Processing of alkaline phosphatase precursor to the mature enzyme by an Escherichia coli inner membrane preparation.
An inner membrane preparation co-translationally cleaved both the alkaline phosphatase and bacteriophage f1 coat protein precursors to the mature proteins. Post-translational outer membrane proteolysis of pre-alkaline phosphatase generated a protein smaller than the authentic monomer.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003090:Coliphages; D004792:Enzyme Precursors / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism* / Q000648:ultrastructure; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000378:metabolism
D001426:Bacterial Proteins; D004792:Enzyme Precursors; D011498:Protein Precursors; D000469:Alkaline Phosphatase