In vitro sensitivity of nitrosourea-induced neurogenic tumors to ACNU.
The effect of ACNU on the in vitro viability of a methylnitrosourea-induced gliosarcoma (T9) and two ethylnitrosourea-induced brain tumors, TR-481 (a malignant neurinoma) and EB-679 (a glioma) was studied. T9 was highly sensitive to ACNU, demonstrating loss of cells following a 3 hour exposure time to 5 microgram/ml; TR-481 was sensitive to 40 microgram/ml of ACNU and EB-679 was highly resistant to 40 microgram/ml of ACNU. The in vitro sensitivity of the tumor cell lines to ACNU is: T 9 greater than TR-481 greater than EB-679. This data indicates that variability of response to both concentration and exposure time of ACNU of malignant brain tumor cells must be taken into consideration in planning in vitro and/or in vivo treatment of experimental brain tumors.
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D001932:Brain Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000648:ultrastructure; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005038:Ethylnitrosourea; D005910:Glioma / Q000139:chemically induced / Q000188:drug therapy* / Q000648:ultrastructure; D066298:In Vitro Techniques; D008770:Methylnitrosourea; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000648:ultrastructure; D009442:Neurilemmoma / Q000139:chemically induced / Q000188:drug therapy* / Q000648:ultrastructure; D015376:Nimustine; D009607:Nitrosourea Compounds / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D011916:Rats, Inbred F344; D013997:Time Factors
D000970:Antineoplastic Agents; D009607:Nitrosourea Compounds; D015376:Nimustine; D008770:Methylnitrosourea; D005038:Ethylnitrosourea