title: Structural studies of cytochrome P-450 using small angle x-ray scattering.
abstract: We have employed small angle x-ray scattering to examine the solution conformation of cytochrome P-450cam in the presence and absence of its substrate, camphor. The radius of gyration (Rg) of the protein is found to be 23.9 +/- 0.2 A in both states. Comparison of the radius of gyration with that expected for a spherical protein of the same molecular weight indicates that P-450 is an elongated molecule and that its shape may best be approximated by a cylinder about 30 A in diameter and 80 A in length.
mesh_terms: D003577:Cytochrome P-450 Enzyme System; D011487:Protein Conformation; D011549:Pseudomonas / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D003577:Cytochrome P-450 Enzyme System