Essential and nonessential cooperative signals in lymphocyte triggering in solid cultures.
The kinetics of lymphocyte stimulation by mitogens in solid cultures were used to study the role of cell interactions in these responses. An absolute requirement for cell interactions, possibly through soluble mediators, was found in lymphocyte responses to calcium ionophore A23187, phytohemagglutinin HA17 and tetradecanoylphorbol acetate. Accessory leukocytes were adherent to plastic surfaces and did form E rosettes. Autologous erythrocytes were also capable of providing essential cooperative signals. 2-Mercaptoethanol, in contrast, only provided nonessential cooperative signals for lymphocyte stimulation in solid cultures.
D000001:Calcimycin / Q000494:pharmacology; D002448:Cell Adhesion; D003470:Culture Media; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D007962:Leukocytes / Q000276:immunology; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008623:Mercaptoethanol / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D012397:Rosette Formation; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D003470:Culture Media; D010835:Phytohemagglutinins; D000001:Calcimycin; D008623:Mercaptoethanol; D013755:Tetradecanoylphorbol Acetate