The interaction of human plasma fibronectin with a subunit of the first component of complement, C1q.
Fibronectin is a normal plasma protein that enhances reticuloendothelial system functioning, and may participate in immune complex clearance. The interaction of 125I-fibronectin with human C1 and C1q in vitro was investigated by employing a highly reproducible solid-phase binding assay in microtiter wells. We demonstrated that although fibronectin does not bind to antigen-antibody complex (BSA-anti-BSA) or immune complexes containing C1, a 20-fold increase in binding was obtained when the complexes contained C1q alone. In the absence of antigen-antibody complexes, fibronectin binds to the C1q fixed to the wells in a dose-response fashion but not to intact C1. C1q in the fluid phase inhibits 85% of the fibronectin binding to immobilized C1q. The amount of fibronectin bound by immobilized C1q or gelatin is approximately equal. The binding of fibronectin to C1q could be inhibited by the restoration of C1r + C1s to the C1 macromolecular complex before the addition of fibronectin. The inhibition was dependent on the concentration of C1r + C1s and achieved a maximum of 70% at 100 micrograms/ml. This inhibition could be reversed by the removal of C1r and C1s subunits with EDTA or C1 inhibitor. Digestion of C1q with pepsin resulted in an 85% loss of fibronectin binding. It therefore appears that at least one site of fibronectin binding to C1q is in the globular portion of this complement component.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism; D002417:Cattle; D003166:Complement Activating Enzymes / Q000378:metabolism*; D003167:Complement Activation; D003172:Complement C1 / Q000378:metabolism; D015922:Complement C1q; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D010434:Pepsin A / Q000494:pharmacology; D011817:Rabbits; D011951:Receptors, Complement; D012710:Serum Albumin, Bovine / Q000276:immunology
D000936:Antigen-Antibody Complex; D003172:Complement C1; D005353:Fibronectins; D011951:Receptors, Complement; D012710:Serum Albumin, Bovine; D015922:Complement C1q; D003166:Complement Activating Enzymes; D010434:Pepsin A