[Indicator method of determining the beta-lactamase-inhibiting activity of various compounds].
An indicator for determination of beta-lactamase inhibitory activity of various compounds was developed. The method is based on the direct contact of beta-lactamase with the compounds tested. It excludes the use of test-bacteria and provides recording in the data on the day of the experiment. The indicator method enables the detection of the beta-lactamase inhibitory properties of both beta-lactamase inhibitors and beta-lactam antibiotics, not subjected to destruction by beta-lactamases. The method is likely to be fit for detection of atypical beta-lactams having beta-lactam groups in their molecules (bleomycin group). Antibiotics not belonging to the group of beta-lactams, such as gentamicin, sisomicin, lincomycin and fusidin showed no beta-lactamase inhibitory activity under the conditions of the indicator method. The use of the indicator method provided determination of the inhibitory activity with respect to penicillinase of Bac. licheniformis 749/C in 30 (8.5 per cent) out of 350 fermentation broths of actinomycetes.
D000192:Actinomycetales / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001407:Bacillus / Q000201:enzymology; D019818:Clavulanic Acid; D004789:Enzyme Activation / Q000187:drug effects; D007202:Indicators and Reagents / Q000494:pharmacology*; D008722:Methods; D013997:Time Factors; D065093:beta-Lactamase Inhibitors; D047090:beta-Lactams / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D065093:beta-Lactamase Inhibitors; D047090:beta-Lactams; D019818:Clavulanic Acid
Indikatornyĭ metod opredeleniia beta-laktamazoingibiruiushcheĭ aktivnosti razlichnykh soedineniĭ.