HPLC, MS, and pharmacokinetics of melphalan, bisantrene and 13-cis retinoic acid.
High performance liquid chromatographic procedures are described for melphalan, bisantrene, and 13-cis retinoic acid, three important anticancer drugs in various stages of clinical development. The procedures require a rapid and simple sample clean-up followed by a 10-to 20-min chromatographic separation on a reversed-phase C18 column. Precisions are all less than 8% with recoveries greater than 80%. Mass spectrometry confirmation of each drug from patient sample separations is presented to provide unambiguous identification for valid pharmacokinetic parameter determination.
D000873:Anthracenes / Q000097:blood; D000970:Antineoplastic Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D015474:Isotretinoin; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods; D008558:Melphalan / Q000097:blood*; D014212:Tretinoin / Q000097:blood*
D000873:Anthracenes; D000970:Antineoplastic Agents; C031404:bisantrene; D014212:Tretinoin; D015474:Isotretinoin; D008558:Melphalan