Acceleration of coagulation by spectrin-free erythrocytic vesicles. Evidence for lipid flip-flop during vesicle formation.
Spectrin-free erythrocytic vesicles isolated from outdated liquid-preserved blood samples show a distinct increase of the clot-promoting activity compared with membrane phospholipid equivalents of intact erythrocytes. We suppose that, among other remodelling processes, local spectrin detachment from the inner membrane surface may trigger a flip-flop-mediated disturbance of the membrane lipid asymmetry. The interactions of spectrin with the cytoplasmic membrane surface seem to be essential for the structural membrane integrity including the lipid asymmetry.
D001777:Blood Coagulation; D001793:Blood Preservation; D004910:Erythrocyte Membrane / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis*; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D008563:Membrane Lipids; D008565:Membrane Proteins / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis; D013049:Spectrin / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology
D006023:Glycoproteins; D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids; D013049:Spectrin; D014357:Trypsin