Alpha-naphthyl-acetate-esterase reaction for peripheral lymphocytes and monocytes of patients with systemic lupus erythematosus.
The proportion of T-lymphocytes and monocytes was estimated by the alpha-naphthyl-acetate-esterase-(ANAE)-reaction in blood smears of patients with systemic lupus erythematosus and normal controls. A parallel and significant reduction in the number of T-lymphocytes identified on the basis of both the ANAE and the E-rosette test was found in SLE. In the active periods of SLE more T-cells were identified by the enzyme histochemical study than by the E-rosette test, which points to a functional damage to the T-cells. A marked reduction in the number of the cells considered to be of the suppressor type was demonstrable by both methods in the active period. A distinct reduction in the number of monocytes was also demonstrable by ANAE in the patients with SLE, which may be one of the manifestations of deficient immunoregulation in this disease.
D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000201:enzymology; D008297:Male; D009000:Monocytes / Q000201:enzymology*; D009283:Naphthol AS D Esterase / Q000097:blood*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000201:enzymology*
D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases