A rapid technique for detecting IgGFc and complement receptors on T and B cells in the neonatally thymectomized and normal adult rat.
Numerous studies have demonstrated the presence of IgGFc and C3 receptors on mammalian lymphoid cells. However, the further characterization of these cells as T, B, or null has been cumbersome and time consuming. A simple method is described for surface fluorescent labeling of (DA X L)F1 rat cell suspensions indirectly with either anti-rat IgM (sIg+) or monoclonal anti-rat thymocyte (sT+) antibody and then rosetting with EA or EAC. Staphylococcus protein A had no significant effect on the level of sIg+ or sT+ attained. From 58-78% of IgGFc+ cells were SIg+ with 14-18% sT+ in the spleen, lymph nodes, and peripheral blood. Almost 100% of C3+ cells were sIg+. Neonatally thymectomized adults exhibited a 254-413% decrease in sT+ cells but a 154-329% increase in sIg+ and a 177-330% increase in null cells. They also exhibited a 140-400% increase in IgGFc+ and C3+ cells in all lymphoid compartments. Fifty-two to 65% of IgGFc+ cells and again almost 100% of C3+ were sIG+. The methods and results are discussed as a basis for studies of B and T lymphocyte maturation and function within various rat lymphoid compartments.
D000818:Animals; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology*; D002454:Cell Differentiation; D003176:Complement C3 / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D007141:Immunoglobulin Fc Fragments / Q000276:immunology*; D051381:Rats; D011971:Receptors, Immunologic / Q000032:analysis*; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D013934:Thymectomy; D013950:Thymus Gland / Q000166:cytology
D003176:Complement C3; D007141:Immunoglobulin Fc Fragments; D011971:Receptors, Immunologic