A method for distinguishing Cu,Zn- and Mn-containing superoxide dismutases.
A procedure has been developed to distinguish between the two forms of eukaryotic superoxide dismutases using a common activity assay. Treatment of cellular fractions with 2% sodium dodecyl sulfate at 37 degrees C for 30 min selectively inactivates the mitochondrial, manganese-containing variant without affecting the cytosolic copper, zinc-superoxide dismutase. After removing excess sodium dodecyl sulfate by precipitation with potassium chloride, the supernate is assayed using the xanthine oxidase-cytochrome c method.
D000818:Animals; D002458:Cell Fractionation; D003300:Copper; D003600:Cytosol / Q000201:enzymology*; D008297:Male; D008345:Manganese; D008930:Mitochondria, Liver / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D013997:Time Factors; D015032:Zinc
D012967:Sodium Dodecyl Sulfate; D008345:Manganese; D003300:Copper; D013482:Superoxide Dismutase; D015032:Zinc