Detection of plant virus by using purified IgG in ELISA.
An optimized ELISA system for the detection of soybean mosaic virus was developed. Purified anti-soybean mosaic virus IgG, a secondary ovalbumin coat, and a clearly defined optimal concentration of enzyme-conjugated anti-soybean mosaic virus IgG are used. The test detects purified virus, or purified virus added to leaf and seed extracts, at a concentration of 2.5 ng/ml. Various buffers significantly affect detection sensitivity.
D000914:Antibodies, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G; D008828:Microbiological Techniques; D009029:Mosaic Viruses / Q000276:immunology / Q000302:isolation & purification*; D013025:Glycine max
D000914:Antibodies, Viral; D007074:Immunoglobulin G