Localization of glial fibrillary acidic protein in retinal astrocytoma: an immunohistochemical study.
Glial fibrillary acidic protein (GFA) is found in glial filaments, and is specific for reactive or neoplastic astrocytes and ependyma. Using a commercially available antibody to GFA protein, a retinal neoplasm from a patient with neurofibromatosis is intensely stained with the brown immunoperoxidase reaction product. Rather than the less specific phosphotungstic acid hematoxylin (PTAH) or Holzer's stain, antibody to GFAP protein is a relatively rapid, simple, and reliable immunohistochemical technique for formalin-fixed and paraffin-embedded tissue.
D001254:Astrocytoma / Q000032:analysis* / Q000175:diagnosis; D002648:Child; D005134:Eye Neoplasms / Q000032:analysis* / Q000175:diagnosis; D005904:Glial Fibrillary Acidic Protein; D006801:Humans; D007124:Immunoenzyme Techniques; D007381:Intermediate Filament Proteins / Q000032:analysis*; D008297:Male; D009378:Neoplasms, Multiple Primary / Q000175:diagnosis; D009456:Neurofibromatosis 1 / Q000175:diagnosis*; D012164:Retinal Diseases / Q000175:diagnosis; D014062:Tongue Neoplasms / Q000175:diagnosis*
D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins