Cytochemical study in human lymphocyte subpopulations as defined by Fc receptors.
Three subpopulations of human T lymphocytes (TG, TM, and Tnon-G/non-M cells) were evaluated by means of six different cytochemical reactions. Elevated staining activity was demonstrated in all the populations when stained for acid phosphatase (AcP), beta-glucuronidase (BG) and N-acetyl beta-glucosaminidase (NABG). While the majority of TM cells displayed an intense acid alpha-naphthyl acetate esterase (ANAE) positivity, only a smaller proportion of the Tnon-G/non-M exhibited a positive reaction and a much lower activity was found in the TG group. TM cells were also alpha-naphthyl acetate esterase (NAE)-and alpha-naphthyl butyrate esterase (NBE)-positive, showing a dot-like pattern. These reactions were present in a lower proportion in the Tnon-G/non-M population and nearly completely absent in the TG cells. The present study identifies different cytochemical phenotypes for the different T-lymphocyte subsets and the analysis of these phenotypes may indicate that the TM is the most cytochemically differentiated subpopulation.
D000118:Acetylglucosaminidase; D000135:Acid Phosphatase; D002265:Carboxylic Ester Hydrolases; D003594:Cytoplasmic Granules / Q000032:analysis; D005966:Glucuronidase; D006651:Histocytochemistry; D006801:Humans; D009283:Naphthol AS D Esterase; D011961:Receptors, Fc / Q000032:analysis*; D013601:T-Lymphocytes / Q000032:analysis* / Q000145:classification
D011961:Receptors, Fc; D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases; C021855:naphthylbutyrate esterase; D000135:Acid Phosphatase; D005966:Glucuronidase; D000118:Acetylglucosaminidase