The effect of limited proteolysis on rabbit muscle creatine kinase.
Creatine kinase from rabbit muscle is inactivated by limited proteolysis with proteinase K from Tritirachium album. Gel-filtration and cross-linking studies showed that the limited proteolysis did not affect the molecular weight of the enzyme under non-denaturing conditions, but did cause changes in the reactivity of the reactive thiol group on each subunit and in the ability of the enzyme to form a 'transition-state analogue' complex in the presence of magnesium acetate plus ADP plus creatinine plus NaNO3.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002850:Chromatography, Gel; D003402:Creatine Kinase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism; D019286:Endopeptidase K; D010450:Endopeptidases / Q000494:pharmacology*; D007461:Iodoacetates / Q000378:metabolism; D019807:Iodoacetic Acid; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D011485:Protein Binding; D011817:Rabbits
D007461:Iodoacetates; D000244:Adenosine Diphosphate; D003402:Creatine Kinase; D010450:Endopeptidases; D019286:Endopeptidase K; D003545:Cysteine; D019807:Iodoacetic Acid