Cystathionase: a potential cytoplasmic marker of hematopoietic differentiation.
Cysteine auxotrophy and absence of cystathionase (CSE) has been associated with certain human and rodent leukemic cell lines. To determine whether this state was a marker of malignant transformation or of cellular differentiation, CSE content was measured in 16 well characterized human leukemia-lymphoma cell lines. Enzyme was easily detected in several lines but its level did not correlate with a proposed scheme of differentiation based on cell-surface markers. However, the apparent absence of enzyme in human bone marrow CFU-C determined by growth experiments suggests reduced levels of CSE may be a marker of cytoplasmic immaturity.
D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002460:Cell Line; D003114:Colony-Forming Units Assay; D003540:Cystathionine / Q000378:metabolism; D003542:Cystathionine gamma-Lyase / Q000378:metabolism*; D003545:Cysteine / Q000378:metabolism; D003593:Cytoplasm / Q000201:enzymology*; D006412:Hematopoietic Stem Cells / Q000201:enzymology; D006801:Humans; D007938:Leukemia / Q000201:enzymology*; D008190:Lyases / Q000378:metabolism*
D003540:Cystathionine; D008190:Lyases; D003542:Cystathionine gamma-Lyase; D003545:Cysteine