Measurement of immunoreactive alpha-melanocyte-stimulating hormone in the blood of rainbow trout kept under various conditions.
Initial attempts to measure alpha-MSH in trout blood by radioimmunoassay proved unsuccessful due to apparent losses of immunoreactive hormone, especially in the plasma of trout adapted to a black background. An extraction protocol for alpha-MSH from plasma is described. Subsequent radioimmunoassay revealed progressive increases in immunoreactive alpha-MSH as trout became adapted to a black background. Plasma alpha-MSH titres were raised during fungal infection, and also showed significant differences in fish obtained from different fish farms.
D000818:Animals; D005393:Fish Diseases / Q000097:blood; D005667:Furunculosis / Q000097:blood; D009074:Melanocyte-Stimulating Hormones / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods; D012487:Salmonidae / Q000097:blood*; D012880:Skin Pigmentation; D014337:Trout / Q000097:blood*
D009074:Melanocyte-Stimulating Hormones