Preparation and metabolism of 125I-sulfobromophthalein.
Metabolism of 125I-sulfobromophthalein (BSP) prepared by the chloramine-T method was studied in rats. 125I-BSP is removed rapidly from the circulation. However, as compared to BSP, its plasma clearance and biliary excretion are delayed, and its accumulation in the liver is prolonged. Although BSP and 125I-BSP show similar binding to albumin in serum, their binding properties to liver cytosolic proteins and to the liver cell plasma membrane organic anion binding protein (OABP) differ. In contrast to the X-, Y- and Z-protein binding of BSP, 125I-BSP binds predominantly to a high molecular weight protein and only a small proportion of 125I-BSP binds to OABP.
D000818:Animals; D001646:Bile / Q000378:metabolism; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D008099:Liver / Q000378:metabolism; D008297:Male; D008657:Metabolic Clearance Rate; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000378:metabolism; D013448:Sulfobromophthalein / Q000097:blood / Q000378:metabolism*
D007457:Iodine Radioisotopes; D012709:Serum Albumin; D013448:Sulfobromophthalein