Quantitative estimate of unlabelled cordycepin in acid-soluble pool isolated from rat brain tissue after intraperitoneal injection of the inhibitor.
A technique for the quantitative estimation of intraperitoneally injected unlabelled cordycepin in an acid-soluble pool (ASP) isolated from rat brain tissue is suggested. It consists in consecutive chromatography of ASP on Dowex 1 X 8, Dihydroxyboryl = SP500 and Sephasorb-HP. The fraction containing 2'-deoxyriboadenosine and 3'-deoxyriboadenosine (cordycepin) has been isolated from brain tissue ASP of experimental animals after a cordycepin injection. 2'-Deoxyriboadenosine fraction has been isolated from tissue ASP of the control animals not subjected to an inhibitor injection. Brain tissue antibiotic content has been estimated by the difference in nucleoside quantity values (microM) in these two fractions (control/experiment).
D000818:Animals; D001923:Brain Chemistry; D002845:Chromatography; D003839:Deoxyadenosines / Q000032:analysis* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D051381:Rats; D012995:Solubility; D013053:Spectrophotometry
D003839:Deoxyadenosines