Induction of tumor resistance by hybrid cell lines between human acute lymphocytic leukemia cells and mouse myeloma cells.
Three hybrid cell lines were derived from the fusion of BALB/c mouse NS-1 myeloma cells and human acute lymphocytic leukemia cells. Hybrid clones contained almost all of the mouse chromosomes and a few human chromosomes, and induced tumors in BALB/c and BALB/c nu/nu mice when inoculated subcutaneously. However, the tumors of BALB/c mice subsided spontaneously within one month. The mice which survived the inoculation of 5 approximately 10 X 10(6) hybrid cells were able to reject a subsequent challenge with 5 X 10(6) NS-1 cells. Spleen cells or lymph node cells from immune mice were injected into BALB/c mice. These mice lived longer than the mice which received normal spleen cells or normal lymph node cells, when challenged with 5 X 10(6) NS-1 cells. These results suggest that a rearrangement of tumor-specific antigens on human/mouse hybrid cells can induce immunogenicity.
D000818:Animals; D002460:Cell Line; D005260:Female; D006183:H-2 Antigens / Q000032:analysis; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology / Q000502:physiology*; D007621:Karyotyping; D007945:Leukemia, Lymphoid / Q000235:genetics* / Q000276:immunology; D008677:Metaphase; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010954:Plasmacytoma / Q000235:genetics* / Q000276:immunology
D006183:H-2 Antigens; D006680:HLA Antigens