Effect of some vasodilating and antihypertensive drugs on the in vitro biosynthesis of prostaglandins from (1-14C) arachidonic acid in washed human blood platelets.
Effects of some vasodilating (oxyfedrine, dipyridamole, verapamil and nifedipine) and antihypertensive (propranolol, hydralazine, prazosin and dihydralazine) drugs on the human blood platelet biosynthesis of prostaglandins from labelled arachidonic acid have been examined in vitro. Malondialdehyde (MDA) production in platelets and their aggregation in presence of these drugs were also studied. All the drugs inhibited the generation of thromboxane A2 which correlated well with the reduced platelet formation of MDA in presence of several of these drugs. In case of seven of the eight drugs, separation of HHT from HETE could be made. Values of these metabolites - especially those of HHT were found to be reduced, thus supporting the MDA and TxA2 data. With several of these drugs, ADP-induced platelet aggregation was inhibited either completely or partially. The results show that the well-known completely or partially. The results show that the well-known effects of these drugs might well be augmented by their effect on the platelet metabolism of arachidonic acid.
D000959:Antihypertensive Agents / Q000494:pharmacology*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D010974:Platelet Aggregation / Q000187:drug effects; D011451:Prostaglandin-Endoperoxide Synthases / Q000097:blood; D011453:Prostaglandins / Q000096:biosynthesis*; D013930:Thromboxane-A Synthase / Q000097:blood; D014665:Vasodilator Agents / Q000494:pharmacology*
D000959:Antihypertensive Agents; D001095:Arachidonic Acids; D011453:Prostaglandins; D014665:Vasodilator Agents; D016718:Arachidonic Acid; D011451:Prostaglandin-Endoperoxide Synthases; D013930:Thromboxane-A Synthase