Determination of terminal deoxynucleotidyl transferase on bone marrow smears by immunoperoxidase.
Terminal deoxynucleotidyl transferase (TdT) is a marker of lymphoblastic disorders. Previously, the technique for determining TdT on smears was by indirect immunofluorescence. An immunoperoxidase procedure for detecting TdT on smears is reported. Results are in a comparable range with the indirect immunofluorescent technique. As discussed in this paper, TdT by immunoperoxidase may offer certain advantages over the indirect immunofluorescent procedure.
D001853:Bone Marrow / Q000201:enzymology*; D004253:DNA Nucleotidylexotransferase / Q000032:analysis*; D004254:DNA Nucleotidyltransferases / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007945:Leukemia, Lymphoid / Q000175:diagnosis / Q000201:enzymology*
D004254:DNA Nucleotidyltransferases; D004253:DNA Nucleotidylexotransferase