title: Different tissue concentrations and rates of synthesis of dolichol, dolichyl acyl esters, and dolichyl phosphate in mouse testes and preputial glands.
abstract: The rates of synthesis and tissue concentrations of dolichol derivatives differ markedly in testes and preputial glands from adult C57BL/6J mice. In testes, the rates of free dolichol, dolichyl acyl esters, and dolichyl phosphate synthesis were approximately 5,000, 4,000 and 2,000 dpm/3h/g, respectively. Comparable rates for preputial glands were 12,000, 60,000 and 0 dpm/3h/g. Thus in testes, dolichyl phosphate represented 15-20% of total dolichol synthesis, whereas no de novo dolichyl phosphate synthesis was detected in preputial glands. In testes, free dolichol was the predominant derivative synthesized; in preputial glands, 80% of dolichol synthesized was esterified to fatty acids. The concentration of total dolichol (free alcohol plus acyl esters) was 160 micrograms/g tissue in testes and 1270 micrograms/g tissue in preputial glands. In both tissues, 85-90% of dolichol was esterified to fatty acids, and no dolichyl phosphate was detected.
mesh_terms: D000818:Animals; D004224:Diterpenes / Q000378:metabolism*; D004288:Dolichol Phosphates / Q000096:biosynthesis / Q000378:metabolism*; D004286:Dolichols / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000378:metabolism*; D005088:Exocrine Glands / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011106:Polyisoprenyl Phosphates / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*
chemical_list: D004224:Diterpenes; D004288:Dolichol Phosphates; D004286:Dolichols; D011106:Polyisoprenyl Phosphates; C028306:dolichol monophosphate