Simultaneous determination of the concentration and lytic activity of effector cells that mediate natural and antibody-dependent cytotoxicity.
Cellular cytotoxicity reactions can be studied in a manner analogous to that used to measure enzyme activity. This approach yields two parameters: Vmax, the maximal rate of target cell lysis that can be achieved by the lymphocyte preparation tested, and KMapp, the apparent Michaelis constant. By analogy to many enzyme-catalysed reactions, KMapp values for cytotoxicity reactions have generally been interpreted in terms of dissociation constants for the interaction of receptor sites on effector cells with antigens on the target cells. In this paper we demonstrate that experimentally determined KMapp values for natural or antibody-dependent cytotoxicity reactions are approximately equal to the concentration of NK or K effector cells in the lymphocyte preparation tested. This result makes possible the simultaneous determination of both effector cell frequency and lytic activity in a given lymphocyte preparation.
D000328:Adult; D000920:Antibody-Dependent Cell Cytotoxicity; D002452:Cell Count; D002460:Cell Line; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D007700:Kinetics