Demonstration of PGI2 production by isolated perfused rat lungs with platelet aggregation test.
Platelet aggregation test was used for PGI2 measurements. The use of 6 % CO2 in air stabilized human platelet rich plasma (PRP) so that it could be used for up to 7 hours in these measurements. The PGI2 caused inhibition of aggregation in response to ADP was concentration dependent in the range of 0.5 ng/ml to 50 ng/ml. Isolated perfused rat lungs released spontaneously 190 ng/min PGI2 and about 3 % of infused arachidonic acid potassium salt (equivalent to 25 microgram/min arachidonic acid) was converted to PGI2.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology; D004355:Drug Stability; D011464:Epoprostenol / Q000096:biosynthesis* / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008168:Lung / Q000378:metabolism*; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D011453:Prostaglandins / Q000096:biosynthesis*; D051381:Rats
D001095:Arachidonic Acids; D011453:Prostaglandins; D000244:Adenosine Diphosphate; D011464:Epoprostenol