The structure of S1-decorated actin filaments calculated from x-ray diffraction data with phases derived from electron micrographs.
A composite data set in Fourier space has been produced from electron micrographs of negatively stained decorated actin and X-ray fibre diffraction patterns of glycerinated insect muscle "decorated" with rabbit myosin cross-bridges. It is shown that a simple model of actin and myosin may be adjusted to fit this data. The model conforms to the Taylor-Amos geometry for the acto-myosin interaction in accordance with the idea that the cross-bridge binds to two neighbouring actin monomers on different strands.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D003599:Cytoskeleton / Q000648:ultrastructure*; D006430:Hemiptera; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008958:Models, Molecular; D009132:Muscles / Q000648:ultrastructure; D009218:Myosins / Q000378:metabolism; D011487:Protein Conformation; D014961:X-Ray Diffraction / Q000379:methods
D000199:Actins; D046911:Macromolecular Substances; D009218:Myosins