Divalent cations increase [3H]-prostacyclin binding to membranes of neuronal somatic hybrid cells.
1 [3H]-prostacyclin binding to membranes of a highly differentiated neuronal somatic hybrid is increased equally in the presence of Ca2+, Ba2+, Mg2+ or Sr2+ ions. 2 Analysis of the binding of [3H]-prostacyclin in the presence of low (1 mM) or high (50 mM) Mg2+ ion concentrations has revealed a cation-dependent increase in ligand receptor affinity (Kd values = 57.4 nM and 21.9 nM). 3 The increase in [3H]-prostacyclin binding due to divalent cations is not accompanied by an alteration in the maximum binding capacity of the membranes.
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D011464:Epoprostenol / Q000378:metabolism*; D006822:Hybrid Cells / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology*; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D011453:Prostaglandins / Q000378:metabolism*; D011982:Receptors, Prostaglandin / Q000378:metabolism; D014316:Tritium
D011453:Prostaglandins; D011982:Receptors, Prostaglandin; D014316:Tritium; D011464:Epoprostenol; D008274:Magnesium; D002118:Calcium