Characteristics of C3H/He mouse embryo cell lines established by culture with or without benzaldehyde.
The characteristics of RME-5-3-1 cell line, which had been established from C3H/He mouse embryos by culture in benzaldehyde-containing medium, were compared with those of a benzaldehyde-untreated cell line, RME-5-1, derived from the same embryos as the former and with those of RME-5-1/TMT cell line, reestablished from the tumors induced by implantation of RME-5-1 cells into syngeneic mice. The characterization of these cell lines covered cell morphology, chromosome distribution, population doubling time, saturation density, fibronectin, epidermal growth factor receptor, plasminogen activator, ornithine decarboxylase, anchorage-independent growth and transplantability into mice. The results indicated that RME-5-3-1 cells had well-preserved normal phenotypes, while both RME-5-1 and RME-5-1/TMT cells showed malignant phenotypes to varying degrees.
D000818:Animals; D001547:Benzaldehydes / Q000494:pharmacology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003470:Culture Media; D004622:Embryo, Mammalian; D004815:Epidermal Growth Factor; D005353:Fibronectins / Q000032:analysis; D007621:Karyotyping; D008722:Methods; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011955:Receptors, Drug / Q000032:analysis
D001547:Benzaldehydes; D003470:Culture Media; D005353:Fibronectins; D011955:Receptors, Drug; D004815:Epidermal Growth Factor; D009955:Ornithine Decarboxylase