Proliferation activity and bacteriostatic potential of human blood monocytes, macrophages in pleural effusions, ascites, and of alveolar macrophages.
Human blood monocytes, macrophages from pleural effusions, ascites, and alveolar macrophages obtained by bronchopulmonary lavage were investigated. The proliferative activity of these cells was determined by the 3H-thymidine labeling index in vitro (3H-TDR L.I.). The bacteriostatic capacity was measured by the potential of the cells to block DNA-synthesis of proliferating Escherichia coli after phagocytosis. In most cases 3H-TDR L.I. of blood monocytes, macrophages from pleural effusions and ascites was less than 1%. However, macrophages of some patients with neoplastic diseases exhibited 3H-TDR L.I. between 4.0--9.6%. This probably reflected the action of factors, possibly lymphokines, which stimulated macrophage proliferation. In contrast, alveolar macrophages seemed to have almost totally lost their proliferative potential. The bacteriostatic capacity of blood monocytes proved to be significantly lower than that of macrophages. This demonstrates the functional immaturity of blood monocytes. In all type of macrophages investigated the bacteriostatic power was very high. No differences could be detected either between macrophages of different sources or between macrophages of benign, inflammatory, or malignant diseases.
D001201:Ascites; D002455:Cell Division; D004269:DNA, Bacterial / Q000096:biosynthesis; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D008264:Macrophages; D009000:Monocytes; D010996:Pleural Effusion; D011650:Pulmonary Alveoli / Q000648:ultrastructure
D004269:DNA, Bacterial