Preparation of a highly purified vaccine against tick-borne encephalitis by continuous flow zonal ultracentrifugation.
By the use of continuous-flow zonal ultracentrifugation, a highly purified, inactivated tick-borne encephalitis (TBE) virus vaccine was prepared tht contained almost exclusively TBE virus particles. The purity of this preparation was 90- to 100-fold higher than that of a previously used partially purified vaccine. A mean protein content of 3-5 microgram/ml in the final vaccine represents 20 mouse protective doses50 in a mouse challenge potency test. This high degree of purity resulted in a strong reduction of side reactions observed with the less purified vaccine.
D000818:Animals; D000917:Antibody Formation; D000956:Antigens, Viral / Q000032:analysis; D002499:Centrifugation, Density Gradient; D004675:Encephalitis, Tick-Borne / Q000276:immunology*; D007122:Immunoelectrophoresis; D007124:Immunoenzyme Techniques; D051379:Mice; D011506:Proteins / Q000032:analysis; D014461:Ultracentrifugation / Q000379:methods*; D014765:Viral Vaccines / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*
D000956:Antigens, Viral; D011506:Proteins; D014765:Viral Vaccines