title: Infectious bovine keratoconjunctivitis: bacteriologic, immunologic, and clinical responses of cattle to experimental exposure with Moraxella bovis.
abstract: The bacteriologic, immunologic, and clinical responses of 3- to 4-month old Holstein-Friesian calves to experimental exposure with Moraxella bovis type 10900 has been investigated. After u.v. radiation and intraconjunctival exposure with 1.9 X 10(7) microorganisms, each eye of 16 calves exhibited signs of blepharospasm, photophobia, and increased lacrimation. Bacteria were recovered from exposed eyes for 2-7 consecutive weeks before maximal clinical response occurred. The severity of the cases varied from eyes that exhibited mild signs to severe clinical cases with profuse lacrimation, conjunctival swelling, corneal opacity, and ulceration. By 70 days after exposure, M. bovis could not be recovered from any conjunctival swabs, and clinical signs were not observed. Four non-exposed control animals did not develop clinical signs nor was M. bovis recovered from conjunctival swabs. Lacrimal secretions collected at the time of and 1 week after maximal clinical response had significantly elevated levels of total protein as compared to those collected 3, 2, and 1 week before, and 2 and 3 weeks after maximal clinical response. A passive hemagglutination test, using tanned formalized sheep erythrocytes sensitized with M. bovis sonicate antigen, detected antibody in lacrimal secretions from 22 of 32 eyes. The appearance of specific antibody in lacrimal secretions correlated with the amelioration of clinical signs and the decline in numbers of M. bovis microorganisms recovered from conjunctival swabs.
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D001424:Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology*; D007637:Keratoconjunctivitis / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D009016:Moraxella / Q000276:immunology / Q000302:isolation & purification*; D013666:Tears / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial