Use of cir-lac operon fusions to study transcriptional regulation of the colicin Ia receptor in Escherichia coli K-12.
We describe cir-lac operon fusions constructed by using phage Mu d(Apr lac). Expression of beta-galactosidase in these fusion strains is analogous to known regulatory properties of cir gene expression. It is concluded that the observed regulation by iron of the cir gene is under transcriptional control.
D002951:Citrates / Q000494:pharmacology; D003087:Colicins / Q000378:metabolism; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D005290:Ferric Compounds / Q000494:pharmacology; D005786:Gene Expression Regulation; D005821:Genetic Techniques; D009876:Operon; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic; D014168:Transferrin / Q000494:pharmacology
D002951:Citrates; D003087:Colicins; D004274:DNA, Recombinant; D029968:Escherichia coli Proteins; D005290:Ferric Compounds; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D014168:Transferrin; C028593:colicin receptor, E coli