In-vitro fertilization of rabbit ova ovulated in vitro during ovarian perfusion.
During in-vitro perfusion of rabbit ovaries, ova that ovulated in response to hCG were recovered and cultured with spermatozoa capacitated in vivo. After culture for 30 h, 113 (48.8%) of the ova underwent cleavage and were not degenerate. Of these 113 zygotes, 75 were successfully transferred to the oviducts of 14 gonadotrophin-treated host rabbits. On Day 14 after transfer there were 14 implantation sites and 9 pregnancies proceeded to term. These data indicate that in-vitro ovulated ova can be fertilized in vitro and be used to establish normal pregnancies in a host rabbit.
D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D005260:Female; D005307:Fertilization in Vitro; D066298:In Vitro Techniques; D010053:Ovary / Q000187:drug effects / Q000502:physiology; D010060:Ovulation / Q000187:drug effects*; D010477:Perfusion; D011247:Pregnancy; D011817:Rabbits
D006063:Chorionic Gonadotropin