title: Seminalplasmin inhibits transcription and translation of phi80 DNA in vitro.
abstract: Seminalplasmin, an antimicrobial protein from bovine seminal plasma that has been earlier shown to inhibit transcription in whole cells and by purified RNA polymerase in vitro, but not translation in whole cells, is now shown to inhibit both transcription and translation independently of each other, in a coupled transcription-translation system from E. coli using phi80dphoAlacZ DNA as the template.
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D002474:Cell-Free System; D003090:Coliphages; D004279:DNA, Viral / Q000235:genetics; D005786:Gene Expression Regulation; D014176:Protein Biosynthesis / Q000187:drug effects*; D011506:Proteins / Q000494:pharmacology*; D012661:Semen / Q000382:microbiology*; D029607:Seminal Vesicle Secretory Proteins; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D004279:DNA, Viral; D011506:Proteins; D029607:Seminal Vesicle Secretory Proteins