The kinetic mechanism of the major form of ox kidney aldehyde reductase with D-glucuronic acid.
The steady-state kinetics of the major form of ox kidney aldehyde reductase with d-glucuronic acid have been determined at pH7. Initial rate and product inhibition studies performed in both directions are consistent with a Di-Iso Ordered Bi Bi mechanism. The mechanism of inhibition by sodium valproate and benzoic acid is shown to involve flux through an alternative pathway.
D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D005965:Glucuronates / Q000378:metabolism*; D020723:Glucuronic Acid; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008956:Models, Chemical; D009249:NADP / Q000378:metabolism; D013400:Sugar Acids / Q000378:metabolism; D014635:Valproic Acid / Q000494:pharmacology
D005965:Glucuronates; D007527:Isoenzymes; D013400:Sugar Acids; C015252:gulonic acid; D009249:NADP; D014635:Valproic Acid; D020723:Glucuronic Acid; D000429:Alcohol Oxidoreductases; C019642:alcohol dehydrogenase (NADP+)