Multiple selective media for the isolation of anaerobic bacteria from clinical specimens.
Using fresh clinical material, a comparison of a number of anaerobic selective media was made. For Gram-negative anaerobes nalidixic acid tween agar (NAT), neomycin agar (NA), and neomycin-vancomycin agar (NVA) all performed equally well. Kanamycin-containing media were more inhibitory to all Gram-negative anaerobes other than Bacteroides fragilis and B. melaninogenicus. When the recovery of Gram-positive anaerobes was examined NAT performed better than any of the other selective media used. No single selective medium could recover all anaerobes. Better isolation was achieved using a combination of two selective media (the best combinations being NAT and NVA or NAT and NA). Only a combination of three selective media gave the maximum recovery of anaerobes in this study (NAT, NVA, and NA or KA).
D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D003470:Culture Media; D005260:Female; D006089:Gram-Negative Anaerobic Bacteria / Q000302:isolation & purification; D006801:Humans; D009268:Nalidixic Acid; D009355:Neomycin; D011136:Polysorbates; D014640:Vancomycin
D003470:Culture Media; D011136:Polysorbates; D009268:Nalidixic Acid; D014640:Vancomycin; D009355:Neomycin