title: Toxic, DNA-damaging and mutagenic activity of epichlorohydrin on human cells cultured in vitro.
abstract: Epichlorohydrin (ECHH) highly inhibited the tritiated thymidine uptake by human lymphocytes cultured in vitro, although the corresponding cell viability was unaffected. Furthermore, it elicited unscheduled DNA synthesis, acting as a DNA-damaging agent after its metabolic activation. ECHH also showed a clear toxic and mutagenic activity toward a human epithelial-like cell line, causing a decrease in cell viability and an increase in mutants resistant to 0.05 Lf/ml of diphtheria toxin.
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002728:Chlorohydrins / Q000633:toxicity*; D004247:DNA / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects; D004167:Diphtheria Toxin / Q000037:antagonists & inhibitors; D004351:Drug Resistance; D004811:Epichlorohydrin / Q000633:toxicity*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D013936:Thymidine / Q000378:metabolism
chemical_list: D002728:Chlorohydrins; D004167:Diphtheria Toxin; D009153:Mutagens; D004811:Epichlorohydrin; D004247:DNA; D013936:Thymidine