title: Identification of three indolic compounds in a pigmented-melanoma cell-culture supernatant by gas chromatography-mass spectrometry.
abstract: In the supernatant of melanoma cell culture SK mel 25 three indolic compounds - 5-hydroxy-6-methoxyindole, 5-hydroxy-6-methoxyindolyl-2-carboxylic, and 6-hydroxy-5-methoxyindolyl-2-carboxylic acids - have been identified. The supernatants were extracted with ethyl acetate, derivatized, and analyzed by gas chromatography-mass spectrometry. The significance of this finding is discussed.
mesh_terms: D002478:Cells, Cultured; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007211:Indoles / Q000032:analysis*; D008545:Melanoma / Q000032:analysis*; D012878:Skin Neoplasms / Q000032:analysis*
chemical_list: D007211:Indoles; C038282:5-hydroxy-6-methoxyindole; C017407:5-hydroxy-6-methoxy-2-indolylcarboxylic acid; C017408:6-hydroxy-5-methoxy-2-indolylcarboxylic acid