[Conjugation transfer of chromosome markers in an Erwinia chrysanthemi bacterial system. I. Construction of a strain capable of transferring the chromosome during conjugation].
The donor strain Erwinia chrysanthemi VY1-10, capable of transferring chromosomal markers at a high frequency (4,7.10(-5)-1,8.10(-3) in crosses with the isogenic polyauxotrophic recipient strain E. chrysanthemi VY7 lac, thr1, leu1, pro1, his2, str-r, was obtained from the strain E. chrysanthemi ENA49 Flac+ (VY1) after growth at 28 degrees on the medium containing acridine orange. Furthermore, the cells of the donor obtained are characterized by stable inheritance of lac+ character.
D002876:Chromosomes, Bacterial / Q000648:ultrastructure*; D003227:Conjugation, Genetic; D004885:Erwinia / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005819:Genetic Markers; D009154:Mutation; D010957:Plasmids
D005819:Genetic Markers
Kon"iugatsionnaia peredacha khromosomal'nykh markerov v sisteme bakterii Erwinia chrysanthemi. Soobshchenie I. Konstruirovanie shtamma, sposobnogo peredavat' khromosomu pri kon"iugatsii.