Possible amino acid repetitive sequences in the proline-rich glycoprotein of human parotid saliva.
The amino acid sequence of the proline-rich glycoprotein from human parotid saliva was studied using different preparations: native glycoprotein, tryptic glycopeptides, and their chemically deglycosylated homologous derivatives. The results indicate very similar structural features of the proline-rich glycoprotein and the tryptic glycopeptides and suggest that the peptide backbone of the glycoprotein comprises several repetitive domains containing one carbohydrate-peptide linkage and a proline-rich sequence. These data are in fair agreement with a preceding circular dichroism study (Aubert, J.P., Porchet, N., Boersma, A., Loucheux-Lefevbre, M.H. and Degand, P. (1982) Biochim. Biophys. Acta 704, 361-365).
D000595:Amino Acid Sequence; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D010306:Parotid Gland / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis; D011392:Proline / Q000032:analysis*; D012463:Saliva / Q000032:analysis*; D014357:Trypsin / Q000032:analysis
D002241:Carbohydrates; D006023:Glycoproteins; D010446:Peptide Fragments; D011392:Proline; D014357:Trypsin