title: Interaction between ether glycerophospholipid vesicles and serum proteins in vitro.
abstract: The effect of blood serum on the stability of small unilamellar vesicles consisting of 1-O-(1'-alkenyl)-2-acyl-sn-glycerophosphocholine (choline plasmalogen) or of the alkylacyl-, dialkyl- and diacyl analogs was evaluated by measuring either release of entrapped calcein or transfer of phospholipids from vesicles to serum high-density lipoproteins. The following order of stability was found: alkenyloleoylGPC greater than dioleoylGPC greater than di-O-octadecenylGPC greater than acyloleoylGPC = egg phosphatidylcholine = alkyloleoylGPC. AlkyloleoylGPC and acyloleoylGPC had aliphatic chain compositions similar to that of alkenyloleoylGPC. From the results obtained it is concluded that stability of vesicles in the presence of serum depends on vesicle size (larger vesicles are more stable) and on the type of bond (ether or ester) in position 2 of glycerol. Dioctadecenyl vesicles are about the same size as alkylacylGPC vesicles, but are significantly more stable in the presence of serum. Thus, it appears that an ester bond in position 2 of glycerol (which is replaced by an ether bond in dioctadecenylglycerol) favors the interaction of phospholipids with serum high-density lipoproteins or lipid-exchange proteins. The addition of cholesterol greatly enhances vesicle stability; among the vesicles used in this study those composed of alkenylacylGPC plus 30 mol% cholesterol were most resistant to disruption by serum. Experiments with sn-1 and sn-3 enantiomers of alkylacylGPC and diacylGPC have shown that interaction of vesicle membranes with serum components is independent of the steric configuration of vesicle phospholipids.
mesh_terms: D001798:Blood Proteins / Q000378:metabolism*; D002250:Carbon Radioisotopes; D006801:Humans; D007700:Kinetics; D008074:Lipoproteins / Q000097:blood*; D008081:Liposomes; D010743:Phospholipids / Q000097:blood; D010955:Plasmalogens / Q000097:blood*; D013329:Structure-Activity Relationship; D014316:Tritium
chemical_list: D001798:Blood Proteins; D002250:Carbon Radioisotopes; D008074:Lipoproteins; D008081:Liposomes; D010743:Phospholipids; D010955:Plasmalogens; C032459:choline plasmalogens; D014316:Tritium