Metabolic activation of DMH by colonic microsomes: a process influenced by type of dietary fat.
A study was conducted to determine whether 1,2-dimethylhydrazine (DMH), a potent colon carcinogen, is activated to a more potent mutagen by the drug-metabolizing system of the colonic mucosa and to determine the extent to which this metabolism is modified by lipids in the diet. DMH-treated rats fed a diet enriched with 10% corn oil exhibited markedly elevated colonic enzyme levels for mutagen production. This diet also produced the greatest number of animals with colon tumors, when compared with diets containing other levels and sources of lipid. The potential risk of a diet in which unsaturated fat is the sole source of lipid is underlined.
D019813:1,2-Dimethylhydrazine; D000818:Animals; D001412:Bacillus subtilis / Q000187:drug effects; D001711:Biotransformation; D003106:Colon / Q000201:enzymology / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000209:etiology; D004041:Dietary Fats / Q000008:administration & dosage*; D004127:Dimethylhydrazines / Q000008:administration & dosage / Q000378:metabolism*; D005224:Fats, Unsaturated / Q000009:adverse effects; D008297:Male; D008763:Methylhydrazines / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism; D009092:Mucous Membrane / Q000378:metabolism; D009153:Mutagens / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D004041:Dietary Fats; D004127:Dimethylhydrazines; D005224:Fats, Unsaturated; D008763:Methylhydrazines; D009153:Mutagens; D019813:1,2-Dimethylhydrazine