Protection against non-enzymatic deacylation of Met-tRNA Met m by the ribosome : an approach to the measurement of aminoacyl-tRNA : ribosome affinity constants.
When bound to E. coli 70S ribosomes in the presence of poly (A, U, G) at 37 degrees C, Met-tRNA Met m is hydrolysed 20 times slower than when free in solution under the same conditions (lifetimes of 8.3 h and 22 min respectively). It is shown how this large difference in rate of hydrolysis may be used to study the equilibrium of aminoacyl-tRNA binding to ribosomes.
D004926:Escherichia coli / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D008433:Mathematics; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D012346:RNA, Transfer, Amino Acyl; C026464:tRNA(m)(Met), methionine-