A novel mitogen released by lipid A-stimulated bone marrow cells.
Lipid A induced bone marrow cells derived from lipopolysaccharide responder strain C3H/HeN to release a component to the extracellular fluid that enhanced DNA synthesis of splenocytes derived from the lipopolysaccharide nonresponder strain C3H/HeJ. The mitogenic component was not selected when C3H/HeN splenocytes were used instead of bone marrow. The target cell in splenocyte populations responding to the mitogenic component released by lipid A-stimulated bone marrow cells is a B cell, as judged by the corresponding of individual cells undergoing DNA synthesis determined by autoradiograph and the presence of surface immunoglobulin detected by immunofluorescence. The mitogenic factor is heat-labile, sensitive to trypsin, and intensive to RNase.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001853:Bone Marrow / Q000378:metabolism*; D002448:Cell Adhesion; D002469:Cell Separation; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005260:Female; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000494:pharmacology; D008050:Lipid A / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008222:Lymphokines / Q000096:biosynthesis*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology
D007376:Interleukin-2; D008050:Lipid A; D008070:Lipopolysaccharides; D008222:Lymphokines; D004247:DNA