Prevention of tolerance induction by simultaneous administration of anti-Lyb3 antiserum with tolerogen.
Lyb3 serves as a triggering receptor on a mature subset of B lymphocytes. To further investigate the nature of the activating signal received by this receptor, we examined its involvement in hapten-specific B cell tolerance induced in vivo with isologous IgG. We obtained the following results: injection of anti-Lyb3 serum simultaneously with low doses of tolerogen prevented tolerance induction in B cells responsive to TD and TI type-1 antigens but failed to influence B cells responding to a TI type-2 antigen. Furthermore, administration of anti-Lyb3 serum did not change the tolerogenic signal to an immunogenic one, allowing B cell maturation into plaque-forming cells. However, it did make the B cell responsive to a subsequent challenge with antigen. This effect is transient, and dependent on the dose of tolerogen. These results are discussed in terms of our knowledge of the mechanism of B cell tolerance.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D006241:Haptens / Q000276:immunology; D006433:Hemocyanins / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology*; D007108:Immune Tolerance; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013997:Time Factors
D000954:Antigens, Surface; D006241:Haptens; D007106:Immune Sera; D006433:Hemocyanins