Destruction of articular cartilage by arthritic synovium in vitro: mechanism of breakdown and effect of indomethacin and prednisolone.
Arthritic synovial tissue when cultured with normal articular cartilage induces a breakdown of articular cartilage proteoglycans. No collagen breakdown occurs unless the macroglobulins are inactivated by pretreatment with acid. Proteoglycan breakdown is most likely due to the continuous secretion of hydrolases by the synovium. A proteolytic enzyme has been found in the culture medium which has a pH optimum around 7.6, and is Ca++ dependent. Both indomethacin and hydrocortisone are inhibitors of proteoglycan breakdown.
D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D001168:Arthritis / Q000473:pathology*; D001169:Arthritis, Experimental / Q000473:pathology*; D002118:Calcium / Q000494:pharmacology; D002358:Cartilage, Articular / Q000187:drug effects / Q000473:pathology*; D003470:Culture Media; D046508:Culture Techniques; D006854:Hydrocortisone / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007213:Indomethacin / Q000494:pharmacology*; D008297:Male; D009924:Organ Culture Techniques; D010447:Peptide Hydrolases / Q000378:metabolism; D011817:Rabbits; D013583:Synovial Membrane / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*
D000963:Antimetabolites; D003470:Culture Media; D010447:Peptide Hydrolases; D002118:Calcium; D006854:Hydrocortisone; D007213:Indomethacin