Bioflavonoid-mediated stabilization of collagen in adjuvant-induced arthritis.
In rats with adjuvant-induced arthritis, the effect of (+)-catechin (CA) and 0-(beta-hydroxyethyl) rutosides (HR) on the crosslinking of collagen was studied. Compared with controls the arthritic group showed an increase in the reversibility of neutral salt-soluble collagen gel, solubility of acid-insoluble collagen to denaturing agents and the ratio of alpha/beta subunits of neutral salt-soluble collagen. These results suggest an impaired maturation of collagen in arthritic animals. Administration of CA or HR to the arthritic animals caused a decrease in the reversibility of collagen gel and in the solubility of acid-insoluble collagen to denaturing agents and to pronase. In addition, the electrophoretic patterns of neutral salt-soluble collagen on SDS polyacrylamide gels also showed a decrease in the alpha/beta ratio. All these results may collectively indicate that both flavonoids promote the crosslinking of collagen in arthritic animals.
D000818:Animals; D001168:Arthritis / Q000378:metabolism*; D001169:Arthritis, Experimental / Q000378:metabolism*; D002392:Catechin / Q000494:pharmacology; D003094:Collagen / Q000378:metabolism*; D005419:Flavonoids / Q000494:pharmacology*; D006896:Hydroxyethylrutoside / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D012867:Skin / Q000378:metabolism
D005419:Flavonoids; D006896:Hydroxyethylrutoside; D002392:Catechin; D003094:Collagen