title: Iodinated [A1-N gamma-(4-azidophenylacetyl)-D-alpha, gamma-diaminobutyric acid]insulin: its preparation and properties.
abstract: The synthesis of a photo-sensitive analogue of pork insulin (A1-N gamma-(4-azidophenylacetyl)-D-alpha, gamma-diaminobutyric acid]insulin is reported. It could be shown that this analogue forms specific cross-links to binding proteins on irradiation with UV light. Furthermore, it was shown that the photo-label itself was not iodinated, nor did it influence the normal distribution of iodination. It can be concluded that this derivative bears all of the characteristics necessary for specifically labeling membrane-bound receptors.
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001386:Azides / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000494:pharmacology; D001681:Biological Assay; D005947:Glucose / Q000378:metabolism; D007202:Indicators and Reagents; D007328:Insulin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis; D011487:Protein Conformation; D051381:Rats; D013055:Spectrophotometry, Infrared; D013552:Swine
chemical_list: D001386:Azides; D007202:Indicators and Reagents; D007328:Insulin; D008055:Lipids; C035506:insulin, 4-(azidophenylacetyl)-2,4-diaminobutyric acid(A1)-; D005947:Glucose