A method for the preparation of Vicia villosa lectin and a rosette procedure for fractionation of lectin-binding lymphocytes.
We describe a simple procedure for isolation of Vicia villosa lectin with binding specificity for N-acetyl-D-galactosamine greater than D-galactose. Also, a rosette assay using V. villosa lectin coupled-SRBC is described which enables us to both visualize lectin binding cells and to fractionate lymphocytes from vitro primary mixed lymphocyte cultures on the basis of ability to bind V. villosa lectin.
D000116:Acetylgalactosamine / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D002469:Cell Separation; D003602:Cytotoxicity, Immunologic; D005260:Female; D007158:Immunologic Techniques; D037102:Lectins / Q000302:isolation & purification* / Q000378:metabolism; D008214:Lymphocytes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D012397:Rosette Formation; D012756:Sheep
D037102:Lectins; D000116:Acetylgalactosamine