Immunoglobulin heavy chain class switch from IgM to IgG in a hybridoma.
A subclone of an IgM-producing hybridoma has been identified which has switched to producing an IgG1 antibody. The parent hybridoma, PC-140, produces an antibody which binds phosphorylcholine and reacts with monoclonal antibodies that recognize myelomas of the T-15 idiotype. The IgG1 antibody binds phosphorylcholine with the same affinity as the parental IgM and also reacts with the anti-T-15 monoclonal antibodies. While the IgM-producing parent hybridoma does not express detectable surface IgM, the IgG1-producing subclone produces both membrane and secreted IgG1.
D000818:Animals; D002462:Cell Membrane / Q000276:immunology; D005786:Gene Expression Regulation; D006825:Hybridomas / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D051379:Mice; D010767:Phosphorylcholine / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000096:biosynthesis
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell; D010767:Phosphorylcholine