The synthesis of the brain specific S100 protein in colcemid resistant mutants of rat glial cells.
We have isolated two colcemid-resistant mutant sublines, CMR (7A) and CMR (7B), from rat glial cells, C6, using multiple consecutive selections with increasing concentrations of colcemid. The mutant sublines show a decreased uptake of [3H]colchicine but have no apparent defect in the cytoplasmic binding of the drug. The synthesis of the brain-specific S100 protein is less sensitive to colcemid inhibition in the mutant cell lines than in parental C6 cells, suggesting that colcemid must enter the cell to inhibit S100 protein synthesis.
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D001923:Brain Chemistry; D002460:Cell Line; D003078:Colchicine / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D003703:Demecolcine / Q000378:metabolism / Q000494:pharmacology*; D004351:Drug Resistance; D008870:Microtubules / Q000187:drug effects*; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009457:Neuroglia / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D009418:S100 Proteins / Q000096:biosynthesis*
D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D009418:S100 Proteins; D003078:Colchicine; D003703:Demecolcine