Substrate channeling: alpha-ketobutyrate inhibition of acetohydroxy acid synthase in Salmonella typhimurium.
Excess alpha-ketobutyrate inhibited the growth of Salmonella typhimurium LT2 by inhibiting the acetohydroxy acid synthase-catalyzed synthesis of alpha-acetolactate (a valine precursor). As a result, cells were starved for valine, and both ilvB (encoding acetohydroxy acid synthase I) and ilvGEDA (ilvG encodes acetohydroxy acid synthase II) were derepressed. The addition of valine reversed the effects of alpha-ketobutyrate.
D000095:Acetolactate Synthase / Q000037:antagonists & inhibitors* / Q000235:genetics; D002087:Butyrates / Q000494:pharmacology*; D007651:Keto Acids / Q000494:pharmacology; D009154:Mutation; D007652:Oxo-Acid-Lyases / Q000037:antagonists & inhibitors*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D014633:Valine / Q000494:pharmacology
D002087:Butyrates; D007651:Keto Acids; C005087:alpha-ketobutyric acid; D000095:Acetolactate Synthase; D007652:Oxo-Acid-Lyases; D014633:Valine