The value of simple lipid stains for typing skeletal muscle fibres.
Skeletal muscle fibre types can be distinguished rapidly with simple lipid stains. Comparative studies showed that Sudan Black B is superior to Oil Red O for this purpose and that optimum staining is obtained using unfixed section or sections fixed in calcium-glutaraldehyde. Factors that possibly influence the staining reaction, such as freeze-thawing, are considered. The stained lipids were identified by thin layer chromatography.
D000818:Animals; D001391:Azo Compounds; D002855:Chromatography, Thin Layer; D004396:Coloring Agents; D006652:Histological Techniques; D008055:Lipids / Q000032:analysis*; D008297:Male; D009132:Muscles / Q000166:cytology*; D009281:Naphthalenes; D009284:Naphthols; D009928:Organ Specificity; D051381:Rats
D001391:Azo Compounds; D004396:Coloring Agents; D008055:Lipids; D009281:Naphthalenes; D009284:Naphthols; C016118:Sudan Black B; C011049:oil red O