5-methylcytosine content in different organs of the mouse and rat, several tumors and in mice embryos.
1. The content of 5-methylcytosine of the DNA of 11-day old mice embryos was compared with that of the DNA of different plasmocytoms. 2. Furthermore the DNA content was determined in different transplantable tumors and normal organs of the rat and the mouse. 3. By the aid of liquid chromatography significant differences could be demonstrated in the content of 5-methylcytosine of the DNA of BALB/c-mice or NMRI-mice and the DNA of the plasmocytom MOPC 104 E and MOPC 141, but not in the case of MOPC 321. 4. Clear differences could be observed with restriction enzymes: the DNA of all the plasmocytoms studied is more digested with Hpa II and Hha I than the DNA of the embryos. This is not the case however with MSP I. 5. The DNA of three transplantable tumors tested contains less 5-methylcytosine than the one of the liver kidney of the mouse and the rat. This was confirmed with restriction enzymes too.
D044503:5-Methylcytosine; D000818:Animals; D003596:Cytosine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D004247:DNA / Q000302:isolation & purification*; D004273:DNA, Neoplasm / Q000302:isolation & purification; D004622:Embryo, Mammalian / Q000032:analysis; D005260:Female; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D051379:Mice; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000032:analysis; D010954:Plasmacytoma / Q000032:analysis; D011247:Pregnancy; D051381:Rats
D004273:DNA, Neoplasm; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA