title: [Incorporation of L-acetidine-2-carboxylic acid in type I skin collagen. Biochemical and mechanoelastic properties].
abstract: In order to elucidate the role of L-acetidine-2-carboxylic acid (L-ac) incorporated into collagen type I of the skin instead of proline, we looked for the mechanoelastic properties of skin. Mice were orally fed with 0, 1% solution of L-ac for 5 weeks, then sacrificed, and type I collagen was extracted. Incorporation of the proline analogue (L-ac) could be shown by two dimensional thinlayer chromatography. The melting point (Tm) of type I collagen was determined by circular dichroism: 36, 5 +/- 1 degrees C for normal collagen, 37 +/- 1 degrees C for L-ac-collagen. This insignificant difference indicates that there was no alteration of the thermal stability of the collagen helix after incorporation of L-ac. Tensile strength was examined on whole strips of skin and worked out at means = 1,00 N/mm, s = 0,20 N/mm, sigma = 0,04 N/mm for normal individuals and means = 0,62 N/mm, s = 0,24 N/mm, sigma = 0,05 N/mm for L-ac-fed animals. The considerable difference could be estimated by the Wilcoxon test (p less than 0,005). As the stability of the collagen helix (shown through melting point determination) has not decreased, the reduced mechanoelastic property of tensile strength seems to be due to intermolecular rather than to intramolecular disturbances of the cross connection of the triple helix.
mesh_terms: D000818:Animals; D001383:Azetidinecarboxylic Acid / Q000378:metabolism* / Q000494:pharmacology; D001385:Azetines / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D003094:Collagen / Q000096:biosynthesis / Q000378:metabolism*; D051379:Mice; D011392:Proline / Q000031:analogs & derivatives*; D012867:Skin / Q000378:metabolism*; D013718:Tensile Strength
chemical_list: D001385:Azetines; D001383:Azetidinecarboxylic Acid; D003094:Collagen; D011392:Proline
vernacular_title: Einbau von L-Acetidin-2-Carbonsäure in Typ I Kollagen der Haut. Biochemische und mechanische Veränderungen.