Cell-mediated immunity to mouse adenovirus infection. Blocking of macrophage migration inhibition and T cell-mediated cytolysis of infected cells by anti-S antigen or anti-alloantigen serum.
Migration of peritoneal exudate cells (PEC), which were prepared from mice immunized against mouse adenovirus (M-Ad), was inhibited upon exposure to the antigenic extract of M-Ad-infected cells. This inhibition was shown to be blocked when infected cells or their extracts were pretreated with antiserum against M-Ad-induced cell surface (S) antigen(s) or with antisera against alloantigens of infected cells. Immune spleen cell-mediated cytolysis of M-Ad-infected cells was also blocked in the presence of anti-S, anti-alloantigen or anti-beta 2m serum. Immunofluorescent antibody staining of S antigen(s) was blocked when infected cells were pretreated with anti-alloantigen or anti-beta 2m serum, whereas it was not blocked when they were pretreated with anti-mouse immunoglobulin or anti-Thy-1.2 serum. Conversely, immunofluorescent antibody staining of alloantigens was blocked when infected cells were pretreated with anti-S serum. These findings indicate that S and alloantigens are associated with each other or at least located very close to each other.
D000256:Adenoviridae / Q000276:immunology*; D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D002464:Cell Migration Inhibition; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D007106:Immune Sera; D007111:Immunity, Cellular; D007519:Isoantigens / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology
D000954:Antigens, Surface; D007106:Immune Sera; D007519:Isoantigens