The effect of human dental plaque on bone resorption and hyaluronic acid synthesis in in-vitro cultures of fetal rat calvaria.
Dental bacterial plaque affects bone demineralization both in vivo and in vitro. Since hyaluronic acid may play a role in demineralization, calcium release into the media as a measure of bone resorption was correlated with the synthesis of [3H]-glucosamine-labelled macromolecules in bone culture. The synthesis of glycosaminoglycans was determined by both a Millipore method and by DEAE-cellulose chromatography. Addition of plaque extract to the culture media stimulated both the synthesis and release of hyaluronic acid to the culture media, while stimulating calcium release. Thus, the bone-demineralizing effect of dental plaque seems to be associated to the induced synthesis of hyaluronic acid.
D000818:Animals; D001862:Bone Resorption / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002848:Chromatography, DEAE-Cellulose; D046508:Culture Techniques; D003773:Dental Plaque / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D051381:Rats; D012886:Skull / Q000196:embryology
D006025:Glycosaminoglycans; D006820:Hyaluronic Acid; D002118:Calcium