Hybridoma produces protective antibodies directed against the sporozoite stage of malaria parasite.
Hybrid cells secreting antibodies against sporozoites of Plasmodium berghei were obtained by fusion of plasmacytoma cells with immune murine spleen cells. The monoclonal antibodies bound to a protein with an apparent molecular weight of 44,000 (Pb44), which envelopes the surface membrane of sporozoites. Incubation of sporozoites in vitro with antibodies to Pb44 abolished their infectivity.
D000818:Animals; D000906:Antibodies; D000954:Antigens, Surface; D002999:Clone Cells / Q000276:immunology; D006822:Hybrid Cells / Q000276:immunology; D008288:Malaria / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D008970:Molecular Weight; D009194:Myeloma Proteins / Q000276:immunology; D009374:Neoplasms, Experimental / Q000276:immunology; D010954:Plasmacytoma / Q000276:immunology; D010962:Plasmodium berghei / Q000276:immunology*; D013154:Spleen / Q000276:immunology
D000906:Antibodies; D000954:Antigens, Surface; D008565:Membrane Proteins; D009194:Myeloma Proteins