The synthesis and metabolism of [6-3H]-25-hydroxycholesterol in rat adrenal tumor cells.
The synthesis of [6-3H]-25-hydroxycholesterol from 26-norcholest-5-en-3 beta-ol-25-one is described. The metabolism of the radiolabeled hydroxysterol in rat adrenocortical carcinoma cells and in mitochondria-enriched preparations of rat adrenal tissue was investigated. We found that [6-3H]-25-hydroxycholesterol was metabolized efficiently to [3H]-pregnenolone in both preparations of rat adrenal tissue.
D000306:Adrenal Cortex Neoplasms / Q000378:metabolism*; D000311:Adrenal Glands / Q000378:metabolism; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D006888:Hydroxycholesterols / Q000138:chemical synthesis / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000378:metabolism; D011284:Pregnenolone / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D006888:Hydroxycholesterols; D011284:Pregnenolone; C007997:25-hydroxycholesterol