title: Sequences at the 3' ends of yeast viral dsRNAs: proposed transcriptase and replicase initiation sites.
abstract: ScV is a double-stranded RNA virus of yeast consisting of two separately encapsidated dsRNAs (L and M). ScV-1 and ScV-2 are two dsRNA viruses present in two different yeast killer strains, K1 and K2. Our 3' end sequence analysis shows that the two sets of viral dsRNAs from ScV-1 and ScV-2 are very similar. Consensus sequences for transcriptase and replicase initiation are proposed. A stem and loop structure with a 3' terminal AUGC sequence, like that of several plant virus plus strand RNAs, is present at the putative replicase initiation site of  one of the yeast viral RNA plus strands.
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*; D012328:RNA Viruses / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism*; D012441:Saccharomyces cerevisiae
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase; D012321:DNA-Directed RNA Polymerases