Enhanced Detection of immunoglobulin binding by a modified ELISA.
A modification of the ELISA assay is described which significantly enhances the chromogenic signal, thereby increasing the sensitivity of the assay. This enhanced ELISA employs a protein A-HRP conjugate to detect antibody bound to antigen coated polystyrene plates, followed by incubation with an anti-protein A-HRP conjugate as the amplification step. This enhanced ELISA is rapid, inexpensive, uses commercially available reagents and maintains the low background and high specificity of standard ELISA.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay; D011817:Rabbits; D011863:Radioimmunoassay; D013205:Staphylococcal Protein A / Q000276:immunology
D000911:Antibodies, Monoclonal; D013205:Staphylococcal Protein A