Kidney storage at subzero temperatures using a hyperosmolar perfusate.
32 dog kidneys were stored in a hyperosmolar perfusate (770 mosm/kg) at minus2 and plus4 degrees C, respectively, for 12-48 h and thereafter autotransplanted. 18 kidneys of the control group were stored in Collins solution at plus4 degrees C. The immediate function of the kidneys after transplantation was measured by p-aminohippuric acid and inulin clearances. Successful 24-hour preservation was possible by use of the hyperosmolar perfusate. Kidneys stored in the hyperosmolar perfusate at minus2 degrees C were better preserved than kidneys stored in Collins solution at plus4 degrees C. The addition of human albumin to the hyperosmolar perfusate did not improve the preservation results.
D000418:Albumins / Q000494:pharmacology; D000818:Animals; D004285:Dogs; D006085:Graft Survival / Q000187:drug effects; D007036:Hypothermia, Induced; D016030:Kidney Transplantation; D009926:Organ Preservation; D009994:Osmolar Concentration; D011188:Potassium / Q000494:pharmacology*; D014021:Tissue Preservation
D000418:Albumins; D011188:Potassium