Detection of Chlamydia trachomatis in rapidly produced McCoy cell monolayers.
The 24-hour delay between seeding coverslips with cells and inoculating samples for culture of chlamydiae was reduced to less than 1 hour by using coverslips which had been pre-treated with glutaraldehyde-activated gamma-aminopropyl-triethoxysilane. Treated coverslips were not toxic for McCoy cells and even one year after treatment monolayers formed rapidly on them. Furthermore, all of 13 Chlamydia trachomatis serotypes and one C. psittaci strain tested produced inclusions in such cell monolayers. In comparative tests, when there were large numbers of inclusions, more were always seen in conventionally produced monolayers than in monolayers on treated coverslips. However, when there were few inclusions, more were seen in the latter monolayers, a phenomenon observed with unpassaged chlamydiae in clinical specimens as well as in laboratory-passaged strains. The rapid method is, therefore, as sensitive for isolating chlamydiae as using conventionally produced monolayers.
D001431:Bacteriological Techniques; D002478:Cells, Cultured; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D013997:Time Factors