The detection of islet-cell antibodies by immunofluorescence in Bouin's-fixed, paraffin-embedded human pancreas.
Pancreatic islet-cell antibodies have been demonstrated in the sera of insulin-dependent diabetic patients, with or without polyendocrine disease, by immunofluorescent microscopy in which cryostat sections of fresh-frozen human pancreas were used as the substrate. In the present study we used sections of Bouin's-fixed, paraffin-embedded, trypsin-treated human pancreas as the islet-cell substrate and compared the results to those obtained with cryostat sections. Twenty-four sera from insulin-dependent diabetic patients, one serum from a non-insulin dependent diabetic patient and five sera from normal individuals were tested. With serum samples containing islet- cell antibodies, Bouin's-fixed, paraffin-embedded tissue produced a more intense fluorescence, was easier to use and was more sensitive than the conventional cryostat method.
D000085:Acetates; D019342:Acetic Acid; D000906:Antibodies / Q000032:analysis*; D003584:Cytological Techniques; D003920:Diabetes Mellitus / Q000276:immunology; D005404:Fixatives; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde; D006801:Humans; D007515:Islets of Langerhans / Q000187:drug effects / Q000276:immunology*; D010179:Pancreas / Q000276:immunology*; D010232:Paraffin; D010851:Picrates; D014357:Trypsin / Q000494:pharmacology
D000085:Acetates; D000906:Antibodies; D005404:Fixatives; D010851:Picrates; D005557:Formaldehyde; C026239:Bouin's solution; D010232:Paraffin; D014357:Trypsin; D019342:Acetic Acid