Stereospecific radioimmunoassays for l-ephedrine and d-ephedrine in human plasma.
Haptens were prepared by the reaction of d-ephedrine or l-ephedrine with methyl acrylate and subsequent alkaline hydrolysis of the methyl ester groups. The haptens were coupled to bovine serum albumin by a mixed anhydride method, and the resulting drug-protein conjugates were used to immunize rabbits. Antisera raised to these conjugates were highly stereospecific. Neither antiserum cross-reacted with the optical antipode of its substrate nor with racemic pseudoephedrine. Separate radioimmunoassays (RIAs), developed for d-ephedrine and l-ephedrine, were used to measure the concentrations of the enantiomers of ephedrine in the blood of two volunteers dosed with racemic ephedrine. The RIAs were validated by comparing the sum of the concentrations of the enantiomers, determined by RIA, with total ephedrine concentrations determined by a nonstereoselective GLC-ECD method.
D000818:Animals; D000918:Antibody Specificity; D003429:Cross Reactions; D004809:Ephedrine / Q000097:blood*; D005260:Female; D006801:Humans; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D013237:Stereoisomerism
D004809:Ephedrine