[Demonstration of Newcastle disease virus by enzyme-linked immunosorbent assay].
Investigations were carried out with regard to the application of the sensitive, rapid, and effective enzyme-linked immunosorbent assay (ELISA) in the demonstration of the Newcastle disease virus (NDV). Used was the peroxydase-marked immunoglobulin fraction of the anti-DV rabbit serum in the microconditions of the double-antibody method. The demonstration of the NDV was in a cultivation liquid of NDV-infected BHK21 cells. As control served intact cells as well as strains Roussev and La Sota of NDV pruified through ultracentrifugation on an isokinetic saccharose gradiend. It is stated that ELISA is much more sensitive than the hemagglutination test and the agar gel immunodiffusion test.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002478:Cells, Cultured; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests; D005779:Immunodiffusion; D009522:Newcastle disease virus / Q000276:immunology*; D011817:Rabbits
D000956:Antigens, Viral
Dokazvane virusa na Niukiasulskata bolest (NDV) chrez enzim-svurzaniia imunosorbenten metod.