Prolonged bone marrow and skin allograft survival after pretransplant conditioning with cyclophosphamide and total lymphoid irradiation.
Current studies were designed to provide long-term survival of allogeneic skin and bone marrow in mice preconditioned with various combinations of cyclophosphamide (CY) and/or total lymphoid irradiation (TLI). Long-term skin graft and bone marrow survival was obtained across the major histocompatibility barrier (BALB/c into C57BL/6) using pregrafting conditioning with either fractionated TLI or the combination of CY with a single dose of TLI. CY alone and a single dose of TLI alone were relatively ineffective as pregrafting immunosuppressive combinations. Allogeneic bone marrow was required for long-term skin graft survival with either conditioning regimen. Allogeneic marrow transplantation resulted in somewhat more deaths than syngeneic transplantation with both CY + TLI and fractionated TLI.
D000818:Animals; D016026:Bone Marrow Transplantation; D002678:Chimera / Q000528:radiation effects; D003520:Cyclophosphamide / Q000494:pharmacology*; D006085:Graft Survival / Q000187:drug effects / Q000528:radiation effects; D008221:Lymphoid Tissue / Q000276:immunology / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011829:Radiation Dosage; D016038:Skin Transplantation; D014184:Transplantation, Homologous
D003520:Cyclophosphamide