title: A simple cell disrupter designed for small cells with relatively large nuclei.
abstract: A simple cell disrupter that is particularly suitable for breaking small cells with relatively large nuclei is described. Cells are disrupted by the shearing forces set up as they are pushed by a positive N2 pressure through a 0.8- to 1.5-micrometer stainless-steel filter. The total procedure takes only a few minutes, it is highly reproducible, and the yield is good. The cell homogenate obtained is a good starting source for the isolation of plasma membranes, intracellular organelles, and nuclei.
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000295:instrumentation*; D002462:Cell Membrane; D002467:Cell Nucleus; D009836:Oligodendroglia / Q000032:analysis; D013347:Subcellular Fractions