Production of low glucose serum for quality control and evaluation of yeast treatment, lyophilization and storage conditions on serum constituents.
In order to provide quality control serum in the hypoglycemic range, pooled human serum was treated with yeast. Yeast destroyed about 50% of the serum glucose in about 4 1/2 hrs. The yeast-treated serum remained suitable for quality control of the most commonly analyzed clinical chemistry constituents which showed only very little change in most cases. Serum triglycerides were increased by about 40% and bilirubin decreased by about 20% during the treatment. Lyophilization of serum samples (yeast-treated or not) resulted in significant decreases of some enzymes activities. Exposure at 22 degrees C of samples lyophilized (7 days) and non-lyophilized (4 days) resulted in practically no change except for certain enzymes. No significant differences were observed in the clinical chemistry measurements including glucose one month and three months after preparation of samples lyophilized (stored in a refrigerator) and non-lyophilized (store in a freezer).
D001663:Bilirubin / Q000097:blood; D001769:Blood; D001786:Blood Glucose / Q000378:metabolism*; D004355:Drug Stability; D004356:Drug Storage; D004798:Enzymes / Q000097:blood; D005374:Filtration; D005612:Freeze Drying; D006801:Humans; D007003:Hypoglycemia / Q000097:blood; D011786:Quality Control; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D004798:Enzymes; D014280:Triglycerides; D001663:Bilirubin