Toxicity of some charged lipids used in liposome preparations.
The inhibition of 3H-thymidine incorporation into DNA of L1210 cells in culture by liposomes was used as an index of liposome toxicity. Inhibition of 3H-thymidine incorporation appeared to be dose dependent for some lipid compositions tested. The commonly used neutral lipids, phosphatidylcholine and cholesterol did not appear to inhibit 3H-thymidine incorporation. Phosphatidic acid, an adjunct for preparing anionic liposomes, appeared to be non-toxic compared to phosphatidylserine and dicetylphosphate (alternative adjuncts for preparing anionic liposomes). Stearylamine, a synthetic lipid which continued to dominate the preparation of cationic liposome was the most toxic of the lipids tested.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002784:Cholesterol / Q000633:toxicity; D004247:DNA / Q000378:metabolism; D007939:Leukemia L1210 / Q000378:metabolism; D008055:Lipids / Q000633:toxicity*; D008081:Liposomes / Q000633:toxicity*; D051379:Mice; D010712:Phosphatidic Acids / Q000633:toxicity; D010713:Phosphatidylcholines / Q000633:toxicity; D010718:Phosphatidylserines / Q000633:toxicity; D013936:Thymidine / Q000378:metabolism
D008055:Lipids; D008081:Liposomes; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D004247:DNA; D002784:Cholesterol; D013936:Thymidine