title: Effect of vitamin D on the intestinal absorption of 203Pb and 47Ca in chicks.
abstract: The transfer of 203Pb and/or 47Ca across the intestinal epithelium of the chick was investigated, with emphasis given to the functional role of cholecalciferol (vitamin D-3). 203Pb, after introduction in the intestinal lumen, is rapidly accumulated by the intestinal tissue, and only a fraction of 203Pb is translocated parenterally (absorbed). Cholecalciferol did not significantly affect the accumulation of 203Pb by intestinal tissue but did accelerate 203Pb movement across the basal-lateral membrane. In contrast, cholecalciferol both decreased 47Ca tissue levels and increased 47Ca absorption. In rachitic chicks, the rate of absorption of 203Pb was greater in the distal than in the proximal segments of the intestine; after cholecalciferol repletion, the degree of absorption in al segments was similar, indicting the order of cholecalciferol effectiveness as duodenum greater than or equal to jejunum greater than ileum. An acute dose of 1,25(OH)2D3 to rachitic chicks also enhanced both 203Pb and 47Ca absorption, but the time course and pattern of absorption of these metal cations differed. The time at which the absorption of 203Pb peaked and returned to base-line occurred sooner than for 47Ca. Also the back-flux (blood leads to intestinal lumen) of 47Ca was enhanced by cholecalciferol, whereas no effect on the back-flux of 203Pb was noted. These studies show that cholecalciferol and 1,25(OH)2D3 affects both the 203Pb and 47Ca absorptive processes, but the nature of these responses are not identical, suggesting differences in the transport path or the macromolecular interactions of these metal ions during the course of absorption, or both.
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002117:Calcitriol / Q000494:pharmacology; D002132:Calcium Radioisotopes / Q000378:metabolism*; D002645:Chickens; D004386:Duodenum / Q000378:metabolism; D007408:Intestinal Absorption / Q000187:drug effects*; D007854:Lead / Q000378:metabolism*; D011868:Radioisotopes / Q000378:metabolism*; D012279:Rickets / Q000378:metabolism; D014807:Vitamin D / Q000494:pharmacology*
chemical_list: D002132:Calcium Radioisotopes; D011868:Radioisotopes; D014807:Vitamin D; D007854:Lead; D002117:Calcitriol