[Significance of the spontaneous rosette formation test with mouse erythrocytes in the complex quantitative analysis of human B-lymphocytes].
Some mechanisms were studied that are concerned with mouse red blood cell rosette formation by human peripheral blood lymphocytes. Lymphocytes rosetting with mouse red blood cells (EM--RFC) were identified by the test similar to the E-rosette formation technique. EM--RFC accounted for 7.7+/-0.48% in the peripheral blood of healthy donors. It has been established by stepwise selective inhibition of Fc- and C3-receptors and surface immunoglobulins that human Fc- and C3-receptors do not take part in rosetting with mouse red blood cells, whilst surface immunoglobulins, particularly IgM or near-located structures, are implicated in the reaction of lymphocytes with mouse red blood cells. Multiple analysis of lymphocyte receptors has been performed in patients with different immunologic disorders. A significant decrease in the EM--RFC level was found in patients with rheumatoid arthritis. In patients with dys-and hypoimmunoglobulinemia, the content of EM--RFC varied depending on the severity of immunity B-system injury and correlated well with the cells bearing surface immunoglobulins. The drastically increased number EM--RFC (up to 34.6--78%) was recorded in the blood of patients with chronic lympholeukemia. These findings served as basis for applying EM--RFC detection for diagnosing various forms of lympholeukemia.
D000361:Agammaglobulinemia / Q000276:immunology; D000818:Animals; D001172:Arthritis, Rheumatoid / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation / Q000379:methods; D003176:Complement C3; D004406:Dysgammaglobulinemia / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007945:Leukemia, Lymphoid / Q000276:immunology; D051379:Mice / Q000276:immunology; D011951:Receptors, Complement / Q000276:immunology; D011961:Receptors, Fc / Q000276:immunology; D012397:Rosette Formation
D003176:Complement C3; D011951:Receptors, Complement; D011961:Receptors, Fc
Znachenie testa spontannogo rozetkoobrazovaniia a éritrotsitami mysheĭ v kompleksnom kolichestvennom analize B-limfotsitov cheloveka.