Kinetic assay of aldehyde oxidase with 2,2'-azino-di-(3-ethylbenzthiazoline-6-sulfonate) as chromogen.
A new kinetic method for the assay of aldehyde oxidase (EC 1.2.3.1) is described. By the oxidation of pyridoxal as a substrate, pyridoxic acid and hydrogen peroxide are formed. Hydrogen peroxide is subsequently reduced to water by the action of peroxidase, while the chromogen 2,2'-azino-di-(3-ethylbenzthiazoline-6-sulfonate) (ABTS) is oxidized to a blue-greenish-dyed product. The absorbance increase of the oxidized form of ABTS, registered every minute at 410 nm, is proportional to aldehyde oxidase activity.
D042931:Aldehyde Oxidase; D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D000818:Animals; D052160:Benzothiazoles; D002374:Catalase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D011817:Rabbits; D013451:Sulfonic Acids
D052160:Benzothiazoles; D007202:Indicators and Reagents; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D002374:Catalase; D000445:Aldehyde Oxidoreductases; D042931:Aldehyde Oxidase