Vasoactive intestinal polypeptide: presence in neuroblastoma X glioma hybrid cells.
Extracts of neuroblastoma X glioma hybrid cells 108CC15 and their parental lines were investigated for the presence of vasoactive intestinal polypeptide (VIP). With the aid of a radioimmunoassay and a receptor binding assay, VIP activity was found in the hybrid cells, to a lesser extent in neuroblastoma cells, but not in glioma cells. These results suggest a neuronal function of VIP. Since the hybrid cells also contain acetylcholine and opioids, they may be useful in studies of co-release of neurohormones.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D005768:Gastrointestinal Hormones / Q000378:metabolism*; D005910:Glioma / Q000378:metabolism*; D006822:Hybrid Cells / Q000378:metabolism; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism*; D051381:Rats; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
D005768:Gastrointestinal Hormones; D014660:Vasoactive Intestinal Peptide