Lysozyme binding by a polyglycerol phosphate polymer of the oral bacterium Streptococcus mutans BHT.
The antibacterial properties of lysozyme for Streptococcus mutans BHT may be a function of its binding to cell components other than to peptidoglycan. Inhibitors of muramidase activity, including histamine and N-acetyl-D-glucosamine, only partially blocked the bacteriostatic effects on this strain. Greater than 20 mM histamine alone inhibited growth suggesting a bacteriostatic potential. An autoclaved saline extract was then prepared from stationary phase cultures in a chemically-defined medium. As little as 31.25 micrograms of the extract significantly blocked the effect of 50 micrograms lysozyme and complete enzyme inhibition was achieved with 62.5 micrograms. The extract was fractionated and location of potential binding components determined by a precipitin method consisting of diffusing the samples into 1.2 per cent agarose containing lysozyme. Binding components eluted in the first peak of a Sephacryl S-300 column, bound to DEAE-cellulose, but desorbed with gradient elution (0.1-1.0 M tris-HCl buffer, pH 8.0). The eluted material was then applied to an affinity column containing purified lysozyme coupled to epoxy-activated Sepharose 6B. Non-absorbed anionic material precipitated only with protamine. Lysozyme-binding fractions eluted in a sharp peak with 1.0 M tris-HCl buffer (pH 8.0), did not bind wheat-germ agglutinin, contained less than 50 micrograms protein, 95 micrograms sugar, 66.7 micrograms phosphorus, less than 0.25 mequiv lipid and no detectable nucleic acids. The peak material reacted with antiserum directed against polyglycerol phosphate, indicating that it contained acylated or, possibly, deacylated lipoteichoic acid. The findings suggest that the antibacterial properties of lysozyme for Strep. mutans BHT may, in part, be modified (or possibly regulated) by binding to molecules such as lipoteichoic acid.
D005994:Glycerophosphates / Q000378:metabolism*; D008070:Lipopolysaccharides; D009113:Muramidase / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000494:pharmacology; D010712:Phosphatidic Acids / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D011485:Protein Binding; D013295:Streptococcus mutans / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D013682:Teichoic Acids / Q000378:metabolism
D005994:Glycerophosphates; D008070:Lipopolysaccharides; D010712:Phosphatidic Acids; D011135:Polysaccharides, Bacterial; D013682:Teichoic Acids; C008964:polyglycerolphosphate; C009900:lipoteichoic acid; D009113:Muramidase