Uptake of triiodothyronine into cultured human muscle cells.
The uptake of T3 was measured in cultured human muscle cells at 37 degrees C and pH 7.4 in a medium containing albumin and glucose. The initial up]take increased linearly when the total T3 concentration was varied from 10(-9) to 10(-4) M. At prolonged incubation time the uptake decreased to virtually zero in about 30 min. These data indicate a rapid passive transport mechanism of T3 and a fast equilibration of the cellular T3 with the surrounding medium. In agreement with these conclusions the efflux of T3 was rapid and the initial uptake was not altered by pre-incubation in a T3-containing medium.
D001692:Biological Transport; D002455:Cell Division; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000378:metabolism; D003470:Culture Media; D004247:DNA / Q000378:metabolism; D006801:Humans; D009124:Muscle Proteins / Q000378:metabolism; D009132:Muscles / Q000166:cytology / Q000378:metabolism*; D014284:Triiodothyronine / Q000378:metabolism*
D003470:Culture Media; D009124:Muscle Proteins; D014284:Triiodothyronine; D004247:DNA; D003402:Creatine Kinase