The affinity of metergoline for 3H-serotonin (5HT) binding sites is regulated by guanine nucleotide.
100 microM guanine nucleotide Gpp (NH)p reduces the affinity of the serotonergic antagonist metergoline for 3H-5HT binding sites in rat cerebral cortex. This effect is present both in inhibition binding and in saturation experiments. The hypothesis that the interaction of some serotonergic antagonists with 3H-5HT binding sites is regulated by guanine nucleotides is discussed.
D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D002540:Cerebral Cortex / Q000378:metabolism*; D004873:Ergolines / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives*; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology*; D008297:Male; D008711:Metergoline / Q000378:metabolism*; D051381:Rats; D011985:Receptors, Serotonin / Q000187:drug effects / Q000378:metabolism*
D004873:Ergolines; D011985:Receptors, Serotonin; D008711:Metergoline; D006165:Guanylyl Imidodiphosphate; D006160:Guanosine Triphosphate