title: Membrane potential stimulated binding of the maltose-binding protein to membrane vesicles of Escherichia coli.
abstract: We show in this study that radioactively labelled purified maltose-binding protein of Escherichia coli binds specifically to membrane vesicles, in the presence of maltose. When a potential is imposed across the membrane, the specific binding is increased, dependent on maltose, abolished in a mutant defective in the tar-gene product, one of the methyl-accepting chemotaxis proteins.
mesh_terms: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029968:Escherichia coli Proteins; D058619:Maltose-Binding Proteins; D008564:Membrane Potentials; D009004:Monosaccharide Transport Proteins; D009154:Mutation; D011485:Protein Binding; D013997:Time Factors
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; C461599:maltose transport system, E coli