Simultaneous determination of disopyramide and its mono-N-dealkyl metabolite in plasma and urine by high-performance liquid chromatography.
A high-performance liquid chromatographic procedure is described for the determination of disopyramide and its mono-N-dealkyl metabolite which offers simplicity of extraction with excellent selectivity, sensitivity and reproducibility. The drug and metabolite, following basic diethyl ether extraction and back-extraction with acetic acid, are injected into a reversed-phase high-performance liquid chromatographic column and the absorbance of the eluate measured at 254 nm. Detectability limits of 0.05 micrograms/ml were obtained with both compounds, and studies of the reproducibility, precision, recovery, stability during storage and effect of time in separating plasma from erythrocytes are described. Applications of this high-performance liquid chromatographic procedure to plasma samples from patients on disopyramide therapy and to plasma and urine from a healthy dog administered single doses are reported.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004206:Disopyramide / Q000032:analysis* / Q000378:metabolism / Q000627:therapeutic use; D004285:Dogs; D006801:Humans; D007700:Kinetics; D011725:Pyridines / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D011725:Pyridines; D004206:Disopyramide