Protein-methotrexate-IgG complexes in the serum of patients receiving high-dose antifolate therapy.
Three patients who were treated repetitive courses of high-dose methotrexate and leucovorin rescue experienced hypersensitivity symptoms infusion of the drug. Serum from all three patients contained one complex of methotrexate bound to a protein similar in size to albumin and a second complex of the protein-bound methotrexate coupled to IgG. This immune complex was not found in 4 patients similarly treated who did not experience hypersensitivity symptoms.
D000936:Antigen-Antibody Complex; D001943:Breast Neoplasms / Q000188:drug therapy; D002850:Chromatography, Gel; D004334:Drug Administration Schedule; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000209:etiology; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology*; D007263:Infusions, Parenteral; D008727:Methotrexate / Q000008:administration & dosage* / Q000097:blood / Q000276:immunology; D012516:Osteosarcoma / Q000188:drug therapy; D011485:Protein Binding
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D008727:Methotrexate