Separation of tetrodotoxin and paralytic shellfish poisons by high-performance liquid chromatography with a fluorometric detection using o-phthalaldehyde.
Tetrodotoxin (TTX) and a variety of paralytic shellfish poisons (PSPs) were extracted from toxic specimens of puffer and scallop, and quantitated by high-performance liquid chromatography with a fluorometric detection using o-phthalaldehyde. Fluorescence spectra for the TTX- and PSP-fluorophors in 0.05 M borate buffer (pH 10) showed maxima at 453 nm with 332-nm excitation. The fluorescence intensity per nM of TTX was found to be 3 and those of PSP to be 4-12. This fluorometric technique may be useful for the simultaneous quantitation of TTX and PSP in small volumes of toxin extracts.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008297:Male; D008387:Marine Toxins / Q000302:isolation & purification*; D051379:Mice; D008978:Mollusk Venoms / Q000302:isolation & purification; D012758:Shellfish; D013050:Spectrometry, Fluorescence; D013779:Tetrodotoxin / Q000302:isolation & purification*; D009764:o-Phthalaldehyde
D008387:Marine Toxins; D008978:Mollusk Venoms; D013779:Tetrodotoxin; D009764:o-Phthalaldehyde