A simple and rapid method for isolation of retinal S antigen.
A procedure is described for the rapid isolation of S antigen from retinal tissue, by salt precipitation, gel filtration and final purification by adsorption chromatography on hydroxyapatite-agarose. The method yielded a highly purified material and allowed an excellent preservation of the antigenicity, as shown by high pathogenic activity in induction of experimental autoimmune uveoretinitis.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification*; D019393:Arrestin; D001324:Autoantigens / Q000302:isolation & purification*; D002417:Cattle; D002825:Chorioretinitis / Q000276:immunology*; D002845:Chromatography; D002850:Chromatography, Gel; D005779:Immunodiffusion
D000941:Antigens; D019393:Arrestin; D001324:Autoantigens