Regional mapping of the gene coding for enolase-2 on human chromosome 12.
Enolase-2 (ENO2), previously termed 14-3-2 protein, is an isozyme of enolase that is enriched in neuronal tissue. The gene coding for ENO2 was previously assigned to human chromosome 12. The present study presents data for a regional mapping of gene ENO2 using cell hybrids containing various deletions of human chromosome 12. These deletions were produced by treatment with chromosome-breaking agents. Cytogenetic analysis has allowed assignment of ENO2 to the short arm of chromosome 12, in the region of pter-p1205. This assignment is consistent with the segregation pattern of the 93 hybrid clones analysed. The segregation pattern has also established the linear order of 6 genes on chromosome 12:pter-TPI-GAPD-LDHB-ENO2-centromere-SHMT-PEPB-qter. Estimation of the relative distances between the 6 genes on chromosome 12 has been made by a statistical mapping analysis of the segregation data of the hybrid clones. A set of deletion hybrids containing various combinations of these 6 markers has been established for a rapid regional mapping of genes in one of these regions on chromosome 12.
D000818:Animals; D002478:Cells, Cultured; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002906:Chromosomes, Human, 6-12 and X; D006224:Cricetinae; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D009447:Neuroblastoma; D010751:Phosphopyruvate Hydratase / Q000235:genetics* / Q000378:metabolism
D005819:Genetic Markers; D010751:Phosphopyruvate Hydratase