In vitro synthesis of anti-mycobacterial antibodies in biopsies from skin lesions of leprosy patients.
To demonstrate local synthesis of anti-Mycobacterium leprae antibodies, biopsies from skin lesions of leprosy patients were cultured in vitro in a medium containing 14C-labeled lysine and isoleucine, and the culture fluids were analyzed by crossed immunoelectrophoresis with intermediate gel and autoradiography. The results show that anti-M. leprae antibodies were synthesized in vitro in the biopsies from the skin lesions of leprosy patients and that the specificity of the locally produced antibodies varied from patient to patient.
D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis*; D001706:Biopsy; D046508:Culture Techniques; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007918:Leprosy / Q000276:immunology* / Q000473:pathology; D009166:Mycobacterium leprae / Q000276:immunology*; D012867:Skin / Q000473:pathology
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G