Primary cultures from fetal rat brain incorporate [3H]-isoleucine and [3H]-valine into immunoprecipitable angiotensin II.
Primary cultures from fetal rat brain contain angiotensin II immunoreactivity in neurons. To study the origin of this immunoreactivity, primary cultures were incubated with [3H]-valine or [3H]-isoleucine. A time-dependent incorporation of radioactivity into immunoprecipitable angiotensin II was observed. Incubation of cultures with Captopril, an inhibitor of the converting enzyme, resulted in a significant inhibition of [3H]-valine incorporation into immunoreactive [3H]-angiotensin II. These results demonstrate that primary cultures from fetal rat brain can synthesize an angiotensin II-like immunoreactivity and support a concept that angiotensin II may be synthesized in fetal rat brain.
D000804:Angiotensin II / Q000096:biosynthesis*; D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D005333:Fetus; D007532:Isoleucine / Q000378:metabolism*; D007700:Kinetics; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium; D014633:Valine / Q000378:metabolism*
D007532:Isoleucine; D014316:Tritium; D000804:Angiotensin II; D014633:Valine