Molecular cloning and analysis of the endogenous retrovirus chemically induced from RFM/Un mouse cell cultures.
We molecularly cloned and analyzed an N-tropic ecotropic retrovirus induced with iododeoxyuridine from RFM/Un mouse cell cultures. Based on the restriction map, the RFM/Un virus appears to be indistinguishable from other induced N-tropic retroviruses. A nucleotide sequence analysis of the long terminal repeat of an infectious clone revealed structural features characteristic of murine type C retrovirus long terminal repeats. The U3 region of the RFM/Un virus long terminal repeat, however, contained no short sequence duplication or insertion found in other murine leukemia virus isolates.
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis; D005347:Fibroblasts; D007065:Idoxuridine / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D012190:Retroviridae / Q000254:growth & development*; D014775:Virus Activation / Q000187:drug effects*
D004279:DNA, Viral; D007065:Idoxuridine