Serological cross-reactivity of murine allo-anti-Ias antibody with the molecule on human antigen-presenting cells.
Murine allo-anti-IaS (A.TL anti-A.TH) antibody cross-reacted with human B-cells and antigen-presenting cells, while the antibody did not cross-react with human T-cells, to be precise, PPD-specific proliferative T-cells. Furthermore, the murine allo-anti-IaS antibody could inhibit the PPD-specific responses of human PBL. Thus, the murine allo-anti-IaS antibody affixes on the Ia-like molecule of human antigen-presenting cells in PPD-specific responses. A genetic mapping study of the serological cross-reactive antibody, by absorbing with the spleen cells from B10.S(9R), A.TH, A.TL and SJL mice in order to determine the specificities for the I subregions of the H-2, revealed that the serological cross-reactivity with human PBL was caused by the anti-I-AS antibody.
D000818:Animals; D001667:Binding, Competitive; D003165:Complement System Proteins / Q000378:metabolism; D003429:Cross Reactions; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D011946:Receptors, Antigen; D014373:Tuberculin / Q000276:immunology
D000949:Histocompatibility Antigens Class II; D007518:Isoantibodies; D011946:Receptors, Antigen; D014373:Tuberculin; D003165:Complement System Proteins