Studies on nude mice bearing human CSF-producing tumor. III. Colony type of granulocyte-macrophage precursors of nude mice bearing CSF-producing tumor.
In previous studies, we analyzed the hemopoietic precursor cells of tumor-bearing nude mice with marked neutrophilia induced by a transplanted human lung cancer. In this study, we examined the morphology of granulocyte-macrophage colonies using a technique for cytochemical examination of all colonies grown in agar gel culture. By stimulation with spleen cell-conditioned medium, the bone marrow cells and spleen cells of tumor-bearing nude mice formed a higher proportion of granulocyte-containing colonies than those of control mice. However, a larger number of granulocyte-macrophage progenitors were not definitely committed to differentiate into granulocytes and macrophages, as no significant differences in cellular composition of colonies between tumor-bearing mice and control mice were observed by stimulation with L cell-conditioned medium.
D000818:Animals; D001854:Bone Marrow Cells; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000502:physiology*; D003470:Culture Media; D006098:Granulocytes / Q000166:cytology*; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007700:Kinetics; D007739:L Cells; D008175:Lung Neoplasms; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013154:Spleen / Q000166:cytology
D003115:Colony-Stimulating Factors; D003470:Culture Media