title: Visualization of cell-substratum adhesion plaques by antibody exclusion.
abstract: We report on an antibody exclusion method for detecting cell adhesion plaques. Substrata coated by plasma fibronectin )pFN) appeared uniformly fluorescent when analyzed by indirect immunofluorescence using a specific anti-pFN antibody. When cells that had spread on the substrata were permeabilized and analyzed by indirect immunofluorescence, areas of non-fluorescence were apparent beneath the cells and in focal streaks along the cell margins. The nonfluorescent focal streaks were coincident with adhesion plaques visualized by interference reflection microscopy.
mesh_terms: D002448:Cell Adhesion; D002462:Cell Membrane / Q000648:ultrastructure*; D002478:Cells, Cultured; D005347:Fibroblasts; D005353:Fibronectins / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008857:Microscopy, Interference
chemical_list: D005353:Fibronectins