Interaction of nystatin with nystatin-resistant Candida tropicalis.
Nystatin-resistant yeast Candida tropicalis was obtained after UV illumination and plating on nystatin-containing media. The mutant contained no ergosterol in the plasma membrane but bound nystatin to a degree similar to that of the wild strain (1.2 vs. 1.5 nmol per mg dry solid). Respiration of the mutant on glucose was reduced by 36% in the presence of 25 microM nystatin. This corresponded to a 25-43% decrease of the uptake of monosaccharides. Transport of amino acids was reduced by nystatin in the mutant by 44-86%, as compared with a 84-95% reduction in the wild strain. The intracellular ATP content was reduced by nystatin equally in the wild strain and in the mutant (by 43 and 47%). Nystatin appears to affect specifically membrane transport processes of nonelectrolytes while both the H+-extruding ATPase and the membrane potential are unaffected.
D001693:Biological Transport, Active / Q000187:drug effects; D002175:Candida / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D004352:Drug Resistance, Microbial; D004734:Energy Metabolism / Q000187:drug effects; D004875:Ergosterol / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism; D009761:Nystatin / Q000378:metabolism / Q000494:pharmacology*
D008563:Membrane Lipids; D009761:Nystatin; D004875:Ergosterol