Sensitivity to 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2-chloroethyl)-3-nitrosourea hydrochloride (ACNU) of glioma cells in vivo and in vitro.
A new water-soluble nitrosourea (ACNU) was tested for its antitumor activity against four glioma cell lines. Four factors were studied to determine its antitumor activity: inhibition of cell growth, morphologic observation, analysis of DNA histogram with flow microfluorometry, and sensitivity testing with microtest plate. Growth inhibition in response to ACNU was seen in two cell lines (EA285, U251-MG), whereas two cell lines (YE2-02, T98) were resistant to ACNU. The results of the present sensitivity test concur with those of other examinations that this test is useful in selecting a drug and determining the effective dose.
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001932:Brain Neoplasms / Q000188:drug therapy; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004353:Drug Evaluation, Preclinical; D005910:Glioma / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D007700:Kinetics; D009374:Neoplasms, Experimental / Q000188:drug therapy / Q000473:pathology; D015376:Nimustine; D009607:Nitrosourea Compounds / Q000627:therapeutic use*; D051381:Rats
D000970:Antineoplastic Agents; D009607:Nitrosourea Compounds; D015376:Nimustine