Incorporation of radioactive 35SO4(2-) into immunoreactive pituitary lutropin.
The terminal hexosamines of bovine pituitary lutropin are thought to contain a sulfate moiety. In order to test this, a biosynthetic approach was adopted. When rat and buffalo (bovine) pituitaries were incubated with radioactive 35SO4(2-) for 2 h in vitro it was observed that radioactivity test incorporated into trichloroacetic acid-precipitable proteins. When the radioactive proteins were treated with an anti-sheep lutropin serum, radioactivity was found in the immunoprecipitate. The incorporation into rat lutropin like material was very marginal while it was very significant in the case of buffalo lutropin.
D000818:Animals; D002020:Buffaloes; D007986:Luteinizing Hormone / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D012756:Sheep; D013045:Species Specificity; D013431:Sulfates / Q000378:metabolism*
D013431:Sulfates; D007986:Luteinizing Hormone