Use of an enzyme-linked immunosorbent assay to measure antibody responses in turkeys against Pasteurella multocida.
AN enzyme-linked immunosorbent assay (ELISA) and a microtiter agglutination (MA) test were used in a comparative study to measure the humoral antibody responses of turkeys receiving oral vaccination against fowl cholera. The ELISA was sensitive and measured antibody titers as high as 1:4,409, whereas the highest titers the MA test measured were 1:128. A close correlation was seen between ELISA antibody titers and protection against oral challenge with virulent Pasteurella multocida, whereas a poor correlation was seen between antibody titers measured by MA tests and protection. ELISA substrate reactions from a single serum dilution, measured with a spectrophotometer, could be converted directly from absorbance to antibody titers using a linear regression plot.
D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D001428:Bacterial Vaccines / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D010325:Pasteurella / Q000276:immunology*; D010326:Pasteurella Infections / Q000517:prevention & control / Q000662:veterinary; D011201:Poultry Diseases / Q000517:prevention & control; D014422:Turkeys / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines