title: A method for studying the effect of inhibitors on the development of the isolated gonidia of Volvox tertius.
abstract: During asexual reproduction in Volvox tertius, a single cell, the gonidium, contained within the parental spheroid, undergoes enlargement followed by multiple cleavages. A method has been developed for the isolation and maintenance of gonidia in vitro in paraffin chambers. In these chambers development of the gonidium to a multicellular spheroid and subsequent inversion was apparently normal. The paraffin chambers allowed the monitoring of the effects of inhibitors on development without the possible interference of the parental spheroid. Enlargement and division of isolated gonidia was prevented by actinomycin D, cycloheximide and streptomycin. Division but not enlargement was inhibited by 5-fluorouracil, mitomycin C and ethidium bromide. Colchicine at high concentrations prevented inversion, as did streptomycin, cycloheximide and actinomycin D.
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000460:Chlorophyta / Q000187:drug effects / Q000254:growth & development*; D006131:Growth Inhibitors / Q000494:pharmacology*; D008828:Microbiological Techniques
chemical_list: D000900:Anti-Bacterial Agents; D006131:Growth Inhibitors