Affinity chromatography of terminal deoxynucleotidyl tranferase from calf thymus and human leukemic cells on a solid-phase immunoadsorbent.
A solid-phase immunoadsorbent specific for terminal deoxynucleotidyl transferase has been prepared. The enzyme from calf thymus and acute lymphoblastic leukemia cells binds to columns of this material. Bound enzyme can be eluted in an active form. Selective and rapid purification of terminal deoxynucleotidyl transferase from crude extracts of cells containing this enzyme can be achieved by this method since the immunoadsorbent has no affinity for other cellular DNA polymerases.
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D004254:DNA Nucleotidyltransferases / Q000302:isolation & purification*; D006801:Humans; D007106:Immune Sera; D007074:Immunoglobulin G; D007945:Leukemia, Lymphoid / Q000201:enzymology*; D011817:Rabbits / Q000276:immunology; D013950:Thymus Gland / Q000201:enzymology*
D007106:Immune Sera; D007074:Immunoglobulin G; D004254:DNA Nucleotidyltransferases