One-step screening method for the polymorphism of apolipoproteins A-I, A-II, and A-IV.
Apolipoprotein A-I exhibits a polymorphism that can be easily investigated in native serum by a simple method involving incubation of serum in the presence of decylsulfate and beta-mercaptoethanol and subsequent isoelectric focusing. From six to eight proteins can be separated in a pH gradient from 4 to 6 and thus patients with apolipoprotein A-I variants can be distinguished from normal persons. This method also permits monitoring for polymorphic forms of apoA-II and apoA-IV as well as detection of C apolipoproteins. To verify the identity of the different apolipoproteins, a two-dimensional electrophoresis technique was applied, with an SDS system for the second dimension. In addition, monospecific antibodies for apolipoproteins A-I, A-II, and A-IV were used for the immunological identification. The method described here led to the discovery of three different familial apolipoprotein A-I variants.
D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D001053:Apolipoproteins / Q000235:genetics*; D001054:Apolipoproteins A; D005260:Female; D005838:Genotype; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D008722:Methods; D010375:Pedigree; D011110:Polymorphism, Genetic
D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D001053:Apolipoproteins; D001054:Apolipoproteins A; C022205:apolipoprotein A-IV