[Identification and assay of nonvolatile organic by capillary column gas chromatography].
The assay of organic acids as trimethyl silyl isobutyl compounds by gas chromatography was described. The lyophilization was used instead the extraction by organic reagent of the classical methods. Our method avoids the loss of some hydroxylacids. The identification and the measurement of one organic acid need respectively 3 and 5 hours. Our method is currently used for the metabolic diseases diagnosis.
D002264:Carboxylic Acids / Q000032:analysis* / Q000302:isolation & purification; D002849:Chromatography, Gas / Q000379:methods; D005612:Freeze Drying; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans
D002264:Carboxylic Acids
Identification et dosage des acides organiques non volatils par chromatographie en phase gazeuse sur colonne capillaire.