title: On the promoter complex formation rate of E. coli RNA polymerases with T7 phage DNA.
abstract: Influence of ionic strength on the kinetics of the promoter complex formation between E. coli RNA polymerase and T7 phage DNA was investigated using a membrane filter assay. The enzyme-promoter association rate constant was determined. It varies from 10(9) to 3 x 10(7) M-1 sec-1 when the ionic strength is changed from zero to 0.15 M NaCl. Basing on the theoretical analysis of experimental data obtained the model for the promoter site selection assuming the enzyme sliding along the DNA is discussed.
mesh_terms: D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D008956:Models, Chemical; D009876:Operon / Q000187:drug effects; D009994:Osmolar Concentration; D013604:T-Phages / Q000378:metabolism*
chemical_list: D004279:DNA, Viral; D003609:Dactinomycin; D012321:DNA-Directed RNA Polymerases