title: Simultaneous liquid-chromatographic determination of hypoxanthine, xanthine, urate, and creatinine in cerebrospinal fluid, with direct injection.
abstract: In this method for simultaneously determining hypoxanthine, xanthine, urate, and creatinine in cerebrospinal fluid, centrifuged sample is directly injected on a reversed-phase liquid-chromatographic column. On elution with potassium phosphate buffer the compounds are quantified by their absorbance at 260 nm. Random error (CV) was between 1.2 and 3.4% and analytical recoveries were 99-104% at physiological concentrations.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003404:Creatinine / Q000134:cerebrospinal fluid*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007042:Hypoxanthines / Q000134:cerebrospinal fluid*; D013056:Spectrophotometry, Ultraviolet; D014527:Uric Acid / Q000134:cerebrospinal fluid*; D014970:Xanthines / Q000134:cerebrospinal fluid*
chemical_list: D007042:Hypoxanthines; D014970:Xanthines; D014527:Uric Acid; D003404:Creatinine