[Protein yield in the rehydration of dehydrated Saccharomyces cerevisiae yeast cells].
The release of proteins was studied during rehydration of Saccharomyces cerevisiae cells dehydrated using two techniques which were characterized by different survival rate. A correlation was established between the loss of proteins and the survival rate of cells after dehydration and rehydration. Proteins with a molecular mass from 6000 to 70,000 daltons (low molecular weight proteins predominating) were found in the rehydration medium by the method of disc-electrophoresis in polyacrylamide gel in the presence of sodium dodecylsulfate. Moreover, the rehydration medium exhibited the activity of certain phosphohydrolases and dehydrogenases; this finding suggests that proteins with a higher molecular weight are present in the medium.
D003890:Desiccation; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis / Q000378:metabolism*; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000502:physiology*
D005656:Fungal Proteins
O vykhode belkov pri regidratatsii obezvozhennykh kletok drozhzheĭ Saccharomyces cerevisiae.