A method of estimating radial distribution function of protein particles in membranes from freeze-fracture electron micrographs.
A computational method for estimating the radial distribution function of particles seen in electron micrographs of freeze-fractured membranes is presented. The method overcomes the previous difficulty of measurements of this quantity from freeze-fracture electron micrographs--the systematic error due to the finite size of the sample areas in the freeze-fracture. By multiplying the measurements by weights specific to the shape of the sample area, the method lessens the errors due to the boundaries of the area and allows a synthesis of the average radial distribution function from counts over many small separate areas.
D003201:Computers; D005614:Freeze Fracturing; D008565:Membrane Proteins / Q000378:metabolism*; D008566:Membranes / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D012984:Software
D008565:Membrane Proteins