Production of monoclonal antibodies against MM antigen: the serologic identification of MM antigen with Ly-6.2 alloantigen.
Monoclonal antibodies against MM46, an ascitic mouse mammary tumor of C3H/H2, were produced by fusing mouse myeloma cell line NS-1 with spleen cells from a (BALB/c X C3H/HeN)F1 mice hyperimmunized with MM46, an MM antigen-positive tumor. Eight antibodies showed cytotoxicity against MM46, but not against MM48, an MM antigen-negative ascitic mammary tumor, and one hybridoma produced an agglutinating antibody. One of the cytotoxic monoclonal antibodies, 3-3-C, was selected, and the strain distribution and the tissue distribution of MM antigen were studied. The results demonstrated that MM antigen had a strain distribution identical to Ly-6.2 antigen, and a similar tissue distribution. Therefore, the characterization of MM antigen and Ly-6.2 antigen was investigated. Ly-6.2 antibody was shown to be cytotoxic for MM46, but not for MM48, in accordance with 3-3-C. Genetic segregation analysis of MM and Ly-6.2 antigens in 33 backcross mice demonstrated complete concordance between these two antigens. In addition, MM antigen phenotype of an Ly-6.2 congenic strain, C3H.B6-Ly-6b, was studied, and it was found to be positive in contrast to C3H/HeN. Furthermore, cross-absorption studies revealed that both MM46 cells and C3H.B6-Ly-6b lymph node cells could absorb cytotoxic activities of 3-3-C and monoclonal anti-Ly-6.2 antibody. The results so far obtained suggested strongly that these two loci controlling expression of MM and Ly-6.2 antigens were identical or very closely linked.
D000042:Absorption; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000912:Antibodies, Neoplasm / Q000276:immunology; D000950:Antigens, Ly / Q000235:genetics / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D003433:Crosses, Genetic; D007519:Isoantigens / Q000235:genetics / Q000276:immunology*; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000276:immunology; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008819:Mice, Nude; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000950:Antigens, Ly; D000951:Antigens, Neoplasm; D007519:Isoantigens