Occurrence of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline in tissues and body fluids of rat.
A capillary column gas chromatographic-mass spectrometric method was used to identify and quantitate 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline (6OMTHBC) in rat. The excretion rate in urine was 0.73 +/- 0.20 nmoles per 24 hr and in faeces 0.18 +/- 0.03 nmoles per 24 hr. In urine, about 90% of the 6OMTHBC was in a conjugated form, whereas in faeces most (approximately 75%) of the 6OMTHBC was in a free form. The compound was detectable in liver (11.1 +/- 3.6 pmoles/g), kidney (2.1 +/- 0.9 pmoles/g) and plasma (0.52 +/- 0.15 pmoles/ml), but not in brain (less than 0.3 pmoles/g). When 6OMTHBC was injected to rats, 75% of the injected amount was excreted in urine during the first 10 hr. The plasma level of 6OMTHBC declined with a half-life of 1.5 hr.
D000818:Animals; D002243:Carbolines / Q000097:blood / Q000378:metabolism* / Q000652:urine; D005243:Feces / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007211:Indoles / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D002243:Carbolines; D007211:Indoles; C035602:6-hydroxy-1-methyltryptoline