Automated high-performance liquid chromatographic column switching technique for the on-line clean-up and analysis of drugs in topical cream formulations.
The automation of a high-performance liquid chromatographic column switching technique for the on-line clean-up and analysis of drug substances in topical cream formulations is described. A short reversed-phase precolumn used for primary sample clean-up was coupled to a reversed-phase analytical column. This allowed cream samples dissolved in methanol-tetrahydrofuran to be injected directly into the analysis system without prior laborious and time-consuming clean-up procedures. This application was successfully extended to the analysis of sulconazole nitrate, and a triple corticoid cream. The effects of precolumns on some chromatographic parameters were studied.
D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D007093:Imidazoles / Q000032:analysis; D009824:Ointments / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000032:analysis*
D004338:Drug Combinations; D007093:Imidazoles; D009824:Ointments; D004364:Pharmaceutical Preparations; C033308:sulconazole