Effects of sulfhydryl reagents on the anti-sickling activity of some membrane-interacting compounds.
Under deoxygenated conditions (PO2 = 0 mmHg), sulfhydryl reagents such as N-ethylmaleimide and iodoacetamide had no effect on sickling, but they did inhibit the anti-sickling activity of chlorpromazine. At the same concentration, these sulfhydryl reagents inhibited the cup formation of chlorpromazine in an oxygenated state (PO2 = 143 mmHg). This supports our previous finding that the anti-sickling effect of membrane-interacting compounds is related to their ability to form a cup-shaped red cell.
D000328:Adult; D000755:Anemia, Sickle Cell / Q000097:blood*; D000986:Antisickling Agents; D002746:Chlorpromazine / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D005033:Ethylmaleimide / Q000494:pharmacology*; D006801:Humans; D007460:Iodoacetamide / Q000494:pharmacology*; D007461:Iodoacetates / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning
D000986:Antisickling Agents; D007461:Iodoacetates; D005033:Ethylmaleimide; D002746:Chlorpromazine; D007460:Iodoacetamide