title: Immunohistochemical evidence of indolamine neurons in monkey spinal cord.
abstract: In this study, we have identified over 150 cells in the adult macaque spinal cord that are immunoreactive when stained with an antibody to 5-HT by means of the PAP method. The staining was blocked by preabsorption of the primary antibody by the 5-HT-BSA conjugate (used to generate the antibody) and by 5-HT, while BSA, dopamine, norepinephrine, 5-methoxytryptamine and tryptamine preabsorption did not block staining. In addition, the Falck-Hillarp histofluorescence technique was applied to the lumbosacral cord from one of the three monkeys studied; results confirm the presence of cells with yellow fluorescence and rapid fading to brown characteristic for serotoninergic cells. The neuronal identity of these immunoreactive cells is based on light and electron microscopic morphology and the presence of synaptic terminals in contact with labeled somata and dendrites. Most of these neurons were small (10-25 micrometers) and located ventral to the central canal in lamina X. Some processes of the cells extended into the intermediate gray and the ventromedial area of the ventral horn; other processes wrapped around large blood vessels in lamina X or around the wall of the central canal. Cells were most frequent in the cervical cord (approximately 6-7/mm length of cord) and less frequent in the thoracic (1.5/mm), lumbar (3/mm), and sacral (2/mm) cord. A few cells were also found in the marginal and gelatinosa regions of the dorsal horn of the sacral cord. Examination of sections from the medulla-spinal cord junction (obex level) indicates that the spinal cells may be an extension of cell groups located near the raphe obscurus in the gray region around the IVth ventricle. The indolamine spinal cells may act as interneurons in spinal circuits, control spinal blood flow through vessel innervation, or play a role in CSF composition through central canal innervation.
mesh_terms: D000818:Animals; D002552:Cerebral Ventricles / Q000378:metabolism; D007124:Immunoenzyme Techniques; D008251:Macaca; D008526:Medulla Oblongata / Q000378:metabolism; D008854:Microscopy, Electron; D009434:Neural Pathways / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D012701:Serotonin / Q000378:metabolism*; D013116:Spinal Cord / Q000033:anatomy & histology / Q000378:metabolism*
chemical_list: D012701:Serotonin