Implantation of mouse histocompatibility antigens into membranes of cultured tumor cells.
Membranes of murine lymphoma cells expressing H-2a antigens were isolated, purified and co-reconstituted with isolated Sendai virus envelopes according to a previously published procedure (Volsky, D.J. et al., Proc. Natl. Acad. Sci. USA 1979. 76: 5440.). The resulting hybrid H-2a/Sendai virus envelope vesicles (SH-2a vesicles) were capable of binding to and fusing with mouse lymphoma cells. The fusion resulted in the implantation of H-2a antigens into membranes of target cells, as demonstrated by the presence of serologically active antigens on cultured cells 8 and 16 h after implantation.
D000818:Animals; D000954:Antigens, Surface; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens; D006649:Histocompatibility Antigens; D007700:Kinetics; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D011092:Polyethylene Glycols / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology
D000954:Antigens, Surface; D006183:H-2 Antigens; D006649:Histocompatibility Antigens; D011092:Polyethylene Glycols