Antizyme release is an early event in ornithine decarboxylase induction by hair plucking.
Plucking of hair from the dorsal skin of rats resulted in a rapid decrease in ornithine decarboxylase (ODC) activity. A significant loss of activity did not occur in other skin enzymes under the same conditions and in vivo incorporation of [3H]-leucine in skin was not significantly decreased 60 min immediately following hair plucking. Treatment of ODC enzyme preparations with 10% (NH4)2SO4 resulted in recovery of approximately 75% greater ODC activity than in untreated samples, suggesting the presence of an inhibitor (antizyme). ODC inhibitor was detected in plucked skin; inhibitor levels increased after treatment of plucked skin extracts with 10% (NH4)2SO4.
D000818:Animals; D002262:Carboxy-Lyases / Q000096:biosynthesis*; D003513:Cycloheximide / Q000494:pharmacology; D004790:Enzyme Induction; D006197:Hair / Q000502:physiology*; D007700:Kinetics; D008297:Male; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D065108:Ornithine Decarboxylase Inhibitors; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000201:enzymology*
D065108:Ornithine Decarboxylase Inhibitors; D003513:Cycloheximide; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase