Detection of T and B cell antigens hybridoma monoclonal antibodies: a biotin-avidin-horseradish peroxidase method.
Acetone-fixed frozen sections of reactive lymph node, spleen, three B cell lymphomas, and three T cell lymphomas were studied for the presence of Ia-like antigens and two T cell antigens. Detection of the binding of the hybridoma monoclonal antibodies to these antigens took advantage of the biotin-avidin interaction. The detection method employed a biotin conjugate of goat anti-mouse antibody and an avidin conjugate of horseradish peroxidase. B cell lymphoma cells stained for Ia. The cell lymphomas were shown to be heterogeneous for the expression of the two T cell antigens. these three antigens were generally not detectable after 10% formalin fixation of B-5 fixation. Detection of these antigens and the method employed should prove useful in the immunologic categorization of human lymphomas.
D000818:Animals; D000906:Antibodies; D000954:Antigens, Surface; D001402:B-Lymphocytes / Q000276:immunology*; D002999:Clone Cells; D006041:Goats / Q000276:immunology; D000949:Histocompatibility Antigens Class II; D006651:Histocytochemistry; D006801:Humans; D006822:Hybrid Cells; D007124:Immunoenzyme Techniques; D008223:Lymphoma / Q000145:classification / Q000276:immunology*; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*
D000906:Antibodies; D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II