Lysates of turkey-grown Pasteurella multocida: effects of solubilizing agents on the immunologic properties of membrane vesicles.
Membrane vesicles from lysed suspensions of turkey-grown Pasteurella multocida were treated with various solubilizing agents to release protein that may contain cross-protection factor. Potassium thiocyanate, NaOH-glycine, lithium diiodosalicylate, guanidine hydrochloride, n-butanol, dimethyl sulfoxide, Triton X-100, and sodium lauryl sarcosinate were each tested as solubilizing agents. Vaccines made from combining solubilized membrane vesicles with complete lysate supernatant fluid produced various degrees of protection against challenge exposure with a heterologous serotype of P multocida in turkeys. Only vaccines prepared from membranes that were solubilized with potassium thiocyanate and sodium lauryl sarcosinate protected as well as complete lysate from turkey-grown P multocida. The amount of protein in each vaccine did not relate to protection. Distinct chemical differences were observed between lysates prepared from turkey-grown P multocida and lysates prepared from 41 C broth-grown P multocida. The external morphology of P multocida, after treatment with lysozyme and EDTA, was similar whether grown in broth or in turkeys.
D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D002462:Cell Membrane / Q000187:drug effects / Q000276:immunology; D003470:Culture Media; D005260:Female; D008297:Male; D010325:Pasteurella / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology; D010326:Pasteurella Infections / Q000517:prevention & control / Q000662:veterinary*; D011201:Poultry Diseases / Q000517:prevention & control*; D013501:Surface-Active Agents / Q000494:pharmacology*; D014422:Turkeys / Q000276:immunology* / Q000382:microbiology
D001428:Bacterial Vaccines; D003470:Culture Media; D013501:Surface-Active Agents