[Lysogenic conversion induced by phages phi 80. I. A description of the phenomenon and the cloning of the conversion gene].
Escherichia coli cells lysogenic for coliphage phi 80 stop adsorbing the superinfecting phi 80 phage after having been kept under anaerobic conditions for a long time, which conferred on these cells the TonA phenotype. To determine the location of the gene for lysogenic conversion (cor), BamHI fragments of phi 80 DNA were cloned in pBR322 plasmid. The cells transformed with the recombinant plasmid pDK01 = pBR322 + phi 80 BamHI fragment 1 immediately acquire the TonA phenotype. So, the cor gene(s) is contained in the central phi 80 BamHI fragment (fragment 1) which includes gene 13, the b2 region and the att site.
D000327:Adsorption; D002874:Chromosome Mapping; D003001:Cloning, Molecular / Q000379:methods*; D003090:Coliphages / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005785:Gene Conversion; D005814:Genes, Viral; D008242:Lysogeny; D010957:Plasmids
Lizogennaia konversiia, vyzyvaemaia fagom phi 80. Soobshchenie I. Opisanie iavleniia i klonirovanie gena konversii.