The characterization of an adenosine deaminase from chicken serum.
1. Adenosine deaminase from chicken serum was purified and characterized. 2. The Km and Vmax values of the enzyme in respect of adenosine as substrate were 7.14 x 10(-3) M and 8.5 microM/min respectively. AMP, ADP & ATP are poorly metabolized respectively in decreasing order. 3. The enzyme had no NADase and NADPase activities but the presence of either NAD or NADP in normal incubation mixture activated the enzyme. 4. Metal ions Zn2+, Ca2+ and Mg2+ activated the enzyme to various levels. 5. Some compounds, mercaptoethanol, dithiothreitol (DTT), dithiobisnitrobenzoate (DTNB) inhibited the enzyme to various extents.
D000243:Adenosine Deaminase / Q000097:blood*; D000818:Animals; D002645:Chickens / Q000097:blood*; D005260:Female; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000494:pharmacology; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009700:Nucleoside Deaminases / Q000097:blood*; D013379:Substrate Specificity; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D013696:Temperature
D008670:Metals; D013439:Sulfhydryl Reagents; D009243:NAD; D009249:NADP; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase