title: Modulation of human immune responsiveness in vitro by auranofin.
abstract: The effect of auranofin (AF) on in vitro correlates of human immune responsiveness was examined. AF inhibited mitogen induced human lymphocyte proliferation and the generation of immunoglobulin secreting cells in a concentration dependent manner. The inhibition was most effective when AF was present from the initiation of culture indicating that this drug blocked a critical early step in lymphocyte activation. Marked inhibition of mitogen responsiveness was observed as a result of a 1-h preincubation with AF. The brief preincubation with low concentrations of AF (0.3 micrograms/ml) resulted in a selective inhibition of the accessory function of monocytes but had no effect on potential lymphocyte responsiveness. Preincubation with higher concentrations of AF (greater than 0.6 micrograms/ml) resulted in a more non-specific inhibition of both monocyte and lymphocyte function. These data support the conclusion that AF may function as an immunosuppressive agent.
mesh_terms: D000917:Antibody Formation / Q000187:drug effects*; D001310:Auranofin; D006051:Aurothioglucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D002455:Cell Division; D006046:Gold / Q000031:analogs & derivatives*; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology; D008934:Mitogens / Q000276:immunology; D009000:Monocytes / Q000502:physiology; D013997:Time Factors
chemical_list: D007136:Immunoglobulins; D008934:Mitogens; D006051:Aurothioglucose; D001310:Auranofin; D006046:Gold