Chemical and immunological identification of glycolipid-based blood group ABH and Lewis antigens in human kidney.
A polar non-acid glycolipid fraction has been isolated from human kidney. It was shown by thin-layer chromatography to be a mixture of glycolipids having more than four carbohydrate residues. Immunological testing revealed strong blood group Lea and A activity together with weak Leb, P1 and B activity. Mass spectrometry of the permethylated and permethylated-reduced (LiAlH4) glycolipid fraction showed that the two major components were a five sugar fucolipid (isomer of Lea) and a glycolipid having four hexoses and one N-acetylhexosamine. In addition, blood group Leb, B and A type hexaglycosylceramides were present. Evidence for small amounts of more complex glycolipids was also found. Acid degradation and gas chromatography of the native fraction revealed fucose, glucose, galactose, N-acetylglucosamine and N-acetylgalactosamine. This is the first chemical isolation and characterization of complex blood group active glycolipids in human kidney. The existence of these molecules is discussed in view of their possible role as transplantation antigens.
D000017:ABO Blood-Group System; D002241:Carbohydrates / Q000032:analysis; D006017:Glycolipids / Q000032:analysis*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007668:Kidney / Q000276:immunology*; D007983:Lewis Blood Group Antigens; D013058:Mass Spectrometry
D000017:ABO Blood-Group System; D002241:Carbohydrates; D006017:Glycolipids; D007983:Lewis Blood Group Antigens