Relative effectiveness of 241Am vs 226Ra approached by haemopoietic stem cell studies in various bone marrow sites of contaminated mice.
Haemopoietic stem cell assays allow investigation of local radiation damage at any time after contamination. These techniques were used to determine the relative effectiveness of 241Am and 226Ra in inducing damage in various cortical and trabecular bone marrow sites. Male Balb/c mice were injected with either 230 or 660 kBq 226RaCl2/kg body weight or with one of three doses of monomeric 241Am citrate at 138, 552 or 768 kBq 241Am/kg body weight. At 7 time intervals after injection (from 4 hr to 100 days) the colony-forming capacity of pluripotent stem cells (= CFU-s) and macrophage-granulocyte committed stem cells (CFU-c) was assayed in sternal marrow, marrow of lumbar vertebrae, of the trabecular distal end of the femur and of the femoral shaft (in which were distinguished axial marrow and marrow situated peripherally near the cortical bone). 226Ra retention and 241Am retention were measured in these bone sites. Skeletal doses and doses to the bone marrow sites were calculated. On the base of injected activity, 241Am was more effective than 226Ra in reducing the number of CFU-s and CFU-c but the effectiveness varied in the various bone marrow sites. CFU-s and CFU-c response is considered in relation to the retained radioactivity in the surrounding bone matrix and in relation to the mean marrow dose.
D000576:Americium / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects; D001842:Bone and Bones / Q000378:metabolism; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000528:radiation effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D011833:Radiation Injuries, Experimental / Q000378:metabolism / Q000473:pathology; D011883:Radium / Q000378:metabolism / Q000494:pharmacology*; D012062:Relative Biological Effectiveness; D013997:Time Factors
D000576:Americium; D011883:Radium