Immunocytological detection of AAF-DNA adducts in HeLa cell nuclei.
Acetylaminofluorene-DNA adducts (AAF-DNA) were detected in the nuclei of HeLa cells exposed to N-acetoxy-2-acetylaminofluorene (N-Ac-AAF), using an immunocytological technique and specific antibodies directed against AAF modified DNA. The proportion of cells exhibiting specific nuclear immunoreactivity was dose-dependent. The time course of disappearance of adduct specific nuclear immunoreactivity was compared with removal of N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-C8-AAF) and other adducts.
D015073:2-Acetylaminofluorene / Q000378:metabolism*; D000974:Antibodies, Antinuclear / Q000032:analysis*; D000918:Antibody Specificity; D002467:Cell Nucleus / Q000276:immunology*; D004247:DNA / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells / Q000276:immunology / Q000648:ultrastructure; D006801:Humans; D013997:Time Factors
D000974:Antibodies, Antinuclear; D004247:DNA; D015073:2-Acetylaminofluorene