Metabolism of leukotriene D by porcine kidney.
An enzyme from porcine kidney converted leukotriene D4 into a less polar metabolite. The structure of this compound was 5-hydroxy-6-S-cysteinyl-7,9-trans-11,14-cis-eicosatetraenoic acid (leukotriene E4). Analogous products, viz. 5-hydroxy-6-S-cysteinyl-7,9,11-eicosatrienoic acid (leukotriene E3), 5-hydroxy-6-S-cysteinyl-7,9,11-trans-14-cis-eicosatetraenoic acid (11-trans-leukotriene E4), and 5-hydroxy-6-S-cysteinyl-7,9,11,14,17-eicosapentaenoic acid (leukotriene E5) were formed from leukotrienes D3, 11-trans-D4, and D5, respectively. Leukotriene E4 induced slow reacting substance-like contractions of guinea pig ileum but was less potent (8-12 times) than leukotriene C4. The biological potency of 11-trans-leukotriene E4 was similar to that of leukotriene E4.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism* / Q000494:pharmacology; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D004150:Dipeptidases / Q000378:metabolism; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D007668:Kidney / Q000378:metabolism*; D009119:Muscle Contraction / Q000187:drug effects; D013189:SRS-A / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D013552:Swine
D001095:Arachidonic Acids; D013189:SRS-A; D004150:Dipeptidases