Peanut agglutinin. V. Thymocyte subpopulations in the mouse studied with peanut agglutinin and Ly-6.2 antiserum.
Thymocyte heterogeneity has been studied by the use of two cell surface markers--the Ly-6.2 alloantigen and peanut agglutinin (PNA) receptor. The previously described distribution of Ly-6 on corticoresistant and PNA binding to corticosensitive thymocytes has been confirmed. Double immunofluorescence labeling experiments in combination with techniques of physical enrichment for "mature" thymocytes have resulted in the identification of a novel subset of T cells carrying both the Ly-6 antigen and PNA receptors. Purification by fluorescence cell sorting of corticosteroid-treated thymocytes, labeled with monoclonal anti-Ly-2.2 antiserum, for phenotypically mature Ly-1+23-lymphocytes causes a concomitant enrichment in the proportion of Ly-6-positive cells, which lack the PNA receptor. The implications of these findings for the maturational pathways within the thymus are discussed.
D000305:Adrenal Cortex Hormones / Q000494:pharmacology; D000373:Agglutinins; D000818:Animals; D010367:Arachis; D001665:Binding Sites; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000494:pharmacology*; D007519:Isoantigens / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010641:Phenotype; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification*; D013950:Thymus Gland / Q000276:immunology
D000305:Adrenal Cortex Hormones; D000373:Agglutinins; D007106:Immune Sera; D007519:Isoantigens