Buoyant densities of macromolecules, macromolecular complexes, and cell organelles in Nycodenz gradients.
Nycodenz is a new nonionic iodinated density gradient medium which has several advantages over metrizamide. Although, overall, biological samples band at similar densities in Nycodenz and metrizamide gradients, a number of significant differences were found. As compared with metrizamide, not only does Nycodenz appear to interact less with proteins but also the buoyant density of chromatin is less affected by the amount loaded onto the gradient. A high degree of resolution is obtainable using Nycodenz gradients; thus, it is possible to separate density-labeled DNA and to subfractionate subcellular membrane fractions.
D000818:Animals; D002499:Centrifugation, Density Gradient / Q000379:methods; D004247:DNA / Q000302:isolation & purification; D006168:Guinea Pigs; D007463:Iodobenzoates; D007472:Iohexol; D008099:Liver / Q000648:ultrastructure*; D046911:Macromolecular Substances / Q000032:analysis; D008793:Metrizamide; D009698:Nucleoproteins / Q000032:analysis*; D051381:Rats; D013046:Specific Gravity; D013347:Subcellular Fractions / Q000032:analysis*; D013395:Sucrose; D014283:Triiodobenzoic Acids
D007463:Iodobenzoates; D046911:Macromolecular Substances; D009698:Nucleoproteins; D014283:Triiodobenzoic Acids; D007472:Iohexol; D013395:Sucrose; D004247:DNA; D008793:Metrizamide