Stimulation of the in vivo dinitrophenyl antibody response to the DNP conjugate of L-glutamic acid60-L-alanine30-L-Tyrosine10 (GAT) polymer by a synthetic adjuvant, muramyl dipeptide (MDP): target cells for adjuvant activity and isotypic pattern of MDP-stimulated response.
Specific anti-dinitrophenyl (DNP) response to DNP-conjugated L-glutamine60-L-alanine30-L-tyrosine10 (DNP-GAT) was obtained in GAT-responder mice by using synthetic N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) as adjuvant. Significant levels of anti-DNP antibodies were observed during a secondary response to DNP-GAT, when both antigen and MDP were used for priming. In this system, MDP was able to prime the carrier-specific T cells but not the hapten specific B cells. The study of the isotypic pattern of the anti-DNP response shows that MDP stimulates only the appearance of specific anti-DNP IgG1 plaque-forming cells. Anti-DNP plaque-forming cells were stimulated in animals primed with DNP-GAT in Freund's complete adjuvant or in Maalox-pertussis and used as control IgG1, IgG2a, and IgG2b.
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology*; D000276:Adjuvants, Immunologic; D000409:Alanine / Q000276:immunology; D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D004136:Dinitrobenzenes / Q000276:immunology*; D005260:Female; D005971:Glutamates / Q000276:immunology; D006020:Glycopeptides / Q000494:pharmacology*; D007116:Immunization, Passive; D007156:Immunologic Memory; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D009578:Nitrobenzenes / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology; D010567:Pertussis Vaccine / Q000276:immunology; D011108:Polymers; D013601:T-Lymphocytes / Q000276:immunology; D014443:Tyrosine / Q000276:immunology
D000276:Adjuvants, Immunologic; D004136:Dinitrobenzenes; D005971:Glutamates; D006020:Glycopeptides; D009578:Nitrobenzenes; D010567:Pertussis Vaccine; D011108:Polymers; C020749:GAT; D014443:Tyrosine; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D010047:Ovalbumin; D000409:Alanine