[Dependence of ganglioside composition of leukemic lymphocytes on density cell population density].
A comparative study of gangliosides from bovine normal lymph lymphocytes and leukemic blood lymphocytes and from calf thymocytes incubated at different cell densities was carried out. The ganglioside composition of thymocytes and normal lymphocytes was insensitive to the variations in cell density. In the case of leukemic lymphocytes the decrease of cell density resulted in a reduction of the absolute and relative content of one of the major ganglioside components, disialosyllactosylceramide, and in a concomitant increase of its precursor, N-glycoloylneuraminosyllactosylceramide. The content of the third major ganglioside component, sialosylparagloboside, did not depend on cell density. The cell density dependent changes in the ganglioside composition of leukemic lymphocytes are qualitatively coincident with those observed at variable concentrations of blood lymphocytes in leukemic animals. A possible mechanism underlying the differences in ganglioside metabolism during cell density changes in leukemic lymphocytes is discussed.
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000503:physiopathology*; D005732:Gangliosides / Q000502:physiology*; D007700:Kinetics; D007938:Leukemia / Q000503:physiopathology / Q000662:veterinary*; D008214:Lymphocytes / Q000502:physiology*
D005732:Gangliosides
Zavisimost' gangliozidnogo sostava leĭkemicheskikh limfotsitov ot plotnosti kletochnoĭ populiatsii.