Novel effective immunoadsorbents based on agarose-polyaldehyde microsphere beads: synthesis and affinity chromatography.
Agarose-polyaldehyde microsphere beads were produced by encapsulating polyacrolein microspheres or polyglutaraldehyde microspheres with agarose. Magnetic beads were formed by carrying out the encapsulation procedure in the presence of ferrofluidic particles. Proteins were bound covalently, at physiological pH, to the beads through their aldehyde groups to produce the Schiff base products. The conjugates, beads-proteins, were used successfully in affinity chromatography for specific purification of antibodies. Leaching of the proteins bound to the beads under physiological conditions and eluting conditions was not detected. The agarose-polyaldehyde microsphere beads are suggested as alternatives to the supports currently used in affinity chromatography.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000936:Antigen-Antibody Complex / Q000138:chemical synthesis*; D002846:Chromatography, Affinity / Q000379:methods*; D007164:Immunosorbents / Q000138:chemical synthesis*; D008863:Microspheres; D011134:Polysaccharides; D012685:Sepharose / Q000031:analogs & derivatives
D000906:Antibodies; D000936:Antigen-Antibody Complex; D007164:Immunosorbents; D011134:Polysaccharides; D012685:Sepharose