Development of a multivalent live vaccine active against a wide range of Enterobacteriaceae.
We have constructed a deletion  mutant of E. coli which lacks O-antigen - "deep rough". Living bacteria of this strain were injected repeatedly in high numbers into mice and chicks and in all cases were found to be completely harmless. In C3HeB mice, protection was obtained against a wide variety of enteric bacteria and was accompanied by an appreciable increase in titer of antibodies which cross react with LPS extracted from these bacteria. Preliminary experiments indicate that the vaccine provides protection against avian coli pathogens.
D000818:Animals; D001424:Bacterial Infections / Q000517:prevention & control* / Q000662:veterinary; D001428:Bacterial Vaccines / Q000592:standards / Q000627:therapeutic use*; D002645:Chickens; D002872:Chromosome Deletion; D004755:Enterobacteriaceae / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D018805:Sepsis / Q000517:prevention & control
D001428:Bacterial Vaccines