[Pathobiology of anoxic cells].
Some experimental models for the study of the metabolism of anoxic cells are briefly outlined and the meaning and limits of the results obtained with different biochemical techniques are critically evaluated. Metabolic changes occurring in the mitochondria of anoxic cells are discussed in relation to the decreased production of ATP and to the shift towards a more reduced state of both the mitochondria and the cytosol. The activation of the microsomal phospholipase, and the concurrent impairment of the microsomal Ca2+-pump, seem to play a key role in the pathogenesis of anoxic injury. The membranes of the endoplasmic reticulum, the polysomes and the soluble factors of protein synthesis are also damaged in anoxic cells. The degree of impairment of the machinery for protein synthesis affects the capacity of the cell to repair the many kinds of damages caused by anoxia, and to overcome the critical point represented by the re-establishment of the normal oxygen supply following the period of oxygen deprivation.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002477:Cells / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D000860:Hypoxia / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism
D000255:Adenosine Triphosphate; D002118:Calcium
Patobiologia della cellula anossica.