Detection of terminal transferase in paraffin sections with the immunoperoxidase technique.
Terminal deoxynucleotidyl transferase (TdT) is an early T-cell differentiation marker in a number of species, including man. We have demonstrated TdT in the nuclei of cortical thymocytes in paraffin-embedded sections of calf, rat, and human thymus by indirect immunoperoxidase techniques. In addition, these techniques have been used to verify and extend enzyme assay results by detecting TdT in blast cells from 10 patients with convoluted T-cell lymphoma/leukemia, 1 patient with acute granulocytic leukemia, 1 patient with chronic granulocytic leukemia in blast crisis, and 1 patient with non-T non-B acute lymphocytic leukemia.
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000201:enzymology; D004254:DNA Nucleotidyltransferases / Q000032:analysis*; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D007938:Leukemia / Q000201:enzymology; D008198:Lymph Nodes / Q000201:enzymology; D008213:Lymphocyte Activation; D008297:Male; D010232:Paraffin; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000201:enzymology; D013601:T-Lymphocytes / Q000201:enzymology*; D013950:Thymus Gland / Q000201:enzymology
D007106:Immune Sera; D010232:Paraffin; D004254:DNA Nucleotidyltransferases