title: Lipid on capsular polysaccharides of gram-negative bacteria.
abstract: Hydrolysis of the meningococcal group A, B, and C, and Escherichia coli K92 polysaccharides by 60% aqueous hydrofluoric acid liberated various 1,2-diacylglycerols. These were extracted with chloroform, trimethylsilylated, and analyzed by gas chromatography-mass spectrometry. Two 1,2-diacylglycerols were the major components isolated. In each polysaccharide, 80 to 90% dipalmitoyl glycerol  and 10 to 20% distearoyl glycerol were identified. No monoacylglycerols or mixed diacylglycerols were noted. The presence of the hydrophobic end causes the polysaccharides to aggregate in a micellar form and may be the entity by which the polysaccharide remains attached to the outer membrane of the bacterium giving rise to the structure recognized as a capsule.
mesh_terms: D004075:Diglycerides / Q000032:analysis*; D004926:Escherichia coli / Q000032:analysis*; D005989:Glycerides / Q000032:analysis*; D008070:Lipopolysaccharides / Q000032:analysis*; D013058:Mass Spectrometry; D011135:Polysaccharides, Bacterial / Q000032:analysis*
chemical_list: D004075:Diglycerides; D005989:Glycerides; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial