DNA synthesis in thymic-acute lymphoblastic leukaemia.
Deoxyribonucleoside triphosphate (dNTP) concentrations were measured in bone marrow and peripheral blood leucocytes from seven patients with acute Thy-lymphoblastic leukaemia (Thy-ALL), 12 patients with acute myeloblastic leukaemia and 15 patients with acute non-T, non-B lymphoblastic leukaemia (c-ALL), and in thymocytes from patients with myasthenia gravis. Labelled thymidine and deoxycytidine incorporation into DNA was also studied. In Thy-ALL, dNTP concentrations were markedly increased compared with those in the other acute leukaemias. The dNTP concentrations in thymocytes were, however, similar to those in Thy-ALL. 3H-nucleoside incorporation studies showed a marked difference in labelled deoxycytidine incorporation and particularly in the deoxycytidine/thymidine DNA labelling ratio between Thy-ALL and the other cell types. We conclude that the pathways of DNA synthesis in Thy-ALL blasts are different from those in the cells from acute acute leukaemias and some but not all these differences may correspond to differences between normal cortical thymocytes and bone marrow cells.
D001853:Bone Marrow / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D003841:Deoxycytidine / Q000378:metabolism; D003853:Deoxyribonucleosides / Q000378:metabolism; D006801:Humans; D007945:Leukemia, Lymphoid / Q000378:metabolism*; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism; D009157:Myasthenia Gravis / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism
D004273:DNA, Neoplasm; D003853:Deoxyribonucleosides; D003841:Deoxycytidine; D004247:DNA; D013936:Thymidine