title: Transfer of experimental autoimmune insulitis by spleen cells in mice.
abstract: We have tested whether experimental insulitis induced by multiple subdiabetogenic injections of streptozotocin can be transferred by lymphocytes to normal recipients. C57BL/6J mice were treated on 5 consecutive days with 40 mg streptozotocin/kg body weight. 5 X 10(7) nucleated spleen cells from 20 animals which had developed hyperglycaemia with concomitant insulitis three weeks after the first streptozotocin-injection, were transferred into congenic thymusless C57BL/6J-nu/nu mice. The cell transfer led to lymphocytic infiltrations of pancreatic islets in 75% of the recipients. Hyperglycaemia was not observed. It is concluded that low-dose streptozotocin treatment induces cellular immune reactions against pancreatic islets.
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D001786:Blood Glucose / Q000032:analysis; D006943:Hyperglycemia / Q000276:immunology; D007515:Islets of Langerhans / Q000276:immunology*; D017710:Lymphocyte Transfusion; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010182:Pancreatic Diseases / Q000276:immunology*; D013154:Spleen / Q000637:transplantation*; D014184:Transplantation, Homologous
chemical_list: D001323:Autoantibodies; D001786:Blood Glucose