Comparison of rat and guinea pig as sources of the S9 fraction in the Salmonella/mammalian microsome mutagenicity test.
A highly significant enhancement of mutagenicity occurs with 11 polycyclic aromatic hydrocarbons when 3-methylcholanthrene-induced guinea pig liver S9 is substituted for Aroclor-induced rat liver S9 in the Ames test. The use of MC-induced guinea pig liver S9 is particularly valuable for detecting the weak mutagenicity of benz[c]acridine, which is barely positive in a standard Ames assay. However, anthracene and phenanthrene, which are generally considered not to be carcinogens, remain non-mutagenic for strain TA100. This enhancement of mutagenicity does not correlate with arylhydrocarbon hydroxylase activities of the various liver preparations and does not apply to certain other non-PAH mutagens, including beta-naphthylamine, aflatoxin B1 and 4-dimethylaminoazobenzene.
D000166:Acridines / Q000494:pharmacology; D000818:Animals; D001711:Biotransformation; D005821:Genetic Techniques; D006168:Guinea Pigs / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism*; D009153:Mutagens; D051381:Rats / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000235:genetics*; D013045:Species Specificity
D000166:Acridines; D009153:Mutagens