title: Protein secretion by the principal cells of mouse epididymis evidenced by in vitro incorporation of tritiated leucine.
abstract: The in vitro synthesis and secretion of newly formed proteins were investigated in the principal cells of small pieces of the head and body of mouse epididymis inserted into a modified Rose chamber. Our ultrastructural observations revealed that the cells resumed their normal ultrastructural appearance only after 3 h. Therefore, after preliminary 3-hour culture, the pieces were incubated with tritiated leucine at intervals ranging from 5 min to 6 h. The incorporation of radioactive leucine measured by scintillation counting demonstrated that each of the anatomical segments studied secreted a significant amount of proteins into the culture medium.
mesh_terms: D000818:Animals; D046508:Culture Techniques; D003593:Cytoplasm / Q000648:ultrastructure; D004822:Epididymis / Q000378:metabolism* / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure; D007930:Leucine / Q000378:metabolism*; D008297:Male; D051379:Mice; D011506:Proteins / Q000378:metabolism*; D014316:Tritium
chemical_list: D011506:Proteins; D014316:Tritium; D007930:Leucine