Gas-liquid chromatographic evaluation of fenquizone in biological samples for pharmacokinetic investigations.
Extractive alkylation was carried out on fenquizone, a sulphonamide diuretic, in order to devise a suitable method for its determination in pharmacokinetic and bioavailability studies. After extraction as a tetramethyl derivative, fenquizone was evaluated by gas-liquid chromatography with a 63Ni electron-capture detector, which enables a limit of detection of 2 ng/ml of plasma or urine to be achieved. Linearity was verified in a range of 50- 10,000 pg for each injection with a fenquizone/internal standard ratio ranging from 4:1 to 1:4. Determination is very rapid, as one analysis only takes 5 min. The preliminary results of the pharmacokinetic study performed in a volunteer human subject after a single oral administration of the drug are presented in this paper in terms of the plasma levels and the cumulative urinary excretion.
D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D007700:Kinetics; D013058:Mass Spectrometry; D008745:Methylation; D011799:Quinazolines / Q000032:analysis* / Q000378:metabolism; D013997:Time Factors
D011799:Quinazolines; C007712:fenquizone