Binding of terbium to apoferritin: a fluorescence study.
Luminescence measurements show that apoferritin binds three Tb(III) atoms per subunit in accordance with crystallographic evidence. Fe(II) competes with Tb(III) for at least some of the binding sites. This competition may be the molecular basis for the inhibition of iron incorporation into apoferritin brought about by Tb(III). Ca(II), which is generally replaced by Tb(III) in Ca(II) binding proteins, does not compete with the lanthanide for binding to apoferritin.
D000818:Animals; D001052:Apoferritins / Q000378:metabolism*; D001665:Binding Sites; D001667:Binding, Competitive; D005293:Ferritins / Q000031:analogs & derivatives*; D006736:Horses; D007501:Iron / Q000378:metabolism; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D013154:Spleen / Q000378:metabolism; D013725:Terbium / Q000378:metabolism*
D013725:Terbium; D005293:Ferritins; D001052:Apoferritins; D007501:Iron