Effect of lysosomotropic amine, chloroquine, on the radioactive incorporation pattern of glycosaminoglycans in human skin fibroblasts.
Control and chloroquine treated fibroblast cultures were incubated with 14C-glucosamine. The cells were separated from the medium and the pericellular matrix. The S-carboxymethylproteins in the medium were hydrolysed with trypsin and pronase. The glycosaminoglycans were treated with hyaluronidase, chondroitin AB-lyase and chondroitin ABC-lyase. The macromolecular 14C label per cell in the medium was higher in the chloroquine treated cultures than in the controls. The incorporation of the 14C-hexosamines per cell into the pericellular and intracellular compartment was not modified. The increased 14C-label in the medium of fibroblast exposed to chloroquine accounted for the increased incorporation of the 14C precursor into chondroitin 4 and 6 sulfates and dermatan sulfate.
D002478:Cells, Cultured; D002738:Chloroquine / Q000494:pharmacology*; D002850:Chromatography, Gel / Q000379:methods; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism*; D006595:Hexosamines / Q000378:metabolism; D006801:Humans; D008247:Lysosomes / Q000187:drug effects*
D006025:Glycosaminoglycans; D006595:Hexosamines; D002738:Chloroquine; D005944:Glucosamine