Receptor binding of biosynthetic human insulin on isolated pig hepatocytes.
Biosynthetic human insulin (BHI) and pancreatic human insulin were compared with respect to receptor binding in a heterologous assay system: displacement of pork A14-125I-monoiodoinsulin from receptors on pig hepatocytes. The concentrations of human insulin giving half-maximal displacement were identical for both preparations, i.e., 0.5 nM. Their relative potency was 1.01 +/- 0.14 (SD, N = 5), suggesting that biosynthetic and pancreatic human insulin exert the same biologic activity.
D000818:Animals; D001667:Binding, Competitive; D006801:Humans; D007328:Insulin / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000378:metabolism*; D007457:Iodine Radioisotopes; D007515:Islets of Langerhans; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D011972:Receptor, Insulin / Q000378:metabolism*; D013552:Swine
D007328:Insulin; D007457:Iodine Radioisotopes; C008147:insulin, iodo-; D011972:Receptor, Insulin