Effects of L-cysteine and O-acetyl-L-serine in the synthesis and mutagenicity of azide metabolite.
The ability of L-cysteine to inhibit azide-metabolite synthesis and mutagenicity is investigated in Salmonella typhimurium TA1530 and cys E6 strains. L-cysteine specifically inhibits the synthesis of the mutagenic azide metabolite as other compounds containing SH group did not affect the production of this metabolite. Azide mutagenicity is completely inhibited by L-cysteine at a concentration (5 mumoles/plate) where the metabolite mutagenicity was not affected. O-Acetyl-L-serine can reverse the L-cysteine mediated inhibition of the metabolite synthesis and thus mutagenicity in the same strains. These results suggest that O-acetyl-L-serine may be required to synthesize the azide metabolite or its precursor.
D001386:Azides / Q000378:metabolism* / Q000494:pharmacology; D001711:Biotransformation / Q000187:drug effects*; D003545:Cysteine / Q000494:pharmacology*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000235:genetics
D001386:Azides; D009153:Mutagens; D003545:Cysteine