Preparation of the alkali and P light chains of chicken gizzard myosin. Amino acid sequence of the alkali light chain.
1. A simple method is described for the purification of the alkali and P light chains from chicken gizzard myosin. 2. The sequence of the alkali light chain has been unequivocally determined, except for the N-terminal dipeptide, by using the tryptic and CNBr peptides. 3. No evidence was obtained for any specific high-affinity Ca2+-binding sites on the alkali light chain. 4. Detailed evidence on which the sequence is based has been deposited as Supplementary Publication SUP 50120 (14 pages) at the British Library Lending Division, Boston Spa, Wetherby, West Yorkshire LS23 7QB, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1983) 209, 5.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens; D003488:Cyanogen Bromide; D005895:Gizzard, Avian / Q000032:analysis; D008970:Molecular Weight; D009218:Myosins / Q000302:isolation & purification; D010455:Peptides / Q000032:analysis; D014357:Trypsin
D000596:Amino Acids; D010455:Peptides; D014357:Trypsin; D009218:Myosins; D003488:Cyanogen Bromide