[A new method for human milk protein separation].
Attempts at isolating individual human milk proteins showed that cross interactions made it difficult to obtain of homogeneous components. A new method was devised, based on complete precipitation of milk proteins with saturated ammonium sulphate and progressive solubilization of the precipitate on a column of Sephadex G10 with a linear gradient of ammonium sulphate (from saturation to water). Three fractions were obtained. The first contained lactoferrin, serum albumin, lysozyme and traces of alpha-lactalbumin. Lysozyme could be obtained free from contaminants by chromatography on Ultrogel AcA 54. Lactoferrin and serum albumin coeluting as a single peak, were separated by a further chromatography on DEAE-cellulose. From the other two fractions recovered on Sephadex G10, it should be possible to prepare immunoglobulins, alpha-lactalbumin and the bulk of caseins. The homogeneity of the preparations of lysozyme, lactoferrin and serum albumin was assessed by SDS polyacrylamide gel electrophoresis, acrylamide agarose electrophoresis and immunoelectrophoresis.
D002850:Chromatography, Gel; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D007768:Lactalbumin / Q000302:isolation & purification; D007781:Lactoferrin / Q000302:isolation & purification; D008894:Milk Proteins / Q000302:isolation & purification*; D008895:Milk, Human / Q000032:analysis*; D009113:Muramidase / Q000302:isolation & purification; D012709:Serum Albumin / Q000302:isolation & purification
D008894:Milk Proteins; D012709:Serum Albumin; D007768:Lactalbumin; D009113:Muramidase; D007781:Lactoferrin
A new method for human milk protein separation.