Mouse beta-globin and adenovirus-2 major late transcripts are initiated at the cap site in vitro.
Initiation of transcription on major late DNA of adenovirus serotype 2 and the beta-globin gene of the mouse was studied by using ribonucleoside [beta-32P]triphosphates as labeled precursors in vitro. The 5' termini of discrete runoff transcripts of both templates were preferentially labeled. The beta phosphate of the cap structure was labeled by [beta-32P]ATP but not [beta-32P]GTP, indicating that the beta phosphate of the cap is contributed by the initiating nucleoside triphosphate (ATP in both cases). Therefore, capping occurs on the initial nucleotide of these transcripts.
D000260:Adenoviruses, Human / Q000235:genetics*; D001483:Base Sequence; D002474:Cell-Free System; D005914:Globins / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D012315:RNA Caps / Q000378:metabolism*; D014158:Transcription, Genetic
D012315:RNA Caps; D005914:Globins