Inactive X chromosome DNA does not function in DNA-mediated cell transformation for the hypoxanthine phosphoribosyltransferase gene.
The molecular nature of the X chromosome inactivation process has been investigated by utilizing the techniques of DNA-mediated cell transformation of the X-linked hypoxanthine phosphoribosyltransferase (HPRT) locus. The findings indicate that purified DNA from the inactive X chromosome of a near-euploid mouse cell line is not functional in transformation for HPRT, but the DNA from its "homologous" active X readily elicits transformation for HPRT in the same hamster cell recipient. These findings suggest that there is a difference between the DNA, per se, of the active and inactive X at (or near) the HPRT locus and that this difference could account, at least in part, for its inactivation.
D000483:Alleles; D000818:Animals; D002460:Cell Line; D004247:DNA / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D005796:Genes; D008040:Genetic Linkage; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D051379:Mice; D012730:Sex Chromosomes; D014170:Transformation, Genetic; D014960:X Chromosome
D004247:DNA; D007041:Hypoxanthine Phosphoribosyltransferase