Platelet glutathione peroxidase activity as an index of selenium status in rats.
Glutathione peroxidase activity in platelets increased stepwise in selenium-depleted rats that were repleted with graded levels of dietary sodium selenite. In a 3-phase depletion/repletion/depletion feeding study, glutathione peroxidase activity was similar in platelets and liver, which apparently contains the largest labile pool of selenium in the body. The activity of glutathione S-transferase (selenium-independent glutathione peroxidase) in platelets was low and was not affected by selenium deficiency, even though hepatic transferase was markedly elevated in selenium-deficient rats. Vitamin E deficiency did not affect activities of glutathione peroxidase or glutathione S-transferase in platelets or liver. Determination of glutathione peroxidase activity in platelets apparently is a promising technique for assessing selenium status and, possibly, for measuring selenium bioavailability.
D000818:Animals; D001682:Biological Availability; D001792:Blood Platelets / Q000201:enzymology*; D004032:Diet; D005979:Glutathione Peroxidase / Q000097:blood* / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008297:Male; D010544:Peroxidases / Q000097:blood*; D051381:Rats; D011916:Rats, Inbred F344; D012643:Selenium / Q000008:administration & dosage / Q000172:deficiency* / Q000378:metabolism
D010544:Peroxidases; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D012643:Selenium