title: Induction of secondary antibody responses to Plasmodium chabaudi in vitro.
abstract: Spleen cells and peripheral blood mononuclear cells (PBMC) from mice, which had recovered from infection with Plasmodium chabaudi, were induced to produce anti-P. chabaudi antibody by incubating the cells with P. chabaudi parasitized red cells in Marbrook cultures. The anti-malarial antibody was assayed using the indirect fluorescent antibody test. Spleen cells and PBMC from mice infected 2-4 months previously gave higher antibody titres in culture than similar cells from mice infected a year previously. There was a good correlation between the ability of spleen cells or PBMC to be stimulated to produce antibody in vitro and the ability of mice similar to the cell donors to resist a challenge infection. Some immunity can be adoptively transferred with both spleen cells and PBMC.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D001403:Babesia / Q000276:immunology; D001404:Babesiosis / Q000276:immunology; D002478:Cells, Cultured; D007116:Immunization, Passive; D007156:Immunologic Memory; D008214:Lymphocytes / Q000276:immunology; D008288:Malaria / Q000276:immunology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains; D010961:Plasmodium / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013997:Time Factors