Non-random binding of N-acetoxy-N-2-acetylamino-fluorene to chromatin subunits as visualized by immunoelectron microscopy.
The influence of chromatin structure on the accessibility of DNA to the model ultimate carcinogen N-acetoxy-N-2-acetylaminofluorene (N-Aco-AAF) was investigated by means of an immunoelectron microscopic technique developed recently. An homogeneous population of core particles or trinucleosomes from chicken erythrocytes, was submitted to electrophilic attack by N-Aco-AAF. After DNA isolation, N-2-acetylaminofluorene (AAF) binding sites were mapped upon the DNA fragments using specific antibodies as a probe. Our results indicate a non-random binding of AAF along the DNA. Our data support the results of previous studies showing a preferential binding on the linker region.
D015073:2-Acetylaminofluorene / Q000031:analogs & derivatives*; D000099:Acetoxyacetylaminofluorene / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002645:Chickens; D002843:Chromatin / Q000378:metabolism*; D004912:Erythrocytes / Q000032:analysis; D007158:Immunologic Techniques; D046911:Macromolecular Substances; D008854:Microscopy, Electron / Q000379:methods
D002843:Chromatin; D046911:Macromolecular Substances; D000099:Acetoxyacetylaminofluorene; D015073:2-Acetylaminofluorene