title: Recovery of Chlamydia trachomatis from patients of southeastern venereal disease clinic.
abstract: The authors determined the incidences of Chlamydia trachomatis colonization obtained by use of two methods of isolation in 169 specimens from individuals attending a venereal disease clinic. Speciments were collected in phosphate sucrose buffer and planted simultaneously onto McCoy cells previously treated with 5-iodo-2-deoxyuridine (IUDR) and with cycloheximide. As controls, specimens were obtained from 76 hospital employees or medical students without clinical signs of infection and cultured in a similar manner. Qualitatively, recoveries of Chlamydia trachomatis inclusions from the differently treated cells were the same, but the number of inclusions per ml was greater for the cycloheximide-treated cells. Recoveries of Chlamydia trachomatis from the venereal disease clinic population were 33% for those patients who also had simultaneous culture positive for Neisseria gonorrhoeae and 42% for those with nongonococcal urethritis. The recovery of Chlamydia trachomatis from the control group was 5%.
mesh_terms: D002478:Cells, Cultured / Q000187:drug effects; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000453:epidemiology; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D006801:Humans; D007065:Idoxuridine / Q000494:pharmacology; D008297:Male; D010044:Outpatient Clinics, Hospital; D012749:Sexually Transmitted Diseases / Q000175:diagnosis*; D013048:Specimen Handling / Q000379:methods; D014526:Urethritis / Q000209:etiology
chemical_list: D003470:Culture Media; D003513:Cycloheximide; D007065:Idoxuridine