[Parameters for constructing a test system for the isoenzymatic detection of meningococcal antibodies].
The optimum conditions for the detection of specific humoral antibodies in patients with meningococcal infection by the immunoenzymatic method have been determined: the sensitizing dose of the antigen is 20 microgram of protein per 1 ml for group A meningococcal antigen and 25 microgram of protein per 1 ml for group C meningococcal antigen, the pH of the buffer solutions is 5.0-6.0 for group A meningococcal antigen and 12.0 for group C meningococcal antigen, the time and temperature of incubation should be 18-20 hours at 14 degrees C. The diagnostic test system developed in this research is capable of detecting antibodies with the accuracy of several ng.
D000907:Antibodies, Bacterial / Q000032:analysis*; D000918:Antibody Specificity; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D008722:Methods; D009345:Neisseria meningitidis / Q000276:immunology*; D013696:Temperature; D013997:Time Factors
D000907:Antibodies, Bacterial
Parametry konstruirovaniia test-sistemy dlia vyiavleniia meningokokkovykh antitel immunofermentnym metodom.