Cell-mediated immunity to herpes simplex virus: specificity of cytotoxic T cells.
This communication deals with the question of which of the viral antigens constitutes the targets for cytotoxic T lymphocytes (CTL) generated against herpes simplex virus type 1 (HSV-1). The approach used was, first, to compare cytotoxicity of CTL against target cells infected with virus in the presence of tunicamycin and 2-deoxy-D-glucose, which are known to inhibit glycoprotein synthesis, and second, to compare cytotoxicity of CTL against target cells infected with wild-type HSV-1 with that against target cells infected with a temperature-sensitive mutant of HSV-1 which, at the nonpermissive temperature, exhibits diminished glycoprotein synthesis. The results show that glycoprotein expression is required for the demonstration of cytotoxic activity of CTL. The level of cytotoxicity against the temperature-sensitive HSV-1 target at the nonpermissive temperature was reduced and correlated with the level of expression of the major envelope glycoprotein region (VP123; molecular weight = 123,000) at the target cell surface as measured serologically by antibody binding studies. The results were interpreted to indicate that HSV-1-induced glycoproteins are the target antigens for anti-HSV CTL and that the principal viral antigens recognized by the CTL may be glycoproteins of the VP123 region.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D003602:Cytotoxicity, Immunologic; D003847:Deoxyglucose / Q000494:pharmacology; D006023:Glycoproteins / Q000276:immunology; D006183:H-2 Antigens; D006561:Herpes Simplex / Q000276:immunology*; D007111:Immunity, Cellular; D007739:L Cells; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*; D014415:Tunicamycin / Q000494:pharmacology; D014764:Viral Proteins / Q000276:immunology
D000956:Antigens, Viral; D006023:Glycoproteins; D006183:H-2 Antigens; D014764:Viral Proteins; D014415:Tunicamycin; D003847:Deoxyglucose