Formation of hydroxypyruvaldehyde phosphate in human erythrocytes.
Human erythrocytes when incubated with [U-14C]glucose formed radioactively labeled hydroxypyruvaldehyde-P (CHOCOCH2OPO3(2-)). Using a "diagonal" chromatographic procedure on an anion exchange column for isolation and quantitative detection, up to 0.3 mumol of hydroxypyruvaldehyde-P/liter of packed cells was found. If glycolysis was blocked by inhibition of glyceraldehyde-3-P dehydrogenase with N-ethylmaleimide and iodoacetate, 0.7 mumol of hydroxypyruvaldehyde-P/liter of packed erythrocytes was detected. Hydroxypyruvaldehyde-P, found for the first time in eukaryotic cells, might be one of the long sought natural substrates of the glyoxalases.
D000447:Aldehydes / Q000097:blood*; D001786:Blood Glucose / Q000378:metabolism; D002250:Carbon Radioisotopes; D004912:Erythrocytes / Q000378:metabolism*; D006037:Glyoxal / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000097:blood*; D007700:Kinetics; D009943:Organophosphorus Compounds / Q000096:biosynthesis / Q000097:blood; D011865:Radioisotope Dilution Technique
D000447:Aldehydes; D001786:Blood Glucose; D002250:Carbon Radioisotopes; D009943:Organophosphorus Compounds; C008048:hydroxymethylglyoxal phosphate; D006037:Glyoxal