Effects of various prostanoids on the in vitro metabolism of bovine articular chondrocytes.
The dose-dependent effects of 9 prostanoids (PGA1, PGA2, PGE1, PGE2, PGF1 alpha, PGF2 alpha, PGD2, PGI2, 6 keto-PGF1 alpha) on metabolism of cultured bovine articular chondrocytes were investigated. Most prostanoids dose-dependently inhibited 35SO4= and 3H-glycine incorporation. At 25 microgram/ml, the inhibitory sequence was A2 greater than or equal to D2 greater than E2= E1 = A1 greater than 6 keto-F1 alpha greater than F1 greater than F2, but sensitivity (lowest dose eliciting inhibition) followed the sequence E2 greater than 6 keto-F1 alpha = F1 greater than A2 = D2 greater than E1 greater than A1. At 25 microgram/ml, PGA2 also inhibited incorporation of 3H-cytidine and 3H-thymidine, but had no significant effect on 3H-glucose or 14C-xylose incorporation. The inhibitory effect of PGA2 was apparent after 30 minutes exposure for 35SO4= and after 60 minutes for 3H-cytidine, and was still present up to 72 hours following incubation in fresh non-PG-containing medium. PGI2 had no significant effect on 35SO4= incorporation but at concentrations below 10 microgram/ml enhanced uptake of 3H-glycine. The PG-induced inhibitory effect was apparently not due to cell damage as indicated by measurement of 3H-glucose metabolism and lactate production.
D000527:Alprostadil; D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D015232:Dinoprostone; D011464:Epoprostenol / Q000494:pharmacology; D005998:Glycine / Q000378:metabolism; D011454:Prostaglandins A / Q000494:pharmacology; D011457:Prostaglandins D / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D011465:Prostaglandins, Synthetic / Q000494:pharmacology*; D013431:Sulfates / Q000378:metabolism
D011454:Prostaglandins A; D011457:Prostaglandins D; D011458:Prostaglandins E; D011465:Prostaglandins, Synthetic; D013431:Sulfates; D011464:Epoprostenol; D000527:Alprostadil; C100008:prostaglandin A2; D015232:Dinoprostone; D005998:Glycine; C100573:prostaglandin A1