Normal neutrophil function in human renal allograft recipients receiving Minnesota anti-lymphoblast globulin (MAG).
Equine anti-human lymphoblast globulin (MAG) used in combination with other immunosuppressive agents did not impair chemotaxis, phagocytosis, nitroblue tetrazolium (NBT) reduction or halogenation by peripheral blood neutrophils (PMN) from seven human recipients of cadaver renal allografts. MAG was given to the allograft recipients following transplantation, and the dosage was determined each day by quantitation of peripheral blood T and B lymphocytes. No deposition of immunoglobulin or complement on the recipient's PMN was detected by indirect immunofluorescence. Chemotaxis of PMN was determined in blind-well chambers, and phagocytosis of opsonized zymosan was quantitated by spectrophotometric measurement of solubilized formazan, and halogenation was measured by quantitating the incorporation of 125I into PMN that had phagocytosed opsonized zymosan. Chemotaxis, phagocytosis, NBT reduction, and halogenation by neutrophils from patients receiving MAG, prednisone and azathioprine were not impaired.
D000328:Adult; D000961:Antilymphocyte Serum / Q000494:pharmacology* / Q000627:therapeutic use; D001402:B-Lymphocytes / Q000276:immunology; D002633:Chemotaxis; D003165:Complement System Proteins / Q000032:analysis; D005260:Female; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D009504:Neutrophils / Q000276:immunology*; D009580:Nitroblue Tetrazolium; D010587:Phagocytosis; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology; D014184:Transplantation, Homologous
D000961:Antilymphocyte Serum; D011947:Receptors, Antigen, B-Cell; D009580:Nitroblue Tetrazolium; D003165:Complement System Proteins