Demonstration of fluid-phase endocytosis in epithelial cells of the male reproductive system by means of horseradish peroxidase-colloidal gold complex.
The occurrence of fluid-phase endocytosis in Sertoli cells as well as epithelial cells of the rete testis and ductuli efferentes was demonstrated by use of horseradish peroxidase combined with colloidal gold (HRP-G) as a tracer. The characteristic electron dense spherical colloidal gold particles facilitate the localization of the HRP-G complex in pinocytotic vesicles, multivesicular bodies, and lysosomes of these epithelial cells. With this method, which does not require the use of diaminobenzidine, a clear distinction can be made between peroxidases from endogenous and exogenous origins.
D000818:Animals; D003102:Colloids; D004705:Endocytosis; D004848:Epithelium / Q000648:ultrastructure*; D006046:Gold; D006735:Horseradish Peroxidase; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D012152:Rete Testis / Q000648:ultrastructure; D012708:Sertoli Cells / Q000648:ultrastructure; D013737:Testis / Q000648:ultrastructure*
D003102:Colloids; D006046:Gold; D006735:Horseradish Peroxidase