Gel-exclusion chromatography on S1000 Sephacryl: application to phospholipid vesicles.
Sephacryl S1000 has an exclusion diameter of approximately 3000 A and is thus an appropriate gel exclusion medium for size analysis and fractionation of phospholipid vesicles. Calibration is conveniently carried out using polystyrene beads of known diameter eluted with a detergent-containing buffer. Recovery of phospholipid vesicles of different sizes from S1000 presaturated with lipid is greater than 95%, and diameters obtained from a calibration curve using the polystyrene beads are in good agreement with those obtained by negative contrast electron microscopy.
D000180:Acrylic Resins; D002850:Chromatography, Gel; D010316:Particle Size; D010743:Phospholipids / Q000302:isolation & purification*
D000180:Acrylic Resins; D010743:Phospholipids; C037239:Sephacryl Superfine