Immunological identification of the human erythrocyte glucose transporter.
A rabbit antibody against the human erythrocyte glucose transporter was purified by affinity chromatography and used to determine the distribution of transporter on polyacrylamide gels after electrophoresis in sodium dodecyl sulfate. Fresh erythrocyte ghosts showed transporter only at the broad 55,000 Mr band, as did the isolated transporter. HeLa cell plasma membranes showed a similar band of crossreacting material at Mr 55,000. The amount of crossreacting material in human erythrocyte ghosts and in plasma membranes from human HeLa cells and mouse L-1210 cells was determined in an enzyme-linked immunosorbent assay which gave results consistent with the extent of glucose-reversible binding of cytochalasin B.
D000906:Antibodies; D002352:Carrier Proteins / Q000276:immunology* / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000276:immunology / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D009004:Monosaccharide Transport Proteins; D009005:Monosaccharides / Q000276:immunology* / Q000378:metabolism
D000906:Antibodies; D002352:Carrier Proteins; D009004:Monosaccharide Transport Proteins; D009005:Monosaccharides