A method for the analysis of catecholamines by selected ion monitoring and 14C isotope dilution in adrenal medullary cell culture.
Extracts have been made from culture medium of rat medullar adrenal cells developed in tissue culture in this laboratory. After pentafluorobenzylimine-trimethylsilyl ether formation the catecholamine derivatives were characterized by gas-liquid chromatography chemical ionization mass spectrometry. In order to assess the catecholamine production capabilities of the cells in culture, a mass spectrometric method with isotope dilution has been devised. Chemical ionization selected ion monitoring allows specific detection at the nanogram level in a higher mass range (400-600 amu) than in the electron impact mode. The isotopic dilution method with 14C catecholamines gives rise to accurate measurements and linear response in the picomole range. The use of the [M-15]+ ion for monitoring m/z values minimizes errors in selected ion monitoring analysis. The results obtained are computerized and treated by the data system for fine background subtraction when high sensitivity and accuracy are required.
D000313:Adrenal Medulla / Q000032:analysis*; D000818:Animals; D002395:Catecholamines / Q000032:analysis*; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D013058:Mass Spectrometry; D011865:Radioisotope Dilution Technique; D051381:Rats
D002395:Catecholamines