A simple preparation, for microscopical investigations, of monolayered cells at specific cell-cycle stages.
A technique is described whereby cells at the mitotic stage are washed off into specially designed culture tubes. Because these tubes have a small culturing area in relation to the volume of medium they contain, and the area of the original culture flask, a usable number of cell-cycle synchronized cells are obtainable for microscopic study.
D000818:Animals; D002453:Cell Cycle; D002478:Cells, Cultured / Q000166:cytology*; D002999:Clone Cells / Q000166:cytology; D003239:Connective Tissue Cells; D003584:Cytological Techniques; D007440:Intubation / Q000295:instrumentation; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008938:Mitosis