title: Screening for deficiencies in the classical and alternative pathways of complement by hemolysis in gel.
abstract: Two assays based on hemolysis in gel were assessed for screening complement (C) component deficiencies. In one assay sensitized sheep erythrocyte (EA) were incorporated in agarose gel containing Ca2+ and Mg2+, in the other guinea pig erythrocytes (GpE) were used in the presence of Mg2+and EGTA. With few exceptions, fresh samples from healthy individuals produced homogeneous areas of complete hemolysis in both assays. Clearly aberrant patterns were observed in approximately 4% of healthy blood donors. Sera from patients having complete deficiencies of Clq, C2 or C4 produced clear lysis of GpE only, whereas in sera lacking C3 or C8 lysis was grossly impaired in both assays. Properdin deficient serum produced very slight lysis of GpE but normal lysis of EA. Reconstitution of these C-deficient sera gave normal lysis. Together, the two assays supplement immunochemical C3 and C4 determinations for screening out C disorders.
mesh_terms: D000818:Animals; D003166:Complement Activating Enzymes / Q000172:deficiency; D003167:Complement Activation; D015922:Complement C1q; D003175:Complement C2 / Q000172:deficiency; D003176:Complement C3 / Q000172:deficiency; D003181:Complement C4 / Q000172:deficiency; D003185:Complement C8 / Q000172:deficiency; D003170:Complement Pathway, Alternative; D003171:Complement Pathway, Classical; D006168:Guinea Pigs / Q000097:blood; D006462:Hemolytic Plaque Technique; D006801:Humans; D012756:Sheep / Q000097:blood
chemical_list: D003175:Complement C2; D003176:Complement C3; D003181:Complement C4; D003185:Complement C8; D015922:Complement C1q; D003166:Complement Activating Enzymes