Substrate specificity of lysophospholipase-transacylase from rat lung and its action on various physical forms of lysophosphatidylcholine.
Lysophospholipase-transacylase (lysolecithin acylhydrolase, EC 3.1.1.5) from rat lung catalyzes the transfer of acyl groups from lysophosphatidylcholine to either water or another molecule of lysophosphatidylcholine. Studies on the substrate specificity of the purified enzyme showed that a phosphate group in the substrate is essential for enzymatic activity; monoacylglycerol is not hydrolyzed, nor does it serve as an acceptor of acyl groups. The influence of the acyl chain in lysophosphatidylcholine was investigated by using mixtures of differently labelled lysophosphatidylcholine species, or by studying the transfer of [1-14C]Palmitate from [1-14C]palmitoylpropane (1,3)diol-phosphocholine to various 1-acyl-sn-glycero-3-phosphocholines. Lysophosphatidylcholines with acyl chains comprised of ten or more C-atoms were found to serve as acyl acceptors. This finding was used to determine the action of the enzyme on 1-[1-14C]auroyl- and 1[1-14C]myristoyl-sn-glycero-3-phosphocholine both below and above the critical micelle concentration of the substrate. Monomeric substrate was effectively hydrolyzed, but the transacylase activity of the enzyme was only expressed when substrate micelles were present. Likewise, no transacylase activity was found when lysophosphatidylcholine was embedded in liposomal membranes prepared from lung total lipids. These findings, which persist with crude enzyme preparations (100 000 x g supernatant), are discussed in relation to the putative function of the lysophospholipase-transacylase in the synthesis of disaturated phosphatidylcholine in lung.
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D007700:Kinetics; D008081:Liposomes; D008168:Lung / Q000201:enzymology*; D008244:Lysophosphatidylcholines; D008245:Lysophospholipase / Q000378:metabolism*; D010169:Palmitic Acids; D010740:Phospholipases / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D051381:Rats; D013379:Substrate Specificity
D002250:Carbon Radioisotopes; D008081:Liposomes; D008244:Lysophosphatidylcholines; D010169:Palmitic Acids; D000217:Acyltransferases; D010740:Phospholipases; D008245:Lysophospholipase