The effects of serum and phorbol ester tumor promoter on ornithine decarboxylase activity in Swiss 3T3 cells.
Addition of the tumor promotor 12-O-tetradecanoyl phorbol-13-acetate (TPA) to serum-deprived mouse 3T3 cells resulted in an increase in ornithine decarboxylase activity which is maximal at 3 h and declined thereafter. Addition of serum to these same cells resulted in an ornithine decarboxylase (ODC) response which was identical to the TPA-induced response at 3 h but continued to increase to a maximum at 6 h. A variant line of 3T3 cells, which possessed phorbol ester receptors, failed to increase its ODC in response to TPA. Also, the early component of the serum-induced ODC response was markedly diminished. These data suggest that phorbol ester tumor promoters may induce an early component of a more extensive ODC response, and that a component of serum may act through the same pathway as the phorbol esters.
D000818:Animals; D001769:Blood; D002262:Carboxy-Lyases / Q000378:metabolism*; D002478:Cells, Cultured; D051379:Mice; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010704:Phorbols / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D010704:Phorbols; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate