Gas chromatography-chemical ionization mass spectrometry of cocaine and its metabolites in biological fluids.
A gas chromatographic-chemical ionization mass spectrometric (GC-CIMS) method is described for the determination of cocaine, benzoylecgonine, and norcocaine. The procedure uses stable isotopes as internal standards and a mixture of methane-ammonia as chemical ionization reagent gas. Run-to-run and within-run coefficients of variation (%) are less than 10% and the method has a sensitivity of less than 5 ng/mL from 1 mL or 1 gram of sample. The procedure has been applied to a number of cases involving cocaine intoxication and analytical data from these are described.
D001826:Body Fluids / Q000032:analysis; D001923:Brain Chemistry; D002849:Chromatography, Gas / Q000379:methods; D003042:Cocaine / Q000031:analogs & derivatives / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods
C008671:norcocaine; C005618:benzoylecgonine; D003042:Cocaine