Determination of LDL receptors on cultured lymphocytes using fluorescein-labeled LDL.
A rapid, sensitive procedure for the detection of LDL receptors on cell surface without using radioisotopes is described. Fluorescein-isothiocyanate (FITC) labeled LDL bound with cultured human lymphocytes, and the degree of that binding was compatible with that of 125 I-labeled LDL. The staining of cells with FITC-LDL allows identification and enumeration of LDL-binding cells using fluorescence microscopy or a fluorescence activated cell sorter (FACS). It is suggested that FITC-LDL can be applied for screening of patients with various degrees of activity in LDL receptors on their cell surfaces.
D000328:Adult; D001666:Binding Sites, Antibody; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008875:Middle Aged
D008077:Lipoproteins, LDL