Temperature-sensitive conformational changes in membrane-bound and solubilized [3H]imipramine binding sites.
[3H]Imipramine binding sites are located on serotonergic nerve terminals and on blood platelets. Previous studies on our laboratories have indicated temperature-sensitive conformational changes in [3H]imipramine binding sites in hippocampal and cerebral cortex membranes. We now report that similar changes are observed in both membrane-bound and digitonin-solubilized [3H]imipramine binding sites of human blood platelets. This may indicate integrity of the solubilized binding site.
D000818:Animals; D001665:Binding Sites; D001792:Blood Platelets / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D006801:Humans; D007099:Imipramine / Q000378:metabolism*; D066298:In Vitro Techniques; D011487:Protein Conformation; D051381:Rats; D012995:Solubility; D013696:Temperature; D014316:Tritium
D014316:Tritium; D007099:Imipramine