title: Increased histone mRNA levels during inhibition of protein synthesis.
abstract: Inhibition of protein synthesis by cycloheximide or puromycin specifically increases the amount of translatable histone mRNA in exponentially growing and in synchronous G1 HeLa cells by 5-fold in 3 hours. In this case histone gene expression is uncoupled from DNA replication. We conclude that the level of histone mRNA is regulated by a labile protein and is only indirectly dependent on DNA synthesis.
mesh_terms: D003513:Cycloheximide / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism; D006657:Histones / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007399:Interphase / Q000187:drug effects; D014176:Protein Biosynthesis / Q000187:drug effects*; D011691:Puromycin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D006657:Histones; D012333:RNA, Messenger; D011691:Puromycin; D003513:Cycloheximide