title: Ether inhibition of ethanol metabolism in isolated rat liver parenchymal cells.
abstract: The effect of diethyl ether on ethanol metabolism was studied in isolated rat hepatocytes and ether was found to inhibit ethanol oxidation in a dose-dependent manner. At ethanol concentrations of approximately 30 mM, diethyl ether inhibited ethanol oxidation by approximately 58%, 40%, and 20% at ether concentrations of 30, 20, and 10 mM, respectively. This inhibition was also seen at a low ethanol concentration (5.4 mM) and in pyruvate (5 mM)-stimulated hepatocytes which exhibited increased rates of ethanol metabolism closer to in vivo rates. Accumulation of acetaldehyde from ethanol in cyanamide (400 micron M)-treated hepatocyte suspensions was also reduced by approximately 16% by 30 mM ether. It was concluded that inhibition of ethanol metabolism by diethyl ether might be of practical importance in studies of ethanol metabolism in ether anesthetized animals.
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism; D000818:Animals; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000378:metabolism*; D004986:Ether / Q000494:pharmacology*; D005019:Ethyl Ethers / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005019:Ethyl Ethers; D004986:Ether; D000431:Ethanol; D000079:Acetaldehyde