Binding of monoclonal antibodies to the nuclear estrogen receptor in intact nuclei.
A monoclonal antibody to calf uterus cytoplasmic estrogen receptor shows a specifically displaceable and saturable binding to intact nuclei of mouse uterus after estradiol stimulation. The binding is complete after 3 hr at 0 degree C. The binding of the antibody correlates with the exchangeable estradiol binding activity of the nuclei over a 4-hr time course following in vivo injection of 17 beta-estradiol.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002369:Castration; D002417:Cattle; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D007700:Kinetics; D051379:Mice; D011959:Receptors, Estradiol; D011960:Receptors, Estrogen / Q000276:immunology / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D011959:Receptors, Estradiol; D011960:Receptors, Estrogen; D004958:Estradiol