High-frequency cotransfer of the transformed phenotype and a tumor-specific transplantation antigen by DNA from the 3-methylcholanthrene-induced Meth A sarcoma of BALB/c mice.
We transformed BALB/3T3 mouse cells with cellular DNA extracted from the Meth A sarcoma, a 3-methylcholanthrene-induced tumor of BALB/c mice, and asked whether foci arising in the transfection possess the previously defined Meth A tumor-specific transplantation antigen (TSTA). Five of eight foci selected from one experiment possessed Meth A TSTA. DNA extracted from one of the five TSTA-positive clones was used in secondary rounds of transfection transformation. Four out of five foci tested from the secondary transfections possessed Meth A TSTA. These results suggest that in the Meth A sarcoma a transforming gene and a genetic determinant of TSTA are intimately related: they may be identical or very closely linked; alternatively, a particular transforming gene might induce the expression of a particular TSTA. Another possible explanation for these results is that the cotransfer of certain cellular genes by DNA transfection is considerably higher than predicted from the limited studies presently available.
D000818:Animals; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm / Q000235:genetics*; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000235:genetics*; D008040:Genetic Linkage; D051379:Mice; D012513:Sarcoma, Experimental / Q000235:genetics* / Q000276:immunology; D014170:Transformation, Genetic
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm