A simple and effective method for hemolysis with a hypoxanthine-xanthine oxidase system and alteration of erythrocyte phospholipid composition during the hemolysis.
A very rapid hemolysis was found to be caused by active oxygen species produced by a hypoxanthine-xanthine oxidase system with very low concentrations of hypoxanthine. The addition of superoxide dismutase or catalase inhibited the hemolysis, indicating that O2- and H2O2 participate in this system. The extent of erythrocyte hemolysis was found to depend on the sex of the human donor. The change in phospholipid composition before and after hemolysis in human erythrocytes from donors of each sex was compared by thin layer chromatography. A significant decrease in phosphatidylethanolamine content and a concomitant increase in altered phospholipid fraction were observed in erythrocytes from male donors, suggesting that these erythrocytes were easily attacked by active oxygen species to produce modified phosphatidylethanolamine.
D000328:Adult; D002374:Catalase / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D007042:Hypoxanthines / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D008563:Membrane Lipids / Q000097:blood*; D010084:Oxidation-Reduction / Q000187:drug effects; D010743:Phospholipids / Q000097:blood*; D012737:Sex Factors; D013482:Superoxide Dismutase / Q000494:pharmacology; D014969:Xanthine Oxidase / Q000494:pharmacology*
D007042:Hypoxanthines; D008563:Membrane Lipids; D010743:Phospholipids; D002374:Catalase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase