Metabolizing activities of postmitochondrial liver fractions (S-9) on benzo[a]pyrene and dimethylnitrosamine.
The mutagenic activity of the carcinogens benz[a]pyrene and dimethylinitrosamine after being metabolised by postmitochondrial liver fractions (S-9) from rats, Syrian hamsters and rabbits was estimated in the Ames' test. The liver donors were pretreated with phenobarbital, 3-methylcholanthrene, PCB (Delor 106) or peanut oil. Parallel to the mutagenicity tests, enzymes of S-9 such as dimethylnitrosamine demethylase and aryl hydrocarbon hydroxylase were estimated and their activity evaluated with reference to the results of the Ames' test.
D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism*; D006224:Cricetinae; D004128:Dimethylnitrosamine / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008647:Mesocricetus; D011817:Rabbits; D051381:Rats; D012306:Risk; D012486:Salmonella typhimurium / Q000235:genetics; D013045:Species Specificity; D013347:Subcellular Fractions / Q000378:metabolism
D001580:Benzopyrenes; D004128:Dimethylnitrosamine