Metabolism of N8-monoacetylspermidine in rat hepatoma cells. Investigation of its effect on the activity of L-ornithine decarboxylase.
Recent evidence has indicated a role for the acetyl derivatives of polyamines, particularly N8-monoacetylspermidine, as activators of L-ornithine decarboxylase in rat hepatoma tissue culture (HTC) cells. This is in contrast with the well-described negative regulatory control of ornithine decarboxylase exerted by their non-acetylated counterparts. Because of the possibility of a rapid extracellular and intracellular catabolism of the acetyl derivatives of polyamines, the metabolism of N8-monoacetylspermidine and its effect on HTC cell ornithine decarboxylase have been investigated, under conditions which eliminate its extracellular catabolism. Differing from previous reports, we demonstrate that N8-monoacetylspermidine does not elevate ornithine decarboxylase activity when added at low concentrations to the culture medium of HTC cells. Higher concentrations decrease ornithine decarboxylase activity in a dose-dependent manner. This effect cannot be unambiguously attributed to the effect of the acetyl derivative itself, because of the presence in situ of a very active N8-monoacetylspermidine deacetylase, which generates spermidine intracellularly.
D000581:Amidohydrolases / Q000378:metabolism; D000818:Animals; D002262:Carboxy-Lyases / Q000378:metabolism*; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D051381:Rats; D013095:Spermidine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology
C030223:N(8)-acetylspermidine; C017988:N(1)-acetylspermidine; D000581:Amidohydrolases; C028411:acetylpolyamine deacetylase; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; D013095:Spermidine