[A simple fixation of antigens suited for the demonstration of autologous antibodies (author's transl)].
To determine autoantibodies against nuclear constituents, parietal cells, smooth muscles, mitochondria, microsomes of the thyreoidea and against myocard we use 4 micrometer cryostat tissue sections or monolayer cell preparations. After stepwise desiccation they are shortly heated to 100 degrees C. Antigenicity and histological structures are fully preserved for months, if these substrates were stored at -20 degrees C, provided that rehydration by even traces of moisture is carefully prevented.
D000818:Animals; D000941:Antigens; D001323:Autoantibodies / Q000032:analysis*; D003890:Desiccation; D005615:Freezing; D006358:Hot Temperature; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008861:Microsomes / Q000276:immunology; D008928:Mitochondria / Q000276:immunology; D009130:Muscle, Smooth / Q000276:immunology; D009206:Myocardium / Q000276:immunology; D051381:Rats; D013961:Thyroid Gland / Q000276:immunology
D000941:Antigens; D001323:Autoantibodies
Einfache Fixierung von Antigensubstraten für den Autoantikörpernachweis.