Enzyme-linked immunosorbent assay for antibodies to native DNA in sera of patients with SLE.
A micro-ELISA technique has been developed to measure antibodies to native DNA and used in SLE patients. The distribution of antibody to native DNA in the main immunoglobulin classes was studied, using anti-human globulin conjugates labelled with peroxidase. the antigen (double-stranded DNA from calf thymus) used in the assay was adsorbed to the surface of polystyrene plates treated with methylated bovine serum albumin. The standardization of the method was carried out by use of globulin calibration curves.
D000327:Adsorption; D000974:Antibodies, Antinuclear / Q000032:analysis*; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D011137:Polystyrenes / Q000494:pharmacology
D000974:Antibodies, Antinuclear; D011137:Polystyrenes; D004247:DNA