Influence of paracetamol (acetaminophen) on cadmium-induced lipid peroxidation in hepatocytes from starved rats.
Isolated hepatocytes form thiobarbituric acid (TBA)-reactants, a parameter of lipid peroxidation (LPO), when exposed to cadmium (Cd). Paracetamol (Para) inhibits this response, dependent on time and concentration. Cd-induced SH-group decline, however, and enhanced plasma membrane permeability to Trypan Blue (TB) were not inhibited by Para. This indicates that Cd-induced cell damage and Cd-induced LPO are independent events. Experiments using cells with enhanced and lowered metabolism of Para after phenobarbital- and acetone- or heat-and metyrapone treatment, respectively, suggest a potentially inhibitory effect of both Para and its metabolite(s) on LPO. The antioxidative potency of Para turned out to be small compared to that of the radical scavenger (+)-cyanidanol-3.
D000082:Acetaminophen / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D005260:Female; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013217:Starvation / Q000378:metabolism
D008054:Lipid Peroxides; D002104:Cadmium; D000082:Acetaminophen