High-performance liquid chromatography of coproporphyrin isomers.
A reversed-phase system is described for the simultaneous isocratic separation of coproporphyrin I, II, III and IV isomers. The retention behaviour of coproporphyrin I and III is studied in detail. The method is suitable for both analytical and semi-preparative separation.
D000097:Acetonitriles; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003306:Coproporphyrins / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D011166:Porphyrins / Q000302:isolation & purification*
D000097:Acetonitriles; D002021:Buffers; D003306:Coproporphyrins; D011166:Porphyrins; C032159:acetonitrile