R-banding of human chromosomes by heat denaturation and Giemsa staining after amethopterin-synchronization.
Human late prophase to late metaphase chromosomes were prepared after amethopterin cell synchronization. R-banding was produced by heat denaturation followed by Giemsa staining (RHG). Haploid sets of prophase chromosomes contain approximately 850 bands. Sequences of chromosomes of different degrees of condensation are presented; their analysis provides helpful information to identify the elongated chromosomes and to follow band subdivision. The heat denaturation technique is free from most of the disadvantages encountered with R-banding by 5-bromodeoxyuridine incorporation. Giemsa stained R-bands produced by heat denaturation on prophase and prometaphase chromosomes are useful to analyse the numerous chromosome anomalies involving R-bands. In conjunction with G-banding, it is also important to compare adequately the positive and negative regions of each chromosome to define the anomalies with precision.
D001399:Azure Stains; D002871:Chromosome Banding / Q000379:methods*; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006358:Hot Temperature; D006801:Humans; D008677:Metaphase; D008727:Methotrexate; D009691:Nucleic Acid Denaturation; D011418:Prophase
D001399:Azure Stains; D008727:Methotrexate