Effects of passive immunization against oestradiol on mouse ovum transport.
The ability of anti-oestradiol immunoglobulin to withhold oestradiol-17 beta from its target tissue was examined. The total oestradiol-17 beta binding capacity present in in-vitro incubations or injected into mice intravenously was related to the amount of [3H]oestradiol present in the media or intravenously injected into the animals respectively. When the ratio of binding capacity to [3H]oestradiol was above 74:1, [3H]oestradiol was successfully withheld from uterine tissue in vitro and in vivo. Injecting anti-oestradiol immunoglobulin into mice before administration of a tube-locking dose of oestradiol-17 beta ensured normal passage of ova through the oviduct. Daily administration of anti-oestradiol immunoglobulin to PMSG-hCG stimulated mice (starting 72 h before hCG injection) induced retention of ova for at least 2 days beyond the time when all ova had left the oviducts of control animals. The binding capacity: oestradiol-17 beta concentration ratios of sera from these animals were greater than 250:1 throughout the experimental period. Non-specific immunoglobulin had no such effects, indicating the specificity of the anti-oestradiol immunoglobulin response.
D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004958:Estradiol / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology; D007116:Immunization, Passive; D007136:Immunoglobulins / Q000276:immunology; D051379:Mice; D010066:Ovum Transport; D011374:Progesterone / Q000097:blood; D013997:Time Factors; D014599:Uterus / Q000378:metabolism
D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D007136:Immunoglobulins; D011374:Progesterone; D004958:Estradiol