title: Use of plasmid R68.45 for constructing a circular linkage map of the Rhizobium trifolii chromosome.
abstract: Plasmid R68.45 was used to promote conjugal transfer of chromosomal markers in Rhizobium trifolii RS55. Analysis of two-factor and three-factor crosses among R. trifolii strains enabled construction of a circular linkage map of the R. trifolii chromosome, containing 17 nutritional and resistance markers.
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D005798:Genes, Bacterial; D008040:Genetic Linkage; D005819:Genetic Markers; D011815:R Factors; D012231:Rhizobium / Q000235:genetics*
chemical_list: D005819:Genetic Markers