Preparation of colony stimulating activity from human leukemic urine: hydrophobic chromatography on propylamine-agarose.
Urinary colony stimulating factor (CSF) from human leukemic urine is retained on propylamine-agarose hydrophobic chromatography; about 70% of total activity is eluted by the addition of 1 M NaCl with significant increase in specific activity. Incomplete binding of urinary CSF to this resin further confirm that biological activity is a heterogeneous pool of different molecular species. Analysis of the macro- and microscopic composition of colonies indicates that CSF resulting from this purification step maintains the ability to stimulate both granulocytic and macrophage progenitors.
D002847:Chromatography, Agarose / Q000379:methods; D003115:Colony-Stimulating Factors / Q000302:isolation & purification* / Q000652:urine; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D007951:Leukemia, Myeloid / Q000652:urine*; D015470:Leukemia, Myeloid, Acute / Q000652:urine*; D008264:Macrophages / Q000166:cytology; D011437:Propylamines
D003115:Colony-Stimulating Factors; D011437:Propylamines