[Evaluation of 2 methods for detecting anti-DNA antibodies in collagen disease].
An immunofluorescent technique using Crithidia luciliae (CL) has been developed to detect antibodies against native DNA, type them and determine their complement-fixing capacity. CL possess a kinetoplast containing double-stranded DNA that is not bound to histones: they are therefore a substrate well suited to the determination of antibodies against native DNA. When compared with a radioimmunoassay the technique displayed the same specificity and sensitivity. However, the correlation between clinical symptoms of SLE and anti-DNA antibody titer was better with the CL method than with radioimmunoassay. Determination of complement-fixing capacity and the type of immunoglobulins did not provide useful information. The CL method proved to be reliable, rapid and cheap.
D000328:Adult; D000974:Antibodies, Antinuclear / Q000032:analysis*; D003095:Collagen Diseases / Q000276:immunology*; D003168:Complement Fixation Tests; D003421:Crithidia / Q000276:immunology; D004247:DNA / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay
D000974:Antibodies, Antinuclear; D004247:DNA
Evaluation de deux méthodes de détection des anticorps anti-ADN natif dans les maladies du collagène.