[Affinity modification of creatine kinase from rabbit skeletal muscles using gamma-(p-azidoanilide)-ATP].
Ultraviolet irradiation of creatine kinase (adenosine 5'-triphosphate: creatine-N-phosphotransferase, EC 2.7.3.2) in the presence of gamma-(p-azidoanilide)-[14C]-ATP results in a complete enzyme inactivation and covalent binding of two moles of analog per mole of enzyme. MgADP strongly protects the enzyme against inactivation. However, in the presence of MgADP the covalent binding of 0.9 moles of analog per mole of enzyme occurs without the enzyme inactivation. The rate of enzyme inactivation and covalent modification decreases in the presence of MgATP and creatine. These results indicate that gamma-(p-azidoanilide)-ATP is an affinity label for the active site of creatine kinase.
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D000345:Affinity Labels / Q000494:pharmacology*; D000818:Animals; D001386:Azides; D002250:Carbon Radioisotopes; D003402:Creatine Kinase / Q000378:metabolism*; D007700:Kinetics; D009132:Muscles / Q000201:enzymology*; D011817:Rabbits; D011865:Radioisotope Dilution Technique
D000345:Affinity Labels; D001386:Azides; D002250:Carbon Radioisotopes; C024294:ATP gamma-p-azidoanilide; D000255:Adenosine Triphosphate; D003402:Creatine Kinase
Affinnaia modifikatsiia kreatinkinazy iz skeletnykh myshts krolika s pomoshch'iu gamma-(p-azidoanilid)-ATP.