Characterization of a complex Philadelphia translocation (1p-;9q+;22q-) by gene mapping.
Human-Chinese hamster somatic cell hybrids were obtained using circulating leucocytes from a chronic myeloid leukaemia (CML) patients carrying a complex Philadelphia ((Ph1) translocation (1p-;9q+;22q-). Hybrid clones which showed segregation of the translocation chromosomes were studied. The chromosome 22 markers ACO2, ARSA, and NAGA segregated with the 1p- derivative; and the chromosome 1 markers UMPK, PGD, and ENO1 segregated with the 9q+ derivative. Hence, molecular evidence has been obtained for the translocation of the distal part of 22q to chromosome 1 and for the translocation of the distal part of 1p to chromosome 9. No conclusions could be drawn either about translocation of chromosome 9 material or about a possible difference in breakpoint in chromosome 22 when compared with six cases of 9;22 translocations similarly studied and previously reported. In addition, a more precise mapping of PGM1 was obtained, the gene being proximal to UMPK and the breakpoint in 1p32.
D000818:Animals; D002874:Chromosome Mapping; D002900:Chromosomes, Human, 1-3; D002904:Chromosomes, Human, 21-22 and Y; D002906:Chromosomes, Human, 6-12 and X; D006224:Cricetinae; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D007951:Leukemia, Myeloid / Q000235:genetics; D014178:Translocation, Genetic
D005819:Genetic Markers