title: The significance of pH variations in human albumin perfusion media during continuous hypothermic renal perfusion.
abstract: The variation of pH in perfusate during renal machine perfusion has been regarded exclusively as an effect of kidney metabolism. During preservation of 21 canine kidneys for 48 hours in a Gambro PF-2D machine the pH was measured in the human albumin perfusion medium used, and in control samples stored a 4 degrees C. An increase in pH from 7.21 (+/- 0.03) to 7.66 (+/- 0.10) was found in the machine perfusate. In the stored perfusion medium an increase from 7.21 (+/- 0.03) to 7.43 (+/- 0.20) was measured. We conclude that the pH change in perfusate is not a reliable parameter for the functional state of the kidney unless the spontaneous pH variation in the perfusion medium is simultaneously recorded and a correction made.
mesh_terms: D000418:Albumins; D000818:Animals; D004285:Dogs; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016030:Kidney Transplantation; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion / Q000295:instrumentation / Q000379:methods*; D012034:Refrigeration; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*
chemical_list: D000418:Albumins