Modified method for production and purification of Staphylococcus aureus enterotoxin B.
A medium containing 4% bio-trypcase and 1% yeast extract was used for the production of Staphylococcus aureus enterotoxin B. The yield obtained was estimated at 200 micrograms of enterotoxin per ml of S. aureus S-6 culture supernatant. The purification method involves chromatography on Biorex 70 resin, isoelectric focusing, and gel filtration on Sephadex G-100. The purified enterotoxin (isoionic point, pH 8.55) was shown to be homogenous protein with a molecular weight of 29,000 when tested by gel electrophoresis.
D002850:Chromatography, Gel; D004768:Enterotoxins / Q000096:biosynthesis* / Q000302:isolation & purification; D007525:Isoelectric Focusing; D013211:Staphylococcus aureus / Q000378:metabolism*
D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal