Role of lymphotoxin in antibody-dependent cell-mediated cytotoxicity (ADCC).
The precise mechanism by which human peripheral blood lymphocytes (PBL) lyse antibody-coated target cells in antibody-dependent cell-mediated cytotoxicity (ADCC) is unclear. Antiserum against purified human alpha-lymphotoxin (anti-LT serum) inhibited an ADCC reaction with human PBL as effector cells and antibody-coated mouse P815 mastocytoma cells as targets. This observation suggests that alpha-lymphotoxin is instrumental in the destruction of antibody-coated targets. The inhibition was not due to a cytotoxic effect of the anti-LT serum on the PBL, nor was it caused by the presence of IgG aggregates. PBL incubated with cycloheximide were still cytotoxic in the ADCC system, although to a somewhat lower degree. This finding suggests that de novo protein synthesis not essential in the cytotoxic reaction.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D003513:Cycloheximide / Q000494:pharmacology; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007136:Immunoglobulins; D008233:Lymphotoxin-alpha / Q000276:immunology*; D051379:Mice; D011817:Rabbits
D007106:Immune Sera; D007136:Immunoglobulins; D008233:Lymphotoxin-alpha; D003513:Cycloheximide