Dihydropyridines as potent calcium channel blockers in neuronal cells.
Nicardipine, one of the dihydropyridine derivatives, in a nanomolar concentration range suppressed the high K+ -induced neurotransmitter release from cultured neuronal cells (chick embryonic neural retina cells and clonal rat pheochromocytoma cells). The high K+ -induced Ca2+ uptake into pheochromocytoma cell was also blocked by nicardipine in the same concentration range. [3H]Nitrendipine, another dihydropyridine derivative, bound specifically to pheochromocytoma cell homogenate in a saturable manner. We concluded that dihydropyridines block and bind to the high K+ -sensitive Ca2+ channels in neuronal cells.
D000310:Adrenal Gland Neoplasms; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002122:Calcium Chloride / Q000494:pharmacology; D002642:Chick Embryo; D002999:Clone Cells; D009374:Neoplasms, Experimental; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D009529:Nicardipine; D009543:Nifedipine / Q000031:analogs & derivatives / Q000494:pharmacology; D009638:Norepinephrine / Q000378:metabolism; D010673:Pheochromocytoma; D011188:Potassium / Q000502:physiology; D011725:Pyridines / Q000494:pharmacology*; D051381:Rats; D012160:Retina
D002121:Calcium Channel Blockers; D011725:Pyridines; D009529:Nicardipine; D009543:Nifedipine; D002122:Calcium Chloride; D011188:Potassium; D002118:Calcium; D009638:Norepinephrine