title: Phagocytosis of carbohydrate-modified phospholipid vesicles by macrophage.
abstract: Modification of the surface of distearoyl phosphatidylcholine vesicles with synthetic glycolipids dramatically affects the rate of uptake of these vesicles by mouse peritoneal macrophage. The high rate of uptake of 6-aminomannose-modified vesicles is effectively inhibited by cytochalasin B and chloroquine but not by colchicine, indicating that the mechanisms of vesicle uptake is phagocytosis. Other modified vesicles appear to have some effect on the rate of uptake of 6-aminomannose-modified vesicles suggesting that the various vesicle types compete for the same initial binding sites. Analysis of 6-aminomannose-modified vesicles by gamma-ray perturbed angular correlation spectroscopy shows that the rotational correlation time of the encapsulated 111In3+ does not change when the vesicles associate with macrophage. This result is consistent with transmission electron microscopy, which indicates that the aminomannose-modified vesicles remain intact after phagocytosis as aggregates of fused and intact vesicles surrounded by a single bilayer membrane structure.
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002738:Chloroquine / Q000494:pharmacology; D002784:Cholesterol; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D006017:Glycolipids; D008081:Liposomes; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D010743:Phospholipids
chemical_list: D006017:Glycolipids; D008081:Liposomes; D010743:Phospholipids; D003571:Cytochalasin B; D002738:Chloroquine; D002784:Cholesterol; D003078:Colchicine