title: In vivo and in vitro studies of inhibitory effects of restorative dental materials on salivary esterases.
abstract: The influence of 18 dental materials on the activity of esterases isolated from human whole saliva has been studied. Eugenol on its own was a strong competitive inhibitor of the esterases II-2a (Ki = 3.3/10(-5) M) and II-2b (Ki = 4.7/10(-6) M). Except for Luralite all the materials that contained eugenol as one of the components (IRM, Nobetec and Opotow) were noncompetitive inhibitors. Additional in vitro inhibitors were: Adaptic, Aspa, Coe-pack and the amalgam ANA 68. Treatment with Nobetec packs in vivo resulted in a decrease activity of whole saliva.
mesh_terms: D003764:Dental Materials / Q000494:pharmacology*; D004950:Esterases / Q000302:isolation & purification / Q000378:metabolism*; D005054:Eugenol / Q000494:pharmacology; D005260:Female; D006801:Humans; D008297:Male; D012463:Saliva / Q000201:enzymology*; D013053:Spectrophotometry; D013997:Time Factors
chemical_list: D003764:Dental Materials; D005054:Eugenol; D004950:Esterases