Induction of anti-ssDNA antibodies in normal and preautoimmune mice in vivo.
Splenic lymphoid cells of normal and preautoimmune mice escape normal regulatory mechanisms in vitro and spontaneously synthesize anti-ssDNA antibody in the absence of any exogenous antigen. In addition, both the cultured cells and their cell-free supernatants were capable of inducing specific anti-ssDNA responses in vivo upon their injection into syngeneic recipients. The response was host-derived, required the participation of B and T cells, and was not due to polyclonal activation. The immunogen responsible for this response appeared to be membrane-associated, was found predominantly on cultured T cells, and was sensitive to DNase and proteolysis.
D000818:Animals; D000906:Antibodies; D000917:Antibody Formation; D001323:Autoantibodies; D001402:B-Lymphocytes / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D002474:Cell-Free System; D004277:DNA, Single-Stranded / Q000276:immunology*; D005260:Female; D006462:Hemolytic Plaque Technique; D007114:Immunization; D007700:Kinetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology
D000906:Antibodies; D001323:Autoantibodies; D004277:DNA, Single-Stranded