In vitro colon assay of peripheral blood mononuclear cells - a comparison of breast cancer patients and normal individuals.
The in vitro colony growth in agar of peripheral blood mononuclear cells (PBMC) with phytohemagglutinin (PHA) as a stimulant is on an average lower in breast cancer patients than in normal individuals of the same age and sex group. This difference is statistically significant in patients with stage 1 disease but not in those with stage 2 or stage 4 disease. It is also noted that in normal individuals the in vitro colony growth of PBMC stimulated with PHA alone is invariably greater than that obtained with PHA combined with phorbol myristate acetate as mitogens. Among stage 1 breast cancer patients there is a group in which this pattern is reversed. No distinguishing features in this group can as yet be identified.
D001943:Breast Neoplasms / Q000276:immunology*; D002999:Clone Cells; D005260:Female; D006801:Humans; D007158:Immunologic Techniques; D008214:Lymphocytes / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D010835:Phytohemagglutinins; D013755:Tetradecanoylphorbol Acetate