N-(5-dimethylaminonaphthalene-1-sulfonyl)-3-aminobenzene boronic acid as an active-site-directed fluorescent probe of lipoprotein lipase.
Resonance energy transfer was used to monitor the interaction of an active-site-directed fluorescent inhibitor, N-(5-dimethylaminonaphthalene-1-sulfonyl)-3-aminobenzene boronic acid, and lipoprotein lipase (EC 3.1.1.34). The binding of this probe to the active site of lipoprotein lipase had an association constant, Ka, of 1.1 X 10(6) M-1, indicating a strong interaction. The binding was displaced competitively by benzene boronic acid. The method described provides a sensitive procedure to probe the active site of lipoprotein lipase.
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D001897:Boronic Acids / Q000378:metabolism*; D002417:Cattle; D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008892:Milk / Q000201:enzymology; D013050:Spectrometry, Fluorescence
D001897:Boronic Acids; D003619:Dansyl Compounds; D005456:Fluorescent Dyes; C032882:N-dansyl-3-aminobenzeneboronic acid; D008071:Lipoprotein Lipase