title: Agents that cause a high frequency of genetic change from [psi+] to [psi-] in Saccharomyces cerevisiae.
abstract: The [psi] factor of yeast is cytoplasmically inherited. Singh, Helms and Sherman (1979) reported that high concentrations of KCl and of ethylene glycol induce the genetic change from [psi+] to [psi-]. In this study, the following agents have been shown to induce the same genetic change: guanidine hydrochloride at 1 mM, dimethyl sulfoxide at 2.5% v/v and ethanol or methanol at 10% v/v. It is likely that a number of other agents also cause the change, namely 2 M glycerol, M succinate, M glutamate and M MgCl2. Most of these agents induce the change at very high frequencies; with some, the frequency is 100%. Although the observed phenotypic change can also occur as a result of chromosomal gene mutation, no changes of this type were identified. Some of the agents also cause mutation from [rho+] to [rho-] and from killer to sensitive.
mesh_terms: D004271:DNA, Fungal / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D006146:Guanidines / Q000494:pharmacology; D000432:Methanol / Q000494:pharmacology; D009153:Mutagens; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013489:Suppression, Genetic
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D006146:Guanidines; D009153:Mutagens; D000431:Ethanol; D000432:Methanol; D004121:Dimethyl Sulfoxide