Effect of perfluorodecalin on the microsomal mono-oxygenase system in perfused rat livers.
1. An emulsion of perfluorodecalin was used as a substitute for erythrocytes in a synthetic medium in the perfusion of isolated rat livers. 2. The efficiency of this oxygen carrier in maintaining liver viability and function was compared with an erythrocyte-containing medium. 3. Activities of the microsomal mono-oxygenase system, secretion of bile and concn. of transaminases, potassium, glucose, urea, pO2 and pCO2 in the medium, and ATP and glycogen in the tissue, did not differ in the two experimental conditions.
D000255:Adenosine Triphosphate / Q000032:analysis; D000410:Alanine Transaminase / Q000032:analysis; D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis; D001802:Blood Substitutes / Q000494:pharmacology*; D005466:Fluorocarbons / Q000494:pharmacology*; D008112:Liver Glycogen / Q000032:analysis; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D010100:Oxygen / Q000097:blood; D010105:Oxygenases / Q000032:analysis*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D001802:Blood Substitutes; D005466:Fluorocarbons; D008112:Liver Glycogen; C008805:perfluorodecalin; D000255:Adenosine Triphosphate; D010105:Oxygenases; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D010100:Oxygen