Interaction of unconjugated bilirubin with brush border vesicles from proximal tubules of rat kidney.
An interaction between non conjugated bilirubin and brush border vesicles was found which does not change the absorbance spectrum of bilirubin, nor protects it against the action of peroxidase. It seems to reach saturation at about 20 min., and appears to be mainly a superficial binding, probably non specific and relatively weak. The bound amount of bilirubin was high and can not be neglected in any interpretation of the reabsorption of the solute by proximal tubules.
D000818:Animals; D001663:Bilirubin / Q000378:metabolism*; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry; D013997:Time Factors
D003470:Culture Media; D001663:Bilirubin