Ligand binding to protein measured by gel filtration on a dispersion column.
A double conical gel-permeation column is described, which permits an efficient dispersion of solutes in passage. It makes possible a facile determination of ligand binding to protein on the basis of the differential gel permeation of these molecules. The system has been applied, using Sephadex G-25 beads as column packing, to measure [3H]tryptophan binding to bovine serum albumin.
D002850:Chromatography, Gel; D008024:Ligands; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000032:analysis; D014364:Tryptophan / Q000032:analysis
D008024:Ligands; D012710:Serum Albumin, Bovine; D014364:Tryptophan