Binding of 99mTc plasminogen on fibrin.
Binding of plasminogen to fibrin was studied in vitro and in vivo using 99mTc Glu- and Lys-plasminogen. Binding of Glu-plasminogen on the clot was not observed in vitro, and in vivo in the dog. Conversely, the binding of Lys-plasminogen to fibrin displays a linear relationship to the concentration of Lys-plasminogen, up to doses exceeding equimolarity; thus suggesting the existence of several Lys-plasminogen binding sites on fibrin. Binding levels were identical, regardless of whether plasminogen was incubated in normal plasma or in plasma devoid of antiplasmin. In the dog, Lys-plasminogen bound specifically to the clot, however, clot sites could not be localized by scintigraphy in the dog or in man.
D000818:Animals; D001665:Binding Sites; D001769:Blood; D004285:Dogs; D005342:Fibrinolysis; D006801:Humans; D008239:Lysine / Q000378:metabolism; D010446:Peptide Fragments; D010958:Plasminogen / Q000172:deficiency / Q000378:metabolism*; D012965:Sodium Chloride; D013667:Technetium; D000979:alpha-2-Antiplasmin / Q000172:deficiency
D010446:Peptide Fragments; D000979:alpha-2-Antiplasmin; C018998:lysyl-plasminogen; D012965:Sodium Chloride; D013667:Technetium; D010958:Plasminogen; D008239:Lysine