[Penicillin amidase from E. coli. The kinetic and equilibrium parameters of the enzymatic hydrolysis of 7-phenylacetamidodesacetoxycephalosporanic acid catalyzed by an immobile enzyme].
The kinetics of 7-phenylacetamidodesacetoxycephalosporanic acid (7-PADCA) catalyzed by immobilized penicillinamidase was studied. The kinetic and equilibrium parameters of the reaction were determined by analysis of the kinetic curves of the reaction product accumulation. Inhibition of the enzymatic reaction by the substrate and hydrolysis products was studied. It was found that the Michaelis complex completely lost its activity after attachment of the substrate second molecule to it. The values of the Michaelis constants, catalytic constant and constants of inhibition by the substrate and reaction products were determined: Km = (9.3 +/- 1.1) . 10(-5) M, kcat = (65 +/- 5) c-1, Ks = (1.4 +/- 0.1) . 10(-2) M, K1 (FAA) = (2.5 +/- 0.3) . 10(-4) M, K1 (7-ADCA) = (1.4 +/- 0.1) . 10(-1) M. The diffusion effect in the kinetic reaction catalyzed by immobilized penicillinamidase is discussed. The values of the Thiele modulus and the actual value of Km were calculated.
D000581:Amidohydrolases / Q000378:metabolism*; D002384:Catalysis; D002511:Cephalosporins / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D006868:Hydrolysis; D007700:Kinetics; D008433:Mathematics; D010399:Penicillin Amidase / Q000378:metabolism*; D013379:Substrate Specificity
D002511:Cephalosporins; D004800:Enzymes, Immobilized; C013644:7-phenylacetamidodeacetoxycephalosporanic acid; D000581:Amidohydrolases; D010399:Penicillin Amidase
Penitsillinamidaza iz E. coli. Kineticheskie i ravnovesnye parametry fermentativnogo gidroliza 7-fenilatsetamidodezatsetoksitsefalosporanovoi kisloty, kataliziruemogo immobilizovannym fermentom.