Rapid determination of hidden rheumatoid factor.
A method for the determination of hidden 19S IgM rheumatoid factor based on separation by QAE-Sephadex A-50 chromatography followed by hemolytic assay is described. Hidden rheumatoid factor titers and IgM recoveries of sera were comparable to those obtained by the conventional acid gel filtration method. The QAE method saves time, requires only 250 microliters of serum and can be performed with a minimum of specialized equipment.
D001171:Arthritis, Juvenile / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis; D012217:Rheumatoid Factor / Q000032:analysis*
D007075:Immunoglobulin M; D012217:Rheumatoid Factor