Preferential modification of the inverted repeat DNA of tetrahymena by N-methyl-N'-nitro-N-nitrosoguanidine.
DNA was prepared from Tetrahymena pyriformis, that was cultured for 2 h in medium containing chemical carcinogens. After denaturation of DNA in alkaline solution, DNA was quickly renatured below 10(-3) Cot and the double-strand DNA (the inverted repeat DNA) was separated from the single-strand DNA on a column of hydroxyapatite. N-Methyl-N' -nitro-N-nitrosoguanidine and methyl methanesulfonate preferentially bound in vivo to the inverted repeat DNA rather than other DNA components which were recovered as single-strand DNA.
D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism; D008741:Methyl Methanesulfonate / Q000494:pharmacology*; D008745:Methylation; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D012091:Repetitive Sequences, Nucleic Acid / Q000187:drug effects*; D013769:Tetrahymena pyriformis / Q000187:drug effects / Q000235:genetics
D001580:Benzopyrenes; D008769:Methylnitronitrosoguanidine; D008741:Methyl Methanesulfonate