Results of endoneural injection of Guillain-Barré serum in Lewis rats.
We previously studied the neurophysiologic effect of endoneural injection of serum from patients with acute inflammatory-demyelinating polyradiculoneuropathy into the sciatic nerve of Sprague-Dawley rats and did not observe a statistically significant difference between the results with that serum and control serum at 1 week. Because of potential strain susceptibility to acute inflammatory-demyelinating polyradiculoneuropathy serum after endoneural injection, the syngeneic Lewis rat--which is more susceptible than other strains of rats to experimental allergic neuritis (considered to be an experimental model of acute inflammatory-demyelinating polyradiculoneuropathy)--was studied. We used sera from five severely affected patients and also used a more sensitive (compared with our earlier studies) electrophysiologic approach that included in vivo monophasic compound action potential recordings and paired pulses. No statistically significant differences in conduction velocity, amplitude, indices of dispersion, or time-integral percentage were found between disease and control sera at 1 week. We conclude that the human acute inflammatory-demyelinating polyradiculoneuropathy serum tested did not contain measurable demyelinating activity for rat nerve in excess of that of control serum.
D000368:Aged; D000818:Animals; D004594:Electrophysiology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009444:Neuritis, Autoimmune, Experimental / Q000209:etiology* / Q000503:physiopathology; D011129:Polyradiculoneuropathy / Q000276:immunology* / Q000503:physiopathology; D051381:Rats; D011917:Rats, Inbred Lew; D012584:Sciatic Nerve / Q000503:physiopathology