title: Parathyroid hormone stimulates adenylate cyclase in rat cerebral microvessels.
abstract: We have studied the effect of parathyroid hormone (PTH) on adenylate cyclase of microvessels isolated from rat cerebral cortex. Native bovine (b) PTH-(1-84), the synthetic amino-terminal fragment bPTH-(1-34) and the synthetic analog [Nle8,Nle18,Tyr34]-bPTH- (1-34) amide stimulated adenylate cyclase in a dose-dependent manner with apparent ED50 values of 16 nM, 6.3 nM and 15 nM respectively. The stimulation by bPTH was greatly enhanced by guanosine triphosphate. The PTH antagonist, [Nle8,Nle18,Tyr34]-bPTH-(3-34) amide inhibited the action of bPTH-(1-84) and bPTH-(1-34). In summary, PTH stimulated adenylate cyclase in rat cerebral microvessels in a very similar manner to its stimulation in the renal cortex.
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008833:Microcirculation; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D010281:Parathyroid Hormone; D010446:Peptide Fragments; C025076:parathyroid hormone (1-34), bovine; C025497:parathyroid hormone (3-34) amide, Nle(8,18)-Nle(34)-; D011433:Propranolol; D000262:Adenylyl Cyclases; D007545:Isoproterenol; D010646:Phentolamine