Studies on a new antibiotic M-92 produced by Micromonospora. IV. Bactericidal action of the component VA-2.
The action of VA-2, the most active component of antibiotic M-92, against S. aureus is bactericidal but not bacteriolytic. The bactericidal action is markedly affected by incubation temperature, whether bacterial cells are prolific or resting. The bactericidal kinetics of VA-2 is biphasic, since addition of VA-2 caused rapid and straight decrease in viability curve and reached a plateau after several minutes. The bactericidal activity of VA-2 is blocked by 2,4-dinitrophenol. Alike to many membrane-active bacteriocins, VA-2 seems to exert its action through two stages.
D019297:2,4-Dinitrophenol; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D004140:Dinitrophenols / Q000494:pharmacology; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008847:Micromonospora / Q000378:metabolism*; D009285:Naphthoquinones / Q000096:biosynthesis; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
D000900:Anti-Bacterial Agents; D004140:Dinitrophenols; D009285:Naphthoquinones; C036968:M 92; D019297:2,4-Dinitrophenol