Prevention of leakage of Di-(2-ethylhexyl)phthalate from blood bags by glow discharge treatment and its effect on aggregability of stored platelets.
Platelets storage in glow discharge treated PVC bags was studied. The amount of leaked di-(2-ethylhexyl) phthalate (DEHP) was 150-200 micrograms/ml/day in the nontreated PVC bags, but only 20-40 micrograms/ml in the treated bags after 48 h. The adhesion of silicone to PVC was much improved, and consequently, uniform coating with silicone became feasible. The decrease of the ability of platelets to aggregate was accelerated by DEHP. When stored platelets were resuspended in fresh plasma, the ability to aggregate was gradually restored. However, the degree of restoration of the ability of platelets which had been incubated with DEHP was low. When platelets were stored in the glow discharge treated and then silicone-coated PVC bags, their adhesion on the surface and the decrease of their function were prevented.
D001793:Blood Preservation; D004051:Diethylhexyl Phthalate / Q000009:adverse effects / Q000032:analysis / Q000494:pharmacology*; D004349:Drug Packaging; D006801:Humans; D010795:Phthalic Acids / Q000494:pharmacology*; D010973:Platelet Adhesiveness / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects*; D011143:Polyvinyl Chloride / Q000494:pharmacology; D013499:Surface Properties; D013997:Time Factors
D010795:Phthalic Acids; D011143:Polyvinyl Chloride; D004051:Diethylhexyl Phthalate