Introduction of a human X-6 translocation chromosome into a mouse teratocarcinoma: investigation of control of HLA-A, B, C expression.
We have developed an approach to human developmental biology which exploits somatic cell genetics. With this system we have examined the production of the HLA-A,B,C antigens, A human-mouse somatic cell hybrid was constructed which contained a human X-7 chromosome translocation carrying the HLA region; this hybrid was used as a donor of the X-6 translocation in the technique of microcell transfer. The X-6 chromosome recipient was the mouse embryonal carcinoma cell line PCC4. The microcell hybrid MCP-6 retained the embryonal carcinoma phenotype as judged by shape and absence of H-2 expression. Nonetheless, the expression of the HLA-A,B,C genes was not extinguished. HLA-A,B,C antigen production of the cell surface, however, was not detected because this hybrid apparently could not make beta 2-microglobulin.
D000818:Animals; D002051:Burkitt Lymphoma; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002906:Chromosomes, Human, 6-12 and X / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation; D006680:HLA Antigens / Q000235:genetics*; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology*; D051379:Mice; D013724:Teratoma; D014178:Translocation, Genetic; D014960:X Chromosome
D006680:HLA Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens