Arylsulphatase C and sterol sulphatase activities in microsomes from human placenta.
Microsomes of human placenta were tested with respect to sulphohydrolase activity towards p-nitrophenyl sulphate, oestrone sulphate, dehydroepiandrosterone sulphate and pregnenolone sulphate. Microsomal sulphohydrolases were solubilized by treatment with 0.5% Triton X-100 at pH 9.0. Bio-gel A 1.5 m column chromatography allowed to separate the microsomal arylsulphatase into subfractions differing in molecular mass and substrate specificity. Oestrone sulphate sulphohydrolase appeared to be as specific for microsomal fraction as glucose-6-phosphatase.
D001192:Arylsulfatases / Q000378:metabolism*; D002845:Chromatography / Q000379:methods; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008861:Microsomes / Q000201:enzymology*; D017830:Octoxynol; D010920:Placenta / Q000201:enzymology* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000494:pharmacology; D011247:Pregnancy; D043266:Steryl-Sulfatase; D013429:Sulfatases / Q000378:metabolism*
D011092:Polyethylene Glycols; D017830:Octoxynol; D013429:Sulfatases; D001192:Arylsulfatases; D043266:Steryl-Sulfatase