Characterization of the cytotoxin produced by macrophages in response to dengue virus-induced cytotoxic factor.
We have observed earlier that dengue type 2 virus-induced cytotoxic factor (CF) induces macrophages to produce a cytotoxin (CF2) which kills mainly the macrophages, some of the T lymphocytes and has no effect on B-lymphocytes of normal mouse spleen. The findings of the present study show that CF2 is heat-labile, trypsin-sensitive and unstable at acid and alkaline pH. It is a low molecular weight product as it is dialysable, non-sedimentable on ultracentrifugation at 103,500 g for 3 h and passes through 0.22 micron Millipore filter. It is adsorbed onto the target normal mouse spleen cells. The properties of CF and CF2 have been compared.
D000327:Adsorption; D000818:Animals; D003602:Cytotoxicity, Immunologic; D003603:Cytotoxins / Q000096:biosynthesis / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology; D003956:Dialysis; D005374:Filtration; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D013154:Spleen / Q000276:immunology; D014357:Trypsin; D014461:Ultracentrifugation
D003603:Cytotoxins; D014357:Trypsin