Transmembrane potential of J774.2 mouse macrophage cells measured by microelectrode and ion distribution methods.
The transmembrane potential (Em) of J774.2 macrophage cells measured by microelectrodes was -24.1 +/- 0.7 mV (mean +/- SEM). Em measured by lipophilic ion distribution was -35 +/- 2 mV or -40 +/- 2 mV, using a cation or anion, respectively. By any method, colchicine reduced Em by approximately 3 mV.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D004553:Electric Conductivity; D008264:Macrophages / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008839:Microelectrodes; D011188:Potassium / Q000494:pharmacology
D011188:Potassium