Activity determination of 3-iodopyridineadenine dinucleotide and its phosphate as hydride acceptors in the presence of dehydrogenases using a coupled redox system.
A new procedure for the activity measurement of NAD(P)+-dependent dehydrogenases has been devised using an electron-transferring agent, phenazine methosulfate, and an electron acceptor, 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide. The reduction of the latter is determined by an increase in absorbance at 578 nm. 3-Iodopyridineadenine dinucleotide was found to be active as an hydride acceptor with horse liver alcohol dehydrogenase and lactate dehydrogenase but showed no activity with glyceraldehyde-3-phosphate dehydrogenase nor did its phosphate with 3-phosphogluconate dehydrogenase.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D004284:Dogfish; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D006736:Horses; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D009243:NAD / Q000031:analogs & derivatives* / Q000378:metabolism; D009249:NADP / Q000031:analogs & derivatives* / Q000378:metabolism; D010084:Oxidation-Reduction; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism*; D011817:Rabbits; D012756:Sheep; D013379:Substrate Specificity
D009243:NAD; D009249:NADP; C018983:3-iodopyridine-adenine dinucleotide; C018984:3-iodopyridine-adenine dinucleotide phosphate; D000429:Alcohol Oxidoreductases; D007770:L-Lactate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases