Clinical implications of differences between two recommended procedures for determination of aspartate aminotransferase.
We compared two officially recommended methods for determination of aspartate aminotransferase (EC 2.6.1.1): that of the International Federation of Clinical Chemistry (IFCC) and that of the Deutsche Gesellschaft für Klinische Chemie (DGKC). We used automated enzyme analyzers, initiating the reactions with 2-oxoglutarate. Normal values, 10-30 U/L (IFCC) and 7-18 U/L (DGKC), were apparently insensitive to intra-individual variations. Samples obtained from patients with heart disease showed a markedly different amount of activation with pyridoxal phosphate as compared with samples from other patient categories. Ratios for aspartate aminotransferase/alanine aminotransferase, as used in the differentiation of liver disease, can still be used with either method for determination of aspartate aminotransferase.
D000410:Alanine Transaminase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood*; D002624:Chemistry, Clinical / Q000592:standards*; D005260:Female; D006331:Heart Diseases / Q000097:blood; D006801:Humans; D007674:Kidney Diseases / Q000097:blood; D008107:Liver Diseases / Q000097:blood; D008297:Male; D008722:Methods; D011732:Pyridoxal Phosphate; D013223:Statistics as Topic
D011732:Pyridoxal Phosphate; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase