Growth of mammalian cell lines in a spinner culture.
Several mammalian cell lines, derived from embryonic, normal adult and malignant tissues were adapted for long-term growth in agitated fluid medium. A simple method of spinner culture is described, employing a portable magnetic stirrer. Adaptation of cells for growth in spinner culture took a relatively short time, usually a few weeks, depending on the type of cell line used. Characteristics of cell lines maintained in spinner culture are described in details.
D000220:Adaptation, Biological; D000818:Animals; D002460:Cell Line; D003584:Cytological Techniques; D051381:Rats