Characterization of the cell wall and cell wall proteins of Chromatium vinosum.
Highly purified cell walls of Chromatium vinosum were isolated by differential centrifugation, with or without Triton X-100 extraction. The isolated material had a protein composition similar to that of cell walls obtained by sucrose density gradient centrifugation. Twenty-two proteins were reproducibly detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A 42-kilodalton protein was shown to account for 65% of the total cell wall protein. The majority of cell wall proteins were solubilized in sodium dodecyl sulfate at room temperature; however, they existed as high-molecular-weight complexes unless heated to 45 degrees C or above. The cell wall contained one heat-modifiable protein which migrated with an apparent molecular weight of 37,400 when solubilized at 70 degrees C or below, but which migrated with an apparent molecular weight of 52,500 if solubilized at 100 degrees C. The electrophoretic mobility of three proteins was modified by 2-mercaptoethanol. The majority of C. vinosum cell wall proteins had isoelectric points between pH 4.5 and 5.5, and the 42-kilodalton protein focused at pH 4.9. No proteins were detected which were analogous to the lipoprotein or peptidoglycan-associated proteins of the Enterobacteriaceae. Nearest-neighbor analysis with a reducible, cross-linking reagent indicated that three proteins, including the 42-kilodalton protein, associated with themselves. Most of the cell wall proteins were partially accessible to proteases in both intact cells and isolated cell walls. Protease treatment of the whole cell or isolated cell wall digested approximately an 11,000-molecular-weight portion of the 42-kilodalton protein.
D001426:Bacterial Proteins / Q000032:analysis*; D002458:Cell Fractionation; D002462:Cell Membrane / Q000032:analysis; D002473:Cell Wall / Q000032:analysis; D002844:Chromatium / Q000032:analysis / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000032:analysis*; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000494:pharmacology; D010457:Peptidoglycan / Q000032:analysis
D001426:Bacterial Proteins; D008565:Membrane Proteins; D010457:Peptidoglycan; D010447:Peptide Hydrolases