Enflurane, isoflurane, and methoxyflurane metabolism in rat hepatic microsomes from ethanol-treated animals.
The effects of ethanol on the metabolism of enflurane, isoflurane, and methoxyflurane were investigated to determine if alterations in biotransformation of these agents occur as a result of this treatment. In vitro incubations of hepatic microsomes from rats pretreated with 10 days' ethanol vapor inhalation revealed a fourfold increase in inorganic fluoride from enflurane when compared with incubations of microsomes from unpretreated rats and from phenobarbital-pretreated rats. Isoflurane, while metabolized to a lesser extent than enflurane, showed a similar stimulation of metabolism. Methoxyflurane, while metabolized to a greater extent than either enflurane or isoflurane, had lesser fluoride production by the microsomes from ethanol-pretreated rats than microsomes from phenobarbital-pretreated rats, but greater fluoride production than that found in microsomes from unpretreated rats. Ethanol pretreatment did not alter the levels of cytochrome P-450 which is the enzyme responsible for such metabolism. This suggests that the altered metabolism involves either a specific P-450 isozyme or an unidentified enzyme.
D000777:Anesthetics / Q000378:metabolism*; D000818:Animals; D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004737:Enflurane / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D005459:Fluorides / Q000378:metabolism; D007530:Isoflurane / Q000378:metabolism; D008297:Male; D008733:Methoxyflurane / Q000378:metabolism; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000777:Anesthetics; D008733:Methoxyflurane; D000431:Ethanol; D003577:Cytochrome P-450 Enzyme System; D004737:Enflurane; D007530:Isoflurane; D005459:Fluorides