Fractionation of hagfish slime gland secretions: partial characterization of the mucous vesicle fraction.
This paper deals with the collection, fractionation and partial characterization of the slime gland secretion of the Pacific hagfish (Eptatretus stouti) with emphasis on the mucous fraction. Secretions were collected by electrical stimulation of the glands of anesthetized hagfish and, using three different methods, separated into three fractions: 1) the thread cells, 2) the mucous vesicles of the mucous cells, and 3) the soluble fraction. The methods take advantage of the stabilization of the thread cells and mucous vesicles by ammonium sulfate and sodium citrate.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005088:Exocrine Glands / Q000378:metabolism* / Q000648:ultrastructure; D005399:Fishes / Q000378:metabolism*; D006196:Hagfishes / Q000378:metabolism*; D008854:Microscopy, Electron; D009093:Mucus / Q000032:analysis*; D009391:Nephelometry and Turbidimetry; D011392:Proline / Q000032:analysis; D013447:Sulfites / Q000378:metabolism
D000596:Amino Acids; D002241:Carbohydrates; D013447:Sulfites; D011392:Proline