Intestinal absorption and urinary excretion of antitumor peptidomannan KS-2 after oral administration in rats.
A peptidomannan fraction designated as KS-2 was subjected for distribution study after oral administration. KS-2, exhibiting antitumor- and interferon-inducing action, was isolated from hot water extracts of mycelia of Basidiomycetes. For this purpose, KS-2 was labelled with fluorescein isothiocyanate. The amount and location of the labelled KS-2 (F-KS) was determined quantitatively by fluorescence intensity, and the molecular sizes were estimated by the fluorescence polarization method. The present data indicated that F-KS, when given orally, was absorbed partially via portal vein and intestinal lymphatics into the general circulation with intact molecular size. Moreover, F-KS was recovered in urine with concomitant breakdown in molecular weight. Histological evaluation revealed that F-KS was accumulated in the mesenteric lymph nodes, Peyer's patches, spleen, liver, and kidneys. These findings provide evidence that the polysaccharide KS-2 becomes accessible to the immune tissues and the vascular system; thus it may exert potentiating activity of defense system.
D000284:Administration, Oral; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D019793:Fluorescein; D005452:Fluoresceins; D007408:Intestinal Absorption; D008208:Lymphatic System / Q000378:metabolism; D008297:Male; D011509:Proteoglycans / Q000652:urine*; D051381:Rats
D001798:Blood Proteins; D005452:Fluoresceins; D011509:Proteoglycans; C010770:polysaccharide-K; D019793:Fluorescein