Improvement of the parallel flow dialysis technique for the detection of drug-binding proteins in column effluents.
The parallel flow dialysis technique was improved for application to the detection of drug-binding proteins in a column chromatographic effluent. To prevent the baseline drift, the pressures of both protein and drug channels were maintained equal during chromatography, and Brij-35 was added to the solvents. The improved method was successfully applied to the detection of methyl orange-binding proteins in human serum and bromphenol blue-binding proteins in rat liver homogenate.
D000818:Animals; D001391:Azo Compounds / Q000378:metabolism; D001978:Bromphenol Blue / Q000378:metabolism; D003956:Dialysis / Q000379:methods; D006801:Humans; D008099:Liver / Q000032:analysis / Q000378:metabolism; D051381:Rats; D011955:Receptors, Drug / Q000032:analysis / Q000097:blood*
D001391:Azo Compounds; D011955:Receptors, Drug; D001978:Bromphenol Blue; C100258:methyl orange