[Subcellular localization of some enzymes in silkworm eggs].
Differential centrifugation of the silkworm (Bombyx mori L.) egg homogenates resulted in nuclear, mitochondrial, lysosomal and cytosol infarctions, which were analyzed for the activities of alanine, aspartate- and tyrosine aminotransferases, 3-glycerophosphate- and lactate dehydrogenases and acid and alkaline DNAases. Alanine- and aspartate aminotransferases as well as 3-glycerophosphate dehydrogenase are localized mainly in the cytosol, where their activities made up to 86.2, 95.4 and 98.4% of their total activity, respectively. The activities of lactate dehydrogenase and acid DNAase are distributed between the nuclear and mitochondrial fractions; this distribution is even in the case of the former enzyme, whereas in the case of the latter the bulk (90.6%) of total enzyme activity is found in the nuclei. In contrast to the other enzymes whose activity is distributed between different cell fractions, tyrosine aminotransferase is localized exclusively in mitochondria, while alkaline DNAase--exclusively in the nuclei. No correlation between the level of enzyme specific activity and its total content in the fractions was established. The role of the enzymes under study in silkworm metabolism is discussed.
D000818:Animals; D012831:Bombyx / Q000201:enzymology*; D002467:Cell Nucleus / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D003851:Deoxyribonucleases / Q000378:metabolism*; D005260:Female; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D010063:Ovum / Q000201:enzymology*; D013347:Subcellular Fractions; D000637:Transaminases / Q000378:metabolism*
D005993:Glycerolphosphate Dehydrogenase; D007770:L-Lactate Dehydrogenase; D000637:Transaminases; D003851:Deoxyribonucleases
Issledovanie subkletochnoĭ lokalizatsii nekotorykh fermentov v iaĭtsakh tutovogo shelkopriada.