title: Identification of an apparent aminoacyl-tRNA synthetase activator factor as tRNA nucleotidyltransferase.
abstract: The ability of yeast extracts to aminoacylate crude yeast tRNA with leucine and other amino acids is largely lost after chromatography of the extracts in DEAE-Sephadex. The original aminoacylating ability is restored by combining protein fractions from the DEAE-chromatogram. The characteristics of this reactivation are very similar to the activation, by protein factors, of certain aminoacyl-tRNA synthetases reported by others. The results in this work indicate that the apparent aminoacyl-tRNA synthetase activator factor is the tRNA nucleotidyltransferase and that the restoration of the original tRNA aminoacylating ability is a consequence of the repairing of the 3' end of incomplete tRNA chains.
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D004789:Enzyme Activation; D007935:Leucine-tRNA Ligase / Q000378:metabolism; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*; D012331:RNA, Fungal / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D012440:Saccharomyces / Q000201:enzymology
chemical_list: D012331:RNA, Fungal; D012343:RNA, Transfer; D012316:RNA Nucleotidyltransferases; C023198:tRNA nucleotidyltransferase; D000604:Amino Acyl-tRNA Synthetases; D007935:Leucine-tRNA Ligase