Proportion of glycosylated hemoglobin in erythrocytes of a person with hemoglobins A, S, and G Philadelphia.
The apparent proportion of column-chromatographically measured glycosylated hemoglobin in erythrocytes from an individual with hemoglobins A, S, and G was only 2.3% because the slow glycosylated variant hemoglobins were retarded in the column. In contrast, the value for glycosylated hemoglobin was 7.8% by a new cellulose acetate electrophoretic method that includes use of dextran sulfate buffer. The erythrocyte metalloprotein, carbonic anhydrase B, was shown to co-migrate with glycosylated hemoglobin by this technique, however. Thus carbonic anhydrase B, HbF, and HbA all have weak attraction for negative charges at acid pH. We believe that carbonic anhydrase B should contribute significantly (at least 10-20% absorbance) to the HbA1 by this electrophoretic method. We conclude that microcolumn chromatography should remain the method of choice for HbA1 determination, that subject-based reference intervals should be used for HbS or HbC heterozygotes, and that electrophoretic HbA1 methods should be reserved for use with both HbS and HbC homozygotes and HbSC disease.
D000328:Adult; D001797:Blood Protein Electrophoresis; D003920:Diabetes Mellitus / Q000097:blood; D004588:Electrophoresis, Cellulose Acetate; D004912:Erythrocytes / Q000032:analysis*; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis*; D006451:Hemoglobin, Sickle / Q000031:analogs & derivatives* / Q000032:analysis; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D011247:Pregnancy
D006442:Glycated Hemoglobin; D006451:Hemoglobin, Sickle; D006455:Hemoglobins, Abnormal; C024241:glycated hemoglobin S; C031679:hemoglobin G Philadelphia