Computerised acquisition and analysis of flow cytometric data.
An interface between a commercial flow cytometer (Ortho Cytofluorograf 4800) and a Desk Top Computer (H.P. 9845S) is described. Programs for data acquisition and analysis have been developed. These include cell DNA content analysis, 2-parameter (3D) histograms and sophisticated sub-population selection. Although cell sorting is not possible using the facility, the data acquired per cell is optimised and detailed analysis can be carried out. The system represents a flexible, easily controlled and economical method for storing and analysing flow cytometric data.
D002477:Cells / Q000032:analysis / Q000166:cytology; D003201:Computers; D003584:Cytological Techniques / Q000295:instrumentation; D004247:DNA / Q000032:analysis; D012212:Rheology
D004247:DNA