[Membrane potential during Ca++ transport and AMP deamination in vesicles of the sarcoplasmic reticulum].
Transport of Ca2+ to vesicles of the sarcoplasmic reticulum and its subsequent removal by AMP is accompanied by fluorescence intensification and quenching, respectively, of the charged potentially-dependent probes of 1-anilino-8-naphthalenesulphonate and 3,3'-dipropilthiodicarbocyanin as well as 4-dimethylaminochalcone responding to conformation changes in membranes. The transmembrane electrophoretic distribution of synthetic fat-soluble ions of phenyldicarboundecaborane and tetraphenylphosphonium during Ca2+ accumulation is characterized by uptake of phenyldicarboundecaborane with the subsequent removal by protonophore of m-cholorocarbonylcyanide phenylhydrazone or AMP; the uptake of tetraphenylphosphonium is absent. This testifies to the fact that the Ca2+ transport in vesicles is accompanied by the formation of a membrane potential with a positive sign inside the vesicles.
D000249:Adenosine Monophosphate / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D003641:Deamination; D005456:Fluorescent Dyes; D008564:Membrane Potentials; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
D005456:Fluorescent Dyes; D000249:Adenosine Monophosphate; D002118:Calcium
Membrannyĭ potentsial pri transporte Ca++ i dezaminirovanii AMP v vezikulakh sarkoplazmaticheskogo retikuluma.