Cryopreserved human cultured T cells as responder cells for the quantitative measurement of interleukin-2: improvement of the assay.
Large batches of human cultured T cells (CTC) were cryopreserved for later use as responder cells in a proliferation assay for measurement of interleukin (IL)-2 activity. Cryopreservation of CTC could be carried out without considerable loss in viability and cryopreserved and fresh cells showed equally good responses to IL-2. The conditions of IL-2-dependent CTC growth were analyzed, which led to a better evaluation of test results, and had important implications for the calculation of relative IL-2 activity. The repeated use of the same batch of cryopreserved CTC reduced test variability and provided an assay system that allows reliable and reproducible measurement of human IL-2 activity.
D002478:Cells, Cultured; D003451:Cryoprotective Agents; D006801:Humans; D007376:Interleukin-2 / Q000032:analysis*; D007700:Kinetics; D011309:Preservation, Biological; D013601:T-Lymphocytes
D003451:Cryoprotective Agents; D007376:Interleukin-2