The measurement of cytotoxicity by the reduction of hybridoma plaque-forming cells.
Hybridoma cells secreting antibody against heterologous erythrocytes have been used as targets to measure cytotoxic T cell activity. The ability of the target cells to form hemolytic plaques has been used as an index of target viability. It has been possible to devise a sensitive plaque reduction method for measuring cytotoxicity which lends itself to screening small numbers of cytotoxic cells.
D000818:Animals; D000917:Antibody Formation; D002999:Clone Cells / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006822:Hybrid Cells / Q000276:immunology*; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*