Simultaneous determination of protein (nitrogen), phosphorus, and calcium in animal feedstuffs by multichannel flow-injection analysis.
A 3-channel flow-injection procedure was developed, which enables the simultaneous determination of protein, phosphorus, and calcium in a wide range of animal feeds from a single digestion. Samples are digested with a block digestor, diluted, and analyzed at a rate of 82 samples/h. Protein (nitrogen) as ammonia is determined colorimetrically by the indophenol method. Phosphorus and calcium are determined by measuring the absorbances of the molybdenum blue and calcium-cresolphthalein complexes at 660 and 580 nm, respectively. Protein is determined in the range from 0 to 75%, phosphorus in the range from 0 to 6%, and calcium in the range from 0 to 6%. The results obtained do not differ significantly from those obtained by proven manual methods, and considerable time, space, and reagents are saved.
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001322:Autoanalysis / Q000295:instrumentation; D002118:Calcium / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D004044:Dietary Proteins / Q000032:analysis*; D009584:Nitrogen / Q000032:analysis; D010758:Phosphorus / Q000032:analysis*; D010940:Plant Proteins / Q000032:analysis
D004044:Dietary Proteins; D010940:Plant Proteins; D010758:Phosphorus; D009584:Nitrogen; D002118:Calcium