title: Proton magnetic resonance melting studies of CCGGp, CCGGAp, ACCGGp, CCGGUp, and ACCGGUp.
abstract: Proton magnetic resonance melting curves are reported for the base and ribose 1' nonexchangeable protons of CCGGp, CCGGAp, ACCGGp, CCGGUp, and ACCGGUp. In general, the double helices melt in a cooperative two-state transition. For CCGGp, CCGGAp, CCGGUp, and ACCGGUp, the 3' ribose exhibits conformational flexibility at lower temperatures than the other ribose residues. For ACCGGp, the 5'-C ribose is the first to exhibit flexibility. The conformations of the 3' terminal bases in CCGGAp and CCGGUp change cooperatively with the double- to single-strand transition, whereas the 5' A of ACCGGp appears to have more conformational freedom.
mesh_terms: D001483:Base Sequence; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D013696:Temperature; D013816:Thermodynamics
chemical_list: D009841:Oligonucleotides; D009843:Oligoribonucleotides