Assay of detergents by rocket electrophoresis in agarose gels containing red blood cells: "rocket hemolysis".
A method is described for quantitation of charged detergents using their hemolytic property in an electrophoresis assay in agarose gels containing red blood cells. After electrophoresis the zone of hemolysis is directly proportional to the concentration of detergent in the sample. Using this technique we have determined the smallest detectable concentration for the negatively charged detergents, sodium dodecyl sulfate (SDS) and Quil A to about 10 micrograms/ml and 25 micrograms/ml, respectively and the positively charged cetyltrimethylammonium bromide (CTAB) to about 10 micrograms/ml.
D000818:Animals; D001681:Biological Assay; D003902:Detergents / Q000032:analysis* / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004912:Erythrocytes / Q000166:cytology*; D006461:Hemolysis / Q000187:drug effects*; D007700:Kinetics; D012756:Sheep; D013501:Surface-Active Agents / Q000032:analysis*
D003902:Detergents; D013501:Surface-Active Agents