Purification of human tissue-type plasminogen activator in centigram quantities from human melanoma cell culture fluid and its conditioning for use in vivo.
Human tissue-type plasminogen activator was produced in centigram quantities from 50-60 1 batches of conditioned medium of a human melanoma cell culture. The yields of the procedure remained essentially unchanged during 50 subsequent preparations. The final products were of high purity as assessed by SDS gel electrophoresis. These materials, after filtration on 0.22 microM Milliporefilters were sterile and free of viruses and pyrogens. Their stability was very good in the fluid form at temperatures up to 56 degrees C. Material obtained by the present procedure has been used to investigate the biological and thrombolytic properties of tissue-type plasminogen activator.
D002460:Cell Line; D003470:Culture Media; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008545:Melanoma / Q000032:analysis*; D010960:Plasminogen Activators / Q000302:isolation & purification*
D003470:Culture Media; D010960:Plasminogen Activators