Rapid immunochemical determination of plasma fibronectin with a centrifugal analyzer.
We report a turbidimetric procedure, using commercially available reagents, for the determination of plasma fibronectin with an unmodified centrifugal analyser. Fixed-time kinetic readings at 340 nm were taken at 60 and 720 s. A wide range of concentration (up to 1000 mg/l) can be measured. Precision and accuracy were quite acceptable. Correlation with radial immunodiffusion was satisfactory. This automated procedure, simple and rapid, allows extensive studies of fibronectin in various diseases.
D001322:Autoanalysis / Q000379:methods*; D004355:Drug Stability; D005353:Fibronectins / Q000097:blood*; D006801:Humans; D007118:Immunoassay; D005779:Immunodiffusion; D007700:Kinetics; D009391:Nephelometry and Turbidimetry; D012016:Reference Values
D005353:Fibronectins