Hyperimmune human ABO blood-typing sera: reactivity with murine laminin and cytotoxicity for metastatic murine tumour cells.
Commercially prepared ABO blood-typing antisera have been tested for their ability to bind to murine laminin and their cytotoxic effects upon high and low metastatic variants of a murine fibrosarcoma. Previous studies have shown that alpha-D-galactopyranosyl end-groups comprise the major antigenic determinants on type B erythrocytes and that these same end-groups are present on murine laminin purified from the EHS sarcoma and on a laminin-like glycoprotein on the surface of the high, but not low, metastatic fibrosarcoma cells. In the present study we found that all sera containing anti-B activity were cytotoxic to the high, but not the low, metastatic cells and that all of these sera reacted strongly against immobilized murine laminin in an enzyme-linked immunosorbent assay. Sera lacking anti-B activity, i.e. anti-A antisera, were much less cytotoxic to either cell line and three of the four anti-A sera did not bind to murine laminin. The laminin reactivity and cytotoxic effect of the anti-B sera were specifically abrogated by preincubation of the sera with water-soluble blood group B substance or with murine laminin but not with water-soluble blood group A substance.
D000017:ABO Blood-Group System / Q000276:immunology*; D000818:Animals; D000941:Antigens / Q000276:immunology; D002460:Cell Line; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D005354:Fibrosarcoma / Q000276:immunology* / Q000556:secondary; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D007797:Laminin; D051379:Mice; D012513:Sarcoma, Experimental / Q000276:immunology
D000017:ABO Blood-Group System; D000941:Antigens; D006023:Glycoproteins; D007106:Immune Sera; D007797:Laminin