Preparation of type-specific antimyosin antibodies and determination of their specificity by biochemical and immunohistochemical methods.
This paper reports the preparation of specific anti-slow myosin antibodies (anti-I) and anti-fast myosin antibodies (anti-IIA) raised against myosins from sheep and guinea pig masseter muscles. The specificity of the antibodies has been studied by immunodiffusion in agar and by the GEDELISA test using slow-twitch (type I), fast-twitch red (type IIA) and fast-twitch white (type IIB) myofibrils isolated from guinea pig muscles. The principal specificity of the anti-I and anti-IIA antibodies was for the heavy chains of type I and IIA myosins, respectively. A smaller reaction with the corresponding light chains was also detected. Immunohistochemical staining of muscle sections using these antibodies confirmed their fibre type specificity.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D002415:Cats; D002520:Cercopithecidae; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D006651:Histocytochemistry; D007120:Immunochemistry; D005779:Immunodiffusion; D009218:Myosins / Q000276:immunology*; D011817:Rabbits; D012756:Sheep
D009218:Myosins