Fidelity in transcription assessed by DNA-dependent RNA polymerases.
The use of in vitro transcription of polynucleotides by prokaryotic and eukaryotic DNA-dependent RNA polymerases has proven of great usefulness in detecting mutagenic base modifications. Quite similar results are obtained with ribo- and deoxyribopolymers as templates, and with Mg2+ and Mn2+ at optimal concentrations. Recent studies have centered on polymers containing etheno C, as well as its hydrated form. These modifications are of particular interest, since they are the consequence of metabolised vinyl chloride, a proven human carcinogen.
D000818:Animals; D002417:Cattle; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D013698:Templates, Genetic; D013950:Thymus Gland / Q000201:enzymology*; D014158:Transcription, Genetic / Q000187:drug effects
D009153:Mutagens; D008345:Manganese; D012321:DNA-Directed RNA Polymerases; D008274:Magnesium