title: Modified granulocyte test for determination of mannosidosis genotype of cattle.
abstract: Recovery of alpha-mannosidase, beta-N-acetylglucosaminidase and arylsulphatase activity from granulocytes is increased by inclusion of 154 mM sodium chloride, in place of 1 mM zinc chloride, in aqueous Triton X-100 used to extract the cells. Calculation of the ratio of alpha-mannosidase to beta-N-acetylglucosaminidase and to arylsulphatase permits diagnosis of the mannosidosis genotype without recourse to examination of smears of the granulocyte preparations.
mesh_terms: D000118:Acetylglucosaminidase / Q000097:blood; D000818:Animals; D001192:Arylsulfatases / Q000097:blood; D002239:Carbohydrate Metabolism, Inborn Errors / Q000175:diagnosis / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D002712:Chlorides; D004796:Clinical Enzyme Tests / Q000662:veterinary*; D006098:Granulocytes / Q000201:enzymology*; D012965:Sodium Chloride / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology; D017967:Zinc Compounds; D000520:alpha-Glucosidases / Q000097:blood
chemical_list: D002712:Chlorides; D017967:Zinc Compounds; D012965:Sodium Chloride; C016837:zinc chloride; D001192:Arylsulfatases; D000520:alpha-Glucosidases; D000118:Acetylglucosaminidase; D015032:Zinc