Solubilization and reconstitution of the lactose transport system from Escherichia coli.
The lactose transport system from Escherichia coli was solubilized with octylglucoside and reconstituted into liposomes by an octylglucoside dilution procedure. The reconstituted proteoliposomes exhibited lactose counterflow and membrane potential-driven lactose transport.
D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D007700:Kinetics; D007785:Lactose / Q000378:metabolism*; D008081:Liposomes; D008563:Membrane Lipids / Q000302:isolation & purification / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins; D012995:Solubility; D027981:Symporters
D029968:Escherichia coli Proteins; C050221:LacY protein, E coli; D008081:Liposomes; D008563:Membrane Lipids; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D027981:Symporters; C021499:lactose permease; D007785:Lactose