title: Investigation on the pattern of polar lipids extracted from different strains of cultured cells.
abstract: The polar lipid patterns of several cell strains (EUE, L929, MT, RAG and TAZA) were compared by TLC separation in order to identify a typical lipid profile as a possible "strain marker". Phospholipids and sulfolipids were tested by colorimetric procedures. EUE and L929 contain smaller amounts of sulfolipids (2.4-3.9; 3.0-4.5) than RAG (4.4-12.0) or MT (10.5) (micrograms x 10(-6)/cell). MT and RAG contain higher phospholipid P concentration than other strains. (MT:14.0 whilst EUE 1.3-1.8 micrograms phospholipidic P x 10(-6)/cell). The patterns of lipidograms in the characterization of two cell strains are discussed in terms of their usefulness as rapid "fingerprint" in biochemical and histochemical analysis.
mesh_terms: D000230:Adenocarcinoma; D000818:Animals; D002460:Cell Line; D002855:Chromatography, Thin Layer / Q000379:methods; D004622:Embryo, Mammalian; D004848:Epithelium; D006801:Humans; D007680:Kidney Neoplasms; D007739:L Cells / Q000032:analysis; D008055:Lipids / Q000302:isolation & purification*; D008297:Male; D008545:Melanoma; D051379:Mice; D010743:Phospholipids / Q000302:isolation & purification; D051381:Rats; D013737:Testis
chemical_list: D008055:Lipids; D010743:Phospholipids