Two populations of Ia-like molecules on a human B cell line.
Evidence is presented for two populations of Ia-like molecules on the human B cell line Raji. Two monoclonal antibodies, L203 and L227, are described. Both recognize nonpolymorphic determinants on B cell antigens. Both immunoprecipitate 34,000- and 28,000-dalton material from 125I-labeled extracts of Raji, but L227 precipitates 25,000-dalton material as well. Immunodepletion experiments confirm that the antibodies react with two different populations of molecules.
D000906:Antibodies; D001402:B-Lymphocytes / Q000276:immunology*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002460:Cell Line; D011232:Chemical Precipitation; D002999:Clone Cells / Q000276:immunology; D006649:Histocompatibility Antigens / Q000145:classification*; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology; D011863:Radioimmunoassay
D000906:Antibodies; D006649:Histocompatibility Antigens