Regulation of heat-shock genes: a DNA sequence upstream of Drosophila hsp70 genes is essential for their induction in monkey cells.
Heat-shock genes coding for heat-shock protein 70 (HSP70) in Drosophila melanogaster were subcloned into an SV40/plasmid recombinant capable of replication in permissive monkey COS cells. Following transfection of COS cells, no significant amount of Drosophila hsp70 RNA was detected at 37 degrees C. In contrast, a heat-shock at 43 degrees C or arsenite poisoning at 37 degrees C induced the massive production of Drosophila hsp70 RNA of correct size and faithful 5' ends. After heat-shock, the efficiency of hsp70 transcription in COS cells containing 2-4 X 10(4) gene copies was found to be 15-30% of that measured in Drosophila, on a per gene basis. By testing a series of 5' deletion mutants in this inducible transcription assay it was found that a sequence less than 70 bp long, directly upstream of the hsp70 gene, was essential for the heat or arsenite induction of transcription.
D000818:Animals; D001151:Arsenic / Q000633:toxicity; D018053:Arsenites; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004261:DNA Replication; D004331:Drosophila melanogaster / Q000235:genetics*; D005796:Genes / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D051381:Rats; D014158:Transcription, Genetic; D014162:Transfection
D018053:Arsenites; D006360:Heat-Shock Proteins; C015001:arsenite; D001151:Arsenic