Selective defect of natural killer and killer cell activity against lymphomas in SJL mice: low responsiveness to interferon inducers.
Three unrelated inbred strains of H-2s mice, SJL/JN, A.SW, and B10.S, were classified according to the levels of splenic NK activity against susceptible lymphoma targets, with or without prior administration of the interferon (IFN) inducer p-I:C to the prospective donors of splenocytes: 1) SJL/JN had low endogenous activity that responded poorly to p-I:C-induced augmentation; 2) A.SW exhibited low levels of spontaneous activity that were inducible to higher levels by p-I:C; and 3) B10.S showed high endogenous levels of activity that were augmented to a still higher level by p-I:C treatment. In vitro incubation of spleen cells with partially purified IFN-beta for up to 12 hr augmented NK activity of A.SW and B10.S but not of SJL mice. The relative levels of K cell-mediated ADCC in the three strains were similar to those of NK activity, including augmentability by p-I:C; however, the capacity to perform three additional cell-mediated immune functions, i.e., natural cytotoxicity against a fibrosarcoma target, adherent cell-mediated ADCC against antibody-coated chicken RBC, and alloimmune T cell-mediated cytotoxicity against appropriate targets, did not distinguish one strain from another. The low NK phenotype in SJL/JN remained distinctive throughout postnatal life, but there was a tendency for the highest levels of NK activity to occur in 4 to 8-wk-old mice.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D002645:Chickens; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006183:H-2 Antigens / Q000235:genetics; D007369:Interferon Inducers / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology*; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D010641:Phenotype; D013154:Spleen / Q000166:cytology
D006183:H-2 Antigens; D007369:Interferon Inducers