Membrane damage of liposomes by the mushroom toxin phallolysin.
We have investigated the membrane-damaging effect of phallolysin on liposomes varying in phospholipid composition, net charge and physical constitution. Liposomes were prepared from lipids extracted from bovine or human erythrocyte ghosts. The liposomes composed of bovine lipids (the intact cell showing little sensitivity to phallolysin) were found comparably sensitive to those prepared from lipids of human red cells (these cells being of high sensitivity). In addition, artificial mixtures of lipids were used for the preparation of liposomes, consisting of (a) negatively charged phospholipids such as dicetyl phosphate or phosphatidylserine, (b) cholesterol, and (c) either sphingomyelin (as the major component of erythrocytes from ruminants) or phosphatidylcholine (as the major component of erythrocytes from non-ruminants). Again, we found only little difference in the susceptibilities of sphingomyelin- and phosphatidylcholine-containing liposomes. On the other hand, the susceptibility depended on the presence of phospholipids with negative net charges. Omittance of phosphatidylcholine or dicetyl phosphate, or replacement by the positively charged stearylamine, decreased the susceptibility by a factor of more than 20. Finally, we prepared liposomes from dicetyl phosphate, cholesterol and phosphatidylcholine in two physical states: large unilamellar and smaller multilamellar liposomes. The unilamellar liposomes were about 10-times more sensitive to phallolysin. We conclude: (1) Phallolysin damages phospholipid-membranes in the absence of receptor proteins, but high concentrations of the toxin are required. (2) Membrane damage takes place with liposomes containing phosphatidylcholine as well as those containing sphingomyelin. (3) Phallolysin damages only liposomes containing phospholipids with a negative net charge.
D000546:Amanitins / Q000633:toxicity*; D000818:Animals; D002417:Cattle; D003603:Cytotoxins / Q000633:toxicity*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D007700:Kinetics; D008081:Liposomes; D008563:Membrane Lipids / Q000097:blood; D008854:Microscopy, Electron; D008954:Models, Biological; D010743:Phospholipids / Q000097:blood; D013329:Structure-Activity Relationship
D000546:Amanitins; D003603:Cytotoxins; D008081:Liposomes; D008563:Membrane Lipids; D010743:Phospholipids; C008824:phallolysine