[Accurate and rapid method of evaluating the activity of cholagogues in rats].
A precise and rapid method for quantitative and qualitative study of the activity of choleretic agents in rats is offered. The method involves the use of a preliminary calibrated transparent cannula tube 4 m long as a reservoir for hourly collection of the bile. The cannula tube is sewn into an isolated strip (sac) of anesthetized animals' duodenum supplied with the bile from the biliary duct. Ligation of the pancreatic duct prevents the supply of pancreatic juice to the sac. In order to restore the patency of the alimentary canal, the proximal and distal parts of the intestine are connected by a tube. The method was tried in experiments with the use of the test preparations insulin and cholongone. The method suggested can be used both for pharmacological screening and for the qualitative determination of the time course of choleresis.
D000818:Animals; D001646:Bile / Q000378:metabolism; D002756:Cholagogues and Choleretics / Q000494:pharmacology; D003685:Dehydrocholic Acid / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D007328:Insulin / Q000494:pharmacology; D051381:Rats; D013997:Time Factors
D002756:Cholagogues and Choleretics; D007328:Insulin; D003685:Dehydrocholic Acid
Tochnyĭ i bystryĭ metod otsenki aktivnosti zhelchegonnykh sredstv na krysakh.