Acylation of 1-palmitoyl-lysophosphatidylglycerol in a alveolar type II cells from rat lung.
1. Alveolar type II cells from adult rat lung have the enzymic capability to acylate 1-palmitoyl-lysophosphatidyl-glycerol, using palmitoyl-CoA to form dipalmitoylphosphatidylglycerol. 2. On a protein basis the acylation of 1-palmitoyl-lysophosphatidylglycerol is at least 2-fold more active in sonicated type II cells than in whole lung homogenates. 3. Both type II cells and whole lung homogenates show higher activity towards palmitoyl-CoA than towards oleoyl-CoA for acylation of 1-palmitoyl-lysophosphatidylglycerol. 4. Both in type II cells and in whole lung homogenates the rates of acylation of 1-palmitoyl-lysophosphatidylglycerol and 1-palmitoyl-lysophosphatidylcholine with palmitate are of the same order of magnitude, while the rate of acylation of 1-palmitoyl-lysophosphatidylethanolamine is much lower.
D000214:Acyl Coenzyme A / Q000378:metabolism; D000215:Acylation; D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D008244:Lysophosphatidylcholines / Q000378:metabolism*; D008246:Lysophospholipids; D008297:Male; D009829:Oleic Acids / Q000378:metabolism; D010171:Palmitoyl Coenzyme A / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000378:metabolism*; D011663:Pulmonary Surfactants / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains
D000214:Acyl Coenzyme A; D008244:Lysophosphatidylcholines; D008246:Lysophospholipids; D009829:Oleic Acids; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D011663:Pulmonary Surfactants; C008301:lysophosphatidylethanolamine; C026223:lysophosphatidylglycerol; C006065:We 201; C017585:oleoyl-coenzyme A; D010171:Palmitoyl Coenzyme A; D000217:Acyltransferases