title: Microvascular abnormalities in ethylnitrosourea (ENU)-induced rat brain tumors: structural basis for altered blood-brain barrier function.
abstract: The fine structure, histometric characteristics, and permeability of microvessels were studied by electron microscopy in normal and in ethylnitrosourea (ENU)-induced glioma tissue from rats, using horseradish peroxidase (HRP) as a tracer. The tumor vessels were classified into (1) capillary buds (Type I); (2) round small to large capillaries (Type II); (3) sinusoidal or venule-like microvessels (Type III), and (4) abnormal arteriole-like microvessels (Type IV). All endothelial cells, basement membranes and periendothelial cells in the tumor tissue demonstrated changes in structure. The most striking alterations occurred in the endothelial cells; there were abnormal endothelial tight junctions, altered pinocytotic activity, and thickening. In the tracer study, the reaction product of HRP was present around some sinusoidal or venule-like microvessels (Type III) and extended to the widened extracellular spaces around the microvessels. The endothelial cells of Type III microvessels showed decreased nuclear and mitochondrial fractions, and increased euchromatin content and a rough endoplasmic reticulum fraction. The pinocytotic vesicles with the HRP reaction product in the endothelial cells were not increased in number. Fenestrations and gaps of the endothelial cells were observed. These alterations of the endothelial cells of sinusoidal or venule-like microvessels (Type III) are considered to be the main cause of breakdown of the blood-brain barrier in this tumor.
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects*; D001932:Brain Neoplasms / Q000139:chemically induced* / Q000648:ultrastructure; D002199:Capillary Permeability / Q000187:drug effects; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D004727:Endothelium / Q000648:ultrastructure; D005038:Ethylnitrosourea / Q000633:toxicity*; D005910:Glioma / Q000139:chemically induced* / Q000648:ultrastructure; D006735:Horseradish Peroxidase; D007365:Intercellular Junctions / Q000648:ultrastructure; D008833:Microcirculation / Q000648:ultrastructure; D008854:Microscopy, Electron; D009374:Neoplasms, Experimental / Q000139:chemically induced; D009607:Nitrosourea Compounds / Q000633:toxicity*; D010873:Pinocytosis / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D009607:Nitrosourea Compounds; D006735:Horseradish Peroxidase; D005038:Ethylnitrosourea