[The enzyme linked immuno-sorbent assay (ELISA) method applied for human toxoplasmosis diagnosis (author's transl)].
The enzyme linked immuno-sorbent assay (ELISA) was applied for the diagnosis of toxoplasmosis in man. Examination of different parameters showed that for the same optic density, the concentration of the antigen varies directly with the serum dilution is a linear pattern. Two hundred and thirty eight serum samples were examined by the direct agglutination test, the indirect fluorescent antibody technique and the ELISA method. The ELISA was found to be more sensitive than the other two. Eighty sera were titrated by using the macro method in tubes and the micro method in plates. The latter procedure was more sensitive and showed better discrimination between low and high positives.
D000372:Agglutination Tests; D000906:Antibodies / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis*; D014122:Toxoplasma / Q000276:immunology; D014123:Toxoplasmosis / Q000175:diagnosis*
D000906:Antibodies
La méthode ELISA dans le diagnostic sérologique de la toxoplasmose humaine.