A fluorimetric method for red blood cell sorbitol dehydrogenase activity.
A new fluorimetric method for the quantification of red blood cell (RBC) sorbitol dehydrogenase is described. It is based on the oxidation of sorbitol to fructose, in presence of NAD+, catalysed by the RBC-sorbitol dehydrogenase. The quantity of NADH formed is then measured in a filter fluorimeter. Comparison with an indirect spectrophotometric assay yielded good correlation; however, the present method offers several advantages: it is more rapid, simple and inexpensive. It should be useful to screen for sorbitol dehydrogenase deficiency in large numbers of individuals, particularly patients with diabetes or cataracts.
D004912:Erythrocytes / Q000201:enzymology*; D005470:Fluorometry / Q000379:methods; D006801:Humans; D007700:Kinetics; D007064:L-Iditol 2-Dehydrogenase / Q000097:blood*; D013053:Spectrophotometry; D013401:Sugar Alcohol Dehydrogenases / Q000097:blood*
D013401:Sugar Alcohol Dehydrogenases; D007064:L-Iditol 2-Dehydrogenase