Human plasma transforms prostacyclin (PGI2) into a platelet antiaggregatory substance which contracts isolated bovine coronary arteries.
Prostacyclin (PGI2) incubated in Human Platelet Rich Plasma (PRP); in Platelet Poor Plasma (PPP) or in Krebs-Ringer-Bicarbonate (KRB) during different periods of time on contractions of bovine coronary arteries and on the ADP platelet aggregative capacity of human PRP, were explored. It was documented that incubates in PRP or in PPP retain an antiaggregatory activity at higher levels and during a longer time than in KRB. On the other hand, PGI2 incubates in KRB exhibited only a relaxing activity on isolated bovine coronary arteries, whereas when incubated in PRP or in PPP presented a biphasic influence. The initial effects (evoked by incubates of 30 minutes) were distinctly relaxing but those obtained with later incubates (60--150 minutes) stimulated clearly the resting basal tone of the arteries. The possibility that the human plasma might have an enzyme(s) able to transform prostacyclin into a more stable material with human antiaggregatory platelet function and bovine coronary contracting capacity is discussed.
D015121:6-Ketoprostaglandin F1 alpha; D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D001792:Blood Platelets / Q000378:metabolism*; D001798:Blood Proteins / Q000494:pharmacology; D002417:Cattle; D003331:Coronary Vessels / Q000502:physiology*; D011464:Epoprostenol / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D011453:Prostaglandins / Q000378:metabolism*; D011460:Prostaglandins F / Q000494:pharmacology; D011465:Prostaglandins, Synthetic / Q000494:pharmacology
D000925:Anticoagulants; D001798:Blood Proteins; D011453:Prostaglandins; D011460:Prostaglandins F; D011465:Prostaglandins, Synthetic; D015121:6-Ketoprostaglandin F1 alpha; D000244:Adenosine Diphosphate; D011464:Epoprostenol