Intracellular distribution of nerve growth factor in rat pheochromocytoma PC12 cells: evidence for a perinuclear and intranuclear location.
The distribution of nerve growth factor (NGF) in rat pheochromocytoma cells (clone PC12) has been studied with two different techniques: immunofluorescence and autoradiography. It was found that NGF is progressively internalized in the cytoplasmic compartment and eventually accumulates in the form of discrete dots around the nucleus. A fraction of the internalized NGF appears within the nucleoplasm, often contiguous with the nucleolus. It is suggested that cytoplasmic and perinuclear NGF may be be in contact with a pool of tubulin or actin-like proteins in their soluble or organized form and play a key role in the process of arrest of division and neurite growth.
D000199:Actins / Q000378:metabolism; D001345:Autoradiography; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D009374:Neoplasms, Experimental / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism*; D010673:Pheochromocytoma / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism
D000199:Actins; D009414:Nerve Growth Factors; D014404:Tubulin