Transformation of human leukocytes by cocultivation with an adult T cell leukemia virus producer cell line.
The transmission of adult T cell leukemia virus, a human retrovirus, into fresh leukocytes from normal humans was examined. One of three virus-carrying cell lines, tested after being subjected to lethal x-irradiation, consistently transformed leukocytes from adult peripheral blood and umbilical cord blood. All the transformed cell lines expressed adult T cell leukemia virus-associated antigen, but transformed lines originating from adult and umbilical cord blood exhibited T cell and non-T, non-B cell surface natures, respectively. Efforts to transform human leukocytes with cell-free virus were unsuccessful.
D000954:Antigens, Surface / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D005312:Fetal Blood; D005814:Genes, Viral; D006801:Humans; D007621:Karyotyping; D007962:Leukocytes / Q000502:physiology*; D012190:Retroviridae / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology; D014170:Transformation, Genetic
D000954:Antigens, Surface; D000956:Antigens, Viral