title: Variables in demonstrating methicillin tolerance in Staphylococcus aureus strains.
abstract: Certain technical considerations which affected the status of methicillin tolerance in Staphylococcus aureus strains were studied. Methods which consistently demonstrated tolerance or intolerance of a given strain were avoidance of inoculum splashing, use of stationary-phase inoculum, 24-h tube incubation, and minimization of antibiotic carry-over. These studies suggested a need for the establishment of a standardized reference for the determination of tolerance.
mesh_terms: D008712:Methicillin / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010403:Penicillin Resistance; D010405:Penicillinase / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects*; D013997:Time Factors
chemical_list: D010405:Penicillinase; D008712:Methicillin