A reassessment of the mechanism of action of 4'-[(9-acridinyl)-amino]methanesulphon-m-anisidide.
The conclusion that treating cells with 4'-[(9-acridinyl)- amino]methanesulphon-m-anisidide (mAMSA) causes an alkali-dependent decrease in the size of their DNA was reinvestigated. Evidence is presented that alkali is not needed to detect an effect of mAMSA on the sedimentation rate of DNA released from mAMSA-treated PY815 cells and that mAMSA causes double-strand breaks in cellular DNA.
D000609:Aminoacridines / Q000494:pharmacology*; D000677:Amsacrine; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D004273:DNA, Neoplasm / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D012515:Mast-Cell Sarcoma / Q000378:metabolism* / Q000473:pathology; D051379:Mice
D000609:Aminoacridines; D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D000677:Amsacrine