Thioguanine resistance, ouabain resistance and sister chromatid exchanges in V79/AP4 Chinese hamster cells treated with potassium dichromate.
The biological activity of potassium dichromate (K2Cr2O7) was assayed in V79/AP4 Chinese hamster cells by measuring two mutational end points, thioguanine (TG) resistance and ouabain (OUA) resistance, and two non-mutational end points, cytotoxicity and sister chromatid exchanges (SCE). By exposing the cells for 1 h to the chemical, all biological end points examined were affected by the treatment in a dose-dependent manner. Moreover the combined use of the two selective systems indicated that chromium induces base-pair substitutions in mammalian cells in culture.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002840:Chromates / Q000494:pharmacology*; D006224:Cricetinae; D003412:Cricetulus; D003434:Crossing Over, Genetic / Q000187:drug effects*; D010042:Ouabain / Q000378:metabolism*; D011192:Potassium Dichromate / Q000494:pharmacology*; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013866:Thioguanine / Q000378:metabolism*
D002840:Chromates; D010042:Ouabain; D013866:Thioguanine; D011192:Potassium Dichromate