Metabolism of cartilage proteins in cultured tissue sections.
The biosynthesis and turnover of cartilage proteins was studied in organ cultures of bovine tracheal-cartilage sections. In cultures labelled with [3H]leucine, more than 99% of the labelled macromolecules were retained in the sections. About half of the [3H]leucine-labelled protein was extracted with 4M-guanidinium chloride. The incorporation of [3H]leucine into protein extractable with guanidinium chloride was linear with time, after an initial delay of 20-25 min. The 148kDa and 36kDa cartilage proteins were major labelled components in this extract. The elimination of the proteins was studied by using a pulse-chase protocol. The 148kDa protein was found to have a very slow turnover, similar to that of proteoglycans, whereas the 36kDa protein was eliminated more rapidly.
D000818:Animals; D002356:Cartilage / Q000378:metabolism*; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D046911:Macromolecular Substances; D009924:Organ Culture Techniques; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism
D046911:Macromolecular Substances; D011506:Proteins; D013431:Sulfates; D007930:Leucine