A mutant of E. coli that restricts growth of bacteriophage T4 at elevated temperatures.
After nitrosoguanidine mutagenesis, a Phage Host Defective (phd) mutant of E. coli HfrH was isolated that supported the growth of T4D wild-type bacteriophage at 30 degrees, but not at 40 degrees or higher. Eleven independent spontaneous mutants of T4 (go mutants) were isolated that overcame the growth restriction at high temperature. All of these mutants were located within three percent recombination of a gene 39 amber mutation in the clockwise direction on the standard map. In mixed infections, the representative go mutant chosen for further study seems to be recessive to its wild-type allele. Temperature-shift experiments suggested that the mutated host function involved in phage growth is a "late" function, beginning in mid-eclipse.--Electrophoresis of phage proteins labelled early and late in infection showed that under restrictive conditions early protein synthesis was normal, but that certain late proteins were absent. However, measurements of DNA synthesis showed that under restrictive conditions the amount of phage DNA synthesized, and especially the amount of DNA sedimenting as high molecular weight replicative intermediate, was reduced. Pulse-chase experiments showed that the phage DNA made under restrictive conditions was not rapidly degraded.
D002213:Capsid / Q000096:biosynthesis; D002499:Centrifugation, Density Gradient; D002874:Chromosome Mapping; D003090:Coliphages / Q000254:growth & development*; D004269:DNA, Bacterial; D004279:DNA, Viral / Q000096:biosynthesis; D004586:Electrophoresis; D004926:Escherichia coli / Q000235:genetics*; D006358:Hot Temperature; D008769:Methylnitronitrosoguanidine; D009154:Mutation
D004269:DNA, Bacterial; D004279:DNA, Viral; D008769:Methylnitronitrosoguanidine