Hemolytic activity of Leptospira interrogans serovar canicola cultured in protein-free medium.
Hemolytic activity of the culture supernatant of Leptospira interrogans serovar canicola strain Moulton grown in protein-free medium was demonstrated. The activity began to appear in the late logarithmic phase of growth of the organism and reached a plateau after 2 weeks of cultivation. It was inactivated by the addition of dipotassium ethylenediaminetetraacetate, but was effectively restored by Mg2+. Hemolysis by the culture supernatant was stimulated by "hot-cold" incubation. Sleep erythrocytes treated with the culture supernatant of the organism were transformed into spherocytes, in which invagination was observed. A hemolysis inhibitor in rabbit serum was found to be in the chloroform-methanol soluble fraction of the serum. The hemolysin of Leptospira may be phospholipase C.
D000818:Animals; D003470:Culture Media; D004492:Edetic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000648:ultrastructure; D006460:Hemolysin Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000494:pharmacology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007921:Leptospira interrogans / Q000254:growth & development*; D011817:Rabbits; D012756:Sheep
D003470:Culture Media; D006460:Hemolysin Proteins; D004492:Edetic Acid