title: Countercurrent chromatography.
abstract: Countercurrent chromatography is a newly developed versatile partition chromatography which totally eliminates the use of solid supports. The method utilizes the intriguing hydrodynamic behavior of two immiscible solvents in a rotating coiled tube. Various types of seal-free flow-through centrifuge schemes are introduced to facilitate a continuous elution process. The method yields high partition efficiencies comparable to liquid chromatography but without the complications arising from the use of solid supports. Countercurrent chromatography covers a wide spectrum of applicable samples ranging from small ions and molecules to macromolecules and even cell particles in both analytical and preparative scales.
mesh_terms: D000054:Acceleration; D000818:Animals; D002469:Cell Separation; D002498:Centrifugation / Q000295:instrumentation; D002845:Chromatography / Q000295:instrumentation / Q000379:methods; D003377:Countercurrent Distribution / Q000295:instrumentation; D004912:Erythrocytes / Q000166:cytology; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008433:Mathematics; D012399:Rotation