Surfactant phosphatidylcholine source, fluxes, and turnover times in 3-day-old, 10-day-old, and adult rabbits.
We have measured turnover times for alveolar surfactant phosphatidylcholine in rabbits at 3 and 10 days of age and in adult rabbits. To generate accurate estimates of the turnover time of surfactant phosphatidylcholine from lamellar bodies into the alveolar space, large numbers of rabbits at each age were injected with radiolabeled choline, palmitic acid, and phosphate. Phosphatidylcholine was isolated from lamellar body and surfactant fractions from each rabbit. Curves of specific activity versus time were analyzed using the Zilversmit equations for a two compartment precursor-product model. The curves for each labeled precursor at each age were consistent with the lamellar bodies being the sole precursors of surfactant phosphatidylcholine. The same equations were used to calculate turnover times for surfactant phosphatidylcholine; these were 8-10 h in 3- and 10-day-old rabbits but only 3 h in adult rabbits. From estimates of surfactant phosphatidylcholine pool sizes and turnover times, the flux of surfactant phosphatidylcholine was estimated to be 3.4 mumol/h in adult and 0.48 mumol/h in 3-day-old rabbits; however, the flux expressed/kg of body weight is 2.4 times larger in 3-day-old than in adult rabbits. Finally, the conservation of the radiolabeled precursors in phosphatidylcholine implies reutilization of phosphatidylcholine after synthesis and secretion.
D000375:Aging; D000818:Animals; D002250:Carbon Radioisotopes; D002794:Choline / Q000378:metabolism; D007700:Kinetics; D008433:Mathematics; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000254:growth & development* / Q000378:metabolism; D011663:Pulmonary Surfactants / Q000378:metabolism*; D011817:Rabbits; D011865:Radioisotope Dilution Technique; D014316:Tritium
D002250:Carbon Radioisotopes; D010169:Palmitic Acids; D011663:Pulmonary Surfactants; D014316:Tritium; D019308:Palmitic Acid; D002794:Choline