[Incorporation of adenine derivatives into the cells of Brevibacterium ammoniagenes ganA mutants producers of inosinic acid].
The ganA mutants (growth ability with nucleotides), overproducers of inosinic acid, were isolated with the aid of specially developed techniques aimed at obtaining mutants with the increased ability of assimilating adenine derivatives. The selection techniques used, as well as certain properties of ganA mutants distinguishing them from their parent strains auxotropic for adenine, led to suggesting that ganA mutation affects cell permeability for exogenous adenine derivatives. The present paper reports on a comparative study of two ganA mutants and their parent strains regarding their ability to grow on a minimal medium supplemented with various concentrations of adenine, adenosine on adenylic acid. The mutants grew better than the initial strains at concentrations of adenine or adenosine ranging from 0.005 to 0.05 mM and at a concentration of adenylic acid equal to 0.5 mM or higher. The enhanced ability of ganA mutants of assimilate exogenous adenine sources was found to correlate with an increased rate of 14C-adenine and 3H-adenosine uptake by cells. The results indicate that the ganA mutation leads to an increased permeability of adenine-dependent Brevibacterium ammoniagenes cells for adenine derivatives and suggest a participation of this gene in the formation of the cell envelope.
D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism; D000241:Adenosine / Q000378:metabolism; D001951:Brevibacterium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D007291:Inosine Monophosphate / Q000096:biosynthesis*; D007292:Inosine Nucleotides / Q000096:biosynthesis*; D009154:Mutation; D012641:Selection, Genetic
D003470:Culture Media; D007292:Inosine Nucleotides; D007291:Inosine Monophosphate; D000225:Adenine; D000241:Adenosine
Vkliuchenie proizvodnykh adenina v kletki mutantov Brevibacterium ammoniagenes ganA--produtsentov inozinovoĭ kisloty.