title: The long-term influence of decentralisation or preganglionic hypogastric nerve stimulation in vivo on the reinnervation of minced vas deferens in the guinea-pig.
abstract: Previous studies have shown that minced regenerating smooth muscle of the guinea-pig vas deferens becomes reinnervated by nerves growing in from the surrounding intact vas deferens. Using electron microscopy, we have examined the effect of altering activity in the preganglionic nerves, either by decentralisation, or by chronic stimulation of the hypogastric nerve, in vivo, on the reinnervation of regenerating smooth muscle cells. Chronic stimulation induced earlier reinnervation than that seen in unstimulated (sham-operated) or decentralised preparations; the number of nerve profiles present in four preparations stimulated for up to 7 days was approximately 10-20 times that seen in unstimulated or decentralised preparations. However, electron micrographs revealed that "empty" nerve terminals were a feature following stimulation for longer periods. Decentralised preparations showed little change of reinnervation, at least up to 7 weeks. Compensatory changes in the density of innervation were found in the unstimulated contralateral vas deferens.
mesh_terms: D000320:Adrenergic Fibers / Q000502:physiology; D000818:Animals; D002799:Cholinergic Fibers / Q000502:physiology; D004558:Electric Stimulation; D006168:Guinea Pigs; D007001:Hypogastric Plexus / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D008854:Microscopy, Electron; D009130:Muscle, Smooth / Q000502:physiology*; D009416:Nerve Regeneration; D009469:Neuromuscular Junction / Q000502:physiology*; D014649:Vas Deferens / Q000294:innervation / Q000502:physiology