title: Distribution of nucleosomes on reconstituted chromatin from cloned mouse beta-globin DNA.
abstract: Relative abundance of nucleosomes on reconstituted chromatin was estimated with cloned mouse beta-globin gene DNA. Mononucleosomal DNA was isolated from reconstituted chromatin after digestion with micrococcal nuclease, nick-translated and used as a probe for blot hybridization. DNA fragments of restriction nuclease-digested globin DNA were transferred to DBM-paper and hybridized with mononucleosomal [32P] DNA probe. The results showed non-random distribution of nucleosomes.
mesh_terms: D000818:Animals; D002843:Chromatin / Q000032:analysis*; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D005914:Globins / Q000235:genetics*; D051379:Mice; D009707:Nucleosomes / Q000032:analysis*; D014018:Tissue Distribution
chemical_list: D002843:Chromatin; D009707:Nucleosomes; D005914:Globins; D004247:DNA