Detection of reversible and irreversible changes of erythrocyte osmotic fragility induced by Yoshida tumor cells in rats.
A coil planet centrifuge (CPC) is an apparatus for the dynamic measurement of erythrocyte osmotic fragility, and it was applied to the observation of altered erythrocyte osmotic fragility induced by Yoshida tumor cells. The osmotic fragility of erythrocytes directly collected from the vein passing through the site of tumor inoculation began to increase from day 4 post-inoculation, whereas the osmotic fragility of the erythrocytes collected from the vein through the tumor-free (normal) region of the same animal remained normal until day 8 post-inoculation. This discrepancy in osmotic fragility suggests the presence of a plasma factor which recovers the altered erythrocyte membrane, the hypothesis being strongly supported by the results of our in vitro experiments. On day 10 post-inoculation, osmotically fragile erythrocytes failed to respond to the plasma treatment in vitro, suggesting that an irreversible change had occurred on the erythrocyte membranes.
D000818:Animals; D002478:Cells, Cultured; D002498:Centrifugation; D006400:Hematocrit; D006461:Hemolysis; D008297:Male; D009996:Osmotic Fragility; D051381:Rats; D011919:Rats, Inbred Strains; D012517:Sarcoma, Yoshida / Q000097:blood* / Q000098:blood supply / Q000473:pathology; D014680:Veins