Anti-choline acetyltransferase fragments antigen binding (Fab) for immunohistochemistry.
The preparation of fragments antigen binding (Fab) from serum of rabbits immunized against human brain choline acetyltransferase (CAT) is described. Ant-CAT Fab fragments were less potent than anti-CAT immunoglobulin (IgG) in inhibiting mammalian CAT activity and did not precipitate CAT activity. They proved, however, far superior to anti-CAT IgG for the immunohistochemical staining of CAT in mammalian neurons.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002415:Cats; D002645:Chickens; D002795:Choline O-Acetyltransferase / Q000032:analysis / Q000276:immunology*; D006168:Guinea Pigs; D006651:Histocytochemistry; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007163:Immunosorbent Techniques; D011817:Rabbits / Q000276:immunology; D051381:Rats
D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D002795:Choline O-Acetyltransferase