Determination of 5-hydroxytryptamine in rat brain regions by gas-liquid chromatography with electron-capture detection.
An assay method for measurement of 5-hydroxytryptamine in rat brain areas is described. The procedure involves extraction with a liquid ion exchanger followed by acetylation and reaction with pentafluoropropionic anhydride. The resultant beta-carboline is separated and quantitated using gas-liquid chromatography with electron-capture detection. The procedure also works well with 5-methoxytryptamine standards, and the tissue samples were checked simultaneously for the presence of this amine. The results indicate that concentrations of 5-methoxytryptamine in all areas studied (brain stem, hypothalamus, frontal cortex, and striatum) are less than 10 ng/g.
D008735:5-Methoxytryptamine / Q000032:analysis; D000107:Acetylation; D000818:Animals; D001923:Brain Chemistry; D002849:Chromatography, Gas / Q000379:methods; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D012701:Serotonin / Q000032:analysis*
D012701:Serotonin; D008735:5-Methoxytryptamine