Immunogenicity of Staphylococcus aureus delta-toxin.
Studies were conducted to determine the immunogenicity of purified Staphylococcus aureus delta-toxin. Rabbits and guinea pigs immunized with delta-toxin incorporated into a multiple antibody, whereas animals given toxin in saline or toxin in saline with Tween 80 did not produce antibody. The immunoglobulin G (IgG) fraction isolated by chromatography on protein A-Sepharose was examined for the presence of anti-delta-toxin antibody by immunoelectrophoresis, immunodiffusion, quantitative precipitation tests, affinity chromatography, and toxin neutralization tests. Although delta-toxin-specific IgG precipitated the toxin in agar gels, the antibody did not neutralize the toxin's hemolytic activity. Delta-toxin binding to human erythrocyte membranes was demonstrated by indirect immunofluorescent staining of toxin-treated erythrocytes.
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000992:Antitoxins; D001427:Bacterial Toxins / Q000276:immunology* / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007158:Immunologic Techniques; D011485:Protein Binding; D013211:Staphylococcus aureus / Q000276:immunology*
D000907:Antibodies, Bacterial; D000992:Antitoxins; D001427:Bacterial Toxins; D007074:Immunoglobulin G; C025971:staphylococcal delta toxin