Anti-ENA antibody in serum determined by ELISA-technique. Description of method and recommended procedure.
An enzyme linked immune sorbent assay, ELISA, for determination of serum antibodies against two different extractable nuclear antigens, the RNAse sensitive ribonucleoprotein (RNP), and the RNAse resistant Sm-antigen is described. The technical variables are systematically evaluated and a recommended procedure selected and described.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000974:Antibodies, Antinuclear; D000941:Antigens; D002417:Cattle; D002467:Cell Nucleus / Q000276:immunology*; D002648:Child; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007185:Incubators; D008875:Middle Aged; D008947:Mixed Connective Tissue Disease / Q000276:immunology*; D012260:Ribonucleases / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
D000974:Antibodies, Antinuclear; D000941:Antigens; C033055:anti-extractable nuclear antigen antibodies; D012260:Ribonucleases