Cell morphology, cell filaments and cell death during in vitro ageing: aphidicolin and serum deprivation effects on mouse diploid fibroblasts (a correlated scanning electron microscope and immunocytochemical study).
Terminal fibroblasts differ from early ones by slowing down in replicative activity, increase in cell area, changes in the cytoskeleton and changes in the way of dying. Replication was inhibited in early fibroblasts either with aphidicolin or by serum deprivation. Both treatments induce cell flattening and increase in cell surface. Aphidicolin has no effect on actin-microfilaments whereas serum deprivation induces the terminal pattern of filaments in many early cells. After both treatments, fibroblasts keep dying as early cells do. We conclude that there is no direct relation between changes in the organization of cell filaments and slowing down of replication (inducing cell flattening); we suggest that they depend on changes in the cell membrane structure appearing during in vitro terminal differentiation.
D000818:Animals; D016590:Aphidicolin; D002453:Cell Cycle / Q000187:drug effects*; D003470:Culture Media; D003599:Cytoskeleton / Q000648:ultrastructure; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors*; D004224:Diterpenes / Q000494:pharmacology*; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning / Q000379:methods; D019384:Nucleic Acid Synthesis Inhibitors
D003470:Culture Media; D004224:Diterpenes; D019384:Nucleic Acid Synthesis Inhibitors; D016590:Aphidicolin; D004257:DNA Polymerase II