title: Biological and biochemical characterization of macrophage activating factor (MAF) in murine lymphocytes: role of mannopyranosyl residue of the MAF molecule in macrophage activation.
abstract: Activation of macrophages with macrophage activating factor (MAF) was evaluated by measuring the intracellular killing activity of murine macrophages against Salmonella typhimurium. Concanavalin A (Con A)-induced MAF-rich fraction was obtained by a Sephadex G-100 column, which contained molecules ranging from 25,000 to 67,000 daltons. The intracellular killing ability of mouse peritoneal macrophages against S. typhimurium was found to be increased by 0.1 M D-mannose as well as by Con A-induced MAF-rich fraction. Both 0.1 M D-mannose and MAF exhibited a similar timing pattern for macrophage activation. The same concentration of D-glucose or L-rhamnose did not change bacterial uptake and intracellular killing by macrophages. Moreover, when MAF-rich fraction was applied to a Con A-Sepharose column, a fraction that was adsorbed on Con A and eluted with 0.1 M alpha-methyl D-mannoside exhibited MAF activity. These results suggest the possibility that mannopyranosyl residues in the MAF molecules play an important role as a ligand in macrophage activation.
mesh_terms: D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D005260:Female; D005947:Glucose / Q000494:pharmacology; D008222:Lymphokines / Q000276:immunology*; D008262:Macrophage Activation; D008297:Male; D008358:Mannose / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis; D012210:Rhamnose / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000276:immunology; D013997:Time Factors
chemical_list: D008222:Lymphokines; D005947:Glucose; D008358:Mannose; D012210:Rhamnose