title: Disturbed intrarenal distribution of gentamicin in experimental pyelonephritis due to Escherichia coli.
abstract: The intracortical, medullary, and papillary distribution of gentamicin was studied in normal and pyelonephritic rats. The animals were evaluated from 1 hr to 365 days after the end of therapy with either a single dose or two daily injections given every 12 hr for seven days. The serum levels of gentamicin at 1 hr were significantly (P less than 0.001) higher in the pyelonephritic rats than in the normal rats after one dose (26 vs. 12 microgram/ml) and 14 doses (25.7 vs. 8.8 microgram/ml). Peak concentrations or gentamicin in all parts of infected kidneys were significantly (P less than 0.001) higher than in normal kidneys. Gentamicin was still detectable at levels of 1.2 microgram/g in the cortex of one pyelonephritic animal one year after the end of therapy, when the levels of both serum creatinine (1.1 mg/100 ml) and blood urea nitrogen (30 mg/100 ml) were much higher than at seven days after the end of therapy (0.5 and 19 mg/100 ml, respectively).
mesh_terms: D000818:Animals; D004927:Escherichia coli Infections / Q000378:metabolism*; D005260:Female; D005839:Gentamicins / Q000008:administration & dosage / Q000378:metabolism* / Q000652:urine; D007672:Kidney Cortex / Q000378:metabolism*; D007679:Kidney Medulla / Q000378:metabolism*; D007700:Kinetics; D011704:Pyelonephritis / Q000378:metabolism*; D051381:Rats; D014018:Tissue Distribution
chemical_list: D005839:Gentamicins