Comparative metabolism and macromolecular binding of benzo[a]pyrene in explant cultures of human bladder, skin, bronchus and esophagus from eight individuals.
Benzo[a]pyrene (B[a]P) metabolism and macromolecular binding have been studied in explants from 4 tissues, bladder, skin, bronchus and esophagus from 8 donors sampled within 4 h after death. Explants were incubated with [3H]benzo[a]pyrene for 24 h, then the metabolites extracted and analyzed by high-pressure liquid chromatography. Fibroblasts were grown from explants from 2 patients and also incubated with B[a]P. Metabolite profiles were qualitatively the same for explants and fibroblasts with similar product ratios, although fibroblasts were less active in B[a]P metabolism. DNA binding studies showed a broad variance between patients and tissues with the relative distribution being widest in bladder, followed by skin, bronchus and esophagus, respectively.
D001580:Benzopyrenes / Q000378:metabolism*; D001980:Bronchi / Q000378:metabolism*; D046508:Culture Techniques; D004947:Esophagus / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D012867:Skin / Q000378:metabolism*; D014018:Tissue Distribution; D001743:Urinary Bladder / Q000378:metabolism*
D001580:Benzopyrenes