[Regulation of energy exchange in synchronous cultures of E. coli in the chemostat].
A modified method of chemical synchronization of E. coli culture with impulse additions of phosphate is presented, devices for automatic analysis of morphological and biochemical parameters of the bacterial culture are described. A synchronous cell division was obtained under flow-type regime, the synchronization degree reaching 60%. Under synchronous division of E. coli cells a correlation between the culture titre and variations in O2, CO2, phosphate concentrations and in the activity of acid phosphatase were observed. The results obtained point to the phosphate-dependent periodical work of the power supply system of the E. coli cells in the course of the cell cycle.
D000135:Acid Phosphatase / Q000378:metabolism; D002455:Cell Division; D003470:Culture Media; D004734:Energy Metabolism; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D007700:Kinetics; D010101:Oxygen Consumption; D010710:Phosphates / Q000378:metabolism
D003470:Culture Media; D010710:Phosphates; D000135:Acid Phosphatase
Issledovanie reguliatsii énergoobmena v sinkhronnoĭ kul'ture E. coli v usloviiakh khemostata.