The effect of transfer factor on spontaneous shedding of sheep red blood cell binding receptors of T lymphocytes in vitro.
Many effects of transfer factor can be used for testing of its activity in vitro. Its effect on rosette formation has been utilized in two methods: the enhancement of rosetting of trypsin-treated T lymphocytes and the increase of 'active' rosettes depressed under some immunopathological conditions. 'Active' rosetting lymphocytes of healthy blood donors if kept at 37 degrees C for 4 hr shed partly their sheep red blood cell-binding receptors into the culture medium supplemented with 25% fetal calf serum. The adding of the negative skin test-converting fraction of dialysable leucocyte extracts inhibits the decrease of the number of rosettes. Possible explanations for the observed phenomenon are: transfer factor increases the rate of receptor synthesis, it causes uncovering or redistribution of the receptors, or it stabilizes otherwise shed membrane structures.
D000818:Animals; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis / Q000378:metabolism*; D012397:Rosette Formation; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014165:Transfer Factor / Q000494:pharmacology*; D014357:Trypsin / Q000494:pharmacology
D011948:Receptors, Antigen, T-Cell; D014165:Transfer Factor; D014357:Trypsin