A simple micromethod for leukocyte rosetting without cell purification.
Conventional methods for leukocyte rosetting suffer from 2 major drawbacks: the length of time required for leukocyte purification prior to rosetting, and the necessity for large volumes of blood from which to purify the leukocytes. A simple method for leukocyte rosetting is described which uses small quantities (less than 100 microliter) of heparinized whole blood, does not require prior leukocyte purification and can be completed within 1 h of blood drawing. Percentages of rosetting polymorphonuclear leukocytes in cell preparations obtained by standard purification methods showed a close correlation (P less than 0.001) with percentages obtained by the method described. Additionally, the method described can potentially be applied to other non-neutrophil rosetting leukocytes in blood or body fluids.
D002469:Cell Separation; D004903:Erythrocyte Aggregation; D006801:Humans; D007962:Leukocytes / Q000145:classification / Q000166:cytology / Q000378:metabolism*; D008860:Microscopy, Ultraviolet; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism; D012397:Rosette Formation / Q000379:methods*