title: Enzymatic reduction of protein-bound methionine sulfoxide.
abstract: An enzyme that catalyzes the reduction of methionine sulfoxide residues in ribosomal protein L12 has been partially purified from Escherichia coli extracts. Methionine sulfoxide present in oxidize [Met]enkephalin is also reduced by the purified enzyme. The enzyme is different from a previously reported E. coli enzyme that catalyzes the reduction of methionine sulfoxide to methionine [Ejiri, S. I., Weissbach, H. & Brot, N. (1980) Anal. Biochem. 102, 393--398]. Extracts of rat tissues, Euglena gracilis, Tetrahymena pyriformis, HeLa cells, and spinach also can catalyze the reduction of methionine sulfoxide residues in protein.
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D008715:Methionine / Q000031:analogs & derivatives* / Q000378:metabolism; D051150:Methionine Sulfoxide Reductases; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D010944:Plants; D012269:Ribosomal Proteins / Q000378:metabolism*; D013045:Species Specificity; D013454:Sulfoxides / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D012269:Ribosomal Proteins; D013454:Sulfoxides; D008715:Methionine; D010088:Oxidoreductases; D051150:Methionine Sulfoxide Reductases; C027219:methionine sulfoxide reductase; C013111:methionine sulfoxide