Human c-Ki-ras2 proto-oncogene on chromosome 12.
A human colonic adenocarcinoma transforming gene, recently identified as a cellular homolog of the Kirsten sarcoma gene (v-ras), was used to assign the human cellular Kirsten ras2 gene to chromosome 12 by the Southern hybridization method. A single 640 base-pair Eco RI--Hind III fragment of the transforming gene, isolated by DNA transfection and molecular cloning, can detect a single Eco RI fragment (2.9 kilobase pairs) of DNA from phenotypically normal cells. The data suggest a constant chromosomal location of c-Ki-ras2.
D000230:Adenocarcinoma / Q000235:genetics; D002874:Chromosome Mapping; D002906:Chromosomes, Human, 6-12 and X; D003110:Colonic Neoplasms / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D007708:Kirsten murine sarcoma virus / Q000235:genetics; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D000090063:Proto-Oncogene Mas