title: The effect of five days' fasting on skeletal muscle enzymes in obese men.
abstract: The effect of 120 hours' (five days) fasting on the activity of some enzymes of energy metabolism in skeletal muscles was investigated in six obese young men. The results revealed a significant decline in the activity of the following enzymes: triosophosphate dehydrogenase (by 20%), glycerol-3-phosphate dehydrogenase (by 24%), lactate dehydrogenase (by 13%), citrate synthase (by 20%), hydroxyacyl-CoA dehydrogenase (by 40%), while the hexokinase and malate dehydrogenase activities were not significantly altered. Contrary to muscles of non-obese healthy men (Vondra, Bass, Brodan, Kuhn, Andĕl, Veselková and Vítek 1982), a smaller decline of activities of the investigated enzymes occurred together with a paradoxical change of the enzyme pattern, namely a predominance of carbohydrate catabolism and a decline of the role of fatty acids in muscle energy metabolism.
mesh_terms: D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D000328:Adult; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004734:Energy Metabolism; D005215:Fasting; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000378:metabolism; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000201:enzymology*; D009765:Obesity / Q000201:enzymology*
chemical_list: D006153:Guanosine Diphosphate; D015094:3-Hydroxyacyl CoA Dehydrogenases; D007770:L-Lactate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D002950:Citrate (si)-Synthase