Assay of alpha-difluoromethylornithine in body fluids and tissues by automatic amino-acid analysis.
A procedure is described for the measurement of DL-alpha-difluoromethylornithine (DFMO), a selective irreversible inhibitor of ornithine decarboxylase, in biological specimens. The drug is separated from other amino acids with a commerical amino-acid analyser and detected by formation of its alkylthio-isoindole derivative with o-phthalaldehyde. DFMO concentrations of 0.1 nmol can be determined in a sample volume of 100 microliter. The assay has been used to determine the half-life of DFMO in serum of several species and the relationships between serum and tissue concentrations.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001322:Autoanalysis / Q000379:methods; D004285:Dogs; D000518:Eflornithine; D006207:Half-Life; D000882:Haplorhini; D006801:Humans; D009952:Ornithine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D011817:Rabbits; D051381:Rats
D000596:Amino Acids; D009952:Ornithine; D000518:Eflornithine