Degradation of chondroitin 4-sulfate by rat stomach exoglycosidases, sulfohydrolase and hyaluronidase-like enzymes.
Rat stomach enzymes degrade chondroitin 4-sulfate (C4-S) by cooperative enzyme action yielding monosaccharides, SO4 (=) and oligosaccharides. Evidence for this was obtained by analyzing the effects of adding inhibitors and rate-limiting enzyme to incubation mixtures. A size preference appears to exist, since oligosaccharides of DP4-10 liberated by a gastric hyaluronidase-like enzyme is a poor substrate for desulfation by the gastric enzyme complex.
D000818:Animals; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000378:metabolism*; D002810:Chondroitinases and Chondroitin Lyases / Q000378:metabolism*; D002811:Chondroitinsulfatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006821:Hyaluronoglucosaminidase / Q000378:metabolism*; D008297:Male; D051381:Rats; D013270:Stomach / Q000201:enzymology*; D013997:Time Factors
D002807:Chondroitin; D002809:Chondroitin Sulfates; D002811:Chondroitinsulfatases; D006821:Hyaluronoglucosaminidase; D002810:Chondroitinases and Chondroitin Lyases