The effect of mucolytic agents on the rheologic and transport properties of canine tracheal mucus.
The effect of several sulfhydryl and other agents on the rheologic and mucociliary transport properties of a model secretion, reconstituted canine tracheal mucus, was investigated. The mucus was obtained via the canine tracheal pouch. Rheologic properties were determined by mirorheometry, and the ciliary transport rate was determined using the frog palate technique. It was found that N-acetyl cysteine decreased the elastic modulus, leading to improved mucociliary transport at concentrations such that the mucin did not precipitate. S-carboxymethyl cysteine had no effect on either mucus properties or mucociliary transport rate, and its reported effectiveness in vivo must be due to some mechanism other than solubilization of mucin. Similar results were found with other blocked sulfhydryl compounds. Urea and potassium iodide to decrease mucus elasticity, but are harmful to cilia at the concentrations needed.
D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D002233:Carbocysteine / Q000494:pharmacology; D003545:Cysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D004285:Dogs; D004548:Elasticity; D005100:Expectorants / Q000494:pharmacology*; D066298:In Vitro Techniques; D009093:Mucus / Q000502:physiology*; D011193:Potassium Iodide / Q000494:pharmacology; D011892:Rana catesbeiana; D012212:Rheology; D014132:Trachea / Q000378:metabolism*; D014508:Urea / Q000494:pharmacology; D014783:Viscosity
D005100:Expectorants; D011193:Potassium Iodide; D002233:Carbocysteine; D014508:Urea; C026826:S-ethylcysteine; D003545:Cysteine; C017507:mecysteine; D004229:Dithiothreitol; D000111:Acetylcysteine