Specificity of tumor cell stimulation of DNA inhibitory cytokine production in vitro.
ST/a splenocytes and peritoneal macrophages sensitized to tumorigenic, isoimmunizing R+ ST-L cells in vivo are stimulated to produce a DNA inhibitory supernatant in vitro when co-cultured with R+ ST-L cells. Studying the cross-reactions with various uninfected and MuLV infected cells, evidence was obtained for the gp70 specificity of this stimulation. T-cell depletion by thymectomy and whole body irradiation caused a moderate reduction of the DNA inhibitory effect of the splenocyte culture supernatant. However, macrophage depletion by silicate caused an almost complete abolishment of DNA inhibitory cytokine production, which could not be restored by the addition of supplementary non-sensitized spleen cells.
D000818:Animals; D001688:Biological Products / Q000096:biosynthesis*; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D002478:Cells, Cultured; D003429:Cross Reactions; D016207:Cytokines; D004273:DNA, Neoplasm / Q000037:antagonists & inhibitors*; D005347:Fibroblasts / Q000276:immunology; D007111:Immunity, Cellular; D008168:Lung / Q000276:immunology; D008212:Lymphocyte Depletion; D008262:Macrophage Activation; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology / Q000473:pathology*; D013154:Spleen / Q000276:immunology; D013934:Thymectomy
D001688:Biological Products; D016207:Cytokines; D004273:DNA, Neoplasm