Solid-phase synthesis of thymosin beta 4: chemical and biological characterization of the synthetic peptide.
The chemical synthesis of thymosin beta 4 using a solid-phase procedure has been accomplished. The synthetic product was found to be homogeneous on paper electrophoresis at pH 6.5, high-performance liquid chromatography on a reversed-phase column, and isoelectric focusing using polyacrylamide gels. The synthetic material was also shown to be identical with the natural thymosin beta 4 by tryptic peptide mapping, amino acid compositional analyses, and polyacrylamide gel isoelectric focusing. Biologically, synthetic thymosin beta 4 was found to be as active as the natural compound in a terminal deoxynucleotidyltransferase induction assay and in a macrophage migration inhibition assay. The proposed structure of the peptide hormone was thus confirmed by a chemical synthesis.
D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D007525:Isoelectric Focusing; D008264:Macrophages / Q000187:drug effects / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010446:Peptide Fragments / Q000032:analysis; D013329:Structure-Activity Relationship; D013947:Thymosin / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000494:pharmacology; D013951:Thymus Hormones / Q000138:chemical synthesis*; D000099201:Thymosin beta(4)
D007202:Indicators and Reagents; D010446:Peptide Fragments; D013947:Thymosin; D013951:Thymus Hormones; D000099201:Thymosin beta(4)