title: Endocytotic uptake of cationized ferritin tracer into glomus cells dissociated from the adult rat carotid body.
abstract: Glomus cells from carotid bodies of adult rats dissociated by means of collagenase or collagenase + trypsin were used to study by electron microscopy the endocytotic uptake of cationized ferritin (CF) tracer into subcellular compartments. The glomus cells were incubated with the tracer (1) in a basic salt medium (BM), or (2) in the BM into which calcium ionophore A23187 had been added, or (3) in a potassium-rich medium. Incubation of the cells in BM containing CF for 30 min resulted in attachment of the tracer to the cell membrane and uptake of a few solitary tracer particles into small vesicles and multivesicular bodies. No uptake into the cisternae of the Golgi apparatus was observed. Further incubation in BM containing CF for another 30 min resulted in increased uptake of the tracer into small vesicles and multivesicular bodies. A similar pattern of uptake was observed when the dissociated glomus cells were first preincubated in BM with CF for 30 min and then incubated for 1 min or 30 min in the BM solution containing both the ionophore and CF. Upon such incubation, CF particles were seen to penetrate into coated pits and sites of exocytosis at the cell surface. When the 30-min preincubation in BM was followed by incubation in a CF-containing potassium-rich medium for 15-30 min, uptake into vesicles, small lysosomes and occasionally also into profiles of the smooth endoplasmic reticulum was seen. Endocytotic mechanisms of the glomus cells are outlined.
mesh_terms: D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002344:Carotid Body / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D003034:Coated Pits, Cell-Membrane / Q000648:ultrastructure; D004705:Endocytosis / Q000187:drug effects; D005260:Female; D005293:Ferritins / Q000378:metabolism*; D006651:Histocytochemistry; D066298:In Vitro Techniques; D008297:Male; D008854:Microscopy, Electron; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000001:Calcimycin; D005293:Ferritins; D011188:Potassium