Selection in Bacillus subtilis giving rise to strains with mutational alterations in a variety of ribosomal proteins.
To facilitate mapping of ribosomal protein genes in Bacillus subtilis, a selection was devised which gave rise to strains with alterations in any one of a variety or ribosomal proteins. Alterations in eighteen ribosomal proteins were identified when eighty mutants were analysed. In addition, one strain showed a major assembly defect in the large ribosomal subunit resulting in the presence of a particle sedimenting at about 40S. Eighteen large subunit proteins were present on this particle in normal amounts, while twelve proteins were much reduced in amount or undetectable.
D001412:Bacillus subtilis / Q000235:genetics*; D002498:Centrifugation; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D046911:Macromolecular Substances; D008970:Molecular Weight; D009154:Mutation; D012269:Ribosomal Proteins / Q000235:genetics*; D012641:Selection, Genetic
D046911:Macromolecular Substances; D012269:Ribosomal Proteins