In vitro induction of monoclonal antibody-defined T-cell markers in lymphocytes from immunodeficient children by synthetic serum thymic factor (FTS).
Lymphocytes from five children suffering from ataxia telangectasia or various unclassified immune deficiencies were tested in vitro for their sensitivity to synthetic serum thymic factor (FTS). The percentages of cells bearing T cell markers were elevated after incubation with FTS at graded concentrations (0.25, 2.5 and 25 ng/ml), by microlymphocytotoxicity or indirect immunofluorescence, using monoclonal anti-Lyt1 antibodies. In four cases, more than 30% of the non-T non-B cells acquired the Lyt1 T cell marker. These four children had low levels of circulating FTS. In the fifth child, who had a normal serum FTS level, and in two age-matched controls, there was no significant increase in the percentage of cells bearing the T marker.
D000293:Adolescent; D000911:Antibodies, Monoclonal / Q000276:immunology; D001260:Ataxia Telangiectasia / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000276:immunology*; D007958:Leukocyte Count; D008297:Male; D013601:T-Lymphocytes / Q000276:immunology*; D013935:Thymic Factor, Circulating / Q000276:immunology*; D013951:Thymus Hormones / Q000276:immunology*
D000911:Antibodies, Monoclonal; D013951:Thymus Hormones; D013935:Thymic Factor, Circulating