[Kinetics of sulphur compounds decrease in liver homogenates during in vitro incubation in experimental conditions modifying lipid peroxidation rate].
Low values of pH are known to increase lipid peroxidation during "in vitro" incubation of rat liver homogenates. Non protein sulphydryl compounds decrease more rapidly when the pH of the homogenate is lower. The increase in incubation temperature stimulates the production of TBArs. At high temperature values the -SH groups content of liver tissue falls quickly; the rate of this fall, at the same temperature values, shows differences at different pH. In all experimental conditions the decrease of -SH groups precedes the stimulation of TBArs production and it is mainly due to GSH oxidation, being the decrement of total glutathione very low. Total homogenate from Yoshida AH-130 hepatoma shows no production of GSSG even when incubate for 2 hours at 40 degrees C and at pH 6. The importance of the role played by carbonyl compounds arising from peroxidative decomposition of unsaturated fatty acids on GSH decrement in liver homogenates is discussed and the difference between Yoshida hepatoma and normal liver, as far as GSH decrease is concerned, is discussed.
D000818:Animals; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008054:Lipid Peroxides / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013696:Temperature
D008054:Lipid Peroxides; D013438:Sulfhydryl Compounds
[Kinetics of sulphĭdryl compounds decrease inliver homogenates during "in vitro" incubation in experimental conditions modifying lipid peroxidation rate. Comparison with the kinetics of -SH groups in homogenates from Yoshida AH-130 hepatoma]