Measurement of mitogen stimulation of lymphocytes with a glucose consumption test.
The increase of glycolysis in mitogen-stimulated lymphocytes was used to quantify lymphoblast transformation. Results correlated well with those by common methods, i.e. morphological evaluation and [3H]thymidine incorporation. The advantage of the glucose consumption test is that it registers the entire event of lymphocyte proliferation whereas other methods evaluate only viable transformed cells at the end of culture.
D003208:Concanavalin A / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007158:Immunologic Techniques; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D008214:Lymphocytes; D008934:Mitogens / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism
D008934:Mitogens; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D003208:Concanavalin A; D005947:Glucose; D013936:Thymidine