Protease-insensitive sea urchin embryo cell adhesions become protease sensitive in the presence of azide or cytochalasin B.
To understand the nature of the cell adhesions that must be modified during sea urchin embryo primary mesenchyme formation, we are studying the adhesive components of the hatched blastula stage embryo of Strongylocentrotus purpuratus. Pronase treatment conditions have been defined that leave the cells intact and able to recover from the effects of the protease upon its removal. Under these conditions, adhesion of the cells to tissue culture plates is totally eliminated, but cell-cell adhesion formation is only partially inhibited. Analysis of iodinated cell surface proteins indicates that most are affected by the pronase. Further studies of pronase effects found that sodium azide-treated cells are slightly adhesive and that pronase treatment of azide-treated cells totally eliminates cell-cell adhesions.
D000818:Animals; D001386:Azides / Q000494:pharmacology*; D001755:Blastocyst / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects*; D002462:Cell Membrane / Q000187:drug effects; D003571:Cytochalasin B / Q000494:pharmacology*; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D008565:Membrane Proteins / Q000378:metabolism; D010447:Peptide Hydrolases / Q000494:pharmacology*; D012617:Sea Urchins
D001386:Azides; D008565:Membrane Proteins; D003571:Cytochalasin B; D010447:Peptide Hydrolases