Spontaneous production of lymphocyte migration inhibitory factor (sLyMIF) by T and B lymphocytes from peripheral rabbit blood.
Nonstimulated 24 h cultures of lymphocytes from rabbit peripheral blood, as well as isolated rabbit T and B lymphocytes release into the culture medium a factor inhibiting migration of T and B lymphocytes (LyMIF). Synthesis of the factor can be blocked by puromycin. The stimulatory factor for B lymphocyte migration (LyMSF) can also be produced in T lymphocyte cultures. The synthesis of both factors occurs in suspensions containing monocytes, as well as in those lacking monocytes.
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D002464:Cell Migration Inhibition; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D007166:Immunosuppressive Agents; D066298:In Vitro Techniques; D007961:Leukocyte Migration-Inhibitory Factors / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D008222:Lymphokines / Q000096:biosynthesis*; D008297:Male; D011691:Puromycin / Q000494:pharmacology; D011817:Rabbits; D013601:T-Lymphocytes / Q000378:metabolism*
D003470:Culture Media; D007166:Immunosuppressive Agents; D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D011691:Puromycin