Human B cell differentiation. II. Pokeweed mitogen-responsive B cells belong to a surface immunoglobulin D-negative subpopulation.
Surface immunoglobulin D (IgD)-positive lymphocytes precoated with monoclonal anti-delta antibody were selectively removed from blood mononuclear cell preparations by "panning" and by fluorescence-activated cell sorter. The depletion of sIgD+ cells did not significantly affect plasma cell responses to pokeweed mitogen PWM). PWM-responsive B cells lacking sIgD and mouse erythrocyte receptors preferentially sedimented in lower density fractions of a discontinuous Percoll gradient, and sIgD-negative B cells were found to have a larger mean diameter than IgD-positive cells. We conclude that PWM-responsive B cells represent a distinct subpopulation of relatively large cells that have ceased to express receptors for mouse erythrocytes and surface IgD.
D000818:Animals; D001402:B-Lymphocytes / Q000145:classification / Q000166:cytology* / Q000276:immunology; D002454:Cell Differentiation; D006801:Humans; D007072:Immunoglobulin D; D007075:Immunoglobulin M; D008213:Lymphocyte Activation; D051379:Mice; D010950:Plasma Cells / Q000166:cytology / Q000276:immunology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D011947:Receptors, Antigen, B-Cell / Q000145:classification; D012397:Rosette Formation
D007072:Immunoglobulin D; D007075:Immunoglobulin M; D011043:Pokeweed Mitogens; D011947:Receptors, Antigen, B-Cell