An investigation of high-performance liquid chromatographic methods for the analysis of amphetamines.
Three pre-column derivatization reagents, namely o-phthalaldehyde, 4-chloro-7-nitro-benz-2,1,3-oxadiazole, sodium naphthaquinone-4-sulphonate and two ion-pair reagents, namely, naphthalene-2-sulphonate and sodium dodecylsulphate have been investigated for their suitability for the qualitative and quantitative analysis of urine and plasma samples containing amphetamines. The derivatization method employing sodium naphthaquinone-4-sulphonate was found to be selective and sufficiently sensitive for the routine determination of amphetamine and methylamphetamine in urine and plasma samples at the ng/ml level.
D000328:Adult; D000662:Amphetamines / Q000032:analysis* / Q000097:blood / Q000652:urine; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008297:Male
D000662:Amphetamines