title: Effects of local anesthetics on aerobic and anaerobic metabolism of the dental pulp.
abstract: Pulps from rat incisor have been dissected out and the respiratory activity in the tissues has been measured in a Gilson respirometer (modified Warburg technique). Local anesthetics lidocain (xylocain) and prilocain (citanest) in concentrations 2.5%, 5%, and 10% have been added to the medium. Effects on the membrane permeability i.e. on the potassium-sodium pump has been studied using incubation at 37 degrees C in 24NaCl. The influx of 24Na has been registered in a scintillation counter. The anaerobic respiratory activity has been studied determining lactate dehydrogenase activity with a spectrophotometric technique. The results show that the concentrations of local anesthetics in the medium of 5% and 10% caused a significantly lower respiratory activity of the cells in the dental pulp. This was not the case in the concentration 2.5%. The isotope studies show a decreased uptake in all concentrations indicating a depression of the potassium-sodium pump activity. The lactate dehydrogenase activity was significantly lower in all concentrations of local anesthetics.
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D003782:Dental Pulp / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008012:Lidocaine / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects; D011318:Prilocaine / Q000494:pharmacology*; D051381:Rats; D012964:Sodium / Q000378:metabolism
chemical_list: D011318:Prilocaine; D008012:Lidocaine; D012964:Sodium; D007770:L-Lactate Dehydrogenase