Immunofluorescence and Treponema infection: a method using immunofluorescence to study rabbit testicular tissue infected with T pallidum and T pertenue.
We compared immunofluorescent staining of rabbit testicular tissue infected with Treponema pallidum or T pertenue, and fixed in Bouin's fixative, 95% cold ethyl alcohol with 1% glacial acetic acid, or routine 10% buffered formalin solution. The fixative of choice clearly was Bouin's. Although we studied only rabbit tissue, we assume that these fixatives will work well in human biopsy or autopsy material when identification of pathogenic Treponema is needed.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D005455:Fluorescent Antibody Technique; D008297:Male; D011817:Rabbits; D013737:Testis / Q000382:microbiology*; D014208:Treponema / Q000276:immunology*; D014210:Treponema pallidum / Q000302:isolation & purification; D014211:Treponemal Infections / Q000382:microbiology*
D000942:Antigens, Bacterial