Stimulation-dependent uptake of an extracellular marker to subcellular fractions of isolated neurohypophysial tissue.
Slices of ox neurohypophyses and groups of isolated rat neurointermediate lobes were incubated in a medium containing horseradish peroxidase (HRP) and stimulated by high K+ concentrations in the medium. After washing, the tissue was homogenized and subjected to subcellular fractionation in a Percoll/sucrose gradient. HRP was exclusively taken up by particles banding at a low density of the gradient. The HRP containing particles located to this region included vacuoles of a size comparable to secretory vesicles.
D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D005260:Female; D006735:Horseradish Peroxidase / Q000378:metabolism; D010904:Pituitary Gland, Posterior / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D011188:Potassium / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000378:metabolism*
D006735:Horseradish Peroxidase; D011188:Potassium