Cell-mediated cytotoxicity against HLA-D-region products expressed in monocytes and B lymphocytes. II. HLA-A- or B-antigen compatibility is not required for HLA-D-region directed cytotoxicity.
When effector cells were produced by sensitization in vitro with HLA-A- and B-compatible but HLA-D/DR-incompatible stimulator cells, cytotoxicity apparently was directed against a product of genes closely associated with HLA-D. For effective killing to occur with effector cells against certain minor histocompatibility antigens it is necessary that the effector cells and the target cells be matched for HLA-A or B. We have, therefore, investigated whether sharing of HLA-A or B antigens is needed to obtain efficient lysis with effector cells primed against HLA-D. Good killing was observed with effector cells against HLA-D/DRw3 and against HLA-D/DRw4 whether or not the HLA-A or B antigens were matched. The results indicated that compatibility at HLA-A or B is not required for cell-mediated cytotoxicity against a product of the HLA-D region. The HLA-D region appears to code for products that behave as strong histocompatibility antigens that can by themselves cause cell-mediated killing.
D001402:B-Lymphocytes / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006680:HLA Antigens / Q000235:genetics* / Q000276:immunology; D006648:Histocompatibility; D006801:Humans; D009000:Monocytes / Q000276:immunology*; D010641:Phenotype; D014176:Protein Biosynthesis
D006680:HLA Antigens