Growth of Mycobacterium lepraemurium in cell-free liquid medium containing soluble starch: evaluation by generation time and morphological observation.
A stimulatory effect of soluble starch on the growth of M. lepraemurium in vitro cell-free culture system has been noted. In this medium bacteria elongated gradually without manifesting any significant peak in elongation as has been noted in NC-5 medium. The maximum average elongation of bacteria was 2.84 mu on the 24th day in NCS-5 medium. In contrast, in NC-5 bacteria elongated maximum on the 6th day (2.32 mu). All the stages of bacterial cell division were noted. Hypothesis has been suggested for continuous multiplication of M. lepraemurium in NCS-5 medium.
D002455:Cell Division; D003470:Culture Media; D008854:Microscopy, Electron; D009167:Mycobacterium lepraemurium / Q000254:growth & development* / Q000648:ultrastructure; D013213:Starch; D013997:Time Factors
D003470:Culture Media; D013213:Starch