title: Anti-ENA antibody in serum determined by ELISA-technique. Description of method and recommended procedure.
abstract: An enzyme linked immune sorbent assay, ELISA, for determination of serum antibodies against two different extractable nuclear antigens, the RNAse sensitive ribonucleoprotein (RNP), and the RNAse resistant Sm-antigen is described. The technical variables are systematically evaluated and a recommended procedure selected and described.
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000974:Antibodies, Antinuclear; D000941:Antigens; D002417:Cattle; D002467:Cell Nucleus / Q000276:immunology*; D002648:Child; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007185:Incubators; D008875:Middle Aged; D008947:Mixed Connective Tissue Disease / Q000276:immunology*; D012260:Ribonucleases / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
chemical_list: D000974:Antibodies, Antinuclear; D000941:Antigens; C033055:anti-extractable nuclear antigen antibodies; D012260:Ribonucleases