title: Effects of dietary choline deficiency on the mutagenic activation of chemical carcinogens by rat liver fractions.
abstract: Salmonella mutagenesis assays were used to evaluate the mutagenicity of several chemical carcinogens as mediated by liver S-9 fractions from rats fed a choline-supplemented (CS) or choline-devoid (CD) diet. The liver S-9 fraction from CD diet-fed rats was found to have a significantly decreased ability to activate 2-acetylaminofluorene (2-AAF), 2-aminoanthracene (2-AA) and 6-aminochrysene (6-AC), but not N-hydroxy-2-acetylaminofluorene (HO-N-2-AAF) and dimethylnitrosamine (DMN). The same liver S-9 fraction was also less effective in deactivating N-methyl-N1-nitro-N-nitrosoguanidine (MNNG) but not methylnitrosourea (MNU). A decrease (20%) in the cytochrome P-450 content was found in liver microsomes of CD diet-fed rats. Although it has been shown that feeding a CD diet to rats enhances chemical hepatocarcinogenesis, the data presented here suggest that CD diet does not increase the activation of the chemical procarcinogens tested.
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000378:metabolism*; D002796:Choline Deficiency / Q000378:metabolism*; D003043:Cocarcinogenesis; D004032:Diet; D008099:Liver / Q000378:metabolism*; D008113:Liver Neoplasms / Q000139:chemically induced; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens; D009374:Neoplasms, Experimental / Q000139:chemically induced; D051381:Rats; D012475:Salmonella / Q000187:drug effects
chemical_list: D002273:Carcinogens; D009153:Mutagens