Quantitative bacteriological analysis of amniotic fluid.
Quantitative bacteriological analysis of amniotic fluid (AF) was performed on 60 fluid samples collected by catheter from 50 selected labor patients regarded as liable to infection. AF cultures were positive in 52 cases. The bacterial colony counts ranged from less than 10(2) colony-forming units (CFU) per milliliter to 10(9) CFU/ml. Pathogenic bacteria in neonates such as group B Streptococcus, Escherichia coli, and Listeria monocytogenes were encountered with numerations equal to or greater than 10(7) CFU/ml in groups with clinical findings. An excellent correlation was noted between bacterial counts in AF and clinical data (p less than 0.00006). The results confirm that the quantitative bacteria analysis of the AF is a useful tool for evaluation of an infection risk for neonates.
D000653:Amniotic Fluid / Q000382:microbiology*; D001424:Bacterial Infections / Q000175:diagnosis* / Q000209:etiology; D013293:Enterococcus faecalis / Q000302:isolation & purification; D004926:Escherichia coli / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000175:diagnosis* / Q000209:etiology; D007958:Leukocyte Count; D008089:Listeria monocytogenes / Q000302:isolation & purification; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D013210:Staphylococcus / Q000302:isolation & purification; D013292:Streptococcus agalactiae / Q000302:isolation & purification