Diazepam inhibits the spreading of chick embryo fibroblasts.
Diazepam (Valium/Roche), a benzodiazepine derivative, reversibly inhibited the spreading of chick embryo fibroblasts in a dose-related manner. This inhibition was accompanied by: 1 Fewer and less organized microfilament bundles. 2 A reduction in myosin fluorescence along these structures. 3 A reduction in the total myosin content of cells compared to corresponding controls. Overall results suggest that myosin plays an active role in the spreading of chick embryo fibroblasts.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002642:Chick Embryo; D003975:Diazepam / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D009218:Myosins / Q000378:metabolism; D014018:Tissue Distribution
D009218:Myosins; D003975:Diazepam