title: Influence of complement on the chemiluminescent response of human leukocytes to immune complex.
abstract: Vaccine bacteria of Francisella tularensis were mixed with human serum containing specific antibodies against the same organism, and the mixture was incubated at 37 degrees C for 30 min. The mixture induced a two-peak chemiluminescent response in polymorphonuclear leukocytes. The initial peak was induced by a soluble agent, formed during incubation. The formation of this agent involved the activation of complement component C5. The second peak of the chemiluminescent response was induced by the opsonized bacteria; this peak was augmented by complement component C3, whereas C5 had no influence.
mesh_terms: D000936:Antigen-Antibody Complex; D001769:Blood; D003182:Complement C5 / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology*; D005604:Francisella tularensis / Q000276:immunology; D006801:Humans; D007114:Immunization; D007158:Immunologic Techniques; D008163:Luminescent Measurements; D009504:Neutrophils / Q000276:immunology*; D009895:Opsonin Proteins / Q000276:immunology*
chemical_list: D000936:Antigen-Antibody Complex; D003182:Complement C5; D009895:Opsonin Proteins; D003165:Complement System Proteins