Immunocytochemical identification of amyloid in formalin-fixed paraffin sections.
Formalin-fixed paraffin sections of livers, spleens and kidneys from patients with primary, secondary and familial amyloidosis as well as from a casein-induced murine amyloid model were analysed by an immunocytochemical (unlabeled antibody enzyme) method utilizing antisera to amyloid-related proteins. All amyloid deposits of all amyloid types showed positive reactions with anti-AP of the respective species. Positive reaction of anti-human AA to human secondary amyloid deposits and of anti-mouse AA to the deposits of casein-induced murine amyloid was also observed, but there was no species cross reactivity. No significant deposition of the reaction products was produced by anti-immunoglobulin light chains on deposits of any amyloid type, or by anti-AA in the tissues from primary or familial amyloidosis. The results indicate that amyloid proteins AA and AP can survive as antigens through routine histologic preparation, that anti-AP can be a universal marker for deposits of any amyloid type within the same species, and that AA-type amyloid can be identified by this method while there may as yet be no feasible universal marker for the AL-type at present.
D000682:Amyloid / Q000032:analysis*; D000686:Amyloidosis / Q000139:chemically induced / Q000473:pathology*; D000818:Animals; D002364:Caseins; D004195:Disease Models, Animal; D006652:Histological Techniques; D006801:Humans; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000473:pathology*; D008099:Liver / Q000473:pathology*; D051379:Mice; D008808:Mice, Inbred CBA; D000685:Serum Amyloid A Protein / Q000032:analysis; D013154:Spleen / Q000473:pathology*
D000682:Amyloid; D002364:Caseins; D000685:Serum Amyloid A Protein