[Transitional states in a chemostat culture of Candida utilis].
Transient states of the chemostat Candida utilis 1668-3-37 culture were studied when its growth was limited by ethanol and an abrupt acidification of the medium from pH 5.0 to 2.2 was done or when the dilution rate was rapidly changed from D = 0.1 to 0.3 h-1 and back to 0.07 h-1. The pH shock was found to cause stronger oscillations in a number of parameters (the weight of dry biomass, the content of residual ethanol, the content of RNA in the cells) than a change in the dilution rate. In the latter case the population density changed more smoothly than the content of RNA did. DNA content remained at one and the same level in all of the experiments. All of the oscillations were observed only in the first generation after a shock; there upon, the culture remained for a long time (7 to 10 generations) in a very stable state typical of chemostat cultures. The oscillations induced by the unfavourable pH of the medium were compared with those caused by an abrupt change in the dilution rate. The pH shock brought about multiple damping oscillations of the parameters whereas a change in the dilution rate resulted, most often, in a merely one oscillation.
D002175:Candida / Q000502:physiology*; D004271:DNA, Fungal / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D012331:RNA, Fungal / Q000378:metabolism
D004271:DNA, Fungal; D012331:RNA, Fungal; D000431:Ethanol
Perekhodnye sostoianiia khemostatnoĭ kul'tury Candida utilis.