A simple and rapid procedure for the large scale purification of the recA protein of Escherichia coli.
A simple and rapid three-step procedure for the large scale purification of the recA protein of Escherichia coli is described. The method depends primarily on a single chromatographic step which is highly specific for recA protein: elution by ATP from single-stranded DNA cellulose. With this procedure, gram quantities of recA protein, greater than 99% pure, can be reproducibility prepared for biochemical and biophysical analysis.
D000645:Ammonium Sulfate; D001426:Bacterial Proteins / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004926:Escherichia coli / Q000235:genetics*; D011938:Rec A Recombinases; D011995:Recombination, Genetic
D001426:Bacterial Proteins; D011938:Rec A Recombinases; D000645:Ammonium Sulfate