Increased uptake of actinomycin D in Ehrlich ascites tumour cells induced by daunorubicin.
The influence of anthracyclines on the uptake of actinomycin D in Ehrlich ascites tumour cells was studied in vitro. The anthracycline daunorubicin significantly increased the [3H]actinomycin D uptake both in whole tumour cells and in isolated nuclei of tumour cells. On the other hand, actinomycin D increased daunorubicin uptake only to a very small degree. Experiments with other intercalating drugs did not influence [3H]actinomycin D binding, suggesting a specific interaction between actinomycin D and the anthracyclines. The mechanism behind this interference is discussed. The increased cellular uptake of actinomycin D in the presence of daunorubicin may have implications for antineoplastic combination chemotherapy.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D003609:Dactinomycin / Q000378:metabolism*; D003630:Daunorubicin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004359:Drug Therapy, Combination; D051379:Mice; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000378:metabolism
D003609:Dactinomycin; D003630:Daunorubicin