A new method for permanent preparations of hemopoietic cells cultured in methylcellulose medium.
To obtain permanent preparations for morphologic examination of hemopoietic cells cultured in methylcellulose medium, a new method designated as "the membrane filtration technique" has been devised. Permanent preparations of erythroid colonies, bursts and leukemic colonies, which have been grown mainly in methylcellulose medium, are illustrated in this paper. The stages of differentiation of cells within these colonies can be easily recognized by means of Wright-Giemsa staining.
D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media; D004920:Erythropoiesis; D005374:Filtration / Q000295:instrumentation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology; D008747:Methylcellulose / Q000494:pharmacology*; D010969:Plastics; D011108:Polymers
D003470:Culture Media; D010969:Plastics; D011108:Polymers; C017902:lexan; D008747:Methylcellulose