[Effect of hydrocortisone and insulin on (14C)-glycine and (3H)-methionine incorporation into spleen proteins in alloxan-diabetic rats].
The intensity of labelled amino acid incorporation into spleen total proteins of albino rat with experimental diabetes was studied as influenced by hydrocortisone, insulin and both hormones simultaneously. Hydrocortisone inhibits and insulin stimulates the [14C] glycine and [3H] methionine incorporation into total proteins of alloxan-diabetic rat spleen. Under simultaneous administration of both hormones the inhibitory hydrocortisone effect is allayed by insulin. It is suggested that an increased level of glucocorticoid hormones in blood is one of the reasons of protein metabolism disturbance under diabetes mellitus.
D000818:Animals; D002250:Carbon Radioisotopes; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D008715:Methionine / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats; D013154:Spleen / Q000187:drug effects / Q000378:metabolism*; D014316:Tritium
D002250:Carbon Radioisotopes; D007328:Insulin; D014316:Tritium; D008715:Methionine; D005998:Glycine; D006854:Hydrocortisone
Vliianie gidrokortizona i insulina na vkliuchenie metki iz [14C] glitsina i [3H] metionina v belki selezenki krys dri alloksanovom diabete.