A homogeneous sol particle immunoassay for human chorionic gonadotrophin using monoclonal antibodies.
A mixture of monoclonal antibodies against human chorionic gonadotrophin (HCG) from 2 different clones was used to develop a homogeneous sol particle immunoassay (SPIA) for HCG, with high discrimination against luteinizing hormone (LH). The optimization of this assay is described. The work resulted in a spectrophotometric and a visual reading version. In an evaluation of the tests with 348 urines from pregnant women, 530 urines of non-pregnant women of fertile age and 100 post-menopausal women, the spectrophotometric screening test was 100% correct in the groups of urines from pregnant women and from post-menopausal women, and 99.8% correct in the group of urines from non-pregnant women of fertile age. This spectrophotometric screening test required an incubation period of 1 h and detected 280 IU/1 HCG. The eye reading test required an incubation period of 2 h and was able to detect about 450 IU/1 HCG. This test showed 100% correct results in the group of urines from non-pregnant women and from post-menopausal women, and 99.1% correct results in the group of pregnant women. In both test versions HCG concentrations up to 200,000 IU/1 did not result in false negative reactions. Urines of non-pregnant women, with an added 1000 IU/1 LH, gave correct negative test results.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D006063:Chorionic Gonadotropin / Q000032:analysis* / Q000276:immunology / Q000652:urine; D003102:Colloids; D003429:Cross Reactions; D005260:Female; D006046:Gold / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods / Q000592:standards; D007986:Luteinizing Hormone / Q000652:urine; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods
D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D003102:Colloids; D006046:Gold; D007986:Luteinizing Hormone