A simple one-step purification of human alpha 1-proteinase inhibitor by immunoadsorbent column chromatography.
A one-step purification of human alpha 1-proteinase inhibitor was described using the rabbit anti-alpha 1-proteinase inhibitor antibody coupled to CNBr-activated Sepharose 4B. The elution of alpha 1-proteinase inhibitor from the immunoadsorbent column using 0.1 M Na2CO3/0.5 M NaCl solution gave an 85% yield. The properties of eluted alpha 1-proteinase inhibitor were identical with that of alpha 1-proteinase inhibitor that was purified by the conventional method. In addition, the specific activity of purified alpha 1-proteinase inhibitor was more than 93% of that of the theoretical value.
D002847:Chromatography, Agarose; D006801:Humans; D007163:Immunosorbent Techniques; D011480:Protease Inhibitors / Q000097:blood; D000515:alpha 1-Antitrypsin / Q000302:isolation & purification*
D011480:Protease Inhibitors; D000515:alpha 1-Antitrypsin