A method for the determination of octa- and nonachloro-2-phenoxyphenols in chicken tissue.
A gas-liquid chromatographic method was developed capable of determining octa- and nonachloro-2-phenoxyphenols in chicken liver or muscle at 0.25 ppb and fat at 2.5 ppb. The method involves extraction with acidified acetone:hexane, cleanup with concentrated H2SO4 and Florisil column chromatography, methylation with diazomethane, and quantitation by capillary column gas-liquid chromatography with electron capture detection. Fortification of liver and muscle at 0.25 or 0.5 ppb and fat at 2.5 or 5.0 ppb and subsequent analysis yielded recoveries averaging 91% for octa- and 97% for nonachlorophenoxyphenol.
D000273:Adipose Tissue / Q000032:analysis; D000818:Animals; D002645:Chickens; D002849:Chromatography, Gas / Q000379:methods; D055768:Halogenated Diphenyl Ethers; D007202:Indicators and Reagents; D008099:Liver / Q000032:analysis*; D008832:Microchemistry; D009132:Muscles / Q000032:analysis*; D010647:Phenyl Ethers / Q000032:analysis*
D055768:Halogenated Diphenyl Ethers; D007202:Indicators and Reagents; D010647:Phenyl Ethers; C024879:nonachloropredioxin; C038168:octachloro-2-phenoxyphenol