Calcium-induced Ca2+ release from sarcoplasmic reticulum of pigs susceptible to malignant hyperthermia. The effects of halothane and dantrolene.
Calcium-induced calcium release and halothane-induced calcium release from pig sarcoplasmic reticulum (SR) were studied. The SR prepared from pigs susceptible to malignant hyperthermia (MH) was shown to release calcium at a much lower level of calcium content than in normal pig SR. The concentration above which halothane can release calcium is 40 microM for both MH-SR and normal SR, although the latter required a high calcium content to demonstrate the calcium release. Dantrolene was shown to inhibit the halothane-induced calcium release. Results suggest that SR plays an important role in pathogenesis of MH.
D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D003620:Dantrolene / Q000494:pharmacology*; D006221:Halothane / Q000494:pharmacology*; D007700:Kinetics; D008305:Malignant Hyperthermia / Q000378:metabolism*; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D013552:Swine
D003620:Dantrolene; D002118:Calcium; D006221:Halothane