title: Regulatory role of IgE-binding factors from rat T lymphocytes. IV. Formation of IgE-binding factors in rats treated with complete Freund's adjuvant.
abstract: Mesenteric lymph node (MLN) cells from rats treated with complete Freund's adjuvant (CFA) spontaneously released soluble factors, which have affinity for IgE (IgE-binding factors). The factors were purified by absorption of culture filtrates of the lymphocytes with IgE-Sepharose followed by elution at acid pH. Purified IgE-binding factors selectively suppressed IgE-forming cell response of DNP-ovalbumin-primed MLN cells to homologous antigen without affecting the IgG2 response. Fractionation of purified IgE-binding factors on lentil lectin-Sepharose showed that the majority of IgE-binding factors did not have affinity for lentil lectin, whereas a minor fraction was bound to the lectin-coupled beads. It was found that IgE-binding factors lacking affinity for lentil lectin suppressed IgE response, whereas those eluted from lentil lectin-Sepharose selectively enhanced IgE response. Serum of CFA-treated animals also contained both IgE-suppressive factor and IgE-potentiating factor, and the amount of the former factor was more than the latter. The source of IgE-suppressive factor is non-B lymphocytes. Depletion of Fc, receptor(+) lymphocytes in MLN cells from CFA-treated animals did not affect the formation of IgE-suppressive factor, indicating that Fc epsilon R-bearing cells are not the source of the factor.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D004136:Dinitrobenzenes / Q000276:immunology; D005620:Freund's Adjuvant / Q000494:pharmacology*; D007073:Immunoglobulin E; D007165:Immunosuppression Therapy; D008198:Lymph Nodes / Q000276:immunology; D008970:Molecular Weight; D010047:Ovalbumin / Q000276:immunology; D011817:Rabbits; D051381:Rats; D011917:Rats, Inbred Lew; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D004136:Dinitrobenzenes; D007073:Immunoglobulin E; D010047:Ovalbumin; D005620:Freund's Adjuvant