A new screening test for C3 nephritis factor based on a stable cell bound convertase on sheep erythrocytes.
C3 nephritis factor (C3nef) activity was measured by the incubation of sheep erythrocytes with a mixture of normal human serum and patient's serum in EGTA followed by lysis of the washed cells with rat C in EDTA. The C convertase activity on the cells was dependent on the dose of patient's serum. Activation of human C3-C9 by the washed cells was shown by lysis of susceptible target cells (chicken erythrocytes). The test is specific for C3nef.
D000818:Animals; D002645:Chickens; D003166:Complement Activating Enzymes / Q000378:metabolism*; D003167:Complement Activation; D003178:Complement C3 Nephritic Factor / Q000032:analysis*; D050577:Complement C3-C5 Convertases / Q000378:metabolism*; D003169:Complement Inactivator Proteins / Q000032:analysis*; D004306:Dose-Response Relationship, Immunologic; D004912:Erythrocytes / Q000201:enzymology*; D005921:Glomerulonephritis / Q000276:immunology; D006461:Hemolysis; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007700:Kinetics; D051381:Rats; D011919:Rats, Inbred Strains; D012756:Sheep
D003178:Complement C3 Nephritic Factor; D003169:Complement Inactivator Proteins; D003166:Complement Activating Enzymes; D050577:Complement C3-C5 Convertases