Cardioplegic arrest in isolated blood-perfused working rat hearts.
An isolated working rat heart model designed for function studies after elective cardiac arrest is described. Before and after a cold ischemic period (induced with St. Thomas' cardioplegic solution) of 30 min, hemodynamic and metabolic variables were assessed in a group of 13 hearts perfused with a modified Krebs-Henseleit solution and in a group of 13 hearts perfused with reconstituted blood. Functional recovery was about 90% in the nonblood-perfusate group and 98% in the blood-perfused group. Functional deterioration in time due to edema formation and limited oxygenation in electrolyte-perfused hearts was found to be of the same magnitude as the function loss ascribed to deleterious effects of cardioplegic arrest. The blood-perfused hearts showed hemodynamic stability during a 75 min continuous working period, and complete recovery of function after 30 min arrest.
D000818:Animals; D001769:Blood; D003326:Coronary Circulation; D004573:Electrolytes; D005112:Extracorporeal Circulation; D006321:Heart / Q000502:physiology*; D006324:Heart Arrest, Induced / Q000379:methods*; D006439:Hemodynamics; D007036:Hypothermia, Induced; D008297:Male; D008954:Models, Biological; D009206:Myocardium / Q000378:metabolism; D010101:Oxygen Consumption; D051381:Rats; D011919:Rats, Inbred Strains
D004573:Electrolytes