Inhibition of DNA synthesis does not influence the H1 histone synthesis in Tetrahymena pyriformis.
In the protozoan Tetrahymena pyriformis the DNA synthesis is stopped immediately and completely after addition of one of the two DNA synthesis inhibitors methotrexate + uridine and hydroxyurea to a cell suspension. However, the present experiments show, that the accumulation of labeled H1 histone in the inhibited cells is almost totally unaffected for more than two-thirds of a cell cycle after addition of either inhibitor.
D000818:Animals; D004261:DNA Replication / Q000187:drug effects*; D006657:Histones / Q000235:genetics*; D006918:Hydroxyurea / Q000494:pharmacology*; D007700:Kinetics; D008727:Methotrexate / Q000494:pharmacology*; D013769:Tetrahymena pyriformis / Q000187:drug effects / Q000378:metabolism*; D014529:Uridine / Q000494:pharmacology
D006657:Histones; D014529:Uridine; D006918:Hydroxyurea; D008727:Methotrexate