[Organelle-specific enzymatic activity as an index in evaluating the quality of liver preservation].
The dynamics of changes of the general, "non-sedimenting" activity of organelle-specific enzymes as well as their activity in perfusate during hypothermal conservation of the liver were studied in 77 experiments by the method of continuous and discontinuous perfusion, without perfusion and during the period of normal thermally artificial blood circulation after 3 hours of the organ conservation. The complex of enzyme tests including the assessment of "non-sedimenting activity" of mitochondrial glutamatdehydrogenase and lysosomal acid desoxiribonuclease during the conservation period was found to be an adequate criterion of the liver conservation quality. After the recovery of the artificial blood flow in the conservated organ the conservation quality is also assessed by their activity in the perfusate, in addition to determination of urokinase activity in the perfusate.
D000818:Animals; D003593:Cytoplasm / Q000201:enzymology; D004285:Dogs; D004789:Enzyme Activation; D008099:Liver / Q000201:enzymology*; D016031:Liver Transplantation; D008247:Lysosomes / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D009940:Organoids / Q000201:enzymology*; D013696:Temperature; D013997:Time Factors; D014021:Tissue Preservation; D014022:Tissue Survival
Aktivnost' organellospetsifichnykh fermentov kak pokazatel' otsenki kachestva konservatsii pecheni.