Giemsa-11 technique. Applications in the chromosomal characterization of hematologic specimens.
Use of the Giemsa-11 procedure for the localization of heterochromatic regions of human chromosomes and for differentiation of primate and rodent chromosomes has been somewhat limited since its discovery in 1972. An adaptation of this technique to the cytogenetic characterization of hematologic specimens has aided in the interpretation of translocations, deletions, and inversions involving human chromosome 9. The chromosomal analyses of 10% of over 100 patients, principally leukemic, were aided through the use of this auxiliary procedure. The diseases of these patients are given and portions of karyotypes are presented to show clarification of abnormalities made possible through the use of the Giemsa-11 technique.
D001399:Azure Stains; D001773:Blood Cells / Q000648:ultrastructure; D001853:Bone Marrow / Q000473:pathology; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002872:Chromosome Deletion; D007446:Chromosome Inversion; D002906:Chromosomes, Human, 6-12 and X; D006801:Humans; D007621:Karyotyping; D007938:Leukemia / Q000235:genetics*; D007945:Leukemia, Lymphoid / Q000235:genetics; D007948:Leukemia, Monocytic, Acute / Q000235:genetics; D007951:Leukemia, Myeloid / Q000235:genetics; D015470:Leukemia, Myeloid, Acute / Q000235:genetics; D010640:Phenothiazines; D014178:Translocation, Genetic
D001399:Azure Stains; D010640:Phenothiazines