Immunohistochemistry of invasion in threedimensional culture.
Phagocytosis of material from the host tissue by invading malignant cells is suspected from the presence of vacuoles containing unidentified debris. Experiments with 51Cr-labeled host tissues in vitro support the role of phagocytosis during invasion. Using the unlabeled antibody enzyme method material from embryonic chick heart inside invading MO4 cells, HeLa cells and B 16 melanoma cells in threedimensional cultures is stained with a rabbit antiserum against embryonic chick heart. These observations show that phagocytosis by invading cells in vitro is at least partly responsible for the disappearance of the host tissue.
D000818:Animals; D002642:Chick Embryo; D006367:HeLa Cells / Q000648:ultrastructure; D007124:Immunoenzyme Techniques; D008545:Melanoma / Q000648:ultrastructure; D008854:Microscopy, Electron; D009361:Neoplasm Invasiveness / Q000648:ultrastructure*; D009374:Neoplasms, Experimental / Q000648:ultrastructure; D010587:Phagocytosis