Comparison of in situ and peripheral host immunity to syngeneic tumours employing the multicellular spheroid model.
The multicellular tumour spheroid was used as a model system to assess the in situ host immune response to the EMT6/Ro mammary tumour in syngeneic BALB/cKa mice. In sensitized mice the spheroids were rapidly infiltrated by host cells including macrophages, lymphocytes and granulocytes. Tumour cell killing was evident within 1 day and resulted in the eventual complete destruction of the spheroids. Host cells within the spheroids had a greater cytolytic capacity than the surrounding peritoneal cells and virtually no cytolytic activity was detectable in cells from the spleen. A similar discrepancy between in situ and peripheral immunity was found in mice bearing solid EMT6/Ro tumours.
D000818:Animals; D000918:Antibody Specificity; D002465:Cell Movement; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008954:Models, Biological; D009368:Neoplasm Transplantation; D013154:Spleen / Q000276:immunology; D013997:Time Factors; D014185:Transplantation, Isogeneic