Quantitative organ culture: an approach to prediction of tumour response.
Quantitative organ culture provides a simple in vitro method for assessing tumour responsiveness. The value of using 125IUdR incorporation as a reproducible index of DNA synthesis in organ culture has been investigated using human benign prostatic hyperplasia. From a study of the variables affecting the reproducibility, sensitivity and specificity of the method a simple technique was adopted. This method was applied to the study of the hormone-sensitivity of breast tumours, some of which showed a dose-response to 17 beta-estradiol. The criteria which must be applied to make such a test of clinical value are discussed.
D001943:Breast Neoplasms / Q000378:metabolism; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006801:Humans; D007065:Idoxuridine / Q000378:metabolism; D008297:Male; D009369:Neoplasms / Q000188:drug therapy*; D009924:Organ Culture Techniques
D004273:DNA, Neoplasm; D004958:Estradiol; D007065:Idoxuridine