Enzyme immunoassay for staphylococcal enterotoxin A.
An enzyme immunoassay procedure specifying alkaline phosphatase-labeled enterotoxin A was used to determined enterotoxin in standardized solutions and food extracts. Staphylococcus aureus Cowan 1 cells were used to separate unbound from antibody-bound enterotoxin. The method is sensitive to 2 ng enterotoxin A/mL; cross reactions with other staphylococcal enterotoxins did not interfere with the specificity. The method is sensitive and precise enough to serve as a reliable alternative to radioimmunoassay for enterotoxin A.
D000818:Animals; D002417:Cattle; D004768:Enterotoxins / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D007124:Immunoenzyme Techniques
D004768:Enterotoxins; C026066:enterotoxin A, Staphylococcal