One micrometre paraffin sections: an aid to interpretation of immunoperoxidase staining of immunoglobulins.
A technique is described for obtaining 1 micron sections of tissue embedded in modified paraffin wax using long-edged glass knives. Serial sections can be cut and stained by the immunoperoxidase method to demonstrate multiple antigens in a single cell or to confirm that the immunoglobulin within a cell is monotypic. The improved cytological detail seen in thin paraffin sections permits the more precise localization of intracellular staining.
D006801:Humans; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000032:analysis*; D008867:Microtomy / Q000379:methods*; D014066:Palatine Tonsil / Q000276:immunology; D010232:Paraffin
D007136:Immunoglobulins; D010232:Paraffin