A simple test for the terminal deoxynucleotidyl transferase using the peroxidase-antiperoxidase technique.
A technique has been worked out for an easy detection of terminal transferase in individual cells by means of the unlabeled antibody enzyme (PAP) method. Terminal transferase is an important enzyme marker for the classification of acute lymphoblastic leukemias that show a variable extent of differentiation into the T-cell series. The incubation procedure giving the best results is described.
D004253:DNA Nucleotidylexotransferase / Q000032:analysis*; D004254:DNA Nucleotidyltransferases / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007945:Leukemia, Lymphoid / Q000201:enzymology
D004254:DNA Nucleotidyltransferases; D004253:DNA Nucleotidylexotransferase