[LeuB24]- and [LeuB25]insulins are not antagonists of lipogenesis in adipocytes.
Semisynthetic human [LeuB24]-and [LeuB25]insulins were investigated to determine whether they show antagonistic properties towards insulin-stimulated lipogenesis in isolated fat cells. In contrast to other reports, we could detect only an additive agonistic effect when constant concentrations (e.g. 0.3 ng/ml) of the analogues were mixed with varying concentrations of insulin, or when constant concentrations (e.g. 0.3 ng/ml, 0.6 ng/ml) of insulin were mixed with varying concentrations of the analogues. Similar results were obtained with mixtures of insulin and NA2-acetyl- or NA2-propionyl-des-GlyA1-insulins. These results do not support the contention that a diabetic state could be caused by either of these mutant human insulins.
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000031:analogs & derivatives* / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis*; D010084:Oxidation-Reduction
D007328:Insulin; D008055:Lipids; C031725:insulin, Leu(B25)-; C031724:insulin, Leu(B24)-; D005947:Glucose