Anti-self receptors. IV. H-2-restricted receptors on thymocytes recognize carbohydrate structures on target cells.
Rosetting between thymocytes and autologous erythrocytes in mediated by receptors on thymocytes that primarily recognize self H-2L molecules on erythrocytes. This paper describes preliminary attempts to chemically characterize the receptor and acceptor molecules involved in this H-2-restricted interaction. On the basis of sugar inhibition studies and the sensitivity of the receptors to protease and glycosidase treatments it appears that a protein receptor on thymocytes recognizes the carbohydrate portion of a glycoprotein on erythrocytes. Furthermore, the thymocyte receptor appears to recognize terminal D-galactose, D-mannose and sialic acid residues on a branched-chain carbohydrate structure on erythrocytes, with mouse strains of different H-2 haplotype expressing carbohydrate structures that differ in the linkage of these three terminal sugars. These findings indicate that H-2-restricted carbohydrate-protein interactions can occur between cells, a conclusion with important theoretical implications.
D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology*; D004912:Erythrocytes / Q000201:enzymology / Q000276:immunology; D005688:Galactosamine / Q000494:pharmacology; D006183:H-2 Antigens / Q000276:immunology*; D008358:Mannose / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D011887:Raffinose / Q000494:pharmacology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D012397:Rosette Formation; D012794:Sialic Acids / Q000494:pharmacology; D013601:T-Lymphocytes / Q000201:enzymology
D002241:Carbohydrates; D006183:H-2 Antigens; D011948:Receptors, Antigen, T-Cell; D012794:Sialic Acids; D005688:Galactosamine; D011887:Raffinose; D008358:Mannose