Muscarinic cholinergic receptors on circulating human lymphocytes: evidence for a particulate binding site.
Lymphocytes from thirteen normal volunteers were investigated for their muscarinic cholinergic receptors content. A particular binding site was characterized with 3H-quinuclidinyl benzilate; saturation binding occurred at 80 X 10(-9)M and scatchard analysis provided a dissociation constant of 40 X 10(-9)M. These results showed that there were about 4000 muscarinic receptors on human lymphocyte. Although, it is possible to demonstrate the presence of binding site on a particulate preparation, the significance of such low-affinity receptor is yet unclear.
D000328:Adult; D001285:Atropine / Q000494:pharmacology; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism*; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000378:metabolism*
D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate; D001285:Atropine