Rapid isolation of apolipoprotein E from human plasma very low density lipoproteins by molecular sieve high performance liquid chromatography.
A rapid (less than 1 hour) and sensitive technique was developed for the isolation of apolipoprotein E from the other main components of human plasma very low density lipoproteins using molecular sieve high performance liquid chromatography with an approximate 80% recovery. The properties of the pure apoprotein were those reported in the literature for products isolated by conventional chromatographic and/or electrophoretic procedures.
D001053:Apolipoproteins / Q000097:blood* / Q000302:isolation & purification; D001057:Apolipoproteins E; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008079:Lipoproteins, VLDL / Q000097:blood* / Q000302:isolation & purification
D001053:Apolipoproteins; D001057:Apolipoproteins E; D008079:Lipoproteins, VLDL