Intracellular transport of cholesterol to the plasma membrane.
We have modified a plasma membrane isolation procedure which utilizes DEAE-Sephadex beads (Gotlib, L. J., and Searls, D. B. (1980) Biochim. Biophys. Acta 602, 207-212) to rapidly measure intracellular transport of cholesterol from the site of synthesis in the endoplasmic reticulum to the plasma membrane. This transport process is rapid, with a half-time of about 10 min, has different kinetics from that of intracellular glycoprotein transport, and appears to be energy-dependent.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002458:Cell Fractionation; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D002784:Cholesterol / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D005700:Galactosyltransferases / Q000378:metabolism; D007700:Kinetics; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010053:Ovary; D013385:Succinate Dehydrogenase / Q000378:metabolism
D002784:Cholesterol; D013385:Succinate Dehydrogenase; D009251:NADPH-Ferrihemoprotein Reductase; D005700:Galactosyltransferases; D000135:Acid Phosphatase