[Role of the microtubules in the organization of the Golgi complex (author's transl)].
Tubulin, clathrin and a protein specific for the Golgi apparatus have been localized in cultures of rat fibroblasts using immunofluorescent techniques. These three antigens are concentrated at a single pole of the cell in close contact with the nucleus. Depolymerization of microtubules induced by colcemid treatment was accompanied by a redistribution of the clathrin and also of the specific Golgi antigen to the cell periphery. During mitosis, clathrin and the Golgi antigen are localized in the area where the microtubules are abundant. The significance of the localization of proteins associated with distinct organelles observed under the aforementioned conditions is discussed.
D000818:Animals; D002966:Clathrin; D005347:Fibroblasts / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006056:Golgi Apparatus / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000032:analysis*; D008870:Microtubules / Q000648:ultrastructure*; D008938:Mitosis; D011506:Proteins / Q000032:analysis; D051381:Rats; D014404:Tubulin / Q000032:analysis*
D002966:Clathrin; D008565:Membrane Proteins; D011506:Proteins; D014404:Tubulin
Rôle des microtubules dans l'organisation du complexe de Golgi.