Comparison of DMSO and glycerol as cryoprotectants for ascites tumor cells.
Mouse Ehrlich ascites and rat D23 ascites tumors were stored in liquid nitrogen under identical conditions for up to 3 years. Cell viability (trypan blue exclusion) and transplantability of both tumors in animals remained virtually unaffected if preserved in 10% DMSO containing medium, whereas, cells preserved in 10% glycerol failed to produce lethal tumors in rodents.
D000818:Animals; D001201:Ascites; D002286:Carcinoma, Ehrlich Tumor / Q000503:physiopathology; D002470:Cell Survival; D004121:Dimethyl Sulfoxide; D005615:Freezing; D005990:Glycerol; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000503:physiopathology*; D051381:Rats; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*
D005990:Glycerol; D004121:Dimethyl Sulfoxide