title: Repair of gamma-ray induced single-strand breaks in human lymphocytes.
abstract: Human peripheral blood lymphocytes are one of the most radiosensitive mammalian cells. We tested whether this radiosensitivity was associated with the persistance of unrepaired DNA damage in gamma-irradiated lymphocytes. Repair was determined by measuring the loss and recovery of DNA supercoiling when nucleoids are centrifuged in the presence of increasing amounts of ethidium bromide. Results show a limited repair capacity in resting human lymphocytes after gamma-irradiation.
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D004996:Ethidium / Q000494:pharmacology; D005260:Female; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects*; D010053:Ovary
chemical_list: D004277:DNA, Single-Stranded; D004996:Ethidium