Mapping of the genes of some components of the electron transport chain (complex I) on the X chromosome of mammals.
This paper describes genetic mapping studies with several respiration-deficient mutants of Chinese hamster fibroblasts which have a defect in complex I of the electron transport chain (NADH-coenzyme Q reductase). The mutations associated with two different complementation groups map on the X chromosome. In two cases (G14 and G20) karyotypic and isozyme analyses in hybrids have shown that a gene(s) on the mouse X chromosome complements the mutation(s) in the hamster cell mutant(s). A cosegregation analysis in hybrid cells has shown the corresponding genes to be linked to the HPRT genes (hamster-mouse hybrids of G14, and hamster-hamster hybrids for G14 and G20). By the same method the defective gene in a third mutant (G4) was also shown to be X-linked. A mutation representing a third complementation group (G11) was shown to be on an autosomal gene. These results provide an explanation for our observation that cells with recessive mutations in complementation groups I and II can be selected at relatively high frequencies.
D000818:Animals; D002478:Cells, Cultured; D002874:Chromosome Mapping; D006224:Cricetinae; D003412:Cricetulus; D004579:Electron Transport; D005260:Female; D005816:Genetic Complementation Test; D007527:Isoenzymes / Q000235:genetics; D007621:Karyotyping; D051379:Mice; D016660:NAD(P)H Dehydrogenase (Quinone); D009247:NADH, NADPH Oxidoreductases / Q000235:genetics*; D011808:Quinone Reductases / Q000235:genetics*; D012730:Sex Chromosomes; D014960:X Chromosome
D007527:Isoenzymes; D009247:NADH, NADPH Oxidoreductases; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases