The recognition of a soluble cytochrome P450 in rat liver.
The presence of hepatic soluble cytochrome P450 was investigated in the rats that were pretreated with CCl4, CS2 and DENA + PCBs. The dithionite-reduced CO difference spectrum of the hepatic 204000 x g supernatant of these rats showed its maximum at 455 nm. Aniline and hexobarbital could react with the hepatic 204000 x g supernatant to give the substrate binding spectra of P450; differences were observed between the substrate-induced difference spectra of the 204000 x g supernatant and that of the microsomal fraction. These results indicate the existence of a hepatic soluble P450.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D012995:Solubility; D013053:Spectrophotometry
D003577:Cytochrome P-450 Enzyme System