Use of protein A bearing Staphylococcus aureus in enzyme immunoassay (EIA).
Bovine serum albumin labeled with alkaline phosphatase and antibody have been employed as a model to determine if the use of Protein A bearing Staphylococcus aureus Cowan I strain (SACI) bacteria could be extended to enzyme immunoassay (EIA). SACI do not activate "per se" the enzyme substrate and bind aspectifically minimum amount of enzyme labeled antigen. Experimental conditions are described for the use of SACI both in macro and micro EIA assay which allows the processing of numerous samples with minimum handling. The sensitivity of the EIA is comparable with radioassy (EIA 2ng-RIA 4ng) which uses SACI in place of second antibody. The inhibition test can be performed in 4 hours time. These results suggest that the stability of SACI when combined with that of enzyme labeled antigens can widen the use of EIA, both for investigative and clinical studies.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000936:Antigen-Antibody Complex; D007124:Immunoenzyme Techniques; D013205:Staphylococcal Protein A / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*
D000936:Antigen-Antibody Complex; D013205:Staphylococcal Protein A; D000469:Alkaline Phosphatase