Inactivation of Ricin using 4-azidophenyl-beta-D-galactopyranoside and 4-diazophenyl-beta-D-galactopyranoside.
4-Azidophenyl-beta-D-galactopyranoside and 4-diazophenyl-beta-D-galactopyranoside were used to inactivate ricin. Galactose, but not glucose, protected against inactivation as measured by the retention of the ability of ricin to bind to Bio-Gel A, a galactose-containing gel. Nearly complete inhibition of binding to Bio-Gel A, to monosaccharides, or to cell surface receptors could be achieved by reaction of ricin with either label, but neither label impaired the ability of the A chain to inhibit translation in vitro. The diazonium salt-modified ricin still inhibited cellular protein synthesis, but ricin modified by the photoactivated label was 280 times less efficient than ricin in inhibition of cellular protein synthesis.
D000818:Animals; D001386:Azides / Q000494:pharmacology*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003979:Diazonium Compounds / Q000494:pharmacology*; D007700:Kinetics; D007942:Leukemia, Experimental / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000187:drug effects; D051381:Rats; D012270:Ribosomes / Q000378:metabolism; D012276:Ricin / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D013329:Structure-Activity Relationship
D001386:Azides; D003979:Diazonium Compounds; C038246:4-azidophenylgalactoside; C038247:4-diazophenylgalactoside; D012276:Ricin