Isolation of human basophils by flow microfluorometry.
Human mononuclear cells labeled with fluorescein-conjugated anti-IgE antibody were subjected to flow microfluorometric analysis. Basophils were enriched to 97-99% purity with a 2-step cell sorting procedure. Trypan blue exclusion of sorted cells exceeded 90% and the net yield of the procedure was 15%.
D001491:Basophils; D002469:Cell Separation / Q000379:methods; D005434:Flow Cytometry; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis
D011947:Receptors, Antigen, B-Cell; D007073:Immunoglobulin E