Donor cell leukemia after bone marrow transplantation in the Friend leukemia in mice.
After lethal irradiation (800 R) Friend virus (FV-P)-infected leukemic DBA/2 mice were transplanted with normal bone marrow cells. Isogeneic transplantation led to an immediate relapse of leukemia. Therefore, allogeneic bone marrow cells were taken from almost FV-P resistant C57BL/6 mice. A measure of leukemia development was given by the number of erythropoietin-independent erythroid colonies (CFU-EI) in bone marrow and spleen, characteristic for the Friend leukemia. Even after allogeneic transplantation leukemia recurred after 5 to 19 days. By an electrophoretic analysis of the hemoglobin, it could be shown that the transformed erythropoiesis was donor derived. Thus, marrow of C57BL/6 origin loses its FV-P resistance in allogeneic leukemic lethally irradiated recipients and is transformed by the surviving virus.
D000818:Animals; D001797:Blood Protein Electrophoresis; D016026:Bone Marrow Transplantation; D003114:Colony-Forming Units Assay; D004920:Erythropoiesis; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D006918:Hydroxyurea / Q000494:pharmacology; D007942:Leukemia, Experimental / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D012156:Reticulocytes; D013163:Splenomegaly / Q000276:immunology; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic; D014965:X-Rays
D004921:Erythropoietin; D006918:Hydroxyurea