The postribosomal particle of rabbit liver contains protein-synthesis factors and serum albumin mRNA.
As demonstrated by indirect immunoprecipitation and polyacrylamide gel electrophoresis, an 85S particle separated by sucrose density-gradient centrifugation from the postribosomal pellet of rabbit liver, is able to synthesize serum albumin if supplemented with both ribosomal subunits and sources of energy. It is retained on heparin bound to Sepharose 4B, contains translatable mRNA and apparently all protein factors required for translation. This particle may represent a highly organized protein synthesizing machinery, the combination of which with ribosomes results in formation of new protein molecules.
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D012270:Ribosomes / Q000201:enzymology / Q000378:metabolism*; D012709:Serum Albumin / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D012709:Serum Albumin; D000604:Amino Acyl-tRNA Synthetases