Reversal of hyperglycemia by intrasplenic transplantation of 4-week-cultured allogeneic mouse islets.
Culture of isolated mouse pancreatic islets in RPMI 1640 for 4 wk with maintenance of specific functions is possible, although a considerable reduction of the number of surviving islets was observed. Culture in medium TC 199 resulted in a lower islet loss, but such islets were functionally less competent. Noncultured C57BL/6J (H-2b) islets were rapidly rejected when allografted intrasplenically into alloxan-diabetic but nonimmunosuppressed C57BL/KsJ (H-2d) mice. By contrast, the immunogenicity of C57BL/6J islets cultured for 4 wk at 37 degrees C in RPMI 1640 and an air-carbon dioxide atmosphere was markedly reduced as evidenced by their ability to partly or completely normalize the hyperglycemia of nonimmunosuppressed alloxan-diabetic C57BL/KsJ mice for several weeks. Both intact islets and islets undergoing rejection were found in the spleens. The present data suggest that in vitro culture for 4 wk may reduce the immunogenicity of the islets, although a complete suppression is not achieved.
D000818:Animals; D003470:Culture Media; D046508:Culture Techniques; D003921:Diabetes Mellitus, Experimental / Q000628:therapy*; D005260:Female; D006085:Graft Survival; D006943:Hyperglycemia / Q000628:therapy*; D007108:Immune Tolerance; D007328:Insulin / Q000032:analysis / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000032:analysis / Q000276:immunology; D016381:Islets of Langerhans Transplantation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000601:surgery; D013997:Time Factors
D003470:Culture Media; D007328:Insulin