Biosynthesis of lung lipids from acetoacetate and glucose in the developing rat in vivo.
1. [3-14C]Acetoacetate and [U-14C]glucose administered to rats were readily utilized for synthesis of phospholipids, cholesterol and glycerides in the lung during suckling period. 2. Among all lipids synthesized, phosphatidylcholine accounted for 31-48% from [14C]acetoacetate and 30-46% from [14C]glucose. The synthesized phosphatidylcholine contained predominantly [14C]-labeled palmitate. 3. Glycerol lipids represented 83-92% and 56-68% of total lipids synthesized from [14C]glucose and [14C]acetoacetate, respectively. 4. Glucose is the preferred substrate for glyceride-glycerol, while acetoacetate appears better for fatty acid and cholesterol synthesis.
D000090:Acetoacetates; D000367:Age Factors; D000818:Animals; D002784:Cholesterol / Q000096:biosynthesis; D005230:Fatty Acids, Nonesterified / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism*; D007651:Keto Acids / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis*; D008168:Lung / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000096:biosynthesis; D011663:Pulmonary Surfactants / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D000090:Acetoacetates; D005230:Fatty Acids, Nonesterified; D007651:Keto Acids; D008055:Lipids; D010713:Phosphatidylcholines; D011663:Pulmonary Surfactants; C016635:acetoacetic acid; D002784:Cholesterol; D005947:Glucose