title: The effect of cycloheximide on dictyosome activity in Tradescantia pollen tubes determined using cytochalasin D.
abstract: Dictyosome activity in Tradescantia pollen tubes has been determined using a recently developed method based on the assumption that the rate of vesicle accumulation around the dictyosomes, after treatment with cytochalasin D, is equivalent to the actual rate of vesicle production. In tubes germinated in the presence of 1.0 micrograms/ml cycloheximide, reduced dictyosome activity could be detected as early as 10 min after sowing, although tube extension was not halted until later. After 30 min vesicle production had completely ceased. These observations are discussed in relation to previous reports on the effect of cycloheximide on pollen tube growth, and in relation to the synthesis and transfer of membrane proteins to secretory vesicles and the plasma membrane. It is concluded that the ability of pollen to germinate and produce short tubes in the presence of cycloheximide, does not necessarily indicate that protein synthesis is not a requirement for early pollen tube growth, as protein shortages would not be expected to become apparent over time periods less than the dictyosome turnover time and the secretory vesicle residence time.
mesh_terms: D003513:Cycloheximide / Q000494:pharmacology*; D015638:Cytochalasin D; D003572:Cytochalasins / Q000494:pharmacology*; D008854:Microscopy, Electron; D009940:Organoids / Q000187:drug effects / Q000648:ultrastructure; D010944:Plants / Q000187:drug effects* / Q000648:ultrastructure; D011058:Pollen
chemical_list: D003572:Cytochalasins; D015638:Cytochalasin D; D003513:Cycloheximide