Ontogeny and distribution of cells in B lineage in the American leopard frog, Rana pipiens.
Two-color immunofluorescence techniques were used in order to trace the development and distribution of cells expressing immunoglobulin in Rana pipiens. Evidence is provided which suggests that (i) embryo-larval urogenital tissues are sites of generation of cells in B lineage, (ii) during ontogeny, there is a sequential expression of immunoglobulin isotypes on B cell surfaces, (iii) larvae are able to produce the full range of immunoglobulin clases found in adults, and (iv) at least two subpopulations of lymphocytes exist in Rana pipiens, sIg+ and sIg-; thymocytes and presumably peripheral T cells lack conventional surface immunoglobulin. Some ontogenetic and phylogenetic implications are discussed.
D000818:Animals; D000921:Antibody-Producing Cells / Q000166:cytology / Q000276:immunology / Q000502:physiology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation; D005260:Female; D007074:Immunoglobulin G / Q000502:physiology; D007075:Immunoglobulin M / Q000502:physiology; D007668:Kidney / Q000166:cytology / Q000276:immunology / Q000502:physiology; D007814:Larva / Q000166:cytology / Q000276:immunology / Q000502:physiology; D008099:Liver / Q000166:cytology / Q000276:immunology / Q000502:physiology; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology / Q000502:physiology; D008297:Male; D010950:Plasma Cells / Q000166:cytology / Q000276:immunology / Q000502:physiology; D011894:Rana pipiens / Q000196:embryology / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000502:physiology
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell