Kinetics of benzo[a]pyrene induced mutagenesis in a highly sensitive salmonella/microsome assay.
The kinetics of benzo[a]pyrene induced mutagenesis in Salmonella typhimurium TA98 and TA100 were studied using a liquid-phase assay and were compared to kinetics of metabolism of benzo[a]pyrene in the same system. Mutagenesis was terminated by the addition of pyrene + menadione at selected times. Under conditions of the assay using hepatic microsomes from 3-methyl-cholanthrene pretreated rats, metabolism of benzo[a]pyrene was over 50% complete in 5 min, and metabolism of initial metabolites became appreciable after this time. The revertant frequency (revertants/10(8) survivors) and the production of tertiary metabolites increased sharply after 5 min and leveled off at 40 min.
D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism / Q000494:pharmacology*; D001711:Biotransformation; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D007700:Kinetics; D008862:Microsomes, Liver / Q000378:metabolism; D009153:Mutagens; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects
D001580:Benzopyrenes; D009153:Mutagens