Anomalous electrophoretic migration of oligodeoxynucleotides with terminal -OH groups: applications for DNA exonuclease characterization.
Oligodeoxynucleotides with a terminal -OH group on both the 5' and 3' ends migrate anomalously in 23% polyacrylamide-7 M urea gels. This migration anomaly can be exploited to characterize nuclease digestion products. Thus, using specific substrates and the methods described, several types of DNA exonuclease activity can be readily distinguished.
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005090:Exodeoxyribonucleases / Q000032:analysis*; D006801:Humans; D006900:Hydroxylation; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000032:analysis*; D009841:Oligonucleotides / Q000032:analysis*; D013379:Substrate Specificity
D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D005090:Exodeoxyribonucleases