Development of peroxisomes in amphibians. II. Cytochemical and biochemical studies on the liver, kidney, and pancreas.
The ontogeny of catalase-containing organelles was studied by cytochemical and biochemical methods in the liver, kidney, and pancreas during the development of Rana catesbeiana. The biochemical differentiation of peroxisome in the liver and kidney was compared to that of Xenopus laevis. Catalase activity was localized after incubation in DAB medium and studied biochemically by a spectrophotometric method. In Rana Catesbeiana the number of catalase-positive organelles per cell section is low in all three organs during premetamorphosis; their number increases substantially in the liver and kidney of froglets, while it remains almost stable in the pancreas. No further increase is observed in the adult. Biochemically, the liver, kidney, and pancreas of tadpoles exhibit, respectively, 12,22 and 63% of the catalase activity found in the adult tissues. After metamorphosis an important increase of catalase activity is particularly noted in liver and kidney, the activity being, respectively, 43 and 77% of that of adult bullfrogs. On the other hand, no change in catalase activity in the liver and kidney is noted during the entire development of Xenopus laevis. The present study illustrates the very different developmental pattern of catalase activity observed during the development of two anuran amphibians. The different development pattern of the same enzyme within the small intestine, liver, kidney, and pancreas in Rana catesbeiana is also stressed.
D000375:Aging; D000818:Animals; D002374:Catalase / Q000378:metabolism; D002454:Cell Differentiation; D007668:Kidney / Q000254:growth & development* / Q000648:ultrastructure; D008099:Liver / Q000254:growth & development* / Q000648:ultrastructure; D008675:Metamorphosis, Biological; D008830:Microbodies / Q000502:physiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009928:Organ Specificity; D009940:Organoids / Q000502:physiology*; D010179:Pancreas / Q000254:growth & development* / Q000648:ultrastructure; D011892:Rana catesbeiana; D013045:Species Specificity; D014981:Xenopus
D002374:Catalase