Intraarterial and intraportal in vivo catheterization of the regenerating rat liver. Effects upon body and liver weights and DNA synthesis.
A method for concomitant partial hepatectomy and catheterization of the arterial and portal systems of the liver in the rat is described. Catheters were inserted into the gastroduodenal artery and the ileocolic vein. Continuous saline perfusion was performed during 36 hours. In catheterized rats recovery of liver and body weight lagged behind that of non-catheterized rats. The more extensive surgery and the presence of catheters also caused decreased incorporation of 3H-thymidine into liver DNA 24 hours postoperatively. The variation in thymidine incorporation between animals was large. It was shown that by pre-labelling liver DNA with 14C-thymidine the rats can serve as their own controls during acute experiments involving 3H-thymidine, thus reducing the inconsistency of individual variation.
D000818:Animals; D001835:Body Weight; D002404:Catheterization / Q000009:adverse effects / Q000379:methods*; D004247:DNA / Q000096:biosynthesis; D006498:Hepatectomy / Q000009:adverse effects / Q000379:methods*; D008099:Liver / Q000033:anatomy & histology / Q000378:metabolism / Q000502:physiology; D008102:Liver Circulation; D008115:Liver Regeneration; D008297:Male; D009929:Organ Size; D051381:Rats; D011919:Rats, Inbred Strains
D004247:DNA