Membrane fluorescent probes for revealing lymphocyte population heterogeneity. II. T-and B-lymphocytes of human peripheral blood.
Lymphocytes of human peripheral blood were stained with a fluorescent probe 3-methoxybenzanthrone (MBA) and their fluorescence intensities measured using a cytofluorimeter. The lymphocytes are shown to be heterogeneous in fluorescence intensity. The fluorescence intensity of B-cells is 2-3 times higher than that of T-cells. Some data suggest that null-cells have an intermediate fluorescence intensity. Thus, with MBA it is possible to distinguish T- and B-lymphocytes in man.
D000328:Adult; D001402:B-Lymphocytes; D001551:Benz(a)Anthracenes / Q000494:pharmacology*; D002469:Cell Separation; D005456:Fluorescent Dyes / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000145:classification*; D012397:Rosette Formation; D013601:T-Lymphocytes
D001551:Benz(a)Anthracenes; D005456:Fluorescent Dyes; C024290:3-methoxybenzanthrone