Nonspecific immunosuppressive factors in malignant ascites: further characterization and possible relationship to erythrocyte receptors of human peripheral T cells.
Nonspecific suppressor factor has been purified from malignant human ascites through partial delipidation, ammonium sulfate precipitation, ACA-22 gel filtration followed by goat anti-human IgG immunoadsorbent chromatography. Molecular weight estimated by SDS-PAGE under mild reducing conditions is 50,000 and 25,000 under vigorous reducing conditions. It inhibits PHA, Con A, and PWM responses of peripheral lymphocytes by 50% at microgram concentrations per milliliter in vitro and inhibits plaque-forming cell response to SRBC at 100 microgram/mouse in vivo. It also inhibits 2-way mixed lymphocyte reactions and natural killer cell activities. It inhibits SRBC rosette formation of peripheral T cells and appears to be an erythrocyte receptor of peripheral T cells.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D001798:Blood Proteins / Q000276:immunology; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology*; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D006041:Goats; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G / Q000276:immunology; D007164:Immunosorbents; D007166:Immunosuppressive Agents / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D050356:Lipid Metabolism; D011948:Receptors, Antigen, T-Cell; D013601:T-Lymphocytes / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D001798:Blood Proteins; D007106:Immune Sera; D007074:Immunoglobulin G; D007164:Immunosorbents; D007166:Immunosuppressive Agents; D011948:Receptors, Antigen, T-Cell