Relative proportions of human hemoglobins A, S, C in individual erythrocytes of heterozygotes.
Inherited electrophoretic variants of hemoglobin in individual erythrocytes were separated by electrophoresis in ultrathin acid agar gels. The electropherograms were stained under a hood with hemoglobin specific chromogen, benzidine. Photomicrographs of the electropherograms were scanned with a recording densitometer. Relative quantities of the respective hemoglobins in individual erythrocytes were estimated by weighing the areas under the densitograph curves. The findings confirm that the intracellular proportions of Hb A and Hb S or Hb A and Hb C in heterozygotes are of heterogeneous distributions within a given population of cells. The tool of single cell electrophoresis may be useful for investigating relative quantities of other electrophoretically separable protein variants in individual erythrocytes.
D000755:Anemia, Sickle Cell / Q000097:blood / Q000235:genetics; D001560:Benzidines; D001797:Blood Protein Electrophoresis; D003720:Densitometry; D004912:Erythrocytes / Q000032:analysis*; D006441:Hemoglobin A / Q000032:analysis*; D006444:Hemoglobin C / Q000032:analysis*; D006451:Hemoglobin, Sickle / Q000032:analysis*; D006579:Heterozygote; D006801:Humans; D010641:Phenotype
D001560:Benzidines; D006451:Hemoglobin, Sickle; C029876:benzidine; D006444:Hemoglobin C; D006441:Hemoglobin A