title: Isolation and characterization of profilactin and profilin from calf thymus and brain.
abstract: 1. Profilactin and profilin have been purified from calf thymus and calf brain. Thymus profilactin could be crystallized with a similar technique as described earlier for the spleen protein. 2. Preparations of profilactin from the three calf tissues spleen, thymus and brain contain a mixture of beta actin and gamma actin. The ratio beta/gamma differs between the tissues, but is constant throughout the purification steps. 3. The properties of profilin isolated from three sources (spleen, thymus and brain) indicate that it is a highly conserved protein.
mesh_terms: D000199:Actins / Q000302:isolation & purification*; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002850:Chromatography, Gel; D003285:Contractile Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D008840:Microfilament Proteins; D051304:Profilins; D011506:Proteins / Q000302:isolation & purification*; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate; D013950:Thymus Gland / Q000032:analysis*
chemical_list: D000199:Actins; D003285:Contractile Proteins; D008840:Microfilament Proteins; D051304:Profilins; D011506:Proteins; C024782:profilactin; D012967:Sodium Dodecyl Sulfate