Liposomal blockade of the reticuloendothelial system: improved tumor imaging with small unilamellar vesicles.
The reticuloendothelial system of mice bearing EMT6 tumors was effectively blocked by intravenous injections of small unilamellar vesicles that incorporated a 6-aminomannose derivative of cholesterol in the lipid bilayer. Neutral liposomes loaded with indium-111-nitrilotriacetic acid were then injected. Fifty percent more radioactivity was deposited in tumors of the animals with blocked reticuloendothelial systems than in controls. Twenty-four hours after the injection of radioactive vesicles, well-defined tumor images were observed in whole-body gamma camera scintigraphs. Biodistribution studies showed that tumors from animals with blocked reticuloendothelial systems had more than twice the radioactivity per gram than any other tissue analyzed.
D000818:Animals; D007204:Indium; D008081:Liposomes / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012157:Mononuclear Phagocyte System / Q000503:physiopathology*; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000000981:diagnostic imaging*; D009374:Neoplasms, Experimental / Q000000981:diagnostic imaging; D009571:Nitrilotriacetic Acid; D011868:Radioisotopes; D011877:Radionuclide Imaging / Q000379:methods
D008081:Liposomes; D011868:Radioisotopes; D007204:Indium; D009571:Nitrilotriacetic Acid