Glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides. Isolation and sequence of a peptide containing an essential lysine.
Interaction of glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides with pyridoxal 5'-phosphate and sodium borohydride leads to inactivation and modification of two lysine residues per enzyme dimer that are thought to bind glucose 6-phosphate [Milhausen, M., & Levy, H.R. (1975) Eur. J. Biochem. 50, 453-461]. The amino acid sequence surrounding this lysine residue is reported. Following tryptic hydrolysis of the modified enzyme, two peptides, each containing one pyridoxyllysine residue, were purified to homogeneity and subjected to automated Edman degradation. The sequences revealed that one of these, a heptapeptide, was derived from the other, containing 11 amino acids. Supporting evidence for the role of the modified lysine is provided in the following paper [Haghighi, B., & Levy, H.R. (1982) Biochemistry (second paper of three in this issue)]. End-group analysis of the native enzyme revealed that valine is the N-terminal and glycine the C-terminal amino acid and provides support for the identity of the enzyme's two subunits.
D000595:Amino Acid Sequence; D001894:Borohydrides / Q000494:pharmacology; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis / Q000302:isolation & purification*; D007934:Leuconostoc / Q000201:enzymology*; D008239:Lysine / Q000032:analysis; D046911:Macromolecular Substances; D010446:Peptide Fragments / Q000032:analysis; D011732:Pyridoxal Phosphate / Q000494:pharmacology; D014357:Trypsin / Q000378:metabolism
D001894:Borohydrides; D046911:Macromolecular Substances; D010446:Peptide Fragments; D011732:Pyridoxal Phosphate; D005954:Glucosephosphate Dehydrogenase; D014357:Trypsin; D008239:Lysine