title: Mechanism of the glycine cleavage reaction. Steady state kinetic studies of the P-protein-catalyzed reaction.
abstract: Chicken liver P-protein of the multienzyme glycine cleavage system catalyzes the first partial reaction of glycine cleavage. In the partial reaction, glycine and H-protein serve as substrates and the products are CO2 (not bicarbonate) and the decarboxylated portion of glycine attached to H-protein. The reaction exhibited Michaelis-Menten kinetics with respect to both substrates. The optimum pH for the reaction is 7.1, with 6.5 for the reverse reaction. Km values for glycine and H-protein are independent of the concentration of the the co-substrate, and calculated values are 5.8 mM for glycine and 3.4 microM for H-protein. Initial velocity experiments gave intersecting double reciprocal plots that conform to a sequential mechanism. Product inhibition studies revealed that both products inhibited competitively with respect to the varied substrate. Glycine methyl ester was found to be a competitive inhibitor of glycine and noncompetitive inhibitor of H-protein. H-protein whose lipoic acid prosthetic group and cysteinyl residues were modified with N-ethylmaleimide was a noncompetitive inhibitor of glycine and a competitive inhibitor of H-protein. These results are most consistent with a sequential random Bi Bi mechanism in which no abortive dead end complex is formed. This was supported by an isotope exchange experiment.
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002645:Chickens; D050961:Glycine Decarboxylase Complex H-Protein; D050841:Glycine Dehydrogenase (Decarboxylating); D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology*
chemical_list: D002352:Carrier Proteins; D050961:Glycine Decarboxylase Complex H-Protein; D000594:Amino Acid Oxidoreductases; D050841:Glycine Dehydrogenase (Decarboxylating)