[Determination of T- and B-lymphocyte counts in guinea pigs].
T lymphocytes of guinea-pigs were found to form spontaneous E-rosettes with rabbit red blood cells. The lymphocyte count constituted 32% of blood lymphocytes, 41% of lymph node cells. Meanwhile no T lymphocytes were detected in the bone marrow. The T lymphocyte subpopulation capable of forming "early" rosettes was demonstrated. B lymphocytes formed EAC-rosettes with sheep red blood cells. The B lymphocyte count in the blood, lymph nodes and bone marrow amounted to 10, 18 and 5%, respectively. The effects of incubation conditions, centrifugation, antibody types, and complement concentration, etc. on E- and EAC-rosette formation were defined.
D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D001854:Bone Marrow Cells; D004912:Erythrocytes / Q000276:immunology; D006168:Guinea Pigs; D007958:Leukocyte Count / Q000379:methods*; D008198:Lymph Nodes / Q000166:cytology; D011817:Rabbits; D012397:Rosette Formation; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology
Opredelenie kolichestva T- i B-limfotsitov u morskikh svinok.