Some properties of mycoplasma virus Br 1.
Morphologically mycoplasma virus Br 1 is a typical contractile-tailed bacteriophage with a head 77 nm in diameter and a tail 104 nm long. Its type of nucleic acid was not determined. Br 1 was closely associated with its host cell and assays reflected infectious centres. During growth of Br 1 in mycoplasma cultures at multiplicities of infection (MOI) greater than 0.001, there was a lag period: this was up to 23 hours at an MOI of 35. The mean generation time of a mycoplasma culture infected at MOI up to 235 was 2 hours, compared with 1 hour for an uninfected culture. However in these infected cultures there were viable mycoplasmas all of which appeared to be fully susceptible to Br 1 infection and did not seem to be carrying the virus. Br 1 formed plaques on M. bovirhinis but failed to produce plaques on strains of 8 Mycoplasma, 2 Acholeplasma and 4 bacterial species.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001435:Bacteriophages / Q000187:drug effects / Q000302:isolation & purification / Q000648:ultrastructure; D002725:Chloroform / Q000494:pharmacology; D004986:Ether / Q000494:pharmacology; D008854:Microscopy, Electron; D009174:Mycoplasma; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D013696:Temperature; D010948:Viral Plaque Assay
D000900:Anti-Bacterial Agents; D004986:Ether; D011092:Polyethylene Glycols; D002725:Chloroform; D017830:Octoxynol; C010615:Nonidet P-40