The effect of native and single stranded DNA on the platelet release reaction. Enhancement of aggregated IgG-induced serotonin release.
Native (n) but not single stranded (ss) DNA was found to induce release of 3H-serotonin (5-HT) from platelets of the majority of normal individuals. However, ssDNA markedly enhanced 5-HT release induced by heat-aggregated IgG (aggIgG), while less enhancement was seen using nDNA. Similar enhancement was produced by polyinosinic acid but not by polyinosinic:polycytidylic acid. The ability of ssDNA to potentiate aggIgG-induced 5-HT release seemed specific for aggIgG, since no effect on ADP or epinephrine-induced release was observed and thrombin-induced release was inhibited. In contrast, nDNA in high concentrations (100 micrograms/ml) potentiated ADP, epinephrine, and thrombin-induced 5-HT release. These results suggest that ss-and nDNA may interact with platelets by different mechanisms and provide a means by which DNA, released at sites of tissue injury, could modulate the role of platelets in the inflammatory response. The ability of DNA to enhance the aggIgG-induced platelet release reaction may be important in immune complex diseases such as systemic lupus erythematosus.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism*; D004247:DNA / Q000494:pharmacology*; D004277:DNA, Single-Stranded / Q000494:pharmacology*; D004837:Epinephrine / Q000494:pharmacology; D006801:Humans; D007099:Imipramine / Q000494:pharmacology; D007074:Immunoglobulin G / Q000502:physiology; D011069:Poly I / Q000494:pharmacology; D011070:Poly I-C / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism*; D013917:Thrombin / Q000494:pharmacology
D004277:DNA, Single-Stranded; D007074:Immunoglobulin G; D011069:Poly I; D012701:Serotonin; D000244:Adenosine Diphosphate; D004247:DNA; D013917:Thrombin; D011070:Poly I-C; D007099:Imipramine; D004837:Epinephrine