Properties of the increased glutathione S-transferase A form in rat preneoplastic hepatic lesions induced by chemical carcinogens.
Glutathione S-transferase A form (GST-A) is increased markedly in rat preneoplastic hepatic lesions such as hyperplastic nodules induced by diethylnitrosamine followed by administration of N-2-fluorenylacetamide. GST-A was also significantly increased in livers of rats after short-term administration of some drugs. The increased activity and protein content of GST-A were demonstrated by CM-Sephadex C-50 column chromatography as well as by two-dimensional polyacrylamide gel electrophoresis following immuno-affinity column chromatography using antibody against GST-A. Immunologically, GST-A crossreacted strongly with GST-C, weakly with GST-C2, but not with ligandin, GST-B, or GST-AA. It was confirmed by subunit recombination that GST-C is a heterodimer composed of the subunits of homodimers, GST-A and GST-C2.
D015073:2-Acetylaminofluorene / Q000494:pharmacology; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D004052:Diethylnitrosamine / Q000494:pharmacology; D005982:Glutathione Transferase / Q000378:metabolism*; D007120:Immunochemistry; D008099:Liver / Q000201:enzymology*; D008113:Liver Neoplasms / Q000201:enzymology*; D008297:Male; D011230:Precancerous Conditions / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
D002273:Carcinogens; D004052:Diethylnitrosamine; D015073:2-Acetylaminofluorene; D005982:Glutathione Transferase