Improved reaction buffers for solid-phase enzyme immunoassay without interference by serum factors.
A gelatin moderately digested by a protease, was as effective as original gelatin to remove the interference by serum factors with a sandwich immunoassay for insulin. Digestion of gelatin resulted in decrease in the viscosity, and the buffer containing the digested gelatin did not show gelation at 4 degrees C. Therefore the buffer involving the digested gelatin might be widely useful for the immunoassay without interference by serum factors.
D002021:Buffers; D003080:Cold Temperature; D005780:Gelatin; D006801:Humans; D007124:Immunoenzyme Techniques; D007328:Insulin / Q000097:blood*; D010447:Peptide Hydrolases / Q000494:pharmacology; D014783:Viscosity
D002021:Buffers; D007328:Insulin; D005780:Gelatin; D010447:Peptide Hydrolases