Lymphocyte sensitization detected by the macrophage electrophoretic mobility assay in patients with renal cell carcinoma: Theophylline increases the sensitivity of the assay.
An antigen-induced release of a macrophage slowing factor (MSF) by peripheral blood lymphocytes was used to evaluate lymphocyte sensitization to various antigens in 30 patients with renal cell carcinoma (RCC) and in 14 normal individuals. Twenty-three of 30 (77%) patients with RCC, but no healthy controls were found to be sensitized to a soluble antigen prepared from an allogeneic kidney tumor by 3 M potassium chloride extraction. Peripheral blood lymphocytes from some patients with RCC displayed sensitization to protein isolated from fetal kidney (6 of 24; 25%), control "normal" kidney (6 of 30; 20%) and urinary bladder carcinoma (3 of 21; 14%) tissues. It has been suggested that cyclic adenosine 3' ,5'-monophosphate (cyclic AMP) could play a role in the mechanism of the MSF action (24). In agreement with this idea, the presence of 10-4 M theophylline enhanced the macrophage electrophoretic mobility (MEM) reduction caused by MSF. Furthermore, the DNA synthesis in lymphocytes (monitored by measuring the uptake of tritiated thymidine) on contact with phytohemagglutinin (PHA) was depressed in 9 of 21 (43%) patients with RCC as compared with healthy controls.
D000230:Adenocarcinoma / Q000276:immunology*; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000276:immunology; D005260:Female; D006801:Humans; D007114:Immunization; D007122:Immunoelectrophoresis; D008214:Lymphocytes / Q000276:immunology*; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis; D008264:Macrophages / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D013806:Theophylline / Q000494:pharmacology*; D001749:Urinary Bladder Neoplasms / Q000276:immunology
D000951:Antigens, Neoplasm; D008263:Macrophage Migration-Inhibitory Factors; D013806:Theophylline