title: Immunohistochemical demonstration of a testicular substance related to luteinizing hormone-releasing hormone.
abstract: A substance related to luteinizing hormone-releasing hormone was demonstrated, by immunohistochemical procedures, in the cytoplasm of interstitial cells within the rat testes. In many seminiferous tubules, nuclei of spermatogonial cells were also immunopositive. Both cytoplasmic and nuclear fractions of testicular homogenates contain immunoreactive compounds, and this report identifies which cell types contain this substance. The localization of a peptide hormone within the nucleus of a target cell population may indicate its mode of action.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007158:Immunologic Techniques; D007985:Leydig Cells / Q000378:metabolism; D008297:Male; D051381:Rats; D013093:Spermatogonia / Q000378:metabolism; D013737:Testis / Q000378:metabolism*
chemical_list: D007987:Gonadotropin-Releasing Hormone