Subcellular distribution of prolyl endopeptidase and cation-sensitive neutral endopeptidase in rabbit brain.
The subcellular distribution of prolyl endopeptidase, and of cation-sensitive neutral endopeptidase, two enzymes actively metabolizing many neuropeptides, was determined in homogenates of rabbit brain. The subcellular distribution of both enzymes was more similar to lactate dehydrogenase, a cytoplasmic enzyme marker, than to choline acetyltransferase, a synaptosomal marker. Only 35% of the activity of these two neutral endopeptidases was found in the crude mitochondrial fraction (P2), the bulk of the remaining activity being associated with the high-speed supernatant. Prolyl endopeptidase and cation-sensitive neutral endopeptidase thus can be regarded as mainly cytoplasmic enzymes in the rabbit brain.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D015260:Neprilysin; D009419:Nerve Tissue Proteins / Q000032:analysis; D000085822:Prolyl Oligopeptidases; D011817:Rabbits; D012697:Serine Endopeptidases; D013347:Subcellular Fractions / Q000201:enzymology
D009419:Nerve Tissue Proteins; D007770:L-Lactate Dehydrogenase; D002795:Choline O-Acetyltransferase; D010450:Endopeptidases; D012697:Serine Endopeptidases; D000085822:Prolyl Oligopeptidases; D015260:Neprilysin