Detection of mouse cells producing antibodies against the azophenylarsonic group by haemolytic plaque assay.
For detection of mouse cells producing anti-ARS antibody a modified method using ARS-SRBC is described. For optimal results a suitable source of SRBC must be selected. The number of ARS groups per SRBC required for optimal lysis in PFC assay varies, and ARS-SRBC prepared by the method of Ingraham which are suitable for detection of rabbit-PFC give negative results with the mouse system. ARS-SRBC prepared for the PFC assay are unsuitable for the haemagglutination test and vice versa.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D001391:Azo Compounds / Q000276:immunology*; D001667:Binding, Competitive; D002417:Cattle; D004136:Dinitrobenzenes / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D006461:Hemolysis; D006462:Hemolytic Plaque Technique; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H; D011817:Rabbits; D012709:Serum Albumin / Q000276:immunology; D012756:Sheep; D013552:Swine; D010132:p-Azobenzenearsonate / Q000276:immunology*
D001391:Azo Compounds; D004136:Dinitrobenzenes; D007140:Immunoglobulin Fab Fragments; D012709:Serum Albumin; D010132:p-Azobenzenearsonate