A new mouse tumor model system (RIF-1) for comparison of end-point studies.
A new tumor model system (RIF-1) was developed that is very suitable for studies in which clonogenic survival is compared with growth delay and control probability following various forms of treatment. The tumor was a radiation-induced sarcoma in the inbred female C3H/Km mouse. It had a low median tumor dose, had a satisfactory plating efficiency direct from in vivo to in vitro, was nonimmunogenic or minimally immunogenic, and metastasized only at a relatively advanced stage of growth. The cell line grew either as a monolayer on plastic dishes, as tumor spheroids in spinner culture, as lung nodules following injection of a single-cell suspension into the tail veins of syngeneic mice, or as a solid tumor. Both diploid and tetraploid clonogenic cells were found in monolayer cultures of the RIF-1 line.
D000818:Animals; D000912:Antibodies, Neoplasm / Q000096:biosynthesis; D002455:Cell Division; D002869:Chromosome Aberrations; D002999:Clone Cells / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D008175:Lung Neoplasms / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D009381:Neoplasms, Radiation-Induced / Q000473:pathology; D012513:Sarcoma, Experimental / Q000209:etiology / Q000473:pathology; D014185:Transplantation, Isogeneic
D000912:Antibodies, Neoplasm