Isotachophoretic pattern of LDH inhibiting fractions obtained from uremic ultrafiltrate by gelchromatography.
Uremic ultrafiltrate was fractioned by chromatography on Sephadex G15; two fractions were highly inhibiting the total lactate dehydrogenase activity in rat kidney homogenate. The inhibiting fractions were eluates number 10 and 11 with an elution volume of respectively 17.4 ml and 19.3 ml and a Kav of 0.17 and 0.25. Both fractions presented a different pattern on analysis by analytical isotachophoresis.
D000818:Animals; D002850:Chromatography, Gel; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors*; D008297:Male; D008875:Middle Aged; D051381:Rats; D011919:Rats, Inbred Strains; D014462:Ultrafiltration; D014511:Uremia / Q000097:blood* / Q000201:enzymology
D007770:L-Lactate Dehydrogenase