Agglutination assay of bladder cells by Concanavalin A proved the high susceptibility of analbuminemic rats to bladder carcinogens.
The susceptibility of analbuminemic rats, a mutant strain of Sprague-Dawley rats, to bladder carcinogens was examined by testing the agglutinability of isolated bladder epithelial cells by Concanavalin A (Con A). One-week treatment with 0.001% N-butyl-N-(4-hydroxybutyl)nitrosamine (BHBN) in the drinking water or 0.01% N-(4-(5-nitro-2-furyl)-2-thiazolyl)-formamide (FANFT), 0.01% and 0.1% 4-aminobiphenyl, 0.1% 4-nitrobiphenyl, 0.1% benzidine, 0.1% 2-napthylamine or 5% sodium saccharin in the diet clearly induced high agglutinability of bladder cells of male analbuminemic rats but not Sprague-Dawley rats. These results suggest that male analbuminemic rats are very susceptible to bladder carcinogens in general and therefore will be very useful in screening tests for bladder carcinogens.
D015081:2-Naphthylamine / Q000633:toxicity; D000372:Agglutination Tests; D000611:Aminobiphenyl Compounds / Q000633:toxicity; D000818:Animals; D002085:Butylhydroxybutylnitrosamine / Q000633:toxicity; D002273:Carcinogens; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D004032:Diet; D004198:Disease Susceptibility; D004353:Drug Evaluation, Preclinical; D005200:FANFT / Q000633:toxicity; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012439:Saccharin / Q000633:toxicity; D012709:Serum Albumin / Q000172:deficiency*; D001743:Urinary Bladder / Q000187:drug effects*; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced*
D000611:Aminobiphenyl Compounds; D002273:Carcinogens; D012709:Serum Albumin; D003208:Concanavalin A; C006757:4-biphenylamine; D002085:Butylhydroxybutylnitrosamine; D005200:FANFT; D015081:2-Naphthylamine; D012439:Saccharin