Structure of the peptidoglycan-associated lipoprotein (PAL) of the Proteus mirabilis outer membrane. I. Isolation and characterization of fatty acid-containing peptides from PAL.
In the outer membrane of P. mirabilis, a peptidoglycan-associated lipoprotein (PAL) of apparent molecular weight 18,000 is present as a major protein. A fatty acid-containing polypeptide (lipopolypeptide; LPP) was isolated by digestion of the purified PAL with trypsin in the presence of 0.05% SDS. It was composed of 31 amino acid residues, an unidentified compound[X], and ca. 3 fatty acid residues. A lipooligopeptide (LOP) was also isolated after further digestion of LPP with trypsin in the absence of SDS. LOP was composed of 4 amino acid residues (Asx, 2Ser, Lys), a compound[X], and ca. 3 fatty acid residues. The C-terminal amino acids of LPP and LOP were determined as arginine and lysine, respectively. On the other hand, the N-terminus of PAL, LPP, or LOP could not be identified by conventional N-terminal analysis of PAL, LPP, or LOP could not be identified by conventional N-terminal analysis, indicating that the N-terminus is probably masked. These results indicate that LPP and LOP are derived from the fatty-attached amino terminal region of PAL.
D000596:Amino Acids / Q000032:analysis; D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D005227:Fatty Acids / Q000032:analysis*; D008074:Lipoproteins / Q000032:analysis*; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification*; D010457:Peptidoglycan / Q000032:analysis*; D011509:Proteoglycans; D011513:Proteus mirabilis / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D012995:Solubility; D014357:Trypsin / Q000378:metabolism
D000596:Amino Acids; D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; C031163:ExcC protein, E coli; D005227:Fatty Acids; D008074:Lipoproteins; D010455:Peptides; D010457:Peptidoglycan; D011509:Proteoglycans; C069988:PplA protein, Legionella pneumophila; D012967:Sodium Dodecyl Sulfate; D014357:Trypsin