Cholinergic receptor binding and autoradiography in brains of non-neurological and senile dementia of Alzheimer-type patients.
Muscarinic and nicotinic cholinergic receptor distribution was studied by dry-mount autoradiography in brains obtained postmortem from patients with senile dementia of Alzheimer-type (SDAT) and non-neurological controls. Sections were incubated with either [N-methyl-3H]scopolamine, ([3H]NMS) or [125I]alpha-bungarotoxin, ([125I]alpha-BTX). No significant difference in the affinity and number of muscarinic and nicotinic receptors was found in hippocampus, frontal, temporal and cingulate cortex between SDAT patients and non-neurological controls. However, some SDAT cases showed diffuse instead of laminar [3H]NMS labeling in cortical regions. The labeling pattern was not affected by the presence of neuritic plaques and neurofibrillary tangles.
D000368:Aged; D000544:Alzheimer Disease / Q000378:metabolism*; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D002038:Bungarotoxins / Q000378:metabolism; D003704:Dementia / Q000378:metabolism*; D005625:Frontal Lobe / Q000378:metabolism; D006179:Gyrus Cinguli / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D019832:N-Methylscopolamine; D011950:Receptors, Cholinergic / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000378:metabolism; D012602:Scopolamine Derivatives / Q000378:metabolism; D013702:Temporal Lobe / Q000378:metabolism
D002038:Bungarotoxins; D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D011978:Receptors, Nicotinic; D012602:Scopolamine Derivatives; D019832:N-Methylscopolamine