title: Angiotensin and single nephron glomerular function in the trout Salmo gairdneri.
abstract: Glomerular filtration rate (GFR), urine flow (UV), renal tubular transport maximum for glucose (TMG), and single nephron glomerular filtration rate (SNGFR), determined in anesthetized norepinephrine-infused trout (Salmo gairdneri), were 18.51 +/- 5.78 microliter x min-1 x kg body wt-1, 5.31 +/- 1.38 microliter x min-1 x kg body wt-1, 105.21 +/- 46.84 microgram x min-1 x kg body wt-1, and 3.74 +/- 1.12 nl/min, respectively, when in seawater (SW) and 140.39 +/- 17.24, 76.38 +/- 10.41, 626.16 +/- 77.46, and 1.31 +/- 0.20 in freshwater (FW). Angiotensin II infusions, to reduce UV, GFR, and TMG by 50%, had no effect on the average SNGFR of FW trout, but reduced that of SW fish to 1.42 +/- 0.19 nl/min. Infusions of 20% ferrocyanide, visualized as Prussian blue (PB), identified three glomerular populations: filtering (F) with PB in glomerular vessels and tubular lumen; nonfiltering (NF)--PB in glomerular vessels only; nonperfused (NP)--no PB associated with the nephron. SW and FW kidneys contained about 40% NF tubules. In FW, 45% were F tubules compared with 5% in SW, whereas NP tubules comprised 51% of SW tubules and 13% of FW. During angiotensin II infusions the distributions were 9% and 46% NF in FW and 6% F and 12% NF in SW trout.
mesh_terms: D000804:Angiotensin II / Q000502:physiology*; D000818:Animals; D004231:Diuresis; D005618:Fresh Water; D005919:Glomerular Filtration Rate / Q000187:drug effects; D007668:Kidney / Q000502:physiology*; D007684:Kidney Tubules / Q000378:metabolism; D009638:Norepinephrine / Q000494:pharmacology; D012083:Renin / Q000097:blood; D012474:Salmon / Q000502:physiology*; D012623:Seawater
chemical_list: D000804:Angiotensin II; D012083:Renin; D009638:Norepinephrine