Cytochemical and competitive protein binding assays for estrogen receptor in breast disease. A comparative study of 62 cases.
Estrogen receptor content of breast lesions was estimated using a fluorescent cytochemical technique and a competitive protein binding assay. Of 48 cancers examined, an equal proportion contained significant quantities of receptor by either method (62.5%). The concordance between methods for individual patients was also 62.5%. A greater proportion (26%) of patients younger than 45 years of age had receptor-positive cancers using the cytochemical method than were found by the biochemical method (10%). Benign breast disease was also studied using the fluorescent cytochemical method. A greater proportion of lesions containing estrogen receptors was found compared with that cited in the literature for the competitive protein-binding assay. Because of the methodologic simplicity of the fluorescent cytochemical method, further study for routine use is indicated.
D000232:Adenofibroma / Q000032:analysis / Q000378:metabolism; D000328:Adult; D001943:Breast Neoplasms / Q000032:analysis* / Q000378:metabolism; D005260:Female; D005348:Fibrocystic Breast Disease / Q000032:analysis / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D008722:Methods; D008875:Middle Aged; D011485:Protein Binding; D011960:Receptors, Estrogen / Q000032:analysis*
D011960:Receptors, Estrogen