Genotoxicity of pyrrolizidine alkaloids in the hepatocyte primary culture/DNA-repair test.
The genotoxicity of 6 pyrrolizidine alkaloids and 2 related compounds was examined in the hepatocyte primary culture/DNA-repair test and compared to mutagenicity in a modified Salmonella/microsome test. All 6 pyrrolizidine alkaloids were positive in the DNA-repair test, and 4 of these are known to be carcinogenic. Of the 6 positive compounds, only 5 were mutagenic. Thus, the hepatocyte primary culture/DNA-repair test displays a comparable or better capacity than the bacterial mutagenesis test for detecting the genotoxic effects of this class of carcinogens.
D000818:Animals; D002478:Cells, Cultured; D004260:DNA Repair; D008099:Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens; D011763:Pyrrolizidine Alkaloids / Q000494:pharmacology*; D051381:Rats; D012486:Salmonella typhimurium / Q000235:genetics
D009153:Mutagens; D011763:Pyrrolizidine Alkaloids