title: Anesthesia and surgical trauma: their influence on the guinea pig compound action potential.
abstract: A chronic implant consisting of a fine thermocouple placed on the round window permitted measurement of temperature and of the compound action potential (CAP) of the cochlear nerve in guinea pigs. Thresholds and latencies of the CAP, in response to tone bursts (2-40 kHz), were measured when the animal was awake and unrestrained, and again after several hours of anesthesia. The CAP remained unchanged with a variety of common anesthetics when precise control of round window temperature was maintained. However, when anesthesia was accompanied by several hours of slight cochlear cooling, thresholds were elevated for CAPs evoked by frequencies above 24 kHz and latencies were increased for CAPs evoked by all frequencies tested. The effects of surgery on CAP threshold and latency were also examined. Guinea pigs were tested while still anesthetized at the conclusion of the implantation procedure, and then again several days later while awake. Thresholds and latencies were unchanged. In two anesthetized guinea pigs already implanted with thermocouples, ventral and post-auricular surgery to expose the middle ear had no effect on the CAP, when low-speed drilling was used to open the auditory bulla. However, when small portions of the bulla were broken away with forceps, the CAP in response to high-frequency tone bursts immediately showed elevated thresholds. This alteration of the CAP was clearly different from that produced by lowered temperature, since the latencies at threshold were significantly decreased.
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000777:Anesthetics; D000818:Animals; D001309:Auditory Threshold / Q000187:drug effects; D001831:Body Temperature; D003056:Cochlear Nerve / Q000187:drug effects / Q000502:physiology*; D004423:Ear / Q000601:surgery*; D006168:Guinea Pigs; D011930:Reaction Time / Q000187:drug effects; D012405:Round Window, Ear / Q000502:physiology
chemical_list: D000777:Anesthetics