Parathyroid hormone stimulates adenylate cyclase in rat cerebral microvessels.
We have studied the effect of parathyroid hormone (PTH) on adenylate cyclase of microvessels isolated from rat cerebral cortex. Native bovine (b) PTH-(1-84), the synthetic amino-terminal fragment bPTH-(1-34) and the synthetic analog [Nle8,Nle18,Tyr34]-bPTH- (1-34) amide stimulated adenylate cyclase in a dose-dependent manner with apparent ED50 values of 16 nM, 6.3 nM and 15 nM respectively. The stimulation by bPTH was greatly enhanced by guanosine triphosphate. The PTH antagonist, [Nle8,Nle18,Tyr34]-bPTH-(3-34) amide inhibited the action of bPTH-(1-84) and bPTH-(1-34). In summary, PTH stimulated adenylate cyclase in rat cerebral microvessels in a very similar manner to its stimulation in the renal cortex.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008833:Microcirculation; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D010281:Parathyroid Hormone; D010446:Peptide Fragments; C025076:parathyroid hormone (1-34), bovine; C025497:parathyroid hormone (3-34) amide, Nle(8,18)-Nle(34)-; D011433:Propranolol; D000262:Adenylyl Cyclases; D007545:Isoproterenol; D010646:Phentolamine