A new assay for leukocyte chemotaxis using cell retrieval, electronic particle counting and flow cytometry.
A new micropore membrane assay for leukocyte migration has been devised. It permits the complete retrieval in monodisperse suspension of functionally intact cells that have traversed the membrane, thus allowing the application of precise, automated techniques, including flow cytometry and electronic particle counting. Hemocytometers may also be used. Direct comparison with 2 different conventional membrane methods showed that the new method performed superiorly. It was also much more economical with regard to time and labor. This technique permitted detection of functional differences between leukocytes isolated from blood in different ways. Data on the duration of concentration gradients in chemotaxis chambers are also presented.
D000328:Adult; D002630:Chemotactic Factors / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte; D004305:Dose-Response Relationship, Drug; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016209:Interleukin-8; D007958:Leukocyte Count / Q000379:methods; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology; D013997:Time Factors; D015054:Zymosan / Q000494:pharmacology
D002630:Chemotactic Factors; D016209:Interleukin-8; D008567:Membranes, Artificial; D015054:Zymosan