Studies on the stability of 3H-dopamine in neostriatal membrane preparations: effects of Ascorbic acid.
The rate of 3H-dopamine decomposition was determined in buffers and in neostriatal membrane preparations that are commonly used in binding experiments. The rate of decomposition of 3H-dopamine was inhibited considerably by the membrane preparation and by ascorbic acid. Under conditions in which the binding of 3H-dopamine is routinely measured in neostriatal membrane preparations, there was almost no 3H-dopamine decomposition. The data would seem to suggest that the routine addition of very high concentrations of antioxidants is unnecessary when 3H-dopamine binding is being determined.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D001667:Binding, Competitive / Q000187:drug effects; D001711:Biotransformation; D002458:Cell Fractionation; D002462:Cell Membrane / Q000378:metabolism; D003342:Corpus Striatum / Q000648:ultrastructure*; D004298:Dopamine / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D014316:Tritium; D001205:Ascorbic Acid; D004298:Dopamine