Isolation of the secretory form of soluble acetylcholinesterase by using affinity chromatography on edrophonium-Sepharose.
A single molecular form of soluble acetylcholinesterase was isolated from a variety of mammalian tissues by use of a novel affinity matrix. This matrix was synthesised by coupling the reversible cholinesterase inhibitor, edrophonium chloride, to epoxy-activated Sepharose. This simple synthesis produced a matrix which was exceptionally stable and had the novel property of selectively binding only one molecular form of acetylcholinesterase. Soluble proteins from a variety of mammalian tissues, including brain, adrenal glands, cerebrospinal fluid, and blood, were separated by centrifugation. These contained combinations of acetylcholinesterase (EC 3.1.1.7) and cholinesterase (EC 3.1.1.8), varying from a single form of acetylcholinesterase to multiple forms of both acetylcholinesterase and cholinesterase. The edrophonium-Sepharose matrix bound only one form of acetylcholinesterase. This form of acetylcholinesterase corresponded in molecular size and electrophoretic mobility to the unique form found in cerebrospinal fluid, i.e. secretory acetylcholinesterase. Cholinesterase was not bound to the matrix.
D000110:Acetylcholinesterase / Q000302:isolation & purification*; D000313:Adrenal Medulla / Q000201:enzymology; D000818:Animals; D001921:Brain / Q000201:enzymology; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D004491:Edrophonium; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006168:Guinea Pigs; D007527:Isoenzymes / Q000302:isolation & purification*; D051379:Mice; D008970:Molecular Weight; D011247:Pregnancy; D011817:Rabbits
D007527:Isoenzymes; D004491:Edrophonium; D000110:Acetylcholinesterase