Continuous culture of human/mouse lymphoid hybridomas: pattern of immunoglobulin secretion.
Fusion of human and mouse cells leads to preferential loss of human chromosomes. In this study the loss of human immunoglobulin heavy and light chain production by human/mouse lymphoid hybridomas has been followed longitudinally. A sensitive quantitative ELISA method has been used for measuring human immunoglobulins. The pattern of loss of heavy and light chain production suggests that the genes for human heavy and light chains are on separate chromosomes.
D000818:Animals; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas / Q000378:metabolism*; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis; D007146:Immunoglobulin lambda-Chains / Q000378:metabolism; D007148:Immunoglobulin mu-Chains / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism*; D008221:Lymphoid Tissue / Q000166:cytology*; D051379:Mice
D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007146:Immunoglobulin lambda-Chains; D007148:Immunoglobulin mu-Chains; D007136:Immunoglobulins