Decrease in the S1 protein of 30-S ribosomal subunits in polyamine-requiring mutants of Escherichia coli grown in the absence of polyamines.
The reason for the decrease of polypeptide-synthetic activity of 30-S ribosomal subunits obtained from two polyamine-requiring mutants of Escherichia coli, grown in the absence of polyamines, has been studied by analyzing the total and split proteins of 30-S subunits by disc gel and slab gel electrophoresis. It was concluded that the decrease of S1 protein in 30-S subunits was responsible for the decrease of polypeptide synthesis in polyamine-requiring mutants of E. coli grown in the absence of polyamines.
D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D010452:Peptide Biosynthesis; D011073:Polyamines / Q000494:pharmacology*; D012269:Ribosomal Proteins / Q000502:physiology*
D011073:Polyamines; D012269:Ribosomal Proteins; C015494:ribosomal protein S1