Biological activity of chemically synthesized analogues of lipid A. Demonstration of adjuvant effect in hapten-sensitized liposomal system.
The adjuvant effect of synthetic analogues of lipid A was investigated in mice in vivo. Two of the five synthetic lipid A analogues showed adjuvant activity when they were incorporated into dipalmitoylglycerophosphocholine-cholesterol-dipalmitoylglycerophosphate liposomes sensitized with trinitrophenylaminocaproylglycerophosphoethanolamine. The minimum structure for an adjuvant effect in the liposomal immunogen system was 1-monophosphate of 6-0-(2-deoxy-2-tetradecanoylamino-6-0-tetradecanoyl-D-glucopyranosyl)-2-deoxy-2 -tetradecanoyl-amino-3,4-di-O-tetradecanoyl-D-glucopyranose.
D000276:Adjuvants, Immunologic; D000818:Animals; D000917:Antibody Formation; D004912:Erythrocytes / Q000276:immunology; D006241:Haptens; D006462:Hemolytic Plaque Technique; D008050:Lipid A / Q000031:analogs & derivatives / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D008081:Liposomes; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D012756:Sheep; D013329:Structure-Activity Relationship
D000276:Adjuvants, Immunologic; D006241:Haptens; D008050:Lipid A; D008070:Lipopolysaccharides; D008081:Liposomes