Identification of cell surface polypeptides encoded by human chromosomes 2 and 5 in human-mouse somatic cell hybrids.
Iodinated cell surface polypeptides of human-mouse somatic cell hybrids were analyzed by one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis and high resolution two-dimensional gel electrophoresis in an effort to identify human gene products. Expression of two cell surface polypeptides was positively correlated with retention of specific human chromosomes: a polypeptide of molecular weight 250,000 and isoelectric point 8.3 with the human 5, and a polypeptide of molecular weight 250,000 and isoelectric point 7.0 with the human 2.
D000818:Animals; D002460:Cell Line; D002900:Chromosomes, Human, 1-3; D002905:Chromosomes, Human, 4-5; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D006822:Hybrid Cells / Q000502:physiology; D007041:Hypoxanthine Phosphoribosyltransferase / Q000172:deficiency; D007621:Karyotyping; D008565:Membrane Proteins / Q000235:genetics* / Q000302:isolation & purification; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000235:genetics* / Q000302:isolation & purification
D008565:Membrane Proteins; D010455:Peptides; D007041:Hypoxanthine Phosphoribosyltransferase