title: Quantitative changes of the alkaloid complex in a submerged culture of Claviceps paspali.
abstract: Claviceps paspali FA produced high concentrations of alkaloid under submerged conditions. Their production was found to depend on the developmental stage and treatment of the filamentous culture inoculum. A medium containing Bacto-peptone with a constant composition of amino acids was selected for the preparation of the inoculum. A two-week fermentation in a synthetic medium with mannitol at 24 +/- 1 degrees C resulted in an increased production of total alkaloids from the original value of 100-200 micrograms/mL to more than 2000 micrograms/mL. Addition of tryptophan did not further increase the production of alkaloids but resulted in changes of the spectrum of some metabolites, 2,3-Dihydroxybenzoic acid accompanied the alkaloids in the fermentation medium. alpha-Hydroxyethyllysergamide was the predominant component of extracellular alkaloids (80% in the first days of fermentation). During fermentation the level of this alkaloid continuously decreased while the concentration of the accompanying alkaloids, i.e. lysergamide and the corresponding minor isomers, increased.
mesh_terms: D000470:Alkaloids / Q000096:biosynthesis*; D002967:Claviceps / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D008237:Lysergic Acid / Q000031:analogs & derivatives / Q000096:biosynthesis; D008353:Mannitol / Q000378:metabolism; D014364:Tryptophan / Q000494:pharmacology
chemical_list: D000470:Alkaloids; D003470:Culture Media; D008353:Mannitol; D014364:Tryptophan; D008237:Lysergic Acid; D005947:Glucose