Binding of optically pure (--)-[3H]nicotine to rat brain membranes.
With the recent availability of (--)-[3H]nicotine of high specific activity, binding studies were performed on rat brain membranes in the presence of a variety of nicotine analogues and cholinergic drugs. Both a higher affinity (Kd = 2 X 10(-10) M) and a lower affinity (2 X 10(-9) M) site were observed; the stereoselectivity of both sites being similar. A good correlation was observed between IC50-values and psychotropic potency of a series of N'-alkyl substituted nicotine analogues.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D066298:In Vitro Techniques; D009538:Nicotine / Q000378:metabolism*; D051381:Rats; D011978:Receptors, Nicotinic / Q000378:metabolism; D013237:Stereoisomerism
D011978:Receptors, Nicotinic; D009538:Nicotine