Production of human antithyroglobulin in vitro, IV. Specific stimulation by antigen.
Peripheral blood lymphocytes from patients whose serum contains antithyroglobulin are capable of producing antithyroglobulin (anti-Tg) in vitro when stimulated with insolubilized Tg and suboptimal amounts of pokeweed mitogen. Antigen stimulation of anti-Tg production was demonstrated in 6 of 10 experiments in which a 1:10,000 dilution of pokeweed mitogen was also included. Larger concentrations of antigen appeared to inhibit anti-Tg synthesis. Regulation of antigen-stimulated anti-Tg production by the patients' T cells was not different from regulation by the T cells of normal subjects. Both T help and T suppression of antigen-induced antibody synthesis was demonstrated with patients' T cells. These experiments continue to provide evidence that production of the autoantibody anti-Tg is related to an abnormality of B cells.
D000328:Adult; D001327:Autoimmune Diseases / Q000276:immunology*; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011043:Pokeweed Mitogens / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D013954:Thyroglobulin / Q000276:immunology*
D007074:Immunoglobulin G; D011043:Pokeweed Mitogens; D013954:Thyroglobulin