[An ELISA study of the intestinal and serum anti-LPS antibody response in BALB/c mice fed with various mutants of "Salmonella typhi-murium" (author's transl)].
The specific anti-LPS serum antibody response in BALB/c mice, before and after an oral booster with different non-pathogenic strains of Salmonella typhi-murium, was measured by an ELISA technique. Serum response to LPS was found to depend on the length of the LPS polysaccharide moiety in "rough" mutants. The M206 "smooth" mutant induced a less marked anti-LPS response. The immunogenicity of the polysaccharidic "core" appears to be modified by the O antigen. The specific antibody response in the gut only becomes marked following after booster of Ra, Rc and M206 mutants.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D007422:Intestines / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D012486:Salmonella typhimurium / Q000276:immunology*; D014611:Vaccination
D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides
Etude, par la méthode ELISA, du titre des anticorps sériques et intestinaux anti-LPS chez la souris BALB/c vaccinée à l'aide de mutants "rough" et "smooth" de Salmonella typhi-murium.