Cell cycle-specific mutagenesis at the hypoxanthine phosphoribosyltransferase locus in adult rat liver epithelial cells.
The cell cycle specificity of chemical mutagenesis was studied by use of two cell synchronization techniques, one a nontoxic technique involving serum deprivation and the other a double thymidine block, to obtain rat liver epithelial cells in different phases of the cell cycle to be exposed to chemical mutagens. For both methyl methanesulfonate and N-methyl-N'-nitro-N-nitrosoguanidine, there was a cell cycle specificity of chemical mutagenesis, with the most sensitive phase being the period of DNA synthesis.
D002453:Cell Cycle; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects; D004848:Epithelium / Q000201:enzymology; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D008099:Liver / Q000201:enzymology; D008741:Methyl Methanesulfonate; D008769:Methylnitronitrosoguanidine; D008938:Mitosis; D009153:Mutagens
D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D008741:Methyl Methanesulfonate; D007041:Hypoxanthine Phosphoribosyltransferase