Peptone-yeast autolysate-fetal bovine serum 10, a simple, inexpensive liquid medium for cultivation of Leishmania spp.
A simple liquid medium for the cultivation of Leishmania parasites is described. Leishmania brasiliensis and Leishmania peruviana cultured in this medium reached cell densities greater than 10(7) promastigotes per ml within 7 days. This medium compares very favorably with the more complex media used to cultivate Leishmania spp. and other hemoflagellates.
D000818:Animals; D002417:Cattle / Q000097:blood; D003470:Culture Media; D005312:Fetal Blood; D007891:Leishmania / Q000254:growth & development*; D008828:Microbiological Techniques; D010461:Peptones / Q000494:pharmacology; D015003:Yeasts
D003470:Culture Media; D010461:Peptones