Autoradiographic localization of androgen binding in the developing mouse prostate.
The ontogeny of expression of [3H]dihydrotestosterone (3H-DHT) binding in the developing mouse prostate was studied using steroid autoradiography. At all prenatal stages examined, 3H-DHT binding in the urogenital sinus was restricted to the stromal tissue compartment. This pattern of binding continued until approximately day 4 of postnatal life, when some epithelial cells began to exhibit nuclear localization of 3H-DHT. The pattern of binding was asynchronous within the prostate, with the onset of nuclear labeling seemingly correlated with canalization of prostatic ducts. By 3 weeks of age, virtually all prostatic epithelial cells exhibited nuclear labeling with 3H-DHT. The significance of these results, with respect to the role of epithelial-mesenchymal interactions in hormone-induced development, are discussed.
D000367:Age Factors; D000728:Androgens / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011467:Prostate / Q000196:embryology* / Q000254:growth & development
D000728:Androgens; D013196:Dihydrotestosterone