Conversion of monocytes to cells capable of anchorage-independent growth in vitro.
We investigated the time-course involved in the conversion of mouse blood monocytes in vitro in cells capable of anchorage-independent growth. Two criteria were used to define when monocytes were fully converted to cells similar to mononuclear phagocytes present in inflammatory exudate, such as thioglycollate medium (TM)-elicited peritoneal exudate. They were the production of high levels of plasminogen activators and an ability to undergo anchorage-independent growth. Resident peritoneal macrophages were used as controls and for comparison. Our studies indicated that monocytes, but not resident peritoneal macrophages, could be converted to cells similar to TM-elicited mononuclear phagocytes after 2 d in culture.
D000362:Agar; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D005122:Exudates and Transudates; D005260:Female; D005342:Fibrinolysis; D005780:Gelatin / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology; D008297:Male; D051379:Mice; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*
D003115:Colony-Stimulating Factors; D005780:Gelatin; D000362:Agar