Structure of DNA in metaphase chromosomes of mouse fibroblasts.
We show that N-1 in adenine of chromosomal DNA is methylated by treatment of metaphase chromosomes with dimethylsulphate while this is not the case in chromatin. The data on methylation are consistent with those obtained from the experiments with S1-nuclease treatment of chromatin and chromosomes. This suggests a disarrangement of DNA secondary structure in the metaphase chromosomes.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002875:Chromosomes / Q000032:analysis*; D004247:DNA / Q000032:analysis*; D005347:Fibroblasts / Q000032:analysis; D008677:Metaphase; D008745:Methylation; D051379:Mice
D004247:DNA