Cell-cell interactions in in vitro erythropoiesis.
Cell-cell interactions between T and null lymphocytes induce erythropoietin-stimulated differentiation of peripheral blood-derived erythroid progenitors. Monocytes do not inhibit the engagement of this differentiation. By the use of murine hybridoma antibodies, which are uniquely reactive with mature T lymphocytes, the dependence of immature peripheral blood BFU-E differentiation on mature T lymphocytes, or a T cell-conditioned medium is confirmed. Studies with the same antibody show that the more mature and numerous BFU-E found in marrow have no requirement for mature T cells or T cell-conditioned medium. These findings do not preclude the presence in bone marrow of immature T or even non-T cells which may serve as inducers of erythropoietin-dependent erythroid differentiation of mature marrow BFU-E.
D002450:Cell Communication; D004900:Erythroblasts / Q000166:cytology*; D004912:Erythrocytes / Q000166:cytology*; D004920:Erythropoiesis; D004921:Erythropoietin / Q000494:pharmacology; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D008214:Lymphocytes / Q000502:physiology*; D008954:Models, Biological; D009000:Monocytes / Q000502:physiology; D013601:T-Lymphocytes / Q000502:physiology*
D006454:Hemoglobins; D004921:Erythropoietin