Alkaline phosphatase activity of chronic granulocytic leukaemia neutrophils in agar culture.
We measured the concentration of granulocyte-committed progenitor cells (CFU-c) and the alkaline phosphatase activity of neutrophils harvested from colonies cultured from the peripheral blood of 30 patients with high-count chronic granulocytic leukaemia (CGL) in the chronic phase. Neutrophils in colonies cultured from marrow of normal donors or patients with acute myeloid leukaemia in remission served as controls. CFU-c numbers in CGL peripheral blood were on average 3 times higher than in normal marrow (130.1 +/- 126.2 (SD) versus 43.0 +/- 25.2 per 1 X 10(5) cells plated, respectively). The mean NAP scores in cultured CGL neutrophils were substantially higher than control values (89.9 +/- 48.0 versus 55.9 +/- 33.0 units, respectively). There was a tendency for peak values of NA in culture to be reached at earlier points in CGL cultures with high CFU-c numbers (i.e. high proliferative rates) than in those with lower CFU-c numbers (lower proliferative rates). Our data accord with the concept that low NA levels in CGL in vivo result from modulating influences external to the neutrophil.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000469:Alkaline Phosphatase / Q000097:blood*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005260:Female; D006412:Hematopoietic Stem Cells / Q000201:enzymology; D006801:Humans; D007951:Leukemia, Myeloid / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology*
D000469:Alkaline Phosphatase