Intracellular pH and glutathione levels in rabbit corneal endothelium following storage in moist chamber and MK medium.
Rabbit corneas were stored for up to 14 days at 4 C either as the whole eye in a moist chamber or as the isolated cornea in MK medium with HEPES buffer. The intracellular pH, the glutathione content, and its oxidation state were determined in the endothelial cells of fresh and stored tissue. The endothelial pH was found to be unchanged following storage of up to 7 days by either method, but after 14 days the pH rose slightly but statistically significantly in corneas stored by both techniques. The intracellular pH was similar in endothelia of those corneas stored in MK medium and of those stored as the whole eye in a moist chamber, for all time periods studied. The intracellular total and percent oxidized glutathione of the endothelium were increased by 50 and 180%, respectively, following 7 days of moist chamber storage. Over this time period there was a 50-fold increase in total glutathione content of the aqueous humor in the stored eyes. In contrast, corneas stored in MK medium for 7 days maintained intracellular total glutathione at levels similar to those of fresh corneas. A gradual but constant decrease in percent oxidized glutathione was observed with increasing length of storage. In terms of pH and glutathione content, the MK medium provided a much more stable environment for the stored cornea than did the aqueous humor in the stored eye.
D000818:Animals; D001082:Aqueous Humor / Q000032:analysis; D003315:Cornea / Q000032:analysis*; D004727:Endothelium / Q000032:analysis; D005978:Glutathione / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D009926:Organ Preservation / Q000379:methods*; D010084:Oxidation-Reduction; D011817:Rabbits; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*
D005978:Glutathione