Carbachol-methohexitone interactions at the chick neuromuscular junction: experiments with the moving fluid electrode technique.
The moving fluid electrode was used to record simultaneously the depolarization and contracture responses produced by drug action. Carbachol (CCh) (5.5 X 10(-6)-5.5 X 10(-4)M) acetylcholine (ACh) (5.5 X 10(-6)-1.1 X 10(-2) M) produced large depolarization and contracture responses in the isolated chick biventer cervicis (BVC) muscle. These responses were greatly reduced by methohexitone (8.8 X 10(-5) M). The concentration-response curves for ACh and carbachol in the control Krebs solution were shifted in the presence of methohexitone to the right in a non-competitive manner. The present results indicate that both ACh and carbachol act postjunctionally by depolarizing the membrane and producing contractures in the chick biventer cervicis muscle.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D002217:Carbachol / Q000494:pharmacology*; D002645:Chickens; D004347:Drug Interactions; D004566:Electrodes; D066298:In Vitro Techniques; D008723:Methohexital / Q000494:pharmacology*; D009119:Muscle Contraction / Q000187:drug effects; D009469:Neuromuscular Junction / Q000187:drug effects*; D010830:Physostigmine / Q000494:pharmacology
D002217:Carbachol; D010830:Physostigmine; D008723:Methohexital; D000109:Acetylcholine