Effect of endotoxin on oxygen consumption by a flow-controlled canine hind-limb preparation.
The effect of endotoxin upon oxygen consumption in a peripheral vascular bed was assessed in a canine hind-limb preparation. The left iliac vessels in 12 adult male mongrel dogs were isolated, the artery cannulated proximally and distally such that arterial inflow could be routed through a roller pump, and a nonocclusive catheter placed in the vein. Oxygen consumption was then calculated as the product of flow rate and and arteriovenous oxygen content difference at a series of flow rates before and after the systemic administration of endotoxin (3 mg/kg) or saline solution. At each flow rate, oxygen consumption by the extremities of endotoxic animals was significantly greater (P < 0.01) than that measured in control animals. This finding mitigates the hypothesis that endotoxin has a direct effect on cells reducing tissue oxygen consumption.
D000818:Animals; D001794:Blood Pressure; D001831:Body Temperature; D004285:Dogs; D004731:Endotoxins / Q000494:pharmacology*; D004926:Escherichia coli; D006614:Hindlimb / Q000098:blood supply / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008297:Male; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption / Q000187:drug effects*; D012039:Regional Blood Flow; D014655:Vascular Resistance
D004731:Endotoxins; D010100:Oxygen