The subcellular localization of the glycosphingolipids in the epithelial cells of rat small intestine.
Analysis of brush-border and basolateral membrane prepared from the epithelial cells of rat small intestine showed that the brush border contained less of some phospholipids and more glycosphingolipids per protein. The N-glycoloylneuraminosyllactosylceramide was enriched in the brush border, while cholesteryl sulphate and fucosyllactosylceramide were more prominent in the basolateral membrane. By periodate-NaB3H4 labelling of the intact small intestine, it was shown that N-glycoloylneuraminosyllactosylceramide is exposed at the luminal cell surface of the epithelial cell.
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002462:Cell Membrane / Q000032:analysis*; D004848:Epithelium / Q000032:analysis; D006028:Glycosphingolipids / Q000032:analysis*; D007421:Intestine, Small / Q000032:analysis*; D008297:Male; D008563:Membrane Lipids / Q000032:analysis*; D008871:Microvilli / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012794:Sialic Acids / Q000032:analysis
D006028:Glycosphingolipids; D008563:Membrane Lipids; D012794:Sialic Acids