title: Peptones and calf serum as a replacement for human serum in the cultivation of Plasmodium falciparum.
abstract: Attempts were made to find an inexpensive, readily available substitute for human serum requirement for the continuous culture of erythrocytic stages of Plasmodium falciparum. We found that Neopeptone or Proteose-Peptone No. 3 added together with calf serum gave parasite growth rates comparable to, or surpassing, those obtained with human serum. However, first it was necessary to adapt the parasites by a gradual, stepwise reduction in the amount of human serum used, and a concomitant, stepwise increase in the substitutes added.
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001769:Blood; D002417:Cattle / Q000097:blood; D003470:Culture Media; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D010274:Parasitology / Q000379:methods*; D010461:Peptones / Q000494:pharmacology*; D010963:Plasmodium falciparum / Q000254:growth & development*; D013552:Swine / Q000097:blood
chemical_list: D003470:Culture Media; D010461:Peptones