Analysis of corticosteroids in human adrenal tissue by high pressure liquid chromatography.
High pressure liquid chromatography (HPLC) was demonstrated to be a good tool for the separation, identification and quantitation of corticosteroids (CS) extracted from homogenized tissue of adrenal glands and adrenal tumors in patients with hypercorticism. A chromatographic system consisting of Sorbax-SIL and Sorbax-CN columns, organic solvent extraction and a UV detector was used to analyze both more polar and less polar corticosteroids in the adrenal tissue.
D000305:Adrenal Cortex Hormones / Q000032:analysis*; D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000311:Adrenal Glands / Q000032:analysis*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003480:Cushing Syndrome / Q000378:metabolism; D006801:Humans; D006929:Hyperaldosteronism / Q000378:metabolism; D007211:Indoles / Q000652:urine; D007737:Kynurenine / Q000378:metabolism; D014364:Tryptophan / Q000097:blood
D000305:Adrenal Cortex Hormones; D000900:Anti-Bacterial Agents; D007211:Indoles; D007737:Kynurenine; D014364:Tryptophan