Identification of antigenic components of Toxoplasma gondii by an immunoblotting technique.
Proteins of Toxoplasma gondii were separated by SDS-polyacrylamide gel electrophoresis with subsequent transfer to a nitrocellulose sheet by electrophoretic blotting. Immunologically reactive polypeptides were detected by human sera with previously known toxoplasma antibody levels. Heavy chain-specific, peroxidase-conjugated anti-human immunoglobulins were used as the indicator antibodies for the separate identification of IgG and IgM reactive polypeptides. IgG toxoplasma antibodies reacted with several antigens of Mr approximately 27 000-67 000, while toxoplasma-specific IgM seemed to detect only a few polypeptides. The Mr of 35 000 for the dominating IgM reactive polypeptide was observed.
D000906:Antibodies / Q000302:isolation & purification; D000941:Antigens / Q000302:isolation & purification*; D006801:Humans; D007120:Immunochemistry; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D010455:Peptides / Q000276:immunology; D014122:Toxoplasma / Q000276:immunology*
D000906:Antibodies; D000941:Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010455:Peptides