RNase-sensitive glucocorticoid-receptor complexes from HELA cell nuclei.
Dexamethasone-receptor complexes can be extracted from HeLa cell nuclei by mild sonication. These complexes can account for 800-1000 binding sites/cell, and are indistinguishable from salt-extractable ones as judged by sucrose gradient centrifugation in the presence of 0.3 M KCl, showing a sedimentation coefficient of 3.6 S. These complexes, however, broadly sediment in the 7 to 3.6 S region of low salt sucrose gradients. Enzymatic treatment of soluble extracts from nuclear sonicates shows that RNA is associated to dexamethasone-receptor complexes.
D002467:Cell Nucleus / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism*
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; C028697:dexamethasone receptor; D012260:Ribonucleases