Immunochemical demonstration of a new pregnancy protein in the mare.
An antiserum against the serum of a pregnant mare was absorbed with stallion serum. This antiserum then gave two precipitates in crossed immunoelectrophoresis with serum from pregnant mares as the antigen. The two precipitates exhibited beta-1 and alpha-2 electrophoretic mobility. Identity was demonstrated between the alpha-2 mobile protein and PMSG. The absorbed antiserum inhibited the biological action of the PMSG preparation when tested in mouse ovarian weight assays. The beta-1 mobile protein was not detected in the serum from non-pregnant mares, stallions or geldings and was detected earlier in pregnancy (Day 30) than was PMSG (Day 42).
D000818:Animals; D005260:Female; D006064:Gonadotropins, Equine / Q000276:immunology; D006736:Horses / Q000378:metabolism*; D007123:Immunoelectrophoresis, Two-Dimensional; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood / Q000276:immunology / Q000302:isolation & purification*; D011270:Pregnancy, Animal
D006064:Gonadotropins, Equine; D011257:Pregnancy Proteins