Inhibition of the plasminogen activator urokinase by alpha-tocopherol.
alpha-Tocopherol (vitamin E) inhibited the activity of urokinase on fibrin plates and on the amidolytic substrate S-2444 in a concentration- and time-dependent manner. Inhibition was not removed by dialysis. In the presence of dilutions of plasma, protein-containing fractions of gel-filtered plasma, or bovine serum albumin, the inhibition of urokinase by alpha-tocopherol was abolished. Urokinase activity was not inhibited by phytol, menadione or hydroquinone.
D000818:Animals; D002417:Cattle; D005342:Fibrinolysis / Q000187:drug effects; D006801:Humans; D006873:Hydroquinones / Q000494:pharmacology; D009842:Oligopeptides / Q000378:metabolism; D010836:Phytol / Q000494:pharmacology; D010949:Plasma / Q000378:metabolism; D010960:Plasminogen Activators / Q000037:antagonists & inhibitors*; D015849:Plasminogen Inactivators; D011480:Protease Inhibitors; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D014568:Urokinase-Type Plasminogen Activator / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*; D014812:Vitamin K / Q000494:pharmacology
D006873:Hydroquinones; D009842:Oligopeptides; D015849:Plasminogen Inactivators; D011480:Protease Inhibitors; D014812:Vitamin K; D014810:Vitamin E; D010836:Phytol; D012710:Serum Albumin, Bovine; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator