title: Production and characterization of high titre rabbit antigonococcal R-type lipopolysaccharide serum.
abstract: Two methods are reported for the production of rabbit anti-gonococcal lipopolysaccharide sera. One is produced by a conjugate between the core oligosaccharide and bovine serum albumin. The other is obtained by immunizing rabbits with heat-killed, ethanol-acetone washed bacteria. The specificity of the latter serum is studied, and it is shown that most of the antibodies are against a lactose-like determinant situated in the core region of the lipopolysaccharide. Preliminary studies show that this serum is able to cause specific agglutination of gonococci.
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D008070:Lipopolysaccharides / Q000276:immunology*; D009344:Neisseria gonorrhoeae / Q000276:immunology*; D009844:Oligosaccharides / Q000276:immunology; D011817:Rabbits / Q000276:immunology*; D012710:Serum Albumin, Bovine
chemical_list: D007106:Immune Sera; D008070:Lipopolysaccharides; D009844:Oligosaccharides; D012710:Serum Albumin, Bovine