Use of permeabilized yeast cells as a system of enzyme immobilization. Its use for the preparation of mannose 6-phosphate.
It was shown that hexokinase contained inside permeabilized yeast cells can be used successfully in the phosphorylation of mannose for the production of mannose 6-phosphate. We propose that enzymes entrapped within the semipermeable cell membrane be considered as an extension of the enzyme immobilization concept, since it shares many of its advantages and several of its properties with some unique characteristics.
D002462:Cell Membrane / Q000201:enzymology; D002463:Cell Membrane Permeability / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism*; D006600:Hexosephosphates / Q000096:biosynthesis*; D008358:Mannose / Q000378:metabolism*; D008359:Mannose-6-Phosphate Isomerase / Q000235:genetics; D008360:Mannosephosphates / Q000096:biosynthesis*; D009154:Mutation; D010766:Phosphorylation; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics
D004800:Enzymes, Immobilized; D006600:Hexosephosphates; D008360:Mannosephosphates; C027693:mannose-6-phosphate; D006593:Hexokinase; D008359:Mannose-6-Phosphate Isomerase; D008358:Mannose; D004121:Dimethyl Sulfoxide