Absence of ultraviolet-inducible DNA polymerase I-like activity in Escherichia coli strains harbouring R plasmids.
No DNA polymerase I-like activity was found associated with the ultraviolet (u.v.)-protecting plasmids R205, R46 or pKM101 in either uninduced or u.v.-induced wild-type or DNA polymerase I-deficient strains of Escherichia coli. Nor was any plasmid-associated polymerase activity detectable in similar systems containing u.v.-irradiated DNA as template. However, plasmids R205, R46 and pKM101 still increased survival and mutagenesis of the polymerase I-deficient E. coli strain after u.v. irradiation.
D004256:DNA Polymerase I / Q000096:biosynthesis / Q000378:metabolism*; D004269:DNA, Bacterial / Q000096:biosynthesis; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004790:Enzyme Induction / Q000528:radiation effects; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D009154:Mutation; D011815:R Factors; D013698:Templates, Genetic; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase