Enumeration of haemopoietic progenitors (CFU-C and BFU-E) in liquid microculture using a limiting dilution analysis.
In order to overcome the difficulty of scoring multicentric colonies of haemopoietic progenitor cells, cultures in 10 microliter of liquid medium were grown. Plated at three different cell concentrations, progenitor cells (CFU-C or BFU-E) are not present in all wells. Their presence is easily scored in each well of microculture 7 days after incubation. A limiting dilution analysis allows one to obtain accurate quantification.
D000818:Animals; D002452:Cell Count; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004906:Erythrocyte Count; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D007201:Indicator Dilution Techniques; D008722:Methods; D051379:Mice