Separation of solid tumor cell populations.
Separation of subpopulations of cells from mouse solid tumors has been achieved with two methods: centrifugal elutriation and equilibrium density centrifugation. Elutriation resulted in enrichment of normal cells, and of tumor cells in specific cell cycle phases. Density centrifugation appeared to separate the well-oxygenated from the chronically hypoxic cells. These methods have been used to determine in vivo the cell-cycle phase-dependent cytotoxicity of chemotherapeutic drugs toward tumor cells.
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation / Q000379:methods; D006224:Cricetinae; D003412:Cricetulus; D007399:Interphase; D051379:Mice; D009374:Neoplasms, Experimental / Q000188:drug therapy / Q000473:pathology*; D010100:Oxygen
D000970:Antineoplastic Agents; D010100:Oxygen