Labelling of the cyclitol permease in Klebsiella aerogenes.
The protein component(s) of the cyclitol-transport system in Klebsiella aerogenes has been labelled by using three different procedures. One method is based on differential alkylation with N-ethylmaleimide, and another on incorporation of amino acids during the induction process. A protein of mol.wt. 34 000 was labelled by both procedures; by alkylation with N-ethylmaleimide two other proteins of mol.wts. 55 000 and 67 000 were also labelled. The third uses diazotized [35S]sulphanilic acid after protection by substrate and the comparison of labelling of induced cells with non-induced cells; the label was also concentrated in a mol.wt.-33 000 peak. The labelled protein is, from the evidence, the cyclitol carrier.
D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D003979:Diazonium Compounds / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005033:Ethylmaleimide / Q000494:pharmacology; D007294:Inositol / Q000494:pharmacology; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism*; D008722:Methods; D013425:Sulfanilic Acids / Q000031:analogs & derivatives / Q000378:metabolism
D000596:Amino Acids; D001426:Bacterial Proteins; D003979:Diazonium Compounds; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D013425:Sulfanilic Acids; D007294:Inositol; D005033:Ethylmaleimide; C000469:diazobenzenesulfonic acid