A rapid method for removal of detergents from protein solution.
A simple and rapid technique is described for the removal of Triton X-100, deoxycholate, and cholate from protein solutions. The method involves a 2-min centrifugation of the sample on a Bio-Beads SM-2 bed prepared in a microcentrifuge tube and is suitable for multiple assays of 0.05- to 0.45-ml samples. Another advantage of this method is the high recovery of proteins without dilution of the sample.
D019826:Cholic Acid; D002793:Cholic Acids / Q000302:isolation & purification; D003840:Deoxycholic Acid / Q000302:isolation & purification; D003902:Detergents / Q000302:isolation & purification*; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000302:isolation & purification; D011506:Proteins; D012996:Solutions; D013501:Surface-Active Agents / Q000302:isolation & purification*; D014461:Ultracentrifugation
D002793:Cholic Acids; D003902:Detergents; D011506:Proteins; D012996:Solutions; D013501:Surface-Active Agents; D003840:Deoxycholic Acid; D011092:Polyethylene Glycols; D017830:Octoxynol; D019826:Cholic Acid