Synthesis in E. coli of a polypeptide with human leukocyte interferon activity.
Double-stranded cDNA prepared from the 12S fraction of poly(A) RNA from interferon (IF)-producing human leukocytes was cloned in Escherichia coli using the pBR322 vector. One of the resulting clones had a 910-base pair insert which could hybridise to IF mRNA and was responsible for the production of a polypeptide with biological IF activity. Up to 10,000 units IF activity per g of cells was obtained from some clones.
D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D007372:Interferons / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D007962:Leukocytes / Q000378:metabolism*; D010957:Plasmids; D014170:Transformation, Genetic
D004274:DNA, Recombinant; D007372:Interferons