Rapid detection in spinal fluid of beta-lactamase produced by ampicillin-resistant Haemophilus influenzae.
The chromogenic cephalosporin nitrocefin was used to detect the presence of beta-lactamase in cerebrospinal fluid of patients suffering from ampicillin-resistant Haemophilus influenzae meningitis. Five samples of spinal fluid containing ampicillin-resistant isolates were studied, and all had beta-lactamase activity. When samples of spinal fluid containing 33 ampicillin-sensitive isolates and 234 sterile specimens were tested, no beta-lactamase activity was detected.
D000667:Ampicillin / Q000494:pharmacology*; D002555:Cerebrospinal Fluid / Q000382:microbiology; D006193:Haemophilus influenzae / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D008722:Methods; D010403:Penicillin Resistance; D001618:beta-Lactamases / Q000134:cerebrospinal fluid*
D000667:Ampicillin; D001618:beta-Lactamases