Differentiation of human leukemias in response  to 12-0-tetradecanoylphorbol-13-acetate in vitro.
Leukemic cells from patients with acute myeloid leukemia underwent morphological, functional, and histochemical changes within 24-48 hr after treatment with 1.6 x 10-18 M 12-0-tetradecanoylphorbol-13-acetate (TPA). The changes included adhesion to the plastic substrate, a 4-6-fold increase in the number of phagocytic cells, and an increase in the number of alpha-naphthyl-acetate esterase (alpha-NAE) positive cells. In contrast, TPA treatment of cells from patients with acute lymphoblastic leukemia caused some aggregation of cells in suspension, but no changes in adhesion, phagocytosis, or alpha-NAE. Of the four cases of undifferentiated or unclassified leukemias studied, two failed to respond to TPA, one responded with a myeloid (adhesion) pattern, and one with a lymphoid (aggregation) pattern. These data suggest that leukemic myeloblasts retain the ability to express a variety of differentiated functions, and in some cases, it may be possible to use TPA as a tool to test the differentiative potential of undifferentiated human leukemias.
D002448:Cell Adhesion; D002449:Cell Aggregation; D002471:Cell Transformation, Neoplastic; D004950:Esterases / Q000378:metabolism; D006801:Humans; D007938:Leukemia / Q000473:pathology* / Q000503:physiopathology; D007951:Leukemia, Myeloid / Q000201:enzymology; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology; D010587:Phagocytosis; D010704:Phorbols / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D010704:Phorbols; D004950:Esterases; D013755:Tetradecanoylphorbol Acetate