Properties of an aldose reductase from pig lens. Comparative studies of an aldehyde reductase from pig lens.
Two monomeric NADPH enzymes from pig lens, an aldehyde reductase and an aldose reductase, have been characterized. The aldose reductase is obtained in a pure form. The aldehyde reductase, usually called hexonate dehydrogenase, is the same protein as that was recently isolated from pig liver [Branlant, G. and Biellmann, J.F. (1980) Eur. J. Biochem. 105, 611-621]. The aldose reductase is shown to have a number of properties in common with the aldehyde reductase, namely its physico-chemical properties, its tendency to be inhibited by quercitine derivatives and its substrate specificity. These two enzymes differ in their immunological properties. Only aldose reductase has a reactive Cys residue, localized in or near the substrate binding site. In contrast to that shown for aldehyde reductase [Branlant, G. et al. (1981) Eur. J. Biochem. 116, 505-512; Branlant, G. (1982) Eur. J. Biochem. 121, 407-411], no anion-recognition sites are in the substrate binding site of aldose reductase. The fact that also sugars are substrates for aldose reductase support the idea that this enzyme is implicated in the formation of sugar cataract as suggested by Kinoshita, J.H. et al. [J. Am. Med. Ass. 246, 257-261 (1981)]. Pig lens aldose reductase does not show homotropic cooperative effects with respect to either substrate or coenzyme.
D000429:Alcohol Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D000449:Aldehyde Reductase / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002627:Chemistry, Physical; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D007908:Lens, Crystalline / Q000201:enzymology*; D009249:NADP / Q000378:metabolism; D013379:Substrate Specificity; D013401:Sugar Alcohol Dehydrogenases / Q000302:isolation & purification*; D013552:Swine; D015032:Zinc / Q000494:pharmacology
D009249:NADP; D004228:Dithionitrobenzoic Acid; D000429:Alcohol Oxidoreductases; D013401:Sugar Alcohol Dehydrogenases; C019642:alcohol dehydrogenase (NADP+); D000449:Aldehyde Reductase; D015032:Zinc