title: The role of UVB radiation in the induction and elicitation of photocontact hypersensitivity to TCSA in the mouse.
abstract: Photocontact hypersensitivity (PHS) to 3,3',4',5 tetrachlorosalicylanilide (TCSA) can be induced in mice by using cyclophosphamide as an immunopotentiator. Only UVA (320-400 nm) radiation was required for both sensitization and elicitation of PHS. The reaction was successfully transferred to syngeneic mice by injecting them with lymph node cells from sensitized donors, a finding that demonstrates the immunologic nature of PHS. The presence of UVB (280-320 nm) radiation was not necessary for sensitization and did not increase PHS beyond the levels observed with UVA radiation alone. Ultraviolet radiation in the UVB range (plus a small amount of UVA radiation) from FS40 sunlamps in the dose employed did not induce statistically significant PHS to TCSA, nor did it elicit a significant response in mice sensitized with TCSA plus UVA radiation. However, treatment of mice with UVB radiation at a distant site 6 days before sensitization suppressed the induction of PHS. This suppression appeared to be analogous to the systemic suppression of ordinary contact hypersensitivity in mice by UVB radiation.
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D005260:Female; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D010787:Photosensitivity Disorders / Q000209:etiology / Q000276:immunology*; D011833:Radiation Injuries, Experimental; D012457:Salicylamides / Q000276:immunology*; D012458:Salicylanilides / Q000276:immunology*; D014466:Ultraviolet Rays
chemical_list: D000276:Adjuvants, Immunologic; D012457:Salicylamides; D012458:Salicylanilides; D003520:Cyclophosphamide; C003390:3,3',4',5-tetrachlorosalicylanilide