Induction of humoral immunity and pulmonary mast cells in mice and rats after immunization with aerosolized antigen.
Rats (BN X Wistar) and mice (CBA/Ca) were immunized by exposure in 10-day periods to an aerosol of ovalbumin (OA). In rats this immunization resulted in IgE antibodies detectable at very low levels in bronchial washings, whereas IgG, IgA and IgM antibodies were recorded both in serum and in bronchial washings. In mice, exposure to aerosolized antigen resulted in specific IgE and IgG antibodies in serum. The levels of IgM antibodies were low and no IgA antibodies could be recorded with the enzyme-linked immunosorbent assay (ELISA). Histological examination of lung tissue from immunized rats and mice revealed increased numbers of cells with characteristics of both immature and mature mast cells. In addition, in the rats these cells were more closely located to the bronchi in immunized than in control animals. In the latter animals the mast cells were located around the blood vessels. Immature mast cells were located in the bronchiole-associated lymphatic tissue (BALT) which showed a marked proliferation in immunized animals. The findings indicate that sensitization via the airways provides possibilities to develop a model in rodents for studies of IgE-mediated allergy in the lung.
D000336:Aerosols; D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000008:administration & dosage; D001980:Bronchi / Q000276:immunology; D001982:Bronchial Diseases / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D006099:Granuloma / Q000276:immunology; D007073:Immunoglobulin E / Q000096:biosynthesis; D007136:Immunoglobulins / Q000096:biosynthesis; D008168:Lung / Q000473:pathology; D008297:Male; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D010047:Ovalbumin / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains
D000336:Aerosols; D000941:Antigens; D007136:Immunoglobulins; D007073:Immunoglobulin E; D010047:Ovalbumin