Modulation by vasoactive intestinal peptide of serotonin receptors in the dorsal hippocampus of the rat brain: an autoradiographic study.
Brain sections of 32 microns from the hippocampus were incubated with tritiated serotonin (5-HT) in the presence or absence of 10(-7) M vasoactive intestinal peptide (VIP). Sections were run for biochemical Scatchard analysis or quantitative autoradiography by means of LKB 3H-Ultrofilm. On sections from dorsal hippocampus, VIP increases the amount of 5-HT1 receptors and decreases the affinity for the ligand. Densities measurements show that this effect is located in a discrete area of the hippocampus, the dorsal subiculum. The present data suggest that some of the neurotransmitter effects of 5-HT in the central nervous system can be modulated by VIP.
D000818:Animals; D001345:Autoradiography; D005768:Gastrointestinal Hormones / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011985:Receptors, Serotonin / Q000187:drug effects* / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D005768:Gastrointestinal Hormones; D011985:Receptors, Serotonin; D012701:Serotonin; D014660:Vasoactive Intestinal Peptide