title: Restricted nucleocytoplasmic relationship in activation of T and B lymphocytes.
abstract: Nuclei of murine T lymphocytes or B lymphocytes were purified and transferred into lethally irradiated whole spleen cells or B or T lymphocytes by means of polyethyleneglycol-mediated cell fusion. Transfer of lymphocyte nuclei could save the irradiated cells from cell death, and such reconstituted cells could respond to mitogens. The present study showed that nuclei of T cells could be activated in the concanavalin A-stimulated T cell cytoplasms but not in the lipopolysaccharide-stimulated B cell cytoplasms. On the other hand, nuclei of B cells were activated in the lipopolysaccharide-stimulated B cells but not in the concanavalin A-stimulated T cell cytoplasms. These data suggested that a specific interaction between cytoplasm and nucleus might exist in the activation of nuclei of each lymphocyte subset.
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000528:radiation effects; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology; D003593:Cytoplasm / Q000276:immunology*; D004247:DNA / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D011975:Receptors, Mitogen / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000528:radiation effects
chemical_list: D008070:Lipopolysaccharides; D011975:Receptors, Mitogen; D003208:Concanavalin A; D004247:DNA