Two T lymphocyte subpopulations isolated from human peripheral blood following "in vitro" treatment with adenosine.
Incubation of human peripheral blood lymphocytes (PBL) with adenosine resulted in a decrease in the level of E-rosette forming cells (ERFC). Isolated ERFC rerosetted with sheep erythrocytes in the presence of adenosine yielded two T lymphocyte subpopulations: a major one, rosetting or "E(R)" and a minor one, non-rosetting or "E(S)" T cells. Characterization of the two isolated subpopulations revealed that both E(R) and E(S) cells were positive for human T lymphocyte antigen. However, in contrast to E(R) cells, E(S) cells had low rosetting capacity, high spontaneous thymidine incorporation and low PHA proliferative response.
D000225:Adenine / Q000494:pharmacology; D000241:Adenosine / Q000494:pharmacology*; D000328:Adult; D000818:Animals; D006801:Humans; D066298:In Vitro Techniques; D010835:Phytohemagglutinins / Q000494:pharmacology; D012397:Rosette Formation; D012756:Sheep; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D013806:Theophylline / Q000494:pharmacology
D010835:Phytohemagglutinins; D013806:Theophylline; D000225:Adenine; D000241:Adenosine