Structure and organization of a mammalian 5 S gene cluster.
We have cloned and characterized a G+C-rich 2.2-kilobase pair Bam HI fragment which contains a single Syrian hamster 5 S rRNA gene. The cloned fragment directs the synthesis in vitro of 5 S RNA catalyzed by RNA polymerase III. Southern hybridization analysis of genomic hamster DNA indicates that the sequences present in the clone are tandemly repeated with a redundancy of 2700 copies per diploid cell.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000378:metabolism*; D005260:Female; D005796:Genes; D007668:Kidney; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000378:metabolism; D010957:Plasmids; D014176:Protein Biosynthesis; D012320:RNA Polymerase III / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics*; D014158:Transcription, Genetic; D014981:Xenopus
D012335:RNA, Ribosomal; D004247:DNA; D012320:RNA Polymerase III