Mutagenesis and morphological transformation by N-methyl-N'-nitro-N-nitrosoguanidine in the BALB/3T3 clone A31-1-1 cell line.
Concomitant induction of ouabain resistant mutations and morphological transformation in the BALB/3T3 ClA31-1-1-c mouse embryo cell line were obtained after a 30 min treatment with the direct alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Parameters affecting mutation frequencies were determined. The optimal expression time (48 h) for ouabain resistance was independent from the dose of the carcinogen. A linear dose-response relationship for mutation induction was found after treatment with increasing doses of MNNG. The ratio of malignant transformation to mutation frequencies induced by the short treatment with MNNG was found to be within the same order of magnitude over a four-fold dose range. The development of a mutational assay for ouabain resistance in the BALB/3T3 ClA31-1-1-c cell line makes quantitative comparisons possible between mutation and neoplastic transformation frequencies induced by chemical carcinogens in this single cellular system.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002999:Clone Cells; D008769:Methylnitronitrosoguanidine / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D009152:Mutagenicity Tests; D009153:Mutagens; D009154:Mutation
D009153:Mutagens; D008769:Methylnitronitrosoguanidine