Experimental photoallergic contact dermatitis: a mouse model.
We have induced photoallergic contact dermatitis in mice to 3,3',4',5 tetrachlorosalicylanilide (TCSA), chlorpromazine and 6-methylcoumarin. These compounds are known to produce photoallergic contact dermatitis in humans. The photoallergic contact dermatitis reaction in the mouse is immunologically specific viz. mice photosensitized to TCSA react, by photochallenge, to that compound and not to chlorpromazine, and conversely. The reaction requires UVA at both sensitization and challenge. It appears to be T-cell mediated in that it can be passively transferred to syngeneic mice by lymph node cells from actively sensitized mice, the histology of the reactions resembles that of classic allergic contact dermatitis in mice, challenge reactions are seen at 24 but not at 4 hr, and photoallergic contact dermatitis can be induced in B-cell deficient mice. The availability of a mouse model for the study of photo-ACD will facilitate the identification of pertinent control mechanisms and may aid in the management of the disease. It is likely that a bioassay for photoallergens of humans can be based on this mouse model.
D000818:Animals; D002746:Chlorpromazine / Q000009:adverse effects; D003374:Coumarins / Q000009:adverse effects; D003877:Dermatitis, Contact / Q000209:etiology / Q000276:immunology*; D004195:Disease Models, Animal; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D010787:Photosensitivity Disorders / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D012458:Salicylanilides / Q000009:adverse effects; D013601:T-Lymphocytes / Q000276:immunology; D014466:Ultraviolet Rays / Q000009:adverse effects*
D003374:Coumarins; D012458:Salicylanilides; C018096:6-methylcoumarin; C003390:3,3',4',5-tetrachlorosalicylanilide; D002746:Chlorpromazine