The effects of cell-culture density on cell inactivation by benzo[a]pyrene-4,5-oxide.
Initial survival levels of CHO-K1 monolayers following exposure to benzo[a]pyrene-4,5-oxide were found to depend upon the culture density of exponential-phase cells during the exposure portion of the experiment. A continuous increase in the surviving fraction (measured by cloning ability) was observed when culture treatment densities were above 2 x 10(4) cells/cm2 (approximately 15% of maximum density). This effect was not observed with ultraviolet-irradiated cells nor due to increased repair times, frequently observed in plateau (density)-inhibited cultures. Furthermore, treated cells unable to grow at cloning densities (8-15 cells/cm2) continued to grow and divide for at least 9 population doublings following exposure provided post-exposure culture densities were maintained at 6.6 x 10(3) cells/cm2 or greater.
D000818:Animals; D001580:Benzopyrenes / Q000494:pharmacology*; D002452:Cell Count; D002455:Cell Division; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D003260:Contact Inhibition; D006224:Cricetinae; D003412:Cricetulus; D014466:Ultraviolet Rays
D001580:Benzopyrenes; C023393:benzo(a)pyrene 7,8-oxide