Solid-phase vitamin B12 assays using polyacrylamide-bound intrinsic factor and polyacrylamide-bound R-binder.
A new solid phase vitamin B12 assay is described using intrinsic factor to measure microbiologically-available B12 and R-binder to measure total B12. The solid phase reagent consists of intrinsic factor coupled to polyacrylamide beads and salivary R-binder coupled to polyacrylamide beads. The assay is simple to perform and separates completely sera from controls and patients with megaloblastic anaemia due to B12 deficiency.
D000180:Acrylic Resins; D000749:Anemia, Megaloblastic / Q000097:blood / Q000209:etiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007437:Intrinsic Factor; D008027:Light; D008722:Methods; D009687:Nuclear Proteins; D009698:Nucleoproteins; D009994:Osmolar Concentration; D013997:Time Factors; D014805:Vitamin B 12 / Q000097:blood*; D014806:Vitamin B 12 Deficiency / Q000150:complications; D000099166:Polyacrylamides
D000180:Acrylic Resins; D007437:Intrinsic Factor; D009687:Nuclear Proteins; D009698:Nucleoproteins; D014805:Vitamin B 12; D000099166:Polyacrylamides