Use of monoclonal anti-rat IgE in a radioimmunoassay for antigen specific rat IgE.
A monoclonal mouse anti-rat IgE, ELIVB5 (B5) was used in a solid-phase radioimmunoassay (RIA) to detect antigen specific IgE in antisera from rats infected with a larval nematode. Binding of 125I-labeled B5 was specific for rat IgE and did not crossreact with human IgE. The RIA was used to demonstrate crossreactivity between antigens of closely related larval nematodes.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology*; D009348:Nematoda / Q000276:immunology*; D009349:Nematode Infections / Q000175:diagnosis / Q000276:immunology*; D011863:Radioimmunoassay; D051381:Rats
D000911:Antibodies, Monoclonal; D007073:Immunoglobulin E