The interrelation between high- and low-molecular-weight forms of GM1-beta-galactosidase purified from porcine spleen.
1) Two forms of acid beta-galactosidase [EC 3.1.23] with different molecular weights catalyzing the hydrolysis of GM1-ganglioside and p-nitrophenyl-beta-D-galactoside were separated and purified from porcine spleen. 2) The apparent molecular weights were 400,000-600,000 and 70,000-74,000 for the high (termed Am form) and low (termed A1 form) molecular weight forms, respectively. 3) On examination by sodium dodecyl sulfate (SDS)/polyacrylamide gel electrophoresis, both forms of the enzyme had a common protein band of molecular weight 63,000, and the Am form showed three additional protein bands with molecular weights of 31,000, 21,000, and 20,000. 4) Both forms of the enzyme had similar catalytic functions with regard to pH-optimum, Km, substrate specificity and sensitivity to substrate analogues and other substances such as detergents, bovine serum albumin (BSA) and NaCl. 5) Both forms of the enzyme were fairly stable upon preincubation at 45 degrees C at acidic pH (pH 4.5), but lost their activities at neutral pH (pH 7.0). 6) The A1 form was a monomer at neutral pH (pH 7.0) and formed a dimer at acidic pH (pH 4.5). However, most of the Am form could not be converted to a dimeric form on gel filtration at acidic pH.
D000818:Animals; D005696:Galactosidases / Q000302:isolation & purification*; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D013154:Spleen / Q000201:enzymology*; D013379:Substrate Specificity; D013552:Swine; D001616:beta-Galactosidase / Q000302:isolation & purification*
D046911:Macromolecular Substances; D005696:Galactosidases; C021502:acid beta-galactosidase; D001616:beta-Galactosidase