Complementation of the plaque-forming cell responses of T-cell-deficient nude mice by a T-cell hybridoma.
Spleen cells from NFR/N mice primed with carrier rabbit erythrocytes were passed through nylon wool and fused with cells from the AKR T-cell lymphoma BW5147 in order to construct functional T-cell hybridomas. Several hybrids have been obtained. One of the cloned hybrids possesses a marked capacity to functionally complement the T-cell-dependent hapten-specific plaque-forming cell responses of NFR/N nude mice to the trinitrophenyl group. The hybrids carry the Thy 1 marker of the immune parent, express the Lyt 1 surface marker, possess Fc receptors at their surface, and express an Ig heavy-chain variable region determinant at their surfaces as detected by anti-VH MOPC 315 antiserum.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D005816:Genetic Complementation Test; D006462:Hemolytic Plaque Technique; D006822:Hybrid Cells / Q000276:immunology*; D007621:Karyotyping; D008223:Lymphoma; D051379:Mice; D008819:Mice, Nude; D010954:Plasmacytoma; D011961:Receptors, Fc / Q000032:analysis; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D011961:Receptors, Fc