title: Brugia malayi and B. pahangi: cultivation in vitro of infective larvae to the fourth and fifth stages.
abstract: Cultivation of fourth stage Brugia pahangi and B. malayi larvae from infective larvae (stage 3) were obtained in culture medium RPMI 1640 supplemented with 10% human AB serum and an LCC-MK2 rhesus monkey kidney continuous cell line feeder layer. This culture system kept larvae alive in excess of 7 weeks, and served as a source for collection of the worms' secretory, excretory, and moulting antigens.
mesh_terms: D000818:Animals; D001769:Blood; D002009:Brugia / Q000033:anatomy & histology / Q000254:growth & development*; D002460:Cell Line; D003470:Culture Media; D005370:Filarioidea / Q000254:growth & development*; D007814:Larva / Q000254:growth & development; D008253:Macaca mulatta; D008675:Metamorphosis, Biological; D010274:Parasitology / Q000379:methods*
chemical_list: D003470:Culture Media