[Influence of alcohol lipotropic agents on biosynthesis and repression of secreted alkaline phosphatase in Escherichia coli].
Preincubation of cells in the presence of 4% ethanol accompanied by an increase of non-saturated cis-vaccenic acid content was shown to promote synthesis of alkaline phosphatase. Preincubation of cells in 0.1% hexanol reducing the level of this acid, on the contrary, leads to partial repression of the enzyme synthesis; the lag-phase of repression in the cells with a raised content of non-saturated cis-vaccenic acid and, consequently, with a greater fluidity of lipids was also shown to be reduced. Conversely, the reduction of lipid membrane fluidity on ethanol addition simultaneously with the repressing metabolite ortho-phosphate extends the lag-phase of repression and removes it partially during cell cultivation in the presence of ortho-phosphate. The impact of lipid composition variations on the synthesis and repression of alkaline phosphatase is discussed.
D000438:Alcohols / Q000494:pharmacology*; D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004794:Enzyme Repression / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D008560:Membrane Fluidity / Q000187:drug effects; D013329:Structure-Activity Relationship
D000438:Alcohols; D000469:Alkaline Phosphatase
Vliianie lipotropnykh agentov spirtov na biosintez i repressiiu sekretiruemoĭ shchelochnoĭ fosfatazy u Escherichia coli.