Neighbour and temperature effects on base-analogue-induced mutation in phage T4.
The effects of neighbouring base pairs and of temperature on mutation frequencies were measured at nonsense sites in the T4rII region. 2AP-induced AT leads to GC transition frequencies are insensitive to nearest-neighbour effects, while 5BU-induced ones are promoted by GC neighbours on the 5' side. The effect of temperature on 2AP- and 5BU-induced mutation frequencies shows no simple dependence on nearest neighbours. These results are incompatible with a unitary mechanism as explanation for the effects of nearest neighbours and temperature on base-analogue-induced mutagenesis.
D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D006358:Hot Temperature; D009154:Mutation; D013604:T-Phages / Q000235:genetics*
D004279:DNA, Viral