title: Enzyme-linked immunosorbent assay (ELISA) for detecting IgM and IgE antibodies in human schistosomiasis.
abstract: Sera from patients with acute or early and chronic schistosomiasis were examined for IgG, IgM, and IgE antibody by an enzyme-linked immunosorbent assay technique, using soluble egg antigen from Schistosoma mansoni. Cercarial/adult IgG antibody ratios were determined, using soluble cercarial and adult worm antigens. Sera with cercarial/adult ratios indicative of acute or early schistosomiasis also contained specific IgM antibodies. Schistosome IgE antibody was found in sera from patients with acute schistosomiasis, but in only 1 of 10 sera from patients with chronic schistosomiasis. The inability of ELISA to detect IgE antibodies in chronic sera indicates that it may be a relatively insensitive measure of IgE antibodies in those patients with chronic schistosomiasis.
mesh_terms: D000906:Antibodies / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis*; D012550:Schistosoma mansoni / Q000276:immunology; D012552:Schistosomiasis / Q000276:immunology*
chemical_list: D000906:Antibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007073:Immunoglobulin E