Primary isolation of Campylobacter fetus subspecies jejuni.
Primary isolation of Campylobacter fetus ss. jejuni following selective filtration can be achieved equally well on a Columbia agar supplemented with sheep blood (5%), horse blood (5-10%), or heat-treated horse blood (chocolate agar). Larger, but not more, colonies were formed at 42C. However, the higher temperature inhibits C. fetus ss. Intestinalis. Reduced oxygen tension (5%) was advantageous for cultures on media supplemented with sheep blood. All other media tested did equally well as long as the atmosphere was enriched with 5-10% CO2.
D000818:Animals; D001431:Bacteriological Techniques; D001769:Blood; D002167:Campylobacter / Q000302:isolation & purification*; D002168:Campylobacter fetus / Q000302:isolation & purification*; D003470:Culture Media; D003967:Diarrhea / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005374:Filtration; D006736:Horses; D006801:Humans; D011200:Poultry; D012756:Sheep
D003470:Culture Media