title: Hapten-specific blockade of CBA/N x C3H/HeN F1 B cell responses in a cell transfer system.
abstract: CBA/N mice harbor an X-linked B cell defect which is transmitted by CBA/N female mice to their hybrid male progeny. Hapten-specific plaque-forming cell (PFC) responses to haptenated proteins by B cell-defective CBA/N mice and CBA/N X C3H/HeN F1 male mice can be blockaded by concomitant exposure to polysaccharide agents bearing the same hapten. Various experimental approaches were explored in an attempt to study this phenomenon in a syngeneic cell transfer system. Unprimed donor spleen cells were unable to mount adequate PFC responses to dinitrophenylated-hemocyanin (DNP-KLH) in irradiated, syngeneic recipients. DNP-KLH-primed donor spleen cells produced strong PFC responses after challenge in irradiated recipients, and these secondary responses were subject to hapten-specific blockade by DNP-derivatized polysaccharide agents. Both direct and indirect PFC responses could be blocked. DNP conjugated to bovine serum albumin also produced hapten-specific blockade in this cell transfer system. Under some cell transfer conditions normal CBA/N X C3H/HeN F1 female spleen cells were just as susceptible to hapten-specific blockade as defective F1 male spleen cells.
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001667:Binding, Competitive; D002417:Cattle; D004136:Dinitrobenzenes / Q000276:immunology; D005260:Female; D005362:Ficoll / Q000276:immunology; D006241:Haptens; D006462:Hemolytic Plaque Technique; D007116:Immunization, Passive; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D011135:Polysaccharides, Bacterial / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000276:immunology; D013154:Spleen / Q000166:cytology
chemical_list: D004136:Dinitrobenzenes; D006241:Haptens; D011135:Polysaccharides, Bacterial; D005362:Ficoll; D012710:Serum Albumin, Bovine