Plasmid transfer within and between serologically distinct strains of Rhizobium japonicum, using antibiotic resistance mutants and auxotrophs.
Methionine-requiring and pantothenic acid-requiring auxotrophs of Rhizobium japanicum USDA 31, as well as highly antibiotic-resistant mutants of R. japonicum strains USDA 31, USDA 110, USDA 138, and Webster 48, were isolated. These mutants were used to transfer the P-1 group plasmids R68.45 and RP4 within and between strains USDA 31, USDA 110, and Webster 48. Attempts to demonstrate transfer of either plasmid to strain USDA 138 were unsuccessful.
D000667:Ampicillin / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics; D007612:Kanamycin / Q000494:pharmacology; D010957:Plasmids; D011815:R Factors; D012231:Rhizobium / Q000235:genetics*; D013307:Streptomycin / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology; D014161:Transduction, Genetic; D014169:Transformation, Bacterial
D007612:Kanamycin; D000667:Ampicillin; D013752:Tetracycline; D013307:Streptomycin