Tetrahydroxylated bile acids in healthy human newborns.
Urine was collected in four healthy infants, born after 35-39 weeks of gestation. The collections were made for 24 h starting immediately after birth. Bile acids were extracted, separated into conjugate groups, solvolysed, hydrolysed and quantified by gas-liquid chromatography as methyl ester trimethylsilyl ether derivatives. Identification was made by gas-liquid chromatography-mass spectrometry. Cholic acid was the predominating primary bile acid. Non-sulphated tetrahydroxylated bile acids, tentatively identified as 1,3,7,12- and 3,6,7,12-tetrahydroxycholanoic acids, were present in almost the same amounts as cholic acid. A previously unknown tetrahydroxylated cholanoic acid, which might be 2-hydroxylated hyocholic acid, was found in all infants in similar amounts. 3 beta-Hydroxy-5-cholenoic acid was present in the sulphate fraction in the urine from all infants. Lithocholic acid was not found. Small amounts of allo and 6-hydroxylated bile acids were mainly found in the sulphate fraction. Bile acids hydroxylated in the 1-position were found predominantly in the taurine fraction.
D000328:Adult; D001647:Bile Acids and Salts / Q000652:urine*; D002757:Cholanes / Q000378:metabolism; D002779:Cholestasis / Q000378:metabolism; D002793:Cholic Acids; D006801:Humans; D006900:Hydroxylation; D007231:Infant, Newborn; D008099:Liver / Q000378:metabolism
D001647:Bile Acids and Salts; D002757:Cholanes; D002793:Cholic Acids; C035925:3,6,7,12-tetrahydroxycholanoic acid; C035924:1,3,7,12-tetrahydroxycholanoic acid; C035926:2,3,6,7-tetrahydroxycholanoic acid