Immunogenicity of N-(2-hydroxypropyl)-methacrylamide copolymers--potential hapten or drug carriers.
After repeated i.p. immunizations of mice with 10 micrograms of homopolymer poly (HPMA) no antibodies were detected by the ELISA test. Immunization with copolymer P-Acap-Leu-HMDA leads to a weak antibody response, while immunization with a copolymer with some side chains modified with ARS or FITC groups (P-Acap-Leu-HMDA-ARS or P-Acap-Leu-HMDA-FITC) leads to a significant antibody response detectable by PFC, ELISA and haemagglutination tests. Most of these antibodies are aimed against the modifying haptenic group, a smaller amount against side oligopeptide sequences of the carrier. Intensity of the antibody response depends on: 1) the antigen dose--the optimal dose was 10 micrograms: both the higher (100 micrograms) and the lower doses (1 and 0.1 micrograms) induced considerably lower antibody responses; 2) molar mass of the immunizing fractions--fractions of high molar mass induced up to five times higher responses than those of a low molar mass; 3) the bound haptenic group--the ARS-copolymers induced ten times lower response than the FITC-copolymers. We detected no difference between capacities of the H-2a, H-2b and H-2d haplotypes to react with anti-ARS antibodies after immunization with P-Acap-Leu-HMDA-ARS.
D000178:Acrylamides / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D006183:H-2 Antigens / Q000235:genetics; D006241:Haptens / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology; D008815:Mice, Inbred Strains / Q000276:immunology; D014677:Pharmaceutical Vehicles / Q000276:immunology; D011109:Polymethacrylic Acids / Q000276:immunology*
D000178:Acrylamides; D000906:Antibodies; D006183:H-2 Antigens; D006241:Haptens; D014677:Pharmaceutical Vehicles; D011109:Polymethacrylic Acids; C032976:N-(2-hydroxypropyl)methacrylamide