Characterization of osteoblast-like cells from fetal rat calvaria.
An improved method is described for the isolation of osteoblast-like cells from fetal rat calvaria. The cells are mononuclear, 8.3 mu in diameter, alkaline phosphate positive, and possess rough endoplasmic reticulum, mitochondria, Golgi, and cytoplasmic processes. Isolated bone cells synthesize collagen and cholesterol, grow to confluence in primary culture, and respond to hormonal stimulation.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured / Q000166:cytology*; D002784:Cholesterol / Q000096:biosynthesis; D003094:Collagen / Q000096:biosynthesis; D003470:Culture Media; D009924:Organ Culture Techniques; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D012886:Skull / Q000166:cytology / Q000196:embryology*
D003470:Culture Media; D003094:Collagen; D002784:Cholesterol