Fatigue and metabolism of frog muscle fibers during stimulation and in response to caffeine.
Tension and metabolite concentrations were measured in single frog muscle fibers at 15 degrees C in vitro, in response to electrical stimulation or to immersion in caffeine- or potassium chloride-Ringer. Sarcomere length equaled 2.3 micrometers. Interrupted stimulation for 150 s at 20 Hz or stimulation for 7.5 min at 1 Hz was followed by at least 20 min of fatigue, evidenced by a reduced 200-ms test contraction. Fatigued fibers contracted maximally in potassium chloride- or caffeine-Ringer. They had high lactate and glucose 6-phosphate concentrations and a reduced phosphocreatine (PCr) concentration. Adenosine 5'-triphosphate (ATP) concentration was approximately normal but was markedly reduced by a caffeine contracture. A plot of PCr consumption against the tension-time integral at different stimulation frequencies (25, 35, or 50 Hz) and durations had an intercept of 25.5 nmol PCr/mg protein at time zero and a corrected slope of 0.65 nmol approximately P/mg protein per kg . s . cm-2. Prolonged fatigue is not due to energy exhaustion or to the inability of muscle fibers to consume residual ATP but probably arises from long-lasting interference in excitation-contraction coupling, which can be reversed by KCl- or caffeine-induced release of Ca2+ from intracellular stores.
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D004558:Electric Stimulation; D005260:Female; D006003:Glycogen / Q000032:analysis; D007773:Lactates / Q000032:analysis; D019344:Lactic Acid; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009132:Muscles / Q000187:drug effects / Q000502:physiology*; D010725:Phosphocreatine / Q000032:analysis; D011189:Potassium Chloride / Q000494:pharmacology; D011894:Rana pipiens
D007773:Lactates; D010725:Phosphocreatine; D019344:Lactic Acid; D002110:Caffeine; D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D006003:Glycogen