Monoclonal CLL B-cells may be induced to grow in an in vitro B-cell colony assay system.
A simple reproducible in vitro B-cell colony assay system was used to evaluate B-cell growth in controls and patients with chronic lymphocytic leukemia (CLL). All six CLL patients studied formed B-cell colonies. The number of colonies was significantly less in patients than controls (66 +/- 18 versus 127 +/- 8). CLL colonies were shown to be monoclonal and appeared to reflect the circulating malignant B-cell clone in our patient group, while the six controls studied formed polyclonal B-cell colonies. Wright-Giemsa staining showed typical plasma cells to have developed in the controls but not in the patients. Cells from CLL patients retained a more lymphoid appearance. It is believed that investigations with this B-cell assay will provide the means for further in vitro evaluation of malignant B-cell proliferation in other lymphoproliferative disorders.
D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002455:Cell Division; D003114:Colony-Forming Units Assay / Q000379:methods; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007945:Leukemia, Lymphoid / Q000276:immunology / Q000473:pathology*
D007136:Immunoglobulins