Limited DNase I nicking as a probe of gene conformation.
We have extended the use of pancreatic DNase I as a probe of chromatin structure by exploring the accessibility of an active gene to the introduction of the first single-stranded nick. We show by a target analysis that the beta-globin gene is about 25-fold more sensitive to single-site nicking than is an average sequence in the chicken erythrocyte nucleus or the nontranscribed albumin gene. The sites of initial DNase I nicking are shown to cluster within the transcribed sequence of the beta-globin gene.
D000418:Albumins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002843:Chromatin / Q000648:ultrastructure*; D003851:Deoxyribonucleases / Q000378:metabolism*; D004912:Erythrocytes / Q000502:physiology; D005260:Female; D005796:Genes; D005914:Globins / Q000235:genetics*; D008970:Molecular Weight; D009707:Nucleosomes / Q000648:ultrastructure; D010057:Oviducts / Q000502:physiology; D014158:Transcription, Genetic
D000418:Albumins; D002843:Chromatin; D009707:Nucleosomes; D005914:Globins; D003851:Deoxyribonucleases