Rabbit erythrocyte band 3: a receptor for staphylococcal alpha toxin.
Enzymes known to specifically cleave the band 3 component of the rabbit erythrocyte membrane were found to reduce both the hemolytic sensitivity to and the binding of the alpha toxin of Staphylococcus aureus. Lectins which bind to band 3 also inhibited the toxin. Lectins which do not bind to band 3 have no effect. Purified band 3, isolated by affinity chromatography on a concanavalin A column, was homogeneous by polyacrylamide gel electrophoresis, had a molecular weight of 100 000, and inhibited the hemolytic activity of alpha toxin. Antibodies to the toxin-toxoid receptor were serologically indistinguishable from antiband 3.
D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism* / Q000494:pharmacology; D001798:Blood Proteins / Q000276:immunology / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis*; D006461:Hemolysis; D007106:Immune Sera; D037102:Lectins / Q000494:pharmacology; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000494:pharmacology; D011817:Rabbits; D011955:Receptors, Drug / Q000032:analysis*; D013211:Staphylococcus aureus
D001427:Bacterial Toxins; D001798:Blood Proteins; D007106:Immune Sera; D037102:Lectins; D008565:Membrane Proteins; D011955:Receptors, Drug; D010447:Peptide Hydrolases