title: Characteristics and determinants of osmotic lysis in chromaffin granules.
abstract: (1) Using isolated bovine chromaffin granules, we demonstrate that osmotic lysis is not a random process and establish the osmotic pressure dependence of osmotic lysis in chromaffin granules, the so-called osmotic fragility curve. (2) We show by measuring the release of constituents of the granule core and correlating these with changes in spectroscopic parameters (turbidity and endogenous catecholamine fluorescence), that the latter can be safely used to measure lysis. (3) Within a particular granule population, noradrenaline granules lyse at higher osmolarities than adrenaline granules, suggesting a higher core osmolarity of the noradrenaline granules. (4) The size distribution of chromaffin granules as a function of lysis was determined by the use of whole mount electron microscopy. It is shown that the mean size of chromaffin granules decreases as a function of lysis. (5) On the basis of theoretical considerations three alternative models of the sequence of osmotic lysis in chromaffin granules are proposed. The experimental results best support a model which postulates that during partial osmotic lysis, granule membranes reseal into smaller vesicles after graded release of contents. The osmotic fragility would represent several cycles of lysis and resealing and would not be a reflection of the distribution of osmotic pressures in the granule population.
mesh_terms: D000818:Animals; D002417:Cattle; D002837:Chromaffin Granules / Q000648:ultrastructure*; D002838:Chromaffin System / Q000648:ultrastructure*; D004837:Epinephrine / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D009996:Osmotic Fragility; D009997:Osmotic Pressure; D013050:Spectrometry, Fluorescence; D013395:Sucrose
chemical_list: D013395:Sucrose; D009638:Norepinephrine; D004837:Epinephrine