3H-imipramine uptake into rat striatal slices and imipramine-induced 3H-dopamine efflux.
The effect of imipramine on spontaneous efflux of radiolabelled dopamine (DA) from slices of rat striatum was examined by a superfusion method. Imipramine at concentrations of 10 - 100 microM enhanced the efflux of DA accumulated in a high-affinity uptake system in a concentration-dependent manner. This efflux of 3H-DA was not affected by conditions (Ca2+-free medium, 100 microM bretylium and 30 microM tetrodotoxin) which inhibited the release of 3H-DA by electrical stimulation. Furthermore, this imipramine-induced 3H-DA efflux was temperature-dependent. The uptake of 3H-imipramine into striatal slices was determined. This uptake was concentration- and temperature-dependent and increased linearly. These results are discussed in relation to the hypothesis that 3H-DA efflux by imipramine is connected with uptake of imipramine.
D000042:Absorption; D000818:Animals; D001949:Bretylium Compounds / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D007099:Imipramine / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D013696:Temperature; D013779:Tetrodotoxin / Q000494:pharmacology; D014316:Tritium
D001949:Bretylium Compounds; D014316:Tritium; D013779:Tetrodotoxin; D007099:Imipramine; D002118:Calcium; D004298:Dopamine