Application of the solvent perturbation technique to the study of protein systems by luminescence and light-scattering spectrophotometry.
The solvent perturbation technique was applied to the study of a membrane model consisting of lysozyme and sodium dodecyl sulfate (SDS) micelles, in comparison with erythrocyte ghosts, in aqueous solutions supplemented with dimethyl-sulfoxide. By correlated fluorescence and light-scattering spectrophotometric determinations it was possible to demonstrate a nonmonotonous variation suggesting a phenomenon equivalent to a conformation transition. Such a transition was made evident by the direct action of the cis-[PtCl2(HN3)2]complex on the lysozyme--SDS system at concentrations higher than the critical micelle concentration and at a temperature of 40 degrees C.
D055598:Chemical Phenomena; D002621:Chemistry; D002945:Cisplatin / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004910:Erythrocyte Membrane; D008027:Light; D008567:Membranes, Artificial; D008823:Micelles; D009113:Muramidase; D011487:Protein Conformation; D012542:Scattering, Radiation; D012967:Sodium Dodecyl Sulfate; D013050:Spectrometry, Fluorescence
D008567:Membranes, Artificial; D008823:Micelles; D012967:Sodium Dodecyl Sulfate; D009113:Muramidase; D002945:Cisplatin; D004121:Dimethyl Sulfoxide