Liver glycogen synthase and phosphorylase changes in vivo with hypoxia and anesthetics.
Methods for obtaining and processing rat liver for determination of glycogen phosphorylase a and synthase I activity were studied. An extremely rapid and profound increase in phosphorylase was induced by hypoxia. The effect on synthase I was slower and less striking. Using alpha- and beta-adrenergic antagonists, a catecholamine-depleting agent, and a ganglionic blocking agent, it was determined that adrenergic stimulation secondary to the surgical procedure required to obtain the liver was not a significant factor. The anesthetic agent used also had a significant effect on the proportion of phosphorylase in the a form. Seconal anesthesia resulted in lower phosphorylase a levels than did ether or urethan anesthesia.
D000758:Anesthesia; D000818:Animals; D006006:Glycogen Synthase / Q000378:metabolism; D000860:Hypoxia / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D010425:Pentolinium Tartrate / Q000494:pharmacology; D010643:Phenoxybenzamine / Q000494:pharmacology; D010646:Phentolamine / Q000494:pharmacology; D006005:Phosphorylases / Q000378:metabolism*; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012110:Reserpine / Q000494:pharmacology
D010643:Phenoxybenzamine; D012110:Reserpine; D010425:Pentolinium Tartrate; D011433:Propranolol; D006005:Phosphorylases; D006006:Glycogen Synthase; D010646:Phentolamine