Production of Con A-induced helper T cell replacing factor requires a T cell and an Ia-positive non-T cells.
Evidence is presented that indicates that two cells are required for the production of Con A-induced, helper T cell replacing factor. The factor production involves both a T cell and an IA,IE/C-positive non-T cell. The T cell and the Ia-positive cell need not be H-2 compatible. Limiting dilution analyses suggested that the Ia-positive cell is limiting in normal spleen. A hypothesis is put forward that the activation of T cells by Con A may involve "gluing" normally specific, functional T cells to the Ia expressing cells with which they would normally interact in antigen-specific activation.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002448:Cell Adhesion; D002450:Cell Communication; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D006183:H-2 Antigens; D007106:Immune Sera / Q000494:pharmacology; D008222:Lymphokines / Q000302:isolation & purification*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008819:Mice, Nude; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013934:Thymectomy
D000961:Antilymphocyte Serum; D006183:H-2 Antigens; D007106:Immune Sera; D008222:Lymphokines; D003208:Concanavalin A