Isoelectric focusing pattern of acid phosphatase and acid esterase in human blood cells, including thymocytes, T lymphocytes, and B lymphocytes.
Lysosomal acid phosphatase and acid esterase from highly purified, viable human thymocytes, T lymphocytes, B lymphocytes, granulocytes, monocytes, platelets, and erythrocytes were subjected to direct measurement of activity and to isoelectric focusing in polyacrylamide thin-layer slabs. The typical patterns for each cell line were found to be distinctly different from the results recently presented in this journal. The study described here opens a new possibility for the biochemical identification of cells and their functional derivatives and neoplastic variants.
D000135:Acid Phosphatase / Q000235:genetics*; D001402:B-Lymphocytes / Q000201:enzymology; D001773:Blood Cells / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology; D004950:Esterases / Q000235:genetics*; D006098:Granulocytes / Q000201:enzymology; D006801:Humans; D007525:Isoelectric Focusing; D009000:Monocytes / Q000201:enzymology; D011110:Polymorphism, Genetic; D013601:T-Lymphocytes / Q000201:enzymology
D004950:Esterases; D000135:Acid Phosphatase