3,3',5,5' - Tetramethylbenzidine as an Ames test negative chromogen for horse-radish peroxidase in enzyme-immunoassay.
The use of 3,3',5,5' - tetramethylbenzidine as non-mutagenic chromogen for the end point determination in enzyme-immunoassay (EIA) is described. In sandwich EIAs for HCG and HBsAg and in a competitive EIA for testosterone, the colour yield with TMB was superior to that obtained with o-phenylene diamine (OPD), which was by far the best chromogen for horse-radish peroxidase until now. This led to an improvement of sensitivity and precision of the assays and makes EIA even more competitive with other types of immunoassays.
D000818:Animals; D001560:Benzidines / Q000494:pharmacology*; D002417:Cattle; D006063:Chorionic Gonadotropin / Q000032:analysis; D002863:Chromogenic Compounds / Q000494:pharmacology*; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006735:Horseradish Peroxidase / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D009152:Mutagenicity Tests; D010544:Peroxidases / Q000378:metabolism*; D011817:Rabbits; D012995:Solubility; D013739:Testosterone / Q000032:analysis
D001560:Benzidines; D006063:Chorionic Gonadotropin; D002863:Chromogenic Compounds; D006514:Hepatitis B Surface Antigens; C021758:3,3',5,5'-tetramethylbenzidine; D013739:Testosterone; D006735:Horseradish Peroxidase; D010544:Peroxidases