Induction of immunologic memory by a lipopolysaccharide-protein complex isolated from Fusobacterium necrophorum: cellular response.
The ability of a lipopolysaccharide protein (LPS-P) complex extracted from Fusobacterium necrophorum to establish immunologic memory in BALB/c mice splenocytes was demonstrated. The LPS-P molecule differed from the phenol water-extracted LPS because it contained approximately 12% protein. Initial experiments showed that primary and secondary spleen plaque-forming cell (PFC) responses to IV or IM injections of LPS-P were highly dose-dependent. Suitable primary doses stimulated significant (P less than 0.05) amounts of direct and direct + indirect PFC by postinoculation day (PID) 14 and primed the mice for an enhanced secondary response to small booster injections. When mice were inoculated with a suitable primary IM dose of aluminum hydroxide-precipitated LPS-P, significant amounts of direct and direct + indirect PFC were detectable through PID 120. Moreover, significant enhancement of these values was attained with an IV booster injection at PID 105. Primary IV inoculation with LPS-P produced similar results, although the primary response was not as persistent.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D005673:Fusobacterium / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D007117:Immunization, Secondary; D007156:Immunologic Memory; D007273:Injections, Intramuscular; D007275:Injections, Intravenous; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000276:immunology
D001426:Bacterial Proteins; D008070:Lipopolysaccharides