title: The distribution of Helix pomatia lectin receptors on mouse lymphoid cells and other tissues.
abstract: The distribution of receptors for the Helix pomatia lectin on mouse lymphoid cells and other tissues was investigated. Using a sensitive rosetting assay combined with immunofluorescence, lectin receptors were found on the membrane of approximately 90% of peripheral T lymphocytes, 75% of thymocytes, 30% of bone marrow cells, 20% of nude spleen cells, 15-50% of peritoneal exudate macrophages, and a subpopulation of peritoneal exudate mast cells. The Thy-1-positive nude spleen cells were predominantly Helix lectin receptor-negative. Approximately 5% of B lymphocytes were weakly positive, and neutrophils were negative. Receptors were present also on a subpopulation of cells of a fibroblast cell line and in acetone powder from the liver and, at a lower level, from the kidney and brain. Membrane receptors on all cell types were partially detectable without neuraminidase treatment of the cells. Two methods of fractionating Helix lextin-positive cells were employed, which gave significantly different results. By rosetting and depletion using density fractionation, T cell mitogen responses were abolished, while B cell mitogen responses, T cell cytotoxicity, and natural killer cytotoxicity were only slightly affected, if at all, Helix lectin-agarose column fractionation seemed more sensitive, in that essentially all natural kill cells bound to the column, as well as considerable number of B lymphocytes. Cytotoxic T cells were heterogeneous; roughly half were not bound, but the remainder were bound and eluted.
mesh_terms: D000818:Animals; D002449:Cell Aggregation; D002469:Cell Separation; D003602:Cytotoxicity, Immunologic; D006372:Helix, Snails / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008819:Mice, Nude; D011975:Receptors, Mitogen; D012397:Rosette Formation; D012794:Sialic Acids / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D011975:Receptors, Mitogen; D012794:Sialic Acids