Rapid visualization of proteins in isoelectric focusing gels: isolation of brain tubulin subspecies.
A method for visualization of unmodified proteins in polyacrylamide isoelectric focusing (IEF) gels is described. The proteins appear as white, translucent bands when disc IEF gels are placed in water. The gel can be scanned with a scanning spectrophotometer, or the protein bands can be excised using a simple apparatus, which is described, and the protein eluted. The method is fast, selective for proteins, sensitive, and quantitative. It has been used to isolate tubulin species separated by as little as 0.5 mm in disc IEF gels.
D000818:Animals; D001923:Brain Chemistry; D007525:Isoelectric Focusing / Q000295:instrumentation / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D014404:Tubulin / Q000302:isolation & purification*
D014404:Tubulin