In vitro metabolism of estradiol-17 beta by liver microsomes from juvenile rainbow trout, Salmo gairdneri.
The metabolism of [4-14C]estradiol-17 beta by liver microsomes from, Salmo gairdneri, was studied in vitro. The following metabolites were identified by gas chromatography--mass spectrometry: estrone, estra-1,3,5(10)-triene-3,16 beta,17 beta-triol, and estra-1,3,5(10)-triene-3,7 alpha,17 beta-triol. The presence of additional polar metabolites hydroxylated at positions 6 alpha and 6 beta was indicated. The formation of the hydroxylated metabolites was inhibited by CO indicating the participation of cytochrome P-450-dependent enzymes.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism*; D004964:Estriol / Q000031:analogs & derivatives / Q000378:metabolism; D004970:Estrone / Q000378:metabolism; D005260:Female; D006900:Hydroxylation; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D012487:Salmonidae / Q000378:metabolism*; D013237:Stereoisomerism; D013250:Steroid Hydroxylases / Q000378:metabolism; D014337:Trout / Q000378:metabolism*
D004970:Estrone; C037863:estra-1,3,5(10)-triene-3,7,17-triol; D004958:Estradiol; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D004964:Estriol