Sulfation of minoxidil by liver sulfotransferase.
The 100,000 g supernatant fraction of rat liver homogenate contains a sulfotransferase activity which catalyzes the sulfation of minoxidil. Synthetic minoxidil N-O sulfate and the enzyme synthesized product had identical chromatographic characteristics on high pressure liquid chromatography. Minoxidil sulfate, which yields minoxidil when treated with sulfatase, was slowly hydrolyzed in water. Several N-oxides of other heterocycles, including several other pyrimidines, triazines and imidazoles, were also substrates for this sulfotransferase.
D000818:Animals; D001711:Biotransformation; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D008297:Male; D008914:Minoxidil / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013466:Sulfurtransferases / Q000378:metabolism*
D011743:Pyrimidines; D008914:Minoxidil; D013466:Sulfurtransferases