[Evaluation of the toxic action of prophylactic and therapeutic preparations on cell cultures. III. The detection of toxic properties in medical biological preparations by the degree of cell damage in the L132 continuous cell line].
The methods of the quality control of medical biological preparations, including tests on animals, do not ensure the complete absence of toxicity in a final product. The use of the method of "subcultures with the introduced preparation" makes it possible to determine the toxicity of both specific and nonspecific components of vaccines and sera from the number of dead and damaged cells. The toxic action of preparations kills and damages the cells at the site of injection, thus inducing the formation of autoantigens whose effect on the body cannot be predicted. Thus thimerosal, commonly used as preservative, has been found not only to render its primary toxic effect, but also capable of changing the properties of cells. This fact suggests that the use of thimerosal for the preservation of medical biological preparations, especially those intended for children, is inadmissible.
D000485:Allergens / Q000633:toxicity; D000818:Animals; D001428:Bacterial Vaccines / Q000633:toxicity; D001688:Biological Products / Q000633:toxicity*; D002460:Cell Line; D002478:Cells, Cultured; D007136:Immunoglobulins / Q000633:toxicity; D007739:L Cells / Q000187:drug effects*; D008722:Methods; D051379:Mice; D011786:Quality Control; D013849:Thimerosal / Q000633:toxicity; D014765:Viral Vaccines / Q000633:toxicity
D000485:Allergens; D001428:Bacterial Vaccines; D001688:Biological Products; D007136:Immunoglobulins; D014765:Viral Vaccines; D013849:Thimerosal
Otsenka toksicheskogo deĭstviia profilakticheskikh i lechebnykh preparatov na kul'turakh kletok. Soobshchenie III. Vyiavlenie toksicheskikh svoĭstv v meditsinskikh biologicheskikh preparatakh po stepeni povrezhrdeniia kletk v perebvivaemoĭ linii L132.