Analysis of the response of B cells from CBA/N-defective mice to nonspecific T cell help.
We have investigated the induction of antibody responses to erythrocyte (RBC)-bound antigens in the (CBA/N x B10)F1 mouse. Male B cells, which express the CBA/N defect, were shown to be unresponsive to RBC antigens when the delivered T cell helper activity was solely nonspecific. Thus we demonstrated that defective B cells did not respond to concanavalin A supernatants or bystander helper activity, in spite of the fact that CBA/N-defective mice could produce these T cell activities. The defective B cell did not respond to RBC-bound antigen in the presence of RBC-primed T cells, although the magnitude of this response was usually twofold less than normal controls. The insensitivity of CBA/N defective B cells to nonspecific T cell helper activities seemed to involve at least the inability of RBC antigens to activate defective B cells in the absence of antigen-specific T cell help.
D000818:Animals; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology*; D003208:Concanavalin A / Q000096:biosynthesis / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006736:Horses; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA / Q000235:genetics*; D010527:Perissodactyla; D012729:Sex Chromosome Aberrations / Q000276:immunology*; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology*; D014960:X Chromosome
D000941:Antigens; D003208:Concanavalin A