title: Detection of regulatory receptors and distinctive surface markers.
abstract: Cell sorting has been used as an approach to describing surface markers and functional receptors of hemopoietic stem cells and progenitors. Significant enrichment of CFU-S was obtained with fluorescent cell sorting of human antihuman sperm-labelled bone marrow cells; significant enrichment of human CFU-C was obtained by cell sorting of hybridoma (KGP1)-labelled human bone marrow cells. Significant changes in human bone marrow CFU-C, but not CFU-E were observed by fluorescence polarization after exposure to a putative stimulator of CFU-C. A description of preliminary investigations of a hybrid clone from Dexter culture cells x Chinese hamster ovary and fluorescence polarization measurements after exposure to purified mouse-lung CSF are also presented.
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002469:Cell Separation / Q000379:methods*; D006224:Cricetinae; D003584:Cytological Techniques; D004912:Erythrocytes / Q000166:cytology; D005260:Female; D005454:Fluorescence Polarization; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006822:Hybrid Cells; D008297:Male; D051379:Mice; D013094:Spermatozoa / Q000276:immunology; D001613:beta 2-Microglobulin / Q000494:pharmacology
chemical_list: D000954:Antigens, Surface; D001613:beta 2-Microglobulin