title: Activated carbon beads for the removal of highly albumin-bound species.
abstract: Activated carbon beads in which 5% activated carbon powder was embedded in 4% agarose were prepared by the emulsion technique. The column of the beads was demonstrated to effectively remove salicylic acid, warfarin, and long-chain fatty acids from solutions containing albumins by either zonal or frontal analysis under the condition of 0.1 M NaCl, pH 3.0 (HCl). The beads were also demonstrated to purify a commercial human serum albumin preparation from residual fatty acids. The beads would be of value in many biochemical purification processes in which activated carbon is employed.
mesh_terms: D000327:Adsorption; D000818:Animals; D002244:Carbon; D002417:Cattle; D005227:Fatty Acids / Q000302:isolation & purification; D006801:Humans; D011485:Protein Binding; D012459:Salicylates / Q000302:isolation & purification; D012709:Serum Albumin / Q000032:analysis*; D014859:Warfarin / Q000302:isolation & purification
chemical_list: D005227:Fatty Acids; D012459:Salicylates; D012709:Serum Albumin; D014859:Warfarin; D002244:Carbon