Radioimmunoreactivity and receptor binding activity of specific epididymal proteins of the rat and other species.
Radioimmunoassay (RIA) and radioreceptor assay (RRA) for the 32K Rat Epididymal Protein (REP) have been developed. Washed intact rat epididymal spermatozoa were used as receptors in the RRA. The effects of epididymal and seminal fluids of other species in these assays were studied. The results showed that there is species specificity in the immunoreactivity and receptor binding activity of sperm coating proteins.
D000818:Animals; D001667:Binding, Competitive; D006224:Cricetinae; D004285:Dogs; D004822:Epididymis / Q000378:metabolism*; D006168:Guinea Pigs; D007700:Kinetics; D008297:Male; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011863:Radioimmunoassay / Q000379:methods; D011869:Radioligand Assay; D051381:Rats; D029589:Seminal Plasma Proteins; D013045:Species Specificity; D013094:Spermatozoa / Q000378:metabolism
D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein