[Immunochemical studies of human cathepsin D].
Rabbit antiserum against human liver cathepsin D was raised. The antiserum did not cross-react with cathepsins D from tissues of other species (bovine liver, bovine spleen, chicken liver). The study of cathepsins D isolated from human pathologically altered tissues showed that cathepsins from kidney malignant tumor and from myeloleucosis-induced spleen tumor were immunologically identical to the enzyme from normal liver. Cathepsins from liposarcoma and uterine myoma were characterized by partial identity with the enzyme from normal liver. Cathepsins D isolated from various human livers exhibited individual quantitative differences in antigenic properties, a fact to be taken into account in development of an immunochemical method for identification of cathepsin D. The low immunogenicity of human cathepsin D for rabbits and inadequate suitability of these animals for raising appropriate antisera was also considered.
D000818:Animals; D002403:Cathepsins / Q000032:analysis* / Q000276:immunology; D004796:Clinical Enzyme Tests / Q000379:methods*; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification; D007120:Immunochemistry; D007680:Kidney Neoplasms / Q000175:diagnosis; D007889:Leiomyoma / Q000175:diagnosis; D007951:Leukemia, Myeloid / Q000175:diagnosis; D008080:Liposarcoma / Q000175:diagnosis; D008099:Liver / Q000201:enzymology; D011817:Rabbits; D012186:Retroperitoneal Neoplasms / Q000175:diagnosis; D013154:Spleen / Q000201:enzymology; D014594:Uterine Neoplasms / Q000175:diagnosis
D007106:Immune Sera; D002403:Cathepsins
Immunokhimicheskie issledovaniia katepsinov D cheloveka.