Characterization of androgen uptake by purified nuclei from an androgen-dependent and two androgen-independent cell lines of the Shionogi mouse mammary carcinoma.
Properties of androgen uptake by nuclei prepared from an androgen-dependent tumour cell line occurred after 2 h incubation at 20 degrees C; 2. Nuclei from three tumour cell lines displayed similar affinity for DHT but the two androgen-independent cell lines had less than one-quarter the number of uptake sites; 3. Loss of label from nuclei which had been pre-incubated with [3H]-DHT for 18 h at 20 degrees C was greater from the AD cell line nuclei than from the androgen-independent cell lines; 4. Whole cell contamination of the nuclear preparations did not contribute to specific DHT uptake.
D000728:Androgens / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism; D004963:Estrenes / Q000378:metabolism; D005260:Female; D007700:Kinetics; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism*; D015741:Metribolone; D051379:Mice; D012737:Sex Factors; D045165:Testosterone Congeners / Q000378:metabolism
D000728:Androgens; D004963:Estrenes; D045165:Testosterone Congeners; D013196:Dihydrotestosterone; D015741:Metribolone