PCB isomers and congeners: induction of aryl hydrocarbon hydroxylase and ethoxyresorufin O-deethylase enzyme activities in rat hepatoma cells.
The effects of structure on the activity of 15 polychlorinated biphenyl (PCB) isomers and congeners as inducers of cytochrome P-448-dependent monooxygenases in rat hepatoma cell cultures was investigated. All the PCBs which have previously been classified as aryl hydrocarbon hydroxylase (AHH) inducers in the immature male Wistar rat also induced benzo[a]pyrene hydroxylase and ethoxyresorufin (ER) O-deethylase activity in the rat hepatoma H-4-II-E cells. The relative activities of the compounds were evaluated by comparing the doses required to half-maximally induce the two enzyme activities. The most potent PCB inducer, 3,3',4,4',5-pentachlorobiphenyl, exhibited molar EC50 values of 2.48 X 10(-10) and 2.40 X 10(-10) for the induction of ER O-deethylase and benzo[a]pyrene hydroxylase enzyme activities, respectively. These values were only 3- to 4-fold lower than the data obtained for the highly toxic 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The relative activities of the PCB congeners as inducers of cytochrome P-448-dependent monooxygenase in vitro were similar to their potencies as microsomal enzyme inducers in the immature male rat.
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis*; D002478:Cells, Cultured; D019363:Cytochrome P-450 CYP1A1; D004790:Enzyme Induction / Q000187:drug effects; D007536:Isomerism; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D010088:Oxidoreductases / Q000096:biosynthesis*; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D051381:Rats
D011078:Polychlorinated Biphenyls; D010088:Oxidoreductases; D001189:Aryl Hydrocarbon Hydroxylases; D019363:Cytochrome P-450 CYP1A1