Investigation of insulin sensitivity in early diabetes. I. Procedure for the determination of insulin responsiveness in vivo.
Twenty-three normal weight subjects without any heredity of diabetes were characterized by a 2-hour glucose infusion test. All persons showed a normal carbohydrate tolerance and normal biphasic insulin secretion pattern. For the determination of insulin sensitivity a 1-hour priming dose--constant infusion technique was used. Two 30-minute-periods of insulin infusion (8 and 16 mU/kg, primed by a start injection of 1 and 2 mU/kg, respectively) provoked a decrease of plasma glucose and FFA concentrations by 35 +/- 12.5% and 55 +/- 30.2%, respectively. Values lower than 22.5% (glycemia) or 25% (FFA) indicate a diminished insulin responsiveness. The metabolic clearance rate of insulin did not change at several concentrations of IRI. Thus, the proposed procedure is suitable to study the insulin sensitivity in vivo.
D001786:Blood Glucose / Q000032:analysis*; D001835:Body Weight; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005951:Glucose Tolerance Test; D006801:Humans; D007328:Insulin / Q000097:blood*; D011236:Prediabetic State / Q000097:blood*; D012016:Reference Values
D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007328:Insulin