Abnormal expression of chromosomal rabbit beta-globin gene in Saccharomyces cerevisiae.
A 5.1 kilobase-pair segment of rabbit chromosomal beta-globin DNA was joined to pJDB219, a plasmid consisting of pMB9, the 2-mu yeast plasmid and the yeast leu-2 gene. Saccharomyces cerevisiae transformed with the globin DNA-containing hybrid produced beta-globin-specific RNA. As compared to mature beta-globin mRNA, these transcripts lacked 20-40 nucleotides from the 5' end, contained all of the small intron and extended to about the middle of the large intron. Thus, no splicing of the primary beta-globin transcript could be detected in the yeast cells.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D005796:Genes; D005914:Globins / Q000235:genetics*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009694:Nucleic Acid Precursors / Q000378:metabolism; D011061:Poly A / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D004274:DNA, Recombinant; D009694:Nucleic Acid Precursors; D012333:RNA, Messenger; D011061:Poly A; D005914:Globins