A method for rapid, sensitive quantitation of short-patch DNA repair in cultured rat hepatocytes.
The sensitivity of DNA-repair detection after incubation of cultured rat hepatocytes with dimethylnitrosamine (DMN) and [Me-1',2'-3H]thymidine ([3H]TdR) was enhanced by the preparation of isolated nuclei. Nuclear isolation-liquid scintillation counting (NI-LSC) provided a rapid method for the determination of unscheduled DNA synthesis (UDS). DMN-induced UDS detected by autoradiography and NI-LSC correlated well and provided similar dose-response curves, indicating the utility of the NI-LSC method for the quantitation of short-patch DNA repair.
D000818:Animals; D001711:Biotransformation; D002478:Cells, Cultured; D004260:DNA Repair; D004128:Dimethylnitrosamine / Q000494:pharmacology; D008099:Liver / Q000166:cytology; D051381:Rats; D013936:Thymidine / Q000378:metabolism
D004128:Dimethylnitrosamine; D013936:Thymidine