Immunochemical demonstration of immunoreactive insulin in human breast cancer.
The immunoreactive insulin contents of the cytosol extracts of 57 human mammary carcinomas were measured by specific radioimmunoassay. All of these extracts contained measurable insulin (detection limit 1.3 ng/g tumor). In some cases (23) the levels were quite greater than the normal human serum levels. Immunoperoxidase technique revealed the presence of immunoreactive insulin within the cytoplasm of tumor cells in 6 cases.
D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000378:metabolism*; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007328:Insulin / Q000378:metabolism*; D007330:Insulin Antibodies / Q000032:analysis; D008875:Middle Aged; D011863:Radioimmunoassay; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism
D007328:Insulin; D007330:Insulin Antibodies; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone