Characterization of pinocytic vesicles from CHO cells: resolution of pinosomes from lysosomes by analytical centrifugation.
Pinocytic vesicles (pinosomes) and lysosomes from suspension cultured, Chinese hamster ovary (CHO-S) cells have been resolved as two non-overlapping organelle populations by analytical centrifugation in Percoll gradients. Pinosomes were labeled with either horseradish peroxidase (HRP), a fluid phase content marker, or by radioiodination by pinocytosed lactoperoxidase (LPO). CHO-S cell lysosomes followed by three different marker enzymes and electron microscopy behaved as a single, dense organelle population. Pinosomes were partially resolved from plasma membrane, a less dense organelle population.
D000818:Animals; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D008247:Lysosomes / Q000648:ultrastructure*; D008854:Microscopy, Electron; D010053:Ovary / Q000166:cytology*; D010873:Pinocytosis