Effects of diazo-oxo-norleucine on cell kinetics and odontoblast differentiation in cultured embryonic mouse molars.
Diazo-oxo-norleucine (DON), an analogue of glutamine, prevented odontoblast differentiation in cultured tooth germs. Diazo-oxo-norleucine added after the onset of odontoblast differentiation, did not affect the secretion of predentine or the functional differentiation of ameloblasts. DON decreased explant volume and modified cell kinetics, decreasing mitotic index, labelling index and number of grains per nucleus; the 5 phase of the cell cycle was lengthened. These modifications of cell kinetics should be considered when interpreting the effects of DON on odontoblast differentiation.
D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003980:Diazooxonorleucine / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D008940:Mitotic Index; D008963:Molar / Q000166:cytology / Q000196:embryology; D009804:Odontoblasts / Q000187:drug effects*
D001391:Azo Compounds; D003980:Diazooxonorleucine