Immunological studies on drug-induced allergic hepatitis--hepatocellular injury by macrophage-mediated cytotoxicity.
When the peripheral blood lymphocytes from patients with drug-induced allergic hepatitis were stimulated with a specific drug in vitro in the presence of a liver cytosol fraction containing liver specific antigen, lymphocyte transformation was seen in eight out of 11 patients. The macrophage activating factor (MAF), a kind of lymphokines, was also detectable in the culture medium of activated lymphocytes from seven out of eight patients who showed positive blastogenesis evaluated the uptake of 3H-glucosamine into macrophages. MAF-activated macrophages exhibited a cytotoxic effect on separated liver cells resulting in a marked inhibition of albumin synthesis. This macrophage-mediated cytotoxicity was also observed in eight out of 11 patients who showed positive lymphocyte transformation. These observations suggest that macrophage-mediated cytotoxicity may play some role in the pathogenesis of drug-induced allergic hepatitis.
D000328:Adult; D000368:Aged; D000941:Antigens / Q000032:analysis; D001202:Ascitic Fluid / Q000166:cytology; D056486:Chemical and Drug Induced Liver Injury / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D004342:Drug Hypersensitivity / Q000276:immunology*; D005260:Female; D006801:Humans; D008099:Liver / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D008263:Macrophage Migration-Inhibitory Factors / Q000032:analysis; D008264:Macrophages / Q000276:immunology*; D008297:Male; D008875:Middle Aged
D000941:Antigens; D008263:Macrophage Migration-Inhibitory Factors