Anti-Thy-1-mediated T cell activation. Role of soluble factors and expression of interleukin 2 receptors on T cells.
Rabbit anti-Thy-1 antibody bound to T cells induces the appearance of T cell growth factor (interleukin 2, IL2) receptors and the production of IL2. IL2 receptors induction by this means occurs within a short time span, and independent of any demonstrable accessory cell. A source of exogenous or endogenous IL2 is apparently the only requirement for proliferation after these receptors are expressed. IL2 production, on the other hand, is accessory cell-dependent, and this help is mediated via Fc receptors on the accessory cell. IL2 production in this model is demonstrable at 4-6 h, or earlier, and is proliferation-independent. X-irradiation actually augments apparent IL2 production. The data are consistent with the hypothesis that a differentiated subset of T cells in any mixed population is responsible for IL2 production.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology*; D007376:Interleukin-2 / Q000378:metabolism*; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000494:pharmacology; D011817:Rabbits; D011955:Receptors, Drug / Q000096:biosynthesis; D011971:Receptors, Immunologic; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology*
D000961:Antilymphocyte Serum; D007376:Interleukin-2; D008222:Lymphokines; D008934:Mitogens; D011955:Receptors, Drug; D011971:Receptors, Immunologic