title: Circulating immune complexes in Behçet's syndrome: purification, characterization and cross-reactivity studies.
abstract: The C1q-binding assay was performed on 30 sera from patients with Behçet's syndrome and circulating immune complexes were found in 46%. Circulating immune complexes were isolated and purified from the sera of two patients by immunoadsorption on a column of polymethylmetacrylate beads coated with C1q and then labelled with 125I. In double immunodiffusion these purified immune complexes were found to contain IgG, C1q, C1s and C3. Anti-IgG activity was not detectable in the purified immune complexes while specific cross-reactivity was found in a solid-phase radioimmunoassay with the majority of the sera of behçet's syndrome.
mesh_terms: D000936:Antigen-Antibody Complex / Q000276:immunology / Q000302:isolation & purification*; D001528:Behcet Syndrome / Q000276:immunology*; D003166:Complement Activating Enzymes / Q000276:immunology; D015922:Complement C1q; D003168:Complement Fixation Tests; D003429:Cross Reactions; D006801:Humans; D011863:Radioimmunoassay
chemical_list: D000936:Antigen-Antibody Complex; D015922:Complement C1q; D003166:Complement Activating Enzymes