[Addition of albumin to solutions of cholecystokinin (CCK)--effect on pancreatic secretion, plasma CCK level and release of pancreatic polypeptide (PP)].
Dissolving basic peptides such as insulin, secretin, and somatostatin in albumin-containing solutions increases the biologic potency. As CCK-33 also constitutes a basic peptide, it should have similar qualities. In four conscious dogs with chronic gastric and pancreatic Thomas fistulas, we studied the effect of CCK-33 solutions with and without addition of albumin on pancreatic secretin, plasma PP concentration, CCK-like immunoreactivity in plasma and infusion lines. The response of pancreatic protein output (mg/15 min) to increasing doses of 99% pure CCK-33 (0.5; 1; 2; 4 IDU/kg/h) was significantly higher (p less than 0.05) when CCK was dissolved in 0.154 M NaCl with albumin than in NaCl alone. These results were confirmed by measuring plasma CCK-immunoreactivity by CCK-RIA and CCK-immunoreactivity in samples from tips of infusion lines by gastrin-RIA. CCK evoked PP release at increasing doses of CCK-33, which was significantly (p less than 0.05) higher when CCK was dissolved in an albumin-containing solution. There was a significant (p less than 0.02) correlation between plasma PP concentration and pancreatic protein output. Our study shows addition of albumin to solutions of CCK-33 to cause an increase of CCK-33 activity, probably by preventing adsorption of the peptide to glass and plastic surfaces. This is valid for its effect on pancreatic protein secretion as well as PP release.
D000418:Albumins / Q000494:pharmacology*; D000818:Animals; D002766:Cholecystokinin / Q000097:blood* / Q000494:pharmacology*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism; D010191:Pancreatic Polypeptide / Q000097:blood*; D010446:Peptide Fragments / Q000494:pharmacology*; D011863:Radioimmunoassay
D000418:Albumins; D010446:Peptide Fragments; D010191:Pancreatic Polypeptide; D002766:Cholecystokinin
Zusatz von Albumin zu Lösungen von Cholezystokinin (CCK)--Wirkung und Pankreassekretion, Plasma-CCK-Spiegel und Freisetzung von pankreatischem Polypeptid (PP).