Effect of porcine relaxin and estradiol-17 beta on the incorporation of tritiated thymidine by fibroblasts isolated from guinea pig uteri.
Fibroblasts isolated from the uteri of 5 guinea pigs were cultured in vitro to determine the effect of relaxin and estradiol. Both relaxin and estradiol increased thymidine uptake by fibroblasts. Thymidine incorporation with 1.5 micrograms/ml relaxin and no estradiol was 107% of control while 600 pg/ml estradiol and no relaxin gave 112% of control. Thymidine incorporation in the presence of 1.5 micrograms/ml relaxin and 600 pg/ml estradiol was 128% of control. This indicated a significant interaction between relaxin and estradiol.
D000818:Animals; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006168:Guinea Pigs; D007700:Kinetics; D012065:Relaxin / Q000494:pharmacology*; D013552:Swine; D013936:Thymidine / Q000378:metabolism; D014599:Uterus / Q000378:metabolism*
D004958:Estradiol; D012065:Relaxin; D013936:Thymidine