Elevation of C-peptide immunoreactivity in plasma of normal subjects after trypsin action.
After measuring the effects of trypsin on the plasma of forty-five fasting healthy subjects, a very large increase in C-peptide immunoreactivity was observed. Determination of the proinsulin concentration by gel filtration showed that this increase could not be solely imputed to conversion of circulating proinsulin into desalanyl-insulin and C-peptide by trypsin. Consequently, it is suggested that certain plasma proteins structurally similar to C-peptide might be released by the action of trypsin and crossreact in the radioimmunoassay.
D002096:C-Peptide / Q000097:blood*; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood; D008297:Male; D010455:Peptides / Q000097:blood*; D011384:Proinsulin / Q000097:blood; D011863:Radioimmunoassay; D014357:Trypsin
D002096:C-Peptide; D007328:Insulin; D010455:Peptides; D011384:Proinsulin; D014357:Trypsin