Peripheral nerve autografts increase soleus muscle hydrolase activity.
A peripheral nerve autograft placed on the surface of a normally innervated muscle has been shown to induce acetylcholine hypersensitivity and myofibrillar degeneration. Using a similar preparation, we determined the acid protease, alkaline protease, and N-acetylglucosaminidase activity in rat soleus muscle and the effect of protease inhibitors on these enzymes. Three days after nerve transplant, the muscle showed a significant increase in all three enzymes assayed. The protease inhibitors leupeptin and pepstatin totally prevented the nerve-induced increase in hydrolase activity. Our data suggest that muscle degeneration is secondary to an inflammatory response induced by the nerve.
D000118:Acetylglucosaminidase / Q000378:metabolism*; D000818:Animals; D006596:Hexosaminidases / Q000378:metabolism*; D007976:Leupeptins; D008297:Male; D009132:Muscles / Q000201:enzymology* / Q000294:innervation; D010436:Pepstatins / Q000494:pharmacology; D010447:Peptide Hydrolases / Q000378:metabolism*; D010525:Peripheral Nerves / Q000637:transplantation*; D011480:Protease Inhibitors / Q000494:pharmacology; D051381:Rats; D013997:Time Factors
D007976:Leupeptins; D010436:Pepstatins; D011480:Protease Inhibitors; D006596:Hexosaminidases; D000118:Acetylglucosaminidase; D010447:Peptide Hydrolases