Effect of acclimation temperature on oxygen transport in the blood of the carp, Cyprinus carpio.
The temperature dependence of the O2 equilibrium in whole blood was measured in carp acclimated for more than 3 weeks at 10 degrees C or 20 degrees C water temperature. O2 combining curves were obtained for the same samples at 10 degrees C or 20 degrees C using blood from the 10 degrees C or 20 degrees C acclimated fish (group A) or only at the acclimation temperature (group B). Whereas in group B the P50 was about the same at both temperatures, in group A P50 was higher at 20 degrees C than at 10 degrees C, yielding an apparent heat of oxygenation delta H = -9.9 kcal/mol at pH = 8.0. The CO2 Bohr effect in group A was delta log P50/delta pH = -0.93 at 20 degrees C and -1.17 at 10 degrees C, whereas in group B no temperature effect was seen (-0.98 and -0.97). The acclimation temperature had no effect on the electrophoretic Hb pattern. As expected, the in vivo pH changed inversely with temperature from 8.06 at 10 degrees C to 7.73 at 20 degrees C, enhancing the temperature-induced shift in P50. Acclimation reverses partly the changes in O2 affinity, thereby improving the uptake of oxygen in the gills.
D000064:Acclimatization; D000818:Animals; D002347:Carps / Q000502:physiology*; D003530:Cyprinidae / Q000502:physiology*; D006454:Hemoglobins / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010100:Oxygen / Q000097:blood*; D013696:Temperature
D006454:Hemoglobins; D010100:Oxygen