title: Changes in the heterogeneity of ribulosebisphosphate carboxylase-oxygenase in winter rye induced by cold hardening.
abstract: The quaternary structures of ribulose-1,5-bisphosphate carboxylase-oxygenase from cold-hardened and unhardened Puma rye were examined by two-dimensional gel electrophoresis according to the method of O'Farrell. The results indicate that major changes in charge heterogeneity occur in the large subunit of this enzyme during growth at cold-hardening temperatures. The extent of charge heterogeneity decreased upon adaptation of Puma rye to cold-hardening temperatures. In addition to charge heterogeneity, molecular weight heterogeneity was also evident In the large subunit polypeptides of the enzyme from cold-hardened and unhardened Puma rye.
mesh_terms: D002262:Carboxy-Lyases / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D003080:Cold Temperature; D002523:Edible Grain / Q000201:enzymology*; D004586:Electrophoresis / Q000379:methods; D005779:Immunodiffusion; D010105:Oxygenases / Q000378:metabolism*; D010455:Peptides / Q000302:isolation & purification; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism*; D012621:Seasons; D012434:Secale / Q000201:enzymology*
chemical_list: D010455:Peptides; D010105:Oxygenases; D002262:Carboxy-Lyases; D012273:Ribulose-Bisphosphate Carboxylase