Effect of quercetin on human polymorphonuclear leukocyte lysosomal enzyme release and phospholipid metabolism.
Quercetin inhibited in a concentration-dependent manner the release of beta-glucuronidase from human polymorphonuclear leukocytes stimulated with zymosan-activated serum. 3H-arachidonic acid-prelabelled polymorphonuclear leukocytes released 3H-arachidonic acid upon stimulation with zymosan-activated serum and this was associated with a decrease of radioactivity in the phospholipid fraction as determined by thin layer chromatography. Quercetin inhibited the release of 3H-arachidonic acid. These observations suggest that the zymosan-activated serum stimulus activates phospholipase A2 and that phospholipase A2 is inhibited by quercetin. Thus, quercetin alters polymorphonuclear leukocyte phospholipid metabolism and responses to stimulation.
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood; D005419:Flavonoids / Q000494:pharmacology*; D005966:Glucuronidase / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000097:blood; D054467:Phospholipases A2; D010743:Phospholipids / Q000097:blood*; D011794:Quercetin / Q000494:pharmacology*
D001095:Arachidonic Acids; D005419:Flavonoids; D010743:Phospholipids; D016718:Arachidonic Acid; D011794:Quercetin; D010741:Phospholipases A; D054467:Phospholipases A2; D005966:Glucuronidase