Cytosolic and mitochondrial phosphoenolpyruvate carboxykinase of the bullfrog, Rana catesbeiana, liver.
The mitochondrial and cytosolic phosphoenolpyruvate carboxykinase (GTP: oxaloacetate carboxy-lyase(transphosphorylating), EC 4.1.1.32) occurring in the bullfrog (Rana catesbeiana) liver were studied. The enzymes in th two intracellular compartments of both tadpole and adult frog liver were immunologically identical. Both radioactively-labelled forms of the mitochondrial and cytosolic phosphoenolpyruvate carboxykinase from bullfrog liver were imported at the same rate into intact mitochondria in vitro. The mitochondrial and cytosolic enzyme activities did not respond to the administration of glucagon, glucocorticoid, quinolinate and D-mannoheptulose which are known as enhancers of phosphoenolpyruvate carboxykinase, but were found to increase during natural metamorphosis. The former activity was markedly increased in the tadpoles treated with 3,5,3'-triiodothyronine. It was supposed that in the bullfrog liver the phosphoenolpyruvate carboxykinase localized in the mitochondria is of central importance in phosphoenolpyruvate synthesis from oxaloacetate.
D000818:Animals; D003600:Cytosol / Q000201:enzymology; D007106:Immune Sera; D007118:Immunoassay; D007700:Kinetics; D007814:Larva / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D008675:Metamorphosis, Biological; D008930:Mitochondria, Liver / Q000201:enzymology*; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism*; D011892:Rana catesbeiana; D013367:Submitochondrial Particles / Q000201:enzymology
D007106:Immune Sera; D010729:Phosphoenolpyruvate Carboxykinase (GTP)