A trypsin method for coating human red blood cells with plasma C3 and/or C4.
Simple procedures are described for the production of human red blood cells coated with C3 and/or C4 by brief trypsin treatment of cell-plasma mixtures at room temperature. These cells ('EC3/4'-try) were unusual in that their agglutinability by anticomplement sera, as compared to sucrose-water RBC, was similar when tested by AutoAnalyzer but was markedly reduced in manual testing, especially against anti-C4. In spite of this, both 'EC34'-try and 'EC3'-try were found to be suitable indicator cells for determining the adequacy of anti-C3c and/or anti-C3d activities in reagents used for manual antiglobulin tests.
D000888:Antibodies, Anti-Idiotypic; D003176:Complement C3; D003181:Complement C4; D004912:Erythrocytes / Q000276:immunology*; D006384:Hemagglutination; D006801:Humans; D013696:Temperature; D014357:Trypsin / Q000494:pharmacology*; D014361:Trypsin Inhibitors / Q000494:pharmacology
D000888:Antibodies, Anti-Idiotypic; D003176:Complement C3; D003181:Complement C4; D014361:Trypsin Inhibitors; D014357:Trypsin