Alloimmune neonatal thrombocytopenia: platelet antibody detection using the indirect immunofluorescence test (IIFT).
Two cases of neonatal alloimmune thrombocytopenia are presented. Using the indirect immunofluorescence test on platelets in suspension, the presence of non-agglutinating platelet specific antibodies with the specificity anti-Zwa could be demonstrated. In both cases diagnosis of alloimmunization was made before delivery of a second child, typing of the family was done and recommendations could be made concerning clinical management. Compatibility testing between the mother's serum and donor platelets (Zwa-negative) was predictable of a normal transfused platelet survival in the newborn.
D000906:Antibodies / Q000302:isolation & purification*; D001792:Blood Platelets / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000276:immunology*; D011247:Pregnancy; D011296:Prenatal Diagnosis; D013921:Thrombocytopenia / Q000175:diagnosis / Q000276:immunology*
D000906:Antibodies