The tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate inhibits or enhances induction of differentiation of mouse myeloid leukemia cells depending on the type of serum in the medium.
The tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) inhibited the induction of both functional and morphological differentiation of mouse myeloid leukemia cells (Ml) cultured in medium containing 10% calf serum, but enhanced these inductions in medium containing 10% fetal calf serum and several inducers. These results suggest that some factor(s) in sera modifies the differentiation-inducing action of TPA on leukemia Ml cells.
D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media; D005312:Fetal Blood; D007942:Leukemia, Experimental / Q000473:pathology; D007951:Leukemia, Myeloid / Q000473:pathology*; D051379:Mice; D010586:Phagocytes / Q000187:drug effects; D010704:Phorbols / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D003470:Culture Media; D010704:Phorbols; D013755:Tetradecanoylphorbol Acetate