Cells from normal brain and gliomas synthesize pregnancy-specific beta 1-glycoprotein-like material in vitro.
The synthesis of pregnancy-specific beta 1-glycoprotein (SP1) was studied in normal brain-derived and malignant glial-cell cultures. The normal brain-derived and glioma cells were found to contain SP1 when studied by radioimmunoassay and by the triple-bridge immunoperoxidase technique. The active synthesis of SP1 by these brain-derived cells was confirmed by metabolic labelling and subsequent immune precipitation of the culture medium. The SP1-like material produced by the brain-derived cells had the same molecular weight as purified placental SP1.
D001921:Brain / Q000378:metabolism; D001932:Brain Neoplasms / Q000378:metabolism*; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D011257:Pregnancy Proteins / Q000096:biosynthesis*; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000096:biosynthesis*
D011257:Pregnancy Proteins; D011268:Pregnancy-Specific beta 1-Glycoproteins