Gas-liquid chromatography-frequency pulse-modulated electron-capture detection in the diagnosis of infectious diseases.
The extremely sensitive and selective gas-liquid chromatography-frequency pulsed-modulated electron-capture detection (GLC-FP-ECD) procedure has been applied to the diagnosis of bacterial, fungal, viral, rickettsial, and parasitic diseases by the examination of various body fluids, effusions and exudates, and excretion products. Carboxylic acid and alcohol, hydroxy acid, and amine product profiles of microbial or host-response origin, have been used to establish specific etiologies, these profiles are reproducible, and can be used to aid in the diagnosis of infections. In addition, we have used the GLC-FP-ECD procedure to analyze microbial metabolic products in vitro and to provide data for identification and classification. We also explored computer time-sharing for data analysis, profile library comparison, and eventual profile matching for diagnosis.
D000438:Alcohols / Q000032:analysis; D001419:Bacteria / Q000145:classification / Q000378:metabolism*; D001424:Bacterial Infections / Q000097:blood / Q000382:microbiology*; D001826:Body Fluids / Q000032:analysis* / Q000382:microbiology; D002849:Chromatography, Gas; D003201:Computers; D005023:Ethylene Chlorohydrin / Q000031:analogs & derivatives; D005227:Fatty Acids / Q000032:analysis; D005466:Fluorocarbons; D006801:Humans; D006880:Hydroxy Acids / Q000032:analysis; D010996:Pleural Effusion / Q000032:analysis; D013045:Species Specificity
D000438:Alcohols; D005227:Fatty Acids; D005466:Fluorocarbons; D006880:Hydroxy Acids; C007924:heptafluorobutyric anhydride; D005023:Ethylene Chlorohydrin; C005849:2,2,2-trichloroethanol