Search for ligands of neuronal alpha-bungarotoxin receptors.
Extracts of calf brain were analyzed for substances capable of blocking the binding of [125I]-alpha-bungarotoxin to chick brain membrane preparations, and shown to contain blocking activity that was insensitive to heating and trypsin. Fractionation on Sephadex G-25 yielded two components, one representing nonspecific inhibition by inorganic cations, the other identified as choline by co-chromatography experiments and analysis of the purified inhibitor using thin layer chromatography and mass spectrometry. These results support the notion that the alpha-bungarotoxin binding macromolecule in the central nervous system is an acetylcholine receptor.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002038:Bungarotoxins / Q000378:metabolism; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006358:Hot Temperature; D009474:Neurons / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000378:metabolism*; D011978:Receptors, Nicotinic; D014357:Trypsin / Q000378:metabolism; D064569:alpha7 Nicotinic Acetylcholine Receptor
D002038:Bungarotoxins; D011950:Receptors, Cholinergic; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor; D014357:Trypsin