Enzyme-linked immunosorbent assay for measurement of anti-Moraxella bovis antibodies.
Antibodies of the immunoglobulin (Ig) G and IgA classes specific for Moraxella bovis in serum or lacrimal secretions of cattle were detected with an enzyme-linked immunosorbent assay, using soluble extracts of M bovis or whole organisms. The amount of detectable antibodies was influenced by the type of antigenic preparation used in the enzyme-linked immunosorbent assay. The test indicated that 2 subcutaneous inoculations of M bovis cells induce significantly (P less than 0.0001) increased anti-M bovis serum antibodies. The infection of calves with M bovis induced specific IgG antibodies in serum and predominantly IgA antibodies in lacrimal secretions.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D001424:Bacterial Infections / Q000175:diagnosis / Q000662:veterinary; D001428:Bacterial Vaccines / Q000276:immunology; D002417:Cattle / Q000276:immunology*; D002418:Cattle Diseases / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007637:Keratoconjunctivitis / Q000175:diagnosis / Q000662:veterinary; D009016:Moraxella / Q000276:immunology*; D013666:Tears / Q000276:immunology; D014611:Vaccination / Q000662:veterinary
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007070:Immunoglobulin A; D007074:Immunoglobulin G