[Biological activity of tRNA in rat liver during cycloheximide-blocked translation].
The aminoacylation of tRNA was investigated with respect to protein synthesis in the rat liver. No correlation was found between the 85-90% inhibition of protein synthesis 2 h after cycloheximide injection and aminoacylation level of some tRNAs both in vivo and in vitro. A decrease in aminoacylation (28%) was established only for lysine. During the recovery phase of protein synthesis 12 and 24 h after cycloheximide treatment the aminoacylation maximal level of mixture with 14C amino acids, 14C leucine, 14C glutamic acid was unchanged.
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D014176:Protein Biosynthesis / Q000187:drug effects*; D012343:RNA, Transfer / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D051381:Rats
D012346:RNA, Transfer, Amino Acyl; D012343:RNA, Transfer; D003513:Cycloheximide
Biologicheskaia aktivnost' tRNK pecheni krys pri blokirovanii transliatsii tsiklogeksimidom.