Spontaneous antibody-secreting human blood lymphocytes detected with a protein A plaque assay.
Spontaneous antibody-secreting lymphocytes in human peripheral blood were detected and counted by a protein A plaque assay. The level of plaque-forming cells (PFC) in peripheral blood was significantly higher in patients with immune disorders (per 10(6) lymphocytes: IgG PFC, 2,654 +/- 684; IgA PFC, 1,727 +/- 327; IgM PFC, 403 +/- 92) than in normal controls (per 10(6) lymphocytes: IgG PFC, 327 +/- 56; IgA PFC 633 +/- 128; IgM PFC, 215 +/- 105). There was no direct correlation between the PFC numbers of different Ig classes.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000921:Antibody-Producing Cells; D006462:Hemolytic Plaque Technique; D006801:Humans; D007154:Immune System Diseases / Q000276:immunology; D007136:Immunoglobulins / Q000096:biosynthesis; D007958:Leukocyte Count; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D013205:Staphylococcal Protein A
D007136:Immunoglobulins; D013205:Staphylococcal Protein A