Enzyme immunoassay for tartrate-resistant acid phosphatase.
An immunochemical method for quantitative analysis of the tartrate-resistant acid phosphatase (EC 3.1.3.2), band 5, is presented. This method involves precipitation of the enzyme from the serum by the antibody specific to band 5 and by sheep anti-rabbit immunoglobulin, followed by analysis of the enzyme activity in the precipitate. The precipitation procedure eliminates the interferences of the tartrate-sensitive phosphatase of all tissues, of the tartrate-resistant phosphatase of erythrocytes, and of unknown substances that interfere with the colorimetric method. We compare the present method with previously described colorimetric and electrophoretic methods.
D000135:Acid Phosphatase / Q000097:blood*; D002852:Chromatography, Ion Exchange; D003124:Colorimetry; D004586:Electrophoresis; D006801:Humans; D007124:Immunoenzyme Techniques; D013644:Tartrates / Q000494:pharmacology*
D013644:Tartrates; D000135:Acid Phosphatase