Negatively selected H-2bml and H-2b cells stimulated with vaccinia virus completely discriminate between mutant and wild-type H-2K alleles.
Lymphocyte populations from B6, C-H-2bml (KbmlDb) mutant mice cannot, after both in vivo and in vitro negative selection for alloreactivity, be induced to recognize vaccinia virus presented in the context of H-2Kb. This finding may mean that the T cell receptor(s) expresses a component that is very specific for a particular "active site" on the self-H-2 molecule. Alternatively, (if recognition is directed at a virus-H-2 complex) the more similar 2 H-2 molecules are, the more likely it may be that precursor thymocytes in the mutant with the capacity to bind H-2Kb + vaccinia virus may be deleted during ontogeny as a result of cross-reaction with H-2Kbml + endogenous antigen.
D000483:Alleles; D000818:Animals; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008817:Mice, Mutant Strains; D013601:T-Lymphocytes; D014616:Vaccinia virus
D006183:H-2 Antigens