Ligand binding to macromolecules: determination of binding parameters by combined use of ligand buffers and flow dialysis; application to calcium-binding proteins.
D000818:Animals; D002021:Buffers; D002135:Calcium-Binding Proteins / Q000032:analysis*; D002147:Calmodulin / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D003956:Dialysis; D006801:Humans; D008024:Ligands; D046911:Macromolecular Substances; D010320:Parvalbumins / Q000032:analysis; D011485:Protein Binding; D011893:Rana esculenta
D002021:Buffers; D002135:Calcium-Binding Proteins; D002147:Calmodulin; D008024:Ligands; D046911:Macromolecular Substances; D010320:Parvalbumins