Ultrastructural and biochemical studies of glycogenosome formation in ascites hepatoma AH 13 cells.
Appreciable numbers of glycogenosomes were found in ascites hepatoma cells. They were lined by a single or sometimes double membranous structure exhibiting weakly positive acid phosphatase activity. Some were closely related to the Golgi apparatus, and a few showed wrapping of glycogen particles. The glycogen extracted from ascites hepatoma AH 13 cells was of two major types: a normal adult liver type, and a muscle type. Only the latter type gave the Cotton effect on measurement of the ORD and CD spectra. Small particulate, muscle type glycogen was observed in the glycogenosomes. Autophagy of normal adult liver type glycogen was never found. The engulfment of muscle type glycogen was accompanied by high acid and neutral alpha-glucosidase activity levels.
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D001201:Ascites / Q000473:pathology; D006003:Glycogen / Q000032:analysis*; D006056:Golgi Apparatus / Q000648:ultrastructure; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D000520:alpha-Glucosidases / Q000032:analysis
D006003:Glycogen; D000135:Acid Phosphatase; D000520:alpha-Glucosidases