title: Nucleotide sequence of ilvGEDA operon attenuator region of Escherichia coli.
abstract: The nucleotide sequence of the DNA thought to contain the control region for the ilvGEDA operon in Escherichia coli has been determined by the Maxam-Gilbert procedure. The sequence includes a region that, upon transcription, would yield a leader transcript specifying a peptide 32 residues long. This putative peptide would contain four leucine, five isoleucine, and six valine residues. A model is proposed that correlates the multivalent control of the ilvGEDA operon with the extent to which this leader transcript is translated. In vitro transcription experiments yielded a transcript of about 183 nucelotides, compatible with the predictions of the model.
mesh_terms: D000597:Amino Acids, Branched-Chain / Q000235:genetics*; D003062:Codon; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005809:Genes, Regulator; D009690:Nucleic Acid Conformation; D009876:Operon; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000235:genetics; D012270:Ribosomes / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D000597:Amino Acids, Branched-Chain; D003062:Codon; D004269:DNA, Bacterial; D012343:RNA, Transfer