A single step immunoelectrophoresis method for the quantitation of complement C3c in biological fluids.
An intermediate gel rocket immunoelectrophoresis (IRI) suitable for direct quantitation of complement split products with C3c specificity is described. The technique represents a simple, quick, and reproducible method for the assessment of complement activity in biological fluids in diseases. The advantages of the quantitative approach of the IRI method as compared to the conventional crossed immunoelectrophoresis (CIE) are the increased sensitivity, specificity, and precision of the method; also, the assay capacity is superior to CIE. The IRI-C3c assay is therefore highly suitable as a scientific tool, and may also be useful for routine laboratory investigations.
D003167:Complement Activation; D003176:Complement C3 / Q000032:analysis*; D015932:Complement C3c; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D007123:Immunoelectrophoresis, Two-Dimensional / Q000379:methods
D003176:Complement C3; D015932:Complement C3c