Histochemical staining. Its use for detection of glyceraldehyde-3-phosphate dehydrogenase in skeletal muscle.
Histochemical staining of glyceraldehyde-3-phosphate dehydrogenase activity in fresh-frozen muscle tissue may be performed using a beta-nicotinamide adenine dinucleotide (beta-NAD) reduced-dehydrogenase tetrazolium-reductase linked reaction. The phosphate-buffered incubating medium contains glyceraldehyde-3-phosphate, beta-NAD, nitro blue tetrazolium, and edetic acid. This technique produces a fine reticular pattern of blue staining in muscle fibers. In human muscle specimens, type I fibers stain darker than type II fibers.
D000818:Animals; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000032:analysis*; D006801:Humans; D008297:Male; D009132:Muscles / Q000201:enzymology* / Q000473:pathology; D009243:NAD; D009580:Nitroblue Tetrazolium; D051381:Rats
D009243:NAD; D009580:Nitroblue Tetrazolium; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases