Fusion of dipalmitoylphosphatidylcholine vesicles at 4 degrees C.
Small sonicated dipalmitoylphosphatidylcholine vesicles when incubated at 4 degrees C and high concentrations are shown to fuse completely to vesicles about 700-A diameter in 7 days, and these further fuse to about 950 A diameter vesicles after 3-4 weeks. The 950 A diameter vesicles are spherical, homogeneous, mostly unilamellar, have an internal aqueous space about 10 times that of small vesicles, and are stable for at least 6 months. The 950-A vesicles are characterized by agarose gel chromatography, freeze-fracturing electron microscopy, trapped volume measurements, differential scanning calorimetry, and diphenylhexatriene fluorescence polarization.
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003080:Cold Temperature; D004355:Drug Stability; D005454:Fluorescence Polarization; D005614:Freeze Fracturing; D008081:Liposomes; D008854:Microscopy, Electron; D011663:Pulmonary Surfactants; D013816:Thermodynamics
D008081:Liposomes; D011663:Pulmonary Surfactants