Solubilization of 17 beta-hydroxysteroid dehydrogenase from rat liver microsomes.
The conditions for the solubilization of 17 beta-hydroxysteroid dehydrogenase from a rat liver microsomal preparation with the non-ionic detergent Triton X-100 were studied. The recoveries of 17 beta-hydroxysteroid dehydrogenase activity and of proteins in the solubilized form were determined as a function of detergent concentration, of pH and temperature, of incubation time and of saline concentration. The soluble fraction obtained under the optimal conditions contained 80% of the proteins and 75% of the enzymatic activity of initial microsomes. The presence of Triton X-100 in the solubilized proteins was not essential for enzyme activity.
D015067:17-Hydroxysteroid Dehydrogenases / Q000302:isolation & purification*; D000818:Animals; D005260:Female; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008862:Microsomes, Liver / Q000201:enzymology*; D009994:Osmolar Concentration; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride / Q000494:pharmacology; D012995:Solubility; D013696:Temperature
D012965:Sodium Chloride; D015067:17-Hydroxysteroid Dehydrogenases