Urinary excretion of arterial blood prostaglandins and thromboxanes in man.
To determine the fraction of the arterial prostaglandin E2 (PGE2), 6-ketoprostaglandin F1 alpha (6-keto-PGF1 alpha), and thromboxane B2 (TXB2) that is excreted unmetabolized into human urine, the 3H-labeled compounds were separately infused into the renal artery or brachial vein of 23 subjects. Urine extracts were subjected to sequential chromatography on thin-layer plates, Sephadex LH-20, and reverse-phase high-pressure liquid chromatography to isolate the unmetabolized fraction. Dual isotope ratio techniques were used to identify peak fractions, to assess purity, and to calculate recovery. Following renal artery infusions, 32.2% of 6-keto-PGF1 alpha, 13.5% of TXB2, and 3.9% of PGE2 were excreted unmetabolized. Calculated fractional excretion of these compounds on a single transit through the kidney are approximately 30, 13, and 3.9%, respectively. Following brachial vein infusion of [3H]prostaglandin I2, 2.7% of the infusate was excreted as 6-keto-PGF1 alpha, suggesting that circulating prostaglandin I2 may contribute to urinary 6-keto-PGF1 alpha. When combined with published measurements of urinary PGE2, 6-keto-PGF1 alpha, and TXB2, these data can be used to calculate the maximum arterial blood concentration of these substances. The results indicate that arterial blood concentration of PGE2, TXB2, and 6-keto-PGF1 alpha in man are only a few picograms per milliliter or less.
D000328:Adult; D000368:Aged; D002845:Chromatography; D002855:Chromatography, Thin Layer; D015232:Dinoprostone; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011458:Prostaglandins E / Q000008:administration & dosage / Q000097:blood / Q000652:urine*; D011460:Prostaglandins F / Q000008:administration & dosage / Q000097:blood / Q000652:urine*; D012016:Reference Values; D013929:Thromboxane B2 / Q000008:administration & dosage / Q000097:blood / Q000652:urine*; D013931:Thromboxanes / Q000652:urine*
D011458:Prostaglandins E; D011460:Prostaglandins F; D013931:Thromboxanes; D013929:Thromboxane B2; D015232:Dinoprostone; C100006:prostaglandin F1