Altered expression of biodegradative threonine dehydratase in Escherichia coli mutants.
A number of strains of Escherichia coli K-12 failed to synthesize significant amounts of biodegradative threonine dehydratase (EC 4.2.1.16) when grown anaerobically in tryptone-yeast extract medium, a condition which is optimal for the induction of this enzyme. However, the addition of 10 mM potassium nitrate to the culture medium enabled a few of these strains, notably MB201, to induce the enzyme. An examination of the kinetic parameters, modifier sensitivity, and immunological cross-reactivity revealed that the enzyme produced by MB201 in nitrate-supplemented medium appeared indistinguishable from the dehydratase of a wild-type strain. The reduced expression of threonine dehydratase in MB201 appeared highly specific; the synthesis of two other inducible enzymes, D-serine deaminase and tryptophanase, and two "anaerobic" proteins, namely, fumarate reductase and cytochrome c551, remained unaffected. The mutation (tdcI) responsible for the altered expression of the dehydratase in MB201 was located at min 91 on the E. coli chromosome and appeared to tightly linked to if not identical with pgi, the gene encoding phosphoglucose isomerase, as judged by growth experiments on glucose and fructose, direct assay of phosphoglucose isomerase activity, spontaneous and simultaneous reversion of MB201 (tdcI) to TdcI+ and Pgi+ phenotype, and cosegregation of the two loci during transduction with P1 phage. Because not all strains lacking the dehydratase showed nitrate-dependent enzyme synthesis or had lesions at the pgi locus, it appears that mutations at multiple loci on the E. coli chromosome may influence the expression of the enzyme in vivo.
D000249:Adenosine Monophosphate / Q000494:pharmacology; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology*; D005798:Genes, Bacterial; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D012695:L-Serine Dehydratase / Q000096:biosynthesis; D009154:Mutation; D009566:Nitrates / Q000494:pharmacology; D017680:Potassium Compounds; D013913:Threonine Dehydratase / Q000096:biosynthesis*; D014368:Tryptophanase / Q000096:biosynthesis
D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D009566:Nitrates; D017680:Potassium Compounds; D000249:Adenosine Monophosphate; D014368:Tryptophanase; D012695:L-Serine Dehydratase; D013913:Threonine Dehydratase; C023844:potassium nitrate