title: Reorganization in granuloprival cerebellar cultures after transplantation of granule cells and glia. II. Ultrastructural studies.
abstract: Cytosine arabinoside-induced granuloprival cerebellar cultures lack both granule cells and differentiated glia and demonstrate marked synaptic reorganization. After kainic acid-exposed cerebellar explants, which contain granule cells and mature glia, were transplanted to the granuloprival cultures, the following ultrastructural features were noted: (1) parallel fibers formed normal synapses with Purkinje cell dendritic spines as well as with basket/stellate cell somata; (2) sprouted Purkinje cell recurrent axon collateral terminals were markedly reduced in number; (3) Purkinje cells matured and lost perisomatic spines; (4) astroglia formed sheaths around Purkinje cell somata and dendrites; and (5) axonal myelination occurred. The transplanted cultures demonstrated ultrastructural restitution toward normal after addition of missing elements.
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002525:Cerebellar Cortex / Q000166:cytology / Q000648:ultrastructure; D002531:Cerebellum; D051379:Mice; D008854:Microscopy, Electron; D009186:Myelin Sheath / Q000648:ultrastructure; D009457:Neuroglia; D009473:Neuronal Plasticity; D009924:Organ Culture Techniques; D011689:Purkinje Cells / Q000648:ultrastructure