The regeneration of epidermal cells of Saintpaulia leaves as a new plant-tissue system for cellular radiation biology.
Investigation of the nucleus of epidermal cells of the petioles of Saintpaulia leaves by cytofluorimetry revealed that all cells are in a non-cycling pre DNA synthesis phase. Cultivation of dissected leaves results in a synchronous regeneration process of a defined number of cells. Five days after onset of cultivation the cells reach the first mitosis. The nuclear development during the regeneration process is described. Irradiation of the leaves results in a directly visible inhibition of this regenerating capability which is used to quantify cell survival in a tissue. The data show that the radiation response has a similar shape to that of the survival of single cells in culture. This response can be observed before the first mitosis of the cells and its application as a new plant tissue system for cellular radiation research is discussed.
D002453:Cell Cycle / Q000528:radiation effects; D003584:Cytological Techniques; D008938:Mitosis / Q000528:radiation effects; D010944:Plants / Q000528:radiation effects*; D012038:Regeneration / Q000187:drug effects