Response to a single dose of human chorionic gonadotropin in prepubertal boys.
In normal men a single dose of hCG induces an increase in plasma testosterone (T) and 17 alpha-hydroxyprogesterone (17 alpha-OHP) 2-4 h after the injection; after 24-36 h a maximum increase in plasma 17 beta-estradiol (E2) and 17 alpha-OHP occurs followed by a second surge in T after 48-96 h. The present investigation focuses on the effect of a single dose of hCG (3500 IU/m2 body surface) on testicular steroid production in 12 boys aged 13 months to 12 yr. Plasma hCG, 17 alpha-OHP, androstenedione (A), T, dihydrotestosterone, and E2 were measured basally and 2, 4, 24, 48, 72, and 96 h after hCG administration. Plasma hCG was measured using a double antibody RIA technique and steroids by RIA after celite chromatography. The results show that hCG peaked 2 h after administration of the hormone and high levels persisted for up until 72 h. Plasma T and dihydrotestosterone increased after 48 h and remained significantly high for another 48 h; 17 alpha-OHP, A, and E2 did not change. These findings show that hCG stimulation in prepubertal boys induces significant production of T without affecting the precursors or aromatization product, in contrast to observation in the adult man, where 17 alpha-OHP, A, and E2 increase significantly. A response comparable to that observed in children has been recorded in adult males with hypogonadotropic hypogonadism.
D000375:Aging; D000735:Androstenedione / Q000097:blood; D002648:Child; D002675:Child, Preschool; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D013196:Dihydrotestosterone / Q000097:blood; D004958:Estradiol / Q000097:blood; D012739:Gonadal Steroid Hormones / Q000097:blood*; D006801:Humans; D006908:Hydroxyprogesterones / Q000097:blood; D007223:Infant; D008297:Male; D013737:Testis / Q000187:drug effects; D013739:Testosterone / Q000097:blood
D006063:Chorionic Gonadotropin; D012739:Gonadal Steroid Hormones; D006908:Hydroxyprogesterones; D013196:Dihydrotestosterone; D013739:Testosterone; D000735:Androstenedione; D004958:Estradiol