Studies on immunoreactive pancreatic elastase 2 in human serum.
A radioimmunoassay for human pancreatic elastase 2 is described. DFP-Inactivated pancreatic elastase 2 is used both as standard and tracer. The concentration in normal human plasma is approximately 60 micrograms immunoreactive pancreatic elastase 2, which rises in acute pancreatitis. Pancreatic elastase 2 appears in plasma in a form with a molecular size exceeding that of the proenzyme. In vitro studies demonstrate that a complex between pancreatic elastase 2 and alpha 1-antitrypsin arises after incubation of a mixture of pancreatic juice and plasma. The results presented support the conclusion that proelastase is bound by alpha 1-antitrypsin after the discharge from the pancreatic gland to the extracellular tissue fluids around the gland.
D002850:Chromatography, Gel; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D010196:Pancreatic Elastase / Q000097:blood*; D010189:Pancreatic Juice / Q000378:metabolism; D010195:Pancreatitis / Q000201:enzymology; D011863:Radioimmunoassay; D000515:alpha 1-Antitrypsin / Q000378:metabolism
D000515:alpha 1-Antitrypsin; D010196:Pancreatic Elastase