[Use of the immunoperoxidase method for the rapid detection of togaviruses, orthopoxviruses and their antibodies].
The successful application of the immunoperoxidase method of (IP) with light microscopy for rapid detection of certain togaviruses and orthopoxviruses and antibodies to them is described. A comparative evaluation of the IP and immunofluorescent (IF) method for these purposes was performed. The results indicate practically similar sensitivity and specificity of both methods. The detection of viral antigen directly depended upon the infective dose and time of examinations (within 4--12 hours postinoculation). In evaluating the IP method, however, the account should be taken of the fact that it does not require expensive equipment and therefore may be used in any practical laboratory or even under field conditions. Rapid detection of both viral antigens and antiviral antibody by this method opens approaches for its wide application in the future.
D000914:Antibodies, Viral / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D011212:Poxviridae / Q000276:immunology / Q000302:isolation & purification*; D013997:Time Factors; D014035:Togaviridae / Q000276:immunology / Q000302:isolation & purification*
D000914:Antibodies, Viral
Primenenie immunoperoksidaznogo metoda dlia bystroĭ indikatsii nekotorykh togavirusov, ortopoksvirusov i antitel k nim.