title: Proton electrochemical gradients in washed cells of Nitrosomonas europaea and Nitrobacter agilis.
abstract: The components of the proton motive force (Deltap), namely, membrane potential (Deltapsi) and transmembrane pH gradient (DeltapH), were determined in the nitrifying bacteria Nitrosomonas europaea and Nitrobacter agilis. In these bacteria both Deltapsi and DeltapH were dependent on external pH. Thus at pH 8.0, Nitrosomonas europaea and Nitrobacter agilis had Deltapsi values of 173 mV and 125 mV (inside negative), respectively, as determined by the distribution of the lipophilic cation [(3)H]tetraphenyl phosphonium. Intracellular pH was determined by the distribution of two weak acids, (14)C-benzoic and (14)C-acetyl salicylic, and the weak base [(14)C]methylamine. Nitrosomonas europaea accumulated (14)C-benzoic acid and (14)C-acetyl salicylic acid when the external pH was below 7.0 and [(14)C]methylamine at alkaline pH. Similarly, Nitrobacter agilis accumulated the two weak acids below an external pH of about 7.5 and [(14)C]methylamine above this pH. As these bacteria grow best between pH 7.5 and 8.0, they do not appear to have a DeltapH (inside alkaline). Thus, above pH 7.0 for Nitrosomonas europaea and pH 7.5 for Nitrobacter agilis, Deltapsi only contributed to Deltap. In Nitrosomonas europaea the total Deltap remained almost constant (145 to 135 mV) when the external pH was varied from 6 to 8.5. In Nitrobacter agilis, Deltap decreased from 178 mV (inside negative) at pH 6.0 to 95 mV at pH 8.5. Intracellular pH in Nitrosomonas europaea varied from 6.3 at an external pH of 6.0 to 7.8 at external pH 8.5. In Nitrobacter agilis, however, intracellular pH was relatively constant (7.3 to 7.8) over an external pH range of 6 to 8.5. In Nitrosomonas europaea, Deltap and its components (Deltapsi and DeltapH) remained constant in cells at various stages of growth, so that the metabolic state of cells did not affect Deltap. Such an experiment was not possible with Nitrobacter agilis because of low cell yields. The effects of protonophores and ATPase inhibitors on DeltapH and Deltapsi in the two nitrifying bacteria are considered.
mesh_terms: D006863:Hydrogen-Ion Concentration; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D009576:Nitrobacter / Q000378:metabolism*; D009606:Nitrosomonas / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism / Q000494:pharmacology; D012977:Sodium Nitrite / Q000378:metabolism / Q000494:pharmacology
chemical_list: D006898:Hydroxylamines; D000644:Quaternary Ammonium Compounds; D019811:Hydroxylamine; D012977:Sodium Nitrite