Determination of benzalkonium chloride in the presence of interfering alkaloids and polymeric substrates by reverse-phase high-performance liquid chromatography.
A specific assay for the analysis of benzalkonium chloride in the presence of interfering substances was conducted. The approach involved complexing benzalkonium chloride in an ophthalmic system with methyl orange, extraction of the complex into 1,2-dichloroethane, and subsequent analysis by reverse-phase high-performance liquid chromatography. Since the method separates each homologue of benzalkonium chloride, homologues not resident in the ophthalmic system were added as internal standards to improve both recovery and precision in the method.
D000470:Alkaloids / Q000032:analysis*; D001548:Benzalkonium Compounds / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D005079:Excipients; D011108:Polymers / Q000378:metabolism*
D000470:Alkaloids; D001548:Benzalkonium Compounds; D004338:Drug Combinations; D005079:Excipients; D011108:Polymers