Expression of binding sites for peanut agglutinin during murine B lymphocyte differentiation.
Haemopoietic cells from several murine organs were examined for the presence of peanut agglutinin (PNA) receptors. Foetal liver and adult bone marrow contained a number of cells, which were PNA+ but did not stain with conventional T and B markers. Some of the PNA+ cells, however, were pre-B cells, as shown by the presence of cytoplasmic IgM in the absence of cell surface Ig. Cells in the T lineage retained their PNA receptors during maturation, although these became masked by sialic acid on mature peripheral blood T cells. Cells of the B lineage, in contrast, gradually lost their PNA receptors as they matured from pre-B to mature surface immunoglobulin positive B lymphocytes.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D037102:Lectins / Q000276:immunology*; D051379:Mice; D009439:Neuraminidase / Q000494:pharmacology; D011947:Receptors, Antigen, B-Cell; D011975:Receptors, Mitogen / Q000032:analysis*; D013154:Spleen / Q000276:immunology
D037102:Lectins; D011947:Receptors, Antigen, B-Cell; D011975:Receptors, Mitogen; D009439:Neuraminidase