title: Stimulation of human monocyte/macrophage-derived growth factor (MDGF) production by plasma fibronectin.
abstract: Culture supernatants from human peripheral blood monocytes, isolated free of platelet contamination and cultured in the absence of serum, stimulate DNA synthesis and cell growth in Balb/c 3T3 fibroblasts and bovine aortic smooth-muscle cells. Monocytes cultured in serum-free medium for 24 hours with plasma fibronectin, added as either a surface-attached or soluble molecule, secrete significantly increased amounts of growth-promoting activity. Fibronectin also stimulates an increase in intracellular growth factor content and in protein synthesis by monocytes. Both the enhanced growth-promoting activity and protein synthesis are inhibited by cycloheximide. Thus, fibronectin-monocyte interactions may influence the production of growth-promoting activity by monocytes and contribute to fibroblast and smooth-muscle replication in wound healing, chronic inflammation and atherosclerosis.
mesh_terms: D002448:Cell Adhesion; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005353:Fibronectins / Q000494:pharmacology*; D006133:Growth Substances / Q000378:metabolism*; D006801:Humans; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D013268:Stimulation, Chemical
chemical_list: D005353:Fibronectins; D006133:Growth Substances; D004247:DNA