A method for the hyaluronic acid synthetase assay in human skin.
A method for the determination of hyaluronic acid synthetase activity of human skin is described. Skin samples were crush-homogenized, and incubated with UDP-14C-glucuronic acid and UDP-N-acetylglucosamine in Tris-HCl-buffer containing MgCl2. After papain digestion of the samples, the 14C-labeled hyaluronic acid was separated in Sephadex G 50 chromatography. The radioactivity incorporated into hyaluronic acid was an expression of the activity of hyaluronic acid synthetase. This activity was found to be increased in skin biopsies obtained from psoriatic lesions and decreased after local treatment with potent corticoids.
D000305:Adrenal Cortex Hormones / Q000627:therapeutic use; D000328:Adult; D002250:Carbon Radioisotopes; D002850:Chromatography, Gel; D005260:Female; D014453:Glucuronosyltransferase / Q000032:analysis*; D016695:Glycosyltransferases; D006801:Humans; D000076002:Hyaluronan Synthases; D008565:Membrane Proteins; D011565:Psoriasis / Q000188:drug therapy / Q000201:enzymology; D012867:Skin / Q000201:enzymology*; D014166:Transferases; D014535:Uridine Diphosphate Glucuronic Acid; D029867:Xenopus Proteins
D000305:Adrenal Cortex Hormones; D002250:Carbon Radioisotopes; D008565:Membrane Proteins; D029867:Xenopus Proteins; D014535:Uridine Diphosphate Glucuronic Acid; D014166:Transferases; D016695:Glycosyltransferases; D014453:Glucuronosyltransferase; C056944:HAS1 protein, Xenopus; D000076002:Hyaluronan Synthases