Ligand binding of safrole to cytochrome P-450.
Safrole, a hepatocarcinogen, is converted by the microsomal mono-oxygenase system to a reactive intermediate which interacts with cytochrome P-450 to form a ligand complex. The formation of this complex is accompanied by loss of mono-oxygenase activity. The present study describes the interaction of the safrole reactive intermediate with microsomes from phenobarbital, 3-methylcholanthrene and safrole pretreated animals.
D000818:Animals; D001667:Binding, Competitive; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004149:Dioxoles / Q000378:metabolism*; D066298:In Vitro Techniques; D008024:Ligands; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D008862:Microsomes, Liver / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D012451:Safrole / Q000378:metabolism*
D004149:Dioxoles; D008024:Ligands; D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System; D012451:Safrole