Determination of ionization constants by paper electrophoresis.
Dimensionless apparent ionization constants of charged low-molecular-weight species may be obtained from paper-electrophoretic data at 20-25 degrees C with buffers (I0.1-0.5) of measured pH (1.5-12.5) containing oxalate ions. Relative mobilities rather than absolute mobilities were measured by using glycerol and m-nitrobenzenesulphonate respectively as standards of zero and unit mobility. Application of the procedure to ionizations of adenine, adenosine, 2'-deoxyadenosine, 3'-deoxyadenosine, 3':5'-cyclic AMP, ADP, ADP-glucose-agrocin 84 and ATP is described.
D000225:Adenine; D000227:Adenine Nucleotides; D000241:Adenosine; D001430:Bacteriocins; D002021:Buffers; D003839:Deoxyadenosines; D004590:Electrophoresis, Paper / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical
D000227:Adenine Nucleotides; D001430:Bacteriocins; D002021:Buffers; D003839:Deoxyadenosines; C013799:agrocin 84; D000225:Adenine; D000241:Adenosine