[Method of isolation of intact enterocytes from rat duodenal mucosa].
A procedure is suggested for obtaining intact enterocytes from rat duodenum mucosa by the tissue trypsinization in the isotonic Krebs solution (pH 7.9). The maximum yield of certain enterocytes is reached at the enzyme concentration of 0.03%; the incubation time--15 min; 200-250 mg of mucosa produces 3,7 X 10(6)--4,7 X 10(6) cells.
D000818:Animals; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D004386:Duodenum / Q000166:cytology*; D007413:Intestinal Mucosa / Q000166:cytology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats
K metodike polucheniia intaktnykh énterotsitov slizistoĭ dvenadtsatiperstnoĭ kishki krys.