Stabilization of beta-turn conformations in enkephalins. alpha-Aminoisobutyric acid analogs.
Stereochemical constraints have been introduced into the enkephalin backbone by substituting alpha-aminoisobutyryl (Aib) residues at positions 2 and 3, instead of Gly. 1H n.m.r. studies of Tyr-Aib-Gly-Phe-Met-NH2, Tyr-Aib-Aib-Phe-Met-NH2 and Tyr-Gly-Aib-Phe-Met-NH2 demonstrate the occurrence of folded, intramolecularly hydrogen bonded structures in organic solvents. Similar conformations are also favoured in the corresponding t-butyloxycarbonyl protected tetrapeptides, which lack the Tyr residue. A beta-turn centred at positions 2 and 3 is proposed for the Aib2-Gly3 analog. In the Gly2-Aib3 analog, the beta-turn has Aib3-Phe4 as the corner residues. The Aib2-Aib3 analog adopts a consecutive beta-turn or 3(10) helical conformation. High in vivo biological activity is observed for the Aib2 and Aib2-Aib3 analogs, while the Aib3 peptide is significantly less active.
D000621:Aminoisobutyric Acids; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D004745:Enkephalins / Q000494:pharmacology; D006860:Hydrogen Bonding; D051379:Mice; D011487:Protein Conformation; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
D000621:Aminoisobutyric Acids; D004745:Enkephalins; C100049:2-aminoisobutyric acid