A gene involved in action of tumor promoters is identified and mapped in Caenorhabditis elegans.
We isolated mutants of the nematode Caenorhabditis elegans resistant to 12-O-tetradecanoylphorbol-13-acetate (TPA). The TPA-resistant mutants, although they grew somewhat smaller than normal, reproduced well and behaved normally in TPA; they also did similarly in another phorbol ester tumor promoter, phorbol-12,13-didecanoate (PDD), thus proving they are also resistant to PDD. All the mutations defined by these TPA-resistant mutants were semidominant to the wild-type allele. The 15 independently isolated mutants all fell into the same complementation group, defining a single gene, tpa-1. The gene tpa-1 mapped near the marker gene dpy-9 on chromosome IV.
D000818:Animals; D002107:Caenorhabditis / Q000235:genetics*; D002874:Chromosome Mapping; D004351:Drug Resistance; D005796:Genes / Q000187:drug effects*; D009154:Mutation; D010703:Phorbol Esters / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D010703:Phorbol Esters; C008876:phorbol-12,13-didecanoate; D013755:Tetradecanoylphorbol Acetate