Insulin receptor binding and degradation in IM-9 cultured human lymphocytes - importance of extracellular degradation.
Insulin degradation in IM-9 cultured human lymphocytes occurs extracellularly. In the presence of 0.1% bovine serum albumin, insulin degradation products are formed that are soluble in 5% trichloroacetic acid. In the presence of 5% bovine serum albumin, the major products of insulin degradation are insoluble in 5% trichloroacetic acid and have little or no biological activity. Inhibition of binding with Concanavalin A or antireceptor antibody does not affect the rate of insulin degradation.
D000906:Antibodies; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism*; D011972:Receptor, Insulin / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D007328:Insulin; D003208:Concanavalin A; D011972:Receptor, Insulin