Selective reduction of phosphatidylglycerol and phosphatidylcholine in pulmonary surfactant by 4-aminopyrazolo(3,4d)pyrimidine in the rat.
Effects of 4-aminopyrazolo(3,4d)pyrimidine on pulmonary surface-active material and its surface activity were investigated in the rat. A rapid decrease in serum cholesterol was observed in rats treated with this drug and these effects continued during the entire period of treatment. Phosphatidylglycerol content in surface-active material and in the residual lung decreased significantly during three days of treatment and phosphtidylcholine content in surface-active material also decreased on the third day of the treatment. There were no changes in the contents of other phospholipids and cholesterol. The surface-active material from treated rats showed a larger surface compressibility, an elevated minimum surface tension, and a low stability index, as compared to control rats. These results show that alteration of lipid constituents significantly affects the surface properties of pulmonary surface-active material and that 4-aminopyrazolopyrimidine is a drug which can be effectively utilized to investigate lipid metabolism of the lung.
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D001835:Body Weight / Q000187:drug effects*; D002784:Cholesterol / Q000097:blood; D002855:Chromatography, Thin Layer; D005215:Fasting; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D010713:Phosphatidylcholines / Q000378:metabolism*; D010715:Phosphatidylglycerols / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis; D011663:Pulmonary Surfactants / Q000378:metabolism*; D051381:Rats; D013500:Surface Tension
D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010743:Phospholipids; D011663:Pulmonary Surfactants; C004420:4-aminopyrazolo(3,4-d)pyrimidine; D002784:Cholesterol; D000225:Adenine