Expression of the L11-L1 operon in mutants of Escherichia coli lacking the ribosomal proteins L1 or L11.
Using a variety of immunological techniques, the supernatant levels of ribosomal proteins were measured in mutants lacking the ribosomal proteins L1 or L11, and in wild-type strains. There was a 2.5--5-fold elevation of protein L11 level in the supernatant of strains lacking protein L1, compared to wild-type. In contrast, there was no elevation, but rather a diminution, in the corresponding L1 level in strains lacking protein L11, compared to wild-type. These results are consistent with a model for the control of expression of the L11-L1 operon in which protein L1 is an inhibitor of expression of that operon, but protein L11 is not. The supernatant concentrations of other proteins were indistinguishable in all strains.
D001426:Bacterial Proteins / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005246:Feedback; D005786:Gene Expression Regulation; D007122:Immunoelectrophoresis; D009154:Mutation; D009876:Operon; D012269:Ribosomal Proteins / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D012269:Ribosomal Proteins; C028546:ribosomal protein L1; C027624:ribosomal protein L11