title: Effect of methotrexate on phagocytosis and killing of Staphylococcus aureus by human granulocytes.
abstract: The effect of methotrexate on the phagocytosis and killing of Staphylococcus aureus by granulocytes from healthy blood donors was investigated in vitro. A concentration of methotrexate of 2.2 x 10(-4) mol/1 (100 micrograms/ml), which is readily obtained on high dose intravenous administration did not decrease the phagocytosis and intracellular killing of Staph. aureus. In addition, granulocytes isolated from patients after termination of 6 h and 24 h high-dose methotrexate infusions showed normal phagocytic activity.
mesh_terms: D000328:Adult; D006098:Granulocytes / Q000276:immunology*; D006801:Humans; D008727:Methotrexate / Q000494:pharmacology*; D008828:Microbiological Techniques; D010587:Phagocytosis / Q000187:drug effects*; D013211:Staphylococcus aureus
chemical_list: D008727:Methotrexate