title: Modified Dupont aca calcium method for hemolyzed specimens.
abstract: The presence of hemoglobin in serum or plasma causes an increase in the apparent calcium concentration when this determination is performed on the DuPont Automatic Clinical Analyzer (aca). A simple pack modification is described using ethylene glycol-bis-(2-aminoethylether)-tetracetic acid (EGTA) which permits estimation of the positive interference due to hemoglobin color. A correction factor is applied to enable the accurate measurement of calcium in hemolyzed samples.
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D002118:Calcium / Q000097:blood*; D004533:Egtazic Acid; D006454:Hemoglobins; D006461:Hemolysis; D006801:Humans
chemical_list: D006454:Hemoglobins; D004533:Egtazic Acid; D002118:Calcium