title: Effects of anesthesia, surgical manipulation and dehydration on the nucleic acid and protein content of the pituitary and hypothalamus of the frog.
abstract: Various types of stress, chemical or surgical, have a negative influence on the protein and RNA content of the hypothalamo-hypophyseal system of Rana esculenta. A degree of recovery occurs in these tissues 24 h after MS222 anaesthesia and laparotomy. Decapitation is apparently the most suitable method of sacrificing the animals.
mesh_terms: D000758:Anesthesia; D000818:Animals; D003681:Dehydration / Q000378:metabolism; D007031:Hypothalamus / Q000032:analysis*; D008297:Male; D009696:Nucleic Acids / Q000032:analysis*; D010902:Pituitary Gland / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D011893:Rana esculenta; D013048:Specimen Handling; D013312:Stress, Physiological / Q000378:metabolism; D013514:Surgical Procedures, Operative
chemical_list: D009696:Nucleic Acids; D011506:Proteins