Optimal culture conditions for in vitro antigen-induced proliferation of rat lymph node cells.
Popliteal and inguinal lymph node cells (LNC) from rats immunized with ovalbumin (OA) in Freund's adjuvant, were cultured with OA for 4 days in a variety of serum-supplemented media. Incorporation of [3H]thymidine into [3H]DNA was assessed as an index of antigen-induced lymphoproliferation. Optimal conditions for proliferation were found to comprise culture in flat-bottomed vessels, in the presence of RPMI 1640 medium adjusted to rat osmolality (310 mosmoles/kg) containing 5% v/v fresh syngeneic rat serum plus 4 X 10(-4) M 2-mercaptoethanol.
D000818:Animals; D000941:Antigens; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005260:Female; D008198:Lymph Nodes / Q000166:cytology*; D008213:Lymphocyte Activation; D008623:Mercaptoethanol / Q000494:pharmacology; D010047:Ovalbumin / Q000276:immunology; D051381:Rats; D013601:T-Lymphocytes
D000941:Antigens; D008623:Mercaptoethanol; D010047:Ovalbumin; D004247:DNA