title: Two distinct classes of polyuronide from the cell walls of a dimorphic fungus, Mucor rouxii.
abstract: Polyuronides were extracted from purified yeast and mycelial walls of Mucor rouxii by sequential treatments with lithium chloride and potassium hydroxide and were fractionated by ion-exchange chromatography on DEAE-Sephadex. Two polymers (I and II) of different acidity were found in both wall types. Polymer I contained D-glucuronic acid, L-fucose, D-mannose, and much smaller amounts of D-galactose. Yeast and mycelial polymer I had similar uronic acid contents but differed in their neutral sugar compositions and molecular weights. Polymer II from both cell types contained largely D-glucuronic acid and had similar molecular weights. On partial acid hydrolysis, both polymers I and II gave rise to insoluble glucuronans which appeared to be homopolymeric. One-third of the total uronosyl residues of polymer I, and almost all of the uronosyl residues of polymer II, were present in homopolymeric segments. However, homopolymers derived from polymers I and II may not be identical.
mesh_terms: D002473:Cell Wall / Q000032:analysis; D002852:Chromatography, Ion Exchange; D005643:Fucose / Q000032:analysis; D005965:Glucuronates / Q000032:analysis; D020723:Glucuronic Acid; D006868:Hydrolysis; D008358:Mannose / Q000032:analysis; D008970:Molecular Weight; D009089:Mucor / Q000032:analysis*; D011108:Polymers / Q000032:analysis; D014574:Uronic Acids / Q000032:analysis*
chemical_list: D005965:Glucuronates; D011108:Polymers; D014574:Uronic Acids; C018402:traumacel; D005643:Fucose; D020723:Glucuronic Acid; D008358:Mannose