Characterization of lymphocyte subpopulations which exhibit enhanced DNA synthesis in vitro in DNFB contact sensitive guinea-pigs.
Different lymphocyte subpopulations were prepared from lymph nodes and spleen obtained from 2.4-dinitrofluorobenzene/Freund's complete adjuvant (DNFB/FCA) sensitized guinea-pigs. The capacity to react in vitro to secondary antigenic (dinitrophenyl-modified syngeneic macrophages, DNP-MO) stimulation by an increased DNA synthesis, appeared to be restricted to those T cells which lack receptors for IgG-Fc (T gamma -). We confirmed the existence in this species of an IgG-Fc receptor-positive T-cell subset, which is particularly represented in the spleen (up to 56%). These T gamma + cells apparently are not of monocyte lineage as they show strong PHA-responsiveness and a lack of non-specific esterase staining, whereas the majority of these cells form RRBC-rosettes. Neither B nor T gamma + cells appeared to act as suppressor cells in the secondary DNP-MO-driven proliferative response.
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004139:Dinitrofluorobenzene / Q000276:immunology; D005260:Female; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D008297:Male; D011961:Receptors, Fc / Q000032:analysis; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology / Q000378:metabolism
D007074:Immunoglobulin G; D011961:Receptors, Fc; D004247:DNA; D004139:Dinitrofluorobenzene