Glutamate decarboxylase immunoreactivity and gamma-[3H] aminobutyric acid accumulation within the same neurons in dissociated cell cultures of cerebral cortex.
In order to evaluate the reliability of high affinity [3H]GABA accumulation as a marker for GABAergic neurons, murine cerebral cortical neurons were studied in dissociated cell culture. Cultures which had been incubated in [3H]GABA were stained immunohistochemically for the GABA-synthesizing enzyme, glutamate decarboxylase, fixed with paraformaldehyde, and subsequently processed for radioautography. In mature cultures, there was an 84 to 94% correlation between the presence of the enzyme and [3H]GABA uptake within the same cortical neurons. These data provide direct evidence that those neurons which synthesize GABA are the same neurons which are labeled by high affinity [3H]GABA uptake.
D000818:Animals; D001345:Autoradiography; D001692:Biological Transport; D002262:Carboxy-Lyases / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism*; D005333:Fetus; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D006651:Histocytochemistry; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism; D014316:Tritium; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D014316:Tritium; D005680:gamma-Aminobutyric Acid; D002262:Carboxy-Lyases; D005968:Glutamate Decarboxylase