title: hard tissue biochemistry: a comparison of fresh-frozen and formalin-fixed tissue samples.
abstract: Formalin fixation of hard tissues alters the levels of some, but not all, of the parameters routinely measured by bone researchers. Although mineral parameters (ash weight, particle size (beta 1/2 002) and Ca/PO4 ratio) and lipid parameters (total lipid content and composition, extractability, and Ca-acidic phospholipid phosphate content) are not affected by formalin fixation, the uronate and DNA contents are reduced in formalin-fixed bone while hydroxyproline content is elevated.
mesh_terms: D001842:Bone and Bones / Q000032:analysis* / Q000187:drug effects; D003094:Collagen / Q000032:analysis; D004247:DNA / Q000032:analysis; D005557:Formaldehyde / Q000494:pharmacology*; D005615:Freezing; D006652:Histological Techniques; D006801:Humans; D006909:Hydroxyproline / Q000032:analysis; D008055:Lipids / Q000032:analysis; D011509:Proteoglycans / Q000032:analysis; D013997:Time Factors; D014574:Uronic Acids / Q000032:analysis
chemical_list: D008055:Lipids; D011509:Proteoglycans; D014574:Uronic Acids; D005557:Formaldehyde; D003094:Collagen; D004247:DNA; D006909:Hydroxyproline