The kinetic measurement of phagocyte function in whole blood.
A technique has been developed for the kinetic measurement on the same blood sample of a variety of functions of phagocytic cells. Adherence to glass, the clearance of a mixture of microorganisms and their subsequent solubilisation, and the secretion of granule contents from the cells were determined. The numbers of residual viable intracellular staphylococci were measured at the completion of the study. These methods were used to investigate phagocyte function in 33 normal subjects and 6 patients with chronic granulomatous disease.
D000293:Adolescent; D000328:Adult; D001769:Blood; D001770:Blood Bactericidal Activity; D001792:Blood Platelets / Q000502:physiology; D002448:Cell Adhesion; D005260:Female; D006105:Granulomatous Disease, Chronic / Q000097:blood / Q000382:microbiology; D006801:Humans; D007700:Kinetics; D007781:Lactoferrin / Q000097:blood; D007958:Leukocyte Count; D008297:Male; D009000:Monocytes / Q000502:physiology; D009504:Neutrophils / Q000502:physiology; D010586:Phagocytes / Q000276:immunology* / Q000502:physiology; D013211:Staphylococcus aureus / Q000378:metabolism
D007781:Lactoferrin