title: Derivatization of polyacrylamide gels with pallidin, a lectin of the cellular slime mold polysphondylium pallidum.
abstract: A procedure for covalent immobilization of (glyco) proteins in polyacrylamide gels is described. Pallidin, a cytosolic and membrane protein of Polysphondylium pallidum with the properties of a lectin, was derivatized into polyacrylamide gels. Activity of the derivatized gels was assessed in a cell adhesion assay, using rabbit erythrocytes. Immobilization resulted in stabilization of the binding activity in comparison with the agglutination activity of the soluble protein.
mesh_terms: D000180:Acrylic Resins; D000818:Animals; D002352:Carrier Proteins; D055598:Chemical Phenomena; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004912:Erythrocytes / Q000378:metabolism; D037102:Lectins; D011817:Rabbits; D000267:Tissue Adhesions
chemical_list: D000180:Acrylic Resins; C400284:BLOC1S6 protein, human; D002352:Carrier Proteins; D037102:Lectins; C016680:polyacrylamide gels