Cell beta-actinin, an accelerator of actin polymerization, isolated from rat kidney cytosol.
A motility-regulatory protein called cell beta-actinin was isolated from rat kidney cytosol. Although its molecular weight (approximately 80,000) is different from that of beta-actinin from skeletal muscle (heterodimer of 34,000 and 37,000), the action on actin is the same for both. Cell beta-actinin accelerates polymerization of actin by enhanced nucleation resulting in shorter actin filaments, inhibits the reassociation of sonicated F-actin fragments, and forms a Mg polymer in the presence of Mg ions. Cell beta-actinin appears to be present in various tissues of rat, rabbit, chicken, and pig. Cell beta-actinin is thought to function as an accelerator of actin polymerization in nonmuscle cells, in which actin monomers are abundantly present.
D000185:Actinin / Q000302:isolation & purification* / Q000378:metabolism; D000199:Actins / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D002850:Chromatography, Gel; D003600:Cytosol / Q000032:analysis; D007668:Kidney / Q000032:analysis*; D008274:Magnesium / Q000378:metabolism; D008854:Microscopy, Electron; D009124:Muscle Proteins / Q000302:isolation & purification*; D011817:Rabbits; D051381:Rats; D013552:Swine; D014018:Tissue Distribution
D000199:Actins; D009124:Muscle Proteins; D000185:Actinin; D008274:Magnesium