[Horseradish peroxidase: kinetic studies and optimization of the activity determination with the substrates H2O2 and o-phenylenediamine (author's transl)].
To optimize the determination of the activity of peroxidase, an enzyme often used as a marker and indicator system in enzyme immunoassays, the kinetics of the catalytic reaction and its dependence on the H2O2 and o-phenylenediamine concentration at various pH values were investigated. In addition, the influence of buffer systems, the reaction temperature, and the reaction time were determined. Based on the results, a test procedure is proposed for the measurement of peroxidase down to concentrations of 2 ng/l.
D006735:Horseradish Peroxidase / Q000378:metabolism*; D006861:Hydrogen Peroxide; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D007700:Kinetics; D010544:Peroxidases / Q000378:metabolism*; D010655:Phenylenediamines; D013696:Temperature
D010655:Phenylenediamines; C034193:1,2-diaminobenzene; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D010544:Peroxidases
Peroxidase aus Meerrettich: Kinetische Studien und Optimierung der Aktivitätsbestimmungh mit den Substraten H2O2 und o-Phenylendiamin.