Multiplicity of allergens in peanuts.
Crude peanut protein fractions from raw and roasted peanuts were examined in the RAST with 10 sera from patients showing clinical peanut sensitivity. The radioactive uptake results, which were generally high, did not reveal any distinguishable pattern. Two commercially available peanut proteins, peanut lectin and phospholipase D, gave poor RAST responses. Three purified peanut proteins, alpha-arachin, conarachin I, and concanavalin A-reactive glycoprotein, all gave significant RAST results that were generally lower than those obtained with the crude extracts. The extent of RAST inhibition obtained with these materials was inversely related to their abundance in the total peanut protein. Crossed immunoelectrophoresis with extracts from raw and roasted peanut indicated the presence of 22 and 10 anodically migrating antigens, respectively. Sixteen IgE binding antigens were revealed for raw peanut and seven for roasted peanut after incubation with a mixed serum from the 10 patients in crossed radioimmunoelectrophoresis (CRIE) using 125I-labeled anti-IgE. CRIE plates treated with individual serum samples showed that all the patients had specific IgE for the major antigen peak, which has been tentatively identified as alpha-arachin. This major storage protein of peanut, which is known to be particularly heat resistant; may be of greater clinical significance than its apparently low RAST activity would seem to indicate.
D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000032:analysis*; D052179:Antigens, Plant; D010367:Arachis / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003208:Concanavalin A / Q000276:immunology; D003378:Counterimmunoelectrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D037102:Lectins / Q000276:immunology; D008297:Male; D010739:Phospholipase D / Q000276:immunology; D037121:Plant Lectins; D010940:Plant Proteins / Q000276:immunology*; D011852:Radioallergosorbent Test; D012967:Sodium Dodecyl Sulfate
D000485:Allergens; D052179:Antigens, Plant; D006023:Glycoproteins; D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; C000502:conarachin I protein, peanut; D003208:Concanavalin A; C000102:Arachin; D012967:Sodium Dodecyl Sulfate; D007073:Immunoglobulin E; D010739:Phospholipase D