Spectroscopic evidence for the formation of a transient species during cytochrome P-450scc induced hydroperoxysterol-glycol conversions.
Spectroscopic analysis of the interaction of the epimeric 20-hydroperoxy derivatives of cholesterol with bovine adrenocortical cytochrome P-450scc preparations suggested the formation of a transient species. The intermediate was detected at 4 degrees C and characterized by a minimum at 412 nm in the difference spectrum.
D000302:Adrenal Cortex / Q000201:enzymology; D000818:Animals; D002384:Catalysis; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D006018:Glycols / Q000378:metabolism*; D013053:Spectrophotometry; D013237:Stereoisomerism
D006018:Glycols; C038027:20-hydroperoxycholesterol; D003577:Cytochrome P-450 Enzyme System; D002784:Cholesterol