Synthesis and application of two reagents for the introduction of sulfhydryl groups into proteins.
Two reagents are described which can be used for the introduction of sulfhydryl groups into proteins. Mercaptopropionylhydrazide modifies specifically periodate-oxidized N termini of proteins, provided that the N-terminal residue is serine or threonine. 3-(Phenyldithio)propionimidate introduces a disulfide bond at lysine residues of proteins. Reduction converts the disulfide into a sulfhydryl group. The imidate compound was found to react with a high specificity with only one lysine residue of ribosomal protein L7/L12.
D000603:Amino Acids, Sulfur / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000494:pharmacology*; D007202:Indicators and Reagents; D007525:Isoelectric Focusing; D012269:Ribosomal Proteins / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D008625:Tiopronin / Q000031:analogs & derivatives / Q000494:pharmacology*
C038871:3-(phenyldithio)propionimidate; D000603:Amino Acids, Sulfur; D004220:Disulfides; D007202:Indicators and Reagents; D012269:Ribosomal Proteins; D013438:Sulfhydryl Compounds; C038870:mercaptopropionylhydrazide; D008625:Tiopronin