[Dynamics of the structural organization of the liver parenchymal cells in induced autophagocytosis].
The volume of hepatocytes, their nuclei, cytoplasm, relative and absolute volumes, surface area, number and average volume of cytoplasmic structures have been studied in male mice C57B1 (2-month-old with body mass 19-21 g), 20, 60 min and 72 h after glucagon with glycine buffer in the dose 10 mcg per 100 g of body mass has been injected intraperitoneally. A reverse dependence between glycogen contents and a total volume of cytosegresomes, residual bodies and autophagolysosomes in the hepatic parenchyma is revealed. Acid phosphatase free activity, when hepatic homogenates are treated in 0.25 M and 0.15 M saccharose solutions, is the highest 60 min after glucagon administration. At that time hepatocytes do not contain any glycogen, and volume and number of lysosomes, especially the secondary ones are the greatest. Meanwhile, the total volume of the autophage structures is 150 times as great as the control one. In 60 min the volume of hepatocytes increases nearly by 50%, total volume and number of ultrastructures, especially that of free ribosomes, increase sharply. In 20 min concentration of 11-oxycorticosteroid hormones in blood more than 3 time exceeds the control level. The effect of glucagon dependent on cyclic adenosinmonophosphate is probably realized by means of an enhanced glycogenolysis and an increased level of metabolic processes in the hepatocytes. This causes a deficit of energy substrates, which could, to some extent, be compensated at the expense of reutilization of the lysosomal hydrolysis products.
D000818:Animals; D005934:Glucagon / Q000494:pharmacology; D008099:Liver / Q000502:physiology / Q000648:ultrastructure*; D008112:Liver Glycogen / Q000378:metabolism; D008247:Lysosomes / Q000502:physiology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D010587:Phagocytosis / Q000187:drug effects; D013997:Time Factors
D008112:Liver Glycogen; D005934:Glucagon
Dinamika strukturnoĭ organizatsii kletok parenkhimy pecheni mysheĭ v usloviiakh indutsirovannogo autofagotsitoza.