Hydrophobic adsorption chromatography of colony-stimulating activities and erythroid-enhancing activity from the human monocyte-like cell line, GCT.
The human cell line, GCT, secretes hemopoietins into serum-free culture medium. The conditioned medium contains activities that stimulate neutrophil-monocyte, macrophage, eosinophil, and erythroid colony growth in human marrow cultures. We have used hydrophobic adsorption chromatography to separate a neutrophil-monocyte colony-stimulating factor (CSF) from the other colony-stimulating activities. This hydrophobic CSF has no eosinophil-stimulating activity and is virtually devoid of erythroid-stimulating activity.
D000327:Adsorption; D000818:Animals; D002417:Cattle; D002460:Cell Line; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003115:Colony-Stimulating Factors / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D009000:Monocytes / Q000378:metabolism*; D012685:Sepharose / Q000031:analogs & derivatives / Q000494:pharmacology; D014867:Water / Q000378:metabolism*
D003115:Colony-Stimulating Factors; D014867:Water; C020927:phenyl-sepharose; D012685:Sepharose