Early appearance of abnormally migrating serum IgM in control and cyclophosphamide-treated NZB/NZW mice.
An augmented incidence of neoplasms was observed in autoimmune NZB/NZW mice receiving longterm immunosuppressive therapy with cyclophosphamide. This observation afforded a unique opportunity to test for paraproteins in 58 serial samples of serum from 39 mice dying with tumors or with renal disease/vasculitis. In 6 animals, abnormally migrating monoclonal IgM precipitin lines appeared at 40--56 weeks of age and persisted until death. In 3 animals, paraproteins appeared 32 to 43 weeks before reticuloendothelial neoplasms were observed. Three mice with abnormally migrating IgM died with renal disease/vasculitis. Abnormal IgM did not correlate with histologic changes of Sjögren's syndrome, lymphadenopathy, or activity of autoimmune disease. In 1 instance, an IgM paraprotein appeared and persisted in a mouse receiving prolonged treatment with a high dose of cyclophosphamide.
D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology; D003520:Cyclophosphamide / Q000494:pharmacology*; D005260:Female; D007122:Immunoelectrophoresis; D007075:Immunoglobulin M / Q000097:blood*; D007674:Kidney Diseases / Q000139:chemically induced / Q000401:mortality; D008297:Male; D051379:Mice; D008814:Mice, Inbred NZB; D008815:Mice, Inbred Strains; D009369:Neoplasms / Q000139:chemically induced / Q000401:mortality; D013997:Time Factors; D014657:Vasculitis / Q000139:chemically induced / Q000401:mortality
D000974:Antibodies, Antinuclear; D007075:Immunoglobulin M; D003520:Cyclophosphamide