Presumptive identification of streptococci with a new test system.
A test is described that could replace bacitracin susceptibility for presumptive identification of group A streptococci as well as 6.5% NaCl agar tolerance for presumptive identification of enterococcal streptococci. The L-pyrrolidonyl-beta-naphthylamide test, based on hydrolysis of pyrrolidonyl-beta-naphthylamide, was used in conjunction with the CAMP and bile-esculin tests to presumptively identify the streptococci. Among the beta-hemolytic streptococci; 98% of 50 group A, 98% of 46 group B, and 100% of 70 strains that were not group A, B, or D were correctly identified by the new presumptive test scheme. Among the non-beta-hemolytic streptococci; 96% of 74 group D enterococcal, 100% of 30 group D nonenterococcal, and 82% of 112 viridans strains were correctly identified by the new presumptive test scheme.
D001414:Bacitracin / Q000494:pharmacology; D001431:Bacteriological Techniques; D001646:Bile / Q000378:metabolism; D004929:Esculin / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D011760:Pyrrolidinones / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013291:Streptococcus / Q000145:classification* / Q000502:physiology; D013292:Streptococcus agalactiae / Q000145:classification*; D013297:Streptococcus pyogenes / Q000145:classification*
D011760:Pyrrolidinones; D001414:Bacitracin; D004929:Esculin; C034099:pyrrolidonyl-beta-naphthylamide; D012965:Sodium Chloride