Alteration of the action potential of tissue cultured neuronal cells by growth in the presence of a polyunsaturated fatty acid.
The effects of alterations in membrane phospholipid fatty acid composition on the excitability of neuroblastoma X glioma hybrid cells, clone NG108-15, were examined using intracellular recording techniques. Cells were grown in the presence of arachidonate (20:4) added to the culture medium as a complex with bovine serum albumin. Exposure of the cells to 20:4 for 3-21 days produced a 40% decrease in the maximum rate of rise of the action potential (dV/dt) with a small change in its amplitude. The resting membrane potential and passive properties of the cells were unaffected. An effect of 20:4 was not observed until 24 hr after treatment and increased over the next 2 days. The phospholipid content of 20:4 and its metabolite 22:4 increased from 6.9% to 25.3% of total fatty acids during approximately the same time span. It is concluded that the action potential dV/dt can be altered by changes in membrane lipid composition.
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology*; D002460:Cell Line; D002999:Clone Cells; D005910:Glioma / Q000503:physiopathology*; D006822:Hybrid Cells / Q000187:drug effects / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D009447:Neuroblastoma / Q000503:physiopathology; D009474:Neurons / Q000502:physiology*; D051381:Rats
D001095:Arachidonic Acids; D016718:Arachidonic Acid