A simple reliable procedure for obtaining metaphases from human leukemic bone-marrow aspirates suitable for Giemsa banding.
Short incubation of heparinized human leukemic bone-marrow cells in phosphate buffered saline containing colcemid and overnight chilling of fixed cells yields metaphases with elongated and well-spread chromosomes. This technique enables us to do trypsin-Giemsa banding of chromosomes obtained from leukemic marrow cells otherwise difficult to band.
D001399:Azure Stains; D001853:Bone Marrow / Q000473:pathology*; D002871:Chromosome Banding; D003584:Cytological Techniques; D003703:Demecolcine / Q000494:pharmacology; D006801:Humans; D007621:Karyotyping; D007938:Leukemia / Q000473:pathology*; D008677:Metaphase; D008940:Mitotic Index
D001399:Azure Stains; D003703:Demecolcine