Serological classification of Haemophilus paragallinarum with a hemagglutinin system.
Antigens, prepared from 17 strains of Haemophilus paragallinarum by treatment with potassium thiocyanate followed by sonication, uniformly agglutinated glutaraldehyde-fixed chicken erythrocytes and formed specific hemagglutination inhibition antibodies in rabbits. Attempts were made to classify the strains into serotypes by a combination of cross-hemagglutination inhibition and cross-absorption tests, using the hemagglutinating antigens, designated as HA-L hemagglutinin, and their antisera. The cross-hemagglutination inhibition tests showed the existence of three distinct groups among the 17 strains. Further cross-absorption studies indicated that two of the three groups could be subdivided into three serotypes each, forming a total of seven serotypes, designated HA-1 through HA-7. Classification based on the serotype-specific HA-L system was found to be superior in its wider and more clearly defined specificities to other previous classifications, which are based on the agglutination test. There appeared to be a correlation between serotypes and geographic origins of the strains.
D000942:Antigens, Bacterial / Q000032:analysis*; D006190:Haemophilus / Q000145:classification* / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000032:analysis*; D013045:Species Specificity
D000942:Antigens, Bacterial; D006388:Hemagglutinins