The molecular composition of the volutin granule of yeast.
The volutin granule was isolated from yeast by disruption of freeze-dried cells in an organic solvent and density-gradient-gradient centrifugation. The granule is composed of two types of macromolecule, a linear-chain polyphosphate and four basic proteins, of molecular weights ranging from 10 000 to 20 000. In the dissolved granule these macromolecules are in a complex that is uniform by hydrodynamic criteria (s20,w = 22.3 S). The polyphosphate separated from this complex gives a single 31P n.m.r. resonance and in the analytical ultracentrifuge behaves as a monodisperse solute of molecular weight 245 000 +/- 1000. In the 31P n.m.r. spectrum of yeast used for its isolation, this polyphosphate accounts for 14% of total cell polyphosphate.
D000596:Amino Acids / Q000032:analysis; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002621:Chemistry; D004396:Coloring Agents / Q000302:isolation & purification; D003594:Cytoplasmic Granules / Q000032:analysis*; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D008670:Metals / Q000032:analysis; D011122:Polyphosphates / Q000032:analysis; D011506:Proteins; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D000596:Amino Acids; D004396:Coloring Agents; D046911:Macromolecular Substances; D008670:Metals; D011122:Polyphosphates; D011506:Proteins