Dunning adenocarcinoma in tissue culture: isolation of a cloned cell line, R3327H-G8-A1.
A cloned cell line, R3327H-G8-A1, was isolated from an explant of the well differentiated androgen dependent Dunning R3327H adenocarcinoma. Preliminary characterization of this cell line indicates that it is epithelial-like, and that it synthesizes and secretes large quantities of acid phosphatase. The cells bind testosterone in a saturable manner with an equilibrium dissociation constant of 0.5 nM and with a capacity of 5000 sites/cell. When these cells were injected subcutaneously into the hind flank of Copenhagen-Fischer rats, or into the dorsal or ventral lobes of the prostate, large tumors were produced 3 months following administration, thus demonstrating the tumorigenicity of the cells.
D000135:Acid Phosphatase / Q000378:metabolism; D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D008297:Male; D008858:Microscopy, Phase-Contrast; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000378:metabolism
D013739:Testosterone; D000135:Acid Phosphatase