Complexes between mitochondrial enzymes and either citrate synthase or glutamate dehydrogenase.
Experiments performed in polyethylene glycol and with a divalent crosslinker indicate that both mitochondrial malate dehydrogenase and aspartate aminotransferase can form hetero enzyme--enzyme complexes with either glutamate dehydrogenase or citrate synthase. In general, these as previous results indicate that complexes with the aminotransferase are favored over those with malate dehydrogenase and complexes with glutamate dehydrogenase are favored over those with citrate synthase. When the levels of enzymes are low, the only detectable complex is between the aminotransferase and glutamate dehydrogenase. Under these conditions, palmitoyl-CoA is required for complexes between the other three enzyme pairs, however, palmitoyl-CoA also enhances interactions between glutamate dehydrogenase and the aminotransferase. DPNH disrupts complexes with malate dehydrogenase and has little effect on those with the aminotransferase, while oxalacetate disrupts complexes with citrate synthase but has little effect on those with glutamate dehydrogenase. The citrate synthase-aminotransferase complex was favored in the presence of DPNH plus malate, which disrupt the other three enzyme-enzyme complexes. Glutamate dehydrogenase has a higher affinity and capacity than citrate synthase for palmitoyl-CoA. Consequently, lower levels of palmitoyl-CoA are required to enhance interactions with glutamate dehydrogenase. Furthermore, glutamate dehydrogenase can compete with citrate synthase for palmitoyl-CoA and thus can prevent palmitoyl-CoA from enhancing interactions between citrate synthase and either malate dehydrogenase or the aminotransferase.
D000214:Acyl Coenzyme A / Q000494:pharmacology*; D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D001667:Binding, Competitive; D002417:Cattle; D002950:Citrate (si)-Synthase / Q000378:metabolism*; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology*; D007652:Oxo-Acid-Lyases / Q000378:metabolism*; D010171:Palmitoyl Coenzyme A / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D013552:Swine
D000214:Acyl Coenzyme A; D010171:Palmitoyl Coenzyme A; D005969:Glutamate Dehydrogenase; D002950:Citrate (si)-Synthase; D007652:Oxo-Acid-Lyases