Lymphoscintigraphy by sc injection of 67Ga-citrate.
Lymphoscintigraphy with SC injection of 67Ga-citrate was carried out on patients with lymph node metastasis and malignant lymphoma. The dose given at each injection site was about 200 muCi and scintigraphy was started about 5 min after injection. Metastatic lymph nodes and malignant lymphoma were successfully imaged. This positive delineation corresponded well to cold lesions detected by lymphoscintigraphy with 99mTc-rhenium colloid in metastatic lymph nodes. Hotter lesions in malignant lymphoma were scanned positively with 99mTc-rhenium colloid by this method. In in vitro studies, incorporation of 67Ga-citrate in HeLa S3 cells was about 4 times that in macrophages, when the incubation time was 1 h, while colloidal uptake by macrophages was 13 times that by HeLa S3 cells. The binding of 67Ga to transferrin in lymph and pinocytosis are suggested mechanisms of localization. A combination of colloid- and sc 67Ga-lymphoscintigraphy would be of value in the diagnosis of lymph node disease.
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000000981:diagnostic imaging; D002295:Carcinoma, Transitional Cell / Q000000981:diagnostic imaging; D005260:Female; D005710:Gallium Radioisotopes / Q000008:administration & dosage / Q000378:metabolism; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007279:Injections, Subcutaneous; D008207:Lymphatic Metastasis / Q000000981:diagnostic imaging*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000000981:diagnostic imaging*; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000000981:diagnostic imaging; D011877:Radionuclide Imaging; D012211:Rhenium; D013667:Technetium; D017556:Technetium Compounds; D001749:Urinary Bladder Neoplasms / Q000000981:diagnostic imaging
D005710:Gallium Radioisotopes; D017556:Technetium Compounds; C030576:technetium Tc 99m rhenium colloid; D012211:Rhenium; D013667:Technetium