Nonrandom distribution of repeated DNA sequences with respect to supercoiled loops and the nuclear matrix.
The DNA in a eukaryotic nucleus is arranged into a series of supercoiled loops that are anchored at their bases to the nuclear matrix. We have analyzed the DNA sequences that are closest to the matrix attachment points for their relative content of specific repeated sequences. Sequences were enriched (mouse satellite, human Alu family) or depleted (mouse EcoRI repeat, monkey alpha component), depending on the specific sequence and species examined. These results can be understood in terms of a nonrandom arrangement of DNA sequences with respect to nuclear DNA loops.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis* / Q000648:ultrastructure; D002522:Chlorocebus aethiops; D004247:DNA / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics; D004278:DNA, Superhelical / Q000235:genetics*; D006801:Humans; D051379:Mice; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D004276:DNA, Satellite; D004278:DNA, Superhelical; D004247:DNA