title: Kinetics of c2-repressor synthesis in a regulatory defective P22 mutant.
abstract: Phage P22 defective in gene 24 and harbouring the Oc mutation k5 in OR exhibits a strongly increased c2-repressor synthesis after infection of non-lysogenic S. typhimurium. The repressor synthesis depends strictly on an intact c1 gene. The kinetics of its synthesis, as monitored by polyacrylamide gel electrophoresis, is the same as with P22 c+, namely a turn off 8-10 min after infection. - After infection of P22-lysogenic bacteria with either P22 24- k5 or P22 24- k5 c1, much lower amounts of repressor are synthesized but again with the same kinetics. These results suggest a cro-like function acting at PRE and PRM of P22. The possible reason for the c2 overproduction is discussed.
mesh_terms: D005814:Genes, Viral; D007700:Kinetics; D008242:Lysogeny; D009154:Mutation; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics*; D012483:Salmonella Phages / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D012097:Repressor Proteins; D014157:Transcription Factors; D014764:Viral Proteins