Singlet oxygen production by lactoperoxidase.
Infrared chemiluminescence with maximum intensity near 1270 nm was detected in the lactoperoxidase, H2O2, Br- system. No emission was observed when heat-inactivated enzyme was used or when Br- or H2O2 was deleted from the system. The chemiluminescence was increased by a factor of 42 +/- 5 (mean +/- S.E.) in 98% 2H2O. These results are strong evidence for the production of 1O2 in this system.
D001965:Bromides; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism; D007784:Lactoperoxidase / Q000378:metabolism*; D008163:Luminescent Measurements; D010100:Oxygen / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D013055:Spectrophotometry, Infrared
D001965:Bromides; D006861:Hydrogen Peroxide; D007784:Lactoperoxidase; D010544:Peroxidases; D010100:Oxygen