Plasmids in Frankia sp.
A method to achieve cell lysis and isolate Frankia sp. plasmid DNA was developed. A screening of Frankia sp. strains belonging to different host compatibility groups (Alnus sp., Elaeagnus sp., Ceanothus sp.) showed that, of 39 strains tested, 4 (strains Cp11, ARgN22d, ArI3, and EUN1f) possessed plasmids ranging in size from 7.1 to 32.2 kilobase pairs as estimated from agarose gel electrophoresis and electron microscopy. A total of 11 plasmids were detected.
D000192:Actinomycetales / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D008854:Microscopy, Electron; D010957:Plasmids; D013045:Species Specificity
D004271:DNA, Fungal