Pre-embedding immunohistochemistry as an approach to high resolution antigen localization at the light microscopic level.
Pre-embedding immunohistochemistry with subsequent embedding in hydroxypropyl methacrylate enables one to obtain high resolution staining of antigens in 1 mu tissue sections. A routine method using formaldehyde fixation, methanol permeation, and an indirect method with fluorescein-labeled second antibody is described. This method is compared with other pre-embedding staining procedures. To illustrate the method the mouse small intestine was chosen as a model and stained with antibodies to tubulin, actin, and fibronectin. Some anticipated and some unusual staining patterns were found.
D000199:Actins / Q000032:analysis; D000818:Animals; D000941:Antigens / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D007421:Intestine, Small / Q000166:cytology; D051379:Mice; D008808:Mice, Inbred CBA; D009130:Muscle, Smooth / Q000166:cytology; D009197:Myenteric Plexus / Q000166:cytology
D000199:Actins; D000941:Antigens