Application to mammalian tissues of the chemiluminescent method for detecting acetylcholine.
It is now possible to extend to mammalian tissues the chemiluminescent acetylcholine assay. Mammalian tissue extracts must be treated with oxidants (which is not necessary for electric organ extracts). The assay can then be performed as previously described (acetylcholinesterase hydrolyses acetylcholine; choline oxidase converts choline to betaine and H2O2, which gives off light in the presence of luminol and peroxidase). It is also shown that release experiments can be performed on mammalian tissue slices (mouse caudate nucleus) after the slice is washed in oxygenated saline solutions.
D000109:Acetylcholine / Q000032:analysis*; D000110:Acetylcholinesterase; D000429:Alcohol Oxidoreductases; D000818:Animals; D001923:Brain Chemistry; D002421:Caudate Nucleus / Q000032:analysis; D006735:Horseradish Peroxidase; D008163:Luminescent Measurements; D008165:Luminol; D008722:Methods; D051379:Mice; D009132:Muscles / Q000032:analysis*; D010084:Oxidation-Reduction
D008165:Luminol; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D006735:Horseradish Peroxidase; D000110:Acetylcholinesterase; D000109:Acetylcholine