Morphochemical study of brown adipose tissue during adrenal gland regeneration in the white rat.
The interscapular brown adipose tissue in male, white Wistar rats in the 1,3,6,15,30, and 60th day of regeneration of adrenals after their enucleation weas investigated. The tissue slides were stained with H and E, with Masson's triple stain and with Sudan B. The activity of the following dehydrogenses was evaluated: Succinic dehydrogenase, delta 5,3 beta-hydroxysteroid dehydrogenase and the activity of the following enzymes; monoaminooxidase (MAO), Alkaline phosphatase (AlPh) and acid phosphatase (AcPh) was studied. Between the first ad 30th day of regeneration of adrenals morphochemical changes were observed; a great number of cells with eosinophylic cytoplasm appeared and with minute vacuols in their cytoplasm, the blood vessels became more numerous and enlarged and the activity of the enzymes tested was enhanced, instead sudanophilia was weaken and the reaction of corticosteriods significantly intensified. The changes observed may indicate on an enhanced hormonal activity. The brown adipose tissue may function as an endocrine gland which supports the regenerating adrenals of the white rat, enables survival and preservation of homeostasis during the insufficiency of the adrenal cortex after adrenal enucleation. The initiation of the endocrine activity of the brown adipose tissue in the course of regeneration of the adrenals proceeds probably without the direct participation of catecholamines originating from the adrenals.
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism; D002001:Adipose Tissue, Brown / Q000032:analysis / Q000166:cytology*; D000305:Adrenal Cortex Hormones / Q000032:analysis; D000311:Adrenal Glands / Q000502:physiology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D008055:Lipids / Q000032:analysis; D008297:Male; D008995:Monoamine Oxidase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012038:Regeneration; D013385:Succinate Dehydrogenase / Q000378:metabolism
D000305:Adrenal Cortex Hormones; D008055:Lipids; D015096:3-Hydroxysteroid Dehydrogenases; C031954:delta(5)-3 beta-hydroxysteroid dehydrogenase; D013385:Succinate Dehydrogenase; D008995:Monoamine Oxidase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase