[Entry of double-and single-stranded linear DNA in Ca2+-treated Escherichia coli cells].
Hydroxylapatite chromatography technique was used to investigate the conformational changes undergone by exogenous linear DNA during the uptake by Ca2+-treated EScherichia coli cells. Both single- (heat denatured) and double-stranded DNAs were examined. While native DNA preserved its double-strandedness during the uptake, the heat denatured (single-stranded) DNA was rapidly converted to the double-stranded form. This process was energy-dependent and is supposed to be dependent on gene recA product.
D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D002118:Calcium
Pogloshchenie lineĭnykh odno- i dvukhtsepochechnykh DNK kletkami Escherichia coli, obrabotannymi kationami Ca2+.