A high performance liquid chromatographic method of analysis of 4'-O-tetrahydropyranyladriamycin and their metabolites in biological samples.
A method for measuring 4'-O-tetrahydropyranyladriamycin (THP) and its metabolites in biological samples are described. By reversed-phase high performance liquid chromatography using fluorescence detection, THP and its metabolites were all separated on a single chromatogram within 18 minutes. A linear calibration curve was obtained up to 2,000 ng/ml of THP in plasma. The recovery of THP in the analysis was more than 95% above 5 ng/ml and 87.1% even at 1.25 ng/ml. Thus the lower limit was 1.25 ng/ml in biological samples. Blood levels and urinary excretion in mice and dogs were satisfactory measured by this analytical method.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism / Q000652:urine; D007202:Indicators and Reagents; D007700:Kinetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains
D007202:Indicators and Reagents; D004317:Doxorubicin; C027260:pirarubicin