Corneal transparency changes resulting from osmotic stress.
Corneal thickness and transparency changes were measured following exposure of the cornea to various levels of osmotic stress. Bathing the cornea in hypotonic solutions of NaCl caused an increase in corneal thickness and light scatter within the cornea. Transparency changes with hypotonicity were determined by comparing the relative brightness of haloes around a bright lamp source for the various concentrations of NaCl solutions. Corresponding corneal thickness changes were determined by pachometry. A nearly linear relationship was found between solution hypotonicity and the degree of corneal swelling, whilst an exponential relationship was found between hypotonicity and relative halo brightness. The rate of corneal recovery from osmotic stress was found to be constant with different degrees of tonicity, although the rate of thickness recovery was much slower than the recovery from haloes.
D003315:Cornea / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007038:Hypotonic Solutions; D008297:Male; D009997:Osmotic Pressure; D012965:Sodium Chloride / Q000494:pharmacology*; D013997:Time Factors
D007038:Hypotonic Solutions; D012965:Sodium Chloride