Detection of N-hydroxy-2-acetylaminofluorene-DNA adducts in rat liver measured by radioimmunoassay.
Antibodies to imidazole ring opened derivatives of alkali treated N-(guanosin-8-yl)-2-aminofluorene (1-[6-(2,5-diamino-4-oxopyrimidinyl-N6-riboside)]-3-(2-fluorenyl)urea were elicited in rabbits by immunization with a conjugate between the ring-opened derivative and bovine serum albumin. The specificity of the antibodies was studied by radioimmunoassay. These antibodies and antibodies to N-(guanosin-8-yl)-N-acetyl-2-aminofluorene were used to titrate the adducts formed in liver DNA of rats treated with N-hydroxy-2-acetylaminofluorene. The ring-opened derivatives represent approximately 10% of the dGuo-C8-adducts.
D015073:2-Acetylaminofluorene / Q000031:analogs & derivatives* / Q000032:analysis; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D004247:DNA / Q000378:metabolism*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D006881:Hydroxyacetylaminofluorene / Q000008:administration & dosage / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D008099:Liver / Q000378:metabolism*; D008297:Male; D008722:Methods; D011863:Radioimmunoassay; D051381:Rats; D011917:Rats, Inbred Lew
C018776:N-(deoxyguanosin-8-yl)acetylaminofluorene; D006881:Hydroxyacetylaminofluorene; D004247:DNA; D015073:2-Acetylaminofluorene; D003849:Deoxyguanosine