Glucose transport and oxidation in adipose tissue of patients with myotonic dystrophy.
The effect of insulin on the transport of 2-deoxyglucose and the oxidation of glucose in chopped adipose tissue was investigated in 14 myotonic dystrophy (MyD) patients and 28 age and size-matched control subjects. The transport of 0.55 mM 2-deoxyglucose was measured over 3 min at 37 degrees C both with and without 32 ng/ml of insulin. Oxidation was determined at 37 degrees C for 90 min by the measurement of 14CO2 released from a system containing 0.55 mM glucose with and without 50 ng/ml of insulin. Basal 2-deoxyglucose transport was not reduced in MyD subjects but insulin-stimulated 2-deoxyglucose transport in MyD was significantly less at 0.512 +/- 0.220 nmole compared to control subjects with 0.906 +/- 0.160 nmole/100 mg tissue/3 min (P less than 0.02). Both the basal and insulin-stimulated glucose oxidation were significantly less in the MyD group. Insulin-stimulated oxidation was 2.92 +/- 0.21 nmole in the control subjects compared to 2.20 +/- 0.27 nmole/100 mg tissue/90 min in the MyD cases (P less than 0.02). Similar findings were obtained when calculations were based on nmoles of 2-deoxyglucose transport and glucose oxidation/100 mg lipid. The findings indicate that both glucose transport and oxidation are impaired in MyD.
D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D003847:Deoxyglucose / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D008875:Middle Aged; D009223:Myotonic Dystrophy / Q000378:metabolism*; D010084:Oxidation-Reduction; D013997:Time Factors
D007328:Insulin; D003847:Deoxyglucose; D005947:Glucose