Retinol increases synthesis and secretion of Sertoli cell mannose-containing glycoproteins.
Sertoli cell isolated from prepuberal rats were cultured in a chemically defined medium in the presence or in the absence or retinol. In the two experimental conditions the incorporation of 3H-mannose into cellular glycoproteins was measured. The results obtained demonstrated a 3 fold increase of mannose incorporation into cellular glycoproteins after retinol addition without any qualitative variation of their electrophoretic profile. Sertoli cell secretory activity increased of approximately 50% after retinol administration; the electrophoretic analysis showed that two of the secretory products were selectively enhanced by the vitamin addition.
D000818:Animals; D002478:Cells, Cultured; D005944:Glucosamine / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis* / Q000378:metabolism; D008297:Male; D008358:Mannose / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism*; D014801:Vitamin A / Q000494:pharmacology*
D006023:Glycoproteins; D014801:Vitamin A; D005944:Glucosamine; D008358:Mannose