A radioimmunoassay for 6-keto-prostaglandin F1 alpha.
We describe a highly specific RIA for blood and urine 6-keto-PGF1 alpha, the principal metabolite of PGI2. The method requires extraction and column chromatography prior to assay and uses a tritium trace for assay monitoring. Dextran-coated charcoal is used to separate bound from free trace. Acceptable standard curve reproducibility over six assays had a mean correlation of 0.991 with a coefficient of variation of 6.61%. Good precision and accuracy data were generated with this assay, and normal human blood levels in 80 control subjects was less than 125 pg/ml. Significant elevations of blood 6-keto-PGF1 alpha were found in a high proportion of cancer patients with various solid tumor malignancies.
D015121:6-Ketoprostaglandin F1 alpha; D000818:Animals; D000906:Antibodies; D003429:Cross Reactions; D006801:Humans; D011460:Prostaglandins F / Q000097:blood*; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods
D000906:Antibodies; D011460:Prostaglandins F; D015121:6-Ketoprostaglandin F1 alpha