title: Disease-related lymphocyte cytotoxicity in rats bearing transitional cell carcinoma of bladder. Effect of immunomodulators.
abstract: Blood lymphocytes from rats bearing transitional cell carcinoma (TCC) of the bladder were studied for their cytotoxicity in vitro against xenogeneic YAC-I target and against syngeneic TCC cells. Control lymphocytes were obtained from age and sex-matched syngeneic rats. The following differences were observed: (1) lymphocytes from TCC-bearing rats were cytotoxic to syngeneic TCC target cells whereas those from control rats were not; (2) lymphocytes from TCC-bearing and control rats were cytotoxic to NK sensitive YAC-I cells; however, NK cells from TCC-bearing rats were more adherent to nylon wool-columns than NK cells from control rats. The adherent and non-adherent cells from TCC-bearing rats were both cytotoxic to syngeneic TCC target cells. Levamisole treatment of effector cells from TCC-bearing rats did not affect the NK activity, yet it increased the cytotoxicity of non-adherent cells on TCC target cells. Treatment of the adherent cells with poly-I:poly-C increased slightly their NK activity on YAC-I cells and their anti-TCC cytotoxicity. However, a marked increase in the cytotoxicity by both adherent and non-adherent cell fractions was observed on TCC target cells pretreated with poly-I:poly-C. A disease-related cytotoxicity of lymphocytes from rats bearing TCC has been observed. Treatment of TCC target cells with poly-I:poly-C increased their susceptibility to lysis by the activated effector cells.
mesh_terms: D000818:Animals; D002295:Carcinoma, Transitional Cell / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D007694:Killer Cells, Natural / Q000276:immunology; D007978:Levamisole / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D009374:Neoplasms, Experimental / Q000276:immunology; D011070:Poly I-C / Q000494:pharmacology; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
chemical_list: D007978:Levamisole; D011070:Poly I-C