title: Comparison of polysaccharide synthesis between preimplantation stage mouse embryos and F9 embryonal carcinoma cells.
abstract: Polysaccharide synthesis was compared between preimplantation stage mouse embryos and F9 embryonal carcinoma cells. When cells were labelled with [3H]fucose, elution profiles of the pronase digest from a Sephacryl S-200 column were similar for the two cell types and both had characteristic large polysaccharides of the same size (Mr 20-500K), although embryos contained less large polysaccharide than F9 cells, and embryonic polysaccharides were less acidic, suggesting poor sialosylation. A significant proportion of [3H]mannose was incorporated into complex-type glycopeptides in F9 cells, whereas in the case of embryonic cells most glycopeptides remained as high mannose-type. The most striking difference was observed when cells were labelled with galactose. In the case of embryonic cells, 90% of the radioactivity was incorporated into glycogen, whereas only 0.1% of the incorporated galactose occurred in this molecule in F9 cells. These observations indicate that carbohydrate metabolism of morula-stage embryos is very different from that of F9 cells.
mesh_terms: D000818:Animals; D002460:Cell Line; D002970:Cleavage Stage, Ovum / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism; D006003:Glycogen / Q000032:analysis; D008358:Mannose / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D009028:Morula / Q000378:metabolism*; D011134:Polysaccharides / Q000032:analysis / Q000096:biosynthesis*; D013724:Teratoma / Q000378:metabolism*
chemical_list: D011134:Polysaccharides; D006003:Glycogen; D008358:Mannose; D005690:Galactose