Synthesis of ribosomal protein S1 following nutritional shift-up in Escherichia coli K-12.
The synthesis rate of ribosomal protein S1 was measured in Escherichia coli K-12 during the transitional period following a nutritional shift-up from acetate minimal to glucose/amino acids/nucleosides medium. The synthesis rate of S1 increased without a lag suggesting that the S1 gene is under stringent control and located very close to its promoter. The rate of S1 synthesis slowed between 7 and 15 min, and then increased to the postshift-up steady state rate. The postshift-up steady state rate was half the initial rate obtained between 0 and 7 min. The slow down in the synthesis rate between 7 and 15 min indicates that an unknown factor(s), in addition to guanosine 5'-diphosphate, 3'-diphosphate, participates in the regulation of S1 gene expression.
D000085:Acetates / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D009705:Nucleosides / Q000378:metabolism; D014176:Protein Biosynthesis; D012269:Ribosomal Proteins / Q000096:biosynthesis*
D000085:Acetates; D000596:Amino Acids; D009705:Nucleosides; D012269:Ribosomal Proteins; D005947:Glucose