Simple procedure for the isolation of mouse interferon-beta.
We report a simple procedure for the isolation of a mouse interferon beta (beta IFN) from Ehrlich ascites tumor cells infected with Newcastle disease virus. The procedure consists of four chromatographic steps (two on controlled pore glass, one on CM-Sephadex, and one on phosphocellulose) with stepwise elution. The product purified is a 35-40 kD beta IFN with a specific activity of 10(9) units/mg protein.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002845:Chromatography / Q000379:methods*; D007370:Interferon Type I / Q000302:isolation & purification*; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism
D007370:Interferon Type I