Comparative studies of the immunogenic activity of hepatitis B surface antigen (HBsAg) and HBsAg polypeptides.
Three hepatitis B surface antigen (HBsAg) preparations were compared: purified intact 22-nm HBsAg particles; HBsAg-derived, sodium dodecyl sulfate (SDS)-denatured P25 + GP30 polypeptide pool; and nondenatured P25 + GP30 micelles. The micelles had the same polypeptide composition as the P25 + GP30 pool. The immunogenicity in mice of each preparation, administered either in saline suspension or adsorbed to aluminum gel, was compared. The SDS-denatured polypeptides were less immunogenic than intact HBsAg particles, whereas the micelles were more immunogenic. High anti-HBs titers were observed in mice immunized with micelle preparations in either saline suspension or adsorbed to aluminum gel for as long as 200 days after a booster inoculation, administered 26 days after the primary dose.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D003102:Colloids; D006023:Glycoproteins / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000096:biosynthesis*; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000276:immunology*; D007114:Immunization; D007117:Immunization, Secondary; D051379:Mice; D008823:Micelles; D011489:Protein Denaturation; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D003102:Colloids; D006023:Glycoproteins; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D008823:Micelles; D014764:Viral Proteins