A rosette assay for identification of Ia-like alloantigens on chicken lymphoid cells.
An Ia-rosette assay for the detection of Ia-like alloantigens on the chicken lymphoid cell surface is described. The method is based on the ability of cells treated with alloantiserum to Ia and then with rabbit antiserum against the Fc portion of chicken IgG, to form rosettes with sheep erythrocytes (SRBC) coated with chicken anti-SRBC IgG antibody. Interference with Ia-rosette formation by Fc IgG receptors was eliminated by pronase treatment which removes Fc receptor activity without affecting Ia antigens. The Ia-rosette assay is at least as sensitive as the triple-layer immunofluorescence test for identification of Ia antigens. In addition, the assay allows morphological study and separation of the rosetting cells.
D000818:Animals; D002060:Bursa of Fabricius / Q000276:immunology; D002645:Chickens; D005260:Female; D005455:Fluorescent Antibody Technique; D000949:Histocompatibility Antigens Class II; D007519:Isoantigens; D008214:Lymphocytes / Q000276:immunology*; D011402:Pronase / Q000494:pharmacology; D011817:Rabbits; D012397:Rosette Formation; D012756:Sheep
D000949:Histocompatibility Antigens Class II; D007519:Isoantigens; D011402:Pronase