Cloning the gentamicin resistance gene from a Pseudomonas aeruginosa plasmid in Escherichia coli enhances detection of aminoglycoside modification.
Cloning the gene for gentamicin resistance from Pseudomonas aeruginosa plasmid pMG35 on the high-copy-number Escherichia coli cloning vehicle pMK20 allowed detection of 6'-N-acetyltransferase activity that was not readily detected when the parent plasmid was present either in P. aeruginosa or E. coli.
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005839:Gentamicins / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics*; D011815:R Factors
D005839:Gentamicins