Simultaneous liquid-chromatographic determination of hypoxanthine, xanthine, urate, and creatinine in cerebrospinal fluid, with direct injection.
In this method for simultaneously determining hypoxanthine, xanthine, urate, and creatinine in cerebrospinal fluid, centrifuged sample is directly injected on a reversed-phase liquid-chromatographic column. On elution with potassium phosphate buffer the compounds are quantified by their absorbance at 260 nm. Random error (CV) was between 1.2 and 3.4% and analytical recoveries were 99-104% at physiological concentrations.
D002851:Chromatography, High Pressure Liquid; D003404:Creatinine / Q000134:cerebrospinal fluid*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007042:Hypoxanthines / Q000134:cerebrospinal fluid*; D013056:Spectrophotometry, Ultraviolet; D014527:Uric Acid / Q000134:cerebrospinal fluid*; D014970:Xanthines / Q000134:cerebrospinal fluid*
D007042:Hypoxanthines; D014970:Xanthines; D014527:Uric Acid; D003404:Creatinine