title: Fluoride, hydrogen, and formate activate ribulosebisphosphate carboxylase formation in Alcaligenes eutrophus.
abstract: Alcaligenes eutrophus formed ribulosebisphosphate carboxylase (RuBPCase; EC 4.1.1.39) when grown on fructose. Addition of sodium fluoride (NaF) to fructose minimal medium resulted in a slightly decreased growth rate and a rapid fivefold increase in RuBPCase specific activity. With citrate, a glucogenic carbon source, RuBPCase was also formed, However, addition of NaF to cells growing on citrate resulted in a 50% decrease in RuBPCase specific activity. Among the enzymes of fructose catabolism, NaF (10 mM) inhibited enolase in vitro by 98% and gluconate 6-phosphate dehydratase by 87%. Inhibition of the dehydratase by NaF was insignificant in vivo, as determined with a mutant defective in phosphoglycerate mutase activity. Growth of this mutant on fructose was not inhibited by NaF, and only a minor increase in RuBPCase activity was observed. From these results, we concluded that the product of the enolase reaction, phosphoenolpyruvate, played a role in RuBPCase formation. Addition of H2 or formate to the wild type growing on fructose or citrate did not affect the growth rate but resulted in rapid formation of RuBPCase activity. Mutants impaired in H2 metabolism formed RuBPCase at a low rate during growth on fructose plus H2 but at a high rate on formate. Apparently, additional reductant from H2 or formate metabolism induced RuBPCase formation in A. eutrophus.
mesh_terms: D000421:Alcaligenes / Q000201:enzymology* / Q000254:growth & development; D002262:Carboxy-Lyases / Q000096:biosynthesis*; D002951:Citrates / Q000378:metabolism; D019343:Citric Acid; D004790:Enzyme Induction; D005459:Fluorides / Q000494:pharmacology*; D005561:Formates / Q000378:metabolism / Q000494:pharmacology*; D005632:Fructose / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism / Q000494:pharmacology*; D012273:Ribulose-Bisphosphate Carboxylase / Q000096:biosynthesis*; D012969:Sodium Fluoride / Q000494:pharmacology*
chemical_list: D002951:Citrates; D005561:Formates; C030544:formic acid; D019343:Citric Acid; D005632:Fructose; D006859:Hydrogen; D012969:Sodium Fluoride; D002262:Carboxy-Lyases; D012273:Ribulose-Bisphosphate Carboxylase; D005459:Fluorides