Surface alterations of mammalian cells upon ionizing radiation as detected by a lectin-binding technique. I. Binding of concanavalin A by blood cells of X-irradiated mice.
X-irradiation in the dose range 0.25--5 Gy induces very early dose-dependent and temporary functional alterations in the plasma membranes of murine blood cells detected by a lectin (concanavalin A)-binding technique. The irradiated erythrocytes bind 3H-concanavalin A during the first 3 hours after 0.45--4.5 Gy X-irradiation to a greater extent than the controls. The lymphocytes also react with an increased binding between 0.45 and 0.9 Gy, but a remarkable decrease is observed with 2.26 and 4.5 Gy. The platelets proved to be the most sensitive. The levels of bound radioactivity for the total platelet population and for the separated subpopulation of 'young' cells were respectively increased by a factor of four and more, after 1 Gy irradiation. The functional stimulation of plasma membranes of 'young' platelets occurred below 2 Gy, while the amount of lectin bound to 'aged' cells only increased at doses above 2 Gy.
D000818:Animals; D001665:Binding Sites; D001773:Blood Cells / Q000528:radiation effects*; D001792:Blood Platelets / Q000528:radiation effects; D002462:Cell Membrane / Q000528:radiation effects; D002470:Cell Survival; D003208:Concanavalin A / Q000378:metabolism*; D004307:Dose-Response Relationship, Radiation; D004912:Erythrocytes / Q000528:radiation effects; D008214:Lymphocytes / Q000528:radiation effects; D008297:Male; D051379:Mice; D011833:Radiation Injuries, Experimental / Q000097:blood*; D013997:Time Factors; D014316:Tritium; D014965:X-Rays
D014316:Tritium; D003208:Concanavalin A