Separation of human lymphocytes on Ficoll-Paque gradients: stimulation of cells and depletion of a concanavalin-A responsive radioresistant subpopulation.
A subpopulation of human lymphocytes separated on Ficoll-Paque gradients showed an ultrastructural phenotype characteristic of stimulated cells. Thymidine incorporation was increased fivefold compared with unseparated (Buffy coat) controls. The Ficoll-Paque separated lymphocytes were more sensitive to gamma radiation than unseparated lymphocytes and showed a decreased capability to undergo transformation in response to concanavalin A. Transformation in response to phytohemagglutinin and pokeweed mitogen was the same as for unseparated control lymphocytes. These results are interpreted as the selective depletion of a Con A-responsive T-cell fraction by Ficoll-Paque separation.
D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D003208:Concanavalin A / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects* / Q000648:ultrastructure; D008854:Microscopy, Electron
D003208:Concanavalin A