Analysis of wall antigens of Aspergillus fumigatus by two-dimensional immunoelectrophoresis.
Water-soluble and surface-located antigens from Aspergillus fumigatus mycelium were analysed by two-dimensional electrophoresis (2D IEP) with selected sera. A complex pattern was seen when a water-soluble fraction reacted with hyperimmune sera. Significantly fewer precipitin peaks were seen when wall-located antigens were used in the 2D IEP system; this made possible the detection of a small number of recurring antigen-antibody interactions when sera from patients with aspergilloma or allergic bronchopulmonary aspergillosis were analysed. Many of these recurring precipitates were affected by lectins with specificity for alpha-D-glucose, alpha-D-mannose or N-acetyl-D-glucosamine. All the antigens were susceptible to hydrolysis by pronase, confirming the glycoprotein composition of many of these macromolecules.
D000818:Animals; D000946:Antigens, Fungal / Q000032:analysis* / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D001232:Aspergillus fumigatus / Q000276:immunology*; D002473:Cell Wall / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D037102:Lectins / Q000276:immunology; D011817:Rabbits
D000946:Antigens, Fungal; D000954:Antigens, Surface; D006023:Glycoproteins; D037102:Lectins