Human metallothionein genes: molecular cloning and sequence analysis of the mRNA.
From a cDNA clone bank prepared from cadmium-treated HeLa cells, we isolated clones representing mRNAs whose concentration is increased after cadmium induction. Several metallothionein cDNA clones were isolated by cross-hybridization to mouse metallothionein-I cDNA. The nucleotide sequence of one of these clones, containing a nearly full-length cDNA copy of human metallothionein-II mRNA, was determined. The homology between the human and mouse metallothionein sequences is strictly limited to the coding region of the mRNA. Codon usage in metallothionein mRNA is not random. Seventy-nine percent of the codons have G or C residues at the third position, resulting in a GC-rich sequence.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002104:Cadmium / Q000494:pharmacology; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D004247:DNA; D005796:Genes; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008667:Metalloproteins / Q000235:genetics*; D008668:Metallothionein / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*
D003062:Codon; D008667:Metalloproteins; D012333:RNA, Messenger; D002104:Cadmium; D004247:DNA; D008668:Metallothionein