In vivo and in vitro sugar transport in frog intestine.
In the frog intestine, both in vitro and in vivo, experiments were carried out in order to increase knowledge of the mechanism of sugar exit across the basolateral membrane of the enterocyte. The frog intestine was chosen because it lacks crypt cells and, consequently, any external fluid circuit mechanism during sugar transport can be avoided. Therefore, the sugar concentration in the absorbate collected on the serosal side is likely to be similar to that present underneath the basolateral membrane of the enterocyte. Under this condition, cell and absorbate sugar concentrations are similar; yet there is a concomitant net transintestinal sugar transport. Moreover, in in vivo experiments a net transintestinal sugar transport takes place even against a concentration difference. These results suggest that sugar exit across the basolateral membrane is not simply due to a chemically facilitated diffusion.
D019325:3-O-Methylglucose; D000818:Animals; D001693:Biological Transport, Active; D050260:Carbohydrate Metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D008757:Methylglucosides / Q000378:metabolism; D011893:Rana esculenta
D008757:Methylglucosides; D019325:3-O-Methylglucose