A novel rapid and continuous method for the resolution of cell dispersal activities in crude trypsin preparations.
High speed continuous electrophoretic fractionation of crude commercial trypsin preparations has resulted in the resolution of the cell monolayer dispersal activity into three fractions, two of which were considered useful in the production of fully dispersed single cell suspensions. This separation could be achieved at an input rate for crude enzyme of 500 mg per minute.
D002460:Cell Line; D002469:Cell Separation; D002918:Chymotrypsin / Q000378:metabolism; D004586:Electrophoresis; D006801:Humans; D010196:Pancreatic Elastase / Q000378:metabolism; D014357:Trypsin / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology
D002918:Chymotrypsin; D010196:Pancreatic Elastase; D014357:Trypsin