Calcium-independent release of [3H]dopamine by veratridine in pargyline- and reserpine-treated corpus striatum.
Veratridine produced a dose-dependent release of cytoplasmic [3H]dopamine in rat corpus striatal slices. Cytoplasmic [3H]dopamine was obtained by pretreating rats with reserpine to inhibit vesicular storage and incubating with pargyline and beta-thujaplicin to inhibit catabolism prior to incubation with [3H]dopamine. Release of [3H]dopamine by veratridine is absolutely calcium-independent and can be abolished by 10(-4) M tetrodotoxin and inhibited by 10(-5) M nomifensine. These results suggest that veratridine can release cytoplasmic [3H]dopamine by a carrier-mediated process.
D000818:Animals; D002118:Calcium / Q000502:physiology*; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D008297:Male; D009627:Nomifensine / Q000494:pharmacology; D010293:Pargyline / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012110:Reserpine / Q000494:pharmacology*; D014701:Veratridine / Q000494:pharmacology*; D014702:Veratrine / Q000031:analogs & derivatives*
D009627:Nomifensine; D014701:Veratridine; D012110:Reserpine; D010293:Pargyline; D014702:Veratrine; D002118:Calcium; D004298:Dopamine