Somatic cell genetic analysis of regulation of expression of 3-hydroxy-3-methylglutaryl-coenzyme A reductase.
Three new types of regulatory somatic cell mutants defective in expression of the enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase are described. They include a recessive mutant with abnormally high enzyme activity, apparently defective in degradation of the enzyme, and one, phenotypically a mevalonate auxotroph, that maintains permanently repressed levels of enzyme activity and are recessive, constitutive mutants. These mutants offer means for analyses of the mechanism of regulation of expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase in cultured fibroblasts.
D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol / Q000494:pharmacology; D006224:Cricetinae; D004351:Drug Resistance; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006888:Hydroxycholesterols / Q000494:pharmacology; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics*; D008798:Mevalonic Acid / Q000378:metabolism; D010053:Ovary
D006888:Hydroxycholesterols; D002784:Cholesterol; D006903:Hydroxymethylglutaryl CoA Reductases; D008798:Mevalonic Acid