Rheumatoid factors in mice: plaque assay for homophile and heterophile rheumatoid factors.
It was found that a high proportion of IgM-producing cells secrete homophile or heterophile rheumatoid factors (RF). Injection of lipopolysaccharide (LPS) into CBA mice resulted in a sharp increase in total IgM and in RF-producing cells and in some cases resulted in an increase in RF-producing cells as a proportion of total IgM. RF plaques are indirect IgM in the sense that they require development by IgG2 (anti-IgM) antibody for visualization in a standard haemolytic plaque assay. The RF response to LPS is both dose- and time-dependent.
D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D006462:Hemolytic Plaque Technique; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D012217:Rheumatoid Factor / Q000096:biosynthesis*; D013997:Time Factors
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D012217:Rheumatoid Factor