Demonstration of a third structurally distinct human Ia beta chain by two-dimensional gel electrophoresis.
Previous studies have indicated that HLA-DR homozygous cell lines express two Ia alpha and Ia beta chains that combine to form at least two Ia molecules. This report demonstrates by two-dimensional gel electrophoresis the existence of a third structurally distinct human Ia beta chain on DR2 and DR5 cell lines. This suggests that at least five separate genes control the expression of Ia molecules on HLA-DR homozygous cell lines.
D001402:B-Lymphocytes; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006680:HLA Antigens / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000302:isolation & purification*; D006720:Homozygote; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D046911:Macromolecular Substances; D010455:Peptides / Q000032:analysis*; D009626:Terminology as Topic
D006680:HLA Antigens; D000949:Histocompatibility Antigens Class II; D046911:Macromolecular Substances; D010455:Peptides