Influence of culture conditions on B-cell functions in vitro.
Isolated pancreatic islets from Wistar rats were maintained in vitro at 37 degrees C for 12 days in TCM 199 containing 10 mmol/l glucose and supplemented with 10% serum. 10 mmol/l glucose completely prevented the diminution of insulin content independent of the type of serum (fetal calf serum; serum from newborn calfs; horse serum), whereas the insulin biosynthesis as well as the insulin release were modulated by the kind of serum. Fetal calf serum can be replaced by horse serum.
D000818:Animals; D002478:Cells, Cultured; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000096:biosynthesis / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000502:physiology*; D011384:Proinsulin / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D011384:Proinsulin; D005947:Glucose