Reversed-phase thin layer chromatography of some common sterols.
The chromatographic mobilities of 17 sterols and squalene on reversed-phase thin layer plates with four nonaqueous solvent systems is described. A degree of separation adequate to identify several of the sterols was obtained. It was possible to separate the pairs: cholestanol, epicholestanol; coprostanol, epicoprostanol; 5 beta-cholestan-3 alpha and 3 beta-ol and lanosterol, dihydrolanosterol.
D002855:Chromatography, Thin Layer / Q000379:methods; D007202:Indicators and Reagents; D012997:Solvents; D013261:Sterols / Q000302:isolation & purification*; D013329:Structure-Activity Relationship
D007202:Indicators and Reagents; D012997:Solvents; D013261:Sterols