A simple, fast and inexpensive kinetic method to differentiate between total and viable bacteria using Coulter counter technique.
A new kinetic approach is described using Coulter Counter technique to differentiate between viable and total counts in bacterial cultures (E. coli) inhibited by chemotherapeutics (trimethoprim, tetracycline, and a nitrofurane derivative, 2,4-diamino-6-(5-nitrofuryl-2)-5-ethylpyrimidine (HN 32). The results are compared with data obtained by plate count technique.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques / Q000295:instrumentation*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D007700:Kinetics; D009581:Nitrofurans / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology; D014295:Trimethoprim / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D009581:Nitrofurans; D011743:Pyrimidines; C024574:2,4-diamino-6-(5-nitrofuryl-2)-5-ethylpyrimidine; D014295:Trimethoprim; D013752:Tetracycline