title: Ribosomal recessive suppressors cause a respiratory deficiency in yeast Saccharomyces cerevisiae.
abstract: Recessive suppressor mutations in yeast Saccharomyces cerevisiae alter a component of the cytoplasmic ribosomes, relaxing the control of translational fidelity. As a consequence ribosomes can misread nonsense codons as amino acids (Surguchov et al. 1980a). The suppressor mutants are often respiratory deficient, being unable to grow on non-fermentable substrates. The study of the cytochrome spectra has revealed that the cytochrome b and aa3 contents were lower in the mutants than in the parent strains. Furthermore, the suppressor mutations often cause hypersensitivity to paromomycin and neomycin on media with a non-fermentable source of carbon. Some of the suppressor mutants exhibited both erythromycin and chloramphenicol-dependent growth on media containing ethanol or glycerol as a sole carbon source. These results suggest that the mutations altering cytoplasmic ribosomes may simultaneously impair the mitochondrial translation. A coupling of cytoplasmic and mitochondrial protein synthesis in yeast cells is proposed. The existence of a common protein component participating both in mitochondrial and cytoplasmic protein synthesis apparatus is discussed.
mesh_terms: D003580:Cytochromes / Q000032:analysis; D004734:Energy Metabolism; D005808:Genes, Recessive; D008540:Meiosis; D008928:Mitochondria / Q000502:physiology; D008938:Mitosis; D010101:Oxygen Consumption; D012270:Ribosomes / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D013057:Spectrum Analysis; D013489:Suppression, Genetic
chemical_list: D003580:Cytochromes