title: Glycoprotein-like large glucagon immunoreactive species in extracts of the fetal bovine pancreas.
abstract: Large glucagon immunoreactive substances, extracted from the fetal bovine pancreas and separated by gel filtration in the presence of 6 M guanidinium-hydrochloride, were submitted to lectin-sepharose affinity column chromatograph. Gel-filtered peak I (approximately 45 K delta) and peak II (approximately 10 K delta) interacted biospecifically with concanavalin-A- and wheat-germ-lectin-sepharoses, suggesting glycoproteins as possible constituents of large glucagon immunoreactive substances in extracts of the fetal bovine pancreas. The glucagon-like immunochemical identity of the lectin-sepharose-bound substances was further substantiated by binding to antiglucagon antibodies-sepharose and by characteristic proportional dilutions in the glucagon radioimmunoassay.
mesh_terms: D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D005333:Fetus / Q000378:metabolism; D005934:Glucagon / Q000276:immunology / Q000302:isolation & purification*; D004763:Glucagon-Like Peptides; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification*; D010179:Pancreas / Q000378:metabolism*; D010455:Peptides; D011863:Radioimmunoassay
chemical_list: D006023:Glycoproteins; D010455:Peptides; D004763:Glucagon-Like Peptides; C015236:glucagon-like-immunoreactivity; D005934:Glucagon