Limited benefit to genetically dystrophic chickens from a synthetic proteinase inhibitor: Ep475.
Chickens with inherited muscular dystrophy (Line 413) were treated in two separate trials with daily intraperitoneal injections of 10% DMSO-water solutions containing the proteinase inhibitors, Ep475 and E64. Drug therapy in each case significantly prolonged the functional ability of the treated chickens. Diluent control chickens around day 35 ex ovo characteristically reached a maximum ability to right from the supine position in a standardized functional test for muscle weakness. Subsequently, the control chickens were found to decline progressively in their ability to right. Treatment with the proteinase inhibitors had no effect on the typically elevated levels of plasma creatine kinase activity. In a histological analysis of the affected pectoralis major muscle, drug treatment had no effect on the relative distribution of degenerating, and vacuolated fibers, inflammatory cells, and abnormal fiber diameters. An exception was seen in decreased necrotic fibers of chickens treated with high doses of Ep475. Moreover, both inhibitors had positive effects on two biochemical abnormalities common to the dystrophic pectoralis muscle: increase in noncollagen protein, and reduction in total calcium.
D000818:Animals; D002118:Calcium / Q000032:analysis; D002645:Chickens / Q000235:genetics*; D003402:Creatine Kinase / Q000097:blood; D007930:Leucine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D009043:Motor Activity / Q000187:drug effects; D009124:Muscle Proteins / Q000032:analysis; D009132:Muscles / Q000032:analysis; D009137:Muscular Dystrophy, Animal / Q000097:blood / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D010369:Pectoralis Muscles / Q000473:pathology; D011480:Protease Inhibitors / Q000627:therapeutic use*
D009124:Muscle Proteins; D011480:Protease Inhibitors; C032425:N-(N-(3-carboxyoxirane-2-carbonyl)leucyl)isoamylamine; D003402:Creatine Kinase; D007930:Leucine; D002118:Calcium