Immunobiological properties of 1-butanol-extracted cell surface antigens.
Extracts of viable 3-methylcholanthrene-induced murine sarcoma cells (MCA-F and MCA-2A) prepared using single-phase (2.5%) 1-butanol significantly retarded the outgrowth of the homotypic, but not the heterotypic, tumor of syngeneic C3H/HeJ mice. Butanol extracts specifically evoked a delayed hypersensitivity response in tumor-immune syngeneic mice, but not in alloimmune DBA/2J mice. Crude butanol extracts of MCA-F cells did not contain alloantigenic activity, as shown by their inability to block H-2 or Ia-specific antibodies in a complement-dependent cytotoxicity assay. Absorption of these same allospecific reagents with untreated or with butanol-extracted cells indicated that H-2 antigens remain associated with the cell surface during extraction. Thus, butanol appears to release tumor antigens, but not alloantigens, from the cell surface.
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology* / Q000302:isolation & purification; D000440:Butanols; D005260:Female; D005354:Fibrosarcoma / Q000139:chemically induced / Q000276:immunology*; D007158:Immunologic Techniques; D008748:Methylcholanthrene; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D012513:Sarcoma, Experimental / Q000139:chemically induced / Q000276:immunology*
D000951:Antigens, Neoplasm; D000440:Butanols; D008748:Methylcholanthrene