Mitotic gene conversion induced in yeast by isoniazid. influence of a transition metal and of the physiological conditions of the cells.
Isoniazid (INH) induced mitotic gene conversion at the trp5 locus of strain D7 of Saccharomyces cerevisiae. A suggested mechanism involving autoxidation of INH and production of hydrogen peroxide is supported by the facts that (i) the presence of a transition metal, Mn(II), greatly enhanced the effect of INH, (ii) cells stored for a long time in the refrigerator are much more sensitive to INH + Mn(II) than fresh cells probably due to loss of catalase and/or peroxidase activity, (iii) presence of S9 mix during the treatment eliminated the effect of INH + Mn(II).
D000818:Animals; D001711:Biotransformation; D005785:Gene Conversion / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007538:Isoniazid / Q000494:pharmacology*; D008297:Male; D008345:Manganese / Q000494:pharmacology; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism; D008938:Mitosis / Q000187:drug effects; D009152:Mutagenicity Tests; D010544:Peroxidases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013997:Time Factors
D008345:Manganese; D006861:Hydrogen Peroxide; D010544:Peroxidases; D007538:Isoniazid