Hormonal requirements for growth of arterial smooth muscle cells in vitro: and endocrine approach to atherosclerosis.
In this study the hormonal requirements for the growth of arterial smooth muscle cells in vitro were determined. A serum-free, biochemically defined medium, supplemented with the relevant hormones, permitted proliferation and propagation of normal diploid mammalian arterial smooth muscle cells. Serum-free, hormone-supplemented cultures spontaneously formed atherosclerotic plaque-like nodules. Thus atherosclerosis may be mediated by a complex endocrine system.
D000818:Animals; D001012:Aorta, Abdominal / Q000166:cytology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D006133:Growth Substances / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D051381:Rats; D014168:Transferrin / Q000494:pharmacology
D003470:Culture Media; D006133:Growth Substances; D006728:Hormones; D007328:Insulin; D014168:Transferrin