Iron staining of the acquired enamel pellicle after exposure to tannic acid or chlorhexidine: preliminary report.
Extrinsic discoloration of teeth following a large consumption of tannin-containing beverages or a prolonged use of chlorhexidine mouthrinses is a well known observation. Tannins as well as chlorhexidine are denaturing agents. Based on preliminary studies revealing the presence of iron in chlorhexidine discolored pellicle material, the ability of iron to stain the integument after pretreatment with the two denaturants was studied in a human model. The denaturing effect of an acidic environment was also included. Enamel slabs fixed to acrylic appliances were carried in the oral cavity and alternately exposed to the test solutions in different sequences in vitro. Pretreatment with chlorhexidine or tannic acid led to marked discoloration upon iron application during 5-d tests, whereas the compounds individually had no such effect. A large content of the metal was found in the stained material. Stannous fluoride appeared to reduce the formation of the pigments, and strong oxidation completely bleached the established color. Possible mechanisms underlying the phenomena observed are discussed.
D002710:Chlorhexidine / Q000494:pharmacology*; D003741:Dental Deposits / Q000032:analysis / Q000503:physiopathology*; D044622:Dental Pellicle; D004357:Drug Synergism; D006801:Humans; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D066298:In Vitro Techniques; D007501:Iron / Q000009:adverse effects* / Q000032:analysis; D012463:Saliva / Q000502:physiology; D013634:Tannins / Q000494:pharmacology*; D013997:Time Factors; D014002:Tin Fluorides / Q000627:therapeutic use; D014075:Tooth Discoloration / Q000139:chemically induced* / Q000517:prevention & control
D047348:Hydrolyzable Tannins; D013634:Tannins; D014002:Tin Fluorides; D007501:Iron; D002710:Chlorhexidine