Method for detection of enzymatic activities which use aminoacyl-tRNA as a substrate.
A method for detection of enzymatic activities which use aminoacyl-tRNA is described. It is based on the synthesis of aminoacyl-tRNA in the reaction mixture (in situ) without additional purification. The results are the same as when using purified AA-tRNA.
D000217:Acyltransferases / Q000032:analysis*; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D019881:Aminoacyltransferases; D055598:Chemical Phenomena; D002621:Chemistry; D007887:Fabaceae / Q000201:enzymology; D010445:Peptide Elongation Factors / Q000032:analysis; D010652:Phenylalanine-tRNA Ligase / Q000378:metabolism; D010946:Plants, Medicinal; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D012639:Seeds / Q000201:enzymology
D010445:Peptide Elongation Factors; D012346:RNA, Transfer, Amino Acyl; D000217:Acyltransferases; D019881:Aminoacyltransferases; C019675:tRNA-protein transferase, amino acyl; D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase