Use of lyophilized skin for testing the bactericidal activity of teat disinfectants.
The bactericidal activities of various concentrations of 3 disinfectants commonly used for teat disinfection were compared in vitro using small discs of pig skin previously contaminated with Staphylococcus aureus. Solutions containing 40 g/l Na hypochlorite, 25 or 50 g/l chlorhexidine digluconate or iodophor containing 5 g/l iodine were found to have equivalent or superior bactericidal activity to a solution containing 10 g/l Na hypochlorite. This in vitro technique offers a convenient alternative to in vivo tests for teat disinfectants and with minor modifications could be applicable to the testing of skin antiseptics in general.
D000818:Animals; D002417:Cattle; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000494:pharmacology; D004202:Disinfectants / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods / Q000662:veterinary*; D005260:Female; D005612:Freeze Drying; D007466:Iodophors / Q000494:pharmacology; D008321:Mammary Glands, Animal; D012867:Skin / Q000382:microbiology*; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification; D013552:Swine
D004202:Disinfectants; D007466:Iodophors; D012973:Sodium Hypochlorite; C010882:chlorhexidine gluconate; D002710:Chlorhexidine