title: Studies on structural changes of F-actin and myosin in living, intact and damaged muscle fibres by means of polarized ultraviolet fluorescence microscopy.
abstract: By means of polarized ultraviolet fluorescence microscopy the structural changes of F-actin and myosin were discovered at the changing a functional state of a living muscle fibre and during spreading degeneration (Zenker's necrosis). The character of conformational changes of F-actin and myosin at activation, contraction, contracture and rigor is similar, but the number of changed macromolecules depends on a fibre state. At fibre local damage in its morphological intact parts there was found an alternation of zones, reflecting two states unusual for a fibre. During spreading degeneration these states transform into irreversible contracture and then into rigor. Similar changes were observed in muscle fibres obtained from denervated muscles.
mesh_terms: D000199:Actins / Q000032:analysis*; D000818:Animals; D001001:Anura; D005454:Fluorescence Polarization; D008856:Microscopy, Fluorescence / Q000379:methods; D009119:Muscle Contraction; D009126:Muscle Relaxation; D009127:Muscle Rigidity; D009132:Muscles / Q000032:analysis / Q000166:cytology* / Q000473:pathology; D009218:Myosins / Q000032:analysis*; D009336:Necrosis; D014466:Ultraviolet Rays
chemical_list: D000199:Actins; D009218:Myosins