Distribution and quantitation of HLA-ABC and DR (Ia) antigens on human kidney and other tissues.
A quantitative estimation of the amounts of human Ia (HLA-DR) and HLA-ABC antigen on a variety of human tissues was performed. Monoclonal antibodies to species-common determinants of HLA-DR and HLA-ABC antigens were absorbed quantitatively with tissue homogenates and cell suspensions, and reassayed for residual activity in a radioimmunobinding assay. Kidney was found to carry 90% as much HLA-DR and 14% as much HLA-ABC antigen as spleen, while liver contained 19 and 9% as much, respectively. Small amounts of both antigens were found on heart; brain carried very little HLA-ABC and virtually no DR. Neither HLA-ABC nor DR was found on erythrocytes or reticulocytes. Of interest was our finding that a small subpopulation of thymocytes (10%) was HLA-DR positive. Platelets contained approximately 5% of the amount of HLA-ABC as spleen and undetectable quantities of HLA-DR, as expected. Chronic lymphatic leukemia (CLL) cells were found to carry 10% as much HLA-ABC and 33% as much DR antigen as spleen, while the values for bone marrow were 15 and 2%, respectively.
D005455:Fluorescent Antibody Technique; D006680:HLA Antigens / Q000032:analysis*; D006801:Humans; D007163:Immunosorbent Techniques; D007519:Isoantigens / Q000032:analysis*; D007668:Kidney / Q000276:immunology*; D008099:Liver / Q000276:immunology; D009206:Myocardium / Q000276:immunology; D011863:Radioimmunoassay; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014018:Tissue Distribution
D006680:HLA Antigens; D007519:Isoantigens