Macrophage T-cell interaction in man: handling of tetanus toxoid antigen by human monocytes.
An absolute requirement for monocytes was demonstrated in the T-cell proliferative response to tetanus toxoid (TT) antigen. Antigen-pulsed monocytes were shown to be effective in triggering T-cell proliferation. Using 125I-radiolabeled TT antigen, uptake by monocytes increased progressively over an 18-hr period, at which time 80-85% of the monocytes contained radiolabeled material. The ability of antigen-pulsed monocytes to trigger T-cell proliferation paralleled antigen uptake over an 18-hr period. Monocytes pulsed with antigen, then washed, lost their ability to trigger T-cell proliferation following a 24- to 48-hr culture period. Metabolic inhibitors blocked antigen uptake by monocytes and monocyte triggering of T-cell proliferation. Trypsin treatment of TT-pulsed monocytes did not affect the amount of antigen associated with monocytes or T-cell triggering by monocytes. Anti HLA-DR alloantibodies, which when added during antigen pulsing of monocytes inhibit the capacity of these monocytes to trigger T-cell proliferation, did not interfere with antigen uptake. These results indicate that human monocytes present antigen to T cells via an active process and in association with Dr determinants, and that the immunogenic moiety of antigen does not remain indefinitely available to the T cell.
D000941:Antigens; D002450:Cell Communication; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology*; D014357:Trypsin / Q000494:pharmacology
D000941:Antigens; D000949:Histocompatibility Antigens Class II; D007106:Immune Sera; D013745:Tetanus Toxoid; D003571:Cytochalasin B; D014357:Trypsin; D003078:Colchicine