Murine T cell lines that help induction and generation of allospecific cytotoxic T cells from thymocytes.
Murine T cell lines that help induction and generation of allospecific cytotoxic T cells (CTL) from thymocytes cultured together with allogeneic stimulator cells have been established. It was done by successively culturing T cell blasts obtained from mixed lymphocyte cultures (MLC) in the medium supplemented with T cell growth factor (TCGF). The helper function of the long-term cultured T cells was antigen specific, radiation resistant, and not H-2 restricted. The helper function has been retained for more than 8 mo by the cells continually proliferating in the TCGF-supplemented medium. The T stimulated responsiveness of thymocytes to T cell mitogens. CTL activity seen in the original MLC waned rapidly during successive culture of this T cell line in TCGF medium T cell blasts from MLC successively cultured in continual presence of TCGF and the stimulator alloantigens retained strong CTL activity against the allogeneic stimulator cells for about 3 mo. All these T cell lines proliferating in TCGF medium expressed Thy 1 antigen and showed the appearance of a large T cell blast. These T cell lines could not proliferate in the absence of TCGF and lost responsiveness to T cell mitogens intrinsically.
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D006133:Growth Substances / Q000494:pharmacology; D007519:Isoantigens; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008934:Mitogens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D013997:Time Factors
D006133:Growth Substances; D007519:Isoantigens; D008934:Mitogens