Purification of hemopoietic progenitor cells from human marrow using a fucose-binding lectin and cell sorting.
Human peripheral blood granulocytes, but not lymphocytes, erythrocytes, or monocytes, bound the fucose-binding lectin from Lotus tetragonolobus (FBP), and this binding was competitively inhibited by the sugar alpha-L-fucose. The fluorescence-activated cell sorter was used to study the appearance of this receptor on human marrow cells during granulocyte differentiation and to prepare fractions enriched for granulocyte-macrophage progenitor cells (granulocyte-macrophage colony-forming cells--GM-CFC). Cell binding of fluoresceinated FBP increased for bone marrow cells in the sequence--lymphocytes, blast cells, promyelocytes and myelocytes, monocytes, and polymorphonuclear cells. Selection of cells with appropriate low-angle or high-angle light scatter characteristics achieved a 10-fold or 2-3-fold enrichment of progenitor cells, respectively. By selecting cells with intermediate fluorescence intensity, a further 2-3-fold enrichment for GM-CFC was obtained. Cell sorting using the optimal selection of these three parameters produced up to 36-fold enrichment of the progenitor cells from human bone marrow. The most enriched fraction was composed of 23% progenitor cells (colony- and cluster-forming cells) with a yield of 36%. In populations most highly enriched by GM-CFC, immature cells (blast cells, promyelocytes, and myelocytes) made up 95% of the cells present.
D001665:Binding Sites; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002469:Cell Separation; D003114:Colony-Forming Units Assay; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005643:Fucose / Q000378:metabolism*; D006412:Hematopoietic Stem Cells; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D008027:Light; D012542:Scattering, Radiation
D005452:Fluoresceins; D037102:Lectins; D005643:Fucose