Differentiation of mycoplasmatales from bacterial protoplast L-forms by assay for penicillin binding proteins.
Membrane proteins with the specific ability for binding penicillin with high affinity (penicillin binding proteins) were found to be present in two strains of the cell wall-less protoplast L-form of P. Mirabilis and were absent from different species of Mycoplasma and from Acholeplasma laidlawii. Thus, the assay for penicillin binding proteins appeared to be suitable for the differentiation of the cell wall-less procaryotes. The absence of penicillin binding proteins from the mycoplasmatales further confirmed the unrelatedness of this group to the bacteria.
D000128:Acholeplasma laidlawii / Q000032:analysis / Q000145:classification*; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000032:analysis*; D006602:Hexosyltransferases; D007740:L Forms / Q000032:analysis / Q000145:classification*; D002267:Muramoylpentapeptide Carboxypeptidase; D009174:Mycoplasma / Q000032:analysis / Q000145:classification*; D046915:Penicillin-Binding Proteins; D010406:Penicillins / Q000032:analysis*; D010458:Peptidyl Transferases; D011513:Proteus mirabilis / Q000032:analysis
D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D010406:Penicillins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase