title: Oxygen and serum complement in phagocytosis and killing of Propionibacterium acnes.
abstract: Phagocytosis and killing of Propionibacterium acnes by human peripheral blood leukocytes were promoted by normal human serum and by serum depleted of immunoglobulin G and M. Serum that had been heat-inactivated, depleted of complement component C3, or depleted of C4 and factor B, did not promote phagocytosis or killing. Depletion of complement-component C4 or factor B from normal human serum did not impair its capacity to promote phagocytosis and killing of P. acnes. These results indicate that phagocytosis of P. acnes is mediated by complement component C3, activated either by the classical or by the alternative pathway. P. acnes was phagocytosed as efficiently under anaerobic as under aerobic conditions. The bacteria were, however, killed at a considerably slower rate by anaerobically incubated leukocytes than by leukocytes incubated in air. Thus, oxygen dependent mechanisms were essential for killing of P. acnes.
mesh_terms: D000328:Adult; D000332:Aerobiosis; D000361:Agammaglobulinemia / Q000276:immunology; D001424:Bacterial Infections / Q000276:immunology*; D001790:Blood Physiological Phenomena; D003170:Complement Pathway, Alternative; D003171:Complement Pathway, Classical; D003165:Complement System Proteins / Q000502:physiology*; D006358:Hot Temperature; D006801:Humans; D007962:Leukocytes / Q000276:immunology; D010101:Oxygen Consumption; D010587:Phagocytosis; D011425:Propionibacterium acnes / Q000276:immunology
chemical_list: D003165:Complement System Proteins