[1st immunocytochemical application of an anti-dopamine antibody in the study of the central nervous system].
An antibody against dopamine was raised in Rabbits by means of an immunogen constituting dopamine coupled to bovine serum albumin by formaldehyde. These antibodies were applied to frozen sections of Goldfish (Carassius auratus) brain fixed by 4% paraformaldehyde. The localisation of immunoreactive structures corresponded to regions rich in dopamine detected by histofluorescence. The specificity of this antiserum tested with the aid of dopamine analogues coupled with a carrying protein different from that of the antibody, indicates its validity as a method for the detection of dopaminergic neurons.
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001921:Brain / Q000166:cytology; D001923:Brain Chemistry; D003530:Cyprinidae / Q000378:metabolism*; D004298:Dopamine / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006054:Goldfish / Q000033:anatomy & histology / Q000378:metabolism*; D006651:Histocytochemistry; D009474:Neurons / Q000032:analysis; D011817:Rabbits
D000906:Antibodies; D004298:Dopamine
Première application immunocytochimique d'un anticorps antidopamine à l'étude du système nerveux central.