Transformation of 12-deoxycardenolids with Streptomyces purpurascens.
Streptomyces purpurascens transforms primary and secondary 12-deoxycardenolids into digoxin, 7 beta-hydroxy-digitoxin and 7 beta-hydroxy-digoxin, respectively. A metabolic pathway is proposed for cardenolid transformations and the enzymes participating in it are investigated. The substrate concentration in the medium can be increased by using a water-miscible solvent of electron donor character. The newly developed procedure yields 0.5 g/1(-1) of digoxin in a five-day fermentation.
D000581:Amidohydrolases / Q000378:metabolism; D001711:Biotransformation; D002301:Cardiac Glycosides / Q000378:metabolism*; D004074:Digitoxin / Q000378:metabolism*; D004077:Digoxin / Q000378:metabolism; D007799:Lanatosides / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism; D013302:Streptomyces / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism
D002301:Cardiac Glycosides; D007799:Lanatosides; C018548:lanatoside C; D004077:Digoxin; D004074:Digitoxin; D006899:Mixed Function Oxygenases; D001617:beta-Glucosidase; D000581:Amidohydrolases; C036572:lanatoside A