Insulin radioimmunoassay: inappropriately high results obtained using a preprecipitated antibody technique.
A brief comparison of the results of three insulin radioimmunoassay methods indicated that insulin levels measured in heparinised plasma by a pre-precipitated antibody technique were inappropriately high. The error was greatest when low but clinically important levels of the hormone were studied. This effect was due to non-insulin components of the plasma and could be eliminated by the addition of insulin-free plasma to the standard tubes.
D000818:Animals; D000906:Antibodies; D006168:Guinea Pigs / Q000276:immunology; D006493:Heparin; D006801:Humans; D007328:Insulin / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods
D000906:Antibodies; D007328:Insulin; D006493:Heparin