Thiourea prevents cytotoxicity and mutagenicity, but not sister-chromated exchanges in V79 cells treated with cis-diaminedichloroplatinum (II).
This work shows that cis-diaminedichloroplatinum(II)'s (cis-DDP) mutagenicity, as well as its cytotoxicity, can be prevented by subsequent treatment with thiourea. Sister-chromatid exchanges (SCE) induced by cis-DDP are only partially prevented by thiourea under conditions where mutagenicity is totally inhibited. A 4.5-h delay in the addition of thiourea following cis-DDP treatment results in an almost complete loss of its ability to prevent mutagenicity, but only a partial loss of its ability to prevent cytotoxicity and sister-chromatid exchanges.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D006224:Cricetinae; D003412:Cricetulus; D003434:Crossing Over, Genetic / Q000187:drug effects*; D009152:Mutagenicity Tests; D009153:Mutagens; D009154:Mutation; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013329:Structure-Activity Relationship; D013890:Thiourea / Q000494:pharmacology*
D009153:Mutagens; C034697:transplatin; D013890:Thiourea; D002945:Cisplatin