Characterization and properties of a modified human interferon-alpha containing an additional 18 amino acids at the N-terminus.
A modified human interferon-alpha 2 was produced in Escherichia coli cells infected with phage M13 mp7 containing an interferon-alpha gene. After purification by immunochromatography with the monoclonal antibody NK2, the N-terminal amino acid sequence was determined. The N-terminal methionine was absent but an additional sequence of 18 amino acids at the N-terminus was retained. The modified interferon-alpha 2 was indistinguishable from authentic interferon-alpha 2 in its ability to activate natural killer cells, to slow the growth of Daudi cells, and to confer virus resistance on heterologous cells.
D000595:Amino Acid Sequence; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006801:Humans; D007370:Interferon Type I / Q000032:analysis / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000276:immunology; D014780:Viruses / Q000254:growth & development
D007370:Interferon Type I