title: Factors affecting the production of hydrogenase by Desulfovibrio desulfuricans.
abstract: An examination of conditions for the growth of Desulfovibrio desulfuricans, with the aim of optimizing hydrogenase production, is reported. An ammonium sulfate-lactate-yeast extract medium gave 5 to 10 times as much hydrogenase activity as a peptone-yeast extract medium. It made little if any difference whether the gas used for sparging was nitrogen, hydrogen, or a mixture thereof but increasing the rate of sparging and agitation did result in a slight decrease in activity. Control of pH during culture development was of little benefit to hydrogenase production. At least two hydrogenases were present in D. desulfuricans: one periplasmic, the other membrane bound.
mesh_terms: D003470:Culture Media; D003593:Cytoplasm / Q000201:enzymology; D003901:Desulfovibrio / Q000201:enzymology* / Q000254:growth & development; D006859:Hydrogen / Q000378:metabolism / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006864:Hydrogenase; D008566:Membranes / Q000201:enzymology; D009584:Nitrogen / Q000494:pharmacology; D010088:Oxidoreductases / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D006859:Hydrogen; D010088:Oxidoreductases; D006864:Hydrogenase; D009584:Nitrogen