Chemical modification of cytosine residues of mouse 5 S ribosomal RNA with hydrogen sulfide. (Nucleosides and nucleotides 43).
Cytosine residues of nucleic acids were converted to 4-thiouracil residues with hydrogen sulfide in pyridine and water to examine the secondary and tertiary structures of mouse 5 S rRNA. The cytosine residues at positions 10, 24, 34 (or 36), 39, 44 (or 46) and 63 were converted preferentially when the treatment was carried out at 28 degrees C. This result supports the model of the secondary structure of 5 S rRNA of Nishikawa, K. and Takemura, S. ((1974) FEBS Lett. 40, 106-109) consisting of five helices and five loops. As the temperature was increased to 35 degrees C, additional cytosine residues in positions 26, 52 and 78 were modified to moderate extents.
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003596:Cytosine; D004591:Electrophoresis, Polyacrylamide Gel; D006862:Hydrogen Sulfide; D007668:Kidney; D051379:Mice; D008809:Mice, Inbred C3H; D012335:RNA, Ribosomal; D012260:Ribonucleases; D013889:Thiouracil
D012335:RNA, Ribosomal; D013889:Thiouracil; D003596:Cytosine; D012260:Ribonucleases; D006862:Hydrogen Sulfide