In vivo and in vitro effect of the Haemophilus influenzae lipopolysaccharide on ciliated respiratory epithelium.
The lipopolysaccharide of Haemophilus influenzae is presumed to have a toxic effect on the tracheal epithelium, and then induce a bronchial obstruction. This activity of LPS was studied in vitro on the ciliary beat using a photo-oscillographic apparatus, and in vivo on the rabbit trachea. Neither modification of ciliary beat frequency, nor epithelial damage in the rabbit trachea was observed after a single administration of LPS. In contrast, histopathologic changes were observed in vivo when the intratracheal administration of H. influenzae LPS was followed 24 h later by an intravenous injection of the same LPS. These experimental models seem thus to implicate a Shwartzman type cellular necrosis in the trachea in vivo in the absence of a direct toxic effect of endotoxin itself on trachea in vivo or in vitro.
D000818:Animals; D002923:Cilia / Q000502:physiology*; D004848:Epithelium / Q000502:physiology; D006193:Haemophilus influenzae / Q000032:analysis*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009068:Movement / Q000187:drug effects; D011817:Rabbits; D051381:Rats; D014132:Trachea / Q000187:drug effects / Q000502:physiology*
D008070:Lipopolysaccharides