[Effect of colchicine on the in vitro maturation of frog oocytes as affected by a suspension of hypophysis and progesterone].
The influence of colchicine (250 micrograms/ml) on in vitro oocyte maturation in the frog Rana temporaria was studied. The maturation of oocytes was induced by treatment of the follicles with pituitary suspension or progesterone. The gonadotropin-dependent period upon oocyte maturation in all studied females was shortened under the effect of colchicine. The dose of progesterone required for 50% GVBD in the colchicine-treated follicles of 4 out of 11 females was lower than in the intact follicles, in the other cases the dose was the same. Colchicine did not influence the time of 50% GVBD.
D000818:Animals; D003078:Colchicine / Q000494:pharmacology*; D005260:Female; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development; D010063:Ovum / Q000187:drug effects*; D010902:Pituitary Gland; D011374:Progesterone / Q000494:pharmacology*; D011896:Rana temporaria; D013535:Suspensions; D013997:Time Factors; D014020:Tissue Extracts / Q000494:pharmacology*
D013535:Suspensions; D014020:Tissue Extracts; D011374:Progesterone; D003078:Colchicine
Vliianie kolkhitsina na sozrevanie in vitro ootsitov travianoĭ liagushki pod deĭstviem suspenzii gipofizov i progesterona.