Differential role of Zn2+ in antigen- and mitogen-induced lymphokine production.
The effect on human lymphokine production in viro of phenanthroline, a Zn2+-chelating agent and an inhibitor of carboxypeptidases A and B, was tested. The elaboration of leucocyte migration inhibitory factor (LIF) by tuberculin-sensitized mononuclear cells stimulated with the specific antigen was reduced in a dose-dependent manner, an effect completely restored by addition of excess Zn2+. In contrast, phenanthroline did not affect LIF production by mononuclear cells activated nonspecifically by phytohaemagglutinin. It is hypothesized that the presence of a Zn2+-dependent molecule, possibly a carboxypeptidase, may be necessary for antigen- but not for mitogen-induced lymphokine production.
D000941:Antigens / Q000276:immunology; D006801:Humans; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis*; D008222:Lymphokines / Q000096:biosynthesis*; D008934:Mitogens / Q000276:immunology / Q000494:pharmacology; D009000:Monocytes / Q000276:immunology; D010618:Phenanthrolines / Q000276:immunology* / Q000494:pharmacology; D014373:Tuberculin / Q000276:immunology; D015032:Zinc / Q000276:immunology* / Q000494:pharmacology
D000941:Antigens; D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D008934:Mitogens; D010618:Phenanthrolines; D014373:Tuberculin; D015032:Zinc