The failure of nonresponder mice to develop IgG memory assessed by in vitro culture with an antigen-LPS conjugate.
A hen egg-white lysozyme-lipopolysaccharide complex (HEL-LPS) can stimulate an in vitro IgG response, but only from HEL-primed B lymphocytes; unprimed cells only produce an IgM response. These conditions were used to determine whether IgG memory B cells are cryptically induced in B10 nonresponder (H-2b) mice after an HEL injection protocol. The usual i.p. immunization that triggers IgG memory production in congenic responder strain mice fails to yield IgG in vitro from HEL-primed B10 spleen cells after stimulation with HEL-LPS. However, injection protocols immunogenic for B10 mice do engender IgG-memory cells. These results imply that the T helper cell population necessary for triggering B cells to the IgG memory stage cannot develop in the nonresponder mouse, presumably due to HEL-specific T suppressor cells.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005620:Freund's Adjuvant / Q000494:pharmacology; D006183:H-2 Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007156:Immunologic Memory; D007700:Kinetics; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009113:Muramidase / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology
D006183:H-2 Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D010047:Ovalbumin; D005620:Freund's Adjuvant; D009113:Muramidase