Cell-mediated lympholysis responses against autologous cells modified with haptenic sulfhydryl reagents. II. Analysis of the genetic control and H-2-restricted pattern of cytotoxic responses to sulfhydryl and amino reactive reagents.
N-iodoacetyl-N'-(5-sulfonic-1-naphthyl)ethylene-diamine modified autologous cells are shown to induce respective high and low AED-self CTL responses by spleen cells from H-2b and H-2k mice. The lysis mediated by effector populations from both haplotypes was highly H-2 restricted. Cytotoxic responses induced by a 2nd sulfhydryl- (SH) specific reagent, dithionitrobenzoic acid (DTNB), were shown not to be cross-reactive at the effector cell level with AED-self but exhibited the same high and low CTL response patterns as those induced against AED-self. These Ir-effects reported for the 2 SH reactive compounds are discussed in relation to the opposite Ir-patterns demonstrated against a number of amino (NH2) reactive compounds. The difference of self-recognition sites is suggested to account for the contrasting results observed in the response patterns induced by SH- and NH2-reactive compounds. The AED-self CTL response was found to exhibit Kk-self over Db-self Ir regulation. Thus, this latter type of genetic control appears to function independently of whether the hapten is an SH- or NH2-reactive compound.
D000818:Animals; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D004228:Dithionitrobenzoic Acid / Q000276:immunology; D006183:H-2 Antigens / Q000235:genetics*; D006241:Haptens; D007460:Iodoacetamide / Q000031:analogs & derivatives / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009282:Naphthalenesulfonates / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013439:Sulfhydryl Reagents / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D006183:H-2 Antigens; D006241:Haptens; D009282:Naphthalenesulfonates; D013439:Sulfhydryl Reagents; D014301:Trinitrobenzenes; C006421:1,5-I-AEDANS; D004228:Dithionitrobenzoic Acid; D007460:Iodoacetamide