Evidence that certain peripheral anti-acetylcholine receptor antibodies do not interact with brain BuTX binding sites.
The cross-reactivity of rat brain alpha-bungarotoxin (BuTX) binding sites with peripheral anti-acetylcholine receptor (nAChR) polyclonal and monoclonal antibodies was investigated in Triton extracts of several brain areas and purified whole brain putative receptors using radioimmunoassays and IgG/CNBr-Sepharose 4B immunoaffinity chromatography. The effects of the chronic intracerebral administration of antibodies on [125I]BuTX binding and food and water intake were also investigated. It was concluded that brain BuTX sites do not share antigenic determinants with peripheral nAChRs. Additionally, no evidence was found to support the suggestions that anti-nAChR antibodies affect food and water intake, or that chronic administration of antibodies alters [125I]BuTX binding in the hypothalamus of rats.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D001921:Brain / Q000378:metabolism*; D002038:Bungarotoxins / Q000378:metabolism*; D051381:Rats; D011950:Receptors, Cholinergic / Q000276:immunology* / Q000378:metabolism; D011978:Receptors, Nicotinic; D014018:Tissue Distribution; D064569:alpha7 Nicotinic Acetylcholine Receptor
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D002038:Bungarotoxins; C579165:Chrna7 protein, rat; D011950:Receptors, Cholinergic; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor