The role of anti-H-2K and H-2D alloantibodies in enhancement and acute antibody-mediated rejection of mouse skin allografts.
The enhancing and destructive effects of anti-H-2Kd and anti-H-2Dk antibodies were studied in B6AF1 recipients of B10.D2 or B10.BR skin allografts. The anti-H-2 antibodies were isolated from B6AF1 anti-B10.D2 and B6AF1 anti-B10.BR alloantiserum by adsorption to donor strain red blood cells and subsequent elution. Acute antibody-mediated rejection of B10.D2 or B10.BR skin grafts was readily induced by administration of anti-H-2K or H-2D antibodies, respectively, along with rabbit complement. Treatment of the B6AF1 recipients with the antibody eluates on day 0, 2, and 4 after transplantation resulted in specific and significant prolongation of graft survival. Both graft destruction and enhancement can therefore be mediated by antibodies directed against the same H-2 specificity.
D000208:Acute Disease; D000818:Animals; D003165:Complement System Proteins; D005260:Female; D006082:Graft Enhancement, Immunologic; D006084:Graft Rejection; D006085:Graft Survival; D006183:H-2 Antigens / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007518:Isoantibodies / Q000276:immunology*; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011817:Rabbits; D016038:Skin Transplantation; D014184:Transplantation, Homologous
D006183:H-2 Antigens; D007074:Immunoglobulin G; D007518:Isoantibodies; D003165:Complement System Proteins