Overproduction of outer membrane protein suppresses envA-induced hyperpermeability.
A quantitative study on outer membrane components was performed in a number of envelope mutants of Escherichia coli K-12 exhibition different permeability properties for antimicrobial agents. The envA1 allele causing an increased influx for both hydrophobic and hydrophilic drugs was found to be associated with a deficiency in the amount of lipopolysaccharides. The sefA1 envA1 double mutant was found to have a higher outer membrane buoyant density, apparently due to an increase in protein content. This double mutant was still low in lipopolysaccharide content.
D000483:Alleles; D000900:Anti-Bacterial Agents / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002463:Cell Membrane Permeability; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis*; D009154:Mutation; D010743:Phospholipids / Q000378:metabolism; D013489:Suppression, Genetic
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D010743:Phospholipids