Radioimmunoassay of leucine-enkephalin-like substance in human and canine plasma.
Fasting human plasma immunoreactive leu-enkephalin (ir-leu-enkephalin) measured by radioimmunoassay averages 54+/-10 pg/ml. The method depends on acidification of plasma to protect against destruction of the peptide, adsorption to XAD-2 resin, extraction from the resin by aqueous methanol and concentration by evaporation. Plasma enkephalin in the dog increased from 13 to 56 pg/ml following insulin-induced hypoglycemia.
D000328:Adult; D000818:Animals; D003488:Cyanogen Bromide; D004285:Dogs; D004355:Drug Stability; D004723:Endorphins / Q000097:blood*; D004743:Enkephalin, Leucine; D004745:Enkephalins / Q000097:blood*; D005215:Fasting; D006801:Humans; D007003:Hypoglycemia / Q000097:blood / Q000139:chemically induced; D007328:Insulin; D008297:Male; D011863:Radioimmunoassay / Q000379:methods
D004723:Endorphins; D004745:Enkephalins; D007328:Insulin; D004743:Enkephalin, Leucine; D003488:Cyanogen Bromide