title: 111Indium-labeled human alveolar macrophages and monocytes: function and ultrastructure.
abstract: Human alveolar macrophages and peripheral blood mononuclear cells were labelled with indium-111 (111In)-oxine in ethanol. The efficiency of labeling averaged 84% for macrophages and 74% for mononuclear cell preparations. Phagocytosis by macrophages, the release of colony stimulating factor activity (CSF) by macrophages, and chemotaxis by monocytes in response to human C5-derived chemotactic factor (C5fr) and formyl-methionyl-leucyl-phenylalanine (f-MLP), were indistinguishable between labeled and unlabeled cells. Microscopically, labeled cells looked similar to control cells. Radioautography demonstrated 111In throughout the labeled cells, but there was a disproportionately high quantity over nuclei. These findings add to the growing literature indicating that 111In labels cells efficiently and that cells can retain function after being labeled with 111In.
mesh_terms: D001345:Autoradiography; D002634:Chemotaxis, Leukocyte; D003115:Colony-Stimulating Factors / Q000378:metabolism; D006801:Humans; D007204:Indium; D008264:Macrophages / Q000502:physiology* / Q000648:ultrastructure; D009000:Monocytes / Q000502:physiology* / Q000648:ultrastructure; D010587:Phagocytosis; D011650:Pulmonary Alveoli / Q000166:cytology*; D011868:Radioisotopes
chemical_list: D003115:Colony-Stimulating Factors; D011868:Radioisotopes; D007204:Indium