Presence and absence of virus particles in hybridomas secreting monoclonal antibodies against gliomas.
Hybridoma clones were produced by fusion of splenocytes from glioma-immunized hosts and the X63-Ag8.653 mouse myeloma line and Y3-Ag.1.2.3. rat myeloma line. Oncornavirus particles were found in all clones descending from the mouse myeloma line. No virus particles could be found in either the spleens of immunized Balb/c mice and Fischer rats or in the rat myeloma line and the hybridomas derived from it.
D000818:Animals; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm / Q000032:analysis; D001932:Brain Neoplasms / Q000276:immunology / Q000382:microbiology*; D005910:Glioma / Q000276:immunology / Q000382:microbiology*; D006825:Hybridomas / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000276:immunology / Q000382:microbiology; D051381:Rats; D011916:Rats, Inbred F344; D012190:Retroviridae / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm