[Luminescence absorption properties of the olivomycin molecule and its complex with DNA].
Olivomycin (I) is a fluorescent probe which is highly specific with respect to DNA. Its luminescence-absorption properties were studied at various pH values, in different solvents and in the presence of Mg2+ and DNA in the solutions. The presence of 2 tautomeric forms of I was suggested on the basis of the study (forms A and B having different characteristics with respect to the absorption spectra and fluorescence). Transition between forms A and B occurred in both the basic and the excited states. The quantitative ratio of the forms depended on the character of the environment. The presence of the 2 forms of I should be considered in analysis of the drug pharmacokinetics and pharmacodynamics.
D002413:Cations, Divalent / Q000494:pharmacology; D004247:DNA / Q000032:analysis*; D004338:Drug Combinations; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements; D008274:Magnesium / Q000494:pharmacology; D009848:Olivomycins / Q000032:analysis*; D012997:Solvents / Q000494:pharmacology; D013050:Spectrometry, Fluorescence
D002413:Cations, Divalent; D004338:Drug Combinations; D012997:Solvents; D009848:Olivomycins; D004247:DNA; D008274:Magnesium
Liuminestsentno-absorbtsionnye svoĭstva molekuly olivomitsina i ee kompleksa s DNK.