Effect granulocytic factors on in vitro and in vivo activity of cytotoxic cells of guinea pigs.
The effect of factors obtained from ascites granulocytes of guinea pigs was studied on the cytotoxic activity of spleen cells of guinea pigs. In the investigations carried out as yet it has been demonstrated that the lysosomes of neutrophils contain factors stimulating blastic transformation of lymphocytes. Exogenous mitogens such as PHA, Con A, stimulate the cytotoxic activity and in view of this the cytotoxic effect was investigated using radioactive 51Cr for labelling target cells (cock erythrocytes). It was found that the studied factors inhibited the spontaneous cytotoxic activity of guinea pig spleen cells (natural killer (NK) cell activity) but had no effect on the cytotoxic activity of PHA-stimulated spleen cells. In in vivo experiments it has been demonstrated that they had an inhibitory effect on the development and the degree of expression of specifically sensitized cytotoxic cells. The mechanism of this effect has not yet been elucidated completely.
D000818:Animals; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006098:Granulocytes / Q000502:physiology*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008247:Lysosomes / Q000187:drug effects; D013154:Spleen / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology