Monoclonal antibodies to hemagglutinin and to H-2 inhibit the cross-reactive cytotoxic T cell populations induced by influenza.
The target antigens recognized by the cross-reactive population of cytotoxic T cells on A/USSR/90/77 (H1N1) influenza virus-infected cells was studied with monoclonal antibodies to the H-2k antigen and viral hemagglutinin. The cytotoxic killing of virus-infected cells was differentially inhibited by three monoclonal antibodies to H-2k. Synergistic inhibition of cytotoxic activity was obtained with monoclonal antibodies to H-2 and to some antigenic determinants on the hemagglutinin melecule of A/USSR but not with others. Since target cells infected with other subtypes of influenza A viruses were not inhibited by any of the monoclonal antibodies to A/USSR hemagglutinin, it is suggested that the sites recognized by the cross-reactive cytotoxic T cells were sterically inhibited by the anti-A/USSR monoclonal antibodies. The results suggest that at least one of the target antigens recognized by the cross-reactive population of cytotoxic T cells is located on the hemagglutinin molecule. The present observations are in agreement with cytotoxic T cell recognition of closely situated viral and H-2 determinants.
D000373:Agglutinins; D000818:Animals; D000906:Antibodies; D002999:Clone Cells / Q000276:immunology; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens; D006385:Hemagglutination Inhibition Tests; D006388:Hemagglutinins; D009980:Influenza A virus / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D009976:Orthomyxoviridae Infections / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology
D000373:Agglutinins; D000906:Antibodies; D006183:H-2 Antigens; D006388:Hemagglutinins; D014764:Viral Proteins