Maltose and maltodextrin transport in Escherichia coli.
Escherichia coli K12 strains producing reduced amounts of LamB protein (one tenth the wild type amount) grow normally on dextrins but transport maltose at a concentration of 1 microM at about one tenth the normal rate. Dex--lamB missense mutants were found as derivatives of these strains. These Dex- mutants had a structurally altered LamB protein in the outer membrane, impaired in its interaction with phages lambda and K10, inefficient in maltose transport at low concentration (10 microM and below). The Dex- mutants still produced one tenth the wild type amount of the LamB protein as the parental Dex+ strains. Analysis of Dex+ revertants showed that the phenotype reverts to Dex+ when the altered LamB protein is made in wild type amounts. Even though they were Dex+, these revertants were still drastically altered in maltose transport at low concentration.
D018528:ATP-Binding Cassette Transporters; D001425:Bacterial Outer Membrane Proteins; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003912:Dextrins / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005947:Glucose / Q000378:metabolism; D008320:Maltose / Q000031:analogs & derivatives / Q000378:metabolism*; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D009154:Mutation; D011134:Polysaccharides / Q000378:metabolism*; D018272:Porins; D011991:Receptors, Virus / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D003912:Dextrins; D029968:Escherichia coli Proteins; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D011134:Polysaccharides; D018272:Porins; D011991:Receptors, Virus; C016532:maltoporins; C461599:maltose transport system, E coli; D008320:Maltose; C008315:maltodextrin; D005947:Glucose