Stability of prostacyclin in human plasma and whole blood: studies on the protective effect of albumin.
The biological half-life of prostacyclin in Krebs solution, human cell-free plasma or whole blood was measured by bracket assay on ADP-induced platelet aggregation. At 37 degrees C, pH 7.4, plasma and blood reduced the rate of loss of antiaggregatory activity compared with Krebs solution. The protective effect of plasma was greater than that of whole blood. This effect could be partially mimicked by the addition of human or bovine serum albumin to the Krebs solution. The stabilisation afforded by human serum albumin was dependent on the fatty acid content of the albumin, although this was less important for bovine serum albumin.
D000328:Adult; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D011464:Epoprostenol / Q000097:blood* / Q000494:pharmacology; D005260:Female; D006207:Half-Life; D006801:Humans; D006868:Hydrolysis; D008297:Male; D010949:Plasma; D010974:Platelet Aggregation / Q000187:drug effects*; D011453:Prostaglandins / Q000097:blood*; D012709:Serum Albumin / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D003470:Culture Media; D011453:Prostaglandins; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D011464:Epoprostenol