Discriminative value of isozymes of amino acid naphthylamidase in the diagnosis of myeloid leukemias.
Isozymes of amino acid naphthylamidase (called B and C) with deviating electrophoretic mobilities were found in peripheral blood leukocytes of 25 out 25 untreated acute and chronic myeloid leukemias (AML and CML) and in 2 out of 2 cases of idiopathic myelofibrosis, while a normal pattern was found in 3 control groups and 5 cases of polycythemia vera. In the AML group, a correlation between electrophoretic mobility and the number of blast cells was found, and on remission, the B isozyme mobility was nearly normalized. Thus, deviating electrophoretic mobility of the B isozyme seemed to be valuable for diagnosis of myeloid leukemias, and in the AML group, the change in mobility might indicate the size of the leukemic clone.
D000328:Adult; D000368:Aged; D000626:Aminopeptidases / Q000097:blood*; D004586:Electrophoresis; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D007945:Leukemia, Lymphoid / Q000201:enzymology; D007951:Leukemia, Myeloid / Q000175:diagnosis*; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis*; D007958:Leukocyte Count; D007962:Leukocytes / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D055728:Primary Myelofibrosis / Q000201:enzymology
D007527:Isoenzymes; D000626:Aminopeptidases