Enhancement and depression by inosine of the growth inhibitory action of 5-fluorouracil on cultured Jensen tumor cells.
Continuous exposure of Jensen tumor cells in vitro to 1 mM inosine following a 1 hr exposure to 5-fluorouracil resulted in a 5-fold potentiation of growth inhibition. This effect was abolished by the simultaneous presence of 1 mM cytidine and was attributable to altered metabolic processing of drug anabolites after the uptake of 5-fluorouracil had ceased. In contrast, antagonism to 5-fluorouracil was seen when the cells were exposed successively to 1 mM inosine for 1.5 hr and to 5-fluorouracil for 1 hr. In this case the inhibitory action of the drug was diminished by nearly one-half. Quantitation of the potentiation and antagonism was based upon growth delays measured from growth curves obtained by serial photomicrography.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003562:Cytidine / Q000494:pharmacology; D004357:Drug Synergism; D005472:Fluorouracil / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007288:Inosine / Q000494:pharmacology*; D009374:Neoplasms, Experimental / Q000378:metabolism*; D051381:Rats
D007288:Inosine; D003562:Cytidine; D005472:Fluorouracil