Metabolism of prostacyclin in Cynomolgus monkey.
The metabolism of prostacyclin (PGI2) in vivo was investigated in Cynomolgus monkey. Following intravenous infusion of 11-[3H]-PGI2 for three days, pooled urine was extracted with Amberlite XAD-2, then chromatographed and purified by Sephadex LH-20, and reverse phase column chromatography. Radioactive fractions were converted to appropriate derivatives for identification by gas chromatography mass spectrometry. Twelve metabolites were characterized, the major of which was 6-keto-PGF1 alpha, accounting for 13% of the urinary radioactivity. The metabolic pathways are similar to those observed earlier in the rat. The excretion of substantial amounts of unchanged 6-keto-PGF1 alpha indicated that the monkey was not able to metabolize PGI2 as avidly as the rat.
D015121:6-Ketoprostaglandin F1 alpha; D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D011464:Epoprostenol / Q000494:pharmacology / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D008251:Macaca / Q000652:urine*; D008252:Macaca fascicularis / Q000652:urine*; D008297:Male; D011453:Prostaglandins / Q000652:urine*; D011460:Prostaglandins F / Q000652:urine
D011453:Prostaglandins; D011460:Prostaglandins F; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol