Insulin effect on lung subcellular fraction phospholipids and cholesterol.
Lung subcellular fraction (microsomes, mitochondria, 105 000 X g supernatant and plasma membranes) were isolated from normal and insulin treated rats. Phospholipid and cholesterol contents were determined, phosphatidylcholines and phosphatidyl-ethanolamines separated and their fatty acid profiles assayed. Teh investigations carried out indicated that insulin influenced the phospholipid content of all fractions. Cholesterol levels in the 105 000 X g supernatant and especially in the plasma membrane fraction were raised significantly. Insulin administration increased the contribution of the unsaturated long chain fatty acids in both phospholipids studied. The results are discussed in relation to stimulated lipid biosynthesis and to lipid fluidity, of which fatty acyl unsaturation and cholesterol level are main parameters.
D000818:Animals; D002784:Cholesterol / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism*
D005227:Fatty Acids; D007328:Insulin; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D002784:Cholesterol