Conformations of fortimicins and three-dimensional structure-activity relationship in the aminoglycoside antibiotics.
The fortimicins are pseudodisaccharide antibiotics consisting of purpurosamine and fortamine moieties which are connected through an alpha-linkage. NMR spectra indicate that the fortamine ring in fortimicin A takes a chair conformation which corresponds to an inverted one in the conformation of fortimicin B free base. Fortimicin A is unstable in solution, and we were unable to obtain any crystals of it. To elucidate the structure of fortimicin A and to clarify the stereochemistry of fortimicins, we have undertaken to calculate their empirical force-fields starting from the X-ray structures of fortamines and fortimicin B free base. Nuclear Overhauser enhancement data were also employed to confirm the calculated relative orientation of the two rings around the glycosidic linkage. The 3-dimensional structure-activity relationship of aminoglycoside antibiotics is discussed on the basis of these results.
D000617:Aminoglycosides / Q000032:analysis; D000900:Anti-Bacterial Agents; D002236:Carbohydrate Conformation; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D013329:Structure-Activity Relationship
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; C023385:fortimicin B; C023384:fortimicin A