Determination of meta- and para-hydroxyphenylacetic acid levels in single caudate nuclei by selected metastable peak monitoring: a new sensitive gas chromatographic--mass spectrometric procedure.
The levels of meta- and para-hydroxyphenylacetic acids in rat brain caudate nuclei have been determined by a new gas chromatography-mass spectrometric (GC-MS) procedure in which metastable transitions specific to the derivatized acids are observed. The method is more sensitive than previous procedures and allows determination of the acid concentrations using single caudate nuclei. The levels of the meta- and para-isomers were found to be 9.2 +/- 1.5 and 43 +/- 3.7 ng/g, respectively.
D000818:Animals; D002421:Caudate Nucleus / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008991:Monitoring, Physiologic / Q000379:methods*; D010648:Phenylacetates / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D010648:Phenylacetates; C008070:4-hydroxyphenylacetic acid; C008069:3-hydroxybenzeneacetic acid