[Isolation of yeast protoplasts using various preparations of the hepato-pancreatic juice of Helix pomatia].
Conversion of large amounts of Saccharomyces cerevisiae cells to protoplasts is studied using various preparations extracted from Helix pomatia hepato-pancreatic juice. The most favourable yield in two hours incubations (88 per cent) is obtained with 20 ml cytohelicase, a chitinase and glucanase enriched extract, per 400 g of yeast cells, harvested at the end of the logarithmic growth phase and preincubated in presence of 2-mercaptoethanol.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D006372:Helix, Snails / Q000032:analysis* / Q000201:enzymology; D008099:Liver / Q000032:analysis; D010189:Pancreatic Juice / Q000032:analysis / Q000201:enzymology; D011523:Protoplasts / Q000648:ultrastructure*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000648:ultrastructure*
Obtention de protoplastes de levure à l'aide de différences préparations de suc hépato-pancréatique d'Helix pomatia.