An intracellular factor which affects erythroid differentiation in mouse Friend cells.
When permeabilized Friend cells, which had been briefly treated by DMSO, were exposed to cell-free extracts from UV irradiated cells, a small but significant number of the cells became reactive to benzidine, a characteristic of erythroid differentiation. The activity in the extracts was apparently induced following UV irradiation, reaching a maximum 25 to 30 h after irradiation. Although a similar activity was detected in the extract from mitomycin C treated cells, little activity was detected in the extract from cells treated with DMSO, a potent erythroid inducing agent. The induction of the active factor by UV irradiation was not specific of Friend cells since similar inducing activities were detected in the extract from non-erythroid murine cells irradiated by UV. The active factor in the extract seems to be a protein, judged from its heat sensitivity and high molecular weight. Significance of this finding in relation to cellular differentiation and DNA damage is discussed.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D007700:Kinetics; D007942:Leukemia, Experimental / Q000503:physiopathology*; D051379:Mice; D014466:Ultraviolet Rays
D004121:Dimethyl Sulfoxide