Estradiol-induced biochemical changes in human neoplastic cells: estradiol-mediated protease.
One human skin fibroblast (HSF), three normal mammary epithelial (HNMEC), four mammary carcinoma (HMCC) and three malignant melanoma (HMMC) cell lines were examined for responsiveness to 17 alpha- and 17 beta-estradiol. Responsiveness was monitored by changes in 3H-thymidine uptake (DNA synthesis), 3H-leucine uptake (protein synthesis), extranuclear protease activities and resistance to complement-dependent serum cytotoxicity (CDSC). 17 alpha-estradiol had no effect on any of the examined cell lines. 17 beta-estradiol had no effect on the extranuclear protease activities, or resistance to CDSC, but it increased DNA and protein synthesis in HSF and HNMEC cell lines. 17 beta-estradiol increased sensitivity to CDSC cellular lysis, DNA and protein synthesis. It induced qualitative and quantitative changes in the extranuclear protease activities suggesting an estradiol-dependent enzyme. Extracts of estradiol-treated human mammary carcinoma and human malignant melanoma cells yielded concentrated protease activities. Fractionation of the extracts gave enzymically active protein which differed in their catalytic properties.
D001943:Breast Neoplasms / Q000201:enzymology; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007700:Kinetics; D008545:Melanoma / Q000201:enzymology; D009363:Neoplasm Proteins / Q000096:biosynthesis; D010447:Peptide Hydrolases / Q000378:metabolism*; D014176:Protein Biosynthesis; D012867:Skin / Q000201:enzymology; D013379:Substrate Specificity
D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D004958:Estradiol; D004247:DNA; D010447:Peptide Hydrolases