Lymphotoxin and chemotactic factor produced in vitro by human lymphocytes during their proliferative response in the presence of phytohaemagglutinin and Nocardia water-soluble mitogen.
The present investigation was initiated for the purpose of elucidating which cell type, T and/or B lymphocytes, were responsible for human lymphotoxin (LT) and human chemotactic factor (CF) production in vitro. T- and B-enriched subpopulations were tested for proliferative responses and lymphokine release in the presence of a human T mitogen, phytohaemagglutinin (PHA), and a human B-mitogen, Nocardia water-soluble mitogen (NWSM). We found that PHA-stimulated T cells produced LT and CF whereas NWSM-stimulated B-enriched subpopulation released LT and CF.
D001402:B-Lymphocytes / Q000276:immunology; D002469:Cell Separation; D002630:Chemotactic Factors / Q000096:biosynthesis*; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000145:classification*; D008233:Lymphotoxin-alpha / Q000096:biosynthesis*; D008934:Mitogens / Q000494:pharmacology; D009615:Nocardia / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology
D002630:Chemotactic Factors; D008233:Lymphotoxin-alpha; D008934:Mitogens; D010835:Phytohemagglutinins