[Spectrofluorimetric procedures for estimation of lysosomal cathepsins A, B C and D activities].
Unified modifications of procedures are described for estimation of lysosomal cathepsins A, B, C and D activities. The procedures are based on spectrofluorimetric determination of beta-naphthylamine in the experiments with cathepsins B and C or on spectrofluorimetric determination of fluorescamine conjugates with epsilon-NH2-groups of free amino acids in the case of cathepsins A and D. Kinetics of all the reactions studied obeyed the Michaelis-Menten equation; the enzymatic activities could be estimated at protein concentration up to 0.1 mg/ml of the experimental sample.
D000818:Animals; D002268:Carboxypeptidases / Q000378:metabolism; D043402:Cathepsin A; D002401:Cathepsin B; D002402:Cathepsin D; D002403:Cathepsins / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology*; D051381:Rats; D013050:Spectrometry, Fluorescence / Q000379:methods
D002268:Carboxypeptidases; D002403:Cathepsins; D043402:Cathepsin A; D002401:Cathepsin B; D002402:Cathepsin D
Spektrofliuorimetricheskie metody opredeleniia aktivnosti lizosomnykh katepsinov A, B, C i D.