Some age-related structural peculiarities of fractionated chromatin. Thermal denaturation and electrophoretic analysis.
Thermal denaturation and electrophoretic mobility of liver and small intestinal mucosa fractionated chromatin and the DNA of chromatin fractions have been studied in young and old rats. During aging, the thermostability of chromatin increased, electrophoretic mobility of mono-, di-, and trinucleosomal fragments decreased at the expense of increased repeated length of nucleosomal DNA.
D000375:Aging; D000818:Animals; D002843:Chromatin / Q000032:analysis / Q000648:ultrastructure*; D004247:DNA / Q000032:analysis; D003720:Densitometry; D004589:Electrophoresis, Disc; D005260:Female; D006358:Hot Temperature; D007413:Intestinal Mucosa / Q000648:ultrastructure; D008099:Liver / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
D002843:Chromatin; D004247:DNA