Concanavalin A-induced alteration of surface marker expression on murine T cells.
The activation of suppressor T cells by Concanavalin A (Con A) was characterized by changes in expression of T cell surface markers. Treatment of normal mouse spleen cells with monoclonal antibody to Ly-1 prior to activation with Con A consistently reduced the resulting suppressor cell activity as assayed by mixed culture with primed spleen cells responding to sheep erythrocytes. Addition of indomethacin to anti-Ly-1 depleted spleen cells together with Con A had no effect on the resulting suppressor cell activity, whereas its addition to normal spleen cells together with Con A significantly abrogated suppressor T cell induction. In contrast, treatment of normal spleen cells with anti-Ly-1 following 24h culture with Con A had no effect on resulting suppressor cell activity. Treatment of spleen cells either prior to or following culture with Con A with monoclonal antibody to Ly-2 completely abrogated suppressor T cell activity. Suppressor T cells activated by Con A were depleted significantly by fractionation on columns containing insolubilized histamine. In contrast, fractionation of normal spleen cells on histamine columns followed by culture with Con A had no effect on subsequent suppressor T cell induction. Furthermore, direct addition of histamine (5 x 10(-4) M - 5 x 10(-8) M) to Con A preculture had no effect on resultant suppressor T cell generation, suggesting that possible immunodulatory actions of histamine may be expressed at the effector rather than during induction stages of Con A-induced suppressor T cell activation.
D000818:Animals; D000937:Antigen-Antibody Reactions; D003208:Concanavalin A / Q000494:pharmacology*; D005260:Female; D006632:Histamine / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D051379:Mice; D008808:Mice, Inbred CBA; D010641:Phenotype; D011968:Receptors, Histamine / Q000187:drug effects* / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology* / Q000648:ultrastructure
D007166:Immunosuppressive Agents; D011968:Receptors, Histamine; D003208:Concanavalin A; D006632:Histamine; D007213:Indomethacin