Proposals on the control of VERO cells used to prepare inactivated poliomyelitis vaccine.
The purpose of this study is to know if it is possible to prepare inactivated poliomyelitis vaccines on Vero cell culture. In the cloned cell line used to prepare the vaccines, we have looked for an eventual toxic effect by different system tests. The cells between the 148e and 151e passage used to prepare the vaccines were inoculated into: - suckling mice, rabbits and guinea pigs - embryonated eggs. The supernatant fluid of the cells was inoculated into: - Vero cells - Primary rabbit kidney cells - Primary monkey kidney cells. We have also looked for an eventual tumorigenic effect on immunodepressed mice with an antithymocytic serum. The results of the assays are presented and discussed.
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured / Q000473:pathology*; D002642:Chick Embryo; D006168:Guinea Pigs; D000882:Haplorhini; D007668:Kidney; D051379:Mice; D011054:Poliovirus Vaccine, Inactivated / Q000592:standards*; D011817:Rabbits; D014613:Vaccines, Attenuated
D011054:Poliovirus Vaccine, Inactivated; D014613:Vaccines, Attenuated