Plasma free and sulfate conjugated catecholamine levels during acute physiological stimulation in man.
The responses of plasma free and sulfate-conjugated catecholamines to acute physiological stimulation was examined in normal male subjects. Catecholamines were measured with a sensitive radioenzymatic assay incorporating simultaneous hydrolysis of sulfate conjugates and O-methylation of free norepinephrine and epinephrine. Following 20 minutes recumbency after venepuncture 30 +/- 3% of norepinephrine and 16 +/- 5% of epinephrine was in thr free form. Free catecholamines generally increased during standing, cold immersion and isometric handgrip, but sulfates did not change. Bicycle ergometry markedly increased free catecholamines which rapidly returned to basal levels at the end of exercise. In contrast, sulfated norepinephrine decreased substantially with exercise in all subjects but returned to basal levels 3 minutes after stopping exercise. Epinephrine sulfate varied considerably between subjects but showed a similar, although smaller, fall with exercise. Thus, during physiological stimulation, which caused increases in free norepinephrine and epinephrine levels in plasma, the only consistent change in sulfated catecholamines was a marked fall in norepinephrine sulfate after bicycle exercise. This may indicate saturation of sulfotransferase activity, substrate inhibition or impaired tissue conjugation.
D000328:Adult; D002395:Catecholamines / Q000097:blood*; D004837:Epinephrine / Q000097:blood; D006801:Humans; D008297:Male; D009638:Norepinephrine / Q000097:blood / Q000031:analogs & derivatives; D005082:Physical Exertion; D011187:Posture
D002395:Catecholamines; C017574:noradrenaline sulfate; D009638:Norepinephrine; D004837:Epinephrine