Augmentation of guinea pig T lymphocyte proliferative response to antigens in the presence of purified B cells.
The effect of supplemental B lymphocytes on the antigen-induced in vitro proliferative response of highly purified lymph node T cells was studied in two immune systems of complete Freund's adjuvant (CFA) and testicular antigen (TA) sensitized guinea pigs. When the T lymphocytes obtained from animals 2 weeks after CFA sensitization, though unresponsive to purified protein derivate of tuberculin (PPD) by themselves, were cultured together with mitomycin C-treated autologous B (Bm) lymphocytes (1:0.5 ratio), they responded well to PPD. The enhancing effect of Bm lymphocytes was less than that of mitomycin C-treated macrophages (M phi m) from the same animals. Analogous helper effect of Bm lymphocytes on the T cell proliferation was observed also in TA-sensitized animals. Additionally, it is noteworthy that the PPD-induced proliferation of T lymphocytes at 2 weeks after CFA sensitization was absolutely dependent on M phi and B lymphocytes, whereas T lymphocytes at 10--14 weeks exhibited a low but significant response by themselves, although their response to PPD was markedly augmented with supplemental M phi m or Bm lymphocytes.
D000818:Animals; D000941:Antigens / Q000008:administration & dosage*; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D005620:Freund's Adjuvant / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008264:Macrophages / Q000276:immunology; D008297:Male; D008937:Mitomycins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*; D013737:Testis / Q000276:immunology; D013997:Time Factors; D014373:Tuberculin / Q000276:immunology
D000941:Antigens; D008937:Mitomycins; D014373:Tuberculin; D005620:Freund's Adjuvant