A solid-phase immunofluorescence assay (SIFA) using membrane filters.
A solid-phase immunofluorescence assay (SIFA) is presented which allows the demonstration of antibodies against soluble antigens. The test is performed by successively incubating nitrate cellulose discs in microtitration plates with anti-Ig, the sample to be tested and FITC-labeled antigen. The fluorescence intensity of the discs is then measured by using a microfluorometer. The assay is simple, needs a small amount of material and allows to test a large number of samples.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000276:immunology; D002417:Cattle; D005455:Fluorescent Antibody Technique / Q000295:instrumentation*; D006041:Goats; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D012709:Serum Albumin / Q000276:immunology; D012756:Sheep
D000906:Antibodies; D000941:Antigens; D012709:Serum Albumin