Similar kinetic characteristics of 5-hydroxytryptamine binding in blood platelets and brain membranes of rats.
Blood platelets and brain membranes of SIV and Lewis rats both exhibited two saturable binding sites for 5-hydroxytryptamine (5-HT) in the concentration range 1-100 nM. The Kd values for the high-affinity sites were 4-6 nM and for the low-affinity sites 20-40 nM in both tissues of both rat strains. Blood platelets had 100-200 times more binding sites per mg protein (Bmax) than brain membranes. Thus, 5-HT receptors of platelets may be used as models for those of cerebral 5-HT-neurons.
D000818:Animals; D001665:Binding Sites; D001792:Blood Platelets / Q000032:analysis / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D005260:Female; D007700:Kinetics; D008566:Membranes / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D011985:Receptors, Serotonin / Q000032:analysis; D012701:Serotonin / Q000378:metabolism*
D011985:Receptors, Serotonin; D012701:Serotonin