Microspectrograpic analysis of formaldehyde-induced fluorescence in the quail adenohypophysis after injection of L-dopa and 5-hydroxytryptophan.
The spectral distribution of the formaldehyde-induced fluorescence emitted by model solutions and by adenohypophpyses after intraperitoneal injection of L-dopa or 5-hydroxytryptophan was analyzed microspectrographically. Based on previously reported studies and on present findings, it seems that dopamine is stored in the strongly fluorescent cells after injection of L-dopa, and that a compound closely related to 5-hydroxytryptophan. A non-specific, granular fluorescence appeared after 5-hydroxytryptophan and, to a lesser extend, L-dopa treatment. It probably represents autofluorescence of lysosomes, which are numerous in these circumstances.
D006916:5-Hydroxytryptophan / Q000494:pharmacology*; D000818:Animals; D005557:Formaldehyde / Q000494:pharmacology; D006651:Histocytochemistry; D007980:Levodopa / Q000494:pharmacology*; D008297:Male; D008856:Microscopy, Fluorescence; D010903:Pituitary Gland, Anterior / Q000032:analysis* / Q000187:drug effects; D011784:Quail / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D005557:Formaldehyde; D007980:Levodopa; D006916:5-Hydroxytryptophan