[Reversal by isaxonine of the inhibitory effect of vincristine on the growth of neurites in cultures (author's transl)].
It has been previously demonstrated that isaxonine increases neurite elongation of dorsal root ganglia in vitro. In purpose to demonstrate that this effect results of an action at the level of the cellular cytoskeleton, we have studied effects of isaxonine on neurite elongation inhibited by vincristine sulfate which is known to bind specifically on tubulin and consequently pertube microtubule assembly. Neurite elongation of dorsal root ganglia cultivated with vincristine (0,5 or 1,0 10(-7) M) is compared to neurite elongation of ganglia cultivated with vincristine plus isaxonine (10(-7) M). Results show that isaxonine prevents, at least partially, the effect of vincristine. The protective effect observed in vivo in the rat and in the human seems to indicate that isaxonine acts at the level of microtubules of nerve fiber.
D000818:Animals; D001369:Axons / Q000187:drug effects*; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000187:drug effects / Q000254:growth & development*; D066298:In Vitro Techniques; D051379:Mice; D008870:Microtubules / Q000378:metabolism; D011743:Pyrimidines / Q000494:pharmacology*; D014404:Tubulin / Q000378:metabolism; D014750:Vincristine / Q000037:antagonists & inhibitors* / Q000494:pharmacology
D011743:Pyrimidines; D014404:Tubulin; D014750:Vincristine; C020375:isaxonine
Réversion par l'isaxonine de l'action inhibitrice de la vincristine sur la croissance des neurites en culture.