The structure of the capsular polysaccharide (K5 antigen) of urinary-tract-infective Escherichia coli 010:K5:H4. A polymer similar to desulfo-heparin.
The capsular polysaccharide was isolated from Escherichia coli 010:K5:H4; it could not be obtained from a uncapsulated (K5-) mutant. It contains N-acetylglucosamine and glucuronic acid in a molar ratio of 1:1. Acid hydrolysis of the acidic polysaccharide as well as Smith degradation and degradation by deamination of the carboxyl-reduced polysaccharide suggested that the polysaccharide is composed of a disaccharide repeating unit. The data obtained by methylation analysis and nuclear magnetic resonance spectroscopy indicated that the repeating sequence of the capsular polysaccharide is the 4-beta-glucuronyl-1,4-alpha-N-acetylglucosaminyl unit. This structure is similar to that of desulfo-heparin.
D000107:Acetylation; D000941:Antigens / Q000302:isolation & purification*; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D004926:Escherichia coli / Q000276:immunology*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D014552:Urinary Tract Infections / Q000382:microbiology*
D000941:Antigens; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D002241:Carbohydrates; C030659:K antigens