Concanavalin A induces a T-cell dependent activation of human tonsil B cells in vitro.
Tonsillar lymphocytes cultured for 7 days in serum-supplemented RPMI-1640 medium were stimulated with Con A or PWM. IgM production was assessed by a hemagglutination inhibition assay. IgM was produced after Con A as well as PWM stimulation. Our data show that T-cell irradiation and/or addition of small numbers of T cells to the responding B-cell population facilitate(s) IgM synthesis. In particular in Con A-stimulated cultures the T-cell number is critical in regulating the appearance of suppressor effects. The results are discussed in terms of the various subpopulations possibly involved in the response to these mitogens.
D001402:B-Lymphocytes / Q000276:immunology; D003208:Concanavalin A / Q000494:pharmacology*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis; D008213:Lymphocyte Activation; D014066:Palatine Tonsil / Q000276:immunology*; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*
D007075:Immunoglobulin M; D011043:Pokeweed Mitogens; D003208:Concanavalin A