[Cytochrome P-450 and aryl hydroxylase activity in cells of a long-term transplantable tumor].
A functionally active system of microsomal monooxygenases has been found in a long-term transplanted tumor MC-II of C57B1/6j mice. In microsomal fraction of the tumor, one could detect cytochrome P-450 and benzo(a)pyrene hydroxylase (BP hydroxylase) activity. The latter one increased more than 2 times after the animals received 3-MC and aroclor 1254. In in-vitro experiments, the microsomal monooxygenase inhibitors, SKF 525-A and metyrapone, did not affect BP hydroxylation, whereas alpha-naphthoflavone inhibited the enzyme. It is assumed that tumor MC-II contains hemoprotein that is similar to cytochrome P1-450.
D000818:Animals; D001140:Aroclors; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D001579:Benzopyrene Hydroxylase / Q000378:metabolism*; D020111:Chlorodiphenyl (54% Chlorine); D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D008297:Male; D008748:Methylcholanthrene; D008797:Metyrapone / Q000494:pharmacology; D051379:Mice; D008861:Microsomes / Q000201:enzymology; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000201:enzymology*; D010105:Oxygenases / Q000037:antagonists & inhibitors; D011335:Proadifen / Q000494:pharmacology; D013997:Time Factors
D001140:Aroclors; D020111:Chlorodiphenyl (54% Chlorine); D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System; D011335:Proadifen; D010105:Oxygenases; D001579:Benzopyrene Hydroxylase; D001189:Aryl Hydrocarbon Hydroxylases; D008797:Metyrapone
Tsitokhrom P-450 i aktivnost' arilgidroksilazy v kletkakh dlitel'no perevivaemoĭ opukholi.