Cytopathogenicity of Naegleria fowleri for rat neuroblastoma cell cultures: scanning electron microscopy study.
Neuroblastoma cells were inoculated with Naegleria fowleri Lee and examined for cytopathology at various periods post-inoculation by scanning electron microscopy. By 18 h post-inoculation, approximately 50% of neuroblastoma cells were nonviable, as evidenced by trypan blue exclusion and light microscopic examination. This cytopathology resulted from piecemeal consumption of target cells mediated by a sucker apparatus extending from the surface of N. fowleri.
D000656:Amoeba / Q000502:physiology* / Q000648:ultrastructure; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D002470:Cell Survival; D003593:Cytoplasm / Q000648:ultrastructure; D006790:Host-Parasite Interactions; D008855:Microscopy, Electron, Scanning; D009447:Neuroblastoma; D009474:Neurons / Q000166:cytology / Q000469:parasitology*; D051381:Rats