title: Evaluation of cell lines and immunofluorescence and plaque assay procedures for quantifying reoviruses in sewage.
abstract: Twelve continuous cell lines were tested to determine their sensitivity to reovirus types 1, 2, and 3 isolated from sewage. Madin-Darby bovine kidney (MDBK), rhesus monkey kidney (LLC-MK2), and human embryonic intestinal (intestinal 407) cells were most sensitive, respectively. In a similar study, MDBK cells were more sensitive than LLC-MK2 and Buffalo green monkey kidney (BGM) cells to sewage-isolated, protamine-precipitated reoviruses which had not been serotyped and had no previous cell contact. Sewage-isolated, protamine-precipitated reoviruses were also used in conjunction with MDBK cells in a comparative evaluation of immunofluorescent cell count and plaque assay procedures. The immunofluorescence assay is more sensitive and more rapid than the plaque assay. Reoviruses in excess of 10(4)/liter of raw sewage were detected by the immunofluorescent cell count assay.
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D002472:Cell Transformation, Viral; D004622:Embryo, Mammalian; D005455:Fluorescent Antibody Technique; D006801:Humans; D007422:Intestines; D007668:Kidney; D008253:Macaca mulatta; D012087:Reoviridae / Q000235:genetics / Q000302:isolation & purification*; D012722:Sewage / Q000032:analysis*
chemical_list: D012722:Sewage