Amino acid sequence of the tryptic peptide containing the catalytic-site thiol group of bovine liver uridine diphosphate glucose dehydrogenase.
The catalytic-site thiol groups of UDP-glucose dehydrogenase from bovine liver were carboxymethylated with iodo[2-14C]acetate or with iodoacetamidofluorescein. After the residual thiol groups were carboxymethylated with iodoacetate, the proteins were digested with trypsin. The 14C-labelled peptide from the carboxymethylated enzyme was purified to homogeneity by successive thick-layer chromatography on silica gel, paper electrophoresis and chromatography, and column chromatography on Bio-Gel P-6. Homogeneous fluoresceincarboxamidomethylated peptide was prepared from a tryptic digest of fluoresceincarboxamidomethylated enzyme by specific adsorption--desorption from Sephadex G-25. The sequences of either peptide determined by the manual Edman dansyl procedure is: Ala-Ser-Val-Gly-Phe-Gly-Gly-Ser-Cys-Phe-Glx-Glx-Gly-Lys.
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D002384:Catalysis; D002417:Cattle; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008099:Liver / Q000201:enzymology*; D010446:Peptide Fragments / Q000302:isolation & purification*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism; D014533:Uridine Diphosphate Glucose Dehydrogenase / Q000378:metabolism*
D005452:Fluoresceins; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D013438:Sulfhydryl Compounds; C015296:5-iodoacetamidofluorescein; D002237:Carbohydrate Dehydrogenases; D014533:Uridine Diphosphate Glucose Dehydrogenase; D014357:Trypsin