title: Indirect fluorescent-antibody technique for serological diagnosis of La Crosse (California) virus infections.
abstract: A clinically relevant indirect fluorescent-antibody technique (IFA) was developed for the serological diagnosis of La Crosse virus infections. The IFA (67%) was as sensitive as the hemagglutination inhibition (58%) and neutralization (58%) tests in the detection of antibodies in acute-phase specimens. Immunoglobulin M antibodies were detected by the IFA test in 48% (11 of 23) of these specimens. Diagnostically significant increases in IFA titer were detected in 86% (19 of 22) of the paired samples. Antibodies were detectable in some patients 7 years after infection; however, the IFA test was not as sensitive as the other two tests in the detection of previous infections.
mesh_terms: D002648:Child; D002675:Child, Preschool; D004671:Encephalitis, Arbovirus / Q000175:diagnosis*; D004670:Encephalitis, California / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male; D009500:Neutralization Tests; D012698:Serologic Tests
chemical_list: D007075:Immunoglobulin M