Study of bovine pulmonary response to Pasteurella haemolytica: specificity of immunoglobulins isolated from the bovine lung.
A procedure is described for the isolation and purification of the immunoglobulins (Ig)A and IgG from pulmonary lavage fluid from calves. These Ig were isolated from calves experimentally exposed to Pasteurella haemolytica via aerosolization and from nonexposed calves. The specificity of these fractions toward P haemolytica was examined, using an indirect fluorescent antibody test and agglutination reactions. Specific antibody activity was detected in the IgA and IgG fractions from calves exposed to P haemolytica and IgG fractions from nonexposed calves. None of the isolated Ig agglutinated P haemolytica.
D000372:Agglutination Tests; D000818:Animals; D002417:Cattle / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D007070:Immunoglobulin A / Q000032:analysis / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000032:analysis / Q000302:isolation & purification; D007136:Immunoglobulins / Q000032:analysis*; D008168:Lung / Q000276:immunology*; D010325:Pasteurella / Q000276:immunology*
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007136:Immunoglobulins