title: Luteolysis-induced changes in phase composition and fluidity of bovine luteal cell membranes.
abstract: X-ray diffraction, fluorescence polarization of trans-parinaric acid, and fluorescence photobleaching recovery of dioctadecyltrimethyneindolecarbocyanine have been used to characterize the phase composition and liquid phase fluidity of bovine luteal cell membranes and membrane lipids for functional corpora lutea collected at midcycle and for regressing corpora lutea collected after treatment with prostaglandin F2 alpha. These results support previous observations of gel phases in microsomal preparations of regressed luteal cells at physiological temperatures and further suggest that the plasma membrane may be the main source of this gel phase. Analysis of the overall lipid composition of the microsomal preparations from these cells indicates a role for sphingomyelin, in the presence of cholesterol, for the generation of a gel phase at physiological temperatures.
mesh_terms: D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000502:physiology*; D002784:Cholesterol / Q000494:pharmacology; D003338:Corpus Luteum / Q000648:ultrastructure*; D015237:Dinoprost; D004971:Estrus; D005260:Female; D005454:Fluorescence Polarization; D003341:Luteolysis; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000502:physiology; D010743:Phospholipids / Q000502:physiology; D011247:Pregnancy; D011460:Prostaglandins F / Q000494:pharmacology; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D008563:Membrane Lipids; D010743:Phospholipids; D011460:Prostaglandins F; D002784:Cholesterol; D015237:Dinoprost