Nucleotide insertion in the anticodon loop of a glycine transfer RNA causes missense suppression.
We have determined the nucleotide sequences of two unusual UGG-suppressing glycine tRNAs from Escherichia coli and, as a result, have discovered a new mechanism for the generation of missense suppressors. The suppressor tRNAs translate UGG but not UGA. Each arose as a consequence of spontaneous mutational alteration of glyT, the gene for the GGA/G-reading glycine tRNA of E. coli. In each mutant tRNA, the change in primary structure involved the insertion of an adenylate residue on the 3' side of the anticodon and the loss of a modification of the uridylate residue at the 5' end of the anticodon. A "shift" of the effective anticodon by one nucleotide in the 3' direction can account for the new coding specificity of these tRNAs.
D000926:Anticodon; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D005998:Glycine; D006860:Hydrogen Bonding; D012343:RNA, Transfer / Q000235:genetics*; D013489:Suppression, Genetic
D000926:Anticodon; D012343:RNA, Transfer; D005998:Glycine