[Studies on degradation of cartilage proteoglycan by rheumatoid synovial tissue. Part II: On the property of acid and neutral proteases obtained from rheumatoid synovial tissue (author's transl)].
1. Proteolytic enzymes are likely to play the main role on the proteoglycan (PG)-degrading activity of rheumatoid synovium. In this paper, the presence of cathepsin D, cathepsin B, lysosomal elastase and cathepsin G in rheumatoid synovium is established by isolation, purification, and characterization of these proteases. 2. The degradation of MgCl2-extracted PG from bovine nasal cartilage was performed by using these proteases and the property of the products was studied by the viscosity, Sepharose CL-4B chromatography, Agarose/polyacrylamide-gel electrophoresis, hexosamine analysis and amino acids analysis. 3. These proteases reduced the viscosity of PG solutions and the reaction was inhibited by addition of pepstatin, antipain, elastatinal and chymostatin for each protease. 4. The size and chemical composition of the degradation products varied with the different proteases. Of the four proteases, cathepsin G produced the largest glycosaminoglycan multi-chain peptides and cathepsin B produced the smallest contained chondroitin single-chain peptide. Each protease specifically split PG core protein and the degradation products particularly indicated the characteristic structure of core peptides. 5. The results suggest that these proteases may be contributed to the breakdown of cartilage PG in rheumatoid arthritis.
D000596:Amino Acids / Q000032:analysis; D001172:Arthritis, Rheumatoid / Q000201:enzymology*; D002401:Cathepsin B; D002402:Cathepsin D; D056649:Cathepsin G; D002403:Cathepsins / Q000032:analysis; D005260:Female; D006801:Humans; D008875:Middle Aged; D010196:Pancreatic Elastase / Q000032:analysis; D010447:Peptide Hydrolases / Q000032:analysis*; D011509:Proteoglycans / Q000378:metabolism; D012697:Serine Endopeptidases; D013583:Synovial Membrane / Q000201:enzymology*
D000596:Amino Acids; D011509:Proteoglycans; D002403:Cathepsins; D010447:Peptide Hydrolases; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G; D010196:Pancreatic Elastase; D002401:Cathepsin B; D002402:Cathepsin D