Interaction of liposomes with human leukocytes in whole blood.
The uptake of multilamellar liposomes into human leukocytes in whole blood in vitro was evaluated on the basis of the cellular association of liposomal markers (3H-labelled cholesterol, lipid phase; [14C]inulin, aqueous phase). The entry of liposomes into human blood leukocytes was linear for 60 min and was mediated by a saturable mechanism displaying affinity constants of 0.28 +/- 0.17 and 0.16 +/- 0.05 mM liposomal lipid (means +/- S.E.) for liposomal lipid and aqueous phase markers, respectively. Amicon filtration analysis of incubation mixtures containing blood and liposomes (phosphatidylcholine:dicetyl phosphate:cholesterol, 70:20:10) showed that 34% of [14C]inulin was lost (neither liposome-associated nor cell-associated) after 60 min. By preincorporating sphingomyelin (35 mol%) into multilamellar liposomes, the leakage of the model aqueous phase marker inulin was reduced to 8% after 60 min, thus enhancing the drug carrier potential of liposomes in blood. As a consequence of their interaction with liposomes, the polymorphonuclear leukocytes in whole blood decreased in apparent buoyant density, while maintaining their viability. These results indicate that blood leukocytes in their natural milieu of whole blood are capable of interacting with, and taking up multilamellar liposomes.
D001693:Biological Transport, Active; D001769:Blood / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007444:Inulin / Q000097:blood; D007700:Kinetics; D007962:Leukocytes / Q000378:metabolism*; D008081:Liposomes / Q000008:administration & dosage / Q000378:metabolism*; D014677:Pharmaceutical Vehicles
D008081:Liposomes; D014677:Pharmaceutical Vehicles; D007444:Inulin