title: Metabolism of thyroxine in Rana catesbeiana tadpoles during metamorphic climax.
abstract: Previous studies have indicated that premetamorphic tadpoles do not convert T4 to T3 to a measurable extent (1). The present study was performed to determine whether a T4 5'-monodeiodinating system is acquired at later stages of development. [125I]T4 metabolism in vivo was determined in tadpoles at most stages of prometamorphosis and metamorphic climax and, for comparison, in premetamorphic tadpoles. The conversion of [125I]T4 to [125I]T3, as indicated by the presence of an 125I-labeled product in serum and liver preparations that cochromatographed with carrier T3, was sometimes observed in tadpoles near the end of prometamorphosis and was always evident in tadpoles that were either undergoing or had completed metamorphic climax. However, during this phase, no correlation could be drawn between the extent of T3 production and morphological development. The formation of T3 from T4 in vivo was significantly decreased in tadpoles pretreated with propylthiouracil. The T45'-monodeiodinating system could be induced in premetamorphic tadpoles by injecting them with either T4 or T3. This finding together with the observation that normal acquisition of this system occurs at the time when endogenous T4 and T3 levels are rising rapidly suggest that its development is under the control of the thyroid hormones.
mesh_terms: D000818:Animals; D007454:Iodides / Q000378:metabolism; D007457:Iodine Radioisotopes; D008099:Liver / Q000378:metabolism; D008675:Metamorphosis, Biological; D011441:Propylthiouracil / Q000494:pharmacology; D011892:Rana catesbeiana; D013974:Thyroxine / Q000378:metabolism*; D014284:Triiodothyronine / Q000378:metabolism
chemical_list: D007454:Iodides; D007457:Iodine Radioisotopes; D014284:Triiodothyronine; D011441:Propylthiouracil; D013974:Thyroxine