title: Reverse phase liquid chromatographic determination of sulfathiazole residues in honey.
abstract: Sulfathiazole residues were extracted from honey by homogenizing samples in acetone, filtering, and then evaporating the acetone under nitrogen at 40 degrees C. The remaining extract was transferred to a separatory funnel with 1N HCl and ethyl ether. An aliquot of the retained acid layer was screened by using the Bratton-Marshall reaction. If the test was positive, the remaining portion was analyzed directly through a mu Bondapak phenyl column monitored by a UV detector at 254 nm. The mobile phase was potassium phosphate monobasic in 10% acetonitrile adjusted to pH 3.0. Time for elution was 13 min. Average recoveries were 78% at the 0.1 ppm spiking level and 68% at the 1.0 ppm level. The minimum detectable amount was 0.06 ppm based on a spiked sample extract.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods; D006722:Honey / Q000032:analysis*; D008832:Microchemistry; D013432:Sulfathiazoles / Q000302:isolation & purification*
chemical_list: D013432:Sulfathiazoles