Intralipid and free plasmatic tryptophan in vitro.
In an attempt to investigate the role of the lipidic emulsion Intralipid in the development of metabolic encephalopathy in a patient showing high free tryptophan levels, the relationship between lipidic emulsion and free tryptophan was examined in in vitro experiments. The addition of intralipid to normal serum produces an immediate increase in non-esterified fatty acids and a parallel rise in free tryptophan. Moreover, when serum with intralipid is incubated at 37 degrees C, the lipases release new non-esterified fatty acids and the free tryptophan increases proportionally. The non-esterified fatty acid content of intralipid was found to be 12 +/- 2 mEq X 1(-1). An inverse correlation was seen between free tryptophan and different serum albumin concentrations. It is concluded that intralipid causes an increase in free tryptophan levels. It is known that in vivo free tryptophan modulates 5-hydroxytryptamine synthesis and thus may be considered a possible causal agent for encephalopathy.
D001663:Bilirubin / Q000097:blood; D003128:Coma / Q000139:chemically induced / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005217:Fat Emulsions, Intravenous / Q000009:adverse effects / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D006801:Humans; D012709:Serum Albumin / Q000032:analysis; D014364:Tryptophan / Q000097:blood*
D005217:Fat Emulsions, Intravenous; D005230:Fatty Acids, Nonesterified; D012709:Serum Albumin; D014364:Tryptophan; D001663:Bilirubin