Angiotensinogen.
Human angiotensinogen has been purified from outdated blood bank plasma. The purified angiotensinogen has a specific angiotensin I (Ang I) content of 20.7 micrograms Ang I/mg protein and contains only one amino-terminal amino acid, aspartic acid. However, it exhibits two bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with apparent molecular weights of 61,400 and 65,400. It is concluded that these bands represent different forms of angiotensinogen because the specific Ang I content is equivalent to the theoretical Ang I content. Analysis of the purified angiotensinogen showed it to contain 14% carbohydrate.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000808:Angiotensinogen / Q000097:blood / Q000302:isolation & purification*; D000809:Angiotensins / Q000302:isolation & purification*; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D006973:Hypertension / Q000097:blood; D008970:Molecular Weight; D011247:Pregnancy; D011249:Pregnancy Complications, Cardiovascular / Q000097:blood; D051381:Rats; D013045:Species Specificity; D013552:Swine
D000596:Amino Acids; D000809:Angiotensins; D000808:Angiotensinogen