Enzyme-linked lectin assay (ELLA): use of alkaline phosphatase-conjugated Griffonia simplicifolia B4 isolectin for the detection of alpha-D-galactopyranosyl end groups.
Alkaline phosphatase has been coupled to Griffonia simplicifolia I B4 isolectin using a one-step glutaraldehyde conjugation procedure. This enzyme-lectin conjugate (AP-GS I-B4) has been used to specifically detect plastic-bound natural and synthetic glycoproteins bearing alpha-D-galactopyranosyl end groups. The extent of reactivity of the AP-GS I-B4 with the glycoproteins appears to be proportional to the number of terminal galactosyl residues present. Furthermore, this assay, termed ELLA (enzyme-linked lectin assay), is specifically inhibitable by low-molecular-weight sugars containing terminal alpha-D-galactosyl groups. The ELLA reactions may be assayed rapidly and objectively by the use of commercially available ELISA-plate using standard filters.
D000469:Alkaline Phosphatase; D000818:Animals; D002417:Cattle; D004285:Dogs; D005697:Galactosides / Q000032:analysis*; D037161:Galectins; D006023:Glycoproteins / Q000302:isolation & purification; D006027:Glycosides / Q000032:analysis*; D037102:Lectins
D005697:Galactosides; D037161:Galectins; D006023:Glycoproteins; D006027:Glycosides; D037102:Lectins; D000469:Alkaline Phosphatase