title: Suppression splenic T lymphocytes in human hepatosplenic Schistosomiasis mansoni.
abstract: Splenic suppressor cell activity was evaluated in 10 patients with advanced hepatosplenic Schistosomiasis mansoni undergoing elective splenectomy. We used cell mixing experiments to assess the effect of mitomycin-C-treated spleen cells on antigen and mitogen-induced 3H-thymidine incorporation of responder cells. The suppressor to responder ratio was 1.0. Spleen cells from 7 of 10 patients caused at least 20% suppression of phytohemagglutinin-induced 3H-thymidine incorporation of one or more populations of responder cells (spleen cells, autologous and allogeneic peripheral blood mononuclear cells)Responses of peripheral blood mononuclear cells to streptokinase-streptodornase and schistosome soluble egg and worm antigen preparations also were inhibited by co-cultured spleen cells. An inverse correlation was apparent between the spleen cell response to PHA and the suppressor activity of that spleen cell population (r = -0.74, p less than 0.05). Cell purification procedures showed that the active suppressor splenic cell was non-adherent and rosetted neuraminidase-treated sheep erythrocytes. This splenic suppressor T lymphocyte may modulate splenic and peripheral blood lymphocyte responses in patients with hepatosplenic schistosomiasis.
mesh_terms: D000293:Adolescent; D000328:Adult; D002448:Cell Adhesion; D002648:Child; D005260:Female; D006801:Humans; D008109:Liver Diseases, Parasitic / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D008937:Mitomycins / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D012397:Rosette Formation; D012550:Schistosoma mansoni / Q000276:immunology; D012552:Schistosomiasis / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013158:Splenic Diseases / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013936:Thymidine / Q000378:metabolism
chemical_list: D008937:Mitomycins; D010835:Phytohemagglutinins; D013936:Thymidine