Influence of physicochemical parameters on adsorption of Actinomyces viscosus to hydroxyapatite surfaces.
Adsorption of Actinomyces viscosus strains T14V and T14AV to saliva-treated and untreated hydroxyapatite (HA) under various environmental conditions was studied. Strain T14V adsorption to saliva-treated HA was not influenced by pH, whereas strain T14AV adsorbed in higher numbers under acidic conditions. The addition of cations inhibited adsorption of strains T14V and T14AV to saliva-treated HA. Strain T14V possessed a greater affinity for hydrophobic gels than did strain T14AV, but incorporation of non-ionic detergents, which inhibit hydrophobic interactions, did not influence adsorption of strain T14V to saliva-treated HA. Adsorption of strain T14V to saliva-treated HA increased in the presence of an anionic detergent; however, strain T14AV adsorption was not affected. Strain T14V adsorption decreased in the presence of a cationic detergent, whereas strain T14AV adsorption increased. Collectively, these data suggest that electrostatic interactions, but not hydrophobic interactions, are of major importance in the adsorption of strain T14V to saliva-treated HA.
D000190:Actinomyces / Q000502:physiology*; D000327:Adsorption; D000328:Adult; D002118:Calcium / Q000494:pharmacology; D002122:Calcium Chloride / Q000494:pharmacology; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006882:Hydroxyapatites; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D008297:Male; D009994:Osmolar Concentration; D011189:Potassium Chloride / Q000494:pharmacology; D012463:Saliva / Q000502:physiology*
D002593:Cetrimonium Compounds; D003902:Detergents; D006882:Hydroxyapatites; D015636:Magnesium Chloride; D011189:Potassium Chloride; D008274:Magnesium; D002122:Calcium Chloride; D002118:Calcium; D000077286:Cetrimonium