Inhibition of L-cell growth in agarose (ILGA): a simple inexpensive method for the detection and quantitation of factors inhibiting tumor cell growth.
By taking advantage of the ability of L-929 tumor cells to grow in agarose, we have developed an in vitro method for detecting tumor necrosis factor (TNF), a potent inhibitor of tumor cell growth. When placed in a well cut in agarose containing L-cells, TNF inhibits L-cell growth in the area adjacent to the well. The size of this area of growth inhibition is proportional to the amount of TNF placed in the well. The size of this area of growth inhibition is proportional to the amount of TNF placed in the well. This assay can be used to monitor TNF activity during purification procedures and may also prove useful in the analysis of other factors interfering with tumor cell proliferation.
D000818:Animals; D002852:Chromatography, Ion Exchange; D006023:Glycoproteins / Q000032:analysis* / Q000494:pharmacology; D006131:Growth Inhibitors / Q000032:analysis* / Q000494:pharmacology; D007158:Immunologic Techniques; D007739:L Cells / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D013936:Thymidine / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha
D006023:Glycoproteins; D006131:Growth Inhibitors; D014409:Tumor Necrosis Factor-alpha; D013936:Thymidine