Molecular cloning of a human histocompatibility antigen cDNA fragment.
A clone (pHLA-1) containing HLA-specific cDNA was constructed by reverse transcription of partially purified HLA mRNA from the human lymphoblastoid cell line LKT. The identity of pHLA-1 was established by its ability to hybridize to HLA heavy chain mRNA and by nucleotide sequence analysis. The pHLA-1 cDNA insert (approximately 525 base pairs) corresponds to the COOH-terminal 46 amino acids of an HLA-A, -B, or -C antigen (15 residues from the hydrophobic region and the remainder from the COOH-termial hydrophilic region), together with a portion of the 3' untranslated region of the mRNA.
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D003062:Codon; D004274:DNA, Recombinant; D005796:Genes; D006680:HLA Antigens / Q000235:genetics*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics
D003062:Codon; D004274:DNA, Recombinant; D006680:HLA Antigens; D012333:RNA, Messenger