Identification of the major metabolite of prostacyclin and 6-ketoprostaglandin F1 alpha in man.
Human volunteers were infused with 3H- and 2H-labeled prostacyclin or 3H-labeled 6-ketoprostaglandin F1 alpha and, in separate experiments, with the unlabeled prostanoids. The urine was purified by different chromatographic steps and finally separated into several fractions by high-performance liquid chromatography. The major fractions contained 20.5 and 23.0% of the eluted readioactivity for the metabolites of prostacyclin and 6-ketoprostaglandin F1 alpha, respectively. The structure of both metabolites was identified by gas-liquid chromatography-mass spectrometry as dinor-6-ketoprostaglandin F1 alpha. It is concluded that the major metabolite of prostacyclin and 6-ketoprostaglandin  F1 alpha in man is dinor-6-ketoprostaglandin F1 alpha.
D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol / Q000378:metabolism* / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D011453:Prostaglandins / Q000378:metabolism*; D011460:Prostaglandins F / Q000378:metabolism* / Q000652:urine; D014316:Tritium
D011453:Prostaglandins; D011460:Prostaglandins F; D014316:Tritium; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol