title: Variability in generation of anti-sheep erythrocyte plaque-forming cells from cultures of human peripheral blood. Influence of monocytes.
abstract: We have encountered two types of variability in the numbers of cells making antibodies in vitro to sheep erythrocytes (PFC) in pokeweed-stimulated human peripheral blood mononuclear lymphocytes (PBM). One is a variation in number of PFC generated by the PBM of one individual compared -o that of another, which is probably an in vitro counterpart to the well known individual variation in in vivo antibody responsiveness and presumed to be T cell controlled. The other variability is in numbers of PFC found in replicate tubes from a given individual. This variation is not T cell controlled, but rather is releated to the relative number of monocytes in the system. Enhancement by monocytes of the numbers of PFC generated is also demonstrated.
mesh_terms: D000818:Animals; D000921:Antibody-Producing Cells / Q000145:classification* / Q000276:immunology; D001782:Blood Donors; D002469:Cell Separation; D004912:Erythrocytes / Q000145:classification / Q000276:immunology*; D005362:Ficoll; D006462:Hemolytic Plaque Technique; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000145:classification / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D011043:Pokeweed Mitogens / Q000276:immunology; D012756:Sheep / Q000097:blood / Q000276:immunology*
chemical_list: D011043:Pokeweed Mitogens; D005362:Ficoll