A simple technique using skin implants to produce histocompatability (BoLA) typing sera.
We describe a technique to produce high-titered bovine lymphocytotoxic antisera using skin implants. The main advantage of this technique is that the skin does not need to be processed prior to implantation and no surgical skill is required. In addition, the skin can be stored for up to 2 weeks and can be shipped to other laboratories without special handling and without loss of immunogenicity.
D000818:Animals; D000917:Antibody Formation; D000961:Antilymphocyte Serum; D002417:Cattle / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006649:Histocompatibility Antigens / Q000276:immunology*; D006650:Histocompatibility Testing; D008214:Lymphocytes / Q000276:immunology*; D016038:Skin Transplantation
D000961:Antilymphocyte Serum; D006649:Histocompatibility Antigens