Affinity chromatography of acetylcholinesterase from Electrophorus electricus electroplax. Investigations on 9-aminoalkylacridine affinity ligands.
The neural enzyme 11-S acetylcholinesterase (E.C. 3.1.1.7) was purified by affinity chromatography from a trypsin digest of Electrophorus electricus electric organ. Unquaternized affinity ligands were reported which were comparable in efficacy to the routinely employed "quaternized acridine MAC ligand". A study was made of the quaternization reactions of various 9-aminoalkylacridines and 9-aminoacridine along with their relative binding affinities to acetylcholinesterase. Ease of synthesis in conjunction with the column performance of these unquaternized 9-aminoalkylacridine compounds made them the preferred affinity ligand in acetylcholinesterase chromatography. A new carbodiimide synthetic route for these unquaternized ligands was described.
D000110:Acetylcholinesterase / Q000302:isolation & purification*; D000609:Aminoacridines; D000818:Animals; D002846:Chromatography, Affinity; D004557:Electric Organ / Q000201:enzymology*; D004593:Electrophorus; D008024:Ligands
D000609:Aminoacridines; D008024:Ligands; D000110:Acetylcholinesterase