Characterization of the human cellular immune response to purified group A streptococcal blastogen A1.
The human mononuclear cell response to purified extracellular streptococcal protein, blastogen A, was compared to the response of these cells to PHA and tetanus antigen. Blastogen A induced peak uptake of thymidine during day 6 of tissue culture whereas PHA induced peak uptake during day 5 and tetanus during day 8. Like PHA, blastogen A transformed human umbilical cord lymphocytes and those of nonimmune animals. Also like PHA, blastogen A transformed primarily T lymphocytes. However, unlike PHA, the ability of T lymphocytes to respond to blastogen A was almost completely dependent on the presence of viable non-T lymphocytes. Monocytes were not as effective in facilitating the response to blastogen A as they were for PHA. Thus, blastogen A behaves most like a polyclonal T lymphocyte mitogen, although the degree of dependence of the transformation response on the presence of non-T lymphocytes is much greater than that of PHA.
D000818:Animals; D000911:Antibodies, Monoclonal; D055598:Chemical Phenomena; D002621:Chemistry; D002682:Chinchilla; D006801:Humans; D007111:Immunity, Cellular; D008213:Lymphocyte Activation / Q000187:drug effects; D008222:Lymphokines / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D012756:Sheep; D013297:Streptococcus pyogenes / Q000276:immunology; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014470:Umbilical Cord / Q000166:cytology
D000911:Antibodies, Monoclonal; D008222:Lymphokines; D010835:Phytohemagglutinins; C027779:blastogenic factor; D013936:Thymidine