Establishment of nucleolar deficient sublines of PtK2 (Potorous tridactylis) by ultraviolet laser microirradiation.
One of the two nucleoli of tetraploid PtK2 WA cells in early prophase was irradiated with an ultraviolet (UV) laser microbeam. The daughter cells that maintained the nucleolar deficiency were isolated and cloned. Five nucleolar deficient sublines of PtK2 WA were established, thus providing an experimental system to study the ribosomal gene-nucleolar organizer complex.
D000818:Animals; D002460:Cell Line; D002466:Cell Nucleolus / Q000502:physiology*; D002999:Clone Cells; D007834:Lasers; D007614:Macropodidae; D008297:Male; D008394:Marsupialia; D009697:Nucleolus Organizer Region / Q000528:radiation effects; D014466:Ultraviolet Rays