Interaction of human polymorphonuclear leukocyte (PMN) elastase with human IgM. Production of a factor enhancing PMN migration in vitro.
Digestion in vitro of human monoclonal IgM by human polymorphonuclear leukocyte (PMN) elastase produces small peptides in addition to larger fragments. These small peptides were shown to enhance PMN locomotion in vitro, as tested by the migration-under-agarose assay. The small peptides from elastase degradation of the Fc5u portion of the IgM molecule also increase PMN migration in vitro. The migration-enhancing factor is stable at 60 degrees C for 20 minutes but is inactivated by boiling for 10 minutes, and has a molecular weight which falls between 1,000 and 10,000 daltons.
D002465:Cell Movement / Q000187:drug effects; D006358:Hot Temperature; D006801:Humans; D007075:Immunoglobulin M / Q000378:metabolism*; D009504:Neutrophils / Q000201:enzymology / Q000378:metabolism*; D010196:Pancreatic Elastase / Q000494:pharmacology*; D010452:Peptide Biosynthesis; D010455:Peptides / Q000494:pharmacology*
D007075:Immunoglobulin M; D010455:Peptides; D010196:Pancreatic Elastase