[Use of DNA antibodies for detecting DNA radiation injuries in the bone marrow cell nuclei of irradiated animals].
Impairments in DNA from brain stem cell nuclei of X-ray irradiated animals were studied at early steps after irradiation using antibodies to single-stranded DNA. A possibility was shown to detect by means of an immunochemical technique the irradiation-induced impairments of DNA in brain stem cells. Detection of the impairment was possible within 3 hrs after high doses of the irradiation (206.4-258 mCi/kg); after lower doses - within the first hour of the postirradiation period. The immunochemical procedure might be used for early biological detection of the irradiation impairments as well as for estimation of radio-sensitivity of nuclear cells to the effects of irradiation and radioprotectors.
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology*; D000918:Antibody Specificity; D001853:Bone Marrow / Q000276:immunology / Q000528:radiation effects*; D002467:Cell Nucleus / Q000276:immunology / Q000528:radiation effects*; D004247:DNA / Q000276:immunology* / Q000528:radiation effects; D004277:DNA, Single-Stranded / Q000276:immunology; D005455:Fluorescent Antibody Technique; D007114:Immunization; D008297:Male; D011817:Rabbits; D011833:Radiation Injuries, Experimental / Q000175:diagnosis* / Q000276:immunology; D051381:Rats; D013997:Time Factors
D000906:Antibodies; D004277:DNA, Single-Stranded; D004247:DNA
Primenenie antitel k DNK dlia vyiavleniia luchevykh povrezhdeniĭ DNK v iadrakh kletok kostnogo mozga obluchennykh zhivotnykh.