The effect of ethacrynic acid on biliary excretion, concentration of ioglycamide and bile flow in dogs.
The effect of ethacrynic acid (EA), an agent which increases bile acid independent bile flow, on the biliary excretion and concentration intravenously administered biliary contrast agent ioglycamide was studied on cholecystectomized anesthetized dogs equipped with Thomas cannula through which the common bile duct could be cannulated. One hour after cannulation i.v. infusion of ioglycamide at a rate of 4 mu mol./min./kg. was started. Two hours later 1 mg./kg. of ethacrynic acid was injected intravenously. This procedure was repeated three times on four dogs, five dogs receiving comparable volume of saline at comparable time serving as controls. Bile was collected at 15 min. intervals and simultaneous i.v. blood samples were taken. EA injection caused decrease in biliary ioglycamide concentration and biliary excretion of ioglycamide and a slight but statistically not significant decrease in bile flow. Since EA is an organic anion and the increase in bile flow induced by it has been shown to be related to the biliary excretion of its metabolites, it may be suggested that EA as an organic anion inhibited biliary excretion and hepatic uptake of ioglycamide but having lower choleretic effect its excretion could not increase the bile flow.
D000818:Animals; D001646:Bile / Q000378:metabolism*; D002758:Cholangiography; D003287:Contrast Media; D004285:Dogs; D004976:Ethacrynic Acid / Q000494:pharmacology*; D007463:Iodobenzoates / Q000097:blood*; D007471:Ioglycamic Acid / Q000097:blood*; D012636:Secretory Rate / Q000187:drug effects
D003287:Contrast Media; D007463:Iodobenzoates; D007471:Ioglycamic Acid; D004976:Ethacrynic Acid