Human lymphocyte-activating properties of a purified polysaccharide from Candida albicans: B and T cell cooperation in the mitogenic response.
Purified human T and B lymphocytes were tested for their ability to show a mitogenic response to a purified polysaccharide (MPPS) extracted from C. albicans. It was found that neither T nor B cells alone could respond to MPPS. When a mixture of these two types of cells was tested, a normal response (comparable to the one of unfractionated peripheral blood lymphocytes) was observed. This response was not affected by prior depletion of macrophages. Results obtained with normal B or T cells mixed with mitomycin C-treated T or B cells indicated that a reciprocal helper effect exists between these two types of cells in their response to MPPS. This response could be inhibited by antisera against HL-A and Ia-like antigens.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002176:Candida albicans / Q000276:immunology*; D002448:Cell Adhesion; D002469:Cell Separation; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008285:Major Histocompatibility Complex; D008934:Mitogens / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D011134:Polysaccharides / Q000302:isolation & purification*; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology
D007106:Immune Sera; D008934:Mitogens; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D011134:Polysaccharides