[Immunostimulant properties of an extract isolated and partially purified from Aloe vahombe].
When the mice are given a hypodermic infection of unrefined Vahombe extract, the Aloe called Vahombe is a liliaceous plant growing in the South of Madagascar, they are protected against the infection caused by the Klebsiella, a pneumonia vector to man, giving rise to an experimental septicaemia in the mouse. Neither bactericide nor bacteriostatic activity has been detected yet about Aloe extract. The anti-infectious activity is proportional to the dose of extract injected, the protecting power is the greatest when the mice have been treated with Aloe, two or three days previously to the infection due to Klebsiella pneumoniae. We have determined the LD50 (Lethal dose 50) for the check batches (non-treated mice) and for the batches of protected mice. We were able to show that the previous injection developed the resistance to infection, multiplied from thirtyfold to a hundrefold. We have tackled the purification of the substance--made soluble after lyophilisation of the crude extract--by means of filtration with Sephadex G50. It would be the first time, for all we know, that a substance endowed with organism. At present we are proceeding with the purification of the active principle and contemplating trying the protective power upon virus infections as well as upon cancerous or parasitic ones.
D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000504:Aloe / Q000032:analysis*; D000818:Animals; D007710:Klebsiella Infections / Q000517:prevention & control; D007711:Klebsiella pneumoniae; D007928:Lethal Dose 50; D051379:Mice; D010936:Plant Extracts / Q000627:therapeutic use*; D010946:Plants, Medicinal / Q000032:analysis*
D000276:Adjuvants, Immunologic; D010936:Plant Extracts
Mise en évidence et étude des propriétés immunostimulantes d'un extrait isole et partiellement purifié à partir d'Aloe vahombe.