The interaction of ram proacrosin and acrosin forms with antiserum raised against ram m beta-acrosin.
An antiserum was raised in rabbits to pure m beta-acrosin, the stable form of active acrosin from ram spermatozoa. By use of immunoprecipitation with Protein A-bearing Staphylococcus aureus cells, so as to react antigen with antibody and isolate the complex rapidly, it could be demonstrated that the anti-m beta-acrosin cross-reacted strongly with native proacrosin, with a degenerate proacrosin form, and with at least one form of active acrosin other than m beta-acrosin. Sperm proteins unrelated to acrosin were not recognized. It is concluded that rabbit anti-m beta-acrosin will react specifically with all the major ram acrosin forms; the antiserum can therefore be used with confidence in immunocytochemical studies to locate both proacrosin and acrosin in ram spermatozoa.
D000176:Acrosin / Q000032:analysis / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000276:immunology; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000276:immunology*; D004792:Enzyme Precursors / Q000032:analysis / Q000276:immunology*; D007106:Immune Sera / Q000032:analysis / Q000494:pharmacology; D008297:Male; D008970:Molecular Weight; D011817:Rabbits; D012756:Sheep / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology*
D001323:Autoantibodies; D004792:Enzyme Precursors; D007106:Immune Sera; D010450:Endopeptidases; C022438:proacrosin; D000176:Acrosin