Neurotoxin-induced hydrolase activity in peripheral nerve.
The effect of neurotoxins which block axonal transport or impulse transmission was examined on sciatic nerve hydrolase activity. Subepineural injection of batrachotoxin (BTX) or colchicine resulted in a 4-9 fold increase in acid protease and N-acetylglucosaminidase activity with a smaller increase in acid phosphatase activity. The enzyme remained elevated for up to 48 days. Similar injections of tetrodotoxin (TTX) led to a relatively small increase in the activity of these enzymes which returned to normal values within two days. The prolonged increase in hydrolase activity produced by BTX and colchicine suggests that blockade of axonal transport may be mediated by nerve necrosis.
D000118:Acetylglucosaminidase / Q000378:metabolism*; D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D016282:Aspartic Acid Endopeptidases; D001496:Batrachotoxins / Q000494:pharmacology*; D010450:Endopeptidases / Q000378:metabolism*; D006596:Hexosaminidases / Q000378:metabolism*; D007700:Kinetics; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051379:Mice; D009498:Neurotoxins / Q000494:pharmacology; D012584:Sciatic Nerve / Q000187:drug effects / Q000201:enzymology*; D013779:Tetrodotoxin / Q000494:pharmacology*
D001496:Batrachotoxins; D009498:Neurotoxins; D013779:Tetrodotoxin; D000135:Acid Phosphatase; D006596:Hexosaminidases; D000118:Acetylglucosaminidase; D010450:Endopeptidases; D016282:Aspartic Acid Endopeptidases