Influence of diets on acyl-CoA:cholesterol acyltransferase and on acyl-CoA:retinol acyltransferase in villous and crypt cells from rat small intestinal mucosa and in the liver.
Cholesterol and retinol are both esterified with long-chain fatty acid within the mucosal cells of the small intestine. The reactions are catalyzed by microsomal acyl-CoA:cholesterol and acyl-CoA:retinol acyltransferases (EC 2.3.1.26, and EC 2.3.1.-, respectively). To gain more insight into the physiological importance of these acyltransferases, they were studied in villous and crypt cells from rats either fasting or on diets which varied in fat and cholesterol content. Both enzymes had a higher activity in villous than in crypt cells. The activities in villous cells varied with feeding and fasting and the composition of diet when the animals were killed postprandially. Acyl-CoA:cholesterol acyltransferase activity went up upon cholesterol feeding whereas retinol acyltransferase in the mucosa was reduced by high-fat diets. The liver cholesterol acyltransferase activity varied with diet, it increased with both cholesterol and fat feeding, whereas retinol acyltransferase activity remained relatively constant. The results obtained suggest that different diets are of importance for cholesterol and retinol acyltransferase activities both in the intestinal mucosa and in the liver. The variation in activities of the two acyltransferases suggests that they may be different enzymes.
D000217:Acyltransferases / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D002791:Cholesterol, Dietary / Q000494:pharmacology; D004032:Diet; D005215:Fasting; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000201:enzymology*; D007421:Intestine, Small / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D051142:Retinol O-Fatty-Acyltransferase; D002785:Sterol O-Acyltransferase / Q000378:metabolism* / Q000502:physiology; D014801:Vitamin A / Q000494:pharmacology
D002791:Cholesterol, Dietary; D014801:Vitamin A; D000217:Acyltransferases; D002785:Sterol O-Acyltransferase; D051142:Retinol O-Fatty-Acyltransferase