A contractile ring-like mechanism in wound healing and soluble factors affecting structural stability in the cortex of Xenopus eggs and oocytes.
Holes or tears have been made in the surface of unfertilized Xenopus eggs or oocytes in various buffered media and the surface response to the wound has been observed with the light microscope. In the presence of calcium ion in the medium, a pigmented ring appears around the wound and constricts in purse-string fashion until the hole is closed. A highly pigmented scar remains. We show that the surrounding pigmented cortex is moved over the denuded cytoplasm when wound healing closes a large excision wound in the pigmented surface. This movement of surface is consistent with a model of a purse string, closing the hole by pulling existing surface over the denuded cytoplasm. The presence of cytochalasin B in the medium inhibits wound healing completely. The sensitivity to the antibiotic is similar to that of the contractile ring in cytokinesis. Wounds made in the surface of ripe ovarian oocytes do not heal in the presence of calcium ion. The healing mechanisms, or their sensitivity to calcium, thus appear during meiotic maturation or ovulation. The egg cortex and membrane around wounds dissociate in the absence of calcium or in the presence of cytochalasin B. The cell cortex of oocytes also dissolves around wounds even in the presence of calcium. This cortical dissolution will not occur in the isolated cortex and thus requires soluble cytoplasmic factors. Models are proposed to explain these observations.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D003571:Cytochalasin B / Q000494:pharmacology; D005260:Female; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D010063:Ovum / Q000187:drug effects / Q000502:physiology*; D010858:Pigmentation; D014945:Wound Healing; D014982:Xenopus laevis
D003571:Cytochalasin B; D002118:Calcium