Inhibition of active transport of chloride and sodium by vanadate in the cornea.
Vanadate, an inhibitor of (Na+ + K+)ATPase, also inhibits active transepithelial Na+ and Cl- transport (measured as short-circuit current) in the isolated cornea in concentrations of 10(-4)M to 10(-3)M. The disulfonic stilbene, DIDS, does not affect the short-circuit current per se, but it prevents the inhibitory effect of vanadate on Na+ and Cl- transport. Since vanadate acts in other systems from the cytoplasmic side, it is postulated that DIDS interferes with vanadate penetration into the epithelial cells. These results also strengthen the notion of a Na-Cl co-transport system in the corneal epithelium.
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002712:Chlorides / Q000378:metabolism*; D003315:Cornea / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D011892:Rana catesbeiana; D012964:Sodium / Q000378:metabolism*; D014638:Vanadates; D014639:Vanadium / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D002712:Chlorides; D014639:Vanadium; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D014638:Vanadates; D012964:Sodium; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid