[Advantages of spectrophotometric reading of ELISA plates for determination of the HBs/anti-HB system].
It has been tested some diluted HBsAg and anti-HBs positive sera by modified ELISA test. We have found a correlation between HBsAg titer and spectrophotometric reading value and we have shown it in a curve. On the other hand, by neutralization of different dilutions of a RIA anti-HBs positive serum with different dilutions of a HBsAg positive serum we have carried out a checkboard and we have found that the best titer for neutralizing HBsAg is 30 ng/ml. This HBsAg has been called "standard". Using HBsAg "standard" we have tested 50 sera of hemophiliacs and we have found 1 HBsAg positive (2%) and 38 anti-HBs positive patients (76%).
D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006509:Hepatitis B / Q000175:diagnosis; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006525:Hepatitis, Viral, Human / Q000175:diagnosis*; D006801:Humans; D007124:Immunoenzyme Techniques; D013053:Spectrophotometry
D000914:Antibodies, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens
Vantaggi della lettura spettrofotometrica di piastre ELISA per la determinazione del sistema HBs/anti-HBs.