Gene transfer in intact animals.
Resistance to methotrexate was induced in bone marrow cells of mice by transformation in vitro with DNA from a drug-resistant cell line. Transformed cells were injected in vivo and haematopoietic cells expressing resistance were selected by drug treatment of recipients. Transformed cells had elevated levels of dihydrofolate reductase and demonstrated a proliferative advantage over untransformed cells, indicating successful gene transfer.
D000818:Animals; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002455:Cell Division; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004351:Drug Resistance; D005796:Genes; D005818:Genetic Engineering / Q000379:methods*; D006410:Hematopoiesis / Q000187:drug effects; D008727:Methotrexate / Q000494:pharmacology; D051379:Mice; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D014170:Transformation, Genetic
D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate