Continuous in vitro culture of human T lymphocytes.
A method for continuous culture of human T lymphocytes by stimulation with irradiated lymphocytes, lymphoblastoid cells, and phytohaemagglutinin is described. Cultures can be maintained for at least 9 months. Growth appears to be dependent on a soluble factor released by lymphocytes when they are stimulated with lymphoblastoid cells and phytohaemagglutinin.
D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods*; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000528:radiation effects; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors; D014965:X-Rays
D010835:Phytohemagglutinins