title: Chondrocytes contain a growth factor that is localized in the nucleus and is associated with chromatin.
abstract: Bovine scapular and articular chondrocytes were isolated from fresh cartilage and disrupted by sonication. The disrupted cells had the ability to stimulate DNA synthesis and cell division in vitro in chondrocytes and in 3T3 cells. Subcellular fractions were prepared by two methods, enucleation with cytochalasin B and lysis of cells with Nonidet P-40. After enucleation of chondrocytes, karyoplasts and cytoplasts were collected, disrupted by sonication, and tested for their ability to stimulate DNA synthesis. Over 95% of the cellular growth factor activity was localized in the karyoplast. In addition, after lysis of chondrocytes in Nonidet P-40, over 95% of the growth factor activity was recovered in the nuclear fraction. Chondrocyte chromatin was prepared by low ionic strength detergent treatment of karyoplasts. All of the growth factor activity of the karyoplast was found to be associated with chromatin. The growth factor activity of chondrocytes, cytoplasts, karyoplasts, and chromatin was analyzed by gel filtration on Bio-Gel A-0.5 m equilibrated with 4 M guanidine . HCl and 5 mM dithiothreitol. Chondrocytes, chondrocyte karyoplasts, and chondrocyte chromatin had similar column elution profiles, with molecular weights in the range of 12,000-22,000.
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology*; D002358:Cartilage, Articular / Q000166:cytology; D002417:Cattle; D002455:Cell Division; D002467:Cell Nucleus / Q000502:physiology; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis; D006133:Growth Substances / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D002843:Chromatin; D006133:Growth Substances; D004247:DNA