Resistance of established islet allografts to rejection by antibody and complement.
Using preliminary in vitro culture in an oxygen-rich atmosphere to facilitate survival of the graft, long term survival (greater than 100 days) of mouse pancreatic islet allografts was obtained subsequently in non-immunosuppressed diabetic animals. When anti-donor alloantiserum was transferred to these animals, the grafts were not rejected despite the fact that circulating cytotoxic antibody was demonstrated up to 7 days after injection of alloantiserum. Similarly, there was no graft rejection following the injection of anti-donor alloantiserum and complement. These results show that cultured islet allografts are not susceptible to antibody and complement.
D000818:Animals; D003165:Complement System Proteins / Q000502:physiology*; D046508:Culture Techniques; D006084:Graft Rejection; D007515:Islets of Langerhans / Q000276:immunology; D016381:Islets of Langerhans Transplantation; D007518:Isoantibodies / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D014184:Transplantation, Homologous
D007518:Isoantibodies; D003165:Complement System Proteins