[Preliminary serological analysis for identification of direct monoclonal antibodies against surface structures of HLA typed human lymphocytes].
The following schedule has been applied to the selection and preliminary serological definition of monoclonal antibodies against HLA controlled antigenic structures of human lymphocyte surface: 1) identification of antibodies reactive against the immunizing cells by means of indirect radiobinding technique and complement-dependent lymphocytotoxicity; 2) analysis of the pattern of reaction against a panel of T and B lymphocytes purified from peripheral blood of HLA typed donors. The reaction has been performed also against lymphocytes pretreated with anti-human-beta 2 microglobulin antibodies. Thus monomorphic and polymorphic monoclonal antibodies, directed against beta 2 microglobulin associated or not associated antigens, can be identified. The importance of the complement pool and of antibody avidity in the lymphocytotoxicity reaction mediated by monoclonal antibodies, is evaluated.
D000906:Antibodies / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000961:Antilymphocyte Serum; D002462:Cell Membrane / Q000276:immunology*; D006680:HLA Antigens; D006650:Histocompatibility Testing; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D011863:Radioimmunoassay; D013601:T-Lymphocytes / Q000276:immunology; D001613:beta 2-Microglobulin / Q000276:immunology
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000961:Antilymphocyte Serum; D006680:HLA Antigens; D001613:beta 2-Microglobulin
Analisi sierologica preliminare per l'identificazione di anticorpi monoclonali diretti contro strutture della superficie dei linfociti umani codificate dal sistema HLA.