The rapid purification of 3-hydroxybutyrate dehydrogenase and malate dehydrogenase on triazine dye affinity matrices.
3-Hydroxybutyrate dehydrogenase (EC 1.1.1.30) and malate dehydrogenase (EC 1.1.1.37) were purified to homogeneity on a large scale involving only two sequential affinity-chromatography steps on two triazine dye-Sepharose matrices. Recoveries of both enzymes were in excess of 60%. Malate dehydrogenase could also be purified by a combination of triazine dye affinity chromatography and gel filtration on Ultrogel AcA-44, but this offered no significant advantage over the purely affinity procedure.
D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel; D004396:Coloring Agents; D006884:Hydroxybutyrate Dehydrogenase / Q000302:isolation & purification*; D008291:Malate Dehydrogenase / Q000302:isolation & purification*; D012242:Rhodobacter sphaeroides / Q000201:enzymology; D012685:Sepharose; D014227:Triazines
D004396:Coloring Agents; D014227:Triazines; D012685:Sepharose; D006884:Hydroxybutyrate Dehydrogenase; D008291:Malate Dehydrogenase