title: C-peptide-measurement: a simple method for the improvement of specificity.
abstract: Sera containing insulin antibodies and proinsulin give artifactual high readings in C-Peptide measurement. This problem can be circumvented by the removal of antibodies and proinsulin prior to C-Peptide assay. A method is described which enables the removal of antibodies and proinsulin from sera without affecting the C-Peptide determination. Furthermore, this method allows a semiquantitative estimation of proinsulin, provided there is enough cross reactivity of the C-Peptide antibody with proinsulin.
mesh_terms: D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D002096:C-Peptide / Q000276:immunology; D003429:Cross Reactions; D006168:Guinea Pigs; D006801:Humans; D007328:Insulin / Q000276:immunology; D010455:Peptides; D011092:Polyethylene Glycols / Q000494:pharmacology; D011384:Proinsulin
chemical_list: D000906:Antibodies; D002096:C-Peptide; D007328:Insulin; D010455:Peptides; D011092:Polyethylene Glycols; D011384:Proinsulin