A method for culturing human hair follicle cells.
For the first time a method for culturing human hair follicle cells is described. The bovine eye lens capsule, a basement membrane-like structure, is used as the substrate for the cultures. In a culture medium supplemented with hydrocortisone and insulin about 70% of the original follicles will form growing colonies of diploid keratinocytes.
D002452:Cell Count; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000295:instrumentation; D006197:Hair / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology
D007328:Insulin; D006854:Hydrocortisone