title: Brain tubulin and actin cDNA sequences: isolation of recombinant plasmids.
abstract: Rat brain mRNA enriched for tubulin and actin sequences was used to prepare double stranded cDNA. A library of recombinant clones was constructed by inserting the dsDNA into the Pst1 site of pBR322 plasmid and transformation of E. coli chi 1776 host. Clones bearing sequences coding for tubulin and actin were identified and characterized.
mesh_terms: D000199:Actins / Q000096:biosynthesis*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000302:isolation & purification* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D012156:Reticulocytes / Q000378:metabolism; D014404:Tubulin / Q000096:biosynthesis*
chemical_list: D000199:Actins; D004274:DNA, Recombinant; D012333:RNA, Messenger; D014404:Tubulin