High performance liquid chromatography and UV detection for the separation and quantitation of prostaglandins.
A fast and reliable method for the separation and quantitation of arachidonic acid metabolites PGF1 alpha, PGF2 alpha, PGD2, PGE1, PGE2, PGB2, PGA2, 6-keto PGE1, 6-keto PGF1 alpha, TxB2 and 15-keto PGE2 by high-performance liquid chromatography has been developed. Utilizing a single reverse-phase column and a UV spectrophotometer, sensitivity as little as 30 nanograms of each of these prostaglandins can be separated and subsequently detected. Although this study was performed using standards, it is highly promising for future application to biological fluids.
D015121:6-Ketoprostaglandin F1 alpha; D000527:Alprostadil / Q000031:analogs & derivatives*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015232:Dinoprostone / Q000031:analogs & derivatives*; D011453:Prostaglandins / Q000032:analysis*; D011454:Prostaglandins A / Q000032:analysis; D011456:Prostaglandins B / Q000032:analysis; D011457:Prostaglandins D / Q000032:analysis; D011458:Prostaglandins E / Q000032:analysis; D011460:Prostaglandins F / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013929:Thromboxane B2 / Q000032:analysis
D011453:Prostaglandins; D011454:Prostaglandins A; D011456:Prostaglandins B; D011457:Prostaglandins D; D011458:Prostaglandins E; D011460:Prostaglandins F; C026346:15-ketoprostaglandin E2; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; C022950:6-ketoprostaglandin E1; D000527:Alprostadil; D015232:Dinoprostone