Production of colony stimulating activity in mixed mononuclear cell culture.
Culture medium was harvested after co-incubation of mononuclear cells collected and pooled from the peripheral blood of two different normal donors and was tested for colony-stimulating activity (CSA) in agar culture. With bone marrow from normal donors or peripheral blood from patients with chronic granulocytic leukaemia as sources of granulocyte-committed progenitor cells (CFU-c), such mixed mononuclear cell conditioned medium (MMC-CM) showed activity equal to that of unfractionated leucocyte feeder layers and greater than that of CSA prepared from lymphocytes stimulated by phytohaemagglutinin. The addition to plates of 2-mercaptoethanol during the preparation of MMC-CM enhanced CSA release. MMC-CM is thus a convenient source of CSA and its use may be preferable to that of feeder layers when day-to-day reproducibility is essential.
D001853:Bone Marrow / Q000378:metabolism; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000378:metabolism*; D006098:Granulocytes / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D007951:Leukemia, Myeloid / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*
D003115:Colony-Stimulating Factors