Binding of parbendazole to tubulin and its influence on microtubules in tissue-culture cells as revealed by immunofluorescence microscopy.
We have shown that the benzimidazole carbamate, parbendazole, is a potent inhibitor of microtubule assembly in vitro and in vivo. Radiolabelled parbendazole was shown to bind to purified tubulin. Immunofluorescence studies using antitubulin antibody showed that parbendazole effectively depolymerizes cytoplasmic microtubules in animal cells leaving only one or two microtubules associated with one centriole. The usefulness of parbendazole and other benzimidazole carbamates as inhibitors of microtubule functions is discussed.
D000818:Animals; D001562:Benzimidazoles / Q000378:metabolism* / Q000494:pharmacology; D001665:Binding Sites; D002460:Cell Line; D002522:Chlorocebus aethiops; D003864:Depression, Chemical; D005455:Fluorescent Antibody Technique; D007700:Kinetics; D008870:Microtubules / Q000187:drug effects* / Q000648:ultrastructure; D014404:Tubulin / Q000378:metabolism*
D001562:Benzimidazoles; D014404:Tubulin; C100292:parbendazole