Further studies on 4-hydroxy-3-methoxyphenylglycol oxidation in humans: effect of pool expansion and stereochemistry.
The in vivo oxidation of the norepinephrine metabolite 4-hydroxy-3-methoxyphenylglycol (HMPG) to 4-hydroxy-3-methoxymandelic acid was studied in man with two different doses of deuterium-labeled HMPG and a tracer dose of [14C]HMPG. HMPG oxidation appeared to be dose-dependent with an oxidation of 62-70% for doses below or equal to 2.2 mumol. With the use of a capillary column coated with an optically active phase (Chirasil-Val) and gas chromatography mass-spectrometry the human urinary excretions of the two stereoisomers of deuterium-labelled HMPG (free + conjugates) were found to be equal.
D002250:Carbon Radioisotopes; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D006018:Glycols / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008734:Methoxyhydroxyphenylglycol / Q000378:metabolism* / Q000652:urine; D010084:Oxidation-Reduction
D002250:Carbon Radioisotopes; D006018:Glycols; D008734:Methoxyhydroxyphenylglycol; D003903:Deuterium