Determination of platelet factor 3 by thrombin generation using a micro-coagulation assay.
Platelet factor 3 activity is usually determined by the Stypven method in which factor Xa generation is measured indirectly. The determination is dependent on factors I, II, V, X in the test sample. A coagulation assay for platelet factor 3, which measures the thrombin generation promoted by platelet factor 3, is presented. The test depends on platelet factor 3 only, because factors II, Xa, V, fibrinogen and Ca++ are added in excess. The usability of the new method is shown by platelet factor 3 determinations in patients with various platelet diseases, after ingestion of aspirin and in samples of platelet concentrates before and after storage.
D001241:Aspirin / Q000494:pharmacology; D001779:Blood Coagulation Factors / Q000032:analysis*; D001780:Blood Coagulation Tests / Q000379:methods*; D001791:Blood Platelet Disorders / Q000097:blood / Q000175:diagnosis; D001792:Blood Platelets / Q000378:metabolism; D006801:Humans; D010974:Platelet Aggregation / Q000187:drug effects; D010977:Platelet Factor 3 / Q000032:analysis*; D013917:Thrombin / Q000096:biosynthesis*; D013997:Time Factors
D001779:Blood Coagulation Factors; D010977:Platelet Factor 3; D013917:Thrombin; D001241:Aspirin