title: Dissociation of apolipoprotein AI from apoprotein-lipid complexes and from high-density lipoproteins. A fluorescence study.
abstract: The dissociation of the apoprotein AI (apoAI) from the apoAI-dimyristoylglycerophosphocholine complex and from human high-density lipoproteins (HDL), was induced by incubation with guanidinium hydrochloride at concentrations between 0 M and 7 M. The kinetics and extent of denaturation were followed by monitoring both the fluidity of the lipid phase by fluorescence polarization measurements and the protein conformation by measuring the tryptophanyl fluorescence emission. The association with lipids protects apoAI against denaturation both in HDL and in the apoAI-phospholipid complex. The results of the kinetic and end-point measurements suggest that the denaturing effect of guanidine hydrochloride on the apoAI-lipid complex and on HDL is a two-step process. It involves the dissociation of the apoAI-phospholipid bond, as evidenced by fluidity measurements: this effect is maximal between 3 M and 4 M guanidine hydrochloride. The conformational change of the apoAI protein into a randomly coiled structure with the tryptophanyl residues exposed to the solvent is maximal between 4 M and 6 M guanidine hydrochloride. HDL and the apoAI-phospholipid complex have a closely similar behaviour towards denaturation by guanidine hydrochloride indicating that the phospholipid-apoAI association in HDL is primarily responsible for the stability of the lipoprotein molecule.
mesh_terms: D016632:Apolipoprotein A-I; D001053:Apolipoproteins / Q000097:blood*; D004134:Dimyristoylphosphatidylcholine; D019791:Guanidine; D006146:Guanidines; D006801:Humans; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000097:blood*; D010713:Phosphatidylcholines; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence; D014364:Tryptophan
chemical_list: D016632:Apolipoprotein A-I; D001053:Apolipoproteins; D006146:Guanidines; D008077:Lipoproteins, LDL; D010713:Phosphatidylcholines; D014364:Tryptophan; D019791:Guanidine; D004134:Dimyristoylphosphatidylcholine