Defective excision repair in a mutant of Micrococcus radiodurans hypermutable by some monofunctional alkylating agents.
The lethal and mutagenic effects of methyl methanesulphonate (MMS), ethyl methanesulphonate (EMS), and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) can be dissociated in a mitomycin C (MTC)-sensitive mutant, strain 302, of Micrococcus radiodurans. As regards lethality 302 is extremely sensitive, compared with the wild type, to MTC and decarbamoyl MTC (DCMTC), slightly sensitive to EMS, MNNG, nitrous acid, 7-bromomethylbenz[alpha]anthracene (BrMBA), and N-acetoxy-N-2-acetylaminofluorene (AAAF), and resistant to MMS, hydroxylamine, and ICR 191G. As regards mutability it is, compared to the wild type, very sensitive to MMS, EMS, and MNNG, and slightly sensitive to hydroxylamine and nitrous acid but not to any other agent examined. Alkaline sucrose gradient studies indicate the 302 does not incise DNA containing BrMBA adducts, although it does incise DNA damaged by AAAF but probably not to the same extent as wild type. We put forward the hypothesis that the hypermutability of 302 is due to the non-removal of bases or nucleotides, modified in exocyclic positions, which have altered base-pairing capabilities, while lethality results from the non-removal of bases or nucleotides, also modified in exocyclic positions, which no longer form hydrogen-bonded base pairs.
D004260:DNA Repair; D004352:Drug Resistance, Microbial; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005804:Genes, Lethal; D006898:Hydroxylamines / Q000494:pharmacology; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D008837:Micrococcus / Q000235:genetics*; D008937:Mitomycins / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation / Q000187:drug effects*; D009608:Nitrous Acid / Q000494:pharmacology
D006898:Hydroxylamines; D008937:Mitomycins; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D005020:Ethyl Methanesulfonate; D008741:Methyl Methanesulfonate; D009608:Nitrous Acid