title: Functional integrity of cryopreserved human monocytes.
abstract: Human peripheral blood monocytes collected by semicontinuous flow centrifugation were cryopreserved in varying concentrations of dimethyl sulfoxide (DMSO). Optimum protection from lethal cryoinjury was achieved with 7.5-10% DMSO. Compared to fresh cells, cryopreserved cells retained 80% of their chemotactic responsiveness and fructose-6-phosphate shunt activity after stimulation by zymosan. Fresh and cryopreserved human monocytes had comparable but low levels of cytotoxic activity against P-815 murine mastocytoma cells in vitro.
mesh_terms: D001793:Blood Preservation; D002469:Cell Separation; D002633:Chemotaxis; D003451:Cryoprotective Agents / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004734:Energy Metabolism; D005615:Freezing; D006801:Humans; D009000:Monocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism
chemical_list: D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide