Genetic studies concerning the mechanism of secretion of the maltose-binding protein of Escherichia coli.
Strains of Escherichia coli have been isolated in which the malE-gene encoding the periplasmic maltose-binding protein has been used to the lacZ-gene encoding the cytoplasmic enzyme beta-galactosidase. Certain of these strains synthesize malE-lacZ hybrid proteins that retain beta-galactosidase enzyme activity. The unusual properties of these fusion strains, at variance with our original predictions, have provided us the means to initiate a detailed genetic analysis of the protein export process.
D018528:ATP-Binding Cassette Transporters; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D007700:Kinetics; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D009154:Mutation; D033902:Periplasmic Binding Proteins; D010641:Phenotype
D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C461619:MalE protein, E coli; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; C461599:maltose transport system, E coli