Toxic activity of seventeen industrial solvents and halogenated compounds on human lymphocytes cultured in vitro.
Seventeen chemicals (solvents, insecticides and intermediates in the production of textiles and resins) were tested in a short-term in vitro system with human lymphocytes to determine their toxic action. The parameters studied were the tritiated thymidine uptake and cell viability in cultures grown with or without a rat liver metabolizing system (S-9 mix). Data obtained showed that 1,3-dichlorobenzene, 1,2-dichlorobenzene, hexane, 1,2-diiodoethane, 1,4-dichlorobenzene, tetrachloroethylene, 2,3-dibromopropanol, chloromethyl methyl ether, 1,2- and 1,3-dibromopropane, in order, exerted the more toxic effects; ethyl acetate, cyclohexane, cyclohexanone and benzene showed lower toxic activity. The chemicals lost their toxic power in the presence of the metabolizing system with the exception of 1,2- and 1,3-dichlorobenzene which maintained in some degree their toxicity even in the presence of the S-9 mix. Only chloromethyl methyl ether elicited unscheduled DNA synthesis acting as DNA damaging agent.
D000328:Adult; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D006846:Hydrocarbons, Halogenated / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D012997:Solvents / Q000633:toxicity*; D013936:Thymidine / Q000378:metabolism
D006846:Hydrocarbons, Halogenated; D012997:Solvents; D004247:DNA; D013936:Thymidine