title: Reactivation of yeast glucose-6-phosphate dehydrogenase denaturated by saturated fatty acids.
abstract: Activity of yeast glucose-6-phosphate dehydrogenase, inactivated by treatment with saturated fatty acids, can be partially restored by incubation in a medium of suitable ionic composition. The effectiveness of ions in the reactivation process is inversely related to their 'chaotropic' properties. Time-dependence of reactivation extent suggests a 2-step mechanism of enzyme inactivation and the existence of an intermediate form that aggregates through a 2nd-order reaction, producing irreversibly inactive enzyme.
mesh_terms: D004793:Enzyme Reactivators; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D019814:Myristic Acid; D009227:Myristic Acids / Q000494:pharmacology*; D009994:Osmolar Concentration; D011489:Protein Denaturation; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D004793:Enzyme Reactivators; D009227:Myristic Acids; D019814:Myristic Acid; D005954:Glucosephosphate Dehydrogenase