Antitumor activity of 2-keto-3-deoxyoctonate-free lipopolysaccharide of Vibrio anguillarum in mice.
The antitumor activity of a marine bacterium, Vibrio anguillarum, against Ehrlich carcinoma cells in ddY mice was investigated. The aqueous layer obtained by the hot phenol-water procedure exhibited more antitumor activity than did the middle layer or the phenol layer. This finding indicates that lipopolysaccharide (LPS) derived from V. anguillarum exhibits significant antitumor activity. In fact, mice injected with LPS obtained by ultracentrifugation and treatment with RNase had a longer mean survival period than the control mice. V. anguillarum LPS also inhibited the growth of syngeneic fibrosarcoma induced  by 3-methylcholanthrene in C57BL/6 mice. V. anguillarum LPS possesses no 2-keto-3-deoxyoctonate, a regular sugar component of the core region of most gram-negative bacterial LPS, suggesting that 2-keto-3-deoxyoctonate is unnecessary for the antitumor activity of LPS.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000628:therapy*; D005354:Fibrosarcoma / Q000628:therapy; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D011135:Polysaccharides, Bacterial / Q000627:therapeutic use*; D012513:Sarcoma, Experimental / Q000628:therapy; D014733:Vibrio / Q000276:immunology*
D011135:Polysaccharides, Bacterial