A latex agglutination test for lectin binding.
Blood group A + H substance purified from hog gastric mucin was fixed to polystyrene particles by physical absorption to produce a lectin-agglutinable latex. Agglutination of this latex by soybean agglutinin, concanavalin A, and wheat germ agglutinin is specifically inhibited by the respective monosaccharide haptens and is not affected by known noninhibitory sugars. The sensitivity of the assay, a function of particle loading and latex concentration, approaches that of the hemagglutination test. NaCl, NaHCO3, NH4CO3, NH4-acetate, and Tris-HCl up to a concentration of 1 M do not interfere with the test. The latex suspension is stable for several months and can be stored in freeze-dried form.
D000017:ABO Blood-Group System; D000818:Animals; D006023:Glycoproteins; D007841:Latex Fixation Tests; D037102:Lectins / Q000032:analysis*; D011485:Protein Binding; D013552:Swine
D000017:ABO Blood-Group System; D006023:Glycoproteins; D037102:Lectins