Expression of Treponema pallidum antigens in Escherichia coli K-12.
A colony bank of recombinant plasmids harboring Treponema pallidum DNA inserts has been established in Escherichia coli K-12. By using an in situ immunoassay, we identified four E. coli clones that expressed T. pallidum antigens. Thus, recombinant DNA technology may provide powerful new tools for studying the pathogenesis of T. pallidum infection.
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D005798:Genes, Bacterial; D010957:Plasmids; D014170:Transformation, Genetic; D014210:Treponema pallidum / Q000276:immunology*
D000942:Antigens, Bacterial; D004269:DNA, Bacterial