Molecular cloning and characterization of a complete DNA copy of potato spindle tuber viroid RNA.
Double-stranded cDNA has been synthesized from RNA of a severe strain of potato spindle tuber viroid using a synthetic oligodeoxyribonucleotide as a primer. Upon cloning in bacteriophage M13mp9, two recombinant phages were selected to construct a pBR322-derived plasmid containing a complete viroid DNA copy. Elucidation of the nucleotide sequence revealed four differences with the previously established sequence of another PSTV strain, three of which were base exchanges and one a deletion.
D001483:Base Sequence; D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics; D004247:DNA / Q000378:metabolism*; D004274:DNA, Recombinant / Q000378:metabolism; D009838:Oligodeoxyribonucleotides; D010942:Plant Viruses / Q000235:genetics*; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics*; D014772:Viroids / Q000235:genetics*
D004274:DNA, Recombinant; D009838:Oligodeoxyribonucleotides; D012367:RNA, Viral; D004247:DNA