title: [Shigella flexneri mutation giving rise to the appearance of fosfomycin-resistant avirulent forms with disordered carbohydrate utilization].
abstract: The present work analyzes pt44 mutation in Sh. flexneri resulting in the appearance of the following phenotypical properties: resistance to phosphomycin, avirulence, pleiotropic disturbances in carbohydrate utilization. The data provided by the biochemical and genetic analysis have indicated that pts44 mutation occupies the region between purC and ptsI loci on the chromosome of Sh. flexneri. Merodiploids containing the mutant allele pts44 and the plasmid including purC-ptsI-ptsH genes from E. coli K12 acquired the capacity for fermenting carbohydrates, but at the same time retained resistance to phosphomycin and avirulent properties. The presence of phosphoenol pyruvate-dependent carbohydrate phosphotransferase system in Sh. flexneri has been proved.
mesh_terms: D000900:Anti-Bacterial Agents / Q000037:antagonists & inhibitors*; D050260:Carbohydrate Metabolism; D003433:Crosses, Genetic; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics; D005578:Fosfomycin / Q000037:antagonists & inhibitors*; D009154:Mutation; D010641:Phenotype; D011815:R Factors; D012763:Shigella flexneri / Q000235:genetics* / Q000378:metabolism; D014774:Virulence
chemical_list: D000900:Anti-Bacterial Agents; D005578:Fosfomycin
vernacular_title: Mutatsiia u Shigella flexneri, vyzyvaiushchaia poiavlenie fosfomitsinrezistentnykh avirulentnykh form s narusheniiami v utilizatsii uglevodov.