Insulin-induced renin release: blockade by indomethacin in the rat.
1. Since prostaglandins appear to mediate adrenergically stimulated renin release, the effect of indomethacin was examined on insulin-induced renin and catecholamine release in conscious rats. Insulin (10 units/kg subcutaneously) increased plasma renin activity from 2.8 +/- 0.5 to 9.0 +/- 1.1 pmol h-1 ml-1 (P less than 0.001) while also increasing plasma adrenaline, noradrenaline and the urinary excretion of prostaglandin E2 and F2 alpha. Plasma potassium and glucose were reduced by 16 and 54% respectively. 2. Indomethacin (14 mumol/kg subcutaneously) reduced the urinary excretion of prostaglandin E2 and F2 alpha by 67 and 54% respectively, without altering the other parameters. 3. Indomethacin inhibited insulin-induced renin release by 67% (P less than 0.02) and blocked the insulin-induced increases in urinary prostaglandin E2 and F2 alpha. The insulin-induced changes in plasma catecholamines, potassium and glucose were unaltered by indomethacin. 4. These findings suggest that renal prostaglandins mediate this form of adrenergically stimulated renin release by acting at a side distal to the beta-adrenoreceptor.
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D004837:Epinephrine / Q000097:blood; D007213:Indomethacin / Q000378:metabolism / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000097:blood; D011188:Potassium / Q000097:blood; D011458:Prostaglandins E / Q000652:urine; D011460:Prostaglandins F / Q000652:urine; D051381:Rats; D012083:Renin / Q000097:blood*; D000092502:Renin Inhibitors
D001786:Blood Glucose; D004837:Epinephrine; D007213:Indomethacin; D007328:Insulin; D009638:Norepinephrine; D011188:Potassium; D011458:Prostaglandins E; D011460:Prostaglandins F; D012083:Renin; D000092502:Renin Inhibitors