title: Immunochemical analysis of anti-HLA-A2, HLA-A3, and HLA-B27 xenoantisera elicited with hybrids between human and murine cells.
abstract: Rabbits were immunized with hybrids constructed with human and murine cells. Serological and immunochemical studies showed  that xenoantiserum 1595 is operationally specific for HLA-A2, xenoantisera 0806 and 0746 for HLA-A3 and xenoantiserum 0745 for HLA-B27. The Fab2 blocking assay suggests a spatial relationship between allotypic determinants recognized by the xenoantisera and those reacting with the HLA-A, B-specific monoclonal antibodies (MoAb) Q1/28, 6/31, Q6/64, and CR-1 and the anti-beta 2-microglobulin (beta 2-mu) MoAb NAMB-1.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D004591:Electrophoresis, Polyacrylamide Gel; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology*; D007163:Immunosorbent Techniques; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D011817:Rabbits
chemical_list: D000906:Antibodies; D006680:HLA Antigens