Characterization of insulin biosynthesis and secretion of pancreatic islets prepared from hyperglycaemic sand rats. II. Investigations of islets after different periods of cultivation.
Cultivation of pancreatic islets of sand rats in the presence of high glucose led to a very high insulin release and to strongly diminished insulin contents independent of the metabolic stage of the sand rats. Changes in the [3H]-leucine incorporation into (pro)insulin were not found in dependence of cultivation.
D000818:Animals; D003411:Arvicolinae; D002478:Cells, Cultured; D006943:Hyperglycemia / Q000378:metabolism*; D007328:Insulin / Q000096:biosynthesis / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D007700:Kinetics; D011384:Proinsulin / Q000096:biosynthesis
D007328:Insulin; D011384:Proinsulin