Separation and quantification of angiotensins and some related peptides by high-performance liquid chromatography.
A high-performance chromatographic technique for the separation of angiotensins and some related peptides is described. Complete separation of angiotensin I, angiotensin II, tetradecapeptide and the tetrapeptide Leu-Val-Tyr-Ser is achieved in a single step, using reversed-phase high-performance liquid chromatography. The application of this technique for the detection of renin activity in crude biological samples, employing the artificial renin substrate tetradecapeptide, is demonstrated.
D000803:Angiotensin I / Q000032:analysis* / Q000378:metabolism; D000804:Angiotensin II / Q000032:analysis*; D000808:Angiotensinogen / Q000032:analysis*; D000809:Angiotensins / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D009842:Oligopeptides / Q000032:analysis*; D010455:Peptides / Q000378:metabolism; D051381:Rats; D012083:Renin / Q000378:metabolism
D000809:Angiotensins; D009842:Oligopeptides; D010455:Peptides; D000808:Angiotensinogen; D000804:Angiotensin II; D000803:Angiotensin I; D012083:Renin