[Effect of pH on photoinduced electron transport in reaction center preparations from Rhodopseudomonas sphaeroides].
The effect of ph on kinetics of photo-induced electron transport process was studied on reaction centre preparations extracted by LDAO treatment of R. sphaeroides chromatophores (strain 1760-1). Reaction centre activity was found to be optimal at pH 7--9. Within this range of pH, the rate constant, KX1X2, for electron transfer from the primary FeQ acceptor complex (X1) to the secondary quinone acceptor X2 appeared to be invariant. On pH lowering from 7 to 5 the value of KX1X2 increases by several times. It abruptly drops, when pH is increased from 9 to 9.5. The nature of these phenomena including influence of pH on KX1X2 value is discussed.
D001665:Binding Sites; D004579:Electron Transport / Q000528:radiation effects; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008027:Light; D010788:Photosynthesis; D012242:Rhodobacter sphaeroides / Q000378:metabolism*
Vliianie pH na fotoindutsirovannyĭ perenos élektrona v preparatakh reaktsionnykh tsentrov iz Rhodopseudomonas sphaeroides.