Positive and negative selection of cells by hapten-modified antibodies.
A method for simultaneous positive and negative selection of cells identified by an antibody is described. It is an adaption of the panning technique using anti-benzene arsonate (Ars)-coated Petri dishes to select the Ars-antibody-coated cells. The adherent cell population is recovered in a suitable state by hapten elution with Ars-azo-tyrosine.
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods*; D006041:Goats; D006241:Haptens / Q000276:immunology; D008214:Lymphocytes / Q000145:classification / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology; D010132:p-Azobenzenearsonate / Q000276:immunology
D000906:Antibodies; D006241:Haptens; D010132:p-Azobenzenearsonate