The influence of L-4-oxalysine on carbon tetrachloride-induced changes in drug-metabolizing enzyme activity of mouse liver.
1. Carbon tetrachloride (0.2 ml/kg) and L-4-oxalysine (200 mg/kg per day for two days) were administered to male mice separately, and together, and the liver drug-metabolizing parameters measured. 2. Carbon tetrachloride alone depleted hepatic microsomal protein content by 50% and cytosolic sulphydryl compounds by 90%; oxalysine alone had no effect. 3. Microsomal cytochrome P-450 was decreased by 60%, cytochrome b5 by 30%, ethylmorphine N-demethylation by 50% and 7-ethyoxycoumarin O-deethylation by 80% following carbon tetrachloride administration; oxalysine had no effect on these losses. 4. After administration of carbon tetrachloride, cytosolic glutathione S-transferase activity was decreased by 16%; this effect was not seen when oxalysine and carbon tetrachloride were given together. Oxalysine alone slightly increased this enzyme activity.
D000600:Amino Acids, Dicarboxylic; D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051379:Mice; D012694:Serine / Q000031:analogs & derivatives* / Q000494:pharmacology
D000600:Amino Acids, Dicarboxylic; D012694:Serine; D003577:Cytochrome P-450 Enzyme System; C027136:4-oxalysine; D005982:Glutathione Transferase