[Monoclonal antibodies against surface antigens of cancer cells. Experimental preparation for early diagnosis and immuno-assisted therapy].
Humoral and cellular immune responses to laryngeal carcinomas suggest the presence of tumor associated antigens in the surface of larynx carcinoma cells. Production of antisera against specific antigens on human tumors or tissues is complicated by the concomitant production of antibodies that react with all human cells. To circumvent this problem monospecific antibodies needed. After isolation of larynx carcinoma cells they were used to immunize mice. From these mice the antibody producing spleen cells were isolated and fused with myeloma cells resulting in antibody secreting hybrid cells. A hybridoma clone was isolated producing antibodies that did not bind to various normal human cells nor to cells of non human origin. In contrast, the monoclonal antibody was able to identify various malignant target cells, including two laryngeal carcinomas and two salivary gland carcinomas.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000175:diagnosis / Q000276:immunology* / Q000628:therapy; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D008565:Membrane Proteins
Monoklonale Antikörper gegen Oberflächen-Antigene von Karzinomzellen. Ein experimenteller Ansatz zur Frühdiagnose und immunassistierten Therapie.