Comparative quality control of 99mTc-Pyrophosphate and 99mTc-diphosphonate radiopharmaceuticals.
The results of analysis of 99mTc-Pyrophosphate (99mPyP), taken as a representative of the group of compounds having an organic P-O-P bond, and of the three diphosphonate compounds: methylene diphosphonate (MDP), 2,3-dicarboxy propane diphosphonate (DPD) and ethane-1-hydroxy-1, 1-diphosphonate (EHDP), which differ in their chemical structure, are shown. Also, some physicochemical parameters such as chloroform-water apparent partition coefficient, the osmotic pressure and pH values in final preparations were studied. The radiochemical purity of these radiopharmaceuticals was determined by the two methods: Sephadex chromatography for separation of 99mTc-hydrolysate and TLC on silica gel with 85% methanol for the determination of free 99mTcO-4. The yield of labelling for both methods was over 90%. Also, pharmacokinetic parameters such as binding to the plasma proteins and to erythrocytes were determined. 99mTc-PyP binding to plasma proteins was higher than the binding of diphosphonate compounds. The quantitative distribution of preparations was determined in experimental animals.
D000818:Animals; D004164:Diphosphonates / Q000378:metabolism / Q000592:standards*; D007700:Kinetics; D011122:Polyphosphates / Q000592:standards*; D011485:Protein Binding; D011786:Quality Control; D051381:Rats; D013667:Technetium / Q000378:metabolism / Q000592:standards*; D017556:Technetium Compounds; D016698:Technetium Tc 99m Pyrophosphate; D014003:Tin Polyphosphates / Q000378:metabolism / Q000592:standards*
D004164:Diphosphonates; D011122:Polyphosphates; D017556:Technetium Compounds; D014003:Tin Polyphosphates; C017742:technetium Tc 99m stannous pyrophosphate; D016698:Technetium Tc 99m Pyrophosphate; D013667:Technetium; C029045:technetium Tc 99m diphosphonate