title: [Radioimmune determination of steroids--comparison of 2 variants of the methods].
abstract: Various conditions of radioimmunoassay (RIA) technique for estimation of 19-nortestosterone and methandrostenolone were studied. Dilution of antiserum, required for 50% binding of the labelled steroids, depended primarily on the RIA procedure used. When 19-nortestosterone was estimated by means of these two RIA procedures, the final dilution of antiserum was equal to 1:24,000 and 1:475,000, respectively. Characteristics of standard curves, sensitivity and accuracy were similar in these RIA procedures, but the rate of cross-reactions between antiserum and the steroids was different. Both RIA procedures indicated similar values of methandroctenolone and 19-nortestosterone content excreted with urine after oral administration of the steroids.
mesh_terms: D003429:Cross Reactions; D006801:Humans; D008696:Methandrostenolone / Q000032:analysis* / Q000652:urine; D009277:Nandrolone / Q000032:analysis* / Q000652:urine; D011863:Radioimmunoassay / Q000379:methods
chemical_list: D009277:Nandrolone; D008696:Methandrostenolone
vernacular_title: Radioimmunnoe opredelenie steroidov--sravnenie dvukh variantov metoda.