A sensitive and quantitative fluorescence assay for cell surface antigens.
A procedure is described for the assay of cell surface antigens based on quantitative fluorometry. Fluorescent immunospheres are coupled with sheep anti-mouse immunoglobulins or Protein A and used to detect specific antibody bound to target cells. The fluorescent sphere assay described here offers 16--128-fold greater sensitivity than complement mediated lysis or Protein A radioimmune assays and comparable sensitivity to rosetting assays. In addition, the assay is simple to perform, uses commercially available reagents and is completely objective in that a common laboratory fluorometer is used to obtain fluorescence measurements.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D005470:Fluorometry; D006801:Humans
D000954:Antigens, Surface