Fluoride resistant alpha-naphthyl acetate esterase and combined enzyme histochemistry in the study of normal and pathologic lymphoid tissues.
Alpha-Naphthyl acetate esterase (ANAE) and fluoride resistant alpha-naphthyl acetate esterase (FRANAE) have been compared as histochemical methods to identify T lymphocytes in sections of normal and pathological human lymphoid tissues. In addition, the FRANAE method was combined with alkaline phosphatase (ALP) in order to simultaneously evaluate the relationship between T lymphocytes and fibroblastic reticular cells (ALP) positive). The "dot like" esterase positivity of T lymphocyte was better evaluated by using FRANAE when compared to ANAE because of fluoride inhibitor of the strong esterase activity of dendritic cells and most macrophages. The combined ALP-FRANAE method clearly demonstrated a large number of fibroblastic reticular cells within the T-areas in various normal and pathological tissues such as hyperplastic lymph nodes and especially in the lymph nodes and spleens from patients with Hodgkin's disease.
D000469:Alkaline Phosphatase / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D004351:Drug Resistance; D005459:Fluorides / Q000494:pharmacology; D006651:Histocytochemistry; D006689:Hodgkin Disease / Q000473:pathology; D006801:Humans; D008198:Lymph Nodes / Q000166:cytology; D008221:Lymphoid Tissue / Q000166:cytology*; D008223:Lymphoma / Q000473:pathology; D009283:Naphthol AS D Esterase / Q000378:metabolism*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000201:enzymology*
D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases; D000469:Alkaline Phosphatase; D005459:Fluorides