Isolation and characterization of peptide YY (PYY), a candidate gut hormone that inhibits pancreatic exocrine secretion.
A new candidate hormone--designated peptide YY (PYY)--has been isolated from extracts of porcine upper intestinal tissue by using a novel chemical assay method. This peptide has been detected in the extracts by the presence of its unusual COOH-terminal tyrosine amide structure. The peptide consists of a linear chain of 36 amino acids and its complete amino acid sequence is: Tyr-Pro-Ala-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Ser-Arg-Tyr -Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-NH2. This peptide has a distinct sequence homology to the pancreatic polypeptide. It is therefore proposed that PYY and the pancreatic polypeptide together form a new peptide family. PYY has been found to strongly inhibit both secretin- and cholecystokinin-stimulated pancreatic secretion in the anesthetized cat.
D000595:Amino Acid Sequence; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D001681:Biological Assay; D002415:Cats; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002918:Chymotrypsin; D007421:Intestine, Small / Q000032:analysis*; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D010446:Peptide Fragments / Q000302:isolation & purification; D019894:Peptide YY; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D013552:Swine; D014357:Trypsin
D001639:Bicarbonates; D010446:Peptide Fragments; D010455:Peptides; D019894:Peptide YY; D002918:Chymotrypsin; D014357:Trypsin