mRNA sequences define an unusually restricted IgG response to 2-phenyloxazolone and its early diversification.
Light and heavy chain mRNAs of 15 anti-oxazolone antibodies were sequenced directly using synthetic primers. Hybridomas obtained 7 days after primary immunization show that they are of restricted heterogeneity. The majority expressed a single sequence with minor differences concentrated in the D region and joining boundaries D--JH and V kappa--J kappa.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D001483:Base Sequence; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010080:Oxazoles / Q000276:immunology*; D010081:Oxazolone / Q000031:analogs & derivatives / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D010080:Oxazoles; D012333:RNA, Messenger; C018361:2-phenyloxazolone; D010081:Oxazolone