Effects of amiloride on insulin release, serum glucose and insulin, and glucose tolerance of mice.
Release of insulin was increased when isolated mouse islets were exposed to 1 or 2 mM amiloride, both in the presence of low (3 mM) and high (20 mM) glucose concentration. Fed mice injected with amiloride developed a hypoglycemia at 1, 2 and 4 h, and a hyperglycemia at 36 h, whereas no significant blood glucose alterations were observed in starved mice treated with amiloride. The serum insulin concentration was significantly increased 1 and 36 h following amiloride injection. Amiloride is believed to affect the islet beta-cells directly or indirectly, leading to ionic alterations, and stimulated insulin release.
D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D005260:Female; D005951:Glucose Tolerance Test; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011719:Pyrazines / Q000494:pharmacology*; D012636:Secretory Rate / Q000187:drug effects; D013997:Time Factors
D001786:Blood Glucose; D007328:Insulin; D011719:Pyrazines; D000584:Amiloride