Isopentenylation of both cytoplasmic and mitochondrial tRNA is affected by a single nuclear mutation.
Cytoplasmic rRNA from the yeast mutant, mod5-1 is deficient in the modified base isopentenyladenosine and consequently migrates differently from isopentenylated wild type tRNAs on certain chromatographic systems. To determine if the mod5-1 mutation affects mitochondrial tRNA structure, the migration of mitochondrial tRNA from mod5-1 and wild type cells has been compared by reverse-phase chromatography. We conclude from this analysis that the single nuclear mutation that affects the isopentenylation of cytoplasmic tRNA also affects the isopentenylation of mitochondrial tRNA.
D000475:Alkenes / Q000378:metabolism*; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D002467:Cell Nucleus / Q000032:analysis*; D004926:Escherichia coli / Q000201:enzymology; D008928:Mitochondria / Q000032:analysis*; D009154:Mutation; D012343:RNA, Transfer / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
C025669:2-isopentenyl tRNA; D000475:Alkenes; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases