Ribosome purification by isopycnic sedimentation in high density sucrose gradients.
The applicability has been investigated of a method for the isolation of cytoplasm (and mitochondrial) ribosomes of various eukaryotes. The procedure is based on centrifugation techniques and includes a sedimentation of the ribosomes in a sucrose gradient of very high density. It was found to be particularly useful with material which contains relatively few ribosomes.
D000818:Animals; D001156:Artemia; D002458:Cell Fractionation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D002645:Chickens; D004175:Diptera; D004625:Embryo, Nonmammalian; D005056:Euglena gracilis / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008099:Liver / Q000648:ultrastructure; D009835:Oligochaeta; D010920:Placenta / Q000648:ultrastructure; D011247:Pregnancy; D012270:Ribosomes / Q000648:ultrastructure*; D013769:Tetrahymena pyriformis / Q000648:ultrastructure