Boehringer immunoinhibition procedure for creatine kinase-MB evaluated and compared with column ion-exchange chromatography.
In determination of creatine kinase isoenzyme MB (CK-MB), the Boehringer immunoinhibition method gives a high and variable blank activity as compared with column-chromatography. Thus a correction must be applied. Furthermore, a second correction of 1% of total creatine kinase activity is necessary to compensate for nonspecific creatine phosphate-dependent activity. As a consequence, two immunoinhibition determinations--one for CK-MB and one for blank activity--and a determination of total creatine kinase are required. Use of the manufacturer's diagnostic criteria, on the basis of which suspected myocardial infarction is confirmed or eliminated, leads to a high frequency of false-negative conclusions.
D002852:Chromatography, Ion Exchange; D003402:Creatine Kinase / Q000097:blood*; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D006801:Humans; D007527:Isoenzymes; D009203:Myocardial Infarction / Q000175:diagnosis; D011933:Reagent Kits, Diagnostic
D007527:Isoenzymes; D011933:Reagent Kits, Diagnostic; D003402:Creatine Kinase