Interaction of antimycin with cytochrome b-561. A study in secretory granules and in plasma membrane isolated from chromaffin cells of bovine adrenal medulla.
Cytochrome b-561 in chromaffin granules interacts with antimycin and its alpha-peak shifts 1 nm towards red. When chromaffin granules were treated with Triton X-100 antimycin no effect was observed. Cytochrome b-561 is located in the plasma membrane isolated from the chromaffin cells. The plasma membrane b-561 does not seem to interact with antimycin. A number of NADH or NADPH (acceptor) oxidoreductase activity has been observed in isolated plasma membrane providing clues to the origin of plasma membrane dehydrogenase. The possible role of cytochrome b-561 in secretory granules other than its accredited energy conserving electron transport property is projected.
D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D000968:Antimycin A / Q000031:analogs & derivatives* / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002837:Chromaffin Granules / Q000187:drug effects / Q000378:metabolism*; D002838:Chromaffin System / Q000378:metabolism*; D003573:Cytochrome b Group / Q000378:metabolism*; D004227:Dithionite / Q000494:pharmacology; D007700:Kinetics; D010084:Oxidation-Reduction; D013053:Spectrophotometry
D003573:Cytochrome b Group; C032456:antimycin; C023646:cytochrome b561; D004227:Dithionite; D000968:Antimycin A; D001205:Ascorbic Acid