A method for determination of human urinary inactive kallikrein (prekallikrein).
Inactive kallikrein (prekallikrein) in human urine was discovered recently. A more accurate method to measure this zymogen and total kallikrein is described. With trypsin pretreatment, it was found that 35.3 +/- 2.7% of total urinary kallikrein activity was prekallikrein. The results are supported by a new direct radioimmunoassay which measures predominantly active kallikrein.
D000328:Adult; D000368:Aged; D004032:Diet; D005260:Female; D006801:Humans; D007610:Kallikreins / Q000652:urine*; D008297:Male; D008722:Methods; D008875:Middle Aged; D011288:Prekallikrein / Q000652:urine*; D011863:Radioimmunoassay; D012964:Sodium / Q000494:pharmacology; D014106:Tosylarginine Methyl Ester; D014357:Trypsin / Q000494:pharmacology
D014106:Tosylarginine Methyl Ester; D011288:Prekallikrein; D012964:Sodium; D007610:Kallikreins; D014357:Trypsin