[Hydrolysis of cellulose by fungi. II. Production of cellulases by Trichoderma harzianum by fermentation in liquid media].
Microcristalline cellulose (cellulose Avicel, Merck) supported growth of Trichoderma harzianum and induced production of cellulases in liquid cultures. After 50 h growth, the total cellulasic activities present in both the supernatant and the mycelium were 3,000 IU/l of carboxymethyl cellulose, 400 IU/l of filter paper activity, and 4 IU/l of cotton activity corresponding to 1.7 g/l of proteins. Cellulase production could be increased by a preliminary treatment of cellulose, and pH regulation during growth. The influence of inoculum concentration was studied and an optimum of 3 x 10(7) conidia/g dry weight of substrate was demonstrated. Using a synthetic culture medium, a soluble factor of germination was demonstrated which could be leached out by 3 successive washings of conidia.
D002480:Cellulase / Q000096:biosynthesis*; D002482:Cellulose / Q000378:metabolism; D003470:Culture Media; D005656:Fungal Proteins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D003904:Mitosporic Fungi / Q000201:enzymology*; D013172:Spores, Fungal / Q000502:physiology; D014242:Trichoderma / Q000201:enzymology* / Q000502:physiology
D003470:Culture Media; D005656:Fungal Proteins; D002482:Cellulose; D002480:Cellulase
Hydrolyse de la cellulose par les moisissures. II. Production de cellulases de Trichoderma harzianum par fermentation en milieu liquide.