[Detection, using fluorescent lectins, of specific carbohydrate chains in primordial germ cells of anuran amphibians].
A study of primordial germ cells (PGC) of Amphibia Anura was carried out after treatment of sections by different fluorescein isothiocyanate conjugated lectins (FITC-lectins). Specific labelling on the PGC is obtained with lectins, the activity of which is inhibited by D-galactose or N-acetyl-galactosamine. These osidic groups appear to be located more specifically on the PGC. The same labelling pattern is not obtained with lectins possessing major affinity for mannose, glucose, fucose and N-acetyl-glucosamine. Furthermore, changes in labelling pattern are observed during migration of PGC. It is suggested that D-galactose and N-acetyl-galactosamine might be related to membrane activity of PGC during migration. Ultrastructural study of the visualization of cell surface carbohydrates supplies some information on the localisation of these lectins receptors.
D000818:Animals; D002025:Bufonidae; D004625:Embryo, Nonmammalian / Q000166:cytology* / Q000648:ultrastructure; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005854:Germ Cells / Q000166:cytology*; D037102:Lectins; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D011898:Ranidae; D013861:Thiocyanates; D014981:Xenopus
D005452:Fluoresceins; D005456:Fluorescent Dyes; D037102:Lectins; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate
Détection au moyen de lectines fluorescentes de chaînes osidiques spécifiques des cellules germinales primordiales d'amphibiens anoures.