Evaluation of a low-ionic-strength solution-monospecific anti-IgG antiglobulin technique for donor antibody screening.
Three different techniques of antibody screening of donor bloods were sequentially evaluated. Group I (16,300 donors) was a standard saline-albumin-AHG technique utilizing polyvalent serum, including incubation at room temperature. In Group II (26,243 donors), incubations (including room temperature) were performed in LISS, and monovalent anti-IgG serum was used. For Group III (15,840 donors), the room temperature incubation was not used for the LISS-IgG method of Group II. The three methods were comparable in terms of detection of clinically significant antibodies, while in Group III the detection of clinically nonsignificant antibodies was eliminated. Cost analysis indicates that for a donor center processing approximately 50,000 units per year and willing to prepare its own LISS solutions, conversion to LISS-IgG could produce a savings of between $5,000 and $8,000 per year. LISS-IgG is thus a sensitive and economical technique highly recommended for the donor center committed to manual donor antibody screening.
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000918:Antibody Specificity; D001782:Blood Donors; D006801:Humans; D007074:Immunoglobulin G; D009994:Osmolar Concentration; D012709:Serum Albumin; D012965:Sodium Chloride; D013696:Temperature
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D012709:Serum Albumin; D012965:Sodium Chloride