Temperature-sensitive binding of solid phase C1q to aggregated human immunoglobulin G.
The first component of complement (C1q) coupled to Sepharose by cyanogen bromide was found not to bind aggregated human gamma-globulin or immune complexes at room temperature, whereas at 4 degrees C binding was nearly complete. The temperature sensitivity of solid phase C1q binding was reversible. Elution of aggregated human gamma-globulin bound at 42 degrees C was possible by raising the temperature to 23 degrees C. However, free C1q or C1q adsorbed onto polystyrene balls could bind immune complex-like material at both 23 and 4 degrees C. The conformational restraints of C1q covalently coupled to a solid support may not allow functional activity at elevated temperatures.
D002846:Chromatography, Affinity; D003166:Complement Activating Enzymes / Q000378:metabolism*; D015922:Complement C1q; D003488:Cyanogen Bromide; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D046911:Macromolecular Substances; D012685:Sepharose; D013696:Temperature
D007074:Immunoglobulin G; D046911:Macromolecular Substances; D015922:Complement C1q; D012685:Sepharose; D003166:Complement Activating Enzymes; D003488:Cyanogen Bromide