Liquid-chromatographic determination of aminoglutethimide in plasma.
A simple, rapid "high-performance" liquid-chromatographic procedure is presented for the determination of aminoglutethimide in plasma. After precipitation of the protein with acetonitrile, an aliquot of the supernate is injected directly onto a radially compressed, reversed-phase column. The aminoglutethimide is isocratically eluted with a mobile phase of acetonitrile/water/tert-butyl ammonium phosphate. The method is both accurate and precise and has been in routine use in our laboratory for more than 12 months.
D000616:Aminoglutethimide / Q000097:blood* / Q000302:isolation & purification; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
D000616:Aminoglutethimide