[Influenza virus proteins. Incorporation of newly synthesized viral proteins into virions].
Incorporation of individual viral proteins (P1, P2, P3, NP, NA, HA, and M) into virions was studied by the "pulse-chase" method followed by electrophoretic analysis of the proteins in chick fibroblast cultures infected with influenza WSN virus. Viral proteins were found to incorporate into virions rather rapidly and for a long time after synthesis, with the exception of M protein incorporation of which into the virus markedly decreased by 4 hours after synthesis. Nucleocapsid proteins (NP, P1, P2, and P3) incorporated into the virus asynchronously: by 17 hours postinoculation, in NP protein prevailed the molecules synthesized after 6 hours of infection, in P2 protein the molecules synthesized in the first hours of infection, and P1 and P3 proteins were represented by a uniform set of molecules synthesized at various periods after inoculation. The evidence on heterogeneity of P2 and M proteins in the virus has been obtained. Possible mechanisms regulating the selection of molecules of individual viral proteins in formation of virus particles are discussed.
D000818:Animals; D002213:Capsid / Q000378:metabolism; D002478:Cells, Cultured; D002645:Chickens; D009975:Orthomyxoviridae / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D014771:Virion / Q000378:metabolism*
D014764:Viral Proteins
Belki virusa grippa. Vkliuchenie vnov' sintezirovannykh virusnykh belkov v viriony.