Induction and rapid repair of sister-chromatid exchanges in multiple murine tissues in vivo by diepoxybutane.
Sister-chromatid exchange (SCE) induction by the direct-acting bifunctional carcinogen, diepoxybutane (DEB), was investigated in multiple tissues in vivo. The log-log dose SCE response relationship was found to be parallel to that previously reported for DEB induction of lung adenomas. However, the SCE assay is approximately 20 times as sensitive in detecting genotoxic effects of DEB than indicated by the lung adenoma assay. Examination of second and third division cells following various treatment protocols revealed that regardless of the nature of initially induced lesions, they are rapidly repaired with no evidence of persistence beyond 1 cell cycle.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003434:Crossing Over, Genetic / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004852:Epoxy Compounds / Q000494:pharmacology*; D004988:Ethers, Cyclic / Q000494:pharmacology*; D008115:Liver Regeneration; D008168:Lung / Q000166:cytology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D004852:Epoxy Compounds; D004988:Ethers, Cyclic; C007366:diepoxybutane