Measurement of granulocyte migration and accumulation in inflammation in man.
A method has been developed for studying granulocyte kinetics in inflammation. Autologous granulocytes isolated from whole blood and labelled with 111indium, without separation from plasma enriched media, localized rapidly in intra-abdominal abscesses and inflammatory bowel disease following intravenous reinjection. Utilizing a gamma camera interfaced to a computer for dynamic imaging, uptake of activity in the site of inflammation could be demonstrated from 10 min following injection. A quantitative estimate of the rate of granulocyte uptake in inflammatory foci, (early granulocyte accumulation index EGAI) was made by subtracting the gradient of the time activity profile, recorded over the inflammatory focus from that of the profile recorded over a similar sized control region, and expressing the difference in relation to the zero time intercept of the profile over the inflammatory focus. The EGAI showed a positive correlation with the ESR (sigma = 0.574; P less than 0.05) and, in the single patient studied twice, decreased following steroid therapy. This technique should prove useful in studying granulocyte kinetics in inflammation in man.
D000005:Abdomen; D000038:Abscess / Q000473:pathology; D002465:Cell Movement; D003093:Colitis, Ulcerative / Q000473:pathology; D003424:Crohn Disease / Q000473:pathology; D006098:Granulocytes / Q000502:physiology*; D006801:Humans; D007204:Indium; D007249:Inflammation / Q000473:pathology*; D011868:Radioisotopes
D011868:Radioisotopes; D007204:Indium