N,N-dimethylformamide-induced modulation of organ- and tumor-associated markers in cultured human colon carcinoma cells.
Cultured human colon carcinoma cells were induced by the polar solvent N,N-dimethylformamide (DMF) to express a more differentiated phenotype as indicated by three types of antigen markers. Carcinoembryonic antigen expression in all DMF-induced cell lines was enhanced. Exposure of cells to DMF resulted in a reduction in ability to bind antibody to tumor-derived colon mucoprotein antigen (CMA) with a concomitant gain in binding of antibody to normal CMA. DMF-induced differentiation was further indicated by modulation of blood group antigen expression. DMF treatment decreased the amount of expression of the H-gene determinant. When DMF-treated cells were cultured in the absence of DMF for 14 days, the levels of expression of the antigen markers reverted to those characteristic of their untreated counterparts.
D000017:ABO Blood-Group System; D000230:Adenocarcinoma / Q000188:drug therapy; D000941:Antigens; D002272:Carcinoembryonic Antigen; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D003106:Colon / Q000276:immunology; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000276:immunology; D004126:Dimethylformamide / Q000494:pharmacology*; D006801:Humans; D009088:Mucoproteins / Q000276:immunology; D012397:Rosette Formation
D000017:ABO Blood-Group System; D000941:Antigens; D002272:Carcinoembryonic Antigen; D009088:Mucoproteins; D004126:Dimethylformamide