Determination of fluoxetine and norfluoxetine in plasma by gas chromatography with electron-capture detection.
This gas-chromatographic method for assay of fluoxetine and norfluoxetine in human plasma involves extraction of the drugs and use of a 63Ni electron-capture detector. The linear range of detection is 25 to 800 micrograms/L for each drug. Overall precision (CV) in the concentration range of 10 to 100 micrograms/L for both drugs was approximately 10%. Accuracy (relative error) in the same concentration range was approximately +10%. None of the commonly prescribed antidepressants or tranquilizers that we tested interfere with the assay.
D002849:Chromatography, Gas / Q000379:methods; D004583:Electrons; D005473:Fluoxetine / Q000031:analogs & derivatives / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D011437:Propylamines / Q000097:blood*
D011437:Propylamines; D005473:Fluoxetine; C036139:norfluoxetine