title: Induction of labour in sheep after fetal hypophysectomy: an investigation of the possible involvement of a fetal pituitary secretion in the activation of placental enzymes by fetal cortisol.
abstract: Activities of 17 alpha-hydroxylase, C-17, 20 lyase and aromatase were measured in placentae of intact and hypophysectomized fetuses after premature induction of parturition by fetal administration of Synacthen. Activities of these enzymes were compared with concentrations of steroids produced by the placenta, which were measured in maternal plasma before and during parturition. Hypophysectomy was assessed by determining fetal plasma concentrations of LH before and after administering LH-RH, and histologically post partum. Concentrations of progesterone, which decreased before parturition, and of 17 alpha, 20 alpha-dihydroxypregn-4-en-3-one and oestradiol-17 beta, which increased, were not affected by fetal hypophysectomy. However, fetal hypophysectomy reduced the normal rise in oestrone sulphate which precedes parturition and abolished the normal rise in androstenedione. Although placental activities of C-17, 20 lyase and aromatase (but not 17 alpha-hydroxylase) tended to be reduced by fetal hypophysectomy, these effects were not statistically significant. The data support an earlier suggestion that an unidentified product of the fetal pituitary may be involved in the activation of C-17, 20 lyase by fetal cortisol before parturition. However, the results of the present study show that the action of such a factor is more likely to be permissive than obligatory.
mesh_terms: D000446:Aldehyde-Lyases / Q000097:blood*; D000818:Animals; D001141:Aromatase / Q000097:blood*; D004789:Enzyme Activation; D005260:Female; D005333:Fetus / Q000502:physiology*; D012739:Gonadal Steroid Hormones / Q000097:blood*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D006854:Hydrocortisone / Q000502:physiology; D006908:Hydroxyprogesterones / Q000097:blood*; D007016:Hypophysectomy; D007751:Labor, Induced; D007986:Luteinizing Hormone / Q000097:blood; D010088:Oxidoreductases / Q000097:blood*; D010902:Pituitary Gland / Q000502:physiology*; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D012756:Sheep; D013254:Steroid 17-alpha-Hydroxylase / Q000097:blood*; D013250:Steroid Hydroxylases / Q000097:blood*
chemical_list: D012739:Gonadal Steroid Hormones; D006908:Hydroxyprogesterones; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone; D010088:Oxidoreductases; D013250:Steroid Hydroxylases; D001141:Aromatase; D013254:Steroid 17-alpha-Hydroxylase; D000446:Aldehyde-Lyases; D006854:Hydrocortisone