Estrogen receptor-mediated cytotoxicity using iodine-125.
Auger effects from 125I decay are singularly damaging if localized in DNA as the thymidine analogue 125I-iododeoxyuridine (125IUdR). Recent experience with steroid sex hormones extends these observations by demonstrating cytotoxicity in sites other than the DNA backbone. We have compared the cytotoxicity in human MCF-7 breast cancer cells of 125IUdR, 125I-iodotamoxifen, a nonsteroidal antiestrogen that is translocated from the cytoplasm to the nucleus of receptor containing cells, and 125I-iodoantipyrine, a biological indicator of the body water space. Cytotoxicity is critically dependent upon subcellular localization.
D000983:Antipyrine / Q000031:analogs & derivatives / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism / Q000532:radiotherapy; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects*; D006801:Humans; D007065:Idoxuridine / Q000378:metabolism; D007457:Iodine Radioisotopes / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism*; D013629:Tamoxifen / Q000031:analogs & derivatives / Q000378:metabolism
D007457:Iodine Radioisotopes; D011960:Receptors, Estrogen; D013629:Tamoxifen; C035179:iodotamoxifen; D007065:Idoxuridine; D000983:Antipyrine; C008135:iodoantipyrine