Lymphocyte activation by purified carrageenan.
The induction of polyclonal antibody production and mitogenic response by purified carrageenan was studied in vitro by using spleen cells. It was shown that kappa-CGN, lambda-CGN, and iota-CGN induced significant responses in mice, hamsters, and rats. The less purified CGN also exhibited the polyclonal antibody production in mice. In addition, purified CGN exhibited mitogenic action on B cells and to a lesser extent, on thymocytes. Polyclonal antibody responses induced by purified CGN were not affected by removal of T cells, adherent cells, or iron powder phagocytic cells. These results suggest that CGN may activate B cells resulting in polyclonal antibody responses.
D000818:Animals; D000917:Antibody Formation; D002351:Carrageenan / Q000302:isolation & purification / Q000494:pharmacology*; D006224:Cricetinae; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors; D014301:Trinitrobenzenes / Q000276:immunology
D014301:Trinitrobenzenes; D002351:Carrageenan