Selective uptake of [3H]glutamine and [3H]glutamate into neurons and satellite cells of dorsal root ganglia in vitro.
The uptake of [3H]glutamate and [3H]glutamine into rat dorsal root ganglia has been examined by autoradiography and thin-layer chromatography. [3H]glutamate was selectively accumulated by satellite glial cells and after 10 min, 53% of this had been converted to [3H]glutamine. [3H]glutamine, on the other hand, entered neuronal perikarya and 40% was converted to [3H]glutamate. It is suggested that these selective uptake processes provide supporting evidence for the existence of a neuronal-glial glutamine cycle in dorsal root ganglia. Small dark (B) cells accumulated 6 times as much [3H]glutamine as did large light (A) cells. The reasons for this marked difference in the metabolism of the two main types of dorsal root ganglion neurone are discussed.
D000818:Animals; D001345:Autoradiography; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D066298:In Vitro Techniques; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D005971:Glutamates; D005973:Glutamine; D014316:Tritium