Epidermal growth factor requirement for development of cultured mammary gland.
The mouse mammary gland in serum-free whole organ culture can be manipulated hormonally to undergo one complete physiological cycle consisting of lobuloalveolar development, functional differentiation and regression, mimicking processes that occur in vivo. A second cycle has not previously been achieved in vitro. The present study has identified a specific requirement for epidermal growth factor (EGF) in the morphological development of mammary lobuloalveoli, allowing two complete cycles of development and regression in culture.
D000818:Animals; D002454:Cell Differentiation; D003470:Culture Media; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D006728:Hormones / Q000494:pharmacology; D008321:Mammary Glands, Animal / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D051379:Mice; D009924:Organ Culture Techniques; D010455:Peptides / Q000494:pharmacology*
D003470:Culture Media; D006728:Hormones; D010455:Peptides; D004815:Epidermal Growth Factor