DNA sequence associated with chromosome translocations in mouse plasmacytomas.
A DNA sequence that generates aberrantly rearranged immunoglobulin heavy chain constant region genes in murine plasmacytomas is shown to participate in a chromosome translocation. We have previously termed this DNA sequence NIARD for non-immunoglobulin-associated rearranging DNA. NIARD rearrangements were found frequently in murine plasmacytomas but were not detected in normal lymphocytes. These rearrangements occasionally involve the switch region of the C alpha gene. In this study, DNA samples obtained from mouse-Chinese hamster somatic cell hybrid lines were digested with various restriction endonucleases and analyzed by the Southern transfer technique with a NIARD hybridization probe. These experiments show that NIARD resides on chromosome 15 in the mouse germ line. Since NIARD is found adjacent to the C alpha gene (located on chromosome 12) in some plasmacytomas, it is apparent that a translocation involving these two chromosomes has occurred. We have proposed a rcpT(12;15) model to explain our data. The implications of NIARD rearrangements for malignant transformation are discussed.
D000818:Animals; D002874:Chromosome Mapping; D005786:Gene Expression Regulation; D005796:Genes; D007136:Immunoglobulins / Q000235:genetics*; D051379:Mice; D009374:Neoplasms, Experimental / Q000235:genetics; D010954:Plasmacytoma / Q000235:genetics*; D014178:Translocation, Genetic
D007136:Immunoglobulins