[Determination of active T lymphocytes in the peripheral blood by the AE rosette test. Correlation of the AE and E rosette tests with lymphocyte reactivity in vitro after PHA stimulation].
AE rosette test (Wybran and Fudenberg) detects T lymphocyte subpopulation and correlates with cellular immunity. In this study we found decreased percentage of active T lymphocytes mainly in peripheral blood of newborns, in children with frequent infections and with malabsorption syndrome. We tried to correlate AE and E rosette tests with PHA lymphocyte reactivity in vitro in 231 patients. We found that decreased PHA lymphocytes reactivity better correlates with decreased percentage of active T lymphocytes than with total number of T lymphocytes. Active rosette test (AE) can be usefull in evaluation of cellular immunity in children.
D000328:Adult; D002648:Child; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D007239:Infections / Q000276:immunology; D007958:Leukocyte Count; D008213:Lymphocyte Activation / Q000187:drug effects; D008286:Malabsorption Syndromes / Q000276:immunology; D009369:Neoplasms / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology; D011247:Pregnancy; D012397:Rosette Formation / Q000379:methods*; D013601:T-Lymphocytes / Q000276:immunology*
D010835:Phytohemagglutinins
Oznaczanie limfocytów T aktywnych w krwi obwodowej za pomoca testu rozetkowego AE. Próba korelacji testów rozetkowych AE i E z reaktywnoscia limfocytów in vitro po stymulacji fitohemaglutynina (PHA).