Sequence determination for N-linked oligosaccharides through the use of the nuclear Overhauser effect.
A nuclear magnetic resonance technique, the nuclear Overhauser effect, has been used to confirm the presence of Man alpha 1-2, GlcNAc beta 1-2, and Man alpha 1-3 residues and to establish the branching pattern and sequences in four classes of N-linked oligosaccharides. This method offers a rapid and nondestructive approach to the elucidation of sequences in carbohydrate chains, in contrast to enzymatic and chemical methods.
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006020:Glycopeptides; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009844:Oligosaccharides
D006020:Glycopeptides; D009844:Oligosaccharides