Isolation and mapping of a mutation in Escherichia coli with altered levels of ribonuclease H.
A mutant of Escherichia coli with altered levels of ribonuclease (RNase) H was isolated after mutagenesis with ethyl methane sulfonate. A procedure for assaying RNase H in partially purified extracts was used to screen approximately 1,500 colonies for variations in RNase H activity. Confirmation of a lower level of RNase H in the mutant was accomplished by analysis of RNase H in sodium dodecyl sulfate-polyacrylamide gels. By Hfr, F', and P1 transduction mapping, the genetic locus responsible for the lower levels of RNase H was located at 5.1 min on the E. coli chromosome. This mutation (rnh) represents a new locus on the E. coli chromosome. The only phenotypic characteristic of this mutation which has been observed to date is the lower level of RNase H (30% of parental values).
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004261:DNA Replication; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D009154:Mutation; D010957:Plasmids; D012260:Ribonucleases / Q000235:genetics*
D012260:Ribonucleases