title: Effect of tumor promoters on ultraviolet light-induced mutation and mitotic recombination in Saccharomyces cerevisiae.
abstract: Recently, it has been suggested that mitotic recombination is involved in tumor promotion. On this basis, one might expect tumor promoters to be recombinagenic. D7 is a diploid strain of yeast in which both mutation and mitotic recombination can be measured. We have used this strain to assay the known tumor promoters, iodoacetate, anthralin, and 12-O-tetradecanoylphorbol-13-acetate, and the cocarcinogen, catechol, for mutagenicity, recombinagenicity, and the ability to enhance ultraviolet light (UV)-induced genetic events. In the absence of preirradiation with UV, iodoacetate was found to be recombinagenic whereas catechol was mutagenic; however, in both cases, the effects were small. Iodoacetate, anthralin, and catechol potentiated UV-induced mitotic crossing-over, aberrant colony formation, and mutation, while catechol also increased UV-induced gene conversion. We were unable to detect any mutagenic or recombinagenic effect of 12-O-tetradecanoyl-phorbol-13-acetate in either whole cells or spheroplasts. Our results do not indicate any consistent correlation between tumor-promoting activity and the ability of an agent to induce mitotic recombination in yeast. However, the ability to potentiate UV-induced mutation and mitotic recombination may reflect the cocarcinogenic activity of certain promoters.
mesh_terms: D000873:Anthracenes / Q000494:pharmacology*; D000875:Anthralin / Q000494:pharmacology*; D002273:Carcinogens; D002396:Catechols / Q000494:pharmacology*; D003043:Cocarcinogenesis; D003434:Crossing Over, Genetic; D005785:Gene Conversion; D007461:Iodoacetates / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects; D009153:Mutagens; D009154:Mutation / Q000187:drug effects*; D010703:Phorbol Esters / Q000494:pharmacology; D011838:Radiation-Sensitizing Agents; D011995:Recombination, Genetic / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D000873:Anthracenes; D002273:Carcinogens; D002396:Catechols; D007461:Iodoacetates; D009153:Mutagens; D010703:Phorbol Esters; D011838:Radiation-Sensitizing Agents; D000875:Anthralin