Identification of two granulocyte/macrophage colony-stimulating factors from porcine leukocyte cultures.
Granulocyte/macrophage colony-stimulating factors (CSF) were found in large-scale serum-free cultures of porcine blood leukocytes. The factors were concentrated from the conditioned culture medium of Concanavalin A-stimulated leukocytes using ammonium sulfate salting-out fractionation. The protein precipitate at 45-90% salt saturation contained two CSF types upon gel filtration on Sephadex G-100: One type was found to be active on mouse, but not on human bone marrow stem cells and had an apparent molecular mass of 85 000-100 000 Da; the other of about 40 000-45 000 Da stimulated both mouse and human bone marrow stem cells.
D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002850:Chromatography, Gel; D003115:Colony-Stimulating Factors / Q000302:isolation & purification* / Q000494:pharmacology; D006098:Granulocytes / Q000166:cytology; D007962:Leukocytes / Q000032:analysis*; D008264:Macrophages / Q000166:cytology; D013552:Swine
D003115:Colony-Stimulating Factors