Glycogen storage disease type X caused by ochratoxin A in broiler chickens.
Day-old broiler chickens fed graded concentrations of ochratoxin A (0, .5, 1.0, 2.0, 4.0, and 8.0 micrograms/g of diet) for 3 weeks were examined histopathologically for glycogen accumulation in muscle tissue using both formalin and ethanol fixatives with periodic acid-Schiff stain in a duplicate diastatic slide technique. However, glycogen could not be visualized in chicken muscle by these techniques which work with human muscle. Quantitative analysis for the glycogen itself permitted demonstration of a significant (P less than .05) fourfold increase in birds fed 8 micrograms/g. Using startled birds demonstrated that this accumulated glycogen was about 99% physiologically available. These findings coupled with prior reports permit the assignment on morphologic criteria of the hyperglycogenation of ochratoxicosis as a type X glycogen storage disease.
D000818:Animals; D002645:Chickens; D006008:Glycogen Storage Disease / Q000139:chemically induced / Q000378:metabolism / Q000662:veterinary*; D008297:Male; D009132:Muscles / Q000032:analysis; D009793:Ochratoxins / Q000009:adverse effects*; D011201:Poultry Diseases / Q000139:chemically induced* / Q000378:metabolism
D009793:Ochratoxins