[Changes in the transmembrane potential of developing loach embryos under the influence of insulin, inhibition of transcription and translation].
Results of continuous 7-8 hour registration of transmembrane potential (TMP) at the early stages of the loach (Misgurnus fossilis) development in normal conditions and in media with different insulin concentrations (the hormone with actinomycin D and cycloheximid and these antibiotics alone) are presented in this paper. The dependence of the absolute value and the mode of TMP changes in developing embryos upon insulin concentration has been established. It is shown that actinomycin D reduces the hormonal effect at the initial stages, while alone it does not cause any visible changes of TMP. Inhibition of translation with cycloheximid leads to sharp depolarizaton of the membrane and completely abolishes the periodicity of TMP oscillations, which in the norm corresponds to the time-course of the mitotic cycles during the synchronous cleavang of the blastomeres.
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004347:Drug Interactions; D004625:Embryo, Nonmammalian / Q000187:drug effects; D005399:Fishes / Q000196:embryology*; D007328:Insulin / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D008938:Mitosis; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
D007328:Insulin; D003609:Dactinomycin; D003513:Cycloheximide
Izmeneniia transmembrannogo potentsiala u razvivaiushchikhsia zarodysheĭ v'iuna pod vliianiem insulina, ingibirovaniia transkriptsii i transliatsii.