title: Free energy changes in ribonuclease A denaturation. Effect of urea, guanidine hydrochloride, and lithium salts.
abstract: The unfolding of ribonuclease A by urea, guanidine hydrochloride, lithium perchlorate, lithium chloride, and lithium bromide has been followed by circular dichroic and difference spectral measurements. All three abnormal tyrosyl residues are normalized in urea and guanidine hydrochloride (delta epsilon 287 = -2700), only two are normalized in lithium bromide and lithium perchlorate (delta epsilon 287 = -1700), and only one is exposed in lithium chloride solutions (delta epsilon 287 = -700). The Gibbs energies are 4.7 +/- 0.1 kcal mol-1 for urea- and guanidine hydrochloride-denaturation, 3.8 +/- 0.2 kcal mol-1 for lithium perchlorate-denaturation, and 12.7 +/- 0.2 kcal mol-1 for lithium chloride- and lithium bromide-denaturation of ribonuclease A. The latter results suggest that the mechanism of the unfolding process in urea and guanidine hydrochloride is quite different from that in lithium salts.
mesh_terms: D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D004722:Endoribonucleases / Q000378:metabolism*; D019791:Guanidine; D006146:Guanidines; D008094:Lithium; D008433:Mathematics; D010179:Pancreas / Q000201:enzymology; D011489:Protein Denaturation; D012259:Ribonuclease, Pancreatic; D014508:Urea
chemical_list: D006146:Guanidines; D014508:Urea; D008094:Lithium; D004722:Endoribonucleases; D012259:Ribonuclease, Pancreatic; D019791:Guanidine