Insulin receptor: evidence that it is a protein kinase.
Highly purified preparations of insulin receptor catalyzed the phosphorylation of the 95,000-dalton subunit of the insulin receptor. This subunit of the insulin receptor was also labeled with [alpha-32P]8-azidoadenosine 5'-triphosphate, a photoaffinity label for adenosine triphosphate binding sites. The identity of the 95,000-dalton band was confirmed in both cases by precipitation with a monoclonal antibody to the insulin receptor. These results suggest that the insulin receptor is itself a protein kinase.
D000255:Adenosine Triphosphate / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D008214:Lymphocytes; D008970:Molecular Weight; D010750:Phosphoproteins / Q000502:physiology; D011494:Protein Kinases / Q000502:physiology*; D011972:Receptor, Insulin / Q000502:physiology*
D010750:Phosphoproteins; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D011972:Receptor, Insulin