A comparison of the sensitivity of immunofluorescence and Giemsa for staining Chlamydia trachomatis inclusions in cycloheximide-treated McCoy cells.
In a clinical study of 190 men with non-gonococcal urethritis, Chlamydia trachomatis inclusions were sought in cycloheximide-treated McCoy cells by an indirect immunofluorescent antibody technique. The method was consistently reliable over a period of two years, and the results were obtained within 24 hours of a patient's attendance. The results correlated with those obtained by Giemsa staining in 91.6% of patients, and the new method was at least as sensitive as the established Giemsa-staining method.
D001399:Azure Stains; D002460:Cell Line; D002690:Chlamydia Infections / Q000175:diagnosis; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D003513:Cycloheximide / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D010640:Phenothiazines; D014526:Urethritis / Q000175:diagnosis
D001399:Azure Stains; D010640:Phenothiazines; D003513:Cycloheximide