Insulin stimulates synthesis of soluble proteins in isolated rat hepatocytes.
The incorporation of [3H]leucine into soluble cellular protein was measured in isolated hepatocytes at extracellular leucine concentrations ranging from 0.15 to 20.0 mM. Insulin caused a 12--15% stimulation of [3H]leucine incorporation in the presence of high extracellular leucine concentrations. It is concluded that insulin causes a small but significant increase in the rate of hepatic protein synthesis.
D000818:Animals; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D014176:Protein Biosynthesis; D051381:Rats; D012995:Solubility; D013268:Stimulation, Chemical
D007328:Insulin; D007930:Leucine