title: Serodiagnosis of human leptospirosis by enzyme-linked-immunosorrbent-assay (ELISA).
abstract: An enzyme-linked-immunosorbent-assay (ELISA) is described for the serodiagnosis of leptospirosis. Using an antigen prepared from a heated culture of a single leptospira strain (Wijnberg) the ELISA is a genusspecific test. The microscopic agglutination test (MAT) served as a reference. ELISA and MAT results agreed in 95% of the sera from 96 leptospirosis patients. One false positive was found in 217 controls. The ELISA is sensitive, specific and relatively easy to perform.
mesh_terms: D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D007922:Leptospirosis / Q000175:diagnosis*
chemical_list: D000907:Antibodies, Bacterial