Electron microscopic autoradiography of the trachea in fetal Syrian golden hamsters treated transplacentally with N-nitrosodiethylamine.
Tritiated N-nitrosodiethylamine (DENA) was administered to female Syrian golden hamsters on each of the last 4 days (days 12--15) of pregnancy. The intracellular distribution of bound radioactivity was monitored by electron microscopic autoradiography in the epithelium of the fetal tracheas, from which transplacentally induced tumors arise in this system. Most of the bound radioactivity was concentrated in the rough endoplasmic reticulum of undifferentiated stem cells and mucous cells. The level of binding on the various days of pregnancy appeared to reflect the degree of differentiation of the endoplasmic reticulum in these cells. Basal cells, which were identified as the origin of DENA-induced tracheal tumors in adult hamsters by one or our earlier studies, demonstrated only little bound radioactivity in the fetal tracheas. The pathogenesis of DENA-induced tracheal tumors in this transplacental system may hence be different from that in adult animals.
D000818:Animals; D001345:Autoradiography; D002843:Chromatin; D006224:Cricetinae; D004052:Diethylnitrosamine / Q000378:metabolism* / Q000633:toxicity; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005260:Female; D005865:Gestational Age; D008431:Maternal-Fetal Exchange; D008647:Mesocricetus; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D009602:Nitrosamines / Q000378:metabolism*; D011247:Pregnancy; D014132:Trachea / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D014134:Tracheal Neoplasms / Q000139:chemically induced
D002843:Chromatin; D009602:Nitrosamines; D004052:Diethylnitrosamine