title: A possible complementary role of actin microfilaments and microtubules in triacylglycerol secretion by isolated rat hepatocytes.
abstract: Incubation of isolated rat hepatocytes with phalloidin, cytochalasins (which, respectively, stabilize and destabilize actin microfilaments), or colchicine (which inhibits polymerization of microtubules), resulted in a dose-dependent inhibition of triacylglycerol secretion (an index of very low density lipoprotein secretion). Upon removal of drugs from incubation media, the inhibitory effect of cytochalasin D on triacylglycerol secretion was reversible, while such was not the case of phalloidin. When used at maximal concentrations, the combined presence of phalloidin + colchicine or cytochalasin D + colchicine had additive inhibitory effects upon hepatic triacylglycerol secretion, which was virtually blocked; this was not the case for phalloidin + cytochalasin D. These experiments support the concept that microfilaments and microtubules may have complementary functions for the hepatic secretion of very low density lipoproteins.
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D003078:Colchicine / Q000494:pharmacology; D003572:Cytochalasins / Q000494:pharmacology; D003599:Cytoskeleton / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008870:Microtubules / Q000378:metabolism*; D010590:Phalloidine / Q000494:pharmacology; D051381:Rats; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D000199:Actins; D003572:Cytochalasins; D014280:Triglycerides; D010590:Phalloidine; D003078:Colchicine