Transport and processing of staphylococcal enterotoxin B.
A larger, membrane-bound form of staphylococcal enterotoxin B was shown by in vivo pulse-chase analysis to be the kinetic precursor to extracellular enterotoxin B. Processing of the enterotoxin B precursor molecules can apparently occur either cotranslationally or posttranslationally. Subcellular fractionation of cells revealed that all of the precursor toxin was associated with the membrane fraction. Once processed and released from the membrane, it was transiently associated with the cell wall before being released into the extracellular environment. The cell-wall-associated enterotoxin B was completely resistant to protease treatment and to extraction by high- or low-salt solutions at 0 to 2 degrees C, although it could be easily released from the cell by removal of the cell wall with lysostaphin. These data imply that newly formed enterotoxin B may be temporarily sequestered in specialized regions that require cell wall integrity before being released into the extracellular environment.
D019297:2,4-Dinitrophenol; D001692:Biological Transport; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D004140:Dinitrophenols / Q000494:pharmacology; D004768:Enterotoxins / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D013211:Staphylococcus aureus / Q000378:metabolism*; D014107:Tosyllysine Chloromethyl Ketone / Q000494:pharmacology
D004140:Dinitrophenols; D004768:Enterotoxins; D014107:Tosyllysine Chloromethyl Ketone; C031818:enterotoxin B, staphylococcal; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D019297:2,4-Dinitrophenol