Post-meiotic cell mediation of behavior in progeny of male rats treated with cyclophosphamide.
Transmission of the effects of paternal pre-fertilization exposure to a genotoxic drug being manifested in the F1 progeny is well established using the traditional rodent dominant lethal protocol [7]. More recently, the induction of genotoxic effects in the F1 progeny following chronic paternal exposure prior to fertilization has been described in rats using behavioral testing as an endpoint [1]. In our initial study, male F344 adult rats were treated chronically with 10 mg/kg cyclophosphamide (CP) or saline for 5 weeks (5 days of daily treatment and 2 days of rest). The present study describes the behavior of progeny whose fathers were treated with a single intraperitoneal injection of 10 mg/kg CP and mated at different times post-injection. This design showed that post-meiotic germ cells are the most sensitive to the effects of CP as seen by behavioral testing of the F1 progeny.
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D003520:Cyclophosphamide / Q000494:pharmacology*; D008297:Male; D008540:Meiosis / Q000187:drug effects*; D051381:Rats; D013091:Spermatogenesis / Q000187:drug effects*; D013997:Time Factors
D003520:Cyclophosphamide