title: Ganglioside profiles of metastases and of metastasizing and nonmetastasizing rat primary mammary carcinomas.
abstract: The possible relationship between ganglioside levels and ganglioside profiles in malignant tumors and the formation of metastasis was investigated by the analysis of gangliosides in metastasizing SMT-2A and nonmetastasizing MT-W9a mammary carcinomas as well as in metastases formed from SMT-2A tumors. The extracted lipid of SMT-2A tumors contained 3.3-fold more lipid-bound sialic acid than did that of MT-W9a tumors. THe differences were also substantial in the ganglioside profiles in these 2 tumors. Plasma membranes isolated from SMT-2A tumors also contained 1.8-fold more lipid-bound sialic acid than did plasma membranes from MT-W9a tumors. Ganglioside profiles in two types of SMT-2A secondary tumors were investigated. The lipid-bound sialic acid content was 1.5-fold higher in tumor nodules in the lung and 1.9-fold higher in axillary lymph node tumors than it was in primary SMT-2A tumors. The ganglioside pattern in these 2 secondary tumors generally reflected that found in SMT-2A: high levels of gangliosides containing three or four sialic acid molecules. The lung nodule retained its specificity with respect to lipid-bound sialic acid content and ganglioside pattern after the lung nodule was sequentially transplanted three times to the site of the original SMT-2A tumor growth.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D005732:Gangliosides / Q000378:metabolism*; D050356:Lipid Metabolism; D008175:Lung Neoplasms / Q000378:metabolism / Q000556:secondary; D008207:Lymphatic Metastasis / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism*; D009362:Neoplasm Metastasis / Q000503:physiopathology*; D051381:Rats; D011920:Rats, Inbred WF; D012794:Sialic Acids / Q000378:metabolism
chemical_list: D005732:Gangliosides; D012794:Sialic Acids