[Bf-immunofixation on cellogel membranes (author's transl)].
A method for the determination of Bf phenotypes of means of immunofixation after electrophoresis on cellogel membranes is reported. In comparison to immunofixation after agarose gel thin layer electrophoresis, this method shows an equal separation quality, but requires only about 1/5 of the monospecific anti-Bf serum, and results can be obtained after only 5 h. The gene frequencies calculated from our material (BfS 0.7994, BfF 0.1850, BfSO,7 0.0085, BfF1 0.0071) are in good accordance with investigations about other European populations.
D001604:Berlin; D011415:Complement Factor B / Q000302:isolation & purification*; D004792:Enzyme Precursors / Q000302:isolation & purification*; D005787:Gene Frequency; D006801:Humans; D007106:Immune Sera; D007158:Immunologic Techniques; D010641:Phenotype; D013997:Time Factors
D004792:Enzyme Precursors; D007106:Immune Sera; D011415:Complement Factor B
Bf-Immunfixation auf Cellogel-Folien.