A possible functional relationship between microsomal aromatic aldehyde-ketone reductase and hexose-6-phosphate dehydrogenase.
Aromatic ketone reductase activity of microsomes showed a unique cofactor requirement: Addition of NADP and glucose-6-phosphate was as effective as that of an artificial NADPH generating system, whereas NADPH alone served as a cofactor less efficiently. Microsomal aromatic ketone reductase, purified partially from guinea pig liver microsomes after solubilization with Triton X-100, reduced 5 beta-dihydrotestosterone, aromatic aldehydes, and ketones with NADPH as a cofactor. However, addition of hexose-6-phosphate dehydrogenase, purified from the same source, as an NADPH generator produced about 2 times higher activity than that of yeast glucose-6-phosphate dehydrogenase or NADPH alone.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000631:Aminopyridines; D000818:Animals; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D003067:Coenzymes / Q000378:metabolism; D006168:Guinea Pigs; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009249:NADP / Q000378:metabolism; D013379:Substrate Specificity
D000631:Aminopyridines; D003067:Coenzymes; D009249:NADP; D000429:Alcohol Oxidoreductases; D002237:Carbohydrate Dehydrogenases; C020552:galactose-6-phosphate dehydrogenase