Effect of selenium on the subcellular distribution of glutathione peroxidase in rat liver, epididymal fat pad and seminal vesicle.
The effect of dietary selenium on the subcellular distribution of selenium-dependent glutathione peroxidase (SeGSH-Px) activity in rat liver, epididymal fat pad, and seminal vesicle was determined. Tissues were fractionated by differential centrifugation, and the subcellular distribution of SeGSH-Px activity was determined by comparison with the distribution of biochemical marker enzymes. Liver SeGSH-Px activity was located in both the cytosol and mitochondria. In epididymal fat pad and seminal vesicle, SeGSH-Px activity was located primarily in the cytosol; association with another subcellular organelle, however, was indicated. In liver and epididymal fat pad, SeGSH-Px activity increased linearly, and, in the seminal vesicle, increased linearly and quadratically, with increasing dietary selenium concentration. Distribution of SeGSH-Px activity among the cellular fractions from the tissues, however, was not affected by dietary selenium supplementation.
D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D004247:DNA / Q000032:analysis; D004032:Diet; D004822:Epididymis / Q000201:enzymology; D005979:Glutathione Peroxidase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D010544:Peroxidases / Q000378:metabolism*; D051381:Rats; D012643:Selenium / Q000494:pharmacology*; D012669:Seminal Vesicles / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology; D014018:Tissue Distribution
D004247:DNA; D010544:Peroxidases; D005979:Glutathione Peroxidase; D012643:Selenium