Chromosome damage and SCE induced by the cytostatic factor homo-aza-steroidal ester of P-bis (2-chloro-ethyl) amino phenyl acetic acid in CHO cells in culture.
The cytogenetic effects of homo-aza-steroidal ester (ASE) on Chinese hamster ovary (CHO) cells in vitro are reported. It was found that treatment of cells with a dose as low as 0.025 micrograms/ml of ASE causes a significant number of abnormal metaphases containing mostly chromatid-type aberrations. Similarly, significant frequencies of SCE were induced with only 0.075 micrograms/ml of ASE.
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D001378:Azasteroids; D002460:Cell Line; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D006224:Cricetinae; D003412:Cricetulus; D003434:Crossing Over, Genetic / Q000187:drug effects*; D005260:Female; D009152:Mutagenicity Tests; D009153:Mutagens; D009588:Nitrogen Mustard Compounds / Q000633:toxicity*; D010053:Ovary; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D000970:Antineoplastic Agents; D001378:Azasteroids; D009153:Mutagens; D009588:Nitrogen Mustard Compounds; C033499:3-(((4-(bis(2-chloroethyl)amino)phenyl)acetyl)oxy)-17a-aza-D-homoandrost-5-en-17-one