[Detection of transduction by virulent bacteriophage phi KZ of Pseudomonas aeruginosa chromosomal markers in the presence of plasmid RMS148].
A virulent phage of Pseudomonas aeruginosa, phi KZ which is able to package DNA of 210 MD is a general transducer. The transducing activity of phi KZ can be revealed when using recipient bacteria containing RMS148 plasmid (the P7 group of incompatibility). This plasmid blocks the development of phi KZ and prevents the phage to kill bacteria. General transduction mediated by phi KZ may be useful for the primary localization of genetic markers in P. aeruginosa.
D001435:Bacteriophages / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000648:ultrastructure*; D004279:DNA, Viral / Q000235:genetics; D005819:Genetic Markers; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000648:ultrastructure; D014161:Transduction, Genetic; D014774:Virulence
D004279:DNA, Viral; D005819:Genetic Markers
Vyiavlenie transduktsii virulentnym bakteriofagom phi KZ kromosomal'-nykh markerov Pseudomonas aeruginosa v prisutstvii plazmidy RMS148.