Murine immunosuppression with mycoviral dsRNA.
The effect of three different size molecular weight species of mycoviral dsRNA on the immune response to sRBC was tested in C57Bl/6 mice. The various dsRNA species were extracted from electrophoresis polyacrylamide-agarose slab gels. Their molecular weights ranged from 1.0 x 10(6) daltons to 3.5 x 10(6) daltons. All three sizes of mycoviral dsRNA significantly (p less than 0.0001) suppressed the hemolytic antibody titer of mice 8 days after immunizations with 15 micrograms dsRNA/mouse and 10(8) sRBC when compared to control mice which received only sRBC. No immune suppression was observed in any of the mice challenged with a second sRBC immunization 60 days after the first inoculations. Hemagglutination titers at this time were typical of a secondary antibody response to sRBC. In conclusion these three molecular weight mycoviral dsRNA species appeared to be potent immunosuppressors when approximately 15 micrograms/mouse were used.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation; D005260:Female; D006386:Hemagglutination Tests; D007108:Immune Tolerance; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D010408:Penicillium chrysogenum / Q000276:immunology; D012367:RNA, Viral / Q000276:immunology*; D012441:Saccharomyces cerevisiae / Q000276:immunology
D000276:Adjuvants, Immunologic; D012367:RNA, Viral