Proteus mirabilis phages 5006M, 5006M HFT k and 5006M HFT ak: physical comparison of genome characteristics.
The genomes of proteus mirabilis phages 5006M, kanamycin resistance transducing variant 5006M HFT k and kanamycin-ampicillin resistance transducing variant 5006M HFT ak have been compared. Homo- and heteroduplex and partial denaturation mapping analyses were performed. The results confirm a sequential headful packaging mechanism, facilitate mapping of the ampicillin resistance marker, demonstrate a hairpin loop structure in both variants, reveal a common insertion site for 8 X 10(6) mol. wt. non-phage DNA in both variants and implicate a role for the non-inducible cryptic host strain prophage 5006M in the generatin cycle of the variant phages.
D000667:Ampicillin / Q000494:pharmacology; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D007612:Kanamycin / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009692:Nucleic Acid Heteroduplexes; D010403:Penicillin Resistance; D011513:Proteus mirabilis
D004279:DNA, Viral; D009692:Nucleic Acid Heteroduplexes; D007612:Kanamycin; D000667:Ampicillin