The effect of heparin on the porcine lymphocyte chromatin--I. The comparative study of DNA in different chromatin fractions.
1. Porcine lymphocyte chromatin in the solution of 0.15 M NaCl + 0.01 M Tris, pH 7 treated with heparin liberated 30% protein and 7.5% DNA to the supernatant. 2. DNA from the supernatant and the pellet fractions as well as from control chromatin were isolated in identical conditions. 3. No significant changes were observed in spectral properties and melting points in SSC of comparable DNA specimens. 4. It was noted, however, that DNA of the supernatant is subject to denaturation in the process of isolation, which, apart from the difference in protein composition of the supernatant and the pellet fractions, suggests different chromatin structure of these fractions.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000302:isolation & purification*; D006493:Heparin / Q000494:pharmacology*; D008214:Lymphocytes / Q000378:metabolism*; D009691:Nucleic Acid Denaturation; D013552:Swine
D002843:Chromatin; D006493:Heparin; D004247:DNA