[Automated screening and identification of irregular anti-erythrocyte antibodies].
Screening of irregular antibodies performed on a Groupamatic MG50, a two or three channels auto-analyser, and with manual techniques, revealed 4,445 positive sera which were than identified on this auto-analyser system. It was concluded that the "saline-PVP-4 degrees C" channel recovers 17% of the screened sera, which appeared negative with the bromelin and polybrene channels. Saline -4 degrees C detected sera include all of the -M and -P1 specificities and about half of the -K, -Lea and Leb. Identification of cold and namely anti-I antibodies on this channel also leads to an improved analysis of polyspecific sera. The high sensitivity of this 3 channels auto-analyser seems to be optimal for screening and identification of irregular antibodies.
D000918:Antibody Specificity; D001322:Autoanalysis / Q000295:instrumentation; D001789:Blood Group Antigens / Q000276:immunology*; D001963:Bromelains / Q000494:pharmacology; D006583:Hexadimethrine Bromide / Q000494:pharmacology; D006801:Humans; D007518:Isoantibodies / Q000032:analysis*; D012204:Rh-Hr Blood-Group System / Q000276:immunology
D001789:Blood Group Antigens; D007518:Isoantibodies; D012204:Rh-Hr Blood-Group System; D006583:Hexadimethrine Bromide; D001963:Bromelains
Dépistage et identification automatisés des anticorps irréguliers anti-érythrocytaires.