Preparation of beta-XIIa (Hageman factor fragment) from human plasma.
beta-Factor XIIa (beta-XIIa, Mr approximately 30,000) was isolated from human plasma by a procedure which utilized, as an initial step, the adsorption of Factor XII to celite. Activation of Factor XII and subsequent release of beta-XIIa was brought about by the proteolytic action of co-adsorbed kallikrein. Two successive chromatographic procedures were then used to achieve a final purification of 4,420-fold over plasma and an overall field of 2.3 mg of beta-XIIa per liter.
D000327:Adsorption; D000818:Animals; D001778:Blood Coagulation Disorders / Q000097:blood; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005174:Factor XII / Q000302:isolation & purification*; D015956:Factor XIIa; D006041:Goats; D006801:Humans; D007122:Immunoelectrophoresis; D010446:Peptide Fragments / Q000302:isolation & purification*; D010949:Plasma; D011288:Prekallikrein / Q000032:analysis; D011817:Rabbits
D010446:Peptide Fragments; D005174:Factor XII; D011288:Prekallikrein; D015956:Factor XIIa