Stimulation of the zona-free hamster ova penetration efficiency by human spermatozoa after 17 beta-estradiol treatment.
17 beta-Estradiol (E2) stimulated the zona-free hamster ova penetration by human spermatozoa from both fertile and subfertile men after 5 hours of incubation in vitro. For spermatozoa samples from fertile men, the mean penetration rates increased significantly (P less than 0.05), from 35.6% +/- 3.5% (control) to 60.1% +/- 5.8% (E2 = 50 ng/ ml) and 50.5% +/- 2.6% (E2 = 100 ng/ml), respectively. E2 also increased the mean penetration rates of spermatozoa samples from subfertile men significantly (P less than 0.05), from 10.2% +/- 1.8% (control) to 30.5% +/- 7.2% (E2 = 50 ng/ml) and 33.1% +/- 6.6% (E2 = 100 ng/ml), respectively. These findings indicate that E2 can affect the fertilizing ability of human spermatozoa in vitro.
D000818:Animals; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005306:Fertilization / Q000187:drug effects*; D005307:Fertilization in Vitro / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D007247:Infertility, Female / Q000209:etiology; D007315:Insemination, Artificial; D008297:Male; D010063:Ovum / Q000187:drug effects; D013084:Sperm-Ovum Interactions / Q000187:drug effects*; D013094:Spermatozoa / Q000502:physiology*
D004958:Estradiol