title: Structural analysis of fibrinogen synthesized by cultured chicken hepatocytes in the presence or absence of dexamethasone.
abstract: Hepatocyte monolayers, derived from chick embryos and cultured in chemically defined medium without hormones, synthesize and secrete fibrinogen that resembles chicken plasma fibrinogen immunochemically and structurally. Addition of a synthetic glucocorticoid, dexamethasone, to the cultured cells resulted in an appreciable and relatively selective increase in fibrinogen synthesis. Autoradiography of fibrinogen that had been metabolically labelled with [35S]methionine and then subjected to SDS-polyacrylamide gel electrophoresis, unreduced or under disulfide-reducing conditions, revealed that only dimeric forms of fibrinogen, containing undegraded A alpha, B beta, and gamma chains, were secreted under stimulated and unstimulated culture conditions.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003907:Dexamethasone / Q000494:pharmacology*; D004790:Enzyme Induction; D005340:Fibrinogen / Q000096:biosynthesis* / Q000302:isolation & purification; D008099:Liver / Q000187:drug effects / Q000201:enzymology*
chemical_list: D003907:Dexamethasone; D005340:Fibrinogen