[Composition and properties of cholesterol oxidase from Actinomyces lavendulae].
The composition of cholesterol oxidase isolated by different methods from the Actinomyces lavendulae mycelium was studied by gel-chromatography. It was shown that depending on the isolation conditions the enzyme can be detected within the complexes with different molecular weights. The values of pH, pI and Km for the isolated components were determined. The thermal inactivation of the cholesterol oxidase components at different temperature intervals was investigated.
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000190:Actinomyces / Q000201:enzymology*; D002789:Cholesterol Oxidase / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight
D015096:3-Hydroxysteroid Dehydrogenases; D002789:Cholesterol Oxidase
Issledovanie komponentnogo sostava i svoĭstv kholesterinoksidazy Actinomyces lavendulae.