Preparation of a monoclonal antibody to rat liver glucose-6-phosphate dehydrogenase and the study of its immunoreactivity with native and inactivated enzyme.
A monoclonal antibody of the IgG class was prepared against rat liver glucose-6-phosphate dehydrogenase (G6PD; D-glucose-6-phosphate:NADP+ 1-oxidoreductase, EC 1.1.1.49) by the hybridoma technique. This antibody does not affect the catalytic activity of the enzyme and shows crossreactivity with the palmitoyl CoA-inactivated G6PD. By solid phase radioimmunoassay, the presence of crossreacting materials in comparable amounts was determined in liver homogenate supernatants from rats that had been starved and refed a high-sucrose diet (containing a high level of G6PD activity) and from rats that had been starved and refed a high-fat diet (containing a low level of G6PD activity). These findings indicate that G6PD is present in an inactive form in rats fed a high-fat diet. The monoclonal antibody will facilitate isolation and characterization of the inactive variant G6PD.
D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D003429:Cross Reactions; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors / Q000276:immunology*; D008099:Liver / Q000201:enzymology; D011234:Precipitins; D011487:Protein Conformation; D051381:Rats; D013329:Structure-Activity Relationship
D000911:Antibodies, Monoclonal; D011234:Precipitins; D005954:Glucosephosphate Dehydrogenase