Universal reagent immunosorbent assay (URI) for haptens.
Preliminary data on the use of a universal reagent, 125I-labelled second antibody, for the measurement of small molecules using immunosorbent techniques are described. The results showed that for two chemically unrelated haptens, norethisterone and diphenoxylic acid, the procedure could be optimised to measure these molecules at concentrations similar to those measurable using conventional liquid-phase or solid-phase techniques. No significant loss of norethisterone-antibody specificity could be demonstrated.
D000818:Animals; D006241:Haptens / Q000276:immunology / Q000302:isolation & purification; D007163:Immunosorbent Techniques; D009640:Norethindrone / Q000032:analysis; D011817:Rabbits / Q000276:immunology
D006241:Haptens; D009640:Norethindrone