Intracellular and extracellular ion content of the endolymphatic sac.
The Cl- activity in the endolymph of the endolymphatic sac and in the cochlear duct was measured with Cl- sensitive double-barreled microelectrodes. The Cl- activity in the endolymphatic sac fluid was lower than in the cochlear duct. A small, positive, DC potential was recorded in the endolymphatic sac. During anoxia, the DC potential decreased while the Cl- activity in the endolymphatic sac increased. The K/Na ratio in the epithelial cells and subepithelial tissue of endolymphatic sac was measured using the LAMMA technique. The K/Na ratio in the epithelial cells decreased after ethacrynic acid injection (60 mg/kg i.v.). These findings suggest that chloride in the endolymphatic sac is actively transported inward and outward.
D000818:Animals; D002712:Chlorides / Q000032:analysis*; D003053:Cochlear Duct / Q000032:analysis; D007758:Ear, Inner / Q000032:analysis; D004710:Endolymph / Q000032:analysis*; D004712:Endolymphatic Sac / Q000032:analysis; D006168:Guinea Pigs; D007761:Labyrinthine Fluids / Q000032:analysis*; D008839:Microelectrodes
D002712:Chlorides