Characterization of in vitro T lymphocyte colonies from normal mouse spleen cells: colonies containing cytotoxic lymphocyte precursors.
The functional activity of T cell colonies grown from normal murine spleen cells has been examined. Up to 75% of the T cell colonies contain cytotoxic lymphocyte precursors (CLP). One out of three colonies contains CLP, which can respond to a given foreign H-2 haplotype. The CLP from the T cell colonies appear identical to the CLP in lymph node tissues in their degree of cross-reactivity and requirement for accessory cells. The effector cells produced were shown to be T lymphocytes. Karyotypic analysis of the cells in colonies grown from a mixture of T6-marked and normal spleen cells indicated that individual colonies were formed from an initial small aggregate of cells. Radiation survival data indicated that the colony-forming unit contains three or fewer proliferating cells.
D000818:Animals; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D002999:Clone Cells / Q000166:cytology; D003602:Cytotoxicity, Immunologic; D005260:Female; D006183:H-2 Antigens; D006238:Haploidy; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008819:Mice, Nude; D013154:Spleen / Q000166:cytology*; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D006183:H-2 Antigens