High-performance liquid chromatographic analysis of the new hypoxic cell radiosensitiser, Ro 03-8799, in biological samples.
A high-performance liquid chromatographic method of analysis with UV detection has been developed to measure levels of a new radiosensitiser, Ro 03-8799 and its N-oxide metabolite, in biological fluids and tissues. The accuracy and precision of the method have been determined in both plasma and urine, where the limits of quantitation are 100 and 500 ng/ml, respectively. Typical results are presented from a human volunteer study where samples were analysed by this method. Important aspects of the method, involving both sample handling techniques and chromatographic conditions are discussed.
D000818:Animals; D001646:Bile / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004912:Erythrocytes / Q000032:analysis; D006801:Humans; D009369:Neoplasms / Q000032:analysis; D009593:Nitroimidazoles / Q000032:analysis* / Q000378:metabolism; D010100:Oxygen; D011838:Radiation-Sensitizing Agents / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D009593:Nitroimidazoles; D011838:Radiation-Sensitizing Agents; C033815:pimonidazole; C038185:Ro 31-0313; D010100:Oxygen