High pressure liquid chromatographic assay for prednisone in bulk drug substances and tablets.
A high pressure liquid chromatographic (HPLC) method was developed for the assay of prednisone in bulk drug substances and tablets. The sample was dissolved in water-methanol and an aliquot was analyzed by using HPLC. The average recovery of prednisone added to a prednisone tablet composite was 99.5% with a coefficient of variation of 1.07%. Prednisone was determined in 46 tablets (1-50 mg prednisone/tablet) formulated by 22 manufacturers, using the HPLC method and the USP blue tetrazolium assay. The results show that the HPLC method is more specific and faster than the USP method.
D002851:Chromatography, High Pressure Liquid; D011241:Prednisone / Q000032:analysis* / Q000592:standards; D013607:Tablets / Q000032:analysis
D013607:Tablets; D011241:Prednisone