Action spectra for ultraviolet photosensitized killing of mammalian cells by misonidazole and para-nitroacetophenone.
V-79 Chinese hamster fibroblasts in monolayer culture were exposed to ultraviolet radiation at 313, 334, 365, 380, and 405 nm in the presence of either misonidazole or para-nitroacetophenone, drugs which act as both photosensitizers and radiosensitizers of cell killing. Survival was measured by a colony-forming assay. The resulting action spectra for cell death photosensitized by the drugs (the reciprocals of the exposures required at each wavelength to reduce cell survival to a given level) closely match their absorption spectra over a range of three orders of magnitude. These results demonstrate that cells can be killed upon excitation of misonidazole or para-nitroacetophenone in the absence of any other types of energy deposition or biomolecular damage.
D000098:Acetophenones / Q000494:pharmacology* / Q000528:radiation effects; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D004307:Dose-Response Relationship, Radiation; D005609:Free Radicals; D008920:Misonidazole / Q000494:pharmacology* / Q000528:radiation effects; D009593:Nitroimidazoles / Q000494:pharmacology*; D010777:Photochemistry; D010782:Photolysis; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays
D000098:Acetophenones; D005609:Free Radicals; D009593:Nitroimidazoles; C001972:4-nitroacetophenone; D008920:Misonidazole