Intracellular topography of immunoreactive gastrin demonstrated using electron immunocytochemistry.
Gastrin (G)-producing cells from the mammalian gastric antrum have been investigated using computer-assisted morphometry and a novel double colloidal gold-labeled-immunoglobulin electron immunocytochemical procedure. Correlation analysis of human antral G-cells indicates (p less than 0.001) that a single population of granules exists with small (160 nm) electron-dense and large (240 nm) electron-lucent forms representing the extremes. Non-crossreacting region-specific antisera have been used to visualize G-17 and G-34 (progastrin) to the small electron-dense granules and G-17 to the other intermediate forms. From the results we propose a topographic segregation of immunoreactive gastrins within 2 apparently distinct granule subclasses and suggest that this may represent the pathway of granule maturation.
D000328:Adult; D000818:Animals; D002415:Cats; D005753:Gastric Mucosa / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D005755:Gastrins / Q000276:immunology / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D007120:Immunochemistry; D008854:Microscopy, Electron / Q000379:methods; D011706:Pyloric Antrum / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure
D005755:Gastrins