Studies of pancreatic nodule induction and DNA damage by D-azaserine.
The ability of the D-isomer of azaserine to induce atypical acinar cell nodules (AACN) in pancreas and to cause DNA damage in pancreas and liver was evaluated. Rats were injected with equivalent doses of D- or L-azaserine and numbers of AACN were counted after 4 months. DNA damage in pancreas and liver of rats treated in vivo, and in pancreatic acinar cells treated in vitro with D- or L-azaserine was determined by alkaline elution. Results show that D-azaserine does not significantly induce AACN in pancreas, nor does it cause extensive DNA damage in comparison with L-azaserine, suggesting that the differential effect of the 2 isomers is related to stereospecificity in either transport or metabolism.
D000818:Animals; D001377:Azaserine / Q000378:metabolism / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D004247:DNA; D008099:Liver / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D011917:Rats, Inbred Lew; D013237:Stereoisomerism
D002273:Carcinogens; D001377:Azaserine; D004247:DNA