Identification of idiotypic receptors on reovirus-specific cytolytic T cells.
Cytolytic T lymphocytes (Tc) specific for cells infected with reovirus type 3 were shown to lyse an uninfected B-cell hybridoma line (designated 87.92.6). This hybridoma expresses and secretes an anti-idiotypic antibody that reacts with a monoclonal antibody (termed G-5). G-5 recognizes a domain on the hemagglutinin of the reovirus that is relevant to virus tropism. The Tc cell response was H-2 restricted and could be inhibited by G-5. As shown by limiting dilution analysis, identical clones lysed reovirus-infected and anti-idiotype-bearing target cells. Tc cells induced by a variant of wild-type reovirus type 3 (immunologically selected by resistance to neutralization by G-5) were unable to recognize the anti-idiotype-bearing cells although they lysed reovirus type 3-infected tumor cells. We conclude that Tc cells must bear determinants that bind anti-idiotype molecules and, furthermore, that B cells and cytolytic T cells can share these idiotypic determinants.
D000818:Animals; D000914:Antibodies, Viral; D000954:Antigens, Surface / Q000276:immunology; D002460:Cell Line; D006183:H-2 Antigens / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007130:Immunoglobulin Idiotypes; D012089:Mammalian orthoreovirus 3 / Q000276:immunology*; D051379:Mice; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D011971:Receptors, Immunologic / Q000276:immunology*; D012087:Reoviridae / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000914:Antibodies, Viral; D000954:Antigens, Surface; D006183:H-2 Antigens; D007130:Immunoglobulin Idiotypes; D011948:Receptors, Antigen, T-Cell; D011971:Receptors, Immunologic