The simultaneous use of Hanks' and Hepes buffers in the preparation of human leucocytes for SEM observation.
Leucocytes were isolated from the peripheral blood of normal individuals and prepared for SEM observation. The addition of the buffer Hanks and Hepes to the glutaraldehyde fixative was found to maintain the initial ambient pH throughout cell manipulation, and to allow satisfactory preservation of surface architecture.
D002021:Buffers; D005404:Fixatives; D005976:Glutaral; D006531:HEPES; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007962:Leukocytes / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D010879:Piperazines
D002021:Buffers; D005404:Fixatives; D010879:Piperazines; D006531:HEPES; D005976:Glutaral