Ultrastructural studies on the lymphocyte subpopulations of disaggregated mouse spleens.
The lymphocyte subpopulations of disaggregated spleens of adult C57Bl/6J mice were examined by transmission and scanning electron microscopy as well as by rosette formation. The results showed that most lymphocyte subpopulations are constituted of cells with relatively smooth surfaces. Rosette formation showed that the proportion of E rosettes, compatible with the presence of T cells, was of 25%, whereas that of erythrocyte antibody complement (EAC) rosettes, indicating B cells, was 9%.
D000818:Animals; D001402:B-Lymphocytes / Q000648:ultrastructure; D002462:Cell Membrane / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D008214:Lymphocytes / Q000648:ultrastructure*; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009000:Monocytes / Q000648:ultrastructure; D009940:Organoids / Q000648:ultrastructure; D012397:Rosette Formation; D013154:Spleen / Q000166:cytology*; D013601:T-Lymphocytes / Q000648:ultrastructure