Coating of liposomes with subunits of monoclonal IgM antibody and targeting of the liposomes.
SH-bearing subunits of IgM antibody were coupled through the Fc portion to sonicated liposomes consisting of N-(m-maleimidobenzoyl)dipalmitoylphosphatidylethanolamine, dipalmitoylphosphatidylcholine and cholesterol. Liposomes coated with subunits of monoclonal IgM antibody against a mouse mammary tumor-associated antigen selectively bound to the antigenic target cells. Assays of the liposomes for complement dependent cytotoxicity and spleen cell binding revealed inactivation of the liposome-coupled antibodies in both assays.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology; D005260:Female; D007075:Immunoglobulin M / Q000276:immunology*; D008081:Liposomes / Q000008:administration & dosage / Q000276:immunology*; D008301:Maleimides; D008325:Mammary Neoplasms, Experimental / Q000276:immunology; D051379:Mice; D013438:Sulfhydryl Compounds
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D007075:Immunoglobulin M; D008081:Liposomes; D008301:Maleimides; D013438:Sulfhydryl Compounds