Accumulation of leukemic cell DNA strand breaks with adriamycin and cytosine arabinoside.
Adriamycin (doxorubicin) and cytosine arabinoside (Ara-C) induce single strand DNA breaks and DNA fragmentation. In view of the therapeutic efficacy of adriamycin in combination with Ara-C in the treatment of human acute non-lymphocytic leukemia, we have attempted to assess the accumulation of DNA strand breaks and DNA fragmentation with these agents by alkaline elution analysis. Although a less than additive enhancement was noted for the elution of pulse labelled, replicating DNA, the results demonstrate an additive effect of adriamycin and Ara-C on the elution of pre-labelled L1210 murine leukemia DNA.
D000818:Animals; D002478:Cells, Cultured; D003561:Cytarabine / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology*; D007939:Leukemia L1210 / Q000235:genetics*; D051379:Mice
D004273:DNA, Neoplasm; D003561:Cytarabine; D004317:Doxorubicin