Reversed-phase ion-pair partion chromatography of biogenic catecholamines and their alpha-methyl homologues with tributylphosphate as stationary phase.
Ion-pair partition chromatography is applied to the separation of the biogenic catecholamines and their alpha-methyl homologues. A useful selectivity has been obtained using an adduct-forming organic stationary phase (tributylphosphate). The retention of the compounds can be regulated easily by means of the concentration of the counter-ion (the perchlorate ion) in the mobile phase. The selectivity for separation of amines from amino acids can be influenced by changing the pH of the aqueous phase. The phase system shows a good long-term stability and reproducibility with respect to the capacity ratios and the efficiency.
D008735:5-Methoxytryptamine / Q000032:analysis; D002395:Catecholamines / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D003846:Deoxyepinephrine / Q000032:analysis; D004295:Dihydroxyphenylalanine / Q000032:analysis; D004298:Dopamine / Q000032:analysis; D004837:Epinephrine / Q000032:analysis; D008676:Metanephrine / Q000032:analysis; D008750:Methyldopa / Q000032:analysis; D009638:Norepinephrine / Q000032:analysis
D002395:Catecholamines; D008735:5-Methoxytryptamine; D008676:Metanephrine; D008750:Methyldopa; D004295:Dihydroxyphenylalanine; D003846:Deoxyepinephrine; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine