Use of thiotriphosphates for the study of in vitro initiation in adenovirus-infected cell nuclei.
The use of thiotriphosphates as a means of analyzing in vitro initiation products of adenovirus-infected cells is demonstrated for the RNA polymerase III transcript VA-RNA. Results suggest its usefulness in discriminating between adenosine- or guanosine-initiated transcripts and in selecting the 5'-oligonucleotide.
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D000256:Adenoviridae / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D006367:HeLa Cells; D012319:RNA Polymerase II / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis; D013873:Thionucleotides / Q000378:metabolism*; D014158:Transcription, Genetic
D012367:RNA, Viral; D013873:Thionucleotides; C022571:adenosine 5'-O-(3-thiotriphosphate); D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D012319:RNA Polymerase II