Detection of platelet-directed immunoglobin G in sera using the peroxidase-anti-peroxidase (PAP) slide technique.
An immunohistochemical procedure for the detection of immunoglobulin G adherent to platelets is described. The peroxidase anti-peroxidase method is used to detect antibody activity directed against platelets from normal donors in the sera from 305 individuals. These subjects were divided into three groups: group 1, patients referred for tissue typing; group 2, healthy normal females; group 3, healthy normal males. In group 1, 28% of the sera were found to be positive; in most of these a history of prior transfusions was obtained. In group 2, 7.4% were found to be positive, most having previous pregnancies. Only 1% were found to be positive in group 3, and no reason for presensitization was found. Results from the indirect immunofluorescence technique served as a control and as a means to compare the sensitivity. Under the conditions chosen, the peroxidase anti-peroxidase test was two to eight times more sensitive than the immunofluorescence technique. Specificity of the peroxidase anti-peroxidase technique was demonstrated using a monospecific anti-PLA1 antiserum. It is concluded that the peroxidase anti-peroxidase slide technique may be a useful tool in the study of platelet-related immunophenomena.
D000017:ABO Blood-Group System; D000042:Absorption; D000818:Animals; D001792:Blood Platelets / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006041:Goats; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G; D008297:Male; D011247:Pregnancy; D011693:Purpura / Q000276:immunology; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell
D000017:ABO Blood-Group System; D006680:HLA Antigens; D007074:Immunoglobulin G; D011947:Receptors, Antigen, B-Cell