title: Turnover of nitrogenase and leghemoglobin in root nodules of Pisum sativum.
abstract: Turnover rates of the two nitrogenase components and leghemoglobin in root nodules of pea plants nodulated with Rhizobium leguminosarum were determined with three different methods: 1, Kinetics of 35S incorporation into protein; 2, pulse-chase experiments; 3, chloramphenicol inhibition of bacteroid protein synthesis. Methods 1 and 3 revealed that the turnover rates of the two nitrogenase components and leghemoglobin are identical to the average rate of bacteroid and plant nodule protein turnover. The t1/2 times of component I and II and leghemoglobin were about 2 days. Pulse-chase experiments with 35SO(2-)4 appeared to be rather unsuitable for determination of turnover rates in pea root nodules.
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002701:Chloramphenicol / Q000494:pharmacology; D007887:Fabaceae / Q000378:metabolism*; D006420:Hemeproteins / Q000378:metabolism*; D007700:Kinetics; D007874:Leghemoglobin / Q000378:metabolism*; D009591:Nitrogenase / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D010946:Plants, Medicinal; D012231:Rhizobium / Q000187:drug effects / Q000378:metabolism*; D013559:Symbiosis
chemical_list: D001426:Bacterial Proteins; D006420:Hemeproteins; D007874:Leghemoglobin; D010940:Plant Proteins; D002701:Chloramphenicol; D009591:Nitrogenase