Some characteristics of a new multiple-channel photometer for through-the-plate reading of microplates to be used in enzyme-linked immunosorbent assay.
The performance of a prototype of a now commercially available multiple-channel photometer for high-speed reading of microplates to be used in the enzyme-linked immunosorbent assay was evaluated. For this purpose the extinction of a stable test liquid dispensed in flat-bottomed polystyrene microplates was measured, varying the test volume and the optical density. From the results obtained for dilutions of a single, biolgoically inactive material we concluded that the photometric inaccuracy is negligible when performing enzyme-linked immunosorbent assay with biologically active samples.
D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D010783:Photometry / Q000295:instrumentation*