The role of T lymphocytes and monocytes in the regulation of human erythropoietic peripheral blood burst forming units.
To clarify the role of T lymphocytes or monocytes in the regulation of human peripheral blood erythroid burst forming unit proliferation, equal numbers of purified T cells, B cells or monocytes were cultured in vitro with null cells in the plasma clot system. BFUE proliferation was directly proportional to the T cell concentration and inversely proportional to monocyte concentration in culture. The effect of B cells was variable and dependent on the extent of T cell contamination. Addition of a highly specific anti-human thymocyte IgG preparation (ATG) to null cells, T cells + null cells or B cells + null cells significantly reduced or eliminated BFUE proliferation. A burst promoting activity (BPA) was found in the supernatants of T cell enriched mixed lymphocyte cultures and could oppose the inhibition of BFUE proliferation by ATG. A burst inhibitory activity was found in the supernatants of monocyte enriched cultures. These studies provide further evidence that BFUE proliferation is determined by opposing actions of stimulator T cells and inhibitor monocytes and/or their soluble products.
D000961:Antilymphocyte Serum / Q000494:pharmacology; D001402:B-Lymphocytes / Q000502:physiology; D002452:Cell Count; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004912:Erythrocytes / Q000166:cytology; D004920:Erythropoiesis; D006801:Humans; D009000:Monocytes / Q000502:physiology*; D013601:T-Lymphocytes / Q000502:physiology*
D000961:Antilymphocyte Serum