Anti-pseudomonas immunoglobulin. II. Preparation of purified sheep immunoglobulins.
Several methods of fractionation of sheep anti-sera against Pseudomonas aeruginosa were applied for the preparation of immunoglobulins. The methods involving salting out with ammonium sulfate or purification by means of caprylic acid were shown to be most suitable for isolation of immunoglobulins under manufacturing conditions. The preparations obtained by these methods agglutinated Pseudomonas aeruginosa, suspension in vitro and protected mice against lethal infection with this microorganism.
D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D000918:Antibody Specificity; D011489:Protein Denaturation; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D012756:Sheep
D000907:Antibodies, Bacterial