Comparative effects of 60Co gamma-rays and neon and helium ions on cycle duration and division probability of EMT 6 cells. A time-lapse cinematography study.
Exponentially growing cultures of EMT 6 cells were irradiated in vitro with neon ions, helium ions or 60Co gamma-rays. Time-lapse cinematography allowed the determination, for individual cells, of cycle duration, success of the mitotic division and the age of the cell at the moment of irradiation. Irradiation induced a significant mitotic delay increasing proportionally with the delivered dose. Using mitotic delay as an endpoint, the r.b.e. for neon ions with respect to 60Co gamma-rays was 3.3 +/- 0.2 while for helium ions it was 1.2 +/- 0.1. Mitotic delay was greatest in those cells that had progressed furthest in their cycle at the time of irradiation. No significant mitotic delay was observed in the post-irradiation generation. Division probability was significantly reduced by irradiation both in the irradiated and in the post-irradiated generation. The reduction in division probability obtained with 3 Gy of neon ions was similar to that obtained after irradiation with 6 Gy of helium ions or 60Co gamma-rays.
D002453:Cell Cycle / Q000528:radiation effects*; D002455:Cell Division / Q000528:radiation effects*; D002460:Cell Line; D003037:Cobalt Radioisotopes; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006371:Helium; D007477:Ions; D009040:Motion Pictures; D009356:Neon; D013997:Time Factors
D003037:Cobalt Radioisotopes; D007477:Ions; D006371:Helium; D009356:Neon