title: Genetic stability of differentiated functions in Streptomyces hygroscopicus in relation to conditions of continuous culture.
abstract: The genetic stability of the capacity of an improved strain of Streptomyces hygroscopicus to produce the macrolide antibiotic turimycin was investigated during long-term continuous culture. Dilution rate, growth-limiting substrate and culture temperature were varied. Certain culture conditions resulted in the stable propagation of the inoculated turimycin-producing population. Other conditions led to segregation of the initial population. Turimycin non-producing phenotypes appeared, and in each case the simultaneous loss of ability to form aerial mycelium was observed. The non-differentiating clones were found to be stable, without any reversion to the parental phenotype, indicating that a loss of genetic information probably took place.
mesh_terms: D000643:Ammonium Chloride / Q000494:pharmacology; D001431:Bacteriological Techniques; D005947:Glucose / Q000494:pharmacology; D007933:Leucomycins / Q000096:biosynthesis*; D010641:Phenotype; D010957:Plasmids; D013302:Streptomyces / Q000235:genetics*; D013696:Temperature; D013997:Time Factors
chemical_list: D007933:Leucomycins; D000643:Ammonium Chloride; C011138:turimycin; D005947:Glucose