title: A monoclonal antibody against catalytic subunits of acetylcholinesterase in the electric organ of an electric ray, Narke japonica.
abstract: A hybridoma cell line secreting a monoclonal antibody against catalytic subunits of acetylcholinesterase in the electric organ of Narke japonica has been obtained by using a mouse immunized with the nerve terminal membranes prepared from the electric organ of Narke japonica. This monoclonal antibody (Nj-501) reacted with asymmetric, low salt-soluble and detergent-soluble forms of acetylcholinesterase of the electric organ of Narke japonica. Immunoblot analysis showed that the catalytic subunits of the asymmetric, low salt-soluble and detergent-soluble forms of acetylcholinesterase were polypeptides of molecular weight (Mr) 70K, 70K / 66K and 66K, respectively. Nj-501 markedly inhibited the activity of all these forms of acetylcholinesterase.
mesh_terms: D000110:Acetylcholinesterase / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001665:Binding Sites; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D004557:Electric Organ / Q000201:enzymology* / Q000294:innervation; D005260:Female; D005399:Fishes / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D009411:Nerve Endings / Q000201:enzymology
chemical_list: D000911:Antibodies, Monoclonal; D000110:Acetylcholinesterase