High pressure liquid chromatographic determination of nitrofurazone and furazolidone in chicken and pork tissues.
A high pressure liquid chromatographic (HPLC) procedure is presented for the determination of nitrofurazone and furazolidone in chicken and pork tissues in the 2-40 ppb range. Muscle, liver, and kidney are homogenized with cold methanol and water (50 + 50). Following methanol evaporation, the nitrofurans are partitioned into ethyl acetate and cleaned up on an alumina column. After elution with 20% methanol in ethyl acetate and evaporation to dryness, residues are determined by HPLC, using a reverse phase analytical column. Overall average recoveries for nitrofurazone and furazolidone were 65.7 and 73.5%, respectively. Average relative standard deviations of 11.9% (nitrofurazone) and 9.5% (furazolidone) at the 2 ppb level were achieved.
D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005664:Furazolidone / Q000032:analysis*; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000032:analysis; D009583:Nitrofurazone / Q000032:analysis*; D013552:Swine
D005664:Furazolidone; D009583:Nitrofurazone