In vivo sister chromatid exchange analysis of a mouse plasmacytoma cell line NP-38.
In vivo sister chromatid exchange (SCE) frequencies have been compared between the mouse plasmacytoma NP-38 and normal bone marrow cells of the host BALB/c mouse. NP-38 cells, transplanted subcutaneously showed a two-fold increase in SCEs (4.35-5.76/cell) compared with the bone marrow cells of the host (1.65-2.14/cell). Such an increase in SCE rates was also observed in NP-38 cells metastasized in spleen, bone marrow, liver, or mesentery, upon inoculation of NP-38 cells by intravenous injection. Even in such tumor-bearing mice, the SCE rates of the bone marrow cells were equivalent to the SCE level found in uninfected mice. These results indicate that the high SCE incidence in NP-38 cells is an inherent characteristic of this tumor cell line.
D000818:Animals; D001853:Bone Marrow / Q000648:ultrastructure*; D001855:Bone Marrow Diseases / Q000473:pathology; D002460:Cell Line; D003434:Crossing Over, Genetic; D008113:Liver Neoplasms / Q000556:secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000235:genetics; D010954:Plasmacytoma / Q000235:genetics* / Q000556:secondary; D012854:Sister Chromatid Exchange; D013160:Splenic Neoplasms / Q000556:secondary