Purification of Euglena gracilis chloroplast elongation factor Ts.
Previous studies have demonstrated that the activity of Euglena gracilis chloroplast elongation factor Ts is regulated by light and the gene for this factor is nuclear coded (Fox, L., Erion, J., Tarnowski, J., Spremulli, L., Brot, N., and Weissbach, H., J. Biol. Chem. (1980) 255, 6018-6019). Chloroplast elongation factor Ts has been purified to near homogeneity from E. gracilis extracts prepared from light-grown cells. It is composed of a single polypeptide chain of approximately 62,000 daltons. It interacts stoichiometrically with Escherichia coli elongation factor Tu to form an elongation factor Tu.elongation factor Ts complex.
D001426:Bacterial Proteins / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D005056:Euglena gracilis / Q000378:metabolism*; D008970:Molecular Weight; D010444:Peptide Elongation Factor Tu; D010445:Peptide Elongation Factors / Q000302:isolation & purification* / Q000378:metabolism; D013824:Thermus / Q000378:metabolism
D001426:Bacterial Proteins; D010445:Peptide Elongation Factors; C023512:elongation factor Ts; D010444:Peptide Elongation Factor Tu