A novel glycoasparagine isolated from an ovalbumin glycopeptide fraction (GP-IV). Anion-exchange borate chromatography and structural analysis of GP-IV glycoasparagines.
The fraction GP-IV obtained from hen ovalbumin has been further fractionated on DEAE-Sephadex A-25 in borate buffer, a new system for the separation and isolation of glycoasparagines on columns. The structures of one major and two minor glycoasparagines thus obtained were determined by enzymatic (endo-beta-N-acetylglucosaminidase D and H digestion), chemical (modified Smith degradation, acetolysis, and methylation analysis), and instrumental (270 MHz 1H nuclear magnetic resonance spectroscopy) analyses. The following structure is novel for carbohydrate moieties in ovalbumin glycoasparagines: (formula; see text) The present anion exchange chromatography in borate solution is well suited for separating and preparing oligosaccharides having similar molecular size but differing in content of vicinal cis-diol group.
D000818:Animals; D001881:Borates; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens; D002852:Chromatography, Ion Exchange; D004529:Egg White / Q000032:analysis; D006020:Glycopeptides / Q000032:analysis* / Q000302:isolation & purification*; D010047:Ovalbumin / Q000032:analysis*
D001881:Borates; D006020:Glycopeptides; C007872:glycoasparagines; D010047:Ovalbumin