Radioimmunoassays for two types of human urinary ribonucleases: differential determination of ribonucleases in human serum.
We developed radioimmunoassays for two types of human urinary ribonucleases. The assays are sensitive, reproducible and specific. One human urinary ribonuclease (RNase UL) showed strong immunological identity with human pancreatic ribonuclease, and the other ribonuclease (RNase Us) showed strong immuno cross-reactivity with human liver ribonuclease. There was no immuno cross-reactivity between these two urinary ribonucleases. Serum immunoreactive RNase UL was eluted as four peaks on phosphocellulose chromatography, whereas immunoreactive RNase US was eluted as a single peak. Serum content of immunoreactive RNase UL was 354 +/- 105 ng/ml (mean +/- SD) in normal individuals, and that of immunoreactive RNase Us was 15.9 +/- 5.7 ng/ml (mean +/- SD). No correlation was demonstrated between these two ribonuclease contents in serum.
D000328:Adult; D000368:Aged; D000918:Antibody Specificity; D003429:Cross Reactions; D005260:Female; D006801:Humans; D008099:Liver / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D010179:Pancreas / Q000201:enzymology; D011863:Radioimmunoassay / Q000379:methods; D012260:Ribonucleases / Q000032:analysis* / Q000097:blood / Q000652:urine
D012260:Ribonucleases