[Two types of attachment points of DNA to the nuclear skeleton in Ehrlich ascites carcinoma cells].
Two types of DNA fragments attached to the nuclear skeleton and protected from staphylococcal nuclease digestion by the nuclear skeleton's proteins were found in Ehrlich ascites carcinoma cells: (i) long (approximately 10 kb) satellite DNA fragments and (ii) short (approximately 140 b. p.) main band DNA fragments. The amount of short DNA fragments depends strongly on the conditions of nuclei isolation. In the preparations obtained from the cells lysed in the medium lacking Cu2+ or Zn2+ short DNA fragments were not found. On the contrary long satellite DNA fragments were found in presumably equal amounts in the nuclei obtained by a wide variety of experimental procedures. Different stability of the structures providing attachment and protection from nuclease digestion of long and short DNA fragments seem to be a good reasons to suggest the existence of two types of attachment points of DNA to the nuclear skeleton.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000378:metabolism*; D003852:Deoxyribonucleoproteins / Q000302:isolation & purification / Q000378:metabolism; D004355:Drug Stability; D051379:Mice; D008836:Micrococcal Nuclease; D008970:Molecular Weight; D009698:Nucleoproteins / Q000378:metabolism*; D011485:Protein Binding
D004273:DNA, Neoplasm; D003852:Deoxyribonucleoproteins; D009698:Nucleoproteins; D008836:Micrococcal Nuclease
Dva tipa uchastkov prikrepleniia DNK k iadernomu skeletu v kletkakh astsitnoĭ kartsinomy Erlikha.