title: Laser Raman studies of the 5 S rRNA-protein L5 complex of rat liver ribosomes.
abstract: The effects of ribosomal protein L5 on the conformation of 5 S rRNA in the 5 S rRNA-protein L5 complex extracted from rat liver ribosomes have been studied by laser Raman spectroscopy. A comparison of the spectra shows small protein-induced conformational changes in the 5 S rRNA, but most of the base-paired regions appear to be present in the complex with protein L5 as well as in the free 5 S rRNA. Furthermore specific interactions between 5 S rRNA and protein L5 are indicated. Cytosine (and/or uracil) residues in single-stranded regions and the N(7) of guanine are engaged in interactions with the protein as suggested by the Raman data.
mesh_terms: D000818:Animals; D008099:Liver / Q000032:analysis*; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal / Q000302:isolation & purification*; D051381:Rats; D012269:Ribosomal Proteins / Q000302:isolation & purification*; D012270:Ribosomes / Q000032:analysis*; D013059:Spectrum Analysis, Raman
chemical_list: D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; C029742:ribosomal protein L5