title: Germinal centers and the B-cell system. VI. Migration pattern of germinal-center cells of the rabbit appendix.
abstract: The migration pattern of germinal center cells of the rabbit appendix was studied and compared with that of appendix dome cells, spleen cells, thymus cells and thoracic duct lymphocytes. To discriminate T- and B-cell migration pathways, normal or T-cell-depleted rabbits were used as donors. Cell suspensions were labeled in vitro with 3H-leucine followed by intravenous transfer. The migration of labeled cells in lymphoid organs was studied using autoradiography, particular attention being paid to the spleen of the recipient. B-cells from the appendix dome, spleen and thoracic-duct lymph migrate to primary follicles or the corona of secondary follicles via thymus-dependent areas of peripheral lymphoid organs. In contrast, a B-cell subpopulation from the germinal centers of the appendix migrates to the center of splenic primary follicles and into germinal centers. The migration of germinal center cells to splenic follicle centers is not enhanced by specific antigens. The migration properties of B-cells, possibly changing during differentiation, may be instrumental in the two types of immune reactions, i.e., plasma-cell reaction and germinal-center reaction.
mesh_terms: D000818:Animals; D001065:Appendix / Q000166:cytology*; D001402:B-Lymphocytes / Q000502:physiology*; D002465:Cell Movement; D007114:Immunization; D008198:Lymph Nodes / Q000166:cytology; D008221:Lymphoid Tissue / Q000166:cytology; D011817:Rabbits; D013154:Spleen / Q000166:cytology*; D013897:Thoracic Duct / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology