Analysis and quantitation of a metabolite of doxycycline in mice, rats, and humans by high-performance liquid chromatography.
A metabolite of doxycycline has not previously been isolated. In this paper it is demonstrated that doxycycline is metabolized in mice, rats, and humans. By means of high-performance liquid chromatography and consecutive gel chromatography one metabolite of doxycycline was isolated from animal organs and human urine. The metabolite was tentatively identified as N-monodemethyldoxycycline by mass spectral and spectrophotometric analyses. The rate of metabolism could be enhanced by pretreatment of the animals with phenobarbital, an inducing agent of the drug-metabolizing enzymes.
D000328:Adult; D000818:Animals; D001711:Biotransformation; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004318:Doxycycline / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D006801:Humans; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D051381:Rats; D011919:Rats, Inbred Strains
D004318:Doxycycline