Selenium-containing tRNAs from Clostridium sticklandii. Cochromatography of seleno-tRNA I with L-VALYL-tRNA.
It has been previously shown that Clostridium sticklandii specifically synthesized three readily separable 75Se-labeled tRNAs, designated seleno-tRNAs I, II and III, and the partially purified seleno-tRNA II cochromatographed with L-prolyl-tRNA on DEAE-Sephadex A-50 (Chen, C.S. and Stadtman, T.C. (1980) Proc. Natl. Acad. Sci. U.S.A. 77, 1403-1407). In the present study a highly purified 75Se-labeled tRNA I was obtained by chromatography on benzoylated DEAE-cellulose, DEAE-Sephadex A-50 and Sepharose 4B. The 75Se-labeled tRNA I cochromatographed with an L-valine-accepting species on DEAE-Sephadex A-50 and Sepharose 4B. Addition of a 285-fold molar excess of unlabeled L-valine to the L-valine acceptor activity assay mixture markedly decreased the amount of L-[14C]valine bound to seleno-tRNA I.
D002852:Chromatography, Ion Exchange; D003013:Clostridium / Q000235:genetics*; D012343:RNA, Transfer / Q000302:isolation & purification*; D012346:RNA, Transfer, Amino Acyl / Q000302:isolation & purification* / Q000378:metabolism; D012643:Selenium / Q000302:isolation & purification*; D014633:Valine / Q000378:metabolism
D012346:RNA, Transfer, Amino Acyl; C366009:seleno-tRNA; D012343:RNA, Transfer; D012643:Selenium; D014633:Valine