Evaluation of a chromatographic method for globin chain biosynthesis in thalassemia.
We report our experience with a column chromatographic procedure for separating 3H-leucine-labelled alpha and beta globin. In non-thalassemic subjects the alpha: beta biosynthesis ratio was 1.04 +/- 0.10 S.D. An abnormal ratio was useful in defining thalassemia variants. The technique, although labour intensive, is not difficult and is recommended for any hospital laboratory acting as a reference centre for the diagnosis of hemoglobinopathies.
D002852:Chromatography, Ion Exchange / Q000379:methods; D005069:Evaluation Studies as Topic; D005914:Globins / Q000096:biosynthesis* / Q000302:isolation & purification; D006454:Hemoglobins / Q000096:biosynthesis; D008297:Male; D013789:Thalassemia / Q000097:blood* / Q000175:diagnosis
D006454:Hemoglobins; D005914:Globins