Flow microcalorimetric bioassay of polyene antibiotics: interaction with growing Saccharomyces cerevisiae.
Microcalorimetric investigation of the interaction of polyene antibiotics with mid-exponential cells of a growing culture of Saccharomyces cerevisiae has been used as the basis of a bioassay procedure. The assay is rapid, sensitive and reproducible. The results are compared to classical assays and potency ranking orders.
D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D001681:Biological Assay / Q000379:methods; D002151:Calorimetry / Q000379:methods; D002174:Candicidin / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D008155:Lucensomycin / Q000494:pharmacology; D010866:Natamycin / Q000494:pharmacology; D009761:Nystatin / Q000494:pharmacology; D011090:Polyenes / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects*
D000935:Antifungal Agents; D011090:Polyenes; D009761:Nystatin; D002174:Candicidin; D008155:Lucensomycin; D000666:Amphotericin B; D010866:Natamycin