A nonspecific inhibitor of macrophage migration in cell-free ascites of rat Zajdela hepatoma. I. Biological studies on inhibitor formation.
Wistar rats were inoculated with Zajdela ascites hepatoma. A nonspecific inhibitor of macrophage migration was detected in the cell-free ascitic fluid during the early phase of tumor growth (at day 4-8). Dose-response curves of cell-free ascitic fluids revealed peak migration inhibitory activity at dilutions up to 1:640. The migration inhibitory activity presents a nondialyzable protein, unaffected by freezing, lyophilization, incubation at 60 degrees C for 30 min or pH 2 treatment. In all probability, the presumed factor is produced by the tumor cells as revealed by studies in vitro.
D000818:Animals; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D008114:Liver Neoplasms, Experimental / Q000503:physiopathology*; D008263:Macrophage Migration-Inhibitory Factors / Q000302:isolation & purification* / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D008263:Macrophage Migration-Inhibitory Factors