Aniline blue-containing buffered charcoal-yeast extract medium for presumptive identification of Legionella species.
By utilizing buffered charcoal-yeast extract medium containing 0.01% aniline blue in conjunction with a long-wave UV light, the differentiation of five species of Legionella was facilitated. L. pneumophila, when grown on this medium, did not absorb the aniline blue dye; however, L. micdadei, L. dumoffii, L. bozemanii, and L. gormanii absorbed the dye in varying amounts and produced colonies of various shades of blue.
D000814:Aniline Compounds; D001431:Bacteriological Techniques; D002606:Charcoal; D003470:Culture Media; D005456:Fluorescent Dyes; D007875:Legionella / Q000145:classification* / Q000254:growth & development; D014466:Ultraviolet Rays; D015002:Yeast, Dried
D000814:Aniline Compounds; D003470:Culture Media; D005456:Fluorescent Dyes; D002606:Charcoal; C017006:aniline blue