Selective killing of normal or neoplastic B cells by antibodies coupled to the A chain of ricin.
Highly specific antibodies (affinity-purified or hybridoma) directed against cell surface immunoglobulins on normal or neoplastic murine B lymphocytes were covalently coupled to the A chain of the plant toxin ricin. Such conjugates containing antibodies specific for IgM, for either of the two allotypes of IgD, or for the idiotype of the B cell tumor BCL1 rapidly bound in vitro to cells expressing the corresponding surface antigen and inhibited protein synthesis in such cells. The results demonstrate that A chain-coupled anti-idiotype antibody may be useful as a tumor-specific cytotoxic agent.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000918:Antibody Specificity; D000961:Antilymphocyte Serum; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002999:Clone Cells / Q000276:immunology; D006822:Hybrid Cells / Q000276:immunology; D007126:Immunoglobulin Allotypes / Q000276:immunology; D007072:Immunoglobulin D / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology; D010446:Peptide Fragments / Q000494:pharmacology; D014176:Protein Biosynthesis; D012276:Ricin / Q000008:administration & dosage* / Q000494:pharmacology
D000888:Antibodies, Anti-Idiotypic; D000961:Antilymphocyte Serum; D007126:Immunoglobulin Allotypes; D007072:Immunoglobulin D; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M; D010446:Peptide Fragments; D012276:Ricin