Flow cytometric analysis of lectin binding to human peripheral blood lymphocytes.
The binding of fluorescein-conjugated lentil lectin and concanavalin A to the surface membrane of human peripheral blood lymphocytes was studied by flow cytometry. The lymphocytes bound 3-fold more lentil lectin molecules compared with concanavalin A molecules and lentil lectin binding approached saturation at a much lower concentration than did that of concanavalin A. Lentil lectin identified two groups of lymphocytes: a low-binding T-cell fraction and a high-binding B-cell-enriched fraction. Concanavalin A did not discriminate between these populations in unseparated lymphocytes. Competition studies indicated that lentil lectin and concanavalin A were bound to different sites on the lymphocyte surface, although about 50% of lentil lectin sites were in close proximity to concanavalin A sites.
D000328:Adult; D002462:Cell Membrane / Q000276:immunology; D002469:Cell Separation; D003208:Concanavalin A / Q000276:immunology; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D011975:Receptors, Mitogen / Q000276:immunology; D012397:Rosette Formation; D013861:Thiocyanates
D005452:Fluoresceins; D005456:Fluorescent Dyes; D037102:Lectins; D011975:Receptors, Mitogen; D013861:Thiocyanates; D003208:Concanavalin A; D016650:Fluorescein-5-isothiocyanate