The deoxyglucose method for insects: towards electron microscopical resolution.
The problems of extending the deoxyglucose method for insects to electron microscopical resolution are discussed. It is calculated that several curies of 3H-deoxyglucose per kilogram body weight may be needed for identification of activity in ultra-thin sections. Oral application may be superior to injection. Incorporation of experimental and control stimulus in the same animal seems appropriate. Freeze-drying of Drosophila results in structural preservation sufficient for cell identification in the cervical connective. Autoradiography of ultra-thin sections has not yet been carried out.
D000818:Animals; D001345:Autoradiography; D003837:Deoxy Sugars; D003847:Deoxyglucose; D004331:Drosophila melanogaster / Q000502:physiology*; D005612:Freeze Drying; D008854:Microscopy, Electron; D008867:Microtomy / Q000379:methods; D014795:Visual Pathways / Q000502:physiology*; D014867:Water
D003837:Deoxy Sugars; D014867:Water; D003847:Deoxyglucose