International collaborative study on the assay of commerically available high and low molecular weight urokinases.
Urokinases of different molecular weights are now commerically available. An international collaborative study (eight laboratories) has been conducted to investigate the effect of type and concentration of plasminogen on the assay of two different urokinase preparations against the International Reference Preparation (IRP). Considerable inter-laboratory variation in relative potency estimation was found, and a small effect of plasminogen concentration, independent of type, was apparent.
D001688:Biological Products / Q000032:analysis*; D010450:Endopeptidases / Q000032:analysis*; D006801:Humans; D007391:International Cooperation; D008970:Molecular Weight; D010958:Plasminogen / Q000494:pharmacology; D012015:Reference Standards; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis*
D001688:Biological Products; D010958:Plasminogen; D010450:Endopeptidases; D014568:Urokinase-Type Plasminogen Activator