title: New methods of purifying and identifying parasitic diagnostic antigens: a review of the systematic and quantitative approach with the Schistosoma model.
abstract: Several antigen preparations intended for use in the serodiagnosis of schistosomiasis were reviewed. The efficacies of these antigens in terms of specific activities and cross-reactivities were compared. The idea of quantitation of the actual usable amount of antigens in various preparations was introduced. A systematic approach to the purification and identification of relevant antigens in the Schistosoma system was discussed. Use of the quantitative single-tube kinetic-dependent enzyme-linked immunosorbent assay in conjunction with a systematic approach to antigen purification allowed for the identification of substantial activities in membrane-associated antigens. Solubilization of the particulate- and presumably membrane-associated components resulted in significant amounts of highly sero-reactive antigens. Total reliance on aqueous-soluble parasitic antigens is questioned.
mesh_terms: D000818:Animals; D000941:Antigens / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D010063:Ovum / Q000276:immunology; D012547:Schistosoma / Q000276:immunology*; D012549:Schistosoma japonicum / Q000276:immunology; D012550:Schistosoma mansoni / Q000276:immunology; D012552:Schistosomiasis / Q000175:diagnosis*; D013347:Subcellular Fractions / Q000276:immunology
chemical_list: D000941:Antigens