Fresh and cultured corneal grafts compared by post-operative thickness and endothelial cell density.
Thirty-nine corneas were removed within 6 h post mortem and stored in a moist chamber at 4 degrees C before grafting. The mean donor age was 33 years and the average time between death and grafting was 11 h. Thirty cadaver corneas were selected after trypane blue staining and cultured at 31 degrees C for 24 h before grafting. The mean donor age was 61 years and the mean time between death and culture was 18 h. During the first 10 postoperative days fresh grafts were thinner than cultured grafts. One year after the transplantation the two groups did not differ significantly in regard to the clinical result, corneal thickness, or endothelial cell loss. This indicates that corneas from old donors with extended post mortem time can be used for transplantation after individual evaluation and corneal culture.
D000328:Adult; D003315:Cornea / Q000166:cytology; D003316:Corneal Diseases / Q000601:surgery*; D016039:Corneal Transplantation; D004727:Endothelium / Q000166:cytology; D005500:Follow-Up Studies; D006801:Humans; D008875:Middle Aged; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods