Insulin modulation of antibody-dependent cytotoxicity and the detection of antireceptor antibodies.
Insulin and insulin receptors may play a role in the modulation of cytotoxic effector cell function. We have investigated the effects of insulin on ADCC. At low effector-to-target cell ratios, the addition of insulin resulted in a dose-dependent reduction of erythrocyte target cell lysis but not lysis of tumor target cells. In a patient with insulin resistance, total lipodystrophy, and a demonstrable reduction in specific insulin binding to circulating monocytes, ADCC for erythrocyte target cells was unaffected by the addition of insulin. In addition, incubation of normal effector cells in patient's serum similarly abrogated the effect of insulin. The failure of insulin to inhibit ADCC for erythrocyte target cells may serve as a reliable and simple assay for the demonstration of altered insulin receptors or for the detection of anti-insulin receptor antibodies.
D000328:Adult; D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects*; D001323:Autoantibodies; D002645:Chickens; D004351:Drug Resistance; D005260:Female; D006801:Humans; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology*; D008060:Lipodystrophy / Q000276:immunology; D008545:Melanoma / Q000276:immunology; D009000:Monocytes / Q000378:metabolism; D011972:Receptor, Insulin / Q000276:immunology*
D001323:Autoantibodies; D007328:Insulin; D011972:Receptor, Insulin