[Formal genetic study of the enzymology of T4 phage recombination. I. Effect of the antimutational T4 DNA-polymerase on noncorrection-type recombination].
Effect of temperature sensitive antimutator L42 allele of gene 43 of T4 phage on the parameters of non-correction-type mechanism of genetic recombination have been studied under permissive conditions. By comparison of a point mutant with a deletion one, the critical frequency R (xi) corresponds to recombination between two markers separated by a distance equal to mean genetic length of the hybrid region was measured to be 4.15-10(-2). This value exceeds the frequency R (xi) determined with the parallel data in normal (43+) crosses by a factor of 1.6. The length of the hybrid region was shown not to change in the presence of L42 allele. The frequency of formation of the hybrid regions with recombinant flanks and of those with parental flanks increases in the presence of L42 by a factor of 1.3 and 1.8 respectively.
D000483:Alleles; D004259:DNA-Directed DNA Polymerase / Q000235:genetics* / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D009154:Mutation; D011995:Recombination, Genetic; D013604:T-Phages / Q000201:enzymology / Q000235:genetics*
D004259:DNA-Directed DNA Polymerase
Formal'no-geneticheskoe izuchenie énzimologii rekombinatsii u faga T4. Soobshchenie I. Vliianie antimutatornoĭ DNK-polimeraxy T4 na rekombinatsiiu nekorrektsionnogo tipa.