Growth of normal hemopoietic cells in cultures of bone marrow from leukemic mice.
Bone marrow from mice with spontaneous leukemia was studied in long-term culture in the presence of 10(-7) M hydrocortisone. These conditions resulted in a complete loss of leukemia cells from marrows which initially showed 95% or greater replacement with lymphoblasts. The culture conditions were also found to favor the growth of hemopoietic stem cells and the surviving cultured cells produced sufficient numbers of these cells to protect lethally irradiated (950 rads) syngeneic mice. The irradiated recipients of the cultured marrow did not develop leukemia during the 180 days of observation post-irradiation, indicating the absence of leukemia cells in the inocula. A loss of leukemia cells in vitro occurred in cultures with and without the addition of hydrocortisone to the medium. Hydrocortisone was shown to have a stimulating effect on maintenance of hemopoietic stem cells, granulocyte progenitors and granulopoiesis in these cultures.
D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006854:Hydrocortisone / Q000494:pharmacology; D008223:Lymphoma / Q000473:pathology*; D051379:Mice; D008806:Mice, Inbred AKR; D009374:Neoplasms, Experimental / Q000473:pathology; D013997:Time Factors
D006854:Hydrocortisone