Cell structure and percent viability by a slide centrifuge technique.
It was found that a slide centrifuge (Cytospin) preparation of a cell suspension allowed a reliable assessment of not only cell structure but also the percentage of non-viable cells. The non-viable cells appeared as "smear" cells and paralleled in number the cells taking up trypan blue. Direct experiment showed the unstained viable cells in a trypan blue cell suspension remained intact in a Cytospin preparation while the cells taking up trypan blue were the "smear" cells. The non-viability of the "smear" cells was confirmed by their inability to survive in culture.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002498:Centrifugation / Q000379:methods; D003584:Cytological Techniques; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D051379:Mice; D014343:Trypan Blue
D014343:Trypan Blue