Ultrastructural and histochemical study of the gallbladder epithelia of rainbow trout and tench.
The simple, high columnar gallbladder epithelia of the rainbow trout (Salmo gairdneri, Rich.) and the tench (Tinca tinca, L.) are composed of light and dark cells. Within the dark cells two types must be distinguished: less hydrated, but normal epithelial cells and degenerating cells. The latter seem to be eliminated from the epithelium through phagocytosis by monocytes and macrophages, which possibly correspond with the 'basal regenerative cells' of earlier investigations. Since they are mainly found near the basement membrane, an hypothesis is put forth that they do not enter the gallbladder lumen, but migrate back into the subepithelial connective tissue after phagocytosis. Light and less hydrated dark cells are specialized for absorption of water and dissolved bile salts and for secretion of mucus, which mainly consists of acid glycoproteins. Interspecific differences are seen with regard to both functions. So, in the tench only single microvilli are developed apically, whereas a true brush border is seen in the rainbow trout. Thus, a higher absorptive rate can be assumed in the rainbow trout. Or, with respect to mucus secretion, in the rainbow trout the granular endoplasmic reticulum predominates, whereas in the tench the smooth endoplasmic reticulum, often associated with glycogen and even forming 'glycogen bodies', is better developed. In the rainbow trout fibrillar granules of highly condensed acid glycoproteins are seen to be secreted. In the tench only vesicles of flocculent content occur. Perhaps, these morphological and subsequent metabolic differences may be related to the various feeding habits of the predatory rainbow trout and the omnivorous tench. Seasonal variations as expected especially in the tench after winter rest could not be established.
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004848:Epithelium / Q000032:analysis / Q000648:ultrastructure; D005399:Fishes / Q000033:anatomy & histology*; D005704:Gallbladder / Q000032:analysis / Q000648:ultrastructure*; D006023:Glycoproteins / Q000032:analysis; D006651:Histocytochemistry; D008854:Microscopy, Electron; D008871:Microvilli / Q000648:ultrastructure; D008938:Mitosis; D009940:Organoids / Q000648:ultrastructure; D012487:Salmonidae / Q000033:anatomy & histology*; D014337:Trout / Q000033:anatomy & histology*
D006023:Glycoproteins