title: Activation of fibroblasts induced by mononuclear cells from patients with systemic scleroderma and inactivation by mitogen stimulation.
abstract: Unstimulated mononuclear cell supernatants from patients with systemic scleroderma significantly augmented the DNA and collagen biosynthesis of 3T3 cells compared to normal mononuclear cells. This increase was suppressed by either a particular glycosaminoglycan, which from previous studies, has appeared to act as one of the tissue antigens in sclerotic skin, or concanavalin A stimulation.
mesh_terms: D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D003208:Concanavalin A / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D008875:Middle Aged; D008934:Mitogens / Q000378:metabolism*; D012595:Scleroderma, Systemic / Q000378:metabolism*
chemical_list: D006025:Glycosaminoglycans; D008934:Mitogens; D003208:Concanavalin A; D003094:Collagen; D004247:DNA