Dynamics and topographical distribution of surface glycoproteins during myoblast fusion: a resonance energy transfer study.
We have investigated changes in topography and lateral translational mobility of concanavalin A (Con A) receptors on the surface of cultured chick muscle cells during the period of myoblast fusion. A temporal correlation between these phenomena and the alteration in membrane fluidity known to occur during this time period is established. Receptor topography and mobility are studied by means of a resonance energy transfer technique employing pyrene- and FITC-Con A conjugates. All measurements are performed through a microscope on single cells. Our results reveal that during the period of myoblast fusion Con A receptors undergo a dramatic redistribution on the cell surface. Furthermore, our data suggest that the changes in membrane fluidity observed during muscle differentiation serve to modulate the lateral mobility of these receptors.
D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism*; D002642:Chick Embryo; D003078:Colchicine / Q000494:pharmacology; D003208:Concanavalin A / Q000031:analogs & derivatives; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008560:Membrane Fluidity; D009132:Muscles / Q000166:cytology*; D015739:Nocodazole; D011721:Pyrenes; D011952:Receptors, Concanavalin A / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013861:Thiocyanates
D001562:Benzimidazoles; D005452:Fluoresceins; D005456:Fluorescent Dyes; D011721:Pyrenes; D011952:Receptors, Concanavalin A; D013861:Thiocyanates; D003208:Concanavalin A; D016650:Fluorescein-5-isothiocyanate; D015739:Nocodazole; D003078:Colchicine