title: Curing of the 2 mu DNA plasmid from Saccharomyces cerevisiae.
abstract: The 2 mu DNA plasmid is often eliminated from yeast cells when they are transformed with the 2 mu DNA-LEU2-pMB9 composite plasmid pJDB219. Since pJDB219 is subsequently lost with high frequency, derivatives lacking all 2 mu DNA can be prepared from any strain.
mesh_terms: D004271:DNA, Fungal; D004274:DNA, Recombinant; D010957:Plasmids; D012093:Replicon; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal; D004274:DNA, Recombinant