title: Emergence of an epidermis-associated cell surface antigen in epithelial cells of the rat urinary bladder in carcinogenesis.
abstract: Inbred ACI/rats were treated with a selective urinary bladder carcinogen N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN). Two cell surface antigens on the epithelial cells of the bladder, normal bladder epithelium (NBE) antigen unique to normal epithelial cells of the bladder and BC-SK antigen specific for bladder cancer and epidermis, were periodically assayed by mixed hemadsorption test with the typing sera against each antigen. Treatment with BBN for 8 or more weeks induced papillomas or cancers that expressed a definite level of BC-SK antigen, whereas a 4-week treatment did not render the bladder cells with a significant level of this antigen. In general, the level of NBE antigen in bladder cells was not altered by the BBN treatment. In vitro culture of bladder cells of BBN-treated rats resulted in an increase in the level of BC-SK antigen as opposed to a decrease in the NBE antigen level. BC-SK antigen was not detected in epidermal cells of rats younger than 3 days, but it emerged in these cells by in vitro culture for longer than 4 days.
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002085:Butylhydroxybutylnitrosamine; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D004817:Epidermis / Q000276:immunology; D004848:Epithelium / Q000276:immunology; D006382:Hemadsorption; D008297:Male; D009374:Neoplasms, Experimental / Q000276:immunology; D011230:Precancerous Conditions / Q000139:chemically induced* / Q000276:immunology / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D001743:Urinary Bladder / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced* / Q000276:immunology / Q000473:pathology
chemical_list: D000954:Antigens, Surface; D002085:Butylhydroxybutylnitrosamine