The relationship between NADPH-dependent lipid peroxidation and degradation of cytochrome P-450 in adrenal cortex mitochondria.
The relationship between NADPH-dependent lipid peroxidation and the degradation of cytochrome P-450 has been studied in bovine adrenal cortex mitochondria. Malondialdehyde formation is accompanied by a corresponding decrease in total cytochrome P-450 content. Inhibitors of lipid peroxidation also prevent the loss of cytochrome P-450, further demonstrating a direct relationship between NADPH-dependent lipid peroxidation and degradation of P-450. To differentiate between cytochrome P-450(11)beta and P-450scc, steroid-induced difference spectra were used to evaluate P-450 degradation. These measurements provide the first evidence that both P-450's are degraded during NADPH-dependent lipid peroxidation with P-450(11)beta being much more susceptible to this process.
D000302:Adrenal Cortex / Q000201:enzymology*; D000818:Animals; D002417:Cattle; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003900:Desoxycorticosterone / Q000494:pharmacology; D006888:Hydroxycholesterols / Q000494:pharmacology; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology; D009249:NADP / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D013053:Spectrophotometry
D006888:Hydroxycholesterols; D008054:Lipid Peroxides; D008563:Membrane Lipids; D003900:Desoxycorticosterone; C025813:20-hydroxycholesterol; D009249:NADP; D003577:Cytochrome P-450 Enzyme System