Stimulation of 12-lipoxygenase activity in rat platelets by 17 beta-estradiol.
The effects of estradiol on the endogenous fatty acid (specifically, arachidonic acid) composition of cellular phospholipid fractions and the 12-lipoxygenase activity in rat platelets in vivo were studied. Estradiol had no significant effect on the endogenous fatty acid composition of cellular phospholipid fractions. However, estradiol significantly increased 12-lipoxygenase activity in platelets in a dose-dependent manner. The stimulatory effect of estradiol on platelet lipoxygenase was blocked by the anti-estrogen nafoxidine hydrochloride which was injected simultaneously together with estradiol in vivo, suggesting that the effect in target cells was due directly to estradiol.
D000818:Animals; D001091:Arachidonate Lipoxygenases; D001792:Blood Platelets / Q000201:enzymology*; D002849:Chromatography, Gas / Q000379:methods; D003593:Cytoplasm / Q000201:enzymology; D004958:Estradiol / Q000494:pharmacology*; D005227:Fatty Acids / Q000097:blood; D012739:Gonadal Steroid Hormones / Q000494:pharmacology; D008084:Lipoxygenase / Q000097:blood*; D008297:Male; D009256:Nafoxidine / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011959:Receptors, Estradiol; D011960:Receptors, Estrogen / Q000378:metabolism
D005227:Fatty Acids; D012739:Gonadal Steroid Hormones; D011506:Proteins; D011959:Receptors, Estradiol; D011960:Receptors, Estrogen; D009256:Nafoxidine; D004958:Estradiol; D001091:Arachidonate Lipoxygenases; D008084:Lipoxygenase