Simultaneous localization of intracellular myosin and surface concanavalin A receptor clusters using immuno-electron microscopy.
Serial sectioning and immuno-ferritin plus peroxidase labeling of ultrathin frozen sections have been used for the simultaneous localization at the electron microscopic level of surface concanavalin A (Con A) receptors and the intracellular contractile protein, myosin, in mouse splenic lymphocytes. An accumulation of intracellular myosin directly underneath the Con A-induced receptor clusters has been observed. This myosin accumulation seems to be restricted to within approximately 1,000-1,500 A from the surface of the cell. This result provides additional support for the possible occurrence of transmembrane interactions between surface receptors and intracellular contractile elements.
D000818:Animals; D000937:Antigen-Antibody Reactions; D002462:Cell Membrane / Q000032:analysis; D005293:Ferritins; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000032:analysis* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D009218:Myosins / Q000032:analysis*; D011952:Receptors, Concanavalin A / Q000032:analysis*
D011952:Receptors, Concanavalin A; D005293:Ferritins; D009218:Myosins