Dissimilar modes of expression of beta- and gamma-actin in normal and leukemic human T lymphocytes.
Actin polypeptides are the most abundant protein components of replicating normal human T lymphocytes, comprising 16% of the total cellular protein. Unlike other replicating cell types which synthesize equal proportions of beta- and gamma-actin polypeptides, T cells synthesize beta-actin as the predominant species. By contrast, leukemic T cells (the Molt-4 cell line) synthesize nearly equal proportions of beta- and gamma-actin. In addition, novel forms of beta-actin in normal T cells and of beta- and gamma-actin in Molt-4 cells appear to be present, perhaps resulting from a post-translational modification process. Otherwise, both divergent T cell types express a relatively common spectrum of polypeptide species, thereby suggesting that regulation of actin biosynthesis may be involved in the neoplastic transformation process.
D000199:Actins / Q000096:biosynthesis*; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D007945:Leukemia, Lymphoid / Q000378:metabolism*; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D013601:T-Lymphocytes / Q000378:metabolism*
D000199:Actins