title: Assay of prostacyclin synthase using [5,6-3H]PGH2.
mesh_terms: D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System; D011464:Epoprostenol / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D066298:In Vitro Techniques; D019746:Intramolecular Oxidoreductases; D007553:Isotope Labeling; D008297:Male; D008861:Microsomes / Q000378:metabolism; D011449:Prostaglandin Endoperoxides / Q000378:metabolism*; D011453:Prostaglandins / Q000096:biosynthesis*; D011463:Prostaglandins H / Q000378:metabolism*; D012669:Seminal Vesicles / Q000201:enzymology; D012756:Sheep; D013552:Swine; D014316:Tritium
chemical_list: D001095:Arachidonic Acids; D011449:Prostaglandin Endoperoxides; D011453:Prostaglandins; D011463:Prostaglandins H; D014316:Tritium; D016718:Arachidonic Acid; D003577:Cytochrome P-450 Enzyme System; D011464:Epoprostenol; D019746:Intramolecular Oxidoreductases; C022461:prostacyclin synthetase