Induction of squamous metaplasia in organ cultures of hamster trachea by naturally occurring and synthetic fibers.
Asbestos exhibits many properties of classical tumor promoters. These characteristics include the ability to stimulate proliferation and inhibit normal differentiation of cells. In organ cultures of trachea, crocidolite and amosite asbestos stimulate squamous metaplasia, a pathological process in which a rapidly proliferating squamous epithelium replaces the normal epithelium. We hypothesized that the induction of metaplasia depends upon the fibrous nature of asbestos. Accordingly, several naturally occurring and synthetic fibrous materials and their nonfibrous analogues were assessed for their ability to induce metaplastic changes in tracheal mucosa of the Syrian hamster. Exposure to both crocidolite asbestos and fiberglass resulted in significant increases (p less than 0.05) in squamous metaplasia over a range of dosages (1.0, 4.0, 16.0 mg/ml). Attapulgite (palygorskite) and both "long-" and "short-" fiber preparations of chrysotile asbestos had similar but less marked effects. Nonfibrous analogues of each material (riebeckite, antigorite, and glass particles) failed to produce metaplasia. Asbestos, and fibrous materials in general, appear to stimulate squamous metaplasia because of their fibrous geometry.
D000818:Animals; D001194:Asbestos; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced* / Q000473:pathology; D006224:Cricetinae; D005898:Glass; D008274:Magnesium; D017616:Magnesium Compounds; D008855:Microscopy, Electron, Scanning; D009924:Organ Culture Techniques; D012825:Silicon; D017655:Silicon Compounds; D013329:Structure-Activity Relationship; D014134:Tracheal Neoplasms / Q000139:chemically induced* / Q000473:pathology
D017616:Magnesium Compounds; D017655:Silicon Compounds; C009753:fiberglass; D001194:Asbestos; D008274:Magnesium; C026325:attapulgite; D012825:Silicon