[Prolonged culture of hepatocytes from healthy adult human livers; immunologic characterization of the hepatocyte nature of cultivated cells].
Monolayer cultures were obtained from human liver explants. Some cells, which extend in all directions, are trypsinized then collected by differential centrifugation and incubated at 38 degrees 5. The oldest culture is 12 months old. Albumin and alpha 1-antitrypsin are constantly detected and measured in the supernatent by radioimmunoassay. The liver specific protein is always characterized by immunofluorescent staining on the surface of the cultivated cells.
D000328:Adult; D002462:Cell Membrane / Q000032:analysis; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D008565:Membrane Proteins / Q000032:analysis; D008856:Microscopy, Fluorescence; D012709:Serum Albumin / Q000378:metabolism; D013997:Time Factors; D000515:alpha 1-Antitrypsin / Q000378:metabolism
D008565:Membrane Proteins; D012709:Serum Albumin; D000515:alpha 1-Antitrypsin
Culture prolongée d'hépatocytes provenant de foies sains de sujets humains adultes; caractérisation immunologique de la nature hépatocytaire des cellules cultivées.