title: Growth characteristics of group A streptococci in a new chemically defined medium.
abstract: A new chemically defined medium for the growth of group A streptococci has been formulated. The advantages of this new medium over previously described defined media are: (i) rates of growth (i.e., doubling times) of 20 strains were comparable to the rates of growth in complex media; (ii) each strain grew to a higher culture density in the new defined medium than in complex media; (iii) transfer from complex media with small inocula was possible without any prior adaptation regimen; and (iv) the production of virulence factors (i.e., M protein and hyaluronic acid) and extracellular enzymes during growth in this new medium was comparable to that in complex media.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis; D003470:Culture Media; D003851:Deoxyribonucleases / Q000096:biosynthesis; D006820:Hyaluronic Acid / Q000096:biosynthesis; D007700:Kinetics; D013297:Streptococcus pyogenes / Q000254:growth & development* / Q000378:metabolism; D013301:Streptolysins / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D013301:Streptolysins; D006820:Hyaluronic Acid; D003851:Deoxyribonucleases