Calcium detection in secretion granules of avian oviduct by scanning electron microscopy (SEM) and energy-dispersive X-ray microanalysis (EDX).
Secretion granules in the shell gland, isthmus, and albumin-secreting region of the hen oviduct were analyzed with WET-scanning electron microscopy (SEM) and EDX, a combination of wide-angle backscattered electron detector (BED) and energy-dispersive X-ray microanalyzer (EDX). Glutaraldehyde-fixed but unhydrated, unstained, and uncoated samples were analyzed; Ca was localized in all secretion granules in all three sections of the hen oviduct studied.
D000818:Animals; D002118:Calcium / Q000032:analysis*; D002645:Chickens; D003594:Cytoplasmic Granules / Q000032:analysis*; D004577:Electron Probe Microanalysis; D005260:Female; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D010057:Oviducts / Q000032:analysis* / Q000648:ultrastructure
D002118:Calcium