Evaluation of ELISA in the diagnosis of Yersinia enterocolitica diarrhoea in children.
In a series of acute gastroenteritis in children, including 2 bacteriologically confirmed cases of Yersinia enterocolitica infection, paired sera from 286 patients were studied for the presence of Y. enterocolitica 0:3 and 0:9 antibodies, using an enzyme-linked immunosorbent assay (ELISA). In the 2 cases with positive stool culture of Y. enterocolitica 0:3 significant rise was seen in each IgM, IgG and IgA class Y. enterocolitica 0:3 antibodies. There were 6 further cases with elevated anti-Yersinia IgM: these possibly false-positive reactions were distinguished from the confirmed cases of Y. enterocolitica infection by the absence of IgG and IgA antibodies.
D000208:Acute Disease; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000175:diagnosis* / Q000382:microbiology; D003968:Diarrhea, Infantile / Q000175:diagnosis / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007223:Infant; D015009:Yersinia Infections / Q000175:diagnosis*
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M