[Activity of oxidative enzymes of the tricarboxylic acid cycle in the liver of rats during hypokinesia].
The activity of oxidative enzymes of the Krebs cycle was examined in white rats during hypokinesia. On hypokinesia day 7 the cytosol activity of NAD-dependent isocitrate dehydrogenase (ICDH) increased and that of malic-enzyme decreased. On hypokinesia days 30 and 45 the activity of succinate dehydrogenase (SDH) and alpha-ketoglutarate dehydrogenase (alpha-KGDH) decreased, that of cytoplasmatic malate dehydrogenases (MDH) slightly increased, and that of NADP ICDH declined. On hypokinesia day 60 the total activity of mitochondrial dehydrogenases reduced due to a low protein content of the mitochondrial fraction, whereas the specific activity either remained unchanged (ICDH, NAD MDH, alpha-KGDH) or increased (SDH, NADP MDH). On recovery day 25 only the activity of mitochondrial NAD-dependent malate and isocitrate dehydrogenases returned to normal.
D000818:Animals; D002952:Citric Acid Cycle; D003600:Cytosol / Q000201:enzymology*; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism; D008099:Liver / Q000648:ultrastructure*; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D051381:Rats; D012149:Restraint, Physical; D013385:Succinate Dehydrogenase / Q000378:metabolism
D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D007655:Ketoglutarate Dehydrogenase Complex; D013385:Succinate Dehydrogenase
Aktivnost' okislitel'nykh fermentov tsikla trikarbonovykh kislot v pecheni krys pri gipokinezii.