Gel chromatographic separation of human C-peptide and proinsulin.
The immunoreactivity of circulating C-peptide is separated into two main peaks on a Bio-Gel column; the faster peak should not be proinsulin but an associated C-peptide without a covalent bond. Proinsulin is in fact eluted in the fraction prior to the faster eluting peak of C-peptide immunoreactivity with 1 M acetic acid as the eluting buffer. Therefore the use of gel chromatography to study C-peptide and proinsulin needs to be carefully re-evaluated, although the method has been established as one of the standard methods.
D002096:C-Peptide / Q000302:isolation & purification*; D002850:Chromatography, Gel / Q000379:methods; D005951:Glucose Tolerance Test; D006801:Humans; D007201:Indicator Dilution Techniques; D007330:Insulin Antibodies / Q000032:analysis; D010455:Peptides / Q000302:isolation & purification*; D011384:Proinsulin / Q000302:isolation & purification*
D002096:C-Peptide; D007330:Insulin Antibodies; D010455:Peptides; D011384:Proinsulin