In vitro formation of different tubulin polymers from purified tubulin of Ehrlich ascites tumor cells.
Preparations of cycled tubulin from Ehrlich ascites tumor cells contain several accessory proteins; once or twice cycled microtubule preparations are usually composed of fibers 10 nm in diameter, but lack vimentin. Highly purified tubulin consists of alpha- and beta-tubulin and a minor component which was identified by peptide mapping as a second beta-chain. This pure tubulin is able to form in vitro at low concentrations (1 mg protein/ml) fibers of about 10 nm width, and at higher concentrations (3.5 mg protein/ml) normal microtubules.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000648:ultrastructure*; D046911:Macromolecular Substances; D051379:Mice; D008854:Microscopy, Electron; D008870:Microtubules / Q000648:ultrastructure*; D008970:Molecular Weight; D014404:Tubulin / Q000302:isolation & purification / Q000378:metabolism*
D046911:Macromolecular Substances; D014404:Tubulin