Effect of antimicrobial agents on human polymorphonuclear leukocyte microbicidal function.
The effect of 19 antimicrobial agents on human polymorphonuclear leukocyte function was evaluated by chemiluminescence assays, yeast phagocytosis and killing, and lactate dehydrogenase release. Tetracycline and trimethoprim inhibited chemiluminescence and reduced killing at therapeutic concentrations of 2 microgram/ml. Cephalothin inhibited yeast killing at a concentration of 20 microgram/ml, but a significant depression of polymorphonuclear leukocyte chemiluminescence was encountered only at higher levels of 200 microgram/ml. The inhibition shown by these drugs was reversible. None of the other antimicrobial agents tested demonstrated inhibition of chemiluminescence, phagocytosis, or killing at usual clinical serum levels. No antimicrobial agent tested caused release of lactate dehydrogenase from polymorphonuclear leukocytes. The results suggest that therapeutic concentrations of tetracycline, trimethoprim, and cephalothin may inhibit optimal polymorphonuclear leukocyte microbicidal function.
D000890:Anti-Infective Agents / Q000494:pharmacology*; D001770:Blood Bactericidal Activity / Q000187:drug effects*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008163:Luminescent Measurements; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D010587:Phagocytosis; D012441:Saccharomyces cerevisiae / Q000276:immunology
D000890:Anti-Infective Agents; D007770:L-Lactate Dehydrogenase