Lethal disruption of the yeast actin gene by integrative DNA transformation.
A mutant allele of the chromosomal locus corresponding to the cloned actin gene of the yeast Saccharomyces cerevisiae has been constructed by DNA transformation with a hybrid plasmid which integrates into, and thereby disrupts, the protein-encoding sequences of the gene. In a diploid strain of yeast, disruption of the actin gene on one chromosome results in a mutation that segregates as a recessive lethal tightly linked to a selectable genetic marker on the integrated plasmid. The actin gene, therefore, must encode an essential function for yeast cell growth.
D000199:Actins / Q000235:genetics* / Q000502:physiology; D000483:Alleles; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D004274:DNA, Recombinant; D005804:Genes, Lethal; D005808:Genes, Recessive; D010957:Plasmids; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic
D000199:Actins; D004271:DNA, Fungal; D004274:DNA, Recombinant