title: A tracheal culture model of respiratory tract infection with Pseudomonas aeruginosa.
abstract: The pathogenesis of Pseudomonas aeruginosa for the respiratory tract has been examined using hamster tracheal organ cultures. Tracheal rings prepared from male Syrian hamsters, strain LSH/LAK, were infected with P. aeruginosa for 4 h and processed at 4-h intervals for 24 h for examination by light- and electron microscopy. Tissue destruction was observed within 8 h after infection with 10(8) colony-forming units (cfu)/ml and within 12 h after infection with 10(4) or 10(6) cfu/ml. Ciliated cells that contained abnormal subcellular organelles were expelled from the epithelium. By 20 h the epithelial borders were composed primarily of nonciliated cells. Transmission- and scanning electron microscopy revealed details of the cellular destruction and attachment of P. aeruginosa to the ciliated epithelium. Pseudomonas aeruginosa causes a rapid destruction of the epithelium of hamster trachea in cultures. Hamster tracheal organ cultures have been shown to be useful in studying the pathogenesis of P. aeruginosa for the respiratory tract.
mesh_terms: D000818:Animals; D006224:Cricetinae; D004195:Disease Models, Animal; D008297:Male; D008647:Mesocricetus; D009924:Organ Culture Techniques; D011552:Pseudomonas Infections / Q000473:pathology*; D011550:Pseudomonas aeruginosa; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000473:pathology*; D014132:Trachea / Q000382:microbiology* / Q000473:pathology