Lysis and protoplast formation of group B streptococci by mutanolysin.
Group B streptococci, refractory to previously tested muralysins under physiological conditions, were successfully converted to protoplasts by use of a recently describede N-acetyl muramidase, mutanolysin, derived from a streptomycete. Purified enzyme was effective, but crude preparations, although degrading cell walls, simultaneously produced peculiar effects of cytoplasmic coagulation, retention of cell shape, loss of some intracellular enzymes, and a rise in optical density. Addition of purified mutanolysin to the array of muralysins (group C streptococcal phage-associated lysin, lysozyme), previously successful in preparing protoplasts of different streptococci, now makes possible enzymatic preparation of protoplasts of streptococci of groups A, B, C. D. G, and H.
D001433:Bacteriolysis; D002473:Cell Wall / Q000187:drug effects; D010450:Endopeptidases / Q000494:pharmacology*; D010457:Peptidoglycan / Q000494:pharmacology; D011523:Protoplasts / Q000648:ultrastructure*; D013292:Streptococcus agalactiae / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure
D010457:Peptidoglycan; D010450:Endopeptidases; C021816:mutanolysin