title: Nucleoside-stimulated insulin production by isolated mouse pancreatic islets.
abstract: Purine ribonucleosides were found to be as potent stimulators of proinsulin biosynthesis as glucose, adenosine being effective at very low concentrations (0.1 mM). Some pyrimidine nucleosides together with 2-deoxyribonucleoside (thymidine) were also potent stimulants. The single components of nucleosides (pentoses and bases) had no, or only slight, stimulatory effects. Nucleosides added to a low-glucose culture medium were found to replace glucose as a long-term stimulant of the insulin biosynthesis of islets in culture. Adenosine-stimulated insulin secretion from perifused islets was biphasic, although kinetic differences were observed as compared with glucose stimulation. The rate of oxidation of [U-14C]-adenosine was dose dependent, whereas no production of 14CO2 was obtained during incubation of islets in [8-14C]-adenosine. Oxidation of 14C-labelled glucose was depressed by addition of adenosine. The results add further support to the view that nucleoside-stimulated insulin secretion and biosynthesis are modulated through metabolic signals.
mesh_terms: D000241:Adenosine / Q000502:physiology; D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D005947:Glucose / Q000502:physiology; D007328:Insulin / Q000096:biosynthesis*; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D051379:Mice; D009705:Nucleosides / Q000502:physiology*; D010084:Oxidation-Reduction; D010477:Perfusion
chemical_list: D007328:Insulin; D009705:Nucleosides; D005947:Glucose; D000241:Adenosine