The effects of dimethylnitrosamine and allyl alcohol on primary maintenance cultures of adult rabbit hepatocytes.
Cultures of adult rabbit hepatocytes have been used to study the early toxic effects of 2 model hepatotoxins, dimethylnitrosamine and allyl alcohol. Leakage of glutamate oxaloacetate transaminase and glutamate pyruvate transaminase into the cell culture medium was a sensitive indicator of plasma membrane damage by these compounds and a dose-response relationship was observed. By contrast, gamma-glutamyltranspeptidase and alkaline phosphatase were insensitive markers. The effects of dimethylnitrosamine were slower to develop. Dimethylnitrosamine also produced a dose-related inhibition of protein synthesis after 4 h, a decrease in NADPH diaphorase and an increase in non-specific esterase after 20 h. Dimethylnitrosamine, unlike allyl alcohol, caused extensive disruption of ribosome association with the endoplasmic reticulum.
D000433:1-Propanol / Q000009:adverse effects; D000818:Animals; D002478:Cells, Cultured; D008058:Dihydrolipoamide Dehydrogenase / Q000032:analysis; D004128:Dimethylnitrosamine / Q000009:adverse effects*; D004950:Esterases / Q000032:analysis; D006651:Histocytochemistry; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D020005:Propanols; D014176:Protein Biosynthesis; D011817:Rabbits
D020005:Propanols; C006463:allyl alcohol; D000433:1-Propanol; D008058:Dihydrolipoamide Dehydrogenase; D004950:Esterases; D004128:Dimethylnitrosamine