[Modification of RNA polymerase from Escherichia coli by fluoresceinbimercuriacetate].
RNA polymerase was modified by fluoresceinbimercuriacetate (FMA) at SH-groups. One molecule of the enzyme (M. W. 500,000) was shown to bind two molecules of FMA. The first molecule reacts with the most reactive SH-groups causing 50%-inhibition of RNA synthesis. The binding of the second one results in 100%-inactivation of the enzyme. Considerable changes in spectral properties of the dye are observed when it interacts with RNA polymerase.
D001665:Binding Sites; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors*; D004926:Escherichia coli / Q000201:enzymology*; D005452:Fluoresceins / Q000494:pharmacology*; D007700:Kinetics; D008970:Molecular Weight; D011485:Protein Binding
D005452:Fluoresceins; D012321:DNA-Directed RNA Polymerases
Modifikatsiia RNK-polimerazy Escherichia coli fluorestseinbimerkuratsetatom.