A simple and economical device for the rapid collection of multiple supernates from cytotoxicity experiments.
The construction and use of a single semiautomatic device for collecting equal aliquots of supernates after radioisotope release assays of cellular cytotoxicity are described. This device simultaneously removes an aliquot of the supernate from each of the 12 wells in one row of a microtiter plate, and transfers these aliquots directly to disposable tests tubes for insertion into a gamma-counter. Results obtained using this device were more reproducible than manual methods for supernate collection.
D002469:Cell Separation; D002860:Chromium Radioisotopes; D003601:Cytotoxicity Tests, Immunologic / Q000295:instrumentation*; D006801:Humans; D007111:Immunity, Cellular; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis* / Q000276:immunology; D008214:Lymphocytes; D013997:Time Factors
D002860:Chromium Radioisotopes