title: In vitro immune mechanisms associated with clearance of microfilariae of Dirofilaria immitis.
abstract: An in vitro system has been developed to elucidate potential immune mechanisms associated with clearance of microfilariae (Mf) from the bloodstream in canine Dirofilaria immitis infection. Granulocytes as well as mononuclear cells adhere to Mf of Dirofilaria immitis in the presence of immune serum. Only granulocytes, however, were capable of killing Mf, whereas PBMC attach to but do not effectively kill Mf. In the presence of granulocytes 1% +/- 1, 10% +/- 2, and 12% +/- 3 of Mf were killed by heated normal (NDS), patent (PS), and occult serum (OS), respectively, after an 18-hr incubation. With the addition of fresh NDS there was an increase in killing to 5% +/- 1 (p less than 0.025) with heat-inactivated NDS, to 12% +/- 3 in the presence of PS and to 77% +/- 12 (p less than 0.005) in the presence of OS. On further purification of granulocyte cell populations with metrizamide gradients, neutrophils were found to be the predominant effector cells with 73% +/- 18 killing with neutrophils and 18% +/- 6 with eosinophils (p less than 0.0005). Only with neutrophils was a significant increase in killing of Mf observed when fresh NDS was added to delta OS. Fractionation of OS by gel filtration suggested that IgM was the opsonizing antibody in the occult serum. In addition, immunofluorescent studies showed only IgM bound to the surface on Mf on incubation in OS. The involvement of complement in the fresh serum enhancement of killing was supported by the finding, by immunofluorescence, of surface C3 on Mf after incubation in fresh OS.
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002470:Cell Survival; D003176:Complement C3 / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D004183:Dirofilaria immitis / Q000276:immunology / Q000648:ultrastructure; D004184:Dirofilariasis / Q000276:immunology*; D004285:Dogs; D006098:Granulocytes / Q000276:immunology; D007111:Immunity, Cellular; D009000:Monocytes / Q000276:immunology; D009895:Opsonin Proteins
chemical_list: D003176:Complement C3; D009895:Opsonin Proteins