title: Effects of dilazep on fibrinolytic system in animals. II. Enhancing effect of dilazep on plasminogen activator release in the isolated perfused pig ear.
abstract: The influence of drugs on the release of plasminogen activator was studied in the isolated perfused pig ear. The pig ear was perfused with oxygenated Tyrode's solution pH 7 . 4, at 37 degrees C via main artery and perfusate from the veins was collected at 2 min intervals. The drug was injected into a rubber tube connected in the front of arteria cannula, and the fibrinolytic activity of perfusate collected was measured by the fibrin plate method using plasminogen-containing bovine fibrinogen. Under the test condition, dilazep enhanced plasminogen activator release (PA release) in a dose-dependent fashion ranging 10 to 100 microgram, however, it did not affect the perfusion pressure. Hypotensors, namely acetylcholine (0.1-3 microgram), bradykinin (0.1-3 microgram) and histamine (0.1-3 microgram) enhanced also the PA release in a dose-dependent fashion with no effects on perfusion pressure. Vasoconstricting drugs, namely phenylephrine (0.3-10 microgram), norepinephrine (0.1-3 microgram) and serotonin (0.1-3 microgram) exerted hypertensive effects on perfusion pressure in a dose-dependent fashion, however, it did not cause the PA release.
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D001381:Azepines / Q000494:pharmacology*; D001920:Bradykinin / Q000378:metabolism; D004109:Dilazep / Q000494:pharmacology*; D004176:Dipyridamole / Q000494:pharmacology; D004431:Ear, External / Q000378:metabolism; D005342:Fibrinolysis / Q000187:drug effects*; D006632:Histamine / Q000378:metabolism; D066298:In Vitro Techniques; D010960:Plasminogen Activators / Q000378:metabolism*; D013552:Swine; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D001381:Azepines; D014665:Vasodilator Agents; D004176:Dipyridamole; D006632:Histamine; D010960:Plasminogen Activators; D004109:Dilazep; D000109:Acetylcholine; D001920:Bradykinin