title: CA2+ uptake in an asporogenous mutant strain of Bacillus megaterium.
abstract: Some properties of Ca2+ accumulation were examined in Bacillus megaterium mutant 3-12 which cannot sporulate owing to a lack of dipicolinic acid. The steady-state intracellular content of Ca2+ was half-saturated at 0.06 mM. Ca2+ was accumulated up to its plateau in 30 min. There were two classes of Ca2+ accumulation, high-affinity and low-affinity, both of which were mostly energy-dependent, and there was a small amount of energy independent Ca2+ binding. Exchange of Ca2+ accumulated in the cells for the external Ca2+ in the medium was observed. The highest rates of exchange were evident in the cells harvested when Ca2+ accumulation reached its peak.
mesh_terms: D001410:Bacillus megaterium / Q000235:genetics / Q000378:metabolism*; D001412:Bacillus subtilis / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D007811:Lanthanum / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism; D009154:Mutation; D010085:Oxidative Phosphorylation; D013171:Spores, Bacterial
chemical_list: D007811:Lanthanum; D002118:Calcium