[Morphological studies of leukocyte long-term culture. 2. A new method for identification of proliferating tumor cells in peripheral blood].
The modified leucocyte-longterm culture is presented as adequate procedure for demonstration of tumor cells from peripheral blood of melanomalignoma patients. By means of the proliferation cinetics, an exact differentiation between tumor cells on one side, and melanophages, so called irritated monocytic forms and endothelial cells on the other side is possible. In this in vitro-system proliferative tumorcells from peripheral blood can be differed from non-proliferative ones. The interaction between tumor cells, macrophages and lymphocytes is reported.
D003470:Culture Media; D005549:Foreign-Body Reaction; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000378:metabolism / Q000648:ultrastructure*; D008545:Melanoma / Q000097:blood* / Q000175:diagnosis; D012397:Rosette Formation; D012878:Skin Neoplasms / Q000097:blood* / Q000175:diagnosis
D003470:Culture Media
Morphologische Untersuchungen in der Leukozyten-Lanzeitkultur. 2. Eine neue Nachweismethode für proliferationsaktive Tumorzellen aus dem peripheren Blut.