Isolation and characterization of a Chlamydomonas L-asparaginase.
An L-asparaginase (EC 3.5.1.1) specific for L-asparagine has been purified from a marine Chlamydomonas species, the first such enzyme to be purified from a microalga. The purified enzyme (mol.wt. 275 000) possessed a Km for asparagine of 1.34 x 10(-4) M and showed limited antitumour activity in an antilymphoma assay in vivo. Properties of the enzyme are contrasted with those of asparaginases from prokaryotic and eukaryotic sources.
D000328:Adult; D000818:Animals; D001215:Asparaginase / Q000302:isolation & purification* / Q000378:metabolism / Q000627:therapeutic use; D001216:Asparagine / Q000378:metabolism; D002460:Cell Line; D002696:Chlamydomonas / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000188:drug therapy; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H
D001216:Asparagine; D001215:Asparaginase