Influence of growth environment on the phosphoenolpyruvate: glucose phosphotransferase activities of Escherichia coli and Klebsiella aerogenes: a comparative study.
A consistent difference was found between glucose-limited cultures of Escherichia coli and Klebsiella aerogenes strains in the manner which their apparent cellular content of glucose:phosphoenolpyruvate phosphotransferase (glucose-PTS) varied with growth rate. With the former strains, activity increased as a function of growth rate; in the latter it decreased. However, under glucose-sufficient conditions (potassium- or ammonia-limitation) both species behaved similarly; the glucose-PTS activity was lower and bore no obvious relationship to the rate of glucose consumption expressed by the growing culture. These results are discussed in relation to the role of glucose as a regulator of glucose-PTS synthesis, and to the likely contribution which the glucose-PTS makes to the overall rate of glucose uptake, particularly by cells gorwing in glucose-sufficient environments.
D000641:Ammonia / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000378:metabolism; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism
D000641:Ammonia; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D005947:Glucose; D011188:Potassium