Purification and characterization of exo-beta-1,3-glucanase from a hatching supernatant of Strongylocentrotus intermedius.
Exo-beta-1,3-glucanase from the sea urchin embryos was purified 114-fold from the initial hatching supernatant by the following procedures: (a) gel filtration on Sephadex G-100; (b) hydrophobic chromatography on 4-phenylbutylamine-Sepharose (PBA-Sepharose); (c) two ion-exchange chromatographic steps on DEAE-cellulose; (d) gel filtration on Ultrogel AcA 34; (e) gel filtration on Sephadex G-100. The purified enzyme contained 2.2% carbohydrate and gave a single protein band corresponding to a molecular weight of 136 000 following electrophoresis on sodium dodecyl sulfate (SDS) - urea - polyacrylamide gel. Gel filtration on Ultrogel AcA 34 with a nondenaturing solvent gave a molecular weight of 130 000 +/- 6000. The enzyme displayed an optimum pH at 5.0-5.5 and hydrolysed laminarin and PS(curdlan)-beads at the nonreducing ends, releasing glucose. Although activity of the purified enzyme was not affected by SDS, urea, some divalent ions, and 2-mercaptoethanol, both dithiothreitol and Hg2+ were markedly inhibitory.
D000818:Animals; D001755:Blastocyst / Q000201:enzymology; D004229:Dithiothreitol / Q000494:pharmacology; D043326:Glucan 1,3-beta-Glucosidase; D005947:Glucose / Q000378:metabolism; D005959:Glucosidases / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008628:Mercury / Q000494:pharmacology; D008970:Molecular Weight; D012617:Sea Urchins / Q000196:embryology / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature
D005959:Glucosidases; D043326:Glucan 1,3-beta-Glucosidase; D008628:Mercury; D005947:Glucose; D004229:Dithiothreitol