Failure of various agents to decrease oleic acid pulmonary albumin leak.
Computerized pulmonary gamma scintigraphy has been shown to be a sensitive technique for the measurement of albumin flux in oleic acid pulmonary microvascular injury. In this technique technetium-99m-tagged human serum albumin is administered intravenously and lung:heart radioactivity ratios are constructed. This ratio remains constant unless there is a net flux of albumin from the vascular space into the lung, when a rising ratio is seen, called the "slope of injury" or SI. Gamma scintigraphy provides a method to rapidly screen the ability of various possible therapeutic agents to reduce the flux of albumin in experimental ARDS. In this study, 0.05 ml/kg oleic acid produced a significant increase in the SI. None of the agents tested (30 mg/kg methylprednisolone, 12.5 mg/kg ibuprofen, 4 mg/kg MK-447, a superoxide radical scavenger, or 140 mg/kg calcium gluconate) were able to alter the scintigraphically measured increased albumin flux produced by 0.05 ml/kg oleic acid.
D000418:Albumins / Q000378:metabolism*; D000818:Animals; D002084:Butylated Hydroxytoluene / Q000031:analogs & derivatives / Q000494:pharmacology; D002125:Calcium Gluconate / Q000494:pharmacology; D004285:Dogs; D007052:Ibuprofen / Q000494:pharmacology; D008168:Lung / Q000000981:diagnostic imaging / Q000187:drug effects*; D008775:Methylprednisolone / Q000494:pharmacology; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism*; D011877:Radionuclide Imaging
D000418:Albumins; D009829:Oleic Acids; D002084:Butylated Hydroxytoluene; D019301:Oleic Acid; C013753:2-aminomethyl-4-t-butyl-6-iodophenol; D002125:Calcium Gluconate; D007052:Ibuprofen; D008775:Methylprednisolone