Demonstration of S2-receptor binding sites on cat blood platelets using [3H]ketanserin.
[3H]Ketanserin showed substantial binding to cat platelet membranes; 40% of this was specific binding, characterized by a plateau in serotonin antagonist inhibition curves, and another 40% was non-specific binding to structural recognition sites displaceable by unlabelled ketanserin. Specific [3H]ketanserin binding to platelets shows the features of serotonin S2-receptor binding sites previously identified in rat pre-frontal cortex and in striatum. In platelets, KD = 1.02 nM and Bmax = 86 fmol/10(9) platelets; the Ki values of 21 compounds were similar in the three different tissues.
D000818:Animals; D001665:Binding Sites; D001792:Blood Platelets / Q000032:analysis* / Q000378:metabolism; D002415:Cats; D003342:Corpus Striatum / Q000032:analysis; D005260:Female; D066298:In Vitro Techniques; D007650:Ketanserin; D008297:Male; D010880:Piperidines / Q000378:metabolism*; D051381:Rats; D011985:Receptors, Serotonin / Q000032:analysis*; D012702:Serotonin Antagonists / Q000378:metabolism*; D014316:Tritium
D010880:Piperidines; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; D014316:Tritium; D007650:Ketanserin