title: Isolation of laminin from human placental basement membranes: amnion, chorion and chorionic microvessels.
abstract: Laminin components were solubilized from basement membranes of amnion, chorion and chorionic microvessels of human placenta without prior protease digestion. These structures, after isolation, were initially processed in a sonicator bath containing a solution of Triton X-100, EDTA and 2M NaCl and the laminins extracted sequentially with 0.5M NaCl, 8 M urea, and 8 M urea + 2% 2-mercaptoethanol + 2% SDS. A high molecular weight (appr. 1 x 10(6)) complex containing laminins was purified by gel filtration on a Sepharose CL-2B column. This complex migrated as a single band on gel electrophoresis before reduction but resolved, after reduction, into four major laminin components, laminin A (350,000 M.W.), laminin M (240,000 M.W.) and laminins B1 and B2 (195,000 and 185,000 M.W.). Laminin M is a new molecular species of this protein.
mesh_terms: D000596:Amino Acids / Q000302:isolation & purification; D000650:Amnion / Q000032:analysis*; D000818:Animals; D001485:Basement Membrane / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002823:Chorion / Q000032:analysis* / Q000098:blood supply; D005260:Female; D006023:Glycoproteins / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D007120:Immunochemistry; D007797:Laminin; D008565:Membrane Proteins / Q000302:isolation & purification*; D008833:Microcirculation / Q000032:analysis; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy; D051381:Rats; D012995:Solubility
chemical_list: D000596:Amino Acids; D006023:Glycoproteins; D007797:Laminin; D008565:Membrane Proteins