The lipid A moiety of lipopolysaccharide is specifically bound to B cell subpopulations of responder and nonresponder animals.
Employing autoradiography to detect [125I]-lipid A binding splenocytes and immunofluorescence to identify splenic B cells, it was observed that only a subpopulation of B cells bound lipid A. In contrast, non-B cells, including T cells and macrophage cells, exhibited either relatively little or no lipid A binding. LPS-responder and LPS-nonresponder substrains of mice exhibited essentially the same distribution of bound [125I]-lipid A among B cell subpopulations. Approximately 25% of the B splenocytes obtained from the LPS-responsive C3H/HeN substrain or from the LPS-nonresponsive C3H/HeJ, C57BL/10ScCR and C57BL/10ScN substrains bound [125I]-lipid A. On the other hand, 60% of the B splenocytes from both high- and low-responder mice derived from the CBA strain exhibited [125I]-lipid A binding. Taken in conjunction with the findings of other workers, these results are consistent with the possibility that the genetic defect in nonresponder mice is related to a lipid A triggering event rather than to lipid A binding.
D000818:Animals; D001345:Autoradiography; D001402:B-Lymphocytes / Q000145:classification*; D001665:Binding Sites; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005455:Fluorescent Antibody Technique; D007457:Iodine Radioisotopes; D008050:Lipid A / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell
D007457:Iodine Radioisotopes; D008050:Lipid A; D008070:Lipopolysaccharides; D011947:Receptors, Antigen, B-Cell