title: Membrane dynamics in human leukemia and lymphoma cells. pH dependency of diphenylhexatriene fluorescence polarization.
abstract: Membrane dynamics of human leukemia and lymphoma cell lines were analyzed by investigating the effect of pH on fluorescence polarization (P) of the lipophilic probe diphenylhexatriene (DPH). The degree of P varied as a function of pH, depending on the cell lines. These variations were not detected in phospholipid vesicles. In addition, they were prevented by treatments with glutaraldehyde, sodium azide or phenylmethylsulfanyl fluoride, a specific protease inhibitor. Therefore, these P value changes might be influenced by protein modification.
mesh_terms: D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004161:Diphenylhexatriene; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007938:Leukemia / Q000378:metabolism*; D008223:Lymphoma / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D011090:Polyenes; D013050:Spectrometry, Fluorescence
chemical_list: D008563:Membrane Lipids; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D011090:Polyenes; D004161:Diphenylhexatriene