title: The myosin filament. VIII. Preservation of subfilament organization.
abstract: Different multistage fixation procedures patterned after the procedure described by Reedy (1976) have been evaluated with regard to preservation of subfilament structural organization along the length of the vertebrate skeletal myosin filament. The criteria used for evaluation of structural preservation include (a) the precision of subfilament organization as reflected by the quality of the optical diffraction patterns obtained from images of transverse sections of the myosin filament and (b) the clarity with which structural differences in serial transverse sections along the myosin filament could be defined.
mesh_terms: D000818:Animals; D003584:Cytological Techniques; D007695:Killifishes / Q000033:anatomy & histology; D008854:Microscopy, Electron; D009132:Muscles / Q000648:ultrastructure*; D009218:Myosins / Q000302:isolation & purification*; D055095:Optics and Photonics
chemical_list: D009218:Myosins