title: Cathodally migrating creatine kinase in sera of patients treated with theophylline, and its diagnostic implications.
abstract: Sera from 30 patients, selected only on the basis of having serum theophylline concentrations greater than or equal to 18 mg/L, were assayed for total creatine kinase (CK), isoenzyme CK-MB, and cathodally migrating CK (mCK-2). CK-MB was measured by ion-exchange chromatography. mCK-2 was detected by electrophoresis on agarose gel, with fluorometric scanning. The mCK-2 variant was found in 37% of these 30 patients. None of the patients in the mCK-2 group had CK-MB values exceeding the cutoff value associated with myocardial infarction, so extensive cardiac ischemia was considered unlikely. A review of the patients' records supported this conclusion, and no particular association with any specific disease was found among the mCK-2 group. Investigations with use of goat anti-CK-MM and anti-theophylline indicated that neither CK-MM nor theophylline were present in the mCK-2. Results of specific radioimmunoassay for CK-B subunit were negative. The mobility of the mCK-2 peak was unresponsive to 2-mercaptoethanol, suggesting that CK of mitochondrial origin was not present. These results provide insight into the clinical relevance of mCK-2 and some information concerning its composition.
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001249:Asthma / Q000097:blood* / Q000188:drug therapy; D002648:Child; D002675:Child, Preschool; D002852:Chromatography, Ion Exchange; D003402:Creatine Kinase / Q000097:blood / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007223:Infant; D007527:Isoenzymes; D008875:Middle Aged; D013806:Theophylline / Q000097:blood* / Q000627:therapeutic use
chemical_list: D007527:Isoenzymes; D013806:Theophylline; D003402:Creatine Kinase