[Physicochemical characteristics of a vaccinal strain of the Leningrad-3 mumps virus (L-3)].
Purified virions of the vaccine strain Leningrad-3 of mumps virus propagated in Japanese quail embryo cell cultures had a buoyant density 1.18-1.19 g/ml in sucrose gradient, contained 50 S RNA and showed variable sizes in electron microscopy as manifested by heterogeneity of the virus zone in sedimentation analysis. Purified L-3 virus contained 5 major polypeptides with molecular weights of 74,000, 68,000, 58,000, 45,000, and 39,000 daltons. Each polypeptide had an individual oligopeptide composition.
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D008970:Molecular Weight; D009108:Mumps Vaccine / Q000032:analysis*; D009109:Mumps virus / Q000032:analysis* / Q000302:isolation & purification / Q000648:ultrastructure; D009842:Oligopeptides / Q000032:analysis; D010455:Peptides / Q000032:analysis; D014771:Virion / Q000032:analysis / Q000648:ultrastructure
D009108:Mumps Vaccine; D009842:Oligopeptides; D010455:Peptides
Fiziko-khimicheskaia kharakteristika vaktsinnogo shtamma virusa parotita Leningrada-3 (L-3).