Possible role of colonic content in the mucosal association of pathogenic shigella.
Association of Shigella flexneri to cecal membrances was studied by incubating the pathogen with cecal slices of germfree mice. The slices were first incubated with stool supernatants from germfree, shigella-monocontaminated, and conventional animals. Quantitation of shigellae in homogenates of treated slices revealed an increase of organisms only in those slices exposed to contaminated stool supernatants.
D000818:Animals; D002021:Buffers; D002432:Cecum / Q000382:microbiology / Q000473:pathology; D003106:Colon / Q000382:microbiology*; D005856:Germ-Free Life; D007413:Intestinal Mucosa / Q000382:microbiology*; D051379:Mice; D012763:Shigella flexneri / Q000472:pathogenicity*
D002021:Buffers