[Transfection of bacterial cells by DNA of phage M13 entrapped in phospholipid vesicles].
The possibility of transfection of bacterial cells by phage M13 DNA entrapped into phospholipid vesicles (liposomes) has been studied. Two types of liposomes differing in size were used. Entrapped DNA was transferred by liposomes into Ca2+-treated E. coli cells. Efficiency of the transfection in the case of small (ca. 400 A) liposomes was 2--3 orders of magnitude higher than that of free DNA extracted from such liposomes.
D002118:Calcium / Q000494:pharmacology; D003090:Coliphages / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D008081:Liposomes; D008968:Molecular Conformation; D014162:Transfection
D004279:DNA, Viral; D008081:Liposomes; D002118:Calcium
Transfektsiia bakterial'nykh kletok zakliuchennoĭ v fosfolipidnye vezikuly DNK faga M13.