Presence of an intrastrain cross-reactive idiotype on A/J antibodies of the IgE class specific for the p-azophenylarsonate group.
An intrastrain cross-reactive idiotype, CRIA, is associated with a large proportion of the anti-p-azophenylarsonate (anti-Ar) antibodies of A/J mice. The present experiments, in which the methods of direct and reverse passive cutaneous anaphylaxis (RPCA) were used, indicate that IgE anti-Ar antibodies are produced in A/J mice upon stimulation with a protein-Ar conjugate and that a large proportion of these antibodies express CRIA. The use of monoclonal anti-CRIA for RPCA eliminated the strong nonspecific reactions previously observed. The results provide a basis for studying factors that regulate the switch to IgE biosynthesis during an immune response.
D000818:Animals; D001391:Azo Compounds / Q000276:immunology*; D003429:Cross Reactions; D007073:Immunoglobulin E; D007130:Immunoglobulin Idiotypes / Q000032:analysis* / Q000235:genetics; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D010323:Passive Cutaneous Anaphylaxis; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D010132:p-Azobenzenearsonate / Q000276:immunology*
D001391:Azo Compounds; D007130:Immunoglobulin Idiotypes; D007073:Immunoglobulin E; D010132:p-Azobenzenearsonate