Transcription and translation in the autonomous parvovirus KRV.
We describe here the isolation and partial characterization of at least five viral specific RNAs synthesized in the rat nephroma (RN) cell after infection with the parvovirus KRV. The RNAs have the approximate lengths of 4.7, 3.4, 3.0, 1.25, and 0.95 kilobases (kb) and are probably the functional messages. The 4.7-kb RNA would represent a transcript of 95 to 100% of the viral genome. The most abundant message, about 3.0 kb, represents over 50% of the viral genome and probably codes in the reticulocyte transcribing system for the most abundant viral protein (MW 68,000) which is the main viral capsid protein. This abundant RNA, on the basis of R loop data, has an origin of transcription about 0.38 to 0.42 map units from the 3' end of the single-stranded KRV genome.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D007680:Kidney Neoplasms; D008854:Microscopy, Electron; D010321:Parvoviridae / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification; D012367:RNA, Viral / Q000302:isolation & purification; D051381:Rats; D013997:Time Factors; D014158:Transcription, Genetic
D012333:RNA, Messenger; D012367:RNA, Viral