Computer-based virus sensitivity assay and neutralization method. Applications for herpesviruses.
A method for computerized analysis of the antiviral efficiency of drugs and immunoglobulins was developed. The sensitivities of herpes simplex virus (HSV), cytomegalovirus (CMV) and varicella-zoster virus (VZV) to antiviral drugs were determined. A new type of neutralization assay could be performed rapidly and accurately with HSV and CMV. Combining immunological methods for herpesvirus antigen detection with a computerized analysis permitted rapid, sensitive measurement of drug sensitivities, comparison of antiviral activities at various virus concentrations and selection of potent neutralizing antisera.
D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis; D000998:Antiviral Agents / Q000494:pharmacology*; D003201:Computers; D004352:Drug Resistance, Microbial; D006564:Herpesviridae / Q000187:drug effects* / Q000276:immunology; D007136:Immunoglobulins / Q000276:immunology; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009500:Neutralization Tests / Q000379:methods*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000998:Antiviral Agents; D007136:Immunoglobulins