title: Intracellular distribution of carbon dioxide-fixing enzymes in Trypanosoma cruzi and Crithidia fasciculata.
abstract: The intracellular distribution of phosphoenopyruvate carboxykinase (EC 4.1.1.49) and NADP-linked malic enzyme (EC 1.1.1.40) activity in epimastigotes of Trypanosoma cruzi (Tulahuén strain) and in Crithidia fasciculata has been studied by two procedures: (i) subcellular fractionation by differential centrifugation of homogenates obtained by breaking the cells in a mortar; (ii) selective disruption of cellular membranes by digitonin treatment. Phosphoenolpyruvate carboxykinase is particulate in both organisms, as is one of the two forms of malic enzyme present in T. cruzi (malic enzyme I), whereas the other malic enzyme of T. cruzi (malic enzyme II) and the single malic enzyme of C. fasciculata are in the cytosol.
mesh_terms: D002950:Citrate (si)-Synthase / Q000378:metabolism; D003421:Crithidia / Q000201:enzymology*; D008291:Malate Dehydrogenase / Q000378:metabolism*; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism*; D013347:Subcellular Fractions / Q000201:enzymology; D014349:Trypanosoma cruzi / Q000201:enzymology*
chemical_list: D008291:Malate Dehydrogenase; D002950:Citrate (si)-Synthase; D010729:Phosphoenolpyruvate Carboxykinase (GTP)