title: A plasmid DNA primase active in discontinuous bacterial DNA replication.
abstract: A DNA primase encoded by an IncI alpha plasmid promotes efficient DNA replication in a primase-defective mutant of Escherichia coli. This finding implies that the plasmid enzyme can prime discontinuous DNA synthesis of the bacterial chromosome. The plasmid gene encodes two large, antigenically related proteins which differ from E. coli primase.
mesh_terms: D003086:Bacteriocin Plasmids; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D019915:DNA Primase; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004274:DNA, Recombinant / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D009154:Mutation; D010957:Plasmids; D012316:RNA Nucleotidyltransferases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D013489:Suppression, Genetic
chemical_list: D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004247:DNA; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases