Primary structure of an unusual glycine tRNA UGA suppressor.
We have determined the nucleotide sequences of two UGA-suppressing glycine transfer RNAs. The suppressor tRNAs were previously shown to translate both UGA and UGG and to have arisen as a consequence of mutation in glyT, the gene for the GGA/G-reading glycine tRNA of Escherichia coli. In each mutant tRNA, the primary sequence change was the substitution of adenine for cytosine in the 3' position of the anticodon. In addition, a portion of mutant glyT tRNA molecules contained N6-(delta 2-isopentenyl)-2-thiomethyl adenine adjacent to the 3' end of the anticodon (nucleotide 37). The presence or absence of this hypermodification may be a determinant in some of the biological properties of the mutant tRNA.
D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D009690:Nucleic Acid Conformation; D012346:RNA, Transfer, Amino Acyl; D013489:Suppression, Genetic
D012346:RNA, Transfer, Amino Acyl