Rapid, efficient cloning of murine hybridoma cells in low gelation temperature agarose.
A rapid, efficient method is described for cloning of lymphocyte hybridoma cells. The method utilizes ultra-low gelation temperature agarose.
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002999:Clone Cells; D046508:Culture Techniques / Q000379:methods; D005260:Female; D006825:Hybridomas / Q000276:immunology*; D007118:Immunoassay; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D010954:Plasmacytoma / Q000276:immunology; D012685:Sepharose; D013696:Temperature
D000911:Antibodies, Monoclonal; D012685:Sepharose