title: Immunologic properties of biosynthetic human insulin in vitro.
abstract: Some immunologic properties of biosynthetic human insulin (BHI) were examined in vitro. An identical behavior was found for BHI and pancreatic human insulin as standard preparation and for A14-mono-labeled BHI and pork insulin as tracer in the insulin radioimmunoassay. BHI proved to be free of human proinsulin and C-peptide. Insulin antibodies in serum of two diabetic patients showed a preferential binding of 125I-bovine insulin. However, the antibody titers were almost identical for A14-mono-labeled BHI and pork insulin. These studies did not reveal any characteristic immunologic properties of BHI compared with highly purified pancreatic human and pork insulin.
mesh_terms: D000818:Animals; D002417:Cattle; D003429:Cross Reactions; D006801:Humans; D007328:Insulin / Q000096:biosynthesis / Q000276:immunology*; D007330:Insulin Antibodies; D007515:Islets of Langerhans / Q000032:analysis; D011863:Radioimmunoassay; D013045:Species Specificity; D013552:Swine
chemical_list: D007328:Insulin; D007330:Insulin Antibodies