51Cr release cytotoxicity radioimmunoassay to detect immune cytotoxic reactions to rat Schwann cells in vitro.
Monolayers of rat Schwann cells incubated with 51Cr were used as targets for a cytotoxicity assay employing rabbit antiserum to galactocerebroside (R anti-GalC). Specific 51Cr release was demonstrated which was heat sensitive and complement-dependent. The assay was readily performed, reproducible and quantitative, and should prove useful in assessing anti-Schwann cell cytotoxic activity of experimental and human serum.
D000818:Animals; D002478:Cells, Cultured; D002857:Chromium; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D066298:In Vitro Techniques; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains; D012583:Schwann Cells / Q000276:immunology*
D002857:Chromium