Characterization of intracellular inclusions formed by Pseudomonas oleovorans during growth on octane.
The growth of Pseudomonas oleovorans on n-octane was characterized by the formation of intracellular structures. These inclusions were isolated and characterized. Morphologically, they resembled the poly-beta-hydroxybutyrate granules found in Bacillus cereus, as shown by freeze-fracture electron microscopy. The elemental analysis of isolated granules showed, however, that they do not contain poly-beta-hydroxybutyric acid. Instead, the analysis was consistent with a C8 polyester, which interpretation was supported by the fatty acid analysis of hydrolyzed granules. From the evidence presented here, we conclude that P. oleovorans forms poly-beta-hydroxyoctanoate granules when grown on n-octane.
D002210:Caprylates / Q000032:analysis; D002458:Cell Fractionation; D003594:Cytoplasmic Granules / Q000032:analysis / Q000648:ultrastructure*; D005614:Freeze Fracturing; D008854:Microscopy, Electron; D009795:Octanes / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism / Q000648:ultrastructure*
D002210:Caprylates; D009795:Octanes; C026728:octane