Absence of effect of prostaglandins on cholesteryl ester metabolism of 3T3 mouse fibroblasts grown in tissue culture.
This study examines the effect of prostaglandin E2 and 6-keto F1 alpha on the cholesteryl ester metabolism of cells grown in tissue culture. When 3T3 mouse fibroblasts were incubated with cationized low density lipoprotein (LDL) and 3H-labelled oleic and 14C-labelled linoleic acids a marked increase in cholesteryl ester content of cells was observed. Oleic acid was the preferred substrate for cholesterol esterification. However, the presence of prostaglandin E2 or 6-keto F1 alpha (up to 10 micrograms/ml) did not affect the cholesteryl ester content or the uptake of labelled fatty acids into cellular lipids. Following preincubation with cationized LDL and labelled fatty acids the cells were reincubated in normal medium with or without prostaglandins. The presence of PGE2 or 6KPGF1 alpha (up to 10 micrograms/ml) did not appreciably change the rate of removal of cholesteryl ester or labelled lipids. This indicates that these prostaglandins even when present in relatively large doses in the incubation medium do not affect lipid metabolism of cells grown in tissue culture.
D015121:6-Ketoprostaglandin F1 alpha / Q000494:pharmacology; D000818:Animals; D002250:Carbon Radioisotopes; D002788:Cholesterol Esters / Q000378:metabolism*; D015232:Dinoprostone; D005347:Fibroblasts; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011453:Prostaglandins / Q000494:pharmacology*; D011458:Prostaglandins E / Q000494:pharmacology; D014280:Triglycerides / Q000378:metabolism; D014316:Tritium
D002250:Carbon Radioisotopes; D002788:Cholesterol Esters; D008041:Linoleic Acids; D008077:Lipoproteins, LDL; D009829:Oleic Acids; D010743:Phospholipids; D011453:Prostaglandins; D011458:Prostaglandins E; D014280:Triglycerides; D014316:Tritium; D019301:Oleic Acid; D015121:6-Ketoprostaglandin F1 alpha; D019787:Linoleic Acid; D015232:Dinoprostone