A one-step procedure for preparation of classical pathway (C1q) and alternative pathway (factor D) depleted human serum.
A simple method for preparation of a serum depleted in both C1q and Factor D is described. The hemolytic activities of both pathways are completely abolished and can be fully restored using the respective purified complement components. Furthermore, this serum is useful for studying cell systems since blocking either complement pathway does not require chelating agents.
D001667:Binding, Competitive / Q000187:drug effects; D002614:Chelating Agents / Q000494:pharmacology; D003166:Complement Activating Enzymes / Q000172:deficiency*; D003167:Complement Activation; D015922:Complement C1q; D003176:Complement C3 / Q000032:analysis; D011416:Complement Factor D / Q000172:deficiency*; D003170:Complement Pathway, Alternative; D003171:Complement Pathway, Classical; D006461:Hemolysis; D006801:Humans; D007158:Immunologic Techniques
D002614:Chelating Agents; D003176:Complement C3; D015922:Complement C1q; D003166:Complement Activating Enzymes; C493488:CFD protein, human; D011416:Complement Factor D