In vitro translation of messenger RNA following exposure of human colon carcinoma cells in culture to 5-fluorouracil and 5-fluorouridine.
Total mRNA from human colon carcinoma cell line HT-29 treated with either 5-fluorouracil or 5-fluorouridine was assessed in vitro in a reticulocyte lysate translation system. Under conditions of known drug-induced cell lethality, fluoropyrimidine-modified mRNA did not show major quantitative or qualitative changes in translational activity. These results suggest that drug-modified mRNA is probably not associated with the cytotoxicity manifested by these drugs.
D002460:Cell Line; D002499:Centrifugation, Density Gradient; D003110:Colonic Neoplasms / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005472:Fluorouracil / Q000494:pharmacology*; D006801:Humans; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D014529:Uridine / Q000031:analogs & derivatives* / Q000494:pharmacology
D012333:RNA, Messenger; C001943:5-fluorouridine; D005472:Fluorouracil; D014529:Uridine