T-T cell interactions during cytotoxic T cell responses. IV. Murine lymphoid dendritic cells are powerful stimulators for helper T lymphocytes.
Enriched populations of Ia+ Fc receptor-negative dendritic cells were compared to other cell types for their stimulatory activity in primary mixed lymphocyte reactions to alloantigens and 2,4,6,-trinitrophenylated syngeneic cells. Dendritic cells were 20-100 times more effective than unfractionated splenocytes. A second cell type exhibiting strong stimulatory activity was an Ia+ Fc receptor-positive transiently adherent cell. Both types of stimulatory cells were only effective when able to produce the monokine interleukin 1. Thus glutaraldehyde-fixed cells were not stimulatory unless extraneous interleukin 1 was added. Stimulation of helper cells by either dendritic cells or Ia+ Fc receptor-positive cells resulted in the production of interleukin 2. The data are discussed in view of the recently formulated interleukin model as a minimal scheme to explain T-T cell interactions during the in vitro induction of cytotoxic T lymphocytes.
D000818:Animals; D000950:Antigens, Ly / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis; D008211:Lymphocyte Cooperation; D008214:Lymphocytes / Q000145:classification* / Q000276:immunology; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011961:Receptors, Fc; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*
D000950:Antigens, Ly; D000949:Histocompatibility Antigens Class II; D007106:Immune Sera; D007376:Interleukin-2; D011961:Receptors, Fc