Quantification of arabinitol in serum by selected ion monitoring as a diagnostic technique in invasive candidiasis.
D-Arabinitol was identified by mass spectrometry as a metabolite of Candida albicans and Candida tropicalis. For quantification, serum was deproteinized with acetone, the supernatant was evaporated to dryness, the silyl derivative was formed, and a portion was injected into a combined gas chromatograph-mass spectrometer system. Erythritol or 2-deoxy-galactitol was the internal standard. The protonated molecular ions, obtained in chemical ionization with isobutane, were monitored. For 39 normal subjects the mean endogenous arabinitol concentration was 0.52 microgram/ml (standard deviation, +/- 0.34). An increment of 0.2 microgram of arabinitol per ml could be quantified. Of 11 cases with diagnosed invasive candidiasis, 9 had arabinitol levels > 1.2 microgram/ml (8 microM) in the range of 1.2 to 25.0 micrograms/ml; the remaining 2 cases had levels in the normal range. Six cases of diagnosed colonized candidiasis showed normal arabinitol levels.
D000293:Adolescent; D000328:Adult; D001089:Arabinose / Q000031:analogs & derivatives / Q000097:blood; D002175:Candida / Q000378:metabolism; D002176:Candida albicans / Q000378:metabolism; D002177:Candidiasis / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D007477:Ions; D013058:Mass Spectrometry; D008875:Middle Aged; D013402:Sugar Alcohols / Q000097:blood*
D007477:Ions; D013402:Sugar Alcohols; D001089:Arabinose; C014999:arabitol