During lymphatic regeneration, precursors for major histocompatibility complex-restricted cytotoxic T cells appear before alloreactive precursors.
Mice were injected with a sublethal dose of cyclophosphamide (Cy) (300 mg/kg), and the appearance of the capacity of the regenerating spleen to form cytotoxic T lymphocytes (CTL) in response against 2,4,6 trinitrophenyl-coupled syngeneic cells or against allogeneic cells was followed. It was found that 8 da after Cy injection, the spleen contained cells that could give rise to CTL, but only 2,4,6 trinitrophenyl-specific CTL responses could be obtained at this stage. A low alloresponse was first seen 2 wk after Cy injection.
D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D003520:Cyclophosphamide / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology; D008285:Major Histocompatibility Complex; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D014301:Trinitrobenzenes; D003520:Cyclophosphamide