Ovomucoid and ovoinhibitor isolated from chicken egg white are immunologically cross-reactive.
Ovomucoid and ovoinhibitor were isolated from chicken egg white. Enzyme-linked immunosorbent assay (ELISA) and affinity chromatography of ovoinhibitor-coupled Sepharose 4B showed that about 25% of rabbit anti-ovomucoid antibody reacted with ovoinhibitor. Ovoinhibitor required about 1000 times the concentration of ovomucoid to give 50% inhibition of anti-ovomucoid antibody binding to ovoinhibitor in the plate-binding ELISA. These results suggested that ovoinhibitor possesses antigenic determinants which are conformationally homologous with those of ovomucoid and cross-react with anti-ovomucoid antibodies.
D000818:Animals; D002645:Chickens; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004527:Egg Proteins / Q000276:immunology / Q000302:isolation & purification*; D015387:Egg Proteins, Dietary; D004529:Egg White; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007700:Kinetics; D010059:Ovomucin / Q000276:immunology / Q000302:isolation & purification*; D011480:Protease Inhibitors / Q000276:immunology / Q000302:isolation & purification*
D004527:Egg Proteins; D015387:Egg Proteins, Dietary; D011480:Protease Inhibitors; D010059:Ovomucin; C004224:ovoinhibitor