Development of gluconeogenesis in isolated hepatocytes from the rabbit.
Gluconeogenesis from 10 mM lactate has been studied in isolated hepatocytes from fetal, newborn, and 70-day-old rabbits. Gluconeogenesis proceeds to a very low rate in fetal rabbit hepatocytes despite substantial activities of all gluconeogenic enzymes including mitochondrial phosphoenolpyruvate carboxykinase. A tenfold increase in the rate of gluconeogenesis occurs in hepatocytes from 1- or 2-day-old fasting or suckling newborn rabbits. The emergence of gluconeogenic capacity in newborn rabbit hepatocytes is triggered by birth itself and not by a chronological factor, and it is primarily controlled by an increase in the activity of cytosolic phosphoenolpyruvate carboxykinase. Moreover, an active fatty acid oxidation is essential to support a high rate of gluconeogenesis in hepatocytes from newborn rabbits.
D000375:Aging; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology; D005227:Fatty Acids / Q000378:metabolism; D005943:Gluconeogenesis; D006003:Glycogen / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008099:Liver / Q000196:embryology / Q000378:metabolism*; D010084:Oxidation-Reduction; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism; D011817:Rabbits
D005227:Fatty Acids; D007773:Lactates; D019344:Lactic Acid; D006003:Glycogen; D010729:Phosphoenolpyruvate Carboxykinase (GTP)