title: Production of monocyte/macrophage colony-stimulating factor by preadipocyte cell lines derived from murine marrow stroma.
abstract: Three preadipocyte cell lines that have been independently derived from bone marrow stroma (Lanotte et al, 1982) have been tested for their capacity to produce granulocyte, macrophage, and erythroid colony-stimulating factors (CSFs). All elaborated colony-stimulating material that was active upon adult mouse marrow granulocyte/macrophage colony-forming cells (M-CFC) but not foetal liver GM-CFC. The major activity was characterised as a monocyte-macrophage colony-stimulating factor (M-CSF), and the pattern of colony stimulation was similar to that seen after addition of highly purified L-cell CSF. Furthermore, the stimulating activity was specifically neutralised by rabbit anti-L cell CSF antibodies. No evidence was found for stimulation of multipotential or erythroid colony-forming cells, only few granulocytic colonies were detected, and the stimulating activity had no mouse strain restriction. All cell lines produced large quantities of M-CSF; however, the production was found to be modulated during the adipogenesis process. A peak in M-CSF production corresponded to the period of growth arrest after confluence of the stromal cells was reached and when adipocyte maturation was at an early stage. A marked depression in M-CSF secretion was associated with the final steps of adipocyte maturation.
mesh_terms: D000273:Adipose Tissue / Q000502:physiology*; D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D002454:Cell Differentiation; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D003470:Culture Media; D007700:Kinetics; D008264:Macrophages / Q000502:physiology; D051379:Mice; D009000:Monocytes / Q000502:physiology
chemical_list: D003115:Colony-Stimulating Factors; D003470:Culture Media