Coat protein conformation in M13 filaments, I-forms and spheroids.
Circular dichroism studies of the filamentous coliphage M13 were carried out to determine conformational changes in the major capsid protein (the B protein) that occur during contraction of the filaments to I-forms and spheroids. The alpha-helicity of the B protein is somewhat lower in the I-forms than in filaments and much lower in spheroids. This conformational change may explain the increased detergent and lipid solubility of both I forms and spheroids relative to filaments.
D002213:Capsid / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D003090:Coliphages / Q000032:analysis* / Q000648:ultrastructure; D003902:Detergents; D008055:Lipids; D011487:Protein Conformation; D012995:Solubility; D014764:Viral Proteins / Q000302:isolation & purification*
D003902:Detergents; D008055:Lipids; D014764:Viral Proteins