Morphological destruction of cultured cells by the attachment of Treponema pallidum.
The incubation of Treponema pallidum with rabbit testicular cells, HEP-2 cells, human foreskin cells, rat cardiac cells, and rat skeletal muscle cells caused morphological disruption of these cultured cells. Control preparations of heat-inactivated treponemes, a high-speed supernatant in which treponemes had been pelleted, and culture medium failed to damage the tissue cells, as did viable treponemes when the cells were incubated in inverted Sykes-Moore chambers. Thus, cellular disruption is not associated with soluble treponemal, soluble inflammatory, or soluble testicular constituents but is mediated by the specific attachment of T pallidum. This organism apparently elaborates some type of toxic activity that lyses membranes: this may explain some of the histopathology of syphilitic disease.
D000818:Animals; D002462:Cell Membrane / Q000382:microbiology; D002478:Cells, Cultured / Q000382:microbiology* / Q000648:ultrastructure; D006321:Heart / Q000382:microbiology; D006358:Hot Temperature; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D009132:Muscles / Q000382:microbiology / Q000648:ultrastructure; D010413:Penis / Q000382:microbiology; D011817:Rabbits; D051381:Rats; D013737:Testis / Q000382:microbiology / Q000648:ultrastructure; D014210:Treponema pallidum / Q000648:ultrastructure