title: The removal of egg shell membranes by enzyme treatment to facilitate the study of shell microstructure.
abstract: A procedure is described for the enzymatic hydrolysis of the outer shell membrane of hen eggs using a nonspecific protease. This method is less rigorous than the conventional method of boiling in NaOH and it eliminates the possibility of damage to shell fragments during removal of the adhering membrane.
mesh_terms: D000818:Animals; D004528:Egg Shell / Q000648:ultrastructure; D008566:Membranes / Q000187:drug effects*; D010447:Peptide Hydrolases / Q000494:pharmacology*
chemical_list: D010447:Peptide Hydrolases