The E. coli Pol A-1 assay: a quantitative procedure for diffusible and non-diffusible chemicals.
A procedure is described for determining the preferential killing of DNA repair-deficient E. coli. Dilute suspensions of DNA repair-proficient and -deficient bacteria are exposed to a graded series of test chemical concentrations. Survivors are determined by enumeration on agar plates. An expression used to express preferential killing (or lack thereof) is shown to be dose-dependent and potentially useful for determining genotoxic potencies.
D004260:DNA Repair; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics*; D005821:Genetic Techniques; D009153:Mutagens; D010641:Phenotype
D009153:Mutagens