Extracts of cortical bone from adult rats stimulate DNA synthesis in osteoprogenitor cells from fetal rats.
DNA-synthesis-stimulating activity is stored in cortical bone of normal adult rats. Devitalized cortical bone of adult rats extracted with 0.6 N HCl-4M guanidine HCl solution stimulates DNA synthesis in calvarial osteoprogenitor cells from fetal rats. This activity is heat stable and resides in reduction-labile protein with molecular weights of 13,700-25,000 daltons (Sephacryl S-200 gel column chromatography without reduction). The active fraction purified by gel filtration contains several proteins with molecular weights of 14,400-30,000 daltons (SDS-polyacrylamide gel electrophoresis with reduction).
D000818:Animals; D002478:Cells, Cultured; D002850:Chromatography, Gel; D004247:DNA / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005333:Fetus; D006133:Growth Substances / Q000032:analysis*; D007335:Insulin-Like Growth Factor II; D010006:Osteoblasts / Q000378:metabolism*; D011506:Proteins / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D006133:Growth Substances; C035170:IGF2 protein, human; D011506:Proteins; D007335:Insulin-Like Growth Factor II; D004247:DNA