Characterization of coronavirus-infected tissue cultures by computer-aided cytophotometry.
Coronavirus-infected cells were cytophotometrically measured and analyze during a replication cycle. Distinct cytoplasmic differences were observed in images scanned at 260 and 280 nm; these differences could be attributed to specific virologic changes using virologic tests. The application of computer-aided cytophotometry may prove to be a valuable approach in the analysis of infected cells.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D003201:Computers; D003332:Coronaviridae / Q000502:physiology*; D003584:Cytological Techniques; D003588:Cytopathogenic Effect, Viral; D003593:Cytoplasm / Q000382:microbiology / Q000648:ultrastructure; D012367:RNA, Viral / Q000096:biosynthesis; D012513:Sarcoma, Experimental
D012367:RNA, Viral