title: Nuclease digestion patterns as a criterion for nucleosome orientation in the higher order structure of chromatin.
abstract: Nuclease digestion patterns have been used to discriminate between possible orientations of nucleosomes in the higher order structure of chromatin. Computer simulations were done assuming 3 basically different orientations of nucleosomes which include all proposed models for the '30 nm fibre'. It is found that only alternating exposure of consecutive nucleosomes can explain the DNase I and DNase II digestion patterns.
mesh_terms: D000818:Animals; D002843:Chromatin / Q000032:analysis*; D003201:Computers; D003851:Deoxyribonucleases / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000378:metabolism*
chemical_list: D002843:Chromatin; D009707:Nucleosomes; D003851:Deoxyribonucleases