Solid phase enzyme immunoassay of tobramycin.
We have developed a competitive enzyme immunoassay (EIA) using co-polymer of styrene and maleate as the solid phase and glutaraldehyde for binding of the antibody to the solid phase. This assay showed a striking efficacy in its use for the assay of tobramycin (TOB). Furthermore, the pharmacokinetics of TOB were studied in six healthy male volunteers after the administration of 60- or 90-mg doses intramuscularly.
D000328:Adult; D000900:Anti-Bacterial Agents / Q000097:blood*; D005976:Glutaral / Q000494:pharmacology; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D014031:Tobramycin / Q000097:blood*
D000900:Anti-Bacterial Agents; D005976:Glutaral; D014031:Tobramycin