Transfer of RP4::Mu to Salmonella typhimurium.
Restriction-proficient strains of Salmonella typhimurium are shown to be ineffective as recipients of normal RP4::Mucts62 due to the operation of two restriction systems (hsdSA and hsdLT) on the Mu moiety of this plasmid. Strains mutant in both these hsd loci are excellent recipients.
D001435:Bacteriophages / Q000235:genetics; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics; D009154:Mutation; D010957:Plasmids; D011815:R Factors; D012486:Salmonella typhimurium / Q000235:genetics*