[Immunohistochemical localization of cytochrome P-450 in rat liver during phenobarbital induction].
Rabbit antisera against cytochrome P-450 were obtained. Cytochrome P-450 was isolated from the liver of rats preliminarily treated with phenobarbital (PB). Antiserum depleted by microsomal fraction of the normal liver was made use of to obtain antibodies for localization of P-450PB in liver tissue using the unlabeled antibody immunoperoxidase technique. Subject to study were paraffin liver slices from rats sacrificed 2-48 hours after intraperitoneal administration of PB. In the majority of intact liver cells, the staining was not seen, slightly exceeding the control level only in the narrow zone adjacent to the central veins. The intensity of staining increased and the zone of stained cells got larger during PB induction. This process reached the maximum after 24 hours. By that time the staining around the central veins was fairly intensive and progressively diminished towards lobule periphery, while only 1-2 cell layers around the peripheral vessels were slightly stained.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D008099:Liver / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats
D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital
Immunogistokhimicheskaia lokalizatsiia tsitokhroma P-450 v pecheni krysy v protsesse induktsii fenobarbitalom.