Digoxin sensitivity in amyloid cardiomyopathy.
Digoxin (5 mg/ml) was added to 10-mg and 20-mg pellets of purified primary and secondary amyloid fibrils, a normal human liver and heart homogenate and a homogenate from the heart of a patient with amyloid cardiomyopathy who had not received digitalis. After centrifugation, the supernatants were recovered and assayed for digoxin concentrations. Aliquots from the sediments were studied for the presence of digoxin, using rabbits antidigoxin antiserum and an indirect immunofluorescent technique. The results showed that 0.11--0.13 ng/ml of digoxin bound per milligram of fibrils and could not be separated by repeated washings. Elution with citrate or changes in the pH of the buffer. Immunofluorescent studies demonstrated diffusely bright immunofluorescence with the fibril preparation and amyloid heart homogenate when reacted with digoxin and digoxin-specific antiserum. These studies demonstrate that isolated amyloid fibrils bind digoxin and suggest that this interaction may play some role in the sensitivity to digitalis that has been observed in some patients with amyloid cardiomyopathy.
D000682:Amyloid / Q000378:metabolism; D000818:Animals; D000906:Antibodies; D001665:Binding Sites; D009202:Cardiomyopathies / Q000378:metabolism*; D004077:Digoxin / Q000276:immunology / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D006321:Heart; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D009132:Muscles; D011817:Rabbits
D000682:Amyloid; D000906:Antibodies; D007106:Immune Sera; D004077:Digoxin