[3H]spiperone binding sites in rat primary glial cultures, C6 glioma, and B104 neuroblastoma.
Binding sites for [3H]spiperone were detected on membranes from primary glial cultures from neonatal rat cortex and striatum and from the C6 glioma cells. [3H]Spiperone binding was displaceable by d-butaclamol. However, competition studies suggest that [3H]spiperone binding to primary glial cultures was mainly to serotonergic sites, and binding to the C6 glioma was alpha-adrenergic. No specific binding was detected to membranes from B104 neuroblastoma cells. Although [3H]spiperone binding to glial sites on whole striatum under generally used conditions is small (about 10%), the striatal glial hyperactivity that is often associated with neuronal degeneration could lead to an overestimation of presumed neuronal binding sites for dopaminergic ligands.
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002090:Butyrophenones / Q000378:metabolism*; D002460:Cell Line; D002540:Cerebral Cortex / Q000166:cytology; D003342:Corpus Striatum / Q000166:cytology; D004298:Dopamine / Q000378:metabolism; D005910:Glioma / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism*; D051381:Rats; D012701:Serotonin / Q000378:metabolism; D013134:Spiperone / Q000378:metabolism*
D002090:Butyrophenones; D012701:Serotonin; D013134:Spiperone; D004298:Dopamine