[Use of stationary-growth broth cultures for obtaining Escherichia enterotoxins. I. Study of the preparations from reference strains of enterotoxigenic Escherichia].
Experiments with standard enterotoxigenic E. coli (ETEC) strains 06:H16, 015:H11, 078:H11, 0148:H28 and 26 nonenterotoxigenic enterobacterial strains used for control were carried out. As a result, the preparation, simple in manufacture and containing thermolabile enterotoxin (TLE) and thermostable enterotoxin (TSE), was obtained: the supernatant fluid (SF) of 48-hour ETEC culture grown at 37 degrees C in flasks with Hottinger's broth without shaking. the possibility of using SF obtained by the centrifugation of the cultures 2 times for 1 hour at 8,000-10,000 r. p. m. was shown. The comparison with the control preparation indicated that the SF thus obtained possessed high biological activity and induced specific reactions in the paw edema test in mice for detecting TLE and in the anal test in suckling mice for detecting TSE.
D000818:Animals; D000833:Animals, Suckling; D001431:Bacteriological Techniques; D003470:Culture Media / Q000378:metabolism; D004768:Enterotoxins / Q000302:isolation & purification* / Q000633:toxicity; D004926:Escherichia coli / Q000254:growth & development*; D051379:Mice; D012015:Reference Standards; D013997:Time Factors
D003470:Culture Media; D004768:Enterotoxins
Primenenie statsionarno vyrashchennykh bul'onnykh kul'tur dlia polucheniia énterotoksinov ésherikhiĭ. Soobshchenie I. Izuchenie preparatov iz étalonnykh shtammov énterotoksigennykh ésherikhii.