The use of CIE for the detection of Clostridium difficile toxin in stool filtrates: laboratory and clinical correlation.
Two CIE procedures (CIE-1, CIE-2) for the detection of Clostridium difficile in diarrheal stools were evaluated by comparison to cytotoxin assay and culture results and by comparison to a clinical likelihood of C. difficile-induced diarrhea. Using a combination of toxin assay and culture results for reference, the CIE-1 and CIE-2 procedures had sensitivities of 33% and 47%, specificities of 89% and 91%, and positive predictive values of 42% and 54%, respectively. Using clinical likelihood for reference, the best results were obtained by the CIE-2 method, which yielded a positive predictive value of 77%. Neither CIE procedure provided an acceptable sensitivity for the detection of C. difficile in stools.
D000328:Adult; D000942:Antigens, Bacterial / Q000032:analysis*; D001427:Bacterial Toxins / Q000032:analysis*; D003013:Clostridium / Q000276:immunology*; D003015:Clostridium Infections / Q000175:diagnosis; D003378:Counterimmunoelectrophoresis / Q000379:methods; D046508:Culture Techniques; D003603:Cytotoxins / Q000032:analysis; D003967:Diarrhea / Q000276:immunology; D005069:Evaluation Studies as Topic; D005243:Feces / Q000032:analysis / Q000382:microbiology*; D006801:Humans
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D003603:Cytotoxins