Identification of carbonic anhydrase in chick growth-plate cartilage.
Carbonic anhydrase has been localized by immunocytochemistry in the cells and territorial matrix of the hypertrophic and calcifying zones of chick growth-plate cartilage. Adjacent epiphyseal and articular cartilage were not stained. By biochemical assay the activity levels were 61.3, 1.8, 34.7, and 703.3 units/g wet weight for growth plate, epiphyseal/articular cartilage, spongiosa, and blood, respectively. The role of the enzyme in growth plate is discussed.
D000818:Animals; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002356:Cartilage / Q000201:enzymology*; D002358:Cartilage, Articular / Q000201:enzymology; D002645:Chickens; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques
D002256:Carbonic Anhydrases