Substrate specificity of CTP-synthetase from E. coli.
The substrate specificity of CTP-synthetase from E. coli was investigated by means of UTP analogs. This study revealed that the three main structural elements of the UTP molecule were important for the substrate specificity of the enzyme. CTP-synthetase seems to possess an absolute requirement for the beta-D-ribose 5-triphosphate part in UTP. Substitutents in 5-position of UTP, exceeding the size of a tritium atom abolish substrate function.
D019731:Carbon-Nitrogen Ligases; D003570:Cytidine Triphosphate / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D008025:Ligases / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D014500:Uracil Nucleotides; D014544:Uridine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism
D014500:Uracil Nucleotides; D003570:Cytidine Triphosphate; D008025:Ligases; D019731:Carbon-Nitrogen Ligases; C019951:CTP synthetase; D014544:Uridine Triphosphate