The excitatory effects of the specific benzodiazepine antagonist Ro14-7437, measured intracellularly in hippocampal CA1 cells.
The specific benzodiazepine antagonist, Ro14-7437, in nanomolar concentrations, caused depolarization, increased spontaneous spiking, and conductance decrease when applied to CA1 cells in vitro. These effects were resistant to intracellularly injected Cl- ions or synaptic blockade by TTX, were prevented in Ca2+-free medium, and occurred with or without prior application of midazolam, an inhibitory benzodiazepine. Ca2+-mediated AHPs and Ca2+ spikes in TTX medium were diminished by the blocker, suggesting that Ro14-7437 acted by inhibiting Ca2+-mediated K+ conductance.
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001569:Benzodiazepines / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D004553:Electric Conductivity; D005071:Evoked Potentials / Q000187:drug effects; D006168:Guinea Pigs; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D011188:Potassium / Q000494:pharmacology; D011712:Pyramidal Tracts / Q000187:drug effects / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology
D001569:Benzodiazepines; D013779:Tetrodotoxin; C038674:Ro 14-7437; D011188:Potassium; D002118:Calcium