title: Specific in vitro transcription of conalbumin gene is drastically decreased by single-point mutation in T-A-T-A box homology sequence.
abstract: A single-point mutation, consisting of a T-to-G transversion, was made in the third nucleotide of the conalbumin gene T-A-T-A-A-A-A homology sequence (the T-A-T-A or Goldberg-Hogness box). In an in vitro system, specific transcription of the mutant DNA was drastically decreased compared to the normal gene. This down-mutation is consistent with the idea that the T-A-T-A box is an important element for specific initiation of transcription.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002474:Cell-Free System; D002645:Chickens; D003207:Conalbumin / Q000235:genetics*; D004527:Egg Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D009154:Mutation; D009876:Operon; D014158:Transcription, Genetic
chemical_list: D004527:Egg Proteins; D003207:Conalbumin