title: Simple and rapid chromatographic separation of IgM using microcolumns and stained sera.
abstract: By adding two different stains to samples of serum it was possible to monitor visually the chromatographic separation of the IgM antibody-containing fraction from other serum components and to collect this fraction manually. The use of microcolumns filled with a medium of very low resistance of flow reduced drastically the time for the separation. A simple apparatus is described which allows to run up to 10 columns in periods of 3 min. Using this technique, IgM antibody-containing fractions from 20 sera can be obtained in 2 h. The efficiency of the method was demonstrated using sera from 55 patients with hepatitis A infection.
mesh_terms: D000914:Antibodies, Viral / Q000302:isolation & purification; D002850:Chromatography, Gel / Q000379:methods; D006506:Hepatitis A / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification*; D011863:Radioimmunoassay
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M