Glyoxylate cycle in toad urinary bladder: possible stimulation by aldosterone.
A homogenate of the toad urinary bladder epithelial cell layer has the two enzymatic activities unique to the glyoxylate cycle--isocitrate lyase (threo-Ds-isocitrate glyoxylate-lyase, EC 4.1.3.1) and malate synthase [L-malate glyoxylate-lyase (CA-acetylating), EC 4.1.3.2]--as well as the capacity to carry out CN-insensitive palmitoyl-CoA oxidation. When tissue is incubated in the presence of a fatty acid substrate, tissue glycogen levels increase. Additionally, in the presence of aldosterone, glycogen levels are higher. These data demonstrate the presence of glyoxylate cycle enzymes in tissue of a higher animal and raise the possibility that such tissue can convert lipid to carbohydrate.
D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D001001:Anura / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005943:Gluconeogenesis / Q000187:drug effects; D006003:Glycogen / Q000378:metabolism; D006038:Glyoxylates / Q000378:metabolism*; D007522:Isocitrate Lyase / Q000378:metabolism; D008292:Malate Synthase / Q000378:metabolism; D008830:Microbodies / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010171:Palmitoyl Coenzyme A / Q000378:metabolism; D001743:Urinary Bladder / Q000378:metabolism*
D005227:Fatty Acids; D006038:Glyoxylates; D009243:NAD; D010171:Palmitoyl Coenzyme A; D000450:Aldosterone; D006003:Glycogen; D008292:Malate Synthase; D007522:Isocitrate Lyase