Tay-Sachs disease and normal cerebellar cells in culture: elevated levels of lysosomal enzymes in Tay-Sachs disease cells.
Tay-Sachs disease and normal fetal cerebellar cells were maintained in culture for up to four weeks. Elevated levels of the lysosomal enzymes hexosaminidase B, beta-galactosidase, and acid phosphatase were observed in the Tay-Sachs cells in long term cultures although elevated lysosomal enzyme levels were not observed in the normal cultures. Similarly, elevated lysosomal enzyme activities were observed in Tay-Sachs cultures which had been treated with Concanavalin A.
D000135:Acid Phosphatase / Q000378:metabolism; D002478:Cells, Cultured; D002531:Cerebellum / Q000201:enzymology*; D005260:Female; D005333:Fetus; D006596:Hexosaminidases / Q000378:metabolism; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D007700:Kinetics; D008247:Lysosomes / Q000201:enzymology*; D011247:Pregnancy; D012016:Reference Values; D013661:Tay-Sachs Disease / Q000201:enzymology*; D001616:beta-Galactosidase / Q000378:metabolism
D006867:Hydrolases; D000135:Acid Phosphatase; D006596:Hexosaminidases; D001616:beta-Galactosidase