title: UPD-galactose:ceramide galactosyltransferase activity in dissociated cell cultures from brain hemispheres of newborn rats.
abstract: Primary cultures enriched in oligodendroglial cells were prepared from dissociated newborn rat brain. Enzymatic study revealed the presence of high specific activities of UDP-galactose:ceramide galactosyltransferase (CGalT) and 2',3'-cyclic nucleotide-3'-phosphohydrolase (CNP). The specific activity of CGalT was twice as high as that of brain. The CGalT activity increased from 16 days in culture, reached a maximum at about 50 days and declined thereafter. The CNP activity reached a maximum after 60-70 days in culture and remained more or less stable in the following period. The results indicate that in oligodendroglial cells in vitro the regulation of CGalT activity proceeds in a manner similar to that of myelinating brain.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000201:enzymology*; D002478:Cells, Cultured; D005700:Galactosyltransferases / Q000378:metabolism*; D051147:Ganglioside Galactosyltransferase; D009457:Neuroglia / Q000201:enzymology*; D009836:Oligodendroglia / Q000201:enzymology*; D051381:Rats
chemical_list: D005700:Galactosyltransferases; D051147:Ganglioside Galactosyltransferase