Nonspecific alpha-naphthyl acetate esterase activity of T-lymphocytes: study in healthy newborns and children, in immune deficiencies and juvenile rheumatoid arthritis.
The aim of this study was to compare the E rosette-forming cells and nonspecific alpha naphthyl esterase (ANAE)-positive lymphocyte values in normal and pathologic situations. In newborns, the ANAE-positive lymphocytes represented less than 60% of the E rosette population. During the first year of life, E rosette-forming cells (E-RFC) reached normal values as soon as one month whereas only three-fourths of the T cells exhibited an ANAE-positive staining. In adult T cell populations, nearly 90% were ANAE-positive. Our observations of immune deficiencies suggested that the relative proportions of E-RFC and ANAE-positive lymphocytes were generally comparable to normal values. However, in the majority of the patients with very low or absent E-RFC (severe combined immune deficiencies, Di George syndrome, and congenital rubella), some ANAE-positive lymphocytes could be detected. Our immunologic survey shows that the ANAE-positive lymphocytes were in a normal range 2 years after a bone marrow transplantation in severe combined immune deficiencies patients. One child who exhibited a normal amount of E-RFC and whose lymphocytes failed to respond in vitro to mitogens had practically no ANAE-positive lymphocytes. An elevated amount of ANAE-positive cells in juvenile rheumatoid arthritis may reflect an augmentation of the T helper functions which permanently stimulated in vivo immunoglobulin production.
D000293:Adolescent; D000328:Adult; D001172:Arthritis, Rheumatoid / Q000097:blood*; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000097:blood*; D007223:Infant; D007231:Infant, Newborn; D009283:Naphthol AS D Esterase / Q000097:blood*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000201:enzymology*
D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases