Evaluation of a colorimetric method for determination of glycosylated hemoglobin.
We evaluated a colorimetric assay for glycosylated hemoglobin to determine the effects of several variables --oxalic acid concentration, extraneous glucose, hemoglobin concentration, hydrolysis interval, and 5-hydroxymethylfurfural destruction--and the precision. The interference seen when the blood glucose concentration exceeds 2.0 g/L (11 mmol/L) can be eliminated by washing the erythrocytes with 9 g/L saline. The accuracy of this assay is not influenced by hemoglobin concentrations from 80 to 150 g/L. The background nonspecific color, although substantial, is similar from sample to sample. After a 5-h hydrolysis at 100 degrees C, about 85% of the hexose is released, and the analytical recovery of 5-hydroxymethylfurfural is constant over a wide range of glycosylated hemoglobin concentrations. The 5th to 95th percentile reference interval for a population of 65 nondiabetic individuals was 4.6 to 6.1 mol per 100 mol of total hemoglobin. The range of values for a population of 85 diabetic patients was 6.9 to 20.4 mol per 100 mol.
D001786:Blood Glucose; D003124:Colorimetry / Q000379:methods; D003920:Diabetes Mellitus / Q000097:blood*; D004912:Erythrocytes / Q000032:analysis*; D005069:Evaluation Studies as Topic; D005662:Furaldehyde / Q000031:analogs & derivatives; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D006868:Hydrolysis; D010070:Oxalates; D013997:Time Factors
D001786:Blood Glucose; D005662:Furaldehyde; D006442:Glycated Hemoglobin; D010070:Oxalates; C008046:5-hydroxymethylfurfural