Extraction of phospholipids from aldehyde-fixed membranes.
The effect of aldehyde fixation on membrane lipids and proteins has been studied. Purified fractions of toad retinal receptor outer segments were treated with a nonionic detergent after aldehyde fixation. Glutaraldehyde-fixed material retained proteins and phosphatidyl serine while other phospholipids were solubilized and extracted by detergent. Since phosphatidyl ethanolamine is extracted it is proposed tha the mechanism of phosphatidyl serine retention does not depend on its amine groups. Formaldehyde fixed material was not able to retain its proteins when treated with detergent. The difference in protein retention between formaldehyde and glutaraldehyde may be due to the formation of a supramolecular network in the presence of the two reactive groups in glutaraldehyde.
D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D002025:Bufonidae; D002623:Chemistry Techniques, Analytical / Q000379:methods; D005557:Formaldehyde / Q000378:metabolism / Q000494:pharmacology; D005976:Glutaral / Q000378:metabolism / Q000494:pharmacology; D008563:Membrane Lipids / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D010743:Phospholipids / Q000302:isolation & purification*; D012160:Retina / Q000378:metabolism*; D012243:Rhodopsin / Q000378:metabolism
D000447:Aldehydes; D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids; D005557:Formaldehyde; D012243:Rhodopsin; D005976:Glutaral