title: Measurement of anionic sites of rat epididymal spermatozoa using tritiated polycationized ferritin.
abstract: Anionic sites of rat epididymal spermatozoa were measured at pH 7.4 using tritiated polycationized ferritin. The spermatozoa from the caput region had 1.25 +/- 0.06 X 10(6) anionic sites per cell and a binding constant of 1.26 +/- 0.01 X 10(6) M-1. Spermatozoa from the cauda region had 1.50 +/- 0.09 X 10(6) anionic sites per cell and a binding constant of 4.84 +/- 0.82 X 10(6) M-1. The values were mean +/- s.d. The anionic sites were partly sensitive to treatments with neuraminidase, trypsin and Triton X-100.
mesh_terms: D000818:Animals; D000838:Anions / Q000378:metabolism*; D001665:Binding Sites / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology; D004563:Electrochemistry; D004822:Epididymis; D005293:Ferritins / Q000378:metabolism*; D008297:Male; D009439:Neuraminidase / Q000494:pharmacology; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism*; D014316:Tritium; D014357:Trypsin / Q000494:pharmacology
chemical_list: D000838:Anions; D003902:Detergents; C010297:polycationic ferritin; D014316:Tritium; D011092:Polyethylene Glycols; D017830:Octoxynol; D005293:Ferritins; D009439:Neuraminidase; D014357:Trypsin