ADP-ribosylation in in vitro systems synthesizing adenovirus DNA.
Two systems utilizing extracts derived from nuclei of adenovirus-infected cells which synthesize adenovirus DNA in vitro were analysed for indications of ADP-ribosylation of virus proteins. On incubation with [32P]NAD or [14C]NAD, modification of the adenovirus T antigen could be demonstrated in one of these systems. ADP-ribosylation of adenovirus core proteins V and VII could also be demonstrated with both nuclear extracts. However, using 3-aminobenzamide, a specific inhibitor of poly(ADP-ribose) polymerase, there was no evidence either in vivo or in vitro that ADP-ribosylation played a critical role in the replication process.
D000246:Adenosine Diphosphate Ribose / Q000032:analysis*; D000256:Adenoviridae / Q000235:genetics*; D000956:Antigens, Viral / Q000032:analysis; D000957:Antigens, Viral, Tumor; D001549:Benzamides / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D004279:DNA, Viral / Q000032:analysis* / Q000096:biosynthesis; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D009702:Nucleoside Diphosphate Sugars / Q000032:analysis*; D014758:Viral Core Proteins; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication / Q000187:drug effects
D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor; D001549:Benzamides; D004279:DNA, Viral; D009702:Nucleoside Diphosphate Sugars; D014758:Viral Core Proteins; D014764:Viral Proteins; D000246:Adenosine Diphosphate Ribose; C025160:3-aminobenzamide