Stability of globin mRNA in terminally differentiating murine erythroleukemia cells.
The stability of globin mRNA is terminally differentiating MEL cells has been reevaluated. Previously, it had been reported that globin mRNA has a half-life of approximately 17 hr in terminally differentiating MEL cells. We show that the previous measurements of this parameter were confounded by physical instability of differentiating MEL cells. By using culture conditions that physically stabilize end-stage cells we show that the stability of globin mRNA in terminally differentiating MEL cells is equal to the value observed for ribosomal RNA, a half-life greater than 60 hr.
D000818:Animals; D002460:Cell Line; D003470:Culture Media; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004920:Erythropoiesis; D005914:Globins; D006207:Half-Life; D007505:Iron-Dextran Complex / Q000494:pharmacology; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D003470:Culture Media; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012710:Serum Albumin, Bovine; D005914:Globins; D007505:Iron-Dextran Complex; D004121:Dimethyl Sulfoxide