title: [Genetic integrity following cryopreservation].
abstract: The use of human cryosperm in artificial insemination raises the question of its safety with respect to genetic damage. As outlined here, the rate of congenital malformations among those newborn cannot be regarded as a reliable indicator for any genetic hazard. We have therefore studied the effect of deep freezing on 1. the rate of chromosomal aberrations in human lymphocytes, and 2. the number of gene mutations at the HGPRT locus, and 3. the frequency of sister chromatid exchanges in Chinese hamster ovary cells. In all three test systems no evidence of induced genetic damage after cryoconservation was observed.
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D006224:Cricetinae; D003412:Cricetulus; D005615:Freezing; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D009154:Mutation; D012662:Semen Preservation; D012854:Sister Chromatid Exchange
vernacular_title: Zur Frage der genetischen Integrität nach Kryokonservierung.