Enhancement of hemopoietic recovery after bone marrow transplantation with the addition of nucleated blood cells in mice.
Recovery of bone marrow cellularity, CFU-C, and CFU-S were studied sequentially over 90 days time after syngeneic bone marrow transplantation in mice. A minimal cell dose of 2 X 10(5) bone marrow cells was given. At day 28 after transplantation, CFU-C reached more than 50% of the normal range whereas the CFU-S concentration was less than 15%. Normalization of CFU-S occurred at day 90. The effect of the addition of peripheral blood nucleated cells on bone marrow hemopoietic recovery was studied at day 28. The augmentation of CFU-C and CFU-S recoverey were dose dependent. Optimal enhancement was seen with bone marrow to blood ratios of 1:1 and 1:2.5. This enhancement effect was lost when nucleated blood cells in a ratio of 1:10 were administered.
D000818:Animals; D001773:Blood Cells / Q000637:transplantation*; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002467:Cell Nucleus; D003114:Colony-Forming Units Assay; D006410:Hematopoiesis; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology; D014185:Transplantation, Isogeneic