A simple, rapid, high-resolution chromosome technic for lymphocytes.
Phytohemagglutinin (PHA) stimulated lymphocytes were exposed to a hypotonic solution consisting of equal parts of 0.075 M 2-Mercaptoethanol and 0.075 M KCl, followed by addition of colcemid. The cells were fixed and washed in the usual manner. The slides then were subjected to trypsin-Giemsa banding. This method yields a high percentage of mitotic figures in prophase and prometaphase, ideal for high-resolution chromosome analysis.
D006801:Humans; D007621:Karyotyping / Q000379:methods*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011418:Prophase
D010835:Phytohemagglutinins