title: Structure of Ia antigens: identification of dimeric complexes formed by the invariant chain.
abstract: A 2-dimensional gel technique was used for the identification of disulfide-linked polypeptide complexes formed by Ia chains and their corresponding subunits. Several dimers containing the invariant (Ii) chain were found. The most prominent one consisted of 2 Ii chains. In addition, Ii molecules were found to be covalently linked to 3 unknown components of 41,000, 27,000, and 10,000 to 15,000 m.w. The dimeric form of Ii exists also after alkylation of free SH-groups with iodoacetamide, which suggests that formation of the Ii dimer is not the result of experimental conditions. Furthermore, noncovalently associated dimers formed by processed alpha- and beta-chains as well as by their precursors alpha p and beta p could be detected. No mixed associated chains composed of precursors and processor molecules were observed, which indicates that processing and insertion into the membrane take place only after dimerization.
mesh_terms: D000478:Alkylation; D000818:Animals; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D007460:Iodoacetamide / Q000494:pharmacology; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D011487:Protein Conformation; D013154:Spleen / Q000032:analysis
chemical_list: D000949:Histocompatibility Antigens Class II; D046911:Macromolecular Substances; D007460:Iodoacetamide