title: The rickettsia-like organisms TATLOCK (1943) and HEBA (1959): bacteria phenotypically similar to but genetically distinct from Legionella pneumophila and the WIGA bacterium.
abstract: Two "rickettsia-like organisms," TATLOCK and HEBA, isolated from human blood via guinea pigs and embryonated eggs in 1943 and 1959, respectively, have been cultured on artificial media (charcoal yeast extract agar) for the first time and characterized. TATLOCK and HEBA have identical cultural, biochemical, and antigenic characteristics, as well as identical cellular fatty-acid composition and antimicrobial susceptibilities. These two bacteria have most of the cultural and biochemical characteristics of Legionella pneumophilia, and their gas-liquid chromatography cellular fatty-acid profile is similar to that of WIGA, another bacterium similar to L. pneumophila. Direct fluorescent-antibody reagents prepared for HEBA and TATLOCK gave equal high-titered reciprocal staining and were negative on 220 other bacteria, including L. pneumophila. Deoxyribonucleic acid relatedness studies, however, showed that these bacteria are not genetically related to either L. pneumophila or the WIGA bacterium.
mesh_terms: D000900:Anti-Bacterial Agents; D001431:Bacteriological Techniques; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D007877:Legionnaires' Disease / Q000382:microbiology*; D010641:Phenotype; D012281:Rickettsia / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D004269:DNA, Bacterial; D005227:Fatty Acids