Bromouracil-induced mutagenesis in a mismatch-repair-deficient strain of Haemophilus influenzae.
Cells of wild-type Haemophilus influenzae and of a mismatch-repair-deficient mutant (hex-) were grown in a chemically defined medium containing either thymidine or 5-bromodeoxyuridine (BUdR). Spontaneous mutation frequencies to resistance against 3 antibiotics observed for the thymidine cultures were 10-30 times higher for the hex- mutant. The mutation frequencies observed for the BUdR hex- culture were increased by another 10 times while those for the wild-type suspension did not differ from the frequencies seen in the thymidine medium.
D000900:Anti-Bacterial Agents / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D011088:DNA Ligases / Q000172:deficiency; D004260:DNA Repair / Q000187:drug effects; D004279:DNA, Viral; D004352:Drug Resistance, Microbial / Q000187:drug effects; D006193:Haemophilus influenzae / Q000187:drug effects / Q000235:genetics*; D009154:Mutation
D000900:Anti-Bacterial Agents; D004279:DNA, Viral; D011088:DNA Ligases; D001973:Bromodeoxyuridine