[A protease of the external membrane of Escherichia coli sensitive to environmental conditions. Its relations with the expression of envZ gene].
A mutant strain (called Cpr), devoid of the outer membrane protease that cleaves colicin A, has been isolated. The location on the genetic map of the cpr locus as well as its pleiotropic effects concerning chiefly: the protein composition of the outer membrane, sensitivity to phages and colicins, alteration in protease activity (cpr), are very similar to the location on the genetic map and to phenotypic properties observed in strains tpo, perA or envZ which are altered in the ompB locus. Conditions resulting in inhibition of the colicin A protease activity also result in transcriptional regulation of OmpF, OmpC and LamB proteins synthesis. The possibility for this protease to be an osmosensor of the cell's external environment is discussed.
D002462:Cell Membrane / Q000201:enzymology; D002874:Chromosome Mapping; D003087:Colicins / Q000378:metabolism; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D009154:Mutation; D009994:Osmolar Concentration; D010447:Peptide Hydrolases / Q000235:genetics* / Q000378:metabolism
D003087:Colicins; D003470:Culture Media; D010447:Peptide Hydrolases
Une protéase de la membrane externe d'Escherichia coli sensible aux conditions d'environnement. Ses relations avec l'expression du gène envZ.