Production and evaluation of antibody to the heat-stable enterotoxin from a human strain of enterotoxigenic Escherichia coli.
Escherichia coli heat-stable enterotoxin was coupled to bovine serum albumin by a carbodiimide reagent. Antibody to the conjugate was produced by immunization of rabbits. Data from radioimmunoassay and infant mouse tests indicate the presence of antibody to the enterotoxin. The antisera can be used in a radioimmunoassay to measure enterotoxin in various fluids.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000936:Antigen-Antibody Complex / Q000032:analysis; D001666:Binding Sites, Antibody; D004768:Enterotoxins / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology*; D007114:Immunization; D051379:Mice; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine / Q000276:immunology
D000907:Antibodies, Bacterial; D000936:Antigen-Antibody Complex; D004768:Enterotoxins; D012710:Serum Albumin, Bovine