Proteins and glycoproteins in plasma membranes and in the membrane lamellae produced by purified oligodendroglia in culture.
Oligodendroglial plasma membranes are complex structures composed of a heterogeneous mixture of proteins and glycoproteins. The Coomassie stained gel patterns showed a maximum of 40 proteins with molecular weights ranging from greater than 200,000 to 12,500. Autoradiography was used to detect binding of radioiodinated lectins to glycoproteins. With concanavalin A, 5 major glycoproteins were seen; with wheat germ agglutinin, 2 major glycoproteins with approximate molecular weights of 95,000 and 78,000 were found; with Ulex europaeus, 7 major glycoproteins were observed. Additional minor bands were also seen. The impermeant probe diazodi[125I]iodosulfanilic acid was used to radiolabel intact cells. It was found that 5 major proteins were radiolabeled in the plasma membranes. In all cases, the whorls of membrane lamellae produced in culture by oligodendroglia tend to have a somewhat less complicated pattern with fewer proteins and glycoproteins than the plasma membranes. However, the whorls of membrane lamellae have far more complicated protein patterns than myelin.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002462:Cell Membrane / Q000032:analysis; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis*; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009457:Neuroglia / Q000032:analysis*; D009836:Oligodendroglia / Q000032:analysis*; D011975:Receptors, Mitogen / Q000032:analysis
D006023:Glycoproteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011975:Receptors, Mitogen