Antitumor activity of polysaccharide TC-13 extracted from rare actinomycetes.
The antitumor activities of mycelia and a hot-water extract of mycelia of rare actinomycetes were examined by three different antitumor assays in ddY mice with Ehrlich solid tumor. A new antitumor polysaccharide, TC-13, was prepared from the hot-water extract of Microellobosporia grisea by precipitation with cetylpyridinium chlorideborate complex and gel filtration. TC-13 was mainly composed of glucose and mannose with peptideglycan. Its molecular weight was determined to be 2-30 X 10(4) by gel filtration. Comparative studies suggested that the antitumor activity of TC-13 was equal to, or stronger than those of other antitumor agents (e.g., lentinan, PS-K, OK432 and yeast mannan). Screening methods for antitumor activity of actinomycetes were also discussed.
D000192:Actinomycetales / Q000032:analysis*; D000818:Animals; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification / Q000627:therapeutic use*; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy*; D004353:Drug Evaluation, Preclinical; D006020:Glycopeptides; D006023:Glycoproteins / Q000302:isolation & purification / Q000627:therapeutic use; D008297:Male; D051379:Mice
D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D006020:Glycopeptides; D006023:Glycoproteins; C035790:TC 13