Metabolic behavior of acetyl glyceryl ether phosphorylcholine on interaction with rabbit platelets.
The metabolic fate of 1-O-[3H]alkyl-2-acetyl-sn-glycero-3-phosphorylcholine ([3H]-AGEPC) upon interaction with rabbit platelets was investigated. [3H]AGEPC was converted to a product identified as the long-chain fatty acyl analog. The reaction was unaffected by extracellular calcium. After a lag time of 30 to 60 s the kinetics of the conversion was linear. The rate of the reaction was found to be a function of platelet and AGEPC concentrations. Of the [3H]AGEPC (10(-9) M) 85 +/- 5% was processed into the-long chain fatty acyl analog within 1 h when incubated at 37 degrees C with a 1.25 X 10(9) platelets per milliliter suspension. A maximal number of 1200 to 3600 [3H]AGEPC molecules were converted to the long-chain fatty acyl derivative per minute per platelet in the presence of 2 mM EDTA. Under similar conditions the 1-O-[3H]alkyl-2-(lyso)-sn-glycero-3-phosphorylcholine ([3H]lysoGEPC) also was transformed to a comparable long-chain fatty acyl derivative at a much slower rate and to a lower extent. No significant increase in lysoGEPC was noted in incubation mixtures containing [3H]AGEPC. The possible direct transacylation of AGEPC upon interaction with platelets is discussed as well as the possible involvement of this reaction in directly triggering the platelet response to AGEPC stimuli.
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D066298:In Vitro Techniques; D008658:Inactivation, Metabolic; D010713:Phosphatidylcholines / Q000096:biosynthesis / Q000097:blood; D010743:Phospholipids / Q000097:blood; D010972:Platelet Activating Factor / Q000378:metabolism*; D011817:Rabbits
D010713:Phosphatidylcholines; D010743:Phospholipids; D010972:Platelet Activating Factor