Radioimmunoassay and chromatographic characterisation of CRF-41-like immunoreactivity in hypothalami from several species.
We have developed a sensitive and specific radioimmunoassay (RIA) for the 41-residue ovine corticotropin releasing factor (CRF), employing direct radioiodination of the non-tyrosine containing synthetic peptide. Two different antisera are described, both raised against synthetic CRF conjugated to bovine thyroglobulin. With these RIA's, CRF-like immunoreactivity (CRF-LI) which eluted in the same position as synthetic ovine CRF on Sephadex G-50 was detected in ovine, bovine and rat stalk median eminence (SME). An additional, later-eluting peak of CRF-LI was found only in rat hypothalamic extracts. CRF-LI could also be detected in the void region of the chromatograms in all three species.
D000818:Animals; D000906:Antibodies; D002417:Cattle; D002850:Chromatography, Gel; D003346:Corticotropin-Releasing Hormone / Q000032:analysis* / Q000276:immunology; D003429:Cross Reactions; D005260:Female; D007031:Hypothalamus / Q000032:analysis*; D008473:Median Eminence / Q000032:analysis; D010455:Peptides / Q000032:analysis* / Q000276:immunology; D011863:Radioimmunoassay; D051381:Rats; D012756:Sheep; D013045:Species Specificity
D000906:Antibodies; D010455:Peptides; D003346:Corticotropin-Releasing Hormone