title: Characterization of aromatic heptaene macrolide antibiotics by high performance liquid chromatography.
abstract: High performance liquid chromatographic (HPLC) procedures were utilized for the rapid and efficient separation and characterization of the aromatic heptaene macrolide group of antifungal antibiotics. The instrument utilized a 350 nm phosphor converted ultraviolet detector and a mu Bondapak C18 column packing. Optimum resolution of eleven commercial aromatic heptaene macrolide preparations was obtained with solvent systems consisting of mixtures of acetonitrile and 0.05 M aqueous sodium citrate buffer, pH 5.3. The presence of two distinct types of aromatic heptaene macrolides with numerous well-defined individual components has been established.
mesh_terms: D000935:Antifungal Agents / Q000032:analysis*; D002174:Candicidin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009930:Organic Chemicals; D011090:Polyenes / Q000032:analysis*
chemical_list: D000935:Antifungal Agents; D009930:Organic Chemicals; D011090:Polyenes; C100293:partricin; D002174:Candicidin; C100192:hachimycin