On the promoter complex formation rate of E. coli RNA polymerases with T7 phage DNA.
Influence of ionic strength on the kinetics of the promoter complex formation between E. coli RNA polymerase and T7 phage DNA was investigated using a membrane filter assay. The enzyme-promoter association rate constant was determined. It varies from 10(9) to 3 x 10(7) M-1 sec-1 when the ionic strength is changed from zero to 0.15 M NaCl. Basing on the theoretical analysis of experimental data obtained the model for the promoter site selection assuming the enzyme sliding along the DNA is discussed.
D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D008956:Models, Chemical; D009876:Operon / Q000187:drug effects; D009994:Osmolar Concentration; D013604:T-Phages / Q000378:metabolism*
D004279:DNA, Viral; D003609:Dactinomycin; D012321:DNA-Directed RNA Polymerases