Resistance of A-431 epidermoid carcinoma cells to early membrane changes induced by tumour promoters.
The A-431 human epidermoid carcinoma cell line was resistant to 12-O-tetradecanoylphorbol-13-acetate (TPA) inhibition of epidermal growth factor binding, enhanced incorporation of [3H]choline into phospholipids and uptake of 86Rb an [3H]2-deoxyglucose. The cells were also resistant to TPA-stimulated release of radioactive choline derivatives and arachidonic acid from cells prelabelled with [3H]choline or [14C]arachidonic acid, respectively. The A-431 cells did not metabolise [3H]TPA. Despite their TPA-unresponsiveness, A-431 cells contained specific, high affinity binding sites for [3H]phorbol-12,13-dibutyrate with characteristics similar to other cultured cell lines.
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002273:Carcinogens / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002478:Cells, Cultured; D002794:Choline / Q000378:metabolism; D004351:Drug Resistance; D006801:Humans; D008566:Membranes / Q000187:drug effects / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D001095:Arachidonic Acids; D002273:Carcinogens; D010743:Phospholipids; D016718:Arachidonic Acid; D002794:Choline; D013755:Tetradecanoylphorbol Acetate