Improved measurement of acetylsalicylic acid esterase in serum.
A fixed-time incubation method for measuring acetylsalicylic acid esterase was improved by using a higher concentration of the substrate, acetylsalicylic acid, and an activator, Ca2+. The enzymatic activity in serum was 25-fold that measured by earlier methods. Inhibition studies showed a pattern similar to that reported for cholinesterase. Use of cholinesterase inhibitor in sample tubes for determination of acetylsalicylic acid and salicylic acid is recommended.
D000328:Adult; D002118:Calcium / Q000494:pharmacology; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors / Q000097:blood*; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D008722:Methods; D013696:Temperature
D002265:Carboxylic Ester Hydrolases; C018737:acetylsalicylic acid hydrolase; D002118:Calcium