Production of group C streptococcus phage-associated lysin and the preparation of Streptococcus pyogenes protoplast membranes.
Optimum conditions of pH, temperature and multiplicity of bacteriophage infection for the production of phage-associated lysin from Group C streptococci were defined. Lysin obtained by the optimum method was used to make protoplast membranes of Group A type 12 streptococci. These membranes consistently contained very low concentrations of rhamnose, indicating minimal cell wall contamination. The development of such a reproducible technique for the preparation of protoplast membranes will permit more meaningful studies on the involvement of these membranes in the pathogenesis of acute post-streptococcal glomerulonephritis.
D001433:Bacteriolysis; D001435:Bacteriophages; D002458:Cell Fractionation; D002462:Cell Membrane; D003470:Culture Media; D004798:Enzymes; D006863:Hydrogen-Ion Concentration; D011523:Protoplasts; D013291:Streptococcus / Q000201:enzymology*; D013297:Streptococcus pyogenes / Q000648:ultrastructure*; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D004798:Enzymes; C028806:lysin, streptococcus bacteriophage