title: Alcohol and very low density lipoprotein synthesis and secretion by isolated hepatocytes.
abstract: Labeled leucine can be used to measure accurately the rate of both total and secretory protein synthesis by isolated hepatocytes if at least 1 mM leucine is added to the incubation medium, even in the presence of 50 mM ethanol. Using this technique it was found that ethanol caused a significant inhibition of very low density lipoprotein (VLDL) as well as total protein synthetic rates in hepatocytes from both fed and fasted rats. In contrast, a single acute oral dose of ethanol to fasted rats caused within 4 hr a threefold stimulation in the rate of VLDL synthesis without affecting the total protein synthetic rate in the hepatocyte system.
mesh_terms: D000818:Animals; D000431:Ethanol / Q000494:pharmacology*; D005215:Fasting; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism; D008079:Lipoproteins, VLDL / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats
chemical_list: D008079:Lipoproteins, VLDL; D000431:Ethanol; D007930:Leucine