Analysis of cytotoxic effector cell populations by kinetic and monolayer adsorption techniques.
Cytolytically active lymphocytes were generated by immunizations between rat strains and evaluated by both kinetic and monolayer adsorption techniques. Kinetic analysis yields two values (Ka and Vmax), which are characteristic of interactions between individual effector and target cell populations. The Ka values this obtained correlate with the quantitative level of adsorption of CTL onto cellular monolayers, and we conclude that Ka is a measure of the binding avidity of effector and target cells.
D000818:Animals; D001665:Binding Sites; D002448:Cell Adhesion; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D007163:Immunosorbent Techniques; D007700:Kinetics; D008214:Lymphocytes / Q000276:immunology; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D013997:Time Factors