Long-term maintenance of "cloned" human PLT cells in TCGF with LCL cells as a feeder layer.
The long-term maintenance of T cells "cloned" by limiting dilution in TCGF was enhanced by the use of irradiated autologous lymphoblastoid cell line (LCL) cells as well as irradiated LCL cells of the individual to which the T cells were originally primed. It was possible to obtain more than 1 X 10(12) cells from a "clone" seeded at one cell per well. Some of the clones tested express primed LD-typing activity.
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells; D003470:Culture Media; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008222:Lymphokines / Q000494:pharmacology*; D008722:Methods; D013601:T-Lymphocytes / Q000502:physiology* / Q000528:radiation effects
D003470:Culture Media; D007376:Interleukin-2; D008222:Lymphokines