The extra-adrenal synthesis of epinephrine in rats. Possible involvement of dopamine sulfate.
Bilaterally adrenalectomized rats were administered [3H]L-dopa, [3H]DA, and [3H]DASO4. Both [3H]L-dopa and [3H]DASO4 were found to generate [3H]E in the urine and kidney tissues of adrenalectomized rats, whereas [3H]DA did not produce any detectable [3H]E. [3H]CAs in tissues and urine were analyzed by reverse-phase high-performance liquid chromatography. The identities of the [3H]CAs in the urine were further verified by a double-labeling radioenzymatic technique. The results demonstrated that in the rat E can by synthesized outside the adrenals and that DASO4 can serve as an intermediate in such a synthesis. [3H]DASO4 was also converted to [3H]DA and [3H]NE, indicating that it can be metabolized in vivo and could be the source of free DA in urine.
D000311:Adrenal Glands / Q000378:metabolism* / Q000502:physiology; D000315:Adrenalectomy; D000818:Animals; D002395:Catecholamines / Q000032:analysis / Q000652:urine; D004295:Dihydroxyphenylalanine / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism* / Q000031:analogs & derivatives; D004837:Epinephrine / Q000096:biosynthesis*; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D009206:Myocardium / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000032:analysis
D002395:Catecholamines; C022807:dopamine 4-O-sulfate; C007558:dopamine 3-O-sulfate; D004295:Dihydroxyphenylalanine; D004298:Dopamine; D004837:Epinephrine