gamma Ray induced deoxyribonucleic acid strand breaks. 3' Glycolate termini.
Gamma irradiation of DNA in aqueous solution produces single strand breaks with two types of 3'-termini. These termini have been identified as a simple phosphoryl group and a glycolate moiety attached to the 3'-phosphoryl group via the 2"-OH of glycolic acid. Irradiation of 5'-end labeled poly(dG) produces compounds that comigrate with authentic 3',5'-dGDP and 2'-deoxyguanosine-3'-(phospho-2"-O-glycolic acid-5'-monophosphate by thin layer chromatography and polyacrylamide gel electrophoresis. Irradiation of poly(dGdC) followed by digestion to 3'-nucleotides produces 2'-deoxyguanosine-3'-(phospho-2"-O-glycolic acid). The presence of glycolate termini at some strand breaks may affect intracellular repair of these lesions.
D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000528:radiation effects*; D003848:Deoxyguanine Nucleotides / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005720:Gamma Rays; D006016:Glycolates / Q000032:analysis; D009838:Oligodeoxyribonucleotides / Q000528:radiation effects; D010710:Phosphates / Q000032:analysis
D003848:Deoxyguanine Nucleotides; D006016:Glycolates; D009838:Oligodeoxyribonucleotides; D010710:Phosphates; C030570:oligodeoxyadenylic acid; C037044:2'-deoxyguanosine-3'-(phospho-2''-O-glycolic acid); D004247:DNA; C013193:phosphoglycolate