Enumeration of alloreactive helper T lymphocytes which cooperate with cytolytic T lymphocytes.
The number of alloreactive helper T lymphocytes (HTL) which cooperate with cytolytic T lymphocytes have been quantified in a sensitive limit dilution system. Approximately 1 in 330 splenic T lymphocytes developed helper activity in response to alloantigen of a single haplotype. Most HTL appeared to cooperate nonspecifically with the cytolytic cells. A significant number of alloreactive HTL precursors recognized H-2 K or D alloantigen, but roughly 6-fold more HTL precursors responded to H-2 I, and roughly 20-fold more responded to non-H-2, presumably Mls, alloantigen. These results support the hypothesis that alloantigen encoded by the H-2 I and non-H-2 Mls regions preferentially stimulate helper T lymphocytes, while alloantigens encoded by the H-2 K and D regions preferentially stimulate cytolytic T lymphocytes.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D003165:Complement System Proteins; D003602:Cytotoxicity, Immunologic; D005260:Female; D006238:Haploidy; D007376:Interleukin-2 / Q000494:pharmacology; D007519:Isoantigens; D007958:Leukocyte Count; D008211:Lymphocyte Cooperation; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes
D000961:Antilymphocyte Serum; D007376:Interleukin-2; D007519:Isoantigens; D003165:Complement System Proteins