[Determination of the MSF (macrophage slowing factor) release by phytohemagglutinin using the macrophage electrophoretic mobility test (MEM test). I. Dependency of release on incubation time, phytohemagglutinin and cell concentration].
Under the influence of phytohaemagglutinin (PHA) lymphocytes of the peripheral blood from healthy persons and patients without immunodeficiencies produce a lymphokine called macrophage slowing factor (MSF). Kinetic studies of MSF production according to time-dependence, dose-response-effect and lymphocyte concentration revealed the optimal test conditions: 90 min resp. 4 h incubation time, 1 microliter and 5 microliter PHA per test and 10(6) lymphocytes per test. These conditions provided a decrease of 11.0 +/- 4.1% of macrophage mobility in the electric field. The PHA-model can be used as a positive control for the MEM-test. Moreover the PHA-model serves as a test for immunological reactivity.
D002478:Cells, Cultured; D006801:Humans; D007958:Leukocyte Count; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis*; D008264:Macrophages / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013997:Time Factors
D008263:Macrophage Migration-Inhibitory Factors; D010835:Phytohemagglutinins
Nachweis der Freisetzung des MSF (macrophage slowing factor) durch Phytohämagglutinin mit dem Makrophagen-Elektrophorese-Mobilitäts-Test (MEM-Test). I. Abhängigkeit der Freisetzung von der Inkubationszeit, der Phytohämagglutinin-Konzentration und der Zellkonzentration.