Analysis of the DNA-terminal protein from different serotypes of human adenovirus.
The DNA-terminal proteins from adenovirus type 12, type 3, and type 5 were analyzed after labeling in vitro with 125I by the chloramine T method, and were shown to be serotype specific. We also studied the kinetics of cleavage by alkali of the terminal protein-DNA linkage and showed that the half-time of cleavage with either 0.1 M NaOH or 0.5 M piperidine at 37 degree C is about 15 to 30 min. The substitution of the volatile base piperidine for NaOH in this procedure provided a useful tool for rapid analysis of the labeled protein.
D000260:Adenoviruses, Human / Q000032:analysis* / Q000276:immunology; D002352:Carrier Proteins / Q000032:analysis*; D004247:DNA / Q000032:analysis*; D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D012703:Serotyping; D014764:Viral Proteins / Q000032:analysis*
D002352:Carrier Proteins; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D010446:Peptide Fragments; D014764:Viral Proteins; D004247:DNA