title: How to make a hybridoma.
abstract: The use of monoclonal antibodies produced by the hybridoma technique has had a profound impact on the biological and medical sciences. While the general technique for the production of monoclonal antibodies is available from the numerous papers which report their use, there is a need for a source which describes the procedure and its pitfalls in detail so that an investigator who decides to make hybridomas can do so quickly and easily. This guide provides such a source.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002999:Clone Cells; D003584:Cytological Techniques; D006825:Hybridomas; D007158:Immunologic Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D011786:Quality Control
chemical_list: D000911:Antibodies, Monoclonal