[Application of flow cytometric analysis to brain tumor chemotherapy. Report 1: Changes of cell kinetics induced by anticancer drugs (author's transl)].
It has been reported that flow cytometric analysis offers good indicators to select drugs for chemotherapy of malignant neoplasms, however, there are still few reports to try to apply these data to clinical treatment. For this purpose perturbation of cell cycle traverse which induced by several anticancer drugs was studied to determined the fundamental factors to indicate the sensitivity of each drugs. Results were: 1) Drugs showed high efficiency of accumulation of cells in SG2M phase even in low concentration; MMC, AD, CQ. 2) Drugs showed high efficiency of accumulation of cells in SG2M from low concentration high concentration; Vincristine. 3) Drugs which showed accumulation of SG2M in high concentration; ACNU, BCNU. 9L rat glioma cells treated with BCNU showed high efficiency of accumulation of cells in SG2M phase. In this cell line cytocidal effect was high. However, C6 cells which showed low accumulation in SG2M even by treatment with high concentration of BCNU revealed quite resistant to anticancer drugs from nitrosourea derivatives (ACNU, BCNU). Cytocidal effect of MMC and ACNU on C6 glioma cells was studied by colony forming efficiency assay using multicell spheroids treated with these drugs and it was discussed the correlation between percent of accumulated cells and cytocidal effect of each drug.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects*; D003584:Cytological Techniques; D004273:DNA, Neoplasm / Q000032:analysis; D005910:Glioma / Q000473:pathology*; D007700:Kinetics; D051379:Mice; D008937:Mitomycins / Q000494:pharmacology; D009374:Neoplasms, Experimental / Q000473:pathology; D051381:Rats
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D008937:Mitomycins