Presence of fucosyl residues on the oligosaccharide antennae of membrane glycopeptides of human neuroblastoma cells.
Fucosyl residues linked alpha 1 leads to 3 or 4 to N-acetylglucosamine were found in large amounts on glycopeptides from the membranes of human tumor cells of neurectodermal origin but not on membrane glycopeptides from human fibroblasts. The fucosyl residues were detected by release of radioactive fucose from the glycopeptides with an almond alpha-L-fucosidase specific for fucosyl alpha 1 leads to 3(4)-N-acetylglucosamine. In other studies, the linkage was shown to be alpha 1 leads to 3 by nuclear magnetic resonance analysis (U. V. Santer, M. C. Glick, H. van Halbeek, and J. F. G. Vliegenthart. Carbohydr. Res., 118: in press, 1983). Glycopeptides containing these fucosyl residues from four human neuroblastoma cell lines were defined by binding to immobilized lectins. In addition, the glycopeptides from one human neuroblastoma cell line, CHP-134, were further characterized by enzyme degradation and columns calibrated for size and charge. The antennary position of fucosyl alpha 1 leads to 3-N-acetylglucosamine on the glycopeptides was demonstrated by the use of exoglycosidases and endoglycosidase D, since complete degradation to yield fucosyl-N-acetylglucosaminylasparagine was obtained only after treatment with almond alpha-L-fucosidase prior to the sequential degradation. Fucosyl alpha 1 leads to 3-N-acetylglucosamine was present on most size and charge classes of membrane glycopeptides and therefore was not limited to a few glycoproteins. Since the almond alpha-L-fucosidase cleaves fucosyl residues from glycoproteins, the physiological effects of the increased specific fucosylation on human tumors of neurectodermal origin can be examined.
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002250:Carbon Radioisotopes; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis; D005643:Fucose / Q000032:analysis*; D006020:Glycopeptides / Q000032:analysis*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis*; D009369:Neoplasms / Q000032:analysis; D009447:Neuroblastoma / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis*; D014316:Tritium
D002250:Carbon Radioisotopes; D006020:Glycopeptides; D008565:Membrane Proteins; D009844:Oligosaccharides; D014316:Tritium; D005643:Fucose