Effect of trypsin treatment of rat adipocytes on biological effects and binding of insulin and insulin-like growth factors: further evidence for the action of insulin-like growth factors through the insulin receptor.
Trypsin-treatment of isolated rat adipocytes abolishes the metabolic effects not only of insulin, but also of the insulin-like growth factors: in trypsin-treated cells, concentrations of these hormones that are otherwise maximally effective no longer stimulate 3-O-methylglucose transport and lipogenesis or inhibit epinephrine induced lipolysis. Concomitantly, the trypsin-treated adipocytes no longer display specific insulin binding. In contrast, the characteristics of the binding of the insulin-like growth factors are not grossly affected by prior trypsinization of the adipocytes. These findings add further support to the concept that the insulin-like growth factors act on glucose metabolism and antilipolysis via the insulin receptor of the adipocyte.
D019325:3-O-Methylglucose; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism* / Q000494:pharmacology; D008066:Lipolysis / Q000187:drug effects; D008757:Methylglucosides / Q000378:metabolism; D010455:Peptides / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D011972:Receptor, Insulin / Q000187:drug effects* / Q000378:metabolism; D013002:Somatomedins / Q000378:metabolism* / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology*
D007328:Insulin; D008757:Methylglucosides; D010455:Peptides; D013002:Somatomedins; D019325:3-O-Methylglucose; D011972:Receptor, Insulin; D014357:Trypsin