Equimolar proportions of somatic-type core histones in chromatin of spermatogonia.
[3H]Leucine incorporation into histones of seminiferous epithelial cells of hypophysectomized rats was used to calculate the molar proportions of the core histones of spermatogonia. The molar proportions H3:H2B:(H2A + protein A24):H4 are 1:1:1:1, viz. identical with those reported by others for somatic cells. Similar results were obtained when molar proportions of histones of seminiferous epithelial cells from immature rat testis (predominantly populated with spermatogonia) were determined by the dye-binding method. These data are relevant to mechanisms for the replacement of some of the core histones by variants during the primary spermatocyte stages.
D000818:Animals; D002843:Chromatin / Q000032:analysis*; D006657:Histones / Q000032:analysis* / Q000096:biosynthesis; D007016:Hypophysectomy; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D051381:Rats; D012671:Seminiferous Tubules / Q000502:physiology; D013093:Spermatogonia / Q000032:analysis*; D013094:Spermatozoa / Q000032:analysis*; D014316:Tritium
D002843:Chromatin; D006657:Histones; D014316:Tritium; D007930:Leucine