title: [Genetic instability of the production of antibiotic substances controlled by the SCP2 plasmid of Streptomyces coelicolor A3(2)].
abstract: Streptomyces coelicolor A3(2) strain bearing a variant of SCP2 plasmid produces three antibiotic substances: amromycin and streptocins A and B. After growth at elevated temperature (37 degrees C) of S. coelicolor A3(2), mutants which are not capable of producing amromycin and streptocins A and B (ant- mutants) were formed at high frequency (50%). These mutants retained SCP2 plasmid. Genetic analysis demonstrates that Ant phenotype is caused by a mutation in SCP2 plasmid. Genetic analysis demonstrates that Ant phenotype is caused by a mutation in SCP2. Heteroduplex and electrophoretic analysis of SCP2 DNAs from mutant variants indicates that these plasmids contained the identical deletion of 800 +/- 100 base pairs, as compared to SCP2 DNA of the initial strain. Ant variants revert to the initial phenotype at a frequency of 1.10(-2) to 1.10(-3). In turn, revertants from Ant variants a high frequency, similar to the initial strain. It is suggested that reversible Ant+ in equilibrium or formed from Ant- transitions might be the result of transposition of a genetic element in SCP2 controlling production of amromycin and streptocins A and B.
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001430:Bacteriocins / Q000096:biosynthesis*; D004269:DNA, Bacterial / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D011995:Recombination, Genetic; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism; D048372:Streptomyces coelicolor; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D004269:DNA, Bacterial; D005819:Genetic Markers; C009325:streptocin
vernacular_title: Geneticheskaia nestabil'nost' produktsii antibioticheskikh veshchestv, kontroliruemoĭ SCP2-plazmidoĭ Streptomyces coelicolor A3(2).