Concentration of hemopoietic stem cells using a light-activated cell sorter.
Light-activated cell sorting although capable of highly specific selection of subpopulations of cells is rate-limited. The method as it now exists is insufficient to supply an adequate number of stem cells for bone marrow transplantation. However, other separation methods using electrophoresis, density gradients, and density centrifugation coupled with cell sorting can improve the yield for clinical utilization.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D003584:Cytological Techniques; D004586:Electrophoresis; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D008027:Light; D051379:Mice; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D013499:Surface Properties
D000954:Antigens, Surface