title: Study of asialogangliosides on surface membranes of rat bone marrow cells and macrophages.
abstract: The presence of asialogangliosides on membrane surfaces of rat bone marrow cells and macrophages was studied by an immunological method using anti-glycolipid antibodies with high specificity. The antibodies against N-acetylgalactosaminyl(beta 1-4)galactosyl(beta 1-4)glucosylceramide (GA2), galactosyl(beta 1-3)N-acetylgalactosaminyl(beta 1-4)galactosyl(beta 1-4)glucosylceramide (GA1) and fucosyl(alpha 1-2)galactosyl(beta 1-3)N-acetylgalactosaminyl(beta 1-4)galactosyl(beta 1-4)glucosylceramide (Fuco-GA1) were prepared, and used for cytotoxicity testing and indirect staining by the immunofluorescence technique. In the cytotoxicity test, approximately 40-50% of rat intraperitoneal macrophages were killed by each of the anti-glycolipid antisera. This finding was confirmed by the indirect immunofluorescence staining method with the purified anti-GA2, anti-GA1, and also anti-Fuco-GA1 IgG. When a rat was preimmunized with bovine serum albumin, all macrophages induced in ascites fluid were found to possess GA1 by the staining method. Immature granulocytes were stained specifically with the anti-GA2 antibody. Granulocytes, myelocytes, and megakaryocytes were stained with the anti-GA1 antibody. Blastic myelocytes were stained with the anti-Fuco-GA1 IgG, but lymphocytes were not stained with any of these anti-asialoganglioside antibodies. From these observations, free-type cells such as macrophages and bone marrow cells were found to possess asialogangliosides as membrane components.
mesh_terms: D000818:Animals; D000906:Antibodies; D001854:Bone Marrow Cells; D002462:Cell Membrane / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006017:Glycolipids / Q000032:analysis / Q000276:immunology; D006028:Glycosphingolipids / Q000032:analysis*; D008264:Macrophages / Q000032:analysis*; D051381:Rats
chemical_list: D000906:Antibodies; D006017:Glycolipids; D006028:Glycosphingolipids