[Analysis, by lectin fluorescence, of the cell surface of embryonal fibroblasts cultivated in vitro].
Cultured fibroblast derived from 7 and 14 days chick embryonic skin were tested with three fluorescent lectins (Con A, WGA and SBA). The result obtained shown that the percentage of cells that bind WGA decrease from 7 to 14 days, supporting the presence of an age-dependent heterogeneity at cell surface level. In both cellular population only the 50% of the cell bind to SBA, suggesting a more subtle heterogeneity into the same population.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D005347:Fibroblasts / Q000166:cytology*; D005453:Fluorescence; D006651:Histocytochemistry; D037102:Lectins / Q000378:metabolism*; D008722:Methods; D013499:Surface Properties
D037102:Lectins
Analisi, per mezzo di lectine fluorescenti, della superficie cellulare di fibroblasti embrionali coltivati in vitro.