Highly specific solid phase immunoextraction of TSH using anti-TSH antibodies purified by affinity chromatography.
Highly specific anti-TSH antibodies were purified from a crude antiserum preparation by affinity chromatography using TSH-sepharose column as the adsorbant. This procedure completely eliminated the original LH binding activities. The purified anti-TSH antibodies were then coupled to CNBr activated sepharose 4 B and used in separating TSH from rat pituitaries by batch procedure. This method is demonstrated to be highly specific for purifying labelled TSH from pituitaries previously incubated in the presence of [14C]tyrosine. The effect of TRH on TSH biosynthesis is presented as a biological application of this procedure.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods; D007163:Immunosorbent Techniques; D010902:Pituitary Gland / Q000032:analysis; D051381:Rats; D013972:Thyrotropin / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification*; D014443:Tyrosine / Q000378:metabolism
D000906:Antibodies; D014443:Tyrosine; D013972:Thyrotropin