title: Observations of radiation-induced chromosome fragment loss in live mammalian cells in culture, and its effect on colony-forming ability.
abstract: Our preceding paper (Crote, Joshi, Revell and Shaw 1981) described a method for the direct scrutiny of live cultured mammalian cells with a microscope, and reported that all diploid Syrian hamster cells (BHK 21 C13) of a sample given 1.4 Gy of 220 kV X-rays in Gl reached post-radiation mitosis without discernible abnormality, but then diverged in observed behaviour: descendent cells from some first mitoses continued to proliferate normally while cells from other first mitoses behaved abnormally and produced either slow-growth or stop-growth colonies. This paper completes our study of the same irradiated cell sample, and shows that these post-mitotic differences in clonogenic ability were related to acentric chromosome fragment losses at post-radiation mitosis, which were detected in live daughter-cell pairs as micronuclei. The proportion of live daughter-cell pairs scored as deficient was at least 80 per cent of the proportion of comparable fixed-and-stained mitoses with detected acentric fragments.
mesh_terms: D000818:Animals; D002478:Cells, Cultured / Q000528:radiation effects*; D002869:Chromosome Aberrations; D006224:Cricetinae; D005347:Fibroblasts / Q000528:radiation effects; D008647:Mesocricetus; D008938:Mitosis; D013997:Time Factors; D014965:X-Rays