title: Pathogenesis of Trypanosoma brucei infection in deer mice (Peromyscus maniculatus): hematologic, erythrocyte biochemical, and iron metabolic aspects.
abstract: Trypanosoma brucei EATRO 110 infection in deer mice (Peromyscus maniculatus) produced anemia in 15 of 42 mice between postinoculation days 14 and 70. The infected anemic (IA) mice had significantly higher reticulocyte counts (P less than 0.025), spleen (P less than 0.001) and liver (P less than 0.005) weights, and higher parasitemia than did infected nonanemic (INA) mice. gamma-Globulin concentrations of infected mice were markedly increased, and values for INA mice were 10% higher than values for IA mice. Erythrocyte hexokinase, glucose-6-phosphate dehydrogenase, glutathione peroxidase, glutathione reductase, and pyruvate kinase activities were increased in infected mice, whereas phosphofructokinase was only slightly decreased in infected mice. Seemingly, development of anemia was not related to defects in erythrocyte metabolism. Serum iron values of infected mice were similar to those of controls. Storage iron (hemosiderin and ferritin) concentrations were increased in the spleen and to a lesser extent in the liver. The activity of superoxide dismutase, an enzyme that favors conversion of easily mobilized soluble ferritin to poorly mobilized insoluble hemosiderin, was decreased per unit weight of the enlarged spleen, although total activity was increased. The superoxide dismutase activity per unit weight of liver was not altered in infected mice although total liver activities were increased. These findings, as well as the marked reticulocytosis, indicate that lack of iron supply does not have a part in precipitating the anemia of T brucei infection. Leukocytosis was present in infected animals and was associated with lymphocytosis, eosinopenia, basophilia, and monocytosis; these changes were more marked in IA than in INA mice.
mesh_terms: D000740:Anemia / Q000097:blood / Q000201:enzymology / Q000378:metabolism / Q000662:veterinary*; D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D004195:Disease Models, Animal; D004906:Erythrocyte Count / Q000662:veterinary; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D007501:Iron / Q000378:metabolism*; D007958:Leukocyte Count / Q000662:veterinary; D008297:Male; D010542:Peromyscus / Q000097:blood*; D012156:Reticulocytes; D012376:Rodent Diseases / Q000097:blood* / Q000201:enzymology / Q000209:etiology; D013482:Superoxide Dismutase / Q000378:metabolism; D014346:Trypanosoma brucei brucei; D014353:Trypanosomiasis, African / Q000097:blood / Q000201:enzymology / Q000209:etiology / Q000662:veterinary*
chemical_list: D001798:Blood Proteins; D007501:Iron; D013482:Superoxide Dismutase