Direct evidence for a two-signal mechanism of cytotoxic T-lymphocyte activation.
The cellular requirements for the activation of cytotoxic T lymphocytes to alloantigens are complex. In addition to cytotoxic precursors (CLPs), metabolically active stimulator cells, adherent accessory (A) cells and antigen-specific helper T cells are also required. However, the requirement for A cells, metabolically active stimulator cells or helper T cells can be replaced by soluble factors or co-stimulator (CoS), a lymphokine obtained by stimulation of murine spleen cells with concanavalin A (Con A). We show here that in the presence of CoS, cultures containing on average one lymph node lymphocyte (LNL) and Con A can be activated to produce single cytotoxic clones. This observation strongly suggests that one of the target cells of CoS is the CLP and provides more direct evidence for a two-signal mechanism for cytotoxic T lymphocyte activation.
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D006183:H-2 Antigens; D007519:Isoantigens; D008222:Lymphokines / Q000276:immunology*; D051379:Mice; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D006183:H-2 Antigens; D007519:Isoantigens; D008222:Lymphokines; D003208:Concanavalin A