Insulin secretin of rat pancreatic islets incubated with autologous or heterologous serum.
Isolated rat pancreatic islets were incubated with either rat or human serum. Undiluted serum of both rat and man provided a strong insulin release, probably caused by a leakage of damaged B-cells. Heat inactivation of the serum only partially reversed this effect. Serum, which was diluted by 50% with buffer, had a much smaller cytotoxic activity. The effect on insulin leakage was further depressed when the complement system of the serum was inactivated by heat or zymosan treatment or storage of the serum before use. When rat islets were incubated in rat or human serum pretreated in this way, the glucose effect on insulin secretion could be determined.
D000818:Animals; D001769:Blood; D001800:Blood Specimen Collection; D002478:Cells, Cultured; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D007700:Kinetics; D051381:Rats; D013045:Species Specificity
D007328:Insulin