The assessment of DNA-synthetic activity.
A method is described by which a numerical value can be assigned to the amount of DNA-synthesis shown graphically by population-histograms obtained by Feulgen cytophotometry. The index appeared to give a reasonable measure of DNA-synthetic activity in populations having very low, moderate and high mitotic indices and closely followed labelling indices obtained by autoradiography. Thus the advantage of a numerical value is that the DNA-synthetic activities in different populations can be compared for statistical analysis.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004396:Coloring Agents; D003584:Cytological Techniques; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication; D006168:Guinea Pigs; D008099:Liver / Q000378:metabolism*; D008940:Mitotic Index; D051381:Rats; D012394:Rosaniline Dyes; D012867:Skin / Q000378:metabolism*
D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D004247:DNA