[Steroid transformation with immobilized microorganisms. I. Transformation of cholesterol to cholestenone in organic solvents].
The transformation of cholesterol to cholest-4-ene-3-one has been investigated in the presence of toluene and carbontetrachloride using cells of Nocardia erythropolis (IMET 7185) immobilized by different methods. The adsorption on DEAE-cellulose has been observed to be the most effective method. The stability of immobilized cells relating to cholesteroloxydase activity, transformation capacity, and the influence of water have been investigated.
D000178:Acrylamides; D000327:Adsorption; D000362:Agar; D001431:Bacteriological Techniques; D002251:Carbon Tetrachloride / Q000494:pharmacology; D002782:Cholestenes / Q000096:biosynthesis*; D002783:Cholestenones / Q000096:biosynthesis*; D002784:Cholesterol / Q000378:metabolism*; D003636:DEAE-Cellulose; D005782:Gels; D009615:Nocardia / Q000378:metabolism*; D014050:Toluene / Q000494:pharmacology
D000178:Acrylamides; D002782:Cholestenes; D002783:Cholestenones; D005782:Gels; D014050:Toluene; D000362:Agar; D003636:DEAE-Cellulose; D002784:Cholesterol; D002251:Carbon Tetrachloride
Steroidtransformation mit immobilisierten Mikroorganismen. I. Transformation von Cholesterin zu Cholestenon in organischen Lösungsmitteln.