title: Effects of indomethacin on contractility of isolated human umbilical artery.
abstract: Contractions of isolated strips of human umbilical artery (HUA) induced by arachidonic acid were antagonized by indomethacin at concentrations as low as 1 microgram/ml. Much higher concentrations (25 and 65 microgram/ml) were needed to relax KCl-contracted strips of HUA. These data suggest that indomethacin at 1 microgram/ml inhibits endogenous synthesis of vasoconstrictive prostaglandin-like substances whereas indomethacin at higher concentrations causes nonspecific relaxation. Contractions induced by 5-hydroxytryptamine (5-HT), KCl and prostaglandin F2 alpha (PGF2 alpha) were antagonized by indomethacin at 25 and 65 microgram/ml, but not at 2.5 microgram/ml. These data suggest that the contractile responses to 5-HT, KCl and PGF2 alpha are not mediated by endogenous prostaglandin-like substances. Contractions induced by adenosine triphosphate (ATP), in contrast, were completely inhibited by indomethacin (1 microgram/ml). Therefore, the data suggest that prostaglandin-like substances may mediate contractions to ATP.
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D015237:Dinoprost; D004347:Drug Interactions; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology*; D009119:Muscle Contraction / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D011189:Potassium Chloride / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology; D014469:Umbilical Arteries / Q000187:drug effects
chemical_list: D001095:Arachidonic Acids; D011460:Prostaglandins F; D016718:Arachidonic Acid; D012701:Serotonin; D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D015237:Dinoprost; D007213:Indomethacin