Sephadex G-10 columns do not retain selectively T or B lymphocyte subpopulations.
The passage through Sephadex G-10 columns of lymphocytes prepared from different lymphoid compartments of man, guinea pig and rabbit, did not result in a selective loss of lymphocyte subsets, as assessed by various rosetting assays. This lack of T and B lymphocyte loss was seen irrespective of whether glass beads or a fine mesh net was used as a support for the Sephadex G-10 column.
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D002469:Cell Separation / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D006168:Guinea Pigs; D006801:Humans; D007158:Immunologic Techniques; D007958:Leukocyte Count; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000276:immunology; D014066:Palatine Tonsil / Q000166:cytology; D011650:Pulmonary Alveoli / Q000166:cytology; D011817:Rabbits; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000166:cytology*