title: Absence of morphological, chromosomal and antigenic changes in the K-562 cell line growing as localized or disseminated tumours in nude mice.
abstract: Transplantation of K-562 cells into adult and newborn nude mice led to the development of localized s.c. and disseminated myelosarcomas, respectively. This age-associated, changing pattern of in vivo proliferation of K-562 cells derived from a single aliquot was consistently repeated throughout sequential passages. The only variable in this experimental system was the age of the recipient mice. Not only did the mice have an identical genetic background, but also the transplanted K-562 cells were derived from a single culture passage. As shown by cytological and histological examinations, the characteristic morphology and percentage composition of the subpopulations of the K-562 cell line were preserved in successive in vitro and in vivo passages. The K-562 cells had no prevailing phenotypic traits which could be associated with the growth either in the s.c. tissue or in the viscera. Furthermore, the cells maintained the human karyotype, including their typical chromosomal abnormalities and antigenic determinants, as demonstrated by the binding of a specific antibody, throughout all passages. Our results demonstrate that heterotransplanted K-562 cells may change their behaviour in vivo without undergoing modifications associated with different types of growth. These findings would indicate that the ability of neoplastic cells to proliferate in various environments (metastases) is not the consequence of predetermined cellular characteristics but is functionally conditioned.
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm / Q000032:analysis; D002460:Cell Line; D007074:Immunoglobulin G / Q000276:immunology; D007621:Karyotyping; D007942:Leukemia, Experimental / Q000473:pathology; D007951:Leukemia, Myeloid / Q000276:immunology / Q000473:pathology*; D008175:Lung Neoplasms / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude
chemical_list: D000951:Antigens, Neoplasm; D007074:Immunoglobulin G