Characterization of oligosaccharide units of p-N-collagen type III from dermatosparactic bovine skin.
p-N-collagen type III was extracted from dermatosparactic and normal fetal bovine skin and purified by ion-exchange chromatography using DEAE- and CM-cellulose. Asparagine-linked sugar chains were fractionated by high voltage paper chromatography from the products obtained after hydrazinolysis and reduction with NaB3H4. These oligosaccharides composed of neutral and acidic components are mannose-containing oligosugars of the complex type. Their abundance is much higher in dermatosparactic p-N-collagen type III.
D000818:Animals; D001216:Asparagine; D002240:Carbohydrate Sequence; D002417:Cattle; D003094:Collagen / Q000302:isolation & purification*; D003095:Collagen Diseases / Q000378:metabolism*; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis*; D012794:Sialic Acids / Q000032:analysis; D012867:Skin / Q000032:analysis*; D012871:Skin Diseases / Q000378:metabolism*
D009844:Oligosaccharides; D012794:Sialic Acids; D001216:Asparagine; D003094:Collagen