title: Binding of surface IgG by immunocompetent thymus cells.
abstract: We have previously shown that myeloma-derived or serum-derived immunoglobulin could facilitate in vitro alloantigen recognition by immunocompetent thymus cells ('subset-I thymocytes'). The present report demonstrates the isolation of surface immunoglobulins from radiolabeled splenic B lymphocytes. Solubilized radiolabeled preparations of surface immunoglobulin are shown to bind in vitro to lymphoid cells, especially to immunocompetent thymus cells. Immunocompetent thymus cells (subset-I thymocytes preferentially bind more IgG than IgM. Papain digests of IgG are also bound by subset-I thymocytes; preferential binding of the Fc piece is observed. Although the binding of IgG or its pieces per se does not activate lymphoid cells in vitro a role for the binding of IgG during alloantigen recognition in vitro between immunocompetent thymus cells is demonstrated.
mesh_terms: D000818:Animals; D001666:Binding Sites, Antibody; D002417:Cattle; D002450:Cell Communication; D007074:Immunoglobulin G / Q000378:metabolism; D007457:Iodine Radioisotopes; D007519:Isoantigens; D008211:Lymphocyte Cooperation; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism*; D011961:Receptors, Fc; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
chemical_list: D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D007519:Isoantigens; D011947:Receptors, Antigen, B-Cell; D011961:Receptors, Fc