The role of histones as nuclear autoantigens in drug-related lupus erythematosus.
Patients with drug-related lupus erythematosus produce antibodies to nuclear histones which can be detected by a three-step indirect immunofluorescence technique. Procainamide-related antinuclear antibodies were detected by this technique, but hydralazine-related antinuclear antibodies were not. Certain evidence suggests that antibodies induced by the two drugs are reactive with different subclasses of histones. Hydralazine was shown to interact with a soluble DNA-histone complex, and the resulting interaction rendered the histone moiety resistant to trypsin digestion. This mechanism may help to maintain DNA-histone complexes in a potentially immunogenic form and result in the production of autoantibodies.
D000818:Animals; D000974:Antibodies, Antinuclear; D000917:Antibody Formation; D000918:Antibody Specificity; D000941:Antigens; D001324:Autoantigens; D002467:Cell Nucleus / Q000276:immunology; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006657:Histones / Q000276:immunology*; D006801:Humans; D006830:Hydralazine; D007668:Kidney / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D051379:Mice; D011342:Procainamide / Q000009:adverse effects
D000974:Antibodies, Antinuclear; D000941:Antigens; D001324:Autoantigens; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D006830:Hydralazine; D011342:Procainamide