Inhibition of proliferation of a murine myeloma cell line and mitogen-stimulated B lymphocytes by the antibiotic amphotericin B (Fungizone).
Amphotericin B at the concentration normally used for routine suppression of fungal infection in tissue culture strongly inhibits the proliferation of NS1/1 myeloma cells and the LPS-induced activation of B lymphocytes from mouse spleen. The proliferation of T lymphocytes induced by concanavalin A (Con A) was less affected by the antibiotic, indicating that B-lymphocyte proliferation was preferentially inhibited. The unexpected sensitivity of B-lymphoid cells to amphotericin B precludes its use as an anti-fungal agent in the production of hybridomas from fusions between these cells.
D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002455:Cell Division / Q000187:drug effects; D002459:Cell Fusion; D002460:Cell Line; D002999:Clone Cells / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000276:immunology / Q000473:pathology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D000666:Amphotericin B