Synaptogenesis and myelination in dissociated cerebral microcarrier cell cultures.
A new approach for cultivating dissociated cerebral neurons is described. It is based on a rapid attachment of neurons to the DEAE-cellulose cylindrical MC and their subsequent interconnection to form cell-MC conglomerates. Intensive fiber growth followed by synaptogenesis and progressive myelin formation are indicative of optimal conditions of nutrition and oxygenation.
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001369:Axons / Q000648:ultrastructure; D001921:Brain / Q000502:physiology*; D002448:Cell Adhesion; D002478:Cells, Cultured; D005333:Fetus; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009186:Myelin Sheath / Q000502:physiology*; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure; D011901:Ranvier's Nodes / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D013569:Synapses / Q000502:physiology*
D000110:Acetylcholinesterase