The preparation of protoplasts from Aspergillus fumigatus mycelium.
Protoplasts have been prepared from the mycelium of Aspergillus funigatus, using a lytic enzyme mixture from Trichoderma harzianum. A variety of experimental conditions were investigated in order to achieve optimal conditions for protoplast production. Electron micrographs showed the preparations to be free of cell-wall. Material obtained by this procedure can be used as a source of cytoplasmic antigens for further analysis.
D001232:Aspergillus fumigatus / Q000648:ultrastructure*; D002458:Cell Fractionation; D008854:Microscopy, Electron; D011523:Protoplasts / Q000648:ultrastructure*; D014242:Trichoderma / Q000201:enzymology