Molecular organization of heat-labile enterotoxin genes originating in Escherichia coli of human origin and construction of heat-labile toxoid-producing strains.
Recombinant deoxyribonucleic acid technology was employed to construct heat-labile enterotoxin (LT) toxoids. A recombinant plasmid carrying both an LT promoter region and LT subunit A (LTA) gene, lacking as much as 0.25 kilobases of the region up to the C terminus, produced a peptide possessing immunological properties of LTA but lacking the ability to construct LT activity (designated as LTA*). A cloned LT subunit B (LTB) gene produced LTB when a promoter on a vector was available for the gene. Escherichia coli producing LTA* and LTB (LT toxoids) could be useful as a vaccine.
D001427:Bacterial Toxins; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004768:Enterotoxins / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D010957:Plasmids; D014121:Toxoids / Q000096:biosynthesis / Q000235:genetics*
D001427:Bacterial Toxins; D004274:DNA, Recombinant; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D014121:Toxoids; C027538:heat-labile enterotoxin, E coli