Synthesis and analytical use of 3-carboxypropionyl-alanyl-alanyl-valine-4-nitroanilide: a specific substrate for human leukocyte elastase.
A simple synthesis is described for 3-carboxypropionyl-Ala-Ala-Val-4-nitroanilide, a convenient and very specific substrate for human leukocyte elastase (Km = 1.0mM, kcat = 8.7 s-1). The substrate does not undergo appreciable spontaneous hydrolysis. It is not cleaved by trypsin or chymotrypsin and only rather slowly by porcine pancreatic elastase (Km = 9.1mM, kcat = 1.4 s-1).
D006801:Humans; D007700:Kinetics; D007962:Leukocytes / Q000201:enzymology*; D008722:Methods; D009842:Oligopeptides / Q000138:chemical synthesis*; D010196:Pancreatic Elastase / Q000097:blood*; D013379:Substrate Specificity
D009842:Oligopeptides; C027698:3-carboxypropionyl-alanyl-alanyl-valine-4-nitroanilide; D010196:Pancreatic Elastase