title: Regulatory properties of 14-day embryo and adult hen skeletal muscle AMP deaminase.
abstract: Chromatography on phosphocellulose column revealed changes in the elution profile of 14 day-old chicken embryo and adult hen skeletal muscle AMP deaminase. In the presence of 5 mM potassium the enzyme from embryo muscle exhibited a sigmoid-shaped plot of the reaction rate versus substrate concentration. The increase of KCl concentration up to 100 mM diminished distinctly sigmoidicity of the plot. Micromolar concentrations of ADP or ATP activated, whereas GTP at the same concentrations inhibited the embryo and hen skeletal muscle AMP deaminase while 5 mM KCl was present in the incubation medium. 100 mM potassium concentration diminished the effect of ADP and ATP but not of GTP. Palmitoyl-CoA inhibited strongly the embryo skeletal muscle adenylate deaminase but had no effect on the activity of the hen enzyme. Alanine inhibited only the adult hen enzyme. The embryo and hen AMP deaminase differed also in the specificity to adenylate analogues and exhibited a different dAMP/AMP ratio. The data presented indicate that kinetic and regulatory properties of the two developmental forms of AMP deaminase are different.
mesh_terms: D000659:AMP Deaminase / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D006160:Guanosine Triphosphate / Q000378:metabolism; D009132:Muscles / Q000196:embryology / Q000201:enzymology*; D009709:Nucleotide Deaminases / Q000378:metabolism*; D010171:Palmitoyl Coenzyme A / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011487:Protein Conformation
chemical_list: D010171:Palmitoyl Coenzyme A; D000244:Adenosine Diphosphate; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D009709:Nucleotide Deaminases; D000659:AMP Deaminase; D011188:Potassium