Fluctuation x-ray scattering from biological particles in frozen solution by using synchrotron radiation.
Determination of the structure of biological particles, randomly oriented in solution, from spatial correlation analysis of fluctuations in x-ray scattering has recently been proposed. The feasibility of scattering fluctuation measurements was evaluated by using an x-ray synchrotron radiation camera to obtain the spatial correlation for a solution of tobacco mosaic virus along a line. The experimental system, analysis of data, and requirements for the determination of structures in solution are discussed using this example.
D005615:Freezing; D008968:Molecular Conformation; D010315:Particle Accelerators; D012542:Scattering, Radiation; D012996:Solutions; D014027:Tobacco Mosaic Virus / Q000648:ultrastructure; D014965:X-Rays
D012996:Solutions