Intestinal alkaline phosphatase: an immunoprecipitation method for the determination in feces.
An immunoprecipitation method for the determination of human intestinal alkaline phosphatase (I-AP) in feces is described using anti-human I-AP IgG. Antibodies to human I-AP are monospecific after absorption with human placental alkaline phosphatase and show no cross-reactions with human liver alkaline phosphatase or alkaline phosphatase from E. coli. Normal controls demonstrate a logarithmic normal distribution of I-AP activity in feces. The standard deviation of the intra- as well as of the inter-assay variation is 11.7%. This method represents a simple quantitative non-invasive in vivo assay for brush border damage under experimental and/or clinical conditions.
D000469:Alkaline Phosphatase / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D003429:Cross Reactions; D004926:Escherichia coli / Q000201:enzymology; D005243:Feces / Q000201:enzymology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007158:Immunologic Techniques; D007422:Intestines / Q000201:enzymology*; D008099:Liver / Q000201:enzymology; D051381:Rats
D000469:Alkaline Phosphatase