The effect of T cell growth factor on the cell cycle of primate T cells.
The effect of T cell growth factor (TCGF) on the cell cycle of four T cell lines that differed in their response to TCGF was studied. Activated normal marmoset T cells (OH-1) were totally dependent on the addition of TCGF for long-term proliferation. In the absence of TCGF, the cells were incapable of traversing the cell cycle and became arrested in G1. The addition of TCGF to arrested OH-1 cells stimulated them to enter the S phase after a lag phase of 24 to 33 hr. The TCGF-stimulated cells reached a maximum of cells in the S phase by 12 hr after the initiation of DNA synthesis. TCGF was required for a minimum of 18 hr before cells would enter the S phase. In the absence of TCGF, activated owl monkey (8I) and human (RG) lymphocytes displayed a TCGF-sensitive block; addition of TCGF stimulated these cells to enter the S phase after 12 and 16 hr, respectively. In contrast, an owl monkey tumor-derived T cell line (OMT-1), not dependent on exogenous TCGF for proliferation, was able to progress slowly through the cell cycle without a TCGF-sensitive block. These cells responded to TCGF by showing an initial increase in cells that entered the S phase after a lag of 6 hr and a continuing movement of additional cells into S over the course of the next several hours.
D000818:Animals; D001029:Aotus trivirgatus; D002453:Cell Cycle; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D007399:Interphase; D012452:Saguinus; D013601:T-Lymphocytes / Q000166:cytology*; D013997:Time Factors
D003470:Culture Media; D007376:Interleukin-2; D004247:DNA