A novel method to improve immunofluorescence in histological specimens.
A new approach to immunofluorescent labeling of sectioned tissue is described. Small tissue fragments which have been fixed and rendered permeable are labeled with immune reagents by either the direct or indirect method, prior to embedding in glycol methacrylate (2-hydroxyethyl methacrylate) at 4 degrees. In this way, the well-known superiority of plastic embedding over paraffin embedding or cryo-sections can be used to compare cellular structure in tissues with that seen in cultured cells.
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D003094:Collagen / Q000378:metabolism; D003599:Cytoskeleton / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D008214:Lymphocytes; D011102:Polyhydroxyethyl Methacrylate; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
D011947:Receptors, Antigen, B-Cell; D014404:Tubulin; D011102:Polyhydroxyethyl Methacrylate; D003094:Collagen