Heteroimmunization with isolated human ova.
Rabbit antiserum raised against isolated human ova produced an intense immunofluorescence and a precipitate on the outer surface of human and porcine zonae pellucidae. The antiserum reacted with none of 22 human tissues nor with any of 4 body fluid components by immunofluorescence or immunodiffusion analysis, but the antiserum agglutinated AB erythrocytes after absorption with O erythrocytes. The anti-zona activity was achieved by higher titres for human than for porcine zonae. Immunofluorescence on porcine zonae was completely abolished by absorption with porcine ova, whereas a weak but definite fluorescence remained on human zonae. These findings indicate that the human zona pellucida consists of at least three distinct components; (1) a specific antigen(s) shared by human and porcine zonae, (2) an antigen(s) specific to human zonae, and (3) a non-specific antigen(s) associated with the blood group substances.
D000328:Adult; D000818:Animals; D000941:Antigens / Q000032:analysis; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D007163:Immunosorbent Techniques; D008875:Middle Aged; D010063:Ovum / Q000276:immunology*; D011817:Rabbits; D013552:Swine; D015044:Zona Pellucida / Q000276:immunology*
D000941:Antigens; D007106:Immune Sera