The haemolytic plaque assay in carp (Cyprinus carpio).
A haemolytic plaque assay for the enumeration of antibody forming cells in the carp (Cyprinus carpio) is described. Serum of bream (Abramis brama) turned out to be a more reliable complement source than allogeneic carp serum. The addition of 3--5% bream serum to a mixture of immune lymphoid carp cells and xenogeneic erythrocytes gave optimal results. Higher amounts of bream complement inhibited plaque formation. Plaque formation was also suppressed when inactive or heat-inactivated bream or carp serum was added to a mixture containing normal bream complement.
D000818:Animals; D002347:Carps / Q000276:immunology*; D002417:Cattle; D002645:Chickens; D003165:Complement System Proteins; D003530:Cyprinidae / Q000276:immunology*; D004285:Dogs; D005260:Female; D006041:Goats; D006462:Hemolytic Plaque Technique; D006736:Horses; D051379:Mice; D011817:Rabbits; D012756:Sheep; D013552:Swine; D013696:Temperature; D013997:Time Factors
D003165:Complement System Proteins