Identification of the 3-sulfate isomer as the major product of enzymatic sulfation of chenodeoxycholate conjugates.
Thin layer and high performance liquid chromatography identified the 3-monosulfate ester as the predominant product of in vitro enzymatic sulfation of glyco- and taurochenodeoxycholate by rat liver and kidney and hamster liver. The rate of synthesis of the 7-sulfate ester was less than 20% that of the 3-sulfate isomer; in vitro synthesis of the 3,7-disulfate was not definitely seen. Reaction of the enzymatic products with 7 alpha-hydroxysteroid dehydrogenase indicated a molar ratio of 7 alpha-hydroxyl function and SO4 which further supported the identification of the 3-sulfate isomer as the major product.
D000818:Animals; D002635:Chenodeoxycholic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006224:Cricetinae; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D051381:Rats; D013466:Sulfurtransferases / Q000378:metabolism*
D002635:Chenodeoxycholic Acid; C027616:chenodeoxycholate sulfate conjugate; D006913:Hydroxysteroid Dehydrogenases; C025376:7 alpha-hydroxysteroid dehydrogenase; D013466:Sulfurtransferases