Helper activity of T lymphocytes which have been stimulated by keyhole limpet haemocyanin in vitro.
Details are given of a system for keyhole limpet haemocyanin (KLH)-induced DNA synthesis by murine T lymphocytes in vitro. Lymph node T cells from mice primed with KLH and Bordetella pertussis were stimulated with KLH under the defined conditions, and it was found that such cultured cells exhibited substantial non-specific helper activity. In contrast similarly primed T cells which had not been cultured showed only antigen-specific help. It is concluded that proper account should be taken of non-specific effects when studying the activity of antigen-specific helper cells in in vitro.
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006433:Hemocyanins / Q000494:pharmacology*; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*
D004247:DNA; D006433:Hemocyanins