The state of neutral lipids in normal and cataractous human lenses.
A simple and rapid high pressure liquid chromatographic method (HPLC) was developed to analyse the neutral lipid fraction from normal and cataractous human lenses. No differences were found between old normal and cataractous lenses suggesting that membrane deterioration in cataracts is not due to oxidation of these components. Several new lens components were isolated and identified including cholesterol metabolites, and vitamin E (alpha-tocopherol).
D000328:Adult; D000368:Aged; D000375:Aging; D002386:Cataract / Q000378:metabolism*; D002518:Ceramides / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D066298:In Vitro Techniques; D007810:Lanosterol / Q000031:analogs & derivatives / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism*; D050356:Lipid Metabolism; D008875:Middle Aged; D014810:Vitamin E / Q000378:metabolism
D002518:Ceramides; D014810:Vitamin E; D007810:Lanosterol; C015316:lanostenol; D002784:Cholesterol