title: Isolation and properties of a lectin from the seeds of the Indian bean or lablab (Dolichos lablab L.).
abstract: The lectin of the Indian bean or lablab (Dolichos lablab L.) was purified by affinity chromatography on two types of affinity carriers: O-alpha-D-mannopyranosyl-Separon and Separon-bound ovomucoid. The lectin is homogeneous in the ultracentrifuge: S20, w = 6.14 S, Mr = 110 000; the molecule appears to comprise two pairs of two types of subunits (Mr 16 000 and 40 000), and contains 2% neutral sugar and 0.2 Mn and 0.5 Zn atom respectively. The lectin agglutinates human erythrocytes non-specifically with regard to ABO grouping at a limit concentration of 8 micrograms/ml, and this activity is inhibited most effectively by N-acetyl-D-glucosamine, methyl alpha-D-mannopyranoside and ovomucoid, but not by free D-mannose.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000032:analysis*; D006385:Hemagglutination Inhibition Tests; D037102:Lectins / Q000302:isolation & purification* / Q000494:pharmacology; D037121:Plant Lectins; D010946:Plants, Medicinal; D012639:Seeds
chemical_list: D000596:Amino Acids; D037102:Lectins; D037121:Plant Lectins