Isolation and partial characterization of the most immunologically reactive antigen from Mycobacterium tuberculosis H37Ra culture filtrate.
The culture filtrate of Mycobacterium tuberculosis H37Ra was fractionated by ammonium sulphate precipitation, diethyl aminoethyl (DEAE) cellulose-Sephadex A-50 column chromatography, preparative isoelectric focusing and polyacrylamide gel electrophoresis to isolate the most antigenic tuberculoprotein. One protein, designated P2, was isolated and determined by immunodiffusion to be the most immunologically reactive antigen present in H37Ra culture filtrate. This protein had a molecular weight of about 22 000, a sedimentation constant of 2.2S and was present in Seibert's A, B, and C proteins.
D000942:Antigens, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D008970:Molecular Weight; D009169:Mycobacterium tuberculosis / Q000276:immunology*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins