[Production of monospecific type A botulinum toxin and antiserum using a column chromatographic method].
A column chromatography method was employed to obtain a monospecific botulinus type A toxin. The latter was found to be a homogeneous product via column chromatographic and electrophoretic studies. The immunodiffusion test after Ouchterlony with a serum obtained with the use of a native toxin yielded one precipitation line. No such lines were produced with a serum obtained from animals immunized with a type B toxin. The purified toxin could be detoxicated by routinely applied methods. When injected to rabbits it induced the production of monospecific antibodies.
D000818:Animals; D001904:Botulinum Antitoxin; D001905:Botulinum Toxins / Q000276:immunology / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D011817:Rabbits / Q000276:immunology
D001904:Botulinum Antitoxin; D001905:Botulinum Toxins
Poluchavane na monospetsifichen butolinov toksin tip A i antiserum chrez izpolzuvane na kolonnokhromatografskiia metod.