Detection of a Mr 24,000 estrogen-regulated protein in human breast cancer by monoclonal antibodies.
Monoclonal antibodies have been prepared that can specifically detect an estrogen-regulated protein with a molecular weight of 24,000 in the MCF-7 human breast tumor cell line and in human breast tumor biopsies. These antibodies are of the immunoglobulin G1 subclass, exhibit a high affinity for the Mr 24,000 protein, and recognize an antigenic site that is stable to sodium dodecyl sulfate gel electrophoresis and electrophoretic transfer techniques. These monoclonal antibodies have been used to confirm estrogen regulation of the Mr 24,000 protein in MCF-7 cells and to detect Mr 24,000 protein in certain human breast tumors.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000936:Antigen-Antibody Complex; D001706:Biopsy; D001943:Breast Neoplasms / Q000032:analysis* / Q000473:pathology; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D018832:Molecular Chaperones; D008970:Molecular Weight; D009256:Nafoxidine / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; C038865:estrogen regulated protein; D009256:Nafoxidine