Reduced and oxidized ascorbates in guinea pig retina under normal and light-exposed conditions.
Both reduced and oxidized ascorbates were measured in aqueous, neural retina, and pigment epithelium-choroid complex (PE-C) of pigmented guinea pigs. Normal values for total ascorbate of 16 mg/dl in aqueous, 22 mg/dl in neural retina, and 7 mg/dl in PE-C were found. After mild photic damage caused by varying lengths of exposure of 10,000 to 20,000 lux of fluorescent lighting, reduced ascorbate concentrations generally decreased in the neural retina, while oxidized ascorbate generally increased in PE-C. In both normal and light-exposed retinas, reduced ascorbate was predominant in the neural retina, and oxidized ascorbate was predominant in the PE-C. Histochemical localization of reduced ascorbate occurred in the Müller cell fibers and at the apices of the retinal pigment epithelium.
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D002829:Choroid / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003683:Dehydroascorbic Acid / Q000378:metabolism; D006168:Guinea Pigs; D006651:Histocytochemistry; D010084:Oxidation-Reduction; D010857:Pigment Epithelium of Eye / Q000378:metabolism; D012160:Retina / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D012614:Scurvy / Q000378:metabolism
D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid