Cryopreservation of human cadaver corneas regenerated at 31 degrees C in a modified tissue culture medium.
Human corneas were obtained 2-82 h post mortem, cultured for 20-28 h, in a modified tissue culture medium, frozen at a controlled rate, and thawed rapidly. The thawed corneas were subjected to 20-28 h of additional culture. Immediately after thawing, a mean endothelial cell damage of 11% was indicated by trypane blue staining. The mean endothelial cell loss during the subsequent culture was 34%. This cell loss was not related to post mortem time, to donor age, to cell loss during the primary culture, or to endothelial cell density.
D002102:Cadaver; D002452:Cell Count; D003080:Cold Temperature; D003315:Cornea / Q000166:cytology; D016039:Corneal Transplantation; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D004727:Endothelium / Q000166:cytology; D006801:Humans; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*; D014184:Transplantation, Homologous
D003470:Culture Media