A rapid DAPI-distamycin method for identification of the mouse Y chromosome.
In a study requiring rapid diagnosis of the chromosomal sex of mice, standard staining methods for mouse chromosomes were evaluated for speed, simplicity, and the quality of staining. None of the techniques previously used for mouse chromosomes were adequate. However, a DAPI-distamycin A method for human karyotyping was successfully adapted for this purpose.
D000818:Animals; D004214:Distamycins; D005456:Fluorescent Dyes; D007211:Indoles; D007621:Karyotyping; D008297:Male; D051379:Mice; D012730:Sex Chromosomes / Q000502:physiology*; D012732:Sex Determination Analysis; D014998:Y Chromosome / Q000502:physiology*
D004214:Distamycins; D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI