Identification of beta-cell-trophin, a peptide of the pituitary pars intermedia which stimulates insulin secretion in plasma from genetically obese (ob/ob) mice.
The influence of plasma from genetically obese (ob/ob) and lean (+/+) mice on insulin secretion has been studied by perifusion of collagenase-prepared pancreatic islets maintained for 48 h in culture. Insulin secretion was measured at 2-min intervals and plasma from the ob/ob mice not from the +/+ mice rapidly stimulated insulin release, reaching a maximum in 2-4 min and falling to basal levels in about 10 min. Experimental evidence is given indicating that the plasma insulin secretagogue is identical to beta-cell-trophin, a peptide of the pituitary pars intermedia which stimulates insulin secretion. The evidence is based on (1) the antigenic properties of the peptides (both cross-react with a -COOH terminal ACTH antiserum raised to the 17-39 moiety of ACTH), (2) identical chromatographic separation on Biogel columns and on reverse-phase high pressure liquid chromatography and (3) the similarity of their insulin releasing action from perifused islets.
D000324:Adrenocorticotropic Hormone; D000818:Animals; D007328:Insulin / Q000378:metabolism*; D007515:Islets of Langerhans / Q000378:metabolism*; D051379:Mice; D008820:Mice, Obese; D009924:Organ Culture Techniques; D010446:Peptide Fragments; D010477:Perfusion; D010903:Pituitary Gland, Anterior / Q000378:metabolism; D010907:Pituitary Hormones / Q000378:metabolism*
D007328:Insulin; D010446:Peptide Fragments; D010907:Pituitary Hormones; C026834:beta-cell tropin; D000324:Adrenocorticotropic Hormone