An enzyme immunoassay for serum tobramycin.
A heterogeneous enzyme immunoassay for serum tobramycin was developed using tobramycin-beta-D-galactosidase conjugate as the labelled antigen and 4-methylumbelliferyl-beta-D-galactoside as the fluorescence substrate. B/F separation was carried out using an immobilized second antibody, Immunobead. The minimal detectable level was 10 pg/tube or 0.2 microgram/ml of serum. Assay recovery was excellent and the intra-assay and inter-assay variances were 4.41% and 12.6%, respectively. The values from the enzyme immunoassay agreed well with those from radioimmunoassay and bioassay. This assay system is highly sensitive and can be easily applied to other drugs and hormones.
D000900:Anti-Bacterial Agents / Q000097:blood*; D000918:Antibody Specificity; D003429:Cross Reactions; D006801:Humans; D007124:Immunoenzyme Techniques; D014031:Tobramycin / Q000097:blood* / Q000276:immunology
D000900:Anti-Bacterial Agents; D014031:Tobramycin