A rapid biuret assay for protein of whole fatty tissues.
A rapid biuret procedure is described which avoids the turbidity that occurs with protein analysis of intact fatty tissues. Recovery is complete and absorbancy linear with both concentration of the soluble crystalline serum albumin standard and the volume of homogenate of a variety of tissues. This method has been used successfully for the determination of protein concentrations of homogenates of whole rat heart, liver, kidney, brain, lung, and the following muscles: gastrocnemius, interior and exterior obliques, red and white vastus lateralis, and soleus.
D000273:Adipose Tissue / Q000032:analysis*; D000818:Animals; D001738:Biuret Reaction; D001923:Brain Chemistry; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D008168:Lung / Q000032:analysis; D009124:Muscle Proteins / Q000032:analysis; D009206:Myocardium / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D051381:Rats
D009124:Muscle Proteins; D011506:Proteins