Genetic analysis of the T-H-2 region in non-t chromosomes. I. Two new congenic strains isolate Qglo-1 from H-2.
A general breeding protocol useful in the construction of congenic lines of mice disparate in the 15 cM T-H-2 region of chromosome 17 in non-t chromosomes is described. Two such congenic lines, B6.TC2/Rn and B6.TC3/Rn, were derived from the C57BL/6J and B6.C-H-2d/By strains using this protocol. Both B6.TC2 and B6.TC3 dissociate the quantitative activity locus for glyoxalase I (Qglo-1) from the H-2 complex, and hence possess BALB/cBy DNA centromeric to H-2. However, neither new strain is able to map any H-2-associated restriction fragment length polymorphism with an H-2 cDNA probe even though both strains are recombinant in the 2 cM Qglo-1-H-2K interval.
D000818:Animals; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D005796:Genes; D008040:Genetic Linkage; D006183:H-2 Antigens / Q000235:genetics*; D007791:Lactoylglutathione Lyase / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics; D008810:Mice, Inbred C57BL / Q000235:genetics; D008815:Mice, Inbred Strains / Q000235:genetics*
D006183:H-2 Antigens; D004247:DNA; D007791:Lactoylglutathione Lyase