Transfer of plasmid pRD1 from Escherichia coli to Azospirillum brasilense.
Data are presented which indicate that plasmid pRD1 can be transferred from Escherichia coli to strains of Azospirillum brasilense with a frequency of about 10(-7). The reverse was also possible; in this case the frequency of transfer appeared to be much higher, about 5 x 10(-1). Transfer of the plasmid was also obtained between strains of A. brasilense; in this cross the transfer frequency was very high (about 10(-1)). Moreover the pRD1 plasmid seems very stable in A. brasilense cells.
D001395:Azotobacter / Q000235:genetics; D003227:Conjugation, Genetic; D003433:Crosses, Genetic; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D011548:Pseudomonadaceae / Q000235:genetics*; D011995:Recombination, Genetic