Characterization and response to mitogens of mammary lymphocytes from the bovine dry-period secretion.
From bovine mammary secretion during the dry period, the total number of cells was between 1.2 and 5.9 X 10(6)/ml. A mean of 35% of these cells were classified as lymphocytes and approximately 85% of them could be isolated by the Ficoll-isopac method. Centrifugation separated 6% of the cells into the fat; 5% of them were lymphocytes. About 47% of the lymphocytes bound Helix pomatia agglutinin, a T-cells marker, while the proportion of Ig-bearing cells was approximately 28%. The mammary lymphocytes were stimulated by the lectins phytohaemagglutinin, pokeweed mitogen, concanavalin A and by lipopolysaccharide from Salmonella typhimurium. The stimulation indices of mammary lymphocytes were generally lower than those for peripheral blood lymphocytes from the same animals. The background values, i.e. counts/min of lymphocytes incubated without mitogen, were often higher for lymphocytes isolated from mammary secretion than from blood.
D000818:Animals; D002417:Cattle / Q000276:immunology*; D003208:Concanavalin A / Q000276:immunology; D005260:Female; D007774:Lactation; D008070:Lipopolysaccharides / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D008321:Mammary Glands, Animal / Q000276:immunology* / Q000378:metabolism; D010835:Phytohemagglutinins / Q000276:immunology; D011043:Pokeweed Mitogens / Q000276:immunology; D011247:Pregnancy; D012486:Salmonella typhimurium
D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D003208:Concanavalin A