title: Specific estrogen binding sites in human lymphoid cells and thymic cells.
abstract: The binding of estrogen in preparations of human peripheral blood mononuclear cells, as well as by splenic and thymic cells is demonstrated by three different approaches (Dextran-coated charcoal method, whole cell assay, and gel filtration on a sepharose 4B column). Scatchard's analysis of [3H]-moxestrol (R2858) and [3H]-estradiol binding proves the existence of a single class of receptor sites having a dissociation constant of 0.18-2.4 X 10(-9) M. Physicochemical properties of the binder, including binding capacity and steroid specificity, are quite similar to those reported for the thymus of small mammalian species or human thymoma.
mesh_terms: D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism*; D013950:Thymus Gland / Q000378:metabolism*
chemical_list: D011960:Receptors, Estrogen