title: Isolation of cofactor common to molybdenum-containing enzymes: nitrate reductase from lupine bacteroids and xanthine oxidase from milk.
abstract: A quantitative method for the anaerobic isolation of a molybdenum cofactor from two molybdenum-containing enzymes, nitrate reductase from the bacteroids of lupine nodules and xanthine oxidase from milk, is described. It was established that the cofactor consists of an aromatic component and a number of amino acid residues bound to it. The structural and catalytic function of the molybdenum cofactor in the enzyme was established.
mesh_terms: D000818:Animals; D003067:Coenzymes / Q000302:isolation & purification*; D005260:Female; D008892:Milk / Q000201:enzymology*; D008982:Molybdenum / Q000302:isolation & purification*; D009565:Nitrate Reductases / Q000032:analysis*; D012231:Rhizobium / Q000201:enzymology*; D014969:Xanthine Oxidase / Q000032:analysis*
chemical_list: D003067:Coenzymes; D008982:Molybdenum; D014969:Xanthine Oxidase; D009565:Nitrate Reductases