[Chemical modification of phenylalanyl-tRNA synthetase and ribosomes of Escherichia coli with derivatives of tRNA-Phe carrying photoreactive groups on guanosine residues].
Photoreactive derivatives of tRNAPhe (E. coli) were synthesized by alkylation of the tRNAPhe with 4-(N-2-chloroethyl-N-methylamino)benzylamine and subsequent treatment with 1.4--dinitro-5-fluorophenylazide. The derivatives are active in binding with ribosomes and phenylalanyl-tRNA synthetase when the extent of modification is lower than 3 reactive groups per tRNAPhe molecule. Under irradiation the derivatives modify exclusively the beta-subunit of the phenylalanyl-tRNA synthetase.
D000588:Amines; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D001386:Azides; D001596:Benzylamines; D004139:Dinitrofluorobenzene / Q000031:analogs & derivatives; D004926:Escherichia coli / Q000378:metabolism*; D006151:Guanosine; D008722:Methods; D009150:Mustard Compounds; D009690:Nucleic Acid Conformation; D010649:Phenylalanine; D010652:Phenylalanine-tRNA Ligase / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D000588:Amines; D001386:Azides; D001596:Benzylamines; D009150:Mustard Compounds; D006151:Guanosine; C025881:4(N)-(2-chloroethyl-N-methylamino)benzylamine; D010649:Phenylalanine; C025880:2,4-dinitro-5-fluorophenylazide; D004139:Dinitrofluorobenzene; D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase
Modifikatsiia fenilalanil-tRNKrhe, nesushchimi reaktsionnosposobnye gruppy na ostatkakh guanozina.