title: Protein synthesis using poly(2'-halogeno-2'-deoxyadenylic acids) as messenger.
abstract: Poly(2'-fluoro-2'-deoxyadenylic acid), poly(2'-chloro-2'-deoxyadenylic acid) and poly(2'-bromo-2'-deoxyadenylic acid) are used as messenger RNAs in protein synthesizing systems in vitro. All polynucleotides were active as messengers and [14 C]lysine was incorporated into polypeptides. The initial velocity of polylysine formation was greater using poly(2'-fluoro-2'-deoxyadenylic acid) as messenger than in the case of poly(rA), and all synthetic messengers lived longer in the protein synthetic system.
mesh_terms: D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D011061:Poly A / Q000235:genetics; D011089:Polydeoxyribonucleotides; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics; D013329:Structure-Activity Relationship
chemical_list: D011089:Polydeoxyribonucleotides; D012333:RNA, Messenger; D011061:Poly A; C035306:poly(2'-chloro-2'-deoxyadenylic acid); C035307:poly(2'-bromo-2'-deoxyadenylic acid); C020473:poly(2'-fluoro-2'-deoxyadenylic acid); D012343:RNA, Transfer