title: Temperature-sensitive glutamate dehydrogenase mutants of Salmonella typhimurium.
abstract: Mutants of Salmonella typhimurium defective in glutamate dehydrogenase activity were isolated in parent strains lacking glutamate synthase activity by localizcd mutagenesis or by a general mutagenesis combined with a cycloserine enrichment for glutamate auxotrophs. Two mutants with temperature-sensitive phenotypes had glutamate dehydrogenase activities that were more thermolabile than that of an isogenic control strain. Eight other mutants had less than 10% of the wild-type glutamate dehydrogenase activity. All the mutations were cotransducible with a Tn10 element (zed-2:Tn10) located at approximately 23 U on the S. typhimurium linkage map. These data strongly indicate that this region contains the structural gene (gdhA) for glutamate dehydrogenase.
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D005796:Genes; D005969:Glutamate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D011188:Potassium / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000235:genetics*; D013696:Temperature
chemical_list: D005969:Glutamate Dehydrogenase; D011188:Potassium