DNA loss in the developing cerebellum of nervous mouse: a flow cytometric study.
Flow-cytometry of cerebellar EGL cells of nervous (nr) mouse revealed a reduction in DNA quantity per cell accompanied by an increase in the DNA dispersion during 6-8 days of postnatal life. This loss of DNA is due to a partial degeneration of the cells of EGL during the first week of postnatal life. The surviving cells later on achieve a DNA content equivalent to their bone marrow cells. The flow-cytometric technique also facilitates an early detection of the homozygous nervous mutant, almost 2 weeks before the clinical manifestation of the disease, which to date was not possible.
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D002525:Cerebellar Cortex / Q000254:growth & development / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D005434:Flow Cytometry; D006720:Homozygote; D051379:Mice; D008809:Mice, Inbred C3H; D008818:Mice, Neurologic Mutants / Q000254:growth & development / Q000378:metabolism*
D004247:DNA