Characterization of human colon carcinoma cell lines isolated from a single primary tumour.
The initiation of a cultured human colon carcinoma line on a feeder layer of confluent fibroblasts is described. Attempts to initiate cultures without fibroblast feeder layers were not successful. Two sub-lines (designated HCT C and HCT C Col) were isolated and weaned from cells growing on the surface of the feeder layer. The sub-lines had different morphologies, secreted different levels of carcinoembryonic antigen (CEA) into the medium of confluent cultures and had slightly different karyotypes. Both sub-lines grew in semi-solid medium and formed xenografts when injected s.c. into athymic nude mice. Analysis of radioiodinated cell membrane components indicated small, but significant differences between the sub-lines.
D000818:Animals; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis; D003110:Colonic Neoplasms / Q000032:analysis / Q000235:genetics / Q000473:pathology*; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D007621:Karyotyping; D008677:Metaphase; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous
D002272:Carcinoembryonic Antigen