Tracing of motoneurones and primary afferent projections after intracellular staining with Lucifer Yellow: dye-coupling.
Intracellular injection of the fluorescent dye Lucifer Yellow CH into single motoneurones of the isolated perfused frog spinal cord resulted in backfilling of presynaptic fibres originating from dorsal roots and ventrolateral funiculi. The dye transfer from primary sensory fibres into motoneurones was observed following application of Lucifer Yellow to the central end of the cut dorsal root. The dye-coupling coincides with electrical coupling at sensory-motor synapses presumably through gap junctions. The fluorescent primary afferent fibres were traced from the dorsal roots to the motor nucleus where they terminate the chains of swellings. Most swellings are located in dorsal horn and in the intermediate zone approximately 100-100 micrometers from the somata of motoneurones. A few varicosities are located ion the cell bodies of the motoneurones.
D000344:Afferent Pathways / Q000033:anatomy & histology; D000818:Animals; D003712:Dendrites / Q000648:ultrastructure; D005727:Ganglia, Spinal / Q000033:anatomy & histology; D007546:Isoquinolines; D008856:Microscopy, Fluorescence; D009046:Motor Neurons / Q000648:ultrastructure*; D009412:Nerve Fibers / Q000648:ultrastructure; D011895:Rana ridibunda; D013116:Spinal Cord / Q000033:anatomy & histology*; D013569:Synapses / Q000648:ultrastructure
D007546:Isoquinolines; C017475:lucifer yellow