Mitogenic and antigenic properties of group A streptococcal M protein preparations.
The separation of a mitogenic substance in M protein preparations of Streptococcus pyogenes, type 1 and type 12, is described. The isolation was achieved by gel chromatography on Biogel A 0.5 m, and by immunochromatography on immobilized type specific antibodies. In the delayed cutaneous hypersensitivity test the immunochromatography purified M 1 protein caused erythema but no unspecific mitogenicity could be found by lymphocyte transformation test. In neutralization experiments the mitogenic activity of M protein preparations was specifically inhibited by anti-erythrogenic toxin antisera.
D000328:Adult; D000818:Animals; D000918:Antibody Specificity; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000276:immunology* / Q000302:isolation & purification; D002352:Carrier Proteins; D005591:Chemical Fractionation; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D006801:Humans; D006851:Hydrochloric Acid / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology; D008875:Middle Aged; D008934:Mitogens / Q000494:pharmacology*; D009500:Neutralization Tests; D011817:Rabbits; D013297:Streptococcus pyogenes / Q000276:immunology*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D008934:Mitogens; C014689:streptococcal M protein; D006851:Hydrochloric Acid