A procedure for the rapid preparation of 14C-aminoacyl-transfer RNA and its use in the assay of class I ribonucleases and ribonuclease inhibitor.
The presence of ribonuclease inhibitor and/or the activity of class I ribonucleases can conveniently be measured, at all stages of purification, by a highly sensitive assay based on the loss of radioactivity during the concomitant hydrolysis of tRNA and small amounts of 14C-labeled aminoacyl-tRNA. The rapid, economical assay, which is readily adaptable to homologous tRNA substrates, eliminates the necessity of filtration, centrifugation and ultraviolet spectroscopy measurements required by most other assays and is particularly suitable for multiple samples and kinetic measurements.
D000818:Animals; D002250:Carbon Radioisotopes; D007700:Kinetics; D008099:Liver; D010179:Pancreas / Q000201:enzymology; D012346:RNA, Transfer, Amino Acyl / Q000138:chemical synthesis*; D011865:Radioisotope Dilution Technique; D012260:Ribonucleases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013552:Swine; D013961:Thyroid Gland
D002250:Carbon Radioisotopes; D012346:RNA, Transfer, Amino Acyl; D012260:Ribonucleases