Functional studies in 6 days successful preserved canine kidneys.
In 2 groups of 6 dogs each the right kidney was removed, flushed with Collins and then perfused in a Gambro preservation machine for 6 days. After this period the kidneys were reimplanted in the neck of the subsequently nephrectomized donor. The only difference in Group 2 was that halfway the 6-day hypothermic perfusion the kidneys were taken from the machine and perfused ex vivo for 3 hours by connections with the femoral vessels of the same donor dog. After implantation, only 1 dog from group 1 survived. All 6 dogs in group 2 survived. A low filtration fraction (less than 0.26) after implantation, measured 1 hour after implantation, correlated well with and predicted life sustaining kidney function. During the ex vivo perfusion in group 2, an improving proximal tubular secretion and water reabsorption was observed.
D000818:Animals; D001769:Blood; D001783:Blood Flow Velocity; D003404:Creatinine / Q000097:blood; D004285:Dogs; D005919:Glomerular Filtration Rate; D007668:Kidney / Q000098:blood supply; D016030:Kidney Transplantation; D009926:Organ Preservation; D010477:Perfusion; D013997:Time Factors; D014021:Tissue Preservation; D014462:Ultrafiltration
D003404:Creatinine