Effect of antioxidants on the proliferative response of canine lymphocytes in serum from dogs with vitamin E deficiency.
The in vitro effect of vitamin E and 3 other antioxidants--ethoxyquin, 2-mercaptoethanol, and ascorbic acid--on proliferation of canine lymphocytes was examined. Lymphocytes from 2 groups of dogs given a vitamin E-deficient diet or whelped from a bitch fed such a diet were cultured with pooled samples of serum from dogs fed a vitamin E-deficient diet or whelped from a bitch fed such a diet, or normal canine serum, and stimulated with phytohemagglutinin. Added vitamin E enhanced the responsiveness in serum from the dogs with vitamin E deficiency, but not in normal canine serum. A similar effect was noted with added ethoxyquin and 2-mercaptoethanol. Ascorbic acid had no effect on proliferation in either serum pool. These results indicated that depressed lymphocyte responsiveness seen with serum from vitamin E-deficient dogs may, at least in part, be due to a loss of antioxidant activity in this serum.
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D004283:Dog Diseases / Q000097:blood* / Q000276:immunology; D004285:Dogs; D005015:Ethoxyquin / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008623:Mercaptoethanol / Q000494:pharmacology; D014811:Vitamin E Deficiency / Q000097:blood / Q000276:immunology / Q000662:veterinary*
D000975:Antioxidants; D008623:Mercaptoethanol; D005015:Ethoxyquin; D001205:Ascorbic Acid