Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28.
The purification and properties of a cytotoxic mouse monoclonal anti-HLA-A2 antibody are described. This antibody, BB7.2, can be used as an HLA-A2 typing reagent with little modification of current typing techniques. It also recognizes a low frequency variant of HLA-A28. BB7.2 provides an example of an antibody which requires bivalent attachment to a polymeric antigen, e.g., a cell, to produce a readily detectable complex. This is due to a high rate of dissociation of the complex formed between a single BB7.2 combining sites and HLA-A2. The consequences of this property were investigated and some complications for potential uses of such monoclonal antibodies are discussed.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000276:immunology; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology; D005591:Chemical Fractionation; D003602:Cytotoxicity, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D006680:HLA Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G / Q000302:isolation & purification; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010447:Peptide Hydrolases / Q000494:pharmacology; D011817:Rabbits
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D010447:Peptide Hydrolases