Metabolism of (5E)-6a-carbaprostaglandin I2 by rhesus monkey lung 15-OH prostaglandin dehydrogenase.
(5E)-6a-Carbaprostaglandin I2 (carbacyclin) was oxidized to (5E)-15-dehydro-6a-carbaprostaglandin I2 (15-dehydrocarbacyclin) by partially purified rhesus monkey lung prostaglandin dehydrogenase (PGDH). The (5E-15-dehydro-6a-carbaprostaglandin I2 was isolated by preparative thin-layer chromatography and identified by gas chromatography-mass spectrometry. A Lineweaver-Burke plot gave an apparent Km value of 2.9 microM and a Vmax of 35.7 nmoles carbacyclin oxidized/mg protein/min. These values are similar to previously reported Km and Vmax values for PGI2 and PGE1.
D000818:Animals; D011464:Epoprostenol / Q000378:metabolism*; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism*; D007700:Kinetics; D008168:Lung / Q000201:enzymology*; D008253:Macaca mulatta; D011453:Prostaglandins / Q000378:metabolism*; D011465:Prostaglandins, Synthetic / Q000378:metabolism
D011453:Prostaglandins; D011465:Prostaglandins, Synthetic; D011464:Epoprostenol; D006911:Hydroxyprostaglandin Dehydrogenases