Total enzymic synthesis of cholesterol from 4,4,14 alpha-trimethyl-5 alpha-cholesta-8,24-dien-3 beta-ol. Solubilization, resolution, and reconstitution of delta 7-sterol 5-desaturase.
D000818:Animals; D002784:Cholesterol / Q000096:biosynthesis*; D002792:Cholestyramine Resin / Q000494:pharmacology; D003579:Cytochrome Reductases / Q000302:isolation & purification / Q000378:metabolism; D042966:Cytochrome-B(5) Reductase; D007700:Kinetics; D007810:Lanosterol / Q000378:metabolism*; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010088:Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D051381:Rats; D011919:Rats, Inbred Strains
C033144:lathosterol delta-5-dehydrogenase; D002792:Cholestyramine Resin; D007810:Lanosterol; D002784:Cholesterol; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; C027532:methylsterol monooxygenase; D044925:Oxidoreductases Acting on CH-CH Group Donors; D003579:Cytochrome Reductases; D042966:Cytochrome-B(5) Reductase; D009251:NADPH-Ferrihemoprotein Reductase