beta-Carboline binding to deoxycholate solubilized benzodiazepine receptors from calf cerebral cortex.
The beta-carbolines harmane and norharmane competitively inhibit [3H]flunitrazepam ([3H]FNZ) binding to deoxycholate-solubilized benzodiazepine receptors from calf cerebral cortex, with Ki in the micromolar range [3H]Propyl-beta-carboline-3-carboxylate ([3H]PrCC) binds to the soluble receptors with an affinity similar to its binding to particulate receptors (0.41 nM vs 0.48 nM, respectively). The component that binds [3H]PrCC displays a sedimentation profile on sucrose gradient centrifugation similar to that of [3H]FNZ binding component (sedimentation coefficient about 11S).
D000470:Alkaloids / Q000378:metabolism*; D000818:Animals; D001569:Benzodiazepines / Q000502:physiology*; D002243:Carbolines / Q000378:metabolism; D002417:Cattle; D002540:Cerebral Cortex / Q000378:metabolism*; D003840:Deoxycholic Acid / Q000494:pharmacology*; D005445:Flunitrazepam / Q000378:metabolism; D006247:Harmine / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D011955:Receptors, Drug / Q000187:drug effects / Q000378:metabolism*; D012995:Solubility; D013223:Statistics as Topic; D014316:Tritium
D000470:Alkaloids; D002243:Carbolines; D011955:Receptors, Drug; D003840:Deoxycholic Acid; D014316:Tritium; D001569:Benzodiazepines; D006247:Harmine; D005445:Flunitrazepam; C028585:propyl beta-carboline-3-carboxylate; C005010:harman; C010262:norharman