Enzymatic sulfation of cholesterol by rat gastric mucosa.
A sulfotransferase which catalyzes transfer of the sulfate group from 3'-phosphoadenosine-5'phosphosulfate to cholesterol has been demonstrated in the rat gastric mucosa. The product of the reaction was characterized as cholesterol sulfate by two-dimensional thin-layer chromatographic behavior, and gas-liquid chromatography of cholesterol after acid solvolysis. The bulk of enzyme activity was found in the cytosol fraction. Sulfation of cholesterol did not require added Mg+2, Mn+2, or Ca+2, and was unaffected by ethylenediaminetetraacetate. Triton X-100 moderately enhanced the enzyme activity. A broad pH optimum from pH 6.0-9.0 was exhibited with a maximum at pH 7.0-7.5. The apparent Km for PAPS was 0.8 x 10(-6)M. The possible function of cholesterol sulfate in gastric mucosa is discussed.
D000818:Animals; D002784:Cholesterol / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D003600:Cytosol / Q000201:enzymology; D005753:Gastric Mucosa / Q000201:enzymology*; D066298:In Vitro Techniques; D008297:Male; D017830:Octoxynol; D010724:Phosphoadenosine Phosphosulfate / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D013379:Substrate Specificity; D013431:Sulfates / Q000378:metabolism*; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000378:metabolism*
D013431:Sulfates; D011092:Polyethylene Glycols; D010724:Phosphoadenosine Phosphosulfate; D017830:Octoxynol; D002784:Cholesterol; C022321:PAPS sulfotransferase; D013466:Sulfurtransferases; D015238:Sulfotransferases