Effects of doxycycline on the phagocytosis of 32 P-labelled Escherichia coli by human polymorphonuclear cells.
A study on the effect of various concentrations (2-100 microgram/ml) of doxycycline on the phagocytic activity of human polymorphonuclear cells was undertaken using a radio-labelled strain of Escherichia coli as test particle. In the presence of 10% normal human serum pretreatment with 50-100 microgram/ml resulted in a 50% increase in cell-associated radioactivity. When omitting serum during the ingestion phase, or conducting the ingestion phase at 4 degree C, the ingestive capability was reduced. 2 microgram/ml present during the ingestion together with 10% normal human serum reduced the ingestive capability significantly, whereas the presence of 10 microgram/ml exerted no significant effect. By the use of 3H-labelled doxycycline the drug was shown to be firmly fixed to the leukocyte monolayer.
D004318:Doxycycline / Q000494:pharmacology*; D004926:Escherichia coli / Q000276:immunology*; D006801:Humans; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects*; D010761:Phosphorus Radioisotopes
D010761:Phosphorus Radioisotopes; D004318:Doxycycline