Modified enrichment broth for isolation of Yersinia enterocolitica from nonfood sources.
Sorbitol-bile salts broth was modified by the addition of 0.5% peptone for the enrichment of Yersinia enterocolitica in nonfood samples. A quantitative comparison was made of the growth of Y. enterocolitica in sorbitol-bile salts broth and peptone-sorbitol-bile salts broth at 22 degrees C. Incubation for 48 h resulted in an average 7.79 log10 increase in a viable cell number for six serotypes with peptone-sorbitol-bile salts broth compared with an average 1.85 log10 increase for sorbitol-bile salts broth. Recovery from seeded seawater showed a greater than 100-fold increase in sensitivity with peptone-sorbitol-bile salts broth enrichment. Isolation of indigenous Y. enterocolitica from fresh and marine waters was also significantly enhanced.
D001647:Bile Acids and Salts; D003470:Culture Media; D010461:Peptones; D012623:Seawater; D013012:Sorbitol; D014871:Water Microbiology; D015008:Yersinia enterocolitica / Q000254:growth & development / Q000302:isolation & purification*
D001647:Bile Acids and Salts; D003470:Culture Media; D010461:Peptones; D013012:Sorbitol