Comparative study on glycosaminoglycan-sulfotransferases in rat costal cartilage and chick embryo cartilage.
1. The comparative features of glycosaminoglycan-sulfotransferase system in rat costal cartilage and chick-embryo cartilage were examined. Some different properties of glycosaminoglycan-sulfotransferases in the cartilage of both the animals have been observed. 2. This study shows that rat costal cartilage contains more 4-sulfotransferase than 6-sulfotransferase and that that reverse situation pertains to chick-embryo cartilage, although both the rat and chick-embryo cartilage contain two sulfotransferase species. 3. Our preliminary experiments by use of rat sulfotransferases and chick endogenous acceptor or of chick sulfotransferases and rat sulfotransferases and chick endogenous acceptor or of chick sulfotransferases and rat endogenous acceptor suggest that sulfotransferases in a given tissue play a central role in determining which sites of the N-acetylgalactosamine moiety of chondroitin sulfates occurring in the tissue are sulfated.
D000818:Animals; D002356:Cartilage / Q000201:enzymology* / Q000648:ultrastructure; D002642:Chick Embryo; D002712:Chlorides; D002807:Chondroitin / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000378:metabolism; D015636:Magnesium Chloride; D008345:Manganese / Q000378:metabolism; D017895:Manganese Compounds; D010724:Phosphoadenosine Phosphosulfate / Q000378:metabolism; D051381:Rats; D013045:Species Specificity; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000378:metabolism*
D002712:Chlorides; D017895:Manganese Compounds; D015636:Magnesium Chloride; D008345:Manganese; D010724:Phosphoadenosine Phosphosulfate; D002807:Chondroitin; C022321:PAPS sulfotransferase; D013466:Sulfurtransferases; D015238:Sulfotransferases; C019893:chondroitin 4-sulfotransferase; D008274:Magnesium; C025340:manganese chloride