Effect of partially purified human somatomedin on human fetal and postnatal cartilage in vitro.
Partly purified somatomedin prepared from adult human plasma stimulated [3H]thymidine and [35S]sulphate uptake into human cartilage. At a final concentration of 0.1 U/ml somatomedin the uptake of [3H]thymidine into fetal cartilage at 18 weeks of gestation was 162 +/- 23% compared with controls. Somatomedin also increased the incorporation of [35S]sulphate into fetal cartilage to 137 +/- 22% at 18 weeks compared to controls. The same concentration of somatomedin (0.1 U/ml) also increased the incorporation of [3H]thymidine into postnatal articular cartilage to 239 +/- 69% and [35S]sulphate to 133 +/- 28% compared to controls. Human somatomedin prepared from adult plasma thus has a mitogenic effect on both fetal and postnatal cartilage. The smaller effect on fetal cartilage may reflect different forms of somatomedin and/or differences in receptors for somatomedins on adult and fetal chondrocytes.
D000293:Adolescent; D000328:Adult; D002356:Cartilage / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism; D002358:Cartilage, Articular / Q000187:drug effects / Q000378:metabolism; D002648:Child; D005260:Female; D005333:Fetus / Q000187:drug effects* / Q000378:metabolism; D006128:Growth; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D013002:Somatomedins / Q000494:pharmacology*; D013431:Sulfates / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D013002:Somatomedins; D013431:Sulfates; D014316:Tritium; D013936:Thymidine