Different types of endotoxin-induced release of colony stimulating factors by adherent leucocytes in the presence of fresh and heat-inactivated autologous serum.
Human adherent leucocytes stimulate in vitro granulopoiesis by releasing colony stimulating factors (CSF), which promote the growth of myeloid committed stem cells (CFUC). The effect of lipopolysaccharide (LPS) on CSF generation by adherent leucocytes was studied with fresh and heat-inactivated autologous serum. Adherent leucocyte conditioned media were fractionated on Sephadex G-75. LPS, in the presence of fresh serum, caused a significant increase of CSF release by adherent leucocytes within 1 h. Heat inactivation of autologous serum abolished this effect. Adherent leucocyte CSF had 3 activity peaks at greater than 75 000, 23 000 and less than 4 000 daltons. LPS-fresh serum initiated CSF, harvested after culture periods of 1 and 24 h, disclosed identical elution profiles.
D002448:Cell Adhesion; D002850:Chromatography, Gel; D003115:Colony-Stimulating Factors / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*
D003115:Colony-Stimulating Factors; D008070:Lipopolysaccharides