title: The promoter-proximal region of the Bacillus licheniformis penicillinase gene: Nucleotide sequence and predicted leader peptide sequence.
abstract: Penicillinase (beta-lactamase) is a major species of secreted protein produced by Bacillus licheniformis 749. From the pTB2 recombinant plasmid containing the cloned entire penicillinase (penP) gene, we have isolated and sequenced a 446-bp HpaII fragment carrying the beginning of penP. The 3'-end coding region of 216-bp on this DNA fragment codes for the first 72 amino acids of the prepenicillinase protein. The deduced structure of the leader peptide consists of a 34 amino acid signal sequence with a hydrophilic N-terminal region and a central hydrophobic core.
mesh_terms: D001407:Bacillus / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D004274:DNA, Recombinant / Q000032:analysis; D005815:Genetic Code; D009876:Operon; D010405:Penicillinase / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D004274:DNA, Recombinant; D010405:Penicillinase; D001618:beta-Lactamases