[In vitro supplementation of pyridoxal phosphate for the optimisation of the determination of the catalytic activity of alanine aminotransferase and aspartate aminotransferase in kidney transplant patients (author's transl)].
Pyridoxal phosphate (PLP) is the coenzyme of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Thus, in vitro supplementation with PLP is important for the optimisation of the determination of catalytic activity of both enzymes. It patients with kidney transplants, stimulation by PLP is very important for ALT activity, which could be affected by plasma PLP deficiency. Furthermore, in this population catalytic activities are more frequently found increased using methods with PLP supplementation than without PLP supplementation (56% and 71% of patients for AST and ALT, respectively). These differences are not related to HBs antigen.
D000293:Adolescent; D000328:Adult; D000375:Aging; D000410:Alanine Transaminase / Q000097:blood*; D001219:Aspartate Aminotransferases / Q000097:blood*; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007674:Kidney Diseases / Q000201:enzymology / Q000628:therapy; D016030:Kidney Transplantation; D008297:Male; D008722:Methods; D008875:Middle Aged; D011732:Pyridoxal Phosphate / Q000494:pharmacology; D013997:Time Factors
D007166:Immunosuppressive Agents; D011732:Pyridoxal Phosphate; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
Intérêt de l'addition in vitro de phosphate de pyridoxal dans la détermination de l'activité catalytique des amino-transférases dans une population de transplantés rénaux.