Calcium and ionophore A23187 induce the sickle cell membrane phosphorylation pattern in normal erythrocytes.
Pre-treatment of normal erythrocytes with micromolar Ca2+ and ionophore A23187 induces abnormal phosphorylation of membrane polypeptides, as determined by labeling with exogenous 32Pi. The Ca2+ -induced effects, which include increased incorporation of 32P into acid-stable linkages and increased labeling in the Band 3 and 4.5-4.9 regions of SDS gels, are similar to those seen in untreated sickle erythrocytes. Part of the abnormal phosphorylation of sickle cells may be caused by their elevated intracellular Ca2+ levels.
D000255:Adenosine Triphosphate / Q000097:blood; D000755:Anemia, Sickle Cell / Q000097:blood*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000502:physiology*; D004912:Erythrocytes / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins / Q000097:blood; D010766:Phosphorylation
D000900:Anti-Bacterial Agents; D008565:Membrane Proteins; D000001:Calcimycin; D000255:Adenosine Triphosphate; D002118:Calcium