Detection of cell-surface antigens in murine tumors by a protein A immunofluorescence test.
The detection of antibodies to H-2 and species cell-surface antigens, on suspensions of leukemia and MC-induced sarcomas by an assay using fluorescein-labelled protein A from Staphylococcus aureus is described. The presence of 'foreign' H-2 allospecificities on MC-induced sarcoma cells were detected. Antibodies to leukemia and MC sarcoma cells showed a high degree of cross-reactivity in their reaction with different MC-induced sarcomas and with leukemia cells.
D000818:Animals; D000954:Antigens, Surface; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006183:H-2 Antigens; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G; D007942:Leukemia, Experimental / Q000276:immunology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell; D012513:Sarcoma, Experimental / Q000276:immunology*; D013205:Staphylococcal Protein A
D000954:Antigens, Surface; D006183:H-2 Antigens; D007106:Immune Sera; D007074:Immunoglobulin G; D011947:Receptors, Antigen, B-Cell; D013205:Staphylococcal Protein A