Failure to detect papovavirus-associated T antigens in human brain tumor cells by anticomplement immunofluorescence.
To probe the possible presence of papovavirus-related T antigen(s) in human brain tumors, the imprinted or cultured cells at various passage levels were examined by anticomplement immunofluorescence using antisera to T antigen of each BK virus, JC virus, and simian virus 40. No T antigen was demonstrated in any tests with cells derived from 69 patients with various brain tumors. Twenty-two tumor cell strains cultured in the presence of a tumor promoter, phorbol ester, also failed to show the T antigen.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D001932:Brain Neoplasms / Q000276:immunology*; D002478:Cells, Cultured / Q000187:drug effects; D003165:Complement System Proteins; D006801:Humans; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D027383:Papillomaviridae / Q000276:immunology; D027382:Polyomaviridae; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014412:Tumor Virus Infections / Q000276:immunology*
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D003165:Complement System Proteins; D013755:Tetradecanoylphorbol Acetate