title: Effects of halothane on the incorporation of [14C]-serine into phospholipid in the guinea-pig ileum.
abstract: 1 The effects of halothane on the incorporation of L-[3-14C]-serine into phospholipid were studied in the resting, innervated longitudinal muscle preparation of the guinea-pig ileum. 2 The anaesthetic, at clinical concentrations, caused a dose-dependent, partial inhibition of incorporation. The effect was rapid and reversible, and did not show characteristics of competitive inhibition. 3 The incorporation was reduced by a decrease in the Ca2+ concentration of the Krebs incubation buffer. Part of the activity persisted in the absence of added Ca2+ and this was most susceptible to inhibition by halothane. Sensitivity to external Ca2+ was not influenced at the halothane concentrations studied. 4 Evidence in support of the 14C-incorporation being due to L-[3-14C]-serine-phospholipid base-exchange activity included: (a) the rapid appearance of radioactivity in phosphatidylserine; (b) the kinetics of this incorporation in relation to that in phosphatidylethanolamine;  (c) its dependence on Ca2+, and (d) its sensitivity to 2,4-dinitrophenol and its sensitivity to temperature. 5 It is concluded that this preparation makes it possible for a membrane-bound lipid-dependent activity (L-serine-phospholipid base-exchange) to be studied in conditions of cellular integrity under which the normal functional effects of lipophilic drugs can be simultaneously tested. 6 A rapid gas-chromatographic assay for halothane from an aqueous medium is also described.
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002250:Carbon Radioisotopes; D004140:Dinitrophenols / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006168:Guinea Pigs; D006221:Halothane / Q000494:pharmacology*; D007082:Ileum / Q000378:metabolism; D008297:Male; D010743:Phospholipids / Q000096:biosynthesis*; D012694:Serine / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D004140:Dinitrophenols; D010743:Phospholipids; D012694:Serine; D002118:Calcium; D006221:Halothane