Chromosome delineation induced by a combined potassium permanganate-sodium bisulfite treatment.
A cytological procedure for in vitro chromosome delineation has been studied using human and mouse (Mus musculus) chromosomes. This method, consisting of slide incubation in KMnO4 at 0--5 degrees C for 24 h followed by a short exposure to NaHSO3 (1--3 min) and Giemsa staining, induces extraction of chromatin from human and mouse interphase nuclei and chromosomes. Autoradiography after 3H-ThD incorporation in vitro and cytophotometry confirmed that DNA is removed. Well contour-delineated and non-distorted chromosomes are observed in both species allowing the identification of all human chromosome groups. Contour chromosome delineation and its relationship to chromosome organization is briefly discussed.
D000818:Animals; D001345:Autoradiography; D001853:Bone Marrow / Q000648:ultrastructure; D002478:Cells, Cultured; D002875:Chromosomes / Q000648:ultrastructure*; D003584:Cytological Techniques; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D051379:Mice; D011196:Potassium Permanganate; D013447:Sulfites; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D013447:Sulfites; D011196:Potassium Permanganate; D014316:Tritium; D013936:Thymidine