Rat brain antigen concentration measured by 14C-labeled immunoprecipitates.
Newborn rats were labeled with [14C]glucose during the first 35 days of life. Specific radioactivity was the same in fibrin, hemoglobin and brain proteins. The radioactivity precipitated in specific immunoprecipitates obtained on crossed immunoelectrophoresis with polyspecific antisera was used to calculate the amounts of synaptosomal antigens. Immunoprecipitable D1, D2, and D3 amounted to approximately 3.0%, 2.2% and 2.4% of rat forebrain proteins respectively.
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000941:Antigens; D001921:Brain / Q000276:immunology; D002250:Carbon Radioisotopes; D005260:Female; D005947:Glucose; D007163:Immunosorbent Techniques; D008297:Male; D051381:Rats; D013570:Synaptic Membranes / Q000276:immunology*
D000941:Antigens; D002250:Carbon Radioisotopes; D005947:Glucose