title: An inexpensive, portable, battery-operated photometer for the reading of ELISA tests in microtitration plates.
abstract: We described an inexpensive, portable, battery-operated photometer which can read the enzyme-linked immunosorbent assay in 96-well microtitre trays. An example is given of its use to measure IgG antibody to human collagen type II in a serum sample from a rheumatoid arthritis patient known to have a high titre of antibody to cartilage by immunofluorescence. When the same samples were read first with the portable photometer and then in a spectrophotometer (Unicam SP 1800), the accuracy of the two devices was comparable, but the readings were obtained far more quickly in the photometer.
mesh_terms: D000293:Adolescent; D003094:Collagen; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D008297:Male; D010783:Photometry / Q000295:instrumentation*; D013053:Spectrophotometry
chemical_list: D003094:Collagen