Ultrastructural localization of type V collagen in rat kidney.
Antibodies specific for the alpha 1 (V) chain and native collagen molecules containing the alpha 1 (V) chain have been used in electron immunohistochemical studies of rat kidney to determine the ultrastructural distribution of this class of collagen molecules. In addition, antibodies against type I collagen and whole basement membrane were used as markers for interstitial collagen and authentic basement membranes. Our results indicate that type V collagen is present in the renal interstitium in different forms: in close apposition to interstitial collagen fibers; in the stromal aspect of vascular basement membranes; and as particulate material not bound to other structures. On the basis of these findings, we postulate a binding or connecting function for this collagen type.
D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure; D003094:Collagen / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D005110:Extracellular Space / Q000502:physiology; D007158:Immunologic Techniques; D007668:Kidney / Q000648:ultrastructure*; D051381:Rats
D003094:Collagen