title: Acylation of lysoglycerophospholipids by adrenal membranes.
abstract: 1. Substantial differences were found in the acyl donor and lyso-acceptor specificities among subcellular membranes and with respect to different regions of the adrenal gland. 2. In the presence of Mg2+-ATP and CoASH, adrenal microsomes were actively transferring arachidonate to lysophospholipids with acyl acceptor specificity in the order: 1-acyl-GPI greater than 1-acyl-GPC greater than 1-acyl-GP. However, when oleoyl-CoA was used, acyl acceptor specificity for the microsomal transferases was in the order: 1-acyl-GPC greater than 1-acyl-GP greater than 1-acyl-GPI. 3. Mitochondrial membranes had very low acyl transfer activity and they preferred 1-acyl-GPC over other lyso-acceptors. 4. The chromaffin granules were apparently lacking this type of activity.
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism; D000215:Acylation; D000217:Acyltransferases / Q000378:metabolism*; D000311:Adrenal Glands / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D002417:Cattle; D005994:Glycerophosphates / Q000378:metabolism*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism; D008297:Male; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D000214:Acyl Coenzyme A; D001095:Arachidonic Acids; D005994:Glycerophosphates; C017585:oleoyl-coenzyme A; D000217:Acyltransferases