Effect of furosemide on collecting-duct hydrogen ion secretion in the rabbit.
The purpose of this study was to determine the effect of acute furosemide administration on urine acidification. U-B Pco2 was used to assess collecting-duct hydrogen ion secretion in rabbits infused with bicarbonate. Prior to furosemide administration, the U-B Pco2 was virtually zero, indicating very low or absent hydrogen ion secretion in the collecting duct. Intravenous furosemide infusion resulted in a significant increase in the U-B Pco2. This increase could not be attributed to changes in blood Pco2, plasma potassium concentration, or urine phosphate concentration and buffer capacity. Furthermore, the rise in U-B Pco2 was independent of any change in urine bicarbonate concentration. We conclude that furosemide leads to an augmentation of collecting-duct hydrogen ion secretion in the rabbit, perhaps by increasing sodium reabsorption in the collecting duct.
D000818:Animals; D001639:Bicarbonates / Q000652:urine; D002245:Carbon Dioxide / Q000097:blood; D002712:Chlorides / Q000652:urine; D005110:Extracellular Space; D005665:Furosemide / Q000008:administration & dosage*; D006863:Hydrogen-Ion Concentration; D007684:Kidney Tubules / Q000378:metabolism*; D007685:Kidney Tubules, Collecting / Q000378:metabolism*; D008297:Male; D010710:Phosphates / Q000097:blood; D011188:Potassium / Q000097:blood; D011817:Rabbits
D001639:Bicarbonates; D002712:Chlorides; D010710:Phosphates; D002245:Carbon Dioxide; D005665:Furosemide; D011188:Potassium