The distal nephron of rat kidney: a target site for glucagon.
Glucagon-sensitive adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] activity was measured in nine different portions of the rat nephron. Each sample contained a single piece of tubule isolated by microdissection from collagenase-treated kidney tissue. As compared to basal activity, 1 microM porcine glucagon stimulated adenylate cyclase 60-fold in the medullary portion and 40-fold in the cortical portion of the thick ascending limb, 23-fold in the early distal convoluted tubule, 11-rold in the cortical collecting tubule, and 8-fold in the medullary collecting tubule. No stimulation was observed in proximaly tubules and thin segments of the loop of Henle. Half-maximal stimulations were obtained with about 10 nM glucagon in the responsive nephron portions.
D000262:Adenylyl Cyclases / Q000032:analysis / Q000378:metabolism; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005934:Glucagon / Q000494:pharmacology* / Q000502:physiology; D007684:Kidney Tubules / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D007685:Kidney Tubules, Collecting / Q000187:drug effects; D007686:Kidney Tubules, Distal / Q000187:drug effects; D051381:Rats
D005934:Glucagon; D000262:Adenylyl Cyclases