Antibodies to Brucella abortus in sera from Strain 19 vaccinated and non-vaccinated cows as determined by enzyme linked immunosorbent assay and conventional serologic methods.
The sensitivity of an enzyme linked immunosorbent assay was compared with 4 other serologic methods for detecting antibodies in B. abortus vaccinated and non-vaccinated cows. Antibodies were detected by ELISA in sera from more than 93% of the culture positive cows, however, less than 55% of the infected animals were identified by any other serologic method. Similarly, antibodies were detected in 82% of the culture negative cows by the ELISA method.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000096:biosynthesis; D002004:Brucella Vaccine / Q000008:administration & dosage* / Q000276:immunology; D002003:Brucella abortus / Q000276:immunology / Q000302:isolation & purification; D002007:Brucellosis, Bovine / Q000276:immunology*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D014611:Vaccination / Q000662:veterinary*
D000907:Antibodies, Bacterial; D002004:Brucella Vaccine