[Microdetermination of lead in hair samples by graphite-tube atomic absorption spectrophotometry after oxine-MIBK and APDC-MIBK extraction].
Scalp hair samples were decomposed by the semimicro wet digestion method and transferred into test tubes (15 ml) equipped with face abraded exchangeable stoppers. To the test solution (5 ml), citric buffer was added and the pH was adjusted to 3.0, and water was added to make the solution 10 ml. Iron and other few ions are extracted with 1% oxine-MIBK solution (1 ml) and removed. Then, lead was extracted with APDC-MIBK solution (1 ml), and the upper layer (20 microliters) was injected immediately into graphite-tube. Atomic absorption was measured at 2,170 A corrected background by D2 lamp. Recovery tests of lead added to hair samples were satisfactory and interference by coexisting metals was not observed. With this method, the amount of lead in hair samples from healthy persons were determined, and the mean values of 4.90 ppm in male and 8.13 ppm in female were indicated.
D006108:Graphite; D006197:Hair / Q000032:analysis*; D006801:Humans; D007564:Japan; D007854:Lead / Q000032:analysis*; D008742:Methyl n-Butyl Ketone; D008832:Microchemistry; D015125:Oxyquinoline; D011759:Pyrrolidines; D013054:Spectrophotometry, Atomic / Q000295:instrumentation / Q000379:methods*; D013859:Thiocarbamates
D011759:Pyrrolidines; D013859:Thiocarbamates; C020972:pyrrolidine dithiocarbamic acid; D007854:Lead; D015125:Oxyquinoline; D008742:Methyl n-Butyl Ketone; D006108:Graphite; C005458:methyl isobutyl ketone