Description of a coverslip holder for, and observations on, the critical point drying of cell cultures.
The elimination of artefact during the preparation of cell cultures for scanning electron microscopy is difficult. Collapse of cellular projections, cytoplasmic cracks, perforations and fracturing of cell-cell processes and cell-substrate attachments occur during fixation, dehydration and critical point drying. Coating and storage may cause further artefact. A specimen holder which serves to minimize turbulence in the critical point dryer and which allows for the simultaneous processing of up to five coverslips, as well as a reproducible technique for the preparation of cell cultures are described.
D002460:Cell Line; D003584:Cytological Techniques / Q000295:instrumentation*; D003890:Desiccation; D006801:Humans; D008855:Microscopy, Electron, Scanning; D013048:Specimen Handling / Q000295:instrumentation