Vertebrate cells express protozoan antigen after hybridization.
Epimastigotes, the invertebrate host stage of Trypanosoma cruzi, the protozoan parasite causing Chagas' disease in man, were fused with vertebrate cells by using polyethylene glycol. Hybrid cells were selected on the basis of T. cruzi DNA complementation of biochemical deficiencies in the vertebrate cells. Some clones of the hybrid cells expressed T. cruzi-specific antigen. It might be possible to use selected antigens obtained from the hybrids as vaccines for immunodiagnosis or for elucidation of the pathogenesis of Chagas' disease.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification; D002459:Cell Fusion; D002460:Cell Line; D002999:Clone Cells; D006822:Hybrid Cells / Q000276:immunology*; D006824:Hybridization, Genetic; D008322:Mammals; D011092:Polyethylene Glycols; D014349:Trypanosoma cruzi / Q000235:genetics / Q000276:immunology*
D000941:Antigens; D011092:Polyethylene Glycols