title: Detergent extraction of membrane proteins related to the action of antidiuretic hormone.
abstract: Antidiuretic hormone (ADH) induces, in the apical plasma membrane of target cells, the insertion of intramembranous particle aggregates that probably contain water channels. A mild attack of this membrane by a polyoxyethylene nonylphenyl detergent, which reversibly depressed ADH-induced water permeability, has been found to modify aggregate structure while extracting additional proteins. This simple procedure could be a valuable approach to the problem of aggregate isolation and characterization.
mesh_terms: D000818:Animals; D007700:Kinetics; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011092:Polyethylene Glycols; D011893:Rana esculenta; D013501:Surface-Active Agents / Q000494:pharmacology*; D001743:Urinary Bladder / Q000378:metabolism*; D014667:Vasopressins / Q000494:pharmacology*
chemical_list: D008565:Membrane Proteins; D013501:Surface-Active Agents; D014667:Vasopressins; D011092:Polyethylene Glycols