The effect of the oxidizing agents chloramine-T and cigarette smoke on dog serum proteinase inhibitor(s).
Dog serum treated with the oxidant chloramine-T is rapidly and selectively depleted of its ability to inhibit porcine pancreatic elastase or dog neutrophil elastase. Trypsin inhibitory capacity of serum is not affected. Purified dog alpha-1-proteinase inhibitor (alpha-1-PI) is similarly oxidized with an apparent rate constant of 1.1 x 10(3) M-1 sec-1. Reversal of the oxidative inactivation using dithiothreitol was demonstrated. Cigarette smoke also directly affects the inhibitory capacity of both serum and pure alpha-1-PI. These studies form a basis for developing a model of functionally deficient alpha-1-PI by taking advantage of oxidative inactivation of normal proteinase inhibitor levels.
D000818:Animals; D002700:Chloramines / Q000378:metabolism*; D004285:Dogs; D010450:Endopeptidases / Q000097:blood*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D010947:Plants, Toxic; D012906:Smoke; D014026:Nicotiana; D014361:Trypsin Inhibitors
D002700:Chloramines; D012906:Smoke; D014361:Trypsin Inhibitors; D010450:Endopeptidases; D010196:Pancreatic Elastase