A microcapillary assay (MASS) for macrophage migration inhibition factor.
A technically simple microassay for macrophage migration inhibition studies is described. Using simple 0.08 ml of test solution per assay, meaningful statistical analysis via multiple replicates is possible. A novel feature is that test solutions may be stored frozen in the test well until the indicating macrophages are ready for use. The assay was used to demonstrate that MIF, derived from the human lymphoid cell line RPMI 1788, eluted from Sephadex G-75 in the molecular weight range of 13,000--25,000.
D000818:Animals; D002460:Cell Line; D002850:Chromatography, Gel; D006168:Guinea Pigs; D006801:Humans; D007158:Immunologic Techniques; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis; D008297:Male
D008263:Macrophage Migration-Inhibitory Factors