[Effect of biologically active substances on the alkaline phosphatase activity, viability and fertility of bull spermatozoa].
Studied was the effect of beta-glucuronidase, hydrocortisone, and mercaptoethanol in bull semen on the activity of the alkaline phosphatase and the viability and fertilizing capacity of spermatozoa. It was found that the beta-glucuronidase enzyme in conc. of 270 UI per cu. cm of semen enhanced the activity of one of the forms of AP in agar electrophoresis (AP-1) with the simultaneous enhancement of the thermal resistance of spermatozoa at 46 degrees C and their fertilizing capacity by 9.6 per cent at first insemination. At minimum concentration hydrocortisone (1 X 10(-6) M) lowered the heat resistance of spermatozoa at 39 degrees C. Mercaptoethanol was found to lower by 3 per cent the activity of semen alkaline phosphatase. It is suggested to use the beta-glucuronidase enzyme in the practice of artificial insemination out of all other biologically active agents studied.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D005298:Fertility / Q000187:drug effects*; D005966:Glucuronidase / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology; D008297:Male; D008623:Mercaptoethanol / Q000494:pharmacology; D013094:Spermatozoa / Q000187:drug effects* / Q000201:enzymology; D013696:Temperature
D008623:Mercaptoethanol; D000469:Alkaline Phosphatase; D005966:Glucuronidase; D006854:Hydrocortisone
Vliianie na niakoi biologichno aktivni veshtestva vŭrkhu aktivnostta na alkalnata fosfataza, zhiznesposobnostta i oploditelnata sposobnost na spermatozoidi ot bik.