The human tumor-bearing limb: an ex vivo model.
The present report describes a viable, isolated, perfused, human, tumor-bearing limb model suitable for the study of human host-tumor metabolism. The model is hemodynamically stable with high flow rates (50 cc/kg/min) and low perfusion pressures (mean, 77.2 mm Hg) which are stable over time. Metabolically, the preparation is viable with initial oxygen and glucose uptake 0.88 cc/kg/min and 0.029 mmole/kg/min, respectively. These parameters and lactate concentrations do not change significantly with time.
D000671:Amputation, Surgical; D001859:Bone Neoplasms / Q000378:metabolism; D005121:Extremities / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007773:Lactates / Q000378:metabolism; D009369:Neoplasms / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism; D010477:Perfusion / Q000295:instrumentation / Q000379:methods*; D012509:Sarcoma / Q000378:metabolism; D012983:Soft Tissue Neoplasms / Q000378:metabolism
D007773:Lactates; D005947:Glucose