Low dose electron microscopy of the crotoxin complex thin crystal.
The crotoxin complex from Crotalus d. terrificus rattlesnake venom was crystallized in the form of thin platelets. These crystals were prepared by the glucose embedding technique and examined by low dose electron microscopy. Electron diffraction patterns and images have been recorded to 2.2 and 4.5 A, respectively. By a combination of electron and X-ray diffraction techniques, the space group of this crystal was determined to be P4(2)22 with eight crotoxin complex molecules in one unit cell with dimensions of 38.8 A x 38.8 A x 256.8 A. The Patterson maps and the symmetry reliability factors calculated from the electron diffraction intensities clearly showed the existence of three types of electron diffraction patterns in different crystals. The phases in the computer-calculated transform of the low dose images also show the variation in symmetry among crystals. These phenomena are explained by the presence of crystals consisting of one-half, three-quarter and one unit cell in thickness. The interpretation of the computer reconstructed two-dimensional density map was limited, partly because of the similarity in density between the protein and the embedding glucose and partly because of the non-uniqueness in relating projected structure to the three-dimensional structure.
D000818:Animals; D003201:Computers; D003435:Crotalid Venoms / Q000302:isolation & purification; D003439:Crotoxin / Q000302:isolation & purification; D003460:Crystallization; D003461:Crystallography; D008854:Microscopy, Electron; D012911:Snakes; D014961:X-Ray Diffraction
D003435:Crotalid Venoms; D003439:Crotoxin