[Studies on E. coli and Kl. pneumoniae on the question of the loss of resistant determinants and the influence of the plasmids RP1 and R100 on the behaviour of these germs in dry and liquid atmopshere with minimal nutritive substances (author's transl)].
It had to be examined if the plasmids have influence on the process of dying or growing of germs in dry or moist atmosphere. Besides it was of interest if the segregation of resistant determinants were to be observed. So the plasmids RP1 or R100 were transferred by conjugation on 5 strains of E. coli and 3 strains of Kl. pneumoniae; for the further examination each strain with RP1 or R100 or without one of these plasmids was available. With these strains examinations on aluminium foil and in aqua bidest at 30 and 40 degrees C were implemented; in another test aqua bidest was enriched with Standard-I-Nutrient-Broth (Merck). By determining the colony forming units in different times we had curves of dying or growing of the germs. After each determination we examined 48 colonies on the loss of resistant determinants. No influence on the process of dying or growing of the strains by plasmids was to be found under the given conditions. In no case loss of resistant determinants was to be checked.
D001431:Bacteriological Techniques; D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000254:growth & development*; D005144:F Factor; D007711:Klebsiella pneumoniae / Q000254:growth & development*; D010957:Plasmids
Untersuchungen an E. coli und Kl. pneumoniae zur Frage des Verlustes von Resistenzdeterminanten und des Einflusses der Plasmide RP1 und R100 auf das Verhalten der Keime in trockenem und nährstoffarmem wässrigen Milieu.