Temperature-dependent staining reaction of chromatin by Alcian blue.
After treatment with Alcian blue at high temperatures, nuclei from chicken blood smears showed an intense staining reaction, which proved to be dependent on the DNA content of chromatin. The possibility that interactions between the phthalocyanine chromophore and unpaired bases accounts for this temperature-dependent staining of chromatin is briefly discussed.
D000423:Alcian Blue; D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000032:analysis*; D004247:DNA / Q000097:blood*; D004912:Erythrocytes / Q000032:analysis; D006652:Histological Techniques; D013696:Temperature
D002843:Chromatin; D004247:DNA; D000423:Alcian Blue