[Rapid determination of the minimal inhibitory concentration of an antibiotic by measuring bacterial ATP (author's transl)].
The minimal inhibitory concentrations (MIC) of gentamicin for ten strains of Enterobacteriaceae were determined by measuring the bacterial ATP (adenosine triphosphate). ATP was measured by chemiluminescence on 4-h growth cultures which included gentamicin. The MIC results obtained by this method were equivalent to those found with the technique of dilution in liquid medium.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004754:Enterobacter / Q000187:drug effects; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects; D005839:Gentamicins / Q000494:pharmacology*; D007709:Klebsiella / Q000187:drug effects; D008163:Luminescent Measurements; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011511:Proteus / Q000187:drug effects; D012706:Serratia marcescens / Q000187:drug effects
D005839:Gentamicins; D000255:Adenosine Triphosphate
Détermination rapide de la concentration minimale inhibitrice d'un antibiotique par dosage de l'ATP bactérien.