title: Location of a protein of the fodrin-spectrin-TW260/240 family in the mouse intestinal brush border.
abstract: We have determined that a protein of the fodrin-spectrin-TW260/240 (FST) family is a component of the thin fibrils (approximately 5 nm wide, 100-200 nm long) that cross-link bundles of actin filaments to adjacent actin bundles and to the plasma membrane in the terminal web of the brush border of the intestinal epithelium. When isolated brush borders were incubated with anti-fodrin antibodies and prepared for electron microscopy by the quick-freeze, deep-etch technique, these approximately 5 nm fibrils were specifically decorated with the antibody. In addition, these cross-linking fibrils disappeared when the anti-fodrin-reactive proteins were extracted from the brush border. We conclude that FST is a component of a cross-linking system composed of approximately 5 nm fibrils that are morphologically distinct from the approximately 8 nm myosin-containing fibrils which were identified by anti-myosin decoration. In addition to linking actin bundles to adjacent actin bundles and to the plasma membrane, these FST fibrils may mediate actin-vesicle, actin-intermediate filament and vesicle-plasma membrane linkages.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D002352:Carrier Proteins / Q000032:analysis*; D003429:Cross Reactions; D005614:Freeze Fracturing; D006168:Guinea Pigs; D007422:Intestines / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000032:analysis*; D051379:Mice; D008840:Microfilament Proteins; D008854:Microscopy, Electron; D008871:Microvilli / Q000032:analysis; D009218:Myosins / Q000032:analysis; D011817:Rabbits; D013049:Spectrin / Q000032:analysis*
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D009218:Myosins; D013049:Spectrin; C034037:F-actin-binding proteins