Mitogenic effects of partially purified interleukin 2 on thymocyte subpopulations and spleen t cells of the mouse.
Partially purified interleukin 2 (IL-2) promotes proliferation of mouse spleen T, but not B cells, and of peanut-agglutinin-negative (PNA-), and cortisone-resistant "mature" thymocytes, but not of PNA+ "immature" thymocytes. Within the cortisone-resistant thymocyte population, IL-2-responsive cells were found in the blast cell fraction. Proliferation was measured by [3H] thymidine incorporation and subsequent increase in viable cells. The mitogenic effect of IL-2 could not be inhibited by 50 mM methyl-alpha-D-mannoside which excludes contaminating concanavalin A (Con A) as a cause of mitogenicity. The relative increase in viable cells in IL-2 vs. control cultures was abrogated by 1.5 mM hydroxyurea. A possible effect of IL-2 on cell survival is thus ruled out. IL-2, when acting as comitogen with Con A, affected only PNA- and cortisone-resistant thymocytes. These cells also showed high intrinsic IL-2 release when stimulated with Con A such that a comitogenic effect of externally added IL-2 was only seen at low cell concentrations. PNA+ thymocytes could neither be induced to release IL-2 nor did these cells become Con A-responsive under the influence of IL-2, thereby excluding an IL-2-mediated maturation.
D000373:Agglutinins / Q000276:immunology; D000818:Animals; D010367:Arachis; D001402:B-Lymphocytes / Q000276:immunology; D002469:Cell Separation; D002470:Cell Survival; D007376:Interleukin-2 / Q000302:isolation & purification* / Q000494:pharmacology; D008222:Lymphokines / Q000302:isolation & purification*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008934:Mitogens / Q000494:pharmacology*; D011951:Receptors, Complement; D013025:Glycine max; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology; D013936:Thymidine / Q000378:metabolism
D000373:Agglutinins; D007376:Interleukin-2; D008222:Lymphokines; D008934:Mitogens; D011951:Receptors, Complement; D013936:Thymidine