Testing of drugs for combined mutagenesis with sodium nitrite in the host-mediated assay.
Mutagenic activity of the drugs analgine and aminophenazone was tested in the intraperitoneal and intrasanguine host-mediated assay after oral application together with equimolar doses of sodium nitrite. Salmonella typhimurium strain G46 was used as genetic indicator system; mice served as host animals for the bacteria. Analgine was found to be weakly mutagenic in the dose 2 mM/kg together with nitrite, aminophenazone was a strong mutagen in combination with nitrite in the doses 2 mM/kg and 0.2 mM/kg using the intrasanguine test with 1 h incubation of bacteria in the liver. In the intraperitoneal variant with 3 h incubation time of bacteria only aminophenazone was slightly mutagenic at the highest dose tested, 2 mM/kg. The relative nitrosation rate for aminophenazone was calculated by means of regression lines for mutagenic activity of dimethylnitrosamine and was found to be in the range of 2% in both systems for a dose of 2 mM/kg precursors.
D000632:Aminopyrine / Q000031:analogs & derivatives / Q000633:toxicity; D000818:Animals; D004177:Dipyrone / Q000633:toxicity; D004347:Drug Interactions; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D009153:Mutagens; D009573:Nitrites / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000235:genetics; D012977:Sodium Nitrite / Q000633:toxicity*; D014116:Toxicology
D009153:Mutagens; D009573:Nitrites; D000632:Aminopyrine; D004177:Dipyrone; D012977:Sodium Nitrite