Isolation and characterization of an R-prime plasmid from Rhizobium meliloti.
Using a simple enrichment procedure, we isolated an R-prime derivative of plasmid R68.45 carrying a 17.8-megadalton segment of the Rhizobium meliloti 41 chromosome. The chromosomal segment carried on this plasmid (pGY1) includes the markers cys-24+, cys-46+, and att16-3. Plasmid pGY1 mobilized the chromosome in a polarized way starting from the region of homology, but cannot promote chromosome transfer from other sites. The att16-3 site on pGY1 allowed the integration of phage 16-3 into pGY1, and a composite plasmid of 91.8 megadaltons was formed. This vector (pGY2) is suitable for the introduction of Rhizobium bacteriophage 16-3 into other gram-negative bacteria.
D001435:Bacteriophages; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D011815:R Factors; D011995:Recombination, Genetic; D012231:Rhizobium / Q000235:genetics*
D004269:DNA, Bacterial