An analytic cellular evaluation of aspirated smears from transplanted human endometrial adenocarcinoma in nude mice treated with 17 beta-estradiol and progesterone.
The effects of 17 beta-estradiol and progesterone on human endometrial adenocarcinomas grafted into BALB/c nude mice were studied. Cytologic studies were made on aspiration biopsy smears obtained from the grafts with a fine needle. Mitotic figures were frequently observed in the cells obtained from 17 beta-estradiol-treated mice, whereas there were few mitotic figures in cells from progesterone-treated ones. Progesterone produced such morphologic changes as multinucleolation and cytoplasmic vacuolization. Untreated tumors and those treated with 17 beta-estradiol were solid and were interpreted as poorly differentiated adenocarcinomas. Progesterone-treated tumors were slightly soft but were also interpreted as poorly differentiated adenocarcinomas; however, central necrosis was seen frequently, and differentiation occurred at the margin of these tumors. 17 beta-estradiol promoted increased tumor growth, whereas progesterone produced differentiation and inhibited tumor growth.
D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D001707:Biopsy, Needle; D002455:Cell Division; D002466:Cell Nucleolus / Q000473:pathology; D002467:Cell Nucleus / Q000473:pathology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008940:Mitotic Index; D009368:Neoplasm Transplantation; D011374:Progesterone / Q000494:pharmacology*; D014594:Uterine Neoplasms / Q000473:pathology*; D014617:Vacuoles / Q000473:pathology
D011374:Progesterone; D004958:Estradiol