Deacetylation of PS-5, a new beta-lactam compound, I. Microbial deacetylation of PS-5.
PS-5 was deacetylated to NS-5 (deacetylated PS-5) by l-amino acid acylase from porcine kidney and D-amino acid acylase from Streptomyces olivaceus but not by l-amino acid acylase from Aspergillus sp. Using PS-5, N-chloroacetyl-l-phenylalanine and N-chloroacetyl-D-valine as substrates, acylase producers were screened among facultative methanol-assimilating bacteria. Most of the microbes tested were active and could be classified into two groups of l-acylase producers and L-& D-acylase producers. Pseudomonas sp. 1158 which deacetylated the three substrates was chosen for further study. Cells of the bacterium entrapped in polyacrylamide gel and its acylase activities immobilized on DEAE-Sephadex were found to be useful for conversion of PS-5 to NS-5.
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001230:Aspergillus / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D011549:Pseudomonas / Q000201:enzymology; D013302:Streptomyces / Q000201:enzymology; D013552:Swine; D013845:Thienamycins; D047090:beta-Lactams / Q000378:metabolism
D000900:Anti-Bacterial Agents; D013845:Thienamycins; D047090:beta-Lactams; C016883:PS 5; D000217:Acyltransferases