The prevention of contamination of teeth stored for transplantation.
Tissue culture media and their application to the storage of teeth are described. The problems of contamination in culture and a method of achieving a sterility level of 73 percent are discussed. Contamination was reduced at each stage, using unerupted teeth, chlorhexidine disinfection, saline irrigation, ultrasonics, a laminar-flow cabinet, and four antibiotics in culture. A method of microbiologic screening is outlined.
D001419:Bacteria; D003470:Culture Media; D046508:Culture Techniques; D003782:Dental Pulp / Q000502:physiology; D004203:Disinfection / Q000379:methods*; D006801:Humans; D010513:Periodontal Ligament / Q000502:physiology; D013048:Specimen Handling; D013242:Sterilization / Q000379:methods*; D014021:Tissue Preservation; D014070:Tooth / Q000637:transplantation*
D003470:Culture Media