Development of a homologous radioimmunoassay for secreted hamster prolactin.
A specific and sensitive homologous radioimmunoassay has been developed for secreted hamster prolactin. Hamster serum and pituitary extracts showed parallel dilution-response curves with hamster prolactin. The sensitivity of the assay ranged from 0.5 to 1.0 ng/ml, and the intra- and inter-assay coefficients of variation were 6 and 10%, respectively. Additionally we have demonstrated that the rat prolactin radioimmunoassay kit distributed by the National Institute of Arthritis, Metabolism, and Digestive Diseases is an inadequate method for the measurement of hamster prolactin.
D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology; D006224:Cricetinae / Q000276:immunology*; D005260:Female; D007106:Immune Sera / Q000276:immunology; D007774:Lactation; D010902:Pituitary Gland / Q000032:analysis; D011247:Pregnancy; D011388:Prolactin / Q000032:analysis* / Q000097:blood / Q000276:immunology; D011863:Radioimmunoassay / Q000592:standards*; D051381:Rats; D011933:Reagent Kits, Diagnostic / Q000592:standards; D013045:Species Specificity
D007106:Immune Sera; D011933:Reagent Kits, Diagnostic; D001971:Bromocriptine; D011388:Prolactin