Induction of mutations in specific genes of bacteriophage T4 using cloned restriction fragments and marker rescue.
Saturation of a specific region of a chromosome with conditional lethal mutations becomes increasingly difficult as the genome size of the organism increases. We show that mutagenesis of cloned genes followed by their re-integration into a non-mutagenized organism is a practical way to circumvent this difficulty.
D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005804:Genes, Lethal; D005814:Genes, Viral; D006898:Hydroxylamines / Q000494:pharmacology; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines / Q000494:pharmacology; D011995:Recombination, Genetic; D013604:T-Phages / Q000235:genetics*
D004274:DNA, Recombinant; D006898:Hydroxylamines; D009153:Mutagens; D009604:Nitrosoguanidines