Magnetic solid-phase enzyme immunoassay for the detection of anti-glomerular basement membrane antibodies.
A new enzyme immunoassay has been developed for the demonstration of antiglomerular basement membrane antibodies. Magnetically responsive polyacrylamide-agarose beads (Magnogel) activated with glutaraldehyde were used to bind sonicated insoluble rat glomerular basement membranes. Both the collagenous and the non-collagenous moieties were demonstrated to be fixed on the beads. Sera from brown Norway rats with anti-glomerular basement membrane antibodies induced by HgCl2 injections were incubated with the beads. After washing, the fixed rat IgG were revealed using alkaline phosphatase labelled Fab fragments from anti-rat IgG sheep, IgGs. Comparison with a radioimmunoassay showed that results were reliable. This enzyme immunoassay has several advantages which may render this assay of considerable clinical usefulness.
D000180:Acrylic Resins / Q000378:metabolism; D000596:Amino Acids; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000941:Antigens; D001485:Basement Membrane / Q000032:analysis / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007678:Kidney Glomerulus / Q000032:analysis / Q000276:immunology*; D008280:Magnetics; D009115:Muridae; D011863:Radioimmunoassay; D051381:Rats; D011914:Rats, Inbred BN; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000596:Amino Acids; D000906:Antibodies; D000941:Antigens; D000099166:Polyacrylamides