title: Characterization of Toxoplasma gondii antigens that react with human immunoglobulin M and immunoglobulin G antibodies.
abstract: Studies were performed to define the nature of the Toxoplasma gondii antigens that are recognized by human immunoglobulin M (IgM) and IgG antibodies. Both IgM and IgG antibodies were found to be directed mainly against T. gondii membrane antigens in sera obtained from patients with acute toxoplasmosis. Treatment of the membrane preparation with DNase, RNase, or lipase had no apparent effect on the reactivity of the membrane antigens with IgM and IgG antibodies. Lipids isolated from tachyzoites were not recognized by either IgM or IgG antibodies. Exposure of T. gondii membranes to heat, proteolysis, or oxidation with sodium periodate decreased the reactivity of the membrane preparations with both IgM and IgG antibodies. A preparation of T. gondii proteins and polysaccharides were recognized by both immunoglobulin classes. T. gondii polysaccharides reacted with human IgG antibodies produced during both the acute and chronic phases of the infection. We concluded that, after infection with T. gondii, IgM and IgG antibodies are elicited in response to both protein and carbohydrate constituents of the invading parasite.
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology*; D003851:Deoxyribonucleases / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D008049:Lipase / Q000494:pharmacology; D008055:Lipids / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology; D011134:Polysaccharides / Q000276:immunology; D012260:Ribonucleases / Q000494:pharmacology; D014122:Toxoplasma / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008055:Lipids; D008565:Membrane Proteins; D011134:Polysaccharides; D003851:Deoxyribonucleases; D012260:Ribonucleases; D008049:Lipase