Kirromycin-resistant elongation factor Tu from wild-type of Lactobacillus brevis.
Properties of the elongation factor Tu from Lactobacillus brevis which is naturally insensitive to kirromycin are described. The protein is characterized by an unusual nucleotide-binding site with increased affinity for GTP and extreme heat stability. EF-Tu is sensitive to pulvomycin in the assay of polyphenylalanine synthesis. However, the failure of the protein to display pulvomycin-dependent GDP-binding and GTPase activity indicates that pulvomycin action in L. brevis differs from that in E. coli.
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004352:Drug Resistance, Microbial; D006027:Glycosides / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000378:metabolism; D006358:Hot Temperature; D007778:Lactobacillus / Q000187:drug effects*; D010444:Peptide Elongation Factor Tu; D010445:Peptide Elongation Factors / Q000378:metabolism*; D011728:Pyridones / Q000494:pharmacology
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D006027:Glycosides; D010445:Peptide Elongation Factors; D011728:Pyridones; C012521:pulvomycin; D006160:Guanosine Triphosphate; D010444:Peptide Elongation Factor Tu; C008536:mocimycin