title: The expression of HLA on two cultured choriocarcinoma cell lines.
abstract: Since gestational choriocarcinoma is believed to be derived from trophoblast, paternal antigens are expected to be present on carcinoma cells. Two choriocarcinoma lines (GCC-SV and Lu-75) transplantable in nude mice were obtained and cultures were started in order to study the expression of HLA on these tumor cells. To date, two human chorionic gonadotropin-producing cell lines, designated GCC-SV(c) and Lu-75(c), have been maintained for over two years with more than 15 passages and have been employed for serological studies. First, GCC-SV(c) (patient A2, AW30, B8, B10; husband A3, AW26, BW16, BW35) was tested by protein A assay with HLA-A typing sera and anti-beta 2 microglobulin; the phenotype was A2+, A3-, AW26-, beta 2+, which corresponded to HLA-A type of the patient. In contrast, HLA-A was not demonstrated on Lu-75(c) cells (patient A2, BW35, BW39; husband A9, AW26, BW22) by the direct test, but anti-beta 2 microglobulin serum gave a positive reaction. A mouse monoclonal antibody against HLA was also employed, but it did not react against Lu-75(c) cells. Further serological analysis was carried out by means of absorption tests, and it was confirmed that GCC-SV(c) was A2+, A3-, AW26- and Lu-75(c) was A2-, A9-, AW26-. The results so far obtained do not demonstrate the expression of paternal HLA-A antigens on choriocarcinoma cells.
mesh_terms: D000328:Adult; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002822:Choriocarcinoma / Q000276:immunology* / Q000473:pathology; D005260:Female; D006680:HLA Antigens / Q000235:genetics*; D015234:HLA-A Antigens; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology / Q000473:pathology; D010641:Phenotype; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000276:immunology* / Q000473:pathology
chemical_list: D006680:HLA Antigens; D015234:HLA-A Antigens