Effect of cryo-protective agents on the growth and ultrastructure of Saccharomyces cerevisiae.
When suspended in a glucose medium supplemented with varying amounts of DMSO, ethylene glycol, glycerol, lactose and sodium chloride at its optimum temperature of 30 degrees C, a diploid strain of Saccharomyces cerevisiae gave a mean generation time inversely proportional to the increased concentrations of these anti-freeze compounds. No growth was attained at 0 degrees C and -5 degrees C, and mixtures of the compounds did not support any growth even at 30 degrees C. At 0 degrees C and -5 degrees C with increasing percentages of the individual compounds, and at 30 degrees C, 0 degrees C and -5 degrees C, with mixtures of the compounds, a significant decrease in survival was obtained. As compared to cells grown in the absence of antifreeze compounds, electron microscopy of the cells which grew at 30 degrees C in the presence of antifreeze compounds showed thicker cell walls, highly convoluted plasmalemma, vacuoles filled with electron-dense fibrous material, spherosomes, poorly developed mitochondria and many vesicles.
D002462:Cell Membrane / Q000648:ultrastructure; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005026:Ethylene Glycols / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D007785:Lactose / Q000494:pharmacology; D009940:Organoids / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D012965:Sodium Chloride / Q000494:pharmacology
D003451:Cryoprotective Agents; D005026:Ethylene Glycols; D012965:Sodium Chloride; D007785:Lactose; D005990:Glycerol; D004121:Dimethyl Sulfoxide