title: [Isoelectrofocusing in the study of the heterogeneity of immunoglobulins G in man with or without proliferative symptomatology].
abstract: In the present investigation, the immunoglobulins G of human sera were studied after isoelectrofocusing using polyacrylamide flat bed gel. The heterogeneity of immunoglobulins was demonstrated in normal serum as well as in pathological situations. Isoelectrofocusing study was performed using the total serum or the immunoglobulin's preparations obtained after absorption and elution on staphylococcus protein A. These methods respect the integrity of immunoglobulins and allow the study of native immunoglobulins. Furthermore it was demonstrated the micro-heterogeneity of immunoglobulins (antibody) induced by antitetanic vaccination.
mesh_terms: D004406:Dysgammaglobulinemia / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis / Q000235:genetics*; D007525:Isoelectric Focusing
chemical_list: D007075:Immunoglobulin M
vernacular_title: Apport de l'isoélectrofocalisation à l'étude de l'hétérogénéité des immunoglobulines G chez l'homme avec ou sans symptomatologie proliférative.