A method for rapid determination of IgG containing circulating immune complexes using polyethyleneglycol and radioactively labeled protein A.
A simple method for rapid determination of IgG containing circulating immune complexes by commercially available reagents was developed. In this method, serum is incubated with 2.5% polyethyleneglycol. After washing, the precipitate is incubated with radioactively labeled protein A, which binds to IgG in the immune complex. After a further washing, the radioactivity bound is measured. Artificially formed complexes of heat-aggregated human IgG are diluted and a reference curve is constructed. This method is compared to the solid phase C1q-binding method. Our method is slightly more sensitive than the C1q-binding method.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000936:Antigen-Antibody Complex / Q000032:analysis*; D002648:Child; D003166:Complement Activating Enzymes; D015922:Complement C1q; D005260:Female; D006801:Humans; D007074:Immunoglobulin G; D007158:Immunologic Techniques; D008297:Male; D008875:Middle Aged; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013205:Staphylococcal Protein A / Q000494:pharmacology*; D013997:Time Factors
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D011092:Polyethylene Glycols; D015922:Complement C1q; D003166:Complement Activating Enzymes