An NADP-linked prostacyclin dehydrogenase in rabbit kidney.
An NADP-linked 15-hydroxyprostaglandin dehydrogenase specific for prostacyclin was purified 1,300-fold from rabbit kidney. Prostaglandins E2, F2 alpha, and 6-keto PGF1 alpha and thromboxane B2 were oxidized by the purified enzyme with rates of reaction less than 4% that of PGI2. Unlike other rabbit kidney NADP-linked 15-hydroxyprostaglandin dehydrogenases, this enzyme catalyzes oxido-reduction more rapidly at the 15- position than at the 9- position and does not utilize NAD as a cofactor. It has a molecular weight of 62,000 and migrates on polyacrylamide disc gel electrophoresis as a single diffuse band. The reaction product was identified by thin-layer chromatography as 6,15-diketo PGF1 alpha. Prostacyclin dehydrogenase is the first 15-hydroxyprostaglandin dehydrogenase described which is specific for the metabolism of prostacyclin.
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D015237:Dinoprost; D015232:Dinoprostone; D011464:Epoprostenol / Q000378:metabolism*; D006911:Hydroxyprostaglandin Dehydrogenases / Q000302:isolation & purification / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D009249:NADP / Q000494:pharmacology; D011453:Prostaglandins / Q000378:metabolism*; D011458:Prostaglandins E / Q000378:metabolism; D011460:Prostaglandins F / Q000378:metabolism; D011817:Rabbits; D013379:Substrate Specificity
D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D009249:NADP; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost; D011464:Epoprostenol; D006911:Hydroxyprostaglandin Dehydrogenases; D015232:Dinoprostone