Suppression of hen egg-white lysozyme (HEL)- specific delayed -type hypersensitivity responses in mice by suppressor T cells after neonatal administration of anti-idiotypic antibodies.
Anti-idiotypic rabbit antiserum (anti-Id) directed to the idiotypes of anti-hen egg-white lysozyme (HEL) antibody froma single C3H mouse (No. 2) was shown to be capable of recognizing only a fraction of the anti-HEL antibody populations produced by other C3H mice. Experiments were performed to examine the effect of this particular anti-Id on the delayed-type hypersensitivity (DTH) response specific for the same protein antigen. A group of 60-day-old C3H mice which had been administered anti-Id within 24 hr after birth were tested for HEL-DTH response. The results indicated that the DTH response was completely suppressed by the anit-Id treatment. The inhibition of DTH reactivity is due to active suppression and involves the generation of suppressor T cells. Thus, the suppression induced with a single injection of anti-Id was transferable with both spleen cells and thymocytes from mice, that received anti-Id. These suppressor cells are T cells since their ability to suppress DTH is completely abrogated by treatment in vitro with anti-Thy 1.2 serum and complement.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D003165:Complement System Proteins / Q000494:pharmacology; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007165:Immunosuppression Therapy; D051379:Mice; D008809:Mice, Inbred C3H / Q000276:immunology; D009113:Muramidase / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000961:Antilymphocyte Serum; D007130:Immunoglobulin Idiotypes; D003165:Complement System Proteins; D009113:Muramidase