title: G cells and gastrin release from human antral mucosa in vitro.
abstract: Using an in vitro perfusion technique, the release of immunoreactive gastrin (IRG) from human antral mucosa has been investigated, and comparisons made between the release of IRG and the G cell numbers within the tissue. Serosal stimulation with acetyle choline (5.504 x 10(-12)M conc.) resulted in a significantly greater release of IRG from the tissue when compared with release from unstimulated tisse from the same patient (4666 +/- 1656 fM/ml. stimulated; 274 +/- 130 fM/ml. unstimulated (T = 2.64; P < 0.02). A wide variation in G cell numbers was found between tissues and within tissue, and there was no significant correlation between the gastrin secreted and the G cell population. Stimulation with acetyl choline resulted in a 68.8% reduction in G cell numbers compared with a 56.7% reduction in unstimulated tissue, but these were not significantly different (T = 1.68; P > 0.1). No significant correlation was found between the reduction in G cell numbers detected by immunohistology and the IRG secreted by the tissues.
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D002452:Cell Count; D004381:Duodenal Ulcer / Q000473:pathology; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism*; D005755:Gastrins / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D010477:Perfusion; D011706:Pyloric Antrum / Q000166:cytology / Q000378:metabolism*; D011863:Radioimmunoassay
chemical_list: D005755:Gastrins; D000109:Acetylcholine