Granuloma pouch assay. IV. Induction of sister-chromatid exchanges in vivo.
SCEs were induced, in vivo, in cells of a rapidly proliferating subcutaneous granulation tissue, initiated by the formation of a subcutaneous air pouch, on the backs of adult male rats (Granuloma Pouch Assay). 2 days after pouch formation the test compounds were applied, and 24 h later the granulation tissue was excised and dissociated into single cells. Isolated cells were cultured in vitro in media containing BrdU, and SCEs were determined within 24-48 h. The spontaneous frequency was 14.4 +/- 1.1 per metaphase. Mitomycin C (MMC) and cyclophosphamide (CP) induced a significant and dose-dependent increase in SCE frequencies. Results obtained after i.v., i.p. and intra-pouch application routes are compared.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002273:Carcinogens / Q000494:pharmacology*; D003434:Crossing Over, Genetic / Q000187:drug effects*; D003520:Cyclophosphamide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006097:Granulation Tissue / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology*; D009153:Mutagens / Q000494:pharmacology; D051381:Rats; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D002273:Carcinogens; D008937:Mitomycins; D009153:Mutagens; D016685:Mitomycin; D003520:Cyclophosphamide