[Changes in the detectability of HLA antigens on lymphocyte subpopulations induced by mitogens and corticosteroids].
A study was made of changes in the detectability of HLA-A, HLA-B and HLA-DR histocompatibility antigens on subpopulations of immunocompetent cells under the effect of mitogens and corticosteroids. HLA-antigens were identified by the lymphocytotoxic test. It was found that cell cultivation and mitogen stimulation lead to the fact that distinct reaction with the cells is produced by a greater number of HLA-antisera, while the specificity of direct and cross reactions remains unchanged. The main contribution to changes in the detectability of HLA-antigens is made by T cells. The exposure of the cells to dexamethasone in a dose producing no cytotoxic effect drastically lowered cell proliferation under the effect of PHA and deteriorated the detectability of HLA-A and HLA-B antigens. As regards HLA-DR antigens, it was disclosed that PM-stimulation of B cells considerably decreased the expression of their antigens, whereas PHA-stimulation of T cells led to the detection of HLA-DR antigens on these cells.
D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D008934:Mitogens / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D006680:HLA Antigens; D008934:Mitogens; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D003907:Dexamethasone
Izmenenie vyiavliaemosti HLA antigenov na subpopuliatsiiakh limfotsitov pod vliianiem mitogenov i kortikosteroidov.