A simple rapid method for the preparation of en face endothelial (Häutchen) monolayers from rat and rabbit aortas.
This report describes a new method for preparing Häutchen monolayers for autoradiography with photographic emulsion contacting either the abluminal or luminal endothelial surface. Included is a means for monitoring completeness of the monolayer and retrieving cells which fail to adhere to it. Cell retrieval may be important in studies requiring autographic evaluation of endothelial cell populations. The procedure has been applied to endothelium containing newly reendothelialized aortic regions.
D000818:Animals; D001011:Aorta / Q000166:cytology*; D001345:Autoradiography / Q000379:methods*; D003584:Cytological Techniques; D004727:Endothelium / Q000166:cytology; D008297:Male; D011817:Rabbits; D051381:Rats