title: Characterization of the uptake and toxicity of a fluorescent thiol reagent.
abstract: 3-(4-Maleimidylphenyl)-4-methyl-7-diethylamino coumarin (CPM), is a fluorescent thiol-binding agent. CPM is nontoxic to Chinese hamster V-79 cells (2 x 10(5) cells/ milliliter) exposed to 2.5 micrograms/milliliter for 30 minutes. However, both toxicity and cellular binding were directly dependent on the drug:cell ratio. Using flow cytometry, cellular binding of CPM correlated with inhibition of DNA synthesis. In cells pretreated with the thiol binding drugs N-ethylmaleimide, diamide and diethylmaleate or the carcinogens 4-nitroquinoline-1-oxide and AF-2 (furylfuramide), the subsequent binding of CPM was reduced by 40% or more.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003374:Coumarins / Q000378:metabolism* / Q000633:toxicity; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000096:biosynthesis; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000378:metabolism* / Q000633:toxicity; D013439:Sulfhydryl Reagents / Q000378:metabolism* / Q000633:toxicity
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; D013439:Sulfhydryl Reagents; C030860:N-(4-(7-diethylamino-4-methylcoumarin-3-yl)phenyl)maleimide; D004247:DNA