Protein glycosylation in Trypanosoma cruzi. I. Characterization of dolichol-bound monosaccharides and oligosaccharides synthesized "in vivo".
Trypanosoma cruzi cells, the causative agent of Chagas disease, incubated with [U-14C]glucose in the presence of 5 mM sodium pyruvate or 5.5 mM glucose, were found to synthesize Man9GlcNAc2-P-P-dolichol as the main and largest dolichol disphosphate derivative. No traces of glucosylated derivatives or of dolichol-bound oligosaccharides with a size larger than that of a Man9GlcNAc2 standard could be detected in any case. The synthesis of mannose-P-dolichol but not that of glucose-P-dolichol was found to occur in vivo in these cells. The inability to synthesize glucose-P-dolichol may explain the absence of glucosylated dolichol diphosphate derivatives. The lipid moiety of the dolichol derivatives appeared to have about 13 isoprene residues, the first of which was saturated.
D000818:Animals; D005947:Glucose / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism; D009844:Oligosaccharides / Q000302:isolation & purification; D011103:Polyisoprenyl Phosphate Monosaccharides / Q000096:biosynthesis* / Q000302:isolation & purification; D011104:Polyisoprenyl Phosphate Oligosaccharides / Q000096:biosynthesis* / Q000302:isolation & purification; D011105:Polyisoprenyl Phosphate Sugars / Q000096:biosynthesis*; D014349:Trypanosoma cruzi / Q000378:metabolism*
D006023:Glycoproteins; D009844:Oligosaccharides; D011103:Polyisoprenyl Phosphate Monosaccharides; D011104:Polyisoprenyl Phosphate Oligosaccharides; D011105:Polyisoprenyl Phosphate Sugars; D005947:Glucose