[Cytotoxic effects of carbonyl compounds (4-hydroxyalkenals) derived from peroxidation of hepatic microsomal lipids on isolated hepatocytes].
Carbonyl compounds released during the NADPH-Fe dependent lipid peroxidation and identified as 4-hydroxyalkenals (almost entirely as 4-hydroxynonenal), when incubated with isolated hepatocytes, produce loss of viability in 95% of the cells, as measured by the trypan blue exclusion test. They also produce an almost complete permeabilization of the plasma-membrane, as measured by the test of the permeability to NADH. concomitantly with the permeabilization of the plasma-membrane, a marked release of enzymes (lactate dehydrogenase and glutamate-pyruvate transaminase) from the hepatocytes occurs. These and other activities of the above mentioned carbonyl compounds suggest the possibility that these products represent some of the effective mediators of the liver injury produced by those toxins, such as CCL4, which promote the peroxidation of membrane lipids.
D000410:Alanine Transaminase / Q000378:metabolism; D000447:Aldehydes / Q000494:pharmacology; D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D003603:Cytotoxins / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008054:Lipid Peroxides / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects*; D008297:Male; D008862:Microsomes, Liver / Q000032:analysis*; D009243:NAD / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000447:Aldehydes; D003603:Cytotoxins; D008054:Lipid Peroxides; D009243:NAD; D007770:L-Lactate Dehydrogenase; D000410:Alanine Transaminase; C027576:4-hydroxy-2-nonenal
Effetto citotossico di composti carbonilici (4-idrossialchenali) derivati dalla perossidazione dei lipidi microsomali epatici sugli epatociti isolati.