Thymoma production of T cell growth factor (Interleukin 2).
Phorbol-12-myristate-13-acetate stimulates a subline of mouse EL-4 thymoma cells to produce, in vitro, in very high titer, T cell growth factor (Interleukin 2, IL 2). The EL-4-derived IL 2 has the same m.w. (30,000) and isoelectric point heterogeneity (pI 3.8-4.4) as the IL 2 produced by Con A-stimulated spleen cells. In addition, the thymoma-derived IL 2 exhibits the same spectrum of biologic activities as has been reported for spleen cell-derived IL 2.
D000818:Animals; D002850:Chromatography, Gel; D003208:Concanavalin A / Q000494:pharmacology; D006133:Growth Substances / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis*; D008222:Lymphokines / Q000096:biosynthesis*; D051379:Mice; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013945:Thymoma / Q000378:metabolism*; D013953:Thymus Neoplasms / Q000378:metabolism*
D006133:Growth Substances; D007376:Interleukin-2; D008222:Lymphokines; D003208:Concanavalin A; D013755:Tetradecanoylphorbol Acetate