Modification of enzymatic activity and difference spectra of cytochrome P-450 from various sources by cholesterol side chain cleavage inhibitors.
Several groups of compounds were tested for their ability to inhibit cholesterol side chain cleavage and induce spectral change in cytochrome P-450 from bovine corpus luteum, bovine adrenal cortex, and human placental mitochondria. The substances tested include: steroids, pyridines, glutarimides, anilines and imidazoles. Good correlation was found between spectral change and enzymatic inhibition, especially in the corpus luteum which has only a single P-450-linked steroid hydroxylase. The cholesterol side chain cleavage enzyme systems from each of the three sources appear to have similar affinities for the inhibitors, which adds further support to the concept that these cytochrome P-450s are functionally identical.
D000302:Adrenal Cortex / Q000201:enzymology*; D000616:Aminoglutethimide / Q000494:pharmacology; D000814:Aniline Compounds / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000037:antagonists & inhibitors*; D003338:Corpus Luteum / Q000201:enzymology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005260:Female; D005984:Glutethimide / Q000494:pharmacology; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology*; D010088:Oxidoreductases / Q000037:antagonists & inhibitors*; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D011725:Pyridines / Q000494:pharmacology; D013256:Steroids / Q000494:pharmacology
D000814:Aniline Compounds; D007093:Imidazoles; D011725:Pyridines; D013256:Steroids; D000616:Aminoglutethimide; D003577:Cytochrome P-450 Enzyme System; D005984:Glutethimide; D010088:Oxidoreductases; D002786:Cholesterol Side-Chain Cleavage Enzyme