Microfilament assembly during lens cell elongation in vitro.
Bovine epitheloid lens cells can be kept in culture for almost one year. During subculturing a gradual process of cell elongation is observed. Using several techniques, including immunofluorescence with antibodies against actin, electron microscopy, two-dimensional gel electrophoresis, drug treatment and measurement of actin polymerization by DNase I inhibition, it is shown that microfilament assembly parallels the process of cell elongation.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003459:Crystallins / Q000096:biosynthesis; D003599:Cytoskeleton / Q000648:ultrastructure*; D004847:Epithelial Cells; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning
D003459:Crystallins