Enkephalin biosynthetic pathway: a 5300-dalton adrenal polypeptide that terminates at its COOH end with the sequence [Met]enkephalin-Arg-Gly-Leu-COOH.
An enkephalin-containing polypeptide of 5300 daltons has been isolated from extracts of bovine adrenal medulla and purified to homogeneity, and its sequence has been determined by a combination of automated Edman degradation and enzymatic time-course hydrolysis. The polypeptide contains a single [Met]enkephalin sequence followed by Arg-Gly-Leu-COOH, which forms the COOH terminus of the molecule. The enkephalin sequence is preceded by a Lys-Arg linkage, typical of prohormone cleavage sites. Posttranslational processing of the polypeptide at this site would lead to release of the octapeptide [Met]enkephalin-Arg6-Gly7-Leu8.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002837:Chromaffin Granules / Q000032:analysis*; D002838:Chromaffin System / Q000032:analysis*; D004723:Endorphins / Q000096:biosynthesis*; D004744:Enkephalin, Methionine; D004745:Enkephalins / Q000096:biosynthesis*; D010455:Peptides / Q000032:analysis; D011498:Protein Precursors / Q000032:analysis / Q000378:metabolism
D000596:Amino Acids; D004723:Endorphins; D004745:Enkephalins; D010455:Peptides; D011498:Protein Precursors; D004744:Enkephalin, Methionine