Interaction of alkaloids with plant transfer ribonucleic acids. Effect of sparteine on lupin arginyl-tRNA formation.
The effect of the alkaloid sparteine on arginyl-tRNA formation was studied. It was demonstrated that sparteine sulfate in the concentration range 10-60 mM inhibits the charging reaction when amino acid, ATP and tRNA are used as variable substrates. The mode of action is different for all pattern of inhibition for all varied substrates is generally uncompetitive. A pattern of inhibition for all varied substrates is generally uncompetitive. A non-competitive mechanism for amino acid and tRNA was observed at low sparteine concentration, but in the case of ATP it is also uncompetitive.
D000470:Alkaloids / Q000494:pharmacology; D001126:Arginine-tRNA Ligase / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000037:antagonists & inhibitors; D013034:Sparteine / Q000494:pharmacology*
D000470:Alkaloids; D012346:RNA, Transfer, Amino Acyl; D013034:Sparteine; D012343:RNA, Transfer; D001126:Arginine-tRNA Ligase