Immobilization of rat brain synaptic vesicles on positively-charged glass microspheres.
Synaptic vesicles from rat brain were immobilized on glass microspheres covalently coated with poly-L-lysine. Using a potassium tartrate perfusion medium, the vesicular accumulation and methamphetamine-induced release of (L)-3H-norephinephrine could be conveniently monitored in a flow experiment.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D005260:Female; D005898:Glass; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D009638:Norepinephrine / Q000378:metabolism; D010477:Perfusion / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D013572:Synaptic Vesicles / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
D008694:Methamphetamine; D009638:Norepinephrine