[Ability of isoelectric focusing to separate antitetanus antibodies in venous blood].
In a previous study, we have showed a great heterogeneity of antitetanus antibodies using polyacrylamide thin layer isoelectrofocusing. In this study, we have improved the resolution of the focused antitetanus antibodies by electrofocusing in sucrose density gradients, which is a suitable procedure for preparative work. After isoelectrofocusing the elution is performed according to any pH variation. For each fraction, we have determined pH, absorbance at 280 nm, protein level by method of Lowry, IgG concentration using immunonephelometric method and antitetanus antibodies concentration by radioimmunoassay. Furthermore, the purity of each fraction is verified by polyacrylamide slab gel isoelectrofocusing. It is concluded that the isoelectric point of antitetanus antibodies is alkaline range between 7.5 and 8.5. This precise knowledge could afford an opportunity for the industrial preparation of antitetanus antibodies.
D000042:Absorption; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D001798:Blood Proteins; D002499:Centrifugation, Density Gradient; D003017:Clostridium tetani / Q000276:immunology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D011863:Radioimmunoassay; D013742:Tetanus / Q000276:immunology*; D014680:Veins / Q000276:immunology
D000907:Antibodies, Bacterial; D001798:Blood Proteins; D007074:Immunoglobulin G
Pouvoir discriminant de l'isoélectrofocalisation en veine liquide pour la séparation des immunoglobulines antitétaniques.