Quantitative, radial diffusion slide assay for staphylocoagulase.
A simple, quantitative radial diffusion assay for staphylocoagulase in culture fluids, using microscope slides coated with a thin layer of agar containing plasma and fibrinogen, was developed. No prior purification of the enzyme was needed, and only small quantities, 7 microliter, were required for each test. This method is particularly suitable for objectively comparing the relative amounts of coagulase produced by different cultures of Staphylococcus aureus.
D000818:Animals; D001431:Bacteriological Techniques; D001769:Blood; D003030:Coagulase / Q000032:analysis* / Q000378:metabolism; D003470:Culture Media; D005260:Female; D011817:Rabbits; D013211:Staphylococcus aureus / Q000201:enzymology*; D013552:Swine
D003030:Coagulase; D003470:Culture Media