[Effect of specific inhibition of DNA replication by aphidicolin on primary differentiation of preimplantation mouse embryos].
Aphidicolin inhibits cleavage at every stage of preimplantation of Mouse embryos without affecting compaction. Blastulation is initiated only if the treatment is applied at the late 8-cell stage. It is concluded that the S phase of the 4th cell cycle might be critical for the commitment of the embryo for blastocyst formation. The 4th cycle thus corresponds to a "quantal cell cycle". Moreover, aphidicolin induces premature cavitation which occurs even when the embryos have not yet initiated cytokinesis.
D000818:Animals; D016590:Aphidicolin; D001755:Blastocyst / Q000187:drug effects / Q000502:physiology*; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors*; D004261:DNA Replication / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology*; D005260:Female; D051379:Mice; D019384:Nucleic Acid Synthesis Inhibitors
D004224:Diterpenes; D019384:Nucleic Acid Synthesis Inhibitors; D016590:Aphidicolin; D004257:DNA Polymerase II
Effet de l'inhibition spécifique de la réplication de l'ADN par l'aphidicoline sur la différenciation primaire de l'auf de souris en préimplantation.