Analysis of mutagenic activity in cigarette smokers' urine by high performance liquid chromatography.
Mutagenic activity in smokers' urine which had been concentrated by XAD-2 resin can be separated from approximately 90% of the non-mutagenic material by CH2Cl2 extraction. This extract appears to be stable for 3 months at -20 degrees C. High performance liquid chromatography analysis of the CH2Cl2 extract showed multiple mutagenic non-polar fractions which were better activated by rat liver homogenates prepared from 3-methylcholanthrene treated rats than from rats treated with phenobarbital. Mutagenic activity in smokers' urine was extractable by acid, but not by base. The mutagens in smokers' urine appear to be a complex mixture of relatively non-polar chemicals.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis*; D051381:Rats; D012486:Salmonella typhimurium / Q000235:genetics; D012907:Smoking; D014556:Urine / Q000032:analysis*
D009153:Mutagens