title: Cell-mediated immune response to dog pulp tissue altered by Proco-Sol via the root canal.
abstract: Pulp tissue from three experimental dogs was tested for its antigenicity before and after incubation with Proco-Sol sealer (PS). Cell-mediated skin test reactions showed an increased response to pulp that had been incubated in Proco-Sol, washed, and then injected, as compared with pulp or PS alone. In vitro analysis of cell-mediated immune response (lymphocyte proliferation) showed a marked increase when pulp tissue was incubated in PS and washed, as compared to saline-treated pulp. Comparison of the saline-incubated pulp to the PS-altered pulp indicated that the increase in radioactivity was statistically significant at p less than 0.0001. Thus, Proco-Sol sealer altered dog pulp tissue and rendered it antigenically active, and a specific cell-mediated lymphocyte response was produced.
mesh_terms: D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology; D001729:Bismuth / Q000494:pharmacology*; D003782:Dental Pulp / Q000187:drug effects / Q000276:immunology*; D003790:Dental Pulp Necrosis / Q000503:physiopathology; D004285:Dogs; D007111:Immunity, Cellular / Q000187:drug effects*; D007114:Immunization; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D012117:Resins, Synthetic / Q000494:pharmacology*; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012882:Skin Tests; D013997:Time Factors; D015032:Zinc / Q000494:pharmacology*; D015034:Zinc Oxide / Q000494:pharmacology*
chemical_list: D000941:Antigens; D012117:Resins, Synthetic; D012387:Root Canal Filling Materials; C035615:Proco-Sol; D015032:Zinc; D015034:Zinc Oxide; D001729:Bismuth