title: Immunochemical studies of yellowjacket venom proteins.
abstract: The major proteins of yellowjacket venoms have been isolated and characterized immuno-chemically. They consist of hyaluronidase, phospholipase, and antigen 5. Venoms from three species of yellowjacket were studied. Vespula germanica, V. maculifrons, and V. vulgaris. The phospholipases could be isolated in good yield only when affinity chromatography was used to minimize limited proteolysis. A kallikrein-like peptidase was found present in the yellowjacket venom. Phospholipases from these three species were immunochemically indistinguishable from each other, as were their antigen 5s. Sera from individuals sensitive to yellowjacket venom contained IgE and IgG specific for antigen 5 and phospholipase.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000941:Antigens / Q000276:immunology / Q000302:isolation & purification; D001514:Bee Venoms / Q000276:immunology*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000276:immunology / Q000302:isolation & purification; D007073:Immunoglobulin E / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D010740:Phospholipases / Q000276:immunology / Q000302:isolation & purification; D011506:Proteins / Q000276:immunology* / Q000302:isolation & purification; D014862:Wasp Venoms / Q000276:immunology*
chemical_list: D000596:Amino Acids; D000941:Antigens; D001514:Bee Venoms; D007074:Immunoglobulin G; D011506:Proteins; D014862:Wasp Venoms; D007073:Immunoglobulin E; D010740:Phospholipases; D006821:Hyaluronoglucosaminidase