Studies on the sub-banding of creatine kinase MM and the "CK conversion factor".
In serum, the M subunit of creatine kinase (CK) isoenzymes, MM and MB, is modified by a heat labile factor. As a result of the modification three sub-bands of MM and two of MB can be demonstrated by agarose electrophoresis. Normal sera contain MM1 as the dominant sub-band with lesser amounts of MM2 and MM3. Following tissue damage the MM sub-band distribution changes as a result of the increased release of MM, which occurs as MM3. Immediately after coronary bypass surgery MM3 is the dominant sub-band to be replaced by MM2 and in turn by MM1. The factor responsible for the modification of the M subunit, the "CK conversion factor" has been partially purified and its molecular weight determined.
D001688:Biological Products / Q000302:isolation & purification* / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D001026:Coronary Artery Bypass; D003402:Creatine Kinase / Q000097:blood*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007527:Isoenzymes; D008970:Molecular Weight; D009206:Myocardium / Q000201:enzymology
D001688:Biological Products; D007527:Isoenzymes; D003402:Creatine Kinase