Endothelial surface charge: blood-brain barrier opening to horseradish peroxidase induced by the polycation protamin sulfate.
Perfusion of rat brain with the polycation protamin sulfate caused a decrease in the stainability of luminal surface of cerebral endothelium to positively charged colloidal iron. This change reflecting a decrease in the anionic sites of the endothelial plasma membrane was accompanied by opening of the blood-brain barrier to horseradish peroxidase. The polyanion heparin reversed colloidal iron binding but failed to prevent permeability alteration of the cerebral endothelium.
D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects*; D002199:Capillary Permeability / Q000187:drug effects; D002352:Carrier Proteins / Q000378:metabolism; D004727:Endothelium / Q000187:drug effects; D006735:Horseradish Peroxidase / Q000378:metabolism*; D007501:Iron / Q000378:metabolism; D033862:Iron-Binding Proteins; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008854:Microscopy, Electron; D010296:Parietal Lobe / Q000098:blood supply; D010544:Peroxidases / Q000378:metabolism*; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers / Q000494:pharmacology*; D051381:Rats; D033863:Transferrin-Binding Proteins
D002352:Carrier Proteins; D006735:Horseradish Peroxidase; D007501:Iron; D033862:Iron-Binding Proteins; D010544:Peroxidases; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers; D033863:Transferrin-Binding Proteins