Improved cell surface radioiodination of macrophages.
Rabbit alveolar macrophages at high cell concentrations are inefficiently radiolabeled by the usual lactoperoxidase-catalyzed iodination method. Soluble macromolecules secreted by macrophages were found to inhibit the radioiodination of macrophages and other cells. A modified procedure is described which minimizes the presence of such inhibitory material and thereby considerably improves the radioiodination efficiency for both alveolar macrophages and the macrophage-like P388D1 cell line.
D000818:Animals; D001491:Basophils / Q000276:immunology; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology*; D007158:Immunologic Techniques; D007457:Iodine Radioisotopes; D007784:Lactoperoxidase; D007941:Leukemia P388 / Q000276:immunology; D007942:Leukemia, Experimental / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D011650:Pulmonary Alveoli / Q000276:immunology; D011817:Rabbits; D051381:Rats
D007457:Iodine Radioisotopes; D007784:Lactoperoxidase