Myositis autoantibody inhibits histidyl-tRNA synthetase: a model for autoimmunity.
In autoimmune disorders such as rheumatoid arthritis and systemic lupus erythematosus (SLE), autoantibodies are generated against a variety of macromolecules. Myositis is a human autoimmune disease characterized by weakness and wasting of muscle. In American studies, antibodies directed against soluble cellular constituents were detected by immunodiffusion in about 60% of cases; the commonest of these, found in 25% of patients, was antibody to the Jo-1 antigen. An antibody system referred to as PL-1 was recognized at a similar frequency in a series of patients studied at Hammersmith Hospital, London. We show here that this system is identical with the Jo-1 system and demonstrate that the antigen is a polypeptide of molecular weight (Mr) 50,000. The protein is immunoprecipitated with tRNA His and appears to be histidyl-tRNA synthetase. The identity of the Jo-1 antigen, the first of the RNA-associated antigens familiar in autoimmune disease to be characterized as a specific enzyme, suggests a model for virus involvement in autoantibody generation.
D000604:Amino Acyl-tRNA Synthetases / Q000276:immunology*; D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D001327:Autoimmune Diseases / Q000276:immunology*; D003882:Dermatomyositis / Q000276:immunology; D006367:HeLa Cells / Q000276:immunology; D006643:Histidine-tRNA Ligase / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances; D008970:Molecular Weight; D009220:Myositis / Q000276:immunology*
D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D046911:Macromolecular Substances; D000604:Amino Acyl-tRNA Synthetases; D006643:Histidine-tRNA Ligase