Changes in glycosidase activities in lectin resistant myogenesis-defective myoblast cell lines.
1. Mannosidase, hexosaminidase, galactosidase and fucosidase activities were determined in cell free extracts of wild type and con A-resistant L6 myoblast cells. 2. Two mannosidase activities were detected, with pH optima of 4.6 and 6.0. The con A-resistant cells were almost devoid of intracellular mannosidase activity with optimum pH 4.6. Differences in Km and Vmax values for mannosidase activities were found when con A-resistant and wild type cells were compared. 3. A decrease in the intracellular hexosaminidase activity in the con A-resistant myoblasts was accompanied by an increase in extracellular activity. No differences were observed in the extracellular mannosidase activities of wild type and con A-resistant cells.
D000818:Animals; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D004351:Drug Resistance; D006026:Glycoside Hydrolases / Q000378:metabolism*; D037102:Lectins / Q000494:pharmacology*; D024510:Muscle Development; D009132:Muscles / Q000201:enzymology*; D010641:Phenotype; D051381:Rats
D037102:Lectins; D003208:Concanavalin A; D006026:Glycoside Hydrolases