Block in insulin release from column-perifused pancreatic beta-cells induced by islet cell surface antibodies and complement.
Dispersed rat pancreatic islet cells were mixed into a short column of Bio-Gel P-2 polyacrylamide beads and perifused with an antiserum containing islet cell surface antibodies. The release of radioactive chromium from prelabeled cells, as a measure of cell membrane permeability, was not affected by cell surface antibodies alone, but increased dramatically in the presence of complement. While there was an eightfold increase in glucose-stimulated insulin release from beta-cells exposed to control serum and complement, insulin release was completely blocked from beta-cells exposed to islet-cell-specific antibodies and complement. These findings suggest that islet cell surface antibodies can mediate complement-dependent cytotoxicity.
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002463:Cell Membrane Permeability; D003165:Complement System Proteins / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000276:immunology* / Q000378:metabolism; D051381:Rats
D000906:Antibodies; D007328:Insulin; D003165:Complement System Proteins