Comparison of metabolic systems required to activate pro-mutagens/carcinogens in vitro for sister-chromatid exchange studies.
Irradiated Syrian hamster fetal cells (feeder layer) and rat-liver homogenate (S9 mix) were used to compare their capacity to metabolize 3 known promutagens/carcinogens; BaP, 3-MC and DMBA. DNA-damaging potential was determined by the induction of SCE in V79 target cells. The S9 mix (1/20th strength) was toxic to the target cells and reduced the mitotic index by half with an exposure time of 2.5 h. The feeder layer was not toxic to the target cells and, therefore, was included for the duration of the Expt. The test chemicals elicited a dose-response with both activating systems. At similar concentrations of the test chemicals, the cells grown on the feeder layer showed a greater number of SCEs as compared to those activated by the S9 mix.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000378:metabolism; D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism; D001711:Biotransformation; D002273:Carcinogens / Q000378:metabolism* / Q000494:pharmacology; D006224:Cricetinae; D003434:Crossing Over, Genetic / Q000187:drug effects*; D005333:Fetus / Q000378:metabolism*; D008647:Mesocricetus; D008748:Methylcholanthrene / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism*; D009153:Mutagens / Q000378:metabolism; D051381:Rats; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D001580:Benzopyrenes; D002273:Carcinogens; D009153:Mutagens; D008748:Methylcholanthrene; D015127:9,10-Dimethyl-1,2-benzanthracene