Cyclobenzaprine effects on locus coeruleus cells in tissue slice.
Tissue slices 400 mu thick were taken from the brain stem, at the level of the locus coeruleus, of 150-250 g Sprague-Dawley rats. Microelectrodes were placed in the locus coeruleus under visual control, and a cell whose discharge rate would decrease with microiontophoretic application of norepinephrine or clonidine was sought. Cyclobenzaprine (CBZ) was then introduced into the perfusion medium at a concentration equivalent to 1 mg/kg body weight of the whole animal. Discharge rates before and during CBZ administration were compared. The six cells with initial discharge rates between 2 and 10 Hz decreased firing with CBZ, whereas the four cells with initial rates between 0.5 and 1.5 Hz increased their rates with CBZ.
D000200:Action Potentials / Q000187:drug effects; D000639:Amitriptyline / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D003000:Clonidine / Q000494:pharmacology; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008125:Locus Coeruleus / Q000187:drug effects* / Q000502:physiology; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D000639:Amitriptyline; C004704:cyclobenzaprine; D003000:Clonidine; D009638:Norepinephrine