title: Alteration of clonal profile. III. T15 ontogenetic advantages are not sufficient for establishing idiotypic dominance in adoptive transfer.
abstract: We have examined the ontogeny of BALB/c plaque-forming cell (PFC) responses to phosphorylcholine (PC) from fetal and neonatal liver by using the (CBA/N x BALB/c)F1 transplantation model. In this system, thymus-dependent (PC-keyhole limpet hemocyanin) and thymus-independent class 1 (PC-Brucella abortus, PC-lipopolysaccharide) PC antigens stimulate B cell subpopulations, which functionally emerge early after transfer. Responsiveness to a thymus-independent class 2 antigen, C-polysaccharide extract of a Streptococcus pneumoniae mutant, is acquired later. The response to PC antigens tested initially exhibited T15 dominance. Non-T15 clones, which are not expressed to a great degree in normal BALB/c mice, are inherently slow in their rate of maturation; in adoptive transfer, however, they eventually comprise much of the transplanted anti-PC PFC response. Obviously, the advantages the T15 subset has in ontogeny do not result in idiotypic dominance once the immature cells are removed from the intact BALB/c environment. We discuss possible regulatory mechanisms involved in the alteration of the T15+:T15- ratio.
mesh_terms: D000818:Animals; D002999:Clone Cells / Q000276:immunology; D005260:Female; D005333:Fetus / Q000276:immunology; D005799:Genes, Dominant; D007116:Immunization, Passive; D007130:Immunoglobulin Idiotypes / Q000235:genetics / Q000276:immunology*; D007153:Immunologic Deficiency Syndromes / Q000276:immunology; D007165:Immunosuppression Therapy; D008099:Liver / Q000166:cytology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics / Q000276:immunology*; D008808:Mice, Inbred CBA / Q000235:genetics / Q000276:immunology*; D010767:Phosphorylcholine / Q000276:immunology; D011247:Pregnancy; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology
chemical_list: D007130:Immunoglobulin Idiotypes; D010767:Phosphorylcholine