Production of two lymphocyte chemotactic factors by antigen-stimulated guinea pig lymphocytes.
Lymph node cells from guinea pigs sensitized by BCG elaborated two chemotactic factors for lymphocytes when stimulated with purified protein derivative in vitro 4 weeks after immunization. Production of the factors occurred within 24 h of incubation. The two factors were termed alpha- and beta-lymphocyte chemotactic factors (alpha-LCF, beta-LCF), relative to their elution order on gel chromatography. The molecular weights were about 160,000 and 27,000, respectively. alpha-LCF was labile to heating at 56 degrees C for 30 min. Both factors were sensitive to digestion by trypsin, and were effective for T cells but not for B cells.
D000818:Animals; D000941:Antigens / Q000276:immunology*; D001500:BCG Vaccine / Q000008:administration & dosage; D002448:Cell Adhesion; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D006168:Guinea Pigs; D007700:Kinetics; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000145:classification / Q000276:immunology*; D008222:Lymphokines / Q000032:analysis / Q000096:biosynthesis* / Q000494:pharmacology; D008297:Male; D012795:Sialoglycoproteins / Q000032:analysis / Q000096:biosynthesis* / Q000494:pharmacology; D014373:Tuberculin / Q000276:immunology
D000941:Antigens; D001500:BCG Vaccine; D008222:Lymphokines; D012795:Sialoglycoproteins; D014373:Tuberculin; C018997:lymphotactin; D003208:Concanavalin A