Regulation of immune reactivity to collagen in human beings.
Denaturated beef collagen was tested for its ability to induce the production of leukocyte inhibition factor among the peripheral blood mononuclear cells from patients with rheumatoid arthritis and normal individuals. Responsiveness, defined as the production of leukocyte inhibition factor sufficient to cause greater than 20% inhibition of leukocyte migration, was significantly (P less than 0.001, X2 = 31.1) associated with HLA-DR4. All HLA-DR4 positive individuals, including subjects without any evidence of synovitis, were collagen responders. There was no significant (P = 0.3) difference in the absolute reactivity of HLA-DR4+ versus HLA-DR4- individuals to respond to another antigen, Candida albicans. Collagen reactivity required interactions between macrophages and T cells and was directed against determinants inherent in the linear polypeptide, (Gly-Pro)n. In 5 normal HLA-DR4- nonresponders tested, absence of discernable reactivity to collagen was associated with the presence of antigen-specific, radiosensitive suppressive T cells. These studies suggest that during the physiologic metabolism of collagen all individuals are exposed to Gly-Pro determinants normally buried in the interstices of the collagen triple helix. In individuals whose major histocompatibility complex contains genes linked to those coding for HLA-DR4, this results in the activation of reactive T cells. Conversely, in individuals lacking these genes, collagen-specific suppressive cells predominate.
D000818:Animals; D000946:Antigens, Fungal; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D002176:Candida albicans / Q000276:immunology; D002417:Cattle; D003094:Collagen / Q000494:pharmacology*; D000949:Histocompatibility Antigens Class II; D006801:Humans; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis*; D008214:Lymphocytes / Q000528:radiation effects; D008222:Lymphokines / Q000096:biosynthesis*; D010455:Peptides / Q000494:pharmacology; D011509:Proteoglycans / Q000494:pharmacology
D000946:Antigens, Fungal; D000949:Histocompatibility Antigens Class II; D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D010455:Peptides; D011509:Proteoglycans; D003094:Collagen