[Production of a macrophage migration suppression factor when T-cells react with mutant H-2 antigens in mixed lymphocyte cultures].
The ability of T-cells producing the migration inhibition factor (MIF) to recognize point mutations of the K-end of the H-2 complex was revealed in a primary mixed lymphocyte culture. Nonimmune C57BL/6Y (H-2bm) lymphocytes produce MIF in response to the H-2 antigens of B6..C-G (zI)/Y (H-2bm1) and B6.M505/Y (H-2bm3) mutant strains. Similarly lymphocytes of the mutant strains react to the H-2b wild-type and reciprocally to each other. T-cell dependence of these reactions was shown by their abolition after pretreatment of responder cells with Thy-1.2 antiserum in the presence of complement. The ability of MIF-producing cells to recognize serologically unrecognizable H-2bm1 and H-2bm3 mutant antigens demonstrates high immunologic specificity and sensitivity of the MIF-reaction.
D000818:Animals; D002478:Cells, Cultured; D006183:H-2 Antigens / Q000235:genetics* / Q000276:immunology; D008214:Lymphocytes / Q000378:metabolism*; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D013601:T-Lymphocytes / Q000378:metabolism
D006183:H-2 Antigens; D008263:Macrophage Migration-Inhibitory Factors
Produktsiia faktora podavleniia migratsii makrofagov pri reaktsii T-kletok na mutantnye antigeny H-2 v smeshannoĭ kul'ture limfotsitov.