Effect of mouse anti-I region antiserum and complement on human mononuclear cell response to concanavalin A.
Pretreatment of human peripheral blood mononuclear cells with mouse anti-Ia serum and complement was found to inhibit the subsequent T cell proliferative response to Con A. The cells from each individual tested were inhibited, regardless of DR allospecificity. Inhibition of Con A response by mouse anti-Ia antibodies and C was found to be due to elimination of adherent accessory cells (monocytes) rather than T cells. Anti-Ia serum appeared to recognize an essential subpopulation of human monocytes bearing cross-reactive antigens, since not all monocytes treated with anti-Ia serum and C were lysed. The use of highly restricted mouse anti-Ia serum demonstrated that the required monocyte population expressed antigens recognized by antibodies with activity to I-Ek products (Ia specificities Ia.7 and Ia.22). The required monocyte subpopulation was also recognized by a monoclonal antibody specific for Ia.7. Mouse anti-I-Ek alloantiserum or monoclonal anti-Ia.7 antibodies may be important tools with which to study human monocytes, and for further characterization of DR determinant requirements for monocyte-antigen presentation and monocyte-T cell interactions.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000961:Antilymphocyte Serum / Q000494:pharmacology*; D002448:Cell Adhesion; D003165:Complement System Proteins / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008805:Mice, Inbred A; D009000:Monocytes / Q000276:immunology; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000961:Antilymphocyte Serum; D000949:Histocompatibility Antigens Class II; D007166:Immunosuppressive Agents; D003208:Concanavalin A; D003165:Complement System Proteins