[Preservation of an isolated kidney in normothermic conditions by perfluorotributylamine emulsion perfusion].
Perfluorotributylamine emulsion (PFE) was used to preserve an isolated dog kidney under normothermal conditions with the aid of perfusion. The latter was carried out for 4 hours with the use of a pulse pump. The protein-saline solution (PSS) - 5% donor's albumin in Hanks solution, was used for reference purposes. During PFE perfusion, oxygen consumption by the kidney was noticed to be increased at the expense of a rise in the arterio-venous difference. Glucose concentration in the perfusate was unchanged during PFE perfusion, while that in PSS declined. Lactate dehydrogenase activity in PFE was noticeably lower than in PSS. During PFE perfusion, the concentration capacity of the kidney was 3.5 times higher as regards creatinine and 7.5 higher as regards potassium. It has been demonstrated that the 4-hour normothermal renal perfusion with 20 v% PFE enables preservation of the renal function.
D000818:Animals; D003470:Culture Media; D004285:Dogs; D004655:Emulsions; D005466:Fluorocarbons; D005947:Glucose / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D009926:Organ Preservation / Q000379:methods*; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption; D010477:Perfusion; D013696:Temperature; D014021:Tissue Preservation / Q000379:methods*
D003470:Culture Media; D004655:Emulsions; D005466:Fluorocarbons; C008807:perfluorotributylamine; D005947:Glucose; D010100:Oxygen
Sokhranenie izolirovannoĭ pochki v normotermicheskikh usloviiakh s pomosch'iu perfuzii émul'sieĭ ftortributilamina.