Selective expression of cell type specific cell-cell adhesion molecules in mouse hybrid cells.
Ca2+-dependent cell-cell adhesion systems (CDS) are present in a variety of cells which can be grouped into at least two qualitatively different types, the teratocarcinoma type (t-CDS) and the fibroblast type (f-CDS), where different classes of adhesion molecules operate, respectively. In order to study the regulatory mechanisms of expression of different CDS types, we made cell hybrids between teratocarcinoma OTF9 cells (t-CDS) and fibroblast L cells (f-CDS), and between OTF9 cells (t-CDS) and hepatoma MH cells (no CDS). We thus examined which type of CDS is expressed in hybrid clones using a probe, an antibody that recognizes t-CDS selectively. We isolated many hybrid clones with different phenotypes, all displaying CDS activity, and found that CDS functioning in each clone was either t-CDS or another type(s) of CDS. There were no clones in which both t-CDS and another type(s) of CDS are active. We therefore suggested that the expression or function of t-CDS and other types of CDS is mutually exclusive within a single cell.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000941:Antigens / Q000032:analysis*; D002118:Calcium / Q000494:pharmacology*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D002449:Cell Aggregation; D002460:Cell Line; D005347:Fibroblasts; D006822:Hybrid Cells; D007739:L Cells; D008114:Liver Neoplasms, Experimental; D051379:Mice; D013724:Teratoma
D000941:Antigens; D015815:Cell Adhesion Molecules; D000469:Alkaline Phosphatase; D002118:Calcium