The influence of pH on the survival after X-irradiation of cultured malignant cells. Effects of carbonylcyanide-3-chlorophenylhydrazone.
Irradiation of cultured murine mammary carcinoma (M 8013 S) cells during incubation in buffers of different pH showed a decrease in radiation sensitivity with lower pH. The presence of CCCP (carbonylcyanide-3-chlorophenylhydrazone) during incubation had no effect at pH values above 7.5, but at lower pH there was a marked increase in radiation sensitivity. The effects of CCCP are presumably related to its proton-conducting properties.
D000818:Animals; D001943:Breast Neoplasms; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects*; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006863:Hydrogen-Ion Concentration; D051379:Mice; D009374:Neoplasms, Experimental; D009570:Nitriles / Q000494:pharmacology*; D011837:Radiation-Protective Agents; D011878:Radiotherapy / Q000379:methods; D014965:X-Rays
D009570:Nitriles; D011837:Radiation-Protective Agents; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone