Preparation and some chemical characteristics of milk-clotting protease from Bacillus mesentericus 76.
Pure milk-clotting protease (MCP-76) is isolated by isotachophoresis at pH 5.0. The native molecule has only one protein chain. It is a metaloenzyme containing zinc. The pure MCP-76 has a molecular weight of 33 000 (+/- 1500) and by diphenyl-indenonyl-isothiocyanate method showed arginine as N-terminal amino acid.
D000818:Animals; D016282:Aspartic Acid Endopeptidases; D001230:Aspergillus / Q000302:isolation & purification; D001407:Bacillus / Q000201:enzymology*; D002417:Cattle; D010450:Endopeptidases / Q000302:isolation & purification*; D008892:Milk; D008970:Molecular Weight
D010450:Endopeptidases; D016282:Aspartic Acid Endopeptidases; C022517:rennin-like enzyme (Aspergillus ochraceus)