Indirect induction of mutagenesis of intact parvovirus H-1 in mammalian cells treated with UV light or with UV-irradiated H-1 or simian virus 40.
The frequency of mutants among the descendants of intact parvovirus H-1 was increased when cells of human and rat origin were exposed to UV light prior to infection. An enhanced mutagenesis of intact H-1 could be induced to almost the same extent in unirradiated cells by preinfecting them with H-1 or simian virus 40 that had been irradiated with UV light. The expression of an enhanced mutagenesis of unirradiated H-1 after cell treatment with UV light or UV-irradiated virus was delayed, transient, and dose-dependent, and it was dependent on de novo protein synthesis.
D000818:Animals; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009154:Mutation / Q000528:radiation effects*; D010321:Parvoviridae / Q000235:genetics*; D051381:Rats; D014466:Ultraviolet Rays; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000528:radiation effects
D003513:Cycloheximide