Homokaryon production by electrofusion: a convenient way to produce a large number of viable mammalian fused cells.
A convenient technique to obtain homokaryons is described that provides large amounts of fused mammalian cells. Chinese hamster ovary cells grown in monolayers on a Petri dish are submitted to square wave electric pulses. Viability of cells is observed not to be affected by this electric treatment. The yield of fusion is strongly dependent on the strength of the field (KV/cm range) and on the duration of the pulse (microsecond range). The yield is not improved by accumulation of pulses. Yields up to 80% are obtained and under our experimental conditions 200 000 cells are fused per assay.
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002467:Cell Nucleus / Q000502:physiology; D002470:Cell Survival; D006224:Cricetinae; D003412:Cricetulus; D004558:Electric Stimulation; D005260:Female; D007700:Kinetics; D008722:Methods; D010053:Ovary