[Enzyme immunoassay (EIA) for the estrogenic stilbene derivative diethylstilbestrol (DES) (author's transl)].
An enzyme immunoassay (EIA) for diethylstilbestrol (DES) is described, which is based on the competitive reaction between DES and peroxidase-labelled DES for an antibody, which has been bond to an insoluble support. The preparation of the enzyme-labelled DES necessitates the use of highly purified 4-O-(carboxypropyl)-diethylstilbestrol (CP-DES) to obtain a highly immunoreactive conjugate. CP-DES was synthesized from DES and ethyl-4-bromobutylate and was purified by a novel isolation procedure involving a simple solvent partition followed by hydrolysis of the ester. It was characterized by it's chemical and biological properties. The assay covers the range from 0.2 to 20 pmol/test (approximately equal to 0.05-5 ng/test). It has sufficient sensitivity and specificity compared with radio immuno assay to make it a potential method for controlling the misuse of this estrogenic substance in animal production.
D000818:Animals; D003429:Cross Reactions; D004054:Diethylstilbestrol / Q000032:analysis* / Q000494:pharmacology; D005260:Female; D007124:Immunoenzyme Techniques; D008099:Liver / Q000032:analysis; D051379:Mice; D008832:Microchemistry; D011863:Radioimmunoassay; D014590:Uterine Contraction / Q000187:drug effects; D014599:Uterus / Q000187:drug effects
D004054:Diethylstilbestrol
Enzym-Immuntest (EIA) für das synthetische Ostrogen Diäthylstilböstrol (DES).