Amplification of the biotin-avidin immunmofluorescence technique.
An amplification of the immunofluorescence technique which uses biotinylated antibody and fluoresceinated avidin is described. By introducing a sandwich technique using fluorescein-conjugated goat anti-avidin, a 5-fold enhancement of staining over the conventional immunofluorescence method is achieved, and the brightness is more than twice that achieved with the simple biotin-fluoresceinated avidin reaction.
D000818:Animals; D001360:Avidin; D001710:Biotin; D005455:Fluorescent Antibody Technique; D008722:Methods; D051379:Mice; D010047:Ovalbumin / Q000031:analogs & derivatives*; D011817:Rabbits
D001360:Avidin; D001710:Biotin; D010047:Ovalbumin