In vitro sensitivity of CFU-L to cytosine arabinoside (Ara-C).
The in vitro sensitivity of leukemic colony-forming cells to Cytosine arabinoside (Ara-C) was assessed in acute myeloid leukemia, by means of a clonogenic assay using methyl-cellulose with phyto-hemagglutinin (PHA)-leukocyte conditioned medium. Bone marrow clonogenic cells were more sensitive to Ara-C than blood clonogenic cells. Also a continuous exposure to Ara-C induced a higher reduction of the leukemic colonies than one hour exposure. Patients were, according to the in vitro results, divided into 3 groups: eight patients had a dose-responsive curve for all the tested drug concentrations and were characterized by a marked sensitivity to the induction treatment combining Ara-C and Adriamycin. Three patients had a = 50% reduction of colonies with the 3 concentrations; two achieved complete remissions. Seven patients were in vitro insensitive to the drug; four of them had a relative or absolute resistance to the induction treatment.
D001853:Bone Marrow / Q000187:drug effects; D002478:Cells, Cultured; D003561:Cytarabine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy / Q000473:pathology
D003561:Cytarabine