Monoclonal antibodies to murine cell surface antigens. I. Lyt-1.1.
The production and properties of a monoclonal anti-Lyt-1.1 antibody are described. The monoclonal antibody is of the IgG2a subclass, is cytotoxic, and reacts with Staphylococcal protein A. The tissue culture supernatant has the same activity (titer on thymus > 1/1000) as an anti-Lyt-1.1 produced by a standard immunization procedure, and after in vivo passage of the hybridoma cell line, the serum titer was > 1:10(6). The monoclonal antibody has the same strain and tissue distribution as the conventional antibody, including reactions on Lyt-1 congenic strains, and it precipitates a cell surface molecule of m.w. approximately 67,000. In nonprimed mice, there were similar numbers of Thy-1+ and Lyt-1+ cells in spleen, lymph node, and thymus. By using the monoclonal reagent, T-helper and T-killer cells in an allogenic system were shown to be Lyt-1.1.
D000818:Animals; D000954:Antigens, Surface; D001402:B-Lymphocytes / Q000276:immunology; D002459:Cell Fusion; D002999:Clone Cells / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D007106:Immune Sera / Q000494:pharmacology; D007158:Immunologic Techniques; D007518:Isoantibodies / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013601:T-Lymphocytes / Q000276:immunology
D000954:Antigens, Surface; D007106:Immune Sera; D007518:Isoantibodies