Opal suppressor serine tRNAs from bovine liver form phosphoseryl-tRNA.
An unusual minor species of bovine liver serine tRNA has previously been isolated, sequenced, and found to suppress the UGA termination codon in protein synthesis in vitro [Diamond, A., Dudock, B. & Hatfield, D. (1981) Cell 25, 497-506]. We have now found that this tRNA can be a substrate in a specific phosphorylation reaction in which phosphoseryl-tRNA is formed. Moreover, bovine liver contains a second UGA suppressor serine tRNA (tRNASerNCA; N is a modified nucleoside) which also forms phosphoseryl-tRNA. The nucleotide sequence and coding properties of tRNASerNCA are presented.
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D008099:Liver / Q000502:physiology; D009690:Nucleic Acid Conformation; D010443:Peptide Chain Termination, Translational; D010768:Phosphoserine / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics*; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics*; D012694:Serine / Q000031:analogs & derivatives* / Q000235:genetics; D013489:Suppression, Genetic
D012346:RNA, Transfer, Amino Acyl; D010768:Phosphoserine; D012694:Serine; D012343:RNA, Transfer