Regression of autophagic vacuoles in seminal vesicle cells following cycloheximide treatment.
A 0.25% fraction of cytoplasm was present in the autophagic vacuole compartment of the seminal vesicle cells of fasted and estrogen-treated mice. Autophagic vacuoles regressed rapidly after administration of 0.2 mg/g body weight cycloheximide to the animals and the decay appeared to follow first-order kinetics (average half-life 5.8 min, rate constant of regression 0.120 min-1). Our observations suggest that the drug suppressed the formation of autophagosomes. The fraction of cytoplasm removed by autophagy from the cells was estimated to be about 1.8% h under steady-state conditions.
D000818:Animals; D001343:Autophagy; D003513:Cycloheximide / Q000494:pharmacology*; D006207:Half-Life; D008297:Male; D051379:Mice; D009940:Organoids / Q000187:drug effects*; D010587:Phagocytosis; D012669:Seminal Vesicles / Q000502:physiology / Q000648:ultrastructure*; D014617:Vacuoles / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure
D003513:Cycloheximide