Effect of human colonic microsomes and cell-free extracts of Bacteroides fragilis on the mutagenicity of 2-aminoanthracene.
It has been previously demonstrated that whereas the microsomal fraction of rat colonic tissue and cell-free extract of Bacteroides fragilis alone do not convert 2-aminoanthracene into a mutagen, both together have this capacity in a model using the Ames technique. The aim of the present study was to extend these observations to human colonic tissue and to compare colonic mucosa with other tissues. It was found that whereas colonic microsomal fraction and cell-free extract of Bacteroides fragilis alone possessed little activating capacity with regard to 2-aminoanthracene, both together activated 2-aminoanthracene into a mutagen in this model. The microsomal fraction of other tissues, such as kidney, brain, muscle, and gastric and ileal mucosae, possessed activities similar to colonic mucosa.
D000873:Anthracenes / Q000494:pharmacology*; D001441:Bacteroides fragilis; D003106:Colon / Q000648:ultrastructure*; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000648:ultrastructure; D008861:Microsomes / Q000378:metabolism*; D009152:Mutagenicity Tests; D009153:Mutagens
D000873:Anthracenes; D009153:Mutagens; C006593:2-anthramine