T-cell regulation of pokeweed-mitogen-induced polyclonal immunoglobulin production in mice. I. Characterization of helper T cells.
T-cell regulation of pokeweed mitogen (PWM)-induced polyclonal immunoglobulin (Ig) production was studied in mice. Under optimal stimulating conditions, about 1000 Ig-producing cells were generated per 3 x 10(5) spleen cells and macrophages were required to generate Ig-producing cells from B lymphocytes. The response of splenic B cells to PWM was partially dependent upon helper T cells, whereas the response to lymph node B cells was completely dependent upon T cells. Helper activity was attributed not only to Lyt 1+ 2- but also to Lyt 1+ 2+ T cells. Co-operation between T and B cells, in this system, was not restricted by H-2 barrier.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D007136:Immunoglobulins / Q000096:biosynthesis*; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D008211:Lymphocyte Cooperation; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D010641:Phenotype; D011043:Pokeweed Mitogens / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D007136:Immunoglobulins; D011043:Pokeweed Mitogens