[Mechanism of inhibition by chlorgyline and deprenyl of tyramine deamination by mitochondrial monoamine oxidase of rat liver].
The inhibition by chlorgyline and deprenyl of deamination of tyramine, i. e. substrate of two forms of monoamine oxidase (MAO) A and B, by fragments of rat liver mitochondrial membrane and the effects of competitive reversible inhibitors of the MAO activity, e. g. 4-ethylpyridine, benzyl alcohol, O-benzyl-hydroxylamine and 2-oxyquinoline, on this process were studied. It was shown that all the inhibitors used sharply increase the inhibiting effect of chlorgyline on tyramine deamination, the degree of the stimulating effect being the same irrespective of whether the inhibitors are added to the samples before or after a 30-min preincubation of chlorgyline with the enzyme at 23 degrees, i. e. after the onset of irreversible inhibition. The stimulating effect is due to the independent action of two inhibitors on the two different sites of the MAO active center: chlorgyline--on the isoalloxazine ring of FAD, that of 4-ethylpyridine, benzyl alcohol, O-benzylhydroxylamine, 2-oxyquinoline, respectively, on the hydrophobic region involved in tyramine binding. In similar experiments with deprenyl all the competitive inhibitors used, when added to the samples after a 30-min incubation of the inhibitor with the enzyme at 23 degrees, remove the inhibiting effect of deprenyl on tyramine deamination. The decrease of the inhibiting effect of deprenyl is indicative of an existence of competitive interactions between deprenyl and the above-mentioned compounds and of the reversible inhibition by deprenyl of tyramine deamination under the given experimental conditions. The data obtained revealed the differences in the type and mechanism of action of chlorgyline and deprenyl on tyramine deamination and showed that these inhibitors act on different sites of the MAO active center, responsible for tyramine oxidation. Chlorgyline blocks primarily the "flavin moiety" of the MAO molecule, essential for the catalytic act, while the effect of deprenyl is directed to the hydrophobic part of the enzyme active center essential for the enzyme binding to tyramine. In this case the irreversible inhibiting effect is achieved at a slower rate and the reversibility of tyramine oxidation by deprenyl is maintained for a longer period of time than the chlorgyline inhibition of deamination of this amine.
D000818:Animals; D001667:Binding, Competitive; D003010:Clorgyline / Q000494:pharmacology*; D004789:Enzyme Activation; D007700:Kinetics; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D008996:Monoamine Oxidase Inhibitors; D010627:Phenethylamines / Q000494:pharmacology*; D011437:Propylamines / Q000494:pharmacology*; D011485:Protein Binding; D051381:Rats; D012642:Selegiline / Q000494:pharmacology*; D014439:Tyramine / Q000378:metabolism*
D008996:Monoamine Oxidase Inhibitors; D010627:Phenethylamines; D011437:Propylamines; D012642:Selegiline; D003010:Clorgyline; D014439:Tyramine
Omekhanizme tormozheniia khlorgilinom i deprenilom okislitel'nogo dezaminirovaniia tiramina mitokhondrial'noĭ monoaminoksidazoi pechenĭ krys.