A monoclonal antibody discriminating between subsets of T and B cells.
A description is given of a rat anti-mouse hybridoma antibody, JIId, which reacts with erythrocytes, neutrophils, greater than 90% of thymus cells, and most B cells. JIId does not have detectable activity for mature T cells, pluripotential stem cells, platelets, or cells of the monocyte-macrophage lineage. Although the JIId antigen is present on 90 to 95% of typical small B lymphocytes, pretreatment of spleen cells with JIId plus complement has no effect on secondary IgG antibody responses; by contrast, primary IgM responses and proliferative responses to lipopolysaccharide are substantially reduced. Unlike the precursors of IgG antibody-producing cells (AFC), IgG AFC per se are strongly JIId-positive. Rapid acquisition of the JIId antigen also applied to the early progeny of pluripotential stem cells.
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000145:classification*; D001792:Blood Platelets / Q000276:immunology; D002459:Cell Fusion; D002645:Chickens; D006224:Cricetinae; D004912:Erythrocytes / Q000276:immunology; D005849:Gerbillinae; D006168:Guinea Pigs; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006736:Horses; D006825:Hybridomas / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D009000:Monocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D011817:Rabbits; D051381:Rats; D011917:Rats, Inbred Lew; D012756:Sheep; D013601:T-Lymphocytes / Q000145:classification*
D000911:Antibodies, Monoclonal