title: Novel mannose carrier in the trypanosomatid Crithidia fasciculata behaving as a short alpha-saturated polyprenyl phosphate.
abstract: Crithidia fasciculata cells incubated with [14C]glucose or membranes derived from the same protozoan incubated with GDP-[14C]mannose were found to synthesize a lipid monophosphate mannose. No glucosylated mild acid-labile compound was formed in vivo or in vitro when UDP-[14C]glucose was used instead of GDP-[14C]mannose. The lipid moiety of the mannosyl derivative formed behaved as a polyprenol having 11 isoprene residues as judged by t.l.c. and be gel filtration in sodium deoxycholate-containing buffers. The mannolipid was not broken on treatment with hot phenol, suggesting the existence of an alpha-saturated isoprene unit. This is the first case reported in which a mannosyl phospholipid involved in sugar transfer in a eukaryotic cell behaves as if it was similar to that of bacterial polyprenols, although having its putative alpha-isoprene unit saturated to the same extent as dolichols from higher organisms.
mesh_terms: D000818:Animals; D001692:Biological Transport; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D003421:Crithidia / Q000378:metabolism*; D004287:Dolichol Monophosphate Mannose / Q000096:biosynthesis / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism*; D011105:Polyisoprenyl Phosphate Sugars / Q000378:metabolism*
chemical_list: D011105:Polyisoprenyl Phosphate Sugars; D004287:Dolichol Monophosphate Mannose; D008358:Mannose