[Fluorescent method of determining the pH in cells that uses the "fluorochromasia" phenomenon].
A new fluorescence method for pH determination inside the cells using the phenomenon of "fluorochromasia" has been developed, that makes it possible to measure intracellular pH changes in both a single cell *by means of cytofluorimeter) and in a population of cells (by means of fluorimeter) in the pH range below 7.4 within the accuracy of 0.1 unit of pH. It is most advisable to use this method for studying effects of different agents inducing fast shifts in the intracellular pH.
D000818:Animals; D002478:Cells, Cultured; D002999:Clone Cells / Q000166:cytology; D006224:Cricetinae; D003412:Cricetulus; D003584:Cytological Techniques; D005452:Fluoresceins / Q000494:pharmacology; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration
D005452:Fluoresceins
Fluorestsentnyĭ metod opredeleniia pH v kletkakh, ispol'zuiushchiĭ iavlenie "fluorokhromazii".