Haemophilus influenzae type b capsular polysaccharide detection and measurement by an enzyme-linked immunosorbent assay (ELISA).
A direct sandwich enzyme-linked immunosorbent assay (ELISA) was developed to detect Haemophilus influenzae type b capsular antigen. Using polystyrene as the solid phase and peroxidase-labelled rabbit antibody the assay detected the antigen in concentrations of 0.1 ng/ml. Linearity was achieved within the range of 1ng to 10 microgram/ml. Subtle measurements of Haemophilus influenzae type b capsular antigen in body fluids are possible through ELISA which is superior to counterimmunoelectrophoresis and latex-particle agglutination in this respect. ELISA should facilitate investigations concerning PRP pathogenic effects in experimental Hib infection as well as in human Hib disease.
D001769:Blood / Q000382:microbiology; D002473:Cell Wall / Q000276:immunology; D002555:Cerebrospinal Fluid / Q000382:microbiology; D003378:Counterimmunoelectrophoresis; D004797:Enzyme-Linked Immunosorbent Assay; D006192:Haemophilus Infections / Q000175:diagnosis; D006193:Haemophilus influenzae / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007841:Latex Fixation Tests; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D014556:Urine / Q000382:microbiology
D011135:Polysaccharides, Bacterial