[Cloning of the flagellate Crithidia oncopelti on a solid nutrient medium of indeterminant composition not containing hemin].
On a simple solid medium of indefinite content without hemin, colonies of Crithidia oncopelti were received. The clonal nature of them was proved. The survival in some experiments varied from 10 to 70%. Young colonies, 6 days after inoculation, were 0.3- 2.0 mm in diameter. C. oncopelti differed from Herpetomonas megaseliae and Crithidia harmosa in the morphology of colonies. To further genetical study of C. oncopelti, a possibility of cloning the object on a simple solid medium is an important prerequisite.
D002999:Clone Cells / Q000166:cytology; D003421:Crithidia / Q000166:cytology*; D003470:Culture Media / Q000378:metabolism; D003584:Cytological Techniques; D006418:Heme / Q000031:analogs & derivatives*; D006427:Hemin / Q000378:metabolism*; D013997:Time Factors
D003470:Culture Media; D006418:Heme; D006427:Hemin
Klonirovanie zhgutikonostsa Crithidia oncopelti na plotnoĭ pitatel'noĭ srede neopredelnnogo sostava, na soderzhashcheĭ gemin.