Effect of age on glucose oxidation by isolated rat islets.
Islets were isolated from pancreases of 2-month and 12-month-old rats, and the oxidation of 14C-glucose to 14CO2 determined at various medium D-glucose concentration. Islets from 12-month-old rats oxidized significantly less glucose than those from 2-month-old rats at glucose concentrations of 150, 300, and 450 mg/dl, and this was true when islets were selected by hand or by Ficoll density gradient separation. The effect of age on glucose oxidation was seen when islets were incubated with [U-14C], [1-14C], or [6-14C] glucose. The results raise the possibility that previously reported age-related defects in glucose-stimulated insulin secretion may be secondary to the effect of age on islet glucose catabolism.
D000375:Aging; D000818:Animals; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D007515:Islets of Langerhans / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D051381:Rats
D005947:Glucose