Gamma-irradiation activates biochemical systems: induction of nitrate reductase activity in plant callus.
Gamma-irradiation induced high levels of nitrate reductase activity (NADH:nitrate oxidoreductase, EC 1.6.6.1) in callus of Haworthia mirabilis Haworth. Subcultures of gamma-irradiated tissues showed autonomous growth on minimal medium. We were able to mimic the effects of gamma-irradiation by inducing nitrate reductase activity in unirradiated callus with exogenous auxin and kinetin. These results revealed that induction of nitrate reductase activity by gamma-irradiation is mediated through in vitro activation of hormone synthesis in callus cells.
D002478:Cells, Cultured; D005720:Gamma Rays; D007294:Inositol / Q000494:pharmacology; D009565:Nitrate Reductases / Q000528:radiation effects*; D010937:Plant Growth Regulators / Q000494:pharmacology; D010944:Plants / Q000201:enzymology* / Q000528:radiation effects
D010937:Plant Growth Regulators; D007294:Inositol; D009565:Nitrate Reductases