Purification of a lethal toxin produced by Shigella dysenteriae.
A lethal toxin was purified from the culture supernatant of Shigella dysenteriae 1. The purification procedure utilized ammonium sulfate fractionation, column chromatography on DEAE-cellulose, CM-cellulose, hydroxylapatite and gel filtration on Sephadex G-200. About a 4760-fold purification was achieved, with a yield of 2.7%. The purified lethal toxin appeared homogeneous on polyacrylamide gel disc electrophoresis. The LD50 of the purified lethal toxin in mice following i.p. injection was about 7.5 micrograms/kg. Injection i.p. of 2 LD50 of the purified lethal toxin into mice caused paralysis of the limbs followed by death after 2-4 days.
D000818:Animals; D001427:Bacterial Toxins / Q000302:isolation & purification* / Q000633:toxicity; D002845:Chromatography; D004405:Dysentery, Bacillary / Q000209:etiology; D007928:Lethal Dose 50; D051379:Mice; D010243:Paralysis / Q000139:chemically induced; D022603:Shiga Toxins; D012762:Shigella dysenteriae
D001427:Bacterial Toxins; D022603:Shiga Toxins