[Enzymatic release of sedimentary bacteria in the presence of antibiotics].
Polysaccharases release microorganisms from their natural seat, marine sediments for example. The enzymatic activity works both on the microbial adherence polysaccharides and on the support surfaces (cellulose, pectine, etc.). Dosages of glucose confirm polysaccharase activity. An association of bacitracine, thiophenicol and a few enzymes: cellulase, pectinase, amyloglucosidase, alpha amylase, hyaluronidase, release a considerable number of bacteria. The culture on specific mediums confirm the specificity of this release. E. coli polyresistant strain where isolated by amylo-glucosidase, glucuronidase association in a mixture of thiophenicol and bacitracine. Bacillus and other Gram positive bacteria are frequently isolated by this method. The number of colonizer microorganisms on solid media are considerably higher with sediments treated by enzymes, or by enzyme, antibiotic mixtures, than with untreated ones.
D001414:Bacitracin / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002480:Cellulase / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology; D005087:Glucan 1,4-alpha-Glucosidase / Q000494:pharmacology; D005966:Glucuronidase / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000494:pharmacology*; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D011096:Polygalacturonase / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000502:physiology; D012623:Seawater; D013839:Thiamphenicol / Q000494:pharmacology*; D000516:alpha-Amylases / Q000494:pharmacology
D011135:Polysaccharides, Bacterial; D001414:Bacitracin; D006026:Glycoside Hydrolases; D000516:alpha-Amylases; D011096:Polygalacturonase; D005087:Glucan 1,4-alpha-Glucosidase; D005966:Glucuronidase; D006821:Hyaluronoglucosaminidase; D002480:Cellulase; D013839:Thiamphenicol
Débusquement enzymatique des bactéries sédimentaires en présence d'antibiotiques.