An immunoradiometric quantitative assay of Escherichia coli recA protein.
A two-site immunoradiometric assay of Escherichia coli recA protein is described ; its sensitivity allows the detection of 0.1 ng of recA protein ; it yields a linear response for amounts of recA protein in the 0.1-7 ng range. It can be directly applied to extracts of Escherichia coli obtained by sonication. Salmonella typhimurium extracts contain some cross-reacting material which share common antigenic determinants with Escherichia coli recA protein but differ from it.
D000937:Antigen-Antibody Reactions; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D003429:Cross Reactions; D004926:Escherichia coli / Q000032:analysis*; D007074:Immunoglobulin G / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D011938:Rec A Recombinases; D012486:Salmonella typhimurium / Q000276:immunology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D011938:Rec A Recombinases