Agglutination of murine and guinea pig peritoneal cells by alpha-L-fucose-binding lectin: Evonymus europaea.
Among lectins from Lotus tetragonolobus, Ulex europaeus I and Evonymus europaea, agglutinating cells with blood group H determinants containing L-fucose alpha 1 leads to 2-linked to subterminal D-galactose, only the last lectin agglutinates thioglycolate- and paraffin oil-stimulated murine and guinea pig peritoneal exudate cells (PEC). The agglutination is inhibited by specific inhibitors of Evonymus lectin only: lacto-N-fucopentaose I and lactose. These results suggest the presence of a determinant on the surface of PEC, containing L-fucose alpha 1-linked at the nonreducing end which is different from blood group H determinants. Nonstimulated murine peritoneal cells (PC) are not agglutinated by the lectin but become agglutinable after neuraminidase treatment. Unstimulated guinea pig PC from different animals are agglutinated to a different extent by the same lectin concentration and show increased agglutinability after neuraminidase digestion. These results show that receptor for Evonymus lectin also exists on the nonstimulated PC but access to it is hindered by sialic acid. Trypsin- and pronase-digested PEC show increased agglutinability with Evonymus lectin. These results suggest that the lectin receptor is a glycolipid. Since alpha-linked L-fucose has been suggested as a part of the macrophage receptor for migration inhibitory factor in the guinea pig (Remold, J. Exp. Med. 1973. 138: 1065), the effect of Evonymus europaea lectin on the migration of PEC was studied. It was found that lectin inhibits the migration of PEC in the capillary tube assay up to 80%.
D000371:Agglutination; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology* / Q000276:immunology; D001667:Binding, Competitive; D002464:Cell Migration Inhibition; D005260:Female; D006168:Guinea Pigs; D006384:Hemagglutination; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009844:Oligosaccharides / Q000494:pharmacology; D037121:Plant Lectins; D011975:Receptors, Mitogen / Q000032:analysis
D037102:Lectins; D009844:Oligosaccharides; D037121:Plant Lectins; D011975:Receptors, Mitogen; C036850:Ulex europaeus lectins; C027112:fucose-binding lectin