In vivo DNP tolerance induction by DNP-ficoll.
The kinetics of tolerance induction by administration of two different doses of DNP78-Ficoll on the anti-DNP IgM immune response in AJ mice was studied. The tolerogenic effects of 100 microgram of DNP78-Ficoll begin earlier and last longer than those of 10 microgram of DNP78-Ficoll as determined by the development of direct anti-DNP plaque-forming cells in the spleen and anti-DNP antibody titers in serum. In cell-transfer experiment, DNP tolerance was unstable. Transfer experiments did not produce detectable tolerogenic factors in serum. Antigen-antibody complexes, that tend to produce local suppressor effects, are discussed as mediators of tolerance.
D000818:Animals; D000917:Antibody Formation; D000936:Antigen-Antibody Complex / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D005260:Female; D005362:Ficoll / Q000031:analogs & derivatives / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D007108:Immune Tolerance; D007165:Immunosuppression Therapy; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D011134:Polysaccharides / Q000276:immunology*; D013154:Spleen / Q000166:cytology
D000936:Antigen-Antibody Complex; C031007:DNP-Ficoll; D011134:Polysaccharides; D005362:Ficoll