[Influence of embedding media on the fading of FITC-labelled coverslip cultures [author's transl)].
The influence of embedding media on the fading of FITC-labelled coverslip cultures was tested. It was found, that the value of the fading depend on the pH value of the embedding media. In glycerin-PBS pH 8.5 as embedding media and immediate measuring the fluorescence intensity, the decrease of the fluorescence intensity after 300 seconds blue light excitation was 5%.
D000818:Animals; D002021:Buffers; D002460:Cell Line; D003470:Culture Media; D029822:Enterovirus B, Human / Q000254:growth & development; D005455:Fluorescent Antibody Technique; D005990:Glycerol; D006863:Hydrogen-Ion Concentration
D002021:Buffers; D003470:Culture Media; D005990:Glycerol
Einfluss von Einbettungsmitteln auf den Ausbleicheffekt von FITC-markierten Deckglaspräparaten.