Cloning of genomic sequences from the human Y chromosome after purification by dual beam flow sorting.
Human Y chromosomes were purified by dual beam flow sorting from a human X Chinese hamster cell line retaining the Y as the only free human chromosome. DNA was extracted from the Y fraction and cloned into lambda gtWES . lambda B vector arms. More than 100 recombinant clones carrying human inserts have been characterised by Benton-Davis plaque screening and Southern blotting or in situ hybridisation. Several repetitive sequences were found to be predominantly located on the Y, whereas the majority also cross-hybridised with autosomal DNA. One repetitive clone gave a specific hybridisation signal with the X and the Y chromosome but not with autosomes. Preliminary evidence indicates that many clones contain single copy as well as repetitive sequences. However, no Y-specific single copy sequence has yet been identified.
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D005796:Genes; D006801:Humans; D006822:Hybrid Cells / Q000648:ultrastructure; D008297:Male; D009693:Nucleic Acid Hybridization; D012730:Sex Chromosomes / Q000648:ultrastructure*; D014998:Y Chromosome / Q000648:ultrastructure*
D004274:DNA, Recombinant; D004247:DNA