The effect of mixed inhibitors of cyclo-oxygenase and lipoxygenase on the indomethacin-induced hyper-reactivity in the isolated trachea of the pig.
1 Indomethacin enhances muscle contraction to histamine in the pig isolated trachea. The effect of three cyclo-oxygenase/lipoxygenase inhibitors on these potentiated histamine-induced responses was examined. 2 At the higher concentrations BW 755c (113-226 microM), 5, 8, 11, 14-eicosatetraynoic acid (ETYA, 10-100 microM) and phenidone (5-500 microM) abolished the effect of indomethacin on tracheal contractions to histamine (100 microM). 3 Each of the three inhibitors also reduced the slope of the histamine concentration-response curve obtained in the presence of indomethacin. However, the sensitivity (pD2) of the smooth muscle to histamine was not affected by BW 755c, ETYA or phenidone. 4 The results provide further support for a lipoxygenase-dependent component of indomethacin-induced hyper-reactivity in the pig trachea.
D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D000403:Airway Resistance / Q000187:drug effects*; D000818:Animals; D016861:Cyclooxygenase Inhibitors; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology*; D016859:Lipoxygenase Inhibitors; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D011720:Pyrazoles / Q000494:pharmacology; D013552:Swine; D014132:Trachea / Q000187:drug effects
D016861:Cyclooxygenase Inhibitors; D016859:Lipoxygenase Inhibitors; D011720:Pyrazoles; D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D006632:Histamine; C015429:phenidone; D007213:Indomethacin