[Methodological studies of protein metabolism and the bioenergetics of protein retention in growing animals. 2. The results of amino acid analysis after the long-term labelling of chicks with 15NH4-acetate].
The amino acid composition of the proteins in selected body fractions of chickens and the 15N-excess of amino acids isolated from them resulting from a feeding experiment with long-term labelling with 15NH4-acetate supplied orally are given. The amino acid spectra of feathers, breast and leg muscles are characterised by differences in the content of individual amino acids specific for the organs, the composition of the proteins, however, is independent of the protein content of the ration and the age of the animals. The sarcoplasmatic and myofibrillar proteins also have typical amino acid patterns, which--with the exception of the histidine content--are neither influenced by the extraction of the proteins from the breast or leg muscles nor by the energy level of the feeding or the age of the animals. There are no significant differences in the metabolisation of the main protein fraction of the breast and leg muscles. The oral supply of 15N ammonium acetate to broilers predominantly labels the non-essential amino acids so that the derived kinetic data chiefly represent the metabolism of the non-essential amino acids.
D000085:Acetates / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D004044:Dietary Proteins / Q000008:administration & dosage / Q000378:metabolism*; D004734:Energy Metabolism; D005241:Feathers / Q000032:analysis; D008297:Male; D008722:Methods; D009132:Muscles / Q000032:analysis; D009587:Nitrogen Isotopes; D013997:Time Factors
D000085:Acetates; D000596:Amino Acids; D004044:Dietary Proteins; D009587:Nitrogen Isotopes; C018824:ammonium acetate
Methodische Untersuchungen zum Eiweissumsatz und zur Bioenergetik des Eiweissansatzes bei wachsenden Tieren. 2. Mitteilung. Ergebnisse der Aminosäurenanalytik nach Langzeitmarkierung von Küken mit 15NH4-Azetat.