Isolation of hybrid cell clones that contain deletion and non-deletion defects of alpha-thalassemia in man.
We have succeeded in isolating hybrid mouse erythroleukemia cell clones from a patient with hemoglobin H disease, which exhibit either deletion or nondeletion mutations of the human alpha-globin genes. Analysis of one of these hybrid clones that had retained a human chromosome 16 from the patient's cells showed that both human alpha-globin had been deleted. Several clones of another hybrid cell had retained a human chromsome 16 from the patient's cells, which contained both human alpha-globin genes on an EcoRI fragment of 23 kilobases (kb). These latter hybrid clones showed the presence of human alpha-globin chains at detectable but low levels. These studies show that there are two different types of human chromosome 16 in this patient and that the nondeletion mutation of human alpha-globin genes leading to hemoglobin H diseases in this patient acts in cis to the two alpha-globin genes remaining in his cells. The close correlation between the pattern of human alpha-globin gene expression in the patient and in the hybrid cells suggests that this method of transfer of human globin genes to rodent cells will be a useful one for study of mutations affecting the expression of differentiated genes that lead to disease in man.
D000818:Animals; D002459:Cell Fusion; D002469:Cell Separation; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D002902:Chromosomes, Human, 16-18; D002999:Clone Cells; D004247:DNA; D005914:Globins / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D006824:Hybridization, Genetic; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics; D051379:Mice; D013789:Thalassemia / Q000235:genetics*
D005914:Globins; D004247:DNA