Tetanus toxin inhibition of K+-stimulated [3H]GABA release from developing cell cultures of the rat cerebellum.
The effect of tetanus toxin pretreatment on K+-stimulated [3H]gamma-aminobutyric acid release from neuron-enriched cerebellar cell cultures at various stages during their development in vitro was assessed. Tetanus toxin had little inhibitory effect on immature (1-3-day-old) cultures, but markedly reduced K+-evoked [3H]gamma-aminobutyric acid release from 7- and 14-day-old cultures (approximately 80% inhibition). It is suggested that cerebellar neurons in culture develop tetanus toxin-sensitive transmitter release mechanisms similar to their in vivo counterparts.
D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology*; D051381:Rats; D013744:Tetanus Toxin / Q000494:pharmacology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D013744:Tetanus Toxin; D005680:gamma-Aminobutyric Acid; D011188:Potassium