Uptake of adenosine into cultured cerebral endothelium.
Adenosine uptake by cultured cerebral endothelium is a carrier-mediated process. The Km value for adenosine uptake is 5.0 microM and the vmax is 1.15 nmol/min/mg cell protein. The uptake system is inhibited by the adenosine analog 2-chloroadenosine at low adenosine concentrations. The results prove the existence of a nucleoside transport system associated with cerebral capillary endothelium.
D015762:2-Chloroadenosine; D000241:Adenosine / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002250:Carbon Radioisotopes; D002460:Cell Line; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation / Q000187:drug effects; D004176:Dipyridamole / Q000494:pharmacology; D004727:Endothelium / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D010208:Papaverine / Q000494:pharmacology; D013395:Sucrose / Q000378:metabolism; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; D015762:2-Chloroadenosine; D013395:Sucrose; D004176:Dipyridamole; D010208:Papaverine; D000241:Adenosine