Synthesis of a chicken ovalbumin-like protein in the yeast Saccharomyces cerevisiae.
A cDNA sequence coding for chicken ovalbumin was fused to the beginning of the Escherichia coli lactose operon and recombined in vitro with a composite vector plasmid which can be propagated in both E. coli and Saccharomyces cerevisiae. Such plasmids direct the synthesis of an ovalbumin-like protein (OLP) in both microorganisms, probably because the E. coli lac regulatory region has some promoter activity in yeast. The yeast strains produce about 1 000 to 5 000 molecules of ovalbumin-like protein per cell.
D000818:Animals; D002645:Chickens / Q000235:genetics*; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D004274:DNA, Recombinant / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D010047:Ovalbumin / Q000235:genetics*; D010957:Plasmids; D014176:Protein Biosynthesis; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004269:DNA, Bacterial; D004271:DNA, Fungal; D004274:DNA, Recombinant; D010047:Ovalbumin; D004247:DNA