Cytophotometric re-investigation of DNA content in Purkinje cells of the rat cerebellum.
Stage scanning cytophotometry of Feulgen-stained cell nuclei of the cerebellum of young adult rats revealed that the absorbance values of the majority of the Purkinje cells show a Gaussian distribution with a low coefficient of variance. The peak absorbance of this population is the same as that of the granule cells. About 1% of the Purkinje cells measured, were found to have a stain content which indicates a 4C amount of DNA. For both the granular and the Purkinje cell population, a very small number of nuclei possesses absorbance values intermediate between 2C and 4C. The present data suggest prevalent diploidy of the Purkinje cells, and are at variance with those postulating a 'tetraploid and/or hyperdiploid' status of this population.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D003584:Cytological Techniques; D004247:DNA / Q000032:analysis*; D004171:Diploidy; D005260:Female; D011689:Purkinje Cells / Q000032:analysis*; D051381:Rats; D013050:Spectrometry, Fluorescence; D013223:Statistics as Topic
D004247:DNA