A method for recovering viruses from sludges.
Primary, activated, and anaerobic mesophilically digested sludges were salted with MgCl2 (divalent cations) or AlCl3 (trivalent cations) and acidified to bind indigenous unadsorbed virions to the sludge solids; the sludges were centrifuged, and the adsorbed virions were eluted from the solids with buffered 10% beef extract. The elution yields with this procedure were superior to those obtained from sludges that had been salted or acidified only. Homogenization of sludges prior to other treatment did not increase the numbers of virions recovered.
D000535:Aluminum; D000077410:Aluminum Chloride; D017607:Aluminum Compounds; D000818:Animals; D002460:Cell Line; D002498:Centrifugation; D002712:Chlorides; D002522:Chlorocebus aethiops; D007668:Kidney; D008274:Magnesium; D015636:Magnesium Chloride; D008828:Microbiological Techniques; D012722:Sewage / Q000032:analysis*; D010948:Viral Plaque Assay; D014780:Viruses / Q000302:isolation & purification*
D017607:Aluminum Compounds; D002712:Chlorides; D012722:Sewage; D015636:Magnesium Chloride; D000077410:Aluminum Chloride; D000535:Aluminum; D008274:Magnesium