Monoclonal antibodies to choline acetyltransferase of rat brain.
Monoclonal antibodies to rat brain choline acetyltransferase were produced by the hybridoma technique. Two stable cell lines, Ab-57 and Ab-60, secreted immunoglobulin of subclass IgG1. The monoclonal antibodies bound to choline acetyltransferase without blocking catalytic activity. Affinity of Ab-57 was 100-times higher than that of Ab-60. Both antibodies bound to the rat enzyme in a mutually exclusive fashion. The antibodies showed cross-species reactivity with choline acetyltransferase from several mammalian brains.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001921:Brain / Q000201:enzymology*; D002460:Cell Line; D002795:Choline O-Acetyltransferase / Q000276:immunology*; D003429:Cross Reactions; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats
D000911:Antibodies, Monoclonal; D002795:Choline O-Acetyltransferase