Serologic comparison of a monoclonal anti-C3d with commercial antiglobulin reagents.
The serologic reactivity of a monoclonal anti-C3d and commercial "monospecific" anti-C3 and polyspecific antiglobulin reagents was compared. The testing consisted of a direct antiglobulin test performed on donor and patient cells and on in vivo and in vitro complement-coated cells; an indirect antiglobulin test was performed with low-ionic-strength solution crossmatches. The monoclonal anti-C3d demonstrated reactivity very similar to that of the commercial heterogeneous rabbit anti-C3d; it gave relatively few weak "false positive" reactions with normal donor and patient red blood cells, and comparably strong reactions with in vitro and in vivo C3d-coated red blood cells. The results suggest a promising future for monoclonal antiglobulin reagents.
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D003181:Complement C4 / Q000276:immunology; D003298:Coombs Test; D003429:Cross Reactions; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D009994:Osmolar Concentration
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D003181:Complement C4