Macrophage production of prostaglandins: effects of fetal calf serum and diazepam. Use of an improved method for extracting 6-keto-PGF1 alpha.
Mouse peritoneal macrophages were incubated with varying amounts of heat-inactivated fetal calf serum (FCS) and treated with diazepam at the concentrations of 0.5 and 5.0 mug/ml. These macrophage preparations demonstrate that the capacity for synthesis and release of prostaglandins (PGs) E2, F2 alpha, 6-keto-PGF1 alpha and thromboxane TX(B2) is: (a) sensitive to the concentration of FCS in the culture medium, and (b) altered in the presence of diazepam. A radioimmunoassay for 6-keto-PGF1 alpha is also reported. The efficiency of extraction of PGE2 and 6-keto-PGF1 alpha from buffer or plasma samples was greatly improved when a petroleum ether/ether (1/1) solvent system was employed.
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis / Q000302:isolation & purification*; D000818:Animals; D000918:Antibody Specificity; D001769:Blood; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D003975:Diazepam / Q000494:pharmacology*; D005260:Female; D005333:Fetus; D066298:In Vitro Techniques; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D011247:Pregnancy; D011453:Prostaglandins / Q000096:biosynthesis*; D011817:Rabbits / Q000276:immunology; D013929:Thromboxane B2 / Q000097:blood
D003470:Culture Media; D011453:Prostaglandins; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D003975:Diazepam