[Manifold reflection infrared spectroscopy of the hair of mice with an experimental amino acid metabolism disease (L-acetidin-2-carboxylic acid incorporation instead of proline). A model for the use of infrared spectroscopy as a screening method for metabolic diseases].
8 mice were given L-acetidin-2-carboxylic acid orally over a period of 5 weeks. Another 8 mice served as control animals. Finally, the mice were sacrificed, their hair samples were hydrolized and applied to thin layer chromatography which revealed the incorporation of L-acetidin-2-carboxylic acid into mouse hair expressed by an additional spot. This experimental amino acid metabolic disorder served as a model for the application of infrared spectroscopy for screening hair in order to check metabolic disorders. As the multiinternal reflection-infrared spectra showed significant differences we suggest that infrared spectroscopy can be used as a noninvasive screening technique.
D000592:Amino Acid Metabolism, Inborn Errors / Q000175:diagnosis*; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002855:Chromatography, Thin Layer; D006197:Hair / Q000378:metabolism*; D051379:Mice; D011392:Proline / Q000031:analogs & derivatives* / Q000378:metabolism*; D013057:Spectrum Analysis / Q000379:methods*
D000596:Amino Acids; D011392:Proline
Vielfachreflexions-Infrarotspektroskopie an Haaren von Mäusen mit einer experimentellen Aminosäurestoff-wechselerkrankung (L-Acetidin-2-Carbonsäure-Einbau anstelle von Prolin). Ein Modell für die Anwendung der Infrarot-Spektroskopie als Screenings-methode für. Stoffwechselerkrankungen.