Study of the effect of single or multiple doses of BCG on antibody-dependent cellular cytotoxicity.
The in vivo effects of BCG were studied on antibody-dependent cellular cytotoxicity (ADCC) using an assay in which the effectors were spleen cells of BCG-treated mice, and the targets antibody-coated chicken red blood cells (CRBC). In the study of the time course of action, an increased ADCC-activity was observed starting at day 10 with a maximum (four-fold augmentation) at day 14 after BCG treatment. The nature of the effector cell was investigated: in normal mice the activity was found in two cell populations, i.e. macrophages and theta-negative (theta-) nylon-adherent spleen cells. A BCG treatment increased the ADCC activity of macrophages of theta- nylon-adherent spleen cells and induced a population of theta- nylon-non-adherent spleen cells. In old mice, the effects of BCG were compared after a weekly long-term (6 months) treatment and after administration of a single dose. It was concluded that ADCC is higher in old mice than in young mice and can be further increased by treatment with a single dose of BCG in contrast with the long-term treatment which impaired this immune reaction.
D000375:Aging; D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D001500:BCG Vaccine / Q000008:administration & dosage* / Q000276:immunology; D002448:Cell Adhesion; D002469:Cell Separation; D004306:Dose-Response Relationship, Immunologic; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D007700:Kinetics; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D001500:BCG Vaccine