[Valve of the use of benzethonium chloride for turbidimetric analysis of cerebrospinal fluid proteins with an automatic analyzer].
We have adapted Iwata and Nishikaze's benzethonium chloride turbidimetric method for the assay of CSF proteins to the techniques of continuous flow and the centrifuge analyser. The proposed technique presents several advantages: benzethonium chloride forms a stable precipitate with the proteins with a slow kinetic of formation, enabling adaptation to the centrifuge analyser; the response obtained is identical for albumin and immunoglobulin G; this very sensitive technique allows the use of micro-samples. Studies of repeatability and the correlations made with other techniques give very satisfactory results.
D001322:Autoanalysis / Q000379:methods; D001558:Benzethonium; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D006801:Humans; D009391:Nephelometry and Turbidimetry / Q000379:methods; D000644:Quaternary Ammonium Compounds
D002556:Cerebrospinal Fluid Proteins; D000644:Quaternary Ammonium Compounds; D001558:Benzethonium
Intérêt de l'utilisation du chlorure de benzéthonium pour le dosage turbidimétrique des protéines du liquide céphalorachidien en analyse automatique.