Hypoxic cell radiosensitization by moderate hyperthermia and glucose deprivation.
Cell culture studies were carried out to determine whether moderate hyperthermia reduces the oxygen enhancement ratio of cells under well-defined cultural conditions. Using asynchronously growing HeLa cells, the OER of cells with and without glucose was determined following exposure of cells to moderate hyperthermia, 40.5 degrees C for 1 hr, immediately after X irradiation. The OER of cells with 5 mM glucose was 3.2, whereas the OER of glucose-deprived cells was reduced to 2.0. The pH of the cell culture medium was kept at 7.4 throughout the experiments. The present finding may provide a clue toward further enhancing the radiosensitization of hypoxic cells by heat.
D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005947:Glucose / Q000494:pharmacology*; D006367:HeLa Cells; D006358:Hot Temperature / Q000627:therapeutic use; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010100:Oxygen; D011836:Radiation Tolerance; D013997:Time Factors; D014965:X-Rays
D005947:Glucose; D010100:Oxygen