T cell responses to alloantigens. IV. In vivo and in vitro studies of the abrogation of presensitization to major histocompatibility antigens.
B6AF1 mice were sensitized to major histocompatibility complex (MHC) antigens by rejection of B10.D2 skin allografts. The effects of various protocols of treatment with antithymocyte serum (ATS), Bordetella pertussis vaccine, and donor strain lymphocytes were tested in these mice, and the results were assessed by in vitro testing for abrogation of circulating cytotoxic T cell memory and in vivo by determining the survival time of a second donor strain skin graft. It was found that a 6-week course of ATS alone abrogated this presensitized state as judged by both the in vitro and in vivo measurements, and that the hyporesponsive state induced by this treatment persisted for at least 4 months.
D000818:Animals; D000961:Antilymphocyte Serum / Q000276:immunology; D006085:Graft Survival; D006649:Histocompatibility Antigens / Q000276:immunology*; D007156:Immunologic Memory; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010567:Pertussis Vaccine / Q000276:immunology; D016038:Skin Transplantation; D013601:T-Lymphocytes / Q000276:immunology*
D000961:Antilymphocyte Serum; D006649:Histocompatibility Antigens; D010567:Pertussis Vaccine