Isolation of plasmids carrying either the uvrC or uvrC uvrA and ssb genes of Escherichia coli K-12.
A 3.4 kb PstI fragment containing the uvrC gene of Escherichia coli K-12 has been cloned into pBR322. Plasmids carrying this PstI fragment, in either orientation (pGY3233, pGY4211) relative to the cloning vehicle, complement uvrC mutants. A second plasmid (pGY3243) with a 21 kb HindIII fragment is shown to complement mutations in uvrA and ssb (single-strand binding protein). A composite plasmid (pGY4610) containing pBR322 and PstI fragments derived from pGY3233 (3.4 kb) and pGY3243 (11.05 kb) complements the uvrC, uvrA and ssb mutations.
D003001:Cloning, Molecular; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects; D005796:Genes; D010641:Phenotype; D010957:Plasmids; D011740:Pyrimidine Dimers; D011836:Radiation Tolerance; D014466:Ultraviolet Rays
D011740:Pyrimidine Dimers