[Comparative morphological and insulin-producing characteristics of cellular cultures of the islands of Langerhans in experimental animals].
The pancreas from cattle and pigs (5--6-month-old), newborn rats, and guinea pits (1--1.5-month-old) was subjected to combined with trypsin and Wersen solutions and collagenase. Morphological changes and the insulin-producing activity of the primary cultures of the islet cells from these pancreas were studied. B- and A-cells were identified histologically. Concentration of insulin produced in the culture medium was determined immunoradiologically. The culture studied proved to preserve the morphological and functional differentiation for 4 to 5 weeks from the growth onset; their morphological changes during growth were similar. The level of the insulin produced depended on the cell composition and functional state of the culture. Increased glucse concentration (300 mg %) stimulated insulin secretion.
D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D005333:Fetus / Q000166:cytology; D006168:Guinea Pigs; D007328:Insulin / Q000096:biosynthesis* / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D013045:Species Specificity; D013552:Swine
D007328:Insulin
Sravnitel'naia morfologicheskaia i insulinprodutsiruiushchaia kharakteristika kletochnykh kul'tur ostrovkov Langergansa éksperimental'nykh zhivotnykh.