Vitamin K-dependent carboxylase: synthesis of an inhibitor of the glutamyl binding site.
Liver microsomes contain a vitamin K and O2-dependent carboxylase that converts peptide-bound glutamyl residues to gamma-carboxyglutamate residues. The peptide Boc-O-phospho-Ser-O-phospho-Ser-Leu-OMe has now been synthesized. This peptide inhibits the carboxylation of endogenous protein precursors by a detergent-solubilized preparation of the carboxylase and is an apparent competitive inhibitor of the carboxylation of Phe-Leu-Glu-Glu-Leu.
D015055:1-Carboxyglutamic Acid / Q000096:biosynthesis; D000595:Amino Acid Sequence; D000818:Animals; D019735:Carbon-Carbon Ligases; D007700:Kinetics; D008025:Ligases / Q000037:antagonists & inhibitors*; D008862:Microsomes, Liver / Q000201:enzymology*; D009842:Oligopeptides / Q000138:chemical synthesis; D010446:Peptide Fragments / Q000494:pharmacology; D051381:Rats; D013329:Structure-Activity Relationship; D014812:Vitamin K / Q000378:metabolism*
D009842:Oligopeptides; D010446:Peptide Fragments; D014812:Vitamin K; D015055:1-Carboxyglutamic Acid; D008025:Ligases; D019735:Carbon-Carbon Ligases; C020660:glutamyl carboxylase