[Comparative study of the biological properties of influenza virus preparations purified by centrifugation and chromatography].
A comparative study of chromatography and centrifugation as applied to purification of 2 influenza virus strains with different antigenic structure (H0N1 and H2N2) and of some biological properties of the resulting preparations was carried out. Certain strain-specific differences manifested in their chromatographic behaviour and in the degree of purification of virions from the allantoic fluid proteins were found. Some quantitative differences observed in the capacity of the resulting virus preparations to infect chick embryos and primary and continuous cells are insignificant and cannot be used for judgement of advantages of one or the other of the methods used for influenza virus purification.
D000818:Animals; D002500:Centrifugation, Isopycnic / Q000379:methods; D002642:Chick Embryo; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002850:Chromatography, Gel; D009980:Influenza A virus / Q000302:isolation & purification*; D013045:Species Specificity; D014461:Ultracentrifugation / Q000379:methods
Sravnitel'noe izuchenie biologicheskikh svoĭstv preparatov virusa grippa, ochishchennykh metodami tsentrifugirovaniia i khromatografii.