Differential synchronization of leukemic cells in vivo by hydroxyurea.
Flow cytometry (FCM) was used to monitor the cell cycle changes which occurred in the bone marrow cells of a patient with acute myeloblastic leukemia following pre-induction treatment with hydroxyurea. Flow cytometric and cytogenetic analysis showed two populations of cells; one hypertetraploid and presumably leukemic, and the other diploid and possibly normal. Sequential FCM monitoring of bone marrow cells after hydroxyurea demonstrated an early rise (after 24 h) in the S-phase component of diploid cells and a rise in the S-phase component of hypertetraploid cells 48-72 h later. Conventional induction therapy timed to coincide with this latter peak resulted in early remission.
D002453:Cell Cycle / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000473:pathology*; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D011123:Polyploidy
D006918:Hydroxyurea