Hepatic and plasma lysosomal enzyme activity in shock-like state following administration of polysaccharide-protein complex isolated from Candida albicans.
Polysaccharide-protein complex (PPC) derived from the cell walls of Candida albicans exerts several pathophysiological effects in laboratory animals similar to the symptoms of shock found at acute disseminated candidiasis. The present study evaluated the presumed fragility of hepatic lysosomes in rats in shock-like state following a single dose of PPC (25 mg/kg) administered intravenously, in terms of temporal changes in lysosomal enzymatic activity (cathepsin D, N-acetylglucosaminidase) in four sedimentable fractions and supernatant of liver homogenate after differential centrifugation, as well as in plasma. A reduction of sedimentable lysosomal activity (13,000 X g) was observed during the first hours (3-5 h) after administration of PPC with both lysosomal hydrolases, accompanied by a concomitant increase in the unsedimentable activity that represented the enzymatic activity released from disrupted lysosomes. Plasma levels of cathepsin D and N-acetylgucosaminidase had the same pattern of changes, reaching the maximum level 2-3 h after administration of PPC with a tendency of returning to the control levels by 12 h. Thus PPC from Candida albicans appears to have similar effects upon integrity of hepatic lysosomes as bacterial endotoxins have by labilizing the lysosomal membrane.
D000118:Acetylglucosaminidase / Q000097:blood / Q000378:metabolism; D000818:Animals; D002402:Cathepsin D; D002403:Cathepsins / Q000097:blood / Q000378:metabolism; D046508:Culture Techniques; D006023:Glycoproteins / Q000008:administration & dosage / Q000009:adverse effects*; D007275:Injections, Intravenous; D036341:Intercellular Signaling Peptides and Proteins; D008099:Liver / Q000648:ultrastructure*; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012772:Shock, Septic / Q000201:enzymology*
D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; C028196:polysaccharide-protein complex, Candida; D000118:Acetylglucosaminidase; D002403:Cathepsins; D002402:Cathepsin D