[Properties of the membrane-bound acetylcholine receptor from cow brain nucleus caudatus].
A new procedure for isolation of the membrane-bound acetylcholine receptor from cow brain N. caudatus is proposed. The procedure described includes tissue disruption by homogenization and ultrasonication, isolation of a crude membrane fraction and subsequent washing of the membranes with 1 M NaCl and 0,6 M KCl. The receptor yield is 40 nmoles per 1 g of tissue. The receptor is of a muscarine type. The K alpha values for the complexes with acetylcholine (3,8.10(-5) M), D,L-muscarine (3,7.10(-5) M), pilocarpine (3,7.10(-5) M), methylfurmethide (3,7.10(-5) M), atropine (2,5.10(-8) M and 1,2.10(-9) M) and platyphyllin (2,3.10(-7) M) were determined. The atropine binding within the pH range of 5 to 8 is weakly dependent on concentrations of Ca2+, Mg2+ and EDTA and EGTA sodium salts as well as on pH. Atropine binding is inhibited by high concentrations of NaCl. It is concluded that N. caudatus from cow brain is a convenient source for isolation of the muscarine acetylcholine receptor.
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D002421:Caudate Nucleus / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D011950:Receptors, Cholinergic / Q000302:isolation & purification / Q000378:metabolism*; D013329:Structure-Activity Relationship
D011950:Receptors, Cholinergic; D004533:Egtazic Acid; D004492:Edetic Acid; D008274:Magnesium; D000109:Acetylcholine; D002118:Calcium
Svoĩstva membranosviazannogo atsetilkholinovogo retseptora iz Nucleus caudatus mozga korovy.