NKP-15: a monoclonal antibody reactive against purified human natural killer cells and granulocytes.
A monoclonal antibody of the isotype IgG1,k, NKP-15, was produced by immunizing Balb/c mice with highly purified suspensions of human natural killer (NK) cells. By indirect immunofluorescence, NKP-15 reacted with 15 +/- 5.0% of the peripheral blood lymphocytes, 90 +/- 3.0% of the granulocytes and was non-reactive with T-cells, B-cells, monocytes, platelets and erythrocytes. NKP-15 reacted with 90 +/- 6.0% of purified large granular lymphocytes (LGL), which displayed highly enriched NK cell activity. NKP-15, however, showed no reactivity with a panel of human tumor cell lines. Analysis of NK cell-K562 tumor cell conjugates and depletion by panning of NKP-15+ cells indicated that all functionally tumorlytic NK cells expressed the NKP-15 antigen. It was also determined by competitive blocking experiments that NKP-15 recognizes a new NK cell surface antigen not delineated by the NK-specific monoclonal antibody, Leu-7, and that the epitope for NKP-15 appears to be associated with the Fc-receptor of NK cells and granulocytes.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D006098:Granulocytes / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007126:Immunoglobulin Allotypes / Q000032:analysis; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011961:Receptors, Fc / Q000032:analysis
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007126:Immunoglobulin Allotypes; D011961:Receptors, Fc