Endothelial-monocyte antigens.
Monocyte cytotoxicity was found to be very dependent on selection of rabbit complement. Eluates obtained from kidney allografts were found to contain a variety of antibodies against monocytes, B cells, and T cells. Specific monocyte reactions were not blocked by an antiserum against human DR antigens made in chickens. A turkey serum against beta 2-microglobulin did abolish monocyte-specific reactions. Blocking with chicken anti-DR was used to assess antibodies in 56 alloantisera. Among the reactions observed, 52% were blocked by the anti-DR serum; 25% appeared to be monocyte-specific.
D000818:Animals; D000961:Antilymphocyte Serum / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D004727:Endothelium / Q000166:cytology / Q000276:immunology; D006084:Graft Rejection; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007519:Isoantigens / Q000032:analysis; D016030:Kidney Transplantation; D009000:Monocytes / Q000276:immunology*; D011817:Rabbits; D001613:beta 2-Microglobulin / Q000276:immunology
D000961:Antilymphocyte Serum; D000949:Histocompatibility Antigens Class II; D007519:Isoantigens; D001613:beta 2-Microglobulin; D003165:Complement System Proteins