Preparation of zymosan from yeast cell walls.
Cell walls of the yeast Saccharomyces cerevisiae after disintegration and protoplasm removal by centrifugation and repeated washing were suspended in 0.5 M Na2HPO4, pH 7.8-8.0, as a 5% or 10% suspension, depending on the mode of heating. The suspension was boiled for 3 h, purified by repeated washing with water and ethanol and dried. The yield was approximately 1.8% of the starting amount of pressed commercial baker's yeast.
D002473:Cell Wall / Q000032:analysis; D008722:Methods; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D015054:Zymosan / Q000032:analysis / Q000302:isolation & purification*
D015054:Zymosan