Preferential alkaline phosphatase isoenzyme induction by sodium butyrate.
SW-620, a continuous cell line derived from a poorly differentiated human colon carcinoma, produces two alkaline phosphatases. Under basal conditions the heat-stable, term-placental is the major isoenzyme and the heat-labile, liver/bone/kidney form represents a minor component. Exposing SW-620 cells to sodium butyrate causes induction of increased levels of activity accompanied by a striking shift in isoenzyme distribution not observed heretofore. The activity increase is accounted for entirely by augmentation of the liver/bone/kidney isoenzyme, with the term-placental form not being affected. Two other known alkaline phosphatase inducers, prednisolone and hyperosmolality, do not influence specific activity and isoenzyme distribution. The preferential induction of the liver/bone/kidney form of alkaline phosphatase in SW-620 cells may reflect a butyrate-elicited expression of a more differentiated state.
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002460:Cell Line; D002999:Clone Cells / Q000201:enzymology; D003110:Colonic Neoplasms / Q000201:enzymology; D004790:Enzyme Induction; D006358:Hot Temperature; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis*
D002087:Butyrates; D007527:Isoenzymes; D020148:Butyric Acid; D000469:Alkaline Phosphatase