High-pressure liquid chromatographic determination of ibuprofen in plasma.
A high-pressure liquid chromatographic method is presented for the determination of ibuprofen in human plasma. Ibuprofen is extracted from plasma acidified with 1.0 M phosphoric acid using hexane containing p-phenylphenol as an internal standard. A reversed-phase octadecylsilane column was used with a liquid phase of 65% methanol and 35% 0.10 M acetate buffer (pH 5.0). A spectrofluorometric detector with an excitation wavelength of 253 nm and a band pass filter (230--420 nm) provided a detectable peak for 1 microgram of ibuprofen/ml of plasma. The effect of the pH and molarity of the mobile phase on the capacity factor was studied.
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007052:Ibuprofen / Q000097:blood*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007052:Ibuprofen