[Correlation between preincubation time of the leukocyte feeder-layer and in vitro granulocytic-macrophage colony growth].
Granulocytic-macrophage colony in vitro formation depends on the presence of the colony stimulating factor (CSF). When, in double layer agar method, the CSF is provided from leucocyte underlayers, the pre-incubation time of the feeder-layers must be kept constant. The results obtained in this study have demonstrated in fact that the growth of the colonies can be remarkably different if feeder-layers are incubated for 1, 7 or 14 days.
D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D006098:Granulocytes / Q000502:physiology*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008264:Macrophages / Q000502:physiology*; D013997:Time Factors
D003115:Colony-Stimulating Factors
Rapporti tra tempo di preincubazione del feeder-layer leucocitario e crescita in vitro di colonie granulocitiche-macrofagiche.