title: E-rosette inhibitory factor in sera from patients with mycosis fungoides.
abstract: Peripheral blood lymphocytes from some of patients with mycosis fungoides disease showed decreased ability to form rosettes with sheep erythrocytes. This decreased percentage of E-rosette forming cells could be normalized when those cells were incubated in culture for 20 hr. Since these data led us to considering a possible inhibitory factor present in patients' sera, we tested their ability to inhibit E-rosetting by T lymphocytes from normal donors, and found that sera from mycosis fungoides patients with low levels of E-rosetting blood lymphocytes showed greater inhibitory effect on E-rosette formation by normal T cells when compared to those either from normal donors or from mycosis patients who had almost normal levels of E-rosetting blood lymphocyte number. The E-rosette inhibitory factor was sensitive to 2-mercaptoethanol treatment and was copurified with serum IgM by ammonium sulfate precipitation and by sequential gel filtrations, suggesting that it might be an anti-T lymphocyte antibody naturally occurring during the disease process.
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000961:Antilymphocyte Serum / Q000302:isolation & purification; D001798:Blood Proteins / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008077:Lipoproteins, LDL; D008297:Male; D008623:Mercaptoethanol / Q000494:pharmacology; D008875:Middle Aged; D009182:Mycosis Fungoides / Q000276:immunology*; D012397:Rosette Formation; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000961:Antilymphocyte Serum; D001798:Blood Proteins; D008077:Lipoproteins, LDL; C022060:rosette-inhibiting factor; D008623:Mercaptoethanol