Evidence for two populations of B-L (Ia-like) molecules encoded by the chicken MHC.
Two specific alloantisera detecting B-L (Ia-like) antigens on chicken lymphocytes of the B6 and B15 haplotypes were found to cross-react strongly. Anti-B-L6 and anti-B-L15 alloantisera both reacted with B-L molecules on B6 and B15 lymphocytes as demonstrated by immunofluorescence and SDS-PAGE analysis of 125I-labeled B-L antigens isolated by incubation with anti-B-L alloantisera. Absorption studies showed that the anti-B-L alloantisera reacted with at least two kinds of antigenic determinant, one set shared by B-L6 and B-L15 molecules and another set specific for each haplotype. In spite of the absence of genetic evidence for more than one B-L locus in the chicken B complex, it was shown by sequential antibody incubations that these two different B-L antigenic determinants are associated with at least two separate species of B-L molecules, indicating the presence of at least two B-L loci within the MHC of the chicken.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002645:Chickens / Q000235:genetics*; D002874:Chromosome Mapping; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D008285:Major Histocompatibility Complex; D013601:T-Lymphocytes / Q000276:immunology
D000949:Histocompatibility Antigens Class II