title: Decrease of E and EA but not EAC rosette formation after incubation of blood lymphocytes in cerebrospinal fluid.
abstract: Peripheral blood lymphocytes (PBL) were incubated in autologous cerebrospinal fluid (CSF) or Hanks' balanced salt solution (HBSS) for between 5 and 60 min at 37 degrees C. Incubation in CSF caused an increase in cell death as measured by trypan blue inclusion. Incubation in CSF also resulted in a decreased percentage of E and EA rosette-forming cells (RFC)--but not of EAC-RFC--as compared with preincubation levels. Incubation in HBSS did not cause significant changes in rosette formation. Although incubation in CSF resulted in decreased E and EA rosette formation by PBL, lymphocytes isolated from CSF showed increased E and EAC rosette formation as compared with PBL.
mesh_terms: D002470:Cell Survival; D002555:Cerebrospinal Fluid / Q000166:cytology* / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000145:classification / Q000378:metabolism*; D011946:Receptors, Antigen / Q000032:analysis*; D011951:Receptors, Complement; D012397:Rosette Formation; D013997:Time Factors
chemical_list: D011946:Receptors, Antigen; D011951:Receptors, Complement