[Comparative features of methos of inducing auxotrophic mutants of cholera and nonagglutinating vibrios].
The effect of chemical mutagens (EI, NEU, NMU, NMG) on six El-Tor strains and eight NAG-vibryos was studied in order to obtain auxotrophic mutatns. The methods used showed the advantage of A. Adelberg and D. Mayers's method in the Yu. G. Suchkov's modification. It enables to get a greater number of mutants, and it differs from other methods in the simplicity in organization of experiments. The mutants obtained of El-Tor and NAC-vibryos differed from each other and from initial strains in their biochemical properties, agglutinability, phagolisability and culture requirements.
D001388:Aziridines / Q000494:pharmacology; D005038:Ethylnitrosourea / Q000494:pharmacology; D005821:Genetic Techniques; D008770:Methylnitrosourea / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D009604:Nitrosoguanidines / Q000494:pharmacology; D013045:Species Specificity; D014733:Vibrio / Q000235:genetics*; D014734:Vibrio cholerae / Q000235:genetics / Q000378:metabolism
D001388:Aziridines; D009153:Mutagens; D009604:Nitrosoguanidines; D008770:Methylnitrosourea; D005038:Ethylnitrosourea
Sravnitel'naia kharakteristika metodov induktsii auksotrofnykh mutantov kholernykh i neaggliutiniruiushchikhsia vibrionov.