An elastase-specific inhibitor from human bronchial mucus. Isolation and characterization.
It was shown previously that human bronchial mucus contains an acid-stable proteinase inhibitor directed against trypsin and chymotrypsin, polymorphonuclear granulocyte elastase and cathepsin G. In addition to this well-characterized inhibitor, designated here as BSI-ATE (identical with the inhibitor HUSI-I from human seminal plasma or antileucoprotease), another acid-stable inhibitor BSI-E is present in the mucus which exerts inhibitory activity towards porcine pancreatic and human granulocytic elastase, but not against trypsin, chymotrypsin, or granulocytic cathepsin G. This elastase-specific inhibitor was isolated by affinity chromatography. Its molecular mass and its amino acid composition are very similar to those of BSI-TE. An immunological cross-reactivity between both inhibitor species was not observed. In the mucus of patients suffering from obstructive airway disease the elastase-specific inhibitor is not present in the free form but can be liberated by acidification.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001980:Bronchi / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000037:antagonists & inhibitors; D006801:Humans; D007962:Leukocytes / Q000201:enzymology; D009092:Mucous Membrane / Q000737:chemistry*; D010179:Pancreas / Q000201:enzymology; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors* / Q000097:blood; D011480:Protease Inhibitors / Q000494:pharmacology; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D013183:Sputum / Q000737:chemistry; D013552:Swine
D000596:Amino Acids; D004800:Enzymes, Immobilized; D011480:Protease Inhibitors; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D010196:Pancreatic Elastase