title: Purification and characterization of the RNA polymerases of the sea urchin, Lytechinus variegatus.
abstract: We have identified four forms of sea urchin RNA polymerase (Ia, Ib, II and III). Three of the forms co-elute on DEAE cellulose chromatography but separate on DEAE Sephadex chromatography. The separation of these three enzyme forms by DEAE Sephadex chromatography can be eliminated with non-ionic detergent. We also demonstrate that either form I or form III RNA polymerase loses its resistance to alpha-amanitin after DEAE chromatography. A procedure for the purification of combined form I and III RNA polymerase and the purification of RNA polymerase II is also presented.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology*; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification*; D012318:RNA Polymerase I / Q000302:isolation & purification; D012319:RNA Polymerase II / Q000302:isolation & purification; D012320:RNA Polymerase III / Q000302:isolation & purification; D012617:Sea Urchins / Q000196:embryology / Q000201:enzymology*
chemical_list: D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I; D012320:RNA Polymerase III