title: Study of inulase from Debaryomyces phaffii Capriotti.
abstract: Debaryomyces phaffii possesses an inulinase activity which can be induced by growth on beta-fructosidase and particularly on inulin. The enzyme is located in the cell wall but is easily excreted into the culture medium. Maximum activity on inulin is observed at pH 4 and 50 degrees C. The Km on inulin is 1.2 X 10(-2) M. The enzyme breaks down inulin by splitting off terminal fructosyl units and it is active on sucrose and raffinose.
mesh_terms: D001203:Ascomycota / Q000201:enzymology*; D004790:Enzyme Induction; D005947:Glucose; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007328:Insulin; D007444:Inulin / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D004718:Saccharomycetales / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D007328:Insulin; D007444:Inulin; D006026:Glycoside Hydrolases; C023086:inulinase; D005947:Glucose