Development and improvement of a commercial uric acid enzymatic determination kit on a centrifugal fast analyzer.
The performance of a uric acid determination kit has been evaluated for five months, under routine conditions, in a General Hospital Biochemical Laboratory. An anomalous increment in fresh serum blanks was noted in the kit when first introduced. This interference, probably due to alcohol dehydrogenase contamination, was corrected by addition of 50 mmol/l (NH4)2SO4 to the reagent. Results obtained with this modified reagent correlate perfectly with those obtained with modified kits by Smith Kline Instruments (SKI), and with many other determination methods. Correlations are discussed and explanations for differences in statistical data are offered. Within run and between run precision data are presented. The kit fits perfectly with the needs of centrifugal fast analyzers and discrete micro analyzers, on account of its speed, reliability and precision.
D000429:Alcohol Oxidoreductases / Q000494:pharmacology; D000645:Ammonium Sulfate / Q000494:pharmacology; D001322:Autoanalysis / Q000379:methods; D002498:Centrifugation; D005189:False Positive Reactions; D005969:Glutamate Dehydrogenase / Q000494:pharmacology; D006801:Humans; D011933:Reagent Kits, Diagnostic; D013223:Statistics as Topic; D014503:Urate Oxidase; D014527:Uric Acid / Q000097:blood*
D011933:Reagent Kits, Diagnostic; D014527:Uric Acid; D000429:Alcohol Oxidoreductases; D005969:Glutamate Dehydrogenase; D014503:Urate Oxidase; D000645:Ammonium Sulfate