title: [Canine fibrinogen glycopeptides].
abstract: Canine fibrinogen was digested by a complex of proteases from Streptomyces griseus. The degradation products were purified by gel-filtration, DEAE-cellulose chromatography and electrophoresis, resulting in nine glycopeptides, eight of which contained aspartic acid and one--serine. The other amino acids were found only in trace amounts. The glycopeptides were shown to contain hexoamines, mannose, galactose and sialic acid. The oligosaccharide chains form a sequence of structurally similar variants. The individual microheterogeneity of canine fibrinogen with respect to carbohydrate chains was detected. A comparison of the carbohydrate composition of fibrinogen and glycopeptides suggests the presence of four carbohydrate chains in the protein molecule.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D004285:Dogs; D005340:Fibrinogen; D006020:Glycopeptides / Q000032:analysis; D010446:Peptide Fragments / Q000032:analysis; D010447:Peptide Hydrolases; D013305:Streptomyces griseus / Q000201:enzymology
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D006020:Glycopeptides; D010446:Peptide Fragments; D005340:Fibrinogen; D010447:Peptide Hydrolases
vernacular_title: Issledovanie glikopeptidov fibrinogena sobaki.