Cytokinetic and cytogenetic effects of some agricultural chemicals on human lymphoid cells in vitro: organophosphates.
In the present study, cytotoxic, cytostatic and cytogenetic effects of a number of organophosphate pesticides on human lymphoid cells (LAZ-007) in culture have been examined. Cytotoxic effects were dose related and often led to extensive cell kill. The pronounced effects of various organophosphates on the cell-cycle traverse were shown in data based on the enumeration of M1 and M3 metaphases after incubation of cells with BrdU. In cells incubated with 20 micrograms/ml of the various chemicals, the number of M1 metaphases ranged from 6% (R-1303) to 18% (Azodrin) compared to no M1 metaphases in control cultures. The number of M3s in cultures treated with 20 micrograms/ml of the various chemicals tested varied from 0% (Phosdrin) to 7% (parathion) as compared to 17% in control cultures. 11 out of the 14 organophosphates tested, significantly increased the SCE frequency. Of the 9 chemicals tested after metabolic activation by liver microsomal S9 preparation, significant increases in SCE frequency were seen in diazinon-, dimethoate-, Dursban- and Phosdrin-treated cells.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000502:physiology*; D001711:Biotransformation; D002455:Cell Division; D002460:Cell Line; D002877:Chromosomes, Human / Q000187:drug effects; D003520:Cyclophosphamide / Q000494:pharmacology; D006801:Humans; D007306:Insecticides / Q000378:metabolism / Q000494:pharmacology*; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013329:Structure-Activity Relationship
D007306:Insecticides; D003520:Cyclophosphamide