Human aldolase B subunit-specific radioimmunoassay.
A radioimmunoassay was developed for the direct quantification of aldolase B in human serum and tissues. The method is a double antibody radioimmunoassay technique using radioiodinated aldolase B homopolymer as ligand, chicken antibodies to aldolase B and rabbit antibodies to chicken IgG. This radioimmunoassay was shown to be specific for the aldolase B subunit, with no cross-reactivity with either human aldolase A subunit or homopolymeric human aldolase C (C4). The lowest measurable amount by this method was 2 ng/ml. Aldolase B is predominantly found in normal liver tissue, with relatively high aldolase B levels also observed in kidney. Aldolase B levels in the serum obtained from 11 normal subjects ranged from 23 to 38 ng/ml, with a mean of 28.5 +/- 9.2 (S.D.) ng/ml. Almost all of patients with hepatitis had serum aldolase B levels greater than 30 ng/ml. In cancer patients, serum aldolase B was slightly elevated in patients with metastatic liver cancer and primary lever cell carcinoma, whereas no elevation of serum aldolase B was shown in patients without liver metastasis.
D002700:Chloramines; D005634:Fructose-Bisphosphate Aldolase / Q000032:analysis* / Q000097:blood; D006801:Humans; D007527:Isoenzymes / Q000032:analysis* / Q000097:blood; D008107:Liver Diseases / Q000201:enzymology; D011863:Radioimmunoassay / Q000379:methods*; D013997:Time Factors; D014018:Tissue Distribution; D014105:Tosyl Compounds; D014508:Urea / Q000031:analogs & derivatives
D002700:Chloramines; D007527:Isoenzymes; D014105:Tosyl Compounds; C016300:chloramine-T; C012385:1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril; D014508:Urea; D005634:Fructose-Bisphosphate Aldolase