title: Use of the enzyme-linked immunosorbent assay technique (elisa) for the diagnosis of typhoid fever. I. Standardization of technique.
abstract: The sera of 25 patients with bacteriological diagnosis of typhoid fever were analyzed to identify and measure the antibodies to S. typhi somatic antigens using the enzyme-linked immunosorbent assay technique (ELISA). The results were positive in all the serologic tests, with titers varying from 1:600 to 1:2500. Development and standardization of the technique, results of the intra-assay and inter-assay tests and their correlation to Widal's reaction are described in detail.
mesh_terms: D000293:Adolescent; D000907:Antibodies, Bacterial / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards*; D014435:Typhoid Fever / Q000175:diagnosis* / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial