Establishment and characterization of a human acute monocytic leukemia cell line (THP-1).
A human leukemic cell line (THP-1) cultured from the blood of a boy with acute monocytic leukemia is described. This cell line had Fc and C3b receptors, but no surface or cytoplasmic immunoglobulins. HLA haplotypes of THP-1 were HLA-A2, -A9, -B5, -DRW1 and -DRW2. The monocytic nature of the cell line was characterized by: (1) the presence of alpha-naphthyl butyrate esterase activities which could be inhibited by NaF; (2) lysozyme production; (3) the phagocytosis of latex particles and sensitized sheep erythrocytes; and (4) the ability to restore T-lymphocyte response to Con A. The cells did not possess Epstein-Barr virus-associated nuclear antigen. These results indicate that THP-1 is a leukemia cell line with distinct monocytic markers. During culture, THP-1 maintained these monocytic characteristics for over 14 months.
D002460:Cell Line; D003179:Complement C3b; D004950:Esterases / Q000032:analysis; D006801:Humans; D007223:Infant; D007948:Leukemia, Monocytic, Acute / Q000473:pathology*; D008213:Lymphocyte Activation; D008297:Male; D009000:Monocytes / Q000502:physiology / Q000648:ultrastructure; D009113:Muramidase / Q000096:biosynthesis; D010587:Phagocytosis; D011951:Receptors, Complement / Q000032:analysis; D011961:Receptors, Fc / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology
D011951:Receptors, Complement; D011961:Receptors, Fc; D003179:Complement C3b; D004950:Esterases; D009113:Muramidase