Growth-mediated metabolic activation of promutagens in Aspergillus nidulans.
7 procarcinogens belonging to different chemical classes (nitrosamines, hydrazoalkanes, oxazaphosphorines and aromatic amines) were tested in A. nidulans for the induction of point mutations with two genetic systems (8-AG resistance and induction of methionine suppressors). Dimethylnitrosamine, diethylnitrosamine, nitrosomorpholine, dimethylhydrazine, procarbazine and cyclophosphamide gave positive results with a good dose--effect relationship in the growth-mediated assay, whereas they gave negative or borderline positive results in the plate incorporation assay. 2-Aminoanthracene was completely negative with both experimental procedures. DMN, DEN and NM were also tested for their ability to induce somatic segregation: all were positive when assayed in the growth-mediated assay.
D000588:Amines / Q000494:pharmacology; D001233:Aspergillus nidulans / Q000235:genetics*; D002273:Carcinogens / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006834:Hydrazines / Q000494:pharmacology; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens; D009154:Mutation; D009602:Nitrosamines / Q000494:pharmacology
D000588:Amines; D002273:Carcinogens; D006834:Hydrazines; D009153:Mutagens; D009602:Nitrosamines