Identification of canine lymphocyte populations by immunofluorescence surface marker analysis.
A T-cell-specific rabbit anti-dog thymocyte antiserum was prepared and used in an indirect fluorescent antibody technique to identify T lymphocytes. The antiserum was rendered specific by absorptions with normal dog red blood cells, lyophilized serum, and bone marrow cells. By the indirect immunofluorescence assay, 73.6 +/- 1.2 (SEM)% of the peripheral blood lymphocytes were identified as T lymphocytes. By direct immunofluorescence assay of surface membrane immunoglobulin-positive lymphocytes, 18.2 +/- 0.8% of the peripheral blood lymphocytes were identified as B lymphocytes. The two assays used in conjunction can thus identify over 90% of the peripheral blood lymphocytes as either T or B lymphocytes, with the remaining 8.2 +/- 1.0% as null cells.
D000818:Animals; D000918:Antibody Specificity; D000961:Antilymphocyte Serum; D001402:B-Lymphocytes / Q000145:classification; D004285:Dogs; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D011817:Rabbits / Q000276:immunology; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000145:classification*; D013950:Thymus Gland / Q000166:cytology
D000961:Antilymphocyte Serum