Origin and production rates of deoxycorticosterone and deoxycorticosterone sulfate in men and nonpregnant women.
In the present investigation, we evaluated the origin of deoxycorticosterone sulfate (DOC-SO4) and the production rates of DOC and DOC-SO4 in men and women. Previously, we found that there was little or no interconverion of plasma DOC and DOC-SO4; this finding was reconfirmed in the present investigation. After the iv infusion of [3H]DOC-SO4 and [14C]DOC, urine was collected for 5 days, DOC-SO4 was isolated and purified as unconjugated DOC, and tetrahydro-DOC glucuronoside was isolated and purified as the unconjugated metabolite. The production rate of DOC in these subjects (mean +/- SEM, 66 +/- 9.8 micrograms/24 h) was computed from the specific activity of urinary [14C]tetrahydro-DOC (glucuronoside); the production rate of DOC-SO4 in these subjects (92 +/- 15.9 micrograms/24 h) was computed from the specific activity of urinary [3H]DOC-SO4. The production rates are expressed on the basis of the molecular weight of DOC. Since plasma DOC and DOC-SO4 are not interconverted, we conclude that both steroids are secretory products, presumably from the adrenal cortex.
D000328:Adult; D002250:Carbon Radioisotopes; D003900:Desoxycorticosterone / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000652:urine; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; C032268:deoxycorticosterone sulfate; D003900:Desoxycorticosterone; C009413:tetrahydrodeoxycorticosterone