Direct measurement of in vitro antibody production using an enzyme-linked immunosorbent assay.
A rapid and sensitive enzyme-linked immunoassay is described for quantitation of the secretion of cellular products during short-term (1-20 h) culture in vitro. The method is based on culture of cells, serially diluted in antigen or antibody-coated polystyrene microtitre wells. The wide applicability of this method for measurement of anti-hapten antibody, immunoglobulin and lymphokine production is stressed.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000917:Antibody Formation; D000936:Antigen-Antibody Complex; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs / Q000276:immunology; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008214:Lymphocytes / Q000276:immunology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D007075:Immunoglobulin M