title: Lymphoid source and target of murine leukocyte adherence inhibition factor (LAIF).
abstract: The cellular source of murine LAIF detected by an indirect leukocyte adherence inhibition (LAI) assay using capillary tubes was investigated using non-inducing peritoneal cells (PC) from normal and spindle-cell sarcoma-bearing A/J mice. Cell populations containing greater than 95% T-cells, B-cells or macrophages were prepared from PC using a series of elimination and enrichment procedures. The in vitro incubation of enriched T-cell populations (less than 0.05% macrophages) from tumor-bearing mice with the specific soluble tumor antigen did not result in the release of detectable levels of LAIF; however, the addition of 10% normal isologous macrophages to the T-cells resulted in a significant release of LAIF. Enriched B-cell populations did not release LAIF either by themselves of when 10% normal isologous macrophages were added. Cell populations containing both T-cells and B-cells produced significant levels of LAIF, but only when suitable numbers of macrophages were present. Treatment with anti-Thy-1.2 alloantiserum and complement resulted in the abrogation of LAIF production by mixed cell populations. Using Lyt-1.2 and Lyt-2.2 alloantisera and complement to prepare either Lyt-1.2 or Lyt-2.2 depleted T-cell populations, it was found that the Lyt-1.2 subpopulation was responsible for the release of LAIF in this test system. LAIF was found to be effective in reducing the glass adherence of macrophages but not of T-cells or B-cells.
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm; D001402:B-Lymphocytes; D002448:Cell Adhesion; D066298:In Vitro Techniques; D007957:Leukocyte Adherence Inhibition Test; D008222:Lymphokines / Q000096:biosynthesis* / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D012513:Sarcoma, Experimental / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm; D008222:Lymphokines