Comparison of isotopic equilibrium and dual isotope blood ratio methods for measurement of cholesterol absorption in rats.
The dual isotope plasma ratio method (IRM) was applied to eight 3-month-old rats fed a semipurified diet. 1 month later, the isotopic equilibrium method (IEM) was substituted and was continued for 2 months. The animals were therfore 6 months old at the end of the isotope experiment. The IRM was then applied to a second group of eight 6-month-old rats. No difference was seen between the absorption coefficient of cholesterol measured by the IRM in rats of the first (73 +/- 2%) and of the second groups (72 +/- 4%) or by the IEM (69 +/- 1%). A critical discussion of both techniques is presented.
D000818:Animals; D002250:Carbon Radioisotopes; D002784:Cholesterol / Q000008:administration & dosage / Q000378:metabolism*; D002791:Cholesterol, Dietary; D005243:Feces / Q000032:analysis; D007275:Injections, Intravenous; D007279:Injections, Subcutaneous; D007408:Intestinal Absorption; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008297:Male; D011865:Radioisotope Dilution Technique; D051381:Rats; D013261:Sterols / Q000032:analysis; D014316:Tritium
D002250:Carbon Radioisotopes; D002791:Cholesterol, Dietary; D013261:Sterols; D014316:Tritium; D002784:Cholesterol