The solubilization of the L and M antigens from sheep red cell membranes.
The Lp, L1 and M antigens from sheep red cells were solubilized using the non-ionic detergent Triton X-100 in the presence of dithiothreitol. Recovery rates were improved when membranes were sonicated at 4 degrees C in the presence of the detergent; values in the range 16-25% (M) and 9-17% (Lp and L1) were achieved for recovery.
D000818:Animals; D003902:Detergents; D004910:Erythrocyte Membrane / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D008951:MNSs Blood-Group System / Q000302:isolation & purification*; D017830:Octoxynol; D011092:Polyethylene Glycols; D012756:Sheep; D012995:Solubility
D003902:Detergents; D008951:MNSs Blood-Group System; D011092:Polyethylene Glycols; D017830:Octoxynol