Fluorescence activated cell sorter analysis of murine B cell development.
Three subpopulations of surface immunoglobulin positive B cells were defined using monoclonal antibodies directed against IgM and IgD antigenic determinants and 2-color fluorescence activated cell sorter analysis: cells with a high density of sIgM and lacking sIgD (M++D-); cells with slightly less sIgM and dully positive for sIgD (M++D+), and cells with a low density of sIgM but brightly staining with anti-IgD (M+D+). In the spleen of two-week-old animals the M++D- cells are the more numerous. Their frequency declines during development in favor of the adult M+D+ cells. Because the densities of sIgM and sIgD on M+D+ cells are positively correlated it is hypothesized that immature M++D- cells first lose their sIgM and then reaquire sIgM together with sIgD.
D000375:Aging; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000145:classification / Q000166:cytology* / Q000276:immunology; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry; D007072:Immunoglobulin D / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal; D007072:Immunoglobulin D; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell