Identification of a subpopulation of chicken B lymphocytes by the lectin from Lotus tetragonolobus.
Lectin-reactive chicken lymphoid cells were detected by agglutination. The lectin from Lotus tetragonolobus agglutinated cells from bursa and spleen and did not agglutinate cells from thymus or peripheral blood of 28-day-old chickens. The percentages of Lotus tetragonolobus reactive cells were also measured by assays of lectin-induced rosette formation, binding of lectin-labelled latex beads, and binding of rhodamine-labelled lectin. The distribution of lectin reactive cells varied with the age of the chicken. The lectin appears to identify a unique subpopulation of chicken B lymphocytes.
D000818:Animals; D001402:B-Lymphocytes / Q000145:classification* / Q000276:immunology; D001773:Blood Cells / Q000032:analysis; D002060:Bursa of Fabricius / Q000032:analysis; D002645:Chickens; D005455:Fluorescent Antibody Technique; D007841:Latex Fixation Tests; D037102:Lectins / Q000494:pharmacology*; D012397:Rosette Formation; D013154:Spleen / Q000032:analysis; D013950:Thymus Gland / Q000032:analysis
D037102:Lectins