Pharmacokinetics of hemoglobin infusion.
Although research with acellular Hb solutions has been conducted for many years, published data concerning the pharmacokinetics of Hb are quite variable. Preliminary research in this Division has attempted to establish an adequate pharmacokinetic model for SFH and for a modified Hb product. Three dose levels of SFH (55 mg/kg, 111 mg/kg, and 1400 mg/kg were given by rapid (less than 10 min.) intravenous injection to different groups of dogs. Serial blood samples were obtained from indwelling intravenous catheters and plasma Hb levels were measured. Analysis of the plasma SFH concentration-time data showed a first order decline at all three dose levels with no significant (p less than 0.05) difference in elimination rate constants. A significant difference in the volume of distribution at each dose was seen which suggested greater tissue deposition of Hb at higher doses. Consideration of the normalized AUC suggested a non-linear elimination process at high doses. This process appears to be consistent with known Hb elimination mechanisms. A single dose of PPHb was also studied. Following a dose of 650 mg/kg, the plasma Hb levels decline was characterized by a single compartment model with Michaelis-Menten elimination. The model parameters calculated are preliminary and further work is needed to evaluate the model.
D000818:Animals; D004285:Dogs; D006454:Hemoglobins / Q000378:metabolism*; D006461:Hemolysis; D007700:Kinetics; D008297:Male
D006454:Hemoglobins