Procaryotic genomic DNA inhibits mammalian cell transformation.
Ltk- mouse cells were transformed to thymidine kinase prototrophy in the presence of carrier DNAs isolated from different organisms. Procaryotic genomic and phage DNA was consistently less effective as a carrier than was eucaryotic DNA. Mixing experiments indicate that DNA of procaryotic origin inhibits mammalian cell transformation.
D000818:Animals; D002417:Cattle; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics*; D004269:DNA, Bacterial / Q000494:pharmacology*; D004279:DNA, Viral / Q000494:pharmacology*; D005057:Eukaryotic Cells; D006801:Humans; D051379:Mice; D011387:Prokaryotic Cells; D012474:Salmon; D013937:Thymidine Kinase / Q000235:genetics; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D004279:DNA, Viral; D004247:DNA; D013937:Thymidine Kinase