Use of gene fusions to determine the orientation of gene phoA on the Escherichia coli chromosome.
We present genetic evidence which demonstrates that the phoA gene is transcribed in the clockwise direction on the Escherichia coli chromosome, in contrast to an earlier proposal. Our conclusion is based on analysis of various genetic fusions between the lac operon and the phoA gene.
D000469:Alkaline Phosphatase / Q000235:genetics*; D002876:Chromosomes, Bacterial; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D007763:Lac Operon; D009154:Mutation; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D000469:Alkaline Phosphatase