A gene linked to the heavy-chain allotype I alpha (1a) controls for the expression of idiotype Ox-r1 in the rat.
A rabbit antiserum prepared against purified anti-2-phenyl-oxazolone (phOx) antibodies of AVN rats detects a common idiotype called Ox-r1. This idiotype was also present in anti-phOx antibodies of AS and (AS x LEP)F1 hybrid rats but not in the antibodies of LEP rats. Antisera of (AS x LEP) x LEP backcross rats either resembled F1 hybrid sera (7 rats) or LEP sera (8 rats) in the idiotype assay. All idiotype-positive backcross rats but none of the idiotype negative backcross rats had the I alpha (1) allotype a. The likelihood that such a distribution was due to a chance is approximately 0.0002. The data suggest that the presence of idiotype Ox-r1 in the antisera of AS rats and their progeny is controlled by a gene (or genes) linked to the 1g heavy-chain allotype locus I alpha (1).
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D005260:Female; D006824:Hybridization, Genetic; D007126:Immunoglobulin Allotypes / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007130:Immunoglobulin Idiotypes / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007525:Isoelectric Focusing; D008297:Male; D011863:Radioimmunoassay; D051381:Rats / Q000235:genetics*
D000888:Antibodies, Anti-Idiotypic; D007126:Immunoglobulin Allotypes; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007147:Immunoglobulin Light Chains