Radioimmunoassay of CRF-like material in rat hypothalamus.
Corticotropin releasing factor (CRF) was recently isolated from ovine hypothalami by its ability to stimulate adrenocorticotropin (ACTH) and beta-endorphin release from dispersed rat pituitary cells. In order to study the physiology of this peptide, we have developed a sensitive and specific radioimmunoassay (RIA) for CRF. Synthetic CRF was conjugated to bovine thyroglobulin and emulsified with complete Freund's adjuvant. A suitable antiserum was obtained which showed no crossreactivity with eight naturally occurring peptides. N-Tyr-CRF was iodinated and used as tracer. With this assay, CRF-like immunoreactivity which coeluted with ovine CRF on Sephadex G50 was detected in rat hypothalami.
D000818:Animals; D000918:Antibody Specificity; D003346:Corticotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000032:analysis* / Q000276:immunology; D007031:Hypothalamus / Q000032:analysis*; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D008297:Male; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains
D007457:Iodine Radioisotopes; D003346:Corticotropin-Releasing Hormone