A novel extracellular proteinase from Bacillus pumilus.
Bacillus pumilus NCTC AII6/73 produces a novel extracellular alkaline proteinase whose optimum is pH 9.5. The purified product from ion-exchange chromatography (DEAE-cellulose) was resolved into two bands by polyacrylamide-sodium dodecyl sulphate (SDS) gel electrophoresis. These had approximate molecular weights of 15.6 x 10(3) daltons, respectively, and a high content of glycine and proline, comprising roughly 50% of the total amino residues.
D000596:Amino Acids / Q000032:analysis; D001407:Bacillus / Q000201:enzymology*; D055598:Chemical Phenomena; D002621:Chemistry; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration
D000596:Amino Acids; D010450:Endopeptidases