Steroidogenic and spermatogenic potentials of the male rat after acute treatment with aflatoxin B1.
150 microgram of aflatoxin B1 were injected i.p]. to male rats on three occasions at intervals 48 h. Sperm production and plasma and testicular testosterone concentrations were determined in rats killed 24 h after the last dose of aflatoxin with or without stimulation with human chorionic gonadotropin (HCG). Neither sperm production nor testosterone secreting capacity was significantly depressed by the acute aflatoxin treatment. The Leydig cell function was however impaired as indicated by the extremely reduced responsiveness of the Leydig cells of the aflatoxin-pretreated rats to HCG stimulation. It may be concluded that the disruption of spermatogenesis by chronic aflatoxin treatment is preceded and caused by the impairment of the Leydig cell function and the resultant drop in testosterone in the testis.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000494:pharmacology*; D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D007985:Leydig Cells / Q000187:drug effects*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000032:analysis; D013739:Testosterone / Q000097:blood
D000348:Aflatoxins; D006063:Chorionic Gonadotropin; D013739:Testosterone; D016604:Aflatoxin B1