Transformation of glicentin-containing L-cells into glucagon-containing cells by enzymatic digestion.
Exposure of sections of ileal mucosa to enzymatic digestion with trypsin and carboxypeptidase B reveals a population of immunofluorescent cells after incubation with a specific C-terminally directed antiglucagon serum. These cells, unreactive before enzyme treatment, were identified as L-cells by their immunoreactivity to antiglicentin serum and to cross-reacting (N-terminal) antiglucagon sera. The presence in the L-cells of antigenic sites characteristic of the glucagon-containing cells (A-cells) emphasizes the close relationships between these two cell types, and it further supports the hypothesis of glicentin as a glucagon precursor.
D000818:Animals; D002268:Carboxypeptidases; D004285:Dogs; D005455:Fluorescent Antibody Technique; D005768:Gastrointestinal Hormones / Q000032:analysis*; D005934:Glucagon / Q000096:biosynthesis* / Q000276:immunology; D007082:Ileum / Q000166:cytology* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology* / Q000378:metabolism; D010455:Peptides / Q000032:analysis*; D014357:Trypsin
D005768:Gastrointestinal Hormones; D010455:Peptides; D005934:Glucagon; D002268:Carboxypeptidases; D014357:Trypsin