Solid-phase immunosorbent haemadsorption (SPIHAd): a new, rapid and simple test for rubella-specific IgM.
A new, rapid and highly sensitive test for rubella-specific IgM not requiring pretreatment of serum for removal of non-specific inhibitors and heteroagglutinins was developed and compared with sucrose density gradient fractionation/haemagglutination inhibition. The test was shown to be highly sensitive in detecting rubella-specific IgM resulting from natural infection, but gave variable results in detection of rubella vaccine-induced IgM. The advantages of the solid-phase immunosorbent haemadsorption (SPIHAd) test are its rapidity, inexpensiveness, the large number of untreated whole sera that can be handled at one time, and the lack of interference by rheumatoid factor and by rubella-specific IgG.
D000914:Antibodies, Viral / Q000032:analysis*; D005189:False Positive Reactions; D006382:Hemadsorption; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D007163:Immunosorbent Techniques; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000276:immunology; D012217:Rheumatoid Factor; D012409:Rubella / Q000276:immunology; D012411:Rubella Vaccine / Q000276:immunology; D012412:Rubella virus / Q000276:immunology*
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D012411:Rubella Vaccine; D012217:Rheumatoid Factor