Large scale isolation of zonae pellucidae from ovarian oocytes of mice.
A large scale method for the isolation of zonae pellucidae (ZP) from mouse ovaries is described. It involves the squashing of the ovaries on a screen (70 X 70 M mesh) and sedimentation of the material in a discontinuous gradient. It is possible to obtain free ZP in useful quantities in about 1 h's work.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D005260:Female; D051379:Mice; D009865:Oocytes / Q000166:cytology*; D010063:Ovum / Q000166:cytology*; D013997:Time Factors; D015044:Zona Pellucida