Idiotypic relationship of anti-Ia.2 antibodies.
Alloantiserum and hybridoma-derived anti-Ia antibodies were investigated for their idiotypic similarity using 4 monoclonal anti-idiotypes raised against monoclonal anti-Ia. With one anti-idiotype, idiotypic cross-reactivity could be found between three independently derived monoclonal BALB/c anti-Ia antibodies, all of which were directed against the same serological determinant, Ia.2. The respective idiotype, tentatively designated Ia.2 idiotope, could also be demonstrated on anti-Ia.2 antibodies in BALB/C alloantisera, but not on anti-Ia against other specificities. The expression of the Ia.2 idiotope is linked to the Igh allotype; the use of Ig-recombinant mice allowed us to map the Igh-Ia.2 locus to the left of the T15 marker in the Igha V region.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D003429:Cross Reactions; D008040:Genetic Linkage; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D007106:Immune Sera / Q000235:genetics / Q000276:immunology; D007126:Immunoglobulin Allotypes / Q000235:genetics; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D007518:Isoantibodies / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D007106:Immune Sera; D007126:Immunoglobulin Allotypes; D007130:Immunoglobulin Idiotypes; D007518:Isoantibodies