Cell-free translation of a T/t complex cell surface-associated gene product.
The p63/6.9 protein has previously been shown to be specified by a gene within the mouse T/t complex. All wild-type chromosomes 17 specify a basic form of p63/6.9 (p63/6.9b). It is distinguishable by two-dimensional gel electrophoresis from the acidic form of p63/6.9 (p63/6.9a), which is specified by all complete t haplotypes analyzed to date. To determine whether p63/6.9 is a direct gene product of the T/t complex, testicular cytoplasmic RNA from mice bearing the genotypes +/+, +/t and t/t was translated in a rabbit reticulocyte-derived cell-free system, and translated products were analyzed by two-dimensional gel electrophoresis. The data indicate that the difference between th allelic forms of p63/6.9 is encoded within mRNA transcribed from the T/t complex. Thus p63/6.9 is the first primary gene product identified in the T/t complex. Results obtained from both cell-free translation and pulse-chase labeling experiments indicate that the mature cell surface-associated form of p63/6.9 has not undergone detectable post-translational modification.
D000483:Alleles; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D002474:Cell-Free System; D005804:Genes, Lethal; D008297:Male; D051379:Mice; D008970:Molecular Weight; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D013091:Spermatogenesis
D000954:Antigens, Surface; D011498:Protein Precursors; D012333:RNA, Messenger