Venezuelan equine encephalomyelitis virus: concentration, partial purification, inactivation and immunogenicity.
Venezuelan equine encephalomyelitis (VEE) TC-84 vaccinal virus, from 10-1. quantities of infected duck embryo fibroblast cell culture fluids, was isolated by combined continuous-flow centrifugation with isopycnic banding in sucrose. Most of the recovered infectivity and hemagglutinating activity were in a single band at a buoyant density (rho) of 1.2. About 90% of the total input protein (450-520 mg) was removed with the effluent, whereas most of the remaining 10% also banded at a rho of 1.2. Infectivity was inactivated with formalin at a final concentration of 0.05% at 37 degrees C for 24 hr. Formalin-inactivated virus retained its immunogenicity and induced VEE virus-specific antibody in horses and guinea pigs. The horses and those guinea pigs that received equivalent doses of vaccine survived after a challenge of their immunity with virulent VEE virus.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002500:Centrifugation, Isopycnic; D004666:Encephalitis Virus, Venezuelan Equine / Q000276:immunology* / Q000302:isolation & purification; D004683:Encephalomyelitis, Equine / Q000662:veterinary*; D004685:Encephalomyelitis, Venezuelan Equine / Q000517:prevention & control / Q000662:veterinary*; D006168:Guinea Pigs; D006389:Hemagglutinins, Viral / Q000032:analysis; D006734:Horse Diseases / Q000517:prevention & control; D006736:Horses; D014613:Vaccines, Attenuated; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines