A technique for the flow cytometric analysis of lymphocytes bearing histamine receptors.
Histamine receptors have been demonstrated on lymphocyte membranes by a variety of techniques. We now report a method that allows for the flow cytometric analysis of histamine receptors on human peripheral T cells. Histamine is conjugated to fluoresceinated human albumin by the coupling agent ECDI. This conjugated histamine compound (FHA-his) binds to approximately 45% of T cells. Fluoresceinated human albumin alone (FHA), not conjugated to histamine, does not bind to T cells. In addition, unconjugated histamine can inhibit completely the binding seen with FHA-his. We conclude that this technique demonstrates specific FHA-his binding to histamine receptors on T cells and can be used to determine the number of cells bearing such receptors. In addition, the reagent could be used with a cell sorter to isolate distinct histamine-receptor-bearing (HR+) cells for further immunologic study.
D001772:Blood Cell Count; D005452:Fluoresceins / Q000378:metabolism; D006632:Histamine / Q000378:metabolism; D006801:Humans; D011968:Receptors, Histamine; D012709:Serum Albumin / Q000378:metabolism; D013601:T-Lymphocytes
D005452:Fluoresceins; D011968:Receptors, Histamine; D012709:Serum Albumin; D006632:Histamine