The effect of phorbol-myristate acetate and concanavalin A on the growth of interleukin-2-dependent T-cell lines.
We tested the effect of phorbol myristate acetate (PMA) and of Con A on the growth of two Il-2 dependent T cell-lines. Il-2 dependent growth, as measured by 3H-TDR incorporation or viable cell counts was enhanced by PMA (5-100 ng/ml) and inhibited by Con A. These effects were only reproducible in the presence of intermediate concentrations of Il-2 containing supernatant. The enhancing effect of PMA was probably due to a positive influence on the replication rate since the drug had no visible effect on cell survival and since no evidence was found for the induction of Il-2 production by the cell-lines.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000494:pharmacology*; D010704:Phorbols / Q000494:pharmacology*; D051381:Rats; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
D007376:Interleukin-2; D008222:Lymphokines; D010704:Phorbols; D003208:Concanavalin A; D013755:Tetradecanoylphorbol Acetate; D013936:Thymidine