Lipopolysaccharide and pristane-induced peritoneal exudate requirements for plasma tumor cell colony formation.
The role for lipopolysaccharide (LPS) and a pristane-induced peritoneal exudate (PIPE) on the in vitro development of murine plasmacytoma was studied. MOPC-315 cell suspensions showed little tendency for colony formation in a soft agar culture medium. Additions of LPS and PIPE were required for maximal colony formation. The LPS effect was dose-dependent down to nanogram quantities. PIPE prepared from inbred strains of mice not susceptible to pristane-induced plasmacytoma (DBA/2) or from normal peritoneal washings was ineffective. PIPE activity was radioresistant and not transferable by cell-free conditioned medium. Three strains of transplantable plasmacytomas showed colony formation stimulation by LPS plus PIPE, but LPS and PIPE were ineffective with lymphosarcoma P1798.
D000818:Animals; D002273:Carcinogens / Q000009:adverse effects; D002460:Cell Line; D003114:Colony-Forming Units Assay; D005260:Female; D008070:Lipopolysaccharides / Q000009:adverse effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D010534:Peritoneal Neoplasms / Q000139:chemically induced*; D010954:Plasmacytoma / Q000139:chemically induced*; D013729:Terpenes / Q000009:adverse effects*
D002273:Carcinogens; D008070:Lipopolysaccharides; D013729:Terpenes; C009042:pristane