Initiation of protein synthesis in cell-free extracts of Tetrahymena pyriformis.
Saturating concentrations of the initiation-specific inhibitors poly(I) and T-2 toxin inhibit protein synthesis by over 35% and cause ribosome 'run-off' from the polyribosomes. The elongation-specific inhibitor cycloheximide totally prevents protein synthesis and 'freezes' the ribosomes in the pattern of unincubated controls. These results prove that our Tetrahymena extracts are capable of protein-synthesis initiation, a conclusion which is confirmed by a 30% inhibition of synthesis by the mRNA cap analogue, 7-methylguanosine 5'-monophosphate.
D000818:Animals; D002474:Cell-Free System / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects*; D011069:Poly I / Q000494:pharmacology; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D013605:T-2 Toxin / Q000494:pharmacology; D013769:Tetrahymena pyriformis / Q000187:drug effects / Q000378:metabolism*
D011069:Poly I; D003513:Cycloheximide; D013605:T-2 Toxin