Variability of short-term cultures of HeLa S-3 cells: changes in DNA distributions and rates of DNA synthesis.
DNA distributions of HeLa S-3 cells in spinner culture exhibit significant time-dependent changes. The major differences appear to occur in the S-phase region. Significant changes in the rates of DNA synthesis in several S-phase subcompartments correlated well with the changes in the DNA distributions. It is proposed that fluctuations in these rates of DNA synthesis are a reflection of the inherent instability of these abnormal, heteroploid cells.
D002453:Cell Cycle; D003201:Computers; D003470:Culture Media; D003584:Cytological Techniques; D004247:DNA / Q000032:analysis / Q000096:biosynthesis*; D006367:HeLa Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007399:Interphase; D007700:Kinetics
D003470:Culture Media; D004247:DNA