Comparative metabolism of free and esterified fatty acids by the perfused rat heart and rat cardiac myocytes.
Studies have been conducted on the uptake and metabolism of unesterified oleic acid and lipoprotein triacylglycerol by the perfused rat heart, and of oleic acid, free glycerol and lipoprotein triacylglycerol by rat cardiac myocytes. The perfused heart efficiently extracted and metabolized unesterified fatty acid and the fatty acid released during lipolysis of the recirculating triacylglycerol. The released glyceride glycerol, however, was largely accumulated in the perfusion media. Cardiac myocytes also extracted and rapidly metabolized unesterified fatty acid. As with the intact heart, free glycerol was poorly utilized by cardiac myocytes. Although the cells appeared to extract a small amount of available extracellular triacylglycerol presented as very low density lipoprotein, this was shown to be unmetabolized, suggesting adsorption rather than surface lipolysis and uptake of the released fatty acid. The data suggest that myocytes are unable to metabolize triacylglycerol fatty acids without prior lipolysis by extracellular (capillary endothelial) lipoprotein lipase.
D000818:Animals; D002250:Carbon Radioisotopes; D006352:Heart Ventricles / Q000378:metabolism; D066298:In Vitro Techniques; D008066:Lipolysis; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000378:metabolism*
D002250:Carbon Radioisotopes; D008079:Lipoproteins, VLDL; D009829:Oleic Acids; D014280:Triglycerides; D019301:Oleic Acid