title: Studies on leakage of enzymes from washed bull and ram spermatozoa.
abstract: Washing of bull and ram spermatozoa resulted in significant losses of lactic dehydrogenase (LDH) and glucose phosphate isomerase (GPI) from the cell suspensions. Re-suspension of washed bull spermatozoa caused an immediate release of enzymes from the cells. Preincubation of washed ram spermatozoa with 0.025% formaldehyde increased GPI levels but decreased LDH concentration in the extracellular fluid while hexokinase release was unaffected. Varying the incubation temperature between 20 and 37 degrees C affected extracellular LDH and GPI levels. It is suggested that enzyme release from spermatozoa may occur in the absence of any apparent cellular damage.
mesh_terms: D000818:Animals; D002417:Cattle; D003584:Cytological Techniques; D005557:Formaldehyde / Q000494:pharmacology; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D012756:Sheep; D013094:Spermatozoa / Q000166:cytology / Q000201:enzymology*
chemical_list: D005557:Formaldehyde; D007770:L-Lactate Dehydrogenase; D005956:Glucose-6-Phosphate Isomerase