Induction of endoreduplication in Chinese hamsters V79 cells by cytosine arabinoside.
Endoreduplication (ER) could be induced very effectively in Chinese hamster V79 cells exposed to cytosine arabinoside (1-beta-D-arabinofuranosylcytosine; Ara-C). Cells were cultured for 48 hours in Ara-C containing medium. ER frequency increases rapidly after Ara-C release. About 60% of metaphase cells were endoreduplicated at 8-10 hours after release from Ara-C (5 micrograms/ml). Induction of ER also depends on Ara-C concentrations.
D000818:Animals; D002460:Cell Line; D002875:Chromosomes / Q000187:drug effects*; D003078:Colchicine / Q000494:pharmacology; D006224:Cricetinae; D003412:Cricetulus; D003561:Cytarabine / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D013997:Time Factors
D003561:Cytarabine; D004247:DNA; D003078:Colchicine