title: Properties of ribosomes and ribosomal RNAs synthesized by Escherichia coli grown in the presence of ethionine. Normal maturation of ribosomal RNA in the absence of methylation.
abstract: Analysis of 16-S rRNA synthesized in Escherichia coli D10 (met-) incubated in a medium containing ethionine in place of methionine shows that it lacks most and probably all of the methyl groups present in normal 16-SrRNA but possesses the same 3'-OH, and 5'-phosphate terminal sequences as the latter. 23-S rRNA formed in ethionine-treated cells also contains normal terminal sequences. 5-S rRNAs of normal and ethionine-treated E. coli D10 are identical. These results lead to the conclusion that methylation of ribosomal precursor RNAs is not necessary for their maturation to products with normal chain lengths and does not influence the conformation of 16-S rRNA.
mesh_terms: D004926:Escherichia coli / Q000378:metabolism*; D005001:Ethionine / Q000378:metabolism*; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D008745:Methylation; D008970:Molecular Weight; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D012265:Ribonucleotides / Q000032:analysis; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D012335:RNA, Ribosomal; D012265:Ribonucleotides; D008715:Methionine; D005001:Ethionine