[Conformation and Raman spectra of the transfer ribonucleic acid, tRNAAsp].
The structure of yeast tRNAasp in aqueous solution has been studied in sight of Raman spectra recorded between 5 and 82 degrees C. A conformational change is evidenced at 20 degrees C and an endomelting is found around 70 degrees C. This melting temperature, much higher than in tRNA-phe (near 50 degrees C) is interpreted by the presence of a higher number of G-C bases in t RNA asp. At a same temperature, the Raman spectrum of a tRNAasp crystal is quasi-identical than that of an aqueous solution, indicating a high structural similarity except bands corresponding to G, C bases which show a more effective stacking of these bases in the solid.
D009690:Nucleic Acid Conformation; D012343:RNA, Transfer / Q000032:analysis*; D012346:RNA, Transfer, Amino Acyl / Q000032:analysis*; D012996:Solutions; D013059:Spectrum Analysis, Raman; D013696:Temperature
D012346:RNA, Transfer, Amino Acyl; D012996:Solutions; D012343:RNA, Transfer
Conformations et spectre Raman de l'acide ribonucléique de transfert, tRNAAsp.