Isolation of Clq-binding virus-antibody immune complexes from lactic dehydrogenase virus (LDV)-infected mice.
A Sepharose-IgG-Clq sorbent has been successfully used to isolate circulating virus-antibody (virus Ab) complexes from serum of lactic dehydrogenase virus (LDV)-infected mice. The chronological study demonstrated that although circulating LDV in persistently infected mice was complexed with IgG antibody, the virus-Ab complexes bound to the Clq sorbent only early during the infection. Inactivation of these Clq-isolated virus Ab complexes was accomplished with rabbit anti-mouse IgG but not anti-IgM. The critical time period for the demonstration of Clq-binding LDV-Ab complexes in serum was between 10 and 18 days postinoculation. The reasons for the apparent shift from Clq-binding virus-Ab complexes to non-Clq binding are not clear, however, possible explanations and implications in immune complex tissue injury are discussed.
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D003166:Complement Activating Enzymes / Q000276:immunology*; D015922:Complement C1q; D007074:Immunoglobulin G / Q000276:immunology; D007772:Lactate dehydrogenase-elevating virus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013997:Time Factors; D014777:Virus Diseases / Q000276:immunology*
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D015922:Complement C1q; D003166:Complement Activating Enzymes