Purification and characterization of guinea pig antithrombin III.
Antithrombin III (AT III) is a serine esterase inhibitor which regulates the activity of a number of coagulation factors. In this report, a three-step procedure for the purification of guinea pig AT III is described. This method, which involves affinity chromatography with heparin-Sepharose, ion exchange chromatography using DEAE-Sepharose, and Sephadex G-100 gel chromatography, results in purified, functionally active AT III with a 39% yield. The potential role of this molecule in the regulation of lymphocyte activation is discussed.
D000818:Animals; D000990:Antithrombin III / Q000302:isolation & purification* / Q000502:physiology; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography / Q000379:methods; D006168:Guinea Pigs; D007122:Immunoelectrophoresis; D011863:Radioimmunoassay; D013917:Thrombin / Q000037:antagonists & inhibitors
D000990:Antithrombin III; D013917:Thrombin