Colorimetric estimation of vitamin B1 in pharmaceutical preparations.
A simple colorimetric method of estimation of thiamine from various pharmaceutical formulations is reported. Thiamine forms a yellow coloured complex with para-aminophenol in ammonia medium. The complex is extracted in chloroform (lambda max. 430 nm). The Beer's law is obeyed from 1.5 to 10 micrograms/ml.
D003124:Colorimetry / Q000379:methods; D007267:Injections; D012996:Solutions / Q000032:analysis; D013607:Tablets / Q000032:analysis; D013831:Thiamine / Q000032:analysis*
D012996:Solutions; D013607:Tablets; D013831:Thiamine