title: Measurement of canine von Willebrand factor using ristocetin and Polybrene. Diagnosis of canine von Willebrand's disease.
abstract: Dogs with VWD provide useful models for the study of the factor VIII complex. However, the study of canine VIIIR:WF has been hampered by the lack of a routine plasma assay for canine RCF, an activity that is usually used as a measure of VIIIR:WF. This study shows that canine plasma can be assayed for RCF with a macroscopic tilt-tube method using formalin-fixed human platelets, ristocetin, and extra canine albumin (5.0 mg/ml) to prevent plasma precipitation. An assay was also developed for canine plasma PBCF, an activity that is closely related to RCF. In 45 normal canine plasmas, VIII:C was not correlated with RCF, PBCF, or VIIIR:AG. However, RCF, PBCF, and VIIIR:AG were well correlated with each other. In 26 canine VWD plasmas, VIII:C was frequently normal, whereas VIIIR:AG, RCF, and PBCF were almost always deficient. The patterns of VIIIR:AG, RCF, and PBCF deficiencies in the canine VWD plasmas suggested that some canine breeds have a "classic" form of VWD whereas other breeds have "variant" forms of disease.
mesh_terms: D000818:Animals; D001777:Blood Coagulation; D001779:Blood Coagulation Factors / Q000032:analysis; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis*; D004285:Dogs; D006583:Hexadimethrine Bromide / Q000097:blood; D006801:Humans; D012310:Ristocetin / Q000097:blood; D014842:von Willebrand Diseases / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D014841:von Willebrand Factor / Q000032:analysis*
chemical_list: D001779:Blood Coagulation Factors; D014841:von Willebrand Factor; D012310:Ristocetin; D006583:Hexadimethrine Bromide