The occurrence of two ribosomal ribonucleases depending on growth phase in yeast. Induction of ribonuclease in glucose-starved cells.
There are two latent ribonucleases associated with the 40 S subunits of yeast ribosomes which differ in their digestion products, pH optimum, molecular weight, and in their activity during growth phase. The 3'-nucleotide-producing enzyme is active only in the late logarithmic or stationary growth phase, whereas the ribonuclease which produces 5'-nucleotides is present at all growth phases. The enzymes were separated by affinity chromatography and were partially characterized. By changing growth conditions--i.e. decreasing and increasing the glucose concentration in the medium--the activity of the 3'-ribonuclease could be induced or reduced.
D002453:Cell Cycle; D002846:Chromatography, Affinity; D004790:Enzyme Induction; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D012260:Ribonucleases / Q000096:biosynthesis* / Q000302:isolation & purification; D012270:Ribosomes / Q000201:enzymology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D012260:Ribonucleases; D005947:Glucose