Glucuronidation in isolated perfused rat intestinal segments after mucosal and serosal administration of 1-naphthol.
Glucuronidation of 1-naphthol in the rat intestinal wall was studied in isolated perfused intestinal segments. 1-Naphthol glucuronide appeared on the mucosal as well as on the serosal side of the intestinal segments irrespective of the side (mucosal and/or serosal) on which naphthol was administered. Appearance of naphthol glucuronide was linear with time. A lag phase of 3.9 +/- 0.9 and 4.3 +/- 1.0 min was observed for the appearance on the mucosal and serosal side, respectively. Total glucuronide appearance rate after serosal administration of 10 to 100 microM naphthol was 3- to 4-fold higher than after mucosal administration. It is concluded that glucuronidation after mucosal administration is limited by the entry of naphthol into the metabolizing compartment.
D000818:Animals; D001692:Biological Transport; D005965:Glucuronates / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009284:Naphthols / Q000494:pharmacology*; D010477:Perfusion; D051381:Rats
D005965:Glucuronates; D009284:Naphthols; C029350:1-naphthol