Studies on liver specific antigens--partial purification, characterization, and immunization.
Seven liver specific antigens, liver protein-I, liver protein-II, liver specific antigen (Espinosa), F-antigen, hepato-renal antigen, liver cell membrane antigen (Ray) and Tamm-Horsfall glycoprotein were partially purified from human liver and urine. Anti-sera against these antigens were raised in rabbits. Anti-sera against liver protein-I, liver protein-II, liver specific antigen (Espinosa) and liver cell membrane antigen (Ray) were absorbed by human serum, kidney, lung and spleen while anti-sera against hepato-renal antigen and Tamm-Horsfall glycoprotein were absorbed by human serum, lung and spleen to remove antibodies against non-specific antigens. There were no cross reactivities among these antibodies.
D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000302:isolation & purification*; D002462:Cell Membrane / Q000276:immunology; D003429:Cross Reactions; D006801:Humans; D007114:Immunization; D007519:Isoantigens / Q000302:isolation & purification*; D008099:Liver / Q000276:immunology*; D008565:Membrane Proteins; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification*; D011817:Rabbits
D000941:Antigens; D007519:Isoantigens; D008565:Membrane Proteins; D011506:Proteins; C030497:liver specific F antigen