T cell replacing factor substitutes for an I-J+ idiotype-specific T helper cell.
An in vitro system for the study of idiotype (Id) expression on antitrimethylamino hapten antibody-producing cells and its regulation by two classes of helper T cells is described. These cells are distinguished in four ways: one requires a hapten-carrier bridge and gives a good response that is low in Id; it does not bind to Id-coated dishes and is not affected by anti-I-J plus complement. The other requires antigen but not a hapten-carrier bridge, is bound by Id-coated dishes and is killed by anti-I-J and complement. The Id-specific cell appears to be antigen specific and acts via a soluble factor(s).
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D000950:Antigens, Ly / Q000276:immunology; D003429:Cross Reactions; D006462:Hemolytic Plaque Technique; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D015848:Interleukin-5; D008222:Lymphokines / Q000494:pharmacology*; D008297:Male; D008744:Methylamines / Q000276:immunology; D051379:Mice; D008805:Mice, Inbred A; D014176:Protein Biosynthesis; D013601:T-Lymphocytes / Q000276:immunology*
D000950:Antigens, Ly; D007130:Immunoglobulin Idiotypes; D015848:Interleukin-5; D008222:Lymphokines; D008744:Methylamines; C023544:phenyltrimethylamino hapten