Estimation of protein secondary structure from the laser Raman amide I spectrum.
A new method for estimating protein secondary structure from the laser Raman spectrum has been developed whereby the amide I Raman band of a protein is analyzed directly as a linear combination of amide I bands of proteins whose secondary structures are known. For 14 proteins, analyzed by removing each one from the reference set and analyzing its structure in terms of the remaining proteins, the average correlation coefficients between the Raman and X-ray diffraction estimates of helix, beta-strand, turn, and undefined were 0.98, 0.98, 0.82 and 0.35, respectively. Significant correlations were also observed for distinctions between alpha-helix (0.98) and disordered helix (0.82), and between parallel (0.82) and antiparallel (0.97) beta-sheets. The average standard deviation of these Raman estimates from the X-ray values is less than 4%. In addition, a singular value analysis of 20 Raman amide I spectra indicates that there may be as many as nine significant independent pieces of information present in the amide I region.
D000577:Amides; D000704:Analysis of Variance; D007834:Lasers; D009113:Muramidase; D011487:Protein Conformation; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013223:Statistics as Topic; D013820:Thermolysin; D014961:X-Ray Diffraction
D000577:Amides; D009113:Muramidase; D013820:Thermolysin