Aging dependent nucleolar and chromatin changes in cultivated fibroblasts.
Adult fibroblasts have already at low population doubling level (PDL) a significant percentage of cells with modified chromatin as compared to embryonic cells where altered chromatin is seen only at high PDL. In fibroblast populations from a patient with Werner's syndrome, the percentage of cells with both altered chromatin and nucleoli was much higher than in the control cultures from normal donors. The data show for the first time nucleoprotein changes in cells from donors with an aging syndrome and reinforce the concept that serial replication of fibroblasts in vitro causes lesions identical to those of aging in vivo.
D000328:Adult; D002460:Cell Line; D002466:Cell Nucleolus / Q000648:ultrastructure*; D002843:Chromatin / Q000648:ultrastructure*; D005260:Female; D005347:Fibroblasts / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron; D008875:Middle Aged; D011247:Pregnancy; D013997:Time Factors
D002843:Chromatin