Enzymatic synthesis of a 21-nucleotide coat protein binding fragment of R17 ribonucleic acid.
An oligoribonucleotide with a sequence identical with the bacteriophage R17 replicase initiator region has been synthesized. The sequence also encompasses the binding domain of R17 coat protein, which is known to act as a translational repressor at this site. The 21-nucleotide fragment was synthesized entirely by enzymatic methods, T4 RNA ligase being used to join shorter oligomers. The resulting fragment has a secondary structure with the expected thermal stability. Since the synthetic fragment binds R17 coat protein with the same affinity as a 59-nucleotide fragment isolated from R17 RNA, we conclude that it has full biological activity.
D001483:Base Sequence; D003090:Coliphages / Q000201:enzymology; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000235:genetics; D010446:Peptide Fragments; D011485:Protein Binding; D012316:RNA Nucleotidyltransferases / Q000235:genetics*; D012367:RNA, Viral / Q000138:chemical synthesis*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D014764:Viral Proteins / Q000378:metabolism*
D009841:Oligonucleotides; D009843:Oligoribonucleotides; D010446:Peptide Fragments; D012367:RNA, Viral; D014764:Viral Proteins; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase