title: tRNA methylation. A rapid and simple method for determination of total radioactivity and methylated base distribution in the same sample.
abstract: A simple and rapid method is described for the analysis of in vitro methylated tRNA. The total radioactivity is first determined after trichloroacetic acid-precipitation and filtration. Then the tRNA is hydrolyzed while still on the filter, and the bases are separated by thin-layer chromatography. This method eliminates the need for duplicate sample preparation, and has proved especially useful when the amount of tRNA and/or tRNA methylase is limited.
mesh_terms: D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer / Q000379:methods; D004926:Escherichia coli / Q000378:metabolism; D008745:Methylation; D012343:RNA, Transfer / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D012436:S-Adenosylmethionine / Q000378:metabolism; D012359:tRNA Methyltransferases / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D012436:S-Adenosylmethionine; D012343:RNA, Transfer; D012359:tRNA Methyltransferases