Nucleotide sequence of Scenedesmus obliquus cytoplasmic initiator tRNA.
The cytoplasmic initiator tRNA from the green alga Scenedesmus obliquus has been purified and its sequence shown to be p A G C U G A G-U m G m G C G C A G D G G A A G C G psi m G A psi G G G C U C A U t A A--C C C A U A G m G D m C A C A G G A U C G m A A A C C U Gm U C U C A--G C U A C C A-O H. The sequence has been deduced and confirmed using several different P-post labelling techniques. The sequence is similar to those of other eukaryotic cytoplasmic initiator tRNAs and it has the sequence G A U C in place of the usual G T psi C. Although it resembles lower eukaryotic species in having a U preceding the anticodon and a modified G in the T psi C stem, in overall homology it is closer to the higher eukaryotic than to the fungal initiator tRNAs.
D001483:Base Sequence; D000460:Chlorophyta / Q000235:genetics*; D003593:Cytoplasm / Q000378:metabolism; D005057:Eukaryotic Cells / Q000378:metabolism; D005658:Fungi / Q000235:genetics; D008715:Methionine / Q000378:metabolism; D008722:Methods; D012343:RNA, Transfer / Q000235:genetics*; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics
D012346:RNA, Transfer, Amino Acyl; D012343:RNA, Transfer; D008715:Methionine