Detection of hybrid (combinatorial) Ia antigens using parent anti-F1 sera.
Ia specificities 22 and 23 were initially identified by using conventional alloantisera and were mapped to the I-E subregion of k and d haplotypes on the basis of their reactivity with selected recombinants. Recently we found that Ia 22 and 23 are hybrid determinants on the basis of their expression on selected F1 cells, but absence from both parental cells. Initial attempts to detect hybrid Ia antigens by immunizing parents with F1 cells were unsuccessful. By utilizing lipopolysaccharide (LPS)-stimulated F1 spleen lymphoblasts as immunogens, 1 of the parents as recipient and the other parent cells for absorption of antisera, specific anti-Ia.22 and 23 antibodies were produced. The specificity of these parent anti-F1 sera was confirmed by cytotoxic and immunoprecipitation analyses.
D000042:Absorption; D000818:Animals; D011232:Chemical Precipitation; D003433:Crosses, Genetic; D000949:Histocompatibility Antigens Class II; D006822:Hybrid Cells / Q000276:immunology*; D007106:Immune Sera / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains
D000949:Histocompatibility Antigens Class II; D007106:Immune Sera; D008070:Lipopolysaccharides