Haemoglobin content of multilayer E rosettes is a parameter of activated human T lymphocytes.
In stimulated cultures, a proportion of human T lymphocytes form multilayer rosettes with sheep red blood cells (SRBC) and they can be easily separated from common E rosettes by velocity sedimentation. We have developed a simplified method for harvesting multilayer rosettes and quantifying them. Practical applications of this technique are discussed.
D002478:Cells, Cultured; D002498:Centrifugation; D004247:DNA / Q000096:biosynthesis; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation; D055095:Optics and Photonics; D012397:Rosette Formation / Q000379:methods; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D006454:Hemoglobins; D004247:DNA