Age-related ultrastructural changes in human embryonic lung fibroblasts.
Scanning and transmission electron microscopy were used to examine human embryonic lung fibroblasts at different population doubling levels. Scanning electron microscopy of cells at population doubling levels 26, 45 and 59 did not reveal a significant change in cell size with increasing age. However, transmission electron microscopy of cells at population doubling levels 19 and 45 showed an increase in nuclear lobes, a decrease in the number of ribosomes associated with rough endoplasmic reticulum, and changes to the internal structure of mitochondria on increasing population doubling level. No other previously reported age-related changes were found.
D002460:Cell Line; D002470:Cell Survival; D046508:Culture Techniques; D004622:Embryo, Mammalian; D005260:Female; D005347:Fibroblasts / Q000648:ultrastructure; D006801:Humans; D008168:Lung / Q000166:cytology*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009940:Organoids / Q000648:ultrastructure; D011247:Pregnancy