Regulatory role of humoral factors released by macrophages in reactivity of rat lymphocytes in vitro. III. Humoral factors released by lymph node macrophages regulating lymphocyte response to PHA.
Lymph node macrophages, derived from normal rats, generated in vitro factors enhancing or suppressing syngeneic lymph node lymphocyte stimulation in response to PHA and reconstituting the PHA-dependent response of lymphocytes deprived of native macrophages. The occurrence of one of the opposite activities depended on the degree of lymphocyte stimulation by PHA. Moreover, the expression of suppressing or stimulating effect no lymphocyte response to PHA of the same supernatants, changed twice with their dilution, (suppression leads to enhancement leads to suppression) suggesting the existence of two distinct factors: suppressing and enhancing.
D000276:Adjuvants, Immunologic; D000818:Animals; D002478:Cells, Cultured; D007166:Immunosuppressive Agents; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000276:immunology*; D008222:Lymphokines / Q000276:immunology*; D008264:Macrophages / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology; D051381:Rats; D013268:Stimulation, Chemical
D000276:Adjuvants, Immunologic; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D008222:Lymphokines; D010835:Phytohemagglutinins