title: Enzymatic determination of cholesterol in egg yolk.
abstract: The quantitative determination of cholesterol in egg yolk by using an enzymatic test kit is described. Cholesterol in the egg yolk is extracted with other lipid components by methylene chloride-methanol (2 + 1) and is enzymatically determined after saponification of the lipid extract. The method is relatively rapid, simple, and accurate and gives results which agree with those obtained by using a gas-liquid chromatographic (GLC) method. The mean cholesterol content of egg yolk determined by the enzymatic and GLC methods was 1237 and 1240 mg/100 g, respectively.
mesh_terms: D002789:Cholesterol Oxidase; D002791:Cholesterol, Dietary / Q000032:analysis*; D004530:Egg Yolk / Q000032:analysis*; D005260:Female; D013053:Spectrophotometry
chemical_list: D002791:Cholesterol, Dietary; D002789:Cholesterol Oxidase