Purification and characterization of a monoclonal T-cell suppressor factor specific for poly(LGlu60LAla30LTyr10).
A monoclonal T-cell-derived suppressor factor specific for the terpolymer poly(LGlu60LAla30LTyr10) produced by the T-cell hybridoma 258 C4.4, was purified to homogeneity. This was accomplished by fractionation of the culture medium by using a combination of affinity chromatography and reverse-phase and ion-exchange high-performance liquid chromatography. The purified factor is composed of a single Mr 24,000 polypeptide chain, and the homogeneous protein maintains the ability to suppress antibody and T-cell proliferative responses to poly(LGlu60LAla30LTyr10) specifically. The specific activity of pure suppressor factor is calculated to be 8 X 10(7) units/micrograms.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D006825:Hybridomas; D008222:Lymphokines / Q000302:isolation & purification* / Q000502:physiology; D051379:Mice; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D010455:Peptides / Q000276:immunology*; D011108:Polymers; D013491:Suppressor Factors, Immunologic; D013601:T-Lymphocytes
D000596:Amino Acids; D008222:Lymphokines; D010455:Peptides; D011108:Polymers; D013491:Suppressor Factors, Immunologic; C020749:GAT