title: Characterization of in vivo suppression of syngenic tumor by allogenic effector cells.
abstract: The purpose of this study was to characterize the effects of allogenic splenocytes transferred with tumor cells, subcutaneously, to a host syngenic for tumor cells. Cytotoxic effector cells usually resulted in tumors less than half the size of controls at E:T of 10:1. Primary immune splenocytes were more effective than hyperimmune splenocytes in delaying tumor growth. Actively cytotoxic splenocytes by in vitro 51Cr release assay were required for delayed tumor growth; memory cell populations were not effective. Delayed tumor growth correlated with in vitro cytotoxicity of primary immune splenocytes; however, hyperimmune splenocytes, even though they possessed greater in vitro cytotoxic responses, showed lesser tumor suppression in vivo. T cells were necessary for tumor suppression, as treatment of B6AF1 effector cells with anti-Thy 1.2 serum abrogated suppression; T cell enrichment by nylon-wool treatment of effector cells increased tumor suppression. Delay in tumor growth was an in vivo phenomenon, for anti-Thy 1.2 serum in AKR hosts abrogated the effect of Thy 1.2 effector cells.
mesh_terms: D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens; D007167:Immunotherapy; D051379:Mice; D008806:Mice, Inbred AKR; D008811:Mice, Inbred DBA; D008954:Models, Biological; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000628:therapy; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous
chemical_list: D000961:Antilymphocyte Serum; D006183:H-2 Antigens