title: Effect of amino-acids on secretion of insulin by isolated islets of the monkey.
abstract: Insulin secretion was monitored in monkey islets isolated by collagenase digestion and exposed to leucine and arginine with and without glucose. Leucine by itself (10 to 40 mmol/l) elicited concentration-dependent insulin secretion. At 40 mmol/l, leucine was approximately 60% as effective as glucose (16.7 mmol/l). The response to leucine was increased at low glucose concentrations. In high concentrations (20 and 40 mmol/l), arginine by itself was a poor stimulant. The effect of arginine was enhanced at low glucose concentrations (2.8 to 5.6 mmol/l). At high glucose concentrations neither amino-acid produced any significant further increase in insulin release.
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology; D000882:Haplorhini; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D007930:Leucine / Q000494:pharmacology*
chemical_list: D007328:Insulin; D001120:Arginine; D007930:Leucine; D005947:Glucose