Metabolic activation of 2-aminofluorene by isolated rat liver cells through different pathways leading to hepatocellular DNA-repair and bacterial mutagenesis.
The aromatic amine 2-aminofluorene (2-AF) is metabolised by isolated rat liver cells to reactive species, thereby causing mutagenic effects in Salmonella typhimurium TA 1538 and evoking DNA-excision repair within the liver cells. The pathway leading to the production of metabolites mutagenic in Salmonella is likely to proceed via direct N-hydroxylation of 2-AF to N-hydroxy-2-aminofluorene (N-OH-2-AF). On the other hand, the formation of intermediates giving rise to hepatocellular DNA-repair is shown to depend upon N-acetylation of 2-AF to 2-acetylaminofluorene(2-AAF), whereas a subsequent conjugation reaction, most likely to be sulfate ester formation, is also essentially involved.
D015073:2-Acetylaminofluorene / Q000378:metabolism; D000818:Animals; D001711:Biotransformation; D004260:DNA Repair / Q000187:drug effects*; D004347:Drug Interactions; D005449:Fluorenes / Q000378:metabolism* / Q000633:toxicity; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009153:Mutagens; D051381:Rats; D012486:Salmonella typhimurium / Q000235:genetics
D005449:Fluorenes; D009153:Mutagens; C012177:2-aminofluorene; D015073:2-Acetylaminofluorene