Uncoupled release of protein and lipid in a protein-secreting bacterium, Bacillus brevis 47.
Bacillus brevis 47 was found to release approx. 10% of the total cellular lipids into the medium, and the protein secretion process in B. brevis 47 was studied to determine whether any relationship exists with lipid synthesis or alteration in the lipid composition. B. brevis 47 contained the usual phospholipids such as phosphatidylethanolamine, phosphatidylglycerol and cardiolipin as well as diglycerides as major neutral lipids. The extracellular lipid consisted of the same constituents as the cellular lipid but with a significantly altered composition. Divalent cations such as Ca2+, which specifically inhibit protein secretion, had no effect on the lipid release. A nonprotein-secreting mutant released lipid to the same extent as wild-type cells. Based on these results, we conclude that protein secretion occurs independently of lipid release.
D001407:Bacillus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D007700:Kinetics; D050356:Lipid Metabolism; D008055:Lipids / Q000096:biosynthesis; D010743:Phospholipids / Q000378:metabolism; D013045:Species Specificity; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D008055:Lipids; D010743:Phospholipids; D002118:Calcium