Interaction of supraspinal, serotonergic, and opiate systems during hemorrhage.
Anesthetized cats were subjected to hemorrhage to a mean arterial blood pressure of 40 +/- 1 mmHg. After 60 min of oligemia pressure regulation was stopped, and naloxone hydrochloride or an equivalent volume of 0.9% saline was given intravenously as a bolus (2 mg x kg-1) followed by a continuous infusion (2 mg x kg-1 x hr-1). In other cats supraspinal systems were ablated by functional decapitation (FD) or serotonergic systems were ablated by IP injection of parachlorophenylalanine (pCPA, 300 mg/kg, 48 h prior to hemorrhage). Cats subjected to ablation of supraspinal or serotonergic neurons showed a distinct pressor response after naloxone. Although cats with intact neuronal systems failed to show a distinct pressor response to naloxone, the arterial pressures of these cats were significantly greater than hemorrhaged cats given saline (P less than 0.05, unpaired t). Our results suggest that a supraspinal system and a serotonergic system can interact to limit the pressor response to naloxone in hemorrhagic shock.
D000320:Adrenergic Fibers / Q000503:physiopathology; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001933:Brain Stem / Q000503:physiopathology*; D002415:Cats; D004723:Endorphins / Q000502:physiology*; D005260:Female; D006339:Heart Rate / Q000187:drug effects; D008297:Male; D009270:Naloxone / Q000008:administration & dosage; D009292:Narcotic Antagonists / Q000008:administration & dosage; D011903:Raphe Nuclei / Q000503:physiopathology*; D012771:Shock, Hemorrhagic / Q000503:physiopathology*; D012965:Sodium Chloride / Q000008:administration & dosage; D013116:Spinal Cord / Q000503:physiopathology*; D013119:Spinal Cord Injuries / Q000503:physiopathology
D004723:Endorphins; D009292:Narcotic Antagonists; D009270:Naloxone; D012965:Sodium Chloride