Enkephalin degradation stimulated by captopril.
Changes in the rates of degradation of Met- and Leu-enkephalin by brain aminopeptidase were measured by UV absorption after high-pressure liquid chromatography. The cleavage of enkephalin paralleled the generation of tyrosine. Captopril (SQ 14,225) stimulated enzyme activity, to a greater extent with Met- than with Leu-enkephalin. Pro-His-Pro-NH2, histidine, and histamine stimulated enkephalin aminopeptidase approximately twice as much as captopril. The sulfhydryl group of captopril was not the only factor in its effect. The addition of captopril did not change the pH optimum, and it stimulated enkephalin degradation between 25 and 50 degrees C.
D000626:Aminopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002216:Captopril / Q000031:analogs & derivatives / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004723:Endorphins / Q000378:metabolism*; D004745:Enkephalins / Q000378:metabolism*; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D011392:Proline / Q000031:analogs & derivatives*; D051381:Rats
D004723:Endorphins; D004745:Enkephalins; D011392:Proline; D002216:Captopril; D000626:Aminopeptidases