The egg-embryo chamber for intraabdominal culture.
We have developed a novel semipermeable chamber for intraabdominal culture of primate gametes and embryos. Application of the egg-embryo chamber offers a unique opportunity to study fertilization and preimplantation embryogenesis. Intrinsically normal monkey embryos displayed timely cleavage and symmetric form when cultured in this intraabdominal chamber for up to 72 hours. The birth of the first normal live monkey infant derived from the egg-embryo chamber is reported.
D000005:Abdomen; D000818:Animals; D002478:Cells, Cultured; D046508:Culture Techniques / Q000295:instrumentation*; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D000882:Haplorhini; D010063:Ovum / Q000166:cytology*