Measurement of drug-metabolizing systems in Salmonella typhimurium strains G46, TA15135, TA100, TA1538 and TA98.
Salmonella typhimurium strains which are commonly used in the Ames test for screening potential carcinogens were examined for a number of drug-metabolizing systems. Neither cytochrome P-450 itself nor two activities catalyzed by the cytochrome P-450 system in mammalian cells, i.e., benzpyrene monooxygenase and ethoxycoumarin O-deethylation, could be detected. Nor do these bacterial strains demonstrate any ability to detoxify epoxides by hydrating them or to conjugate p-nitrophenol with glucuronic acid. On the other hand, S. tryphimurium strains G46, TA1535, TA100, TA1538 and TA98 contain considerable amounts of acid-soluble thiols, approx. 5--10% of which is glutathione. These bacteria can also enzymatically conjugate glutathione with 1-chloro-2,4-dinitrobenzene (CDNB) and can reduce oxidized glutathione using NADPH as cofactor. Thus, enzymatic and non-enzymatic reaction of immediate carcinogens with thiol groups in s. typhimurium may have a significant effect on the outcome of the Ames test in certain cases.
D001426:Bacterial Proteins / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000254:growth & development / Q000378:metabolism*; D013045:Species Specificity; D013438:Sulfhydryl Compounds / Q000378:metabolism
D001426:Bacterial Proteins; D004364:Pharmaceutical Preparations; D013438:Sulfhydryl Compounds; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione