The detachment, attachment and growth capacity of human glioma and glia cells in culture.
The detachment, attachment and growth were studied in malignant glioma and normal glia cells in culture, using the palladium-agarose cloning method. The number of cells in each individual clone was repeatedly counted throughout 10 days. All four glioma cell-lines studied detached and attached themselves at higher rates than the normal, diploid, glia cells. This dynamic behaviour seemed to be a general property of cultured, malignant, glioma cells. All the cell-lines contained clones with different properties. Some clones were growing, others were nearly constant in cell number and yet others decreased in cell number as a function of time. The differences between these types of clones were surprisingly sharp.
D002448:Cell Adhesion; D002455:Cell Division; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D002478:Cells, Cultured; D005910:Glioma / Q000473:pathology / Q000503:physiopathology*; D006801:Humans; D007700:Kinetics; D009457:Neuroglia / Q000166:cytology / Q000502:physiology*