Cadmium-113 nuclear magnetic resonance studies of bovine insulin: two-zinc insulin hexamer specifically binds calcium.
By use of cadmium-113 nuclear magnetic resonance spectroscopy, a specific calcium ion binding site has been identified in the bovine two-zinc insulin hexamer. This site is composed of six glutamyl carboxylate groups clustered in the center of the hexamer, and is distinct from the normal zinc ion binding sites.
D000818:Animals; D001665:Binding Sites; D002104:Cadmium; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D007328:Insulin / Q000378:metabolism; D007554:Isotopes; D008024:Ligands; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D011487:Protein Conformation; D015032:Zinc / Q000378:metabolism*
D007328:Insulin; D007554:Isotopes; D008024:Ligands; D046911:Macromolecular Substances; D002104:Cadmium; D015032:Zinc; D002118:Calcium