title: A genetic map of reovirus: assignment of the newly defined mutant groups H, I, and J to genome segments.
abstract: Mutants representing three previously undefined reovirus type 3 mutant groups have been isolated following backcross of suppressed pseudorevertants to wild type (R.F. Ramig and B.N. Fields, 1979, Virology 92, 155-167; R. Ahmed, P.R. Chakraborty, A.F. Graham, R.F. Ramig, and B.N. Fields, 1980, J. Virol. 34, 383-389). The prototype mutant of each of the three new mutant groups was mapped by analysis of genome segment segregation in intertypic recombinants derived from crosses between the type 3 ts mutants and ts mutants of type 1 or type 2. Segregation analysis revealed the location of the group H prototype mutant tsH(26/8) to be genome segment M1, that of the group I prototype mutant tsI(138) to be segment L3, and that of the group J prototype mutant tsJ(128) to be segment S1. Mapping of the group I and J lesions required the identification of suppressed ts lesions in some of the intertypic rcombinant clones.
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D051379:Mice; D009154:Mutation; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D012087:Reoviridae / Q000235:genetics*
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral