title: Dietary chromium - forms and availabilities.
abstract: The chromium naturally occurring in plants eluted from Sephadex G-25 at approximately 2600 D. Total chromium was quantitated with flameless atomic absorption spectrophotometry. A plant ligand tagged with radioactive chromium both in vivo and in vitro migrated on Sephadex G-25 identically to the naturally occurring chromium compound. The molecular weight of the radioactively tagged chromium compound was 2600 daltons on Sephadex G-25. Similar complexes isolated from plant species were found attached to an organic ligand. The ligand appears to have 2 components, differing in composition by an amine group. This extremely stable (KD = 9 X 10(-5)anionic complex does not contain peptide or deoxyribose units. When alfalfa was exposed to either Cr(III) or Cr(VI), only Cr(III) was isolated in this organic chromium compound. The alfalfa bioreduction system can be saturated, as evidenced by Cr(VI) isolation of ionic in those plant extracts incubated with high levels of Cr(VI) in vitro. The gastrointestinal chromium physiology studies show that the radioactively labelled plant chromium compounds remained intact through the gastrointestinal tract up to the large intestine. Some degradation products were identified in the rat cecum. Approximately 30% of the plant chromium available to the rat was absorbed across the gastrointestinal tract.
mesh_terms: D000818:Animals; D002857:Chromium / Q000032:analysis*; D002860:Chromium Radioisotopes; D004032:Diet; D005243:Feces / Q000032:analysis; D005260:Female; D005504:Food Analysis; D006801:Humans; D000455:Medicago sativa / Q000032:analysis; D010944:Plants / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013054:Spectrophotometry, Atomic / Q000379:methods
chemical_list: D002860:Chromium Radioisotopes; D002857:Chromium