In vivo distributions of some component fractions of Tc(NaBH4)-HEDP mixtures separated by anion exchange high performance liquid chromatography.
Anion exchange high performance liquid chromatography has been utilized to separate the component Tc-HEDP complexes of "carrier added" as well as "no carrier added" 99mTc(NaBH4)-HEDP reaction mixtures. The chromatographically separated "carrier added" Tc-HEDP components exhibit distinctly different biodistributions which are directly related to the chromatographic characteristics of the individual components. The Tc-HEDP complexes with shortest retention times, and therefore the lowest negative "charge density" have the maximum uptake on the bone, minimum uptake in the kidney, and fastest blood clearance.
D000818:Animals; D001842:Bone and Bones / Q000000981:diagnostic imaging; D001894:Borohydrides / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D012968:Etidronic Acid / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D007668:Kidney / Q000000981:diagnostic imaging; D007700:Kinetics; D008099:Liver / Q000000981:diagnostic imaging; D009132:Muscles / Q000000981:diagnostic imaging; D015609:Organotechnetium Compounds; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains; D013667:Technetium / Q000302:isolation & purification / Q000378:metabolism*; D014018:Tissue Distribution
D001894:Borohydrides; D015609:Organotechnetium Compounds; C019561:technetium Tc 99m etidronate; D013667:Technetium; C025364:sodium borohydride; D012968:Etidronic Acid