title: Association of myosin light chain kinase with lymphocyte membrane-cytoskeleton complex.
abstract: A specific antibody against myosin light chain kinase (MLCK) was used to identify the presence of a Ca2+-calmodulin-activated MLCK in mouse 1-lymphoma cells. With a double immunofluorescence technique, MLCK was determined to be accumulated directly under Con A-capped structures in a manner similar to that of previously described accumulation of actomyosin. The lymphocyte MLCK was phosphorylated in the uncapped cell and, by immunoprecipitation with a specific MLCK antibody, was shown to possess a Mr of 130,000. The MLCK was also found to constitute a major fraction of the phosphoproteins present in the plasma membrane associated-cytoskeleton. Myosin light chain kinase catalyzed the phosphorylation of both endogenous lymphocyte myosin light chains and those from smooth and skeletal muscle. The enzyme activity was dependent on the presence of Ca2+-calmodulin and was inhibited by the calmodulin-binding drug, trifluoperazine. These data suggest that the membrane-cytoskeleton-associated MLCK activity may be important in regulation of the actinmyosin contraction which is believed to be required for the collection of surface receptors into capped structures.
mesh_terms: D000818:Animals; D002147:Calmodulin / Q000502:physiology; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D003208:Concanavalin A; D003599:Cytoskeleton / Q000201:enzymology*; D007152:Immunologic Capping; D008223:Lymphoma; D051379:Mice; D009219:Myosin-Light-Chain Kinase; D009218:Myosins / Q000378:metabolism; D011494:Protein Kinases / Q000032:analysis* / Q000378:metabolism; D013601:T-Lymphocytes / Q000201:enzymology*
chemical_list: D002147:Calmodulin; D003208:Concanavalin A; D011494:Protein Kinases; D009219:Myosin-Light-Chain Kinase; D009218:Myosins