Role of the constant uridine in binding of yeast tRNAPhe anticodon arm to 30S ribosomes.
Twenty-two anticodon arm analogues were prepared by joining different tetra, penta, and hexaribonucleotides to a nine nucleotide fragment of yeast tRNAPhe with T4 RNA ligase. The oligomer with the same sequence as the anticodon arm of tRNAPhe bind poly U programmed 30S ribosomes with affinity similar to intact tRNAPhe. Analogues with an additional nucleotide in the loop bind ribosomes with a weaker affinity whereas analogues with one less nucleotide in the loop do not bind ribosomes at all. Reasonably tight binding of anticodon arms with different nucleotides on the 5' side of the anticodon suggest that positions 32 and 33 in the tRNAPhe sequence are not essential for ribosome binding. However, differences in the binding constants for anticodon arms containing modified uridine residues in the "constant uridine" position suggest that both of the internal "U turn" hydrogen bonds predicted by the X-ray crystal structure are necessary for maximal ribosome binding.
D000926:Anticodon / Q000378:metabolism*; D007700:Kinetics; D009843:Oligoribonucleotides / Q000378:metabolism; D011130:RNA Ligase (ATP); D012343:RNA, Transfer / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae; D013329:Structure-Activity Relationship; D013604:T-Phages / Q000201:enzymology; D014529:Uridine
D000926:Anticodon; D009843:Oligoribonucleotides; D012346:RNA, Transfer, Amino Acyl; D012343:RNA, Transfer; D011130:RNA Ligase (ATP); D014529:Uridine