Limitations of Gullino-type chambers for collection of interstitial proteins from human tumour xenografts: a histological and theoretical study.
GULLINO-type diffusion chambers, made entirely from polytetrafluoroethylene (P.T.F.E.) were implanted subcutaneously into athymic nude mice either alone or with tumour mince from a human colonic tumour xenograft. Those implanted with mince became incorporated into the resulting tumours. Chambers were removed at various times following implantation and histological sections prepared from surrounding tissues. A consistent histological picture was seen adjacent to all parts of the implants comprised of a foreign body giant cell reaction with fibrosis, irrespective of the presence of tumour. We argue that in the absence of any reaction, such chambers may collect specific proteins and allow estimation of total interstitial protein content, but are unsuitable for determining the effective in vivo concentration of any particular interstitial protein. It is further suggested that the presence of reactive tissue around the chambers could introduce further sources of error, adequate controls for which are essential for interpretation of data relating of interstitial proteins collected in this manner.
D000818:Animals; D003110:Colonic Neoplasms / Q000473:pathology; D005110:Extracellular Space / Q000032:analysis*; D005549:Foreign-Body Reaction / Q000473:pathology; D006801:Humans; D008297:Male; D008722:Methods; D051379:Mice; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000302:isolation & purification*; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000032:analysis*; D014183:Transplantation, Heterologous
D009363:Neoplasm Proteins