Syngeneic sensitization of mouse lymphocytes on monolayers of thyroid epithelial cells. II. T and B cell involvement in primary responses.
Using a previously developed in vitro model of sensitization of lymphoid cells against monolayers of syngeneic thyroid epithelial cells, the T or B nature of the population involved in the primary response has been investigated. For this purpose normal good responder CBA strain mice were used. T cell participation was clearly demonstrated in two ways: first, by the loss of primary sensitization after T cell depletion accomplished using several different techniques (including in vivo and in vitro anti-Thy-1.2 treatment, hydrocortisone and cyclophosphamide injections) and second, by the high response (measured by tritiated thymidine uptake) of T cell-enriched suspensions obtained either by nylon wool filtration or Ig-anti-Ig columns.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D003520:Cyclophosphamide / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D006854:Hydrocortisone / Q000494:pharmacology; D007114:Immunization; D008214:Lymphocytes / Q000145:classification / Q000276:immunology*; D008221:Lymphoid Tissue / Q000166:cytology; D051379:Mice; D008808:Mice, Inbred CBA; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology; D013961:Thyroid Gland / Q000276:immunology*
D000961:Antilymphocyte Serum; D003520:Cyclophosphamide; D006854:Hydrocortisone