Reversed-phase high-performance liquid chromatographic separation of saccharin, caffeine and benzoic acid using non-linear programming.
A method is described which allows a chromatographic assay to be optimized with respect to the time needed to complete an analysis, while maintaining good resolution. The method introduces an operational research technique called linear programming. It was used to optimize the eluent composition for the assay of saccharin, caffeine and benzoic acid and resulted in a significant reduction in the analysis time.
D001565:Benzoates / Q000302:isolation & purification*; D019817:Benzoic Acid; D001628:Beverages / Q000032:analysis; D002110:Caffeine / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008956:Models, Chemical; D012439:Saccharin / Q000302:isolation & purification*; D012984:Software
D001565:Benzoates; D002110:Caffeine; D019817:Benzoic Acid; D012439:Saccharin