PHA-induced blast colonies and kinetic parameters in acute myeloid leukemia.
The in vitro leukemic colony-forming ability of 30 acute myeloid leukemias tested after PHA stimulation was analyzed by comparing the CFU-GM growth pattern, the myeloblast [3H]thymidine index and the response to treatment. PHA-induced leukemic colonies were obtained in 15 out of 30 cases. No correlation was found between the FAB classification, the CFU-GM growth pattern and the PHA-induced colony growth. A significant correlation was observed between PHA-induced colony growth and [3H]thymidine labeling index (p less than 0.001). A correction was also noted between the presence of leukemic growth and the failure of the induction treatment (p less than 0.02). The proliferation of the clonogenic blastic subpopulation selected by this technique seems to be linked to a poor prognosis.
D002455:Cell Division; D002469:Cell Separation; D002999:Clone Cells; D006098:Granulocytes / Q000473:pathology; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D007700:Kinetics; D007951:Leukemia, Myeloid / Q000473:pathology; D015470:Leukemia, Myeloid, Acute / Q000145:classification / Q000473:pathology*; D009000:Monocytes / Q000473:pathology; D010835:Phytohemagglutinins / Q000494:pharmacology*; D011379:Prognosis; D013936:Thymidine
D010835:Phytohemagglutinins; D013936:Thymidine