title: A solid-phase antibody binding-inhibition test, for the assay of Plasmodium berghei antigen and antibodies, using radioiodinated protein A.
abstract: Sonicated red blood cells (RBC) of rats infected with Plasmodium berghei (Pb) were used to coat plastic tubes with Pb antigens. The antigen-coated tubes were employed to detect Pb antigens and antibodies, with high efficiency. Anti-Pb antibodies were estimated by treating the tubes with rabbit or rat anti-Pb sera and assaying the bound Ig with radiolabeled Staphylococcus PrA. Pb antigens were detected by their capacity to inhibit the binding of the anti-Pb antibodies. Using a rabbit-Pb serum, sonicated, infected RBC (50% parasitemia) gave detectable inhibition up to 1 : 106 dilution.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000941:Antigens / Q000032:analysis*; D004912:Erythrocytes / Q000276:immunology / Q000469:parasitology; D008288:Malaria / Q000276:immunology / Q000469:parasitology; D010962:Plasmodium berghei / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats; D013205:Staphylococcal Protein A
chemical_list: D000906:Antibodies; D000941:Antigens; D013205:Staphylococcal Protein A