Expression of endogenous retroviral envelope antigen in Ig(+) B cells of immunized 15I5 X 71 chickens.
In a previous study (1), we observed that antigen cross-reactive with structural protein of the endogenous retrovirus RAV-O is expressed in splenic Ig(+) B lymphocytes of immunized 15I5 X 71 chickens. The present experiments were undertaken to determine the relationship of this antigen to viral gag- and env-coded antigenic specificities. As analyzed by immunofluorescence with several antisera, group-specific determinants of the gp85 envelope glycoprotein as well as viral-related type E-specific determinants were detectable in islands of Ig(+) splenocytes; antigenic determinants of viral gag protein were not detected in the splenic islands. These observations indicated that antigen cross-reactive with endogenous retroviral envelope glycoprotein is expressed in Ig(+) B cells.
D000042:Absorption; D000818:Animals; D000956:Antigens, Viral; D001402:B-Lymphocytes / Q000276:immunology*; D002645:Chickens / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006041:Goats; D007136:Immunoglobulins; D008297:Male; D011784:Quail; D011817:Rabbits; D012190:Retroviridae / Q000276:immunology*; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000276:immunology
D000956:Antigens, Viral; D007136:Immunoglobulins