High-performance liquid chromatographic determination of alpha-keto acids in plasma with fluorometric detection.
This paper describes a sensitive high-performance liquid chromatographic method for the quantitative determination of alpha-keto acids in plasma using a fluorescence detector. This method is about ten times more sensitive than that reported in a previous paper. Only 50 microliters of plasma are needed for the determination of alpha-keto acids. However, p-hydroxyphenylpyruvic acid could not be analysed because the quinoxalinol derived from it does not exhibit fluorescence.
D002851:Chromatography, High Pressure Liquid; D005470:Fluorometry; D006801:Humans; D007231:Infant, Newborn; D007651:Keto Acids / Q000097:blood*; D008375:Maple Syrup Urine Disease / Q000097:blood; D010655:Phenylenediamines
D007651:Keto Acids; D010655:Phenylenediamines