Decreased viscosity of rat-liver DNA treated by 3'-methyl-4-dimethylaminoazobenzene, detected with a new viscometric approach.
DNA damage induced in vivo by 3'-methyl-4-dimethylaminoazobenzene (3'CH3DAB) was investigated with 2 differently sensitive techniques: the alkaline elution assay and the viscometric measurement of DNA damage. 3'CH3DAB appeared to be falsely negative with the alkaline elution assay, whereas with the viscometric approach, which is about 30-50 times more sensitive, it appeared positive, and the DNA damage was dose-dependent.
D000818:Animals; D004247:DNA; D008099:Liver / Q000032:analysis; D008297:Male; D008722:Methods; D008749:Methyldimethylaminoazobenzene / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D014783:Viscosity; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives*
D008749:Methyldimethylaminoazobenzene; D004247:DNA; D004124:p-Dimethylaminoazobenzene