[An immunohistochemical study of HCG and HPL on functional differentiation of human chorionic villi (author's transl)].
The enzyme-labeled antibody method was used to examine the localized distribution and variation by gestation week of HCG and HPL in placenta of 44 cases. 1) HCG: Electron microscopy revealed HRP reaction products in cisterna and membrane of perinuclear spaces and endoplasmic reticulum of syncytial cell (S-cell), but not in surface of S-cell microvilli or in Langhans' cell (L-cell). HRP reaction products in S-cell were more appreciable during 5th-12th weeks of gestation in syncytial sprouts, junction of villi with decidua, and rami or ramuli chorii, and fewer after 12th weeks. 2) HPL: Electron microscopy revealed reaction products in nuclear membrane and membrane of endoplasmic reticulum and Golgi complex, but not in surface of S-cell microvilli or L-cell. Reaction products in S-cell were noted from 8th gestation week, and most appreciably in 16th-18th gestation weeks preceding peak HPL concentration in serum. This indicates HPL exists in S-cell in the from of Pre. Pro-HPL.
D006063:Chorionic Gonadotropin / Q000032:analysis* / Q000276:immunology; D002824:Chorionic Villi / Q000032:analysis* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D010920:Placenta / Q000032:analysis*; D010928:Placental Lactogen / Q000032:analysis* / Q000276:immunology; D011247:Pregnancy
D006063:Chorionic Gonadotropin; D010928:Placental Lactogen