Synthesis of 3'-half molecule (nucleotides 36-76) of yeast alanine transfer ribonucleic acid.
We have successfully ligated the 12 nucleotide fragment (nucleotides 46-57) with the p19p nucleotide fragment (nucleotides 58-76) to form a 31p nucleotide fragment (nucleotides 46-76) which after 5'-phosphorylation was in turn joined to the 10 nucleotide fragment (nucleotides 36-45) to give the 3'-half molecule of yeast alanine tRNA.
D001483:Base Sequence; D007202:Indicators and Reagents; D008722:Methods; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D012346:RNA, Transfer, Amino Acyl / Q000138:chemical synthesis*; D012441:Saccharomyces cerevisiae
D007202:Indicators and Reagents; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D012346:RNA, Transfer, Amino Acyl