title: Synthesis of the C-terminal half of thymosin alpha 1 by the polymeric reagent method.
abstract: In this report we further show the utility and efficiency of polymer-bound 1-hydroxybenzotriazole (PHBT) as an almost ideal support for the polymeric reagent method of peptide synthesis. This was demonstrated by the synthesis of thymosin alpha 1 (15-28), in which two suitably blocked segments, Boc-Asp (OtBu)-Leu-Lys (2Cz)-Glu (OBzl)-Lys (2Cz)-Lys (2Cz)-OH (3) and Boc-Glu (OBzl)-Val-Val-Glu (OBzl)-Glu (OBzl)-Ala-Glu (OBzl)-Asn-OBzl (2), were prepared entirely by utilizing PHBT activation for each coupling step. After appropriate deblocking of 2, segments 2 and 3 were coupled by the DCC-HOBT method, followed by complete deblocking and ion-exchange chromatographic purification, affording the C-terminal half of thymosin alpha 1, H-Asp-Leu-Lys-Glu-Lys-Lys-Glu-Val-Val-Glu-Glu-Ala-Glu-Asn-OH (1).
mesh_terms: D002852:Chromatography, Ion Exchange; D007202:Indicators and Reagents; D009904:Optical Rotation; D010446:Peptide Fragments / Q000138:chemical synthesis; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives / Q000138:chemical synthesis*; D013951:Thymus Hormones / Q000138:chemical synthesis*
chemical_list: D007202:Indicators and Reagents; D010446:Peptide Fragments; D013951:Thymus Hormones; D013947:Thymosin; D000077596:Thymalfasin