title: Vitamin D uptake by the perfused rat liver is determined by its transport protein.
abstract: Radiolabelled vitamin D3 and 25(OH)vitamin D3 (25(OH)D) were added to an isolated perfused rat liver, bound either to lipoproteins or lipoprotein-free plasma. The disappearance of vitamin D and 25(OH)D from the perfusate was determined. When vitamin D or 25(OH)D were added to the perfusate on lipoproteins there was a rapid decline in the amount present in the perfusate, as compared to when they were added bound to lipoprotein-free plasma. The uptake of vitamin D and 25(OH)D by the liver from lipoproteins was associated with a transfer of radioactivity from the lipoprotein portion to the lipoprotein-free portion of the perfusate. These results demonstrate the importance of the vitamin D transport protein to its uptake by the liver.
mesh_terms: D000818:Animals; D002112:Calcifediol / Q000378:metabolism; D002352:Carrier Proteins / Q000502:physiology*; D066298:In Vitro Techniques; D008074:Lipoproteins / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D051381:Rats; D014807:Vitamin D / Q000378:metabolism*; D014809:Vitamin D-Binding Protein
chemical_list: D002352:Carrier Proteins; D008074:Lipoproteins; D014809:Vitamin D-Binding Protein; D014807:Vitamin D; D002112:Calcifediol