title: Localization of tRNA genes of Drosophila melanogaster by in situ hybridization.
abstract: Six purified tRNAs labeled with 125I by chemical or enzymatic methods were hybridized to polytene chromosomes of Drosophila melanogaster. The main chromosomal regions of hybridization wer: tRNAGly(GGA), 58A, 84C, and 90E; tRNALeu(2), 44E, 66B5-8, and 79F; tRNASer(2b), 86A, 88A9-12, and 94A6-8; tRNAThr(3), 47F and 87B; tRNAThr(4), 93A1-2; and tRNATyr(1 gamma), 19F, 22F-23A, 41, 50C1-4 and 85A. At 50C the hybridization of tRNATyr(1 gamma) was polymorphic in the giant strains. When the hybridization of three valine isoacceptors studied previously was re-investigated, it was found that only one hybridization site, 90BC, was shared between tRNAVal(3b) and tRNAVal(4). tRNAVal(3a) did not have any sites in common with the other two.
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics; D005796:Genes; D007814:Larva; D009154:Mutation; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D012343:RNA, Transfer / Q000235:genetics*; D012469:Salivary Glands / Q000166:cytology
chemical_list: D012343:RNA, Transfer