title: Nickel-containing factor F430: chromophore of the methylreductase of Methanobacterium.
abstract: The yellow chromophore of the methyl-coenzyme M methylreductase of Methanobacterium thermoautotrophicum has been found to be the nickel-containing factor F430. Treatment of 63Ni-labeled methylreductase with 80% aqueous methanol released the radiolabel as well as the yellow chromophore; both properties were associated with a single compound that was found to be identical to F430, the stoichiometry being 1 mol of nickel per mol of F430 and 2 mol of F430 per mol of methylreductase.
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D019605:Euryarchaeota / Q000201:enzymology*; D008665:Metalloporphyrins; D008667:Metalloproteins / Q000378:metabolism*; D009532:Nickel / Q000378:metabolism* / Q000502:physiology*; D010088:Oxidoreductases / Q000378:metabolism*
chemical_list: D008665:Metalloporphyrins; D008667:Metalloproteins; C023540:factor F430; D009532:Nickel; D010088:Oxidoreductases; C023895:methyl coenzyme M methylreductase