[Determination of beta-mannanase activity by viscosimetric and spectrophotometric methods].
The activity of beta-mannanase from Bacillus subtilis was measured viscosimetrically and spectrophotometrically. As substrate galactomannane of Ceratonia siliqua was used. Relationships between the beta-mannanase activity and the substrate concentration as well as the enzyme content were investigated. The kinetic parameters of the enzymes obeying the Michaelis-Menten equation were calculated. It was found viscosimetrically that Vmax of the commercial enzyme preparation was 1.4 mucat/g (at pH 5.8 and 40 degrees) and Km was 0.6 mM. The viscosimetric method shows high sensitivity, whereas the spectrophotometric technique suits mass-scale analyses.
D001412:Bacillus subtilis / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D008361:Mannosidases / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods*; D014783:Viscosity; D044902:beta-Mannosidase
D008361:Mannosidases; D044902:beta-Mannosidase
Opredelenie aktivnosti beta-mannanazy viskozimetricheskim i spektrofotometricheskim metodami.