Fecal factors which modify the formation of fecal co-mutagens in high- and low-risk population for colon cancer.
Dietary patterns and fecal mutagens of 2 population groups with a distinct risk for colon cancer, high-risk, non-Seventh-Day Adventists and a low-risk, vegetarian Seventh-Day Adventists, in the New York metropolitan area were studied. The diet histories indicate that the intake of protein was identical in the 2 groups; a greater portion coming from meat in non-Seventh-Day Adventists and from vegetables in the Seventh-Day Adventists. The intake of fat was lower in Seventh-Day Adventists. Fecal samples were extracted with dichloromethane, partially purified on silica and assayed for mutagenic activity using the Salmonella/mammalian microsome mutagenicity test. When tested alone, fecal extracts from both groups were non mutagenic in TA98 and TA100 strains. Fecal extracts of non-Seventh-Day Adventists and Seventh-Day Adventists enhanced the mutagenic activity induced by 2-AAF in TA98 and TA100 (co-mutagenic activity). However, non-Seventh-Day Adventists showed a significantly higher co-mutagenic activity in TA98 than did Seventh-Day Adventists.
D015073:2-Acetylaminofluorene / Q000633:toxicity*; D004032:Diet; D004041:Dietary Fats; D004121:Dimethyl Sulfoxide / Q000633:toxicity*; D005243:Feces / Q000032:analysis*; D006801:Humans; D008297:Male; D008460:Meat; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens; D012306:Risk; D012486:Salmonella typhimurium / Q000187:drug effects
D004041:Dietary Fats; D009153:Mutagens; D015073:2-Acetylaminofluorene; D004121:Dimethyl Sulfoxide