[Production and characterization of a soluble ocrase-polyacrylamide preparation (author's transl)].
Using the carbodiimide procedure, ocrase, a proteinase isolated from Aspergillus ochraceus, has been bonded to a water-soluble acrylamide-acrylic acid copolymer and isolated by gel filtration. Binding to the high-molecular polymer had implications for the kinetics of the cleavage of the ethyl ester of benzoyl-L-arginine by this proteinase, and for the reaction of the latter with synthetic and natural inhibitors.
D000180:Acrylic Resins; D001230:Aspergillus / Q000201:enzymology; D004339:Drug Compounding; D005782:Gels; D007700:Kinetics; D010447:Peptide Hydrolases / Q000008:administration & dosage* / Q000378:metabolism; D011480:Protease Inhibitors; D012995:Solubility
D000180:Acrylic Resins; D005782:Gels; D011480:Protease Inhibitors; C016680:polyacrylamide gels; D010447:Peptide Hydrolases; C021901:ocrase
Herstellung und Charakterisierung eines löslichen Ocrase-Polyacrylamidpräparates.