Histochemical determination of estrogen and progesterone binding in fine needle aspirates of breast cancer. Correlation with conventional biochemical assays.
Estrogen and progesterone binding sites were assayed in patients with primary, recurrent and metastatic mammary carcinoma using a new histochemical technique. This histochemical assay employed fluorescein-labeled conjugates of bovine serum albumin covalently bound to estradiol or progesterone. In a double-blind study in which the results of histochemistry were correlated with those obtained by a conventional biochemical assay, there was 83% correlation for estrogen binding in 41 cases and 87% correlation for progesterone binding in 31 cases.
D001665:Binding Sites; D001707:Biopsy, Needle; D001943:Breast Neoplasms / Q000378:metabolism*; D002466:Cell Nucleolus / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004311:Double-Blind Method; D004967:Estrogens / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D011374:Progesterone / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*
D004967:Estrogens; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D011374:Progesterone