X-ray microanalysis of the kidney.
The use of x-ray microanalysis for the localization of insoluble deposits in kidney tissue, the quantitation of micropuncture samples from tubular lumens, and the localization and quantitation of histochemical reaction products are becoming standard procedures in a variety of laboratories involved in renal research. The validity of these procedures has been established and exploitation of them should lead to new research insights. Even more exciting are the new analytical procedures for quantitative analysis of soluble substances in tissue sections. Shock frozen kidney can be analyzed in the frozen hydrated, frozen dried, or freeze substituted state. The promise of applying these technical achievements to renal research is a more precise understanding of the ion concentrations in lumens, intersititium, cells, and their organelles from various regions of the kidney in documented physiological and pathological states.
D000818:Animals; D004577:Electron Probe Microanalysis / Q000379:methods*; D004602:Elements; D005612:Freeze Drying / Q000379:methods; D005629:Frozen Sections; D006651:Histocytochemistry; D006801:Humans; D007668:Kidney / Q000032:analysis* / Q000648:ultrastructure; D007674:Kidney Diseases / Q000175:diagnosis*; D013048:Specimen Handling / Q000379:methods
D004602:Elements