[Investigation of model and biological membranes by probes emitting annihilated delayed fluorescence].
Registration of the annihilated delayed fluorescence kinetics of anthracene, 1,2-benzanthracene, 1,2,5,6-dibenzanthracene, 7,12-dimethylbenzanthracene, pyrene, 1,2-benzpyrene, 3,4-benzpyrene, perylene, and other aromatic compounds under laser excitation permitted investigation of the quenching of excited triplet molecules by hydrophobic quenchers (ferrocene, nitroxide radicals). A long life-time of the triplet excited states (10(-4)-10(-5) s) makes it possible to investigate the probe-quencher interactions in liposome and natural membranes at the probe concentration of 10(-6)-10(-7) M and the ratio of quencher/lipid = = 10(-4). The method enables to investigate localization of the enzyme active centre (hemes, non-heme iron, flavine) and lipid matrix organization in the lipid-protein complexes.
D005456:Fluorescent Dyes; D008081:Liposomes; D008433:Mathematics; D008566:Membranes / Q000648:ultrastructure*; D008954:Models, Biological; D013329:Structure-Activity Relationship
D005456:Fluorescent Dyes; D008081:Liposomes
Primenenie zondov, ispuskaiushchikh annigiliatsionnuiu zamedlennuiu fluorestsentsiiu, dlia issledovaniia model'nykh i biologicheskikh membran.