Simultaneous determination of p-aminobenzoic acid, acetyl-p-aminobenzoic acid and p-aminohippuric acid in serum and urine by capillary gas chromatography with use of a nitrogen-phosphorus detector.
In various studies during recent years, the use of p-aminobenzoic acid has been described in screening tests for exocrine pancreatic function. A synthetic three-unit compound N-benzoyl-L-tyrosyl-p-aminobenzoic acid has been administered orally and hydrolysed in the small intestine in the presence of chymotrypsin to N-benzoyl-L-tyrosine and p-aminobenzoic acid. This study describes a convenient procedure in which, after a selective extraction and derivatization with diazomethane, capillary gas chromatography is used combined with nitrogen-sensitive detection. With the proposed procedure, p-aminobenzoic acid and its major metabolites, acetyl-p-aminobenzoic acid and p-aminohippuric acid, can be monitored in serum and in urine samples.
D010129:4-Aminobenzoic Acid / Q000032:analysis* / Q000097:blood / Q000652:urine; D062365:Aminobenzoates / Q000032:analysis*; D000618:Aminohippuric Acids / Q000032:analysis*; D002849:Chromatography, Gas; D006801:Humans; D009584:Nitrogen; D010758:Phosphorus; D010130:p-Aminohippuric Acid / Q000032:analysis* / Q000097:blood / Q000652:urine; D062366:para-Aminobenzoates
D062365:Aminobenzoates; D000618:Aminohippuric Acids; D062366:para-Aminobenzoates; C023730:Acedoben; D010758:Phosphorus; D009584:Nitrogen; D010129:4-Aminobenzoic Acid; D010130:p-Aminohippuric Acid