A light and electron microscopic histochemical study on the mechanism of DFP-induced acute and subacute myopathy.
The histochemical changes occurring in association with the development of acute and subacute myopathy have been studied in the rat diaphragm 30 min-48 h after a single i.p. injection of 1.82 mg/kg of the irreversible cholinesterase inhibitor organophosphate, diisopropylfluorophosphate (DFP). In addition to a considerable inhibition of the AChE activity of the motor end-plates, accumulation of ionic Ca2+ and an increase in neutral protease activity in the subjunctional sarcoplasm have been demonstrated. A temporal and causal relationship has been established between the histochemical changes and the development of the ultrastructural signs of myopathy.
D000208:Acute Disease; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002800:Cholinesterase Inhibitors; D003964:Diaphragm / Q000378:metabolism; D006651:Histocytochemistry; D007531:Isoflurophate / Q000494:pharmacology*; D008854:Microscopy, Electron; D009135:Muscular Diseases / Q000139:chemically induced / Q000473:pathology*; D010447:Peptide Hydrolases / Q000378:metabolism; D051381:Rats
D002800:Cholinesterase Inhibitors; D007531:Isoflurophate; D010447:Peptide Hydrolases; D002118:Calcium