Cytotoxicity of temporarily adherent human mononuclear blood cells. 2. Characterization of effector cells.
In monolayers of freshly isolated human mononuclear blood cells, 5-10% of the adherent cells are non-monocytic. These cells detach during the first day of in vitro culture. These detached cells exert high cytolytic and cytostatic activities. The detached cells were found to be mostly (90%) nonmonocytic as assessed by cell morphology studies and characterization with respect to membrane markers. The detached cells consisted of 40% T-lymphocytes. 10% B-lymphocytes and 40% non-T, non-B lymphocytes. Thirty-five percent of the cells were Fc-receptor-bearing cells. Following sheep red blood cell (SRBC) rosetting, the cytotoxic cells were found in the non-SRBC-rosetting fraction, viz. they were non-T lymphocytes.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002448:Cell Adhesion; D002469:Cell Separation; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007942:Leukemia, Experimental / Q000276:immunology; D007962:Leukocytes / Q000145:classification / Q000276:immunology*; D008214:Lymphocytes / Q000145:classification / Q000276:immunology / Q000648:ultrastructure; D011947:Receptors, Antigen, B-Cell; D011961:Receptors, Fc; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000201:enzymology / Q000276:immunology; D013997:Time Factors
D011947:Receptors, Antigen, B-Cell; D011961:Receptors, Fc