Selective suppression of the major idiotypic component of an antihapten response by soluble T cell-derived factors with idiotypic or anti-idiotypic receptors.
Evidence is presented for the selective suppression of the major idiotypic component of the humoral response to the phenylarsonate hapten by soluble factors derived from T cells (TsF). The existence of TsF with anti-idiotypic receptors was also demonstrated. It was found that TsF with idiotypic and anti-idiotypic receptors coexist in cultures of spleen cells prepared from idiotypically suppressed, hyperimmunized mice. By gel filtration the molecular weight of each factor was found to be 50,000-100,000. Each is sensitive to trypsin and is bound to a column containing anti-H-2a antibodies. Evidence is discussed which suggests the possibility of mutual stimulation of suppressor T cells with idiotypic and anti-idiotypic receptors.
D000818:Animals; D000917:Antibody Formation; D001665:Binding Sites; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D006183:H-2 Antigens / Q000032:analysis; D006241:Haptens; D007108:Immune Tolerance; D007130:Immunoglobulin Idiotypes; D008222:Lymphokines / Q000276:immunology*; D051379:Mice; D011971:Receptors, Immunologic; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D006183:H-2 Antigens; D006241:Haptens; D007130:Immunoglobulin Idiotypes; D008222:Lymphokines; D011971:Receptors, Immunologic