title: Microtubule complexes correlated with growth rate and water proton relaxation times in human breast cancer cells.
abstract: Ten established human breast cancer cell lines display patterns of microtubule organization which are characterized by growth rate of the cell populations and the freedom of mobility of cellular water molecules measured by nuclear magnetic resonance spectroscopy. Cell lines with population-doubling times of 1 to 2 days demonstrate rapid mobility of water molecules by proton spin-lattice and spin-spin relaxation times (T1 greater than 750 msec, T2 greater than 120 msec) and have diffuse patterns of tubulin immunofluorescent antibody staining. Moderately fast dividing cells (population-doubling times of 3 to 7 days) have T1 values of 600 to 750 msec and show approximately 50% organized complexes of polymerized microtubules in the cytoplasm. Slow-growing cell lines demonstrate more restricted mobility of water molecules (T1 values of 500 to 600 msec) and contain abundant networks of polymerized microtubules. The three-way correlation of the physical parameter of water proton relaxation times, the structural parameter of microtubule organization, and the physiological parameter of growth suggest a close interaction of water molecules with the cytoplasmic macromolecular network in the performance of physiological function.
mesh_terms: D001834:Body Water / Q000032:analysis*; D001943:Breast Neoplasms / Q000473:pathology / Q000503:physiopathology*; D002455:Cell Division; D002460:Cell Line; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008870:Microtubules / Q000502:physiology* / Q000648:ultrastructure; D014404:Tubulin / Q000032:analysis*
chemical_list: D014404:Tubulin