title: RNA splicing in neurospora mitochondria: structure of the unspliced 35S precursor ribosomal RNA detected by psoralen cross-linking.
abstract: The structure of the unspliced 35S precursor rRNA of Neurospora mitochondria was studied by psoralen photochemical cross-linking. The results show that when the 35S RNA is cross-linked in ribonucleoprotein particles (RNPs) under appropriate conditions, the predominant configuration is a 2.2 kb intron loop which brings opposite splice sites into proximity; that the predominant secondary structural feature in the free RNA is a relatively large hairpin (length = 0.105 kb) in the center of the molecule at or near the 5' splice site; that the intron loop and the central hairpin are different configurations of sequences at or near the 5' splice site; and that the intron loop is stabilized by protein components of RNPs. Based on the structures detected by psoralen photochemical cross-linking, we propose a mechanism for the splicing of the Neurospora mitochondrial precursor rRNA. We propose further that certain features of this mechanism may be relevant to the splicing of other RNAs, including eucaryotic mRNAs.
mesh_terms: D001483:Base Sequence; D003432:Cross-Linking Reagents; D008928:Mitochondria / Q000032:analysis / Q000378:metabolism*; D009491:Neurospora / Q000378:metabolism; D009690:Nucleic Acid Conformation; D009694:Nucleic Acid Precursors / Q000378:metabolism*; D012326:RNA Splicing; D012331:RNA, Fungal / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000032:analysis; D014307:Trioxsalen / Q000031:analogs & derivatives
chemical_list: D003432:Cross-Linking Reagents; D009694:Nucleic Acid Precursors; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012261:Ribonucleoproteins; C018822:aminomethyltrioxsalen; D014307:Trioxsalen