The effect of dichloroacetate and hydroxypyruvate on the entry of 14C from [1-14C]alanine into urea in rat hepatocytes.
Entry of metabolic 14CO2 into urea is shown to be decreased by dichloroacetate although the production of 14CO2 is stimulated 2-fold. Hydroxypyruvate, a product of dichloroacetate metabolism, increases the incorporation of metabolic 14CO2 into urea. It is proposed that these effects result from changes in the cytoplasmic-mitochondrial pH gradient.
D000085:Acetates / Q000494:pharmacology*; D000409:Alanine / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D003999:Dichloroacetic Acid / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D011773:Pyruvates / Q000494:pharmacology*; D051381:Rats; D014508:Urea / Q000378:metabolism*
D000085:Acetates; D002250:Carbon Radioisotopes; D011773:Pyruvates; D014508:Urea; C012375:hydroxypyruvic acid; D003999:Dichloroacetic Acid; D000409:Alanine