Measurement of rat brown-adipose-tissue mitochondrial uncoupling protein by radioimmunoassay: increased concentration after cold acclimation.
A specific antiserum has been raised against the 32 000-mol.wt. uncoupling protein from the mitochondria of rat brown adipose tissue and a sensitive radioimmunoassay for the protein has been developed. The uncoupling protein is present in large amounts in brown adipose tissue; its concentration is increased substantially by cold acclimation. The protein has not been detected in the liver, heart, or parametrial white adipose tissue of rats.
D000222:Adaptation, Physiological; D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D000918:Antibody Specificity; D003080:Cold Temperature; D005260:Female; D008928:Mitochondria / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D014018:Tissue Distribution; D014475:Uncoupling Agents / Q000032:analysis*
D011506:Proteins; D014475:Uncoupling Agents