Generation of angiotensin I by tissues of the human female genital tract.
Tissues from the fetoplacental unit were incubated in medium E 199 at 0 degrees C and following removal of the tissue were incubated at 37 degrees C. Compared to control tissues held at 0 degrees C, large quantities of angiotensin I, measured by means of specific radioimmunoassay, were detected in the media from both amnion and chorion-decidua while only small amounts were found in the media from placenta and umbilical cord. Following prior incubation with antirenin, the generation of angiotensin I was abolished in all tissues. These results indicate that both renin and its substrate are released into the media during incubation at 0 degrees C and suggest the existence of a locally functioning renin-angiotensin system in certain tissues of the human female genital tract.
D000650:Amnion / Q000378:metabolism; D000803:Angiotensin I / Q000032:analysis / Q000378:metabolism*; D000809:Angiotensins / Q000378:metabolism*; D002823:Chorion / Q000378:metabolism; D003470:Culture Media; D003656:Decidua / Q000378:metabolism; D005321:Extraembryonic Membranes / Q000378:metabolism*; D005260:Female; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D014470:Umbilical Cord / Q000378:metabolism*
D000809:Angiotensins; D003470:Culture Media; D000803:Angiotensin I