title: Characterization of T-cell surface proteins bound by heterologous antisera to antigen-specific T-cell products.
abstract: Heterologous antisera specific for murine T-cell antigen-recognition molecules were prepared by immunization of rabbits with dinitrophenyl-specific murine T-cell suppressor factors that had been purified by hapten-affinity chromatography. The antisera (i) bind to antigen-specific T-cell products that differ in their antigen-recognizing specificity; (ii) absorb the specific suppressor activity in preparations containing suppressor factors; (iii) stain all Lyt2+ T cells brightly in indirect immunofluorescence examination, stain some Lyt1+ cells (with low intensity), and do not stain B cells; (iv) precipitate cell membrane proteins from T cells that bear striking structural resemblance to the antigen-specific molecules used for immunization. These results suggest that, like B cells, there is a commonality between antigen-specific effector molecules released by T cells and their membrane-associated receptors.
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex; D000954:Antigens, Surface / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D008565:Membrane Proteins / Q000032:analysis*; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D011863:Radioimmunoassay; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000936:Antigen-Antibody Complex; D000954:Antigens, Surface; D007106:Immune Sera; D008565:Membrane Proteins