[Immunological and morphological properties of fibroblasts transformed in vitro. I. The isolation of continuous mouse cell lines, their malignancy, H-2 antigens and complement sensitivity].
7 continuous cell lines with different degrees of malignancy were obtained from cultures of embryo fibroblasts of 4 strains of mice. Three of them were selected in vivo for enhanced malignancy. A study of regularities of changes in immunologic and some cytologic characteristics of these cells in the processes of transformation and malignization has been started. The data are presented that in these processes the density of H-2 antigens in embryo fibroblasts many vary significantly in both directions. These alterations are stochastic and do not depend on transformation or malignization of cells. It is also shown that the sensitivity of the cells under study to the complement lytic action may significantly vary, and does not always correlate with the density of antigens which are the targets of the complement lytic action.
D000818:Animals; D002460:Cell Line; D002469:Cell Separation; D002471:Cell Transformation, Neoplastic / Q000276:immunology* / Q000473:pathology; D003165:Complement System Proteins / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D005347:Fibroblasts / Q000276:immunology* / Q000473:pathology; D005838:Genotype; D006183:H-2 Antigens / Q000032:analysis*; D007114:Immunization; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology / Q000473:pathology; D013997:Time Factors
D006183:H-2 Antigens; D003165:Complement System Proteins
Immunologicheskie i morfologicheskie svoĭstva fibroblastov, transformirovannykh in vitro. I. Poluchenie dlitel'no perevivaemykh myshinykh kletochnykh liniĭ, ikh zlokachestvennost', H-2-antigeny, chuvstvitel'nost' k komplementu.