Interactions of Plasmodium falciparum infected erythrocytes with ligand-coated agarose beads.
The binding of normal and Plasmodium falciparum-infected squirrel monkey erythrocytes to columns of ligand-coated agarose beads was compared. Concanavalin A, ricin, soybean and peanut agglutinin-coated beads retain erythrocytes containing large developmental stages of the parasite preferentially to ring-containing erythrocytes or to normal erythrocytes. Binding is inhibited by the sugar corresponding to the lectin's specificity. Preferential binding does not occur with wheat germ or Ulex europaeus agglutinin-coated beads. When infected blood is preincubated in immune serum, infected erythrocytes are specifically retained by Protein A-coated beads. These peculiar binding properties reflect modifications of the infected erythrocyte membrane.
D000373:Agglutinins / Q000378:metabolism; D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D003208:Concanavalin A / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology*; D037102:Lectins / Q000378:metabolism*; D008288:Malaria / Q000097:blood*; D010963:Plasmodium falciparum; D011134:Polysaccharides; D012453:Saimiri; D012685:Sepharose; D013205:Staphylococcal Protein A / Q000378:metabolism
D000373:Agglutinins; D037102:Lectins; D011134:Polysaccharides; D013205:Staphylococcal Protein A; D003208:Concanavalin A; D012685:Sepharose