[Studies on degradation of cartilage proteoglycan by rheumatoid synovial tissue. Part I: On the degraded products of proteoglycan (author's transl)].
The degradation of MgCl2-extracted proteoglycans (PG) from bovine nasal and articular cartilage was performed by using human rheumatoid synovial tissue extract, and the products were compared with that of being degraded by different commercially available proteases. 2. Degradation of PG by crude synovial extract occurred under the wide range of pH, and the degradation products showed the changes as follows; 1) a decrease of viscosity, 2) a decrease of hydrodynamic size, 3) an increase of electrophoretic mobility, 4) no change of the length of glycosaminoglycan side chains, 5) an increase of chondroitin sulfate relative to keratan sulfate in precipitate with 1% cetylpyridinium chloride (CPC), and 6) an increase of serine content in precipitate with 1% CPC. 3. The average hydrodynamic size of degradation products by crude synovial extract was larger than that of degradation products by pronase or papain. 4. The results indicate that the proteases play the main role on the PG-degrading activity of rheumatoid synovial tissue extract but the glycosidase play no significant role.
D000328:Adult; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D002358:Cartilage, Articular / Q000378:metabolism*; D002417:Cattle; D005260:Female; D006801:Humans; D008875:Middle Aged; D010447:Peptide Hydrolases / Q000494:pharmacology; D011509:Proteoglycans / Q000378:metabolism*; D013583:Synovial Membrane / Q000032:analysis*; D014783:Viscosity
D000596:Amino Acids; D011509:Proteoglycans; D010447:Peptide Hydrolases