title: Isolation and characterization of rat plasma fibronectin.
abstract: Rat plasma fibronectin has been isolated and characterized and monospecific antibodies were prepared to it. Two components of fresh rat plasma (in the presence of proteinase inhibitors) bound to a gelatin-Sepharose affinity column. One protein was eluted with 4.0 M-urea and was identified as fibronectin. Another protein was eluted from the gelatin-Sepharose column with 8.0 M-urea and was identified as a 70 000-Mr collagen-binding molecule. This 70 000-Mr fragment was found to be a normal constituent of blood plasma, and its presence did not represent a proteolytic degradation product formed during isolation. The antibodies prepared against rat fibronectin only weakly cross-reacted with plasma fibronectins of chicken, horse and human. These studies shed light on the metabolic interrelationships between fibronectin and other collagen-binding molecules.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000906:Antibodies; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification; D005455:Fluorescent Antibody Technique; D051381:Rats; D013045:Species Specificity
chemical_list: D000596:Amino Acids; D000906:Antibodies; D005353:Fibronectins