Analysis of total urinary catecholamines by liquid chromatography: methodology, routine experience and clinical interpretations of results.
A simple routine method is described for simultaneous assay of total urinary adrenaline, noradrenaline and dopamine. The catecholamines are pre-purified on a small ion-exchange column, separated by reversed phase ion-pair liquid chromatography, and are quantitated by electrochemical detection. The method was routinely applied to 422 urines. Elevated values were found in four urine specimens obtained from patients with histologically proven phaeochromocytomas. Virtually no interference by endogenous or exogenous compounds was found. Values for urinary catecholamines determined by fluorimetric analysis agreed with those obtained by high pressure liquid chromatography with electrochemical detection. Within-day CVs for the compounds ranged from 5.2-11.9%, between-day CVs from 3.3-6.6%. The normal range (95% confidence level) was 20-230 micrograms/24 h for noradrenaline and 1-35 micrograms/24 h for adrenaline.
D002395:Catecholamines / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004298:Dopamine / Q000652:urine; D004837:Epinephrine / Q000652:urine; D006801:Humans; D009638:Norepinephrine / Q000652:urine; D010673:Pheochromocytoma / Q000652:urine; D013050:Spectrometry, Fluorescence
D002395:Catecholamines; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine