[Cryoconservation of 8-celled stage rabbit embryos in an automated "open system"].
Rabbit embryos were successfully frozen at the 8-cell stage, employing a rapid and a slow freezing-thawing program. They were then stored at -196 degrees C (liquid nitrogen) for 10 to 60 days. After thawing the embryos were examined for viability in vitro and in vivo. Using the slow program B 82.5% were morphologically intact, compared to only 69.3% after the rapid procedure (program A). 88.8% of the group first mentioned above, and 56.8% of the latter developed to the blastocyst stage after 4 to 5 days in culture. The implantation rate was 18.1%, when embryos were transferred to minus 18 hours asynchronous recipients, after thawing. With 40%, a significantly higher implantation rate could be achieved, when the embryos were transferred to minus 24 hours asynchronous foster mothers. 18 viable young were born 30 to 32 days after transfer.
D000818:Animals; D001755:Blastocyst; D003201:Computers; D003470:Culture Media; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D005615:Freezing; D009926:Organ Preservation / Q000295:instrumentation / Q000379:methods*; D010062:Ovulation Induction; D011817:Rabbits; D014021:Tissue Preservation / Q000379:methods*; D014022:Tissue Survival
D003470:Culture Media
Kryokonservierung von Kaninchenembryonen des Acht-Zell-Stadiums im automatisierten "Offenen System".