[Electrophoretic and immunological characteristics of non-histone proteins of human skin tumors. I) Methods].
The object of the research is to classify the nuclear proteins of the cellular nucleus of some types of human skin tumours (melanotic and amelanotic melanomas, squamous epitheliomas). Nuclear proteins were extracted from the nuclei with a saline buffer. Nuclear proteins were extracted from a melanoma step by step. After prolonged centrifugation to sediment the DNA and the large ribonucleoprotein complexes, the nuclear proteins were chromatographed on columns of Biorex 70+ to remove proteins were chromatographed on columns of Biorex 70+ to remove the histones. Nuclear proteins from a squamous epithelioma were injected into a rabbit to induce specific antibodies.
D002852:Chromatography, Ion Exchange / Q000379:methods; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D005779:Immunodiffusion; D009698:Nucleoproteins / Q000145:classification; D012878:Skin Neoplasms / Q000032:analysis*
D002868:Chromosomal Proteins, Non-Histone; D009698:Nucleoproteins
Caratteristiche elettroforetiche ed immunologiche delle proteine non-istoniche dei tumori cutanei umani. - I) Metodi.