A novel pre-T cell line derived from acute lymphoblastic leukemia.
A cultured cell line, P30/Ohkubo with pre-T cell phenotype, was established from an 11-year-old girl with acute lymphoblastic leukemia (ALL). According to the morphological, cytochemical and immunological analyses, P30/Ohkubo was found to have common-ALL antigen, Ia-like antigen, pan-T cell antigen, and L2 morphology with localized acid phosphatase staining, but not rosette-forming capacity with sheep erythrocytes, B1 antigen or surface immunoglobulins. Chromosome analysis revealed that P30/Ohkubo had two constant marker chromosomes [11q +, del (9)], but lacked 14q +. These findings indicate that P30/Ohkubo is a novel cultured cell line of pre-T cell phenotype. These characteristics have remained unchanged for more than 20 months since establishment of the cell line.
D000954:Antigens, Surface / Q000032:analysis*; D002460:Cell Line; D002648:Child; D005260:Female; D005819:Genetic Markers; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D007621:Karyotyping; D007945:Leukemia, Lymphoid / Q000276:immunology* / Q000648:ultrastructure; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D005819:Genetic Markers; D000949:Histocompatibility Antigens Class II