title: Cellular basis for differences in humoral immune responses of sheep immunized with living or killed Staphylococcus aureus vaccines.
abstract: An immunohistological study was made of immunoglobulin-containing cells and antibody-containing cells in abscesses/granulomas which formed at the injection site, draining popliteal nodes and contralateral popliteal nodes of sheep given either a living Staphylococcus aureus vaccine (Group L), a killed S. aureus vaccine with Freund's complete adjuvant (FCA) (Group K) or FCA alone (Group C). The injections were made subcutaneously in the hind legs and second injections were given 4 weeks later in the same site. Very large numbers of IgM-containing cells were found in granulomas of Groups K and C at 1 week post-injection (PI) but by 2 weeks PI only small numbers were recorded. Following booster immunization, the numbers of IgM-containing cells were greater in abscesses from sheep in Group L than from granulomas from sheep in Group K. Levels for Group C remained low after 1 week PI. There was evidence that the very early IgM-containing cell response in Group K was largely unrelated to specific anti-staphylococcal antibody synthesis. The IgG2-containing cell response was significantly greater than the IgG1-containing cell response in abscesses and nodes in Group L. In contrast, for animals in Groups K and C the IgG1-containing cell response predominated over the IgG2-containing cell response in granulomas and draining nodes. There were generally greater numbers of cells containing anti-staphylococcal antibody in abscesses/granulomas and nodes in Group L than in Group K.
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000907:Antibodies, Bacterial; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000276:immunology; D006099:Granuloma / Q000209:etiology / Q000276:immunology / Q000473:pathology; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D008297:Male; D011817:Rabbits; D012756:Sheep; D013203:Staphylococcal Infections / Q000276:immunology*; D013209:Staphylococcal Vaccines / Q000008:administration & dosage* / Q000145:classification / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013209:Staphylococcal Vaccines