[Cultivation of monolayer cultures of primary and transplantable animal cells in a device with regulated growth conditions].
Primary, diploid and continuous cell lines were cultivated in the apparatus "Cellotron-1". Rashig's ring was used as an underlay in the apparatus. Fresh medium perfusion, pH control, the medium aeration, temperature control, as well as dissolved oxygen control were exercised in the cultivator. Various cell types have demonstrated a significant increase as compared to the control stationary cultivation: diploid green monkey embryo cells increased 7.5-fold for 5 days, continuous green monkey kidney cells 8-fold for 4 days, primary monkey kidney cells 2-fold for 7 days.
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002478:Cells, Cultured; D002522:Chlorocebus aethiops / Q000196:embryology; D003584:Cytological Techniques / Q000295:instrumentation*; D004171:Diploidy; D007185:Incubators / Q000295:instrumentation*; D007668:Kidney
Kul'tivirovanie odnosloĭnykh kul'tur pervichnykh i perevivaemykh kletok zhivotnykh v apparate s regulirovaniem usloviĭ rosta.