Effect of heparin on the porcine lymphocyte chromatin--II. Comparative study of sedimentation of chromatin DNA and isolated DNA.
1. Sedimentation of chromatin DNA and isolated deproteinized DNA was compared in neutral and alkaline sucrose density gradients after incubation of chromatin or DNA with various concentrations of heparin. 2. Irrespective of the molecular weight of DNA, an increase in the sedimentation constant of DNA was found with increasing concentration of the polyanion employed.
D000818:Animals; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002621:Chemistry; D002843:Chromatin / Q000302:isolation & purification*; D004247:DNA / Q000302:isolation & purification*; D006493:Heparin / Q000494:pharmacology*; D008214:Lymphocytes / Q000378:metabolism*; D008970:Molecular Weight; D013552:Swine
D002843:Chromatin; D006493:Heparin; D004247:DNA