Increased concentration of thymidine kinase in rat hepatomas.
Thymidine kinase was purified to near homogeneity by affinity chromatography from cytosol fraction of rat hepatoma 3924A. The enzyme had a Mr of 81,000 and was composed of two subunits of Mr = 44,000. Antiserum made against it neutralized the activities of thymidine kinase from both rat livers and hepatomas. Neutralization studies with the antiserum revealed that hepatic transformation resulted in 4-, 15- and 25-fold increase in the amount of cytosol thymidine kinase in hepatomas 16, 7787 and 3924A of slow, medium and fast growth rate, respectively.
D000818:Animals; D002846:Chromatography, Affinity; D008099:Liver / Q000201:enzymology; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D046911:Macromolecular Substances; D008297:Male; D008970:Molecular Weight; D051381:Rats; D011915:Rats, Inbred BUF; D013937:Thymidine Kinase / Q000032:analysis / Q000378:metabolism*
D046911:Macromolecular Substances; D013937:Thymidine Kinase