Elastinolytic activity in the rat aortic smooth muscle cells in culture.
Elastinolytic activity was examined in cultured rat aortic smooth muscle cells, using Congo Red elastin as a substrate. Elastinolytic activity was demonstrated in the soluble fraction of sonicated smooth muscle cells, with an optimal pH around 10.0. The soluble fraction also showed elastase-like esterolytic activity against the synthetic substrate, N-succinyl-trialanyl-paranitroanilide.
D000818:Animals; D001011:Aorta / Q000166:cytology*; D002478:Cells, Cultured; D004549:Elastin; D006863:Hydrogen-Ion Concentration; D008297:Male; D009130:Muscle, Smooth / Q000166:cytology*; D010196:Pancreatic Elastase / Q000378:metabolism*; D051381:Rats; D013010:Sonication; D013997:Time Factors
D004549:Elastin; D010196:Pancreatic Elastase