Buffer electrofocusing of Interleukin I.
Buffer electrofocusing, utilizing a mixture of low-molecular-weight buffer compounds to establish a stable linear pH gradient covering the range 3.5-6.0 on granulated gel media, has been employed in the purification of Interleukin I with good recovery of biological activity. The difficulties often experienced in the removal of commercial synthetic ampholytes after conventional preparative electrofocusing are avoided in this method.
D000665:Ampholyte Mixtures; D002021:Buffers; D004340:Drug Contamination; D006863:Hydrogen-Ion Concentration; D007375:Interleukin-1; D007525:Isoelectric Focusing / Q000379:methods*; D007526:Isoelectric Point; D011506:Proteins / Q000302:isolation & purification*
D000665:Ampholyte Mixtures; D002021:Buffers; D007375:Interleukin-1; D011506:Proteins