Cation complexed DNA. A novel class of immunoadsorbents for anti-DNA antibodies.
DNA can be precipitated as complexes with metal and organic cations. We have prepared these products using divalent metal cations (Cu2+, Mn2+, Ni2+, Zn2+, and Co2+) and ditetrazolium ions (blue tetrazolium and neotetrazolium). They are effective adsorbents, in vitro, for anti-nDNA antibodies and in reducing the titer of antinuclear antibodies in the sera from patients with autoimmune disease. They selectively adsorb most of the antinuclear components from a mixture of antimitochondrial and antinuclear antibodies.
D000818:Animals; D000974:Antibodies, Antinuclear; D002417:Cattle; D003035:Cobalt; D003300:Copper; D004247:DNA / Q000276:immunology*; D006801:Humans; D007164:Immunosorbents; D008345:Manganese; D008928:Mitochondria / Q000276:immunology; D009532:Nickel; D009580:Nitroblue Tetrazolium; D011817:Rabbits; D015032:Zinc
D000974:Antibodies, Antinuclear; D007164:Immunosorbents; D009580:Nitroblue Tetrazolium; D003035:Cobalt; D008345:Manganese; D003300:Copper; D009532:Nickel; D004247:DNA; D015032:Zinc