[Immobilization of antibodies against Australia antigen on nylon spheres: use in immunoenzymatic determination of HBsAg].
Antibodies against HBsAg were immobilized by covalent linkage on nylon beads to be used in solid phase enzyme-linked immunoassays. The covalent bond between the protein and the partially hydrolyzed support was obtained via glutaraldehyde or succinylester methods. These particular solid phases were compared, in a conventional enzyme-linked immunosorbent assay for hepatitis B antigen, with polystyrene beads coated by simple adsorption of IgG on plastic surface. The analytical response was more satisfactory when the polystyrene beads were used as immunoextractive support even if more IgG molecules could be immobilized on nylon beads.
D000914:Antibodies, Viral; D001666:Binding Sites, Antibody; D004797:Enzyme-Linked Immunosorbent Assay; D006509:Hepatitis B / Q000276:immunology*; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D007124:Immunoenzyme Techniques; D009757:Nylons
D000914:Antibodies, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D009757:Nylons
Immobilizazione di anticorpi anti-antigene Australia su sfere di nylon: utilizzazione in un dosaggio immunoenzimatico di HBsAg.