T-cell cloning to detect the mutant 6-thioguanine-resistant lymphocytes present in human peripheral blood.
Rare thioguanine-resistant T lymphocytes, present in vivo in human peripheral blood, were isolated and grown in vitro as thioguanine-resistant cultured T cells. The conditions for their selection in vitro were such that thioguanine resistance had to have arisen in vivo. The mutant cells bore T-cell surface markers, maintained their thioguanine resistance in vitro in the presence or absence of selection, and were deficient in hypoxanthine-guanine phosphoribosyltransferase activity.
D000954:Antigens, Surface / Q000032:analysis; D002478:Cells, Cultured; D002999:Clone Cells; D004351:Drug Resistance; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D013866:Thioguanine / Q000494:pharmacology*
D000954:Antigens, Surface; D007041:Hypoxanthine Phosphoribosyltransferase; D013866:Thioguanine