Studies on human epidermal Langerhans cells. I. Allo-activating and antigen-presenting capacity.
Human epidermis was separated from dermis by means of a suction blister device and dissociated with trypsin. The epidermal cell suspensions obtained contained 3--5% Langerhans cells as judged by immunofluorescence staining ot the cells with a rabbit anti-DR antiserum. The epidermal cells were co-cultured with purified allogeneic T cells and with autologous T cells with or without PPD of tuberculin. A strong T-cell response to allogeneic epidermal cells was obtained, as was a strong T-cell response to PPD, provided autologous epidermal cells were also present. Pre-treatment of the epidermal cells with anti-DR antiserum plus complement abolished both these responses. These data indicate that epidermal cells are able to substitute for macrophages both in the allo-activating and in the antigen-presenting function. Since the responsible cells were DR-positive, it is highly probable that the cells responsible for these functions are the Langerhans cells.
D001323:Autoantibodies / Q000276:immunology; D001324:Autoantigens / Q000276:immunology; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D007518:Isoantibodies / Q000276:immunology; D007519:Isoantigens / Q000276:immunology; D007801:Langerhans Cells / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D001323:Autoantibodies; D001324:Autoantigens; D007518:Isoantibodies; D007519:Isoantigens