Application of differential scanning microcalorimetry to the study of cellular processes: heat production and glucose oxidation of murine macrophages.
Differential scanning microcalorimetry provides a noninvasive method for studying heat evolution in living cells. We used this technique to measure the heat evolved by thioglycollate broth-elicited mouse macrophages, and the effects of NaF, KCN, cycloheximide, and cytochalasins B and D on this parameter. The total heat evolved in the interval 10--37 degrees C scanned at 1 degree C min-1 ranged from 300 to 2500 X 10(-12) cal (1 Cal = 4.184 J) per cell, depending on cell density, glucose concentration, and the presence or absence of various drugs.
D000818:Animals; D002152:Calorimetry, Differential Scanning; D003513:Cycloheximide / Q000494:pharmacology; D003572:Cytochalasins / Q000494:pharmacology; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects*; D006358:Hot Temperature; D007700:Kinetics; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D011190:Potassium Cyanide / Q000494:pharmacology; D012969:Sodium Fluoride / Q000494:pharmacology
D003572:Cytochalasins; D012969:Sodium Fluoride; D003513:Cycloheximide; D005947:Glucose; D011190:Potassium Cyanide