The inability of superoxide dismutase to inhibit the depolymerization of hyaluronic acid by ferrous ions and ascorbate.
Superoxide radicals were investigated as to their capability of depolymerizing the hyaluronic acid of the bovine vitreous body. Using viscometry it was found that O2 radicals, generated by the hypoxanthine/xanthine oxidase method or the combination of NADH and phenazine methosulphate, degraded hyaluronic acid. This reaction was suppressed by superoxide dismutase, catalase, and peroxidase. In contrast, the depolymerization of hyaluronic acid by oxidation-reduction systems like ascorbic acid or ferrous ions was abolished by catalase and peroxidase while superoxide dismutase showed no effect.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology; D002417:Cattle; D005296:Ferrous Compounds / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D010544:Peroxidases / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology*; D014822:Vitreous Body / Q000187:drug effects* / Q000378:metabolism
D005296:Ferrous Compounds; D006820:Hyaluronic Acid; D007501:Iron; D010544:Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid