Flow microfluorometric analysis of alloantigen expression during T cell development.
The fluorescence-activated cell sorter (FACS) was used to examine the expression of several alloantigens on T cells: Thy-1, Lyt-1.1, Lyt-2.1, Ly-5, Ly-6 and Ly-7. Antibodies secreted by monoclonal cell lines were used to characterize the Thy-1:2, Lyt-1.1 and Lyt-2.1 antigenic determinants, whereas Ly-5.1, Ly-6.2 and Ly-7.2 determinants were identified with alloantisera raised by conventional hyperimmunization. Using indirect immunofluorescence with fluorescein isothiocyanate-conjugated reagents, the profiles obtained with the FACS demonstrated that: (a) the expression of Thy-1 is greater on normal thymocytes than on cortisone-resistant thymocytes (CRT), lymph node and spleen cells; (b) in contrast to Thy-1, the expression of Ly-1 on normal thymocytes is less than on CRT, lymph node and spleen cells; (c) about 90% of normal thymocytes abut less than 50% of CRT, spleen and lympho node T cells are Lyt-2+ and (d) although weakly expressed on normal thymocytes, the amount of Ly-6 and Ly-7 present on peripheral T cells was considerably increased. The significance of these findings and the potential for further analysis of T cell developmental pathways is discussed.
D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation; D003348:Cortisone / Q000494:pharmacology; D007106:Immune Sera / Q000494:pharmacology; D007158:Immunologic Techniques; D007519:Isoantigens / Q000145:classification / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D011817:Rabbits; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000145:classification / Q000166:cytology*
D007106:Immune Sera; D007519:Isoantigens; D003348:Cortisone