Glucagon and glicentin immunoreactivity are topologically segregated in the alpha granule of the human pancreatic A cell.
Glicentin is a 100-amino acid polypeptide purified from the porcine intestine and containing the immunodeterminants of glucagon. We have previously reported the presence of glicentin immunoreactivity in the glucagon-containing, specific secretory granules (alpha granules) of the pancreatic and gastric A cell. With an improved immunocytochemical probe, the protein A-gold (pAg) technique, we are now able to show that glucagon and glicentin-like material are topologically segregated in the alpha granule of the human pancreatic A cell.
D005934:Glucagon / Q000031:analogs & derivatives / Q000276:immunology / Q000378:metabolism*; D006046:Gold; D006801:Humans; D007158:Immunologic Techniques; D007515:Islets of Langerhans / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*
D010455:Peptides; D006046:Gold; D005934:Glucagon