Measurement of the sperm binding capacity of the mouse zona pellucida and its use in the estimation of anti-zona antibody titres.
Conditions have been defined under which the sperm binding capacity of the mouse zona pellucida can be measured. As a measure of the concentration of anti-zona antibodies the inhibition of sperm binding appears to be both repeatable and sensitive. Using this technique anti-zona antibody titres were monitored in 6 rats actively immunized with extracts of mouse ovarian tissue. These animals exhibited a rapid rise in anti-zona antibody titre following the induction of immunity, and an associated significant (P less than 0.05) decline in their fertility, both in relation to the proportions of animals exhibiting fertile matings and of matings resulting in conception. Three of the animals exhibited permanent sterility despite repeated exposure to fertile males.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D003165:Complement System Proteins / Q000276:immunology; D005260:Female; D005306:Fertilization; D005455:Fluorescent Antibody Technique; D008297:Male; D051379:Mice; D010063:Ovum / Q000276:immunology*; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology; D015044:Zona Pellucida / Q000276:immunology* / Q000502:physiology
D000906:Antibodies; D003165:Complement System Proteins