[Reaction of sera from rabbits immunized with synthetic poly-L-alanine with myocardial interstitial connective tissue cells].
Sera from rabbits immunized with synthetic poly-L-alanine (PL-ala) in complete Freund's adjuvant (CFA) have been examined by the indirect immunofluorescence (IF) technique. Half of the sera reacted with fibroblasts of the interstitial connective tissue of the myocardium. The reactions were obtained with all sections of the myocardium of man and other mammals except rabbits. The sera obtained before or after immunization with synthetic poly-L-lysine fibroblasts were obtained with preparations of IgG or F(ab')2 fragments of IgG isolated from sera of animals immunized synthetic polypeptides can be applied to study by the IF technique of the antigenic structure of tissues including lymphoid and tumor tissues.
D000818:Animals; D001500:BCG Vaccine / Q000276:immunology; D005347:Fibroblasts / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005620:Freund's Adjuvant / Q000008:administration & dosage; D007106:Immune Sera / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D009206:Myocardium / Q000166:cytology / Q000276:immunology*; D010455:Peptides / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D011961:Receptors, Fc / Q000276:immunology
D001500:BCG Vaccine; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D010455:Peptides; D011961:Receptors, Fc; C019529:polyalanine; D005620:Freund's Adjuvant
Reaktsiia syvorotok krolikov, immunizirovannykh sinteticheskim poli-L-alaninom, s kletkami interstitsial'noĭ soedinitel'noĭ tkani miokarda.