[Detection of "Clostridium difficile" on minimal and selective medium and by immunofluorescence antibody staining (author's transl)].
A minimal and selective medium containing 5 aminoacids, 3 vitamins, a low sugar concentration and 2 antibiotics (cefoxitin at 16 microgram/ml and streptomycin at 500 microgram/ml) is described for isolation of Clostridium difficile from the gut. Comparable results were obtained with this medium and using spore isolation by the sodium thioglycolate-lysozyme technique. An antiserum specific to C. difficile was prepared and used for detection by immunofluorescent antibody staining. This is a very sensitive technique which leads to a quick and early identification of this bacterial species.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D003013:Clostridium / Q000276:immunology / Q000302:isolation & purification*; D006224:Cricetinae; D003470:Culture Media; D004761:Enterocolitis, Pseudomembranous / Q000188:drug therapy / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D006801:Humans
D000900:Anti-Bacterial Agents; D003470:Culture Media
Détection de Clostridium difficule par isolement sur milieu minimal sélectif et par immunofluorescence.