DNA-flow-cytometric measurements on the normal, atrophic, hyperplastic and neoplastic human endometrium.
DNA distribution patterns and the fractions of the cell cycle phases were determined by means of flow-through cytometry in 87 samples of normal, atrophic, hyperplastic and carcinomatous human endometrium. The S-phase fractions vary during the normal menstrual cycle between 1 and 3% and reach a periovulatory maximum between 4.4 and 4.7%. Atrophic endometrium and regressive glandular cystic hyperplasia have little DNA synthesis (1.01% and 1.68% S-phase fractions respectively). Proliferating glandular cystic hyperplasia reveals 3.38% S-phase fraction, whereas adenomatous hyperplasia has an increased number of DNA-synthesizing cells (4.81%). The well-differentiated endometrial carcinoma shows no cytophotometrically detectable differences in comparison to adenomatous hyperplasia. All endometrial samples except for poorly differentiated endometrial carcinoma showed a diploid to tetraploid DNA distribution pattern. The poorly differentiated endometrial carcinoma displays two different types: one rapidly growing diploid-tetraploid tumor with 8.0 to 9.6% S-phase fractions, and another type with stemline deviations, polyploid nuclei and less pronounced synthetic activity.
D000236:Adenoma / Q000473:pathology; D001284:Atrophy; D002453:Cell Cycle; D003560:Cysts / Q000473:pathology; D004247:DNA / Q000096:biosynthesis; D004714:Endometrial Hyperplasia / Q000473:pathology*; D004717:Endometrium / Q000166:cytology* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008598:Menstruation; D014594:Uterine Neoplasms / Q000473:pathology*
D004247:DNA