Levels of enzymes involved in the synthesis of acetate from CO2 in Clostridium thermoautotrophicum.
The acetogenic bacterium Clostridium thermoautotrophicum, grown on methanol, glucose, or CO2-H2, contained high levels of corrinoids, formate dehydrogenase, tetrahydrofolate enzymes, carbon monoxide dehydrogenase, and hydrogenase. Cell-free extracts catalyzed pyruvate-dependent formation of acetate from methyltetrahydrofolate. These results suggest that C. thermoautotrophicum synthesizes acetate from CO2 via a formate-tetrahydrofolate-corrinoid pathway.
D000085:Acetates / Q000378:metabolism*; D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D003013:Clostridium / Q000201:enzymology*; D005560:Formate Dehydrogenases / Q000378:metabolism; D006864:Hydrogenase; D007700:Kinetics; D009097:Multienzyme Complexes; D010088:Oxidoreductases / Q000378:metabolism*
D000085:Acetates; D009097:Multienzyme Complexes; D002245:Carbon Dioxide; D010088:Oxidoreductases; D006864:Hydrogenase; D005560:Formate Dehydrogenases; D000445:Aldehyde Oxidoreductases; C019823:carbon monoxide dehydrogenase