The SOS chromotest: direct assay of the expression of gene sfiA as a measure of genotoxicity of chemicals.
We used a gene fusion, placing the lacZ gene encoding beta-galactosidase under the control of the sfiA promoter, to construct a new tester strain for genotoxic agents. The assay is performed in a few hours and involves simple enzymatic assays. The dose response curves contain a linear portion which enables to define the SOS Inducing Potency (SOSIP) of compounds. For the compounds tested SOSIPs extend over 7 decades and correlate generally well with the mutagenic potency assayed in the Salmonella/microsome assay (Mutatest) and in a phage induction assay (Inductest). Sensitivities (lowest amount detected) are comparable in the SOS Chromotest and Mutatest but lower in the Inductest. Our results suggest that at least part of the response in the Mutatest depends on the induction of an SOS function, and that most of the genotoxins are inducer of the SOS system -i.e. can lead to activation of the RecA protease.
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000818:Animals; D001711:Biotransformation; D004260:DNA Repair; D005796:Genes; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D009876:Operon; D012486:Salmonella typhimurium / Q000187:drug effects; D001616:beta-Galactosidase / Q000235:genetics
D009153:Mutagens; D015112:4-Nitroquinoline-1-oxide; D001616:beta-Galactosidase