A minimal mechanism for abortive initiation of transcription of T7 DNA.
A steady state kinetic analysis of product inhibition in abortive initiation of transcription at promoters A1 and A3 of T7 DNA was carried out. The obtained kinetic data are in agreement with a mechanism of ordered product release with pppApU being released first from the central complex. A minimal general mechanism for abortive initiation satisfying the kinetic results is given. The stimulation of transcription of T7 DNA by pppApU at low substrate concentrations was investigated.
D004279:DNA, Viral / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D007700:Kinetics; D008433:Mathematics; D013604:T-Phages / Q000235:genetics*; D014158:Transcription, Genetic
D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases