Fluorographic detection of radioactivity in polyacrylamide gels with 2,5-diphenyloxazole in acetic acid and its comparison with existing procedures.
A fluorographic procedure was optimized which utilized acetic acid as the solvent for 2,5-diphenyloxazole (PPO). This procedure was then compared with existing fluorographic procedures which utilize PPO in dimethyl sulphoxide or sodium salicylate in water, and a commercially available fluorographic solution. En3Hance (New England Nuclear Corp.). A comparison of the four methods revealed that all of the procedures resulted in essentially the same efficiency of radioactivity detection. The acetic acid/PPO procedure was found to have several technical advantages. There is no need to pre-fix proteins in gels, and either agarose or acrylamide gels can be used. The acetic acid/PPO procedure was found to be a simple, sensitive and efficient alternative fluorographic method.
D000085:Acetates; D019342:Acetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D005471:Fluoroscopy / Q000379:methods*; D010080:Oxazoles; D010779:Photofluorography / Q000379:methods*; D011506:Proteins / Q000032:analysis
D000085:Acetates; D010080:Oxazoles; D011506:Proteins; C009012:2,5-diphenyloxazole; D019342:Acetic Acid