[Serological diagnosis of human brucellosis by means of an immunoenzymatic technic (ELISA). II.--Comparison with four other usual reactions (author's transl)].
The authors analysing 553 serums, compare the results given by ELISA, in the diagnosis of human brucellosis, with four other usual serological tests : agglutination, rose Bengal plate agglutination test, complement fixation test, immunofluorescence. In the ELISA standard study and the research of specific M component, they can estimate, experimentally, the minimal absorbance necessary for concluding in positivity. They show the excellent specificity and sensitivity of this assay, its performances are almost the same as the immunofluorescence ones. Among 55 positive serums in one assay at least 83,6% are positive in both tests ELISA and IIF and 90,9% are detected by ELISA alone.
D002006:Brucellosis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D012698:Serologic Tests / Q000379:methods
Application de la technique immunoenzymatique ELISA (enzyme-linked-immunosorbent-assay) au diagnostic sérologique des brucelloses humaines. II.--Comparaison aux autres techniques sérologiques existantes.