Purification of thymus mRNA coding for a 16,000-dalton polypeptide containing the thymosin alpha 1 sequence.
A mRNA fraction purified by preparative polyacrylamide disc gel electrophoresis from calf thymus polysomes codes for a polypeptide(s) having a mass of 16,000-17,000 daltons. This polypeptide contains amino acid sequences corresponding to residues 11-18 and 19-25 of thymosin alpha 1. The yield of the octapeptide indicates that the 16,000-dalton peptide is the major product formed in the cell-free synthesis system containing the purified mRNA.
D000818:Animals; D002417:Cattle; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D011132:Polyribosomes; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000302:isolation & purification*; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives / Q000235:genetics*; D013950:Thymus Gland / Q000032:analysis; D013951:Thymus Hormones / Q000235:genetics*
D011498:Protein Precursors; D012333:RNA, Messenger; D013951:Thymus Hormones; D013947:Thymosin; D000077596:Thymalfasin