A replica technique for the evaluation of human skin by scanning electron microscopy.
A detailed reproducible procedure has been developed for the replication of human skin for scanning electron-microscopic evaluation. Utilizing a test surface, a number of negative and positive materials were evaluated for consistence and clarity of replication. Optimal replication parameters were determined and the method was successfully applied to the evaluation of human skin in vivo. Furthermore, a method was established which permitted repetitive examination of selected skin sites. Experimental results suggest that this replicating procedure can be used to monitor changes in the stratum corneum over time, and extend the range of stratum corneum magnifications.
D005898:Glass; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012117:Resins, Synthetic; D012828:Silicones; D012867:Skin / Q000648:ultrastructure*
D012117:Resins, Synthetic; D012828:Silicones