Detection of antibody to carcinoembryonic antigen by the hemolytic plaque assay.
A modification of the standard hemolytic plaque-forming cell assay is described. The conjugation of a soluble antigen, carcinoembryonic antigen, to the membrane of the sheep red blood cell was accomplished and used as the indicator system to detect antibody-forming cells. The specificity of the assay was determined by cross-reacting studies and competitive inhibition by antigen blocking.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000276:immunology*; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D002460:Cell Line; D006224:Cricetinae; D006462:Hemolytic Plaque Technique; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012756:Sheep
D000906:Antibodies; D002272:Carcinoembryonic Antigen