Benzene-induced myelotoxicity: application of flow cytofluorometry for the evaluation of early proliferative change in bone marrow.
A detailed description of flow cytofluorometric DNA cell cycle analysis is presented. A number of studies by the author and other investigators are reviewed in which a method is developed for the analysis of cell cycle phase in bone marrow of experimental animals. Bone marrow cell cycle analysis is a sensitive indicator of changes in bone marrow proliferative activity occurring early in chemically-induced myelotoxicity. Cell cycle analysis, used together with other hematologic methods, has revealed benzene-induced toxicity in proliferating bone marrow cells to be cycle specific, appearing to affect a population in late S phase which then accumulate in G2/M.
D000818:Animals; D001554:Benzene / Q000633:toxicity*; D001853:Bone Marrow / Q000473:pathology*; D001855:Bone Marrow Diseases / Q000139:chemically induced* / Q000188:drug therapy; D002455:Cell Division / Q000187:drug effects; D003584:Cytological Techniques; D004247:DNA / Q000096:biosynthesis; D005470:Fluorometry; D006801:Humans
D004247:DNA; D001554:Benzene