The effect of hemin and of allyl isopropyl acetamide on protein synthesis in rat hepatocytes.
Protein synthesis was measured in incubated hepatocytes. While hemin brings about a slight stimulation, allyl isopropyl acetamide (a compound that destroys the heme bound to cytochrome P450) inhibits protein synthesis by a mechanism that appears to result exclusively from depletion of cytoplasmic heme. Indications that in hepatocytes, as in reticulocytes, protein synthesis may be in part regulated by heme at the level of initiation are: i) that inhibition is accompanied by polysome breakdown; ii) that the protein synthesis inhibitor already isolated from rat liver, is hemin reversible iii) that hepatocyte extracts contain a Mr 38,000 phosphoprotein which comigrates with the Mr 38,000 subunit of rabbit initiation factor 2 and iv) that the phosphorylation of both of these subunits is inhibited by hemin.
D000081:Acetamides / Q000494:pharmacology*; D000502:Allylisopropylacetamide / Q000494:pharmacology*; D000818:Animals; D015852:Eukaryotic Initiation Factor-2; D006418:Heme / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D010448:Peptide Initiation Factors / Q000302:isolation & purification; D010750:Phosphoproteins / Q000302:isolation & purification; D010766:Phosphorylation; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects*; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D012156:Reticulocytes / Q000378:metabolism; D014366:Tryptophan Oxygenase / Q000378:metabolism
D000081:Acetamides; D015852:Eukaryotic Initiation Factor-2; D010448:Peptide Initiation Factors; D010750:Phosphoproteins; D011506:Proteins; D000502:Allylisopropylacetamide; D006418:Heme; D014366:Tryptophan Oxygenase