The selection of amber mutations in genes required for completion of start, the controlling event of the cell division cycle of S. cerevisiae.
Using a modification of a procedure developed for the isolation of temperature-sensitive mutants defective in the start event of cell division, amber mutations were obtained for two Class-I start genes, cdc28 and cdc37. Genetic analysis demonstrated that co-segregation of an amber suppressor with such alleles was required for viability of spores subsequent to meiosis. These mutations are expected to be useful in the identification of the molecular products of the genes cdc28 and cdc37.
D000483:Alleles; D002455:Cell Division; D003227:Conjugation, Genetic; D005796:Genes; D005815:Genetic Code; D009154:Mutation; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000302:isolation & purification