The incorporation of O6-methyldeoxyguanosine and O4-methyldeoxythymidine monophosphates into DNA by DNA polymerases I and alpha.
The modified nucleoside 5'-triphosphates O6-MedGTP ad O4-MedTTP have been synthesised and their acceptability as DNA-precursors investigated using DNA polymerases I and alpha in an in vitro assay. O6-MedGMP is only incorporated into newly-synthesized DNA-like material in the presence of templates containing thymine bases. Similarly O4-MedTMP is only incorporated in the presence of templates containing guanine bases. The results confirm the promutagenic nature and base-pairing properties of O6-MeG ad O4-MeT.
D004256:DNA Polymerase I / Q000378:metabolism*; D004257:DNA Polymerase II / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003848:Deoxyguanine Nucleotides / Q000378:metabolism* / Q000494:pharmacology; D013938:Thymidine Monophosphate / Q000378:metabolism*; D013942:Thymine Nucleotides / Q000378:metabolism* / Q000494:pharmacology
D003848:Deoxyguanine Nucleotides; D013942:Thymine Nucleotides; D013938:Thymidine Monophosphate; C018601:O(6)-methyldeoxyguanylic acid; C023827:O(6)-methyldeoxyguanosine triphosphate; C038566:O(4)-methylthymidine monophosphate; C038567:O(4)-methylthymidine triphosphate; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D004259:DNA-Directed DNA Polymerase