Immunochromatographic purification of Bean Yellow Mosaic Virus.
The method of immunoadsorptional purification of Bean Yellow Mosaic Virus has been worked out. Immunosorbents were obtained by coupling the antibody (IgG) fraction isolated from anti-BYMV and anti-pea leaf protein antisera with CNBr-activated 1% agarose beads. Conditions for preparation of immunosorbents, for BYMV adsorption and elution as well as the method of plant protein separation from BYMV were pointed out. The purity of BYMV was checked by double immunodiffusion as well as by SDS-acrylamide gel electrophoresis. Also biological activity was determined. TMV was used as the model virus for further BYMV studies.
D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000382:microbiology; D007163:Immunosorbent Techniques; D007164:Immunosorbents / Q000494:pharmacology; D009029:Mosaic Viruses / Q000302:isolation & purification*; D010940:Plant Proteins / Q000032:analysis; D010946:Plants, Medicinal
D007164:Immunosorbents; D010940:Plant Proteins