title: Modified nutrient medium MCDB 151, defined growth factors, cholera toxin, pituitary factors, and horse serum support epithelial cell and suppress fibroblast proliferation in primary cultures of rat ventral prostate cells.
abstract: Nutrient medium WAJC 401 containing 5 micrograms/ml insulin. 10 ng/ml EGF, 10 ng/ml cholera toxin, 50 micrograms/ml pituitary extract, 1 microgram/ml prolactin, 1 microM dexamethasone, and 5% horse serum supports the rapid proliferation of rat ventral prostate epithelial cells in primary culture. The same medium suppresses the growth of prostate fibroblasts.
mesh_terms: D000818:Animals; D001769:Blood; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology*; D003470:Culture Media; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D006133:Growth Substances / Q000494:pharmacology*; D006736:Horses; D007328:Insulin / Q000494:pharmacology; D008297:Male; D010902:Pituitary Gland / Q000502:physiology*; D011467:Prostate / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D014020:Tissue Extracts / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D006133:Growth Substances; D007328:Insulin; D014020:Tissue Extracts; D004815:Epidermal Growth Factor; D002772:Cholera Toxin