[Nephelometric inhibition immunoassay (NIIA), a new methodology for the determination of phenobarbital and phenytoin concentration in serum (author's transl)].
Phenobarbital and phenytoin concentration was determined in serum by the aid of the nephelometric inhibition immunoassay (NIIA) and the homogeneous enzyme immunoassay (EMIT). Both methods showed a good precision and accuracy. The comparison of the two methods in 77 sera for phenobarbital (3--68 micrograms/ml) and 39 sera for phenytoin (1.3--44 micrograms/ml) showed a very close correlation. The correlation coefficient for phenobarbital was 0,9525 and for phenytoin 0,9714. The special merits of the NIIA-method were precision, less of interference and low costs in reagent material.
D006801:Humans; D007118:Immunoassay; D007124:Immunoenzyme Techniques; D009391:Nephelometry and Turbidimetry; D010634:Phenobarbital / Q000097:blood*; D010672:Phenytoin / Q000097:blood*
D010672:Phenytoin; D010634:Phenobarbital
Nephelometrischer Inhibitionsimmunoassay (NIIA), ein neues Verfahren zur Bestimmung der Phenobarbital- und Phenytoin-Konzentration im Serum. Vergleich mit der Enzymimmunoassay-Methode (EMIT).