Simultaneous detection of some fusariotoxins by gas-liquid chromatography.
A method was developed for the simultaneous determination of 6 Fusarium mycotoxins (deoxynivalenol, diacetoxyscirpenol, HT-2 toxin, T-2 toxin, fusarenon-x, and zearalenone). Cereal samples were first extracted with ethyl acetate, then with a mixture of methanol-water. The crude extracts were combined and purified by silica gel chromatography. The purified extract was reacted with BSTFA (N,O-bis(trimethylsilyl)-trifluoroacetamide) to form the derivative, and chromatographed on an SE-52 wall-coated open tubular column. Kováts' retention index was determined for the 6 mycotoxins investigated. Recoveries and standard deviations were determined for pure toxin mixed in cereal. Recovery was 70-80%; relative standard deviation was 10-18%. The method developed was applied to different cereal samples.
D002849:Chromatography, Gas; D002523:Edible Grain / Q000032:analysis*; D009183:Mycotoxins / Q000032:analysis*; D013605:T-2 Toxin / Q000032:analysis; D014908:Triticum / Q000032:analysis
D009183:Mycotoxins; D013605:T-2 Toxin