The measurement of human erythrocyte carbonic anhydrase I by the enzyme linked immunosorbent assay (ELISA).
A method is described for the assay of human erythrocyte (RBC) carbonic anhydrase I (HCAI) by the enzyme linked immunosorbent assay. The method was found to be a simple, reliable and precise technique and gave a mean C.V. for 40 samples, assayed in quadruplicate, of 3.32% and a range of 0.84-5.9. The mean erythrocyte HCAI value and standard deviation for 20 normal men and women were respectively 16.9 +/- 3.4 and 15.4 +/- 2.1 mg HCAI/g haemoglobin. The use of heparin as an anticoagulant interfered with the assay resulting in apparent HCAI concentrations as low as 60% of those obtained using EDTA.
D000818:Animals; D002256:Carbonic Anhydrases / Q000097:blood*; D055598:Chemical Phenomena; D002621:Chemistry; D004492:Edetic Acid; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D006493:Heparin; D006801:Humans; D008297:Male; D011817:Rabbits; D012016:Reference Values; D012737:Sex Factors
D006493:Heparin; D004492:Edetic Acid; D002256:Carbonic Anhydrases