Mutation induction by cross-links in DNA of Deinococcus radiodurans.
Reversion of adenine auxotrophic strains of D. radiodurans by cross-links in DNA was studied. Mutation was induced in a wild-type but not in a mitomycin C sensitive mutant after irradiation with near UV (approximately 365nm) in the presence of 4,5',8-trimethylpsoralen (TMP). To get information on implication of cross-links in the observed mutation, cells containing monoadducts in DNA were exposed to near UV in the absence of TMP. The mutation frequency of the wild-type increased with increasing the cross-links in DNA. These results suggest that structural defects after removal of cross-links in DNA induce mutation in D. radiodurans.
D055598:Chemical Phenomena; D002621:Chemistry; D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism* / Q000528:radiation effects; D009154:Mutation
D004269:DNA, Bacterial