[Isolation of hybrid clones of human-rodent somatic cells].
Mutant cells of Chinese hamster or mouse were fused with normal embryonic human cells. The fusion was induced by polyethylene glycol. The Eagle medium containing hypoxanthine, aminopterine, thymidine, glycine, and ouabain served as a selective medium. The parent cells were completely destroyed in the selective medium. The hybrid cells formed colonies which were isolated and reproduced for cytogenetic analysis. The cytogenetic analysis has confirmed the hybrid origin of the isolated clones.
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003470:Culture Media; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology* / Q000648:ultrastructure; D007621:Karyotyping; D051379:Mice; D010042:Ouabain / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology
D003470:Culture Media; D005819:Genetic Markers; D011092:Polyethylene Glycols; D010042:Ouabain
Poluchenie gibridnykh klonov somaticheskikh kletok chelovek-gryzun.