Immunocytochemical demonstration of the surface and cytoplasmic immunoglobulins of the rat bone marrow and blood cells.
The surface membrane associated immunoglobulins of rat blood and bone marrow lymphocytes, immunoglobulins within cytoplasm of plasma cells were demonstrated by indirect immunoperoxidase technique. About 38...42% of blood and 90% of bone marrow lymphocytes and positive-stained surface membranes. By ultrastructural characterization the different types of positive-stained lymphocytes of blood and bone marrow were recognized. The first type was characterized by high nucleo-cytoplasmic ratio and slight morphological differentiation. The other type of lymphocytes had more large cytoplasm and was rich in cellular organelles. Some blast cells, monocytes and plasma cells were also stained. The indirect immunoperoxidase technique was suggested to be quite suitable for identification of immunoglobulin-coating cells, mainly B-lymphocytes, their precursors and antibody-producing cells in light and electron microscopic levels.
D000818:Animals; D001853:Bone Marrow / Q000276:immunology*; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000032:analysis*; D008214:Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D051381:Rats
D007136:Immunoglobulins