The application of a potential-sensitive cyanine dye to rat small intestinal brush border membrane vesicles.
The sensitivity of the fluorescent dye, 3,3'-diethylthiadicarbocyanine (DiS-C2(5], was too low for the detection of membrane potential changes in rat small intestinal membrane vesicles. Only after adding LaCl3 or after fractionation of the intestinal membranes by free-flow electrophoresis could the dye be used to monitor electrogenic Na+-dependent transport systems. It is concluded that the response of this potential-sensitive dye is influenced by the negative surface charge density of the vesicles.
D000818:Animals; D052160:Benzothiazoles; D002232:Carbocyanines; D002462:Cell Membrane / Q000502:physiology*; D005456:Fluorescent Dyes; D007421:Intestine, Small / Q000502:physiology* / Q000648:ultrastructure; D007583:Jejunum / Q000502:physiology; D007687:Kidney Tubules, Proximal / Q000502:physiology*; D007700:Kinetics; D008297:Male; D008564:Membrane Potentials; D008871:Microvilli / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013844:Thiazoles
D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D013844:Thiazoles; C025905:3,3'-diethylthiatricarbocyanine