Isolation, purification and characterization of spleen ferritin of Gallus domesticus L.
The ferritin from the spleen of the chickens has been isolated by a method of salt fractionation and by a pH change followed by purification in sephadex G-200. 2. The identification of the protein was carried out by acrylamide gel electrophoresis showing a single band. 3. The characterization of ferritin has been made by determination of molecular weight, amino acids analysis and the number of iron atoms (4520) which bound the ferritin. 4. The ferritin from the spleen of chicken is compared with the ferritin from the liver of pigeon.
D000418:Albumins; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002374:Catalase; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens / Q000378:metabolism*; D005293:Ferritins / Q000302:isolation & purification*; D007770:L-Lactate Dehydrogenase; D008970:Molecular Weight; D013154:Spleen / Q000032:analysis*
D000418:Albumins; D000596:Amino Acids; D005293:Ferritins; D007770:L-Lactate Dehydrogenase; D002374:Catalase