The effects of alkyl-lysophospholipids on leukemic cell lines. I. Differential action on two human leukemic cell lines, HL60 and K562.
The action of an alkyl-lysophospholipid (ALP), ET180CH3, on clonogenicity, 3H-TdR uptake, and cell numbers was tested in two human leukemic cell lines, HL60 and K562, and short-term human leukemic bone marrow cultures. ALP eliminated clonogenicity in HL60 but not in K562 cultures; 3H-TdR uptake and cell numbers were depressed at low concentrations of ET180CH3 in HL60, but not K562 cultures. The action of the lysophospholipid analog on human leukemic bone marrow short-term cultures at low concentrations was similar to its action on HL60 cultures; clonogenicity and 3H-TdR uptake were depressed, but cell numbers were not significantly affected. The demonstration of differential action of ALP on two cell lines should significantly simplify the investigation of the mechanism of the reported differential action of ET180CH3 on normal and leukemic cell membranes.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells / Q000187:drug effects; D003114:Colony-Forming Units Assay; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007942:Leukemia, Experimental / Q000378:metabolism*; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism; D008246:Lysophospholipids; D010743:Phospholipids / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D008246:Lysophospholipids; D010743:Phospholipids; D013936:Thymidine