Regulation of rat pineal hydroxyindole-O-methyltransferase in neonatal and adult rats.
The relative importance of neural, and some nonneural, mechanisms in the control of pineal hydroxyindole-O-methyltransferase (HIOMT) activity during development and in the adult rat was studied. In neonatal rats, guanethidine-treatment, bilateral superior cervical ganglionectomy (SCGX), or exposure to constant light did not prevent the initial appearance of HIOMT activity, indicating that neural stimulation of the gland is not essential for the development of HIOMT activity. In adult rats, decentralization or removal of the SCG led to a slow fall in HIOMT activity, to about 30% of control activity, indicating that the enzyme is largely under neural control. Additionally, adrenalectomy or hypophysectomy had no effect on HIOMT activity, refuting the suggestion that adrenal and/or gonadal steroids are of major importance in the regulation of this enzyme. The fall in activity of the enzyme after SCGX or exposure to constant light probably does not represent a shift in the Km of the enzyme nor the selective disappearance of a distinct molecular species. Similar changes in HIOMT activity and cyclic GMP responsiveness occur in response to alterations in the length of the daily dark period, adding further evidence to our earlier speculation that there may be a functional relationship between these two.
D000121:Acetylserotonin O-Methyltransferase / Q000378:metabolism*; D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D007016:Hypophysectomy; D007700:Kinetics; D008297:Male; D008780:Methyltransferases / Q000378:metabolism*; D010870:Pineal Gland / Q000201:enzymology* / Q000254:growth & development; D051381:Rats; D011919:Rats, Inbred Strains
D008780:Methyltransferases; D000121:Acetylserotonin O-Methyltransferase