Changes in the binding of radioactive conjugated bile salts to serum proteins in cholestatic jaundice.
1. A micro-partition centrifugal ultrafiltration technique has been used to monitor the percentage of [14C]glycocholate, [3H]glycochenodeoxycholate and [3H]glycochenodeoxycholate-3-sulphate bound to serum proteins of patients with cholestatic liver disease. 2. In comparison with normal individuals the percentage of binding of [14C]glycocholate and, to a lesser extent, of [3H]glycochenodeoxycholate and [3H]glycochenodeoxycholate-3-sulphate was reduced. 3. The binding of [14C]glycocholate was inversely related to the serum bile salt and bilirubin concentrations. In contrast, the binding of [3H]glycochenodeoxycholate and [3H]glycochenodeoxycholate-3-sulphate were not altered by the severity of the cholestasis. 4. Studies in vitro indicated that the reduction in the binding of [14C]glycocholate in cholestatic liver disease was not due to high concentrations of bile salts, unconjugated bilirubin or fatty acids.
D001647:Bile Acids and Salts / Q000097:blood*; D001663:Bilirubin / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism*; D002779:Cholestasis / Q000097:blood*; D005999:Glycochenodeoxycholic Acid / Q000031:analogs & derivatives / Q000097:blood; D006000:Glycocholic Acid / Q000097:blood; D006801:Humans; D011485:Protein Binding
D001647:Bile Acids and Salts; D001798:Blood Proteins; D005999:Glycochenodeoxycholic Acid; C038329:glycochenodeoxycholate-3-sulfate; D006000:Glycocholic Acid; D001663:Bilirubin