title: Photo-induced crosslinking between phenylalanine transfer RNA and messenger RNA on the Escherichia coli ribosome.
abstract: Crosslinking between phenylalanyl transfer RNA from brewer's yeast and poly(U) can be obtained when ribosomal complexes are irradiated at > 300 nm; the ribosomal complexes were formed by enzymatic binding of the tRNA. The crosslinked product was isolated on oligo(dA)-cellulose columns. The experiments suggest that the photo-crosslinking occurs via the wybutine in the anticodon loop of the tRNA. This system can be used to study interactions between tRNA and messenger RNA on the ribosome.
mesh_terms: D004926:Escherichia coli; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D008027:Light; D010649:Phenylalanine / Q000378:metabolism; D011072:Poly U / Q000378:metabolism; D012331:RNA, Fungal / Q000528:radiation effects*; D012333:RNA, Messenger / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism / Q000528:radiation effects*; D012270:Ribosomes / Q000378:metabolism / Q000528:radiation effects*; D012440:Saccharomyces
chemical_list: D012331:RNA, Fungal; D012333:RNA, Messenger; D011072:Poly U; C012099:wye; D010649:Phenylalanine; D006147:Guanine; D012343:RNA, Transfer