Determination of serum iron and the total iron-binding capacity with a new chromogen, 4-aminophenazone.
A colorimetric procedure is described for the determination of serum iron and TIBC, utilizing the action of the iron on the oxidation reaction of 4-aminophenazone with H2O2. The chromogen oxidized has maximum absorption at 540 nm. The method is interesting because it has important advantages over other methods: no deproteinization is needed, microsamples are used, it is simple, gives adequate precision, and the results correlate well with those obtained with the standard bathophenanthroline method.
D003300:Copper / Q000494:pharmacology; D006801:Humans; D007501:Iron / Q000097:blood*; D008274:Magnesium / Q000494:pharmacology; D010783:Photometry / Q000379:methods*; D011721:Pyrenes
D011721:Pyrenes; D003300:Copper; D007501:Iron; D008274:Magnesium; C004482:1-aminopyrene