title: Changes in mitochondrial respiration during the development of Xenopus laevis.
abstract: Mitochondria isolated from Xenopus laevis embryos at various developmental stages show a good oxidative capacity and an acceptable respiratory control provided that certain requirements are fulfilled. The rates of respiration with pyruvate and Krebs' cycle intermediates, especially with citrate and isocitrate, are very low during cleavage stages and increase after gastrulation. Glutamate in the presence of malate is the only substrate to be readily oxidized during early development and its rate of oxidation decreases after gastrulation. These results, together with the altered sensitivity of embryonic mitochondria towards azide, support the view that the oxidative metabolism undergoes important changes around gastrulation and is associated with mitochondrial differentiation.
mesh_terms: D000367:Age Factors; D000818:Animals; D001386:Azides / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005775:Gastrula / Q000502:physiology; D005971:Glutamates / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D010710:Phosphates / Q000494:pharmacology; D011773:Pyruvates / Q000378:metabolism; D014982:Xenopus laevis / Q000196:embryology*
chemical_list: D001386:Azides; D005971:Glutamates; D010710:Phosphates; D011773:Pyruvates; D004492:Edetic Acid; D008274:Magnesium