Comparison of indirect immunofluorescence and enzyme-linked immunosorbent assay for detection of toxoplasma antibody.
Eighty samples of human serum were tested for antibody to Toxoplasma gondii by enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence (IIF). Development of ELISA suggested that accurate results could only be obtained with serial serum dilutions. ELISA and IIF tests showed a significant degree of correlation, but ELISA was found to be more sensitive: 17% of 24 sera showing negative IIF values showed slightly positive ELISA titres.
D000906:Antibodies / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D014123:Toxoplasmosis / Q000276:immunology*
D000906:Antibodies