Inhibition of platelet aggregation by primary amines. Evidence for a possible role of membrane-associated calcium.
The aggregation of human blood platelets by thrombin, adenosine diphosphate, wheat germ agglutinin or ristocetin was inhibited by primary amines. In general, thrombin-induced platelet aggregation was strongly affected by the amines while the effect was weak on cell aggregation by ristocetin. Usually, the diamines were stronger inhibitors of aggregation than the monoamines with cadaverine as the strongest and ethylamine as the weakest inhibitor. At concentration where platelet aggregation was inhibited, the amines neither displaced serotonin from serotonin-loaded platelets nor caused lysis of human red cells. The lectin activity of wheat germ agglutinin on human red cells was not affected by the amines indicating that the amines probably acted on platelets and not on the agglutinin. The clotting activity of thrombin on fibrinogen was partially inhibited by the amines while its esterolytic activity remained unaltered. The inhibitory action of the amines on platelet aggregation could be overcome with small amounts of calcium while other divalent cations tested had little effect. It is suggested that the amines affect platelet aggregation by interfering with the actions of membrane-associated calcium.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000588:Amines / Q000494:pharmacology*; D002103:Cadaverine / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D005021:Ethylamines / Q000494:pharmacology; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D012310:Ristocetin / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology; D014909:Wheat Germ Agglutinins
D000588:Amines; D005021:Ethylamines; D037102:Lectins; D014909:Wheat Germ Agglutinins; D012310:Ristocetin; D000244:Adenosine Diphosphate; D013917:Thrombin; D002103:Cadaverine; D002118:Calcium