Radioimmunoassay for aclacinomycin A.
An antibody against aclacinomycin A (ACM) was produced in a rabbit by immunization with ACM-bovine serum albumin conjugate. The radioimmunoassay (RIA) was based on the competition of unlabeled anthracycline with 3H-labeled ACM for binding sites on a specific antibody. Antibody-bound and free antigen were separated by selective adsorption on dextran-coated charcoal. The antibody reacted equally with ACM and its metabolites, MA144 M1, MA144 S1 and aklavin, and could precisely distinguish aklavinone-related aglycones, adriamycin and daunomycin. RIA was sensitive in the range of 1 approximately 10 pmol per assay. The quantities of ACM and its metabolites in human plasma were practically determined without any pretreatment of physiological samples.
D015250:Aclarubicin; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000097:blood*; D002417:Cattle; D006801:Humans; D008297:Male; D009279:Naphthacenes / Q000097:blood; D011817:Rabbits; D011863:Radioimmunoassay
D000903:Antibiotics, Antineoplastic; D009279:Naphthacenes; D015250:Aclarubicin