Alkaline DNA fragmentation in vivo: borderline or negative results obtained respectively with 7,12-dimethylbenz[a]anthracene and benzo[a]pyrene.
Using the in vivo DNA damage alkaline elution assay, a satisfactory correlation with carcinogenicity in the same target organ has been previously shown for a variety of chemical agents. This work was intended to enlarge the exploration of the predictivity of this test. Benzo[a]pyrene (BP) was found negative for damage to liver DNA of mice and rats, and 7,12-dimethylbenz[a]anthracene (DMBA) negative for damage to liver and bone marrow DNA of mice and slightly positive for damage to mammary gland DNA of young female rats. The results were found to be correlated with the extension of DNA arlyation in target organs in similar experimental conditions. From carcinogenicity data reported in the Survey of Compounds Which Have Been Tested for Carcinogenic Activity (vols. 1961-1973) BP and DMBA were both found to be essentially negative as liver carcinogens; however, DMBA was a potent carcinogen in inducing mammary tumors.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity*; D000818:Animals; D001551:Benz(a)Anthracenes / Q000633:toxicity*; D001580:Benzopyrenes / Q000633:toxicity*; D004247:DNA / Q000378:metabolism*; D004128:Dimethylnitrosamine / Q000633:toxicity; D005260:Female; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D009369:Neoplasms / Q000139:chemically induced; D051381:Rats
D001551:Benz(a)Anthracenes; D001580:Benzopyrenes; D015127:9,10-Dimethyl-1,2-benzanthracene; D004247:DNA; D004128:Dimethylnitrosamine