A diffusable clastogenic factor in ataxia telangiectasia.
Cocultivation of plasma and lymphocytes from ataxia telangiectasia (AT) patients with those of normal individuals resulted in a significant increase in chromosomal damage in the normal cells. Tissue culture medium used to cultivate AT skin fibroblasts also significantly increased chromosome breakage in PHA-stimulated normal lymphocytes. This clastogenic effect was maximal when the "conditioned medium" was 8-9 days old. A similar effect was not observed with medium derived from normal skin fibroblasts. These observations suggest the presence of a clastogenic factor in the plasma of AT patients which may also be produced by AT skin fibroblasts in culture.
D001260:Ataxia Telangiectasia / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003470:Culture Media; D004058:Diffusion; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008297:Male; D009153:Mutagens / Q000009:adverse effects; D010949:Plasma / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*
D003470:Culture Media; D009153:Mutagens