The effect of delta-aminolevulinate on catalase T-messenger RNA levels in delta-aminolevulinate synthase-defective mutants of Saccharomyces cerevisiae.
Total RNA was isolated from mutants of Saccharomyces cerevisiae that lack active delta-aminolevulinate synthase and are therefore defective in heme biosynthesis. The RNAs were translated in the cell-free protein synthesis system from wheat germ, and the catalase T synthesized was isolated by immunoadsorption and polyacrylamide gel electrophoresis in the presence of dodecyl sulfate. Little or no catalase T product was detected with RNA from mutant cells grown in the absence of a heme precursor. As judged from in vitro translational capacity, RNA fractions from mutant cells grown in the presence of delta-aminolevulinate contained at least 10 times more catalase T mRNA than RNA from unsupplemented cells.
D000624:5-Aminolevulinate Synthetase / Q000235:genetics*; D000622:Aminolevulinic Acid / Q000494:pharmacology*; D002374:Catalase / Q000096:biosynthesis*; D006418:Heme / Q000096:biosynthesis; D007982:Levulinic Acids / Q000494:pharmacology*; D009154:Mutation; D010944:Plants / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D014908:Triticum / Q000378:metabolism
D007982:Levulinic Acids; D012333:RNA, Messenger; D006418:Heme; D000622:Aminolevulinic Acid; D002374:Catalase; D000624:5-Aminolevulinate Synthetase