Long term primary monolayer culture of adult murine magnocellular neurons.
Primary monolayer culture of adult murine hypothalamic cells has been carried out for 2 months. Neuron-like cells as well as fibroblast, glial, and ependymal-like cells demonstrated characteristic morphological features which distinguished them from one another. In addition, immunocytochemical identification of cytoplasmic substances cross-reactive with neurophysin and (8-arginine)vasopressin further characterized specific magnocellular neuronal populations throughout the culture period. This culture system should provide a basis for studying neurosecretion at the cellular and molecular levels.
D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis; D002369:Castration; D002478:Cells, Cultured; D005260:Female; D005455:Fluorescent Antibody Technique; D007031:Hypothalamus / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009481:Neurophysins / Q000032:analysis; D051381:Rats; D013997:Time Factors
D009481:Neurophysins; D001127:Arginine Vasopressin