title: Alterations in the cell envelope of Escherichia coli late in bacteriophage T4 infection.
abstract: The cell envelope of Escherichia coli was examined for changes during late stages of bacteriophage T4 infection. Late events in T4 infection are shown to result in (i) a reduction in the effectiveness of membrane separation procedures employing either isopycnic sucrose gradient centrifugation or selective solubilization of inner membrane by detergent (Sarkosyl or Triton X-100), (ii) the appearance of a 54 000 dalton host protein in membrane preparations, (iii) the adventitious presence of detergent-resistant phage morphogenetic structures in membrane preparation, and (iv) a decrease in the activity of NADH oxidase and an apparent alteration in its association with inner membrane. These modifications occur regardless of the state of the e and t genes of T4.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis; D002458:Cell Fractionation; D002462:Cell Membrane / Q000502:physiology* / Q000648:ultrastructure; D004926:Escherichia coli / Q000201:enzymology / Q000502:physiology* / Q000648:ultrastructure; D005814:Genes, Viral; D008565:Membrane Proteins / Q000096:biosynthesis; D008970:Molecular Weight; D009245:NADH Dehydrogenase / Q000378:metabolism; D013604:T-Phages / Q000201:enzymology / Q000502:physiology*; D014779:Virus Replication
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D009245:NADH Dehydrogenase