Identification of a cell-surface protein involved in the binding site of Sindbis virus on human lymphoblastic cell lines using a heterobifunctional cross-linker.
A cell-surface protein of molecular weight 90000 was identified as the major protein located at or near the binding site of Sindbis virus on the lymphoblastic cell lines JY and Daudi. This Sindbis-virus-binding protein was detected by cross-linking Sindbis virus, modified with 4-[6-formyl-3-azidophenoxy]butyrimidate, to the cell surface. The applicability of this compound in the identification of specific cell-surface proteins was demonstrated using a monoclonal antibody reactive with beta 2-microglobulin, a protein which is associated with the human histocompatibility antigens (HLA-A, HLA-B, HLA-C).
D001345:Autoradiography; D001665:Binding Sites; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D012845:Sindbis Virus / Q000378:metabolism*; D013499:Surface Properties; D001613:beta 2-Microglobulin / Q000276:immunology
D002352:Carrier Proteins; D003432:Cross-Linking Reagents; D008565:Membrane Proteins; D001613:beta 2-Microglobulin