The character of growth of mycobacterium tuberculosis in vitro in dependence on peripheral human blood's components.
Blood plasma, cell mass, white blood cells (WBC) and erythrocytes were investigated for their relation to cord formation of M. tuberculosis, cultivated in blood according to Pryce's method. It was found that cord formation was related to blood cell and followed the zone of sedimentation of WBC. In lysed blood cord formation could be revealed on the whole glass surface, but disappeared if the lysed blood was filtered through Seitz filter - evidences which were accepted as indications that cord formation was dependent on WBC. However, destroying WBC in lysed blood by freezing and thawing or eliminating them by centrifugation did not disturb cord formation and addition of WBC to simple media did not result in cord formation. It was concluded that cord formation was called forth by lysed erythrocytes and this was confirmed through adding erythrocytes stromata to simple media, where cord formation appeared. The practical value of the technique applied and the eventual role of erythrocyte stromata's lipids in cord formation are discussed.
D003470:Culture Media; D004912:Erythrocytes / Q000502:physiology*; D006461:Hemolysis; D007962:Leukocytes / Q000502:physiology; D009169:Mycobacterium tuberculosis / Q000166:cytology / Q000254:growth & development*
D003470:Culture Media