Affinity chromatography of acyl-CoA utilizing enzymes on Procion Red-agarose.
The dye Procion Red HE3B immobilized on agarose and available as Matrex Gel Red A is shown to bind citrate synthase and succinate thiokinase from a number of diverse organisms. Salt-gradient elution removes the enzymes in high yields and with substantial purification. The elution profiles follow a pattern similar to that of the molecular size variations of the enzymes.
D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity; D002950:Citrate (si)-Synthase / Q000032:analysis*; D003066:Coenzyme A Ligases / Q000032:analysis*; D007652:Oxo-Acid-Lyases / Q000032:analysis*; D012685:Sepharose; D013389:Succinate-CoA Ligases / Q000032:analysis*; D014227:Triazines
D014227:Triazines; C020795:procion red HE-3B; D012685:Sepharose; D002950:Citrate (si)-Synthase; D007652:Oxo-Acid-Lyases; D003066:Coenzyme A Ligases; D013389:Succinate-CoA Ligases