[Increase in yeast and bacterial sensitivity to inhibitors and riboflavin as affected by high sulfate and phosphate concentrations].
Cultivation of the yeast Pichia guilliermondii in a medium with a high content of sulfate or phosphate ions (0.6 M and higher) increased its susceptibility to actinomycin D and 7-methyl-8-trifluoromethyl 10-(1'-D-ribityl)isoalloxazin, and analog of riboflavin, and decreased the requirement of the riboflavin-dependent mutant P7 in exogenous vitamin B2. The protoplasts of the yeast were also very susceptible to actinomycin D when they were incubated in a medium with a high sulfate concentration. Sulfate and phosphate ions elevated the susceptibility to actinomycin D in the following yeasts, apart from P. guilliermondii: Pichia pinus, Saccharomyces cerevisiae, Torulopsis candida, hansenula polymorpha, Schwanniomyces occidentalis, Candida utilis and Candida tropicalis. The growth of Escherichia coli was also very susceptible to actinomycin D when the bacterium was cultivated in medium with an elevated phosphate concentration (0.2 M). High phosphate or sulfate concentrations can be used in experiments aimed at studying the effect of transcription inhibitors (actinomycin D, 8-hydroxyquinoline) on the induction of alpha-glucosidase in P. guilliermondii.
D003470:Culture Media; D003609:Dactinomycin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D010710:Phosphates / Q000008:administration & dosage*; D012256:Riboflavin / Q000494:pharmacology*; D013431:Sulfates / Q000008:administration & dosage*; D015003:Yeasts / Q000187:drug effects*
D003470:Culture Media; D010710:Phosphates; D013431:Sulfates; D003609:Dactinomycin; D012256:Riboflavin
Uvelichenie chuvstvitel'nosti drozhzheĭ i bakteriĭ k nekotorym ingibitoram i riboflavinu pod vozdeĭstviem vysokikh kontsentratsiĭ sul'fata i fosfata.