Tritiated thymidine experiments in the cat: a description of techniques and experiments to define the time-course of radioactive thymidine availability.
Tritiated thymidine [(3H]thymidine) autoradiographic techniques have been used to define birthdates for cells in a variety of animals. In an effort to include [3H]thymidine experiments in our ongoing studies of visual system development, we have used an intrauterine injection procedure that affords [3H]thymidine labeling of dividing nerve cells in the cat. This report contains a detailed description of the injection procedures used, as well as the results of experiments undertaken to define the period of time during which the exogenous [3H]thymidine introduced by such injections remains available for uptake.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D002415:Cats; D002454:Cell Differentiation; D005260:Female; D005829:Geniculate Bodies / Q000196:embryology*; D005865:Gestational Age; D008938:Mitosis; D009474:Neurons / Q000166:cytology; D011247:Pregnancy; D013787:Thalamic Nuclei / Q000196:embryology*; D013936:Thymidine / Q000378:metabolism; D014795:Visual Pathways / Q000196:embryology
D013936:Thymidine