Multiple loci mediate B cell repertoire ontogeny: analysis of the hemagglutinin-specific repertoires in neonatal C.B20 and (C.B20 x B10.D2)F1 mice.
The HA-specific B cell repertoires of 12 to 14-day-old C.B20 and (C.B20 X B10.D2)F1 mice have been characterized and compared to those of BALB/c, B10.D2, and (BALB/c X B10.D2)F1 mice to assess the role of allotype-linked loci in the process of repertoire formation. Both C.B20 and (C.B20 X B10.D2)F1 neonates express repertoires similar to (BALB/c X B10.D2)F1s of the same age, and allotypic analysis indicates that nonparental clonotypes observed in (BALB/c X B10.D2)F1s at this developmental stage are derived predominantly from the B10.D2 parent. In conjunction, these findings indicate that: a) allotype-linked loci, although influential in repertoire formation, clearly interact with loci elsewhere in the genome to shape the expressed repertoire phenotype; b) developmental preference may exist among heterozygous individuals regarding the time at which particular elements of each parental VH haplotype are expressed; and c) the factors that control this preference are dictated by heritable elements unlinked to Ig allotype loci.
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D006388:Hemagglutinins / Q000235:genetics / Q000276:immunology*; D007126:Immunoglobulin Allotypes / Q000235:genetics / Q000276:immunology; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains / Q000235:genetics*
D000911:Antibodies, Monoclonal; D006388:Hemagglutinins; D007126:Immunoglobulin Allotypes