[Adaptation of ecthyma contagiosum virus to cell culture].
Series of attempts were made to adapt the Phylaxia vaccinal strain (Hungary) to various cell cultures in vitro. The work with twenty-one successive passages of the virus in a culture of lamb testis resulted in the production of an adapted strain that could produce a characteristic cytopathic effect at the 24th--48th hour of the infection, reaching a titer of 10(4.5) ID/cm3. Cytologic and electron-microscope investigations confirmed the fact that this cell strain belonged to the Parapoxvirus genus. The experimental infection of 4-5-month-old lambs showed that the virus strain retained its capacity to cause typical clinical signs of infectious ectyma. It could be used in the production of a cell-cultural vaccine.
D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D008297:Male; D008854:Microscopy, Electron; D009923:Orf virus / Q000254:growth & development / Q000472:pathogenicity / Q000648:ultrastructure*; D011212:Poxviridae / Q000648:ultrastructure*; D012756:Sheep; D013737:Testis; D014776:Virus Cultivation
Opiti na adaptirane na virusa na zaraznata ektima vurkhu kletuchni kulturi.