Enzyme-linked immunosorbent assay for the detection of antibodies to type-B influenza virus.
The Enzyme-Linked Immunosorbent assay (ELISA) has been applied to the detection of antibodies to type B influenza virus. The specificity, the sensitivity and the reproducibility of the indirect method has been evaluated. The Haemagglutination inhibition assay (HAI) was used as reference system. The results obtained at a single serum dilution show good correlation between ELISA and HAI test (r = 0.70). Reproducibility is satisfactory. Our results seem to show a better sensitivity of the ELISA test as compared to the HAI test. This feature may be useful for certain applications such as the detection of antibody responses after vaccination. The need of accurate standardization in carrying out the test is pointed out.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D006041:Goats / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007223:Infant; D009981:Influenza B virus / Q000276:immunology*; D008722:Methods; D008875:Middle Aged
D000914:Antibodies, Viral