Cytochemical analysis of human peripheral blood lymphocyte subsets defined by monoclonal antibodies.
Human blood lymphocyte subpopulations, revealed by a panel of commercially available monoclonal antibodies by means of a rosetting technique, were submitted to direct cytochemical analysis and were shown to have distinguishing characteristics. T cells reactive with OKT3 antibody (T3+) displayed higher beta-glucuronidase and dot-like alpha-naphthyl acetate acid esterase (ANAE) activity than T3- cells. Helper/inducer cells (T4+) were characterized by a high level of dot-like ANAE activity, whereas cytotoxic/suppressor cells (T8+) displayed selective naphthol-ASD-chloroacetate esterase activity. These results provide evidence for association of some cytoplasmic enzyme activities with the expression of membrane differentiation markers defined by monoclonal antibodies.
D000135:Acid Phosphatase / Q000097:blood; D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D001773:Blood Cells / Q000145:classification*; D003593:Cytoplasm / Q000201:enzymology; D005966:Glucuronidase / Q000097:blood; D006801:Humans; D008214:Lymphocytes / Q000145:classification / Q000201:enzymology* / Q000276:immunology; D009283:Naphthol AS D Esterase / Q000097:blood; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D009283:Naphthol AS D Esterase; D000135:Acid Phosphatase; D005966:Glucuronidase