Assignment of the gene coding for human catalase to the short arm of chromosome 11.
Human and murine catalases can be separated electrophoretically as single bands of different mobility. In man-mouse somatic cell hybrids, however, detection of human catalase is precluded by the complexity of banding patterns resulting from interference of a catalase-modifying enzyme activity. We have identified human catalase in hybrid clones by Laurel electrophoresis employing a specific anti-human catalase antibody, and by exploiting heat stability differences. Catalase co-segregates with LDH A and is probably located on the short arm of chromosome 11.
D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D002374:Catalase / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D002874:Chromosome Mapping; D002906:Chromosomes, Human, 6-12 and X / Q000648:ultrastructure; D004586:Electrophoresis; D006358:Hot Temperature; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology; D051379:Mice; D011489:Protein Denaturation
D000906:Antibodies; D002374:Catalase