Isolation of the ends of La Crosse virus small RNA as a double-stranded structure.
The genome of La Crosse virus, a member of the Bunyaviridae, is made up of three molecules. Circular nucleocapsid structures, in three size classes, have been isolated from La Crosse virus (Obijeski et al. J. Virol. 20:664-675, 1976). Recently, Obijeski et al. (Nucleic Acids Res. 8:2431-2438) have found that the 5' and 3' ends of each segment are complementary in sequence. We determined that a 5' and 3' end complementary structure, predicted by the rules of Tinoco et al. (Nature [London] 230:362-367), can and will anneal under certain conditions. This structure is resistant to RNase in high-salt medium and can be isolated in a reasonably high yield.
D001483:Base Sequence; D002043:Bunyaviridae / Q000235:genetics*; D002141:Encephalitis Virus, California / Q000032:analysis / Q000235:genetics*; D005814:Genes, Viral; D012330:RNA, Double-Stranded / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification*
D012330:RNA, Double-Stranded; D012367:RNA, Viral