Genetic recombination in Actinoplanes brasiliensis by protoplast fusion.
Protoplast formation, fusion, and cell regeneration have been achieved with mutant strains of Actinoplanes brasiliensis. Three-, four-, and five-factor crosses have shown genetic recombination among the markers, and a five-factor cross is analyzed and discussed. Possibilities of using protoplast fusion for gene mapping and strain improvement are suggested.
D000192:Actinomycetales / Q000235:genetics* / Q000502:physiology; D003433:Crosses, Genetic; D005798:Genes, Bacterial; D008561:Membrane Fusion; D009154:Mutation; D011523:Protoplasts / Q000502:physiology*; D011995:Recombination, Genetic