Xanthone-2-carboxylic acid effect on lens growth, hydration and proteins during diabetic cataract development.
The concomitant protective effects of the aldose reductase inhibitor, 7-dimethylsulfamoyl-xanthone-2-carboxylic acid, were established by three lens parameters (soluble crystallin proteins, growth and cell hydration) because their quantitation provided a comprehensive index of lens physiology during sugar cataractogenesis in the rat neonate. Their fused eyelids provided the orbital pouch for topical administration of inhibitor to the treated lens; the contralateral pouch served as an untreated control. Protein preservation was determined by gel filtration chromatography. In galactose-maintained neonates, untreated lenses exhibited only 50% of the normal Fraction II protein whereas xanthone-treatment maintained 73% of this component. Quantitative analysis of scanning electron micrographs indicated that xanthone-treatment partially protected lenses against both intra and extracellular fluid accumulation as determined by measurements of individual fiber cell thickness, density (the number of cells/10 micron cortex), and interdigitation. In addition, xanthone-treatment improved lens growth as evidenced by radius and dry weight measurements. Our results suggest that topically applied xanthone partially inhibited sugar cataractogenesis.
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D000818:Animals; D000831:Animals, Newborn; D001834:Body Water / Q000378:metabolism; D002386:Cataract / Q000209:etiology / Q000517:prevention & control*; D003459:Crystallins / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D005690:Galactose / Q000494:pharmacology; D007908:Lens, Crystalline / Q000187:drug effects* / Q000254:growth & development; D051381:Rats; D013401:Sugar Alcohol Dehydrogenases / Q000037:antagonists & inhibitors*; D014966:Xanthenes / Q000494:pharmacology*; D044004:Xanthones
D003459:Crystallins; D014966:Xanthenes; D044004:Xanthones; C036484:7-dimethylsulfamoylxanthone-2-carboxylic acid; D013401:Sugar Alcohol Dehydrogenases; D000449:Aldehyde Reductase; D005690:Galactose