title: Evidence for a 3-O-sulfated D-glucosamine residue in the antithrombin-binding sequence of heparin.
abstract: An octasaccharide with high affinity for antithrombin was isolated after partial deaminative cleavage of heparin with nitrous acid. After conversion of the 2,5-anhydro-D-mannose end group to anhydro[1-3H]mannitol, labeled pentasaccharide was released from the octasaccharide by periodate-alkali treatment. Incubation of the pentasaccharide with a recently discovered 3,O-sulfatase from human urine resulted in desulfation, suggesting the occurrence of a 3-sulfate group on the terminal glucosamine residue. The same glucosamine residue was recovered as a 2,5-anhydro[1-3H]mannitol derivative by a procedure involving deamination of the octasaccharide with nitrous acid, reduction of the products with sodium boro[3H]hydride, isolation of 3H-labeled tetrasaccharide by gel chromatography, and release of the labeled end-group by periodate-alkali treatment. Paper electrophoresis indicated disulfated anhydro[3H]mannitol, presumably sulfated at C3 and C6, as a major component, along with smaller amounts of monosulfated (presumably 3-sulfated) anhydro[3H]mannitol. Similar treatment of an analogous tetrasaccharide derived from heparin with low affinity for antithrombin failed to produce any disulfated anhydromannitol. These results suggest that 3-sulfated glucosamine is a unique component of high-affinity heparin, located at a specific position in the antithrombin-binding sequence of the molecule.
mesh_terms: D000818:Animals; D000991:Antithrombins / Q000378:metabolism*; D001665:Binding Sites; D005944:Glucosamine / Q000378:metabolism; D006493:Heparin / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D013431:Sulfates; D013552:Swine
chemical_list: D000991:Antithrombins; D009844:Oligosaccharides; D013431:Sulfates; D006493:Heparin; D005944:Glucosamine