Detection of surface immunoglobulins of human lymphoid cells: a comparative study of live and fixed cells using a direct immunoperoxidase procedure.
Surface immunoglobulins (Ig) of normal and malignant lymphoid cells were detected on prefixed, smeared (method A) and live (method B) cell suspensions; the results were compared with regard to staining patterns, specificity and sensitivity. In both methods surface Ig were detected by a direct immunoperoxidase procedure using conjugated purified antibody. Although method A has practical advantages, method B is more sensitive. The reasons for this discrepancy are discussed in relation to surface Ig denaturation and redistribution.
D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D006801:Humans; D007124:Immunoenzyme Techniques; D008221:Lymphoid Tissue / Q000276:immunology*; D008232:Lymphoproliferative Disorders / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*
D011947:Receptors, Antigen, B-Cell