Phenformin stimulation of insulin binding to human cultured lymphocytes.
The effects of the oral hypoglycemic agent, phenformin, were studied on the binding of 125I-insulin to its receptors in IM-9 human cultured lymphocytes. Three h after the addition of 5 microgram/ml of phenformin to these cells there was detectable stimulation of 125I-insulin binding; maximal effects were seen after 18 h. A detectable effect of phenformin was seen at 1 microgram/ml and maximal effects were seen at 5 microgram/ml. These studies demonstrate therefore than phenformin increases the binding of 125I-insulin to human cultured lymphocytes, and raise the possibility that phenformin could act in vivo to regulate insulin receptors.
D002478:Cells, Cultured; D006801:Humans; D007328:Insulin / Q000097:blood*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D010629:Phenformin / Q000494:pharmacology*; D011972:Receptor, Insulin / Q000378:metabolism*
D007328:Insulin; D010629:Phenformin; D011972:Receptor, Insulin