Purification from starfish eggs of a protein that depolymerizes actin.
A protein which is capable of depolymerizing F-actin was purified from an extract of unfertilized starfish eggs by the use of DEAE-cellulose column chromatography and hydroxylapatite column chromatography. This protein has an apparent molecular weight of 17,000. It inhibited the extent of actin polymerization as well as depolymerizing F-actin rapidly. It was shown that this protein reacts with actin at a molar ratio of 1 : 1. The properties of this protein were compared with those of profilin from mammalian tissues.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004527:Egg Proteins / Q000302:isolation & purification* / Q000378:metabolism; D005260:Female; D008970:Molecular Weight; D010063:Ovum / Q000032:analysis*; D013215:Starfish
D000199:Actins; D004527:Egg Proteins