An immunoenzyme assay for quantitation of human IgG antibodies to honeybee venom phospholipase A2.
A new method to measure the concentrations of IgG antibodies to phospholipase A2 in sera from patients treated with honeybee venom immunotherapy is described. This method utilizes a microcentrifugal analyzer to detect inhibition of PLA2 enzymatic activity by antibodies in serum standards and unknowns. Sera from beekeepers with known concentrations of specific antibodies, measured by radioimmunoprecipitation, were used to construct a logit-log standard curve for the immunoenzyme assay. The standard curve was linear for concentrations between 2.3 and 20.0 microgram/ml. The concentrations of specific antibodies measured by enzyme inhibition correlated well with the concentrations as measured by radioimmunoprecipitation, r = 0.959 (least squares linear regression), n = 32. Interassay variation was 10.1% at 10 microgram/ml. The immunoenzyme method is rapid and does not require radiolabeled reagents.
D000906:Antibodies / Q000032:analysis*; D001514:Bee Venoms / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology*; D010741:Phospholipases A / Q000276:immunology; D054467:Phospholipases A2; D014688:Venoms / Q000201:enzymology
D000906:Antibodies; D001514:Bee Venoms; D007074:Immunoglobulin G; D014688:Venoms; D010741:Phospholipases A; D054467:Phospholipases A2