Restrictions to the use of 111 indium-oxine as a radiolabel in lymphocyte migration studies.
Guinea pig T lymphocytes were labelled with various concentrations of 111In-oxine. The capacity of these cells to migrate into a skin inflammatory site was compared with that of 51Cr-labelled cells. The results show that even with low dosages of 111In-oxine which did not impair lymph node localization (1-10 microCi/10(8) cells/ml), migration into an inflammatory site was markedly reduced. Moreover, using this isotope, a significant contribution to the radioactivity recovered from an inflammatory site is made by the local accumulation of non-cell-bound label. These results stress the limited use of 111 In-oxine as a radiolabel in lymphocyte migration studies.
D000818:Animals; D002465:Cell Movement; D002470:Cell Survival; D002860:Chromium Radioisotopes; D004137:Dinitrochlorobenzene; D005260:Female; D006168:Guinea Pigs; D006912:Hydroxyquinolines; D007204:Indium; D008214:Lymphocytes / Q000378:metabolism*; D008222:Lymphokines / Q000096:biosynthesis; D015125:Oxyquinoline; D011868:Radioisotopes; D012882:Skin Tests
D002860:Chromium Radioisotopes; D004137:Dinitrochlorobenzene; D006912:Hydroxyquinolines; D008222:Lymphokines; D011868:Radioisotopes; D007204:Indium; D015125:Oxyquinoline