[Affinity labeling of the ribosomes from Escherichia coli by 4-(N-2-chloroethyl-N-methyl-aminobenzyl-5'-phosphoamide of heptauridylic acid].
4-(N-2-chloroethyl-N-methyl)aminobenzyl-5'-phosphoamide of the heptauridylic acid was used to localize the structures organizing mRNA-binding site of ribosomes from E. coli. Both rRNA and proteins undergo modification in the ribosome 10-18 and 82-90% of the total ribosomal subunit modification, respectively. Using the method of two-dimensional electrophoresis in the acrylamide gel the proteins S5, S11, S13 and L2, L7/L12, L31 which are labelled by the analog of mRNA were identified. We assume these proteins to be located in the vicinity of mRNA-binding centre of ribosomes.
D000345:Affinity Labels / Q000494:pharmacology*; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D012333:RNA, Messenger / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D014500:Uracil Nucleotides / Q000494:pharmacology*; D014542:Uridine Monophosphate / Q000031:analogs & derivatives / Q000494:pharmacology*
D000345:Affinity Labels; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D014500:Uracil Nucleotides; C032110:4-(N-2-chloroethyl-N-methyl)aminobenzyl-5'-phosphoamide heptauridylate; D014542:Uridine Monophosphate
Affinnaia modifikatsiia ribosom Escherichia coli 4-[(N-2-khlorétil-N-metil)-amino]-benzil-5'-fosfamidom geptauridilovoĭ kisloty.