[Autoradiographic detection of 3H-labelled glycoproteins in "light" and "dark" neurons of gray matter of the rat brain following intraventricular application of 3H-fucose. Preliminary report].
The simultaneous demonstration of 3H-radioactivity and "light" and "dark" neurons by histological staining revealed that the occurrence of "light" and "dark" neurons are morphological correlates of different activity stages of cell metabolism. In this connection it became evident that "light" nerve cells incorporated significantly greater amounts of fucose and therefore should be regarded as metabolically more active. We also present evidence that "dark" neurons are not artificial products caused by postmortem trauma to the brain inadequately fixed (as pointed out by Cammermeyer).
D000818:Animals; D001345:Autoradiography; D001923:Brain Chemistry; D002552:Cerebral Ventricles / Q000032:analysis* / Q000166:cytology / Q000378:metabolism; D005643:Fucose / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006651:Histocytochemistry; D008297:Male; D009474:Neurons / Q000032:analysis / Q000145:classification* / Q000378:metabolism; D010487:Periaqueductal Gray / Q000032:analysis / Q000166:cytology / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D006023:Glycoproteins; D005643:Fucose
Autoradiographischer Nachweis von 3H-markierten Glykoproteinen in "light" und "dark" Neuronen verschiedener Grisea des Rattenhirnes nach intraventrikulärer 3H-Fucose-Applikation. Vorläufige Mitteilung.