Mapping of neuronal contacts with intracellular injection of horseradish peroxidase and Lucifer yellow in combination.
A technique for the simultaneous visualization in the light microscope of processes of neurons filled with horseradish peroxidase and Lucifer yellow in combination has been developed. The technique is applied to determine the location, number and distribution of presumptive synaptic sites between neurons in the leech central nervous system.
D000818:Animals; D001931:Brain Mapping / Q000379:methods*; D003712:Dendrites / Q000648:ultrastructure; D005456:Fluorescent Dyes; D005724:Ganglia / Q000648:ultrastructure; D006652:Histological Techniques; D006735:Horseradish Peroxidase; D007865:Leeches; D008465:Mechanoreceptors / Q000648:ultrastructure; D008856:Microscopy, Fluorescence; D009046:Motor Neurons / Q000648:ultrastructure; D009433:Neural Inhibition; D009474:Neurons / Q000648:ultrastructure*; D013569:Synapses / Q000648:ultrastructure*
D005456:Fluorescent Dyes; D006735:Horseradish Peroxidase