Influence of membrane (M) protein on influenza A virus virion transcriptase activity in vitro and its susceptibility to rimantadine.
The transcriptase activity of influenza A virus ribonucleoproteins was inhibited by 42 to 49% in vitro in the presence of membrane (M) protein. The addition of M protein to the system of ribonucleoprotein preparations isolated from rimantadine-sensitive or rimantadine-resistant influenza virus strains, as well as the addition of M protein isolated from a sensitive strain, in the presence of rimantadine further inhibited the transcriptase activity of such complexes by approximately 40%. In the system containing the same ribonucleoprotein preparations, but with M protein isolated from a rimantadine-resistant influenza virus strain, the transcriptase activity was not sensitive to rimantadine. The data show that M protein can influence the activity of influenza A virus virion transcriptase and that the susceptibility of influenza virus to rimantadine may be due to the peculiarities of M protein.
D000218:Adamantane / Q000031:analogs & derivatives*; D000818:Animals; D002642:Chick Embryo; D004352:Drug Resistance, Microbial; D009980:Influenza A virus / Q000378:metabolism*; D008565:Membrane Proteins / Q000494:pharmacology*; D012316:RNA Nucleotidyltransferases / Q000037:antagonists & inhibitors*; D012367:RNA, Viral / Q000096:biosynthesis; D012324:RNA-Dependent RNA Polymerase / Q000037:antagonists & inhibitors*; D012299:Rimantadine / Q000494:pharmacology*
D008565:Membrane Proteins; D012367:RNA, Viral; D012299:Rimantadine; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase; D000218:Adamantane