Aspects of methodology employed in the investigation of combined chemical effects on acute oral toxicity.
The evaluation of combined chemical effects should be based on the theoretical additive LD50 values which are derived from the LD25 values of the single substances rather than the 1/2 LD50 values. If the latter are used, misinterpretation of the character of the combined action (overestimation of antagonism/underestimation of potentiation) are not excluded, particularly in case of low slope functions.
D000284:Administration, Oral; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D007928:Lethal Dose 50