Features of T cells causing H-2-restricted lethal graft-vs.-host disease across minor histocompatibility barriers.
Evidence is presented that T cells that produce lethal graft-vs.-host disease (GVHD) to minor histocompatibility antigens (minor HA) comprise discrete subgroups of H-2K- and H-2D-restricted T cells; double negative selection of T cells in irradiated H-2 recombinant mice was used to separate these two subgroups. No evidence could be found that I-restricted T cells contributed to GVHD, either as effector cells or helper cells. The (unprimed) precursor cells for GVHD expressed the Thy-1+, Lyt-1+/-2, Ia- phenotype. Studies in which H-2-semiallogeneic bone marrow chimeras were used as hosts for negative selection suggested that presentation of minor HA to T cells during the induction phase is controlled by marrow-derived cells; indirect evidence was obtained that these latter cells can "process" minor HA presented on H-2 different cells and thereby render the antigens immunogenic. Studies in which minor HA-different, H-2-compatible chimeras were re-irradiated and then injected with donor-vs.-host T cells suggested that the effector phase of lethal GVHD involves contact of antigen on non-marrow-derived cells.
D000818:Animals; D000950:Antigens, Ly; D002469:Cell Separation; D003602:Cytotoxicity, Immunologic; D006087:Graft vs Host Reaction; D006183:H-2 Antigens / Q000145:classification / Q000235:genetics / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009026:Mortality; D011828:Radiation Chimera; D013601:T-Lymphocytes / Q000276:immunology*
D000950:Antigens, Ly; D006183:H-2 Antigens; D006649:Histocompatibility Antigens