Colicin Ib does not cause plasmid-promoted abortive phage infection of Escherichia coli K-12.
A 2.2 kilobase (kb) fragment of ColIdrd-1 cloned in pBR325 causes phage T5 and BF23 infections of Escherichia coli K-12 to abort. This abortive infection is associated with leakage of beta-galactosidase from the cell. A recombinant plasmid containing a 2.8 kb fragment of ColIdrd-1 encodes colicin Ib but fails to cause abortive infection. Tn5 and Tn10 insertions into ColIdrd-1 that abolish colicin Ib production have no effect on the abortive infection phenotype. These findings are inconsistent with a previously proposed role for colicin Ib in causing phage infections to abort.
D003086:Bacteriocin Plasmids; D003087:Colicins / Q000235:genetics*; D003090:Coliphages / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D010957:Plasmids; D014779:Virus Replication
D003087:Colicins; D004269:DNA, Bacterial; D004274:DNA, Recombinant