Geometry of phase-separated domains in phospholipid bilayers by diffraction-contrast electron microscopy.
The sizes and shapes of solidus (gel) phase domains in the hydrated molecular bilayers of dilauroylphosphatidylcholine/dipalmitoylphasphatidylcholine (DLPC/DPPC) (1:1) and phosphatidylserine (PS)/DPPC (1:2) are visualized directly by low dose diffraction-contrast electron microscopy. The temperature and humidity of the bilayers are controlled by an environmental chamber set in an electron microscope. The contrast between crystalline domains is enhanced by electron optical filtering of the diffraction patterns of the bilayers. The domains are seen as a patchwork in the plane of the bilayer, with an average width of 0.2-0.5 micrometer. The percentage of solidus area measured from diffraction-contrast micrographs at various temperatures agrees in general with those depicted by known phase diagrams. The shape and size of the domains resemble those seen by freeze-fracture in multilamellar vesicles. Temperature-related changes in domain size and in phase boundary per unit area are more pronounced in the less miscible DLPC/DPPC mixture. No significant change in these geometric parameters with temperature is found in the PS/DPPC mixture. Mapping domains by their molecular diffraction signals not only verifies the existance of areas of different molecular packing during phase separation but also provides a quantitative measurement of structural boundaries and defects in lipid bilayers.
D055592:Biophysical Phenomena; D001703:Biophysics; D008081:Liposomes; D008854:Microscopy, Electron / Q000379:methods*; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D011663:Pulmonary Surfactants
D008081:Liposomes; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D011663:Pulmonary Surfactants; C015153:1,2-dilauroylphosphatidylcholine