title: Lipid metabolism of isolated mouse pancreatic islets maintained in culture at different glucose concentrations.
abstract: The lipid metabolism of isolated mouse pancreatic islets, which had been cultured for one week at 3.3 or 16.7 mM glucose, was examined in short-term experiments performed at the end of the culture period. It was found that: a) high-glucose cultured islets oxidized palmitate at a higher rate: and b) both groups of cultured islets increased the rate of incorporation of (U-14C)-glucose into triacylglycerols and phospholipids in response to an acute glucose challenge. This latter effect was, however, more pronounced after culture at a high glucose concentration. The present data suggest that long-term exposure to high concentrations of glucose leads to a general increase of islet oxidative metabolism. Moreover, high glucose culture seems to induce an increased conversion of carbohydrates into triacylglycerols and membrane phospholipids.
mesh_terms: D000818:Animals; D046508:Culture Techniques; D005947:Glucose / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000378:metabolism*; D050356:Lipid Metabolism; D008297:Male; D051379:Mice; D010084:Oxidation-Reduction; D010169:Palmitic Acids / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis; D014280:Triglycerides / Q000096:biosynthesis
chemical_list: D010169:Palmitic Acids; D010743:Phospholipids; D014280:Triglycerides; D005947:Glucose