Transformation of Rhodopseudomonas sphaeroides with deoxyribonucleic acid isolated from bacteriophage R phi 6P.
The transformation of the photosynthetic bacterium Rhodopseudomonas sphaeroides with the circular genome of the penicillinase-encoding, temperate bacteriophage R phi 6P was demonstrated. The transformation was dependent on the infection of the recipient by another, apparently closely related, temperature bacteriophage, R phi 9. Optimum transformation occurred in the recipient cells already lysogenic for R phi 9 when superinfected with that bacteriophage at multiplicities of infection between 1 and 10 R phi 9 particles per recipient cell.
D001435:Bacteriophages / Q000235:genetics* / Q000502:physiology / Q000528:radiation effects; D004279:DNA, Viral / Q000235:genetics*; D003851:Deoxyribonucleases / Q000494:pharmacology; D007700:Kinetics; D008242:Lysogeny; D012242:Rhodobacter sphaeroides / Q000235:genetics*; D014169:Transformation, Bacterial / Q000187:drug effects; D014466:Ultraviolet Rays
D004279:DNA, Viral; D003851:Deoxyribonucleases