Surface expression of a nonstructural antigen on influenza A virus-infected cells.
Rabbit antiserum to the influenza A virus nonstructural protein, NS1, was used for indirect immunofluorescence studies of infected cells. Nonstructural antigens were detected on surfaces of P815 cells as early as 4 h after infection with A/WSN/40 virus. Adsorption of the serum with virion structural proteins did not affect the observed fluorescence, and a progressive increase in surface fluorescence at later times postinfection indicated that the surface antigen was newly synthesized during the replication cycle.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D009980:Influenza A virus / Q000276:immunology*; D051379:Mice; D008970:Molecular Weight; D009976:Orthomyxoviridae Infections / Q000276:immunology*
D000956:Antigens, Viral