[Possibilities for automatic quantitative image analysis of cell organelles stained ultrahistochemically].
Metal-containing secretory granules of alpha 2-cells of the Langerhans' islets of normal and alloxandiabetic rats were stained for electron microscopy by a special method. Thereafter the electron micrographs were analysed by the Quantimet 720 image analysing computer with respect to size of the granules and their quantitative distribution in classes. The following statistical tests verified significant differences between the distributions of the granules under normal and diabetic conditions. The secretory granules from alloxandiabetic animals were diminished distinctly. The examination by image analysing computer confirmed objectively observations, we have made and published formerly.
D000818:Animals; D003201:Computers; D003584:Cytological Techniques; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D003921:Diabetes Mellitus, Experimental / Q000473:pathology; D006651:Histocytochemistry; D007515:Islets of Langerhans / Q000648:ultrastructure*; D051381:Rats; D013223:Statistics as Topic
Möglichkeiten der automatischen quantitativen Bildauswertung von ultrahistochemisch erfassten Zellorganellen.