title: Energetics of smooth muscle taenia caecum of guinea-pig: a 31P-NMR study.
abstract: Smooth muscle cell energetics of taenia caeci during relaxation, spontaneous activity and maximal contraction were investigated using 31P-NMR. In relaxed muscle obtained in calcium-free medium, [ATP], [phosphocreatine] and [sugar phosphate] were 4.4 mM, 7.7 mM and 2.8 mM, respectively. There was only a small difference in the energetics of spontaneously active and maximally contracted muscles, but under both conditions substantial changes occurred as compared with relaxed muscles. The internal pH in relaxed muscle was found to be 7.05, which acidified to 6.5 during contraction. The level of sugar phosphates was found to be not a limiting factor in energetics.
mesh_terms: D000818:Animals; D002432:Cecum / Q000378:metabolism; D004734:Energy Metabolism; D005260:Female; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000378:metabolism* / Q000502:physiology; D010710:Phosphates / Q000378:metabolism
chemical_list: D010710:Phosphates