title: Tubulin genes are tandemly linked and clustered in the genome of trypanosoma brucei.
abstract: We have isolated cDNA and genomic clones containing alpha- and beta-tubulin genes from Trypanosoma brucei. Each clone has been mapped, and the identity of the tubulin genes has been established by cross-hybridization with cloned chicken tubulin genes and by hybridization-selection and translation of trypanosome tubulin mRNA. In contrast with the dispersed organization of tubulin genes in other organisms, trypanosome alpha- and beta-tubulin genes are physically linked and clustered in tandem repeats of approximately 13-17 copies per haploid genome of alternating alpha- and beta-tubulin sequences.
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005796:Genes; D008040:Genetic Linkage; D012333:RNA, Messenger / Q000235:genetics; D014346:Trypanosoma brucei brucei / Q000235:genetics*; D014404:Tubulin / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D014404:Tubulin