Chromosome displacement and spindle tubule polymerization: 1. The effect of alterations in pH on displacement frequency.
In order to test whether 'chromosome displacement' (Ford and Lester, 1982) is related to spindle function, the phenomenon was examined under conditions known to alter spindle tubule polymerization, namely alterations in pH within the range 6.8-8.0. The following observations were made: (a) the frequency of cells showing chromosome displacement was not altered by variations in pH, but the number of displaced chromosomes per cell was markedly changed. Minimum numbers of chromosomes were displaced at pH 7.6. (b) For any chromosome, the extent of the response to pH change, was positively correlated with the basal displacement rate for that chromosome. (c) Chromosomes which have a 'stable' evolutionary history have a more predictable response to pH than those which have an 'unstable' history. Since displacement is significantly influenced by pH, it is concluded that the phenomenon is related to spindle structure.
D000328:Adult; D002877:Chromosomes, Human / Q000502:physiology*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008870:Microtubules / Q000378:metabolism*; D008938:Mitosis; D011108:Polymers
D011108:Polymers