title: The prosthetic group of methanol dehydrogenase. Purification and some of its properties.
abstract: Methanol dehydrogenases isolated from bacteria belonging to different classes of methylotrophs contain the same prosthetic group. A procedure for its purification from whole cells is given. The reduced and oxidized form of the enzyme from Hyphomicrobium X and those of the isolated group are compared and it is concluded that the latter indeed functions in the enzyme. Further evidence is presented that the prosthetic group is not a pterine or lumazine derivative, but a water-soluble nitrogen-containing quinone.
mesh_terms: D000429:Alcohol Oxidoreductases; D001419:Bacteria / Q000201:enzymology*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D008623:Mercaptoethanol; D000432:Methanol; D011809:Quinones / Q000302:isolation & purification*; D013053:Spectrophotometry
chemical_list: D011809:Quinones; D008623:Mercaptoethanol; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor); D000432:Methanol