The use of glutaraldehyde and tannic acid to preserve reconstituted collagen for electron microscopy.
The effects of glutaraldehyde and tannic acid on the axial periodicity of collagen have measured. Both fixatives produce axial shrinkage of the collagen but whereas glutaraldehyde produces 7% shrinkage, tannic acid produces only 2% shrinkage. The technique of carbon/platinum shadowing was used to estimate the extent to which the collagen fibrils flatten down when they are dried onto grids for electron microscopy without prior embedding and sectioning. The influence of fixation was studied and it was found that minimum distortion occurred when both tannic acid and glutaraldehyde were used to preserve the protein structure.
D000447:Aldehydes; D000818:Animals; D002417:Cattle; D003094:Collagen / Q000378:metabolism*; D005404:Fixatives; D005976:Glutaral; D047348:Hydrolyzable Tannins; D008854:Microscopy, Electron; D013634:Tannins
D000447:Aldehydes; D005404:Fixatives; D047348:Hydrolyzable Tannins; D013634:Tannins; D003094:Collagen; D005976:Glutaral