Evaluation of the dye test, IgM-IFAT, and ELISA for the diagnosis of toxoplasmic infection in pregnancy.
This study was undertaken to determine, first the relationship between the Sabin-Feldman dye test and the enzyme-linked immunosorbent assay (ELISA) as far as the antitoxoplasmic IgG antibodies are concerned, and secondly the relationship between the indirect immunofluorescence test and the ELISA as regards the antitoxoplasmic IgM antibodies. The results achieved through this research show a good relationship between the dye test and IgG-ELISA, especially satisfactory with the low and medium values of the antibody titers. Furthermore, a very good agreement could be documented between IgM-ELISA and IgM-IFAT in the detection of IgM-specific antibodies in the early phases of toxoplasmic infection.
D004396:Coloring Agents; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008751:Methylene Blue; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis*; D011786:Quality Control; D014122:Toxoplasma / Q000032:analysis / Q000276:immunology; D014123:Toxoplasmosis / Q000175:diagnosis*
D004396:Coloring Agents; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008751:Methylene Blue