Fluorimetric determination of carbamoyl phosphate.
A simple fluorimetric assay for the determination of carbamoyl phosphate in tissue extracts is described. In the assay, production of ATP from carbamoyl phosphate and ADP by carbamate kinase is coupled to the formation of NADPH, using glucose, hexokinase, NADP+, and glucose-6-phosphate dehydrogenase. Production of NADPH in this system proved to be equal to the amount of carbamoyl phosphate present.
D000244:Adenosine Diphosphate / Q000302:isolation & purification; D000255:Adenosine Triphosphate / Q000302:isolation & purification; D000818:Animals; D002219:Carbamates / Q000032:analysis*; D002221:Carbamyl Phosphate / Q000032:analysis*; D008297:Male; D008930:Mitochondria, Liver / Q000032:analysis; D009249:NADP / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence
D002219:Carbamates; D009249:NADP; D002221:Carbamyl Phosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate