Purification and characterization of phosphatidylinositol-specific phospholipase C from bovine platelets.
Phosphatidylinositol-specific phospholipase C was purified to homogeneity from soluble fraction of bovine platelets by ammonium sulfate fractionation, hydrophobic chromatography, DEAE ion exchange chromatography and gel filtration. The purified enzyme has a narrow pH optimum ranging from 6.5 to 7.5 and the molecular weight of the enzyme was estimated to be 143,000 by sodium dodecyl sulfate slab gel electrophoresis. The purified enzyme requires Ca2+ strictly for activity, which was markedly enhanced in the presence of arachidonate. No enhancement of the activity was observed in the presence of purified calmodulin. The activity was markedly inhibited in the presence of quinacrine but no inhibition by indomethacin was observed.
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001792:Blood Platelets / Q000201:enzymology*; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008970:Molecular Weight; D010716:Phosphatidylinositols / Q000378:metabolism*; D010740:Phospholipases / Q000097:blood*; D013379:Substrate Specificity; D010738:Type C Phospholipases / Q000097:blood* / Q000302:isolation & purification
D001095:Arachidonic Acids; D010716:Phosphatidylinositols; D016718:Arachidonic Acid; D010740:Phospholipases; D010738:Type C Phospholipases