Evaluation of an enzymic procedure for the measurement of acetaminophen.
We evaluated a new method for measurement of acetaminophen. From acetaminophen, acetate and p-aminophenol are enzymically produced, and the latter product is determined colorimetrically by reaction with o-cresol and ammoniacal copper sulfate. The method is rapid and precise, and results compare well with those by spectrophotometric and liquid-chromatographic techniques. Major metabolites of acetaminophen, some commonly used drugs, or phenolic substances that may be present in patients with liver or renal disease do not interfere.
D000082:Acetaminophen / Q000032:analysis* / Q000097:blood; D000581:Amidohydrolases; D000627:Aminophenols / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D004630:Emergencies; D005069:Evaluation Studies as Topic; D006801:Humans; D007674:Kidney Diseases / Q000097:blood; D008107:Liver Diseases / Q000097:blood; D013053:Spectrophotometry
D000627:Aminophenols; D000082:Acetaminophen; D000581:Amidohydrolases