[The SPHA technic (solid phase hemadsorption) in syphilis serology. A year's experience in routine laboratory procedure].
The first results in routine syphilis serology with the new solid-phase hemadsorption assay (SPHA) are presented. Sera (63019) were screened by the VDRL test and by the automated hemagglutination assay with Treponema pallidum antigen (AMHA-TP). Samples reactive to one or both of these tests were further examined by the FTA-ABS test, the IgM-FTA-ABS test, the 19S IgM-FTA-ABS test (IgM-FTA-ABS test performed with the 19S fraction of the serum after gelfiltration) and the IgM-SPHA test. IgM-SPHA reactive sera derived from patients who were in the early stage or in the phase of latency (of undefinable duration), further from patients with active neurosyphilis and from 26 patients after reinfection. Erroneous results in the IgM-FTA-ABS test caused by a competitive inhibition of IgM by IgG can be eliminated by the IgM-SPHA method. The agreement between 19S IgM-FTA-ABS test and IgM-SPHA test is above 96.3%. As compared to the fluorescence test, the IgM-SPHA test is simpler to perform and can be easily automated.
D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000032:analysis*; D005260:Female; D005455:Fluorescent Antibody Technique; D006382:Hemadsorption; D006386:Hemagglutination Tests; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009494:Neurosyphilis / Q000175:diagnosis; D013588:Syphilis Serodiagnosis / Q000379:methods*; D014210:Treponema pallidum / Q000276:immunology*
D000907:Antibodies, Bacterial; D007075:Immunoglobulin M
Die SPHA-Technik (Solid-Phase-Hämadsorption) in der Syphilisserologie. Einjährige Erfahrung im Routinebetries.