title: A comparison of the macrophage electrophoretic mobility test and the leukocyte migration inhibition test in the detection of histocompatibility antigens on tumor cells.
abstract: Lymph node cells from mice sensitized by rejection of skin allografts were incubated in vitro with tumor cells carrying the sensitizing alloantigens. Products of the lymphocyte--antigen interaction released into the culture medium were simultaneously assessed by the macrophage electrophoretic mobility test and the leukocyte migration inhibition assay. Both macrophage slowing factor (MSF) and a factor which inhibited leukocyte migration (LIF) were produced. The release of both factors was detectable after comparable periods of incubation; their production was transient and ceased within 24 hours after it started.
mesh_terms: D000818:Animals; D002464:Cell Migration Inhibition; D004586:Electrophoresis; D006649:Histocompatibility Antigens / Q000032:analysis*; D007961:Leukocyte Migration-Inhibitory Factors / Q000378:metabolism; D007962:Leukocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology*
chemical_list: D006649:Histocompatibility Antigens; D007961:Leukocyte Migration-Inhibitory Factors