title: Kinetic mechanism of bovine liver argininosuccinate lyase.
abstract: The kinetic mechanism of bovine liver argininosuccinate lyase has been determined at pH 7.5, 25 degrees C. Fumarate and arginine are both noncompetitive inhibitors versus argininosuccinate. The dead-end inhibitor, succinate, is competitive versus fumarate and argininosuccinate, but noncompetitive versus arginine. Citrulline is competitive versus arginine and noncompetitive versus argininosuccinate and fumarate. The results are consistent with a random mechanism with the formation of two dead-end complexes: E . argininosuccinate . fumarate and E . argininosuccinate . arginine. No evidence was obtained for nonlinear reciprocal plots. The equilibrium constant was found to be 3.7 mM.
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology; D001123:Argininosuccinate Lyase / Q000037:antagonists & inhibitors*; D002417:Cattle; D005650:Fumarates / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008190:Lyases / Q000037:antagonists & inhibitors*; D013379:Substrate Specificity
chemical_list: D005650:Fumarates; D001120:Arginine; D008190:Lyases; D001123:Argininosuccinate Lyase