title: Lipid synthesis during the Escherichia coli cell cycle.
abstract: Lipid synthesis was examined in Escherichia coli cells at different stage of cell division. Exponentially growing cells were pulse-labeled with appropriate isotopes for 0.1 generation time, inactivated, and separated by size on a sucrose gradient. An abrupt increase in the rate of lipid synthesis occurred which was coincident with the initiation of cross walls. In contrast, the rate of protein synthesis during this same interval remained constant, resulting in an increased lipid/protein ratio in dividing cells. No changes in the composition of phospholipid head groups, fatty acids, or phospholipid molecular species were observed in cells at different stages of division. The observed increase in the rate of lipid synthesis may reflect a means by which the activities of membrane-associated enzymes are modulated during cross wall formation.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis; D002453:Cell Cycle; D002473:Cell Wall / Q000378:metabolism; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D007700:Kinetics; D008055:Lipids / Q000032:analysis / Q000096:biosynthesis*; D010743:Phospholipids / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D008055:Lipids; D010743:Phospholipids