title: Pleiotropic effects of alkaline phosphatase regulatory mutations phoB and phoT on anaerobic growth of and polyphosphate synthesis in Escherichia coli.
abstract: The appearance during anaerobiosis of spontaneous phoT phoB double mutants in a phoT background is described. During both exponential growth and stationary phase, selection against the phoT mutants relative to the wild type was evident. This reduction in viability of phoT mutants was suppressed in phoT phoB double mutants. Sensitivity to anaerobiosis was shown to be correlated with polyphosphate overproduction. A possible pleiotropic function of phoT and phoB is suggested.
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics* / Q000502:physiology; D000693:Anaerobiosis; D004790:Enzyme Induction; D004926:Escherichia coli / Q000502:physiology*; D005809:Genes, Regulator; D005816:Genetic Complementation Test; D009154:Mutation; D011122:Polyphosphates / Q000096:biosynthesis*
chemical_list: D011122:Polyphosphates; D000469:Alkaline Phosphatase