Determination of captopril in biological fluids by gas-liquid chromatography.
The quantitation of captopril in biological fluids by gas-liquid chromatography using a flame photometric detector is described. Captopril was converted into an adduct with N-ethylmaleimide and then into the hexafluoroisopropyl ester. The latter derivative was separated on a 2% OV-210 column and determined by employing the captopril-N-hexylmaleimide adduct as an internal standard. The blood level and urinary excretion of captopril administered intravenously to dogs were measured by the proposed method.
D000818:Animals; D002216:Captopril / Q000008:administration & dosage / Q000097:blood* / Q000652:urine; D002849:Chromatography, Gas / Q000379:methods; D004285:Dogs; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D011392:Proline / Q000031:analogs & derivatives*
D011392:Proline; D002216:Captopril