Separation of enkephalin degradation products by ligand-exchange chromatography.
Enkephalin degradation products can be accurately analyzed by cascade chromatography through XAD polystyrene and copper--Chelex columns. One of the main degradation products generated during the incubation of enkephalin with rat striatal membranes, the N-terminal amino acid Tyr, is absorbed quantitatively on the copper--Chelex columns whereas the minor, but probably specific, product Tyr--Gly--Gly is not.
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D002614:Chelating Agents; D002852:Chromatography, Ion Exchange / Q000379:methods; D003300:Copper; D003342:Corpus Striatum / Q000201:enzymology; D010450:Endopeptidases / Q000378:metabolism; D004723:Endorphins / Q000378:metabolism*; D004745:Enkephalins / Q000378:metabolism*; D008024:Ligands; D015260:Neprilysin; D010446:Peptide Fragments / Q000032:analysis; D051381:Rats; D012117:Resins, Synthetic
D002614:Chelating Agents; D004723:Endorphins; D004745:Enkephalins; D008024:Ligands; D010446:Peptide Fragments; D012117:Resins, Synthetic; C024997:Chelex 100; D003300:Copper; D010450:Endopeptidases; D015260:Neprilysin