[Insulin and in vitro protein synthesis of isolated liver ribosomes in experimental acute uraemia (author's transl)].
Liver protein synthesis was shown to be enhanced in acute experimental uraemia, when intracellular amino acids were used as substrate. Since protein synthesis also depends on substrate availability and amino acid concentration is higher in uraemic liver cells, it was of interest to investigate the in vitro protein synthesis capacity of isolated liver ribosomes in the presence of standardized amino acids with and without in vivo insulin stimulation. It was demonstrated that protein synthesis capacity of isolated liver ribosomes remains unaltered in acute uraemia and can be stimulated by in vivo insulin application. Our data suggest that in acute uraemia, intracellular substrate availability plays the essential role in impaired liver protein synthesis.
D000208:Acute Disease; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism*; D014176:Protein Biosynthesis; D051381:Rats; D014511:Uremia / Q000378:metabolism*
D000596:Amino Acids; D007328:Insulin
Insulin und in-vitro-Proteinsynthese isolierter Leberribosomen bei akuter experimenteller Urämie.