Lysis of cell nuclei on perforated electron microscopic supports.
The technique of gentle nuclear lysis for electron microscopic observation is suggested, which comprises adsorption of isolated nuclei on a positively charged pricked supporting film and sequential short-timed installation of the support with its back onto the filter paper moistened by lysing solution. The advantage of the method is slow, gradient-like, and to some extent regulated treatment allowing to visualize nuclear structures in their integrity.
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D003584:Cytological Techniques; D008854:Microscopy, Electron / Q000379:methods*; D051381:Rats; D013950:Thymus Gland / Q000648:ultrastructure