[Comparative immunofluorescent-histological investigations on 7 nematode species in respect to its antigenic properties for differential diagnosis of nematode infections (author's transl)].
Five nematode species of the Onchocercidae family and one from the Strongylidae and Trichuridae family were tested as antigen in the immunofluorescence test (IIFT) against reference sera from six nematode infections. The localisation of the cytosomal antigen antibody-reaction in the IIFT which was described on D. Viteae, was found to be the same for B. malayi, B. pahangi, L. carinii, O. volvulus and A. caninum. This test, just as egg membrane crude preparations of 5 nematodes from the Onchocercidae family, could not differentiate between W. bancrofti, onchocerciasis, loiasis and D. perstans sera. The cuticular fluorescence of five Onchocercidae species, which was observed only in frozen sections, appeared only with W. bancrofti-filariasis and onchocerciasis sera. T. spiralis larvae antigen exhibited only cuticular fluorescence in the frozen section, but in the case, however, against all reference sera. Trichinellosis patients' serum reacted on the other hand only with T. spiralis antigen. Uterine microfilaria from B. malayi, L. carinii, O. volvulus and D. viteae could differenciate species specifically between reference sera.
D000328:Adult; D000906:Antibodies / Q000276:immunology; D000937:Antigen-Antibody Reactions; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D009349:Nematode Infections / Q000276:immunology*; D009854:Onchocerca / Q000276:immunology; D013320:Strongyloidea / Q000276:immunology; D014259:Trichuroidea / Q000276:immunology
D000906:Antibodies
Vergleichende Immunfluoreszenz-histologische Untersuchungen an 7 Nematoden-Spezies in bezug auf ihre Antigeneigenschaften zur Differenzierung von Nematodeninfektionen.