Immunohistochemical demonstration of extracellular carbonic anhydrase in epiphyseal growth cartilage.
The distribution of carbonic anhydrase isoenzymes C and B in the rate epiphyseal growth cartilage was demonstrated by an immunohistochemical method. The isoenzymes were found in different locations. Isoenzyme C was in the extracellular matrix of the hypertrophic and calcifying cartilage, and no reaction was observed in the chondrocytes. In contrast, the antiserum against isoenzyme B revealed only a weak cellular staining. This supports the hypothesis that carbonic anhydrase isoenzyme C, which is the high-activity form, changes the pH in the extracellular fluid of calcifying cartilage, favoring the deposition of calcium phosphate.
D000818:Animals; D002256:Carbonic Anhydrases / Q000032:analysis* / Q000378:metabolism; D002356:Cartilage / Q000166:cytology / Q000201:enzymology*; D004838:Epiphyses / Q000201:enzymology*; D005260:Female; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D051381:Rats
D007527:Isoenzymes; D002256:Carbonic Anhydrases