on both activities are parallel and are succinate concentration dependent. (4) Alkylation of the native succinate-Q reductase by fluorescamine does not affect the enzymatic activity or alter the enzyme kinetic parameters. This indicates that the inhibitory effect of fluorescamine on succinate dehydrogenase is due to the modification of a specific amino group(s) on succinate dehydrogenase which is essential in the interaction with QPs to form succinate-Q reductase. The participation of an ionic group in the formation of succinate-Q reductase supports the idea of the involvement of ionic interaction between succinate dehydrogenase and QPs.
D000478:Alkylation; D002352:Carrier Proteins / Q000378:metabolism; D042963:Electron Transport Complex II; D005450:Fluorescamine / Q000494:pharmacology; D008239:Lysine / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D011487:Protein Conformation / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013386:Succinates / Q000494:pharmacology; D019802:Succinic Acid; D014451:Ubiquinone / Q000378:metabolism
D002352:Carrier Proteins; D009097:Multienzyme Complexes; D013386:Succinates; C027208:ubiquinone-binding proteins; D014451:Ubiquinone; D005450:Fluorescamine; D019802:Succinic Acid; D010088:Oxidoreductases; D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase; D008239:Lysine