Ultrasonic decalcification of bone.
Decalcification of bone specimens of 2-5 mm thickness can be achieved in 5 hours or less when the decalcifying fluids are agitated by ultrasonic energization. The morphology of hard and soft tissue is extremely well preserved by this procedure. Acid or chelating decalcifiers may be used and the application of combined fixation-chelation permits routinely many histochemical procedures previously requiring special handling.
D001842:Bone and Bones / Q000473:pathology*; D003648:Decalcification Technique / Q000295:instrumentation*; D006801:Humans; D008722:Methods; D014465:Ultrasonics