Lyt phenotypes of primary cytotoxic T cells generated across the A and E region of the H-2 complex.
Six different cell-mediated lympholysis (CML) combinations were established, four of which generated effector cells against the I-E, and two against the I-A molecule. The cell surface phenotype of effector cells was then determined by depletion of cytotoxic T lymphocyte (CTL) activity with antisera and rabbit complement (C) treatment. Both types of effector cells were completely eliminated by treatment with anti-Thy-1.2 antiserum plus C. Anti-Lyt-1.2 and C depleted anti-A and anti-E killer activity but did not eliminate CTL generated across a whole H-2 difference. One out of three different batches of anti-Lyt-2.2 antiserum did not deplete anti-A killer activity, while it efficiently eliminated CTL generated across the E region or whole H-2 difference. However, two batches of anti-Lyt-2.2 antiserum depleted also anti-A CTL activity. A quantitative difference between anti-A and anti-E CTL in terms of Lyt-2 expression was demonstrated by significant differences in recovery of killer activity, after treatment of these two types of CTL with a wide concentration range of the same anti-Lyt-2.2 antiserum and C. Thus it is concluded that anti-A killer cells have the cell surface phenotype of Thy-1+, Lyt-1, Lyt-2, whereas anti-E CTL are Thy-1+, Lyt-1, Lyt-2. The data are discussed in the context of a possible association of Lyt phenotypes of T cells with the type of MHC antigens they recognize.
D000818:Animals; D003602:Cytotoxicity, Immunologic; D005260:Female; D006183:H-2 Antigens / Q000235:genetics*; D007106:Immune Sera / Q000494:pharmacology; D007519:Isoantigens / Q000235:genetics*; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D051379:Mice; D010641:Phenotype; D014176:Protein Biosynthesis; D013601:T-Lymphocytes / Q000276:immunology*
D006183:H-2 Antigens; D007106:Immune Sera; D007519:Isoantigens