In vitro block of murine L 1210 leukemia cell growth by amiloride, an inhibitor of passive Na+ influx.
The present study was aimed to decide whether Na+ influx can be involved in regulation of murine L 1210 leukemia cell growth. Cells were cultivated in the presence of different concentrations of amiloride and cellular growth was monitored by 3H-thymidine incorporation/10(5) cells. This drug inhibited cell growth in concentrations ranging from 1 X 10(-5) to 1 X 10(-3) mmol/ml. Even short time treatments with amiloride caused irreversible alterations: the cells, although survived, lost their ability to divide. The results support the hypothesis that Na+ influx is necessary for the duplication of tumor cells.
D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007939:Leukemia L1210 / Q000378:metabolism*; D008297:Male; D051379:Mice; D011719:Pyrazines / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism*
D011719:Pyrazines; D000584:Amiloride; D012964:Sodium