Analysis of erythrocyte membrane proteins from dystrophic hamsters.
To search for potentially mutant proteins, we have investigated erythrocyte ghost proteins from normal and dystrophic hamster by two-dimensional gel electrophoresis. No significant differences are observed between dystrophic and normal erythrocytes in their peptide patterns on SDS-polyacrylamide gel electrophoresis while on two-dimensional gels a protein spot of approximate Mr 20 000 with an approximate isoelectric point of 4.5 is found in erythrocytes from dystrophic animals and is consistently absent in normal erythrocytes. A large population of erythrocyte (60%) from dystrophic hamsters shows distorted shape as visualized by scanning electron microscopy. The nature of this protein and its relevance in hamster muscular dystrophy are at present not known.
D000818:Animals; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000032:analysis* / Q000648:ultrastructure; D004912:Erythrocytes / Q000032:analysis*; D007526:Isoelectric Point; D008565:Membrane Proteins / Q000097:blood*; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D009137:Muscular Dystrophy, Animal / Q000097:blood*
D008565:Membrane Proteins