Enzymatic and pharmacokinetic studies on the metabolism of branched chain alpha-keto acids in the rat.
Michaelis-constants and enzyme activities for dehydrogenation and transamination of the three branched chain alpha-keto acids in liver, kidney, skeletal muscle, and brain of rats are reported. After oral load only 11-22% of the keto acids pass the liver unchanged. Blood levels in pharmacokinetic and absorption studies are related to the Michaelis-constants. At the low keto-acid concentrations after oral application, dehydrogenation in the non-hepatic tissues is supposed to prevail over transamination. Data on feed efficiency of branched chain alpha-keto acids reported in the literature support this view. The chance for transamination is better after intravenous administration. The transferability of our data to humans, and various factors influencing the efficiency of branched chain alpha-keto acids are discussed in connection with data reported in the literature.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002474:Cell-Free System; D045782:Hemiterpenes; D007651:Keto Acids / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism; D008931:Mitochondria, Muscle / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D000637:Transaminases / Q000378:metabolism
D045782:Hemiterpenes; D007651:Keto Acids; C001505:alpha-ketoisovalerate; C013082:alpha-ketoisocaproic acid; D010088:Oxidoreductases; D000637:Transaminases