[A method for the ultrastructural, immunocytochemical detection of ecdysone].
The investigations were carried out with prothoracic glands of Galleria mellonella. The influence of various fixation media on the preservation of the ultrastructure and on the immunocytochemical staining of the steroid ecdysone was studied. An immunocytochemical staining technique for ecdysone is described allowing the ultrastructural localization of ecdysone in different cellular compartments.
D000818:Animals; D001181:Arthropods / Q000032:analysis* / Q000648:ultrastructure; D004285:Dogs; D004440:Ecdysone / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry / Q000379:methods*; D008854:Microscopy, Electron; D013347:Subcellular Fractions / Q000032:analysis / Q000648:ultrastructure
D004440:Ecdysone
Untersuchungen zur Methodik des ultrastrukturellen immuncytochemischen Nachweises von Ecdysteroiden.