The effect of the male contraceptive agent Gossypol on human lymphocytes in vitro: traditional chromosome breakage, micronuclei, sister chromatid exchange, and cell kinetics.
The male antifertility agent Gossypol did not affect the level of traditional chromosome breakage or number of micronuclei in 66-hour lymphocyte cultures at concentrations up to 40 micrograms/ml. It did increase the frequency of SCE slightly, although the inter-individual variation was greater than the increase resulting from Gossypol, and, even at the highest concentration (40 micrograms/ml), the SCE rate was still within the normal range. It also affected cell kinetics, reducing the mitotic index and the proportion of second and third metaphases after BUdR incorporation.
D000328:Adult; D002467:Cell Nucleus / Q000187:drug effects*; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D003434:Crossing Over, Genetic / Q000187:drug effects*; D006072:Gossypol / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D008938:Mitosis / Q000187:drug effects*; D010835:Phytohemagglutinins; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D010835:Phytohemagglutinins; D006072:Gossypol