Disappearance of nystatin resistance in Candida mediated by ergosterol.
Two nystatin-resistant mutants of Candida were isolated in vivo and identified as C. albicans and C. krusei. Analysis of the sterol composition of these cells indicated a total absence of ergosterol and an increased level of a possible precursor. Successive cultures of the resistant strains in a medium supplemented with 10 microgram ergosterol ml-1 induced in 5d a sensitivity to nystatin identical to that of the wild-type strain. These ergosterol-supplemented strains had an ergosterol content similar to that of the wild-type strain cultured in the absence of ergosterol. Resistance to nystatin was recovered after 5d subculture in medium without ergosterol.
D002175:Candida / Q000187:drug effects* / Q000235:genetics; D002176:Candida albicans / Q000187:drug effects; D004352:Drug Resistance, Microbial; D004875:Ergosterol / Q000494:pharmacology*; D009154:Mutation; D009761:Nystatin / Q000494:pharmacology*
D009761:Nystatin; D004875:Ergosterol