title: Induction of autoantibody-producing cells after the coculture of haptenated and normal human mononuclear leukocytes.
abstract: The coculture of normal human peripheral blood mononuclear leukocytes (PBL) and autologous mononuclear leukocytes coupled to the trinitrophenyl (TNP) hapten (TNP-PBL) was found to induce a polyclonal activation of antibody-producing cells. The polyclonal activation of antibody-producing cells was demonstrated by detecting the induction of cells producing antibody to sheep red blood cells using a complement-dependent, direct, hemolytic plaque-forming cell (PFC) assay. A ratio of four normal to one haptenated mononuclear leukocyte was found to be optimal for inducing the polyclonal activation of antibody-producing cell in these cultures. The plaque-forming cells assay in these experiments utilized monolayers of indicator red cells. Further evidence for the polyclonal induction of antibody-producing cells by TNP-PBL was provided by demonstrating PFC on monolayers of not only sheep red blood cells, but also autologous human red cells, bromelain-treated autologous red cells, TNP-coupled human and sheep red cells, and human autologous red cells coupled to human heat-aggregated IgG with chromic chloride. Thus cells secreting antibody to TNP, human red cells, and human IgG were induced. Anti-IgG and anti-human red cell-producing cells were first detected on Day 2 of culture and were still present on Day 9. Mononuclear leukocytes altered by chemical haptenation polyclonally stimulate normal mononuclear leukocytes to become antibody-producing cells. This polyclonal stimulation of antibody-producing cells includes cells producing antibodies to human IgG and human autologous red blood cells suggesting that autoantibody-producing cells are induced.
mesh_terms: D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D001323:Autoantibodies / Q000096:biosynthesis*; D001963:Bromelains / Q000494:pharmacology; D002478:Cells, Cultured; D004912:Erythrocytes / Q000276:immunology; D006241:Haptens / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D009596:Nitrophenols / Q000276:immunology; D012217:Rheumatoid Factor / Q000276:immunology; D012756:Sheep
chemical_list: D001323:Autoantibodies; D006241:Haptens; D007074:Immunoglobulin G; D009596:Nitrophenols; D001963:Bromelains; D012217:Rheumatoid Factor