Mitogenic activity of Sindbis virus and its isolated glycoproteins.
Purified preparations of Sindbis virus, a member of the togavirus family, are mitogenic for lymphocytes from a number of different mouse strains. Cell separation techniques, as well as studies using lymphocytes from the congenitally athymic BALB/c nu/nu mouse, showed that Sindbis virus is a T-cell-independent B-cell mitogen. Additionally, the envelope glycoproteins of Sindbis virus, isolated by Triton X-100 extraction and butanol precipitation, stimulated lymphocytes to incorporate five times as much [3H]thymidine into their DNA as did the Sindbis virion. These results are similar to those previously reported for vesicular stomatitis virus and herpes simplex virus types I and II and for the purified glycoproteins of vesicular stomatitis virus and influenza virus.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D008934:Mitogens; D012845:Sindbis Virus / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*
D006023:Glycoproteins; D008934:Mitogens; D014764:Viral Proteins