title: Semisynthetic peptides and proteins.
abstract: Semisynthesis provides a flexible approach for using chemical synthesis to produce large, biologically active polypeptides and analogues. Currently developing improvements in the basic methods used, including polypeptide fragmentation, peptide synthesis, and reconstitution of synthetic and native components, make this overall approach applicable to a variety of species. Sequence modification through semisynthesis thus provides a flexible route to explore the code of rules whereby primary structure directs higher order properties of folded conformation and biological function of large peptides and proteins. The fruits of this endeavor, an understanding of how these macromolecules work, and therein, a basis for design of new structures that ultimately may be produced directly or by recombinant DNA methods, have begun to emerge.
mesh_terms: D000595:Amino Acid Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D004798:Enzymes / Q000138:chemical synthesis; D005288:Ferredoxins / Q000138:chemical synthesis; D005818:Genetic Engineering; D006420:Hemeproteins / Q000138:chemical synthesis; D006728:Hormones / Q000138:chemical synthesis; D007136:Immunoglobulins / Q000138:chemical synthesis; D010446:Peptide Fragments; D010447:Peptide Hydrolases; D010455:Peptides / Q000138:chemical synthesis; D011480:Protease Inhibitors / Q000138:chemical synthesis; D011487:Protein Conformation; D011506:Proteins / Q000138:chemical synthesis
chemical_list: D004798:Enzymes; D005288:Ferredoxins; D006420:Hemeproteins; D006728:Hormones; D007136:Immunoglobulins; D010446:Peptide Fragments; D010455:Peptides; D011480:Protease Inhibitors; D011506:Proteins; D010447:Peptide Hydrolases