Organ culture of the crista ampullaris of the embryonic guinea pig.
The embryonic inner ear of the guinea pig was cultured one to ten days in vitro. The explantation occurred on the 30th and 40th gestational days, respectively. Hair cells were found in various stages of cytodifferentiation. Both hair cells and supporting cells were ultrastructurally preserved at the end of the period in vitro. The gross morphologic features of the crista ampullaris were still immature. The embryonic inner ear of the guinea pig can be maintained in organ culture until at least as late as the 46th gestational day.
D000375:Aging; D000818:Animals; D002454:Cell Differentiation; D007758:Ear, Inner / Q000196:embryology* / Q000648:ultrastructure; D006168:Guinea Pigs / Q000196:embryology*; D006198:Hair Cells, Auditory / Q000196:embryology / Q000648:ultrastructure; D008854:Microscopy, Electron; D009924:Organ Culture Techniques; D010865:Pilot Projects