[Inhibition by dipropyl acetate and its structural analogs of the glycine synthase system in liver and brain mitochondria].
The effect of the anticonvulsive di-n-propylacetate (DPA) and some structural analogous, all branched-chain organic acids, on the glycine cleavage system in rat liver and brain mitochondria has been studied. DPA and n-propyl, prop-2-enylacetic, 4,4-di-n-propylbutyric, 2-ethyl, 3-methylvaleric acids inhibit this enzymatic activity corresponding the greatest degree of inhibition to DPA (70% and 86% inhibition in liver and brain mitochondria respectively at 10 mM DPA). 2-methyl, 3-ethyl caproic acid has an inhibitory effect on glycine cleavage activity only in liver mitochondria, but showing no effect on this activity in brain mitochondria. The inhibitory effect of DPA can explain the hyperglycinemia and hyperglycinuria in DPA-treated patients. This effect may provide also an experimental approach for the study of biochemical and pathogenia mechanisms of non-ketotic hyperglycinemia.
D000594:Amino Acid Oxidoreductases; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D001921:Brain / Q000201:enzymology*; D002352:Carrier Proteins; D004827:Epilepsy / Q000097:blood; D005998:Glycine / Q000097:blood; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology*; D009097:Multienzyme Complexes; D051381:Rats; D011919:Rats, Inbred Strains; D014166:Transferases / Q000037:antagonists & inhibitors*; D014635:Valproic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*
D000927:Anticonvulsants; D002352:Carrier Proteins; D009097:Multienzyme Complexes; C064525:glycine cleavage system; D014635:Valproic Acid; D000594:Amino Acid Oxidoreductases; D014166:Transferases; D005998:Glycine
Inhibición por dipropilacetato y análogos estructurales del sistema glicina sintasa en mitocondrias de hígado y de cerebro.