N-Trimethylalanine, a novel blocked N-terminal residue of Tetrahymena histone H2B.
Our previous study showed that the amino acid sequence of Tetrahymena histone H2B has a blocked N-terminal residue followed by 119 amino acid residues, X-Pro-Lys.... [J. Biochem. (1982) 91, 897-904]. Now, X has been found to be N-trimethylalanine by 1H nuclear magnetic resonance spectroscopy of the isolated X-Pro-Lys and X. This identification was supported by mass spectrometry of X-Pro-Lys and by column and paper chromatography of X. This is the first time that N-terminal blocking by methylation has ever been found in histones.
D000409:Alanine / Q000031:analogs & derivatives* / Q000032:analysis; D000818:Animals; D006657:Histones / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D013769:Tetrahymena pyriformis / Q000032:analysis*
D006657:Histones; C015561:trimethylalanine; D000409:Alanine