Determination of growth kinetics in a renal sarcoma of the rat by double labeling with 14C- and 3H-thymidine.
An in vitro double-labeling technique for determination of kinetic growth parameters was tested; 13 renal sarcomas of rats were incubated after excision under standardized conditions with 14C- and 3H-thymidine successively. Another ten tumors were evaluated after in vivo double injection of 14C and 3H-thymidine into sarcoma-bearing animals. In both groups histoautoradiography was performed using a simple stripping-film method. Results including labeling indices, mitotic indices, length of DNA-synthesis phase and cell-production rate obtained by both methods were in excellent agreement. All values fitted those determined in earlier experiments using a continuous labeling and a fraction-labeled-mitoses method in vivo. Theoretical considerations on the calculation of kinetic parameters by double labeling have been added. The experimental results found in this study represent a base for evaluation of biopsies from human tumors.
D000818:Animals; D001345:Autoradiography; D002250:Carbon Radioisotopes; D002455:Cell Division; D005260:Female; D007680:Kidney Neoplasms / Q000473:pathology*; D008722:Methods; D008940:Mitotic Index; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D012509:Sarcoma / Q000473:pathology*; D012513:Sarcoma, Experimental / Q000473:pathology; D013936:Thymidine / Q000494:pharmacology*; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; D013936:Thymidine