Characterization of a rat tRNA gene cluster containing the genes for tRNAAsp, tRNAGly, and tRNAGlu, and pseudogenes.
Molecular cloning and sequence analysis of a rat DNA fragment hybridized to purified tRNAAsp revealed that the genes for tRNAAspGAU(C), tRNAGlyGGA(G), and tRNAGluGAG are in a cluster on the rat chromosome. These genes are arranged in this order and are regularly separated by DNA regions of about 450 bp. The cluster is reiterated about 10 times on the haploid DNA and is present exclusively in a 3.3 kb region cleaved by restriction endonuclease EcoRI. Five lambda clones each containing an independent repeating unit were isolated from a rat gene library. The clones revealed that the length of the repeating unit including the 3.3 kb EcoRI fragment was at least 13.5 kb. Nucleotide sequence analysis and in vitro transcription of the 3.3 kb DNA in the isolated lambda clones showed sequence variations among the repeating units and the presence of incomplete genes for tRNAGly and tRNAGlu within the clusters.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005796:Genes; D008099:Liver / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D012343:RNA, Transfer / Q000235:genetics*; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics; D051381:Rats; D014158:Transcription, Genetic
D012346:RNA, Transfer, Amino Acyl; D012343:RNA, Transfer