Mechanisms of lysosomal enzyme secretion by human U-937 monocytes.
Lysosomal enzyme secretion was studied in U-937, a mononuclear phagocyte cell line. The secretion of the marker enzyme, beta-N-acetyl-D-glucosaminidase, was low when the cells were challenged with phagocytosable stimuli (asbestos, zymosan, opsonised zymosan). Ammonium chloride and cytochalasin B induced secretion over 24 h of incubation. Phorbol myristate acetate and F-methyl-leucyl-phenylalanine, did not have any effect on secretion. It is suggested that U-937 cells may provide a useful model for the study of mononuclear phagocyte secretory pathways.
D000118:Acetylglucosaminidase / Q000378:metabolism*; D000643:Ammonium Chloride / Q000494:pharmacology; D002460:Cell Line; D003571:Cytochalasin B / Q000494:pharmacology; D006596:Hexosaminidases / Q000378:metabolism*; D006801:Humans; D008222:Lymphokines / Q000494:pharmacology; D008247:Lysosomes / Q000201:enzymology*; D009000:Monocytes / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D009239:N-Formylmethionine / Q000031:analogs & derivatives / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009842:Oligopeptides / Q000494:pharmacology; D010587:Phagocytosis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D008222:Lymphokines; D009842:Oligopeptides; D000643:Ammonium Chloride; D003571:Cytochalasin B; D009239:N-Formylmethionine; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006596:Hexosaminidases; D000118:Acetylglucosaminidase; D013755:Tetradecanoylphorbol Acetate