Large scale screening programme for selection of antisera for radioimmunoassay of human parathyroid hormone.
A large-scale, three-phase screening programme has been devised for the rapid selection of antisera which might be of potential use in clinical radioimmunoassays for human parathyroid hormone. A total of 122 sera from 169 guinea pigs and 6 rabbits immunized with bovine parathyroid hormone, and 12 guinea pigs immunized with human parathyroid hormone were assessed relative to reference antisera. Pre-determined criteria for the three phase programme were imposed by the requirement for antisera that could be used at dilutions greater than 10(-5) in order to ensure continuity of supplies for wide-spread distribution and by the limited availability of human parathyroid hormone for testing purposes. Of the sera tested, only 5 were selected as having high titre and sensitivity for low concentrations of human parathyroid hormone. The 5 antisera were further evaluated for amino- and carboxyl-region specificities for the human parathyroid hormone in comparison with antiserum 211/32, widely distributed for use in radioimmunoassay for clinical purposes. The selected antisera appear to be of high affinity with good recognition of the whole or carboxyl-region parts of the human parathyroid molecule.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000915:Antibody Affinity; D000918:Antibody Specificity; D002417:Cattle; D006168:Guinea Pigs; D006801:Humans; D007114:Immunization; D007120:Immunochemistry; D010281:Parathyroid Hormone / Q000032:analysis / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*
D000906:Antibodies; D010281:Parathyroid Hormone