title: Codon usage and mistranslation. In vivo basal level misreading of the MS2 coat protein message.
abstract: The coat protein of the small RNA virus MS2 shows charge heterogeneity in vivo. In most strains there is a basic satellite of the native protein. We have shown that this basic satellite is greatly diminished or absent in strains with the streptomycin-resistant allele, rpsL, a mutation which leads to increased translational accuracy. Further, the satellite is present in cells where the coat protein is encoded by duplex DNA. Tryptic digests of the satellite show that it contains new lysine-containing peptides which appear to be the same as those found in derivatives of coat protein which have a lysine for asparagine substitution. Sequencing of the NH2-terminal 19 amino acids of the satellite protein shows that the asparagine codon AAU at amino acid 12 is misread approximately 8 times more frequently than the AAC at amino acid 3. We conclude that the satellite species is the result of basal level lysine for asparagine substitution. These substitutions are most likely caused by preferential misreading of AAU codons at a frequency of approximately 5 X 10(-3), 10-fold higher than the average error frequency.
mesh_terms: D000595:Amino Acid Sequence; D002213:Capsid / Q000032:analysis / Q000235:genetics*; D036022:Capsid Proteins; D003062:Codon; D010957:Plasmids; D014176:Protein Biosynthesis; D012328:RNA Viruses / Q000032:analysis; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D014357:Trypsin / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D036022:Capsid Proteins; D003062:Codon; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D014764:Viral Proteins; D014357:Trypsin