An immunoanalysis for kojic acid.
An enzyme-linked immunosorbent assay (ELISA) was developed for the analysis of kojic acid in aqueous solutions and biological fluids. As little as 100 pg kojic acid ml-1 was detected in standard buffer. Analysis of biological fluids containing 1 mg kojic acid ml-1 showed that ELISA could detect the toxin at dilutions of 10(-4) in urine, deporteinated plasma, or serum. Urine and plasma samples collected form rabbits dosed intravenously with 56 mg kojic acid kg-1 body weight gave positive tests at dilutions of 10(-4). The immunoassay described may be used for detection of kojic acid produced during experimental or clinical aspergillosis.
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D009183:Mycotoxins / Q000032:analysis* / Q000276:immunology; D011714:Pyrans / Q000032:analysis*; D011753:Pyrones / Q000032:analysis* / Q000276:immunology; D011817:Rabbits
D009183:Mycotoxins; D011714:Pyrans; D011753:Pyrones; C011890:kojic acid