Antibodies against the subunits of E. coli RNA polymerase as probes for subunit-specific binding of DNA and other ligands.
Antibodies against the isolated subunits alpha, beta, and beta' of DNA-dependent RNA polymerase from E. coli have been prepared. They have been used to compare the extent of antibody-binding, as measured by complement fixation, to the isolated subunits and to the intact enzyme, in the absence and presence of ligands, such as inhibitors, nucleotides, nucleosides, oligo- and poly-nucleotides, and DNA of different composition. In many cases the results show a subunit-specific dependence of complement fixation upon the presence of a ligand and suggest a functional topography of the interaction between the subunits alpha, beta, and beta' of RNA polymerase and defined nucleotide sequences and small ligands.
D000907:Antibodies, Bacterial; D001665:Binding Sites; D003168:Complement Fixation Tests; D004247:DNA / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000276:immunology*; D004926:Escherichia coli / Q000201:enzymology*; D009705:Nucleosides / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism; D011487:Protein Conformation; D012293:Rifampin / Q000494:pharmacology
D000907:Antibodies, Bacterial; D009705:Nucleosides; D009711:Nucleotides; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin