The influence of autologous erythrocytes on active T-cells rosetting with sheep red blood cells and on DNA synthesis.
The addition of autologous erythrocytes to a mixture of human peripheral blood lymphocytes and sheep red blood cells (SRBC) alters the spontaneous rosetting reaction in the test detecting the active fraction of T lymphocytes (ARFC). Increase in rosetting is more often observed in mixtures containing smaller human red blood cell: lymphocyte ratios, while inhibition occurred in those containing a higher ratio. Autologous erythrocytes also affected DNA synthesis when added to three-day lymphocyte cultures, unstimulated or stimulated with BCG. These effects were more strongly pronounced when phagocytic cells were removed from lymphocytes tested.
D000818:Animals; D001500:BCG Vaccine / Q000276:immunology; D004247:DNA / Q000096:biosynthesis*; D004306:Dose-Response Relationship, Immunologic; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D009000:Monocytes / Q000276:immunology; D012397:Rosette Formation; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology*
D001500:BCG Vaccine; D004247:DNA