Effect of D-glucosamine on the content and synthesis of UDP-sugars and plasma membrane associated carbohydrates in chicken liver and hepatoma Mc-29.
1. Comparative studies on the effect of D-glucosamine on the synthesis of UDP-sugars and on the incorporation rate of [14C]glucose into neutral sugars, sialic acid and glucosamine isolated from chicken liver and hepatoma Mc-29 plasma membranes have been carried out. 2. The influence of D-glucosamine on the activity of microsomal UDP-N-acetylglucosamine: glycoprotein N-acetylglucosaminyltransferase to lipid (dolichol monophosphate) and to protein acceptor was studied too. 3. It has been shown that D-glucosamine provokes alterations in the content and synthesis of UDP-sugars and it is an inhibitor of the glycosylation processes. An inhibitory effect of this sugar analog on the N-acetylglycosaminyltransferase has been established.
D000818:Animals; D050260:Carbohydrate Metabolism; D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000378:metabolism; D005944:Glucosamine / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D012794:Sialic Acids / Q000096:biosynthesis; D014539:Uridine Diphosphate Sugars / Q000378:metabolism*
D002250:Carbon Radioisotopes; D012794:Sialic Acids; D014539:Uridine Diphosphate Sugars; D005947:Glucose; D005944:Glucosamine