A simple method for the preparation of cell cultures for ultrastructural investigation.
A method is described that allows investigation of cultured cells at the light or electron microscopical level without changing the culturing conditions or the in situ situation during dehydration or embedding. Fixed and dehydrated cell culture layers were cut into small pieces and separated from the plastic dish by adding propylene oxide. With this simple method we could obtain orientated sections of selected cell groups.
D000818:Animals; D001011:Aorta; D002417:Cattle; D002478:Cells, Cultured / Q000648:ultrastructure*; D003584:Cytological Techniques; D004727:Endothelium / Q000648:ultrastructure; D006801:Humans; D009130:Muscle, Smooth / Q000648:ultrastructure; D009131:Muscle, Smooth, Vascular / Q000648:ultrastructure; D011817:Rabbits; D051381:Rats; D014470:Umbilical Cord