Cell-cell interaction in human granulopoiesis: role of T lymphocytes.
T lymphocytes have been implicated in the pathogenesis of granulocytopenias. We studied the effects of unstimulated and pokeweed mitogen (PWM) activated intact (Ti) and partially purified T cell subpopulations (T gamma and Tnon gamma, i.e., T mu plus T0) on in vitro granulocyte-macrophage colony formation (CFUGM) by autologous normal human bone marrow (BM). Coculture of BM and Ti, T gamma, or Tnon gamma caused only a slight decrease in the numbers of colonies and clusters; however, when cultured with a mixture of T gamma and Tnon gamma, the inhibition was significant. In contrast, activation of T cells or T cell subsets with PWM resulted in a marked decrease in colony formation. These results demonstrate that: 1) PWM-activated T cells or its subpopulations will inhibit autologous BM colony formation in vitro. The suppression seen with admixes of unstimulated T gamma and Tnon gamma is presumptive evidence that prior cell-cell interaction(s) may be required for the generation of inhibitory cells in this system; and 2) this model may represent an in vitro counterpart of immune-mediated cytopenias in man.
D000818:Animals; D001854:Bone Marrow Cells; D003114:Colony-Forming Units Assay; D004306:Dose-Response Relationship, Immunologic; D006098:Granulocytes / Q000166:cytology*; D006410:Hematopoiesis; D006801:Humans; D007141:Immunoglobulin Fc Fragments; D008211:Lymphocyte Cooperation; D009000:Monocytes / Q000166:cytology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D012397:Rosette Formation; D012756:Sheep; D013601:T-Lymphocytes / Q000166:cytology*
D007141:Immunoglobulin Fc Fragments; D011043:Pokeweed Mitogens