[Semicontinuous cultivation of fungi of the genus Aspergillus, producers of hydrolases].
The production of exohydrolases (alpha-amylase and pectinase) by fungi belonging to the genus Aspergillus was studied in the course of batch cultivation and, if immobilized cells were used, in the semicontinuous regime of growth. The cells were immobilized on a fixed filtering plate and on floating, in the growth medium, polyhedrons. Such a cultivation of immobilized microbial cells in the semicontinuous regime of growth on submerged polyhedrons freely floating in the nutrient medium makes it possible to cultivate the cells for 1.5 months with the active production of exocellular hydrolases. Under these conditions, Aspergillus oryzae 3-9-15 produces more alpha-amylase and A. awamori synthesizes more pectinases.
D001230:Aspergillus / Q000201:enzymology* / Q000254:growth & development; D001236:Aspergillus oryzae / Q000201:enzymology / Q000254:growth & development; D003470:Culture Media / Q000378:metabolism; D006867:Hydrolases / Q000096:biosynthesis*; D011096:Polygalacturonase / Q000096:biosynthesis; D013997:Time Factors; D000516:alpha-Amylases / Q000096:biosynthesis
D003470:Culture Media; D006867:Hydrolases; D000516:alpha-Amylases; D011096:Polygalacturonase
Polunepreryvnoe kul'tivirovanie gribov roda Aspergillus--produtsentov gidrolaz.