The occurrence of gamma-carboxyglutamate in a protein isolated from ox liver mitochondria.
A calcium-binding protein has been isolated from ox liver mitochondria. The purification procedure includes ammonium sulfate fractionation and chromatographies on Sephadex G-100 and DEAE-Sephadex, respectively. The isolated protein contains gamma-carboxyglutamate, has a molecular weight of 59,000, and an isoelectric point of about 3.8. By submitochondrial fractionation it was shown that the calcium-binding protein is located between the inner and outer mitochondrial membranes.
D015055:1-Carboxyglutamic Acid / Q000302:isolation & purification*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000302:isolation & purification*; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D005971:Glutamates / Q000302:isolation & purification*; D007425:Intracellular Membranes / Q000378:metabolism; D007526:Isoelectric Point; D008930:Mitochondria, Liver / Q000378:metabolism*; D008970:Molecular Weight
D002135:Calcium-Binding Proteins; D005971:Glutamates; D015055:1-Carboxyglutamic Acid; D002118:Calcium