DNA synthesis of enamel organ epithelium in vitro is enhanced by co-cultivation with non-viable mesenchyme cells.
Developing enamel organ epithelium was cultured in the presence and absence of non-viable dental papilla mesenchyme cells. Epithelial cell proliferation was enhanced by 75% when cultivated on the mesenchyme cell substratum. The addition of epidermal growth factor (EGF) to enamel organ epithelium cultured on this substratum resulted in a synergistic enhancement of DNA synthesis.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis*; D004658:Enamel Organ / Q000166:cytology / Q000378:metabolism*; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D009024:Morphogenesis; D009805:Odontogenesis; D014083:Tooth Germ / Q000378:metabolism*; D014316:Tritium
D014316:Tritium; D004247:DNA