Growth hormone stimulation of normal and leukemic human T-lymphocyte proliferation in vitro.
In order to investigate the effect of human growth hormone on T lymphocytes, we utilized a clonogenic assay for mitogen-responsive human peripheral blood T lymphocytes. Lymphocytes were purified by density gradient centrifugation and incubated in the presence of phytohemagglutinin using a two-layer agar technique for CFU- T-lymphocyte culture. Nanogram concentrations of human growth hormone, ovine prolactin, human chorionic somatomammotropin, or growth hormone fragment were added to cell cultures. Growth hormone potentiated normal T-cell colony formation in a species-specific manner. Cells from a homogeneous T-lymphoblast line derived from a patient with a T-cell variant of hairy cell leukemia also showed augmentation of colony growth in the presence of human growth hormone. These studies provide evidence for a direct effect of growth hormone on normal and some neoplastic human T cells.
D000818:Animals; D002417:Cattle; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells / Q000166:cytology; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008213:Lymphocyte Activation; D011388:Prolactin / Q000494:pharmacology; D012756:Sheep; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D006133:Growth Substances; D011388:Prolactin