DNA binding proteins in chromatin structure and function.
The binding of chromosomal proteins to cloned mouse and human globin genes can be determined by transferring proteins from one dimensional electrophoretic separations on to nitrocellulose paper and exposing them to nick translated cloned globin DNAs. The usefulness of this technique is assessed in the case of RNA polymerases isolated from mouse Ascites tumor cells where a specific initiation site for transcription of mouse beta globin DNA can be demonstrated.
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002843:Chromatin / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004274:DNA, Recombinant / Q000378:metabolism; D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D005914:Globins / Q000235:genetics*; D006801:Humans; D051379:Mice; D014158:Transcription, Genetic
D002352:Carrier Proteins; D002843:Chromatin; D004274:DNA, Recombinant; D004268:DNA-Binding Proteins; D005914:Globins; D004247:DNA; D012321:DNA-Directed RNA Polymerases