Primary in vitro mouse B-cell response induced by self-hormone-coupled self-albumin.
[3H'-thymidine incorporation above control levels was observed when normal mouse spleen cells were cultured with pituitary hormone-coupled albumins. Most striking was the observation of proliferation with self-hormone-coupled self-albumin, for instance vasopressin-coupled mouse serum albumin. The proliferating cells were not sensitive to anti-Thy 1 antiserum plus complement and were present in nude mouse spleens. Proliferation was accompanied with the appearance of antibody-forming cells against an irrelevant antigen (sheep red blood cells). These results strongly suggest polyclonal induction of B-cell proliferation/differentiation by self-hormone-coupled self-albumin.
D000418:Albumins / Q000494:pharmacology*; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology; D001323:Autoantibodies / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010909:Pituitary Hormones, Posterior / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology; D014668:Vasotocin / Q000494:pharmacology
D000418:Albumins; D001323:Autoantibodies; D010909:Pituitary Hormones, Posterior; D001127:Arginine Vasopressin; D014668:Vasotocin