title: [Affinity modification of Escherichia coli Dna-polymerase I by 4-(N-2-chloroethyl-N-methylamino)-benzyl-gamma-amides of dTTP and dATP].
abstract: The interaction of dTTP and dATP gamma-4-(N-2-chloroethyl-N-methylamino) benzylamidates with E. coli DNA-polymerase I were studied. dTTP and dATP gamma-4-(N-2-hydroxyethyl-N-methylamino) benzylamidates act as competitive inhibitors of DNA-polymerase I. Ki values for gamma-analogues of dTTP and dATP have been determined. Reactive dTTP and dATP derivatives are shown to be affinity reagent for this enzyme.
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives*; D055598:Chemical Phenomena; D002621:Chemistry; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors; D004259:DNA-Directed DNA Polymerase; D004926:Escherichia coli / Q000201:enzymology*; D013942:Thymine Nucleotides
chemical_list: D013942:Thymine Nucleotides; C025785:4-(N-(2-chloroethyl)-N-methylamino)benzyl-gamma-amide dTTP; C025786:4-(N-2-chloroethyl-N-methylamino)benzyl-gamma-amide dATP; D000255:Adenosine Triphosphate; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase
vernacular_title: Affinnaia modifikatsiia DNK-polimerazy I iz Escherichia coli 4-(N-2-chloroéthyl-N-methylamino)-benzyl-gamma-amidami dTTP i dATP.