Human skin cells synthesize HLA-DR molecules of the same charge and molecular weight as those synthesized by autologous lymphocytes.
Between 5 and 14% of human skin cells, separated by trypsinization, express HLA-DR antigen as detected by indirect immunofluorescence with monoclonal anti-DR antibody. To determine the source and structure of these DR molecules, skin cell suspensions were biosynthetically labeled with 35S-methionine and the radiolabeled DR molecules were analyzed by the method of two-dimensional polyacrylamide gel electrophoresis. The results indicated the presence of heavy, intermediate, and light chains indistinguishable from those of B lymphocyte DR. Gels in which DR immunoprecipitates from skin cells and autologous lymphocytes were run simultaneously confirmed this finding. Thus, skin cells and lymphocytes synthesize HLA-DR antigen of identical charge and molecular weight.
D001402:B-Lymphocytes / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004727:Endothelium / Q000166:cytology; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D066298:In Vitro Techniques; D008970:Molecular Weight; D012867:Skin / Q000166:cytology / Q000276:immunology*
D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II