title: An autoradiographic study of cell kinetics in epidermis of the toad Bufo bufo bufo (L).
abstract: Under normal conditions, the toad Bufo bufo bufo molts about once a week. It was considered of interest to study whether the rhythmic pattern of cell loss through shedding of a stratum corneum is correlated with a rhythmic production of cells in stratum germinativum. An autoradiographic investigation of cell kinetics in toad skin using [3H]-thymidine and [14C]-thymidine as precursors revealed that cell production in stratum germinativum is a continuous process, and the cell production rate is much higher than is required for replacement of the cells lost with each molt. The parameters measured, i.e., % labeled cells, length of cell cycle, and S-phase, as well as migration of labeled cells from stratum germinativum toward stratum corneum support the assumption of an inhomogeneous cell population in stratum germinativum. A model proposed by Potten form the cellular organization of mouse epithelium provides a useful basis for the interpretation of the results obtained in the present study.
mesh_terms: D000818:Animals; D001345:Autoradiography; D002023:Bufo bufo / Q000033:anatomy & histology*; D002453:Cell Cycle; D002455:Cell Division; D000078404:Epidermal Cells; D007700:Kinetics; D008297:Male; D008433:Mathematics; D012867:Skin / Q000166:cytology*; D013997:Time Factors