Chemical characterization of extracellular polysaccharides produced by Actinomyces viscosus T14V and T14Av.
The human isolates Actinomyces viscosus T14V and T14Av produced extracellular polysaccharides in the absence of sucrose. In contrast to strain T14V, strain T14Av produced abundant viscous slime polysaccharide in the culture supernatant fluids when grown in a chemically defined medium containing glucose. After resolution of the T14Av polysaccharides into seven fractions, it was demonstrated that two of these exhibited viscous properties and lacked methylpentose and muramic acid. The major slime polysaccharide purified by gel filtration and ion-exchange column chromatography contained 39% (moles percent carbohydrate) galactose, 37% N-acetylglucosamine, 19% glucose, and 5% mannose. Only trace amounts of protein and phosphorus were detected in this preparation. On the other hand, strain T14V produced negligible slime polysaccharide under the same culture conditions. The major extracellular polysaccharide fraction from this strain contained methylpentoses, hexoses, hexosamines, muramic acid, protein, and phosphorus, suggesting that this fraction might be derived from the cell wall.
D000190:Actinomyces / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D005690:Galactose / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D005947:Glucose / Q000032:analysis; D008358:Mannose / Q000032:analysis; D010758:Phosphorus / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000302:isolation & purification
D002241:Carbohydrates; D011135:Polysaccharides, Bacterial; D010758:Phosphorus; D005947:Glucose; D005944:Glucosamine; D008358:Mannose; D005690:Galactose