title: Non-selenium-dependent glutathione peroxidase activity in rat lung: association with lung glutathione S-transferase activity and the effects of hyperoxia.
abstract: To determine if non-selenium-dependent glutathione peroxidase (Non-Se GSH-Px) activity is present in rat lung, we fractionated rat lung soluble fractions from rats fed a selenium-deficient or control diet and measured glutathione peroxidase activity with both cumene hydroperoxide and hydrogen peroxide as substrates. We also measured glutathione S-transferase (GSH S-transferase) activity in the fractions with 1-chloro-2,4-dinitrobenzene as substrate. Non-Se GSH-Px activity was present (about 34% of total GSH-Px activity), and the peak present in the gel filtration chromatogram coeluted with the GSH S-transferase peak. We then measured GSH S-transferase activity in lung-soluble fractions from rats exposed to room air or 85% O2 for 5 days. Lung GSH S-transferase activity was increased in the oxygen-exposed animals when compared to the air-exposed controls. The increase in GSH S-transferase activity could represent the induction of lung non-Se GSH-Px activity.
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D004789:Enzyme Activation / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D008168:Lung / Q000201:enzymology*; D008297:Male; D010100:Oxygen / Q000494:pharmacology*; D010544:Peroxidases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012643:Selenium / Q000172:deficiency
chemical_list: D010544:Peroxidases; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D012643:Selenium; D010100:Oxygen