Identification by photoaffinity labelling of 16S RNA as a component of IF2-binding site of E. coli MRE 600 ribosome.
A photoactivated radioactive derivative of IF2 was obtained by treatment with p-azidobenzaldehyde in the presence of NaBH4, followed by reductive methylation with [14C] formaldehyde. The modified IF2 preserved the functional activity of the native factor in specific binding to 30S ribosomal subparticles to which it specifically cross-linked during mild irradiation. The main affinity-labelled component of the 30S subparticle was found to be 16S RNA.
D000345:Affinity Labels; D001386:Azides; D001547:Benzaldehydes; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D004926:Escherichia coli / Q000378:metabolism*; D015852:Eukaryotic Initiation Factor-2; D008970:Molecular Weight; D010448:Peptide Initiation Factors / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism* / Q000648:ultrastructure
D000345:Affinity Labels; D001386:Azides; D001547:Benzaldehydes; D015852:Eukaryotic Initiation Factor-2; D010448:Peptide Initiation Factors; D011506:Proteins; D012335:RNA, Ribosomal; C026479:4-azidobenzaldehyde