Organ culture studies of human prostatic adenocarcinomas.
The proliferative responses of human prostatic carcinoma have been evaluated in organ culture using [125I]-iododeoxyuridine ([125I]UdR) to monitor DNA synthesis. The morphological preservation was not influenced by the addition of fetal calf serum or insulin (5 mu gm/ml), transferrin (10 mu gm/ml), and thyrotropin releasing hormone (10(-9) M) to the active medium. Testosterone (4 X 10(-7) M) stimulated [125I]UdR uptake, whereas diethylstilboestrol (4 X 10(-6) M) had no direct effect on uptake. Both estramustine phosphate (4 X 10(-6) M) and oestradiol-17 beta (4 X 10(-6) M) inhibited uptake in a similar manner. Thus while explants of human prostatic carcinoma derived from transurethrally resected specimens can be well maintained in organ culture for a few days, proliferative responses are small and difficult to measure for individual patients.
D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D003470:Culture Media; D004054:Diethylstilbestrol / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D004961:Estramustine / Q000494:pharmacology; D006801:Humans; D008297:Male; D009924:Organ Culture Techniques; D011467:Prostate / Q000187:drug effects; D011471:Prostatic Neoplasms / Q000473:pathology*; D051381:Rats; D013739:Testosterone / Q000494:pharmacology
D003470:Culture Media; D004961:Estramustine; D013739:Testosterone; D004958:Estradiol; D004054:Diethylstilbestrol