title: An E coli microsuspension assay for the detection of DNA damage induced by direct-acting agents and promutagens.
abstract: We have devised a microsuspension assay utilizing E coli indicator strains WP2, WP2 uvrA, WP67, CM611, WP100, W3110polA+, and p3478 pola- for the detection of chemically-induced preferential kill of repair-deficient strains. Data are presented from tests of 77 compounds representing a wide range of chemical classes which demonstrate the efficiency of the E coli microsuspension assay as both a qualitative and quantitative screen of DNA-modifying activity. Furthermore, the use of a battery of indicator strains lacking different repair systems offers the advantage of providing preliminary information concerning the mechanism of DNA damage induction by a test agent.
mesh_terms: D000477:Alkylating Agents / Q000633:toxicity; D000588:Amines / Q000633:toxicity; D000818:Animals; D004269:DNA, Bacterial / Q000502:physiology*; D004926:Escherichia coli / Q000378:metabolism*; D006834:Hydrazines / Q000633:toxicity; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D009153:Mutagens / Q000494:pharmacology*; D009602:Nitrosamines / Q000633:toxicity; D011083:Polycyclic Compounds / Q000633:toxicity; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D000477:Alkylating Agents; D000588:Amines; D004269:DNA, Bacterial; D006834:Hydrazines; D009153:Mutagens; D009602:Nitrosamines; D011083:Polycyclic Compounds