Bovine growth hormone fragment (1-133) has in-vitro somatomedin-like activity.
Thrombin digestion of bovine growth hormone (1-191) resulted in cleavage of the peptide bond between amino acid residues 133 and 134. Native growth hormone and purified peptides (1-133) and (134-191) were assayed for somatomedin-like activity. Peptide (1-133), ranging in concentration from 0.15-15 nmol/l, stimulated in-vitro uptake of [3H]thymidine by rat costal cartilage. None of the other peptides was biologically active.
D000818:Animals; D002417:Cattle; D004247:DNA / Q000096:biosynthesis; D013006:Growth Hormone / Q000378:metabolism*; D066298:In Vitro Techniques; D010446:Peptide Fragments / Q000302:isolation & purification / Q000378:metabolism*; D013002:Somatomedins / Q000378:metabolism*
D010446:Peptide Fragments; D013002:Somatomedins; D013006:Growth Hormone; D004247:DNA