title: Immunochemical assay of rat postheparin plasma triacylglycerol lipases.
abstract: Immunochemical methods for selective measurement of lipoprotein lipase and hepatic lipase activities in rat postheparin plasma are described and validated. Lipoprotein lipase was measured using a substrate containing 10% serum and 0.1 M NaCl after inactivation of hepatic lipase with a specific antiserum. Hepatic lipase was measured at 1.0 M NaCl with a serum-free substrate. The heparin dose-response curve indicated maximum relase of both activities at a heparin dose of 500 IU/kg. The lipase activities in rat postheparin plasma were 3 to 4-fold higher than those in human postheparin plasma. The LPL activity in female rats was significantly higher than in males whereas there was no sex difference for hapatic lipase.
mesh_terms: D000818:Animals; D005260:Female; D006493:Heparin / Q000494:pharmacology; D007158:Immunologic Techniques / Q000379:methods*; D008049:Lipase / Q000032:analysis* / Q000097:blood / Q000276:immunology; D008071:Lipoprotein Lipase / Q000097:blood* / Q000276:immunology; D008099:Liver / Q000201:enzymology; D008297:Male; D051381:Rats
chemical_list: D006493:Heparin; D008049:Lipase; D008071:Lipoprotein Lipase