X-ray diffraction of strained muscle fibers in rigor.
The effect of strain on the equatorial x-ray diffraction pattern of glycerinated rabbit psoas fibers was studied in the rigor (ATP free) state. Strains between 30 and 100 A per half sarcomere, measured directly by laser diffraction, did not change the intensity ratio, (10)/ . (11). Because the intensity ratio depends on the distribution of mass within the myofilament lattice, the negative result indicates that strain does not change the angle of attachment of the subfragment 1 (S1) moiety of the myosin molecule to the actin filament. The effect of strain on the ordering of the actin filaments also was considered and judged to be negligible.
D000205:Actomyosin; D000818:Animals; D003599:Cytoskeleton / Q000648:ultrastructure*; D009119:Muscle Contraction; D009132:Muscles / Q000648:ultrastructure*; D011817:Rabbits; D012298:Rigor Mortis; D014961:X-Ray Diffraction
D000205:Actomyosin