Modified Dupont aca calcium method for hemolyzed specimens.
The presence of hemoglobin in serum or plasma causes an increase in the apparent calcium concentration when this determination is performed on the DuPont Automatic Clinical Analyzer (aca). A simple pack modification is described using ethylene glycol-bis-(2-aminoethylether)-tetracetic acid (EGTA) which permits estimation of the positive interference due to hemoglobin color. A correction factor is applied to enable the accurate measurement of calcium in hemolyzed samples.
D001774:Blood Chemical Analysis / Q000379:methods*; D002118:Calcium / Q000097:blood*; D004533:Egtazic Acid; D006454:Hemoglobins; D006461:Hemolysis; D006801:Humans
D006454:Hemoglobins; D004533:Egtazic Acid; D002118:Calcium