title: Prediction of the three-dimensional structure of the leader sequence of pre-kappa light chain, a hexadecapeptide.
abstract: The three-dimensional structure of the signal sequence for murine kappa light chain has been calculated by using conformational energy calculations. These calculations, based on tested and reliable potential energy functions, employ a novel global search technique to identify the lowest energy structures for the hexadecapeptide signal sequence, Glu-Thr-Asp-Thr-(Leu3-Trp-Val)2-Pro-Gly.l It has been found that the core hydrophobic sequence, Leu3-Trp-Val-Leu3, adopts an alpha-helical conformation that is terminated by chain reversal conformations for the four residues, Trp-Val-Pro-Gly. The amino-terminal four residues adopt low energy conformations that are fully compatible with the succeeding alpha-helix. The immediately neighboring sequence., Asp-Thr, exists in a single lowest energy double-equatorial conformation, whereas the first two residues, Glu-Thr, can adopt a variety of low energy conformations. The calculations arrive at a highly structured and specific model for the conformation of a leader sequence, compatible with recent experimental data.
mesh_terms: D000595:Amino Acid Sequence; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D008958:Models, Molecular; D010455:Peptides; D011487:Protein Conformation; D011498:Protein Precursors; D021382:Protein Sorting Signals; D013329:Structure-Activity Relationship; D013816:Thermodynamics
chemical_list: D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D010455:Peptides; D011498:Protein Precursors; D021382:Protein Sorting Signals