Studies of ribosomal proteins of yeast species and their hybrids: gel electrophoresis and immunochemical cross-reactions.
The cytoplasmic ribosomal proteins (r-proteins) of seventeen yeast species of the genera Saccharomyces and Kluyveromyces were analyzed by one-dimensional gradient polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. The electrophoretic patterns of cytoplasmic r-proteins from different species display extensive differences in both the 40S and the 60S subunit. Relatedness of species suggested by r-protein patterns correlates well with that based on DNA/DNA homology (Bicknell and Douglas 1970). Immunochemical cross-reactions and antibiotic susceptibility tests were also used to compare different species. Analyses of r-proteins from two different interspecific hybrids showed that their ribosomes were hybrid, containing r-proteins from both parents. These findings are discussed in relation to the evolution of yeast species and the regulation of expression of r-proteins in eucaryotes.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001203:Ascomycota / Q000378:metabolism; D003429:Cross Reactions; D004352:Drug Resistance, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000276:immunology / Q000378:metabolism*; D006824:Hybridization, Genetic; D012269:Ribosomal Proteins / Q000276:immunology / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013045:Species Specificity; D015003:Yeasts / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D005656:Fungal Proteins; D012269:Ribosomal Proteins