Early dominance of irradiated host cells in the responder profiles of thymocytes from P leads to F1 radiation chimeras.
The number of cells in the thymus of [k leads to (b X k)F1] radiation (100 rad) chimeras increases approximately 10-fold between 7 and 14 days after reconstitution with bone marrow. At least 50% of the cells in thymus on day 14 are of host origin and respond to virus presented in the context of both H-2k and H-2b when primed in irradiated, virus-infected (b X k)F1 recipients. Strong CTL responses can be generated from thymocytes of donor origin on day 21. All evidence of a significant host thymocyte component has disappeared by day 28. The responsiveness of 14-day thymocytes is not abrogated by pretreatment of the mice used to make the chimeras with anti-thymocyte serum or by using doses of irradiation as high as 1200 rads to eliminate host components.
D000818:Animals; D000954:Antigens, Surface; D000961:Antilymphocyte Serum / Q000494:pharmacology; D016026:Bone Marrow Transplantation; D003165:Complement System Proteins; D003433:Crosses, Genetic; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006168:Guinea Pigs; D006410:Hematopoiesis; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011828:Radiation Chimera; D013601:T-Lymphocytes / Q000528:radiation effects*; D013997:Time Factors
D000954:Antigens, Surface; D000961:Antilymphocyte Serum; D003165:Complement System Proteins