Radioimmunoassay of prostaglandins in the semen of fertile men.
Specific antisera and iodinated tracers, as well as strict conditions of sample storage PG extraction and purification, were used to estimate prostaglandins (microgram/ml) in the semen of fertile men: 19-OH-PGE (1 + 2) (350), 19-OH-PGF-alpha (22), PGE-2 (15), PGE-1 (6.2), PGF-2 alpha (2.3), PGF-1 alpha (1.3), 6-keto-PGF-1 alpha (less than 1), PGD-2, 13,14-dihydro-15-keto-PGF alpha and TXB-2 (less than 0.1). Only PGE-2 (50 pg/ml) was detectable in rat seminal fluid.
D000527:Alprostadil / Q000031:analogs & derivatives*; D000818:Animals; D015237:Dinoprost / Q000031:analogs & derivatives*; D005298:Fertility; D006801:Humans; D008297:Male; D011458:Prostaglandins E / Q000032:analysis*; D011460:Prostaglandins F / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats; D012661:Semen / Q000032:analysis*
D011458:Prostaglandins E; D011460:Prostaglandins F; C025128:19-hydroxyprostaglandin E1; C025183:19-hydroxyprostaglandin F; D015237:Dinoprost; D000527:Alprostadil