[Electron microscopic study of the chromatin in hepatocyte nuclei in the 1st hours after a partial hepatectomy. IV. Increased sensitivity of active chromatin to EDTA exposure].
In the period of increasing the guinea-pig hepatocyte chromatin template activity, 2.5 hours after partial hepatectomy, an increased susceptibility of condensed chromatin to the bleaching action of EDTA in the Bernard reaction has been found. The condensed chromatin of the activated by partial hepatectomy guinea-pig hepatocytes, studied on ultrathin sections, is bleached under the action of EDTA more intensely compared to the chromatin of the control (non-activated) cells. Five hours after partial hepatectomy, when hepatocyte chromatin, according to its physico-chemical properties and functional activity, is the same as that of the control (non-operated) animals, its capacity of being bleached by EDTA also returns to the control level. In one nucleus studied on ultrathin sections the perinucleolar chromatin was found to be more sensitive to EDTA than the chromatin of other parts of the nucleus. It is suggested that the treatment with EDTA under given conditions can be used for revealing the functional state of chromatin on ultrathin sections.
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure*; D004492:Edetic Acid / Q000494:pharmacology*; D006168:Guinea Pigs; D006498:Hepatectomy; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D013997:Time Factors
D002843:Chromatin; D004492:Edetic Acid
Elektronno-mikroskopicheskoe izuchenie khromatina iader gepatotsitov v pervye chasy posle chastichnoĭ gepatéktomii. IV. Povyshennaia chuvstvitel'nost' aktivnogo khromatina k vozdeĭstviiu EDTA.