title: [Correlation between the biological activity of 30S subunits of Escherichia coli ribosomes and their conformational changes revealed by mixing optical spectroscopy].
abstract: The analysis of light spectra scattered from solution of 30S subunits were performed by the method of regularization of inverse spectral problem. The subunits observed at ionic conditions which preserved their biological activity (200 mM NH4Cl at 1 mM MgCl2) revealed a monodisperse pattern of scattering with diffusion constant D = (1,83 +/- 0.10) . 10(-7) cm2/sec. The polydispersity and compactization of 30S subunits was observed in inactivating ionic conditions (30 mM NH4Cl at 1 mM MgCl2). The number of compactized particles correlates with the irreversible loss of biological activity, the capability of 30S subunits to bind specific tRNA.
mesh_terms: D004926:Escherichia coli / Q000378:metabolism* / Q000648:ultrastructure; D007700:Kinetics; D012343:RNA, Transfer / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism* / Q000648:ultrastructure; D013057:Spectrum Analysis
chemical_list: D012343:RNA, Transfer
vernacular_title: Korreliatsiia mezhdu konformatsionnym izmenenniem, nabliudaemym metodom spektroskopii opticheskogo smesheniia, i biologicheskoĭ aktivnost'iu 30S subchastits ribosom Escherichia coli.