Effect of tumor promoters on ultraviolet light-induced mutation and mitotic recombination in Saccharomyces cerevisiae.
Recently, it has been suggested that mitotic recombination is involved in tumor promotion. On this basis, one might expect tumor promoters to be recombinagenic. D7 is a diploid strain of yeast in which both mutation and mitotic recombination can be measured. We have used this strain to assay the known tumor promoters, iodoacetate, anthralin, and 12-O-tetradecanoylphorbol-13-acetate, and the cocarcinogen, catechol, for mutagenicity, recombinagenicity, and the ability to enhance ultraviolet light (UV)-induced genetic events. In the absence of preirradiation with UV, iodoacetate was found to be recombinagenic whereas catechol was mutagenic; however, in both cases, the effects were small. Iodoacetate, anthralin, and catechol potentiated UV-induced mitotic crossing-over, aberrant colony formation, and mutation, while catechol also increased UV-induced gene conversion. We were unable to detect any mutagenic or recombinagenic effect of 12-O-tetradecanoyl-phorbol-13-acetate in either whole cells or spheroplasts. Our results do not indicate any consistent correlation between tumor-promoting activity and the ability of an agent to induce mitotic recombination in yeast. However, the ability to potentiate UV-induced mutation and mitotic recombination may reflect the cocarcinogenic activity of certain promoters.
D000873:Anthracenes / Q000494:pharmacology*; D000875:Anthralin / Q000494:pharmacology*; D002273:Carcinogens; D002396:Catechols / Q000494:pharmacology*; D003043:Cocarcinogenesis; D003434:Crossing Over, Genetic; D005785:Gene Conversion; D007461:Iodoacetates / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects; D009153:Mutagens; D009154:Mutation / Q000187:drug effects*; D010703:Phorbol Esters / Q000494:pharmacology; D011838:Radiation-Sensitizing Agents; D011995:Recombination, Genetic / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects; D014466:Ultraviolet Rays
D000873:Anthracenes; D002273:Carcinogens; D002396:Catechols; D007461:Iodoacetates; D009153:Mutagens; D010703:Phorbol Esters; D011838:Radiation-Sensitizing Agents; D000875:Anthralin