Synthesis of thymosin beta 4 by peritoneal macrophages and adherent spleen cells.
Thymosin beta 4, previously identified as a component of thymosin fraction 5 isolated from calf thymus, is synthesized by peritoneal macrophages derived from rats or mice, including cells from nu/nu mice. Identification of thymosin beta 4 containing [35S]methionine was based on its retention time in reverse-phase HPLC and the recovery of radioactivity in peptides generated by mild acid hydrolysis or by digestion with trypsin. Thymosin beta 4 is  also synthesized by rat and mouse spleen cells, and evidence is presented for its release from these cells.
D000818:Animals; D002448:Cell Adhesion; D008214:Lymphocytes / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013154:Spleen / Q000378:metabolism; D013947:Thymosin / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000302:isolation & purification; D013951:Thymus Hormones / Q000096:biosynthesis*; D000099201:Thymosin beta(4)
D013947:Thymosin; D013951:Thymus Hormones; D000099201:Thymosin beta(4)