The role of prostaglandins in the modulation of histamine suppression of mitogen responses in atopic and normal subjects.
We have shown that lymphocytes from atopics are more sensitive to histamine-induced suppression of mitogen transformation than are cells from normal subjects. Recent reports have suggested that histamine suppression may act via prostaglandin synthesis. We have investigated this and found that indomethacin partially reversed the histamine suppression seen in atopics, but not that in normals. This effect was seen even when the direct enhancing effect of indomethacin was taken into account. The direct effect of indomethacin on mitogen-induced transformation was similar in both normal and atopic cultures, which suggests that these results are not due to the presence of PG producing cells in atopic cultures, but rather that histamine modulates the function of these cells. It was found that a specific H2 agonist, but not an H1 agonist could partially mimic the effect of histamine this system.
D000293:Adolescent; D000328:Adult; D003208:Concanavalin A / Q000494:pharmacology; D006632:Histamine / Q000276:immunology*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007213:Indomethacin / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects; D008761:Methylhistamines / Q000494:pharmacology; D008875:Middle Aged; D008934:Mitogens / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D011453:Prostaglandins / Q000096:biosynthesis / Q000502:physiology*
D008761:Methylhistamines; D008934:Mitogens; D010835:Phytohemagglutinins; D011453:Prostaglandins; D003208:Concanavalin A; D006632:Histamine; D007213:Indomethacin