The induction of glial proliferation by an astrocytoma-derived growth factor resembling glia maturation factor.
Glia maturation factor (GMF)-like activity which induces DNA synthesis and morphological differentiation of density-inhibited glioblasts was detected in various glial tumor cells. A polypeptide from C6 cells (rat astrocytoma) which has a molecular weight range of 40,000-50,000 showed the highest activity. This factor also induced DNA synthesis in glioma cells (354A and LRM55) and fibroblast (Swiss 3T3). The activity was susceptible to heat treatment at 70 degrees C for 5 min, or to proteases such as trypsin, chymotrypsin, papain, and subtilisin, but it was devoid of esteropeptidase activity. The isoelectric point was found to be 5.3. Subcellular fractionation localized the activity in cytosomal and microsomal fractions. These properties closely resemble those of GMF from pig and bovine brain.
D000818:Animals; D001254:Astrocytoma / Q000503:physiopathology*; D002455:Cell Division; D002460:Cell Line; D004950:Esterases / Q000378:metabolism; D020904:Glia Maturation Factor; D005910:Glioma / Q000473:pathology / Q000503:physiopathology*; D006133:Growth Substances / Q000502:physiology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009363:Neoplasm Proteins / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000502:physiology*; D009457:Neuroglia / Q000502:physiology*; D010447:Peptide Hydrolases; D051381:Rats; D001617:beta-Glucosidase / Q000378:metabolism
D020904:Glia Maturation Factor; D006133:Growth Substances; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D007770:L-Lactate Dehydrogenase; D004950:Esterases; D001617:beta-Glucosidase; D010447:Peptide Hydrolases