Immunoblot detection of pyridoxal phosphate binding proteins in liver and hepatoma cytosolic extracts.
A monoclonal antibody, highly selective for the 5'-phosphopyridoxyl group, can be used to detect cytosolic pyridoxal-5'-phosphate binding proteins by an immunoblot procedure. This technique, when applied to sodium borohydride-treated cytosolic extracts obtained from normal rat liver at various stages of development as well as several liver-derived Morris hepatomas, reveals patterns of pyridoxal-5'-phosphate binding proteins that are characteristic of the various sources of cytosol. These findings suggest that there are developmental and tumor-specific requirements for pyridoxal-5'-phosphate, the coenzymatically active form of vitamin B-6.
D000375:Aging; D000818:Animals; D000911:Antibodies, Monoclonal; D002352:Carrier Proteins / Q000302:isolation & purification*; D003600:Cytosol / Q000378:metabolism*; D007120:Immunochemistry; D008099:Liver / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008297:Male; D009363:Neoplasm Proteins / Q000302:isolation & purification; D011732:Pyridoxal Phosphate / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D009363:Neoplasm Proteins; D011732:Pyridoxal Phosphate