title: [Testosterone concentrations in human biological fluids following various routes of administration].
abstract: The antiserum against testosterone (T) was obtained and its titer-dependent specificity was studied during the rabbit immunization. The radioimmunoassay for T determination in the human saliva, blood serum and urine was developed. The sensitivity is 6 pg, the variation coefficient within the experiment is 5.3 and between experiments is 10.5%, the standard confirmation is 95 to 100% and dihydrotestosterone cross reaction is 62%. The T content in human biological liquids was studied after various routes of its administration. The highest steroid concentration in the blood is seen 0.5 and 2 hours after the oral and intramuscular administration, exceeding the initial level 2-3 times. The highest T concentrations in the saliva and urine are observed 0.5 and 2, 2 and 24 hours after the oral and intramuscular use, respectively, the levels being 40 to 90 times (oral administration) and 2-3 fold (intramuscular injections) highest versus the initial ones. The changes in the saliva T content indicate the T level in the blood. It was shown that the free and total T levels in sportswomen urine are markedly higher in comparison with those of the females, not going in for sport. The T content in the sportsmen urine is within the limits, characteristic for healthy males.
mesh_terms: D000284:Administration, Oral; D000818:Animals; D005260:Female; D006801:Humans; D007273:Injections, Intramuscular; D008297:Male; D011817:Rabbits; D011863:Radioimmunoassay; D012463:Saliva / Q000032:analysis*; D013178:Sports Medicine; D013739:Testosterone / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D013739:Testosterone
vernacular_title: Soderzhanie testosterona v biologicheskikh zhidkostiakh cheloveka pri razlichnykh sposobakh vvedeniia v organizm.