Identification of cells expressing T and p28,33 (Ia-like) antigens in sections of human lymphoid tissue.
A method has been developed for identifying human lymphocyte populations in tissue sections using hetero-antisera to T cells and to the B cell associated Ia-like, p28,33 polypeptide complex. The differential topographical localisation of T and B cells is revealed by simultaneous staining with antibodies tagged with different fluorochromes; this approach also identifies a small population of T cells bearing Ia-like antigens. Preliminary studies on lymphomatous tissue suggests this approach will be of value in the study of malignancy and cellular infiltration in disease.
D000954:Antigens, Surface / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D002648:Child; D005455:Fluorescent Antibody Technique; D006649:Histocompatibility Antigens / Q000032:analysis*; D006801:Humans; D007223:Infant; D007945:Leukemia, Lymphoid / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology; D008223:Lymphoma / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D006649:Histocompatibility Antigens