Cell-cycle analysis of insulin binding and internalization on mouse melanoma cells.
Binding of 125I-labeled insulin to the surface receptors of Cloudman S-91 mouse melanoma cells (CCL 53.1) was studied at various phases (M, G1, S, and G2) in the cell cycle. Insulin-binding activity was persistently present during the cell cycle but the highest activity was noted at the S-phase. The insulin once bound to the cell surface receptors at any phase of the cell cycle was internalized and degraded, presumably through a lysosomal pathway. Insulin-indexing activity of melanoma cells was not affected by melanocyte-stimulating hormone.
D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D007328:Insulin / Q000378:metabolism*; D007399:Interphase; D009074:Melanocyte-Stimulating Hormones / Q000494:pharmacology; D008545:Melanoma; D051379:Mice; D008938:Mitosis; D011972:Receptor, Insulin / Q000378:metabolism*
D007328:Insulin; D009074:Melanocyte-Stimulating Hormones; D011972:Receptor, Insulin