Surface domains of the guinea pig sperm defined with monoclonal antibodies.
Four monoclonal antibodies have been produced that recognize surface molecules of the guinea pig sperm. Each antibody binds to a unique, localized region of the sperm surface. The four antibody-binding patterns are restricted to the anterior head, of the sperm, the posterior head, the whole head and the posterior tail. Based on absorption experiments, the surface molecules detected by the antibodies are differentiation antigens. Three of the antibodies immunoprecipitate 125I-labeled protein antigens. The molecular weights of the polypeptides observed after SDS-PAGE are 52,000 (anterior head), 42,000 (whole head), and 60,000 (posterior head). The observed monoclonal antibody-binding patterns suggest that the guinea pig sperm surface is divided into a minimum of four domains.
D000818:Animals; D000906:Antibodies; D000954:Antigens, Surface / Q000032:analysis*; D002454:Cell Differentiation; D002999:Clone Cells; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006822:Hybrid Cells; D008297:Male; D008970:Molecular Weight; D013045:Species Specificity; D013077:Sperm Head / Q000276:immunology; D013082:Sperm Tail / Q000276:immunology; D013094:Spermatozoa / Q000276:immunology*
D000906:Antibodies; D000954:Antigens, Surface