A sensitive assay for creatine kinase in serum samples dried on paper: enhanced thermal stability of the dried enzyme.
A new bioluminescent creatine kinase (CK) assay using purified luciferase was used to analyse CK activity in serum samples dried on filter paper. Enzyme activity was preserved for over 1 wk on paper stored at room temperature. At 60 degrees C, CK activity in liquid serum samples was rapidly inactivated, but the activity of enzyme stored on paper was preserved for at least 2 days.
D004796:Clinical Enzyme Tests / Q000379:methods*; D003402:Creatine Kinase / Q000097:blood*; D006358:Hot Temperature; D006801:Humans; D008156:Luciferases / Q000494:pharmacology; D009203:Myocardial Infarction / Q000175:diagnosis
D008156:Luciferases; D003402:Creatine Kinase