Typing of murine cell-surface antigens by cellular radioimmunoassay.
A cellular radioimmunoassay utilizing 125I-labelled Protein A was used for detecting antigen-antibody complexes on glutaraldehyde fixed cells attached to microtiter plates. This method is rapid, sensitive and specific for revealing H-2 private and public specificities as well as Ia and Lyt antigens. As plates may be kept for months, several reactivities can be tested in one step on a large panel rendering a regular supply of animals unnecessary.
D000818:Animals; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000032:analysis*; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D011863:Radioimmunoassay / Q000379:methods*; D013205:Staphylococcal Protein A / Q000276:immunology
D000954:Antigens, Surface; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; D013205:Staphylococcal Protein A