Structure of genes for virus-associated RNAI and RNAII of adenovirus type 2.
A DNA sequence, 552 base pairs in length, encoding the two "virus-associated" (VA) RNAs of adenovirus type 2 is presented. Comparison of the oligonucleotide maps of VA RNAI and VA RNII with the established sequence permits identification of the genes for these RNAs. VA RNAI is 157-160 nucleotides long and VA RNAII 158-163 nucleotides long, depending on the exact length of their heterogeneous 3' end. The genes are separated by a spacer of about 98 nucleotides. The RNAs exhibit scattered regions of primary sequence homology and can adopt secondary structures which resemble each other closely in their configuration and stability. VA RNAII is also capable of assuming a different configuration that is energetically more favorable. The data suggest that the two RNA genes may have arisen by duplication of an ancestral gene and that the folding of the RNA chain may be of importance for the function of VA RNAs. Hypothetical RNA polymerase III recognition sequences and the coding potential of the region are discussed.
D000260:Adenoviruses, Human / Q000235:genetics*; D001483:Base Sequence; D005075:Biological Evolution; D005796:Genes; D005814:Genes, Viral; D006860:Hydrogen Bonding; D009690:Nucleic Acid Conformation; D009876:Operon; D012320:RNA Polymerase III / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics*
D012367:RNA, Viral; D012320:RNA Polymerase III