Visualization of skin appendages by scanning electron microscopy.
A method for SEM visualization of skin appendages and elastic fibres around them is described. Skin sections were autoclaved for 4 h and followed by fixation, dehydration and xylol-air drying. Using this method, the three-dimensional architectural details of each type of skin appendage and related elastic fibre network were demonstrated.
D000328:Adult; D004439:Eccrine Glands / Q000648:ultrastructure; D006197:Hair / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008875:Middle Aged; D012627:Sebaceous Glands / Q000648:ultrastructure; D012867:Skin / Q000648:ultrastructure*