title: Evidence that translocation of the glucose transport activity is the major mechanism of insulin action on glucose transport in fat cells.
abstract: The glucose transport activity associated with the plasma membrane-rich and Golgi-rich fractions of fat cells was determined after they were reconstituted into egg lecithin liposomes. When the two subcellular fractions were isolated under conditions that would minimize their cross-contamination, the transport activity in the plasma membrane-rich fraction was found to be increased 6.3- to 8.6-fold by insulin, which was added to cells before homogenization, and that the activity in the Golgi-rich fraction was reduced approximately to one-half. In this study, the glucose transport activity in the plasma membrane-rich fraction (either in the basal or plus insulin state) was solubilized, reconstituted, and assayed with an overall efficiency of 25-35%. Four agents known to have insulin-like effects on the glucose transport activity in intact fat cells (hydrogen peroxide, sodium vanadate, trypsin, and p-chloromercuriphenyl sulfonate) not only increased the transport activity in the plasma membrane-rich fraction, but also decreased the activity in the Golgi-rich fraction. The effect of hydrogen peroxide, unlike that of insulin, was not abolished when the insulin receptor was modified proteolytically. Upon administration of insulin to fat cells, and subsequent elimination of the hormone, the glucose transport activities associated with the plasma membrane-rich and Golgi-rich fractions were affected almost concomitantly towards opposite directions. It is proposed as a working hypothesis that translocation of the glucose transport system to the plasma membrane from the Golgi-rich fraction is the major, if not the sole, mechanism by which insulin stimulates glucose transport in fat cells.
mesh_terms: D019325:3-O-Methylglucose; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D003847:Deoxyglucose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D008757:Methylglucosides / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007328:Insulin; D008757:Methylglucosides; D019325:3-O-Methylglucose; D003847:Deoxyglucose; D005947:Glucose