An improved method for isolation of tritium-labelled auto-antigen I of the human sperm membrane.
The isolation of tritium-labelled auto-antigen I of the human sperm membrane can be improved by including a gel chromatography step on a Sephadex G-200 column in the isolation procedure. The antigen was isolated from tritium-labelled spermatozoa by detergent solubilization, lectin affinity chromatography, gel chromatography and indirect immunoprecipitation. The gel chromatography removed the majority of material which binds non-specifically in the indirect immunoprecipitation and which had complicated the electrophoretic analysis of the isolated antigen. The auto-antigen was found to have a molecular weight greater than 150,000 and to exhibit an electrophoresis pattern of six polypeptide chains with molecular weights of 21,000, 32,000, 41,000, two around 77,000, and 120,000.
D000941:Antigens / Q000302:isolation & purification*; D001323:Autoantibodies / Q000276:immunology; D001324:Autoantigens / Q000302:isolation & purification*; D002462:Cell Membrane / Q000276:immunology; D002850:Chromatography, Gel; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000276:immunology; D008970:Molecular Weight; D013094:Spermatozoa / Q000276:immunology*; D014316:Tritium
D000941:Antigens; D001323:Autoantibodies; D001324:Autoantigens; D006023:Glycoproteins; D008565:Membrane Proteins; D014316:Tritium