Homo- and heterogeneous forms of human factor VIII.
In order to determine the molecular size of human F VIII as found in fresh plasma and as found in fresh-frozen plasma and in cryoprecipitate and to study the relationship of the factor-related properties, namely F VIII:C, F VIIR:Rcof and F VIIIR:Ag to F VIII, the factor's elution pattern on Sephacryl 1000 was investigated. When fresh plasma was chromatographed on this gel, F VIII eluted in a single sharp peak with all three F VIII-related activities appearing in the separation range of the gel column. The molecular weight of F VIII was calculated to be higher than 8 X 10(5). When fresh-frozen plasma was chromatographed on this gel, the elution pattern was identical to that of fresh plasma with the single exception that in several samples F VIII eluted in a broader peak tailing to higher molecular forms. When cryoglobulin fraction of fresh-frozen plasma was chromatographed, F VIII showed distinct heterogenicity in the elution pattern with respect to molecular size and relationship to the F VIII-related properties. Chelation of the endogenous plasma Ca-ions by citrate was found to have no influence on the factor's elution pattern.
D000818:Animals; D000941:Antigens / Q000032:analysis; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D003450:Cryoglobulins; D005169:Factor VIII / Q000032:analysis / Q000276:immunology; D005615:Freezing; D006801:Humans; D008970:Molecular Weight; D011817:Rabbits; D012310:Ristocetin / Q000032:analysis; D014841:von Willebrand Factor
D000941:Antigens; D003450:Cryoglobulins; D014841:von Willebrand Factor; D012310:Ristocetin; D005169:Factor VIII