[Detection of the clusters of negative charges on the surface of erythrocyte cytoplasmic membranes by fluorescent probes].
When charges on the membrane surface are distributed unequally and, especially, when they are in clusters, the concept of Goue-Chapman is not valid. Clusters of the negative charges on the cell surface can be detected by the combination of two fluorescent probes and a polycation which is capable of neutralizing the electrostatic field of the cluster. Clusters of the negative charges have been detected on the membranes of erythrocyte ghosts. Protamine was used as polyanion and fluorescent probes ANS and DSM were used as a fluorescent anion and cation, respectively.
D000838:Anions; D002412:Cations; D004553:Electric Conductivity; D004910:Erythrocyte Membrane / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis*; D005456:Fluorescent Dyes; D006801:Humans; D011479:Protamines
D000838:Anions; D002412:Cations; D005456:Fluorescent Dyes; D011479:Protamines
Obnaruzhenie klasterov otritsatel'nykh zariadov na poverkhnosti plazmaticheskikh membran éritrotsitov s pomoshch'iu fluorestsentnykh zondov.