title: Histochemical determination of estrogen and progesterone binding in fine needle aspirates of breast cancer. Correlation with conventional biochemical assays.
abstract: Estrogen and progesterone binding sites were assayed in patients with primary, recurrent and metastatic mammary carcinoma using a new histochemical technique. This histochemical assay employed fluorescein-labeled conjugates of bovine serum albumin covalently bound to estradiol or progesterone. In a double-blind study in which the results of histochemistry were correlated with those obtained by a conventional biochemical assay, there was 83% correlation for estrogen binding in 41 cases and 87% correlation for progesterone binding in 31 cases.
mesh_terms: D001665:Binding Sites; D001707:Biopsy, Needle; D001943:Breast Neoplasms / Q000378:metabolism*; D002466:Cell Nucleolus / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004311:Double-Blind Method; D004967:Estrogens / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D011374:Progesterone / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*
chemical_list: D004967:Estrogens; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D011374:Progesterone