Effect of cis-dichlorodiammine platinum(II) on the agglutinability of tumor and normal cells with concanavalin A and wheat germ agglutinin.
In this paper we report the effect of cis-dichlorodiammine platinum(II) (cis-platin) on the agglutination of normal and transformed lymphocytes with lectins conconavalin A (Con A) and wheat germ agglutinin (WGA) which is a sensitive test to study the expression of cell surface components. Normal splenocytes showed small degree of cell agglutination with Con A and WGA. This cell agglutination of normal splenocytes with these lectins increases significantly after cis-platin treatment at 37 degrees C. The Dalton's lymphoma cells which show a high degree of cell agglutination with Con A and WGA when treated with cis-platin at 37 degrees C results in decrease in the degree of their cell agglutination with these lectins. Agglutination inhibition studies with alpha-methyl-D-glucopyranoside for Con A and N-acetyl-D-glucosamine for WGA shows that this increase and decrease of cell agglutination after platinum treatment of cells is a specific reaction and not non-specific. Incubation of cis-platin treated normal splenocytes with excess of neuraminic acid results in decrease of cell agglutination with both Con A and WGA. cis-Platin treatment of the cells results in the release of sialic acid from the surface of the cells. A correlation between the release of sialic acid from the cell surface and cell agglutination is also discussed.
D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects*; D002462:Cell Membrane / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects*; D008223:Lymphoma / Q000188:drug therapy*; D051379:Mice; D008811:Mice, Inbred DBA; D009374:Neoplasms, Experimental / Q000188:drug therapy; D011975:Receptors, Mitogen / Q000187:drug effects; D012794:Sialic Acids / Q000378:metabolism; D014909:Wheat Germ Agglutinins
D037102:Lectins; D011975:Receptors, Mitogen; D012794:Sialic Acids; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A; D002945:Cisplatin