Regulation of porcine heart and skeletal muscle AMP-deaminase by adenylate energy charge.
1. Cytosol from pig skeletal muscle, but not heart, contains an inhibitor of AMP-deaminase (AMP-D, EC 3.5.4.6) which reduces AMP-D activity 8-fold. 2. Heart and skeletal muscle AMP-D have been purified to apparent homogeneity by cellulose phosphate and DEAE-Sephacel chromatography. 3. AMP-D from skeletal muscle is inhibited more severely than the heart enzyme by an increase in adenylate energy charge to levels exceeding 0.4. Nevertheless both enzymes seem to be regulated by the energy charge, which contrasts with reports for rabbit heart AMP-D.
D000659:AMP Deaminase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D000227:Adenine Nucleotides / Q000494:pharmacology; D000818:Animals; D003600:Cytosol / Q000201:enzymology; D004355:Drug Stability; D004734:Energy Metabolism; D009132:Muscles / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D009709:Nucleotide Deaminases / Q000378:metabolism*; D013552:Swine
D000227:Adenine Nucleotides; D009709:Nucleotide Deaminases; D000659:AMP Deaminase