[Use of a chromatographic method for determining kallikrein and prekallikrein in dog blood serum].
The authors determined the conditions of chromatography to be used for determination of prekallikrein and kallikrein in the dog blood serum. The enzyme and its precursor do not adsorb on DEAE-Sephadex A-50 in a 0.02 M phosphate buffer, pH 7, containing 0.15 M NaCl, and thus their quantity can be determined in a non-adsorbed protein fraction from the rate of hydrolysis of N-benzoyl-L-arginine ethyl ester before and after exposure to trypsin. In 1 ml of the dog blood serum, kallikrein activity ranged from 0 to 60 mE, while prekallikrein activity from 127 to 187 mE.
D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004285:Dogs; D007610:Kallikreins / Q000032:analysis* / Q000097:blood* / Q000302:isolation & purification; D011288:Prekallikrein / Q000032:analysis* / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet
D011288:Prekallikrein; D007610:Kallikreins
Ispol'zovanie khromatograficheskogo metoda dlia opredaleniia kallikreina i prekallikreina v syvorotke krovi sobaki.