Evaluation of an enzyme immunoassay for serum gentamicin.
The EMIT (Enzyme Multiplied Immunoassay Technique) for serum gentamicin determination was evaluated by a standard procedure. The precision, accuracy, and specificity were assessed and proved satisfactory. Comparison with a bioassay was done, and results for patient samples showed a correlation coefficient of 0.95 between the two methods. Advantages of the enzyme immunoassay system were the provision of results within 15 min of receipt of blood in the laboratory, a requirement of minimal technical expertise, and an applicability to both large and small workloads. The EMIT proved during evaluation to be a practical alternative to current bioassays for the determination of serum gentamicin concentrations.
D002228:Carbenicillin / Q000097:blood; D004347:Drug Interactions; D004355:Drug Stability; D005839:Gentamicins / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques; D013982:Ticarcillin / Q000097:blood
D005839:Gentamicins; D013982:Ticarcillin; D002228:Carbenicillin