Regulation of the synthesis of S-100 protein in rat glial cells.
The influence of serum on the synthesis of the nervous system specific S-100 protein has been investigated in the rat glial cell clone C61a. In sparse cells, where S-100 synthesis is low, removal of serum leads to a 2- to 3-fold increase of the rate of S-100 synthesis relative to total soluble cell protein. Measurements of S-100 content by radioimmunoassay showed that sparse cells are accumulating S-100 much more rapidly in the absence of serum. Our findings suggest that serum components inhibit the expression of S-100 synthesis in sparse cells by a concentration-dependent mechanism. This effect does not correlate directly with the reduced cellular growth rate in low serum or serum-free medium. Lipid-free medium, which has little growth-promoting activity, maintains the capacity to lower the rate of S-100 synthesis. A partially purified extract of platelets can substitute for serum in repressing the synthesis of the S-100 protein.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009457:Neuroglia; D014176:Protein Biosynthesis / Q000187:drug effects; D051381:Rats; D009418:S100 Proteins / Q000096:biosynthesis*; D014168:Transferrin / Q000494:pharmacology
D007328:Insulin; D009419:Nerve Tissue Proteins; D009418:S100 Proteins; D014168:Transferrin