[Repair and mutagenesis in Escherichia coli cells on induction in the DNA of monoadducts and cross-links by light-activated 8-methoxypsoralen. Dependence on the uvrA and polA genes].
Relative efficiency of (psoralen + near UV)-induced monoadducts and inter-strand cross-links of Escherichia coli cells has been studied, as well as the role of uvrA and polA genes in these processes. Inter-strand cross-links have been shown to be more effective, as compared with monoadducts both in cell inactivation and in mutagenesis. At least 3 ways for repair of DNA carrying monoadducts have been found, and only 2 for DNA carrying inter-strand cross-links, each of these being controlled by genes uvrA and polA in different modes.
D003434:Crossing Over, Genetic; D004260:DNA Repair / Q000187:drug effects*; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005798:Genes, Bacterial / Q000187:drug effects*; D008040:Genetic Linkage; D008730:Methoxsalen / Q000494:pharmacology* / Q000528:radiation effects; D009154:Mutation; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D008730:Methoxsalen
Reparatsiia i mutagenez v kletkakh Escherichia coli pri induktsii v DNK monoadduktov i poperechnykh sshivok aktivirovannym svetom 8-metoksipsoralenom. Zavisimost' ot genov uvrA i polA.