title: Biosynthesis of radiolabeled verruculogen by Penicillium simplicissimum.
abstract: In surface culture of Penicillium simplicissimum, verruculogen was shown to be biosynthesized from the intact carbon skeletons of tryptophan and proline, isoprenoid derivatives of mevalonic acid, and a methyl group donated by methionine. Selected radiolabeled precursors (1 mCi) pulse-fed at the optimum stage of fermentation yielded verruculogen (specific activity, 5.89 X 10(2) microCi mmol-1) labeled in the prolyl and isoprenyl regions of the molecule and suitable for metabolic studies.
mesh_terms: D002250:Carbon Radioisotopes; D007211:Indoles / Q000096:biosynthesis*; D007700:Kinetics; D009183:Mycotoxins / Q000096:biosynthesis*; D010407:Penicillium / Q000378:metabolism*; D011865:Radioisotope Dilution Technique
chemical_list: D002250:Carbon Radioisotopes; D007211:Indoles; D009183:Mycotoxins; C009658:verruculogen