Use of gelatin/plasma coated flasks for isolating human peripheral blood monocytes.
A simple and efficient technique to purify human peripheral blood monocytes is described. This technique is based on the fact that monocytes have high affinity for fibronectin immobilized on a gelatin coated surface. Cell preparations obtained by the method described are more than 90% monocytes. The phenotype of these adherent cells was characterized with monoclonal antibodies.
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D006224:Cricetinae; D005780:Gelatin; D006801:Humans; D007668:Kidney; D009000:Monocytes / Q000166:cytology* / Q000276:immunology; D010641:Phenotype; D010949:Plasma
D005780:Gelatin