The effect of extracts from human dental plaque, oral streptococci and actinomyces bacteria on HELA cell growth, [3H]-thymidine uptake and 51Cr release.
Dialysed extracts from dental plaque and a number of species of oral streptococci and actinomycetes have been investigated for their effects on cell growth, [3H]-thymidine uptake and 51Cr release from HeLa cell cultures. Eight of the 15 extracts inhibited cell growth and all but one inhibited [3H]-thymidine incorporation. None of the extracts stimulated 51Cr release from prelabelled cells. These results suggest that many of the species of streptococci and actinomyces found in the mouth and in dental plaque are capable of producing high mol. wt substances that interfere with HeLa cell cultures by inhibiting [3H]-thymidine uptake and DNA synthesis but do not affect the degree of intactness of the external cell membrane.
D000190:Actinomyces / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002860:Chromium Radioisotopes / Q000378:metabolism; D003773:Dental Plaque / Q000378:metabolism*; D006367:HeLa Cells / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D009055:Mouth / Q000382:microbiology; D013291:Streptococcus / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014020:Tissue Extracts / Q000494:pharmacology
D002860:Chromium Radioisotopes; D014020:Tissue Extracts; D013936:Thymidine