Induction of various androgen-dependent esteroproteases (trypsin-like and chymotrypsin-like enzymes) by tri-iodo-L-thyronine in the submandibular glands of female mice and mice with testicular feminization.
Trypsin-like and chymotrypsin-like esteroprotease isozymes of the mouse submandibular gland were separated by isoelectric focusing. In normal female mice the following pI-isozyme activities were found; pI-4.6, -5.6 (shoulder), -5.8, -7.1, and -9.9, hydrolytic activities for benzoylarginine ethylester (BAEE) (trypsin-like enzymes), and pI-4.7 and -10.3 hydrolytic activities for acetyltyrosine ethylester (ATEE) (chymotrypsin-like enzymes). In mice with testicular feminization (Tfm mice), only pI4.6 hydrolytic activity for BAEE was found; no ATEE hydrolytic activity was detected. In normal female mice, both 5 alpha-dihydrotestosterone (5 alpha-DHT) and tri-iodo-L-thyronine (T3) significantly increased all these isozymes except the pI-4.6 hydrolytic activity for BAEE. In Tfm mice, T3 also increased all these isozymes except the pI-4.6 hydrolytic activity for BAEE, but 5 alpha-DHT had no effect on any enzymes. These results suggest that the pI-4.6 hydrolytic activity for BAEE is non-inducible by the two hormones. Androgen does not seem to be involved in the inductions of these esteroproteases by T3.
D013734:Androgen-Insensitivity Syndrome; D000728:Androgens / Q000502:physiology; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism; D002918:Chymotrypsin / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism*; D004790:Enzyme Induction; D005260:Female; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D051379:Mice; D013363:Submandibular Gland / Q000201:enzymology*; D014284:Triiodothyronine / Q000494:pharmacology*; D014357:Trypsin / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D000728:Androgens; D007527:Isoenzymes; D014284:Triiodothyronine; D014443:Tyrosine; C007651:ethyl N-alpha-acetyl-tyrosinate; D001120:Arginine; C009727:benzoylarginine ethyl ester; D010450:Endopeptidases; D002918:Chymotrypsin; D014357:Trypsin