Azidopolynucleotides as photoaffinity reagents.
Polynucleotides containing adenosine and 8-azidoadenosine or inosine and 8-azidoinosine residues have been prepared from mixtures of nucleoside diphosphates using polynucleotide phosphorylase from Escherichia coli. These copolymers can form complexes with polyuridylic or polycytidylic acids respectively. Single stranded poly(adenylic, 8-azidoadenylic acid) [poly(A,z8A)] has been used as a photoaffinity reagent to explore the subunit topography of RNA polymerase from E. coli.
D000241:Adenosine; D000249:Adenosine Monophosphate / Q000031:analogs & derivatives*; D000345:Affinity Labels; D001386:Azides; D001665:Binding Sites; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D007288:Inosine; D007291:Inosine Monophosphate / Q000031:analogs & derivatives; D007292:Inosine Nucleotides; D007700:Kinetics; D011066:Poly C; D011072:Poly U; D011117:Polyribonucleotide Nucleotidyltransferase; D011131:Polyribonucleotides; D011485:Protein Binding; D014158:Transcription, Genetic
D000345:Affinity Labels; D001386:Azides; D007292:Inosine Nucleotides; D011131:Polyribonucleotides; D007291:Inosine Monophosphate; D011072:Poly U; D011066:Poly C; D000249:Adenosine Monophosphate; D007288:Inosine; C021291:8-azidoadenosine 5'-monophosphate; C027191:8-azidoinosine 5'-monophosphate; D012321:DNA-Directed RNA Polymerases; D011117:Polyribonucleotide Nucleotidyltransferase; D000241:Adenosine