Cyclosporin receptor on mouse lymphocytes.
Specific binding of [3H]-cyclosporin C (3H-CS-C), an immunosuppressive oligopeptide, was characterized on different mouse lymphocytes. The binding was saturable, time-dependent and reversible; and KD of 1.5 x 10(-7) M and maximum binding capacity Bmax of 35 pmol/4 x 10(6) cells was found for thymocytes. A computerized analysis of the data confirmed a single population of high affinity binding sites. Lymphocytes of different organs differed in their Bmax: thymus greater than spleen greater than mesenteric lymph node (pure T cells) greater than mesenteric lymph node (pure B cells). Erythrocytes did not show a specific binding.
D000818:Animals; D001667:Binding, Competitive; D002478:Cells, Cultured; D005260:Female; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D011971:Receptors, Immunologic / Q000378:metabolism* / Q000494:pharmacology; D013601:T-Lymphocytes / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
D011971:Receptors, Immunologic; C028752:cyclosporin receptor; D014316:Tritium