Regulatory role of humoral factors released by macrophages in rat lymphocyte reactivity in vitro. II. Influence of humoral factors released by normal peritoneal macrophages on spontaneous lymphocyte stimulation.
Supernatants harvested from peritoneal macrophage cultures, derived from normal rats, exhibit in vitro two antagonistic activities: stimulating and suppressing spontaneous stimulation of syngeneic lymphocytes. Stimulation of DNA synthesis by macrophage supernatants occurs in lymph node lymphocytes, which show low values of spontaneous stimulation. Suppression of DNA synthesis by macrophage supernatants is revealed in thymocytes and cortisone-resistant thymocytes, which demonstrate high values of spontaneous stimulation.
D000276:Adjuvants, Immunologic; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008222:Lymphokines / Q000276:immunology*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051381:Rats; D013950:Thymus Gland / Q000166:cytology
D000276:Adjuvants, Immunologic; D007376:Interleukin-2; D008222:Lymphokines; D004247:DNA