Human IgE, IgG and IgE antibody synthesis in vitro.
Human IgE biosynthesis in vitro was studied by co-culturing T and B cells from atopic patients and normal controls in the presence of 10 microliter/ml pokeweed mitogen for 7 days. IgE and anti-mite IgE ab in the culture media significantly correlated with those in plasma. Normal T cells suppressed in vitro production of IgE and IgE ab significantly more than did atopic T cells but no difference was found in IgG production. The culture of B cells alone (T-depleted fraction) produced IgE equally as well as the co-culture of T and B cells. Anti-mite IgE ab was also produced by B cells alone from mite-sensitive patients. However, IgE biosynthesis in vitro was not consistently affected by pokeweed mitogen. The results suggest a deficiency of the T suppressor system in atopy and the existence of T-independent IgE-producing cells.
D000328:Adult; D000368:Aged; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D006650:Histocompatibility Testing; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology
D007074:Immunoglobulin G; D011043:Pokeweed Mitogens; D007073:Immunoglobulin E