[Nuclease activity of blood serum from healthy persons and from patients with some hematologic impairments].
Nuclease activity was studied using as a substrate native RNA as well as synthetic polyribonucleotides polyU and polyU-polyA in blood serum of healthy persons and of patients with leukoses and myelofibrosis. In the hematologic impairments no distinct correlation was observed between the nuclease activity with either native RNA or the polyribonucleotides as substrates. The RNAase activity was markedly increased only under conditions of chronic myeloleukosis, whereas a significant increase in the nuclease activity using polyU was found in 42.8% of the patients with acute leukosis, in 61.5% of the patients with chronic myeloleukosis, in 68,7% of the patients with chronic lympholeukosis and in 77.8% of the patients with myelofibrosis; the elevation in the nuclease activity with polyU-polyA as a substrate observed in 28,5%, 15,4%, 12,5% and 33.3% of the patients, respectively.
D006801:Humans; D007700:Kinetics; D007938:Leukemia / Q000201:enzymology*; D007945:Leukemia, Lymphoid / Q000201:enzymology; D007951:Leukemia, Myeloid / Q000201:enzymology; D055728:Primary Myelofibrosis / Q000201:enzymology; D012016:Reference Values; D012260:Ribonucleases / Q000097:blood*; D013379:Substrate Specificity
D012260:Ribonucleases
Nukleaznaia aktivnost' syvorotki krovi zdorovykh liudeĭ i bol'nykh s nekotorymi gematologicheskimi zabolevaniiami.