[Effect of periodate oxidation on activity of acid alpha-glucosidase from human liver].
Treatment with sodium metaperiodate caused oxidation of carbohydrate residues in acid alpha-glucosidase (gamma-amylase). Efficiency of the oxidation was monitored by a decrease in specific binding of alpha-glucosidase with concanavaline A-Sepharose. The degree of oxidation reached 96% in a medium containing 0,011 M NaIO4, pH 5.5, within 120 min. The residual activity of oxidized alpha-glucosidase was about 77%. The rate of oxidation and residual enzymatic activity were shown to depend also on concentration of sodium metaperiodate and duration of the reaction.
D055598:Chemical Phenomena; D002621:Chemistry; D005959:Glucosidases / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007452:Iodates; D007455:Iodine; D008099:Liver / Q000201:enzymology*; D010084:Oxidation-Reduction; D000520:alpha-Glucosidases / Q000378:metabolism*
D007452:Iodates; D007455:Iodine; D005959:Glucosidases; D000520:alpha-Glucosidases
Sokhranenie fermentativnoĭ aktivnosti kisloĭ al'fa-gliukozidazy pecheni cheloveka, podvergnutoĭ perĭodatnomu okisleniiu.