Search for viruses in rheumatoid macrophage-rich synovial cell populations.
The adherent cells remaining after short-term culture of synovial fluid and synovial membrane cells from rheumatoid and non-rheumatoid patients were examined for the presence of a productive virus infection and for various viral antigens. Labelling was carried out with 3H-thymidine and 3H-uridine followed by sucrose density gradient centrifugation of the culture supernatant. Only in 1 case was there incorporation of 3H-uridine into material of density 1 . 21 g/cm3. Viral antigens were tested for by indirect immunofluorescence with antisera to rubella virus, the retroviruses RD-114 and simian sarcoma associated virus, early adenovirus type 2 antigens, late adenovirus type 2 antigens, SV-40 T antigen, and in 1 case measles virus. No cell showed immunofluorescence with any antiserum except the early adenovirus type 2 antiserum, which stained the cytoplasm of about half the synovial cell cultures, some from rheumatoid and some from non-rheumatoid patients.
D000956:Antigens, Viral / Q000032:analysis; D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000382:microbiology*; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006801:Humans; D008264:Macrophages / Q000276:immunology / Q000382:microbiology*; D013582:Synovial Fluid / Q000166:cytology; D013583:Synovial Membrane / Q000382:microbiology*; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism; D014780:Viruses / Q000302:isolation & purification* / Q000378:metabolism
D000956:Antigens, Viral; D013936:Thymidine; D014529:Uridine