Microcalorimetric studies of Klebsiella aerogenes grown in chemostat culture. 2 C-limited and C-sufficient cultures.
The enthalpy changes for the production of 1 g of cells during a doubling period, delta Hg, for cells of K. aerogenes growing aerobically in carbon-limited or carbon-sufficient media in continuous culture have been measured. Delta Hg varies with the dilution rate, but at a fixed dilution rate delta Hg is the same for growth in glucose, glycerol, pyruvate or acetate as the sole carbon and energy source; the molar growth yields are markedly different. Delta Hg values of cells growing in glucose-sufficient media (i.e. N- or Mg-limitation) are much higher than in glucose-limited media. This suggests the presence of a regulating system for the degradation of the energy source. Anaerobic growth in glucose-limited media results in a reduction of the molar growth yield and an increase in the heat output. From a consideration of the yield and delta Hg values it is concluded that the amount of energy lost (as heat) per mol of glucose during glycolysis is the same under aerobic and anaerobic conditions.
D000332:Aerobiosis; D000693:Anaerobiosis; D002151:Calorimetry; D050260:Carbohydrate Metabolism; D002264:Carboxylic Acids / Q000378:metabolism*; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000254:growth & development / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D013816:Thermodynamics
D002264:Carboxylic Acids; D005947:Glucose; D009584:Nitrogen; D005990:Glycerol