Enhancement of the repair of carcinogen-induced DNA damage in the hamster pancreas by dietary selenium.
We measured single strand breaks (SSB) in pancreas DNA produced by N-nitrosobis (2-oxopropyl)amine (BOP) in hamster fed purified diets containing added sodium selenite (Se) at 0.0, 0.1 and 5.0 ppm. There were fewer SSB in those given the 5.0 ppm Se diet throughout the experiment. One hour after dosing with BOP (20 mg/kg), there were 2.26 +/- 0.47, 2.83 +/- 0.43 and 1.74 +/- 0.43 SSB per 10(8) daltons (mean +/- S.E.M.) respectively in the three groups. The SSB were repaired faster in the 5.0 ppm Se-fed group. The approximate half-lives of the SSB were 33, 30 and 8 days, respectively. In the hamsters fed 5.0 ppm Se there was a small, statistically significant increase in pancreatic DNA synthesis. Autoradiographic analysis indicated that this was repair synthesis. In a second experiment, hamsters were fed one of the three diets prior to and for 2 days after administration of a single dose of BOP (20 mg/kg). They were then fed the 5.0 ppm Se diet for 5 days. The number of SSB was compared with those in hamsters fed their original diet for 7 days after BOP dosing. There was a statistically significant difference in the number of SSB in the hamsters fed 0.1 ppm Se before and for 2 days after BOP. In these hamsters there were 1.21 +/- 0.24 SSB per 10(8) daltons compared with 3.19 +/- 0.4 (mean +/- S.E.M.). These results suggest high levels of dietary Se stimulate the repair of carcinogen-induced DNA damage
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology; D006224:Cricetinae; D004260:DNA Repair / Q000187:drug effects*; D004277:DNA, Single-Stranded / Q000378:metabolism; D004032:Diet; D008297:Male; D008647:Mesocricetus; D009602:Nitrosamines / Q000494:pharmacology*; D010179:Pancreas / Q000187:drug effects; D020887:Selenious Acid; D012643:Selenium / Q000494:pharmacology*
D002273:Carcinogens; D004277:DNA, Single-Stranded; D009602:Nitrosamines; C013622:nitrosobis(2-oxopropyl)amine; D020887:Selenious Acid; D012643:Selenium