Leukocyte migration in different systems. Effect of colchicin. Kinetics of migration under agarose.
In a double-filter modification of Boyden's method, formylmethionylleucylphenylalanine (FMLP) needed the presence of fresh plasma to induce leukocyte locomotor kinetics which compared with a model for directional migration. FMLP, or zymosan-activated plasma, did not stimulate migration in capillary tubes. The kinetics of leukocyte migration under agarose towards zymosan-activated plasma were more compatible to a model for increased random motility than for directional migration. Treatment of leukocytes with colchicin reduced their migration under gradient and non-gradient conditions in millipore filters, capillary tubes, and in under-agarose experiments.
D001769:Blood; D002465:Cell Movement; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology*; D003584:Cytological Techniques; D006801:Humans; D007700:Kinetics; D007962:Leukocytes / Q000502:physiology*; D008433:Mathematics; D009239:N-Formylmethionine / Q000031:analogs & derivatives / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009842:Oligopeptides / Q000494:pharmacology; D012685:Sepharose; D015054:Zymosan / Q000494:pharmacology
D009842:Oligopeptides; D009239:N-Formylmethionine; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan; D012685:Sepharose; D003078:Colchicine