Human monocytes synthesize eicosanoids from T lymphocyte-derived arachidonic acid.
T lymphocytes prelabeled with [14C] arachidonic acid failed to synthesize any eicosanoids even following stimulation with phytohemagglutinin, but they did release free [14C] arachidonic acid. Co-culture of unlabeled monocytes with the prelabeled T lymphocytes resulted in the synthesis of [14C] thromboxane B2, a major monocyte-derived eicosanoid. These data show that monocytes can utilize T lymphocyte-derived arachidonic acid for the synthesis of eicosanoids.
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002478:Cells, Cultured; D015232:Dinoprostone; D006801:Humans; D008213:Lymphocyte Activation; D009000:Monocytes / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011458:Prostaglandins E / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000378:metabolism*; D013929:Thromboxane B2 / Q000096:biosynthesis*; D013931:Thromboxanes / Q000096:biosynthesis*
D001095:Arachidonic Acids; D010835:Phytohemagglutinins; D011458:Prostaglandins E; D013931:Thromboxanes; D016718:Arachidonic Acid; D013929:Thromboxane B2; D015232:Dinoprostone