title: Chemical carcinogen in vitro testing: a method for sizing cell nuclei in the nuclear enlargement assay.
abstract: The observation that cells often respond to carcinogens by nuclear enlargement has suggested that this property might be useful to develop a short-term screening test for such compounds. Previous methods for detecting nuclear size increases have used an image analyzer system to detect nuclear changes in individual cells. This paper details a more rapid method for obtaining nuclei by use of a stromalyzing procedure following by analysis of nuclear volumes, using a Coulter Counter Channelyzer. This new and simplified nuclear sizing method should facilitate the use of the assay as a possible carcinogenesis screen by permitting rapid and efficient testing of large numbers of compounds.
mesh_terms: D002273:Carcinogens; D002452:Cell Count; D002467:Cell Nucleus / Q000187:drug effects*; D003513:Cycloheximide / Q000633:toxicity; D003520:Cyclophosphamide / Q000633:toxicity; D003584:Cytological Techniques; D004305:Dose-Response Relationship, Drug; D004996:Ethidium / Q000633:toxicity; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008466:Mechlorethamine / Q000633:toxicity; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D013997:Time Factors
chemical_list: D002273:Carcinogens; D008769:Methylnitronitrosoguanidine; D008466:Mechlorethamine; D003520:Cyclophosphamide; D003513:Cycloheximide; D004996:Ethidium