[Candidate selected method for the enzymatic determination of total cholesterol in serum].
An enzymatic method is described for the determination of total cholesterol in serum using a single aqueous reagent which can easily be prepared from commercial substrates and enzymes. The determination is carried out manually, the resulting stable chromogen is measured at a wavelength of 500-550 nm. The cholesterol concentration may be calculated either using a primary cholesterol standard or a constant factor for a given wavelength. The reliability of the method is reported: Data for the imprecision are given on the basis of a survey in 3 laboratories, the accuracy is established by comparison with a definitive and a reference method. Analytical and biological interferances are briefly discussed and results with this enzymatic method are reported concerning the reference values for serum cholesterol.
D002784:Cholesterol / Q000097:blood*; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D012737:Sex Factors; D013053:Spectrophotometry / Q000379:methods / Q000592:standards
D007202:Indicators and Reagents; D002784:Cholesterol
Vorläufig ausgewählte Methode für die enzymatische Bestimmung des Gesamt-Cholesterins im Serum.