A computer-assisted spectrophotometric enzyme assay. A method for the calculation of Km and V from a single reaction progress curve by the integrated form of the Lineweaver-Burke plot with particular reference to beta-lactamase.
D001407:Bacillus / Q000201:enzymology; D003201:Computers; D006868:Hydrolysis; D007700:Kinetics; D008722:Methods; D010400:Penicillin G; D013056:Spectrophotometry, Ultraviolet; D001618:beta-Lactamases
D001618:beta-Lactamases; D010400:Penicillin G