The conversion of human complement component C5 into fragment C5b by the alternative-pathway C5 convertase.
The cleavage of human complement component C5 to fragment C5b by the alternative pathway C5 convertase was studied. The alternative-pathway C5 convertase on zymosan can be represented by the empirical formula zymosan--C3b2BbP. Both properdin-stabilized C3 and C5 convertase activities decay with a half life of 34 min correlating with the loss of the Bb subunit. The C5 convertase functions in a stepwise fashion: first, C5 binds to C3b and this is followed by cleavage of C5 to C5b. The capacity to bind C3b is a stable feature of component C5, as C5b also has this binding capacity. Component C5, unlike component C3, does not form covalent bonds with zymosan after activation, and C5 is not inhibited by amines. Therefore C5, although similar in structure to C3, does not appear to contain the internal thioester group reported for C3 and C4.
D000588:Amines / Q000494:pharmacology; D003166:Complement Activating Enzymes / Q000378:metabolism*; D003167:Complement Activation; D003176:Complement C3 / Q000378:metabolism; D050577:Complement C3-C5 Convertases; D003179:Complement C3b / Q000378:metabolism; D003182:Complement C5 / Q000378:metabolism*; D050776:Complement C5b; D003170:Complement Pathway, Alternative; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D013438:Sulfhydryl Compounds / Q000032:analysis; D015054:Zymosan / Q000378:metabolism
D000588:Amines; D003176:Complement C3; D003182:Complement C5; D013438:Sulfhydryl Compounds; D003179:Complement C3b; D050776:Complement C5b; D015054:Zymosan; D003166:Complement Activating Enzymes; D050577:Complement C3-C5 Convertases