title: Molecular cloning of cDNA coding for the gamma subunit of Torpedo acetylcholine receptor.
abstract: From the electric organ of Torpedo californica, we purified mRNA that, when translated in vitro, produces polypeptides immunoprecipitable by antibodies against purified acetylcholine receptor. A novel cloning system [Okayama, H. & Berg, P. (1982) Mol. Cell. Biol. 2, 161-170] was used to produce a cDNA library from this mRNA. This library contained clones with receptor sequences identified by differential hybridization and hybridization-selection. We describe a clone of 2,030 base pairs with sequences appropriate for the amino-terminal amino acids of the gamma subunit of acetylcholine receptor. This clone contains 82 bases 5' of the codon for the amino-terminal amino acid of the mature protein. A portion of this sequence codes for a methionine followed by a 16-amino acid polypeptide that is contiguous to the amino-terminal amino acid of the mature protein and that has the characteristics of a leader peptide. The cDNA insert hybridizes to a 2,100-base RNA present in electric organ but not in the brain of T. californica.
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004557:Electric Organ / Q000032:analysis; D046911:Macromolecular Substances; D008970:Molecular Weight; D012333:RNA, Messenger / Q000302:isolation & purification; D011950:Receptors, Cholinergic / Q000235:genetics*; D014101:Torpedo
chemical_list: D046911:Macromolecular Substances; D012333:RNA, Messenger; D011950:Receptors, Cholinergic; D004247:DNA