The importance of the range of cooling rates within a Nagington and Greaves plug when freezing lymphocytes.
The use of Nagington and Greaves plug for freezing lymphocytes is very straight-forward but the recovery of cells can be low and variable. The survival of lymphocytes was found to be dependent upon the position of the sample within the plug and the plug position in the lipid nitrogen container. Both of these variables affected the rate of cooling of the samples. It is stressed that the range of cooling rates produced by a plug should be determined to ensure that lymphocytes are cooled at suitable rates.
D002470:Cell Survival; D003080:Cold Temperature; D005615:Freezing; D006801:Humans; D007158:Immunologic Techniques; D008214:Lymphocytes