An improved assay for proteases and polysaccharidases employing a cartilage proteoglycan substrate entrapped in polyacrylamide particles.
D000178:Acrylamides; D000818:Animals; D002356:Cartilage / Q000378:metabolism*; D002417:Cattle; D004396:Coloring Agents; D006026:Glycoside Hydrolases / Q000032:analysis*; D008751:Methylene Blue / Q000031:analogs & derivatives; D008863:Microspheres; D009666:Nose; D010447:Peptide Hydrolases / Q000032:analysis* / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism*; D014574:Uronic Acids / Q000032:analysis
D000178:Acrylamides; D004396:Coloring Agents; D011509:Proteoglycans; D014574:Uronic Acids; D006026:Glycoside Hydrolases; D010447:Peptide Hydrolases; D008751:Methylene Blue; C016401:1,9-dimethylmethylene blue