Comparison between dissociation and inhibition of association of DNA/anti-DNA complexes.
A study was made of the increases in ionic strength (mu) and in pH that would effect: a) the inhibition of association of anti-double stranded (ds) DNA and dsDNA, and b) the dissociation of anti-dsDNA from dsDNA, with respect to high avidity as well as to low avidity human anti-dsDNA antibodies, using Crithidia luciliae kinetoplasts as a source of dsDNA. The results obtained appear to indicate that the electrostatic component of the antigen-antibody bond does not become stronger with time (for times spans less than 1 hr).
D000433:1-Propanol / Q000494:pharmacology; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D002118:Calcium / Q000494:pharmacology; D004247:DNA / Q000276:immunology*; D004277:DNA, Single-Stranded / Q000276:immunology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D009690:Nucleic Acid Conformation; D009994:Osmolar Concentration; D011485:Protein Binding / Q000187:drug effects
D000936:Antigen-Antibody Complex; D004277:DNA, Single-Stranded; D004247:DNA; D000433:1-Propanol; D002118:Calcium