title: Configuration at C-25 in 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestan-26-oic acid isolated from human bile.
abstract: This report describes the isolation of the natural isomer of 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestan-26-oic acid from human bile by a method that retains the configuration at C-25. The stereochemistry at C-25 in this bile acid was defined as 25R by a direct comparison with standard (25R)3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestan-26-oic acid.
mesh_terms: D001646:Bile / Q000032:analysis*; D002793:Cholic Acids / Q000302:isolation & purification*; D002849:Chromatography, Gas / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D006801:Humans; D008968:Molecular Conformation; D013237:Stereoisomerism
chemical_list: D002793:Cholic Acids; C003681:3,7,12-trihydroxycoprostanic acid