Regulation of antibody response to SRBC. I. Influence of corpuscular and soluble form of erythrocyte antigen and antigen dose on antibody response in A/J and C57BL/10ScSn mice.
Mice of the inbred strain C57BL/10ScSn compared to the A/J strain produce ten times fewer IgG plaque-forming cells and serum IgG antibodies after primary and secondary SRBC immunization. Even after a repeated immunization with SRBC, the titres of IgG anti SRBC antibodies in serum do not reach the levels found in A/J mice. On the other hand, IgM anti SRBC antibodies are higher in C57BL/10ScSn mice and remain high, even after repeated immunization. Doses of antigen ranging from 1 X 10(7) to 1 X 10(10) SRBC per mouse i.p. did not change significantly the response of C57BL/10ScSn mice. Only the highest dose slightly increased IgG anti SRBC antibody production. The use of a soluble erythrocyte antigen with similar antigenic properties as the original corpuscular form did not change the nature of IgM and IgG anti SRBC antibody response.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D006462:Hemolytic Plaque Technique; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D012756:Sheep / Q000276:immunology; D012995:Solubility
D000941:Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M