[Study of the ion transfer in membrane vesicles by ion exchange gel chromatography and ultrafiltration].
A method involving fast ion-exchange gel chromatography and filtration through ultrafilters is suggested for studying ion transport in membrane vesicles from bovine brain gray matter. The method permits separating quantitatively the membrane vesicles from the external radioactive label and studying kinetics of ion transfer. The label transfer is characterized by the presence of fast and slow phases and cannot be described by one exponential curve.
D000818:Animals; D001692:Biological Transport; D002417:Cattle; D002850:Chromatography, Gel / Q000379:methods; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism*; D007553:Isotope Labeling; D007700:Kinetics; D008861:Microsomes / Q000378:metabolism*; D010487:Periaqueductal Gray / Q000378:metabolism*; D012413:Rubidium / Q000378:metabolism; D014462:Ultrafiltration
D012413:Rubidium
Izuchenie perenosa ionov v membrannykh vezikulakh s pomoshch'iu ionobmennoĭ gel'-khromatografii i ul'trafil'tratov.