title: Proton nuclear overhauser effect study of the structure of an actinomycin D complex with a self-complementary tetranucleoside triphosphate.
abstract: Saturation transfer and nuclear Overhauser effect (NOE) techniques have been used to assign some resonances of nonexchangeable protons in the NMR spectrum of the complex formed between actinomycin D and the self-complementary tetranucleoside triphosphate d(A-G-C-T). Intermolecular NOEs suggest that the drug chromophore intercalates between the two G-C base pairs of the nucleotide double helix, while the pentapeptide lactone rings fill the minor groove. Binding-induced distortions of helix geometry are discussed.
mesh_terms: D001665:Binding Sites; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003609:Dactinomycin; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008968:Molecular Conformation; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides
chemical_list: D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D003609:Dactinomycin