Hydroxyurea increases in vitro, antigen-independent B cell development in bone marrow.
The fluorescence-activated cell sorter and Coulter counter were used to study the effect of hydroxyurea in vitro in B cell development in 24 hr cultures of adult mouse bone marrow. This agent, which kills cells in the S phase of the cell cycle, caused a 40-70% increase in the absolute number of B cells in bone marrow cultures as compared with untreated cultures. There was also an increased response to the B cell mitogen, bacterial lipopolysaccharide, as measured by the stimulation index for 3H-thymidine incorporation. Hydroxyurea in vitro increased B cells numbers in fetal liver which contains pre-B cells, but not in lymph nodes or spleen which lack these cells.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects*; D001853:Bone Marrow / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D006918:Hydroxyurea / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000196:embryology; D008198:Lymph Nodes / Q000187:drug effects; D008213:Lymphocyte Activation; D051379:Mice; D008808:Mice, Inbred CBA; D008938:Mitosis; D013154:Spleen / Q000187:drug effects
D008070:Lipopolysaccharides; D006918:Hydroxyurea