title: Immunological studies on the antitumor effect of sporamycin.
abstract: The role of immunological effects in the antitumor activity of sporamycin activity of sporamycin was studied mainly using the mouse syngeneic tumor, Meth-A. Sporamycin inhibited the growth of the Meth-A tumor and Balb/c mice, and 12 out of 19 mice were cured. The 12 survivors did not show any growth of reinoculated Meth-A cells. Enzymes-histochemical observations revealed marked activities of both acid phosphatase and alpha-naphthyl acetate esterase in lymphoid cells and macrophages which heavily infiltrated the tumor tissue. Pretreatment of mice with sporamycin produced a remarkable inhibition of tumor growth. A marked neutralization activity against tumor cells was observed by Winn's method using spleen cell of tumor-bearing mice which had been pre- or post-treated with sporamycin. However, this neutralization activity was depressed by anti-Thy 1.2 serum plus complement. The response of spleen cells to concanavalin A was increased by treatment with sporamycin in mice. All of these findings suggest that sporamycin may, at least partially, act by modulating the cellular immune response.
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic / Q000627:therapeutic use*; D000961:Antilymphocyte Serum / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000188:drug therapy / Q000276:immunology*; D009500:Neutralization Tests; D010455:Peptides / Q000627:therapeutic use; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D000961:Antilymphocyte Serum; D010455:Peptides; D003208:Concanavalin A; C013354:sporamycin