[Adenosine deaminase activity in T-enriched and T-depleted preparations of human peripheral lymphocytes].
Adenosine deaminase activity has been determined in T-enriched and T-depleted preparations of peripheral mononuclear cells from 6 normal subjects. T-enriched populations showed ADA levels lower than unseparated mononuclear cells (1.84 +/- 0.30 vs. 2.36 +/- 0.59 nmol NH3/min/10(6) cells). The highest values were found in T-depleted populations (2.46 +/- 0.39). Considering T cells percentages in our mononuclear cell preparations, T cells account for 64% of the biochemical ADA activity of peripheral blood mononuclear cells and the remaining 36% is due to non-T cells. The method for cell separation and enzymatic activity determination we used could be highly suitable for monitoring ADA levels in pathological conditions.
D000243:Adenosine Deaminase / Q000097:blood*; D000293:Adolescent; D000328:Adult; D002469:Cell Separation; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D009000:Monocytes / Q000201:enzymology*; D009700:Nucleoside Deaminases / Q000097:blood*; D013601:T-Lymphocytes / Q000201:enzymology*
D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase
Attività adenosina deaminasi in preparazioni T-arricchite e T-deplete di linfociti umani periferici.