Quantitative studies of the tissue distribution of the antigens recognized by rabbit anti-rat, -dog and -human lymphocyte sera.
Rabbit anti-rat, -dog and -human ALS was analysed using quantitative absorptions and indirect 125I anti-immunoglobulin-binding assays to determine the amount of antibody directed against different types of antigen. The aim was (a) to determine what proportion of the antibodies was directed against leucocyte-specific antigens and (b) to see if the type of leucocyte-specific antigen recognized varied either with the species or the cell type used as the immunogen. The results showed that anti-thymocyte sera in all three species showed the same high degree of leucocyte specificity. Marked differences in the type of leucocyte-specific antigen recognized were found when anti-thymocyte and anti-lymph node lymphocyte sera were compared. A comparison of the leucocyte-specific antigens recognized by anti-dog  and -human thymocyte sera showed quantitative but not qualitative differences. The relevance of these results to the current failure of ALS in clinical practice and to the problems of ALS standardization are discussed.
D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000032:analysis*; D000961:Antilymphocyte Serum / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology; D004285:Dogs; D006801:Humans; D007962:Leukocytes / Q000276:immunology; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology; D014018:Tissue Distribution
D000941:Antigens; D000961:Antilymphocyte Serum