One-step indirect migration inhibiton (LIF) assay.
A simple, 1-step indirect leukocyte migration inhibition (LIF) assay is described. Advantages of this method are as follows: the mitogen does not directly influence the migration of polymorphonuclear leukocytes, relatively few cells are needed, and results are obtained within 24 h. LIF is simultaneously produced by Con A-pretreated lymphocytes in the migration test system. The lymphokine-producing cells are in the test plate well, in the culture medium surrounding the agarose droplets containing migrating cells. The LIF production can be inhibited by the protein synthesis inhibitor puromycin and by prostaglandin (PGE1). In this test system the LIF production is not influenced by endogenous prostaglandins. This method can be used for complex immunopharmacological investigations because effects of agents on the random migration of polymorphonuclear leukocytes and on LIF production of lymphocytes can be tested in the same system.
D002464:Cell Migration Inhibition; D015232:Dinoprostone; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007962:Leukocytes / Q000276:immunology*; D008222:Lymphokines / Q000096:biosynthesis; D008722:Methods; D011458:Prostaglandins E / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology
D008222:Lymphokines; D011458:Prostaglandins E; D011691:Puromycin; D015232:Dinoprostone; D007213:Indomethacin