[Alpha 1-antitrypsin: production and binding of in vitro stimulated peripheral blood lymphocytes].
Three day cultures of human peripheral blood lymphocytes (PBL) contain significant numbers of lymphoblasts binding 125I-alpha 1-AT as well as those with membrane-associated alpha 1-AT detectable by immunofluorescence. Co-cultivation of PBL with T cell mitogens leads to the appearance of lymphoblasts with membrane-associated alpha 1-AT while B cell mitogens induce lymphoblasts with a binding capacity for 125I-alpha 1-AT. It could be shown that unstimulated lymphocytes neither have membrane-associated alpha 1-AT nor possess a binding capacity for it. The highest number of lymphoblasts with membrane-associated alpha 1-AT was found in Con A stimulated cultures as revealed by indirect immunofluorescence. By contrast, PHA or PPD stimulated cultures contained much less such cells of lower fluorescence intensity. Co-cultivation of PBL with the B cell mitogens LPS and rabbit-anti-human gammaglobulin-IgG-F(ab')2 induced lymphoblasts with a clear binding capacity for 125I-alpha 1-AT, while dextran-sulphate had no effect. A possible regulative role of alpha 1-AT in the immune response is discussed.
D006801:Humans; D007457:Iodine Radioisotopes / Q000378:metabolism; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008934:Mitogens / Q000494:pharmacology; D011863:Radioimmunoassay; D000515:alpha 1-Antitrypsin / Q000378:metabolism*
D007457:Iodine Radioisotopes; D008934:Mitogens; D000515:alpha 1-Antitrypsin
Alpha 1-Antitrypsin: Produktion und Bindung durch in vitro stimulierte periphere Blutlymphozyten.