The strain A anti-p-azophenylarsonate major cross-reactive idiotypic family includes members with no reactivity toward p-azophenylarsonate.
The sera of immunized A/J mice contain low but detectable levels of immunoglobulin, bearing previously described cross-reactive idiotypic (CRI) determinants diagnostic of the strain A anti-p-azophenylarsonate (Ars) response. Such molecules from nonimmune sera cannot be adsorbed onto affinity columns coupled to a high density with Ars. After extensive immunization with Ars-coupled proteins, Ars-nonbinding CRI is found at the same low level, even though substantial Ars-binding CRI appears. On the other hand, immunization with a monoclonal rat anti-CRI, which was originally raised against Ars-binding CRI, elicits high concentrations of Ars-binding as well as Ars-nonbinding CRI immunoglobulin. Three hybridoma proteins produced from an A/J mouse immunized with the rat anti-CRI react with all tested anti-idiotypic sera from three species of animals, but show no reactivity toward Ars in several different assays. One hybridoma protein from the same fusion demonstrates Ars-binding capacity.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001391:Azo Compounds / Q000276:immunology*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D003429:Cross Reactions; D007106:Immune Sera / Q000494:pharmacology; D007130:Immunoglobulin Idiotypes; D007163:Immunosorbent Techniques; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D010132:p-Azobenzenearsonate / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001391:Azo Compounds; D007106:Immune Sera; D007130:Immunoglobulin Idiotypes; D010132:p-Azobenzenearsonate