Enzymatic determination of total 3 alpha-hydroxy bile acids in faeces. Validation in healthy subjects of a rapid method suitable for clinical routine purpose.
A method for determining faecal bile acids, suitable for clinical purposes, is introduced. The analysis uses a 0.2-g stool specimen, a simple extraction procedure, and 3 alpha-steroid dehydrogenase determination. The method, which is rapid, has been validated by gas-liquid chromatography and by recovery of internal standards. Stool examination was done in 16 healthy volunteers on free diet and in 25 patients with non-gastrointestinal diseases who were on a fat- and fibre-fixed diet. No difference was found between the two groups, so the data were pooled, and the normal reference interval (mean +/- S.D.) for faecal bile acid output was calculated to be 0-975 mumol/24h.
D015096:3-Hydroxysteroid Dehydrogenases / Q000032:analysis*; D000328:Adult; D000368:Aged; D001647:Bile Acids and Salts / Q000032:analysis*; D002849:Chromatography, Gas; D004796:Clinical Enzyme Tests; D005243:Feces / Q000032:analysis* / Q000201:enzymology; D006801:Humans; D008875:Middle Aged
D001647:Bile Acids and Salts; D015096:3-Hydroxysteroid Dehydrogenases