The characterisation of a 5-S 'monosome' fraction from chromatin of Physarum polycephalum.
A so-called '5-S mononucleosome' fraction was isolated from nuclei of Physarum polycephalum by digestion with micrococcal nuclease and subsequent fractionation by gel filtration. This fraction had electrophoretic and sedimentation properties which were similar to DNA of approximately 140 base pairs in length. It is shown that lysis of the nuclei activates a protease which is resistant to phenyl-methyl sulphonyl fluoride, o-phenanthroline and parachloromercuri benzene sulphonate and which subsequently degrades the nucleosomes.
D002843:Chromatin / Q000032:analysis*; D002850:Chromatography, Gel; D004789:Enzyme Activation; D008836:Micrococcal Nuclease / Q000378:metabolism; D009707:Nucleosomes / Q000302:isolation & purification*; D010447:Peptide Hydrolases / Q000378:metabolism; D010804:Physarum / Q000235:genetics
D002843:Chromatin; D009707:Nucleosomes; D008836:Micrococcal Nuclease; D010447:Peptide Hydrolases