Subcellular distribution in Xenopus laevis oocytes of a microinjected poly(A)-binding protein from Artemia salina gastrulae.
The poly(A)-binding protein P38 of non-polysomal mRNP from Artemia salina gastrulae was labelled by reductive methylation and microinjected into the cytoplasm of Xenopus laevis oocytes. The labelled protein has a half-life of approximately 20 h and accumulated in the nucleus of the oocyte. The kinetics of accumulation reached a plateau at about 15 h after microinjection. P38 accumulates in the nucleus to a final concentration 3.15 times higher than that reached by free diffusion. This fact suggests that P38, a cytoplasmic poly(A)-binding protein, might also play some role in the nucleus of the cell.
D000818:Animals; D001156:Artemia / Q000196:embryology; D002352:Carrier Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D039102:Poly(A)-Binding Proteins; D013347:Subcellular Fractions / Q000378:metabolism*; D014982:Xenopus laevis / Q000378:metabolism
D002352:Carrier Proteins; D039102:Poly(A)-Binding Proteins