Kinetic serum creatinine assays. II. A critical evaluation and review.
We have evaluated the Technicon AutoAnalyzer method, the Beckman Creatinine Analyzer II, DuPont aca, Electro-Nucleonics GEMSAEC, and Instrumentation Laboratory Model 916 kinetic methods for determination of serum creatinine. These methods, which involve the Jaffé reaction, all showed a statistically significant proportional error relative to an ion-exchange pretreatment method for creatinine in 92 serum samples; the GEMSAEC method also showed a bias. The positive interference of several acid metabolites was quantitated in each of the methods. The evidence indicates that bilirubin, although associated with a negative creatinine interference, is not the sole interferent. We determined the magnitude of this interference and studied several methods for minimizing it.
D001322:Autoanalysis; D001663:Bilirubin / Q000378:metabolism; D003404:Creatinine / Q000097:blood*; D006801:Humans; D007651:Keto Acids / Q000378:metabolism; D007700:Kinetics; D013053:Spectrophotometry
D007651:Keto Acids; D003404:Creatinine; D001663:Bilirubin