title: Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae.
abstract: The complete nucleotide sequence of the actin gene from Saccharomyces cerevisiae has been determined. The coding region is interrupted by a 304-base-pair intervening sequence that is located within the triplet coding for amino acid 4. DNA sequences of the intron-exon junctions are similar to those found in higher eukaryotes and can be aligned such that the intron starts with the dinucleotide 5'-G-T-3' and ends with 5'-A-G-3'. Regions fo homology within the sequences upstream from the initiation codon and those following the termination codon have been detected between the yeast iso-1-cytochrome c gene and the actin gene. As deduced from the nucleotide sequence, yeast actin has 374 amino acid residues. Its primary structure, especially the NH2-terminal third of the protein, is highly conserved during evolution.
mesh_terms: D000199:Actins / Q000235:genetics*; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D003062:Codon; D004274:DNA, Recombinant; D005796:Genes; D009694:Nucleic Acid Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D000199:Actins; D000596:Amino Acids; D003062:Codon; D004274:DNA, Recombinant; D009694:Nucleic Acid Precursors; D012333:RNA, Messenger