Primary enzyme quantitation using substrates labeled with a second indicator enzyme. I. Elastase determination using peroxidase-labeled elastin.
D000818:Animals; D052160:Benzothiazoles; D002118:Calcium; D004549:Elastin / Q000031:analogs & derivatives*; D006735:Horseradish Peroxidase / Q000032:analysis; D006861:Hydrogen Peroxide; D010179:Pancreas / Q000201:enzymology; D010196:Pancreatic Elastase / Q000032:analysis*; D010544:Peroxidases / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods; D013451:Sulfonic Acids; D013552:Swine
D052160:Benzothiazoles; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D004549:Elastin; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D010544:Peroxidases; D010196:Pancreatic Elastase; D002118:Calcium