[Investigations for improving the microscopic detection of mycobacteria following rapid cultivation by means of a rotating incubator. I. Improvement of the fixation technique (author's transl)].
The optimal qualitative and quantitative identification by microscopy of mycobacteria cultivated in liquid medium in including several problems. Acridine-orange was used for staining in all investigations. Polyvinyl-alcohol (1--2.5%) proved to be the best means for fixing mycobacteria culture suspensions on slides. A reliable sterilization of the sides was possible with peracetic acid (3%). Tween 80 in the culture medium did not impair the adherence of the fixed suspension on the slide under these conditions. Mycobacteria in a suspension of 10(-4) mg (wet weight) per ml could be still found by microscopy with this procedure.
D000165:Acridine Orange; D001431:Bacteriological Techniques; D003470:Culture Media; D005404:Fixatives; D008853:Microscopy / Q000379:methods*; D009161:Mycobacterium; D011142:Polyvinyl Alcohol
D003470:Culture Media; D005404:Fixatives; D011142:Polyvinyl Alcohol; D000165:Acridine Orange
Untersuchungen zur Optimierung des mikroskopischen Nachweises von Mykobakterien nach Schnellanzucht im Rotationsinkubator. I. Mitteilung: Verbesserung der Fixationstechnik.