Induction of a stress protein in developing cell cultures of the rat cerebellum.
We examined the ability of developing cerebellar cell cultures to synthesize a 71,000 MW stress protein (SP71) in response to heat shock and Cd2+ treatment. The induction of SP71 synthesis appeared to be dependent on both the age of the culture and the stressor used. Heat shock induced SP71 synthesis in freshly prepared cells and in cell cultures at each age examined, whereas Cd2+ was effective only in cultures at 7 days of age and older. These findings are discussed with reference to the development of various cell types in these cultures.
D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000502:physiology*; D006360:Heat-Shock Proteins; D006358:Hot Temperature; D008970:Molecular Weight; D014176:Protein Biosynthesis; D051381:Rats
D006360:Heat-Shock Proteins