[The importance of the superoxide dismutases in histochemistry of dehydrogenases (author's transl)].
During the incubation of cryostat sections (various organs of rat) with aqueous media xanthine dehydrogenase and superoxide dismutase activities become soluble and detectable in the solution by biochemical methods. Using the tetrazolium technique the demonstration of xanthine dehydrogenase is successful only after separation from the superoxide dismutase by gel electrophoresis. The soluble flavine enzymee xanthine dehydrogenase is taken as an example to discuss the significance of superoxide dismutases in incubation media for the histochemical demonstration of dehydrogenating enzymes by tetrazolium methods.
D000818:Animals; D006651:Histocytochemistry; D007658:Ketone Oxidoreductases / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D013053:Spectrophotometry; D013482:Superoxide Dismutase / Q000302:isolation & purification / Q000378:metabolism*; D013778:Tetrazolium Salts; D014968:Xanthine Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*
D013778:Tetrazolium Salts; D013482:Superoxide Dismutase; D014968:Xanthine Dehydrogenase; D007658:Ketone Oxidoreductases
Zur Rolle der Superoxiddismutasen für den histochemischen Dehydrogenasenachweis.