Quantitation of Poly (A)-containing mRNA in rat cardiac biopsies.
Using a combination of conventional mRNA-extraction methods and newer hybridization techniques, a method was developed which allows the quantitation of cardiac Poly(A)-containing mRNA in cardiac biopsies. This provides a new tool to directly assess changes in cardiac gene expression in cardiac biopsies. It is proposed that the method described may be valuable in investigations dealing with various human or animal heart diseases where changes in cardiac gene expression are expected and where only biopsy material is available.
D000818:Animals; D001706:Biopsy; D005786:Gene Expression Regulation; D009206:Myocardium / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats
D012333:RNA, Messenger; D011061:Poly A