Toxic, DNA-damaging and mutagenic activity of epichlorohydrin on human cells cultured in vitro.
Epichlorohydrin (ECHH) highly inhibited the tritiated thymidine uptake by human lymphocytes cultured in vitro, although the corresponding cell viability was unaffected. Furthermore, it elicited unscheduled DNA synthesis, acting as a DNA-damaging agent after its metabolic activation. ECHH also showed a clear toxic and mutagenic activity toward a human epithelial-like cell line, causing a decrease in cell viability and an increase in mutants resistant to 0.05 Lf/ml of diphtheria toxin.
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002728:Chlorohydrins / Q000633:toxicity*; D004247:DNA / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects; D004167:Diphtheria Toxin / Q000037:antagonists & inhibitors; D004351:Drug Resistance; D004811:Epichlorohydrin / Q000633:toxicity*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D013936:Thymidine / Q000378:metabolism
D002728:Chlorohydrins; D004167:Diphtheria Toxin; D009153:Mutagens; D004811:Epichlorohydrin; D004247:DNA; D013936:Thymidine