Enzyme therapy and immuno-adsorption by an extra-corporeal device.
An extra-corporeal chamber has been constructed and used for diagnosis, enzyme therapy and immuno-adsorption. The chamber is made from polymethyl-acrylate and the dimensions of the chamber are 10 x 18 x 7 cm. It contains 23 plates with a total area of 0.5 m2. In most cases no pump is used. The active substance is bound covalently to the plate surfaces. The time for each hemoperfusion has been 3-6 hr. No infections,  decrease of thrombocytes or other side effects have been observed. By immobilized homologous antisera (F (ab')2), three putative tumour-associated antigens were isolated from cases of hypernephroma. Two children and one adult patient with malignant lymphoma were treated with extra-corporeal L-asparaginase, the latter with repeated remissions of metastases. The level of antibodies against Factor VIII was significantly decreased by chamber immunoadsorption in two cases of hemophilia. A number of kidney transplantation cases received extra-corporeal L-asparaginase pre- and post-operatively to counteract rejection. In all around 50 patients have received extra-corporeal treatment without any adverse effects. The use of the extra-corporeal chamber in other diseases is discussed.
D000230:Adenocarcinoma / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000951:Antigens, Neoplasm / Q000302:isolation & purification; D001215:Asparaginase / Q000627:therapeutic use; D001672:Biocompatible Materials; D002648:Child; D004285:Dogs; D057487:Enzyme Therapy; D005112:Extracorporeal Circulation / Q000295:instrumentation*; D005167:Factor VII / Q000276:immunology; D006464:Hemoperfusion / Q000295:instrumentation; D006689:Hodgkin Disease / Q000188:drug therapy; D006801:Humans; D007163:Immunosorbent Techniques / Q000295:instrumentation*; D016030:Kidney Transplantation; D008545:Melanoma / Q000276:immunology; D008768:Methylmethacrylates; D011108:Polymers; D014184:Transplantation, Homologous
D000906:Antibodies; D000951:Antigens, Neoplasm; D001672:Biocompatible Materials; D008768:Methylmethacrylates; D011108:Polymers; D005167:Factor VII; D001215:Asparaginase