Identification of rat T and B lymphocytes by surface marker analysis.
The majority of thymocytes in suspension (72.1%) formed nonimmune rosettes with guineapig erythrocytes but not with erythrocytes from sheep, cats, dogs, pigs, or man. In contrast, only a minority of cells form lymph node (2.1%) or spleen (1.4%) rosetted with guineapig erythrocytes. Treatment of guineapig erythrocytes with neuraminidase of 2-S-aminoethyl-isothiouronium bromide did not enhance rosette formation. Adherence of the erythrocytes to tissue sections was achieved in the thymic cortex and T-cell-dependent areas of lymph node and spleen. Absorbed equine anti-rat antithymocyte serum bound 98% thymocytes and 70.0, 35.1 and 44.6% lymphocytes from peripheral blood, spleen and lymph nodes respectively. Surface immunoglobulin was demonstrated on 2.2% thymocytes, 35% peripheral-blood lymphocytes and 43 and 51% cells from lymph nodes and spleen respectively, Complement receptors were determined in suspension with erythrocyte-antibody-complement complexes. Rosetting was observed in 44% splenic, 29% lymph node and 3% thymocyte cellular suspension. The presence of Fc receptors for IgG was assessed by determining the pattern of binding of erythrocyte-antibody complexes to frozen tissue sections. For rats, antithymocyte serum is the method of choice for T cells, whereas SIg determination is the most reliable B-cell marker.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002415:Cats; D002417:Cattle; D002478:Cells, Cultured; D004285:Dogs; D004912:Erythrocytes / Q000276:immunology; D006168:Guinea Pigs; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D011951:Receptors, Complement / Q000276:immunology; D011961:Receptors, Fc / Q000276:immunology; D012397:Rosette Formation; D012756:Sheep; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D007074:Immunoglobulin G; D011951:Receptors, Complement; D011961:Receptors, Fc