[Nature of lactate secretion by E. coli cells].
The fluxes of H+, K+ and Lac-- ions in suspension of E. coli were studied by means of ion-selective electrodes. It was shown that the stoichiometry of DCCD-sensitive ionic exchange is 2H : K+ : Lac--. It was supposed that the bacteria maintains intracellular macroscopic electroneutrality by secretion of Lac-- anions during activity of proton-potassium pump, which exchange 2H+ from the cell for one extracellular K+-ion.
D002463:Cell Membrane Permeability / Q000187:drug effects; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism; D007773:Lactates / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism
D007773:Lactates; D004024:Dicyclohexylcarbodiimide; D006859:Hydrogen; D011188:Potassium
Kharakter sekretsii laktata kletkami E. coli.