Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal.
Downstream placement of a strong transcriptional termination signal has made possible the cloning of bacteriophage T5 promoters known to exhibit high signal strength. The cloning system constructed contains two easily assayable indicator functions whose expression is controlled by the integration of promoters and terminators, respectively. By assessing transcription within the indicator regions, the efficiency of promoters as well as termination signals can be determined in vitro and in vivo.
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005814:Genes, Viral; D009876:Operon; D010957:Plasmids; D013604:T-Phages / Q000235:genetics; D014158:Transcription, Genetic