Arylsulphatase C and estrone sulphatase of sheep hypothalamus, preoptic area, and midbrain: separation by hydrophobic interaction chromatography and evidence for differences in their lipid environment.
Arylsulphatase C and estrone sulphatase activities of sheep hypothalamus-preoptic area-midbrain were examined for their susceptibility to phospholipase action. Russel's viper phospholipase A could completely inactivate estrone sulphatase without affecting arylsulphatase C. The latter was partially inactivated by S. aureus phospholipase C but not by C. welchi phospholipase C. Both arylsulphatase C and estrone sulphatase were inactivated to different extents by sodium deoxycholate, which is known to activate the intrinsic phospholipases of brain. Hydrophobic interaction chromatography on phenyl-Sepharose resulted in the differential elution of arylsulphatase C and estrone sulphatase. The results suggest that one enzyme is not responsible for arylsulphatase C and estrone sulphatase activities.
D000818:Animals; D001192:Arylsulfatases / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D003840:Deoxycholic Acid / Q000494:pharmacology; D007031:Hypothalamus / Q000201:enzymology*; D008055:Lipids / Q000494:pharmacology*; D008636:Mesencephalon / Q000201:enzymology*; D010740:Phospholipases / Q000494:pharmacology; D011301:Preoptic Area / Q000201:enzymology; D012756:Sheep; D043266:Steryl-Sulfatase; D013429:Sulfatases / Q000037:antagonists & inhibitors / Q000302:isolation & purification*
D008055:Lipids; D003840:Deoxycholic Acid; D010740:Phospholipases; D013429:Sulfatases; C020121:estrone sulfatase; D001192:Arylsulfatases; D043266:Steryl-Sulfatase