Investigation of effects of some immunoadjuvants and routes of antigen administration in raising antibodies in rabbits against metacestode cyst antigens of Taenia multiceps.
The relative efficiency of depot (Freund's complete adjuvant (FCA) and saponin), and non-depot (Corynebacterium parvum strain P. acnes I, C. parvum strain IMI585, and levamisole) immunoadjuvants were compared in raising agglutinating and precipitating antibodies against the metacestode fluid of Taenia multiceps of sheep origin (TMCF). Haemagglutinating but not precipitating antibodies were detectable during the period of primary immunisation. Precipitating antibodies of higher titre were produced for longer when TMCF was injected with immunoadjuvant than when the same volume of TMCF was injected alone. Significantly (P less than 0.05) higher indirect haemagglutination (IHA) titres were produced during the primary response following C. parvum treatment than after FCA treatment. FCA treated rabbits showed significantly (P less than 0.05) higher IHA titres than those receiving C. parvum treatment on day 42 only, during the anamnestic response. Ulcers formed at the sites of injection of depot immunoadjuvants. Significantly (P less than 0.05) higher IHA titres were produced when FCA with TMCF was injected intradermally into 10-12 sites than when the same volume was injected into 4 sites. C. parvum strain IM1585 was a better immunoadjuvant in this system than the strain P. acnes I. The intravenous route of administration yielded better titres than the intraperitoneal route when C. parvum strain P. acnes I was used.
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000372:Agglutination Tests; D000818:Animals; D000917:Antibody Formation; D000936:Antigen-Antibody Complex; D000941:Antigens / Q000008:administration & dosage*; D011817:Rabbits / Q000276:immunology; D013621:Taenia / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000936:Antigen-Antibody Complex; D000941:Antigens