Induction of mitotic recombination by certain hair-dye chemicals in Saccharomyces cerevisiae.
A number of procedures were used to test for the potential of 5 hair-dye chemicals, 4-nitro-o-phenylenediamine, 2-nitro-p-phenylenediamine, m-phenylenediamine 2,4-diaminoanisole sulfate and 2,5-diaminoanisole sulfate, to induce genetic damage in yeast strains D3 and D4 of Saccharomyces cerevisiae. Various plate-test procedures, short-term suspension assays in phosphate buffer and suspension assays with liver enzyme activation all proved to be ineffective for demonstrating genetic effects of these chemicals. Only suspension assays in which the yeast cells were treated with the test chemical under growing conditions for up to 72 h were effective in demonstrating the genetic activity of 4-nitro-o-phenylenediamine and 2,4-diaminoanisole sulfate. The implications of these results for testing of mutagens in yeast systems are discussed along with other supportive evidence from the literature.
D003470:Culture Media; D006202:Hair Dyes / Q000494:pharmacology*; D006203:Hair Preparations / Q000494:pharmacology*; D008938:Mitosis; D009152:Mutagenicity Tests; D009153:Mutagens; D010655:Phenylenediamines / Q000494:pharmacology*; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics
D003470:Culture Media; D006202:Hair Dyes; D006203:Hair Preparations; D009153:Mutagens; D010655:Phenylenediamines