Isolation of a high molecular mass granulocyte colony-stimulating factor from bovine lung conditioned medium.
A high molecular mass type of granulocyte colony-stimulating factor (CSF-F) from bovine lung tissue produced by fibroblasts was isolated and partially purified by a four-step procedure. Following ammonium sulfate fractionation, hydrophobic interaction, ion-exchange and affinity chromatography, CSF-F was more than 200-fold enriched. CSF-F was found to be a glycoprotein of approximately 68 kDa with an isoelectric point of 4.0-5.2. It did not lose its biological activity after neuraminidase treatment. Gel-filtration behavior of desialo-CSF-F was not changed.
D000818:Animals; D001681:Biological Assay; D001853:Bone Marrow / Q000502:physiology; D002417:Cattle; D002449:Cell Aggregation / Q000187:drug effects; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D003470:Culture Media; D005347:Fibroblasts / Q000378:metabolism; D008168:Lung / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D009439:Neuraminidase
D003115:Colony-Stimulating Factors; D003470:Culture Media; D009439:Neuraminidase