[Role of the nucleus and the cytoplasm in determining the change in the permeability for colchichine of the plasma membrane of mouse cells].
A mouse cell line, B-82CH/CAP, with three genetic markers - resistance to 5-bromodeoxyuridine (5-BUdR), due to the deficiency in thymidine kinase (TK); resistance to colchicine, due to a decrease in membrane permeability to the drug; and resistance to chloramphenicol (CAP), was obtained. The capacity of cytoplasts and karyoplasts prepared from this cell line, to transfer resistance to colchicine and CAP was studied. Cytoplasts prepared from B-82CH/CAP cells were fused to karyoplasts prepared either from the mouse L or hamster DM-15 cell line, both of which are sensitive to colchicine and to CAP. The hybrid cells were resistant to CAP, but sensitive to colchicine. However, when karyoplasts prepared from B-82CH/CAP cells were fused to cytoplasts prepared from L cells, the hybrids retained the capacity to proliferate in the medium containing colchicine and 5-BUdR, but were greatly reduced in their ability to grow in the medium containing CAP. These results indicate that the resistance of B-82CH/CAP cells to CAP can be cytoplasmically transmitted and, as in other CAP-resistant cell lines, is probably due to a mutation in mitochondrial DNA. Changes in membrane permeability associated with colchicine-resistance appear to be determined by the nucleus.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002467:Cell Nucleus / Q000187:drug effects* / Q000648:ultrastructure; D002470:Cell Survival / Q000187:drug effects; D002701:Chloramphenicol / Q000494:pharmacology; D003078:Colchicine / Q000494:pharmacology*; D006224:Cricetinae; D003593:Cytoplasm / Q000187:drug effects* / Q000648:ultrastructure; D004351:Drug Resistance; D005819:Genetic Markers; D006822:Hybrid Cells / Q000187:drug effects / Q000648:ultrastructure; D007739:L Cells / Q000187:drug effects / Q000648:ultrastructure; D051379:Mice
D005819:Genetic Markers; D002701:Chloramphenicol; D001973:Bromodeoxyuridine; D003078:Colchicine
Rol' iadra i tsitoplazmy v determinatsii izmeneniia pronitsaemosti dlia kolkhitsina plazmaticheskoĭ membrany kletok myshi.