The effect of nitrofurantoin on deoxyribonucleic acid synthesis in plant mitochondria.
Nitrofurantoin (1-([(5-nitrofuran-2-yl)methylene]amino)imidazolidine-2,4-dione), a widely used drug and also a well-known bacterial mutagen, inhibits DNA synthesis in mitochondria from 48 h etiolated seedlings of Vigna sinensis (Linn.) Savi (snake bean). The effect appears at the level of the uptake of radioactive deoxynucleoside triphosphates by the plant mitochondria. Nitrofurantoin does not inhibit DNA synthesis in vitro by homogeneous Escherichia coli DNA polymerase I and DNA polymerase from avian-myeloblastosis virus. No specific nitroreductase activity could be detected in mitochondria.
D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D004247:DNA / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003854:Deoxyribonucleotides / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D007887:Fabaceae / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D009582:Nitrofurantoin / Q000494:pharmacology*; D010944:Plants / Q000187:drug effects / Q000378:metabolism*; D010946:Plants, Medicinal
D003854:Deoxyribonucleotides; D004247:DNA; D009582:Nitrofurantoin; D004259:DNA-Directed DNA Polymerase