Bacterial contamination of corneal donor tissue.
We studied bacterial contamination of corneal donor material following processing by two techniques prior to immersion in McCarey-Kaufman medium. If the whole eye was immersed in Neosporin, 24.5% of cultures were positive. If eyes were only rinsed with Neosporin, 47.5% of cultures were positive. No eyes receiving this tissue developed infections. Cultures were much more likely to be positive if the donors had been on a respirator prior to death.
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D001419:Bacteria / Q000187:drug effects / Q000302:isolation & purification*; D002648:Child; D003315:Cornea / Q000382:microbiology*; D016039:Corneal Transplantation; D006801:Humans; D008875:Middle Aged; D009355:Neomycin / Q000494:pharmacology; D009926:Organ Preservation / Q000379:methods*; D012996:Solutions; D014021:Tissue Preservation / Q000379:methods*; D014184:Transplantation, Homologous
D012996:Solutions; D009355:Neomycin