Gel filtration in sodium dodecyl sulphate of hydrophobic muscle proteins on Sephacryl S-400 Superfine.
Gel filtration has been applied to muscle proteins in the presence of the detergent sodium dodecyl sulphate (SDS). This detergent not only solubilizes a variety of proteins but also increases their hydrodynamic volumes, which is a disadvantage for the process of gel filtration. Nevertheless, appropriate choice of gel type, in this case Sephacryl S-400 Superfine, and optimal elution conditions make SDS gel filtration a useful tool in the separation of heterogeneous protein mixtures in the molecular weight range of 20,000-200,000.
D000180:Acrylic Resins; D000818:Animals; D002850:Chromatography, Gel; D004586:Electrophoresis / Q000379:methods; D009124:Muscle Proteins / Q000032:analysis*; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate
D000180:Acrylic Resins; D009124:Muscle Proteins; C037239:Sephacryl Superfine; D012967:Sodium Dodecyl Sulfate