title: Requirement of Fnr and NarL functions for nitrate reductase expression in Escherichia coli K-12.
abstract: I used a chlC-lac operon fusion to study regulatory mutations which affect nitrate reductase expression in Escherichia coli. A NarL- mutant apparently lacks a nitrate-specific positive regulatory component. Furthermore, an fnr (nirR) mutation prevented enzyme induction under any conditions. These data are consistent with a two-step, positive control model for nitrate reductase regulation.
mesh_terms: D004274:DNA, Recombinant; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005809:Genes, Regulator; D009154:Mutation; D009565:Nitrate Reductases / Q000096:biosynthesis / Q000235:genetics*; D009876:Operon; D010100:Oxygen / Q000494:pharmacology; D014161:Transduction, Genetic
chemical_list: D004274:DNA, Recombinant; D009565:Nitrate Reductases; D010100:Oxygen