Radioimmunologic identification of prostaglandins produced by serum-stimulated mouse embryo palate mesenchyme cells.
High-performance liquid chromatography and radioimmunoassay were used to identify the prostaglandins synthesized by mouse embryo palate mesenchyme cells. Serum stimulated the release of several different metabolites of arachidonic acid including 6-ketoprostaglandin F1 alpha (the stable product of prostacyclin, prostaglandin I2), prostaglandin E2 and prostaglandin F2 alpha. Compared to control cells, the serum-stimulated cells produce elevated levels of prostaglandin E2 (36-fold), 6-ketoprostaglandin F1 alpha (15-fold) and prostaglandin F2 alpha (7-fold). The acetylenic analogue of arachidonic acid, 5,8,11,14-eicosatetraynoic acid prevented this accelerated synthesis.
D015117:5,8,11,14-Eicosatetraynoic Acid / Q000494:pharmacology; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001769:Blood; D002851:Chromatography, High Pressure Liquid; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D010159:Palate / Q000166:cytology* / Q000196:embryology; D011247:Pregnancy; D011453:Prostaglandins / Q000096:biosynthesis*; D011863:Radioimmunoassay
D001095:Arachidonic Acids; D011453:Prostaglandins; D015117:5,8,11,14-Eicosatetraynoic Acid; D016718:Arachidonic Acid