kappa/lambda Shifts do not occur during maturation of murine B cells.
We have used highly specific affinity-purified antibodies against mouse kappa- and lambda chains in conjunction with both immunofluorescence analysis on the FACS and enzymatic radioiodination of murine splenocytes and bone marrow cells. Our results indicate that the kappa/lambda ratio of cell surface immunoglobulin is constant during the ontogenetic development of B cells, i.e., a shift in the kappa/lambda ratio does not occur during B cell maturation. Our results suggest that the events controlling kappa and lambda expression on murine B cells are decided very early in ontogeny, before significant antigenic selection.
D000818:Animals; D000906:Antibodies; D001402:B-Lymphocytes / Q000166:cytology*; D001853:Bone Marrow / Q000276:immunology; D002454:Cell Differentiation; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D005260:Female; D005455:Fluorescent Antibody Technique; D006041:Goats; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D011817:Rabbits; D013154:Spleen / Q000276:immunology
D000906:Antibodies