title: Calcium control of microfilaments: uncoupling of the F-actin-severing and -bundling activity of villin by limited proteolysis in vitro.
abstract: Villin is a major F-actin-bundling protein present in the microfilament bundle underlying the plasma membrane of the microvilli present on intestinal epithelial cells. Mild in vitro proteolysis converts villin (Mr, 95,000) into a large fragment, the villin core (apparent Mr, 90,000). Villin core has lost the F-actin-bundling activity expressed by villin in the absence of calcium but retains the micromolar Kd calcium-binding site and the calcium-dependent restriction of actin filament length (F-actin severing) of intact villin. This finding suggests a common structural and functional relatedness between the known calcium-dependent F-actin-severing proteins from different cell types, even though not all of them reveal F-actin-bundling activity.
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D002352:Carrier Proteins / Q000494:pharmacology*; D002417:Cattle; D002645:Chickens; D010450:Endopeptidases; D007700:Kinetics; D008840:Microfilament Proteins; D008854:Microscopy, Electron; D008970:Molecular Weight; D009132:Muscles / Q000378:metabolism; D011487:Protein Conformation; D012697:Serine Endopeptidases
chemical_list: D000199:Actins; D002352:Carrier Proteins; D008840:Microfilament Proteins; C026615:villin; D010450:Endopeptidases; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D002118:Calcium