Regulated expression of an extrachromosomal human beta-interferon gene in mouse cells.
Beta(fibroblast)-interferon mRNA and protein are induced by the synthetic double-stranded RNA poly(I) X poly(C) in cultured human fibroblasts. To study the mechanism of this induction, we have isolated a human beta-interferon gene and inserted it in a vector plasmid containing DNA of the bovine papilloma virus. After removal of bacterial plasmid sequences, the bovine papilloma virus-beta-interferon recombinant was used to morphologically transform mouse fibroblasts. Analysis of DNA from the transformed cell lines indicated that this recombinant is propagated as a stable multicopy extrachromosomal element. Human beta-interferon mRNA and protein are inducible by poly(I) X poly(C) in all of these cell lines, and the mRNA is indistinguishable from beta-interferon mRNA synthesized by induced human cells.
D000818:Animals; D002478:Cells, Cultured; D005111:Extrachromosomal Inheritance; D005786:Gene Expression Regulation; D005796:Genes; D006801:Humans; D007370:Interferon Type I / Q000235:genetics*; D051379:Mice; D011070:Poly I-C / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D007370:Interferon Type I; D012333:RNA, Messenger; D011070:Poly I-C