In vitro proliferative response and polyclonal antibody production in spleen cells of immunologically defective CBA/N and C3H/HeJ mice by water-soluble adjuvant (Bu-WSA) extracted from Bacterionema matruchotii.
Mitogenicity and the polyclonal plaque forming cell (PFC)-inducing property of a water soluble-adjuvant extracted from Bacterionema matruchotii by butanol (Bu-WSA) were examined in vitro in the spleen cells of hybrid (CBA/N female X BALB/c male)F1 mice and C3H strain of mice. The hybrid F1 male cells which expressed a CBA/N-defect were unable to respond to Bu-WSA, when assessed by the incorporation of [3H]thymidine into the cells and the generation of anti-trinitrophenyl (TNP)-PFC  or autoantibody PFC defined by the anti-bromelain-treated mouse erythrocyte PFC assay. However, hybrid F1 female cells with normal traits responded to Bu-WSA. Cultured spleen cells of bacterial lipopolysaccharide (LPS)-nonresponsive (C3H/HeJ mice responded to Bu-WSA as in the case of cells of LPS-responsive C3H/He mice, and the [3H]thymidine-uptakes and the numbers of PFC in these culture cells increased. Re-extraction of Bu-WSA by phenol did not affect its activities, while the activity of butanol-extracted LPS and C3H/HeJ cells decreased after re-extraction by the same procedure with phenol.
D000191:Actinomycetaceae / Q000276:immunology; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000276:immunology; D001323:Autoantibodies / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D006462:Hemolytic Plaque Technique; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D011135:Polysaccharides, Bacterial / Q000494:pharmacology; D013154:Spleen / Q000276:immunology
D000276:Adjuvants, Immunologic; D001323:Autoantibodies; D011135:Polysaccharides, Bacterial