Rapid purification of extracellular and intracellular Moloney murine leukemia virus.
The present study demonstrates the advantages of a combination of concentration by polyethylene glycol-6000 and Sepharose Cl-4B chromatography as a rapid procedure for retroviruses purification. This procedure can be completed within 3 hours, providing a high degree of virus purification with minimal damage to its structural and biological properties. Using transmission electron microscopy we observed many intracellular type-C virions in cytoplasmic vacuoles of 3T3/NIH cells chronically infected with Moloney murine leukemia virus. There intracellular virions could be isolated from postmitochondrial cytoplasmic fractions prepared from the infected cells by a procedure which minimized its contamination by extracellular free or membrane-bound virions. SDS-polyacrylamide gel electrophoresis showed that the intracellular and extracellular virions contained similar protein composition.
D000818:Animals; D002478:Cells, Cultured; D002850:Chromatography, Gel; D051379:Mice; D008828:Microbiological Techniques; D008979:Moloney murine leukemia virus / Q000302:isolation & purification* / Q000648:ultrastructure; D011092:Polyethylene Glycols; D014764:Viral Proteins / Q000032:analysis
D014764:Viral Proteins; D011092:Polyethylene Glycols