Inhibition of platelet lipoxygenase by toluene-3,4-dithiol and other ferric iron chelators.
Arachidonate lipoxygenase, obtained from the soluble, cytoplasmic fraction of sonicated human platelets was strongly inhibited by toluene-3,4-dithiol and dithizone, agents which form strong complexes with ferric but not ferrous ions. Weak inhibition was noted with the less specific iron chelator ortho-phenanthroline and with potassium cyanide. There was not inhibition with the ferrous iron chelating agents 2,2' dipyridyl nor with bathophenanthroline or EDTA. These findings suggest that platelet lipoxygenase activity depends on ferric iron.
D001091:Arachidonate Lipoxygenases; D001095:Arachidonic Acids / Q000378:metabolism; D001391:Azo Compounds / Q000494:pharmacology*; D001792:Blood Platelets / Q000201:enzymology*; D002614:Chelating Agents / Q000494:pharmacology*; D004230:Dithizone / Q000494:pharmacology*; D006801:Humans; D016859:Lipoxygenase Inhibitors; D010618:Phenanthrolines / Q000494:pharmacology; D011190:Potassium Cyanide / Q000494:pharmacology; D014050:Toluene / Q000031:analogs & derivatives* / Q000494:pharmacology
D001095:Arachidonic Acids; D001391:Azo Compounds; D002614:Chelating Agents; D016859:Lipoxygenase Inhibitors; D010618:Phenanthrolines; D014050:Toluene; D004230:Dithizone; D001091:Arachidonate Lipoxygenases; D011190:Potassium Cyanide; C004848:dithiol