Synthesis of uridine oligonucleotide by the reaction of unprotected uridine with tri-(imidazolyl-(1)) phosphine.
Uridine oligonucleotide was synthesized by the reaction of unprotected uridine with tri-(imidazolyl-(1))phosphine and the successive oxidation of the resulting cyclic phosphite with iodine and water. The uridine oligonucleotide obtained from this reaction contained both 3'-5'linkage and 2'-5'linkage. The type of the phosphodiester linkage was resulted at the oxidative ring-opening reaction, and it was controlled by polynucleotides and various divalent metal cations.
D002413:Cations, Divalent; D007202:Indicators and Reagents; D008722:Methods; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds; D014500:Uracil Nucleotides / Q000138:chemical synthesis*; D014529:Uridine
D002413:Cations, Divalent; D007202:Indicators and Reagents; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D009943:Organophosphorus Compounds; D014500:Uracil Nucleotides; C027916:oligo(U); C031085:tris(imidazol-1-yl)phosphine; D014529:Uridine