Cloning and expression in Escherichia coli K-12 of the genes for major outer membrane protein OmpA from Shigella dysenteriae, Enterobacter aerogenes, and Serratia marcescens.
The outer membranes of many gram-negative bacteria contain a major heat-modifiable protein which shows serological cross-reactivity with the OmpA protein of Escherichia coli K-12. Using the cloned gene for the E. coli K12 protein as a DNA-DNA hybridization probe, we were able to identify the corresponding genes from Shigella dysenteriae. Enterobacter aerogenes, and Serratia marcescens. These were cloned in a phage lambda vector, and their expression in E. coli K-12 was studied. All three OmpA proteins were fully produced and correctly exported to the outer membrane. In several cases, complete or partial restoration of known function of the E. coli K-12 protein was observed.
D001425:Bacterial Outer Membrane Proteins; D003001:Cloning, Molecular; D003087:Colicins / Q000502:physiology; D003227:Conjugation, Genetic; D004754:Enterobacter / Q000235:genetics; D004755:Enterobacteriaceae / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D012706:Serratia marcescens / Q000235:genetics; D012762:Shigella dysenteriae / Q000235:genetics
D001425:Bacterial Outer Membrane Proteins; D003087:Colicins; D008565:Membrane Proteins