Synthesis of D-cysteine-related amino acids by 3-chloro-D-alanine chloride-lyase of Pseudomonas putida CR 1-1.
Using the beta-replacement reaction of 3-chloro-D-alanine chloride-lyase from Pseudomonas putida CR 1-1, S-methyl-, S-ethyl-, S-n-propyl-, S-n-butyl-, S-phenyl-, S-benzyl-, S-(2,3-dihydroxypropyl)- and S-(2-hydroxyethyl)-D-cysteine were synthesized from 3-chloro-D-alanine and methyl-, ethyl-, n-propyl-, n-butyl-, phenyl-, benzyl-mercaptans, alpha-thioglycerol and 2-mercaptoethanol, respectively. The enzymatically synthesized D-cysteine-related amino acids were isolated from the large scale reaction mixture and identified physicochemically.
D003545:Cysteine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D007700:Kinetics; D008190:Lyases / Q000502:physiology*; D011549:Pseudomonas / Q000201:enzymology*; D013438:Sulfhydryl Compounds / Q000378:metabolism
D013438:Sulfhydryl Compounds; D008190:Lyases; C031729:3-chloro-D-alanine dehydrochlorinase; D003545:Cysteine