title: Effect of nicotine on the formation of prostaglandins in the rabbit kidney.
abstract: The effect of nicotine on the bioformation of prostaglandins (PG) in the rabbit kidney was investigated. Rabbit kidneys were homogenized and the low-speed supernatant was incubated with 14C-labelled arachidonic acid (14C-AA), a PG precursor. Nicotine was added to the incubations to produce final concentrations of 5 x 10(-7) M to 5 x 10(-4) M. Control incubations without nicotine were also performed. 14C-PG formed were separated and evaluated on thin layer radiochromatography. In control experiments 14C-labelled PGD2, PGE2 and PGF2 were formed, the relative amounts in between them being 16, 31 and 53%, respectively. Nicontine dose-dependently depressed the formation of all PG formed in the kidney. At 5 x 10(-7) M the formation of 14C-PG was depressed to 45-80% of control and at a concentration of 5 x 10(-4) M it was depressed to 40% or less of control. It is concluded that nicotine elicits an overall depressive effect on the rabbit kidney formation of PG, probably by inhibiting cyclo-oxygenation of AA.
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009538:Nicotine / Q000494:pharmacology*; D011453:Prostaglandins / Q000096:biosynthesis*; D011817:Rabbits
chemical_list: D001095:Arachidonic Acids; D011453:Prostaglandins; D009538:Nicotine