title: Tissue distribution and ontogenetic emergence of differentiation antigens on avian T cells.
abstract: Two distinct antibodies raised against quail thymocytes were used in an indirect immunofluorescence assay to analyze the expression of differentiation antigens on T lineage cells in embryonic and newly hatched quail. (a) A rabbit serum, anti-AT-65, that has been previously shown to immunoprecipitate a protein of apparently 65-70 kDa from quail and chick thymocytes, reacts with both thymic and peripheral T cells. The polyspecificity of this pan-avian T cell reagent can be dissected by absorption and includes reactivities against two distinct antigenic entities. One of these is restricted to thymocytes whereas the other is also expressed on peripheral T cells. (b) A monoclonal antibody, a-TD1, defines the population of cortical thymocytes and does not react with peripheral T cells. Binding assays during the embryonic development of the T lineage reveal that specific surface markers are expressed 36 h following the seeding of lymphoid precursor cells to the thymic rudiment, suggesting their early commitment in this lymphoid microenvironment. T cells are also shown to be exported precociously to the embryonic splenic compartment.
mesh_terms: D000367:Age Factors; D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D002642:Chick Embryo; D004625:Embryo, Nonmammalian / Q000276:immunology; D051379:Mice; D011784:Quail / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface