Effect of the carbon oxidation level of the energy source on heat of reaction in biosynthesis of a Pseudomonas strain.
The heats of reaction for cell biosynthesis of a strain of Pseudomonas aeruginosa grown on ammonium-nitrogen and fumarate or butyrate as carbon-energy sources have been determined by microcalorimetric experiments, CO2 and molar growth yield determinations. The different oxidation level of the fumarate-carbon (C1+) and butyrate-carbon (C1-) caused a difference on the sign of the heat of reaction for cellular biosynthesis: positive for fumarate (delta H less than 0) and negative for butyrate (delta H greater than 0). It has been possible to state the stoichiometry of the biosynthetic reaction for fumarate only and not for butyrate because of the large pigment production under the experimental conditions adopted: pure oxygen as gas phase at 30 degrees C.
D002087:Butyrates / Q000378:metabolism*; D002151:Calorimetry; D002245:Carbon Dioxide / Q000378:metabolism; D005650:Fumarates / Q000378:metabolism*; D010084:Oxidation-Reduction; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism*; D013816:Thermodynamics
D002087:Butyrates; D005650:Fumarates; D002245:Carbon Dioxide