Radioimmunoassay for antibodies to human skeletal muscle myosin in serum from patients with polymyositis.
Antibodies to human skeletal muscle myosin were detected in 90% of sera from patients with polymyositis by radioimmunoassay using purified human myosin as antigen, and the mean titre was 4.24 X 10(-10)M. The incidence and the mean titre of anti-myosin antibodies were significantly higher than in patients without polymyositis. Anti-myosin antibody titres correlated with steroid therapy in polymyositis patients. Titres in untreated patients with polymyositis correlated with the severity of muscle weakness. Radioimmunoassay for anti-myosin antibodies should prove to be useful in the diagnosis of polymyositis and the evaluation of clinical state.
D000328:Adult; D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009132:Muscles / Q000503:physiopathology; D009218:Myosins / Q000276:immunology*; D009220:Myositis / Q000188:drug therapy / Q000276:immunology* / Q000503:physiopathology; D011863:Radioimmunoassay; D013256:Steroids / Q000627:therapeutic use
D001323:Autoantibodies; D013256:Steroids; D009218:Myosins