title: Radioimmunoelectrophoresis, a sensitive method for detecting cleavage of the fifth component of human complement (C5).
abstract: A method has been developed for detecting cleavage of human C5 in serum and whole blood as a consequence of complement activation. Standard, single-dimension immunoelectrophoresis was performed using as antibody a radioiodinated IgG fraction prepared from a commercially available antiserum to human C5. Autoradiographs developed after radioimmunoelectrophoresis of either normal human serum or functionally pure human C5 revealed only one precipitin band. In contrast, when either zymosan-treated serum or trypsin-treated human C5 were examined with this technique, two additional precipitin bands were detected. One migrated more anodally than native C5 while the other remained at the origin (cathode). Radioimmunoelectrophoresis was significantly more sensitive as an indicator of complement activation in human serum than either measurements of total hemolytic complement or a standard assay for complement (C5)-derived chemotactic activity.
mesh_terms: D003167:Complement Activation; D003182:Complement C5 / Q000032:analysis*; D006461:Hemolysis; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D015054:Zymosan / Q000494:pharmacology
chemical_list: D003182:Complement C5; D015054:Zymosan