An 'affinity' method for preparing polypeptides enriched in the collagen-associated Ehrlich chromogen.
The collagen-associated compound which reacts at room temperature with acid dimethylaminobenzaldehyde (Ehrlichs reagent) also reacts in acid with aryl-diazonium reagents. We have developed a convenient method for isolating polypeptides associated with the Ehrlich chromogen from collagen digests utilizing diazotized arylamino-cellulose supports. These peptides, in which the Ehrlich chromogen is 'labeled' with yellow diazo color, constitute less than 0.5% of the collagen, and have amino acid patterns similar to those around the cross link regions. The chromogen is not identical with pyridinoline, also thought to be a polyvalent cross link.
D000818:Animals; D001391:Azo Compounds; D001547:Benzaldehydes; D002417:Cattle; D002482:Cellulose; D002863:Chromogenic Compounds; D003094:Collagen / Q000032:analysis*; D010455:Peptides / Q000302:isolation & purification*
D001391:Azo Compounds; D001547:Benzaldehydes; D002863:Chromogenic Compounds; D010455:Peptides; D002482:Cellulose; D003094:Collagen; C510241:p-dimethylaminobenzaldehyde