Protease inhibitors suppress the survival increase mediated by uncouplers in X-irradiated mammalian cells.
When mammalian cells are incubated with an uncoupler of oxidative phosphorylation prior to and during X-irradiation, the survival and the mutation frequency are markedly increased. This process requires protein synthesis and is inhibited when the cells are plated in the presence of a protease inhibitor (antipain or leupeptin). These results suggest the existence of an error-prone DNA repair process in X-irradiated mammalian cells.
D000818:Animals; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects*; D006224:Cricetinae; D003412:Cricetulus; D003513:Cycloheximide / Q000494:pharmacology; D004260:DNA Repair; D005260:Female; D009154:Mutation; D010053:Ovary; D011480:Protease Inhibitors / Q000494:pharmacology*
D011480:Protease Inhibitors; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D003513:Cycloheximide