Detection of Yersinia enterocolitica heat-stable enterotoxin by suckling mouse bioassay.
A comparison of heat-stable enterotoxins from Escherichia coli and Yersinia enterocolitica by the suckling mouse bioassay showed that whereas E. coli heat-stable enterotoxin evoked a maximum ratio of gut weight to remaining body weight after a 4-h incubation period, the maximum ratio for Y. enterocolitica was achieved at 2 h, decreasing thereafter to values regarded as negative (less than 0.083). This action of Y. enterocolitica heat-stable enterotoxin may give false-negative results for the standard suckling mouse bioassay.
D000818:Animals; D000833:Animals, Suckling; D001681:Biological Assay / Q000379:methods*; D003967:Diarrhea / Q000382:microbiology; D004768:Enterotoxins / Q000032:analysis* / Q000494:pharmacology; D004926:Escherichia coli; D006358:Hot Temperature; D006801:Humans; D007223:Infant; D007422:Intestines / Q000187:drug effects; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D013045:Species Specificity; D015007:Yersinia
D004768:Enterotoxins