title: Microtest procedure for isolation of Chlamydia trachomatis.
abstract: Management of patients potentially infected with Chlamydia trachomatis has been hampered by the cost and time required to perform Chlamydia cultures. To isolate C. trachomatis, we developed a microtiter method that exhibited equal sensitivity but less frequent contamination than our previously used vial-cover slip culture system. In addition, costs and technician time were substantially reduced with the microtest method. Subsequent studies showed that cycloheximide-treated cells were superior to 5-iodo-2-deoxyuridine-treated cells in the microtest method and that a subpassage significantly enhanced the sensitivity of the method. The microtest method appears to be a sensitive, rapid, and economical method for isolating C. trachomatis.
mesh_terms: D001431:Bacteriological Techniques; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000175:diagnosis; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D003513:Cycloheximide / Q000494:pharmacology; D005260:Female; D006801:Humans; D007065:Idoxuridine / Q000494:pharmacology; D008297:Male; D014521:Urethra / Q000382:microbiology
chemical_list: D003513:Cycloheximide; D007065:Idoxuridine