High cloning efficiency of human lymphoid cells in agarose without feeder layers.
A protocol has been developed that consistently gives high cloning efficiencies for human lymphoid cell lines (lymphoblastoid and lymphoma) in agarose without the use of feeder layers. This procedure utilizes a cloning medium that contains horse serum, alpha-ketoglutarate or oxalacetate, and high levels of glutamine.
D002460:Cell Line; D002999:Clone Cells; D003470:Culture Media; D003584:Cytological Techniques; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*; D008223:Lymphoma; D012685:Sepharose
D003470:Culture Media; D012685:Sepharose