Modified fluctuation test for the direct detection of mutagens in foods with Salmonella typhimurium TA98.
A modified fluctuation test (Green) with the Ames' tester strain Salmonella typhimurium TA98 has been examined for sensitivity to histidine feeding and for detection of minimal concentrations of daunomycin and S9 activated benzo[a]pyrene. The fluctuation test was found operable over a range of histidine concentrations between 0.25 and 1.25 microgram/ml using 48 tube assays and microtitre plates with 120 wells. The agar plate method yielded a comparable operational range for histidine concentration. With daunomycin, the microtiter fluctuation test was 48-fold greater in sensitivity than the macroscale fluctuation test. With benzo [a] pyrene, the microtiter fluctuation test was 4.8-fold greater in sensitivity than the macroscale test. The microtiter assay was 2.4 and 2.5-fold more sensitive than the plate and treat method with daunomycin and benzo [a] pyrene respectively.
D001431:Bacteriological Techniques; D001580:Benzopyrenes / Q000494:pharmacology; D003630:Daunorubicin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005502:Food; D006639:Histidine / Q000494:pharmacology; D009152:Mutagenicity Tests / Q000379:methods*; D009154:Mutation; D012486:Salmonella typhimurium / Q000235:genetics
D001580:Benzopyrenes; D006639:Histidine; D003630:Daunorubicin