A study on the dissolution and disintegration of calcium bilirubinate stones, with special reference to effects of litholytic agents in human bile and to irrigation of bile duct in dogs.
1) Slices of calcium bilirubinate stones were readily soluble in bile added with tetrasodium salt of ethylene-diaminetetraacetic acid (EDTA.4Na). 2) Bile added with 3 agents, EDTA.4Na, ursodeoxycholate (Urso), and heparin could dissolve calcium bilirubinate stones more easily than, or comparably to, the bile added only with EDTA.4Na. 3) The pH of the bile added with the combination of EDTA.4Na-Urso-heparin was 8.72 +/- 0.15, which was slightly more neutral than the bile added with EDTA.4Na alone. 4) In dogs, the bile duct was dilated and irrigated with an EDTA solution for 4 weeks. This irrigation resulted in no abnormalities in hepatic function, the serum calcium level, or the histology of the gallbladder or the common bile duct. In conclusion, the combined solution of EDTA.4Na-Urso-heparin is effective for the dissolution and disintegration of calcium bilirubinate stones with little disturbance of human bodies.
D000818:Animals; D001646:Bile; D001652:Bile Ducts; D001663:Bilirubin / Q000378:metabolism*; D002769:Cholelithiasis / Q000378:metabolism / Q000628:therapy*; D004285:Dogs; D004492:Edetic Acid / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D007507:Therapeutic Irrigation; D014580:Ursodeoxycholic Acid / Q000494:pharmacology
D014580:Ursodeoxycholic Acid; D006493:Heparin; D004492:Edetic Acid; D001663:Bilirubin