Inhibition of mitogen-induced lymphokine production by cyclosporin A.
The new antilymphocytic agent Cyclosporin A was found to inhibit the production and/or secretion of migration inhibitory factor (MIF) in human lymphocytes stimulated by Concanavalin A. Preincubation for one hour with the compound, followed by 8 hr restoration period of the cells in absence of the drug, resulted in moderate decrease in MIF synthesis and/or release. Cell viability was not affected. The agent was shown not to interfere with MIF action on the macrophage. We conclude that the molecular mechanism of action of Cyclosporin A is based, at least partially, on a blockade of synthesis and/or secretion of lymphokines from immunocompetent cells.
D003208:Concanavalin A / Q000494:pharmacology; D003524:Cyclosporins; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology; D008214:Lymphocytes / Q000187:drug effects; D008222:Lymphokines / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D008263:Macrophage Migration-Inhibitory Factors / Q000037:antagonists & inhibitors*; D010456:Peptides, Cyclic / Q000494:pharmacology*
D003524:Cyclosporins; D008222:Lymphokines; D008263:Macrophage Migration-Inhibitory Factors; D010456:Peptides, Cyclic; D003208:Concanavalin A