Enhanced clonability of human T lymphocytes caused by their culturing in vitro.
Human lymphocytes precultured for 24--48 hr form more T-lymphoid colonies (LT-CFU) in agar than do fresh cells. It appears that during the preculture period lymphocytes proceed from G0 to the next S phase, a process that is dependent upon active cellular metabolism. Thus, lymphoproliferation as assayed by LT-CFU growth appears to be controlled by short-lived suppressor cells (SLSC) which may be similar to those described for blastogenesis evaluated by thymidine incorporation assay. The activity of SLSC operating in LT-CFU system may be controlled by prostaglandins.
D000362:Agar; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D015232:Dinoprostone; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007399:Interphase; D008213:Lymphocyte Activation / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D010835:Phytohemagglutinins; D011458:Prostaglandins E; D000362:Agar; D015232:Dinoprostone; D007213:Indomethacin