Cleavage of peptide-4-nitroanilide substrates with varying chain length by human leukocyte elastase.
Two series of substrates with the formulas H-Alan-Val-4-nitroanilide and 3-carboxypropionyl-Alan-Val-4-nitroanilide (n = 1, 2 and 3) were synthesized. Elongation of the peptide chain yields increasingly better substrates for human leukocyte elastase both in terms of Km and kcat. These results indicate an extended substrate binding site for the enzyme.
D006801:Humans; D007700:Kinetics; D007962:Leukocytes / Q000201:enzymology*; D009842:Oligopeptides / Q000138:chemical synthesis*; D010196:Pancreatic Elastase / Q000097:blood*; D013379:Substrate Specificity
D009842:Oligopeptides; D010196:Pancreatic Elastase