title: Synthesis and application of two reagents for the introduction of sulfhydryl groups into proteins.
abstract: Two reagents are described which can be used for the introduction of sulfhydryl groups into proteins. Mercaptopropionylhydrazide modifies specifically periodate-oxidized N termini of proteins, provided that the N-terminal residue is serine or threonine. 3-(Phenyldithio)propionimidate introduces a disulfide bond at lysine residues of proteins. Reduction converts the disulfide into a sulfhydryl group. The imidate compound was found to react with a high specificity with only one lysine residue of ribosomal protein L7/L12.
mesh_terms: D000603:Amino Acids, Sulfur / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000494:pharmacology*; D007202:Indicators and Reagents; D007525:Isoelectric Focusing; D012269:Ribosomal Proteins / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D008625:Tiopronin / Q000031:analogs & derivatives / Q000494:pharmacology*
chemical_list: C038871:3-(phenyldithio)propionimidate; D000603:Amino Acids, Sulfur; D004220:Disulfides; D007202:Indicators and Reagents; D012269:Ribosomal Proteins; D013438:Sulfhydryl Compounds; C038870:mercaptopropionylhydrazide; D008625:Tiopronin