[Effect of the ts 15 mutation of transcription termination factor rho on expression of the uridine phosphorylase gene of Escherichia coli].
The expression of nucleoside-catabolizing genes was studied in two isogenic strains, SA1030(rho+) and AD1600(rho ts 15). The deo-enzymes activities do not differ markedly in these two strains, though when cells were grown on glycerol medium the deo-enzymes level appeared to be higher than on glucose medium. The synthesis of uridine phosphorylase in AD1600 strain was found to be constitutive. It was found also that the cytR regulation system was not altered in AD1600 strain and a wild-type allele of udp gene was present in the genome of AD1600 strain. A conclusion is made that constitutive synthesis of uridine phosphorylase is due to the presence of rho ts 15 mutation in the genome of the strain AD1600.
D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005786:Gene Expression Regulation; D009154:Mutation; D010430:Pentosyltransferases / Q000235:genetics*; D012234:Rho Factor / Q000235:genetics* / Q000502:physiology; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D014543:Uridine Phosphorylase / Q000096:biosynthesis / Q000235:genetics*
D012234:Rho Factor; D014157:Transcription Factors; D010430:Pentosyltransferases; D014543:Uridine Phosphorylase
Vliianie mutatsii po faktoru terminatsii transkriptsii rho ts 15 na vyrazhenie uridinfosforilaznogo gena u Escherichia coli.