Unusual SCD in cancer cells: a phenomenon related to decreased BrdU incorporation?
The simultaneous presence of chromosome segments with and without sister chromatid differentiation (SCD) in the same metaphase was observed in a human melanoma cell line after BrdU incorporation for 48-72 hr. This phenomenon was related to the time of BrdU incorporation by the cells: i.e., it was not observed after exposure to BrdU for only 17-20 hr. It is proposed, therefore, that the unusual staining pattern is caused through reduced BrdU incorporation by the cells after completing the first division.
D001973:Bromodeoxyuridine / Q000378:metabolism* / Q000633:toxicity; D002453:Cell Cycle; D002460:Cell Line; D002871:Chromosome Banding; D003434:Crossing Over, Genetic / Q000187:drug effects*; D006801:Humans; D007621:Karyotyping; D008545:Melanoma / Q000235:genetics*; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D001973:Bromodeoxyuridine