Generation of effector cells from T cell subsets. III. Synergy between Lyt-1 and Lyt-123/23 lymphocytes in the generation of H-2-restricted and alloreactive cytotoxic T cell.
Lyt-123/23 and Lyt-1 T cell subsets, positively selected by separation on the fluorescence-activated cell sorter, were tested in vitro for their role in the generation of H-2-restricted and alloreactive cytotoxic T cells. It is shown that in the proliferation assay, both T cell subsets responded equally well to H-2 and non-H-2 antigens (H-Y), respectively. In contrast, none of the selected Lyt subsets, but only the mixed population containing Lyt-1 and Lyt-123/23 lymphocytes gave rise to both H-2-restricted (anti H-Y, anti-trinitrophenyl) and alloreactive (anti-H-2) cytotoxic lymphocytes. The data imply an essential role of Lyt-1 cells as inducers or helpers in the generation of all cytotoxic lymphocytes from their precursors in the Lyt-123/23 pool.
D000818:Animals; D000950:Antigens, Ly / Q000276:immunology*; D000954:Antigens, Surface / Q000502:physiology*; D002469:Cell Separation / Q000379:methods; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens; D006182:H-Y Antigen / Q000276:immunology*; D007111:Immunity, Cellular; D007518:Isoantibodies; D007519:Isoantigens; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*
D000950:Antigens, Ly; D000954:Antigens, Surface; D006183:H-2 Antigens; D006182:H-Y Antigen; D007518:Isoantibodies; D007519:Isoantigens; C029805:Lyt antibodies