Ultrastructural alteration of rat cardiac myofibrils caused by a myosin-cleaving protease.
Incubation of rat cardiac myofibrils with a myosin-cleaving protease in the presence of EDTA at 25 degrees C caused removal of Z- and M-lines, accompanied by myofibril fragmentation. When rat cardiac muscle I-Z-I brushes were subjected to proteolysis, the protein. Ca2+ was not required for the ultrastructural alterations of myofibrils caused by the protease action.
D000818:Animals; D004587:Electrophoresis, Agar Gel; D008854:Microscopy, Electron; D009206:Myocardium / Q000648:ultrastructure*; D009210:Myofibrils / Q000187:drug effects* / Q000648:ultrastructure; D009218:Myosins / Q000037:antagonists & inhibitors; D010447:Peptide Hydrolases / Q000494:pharmacology*; D051381:Rats
D010447:Peptide Hydrolases; D009218:Myosins