Solubilization and reconstitution of the melibiose carrier from a plasmid-carrying strain of Escherichia coli.
A strain of EScherichia coli was constructed containing a plasmid from the Clarke-Carbon collection that showed high levels of melibiose transport activity. Membranes from the plasmid-containing strain were extracted with octyl-beta-D-glucopyranoside and melibiose transport was reconstituted in liposomes. The proteoliposomes exhibited counterflow activity, as well as membrane potential and sodium gradient-driven melibiose accumulation.
D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D007700:Kinetics; D008553:Melibiose / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010957:Plasmids; D012995:Solubility; D013045:Species Specificity; D027981:Symporters
D026901:Membrane Transport Proteins; D027981:Symporters; C021548:melibiose permease; D008553:Melibiose