Purification of 20 alpha-hydroxysteroid dehydrogenase from human erythrocytes.
20 alpha-hydroxysteroid dehydrogenase was found in the supernatant after hemolysis of human erythrocytes, and the enzyme was isolated from the membrane-free hemolysate on a DEAE-cellulose column. The NADPH-generating system was essential for the reaction.
D015089:20-Hydroxysteroid Dehydrogenases / Q000097:blood* / Q000302:isolation & purification; D002855:Chromatography, Thin Layer; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans
D015089:20-Hydroxysteroid Dehydrogenases