title: Coated vesicles from human placenta carry ferritin, transferrin, and immunoglobulin G.
abstract: Coated vesicles prepared by using isotonic gradients retain their contents. Ferritin, transferrin, and immunoglobulin G have been detected in human placental coated vesicles. Triton X-100 extraction of crude coated vesicles removes contaminating uncoated membranes, leaving coated particles and their associated proteins apparently intact; this provides an efficient means of preparing large quantities of coated vesicle proteins. The clathrin cages surround "cores" of structural protein containing polypeptides of 100,000 and 50,000 molecular weight.
mesh_terms: D002966:Clathrin; D003594:Cytoplasmic Granules / Q000378:metabolism*; D004705:Endocytosis; D005260:Female; D005293:Ferritins / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D008565:Membrane Proteins / Q000502:physiology*; D010920:Placenta / Q000378:metabolism*; D011092:Polyethylene Glycols; D011247:Pregnancy; D011955:Receptors, Drug / Q000378:metabolism; D014168:Transferrin / Q000378:metabolism*
chemical_list: D002966:Clathrin; D007074:Immunoglobulin G; D008565:Membrane Proteins; D011955:Receptors, Drug; D014168:Transferrin; D011092:Polyethylene Glycols; D005293:Ferritins