Purification of plasma vitamin D metabolites for radioimmunoassay.
The ability of four solvent systems to extract tritiated 25-hydroxy vitamin D3, 24,25-dihydroxy vitamin D3, 25,26-dihydroxy vitamin D3 and 1 alpha, 25-dihydroxy vitamin D3 from plasma was compared. Diethyl ether gave the highest yield of metabolites and lowest yield of "lipid" materials, the dihydroxylated metabolites were more readily extracted than 25-hydroxy vitamin D3. Following extraction with ether the vitamin D metabolites could be separated, without prior purification, on a 6.2 mm X 250 mm Zorbax-Sil high pressure liquid chromatography (HPLC) column, a simplification of previous methods. Plasma 1 alpha, 25-dihydroxy vitamin D levels measured after purification by this method were not significantly different from those obtained by an established, more complex method.
D015650:24,25-Dihydroxyvitamin D 3; D002112:Calcifediol; D002117:Calcitriol; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004100:Dihydroxycholecalciferols / Q000097:blood / Q000302:isolation & purification; D006801:Humans; D006887:Hydroxycholecalciferols / Q000097:blood / Q000302:isolation & purification; D011863:Radioimmunoassay / Q000379:methods; D012995:Solubility; D014807:Vitamin D / Q000097:blood*
D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols; D014807:Vitamin D; D015650:24,25-Dihydroxyvitamin D 3; D002117:Calcitriol; D002112:Calcifediol; C026032:25,26-dihydroxycholecalciferol