[Induction of partial immune tolerance in a closely-related species system by a single injection of papain-extracted, donor species specific, lymphocyte membrane antigens].
This paper reports of experimental xenografting of kidneys in closely related species (fox-dog) modifying primary graft rejection by single i.v. injection of donorspecies-specific lymphocyte antigen in soluble form. Papain treatment was used to separate histocompatibility antigens from lymphocytes of donors. This antigen, in vitro, is capable of reducing cytotoxicity of xeno-antisera by adsorption of their lymphocytotoxic antibodies. A single intraoperative i.v. injection of this antigen to the transplant recipients results in the subsequent failure of humoral antibody production, thus this antigen-mediated effect is thought to be a state of B-cell tolerance. Survival times are more than doubled in comparison to untreated controls and last in average 14.6 +/- 1.74 days (n = 5). Histological studies demonstrate rather well conserved renal parenchym with sporadic infiltrations of round cells.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D004285:Dogs / Q000276:immunology*; D005260:Female; D005589:Foxes / Q000276:immunology*; D006084:Graft Rejection; D006649:Histocompatibility Antigens / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D007108:Immune Tolerance; D007668:Kidney / Q000166:cytology; D016030:Kidney Transplantation; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D010206:Papain; D013045:Species Specificity; D014183:Transplantation, Heterologous
D000954:Antigens, Surface; D006649:Histocompatibility Antigens; D010206:Papain
Induktion partieller Immunotoleranz im nah verwandten Speziessystem durch einmalige Injektion eines mit Papain gewonnenen, spenderspezies-spezifischen Lylmphozytenmembran-Antigens.