title: Selective recovery of oral Capnocytophaga spp. with sheep blood agar containing bacitracin and polymyxin B.
abstract: On the basis of in vitro susceptibility testing of antibiotics, dyes, and other antimicrobial agents, we developed and evaluated a medium, TBBP, for the selective isolation of oral Capnocytophaga spp. TBBP medium consists of 4% Trypticase soy agar (BBL Microbiology Systems, Cockeysville, Md.), 5% sheep blood, 0.1% yeast extract, 50 micrograms of bacitracin per ml, and 100 micrograms of polymyxin B per ml. A total of 34 Capnocytophaga stock cultures grew well on TBBP medium. Except for some streptococcal strains, TBBP medium inhibited growth of all test stock culture isolates of common oral gram-positive and gram-negative species. In a clinical study of 15 deep periodontal pockets, TBBP medium demonstrated Capnocytophaga recoverability that was similar to or higher than that shown by a nonselective blood agar medium. Typical Capnocytophaga colonial morphology enabled us to readily distinguish this organism from the few other bacteria which could grow on TBBP medium.
mesh_terms: D000293:Adolescent; D000328:Adult; D000362:Agar; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001414:Bacitracin / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002206:Capnocytophaga / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D004396:Coloring Agents / Q000494:pharmacology; D003470:Culture Media; D041964:Cytophagaceae / Q000302:isolation & purification*; D006801:Humans; D008826:Microbial Sensitivity Tests; D010518:Periodontitis / Q000382:microbiology; D011112:Polymyxin B / Q000494:pharmacology; D012756:Sheep / Q000097:blood
chemical_list: D000900:Anti-Bacterial Agents; D004396:Coloring Agents; D003470:Culture Media; D001414:Bacitracin; D000362:Agar; D011112:Polymyxin B