Measurement of lipoprotein lipase and hepatic triacylglycerol lipase in post-heparin plasma of the cynomolgus monkey.
Conditions for measurement of the lipolytic activities, lipoprotein lipase and hepatic triacylglycerol lipase in cynomolgus monkey postheparin plasma are described. The two activities are separable by heparin-Sepharose chromatography. Goat anti-human hepatic triacylglycerol lipase serum inhibits monkey hepatic triacylglycerol lipase activity and allows direct measurement of lipoprotein lipase in post-heparin plasma. While both human and homologous serum can be used as a source of activator apolipoprotein, homologous serum produces a much greater activation.
D000818:Animals; D005260:Female; D006041:Goats; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007106:Immune Sera / Q000032:analysis; D008049:Lipase / Q000097:blood*; D008071:Lipoprotein Lipase / Q000097:blood*; D008099:Liver / Q000378:metabolism*; D008252:Macaca fascicularis; D008297:Male
D007106:Immune Sera; D006493:Heparin; D008049:Lipase; D008071:Lipoprotein Lipase