title: Glycoprotein synthesis and migration in beta cells of the islets of Langerhans as shown by quantitative light- and electron-microscopic radioautography.
abstract: L-3H-fucose was injected intravenously into rats that were killed from 10 min to 7 days after isotope administration. Semi-thin and thin sections of the islets of Langerhans were processed for light- and electron-microscopic radioautography, respectively, and analyzed quantitatively. L-3H-fucose was incorporated into newly synthesized glycoproteins in the Golgi apparatus of the beta cells and subsequently labeled glycoproteins migrated to secretory granules and plasma membrane. Therefore, some of the glycoproteins synthesized by the beta cells of the islets of Langerhans are destined for the renewal of plasma membrane. Although the labeling of the secretory granules was clearly demonstrated, it was not possible to decide if the newly formed glycoproteins are incorporated into the content or into the membrane of the granule. Thus, the fate as well as the function of secretory-granule glycoproteins could not be determined precisely. Several hypothesis concerning the presence of glycoproteins in the secretory granules in relation with insulin metabolism are considered.
mesh_terms: D000818:Animals; D001345:Autoradiography; D003594:Cytoplasmic Granules / Q000378:metabolism; D005643:Fucose / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron; D051381:Rats
chemical_list: D006023:Glycoproteins; D007328:Insulin; D005643:Fucose