title: Isolation and some characteristics of urokinase inhibitors isolated from pig leucocytes.
abstract: Two inhibitors of urokinase were isolated from the extracts of the nuclei of peripheral pig leucocytes, by ion exchange chromatography and by gel filtration on Sephadex G-100 and G-150. Only the inhibitor with the higher molecular weight was isolated in the homogenous form, in a single polypeptide chain with molecular weight of 68,000, designated Inhibitor-3. This inhibitor has an isoelectric point from 4.4 to 4.5 and is stable in buffer solutions from pH 3 to 8, and belongs to the type of so-called fast reacting inhibitors.
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003956:Dialysis; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007962:Leukocytes; D008970:Molecular Weight; D009994:Osmolar Concentration; D010447:Peptide Hydrolases / Q000494:pharmacology; D011480:Protease Inhibitors; D013552:Swine; D014568:Urokinase-Type Plasminogen Activator / Q000037:antagonists & inhibitors*
chemical_list: D011480:Protease Inhibitors; D010447:Peptide Hydrolases; D014568:Urokinase-Type Plasminogen Activator