title: Staphylococcus aureus cowan I and Branhamella catarrhalis as B lymphocyte mitogens. Culture conditions for optimal DNA synthesis and selective stimulation of human B lymphocytes.
abstract: Staphylococcus aureus Cowan I and Branhamella catarrhalis that selectively stimulate human B lymphocytes to increased DNA synthesis in serum-free culture media were shown to do so also in 3 day cultures in medium containing human serum or albumin. After this time the transformed cells expressed little or no surface-bound immunoglobulin and did not form rosettes with sheep erythrocytes. When lymphocytes were cultured with bacterial mitogens in the presence of serum or albumin for more than 3 days transformed T cells appeared. The bacteria induced antibody production mainly of IgM class in lymphocytes cultured in albumin supplemented medium.
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis*; D005615:Freezing; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D008934:Mitogens / Q000494:pharmacology*; D009343:Neisseria / Q000276:immunology; D012397:Rosette Formation; D012709:Serum Albumin / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000276:immunology; D013936:Thymidine / Q000378:metabolism; D014168:Transferrin / Q000494:pharmacology
chemical_list: D003470:Culture Media; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008934:Mitogens; D012709:Serum Albumin; D014168:Transferrin; D004247:DNA; D013936:Thymidine