Clinical utility of leukemia cell terminal transferase measurements.
Interest in the DNA-synthetic enzyme terminal deoxynucleotidyl transferase (TdT) has developed from two sets of observations: first, in normal animals, it occurs only in immature thymic lymphocytes and in a subpopulation of bone marrow lymphocytes; second, it is present in the blast cells of almost all patients with acute lymphoblastic leukemia. A prospective trial to evaluate blast cell TdT as a predictor of responsiveness to vincristine and prednisone in 30 Philadelphia chromosome-positive patients with blastic chronic myelogenous leukemia was undertaken. Eleven of 16 TdT-positive patients responded, whereas only one of 14 TdT-negative patients showed improvement. Among TdT-positive patients under the age of 50 years, the response rate was 78%. Enzyme-negative patients under the age of 50 had an 11% response rate. Blast cell morphology (i.e., lymphoblastic versus myeloblastic) had no significant correlation with either responsiveness or TdT activity. These results suggest that blast cell TdT activity may identify leukemic patients who are likely to respond to vincristine and prednisone irrespective of their conventional classification.
D000293:Adolescent; D000328:Adult; D000368:Aged; D002454:Cell Differentiation; D002648:Child; D002675:Child, Preschool; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D006410:Hematopoiesis; D006801:Humans; D007938:Leukemia / Q000145:classification / Q000201:enzymology*; D007945:Leukemia, Lymphoid / Q000201:enzymology* / Q000628:therapy; D007951:Leukemia, Myeloid / Q000188:drug therapy / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology; D008875:Middle Aged; D011241:Prednisone / Q000627:therapeutic use; D013601:T-Lymphocytes / Q000201:enzymology; D014750:Vincristine / Q000627:therapeutic use
D014750:Vincristine; D004254:DNA Nucleotidyltransferases; D004253:DNA Nucleotidylexotransferase; D011241:Prednisone