Increased humoral and cellular immunity in mice inoculated with allogeneic tumor cells attached to microcarrier beads.
A murine carcinoma cell line was grown in a microcarrier culture and was used for immunization of allogeneic mice. It was found that inoculation of cells attached to microcarrier beads resulted in heightened serum titers of cytotoxic antibodies and in a stronger cell-mediated cytotoxic reactivity in the spleen compared to cells detached from the substrate. It is proposed that immunization of animals with anchorage-dependent cells should be carried out while the cells are still adherent to the culture microcarriers.
D000818:Animals; D000912:Antibodies, Neoplasm / Q000096:biosynthesis; D000917:Antibody Formation; D002448:Cell Adhesion; D003602:Cytotoxicity, Immunologic; D005260:Female; D007111:Immunity, Cellular; D008175:Lung Neoplasms / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008863:Microspheres; D009368:Neoplasm Transplantation; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000912:Antibodies, Neoplasm