Improved immunohistochemical visualization of central serotonin nerves after loading with 5,7-dihydroxytryptamine.
5,7-Dihydroxytryptamine (5,7-DHT), which reacts with a monoclonal antibody raised against 5-hydroxytryptamine (5-HT), has been used to increase the sensitivity of the immunohistochemical detection by this antibody of central 5-HT nerves in the rat brain. Rats pretreated with tricyclic antidepressants were given a single intraventricular injection of 5,7-DHT and were sacrificed 30 min-3 h later. This treatment resulted in accumulation of 5,7-DHT by central 5-HT nerves and selectively enhanced the immunofluorescence reaction of these nerves, particularly the terminals and non-terminal fibres.
D015116:5,7-Dihydroxytryptamine; D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000166:cytology; D002490:Central Nervous System / Q000033:anatomy & histology*; D004102:Dihydroxytryptamines; D006651:Histocytochemistry; D007158:Immunologic Techniques; D008526:Medulla Oblongata / Q000166:cytology; D009434:Neural Pathways / Q000033:anatomy & histology*; D009474:Neurons / Q000166:cytology; D051381:Rats; D012701:Serotonin / Q000502:physiology*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D004102:Dihydroxytryptamines; D015116:5,7-Dihydroxytryptamine; D012701:Serotonin