Analysis and purification of the blood-sinusoidal domain of rat liver plasma membrane.
Methods are described for the analysis and purification of the blood-sinusoidal domains of rat liver plasma membranes using a combination of sucrose and Ficoll density gradient centrifugation. Use has been made of 125I-labelled wheat-germ agglutinin and hormone-stimulated adenylate cyclase to identify the blood sinusoidal fraction, which may be resolved from Golgi and endoplasmic reticulum markers on Ficoll gradients.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000648:ultrastructure*; D002499:Centrifugation, Density Gradient / Q000379:methods; D005260:Female; D008099:Liver / Q000648:ultrastructure*; D008102:Liver Circulation; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains