[Karyo- and cytometric investigations of cortical layer V pyramidal neurons of albino rat after three various fixations].
Layer V-pyramidal neurons of the temporal neocortex of albino rat (24 and 36 days old) were examined morphometricly. Length and width of the perikaryon, long and short diameter of the nucleus and the position of the nucleus in the perikaryon were compared with each other after three fixations (Formalin, Bouin, Carnoy). Changes of form and/or position caused by fixation allow no exact statements about the condition in vivo. They are founded on the changes on molecular plain. Resultant problems for karyometric investigations are pointed out.
D000085:Acetates / Q000494:pharmacology; D019342:Acetic Acid; D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D002540:Cerebral Cortex / Q000166:cytology*; D002725:Chloroform / Q000494:pharmacology; D003584:Cytological Techniques; D000431:Ethanol / Q000494:pharmacology; D005404:Fixatives / Q000494:pharmacology*; D005557:Formaldehyde / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000648:ultrastructure; D010851:Picrates / Q000494:pharmacology; D051381:Rats; D013702:Temporal Lobe
D000085:Acetates; C033474:Carnoy's solution; D005404:Fixatives; D010851:Picrates; D005557:Formaldehyde; C026239:Bouin's solution; D000431:Ethanol; D002725:Chloroform; D019342:Acetic Acid
Karyo- und zytometrische Untersuchungen an corticalen Lamina V-Pyramidenzellen der Albinoratte unter verschiedenen Fixierungsbedingungen.