Purification and immunohistochemical localization of rat liver glutathione peroxidase.
Glutathione peroxidase was purified from the rat liver to give a single protein band in polyacrylamide gel electrophoresis. Rabbits were immunized with this purified enzyme, and a highly specific anti-glutathione peroxidase antiserum was obtained. Using this antibody, an immunohistochemical technique (the indirect method of peroxidase-labeled antibody) was applied to study the localization of the enzyme in the liver cells. On immunohistochemical observation, glutathione peroxidase was localized exclusively in the cytoplasm of hepatocytes, and a stronger 'immuno-staining' was exhibited in the peripheries of the hepatic lobules than in the central zone.
D000818:Animals; D003593:Cytoplasm / Q000201:enzymology; D005979:Glutathione Peroxidase / Q000302:isolation & purification*; D007124:Immunoenzyme Techniques; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008970:Molecular Weight; D010544:Peroxidases / Q000302:isolation & purification*; D051381:Rats
D010544:Peroxidases; D005979:Glutathione Peroxidase