Analysis of the acid polysaccharides from squid cranial cartilage and examination of a novel polysaccharide.
The polysaccharides of cranical cartilage were isolated by ethanol precipitation after papain digestion and beta-elimination procedures and were fractionated chromatographically on CPC-cellulose. In addition to the previously described, heavily oversulphated chondroitin sulphate, the tissue contained small amounts of hyaluronic acid, which, however, co-eluted with the chondroitin sulphate from the CPC-cellulose. Approx. 20% of the isolated polysaccharides consisted of an acidic polysaccharide which to our knowledge is not previously described. This polysaccharide consists mainly of glucuronic acid, galactose and mannose in a molar ratio of 1:2:1. Gel chromatography of the preparation indicated a polydisperse molecule with an apparent average molecular weight of 39 200 on weight basis (Mw) and 31 400 on number basis (Mn).
D000818:Animals; D002356:Cartilage / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D049832:Decapodiformes / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D011134:Polysaccharides / Q000302:isolation & purification*; D012886:Skull / Q000032:analysis
D011134:Polysaccharides