title: Biosynthesis and catabolism of 6-deoxy L-talitol by Klebsiella aerogenes mutants.
abstract: A mutant strain of Klebsiella aerogenes was constructed and, when incubated anaerobically with L-fucose and glycerol, synthesized and excreted a novel methyl pentitol, 6-deoxy L-talitol. The mutant was constitutive for the synthesis of L-fucose isomerase but unable to synthesize L-fuculokinase activity. Thus, it could convert the L-fucose to L-fuculose but was incapable of phosphorylating L-fuculose to L-fuculose 1-phosphate. The mutant was also constitutive for the synthesis of ribitol dehydrogenase, and in the presence of sufficient reducing power this latter enzyme catalyzed the reduction of the L-fuculose to 6-deoxy L-talitol. The reducing equivalents required for this reaction were generated by the oxidation of glycerol to dihydroxyacetone with an anaerobic glycerol dehydrogenase. The parent strain of K. aerogenes was unable to utilize the purified 6-deoxy L-talitol as a sole source of carbon and energy for growth; however, mutant could be isolated which had gained this ability. Such mutants were found to be constitutive for the synthesis of ribitol dehydrogenase and were thus capable of oxidizing 6-deoxy L-talitol to L-fuculose. Further metabolism of L-fuculose was shown by mutant analysis to be mediated by the enzymes of the L-fucose catabolic pathway.
mesh_terms: D005643:Fucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000378:metabolism*; D009154:Mutation; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism; D013402:Sugar Alcohols / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: C024252:6-deoxy L-talitol; D013402:Sugar Alcohols; D005643:Fucose; D013401:Sugar Alcohol Dehydrogenases; D005990:Glycerol