The asparagine-linked sugar chains of plasma membrane glycoproteins of K-562 human leukaemic cells: a comparative study with human erythrocytes.
The paper electrophoretic pattern of the oligosaccharides released from the plasma membranes of K-562 cells by hydrazinolysis was quite different from that of human erythrocyte membranes. Bio-Gel P-4 column chromatography in combination with sequential exoglycosidase digestion of the neutral oligosaccharide fractions revealed that all those from K-562 cells are of the high mannose type, while those from erythrocytes are of large complex type structures. Studies of the acidic oligosaccharides indicated that none of those obtained from K-562 cells contained the beta-N-acetylglucosamine residue linked at the C-4 position of the beta-mannosyl residue of the trimannosyl core, which occurs in most of the asparagine-linked sugar chains of human erythrocytes. This indicates that the glucosaminyltransferase that forms the GlcNAc beta 1 leads to 4Man beta 1 leads to group has not been expressed in K-562 cells.
D001216:Asparagine; D002236:Carbohydrate Conformation; D002460:Cell Line; D004590:Electrophoresis, Paper; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis*; D006026:Glycoside Hydrolases; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D009439:Neuraminidase; D009844:Oligosaccharides
D006023:Glycoproteins; D009844:Oligosaccharides; D001216:Asparagine; D006026:Glycoside Hydrolases; D009439:Neuraminidase