Smooth muscle myosin in precursor and mature smooth muscle cells in normal pulmonary arteries and the effect of hypoxia.
Exposure to hypoxia increases pulmonary arterial muscularity-in the intra-acinar arteries "new" muscle appears in the normally nonmuscular regions and in the preacinar arteries, medial thickness increases. In the present study by immunofluorescence techniques, the myosin content of the pulmonary arterial walls at two levels of the circulation (intra-acinar and preacinar) were studied in control rats and those exposed to hypobaric hypoxia of 380 torr for 3,7, 10, or 14 days. In control animals, we show that the precursor smooth muscle cells, pericytes and intermediate cells normally present in the nonmuscular regions of the intra-acinar arteries, contain smooth muscle myosin. With exposure to hypoxia, smooth muscle myosin in the intra-acinar arteries increases to Day 10, both in area of staining and fluorescent intensity. This is in contrast to the preacinar arteries were only the area of myosin increases. Antihuman platelet (non-muscle) myosin shows a little faint staining in both control and hypoxic animals. Adaptations to hypoxia by the intra-acinar precursor and preacinar mature smooth muscle cells is different, and suggests that the functions subserved by the myosin filaments at each of the two levels differs.
D000818:Animals; D005455:Fluorescent Antibody Technique; D000860:Hypoxia / Q000473:pathology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000032:analysis / Q000473:pathology*; D009218:Myosins / Q000032:analysis*; D011650:Pulmonary Alveoli / Q000473:pathology; D011651:Pulmonary Artery / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains
D009218:Myosins