Adenine and adenosine metabolizing enzymes in cell-free extracts from Euglena gracilis.
1. Activities of the following enzymes involved in adenine and adenosine metabolism were found in cell-free extracts from Euglena gracilis: acid phosphatase (EC 3.1.3.2), 5'-methylthioadenosine phosphorylase (EC 2.4.2.-), adenine deaminase (EC 3.5.4.2), adenine phosphoribosyltransferase (EC 2.4.2.7) and adenosine kinase (EC 2.7.1.20). 2. The activities occurred both in heterotrophic and photoautotrophic cells and their levels did not change during light-induced chloroplast development. 3. Neither S-adenosylhomocysteinase (EC 3.3.1.1), 5'-methylthioadenosine nucleosidase (EC 3.2.2.9) and nucleoside phosphotransferase (EC 2.7.1.77) nor adenosine degrading enzymes: adenosine deaminase (EC 3.5.4.4), adenosine nucleosidase (EC 3.2.2.7), and purine-nucleoside (adenosine) phosphorylase (EC 2.4.2.1) were found in the Euglena extracts. 4. Comparison of the adenine and adenosine metabolism in Euglena and in other organisms is comprehensively presented. The metabolism in Euglena gracilis differs from that in higher animals and plants.
D000135:Acid Phosphatase / Q000378:metabolism; D000225:Adenine / Q000378:metabolism*; D000228:Adenine Phosphoribosyltransferase / Q000378:metabolism; D000241:Adenosine / Q000031:analogs & derivatives / Q000378:metabolism*; D000248:Adenosine Kinase / Q000378:metabolism; D000619:Aminohydrolases / Q000378:metabolism; D005056:Euglena gracilis / Q000201:enzymology*; D007700:Kinetics; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism; D013872:Thionucleosides / Q000378:metabolism
D013872:Thionucleosides; D011683:Purine-Nucleoside Phosphorylase; C021784:5'-methylthioadenosine phosphorylase; D000228:Adenine Phosphoribosyltransferase; D000248:Adenosine Kinase; D000135:Acid Phosphatase; D000619:Aminohydrolases; C018774:adenine deaminase; D000225:Adenine; D000241:Adenosine