[Cytofluorometric study of the glycogen content in DNA-synthesizing and -nonsynthesizing hepatocytes in the regenerating liver].
Using a combined cytochemical method that allows to determine glycogen, DNA and 3H-thymidine label in the same cell, glycogen amounts were measured both in 3H-TdR-marked and non-marked hepatocytes of the regenerating 3H-thymidine. During mitosis, the glycogen amount is reduced if compared with that in cells being in presynthetic phase. It is proposed that the decrease in glycogen content in the regenerating liver may partly depend on energetic expenses of cells that started DNA synthesis and mitotic division. The phenotypic expression of genes responsible for glycogen synthesis and splitting in di-, tetra- and octoploid hepatocytes of the regenerating liver liver was proportional to the corresponding values.
D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D005434:Flow Cytometry; D006498:Hepatectomy; D006651:Histocytochemistry; D008099:Liver / Q000378:metabolism*; D008112:Liver Glycogen / Q000032:analysis*; D008115:Liver Regeneration; D008297:Male; D010641:Phenotype; D011003:Ploidies; D051381:Rats
D008112:Liver Glycogen; D004247:DNA
Tsitofluorimetricheskoe issledovanie soderzhaniia glikogena v sinteziruiushchikh i ne sinteziruiushchikh DNK gepatotsitakh regeneriruiushcheĭ pecheni.