Cell fusion-impaired variants of a mouse lymphocytic cell line obtained by single-step selection for polyethylene glycol resistance.
This report describes an effective method for single-step isolation of cell line variants with reduced intercellular plasma membrane fusion ability. Variants, derived from a subline of L5178Y, were obtained by selection for growth in the presence of a toxic level of PEG 6000. These variants occurred after EMS treatment and spontaneously. Further testing revealed a pleiotropic phenotype, fusion impairment and polyethylene glycol resistance, which has been designated PgfR. Variant sublines, in serial subculture for more than 165 days on nonselective medium, retain a stable PgfR phenotype. PgfR variants have an approximate 7-fold reduction in intercellular fusion competence.
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002999:Clone Cells / Q000187:drug effects; D004351:Drug Resistance; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005821:Genetic Techniques; D014644:Genetic Variation; D008214:Lymphocytes; D051379:Mice; D009153:Mutagens; D011092:Polyethylene Glycols / Q000494:pharmacology*
D009153:Mutagens; D011092:Polyethylene Glycols; D005020:Ethyl Methanesulfonate