An 18,200-dalton adrenal protein that contains four [Met]enkephalin sequences.
An 18,200-dalton adrenal enkephalin-containing polypeptide was purified to homogeneity and shown to contain four internal [Met]enkephalin sequences. Protein sequence analysis and mapping of trypsin-derived peptides demonstrated that the previously described 12,600-dalton and 5,300-dalton adrenal enkephalin-containing polypeptides compose the amino-terminal and carboxyl-terminal sequences, respectively, of this larger protein. They are linked by two basic amino acid residues to form the entire sequence of the 18,200-dalton protein. This protein is the largest enkephalin-containing polypeptide yet isolated from beef adrenal medulla and serves as the precursor for several other previously characterized adrenal enkephalin-containing proteins and peptides.
D000313:Adrenal Medulla / Q000032:analysis*; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D004723:Endorphins / Q000378:metabolism*; D004745:Enkephalins / Q000378:metabolism*; D008970:Molecular Weight; D011498:Protein Precursors / Q000302:isolation & purification / Q000378:metabolism*
D004723:Endorphins; D004745:Enkephalins; D011498:Protein Precursors