Isoform specificity of monoclonal hybridoma antibodies to quail skeletal muscle myosin subunits.
Monoclonal antibodies to adult quail breast muscle myosin (QBM) have been prepared and characterized using a solid phase enzyme linked immunosorbent assay and immunoblot procedures. One antibody (QBM-1) is directed against an epitope in the rod portion of the myosin heavy chain while another (QBM-2) binds exclusively to a conserved portion of the two alkali light chains of fast muscle myosin. Both of these antibodies cross-react with myosin from myotubes cultured in vitro but do not recognize non-muscle myosin. The application of these antibodies to the study of myogenesis is discussed.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D007120:Immunochemistry; D009132:Muscles / Q000378:metabolism*; D009218:Myosins / Q000276:immunology / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000276:immunology; D011485:Protein Binding; D011784:Quail
D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; D009218:Myosins