title: Monoclonal antibody assessments of T cell interactions in erythropoietin studies.
abstract: The contemporary application of clonal assay techniques has greatly expanded our knowledge of the regulation of hematopoiesis. Our efforts have been directed toward the investigation of non-erythropoietin-mediated regulation of human erythropoiesis in the form of cell-to-cell interaction between mature T cells and erythroid progenitors. Our data indicate that three such progenitors, the early marrow erythrocyte precursor BFU-E, the more mature marrow erythrocyte precursor, CFU-E and the peripheral blood BFU-E, each exhibit totally different requirements for their colony expression in culture, with respect to the absence of erythropoietin and the presence of mature T cells or their products. The capacity of erythroid progenitors to withstand incubation in the absence of erythropoietin appears to be a characteristic of immature rather than mature erythroid progenitors. Furthermore, use of OKT3 antibody depletion techniques shows that peripheral blood-derived BFU-E appear to depend upon mature lymphocytes or T cell-conditioned medium for erythropoietin-stimulated differentiation while bone marrow BFU-E and CFU-E have no requirement for mature T cells to produce erythropoietin-dependent maturation. Our results, plus a vast array of data provided by other investigators in the field, are integrated into a proposed framework for further investigation of T cell induction of erythropoietin-dependent erythroid differentiation aimed at more specifically identifying the inducer cell subset(s) in that system.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001853:Bone Marrow / Q000187:drug effects; D002450:Cell Communication; D003470:Culture Media; D004912:Erythrocytes / Q000166:cytology; D004920:Erythropoiesis; D004921:Erythropoietin / Q000494:pharmacology*; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D003470:Culture Media; D004921:Erythropoietin