[A contribution to the technique of immunofluorescence in leptospirosis (author's transl)].
Mono- and polyvalent FITC-conjugates were prepared for detection of leptospires in artifically infected material. The organisms were concentrated by centrifugation and fixed onto glass slides after they had been mixed with serum or egg albumin. By this method, leptospires were detected down to a concentration of 10(2)-10(4) organisms/ml.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007919:Leptospira / Q000276:immunology*; D007922:Leptospirosis / Q000662:veterinary*; D011817:Rabbits / Q000276:immunology; D013045:Species Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
D000942:Antigens, Bacterial; D007106:Immune Sera
Ein Beitrag zur Durchführung der Fluoreszenzserologie bei Leptospirose.