Fibrin membrane endowed with biological function. V. Multienzyme complex of uricase, catalase, allantoinase and allantoicase.
The enzymes (uricase (EC 1.7.3.3), allantoinase (EC 3.5.3.4), and allantoicase (EC 3.5.2.5) which participate in degradation of purine bases, were embedded separately in fibrin membranes formed by fibrinogen-fibrin conversion with thrombin. All of these enzymes together with catalase were also embedded in a single fibrin membrane to make an immobilized multienzyme complex. The multienzyme complex in fibrin membrane thus prepared had an ability of degradation of uric acid to urea and glyoxylic acid via allantoin and allantoic acid. The stability of immobilized uricase or catalase embedded in fibrin membrane upon lyophilization was also tested in a comparison with nonimmobilized enzymes.
D000085:Acetates; D000481:Allantoin; D000581:Amidohydrolases / Q000378:metabolism*; D000818:Animals; D002175:Candida / Q000201:enzymology; D002374:Catalase / Q000378:metabolism*; D002417:Cattle; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005337:Fibrin; D005340:Fibrinogen; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D009097:Multienzyme Complexes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D014503:Urate Oxidase / Q000378:metabolism*; D014508:Urea / Q000031:analogs & derivatives; D014512:Ureohydrolases / Q000378:metabolism*
D000085:Acetates; D004800:Enzymes, Immobilized; D009097:Multienzyme Complexes; D000481:Allantoin; D014508:Urea; D005337:Fibrin; D005340:Fibrinogen; D002374:Catalase; D014503:Urate Oxidase; D000581:Amidohydrolases; C019659:allantoinase; D014512:Ureohydrolases; C019658:allantoicase