Discrimination between macrophage-and NK-type tumoricidal activities via anti-asialo GM1 antibody.
The usefulness of asialo GM1, a glycolipid surface marker, to define the effector cell types involved in tumor resistance in vitro and in vivo was assessed. Pretreatment of rat effector cells with anti-asialo GM1 antibody plus complement in vitro either abrogated or markedly diminished NK activity; in contrast, macrophage-type cytocidal activity was not diminished by such pretreatment. Similarly, systemic inoculation of anti-asialo GM1 antibody selectively eliminated NK activity, leaving macrophage-type tumoricidal reactivity intact. Finally, such pretreatment did not diminish host resistance in an in vivo tumor model in which the available evidence suggests a critical role for macrophages. The asialo GM1 marker may thus be useful in delimitating the tumoricidal capacity of cells exhibiting NK activity from that mediated by other cell types.
D000818:Animals; D000906:Antibodies; D003602:Cytotoxicity, Immunologic; D005354:Fibrosarcoma / Q000276:immunology; D005677:G(M1) Ganglioside; D006028:Glycosphingolipids / Q000032:analysis / Q000276:immunology*; D007694:Killer Cells, Natural / Q000032:analysis / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology; D008264:Macrophages / Q000032:analysis / Q000276:immunology*; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology*; D051381:Rats
D000906:Antibodies; D006028:Glycosphingolipids; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside