Effect of uncouplers on radiosensitivity and mutagenicity in x-irradiated mammalian cells.
The number of x-irradiated mammalian cells surviving is markedly increased when the cells are incubated with an uncoupler of oxidative phosphorylation prior to or immediately after irradiation. This increase is greater in plateau-phase cells than in exponentially growing cells. The increase in survival is related to the potency of the uncouplers, which do not modify the effective x-ray dose. The influence of uncouplers on survival is related to an increase of repair and semiconservative DNA synthesis. The mutation frequency (8-azaguanine-resistant mutants) is significantly higher in irradiated cells treated with uncouplers than in untreated cells. These results suggest the existence of an error-prone repair process in mammalian cells.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects; D008114:Liver Neoplasms, Experimental; D009154:Mutation / Q000187:drug effects*; D051381:Rats; D014475:Uncoupling Agents / Q000494:pharmacology*; D014965:X-Rays
D014475:Uncoupling Agents