76-kDa poly(A)-protein is involved in the formation of 48 S initiation complexes.
In erythropoietic mouse cells induced by Friend leukemia virus, approximately 50% of non-polyribosomal globin mRNA is found in 48 S initiation complexes ready to be translated. EDTA releases 15 S globin mRNPs, homologous to polyribosomal globin mRNPs. The 76-kDa poly(A)-protein is one of its main protein components. The other 50% of non-polyribosomal message can be separated as 20 S 'free' mRNPs. Its protein composition is different, especially the 76-kDa protein is lacking. The role of this protein is discussed.
D000818:Animals; D005622:Friend murine leukemia virus; D005914:Globins / Q000096:biosynthesis; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D010442:Peptide Chain Initiation, Translational; D011061:Poly A / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000096:biosynthesis
D012333:RNA, Messenger; D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein; D011061:Poly A; D005914:Globins