Direct inhibitory effect of GnRH on androgen secretion by ovarian interstitial cells.
We report here 1) that GnRH and a potent GnRH agonist inhibit basal and LH stimulated ovarian androgen biosynthesis in vitro, 2) that the inhibitory effects of GnRH are dose-dependent and completely inhibited by concomitant treatment with a GnRH antagonist and 3) that the GnRH action is very rapid (t1/2 = 10 min) and persists after thorough washing of the cells. These data suggest that GnRH acts at a stereospecific binding site on ovarian theca and/or interstitial cells (probably the theca cells) to rapidly and perhaps irreversibly inhibit androgen biosynthesis.
D000728:Androgens / Q000378:metabolism*; D000818:Animals; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D007016:Hypophysectomy; D007700:Kinetics; D007986:Luteinizing Hormone / Q000494:pharmacology; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D051381:Rats
D000728:Androgens; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone