Natural killer cell activity in adult gnotobiotic dogs.
An in vitro assay for natural killer cell activity by canine peripheral blood leukocytes was developed, using chromium-51-labeled heterologous (Vero) and homologous (Madin-Darby canine kidney, canine melanoma, and canine thyroid adenocarcinoma) target cells. Transformed tumor cell lines proved highly susceptible to natural killer-mediated lysis. Leukocytes from adult gnotobiotic dogs possessed levels of activity comparable to that observed in conventional animals.
D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology; D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004285:Dogs / Q000276:immunology*; D005260:Female; D005856:Germ-Free Life; D006801:Humans; D007668:Kidney; D007694:Killer Cells, Natural / Q000276:immunology*; D008297:Male; D008545:Melanoma / Q000276:immunology / Q000473:pathology; D013552:Swine / Q000276:immunology; D013964:Thyroid Neoplasms / Q000276:immunology / Q000473:pathology