[Effect of plasmid R6K on expression of the temperature-sensitive mutation in gene dnaE of Escherichia coli K-12].
The multicopy, conjugative R6K plasmid is responsible for the increase in the number of temperature-resistant (Tr) clones formed by Escherichia coli K-12 E486 strain, carrying a ts mutation dnaE486 in the gene coding for DNA polymerase III. The effect observed is not due to the mutator action of R6K or a mutation in the plasmid and requires the intactness of the host recA function. Tr derivatives MG488 and MG492, isolated under non-permissive condition from the strain E486(R6K), still possess the ts allele dnaE486 in the chromosome. Both Tr derivatives are more resistant to UV-irradiation and the characterized with a different level of spontaneous and UV-induced mutagenesis as compared to the initial strain E486(R6K). The data obtained suggest that the plasmid R6K is involved in the metabolism of chromosomal DNA of the host.
D000483:Alleles; D004258:DNA Polymerase III / Q000235:genetics*; D004269:DNA, Bacterial / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D004794:Enzyme Repression; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D005796:Genes; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D013045:Species Specificity; D013696:Temperature; D014161:Transduction, Genetic; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase
Vliianie plazmidy R6K na proiavlenie temperaturchuvstvitel'noĭ mutatsii v gene dnaE u Escherichia coli K-12.