Kinetics of Lucifer yellow CH efflux in giant mitochondria.
The fluorescent dye Lucifer yellow CH was microinjected electrophoretically into giant mitochondria isolated from mice maintained on a diet containing cuprizone. The dye was retained by the mitochondria, indicating that it was contained in a space bounded by a selectively permeable membrane. The labelling was reversible by reversing the polarity of the current. A study of the disappearance of the fluorescence indicates that the permeability of the mitochondrial membrane to the dye (probably the lithium and/or the potassium salts) ranges from 10(-7) to 10(-8) cm/s.
D000818:Animals; D002458:Cell Fractionation; D003471:Cuprizone / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000378:metabolism*; D007546:Isoquinolines / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D008856:Microscopy, Fluorescence; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
D005456:Fluorescent Dyes; D007546:Isoquinolines; D003471:Cuprizone; C017475:lucifer yellow