Effect of endotoxin and its degradation products on hepatic mixed-function oxidase and heme enzyme systems in mice.
Effect of Salmonella minnesota R595 endotoxin (R595 GL) and its chemical degradation products on hepatic cytochrome P-450, delta-aminolevulinic acid (ALA) synthetase and heme oxygenase activity in mice was examined. The levels of cytochrome P-450 and ALA synthetase activity were significantly decreased following the administration of R595 GL, whereas heme oxygenase activity was markedly increased. The effects of endotoxin were dose-dependent for the range of about 1 to 2 micrograms per mouse. The result of a comparative study using R595 GL and its degradation products suggested that intact lipid A moiety plays an important rôle in the activity of endotoxin. In addition, it was found that the depressant effect of endotoxin on the hepatic mixed-function oxidase system in mice was abrogated by treatment with cortisone.
D000624:5-Aminolevulinate Synthetase / Q000378:metabolism; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D006418:Heme / Q000378:metabolism*; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity*; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D012475:Salmonella; D013997:Time Factors
D008070:Lipopolysaccharides; D006418:Heme; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; D006419:Heme Oxygenase (Decyclizing); D000624:5-Aminolevulinate Synthetase