Chemical damage in gamma-irradiated human erythrocyte membranes.
In air saturated suspensions of erythrocyte ghost membranes gamma-irradiation causes formation of lipid peroxides, measured as malonaldehyde, and a loss of membrane protein sulphydryl groups. Addition of N-(p-amino-benzoyl)-1-glutamate prevented peroxidation up to doses of 2 x 10(3) Gy, due to scavenging of hydroxyl radicals. Another hydroxyl scavenger sodium formate, also prevented peroxidation at low doses, but lost its protective effect at higher doses probably because of secondary reactions of the resulting superoxide radical anion. Two sulphur containing radioprotectants also were able to reduce the extent of lipid peroxidation. The enzymes catalase and superoxide dismutase were added to the irradiated suspensions in order to determine the contribution from hydrogen peroxide and superoxide to peroxidation. The extents of peroxidation are compared with structural modification of the membrane under the same conditions of irradiation.
D010129:4-Aminobenzoic Acid / Q000037:antagonists & inhibitors; D002374:Catalase; D004910:Erythrocyte Membrane / Q000032:analysis / Q000528:radiation effects*; D004912:Erythrocytes / Q000528:radiation effects*; D005561:Formates; D006801:Humans; D007461:Iodoacetates / Q000494:pharmacology; D019807:Iodoacetic Acid; D008054:Lipid Peroxides / Q000097:blood* / Q000528:radiation effects; D011837:Radiation-Protective Agents; D012964:Sodium; D013482:Superoxide Dismutase
D005561:Formates; D007461:Iodoacetates; D008054:Lipid Peroxides; D011837:Radiation-Protective Agents; D012964:Sodium; D002374:Catalase; D013482:Superoxide Dismutase; D010129:4-Aminobenzoic Acid; D019807:Iodoacetic Acid