pKJ1, a naturally occurring conjugative plasmid coding for toluene degradation and resistance to streptomycin and sulfonamides.
Pseudomonas sp. TA8 isolated by m-toluate enrichment from an aqueous sample metabolized toluene and m- and p-xylenes via the meta cleavage pathway, and manifested specific resistance to streptomycin and sulfonamides. A variety of experiments revealed that the pKJ1 plasmid of about 150 megadaltons carried by TA8 specified both the toluene and xylene degradative function (the Tol function) and streptomycin/sulfonamide resistance. The deletion of a segment of pKJ1 (about 22 megadaltons) resulted in the loss of the Tol function. pKJ1 was not assigned to Pseudomonas incompatibility group P-1, P-2, P-3, or P-9.
D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005796:Genes; D010957:Plasmids; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics*; D011815:R Factors; D013307:Streptomycin / Q000494:pharmacology*; D013449:Sulfonamides / Q000494:pharmacology*; D014050:Toluene / Q000378:metabolism*
D004269:DNA, Bacterial; D013449:Sulfonamides; D014050:Toluene; D013307:Streptomycin