The use of various buffers in the preparation of human lymphocytes for SEM observation.
A study is presented of various buffers utilized in the preparation of human lymphocytes for scanning electron microscopy. Of nineteen different buffers tested, Hanks' balanced salt solution +0.04 mol sucrose appeared most adequate for satisfactory preservation of lymphocyte surface architecture.
D002021:Buffers; D002462:Cell Membrane / Q000648:ultrastructure*; D003584:Cytological Techniques; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D008871:Microvilli / Q000648:ultrastructure*; D013048:Specimen Handling / Q000379:methods
D002021:Buffers