Analysis of the composition of mixed lipid phases by the moments of 2H NMR spectra.
The use of (2)H NMR spectral moments to determine the composition of biphasic lipid mixtures is outlined. The analysis has been applied to phosphatidylethanolamine-cholesterol (1:1), potassium palmitate, 30% (wt/wt) water and phosphatidylcholine-cholesterol (4:1) systems, as well as to membrances of Escherichia coli during phase transitions. The advantages and disadvantages of the use of spectral moments to determine fractions of coexistent phases are discussed.
D002462:Cell Membrane / Q000378:metabolism; D002784:Cholesterol; D004926:Escherichia coli / Q000378:metabolism; D005782:Gels; D008081:Liposomes; D009682:Magnetic Resonance Spectroscopy; D008560:Membrane Fluidity; D010168:Palmitates; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines
D005782:Gels; D008081:Liposomes; D010168:Palmitates; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D002784:Cholesterol