Stimulation of 1,25-dihydroxycholecalciferol production by prolactin and related peptides in intact renal cell preparations in vitro.
Ovine prolactin stimulated the 1 alpha-hydroxylase activity in isolated renal tubules and especially in primary kidney cell cultures, both prepared from vitamin D-deficient chicks. In primary chick kidney cell cultures, treated for 48 h with 1,25-dihydroxycholecalciferol (to induce the 24-hydroxylase activity) ovine prolactin, after a 1 h incubation period, stimulated the 1 alpha-hydroxylase activity without affecting the 24-hydroxylase activity. Similar results were obtained with related peptide hormones such as human growth hormone, chicken growth hormone, and human placental lactogen. These observations are discussed in relation to the possible role of these peptide hormones as modulators of 1,25-dihydroxycholecalciferol production in physiological situations of calcium stress, such as pregnancy, lactation, growth in mammals and egg laying in birds.
D000818:Animals; D002117:Calcitriol; D002478:Cells, Cultured; D002645:Chickens; D004100:Dihydroxycholecalciferols / Q000096:biosynthesis*; D004790:Enzyme Induction; D013006:Growth Hormone / Q000502:physiology*; D006801:Humans; D006887:Hydroxycholecalciferols / Q000096:biosynthesis*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D006899:Mixed Function Oxygenases / Q000096:biosynthesis; D010928:Placental Lactogen / Q000502:physiology*; D011388:Prolactin / Q000502:physiology*; D012756:Sheep
D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols; D011388:Prolactin; D013006:Growth Hormone; D010928:Placental Lactogen; D006899:Mixed Function Oxygenases; D002117:Calcitriol