Insolubilization of hydroxyproline-rich cell wall glycoprotein in aerated carrot root slices.
The hydroxyproline-rich glycoprotein of plant cell walls is secreted from the cytoplasm as a soluble monomer which slowly becomes insolubilized. A tyrosine derivative, isodityrosine, is formed in the cell wall during this insolubilization and could serve as a protein-protein crosslink. Glycoprotein insolubilization is inhibited by peroxidase inhibitors and free radical scavengers, the most effective of which is L-ascorbate. These data support a hypothesis that the hydroxyproline-rich cell wall glycoprotein forms a covalently crosslinked wall network under the control of an extracellular peroxidase/ascorbate oxidase system.
D001704:Biopolymers; D002473:Cell Wall / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D006023:Glycoproteins / Q000302:isolation & purification*; D006909:Hydroxyproline / Q000302:isolation & purification*; D010944:Plants / Q000032:analysis*; D012995:Solubility
D001704:Biopolymers; D006023:Glycoproteins; D006909:Hydroxyproline