Induction of O-deethylase activity as an index to exposure to coal-derived products and trace environmental pollutants.
The metabolism of the synthetic substrate 7-ethoxyresorufin is a selective measure of the activity of cytochrome P-448 monooxygenase, the subset of cytochrome P-450-mediated enzymes preferentially induced by polycyclic aromatic hydrocarbons and related compounds. 7-Ethoxycoumarin metabolism, on the other hand, reflects total (nonselective) cytochrome P-450 monooxygenase activity. Either substrate yields a single, highly fluorescent product, amenable to direct, sensitive assay with the portable centrifugal analyzer. We used three assays with liver microsomes from C57/BL6 mice for the short-term bioassay of the dose-dependent effects of exposure to selected environmental toxins, including petroleums, polychlorinated biphenyls, and their oxidative degradation products.
D015660:7-Alkoxycoumarin O-Dealkylase; D000818:Animals; D001140:Aroclors / Q000633:toxicity; D003031:Coal; D003577:Cytochrome P-450 Enzyme System; D004785:Environmental Pollutants / Q000633:toxicity*; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D010105:Oxygenases / Q000096:biosynthesis*; D010578:Petroleum / Q000633:toxicity; D010634:Phenobarbital / Q000494:pharmacology
D001140:Aroclors; D003031:Coal; D004785:Environmental Pollutants; D010578:Petroleum; D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; D015660:7-Alkoxycoumarin O-Dealkylase; D010634:Phenobarbital