Susceptibility of naturally occurring carbapenem antibiotics to renal dehydropeptidase-I. An easy assay using a plate technique based on a novel chromogenic beta-lactamase substrate.
A general screening procedure is described which allows the rapid determination of the susceptibility of naturally occurring carbapenem antibiotics to renal dehydropeptidase-I at the broth level. The procedure is based on the incubation of carbapenem-containing solutions with dehydropeptidase-I and the subsequent assay of residual beta-lactamase-inhibiting/inactivating activity of carbapenems by means of a plate technique using the chromogenic beta-lactamase substrate PADAC.
D000818:Animals; D004150:Dipeptidases / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D013302:Streptomyces; D013552:Swine; D013845:Thienamycins
D013845:Thienamycins; D004150:Dipeptidases; C038937:dipeptidase