Bifunctional enzyme activity at the same active site: study of 3 alpha and 20 beta activity by affinity alkylation of 3 alpha, 20 beta-hydroxysteroid dehydrogenase with 17-(bromoacetoxy)steroids.
D015089:20-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000345:Affinity Labels; D000478:Alkylation; D001665:Binding Sites; D003349:Cortisone Reductase / Q000378:metabolism*; D013196:Dihydrotestosterone / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D006908:Hydroxyprogesterones / Q000138:chemical synthesis*; D007700:Kinetics; D008722:Methods; D011485:Protein Binding; D013302:Streptomyces / Q000201:enzymology; D013379:Substrate Specificity
D000345:Affinity Labels; D006908:Hydroxyprogesterones; D013196:Dihydrotestosterone; C023561:17-(bromoacetoxy)progesterone; C023562:dihydrotestosterone 17-bromoacetate; D015089:20-Hydroxysteroid Dehydrogenases; D003349:Cortisone Reductase