title: HLA antigens expressed on human pluripotent hemopoietic precursors in vitro (CFUMIX).
abstract: The human major histocompatibility antigen HLA, plays an important role in transplantation. To ascertain the expression of the antigens of HLA-A and -B loci on the surface of human pluripotent hemopoietic progenitors (CFUMIX), the cytotoxic effect of specific anti-HLA sera was examined. Anti-HLA sera and complement formed fewer colonies from CFUMIX in a culture medium than the controls which were treated with autologous serum. In our experiments, the HLA antisera killed also T cells that carry the surface antigen. Then the influences of T cells on mixed colony formation were examined. No reduction of colony formation was observed from the cell suspension in which T cells were depleted by treatment of monoclonal antibody OKT3. These results indicated that CFUMIX expressed HLA antigens on their surface.
mesh_terms: D000954:Antigens, Surface / Q000276:immunology; D002454:Cell Differentiation; D003114:Colony-Forming Units Assay; D005260:Female; D006680:HLA Antigens / Q000276:immunology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007518:Isoantibodies / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000954:Antigens, Surface; D006680:HLA Antigens; D007106:Immune Sera; D007518:Isoantibodies