An efficient technique for the isolation of yeast spores and the preparation of spheroplast lysates active in protein synthesis.
D001173:Arthrobacter / Q000201:enzymology; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D005966:Glucuronidase; D006026:Glycoside Hydrolases; D007700:Kinetics; D008722:Methods; D009097:Multienzyme Complexes; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D013104:Spheroplasts / Q000378:metabolism*; D013170:Spores / Q000302:isolation & purification*; D013429:Sulfatases; D014027:Tobacco Mosaic Virus
D009097:Multienzyme Complexes; D012333:RNA, Messenger; D012367:RNA, Viral; C023059:glusulase; D013429:Sulfatases; D006026:Glycoside Hydrolases; D005966:Glucuronidase; D004693:Glucan Endo-1,3-beta-D-Glucosidase