Cord IgM antibody specific for human killer T cells: T lymphocytotoxic human fetal antibody (TLFA) recognizing maternal killer T cells proliferating in the presence of interleukin 2.
T lymphocytotoxic human fetal antibody- (TLFA) containing cord IgM (TLFA IgM) was purified from cord blood. The proliferation of maternal mixed lymphocyte culture-(MLC) induced and long-term cultured T cells (LCT) specific to the paternal non-T cells in the presence of interleukin 2 (IL 2) was inhibited by treatment with the respective TLFA IgM plus complement (C). Sixty-six to 78% of Leu-2a-positive maternal LCT were stained with the respective TLFA IgM, as determined by anti-Leu antibodies and TLFA IgM double-staining. Moreover, TLFA IgM plus C treatment reduced the killer activity of the respective maternal LCT against the PHA-induced paternal target cells. Therefore, TLFA-positive killer T cells of maternal LCT belong to certain killer T cell subsets of Leu-2a-positive pan-killer T cells, and TLFA contains a potential antibody against maternal killer T cells.
D000918:Antibody Specificity; D000961:Antilymphocyte Serum / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D005260:Female; D005312:Fetal Blood / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis; D007231:Infant, Newborn; D007376:Interleukin-2 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D013601:T-Lymphocytes / Q000276:immunology
D000961:Antilymphocyte Serum; D007075:Immunoglobulin M; D007376:Interleukin-2