title: Transcription of Friend virus proviral sequences in isolated nuclei.
abstract: Transcription studies using isolated Friend nuclei and Escherichia coli polymerase are presented. Combination of the techniques of thiol-Sepharose chromatography and cDNA-Sepharose hybridisation has resulted in a system in which the transcription of the Friend virus proviral sequences with endogenous and E. coli polymerase can be examined. The results show that the percentage of Friend viral-specific sequences in RNA transcribed by E. coli RNA polymerase and by endogenous RNA polymerase in isolated nuclei are similar. The percentage of viral-specific sequences synthesized in isolated nuclei is similar to that found in Friend cell nuclear RNA.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D005622:Friend murine leukemia virus / Q000378:metabolism*; D007700:Kinetics; D007942:Leukemia, Experimental; D051379:Mice; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000096:biosynthesis*; D014158:Transcription, Genetic
chemical_list: D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases