Identification of ganglio-N-tetraosylceramide as a new cell surface marker for murine natural killer (NK) cells.
The BCG-induced NK cell activity of murine peritoneal exudate cells was abolished by preincubation of effector cells with anti-ganglio-N-tetraosylceramide (anti-asialo GM1) and C but not with other anti-glycolipid antibodies, anti-ganglioside GM1, anti-globoside, and anti-ganglio-N-triosylceramide (anti-asialo GM2). In contrast, the cytotoxic activity of alloimmune T cells was not affected by treatment with anti-asialo GM1 antisera. These findings suggest that asialo GM1 display may be characteristic of NK cell populations and aid in the isolation of this population of cytotoxic cells.
D000818:Animals; D002462:Cell Membrane / Q000276:immunology; D003165:Complement System Proteins; D003429:Cross Reactions; D005677:G(M1) Ganglioside; D005915:Globosides / Q000276:immunology; D006028:Glycosphingolipids / Q000276:immunology*; D007106:Immune Sera / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009163:Mycobacterium bovis / Q000276:immunology; D009374:Neoplasms, Experimental / Q000276:immunology; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology
D005915:Globosides; D006028:Glycosphingolipids; D007106:Immune Sera; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside; D003165:Complement System Proteins