High performance liquid chromatographic determination of malondialdehyde in vegetable oils.
A simple high performance liquid chromatographic (HPLC) method has been developed for determining malondialdehyde (MDA) in vegetable oils. MDA was reacted with dansyl hydrazine in an acidic medium, and the product, 1-dansyl-pyrazole, was determined by HPLC, using a Zorbax sil column with mixed mobile phase of n-hexane-methylene chloride. MDA can be determined as 1-dansyl-pyrazole by fluorometric detection at a level of 0.01 ppm in vegetable oils.
D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds; D004041:Dietary Fats / Q000032:analysis; D005456:Fluorescent Dyes; D008314:Malonates / Q000032:analysis*; D008315:Malondialdehyde / Q000032:analysis*; D009821:Oils / Q000032:analysis*; D010545:Peroxides / Q000032:analysis; D010945:Plants, Edible; D011720:Pyrazoles; D013050:Spectrometry, Fluorescence
C037944:1-dansylpyrazole; D003619:Dansyl Compounds; D004041:Dietary Fats; D005456:Fluorescent Dyes; D008314:Malonates; D009821:Oils; D010545:Peroxides; D011720:Pyrazoles; D008315:Malondialdehyde