A new solid-phase radioimmunoassay to measure IgG secreted by cultured human lymphocytes.
We describe a simple solid-phase radioimmunoassay (RIA) to detect IgG based on competitive binding between radiolabeled and unlabeled IgG for anti-IgG antibody physically adsorbed to the wells of polyvinyl microtiter plates. The assay is sensitive (1 ng), rapid, and is particularly suited for studies of in vitro IgG secretion by human peripheral blood lymphocytes, since such studies require large numbers of cultures. Conditions which permit measurement, by means of this assay, of helper and suppressor T cell effects on IgG production by human B cells in culture are described.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D001667:Binding, Competitive; D002478:Cells, Cultured; D006801:Humans; D007074:Immunoglobulin G; D007163:Immunosorbent Techniques; D008214:Lymphocytes / Q000378:metabolism*; D010586:Phagocytes / Q000378:metabolism; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D012397:Rosette Formation
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G