Comparative studies of pig platelet membrane proteins.
The proteins and glycoproteins of pig platelet membranes have been studied using gel electrophoretic techniques. A nomenclature is suggested from the apparent molecular weights estimated by one-dimensional electrophoresis. Isoelectric focusing showed that the majority of the proteins are in the 4.0-7.0 pH range. Subunits have been inferred from oligoproteins by two-dimensional, reduced-nonreduced, electrophoresis techniques. High resolution two dimensional electrophoresis combining isoelectric focusing and sodium dodecyl sulphate allows the observations of 60 polypeptide bands. An identification of some of those bands based on a correlation from reported human blood platelet membrane proteins is presented for comparison.
D000818:Animals; D001792:Blood Platelets / Q000032:analysis*; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008565:Membrane Proteins / Q000097:blood*; D008970:Molecular Weight; D010084:Oxidation-Reduction; D013552:Swine / Q000097:blood*
D001798:Blood Proteins; D008565:Membrane Proteins