[A simple method for detection of functional and genetics alpha-1-antitrypsin deficiencies of human serum (author's transl)].
Insoluble elastin immobilised in Agar plates was used as substrate to quantitate the elastase inhibitory capacity of human sera. This technique was found valuable to evaluate the genetic deficiency of alpha-1-antitrypsin (Z phenotypes) and also the functional loss of the elastase inhibitory capacity in smokers. A significant decrease of elastase inhibitory capacity was demonstrated in the sera of smokers between 40 and 80 years of age, as compared to non smokers of the same age. This method may be useful for the evaluation of patients suffering from elastic tissue diseases (emphysema, arteriosclerosis).
D004549:Elastin; D004800:Enzymes, Immobilized / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D010196:Pancreatic Elastase / Q000378:metabolism; D010641:Phenotype; D012907:Smoking; D000515:alpha 1-Antitrypsin / Q000235:genetics; D019896:alpha 1-Antitrypsin Deficiency
D004800:Enzymes, Immobilized; D000515:alpha 1-Antitrypsin; D004549:Elastin; D010196:Pancreatic Elastase
Méthode simple de détection des déficiences fonctionnelles et génétiques en alpha-1-antitrypsine du sérum humain.