Cytokinetic and cytogenetic effect of agricultural chemicals on human lymphoid cells in vitro. II. Organochlorine pesticides.
Human lymphoid cells of LAZ-007 cell line, incubated with 10(-4) to 10(-6) molar of eight different organochlorine pesticides had dose related cytotoxicity, mitotic depression and cell cycle traverse inhibition. In cultures incubated with 10(-4) M concentrations, M1 metaphases were as high as 13% (Dicofol) as compared to less than 1% in the controls. The frequency of M3 metaphases in cultures incubated with 10(-6) M concentrations ranged from 11% (Chlordane) to 15% (Endosulfan) compared to 17% in control cultures. Statistically significant increase in SCE frequency was seen in cells exposed to Chlordane, Dicofol, Endosulfan and Toxaphene. On metabolic activation with rat liver microsomal S-9 enzymes, Chlordane, Dicofol and Tetradifon induced SCE frequency was higher than that of nonactivated cultures.
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006582:Hexachlorophene / Q000633:toxicity; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D051381:Rats; D012854:Sister Chromatid Exchange / Q000187:drug effects; D014112:Toxaphene / Q000633:toxicity
D007306:Insecticides; D014112:Toxaphene; D006582:Hexachlorophene