Metabolism of adenosine and 2'-deoxy-adenosine by fetal mouse calvaria in culture.
The metabolism of endogenous adenosine and 2-deoxy-adenosine was studied in cultures of fetal mouse calvaria. Adenosine deamination was the most important pathway of metabolism. This was blocked by erythro-2-(2-hydroxy-3-nonyl)adenine (1 microM). Albumin in the medium could not account for the deaminase activity. The disappearance of adenosine from the medium was not influenced by two inhibitors of adenosine transport, dipyridamole and dilazep, but was competitively inhibited by 2-deoxy-adenosine. During culture there was a net increase in adenosine and inosine, possibly originating from damaged cells.
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000241:Adenosine / Q000378:metabolism*; D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D003470:Culture Media; D003839:Deoxyadenosines / Q000378:metabolism*; D004109:Dilazep / Q000494:pharmacology; D004176:Dipyridamole / Q000494:pharmacology; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism; D007288:Inosine / Q000378:metabolism; D051379:Mice; D009924:Organ Culture Techniques
D003470:Culture Media; D003839:Deoxyadenosines; D007042:Hypoxanthines; D019271:Hypoxanthine; C010192:9-(2-hydroxy-3-nonyl)adenine; D007288:Inosine; D004176:Dipyridamole; D004109:Dilazep; D000225:Adenine; D000241:Adenosine