[Newer aspects of the electrophoretic mobility test after stimulation of human lymphocytes with PPD].
The Electrophoresis-Mobility-Test is a modified Macrophage-Electrophoretic-Mobility-Test by using sheep erythrocytes, tanned and stabilized by sulfosalicyl acid. The test is used to demonstrate specific sensibilised T-lymphocytes in cell culture after having them isolated from human venous blood and after stimulation with antigen. 34 tests were done by stimulation with antigen PPD, the lymphocytes having been taken from patients with positive Tuberculin-Tine-Test. All tests were bacteriologically and cytologically controlled and only 9 could be used finally as a base for statistical control by using x2- und t-tests and histogram. A special attention was payed to the aggregation of monocytes and their morphological changes in cell culture. Every single test is not statistically significant, but the results of the whole lot give an attest of the concept of the EM-Test, which can be reproduced with a probability of about 60%.
D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D008213:Lymphocyte Activation; D009000:Monocytes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014373:Tuberculin / Q000276:immunology*; D014374:Tuberculin Test; D014397:Tuberculosis, Pulmonary / Q000276:immunology
D014373:Tuberculin
Neuere Aspekte des Elektrophorese-Mobilitätstests nach Stimulation humaner Lymphozyten mit PPD.