title: Solubilization and reconstitution of the lactose transport system from Escherichia coli.
abstract: The lactose transport system from Escherichia coli was solubilized with octylglucoside and reconstituted into liposomes by an octylglucoside dilution procedure. The reconstituted proteoliposomes exhibited lactose counterflow and membrane potential-driven lactose transport.
mesh_terms: D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D007700:Kinetics; D007785:Lactose / Q000378:metabolism*; D008081:Liposomes; D008563:Membrane Lipids / Q000302:isolation & purification / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins; D012995:Solubility; D027981:Symporters
chemical_list: D029968:Escherichia coli Proteins; C050221:LacY protein, E coli; D008081:Liposomes; D008563:Membrane Lipids; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D027981:Symporters; C021499:lactose permease; D007785:Lactose