Enzyme induction in Streptomyces hydrogenans. Comparison of the effects of different steroids to increase the activity of 3 alpha, 20 beta-hydroxysteroid dehydrogenase and of 3 beta, 17 beta-hydroxysteroid dehydrogenase.
After cultivation of Streptomyces hydrogenans in the presence of different steroids the activity of both 3 alpha, 20 beta-hydroxysteroid dehydrogenase and 3 beta, 17 beta-hydroxysteroid dehydrogenase was determined in the cell homogenate of the microorganism. By comparing the efficacy of the steroids to increase enzyme activities, steroids could be divided into 3 groups: a) steroids which stimulated preferentially the activity of 3 alpha, 20 beta-hydroxysteroid dehydrogenase (e. g., corticosterone), b) steroids which stimulated preferentially 3 beta, 17 beta-hydroxysteroid dehydrogenase (estradiol-17 beta), and c) those behaving intermediately (e. g., progesterone, 5 alpha -dihydrotestosterone). Highest 3 beta, 17 beta-hydroxysteroid dehydrogenase activity could be measured 2 h after addition of 5 alpha-dihydrotestosterone to the culture medium. The activity of 3 alpha, 20 beta-hydroxysteroid dehydrogenase, however, increased continuously up to 4 h. 3 alpha, 20 beta-hydroxysteroid dehydrogenase and 3 beta, 17 beta-hydroxysteroid dehydrogenase syntheses seemed to be controlled by steroids in a non-coordinate manner.
D015067:17-Hydroxysteroid Dehydrogenases / Q000096:biosynthesis*; D015089:20-Hydroxysteroid Dehydrogenases / Q000096:biosynthesis*; D003345:Corticosterone / Q000494:pharmacology; D013196:Dihydrotestosterone / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects*; D004958:Estradiol / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology; D011374:Progesterone / Q000494:pharmacology; D013256:Steroids / Q000494:pharmacology*; D013302:Streptomyces / Q000187:drug effects* / Q000201:enzymology; D013739:Testosterone / Q000494:pharmacology
D013256:Steroids; D013196:Dihydrotestosterone; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D015067:17-Hydroxysteroid Dehydrogenases; D015089:20-Hydroxysteroid Dehydrogenases; D003345:Corticosterone; D006854:Hydrocortisone