title: Phosphoenolpyruvate-dependent glucose phosphotransferase activity in Streptococcus mitis ATCC 903.
abstract: A constitutive phosphoenolpyruvate (PEP)-dependent glucose phosphotransferase system was found in decryptified cells of Strep. mitis. The system displayed saturation kinetics and the apparent Km value for the non-fermentable analogue 2-deoxyglucose (2DG) was 0.4 mM. Sodium fluoride inhibited the activity when 2-phosphoglycerate was used as the energy source instead of PEP. Intact cells accumulated 2DG and also a derivative that behaved chromatographically like 2DG-6-phosphate. PEP-dependent glucose phosphotransferase activity was also demonstrated in freshly collected plaque.
mesh_terms: D000328:Adult; D001692:Biological Transport; D003773:Dental Plaque / Q000201:enzymology / Q000382:microbiology*; D003847:Deoxyglucose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D008297:Male; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D010766:Phosphorylation; D013291:Streptococcus / Q000201:enzymology*
chemical_list: D003847:Deoxyglucose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D005947:Glucose