Peptide map comparisons of epidermal and spleen H-2 molecules.
Peptide map comparisons of molecules encoded in the mouse H-2 complex isolated from epidermal cell preparations have been carried out. We previously showed that the Ia molecules from both the I-A and I-E subregions are synthesized by nonlymphoid bone-marrow-derived cells, probably Langerhans cells. The K and D or transplantation molecules are synthesized by both "true" epidermal cells and nonlymphoid bone-marrow-derived cells. The tryptic maps generated by separating tryptic peptides by high pressure liquid chromatography (HPLC) of epidermal H-2 molecules are identical to their spleen-cell counterparts. The biological significance of this finding is discussed.
D000818:Animals; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid; D004817:Epidermis / Q000276:immunology*; D005815:Genetic Code; D006183:H-2 Antigens / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D051379:Mice; D008805:Mice, Inbred A; D010455:Peptides / Q000276:immunology*; D011487:Protein Conformation; D013154:Spleen / Q000276:immunology*; D001613:beta 2-Microglobulin / Q000276:immunology
D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; D010455:Peptides; D001613:beta 2-Microglobulin