title: Ultrastructural and functional development of macrophages in the dermal tissue of rat fetuses.
abstract: After about 12 days of gestation, fetal macrophages begin to appear in the subepidermal mesenchyme of rat fetuses. The macrophages are ultrastructurally characterized by cytoplasmic vacuoles, abundant polyribosomes and long filopodia. Immunocytologically, they possess Fc and complement (C3) receptors on the cell surface and are capable of immune phagocytosis, Latex or carbon phagocytosis, and glass adherence. From 15 days of gestation, lysosomal granules and micropinocytic vesicles gradually develop, together with an enlargement of Golgi complexes, whereas the number of polysomes and the number and size of cytoplasmic vacuoles are gradually reduced when gestation ends. Finally, the macrophages become amoeboid. Non-specific esterase and endogenous peroxidase activities are always absent in these macrophages. In culture experiments with cell suspensions prepared from the mesenchyme, fetal macrophages show a similar maturation process. Autoradiography with 3H-thymidine demonstrates a high proliferative capacity of the macrophages, particularly during the fetal stage.
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002470:Cell Survival; D005260:Female; D005865:Gestational Age; D008264:Macrophages / Q000166:cytology / Q000276:immunology / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D009940:Organoids / Q000648:ultrastructure; D010587:Phagocytosis; D051381:Rats; D012397:Rosette Formation; D012867:Skin / Q000166:cytology / Q000196:embryology*; D014617:Vacuoles / Q000648:ultrastructure