Aspartate aminotransferase immunoreactivity in cochlea of guinea pig.
The distribution of aspartate aminotransferase-like immunoreactivity in the cochlea of the guinea pig was studied at the light microscopy level. Indirect immunofluorescence histochemistry using antisera against cytoplasmic aspartate aminotransferase prepared from pig heart was applied to surface preparations of the organ of Corti and cryostat sections of the cochlea. In the modiolus, immunofluorescence was localized to spiral ganglion cells and myelinated fibers of the auditory nerve and intraganglionic spiral bundles. In the organ of Corti, immunofluorescence was seen in upper tunnel crossing fibers and at the base of outer hair cells, following a distribution similar to that of the efferent innervation of the outer hair cells. Weak immunofluorescence was seen in the inner spiral bundle and tunnel spiral bundle, but was not present in all preparations. Immunofluorescence was not seen in inner hair cells, nor at the base of inner hair cells, and may have been absent from outer hair cells. It is concluded that spiral ganglion cells and myelinated auditory nerve axons contain aspartate aminotransferase-like immunoreactivity such immunoreactivity has previously been determined in auditory nerve endings inthe cochlear nucleus. Olivocochlear neurons that innervate outer hair cells also contain such immunoreactivity while other cochlear efferents contain little or none.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis* / Q000276:immunology; D003051:Cochlea / Q000201:enzymology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D008297:Male; D009412:Nerve Fibers / Q000201:enzymology; D009476:Neurons, Efferent / Q000201:enzymology; D009925:Organ of Corti / Q000201:enzymology; D013136:Spiral Ganglion / Q000201:enzymology; D000159:Vestibulocochlear Nerve / Q000201:enzymology
D001219:Aspartate Aminotransferases