title: Influenza virus-specific cytotoxic T cells are restricted by multiple HLA-A3-related self antigens: evidence for recognition of distinct self structures in conjunction with different foreign antigens.
abstract: In the experiments described in this report we examined the similarities and differences in target cell recognition by influenza-immune CTL that share HLA-A3 but no other defined HLA-A, -B, or -C specificity with each of a panel of virus-infected target cells. Virus-immune CTL populations obtained from selected HLA-A3-positive donors can distinguish between the virus-infected target cells of unrelated donors, as assessed by direct cytotoxicity and cold target inhibition. The CTL restriction antigens that are associated with HLA-A3 in the population are encoded by gene(s) that segregate with HLA-A3 in an HLA-A/C recombinant family. These results suggest that HLA-A3-positive cells from unrelated individuals may differ with respect to 1 or more individual CTL restriction antigens associated with their HLA-A3 molecules, and that each of these distinct restriction antigens is recognized by a different subpopulation of virus-immune CTL. Analysis of the self specificity of T cells from individual donors sensitized against 2 noncross-reacting viruses, type A and type B influenza, indicated that CTL can recognize different foreign antigens in conjunction with different HLA-A3-related self antigens. These results are consistent with a model that proposes that each HLA-A and -B molecule possesses multiple CTL restriction antigens, each of which may function as self recognition structures for CTL that respond to different foreign antigens.
mesh_terms: D001667:Binding, Competitive; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D005192:Family Health; D006680:HLA Antigens; D006801:Humans; D009980:Influenza A virus / Q000276:immunology; D007251:Influenza, Human / Q000276:immunology*; D014176:Protein Biosynthesis; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D006680:HLA Antigens