Identification of tissue-specific nuclear antigens transferred to nitrocellulose from polyacrylamide gels.
Nonhistone protein antigens resolved by electrophoresis in sodium dodecyl sulfate were identified immunochemically after being transferred to nitrocellulose. Use of antiserum to dehistonized chromatin from Novikoff hepatoma revealed numerous protein antigens specific to the chromatin of Novikoff hepatoma in comparison to that of normal rat liver.
D000818:Animals; D002467:Cell Nucleus / Q000276:immunology*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007124:Immunoenzyme Techniques; D008099:Liver / Q000032:analysis* / Q000648:ultrastructure; D008114:Liver Neoplasms, Experimental / Q000032:analysis* / Q000648:ultrastructure; D008297:Male; D051381:Rats
D002868:Chromosomal Proteins, Non-Histone