Inhibition of thiamine transport in baker's yeast by methylene blue.
Methylene blue was found to inhibit thiamine transport competitively (Ki = 0.63 microM) in baker's yeast. The dye was also effective in abolishing the growth inhibition of Saccharomyces cerevisiae by pyrithiamine which is known to be taken up by a common transport system for thiamine in yeast cells. A possible mechanism for the inhibition by methylene blue of the thiamine transport system in baker's yeast is discussed.
D001692:Biological Transport / Q000187:drug effects; D007700:Kinetics; D008751:Methylene Blue / Q000494:pharmacology*; D011745:Pyrithiamine / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D013831:Thiamine / Q000378:metabolism*
D011745:Pyrithiamine; D008751:Methylene Blue; D013831:Thiamine