Synthesis of P1 ban protein in minicells infected by P1 mutants.
Phage P1 encodes a dnaB  analog (ban) protein. Synthesis of ban protein has been studied in minicells infected by P1 mutants and has been identified as a polypeptide of 56,000 molecular weight by immunoprecipitation using antibody directed against E. coli dnaB protein. The amount of ban protein synthesized by P1 mutants increases in the order: P1 wild type, P1bac, P1crr, and P1bac crr. The relative amount of ban protein identified in P1bac- and P1bac crr-infected minicells is approximately the same as that previously found in dnaB.ban heteromultimers isolated from the corresponding P1 lysogens.
D003090:Coliphages / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D008242:Lysogeny; D009154:Mutation; D014764:Viral Proteins / Q000096:biosynthesis*
D014764:Viral Proteins