title: Studies on bonnet monkey cervical mucus. The effect of proteases on mucus glycoproteins of Macaca radiata.
abstract: The influence of proteinases on monkey cervical glycoproteins was investigated to assess their effect on cervical mucus and, thereby, on sperm penetration. The major component of periovulatory cervical mucus, a high molecular weight glycoprotein, was treated with Pronase, trypsin, chymotrypsin, papain, and bovine seminal peptidase, and the enzyme-resistant glycoprotein was purified by gel filtration on Sepharose 2B. A macromolecular component in high yield was recovered containing carbohydrate and protein moieties. Asialoglycoprotein, on treatment with Pronase, trypsin, and bovine seminal peptidase released more than one glycoprotein fragment. The carbohydrate and amino acid components of the native and degraded glycoproteins were similar in composition with variations in proportions. The structure of the carbohydrate-rich, pronase-resistant glycoprotein, further purified on Sepharose 2B, was examined. Sequential Smith degradation and methylation of the degraded glycoprotein fragment established a structure that shows some differences to that of the native glycoprotein. The influence of proteinases on cervical-mucus glycoproteins and a possible mechanism of sperm penetration through Pronase-treated glycoproteins is discussed.
mesh_terms: D000818:Animals; D002417:Cattle; D002582:Cervix Mucus / Q000378:metabolism*; D002918:Chymotrypsin / Q000494:pharmacology; D010450:Endopeptidases / Q000494:pharmacology*; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D008255:Macaca radiata; D008297:Male; D008970:Molecular Weight; D010206:Papain / Q000494:pharmacology; D010447:Peptide Hydrolases / Q000494:pharmacology; D011402:Pronase / Q000494:pharmacology; D012661:Semen / Q000201:enzymology; D013084:Sperm-Ovum Interactions / Q000187:drug effects; D014357:Trypsin / Q000494:pharmacology
chemical_list: D006023:Glycoproteins; D010450:Endopeptidases; D010447:Peptide Hydrolases; D002918:Chymotrypsin; D014357:Trypsin; D010206:Papain; D011402:Pronase