Isolation of human T lymphocytes: comparison between nylon wool filtration and rosetting with neuraminidase (VCN) and 2-aminoethylisothiouronium bromide (AET)-treated sheep red blood cells (SRBC).
Isolation of human T lymphocytes by rosetting with SRBC, centrifugation on Ficoll-Hypaque gradients and lysis of SRBC, which reportedly affects the functional activities of lymphocytes, was compared to isolation of T lymphocytes by filtration through a nylon wool column. The two methods were similar in yields and purity of T lymphocytes. No selective loss of T cell subpopulations was found in T lymphocytes isolated by filtration through a nylon wool column as judged by the percentage of T gamma and T micron and the suppressor and helper activity in functional assays. Therefore, filtration through a nylon wool column is a useful method to purify T lymphocytes and has the advantage of being comparatively simple and quick to perform and of avoiding manipulations which may affect the functional activity of T lymphocytes.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D004912:Erythrocytes / Q000187:drug effects; D005374:Filtration / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008213:Lymphocyte Activation; D009439:Neuraminidase / Q000494:pharmacology*; D012397:Rosette Formation / Q000379:methods*; D012756:Sheep / Q000097:blood / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification* / Q000502:physiology; D000338:beta-Aminoethyl Isothiourea / Q000494:pharmacology*
D007074:Immunoglobulin G; D000338:beta-Aminoethyl Isothiourea; D009439:Neuraminidase