[A study on the immunity of pregnancy. The localization of HCG on the cell membrane of lymphocytes (author's transl)].
The study was made for women in 10 weeks and 12 weeks of pregnancy, and also for non-pregnant women and men as controls. Heparinized peripheral venous blood of them were obtained, and lymphocytes were separated by specific gravity centrifugation by Lymphoprep, to be used as samples. For enzyme reaction, at first a separated lymphocyte PBS suspension was smeared on a glass sheet, dried in air and fixed for 15 minutes by 4% PLP solution, then to make antigen-antibody reaction by direct method. After the reaction, the reaction product was fixed for 10 minutes by 2% glutaraldehyde and, with color developed by Karnovsky method. Antibodies used were (1) HRP-anti human alpha-HCG rabbit Fab', (2) HRP-anti human beta-HCG rabbit Fab' and (3) HRP-anti human native HCG rabbit Fab', and furthermore, (4) HRP-N rabbit serum and (5) HRP-anti rabbit IgG goat F(ab)2 were used as staining controls. In the reaction group of pregnant cases with alpha-HCG, beta-HCG and N-HCG antisera, the brown color of reaction products was observed on lymphocyte cell membranes to prove the localization of HCG. The existence of lymphocytes negative in the reaction and the difference in color development degrees among lymphocytes positive in the reaction were also observed, to show quantitative difference in localization. In the group of the controls and in the staining control group of pregnant cases, no reaction was observed at all.
D002462:Cell Membrane / Q000276:immunology; D006063:Chorionic Gonadotropin / Q000097:blood* / Q000276:immunology; D005260:Female; D006801:Humans; D007109:Immunity; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D011247:Pregnancy
D006063:Chorionic Gonadotropin