Physiological basis for meso-tartrate sensitivity in some strains of Salmonella typhimurium.
meso-Tartrate inhibited the growth of non-meso-tartrate-utilizing strains of Salmonella typhimurium in peptone water media and mineral salts media with some, but not all, carbon sources. C-R intermediates of the tricarboxylic acid cycle or compounds readily converted to them and substrates metabolized independently of the C-6 part of the cycle spared bacteria from the inhibitory effects of meso-tartrate when added to cultures along with meso-tartrate. Experiments with cell-free extracts of non-meso-tartrate-utilizing strains from batch and continuous cultures showed that meso-tartrate was a competitive inhibitor of isocitrate dehydrogenase and isocitrate lyase activities and also inhibited citrate synthase and malate synthase activities. The synthesis of these enzymes was not inhibited by meso-tartrate. The isocitrate enzymes of meso-tartrate-utilizing strains of S. typhimurium were similarly inhibited by meso-tartrate, but inhibition of the growth of meso-tartrate-utilizing strains was demonstrable only in uninduced cultures in which the intracellular concentrations of meso-tartrate were high.
D002950:Citrate (si)-Synthase / Q000037:antagonists & inhibitors; D003470:Culture Media; D007521:Isocitrate Dehydrogenase / Q000037:antagonists & inhibitors; D007522:Isocitrate Lyase / Q000037:antagonists & inhibitors; D008292:Malate Synthase / Q000037:antagonists & inhibitors; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013644:Tartrates / Q000378:metabolism / Q000494:pharmacology*
D003470:Culture Media; D013644:Tartrates; D007521:Isocitrate Dehydrogenase; D002950:Citrate (si)-Synthase; D008292:Malate Synthase; D007522:Isocitrate Lyase