title: High-resolution phosphorus nuclear magnetic resonance spectra of yeast phenylalanine transfer ribonucleic acid. Metal ion effects and tentative partial assignment of signals.
abstract: The temperature and metal ion dependence of the 31P NMR spectra of yeast phenylalanine tRNA is presented, and a tentative assignment of some of the individual phosphate signals is offered. Signals A, C, F, P, and T have been assigned to the magnesium binding site of phosphates by monitoring the magnesium ion dependence in the 31P NMR spectra. In the presence of 10 mM Mg2+, specific paramagnetic line broadening effects are observed for signals A, D, E, and U upon addition of 0.001-0.1 Mn2+ ion per tRNA molecule. Through the combination of the above metal in experiments, X-ray studies, and earlier 1H and 31P NMR studies [particularly the tRNA modification 31P NMR experiments of Salemink et al. [Salemink, P..J.M., Swarthof T., & Hilbers, C.W. (1979) Biochemistry 18, 3477]], an initial assignment of some of these signals is attempted.
mesh_terms: D001483:Base Sequence; D008274:Magnesium; D009682:Magnetic Resonance Spectroscopy; D008345:Manganese; D009690:Nucleic Acid Conformation; D012346:RNA, Transfer, Amino Acyl; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D013696:Temperature
chemical_list: D012346:RNA, Transfer, Amino Acyl; D008345:Manganese; D008274:Magnesium