Distribution and transport of apo- and holocytochrome b5 in the endoplasmic reticulum of rat liver.
The transport and distribution of apo- and holocytochrome b5 was investigated with the aid of specific antibodies. The holoenzyme was found to be localized mainly in the rough and smooth endoplasmic reticulum and in the Golgi system but some precipitation could also be obtained in the outer mitochondrial membranes and in the peroxisomes. The apoenzyme, however, could only be detected in the endoplasmic reticulum-Golgi system, which also was shown to be the sole site for incorporation of the prosthetic heme moiety. Time-course studies revealed that the labeled enzyme appeared both as apoenzyme and as holoenzyme in the rough endoplasmic reticulum 10 min after in vivo injection of radioactive leucine and that further transport to the smooth endoplasmic reticulum occurred within 10 min. The subsequent transport to other organelles, however, required a somewhat longer time and peak radioactivity in outer mitochondrial membranes was not attained until after 40 min.
D000818:Animals; D000918:Antibody Specificity; D001692:Biological Transport; D003573:Cytochrome b Group / Q000276:immunology / Q000378:metabolism*; D015786:Cytochromes b5; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D007425:Intracellular Membranes / Q000201:enzymology; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution; D014357:Trypsin / Q000378:metabolism
D003573:Cytochrome b Group; D015786:Cytochromes b5; D014357:Trypsin