Changes of post-transcriptional modification of wye base in tumor-specific tRNAPhe.
Nucleotide sequences of normal mouse liver tRNAPhe and tumor-specific tRNAPhes isolated from Ehrlich ascites tumor and neuroblastoma cells were examined by post-labeling techniques. The results showed that their sequences are identical, except for changes in post-transcriptional modifications that are located in the anticodon region. Normal mouse liver tRNAPhe contained Cm32, Gm34 and YOH37. On the other hand, tumor-specific tRNAPhes were found in one of two possible configurations: 1) Cm32, Gm34 and Y*OH37 (under-modified YOH) or 2) C32, G34 and m1G37. The ratio of the two forms of tRNAPhes differed in different tumor cells; Ehrlich ascites tumor tRNAPhe had mainly Y*OH-containing tRNAPhe whereas neuroblastoma tRNAPhe has predominantly m1G-containing tRNAPhe. It was concluded that tumor-specific tRNAPhes are products of different extents of modification, rather than of new tRNA transcription.
D000818:Animals; D000926:Anticodon / Q000235:genetics; D001483:Base Sequence; D002286:Carcinoma, Ehrlich Tumor / Q000235:genetics*; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D051379:Mice; D009374:Neoplasms, Experimental / Q000235:genetics; D009447:Neuroblastoma / Q000235:genetics*; D009690:Nucleic Acid Conformation; D012323:RNA Processing, Post-Transcriptional; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics* / Q000302:isolation & purification
D000926:Anticodon; D012346:RNA, Transfer, Amino Acyl; C012099:wye; D006147:Guanine