Presence of prostaglandins (PGs) in Tetrahymena pyriformis, GL and the effect of aspirin.
Tetrahymena pyriformis GL appears to require prostaglandins, either B, E or F series for growth, as demonstrated by the deleterious effect of aspirin. The latter inhibits prostaglandin synthetase (cyclooxygenase). Aspirin inhibited 50% growth of a 24 hr culture of T. pyriformis at a dose of approximately 200 micrograms/ml and completely inhibited at 600 micrograms/ml. Extraction with acid ethyl acetate: isopropanol solvent of 2.8 x 10(8) cells yielded 41.3 mg of lipid of which 62.8% and 34.5% were PGE2 and PGB, respectively. It is suggested that PGs are important for the growth of T. pyriformis and that the organism may be a useful source of natural PGs. Additionally, T. pyriformis may be useful in studies of the PGs pathway.
D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D015232:Dinoprostone; D011453:Prostaglandins / Q000378:metabolism*; D011456:Prostaglandins B / Q000378:metabolism; D011458:Prostaglandins E / Q000378:metabolism; D013769:Tetrahymena pyriformis / Q000187:drug effects / Q000378:metabolism*
D011453:Prostaglandins; D011456:Prostaglandins B; D011458:Prostaglandins E; D015232:Dinoprostone; D001241:Aspirin