Conversion of 3H-PAF acether by rabbit platelets is independent from aggregation: evidences for a novel metabolite.
3H-PAF-acether (Alkyl-[1', 2'-3H]- 2-acetyl-sn-glyceryl-3-phosphorylcholine) was time-dependently transformed by plasma-free rabbit platelets into an unknown product, "PX", which was distinct from lyso-PAF-acether. This effect was specific for platelets since plasma only converted 3H-PAF-acether into lyso-3H-PAF-acether and platelets were not effective in metabolizing lyso-3H-PAF-acether. Platelet aggregation did not interfere with the formation of "PX". The latter is a novel platelet metabolite of PAF-acether with as yet unknown biological properties.
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D007700:Kinetics; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000097:blood; D010974:Platelet Aggregation; D011817:Rabbits; D014316:Tritium
D010972:Platelet Activating Factor; D014316:Tritium; C026613:1-0-octadecyl 2-0-acetyl sn-glycero-3-phosphorylcholine