title: Human primary lymphocyte colony formation in agar culture: polyclonal origin and significance.
abstract: Primary human lymphocyte colonies, which grow on the surface of agar culture under PHA stimulation, arise from more than one progenitor cell: they contain both type A and B of G6PD when the donor is heterozygous for the isoenzymes. The distribution pattern of electrophoretic bands indicates that, on the average, two T lymphocyte clones proliferate per colony. Colony formation in the present circumstances appears as an in vitro system more suitable for the study of cellular cooperation between a limited number of cells than for the characterization of immature lymphocyte precursors in man.
mesh_terms: D000362:Agar; D002455:Cell Division; D002478:Cells, Cultured; D002999:Clone Cells / Q000166:cytology / Q000201:enzymology; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology* / Q000201:enzymology
chemical_list: D007376:Interleukin-2; D000362:Agar; D005954:Glucosephosphate Dehydrogenase