title: The effect of vitamin C on in vivo lipid peroxidation in guinea pigs as measured by pentane and ethane production.
abstract: Measurements of pentane and ethane as indices of in vivo lipid peroxidation were made on samples of breath from vitamin C-sufficient and vitamin C-deficient guinea pigs injected with 23 microliters carbon tetrachloride (CCl4)/100 g body wt. Vitamin C-deficient animals produced significantly more pentane and ethane after CCl4 treatment than did vitamin C-sufficient guinea pigs. Pretreatment of vitamin C-deficient animals with 75 mg ascorbic acid/100 g body wt significantly lowered both pentane and ethane evolution. Protection against in vivo lipid peroxidation similar to that provided by ascorbic acid was also found when vitamin C-deficient guinea pigs were pretreated with isoascorbic acid, reduced glutathione, alpha-tocopherol or beta-carotene. When animals were pretreated with the radical scavenger mannitol, a protective effect was also observed as measured by pentane evolution.
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D001206:Ascorbic Acid Deficiency / Q000378:metabolism; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism; D004980:Ethane / Q000378:metabolism*; D006168:Guinea Pigs; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D010420:Pentanes / Q000378:metabolism*; D013997:Time Factors
chemical_list: D008054:Lipid Peroxides; D010420:Pentanes; C033353:pentane; D004980:Ethane; D001205:Ascorbic Acid