Serum rapidly mobilizes calcium from an intracellular pool in quiescent fibroblastic cells.
Addition of dialysed fetal bovine serum to quiescent cultures of Swiss 3T3 cells loaded with 45Ca2+ causes a very rapid increase in the rate of 45Ca2+ efflux from an intracellular pool. Exposure to serum for 2 min leads to a fall of 0.59 nmol Ca2+/mg protein in the intracellular Ca2+ content of the cells. Inhibitors of mitochondrial function prevent the stimulation of 45Ca2+ efflux by serum. The stimulation of 45Ca2+ efflux by serum is also observed in quiescent cultures of Rat-1, Swiss 3T6 and BHK cells and in secondary cultures of whole mouse embryo fibroblasts.
D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000097:blood / Q000378:metabolism*; D002132:Calcium Radioisotopes; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D007700:Kinetics; D051379:Mice; D008928:Mitochondria / Q000187:drug effects; D012402:Rotenone / Q000494:pharmacology; D012427:Rutamycin / Q000494:pharmacology
D002132:Calcium Radioisotopes; D003470:Culture Media; D012402:Rotenone; D012427:Rutamycin; D002118:Calcium