Noncytolytic extraction of murine tumor-specific transplantation antigens with the nonionic detergent octyl-beta-D-glucopyranoside.
The nonionic detergent octyl-beta-D-glucopyranoside (C8Glu) was used to extract immunogenic tumor-specific transplantation antigen (TSTA) from intact cells and purified plasma membranes of the 3-methylcholanthrene-induced fibrosarcoma MCA-F. Pretreatment of syngeneic C3H/HeJ mice with 100 micrograms of C8Glu extracts induced specific immunoprotection such that mice resisted the outgrowth of MCA-F but not the antigenically distinct tumors MCA-D or MCA-2A. Incubation of intact cells with 7 mM (0.2%) C8Glu for 30 minutes at 23 degrees C was judged to be noncytolytic because extracted cells excluded trypan blue. Preparative isoelectric focusing partially purified the MCA-F-specific antigen from crude C8Glu extracts into the pH 6.5-6.8 region of the gradient. Electrofocusing yielded 60% of the applied antigen activity and a fourfold to fivefold increase in specific activity. In addition, immunoprotective activity was obtained in 2% C8Glu extracts of MCA-F plasma membranes, confirming the membrane localization of the MCA-FTSTA. Three properties of C8Glu rendered it an attractive agent for the preparation of cell surface proteins: a nonionic character, large critical micellar concentration, and its capacity to extract antigens without complete membrane solubilization or cell disruption.
D000818:Animals; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D002462:Cell Membrane / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D005260:Female; D005354:Fibrosarcoma / Q000032:analysis; D005960:Glucosides; D006027:Glycosides; D006649:Histocompatibility Antigens / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D008748:Methylcholanthrene; D051379:Mice; D008809:Mice, Inbred C3H
D000951:Antigens, Neoplasm; D005960:Glucosides; D006027:Glycosides; D006649:Histocompatibility Antigens; C029237:tumor-associated transplantation antigen; C018619:octyl-beta-D-glucoside; D008748:Methylcholanthrene