Immunological properties of rat lens gamma-crystallins. I. Characterization of the major components.
Rat lens gamma-crystallins were separated by preparative flat bed isoelectric focusing (pH 7-11). The fractions obtained were submitted to analytical isoelectric focusing, fused rocket immunoelectrophoresis and immunodiffusion. The results demonstrate that rat lens gamma-crystallins are electrophoretically and immunologically heterogenous. Evidence is given that there are two immunologically different groups. Rat gamma 1-crystallin, the prominent and immunologically homogeneous part, is identical to calf lens gamma-crystallin. The second group of proteins, gamma 2-crystallins, is heterogeneous and shows only partial identity with gamma 1-crystallin.
D000818:Animals; D002417:Cattle; D003459:Crystallins / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing; D007908:Lens, Crystalline / Q000032:analysis / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains
D003459:Crystallins