Identification and functional characterization of monocytes in rheumatoid synovial fluid.
Mononuclear cell suspension from synovial fluids of 13 patients with rheumatoid arthritis and 5 patients with juvenile rheumatoid arthritis contained on average of 1.5 and 1.3% cytotoxic plaque-forming cells, respectively, when tested against a monolayer of antibody-sensitized sheep erythrocytes. The plaque-forming ability was completely abolished after removal of cells which had phagocytosed carbonyl iron. The highest proportion of plaque-forming cells was found in the cell suspensions that contained the highest percentage of peroxidase-positive mononuclear cells. The plaque-forming activity was almost completely inhibited by human IgG. Cell suspensions from patients treated with corticosteroids contained the highest proportions of plaque-forming cells and peroxidase-positive mononuclear cells compared with patients not receiving such treatment. Our findings indicate that the plaque-forming leukocytes in rheumatoid synovial fluid are monocytes.
D000305:Adrenal Cortex Hormones / Q000627:therapeutic use; D001171:Arthritis, Juvenile / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D002469:Cell Separation; D006098:Granulocytes; D006462:Hemolytic Plaque Technique; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D009000:Monocytes / Q000276:immunology*; D010586:Phagocytes; D011961:Receptors, Fc; D013582:Synovial Fluid / Q000166:cytology*
D000305:Adrenal Cortex Hormones; D011961:Receptors, Fc; D006821:Hyaluronoglucosaminidase