[Determination of the membrane fluidity of human blood platelets by measuring the anisotrophy of the fluorescence emission].
Incorporation in vitro of 1,6-diphenyl-1,3,5 hexatriene (DPH) in washed human blood platelets was studied to determine a reference value of fluorescence emission anisotropy of DPH and thus the "microfluidity " of platelet lipid zones. Parameters evaluated were the effect of time of incorporation of the marker, of temperature (25 and 37 degrees C), and of cellular concentration, as well as the effect of platelet aging, enabling determination of valid experimental conditions for studying interaction with drug molecules.
D001792:Blood Platelets / Q000032:analysis*; D002470:Cell Survival; D004161:Diphenylhexatriene; D005454:Fluorescence Polarization / Q000379:methods; D006801:Humans; D008560:Membrane Fluidity; D010976:Platelet Count; D013696:Temperature; D013997:Time Factors
D004161:Diphenylhexatriene
Détermination de la fluidité de la membrane des plaquettes sanguines humaines par mesure de l'anisotropie de l'émission de fluorescence.