Intracellular pH in intact rat renal cortex estimated with [14C]-5,5-dimethyl-2,4-oxazolidinedione.
The distribution of [14C]-5,5-dimethyl-2,4-oxazolidinedione ([14C]-DMO) was measured in intact rat renal cortex using 22Na+ to estimate extracellular fluid volume. Results of two different methods to obtain tissue were compared, a cortical slice technique and a whole kidney frozen technique. In normal animals with a mean blood pH of 7.41 +/- 0.006 (SEM) the cortical slice method measured an intracellular pH of 6.97 +/- 0.03 (SEM). Corrections are described which can be used to compensate for varying volumes of tubular fluid in the slice and for differing concentrations of intracellular sodium. Using reasonable estimates for tubular fluid volume and intracellular sodium these corrections increase the value of intracellular pH to about 7.20. The results of the study indicate that the cortical slice [14C]-DMO method provides a satisfactory technique to obtain baseline values for intracellular pH in intact renal cortex, which can be used to detect changes in intracellular pH produced by experimental manipulations.
D000136:Acid-Base Equilibrium; D000818:Animals; D001826:Body Fluids / Q000502:physiology*; D004114:Dimethadione / Q000652:urine; D005110:Extracellular Space / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid / Q000032:analysis / Q000502:physiology*; D007672:Kidney Cortex / Q000502:physiology*; D008297:Male; D008722:Methods; D010080:Oxazoles; D051381:Rats / Q000502:physiology*; D011919:Rats, Inbred Strains; D012964:Sodium / Q000032:analysis
D010080:Oxazoles; D012964:Sodium; D004114:Dimethadione