Tyrosinase immobilized within artificial cells for detoxification in liver failure: I. preparation and in vitro studies.
The in vitro removal of tyrosine by means of perfusion over immobilized tyrosinase within artificial cells was studied. The assayed activity of the microencapsulated tyrosinase was about half the value of the same amount of enzyme in free solution. Further studies were conducted to determine the stability, kinetics, and clearance of this system. The results obtained suggest that immobilized tyrosinase within artificial cells is efficient in removing tyrosine in vitro and further study in an animal model is feasible.
D001187:Artificial Organs; D001769:Blood; D004156:Catechol Oxidase / Q000627:therapeutic use*; D008099:Liver; D008107:Liver Diseases / Q000097:blood / Q000628:therapy; D014442:Monophenol Monooxygenase / Q000627:therapeutic use*; D013997:Time Factors; D014443:Tyrosine / Q000097:blood; D014462:Ultrafiltration / Q000379:methods*
D014443:Tyrosine; D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase