Differential distribution in rat basal ganglia of Met-enkephalin- and Met-enkephalin Arg6 Phe7-like peptides revealed by immunohistochemistry.
Antisera to Met-enkephalin and its heptapeptide variant Met-enkephalin Arg6Phe7 were used in immunohistochemistry to study distribution of immunoreactive material in rat basal ganglia. In the pallidum, the Met-enkephalin antiserum, but not the Met-enkephalin Arg6Phe7 antiserum, revealed abundant nerve terminals, whereas in the caudate-putamen the heptapeptide antiserum demonstrated numerous immunoreactive cell bodies that were not shown by the Met-enkephalin antiserum. The differential distribution of immunoreactivity is compatible with distinct physiological roles for the two peptides in basal ganglia.
D000818:Animals; D001479:Basal Ganglia / Q000378:metabolism*; D002421:Caudate Nucleus / Q000378:metabolism; D004723:Endorphins / Q000378:metabolism*; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives*; D004745:Enkephalins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005917:Globus Pallidus / Q000378:metabolism; D006651:Histocytochemistry; D008297:Male; D010455:Peptides / Q000378:metabolism*; D011699:Putamen / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D004723:Endorphins; D004745:Enkephalins; D010455:Peptides; D004744:Enkephalin, Methionine; C027511:enkephalin-Met, Arg(6)-Phe(7)-; C028718:enkephalin-Met, Lys(6)-