title: The plasma protein binding of HIDA.
abstract: By using Sephadex gel column chromatography to separate substances into their various components according to molecular weight, we have investigated the effect of incubating several "brands" of HIDA in plasma, in vitro. The results show that such incubation has no effect on either dimethyl HIDA, or diethyl HIDA, but that in the case of para-butyl HIDA, incubation in plasma increases the Rf value to that of HSA (human serum albumin). This indicates that para-butyl HIDA becomes bound to plasma proteins, in contrast to both dimethyl HIDA and diethyl HIDA.
mesh_terms: D001798:Blood Proteins / Q000378:metabolism*; D006801:Humans; D007098:Imino Acids / Q000378:metabolism*; D066298:In Vitro Techniques; D007558:Italy; D015609:Organotechnetium Compounds; D011485:Protein Binding; D011933:Reagent Kits, Diagnostic; D013557:Switzerland; D013667:Technetium / Q000378:metabolism*; D019784:Technetium Tc 99m Diethyl-iminodiacetic Acid; D019780:Technetium Tc 99m Lidofenin
chemical_list: D001798:Blood Proteins; D007098:Imino Acids; D015609:Organotechnetium Compounds; D011933:Reagent Kits, Diagnostic; D019784:Technetium Tc 99m Diethyl-iminodiacetic Acid; C024340:technetium Tc 99m (para-butyl)iminodiacetic acid; D019780:Technetium Tc 99m Lidofenin; D013667:Technetium