Pseudo-on-line fast response microvessel dimensions video graphic recorder with electrical signal output.
The present article describes a new method of microvessel dimensions measurement in which a writing oscilloscope is used to continuously and graphically record the microvessel video signal from television microscopy at a fast rate of 50 records per second. Dimensions of interest, such as the microvessel red blood cell flux diameter, are then easily marked out manually from the graphic records and a dynamic electrical signal proportional to the dimensions is generated. The signal is then recorded on one channel of a multichannel voltage recorder and is synchronised with other experimental signals which have previously been recorded on-line during the experiment. The result is that the dimension signal appears to have been recorded on-line during the experiment as well. This is desirable for electronic signal correlation and processing. This method is useful when poor experimental conditions, commonly encountered, make automatic recording of microvessel dimension unsatisfactory and manual inspection and processing become necessary.
D000818:Animals; D001808:Blood Vessels / Q000033:anatomy & histology*; D004912:Erythrocytes / Q000166:cytology; D008297:Male; D008833:Microcirculation / Q000033:anatomy & histology*; D010784:Photomicrography / Q000295:instrumentation / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains