Surface quantification of injected fluorescein as a predictor of flap viability.
In a laboratory model, quantitative skin-surface fluorescence has been used to reliably measure skin perfusion in ischemic random flaps and to predict viability. The method is reproducible and allows investigators to sequentially monitor soft-tissue perfusion using a fluorescent indicator. It is superior to the conventional fluorescein test (Wood's lamp method), which allows only a single subjective assessment within a 24-hour period.
D000818:Animals; D005452:Fluoresceins / Q000032:analysis*; D005453:Fluorescence / Q000295:instrumentation; D006085:Graft Survival; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow; D012867:Skin / Q000098:blood supply*; D013524:Surgical Flaps; D014018:Tissue Distribution
D005452:Fluoresceins