Formation of imine derivatives between tris(hydroxymethyl)-aminomethane and saturated 3-oxo steroids during conventional work up of biological samples dissolved in Tris-HCl buffers.
Extraction with organic solvents of Tris-HCl buffer solutions containing microgram amounts of saturated 3-oxo-steroids, followed by evaporation of the organic solvent, produce a condensation product identified as the Tris-3-imino-derivative of the steroid. The yields range between 20-40%. The conditions governing the formation of the derivatives have been explored.
D055598:Chemical Phenomena; D002621:Chemistry; D002776:Cholestanes; D002778:Cholestanones; D013196:Dihydrotestosterone; D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D007097:Imines; D009994:Osmolar Concentration; D012997:Solvents; D013329:Structure-Activity Relationship; D014325:Tromethamine
D002776:Cholestanes; D002778:Cholestanones; D007097:Imines; D012997:Solvents; D014325:Tromethamine; D013196:Dihydrotestosterone