Isolation of human IgA and IgM from normal serum using polyethylene glycol precipitation and affinity chromatography.
A simple method is described for the preparation of highly purified IgA and IgM from small volumes of human serum. Enriched IgM and IgA fractions were prepared by precipitation with 7% (w/v) and 14% (w/v) polyethylene glycol respectively. This was followed by affinity chromatography and gel filtration. The final recovery of both IgA and IgM was approximately 30%. The purified preparations obtained were characterized by immunoelectrophoresis, double immunodiffusion, radial immunodiffusion, radioimmunoassay and gel filtration.
D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D006801:Humans; D007070:Immunoglobulin A / Q000302:isolation & purification*; D007075:Immunoglobulin M / Q000302:isolation & purification*; D011092:Polyethylene Glycols
D007070:Immunoglobulin A; D007075:Immunoglobulin M; D011092:Polyethylene Glycols