Correlation of cell cycle parameters with radiation sensitivity in a series of murine L5178Y cells.
The X-ray dose--response parameters (Do, n and Dq) of 10 strains of murine L5178Y cells spanning a wide range of radiation sensitivities were characterized. The proportions of cells residing in G1, S and G2 + M phases of the cell cycle in exponentially growing cell populations were estimated using DNA microfluorometric techniques. The radiosensitive strains contained a lower proportion of cells in G1 phase and a somewhat larger proporation of cells in S phase than did the radioresistant strains, reflecting a direct correlation between radioresistance and the duration of G1 phase (and possibly an inverse correlation with the duration of the S phase) in these closely related cell strains. These results are discussed in terms of the putative role of G1 in governing radiosensitivity of cells and tumours.
D000818:Animals; D002453:Cell Cycle / Q000528:radiation effects*; D002460:Cell Line; D002470:Cell Survival; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D007399:Interphase / Q000528:radiation effects; D008223:Lymphoma / Q000473:pathology; D051379:Mice; D008938:Mitosis / Q000528:radiation effects; D009374:Neoplasms, Experimental / Q000473:pathology*; D011836:Radiation Tolerance; D013997:Time Factors; D014965:X-Rays