Evolution of filipin-sterol complexes and intramembrane particle distribution during ciliogenesis.
Membrane events during ciliogenesis were studied in the quail oviduct cells. Two membrane domains differentiate during ciliogenesis: the ciliary necklace progressively composed of one to six rows of intramembranous particle (IMPs), and the shaft membrane poor in IMPs but characterized by a high content of sterol. Membrane sterol can be detected by the use of filipin which forms filipin-sterol complexes (f-c complexes) visible in electron microscopy on both freeze fracture replicas and thin sections. During ciliary growth, the necklace IMP rows are progressively organized in an area completely devoid of f-c complexes whereas the membrane of growing shaft shows a high density of f-c complexes except at the ciliary tip.
D000818:Animals; D002454:Cell Differentiation; D002462:Cell Membrane / Q000648:ultrastructure; D002784:Cholesterol / Q000032:analysis*; D002923:Cilia / Q000648:ultrastructure*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005372:Filipin; D005614:Freeze Fracturing; D007202:Indicators and Reagents; D010057:Oviducts / Q000166:cytology* / Q000187:drug effects; D011784:Quail
D007202:Indicators and Reagents; D004958:Estradiol; D005372:Filipin; D002784:Cholesterol