Simultaneous determination of tryptophan and its metabolites in mouse brain by high-performance liquid chromatography with fluorometric detection.
A new method for the determination of tryptophan and its metabolites in a single mouse brain using high-performance liquid chromatography (HPLC) with fluorometric detection is described. Tryptophan, serotonin, 5-hydroxyindoleacetic acid, indoleacetic acid, and tryptophol were clearly separated by a C8 reverse-phase column. Tissue preparation is performed only to centrifuge homogenates of brain prior to the injection to HPLC. The sensitivity is in the range from 10 to 15 pg.
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D008297:Male; D051379:Mice; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000032:analysis*
D014364:Tryptophan