Effect of biotin on alkaloid production during submerged cultivation of Claviceps sp. strain SD-58.
Addition of biotin to culture medium NL-406 significantly increased alkaloid yield during submerged cultivation of Claviceps sp. strain SD-58. Alkaloid yield was further enhanced by incorporating leucine in biotin-supplemented culture medium. Increased alkaloid production was associated with an increase in the lipid content of cells and in the number of chlamydospores. Biotin deficiency caused a reduction in alkaloid yield and a parallel decrease in lipid content and chlamydospore numbers.
D000470:Alkaloids / Q000096:biosynthesis*; D001360:Avidin / Q000494:pharmacology; D001710:Biotin / Q000494:pharmacology*; D002967:Claviceps / Q000378:metabolism*; D003470:Culture Media; D007700:Kinetics; D007930:Leucine / Q000494:pharmacology
D000470:Alkaloids; D003470:Culture Media; D001360:Avidin; D001710:Biotin; D007930:Leucine