Partition affinity ligand assay (PALA). A new approach to binding assays.
A two-phase partitioning technique was used to separate free from bound labelled ligand in a binding assay. Efficient separation was accomplished by modifying one of the reactants in such a way that it partitions preferentially to one of the two phases. The biospecific interactions between the lectin concanavalin A and various carbohydrates and glycoproteins were investigated in this model study.
D000937:Antigen-Antibody Reactions; D001665:Binding Sites; D001667:Binding, Competitive; D003208:Concanavalin A / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006735:Horseradish Peroxidase / Q000494:pharmacology; D007158:Immunologic Techniques; D008358:Mannose / Q000494:pharmacology; D009005:Monosaccharides; D011134:Polysaccharides
D009005:Monosaccharides; D011134:Polysaccharides; D003208:Concanavalin A; D006735:Horseradish Peroxidase; D005947:Glucose; D008358:Mannose