title: A reagin-like antibody in horse serum: 1. Occurrence and some biological properties.
abstract: The demonstration of a reagin-like antibody against Culicoides pulicaris extract in the serum of horses and ponies affected with recurrent seasonal dermatitis (sweet itch) is reported. This antibody can confer Prauznitz-Küstner (P-K) sensitivity on homologous skin for up to 5 days and, like human IgE, is thermolabile and susceptible to 2-mercaptoethanol reduction. It is eluted on diethylaminoethyl dextran-52 anion exchange chromatography independently of IgG, IgG(T) and IgM, and its elution characteristics indicate similarity in net molecular charge to human IgE. The P-K response observed in horse skin is biphasic, and is morphologically similar to the late cutaneous anaphylactic response in man. Both phases of the P-K response are dependent upon the reagin-like antibody, although other serum factors appear involved in the delayed phase of the response.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000302:isolation & purification*; D002519:Ceratopogonidae / Q000276:immunology; D055598:Chemical Phenomena; D002621:Chemistry; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D003876:Dermatitis, Atopic / Q000276:immunology / Q000662:veterinary*; D006734:Horse Diseases / Q000276:immunology*; D006736:Horses; D007073:Immunoglobulin E / Q000302:isolation & purification; D007428:Intradermal Tests; D011935:Reagins / Q000032:analysis; D012008:Recurrence; D012621:Seasons; D012867:Skin / Q000276:immunology
chemical_list: D000906:Antibodies; D011935:Reagins; D007073:Immunoglobulin E