[Determination of free and conjugated cis-3,3,5-trimethylcyclohexanol in plasma and urine].
A gas chromatographic procedure was developed to determine free and conjugated cis-3,3,5-trimethylcylcohexanol in plasma and urine. The sample is extracted with dichloromethane when free cis-3,3,5-trimethylcyclohexanol is determined, or with hexane after enzymatic hydrolysis, when conjugated cis-3,3,5-trimethylcyclohexanol is determined. An aliquot of the organic extract is injected into a stainless-steel column (packed with Carbowax 20M, 15% on Chromosorb W AW 100-120 mesh) and detected with a flame ionization detector. Extraction recovery from plasma and urine was almost 100% and the limit of quantification was fixed at 100 ng/ml plasma or urine. The procedure was evaluated in a pharmacokinetic study of cyclandelate and its metabolite cis-3,3,5-trimethylcyclohexanol.
D000818:Animals; D001711:Biotransformation; D003495:Cyclandelate / Q000378:metabolism; D003511:Cyclohexanols / Q000032:analysis* / Q000097:blood / Q000652:urine; D005410:Flame Ionization; D007536:Isomerism; D007700:Kinetics; D011817:Rabbits
D003511:Cyclohexanols; C037269:3,3,5-trimethylcyclohexanol; D003495:Cyclandelate
Dosage plasmatique et urinaire du 3,3,5-triméthylcyclohexanol-cis libre et conjugué.