The effects of orthovanadate on fatty acid synthesis in isolated rat hepatocytes.
Extracellular Ca2+ stimulated fatty acid synthesis in isolated rat hepatocytes. Orthovanadate (0.2--2.0 mM), an inhibitor of Ca2+-dependent ATPases, stimulated fatty acid synthesis in both the presence and the absence of extracellular Ca2+. Insulin stimulated fatty acid synthesis only in the presence of extracellular Ca2+. The contribution of extracellular Ca2+ to insulin stimulation of fatty acid synthesis is discussed.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D005227:Fatty Acids / Q000096:biosynthesis*; D005260:Female; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology*
D005227:Fatty Acids; D007328:Insulin; D014639:Vanadium; D014638:Vanadates; D002118:Calcium