Genetic effects of some platinum co-ordination complexes on E.coli DNA as revealed by transformation studies.
E. coli chromosomal DNA was treated with various Pt co-ordination compounds and then used as donor DNA in E. coli transformation. Genetic analysis of transformants obtained with Pt-treated DNA showed effects of cis-diamminedichloroplatinum(II) (cis-Pt(II)) and cis-Pt-dimethyl-1,3-diaminopropane Cl4 (cis-Pt(IV) (DMDAP)) on the processing of the DNA. With trans-diamminedichloroplatinum(II) (trans-Pt(II)) applied in similar concentrations no effects were found. The effects of cis-Pt(II) and cis-Pt(IV) (DMDAP) on the genetic processing were different. The effects of cis-Pt(II) could be explained by assuming intra-strand crosslinks as an important lesion.
D002945:Cisplatin / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D007536:Isomerism; D009944:Organoplatinum Compounds / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014170:Transformation, Genetic / Q000187:drug effects*
D003432:Cross-Linking Reagents; D004269:DNA, Bacterial; D009944:Organoplatinum Compounds; C034697:transplatin; C035246:tetrachloro(2,2-dimethyl-1,3-propanediamine-N,N')platinum; D002945:Cisplatin