The precursor of rat liver mitochondrial glutamate dehydrogenase has enzymatic activity.
The cytosolic precursor for the mitochondrial glutamate dehydrogenase of rat liver was synthesized in a cell-free reticulocyte lysate using messenger RNA from rat liver. To check whether this precursor had enzymatic activity, a highly sensitive fluorimetric method, which can measure picogram quantities of enzyme, was used together with competitive dissociation of the precursor from an immunoprecipitate with inactive glutamate dehydrogenase. Glutamate dehydrogenase activity, corresponding to that estimated from incorporation of [35S]-methionine, was detected in the precursor. The significance of this finding is discussed.
D000818:Animals; D002474:Cell-Free System; D055598:Chemical Phenomena; D002621:Chemistry; D003600:Cytosol / Q000201:enzymology; D004792:Enzyme Precursors / Q000378:metabolism*; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D007120:Immunochemistry; D008930:Mitochondria, Liver / Q000201:enzymology*; D012333:RNA, Messenger / Q000502:physiology; D051381:Rats; D013050:Spectrometry, Fluorescence
D004792:Enzyme Precursors; D012333:RNA, Messenger; D005969:Glutamate Dehydrogenase