Internal motion of F-actin in 10(-6)-10(-3) s time range studied by transient absorption anisotropy: detection of torsional motion.
By means of laser flash photolysis, the transient absorption anisotropy (TAA) of the triplet probe, 5-iodoacetamide-Eosin, labeling rabbit skeletal F-actin was measured in the 10(-6)-10(-3) s time range. The TAA curve at 20 degrees C showed a relatively slow decay phase covering several hundred microseconds and a large residual anisotropy (approximately 0.1 at 2 ms). After analysis with Barkley & Zimm's formula, it was concluded that the TAA of Eosin-F-actin can be approximated by the anisotropy decay due to torsional motion of F-actin.
D000199:Actins; D000818:Animals; D066298:In Vitro Techniques; D007700:Kinetics; D046911:Macromolecular Substances; D009038:Motion; D011485:Protein Binding; D011817:Rabbits; D013057:Spectrum Analysis / Q000379:methods; D014335:Tropomyosin; D014336:Troponin
D000199:Actins; D046911:Macromolecular Substances; D014335:Tropomyosin; D014336:Troponin