Immunoadsorption to improve gas chromatography/high-resolution mass spectrometry of estradiol-17 beta in plasma.
We describe a new, highly selective procedure for the determination of estradiol-17 beta in plasma. Samples are extracted with a micro-cellulose-coupled antiserum to estradiol-17 beta. Conversion of the extracted steroid to the bis(trimethylsilyl) ether is followed by gas chromatography/high-resolution mass spectrometry with selected ion monitoring. Precise quantification is achieved through the use of [2H3]estradiol-17 beta as internal standard.
D003429:Cross Reactions; D004958:Estradiol / Q000097:blood* / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D007164:Immunosorbents
D007164:Immunosorbents; D004958:Estradiol