A radioenzymatic method for determination of normetanephrine in blood plasma.
A radioenzymatic assay for determination of normetanephrine in blood plasma is described. It was based on N-methylation of normetanephrine by phenylethanolamine-N-methyltransferase using S-adenosyl[methyl-3]methionine as the methyl donor. 2981 +/- 85 cpm (mean +/- s mean, n = 32) were obtained from 1 ng normetanephrine. Blank values corresponded to 27 +/- 3 pg (n = 7). Normetanephrine levels measured in the blood plasma of 12 persons ranged from 1.3 to 9.6 ng/ml.
D006801:Humans; D009647:Normetanephrine / Q000097:blood*; D010625:Phenylethanolamine N-Methyltransferase; D011865:Radioisotope Dilution Technique; D012436:S-Adenosylmethionine; D014316:Tritium
D009647:Normetanephrine; D014316:Tritium; D012436:S-Adenosylmethionine; D010625:Phenylethanolamine N-Methyltransferase