Identification of T lymphocytes in human mixed hemopoietic colonies.
The addition of a T-cell growth-promoting medium (PHA-TCM) to culture conditions that support growth of multi-lineage hemopoietic colonies enhances the proliferation of cells with lymphoid morphology within these colonies. These cells were identified as T lymphocytes by their ability to form rosettes with SRBC and their reaction with monoclonal antibodies (OKT3, OKT4) directed against T-cell-specific surface components. They continue to proliferate extensively under the influence of PHA-TCM after transfer of mixed colonies into liquid suspension culture. Supportive evidence for a common progenitor of myeloid and lymphoid cells within single mixed colonies is provided by Y-chromatin body analysis of E-rosette positive and negative cells in colonies grown in cocultures of male and female bone marrow cells.
D002455:Cell Division; D002478:Cells, Cultured; D004912:Erythrocytes / Q000166:cytology; D005260:Female; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells; D006801:Humans; D008297:Male; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000166:cytology; D014998:Y Chromosome