Competitive enzyme immunoassay for human plasma fibronectin.
A competitive enzyme immunoassay for the determination of fibronectin in plasma is described. An enzyme conjugate prepared by coupling alkaline phosphatase to rabbit anti-human fibronectin antibodies by glutaraldehyde was used as principal reagent. The assay was performed by coating polystyrene tubes with purified fibronectin and reacting these coated tubes with a mixture of sample and enzyme-labeled antibodies. After overnight incubation, the amount of enzyme activity associated with the tube was determined. An assay range of 0.5-20 microgram/ml of fibronectin was obtained. The mean concentration of plasma fibronectin in female patients was found to be 270 microgram/ml (standard deviation 50 microgram/ml, n = 22). Denatured fibronectin had low activity in the assay. The presence of cross-reacting antigens in rat and guinea pig plasma was demonstrated by the enzyme immunoassay technique.
D000327:Adsorption; D000818:Animals; D000941:Antigens; D001667:Binding, Competitive; D002951:Citrates / Q000494:pharmacology; D003429:Cross Reactions; D005260:Female; D005353:Fibronectins / Q000097:blood*; D006168:Guinea Pigs; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D007700:Kinetics; D011817:Rabbits; D051381:Rats
D000941:Antigens; D002951:Citrates; D005353:Fibronectins; D007106:Immune Sera; D006493:Heparin