G0-G1 transition of rat hepatocytes detected by changes in nuclear chromatin condensation after in vivo treatment with phenobarbital.
The transition of hepatocytes from G0 to late G1-phase was studied in phenobarbital (PB) treated rats using visual and cytofluorometric evaluations of nuclear fluorescence patterns after quinacrine dihydrochloride (QDH) staining. In controls, about 90% of the nuclei showed bright nuclear fluorescence indicating G0-phase. After 10 days there was a reduction in fluorescence intensity which became more marked with continued PB treatment, indicating a shift from a resting G0 state to a later G1 phase of the cell cycle. The relevance of these findings for the understanding of tumor promoting properties of PB is discussed.
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000502:physiology; D002843:Chromatin / Q000187:drug effects / Q000502:physiology*; D005434:Flow Cytometry; D007399:Interphase / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008856:Microscopy, Fluorescence; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D002843:Chromatin; D010634:Phenobarbital