title: Plasmid R1 DNA replication dependent on protein synthesis in cell-free extracts of E. coli.
abstract: Bacterial plasmids serve as model systems for studying the regulation of DNA replication. Elucidation of the molecular mechanisms involved in plasmid DNA synthesis requires the development of efficient cell-free plasmid replication systems. Such in vitro systems have previously only been described for Col E1-type plasmids and for the R6K plasmid. Here we report that extracts of Escherichia coli can carry out the complete replication of miniplasmids derived from the antibiotic-resistance plasmid R1. This R1 replication system differs from the previously described ColE1 and R6K systems in its strict dependence on DNA-directed protein synthesis. We believe this to be the first report of the functional coupling of the three fundamental reactions of genetic information transfer (transcription, translation and replication) in a cell-free system.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis*; D002474:Cell-Free System; D004261:DNA Replication / Q000187:drug effects; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004270:DNA, Circular / Q000096:biosynthesis; D004926:Escherichia coli; D007700:Kinetics; D010957:Plasmids; D014176:Protein Biosynthesis / Q000187:drug effects; D012293:Rifampin / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004270:DNA, Circular; D012293:Rifampin