Nucleic acid metabolism in yeast II. Metabolism of thymidylate during thymidylate excess death.
A discrete class of strains of Saccharomyces cerevisiae, able to utilize, highly efficiently, exogenous deoxythymidine-5'-monophosphate (dTMP),was found to be sensitive to concentrations greater than 10 Micro M dTMP in an otherwise complete growth medium. Excess dTMP is cytostatic and cytotoxic: 90% of exponentially growing cells lose colony forming ability within 1 h of exposure to excess dTMP is a growth medium. Uptake of dTMP, adenine, histidine, and leucine does occur during this thymidylate excess death (TED). dTMP is anabolized to higher phosphorylated synthesis is blocked under TED-conditions but not RNA and protein biosynthesis.
D001692:Biological Transport; D002470:Cell Survival / Q000187:drug effects; D004271:DNA, Fungal / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013938:Thymidine Monophosphate / Q000378:metabolism* / Q000633:toxicity; D013942:Thymine Nucleotides / Q000378:metabolism*
D004271:DNA, Fungal; D013942:Thymine Nucleotides; D013938:Thymidine Monophosphate