Nuclear magnetic resonance spectrometric assay of beta-lactamase.
Beta-Lactam antibiotics and the crude enzyme were mixed in deuterium oxide and placed in a nuclear magnetic resonance tube. The change of the nuclear magnetic resonance spectrum during the enzymatic reaction was then analyzed to determine beta-lactamase activity. By using beta-lactam antibiotics such as penicillins, cephalosporins, and cephamycins as substrates, a comparison of the beta-lactamase activities was made between the nuclear magnetic resonance spectrometric assay and the iodometric assay. There was a close correlation between these two methods.
D004926:Escherichia coli / Q000187:drug effects; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D010406:Penicillins / Q000378:metabolism / Q000494:pharmacology; D013379:Substrate Specificity; D013997:Time Factors; D001618:beta-Lactamases / Q000032:analysis*
D010406:Penicillins; D001618:beta-Lactamases