Re-evaluation of glucagon1-6: the N-terminal hexapeptide of glucagon is not biologically active in the hepatic adenylate cyclase system.
The N-terminal hexapeptide of glucagon and the corresponding carboxamide analog, were prepared by solid-phase synthesis and tested for biological activity in the hepatic adenylate cyclase system. Both peptides were found to be inactive, even at concentrations of 10 mM. The differences observed in the activity of our compounds compared to previous reports, is ascribed to the presence of a contaminant found in earlier preparations which activates adenylate cyclase.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D055598:Chemical Phenomena; D002621:Chemistry; D004340:Drug Contamination; D004789:Enzyme Activation / Q000187:drug effects; D005934:Glucagon / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D013329:Structure-Activity Relationship
D010446:Peptide Fragments; C036296:glucagon (1-6); D005934:Glucagon; D000262:Adenylyl Cyclases