title: Polymorphism in clathrin light chains from different tissues.
abstract: Two types of light chains were observed in clathrins from different bovine tissues. Clathrin was purified from seven different bovine tissues using immunoaffinity columns prepared from a monoclonal antibody specific for the heavier light chain (LCa) of bovine brain clathrin. The LCa equivalent in clathrin from non-brain tissue was identified by monoclonal antibody binding to electrophoretic blots of these clathrins. The LCa and LCb light chains of bovine brain clathrin are, respectively, 4 X 10(3) daltons and 3 X 10(3) daltons heavier than their corresponding light chains in other tissues. The light chains from tissues other than brain are similar in molecular weight.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001923:Brain Chemistry; D002417:Cattle; D002846:Chromatography, Affinity; D002966:Clathrin; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000276:immunology; D008970:Molecular Weight
chemical_list: D000911:Antibodies, Monoclonal; D002966:Clathrin; D008565:Membrane Proteins