Electromicroelution and microfingerprinting of radiolabeled immunoglobulins from sodium dodecyl sulfate-polyacrylamide gels.
An apparatus for electromicroelution from gel slices is described. After 20 min 70-90% of radiolabeled molecules can be extracted from the gel and recovered in 100 mul of buffer. The extracted molecules could be analysed in SDS-PAGE after reduction or could be split to perform microfingerprints (4 cm X 4 cm). This method has been used in several studies on membrane molecules (Fc gamma receptor, surface IgD, T cell receptor).
D000818:Animals; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007136:Immunoglobulins; D007158:Immunologic Techniques / Q000295:instrumentation*; D051379:Mice; D008832:Microchemistry; D012967:Sodium Dodecyl Sulfate
D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007136:Immunoglobulins; D012967:Sodium Dodecyl Sulfate