Monoclonal antimacrophage antibodies: human pulmonary macrophages express HLA-DR (Ia-like) antigens in culture.
Study of human macrophages and their role in disease has been hampered by lack of well-characterized tissue macrophages. We describe a simple procedure to obtain viable adherent cells in good yield from resected lung, characterize the cells as greater than 95% macrophage by standard criteria, and document expression of marker properties previously described in rodent macrophages. Human pulmonary macrophages were used to produce monoclonal antibodies and to study expression of HLA-A,B,C, HLA-DR, and related antigens. A new monoclonal mouse antibody, 18, showed restricted binding to macrophages from human lung or blood and to B lymphoid cells, but did not bind to closely related cells. With antibody 18 and other monoclonal antibodies of known specificity, DA2 and Genox 3.53, we showed that a remarkably high proportion of human pulmonary macrophages express HLA-DR framework and DC1 polymorphic determinants in culture. Although the macrophages differed in source and in their state of activation, single cell analysis revealed no heterogeneity in antigen expression.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002469:Cell Separation; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D008168:Lung / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000276:immunology; D011110:Polymorphism, Genetic
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II