A monoclonal antibody specific for immature human hemopoietic cells and T lineage cells.
An anti-human monoclonal antibody (RFB-1) has been produced that reacts with a group of hemopoietic precursor cells in human bone marrow. These include terminal deoxynucleotidyl transferase-positive (TdT+) cells and myeloid colony-forming unit cells, functionally identifiable progenitor cells of the granulocytic-monocytic series. The expression of RFB-1 antigen on myeloid cells decreases as the cells become more mature; myeloblasts are weakly RFB-1+ but most promyelocytes are RFB-1-. RFB-1 also reacts with TdT+ thymic blast cells and cortical thymocytes but is unreactive with TdT- medullary thymocytes, although the majority of peripheral T cells are weakly RFB-1+. RFB-1 is the first anti-precursor cell reagent that labels human TdT+ cells in both the thymus and bone marrow as well as hemopoietic precursor cells but is unreactive with pre-B blasts and B lymphocytes.
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001773:Blood Cells / Q000166:cytology; D001853:Bone Marrow / Q000378:metabolism; D002469:Cell Separation; D002645:Chickens; D003114:Colony-Forming Units Assay; D006041:Goats; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D008214:Lymphocytes / Q000145:classification; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D014066:Palatine Tonsil / Q000166:cytology; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology
D000911:Antibodies, Monoclonal