title: Isolation of the homogeneous constituents from non-diffusible sugar-peptide (NSP) fraction originating from bovine plasma. III. The method of isolation and partial characterization of the four peptide constituents.
abstract: The purification of four different peptides from the NSP fraction of bovine plasma, using: organic solvents extraction, column Sephadex G-50 filtration, thin-layer Sephadex G-25 electrophoresis in 5 M guanidine hydrochloride and thin-layer Bio-Gel P-6 electrophoresis methods was described. The molecular weights of the isolated peptides and their amino acid composition were determined. The dose-dependent effect two of the four peptides on 3H-thymidine incorporation into BHK-21 and BHK/RSV cells was described. The results support previous observations that the NSP fraction contains a peptide growth prometing factor and a potent peptide growth inhibitor. The possible physiological role of the peptide growth inhibitor was discussed.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000378:metabolism; D008970:Molecular Weight; D010455:Peptides / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism
chemical_list: D000596:Amino Acids; D010455:Peptides; D013936:Thymidine