title: The effect of stannous and stannic (tin) chloride on DNA in Chinese hamster ovary cells.
abstract: Tin(II) at concentrations up to 500 microM stannous chloride (SnCl2), produced extensive DNA damage, as detected by alkaline sucrose gradient (ASG) analysis in Chinese hamster ovary (CHO) cells treated for 1 h at 37 degrees C in serum-free minimal essential medium (MEM). However treatment of cells with tin(IV), as stannic chloride (SnCl4), produced no such DNA damage. There was no loss in colony formation 6 days after either treatment suggesting that the DNA damage induced by the tin(II) was rapidly repaired and/or that DNA synthesis proceeded on the damaged templates permitting cell division to occur. Alternatively, the type of DNA damage caused by tin(II) may not lead to a reduction in colony-forming ability. Tin(II) produced about 200 times more ASG detectable DNA damage on an equi-molar basis than did Cr(VI), a known human carcinogen. This study indicates that tin(II) may be potentially genotoxic.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D004247:DNA / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics; D005260:Female; D009690:Nucleic Acid Conformation / Q000187:drug effects; D010053:Ovary; D014001:Tin / Q000494:pharmacology*
chemical_list: D004277:DNA, Single-Stranded; D014001:Tin; D004247:DNA