Studies on Alternaria allergens. III. Effect of Alternaria tenuis on the humoral response to three T cell-dependent antigens in rats.
The effects of an extract of the saprophytic mold, Alternaria tenuis (AT-CE) on the humoral response to a ragweed allergen extract (DWSR), ovalbumin and sheep red blood cells (SRBC) was investigated in female Wistar rats. Animals pretreated with 100 micrograms or 2 mg AT-CE showed enhancement (p less than 0.05) in the reaginic response (IgE antibody) to DWSR at 25 and 18 days postimmunization. On the other hand, animals posttreated with AT-CE showed substantial reduction in anti-DWSR IgE antibody response. Contrasting results were obtained when ovalbumin was used as an immunizing antigen. There was a remarkable enhancement in the reaginic response to ovalbumin in rats pre- or posttreated with 10 micrograms of AT-CE. Pretreatment with AT-CE did not affect the hemagglutination titers to ovalbumin, while posttreatment with 100 micrograms or 1 mg AT-CE increased the hemagglutination titers of IgM antibody. There was a significant reduction in hemagglutinin, and hemolysin titers to SRBC in animals pretreated with all concentrations of AT-CE; at day 21, suppression was noted in animals pre- or posttreated with all concentrations of AT-CE. On the other hand, greatly increased hemagglutination titers were found in animals posttreated with 100 micrograms or 1 mg AT-CE. Hence, enhancement and suppression can both occur depending on the dose and time of administration of AT-CE together with the nature of the immunizing antigen.
D000528:Alternaria / Q000276:immunology; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000941:Antigens / Q000008:administration & dosage; D005260:Female; D007073:Immunoglobulin E / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008623:Mercaptoethanol / Q000494:pharmacology; D009181:Mycoses / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology
D000941:Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007073:Immunoglobulin E; D008623:Mercaptoethanol; D010047:Ovalbumin