In-vitro fertilization of zona-free bovine oocytes matured in culture.
Bovine oocytes removed from 3-5 mm follicles were matured in vitro for 22-24 h. The zonae pellucidae were then dissolved by pronase and the eggs were transferred to droplets of preincubated bovine epididymal spermatozoa. Of 575 oocytes, 510 (88.7%) were fertilized and in 261 (45.4%) of them normal male and female pronuclei were present. In the rest male pronucleus formations were arrested or were undetermined (9.7%). Approximately half of the oocytes were fertilized by greater than or equal to 2 spermatozoa. In some of the polyspermic cells male pronuclei of normal size were accompanied by less advanced stages, including sperm heads at the beginning of decondensation. We conclude that a cytoplasmic substance responsible for male pronucleus formation is present in about 50% of randomly selected bovine oocytes.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D008722:Methods; D009865:Oocytes / Q000254:growth & development*; D010063:Ovum / Q000254:growth & development*; D011402:Pronase; D013084:Sperm-Ovum Interactions; D015044:Zona Pellucida
D011402:Pronase