Determination of fluphenazine in plasma by high-performance thin-layer chromatography.
Determination of fluphenazine in blood plasma by in situ fluorescent detection after separation by high-performance thin-layer chromatography is described. Enhancement of fluorescent emission of the drug is accomplished by exposure to UV light in the presence of paraffin oil which permits a limit of detectability of approximately 0.1 ng/ml in blood plasma. Thirty samples or more can be processed in a 7-h period with excellent precision (less than 3% relative standard deviation at 2.5 ng/ml). Investigation of extraction procedures, chromatographic conditions, photodevelopment, and fluorescent detection are described.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D005453:Fluorescence; D005476:Fluphenazine / Q000097:blood* / Q000302:isolation & purification / Q000627:therapeutic use; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013053:Spectrophotometry; D013997:Time Factors
D005476:Fluphenazine