title: Changes in nucleus and surface of Lepidoptera cells cultivated in vitro after infection with nuclear polyhedrosis viruses (Autographa californica).
abstract: Changes in cell nuclei after infection of Lepidoptera cells, demonstrated by means of the fluorochrome "33 258H", permit the observation of virus DNA synthesis in a light microscope. Parallel occurring changes in cell surfaces, analysed in a scanning electron microscope, also permit an exact differentiation between infected and non-infected cells.
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D002467:Cell Nucleus / Q000378:metabolism / Q000382:microbiology* / Q000648:ultrastructure; D002472:Cell Transformation, Viral; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D007304:Insect Viruses / Q000378:metabolism*; D007814:Larva; D007915:Lepidoptera / Q000378:metabolism / Q000382:microbiology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D014779:Virus Replication
chemical_list: D004279:DNA, Viral