Stimulation of increased capillary endothelial cell motility by chondrosarcoma cell-derived factors.
The ability of chondrosarcoma cell-derived preparations to stimulate an increase in the motility of bovine capillary endothelial (BCE) cells was quantitated using a phagokinetic track assay which measures the area of tracks produced by BCE cells after ingestion of gold particles. Chondrosarcoma preparations stimulated a 2-fold increase in the mean track area produced by BCE cells in an 18-hr incubation period. The motility-stimulating activity of chondrosarcoma was purified about 120-fold. The most highly purified fractions had molecular weights between 16,000 and 20,000 and stimulated a 2-fold increase in BCE cell motility at concentrations of 10 to 20 ng/ml.
D000818:Animals; D002196:Capillaries / Q000187:drug effects* / Q000502:physiology; D002417:Cattle; D002457:Cell Extracts / Q000032:analysis / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D002467:Cell Nucleus / Q000378:metabolism; D002813:Chondrosarcoma / Q000378:metabolism*; D002843:Chromatin / Q000302:isolation & purification; D002850:Chromatography, Gel; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D005260:Female; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical; D014020:Tissue Extracts / Q000494:pharmacology*
D002457:Cell Extracts; D002843:Chromatin; D014020:Tissue Extracts