Glucose metabolism of Haemonchus contortus adults: effects of thiabendazole on susceptible versus resistant strain.
Analyses of the in vitro glucose metabolism of Haemonchus contortus adults have confirmed a complex series of end-products: ethanol, n-propanol, propionate, acetate, and CO2 as the major end-products of catabolism. No difference in end-product accumulation was seen between the cambendazole sensitive (BPL) and cambendazole resistant (CR) strains. Thiabendazole (5 mM) in vitro depressed ethanol, propanol, acetate, and propionate accumulation by approximately 42% in the BPL strain. However, in the resistant strain (CR), these end-product accumulations increased by 50% when the worms were exposed to drug in vitro and 80% when exposed in vivo. Resistance manifested by the CR strain appeared to be associated with the ability to increase its carbon flow in the presence of thiabendazole.
D000433:1-Propanol / Q000378:metabolism; D000085:Acetates / Q000378:metabolism; D000693:Anaerobiosis; D000818:Animals; D002159:Cambendazole / Q000494:pharmacology; D002245:Carbon Dioxide / Q000378:metabolism; D004351:Drug Resistance; D000431:Ethanol / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006189:Haemonchus / Q000187:drug effects / Q000378:metabolism*; D011422:Propionates / Q000378:metabolism; D012756:Sheep; D013827:Thiabendazole / Q000494:pharmacology*; D014251:Trichostrongyloidea / Q000378:metabolism*
D000085:Acetates; D011422:Propionates; D002159:Cambendazole; D002245:Carbon Dioxide; D000431:Ethanol; D000433:1-Propanol; D005947:Glucose; D013827:Thiabendazole