Isolation, purification and identification of a serum DNA binding protein which increases in malignant diseases.
A DNA binding protein in human serum was purified and identified. This protein was isolated from pooled human serum by DEAE Sephadex column chromatography, DNA cellulose affinity column chromatography, Sephadex G-200 gel filtration and Sepharose 6B gel filtration. It was found that the purified protein consisted of two subunits, one with a molecular weight of 80,000 and the other 25,000, evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The purified protein was in fact IgM determined by physicochemical and immunochemical characteristics.
D002352:Carrier Proteins / Q000097:blood*; D004268:DNA-Binding Proteins; D006801:Humans; D007075:Immunoglobulin M / Q000302:isolation & purification; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000097:blood*; D009369:Neoplasms / Q000097:blood*
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D007075:Immunoglobulin M; D009363:Neoplasm Proteins