Analysis of T-lymphocyte subpopulations in juvenile-onset diabetics.
In juvenile-onset diabetics (JOB) subpopulations of T-lymphocytes in the peripheral blood have been analysed by the E-rosetting technique. We determined the number of 'low', 'intermediate' and 'high affinity' ('active') rosette forming T-cells by the use of three different lymphocyte:sheep red blood cell ratios in the assay. When comparing the percentage and absolute number of peripheral T-cells between twenty well controlled JODs and twenty normal persons a slight reduction was found in diabetics. Of the three T-lymphocyte subpopulations only active rosette forming cells appeared to be affected. We conclude that minor differences exist in number and characteristics of peripheral blood T-cells between well controlled JODs and normal persons.
D000293:Adolescent; D000328:Adult; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D004906:Erythrocyte Count; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology