Dose and time dependent effects of morphine on the incorporation of [3H]valine into soluble brain and liver proteins.
Morphine (10(-6)-10(-5) M) causes an increase in incorporation of [3H]valine into soluble proteins during 4 hr in rat brain cortical slices, liver slices and cultivated astroglial cells. The effects are dose-dependent. They are neither cell specific nor strictly related to classical opiate receptors. Pulse-labeling with [3H]valine for 60 min after incubation in 10(-6)-10(-5) M morphine, resolves time-dependent changes in incorporation, with both increases and decreases in protein metabolism.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009020:Morphine / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009928:Organ Specificity; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium; D014633:Valine / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D014316:Tritium; D009020:Morphine; D014633:Valine