Action of mitomycin C on testicular steroidogenesis and its probable mode of action in albino rats.
Testicular steroidogenesis was determined by radioimmuno assay of serum testosterone and histochemical demonstration of the key steroidogenic enzyme (delta 5 3 beta hydroxysteroid dehydrogenase) activity in the Leydig cells after treatment with mitomycin C (mc), (500 microgram/kg, Bwt). In vivo experiments showed mc treatment causes a remarkable fall of serum testosterone level along with significant reduction in the weights of testis and sex accessories. In vitro studies resulted in a diminution in the delta 5 3 beta hydroxysteroid dehydrogenase and glucose-6-phosphate dehydrogenase activity in the section of the testis after addition of mc (100, 200 microgram/ml) in the incubation medium. The consideration of the above findings coroborated to the suggestion that mc reduced testicular steroidogenesis.
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000818:Animals; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007985:Leydig Cells / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008937:Mitomycins / Q000494:pharmacology*; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D013737:Testis / Q000187:drug effects / Q000201:enzymology*; D013739:Testosterone / Q000097:blood*
D008937:Mitomycins; D013739:Testosterone; D015096:3-Hydroxysteroid Dehydrogenases; D005954:Glucosephosphate Dehydrogenase