Chromosome replication following a temporary inhibition of DNA-synthesis by nalidixic acid in a temperature-sensitive dnaA mutant of Escherichia coli.
Chromosome replication cycle in a DNA initiation mutant of Escherichia coli (CRT-83, dnaAts) was blocked by nalidixic acid, an inhibitor of the A subunit of DNA gyrase. Following a period of inhibition of DNA synthesis, the drug was removed and "run-out" DNA synthesis was examined. It was found that the "capacity" for DNA synthesis was not affected by such a treatment.
D004261:DNA Replication / Q000187:drug effects*; D004278:DNA, Superhelical / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology*; D013696:Temperature
D004278:DNA, Superhelical; D009268:Nalidixic Acid