Formalin-treated bacteria as selective B cell mitogens in the study of lymphocytes from patients with hypogammaglobulinaemia.
Staphylococcus aureus strain Cowan I, Haemophilus influenzae, and Branhamella catarrhalis that stimulate human B lymphocytes, but not T lymphocytes, were used to study the B lymphocyte function in patients with hypogammaglobulinaemia. Lymphocytes from two patients with no circulating B cells were not stimulated to increased DNA synthesis by these bacterial mitogens. The lymphocytes of six patients with hypogammaglobulinaemia and normal numbers of circulating B cells responded to the bacterial mitogens but in five patients the response was decreased in comparison with healthy controls.
D000361:Agammaglobulinemia / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D001419:Bacteria / Q000276:immunology*; D002478:Cells, Cultured; D005557:Formaldehyde; D006193:Haemophilus influenzae / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D008934:Mitogens; D009346:Neisseriaceae / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology; D013211:Staphylococcus aureus
D007136:Immunoglobulins; D008934:Mitogens; D010835:Phytohemagglutinins; D005557:Formaldehyde