Protein A-peroxidase conjugates for two-stage immunoenzyme staining of intracellular antigens in paraffin-embedded tissues.
Staphylococcal protein A conjugated to horseradish peroxidase was employed in an indirect immuno-staining technique to identify intracellular antigens in paraffin-embedded tissues. The sections were incubated with specific antisera and the antigen-IgG complexes demonstrated with protein A-peroxidase conjugate. Immunoglobulins, lysozyme and insulin were satisfactorily detected by this technique. A comparison of this method with the PAP, "labelled antigen" and peroxidase-labelled antibody sandwich techniques was made.
D000941:Antigens / Q000032:analysis*; D001853:Bone Marrow / Q000276:immunology; D006651:Histocytochemistry / Q000379:methods*; D006689:Hodgkin Disease / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000276:immunology; D007328:Insulin / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D009101:Multiple Myeloma / Q000276:immunology; D009113:Muramidase / Q000276:immunology; D010179:Pancreas / Q000276:immunology; D010190:Pancreatic Neoplasms / Q000276:immunology; D013205:Staphylococcal Protein A
D000941:Antigens; D007136:Immunoglobulins; D007328:Insulin; D013205:Staphylococcal Protein A; D009113:Muramidase