Failure to demonstrate retrograde labelling of cerebellar Purkinje cells after injection of [3H]GABA in Deiters' nucleus.
After microinjections of [3H]GABA into the dorsal part of the Deiters' nucleus no retrograde labelling could be observed in the cerebellar Purkinje cells projecting to this nucleus. Tentatively, this failure to demonstrate transmitter specific retrograde labelling is attributed to lack of high affinity uptake mechanisms in the GABAergic Purkinje axon terminals. These observations indicate a very important role of terminal uptake mechanisms in initiation of transmitter specific retrograde labelling. Anterograde axonal labelling was observed along several tracts originating in the injected area, but was not limited to connections with GABA as likely transmitter candidate.
D000818:Animals; D001345:Autoradiography; D001931:Brain Mapping / Q000379:methods*; D005260:Female; D009434:Neural Pathways / Q000378:metabolism; D011689:Purkinje Cells / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014726:Vestibular Nuclei / Q000378:metabolism*; D003689:Vestibular Nucleus, Lateral / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D005680:gamma-Aminobutyric Acid