title: Effect of 2-thiouracil on RNA and protein synthesis in synchronous and asynchronous infections of tobacco mosaic virus.
abstract: Examination of the effect of 2-thiouracil on tobacco mosaic virus (TMV) RNA and protein synthesis in synchronous and asynchronous systems of replication in tobacco leaves demonstrated that 2-thiouracil does not prevent synthesis of TMV RNA, as suggested by previous studies, but inhibits some earlier function. When added at different times after mechanical inoculation, 1 mM 2-thiouracil partially inhibited both viral RNA and protein synthesis, with greater inhibition when treatment began nearer the time of inoculation. In leaves systemically inoculated with TMV using a differential temperature inoculation procedure to synchronize the infection, 1 mM 2-thiouracil inhibited viral protein and RNA synthesis totally when treatment began within the first 4 h after initiation of replication, but not at all when treatment began at 12 h or later, even though earlier reports had shown that most RNA and protein synthesis occurred after 12 h.
mesh_terms: D012367:RNA, Viral / Q000096:biosynthesis*; D013696:Temperature; D013889:Thiouracil / Q000494:pharmacology*; D013997:Time Factors; D014027:Tobacco Mosaic Virus / Q000187:drug effects* / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; D013889:Thiouracil