High-performance liquid chromatographic method for the quantitative analysis of a synthetic copolymer with antitumor activity (copovithane) and methylamine in human blood plasma and urine.
A method for the determination of a synthetic polymeric compound with antitumor activity (copovithane) and methylamine in blood plasma and urine is described. Copovithane is prepared by radical polymerisation of a diurethane with N-vinylpyrrolidone. The method is based on high-performance liquid chromatography of the methylamine hydrochloride which arises during the hydrochloric acid hydrolysis of the parent substance. The methylamine hydrochloride is converted to the trinitrobenzenesulphonyl derivative for the purpose of chromatographic detection. The limit of determination for copovithane in blood plasma is 1.2 mg/l and in urine 1.5 mg/day. The determination limit for methylamine in blood plasma is 0.2 mg/l and in urine 0.3 mg/day. The imprecision is dependent on the sample, and amounts to +/- 6.8% for blood plasma and +/- 6.4% for urine.
D000970:Antineoplastic Agents / Q000302:isolation & purification*; D002219:Carbamates / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008744:Methylamines / Q000032:analysis*; D011145:Polyvinyls / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D011205:Povidone; D014302:Trinitrobenzenesulfonic Acid
D000970:Antineoplastic Agents; D002219:Carbamates; D008744:Methylamines; D011145:Polyvinyls; C038184:copovithane; D014302:Trinitrobenzenesulfonic Acid; D011205:Povidone