title: Temperature-sensitive binding of solid phase C1q to aggregated human immunoglobulin G.
abstract: The first component of complement (C1q) coupled to Sepharose by cyanogen bromide was found not to bind aggregated human gamma-globulin or immune complexes at room temperature, whereas at 4 degrees C binding was nearly complete. The temperature sensitivity of solid phase C1q binding was reversible. Elution of aggregated human gamma-globulin bound at 42 degrees C was possible by raising the temperature to 23 degrees C. However, free C1q or C1q adsorbed onto polystyrene balls could bind immune complex-like material at both 23 and 4 degrees C. The conformational restraints of C1q covalently coupled to a solid support may not allow functional activity at elevated temperatures.
mesh_terms: D002846:Chromatography, Affinity; D003166:Complement Activating Enzymes / Q000378:metabolism*; D015922:Complement C1q; D003488:Cyanogen Bromide; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D046911:Macromolecular Substances; D012685:Sepharose; D013696:Temperature
chemical_list: D007074:Immunoglobulin G; D046911:Macromolecular Substances; D015922:Complement C1q; D012685:Sepharose; D003166:Complement Activating Enzymes; D003488:Cyanogen Bromide