title: Lipids in the developing enamel of the rat incisor. Parallel histochemical and biochemical investigations.
abstract: Morphologic examination of the developing enamel of rat incisors showed the presence of cell processes and remnants. Histochemical investigation of rapid-frozen freeze-substituted samples, using p-phenylenediamine or a phosphotungstic acid chromic acid mixture, revealed osmiophilic components which were extractable in chloroform-methanol solution and were located inside the tubule-like structures of the extracellular matrix. The presecretory cell-rich and developing enamel zones underwent quantitative and qualitative lipid analysis. Comparison of the biochemical data as well as of the morphological observations, suggests a cellular origin for enamel lipids randomly adsorbed by extracellular matrix components during enamel processing. The nature of the material which appeared as an osmiophilic intra-tubular filling is still unresolved.
mesh_terms: D000818:Animals; D003743:Dental Enamel / Q000032:analysis* / Q000254:growth & development / Q000648:ultrastructure; D005227:Fatty Acids / Q000032:analysis; D007180:Incisor / Q000032:analysis* / Q000254:growth & development / Q000648:ultrastructure; D008055:Lipids / Q000032:analysis*; D009993:Osmium Tetroxide; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005227:Fatty Acids; D008055:Lipids; D010743:Phospholipids; D009993:Osmium Tetroxide