Cell kinetics of lithium-induced granulopoiesis.
Lithium has been shown to be an effective inducer of granulopoiesis. The mechanism of lithium action has been shown to influence CFU-s and CFU-c proliferation, increase colony-stimulating factor (CSF) production and reduce erythropoiesis. We report here evidence to show that lithium recruits CFU-c that are not normally in the cell cycle into active DNA synthesis, as measured by hydroxyurea and tritiated thymidine suicide techniques. Furthermore, lithium action is shown to be time-dependent, since the delay addition of lithium for 5 min to normal bone marrow removes the enhancement usually seen when lithium is given at time zero. The implications of these lithium-induced effects are described.
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006098:Granulocytes / Q000502:physiology*; D006410:Hematopoiesis / Q000187:drug effects*; D006918:Hydroxyurea / Q000494:pharmacology; D008094:Lithium / Q000494:pharmacology*; D051379:Mice; D013936:Thymidine / Q000494:pharmacology; D013997:Time Factors
D003115:Colony-Stimulating Factors; D004247:DNA; D008094:Lithium; D013936:Thymidine; D006918:Hydroxyurea