title: Effect of alloxan on insulin and glucagon secretion in perifused isolated islets.
abstract: The effect of alloxan on insulin and glucagon secretion was investigated in perifused isolated rat islets. Five minutes of exposure to 1.4 mmol/l alloxan in a low-glucose medium (5.6 mmol/l) abolished subsequent leucine stimulated insulin and glucagon secretion. In a medium containing 19 mmol/l arginine and 3.3 mmol/l glucose, insulin secretion was only slightly diminished by alloxan pretreatment, whereas glucagon secretion was reduced to about 60% of controls. Exposure to alloxan in a high glucose medium (27.8 mmol/l) did not effect insulin or glucagon secretion.
mesh_terms: D000496:Alloxan / Q000494:pharmacology*; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D005934:Glucagon / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007930:Leucine / Q000494:pharmacology; D008297:Male; D010477:Perfusion; D051381:Rats
chemical_list: D007328:Insulin; D000496:Alloxan; D005934:Glucagon; D001120:Arginine; D007930:Leucine; D005947:Glucose