title: Agglutination of mouse erythrocytes by binding of non-choline phospholipids to a 70 000-Dalton protein.
abstract: The structure of some phospholipids that cause agglutination of mouse erythrocytes has been studied. Haemagglutination is a property of non-choline-containing phospholipids; the phosphate group is essential and unsaturated fatty acids optimal. A protein of Mr 70 000 was isolated from mouse erythrocyte membranes which completely inhibited phospholipid-mediated erythrocyte agglutination. It is proposed that this protein is the phospholipid binding site on mouse erythrocytes and the ligand for the human B-lymphocyte receptor for mouse erythrocytes. Preliminary investigations suggest that a similar inhibitor of phospholipid-mediated agglutination is found in serum. Agglutination of mouse erythrocytes by phospholipid and specific inhibition by the 70 kDa membrane protein constitute a simple system for studying the interaction of phospholipid with protein.
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000097:blood*; D002417:Cattle; D004910:Erythrocyte Membrane / Q000276:immunology; D006384:Hemagglutination; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006801:Humans; D008563:Membrane Lipids / Q000097:blood*; D008565:Membrane Proteins / Q000097:blood*; D051379:Mice; D008970:Molecular Weight; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids / Q000097:blood*; D012756:Sheep; D013045:Species Specificity; D013329:Structure-Activity Relationship
chemical_list: D002352:Carrier Proteins; D008563:Membrane Lipids; D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids