title: Flow-cytometric analysis of human basophil degranulation. III. Degranulation induced by allergens and antibodies in hay fever and bee venom allergic patients.
abstract: Human basophil degranulation responses to grass pollen and whole bee venom as allergens and to anti-IgE and anti-IgG4 as antibodies in hay fever and bee venom allergic patients were assessed using a flow-cytometric system. The concordance of basophil degranulation with skin tests and RAST assays reached more than 90%. These allergens and antibodies could degranulate basophils in a dose-dependent manner; however, there was a wide variation in the response of basophils obtained from different individuals. Moreover, a significant correlation was observed between the degree of degranulation by allergens and anti-IgE, or by allergens and anti-IgG4, but this was not the case between serum IgE concentrations and basophil response to anti-IgE. These data suggest that 'releasability' of basophils upon challenge by allergens and antibodies is also an important parameter in determining the degree of hypersensitivity in each subject.
mesh_terms: D000485:Allergens; D000818:Animals; D000906:Antibodies; D001491:Basophils / Q000276:immunology*; D001514:Bee Venoms / Q000276:immunology; D003594:Cytoplasmic Granules / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D006041:Goats; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007158:Immunologic Techniques; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology
chemical_list: D000485:Allergens; D000906:Antibodies; D001514:Bee Venoms; D007074:Immunoglobulin G; D007073:Immunoglobulin E