A radioisotopic leukocyte adherence test.
A modification of the leukocyte adherence inhibition method is described which utilizes 51Cr-labeled blood mononuclear cells placed in microwells. The test is reproducible, objective, employs approximately 2,000 cells per well, and allows multiple replicates of several antigens. With the two antigens tested, SK-SD and PPD, both increases and decreases of leukocyte adherence are observed. Serum proteins are important in the medium and inactivated AB serum gave the best results. A good correlation was observed between this leukocyte adherence test and positivity of skin testing,
D002860:Chromium Radioisotopes; D006801:Humans; D007158:Immunologic Techniques; D007957:Leukocyte Adherence Inhibition Test; D012882:Skin Tests; D013299:Streptodornase and Streptokinase / Q000276:immunology; D014373:Tuberculin / Q000276:immunology
D002860:Chromium Radioisotopes; D014373:Tuberculin; D013299:Streptodornase and Streptokinase