Antibody determination by ELISA in rats with retinal S antigen-induced uveoretinitis.
Enzyme-linked immunosorbent assay was applied to the determination of the serum IgG antibody contents in rats immunized with the organ-specific autoantigen (S antigen) of the retina. Optimal conditions (i.e. S antigen concentration, serum and conjugate dilutions, enzymatic reaction time) were determined. The assay required only a single serum dilution and was well reproducible. It was very sensitive, allowing the detection of low antibody contents in animals immunized with 1 microgram of S antigen. The time course of the antibody response and its variations according to the dose and the species of the immunizing S antigen were considered.
D000818:Animals; D000941:Antigens / Q000276:immunology*; D019393:Arrestin; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D012173:Retinitis / Q000276:immunology*; D014605:Uveitis / Q000276:immunology*
D000941:Antigens; D019393:Arrestin; D007074:Immunoglobulin G