[Interaction of histones with DNA in chromatin. A new method of covalent binding of histones to DNA available for their localization on DNA].
A new method for covalent binding of histones to partially apurinized DNA was developed. Partial apurinization of DNA methylated within the composition of chromatin results in a formation of aldehyde groups interacting with the epsilon-amino groups of chromatin proteins lysine residues. The resulting Schiff's bases covalently and reversibly bind the protein molecules to DNA. This covalent binding is accompanied by a specific one-chain cleavage of DNA at the cross-linkage point in such a way that only the newly formed 5'-terminal fragment of DNA in bound to the protein. These cross-links can be stabilized via reduction of Schiff's bases by sodium borohydrate. Determination of the size of the bound DNA fragment allows to establish the localization of the cross-linkage point and the position of the protein molecule on DNA. The method of cross-linkage with a "zero length" allows to fix the immediate DNA--protein interactions and can be extensively used to study the protein--DNA interactions in cases when the epsilon-amino groups of protein lysine residues interact with DNA.
D000818:Animals; D001080:Apurinic Acid; D002417:Cattle; D002843:Chromatin / Q000032:analysis / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D007700:Kinetics; D008239:Lysine; D051379:Mice; D011485:Protein Binding; D012545:Schiff Bases
D002843:Chromatin; D006657:Histones; D012545:Schiff Bases; D001080:Apurinic Acid; D004247:DNA; D008239:Lysine
Vzaimodeistvie gistonov s DNK v khromatine. Novyi metod kovalentnogo sviazyvaniia gistonov s DNK, udobnyĭ dlia ikh lokalizatsii na DNK.