Molecular mechanisms of odor sensing. VI. Some biochemical characteristics of a possible receptor for amino acids from the olfactory epithelium of the skate Dasyatis pastinaca and carp Cyprinus carpio.
The 25 000 x g pellet of skate and carp olfactory tissue was found to contain structures with high affinity for amino acids (Kd approximately 10(-10) M). Results from competition experiments suggest that a multiplicity of types of olfactory binding sites exists in the skate and carp. Some parameters of a possible receptor for amino acids were evaluated. The isoelectric point of these sites solubilized with Triton X-100 was 5.35 and the molecular weight was about 120 000-150 000.
D000596:Amino Acids / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D002347:Carps / Q000502:physiology; D004848:Epithelium / Q000502:physiology; D005399:Fishes / Q000502:physiology*; D007700:Kinetics; D008465:Mechanoreceptors / Q000502:physiology*; D009812:Odorants; D009831:Olfactory Mucosa / Q000502:physiology*; D012903:Smell
D000596:Amino Acids