[Effect of preparation procedures on the calculation of average cell volumes from stereological data].
The shrinkage of peritoneal cell during the electron microscopical preparation was investigated by means of a special device. Independently of the procedure of fixation after critical point drying the cells exhibit only 54 to 59% of the original diameter (residual volume about 18%). The reduction of cell diameter after Epon embedding is about 25%, corresponding to a residual volume of 40%.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D003584:Cytological Techniques; D003890:Desiccation; D005404:Fixatives; D008214:Lymphocytes / Q000166:cytology*; D008264:Macrophages / Q000166:cytology*; D008297:Male; D051379:Mice; D008854:Microscopy, Electron
D005404:Fixatives
Einfluss der Präparation auf die Berechnung des mittleren Zellvolumens aus stereologischen Daten.