Monoclonal antibodies can discriminate between some active and inactive forms of leukocyte interferon.
Antiviral activity of recombinant human leukocyte A interferon was inactivated by heating at 65 degrees C or by reduction of disulfide bonds. The specific immunoreactivity, as measured by radioimmunoassays measuring binding to monoclonal antibodies, decreased concomitantly with the antiviral activity. Although the monoclonal antibodies did bind to inactivated interferon, their binding affinity to inactivated interferon was in general very much lower than their binding affinity to active interferon. Therefore, this immunoassay could replace the antiviral assay for detection of biologically active interferon. In addition, most of these antibodies should be especially useful for purification of the interferons since they discriminate between the native active and inactive denatured species. Screening for such antibodies is convenient and simple. The general use of antibodies that preferentially interact with native molecules provides a powerful new principle for choosing monoclonal antibodies with extraordinary potential in assay and purification.
D000911:Antibodies, Monoclonal; D001681:Biological Assay; D006358:Hot Temperature; D006801:Humans; D007120:Immunochemistry; D007370:Interferon Type I / Q000302:isolation & purification*; D011489:Protein Denaturation; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D007370:Interferon Type I