Characterization of B-cell and monocyte antibodies in recipient sera and in eluates from rejected kidney allografts.
The specificity of B-cell antibodies developing in kidney transplant recipients was investigated by blocking with monoclonal anti-DR and chicken anti-human IA antibody. Among 34 recipients who ultimately rejected their kidney allograft, 35% displayed antibodies against B cells before transplantation, most of which were blocked by the anti-DR reagents. Thirty-three of 34 recipients developed B-cell lymphocytotoxins after nephrectomy, and over half of these were not blocked by monoclonal anti-DR. B-cell antibodies were found also in the majority of the kidney eluates, and most of them were shown to be directed against HLA-DR antigens, Monocyte-specific antibodies, which were not blocked by chicken anti-human IA serum, were frequently found in eluates from kidney allografts undergoing rejection. The methods described appear to be useful for the characterization of B-cell and monocyte antibodies developing in kidney transplant recipients. The possibility of correlation with allograft outcome is currently being investigated.
D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D006084:Graft Rejection; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007518:Isoantibodies / Q000302:isolation & purification*; D007668:Kidney / Q000276:immunology*; D016030:Kidney Transplantation; D009000:Monocytes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D006680:HLA Antigens; D007518:Isoantibodies