A high recovery method for concentrating microgram quantities of protein from large volumes of solution.
A method is presented for the recovery of 40-80% of the protein from a 1 microgram/ml solution. The final protein pellet is free of detergent and other ionic compounds and is thus compatible with any denaturing solution. The primary structure of the protein is unaffected by the procedure, making the final pellet an ideal sample for any analytical procedure to determine protein structure.
D011232:Chemical Precipitation; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D008832:Microchemistry; D011489:Protein Denaturation; D011506:Proteins / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate; D012996:Solutions
D003902:Detergents; D011506:Proteins; D012996:Solutions; D012967:Sodium Dodecyl Sulfate