The detection of mutagenic air pollutants from filter samples by the Salmonella/Mammalian S-9 mutagenicity test (Ames test) with S. typhimurium TA 98 (Part 1).
Particulate airborne pollutants, collected in 1977 at an urban point in Wanne-Eickel, G.F.R., were investigated for mutagenic activity in the Ames Salmonella/mammalian S-9 mutagenicity tet with S. typhimurium TA98. Two kinds of filters were used: Mikrosorbanfilters (polystyrene) and Membranfilters (cellulose nitrate). Sample preparations obtained following gel chromatography of solutions of the Mikrosorbanfilters showed only weak mutagenic activity. Soxhlet extracts of the Membranfilters gave positive dose-response relations (figs. 3-6), while splitting the initial extract into six fractions resulted in the bacterial reversion rates due to certain sub-fractions increasing over the reversion rates due to the unfractionated preparation (fig. 7). The highest activities were observed in the fractions of the polar compounds and of the polycyclic aromatic hydrocarbons. Some (30-50%) of the mutagenic activity was not enzymatically mediated. GC-MS analysis of the samples demonstrated the presence of an average content of around 11 microgram benzo(a)-pyrene/1000 m3 of air.
D000388:Air / Q000032:analysis; D000393:Air Pollutants / Q000032:analysis*; D000818:Animals; D001580:Benzopyrenes / Q000032:analysis; D001711:Biotransformation; D005374:Filtration / Q000295:instrumentation; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000201:enzymology; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000032:analysis*; D009154:Mutation; D012486:Salmonella typhimurium / Q000235:genetics
D000393:Air Pollutants; D001580:Benzopyrenes; D009153:Mutagens