Effects of anti-oxytocin serum in Brattleboro rats.
Brattleboro rats were housed singly in metabolic cages and continuously infused with saline. Timed urine collections were analyzed for Na+ and K+; oxytocin (OT)-like material was estimated by RIA. OT infusions reduced urine flow and increased Na+ output. Urinary Na+/K+ ratios rose during OT infusion, falling below control values after infusion. Passive immunization with an OT antiserum reduced but did not prevent these renal effects of OT. In a second experiment, immunization had no effect on daily urine volume or Na+ output, even though high titers were achieved and excretion of endogenous OT-like material was reduced. We conclude that endogenous OT has little effect on Na+ excretion in these rats, although pharmacological doses of OT produced their known natriuretic effect.
D000818:Animals; D003919:Diabetes Insipidus / Q000503:physiopathology; D004231:Diuresis / Q000187:drug effects; D007116:Immunization, Passive; D009318:Natriuresis / Q000187:drug effects; D010121:Oxytocin / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D011188:Potassium / Q000652:urine; D051381:Rats; D011910:Rats, Brattleboro / Q000502:physiology*; D011922:Rats, Mutant Strains / Q000502:physiology*
D010121:Oxytocin; D011188:Potassium