title: Stereological analysis of normal rabbit pancreatic islets.
abstract: Stereological methods were applied to obtain morphometric data related to pancreatic islets of Langerhans in the normal rabbit. By light microscopy, it was found that 1 mm3 of pancreatic parenchyma contained 47.7 islets, constituting 2.2% of its volume. Approximately 69% of the islets had diameters less than 80 micrometer; 31% were greater than 80 micrometer. The former group of islets, however, composed only 13% of the volume of the endocrine pancreas and the latter group, 87%. Using electron microscopy, a unit volume of islet tissue was observed to consist of 86% beta cells, 7.7% alpha cells, and 2.2% delta cells. The average beta-cell volume was 1260 micrometer 3 and its cytoplasm consisted of 52.6% matrix, 12.7% rough endoplasmic reticulum, 10.2% secretory granules, 7.8% mitochondria, and 3.3% Golgi apparatus. A typical beta cell contained 662 mitochondria, intermediate (10-nm) filaments whose length totalled 50 mm, and 9,200 secretory granules with a ratio of four mature granules to each immature or "pale" granule. Within alpha and beta cells, three parameters were used for the quantitation of organelles or their component parts: 1) volume; 2) surface; and 3) numerical densities. In the beta cell, the surface densities of rough endoplasmic reticulum and Golgi membranes exceeded, by two- or threefold, their counterparts in alpha cells. Similarly, the number of beta-cell mitochondria exceeded by 30% that of alpha cells; but beta-cell mitochondrial volume was twice that of the alpha cell, as were surface densities of both inner and outer mitochondrial membranes. Volume and surface densities of secretory granules within beta cells were half the values obtained for alpha cells. An alpha cell contained three times the number of granules present in a beta cell.
mesh_terms: D000818:Animals; D002452:Cell Count / Q000662:veterinary; D006652:Histological Techniques / Q000662:veterinary; D007515:Islets of Langerhans / Q000166:cytology / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D009940:Organoids / Q000648:ultrastructure; D011817:Rabbits / Q000033:anatomy & histology*