Patterns of late replication in X chromosomes of human lymphoid cells.
Two predominant patterns of late X replication were observed in both short-term and established human lymphoid cultures. One pattern was found in a minority of short-term cultured T-cell metaphases, in most lectin-stimulated B cells, and, with minor variations, in established B-cell lines. In these cells, DNA replication terminated in the distal part of the long arm of the late X. A different pattern was found in the majority of lectin-simulated T cells and in the T-cell line CCRF-CEM. These cells exhibited terminal replication in a region of the long arm of the late X that was nearer to the centromere. It is speculated that the variations in replication patterns correlate with phenotypic and functional characteristics of human lymphoid subsets.
D001402:B-Lymphocytes / Q000502:physiology*; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D004261:DNA Replication; D005260:Female; D006801:Humans; D007621:Karyotyping; D008213:Lymphocyte Activation; D008677:Metaphase; D012730:Sex Chromosomes; D013601:T-Lymphocytes / Q000502:physiology*; D014960:X Chromosome