title: Identification of 5 alpha-dihydrotestosterone in avian plasma.
abstract: Conscious adult domestic mallard drakes were intravenously injected with tritiated testosterone. After 30 min of in vivo conversion the steroids were extracted from the plasma and submitted to thin-layer chromatography (TLC). Using radiogas chromatography, labeled 5 alpha-dihydrotestosterone (5 alpha-DHT), androsterone, and epiandrosterone could be identified in the androstanolone fraction. 5 alpha-DHT was separated in a second TLC system and definitively identified by recrystallization to constant specific activity.
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D013196:Dihydrotestosterone / Q000097:blood*; D004372:Ducks / Q000097:blood*; D012621:Seasons
chemical_list: D013196:Dihydrotestosterone