Determination of Leu-enkephalin degradation by a soluble enzyme preparation from calf-brain striatum using reversed-phase high-performance liquid chromatography.
A rapid procedure for the determination of Leu-enkephalin (Tyr-Gly-Gly-Phe-Leu) and its main metabolic degradation products (Tyr, Tyr-Gly-Gly and Tyr-Gly) by reversed-phase high-performance liquid chromatography was developed. The method has good precision, the coefficient of variation determined in the range 6-20 pmole being 1.5-3% (n = 8), and a very low detection limit of ca. 10 fmole for each metabolite. An unexpectedly high percentage of Tyr-Gly production is observed after enzymatic degradation of Leu-enkephalin by a solubilized enzyme preparation of calf-brain striatum.
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000201:enzymology*; D004743:Enkephalin, Leucine / Q000378:metabolism*; D005998:Glycine / Q000032:analysis; D006868:Hydrolysis; D012995:Solubility; D014443:Tyrosine / Q000032:analysis
D014443:Tyrosine; D004743:Enkephalin, Leucine; D005998:Glycine