Study on cellular events in post-thymectomy autoimmune oophoritis in mice. II. Requirement of Lyt-1 cells in normal female mice for the prevention of oophoritis.
Autoimmune oophoritis that develops in A/J mice after neonatally thymectomy (NTx) was prevented by a single intraperitoneal injection of spleen cells or thymocytes from normal adult female mice. Prevention of oophoritis was achieved when spleen cells were given within 2 wk after Tx. When spleen cells were obtained from neonatally oophorectomized mice, four times more cells were required for the prevention of oophoritis, but those from the mice oophorectomized on day 7 after birth had equivalent capacity to prevent oophoritis to those from normal female mice. The spleen cells from normal A/J mice that prevented the development of oophoritis in NTx A/J mice were Thy-1+, Lyt-1+,23-, Ia-, Qa-1-, sensitive to in vitro irradiation with 400 rad, resistant to administration of cyclophosphamide or anti-thymocyte serum, and were not eliminated by adult thymectomy. Thymocytes with oophoritis-preventing capacity were also found to be Lyt-1+,23- and TL-1,2,3-. These results seem to correlate well with the finding that the Lyt-1 subpopulation is substantially decreased in NTx mice. The results suggest that, in this post-thymectomy autoimmune oophoritis, NTx abrogates the Lyt-1 T cell subpopulation that serves as suppressive or regulatory cells over developing self-reactive cells directed toward ovarian antigens, and eventually may cause autoimmune oophoritis.
D000375:Aging; D000818:Animals; D000950:Antigens, Ly / Q000276:immunology*; D001327:Autoimmune Diseases / Q000209:etiology / Q000276:immunology* / Q000628:therapy; D003520:Cyclophosphamide / Q000494:pharmacology; D005260:Female; D007111:Immunity, Cellular / Q000187:drug effects / Q000528:radiation effects; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D009869:Oophoritis / Q000209:etiology / Q000276:immunology* / Q000628:therapy; D010641:Phenotype; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D013934:Thymectomy
D000950:Antigens, Ly; D003520:Cyclophosphamide