title: Genetic analysis of a pleiotropic mutant of Klebsiella pneumoniae affected in nitrogen metabolism.
abstract: Genetic and reversion analyses of a thermosensitive pleiotropic mutant strain of Klebsiella pneumoniae with defects in nitrogen fixation and nitrogen metabolism have shown that the pleiotropie behaviour of mutant is due to a single mutation in a gene designated nim. This gene is contransducible with trp at a frequency of about 30% (using bacteriophage P1) and with cys at a frequency of about 14%. The gene order is cys, trp, nim. The defect in the nim mutant is complemented by the E. coli F' element, F'148.
mesh_terms: D002874:Chromosome Mapping; D005816:Genetic Complementation Test; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism*; D009591:Nitrogenase / Q000378:metabolism; D010641:Phenotype; D014161:Transduction, Genetic
chemical_list: D009591:Nitrogenase; D009584:Nitrogen