A PMN-derived factor that enhances DNA-synthesis in PHA or antigen-stimulated lymphocytes.
The DNA synthesis potentiation of a mouse polymorphonuclear leukocyte (PMN) factor was studied by using phytohemagglutinin (PHA)-stimulated syngeneic thymocytes. The partially purified PMN factor was nonmitogenic and exhibited maximum potentiation of thymocytes when added within 3 hr of PHA stimulation. PHA-stimulated, but not nonstimulated thymocytes absorbed PMN potentiation factor. 125I-labeled factor binding to stimulated thymocytes was observed. Our results suggest that an acceptor site for PMN factor may be generated on the thymocyte surface only after PHA stimulation.
D000042:Absorption; D000818:Animals; D000941:Antigens; D001665:Binding Sites; D002448:Cell Adhesion; D002457:Cell Extracts / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009504:Neutrophils / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors; D014020:Tissue Extracts / Q000494:pharmacology*
D000941:Antigens; D002457:Cell Extracts; D010835:Phytohemagglutinins; D014020:Tissue Extracts; D004247:DNA