Comparative effect of lipoxygenase products of arachidonic acid on rat aortic smooth muscle cell migration.
We investigated the effects of mono-hydroxyeicosatetraenoic acids (HETEs) and N-formyl-methionyl-leucyl-phenylalanine (F-Met-Leu-Phe) on rat aortic smooth muscle cell migration in modified Boyden chambers. 12-HETE showed the most potent stimulatory effect on smooth muscle cell migration among the mono-HETEs tested. The optimal concentrations for cell migration were 3 X 10(-15) and 3 X 10(-13) g/ml for 12-HETE and 10(-8) g/ml for 15-HETE, 5-HETE and F-Met-Leu-Phe were inactive with these cells. As 12-HETE is biosynthesized from arachidonic acid by the 12-lipoxygenase pathway in platelets and macrophages, and 15-HETE by the 15-lipoxygenase pathway in granulocytes, the present results indicate an important role for such cells in the early phase of atherosclerosis.
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D001011:Aorta / Q000502:physiology*; D001091:Arachidonate Lipoxygenases; D001095:Arachidonic Acids / Q000096:biosynthesis / Q000494:pharmacology*; D001161:Arteriosclerosis / Q000209:etiology / Q000503:physiopathology; D002465:Cell Movement / Q000187:drug effects; D006098:Granulocytes / Q000378:metabolism; D006893:Hydroxyeicosatetraenoic Acids; D008084:Lipoxygenase / Q000096:biosynthesis / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000502:physiology; D009239:N-Formylmethionine / Q000031:analogs & derivatives / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009842:Oligopeptides / Q000494:pharmacology; D051381:Rats
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D009842:Oligopeptides; D009239:N-Formylmethionine; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; D009240:N-Formylmethionine Leucyl-Phenylalanine; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D001091:Arachidonate Lipoxygenases; D008084:Lipoxygenase