The repair of potentially lethal damage in x-irradiated cultures of normal and ataxia telangiectasia human fibroblasts.
The repair of potentially lethal damage (PLD) after X-rays was studied in plateau phase cultures of nine normal and five ataxia telangiectasia (AT) strains of human fibroblasts. In the normal strains PLD repair was complete after 6 hours of post-irradiation incubation. There were differences in the form of the survival curves of normal strains after maximum PLD repair but the extent of post-irradiation recovery was similar in all strains. In contrast all AT strains were almost completely deficient in PLD repair even when post-irradiation incubation was extended to 18 or 24 hours. The relevance of the PLD repair-deficiency in cultured AT strains to in vivo radiotherapeutic observations in AT patients is briefly discussed.
D000328:Adult; D001260:Ataxia Telangiectasia / Q000235:genetics* / Q000532:radiotherapy; D002460:Cell Line; D002648:Child; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005347:Fibroblasts / Q000528:radiation effects*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D013997:Time Factors; D014965:X-Rays