Immunoassay of antigens and haptens by inhibition of passive immune hemolysis.
Improved methods are presented for the detection of antigens and haptens by the use of the passive hemolysis inhibition test. The test is capable of detecting nanogram quantities of proteins (e.g., ferritin, IgE) and haptens (e.g., folinic acid, methotrexate). The method is also useful for studying quantitative and qualitative aspects of antibody-antigen interaction.
D000818:Animals; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex / Q000032:analysis; D000941:Antigens / Q000032:analysis*; D003168:Complement Fixation Tests / Q000379:methods*; D003429:Cross Reactions; D004306:Dose-Response Relationship, Immunologic; D004912:Erythrocytes / Q000276:immunology; D006241:Haptens / Q000032:analysis*; D006461:Hemolysis; D007114:Immunization; D007700:Kinetics; D002955:Leucovorin / Q000097:blood / Q000276:immunology; D008727:Methotrexate / Q000276:immunology; D011817:Rabbits
D000936:Antigen-Antibody Complex; D000941:Antigens; D006241:Haptens; D002955:Leucovorin; D008727:Methotrexate