[Effect of ethylmethane sulfonate on the expression of one of the traits of malignancy by cultured mouse cells].
The influence of ethyl methane sulfonate (methane sulfonic acid ethyl esther, EMS) on anchorage independence of tumor cells was studied. Mouse near-diploid spontaneously transformed clonal fibroblasts (CAK-25Agr were used. They were characterized by a stable low cloning efficiency in 1,2% methyl cellulose ((3-5) . 10(-5) per cell seeded into a semi-solid medium). EMS enhanced the quantity of CAK-25Agr colonies grown in methyl cellulose. However, this enhancement was only obtained when correction on the cloning efficiency of the cells in a liquid medium was introduced. Subclones of CAK-25Agr isolated from methyl cellulose were studied for their ability to form colonies in the semi-solid medium. The number of subclones with elevated anchorage independence in cultures treated by a mutagen and in untreated cultures did not differ.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002478:Cells, Cultured; D002999:Clone Cells / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D005020:Ethyl Methanesulfonate / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D008747:Methylcellulose / Q000494:pharmacology; D051379:Mice; D013997:Time Factors
D003470:Culture Media; D008747:Methylcellulose; D005020:Ethyl Methanesulfonate
Vliianie étilmetansul'fonata na ékspressiiu odnogo iz priznakov malignizatsii kul'tiviruemymi kletkami myshi.