title: Calcium-mediated changes in related malignant and nonmalignant mouse epidermal cell lines.
abstract: Two epidermal cell lines of common origin, one tumorigenic and the other not, have been grown in media of different Ca2+ concentrations (low: 0.07 mM; normal: 1.4 mM; high: 10 mM). At normal Ca2+ levels, both cell lines had similar growth rates. The growth rates were reduced at both higher and lower Ca2+ concentrations, with greater inhibition of the tumorigenic than the nontumorigenic cells. At a given level of Ca2+, the 2 cell lines could not be distinguished in their polyacrylamide gel patterns by protein staining, by [125I]concanavalin A reactivity, and by lactoperoxidase iodination. Changes in surface membrane proteins with Ca2+ concentrations were observed by lactoperoxidase iodination, whereas the other techniques, which measure total cell proteins or glycoproteins, showed no substantial variation.
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D004817:Epidermis / Q000187:drug effects*; D006023:Glycoproteins / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D012878:Skin Neoplasms / Q000473:pathology*
chemical_list: D006023:Glycoproteins; D003208:Concanavalin A; D002118:Calcium