Demonstration of gene dosage effects on antigens in the Duffy, Ss, and Rh systems using an enzyme-linked immunosorbent assay.
Using a recently developed enzyme-linked immunosorbent assay, gene dosage effects were demonstrated for the Duffy, Ss, and Rh red blood cell antigen systems. We report a comparison by an antibody-binding assay of the relative amounts of Fya and Fyb antigens on FyaFya, FyaFyb, FybFyb, FyaFyx, and FyxFyx erthrocytes, and of s antigen on cells homozygous and heterozygous for s. The immunosorbent assay also was used to study Rh antigens, and data which had been obtained using radiolabelled antibodies were confirmed.
D001666:Binding Sites, Antibody; D001789:Blood Group Antigens / Q000235:genetics*; D004375:Duffy Blood-Group System / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000276:immunology; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007124:Immunoenzyme Techniques; D012204:Rh-Hr Blood-Group System / Q000235:genetics
D001789:Blood Group Antigens; D004375:Duffy Blood-Group System; D012204:Rh-Hr Blood-Group System