title: [A microagar culture system for clonal growth of human T-lymphocytes].
abstract: A simple and reproducible micro-agar culture system for cloning human T lymphocytes has been described. The system consists of an agar layer and a liquid phase, which enables the easy addition of test substances and a prolonged proliferation. Further advantages of this system are a low incubation volume and a better morphological, cytochemical, immunological and electron-microscopical study of colonies obtained from the liquid phase.
mesh_terms: D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000648:ultrastructure; D008854:Microscopy, Electron; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D003470:Culture Media; D037102:Lectins
vernacular_title: Ein Mikroagarkultursystem für das klonale Wachstum von menschlichen T-Lymphozyten.