Triazine dyes, a new class of affinity labels for nucleotide-dependent enzymes.
A number of reactive dichlorotriazine dyes specifically and irreversibly inactivate pig heart lactate dehydrogenase, yeast glucose 6-phosphate dehydrogenase and yeast hexokinase at sites competitive with NAD+, NADP+, and ATP respectively. Monochlorotriazine dyes, including Cibacron Blue F3G-A, do not inactivate lactate dehydrogenase but display high affinity and thus inhibit the inactivation by dichlorotriazine dyes. These data are interpreted in terms of the ability of nucleotide-binding enzymes to bind polysulphonated aromatic chromophores.
D000345:Affinity Labels; D000818:Animals; D001665:Binding Sites; D004396:Coloring Agents; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors; D006593:Hexokinase / Q000037:antagonists & inhibitors; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors*; D009206:Myocardium / Q000201:enzymology; D009243:NAD / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013552:Swine; D014227:Triazines
D000345:Affinity Labels; D004396:Coloring Agents; D014227:Triazines; D009243:NAD; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase