Induction of cytotoxic lymphocytes by protein A derived from Staphylococcus aureus. I. Protein-A-induced, protein-A-dependent cytotoxicity of T lymphocytes against human lymphoblastoid cell lines.
Protein A (pA) from Staphylococcus aureus is known to be a good mitogen for human T lymphocytes. In this study, the cytotoxic activity of pA-activated lymphocytes was assessed using human lymphoblastoid cell lines (LCL) as target cells. pA induced higher cytotoxic activity against LCL on the 3rd day of incubation than phytohemagglutinin or concanavalin A. However, it was necessary for pA to be present in the assay for these pA-activated lymphocytes to manifest their cytotoxicity. Cytotoxic activity was not detected in a control culture in which pA was added at the assay, nor was it detected in the supernate of pA-activated culture. These data suggest that the cytotoxicity of these lymphocytes was pA-activated, pA-dependent cell-mediated cytotoxicity, pA-activated, pA-dependent cytotoxicity was mediated by T lymphocytes and was induced from Fc-receptor-negative [Fc(-)] T lymphocytes. The effector cells were mainly Fc(-) T lymphocytes. The relationship between pA-activated, pA-dependent cytotoxicity, lectin-dependent cellular cytotoxicity and alloantigen-induced cytotoxic T lymphocytes is also discussed.
D002460:Cell Line; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D006801:Humans; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000494:pharmacology; D011961:Receptors, Fc; D012397:Rosette Formation; D013205:Staphylococcal Protein A / Q000378:metabolism / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D010835:Phytohemagglutinins; D011961:Receptors, Fc; D013205:Staphylococcal Protein A; D003208:Concanavalin A