Histamine-binding suppressor T cells in rabbit peripheral blood.
Peripheral blood leukocytes (PBL) from primed rabbits were able to suppress the in vitro anti-sheep red blood cell (SRBC) plaque-forming cell (PFC) response of autologous spleen cells. A population containing the suppressor cells could be isolated from PBL by cell fractionation on columns of insolubilized histamine. In contrast to spleen cells, PBL generatd a weak secondary anti-SRBC response in vitro. A strong response was obtained with PBL freed from histamine-binding (H+) cells. The addition of these H+ cells to cultures of H-PBL caused strong suppression. The H+ suppressor cell was further characterized as a radioresistant T cell. Low-dose irradiation of H- cells resulted in a supplementary enhanced PFC response suggesting that PBL also contain a radiosensitive regulator cell which is not histamine binding.
D000818:Animals; D000917:Antibody Formation; D005591:Chemical Fractionation; D004307:Dose-Response Relationship, Radiation; D005455:Fluorescent Antibody Technique; D006462:Hemolytic Plaque Technique; D011817:Rabbits; D011968:Receptors, Histamine; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D011968:Receptors, Histamine