Isolation and characterization of a lectin from garden cress (Lepidium sativuum).
A lectin has been isolated from extracts of garden cress (Lepidium sativum) by affinity chromatography on human immunoglobulin-Sepharose. The lectin reacts with human erythrocytes without specificity for the A, B and 0 blood group. Erythrocytes of animal origin are also agglutinated by the lectin. The hemagglutinating activity is abolished by heating the lectin solution at 70 degrees C or by dialysis against strong acid buffers. The hemagglutination reaction is not inhibited by monosaccharides. Lectin-glycoprotein interactions are described and discussed.
D000017:ABO Blood-Group System; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D008970:Molecular Weight; D037121:Plant Lectins; D012639:Seeds / Q000032:analysis*; D012794:Sialic Acids / Q000494:pharmacology
D000017:ABO Blood-Group System; D037102:Lectins; D037121:Plant Lectins; D012794:Sialic Acids