Isolation of genome-enzyme complex from cytoplasmic polyhedrosis virus of silkworm Bombyx mori.
The genome-enzyme complex is isolated from the silkworm cytoplasmic polyhedrosis virus with DEAE-Sephadex column chromatography after ultraviolet irradiation. The genome-enzyme complex shows both RNA-polymerase and methyltransferase activities while using 3H-UTP and [3H-methyl]-S-adenosyle-L-methionine as substrates. Like the double-stranded RNA genome of CPV, the genome-enzyme complex could be separated into nine segments on polyacrylamide gel electrophoresis. It is demonstrated that the RNA polymerase and methyl-transferase are tightly bound to the double-stranded RNA genome and that the individual segments of the genome-enzyme complex all possess RNA-polymerase and methyltransferase activities. It appears that each segment of the double-stranded RNA genome is transcribed independently.
D012831:Bombyx; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005814:Genes, Viral; D007304:Insect Viruses / Q000235:genetics*; D008780:Methyltransferases / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D008780:Methyltransferases; D012321:DNA-Directed RNA Polymerases