Albumin deposition in dermal capillary basement membrane in insulin-dependent diabetes mellitus. A preliminary report.
Skin specimens obtained from 24 insulin-dependent diabetics (IDD) and 35 controls were examined by immunofluorescence (IF) microscopy. An increased percentage (greater than 8%) of dermal vessel basement membranes (BM) showing circumferential fluorescence for albumin was observed in 18 of 24 IDD (duration of diabetes 1.5-33 yr) and 1 of 35 controls. Dual label studies using fluorescein-conjugated anti-human albumin and rhodamine-conjugated anti-human BM confirmed that localization of albumin was within the BM. These studies suggest that increased albumin localization in extracellular BM may be widespread in diabetes.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000418:Albumins / Q000378:metabolism*; D001485:Basement Membrane / Q000378:metabolism; D002196:Capillaries; D002648:Child; D003920:Diabetes Mellitus / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D006455:Hemoglobins, Abnormal / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000378:metabolism*
D000418:Albumins; D006455:Hemoglobins, Abnormal