Identification of a sperm receptor-glycoprotein from the porcine zona pellucida.
Porcine zona pellucida were solubilized by treatment with lithium 3,5-diiodosalicylate. Following further purification by phenol/water extraction, ethanol precipitation and high performance liquid chromatography a total of 11 fractions were obtained. Fractions in the molecular mass range, that were previously found to possess antigenic properties, were tested in a spermatozoa radio-receptor binding assay. One of the fractions with an N-terminal amino-acid residue of alanine was found to have spermatozoa receptor properties. This result might aid future studies on the spermatozoazona pellucida interactions at the molecular level.
D000596:Amino Acids / Q000032:analysis; D000606:Amino Sugars / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D006868:Hydrolysis; D007457:Iodine Radioisotopes; D008297:Male; D010063:Ovum / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism*; D013552:Swine; D014357:Trypsin; D015044:Zona Pellucida / Q000378:metabolism*
D000596:Amino Acids; D000606:Amino Sugars; D006023:Glycoproteins; D007457:Iodine Radioisotopes; D014357:Trypsin