Effect of 1,25-dihydroxycholecalciferol on the duodenal villi and alkaline phosphatase in the developing chick embryo.
Administration of 1,25-(OH)2D3 to developing 14-day chick embryo gave precocious induction of alkaline phosphatase in 20-day chick embryonic duodenum. 1,25-(OH)-2D3-induced alkaline phosphase involved in changes in Km and Vmax values. Furthermore, polyacrylamide gel disc electrophoresis of n-butanol-solubilized alkaline phosphatase from control and 1,25-(OH)2D3-treated chick embryonic duodenum revealed that 1,25-(OH)2D3 involved the transformation of neuraminidase-resistant fast migrating form to the neuraminidase-sensitive faster migrating one. Scanning electron microscopic data showed that the injection of 1,25-(OH)2D3 stimulated the elongation of duodenal microvilli, although there was no effect on the duodenal absorptive epithelial cell height.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002117:Calcitriol; D002462:Cell Membrane / Q000201:enzymology*; D002642:Chick Embryo; D004100:Dihydroxycholecalciferols / Q000494:pharmacology*; D004386:Duodenum / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D006887:Hydroxycholecalciferols / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000201:enzymology*; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008871:Microvilli / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure
D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols; D007527:Isoenzymes; D000469:Alkaline Phosphatase; D002117:Calcitriol