Fractionation and kinetic properties of rat liver and kidney methionine adenosyltransferase isozymes.
Three isozymes of methionine adenosyltransferase (EC 2.5.1.6; MAT-I, -II, and -III) exist in normal rat liver and are conveniently purified (MAT-III to homogeneity) by a three-step column chromatography procedure. MAT-I shows Michaelis-Menten kinetics with a Km (L-methionine) of 41 microM and a molecular weight of 208 000 and is slightly inhibited by S-adenosyl-L-methionine (Adomet). MAT-II, which is also the only isozyme found in normal rat kidney, shows negative cooperativity with a Hill coefficient of 0.7. It has a L-methionine concentration required for half-maximal velocity [S0.5(Met)] of 8 microM and a molecular weight of 120 000 and is strongly inhibited by Adomet. MAT-I and -II comprise 15% and 5%, respectively, of total MAT activity in rat liver. The predominant isozyme in rat liver, MAT-III, demonstrates positive cooperativity with a Hill coefficient of 1.8. It has a molecular weight of 97 000 and apparently consists of two subunits of identical molecular weight (47 000). This liver-specific isozyme is strongly activated by both dimethyl sulfoxide and Adomet and has a S0.5(Met) of 215 microM.
D000818:Animals; D002852:Chromatography, Ion Exchange; D005260:Female; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008716:Methionine Adenosyltransferase / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D014166:Transferases / Q000302:isolation & purification*
D007527:Isoenzymes; D014166:Transferases; D008716:Methionine Adenosyltransferase