Affinity spectra: a novel way for the evaluation of equilibrium binding experiments.
For equilibrium binding isotherms of radioreceptor assays, the affinity spectrum is defined as a plot of the number of binding sites against their corresponding dissociation constants. A numerical procedure for direct calculation of affinity spectra from untransformed binding data is presented and illustrated with experimental values. The advantage of the new method in comparison to non-linear regression analysis is the fact that no starting values and mathematical models have to be supplied and that statistical assessment of the results is straightforward from a detailed graphical display of a likelihood function. Affinity spectra thus show directly all information formerly obtained by means of both graphical plots and regression analysis.
D000818:Animals; D001665:Binding Sites; D003201:Computers; D006168:Guinea Pigs; D006801:Humans; D008433:Mathematics; D008962:Models, Theoretical; D011869:Radioligand Assay / Q000379:methods*; D012044:Regression Analysis; D013057:Spectrum Analysis / Q000379:methods*