The induction of antichromatin antibodies with specificity for nuclear antigens of transformed human lymphocytes.
An antibody was produced in rabbits using human dehistonized chromatin from T lymphocytes in continuous cell culture (Jm) as immunogen. The antibody was characterized by a micro-complement fixation (CF) method against chromatin from Jm cells as well as other sources. The antibody reacted only with chromatin of transformed lymphocytes, obtained either in vitro from tissue culture, or in vivo specifically from chronic lymphocytic leukemia (CLL). This antibody did not react with chromatin of normal human or non-human lymphocytes, or with chromatin of two other related non-lymphoid human tumor types, namely chronic myelocytic leukemia (CML) and HeLa cells in continuous culture. The data suggest that there are common antigens specific to the nucleus of transformed human lymphocytes which may be related to the maintenance and/or development of this particular state of differentiation.
D000974:Antibodies, Antinuclear / Q000096:biosynthesis*; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000032:analysis*; D002843:Chromatin / Q000276:immunology*; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology; D004247:DNA / Q000276:immunology; D006801:Humans; D007945:Leukemia, Lymphoid / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*
D000974:Antibodies, Antinuclear; D000951:Antigens, Neoplasm; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004247:DNA