Applications of fluorophore-containing microbial growth media.
Media containing the fluorogenic compound 8-anilino-1-naphthalene sulfonic acid may be used to discriminate between gram-positive and gram-negative bacteria and to differentiate between various species of bacteria. Fluorescent light emitted from colonies of gram-negative bacteria on 8-anilino-1-naphthalene sulfonic acid-containing agar was visually more intense than that on gram-positive bacteria. The emitted light from the gram-negative bacteria differed in wave-lengths from that of light emitted by colonies of gram-positive bacteria. The fluorescent intensity of colonies on complete 8-anilino-1-napthalene sulfonic acid agar supplemented with 1% of single substrates varied depending on the bacterial species, thus allowing the development of profiles used to identify 12 different species.
D000817:Anilino Naphthalenesulfonates / Q000494:pharmacology*; D001419:Bacteria / Q000145:classification*; D001431:Bacteriological Techniques; D002473:Cell Wall; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects; D005453:Fluorescence; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000187:drug effects
D000817:Anilino Naphthalenesulfonates; D003470:Culture Media