[Use of disc electrophoresis in a linear gradient of polyacrylamide gel concentrations for simultaneous fractionation of total serum proteins, haptoglobins and lipoproteins].
Conditions are developed for fractionation of total proteins of blood serum and lipoproteids within a linear gradient of the polyacrylamide gel concentration. Optimal separation of the proteins was observed at initial concentrations of acrylamide 3 and 10%: lipoproteids--2 and 15%. The composition of haptoglobins should be taken into consideration when analyzing the spectrum of human serum proteins. For this purpose samples fractionated simultaneously in one gel plate should be subjected to differential staining with amide black and benzidine. It is established that the efficiency of alpha-lipoproteids subfractions separation is reached by electrophoresis of this class lipoproteids after polyethylene glycol precipitation. The method described broadens essentially potentialities of electrophoretic fractionation of blood serum proteins due to an increase in the resolving power and sensitivity.
D000328:Adult; D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D004589:Electrophoresis, Disc / Q000379:methods; D006242:Haptoglobins / Q000032:analysis*; D006801:Humans; D008074:Lipoproteins / Q000032:analysis* / Q000097:blood; D008875:Middle Aged; D051381:Rats
D001798:Blood Proteins; D006242:Haptoglobins; D008074:Lipoproteins
Primenenie disk-elektroforeza v lineinom gradiente kontsentratsii poliakrilamidnogo gelia dlia odnovremennogo fraktsionirovaniia summarnykh belkov syvorotki krovi, gaptoglobinov i lipoproteidov.