Demonstration of sister chromatid differentiation in human amniotic fluid cells after partial synchronization of BrdU or dT surplus.
Protocols are compared demonstrating sister chromatid differentiation (SCD) in human amniotic fluid (AF) cells with and without partial synchronization. Partial synchronization both with an excess of 5-bromodeoxyuridine (BrdU) and an excess of thymidine leads to an increase of metaphases with SCD. Compared with unsynchronized cells, the rate of sister chromatid exchanges (SCE) is not increased. Studies on the late replicating X chromosome of female cells showed that the addition of mitomycin C (MMC) after releasing the thymidine block preferentially induces SCEs in late replicating regions. The partial synchronization with thymidine surplus provided a good basis for SCE experiments with AF cells and facilitates the prenatal diagnosis of diseases characterized by changes in the SCE rate.
D000653:Amniotic Fluid / Q000166:cytology*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002478:Cells, Cultured; D002842:Chromatids / Q000648:ultrastructure*; D003584:Cytological Techniques; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008937:Mitomycins / Q000494:pharmacology; D008938:Mitosis; D011247:Pregnancy; D013936:Thymidine / Q000494:pharmacology
D008937:Mitomycins; D001973:Bromodeoxyuridine; D013936:Thymidine