title: Demonstration of particulate accumulations in solid tissue.
abstract: We describe a histological clearing procedure involving partial tissue hydrolysis by pancreatin and demonstrate its application. Changes in distribution of 5.7-micrometers latex particles in mouse liver and heart muscle 6 months after i.v. latex injection were quantitated. The method is useful for studied of long-term redistribution of particles in vivo and for locating large, infrequent particles which are difficult to find by ordinary tissue-sectioning technique.
mesh_terms: D000818:Animals; D005260:Female; D006652:Histological Techniques; D007840:Latex / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008863:Microspheres; D009206:Myocardium / Q000378:metabolism*; D010194:Pancreatin
chemical_list: D007840:Latex; D010194:Pancreatin