title: Prostaglandins and thromboxane: tert.-butyldimethylsilyl ethers as derivatives for their analyses by gas chromatography-mass spectrometry-selected ion monitoring.
abstract: tert.-Butyldimethylsilyl ethers of eighteen prostaglandins (PGs) and thromboxane have been analyzed by combined packed-column or fused-silica capillary gas chromatography-mass spectrometry (GC-MS). Except for thromboxane B2 and PGs of the 3 series the [M - 57] ion, the loss from the molecular ion of the tert.-butyl radical, is generally the major peak in the spectra of PGs. This high abundance of large fragments provides suitable criteria for the employment of these derivatives in GC-MS selected ion monitoring for rapid quantity estimation of PGs from biological samples. PGs from rat stomach fundus were rapidly quantitated in a single chromatogram using this procedure.
mesh_terms: D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D005748:Gastric Fundus / Q000032:analysis; D017646:Organosilicon Compounds; D011453:Prostaglandins / Q000032:analysis*; D051381:Rats; D012825:Silicon / Q000032:analysis*; D013931:Thromboxanes / Q000032:analysis*
chemical_list: D017646:Organosilicon Compounds; D011453:Prostaglandins; D013931:Thromboxanes; C009660:t-butyldimethylsilyl compounds; D012825:Silicon