[Study of a kit for the search for HBs antigen by an enzymo-immunological method (author's transl)].
The authors present a kit marketed by Abbott for searching HBs antigen by an immunological method. This technic of sandwich type included 3 stages. During a first stage, the HBs antigen eventually present was extracted from the biological fluid by a specific antibody adsorbed on a polystyrène bead. After washing, a second specific antibody labelled with peroxidase was added. After incubation and washing, the peroxidase activity of the bead was revealed by adding a chromogenic substrate. The development of a colour indicated the presence of HBs antigen in the biological fluid under study. Very simple to use, this kit permits specific detection of HBs antigen with a sensitivity comparable to that of radio-immunoassay. A good correlation was obtained with the radio-immunological kit Austria II and with the enzymo-immunological kit Hepanostika.
D000918:Antibody Specificity; D006514:Hepatitis B Surface Antigens / Q000302:isolation & purification*; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D010544:Peroxidases; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic
D006514:Hepatitis B Surface Antigens; D011933:Reagent Kits, Diagnostic; D010544:Peroxidases
Etude d'une trousse pour la recherche de l'antigène HBs par méthode enzymo-immunologique.