Analytical and biological analyses of test materials from the synthetic fuel technologies. III. Use of sephadex LH-20 gel chromatography technique for the bioassay of crude synthetic fuels.
To determine the health effects associated with newly emerging energy technologies, we have subjected a group of synthetic fuels to mutagenicity evaluation, using the Ames Salmonella assay. Coupling of chemical fractionation to the mutagenicity assays was necessary. Fractions obtained by use of Sephadex LH-20 gel chromatography on crude-coal-derived oils and shale oil were tested for mutagenicity with strain TA98 (with Aroclor S9 mix). Mutagenicity results obtained with synthetic fuels were compared with those from a mixture of natural petroleum crude oils. Merits of the Sephadex LH-20 separation technique and precautions in interpreting experimental results are discussed.
D002850:Chromatography, Gel; D003031:Coal / Q000032:analysis; D005579:Fossil Fuels / Q000032:analysis*; D005648:Fuel Oils / Q000032:analysis; D006838:Hydrocarbons / Q000032:analysis; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis*; D011083:Polycyclic Compounds / Q000032:analysis; D012486:Salmonella typhimurium / Q000235:genetics
D003031:Coal; D005579:Fossil Fuels; D005648:Fuel Oils; D006838:Hydrocarbons; D009153:Mutagens; D011083:Polycyclic Compounds