Direct evaluation of class-specific anti-DNA antibodies by an immunoenzymatic technique.
Antibodies against native DNA have been evaluated by an immuno-enzymo assay (ELISA) using glucose oxidase labelled Ig class-specific antibodies. Results of the ELISA test were significantly correlated with those of the Farr test performed with native DNA as well as with the degree of clinical activity. ELISA is proposed as a simple method for the direct evaluation of class-specific anti-native DNA antibodies in systemic lupus and related diseases.
D000974:Antibodies, Antinuclear / Q000032:analysis*; D004247:DNA / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D009691:Nucleic Acid Denaturation
D000974:Antibodies, Antinuclear; D004247:DNA