The estimation of B lymphocytes and their subpopulations in pigs. A comparison between a method using whole blood and one using Ficoll-Paque purified mononuclear cells.
This paper describes a simple method for determining the proportion of B lymphocytes and their subtypes in pigs, using washed whole blood. The method was compared with one using Ficoll-Paque purified mononuclear cells and was found to give significantly lower results (13.0 +/- 1.3% and 17.0 +/- 1.8%, mean +/- S.E., respectively). For 30 normal pigs, 13.9 +/- 6% (mean +/- S.D.) of lymphocytes in whole blood bore light chains and 12.1 +/- 5% bore IgM, while of lymphocytes in purified mononuclear cells, 17.0 +/- 6% bore light chains, 15.1 +/- 6% bore IgM, 1.4 +/- 1.4% bore IgG and 0.5 +/- 0.5% bore IgA.
D000818:Animals; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D005260:Female; D007958:Leukocyte Count / Q000379:methods; D010586:Phagocytes; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell; D013552:Swine
D011947:Receptors, Antigen, B-Cell