Ca2+-uptake properties of two populations of mitochondria from normal and denervated rat soleus muscle.
Ultraturrax- and Nagarse-released populations of mitochondria were characterized with respect to their Ca2+-uptake activities (i) by means of the indirect polarographic technique and (ii) directly by the 45Ca Ruthenium Red-quench method of Reed & Bygrave [(1974) Biochem. J. 140, 143-155]. The denervated-muscle subsarcolemmal and intermyofibrillar mitochondrial fractions displayed markedly decreased rates and capacities for Ca2+ uptake compared with their respective controls. The implications of these findings with respect to the process of cell necrosis are discussed.
D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000378:metabolism*; D009121:Muscle Denervation; D011048:Polarography; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical; D013381:Subtilisins / Q000494:pharmacology
D013381:Subtilisins; D002118:Calcium