[Demonstration of the pulsatile secretion of LH-RH into hypophysial portal blood of ewes using an original technic for multiple samples].
Using a stereotaxic dorsal approach under lateral X-Ray control associated with a perfusion system, serial collections of hypophysial portal blood have been successfully obtained in three spayed ewes during 4 to 9 successive hrs. For the first time, pulsatile patterns of LH-RH secretion have been demonstrated in long term ovariectomized ewes. The frequency of LH-RH pulses recorded (about one pulse every 50 mn) agrees closely with the frequency of LH pulses in peripheral plasma of ovariectomized ewes. The technique allowed measurement of LH-RH in hypophysial portal blood thus providing a useful method for investigation of hypothalamo-pituitary gonadal interactions.
D000818:Animals; D002369:Castration; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000097:blood / Q000378:metabolism*; D010902:Pituitary Gland / Q000098:blood supply / Q000378:metabolism*; D011168:Portal System; D012756:Sheep; D013997:Time Factors
D007987:Gonadotropin-Releasing Hormone
Mise en évidence d'une sécrétion pulsatile du LH-RH du sang porte hypophysaire chez la brebis par une technique originale de prélvements multiples.