title: Factor XIIIa-catalyzed cross-linking of platelet and muscle actin. Regulation by nucleotides.
abstract: Actin from human blood platelets or rabbit skeletal muscle can serve as substrate for factor XIIIa. The latter catalyzes the incorporation of 1.5-2 mol monodansylcadaverine/mol rabbit actin and 0.5 mol/mol platelet actin. Highly cross-linked platelet and muscle actin polymers form in the absence of added amines, indicating the presence of both acceptor and donor sites. As expected, the cross-link was found to be a gamma-glutamyl-epsilon-lysine bone, with an average of 0.3-0.4 mol dipeptide/mol platelet actin. Both cross-linking and amine incorporation are prevented by ATP, ADP, GTP, CTP, but not by AMP and cyclic AMP. These nucleotides may have important regulatory role in muscle and non-muscle systems.
mesh_terms: D000199:Actins / Q000097:blood / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002103:Cadaverine / Q000031:analogs & derivatives / Q000378:metabolism; D003570:Cytidine Triphosphate / Q000494:pharmacology; D003619:Dansyl Compounds / Q000378:metabolism; D005176:Factor XIII / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006801:Humans; D046911:Macromolecular Substances; D009132:Muscles / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis; D011817:Rabbits
chemical_list: D000199:Actins; D003619:Dansyl Compounds; D046911:Macromolecular Substances; D010446:Peptide Fragments; D000244:Adenosine Diphosphate; D003570:Cytidine Triphosphate; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D005176:Factor XIII; C008542:monodansylcadaverine; D002103:Cadaverine