Monolayer destruction by leukocytes from patients with multiple sclerosis.
Separated lymphoid cells from patients suffering from multiple sclerosis (MS) were co-cultivated with various cell lines. Over 80% of such co-cultivations showed destruction of the tissue-culture monolayers, whereas less than 5% of "normal" blood co-cultivation behaved in the same manner. Because of the possible involvement of virus in the aetiology of MS, many positive co-cultivations were 1) examined electron-microscopically, but no virus particles were seen; and 2) tested for measles and herpes viruses using immunofluorescent techniques, but these also proved negative. Leukocytes from stroke patients showed monolayer destruction in about 50% of cases. Granulocyte contamination was high in the stroke blood samples. Reduction of granulocyte numbers to "normal" levels completely abrogated the effect in the stroke samples, but had no effect on the MS co-cultivations. Monolayer destruction by MS leukocytes also appeared not to be due to lymphotoxin.
D002460:Cell Line; D002561:Cerebrovascular Disorders / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology*; D009103:Multiple Sclerosis / Q000276:immunology*