title: Regulation of HIS4-lacZ fusions in Saccharomyces cerevisiae.
abstract: The beginning of the Saccharomyces cerevisiae HIS4 gene has been fused to the structural gene for Escherichia coli beta-galactosidase. This construction, which contains HIS4 DNA from -732 to +30 relative to the translation initiation codon, has been integrated into the yeast genome at two chromosomal locations, HIS4 and URA3. At both locations, this 762-base-pair stretch of DNA is sufficient for initiating expression of beta-galactosidase activity in S. cerevisiae and confers upon this activity the regulatory response normally found for HIS4.
mesh_terms: D001483:Base Sequence; D004926:Escherichia coli; D005786:Gene Expression Regulation; D005818:Genetic Engineering; D006639:Histidine / Q000235:genetics; D007763:Lac Operon; D009876:Operon; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D006639:Histidine; D001616:beta-Galactosidase