Filterability and bank blood conservation media.
A study was made of storage lesions of packed red cells stored in different preservative media: CPD, CPD-adenine, CPD-SAG, and CPD-pentoxifylline. The following chemical and physical parameters were studied: filterability, viscosity, ATP, 2-3,DPG pH, osmotic fragility, scanning electron microscopy pattern, blood gas levels. Tests were performed on days 0, 2, 4, 8, 16 and 24. The number and intensity of storage lesions in vitro give an indication of changes in oxygen-delivery capacity and flow characteristics of erythrocytes over a period of storage at 4 degrees C in different preservative media. CPD was found to maintain pertinent RBC properties up to Day 15. Adding adenine made little difference, whereas adding SAG gave better results. Pentoxifylline did not show appreciable effects in the initial concentrations used, which were close to those employed in medical practice. A preservative mixture ensuring maintenance of normal blood values during storage at 4 degrees C has yet to be found. Continued study of pentoxifylline should be conducted using higher concentrations of this additive.
D000328:Adult; D001771:Blood Banks; D001793:Blood Preservation; D002951:Citrates / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000502:physiology*; D004912:Erythrocytes / Q000502:physiology*; D005260:Female; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D008297:Male; D008851:Micropore Filters; D010431:Pentoxifylline / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism
D002951:Citrates; C007077:citrate phosphate dextrose; D012964:Sodium; D005947:Glucose; D011188:Potassium; D010431:Pentoxifylline