Induction of rho- mutations in yeast Saccharomyces cerevisiae by ethanol.
The kinetics of the killing effect of ethanol was studied at 6-30% concentrations. Ploidy of cells, deficiency of the excision-repair system or holding under no-growth conditions did not influence survival. Ethanol at 24% increased, in the wild strain, the number of respiration-deficient cells from a spontaneous level of 0.4% up to nearly half of all survivors. Genetic analysis showed the mitochondrial nature of induced respiration-deficient mutants (or rho-). The influence of yeast resistance to some antibiotics was studied on rho- mutagenesis, both spontaneous and induced by ethanol. Neomycin-resistant strains were characterized by a significantly lower level of these mutations than were neomycin-sensitive strains.
D004260:DNA Repair; D004305:Dose-Response Relationship, Drug; D004352:Drug Resistance, Microbial; D000431:Ethanol / Q000494:pharmacology*; D006238:Haploidy; D007700:Kinetics; D008928:Mitochondria / Q000187:drug effects*; D009154:Mutation; D010085:Oxidative Phosphorylation; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*
D000431:Ethanol