Liquid-chromatographic determination of nadolol in plasma.
We describe a liquid-chromatographic procedure for determining nadolol in plasma. After an analog of nadolol is added as internal standard, the plasma sample is passed through a disposable BondElut C18 column. After several column washes, nadolol and the internal standard are eluted with methanol, and the eluate is evaporated and reconstituted with the mobile phase (acetonitrile/water, perchloric acid, and tetramethylammonium hydroxide). An aliquot of the extract is chromatographed on a non-silica resin-base reversed-phase column. The peaks are detected by fluorescence (lambda ex = 265 nm and lambda em = 305). Drug and internal standard are well resolved, and only a few extraneous peaks appear. The standard curve ranges from 10 to 400 micrograms/L. We are using this procedure to determine steady-state concentrations of nadolol in patients receiving various dosages of nadolol along with other types of antihypertensive drugs.
D000959:Antihypertensive Agents / Q000097:blood; D002853:Chromatography, Liquid; D006801:Humans; D007674:Kidney Diseases / Q000097:blood* / Q000188:drug therapy; D009248:Nadolol / Q000031:analogs & derivatives; D011412:Propanolamines / Q000097:blood* / Q000302:isolation & purification / Q000627:therapeutic use; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D000959:Antihypertensive Agents; D011412:Propanolamines; D009248:Nadolol; C038273:SQ 23554