title: Escherichia coli mutant which restricts T7 bacteriophage has an altered RNA polymerase.
abstract: We have previously described an Escherichia coli K-12 mutant, Y49, which restricts the growth of bacteriophage T7 and causes the accumulation of short DNA molecules and head-related particles during infection. We now show that the basis for these effects is the inability of the T7 gene 2 product to inactivate the Y49 RNA polymerase during infection, similar to what has been shown by DeWyngaert and Hinkle (J. Biol. Chem. 254:11247--11253, 1979) for the BR3 and tsnB strains of E. coli.
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D009154:Mutation; D013604:T-Phages / Q000254:growth & development* / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases