Automated immunofluorescence test for toxoplasmosis.
A soluble-antigen fluorescent-antibody technique for serodiagnosis of toxoplasmosis, employing a special fluorometer (FIAX; International Diagnostic Technology, Santa Clara, Calif.), was evaluated in comparison with a standard indirect fluorescent-antibody method which uses a fluorescence microscope. Titers obtained by the new method on 324 serum samples correlated very well with those of the conventional procedure. There was good intralaboratory and interlaboratory agreement in runs on selected samples. The FIAX method is simple, rapid, and reliable and has the advantage of objectivity.
D000906:Antibodies / Q000032:analysis; D001322:Autoanalysis; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006801:Humans; D008856:Microscopy, Fluorescence; D012698:Serologic Tests; D013050:Spectrometry, Fluorescence; D014122:Toxoplasma / Q000276:immunology; D014123:Toxoplasmosis / Q000175:diagnosis*
D000906:Antibodies