Latex bead rosetting method for cell surface antigens.
Polystyrene latex beads coupled with anti-Ig can be used to detect the binding of specific antibodies to target cells. The optimal conditions for coupling anti-Ig to beads are described. This method is as sensitive as red cell rosetting and has the advantages that coupling is simple and the coated beads stable.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000954:Antigens, Surface / Q000032:analysis*; D005260:Female; D007840:Latex; D008297:Male; D008722:Methods; D051379:Mice; D008808:Mice, Inbred CBA; D012397:Rosette Formation
D000888:Antibodies, Anti-Idiotypic; D000954:Antigens, Surface; D007840:Latex