A rapid immunoperoxidase assay for determination of IgG antibodies to measles virus.
A new indirect peroxidase antibody to membrane antigen (IPAMA) technique for the detection of IgG specific antibodies against measles virus is described. The technique utilizes as antigen measles-infected Vero cells dried on glass slides and stored at --70 degrees C. Sera of 509 healthy medical students and laboratory workers and 24 sera of measles, encephalitis, and subacute sclerosing panencephalitis (SSPE) patients were checked by IPAMA and the results have been compared with the results obtained by the hemagglutination-inhibition (HI) test. There is good agreement between the results of both techniques as to the presence or absence of antibody in 48 out of the 50 tested. The advantages of the techniques are discussed.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002460:Cell Line; D002522:Chlorocebus aethiops; D004660:Encephalitis / Q000097:blood; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D007668:Kidney; D008457:Measles / Q000097:blood; D008459:Measles virus / Q000276:immunology*; D013344:Subacute Sclerosing Panencephalitis / Q000097:blood
D000914:Antibodies, Viral; D007074:Immunoglobulin G