title: Two types of luliberin-immunoreactive perikarya in the preoptic area of the rat.
abstract: At the light microscopic level, following immunostaining with a single antiserum against luliberin (LRF), two types of hormone-producing perikarya in the preoptic area are demonstrated. The two cell types differ in their morphological features: a bipolar, smooth-contoured cell type can be differentiated from an irregularly contoured unipolar type. Intermediate forms between both cell types occurring in the same area are not observed. Electron microscopically, both cell types contain labeled granules of similar size and immunoreactivity. It is discussed whether the uneven surface of the one cell type is due to areas of synaptic contacts, and whether both cell types are integrated in different neuronal and functional circuits. Moreover, at the ultrastructural level, from the irregularly contoured LRF-producing perikarya a further positively stained cell type, probably a glial cell, can be differentiated. The specific labeling of the latter is caused by its content of immunoreactive lysosomal bodies. Differentiation between the labeled glial cells and the irregularly contoured LRF-producing perikarya is not possible at the light microscopic level.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000096:biosynthesis*; D007031:Hypothalamus / Q000166:cytology*; D007124:Immunoenzyme Techniques; D008297:Male; D009457:Neuroglia / Q000648:ultrastructure; D009940:Organoids / Q000648:ultrastructure; D011301:Preoptic Area / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D013569:Synapses / Q000648:ultrastructure
chemical_list: D007987:Gonadotropin-Releasing Hormone