Non-centrifugal plasma collection using cross-flow membrane plasmapheresis.
A prototype of a cross-flow membrane plasmapheresis system was tested for its safety and efficacy in normal donors. 550 ml of plasma can be harvested in less than 1 h. The procedure appears to be safe for the donor. No significant alterations in donor hematological, coagulation, or biochemical values were obtained after the procedure compared to the predonation levels. Changes in the classical and alternate complement pathways were studied serially by analysis of functional hemolytic titers, C3 and C4 levels, and C3 and factor B cleavage products. No firm evidence of complement activation by the polysulfone filtration cell membrane was found.
D000328:Adult; D001772:Blood Cell Count; D001779:Blood Coagulation Factors / Q000032:analysis; D001800:Blood Specimen Collection; D002498:Centrifugation; D003167:Complement Activation; D005260:Female; D005374:Filtration; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D010949:Plasma / Q000032:analysis; D010956:Plasmapheresis / Q000295:instrumentation* / Q000379:methods
D001779:Blood Coagulation Factors; D006454:Hemoglobins; D008567:Membranes, Artificial