Induction of murine B cell proliferation by insolubilized anti-immunoglobulins.
This study demonstrates that intact antibodies specific for mu, delta, or light chains when coupled to Sepharose are effective in delivering proliferative signals to B cells. Furthermore, using Sepharose-coupled anti-delta antibodies, we have shown that hybridoma anti-delta (that is not active as soluble protein) is as effective as rabbit anti-delta in activating murine B cells. However, antibodies directed against two other surface molecules, I-A and H-2K, were not mitogenic. Thus, sIgM and sIgD are comparable in their ability to transmit a proliferative signal to the B cell and sIg seems to play a unique role in this regard.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000166:cytology*; D002455:Cell Division; D005260:Female; D006822:Hybrid Cells / Q000276:immunology; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000494:pharmacology; D011817:Rabbits; D012995:Solubility
D000888:Antibodies, Anti-Idiotypic; D008934:Mitogens