Detergent extraction of membrane proteins related to the action of antidiuretic hormone.
Antidiuretic hormone (ADH) induces, in the apical plasma membrane of target cells, the insertion of intramembranous particle aggregates that probably contain water channels. A mild attack of this membrane by a polyoxyethylene nonylphenyl detergent, which reversibly depressed ADH-induced water permeability, has been found to modify aggregate structure while extracting additional proteins. This simple procedure could be a valuable approach to the problem of aggregate isolation and characterization.
D000818:Animals; D007700:Kinetics; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011092:Polyethylene Glycols; D011893:Rana esculenta; D013501:Surface-Active Agents / Q000494:pharmacology*; D001743:Urinary Bladder / Q000378:metabolism*; D014667:Vasopressins / Q000494:pharmacology*
D008565:Membrane Proteins; D013501:Surface-Active Agents; D014667:Vasopressins; D011092:Polyethylene Glycols