Cell types in long-term propagable cultures of rat liver.
Under the conditions of this experiment, propagable hepatic epithelial cells in long-term culture were derived from a small, nonparenchymal precursor cell present in the liver in vivo. Progeny of the nonparenchymal precursor cell weakly expressed some hepatocyte-like properties in culture. Hepatocytes were not clonogenic under the conditions of this experiment. Propagable hepatic epithelial cells in long-term culture probably originate from hepatic stem cells in vivo. Terminal biliary ductular cells are facultative stem cells for hepatocytes.
D000818:Animals; D001653:Bile Ducts, Intrahepatic / Q000166:cytology; D002454:Cell Differentiation; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D002999:Clone Cells; D003593:Cytoplasm / Q000648:ultrastructure; D004847:Epithelial Cells; D005260:Female; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051381:Rats; D014357:Trypsin / Q000494:pharmacology
D014357:Trypsin