title: Cell lines derived from a human myelomonocytic leukaemia.
abstract: Two cell lines have been isolated from separate samples, taken at the same time, from the peripheral blood of a case of myelomonocytic leukaemia. One of these (CESS-B) is a typical B cell line obtained after treatment of the peripheral blood white cells with Epstein-Barr virus (EBV). The second line (RC-2) is a monocyte-macrophage type, originally isolated by 5 months continuous culture in the presence of colony stimulating factor (CSF). Subsequently, after further culture, this line has become autonomous (RC-2A), no longer requiring CSF for maintenance of proliferation, whilst retaining the characteristics of the original line.
mesh_terms: D000328:Adult; D001773:Blood Cells / Q000473:pathology; D001853:Bone Marrow / Q000473:pathology; D002460:Cell Line; D002999:Clone Cells; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors; D006801:Humans; D007951:Leukemia, Myeloid / Q000473:pathology*; D008297:Male; D010100:Oxygen; D013997:Time Factors
chemical_list: D003115:Colony-Stimulating Factors; D010100:Oxygen