Phalloidin-induced morphological and functional changes of rat liver.
The hepatotoxic effect of phalloidin, a mushroom poison, was examined in whole liver and in isolated liver cell plasma membranes. By electron microscopy, a thickening of the pericanalicular microfilamentous network was noted. Bile canalicular enriched plasma membrane fractions of livers from phalloidin-treated animals contained increased amounts of microfilaments. Analysis of the peptides in these fractions, by sodium dodecyl sulfate polyacrylamide gel electrophoresis, indicated that although the actin peptide bands were prominent, a band apparently corresponding to myosin was drastically reduced. The morphological change was accompanied by a reduced bile flow and biliary excretion of bile acids, proteins and the exogenous organic anion dibromosulphthalein. These changes may result from a modification of the microfilaments, suggesting a role of the microfilaments in the liver membrane structure and function.
D000818:Animals; D001646:Bile / Q000378:metabolism; D001648:Bile Canaliculi / Q000187:drug effects; D002462:Cell Membrane / Q000032:analysis; D003599:Cytoskeleton / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D007365:Intercellular Junctions / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D009842:Oligopeptides / Q000633:toxicity*; D010455:Peptides / Q000032:analysis; D010590:Phalloidine / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains
D009842:Oligopeptides; D010455:Peptides; D010590:Phalloidine