Gel electrophoresis-derived enzyme-linked immunosorbent assay of serum from cows resistant to and cows susceptible to challenge exposure with Mycoplasma bovis.
The gel electrophoresis-derived enzyme-linked immunosorbent assay (GED-ELISA) technique combines the high resolving power of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to separate complex molecules by their molecular weights, with the high sensitivity of the ELISA to detect specific antibody, Sera from 4 cows that demonstrated resistance to challenge exposure and 4 cows that were susceptible to challenge exposure with live virulent Mycoplasma bovis strain 201 were subjected to GED-ELISA to determine reactivity with M bovis antigenic components separated by SDS-PAGE. The GED-ELISA mean reactivity of sera from the 2 groups did not differ significantly (P = 0.17) when subjected to analysis of variance. Sera from both groups recognized distinct fractions of M bovis.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002417:Cattle / Q000276:immunology*; D002418:Cattle Diseases / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D009174:Mycoplasma / Q000276:immunology*; D009175:Mycoplasma Infections / Q000276:immunology / Q000662:veterinary
D000907:Antibodies, Bacterial; D000949:Histocompatibility Antigens Class II; D007074:Immunoglobulin G