Pressure microinjection of nerve growth factor and anti-nerve growth factor into the nucleus and cytoplasm: lack of effects on neurite outgrowth from pheochromocytoma cells.
Exogenous proteins have been introduced into naive and nerve growth factor (NGF)-primed pheochromocytoma cells by pressure microinjection. Microinjection of cells did affect NGF-induced neurite outgrowth by these cells over periods of up to 3 days. Injected NGF failed to elicit neurite outgrowth or regeneration in the absence of extracellular NGF and failed to enhance it in its presence. Injected anti-NGF IgG failed to inhibit neurite outgrowth or regeneration induced by extracellular NGF. Similar results were obtained for both nuclear and cytoplasmic injections.
D000818:Animals; D002467:Cell Nucleus; D002999:Clone Cells; D003593:Cytoplasm; D008845:Microinjections; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000503:physiopathology; D009414:Nerve Growth Factors / Q000008:administration & dosage / Q000378:metabolism*; D009416:Nerve Regeneration; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D010673:Pheochromocytoma / Q000378:metabolism / Q000503:physiopathology; D012377:Rodentia
D009414:Nerve Growth Factors