New type of antibody-enzyme conjugate which specifically kills Candida albicans.
A new type of antibody-enzyme conjugate was made, and its possible application to Candida infection was studied. Both lactoperoxidase and xanthine oxidase were conjugated to specific antibody against Candida albicans. In vitro microbiocidal activity of the new antibody-enzyme conjugate, when incubated together with xanthine and minute amount of halides, showed a remarkable level of candidacidal ability. When the new antibody-enzyme conjugate was given to Candida-infected mice, followed by injecting xanthine and a minute amount of halides, about 50% of these heavily infected mice survived, whereas all control nontreated mice died. These data suggest that the further eleboration of this new antibody-enzyme conjugate might lead us to improve our therapeutic methods of clinical medicine.
D000818:Animals; D000908:Antibodies, Fungal / Q000008:administration & dosage*; D002176:Candida albicans / Q000254:growth & development* / Q000276:immunology; D002177:Candidiasis / Q000628:therapy*; D007454:Iodides / Q000627:therapeutic use; D007784:Lactoperoxidase / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010544:Peroxidases / Q000627:therapeutic use*; D014969:Xanthine Oxidase / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D014970:Xanthines / Q000627:therapeutic use
D000908:Antibodies, Fungal; D007454:Iodides; D014970:Xanthines; D007784:Lactoperoxidase; D010544:Peroxidases; D014969:Xanthine Oxidase