Histometric and fine-structural analysis of pig glomeruli after experimental protracted shock.
Glomeruli of control and shock-treated, 6-8-week-old pigs (n = 16 specific pathogen-free) were analyzed by light microscopy, transmission electron microscopy and morphometry. In eight of the animals the shock was induced using a neurotoxin of the E. coli serotype O 139: k82 (B). The animals were killed 47 to 264 hours post injection. In semithin sections a significant mesangial widening and increase of the mesangial nuclei count was demonstrated. The volume of the mesangium was 11.1% in the control animals and increased to 19.6% after protracted shock. By electron microscopy we ascertained a distinct activation of the mesangial cells concomitant with an increase of ribosomes, rough endoplasmatic reticulum, lysosomes and a fusion of the epithelial pedicles. The surrounding mesangial matrix was enlarged. In this paper the significance of this mesenchymal reaction in relation to shock situations is discussed.
D000818:Animals; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D004926:Escherichia coli; D007678:Kidney Glomerulus / Q000648:ultrastructure*; D008247:Lysosomes / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009498:Neurotoxins; D012270:Ribosomes / Q000648:ultrastructure; D012769:Shock / Q000139:chemically induced / Q000473:pathology*; D013552:Swine; D013997:Time Factors
D009498:Neurotoxins