Isolation of a subset of thymocytes inducible for terminal transferase biosynthesis.
A new technique using direct binding of nucleated hematopoietic cells to PNA-coated rabbit red blood cell monolayers was used to separate PNA + and PNA - cells from murine thymus. The rigorously purified PNA - thymocyte population was found to lack TdT and to be low in TL. Incubation of the negative fraction with TP 5 resulted in the synthesis of TdT in a large number of cells in the fraction, and the appearance of TL on the surface of about 20% of the cells. Isolation of this inducible population has led us to propose a new class of cells in intrathymic T cell development.
D000373:Agglutinins; D000818:Animals; D000951:Antigens, Neoplasm; D010367:Arachis; D002469:Cell Separation; D004253:DNA Nucleotidylexotransferase / Q000096:biosynthesis*; D004254:DNA Nucleotidyltransferases / Q000096:biosynthesis*; D037102:Lectins / Q000494:pharmacology; D051379:Mice; D009842:Oligopeptides / Q000494:pharmacology; D037121:Plant Lectins; D011817:Rabbits; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D000373:Agglutinins; D000951:Antigens, Neoplasm; D037102:Lectins; D009842:Oligopeptides; D037121:Plant Lectins; D004254:DNA Nucleotidyltransferases; D004253:DNA Nucleotidylexotransferase