Enzyme-immunoassay for determination of progesterone in milk from cows.
An enzyme-immunoassay using horseradish peroxidase as label is described. The assay has a sensitivity of 0.1-20 pmol, i.e. 0.03-0.63 ng and intra- and inter-assay coefficients of variation of less than 10% when used for the determination of progesterone in samples of milk from cows. Comparison with a radio-immunoassay based on tritiated tracer showed excellent correlation (r = 0.98) for milk samples from cows during the oestrous cycle and early pregnancy.
D000818:Animals; D002417:Cattle / Q000378:metabolism*; D003429:Cross Reactions; D004971:Estrus; D005260:Female; D007124:Immunoenzyme Techniques; D008892:Milk / Q000032:analysis*; D011247:Pregnancy; D011374:Progesterone / Q000032:analysis*; D011863:Radioimmunoassay
D011374:Progesterone