Further evaluation of indirect immunofluorescence methods for detection of antibodies against Aspergillus fumigatus.
The IF titres of IgG antibodies to hyphal antigen preparations of 6 isolates of Aspergillus fumigatus were compared with the titres to germling antigen preparations of the same isolates. Titres varied according the antigen used and the method of preparation. No single isolate or method of antigen preparation enabled a group of 15 patients with aspergillosis to be separated from a group of asthmatic subjects or a group of patients with other fungal infections, but significantly higher means IF titres were obtained for the group of patients with aspergillosis.
D000908:Antibodies, Fungal / Q000032:analysis*; D000946:Antigens, Fungal / Q000276:immunology; D001229:Aspergillosis, Allergic Bronchopulmonary / Q000150:complications / Q000276:immunology; D001232:Aspergillus fumigatus / Q000276:immunology*; D001249:Asthma / Q000150:complications / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D009181:Mycoses / Q000276:immunology
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D007074:Immunoglobulin G