title: Effects of tritiated water on germ cells in medaka embryos.
abstract: Embryos of medaka, Oryzias latipes, were exposed to tritiated water and 137Cs gamma rays continuously from the one-cell stage until hatching (10 days at 26 degrees C). Germ cells in the gonads of newly hatched fry were counted in histological sections and compared with controls. The accumulated dose for 50% survival of germ cells was 195 rad for tritium beta rays and 350 rad for 137Cs gamma rays. Female progeny were produced using Yamamoto's method. The 50% survival doses for female germ cells treated in a manner similar to that described above were 140 rad for beta rays and 305 rad for gamma rays. When embryos of medaka were irradiated with gamma rays below an accumulated dose of 475 rad or treated with tritiated water at a concentration of 0.2 mCi/ml or lower, the dose response of the germ cells showed an exponential relationship. It appeared that there was no threshold or significant dose-rate effect for either beta or gamma rays on germ cell survival, and that tritium beta rays were more effective than 137Cs gamma rays in germ cell killing.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002452:Cell Count; D002470:Cell Survival / Q000528:radiation effects; D003470:Culture Media; D004307:Dose-Response Relationship, Radiation; D004781:Environmental Exposure; D005260:Female; D005399:Fishes / Q000196:embryology; D005854:Germ Cells / Q000196:embryology / Q000528:radiation effects*; D008297:Male; D011836:Radiation Tolerance; D012737:Sex Factors; D014316:Tritium
chemical_list: D003470:Culture Media; D014316:Tritium