A comparison of indirect immunofluorescence and electron microscopy for the diagnosis of some haemorrhagic viruses in cell cultures.
Yellow fever, dengue (types 1, 2 and 4), Chikungunya, Rift Valley fever, Ebola, Marburg, and Lassa viruses were inoculated into susceptible cell cultures and daily investigated by indirect immunofluorescence (IFA) and electron microscopy (EM) with a view to achieve an early detection-identification of these agents. Compared to the other cell lines tested (Vero, BHK-21 and Aedes albopictus), CV-1 cells were found to be more sensitive. Viral antigens were detected by IFA from a few hours post inoculation (CHIK and RVF) to a maximum of 3 days (YF and EBO). For most of the viruses studied, the cytopathic effect (CPE) commenced 2-3 days after the detection of viral antigens. Virus particles were detected by EM only in the case of EBO, MBG and LAS, before any CPE was observed in cell cultures.
D000818:Animals; D001116:Arenaviridae / Q000302:isolation & purification*; D002043:Bunyaviridae / Q000302:isolation & purification*; D002460:Cell Line; D002646:Chikungunya virus / Q000302:isolation & purification; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D003716:Dengue Virus / Q000302:isolation & purification; D005455:Fluorescent Antibody Technique; D007836:Lassa virus / Q000302:isolation & purification*; D008722:Methods; D008854:Microscopy, Electron; D012209:Rhabdoviridae / Q000302:isolation & purification*; D012296:Rift Valley fever virus / Q000302:isolation & purification*; D014035:Togaviridae / Q000302:isolation & purification*; D015005:Yellow fever virus / Q000302:isolation & purification