Mutagenesis during transformation of Bacillus subtilis. II. An increase in chemically-induced mutations during competency.
During the development of competency in Bacillus subtilis there was an increased sensitivity to methyl methanesulfonate (MMS) treatments. The frequency of reverse mutation also increased among the MMS-revertible markers by a factor of 100 as compared to vegetative cultures. The frequency of 2-aminopurine(AP)-induced mutagenesis was the same in competent and noncompetent cultures. Studies with DNA-polymerase-deficient mutants showed a direct involvement of DNA polymerase I in promoting MMS and transformation-induced mutagenesis in competent cells.
D015075:2-Aminopurine / Q000494:pharmacology; D001412:Bacillus subtilis / Q000235:genetics*; D004256:DNA Polymerase I / Q000235:genetics*; D004269:DNA, Bacterial; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D005787:Gene Frequency; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D009154:Mutation / Q000187:drug effects; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D015075:2-Aminopurine; D008741:Methyl Methanesulfonate; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase