Rabbit spleen B lymphocytes as helper cells in lymphocyte activation by concanavalin A and phytohaemagglutinin.
Using rosetting methods, we have purified rabbit B cells and studied their interactions with T cells purified by passage over an anti-immunoglobulin-coated Degalan beads column. B cells enhance the response of T cells to concanavalin A (Con A) and phytohaemagglutinin. In regulation of the response to Con A, an adherent cell is a third participating cell. B-cell preparation contain a minority of cells that can respond to T mitogens with the help of non-proliferating T cells, but the proportion of these responding cells is small, and the involvement of the T-cell impurity cannot be excluded.
D000818:Animals; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology* / Q000528:radiation effects; D002448:Cell Adhesion; D002469:Cell Separation; D003208:Concanavalin A / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D008213:Lymphocyte Activation / Q000528:radiation effects; D008211:Lymphocyte Cooperation; D010835:Phytohemagglutinins / Q000494:pharmacology; D011817:Rabbits; D013154:Spleen / Q000166:cytology*; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology / Q000528:radiation effects
D010835:Phytohemagglutinins; D003208:Concanavalin A