title: Platelet microtubule subunit proteins.
abstract: Blood platelets contain microtubule proteins (tubulin and HMWs) which can be polymerised "in vitro" to form structure which resemble the microtubules seen in the intact platelet. Platelet tubulin is composed of two non-identical subunits alpha and beta tubulin which have molecular weights around 55,000 but can be resolved in alkaline SDS-polyacrylamide gel electrophoresis. These subunits associate as dimers with sedimentation coefficients of about 5.7 S although it is not known whether the dimer protein is a homo- or hetero-dimer. The dimer tubulin binds the anti-mitotic drug colchicine and the kinetics of this binding are similar to those reported for neurotubulins. Platelet microtubules also contain two HMW proteins which appear to be essential and integral components of the fully assembled microtubule. These proteins have molecular weights greater than 200,000 daltons. Fluorescent labelled antibodies to platelet and brain tubulins stain long filamentous microtubular structures in bovine lens epithelial cells and this pattern of staining is prevented by exposing the cells to conditions known to cause depolymerization of cell microtubules.
mesh_terms: D000818:Animals; D001665:Binding Sites; D001792:Blood Platelets / Q000032:analysis*; D001798:Blood Proteins / Q000302:isolation & purification*; D002417:Cattle; D002499:Centrifugation, Density Gradient; D003078:Colchicine / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D008870:Microtubules / Q000032:analysis* / Q000648:ultrastructure; D008970:Molecular Weight; D011817:Rabbits; D013552:Swine; D014404:Tubulin / Q000302:isolation & purification
chemical_list: D001798:Blood Proteins; D007106:Immune Sera; D014404:Tubulin; D003078:Colchicine