Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein.
The nonstructural 100,000-dalton (100K) protein of type 5 adenovirus was isolated and purified from infected KB cells by a combination of ion-exchange and affinity chromatographies. Rabbit antiserum containing specific 100K protein antibodies was used for indirect immunofluorescence examination of cells infected with wild-type virus, 100K mutants, and hexon mutants. The 100K protein, which is synthesized as a late protein, was observed primarily in the cytoplasm of cells infected with wild-type and mutant viruses.
D000260:Adenoviruses, Human / Q000032:analysis* / Q000254:growth & development / Q000276:immunology; D000914:Antibodies, Viral; D003593:Cytoplasm / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D011233:Precipitin Tests; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*
D000914:Antibodies, Viral; D014764:Viral Proteins