The measurement of nanograms of tocopherol from needle aspiration biopsies of adipose tissue: normal and abetalipoproteinemic subjects.
A method for the analysis of tocopherol in human adipose tissue using high performance liquid chromatography and fluorescence spectrometry is described; results are expressed relative to total triglyceride content measured by the reaction of the methylated fatty acids with hydroxylamine and ferric chloride. The tocopherol contents of adipose tissue obtained at surgery and by the needle aspiration biopsy technic of ambulatory human subjects (who did not take supplemental vitamin E) were found to be virtually identical. The tocopherol content of adipose tissue by the needle aspiration technic was 262 +/- 33 ng tocopherol/mg triglyceride; this value was increased twofold or more in persons ingesting additional vitamin E. Patients with abetalipoproteinemia (ABL) who absorb tocopherol poorly and have extremely low levels of plasma and red blood cell tocopherol also had a low concentration of adipose tissue tocopherol. However, some ABL patients on massive supplementation with vitamin E (approximately 10 g daily) did achieve normal concentrations of adipose tissue tocopherol.
D000012:Abetalipoproteinemia / Q000188:drug therapy / Q000378:metabolism*; D000273:Adipose Tissue / Q000032:analysis*; D000328:Adult; D001707:Biopsy, Needle; D002648:Child; D002675:Child, Preschool; D002784:Cholesterol / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008832:Microchemistry; D013050:Spectrometry, Fluorescence; D014810:Vitamin E / Q000032:analysis* / Q000627:therapeutic use
D005227:Fatty Acids; D014810:Vitamin E; D002784:Cholesterol