title: Kinetical properties of the serum mannan-binding protein from rabbit. A comparison with those of the liver mannan-binding protein.
abstract: A serum lectin specific for mannose and N-acetylglucosamine residues (serum mannan-binding protein) was characterized as a glycine-rich protein of large molecular size. Binding of the lectin to 125I-labeled mannan was a reversible and saturable process with a dissociation constant of 2.3 X 10(-9) M and a maximum capacity of 3.6 pmol of 125I-labeled mannan per microgram of protein. alpha-Mannosidase, a lysosomal enzyme isolated from porcine kidney, bound to the lectin with high affinity (Kd = 2.8 X 10(-9) M). These kinetical parameters are compared with those of the liver mannan-binding protein in a discussion of the interrelationship of these two lectins.
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D002846:Chromatography, Affinity; D037222:Collectins; D007158:Immunologic Techniques; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D011817:Rabbits
chemical_list: D002352:Carrier Proteins; D037222:Collectins