Evaluation of the 3 alpha-hydroxysteroid dehydrogenase assay for ursodeoxycholic acid, and 7 oxo- and 12 oxo- bile acids.
3 alpha-Hydroxysteroid dehydrogenase (SDH) quantifies ursodeoxycholic acid, 3 alpha,12 alpha-diol-7-one-5 beta-cholanoic acid, 3 alpha-ol-7-one-5 beta-cholanoic acid, and 3 alpha,7 alpha-diol-12-one-5 beta-cholanoic acid in a manner similar to the more commonly measured bile acids, exemplified by taurocholic acid. The type of oxygen function at the 7 or 12 position and its orientation at the 7 position has no effect on the rate of reaction of the enzyme. Discrepancies in the glycine/taurine ratios of patient intestinal aspirates containing ursodeoxycholic acid and oxo bile acids, obtained by SDH and gas-liquid chromatographic methods are not a result of the enzyme assay procedure.
D015096:3-Hydroxysteroid Dehydrogenases; D001646:Bile / Q000032:analysis; D001647:Bile Acids and Salts / Q000032:analysis; D002635:Chenodeoxycholic Acid / Q000031:analogs & derivatives / Q000032:analysis; D002793:Cholic Acids / Q000032:analysis*; D002849:Chromatography, Gas; D003840:Deoxycholic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D005998:Glycine / Q000032:analysis; D006801:Humans; D007422:Intestines / Q000032:analysis; D008095:Lithocholic Acid / Q000031:analogs & derivatives / Q000032:analysis; D013654:Taurine / Q000032:analysis; D014580:Ursodeoxycholic Acid / Q000032:analysis*
D001647:Bile Acids and Salts; D002793:Cholic Acids; D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; C023867:7-ketodeoxycholic acid; D013654:Taurine; C025576:3,7-dihydroxy-12-oxocholanoic acid; C023020:7-ketolithocholic acid; D008095:Lithocholic Acid; D014580:Ursodeoxycholic Acid; D015096:3-Hydroxysteroid Dehydrogenases; D005998:Glycine