Evidence for the non-peptide nature of neutrophil-derived eosinophil chemotactic factor.
Neutrophil-derived low molecular weight eosinophil chemotactic factor (ECFPMN) was examined by a number of methods to prove its peptide nature. Sephadex G-10 eluates of ECFPMN were analyzed by paper chromatography and thin-layer chromatography, and peptides were separated on a peptide analyzer. Peptides isolated by these three different methods have no neutrophil or eosinophil chemotactic activity over a wide range of concentrations. Enzyme digestion studies supported these findings. Other physical and biological properties of ECFPMN suggest that this material is most likely lipid in nature.
D002630:Chemotactic Factors / Q000032:analysis*; D008055:Lipids / Q000032:analysis; D009504:Neutrophils / Q000276:immunology; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000032:analysis*
D002630:Chemotactic Factors; D008055:Lipids; D010455:Peptides; D010447:Peptide Hydrolases