title: VH families in the antibody response to p-azophenylarsonate: correlation between serology and amino acid sequence.
abstract: Anti-arsonate hybridoma antibodies of A/J mice have been grouped into 3 VH families by amino acid sequence analysis. One of these, the 91A3 family, which includes all CRI-positive molecules and some CRI-negative molecules, has been serologically defined by the presence of a VH marker (91A3 marker). Although the 91A3 family of antibodies is present at low levels in preimmune A/J serum and represents a substantial component of the A/J response to p-azophenylarsonate, the 91A3 family has not been detected serologically in the anti-Ar response of any other inbred strain tested. Analysis of sera from AXC Igh recombinant mice maps the 91A3 marker to the left of Igh-Dex, consistent with the location of the VH genes controlling the arsonate CRI. These studies establish a serologic assay that detects all members of a family of anti-arsonate antibodies defined previously by primary sequence analysis. This assay makes it possible to monitor in its entirety the expression of the products of a single or closely related group of genes.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001391:Azo Compounds; D001666:Binding Sites, Antibody / Q000235:genetics*; D003429:Cross Reactions; D006825:Hybridomas / Q000145:classification / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000276:immunology; D008297:Male; D051379:Mice; D008805:Mice, Inbred A / Q000235:genetics*; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB; D011995:Recombination, Genetic; D013045:Species Specificity; D010132:p-Azobenzenearsonate / Q000235:genetics* / Q000276:immunology
chemical_list: D001391:Azo Compounds; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007135:Immunoglobulin Variable Region; D010132:p-Azobenzenearsonate