Localization of A protein in the RNA-A protein complex of RNA phage MS2.
The site of interaction of phage MS2 A protein on MS2 RNA was determined by analysing the base sequence of the RNA fragment which was released from the RNAase-resistant complex prepared by RNAase digestion of RNA-A protein complex. The result showed that there were two types of RNA fragment: one had a sequence which corresponded to the sequence of the 5'-side maturation region of MS2 RNA and the other corresponded to the sequence of the 3'-side untranslated region. These results were confirmed by a competition experiment in in vitro reconstitution system using f2 defective RNA, lacking about 30% of the 5'-side, and MS2 5FUra (5-fluorouracil) RNA, lacking about 35% of the 3'-side, as competitors. These results seem to indicate that the A protein is bound to at least two sites on MS2 RNA.
D001483:Base Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D009690:Nucleic Acid Conformation; D012317:RNA Phages / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D012260:Ribonucleases; D014764:Viral Proteins / Q000378:metabolism*
D012367:RNA, Viral; D014764:Viral Proteins; D012260:Ribonucleases