Use of 5-BrdUrd/313 nm light technique for identification of cells in initial S-phase within GM-CFCc compartment.
Long-term, low-level, BrdUrd infusion identifies two subpopulations of GM-CFCc with quite dissimilar sensitivities to 313 nm light. The responses of these two GM-CFCc subpopulations to hydroxyurea indicate that both are rapidly proliferating at the time of the assay. However, the absolute UV-light sensitivity of the S-phase components and the effects of increasing BrdUrd concentration indicate that the two GM-CFCc subpopulations passed through the previous cell cycle at widely disparate rates. Further, those GM-CFCc originating from a parental cell with a slow turnover are associated with a lower buoyant density than those GM-CFCc that have been in rapid cycle for at least two generations. These results indicate that the resistance to 313 nm-light irradiation, shown by S-phase cells in the first cell cycle of the BrdUrd labeling, may provide evidence of the proliferative history of the cell being assayed.
D000818:Animals; D001972:Bromodeoxycytidine / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002453:Cell Cycle; D002499:Centrifugation, Density Gradient; D003114:Colony-Forming Units Assay / Q000145:classification*; D005260:Female; D006918:Hydroxyurea / Q000494:pharmacology; D007399:Interphase; D007700:Kinetics; D008027:Light; D051379:Mice
D001972:Bromodeoxycytidine; D001973:Bromodeoxyuridine; D006918:Hydroxyurea