title: Interactions among enzyme, antibody, drug, and time in enzyme immunoassays for drugs of abuse.
abstract: Published information on the major interactive variables of enzymic immunoassays for drugs, whether in urine or serum, is scanty. Using the Syva EMIT-dau Barbiturates assay as an example, we examined the following interactive variables with respect to test response: concentrations of enzyme, antibody, and drug; urine matrix; and time. The results suggest a process for optimizing this type of enzyme immunoassay. Transformation of the data gives indirect evidence of heterogeneous antibody reactions that are drug-concentration dependent.
mesh_terms: D000906:Antibodies; D001463:Barbiturates / Q000652:urine; D006801:Humans; D013287:Illicit Drugs / Q000032:analysis* / Q000652:urine; D007124:Immunoenzyme Techniques; D008433:Mathematics; D009113:Muramidase; D004364:Pharmaceutical Preparations / Q000032:analysis*; D013997:Time Factors
chemical_list: D000906:Antibodies; D001463:Barbiturates; D013287:Illicit Drugs; D004364:Pharmaceutical Preparations; D009113:Muramidase