Specific recognition of the product of a transferred major histocompatibility complex gene by cytotoxic T lymphocytes.
Mouse L cells transfected with a genomic clone containing the H-2Ld gene (8-5 cells) were shown to function as targets for H-2Ld-specific cytotoxic T lymphocytes (CTL). The CTL-mediated lysis of 8-5 cells was shown to be H-2Ld specific by the use of (i) CTL with restricted reactivity, (ii) unlabeled target inhibiton, and (iii) monoclonal antibody inhibiton. We also demonstrated that 8-5 cells could function as targets for antibody-plus-complement-mediated cell lysis. Specificity was confirmed by using H-2Ld-specific monoclonal antibodies. These experiments demonstrate that the gene products of a major histocompatibility complex genomic clone can be functionally expressed in a foreign cell and can mediate immunologically specific cellular interactions.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D003167:Complement Activation; D003602:Cytotoxicity, Immunologic; D005786:Gene Expression Regulation; D005796:Genes; D005818:Genetic Engineering; D006183:H-2 Antigens / Q000235:genetics*; D007518:Isoantibodies; D008285:Major Histocompatibility Complex; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D006183:H-2 Antigens; D007518:Isoantibodies