Influence of protein biosynthesis inhibitors on stimulated renal p-aminohippurate excretion in rats.
The renal excretion of p-aminohippurate (PAH) can be stimulated by repeated administration of cyclopenthiazide (5 mg/100 g b.w., i.p. for 3 days, twice daily). The reason for this seems to be an increased renal tubular transport capacity for weak organic acids. Inhibitors of protein biosynthesis as azauracil, actinomycin D, neomycin and cycloheximide influence the cyclopenthiazide stimulated p-aminohippurate excretion. Azauracil (5 mg/100 g b.w.), an inhibitor of replication is effective in the same way as inhibitors acting on transcription and translation (actinomycin D 7.5-60 micrograms/100 g b.w.; neomycin 5-10 mg) 100 g b.w.; cycloheximide 0.02-0.05 mg/100 g b.w., i.p. for 4 days, once daily). Probably these substances inhibit the de novo synthesis of carrier proteins, increased by stimulation of renal tubular transport processes.
D000618:Aminohippuric Acids / Q000652:urine*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002701:Chloramphenicol / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003518:Cyclopenthiazide / Q000494:pharmacology*; D003609:Dactinomycin / Q000494:pharmacology; D004232:Diuretics; D005260:Female; D007668:Kidney / Q000378:metabolism*; D009355:Neomycin / Q000494:pharmacology; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D049993:Sodium Chloride Symporter Inhibitors / Q000494:pharmacology*; D014498:Uracil / Q000031:analogs & derivatives / Q000494:pharmacology; D010130:p-Aminohippuric Acid / Q000652:urine*
D000618:Aminohippuric Acids; D004232:Diuretics; D049993:Sodium Chloride Symporter Inhibitors; D003609:Dactinomycin; D014498:Uracil; D002701:Chloramphenicol; D003513:Cycloheximide; C100248:azauracil; D009355:Neomycin; D003518:Cyclopenthiazide; D010130:p-Aminohippuric Acid