Detection of immune deposits in glomeruli. Light-, fluorescence- and electron microscopic investigation of consecutive sections.
Methods which permit a close comparative light-, fluorescence- and electron microscopical investigation of kidney tissue are lacking. In this study is described a technique for handling of formalin sections, 40 micrometer-160 micrometer thick in preparatory steps which include proteolytic treatment for "unmasking" of immune deposits and which end in Epon embedded, consecutive semithin and ultrathin sections. These are shown to be able to use for detailed immuno-morphological comparison.
D000818:Animals; D005260:Female; D006652:Histological Techniques; D007136:Immunoglobulins / Q000032:analysis*; D007678:Kidney Glomerulus / Q000276:immunology*; D008722:Methods; D051379:Mice; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence
D007136:Immunoglobulins