Interleukin 2 secretion by sensitized cells from mice infected with Nematospiroides dubius: kinetics and specificity of the response.
The lymphokine Interleukin 2 (IL2) is secreted by T lymphocytes from mice infected by the murine parasitic nematode Nematospiroides dubius upon in vitro re-stimulation by specific parasite antigens. This study showed that the lymphocytes secreting IL2 were located in the lymphoid tissues draining the site of infection as well as in the spleen and peritoneal cavity of infected mice. There were marked fluctuations in the numbers of IL2 secreting cells in the posterior gastric lymph nodes and the peritoneal cavity at various times following single or multiple infections. However, there was no correlation between the appearance of IL2 secreting cells in infected mice and the development of resistance to re-infection by third stage larvae (L3). The specificity of the IL2 response for N. dubius antigens was demonstrated by the finding that antigen preparations from other helminths did not stimulate a response. It was also found that living but not killed N. dubius L3 could stimulate IL2 secretion, emphasizing the importance of living L3 in the induction of resistance to re-infection.
D000818:Animals; D000941:Antigens / Q000276:immunology; D001202:Ascitic Fluid / Q000166:cytology; D005210:Fasciola hepatica / Q000276:immunology; D006189:Haemonchus / Q000276:immunology; D007376:Interleukin-2 / Q000378:metabolism*; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D009348:Nematoda / Q000276:immunology*; D009349:Nematode Infections / Q000276:immunology*; D013045:Species Specificity; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D000941:Antigens; D007376:Interleukin-2