An improved technique for separation of neutral glycosphingolipids by high-performance liquid chromatography.
We have developed a high-performance liquid chromatographic (HPLC) procedure for separation of O-acetyl-N-p-nitrobenzoyl derivatives of six neutral glycosphingolipids: glucosylceramide, lactosylceramide, globotriaosylceramide, lactotriaosylceramide, globotetraosylceramide, and neolactotetraosylceramide. The recoveries of glucosylceramide and globotetraosylceramide for the derivatization procedure and HPLC analysis were approximately 75%, and one nanomole of glycolipid could be detected. The procedure was used for analysis of human erythrocyte neutral glycolipids.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004912:Erythrocytes / Q000032:analysis; D005915:Globosides / Q000302:isolation & purification; D005963:Glucosylceramides / Q000302:isolation & purification; D006028:Glycosphingolipids / Q000097:blood / Q000302:isolation & purification*; D006801:Humans; D007790:Lactosylceramides / Q000302:isolation & purification; D014281:Trihexosylceramides
D005915:Globosides; D005963:Glucosylceramides; D006028:Glycosphingolipids; D007790:Lactosylceramides; D014281:Trihexosylceramides; C024032:globotetraosylceramide; C018549:globotriaosylceramide; C025575:lactotriaosylceramide