title: Use of an enzyme immunoassay with protein A for rabies antigen and antibody determination.
abstract: The rabies antigen quantitation reported here is based on the principle of an enzyme immuno micro assay (EIA) using antigen coated polystyrene microtiter plates. In a first step antibodies of known specificity are partially blocked by the antigen to be titrated; in a second step the free remaining antibodies are determined by EIA. Antirabies vaccines, purified virus or rabies glycoprotein were assayed by that micro-method in comparison with the double neutralization test in tissue culture. Moreover, we report results obtained by EIA on the rate of antigen bonding to a solid carrier in order to prepare immunoadsorbents and the usefulness of EIA to monitor specific immunoglobulin elution.
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007164:Immunosorbents; D009500:Neutralization Tests; D011819:Rabies Vaccines / Q000276:immunology*; D011820:Rabies virus / Q000276:immunology*; D013205:Staphylococcal Protein A; D014764:Viral Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D007164:Immunosorbents; D011819:Rabies Vaccines; D013205:Staphylococcal Protein A; D014764:Viral Proteins