The properties of the tRNA . protein complex of the Escherichia coli ribosome. Interaction with tRNA, 5-S RNA and 30-S ribosomal subunit.
A complex between immobilized tRNA and Escherichia coli 50-S ribosomal subunit proteins provides binding sites for several other components of the protein biosynthesis system. We found that this complex binds 5-S RNA. tRNA and 30-S ribosomal subunits. The binding of 5-S RNA and tRNA takes place at equilibrium; in 10 mM Tris/HCl buffer pH 7.5, containing 10 mM MgCl2 and 100 mM KCl at 4 degrees C it is characterized by the dissociation constants 1.7 X 10(-7) M and 4 X 10(-7) M respectively. We show that the performed complex between tRNA and 50-S ribosomal subunit protein can bind these two RNAs simultaneously by forming a subribosomal domain consisting of two molecules of tRNA, 5-S RNA and the ribosomal proteins L2, L15, L16, L17, L18, L22, L33 and L34. The preformed tRNA . protein complex interacts also with the 30-S ribosomal subunit, suggesting that is located at the ribosomal interface.
D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D012335:RNA, Ribosomal / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013045:Species Specificity
D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D012343:RNA, Transfer