An ELISA method for detecting unexpected antibodies.
This paper reports a new horseradish peroxidase enzyme linked immunosorbant assay (ELISA) to detect patient serum alloantibodies. The new methodology utilizes a water-soluble chromogen, O-dianisidine and a temporary solid antibody support, osmotically lysed reagent erythrocytes. In a double blind study the horseradish peroxidase ELISA was found to be as sensitive as the conventional antiglobulin test. In parallel dilution studies on serum samples containing anti-Kell antibodies, the horseradish peroxidase-ELISA was found to be substantially more sensitive than the conventional antiglobulin test. While the procedure is at present impractical for routine use, these results indicate a potential for improved sensitivity which warrants further research. Possible applications and prospective areas of research are discussed.
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D003298:Coombs Test; D003962:Dianisidine; D004311:Double-Blind Method; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology; D007518:Isoantibodies / Q000032:analysis*; D007626:Kell Blood-Group System / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D007518:Isoantibodies; D007626:Kell Blood-Group System; D003962:Dianisidine