Simple filter paper procedure for estimation of glucose uptake via group translocation by whole-cell suspensions of bacteria.
A simple and rapid filter paper technique is described for processing samples from glucose uptake studies with whole cells of gram-positive or gram-negative bacteria that transport glucose via group translocation. The procedure yields results equivalent to those obtained with a conventional membrane filtration method and requires no special filtration equipment or source of vacuum.
D001431:Bacteriological Techniques; D001692:Biological Transport; D003847:Deoxyglucose / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005374:Filtration / Q000379:methods; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D013295:Streptococcus mutans / Q000378:metabolism*
D003847:Deoxyglucose; D005947:Glucose