[Preparative isolation of polyribosomes from E. coli, and examination of their properties].
The paper describes a preparative method to produce polyribosomes from E. coli via their precipitation in a step-by-step sucrose gradient in the Ti-15 zonal rotor. Polyribosomes had the ability to translate mRNA in vitro. Polyribosomal proteins were isolated by the method of two-dimensional electrophoresis. The elongation factor EF-G was shown to occur in the polyribosomes.
D002458:Cell Fractionation / Q000379:methods; D002501:Centrifugation, Zonal / Q000379:methods; D004926:Escherichia coli / Q000378:metabolism*; D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors / Q000032:analysis; D011132:Polyribosomes / Q000378:metabolism / Q000648:ultrastructure*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012269:Ribosomal Proteins / Q000302:isolation & purification
D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors; D012333:RNA, Messenger; D012269:Ribosomal Proteins
Preparativnoe poluchenie poliribosom iz Escherichia coli i izuchenie ikh svoĭstv.