[Countercurrent distribution in a 2-phase polymer system of cultured cells following their exposure to trypsin, versen, dimethyl sulfoxide and suboptimal temperature].
A study was made of the effects of trypsin (0.25%) EDTA (0.02%), DMSO (10%) and suboptimal temperatures (16-18 degrees C) on the line L cells of monolayer and suspension cultures by the method of counter-current distribution in two-phase polymer system Dextran-500/poly (ethylene) glycol-6000. It has been shown that these agents cause the increase in number of cells with low partition coefficient, which is most expressed in the population of monolayer subline cells.
D000818:Animals; D002478:Cells, Cultured; D003377:Countercurrent Distribution; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology*; D007739:L Cells / Q000187:drug effects*; D051379:Mice; D013696:Temperature; D014357:Trypsin / Q000494:pharmacology*
D004492:Edetic Acid; D014357:Trypsin; D004121:Dimethyl Sulfoxide
Protivotochnoe raspredelenie v dvukhfaznoĭ polimernoĭ sisteme kul'tiviruemykh kletok posle vozdeĭstviia na nikh tripsina, versena, dimetilsul'foksida i suboptimal'noĭ temperatury.