Role of organic phosphate in mineralization of bone in vitro.
Calvarial periostea were dissected from 17-day-old embryonic chicks and folded with the osteogenic cells in apposition. The folded explants were cultured for up to six d on serum and plasma clots or in serum-free hormone-supplemented completely-defined medium. Osteoid consistently formed in such cultures in both types of media, and this osteoid mineralized when appropriate levels of beta-glycerophosphate were added to each type of medium. The data presented suggest that the levels of organic phosphates as a limiting factor in the initiation of mineralization of bone in vitro.
D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D002642:Chick Embryo; D003470:Culture Media; D046508:Culture Techniques; D005994:Glycerophosphates / Q000008:administration & dosage / Q000502:physiology*; D008903:Minerals / Q000378:metabolism*; D010012:Osteogenesis; D010521:Periosteum / Q000378:metabolism
D003470:Culture Media; D005994:Glycerophosphates; D008903:Minerals; C031463:beta-glycerophosphoric acid