Two-site immunoenzymometric assay for thyrotropin in dried blood samples on filter paper.
We describe a sensitive, simple, and rapid two-site immunoenzymometric assay for thyrotropin in dried blood samples on filter paper, for use in screening for neonatal primary hypothyroidism. In this method, two dried-blood spots of 3 mm diameter (equivalent to about 5.4 microliter of blood) are incubated overnight with anti-thyrotropin-beta-D-galactosidase complex in an anti-thyrotropin coated tube. Then the enzyme activity in the washed tube is determined fluorophotometrically. The range of thyrotropin measurable is 10 to 160 milli-int. units/L blood. Values for thyrotropin in dried blood samples determined by this method and those determined by radioimmunoassay correlated highly (r = 0.96).
D001800:Blood Specimen Collection / Q000379:methods; D003409:Congenital Hypothyroidism; D006801:Humans; D007037:Hypothyroidism / Q000175:diagnosis; D007124:Immunoenzyme Techniques; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000175:diagnosis; D008403:Mass Screening; D010209:Paper; D013972:Thyrotropin / Q000097:blood*
D013972:Thyrotropin