Regulatory factors in human serum: effect on murine T cells.
Effect of lymphocyte E rosette stimulatory and inhibitory factors from human serum on murine T cells was studied. It was shown that the stimulatory factor (a polypeptide, temperature sensitive, with a molecular weight about 20,000, isolated from healthy individuals) interacted, in vitro, with murine spleen T cell precursors and generated formation of Thy-1,2+ cells. The cells generated were also active in GvH reaction. The stimulatory factor induced, in vitro, formation of cortisone-resistant thymocytes. Unfractionated pool of spleen cells treated with the inhibitory factor (a beta-lipoprotein isolated from sera of humans with active pulmonary tuberculosis) showed lower number of Thy-1,2+ cells, and lower activity in GvH reaction. However, when the spleen cells were subsequently treated with the stimulatory factor, the initial number of Thy-1,2+ cells was restored. It was confirmed that the factors did not interact with the target cells in the presence of prostaglandin inhibitors.
D000276:Adjuvants, Immunologic; D000818:Animals; D000941:Antigens / Q000096:biosynthesis; D002478:Cells, Cultured; D006087:Graft vs Host Reaction; D006168:Guinea Pigs; D006801:Humans; D007166:Immunosuppressive Agents; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000097:blood*; D051379:Mice; D010455:Peptides / Q000097:blood* / Q000494:pharmacology; D012397:Rosette Formation; D013154:Spleen; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology
D000276:Adjuvants, Immunologic; D000941:Antigens; D007166:Immunosuppressive Agents; D008077:Lipoproteins, LDL; D010455:Peptides