title: Inhibition of lectin-induced lymphocyte activation by 2-cyclohexene-1-one: decreased intracellular glutathione inhibits an early event in the activation sequence.
abstract: The importance of normal intracellular glutathione (GSH) levels in the activation of human peripheral blood lymphocytes (PBL) by mitogenic lectins was explored using 2-cyclohexene-1-one (2-CHX-1), an agent that selectively decreases the levels of intracellular GSH. PBL incubated with mitogenic lectins and graded doses of 2-CHX-1 showed a dose-dependent inhibition of activation assayed by [3H]-thymidine uptake and percentage of blast transformation. 2.5 x 10(-5) M 2-CHX-1, a concentration that decreases GSH levels to less than 20% of control, caused an 80 to 90% suppression of both [3H]-thymidine uptake and blast transformation; lower concentrations were less effective. Time course studies showed that 2-CHX-1 was maximally effective only if added during the first 4 hr of culture. At the concentrations used, 2-CHX-1 was not cytotoxic to lectin-stimulated or unstimulated PBL and did not interfere with mitogen-lymphocyte interaction. These results suggest that adequate levels of glutathione are necessary for lymphocyte activation and that the glutathione requirement is exerted in the early activation sequence.
mesh_terms: D003510:Cyclohexanes / Q000494:pharmacology*; D003512:Cyclohexanones / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D005978:Glutathione / Q000096:biosynthesis / Q000494:pharmacology*; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D007958:Leukocyte Count; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes; D009000:Monocytes / Q000276:immunology; D011952:Receptors, Concanavalin A; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
chemical_list: D003510:Cyclohexanes; D003512:Cyclohexanones; D037102:Lectins; D011952:Receptors, Concanavalin A; D013438:Sulfhydryl Compounds; C028215:2-cyclohexen-1-one; D004247:DNA; D005978:Glutathione