Purification of murine T cell growth factor. A lymphocyte mitogen with helper activity.
Mouse T cell growth factor was purified from the serum-free conditioned medium of lectin-stimulated spleen cells. A 3,000-fold purification was achieved with a final yield of 12%. The purified protein, with an apparent Mr of 23,000 by sodium dodecyl sulfate polyacrylamide gel electrophoresis, was active at concentrations of 4 x 10(-11) M, both in the T cell growth factor and T cell replacing factor assays. In addition, purified T cell growth factor alone was mitogenic for spleen cells from both nude and normal mice.
D000818:Animals; D002852:Chromatography, Ion Exchange; D004306:Dose-Response Relationship, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D007376:Interleukin-2 / Q000302:isolation & purification*; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000302:isolation & purification*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008819:Mice, Nude / Q000276:immunology; D012967:Sodium Dodecyl Sulfate
D007376:Interleukin-2; D008222:Lymphokines; D012967:Sodium Dodecyl Sulfate