title: Lymphokine production in primary mixed lymphocyte reactions. I. Characteristics of responding and stimulating cells.
abstract: The characteristics of the responder and stimulating cells involved in migration inhibition factor (MIF) production in primary 'one-way' mouse mixed lymphocyte reactions (MLR) were analyzed by using an indirect agarose droplet assay. T-lymphocytes are mainly responsible for MIF release, as shown by pretreatment with anti-Thy 1.2 serum plus complement or purification over a nylon wool column. On the other hand, macrophages and B-lymphocytes appear to be optimal stimulating cells. T-lymphocytes as stimulating cells induce MIF release, but to a much lesser degree than macrophages and B-lymphocytes. The kinetics of MIF production in MLR is related to the kind of stimulating cells employed. Lastly, the ability to release MIF is already present in the spleen of 1- to 2-week-old mice, lasts until 20 weeks of age and declines to undetectable levels at 50 weeks of age.
mesh_terms: D000375:Aging; D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D007106:Immune Sera / Q000494:pharmacology; D007700:Kinetics; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis*; D008222:Lymphokines / Q000096:biosynthesis*; D008264:Macrophages / Q000378:metabolism; D008297:Male; D051379:Mice; D013268:Stimulation, Chemical; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D007106:Immune Sera; D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines