title: [Polypeptide synthesis dependent on kinetoplast DNA from Crithidia oncopelti in a cell-free system of E. coli].
abstract: The ability of a complex associate made up of maxi- and mini-circular molecules of kinetoplast DNA from C oncopelti to perform polypeptide synthesis in a cell-free system of conjugate transcription and translation of E. coli was demonstrated. The coding function of maxi-circular molecules and genetic inertness of mini-circular molecules of kinetoplast DNA during polypeptide synthesis in vitro was found. Total RNA from the kinetoplasts was shown to undergo translation in cell-free protein-synthesizing system of E. coli to form 9-10 polypeptides. The molecular weights of many polypeptides, whose synthesis in this system is performed by DNA and RNA from Kinetoplasts were found to be similar.
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D003421:Crithidia / Q000378:metabolism*; D004274:DNA, Recombinant / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
chemical_list: D004274:DNA, Recombinant
vernacular_title: Sintez polipeptidov, zavisimyĭ ot kinetoplastnoĭ DNK Crithidia oncopelti, v beskletochnoĭ sisteme E. coli.