Galactose and galactose-1-phosphate spot test for galactosemia screening.
A simple spot test to measure galactose and galactose-1-phosphate in blood-impregnated filter paper was studied as a screening test for galactosemia in the newborn. A 3-mm disc punched from blood-impregnated filter paper card was fixed with acetone-methanol and incubated in a reaction mixture containing galactose dehydrogenase and alkaline phosphatase. This reaction mixture was then spotted on DEAE-cellulose paper, dried, and observed under a UV-lamp. The minimum amount of galactose detected by this procedure was 2 mg/dl. Estimation of galactose and galactose-1-phosphate with this procedure correlated well with estimation by bacterial assay.
D004926:Escherichia coli / Q000378:metabolism; D005453:Fluorescence; D005690:Galactose / Q000032:analysis*; D005693:Galactosemias / Q000175:diagnosis*; D005695:Galactosephosphates / Q000097:blood*; D006600:Hexosephosphates / Q000097:blood*; D006801:Humans; D009243:NAD / Q000032:analysis
D005695:Galactosephosphates; D006600:Hexosephosphates; D009243:NAD; C029973:galactose-1-phosphate; D005690:Galactose