title: The accumulation of the maternal pool of histone H1A during oogenesis in Xenopus laevis.
abstract: The accumulation of the maternal pool of histone H1A in Xenopus laevis oocytes was measured by the use of a semi-quantitative immunoradiographic method. This method implies the size-fractionation of total basic protein extracts from oocytes on a polyacrylamide gel, blotting of the proteins to diazotized paper and detection of histone proteins on the blots with the aid of antibodies to histone H1A and of [125I]protein A. It is shown that the maternal H1A pool that is present in fully-grown oocytes is accumulated very early in oogenesis, mainly in early vitellogenic oocytes. Stage III oocytes (Dumont, 1972) already contain approximately 80% of the final amount of H1A that is found in mature oocytes. The minimum amount of histone H1A estimated to be present in mature oocytes is 8-10 ng.
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006657:Histones / Q000378:metabolism*; D009865:Oocytes / Q000032:analysis; D009866:Oogenesis; D011506:Proteins / Q000032:analysis; D014982:Xenopus laevis / Q000378:metabolism*
chemical_list: D006657:Histones; D011506:Proteins