Role of adenosine deaminase in the early stages of precursor T cell maturation.
The role of adenosine deaminase in the initial stages of precursor T cell differentiation was examined in patients with adenosine deaminase deficiency. Incubation of precursor T cells from adenosine deaminase-deficient patients directly in contact with thymic epithelial monolayers induced receptors for sheep erythrocytes (E rosettes). Pretreatment of these monolayers with erythro-9-(2-hydroxy-3-nonyl) adenine, an inhibitor of adenosine deaminase, prevented differentiation only of adenosine deaminase-deficient T cell precursors, and this could be restored by providing adenosine deaminase following the incubation with thymic epithelial cells. These studies indicate a role for adenosine deaminase in the earliest stages of T cell differentiation.
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000243:Adenosine Deaminase / Q000172:deficiency / Q000502:physiology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D009700:Nucleoside Deaminases / Q000502:physiology*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000166:cytology / Q000201:enzymology*
C010192:9-(2-hydroxy-3-nonyl)adenine; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; D000225:Adenine