title: A method to evaluate the biosynthesis of glycosaminoglycans by the aorta of Cynomolgus monkey.
abstract: The biosynthesis of the glycosaminoglycans (GAGs) was investigated in vitro in the aortic tissue of the Cynomolgus monkey incubated with [14C]glucosamine. With the use of a new micromethod, it was possible to quantify the glycosaminoglycans and their radioactive distribution in the aortic tissue and incubation medium. Labeled and nonlabeled chondroitin 6-sulfate, dermatan sulfate, heparan sulfate and hyaluronic acid were measured. Since the sensitivity of this procedure is between 5 and 20 micrograms of GAGs, as little as 5 mg of dry defatted aortic tissue is sufficient for chemical and radioactive analyses.
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism*; D002809:Chondroitin Sulfates / Q000096:biosynthesis; D003871:Dermatan Sulfate / Q000096:biosynthesis; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D008252:Macaca fascicularis; D008297:Male
chemical_list: D006025:Glycosaminoglycans; D003871:Dermatan Sulfate; D002809:Chondroitin Sulfates; D005944:Glucosamine