title: Effects of phrenic nerve section on the respiratory system of fetal lambs.
abstract: We investigated the effects of phrenic nerve section (PNS) on the respiratory system of fetal lambs. Seven ewes, three of which had twin fetuses, were given a general anesthetic. The thoracic phrenic nerves were cut in two singleton fetuses and in one fetus in each set of twins (116-121 days); two singleton fetuses and one fetus in each set of twins underwent the same procedure except for PNS. Fetal arterial blood pressure, heart rate, and arterial pH and blood gas tensions were the same in both groups. Phrenic nerve section eliminated fetal breathing movements and decreased airway fluid volume, lung weight, and total lung DNA (P less than 0.05). However, PNS did not affect production of tracheal fluid or percent dry weight of the lungs. Furthermore, PNS did not affect the concentration of saturated phosphatidylcholine in the lung or its flux in tracheal fluid. We conclude that PNS in fetal lambs retards lung growth but does not affect tracheal fluid production or formation and release of surfactant.
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis; D008168:Lung / Q000032:analysis / Q000196:embryology*; D009929:Organ Size; D010791:Phrenic Nerve / Q000502:physiology*; D011663:Pulmonary Surfactants / Q000378:metabolism; D012119:Respiration; D012756:Sheep; D014132:Trachea / Q000196:embryology
chemical_list: D011663:Pulmonary Surfactants; D004247:DNA