[Analysis of the specific binding of 3H-estradiol by the uterus of androgen-sterile rats using biochemical and autoradiographic methods].
Experiments were performed on neonatal rats, given different doses of androgen testosterone propionate (25-50-100 micrograms). A decrease was seen in the concentration of estrogen receptors in cytosol and nuclear fractions of the uterine tissue comparatively to those of the normal rats regardless of the estrous cycle stage. These changes were less pronounced in rats with the open vagina, given testosterone (25 micrograms). The reduction of specific estradiol-3H binding by different fractions of the uterine tissue occurs in androgen-sterile rats under the action of testosterone (50 to 100 micrograms) due to epithelial cells, as it was shown by autoradiography. The role of this effect for infertility of the animals under study is discussed.
D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D001665:Binding Sites / Q000187:drug effects; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007247:Infertility, Female / Q000139:chemically induced / Q000378:metabolism*; D051381:Rats; D011959:Receptors, Estradiol; D011960:Receptors, Estrogen / Q000187:drug effects / Q000378:metabolism; D013739:Testosterone / Q000008:administration & dosage*; D014316:Tritium; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D011959:Receptors, Estradiol; D011960:Receptors, Estrogen; D014316:Tritium; D013739:Testosterone; D004958:Estradiol
Analiz spetsificheskogo sviazyvaniia 3H-éstradiola matkoĭ androgensteril'nykh krys biokhimicheskim i avtoradiograficheskim metodami.