In vitro conversion of proinsulin to insulin by cathepsin B in isolated islets and its inhibition by cathepsin B antibodies.
Purified bovine cathepsin B, when incubated with isolated rat islets of Langerhans, completely converts proinsulin to insulin as demonstrated by the incorporation of 14C-leucine into islet proteins, releasing lysine as the only basic amino acid. Cathepsin B antibodies raised in rabbit inhibited the above conversion.
D000818:Animals; D000906:Antibodies; D002401:Cathepsin B; D002403:Cathepsins / Q000276:immunology / Q000494:pharmacology*; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D007328:Insulin / Q000378:metabolism*; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D011384:Proinsulin / Q000378:metabolism*; D051381:Rats
D000906:Antibodies; D007328:Insulin; D011384:Proinsulin; D002403:Cathepsins; D002401:Cathepsin B