Time course of inhibition of synthesis and basal release of FSH from pituitary glands of female rats by bovine follicular fluid in vitro.
Inhibin-like activity in steroid-free bovine follicular fluid (bFF) is demonstrated using an in vitro technique with hemi-pituitary glands from intact female (second day of dioestrus) and ovariectomized rats: synthesis as well as basal release of FSH, but not of LH, are inhibited profoundly. The results confirm and extend data from other investigators on the action of inhibin-like material. The effect of the inhibin-like activity is shown to be reversible, as synthesis and the rise of basal release are restored when bFF is withdrawn from the incubation medium. Synthesis of FSH seems to be inhibited earlier than basal release, and it is suggested that the inhibin-like material acts only directly on FSH synthesis. Some possibilities of the mechanisms of action of inhibin-like activity are discussed.
D000818:Animals; D001826:Body Fluids / Q000032:analysis / Q000502:physiology*; D002369:Castration; D005260:Female; D005640:Follicle Stimulating Hormone / Q000096:biosynthesis*; D007265:Inhibins / Q000502:physiology; D007986:Luteinizing Hormone / Q000378:metabolism; D006080:Ovarian Follicle / Q000032:analysis / Q000502:physiology*; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007265:Inhibins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone