The DNA extruded by rat spleen cells in culture.
1. Part of the DNA extruded by cultured rat spleen cells occurs in the form of a low density lipid complex. 2. The present work indicates that this complex is also associated with protein but not with RNA and its elementary properties have been studied. 3. For example, it is destroyed by freezing and thawing or by deoxycholate treatment and small quantities of copper salts added to the medium may either inhibit or stimulate its release depending on the conditions.
D000818:Animals; D002478:Cells, Cultured; D003300:Copper / Q000494:pharmacology; D004247:DNA / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects; D003840:Deoxycholic Acid / Q000494:pharmacology; D003852:Deoxyribonucleoproteins / Q000378:metabolism; D005260:Female; D007700:Kinetics; D051381:Rats; D013154:Spleen / Q000187:drug effects / Q000378:metabolism*
D003852:Deoxyribonucleoproteins; D003840:Deoxycholic Acid; D003300:Copper; D004247:DNA