Localization of a glycosphingolipid, asialo GM1, in rat immunocytes.
One of the TLMA (rat T-lymphocyte-macrophage-associated antigens) was characterized as asialo GM1, Gal(beta, 1-3)GalNAc(beta, 1-4)Gal(beta, 1-1)-Cer by two assay methods using monospecific anti-asialo GM1 antibody, immunocytotoxicity testing and immunostaining. Nylon column-enriched T lymphocytes (non-adherent cells) were highly cytolyzed by anti-asialo GM1 antiserum and complement as compared with adherent cells (non T cells). Forty % of peritoneal exudate macrophages and 20% of granulocytes were also cytolyzed by anti-asialo GM1 antiserum. On the other hand, 5% of thymic cells and 60-80% of lymph node T cells were immunostained by anti-asialo GM1 antibody. These asialo GM1-positive cells in thymus were found to be localized in the cortico-medulla junction. About 20% of macrophages and 2)% of granulocytes were also immunostained by anti-asialo GM1 F (ab')2 fragment. These results strongly support the previous conclusion that TLMA can be identified as asialo GM1. Asialo GM1 was universally expressed on a certain population of macrophages of various tissues, but not on all of the population. Alveolar macrophages were also asialo GM1-positive.
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D005455:Fluorescent Antibody Technique; D005677:G(M1) Ganglioside / Q000378:metabolism*; D005732:Gangliosides / Q000378:metabolism*; D006017:Glycolipids / Q000378:metabolism; D006098:Granulocytes / Q000378:metabolism*; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology
D005732:Gangliosides; D006017:Glycolipids; D005677:G(M1) Ganglioside