Antibodies to liver-specific auto- and alloantigens after alloimmunization with liver tissue in the rat.
LEW anti-DA liver homogenate and anti-DA liver vascularized graft sera were studied using 125I anti-immunoglobulin binding assays with liver homogenate as the target tissue. In both cases, a major component of the antibodies generated was autoantibody directed at a liver-specific antigen localized intracellularly in hepatocytes. These autoantibodies could not be detected by binding or cytotoxicity assays on lymphocyte targets. In addition, a component of the antibodies was shown to be directed against a liver-specific alloantigen located on the external and intracellular membranes of hepatocytes, and coded for by a locus not linked to the major histocompatibility complex (MHC). Alloantibodies were also demonstrated to both SD-type (RT.1A) and Ia-type (RT.1B) antigens of the MHC, with a preponderance of anti-SD antibodies.
D000818:Animals; D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis*; D001324:Autoantigens / Q000032:analysis; D003433:Crosses, Genetic; D007114:Immunization; D007518:Isoantibodies / Q000032:analysis; D007519:Isoantigens / Q000032:analysis / Q000276:immunology*; D008099:Liver / Q000276:immunology*; D016031:Liver Transplantation; D008285:Major Histocompatibility Complex; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D014181:Transplantation Immunology
D001323:Autoantibodies; D001324:Autoantigens; D007518:Isoantibodies; D007519:Isoantigens