[Immunocytochemical demonstration of neuropeptides (neurophysin and oxytocin) by the protein A-gold technic].
In the classic peptidergic neurosecretory system of vertebrates the neurohormone oxytocin is associated with the carrier protein neurophysin. By using small gold particles (3 to 12 nm) in the protein A-gold technique, both the hormone and the carrier-protein will be labelled, while larger particles (20 nm) label only the neurophysin. To bind the latter particles, a greater number of Fc-regions of antigen-IgG-complexes seems to be necessary.
D000818:Animals; D006046:Gold; D007032:Hypothalamus, Anterior / Q000032:analysis*; D007106:Immune Sera; D007141:Immunoglobulin Fc Fragments; D007158:Immunologic Techniques; D009481:Neurophysins / Q000032:analysis*; D010121:Oxytocin / Q000032:analysis*; D010286:Paraventricular Hypothalamic Nucleus / Q000032:analysis; D010904:Pituitary Gland, Posterior / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013205:Staphylococcal Protein A; D013495:Supraoptic Nucleus / Q000032:analysis
D007106:Immune Sera; D007141:Immunoglobulin Fc Fragments; D009481:Neurophysins; D013205:Staphylococcal Protein A; D010121:Oxytocin; D006046:Gold
Immuncytochemische Darstellung von Neuropeptiden (Neurophysin und Oxytocin) mit der Protein-A-Gold-Technik.