title: Periodic synthesis of microtubular proteins in the cell cycle of Physarum.
abstract: Periodic polypeptide labeling over the naturally synchronous nuclear replication cycle of Physarum polycephalum was analyzed by fluorography of two-dimensional electropherograms. Two sets of polypeptides, denoted as P and Q, showed strong periodicity; they were maximally labeled just prior to mitosis. This periodicity was shown to reflect synthesis rather than turnover or recovery. Both P and Q copolymerized with porcine microtubular proteins and displayed electrophoretic properties similar to those of porcine tubulins. The significance of the periodic synthesis of these microtubular proteins is discussed as a possible component within the chain of events that establishes the high mitotic synchrony of Physarum syncytia.
mesh_terms: D000818:Animals; D007700:Kinetics; D008870:Microtubules / Q000378:metabolism*; D008938:Mitosis; D010507:Periodicity; D010804:Physarum / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D011485:Protein Binding; D013552:Swine; D014404:Tubulin / Q000096:biosynthesis*
chemical_list: D014404:Tubulin