[Accuracy of HDL-cholesterol and HDL-apolipoprotein A-I determinations after phosphotungstate/MgCl2-precipitation of apolipoprotein B-containing lipoproteins (author's transl)].
With optimal concentrations of reagents a complete precipitation of apolipoprotein B-containing lipoproteins and only a small coprecipitation of HDL can be achieved using the phosphotungstate/MgCl2 precipitation method. The accuracy of the precipitation is not influenced by triglyceride concentrations less than 4.0 mmol/l. Similarly, in most hypertriglyceridaemic sera (greater than 4.0 mmol/l) a complete precipitation of apolipoprotein B-containing lipoproteins and a complete recovery of HDL can be observed. In addition to the HDL cholesterol determination, apolipoprotein A-I was determined by kinetic nephelometry. In hypertriglyceridaemic sera (greater than 4.56 mmol/l) precipitation of apolipoprotein B-containing lipoproteins must be carried out prior to the nephelometric determination of apolipoprotein A-I.
D000375:Aging; D016632:Apolipoprotein A-I; D001053:Apolipoproteins / Q000097:blood*; D001055:Apolipoproteins B; D002784:Cholesterol / Q000097:blood*; D008076:Cholesterol, HDL; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008274:Magnesium; D015636:Magnesium Chloride; D010772:Phosphotungstic Acid; D014280:Triglycerides / Q000097:blood
D016632:Apolipoprotein A-I; D001053:Apolipoproteins; D001055:Apolipoproteins B; D008076:Cholesterol, HDL; D008075:Lipoproteins, HDL; D014280:Triglycerides; D015636:Magnesium Chloride; D010772:Phosphotungstic Acid; D002784:Cholesterol; D008274:Magnesium
Zur Richtigkeit der HDL-Cholesterin- und HDL-Apolipoprotein A-I-Bestimmung nach Phosphorwolframsäure/MgCl2-Präzipitation Apolipoprotein B-haltiger Lipoproteine.