The role of cardiolipin as an acyl donor in dog heart N-acylethanolamine phospholipid biosynthesis.
N-Acylethanolamine phospholipids were produced from endogenous substrates with dog heart mitochondrial and microsomal preparations. With mitochondria the N-acyl group contained 13.8% linoleate, with microsomes only 3.6%. Cardiolipin comprised 18.5% of mitochondrial and 3.3% of microsomal lipid P and contained 93.7 and 72.4% linoleic acid, respectively. Incubation of dog heart subcellular fractions with [1-14C]linoleoyl cardiolipin in the presence of Ca2+ resulted in the formation of N-acylethanolamine phospholipids labeled primarily in the N-acyl and 1-O-acyl moieties. The data indicate that cardiolipin is the major source of linoleic acid used in the N-acylation of ethanolamine phospholipids by transacylase activity.
D000215:Acylation; D000818:Animals; D001665:Binding Sites; D002308:Cardiolipins / Q000378:metabolism*; D004285:Dogs; D004983:Ethanolamines / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008861:Microsomes / Q000378:metabolism; D008929:Mitochondria, Heart / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis*
D002308:Cardiolipins; D004983:Ethanolamines; C022203:N-acylethanolamines; D010743:Phospholipids