[Effect of slow rates of freezing on the functional state of mitochondria from various types of tissue preparations of kidney cortex].
Changes in functional activity of mitochondria, in concentration of respiration enzymes and the state of internal membrane after slow freezing down to -5 degrees, -12 degrees, -21 degrees and -30 degrees of various tissue preparations of renal cortex (homogenates, slices, etc) were studied. The following phenomena were found: an increase in the rate of mitochondrial respiration at the second metabolic state, inhibition of electron transport most distinct during mitochondrial oxidation of alpha-ketoglutarate, a decrease in concentration of cytochromes, an increase in the internal membrane permeability. Considerable damage of mitochondria was observed at temperatures between -5 degrees and -12 degrees but the most pronounced impairment of the organelles occurred at -10 degrees -20 degrees, i. e. at the freezing point of free water in the suspension and at eutectic temperatures.
D000818:Animals; D003580:Cytochromes / Q000378:metabolism; D004579:Electron Transport; D005260:Female; D005615:Freezing; D007672:Kidney Cortex / Q000378:metabolism* / Q000648:ultrastructure; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D051381:Rats
D003580:Cytochromes
Vliianie medlennykh skorosteĭ zamorazhivaniia na funktsional'noe sostoianie mitokhondriĭ raznykh tipov tkanevykh preparatov kory pochki.