Mitogenic factor in human prostate extracts.
Extracts of human benign prostatic hyperplasia, well-differentiated prostatic adenocarcinoma, and normal post-pubertal prostate stimulate 3H-thymidine incorporation by resting phase cultures of fetal rat osteoblasts and fibroblasts. The stimulation is concentration dependent and reaches a maximum at twenty-four hours of incubation. Prostatic extracts are also mitogenic in cell cultures of newborn human foreskin fibroblasts and the human cell lines, BUD-8 and DoT. The growth-stimulating factor is both heat and trypsin sensitive indicating that the factor is either a protein or contains a protein moiety. The growth-stimulating activity is not related to prostatic polyamine concentration. Experiments also show the activity is not due to human prostatic acid phosphatase. A prostatic growth factor may explain the growth of fibrous nodules in benign prostatic hyperplasia and the osteoblastic response of bone to prostatic cancer.
D000135:Acid Phosphatase / Q000494:pharmacology; D000230:Adenocarcinoma / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006965:Hyperplasia / Q000378:metabolism; D007376:Interleukin-2 / Q000032:analysis*; D008222:Lymphokines / Q000032:analysis*; D008297:Male; D011467:Prostate / Q000032:analysis* / Q000378:metabolism / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism; D051381:Rats; D013997:Time Factors
D007376:Interleukin-2; D008222:Lymphokines; D000135:Acid Phosphatase