Anthracycline assay by high-pressure liquid chromatography.
A general method of analysis of anthracycline concentrations was developed. Drug is extracted from plasma with organic solvent and separated from metabolites by high-pressure liquid chromatography on an aminocyanosilica column. Detection and quantitation are by the endogenous fluorescence of compounds having an intact tetracyclic ring structure. Limits of sensitivity are 5, 1, and 5 ng/ml of plasma for doxorubicin, carubicin, and marcellomycin, respectively. The assay can be used for studying the aldo-keto reductase and reductive glycosidase reactions with anthracyclines as the substrates and for the evaluation of the clinical pharmacology or pharmacodynamics of various doxorubicin analogs.
D000900:Anti-Bacterial Agents / Q000097:blood*; D000903:Antibiotics, Antineoplastic; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003630:Daunorubicin / Q000097:blood*; D004317:Doxorubicin / Q000097:blood*; D006027:Glycosides / Q000097:blood; D006801:Humans; D007700:Kinetics; D009279:Naphthacenes / Q000097:blood*
D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D006027:Glycosides; D009279:Naphthacenes; D004317:Doxorubicin; D003630:Daunorubicin