title: The role of the Wolffian ducts in the formation of the sinus vagina: an organ culture study.
abstract: In mammals formation of a sinus vagina is inhibited in the male by endogenous testosterone from the embryonic testes. To answer the question which morphogenetic events during formation of the vagina are influenced by testosterone, we explanted genital tracts of mouse embryos in the indifferent stage of development in organ culture. Half of the explants were treated with testosterone and therefore developed in male direction. The other half was kept without testosterone and developed constitutively in female direction. Since the anti Müller factor was not present, in both types of cultures the Müllerian ducts were preserved. During female development the Müllerian ducts fused with the dorsolaterally apposed caudal segments of the Wolffian ducts. Thus the caudal segments of the Wolffian ducts were incorporated in the vaginal plate, while cranially the Wolffian ducts degenerated as expected. During male development fusion between Müllerian and Wolffian ducts did not occur. Under the influence of testosterone the respective caudal segments of the Wolffian ducts were surrounded by dense mesenchyme and further male differentiation took place. We conclude that the 'sinus protrusions' or 'sinovaginal bulbs' observed during development of the vagina, are in fact the caudal segments of the Wolffian ducts. They serve as a link between Müllerian ducts and urogenital sinus. Formation of a sinus vagina is prevented by testosterone simply by induction of male development in this area.
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009095:Mullerian Ducts / Q000033:anatomy & histology; D009924:Organ Culture Techniques; D012733:Sex Differentiation; D013739:Testosterone / Q000502:physiology; D013997:Time Factors; D014621:Vagina / Q000196:embryology*; D014928:Wolffian Ducts / Q000033:anatomy & histology*
chemical_list: D013739:Testosterone