[Analysis of sister chromatid exchanges in the 1st, 2d and 3d mitoses using 5-bromodeoxyuridine and 5-bromodeoxycytidine].
The cultures of Chinese hamster cells were treated with different concentrations (2.5, 5.0, 10.0, 20.0, 80.0 and 160.0 microgram/ml) 5-BrdU and 5-BrdC during 12 hours. The cultures were fixed at the 24-th hour. The linear increase of sister chromatid exchanges (SCE) was discovered with the increase of BrdU concentration. No change of SCE frequency was observed at different BrdC concentrations. The reasons for these differences in a concentration effect are discussed.
D000818:Animals; D001972:Bromodeoxycytidine / Q000494:pharmacology*; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003434:Crossing Over, Genetic / Q000187:drug effects*; D003841:Deoxycytidine / Q000031:analogs & derivatives*; D004305:Dose-Response Relationship, Drug; D008938:Mitosis / Q000187:drug effects*; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013997:Time Factors
D003841:Deoxycytidine; D001972:Bromodeoxycytidine; D001973:Bromodeoxyuridine
Analiz sestrinskikh khromat dnykh obmenov v pervom, vtorom i tret'em mitozakh s pomoshch'iu 5-bromdezoksiuridina i 5-bromdezoksitsitidina.