[Proliferation kinetics of PHA-stimulated lymphocytes after whole-body irradiation of patients with non-Hodgkin lymphomas (author's transl)].
A number of patients suffering from non-Hodgkin lymphomas received a whole-body irradiation with 15 rad per fraction (10 fractions). Before and after every second fraction, the proliferation kinetics of the peripheral lymphocytes was examined after PHA stimulation (DNA content per culture as cell number equivalent, incorporation of thymidine into DNA after isolation of DNA, distribution of cells within the cell cycle). After a total dose of 60 rad, the lymphocytes of nine among twenty patients showed a decrease of all proliferation-kinetic parameters. This decrease was followed by an increase up to values lying partly above the initial values. A second group did not show this increase. No correlations could be found between these changes and the clinical course or the symptomatology. The formation of micronuclei in lymphocytes of six patients could be examined before the whole-body irradiation and after an in-vitro irradiation. The results suggest the possibility of an individual determination of the radiosensibility of non-Hodgkin lymphomas.
D000328:Adult; D000368:Aged; D002455:Cell Division / Q000528:radiation effects*; D006801:Humans; D008214:Lymphocytes / Q000473:pathology*; D008223:Lymphoma / Q000473:pathology*; D008875:Middle Aged; D010835:Phytohemagglutinins; D011829:Radiation Dosage; D014916:Whole-Body Irradiation
D010835:Phytohemagglutinins
Proliferationskinetik PHA-stimulierter Lymphozyten von Non-Hodgkin-Lymphom-Patienten nach Ganzkörperbestrahlung.