Benzylamine oxidase in normal and atherosclerotic human aortae.
Assays of serum benzylamine oxidase (BzAO) have led some workers to postulate a relationship between elevated BzAO activity and diseases characterized by proliferating connective tissue. The present study was designed to determine whether BzAO activity of a cellular tissue is also affected. BzAO was assayed in homogenates of normal and atherosclerotic human aortae. Characterization done in normal aortae showed that BzAO is not a classical monoamine, diamine, polyamine, or lysyl oxidase, nor is it a ceruloplasmin. The enzyme is heat stable at 60 degrees C and is associated primarily with the microsomal fraction on density centrifugation. Compared with phenylethylamines and indoleamines, benzylamine is the best substrate. BzAO is sensitive to inhibition by hydrazines and chymotrypsin but not trypsin, and is insensitive to Triton X-100 and sulfhydryl-group blockade. BzAO activity of atherosclerotic plaque (expressed per gram wet weight or per milligram protein) was decreased markedly compared to that in adjacent, nonplaque regions and in normal aortae. However, on a per milligram DNA basis, the BzAO activity of plaque did not differ from that of nonplaque tissue. We conclude that there is a decreased cell population density in plaque, a contention supported by kinetic analysis. Plaque BzAO showed a decreased Vmax with no change in the Km of benzylamine compared with nonplaque tissue. Thus, if a relationship exists between BzAO activity and proliferating connective tissue, it is not apparent at the level of the cellular enzyme in atherosclerotic aortae of man.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001011:Aorta / Q000032:analysis / Q000201:enzymology*; D001161:Arteriosclerosis / Q000201:enzymology*; D001344:Autopsy; D001595:Benzylamine Oxidase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D001596:Benzylamines / Q000378:metabolism; D002458:Cell Fractionation; D004247:DNA / Q000032:analysis; D005260:Female; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008875:Middle Aged; D008995:Monoamine Oxidase / Q000378:metabolism*; D011506:Proteins / Q000032:analysis; D013379:Substrate Specificity
D001596:Benzylamines; D011506:Proteins; D004247:DNA; D001595:Benzylamine Oxidase; D008995:Monoamine Oxidase