Reduction of non-specific radioactive counts in a whole blood microtiter plate assay of mitogenesis.
Non-specific radioactive counts retained on the glass fiber filters from a multiple automatic sample harvester were reduced markedly by 3 simple changes in the procedure for harvesting thymidine-labeled whole blood cultured from microtiter plates. These modifications increased the sensitivity, precision and accuracy of the lymphocyte transformation assay.
D001773:Blood Cells / Q000528:radiation effects*; D002478:Cells, Cultured; D006801:Humans; D007158:Immunologic Techniques; D008213:Lymphocyte Activation; D008938:Mitosis