Aging of the erythrocyte. 23. Changes in the permeation of spin-labeled electrolytes.
The transport of spin-labeled anions and cations was studied in bovine erythrocyte fractions of various ages. Permeation of a spin-labeled anion, 3-carboxy-2,2,5,5-tetramethylpyrrolidine-1-oxyl into the cells was found to be significantly slower in older cells, suggesting a functional impairment of the band 3 protein of the erythrocyte membrane during red cell aging in vivo.
D000818:Animals; D001692:Biological Transport; D002417:Cattle; D003497:Cyclic N-Oxides / Q000378:metabolism; D004573:Electrolytes / Q000378:metabolism*; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000378:metabolism*; D013113:Spin Labels / Q000378:metabolism
D003497:Cyclic N-Oxides; D004573:Electrolytes; D013113:Spin Labels; C032289:2,2,5,5-tetramethyl-1-pyrrolidinyloxy-3-carboxylic acid; C017743:tempocholine