Purification and partial characterization of a daunorubicin-binding protein from rat liver.
A daunorubicin-binding protein has been isolated from the soluble fraction of normal rat liver by affinity chromatography on daunorubicin-Sepharose columns. This protein, which apparently binds anthracyclines specifically, appears to be composed of four polypeptides of 54,000 Mr, is different from tubulin, and is not involved in anthracycline metabolism. Its physiological function remains to be determined.
D000444:Aldehyde Dehydrogenase; D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification*; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D003630:Daunorubicin / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D012995:Solubility
D002352:Carrier Proteins; C039213:daunorubicin-binding protein, rat; D000444:Aldehyde Dehydrogenase; D003630:Daunorubicin