A cytochemical study of lectin receptors on isolated chick neural retina neurons in vitro.
The cell body, neurite and growth cone of isolated retinal neurons have been compared on the basis of their ability to bind a number of fluorescently labelled lectins, each possessing a unique carbohydrate specificity. The susceptibility of the respective binding patterns following pretreatment of these fixed cells with either neuraminidase or trypsin was also investigated. Neuronal cell bodies displayed the most intense binding of each lectin, with localization of limulin binding (specific for sialic acid) predominantly to the neurite hillock, the point on the cell body from which the neurite projects. Limulin binding was almost totally abolished by pretreatment with either neuraminidase or trypsin. In contrast to the cell body, limulin binding to the neurite or growth cone was not detected. These regions of the cell apparently possessed sialic acid, however, since pretreatment with neuraminidase reduced wheat germ agglutinin binding (to N-acetylglucosamine) and markedly enhanced Dolichos biflorus agglutinin binding (to N-acetylgalactosamine) to both the neurite and growth cone. The initially low binding of Dolichos biflorus agglutinin to the neurite and growth cone was slightly enhanced by pretreatment with trypsin. Uniformly low levels of binding of either Ricinus communis agglutinin 60 (galactose, N-acetylgalactosamine) or R. communis agglutinin 120 (galactose) was observed over the entire neuron. R. communis agglutinin 120 binding was not enhanced by pretreatment with neuraminidase. Receptors for either concanavalin A (mannose, glucose) or Ulex europaeus agglutinin I (fucose) were abundant over the entire nerve cell with the former exhibiting more marked trypsin sensitivity. From these data, it is apparent that the repertoire of lectin binding sites of the neurite and growth cone of these differentiating nerve cells differs markedly from that of the cell body, which itself demonstrates some degree of regionalization.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003208:Concanavalin A / Q000378:metabolism; D006241:Haptens / Q000494:pharmacology; D037102:Lectins / Q000378:metabolism; D009439:Neuraminidase / Q000494:pharmacology; D009474:Neurons / Q000378:metabolism; D037121:Plant Lectins; D011975:Receptors, Mitogen / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*; D012276:Ricin / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology; D014909:Wheat Germ Agglutinins
D006241:Haptens; D037102:Lectins; D037121:Plant Lectins; D011975:Receptors, Mitogen; D014909:Wheat Germ Agglutinins; C036554:dolichos biflorus agglutinin; C015323:limulin; D003208:Concanavalin A; D012276:Ricin; D009439:Neuraminidase; D014357:Trypsin