[Oxidative phosphorylation in Mycobacterium lepraemurium].
The generation of ATP by cell-free extracts of Mycobacterium lepraemurium isolated from Sprague-Dawley rats was investigated. Cell-free preparations catalyzed phosphorylation coupled to the oxidation of NADH and succinate yielding P/O ratios of 0.6 and 0.4, respectively. Ascorbate oxidation did not result in ATP formation. The oxidative phosphorylation was uncoupled by 2,4- dinitrophenol and pentachlorophenol. Phosphate esterification coupled to NADH oxidation was inhibited by rotenone which had no effect on ATP synthesis associated with succinate oxidation. Antimycin A and cyanide completely inhibited phosphorylation coupled to the oxidation of NADH or succinate.
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D003486:Cyanides / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D009167:Mycobacterium lepraemurium / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D010416:Pentachlorophenol / Q000494:pharmacology; D051381:Rats; D013386:Succinates / Q000378:metabolism
D003486:Cyanides; D004140:Dinitrophenols; D013386:Succinates; D009243:NAD; D000968:Antimycin A; D000255:Adenosine Triphosphate; D010416:Pentachlorophenol
Phosphoryoation oxydative chez Mycobacterium lepraemurium.