Characterization of lactose transport in Kluyveromyces lactis.
We have determined that lactose uptake in Kluyveromyces lactis is mediated by an inducible transport system. Induction, elicited by lactose or galactose, of the transporter required protein synthesis. Transport of lactose required an energy-generating system and occurred by an active process, since an intracellular lactose concentration 175 times greater than the extracellular concentration could be obtained. The Km for lactose transport was about 2.8 mM in uninduced and lactose- or galactose-induced cells. The lactose transporters in K. lactis and Escherichia coli appear to be different since they respond uniquely to inhibition by substrate analogs.
D019297:2,4-Dinitrophenol; D001203:Ascomycota / Q000378:metabolism*; D001386:Azides / Q000494:pharmacology; D001693:Biological Transport, Active; D004140:Dinitrophenols / Q000494:pharmacology; D004790:Enzyme Induction; D029968:Escherichia coli Proteins; D005656:Fungal Proteins / Q000096:biosynthesis; D005690:Galactose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007785:Lactose / Q000378:metabolism* / Q000494:pharmacology; D026901:Membrane Transport Proteins / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins; D004718:Saccharomycetales / Q000201:enzymology / Q000378:metabolism*; D019810:Sodium Azide; D027981:Symporters
D001386:Azides; D004140:Dinitrophenols; D029968:Escherichia coli Proteins; D005656:Fungal Proteins; C050221:LacY protein, E coli; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D027981:Symporters; C021499:lactose permease; D019810:Sodium Azide; D007785:Lactose; D019297:2,4-Dinitrophenol; D005690:Galactose