Structure of the peptidoglycan-associated lipoprotein (PAL) of the Proteus mirabilis outer membrane. II. Sequence of the amino-terminal part of the peptidoglycan-associated lipoprotein.
The amino acid of the lipooligopeptide (LOP) derived from the N-terminal region of the peptidoglycan-associated lipoprotein (PAL) of Proteus mirabilis was determined to be [X]-Ser-Ser-Asn-Lys. An unidentified compound[X] present at the N-terminus was identified as glycerylcysteine [S-(propane-2,'3'-diol)-3-thio, 2-amino-propanoic acid]. The partial amino acid sequence of the lipopolypeptide (LPP), which contained the lipooligopeptide (LOP) at its N-terminal part, was also determined, mainly by Edman-degradation. The structure of the N-terminal part of PAL was determined to be [3 Fatty acids approximately glycerylcysteine-Ser-Ser-Asn-Lys-Asn-Asp-Asp-Glu-Thr-Asp-Thr-Ser...]. The structure of PAL is discussed in comparison with Braun's lipoprotein.
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins; D043424:Carboxypeptidase B; D002268:Carboxypeptidases / Q000378:metabolism; D043422:Carboxypeptidases A; D003545:Cysteine / Q000031:analogs & derivatives / Q000032:analysis; D029968:Escherichia coli Proteins; D005227:Fatty Acids / Q000032:analysis; D008074:Lipoproteins / Q000032:analysis*; D010457:Peptidoglycan / Q000032:analysis*; D011509:Proteoglycans; D011513:Proteus mirabilis / Q000032:analysis*
D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; C031163:ExcC protein, E coli; D005227:Fatty Acids; D008074:Lipoproteins; D010457:Peptidoglycan; D011509:Proteoglycans; C069988:PplA protein, Legionella pneumophila; C031164:glycerylcysteine; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; D043424:Carboxypeptidase B; D003545:Cysteine