Binding of peanut lectin to thymic cortex and germinal centres of lymphoid tissue.
The binding pattern of horseradish peroxidase conjugated peanut lectin (HRP-PNL) on frozen sections of lymphoid tissue from man, mouse, rat, hamster, guinea-pig, rabbit, sheep and chicken has been investigated. Binding of PNL was found to be highly species dependent; man, mouse and sheep showed strong binding to lymphocytes in thymic cortex and germinal centres; lymphoid tissue from hamster, guinea-pig and rabbit did not stain with HRP-PNL and rat showed only lightly positive cells in thymic cortex and germinal centres; all lymphoid tissue from chicken, except the bursal cortex, bound PNL. Neuraminidase treatment of tissues which did not bind PNL resulted in strongly PNL-positive cells. Double binding studies on murine Peyer's patches with fluorescein isothiocyanate conjugated PNL (FITC-PNL) and tetramethylrhodamine isothiocyanate (TRITC)-anti-Thy-1.2 or anti-immunoglobulin reagents revealed 3%-10% of PNL positive cells to be Thy-1.2 positive and 70%-80% to bear surface immunoglobulin.
D000371:Agglutination; D000818:Animals; D010367:Arachis / Q000276:immunology; D001402:B-Lymphocytes / Q000187:drug effects; D002645:Chickens; D006224:Cricetinae; D006168:Guinea Pigs; D006801:Humans; D037102:Lectins / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology*; D008297:Male; D051379:Mice; D009439:Neuraminidase / Q000494:pharmacology; D037121:Plant Lectins; D011817:Rabbits; D051381:Rats; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D012756:Sheep; D013950:Thymus Gland / Q000276:immunology*
D037102:Lectins; D037121:Plant Lectins; D011947:Receptors, Antigen, B-Cell; D009439:Neuraminidase