Synthesis of [2-11C]5,5-dimethyl-2,4-oxazolidinedione for studies with positron tomography.
We have developed a method for the synthesis of [2-11C]5,5-dimethyl-2,4-oxazolidinedione ([2-11C]DMO) for use with positron emission tomography to measure regional tissue pH in vivo in man. [2-11C]Dimethyl carbonate (DMC) was prepared from [C-11]phosgene and excess of sodium methoxide in methanol containing dimethyl carbonate as added carrier. The [2-11C]DMC solution was then reacted with 2-hydroxyisobutyramide at 150 degrees C for 10 min to yield, after HPLC separation, [2-11C]DMO with a radiochemical yield of 20-56%. Chemical yields were 78-92%, and specific activity ranged as high as 830 mCi/millimol.
D001921:Brain / Q000000981:diagnostic imaging; D002250:Carbon Radioisotopes; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004114:Dimethadione / Q000138:chemical synthesis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007553:Isotope Labeling; D010080:Oxazoles / Q000138:chemical synthesis*; D010705:Phosgene / Q000138:chemical synthesis; D014055:Tomography, Emission-Computed
D002250:Carbon Radioisotopes; D010080:Oxazoles; D010705:Phosgene; D004114:Dimethadione