Indirect haemagglutination for the demonstration of antibodies to renal antigens from rats.
An indirect haemagglutination (IHA) test using tanned sheep erythrocytes was developed to detect antibodies in sera from rabbits immunized with renal tubular epithelium (RTE) or with glomerular basement membrane (GBM) from rats. The RTE antigen preparation, called Fx1A, sensitized the erythrocytes directly, whereas the GBM preparation had to be treated with collagenase. THe antisera to Fx1A gave titres up to 64,000, and the antisera to GBM up to several million. The sera were monospecific, since they gave no cross-reactions in IHA, precipitation, or absorption tests. Using the IHA test, the quantitative aspects of experimental glomerulonephritis in rats can be studied. Preliminary results obtained in transfer experiments indicate a dose-response relationship between the titres in IHA and the degree of proteinuria.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000302:isolation & purification; D001485:Basement Membrane / Q000276:immunology; D004848:Epithelium / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005921:Glomerulonephritis / Q000276:immunology; D006386:Hemagglutination Tests; D007668:Kidney / Q000276:immunology*; D007678:Kidney Glomerulus / Q000276:immunology; D007684:Kidney Tubules / Q000276:immunology; D008297:Male; D051381:Rats
D000906:Antibodies; D000941:Antigens