Intratumor maturational heterogeneity within the murine myeloma MOPC-315.
The murine myeloma MOPC-315 secretes a paraprotein which binds dinitrophenylated compounds. Maturational subsets which mimic normal B-cell differentiation were shown to exist within this monoclonal neoplasm. The maturational subsets were defined by an in vivo stem cell activity (plasmacytoma colony-forming unit-spleen), DNA synthesis ([3H]thymidine incorporation), membrane-bound paraprotein, and cells secreting the MOPC-315 paraprotein. Velocity sedimentation at unit gravity separated cells enriched for secreting the MOPC0-315 protein (14.6 mm/hr) from the plasmacytoma colony-forming unit-spleen enriched population (9.3 mm/hr). An in vivo sequential analysis of the appearance of tumor (i.v. challenge) in the spleen of BALB/c hosts did not reveal any discordant appearance of these same maturational subsets with respect to time. The MOPC-315 myeloma contains maturational subsets that mimic normal B-cell differentiation and apparently differentiates during a very early stage of its evolution within the host.
D000818:Animals; D001402:B-Lymphocytes / Q000473:pathology; D002454:Cell Differentiation; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006412:Hematopoietic Stem Cells / Q000473:pathology; D051379:Mice; D009194:Myeloma Proteins / Q000378:metabolism; D010954:Plasmacytoma / Q000473:pathology*; D013154:Spleen / Q000473:pathology*
D004273:DNA, Neoplasm; D009194:Myeloma Proteins