[Characterization and standardization of allergen extracts].
The allergenic extracts employed in the diagnostic or therapeutic fields are prepared from the different raw materials employing different extraction fluids: it has been shown that the solute contains, besides the allergens, many other substances. The analytical techniques, of which some of the most useful are described, should be implemented for the characterization and standardization of the extracts. The results obtainable, when using these techniques, for the extract characterization, are presented. Subsequently, the most important allergens identified and purified are briefly reviewed. The use of the analytical techniques described allows verifying the changes occurring in the allergenic extracts during storage. The implementation of the same allows the evaluation of the possibility, in subsequent times, of preparing, therefore standardizing, replicated allergenic extracts, similar to the former insofar as the allergenic and antigenic characteristics are concerned.
D000485:Allergens / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification / Q000592:standards; D000906:Antibodies / Q000032:analysis; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007073:Immunoglobulin E / Q000032:analysis; D007525:Isoelectric Focusing; D010323:Passive Cutaneous Anaphylaxis; D011852:Radioallergosorbent Test; D012882:Skin Tests
D000485:Allergens; D000906:Antibodies; D007073:Immunoglobulin E
Caratterizzazione e standardizzazione degli estratti allergenici.