Production of human hybridomas secreting antibodies to measles virus.
Monoclonal antibodies against a variety of antigens can be produced using techniques of somatic cell hybridization between cells of rodent myeloma lines and B cells derived from animals immunized against a given antigen. However, because of the monoclonal antibodies secreted by these hybridomas are of rodent origin, their use in human immunotherapy is limited. Thus the production of B-cell hybrids that secrete human monoclonal antibodies may be of considerable value. We have hybridized a hypoxanthine phosphoribosyl transferase (HPRT)-deficient human B-cell line derived from a patient suffering from multiple myeloma with peripheral lymphocytes obtained from a patient with subacute sclerosing panencephalitis (SSPE). These hybridomas were found to secrete human IgM specific for measles virus nucleocapsids.
D000914:Antibodies, Viral / Q000096:biosynthesis*; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology; D002213:Capsid / Q000276:immunology; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology; D008459:Measles virus / Q000276:immunology*; D009194:Myeloma Proteins / Q000276:immunology; D013344:Subacute Sclerosing Panencephalitis / Q000276:immunology
D000914:Antibodies, Viral; D009194:Myeloma Proteins