Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates.
The expression of cloned tRNA genes has been studied by injecting single-stranded and double-stranded DNA templates into Xenopus oocyte nuclei. In both forms the genes are faithfully transcribed after injection. Some single-stranded DNA is converted into double-stranded DNA in the oocyte nucleus. This conversion is necessary for the expression of the injected tRNA gene: no tRNA transcription is observed when DNA synthesis is inhibited. We conclude that single-stranded DNA does not serve as a template for faithful transcription of this gene in injected oocytes.
D003090:Coliphages / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D010957:Plasmids; D012343:RNA, Transfer / Q000235:genetics*; D013698:Templates, Genetic; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D012343:RNA, Transfer