title: Synthesis in vitro of rat brown adipose tissue 32 000 Mr protein.
abstract: The synthesis of the mitochondrial inner membrane 32 000 Mr protein from rat brown adipose tissue was examined. Polysomes from the tissue were translated in a reticulocyte lysate protein-synthesizing system and newly-synthesized protein isolated with a monospecific antibody against the 32 000 Mr protein. The newly-synthesized protein had the same relative molecular mass as the mature protein. It was taken up by mitochondria isolated from Chinese hamster ovary cells into a form resistant to trypsin.
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D000918:Antibody Specificity; D002474:Cell-Free System; D006224:Cricetinae; D003412:Cricetulus; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D066298:In Vitro Techniques; D008928:Mitochondria / Q000378:metabolism; D010053:Ovary; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D012156:Reticulocytes / Q000378:metabolism