Ro 22-5417, a new clavam antibiotic from Streptomyces clavuligerus. II. Fermentation, isolation and structure.
The purification of a new antibiotic, Ro 22-5417, was achieved by a variety of preparative column chromatographic methods. The antibiotic was obtained crystalline with an overall recovery of 3% after a 300-fold purification. The structure was determined from proton and 13C NMR spectra to be 3-(7-oxo-1-aza-4-oxabicyclo[3.2.0]hept-3-yl)alanine.
D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography / Q000379:methods; D002969:Clavulanic Acids / Q000302:isolation & purification*; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D013055:Spectrophotometry, Infrared; D013302:Streptomyces / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D002969:Clavulanic Acids; C037743:3-(7-oxo-1-aza-4-oxabicyclo(3.2.0)hept-3-yl)alanine