[Obtaining a primary mouse hepatocyte culture].
A method of isolating and culturing mouse hepatocytes is described. The mouse liver was subsequently perfused in situ with Ca-free and collagenase containing solutions, mechanically dispersed and purified by centrifugation. The suspension obtained contained up to 10(7) parenchymal cells, whose viability being not less than 80-90%. In the suspension, the mean diameter of hepatocytes was 25 micron. On being plated on plastic Petry dishes in concentrations of 1-5.10(5)/ml and in the presence of serum, hepatocytes were attached to the substrate in 2-3 hours to form cell monolayers. The cultured hepatocytes maintained their viability for 5-7 days.
D000818:Animals; D002478:Cells, Cultured; D003584:Cytological Techniques; D008099:Liver / Q000166:cytology*; D051379:Mice; D010477:Perfusion / Q000379:methods*; D013535:Suspensions
D013535:Suspensions
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