Gas chromatographic determination of extracellular metabolites produced by baker's yeast during glucose-induced acidification.
Gas chromatographic separation of metabolites extruded during the medium acidification by resting baker's yeast supplied with glucose is described. Silylation with bis(trimethylsilyl)trifluoroacetamide and trimethylchlorosilane and a direct chromatography on Chromosorb G-AW-DMCS with 2.5% SE-52 (lactic, pyruvic, succinic, glyceric, fumaric, malic), polyols (glycerol, arabinitol) and sugars (glucose). The yeast was found to extrude glycerol, lactic, malic, and succinic acid.
D002849:Chromatography, Gas / Q000379:methods*; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008293:Malates / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism
D007773:Lactates; D008293:Malates; D013386:Succinates; D005947:Glucose; D005990:Glycerol