Purification and genetic control of NAD-dependent glutamate dehydrogenase from Drosophila melanogaster.
Glutamate dehydrogenase has been purified to near-homogeneity from mature larvae of Drosophila melanogaster. The enzyme has a molecular weight of 347,000 measured by sucrose gradient sedimentation and 343,000 measured by variable-porosity acrylamide gel electrophoresis. Electrophoresis under denaturing conditions showed that the enzyme consists of six subunits of molecular weight 57,000. The structural gene for GDH has been mapped at 81.7 +/- 0.8 on the third chromosome by means of an electrophoretic variant.
D000818:Animals; D002875:Chromosomes / Q000201:enzymology; D003433:Crosses, Genetic; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000235:genetics; D005260:Female; D005796:Genes; D005969:Glutamate Dehydrogenase / Q000235:genetics* / Q000302:isolation & purification; D007814:Larva / Q000201:enzymology; D046911:Macromolecular Substances; D008297:Male; D008970:Molecular Weight; D009243:NAD
D046911:Macromolecular Substances; D009243:NAD; D005969:Glutamate Dehydrogenase