Expression of Treponema pallidum antigens in Escherichia coli.
Treponema pallidum DNA was cloned in a bacteriophage. Clones were screened for expression of Treponema pallidum antigens by an in situ radioimmunoassay on nitrocellulose, with the use of subsequent reactions with syphilitic serum and radioiodinated Staphylococcus aureus protein A. One clone, which gave a strong signal, codes for at least seven antigens that react specifically with human antibodies to Treponema pallidum.
D000954:Antigens, Surface / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D003090:Coliphages / Q000235:genetics; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D014210:Treponema pallidum / Q000276:immunology*
D000954:Antigens, Surface; D004274:DNA, Recombinant