title: Quantitation of radiolabeled compounds eluting from the HPLC system.
abstract: Three techniques are compared for the quantitation of various radiolabeled compounds eluting in the high performance liquid chromatography system. The first technique requires fraction-collecting the effluent from the HPLC, removing an aliquot to scintillation vials, and counting each fraction in a liquid scintillation counter. The second uses direct interface of the HPLC effluent to a flow-through radioactivity detector. The third involves quantitation of various radiolabeled compounds (proteins, steroids, and nucleotides) by splitting the effluent from the HPLC with an electronic steam splitter, thus diverting a present portion to the fraction collector for further chemical characterization and the remainder to the radioactivity flow detector for direct quantitation. A direct comparison of the chromatograms and the radioactivity counting efficiencies of these three techniques is presented.
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000882:Haplorhini; D006801:Humans; D008297:Male; D009711:Nucleotides / Q000378:metabolism; D011284:Pregnenolone / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D011868:Radioisotopes / Q000032:analysis*; D012708:Sertoli Cells / Q000378:metabolism; D013737:Testis / Q000032:analysis; D013739:Testosterone / Q000378:metabolism
chemical_list: D009711:Nucleotides; D011506:Proteins; D011868:Radioisotopes; D013739:Testosterone; D011284:Pregnenolone