Increase of low-Km NADPH-dependent aldehyde reductase activity in the brain by chronic treatment of rats with barbital.
Kinetics studies showed two apparent Km values for p-nitrobenzaldehyde. The high-Km aldehyde reductase (ALR) had a lower Ki value for barbital than the low-Km ALR. The value for maximal velocity (Vmax) for the high-Km ALR was not changed by chronic barbital treatment. However, the Vmax value for the low-Km ALR was significantly increased by chronic barbital treatment. These results suggest that during barbital treatment low-Km ALR which is less sensitive to barbital is induced in compensation for the decreased aldehyde metabolizing capacity of brain.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001462:Barbital / Q000494:pharmacology*; D001463:Barbiturates / Q000494:pharmacology*; D001921:Brain / Q000201:enzymology*; D003600:Cytosol / Q000378:metabolism; D007527:Isoenzymes; D007700:Kinetics; D008297:Male; D009249:NADP / Q000378:metabolism*; D051381:Rats
D001463:Barbiturates; D007527:Isoenzymes; D009249:NADP; D001462:Barbital; D000429:Alcohol Oxidoreductases; C019642:alcohol dehydrogenase (NADP+)