Cell aggregate suspension culture for large-scale production of biomolecules.
A method is described for the rapid reversible conversion of a number of continuous cell lines from anchorage-dependent growth to growth as aggregates of cells in suspension culture. Employing this technique, an inoculum of three 75-cm2 flasks of BALB/c SV3T3 cells was grown to 60 liters of aggregate suspension in 14 days. This yielded 120 ml of packed cells or 9.1 x 10(10) cells. Similar results were obtained for other cell lines. Biomolecules such as migration-inhibition factor (MIF) and plasminogen activator were produced from these cultures.
D000818:Animals; D002449:Cell Aggregation; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D003470:Culture Media; D003584:Cytological Techniques; D004247:DNA / Q000378:metabolism; D008222:Lymphokines / Q000096:biosynthesis*; D051379:Mice; D010960:Plasminogen Activators / Q000096:biosynthesis*
D003470:Culture Media; D008222:Lymphokines; D004247:DNA; D010960:Plasminogen Activators