Urinary prostaglandins and kallikrein in the course of acute renal failure.
To examine the role of prostaglandins and the kallikrein system in the recovery from acute renal failure, we studied the sequential changes in urinary prostaglandins and kallikrein after the onset of oliguria. The six patients studied had acute tubular necrosis of the vasomotor type. Urinary PGE2, PGF2 alpha, the PGF2 alpha-main urinary metabolite, 6-keto-PGF1 alpha and TXB2 were all measured by radioimmunoassay. Urinary kallikrein was assayed by means of hydrolytic activity using a chromogenic tripeptide substrate. Following onset of diuresis, urinary PGE2 excretion was increased to normal, parallel to the increase in urine volume. In contrast, the ratio of urinary PGF2 alpha/PGE2 peaked at the onset of diuresis, indicating a relative increase in PGF2 alpha production at this time. Prior to this peak, urinary kallikrein concentrations reached the highest levels, suggesting a close connection with renal prostaglandin metabolism. On the other hand, changes in PGF2 alpha-MUM, 6-keto-PGF1 alpha and TXB2 were not found. These results indicate that there may be an interlocking acute alteration of the kallikrein-prostaglandin system occurring immediately before the resolution of oliguria, although the role of the acute shift to PGF2 alpha production observed needs further study.
D058186:Acute Kidney Injury / Q000652:urine*; D000328:Adult; D000368:Aged; D015237:Dinoprost; D015232:Dinoprostone; D004231:Diuresis; D006801:Humans; D007610:Kallikreins / Q000652:urine*; D007684:Kidney Tubules / Q000473:pathology; D008875:Middle Aged; D009336:Necrosis; D011453:Prostaglandins / Q000652:urine*; D011458:Prostaglandins E / Q000652:urine; D011460:Prostaglandins F / Q000652:urine
D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D015237:Dinoprost; D007610:Kallikreins; D015232:Dinoprostone