Cathodic trypsin-like immunoreactivity in serum: influence of sex, age, renal function, food and diurnal variation.
Radioimmunologically determined cathodic trypsin-like immunoreactivity (TLI) in serum was positively correlated with age (Spearman's rho = 0.40, p less than 0.001) and negatively correlated with estimated creatinine clearance (Spearman's rho = -0.31, p less than 0.01) in healthy adults (37 women, 27 men) under standard conditions. Women had higher TLI concentrations than men (median value 358 micrograms trypsin standard/l compared with 283 micrograms trypsin standard/l, p less than 0.02). A clinically significant uptake of cathodic trypsin from the intestine to the blood is not likely because no change was found in serum concentrations of TLI and the two major protease inhibitors (alpha 1-antitrypsin and alpha 2-macroglobulin) one hour after a standard meal. No diurnal variation of TLI in serum was found in a group of 12 healthy subjects. The results indicate that due consideration must be given to sex, age and renal function of the persons used in reference groups for TLI determination.
D000328:Adult; D000367:Age Factors; D002940:Circadian Rhythm; D003404:Creatinine / Q000378:metabolism; D004563:Electrochemistry; D005260:Female; D005502:Food; D006801:Humans; D007668:Kidney / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay; D012737:Sex Factors; D014357:Trypsin / Q000097:blood*
D003404:Creatinine; D014357:Trypsin