Identification of the Salmonella typhimurium cysB gene product by two-dimensional protein electrophoresis.
Examination of two-dimensional electropherograms of proteins from wild-type Salmonella typhimurium and 16 different cysB strains permitted the identification of a single 34,500-dalton polypeptide chain with a pI of 7.6 that was the product of cysB. Exclusion chromatography indicated that the native cysB protein is a multimer of at least two and probably four or more such subunits.
D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D008970:Molecular Weight; D009154:Mutation; D012486:Salmonella typhimurium / Q000235:genetics*
D001426:Bacterial Proteins