Development of a protein A enzyme immunoassay for use in screening hybridomas.
A protein A enzyme immunoassay has been developed which is effective in the rapid screening of hybridomas. The detecting reagent is a stable alkaline phosphatase-protein A conjugate, prepared by a simple, inexpensive, single-step procedure. The assay requires small amounts of antigen to coat microtiter wells (less than 25 microgram/well) and can be evaluated visually or with a spectrophotometer. It compares favorably in sensitivity to a protein A-radioimmunoassay and enjoys considerably lower backgrounds than that popular screening procedure.
D000818:Animals; D002999:Clone Cells / Q000276:immunology; D006822:Hybrid Cells / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000032:analysis*; D011817:Rabbits; D011863:Radioimmunoassay; D013205:Staphylococcal Protein A / Q000276:immunology
D007136:Immunoglobulins; D013205:Staphylococcal Protein A