Taenia solium: mitogenic effect of larval extracts on murine B lymphocytes.
The effect of an extract (CE) obtained from Cysticercus cellulosae on the proliferation of lymphocytes was studied in cultures of murine spleen cells. The addition of CE to the cultures resulted in the highly significant uptake and incorporation of tritiated thymidine (3H-TdR) into DNA. This phenomena was dose-dependent, with doses lower and higher than the optimal concentration causing less marked effects. The kinetic peak of this response was found to occur on day 2 of culture. CE evoked a proliferative response in cultures of spleen cells from congenitally athymic (nu/nu) BALB/c mice. Cultures of bone marrow-derived (B) lymphocytes, generated by treatment of spleen cells with rabbit antithymocyte serum and complement, incorporated 3H-TdR to a degree similar to that of normal spleen cell cultures. CE did not induce the proliferation of thymocytes. To eliminate the possibility that the mitogenic effect of CE was due to LPS, we carried out experiments using Polymyxin B (PB). CE was mitogenic after treatment with PB which inactivated the LPD effect. In addition, CE elicited 3H-TdR uptake in spleen cells from the LPS nonresponsive C3H/HeJ mouse strain.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D003552:Cysticercus / Q000276:immunology*; D004247:DNA / Q000096:biosynthesis; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D011112:Polymyxin B / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology; D013621:Taenia / Q000276:immunology*
D004247:DNA; D011112:Polymyxin B