A new assay for lytic anti-galactocerebroside (GC) antibodies employing 56Rubidium release from GC-labelled liposomes.
This assay is a quick and sensitive method for determination of antibodies to galactocerebroside (GC). The use of antibody-plus-complement radioisotope release from GC-bearing liposomes allows numerous samples to be handled in semi-automated fashion. By substituting alternative gangliosides or cerebrosides, the technique may be simply modified for assay of antibodies to other substances.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D002554:Cerebrosides / Q000276:immunology*; D003165:Complement System Proteins / Q000276:immunology; D005699:Galactosylceramides / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques; D008081:Liposomes / Q000276:immunology; D011817:Rabbits; D011868:Radioisotopes; D012413:Rubidium
D000906:Antibodies; D002554:Cerebrosides; D005699:Galactosylceramides; D008081:Liposomes; D011868:Radioisotopes; D003165:Complement System Proteins; D012413:Rubidium