title: Estimation of volatile N-nitrosamines in rubber nipples for babies' bottles.
abstract: A method is described for the estimation of volatile N-nitrosamines in the rubber nipples of babies' bottles. In a study of rubber nipples from one manufacturer, N-nitrosodimethylamine, N-nitrosodiethylamine and N-nitrosopiperidine were determined by gas chromatography, using a thermal energy analyser, and their presence was confirmed by mass spectrometry with average levels of individual nitrosamines ranging from 22 to 281 ppb. When the nipples were sterilized in a conventional sterilizer together with milk or infant formula the three nitrosamines migrated into the milk or formula. Storing a bottle of milk with a rubber nipple inverted in it for 2 hr at room temperature or overnight in a refrigerator after sterilization resulted in an 8-13% average increase in the nitrosamine levels migrating into the milk. On repeated sterilization of a single nipple, the quantities of nitrosamines migrating into milk from rubber nipples declined steadily, but after seven sterilizations, nitrosamines were still readily detectable in the milk. Nitrosamine levels were higher in rubber nipples after sterilization, indicating the presence of nitrosamine precursors in the nipples. No nitrosamines were found in raw, uncured rubber. Chemical accelerators and stabilizers added during the vulcanization process are the source of the amine precursors in rubber nipples.
mesh_terms: D001903:Bottle Feeding; D006801:Humans; D007223:Infant; D007225:Infant Food; D007231:Infant, Newborn; D009602:Nitrosamines / Q000302:isolation & purification*; D012408:Rubber / Q000032:analysis*; D014835:Volatilization
chemical_list: D009602:Nitrosamines; D012408:Rubber