Electrochemical measurements of cell populations.
Determination of cell growth was carried out by a polarographic system. The system was constructed of two platinum electrodes, a saturated calomel electrode, and a thermistor electrode. Responses of the system to the dissolved oxygen, pH, and temperature were examined. Cell growth of S. cerevisiae and M. olivoasterospora was monitored continuously by this system. In addition, this polarographic system could be applied to the measurements of cell populations of the human cancer cell L-1210 and mouse leukocytes. The measureable range for these animal cells was approximately 10(3)--10(5) cells/ml.
D000818:Animals; D002277:Carcinoma; D002460:Cell Line; D002468:Cell Physiological Phenomena; D004566:Electrodes; D005285:Fermentation; D006801:Humans; D007939:Leukemia L1210 / Q000503:physiopathology; D008722:Methods; D051379:Mice; D008847:Micromonospora / Q000502:physiology; D009062:Mouth Neoplasms; D010944:Plants; D011199:Potentiometry; D012441:Saccharomyces cerevisiae / Q000502:physiology