Simultaneous measurement of 5-hydroxytryptophan and L-dihydroxyphenylalanine by high-performance liquid chromatography with electrochemical detection. Measurement of serotonin and catecholamine turnover in discrete brain regions.
A sensitive method, using reversed-phase high-performance liquid chromatography with electrochemical detection, which allows the measurement of both 5-hydroxytryptophan and L-dihydroxyphenylalanine is described. This method, used in conjunction with the administration in vivo of an L-aromatic amino acid decarboxylase inhibitor, allows the simultaneous measurement of serotonin and catecholamine synthesis rates in small discrete brain regions.
D006916:5-Hydroxytryptophan / Q000032:analysis*; D000818:Animals; D001592:Benzyl Alcohols / Q000494:pharmacology; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002395:Catecholamines / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006834:Hydrazines / Q000494:pharmacology; D007700:Kinetics; D007980:Levodopa / Q000032:analysis*; D008297:Male; D008832:Microchemistry; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*
D001592:Benzyl Alcohols; D002395:Catecholamines; D006834:Hydrazines; D012701:Serotonin; D007980:Levodopa; C004297:3-hydroxybenzylhydrazine; D006916:5-Hydroxytryptophan