A comparison of tetrahydrofolate and 5-formyltetrahydrofolate in correcting the impairment of thymidine synthesis in pernicious anaemia.
5-formyltetrahydrofolate and tetrahydrofolate were added to marrow cells from patients with untreated pernicious anaemia at 1, 5 and 50 nmol doses in the deoxyuridine suppression test. At all 3 dose levels formyltetrahydrofolate was significantly more effective in correcting the defect of thymidine synthesis in pernicious anaemia, than tetrahydrofolate. The data suggest that formylation of tetrahydrofolate is necessary for its normal utilization.
D000752:Anemia, Pernicious / Q000097:blood / Q000188:drug therapy*; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D002955:Leucovorin / Q000627:therapeutic use*; D013763:Tetrahydrofolates / Q000627:therapeutic use*; D013936:Thymidine / Q000096:biosynthesis
D013763:Tetrahydrofolates; C030371:5,6,7,8-tetrahydrofolic acid; D002955:Leucovorin; D013936:Thymidine