Radioimmunoassay compared to an enzymatic method for serum bile acid determination.
Radioimmunoassay (RIA) of cholic and chenodeoxycholic acid was compared to a total bile acid determination with 3 alpha-hydroxysteroid dehydrogenase (3 alpha-HSD) and a gas liquid chromatographic (GLC) determination of individual bile acids. When sera from patients with increased bile acid concentration were analysed the results indicated a good correlation between GLC and the other methods. Analysis of sera from healthy subjects indicated a good correlation between GLC and RIA. There exists no correlation between RIA and 3 alpha-HSD when serum bile acids were analysed in healthy subjects in part due to the presence of varying amounts of secondary bile acids.
D015096:3-Hydroxysteroid Dehydrogenases; D001647:Bile Acids and Salts / Q000097:blood*; D002635:Chenodeoxycholic Acid / Q000097:blood*; D002793:Cholic Acids / Q000097:blood*; D002849:Chromatography, Gas; D006801:Humans; D011863:Radioimmunoassay
D001647:Bile Acids and Salts; D002793:Cholic Acids; D002635:Chenodeoxycholic Acid; D015096:3-Hydroxysteroid Dehydrogenases