title: The ultrastructural basis of the permeability of arterial endothelium to horseradish peroxidase. Freeze-fracture and tracer studies of rat thoracic aorta and basilar artery.
abstract: The thoracic aorta and basilar artery, in which the incidence of atherosclerosis is known to be different, were examined to elucidate the correlation between the structure of the intercellular cleft junction between adjacent endothelial cells and its permeability to HRP. Tannic acid or HRP in the vessel lumen passed through the intercellular clefts of the thoracic aorta into the subendothelial space, whereas in the basilar artery they were unable to penetrate beyond the tight junction of the intercellular clefts. Freeze-fracture replicas revealed that the tight junctions of the thoracic aorta consisted of one to two junctional strands in most areas of the cleaved planes, with discontinuities in some places, whereas those of the basilar artery consisted of a continuous belt-like meshwork of six anastomosing junctional strands on average. These observations confirm that the structure of endothelial junctions in arteries has a close correlation with the permeability of the intercellular clefts to HRP.
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D001158:Arteries / Q000378:metabolism / Q000648:ultrastructure*; D001488:Basilar Artery / Q000378:metabolism; D004727:Endothelium / Q000378:metabolism / Q000648:ultrastructure; D005614:Freeze Fracturing; D006735:Horseradish Peroxidase / Q000378:metabolism*; D047348:Hydrolyzable Tannins / Q000494:pharmacology; D008297:Male; D010539:Permeability; D010544:Peroxidases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D047348:Hydrolyzable Tannins; D006735:Horseradish Peroxidase; D010544:Peroxidases