[Use of continuous human lymphoblastoid cell lines (T- and B-origin) to produce persistent tick-borne encephalitis virus and Venezuelan equine encephalomyelitis virus infections].
Persistent infection with tick-borne encephalitis virus (TBE) was established in experimentally infected continuous lymphoblastoid human cell lines Raji, L-101 (of B-origin) and 1387 (T-origin) and with Venezuelan equine encephalomyelitis (VEE) virus in Raji and 1387 lines. The persistently infected lines produced infectious virus, the cells showed specific fluorescence in immunofluorescent tests, and electron microscopic examinations revealed TBE and VEE virions in sections.
D001402:B-Lymphocytes; D002460:Cell Line; D002908:Chronic Disease; D004666:Encephalitis Virus, Venezuelan Equine; D004669:Encephalitis Viruses, Tick-Borne; D004675:Encephalitis, Tick-Borne / Q000382:microbiology; D004685:Encephalomyelitis, Venezuelan Equine / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008854:Microscopy, Electron; D013601:T-Lymphocytes
Ispol'zovanie perevivaemykh limfoblastoidnykh liniĭ kletok cheloveka (T- i B-proiskhozhdeniia) dlia polucheniia persistentnoĭ infektsii virusa kleshchegogo éntsefalita i virusa venesuél'skogo éntsefalomielita loshadeĭ.