title: Characterization of a human colonic adenocarcinoma cell line, LS123.
abstract: An epithelial cell line, LS123, was established in 1974 from the second in a series of three primary colonic tumors resected from a Caucasian female. The cell line is aneuploid, releases low concentrations of carcinoembryonic antigen (CEA), fails to grow progressively in nude mice, and forms colonies only in enriched semisolid medium developed for tumor stem cells. LS123 cells grow on confluent cell monolayers and in either low serum or serum-free medium. In the chick embryonic skin assay, LS123 cells grew as a well-differentiated abnormal colonic epithelium with little mitotic activity but with some indication of invasion. On floating collagen gels LS123 cells formed a one to three-cell-layer-thick undifferentiated epithelial sheet. The apparent low invasiveness of the cells of this line is supported by the patient's history of three primary colon tumors without systemic metastases during the past 30 yr. Therefore, although LS123 cells possess several properties associated with neoplasia, they have little invasive potential. Thus, LS123 cells may represent an important model for the study of human colon cancer.
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology* / Q000648:ultrastructure; D000328:Adult; D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002455:Cell Division; D002460:Cell Line; D003110:Colonic Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D003470:Culture Media; D005260:Female; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009368:Neoplasm Transplantation
chemical_list: D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; D003470:Culture Media; C030139:colon-specific antigen