Distributions of 35S-sulfate and 3H-glucosamine in the angular region of the hamster: light and electron microscopic autoradiography.
The distribution of 35S-sulfate and 3H-glucosamine in the angular region of the hamster was studied by light and electron microscopic autoradiography following intraperitoneal injection of these compounds to hamsters. Exposed silver grains of 35S-sulfate were concentrated in the trabecular meshwork, sclera, and cornea, and grains of 3H-glucosamine were localized in the trabecular region. The radioactivity of both isotopes was observed in the Golgi apparatuses of the endothelial cells of the angular aqueous plexus and the trabecular meshwork. The grains were noted over the entire cytoplasm, except for the nucleus, and then were incorporated into the amorphous substance and collagen fibers in the region adjacent to the angular aqueous sinus. These results suggest that endothelial cells in the angular region synthesize and secrete the sulfated glycosaminoglycans and hyaluronic acid.
D000818:Animals; D000867:Anterior Chamber / Q000032:analysis*; D001082:Aqueous Humor / Q000032:analysis; D001345:Autoradiography; D006224:Cricetinae; D005944:Glucosamine / Q000032:analysis*; D006025:Glycosaminoglycans / Q000032:analysis*; D008854:Microscopy, Electron; D013431:Sulfates / Q000032:analysis*; D013462:Sulfur Radioisotopes; D014129:Trabecular Meshwork / Q000032:analysis; D014316:Tritium
D006025:Glycosaminoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes; D014316:Tritium; D005944:Glucosamine