A high yield purification procedure for alpha and beta chains of HLA-DR antigens.
A procedure is described for purifying to homogeneity the alpha and beta chains of HLA-DR antigen from the homozygous human lymphoid cell line LG-2. The procedure involves a combination of lectin affinity as well as classical and high pressure liquid chromatography. From 100 g of packed cells, 8 mg (235 nmol) of alpha chain and 7 mg (205 nmol) of beta chain were isolated.
D000595:Amino Acid Sequence; D001402:B-Lymphocytes; D002460:Cell Line; D006023:Glycoproteins / Q000302:isolation & purification; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000302:isolation & purification*; D006801:Humans; D046911:Macromolecular Substances
D006023:Glycoproteins; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D046911:Macromolecular Substances