Uptake of 6-fluoro-5-hydroxytryptamine and 4,6-difluoro-5-hydroxytryptamine into releasable and non-releasable compartments of human platelets.
1 The ring-fluorinated compounds, 6-fluoro-5-hydroxytryptamine and 4,6-difluoro-5-hydroxytryptamine, are sterically similar to 5-hydroxytryptamine (5-HT) but have pKaS for their 5-hydroxyl groups (9.7 and 8.0 respectively) which are lower than that of 5-HT. 2 The rates at which [3H]-5-HT, [3H]-6-fluoro-5-HT, and [3H]-4,6-difluoro-5-HT entered the releasable (vesicular) and non-releasable compartments of washed human platelets during a 1 min incubation period at 37 degrees C were similar. These portions of the uptake process therefore appears to be relatively independent of the pKa of the 5-hydroxyl group. 3 The [3H]-4,6-difluoro-5-HT was unique since material accumulated in the non-releasable compartment during uptake did not migrate into the vesicular compartment under appropriate incubation conditions. 4 The data suggest that the zwitterionic form of 5-HT is not the transported species, but that the electronic configuration about the 5-hydroxyl group may be critical for translocation from a non-releasable to a releasable compartment.
D001792:Blood Platelets / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D012701:Serotonin / Q000031:analogs & derivatives* / Q000097:blood; D013329:Structure-Activity Relationship
D012701:Serotonin; C032744:6-fluoroserotonin; C016378:4,6-difluoroserotonin