A method for the purification of placental protein 5 (PP5) from placental extracts.
We describe a method for the preparation of purified PP5 suitable for use as a tracer ligand in RIA. The method is based on the observation that PP5 will bind to heparin and employs a heparin-Sepharose column as a key step in the procedure.
D002845:Chromatography / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D006023:Glycoproteins; D006801:Humans; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000302:isolation & purification*; D011863:Radioimmunoassay / Q000379:methods; D014020:Tissue Extracts / Q000032:analysis*
D006023:Glycoproteins; D011257:Pregnancy Proteins; D014020:Tissue Extracts; C021240:tissue-factor-pathway inhibitor 2