[Characteristics of a low-molecular nonvirion ("soluble") antigen from the tick-borne encephalitis virus].
Pig embryo kidney and BHK-21 cells infected with tick-borne encephalitis virus synthesize a nonvirion antigen differing in its immunological properties from the virion antigens. The antigen has a high thermostability. According to the results of ultrafiltration and gel filtration, its molecular weight is approximately 70-100 kilodaltons. Electrophoretic analysis in polyacrylamide gel showed the molecular weight of a polypeptide isolated from the precipitation band formed by this low molecular antigen to differ from molecular weights of virion and high molecular virus-specific nonvirion proteins and to be about 20 kilodaltons. This polypeptide is assumed to be a subunit of the analyzed nonvirion low molecular antigen.
D000956:Antigens, Viral / Q000032:analysis* / Q000302:isolation & purification; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology*; D007122:Immunoelectrophoresis; D008970:Molecular Weight; D012995:Solubility; D014462:Ultrafiltration; D014776:Virus Cultivation
D000956:Antigens, Viral
Kharakteristiki nizkomolekuliarnogo nevirionnogo ("rastvorimogo") antigena virusa kleshchevogo éntsefalita.