Isolation and structure of T-kinin.
T-kinin, a previously undescribed peptide containing bradykinin, has been isolated following treatment of rat plasma with trypsin (1 mg/ml). The liberated T-kinin, which contracts the rat uterus, was isolated by procedures including OM-cellulose, Biogel P-4 and reverse-phase high-performance liquid chromatography. The final material had a single N-terminal isoleucine and was shown by amino acid analysis and sequence determination to have the structure of the undecapeptide Ile-Ser-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg (isoleucyl-seryl-bradykinin). The relationships of the protein from which T-kinin is cleaved (T-kininogen) to other known kininogens is discussed.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000302:isolation & purification; D000818:Animals; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D055598:Chemical Phenomena; D002621:Chemistry; D005260:Female; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014357:Trypsin; D014590:Uterine Contraction / Q000187:drug effects
D000596:Amino Acids; C039065:T-kinin; D014357:Trypsin; D001920:Bradykinin