Insulin-potentiating action of human growth hormone. Synthesis and activity of N-terminal fragments.
Four peptides from the N-terminal region of human growth hormone have been synthesized by the solid-phase method: hGH(6-13), hGH(7-13), hGH(8-13) and hGH(9-13). Although these peptides contain the sensitive -Asp-Asn-sequence, apparently homogeneous products were obtained by synthesis on polystyrene resin, cleavage by hydrogen fluoride and purification by ion-exchange chromatography. The insulin-potentiating activity of these peptides is reported. The data indicates that extension of hGH(9-13) at its N-terminus is required for in vitro activity.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004357:Drug Synergism; D005947:Glucose / Q000378:metabolism; D013006:Growth Hormone / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D009132:Muscles / Q000378:metabolism; D010446:Peptide Fragments / Q000138:chemical synthesis; D051381:Rats; D011919:Rats, Inbred Strains
D000596:Amino Acids; D007328:Insulin; D010446:Peptide Fragments; D013006:Growth Hormone; D005947:Glucose