Properties of narrow-range ampholytes isolated from wide-range ampholyte preparations.
A simple procedure for obtaining useful narrow-pH-range ampholytes from inexpensive laboratory-synthesized ampholytes by preparative isoelectric focusing in Pevikon is described. The narrow-range ampholytes prepared in this way are comparable to commercial ampholyte preparations as judged by conductivity, buffer capacity, pH gradient formation, and resolving power. These inexpensive narrow-range ampholytes are particularly well suited to preparative isoelectric focusing applications requiring large quantities of ampholytes.
D000665:Ampholyte Mixtures / Q000302:isolation & purification*; D002021:Buffers / Q000302:isolation & purification*; D004553:Electric Conductivity; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods
D000665:Ampholyte Mixtures; D002021:Buffers