Determination and calibration of crystal lattice images of biological apatites.
Lattice imaging provides detailed information on the mineral phase of calcified tissues. The lattice space distances dhk1 depend directly on the accuracy of the magnification. Using synthetic apatite, K2PtCl6, graphitized C and crocidolite as calibration material the lattice parameters of human enamel have been determined. The relative errors in the mineral dhk1 values using crocidolite, K2PtCl6 and C are 0.6, 1.0 and 3.9 % resp. If hydroxyapatite is employed on the same grid as the enamel specimen, the d-values obtained are very close to the X-ray diffraction data; a reproducibility of 0.8% in a and c lattice parameter determination can be obtained on selected crystallites.
D003461:Crystallography; D003743:Dental Enamel / Q000033:anatomy & histology*; D006801:Humans; D006882:Hydroxyapatites; D008854:Microscopy, Electron
D006882:Hydroxyapatites