title: Kinetics of primary and secondary stimulation of the mRNA for APOVLDL-II, a major yolk protein, in the cockerel liver by estrogen.
abstract: ApoVLDL-II, the major very low density lipoprotein (VLDL) apoporotein, is also a major yolk protein in the chicken. The response of apoVLDL-II mRNA to estrogen treatment in 5-week-old cockerels was studied by hybridization to a nick-translated probe from cloned apoVLDL-II DNA. Following either primary or secondary stimulation (9 days after primary stimulation) of cockerels with 17 beta-estradiol (5 mg/kg), apoVLDL-II mRNA concentration increased from basal levels of 0.5 and 3.7 molecules/nuclear DNA equivalent respectively by 500- to 1000-fold within 3 h. The concentration of apoVLDL-II mRNA after 3 h during the secondary stimulation reached a level (1800 molecules/cell) 3.6-fold higher than that attained by primary stimulation (500 molecules/cell) at the same time point. Plasma 17 beta-estradiol concentrations following primary and secondary hormone treatment were similar during this time.
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000096:biosynthesis*; D002645:Chickens; D004527:Egg Proteins / Q000096:biosynthesis; D004958:Estradiol / Q000494:pharmacology*; D007700:Kinetics; D008079:Lipoproteins, VLDL / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*
chemical_list: D001053:Apolipoproteins; D004527:Egg Proteins; D008079:Lipoproteins, VLDL; D012333:RNA, Messenger; C027610:apolipoprotein VLDL II; D004958:Estradiol