[Commitment to erythroid differentiation and globin gene expression in the absence of heme accumulation in Friend leukemia cells].
Friend leukemia cells (FLC) induced to erythroid differentiation by 5 mM hexamethylenebisacetamide (HMBA) are suitable for studies focused on the expression of different genes involved in the same differentiative program. In our studies FL cells have been cultured in alpha-medium (Gibco), 10% fetal calf serum (FCS, Flow Laboratories). Erythroid differentiation has been analysed by benzidine staining of the cells and globin synthesis by pulse-labelling the cells with 3H-leucine (New England Nuclear) and electrophoresis of newly-synthesized proteins on polyacrylamide gels. Hemoglobin production was determined by cellulose acetate gel electrophoresis. Our results demonstrate that heme synthesis is suppressed by the iron-chelator desferrioxamine. In addition "commitment" to erythroid differentiation and transcription and translation of globin mRNAs occur in HMBA-treated FLC in the absence of heme accumulation.
D000081:Acetamides / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D004588:Electrophoresis, Cellulose Acetate; D005434:Flow Cytometry; D005622:Friend murine leukemia virus; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics*; D006418:Heme / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000235:genetics / Q000503:physiopathology*; D051379:Mice
D000081:Acetamides; D006418:Heme; D005914:Globins; C014026:hexamethylene bisacetamide
"Commitment" al differenziamento eritroide ed espressione dei geni per le globine in assenza di accumulo di eme in cellule leucemiche di Friend.