Propidium iodide as a nuclear marker in immunofluorescence. II. Use with cellular identification and viability studies.
The use of a fluorescence method employing propidium iodide to determine nuclear morphology of different cell types by epi-illumination in membrane immunofluorescence is described. Its usefulness is illustrated by differentiating nucleated and anuclear transferrin receptor-bearing cells as well as by simultaneously determining the viability of cell suspension without requiring a change from fluorescence microscopy, this causing no loss of visual accommodation.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002470:Cell Survival; D005312:Fetal Blood / Q000166:cytology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007231:Infant, Newborn; D008214:Lymphocytes / Q000378:metabolism*; D010617:Phenanthridines / Q000378:metabolism*; D011419:Propidium / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D014168:Transferrin / Q000276:immunology
D010617:Phenanthridines; D014168:Transferrin; D011419:Propidium