In vivo induction of granulopoiesis in visceral yolk-sac cells by foetal hepatic factors.
In order to evaluate the hypothetical activity of foetal hepatic factors on putative yolk-sac haemopoietic stem cells we used the Double Diffusion Chamber (DDC) techniqUe. The DDC were made of a regulator compartment, where foetal hepatic tissue was introduced and a test compartment where visceral yolk-sac cells were cultured. In this system a hepatic signal induced the yolk-sac stem cells to differentiate along the granulocytic pathway but did not stimulate yolk-sac CFUs growth. Contrary to CFUs originating from foetal liver or adult bone marrow, yolk-sac CFUs do not increase numerically in diffusion chamber culture.
D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004058:Diffusion; D005260:Female; D006098:Granulocytes / Q000166:cytology*; D006410:Hematopoiesis; D066298:In Vitro Techniques; D008099:Liver / Q000196:embryology*; D051379:Mice; D008809:Mice, Inbred C3H; D051381:Rats; D011919:Rats, Inbred Strains; D015017:Yolk Sac / Q000166:cytology*