Exogenous gangliosides enhance the interaction of fibronectin with ganglioside-deficient cells.
The major cell-surface glycoprotein fibronectin mediates a variety of cellular adhesive interactions that have been reported to be competitively inhibited by gangliosides. These effects suggest a possible function of gangliosides as receptors for fibronectin. To test this hypothesis more directly, we examined the interaction of endogenous fibronectin with a ganglioside-deficient cell line, NCTC 2071. These cells, which grow in serum-free medium, synthesized fibronectin. The fibronectin did not bind to these cells, but instead bound diffusely to the culture substratum. When the cells were cultured in medium containing ganglioside, the fibronectin became bound to the cell surface in fibrillar strands. The order of effectiveness of purified gangliosides was GT1b greater than GD1a greater than GM1 greater than GM2 greater than GM3. The effect with mixed gangliosides was accompanied by a restoration of cellular capacity to bind and to respond to cholera toxin. Treatment of the cells with several phospholipids did not alter fibronectin binding. Our results support the hypothesis that gangliosides can help mediate the binding of fibronectin to fibroblasts.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005353:Fibronectins / Q000378:metabolism*; D005677:G(M1) Ganglioside / Q000494:pharmacology; D005678:G(M2) Ganglioside / Q000494:pharmacology; D005679:G(M3) Ganglioside / Q000494:pharmacology; D005732:Gangliosides / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D013329:Structure-Activity Relationship
D005353:Fibronectins; D005679:G(M3) Ganglioside; D005732:Gangliosides; C021298:ganglioside, GD1a; D005678:G(M2) Ganglioside; D005677:G(M1) Ganglioside; C016481:trisialoganglioside GT1