Rate of elongation of polyphenylalanine in vitro.
Ribosomes purified from Escherichia coli were preincubated with AcPhe-tRNA and poly(U). Then purified components necessary for polypeptide synthesis were added. Incubation of the complete system led to a burst of elongation which lasted for nearly 10 s. During the initial burst approximately 10% of the ribosomes participated in the elongation of poly(Phe) at an average rate per ribosome close to eight peptide bonds/s. The missense error rate with leucine was 4 x 10(-4) during the burst. Accordingly, the preincubated elongation system functions at a rate, as well as an accuracy, close to those of protein synthesis in vivo.
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D010452:Peptide Biosynthesis; D010441:Peptide Chain Elongation, Translational; D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors / Q000494:pharmacology; D010455:Peptides; D011072:Poly U / Q000378:metabolism; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl; D012270:Ribosomes / Q000378:metabolism; D013997:Time Factors
D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors; D010455:Peptides; D012346:RNA, Transfer, Amino Acyl; C006383:tRNA, N-acetylphenylalanine-; D011072:Poly U; C012001:polyphenylalanine; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D007930:Leucine