An automatic optical method for detection of rat lymphocyte subpopulations.
A flow cytophotometry method has been evaluated for the enumeration of rat lymphocyte subpopulations in lymph and lymph nodes. The cells were prepared by incubating them with complement and anti-Ig or anti-T antiserum, followed by trypan blue staining. Cytophotometry was used for automatic registration of dead and viable cells. Parallel viability counts were done in a microscope. In addition the cytotoxic activity of the anti-Ig antiserum was compared with its surface binding capacity in a direct immunofluorescence assay. Correlation was found between the automatic and microscopical registrations.
D000818:Animals; D002470:Cell Survival; D003602:Cytotoxicity, Immunologic; D005260:Female; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000494:pharmacology; D007958:Leukocyte Count; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000145:classification*; D008297:Male; D008643:Mesentery; D010783:Photometry / Q000379:methods; D011817:Rabbits; D051381:Rats; D011947:Receptors, Antigen, B-Cell; D013601:T-Lymphocytes / Q000276:immunology; D013897:Thoracic Duct / Q000166:cytology
D007106:Immune Sera; D011947:Receptors, Antigen, B-Cell