title: Isolation of an azide mutagenic metabolite in Salmonella typhimurium.
abstract: A scheme that employs a cation-exchange column and high-pressure liquid chromatography (HPLC) is devised to isolate and process large quantities of azide metabolite produced by S. typhimurium TA1530 strain. The mutagenic metabolite adheres strongly to the cation-exchange column, thus providing a convenient way to separate the metabolite from unreacted azide (N3-). The metabolite is very polar and only sparingly soluble in most organic solvents. Recrystallization in a methanol-carbon tetrachloride solvent system gave rise to microcrystalline material that decomposes with charring and gas evolution at 173-176 degrees C. The infrared spectrum indicates the presence of a covalently bound azide moiety.
mesh_terms: D001386:Azides / Q000302:isolation & purification* / Q000378:metabolism; D001711:Biotransformation; D009153:Mutagens / Q000302:isolation & purification*; D012486:Salmonella typhimurium / Q000032:analysis* / Q000378:metabolism; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001386:Azides; D009153:Mutagens