A poly(U)-binding factor stimulating EF-1 activity in the wheat-germ soluble fraction.
A poly(U)-binding activity is present in the high-speed supernatant fraction of embryo homogenates from wheat seeds. The factor responsible for such activity was found to have a stimulatory effect on the elongation factor 1 (EF-1). It copurifies with EF-1L, the lighter form of EF-1, through Sephadex G-200, DEAE-cellulose, hydroxyapatite and poly(U)-Sepharose 4B column chromatography. The two factors could be separated only through a heating step which destroyed EF-1 activity whilst leaving most of the poly(U)-binding activity unaltered.
D001665:Binding Sites; D002352:Carrier Proteins / Q000302:isolation & purification*; D004589:Electrophoresis, Disc; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000378:metabolism*; D011061:Poly A / Q000494:pharmacology; D011072:Poly U / Q000378:metabolism*; D012639:Seeds / Q000032:analysis; D012995:Solubility; D014908:Triticum / Q000032:analysis
D002352:Carrier Proteins; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D011061:Poly A; D011072:Poly U