title: Specificity of chromatin transcription in vitro. Asymmetric transcription of the globin gene by Escherichia coli RNA polymerase.
abstract: The transcription of globin genes in mouse foetal liver chromatin and nuclei by exogenous Escherichia coli RNA polymerase is prone to artifacts due to RNA-dependent transcription of endogenous mRNA sequences. This is particularly evident when Mn2+ is used as divalent cation in the RNA transcription reaction. However, substitution or supplementation with Mg2+ eliminates this artifact and gives essentially asymmetric DNA-dependent transcription by the polymerase. In this paper we discuss a number of general criteria which can be applied to test the validity of specific gene transcription in vitro.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics*; D005914:Globins / Q000235:genetics*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008274:Magnesium / Q000502:physiology; D051379:Mice; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D005914:Globins; D012321:DNA-Directed RNA Polymerases; D008274:Magnesium