Podophyllotoxin resistance: a codominant selection system for quantitative mutagenesis studies in mammalian cells.
Mutants resistant to the microtubule inhibitor podophyllotoxin (PodR), a codominant marker, can be readily selected in various mammalian cell lines such as, CHO, HeLa, mouse L cells, Syrian hamster cells (BHK21) and a mouse teratocarcinoma cell line OC15. In CHO cells, the recovery of PodR mutants is not affected by cell density (up to 1 X 10(6) cells per 100-mm diameter dish), and after treatment with the mutagen ethyl methanesulfonate maximum mutagenic effect is achieved after a relatively short expression time (40-48 h). The frequency of PodR mutants in various cell lines increased in a dose-dependent manner in response to treatment with the mutagens ethyl methanesulfonate and N-methyl-N'-nitro-N-nitrosoguanidine. The PodR selection system thus provides a new genetic marker which should prove useful in studies of quantitative mutagenesis in mammalian cells.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D005260:Female; D005819:Genetic Markers; D006367:HeLa Cells; D006801:Humans; D007668:Kidney; D007739:L Cells; D008647:Mesocricetus; D051379:Mice; D009154:Mutation; D010053:Ovary; D011034:Podophyllotoxin / Q000494:pharmacology*; D013724:Teratoma
D005819:Genetic Markers; D011034:Podophyllotoxin