Determination of mitomycin C in plasma, serum and urine by high-performance liquid chromatography with ultra-violet and electrochemical detection.
The performance of a number of normal phase and reversed-phase systems, with ultraviolet detection at 360 nm, has been investigated with respect to their applicability to pharmacokinetic studies of mitomycin C (MMC). The reversed-phase system developed was also combined with a polarographic detector in order to compare the sensitivity and selectivity of ultraviolet and electrochemical detection. A simple isolation procedure, based on the adsorption of MMC on a non-ionogenic resin, has been developed. The developed assay is applied to a pharmacokinetic study from which some examples are given.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D006801:Humans; D007700:Kinetics; D016685:Mitomycin; D008937:Mitomycins / Q000032:analysis* / Q000097:blood / Q000652:urine; D011048:Polarography; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D008937:Mitomycins; D016685:Mitomycin