Flow cytometric analysis of normal and neoplastic human hematopoietic surface antigens.
Normal and neoplastic human hematopoietic cells were examined for surface markers by a variety of techniques including cytotoxicity assays using anti-HTLA and anti-Ia antiserum with viability measured by trypan blue exclusion and automated flow cytometry; E- and EAC-rosette binding assays and surface immunoglobulin measured by a fluorescence-activated cell sorter. In most cases there was good agreement among these assays. However, one case of CLL of T origin (92% E-rosette-positive) also showed significant amounts of Ia antigen by cytotoxicity tests; additionally, a case of CML in blast crisis demonstrated no E or EAC markers or surface immunoglobulin, but the majority of cells were lysed by both anti-HTLA and anti-Ia antiserum. Thus, Ia is not an exclusive B cell marker.
D000818:Animals; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D001665:Binding Sites; D002471:Cell Transformation, Neoplastic; D003602:Cytotoxicity, Immunologic; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007158:Immunologic Techniques; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell; D014343:Trypan Blue / Q000494:pharmacology
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D007106:Immune Sera; D011947:Receptors, Antigen, B-Cell; D014343:Trypan Blue