Mice with the xid defect have helper cells for T15 idiotype-dominant anti-phosphorylcholine primary and secondary plaque-forming cells responses.
We have examined the abilities of helper T cells from commercially available (CBA/N X BALB/c)F1 (NBF1) xid male and phenotypically normal female mice to help T15+ and T15- B cells to produce thymus-dependent phosphorylcholine (PC)-specific direct plaque-forming cell responses. Carrier-primed T cells from both male and female mice were found (a) to restore T15+ TD responses in congenitally athymic BALB/c mice, (b) to help PC-primed BALB/c splenic B cells produce predominantly T15+ responses, and (c) to provide help for T15+ and T15- PFC responses generated by PC-primed normal F1 splenic B cells. Furthermore, carrier-primed irradiated xid and normal recipients contributed adequate helper activity for T15 dominant responses. We therefore conclude that male and female NBF1 mice are equally capable of helping T15+ responses.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D002794:Choline / Q000031:analogs & derivatives*; D005260:Female; D006462:Hemolytic Plaque Technique; D007114:Immunization; D007117:Immunization, Secondary; D007130:Immunoglobulin Idiotypes / Q000235:genetics* / Q000276:immunology; D007153:Immunologic Deficiency Syndromes / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D010767:Phosphorylcholine / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D007130:Immunoglobulin Idiotypes; D010767:Phosphorylcholine; D002794:Choline