Fibronectin remains in the cytoskeletal preparations of cultured human fibroblasts.
Cytoskeleton models were produced by treating cultured human embryonal fibroblasts with Triton X-100 and actomyosin dissociating buffers. Morphological studies indicated that in addition to nuclear residues and intermediate filaments, both plasma membrane remnants and substratum-associated, pericellular material remained on the culture substratum. By immunochemical analysis the extracellular material was identified as fibronectin. The results suggest that fibronectin is associated with the nucleus-anchoring cytoskeleton of cultured human fibroblasts.
D000199:Actins / Q000378:metabolism; D002448:Cell Adhesion; D003599:Cytoskeleton / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure*; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D009124:Muscle Proteins / Q000378:metabolism; D014746:Vimentin
D000199:Actins; D005353:Fibronectins; D009124:Muscle Proteins; D014746:Vimentin