Evaluation of a tissue transport medium for immunological characterization of benign and malignant lymphoid tissues.
A tissue transport medium was evaluated for its usefulness in immunologic characterization of lymphocytes in tissue sections. A variety of lymphoid tissues were studied, including normal tissue, hyperplastic tissue and malignant lymphoma. Approximately equal parts of biopsy samples were taken and one part was immediately snap-frozen while the other was held in transport medium for up to one week before processing for frozen section. Sections were stained with fluorescein-conjugated anti-immunoglobulin antisera and with a variety of murine monoclonal antibodies to T-cell antigens or Ia antigen followed by staining with fluorescein-conjugated goat-antimouse gamma globulin. In all cases studied, there was complete agreement in the staining patterns of tissues which were either immediately snap-frozen or held in transport medium.
D000911:Antibodies, Monoclonal / Q000032:analysis; D001402:B-Lymphocytes / Q000032:analysis; D003470:Culture Media; D005069:Evaluation Studies as Topic; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D008221:Lymphoid Tissue / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology*; D013048:Specimen Handling / Q000379:methods*; D013601:T-Lymphocytes / Q000032:analysis
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007136:Immunoglobulins