Simple and convenient method for culturing anaerobic bacteria.
A simple and convenient method for culturing anaerobic bacteria is described. Cultures can be grown in commercially available flasks normally used for preparation of sterile external solutions. A special disposable rubber flask closure maintains anaerobic conditions in the flask after autoclaving. Growth of a variety of anaerobic oral bacteria was comparable to that obtained after anaerobic incubation of broth cultures in Brewer Anaerobic Jars.
D000190:Actinomyces / Q000254:growth & development; D000693:Anaerobiosis; D001419:Bacteria / Q000254:growth & development*; D001431:Bacteriological Techniques / Q000295:instrumentation; D001439:Bacteroides / Q000254:growth & development