In vitro cultivation of Treponema pallidum: independent confirmation.
In vitro cultivation of the virulent Nichols strain of Treponema pallidum was achieved in a tissue culture system as described by A. H. Fieldsteel, D. L. Cox, and R. A. Moeckli (Infect. Immun. 32:908-915, 1981). In 7 of 8 experiments, 8.9- to 26.2-fold increases in the number of T. pallidum were observed over a 12- to 12-day period of incubation.
D001431:Bacteriological Techniques; D001769:Blood; D002460:Cell Line; D003470:Culture Media; D004229:Dithiothreitol / Q000494:pharmacology; D007700:Kinetics; D010100:Oxygen / Q000494:pharmacology; D014210:Treponema pallidum / Q000254:growth & development*
D003470:Culture Media; D010100:Oxygen; D004229:Dithiothreitol