Development of an enzyme immunoassay for cryptococcal antibody.
An enzyme immunoassay (ELISA) for measurement of cryptococcal IgG antibody in human serum is described. Clinical studies indicate that the assay is a useful addition to the currently available techniques for measuring antibodies in cryptococcosis. IgG-specific antibody (titers 4 to 1,024) was detected in the serum of 78% of the cryptococcosis patients tested and in 61% of the serum from healthy individuals with positive delayed skin hypersensitivity to cryptococcin. The micro-ELISA for cryptococcal antibody is of potential value in patient management, and in epidemiological studies.
D000818:Animals; D000908:Antibodies, Fungal / Q000032:analysis*; D003453:Cryptococcosis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D011817:Rabbits
D000908:Antibodies, Fungal; D007074:Immunoglobulin G