Human B lymphocytes form rosettes after insertion of T lymphocyte membrane constituents.
Isolated plasma membranes from human thymocytes or peripheral T lymphocytes upon their reconstitution with envelope glycoproteins of Sendai virus, afforded membrane vesicles that fused efficiently with human peripheral B cells. The b cells thus obtained acquired the ability to form regular rosettes at 4 degrees C with sheep red blood cells. However, only B cells modified by fusion with thymocyte membranes formed stable rosettes at 37 degrees C, a property which distinguished thymocytes from peripheral T cells. Transfer of membrane components between human cells provides a new approach for the investigation of the sheep red blood cell receptor as well as the structure-function relationship of other lymphocyte membrane components.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002459:Cell Fusion; D002462:Cell Membrane / Q000502:physiology; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology*; D012397:Rosette Formation; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology*; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000502:physiology
D008565:Membrane Proteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins