5-(carboxymethylaminomethyl)-2-thiouridine, a new modified nucleoside found at the first letter position of the anticodon.
The structure of a modified uridine derivative which was detected at the first letter position of the anticodon of Bacillus subtilis tRNA1Lys was determined to be 5-(carboxymethylaminomethyl)-2-thiouridine. The determination was mainly based in this ultraviolet absorption spectra and mass spectrometric analysis of the trimethylsilyl derivative.
D000926:Anticodon; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000032:analysis*; D013058:Mass Spectrometry; D012343:RNA, Transfer; D013056:Spectrophotometry, Ultraviolet; D013891:Thiouridine / Q000031:analogs & derivatives* / Q000032:analysis
D000926:Anticodon; D013891:Thiouridine; C030546:5-(carboxymethylaminomethyl)-2-thiouridine; D012343:RNA, Transfer