title: Expression of the albumin gene in rat hepatoma cells and their dedifferentiated variants.
abstract: Rat hepatoma clones whose cells do and do not produce albumin, as well as somatic hybrid between the two types of cells, have been examined for albumin mRNA. A direct proportionality between the rate of albumin production and the concentration of albumin mRNA sequences was found for all albumin-producing hepatoma and hybrid clones, indicating that rate of synthesis of the protein is determined by the concentration of its mRNA. Albumin-negative dedifferentiated variant and somatic hybrid cells contain fewer than one to five molecules of albumin mRNA per cell; the block in expression of the gene appears to be at the same (probably transcriptional) level in variants and their somatic hybrids.
mesh_terms: D000418:Albumins / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D002454:Cell Differentiation; D002999:Clone Cells; D004247:DNA; D005786:Gene Expression Regulation; D006822:Hybrid Cells / Q000378:metabolism; D007700:Kinetics; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D014158:Transcription, Genetic
chemical_list: D000418:Albumins; D012333:RNA, Messenger; D004247:DNA