Incorporation and metabolism of 2-acyl lysophospholipids by Escherichia coli.
The incorporation of 2-acyl lysophospholipids into Escherichia coli, and their metabolism were studied. 2-[14C]Acyl lysophosphatidylethanolamine could penetrate into E. coli cells and was mainly incorporated into phosphatidylethanolamine. 2-Acyl lysophosphatidylethanolamine was partially degraded, but some of it was incorporated into membrane phospholipids by acylation. 2-Acyl lysophosphatidylcholine also entered cells and was acylated to phosphatidylcholine. The acylation of 2-acyl lysophospholipid by the envelope fraction was also studied. Fatty acids were incorporated into 2-acyl lysophospholipids by the envelope fraction in the presence of ATP and Mg2+, and the incorporation was stimulated by acyl carrier protein, but not by coenzyme A. No acylation was observed with acyl coenzyme A as acyl donor. The acylation activities of the inner and outer membranes were examined. Pathways for degradation and modification of membrane phospholipids in E. coli are proposed.
D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D008244:Lysophosphatidylcholines / Q000378:metabolism*; D008246:Lysophospholipids; D008563:Membrane Lipids / Q000378:metabolism*; D010169:Palmitic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000096:biosynthesis; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism
D002250:Carbon Radioisotopes; D008244:Lysophosphatidylcholines; D008246:Lysophospholipids; D008563:Membrane Lipids; D010169:Palmitic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; C008301:lysophosphatidylethanolamine