Study of murine lymphoid cells in situ after stimulation with Concanavalin A.
The effects of Concanavalin A (Con A) on the lymphocytes in situ were investigated here by studying the blastogenesis and organization of lymphoid cells in the histological preparations of the spleen and the mesenteric lymph node and the cell death at different hours after iv administration of different doses (10, 20 and 50 micrograms/animal) of the substance. The gradual transformation of the lymphocytes into blasts was observed in the histological preparations of the spleen and the mesenteric lymph node. The rate of the change became higher with higher dose of Con A. It seems that the lymph node was affected more with in vivo Con A treatment than the spleen on account of higher percentage of blast transformation at early hour and lower density of the cells due to cell migration or cell death. Purpose of this study has been discussed.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology*; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008643:Mesentery / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology
D003208:Concanavalin A