Temporal relationship between primary injection and cyclophosphamide administration necessary for the inhibition of the priming for a secondary response.
Using the mouse PFC anti-SRBC response, the temporal relationship between primary antigen dose and cyclophosphamide (CP) administration necessary for the inhibition of priming for the secondary IgG response was studied. The administration of CP 1 h after the primary or secondary antigen injection inhibits the responses in both cases. The administration of CP 1 h after the primary antigen injection does not inhibit priming for the secondary response; this priming is inhibited if CP is administered at 12 h or more, and the maximal degree of inhibition is induced when CP is administered 7 or 9 days after the primary antigen injection. Therefore, the proliferation stage of mature B cells to IgM secretion is not necessary for this priming. It is suggested that the switch from IgM to IgG takes place in a proliferative stage of B-cell precursors in presence of the antigen.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D001402:B-Lymphocytes / Q000276:immunology; D003520:Cyclophosphamide / Q000494:pharmacology*; D004912:Erythrocytes / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D051379:Mice; D012756:Sheep; D013997:Time Factors
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D003520:Cyclophosphamide