Development and characterization of allospecific long-term human cytolytic T-cell lines.
Two long-term human cytolytic T lymphocyte (CTL) lines (VE and JR), whose cytolytic activity was dependent upon both irradiated JY cells (the stimulating alloantigen) and T-cell growth factor, were established. These lines were monitored in culture for 6-8 months. Both lines were specific for HLA-A or B antigens or both and the JR line was allospecific for HLA-B7. These CTL lines killed specific target cells at an effector-to-target ratio of 0.4 (VE) or 0.08 (JR). All of the cells, which grow in suspension, rosetted with sheep erythrocytes and reacted with an antiserum specific for human T cells. The CTL line VE was used to raise rabbit antisera that immunoprecipitated two specific polypeptides (78,000 and 33,000 daltons) from labeled membranes of these CTL lines.
D000818:Animals; D001769:Blood; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D008213:Lymphocyte Activation; D051379:Mice; D011233:Precipitin Tests; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D006133:Growth Substances