title: Membrane proteins associated with amino acid transport by yeast (Saccharomyces cerevisiae).
abstract: Cells of the wild-type yeast (Saccharomyces cerevisiae) strain Y185, grown under conditions that de-repress the formation of a general amino acid permease ('Gap') system, bind delta-N-chloroacetyl[1-(14)C]ornithine; L- and D-amino acid substrates of the general amino acid permease system protect against this binding. The protein responsible is released from the cells by homogenization or by preparation of protoplasts; it is not released by osmotic shock. This protein is virtually absent from the wild-type strain when it is grown under conditions that repress the general amino acid permease system, and is also absent from a Gap- mutant Y185-His3, selected by its resistance to D-amino acids. This mutant and repressed wild-type cells also fail to form a number of membrane proteins elaborated by de-repressed wild-type cells. It is possible that all these proteins are components of the general amino acid permease system.
mesh_terms: D000596:Amino Acids / Q000378:metabolism* / Q000494:pharmacology; D001692:Biological Transport; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000378:metabolism*; D009154:Mutation; D009952:Ornithine / Q000031:analogs & derivatives / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014364:Tryptophan / Q000378:metabolism
chemical_list: D000596:Amino Acids; D008565:Membrane Proteins; C026946:N-delta-chloroacetyl-L-ornithine; D014364:Tryptophan; D009952:Ornithine