[Interactions of yeast hexokinase with ATP and AMP 4-(N-2-chloroethyl-N-methylamino)benzylamidates].
The interactions of ATP and AMP 4-(N-2-chloroethyl-N-methylamino)-benzylamidates with yeast hexokinase were studied. It was found that the ATP analog does not interact with hexokinase. The AMP analog irreversibly and almost completely inactivates hexokinse. A strong protective effect is exerted by MgATP. The value of apparent Ki for the AMP analog is equal to 2.10(4) M. The existence of affinity modification is postulated.
D000249:Adenosine Monophosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D006593:Hexokinase / Q000378:metabolism*; D007700:Kinetics; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013329:Structure-Activity Relationship
C026203:AMP-4-(N-2-chloroethyl-N-methylamino)benzylamidate; D000249:Adenosine Monophosphate; C025165:ATP-gamma-4-(N-2-chloroethyl-N-methylamino)benzylamidate; D000255:Adenosine Triphosphate; D006593:Hexokinase
Vzaimodeĭstvie 4-(N-2-khloretil-N-metilamino)-benzilamidov ATP i AMP s drozhzhevoĭ geksokinazoĭ.