Hemoglobin Okayama [beta 2 (NA 2) His replaced by Gln]: a new 'silent' hemoglobin variant with substituted amino acid residue at the 2,3-diphosphoglycerate binding site.
A new 'silent' abnormal hemoglobin, Hb Okayama [beta 2 (NA 2) His replaced by Gln], happened to be discovered in a diabetic Japanese female living in Okayama Prefecture, Japan, in the course of glyco-Hb measurement of the blood samples of diabetic patients. This variant did not differ from Hb A by conventional electrophoretic tests. Only the isoelectric focusing on PAG plate for the determination of glyco-Hb and the cation exchanger chromatography were successful in the separation of this abnormal variant from Hb A and glyco-Hb. Functional study of the whole blood demonstrated a slight increase of oxygen affinity.
D019794:2,3-Diphosphoglycerate; D000595:Amino Acid Sequence; D003920:Diabetes Mellitus / Q000097:blood; D004163:Diphosphoglyceric Acids / Q000097:blood*; D005260:Female; D005973:Glutamine; D006455:Hemoglobins, Abnormal / Q000302:isolation & purification* / Q000378:metabolism; D006639:Histidine; D006801:Humans; D007525:Isoelectric Focusing; D007564:Japan; D008875:Middle Aged; D010100:Oxygen / Q000097:blood
D004163:Diphosphoglyceric Acids; D006455:Hemoglobins, Abnormal; D005973:Glutamine; D019794:2,3-Diphosphoglycerate; D006639:Histidine; D010100:Oxygen