Mass-spectrometric evidence for spontaneous and angiotensin-induced generation of prostacyclin by perfused cat lungs.
Isolated perfused cat lungs secreted spontaneously a PGI2-like substance that relaxed a strip of bovine coronary artery. The presence of PGI2 was confirmed by the identification of 6-oxo-PGF1 alpha by GC-MS. Both bioassay and mass fragmentography showed that PGI2 was released at a rate of 4 - 12 ng/ml. Generation of PGI2 by the perfused cat lungs was stimulated by angiotensin II (3 - 10 ng/ml). The above results along with our in vivo experiments point to the lung as an important source of endogenous PGI2 in the body.
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001011:Aorta / Q000187:drug effects; D001681:Biological Assay; D002415:Cats; D002417:Cattle; D003331:Coronary Vessels / Q000187:drug effects; D011464:Epoprostenol / Q000096:biosynthesis* / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D010477:Perfusion; D011453:Prostaglandins / Q000096:biosynthesis*; D011817:Rabbits
D011453:Prostaglandins; D000804:Angiotensin II; D011464:Epoprostenol