title: On the production of 12 alpha-hydroxysteroid dehydrogenase from Clostridium group P, strain C48-50 ATCC 29733.
abstract: The production of 12 alpha-hydroxysteroid dehydrogenase of Clostridium group P strain C48-50 was optimized when the organism was grown in the presence of 2% fructose and 0.1% dithiothreitol. It appears that an initial redox potential of less than -160 mV (achieved by autoclaving in the presence of dithiothreitol, dithioerythritol or cysteine) is important in the production of this enzyme.
mesh_terms: D002793:Cholic Acids / Q000096:biosynthesis; D003013:Clostridium / Q000201:enzymology*; D004229:Dithiothreitol / Q000494:pharmacology; D004790:Enzyme Induction; D005632:Fructose / Q000494:pharmacology; D006913:Hydroxysteroid Dehydrogenases / Q000096:biosynthesis*; D010084:Oxidation-Reduction
chemical_list: D002793:Cholic Acids; D005632:Fructose; D006913:Hydroxysteroid Dehydrogenases; C019189:12 alpha-hydroxysteroid dehydrogenase; D004229:Dithiothreitol