Fluorimetric assays for cathepsin B and cathepsin H with methylcoumarylamide substrates.
Benzyloxycarbonyl-phenylalanyl-arginine 4-methyl-7-coumarylamide was found to be an excellent substrate for the fluorimetric assay of cathepsin B, and arginine 4-methyl-7-coumarylamide for cathepsin H. Procedures were developed that are very convenient, and avoid the hazards associated with the use of naphthylamides.
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives*; D002401:Cathepsin B; D056655:Cathepsin H; D002403:Cathepsins / Q000378:metabolism*; D003374:Coumarins; D003546:Cysteine Endopeptidases; D004151:Dipeptides; D006801:Humans; D008247:Lysosomes / Q000201:enzymology; D051381:Rats; D013050:Spectrometry, Fluorescence / Q000379:methods; D013379:Substrate Specificity
D003374:Coumarins; D004151:Dipeptides; C021159:benzyloxycarbonyl-phenylalanylarginine-4-methylcoumaryl-7-amide; C041841:arginine 4-methyl-7-coumarylamide; D001120:Arginine; D002403:Cathepsins; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; C534126:CTSH protein, human; D056655:Cathepsin H; C534128:Ctsh protein, rat