Cytochemical reactions in resting and activated T-lymphocytes.
Peripheral blood T-lymphocytes from normal persons were studied before and after incubation with phytohemagglutinin (PHA) for the presence of beta-glucuronidase, alpha-naphthyl acetate esterase, alpha-naphthyl butyrate esterase, acid phosphatase, and periodic acid-Schiff (PAS)-positive material. The number of T-lymphocytes containing beta-glucuronidase, alpha-naphthyl acetate esterase,  and alpha-naphthyl butyrate esterase was reduced (P < 0.005) after incubation with PHA. No significant change in the number of T-lymphocytes positive for acid phosphatase was observed. PAS-positive material was markedly increased (P < 0.005) in activated T-lymphocytes. The data suggest that the cytochemical profile of resting T cells differs markedly from that of activated T-lymphocytes.
D000135:Acid Phosphatase / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002478:Cells, Cultured; D004950:Esterases / Q000378:metabolism*; D005966:Glucuronidase / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D008213:Lymphocyte Activation; D008247:Lysosomes / Q000201:enzymology; D009283:Naphthol AS D Esterase / Q000378:metabolism; D010503:Periodic Acid-Schiff Reaction; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000201:enzymology* / Q000473:pathology
D010835:Phytohemagglutinins; D004950:Esterases; D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases; C021855:naphthylbutyrate esterase; D000135:Acid Phosphatase; D005966:Glucuronidase