Farnesyl pyrophosphate synthetase from Bacillus subtilis.
Farnesyl pyrophosphate synthetase was detected in extracts of Bacillus subtilis and partially purified by Sephadex G-100, hydroxylapatite, and DEAE-Sephadex chromatography. The enzyme catalyzed the exclusive formation of all-trans farnesyl pyrophosphate from isopentenyl pyrophosphate and either dimethylallyl or geranyl pyrophosphate. Mg2+ was essential for the catalytic activity and Mn2+ was less effective. The enzyme was slightly activated by sulfhydryl reagents. This enzyme was markedly stimulated by K+, NH4+, or detergents such as Triton X-100 and Tween 80, unlike the known farnesyl pyrophosphate synthetases from eucaryotes. The molecular weight of the enzyme was estimated by gel filtration to be 67,000. The Michaelis constants for dimethylallyl and geranyl pyrophosphate were 50 microM and 18 microM, respectively.
D000475:Alkenes / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology*; D001414:Bacitracin / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D004122:Dimethylallyltranstransferase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D005204:Farnesol / Q000031:analogs & derivatives / Q000096:biosynthesis; D045782:Hemiterpenes; D008274:Magnesium / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000378:metabolism; D011106:Polyisoprenyl Phosphates / Q000096:biosynthesis / Q000378:metabolism; D012717:Sesquiterpenes; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D014166:Transferases / Q000378:metabolism*
D000475:Alkenes; D003902:Detergents; D045782:Hemiterpenes; D009943:Organophosphorus Compounds; D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; D013439:Sulfhydryl Reagents; D001414:Bacitracin; C004809:isopentenyl pyrophosphate; D005204:Farnesol; C015234:geranyl pyrophosphate; C004808:farnesyl pyrophosphate; D014166:Transferases; D004122:Dimethylallyltranstransferase; D008274:Magnesium