Radiosensitivity and postirradiation changes of bone marrow clonogenic stromal mechanocytes.
The in vitro colony assay was used to determine radiosensitivity and post-irradiation changes of guinea pig clonogenic bone marrow stromal fibroblasts (CFU-F). D0 of the CFU-F survival curve proved to be about 200 rad, while n is 1.4. Immediately after total-body exposure to 400 rad and to local exposure of tibias to 400 rad, the number of CFU-F diminishes in accordance with D0; it sharply increases after 6 hours and then decreases again. In the period between the eighth and twelfth day following local irradiation, the number of CFU-F doubled in the course of 30-35 hours but later the rate of recovery slowed down. Practically no CFU-F were traced during 3 months after local exposure to 2 krad.
D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects; D003037:Cobalt Radioisotopes; D003114:Colony-Forming Units Assay; D005347:Fibroblasts / Q000528:radiation effects; D005720:Gamma Rays; D006168:Guinea Pigs; D066298:In Vitro Techniques; D011836:Radiation Tolerance; D014965:X-Rays
D003037:Cobalt Radioisotopes