Isolation and mapping of a uracil-sensitive mutant of Salmonella typhimurium.
A uracil-sensitive mutant of Salmonella typhimurium was isolated by diethyl sulfate mutagenesis and penicillin counterselection. This mutation identifies a new Salmonella gene that is well separated from the structural genes for arginine and pyrimidine biosynthesis. The use-1 mutation was located between the ilv gene cluster (isoleucine-valine operon) and hisR (structural gene for histidine tRNA) at 83 map units.
D003433:Crosses, Genetic; D005796:Genes / Q000187:drug effects; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D013463:Sulfuric Acid Esters / Q000494:pharmacology; D014161:Transduction, Genetic; D014498:Uracil / Q000378:metabolism*
D009153:Mutagens; D013463:Sulfuric Acid Esters; D014498:Uracil; C011612:diethyl sulfate