On the substrate specificity of a damage-specific DNA binding protein from human cells.
The nature of the lesion recognized by a damage-specific DNA binding protein from human cells was investigated by examining the substrate specificity of this protein. Protein-recognizable damage was introduced into both T7 DNA and Poly d(A-T) at relatively low UV fluences. In addition, the protein demonstrated binding to both nitrous acid- and bisulfite-treated DNA, but not to DNA crosslinked with trioxsalen plus near-UV nor to non-irradiated uracil-containing DNA. These results suggest that this protein could be recognizing minor helix distortions in DNA rather than any one single lesion.
D002352:Carrier Proteins / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004260:DNA Repair; D004279:DNA, Viral / Q000528:radiation effects; D004268:DNA-Binding Proteins; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D009608:Nitrous Acid; D009690:Nucleic Acid Conformation; D010920:Placenta / Q000378:metabolism; D011067:Poly dA-dT / Q000528:radiation effects; D011247:Pregnancy; D013447:Sulfites; D014307:Trioxsalen; D014466:Ultraviolet Rays; D014498:Uracil
D002352:Carrier Proteins; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D013447:Sulfites; D011067:Poly dA-dT; D014498:Uracil; D004247:DNA; D009608:Nitrous Acid; C009279:sodium bisulfite; D014307:Trioxsalen