Heterogeneity of lymphokine production by murine T-lymphocyte clones.
The production of lymphokines by alloreactive murine T-lymphocyte clones was investigated. Clones derived by limiting dilution or by micromanipulation were stimulated with T-cell-depleted irradiated allogeneic spleen cells, and the resulting supernatants were assayed for interleukin-2 (IL-2), interferon-gamma (gamma-IFN), macrophage-activating factor (MAF), B-cell helper factor (BHF) or granulocyte/macrophage colony-stimulating factor (GM-CSF). Lymphokine production was found to be heterogeneous at the clonal level, with both cytolytic and non-cytolytic clones being able to produce some (or all) of the factors tested. Comparison of a large number of independently derived clonal supernatants allowed the separation of IL-2, BHF and GM-CSF from each other and from MAF and gamma-IFN; however, no dissociation between the latter 2 lymphokines was observed.
D000818:Animals; D002999:Clone Cells / Q000276:immunology; D003115:Colony-Stimulating Factors / Q000096:biosynthesis; D006133:Growth Substances / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis* / Q000235:genetics; D016215:Macrophage-Activating Factors; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007376:Interleukin-2; D008222:Lymphokines; D016215:Macrophage-Activating Factors; D015847:Interleukin-4; D007371:Interferon-gamma