Assay of plasma endogenous inhibitor(s) of prostaglandin synthase using an enzyme incubation - radioimmunoassay method.
A simple, reliable method with validations is described for the routine measurement of endogenous inhibitor(s) of prostaglandin synthase in blood plasma. Appropriate dilutions of plasma samples are incubated with bovine seminal vesicle prostaglandin synthase, sodium arachidonate and reduced glutathione. Prostaglandin E2 production is then quantitated by radioimmunoassay. The relative inhibitory potency of plasma samples is determined by comparison of prostaglandin E2 content in sample incubations with that of control incubations containing no plasma dilution. Possible applications and the suitability of this methodological approach are discussed.
D000818:Animals; D000918:Antibody Specificity; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood; D016861:Cyclooxygenase Inhibitors; D015232:Dinoprostone; D005978:Glutathione / Q000097:blood; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D011458:Prostaglandins E / Q000097:blood; D011863:Radioimmunoassay / Q000379:methods; D012756:Sheep; D013696:Temperature; D013997:Time Factors
D001095:Arachidonic Acids; D016861:Cyclooxygenase Inhibitors; D011458:Prostaglandins E; D016718:Arachidonic Acid; D005978:Glutathione; D015232:Dinoprostone