Electron microscope study of duck globin mRNA precursor crosslinked in situ.
Double-stranded RNA segments present in duck globin pre-mRNA were crosslinked in situ with aminomethyltrioxalen and UV light. The secondary structure of the crosslinked pre-mRNA was then studied by electron-microscopic analysis of pre-mRNA . cDNA hybrids. The data suggest that duck globin pre-mRNAs contain intervening sequences that are excised stepwise. Excision and subsequent ligation appears to occur on precursor molecules that are stabilized by base-paired regions.
D000818:Animals; D003432:Cross-Linking Reagents; D004372:Ducks / Q000235:genetics*; D005914:Globins / Q000235:genetics*; D006860:Hydrogen Bonding; D008854:Microscopy, Electron; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D009694:Nucleic Acid Precursors / Q000235:genetics*; D012332:RNA, Heterogeneous Nuclear / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D014307:Trioxsalen
D003432:Cross-Linking Reagents; D009694:Nucleic Acid Precursors; D012332:RNA, Heterogeneous Nuclear; D012333:RNA, Messenger; D005914:Globins; D014307:Trioxsalen