A neural cell adhesion molecule from human brain.
We have used a crossreactive monoclonal antibody against a mouse neural cell adhesion molecule (N-CAM) to characterize and purify an antigen from embryonic and neonatal human brain. This antigen was identified as human N-CAM according to several criteria, including molecular weight, sialic acid content, amino acid composition, analysis of peptide fragments, reactivity with a variety of anti-CAM antibodies, and activity of the antigen in assays of cell and membrane vesicle aggregation. The findings make feasible an exploration of the possible role of N-CAM in various neurological disorders.
D000596:Amino Acids / Q000032:analysis; D000911:Antibodies, Monoclonal; D001923:Brain Chemistry; D002448:Cell Adhesion; D002449:Cell Aggregation; D006801:Humans; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000302:isolation & purification*; D009474:Neurons / Q000032:analysis* / Q000166:cytology
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D009419:Nerve Tissue Proteins