Self-resistance of a Streptomyces which produces istamycins.
Streptomyces tenjimariensis SS-939, a producer of istamycins, is highly resistant to its own antibiotics and grows in Tryptic Soy Broth containing istamycin A or B at 3,000 microgram/ml. No istamycin-inactivating enzyme was detected in extracts of strain SS-939. Polyphenylalanine synthesis in an in vitro system, consisting of the S-150 fraction and ribosomes prepared from strain SS-939, was not inhibited by 200 microgram/ml of istamycins. Using reciprocally reconstituted systems consisting of S-150 fractions and ribosomes from strain SS-939 and those from Streptomyces griseus ISP5236 (istamycin-sensitive strain), ribosomes of strain SS-939 were found to be resistant to istamycins. Thus, ribosomes have the main role in the self-resistance mechanism of S. tenjimariensis SS-939.
D000617:Aminoglycosides / Q000096:biosynthesis / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000378:metabolism; D010452:Peptide Biosynthesis; D010455:Peptides; D012270:Ribosomes / Q000378:metabolism; D013302:Streptomyces / Q000187:drug effects* / Q000378:metabolism
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D010455:Peptides; C012001:polyphenylalanine; C022949:istamycins