[In vitro binding of 70S ribosomes to membrane structures of Escherichia coli].
It was found that the maximal disattachment of the ribosomes from the membrane structures is observed upon their treatment with 10 mM tris-HCl buffer, pH 7.5, containing 250 mM sucrose, 750 mM KCl, 5 mM magnesium acetate and 1 mM EDTA or puromycin. The most effective attachment of ribosomes to the membrane occurs in 10 mM tris-HCl buffer, pH 7.5, containing 5% sucrose and Mg2+. The increase of Mg2+ concentration in the medium from 0.5 mM up to 1 mM results in a 2-fold increase of the ribosomes bound to the membranes. The concentration of the ribosomal material involved in the reaction is very essential for ribosome binding to the membranes. The amount of ribosomes bound to the membranes increases proportionally to the increase of the ribosome concentration in the reaction mixture.
D002458:Cell Fractionation; D002462:Cell Membrane / Q000648:ultrastructure; D004492:Edetic Acid; D004926:Escherichia coli / Q000648:ultrastructure*; D008274:Magnesium; D012270:Ribosomes / Q000648:ultrastructure*
D004492:Edetic Acid; D008274:Magnesium
Prisoedinenie 70S-ribosom k membrannym strukturam Escherichia coli in Vitro.