The generation of haemolytic plaque-forming cells from human tonsils.
At low cell numbers (2 X 10(6) cells/culture) good numbers of plaque-forming cells (PFC) were obtained in response to stimulation by pokeweed mitogen (PWM). Maximum PFC were obtained with the Marbrook chamber using 2 X 10(6) cells/culture, stimulating cells with a 1:50 dilution of PWM and harvesting after 6--7 days. 20 different experiments using standard conditions gave a geometric mean of 646 PFC/10(6) initial cells. All PFC detected were IgM.
D000293:Adolescent; D000328:Adult; D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells; D002648:Child; D002675:Child, Preschool; D004912:Erythrocytes / Q000276:immunology; D006462:Hemolytic Plaque Technique; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D014066:Palatine Tonsil / Q000166:cytology / Q000276:immunology*; D011043:Pokeweed Mitogens / Q000494:pharmacology; D012756:Sheep
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011043:Pokeweed Mitogens