Hydrolysis of the biliary glucuronic acid conjugate of phenol by the intestinal mucus/flora of goldfish (Carassius auratus).
1. An investigation was carried out on the hydrolytic activity of the intestinal mucus/flora on phenylglucuronide in the bile of goldfish. 2. Approximately 79% of the total phenylglucuronide (3.4 mumol) in the bile was hydrolysed after 16 h incubation with the intestinal mucus/flora. 3. Of the total phenol in the aquarium water of goldfish exposed previously to phenol for 48 h, 41% was found to be phenylglucuronide when fish were placed in a phenol-free medium and were dosed hourly for 8 h with D-saccharic acid 1,4-lactone to inhibit beta-glucuronidase activity in the intestine.
D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D003530:Cyprinidae / Q000378:metabolism*; D005965:Glucuronates / Q000378:metabolism*; D006054:Goldfish / Q000378:metabolism*; D006868:Hydrolysis; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000382:microbiology
D005965:Glucuronates; C025298:phenylglucuronide