Improvement of LIF release by mononuclear cell cultures by 24 h incubation before stimulation with ConA.
By measuring granulocyte migration from clotted plasma droplets placed in Microtest II plates, leucocyte migration inhibitory factor (LIF) production by concanavalin A (ConA) stimulated mononuclear cell (MNC) cultures was tested. Moderate LIF production was observed when mononuclear cells were pulsed with ConA at 0 h, as compared with cells stimulated after 24 h culture which produced a significantly increased amount of LIF. Fresh adherent cells, when added to 24 h incubated MNC cultures before ConA stimulation, decreased LIF production, in contrast to in vitro irradiated or overnight cultured adherent cells. Indomethacin, when present during exposure to a ConA pulse of either MNC or of adherent cell-rich 24 h incubated MNC cultures, improved LIF activity significantly. This suggests that prostaglandin producing adherent cells, presumably cooperating with radiosensitive short-lived immunoregulatory T cells, are involved in suppression of LIF production by MNC cultures stimulated by ConA at 0 h.
D002448:Cell Adhesion; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis* / Q000378:metabolism; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis*; D009000:Monocytes / Q000276:immunology; D013997:Time Factors
D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D003208:Concanavalin A; D007213:Indomethacin