Protein synthesis in cells infected with Semliki Forest virus is not controlled by intracellular cation changes.
Treatment of BHK cells with 1 microM nigericin results in a 55% decrease in K+ and a 3.3-fold increase in intracellular Na+; protein synthesis under these conditions is depressed by 35%. In BHK cells infected with Semliki Forest virus (SFV), protein synthesis is depressed by 76% 6.5 h after infection; intracellular K+ is unchanged, and intracellular Na+ is increased 1.8-fold at this time. These results suggest that the increase in intracellular Na+ in SFV-infected BHK cells does not adequately account for the decrease in protein synthesis, and makes it likely that an increased Na+ concentration is a consequence, not a cause, of alterations in protein synthesis in virally-infected cells. No evidence was obtained for the purported [Alonso, M. A. and Carrasco, L. (1980) Eur. J. Biochem. 109, 535-540; (1981) Eur. J. Biochem. 118, 289-294; (1981) FEBS Lett. 127, 112-114] ability of 1 microM nigericin to permeabilize' cells.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D006224:Cricetinae; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D051379:Mice; D009550:Nigericin / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism*; D012672:Semliki forest virus / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis*; D014776:Virus Cultivation
D000900:Anti-Bacterial Agents; D014764:Viral Proteins; C011265:guanosine 5'-(beta,gamma-methylene)triphosphate; D006160:Guanosine Triphosphate; D012964:Sodium; D009550:Nigericin; D011188:Potassium