Separation of stages of Plasmodium falciparum-infected cells by means of a fluorescence-activated cell sorter.
Plasmodium falciparum parasites from long-term in vitro culture have been labeled with the DNA-binding dye Hoechst 33258. After labeling, parasitized cells have been successfully analyzed and sorted, using a fluorescence-activated cell sorter, into populations of uninfected, singly infected, and multiply infected cells.
D002469:Cell Separation / Q000379:methods*; D004912:Erythrocytes / Q000469:parasitology*; D005453:Fluorescence; D006801:Humans; D008288:Malaria / Q000469:parasitology*; D010963:Plasmodium falciparum / Q000254:growth & development