Expression of common acute lymphoblastic leukaemia antigen and terminal deoxynucleotidyl transferase in normal mononuclear blood cells during diffusion chamber culture.
Peripheral mononuclear blood cells of 2 healthy individuals were cultured over a 13 d period in diffusion chambers. A high percentage of cALL antigen positive cells and later on TdT containing cells appeared during culture. The cALL+ cells could be clearly distinguished into 2 groups: a population with weak and diffuse fluorescence and a small number of ring-like brightly dyed cells. The first group develops into TdT+ cells and therefore resembles immature T-cells. The interpretation of the ring-like stained cells is still preliminary, but it cannot be excluded, that these cells represent a cell population with more than lymphatic differentiation capacity.
D000951:Antigens, Neoplasm / Q000032:analysis*; D002454:Cell Differentiation; D002478:Cells, Cultured; D004253:DNA Nucleotidylexotransferase / Q000032:analysis*; D004254:DNA Nucleotidyltransferases / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007945:Leukemia, Lymphoid / Q000201:enzymology / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*
D000951:Antigens, Neoplasm; D004254:DNA Nucleotidyltransferases; D004253:DNA Nucleotidylexotransferase