Stereospecific, reversible binding of D-[3H]glucose to crude membrane fractions prepared from rat brain.
To a crude preparation of synaptic membranes prepared from rat brain, stereospecific, saturable, reversible binding was described of D-[3H]glucose. Binding showed a Kd = 0.45 microM and the fractional rate of dissociation was approximately eight times the fractional rate of association. D-[3H]glucose binding was displaced by 2-deoxyglucose and 3-O-methylglucose and it was abolished when membranes were denatured by heating.
D019325:3-O-Methylglucose; D000818:Animals; D001921:Brain / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008757:Methylglucosides / Q000378:metabolism; D011489:Protein Denaturation; D051381:Rats; D011919:Rats, Inbred Strains; D013570:Synaptic Membranes / Q000378:metabolism*
D008565:Membrane Proteins; D008757:Methylglucosides; D019325:3-O-Methylglucose; D003847:Deoxyglucose; D005947:Glucose