title: The primary structure of the glycan moiety of pseudomurein from Methanobacterium thermoautotrophicum.
abstract: After treatment of isolated cells walls of Methanobacterium thermoautotrophicum with sodium hydroxide or anhydrous hydrazine, water soluble glycan strands were obtained. These consisted of alternating (beta 1-3)-linked D-glucosamine and (alpha 1-3)-linked L-talosaminuronic acid residues and their length was about 25 disaccharides. Some of the L-talosaminuronic acid residues remained linked to either glutamic acid or the peptides N-gamma-glutamylalanine and N epsilon-(gamma-glutamylalanyl)lysine, indicating that the peptide moiety of pseudomurein is bound to the carboxyl group of talosaminuronic acid via the amino group of glutamic acid.
mesh_terms: D002236:Carbohydrate Conformation; D055598:Chemical Phenomena; D002621:Chemistry; D002627:Chemistry, Physical; D004187:Disaccharides / Q000032:analysis; D019605:Euryarchaeota / Q000032:analysis*; D006868:Hydrolysis; D007700:Kinetics; D010457:Peptidoglycan / Q000032:analysis*; D011134:Polysaccharides / Q000032:analysis*
chemical_list: D004187:Disaccharides; D010457:Peptidoglycan; D011134:Polysaccharides