Detection of HLA-D region products by primed lymphocyte typing (PLT).
In vitro priming experiments were performed with lymphocytes from members of two different families carrying Dw-,DR2 or Dw2,DR2 haplotypes. It was demonstrated that lymphocytes could be primed to allogeneic HLA-D determinants without detectable priming to the associated HLA-DR determinant, even when the priming cell was also HLA-DR incompatible to the responding cell. It was further shown that the unknown HLA-D determinants of the two families (e.g., Dw-) were different, one of them showing cross-reactivity to Dw2. Priming to MT1 determinants or to Lewis antigens could not be detected.
D002874:Chromosome Mapping; D003429:Cross Reactions; D005260:Female; D008040:Genetic Linkage; D006238:Haploidy; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007983:Lewis Blood Group Antigens; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D010375:Pedigree; D014176:Protein Biosynthesis
D000949:Histocompatibility Antigens Class II; D007983:Lewis Blood Group Antigens