Intracellular measurement of Na activity using neutral carrier Na ion-selective microelectrode.
To measure intracellular Na+ activities, a double-barreled Na ion-selective microelectrode was constructed with a neutral carrier Na+ ligand (ETH 227). The slope constant and detection limit were 56 mV and 3-5 mM, respectively, the selectivity of Na+/K+ being 40-50. In the bullfrog, the cell Na+ was 14.4 mEq/liter for the proximal tubule and 12.6 mEq/liter for the sartorius muscle.
D000818:Animals; D007687:Kidney Tubules, Proximal / Q000502:physiology*; D008024:Ligands; D008564:Membrane Potentials; D008839:Microelectrodes; D009132:Muscles / Q000502:physiology*; D011892:Rana catesbeiana; D012964:Sodium / Q000502:physiology*
D008024:Ligands; D012964:Sodium