Pyrimethamine sensitivity in Plasmodium falciparum: determination in vitro by a modified 48-hour test.
Four strains of Plasmodium falciparum recently isolated in culture were assessed in vitro for their response to pyrimethamine. A simple modified 48-hour test was used, which showed two strains to be sensitive to the drug in vitro, while the other two were resistant at a very high level. In the two strains for which relevant clinical information was available the in vivo response to pyrimethamine was corroborated by the in vitro findings. This modified 48-hour test is thus useful for determining patterns of drug sensitivity in laboratory-adapted strains, and would be a valuable asset if found to be equally applicable under field conditions.
D000328:Adult; D002478:Cells, Cultured; D004351:Drug Resistance; D005260:Female; D006801:Humans; D008288:Malaria / Q000097:blood / Q000188:drug therapy; D008875:Middle Aged; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000302:isolation & purification; D011739:Pyrimethamine / Q000494:pharmacology*
D011739:Pyrimethamine