The biochemical genetics of TL antigens on mouse thymocytes.
Molecules bearing thymus leukemia (TL) alloantigen were isolated by immunoprecipitation from detergent-solubilized thymocyte lysates. Antisera used included monoclonal antibodies (anti-TL.m1, anti-TL.m2, anti-TL.m3), monospecific anti-TL.5 alloantisera and multispecific anti-TL.1,2,3,5 antiserum. Apparently, each of these reagents immunoprecipitates the same single 45,000 molecular weight Tla gene product as shown by identity on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focusing and by sequential precipitation studies. Allelic TL molecules, coded by the Tlaa and Tlad genes, were shown to be distinguishable by SDS-PAGE, and tryptic peptide mapping experiments. Both allelic TL molecules could be isolated from thymocytes of (Tlaa x Tlad)F1 mice. These results suggest that, at least for the Tlaa-Tlad allelic differences, the polymorphism and antigenicity of TL is determined by variation in amino acid composition.
D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera / Q000145:classification / Q000494:pharmacology; D007525:Isoelectric Focusing; D008562:Membrane Glycoproteins; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D010455:Peptides; D011110:Polymorphism, Genetic; D014176:Protein Biosynthesis; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D000951:Antigens, Neoplasm; D007106:Immune Sera; D008562:Membrane Glycoproteins; D010455:Peptides; C028388:thymus-leukemia antigens