A differentiation antigen expressed selectively by a proportion of human blood cells: detection with a monoclonal antibody.
A monoclonal antibody, FMC 8, has been prepared after immunization with a cell line of the non-T-non-B type acute lymphoblastic leukaemia (ALL). The antigen detected by this antibody consists of a single polypeptide chain with an apparent molecular weight of 24,000 d. The antigen is absent from T cells but appears after mitogen stimulation. It is found on the majority of normal circulating B cells but is absent in most chronic lymphocytic leukaemias. All cells of the non-T-non-B type express the antigen, whereas T-ALL cells generally do not. Granulocytes, monocytes and platelets react with the antibody, but erythrocytes do not. The wide but selective distribution of the antigen renders FMC 8 potentially useful, together with other monoclonal antibodies, in the delineation of differentiation pathways and in phenotypic analysis of leukaemia and lymphoma cells.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009000:Monocytes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm