Effect of N,alpha-tosyl-L-phenylalanine chloromethyl ketone on measles virus P and M polypeptides.
Treatment of measles virus-infected cells with 1 mM N,alpha-tosyl-L-phenylalanine chloromethyl ketone (TPCK) was observed to change the polyacrylamide gel migration of viral polypeptides P and M. Untreated cells contained P as a mixture of P1 (70,000 daltons) and P2 (65,000 daltons) and M as a 38,000-dalton band M1 and a slightly smaller band, M2. TPCK treatment resulted in conversion of P largely to the 65,000-dalton band and of M to M1 and a slightly slower-migrating band. This effect could also be demonstrated by treating homogenates of infected cells with TPCK, and the evidence suggests that the compound reacts directly with the viral P and M polypeptides and thereby changes their gel migration. TPCK also inhibited measles virus-associated protein kinase, and treatment of virion preparations with the compound resulted in a loss of infectivity; however, it was not possible to directly correlate the inhibitory effect on these two biological functions with the change seen in polypeptides P and M.
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D008459:Measles virus / Q000187:drug effects* / Q000254:growth & development; D008970:Molecular Weight; D011480:Protease Inhibitors; D047428:Protein Kinase Inhibitors; D014108:Tosylphenylalanyl Chloromethyl Ketone / Q000494:pharmacology*; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000096:biosynthesis
D000590:Amino Acid Chloromethyl Ketones; D011480:Protease Inhibitors; D047428:Protein Kinase Inhibitors; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D014108:Tosylphenylalanyl Chloromethyl Ketone