The reconstitution of binding protein-dependent active transport of glutamine in isolated membrane vesicles from Escherichia coli.
The reconstitution of binding protein-dependent glutamine transport in isolated membrane vesicles from Escherichia coli is described. The reconstituted glutamine transport is shown to be energy-dependent and does not involve the metabolism of glutamine or the trapping of liganded binding protein with the vesicles. The preparation of vesicles capable of transporting glutamine in a binding protein-dependent manner required the incorporation of NAD into vesicles and the use of a binding protein point mutant as the source of the vesicles.
D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D007700:Kinetics; D009243:NAD / Q000378:metabolism
D002352:Carrier Proteins; D005973:Glutamine; D009243:NAD