Method for enzymatic determination of imidazole acetic acid.
A method for enzymatic assay of imidazole acetic acid (ImAA) was developed, based on the strict substrate specificity of imidazole acetate monooxygenase from Pseudomonas sp. [Maki et al. (1969) J. Biol. Chem., 244., 2942-2950], which catalyzes concomitant conversion of NADH to NAD+. Thus, ImAA was determined by measuring decrease in absorbancy at 340 nm. Tissue extracts were partially purified and/or concentrated by column chromatography on Bio-Rad AG-1 before enzymatic assay. The lowest measurable level of ImAA by this method was 2 nmol.
D000818:Animals; D005260:Female; D007093:Imidazoles / Q000032:analysis* / Q000097:blood / Q000652:urine; D008297:Male; D051379:Mice; D006899:Mixed Function Oxygenases / Q000378:metabolism; D010920:Placenta / Q000032:analysis; D011247:Pregnancy; D011549:Pseudomonas / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
D007093:Imidazoles; D006899:Mixed Function Oxygenases; C038252:imidazoleacetate 4-monooxygenase; C005954:imidazoleacetic acid