[Nephrotoxicity of gentamicin in mice].
Gentamicin sulfate was tested for nephrotoxicity in B6D2F1 hybrid mice after s.c. and i.p. administration. By a series of experiments designed to study histological lesions (tubular epithelial desquamation, intracytoplasmatic coarse granulation of epithelial cells) the following procedure showed favourable results: withdrawal of drinking water 24 h before start of experiment--one daily s.c. administration during four days--post mortem examination after further 24 h. Within the epithelium of the proximal tubules two distinct lesions can be distinguished morphologically 1. an acute necrosis giving rise to acute cytolysis, 2. at a lower segment of the nephron, intracytoplasmatic storage of PAS-positive granules in cells, which are devoid of a brush border but have otherwise experienced only slight alterations. The morphology of these lesions is similar to that after administration of toxic doses of cephaloridine though the resorption of the compounds takes place in the case of gentamicin at the free cell border whereas after cephaloridine administration the compound enters the tubular epithelial cells via the basal membrane.
D000818:Animals; D005260:Female; D005839:Gentamicins / Q000633:toxicity*; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D007674:Kidney Diseases / Q000139:chemically induced* / Q000473:pathology; D007684:Kidney Tubules / Q000187:drug effects; D008297:Male; D051379:Mice; D009336:Necrosis / Q000139:chemically induced
D005839:Gentamicins
Nephrotoxizitätsstudien mit Gentamicin an der Maus.