Interaction of proteinases and their inhibitors from yeast. Activation of carboxypeptidase Y.
In a crude extract of baker's carboxypeptidase Y is predominantly found in an inactive form. A procedure for the isolation of the inactive form of the enzyme is presented. It is shown that the inactive form is identical to the reconstituted complex of carboxypeptide Y with its inhibitor. This complex is stable above pH 5, i.e., it remains inactive between pH 5 and 9. The conversion to the active enzyme occurs below pH 5, also in the absence of proteolytic enzymes. The inhibitor of carboxypeptidase Y can be removed enzymatically from the complex by treatment with proteinase B (EC 3.4.22.9) at pH 7. At pH 5, the carboxypeptidase Y-inhibitor complex is activated both by proteinase A (EC 3.4.23.6) and B. Yeast proteinases are activated in a crude extract by incubation at pH 5 [3]. Based on the levels of proteinase A and B in an activated extract and on the time required for conversion to active carboxypeptidase Y, proteinase B is at least 10-times more effective than proteinase A. Peptides that arise during the pH 5-incubation procedure did not accelerate the proteolytic activation of carboxypeptidase Y. The inhibitor of carboxypeptidase Y is completely degraded in the proteolytic activation steps, no accumulation of intermediates is observed. Only one form of active carboxypeptidase Y is found to be present in the proteolytically activated extracts, i.e., no polypeptide fragments of carboxypeptidase Y-inhibitor remain bound to the enzyme after it has been activated by proteinase B. In vacuoles prepared from spheroplasts no inactive carboxypeptidase Y can be detected.
D016282:Aspartic Acid Endopeptidases; D002268:Carboxypeptidases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D043402:Cathepsin A; D002852:Chromatography, Ion Exchange; D003600:Cytosol / Q000201:enzymology; D004146:Dioxanes; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010436:Pepstatins / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases; D014617:Vacuoles / Q000201:enzymology
D004146:Dioxanes; D010436:Pepstatins; D029701:Saccharomyces cerevisiae Proteins; D002268:Carboxypeptidases; D010450:Endopeptidases; D043402:Cathepsin A; C499283:PRC1 protein, S cerevisiae; C022633:serine carboxypeptidase; D012697:Serine Endopeptidases; C023302:yeast proteinase B; C022325:aspartic proteinase A; C499267:PEP4 protein, S cerevisiae; D016282:Aspartic Acid Endopeptidases