Localized reactivation of the principal piece of demembranated hamster sperm by iontophoretic application of ATP.
Mature hamster sperm were demembranated with Triton X-100, and the principal piece of the flagellum was reactivated by iontophoretic application of ATP. The response was a brief local straightening of a short length of the flagellum followed by the formation of a pair of bends at the two ends of the straight region. The formation of a PR (principal and reverse bends in a proximo-distal sequence) or an RP bend-pair is interpreted as a result of active sliding of doublet subset (1-4) or (6-9) respectively in the interbend region. The percentage of PR responses increased with the initial local curvature of the resting R bend and the distance of the stimulated site from the flagellar base, and decreased with the age of the preparation.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D006224:Cricetinae; D007478:Iontophoresis; D008297:Male; D008647:Mesocricetus; D008839:Microelectrodes; D009068:Movement / Q000187:drug effects; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D013081:Sperm Motility / Q000187:drug effects; D013082:Sperm Tail / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects*
D011092:Polyethylene Glycols; D000255:Adenosine Triphosphate; D017830:Octoxynol