title: The stimulus-secretion coupling of glucose-induced insulin release. Environmental influences on L-glutamine oxidation in pancreatic islets.
abstract: L-Glutamine at a near-physiological concentration (1.0mM) was rapidly taken up and metabolized in rat pancreatic islets. The rate of glutamine deamidation much exceeded that of glutamate conversion into 2-oxoglutarate, the latter conversion being mediated mainly by transamination reactions. The production of 14CO2 from L-[U-14C]glutamine, which reflected the generation of ATP through the metabolism of exogenous glutamine, appeared to be regulated by the redox state of nicotinamide nucleotides and the ATP content of the islet cells. The influence of environmental factors on glutamine oxidation was examined in order to identify ATP-requiring processes. Glutamine oxidation was decreased in the absence of extracellular Ca2+, under conditions aiming at inhibition of the (Na+ + NA+)-dependent ATPase and, provided that glucose was present in the incubation medium, by cycloheximide. These findings were interpreted to suggest that the handling of Ca2+ by the islet cells, the active transport of univalent cations and the biosynthesis of proinsulin represent three major ATP-consuming processes in this fuel-sensor organ.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism*; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008954:Models, Biological; D010084:Oxidation-Reduction; D051381:Rats
chemical_list: D000963:Antimetabolites; D007328:Insulin; D005973:Glutamine; D000255:Adenosine Triphosphate; D005947:Glucose; D002118:Calcium