title: Thin-layer chromatographic determination of major metamizole metabolites in serum and urine.
abstract: Thin-layer chromatographic (TLC) methods were developed for pharmacokinetic studies of major metamizole metabolites in serum and urine. These methods proved practicable, selective, accurate and sensitive with detection limits as follows: 4-methylaminoantipyrine 0.6 micrograms/ml serum and 2.0 micrograms/ml urine; 4-aminoantipyrine 0.16 micrograms/ml serum and 1.0 micrograms/ml urine; 4-acetylaminoantipyrine 0.14 micrograms/ml serum and 0.6 micrograms/ml urine; 4-formylaminoantipyrine 0.12 micrograms/ml serum and 1.0 micrograms/ml urine. Samples of 250 microliter suffice for TLC analysis. Following chromatographic separation, detection is performed in the ultraviolet range at 265 nm. Serum and urine levels were determined following a single oral dose of 1 g of metamizole sodium to eight volunteers. These results were compared with those following an accidental overdose of 49 g.
mesh_terms: D000293:Adolescent; D000632:Aminopyrine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D000675:Ampyrone / Q000031:analogs & derivatives / Q000302:isolation & purification; D002855:Chromatography, Thin Layer; D004177:Dipyrone / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000378:metabolism* / Q000506:poisoning; D005260:Female; D006207:Half-Life; D006801:Humans; D007700:Kinetics; D047069:Pyrazolones
chemical_list: D047069:Pyrazolones; D000632:Aminopyrine; D000675:Ampyrone; C011256:4-formylaminoantipyrine; D004177:Dipyrone; C010403:N-acetylaminoantipyrine; C008667:noramidopyrine