Growth stimulation of hamster embryonic fibroblasts by phorbol esters.
The growth on hamster embryonic fibroblasts was highly dependent on the serum concentration in the medium. A tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), enhanced the growth of hamster embryonic fibroblasts in the medium containing low concentrations of serum. Among phorbol derivatives, phorbol-12,13-didecanoate and 4-O-methyl-12-O-tetradecanoyl-phorbol-13-acetate also showed growth stimulation, but 4 alpha-phorbol-12,13-didecanoate, phorbol-12,13-diacetate and phorbol did not. TPA increased the accumulation of 2-deoxyglucose into the cells at low serum concentrations. Thus, hamster embryonic fibroblasts were sensitive to phorbol esters as growth stimulators, and TPA reduced the serum requirement of the cells.
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D006224:Cricetinae; D003470:Culture Media; D003847:Deoxyglucose / Q000378:metabolism; D004622:Embryo, Mammalian; D004815:Epidermal Growth Factor / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D008647:Mesocricetus; D010704:Phorbols / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D003470:Culture Media; D010704:Phorbols; D004815:Epidermal Growth Factor; D003847:Deoxyglucose; D013755:Tetradecanoylphorbol Acetate