Effect of psychotrophic drugs on [14C]tryptophan metabolism in cultured rat pineal gland.
A potentiated production of [14C]melatonin ([14C]Mt) with a simultaneous decline in [14C]serotonin ([14C]5-HT) conversion to its final metabolite [14C]5-hydroxyindole acetic acid ([14C]5-HIAA) was recorded in a rat pineal medium cultured with [14C]tryptophan and either a neuroleptic [haloperidol (HAL) or chloropromazine (CPZ)] or an antidepressant [desipramine (DIP)] drug. A difference between the pharmacological groups of drugs was evident only in [14C]5-HT levels, which were found significantly elevated in the presence of the neuroleptic HAL and reduced in the presence of the tricyclic antidepressant DIP. A parallel trend was observed in norepinephrine (NE)-induced [14C]tryptophan metabolism in the pineal culture medium. All three drugs tested brought about further enhancement of NE-induced synthesis of MT; HAL produced an elevated, but DIP a reduced, level of [14C]5-HT, compared to the NE-induced value.
D000818:Animals; D002478:Cells, Cultured; D002746:Chlorpromazine / Q000494:pharmacology; D003891:Desipramine / Q000494:pharmacology; D006220:Haloperidol / Q000494:pharmacology; D008297:Male; D008550:Melatonin / Q000378:metabolism; D009638:Norepinephrine / Q000494:pharmacology; D010640:Phenothiazines / Q000494:pharmacology; D010870:Pineal Gland / Q000378:metabolism*; D011619:Psychotropic Drugs / Q000494:pharmacology*; D051381:Rats; D012701:Serotonin / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism*
D010640:Phenothiazines; D011619:Psychotropic Drugs; D012701:Serotonin; D014364:Tryptophan; C031637:phenothiazine; D006220:Haloperidol; D008550:Melatonin; D003891:Desipramine; D002746:Chlorpromazine; D009638:Norepinephrine