Effect of carbon source on growth rate and phospholipid composition of Escherichia coli 15T- and an unsaturated fatty acid auxotroph.
Escherichia coli 15T- was grown with glucose, succinic acid, aspartic acid, oleic acid, and oleic plus aspartic acids as carbon sources, and a fatty acid auxotroph derived from 15T- was grown on oleic acid and oleic plus aspartic acids. The doubling time, phospholipid composition, phosphorus content, and the fatty acid composition of the phospholipids of cells in each of the media were determined. In all cases, phosphatidylethanolamine was the major phospholipid present; but with 15T- its concentration was inversely proportional to the doubling time in unsupplemented media. With the auxotroph the phosphatidylethanolamine concentration was essentially unchanged with growth. Total lipid phosphorus was inversely proportional to doubling time, an effect particularly evident with the auxotroph. Without oleic acid supplementation, the major effects of carbon source on fatty acid composition are decreases in the content of palmitoleic acid and increases in the content of cis-9, 10-methylene hexadecanoic acid as growth rate decreases. Oleic acid supplementation elevated 18:1 fatty acid content in both 15T- and the auxotroph.
D001224:Aspartic Acid / Q000494:pharmacology; D003470:Culture Media; D004926:Escherichia coli / Q000032:analysis / Q000254:growth & development* / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D005947:Glucose / Q000494:pharmacology; D007700:Kinetics; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology*; D010714:Phosphatidylethanolamines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D013386:Succinates / Q000494:pharmacology; D019802:Succinic Acid
D003470:Culture Media; D005227:Fatty Acids; D009829:Oleic Acids; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D013386:Succinates; D019301:Oleic Acid; D001224:Aspartic Acid; D019802:Succinic Acid; D005947:Glucose