A rapid antibody assay system for screening hybridoma cultures.
A procedure for the rapid qualitative assay of large numbers of antibody samples is described which has been extensively used for primary screening of mouse spleen cell-myeloma hybrids making monoclonal antibodies. It is a handling modification of standard indirect immunofluorescence of immunoperoxidase techniques which involves in situ localisation of antigens in a fixed and dried monolayer of cells on plastic culture dishes. The method is quick and economical and does not depend on hazardous reagents or specialised detection equipment.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology*; D007124:Immunoenzyme Techniques; D051379:Mice; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000911:Antibodies, Monoclonal