Immunocytochemical localization of ingested kidney bean (Phaseolus vulgaris) lectins in rat gut.
The binding of ingested kidney bean (Phaseolus vulgaris) lectins to the luminal surface of the rat gut was investigated by an indirect immunofluorescence method in which the primary antiserum was monospecific for kidney bean globulin lectins. The major lectin-positive sites were found to be the non-crypt regions of villi in the proximal region of the small intestine; exactly the same regions displayed extensive disruption of microvilli. Lectins were not localized in the brush border regions of ileal villi and no microvillus abnormalities were detected in this region of the gut. The luminal surface of the caecum was strongly lectin-positive and here also microvillus disruption was evident. Low levels of lectins were detected on the luminal surface of the colon; no microvillus abnormalities were detected in this region.
D000818:Animals; D004032:Diet; D005455:Fluorescent Antibody Technique; D005753:Gastric Mucosa / Q000032:analysis*; D007413:Intestinal Mucosa / Q000032:analysis*; D008297:Male; D008871:Microvilli / Q000378:metabolism; D010835:Phytohemagglutinins / Q000032:analysis* / Q000378:metabolism; D011485:Protein Binding; D051381:Rats
D010835:Phytohemagglutinins