title: Immuno-electron-microscopic identification of O-antigen-bearing oligodendroglial cells in vitro.
abstract: Monoclonal antibodies to cell-surface antigens of oligodendrocytes (Sommer and Schachner 1980; Schachner et al. 1980) were used to identify this cell type by immuno-electron microscopy in monolayer cultures of fetal and early postnatal mouse cerebellum. The ultrastructural features of antigen-positive cells confirm that they are immature and mature oligodendrocytes, but not neurons, astrocytes or fibroblasts or fibroblast-like cells. Type I oligodendrocytes are the immature ones with a relatively large amount of moderately electron-lucent cytoplasm, clusters of ribosomes and complex networks of rough endoplasmic reticulum. Large numbers of mitochondria and microtubules, but not intermediate-sized filaments are seen in these cells. They comprise more than 90% of all 0-antigen-positive cells. Type II cells comprise only approximately 5% of all 0-antigen-positive cells. They are characterized by a limited amount of electron-dense cytoplasm, which appears more compact and granular than in type I cells. The rough endoplasmic reticulum is distributed evenly throughout the cytoplasm. Microtubules and mitochondria are present, but more difficult to distinguish due to the compactness of the cytoplasm. Type II cells display the more mature ultrastructural features of oligodendrocytes.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology; D007124:Immunoenzyme Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D009457:Neuroglia / Q000166:cytology*; D009836:Oligodendroglia / Q000166:cytology* / Q000276:immunology; D009940:Organoids / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface