Purification of horse eosinophil peroxidase.
Eosinophil peroxidase (donor: hydrogen-peroxidase oxidoreductase, EC 1.11.1.7) was isolated in a highly purified form (415/280 nm ratio, 1.05) from horse peripheral blood eosinophil. Eosinophil peroxidase was extracted from intact eosinophils (98-100% purity) or isolated eosinophil granules with 0.05 M acetate buffer (pH 4.7)/0.18 M NaCl and purified by chromatography on Sephadex G-200 and carboxymethylcellulose. Final elution was with 0.05 M acetate buffer (pH 4.7)/ 1 M NaCl. Horse eosinophil peroxidase is a strongly basic protein with bacterial properties when combined with H2O2 and iodide, bromide or, to a lesser degree, chloride.
D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange; D003594:Cytoplasmic Granules / Q000201:enzymology; D004804:Eosinophils / Q000201:enzymology*; D004926:Escherichia coli / Q000187:drug effects; D006736:Horses / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D010544:Peroxidases / Q000097:blood / Q000302:isolation & purification* / Q000494:pharmacology; D013057:Spectrum Analysis
D010544:Peroxidases