Assignment of superoxide dismutase (SOD-1) gene to chromosome No. 9 of domestic pig.
Pig--mouse somatic cell hybrids were obtained by fusing pig lymphocytes with mouse cells of the RAG (HPTR-) line using polyethylene glycol. The expression of pig superoxide dismutase (SOD-1) activity in 11 hybrid clones as well as in the parental cells was investigated. Seven hybrid clones positive for the expression of pig SOD-1 displayed the corresponding parental RAG and pig bands of SOD-1 plus a heteropolymeric band of intermediate electrophoretic mobility. The positive and negative expression of pig SOD-1 was concordant with the retention and loss of pig chromosome No. 9, suggesting that the gene for pig SOD-1 is located on the chromosome No. 9 of the domestic pig.
D000818:Animals; D002874:Chromosome Mapping; D005796:Genes; D006822:Hybrid Cells / Q000201:enzymology; D051379:Mice; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000235:genetics*; D013552:Swine / Q000235:genetics*
D013482:Superoxide Dismutase