The effect of hypoxia on the synthesis of collagen and glycosaminoglycans by cultured pig aortic endothelium.
Porcine aortic endothelium cultured at 20% oxygen concentration synthesizes collagen and three of its marker enzymes - proline and lysine hydroxylases and lysine oxidase, the cross-link enzyme. It also synthesizes and secretes hyaluronic acid, dermatan sulphate, possibly heparan sulphate, large amounts of chondroitin 4-sulphate and smaller amounts of chondroitin 6-sulphate. Growth of these cells for 24 h in 0%, or 2% oxygen results in little change in cell numbers or cell protein but a fall in collagen synthesis and in proline and lysine hydroxylases, but a rise in lysine oxidase. There is a considerable increase in synthesis and secretion of all the glycosaminoglycans found. The cell lipids appear qualitatively unchanged. Apart from increased lysosomes seen at 0% oxygen, no ultrastructural changes appear to occur. These findings illustrate the lability of the endothelial response to oxygen lack.
D000818:Animals; D001011:Aorta / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000096:biosynthesis; D003094:Collagen / Q000096:biosynthesis*; D003871:Dermatan Sulfate / Q000096:biosynthesis; D004588:Electrophoresis, Cellulose Acetate; D004727:Endothelium / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D000860:Hypoxia / Q000378:metabolism*; D008055:Lipids / Q000032:analysis; D010100:Oxygen / Q000494:pharmacology; D013552:Swine
D006025:Glycosaminoglycans; D008055:Lipids; D003871:Dermatan Sulfate; D002809:Chondroitin Sulfates; D003094:Collagen; D010100:Oxygen