Inhibition of phagocytosis by erythrocyte membrane sialoglycoprotein on target liposomes.
Sialoglycoprotein (GP) of human erythrocytes was incorporated into liposomes and its effect on the Fc receptor-mediated phagocytic reaction of human PMN cells was examined. Whereas liposomes carrying 2,4-dinitrophenylated lipid were, upon opsonization with rabbit anti-DNP, readily ingested by PMN cells and induced the NBT-reducing reaction, these reactions were markedly suppressed when GP was incorporated into the target liposomes. The inhibitory activity was found in the glycophorin A and B fractions, but the latter was more active than the former on a weight basis. It was estimated that incorporation of only a single molecule of GP per vesicle of 6000 lipid molecules may be sufficient to protect the particle from phagocytosis, but there was an apparent antagonism between the suppressive GP and opsonizing antibody as, with more antibody, more GP became necessary to inhibit phagocytosis. The effect of GP was largely abolished by trypsin treatment of GP-bearing liposomes or by the addition of F(ab')2 of anti-GP.
D004306:Dose-Response Relationship, Immunologic; D006021:Glycophorins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007165:Immunosuppression Therapy; D008081:Liposomes / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects*; D010714:Phosphatidylethanolamines / Q000276:immunology; D012795:Sialoglycoproteins / Q000494:pharmacology*; D014357:Trypsin / Q000494:pharmacology
C021015:2,4-dinitrophenyl-6-N-aminocaproylphosphatidylethanolamine; D006021:Glycophorins; D007140:Immunoglobulin Fab Fragments; D008081:Liposomes; D010714:Phosphatidylethanolamines; D012795:Sialoglycoproteins; D014357:Trypsin