Removal of wheat-germ agglutinin increases protein synthesis in wheat-germ extracts.
Affinity chromatography of wheat germ extracts on a chitin column increased the rate and extent of protein synthesis, programmed by rabbit globin mRNA. Addition of purified wheat germ agglutinin to the chitin-treated extract reduced the rate of protein synthesis to about the levels seen in the untreated extracts. Experiments where the ratio of messenger to extract and the ratio of supernatant to ribosomes were varied, indicated that addition of wheat germ agglutinin reduced the amount of available ribosomes. Reduced and carboxymethylated wheat germ agglutinin failed to inhibit protein synthesis and was unable to bind to the ribosomes. However, labelled intact agglutinin was found to be bound to ribosomes. The bound agglutinin was not released by acid treatment. The inhibiting effect of wheat germ, agglutinin on protein synthesis could not be counteracted by addition of N-acetyl-D-glucosamine or sialic acid, whereas thiols partially diminished the inhibition. The data indicate that wheat germ agglutinin binds reversibly to ribosomes, probably through mixed disulfide formation, and that chitin treatment increases the ability of wheat germ extracts to support protein synthesis, at least in part, by removing the wheat germ agglutinin. The possibility that chitin treatment also removed other inhibitors of protein synthesis cannot be excluded.
D000818:Animals; D005914:Globins / Q000235:genetics; D006384:Hemagglutination; D007700:Kinetics; D037102:Lectins / Q000302:isolation & purification; D037121:Plant Lectins; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D014908:Triticum / Q000378:metabolism; D014909:Wheat Germ Agglutinins
D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; D012333:RNA, Messenger; D014909:Wheat Germ Agglutinins; D005914:Globins