Isolation of a yeast centromere and construction of functional small circular chromosomes.
The centromeric DNA (CEN3) from yeast chromosome III has been isolated on a 1.6 kilobase-pair segment of DNA located near the centromere-linked CDC10 locus of Saccharomyces cerevisiae. When present on a plasmid carrying a yeast chromosomal replicator, CEN3 enables that plasmid to function as a chromosome both mitotically and meiotically. Minichromosomes containing CEN3 are stable in mitosis and segregate as ordinary yeast chromosomes in the first and second meiotic divisions.
D002503:Centromere / Q000648:ultrastructure*; D002875:Chromosomes / Q000648:ultrastructure*; D004261:DNA Replication; D004270:DNA, Circular / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics*; D008540:Meiosis; D008938:Mitosis; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004270:DNA, Circular; D004271:DNA, Fungal