ANAE staining pattern of rat lymphocytes: lack of correlation with lymphocyte subclasses.
Lymphocytes from various lymphoid organs of WF and BN rats were isolated and stained for acid alpha-naphthyl acetate esterase (ANAE) activity. From all organs examined-peripheral blood, lymph nodes, and spleen-70-80% of the lymphocytes were stained for ANAE activity. After separation into B and non-B cells by affinity chromatography on anti-Ig columns, esterase-negative lymphocytes were detected in both populations, demonstrating that ANAE staining cannot be used as a lymphocyte subclass marker in rats, in contrast to the situation in human and mice.
D000818:Animals; D001402:B-Lymphocytes / Q000145:classification; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002846:Chromatography, Affinity; D006651:Histocytochemistry; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000145:classification / Q000201:enzymology*; D009283:Naphthol AS D Esterase / Q000378:metabolism*; D051381:Rats; D011914:Rats, Inbred BN / Q000276:immunology; D011920:Rats, Inbred WF / Q000276:immunology; D013154:Spleen / Q000166:cytology; D014018:Tissue Distribution
D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases