title: Soluble fractions of Pasteurella multocida: their protective qualities against fowl cholera in turkeys.
abstract: Soluble fractions of Pasteurella multocida strain P1059 were extracted from a single source by four methods, and their immunogenicity was evaluated by challenge exposure in turkeys. The fractions were extracted by 1) heating in 2.5% NaCl, 2) 0.5M potassium thiocyanate, 3) 1.0M sodium salicylate, and 4) prolonged stirring in formalin solution followed by pelleting (LPS-protein antigen). Eighty percent to 90% of infected turkeys were protected in two trials by vaccination with the saline extract or LPS-protein antigen, whereas less consistent protection was associated with the other two preparations. Endotoxin content was the highest in LPS-protein antigen, followed by KSCN, Na salicylate, and saline extract in that order. The four fractions contained at least one common antigen, which had previously been shown to be a surface-protective antigen.
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology; D004731:Endotoxins / Q000032:analysis; D008070:Lipopolysaccharides / Q000276:immunology; D010325:Pasteurella / Q000276:immunology; D010326:Pasteurella Infections / Q000517:prevention & control / Q000662:veterinary*; D011201:Poultry Diseases / Q000517:prevention & control*; D012995:Solubility; D013347:Subcellular Fractions / Q000032:analysis; D014422:Turkeys
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D004731:Endotoxins; D008070:Lipopolysaccharides