Affinity chromatography and immunosorption with acetylcholine receptor attached to nylon tubes.
Nylon-linked proteins were used for affinity trapping and chromatography. As representative examples purified acetylcholine receptor, alpha-cobratoxin and bovine serum albumin were coupled to the activated matrix to serve as biospecific ligands. In particular, acetylcholine receptor was coupled without significant loss of biochemical properties. The resulting affinity tubes bind receptor-specific ligands including immunoglobulins and thus can be used for affinity-chromatographic purposes and immunoassays.
D002846:Chromatography, Affinity / Q000379:methods*; D003039:Cobra Neurotoxin Proteins; D003432:Cross-Linking Reagents; D005022:Ethyldimethylaminopropyl Carbodiimide; D005976:Glutaral; D007136:Immunoglobulins / Q000302:isolation & purification; D007163:Immunosorbent Techniques; D008024:Ligands; D009757:Nylons; D011950:Receptors, Cholinergic; D012710:Serum Albumin, Bovine
D003039:Cobra Neurotoxin Proteins; D003432:Cross-Linking Reagents; D007136:Immunoglobulins; D008024:Ligands; D009757:Nylons; D011950:Receptors, Cholinergic; D012710:Serum Albumin, Bovine; C025154:alpha-cobratoxin; D005022:Ethyldimethylaminopropyl Carbodiimide; D005976:Glutaral