Primary culture of mouse mammary tumor epithelial cells embedded in collagen gels.
Mammary tumor epithelial cells from BALB/cfC3H mice were dispersely embedded inside the collagen gels in Ham's F-12 medium containing horse serum. A sustained cell growth leading to a 5- to 10-fold increase in cell number over initial level was observed in less than 2 weeks. The extent of this growth was found to be dependent on serum concentration. However, addition of various protein and steroid hormones, both singly and in combination, to low-serum-containing medium failed to achieve a comparable level of growth to that promoted by higher serum concentration. Mammary tumor cells can now be consistently propagated in primary culture.
D000305:Adrenal Cortex Hormones / Q000494:pharmacology; D000818:Animals; D001769:Blood; D002455:Cell Division; D002478:Cells, Cultured / Q000166:cytology*; D003094:Collagen; D003470:Culture Media; D003584:Cytological Techniques; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004848:Epithelium / Q000473:pathology; D012739:Gonadal Steroid Hormones / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008325:Mammary Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D011388:Prolactin / Q000494:pharmacology
D000305:Adrenal Cortex Hormones; D003470:Culture Media; D004273:DNA, Neoplasm; D012739:Gonadal Steroid Hormones; D007328:Insulin; D011388:Prolactin; D003094:Collagen