[ATP-insulin conjugates and their use for immunofactor analysis].
A method resulting in ATP-insulin conjugates by covalent binding of ATP modified at C(6) amino group of the adenine residue with insulin was developed. The modified ATP was bound to insulin by means of metha-p-toluene sulfonate-N-cyclohezyl Nf [2-morpholinyl(4)ethyl]-carbodiimide. The ATP analogs and ATP-insulin conjugates possess the coenzyme activity in a reaction of luciferin oxidation by luciferase from the fireflies Luciola mingrelica. the catalytic properties of soluble and immobilize on CNBR-activated. Sepharose enzymes in reactions with native ATR, its modified derivatives and ATP--insulin conjugates were compared. The bioluminescence reaction involving ATP--insulin conjugate is inhibited by antibodies against insulin. This effect can form a basis for insulin detection in solution, which is based on competitive binding of free and antibody-labelled ATP--insulin conjugates.
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D003028:CME-Carbodiimide / Q000031:analogs & derivatives; D001517:Coleoptera / Q000201:enzymology; D004800:Enzymes, Immobilized / Q000378:metabolism; D007328:Insulin / Q000032:analysis; D007330:Insulin Antibodies; D007700:Kinetics; D008156:Luciferases / Q000378:metabolism; D008163:Luminescent Measurements
C029524:ATP-insulin conjugates; D004800:Enzymes, Immobilized; D007328:Insulin; D007330:Insulin Antibodies; D003028:CME-Carbodiimide; D000255:Adenosine Triphosphate; D008156:Luciferases
Kon"iugaty ATP--insulin i ikh ispol'zovanie dlia immunokofaktornogo analiza.