Chemical identification of the vasopressin immunoreactive materiaL present in the rat suprachiasmatic nucleus.
Whole tissue homogenates and acid-ethanol extracts of rat suprachiasmatic nuclei were subjected to SDS-gel electrophoresis and gel isoelectric focusing, respectively. Fractions were tested for vasopressin (VP) immunoreactivity by means of radioimmunoassay (RIA). SDS-gel electrophoresis showed one distinct Vp immunoreactive peak in the peptide range (Mr less than 10,000). Isoelectric focusing revealed one VP immunoreactive peak as well, having the same pH position as synthetic VP. These results further substantiate the presence of VP-containing neurons in the suprachiasmatic nucleus.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007525:Isoelectric Focusing / Q000379:methods; D008297:Male; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D013493:Suprachiasmatic Nucleus / Q000032:analysis*; D014667:Vasopressins / Q000032:analysis*
D014667:Vasopressins