Application of mutagenicity test for milk.
Mutagenic activity in milk and milk products was tested by the Salmonella mammalian-microsome mutagenicity test (Ames test). Salmonella mutants susceptible to base-pair substitution and frame-shift mutagens gave positive results with heat-sterilized milk, particularly after activation by the S-9 fraction of microsomes. Maximum increase of His+ revertants was more than twice the spontaneous increase. The chemical specification of the mutagenic activity was not yet defined, but it was present in the chloroform extract of milk.
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002417:Cattle; D005506:Food Contamination; D006639:Histidine / Q000378:metabolism; D008892:Milk / Q000032:analysis* / Q000378:metabolism; D009153:Mutagens / Q000302:isolation & purification* / Q000378:metabolism; D012486:Salmonella typhimurium / Q000378:metabolism*
D009153:Mutagens; D006639:Histidine