The purification and function of acetyl coenzyme A:acyl carrier protein transacylase.
When individual enzyme activities of the fatty acid synthetase (FAS) system were assayed in extracts from five different plant tissues, acetyl-CoA:acyl carrier protein (ACP) transacylase and beta-ketoacyl-ACP synthetases I and II had consistently low specific activities in comparison with the other enzymes of the system. However, two of these extracts synthesized significant levels of medium chain fatty acids (rather than C16 and C18 acid) from [14C]malonyl-CoA; these extracts had elevated levels of acetyl-CoA:ACP transacylase. To explore the role of the acetyl transacylase more carefully, this enzyme was purified some 180-fold from spinach leaf extracts. Varying concentrations of the transacylase were then added either to spinach leaf extracts or to a completely reconstituted FAS system consisting of highly purified enzymes. The results suggested that: (a) acetyl-CoA:ACP transacylase was the enzyme catalyzing the rate-limiting step in the plant FAS system; (b) increasing concentration of this enzyme markedly increased the levels of the medium chain fatty acids, whereas increase of the other enzymes of the FAS system led to increased levels of stearic acid synthesis; and (c) beta-ketoacyl-ACP synthetase I was not involved in the rate-limiting step. It is suggested that modulation of the activity of acetyl-CoA:ACP transacylase may have important implications in the type of fatty acid synthesized, as well as the amount of fatty acids formed.
D000123:Acetyltransferases / Q000302:isolation & purification*; D051051:Acyl-Carrier Protein S-Acetyltransferase; D001151:Arsenic / Q000494:pharmacology; D018053:Arsenites; D064429:Fatty Acid Synthases / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D010944:Plants / Q000201:enzymology*; D013045:Species Specificity; D013379:Substrate Specificity
D018053:Arsenites; D000123:Acetyltransferases; D051051:Acyl-Carrier Protein S-Acetyltransferase; D064429:Fatty Acid Synthases; C015001:arsenite; D001151:Arsenic