Coupling ratio of electrogenic Na+-alanine cotransport in isolated rat hepatocytes.
Na+-alanine cotransport across the cell membrane in isolated rat hepatocytes was studied. Changes in the cell membrane potential associated with the transport of alanine interfere with determination of the Na+-alanine coupling ratio of the cotransport. With valinomycin present to 'clamp' the cell membrane potential, a coupling ratio of 1:1 for the Na+-alanine influx was obtained.
D000409:Alanine / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism*; D014634:Valinomycin / Q000494:pharmacology
D014634:Valinomycin; D012964:Sodium; D000409:Alanine