title: Cryoprotection of human bone marrow committed stem cells (CFU-c) by dextran, glycerol and dimethyl sulfoxide.
abstract: Dextran, glycerol and dimethyl sulfoxide (DMSO), alone or in combination, were used for cryoprotection of human bone marrow cells. The viability of cryopreserved cells was assessed by culture of myelopoiesis-committed stem cells (CFU-c) in vitro. A significantly better protection against freezing injury was obtained by 9% dextran in combination with 3 or 5% DMSO, and also with 5 or 10% DMSO alone, than with either 15% glycerol or 9% dextran with 1% DMSO.
mesh_terms: D000818:Animals; D001141:Aromatase / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004967:Estrogens / Q000096:biosynthesis*; D006801:Humans; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D009338:Necturus maculosus; D013254:Steroid 17-alpha-Hydroxylase / Q000378:metabolism; D013329:Structure-Activity Relationship; D013737:Testis / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D004967:Estrogens; D003577:Cytochrome P-450 Enzyme System; D001141:Aromatase; D013254:Steroid 17-alpha-Hydroxylase