Alloreactive cloned T cell lines. III. Accessory cell requirements for the growth of cloned cytolytic T lymphocytes.
We examined the role of accessory cells that cooperated with the cloned cytolytic T cell line, L3. Accessory cells and T cell growth factors (TCGF) acted synergistically to stimulate cloned L3 CTL to grow and produce cytolytic activity. Accessory cells were non-T, radioresistant, plastic adherent M phi-like cells that interacted with cloned L3 CTL via soluble factors. Peritoneal M phi, at low density, also cooperated with L3 cells but at high density, inhibited L3 cells. Both accessory and inhibitory M phi acted upon L3 CTL without the intervention of other T lymphocytes or nonadherent cells. Other published reports indicate that M phi may promote cytolytic activity indirectly, amplifying TCGF production by helper T lymphocytes. This report indicates that M phi may also promote cytolytic activity directly, amplifying the response of CTL to TCGF.
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D002463:Cell Membrane Permeability; D002999:Clone Cells / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005260:Female; D006133:Growth Substances / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*
D006133:Growth Substances