Effect of the cryopreservation on the production of leukocyte migration inhibition factor (LIF) by lymphocytes from normal volunteers and patients with laryngeal carcinomas.
The present paper reports the results of an investigation on the influence of cryopreservation methods of human lymphocytes on the leukocyte migration inhibition factor (LIF) production following PHA stimulation. The optimal freezing and thawing procedures, fetal calf serum and dimethyl sulphoxide concentrations are reported.
D001793:Blood Preservation; D005615:Freezing; D006801:Humans; D007822:Laryngeal Neoplasms / Q000276:immunology*; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis*; D008214:Lymphocytes / Q000276:immunology*; D008222:Lymphokines / Q000096:biosynthesis*
D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines