title: Requirement for protein synthesis for survival of unmodified bacteriophage T1 in a restricting host.
abstract: At high multiplication of infection, a substantial fraction of restricting cells (P1 lysogens) could be productively infected by unmodified coliphage T1 (T1.0) provided that protein synthesis was uninhibited during the first 5 min of infection. Successful infection under restricting conditions was accompanied by more genetic recombination than was seen under nonrestricting host, the recombination frequency declined for markers on T1.0 genomes; no effect was seen on recombination between markers on modified (T1.P) genomes. This suggested that recombination between unmodified genomes may be essential for their survival under conditions of host restriction. In a restricting host, genetic markers on T1.0 could recombine with T1.P even when the rescuing phage was added 6 min after T1.0 infection. However, even marker rescue recombination was diminished when protein synthesis was inhibited during early infection. Since DNA restriction is an early event, protein synthesis may be required soon after infection of a restricting host by T1.0 in order to preserve restriction-damaged DNA in a form that can participate in recombination. Experiments are also described that rule out some possibilities for the role of such a protein(s).
mesh_terms: D002701:Chloramphenicol / Q000494:pharmacology; D004926:Escherichia coli / Q000502:physiology; D005814:Genes, Viral; D005819:Genetic Markers; D008242:Lysogeny; D011995:Recombination, Genetic; D013604:T-Phages / Q000235:genetics / Q000502:physiology*; D014764:Viral Proteins / Q000096:biosynthesis*
chemical_list: D005819:Genetic Markers; D014764:Viral Proteins; D002701:Chloramphenicol