title: Morphometric evaluation of the hypothalamic-ovarian axis of the ketonuric, diabetic Chinese hamster: relationship to the reproductive cycle.
abstract: The relationship between diabetes and the morphological alterations which occur in hypothalamic and ovarian tissue was examined in the long-term, ketonuric-diabetic Chinese hamster. Matched diabetic and non-diabetic control hamsters were inspected daily for changes in the reproductive cycle by vaginal lavage. On dioestrus, animals were perfused, the hypothalamus and ovaries collected, prepared for microscopy and morphometrically analyzed. The nuclei in the medial basal hypothalamus of diabetic hamsters exhibited a decreased area (p less than 0.01) and neuronal population (p less than 0.05-0.01) compared with controls. The ovaries of the diabetic animals had a reduced follicular population (p less than or equal to 0.05) and an increased atresia rate (p less than or equal to 0.05) compared with controls. In addition, all diabetic hamsters were acyclic. In diabetic animals, the corpora luteal cells contained a reduced lipid content (p less than or equal to 0.001) which was possibly functionally related to a significant decline in serum progesterone levels (p less than or equal to 0.01). Based on these results it is suggested that the hypothalamic-ovarian axis is both morphologically and functionally impaired in the diabetic hamster.
mesh_terms: D000818:Animals; D002452:Cell Count; D003338:Corpus Luteum / Q000473:pathology; D006224:Cricetinae; D003412:Cricetulus; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000473:pathology* / Q000652:urine; D004031:Diestrus; D004958:Estradiol / Q000097:blood; D005260:Female; D007031:Hypothalamus / Q000473:pathology*; D007657:Ketone Bodies / Q000652:urine; D006080:Ovarian Follicle / Q000473:pathology; D010053:Ovary / Q000473:pathology*; D011247:Pregnancy; D011374:Progesterone / Q000097:blood
chemical_list: D007657:Ketone Bodies; D011374:Progesterone; D004958:Estradiol