Response of human aldosteronoma cells in culture to the N-terminal glycopeptide of pro-opiomelanocortin and gamma 3-MSH.
Cells were isolated from one of two adenomas from the adrenal cortex of a hypertensive patient with primary hyperaldosteronism. A primary culture of these cells responded with increased aldosterone secretion to gamma 3-MSH and human (residues 1-76) and porcine (residues 1-80) N-terminal peptides of pro-opiomelanocortin (POMC). EC50 was lowest for gamma 3-MSH, while maximal response was greater for both N-terminal peptides of POMC. We conclude that gamma 3-MSH derived from the N-terminal segment of POMC contains the active core of the aldosterone-stimulating activity of the N-terminal on these adrenal cells.
D000236:Adenoma / Q000150:complications / Q000378:metabolism*; D000306:Adrenal Cortex Neoplasms / Q000150:complications / Q000378:metabolism*; D000450:Aldosterone / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006020:Glycopeptides / Q000494:pharmacology*; D006801:Humans; D006929:Hyperaldosteronism / Q000150:complications / Q000378:metabolism*; D066298:In Vitro Techniques; D009074:Melanocyte-Stimulating Hormones / Q000494:pharmacology*; D008875:Middle Aged; D010446:Peptide Fragments; D011333:Pro-Opiomelanocortin; D013552:Swine
D006020:Glycopeptides; D010446:Peptide Fragments; C031562:pro-opiomelanocortin amino-terminal glycopeptide; D000450:Aldosterone; D011333:Pro-Opiomelanocortin; D009074:Melanocyte-Stimulating Hormones