Nitrocellulose immunofixation following agarose electrophoresis in the study of immunoglobulin G subgroups in unconcentrated cerebrospinal fluid.
A method is presented which enables the study of cerebrospinal fluid proteins in minimal volumes of unconcentrated fluid. The method involves agarose electrophoresis followed by transfer of the proteins to a nitrocellulose membrane and then demonstration of their distribution using a double-antibody immunoperoxidase technique. The method is used here for the study of immunoglobulin G and its subgroups, and clearly shows that the oligoclonal pattern observed in multiple sclerosis is not restricted to any one subgroup.
D003101:Collodion; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid*; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid*
D007074:Immunoglobulin G; D003101:Collodion