title: Evidence for the spontaneous formation of interspecies hybrid molecules of human, rat and bovine serum albumins.
abstract: Utilizing electrophoretic and gel filtration techniques it was shown that a bovine C-terminal peptic fragment [residues 307-582] spontaneously forms interspecies hybrid molecules with three complementary N-terminal fragments derived from human [residues 1-308; 49-308] and rat [residues 1-308] albumins. The fragments associate with 1:1 stoichiometry to produce an albumin-like complex which has a molecular weight and electrophoretic mobility similar to intact albumin. These data demonstrate, for the first time, that albumin fragments derived from different species associate in a complementary fashion and provide direct evidence that the tertiary structure may be highly conserved.
mesh_terms: D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis; D055503:Protein Multimerization; D051381:Rats; D012709:Serum Albumin / Q000302:isolation & purification / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D010446:Peptide Fragments; D012709:Serum Albumin; D012710:Serum Albumin, Bovine