title: Regulation of plasminogen activator secretion in mouse peritoneal macrophages. II. Inhibition by immunomodulators of bacterial origin.
abstract: Two classes of immunomodulators of bacterial origin, peptidoglycan derivatives and lipopolysaccharides, are able to block in vitro the production of plasminogen activator by elicited macrophages: the release of the enzyme into the medium is inhibited and the intracellular activity reduced. In the case of peptidoglycan derivatives, high molecular weight compounds like WSA (water-soluble adjuvant) are stronger inhibitors than small molecules like MPP (muramyl pentapeptide). MDP (muramyl dipeptide) gives partial inhibition only. WSA (at 100 micrograms/ml) completely inhibits plasminogen activator production; the inhibition is reversible and specific. LPS is active at low concentrations (25-100 ng/ml). At concentrations higher than 50-100 ng/ml the action of LPS becomes irreversible and less specific. Peptidoglycan-derived immunomodulators can inhibit plasminogen activator production in the presence of polymixin B or in the case of macrophages obtained from C3H/HeJ mice; LPS is inactive under such conditions.
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D001409:Bacillus cereus / Q000502:physiology; D018407:Bacterial Physiological Phenomena; D005260:Female; D006020:Glycopeptides / Q000494:pharmacology; D066298:In Vitro Techniques; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D009161:Mycobacterium / Q000502:physiology; D010960:Plasminogen Activators / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000494:pharmacology; D012477:Salmonella enteritidis / Q000502:physiology
chemical_list: D000276:Adjuvants, Immunologic; D006020:Glycopeptides; D011135:Polysaccharides, Bacterial; D010960:Plasminogen Activators