Immunohistochemical evidence for a plasma membrane localization of dopamine-beta-hydroxylase in the mouse neuroblastoma.
Dopamine-beta-hydroxylase (D beta H), a glycoprotein enzyme which converts dopamine into noradrenaline, was purified from C1300 mouse neuroblastoma and used to raise antibodies in rabbits. Using an indirect immunofluorescence technique the cellular localization of D beta H in C1300 mouse neuroblastoma was compared with that of the superior cervical ganglion. C1300 neuroblastoma D beta H was found to be predominantly localized in the plasma membrane, in contrast to its intracellular localization in the superior cervical ganglion of A/J mice. At least part of the enzyme was found to be associated with the external side of the plasma membrane.
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D004299:Dopamine beta-Hydroxylase / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D051379:Mice; D009374:Neoplasms, Experimental / Q000201:enzymology / Q000648:ultrastructure; D009447:Neuroblastoma / Q000201:enzymology* / Q000648:ultrastructure
D004299:Dopamine beta-Hydroxylase