[Removal of accompanying yeast fungi from cultures of trichomonad strains (Polymastigina)].
For purifying the cultures of Trichomonas vaginalis and T. hominis from associating yeasts we inoculated them into the upper layer of a viscous medium containing 0.1% agar (Teras, 1955; Tompel, Teras, 1976) poured into a burette. On the 2nd or 3rd day after inoculating the first drops obtained from the lower end of the burette contained as a rule only trichomonads. For purifying the cultures of T. tenax from associating yeasts we prepared a special semisolid medium from the liquid egg medium (Wantland e. a., 1963) by adding agar (0.5%), levorine (50--600 units/1 ccm) and some pieces of solid egg medium (Hallmann, 1953). The curve of an U-tube was filled with this medium and into both branches pure liquid egg medium was poured. In a few days after inoculating the culture of T. tenax with yeasts into one branch of the tube we obtained from the other branch of the tube trichomonads without yeasts.
D002174:Candicidin / Q000494:pharmacology; D003470:Culture Media; D008722:Methods; D014246:Trichomonas vaginalis / Q000302:isolation & purification*; D015003:Yeasts / Q000187:drug effects / Q000302:isolation & purification*
D003470:Culture Media; D002174:Candicidin
Udalenie soputstvuiushchikh drozhzhevykh gribov iz kul'tur shtammov trikhomonad (Polymastigina).