A high frequency idiotypic marker of anti-DNA autoantibodies in MRL-Ipr/Ipr mice.
Anti-idiotypic antibodies were used to analyze relatedness of anti-DNA autoantibodies of MRL mice. The antiidiotypic antibodies, produced by immunization of rabbits with MRL-Ipr-derived hybridoma anti-DNA antibodies, were defined by their ability to inhibit specifically the binding of hybridoma anti-DNA antibody to DNA. One monoclonal anti-DNA antibody, H130, cross-reacted with two thirds of other hybridoma anti-DNA antibodies. The H130/anti-H130 system detected high amounts (up to 5 mg/ml) of immunoreactivity in sera of MRL-Ipr mice, whereas levels in MRL-+ sera were relatively low. The H130 family increased 10-fold in MRL-Ipr mice over the course of 5 mo, but its levels did not correlate chronologically with those of anti-DNA antibodies, cardiolipin-binding antibodies, or total serum immunoglobulins. H130 cross-reactivity was found, by quantitative immunoadsorption, in 40 to 60% of serum anti-DNA antibodies; however, a large excess of H130-related serum antibodies did not bind to DNA. The H130 family and anti-DNA antibodies therefore seem to constitute overlapping populations.
D000375:Aging; D000818:Animals; D001323:Autoantibodies / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002308:Cardiolipins / Q000378:metabolism; D004247:DNA / Q000276:immunology*; D004277:DNA, Single-Stranded / Q000276:immunology; D005787:Gene Frequency; D007074:Immunoglobulin G / Q000378:metabolism; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D007075:Immunoglobulin M; D007163:Immunosorbent Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D011817:Rabbits
D001323:Autoantibodies; D002308:Cardiolipins; D004277:DNA, Single-Stranded; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M; D004247:DNA