The effects of storage media and perfusion on osteoblast and osteocyte survival in free composite bone grafts.
Twenty-two adult mongrel dogs were used to investigate the effects of storage alone and storage and perfusion in 3 different storage media on the survival of osteocytes and osteoblasts in free bone grafts revascularized by microvascular anastomoses. Evaluation of the grafts at 2 weeks demonstrated that storage in chilled (+ 5 degrees C) physiologic saline or Collins-Terasaki solution resulted in greater survival of osteocytes and osteoblasts than did storage in chilled BGJb solution or in room temperature air. No beneficial effect could be detected from perfusing the bone grafts with their storage media. On the contrary, deleterious effects were noted at the sites of the anastomoses, with thromboses of vessels secondary to endothelial damage.
D000818:Animals; D001158:Arteries / Q000601:surgery; D016025:Bone Transplantation; D003470:Culture Media; D004285:Dogs; D006085:Graft Survival; D007264:Inguinal Canal / Q000098:blood supply; D007552:Isotonic Solutions / Q000627:therapeutic use; D008866:Microsurgery; D009333:Neck / Q000098:blood supply; D009930:Organic Chemicals; D010006:Osteoblasts / Q000502:physiology*; D010011:Osteocytes / Q000502:physiology*; D010477:Perfusion; D012272:Ribs / Q000098:blood supply; D012462:Saline Solution, Hypertonic; D014021:Tissue Preservation / Q000379:methods*; D014656:Vascular Surgical Procedures; D014680:Veins / Q000601:surgery
C022788:BGJ culture medium; C033004:Collins-Terasaki solution; D003470:Culture Media; D007552:Isotonic Solutions; D009930:Organic Chemicals; D012462:Saline Solution, Hypertonic