The use of TNP-conjugated polyacrylamide beads in a C1q binding inhibition test for circulating immune complexes.
A new assay for the detection of circulating immune complexes is described. It is based on the same principle as the C1q deviation test: the binding of radiolabelled C1q to a solid phase is inhibited by immune complexes. Trinitrophenylated polyacrylamide beads are used as a stable C1q-reactive solid phase in our test. Aggregated IgG in normal serum could be detected by this method to a minimum concentration of about 10 microgram/ml. The test was used to quantitate circulating immune complexes in sera of patients with glomerulonephritis, liver diseases, lymphoma, and myeloid leukemia. The results are compared and correlated with those obtained by the C1q binding assay for the same sera.
D000180:Acrylic Resins / Q000494:pharmacology*; D000208:Acute Disease; D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism*; D001667:Binding, Competitive; D002908:Chronic Disease; D003166:Complement Activating Enzymes / Q000378:metabolism*; D015922:Complement C1q; D005921:Glomerulonephritis / Q000276:immunology; D006509:Hepatitis B / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D008103:Liver Cirrhosis / Q000276:immunology; D008863:Microspheres; D009578:Nitrobenzenes / Q000276:immunology*; D011817:Rabbits; D014301:Trinitrobenzenes / Q000276:immunology*; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000936:Antigen-Antibody Complex; D003166:Complement Activating Enzymes; D015922:Complement C1q; D007074:Immunoglobulin G; D009578:Nitrobenzenes; D014301:Trinitrobenzenes; D000099166:Polyacrylamides