Development of proteolytic activity in rabbit fetal intestine.
The development of proteolytic activity was determined in rabbit fetal intestine using 125I-hemoglobin as substrate. This assay method allowed increased sensitivity and complete linearity with incubation time and amount of homogenate protein. The effect of pH on substrate hydrolysis showed two main peaks of activity around pH 5 and 8. The apparent Km of proteolytic activity for the 125I-hemoglobin substrate was 0.57 mg/ml at pH 8. Proteolytic activity increases about 10-fold in fetal rabbit intestine between 21 and 31 days of gestation.
D000818:Animals; D003550:Cystic Fibrosis / Q000209:etiology; D006441:Hemoglobin A / Q000378:metabolism*; D007422:Intestines / Q000196:embryology / Q000201:enzymology*; D007457:Iodine Radioisotopes; D010447:Peptide Hydrolases / Q000378:metabolism*; D011480:Protease Inhibitors / Q000378:metabolism; D011817:Rabbits
D007457:Iodine Radioisotopes; D011480:Protease Inhibitors; D006441:Hemoglobin A; D010447:Peptide Hydrolases