Contribution to knowledge of the biosynthesis of cyclosporin A.
3H- and 13C-NMR spectroscopic investigations on the structure of labeled cyclosporin A were performed after feeding of appropriate precursors. The 6 N-methyl groups and the methyl group in position 4 of the epsilon, zeta-unsaturated amino acid No. 1 (Mebmt) are introduced as intact CH3-units from methionine. Four head-to-tail acetate units are involved in the biosynthesis of the 8-carbon chain of the olefinic amino acid.
D000085:Acetates / Q000378:metabolism; D002247:Carbon Isotopes; D003524:Cyclosporins / Q000096:biosynthesis*; D003903:Deuterium; D005658:Fungi / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008715:Methionine / Q000378:metabolism; D014316:Tritium
D000085:Acetates; D002247:Carbon Isotopes; D003524:Cyclosporins; D014316:Tritium; D008715:Methionine; D003903:Deuterium