A cell line (HBL-100) established from human breast milk.
A continuous cell line (HBL-100) was obtained from primary cultures of cells derived from an early lactation sample of human milk. There was no evidence of a breast lesion in the milk donor. Karyotype analysis showed that all metaphases contained human chromosomes including a large acrocentric marker chromosome. Both desmosomes and cytoplasmic tonofibrils were observed during early passage. HBL-100 cells exhibited several characteristics of transformation including the ability to form colonies in soft agar, an aneuploid chromosome complement, and continuous growth.
D000328:Adult; D001940:Breast; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002999:Clone Cells; D003599:Cytoskeleton / Q000648:ultrastructure; D003896:Desmosomes / Q000648:ultrastructure; D004848:Epithelium; D005260:Female; D006056:Golgi Apparatus / Q000648:ultrastructure; D006801:Humans; D007621:Karyotyping; D007774:Lactation; D008895:Milk, Human / Q000166:cytology*; D011247:Pregnancy