Effects of cyclosporin A on the immune system of the mouse. I. Evidence for a direct selective effect of cyclosporin A on B cells responding to anti-immunoglobulin antibodies.
Previous in vivo studies have shown that the immunsuppressive peptide cyclosporin A (CsA) selectively suppresses antibody responses to nonmitogenic T-independent (TI-2) antigens, but not those to mitogenic (TI-1) antigens. This report demonstrates that in vitro CsA suppresses the polyclonal proliferative response of B cells to anti-Ig (anti-mu) antibodies at 300-400 fold lower doses than are required to inhibit B cell proliferation induced by lipopolysaccharide. Since the proliferative response to anti-mu requires neither T cells nor macrophages, it is concluded that CsA has a direct inhibitory effect on the B cells responding to this mitogen. The data support the concept that the B cells responding to TI-2 antigens are contained within the population which is stimulated polyclonally by anti-mu, and that lipopolysaccharide stimulates a distinct B cell subpopulation. They do not, however, exclude the possibility that anti-mu and lipopolysaccharide stimulate the same B cell population via two biochemically distinct triggering mechanisms, one of which is CsA-sensitive and the other CsA-resistant.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D001402:B-Lymphocytes / Q000187:drug effects*; D002448:Cell Adhesion; D003208:Concanavalin A / Q000494:pharmacology; D003524:Cyclosporins / Q000494:pharmacology*; D005260:Female; D007143:Immunoglobulin Heavy Chains / Q000276:immunology*; D007148:Immunoglobulin mu-Chains / Q000276:immunology*; D007166:Immunosuppressive Agents / Q000494:pharmacology; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D003524:Cyclosporins; D007143:Immunoglobulin Heavy Chains; D007148:Immunoglobulin mu-Chains; D007166:Immunosuppressive Agents; D008070:Lipopolysaccharides; D003208:Concanavalin A