title: Effect of streptozotocin-induced diabetes on the metabolism of ADP, AMP and adenosine in the pulmonary circulation of rat isolated lung.
abstract: The uptake and metabolism of radioactive ADP, AMP and adenosine was investigated in the pulmonary circulation of lungs taken from rats with streptozotocin-induced diabetes. Between 0.26 and 500 mumol/l ADP was extensively hydrolysed to AMP equally in control and diabetic lungs. At 1 mmol/l there was less ADP breakdown in diabetic lungs. Hydrolysis of AMP to adenosine was also less in diabetic lungs at 10 mumol/l and 1 mmol/l substrate concentration, but adenosine metabolism and uptake at these concentrations was not affected by diabetes. The results indicate that formation of the anti-aggregatory adenosine and, to a lesser extent, breakdown of the pro-aggregatory ADP were decreased in diabetic lungs and may contribute to the platelet hyperreactivity associated with diabetes.
mesh_terms: D000241:Adenosine / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000378:metabolism*; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D008168:Lung / Q000098:blood supply / Q000378:metabolism*; D008297:Male; D011652:Pulmonary Circulation; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000241:Adenosine