Antigen expression by somatic cell hybrids of a murine embryonal carcinoma cell with thymocytes and L cells.
Thymocyte x PCC4azaR (a murine embryonal carcinoma cell) hybrid cells resembled PCC4azaR. They continued to express two embryonic antigens (SSEA-1 and 3C4-10) found on PCC4azaR, but did not express the thymocyte antigens Thy-1 and Ly-2. However, H-2Kk of thymocyte origin continued to be expressed, while H-2Db of PCC4azaR origin was not reexpressed. This is consistent with H-2 expression being subject to cis control. In syngeneic hosts these hybrids formed tumors containing stem cells and differentiated derivatives. In contrast, an L-cell x PCC4azaR hybrid resembled the L-cell parent, did not express SSEA-1 and 3C4-10, but did express both H-2Kk and H-2Db. In syngeneic hosts this hybrid formed undifferentiated sarcomas.
D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics; D002460:Cell Line; D006183:H-2 Antigens / Q000235:genetics*; D006822:Hybrid Cells / Q000276:immunology*; D007163:Immunosorbent Techniques; D007621:Karyotyping; D007739:L Cells; D051379:Mice; D008809:Mice, Inbred C3H; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology; D013724:Teratoma / Q000235:genetics*
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D006183:H-2 Antigens