The concentrations of cephalosporins in tissue fluid of rats with localized infection.
Rats implanted with multiperforated subcutaneous plastic capsules were used as a model for study of the penetration of four cephalosporins into sites infected with Escherichia coli. The concentrations of the antibiotics were determined in uninfected and infected cages microbiologically up to 4 h after treatment. All four cephalosporins entered infected capsules faster than the control ones and the peak concentrations were attained 30-60 min earlier. The clearance of the antibiotics from infected capsules was faster than from uninfected ones, probably due to inactivation by microbial beta-lactamase(s). Serum protein binding may account for differences in the penetration rate of cephalosporins, but serum level and serum half-time seem to contribute to their kinetic profile in tissue fluid.
D000818:Animals; D002435:Cefamandole / Q000378:metabolism; D002437:Cefazolin / Q000378:metabolism; D002509:Cephaloridine / Q000378:metabolism; D002511:Cephalosporins / Q000378:metabolism*; D002515:Cephradine / Q000378:metabolism; D004195:Disease Models, Animal; D004927:Escherichia coli Infections / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051381:Rats; D001618:beta-Lactamases / Q000494:pharmacology
D002511:Cephalosporins; D002435:Cefamandole; D001618:beta-Lactamases; D002515:Cephradine; D002437:Cefazolin; D002509:Cephaloridine