Comparison of a receptor binding assay with a radioimmunoassay for measuring human epidermal growth factor-urogastrone in urine.
A radioreceptor assay for human epidermal growth factor-urogastrone (EGF-URO) employing mouse EGF-URO as tracer and calibration standards and human placental receptor as binding protein is compared with a radioimmunoassay for human EGF-URO. Results employing the receptor assay indicated similar values for urinary concentrations (1-16 nmol/l) for healthy control subjects and for patients with psoriasis. Lower values were obtained for bed-ridden patients with burns or patients recovering from orthopaedic surgery. The results obtained by radioimmunoassay were somewhat higher and correlated poorly (r = 0.5) with those obtained with the radioreceptor assay. The reason for this discrepancy is discussed.
D000328:Adult; D000368:Aged; D002056:Burns / Q000652:urine; D004815:Epidermal Growth Factor / Q000652:urine*; D005069:Evaluation Studies as Topic; D006801:Humans; D007103:Immobilization; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000652:urine; D011863:Radioimmunoassay; D011869:Radioligand Assay
D004815:Epidermal Growth Factor