Identification of a 'urinary-type' metabolite of prostaglandin F2 alpha in the rat circulation.
During constant slow intravenous infusion of tritiated prostaglandin F2 alpha into male adult rats, a major portion of radioactivity in blood appeared as a new metabolite, identified as tetranor-15-keto-13,14-dihydroprostaglandin F2 alpha (18.8 +/- 4.2%, n = 7). The previously recognised blood metabolite, 15-keto-13,14-dihydroprostaglandin F2 alpha, was also observed (15.1 +/- 4.1%, n = 7). 15-keto-13,14-Dihydroprostaglandin F2 alpha disappeared quickly from the circulation while tetranor-15-keto-13,14-dihydroprostaglandin F2 alpha was still detected (8.6 +/- 2.8%, n = 3) 2 h after infusion was stopped. These results indicate that tetranor-15-keto-13,14-dihydroprostaglandin F2 alpha, because of its slow disappearance from the circulation, may provide a better indicator than 15-keto-13,14-dihydroprostaglandin F2 alpha of prostaglandin F2 alpha synthesis in vivo.
D000818:Animals; D015237:Dinoprost; D005227:Fatty Acids / Q000097:blood*; D008297:Male; D011460:Prostaglandins F / Q000378:metabolism; D011466:Prostanoic Acids / Q000097:blood*; D051381:Rats
D005227:Fatty Acids; D011460:Prostaglandins F; D011466:Prostanoic Acids; C019171:prostaglandin F-main urinary metabolite; D015237:Dinoprost