Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems.
As biotin-avidin systems continue to be developed for applications involving single cells, cell suspensions, and especially tissue sections, the need arises for a method of blocking endogenous avidin-binding activity. One such method is described and its proposed mechanism is discussed. Utilizing this method, endogenous avidin-binding activity was detected and suppressed in selected human and murine tissues, thus facilitating the interpretation of specific immunohistochemical staining utilizing hybridoma monoclonal antibodies in a biotin-avidin-horseradish peroxidase detection system.
D000818:Animals; D000911:Antibodies, Monoclonal; D001360:Avidin / Q000378:metabolism*; D001710:Biotin / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism; D051379:Mice; D010047:Ovalbumin / Q000031:analogs & derivatives*; D011485:Protein Binding; D012867:Skin / Q000378:metabolism; D013154:Spleen / Q000378:metabolism
D000911:Antibodies, Monoclonal; D001360:Avidin; D001710:Biotin; D010047:Ovalbumin