Primary structure of a novel N-glycosidic carbohydrate unit, derived from hen ovomucoid. A 500-MHz 1H-NMR study.
The N-glycosidic carbohydrate chains of hen beta-ovomucoid were released from the protein by hydrazinolysis, and separated by HPLC. Primary structural analysis of 3 major fractions was conducted by applying 500-MHz 1H-NMR spectroscopy in combination with methylation analysis. One of the fractions investigated appeared to consist of an intersected penta-antennary structure extended with one Gal residue. The location of the latter in a certain branch could be established unambiguously by NMR. This structure is a novel member of the family of N-glycosidic carbohydrates of glycoproteins.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002627:Chemistry, Physical; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004527:Egg Proteins; D005260:Female; D006027:Glycosides / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D010059:Ovomucin
D004527:Egg Proteins; D006027:Glycosides; D010059:Ovomucin