Effects of hexafluoroacetone on testicular morphology and lipid metabolism in the rat.
Rats dosed dermally with 39 or 130 mg/kg/day hexafluoroacetone sesquihydrate (HFA) for 14 days developed moderate or severe testicular atrophy, respectively; rats dosed with 13 mg/kg/day HFA for 14 days did not. Histologic evaluation of the testes revealed that spermatids, followed by spermatocytes, were the germ cells most affected by HFA; spermatogonia and Sertoli cells appeared to be less vulnerable. Lipogenesis from [3H]acetate and[14C]glucose was investigated in vitro in testes from HFA-treated and pair-fed control rats. Triacylglycerol and phospholipid synthesis was increased whereas sterol synthesis was decreased in testes from HFA-treated rats. Vitamin A and zinc were measured in the testes of control and HFA-treated rats; no differences in the levels of these nutrients were observed between the two groups. The data support the hypothesis that altered lipid metabolism, in particular sterol metabolism, is associated with the development of HFA-induced testicular atrophy.
D000096:Acetone / Q000031:analogs & derivatives* / Q000633:toxicity; D000818:Animals; D001284:Atrophy / Q000139:chemically induced; D005466:Fluorocarbons / Q000633:toxicity*; D005947:Glucose / Q000378:metabolism; D050356:Lipid Metabolism; D008297:Male; D051381:Rats; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006845:Hydrocarbons, Fluorinated
D000096:Acetone; D005466:Fluorocarbons; D005947:Glucose; C017366:hexafluoroacetone; D006845:Hydrocarbons, Fluorinated