Enzyme-immunoassay: a powerful analytical tool.
This paper reviews various aspects of enzyme-immunoassay (EIA). Firstly it summarizes the principles of tests in which use is made of labelled antigen or antibody. Since these constitute essential reagents in EIA they are discussed next. Many assay principles call for a bound/free separation. The various methods to accomplish this are therefore briefly discussed. Very important are the characteristics of EIA: specificity, sensitivity, precision and practicability, the latter including reagent stability, performance requirements, assay times and automation potential. Finally, fields of application are listed and some more recent developments are mentioned.
D000371:Agglutination; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D006063:Chorionic Gonadotropin; D003168:Complement Fixation Tests; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000378:metabolism; D007106:Immune Sera / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D008291:Malate Dehydrogenase / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism; D010928:Placental Lactogen; D011817:Rabbits; D001616:beta-Galactosidase / Q000378:metabolism
D006063:Chorionic Gonadotropin; D007106:Immune Sera; D010928:Placental Lactogen; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase; D009113:Muramidase; D001616:beta-Galactosidase