A simple method for th production of anti-C3 antiserum in rabbits.
A method is described for the production of an anti-C3+d specific antiserum in rabbits. The method does not require purification of C3 but relies on the binding of human C3 to the rabbit erythrocytes following activation of the alternative pathway. The antiserum produced is suitable for use as either a serological reagent or for immunoprecipitation.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D003176:Complement C3 / Q000276:immunology* / Q000378:metabolism; D003170:Complement Pathway, Alternative; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D007158:Immunologic Techniques; D011817:Rabbits
D000906:Antibodies; D003176:Complement C3