[Serological diagnosis of human brucellosis by means of an immunoenzymatic technic (ELISA). I.--Estimate of some parameters of the reaction and practical application (author's transl)].
The authors propose a new immunoenzymatic method (ELISA) for human brucellosis diagnosis. They study in a logical way some of the parameters of the reaction realizable by macro- or microassay. The all method requires less than six hours. They use OMS recommended brucella suspension as antigen. The conjugate enzyme is peroxydase and human anti-immunoglobulins are either complete or specific IgM. The revelating substrate is obtained by association of hydrogen peroxyde with 5 amino-salicylic acid which gives the best colorimetric stability. The reading is made at 450 nm : automatically by micro-assay. Using only one sample dilution, serum quantification may be hazardous.
D002006:Brucellosis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods
Application de la technique immunoenzymatique ELISA (enzyme-linked-immunosorbent-assay) au diagnostic sérologique des brucelloses humaines. I.--Evaluation de quelques paramètres de la réaction et réalisation pratique.