title: Induction of mitotic gene conversion by browning reaction products and its modulation by naturally occurring agents.
abstract: Mitotic gene conversion in the D7 strain of Saccharomyces cerevisiae was significantly enhanced by exposure to non-enzymatic browning reaction products. These products were formed during the heating of sugar (caramelization reaction) or sugar-amino acid mixtures (Maillard reaction) at temperatures normally used during the cooking of food. Several modulating factors of this convertogenic activity were identified. These factors included two main groups: (1) trace metals which are widely distributed in the environment; and (2) several cellular enzymatic systems. The convertogenic activities of a heated glucose-lysine mixture and a commercial caramel powder were completely suppresses when yeast were concurrently exposed to these products and to either FeIII or CuII. Equimolar concentrations of MnII or sodium selenite had no effect on the convertogenic activity of the products of either model system. Horse-radish peroxidase, beef liver catalase and rat liver S9 preparations each decreased the frequency of gene conversion induced by the caramel powder and the heated glucose-lysine products. This modulating activity of the enzymes was lost if they were heat-inactivated. These studies indicate the presence of a variety of protective mechanisms which can modify genotoxic components in complex food mixtures.
mesh_terms: D000596:Amino Acids; D005511:Food Handling; D005785:Gene Conversion / Q000187:drug effects*; D005947:Glucose; D006358:Hot Temperature; D008938:Mitosis / Q000187:drug effects*; D010544:Peroxidases / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D013395:Sucrose; D014131:Trace Elements / Q000494:pharmacology*
chemical_list: D000596:Amino Acids; D014131:Trace Elements; D013395:Sucrose; D010544:Peroxidases; D005947:Glucose