A simple assay to study protein-mediated lipid exchange by fluorescence polarization.
We investigated the protein-mediated phospholipid transfer between small vesicles by fluorescence polarization measurements with diphenylhexatriene as optical probe. Thermotropic-phase-transition curves were taken after mixing two vesicle preparations of lipids exhibiting different gel-to-liquid phase transitions. From the heights of each phase-transition step we were able to follow the lipid transfer process without separating the two vesicle preparations.
D002352:Carrier Proteins / Q000032:analysis*; D004161:Diphenylhexatriene; D005454:Fluorescence Polarization / Q000379:methods; D008051:Lipid Bilayers / Q000032:analysis*; D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids / Q000032:analysis*; D013997:Time Factors
D002352:Carrier Proteins; D008051:Lipid Bilayers; D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids; D004161:Diphenylhexatriene