Clinical significance of the anti-complement component of antiglobulin antisera.
The need for anti-complement (anti-C') activity in antiglobulin antisera (AHG) for the detection of clinically significant antibodies was evaluated during a three-year period. While performing routine compatibility testing using standard blood banking procedures, eight patients were found whose antibodies were detectable primarily or only by AHG containing anti-C' activity; monospecific anti-igG AHG gave weak or negative reactions. Seven of the antibodies were anti-jka or jkb. Two of the anti-jka antibodies were responsible for clinically unsuspected delayed hemolytic transfusion reactions. The anti-jkb antibody resulted in a shortened survival of incompatible 51Cr-labelled red blood cells. The incidence of such "complement-only" Kidd antibodies was 23 percent of all Kidd antibodies found. These data suggest that the omission of anti-C' in AHG in routine compatability testing could result in substantial risk of failure to detect clinically significant antibodies.
D000368:Aged; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D001789:Blood Group Antigens / Q000276:immunology*; D001787:Blood Group Incompatibility / Q000175:diagnosis*; D001788:Blood Grouping and Crossmatching / Q000379:methods; D003169:Complement Inactivator Proteins / Q000276:immunology*; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007667:Kidd Blood-Group System / Q000276:immunology*; D008297:Male; D008875:Middle Aged
D000888:Antibodies, Anti-Idiotypic; D001789:Blood Group Antigens; D003169:Complement Inactivator Proteins; D007106:Immune Sera; D007667:Kidd Blood-Group System; C012749:anticomplement