Hematologic effects of hemoglobin solutions in animals.
Hb could cause abnormalities in coagulation if stromal lipid contaminated the solution. We prepared Hb by two procedures; it was lipid-free by the assays employed. These solutions were given to three species of animals (dogs, pigs, primates) at the dose of 15 ml/kg and then observations were made for hematologic changes. Only dogs demonstrated significant alterations. A consistent transient thrombocytopenia (60% drop) was seen five minutes after infusion and returned to baseline by one hour. Control dogs, receiving albumin, also showed a transient thrombocytopenia but not as pronounced (15% drop). Two Hb-treated dogs had signs of subclinical DIC (positive FDF, protamine sulfate precipitation, and a 70% drop in Factor VIII). There were no differences in any hematologic parameters between Hb and albumin treated pigs and monkeys. These results show that species-specific hematologic responses to lipid-poor Hb can be demonstrated.
D000818:Animals; D001769:Blood / Q000187:drug effects*; D001772:Blood Cell Count; D001778:Blood Coagulation Disorders / Q000139:chemically induced; D001779:Blood Coagulation Factors / Q000032:analysis; D001792:Blood Platelets / Q000187:drug effects; D004285:Dogs; D006454:Hemoglobins / Q000633:toxicity*; D006801:Humans; D012709:Serum Albumin / Q000494:pharmacology
D001779:Blood Coagulation Factors; D006454:Hemoglobins; D012709:Serum Albumin