Induction of cell-mediated cytotoxic self responses by epidermal cells modified with a haptenic sulphydryl reagent.
Murine epidermal cells (EC) act as stimulator cells in the generation of allogeneic cytotoxic T lymphocytes (CTL) in cell-mediated lympholysis (CML) and are suitable targets for allogeneic and hapten-self CTL. To analyse the role of EC in the generation of and recognition by anti-self CTL, syngeneic hapten-modified murine EC were used as in vivo and in vitro stimulating populations and as target cells in a hapten-self CML system. Epidermal cells were modified with the sulphydryl-reactive haptenic reagent N-iodoacetyl-N'-(5-sulphonic-1-naphthyl)ethylenediamine (I-AED). C3H.SW (H-2b) AED-self CTL responses were generated by stimulation with syngeneic AED-modified EC and were readily demonstrated when tested on syngeneic hapten-modified EC. These CML responses were hapten-specific and H-2 restricted. No substantial difference was detected in the ability of AED-modified EC and spleen cells (SC) to stimulate the generation of secondary AED-self CTL. Cold target inhibition experiments with hapten-modified EC and SC blockers did not reveal tissue-specific recognition of hapten-modified EC or SC targets by AED-self CTL. These findings demonstrate that hapten-modified EC, when used for priming in vivo and subsequently for in vitro sensitization, can induce hapten-specific self CTL that are reactive against syngeneic hapten-modified EC.
D000818:Animals; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D005260:Female; D006183:H-2 Antigens; D006241:Haptens; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008954:Models, Biological; D009282:Naphthalenesulfonates / Q000276:immunology / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology
D006183:H-2 Antigens; D006241:Haptens; D009282:Naphthalenesulfonates; D013439:Sulfhydryl Reagents; C006421:1,5-I-AEDANS