Characterization of three different human T cell membrane antigens, two being present on T lymphocyte subpopulations.
Heteroantisera were raised in rabbits to thymocytes, HSB2 cells, and Sezary cells. Following absorption with Ia-positive leukemia cells, these sera appeared to be specific for different T cell antigens. Both the anti-HSB2 and the anti-Sezary sera reacted with approximately 50% and the antithymocyte serum with 100% of normal peripheral blood T lymphocytes. None of the sera reacted with B cells. The apparent molecular weights of the antigens being detected were determined by immunoprecipitation followed by SDS polyacrylamide gel electrophoresis. A dimer of 170,000 daltons consisting of two similar 85,000-dalton polypeptide chains was immunoprecipitated by the anti-HSB2 serum whereas single polypeptides of 53,000 and 64,000 daltons were immunoprecipitated by the anti-Sezary and antithymocyte sera, respectively.
D000818:Animals; D000954:Antigens, Surface; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology; D011232:Chemical Precipitation; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007945:Leukemia, Lymphoid / Q000276:immunology; D008970:Molecular Weight; D011817:Rabbits; D012751:Sezary Syndrome / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D000954:Antigens, Surface; D000961:Antilymphocyte Serum; D007106:Immune Sera