Free H-Y antigen induces in vitro testicular differentiation of human XX embryonic indifferent gonads.
In accordance with the anchorage site hypothesis, Daudi beta 2-microglobulin (-) HLA (-) human male Burkitt lymphoma cells are incapable of stably maintaining H-Y antigen on their plasma membrane; instead, they excrete it into the culture medium. The proposed testis-organizing function of Daudi H-Y antigen in solution, previously demonstrated in bovine ovarian embryonic cells, has been tested for the first time in human undifferentiated gonads. Cultured in the presence of Daudi excreted H-Y antigen, gonads obtained from early human embryos of 46, XX chromosomal constitution underwent precocious and complete testicular differentiation.
D002454:Cell Differentiation; D046508:Culture Techniques; D005260:Female; D005835:Genitalia / Q000196:embryology*; D006182:H-Y Antigen; D006801:Humans; D008297:Male; D010053:Ovary / Q000196:embryology; D011946:Receptors, Antigen / Q000502:physiology; D013737:Testis / Q000196:embryology; D014960:X Chromosome
D006182:H-Y Antigen; C028507:H-Y antigen receptor; D011946:Receptors, Antigen