Utilization by Saccharomyces cerevisiae of 5'-methylthioadenosine as a source of both purine and methionine.
Cells of the yeast Saccharomyces cerevisiae are normally impermeable to the purine nucleosides adenosine and 5'-deoxy-5'-methylthioadenosine (MTA), a product of polyamine biosynthesis. cordycepin-sensitive, adenosine-utilizing strains of S. cerevisiae were able to use MTA to fulfill an auxotrophic requirement for purine. Cordycepin-sensitive strains carrying a met5 mutation were also able to use MTA as a source of methionine. These MTA-utilizing strains of S. cerevisiae should be useful for metabolic studies of the fate of MTA.
D000241:Adenosine / Q000031:analogs & derivatives* / Q000378:metabolism; D003839:Deoxyadenosines; D005838:Genotype; D008715:Methionine / Q000378:metabolism*; D009154:Mutation; D011684:Purine Nucleosides / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D013872:Thionucleosides / Q000378:metabolism*
D003839:Deoxyadenosines; D011684:Purine Nucleosides; D013872:Thionucleosides; C008500:5'-methylthioadenosine; D008715:Methionine; D000241:Adenosine