title: Effects of methionine sulphoximine on protein transients during heterocyst differentiation in Anabaena and Cylindrospermum spp.
abstract: Heterocystous blue-green algae normally do not develop heterocysts if grown in the medium enriched with ammonium compounds. If such cultures are resuspended in a medium lacking combined nitrogen, the heterocysts differentiate within 24 h. L-methionine-DL-sulphoximine (MSO) has been reported to induce heterocysts even in the presence of ammonium chloride. The changes in gel electrophoresis protein profiles of cultures developing heterocysts, in the ammonium chloride enriched medium supplemented with MSO suggest that heterocyst differentiation in the presence of ammonium chloride and MSO may be due to derepression of heterocyst formation genes by MSO.
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D000458:Cyanobacteria / Q000187:drug effects / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D008717:Methionine Sulfoximine / Q000494:pharmacology*; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D011506:Proteins; D008717:Methionine Sulfoximine