A radiometric assay for the combined measurement of phagocytosis and intracellular killing of Candida albicans.
A radiometric assay for combined measurement of phagocytosis and intracellular killing of Candida albicans by human polymorphonuclear leucocytes (PMNs) is presented. The assay, based upon the incorporation of 3H-uridine into the micro-organisms, makes it possible to measure phagocytosis and intracellular killing simultaneously but independently in a single sample. Thus it is possible to determine in a single assay whether increased survival of the micro-organism is due to reduced ingestion or reduced ability of the PMN to kill. The assay is objective, quantitative and convenient for clinical application. It is also suitable for analysing the effects of various agents, including serum factors and drugs, on PMN function.
D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002176:Candida albicans; D003176:Complement C3 / Q000276:immunology; D006358:Hot Temperature; D006801:Humans; D008722:Methods; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects; D013997:Time Factors; D014316:Tritium
D000900:Anti-Bacterial Agents; D003176:Complement C3; D014316:Tritium