Hyaluronic acid and proteoglycan synthesis by lung fibroblasts in basal and activated states.
Most glycosaminoglycans (GAGs) formed by lung fibroblasts under both basal and stimulated conditions were secreted into the culture medium. High molecular weight (greater than 10(6) daltons) hyaluronic acid (HA) was the major GAG species formed. Most of the SO4-GAG synthesized by lung fibroblasts was of proteoglycan (PG) monomer size. Agents stimulating complex carbohydrate formation also exhibited some selectivity with respect to whether HA or PG was formed in incremental amounts. Hyaluronate synthesis was especially stimulated by endotoxins, SO4-GAG by beta-xylosides, and PG by CTAP-III.
D000818:Animals; D002478:Cells, Cultured; D004731:Endotoxins / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006027:Glycosides / Q000494:pharmacology; D006168:Guinea Pigs; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology; D011509:Proteoglycans / Q000096:biosynthesis*
D004731:Endotoxins; D006025:Glycosaminoglycans; D006027:Glycosides; D010455:Peptides; D011509:Proteoglycans; C010807:xylosides; C009862:connective tissue-activating peptide; D006820:Hyaluronic Acid