Effects of peptides cleaved from human fibrinogen by plasmin on rabbit kidney cells in culture.
Low molecular weight fibrinogen degradation products (LMW-FDP) containing a mixture of dialysable peptides cleaved from human fibrinogen by plasmin are cytotoxic to an established line of rabbit kidney cells and to primary cultures of rabbit kidney cells. The presence of LMW-FDP in a concentration of 50 micrograms/ml during the cell cultivation caused a considerable release of 51Cr from prelabelled cells and inhibited 3H-thymidine and 86Rb uptake. Among three isolated peptides of established primary structure only one, 6D: Ser-Gln-Leu-Gln-Lys-Val-Pro-Pro-Glu-Trp-Lys, induced a significant effect, i.e. it enhanced 3H-thymidine incorporation. Two others, 6A: Ala-Arg-Pro-Ala-Lys and 6E: Thr-Ser-Glu-Val-Lys, did not influence the examined parameters. Hence other components of LMW-FDP must be assumed to be responsible for the cytotoxic effect on kidney cell cultures.
D000818:Animals; D002478:Cells, Cultured; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis / Q000494:pharmacology*; D007668:Kidney / Q000378:metabolism*; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis / Q000494:pharmacology; D011817:Rabbits; D011868:Radioisotopes; D012413:Rubidium / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D005338:Fibrin Fibrinogen Degradation Products; D010455:Peptides; D011868:Radioisotopes; D012413:Rubidium; D013936:Thymidine