title: A soybean lectin having 4-O-methyl-D-glucuronic acid specificity.
abstract: Evidence is presented for the presence of a new lectin activity in soybean seeds [Glycine max (L.) Merrill] that has specificity towards the 4-O-methyl-D-glucurono-L-rhamnan exopolysaccharide produced by certain strains of Rhizobium japonicum. Bacterial agglutination and precipitin reactions revealed the lectin activity in phosphate-buffered saline extracts of seeds of all cultivars tested, including the "lectinless" varieties. Reaction of such extracts with carbohydrate haptens demonstrated that the specificity of the binding was towards 4-O-methyl-D-glucuronic acid, D-glucuronic acid and their methyl glycosides.
mesh_terms: D001666:Binding Sites, Antibody; D055598:Chemical Phenomena; D002621:Chemistry; D005965:Glucuronates / Q000276:immunology*; D037102:Lectins / Q000032:analysis*; D037121:Plant Lectins; D011135:Polysaccharides, Bacterial; D012231:Rhizobium; D030262:Soybean Proteins; D010835:Phytohemagglutinins
chemical_list: D005965:Glucuronates; D037102:Lectins; D037121:Plant Lectins; D011135:Polysaccharides, Bacterial; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins; C036035:4-O-methylglucuronic acid