title: Synthesis and assembly of flagellar components by Caulobacter crescentus motility mutants.
abstract: Cultures of wild-type Caulobacter crescentus and strains with fla mutations representing 24 genes were pulse-labeled with 14C-amino acids and analyzed by immunoprecipitation to study the synthesis of flagellar components. Most fla mutants synthesize flagellin proteins at a reduced rate, suggesting the existence of some mechanism to prevent the accumulation of unpolymerized flagellin subunits. Two strains contain deletions that appear to remove a region necessary for this regulation. The hook protein does not seem to be subject to this type of regulation and, in addition, appears to be synthesized as a faster-sedimenting precursor. Mutations in a number of genes result in the appearance of degradation products of either the flagellin or the hook proteins. Mutations in flaA, -X, -Y, or -Z result in the production of filaments (stubs) that contain altered ratios of the flagellin proteins. In some flaA mutants, other flagellin-related proteins were assembled into the stub structures in addition to the flagellins normally present. Taken together, these analyses have begun to provide insight into the roles of individual fla genes in flagellum biogenesis in C. crescentus.
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D005407:Flagella / Q000378:metabolism* / Q000648:ultrastructure; D005408:Flagellin / Q000096:biosynthesis* / Q000235:genetics; D005798:Genes, Bacterial; D009154:Mutation
chemical_list: D001426:Bacterial Proteins; C035832:hook protein, bacterial flagellum; D005408:Flagellin