Occurrence and role of phosphoenolpyruvate carboxykinase in procyclic Trypanosoma brucei brucei glycosomes.
Phosphoenolpyruvate carboxykinase (EC 4.1.1.32) was detected in a particulate fraction of Trypanosoma brucei brucei procyclic culture form. It requires ADP rather than GDP for activity in the direction of carboxylation and is located in the glycosomes. Since phosphoenolpyruvate can serve to furnish ATP for glycolysis and can promote 3-phosphoglycerate or 1,3-bisphosphoglycerate formation without simultaneous alpha-glycerophosphate production, we suggest that the glycosomal phosphoenolpyruvate carboxykinase-malate dehydrogenase tandem contributes to ATP regeneration and NADH re-oxidation in the glycosome, and regulates alpha-glycerophosphate production.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D002500:Centrifugation, Isopycnic; D006019:Glycolysis; D006153:Guanosine Diphosphate / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010728:Phosphoenolpyruvate / Q000378:metabolism; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000201:enzymology*
D009243:NAD; D006153:Guanosine Diphosphate; D000244:Adenosine Diphosphate; D010728:Phosphoenolpyruvate; D010729:Phosphoenolpyruvate Carboxykinase (GTP)