An assay for virus-specific help for B cells.
Spleen cells from mice immunized with vaccinia or influenza viruses were mixed with spleen cells from mice immunized by dinitrophenyl-keyhole limpet hemocyanin (DNP-KLH) and transferred into irradiated syngeneic recipient mice which were subsequently restimulated with trinitrophyenylates (TNP) or unmodified viruses. One week later, spleens were removed and assayed for indirect anti-DNP plaque-forming cells (PFC). When spleen cells from both influenza and vaccinia primed mice were restimulated with the haptenated form of the virus used for the initial immunization there was an enhanced PFC response compared to that seen with spleen cells from unprimed mice.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D004136:Dinitrobenzenes / Q000276:immunology; D006433:Hemocyanins / Q000276:immunology; D009980:Influenza A virus / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D013154:Spleen / Q000276:immunology; D014616:Vaccinia virus / Q000276:immunology*
D004136:Dinitrobenzenes; D006433:Hemocyanins