Permanent cell culture lines of rat erythroleukemia and growth inhibition by dexamethasone in vitro.
Four erythroleukemia cell lines were established in vitro from rats with primary leukemias induced by a set of intravenous injections of 7,8,12-trimethylbenz[a] anthracene (TMBA). These cell lines were grown in Dulbecco's medium with 20% inactivated fetal calf serum; they were characterized by specific consistent chromosome abnormalities, related to No. 2 chromosome, which were maintained in culture for more than 1 year. Addition of dexamethasone prevented the cell growth of 2 of the cell lines in this medium but failed to alter the growth rate of the other 2 cell lines. These findings are correlated with karyotype abnormalities involving chromosome No. 2.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000031:analogs & derivatives; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D007621:Karyotyping; D007942:Leukemia, Experimental / Q000139:chemically induced / Q000235:genetics / Q000503:physiopathology*; D051381:Rats
C029538:7,8,12-trimethylbenz(a)anthracene; D015127:9,10-Dimethyl-1,2-benzanthracene; D003907:Dexamethasone