Cryoprotection of human bone marrow committed stem cells (CFU-c) by dextran, glycerol and dimethyl sulfoxide.
Dextran, glycerol and dimethyl sulfoxide (DMSO), alone or in combination, were used for cryoprotection of human bone marrow cells. The viability of cryopreserved cells was assessed by culture of myelopoiesis-committed stem cells (CFU-c) in vitro. A significantly better protection against freezing injury was obtained by 9% dextran in combination with 3 or 5% DMSO, and also with 5 or 10% DMSO alone, than with either 15% glycerol or 9% dextran with 1% DMSO.
D000818:Animals; D001141:Aromatase / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004967:Estrogens / Q000096:biosynthesis*; D006801:Humans; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D009338:Necturus maculosus; D013254:Steroid 17-alpha-Hydroxylase / Q000378:metabolism; D013329:Structure-Activity Relationship; D013737:Testis / Q000378:metabolism* / Q000648:ultrastructure
D004967:Estrogens; D003577:Cytochrome P-450 Enzyme System; D001141:Aromatase; D013254:Steroid 17-alpha-Hydroxylase