Ability of NZW but not NZB antigen-presenting cells to support T cell proliferative response to DNA methylated albumin.
The genetic contribution of the parental NZB and NZW mouse to the antigen-induced, macrophage-dependent, lymph node T cell proliferative response to d-DNA-mBSA was studied in F1 hybrid NZB/NZW (B/W) mice. Mice were immunized subcutaneously in the tail with denatured DNA-methylated BSA. Primed T cells from these mice were obtained after passage of immune periaortic and inguinal lymph node cells through nylon wool. The primed T cells were exposed in vitro to d-DNA-mBSA presented on spleen cells from BALB/c, DBA/2, NZB, NZW, or B/W mice. Immune B/W T cells gave a substantial proliferative response only in the presence of antigen-presenting cells from B/W or NZW mice. These results suggest that the response of B/W mice to DNA occurs primarily through the NZW H-2 haplotype of their own antigen-presenting cells.
D000818:Animals; D000941:Antigens / Q000276:immunology*; D002417:Cattle; D002455:Cell Division; D002478:Cells, Cultured; D004247:DNA / Q000276:immunology*; D004136:Dinitrobenzenes / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D006433:Hemocyanins / Q000276:immunology; D008297:Male; D008745:Methylation; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB / Q000235:genetics*; D010047:Ovalbumin / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology*
D000941:Antigens; D004136:Dinitrobenzenes; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D004247:DNA; D006433:Hemocyanins