A new mutant of the yeast Saccharomyces cerevisiae defective in excision of UV-damaged sites in DNA.
An UV-sensitive yeast mutant, uvs12, with almost unchanged sensitivity to gamma-irradiation and methylmethane sulphonate was obtained. uvs12, non allelic to any of the known UV-sensitive mutants from radI to rad21 is defective in early steps of excision repair. This inference is based on the fact that after 4-5 h post-irradiation incubation unexcised pyrimidine dimers are retained in nuclear DNA, which follows from two independent tests:  the retention of UV-endonuclease-sensitive sites and enhanced survival after photoreactivation.
D004260:DNA Repair / Q000528:radiation effects; D004271:DNA, Fungal / Q000235:genetics / Q000528:radiation effects; D005720:Gamma Rays; D008027:Light; D009154:Mutation; D010641:Phenotype; D011740:Pyrimidine Dimers / Q000528:radiation effects; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014466:Ultraviolet Rays
D004271:DNA, Fungal; D011740:Pyrimidine Dimers