Biochemical characterization of genetically variant aromatic amine N-acetyltransferases in A/J and C57BL/6J mice.
Biochemical genetic studies to determine the molecular basis for the differences in N-acetylation between the A/J and C57BL/6J mouse strains were carried out. Purification of liver N-acetyltransferase from both strains showed that aminofluorene and p-aminobenzoic acid activities are not separable by protein purification techniques which exploit differences in charge and size. In addition, both aminofluorene and p-aminobenzoic acid N-acetyltransferase activities from the C57BL/6J mouse liver enzymes migrated as a single symmetrical protein band after polyacrylamide disc gel electrophoresis. For aminofluorene and p-aminobenzoic acid, the two mouse strains showed apparent Km differences which suggest a difference in a structural gene product, and at least may partially account for the observed differences in N-acetylation. N-Acetyltransferase activity of selected extrahepatic tissues showed that both small intestine and kidney reflect the animal's phenotype as determined by liver or blood N-acetyltransferase. Sex differences were apparent in this activity in C57BL/6J mouse kidney, with males exhibiting approximately 2.5-fold higher activity for aminofluorene and approximately 3-fold higher activity for p-aminobenzoic acid than did females.
D010129:4-Aminobenzoic Acid; D000123:Acetyltransferases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D014644:Genetic Variation; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics; D008815:Mice, Inbred Strains / Q000235:genetics*; D013379:Substrate Specificity; D014018:Tissue Distribution
D000123:Acetyltransferases; D010129:4-Aminobenzoic Acid