[Behavior of hypophyseal hormones in leukocytes of normal and leukemic subjects. Radio-immunologic study on intact cells].
The behaviour of GH, LH and TSH was investigated in peripheral leukocytes from normal subjects and patients with acute and chronic myeloid and lymphatic leukaemia. A double-antibody radioimmunological method was used to examine intact cells, after separation of granulocytes from lymphocytes on a Ficoll-Hypaque gradient. The results obtained showed a higher concentration of LH in normal granulocytes by comparison with lymphocytes, and a marked fall in all (except chronic lymphatic) leukaemias. GH and TSH, on the other hand, were higher in normal lymphocytes as opposed to granulocytes, while GH was reduced in all forms of leukaemia and TSH slightly increased (except in CLL). In addition, TSH was found in smaller quantities than the other hormones in all cell types examined. The differences between leukaemic and normal cells are tentatively explained by postulating a change in membrane receptors during the leukaemic transformation of blood cells.
D013006:Growth Hormone / Q000032:analysis*; D006801:Humans; D007938:Leukemia / Q000276:immunology; D007962:Leukocytes / Q000032:analysis* / Q000276:immunology; D007986:Luteinizing Hormone / Q000032:analysis*; D011863:Radioimmunoassay; D013972:Thyrotropin / Q000032:analysis*
D007986:Luteinizing Hormone; D013972:Thyrotropin; D013006:Growth Hormone
Comportamento di alcuni ormoni ipofisari nei leucociti di soggetti normali e leucemici. Determinazione radio-immunologica su cellule integre.