title: Effects of metabolic inhibitors on the regulation of pancreatic glucagon release.
abstract: The effects of metabolic inhibition on the kinetics of glucagon release by pancreatic islets from normal guinea pigs have been studied in a perfusion system. All three metabolic inhibitors, malonate, iodoacetate and 2,4-dinitrophenol, induced a marked stimulation of glucagon secretion, each displaying its own characteristic pattern of response. This was accompanied by a rapid and marked decrease in the ATP concentration of the A2-cell as evidenced by measurements performed in A2-cell rich islets, isolated from guinea pigs treated with streptozotocin. The ATP levels were reduced by about 90% following iodoacetate and 2,4-dinitrophenol exposure and by about 60% after exposure to malonate. The data support the hypothesis that the inhibition of glucagon release from the A2-cell is regulated via an intracellular, energy-dependent mechanism.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D004140:Dinitrophenols / Q000494:pharmacology*; D005934:Glucagon / Q000378:metabolism*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007461:Iodoacetates / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008314:Malonates / Q000494:pharmacology*
chemical_list: D004140:Dinitrophenols; D007461:Iodoacetates; D008314:Malonates; D000255:Adenosine Triphosphate; D005934:Glucagon