Biosilon optimal culture conditions and various research scale culture techniques.
Research scale cultivations of L-929, MRC-5 and primary C.E. cells on Biosilon have shown that the most uniform cell attachment is obtained in systems, where the relative velocity between cells and beads is close to zero. In microcarrier cultures the attachment yield is lower compared with stationary systems, but the multiplication rate is the same or higher. The Tecam ACA-system and a rolling bottle system have proved to be promising systems for research scale.
D000818:Animals; D002448:Cell Adhesion; D002452:Cell Count; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured / Q000166:cytology*; D002642:Chick Embryo; D003470:Culture Media; D003584:Cytological Techniques / Q000295:instrumentation; D006801:Humans; D051379:Mice; D011137:Polystyrenes
D003470:Culture Media; D011137:Polystyrenes