Does de novo synthesis of lysophosphatidylcholine occur in rat lung microsomes?
Incubation of rat lung microsomes with CDP[Me-14C]choline resulted in formation of radioactive lysophosphatidylcholine and phosphatidylcholine. Evidence is provided which suggests that lysophosphatidylcholine formation cannot be ascribed completely to phospholipase. A degradation of phosphatidylcholine. Lysophosphatidylcholine production can be stimulated by addition of monoacylglycerol or diacylglycerol. It is suggested that diacylglycerol is partly hydrolyzed to monoacylglycerol and subsequently converted to lysophosphatidylcholine. A direct transfer of phosphocholine from CDPcholine to monoacylglycerol is demonstrated by equimolar incorporation of 1(3)-[9,10-3H2]palmitoylglycerol and phospho[Me-14C]choline into lysophosphatidylcholine.
D000818:Animals; D004075:Diglycerides / Q000494:pharmacology; D005989:Glycerides / Q000494:pharmacology; D066298:In Vitro Techniques; D008168:Lung / Q000378:metabolism* / Q000648:ultrastructure; D008244:Lysophosphatidylcholines / Q000096:biosynthesis*; D008861:Microsomes / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000096:biosynthesis / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism; D011663:Pulmonary Surfactants / Q000096:biosynthesis; D051381:Rats
D004075:Diglycerides; D005989:Glycerides; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D011663:Pulmonary Surfactants; D010741:Phospholipases A