[Spectrophotofluorimetric enzymatic determination of total serum bile acids].
A new enzymatic fluorimetric assay for the determination of total serum bile acids is described. The enzyme 3 alpha-idroxysteroid NAD oxireductase by oxydising the free OH in position 3 alpha of bile acid molecule, reduce NAD previously added. Afterwards the hydrogen of the generated NADH is transferred by a diaphorase to resazurin to yield the fluorophore resorufin proportionally to the amount of bile acids in the sample. The assay is simple, accurate, without bilirubin's influence and useful for a routine use because the analysis time is not different from the more common laboratory tests.
D015096:3-Hydroxysteroid Dehydrogenases; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific); D000328:Adult; D001647:Bile Acids and Salts / Q000032:analysis / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D013050:Spectrometry, Fluorescence
D001647:Bile Acids and Salts; D015096:3-Hydroxysteroid Dehydrogenases; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)
Determinazione enzimatica spettrofotofluorimetrica degli acidi biliari totali serici.