The preparative separation of sialic acid-containing lipids from sulphate group-containing glycolipids from small intestine of different animals. Analysis by thin-layer chromatography and detection of novel species.
Acidic glycolipids (gangliosides and glycolipid sulphates) were purified from non-glycolipid contaminants by silicic acid chromatography of their acetylated derivatives. Acetylation converted gangliosides into non-polar neutral and weakly acidic derivatives. These were separated from acetylated sulphate-containing glycolipids by chromatography on DEAE-cellulose. The fractions thus isolated from small intestine of 7 different animals were analyzed by thin-layer chromatography. This revealed a considerable species-related variation of both gangliosides and sulphoglycolipids. Mass spectrometry demonstrated a novel ganglioside in guinea-pig small intestine and a novel sulphoglycolipid in mouse small intestine.
D000107:Acetylation; D000818:Animals; D002415:Cats; D002855:Chromatography, Thin Layer; D005260:Female; D005399:Fishes; D005732:Gangliosides / Q000302:isolation & purification; D006017:Glycolipids / Q000302:isolation & purification*; D006168:Guinea Pigs; D007422:Intestines / Q000032:analysis*; D008297:Male; D051381:Rats
D005732:Gangliosides; D006017:Glycolipids; C009255:sialoglycolipids; C011117:sulfoglycolipids