Reversed-phase ion-pair liquid chromatographic procedure with electrochemical detection for the analysis of urinary thiosulphate.
A method using high-performance liquid chromatography with mercury-based electrochemical detection has been developed for the determination of thiosulphate in urine. The chromatographic separation is based upon ion-pair formation between thiosulphate and tetrabutylammonium and reversed-phase chromatography. The method was compared with an earlier reported colorimetric assay and found to be superior with respect to specificity and sensitivity.
D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D004563:Electrochemistry; D006801:Humans; D000644:Quaternary Ammonium Compounds; D013885:Thiosulfates / Q000652:urine*; D014947:Wounds and Injuries / Q000188:drug therapy / Q000652:urine
D000900:Anti-Bacterial Agents; D000644:Quaternary Ammonium Compounds; D013885:Thiosulfates; C009405:tetrabutylammonium