Granulocyte-macrophage progenitor cell preservation at 4 degrees C.
Eighteen bone marrows collected from patients without haematological diseases and from normal subjects were tested for the effects of 4 d storage at 4 degrees C on CFU-C growth. Results indicate that unfractionated bone marrow cells may be stored at 4 degrees C for 4 d with 97% +/- 8 SEM recovery of the CFU-C evaluated by the agar culture assay. On the other hand, the same preservation procedure on peripheral blood CFU-C of 13 normal subjects yielded only 5% +/- 2 SEM recovery of in vitro growth capacity. The present results have practical implications. They might be exploited to preserve bone marrow CFU-C for transplantation therapy or laboratory investigation. In contrast this single preservation procedure seems not appropriate for preserving blood CFU-C.
D001793:Blood Preservation / Q000379:methods; D001854:Bone Marrow Cells; D003114:Colony-Forming Units Assay; D006098:Granulocytes; D006412:Hematopoietic Stem Cells; D006801:Humans; D008264:Macrophages; D012034:Refrigeration; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*