Organ chemiluminescence: noninvasive assay for oxidative radical reactions.
In situ and perfused rat livers showed a spontaneous chemiluminescence of 7-12 counts/sec . cm2 (corresponding to 7-12 x 10(3) photons/sec . cm2); chemiluminescence was increased up to 30 times by infusion of exogenous hydroperoxides. The chemiluminescence of the perfused liver was oxygen dependent. Ethyl, t-butyl, and cumene hydroperoxides were almost equally effective in inducing light emission in the perfused liver. Glutathione release and chemiluminescence showed a parallel increase upon hydroperoxide supply to the perfused liver. A partial spectral analysis of the chemiluminescence of the perfused liver showed a predominance of red-light-emitting species, presumably arising from the singlet oxygen dimol-emission peaks. Many side reactions derived from the complex free radical sequence of lipid peroxidation could afford the chemistry leading to light emission, which represents only about 10(-14) of the utilization of peroxide.
D000818:Animals; D005609:Free Radicals; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008163:Luminescent Measurements; D008297:Male; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*; D010545:Peroxides; D051381:Rats; D013057:Spectrum Analysis
D005609:Free Radicals; D010545:Peroxides; D010544:Peroxidases; D005978:Glutathione