Microelisa test for detecting antibodies to rinderpest virus antigens.
A microplate enzyme-linked immunosorbent assay (ELISA) was developed which detected antibodies to a soluble antigen prepared from sonicated rinderpest virus-infected cells. The ELISA detected titres of antibody to the virus in the sera of cattle 3 weeks after immunisation with tissue culture rinderpest virus vaccine which were similar to those detected by the virus neutralisation test. The ELISA test shows potential as a rapid and economic technique for screening large numbers of sera for antibody to rinderpest virus.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D007630:Kenya; D009500:Neutralization Tests; D012301:Rinderpest / Q000175:diagnosis*; D012302:Rinderpest virus / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary
D000914:Antibodies, Viral; D000956:Antigens, Viral