Structure and metabolism of mammalian liver glycogen monitored by carbon-13 nuclear magnetic resonance.
Natural-abundance 13C NMR signals from glycogen are observable in situ within the perfused livers of rats. The nuclear magnetic relaxation properties (T1, T2, eta + 1) of glycogen were measured for glycogen in situ and in vitro and were found to be identical. All of the carbon nuclei in glycogen contribute to the high-resolution NMR spectrum, in spite of glycogen's very large molecular weight. The metabolism of glycogen in situ in the perfused rat liver was followed by 13C NMR. Stimulation of the fed rat liver by physiological glucagon levels led to rapid glycogenolysis. Perfusion of the liver with [1-13C]glucose led to net glycolysis, with concomitant scrambling of the label from C1 to C6 due to triosephosphate isomerase activity.
D000818:Animals; D002247:Carbon Isotopes; D005934:Glucagon / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D008112:Liver Glycogen / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D011817:Rabbits
D002247:Carbon Isotopes; D008112:Liver Glycogen; D005934:Glucagon; D005947:Glucose