Bacterial adherence to eucaryotic cells: isolation of lymphocyte-binding mutants.
A procedure for obtaining bacterial mutants that bind to eucaryotic cells is described. This procedure takes advantage of the ability of the mutants to obtain a required nutrient from the eucaryotic cells. We used this procedure to isolate mutants of Escherichia coli that bind to mouse lymphocytes. We show that the mutants identify some immunoglobulin-bearing lymphocytes and some non-immunoglobulin-bearing lymphocytes.
D000818:Animals; D001402:B-Lymphocytes / Q000382:microbiology; D001431:Bacteriological Techniques; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D007074:Immunoglobulin G; D008214:Lymphocytes / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D013601:T-Lymphocytes / Q000382:microbiology
D007074:Immunoglobulin G