Biosynthesis of rat enamel matrix components in vivo.
The biosynthesis of enamel matrix components of developing rat incisors was investigated by measuring the incorporation of 3 H-proline, 32P-phosphate, and 35S-sulfate in vivo. 3H- and 32P-radioactivity was found in what seemed to be a prototype of enamel proteins. Subsequent shifts in other protein and peptide fractions were observed. 35S was also incorporated into components other than the above-mentioned proteins.
D000818:Animals; D003743:Dental Enamel / Q000378:metabolism*; D003746:Dental Enamel Proteins / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D007180:Incisor; D008297:Male; D010710:Phosphates / Q000378:metabolism; D010766:Phosphorylation; D011392:Proline / Q000378:metabolism; D011868:Radioisotopes; D051381:Rats; D011919:Rats, Inbred Strains; D013431:Sulfates / Q000378:metabolism; D013997:Time Factors
D003746:Dental Enamel Proteins; D010710:Phosphates; D011868:Radioisotopes; D013431:Sulfates; D011392:Proline