Selective crystallization of horse isoferritins.
Various precipitating agents were examined in order to crystallize horse heart and spleen ferritins. Cadmium sulfate induced the crystallization of the spleen ferritin, while 2-methyl-2,4-pentanediol and poly(ethylene glycol) only induced that of the heart ferritin. Isoelectric focusing analysis showed that the crystals grown from cadmium sulfate contained only the more acidic isoferritins, and those grown from methyl pentanediol only the less acidic isoferritins. Heart ferritin crystallizes in a cubic space group, as previously reported for spleen ferritin crystals grown from cadmium sulfate.
D000818:Animals; D002104:Cadmium; D019187:Cadmium Compounds; D003460:Crystallization; D005293:Ferritins / Q000302:isolation & purification*; D006018:Glycols; D006736:Horses; D009206:Myocardium / Q000032:analysis; D011092:Polyethylene Glycols; D013154:Spleen / Q000032:analysis; D013431:Sulfates
D019187:Cadmium Compounds; D006018:Glycols; D013431:Sulfates; D002104:Cadmium; D011092:Polyethylene Glycols; D005293:Ferritins; C037123:cadmium sulfate; C011480:hexylene glycol