title: The influence of cigarette tobacco smoke products on the immune response. The cellular basis of immunosuppression by a water-soluble condensate of tobacco smoke.
abstract: The immunosuppression exhibited by a water-soluble condensate of tobacco smoke (WSC) has been studied in vivo and in vitro. When multiple sublethal doses of WSC were injected into C57Bl/6 mice, their ability to respond to immunization with sheep erythrocytes by the formation of plaque-forming cells was severely inhibited. In addition, spleen cells from WSC-treated mice were unable to mount a primary response to SRBC in vitro. Studies on the cellular basis of the immunosuppression induced by WSC showed a decrease in T lymphocytes in the spleens of WSC-treated mice. Additional experiments were conducted in which isolated populations of T cells, B cells and macrophages from WSC-treated or normal mice were combined and then tested for responsiveness to SRBC in vitro. Results of these experiments also indicated that T cells were particularly susceptible to WSC exposure. T cells from WSC-treated mice were unable to co-operate with normal B cells and macrophages in the response to SRBC. A less marked suppression of B-cell function was noted in condensate-treated mice. While B cells from such animals were able to co-operate with normal T cells and macrophages to give a detectable primary response to SRBC, the response was depressed. In contrast, macrophages from WSC-treated animals enhanced the response of normal T and B cells to SRBC.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells; D001402:B-Lymphocytes / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007165:Immunosuppression Therapy; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010947:Plants, Toxic; D012906:Smoke; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014026:Nicotiana / Q000276:immunology*
chemical_list: D012906:Smoke