Gel filtration separation of bound and free antigens in radioimmunoassay of prostaglandin F2 alpha.
A technique applying the principles of gel filtration to separate the antibody-bound antigen for radioimmunoassay of PGF2 alpha is described. The procedure involves addition of the entire assay incubate to a column of Sephadex G-75 and elution of the antibody-bound fraction directly into a scintillation vial. Subsequent rinsing elutes the free fraction enabling the column to be re-used. The validity of this method for the direct measurement of PGF2 alpha in a standard tissue culture medium is demonstrated and its advantages over other separation techniques discussed.
D000941:Antigens / Q000302:isolation & purification; D002850:Chromatography, Gel / Q000379:methods; D015237:Dinoprost; D011460:Prostaglandins F / Q000032:analysis* / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods
D000941:Antigens; D011460:Prostaglandins F; D015237:Dinoprost