[Cell survival of the Brucella abortus strain 19 vaccine during the lyophilization process according to the vehicle used].
Ten stabilizing media commonly used by commercial laboratories for the stabilization of Brucella abortus strain 19 for lyophilization were studied. Six media gave similar results, with viable count averages ranging from 50% to 58%. The standard stabilizer recommended by the World Health Organization (casitone 2,5%, sucrose 5% and sodium glutamate 1%) was included. The other four media gave viabilities with averages from 19,7% to 38,7%, which represented a highly significant difference. The lowest viable count was observed with the 5% polyvinylpyrrolidone stabilizer. The differences in the number of viable cells among the various processes were greater than expected.
D002004:Brucella Vaccine / Q000592:standards*; D002003:Brucella abortus / Q000254:growth & development* / Q000276:immunology; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D005612:Freeze Drying; D014677:Pharmaceutical Vehicles / Q000494:pharmacology*; D011205:Povidone / Q000494:pharmacology
D002004:Brucella Vaccine; D014677:Pharmaceutical Vehicles; D011205:Povidone
Supervivencia de las células de la vacuna Brucella abortus cepa 19 durante el proceso de liofilización según el vehículo utilizado.