Effect of retinol and retinoic acid on permeability, electrical resistance and phase transition of lipid bilayers.
Retinol and retinoic acid have been incorporated into the artificial membrane systems, planar bimolecular lipid membranes and liposomes, and their effects on several membrane parameters have been measured. 1. Retinol and retinoic acid increased the permeability of egg lecithin liposomes to K+, I- and glucose when incorporated into the membranes at levels as low as 0.5 membrane mol%. Retinoic acid influenced permeability more than did retinol for each of the solutes tested. 2. Retinol and retinoic acid both decreased the electrical resistance of egg lecithin-planar bimolecular lipid membranes from 0.5 to 8 membrane mol%. Retinoic acid effected a larger change than did retinol. 3. Retinol and retinoic acid increased the permeability of dimyristoylphosphatidylcholine and dipalmitoylphosphatidylcholine liposomes to water at 1.0 and 3.0 membrane mol%. A larger effect on water permeability was measured for retinoic acid than for retinol. 4. Retinol and retinoic acid at 1.0 and 3.0 membrane mol% were shown to lower the phase-transition temperature of liposomes composed of dimyristoylphosphatidylcholine or dipalmitoylphosphatidylcholine. Phase-transition temperatures were monitored by abrupt changes in water permeability and liposome size associated with the transition. Retinoic acid lowered the phase-transition temperature of dimyristoylphosphatidylcholine liposomes more than did retinol, while both retinoids had almost the same effect on dipalmitoylphosphatidylcholine liposomes.
D004134:Dimyristoylphosphatidylcholine; D005947:Glucose; D007454:Iodides; D007700:Kinetics; D008051:Lipid Bilayers; D008081:Liposomes; D010539:Permeability; D010713:Phosphatidylcholines; D011188:Potassium; D011663:Pulmonary Surfactants; D013329:Structure-Activity Relationship; D014212:Tretinoin; D014801:Vitamin A
D007454:Iodides; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D011663:Pulmonary Surfactants; D014801:Vitamin A; D014212:Tretinoin; D005947:Glucose; D011188:Potassium; D004134:Dimyristoylphosphatidylcholine