The synthesis and transport of fucosylated glycans in the immature mouse cerebellum. An autoradiographic and microchemical study of differentiating cell and tissue compartments.
A study of 3H-fucose incorporation in the immature mouse parenchyma revealed that the rate of synthesis of fucosylated glycans in the neuropil of more differentiated internal granular layer, as well as in the PURKINJE cell bodies is higher than in the less mature external granular layer. The 3H-fucose incorporation into the choroid plexus, meninges, and blood vessel walls exceeds highly incorporation into the cerebellar parenchyma. A follow-up autoradiographic study revealed remarkable differences in the radioactivity of incorporated 3H-fucose in the "cell-body rich" and "cell-fibre rich" layers indicating transport of newly synthesized glycans from the perikarya of differentiating cells into their processes extending mainly into the molecular layer and the cerebellar medulla. It is assumed that the increased rate of 3H-fucose incorporation reflect 1. increasing complexity of the fucosylated glycans of differentiating cells 2. increase in the turnover of cell membrane components, 3. accelerated export of glycosylated compounds into the outgrowing fibres of differentiating cerebellar cells.
D000818:Animals; D001345:Autoradiography; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism* / Q000648:ultrastructure; D005643:Fucose / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D011134:Polysaccharides / Q000096:biosynthesis* / Q000378:metabolism
D011134:Polysaccharides; D005643:Fucose