title: Cell osmotic water permeability of isolated rabbit proximal convoluted tubules.
abstract: Cell osmotic water permeability, Pcos, of the peritubular aspect of the proximal convoluted tubule (PCT) was measured from the time course of cell volume changes subsequent to the sudden imposition of an osmotic gradient, delta Cio, across the cell membrane of PCT that had been dissected and mounted in a chamber. The possibilities of artifact were minimized. The bath was vigorously stirred, the solutions could be 95% changed within 0.1 s, and small osmotic gradients (10-20 mosM) were used. Thus, the osmotically induced water flow was a linear function of delta Cio and the effect of the 70-microns-thick unstirred layers was negligible. In addition, data were extrapolated to delta Cio = 0. Pcos for PCT was 41.6 (+/- 3.5) X 10(-4) cm3 X s-1 X osM-1 per cm2 of peritubular basal area. The standing gradient osmotic theory for transcellular osmosis is incompatible with this value. Published values for Pcos of PST are 25.1 X 10(-4), and for the transepithelial permeability Peos values are 64 X 10(-4) for PCT and 94 X 10(-4) for PST, in the same units. These results indicate that there is room for paracellular water flow in both nephron segments and that the magnitude of the transcellular and paracellular water flows may vary from one segment of the proximal tubule to another.
mesh_terms: D000818:Animals; D001834:Body Water / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology; D007687:Kidney Tubules, Proximal / Q000502:physiology*; D007700:Kinetics; D008433:Mathematics; D009997:Osmotic Pressure; D010539:Permeability; D011817:Rabbits; D012965:Sodium Chloride / Q000378:metabolism; D012996:Solutions
chemical_list: D012996:Solutions; D012965:Sodium Chloride