[Ultrastructure of L-line lipoblast-like cells during cloning in a medium with a high serum concentration].
The ultrastructure of lipoblast-like cells produced by clones of transformed culture of strain in the medium with an increased (60%) concentration of bovine serum was studied. The aim of using the stimulator rich in adipogenous factors was the elucidation of the origin of lipid accumulation in some of cell types during heterogeneous differentiation of clones. In these conditions non-differentiated lipoblasts assumed the fine structure of mature lipid cells. The statement of the marker significance of lipid accumulation in non-differentiated cells of the clones and the conversion of these cells under the influence of non-specific stimulator to the mature lipocytes confirms the data on conservation of over-all cytogenetic potentials and the capacity of their realization in the cells of transformed cultures. It is suggested that the increased concentration of bovine serum in the cultural medium not only stimulates differentiation (by any way determined cells), but is also capable of acting on the stem elements produced by the clone. In that case the influence of the stimulator on the trends of heterogeneous differentiation is not unlikely.
D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D002999:Clone Cells / Q000187:drug effects / Q000648:ultrastructure; D003238:Connective Tissue / Q000187:drug effects / Q000648:ultrastructure*; D003470:Culture Media / Q000494:pharmacology*; D007106:Immune Sera / Q000494:pharmacology*; D007739:L Cells / Q000187:drug effects / Q000648:ultrastructure*; D051379:Mice; D008854:Microscopy, Electron
D003470:Culture Media; D007106:Immune Sera
Ul'trastruktura lipoblastopodobnykh kletok linii L v usloviiakh klonirovaniia v srede s vysokoĭ kontsentratsieĭ syvorotki.