Biochemical studies of immune complexes. II. Purification of immune complexes from sera of patients with malignant melanoma.
Circulating immune complexes (CICs) from serum samples of patients with malignant melanoma were isolated in high yield utilizing a bifunctional chromatography matrix that binds all normal serum proteins except IgG-containing immune complexes (ICs), IgG, and transferrin. Protein fractions were subsequently analyzed by polyacrylamide gel electrophoresis (PAGE) and characterized by molecular weight. By this method, one common and several unique component nonimmunoglobulin proteins of the ICs were identified. These proteins possess similar molecular weights to several previously described human melanoma antigens. This technique should permit biochemical and immunological characterization of the component antigens of CICs in melanoma and other malignancies.
D000936:Antigen-Antibody Complex / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D004983:Ethanolamines; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D007202:Indicators and Reagents; D008545:Melanoma / Q000276:immunology*; D008970:Molecular Weight; D011863:Radioimmunoassay; D012878:Skin Neoplasms / Q000276:immunology*; D014227:Triazines
D000936:Antigen-Antibody Complex; D004983:Ethanolamines; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D014227:Triazines; C038830:DEAE affigel blue; C007369:2-diethylaminoethanol