Determination of tamoxifen and metabolites in human serum by high-performance liquid chromatography with post-column fluorescence activation.
Sensitive and reproducible analyses were developed for assaying tamoxifen, monohydroxytamoxifen, N-desmethyltamoxifen, metabolite E [trans-1(4-hydroxyphenyl)1,2-diphenylbut-1-ene] and a new metabolite, metabolite Y [trans-1(4-hydroxyethoxyphenyl)-1,2-diphenylbut-1-ene] in human serum using high-performance liquid chromatography (HPLC). Three different systems were developed for specific purposes. All chromatography was performed using serum extracts made with hexane-butanol. Detection was by fluorimetry of phenanthrene derivatives formed by on-stream UV irradiation with a newly described device for post-column irradiation of the HPLC stream. This device may be of use in other HPLC systems requiring post-column photochemical reactions.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004285:Dogs; D005470:Fluorometry; D006801:Humans; D013629:Tamoxifen / Q000031:analogs & derivatives / Q000097:blood / Q000302:isolation & purification* / Q000378:metabolism
D013629:Tamoxifen; C016601:afimoxifene; C028787:N-desmethyltamoxifen