Determination of immunoglobulin A in saliva by immunobead enzyme-linked immunosorbent assay: comparison with single radial immunodiffusion.
For the quantitative measurement of immunoglobulin A in human saliva, an immunobead enzyme-linked immunosorbent assay was investigated in detail. The method proved to be fast and sensitive, and it gave reproducible results. There was a strong correlation with results obtained by a single radial immunodiffusion method. The immunobead method gave 1.8 times higher values than the single radial immunodiffusion method with a lower correction factor for low-immunoglobulin A saliva and a higher factor for immunoglobulin A-rich saliva.
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis*; D008875:Middle Aged; D012463:Saliva / Q000276:immunology*
D007070:Immunoglobulin A