title: The ability of the rat epididymis to concentrate spermatozoa. Responsiveness to aldosterone.
abstract: Experiments have been performed to determine if aldosterone is involved in the control of water reabsorption from the epididymal lumen in vivo. Micropuncture samples of lumen content were collected from the epididymides of control rats and those receiving aldrenalectomy, adrenalectomy + 25 micrograms aldosterone/day, 10 mg spironolactone/kg body weight/day, 10 mg spironolactone + 1 mg testosterone/kg body weight/day, 5 mg desoxycorticosterone acetate (DOCA)/day, 50 micrograms aldosterone/day, or 0.1 ml vehicle alone. The treatment period was three days. Seminal vesicles weights and testis weights were obtained. Sperm concentrations (SEM) in the caput, corpus, and cauda epididymidis of normal rats were 0.75 +/- 0.05, 1.24 +/- 0.13, and 1.99 +/- 0.15 x 10(9) sperm/ml, respectively. Both inhibition and removal of aldosterone caused significant reduction (P less than .01) of intraluminal sperm concentrations. Sham treatment had no effect. Sperm concentrations were normal in animals receiving aldrenalectomy plus aldosterone replacement. It is concluded that water resorption in the rat epididymis is responsive to aldosterone.
mesh_terms: D000315:Adrenalectomy; D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D003900:Desoxycorticosterone / Q000494:pharmacology; D004822:Epididymis / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013094:Spermatozoa / Q000502:physiology*; D013148:Spironolactone / Q000494:pharmacology
chemical_list: D013148:Spironolactone; D003900:Desoxycorticosterone; D000450:Aldosterone