Enhancement of invasiveness of Yersinia enterocolitica and Escherichia coli in HEp-2 cells by centrifugation.
Centrifugation enhanced the infectivity of invasive Escherichia coli and Yersinia enterocolitica for HEp-2 cells. Noninvasive bacteria were not endocytosed after centrifugation. The centrifugation procedure may increase the sensitivity of testing for bacterial invasiveness in cell culture without causing false-positive results.
D000268:Adhesiveness; D001431:Bacteriological Techniques; D002460:Cell Line; D002498:Centrifugation; D004926:Escherichia coli / Q000254:growth & development / Q000472:pathogenicity*; D006801:Humans; D015007:Yersinia / Q000254:growth & development / Q000472:pathogenicity*