Interaction of albumin and vincristine with a human lymphoblastic leukemia cell line in vitro.
To investigate the potential influence of albumin on the antitumor effect of vincristine (VCR), cells from a human lymphoblastic leukemia cell line (CEM) were exposed in vitro to albumin and a clinically attainable VCR concentration (10(-7)M). Increasing concentrations of albumin between 1 and 4 g/100 ml resulted in a corresponding reduction of VCR-induced growth suppression. Studies employing tritiated vincristine ([3H]-VCR) revealed a progressive inhibition of uptake of the radio-label into CEM cells with increasing concentrations of albumin. These in vitro data show a previously unrecognized protective effect of increasing concentrations of albumin on the growth of human lymphoblastic leukemia cells exposed to VCR as a consequence of inhibition of drug uptake. Further in vivo pharmacologic studies will be necessary to determine their clinical significance.
D001692:Biological Transport / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002648:Child; D006801:Humans; D007700:Kinetics; D007945:Leukemia, Lymphoid / Q000503:physiopathology*; D012709:Serum Albumin / Q000494:pharmacology*; D014750:Vincristine / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use
D012709:Serum Albumin; D014750:Vincristine