title: Hapten-specific T cell responses to 4-hydroxy-3-nitrophenyl acetyl. VII. Idiotype-specific suppression of plaque-forming cell responses.
abstract: The ability of suppressor cells induced by the intravenous administration of 4-hydro-3-nitrophenyl acetyl (NP)-modified syngeneic cells to reduce an idiotypic B cell response was studied in both an in vivo and an in vitro system. Idiotype-positive B cells were assayed by the ability of guinea pig anti-idiotypic antiserum to specifically inhibit idiotype-positive plaque formation. It was found that up to 57% of the PFC response in vivo and 100% of the PFC response in vitro was inhibitable with antiidiotypic antiserum. The expression of these idiotype-positive B cells could be suppressed by the transfer of spleen cells form mice treated 7 d previously with NP coupled syngeneic cels. T cells are both required and sufficient for the transfer of idiotype specific suppression. The induction of these idiotype-specific T suppressor cells directly with antigen suggests that recognition of unique determinants on cell surfaces is important for regulation of lymphoid cell interactions. The role of idiotype-specific suppressor cells in the network of lymphoid interactions is discussed.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000276:immunology; D005260:Female; D005362:Ficoll / Q000276:immunology; D006168:Guinea Pigs; D006241:Haptens; D006462:Hemolytic Plaque Technique; D007106:Immune Sera / Q000494:pharmacology; D007130:Immunoglobulin Idiotypes; D007165:Immunosuppression Therapy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008819:Mice, Nude; D009596:Nitrophenols / Q000276:immunology*; D010455:Peptides / Q000276:immunology; D010648:Phenylacetates; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D006241:Haptens; D007106:Immune Sera; D007130:Immunoglobulin Idiotypes; D009596:Nitrophenols; D010455:Peptides; D010648:Phenylacetates; C026242:4-hydroxy-5-nitrophenyl acetic acid; D005362:Ficoll