Studies of terminal deoxynucleotidyl transferase in normal and neoplastic human cells.
Optimized biochemical assays and cytoimmunofluorescence tests were used to detect terminal deoxynucleotidyl transferase, TdT, in malignant cells of 36 leukemias and 75 lymphomas from patients not receiving chemotherapy. TdT was virtually absent from normal lymph nodes and from leukocytes of chronic lymphocytic leukemia, CLL, taken as controls. Its quantitative distribution in the neoplasms matched the current knowledge. Appreciable amounts of TdT were found in all the 10 lymphomas of lymphoblastic type, LL, and in the white blood cells of: 16 out of 19 acute lymphoblastic leukemia, AAL, perhaps with modulation in the various phenotypes; 2 out of 3 acute undifferentiated leukemias, AUL; and 3 out of 7 blastic crises in chronic myelogenous leukemia, b.c. CML. Biochemical and cytoimmunological analyses yielded concordant responses and even roughly comparable estimates in the same patients. TdT immunofluorescence was clearly nuclear in most cells and was cytoplasmic occasionally. Definite correlations between concentrations of enzymatic activity and percentage of immunofluorescent cells could not e established. Further detailed work will be required to identify putative subgroups in TdT-positive blast populations.
D000818:Animals; D000936:Antigen-Antibody Complex; D002417:Cattle; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006689:Hodgkin Disease / Q000201:enzymology; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G; D007938:Leukemia / Q000201:enzymology*; D008223:Lymphoma / Q000201:enzymology*; D012016:Reference Values; D013945:Thymoma / Q000201:enzymology*; D013950:Thymus Gland / Q000201:enzymology*; D013953:Thymus Neoplasms / Q000201:enzymology*
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D004254:DNA Nucleotidyltransferases; D004253:DNA Nucleotidylexotransferase