DNA-binding proteins in frog virus 3-infected cells.
At least 12 virus-induced DNA-binding proteins with mol. wt. ranging from 14 X 10(3) have been isolated from frog virus (FV 3)-infected fathead minnow cells by DNA affinity chromatography. Two enzymic activities, DNA-dependent DNA polymerase and endodeoxyribonuclease, were present in the DNA-binding proteins; these enzymic activities were similar to those induced by FV 3 in infected cells. A single species of DNA-binding proteins with a mol. wt of 36 000 had very high affinity for single-stranded DNA, but a low one for double-stranded DNA. Proteins with such characteristic affinity for single-stranded DNA destabilize the DNA helix and are essential for viral DNA replication. Thus, the 36 000 mol. wt. DNA-binding protein is a candidate for such a role in FV 3 DNA replication.
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification*; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004268:DNA-Binding Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D007497:Iridoviridae / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification*
D002352:Carrier Proteins; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D014764:Viral Proteins; D004247:DNA