A rapid method for embedding tissues for electron microscopy using 1,4-dioxane and Polybed 812.
A rapid method for embedding tissues for electron microscopy is described. This method, which can be completed within 5 hr, uses 1,4-dioxane as the final dehydrating agent and Polybed 812 as the embedding medium. Satisfactory preservation of cellular structures is consistently achieved with a variety of normal and diseased tissues. This method may be of particular value to diagnostic electron microscopy laboratories where time and simplicity are critical.
D000818:Animals; D004146:Dioxanes; D004147:Dioxins; D008854:Microscopy, Electron / Q000379:methods*; D009000:Monocytes / Q000648:ultrastructure; D009393:Nephritis / Q000473:pathology; D011108:Polymers; D011817:Rabbits; D051381:Rats; D012713:Serum Sickness / Q000473:pathology; D013154:Spleen / Q000648:ultrastructure
D004146:Dioxanes; D004147:Dioxins; D011108:Polymers; C025222:Polybed 812; C025223:1,4-dioxane