Intracellular pH and free calcium changes in single cells using quene 1 and quin 2 probes and fluorescence microscopy.
Photometric fluorescence microscopy has been used to measure intracellular pH (pHi) and free calcium concentrations [( Ca]i) in individual mouse thymocytes and 2H3 rat basophil leukaemic cells containing indicators for pH (quene 1) or calcium (quin 2). The pHi and [Ca]i measurements in individual 2H3 cells and mouse thymocytes and their responses to various stimuli were consistent with the corresponding data obtained from suspensions of these cells measured in a spectrofluorimeter. Photometric fluorescence microscopy of these indicators in individual cells provides a sensitive and fast method of following pHi and [Ca]i responses in individual cells.
D000634:Aminoquinolines; D000818:Animals; D001491:Basophils / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007942:Leukemia, Experimental / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence; D013950:Thymus Gland / Q000378:metabolism
D000634:Aminoquinolines; D005456:Fluorescent Dyes; C038108:8-(bis(ethoxycarbonylmethyl)amino)-6-methoxy-2-(bis(ethoxycarbonylmethyl)amino)styrylquinoline; D002118:Calcium