Isolation of queuine from bovine amniotic fluid.
A facile method for isolation of large quantities of queuine from bovine amniotic fluid is described. Queuine was sequentially purified by cation-exchange chromatography, adsorption chromatography on Sephadex G-25, and size-exclusion chromatography on Sephadex G-10. The queuine isolate was identified by its participation in the queuine-guanine tRNA transglycosylase reaction and comparisons with authentic queuine.
D000653:Amniotic Fluid / Q000032:analysis*; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D011247:Pregnancy; D012343:RNA, Transfer / Q000302:isolation & purification
D006147:Guanine; C019549:queuine; D012343:RNA, Transfer