Antigen-specific human T-cell hybridomas with helper activity.
Human T cell hybridomas were produced by fusing the hypoxanthine phosphoribosyltransferase-deficient line of the human T cell lymphoma Jurkat with a continuous line of normal human T cells specific for tetanus toxoid (TeT). The hybridomas were selected for their ability to produce interleukin 2 after exposure to TeT on semiautologous monocytes and for their ability to bind to TeT-pulsed semiautologous monocytes. These antigen-specific T hybridomas demonstrated potent helper activity for semiautologous B cells as determined by the production of high levels of anti-TeT antibody in vitro.
D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007376:Interleukin-2 / Q000096:biosynthesis; D008211:Lymphocyte Cooperation; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology
D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D013745:Tetanus Toxoid