Rapid analysis of immunoglobulin isoelectric focusing patterns with cellulose nitrate sheets and immunoperoxidase staining.
Immunoglobulins which have been focused to their isoelectric points in thin-layer polyacrylamide gels can be transferred by diffusion to the surface of cellulose nitrate sheets with retention of the original focusing patterns. IgG can be detected with peroxidase-linked antiserum. Unlike polyacrylamide gel, cellulose nitrate offers no permeability barrier, and so washing times are reduced from days to minutes. Thus small quantities (less than 1 microgram) of IgG may be visualized within 24 h of isoelectric focusing. In addition after immobilization on cellulose nitrate, immunoglobulin reactivity with antigens, including those of high molecular weight, may be analysed.
D000818:Animals; D000918:Antibody Specificity; D002482:Cellulose / Q000031:analogs & derivatives; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid; D007075:Immunoglobulin M; D007136:Immunoglobulins; D007525:Isoelectric Focusing / Q000379:methods*; D009103:Multiple Sclerosis / Q000175:diagnosis; D011817:Rabbits
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D002482:Cellulose