A nondenaturing zwitterionic detergent for membrane biochemistry: design and synthesis.
The synthesis and evaluation of a new detergent that is a zwitterionic derivative of cholic acid is presented. This detergent combines the useful properties of both the sulfobetaine-type detergents and the bile salt anions. The new detergent proved to be effective at solubilizing membrane proteins in a nondenatured state.
D000818:Animals; D002793:Cholic Acids / Q000138:chemical synthesis* / Q000494:pharmacology; D003902:Detergents / Q000138:chemical synthesis / Q000494:pharmacology; D008565:Membrane Proteins / Q000302:isolation & purification*; D051379:Mice; D008862:Microsomes, Liver / Q000032:analysis; D011489:Protein Denaturation / Q000187:drug effects; D013057:Spectrum Analysis; D013501:Surface-Active Agents / Q000138:chemical synthesis
D002793:Cholic Acids; D003902:Detergents; D008565:Membrane Proteins; D013501:Surface-Active Agents; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate