title: [The role of chemical synthesis of DNA in gene technology (author's transl)].
abstract: A review of applications of chemically synthesized oligodeoxynucleotides to gene technology is given. At first the reader is briefly introduced to the methods of oligodeoxynucleotide synthesis. With selected examples the utilization of synthetic oligodeoxynucleotides is discussed in some detail for sequencing DNA and RNA, for the incorporation of base analogs and sequence specific mutations into nucleic acids of natural origin, for the isolation of specific nucleic acid sequences from biological sources, for the specific inhibition of virus reproduction, as linker molecules for gene cloning experiments and for the total synthesis of signal structures (regulatory sequences) and structural genes. The importance of synthetic oligodeoxynucleotides for molecular biology and gene technology especially is emphasized.
mesh_terms: D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000138:chemical synthesis*; D005818:Genetic Engineering; D009154:Mutation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000494:pharmacology; D011506:Proteins / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects
chemical_list: D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D011506:Proteins; D004247:DNA
vernacular_title: Die Bedeutung der chemischen Synthese von DNS für die Gentechnologie.