Enzyme immunoassay for specific determination of the synthetic estrogen, ethynyl estradiol, in plasma.
In this enzyme immunoassay for ethynyl estradiol, a conjugate of the 3-(O-carboxymethyl)ether with horseradish peroxidase is used as the label and a conjugate of the 6-(O-carboxymethyl)oxime with bovine serum albumin as the immunogen. A solid-phase antibody procedure is used in separating antibody-bound and free steroid. The lower limit of sensitivity of the assay is 2 pg per assay tube. Interference by synthetic progestagens was minimized by extracting the sample with an anti-estrogen serum before assay. Results obtained with a comparison radioimmunoassay and this procedure agreed well (r greater than 0.98).
D003276:Contraceptives, Oral; D004997:Ethinyl Estradiol / Q000097:blood*; D005260:Female; D006461:Hemolysis; D006735:Horseradish Peroxidase; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay / Q000379:methods
D003276:Contraceptives, Oral; D004997:Ethinyl Estradiol; D006735:Horseradish Peroxidase