A non-surgical technique for the transcervical administration of physiological and pharmacological agents into rat uteri.
A technique for the instillation of solutions into the rat uterine lumen is described. The method has been tested by following the receptor depletion/replenishment cycle after oestradiol injection and by checking on the stoichiometry of hormone/receptor translocation from the cytoplasmic compartment into the nucleus. Both crude sediments from frozen uteri and nuclei purified by a novel procedure were analyzed and gave identical results. The limitations and the advantages of the technique are discussed.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D003593:Cytoplasm / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004958:Estradiol / Q000008:administration & dosage / Q000378:metabolism; D005260:Female; D007267:Injections / Q000379:methods*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D011959:Receptors, Estradiol; D011960:Receptors, Estrogen / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism* / Q000648:ultrastructure
D011959:Receptors, Estradiol; D011960:Receptors, Estrogen; D004958:Estradiol