Interaction between prostaglandins of the E-type with a urinary component from halothane anesthetized rats.
Prostaglandins of the E-type (PGE's) were found to react or combine with a urinary metabolite of Halothane yielding products which were left unrecovered during the purification procedure preceding specific radioimmunoassay of PGE2. The products were retained on sephadex LH-20 columns, and showed on thin layer silica gel plates (TLC) Rf values lower than those of the parent PGE-compounds. The product formation is supposed to involve the beta-hydroxyketone system of PGE, since PG's of the F and A type were unaffected. The product formation could be avoided by inducing anaesthesia with Hexobarbitone and maintaining the anaesthesia with Halothane-nitrous oxide or it could be reversed by adding barbiturates to urine samples obtained from animals anaesthetized with Halothane-nitrous oxide alone. The barbiturates effectively competed with PGE for the metabolite leaving PGE to behave normally on sephadex LH-20 and TLC, thus enabling us to evaluate correctly the PGE2 content by RIA.
D000777:Anesthetics; D000818:Animals; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D015237:Dinoprost; D015232:Dinoprostone; D006221:Halothane / Q000652:urine*; D006591:Hexobarbital; D008297:Male; D009609:Nitrous Oxide; D011454:Prostaglandins A / Q000652:urine; D011458:Prostaglandins E / Q000652:urine*; D011460:Prostaglandins F / Q000652:urine; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains
D000777:Anesthetics; D011454:Prostaglandins A; D011458:Prostaglandins E; D011460:Prostaglandins F; D006591:Hexobarbital; D015237:Dinoprost; D009609:Nitrous Oxide; C100008:prostaglandin A2; D015232:Dinoprostone; D006221:Halothane