[Decreased sensitivity of frog bladder cells to the antidiuretic hormone in the presence of D20].
Antidiuretic hormone (ADH) does not enhance the bladder wall permeability for the water, when 50% of the water is substituted for D2O in Ringer's solution. ADH reaction is partially reduced in the inhibition of phosphodiesterase activity by theophylline. In D2O presence cAMP-theophylline combination stimulates the water penetration, growing in proportion to an increase in Ringer's solution osmolarity under polyethylene glycol 400 action. It is suggested that D2O affects ADH-membrane receptor interaction or intensifies cAMP destruction, but the effectiveness of the subsequent intracellular reactions, stipulating augmentation of osmotic permeability, persists.
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D003903:Deuterium / Q000378:metabolism*; D066298:In Vitro Techniques; D009995:Osmosis / Q000187:drug effects; D011896:Rana temporaria; D013997:Time Factors; D001743:Urinary Bladder / Q000187:drug effects*; D014667:Vasopressins / Q000494:pharmacology*
D014667:Vasopressins; D003903:Deuterium
Umen'shenie chuvstvitel'nosti kletok mochevogo puzyria liagushki k antidiureticheskomu gormonu v prisutstvii D20.