[Pleiotropic phenotype characteristics of cells resistant to the plant lectin concanavalin A].
The single-step selection technique involving resistance to 300 micrograms/ml of Concanavalin A (Con-A) aided to select those resistant variants from the clones CHO-K1 line 773 as well as clones CR1EBAG and CR2EBAG which had resistance to 1 microgram/ml of ethidium bromide (EB) and 30 micrograms/ml of 8-asaguanine. The resistance to lectin was found to alter the properties of the transformed phenotype as follows: morphology, saturation density, adhesiveness, ability of cloning in soft--agar, agglutinability. Cell hybrids of intraspecies origin arising from confluence of lectin-sensitive and lectin-resistant lines revealed that the resistance to lectin is inherited by the recessive type. Their growth characteristics, their ability of cloning in soft--agar are similar to those in control hybrid cultures obtained from confluence of sensitive parental cells. The lines selected by their resistance to Con-A with the background resistance to EB maintain both kinds of resistance and inherit them independently: to EB by the dominant type and to Con-A by the recessive type.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells / Q000187:drug effects; D003208:Concanavalin A / Q000037:antagonists & inhibitors*; D006224:Cricetinae; D003412:Cricetulus; D004351:Drug Resistance; D006822:Hybrid Cells / Q000187:drug effects; D010641:Phenotype; D012641:Selection, Genetic
D003208:Concanavalin A
Osobennosti pleĭotropnogo fenotipa kletok, ustoĭchivykh k rastitel'nomu lektinu konkanavalinu A.