Improved spatial and temporal resolution in an apparatus for time-lapse phase contrast ciné micrography of cells in vitro.
An apparatus for time-lapse cinemicrography of living animal cells in vitro is described. It has an increased information capacity in comparison with conventional types of equipment in that successful combination of the highest possible spatial resolution with phase contrast microscopy and an improved temporal resolution provided by flash light illumination has been achieved. The interval between exposures of 1/200 s approximately can be reduced to 1/4s using a negative phase-contrast objective of NA 1 . 3. Negative phase-contrast also appeared to be the best technique for imaging of tiny cell surface structures. Thus the new apparatus is suitable for the study of the patterns of cell surface motility in vitro.
D000818:Animals; D002478:Cells, Cultured / Q000648:ultrastructure; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation / Q000379:methods*; D055095:Optics and Photonics; D010784:Photomicrography / Q000295:instrumentation / Q000379:methods*