Features of cells controlling H-2-restricted presentation of antigen to T helper cells in vivo.
Information was sought on the nature of cells presenting antigen (sheep red blood cells) in an H-2-restricted fashion to T helper cells in vivo. The approach used was to examine the capacity of F1 hybrid T cells to collaborate with parental strain B cells after prior positive selection of the T cells to antigen in irradiated parental strain mice in the presence of various sources of Thy 1-negative cells taken from F1 or parental strain mice. The results showed that H-2-restricted presentation of antigen to T cells is controlled by radioresistant, nylon-wool adherent. Thy 1-negative cells, which are enriched in spleen and peritoneal exudates, rare in lymph node cell suspensions, and undetectable in thoracic duct lymph; pretreatment in vitro with anti-Ia reagents directed either to I-A or I-E subregion determinants abolished antigen-presenting activity. These findings correlate closely with the known characteristics of cells that present antigen in vitro. Studies in which re-irradiated parent leads to F1 chimeras were used as hosts for positive selection suggested that nonhematopoietic cells do not play a discernible role in antigen presentation.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002469:Cell Separation; D003433:Crosses, Genetic; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; D007116:Immunization, Passive; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011828:Radiation Chimera; D013601:T-Lymphocytes / Q000276:immunology*
D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II