Studies with [35S]methionine indicate that the 22,000-dalton [Met]enkephalin-containing protein in chromaffin cells is a precursor of [Met]enkephalin.
It has been shown that [35S]methionine is incorporated into the [Met]enkephalin sequences of a 22,000-dalton enkephalin-containing protein in the adrenal medulla. Pulse-chase experiments indicate that label is incorporated into the large polypeptide before it appears in free [Met]enkephalin and a smaller [Met]enkephalin-containing peptide. These findings provide direct evidence of a precursor-product relationship of these structurally related polypeptides.
D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002838:Chromaffin System / Q000378:metabolism; D004723:Endorphins / Q000096:biosynthesis*; D004745:Enkephalins / Q000096:biosynthesis*; D008970:Molecular Weight; D011498:Protein Precursors / Q000378:metabolism*
D004723:Endorphins; D004745:Enkephalins; D011498:Protein Precursors