[Histochemical determination of beta-hydroxybutyrate dehydrogenase in the brush border of the cortex epithelium of the rat kidney].
NAD-beta-hydroxybutyrate dehydrogenase activity was investigated in the kidneys of male and female adult Wistar rats using membrane incubating technique. In this connection the enzyme activity presents an other topochemical pattern as in comparison with conventional incubating technique. In the kidney cortex an additional reaction was demonstrated in the convoluted portion of proximal tubules. In connection with the results of biochemical investigations the soluble enzyme is characterized as a nonmitochondrial beta-hydroxybutyrate dehydrogenase of proximal kidney tubules (brush border membrane enzyme?).
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology*; D004587:Electrophoresis, Agar Gel; D004848:Epithelium / Q000201:enzymology; D005260:Female; D006651:Histocytochemistry; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism*; D007672:Kidney Cortex / Q000201:enzymology; D007687:Kidney Tubules, Proximal / Q000201:enzymology*; D008297:Male; D008871:Microvilli / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
D006884:Hydroxybutyrate Dehydrogenase
Zur histochemischen Erfassung einer beta-Hydroxybutyrat-Dehydrogenase im Bürstensaum der Hauptstückepithelien der Rattenniere.