Human cancer-associated gangliosides defined by a monoclonal antibody (IB9) directed to sialosyl alpha 2 leads to 6 galactosyl residue: a preliminary note.
A new monoclonal antibody (IB9) was prepared by hybridoma technique directed specifically to sialosyl alpha 2 leads to 6 galactosyl residue. With this reagent, accumulation of two major gangliosides in human colonic and liver adenocarcinoma has been detected, and these gangliosides were isolated and characterized as structures A and B (below). Another ganglioside with a ceramide nonasaccharide structure, which reacted to anti-X-hapten antibody after desialylation, was also isolated and partially characterized. (formula; see text) These gangliosides were absent or present in very small quantity in normal tissue and may represent human cancer-associated markers.
D000230:Adenocarcinoma / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000918:Antibody Specificity; D055598:Chemical Phenomena; D002621:Chemistry; D003110:Colonic Neoplasms / Q000378:metabolism*; D005732:Gangliosides / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007120:Immunochemistry; D008113:Liver Neoplasms / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D005732:Gangliosides