Decreased actin content of lymphocytes from patients with chronic lymphocytic leukemia.
Actin, a major cytoskeletal protein, was quantitated in normal and chronic lymphocytic leukemia lymphocytes. The actin content of normal human blood lymphocytes was 2.2 +/- 0.4 mg/10(9) cells and represented 6.6% +/- 1.8% of the total cellular protein. A significant decrease (p less than 0.001) was noted in chronic lymphocytic leukemia lymphocytes that contained 1.4 +/- 0.3 mg actin/10(9) cells, constituting 4.3% +/- 1.1% of the total protein. Normal T and B cells did not differ in actin content. Reduced actin levels were found in the T as well as in the B lymphocytes of "B-cell" chronic lymphocytic leukemia. The possible importance of the decreased actin level in the anomalous capping response and motility of chronic lymphocytic leukemia lymphocytes is discussed.
D000199:Actins / Q000097:blood*; D001402:B-Lymphocytes / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D007531:Isoflurophate / Q000494:pharmacology; D007945:Leukemia, Lymphoid / Q000097:blood*; D008214:Lymphocytes / Q000032:analysis*; D013601:T-Lymphocytes / Q000032:analysis
D000199:Actins; D007531:Isoflurophate