B-lymphocyte responses to trinitrophenyl-conjugated Ficoll: requirement for T lymphocytes and Ia-bearing adherent cells.
These studies were done to characterize the cellular requirements for B-lymphocyte responses to the haptenated polysaccharide trinitrophenyl-conjugated Ficoll. By using an in vitro microculture system, it was demonstrated that hapten-specific anti-trinitrophenyl-conjugated Ficoll plaque-forming cell responses by B lymphocytes require Ia-bearing adherent accessory cells and Thy 1+ Lyt 1+2- nylon wool-nonadherent (T) lymphocytes. Such T cells could be primed in vivo with nonderivatized Ficoll to show carrier-specific helper cell function for derivatized Ficoll responses in vitro. The implications of these findings for our understanding of b-lymphocyte triggering by so-called T-independent antigens are discussed.
D000818:Animals; D000950:Antigens, Ly / Q000032:analysis; D000955:Antigens, T-Independent; D001402:B-Lymphocytes / Q000276:immunology*; D002448:Cell Adhesion; D002478:Cells, Cultured; D005260:Female; D005362:Ficoll / Q000276:immunology; D000949:Histocompatibility Antigens Class II; D008211:Lymphocyte Cooperation; D051379:Mice; D009000:Monocytes / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D000950:Antigens, Ly; D000955:Antigens, T-Independent; D000949:Histocompatibility Antigens Class II; D014301:Trinitrobenzenes; D005362:Ficoll