Evolution of tubulin heterogeneity during mouse brain development.
In this report, we have characterized tubulin subunit heterogeneity and its evolution during mouse brain development, from embryonic to adult stages. A modification of the two-dimensional protein analysis was used to specify these events. The number of isotubulins increases from 6 (4 alpha and 2 beta), in the embryonic brain, to 11 (6 alpha and 5 beta), in the adult. The changes occurring in tubulin heterogeneity are developmentally controlled but it seems that alpha and beta isotubulins are independently regulated: changes in alpha tubulin occur only just before birth whereas the major evolution is concerned with the appearance and accumulation of acidic beta isotubulins throughout development.
D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007525:Isoelectric Focusing; D046911:Macromolecular Substances; D051379:Mice; D011247:Pregnancy; D014404:Tubulin / Q000378:metabolism*
D046911:Macromolecular Substances; D014404:Tubulin