Aminoglycoside-modifying enzymes associated with hospital isolates of Gram-negative rods.
Multi-resistant Enterobacteriaceae and Pseudomonas isolated from clinical specimens at RNS were characterized for resistance phenotype and transferred resistant phenotype. These isolates were obtained over a two year period from different specimens and from patients with varying clinical syndromes. Twenty-six of these isolates were further characterized with respect to their aminoglycoside modifying enzymes. Only three enzymes were detected: AAC(3)-I, APH(3')-I and AAD(2")-II. The substrate range of these enzymes was investigated and not always found to coincide with the transferred phenotype. AAD(2")-II was found only in association with APH(3')-I and not alone. The substrate range of AAC(3)-I from Pseudomonas was different from AAC(3)-I from Enterobacteriaceae but not sufficiently dissimilar to classify them as separate isoenzymes. The relatively few aminoglycoside-inactivating enzymes suggest that within this hospital there may be a limited gene pool for these enzymes.
D000617:Aminoglycosides / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000201:enzymology*; D004352:Drug Resistance, Microbial; D004755:Enterobacteriaceae / Q000201:enzymology; D010641:Phenotype; D011815:R Factors
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents