Structure and location of asparagine-linked oligosaccharides in the Fc region of A human immunoglobulin D.
Seven kinds of asparagine-linked oligosaccharides were bound to the Fc region of a human immunoglobulin D(NIG-65). The oligosaccharides quantitatively released from four species of glycopeptides by digestion with almond glycopeptidase, were separated by Bio-Gel p-4 column chromatography and were purified further by thin-layer chromatography. The sugars were identified with GC-MS following the permethylation of respective oligosaccharide. To Asn-68 (NIG-65 Fc numbering (1)), two kinds of high-mannose-type oligosaccharides were bonded. To Asn-159, a kind of hybride-type and two kinds of bisected complex-type oligosaccharides were attached. From Asn-210, four kinds of bisected complex-type oligosaccharides were isolated.
D000818:Animals; D001216:Asparagine / Q000032:analysis*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002645:Chickens; D005260:Female; D005340:Fibrinogen; D006020:Glycopeptides / Q000302:isolation & purification*; D006801:Humans; D007072:Immunoglobulin D; D007141:Immunoglobulin Fc Fragments; D009844:Oligosaccharides / Q000032:analysis*; D010047:Ovalbumin; D011784:Quail; D013329:Structure-Activity Relationship
D006020:Glycopeptides; D007072:Immunoglobulin D; D007141:Immunoglobulin Fc Fragments; D009844:Oligosaccharides; D001216:Asparagine; D005340:Fibrinogen; D010047:Ovalbumin