[Use of the ELISA (enzyme-linked immunosorbent assay) method in detection of anti-toxoplasma IgG and IgM].
The enzyme-linked immunosorbent assay (ELISA) has been applied for detecting anti-toxoplasma serum IgG and IgM. ELISA was carried out with alkaline phosphatase-labeled anti-IgG and anti-IgM antibodies. Both conjugates specificity was investigated by sucrose density gradient fractionating of an immunofluorescence positive serum and by ELISA testing of all the fractions for IgG and IgM. A good agreement of ELISA results from 400 sera was found when they were compared with others obtained by several serological methods: direct agglutination, complement fixation, IgG/IgM-IIF. Occasionally false-positive IgM-ELISA and IgM-IIF results were observed, probably due to non specific IgM antibodies. Absorption of sera with whole parasites resulted in a removal of specific IgM, whilst false-positive IgM-ELISA reactions were unaffected. ELISA procedure is simple and rapid to carry out in a large scale and it seems to be for routine purposes an adequate substitute for toxoplasmosis IIF test. The possibility to quantitate ELISA results would give the opportunity to standardize the test not only in one, but even among several laboratories.
D000372:Agglutination Tests; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000276:immunology
D007075:Immunoglobulin M; D007073:Immunoglobulin E
Applicazione del metodo ELISA (enzyme-linked immunosorbent assay) alla ricerca di IgG e IgM anti-toxoplasma.