Isolation of a cDNA clone for the murine transplantation antigen H-2Kb.
A library of cloned cDNAs constructed from the poly(A)+RNA of the murine thymoma cell line EL4 (b haplotype) was screened with a probe encoding a short region of the H-2Kb transplantation antigen. One of the clones isolated, pH202, contains a region that can code for a transplantation antigen with an amino acid sequence 98% homologous to that previously published for H-2Kb. Based on this high degree of homology, pH202 appears to encode the H-2Kb antigen from amino acid 66 through the carboxy terminus, including 386 nucleotides of 3'-untranslated sequence. The amino acid sequence deduced from pH202 suggests that the H-2Kb antigen is actually 2 amino acids longer than previously reported (a total of 348 residues). Four other differences in amino acid assignments are seen. Analysis of the DNA sequences of pH202 and other H-2 clones previously described in the literature suggests that alternative routes of splicing at the 3' end of the coding region are involved in the production of different transplantation antigen mRNAs.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D006183:H-2 Antigens / Q000235:genetics*; D051379:Mice; D011061:Poly A / Q000378:metabolism; D011110:Polymorphism, Genetic; D012326:RNA Splicing
D006183:H-2 Antigens; D011061:Poly A; D004247:DNA