Enhancement of the 7 alpha-dehydroxylase activity of a gram-positive intestinal anaerobe by flavins.
The addition of flavins to the growth medium specifically enhanced the 7alpha-dehydroxylation of bile acids by anaerobically growing cultures of a Eubacterium lentum-like intestinal anaerobe, strain c-25, without an increase in cell growth. The order of the enhancement of the reaction was flavin adenine dinucleotide > flavin mononucleotide >> riboflavin.
D005051:Eubacterium / Q000201:enzymology*; D005486:Flavin Mononucleotide / Q000494:pharmacology; D005182:Flavin-Adenine Dinucleotide / Q000494:pharmacology; D005415:Flavins / Q000494:pharmacology*; D006913:Hydroxysteroid Dehydrogenases; D007422:Intestines / Q000382:microbiology; D010088:Oxidoreductases; D012256:Riboflavin / Q000494:pharmacology; D013250:Steroid Hydroxylases / Q000378:metabolism*
D005415:Flavins; D005182:Flavin-Adenine Dinucleotide; D005486:Flavin Mononucleotide; D010088:Oxidoreductases; D006913:Hydroxysteroid Dehydrogenases; C025376:7 alpha-hydroxysteroid dehydrogenase; D013250:Steroid Hydroxylases; D012256:Riboflavin