Improved bioassay for insulin by continuous glucose monitoring.
The USP bioassay for insulin currently used by industry and government requires a sc injection of insulin to rabbits and multiple assays of blood glucose. One hundred and forty-four rabbits are usually assayed, each assay taking at least 2 weeks. A bioassay for insulin is described. The new assay is based on a continuous monitoring of blood glucose and repeated iv injection of insulin to dogs. The test is completed in 1 day and is carried out on one dog. The interassay variability of seven repeated tests of an off the shelf soluble insulin was +/- 8%.
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D001786:Blood Glucose / Q000378:metabolism*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D005260:Female; D007328:Insulin / Q000032:analysis* / Q000494:pharmacology
D001786:Blood Glucose; D007328:Insulin