A micromethod for the detection of Tg and Tm cells. Technical aspects for the enumeration of human T-cell subpopulations.
A rosette technique for the detection of human T-lymphocyte subpopulations with Fc receptors for IgG (TG cells) or IgM (TM cells) has been worked out using minimal amounts of IgG and IgM antibodies. Antibody-coated ox red blood cells (EA) were used as indicator cells. Optimal conditions for rosette enumerations required intimate T-cell-EA contact and incubation of cell mixtures for 3 h at 4 degrees C. The TM-cell level increased after culture of T cells, and TM-cell rosettes were found to be more fragile. The rosette assay was found to be reproducible and thus useful in clinical immunology.
D000328:Adult; D000818:Animals; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012397:Rosette Formation; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013696:Temperature
D007074:Immunoglobulin G; D007075:Immunoglobulin M