Creatine kinase from the electric organ of Electrophorus electricus (L.)--isozyme analysis.
Creatine kinase (CPK) isozymes of extracts from the electric organ, dorsal muscle and brain of Electrophorus electricus (L.) were analysed with Cellogel electrophoresis. A single component corresponding to the MB-form was obtained for both electric organ and the dorsal muscle. The BB-form was present in the brain extract. 2. Upon acetone fractionation of the aqueous of electric organ, the final fraction was submitted to gel filtration and presented a single peak of CPK activity. 3. Characterization of this fraction by thin-layer gel filtration indicated an apparent molecular weight of 80,000 which corresponds to the enzyme dimeric structure. 4. The implications of this finding with the muscular origin of the electric organ are discussed.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003402:Creatine Kinase / Q000302:isolation & purification*; D004557:Electric Organ / Q000201:enzymology*; D004593:Electrophorus; D007527:Isoenzymes; D009928:Organ Specificity
D007527:Isoenzymes; D003402:Creatine Kinase