Purification and characterization of erythrogenic toxins. II. Communication: in vivo biological activities of erythrogenic toxin produced by streptococcus pyogenes strain ny-5.
Some in vivo biological activities of a purified erythrogenic toxin preparation from strain NY-5 of group A streptococci (ET NY-5) were studied. After intracutaneous administration in a dose of minimally 5 X 10(-8) mg, the toxin provoked a skin reaction in old guinea pigs. No skin reaction could be elicited in young adult rabbits. ET NY-5 was pyrogenic for rabbits; the MPD-4 amounted to 0.017 microgram/kg intravenously. The pyretic response had a delayed onset and then rose gradually up to hour 5 postinjection. Intravenous administration led to changes in circulating leukocyte counts, viz. brisk acute granulocytopenia and protracted lymphopenia. The method of pyrogenic cross tolerance revealed that ET NY-5 contains two types of pyrogenic exotoxin, A and C.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D001812:Blood-Brain Barrier / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D004890:Erythema / Q000139:chemically induced; D006168:Guinea Pigs; D007958:Leukocyte Count; D008934:Mitogens; D011749:Pyrogens; D011817:Rabbits; D012882:Skin Tests; D013297:Streptococcus pyogenes / Q000276:immunology*; D014118:Toxins, Biological / Q000302:isolation & purification*
D008934:Mitogens; D011749:Pyrogens; D014118:Toxins, Biological