Low-temperature cryopreservation of BB rat embryos of spontaneously diabetic rats.
Cryopreservation of BB rat embryos would ensure perpetuation, at greatly reduced cost, of this model of nonobese, insulin-dependent, juvenile-onset diabetes in man. Immature prediabetic female BB rats were ovulated hormonally. The embryos were removed at the 8- to 16 cell and blastocyst stages and cryopreserved, and after thawing were assayed for viability in vitro or transplanted into pseudopregnant foster mothers of a nondiabetic strain. The highest rate of viability in vitro (100%) and development into normal fetuses (31%) was obtained with blastocysts transplanted into day-4 foster mothers. The results indicate the feasibility of low-temperature banking of this clinically important strain of rats.
D000818:Animals; D003921:Diabetes Mellitus, Experimental; D003922:Diabetes Mellitus, Type 1; D004624:Embryo Transfer; D004622:Embryo, Mammalian; D005260:Female; D005615:Freezing; D010062:Ovulation Induction; D011247:Pregnancy; D011254:Pregnancy in Diabetics; D011309:Preservation, Biological / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains