In situ binding of AT-rich repetitive DNA to the centromeric heterochromatin in polytene chromosomes of chironomids.
Native highly repetitive DNA sequences have been allowed to react in situ with DNA-depleted polytene chromosomes of chironomids in cytological preparations. The double-stranded DNA can bind specifically to the centromeric heterochromatin, where these sequences have been localized previously by in situ hybridization. Various control experiments support the conception that heterochromatin-specific DNA-binding proteins are involved in the in situ binding.
D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000378:metabolism; D002503:Centromere / Q000648:ultrastructure*; D002683:Chironomidae / Q000648:ultrastructure; D002875:Chromosomes / Q000648:ultrastructure*; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins; D006570:Heterochromatin / Q000378:metabolism* / Q000648:ultrastructure; D006358:Hot Temperature; D010447:Peptide Hydrolases / Q000378:metabolism; D011485:Protein Binding; D012091:Repetitive Sequences, Nucleic Acid; D012260:Ribonucleases / Q000378:metabolism
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D006570:Heterochromatin; D004247:DNA; D012260:Ribonucleases; D010447:Peptide Hydrolases