Short- and long-term effects of dimethyl sulfoxide on mouse pancreatic islet B-cell function in vitro.
Dimethylsulfoxide has been used as a cryoprotectant for the endocrine pancreas. To explore possible harmful effects of Me2SO influenced neither glucose-stimulated insulin release, nor islet glucose oxidation. On the other hand, 1 M but not 0.25 M Me2SO decreased glucose-stimulated (pro)insulin and total protein biosynthesis in acute experiments. In islet culture experiments with Me2SO-supplemented culture media there was no obvious effects on glucose-stimulated (pro)insulin biosynthesis. It is suggested that Me2SO in the range 0.01-0.5 M does not affect islet B-cell function in vitro.
D000818:Animals; D003080:Cold Temperature; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D013997:Time Factors; D014021:Tissue Preservation
D003451:Cryoprotective Agents; D007328:Insulin; D005947:Glucose; D004121:Dimethyl Sulfoxide