Effects of delayed amputation on denervated forelimbs of adult newt.
Left forelimbs of adult newts were repeatedly denervated prior to and following amputation. Limb amputations were performed at 7-, 14-, 21-, 27-and 45-day intervals after the initial denervation. Regeneration was found in the sham-denervated and control animals but did not occur in any of the experimental cases; instead cicatrix formation and dermal wound healing ensued. Soft-tissue dedifferentiation was evident, however. We conclude from these results that forelimb regeneration in the adult newt is completely nerve dependent (for growth).
D000671:Amputation, Surgical; D000818:Animals; D002454:Cell Differentiation; D002921:Cicatrix; D003714:Denervation; D005552:Forelimb / Q000294:innervation / Q000502:physiology*; D009673:Notophthalmus viridescens; D012038:Regeneration; D013997:Time Factors; D014945:Wound Healing