[Ultrastructural organization of "Candida albicans" blastospore cell wall: localization of chemical and antigenic components (author's transl)].
Various ultrastructural methods were applied to study the localization of chemical and/or antigenic components of Candida albicans blastospore cell wall. They concerned: PATAg reaction for the detection of polysaccharides on ultrathin sections associated with enzymatic digestions or polysaccharide extraction; the indirect immunoferritin method on intact cells; the indirect immunoperoxidase method on ultrathin section of water soluble embedding medium; the indirect immunofluorescence test, using patients and experimental sera. The cytochemical results confirmed a previously described eight layer organization. The immunocytochemical methods, also in agreement with this stratification, showed in addition to external structures that the layer located near the plasmalemma must be considered as an important antigenic area. The mannans responsible for antigenic differences between strains of C. albicans and namely those supporting the serotype A activity were shown to be, at least, distributed among two of the described peripheral layers.
D000946:Antigens, Fungal / Q000032:analysis*; D002176:Candida albicans / Q000032:analysis / Q000276:immunology / Q000648:ultrastructure*; D002473:Cell Wall / Q000032:analysis; D006651:Histocytochemistry; D007158:Immunologic Techniques; D008351:Mannans / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis*
D000946:Antigens, Fungal; D008351:Mannans; D011134:Polysaccharides
Architecture pariétale des blastospores de Candida albicans: localisation de composants chimiques et antigéniques.