Cell-mediated cytotoxicity toward canine kidney epithelial cells.
Cellular cytotoxicity toward kidney cell targets has been studied in a model using in vitro cultured canine kidney cells obtained after perfusion trypsinization of kidneys from one-haplotype-mismatched beagles. To study whether cytotoxic effector cells recognize identical antigens on kidney cells as on phytohemagglutinin (PHA)-stimulated lymphoblasts, adsorption studies with different monolayers have been performed. Both leukocyte and kidney cell monolayers reduced cytotoxicity against 51Cr-labeled PHA-stimulated lymphoblasts very effectively. The average reduction of cytotoxicity was 86% in six consecutive experiments after one adsorption on either one of these two types of target-specific monolayers. Nonspecific monolayers reduced cytotoxicity only for 13%. Specific kidney cell monolayers reduced cytotoxicity against kidney cells almost completely, however leukocyte monolayers reduced cytotoxicity toward kidney cells for only 40%. There results and cold target inhibition data strongly suggest that kidney cells present antigens to which a selective population of cytotoxic T lymphocytes (CTLs) is directed. These CTLs are not cytotoxic for PHA-stimulated lymphoblasts. It is discussed whether the relevant antigens on the kidney cells are organ-specific antigens comparable to the endothelial monocyte antigen system as described by Moreas and Stastny or that class II antigens are involved in cytotoxicity toward kidney cells.
D000818:Animals; D001667:Binding, Competitive; D003602:Cytotoxicity, Immunologic; D004285:Dogs; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D007111:Immunity, Cellular; D007163:Immunosorbent Techniques; D007668:Kidney / Q000166:cytology / Q000276:immunology*; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000494:pharmacology
D010835:Phytohemagglutinins