[Comparison of in vitro methods for the determination of the activity of fosfomycin with the efficacy determined in experimentally infected mice (author's transl)].
Effective doses of fosfomycin against 17 strains of bacteria were determined in therapeutic experiments using infected mice. The peak serum levels obtained after administration of these doses were compared with the minimum inhibitory concentrations determined in vitro. Reasonable minimum inhibitory concentrations were obtained only with Mueller-Hinton agar containing glucose-6-phosphate: 1. Correlation of in vivo and in vitro efficacy is much better in comparison with the use of media without glucose-6-phosphate. 2. Minimum inhibitory concentrations below the levels obtained after therapeutic doses were found only in Mueller-Hinton agar containing glucose-6-phosphate. 3. Minimum inhibitory concentrations are better reproducible when determined on solid media than in broth.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001424:Bacterial Infections / Q000188:drug therapy*; D001431:Bacteriological Techniques; D003470:Culture Media; D005260:Female; D005578:Fosfomycin / Q000097:blood / Q000627:therapeutic use*; D005954:Glucosephosphate Dehydrogenase; D066298:In Vitro Techniques; D051379:Mice
D000900:Anti-Bacterial Agents; D003470:Culture Media; D005578:Fosfomycin; D005954:Glucosephosphate Dehydrogenase
Zur Methodik der Aktivitätsbestimmung von Fostomycin in vitro unter Berücksichtigung der Wirkung an der experimentell infizierten Maus.