Selective growth of rabbit corneal epithelial cells in culture and basement membrane collagen synthesis.
Selective isolation of rabbit corneal epithelial cells was achieved with a medium containing D-valine. Long-term cultures of the epithelial cells synthesize basement membrane-type pro-collagen doublet chains of 160,000 and 180,000 daltons and a higher-molecular-weight species at 280,000 daltons. Some of these components are disulfide-linked. These peptides gave rise to a single pepsin-resistant collagen chain of approximate molecular size of 115,000 daltons.
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D003315:Cornea / Q000254:growth & development*; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D008970:Molecular Weight; D011817:Rabbits; D014633:Valine
D003094:Collagen; D014633:Valine