Prostaglandin production and cellular aging.
Prostaglandin (PG) production by human embryo lung fibroblasts (HELF) is stimulated by a number of effectors including angiotensin, thrombin, bradykinin and ascorbic acid. The types of prostaglandins produced are to a degree effector-dependent. For example, angiotensin stimulates mainly PGE2 synthesis, thrombin stimulates production of both PGE2 and prostacyclin while bradykinin and ascorbic acid stimulate production of PGE2, PGF2 alpha, prostacyclin and thromboxane A2. Upon senescence in culture, prostacyclin (PGI2) production by HELF drops dramatically in response to ascorbic or arachidonic acids. An overall drop in prostaglandin synthesis is observed with bradykinin stimulation. Prostaglandin production is also related to senescence in human skin fibroblasts (HSF). These cells produce predominantly PGI2. Prostacyclin production in response to bradykinin drops in HSF as they are obtained from individuals of increasing chronologic age. Thus our results indicate changes in prostaglandin production upon senescence, a dependency of these changes on the given stimulus and a correlation between in vivo and in culture aging with respect to prostaglandin production.
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000328:Adult; D000368:Aged; D000375:Aging; D000804:Angiotensin II / Q000494:pharmacology; D002478:Cells, Cultured; D015237:Dinoprost; D015232:Dinoprostone; D004837:Epinephrine / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D008168:Lung; D008297:Male; D008875:Middle Aged; D011453:Prostaglandins / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis; D011460:Prostaglandins F / Q000096:biosynthesis; D013917:Thrombin / Q000494:pharmacology; D013929:Thromboxane B2 / Q000096:biosynthesis
D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D000804:Angiotensin II; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost; D013917:Thrombin; D015232:Dinoprostone; D004837:Epinephrine