Lipogenesis in primary cultures of adipoblasts derived from genetically obese Zucker rats.
Adipocyte precursor cultures prepared from the epididymal fat pads of genetically obese (fa/fa) and lean (Fa/Fa) Zucker rats grow similarly in culture. Addition of enriched medium (EM) containing human serum, insulin, and glucose stimulated lipid filling of the adipocyte precursors in both cultures. However, [3H] H2O incorporation into total lipids, fatty acid synthetase and lipoprotein lipase activities, and cytosolic protein contents are all decreased in the fa/fa compared with the Fa/Fa cultures. Substitution of lean or obese rat serum for human serum in the enriched medium does not alter the decreased lipogenic capacity of the fa/fa adipocyte precursor cultures.
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D064429:Fatty Acid Synthases / Q000378:metabolism; D005838:Genotype; D008055:Lipids / Q000096:biosynthesis*; D008071:Lipoprotein Lipase / Q000378:metabolism; D008297:Male; D009765:Obesity / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011924:Rats, Zucker
D008055:Lipids; D064429:Fatty Acid Synthases; D008071:Lipoprotein Lipase