[Proposal for a technic for demonstrating T lymphocytes on a frozen section. Marking by membrane fragments of sheep red blood cells].
T lymphocytes may be identified in the blood and in suspensions derived from lymphoid tissue, by their tendency to form rosettes with sheeps' red blood cells. Some authors have described a method of obtaining the E rosettes in histological sections (Whiteside and Rowlands, 1977; Brubaker and Whiteside, 1977, 1979; Sakamoto and Oda, 1979). We demonstrate that it is possible to fix fragments of the membrane of sheep's red blood cells obtained by thermal shock. This fixation may be demonstrated by fluorescent or electron microscopy. It is possible to demonstrate T lymphocytes in sections by using membrane from fragmented sheeps' red blood cells.
D000818:Animals; D004910:Erythrocyte Membrane / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D005615:Freezing; D006041:Goats; D006801:Humans; D008856:Microscopy, Fluorescence; D011817:Rabbits; D012397:Rosette Formation / Q000379:methods*; D012756:Sheep / Q000276:immunology; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology*
Proposition de technique de mise en évidence de lymphocytes T sur coupe á congélation. Marquage par des fragments de membrane de globules rouges de mouton.