Analysis of rat liver Golgi apparatus proteins and apolipoproteins in acute ethanol intoxication.
Protein patterns of rat liver secretive (F1, F2) and formative (F3) Golgi fractions and their corresponding VLDLs content have been analyzed by disc-electrophoresis in normal conditions and after acute ethanol intoxication and compared with serum proteins and isolated lipoproteins. The major bands of the normal nascent VLDLs isolated from both formative and secretive Golgi fractions migrate in the same area of serum apo-C and apo-ARP apolipoproteins; in the same density interval of VLDLs another major lipoprotein band, called U, may be separated showing a slower electrophoretic mobility of apo-ARP. After ethanol intoxication apo-C bands seem to be concentrated in secretive and decreased in formative fractions, while apo-ARP and U bands show an overlapping in both Golgi fractions. The possible mechanisms of these behaviours are discussed.
D000435:Alcoholic Intoxication / Q000150:complications / Q000378:metabolism*; D000818:Animals; D001053:Apolipoproteins / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis*; D004589:Electrophoresis, Disc; D005235:Fatty Liver, Alcoholic / Q000209:etiology / Q000378:metabolism*; D005260:Female; D006056:Golgi Apparatus / Q000032:analysis*; D006801:Humans; D008075:Lipoproteins, HDL / Q000032:analysis; D008079:Lipoproteins, VLDL / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000032:analysis
D001053:Apolipoproteins; D001798:Blood Proteins; D008075:Lipoproteins, HDL; D008079:Lipoproteins, VLDL