Surface immunoglobulins of tonsil lymphocytes.
Human tonsillar lymphocytes bound 14C-labelled homologous IgG, IgA, and IgM at 5 degrees C and released them at 35 degrees C. B cells were found to be more active in this type of Ig binding. The amount of lymphocytes which could be labelled with fluoresceinated anti-human Ig-s decreased during preincubation of the cells at 35 degrees C. Intrinsic Ig-s (determinants of lymphocytes) were demonstrated by immunofluorescent staining preceded by preincubation of the cells at 35 degrees C for 60 min. The ratios of IgG, IgA, and IgM bearing cells in the human tonsillar lymphocyte population were 16.0, 8.0 and 7.4% respectively. The weak binding of Ig-s to the surface of lymphocytes is discussed with regard to the demonstration of surface Ig determinants of lymphocytes.
D005455:Fluorescent Antibody Technique; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D008214:Lymphocytes / Q000276:immunology*; D014066:Palatine Tonsil; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D013696:Temperature
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell