Genetic activity of vinylidene chloride in yeast.
Vinylidene chloride (VDC) was tested for its ability to induce both point mutation and mitotic gene conversion in a diploid strain (D7) of the yeast Saccharomyces cerevisiae in a suspension test with and without a mammalian microsomal activation system, and in the intrasanguineous host-mediated assay in  mice. In suspension tests with D7, VCD was toxic but not genetically active without microsomal activation. When a mouse liver 10 000 X g supernatant was included in the suspension tests, dose-related increases in both point mutation and mitotic gene conversion were seen at survival levels greater than 50%, at doses of VCD above 20 mM. In the host-mediated assay, VDC induced both point mutation and mitotic gene conversion when recovered from the liver and kidneys after both acute and sub-acute dosing. Yeasts recovered from the lungs showed little, if any, increase in either point mutation or mitotic gene conversion.
D000818:Animals; D001711:Biotransformation; D004000:Dichloroethylenes / Q000494:pharmacology*; D005785:Gene Conversion / Q000187:drug effects*; D006843:Hydrocarbons, Chlorinated / Q000494:pharmacology*; D051379:Mice; D008938:Mitosis; D009154:Mutation / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics
D004000:Dichloroethylenes; D006843:Hydrocarbons, Chlorinated