title: The effect of antibodies against Escherichia coli small ribosomal subunit proteins on protein synthesis by rat liver ribosomes.
abstract: Monovalent Fab fragments from immunoglobulins, directed against Escherichia coli small ribosomal subunit proteins, were tested for their effect on poly(U)-directed synthesis of polyphenylalanine by rat liver ribosomes. Of the 18 Fabs tested, 3, anti-S10, anti-S12, and anti-S14, inhibited polyphenylalanine synthesis; antibodies against 3 of the 21 E. coli ribosomal proteins (S1, S16, and S17) were not tested. The others were either without any appreciable effect or, as in the case of anti-S11 and anti-S18, inhibited far less. Antibodies against S12 and S14 (but not against S10) inhibited elongation factor 1-dependent binding of [14C]Phe-tRNA to rat liver 80 S ribosomes. The presumption is that the prokaryotic (E. coli) ribosomal proteins, S10, S12, and S14, possess antigenic determinants also present in eukaryotic (rat liver) ribosomal proteins; the rat liver proteins have not yet been identified.
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000378:metabolism*; D007140:Immunoglobulin Fab Fragments; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D010445:Peptide Elongation Factors / Q000378:metabolism; D010649:Phenylalanine; D014176:Protein Biosynthesis; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D051381:Rats; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D007140:Immunoglobulin Fab Fragments; D010445:Peptide Elongation Factors; D012346:RNA, Transfer, Amino Acyl; D010649:Phenylalanine