Laser measurement of the motility of bull spermatozoa in an egg-yolk diluent.
An objective method of assessing bull sperm motility by specifying the mean swimming speed and number of motile spermatozoa in a sample is described. Laser light was conducted into diluted semen samples using a fibre-optic Doppler anemometer (FODA). The signal correlation of the back-scattered laser light was modelled using least squares computer curve fitting of the resulting data. Agreement was found between the mean swimming speeds obtained and those measured using time lapse photography.
D000818:Animals; D002417:Cattle / Q000502:physiology*; D007834:Lasers; D008297:Male; D008722:Methods; D012542:Scattering, Radiation; D013081:Sperm Motility; D013997:Time Factors