Towards standardization of the reconstituted mixed function oxidase systems with cytochrome P-450.
Reconstituted rabbit pulmonary mixed function oxidase (MFO) system with cytochrome P-450 is composed of NADPH cytochrome P-450 reductase, cytochrome P-450, phospholipid extract and cholate. Factors essential for the reconstitution of the active system include the component concentrations and ratios, preincubation and the ionic strength of the buffer. The rabbit pulmonary MFO system can be separated, purified and reconstituted with good reproducibility. However there are many factors influencing reconstituted activity mainly associated with the reconstitution and reductase purification procedure.
D000818:Animals; D002793:Cholic Acids / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000592:standards*; D008168:Lung / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000592:standards*; D009249:NADP / Q000378:metabolism; D010088:Oxidoreductases / Q000592:standards*; D010743:Phospholipids / Q000378:metabolism; D011817:Rabbits; D014468:Umbelliferones / Q000378:metabolism
D002793:Cholic Acids; D010743:Phospholipids; D014468:Umbelliferones; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases