Inactivation of yeast glucose-6-P dehydrogenase by aspirin.
Glucose-6-P dehydrogenase is irreversibly inactivated by treatment with Na salts of aspirin. Kinetic data show that 1 molecule of aspirin reacts with each active unit when the enzyme is inactivated. The rate of inactivation is enhanced with increasing pH but is reduced in the presence of glucose-6-P or NADP+. Na salicylate fails to inactivate the enzyme.
D001241:Aspirin / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009249:NADP; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D009249:NADP; D005954:Glucosephosphate Dehydrogenase; D001241:Aspirin