A nonspecific inhibitor of macrophage migration in cell-free ascites of rat Zajdela hepatoma. II. Biochemical characterization and purificaton.
Macrophage migration inhibitory activity from Zajdela hepatoma ascites (AH-MIF) was characterized by ion exchange chromatography, gel filtration and isoelectric focusing. The data obtained indicate molecular heterogeneity according to net charge as revealed by chromatography on DEAE-Sephadex A-50. Furthermore, molecular sieve chromatography with Sephadex G-100 demonstrated the existence of subfractions with molecular weights of about 45,000, 20,000 and 10,000 - 15,000 respectively. Isoelectric focusing revealed further heterogeneity of a subfraction (10,000 - 15,000) eluting at 0.17 - 0.25 M NaCl by ion exchange chromatography. The migration inhibitory factor(s) from Zajdela hepatoma ascites were shown to adsorb to alpha-L-fucose-Sepharose 6B columns. The relationships of AH-MIF to functionally similar tumor-derived factors and to lymphocyte-derived conventional MIF ar discussed.
D000818:Animals; D002846:Chromatography, Affinity; D005643:Fucose; D007525:Isoelectric Focusing; D008114:Liver Neoplasms, Experimental / Q000503:physiopathology*; D008263:Macrophage Migration-Inhibitory Factors / Q000302:isolation & purification*; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains
D008263:Macrophage Migration-Inhibitory Factors; D005643:Fucose