Ultrastructural alteration of trypsin- and pancreatin-separated embryonic rabbit palate epithelium and mesenchyme.
The effects of 30- and 45-minute trypsin- and pancreatin-separation on embryonic rabbit palate epithelium and mesenchyme were studied with the electron microscope. Changes in epithelium included fragmentation of the basal lamina and formation of cytoplasmic blebs associated with the basal cell layer. Changes in mesenchymal cells were first evident at 45 min of incubation and included cell fragmentation, nuclear pyknosis, and dilation of extracellular space. Results indicate a differential susceptibility of mesenchyme to the separating agent.
D000818:Animals; D046508:Culture Techniques; D004848:Epithelium / Q000187:drug effects / Q000648:ultrastructure; D008648:Mesoderm / Q000187:drug effects / Q000648:ultrastructure*; D010159:Palate / Q000187:drug effects / Q000196:embryology* / Q000648:ultrastructure; D010194:Pancreatin / Q000494:pharmacology*; D011817:Rabbits; D014357:Trypsin / Q000494:pharmacology*
D010194:Pancreatin; D014357:Trypsin