title: Organization and regulation of the ilvGEDA operon in Salmonella typhimurium LT2.
abstract: A total of 102 isoleucine- and isoleucine-valine-requiring (ilv) mutants induced by insertion of the transposable element Tn10 have been classified to cistron by growth requirement, cross-feeding behavior, and enzyme assays. The mutations are in a polycistronic operon transcribed in the order ilvGEDA and in a monocistronic operon ilvC. Analysis of distal gene expression in these polar insertion mutants revealed the existence of two constitutive interval promoters, one preceding ilvE and the other preceding ilvD.
mesh_terms: D002874:Chromosome Mapping; D005786:Gene Expression Regulation; D005796:Genes; D007532:Isoleucine / Q000235:genetics*; D009154:Mutation; D009876:Operon; D012486:Salmonella typhimurium / Q000235:genetics*; D014633:Valine / Q000235:genetics*
chemical_list: D007532:Isoleucine; D014633:Valine