Enzyme-linked immunosorbent assay for use with nuclear protein-DNA complex antigens.
An enzyme-linked immunosorbent assay (ELISA) was developed for identification and quantification of nuclear antigens (nonhistone protein-DNA complexes from chromatin). Until now, the complement fixation assay has been the only immunoassay routinely applied to nonhistone protein-DNA complexes. The ELISA is considerably more sensitive than the micro-complement fixation test for assaying the immunospecificity of nuclear protein-DNA complexes. Dilutions of rabbit antisera as great as 1:6400 could be used to detect nanogram quantities of antigen, chicken reticulocyte chromatin or dehistonized rat liver chromatin.
D000818:Animals; D000917:Antibody Formation; D034961:Antigens, Nuclear; D002645:Chickens / Q000276:immunology; D002843:Chromatin / Q000276:immunology; D003168:Complement Fixation Tests; D004247:DNA / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D066298:In Vitro Techniques; D009698:Nucleoproteins / Q000276:immunology*; D011817:Rabbits; D012156:Reticulocytes / Q000276:immunology
D034961:Antigens, Nuclear; D002843:Chromatin; D009698:Nucleoproteins; D004247:DNA