Increased prostacyclin release from perfused hearts of acutely diabetic rats.
The release of prostacyclin and PGE2 from the isolated perfused hearts of acutely diabetic (streptozotocin 100 mg/kg) rats was studied and compared with hearts from control animals. Prostacyclin and PGE2 were measured by a differential bioassay technique. No basal release of either prostaglandin ws detected. However, after addition of arachidonic acid, a dose dependent release of prostacyclin and PGE2 was noted. Prostacyclin was identified as the major prostaglandin. Release of prostacyclin and PGE2 from acutely diabetic rat hearts was increased 2-3 times compared to control hearts. No release of prostaglandin endoperoxides was observed in either group of hearts.
D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology; D001681:Biological Assay; D003326:Coronary Circulation / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D011464:Epoprostenol / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010477:Perfusion; D011453:Prostaglandins / Q000378:metabolism*; D011458:Prostaglandins E / Q000378:metabolism*; D051381:Rats
D001095:Arachidonic Acids; D011453:Prostaglandins; D011458:Prostaglandins E; D011464:Epoprostenol; D007213:Indomethacin