The effect of insulin on the electrophoretic mobility of rat hepatocytes.
The addition of insulin (10 microU) to a suspension of isolated hepatocytes in Krebs-Ringer bicarbonate solution, causes an increase in the negative electrophoretic mobility of the cells from - 1.68 micrometer sec-1 V-1 cm to 2.26 micrometer sec-1 V-1 cm. This observation supports the findings by other workers that the binding of insulin to its receptor leads to a marked change in the membrane.
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D004586:Electrophoresis; D007328:Insulin / Q000494:pharmacology*; D007526:Isoelectric Point; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D013997:Time Factors
D007328:Insulin; D003208:Concanavalin A