Triethyllead treatment of cultured brain cells. Effect on accumulation of radioactive precursors in galactolipids.
Cultured cells from chick embryo brains were studied for their sensitivity to triethyllead. Triethyllead chloride (3.16 microM) was added to the nutrient medium and incubated for 48 hr with the cells. Morphological changes in light microscope and radioactive labeling of galactolipids were assayed. Triethyllead treatment reduced the number of neuronal cells with processes. Morphological changes were not observed in glial cells. The [35S]sulfate labeling of sulfatides was reduced to 50%. The [3H]serine labeling of cerebrosides with alpha-hydroxy fatty acids was not influenced, while the [3H]serine labeling of cerebrosides with nonhydroxy fatty acids was inhibited 40% in one- and two- but not in three-week-old cultures. The results indicate that the nerve cell response to triethyllead in cultures is selective, since the neurons are more sensitive than the glia cells and the labeling of sulfatides is more sensitive than that of cerebrosides.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D002855:Chromatography, Thin Layer; D038983:Galactolipids; D006017:Glycolipids / Q000096:biosynthesis*; D007854:Lead / Q000494:pharmacology*; D009942:Organometallic Compounds / Q000494:pharmacology*; D011865:Radioisotope Dilution Technique; D013433:Sulfoglycosphingolipids / Q000096:biosynthesis; D013462:Sulfur Radioisotopes; D014316:Tritium
D038983:Galactolipids; D006017:Glycolipids; D009942:Organometallic Compounds; D013433:Sulfoglycosphingolipids; D013462:Sulfur Radioisotopes; D014316:Tritium; D007854:Lead; C010360:triethyllead