The sucked-flow analyser: a simple apparatus for automatic determinations of steady-state enzyme kinetics.
The sucked-flow analyser is a modified stopped-flow apparatus for automatic measurements of initial rates of enzyme reactions at varying concentrations of substrate or inhibitor. The flow through the system is driven by a water suction pump and regulated by magnetic valves. Sample and reagent solutions are aspirated through tubes whose resistances to laminar flow determine a precise ratio of mixing in the observation cell. A minicomputer controls all operations, collects the data and calculates the results. Measurements on the beta-galactosidase and the cytochrome P-450 reactions are presented.
D000818:Animals; D001322:Autoanalysis; D003201:Computers; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D008722:Methods; D008862:Microsomes, Liver / Q000201:enzymology; D051381:Rats; D001616:beta-Galactosidase / Q000378:metabolism
D004798:Enzymes; D003577:Cytochrome P-450 Enzyme System; D001616:beta-Galactosidase