Mechanism of ultraviolet-induced mutagenesis: the coding properties of ultraviolet-irradiated poly(dC) replicated by E. coli DNA polymerase I.
We have identified three lesions rather than cyclobutane dimers which alter the properties of UV-irradiated poly(dC) as a template for E.coli DNA polymerase I, and have characterised these lesions with respect to their coding properties, rates of formation and decay, and their sensitivity to uracil DNA glycosylase. Our results lead us to conclude that these lesions are (1) cytosine hydrates, which code for cytosine and to a lesser extent thymine, (2) uracil hydrates, which code for adenine and are not sensitive to uracil DNA glycosylase, and (3) uracils, which code for adenine and are removed by uracil DNA glycosylase.
D004256:DNA Polymerase I / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D009153:Mutagens; D011066:Poly C / Q000528:radiation effects*; D011131:Polyribonucleotides / Q000528:radiation effects*; D013698:Templates, Genetic; D014466:Ultraviolet Rays
D009153:Mutagens; D011131:Polyribonucleotides; C023826:poly(dC); D011066:Poly C; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase