In vitro-constructed RP4-prime plasmids mediate orientated mobilization of the Proteus morganii chromosome.
RP4-prime plasmids containing inserts of Proteus morganii strain 2815 chromosomal DNA (0.5-13 megadaltons in size) were constructed in vitro. When introduced into the latter organism, the hybrid plasmids promoted transfer of chromosomal markers in an orientated manner, with recombinant frequencies from 10(-4) to 10(-6) per donor. Transconjugants expressed the tetracycline-resistant phenotype of the plasmids. Plasmid-guided trajectories overlapped and a circular map of chromosome markers was constructed. Correspondence between size of inserted chromosomal DNA in hybrid plasmids and numbers of markers transferred suggested that homology played a role in chromosome mobilization by these plasmids.
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D005819:Genetic Markers; D010957:Plasmids; D011511:Proteus / Q000235:genetics*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D005819:Genetic Markers