Inhibition of DNA polymerases of sea urchin by palmitoyl coenzyme A.
Palmitoyl CoA noncompetitively inhibited the activities of DNA polymerase alpha and gamma, prepared from sea urchin germ cells, with Ki values of 28 microM and 116 microM, respectively. Myristoyl CoA also inhibited DNA polymerase alpha and gamma, while coenzyme A, short chain fatty acyl CoA's, Na-myristate and Na-palmitate failed to inhibit the enzymes. It was concluded that both the long hydrocarbon chain and CoA moiety of long chain fatty acyl CoA's are necessary for inhibition of DNA polymerase activity. DNA polymerase beta was not inhibited by long chain fatty acyl CoA's.
D000214:Acyl Coenzyme A / Q000494:pharmacology*; D000818:Animals; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors*; D004258:DNA Polymerase III / Q000037:antagonists & inhibitors*; D007700:Kinetics; D019384:Nucleic Acid Synthesis Inhibitors; D010171:Palmitoyl Coenzyme A / Q000494:pharmacology*; D012617:Sea Urchins / Q000201:enzymology*; D013329:Structure-Activity Relationship
D000214:Acyl Coenzyme A; D019384:Nucleic Acid Synthesis Inhibitors; D010171:Palmitoyl Coenzyme A; D004257:DNA Polymerase II; D004258:DNA Polymerase III