Anti-mite IgE antibody production in vitro by peripheral blood lymphocytes from mite-sensitive atopic patients.
Anti-mite IgE antibody production in vitro was investigated using peripheral blood lymphocytes (PBL) from mite-sensitive patients with bronchial asthma. Preculture of PBL with the mite antigen, which induced the remarkable cell proliferation, resulted in a marked decrease of the IgE antibody production directed to mite but not to unrelated antigens. Anti-mite IgE antibody formation was not inhibited by autologous or allogeneic T cells stimulated with the mite antigen. The spontaneous production of IgE antibody was observed when fractionated B cells were cultured alone or with pokeweed mitogen (PWM), but was suppressed when they were precultured with the antigen. Coculture of autologous T cells with B cells showed a considerable augmentation of anti-mite IgE antibody formation. The enhancing activity of autologous T cells was not observed when B cells were irradiated at 1,000 rad. The spontaneous IgE antibody production by B cells alone was radioresistant. Both mitotic inhibitors and 1,000 to 2,000 rad of gama-irradiation did not significantly suppress the spontaneous anti-mite IgE antibody production by B cells.
D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D003561:Cytarabine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008925:Mites / Q000276:immunology*; D008937:Mitomycins / Q000494:pharmacology; D008938:Mitosis / Q000187:drug effects; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology
D000941:Antigens; D008937:Mitomycins; D011043:Pokeweed Mitogens; D003561:Cytarabine; D003609:Dactinomycin; D007073:Immunoglobulin E; D003571:Cytochalasin B