A direct fluoroimmunoassay for conjugated chenodeoxycholic acid using antibody coupling to magnetisable particles.
A novel solid phase fluoroimmunoassay for conjugated chenodeoxycholic acid has been developed employing an antiserum coupled to magnetisable cellulose particles and a chenodeoxycholyl glycinefluorescein thiocarbamyl ethylene diamine conjugate as the label. The data obtained from serum samples from 25 patients correlated closely (r = 0.99) with those obtained by radioimmunoassay. The assay is rapid (30 minutes), simple, and ideal for routine clinical purposes.
D002482:Cellulose; D002635:Chenodeoxycholic Acid / Q000032:analysis*; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006801:Humans; D008280:Magnetics; D011863:Radioimmunoassay
D005452:Fluoresceins; D002635:Chenodeoxycholic Acid; C027101:fluorescein thiocarbamylethylenediamine; D002482:Cellulose