Enhancement of sulfanilamide N4-hydroxylase activity in kidney and liver microsomes of rats by pretreatment with 3-methylcholanthrene type-polychlorinated biphenyl.
N4-Hydroxylation of sulfanilamide was studied with kidney and liver microsomes of rats by means of high performance liquid chromatography. In kidney microsomes, both contents of cytochrome P-450 (448) and activity of N4-hydroxylase were markedly increased by pretreatment with 3,4,5,3',4'-pentachlorobiphenyl (PenCB) (about 4 times), although neither one was increased by 3-methylcholanthrene (3-MC) and phenobarbital (PB) pretreatments. In liver microsomes, on the other hand, both N4-hydroxylase activity and cytochrome P-450 (448) contents were increased by either PenCB or 3-MC pretreatment, whereas by PB pretreatment, N4-hydroxylase activity was not changed although cytochrome P-450 contents was increased 2-fold.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D004790:Enzyme Induction; D006863:Hydrogen-Ion Concentration; D006900:Hydroxylation; D066298:In Vitro Techniques; D007668:Kidney / Q000201:enzymology*; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology*; D008861:Microsomes / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013424:Sulfanilamides / Q000378:metabolism*; D000096926:Benzenesulfonamides
D003577:Cytochrome P-450 Enzyme System; D008748:Methylcholanthrene; D006899:Mixed Function Oxygenases; D010634:Phenobarbital; D011078:Polychlorinated Biphenyls; D013424:Sulfanilamides; C005533:4-hydroxylaminobenzenesulfonamide; D000096926:Benzenesulfonamides; C037343:sulfanilamide N-hydroxylase; C023035:3,4,5,3',4'-pentachlorobiphenyl