Immunohistochemistry of epithelial cell markers in normal and pathological colon mucosa. Comparison of results based on routine formalin- and cold ethanol-fixation methods.
The purpose of this study was to examine whether formal-dehyde-fixed tissue may afford reproducible and reliable immunhistochemical results when carcinoembryonic antigen (CEA), secretory component (SC), and epithelial IgA are evaluated semiquantitatively in normal and pathological colon specimens. Proximate tissue samples were processed by routine formalin fixation and by a cold-ethanol fixation method, respectively, and the immunofluorescence intensities obtained for the three antigens were scored. After formalin fixation SC and epithelial IgA were generally undetectable and also the staining for CEA was markedly reduced compared with that seen after ethanol fixation. Significant antigenic "unmasking" was obtained by enzyme treatment of the formalin-fixed tissue sections--resulting in enhanced staining for SC and epithelial IgA but not consistently so for CEA. With this modification scores from duplicate tissue samples processed by the two methods showed significant correlations for all the three epithelial markers; small amounts of CEA and epithelial IgA, and especially SC, nevertheless remained undetectable after formalin fixation. This result should be taken into account when epithelial markers are applied in studies of premalignant lesions of the colon where minor changes in the antigen pattern may be of diagnostic importance.
D002272:Carcinoembryonic Antigen / Q000032:analysis; D003093:Colitis, Ulcerative / Q000378:metabolism*; D003106:Colon / Q000032:analysis*; D003110:Colonic Neoplasms / Q000032:analysis*; D004847:Epithelial Cells; D005404:Fixatives / Q000592:standards; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007413:Intestinal Mucosa / Q000032:analysis; D012635:Secretory Component / Q000032:analysis
D002272:Carcinoembryonic Antigen; D005404:Fixatives; D007071:Immunoglobulin A, Secretory; D012635:Secretory Component