Rapid determination of retinol (vitamin A) in serum by high pressure liquid chromatography (HPLC).
A simple, fast, and specific HPLC-method to estimate retinol (vitamin A) in serum is reported. In the presence of small amounts of acetonitrile retinol is extracted quantitatively with n-hexane from serum. Neither the use of an internal standard nor evaporation to concentrate the extract is necessary. The HPLC-method avoids interferences of substances like phytofluenes, carotinoids or retinyl palmitate which is present in serum to a fraction of about 2-5%. The HPLC-method was applied to healthy adults and healthy children. The method allows about 100 determinations per day and operator.
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D012016:Reference Values; D013997:Time Factors; D014801:Vitamin A / Q000097:blood*
D014801:Vitamin A