The effect of the scorpion venom, tityustoxin, on high-affinity choline uptake in rat brain cortical slices.
Tityustoxin (TsTx) inhibited high affinity choline uptake (HAChU) in cortical slices of the rat brain. The effect was dependent on the concentration of tityustoxin, energy source, incubation time, temperature, and the pH of the incubation medium. The inhibitory effect was dependent upon the presence of sodium and calcium ions in the incubation medium; barium ions could not replace calcium. Both tetrodotoxin and ethyleneglycol-tetra-acetic acid (EGTA) blocked the inhibitory effect of tityustoxin on high affinity choline uptake. On this evidence, it is suggested that the effect of tityustoxin might be related to its action on cell depolarization, causing an increase in the release of acetylcholine (ACh).
D000818:Animals; D002412:Cations / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D002794:Choline / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009498:Neurotoxins / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D012604:Scorpion Venoms / Q000494:pharmacology*; D012964:Sodium / Q000494:pharmacology; D013696:Temperature; D013779:Tetrodotoxin / Q000494:pharmacology
D002412:Cations; D009498:Neurotoxins; D012604:Scorpion Venoms; C003609:tityustoxin; D013779:Tetrodotoxin; D012964:Sodium; D005947:Glucose; D002794:Choline; D011188:Potassium