Nitrous acid damage to duplex deoxyribonucleic acid: distinction between deamination of cytosine residues and a novel mutational lesion.
The rate of nitrous acid deamination of labeled cytosine residues in native Escherichia coli deoxyribonucleic acid was monitored in vitro by release of acid-soluble counts after treatment with uracil deoxyribonucleic acid glycosylase. The reaction exhibited a lag and was not stimulate by several agents previously shown to enhance base substitution mutagenesis during nitrous acid treatment of duplex deoxyribonucleic acid. We conclude that a significant proportion of nitrous acid induced mutagenic lesions are novel lesions and not cytosine deaminations.
D003596:Cytosine / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism*; D003641:Deamination; D004926:Escherichia coli; D007700:Kinetics; D009154:Mutation; D009573:Nitrites / Q000494:pharmacology*; D009608:Nitrous Acid / Q000494:pharmacology*; D009691:Nucleic Acid Denaturation
D004269:DNA, Bacterial; D009573:Nitrites; D003596:Cytosine; D009608:Nitrous Acid