Isolation and characterization of colipase from porcine and human pancreatic juice by immunoaffinity chromatography.
Pure colipase was prepared by immunoaffinity chromatography from porcine and human pancreatic juice. A single form of the porcine colipase was obtained, having the structural and biological properties of previously characterized porcine procolipase A. Two forms of activated colipase (N-terminal Gly) were isolated from human pancreatic juice by the same procedure. The existence of two forms of activated colipase might arise from rapid activation of a precursor form of human colipase during collection of the pancreatic juice.
D000818:Animals; D002846:Chromatography, Affinity; D003089:Colipases / Q000302:isolation & purification*; D006801:Humans; D010189:Pancreatic Juice / Q000032:analysis*; D011506:Proteins / Q000302:isolation & purification*; D013552:Swine
D003089:Colipases; D011506:Proteins