An in vitro evaluation of two commercial blood culture media--supplemented peptone broth and supplemented tryptone soya broth.
Two commercial blood culture--supplemented peptone broth and supplemented tryptone soya broth--were compared by an in vitro method. The two media were inoculated with microorganisms suspended in fresh human blood in an attempt to mimic the clinical situation. The two media differed completely in their ability to support the growth of Neisseria meningitidis and Neisseria gonorrhoeae. Macroscopic evaluation of the blood culture medium during the incubation period was difficult when using supplemented tryptone soya broth in contrast to supplemented peptone broth. The former medium remained turbid whether microorganisms were growing or not. No other important differences between the two media were detected. Our findings indicate a need for both in vitro and in vivo studies to evaluate blood culture media and also a need for quality control of microbiological diagnostic devices before they are released for clinical use.
D001769:Blood; D003470:Culture Media; D005069:Evaluation Studies as Topic; D009344:Neisseria gonorrhoeae / Q000254:growth & development; D009345:Neisseria meningitidis / Q000254:growth & development; D010461:Peptones; D013025:Glycine max
D003470:Culture Media; D010461:Peptones