Loss of receptor activity for mouse erythrocytes precedes tumour promoter-induced maturation of chronic lymphocytic leukaemia cells.
The tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) induced differentiation in lymphocytes from patients with chronic lymphocytic leukaemia (CLL). The differentiation was detected by the appearance of cytoplasmic immunoglobulin (CIg) and a plasmacytoid morphology, and was accompanied by the loss of the ability of the CLL cells to rosette with mouse erythrocytes (M). Loss of M rosetting occurred rapidly (within 10 min), was not prevented by cycloheximide or actinomycin D and was elicited by low concentrations of TPA (ED50 8 X 10(-10) M). Inhibition of rosette formation was induced by other phorbol diester promoters, but not non-promoting derivatives. Loss of the capacity to form M rosettes occurs before events which are evidently related to genome activation.
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004224:Diterpenes; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007945:Leukemia, Lymphoid / Q000276:immunology*; D051379:Mice; D010703:Phorbol Esters / Q000494:pharmacology*; D010704:Phorbols / Q000494:pharmacology*; D011947:Receptors, Antigen, B-Cell / Q000276:immunology; D011971:Receptors, Immunologic / Q000187:drug effects*; D012397:Rosette Formation; D013729:Terpenes; D013997:Time Factors
D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D010703:Phorbol Esters; D010704:Phorbols; D011947:Receptors, Antigen, B-Cell; D011971:Receptors, Immunologic; D013729:Terpenes; C011309:mezerein