Octamer reconstitution from acid-extracted chicken erythrocyte histones.
Histone octamers have been reconstituted from acid-extracted chicken erythrocyte histones. By the criteria of molecular size on exclusion chromatography as well as sedimentation velocity and conformational properties established by circular dichroism, fluorescence spectroscopy and imido-ester cross-linking, the reconstituted octamers have a structure identical to that of salt-extracted octamers.
D000818:Animals; D001704:Biopolymers; D002645:Chickens; D002845:Chromatography / Q000379:methods; D004912:Erythrocytes / Q000378:metabolism*; D006657:Histones / Q000097:blood / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D013057:Spectrum Analysis / Q000379:methods
D001704:Biopolymers; D006657:Histones