A homogeneous sol particle immunoassay for total oestrogens in urine and serum samples.
Conjugates prepared by adsorption of antibodies to colloidal gold particles were used in a homogeneous sol particle immunoassay for total oestrogens. The assay is based on inhibition by free oestrogens in the sample of the agglutination of a reaction mixture, consisting of the sample, the gold particle anti-oestriol conjugate and a suitable amount of oestriol-16/17-monosuccinyl-bovine serum albumin. The effect of different assay conditions is discussed. Under optimum conditions for an assay performed at room temperature the measuring range for E3 in buffer was 2-10 ng/ml. After appropriate pretreatment of samples the assay can be used to quantitate the level of total oestrogens in both urine and serum. The within-run coefficient of variation was 6.3% and over-all 6.9%. This method is also suitable for the assay of other haptens.
D003102:Colloids; D004306:Dose-Response Relationship, Immunologic; D004967:Estrogens / Q000032:analysis* / Q000097:blood / Q000652:urine; D005260:Female; D006046:Gold; D006241:Haptens / Q000032:analysis; D006801:Humans; D007118:Immunoassay / Q000379:methods; D012710:Serum Albumin, Bovine / Q000276:immunology; D013696:Temperature
D003102:Colloids; D004967:Estrogens; D006241:Haptens; D012710:Serum Albumin, Bovine; D006046:Gold