Eukaryotic RNA polymerase II binds to nucleosome cores from transcribed genes.
Purified RNA polymerase II from calf thymus can bind to about 15% of the nucleosome core particles prepared from mouse myeloma cells, forming a discrete complex having a sedimentation coefficient of 18S. These bound nucleosome cores are heavily enriched in transcribed DNA sequences, are deficient in histones H2A and H2B, and undergo a reversible change in structure on RNA polymerase II binding.
D000818:Animals; D002417:Cattle; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D005796:Genes; D006657:Histones / Q000235:genetics; D051379:Mice; D009707:Nucleosomes / Q000502:physiology* / Q000648:ultrastructure; D011485:Protein Binding; D012319:RNA Polymerase II / Q000235:genetics*; D014158:Transcription, Genetic
D006657:Histones; D009707:Nucleosomes; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases