Human placenta as a convenient source of creatine kinase BB.
We demonstrate that human placenta is a convenient source of creatine kinase isoenzyme BB. We compare the physicochemical and catalytic properties with those of other creatine kinase isoenzymes: purified human abdominal muscle MM and brain BB. We also describe a stabilizing medium for creatine kinase BB. Human placental and brain BB have similar catalytic properties, the respective Km values for creatine phosphate being 0.66 and 0.56 mmol/L and for adenosine diphosphate 89 and 70 nmol/L.
D000244:Adenosine Diphosphate; D001921:Brain / Q000201:enzymology; D003402:Creatine Kinase / Q000302:isolation & purification*; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D006801:Humans; D007527:Isoenzymes; D007700:Kinetics; D008274:Magnesium; D009132:Muscles / Q000201:enzymology; D010725:Phosphocreatine; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D014462:Ultrafiltration
D007527:Isoenzymes; D010725:Phosphocreatine; D000244:Adenosine Diphosphate; D003402:Creatine Kinase; D008274:Magnesium