High performance liquid chromatographic determination of caffeine in decaffeinated coffee, tea, and beverage products.
A method was developed for determining caffeine in decaffeinated coffee, tea, and beverage products by high performance liquid chromatography (HPLC). The HPLC system consisted of a Bio-Sil ODS-5S C18 column, methanol-water (25 + 75) mobile phase at 1 mL/min, and a UV detector. The method is simple and specific. Caffeine recoveries were 93.8-98.3% and coefficients of variation were 0.90-2.25%.
D001628:Beverages / Q000032:analysis*; D002110:Caffeine / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003069:Coffee / Q000032:analysis*; D013662:Tea / Q000032:analysis*
D003069:Coffee; D013662:Tea; D002110:Caffeine