Identification and characterization of variant forms of the gastrin-releasing peptide (GRP).
Porcine intestinal gastrin-releasing peptide (GRP) has been demonstrated to be structurally identical to the previously characterized gastric GRP. Ion-exchange and high-performance liquid chromatography of porcine intestinal extracts have identified two variant GRP forms. Studies on one of these variant forms suggest that a beta-aspartyl shift has occurred in the Asn-His structure of GRP; such a modification in an Asn-His structure occurring in a natural peptide or protein has not been previously reported. This variant GRP, although retaining bioactivity, appears to have reduced potency in elevating canine plasma gastrin levels.
D000595:Amino Acid Sequence; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D019886:Gastrin-Releasing Peptide; D005755:Gastrins / Q000378:metabolism; D006168:Guinea Pigs; D007422:Intestines / Q000032:analysis*; D010455:Peptides / Q000145:classification / Q000302:isolation & purification* / Q000494:pharmacology; D013552:Swine
D005755:Gastrins; D010455:Peptides; D019886:Gastrin-Releasing Peptide