title: Sugar and amino acid transport from the rat uterine lumen: effects of estrogen and progesterone.
abstract: Sugar and amino acid transport and diffusion from the uterine lumen were evaluated in either castrated rats 4 or 24 h after iv injection of 17 beta-estradiol (0.1--10 microgram) or in estrogen-primed castrated rats 12 or 24 h after iv injection of progesterone (25--250 microgram). Uptake phenomena were evaluated by selectively exposing the uterine luminal surface to a Ringer's solution containing [U-14C]D-mannitol and [3H]3-O-methyl-D-glucose or [3H]alpha-aminoisobutyric acid for a 30-sec incubation. Diffusion (D-mannitol uptake) decreased significantly 4 h (but not 24 h) after estrogen and 12 and 24 h after progesterone treatment. [3H]3-O-methyl-D-glucose transport increased significantly both 4 h (120--380%) and 24 h (50--200%) after estrogen. Although [3H]3-O-methyl-D-glucose transport was not significantly changed from that in the vehicle-injected control 12 or 24 h after progesterone, comparison of the transport at 12 h (reduced) to that at 24 h (increased) revealed a significant difference in the responses to progesterone treatment at these two times. [3H]alpha-aminoisobutyric acid transport increased significantly 4 and 24 h after only a pharmacological dose of 17 beta-estradiol and was unchanged 24 h after progesterone administration. In summary, uterine luminal diffusion and transport phenomena are hormone sensitive, with estrogen exerting a more pronounced effect on transport than does progesterone. Thus, through modulation of luminal transport mechanisms, these hormones may regulate substrate levels and, hence, their availability to the developing conceptus during different reproductive states in the rat.
mesh_terms: D000621:Aminoisobutyric Acids / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002369:Castration; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D008353:Mannitol / Q000378:metabolism*; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000621:Aminoisobutyric Acids; D008757:Methylglucosides; D008759:Methylglycosides; D008353:Mannitol; D011374:Progesterone; D004958:Estradiol