IgA-producing hybridomas are readily derived from gut-associated lymphoid tissue.
Eight hybridomas secreting IgA monoclonal antibodies were obtained by using gut-associated lymphoid tissue as a primed plasmablast source. IgA secretors are obtained at higher frequencies by this technique than by conventional splenic fusions. This technique provides useful tools for the study of mucosal protection, and it demonstrates that exaggerated potential of gut-derived B cells, compared to splenic B cells, for IgA expression.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D006825:Hybridomas / Q000276:immunology*; D007070:Immunoglobulin A / Q000096:biosynthesis*; D007158:Immunologic Techniques; D007422:Intestines / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008198:Lymph Nodes / Q000166:cytology; D008221:Lymphoid Tissue / Q000276:immunology*; D008297:Male; D008643:Mesentery / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000166:cytology; D051381:Rats; D011916:Rats, Inbred F344; D013154:Spleen / Q000166:cytology / Q000276:immunology
D007070:Immunoglobulin A; D008070:Lipopolysaccharides