Kinetic ethylene glycol assay with use of yeast alcohol dehydrogenase.
We describe a rapid, accurate, and precise two-point kinetic assay for ethylene glycol. The method involves use of a standard kit for ethanol determination with yeast alcohol dehydrogenase, and of a centrifugal analyzer. Alcohol dehydrogenase catalyzes the oxidation of ethylene glycol in a trichloroacetic acid-precipitated specimen; the resulting NADH production is monitored at 340 nm. The reaction rate is linear with concentration to 1.5 g of ethylene glycol per liter. Interference from methanol, ethanol, and isopropanol was easily recognized after a 30-min incubation at 100 degrees C. We believe that the method can be readily adaptable to any narrow-bandwidth, stable, temperature-controlled spectrophotometer and so should provide more widely for the prompt assessment of patients in whom ethylene glycol poisoning is suspected.
D000429:Alcohol Oxidoreductases; D001322:Autoanalysis; D005026:Ethylene Glycols / Q000097:blood*; D006801:Humans; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D005026:Ethylene Glycols; D000429:Alcohol Oxidoreductases