The antigonadotropic activity of Lithospermum ruderale. II. The inhibition of LRF-induced gonadotropin release in vitro.
The antigonadotropic activity of lithosperm extracts was investigated at the pituitary level. When lithosperm extracts were added to primary pituitary cell cultures, LRF-stimulated LH release was inhibited. The cellular LH content was reduced but basal release was not significantly affected. These results suggest that lithosperm acts to directly inhibit the ability of LRF to stimulate LH release from the gonadotropes and the ability of the gonadotropes to produce LH.
D000818:Animals; D007987:Gonadotropin-Releasing Hormone / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D010902:Pituitary Gland / Q000187:drug effects / Q000502:physiology*; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000032:analysis; D051381:Rats
D010936:Plant Extracts; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone