[State of the contractile apparatus during the development of a pathological process in the muscles. V. The effect of Zenker's necrosis and of denervation atrophy on F-actin structure].
By means of polarized UV fluorescent microscopy, the state of F-actin was studied in single glycerinized muscle fibers from intact, locally damaged and denervated m. semi-tendinosus of the frog. It was shown that F-actin of denervated muscle fiber lost the ability to reply by increasing tryptophan fluorescence anisotropy during the fiber relaxation and its stretching in the rigor solution by 1--4 per cent compared to the original length. Zenker's necrosis retains this ability only slightly. It is supposed that both the denervation atrophy and Zenker's necrosis change the structure of F-actin.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D003714:Denervation; D005454:Fluorescence Polarization; D066298:In Vitro Techniques; D008856:Microscopy, Fluorescence; D008859:Microscopy, Polarization; D008860:Microscopy, Ultraviolet; D009124:Muscle Proteins / Q000378:metabolism*; D009126:Muscle Relaxation; D009133:Muscular Atrophy / Q000378:metabolism*; D009336:Necrosis; D011896:Rana temporaria
D000199:Actins; D009124:Muscle Proteins
Izuchenie sostoianiia sokratitel'noigo apparata pri razvitii patologicheskogo protsessa v myshtsakh. V. Vliianie tsenkerovskogo nekroza i denervatsionnoĭ atrofii na strukturu F-aktina.