The effect of glutathione conjugation and microsomal oxidation on the mutagenicity of dichloromethane in S. typhimurium.
The effect of mammalian metabolizing enzymes on the mutagenicity of dichloromethane was investigated in S. typhimurium strain TA100. The compound was directly mutagenic towards this strain, but the mutagenic activity was enhanced upon addition of either rat-liver microsomes or the cytosol fraction. Further experiments showed that both the cytochrome P450 oxygenases and the glutathione-S-transferases are involved in the bioactivation of dichloromethane.
D000818:Animals; D001711:Biotransformation; D003600:Cytosol / Q000378:metabolism; D005982:Glutathione Transferase / Q000494:pharmacology; D006843:Hydrocarbons, Chlorinated / Q000494:pharmacology*; D008752:Methylene Chloride / Q000378:metabolism / Q000494:pharmacology*; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens; D009251:NADPH-Ferrihemoprotein Reductase / Q000494:pharmacology; D051381:Rats; D012486:Salmonella typhimurium / Q000235:genetics
D006843:Hydrocarbons, Chlorinated; D009153:Mutagens; D008752:Methylene Chloride; D009251:NADPH-Ferrihemoprotein Reductase; D005982:Glutathione Transferase