Cytochrome b-565 in Saccharomyces cerevisiae: use of mutants in the cob-box region of the mitochondrial DNA to study the functional role of this spectral species of cytochrome b. 1. Measurements of cytochromes b-562 and b-565 and selection of revertants devoid of cytochrome b-565.
In order to study the functional role of the spectral species of cytochrome b-565 observed in mitochondria, genetically manipulated strains of Saccharomyces cerevisiae have been used. Strains have been found which are devoid of cytochrome b-565 under certain conditions and which nevertheless are able to grow on a respirable substrate. Two different methods have been used to determine the cytochrome b-565 content: anaerobic titrations and antimycin-A-induced reduction of cytochrome b-565. Both yield the same results.
D000332:Aerobiosis; D000693:Anaerobiosis; D000968:Antimycin A; D003573:Cytochrome b Group; D003580:Cytochromes / Q000378:metabolism*; D004272:DNA, Mitochondrial / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005838:Genotype; D009154:Mutation; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013045:Species Specificity; D013053:Spectrophotometry; D013696:Temperature
D003573:Cytochrome b Group; D003580:Cytochromes; D004272:DNA, Mitochondrial; D029968:Escherichia coli Proteins; D000968:Antimycin A; C017850:cytochrome b562, E coli; C027737:cytochrome b565