IgE-mediated 14C-serotonin release from rat mast cells modulated by morphine and endorphins.
The formaldehyde method was used to examine the interaction of PGE1 with morphine, beta-endorphin and Met-enkephalin on rat mast cells by their effects on IgE-mediated 14C-serotonin release. PGE1 (2x10(-8) -2x10(-5) M) caused a dose-related inhibition of the mediator release 1 min after an antigen challenge, and morphine (3x10(-7) -3x10(-5) M) reversed this PGE1 effect dose-dependently and stereospecifically; naloxone (2x10(-4) M) antagonized this action of morphine. Beta-Endorphin (3x10(-7) -10(-5) M) and Met-enkephalin (3x10(-6) -10(-4) M) mimicked this morphine action dose-dependently and were antagonized by naloxone (2x10(-4) M). These results suggest that morphine and endorphins modulate immunological mediator release from rat mast cells through opioid receptors.
D000527:Alprostadil; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004723:Endorphins / Q000494:pharmacology*; D007073:Immunoglobulin E / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D009020:Morphine / Q000494:pharmacology*; D009270:Naloxone / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*
D004723:Endorphins; D011458:Prostaglandins E; D012701:Serotonin; D009270:Naloxone; D007073:Immunoglobulin E; D009020:Morphine; D000527:Alprostadil