Cytolytically active murine T-cell hybrids.
Following the demonstration that hybrids between normal B-lymphocytes and myeloma cell lines continue to secrete antibodies with the same specificity as those produced by the parental B-cells, many groups have tried to use this approach to obtain cell lines expressing T-lymphocyte functions by crossing thymoma lines not expressing any measurable activity with various types of T-cell populations. Although there have been reports that hybrids could be isolated which secrete T-cell products with immunological activity, efforts to produce functionally active hybrids from cytolytic T-cells have all been unsuccessful (refs 6, 7, and M. N. and H. D. Engers, unpublished). We have fused an established, T-cell growth factor (TCGF)-dependent murine cytolytic T-lymphocyte (CTL) line with a mouse thymoma line and have obtained hybrids with cytolytic activity when we selected the hybrids in TCGF-containing medium, while hybrids isolated in the absence of growth factor showed no detectable cytolytic potential.
D000818:Animals; D002455:Cell Division; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D006133:Growth Substances / Q000494:pharmacology; D006183:H-2 Antigens; D006822:Hybrid Cells / Q000276:immunology; D051379:Mice; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D006133:Growth Substances; D006183:H-2 Antigens; D003208:Concanavalin A