[Detection of hepatitis Be antigen with an enzyme immunoassay (author's transl)].
An enzyme immunoassay (ELISA) from SEROMED was tested for its suitability in detecting HBeAg and was compared with a radioimmunoassay (RIA) from ABBOTT and with rheophoresis. From a total of 931 sera tested, HBeAg could be detected in 313 sera with ELISA, 347 sera with RIA and 22 sera with rheophoresis. When 8 HBeAg containing sera were titrated for end points, RIA gave titers which were 2.3-fold higherr than ELISA and 575-fold higher than rheophoresis. The differences between RIA and ELISA were shown to be due to somwhat lower sensitivity of ELISA. However, when sera from patients with Hepatitis B from various times after the onset of hepatitis (maximally 12 months) were tested, there was only a slight difference in sensitivity between ELISA and RIA. ELISA along with RIA is considered to be a substantially sensitive test for detection of HBeAG as compared to rheophoresis and can be recommended for routine use.
D001782:Blood Donors; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D006509:Hepatitis B / Q000276:immunology; D006511:Hepatitis B Antigens / Q000032:analysis*; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006513:Hepatitis B e Antigens / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D008107:Liver Diseases / Q000276:immunology; D011863:Radioimmunoassay; D019966:Substance-Related Disorders / Q000276:immunology
D006511:Hepatitis B Antigens; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens
Der Nachweis von Hepatitis Be-Antigen mit einem Enzymimmunoassay.