Active transport of [32P]thiamine diphosphate in Escherichia coli.
Active uptake of [32P]thiamine diphosphate by E. coli was analyzed using an improved method of gel filtration chromatography. The radioactive coenzyme was accumulated without dephosphorylation. From this result it was concluded that thiamine kinase is not involved in the membrane transport of thiamine in E. coli.
D001693:Biological Transport, Active; D002850:Chromatography, Gel; D004926:Escherichia coli / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D013831:Thiamine / Q000378:metabolism; D013835:Thiamine Pyrophosphate / Q000378:metabolism*
D010761:Phosphorus Radioisotopes; D013835:Thiamine Pyrophosphate; D013831:Thiamine