Characterization of Mg2+-induced conformational change in the 50S ribosomal subunit by differential hydrogen exchange.
The technique of differential hydrogen exchange allows detection of a conformational change in the 50S subunit of Escherichia coli ribosome when the magnesium concentration is lowered in a range where ribosomal activity is fully preserved. This change is characterized by a seventy-fold acceleration of about thirty labile hydrogens in the case of a Mg2+ jump from 10 mM to 2 mM. The small number of hydrogens involved can explain the difficulty in detecting this change by other methods.
D004926:Escherichia coli / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology*; D008968:Molecular Conformation; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*
D006859:Hydrogen; D008274:Magnesium