Outer membrane proteins of Shigella sonnei. I. Characterization of phase I, phase II and R-form Shigella sonnei.
The cell envelope of Shigella sonnei phase I, phase II and R-form was fractionated inot outer and cytoplasmic membrane components by means of sucrose density gradient centrifugation. The protein composition of the outer membrane has been analyzed by SDS-polyacrylamide gel electrophoresis. The outer membrane preparations contained 15-17 proteins. The major proteins of the outer membrane were of apparent molecular weights: 27,000, 28,000 and 31,000. Their amount varied depending on the structural defects of lipopolysaccharide.
D001426:Bacterial Proteins / Q000302:isolation & purification*; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008565:Membrane Proteins / Q000302:isolation & purification*; D008970:Molecular Weight; D009154:Mutation; D012764:Shigella sonnei / Q000166:cytology*
D001426:Bacterial Proteins; D008565:Membrane Proteins