Cytochrome P-450-dependent O-dealkylase activity in mammalian skin.
1 A modified technique for the measurement of O-dealkylase (ODA) activity in crude homogenates is reported, and its application to skin is described. 2 Large differences in ODA levels were found between different species, no activity being observed in the skin of primates. 3 Induction of cutaneous ODA in mice was achieved by the subcutaneous injection of phenobarbitone, hexachlorobenzene or 20-methylcholanthrene. 4 Attempts to induce ODA in vitro were unsuccessful.
D015660:7-Alkoxycoumarin O-Dealkylase; D000293:Adolescent; D000328:Adult; D000818:Animals; D002417:Cattle; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D006581:Hexachlorobenzene / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008253:Macaca mulatta; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D051379:Mice; D008812:Mice, Hairless; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D010105:Oxygenases / Q000032:analysis / Q000096:biosynthesis*; D010634:Phenobarbital / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D012867:Skin / Q000201:enzymology*; D013045:Species Specificity
D006581:Hexachlorobenzene; D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; D015660:7-Alkoxycoumarin O-Dealkylase; D010634:Phenobarbital