A simple and inexpensive method for 3-dimensional visualization of neurons reconstructed from serial sections.
Neurons stained by the intracellular injection of horseradish peroxidase or fluorescent dyes are often reconstructed from serial sections as a projection onto a 2-dimensional plane, with consequent loss of depth information. We have developed a simple, rapid and inexpensive method that retains depth information and permits visualization of neuronal structure in 3 dimensions, either as stereo pairs of red-green anaglyph drawings. The procedure does not require computer assistance and can be accomplished simply with pencil and paper, making it suitable for routine laboratory use. The small effort required in drawing stereo reconstructions is more than compensated for by the enhanced accuracy and understanding or morphology provided.
D000818:Animals; D005456:Fluorescent Dyes; D006652:Histological Techniques; D006735:Horseradish Peroxidase; D007395:Interneurons / Q000166:cytology; D009474:Neurons / Q000166:cytology*
D005456:Fluorescent Dyes; D006735:Horseradish Peroxidase