Apparent species restriction in the isolation of proliferating embryonal carcinoma cell hybrids.
Proliferating somatic cell hybrids are readily obtained from the fusion of a metabolic co-operation-defective mouse embryonal carcinoma cell variant (R5/3) to LMTK- (1 fibroblastic mouse line derived from L-M) and to derivatives of the R5/3 parental cell line PC13. However it has not yet proved possible to produce growing hybrids from fusions between R5/3 and any non-mouse cell line. It appears, from both this work and that of others that although polyethylene glycol-induced fusion occurs between embryonal carcinoma cells and other cell types viable hybrids cannot always be isolated.
D000818:Animals; D002455:Cell Division; D002459:Cell Fusion; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology*; D051379:Mice; D009374:Neoplasms, Experimental / Q000473:pathology; D013045:Species Specificity; D013724:Teratoma / Q000473:pathology*