The effects of colchicine on the distribution of glycoprotein-containing vesicles in epithelial cells of the murine colon.
In murine colonic epithelial cells, cell-coat glycoproteins are transported to the cell surface in vesicles that originate at the Golgi apparatus. To determine the role of microtubules in the movement of these vesicles the antimicrotubule agent colchicine was injected into mice at several time intervals prior to sacrifice. In the mice that were treated with colchicine for 4.5 h it was observed that the polarity of the cells was disturbed. The Golgi apparatus and nucleus often appeared interchanged in their positions. The glycoprotein-containing vesicles, normally located apically, were sparse in that location, but abundant near the lateral plasma membranes of the cells at the level of the nucleus and Golgi apparatus. Straining by the periodic acid-chromic acid-silver methenamine technique for glycoproteins clearly revealed the reduction of vesicles apically and accumulation of vesicles laterally. The mechanism responsible for the movement of the vesicles to this location is unclear. It is suggested that the accumulation of vesicles in the lateral region may reflect some hindrance in the fusion of the vesicles with the lateral cell membranes.
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D003078:Colchicine / Q000494:pharmacology*; D003106:Colon / Q000187:drug effects / Q000648:ultrastructure*; D003594:Cytoplasmic Granules / Q000032:analysis / Q000187:drug effects / Q000502:physiology*; D004848:Epithelium / Q000648:ultrastructure; D006023:Glycoproteins / Q000032:analysis*; D006056:Golgi Apparatus / Q000648:ultrastructure; D006651:Histocytochemistry; D008297:Male; D051379:Mice
D006023:Glycoproteins; D003078:Colchicine