Antibodies to human immunoglobulin detected by hemolysis of human immunoglobulin-coated red blood cells.
A hemolysis assay was developed to detect alloantibodies to human immunoglobulin. A total of 1035 serum samples was tested. Anti-IgM antibodies were found in 8% of 59 normal persons and in 13% of 439 multiparous women, with the highest incidence of 67% in 341 dialysis patients. Although the anti-IgM antibodies were inhibited by both IgM and IgG, it appeared that they were also inhibited by F(ab')2 bu not by Fc. Anti-IgG antibodies were more strongly inhibited by Fc than F(ab')2. These results suggest that anti-IgM antibodies might be analogous to antiidiotypic antibodies directed to F(ab')2, whereas anti-IgG antibodies tend to have greater reactivity to Fc.
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis* / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006461:Hemolysis; D006801:Humans; D007126:Immunoglobulin Allotypes / Q000032:analysis / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D007158:Immunologic Techniques; D012217:Rheumatoid Factor / Q000032:analysis
D000888:Antibodies, Anti-Idiotypic; D007126:Immunoglobulin Allotypes; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012217:Rheumatoid Factor