[Isolation of skeletal muscle plasmatic membranes as closed vesicles].
Ultrasonic treatment of skeletal muscle sarcolemma preparations in 0.25 M sucrose, 0.1 M NaCl, 0.001 M tris-HCl-buffer (pH 7.4) induces formation of closed osmotically active vesicles which may be isolated by centrifugation of a sonicated suspension within the sucrose density gradient. The vesicles retain the enzymic activity peculiar to the initial sarcolemma preparations.
D000818:Animals; D002499:Centrifugation, Density Gradient; D007425:Intracellular Membranes / Q000032:analysis*; D008081:Liposomes / Q000302:isolation & purification*; D008854:Microscopy, Electron; D009132:Muscles / Q000032:analysis*; D011817:Rabbits; D012508:Sarcolemma / Q000032:analysis* / Q000201:enzymology / Q000648:ultrastructure; D013010:Sonication; D013053:Spectrophotometry
D008081:Liposomes
Vydelenie plazmaticheskikh membran skeletnykh myshts v vide zamknutykh vezikul.