In vitro DNA synthesis in lymphocytes from DNCB contact-sensitive guinea pigs.
In vitro stimulation of lymphocytes from DNCB contact-sensitive guinea pigs is achieved by several DNP conjugates. Optimal results are obtained by DNP-modified macrophages. The degree of in vitro lymphocyte activity, demonstrated by DNA synthesis, depends on the cell origin, the interval between sensitization and harvest of lymphocytes and the immunological status of the animals.
D000818:Animals; D001557:Benzenesulfonates; D003520:Cyclophosphamide / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D004136:Dinitrobenzenes; D004137:Dinitrochlorobenzene / Q000276:immunology*; D004139:Dinitrofluorobenzene / Q000276:immunology; D005260:Female; D005620:Freund's Adjuvant; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000276:immunology* / Q000378:metabolism; D007108:Immune Tolerance; D066298:In Vitro Techniques; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D008297:Male; D009578:Nitrobenzenes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D001557:Benzenesulfonates; D004136:Dinitrobenzenes; D004137:Dinitrochlorobenzene; D009578:Nitrobenzenes; C001073:2,4-dinitrobenzenesulfonic acid; D003520:Cyclophosphamide; D004247:DNA; D005620:Freund's Adjuvant; D004139:Dinitrofluorobenzene