Nonrandom chromosomal aberrations in chronic lymphocytic leukemia revealed by polyclonal B-cell-mitogen stimulation.
Peripheral blood lymphocytes from 11 patients with chronic lymphocytic leukemia were stimulated by Epstein-Barr virus (EBV), lipopolysaccharide from Escherichia (LPS), and phytohemagglutinin (PHA). Chromosome analysis with the Q-banding technique after 5 days incubation revealed an extra chromosome 12 in 5 of the patients and a translocation between chromosome 11 and chromosome 14 in 1. Two patients had a deletion of chromosome 6, and only 3 patients had a normal karyotype. In most patients, the abnormalities were found in the majority of metaphases after stimulation with EBV, LPS, or both mitogens, while PHA revealed a normal karyotype, with the exception of a total of 4 metaphases in 3 patients. An extra chromosome 12 appears to be specifically associated with chronic lymphocytic leukemia. The frequency of chromosomal abnormalities in this disease appears to be much higher than has previously been thought.
D001402:B-Lymphocytes / Q000276:immunology* / Q000503:physiopathology; D002869:Chromosome Aberrations; D002906:Chromosomes, Human, 6-12 and X; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007945:Leukemia, Lymphoid / Q000235:genetics* / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D008677:Metaphase; D008934:Mitogens / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000276:immunology
D008934:Mitogens; D011947:Receptors, Antigen, B-Cell; D004247:DNA