title: Evaluation of the AutoMicrobic system for identification of glucose-nonfermenting gram-negative rods.
abstract: With the Enterobacteriaceae Plus Biochemical Card, the AutoMicrobic system (Vitek Systems, Inc., Hazelwood, Mo.) is purported to identify members of the family Enterobacteriaceae and seven species of glucose-nonfermenting gram-negative rods: Pseudomonas aeruginosa, P. fluorescens, P. putida, P. maltophilia, P. cepacia, and Acinetobacter calcoaceticus (saccharolytic and non-saccharolytic). The latter capability was examined in this investigation. Of 410 glucose-nonfermenting rods included in the identification profile, the AutoMicrobic system correctly identified 366 (89.3%). Of 62 glucose-nonfermenting organisms not included in the identification profile, 41 (66%) were correctly reported as "unidentified organism." The usefulness of the AutoMicrobic system-generated identification probability in establishing criteria for acceptance of identification is discussed.
mesh_terms: D000150:Acinetobacter / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D011336:Probability; D011549:Pseudomonas / Q000302:isolation & purification*
chemical_list: D005947:Glucose