title: Phage influence on the synthesis of extracellular toxins in group A streptococci.
abstract: Phage conversion of group A streptococci to produce streptococcal exotoxins was shown to occur more widely than has been previously reported. Toxigenic conversion was found in 19 newly constructed lysogenic and pseudolysogenic strains resulting in synthesis of exotoxin types A and B. Conversion was accomplished by a positive conversion effector, which was a phage characteristic expressed by the prophage and vegetatively reproducing phage. Exotoxin production was determined by the rabbit skin test and by countercurrent immunoelectrophoresis with type-specific antisera. New lysogens and pseudolysogens were constructed with strains which failed to produce at least one exotoxin type. Phages were obtained from toxigenic strains isolated from cases of scarlet fever. Conversions were consistent and repeatable; loss of the recently introduced phage was accompanied by loss of the newly acquired toxin productivity. Conversion resulted in production of additional exotoxin type or types and never affected existing toxin synthesis. Converting phages were characterized by electron microscopy and negatively stained preparations and were all found to be of morphological class B1. All phage nucleic acid was double-stranded DNA. Though similar in structure, each converting phage had a different host range, and the nine new converting phages identified here did not react with antiserum prepared against the originally reported converting phage.
mesh_terms: D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000145:classification; D001435:Bacteriophages / Q000502:physiology* / Q000648:ultrastructure; D005098:Exotoxins; D008242:Lysogeny; D008565:Membrane Proteins; D013297:Streptococcus pyogenes / Q000378:metabolism*; D014775:Virus Activation
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D005098:Exotoxins; D008565:Membrane Proteins; C117980:SpeA protein, Streptococcus pyogenes; C027951:erythrogenic toxin