Histochemical demonstration of terminal deoxynucleotidyl transferase in leukemia.
White blood cells from 22 patients with leukemia and lymphoma were studied for the presence of terminal deoxynucleotidyl transferase with a peroxidase-antiperoxidase method. The enzyme was detected in leukemic cells of 5 patients with acute lymphoblastic leukemia and 1 patient with chronic myelogenous leukemia, whereas 16 patients with different forms of leukemia or lymphoma were negative for this enzyme. Comparative studies using a biochemical and an indirect immunofluorescence assay revealed complete concordance between these three methods.
D000818:Animals; D004253:DNA Nucleotidylexotransferase / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006041:Goats; D006801:Humans; D007124:Immunoenzyme Techniques; D007945:Leukemia, Lymphoid / Q000201:enzymology*; D007951:Leukemia, Myeloid / Q000201:enzymology*; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology*; D008223:Lymphoma / Q000201:enzymology*; D011817:Rabbits
D004253:DNA Nucleotidylexotransferase