[Heparin complexes with DNA: formation, properties and methylation in vitro].
Heparin complexes with DNA were obtained in vitro, the components being taken in weight ratios of 1 : 6 or 1 : 12, respectively. The complexes obtained exhibited the anticoagulating activity and antipolymerizing properties in the presence of a fibrin-monomer and an ability to dissolve nonstabilized fibrin. An intravenous injection of these complexes to rats increased the total and non-enzymatic fibrinolytic activity of blood plasma and its anticoagulating properties, while an intravenous injection of DNA caused only a short-term hypercoagulating effect. The DNA modified by heparin altered its acceptor abilities in an in vitro methylation reaction in the presence of DNA-methylases from animal and bacterial cells. Incorporation of methyl groups into DNA showed a decrease, apparently due to heparin blocking of some methylable sequences. An addition of heparin to a system containing purified DNA, DNA-methylase and S-adenosylmethionine was accompanied by a concentration-dependent inhibition of the methyl group incorporation into DNA.
D000818:Animals; D004247:DNA; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism; D004926:Escherichia coli; D005337:Fibrin / Q000502:physiology; D005342:Fibrinolysis / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology; D007700:Kinetics; D008745:Methylation; D051381:Rats; D012436:S-Adenosylmethionine
D012436:S-Adenosylmethionine; D005337:Fibrin; D006493:Heparin; D004247:DNA; D004248:DNA (Cytosine-5-)-Methyltransferases
Poluchenie, svoĭstva i metilirovanie in vitro kompleksov geparina s DNK.