Structural and immunological studies of the Haemophilus influenzae type d capsular polysaccharide.
Employing a combination of chemical and spectroscopic techniques, the structure of the Haemophilus influenzae type d capsular polysaccharide was found to be leads to 4)-beta-D-GlcNAc-(1 leads to 3)-beta-D-ManNAcA-(1 leads to. L-Alanine, L-serine, and L-threonine, in the molar ratios of approximately 1.0:1.0:0.3, were linked to C-6 of the D-mannosyluronic residue as amides; the (serine + alanine + threonine) to ManNAcA ratio was approximately 0.95:1.0. Removal of the amino acids by mild hydrolysis with sodium hydroxide resulted in a material that was cross-reactive with the native, type d polymer. The base-treated, type d polysaccharide was not observed to cross-react with either the H. influenzae type e or Escherichia coli K7 capsular polysaccharide, both of which are structurally similar to type d.
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006193:Haemophilus influenzae / Q000276:immunology*; D005779:Immunodiffusion; D009682:Magnetic Resonance Spectroscopy; D011135:Polysaccharides, Bacterial / Q000276:immunology; D011233:Precipitin Tests
D011135:Polysaccharides, Bacterial