title: Null effect of dietary Aroclor 1254 on hepatocellular carcinoma incidence in rainbow trout (Salmo gairdneri) exposed to aflatoxin B1 as embryos.
abstract: Four lots of 200 rainbow trout embryos were exposed to 200 ml solutions of aqueous 0.5 ppm aflatoxin B1 (AFB1) for 1 hr, and 4 lots of 200 rainbow trout embryos were sham-treated for 1 hr. After hatching and swimup, 100 fry from each group were selected for a feeding trial. Two groups of AFB1-treated and 2 groups of sham-treated fry were fed a control diet, while corresponding groups of AFB1-treated and sham-treated fry were fed the control diet plus 100 ppm Aroclor 1254 for 1 year. Samples were taken at 9 and 12 mon to monitor tumor incidence, Aroclor 1254 accumulation and histopathology. At the end of 12 mon, the liver cancer incidences in the combined AFB1-treated, control diet groups and the AFB1-treated, Aroclor 1254 diet groups were essentially the same. 65.8% and 63.9%, respectively. One trout in the sham-control, Aroclor 1254 diet group had a single liver cancer, but the sham-control, control diet group had none. The Aroclor 1254 fed groups had significantly higher liver to body weight ratios at 12 noon but not at 9 mon. Levels of accumulated Aroclor 1254 were similar to those reported previously, approximately 75 ppm on a whole fish basis at 12 mon. Glycogen depletion of hepatocytes and splenic hyperemia were the only tissue changes induced by Aroclor 1254.
mesh_terms: D016604:Aflatoxin B1; D000348:Aflatoxins / Q000633:toxicity*; D000818:Animals; D001140:Aroclors / Q000633:toxicity*; D020111:Chlorodiphenyl (54% Chlorine); D004032:Diet; D004347:Drug Interactions; D050356:Lipid Metabolism; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced*; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D012487:Salmonidae / Q000502:physiology*; D014337:Trout / Q000196:embryology / Q000502:physiology*
chemical_list: D000348:Aflatoxins; D001140:Aroclors; D020111:Chlorodiphenyl (54% Chlorine); D016604:Aflatoxin B1; D011078:Polychlorinated Biphenyls