[Conditions of the synthesis of lysine decarboxylase by Escherichia coli MRE 600].
The effect of aeration, pH of the cultivation medium, temperature and time of cultivation of Escherichia coli MRE600 on the synthesis of cellular lysine decarboxylase (LDC) was studied. It was demonstrated that LDC reached maximum activity in the 5-hour bacterial culture cultivated in the nutrient medium with pH 6.0 at 29--30 degrees C. Aeration increased the yield by about 3 times and decreased LDC activity by 5.5 times.
D000332:Aerobiosis; D002262:Carboxy-Lyases / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development; D007700:Kinetics; D013696:Temperature
D002262:Carboxy-Lyases; C021520:lysine decarboxylase
Usloviia obrazovaniia lizindekarboksilazy Escherichia coli MRE 600.