[Histochemical and ultrastructural study of the rat oral mucosa, after chronic administration of alcohol].
In order to study the action of alcohol on the oral mucosa, Sprague-Dawley female rats, receiving an alcohol rich diet, were sacrifical as well as their controls, 98, 200 and 290 days after the experiment start and their oral mucosa studied histochemically and ultrastructurally. In the alcoholic animal, 1) the epithelium was thickened from day 98 on and SS and SH groups were detected in the keratin; 2) at this time interval, positive acid phosphatase areas were noted. Under the electron microscope, myelin figures were observed phosphatase areas were noted. Under the electron microscope, myelin figures were observed between cells on days 98 and 200. Cells undergoing autolysis could explain the lysosomial enzyme activity; 3) the DNA activity assessed by methyl-pyronine green stain was increased after 200 and 290 days. This activity was correlated at these time intervals with the increase of the mitosis number; 4) succinodeshydrogenase showed an increased activity on days 200 and 290. The mitochondria often of large size showed no ultrastructural changes even on day 290; 5) the vessels, already on day 98, then on days 200 and 290, showed a decrease in alkaline phosphatase reactivity with occasional thinnings of the wall. These modifications suggested that alcohol was able to modify the integrity of oral mucosa cells in the rat, perhaps by modifying the quality of the cell walls.
D000284:Administration, Oral; D000818:Animals; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006651:Histocytochemistry; D009061:Mouth Mucosa / Q000187:drug effects* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000431:Ethanol
Etude histochimique et ultrastructurale de la muqueuse buccale du rat, après administration chronique d'alcool.