Composition of cardiolipin molecular species in Escherichia coli.
The composition of the molecular species of acidic phospholipids in Escherichia coli B during the late exponential growth phase at 37 degrees C was determined. Two phosphatidyl groups of cardiolipin, the 3-(3-sn-phosphatidyl) and 1-(3-sn-phosphatidyl) moieties of cardiolipin, were isolated by limited hydrolysis with phospholipase C. No significant difference in the composition of the molecular species was found between the 3-(3-sn-phosphatidyl) and 1-(3-sn-phosphatidyl) moieties. On the other hand, the composition of the molecular species of phosphatidylglycerol was different from that of cardiolipin. Phosphatidylglycerol contained more of the 1-palmitoyl 2-cis-9,10-methylenehexadecanoyl and 1-palmitoyl 2-cis-11,12-methyleneoctadecanoyl species than did cardiolipin. The difference in the composition of the molecular species between cardiolipin and phosphatidylglycerol may depend on the difference in the turnover rates of both phospholipids.
D002308:Cardiolipins / Q000032:analysis* / Q000378:metabolism; D004926:Escherichia coli / Q000032:analysis* / Q000378:metabolism; D007700:Kinetics; D010715:Phosphatidylglycerols / Q000032:analysis* / Q000378:metabolism; D010738:Type C Phospholipases
D002308:Cardiolipins; D010715:Phosphatidylglycerols; D010738:Type C Phospholipases