Specific and reversible activation and inactivation of the mitochondrial phosphate carrier by cardiolipin and nonionic detergents, respectively.
The phosphate carrier of pig heart mitochondria was solubilized with Triton X-100 and purified by chromatography on hydroxylapatite. Incubation of the phosphate carrier fraction with cardiolipin stimulated the reconstituted [32P]phosphate exchange activity in liposomes, whereas increased Triton X-100 concentrations inhibited it. The effects of cardiolipin and Triton X-100 are reversible. The activation by cardiolipin is highly specific and could not be obtained with any other applied phospholipid.
D000818:Animals; D002308:Cardiolipins / Q000494:pharmacology*; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002417:Cattle; D003902:Detergents / Q000494:pharmacology*; D008929:Mitochondria, Heart / Q000378:metabolism*; D017830:Octoxynol; D028044:Phosphate-Binding Proteins; D011092:Polyethylene Glycols / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology*; D013552:Swine
D002308:Cardiolipins; D002352:Carrier Proteins; D003902:Detergents; D028044:Phosphate-Binding Proteins; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D017830:Octoxynol