Biosynthesis of crystallin by rabbit lens fiber cells. II. Effect of culture medium.
Freshly isolated lenses incubated in either M-199 alone or supplemented with serum or insulin were measured for alpha- and gamma-crystallin synthesis. It was found that synthesis of these crystallins were not altered by addition of either serum of insulin in the culture medium. The alpha-crystallin synthesis showed decreased synthetic activity after 1 day of culture, whereas gamma-crystallin synthesis maintained a comparatively similar rate of synthesis for 2 days.
D000818:Animals; D003459:Crystallins / Q000096:biosynthesis*; D003470:Culture Media; D066298:In Vitro Techniques; D007328:Insulin; D007908:Lens, Crystalline / Q000378:metabolism*; D010949:Plasma; D011817:Rabbits
D003459:Crystallins; D003470:Culture Media; D007328:Insulin