Reconstitution of the basement membrane from the inner and outer dental epithelia of trypsin-isolated mouse molar enamel organs.
Trypsin-isolated embryonic mouse enamel organs were cultured on top of plasma coagulum. Inner and outer dental epithelia in contact with the plasma coagulum synthesized a new basement membrane as revealed by electron microscopy and 3H-glucosamine autoradiography. The outer dental epithelium in contact with air did not redeposit a basement membrane. The plasma coagulum had beneficial effects on basement membrane reconstitution and maintenance by inhibiting the process of keratinization.
D000818:Animals; D001485:Basement Membrane / Q000502:physiology*; D046508:Culture Techniques; D004658:Enamel Organ / Q000378:metabolism / Q000502:physiology*; D004848:Epithelium / Q000502:physiology; D005944:Glucosamine / Q000378:metabolism; D051379:Mice; D008963:Molar; D012038:Regeneration; D014083:Tooth Germ / Q000502:physiology*; D014316:Tritium
D014316:Tritium; D005944:Glucosamine