[Characteristics of attenuated immunogenic mutants of shigella flexneri selected from phosphomycin-resistant clones].
Sh. flexneri mutants No. 2a, resistant to phosphomycin (25 microgram/ml), differ in their fermentability in respect to glucose, mannitol, mannose and fructose. The mutants fall into 7 groups by this characteristic. All the resistant mutants, irrespective of the type of hydrocarbon fermentation, have lost their ability of causing keratoconjunctivitis. The decreased virulence of the phosphomycin-resistant mutants is linked with the loss of their penetration properties, although their adhesiveness and proliferative capacity on the surface HeLa cells is retained. A change in the virulence of the mutants under study is not connected with disturbances in the synthesis of O-antigen LPS. Most of these mutants are highly immunogenic.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002999:Clone Cells; D004352:Drug Resistance, Microbial; D005578:Fosfomycin / Q000494:pharmacology*; D006168:Guinea Pigs; D006367:HeLa Cells; D009154:Mutation; D012763:Shigella flexneri / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D014611:Vaccination; D014774:Virulence
D000900:Anti-Bacterial Agents; D005578:Fosfomycin
Kharakteristika attenuirovannykh, immunogennykh mutantov shigell Flexnera, otobrannykh sredi klonov, rezistentnykh k fosfomitsinu.