title: Aspects of methodology employed in the investigation of combined chemical effects on acute oral toxicity.
abstract: The evaluation of combined chemical effects should be based on the theoretical additive LD50 values which are derived from the LD25 values of the single substances rather than the 1/2 LD50 values. If the latter are used, misinterpretation of the character of the combined action (overestimation of antagonism/underestimation of potentiation) are not excluded, particularly in case of low slope functions.
mesh_terms: D000284:Administration, Oral; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D007928:Lethal Dose 50