Growth of human foreskin fibroblasts in a serum-free, defined medium without platelet-derived growth factor.
The hormones which support growth, in vitro, of normal, neonatal human foreskin fibroblasts were determined. Whereas thrombin and hydrocortisone were major growth stimulants, platelet-derived growth factor was not. Human foreskin fibroblasts grew in a serum-free, biochemically defined medium consisting of epidermal growth factor (100 ng/ml), insulin (100 ng/ml), transferrin 10 micrograms/ml), thrombin (1 microgram/ml), ascorbic acid (10 micrograms/ml), and hydrocortisone (5 x 10(-5) M) in a 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F-12, supplemented with ovalbumin (1 mg/ml) and trace elements. The growth achieved was comparable to that achieved with 5% fetal bovine serum. Neither platelet-derived growth factor, fibroblast growth factor, nor somatomedin activity increased proliferation. This serum-free medium, designated Defined Medium F, provides a biochemically defined system for growth and limited subcultivation of human foreskin fibroblasts in vitro.
D001792:Blood Platelets / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D005347:Fibroblasts / Q000502:physiology; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D007231:Infant, Newborn; D007328:Insulin / Q000494:pharmacology; D008297:Male; D010455:Peptides / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor; D012879:Skin Physiological Phenomena; D014131:Trace Elements / Q000494:pharmacology; D014168:Transferrin / Q000494:pharmacology
D003470:Culture Media; D006133:Growth Substances; D007328:Insulin; D010455:Peptides; D010982:Platelet-Derived Growth Factor; D014131:Trace Elements; D014168:Transferrin; D006854:Hydrocortisone