Improved method for purification of membranes from spores of Bacillus Megaterium.
An improved method for spore membranes isolation was developed based on sucrose density gradient centrifugation in a vertical rotor. The advantage of this over previous methods was the complete removal of RNA and a 40% reduction in protein content, while retaining the high specific activities for membrane bound dehydrogenases and for amino acid uptake.
D000596:Amino Acids / Q000378:metabolism; D001410:Bacillus megaterium / Q000378:metabolism / Q000648:ultrastructure*; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure*; D002499:Centrifugation, Density Gradient / Q000379:methods; D008563:Membrane Lipids / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D013171:Spores, Bacterial / Q000648:ultrastructure
D000596:Amino Acids; D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids