title: Effect of acclimation temperature on oxygen transport in the blood of the carp, Cyprinus carpio.
abstract: The temperature dependence of the O2 equilibrium in whole blood was measured in carp acclimated for more than 3 weeks at 10 degrees C or 20 degrees C water temperature. O2 combining curves were obtained for the same samples at 10 degrees C or 20 degrees C using blood from the 10 degrees C or 20 degrees C acclimated fish (group A) or only at the acclimation temperature (group B). Whereas in group B the P50 was about the same at both temperatures, in group A P50 was higher at 20 degrees C than at 10 degrees C, yielding an apparent heat of oxygenation delta H = -9.9 kcal/mol at pH = 8.0. The CO2 Bohr effect in group A was delta log P50/delta pH = -0.93 at 20 degrees C and -1.17 at 10 degrees C, whereas in group B no temperature effect was seen (-0.98 and -0.97). The acclimation temperature had no effect on the electrophoretic Hb pattern. As expected, the in vivo pH changed inversely with temperature from 8.06 at 10 degrees C to 7.73 at 20 degrees C, enhancing the temperature-induced shift in P50. Acclimation reverses partly the changes in O2 affinity, thereby improving the uptake of oxygen in the gills.
mesh_terms: D000064:Acclimatization; D000818:Animals; D002347:Carps / Q000502:physiology*; D003530:Cyprinidae / Q000502:physiology*; D006454:Hemoglobins / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010100:Oxygen / Q000097:blood*; D013696:Temperature
chemical_list: D006454:Hemoglobins; D010100:Oxygen