Susceptibility to in vitro tolerance induction of adult B cells from mice with an X-linked B-cell defect.
Previous studies from this laboratory have indicated that the susceptibility to in vitro tolerance induction is restricted to B cells early in their development (12,14). In this study, a modification of the in vitro splenic focus technique was used to determine whether 2,4-dinitrophenyl (DNP)-specific splenic B cells from adult (CBA/N X DBA/2)F1 males are susceptible to in vitro tolerance induction. The results demonstrate that greater than 50% of the DNP-specific B cells in the adult F1 male are tolerizable and therefore immature by this criterion. Moreover, the findings define at least two subpopulation in adult CBA/N mice, one of which is tolerizable. These findings are consistent with the hypothesis that the lymphoid population in the adult CBA/N mouse is characteristic of a neonatal B-cell population.
D000818:Animals; D000831:Animals, Newborn; D001402:B-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D004136:Dinitrobenzenes / Q000276:immunology; D005260:Female; D007108:Immune Tolerance; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000276:immunology*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009154:Mutation; D014960:X Chromosome
D004136:Dinitrobenzenes