A mitogen for human B cells: anti-Ig coupled to polyacrylamide beads activates blood mononuclear cells independently of T cells.
The F(ab')2 fragment of rabbit anti-human F(ab')2 antibodies covalently linked to polyacrylamide beads (anti-Ig beads) acts as a polyclonal mitogen for human B cells in whole peripheral blood mononuclear cells (PBM) or tonsil cells. Activation, measured by the rate of DNA synthesis, is the result of an interaction between the anti-Ig beads and membrane immunoglobulin found on B cells. Excess soluble human F(ab')2 blocks activation with anti-Ig beads. Immunospecifically purified antibodies against IgM or IgD bound to beads activate lymphocytes. Also soluble anti-IgM or anti-IgD can block activation with insolubilized anti-IgM or anti-IgD, respectively, by modulating membrane immunoglobulin. Irrelevant rabbit antihapten antibodies bound to beads failed to activate PBM or tonsil cells. Anti-Ig beads act independently of T cells. The anti-Ig beads lead to activation of B cells without further maturation to polyclonal immunoglobulin secretion.
D000180:Acrylic Resins / Q000494:pharmacology; D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000166:cytology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation; D006801:Humans; D007072:Immunoglobulin D / Q000276:immunology; D007140:Immunoglobulin Fab Fragments; D007075:Immunoglobulin M / Q000276:immunology; D008213:Lymphocyte Activation; D008863:Microspheres; D008934:Mitogens / Q000494:pharmacology*; D014066:Palatine Tonsil / Q000166:cytology; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000888:Antibodies, Anti-Idiotypic; D007072:Immunoglobulin D; D007140:Immunoglobulin Fab Fragments; D007075:Immunoglobulin M; D008934:Mitogens; D000099166:Polyacrylamides