title: Purification of cytoplasmic antigens from the mycelial phase of Candida albicans: possible advantages of its use in Candida serology.
abstract: The serology of candidiasis is complicated by the use of poorly defined antigens. Total extracts of the yeast phase have been commonly used as "cytoplasmic' antigen, without regard to the significant amounts of carbohydrate that may contaminate such preparations. This is particularly true in the case of commercially available antigens that have been used as cytoplasmic antigens but actually are richer in carbohydrate than in protein. Affinity chromatography in concanavalin A - Sepharose provides a simple procedure to separate carbohydrates, mainly mannan, from protein antigens in whole Candida extracts. By using mannan-poor antigens, the specificity of serological reactions can be increased considerably, since both the positive reactions seen in a-symptomatic donors and the cross-reactions seen in patients infected with other fungi are due to anti-mannan antibodies. In contrast, both anti-mannan and anti-cytoplasmic antigen antibodies can be detected in patients suspected of systemic candidiasis. On the other hand, absolute specificity may never be achieved for systemic candidiasis. We have found antibodies against cytoplasmic antigen in a patient allergic to C. albicans, in whom the microorganism was isolated from fecal material. It appears that, under favorable conditions, mucosal sensitization may also trigger as systemic reaction directed against both mannan and cytoplasmic antigens.
mesh_terms: D000946:Antigens, Fungal / Q000032:analysis / Q000302:isolation & purification*; D002176:Candida albicans / Q000276:immunology*; D002177:Candidiasis / Q000175:diagnosis; D002241:Carbohydrates / Q000032:analysis; D011233:Precipitin Tests; D012698:Serologic Tests
chemical_list: D000946:Antigens, Fungal; D002241:Carbohydrates