Intrathecal morphine in mice: a new technique.
A simple, rapid technique for intrathecal injections by lumbar puncture in unanesthetized mice is described. Intrathecal [3H]morphine base was not found in significant quantities in either the midbrain or forebrain. Submicrogram quantities of morphine sulfate induced Straub tail response and tail-flick analgesia. These effects were dose related and antagonized by subcutaneous naloxone.
D000698:Analgesia; D000818:Animals; D004305:Dose-Response Relationship, Drug; D007278:Injections, Spinal; D008297:Male; D051379:Mice; D009020:Morphine / Q000008:administration & dosage* / Q000494:pharmacology; D013116:Spinal Cord / Q000187:drug effects*
D009020:Morphine