title: Radioimmunoassay for yeast killer toxin from Saccharomyces cerevisiae.
abstract: A radioimmunoassay was developed for the K1 killer toxin from strain T158C/S14a of Saccharomyces cerevisiae. 125I-labeled toxin was made to a specific activity of 100 microCi/mg of protein (1 microCi = 37 kBq). Antibody to purified toxin was prepared in rabbits using toxin cross-linked to itself. These antibodies, partially purified by 50% ammonium sulfate precipitation and Sepharose CL-6B column chromatography, produced one precipitation band with killer toxin and bound 125I-labeled toxin in a radioimmunoassay. The antibody preparation also bound with the toxins from another K1 killer, A364A, and three chromosomal superkiller mutants derived from it.
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D055646:Killer Factors, Yeast; D009183:Mycotoxins / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000276:immunology; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D055646:Killer Factors, Yeast; D009183:Mycotoxins; D029701:Saccharomyces cerevisiae Proteins