title: Interaction of the brush-border hydrolases of the human small intestine with lectins.
abstract: The role of human intestinal brush-border membrane hydrolases in lectin binding to the apical region of the enterocyte was investigated. The crossed-immunoelectrophoresis method was used to identify six hydrolases before and after interaction with nine lectins. Three lectins (Phaseolus vulgaris, Ulex europeus, and Ricinus communis 120 agglutinins) bound the hydrolases of 5 different subjects. Helix pomatia agglutinin interacted with only 1 subject.
mesh_terms: D002462:Cell Membrane / Q000201:enzymology*; D003208:Concanavalin A / Q000378:metabolism; D006801:Humans; D006867:Hydrolases / Q000032:analysis / Q000378:metabolism*; D007123:Immunoelectrophoresis, Two-Dimensional; D007421:Intestine, Small / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D008871:Microvilli / Q000201:enzymology*; D010835:Phytohemagglutinins / Q000378:metabolism; D011485:Protein Binding; D011975:Receptors, Mitogen / Q000378:metabolism
chemical_list: D037102:Lectins; D010835:Phytohemagglutinins; D011975:Receptors, Mitogen; D003208:Concanavalin A; D006867:Hydrolases