Monoclonal antibody OKT-9 recognizes the receptor for transferrin on human acute lymphocytic leukemia cells.
The monoclonal antibody OKT-9 recognizes a surface protein of human lymphocytes that consists of a disulfide bonded homodimer of m.w. 200,000 intact and 95,000 reduced. A similar protein is precipitated by transferrin-agarose, but not by agarose alone. Peptide mapping by limited proteolysis shows that the proteins precipitated by OKT-9 antibodies and transferrin-agarose are homologous. It is concluded that OKT-9 antibodies recognize the transferrin receptor. Expression of receptors for transferrin may be useful as a marker for activated or dividing cells.
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007945:Leukemia, Lymphoid / Q000276:immunology*; D051379:Mice; D008970:Molecular Weight; D011971:Receptors, Immunologic; D012756:Sheep; D014168:Transferrin / Q000378:metabolism*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D011971:Receptors, Immunologic; D014168:Transferrin