title: A comparative study of some growth characteristics and cell-surface properties of neoplastic cells.
abstract: Tumour cells from a polyoma-induced ascitic tumour were fractionated on the basis of the electrical charge on the cell surface by free-flow electrophoresis. Several characteristics of tumour cells have been investigated: (1) differences in the proliferation and antigenicity within the tumour at any point in time; (2) variation in proliferative potential with the ageing of the tumour. In early ascitic tumours, electrophoretically fractionated cells exhibit very similar proliferative characteristics. However, most DNA synthesis was found in slow-moving cells. The behaviour of older tumours was different. Proliferative potential and DNA synthesis were weaker and restricted to slow-moving cells, suggesting that fast-moving cells in older tumours were resting cells. An enrichment in immunoglobulin-bearing cells was also found in slow-moving cell fractions, supporting the hypothesis of variability in expression of tumour-specific antigens. The role of cell-surface properties and cell kinetics is discussed in relation to electrical surface charge, which might be involved in cell dissemination and metastasis. Thus, free-flow electrophoresis represents a satisfactory approach to isolate tumour cell subpopulations with characteristics such as high proliferative potential or increased expression of tumour antigens.
mesh_terms: D000818:Animals; D002455:Cell Division; D002462:Cell Membrane / Q000473:pathology; D002469:Cell Separation; D002470:Cell Survival; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004586:Electrophoresis; D007136:Immunoglobulins / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D012516:Osteosarcoma / Q000276:immunology / Q000473:pathology*; D012513:Sarcoma, Experimental / Q000276:immunology / Q000473:pathology
chemical_list: D004273:DNA, Neoplasm; D007136:Immunoglobulins