Preparative fractionation of T and B lymphocytes of the Syrian golden hamster with soybean agglutinin.
T and B lymphocytes of the Syrian golden hamster were separated from spleen cell preparations on the basis of their differential agglutinability with the lectin soybean agglutinin. Only B lymphocytes were agglutinated by this lectin, and they could be separated from the unagglutinated T lymphocytes by sedimentation through 50% heat inactivated calf serum at unit gravity. The B lymphocyte aggregates could be dissociated into single cells that were viable and functional after treatment with 0.5 M galactose. The isolated cell fractions were characterized by their blastogenic response to various T cell and B cell specific mitogens and by the presence or absence of cell surface IgG.
D000818:Animals; D001402:B-Lymphocytes / Q000032:analysis*; D002469:Cell Separation; D005591:Chemical Fractionation / Q000379:methods*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D006224:Cricetinae / Q000097:blood*; D037102:Lectins / Q000494:pharmacology*; D008647:Mesocricetus / Q000097:blood*; D037121:Plant Lectins; D011947:Receptors, Antigen, B-Cell; D013025:Glycine max; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000032:analysis*
D037102:Lectins; D037121:Plant Lectins; D011947:Receptors, Antigen, B-Cell