T-lymphocyte heterogeneity: wheat germ agglutinin labeling of transmembrane glycoproteins.
We have recently described "fracture-label" techniques that permit direct cytochemical labeling of freeze-fractured cells. We report here the use of fracture-labeling to investigate the distribution and partition of wheat germ agglutinin (WGA) receptor sites over the protoplasmic and exoplasmic plasma membrane faces of freeze-fractured human thymus-derived (T) lymphocytes. All exoplasmic faces are strongly labeled by WGA. In contrast, the protoplasmic faces exhibit remarkable variation, ranging from virtual absence of label in some faces to very high densities in other faces. We interpret the presence of WGA receptor sites over the protoplasmic faces to reflect the presence of transmembrane WGA-binding sialoglycoproteins that, during freeze-fracture, partition with the inner half of the plasma membrane. Our results, therefore, indicate heterogeneous expression of integral membrane proteins within populations of human T cells. Fracture-label techniques thus represent an additional tool in the definition of lymphocyte subpopulations.
D001402:B-Lymphocytes / Q000276:immunology / Q000648:ultrastructure; D005614:Freeze Fracturing; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D037102:Lectins; D008565:Membrane Proteins / Q000276:immunology*; D008854:Microscopy, Electron; D011975:Receptors, Mitogen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D014909:Wheat Germ Agglutinins
D006023:Glycoproteins; D037102:Lectins; D008565:Membrane Proteins; D011975:Receptors, Mitogen; D014909:Wheat Germ Agglutinins; C027503:wheat germ agglutinin receptor