Detection of sperm surface IgG by modified haemadsorption procedures.
New haemadsorption procedures for the detection of sperm bound IgG are described. Antibody-coated sperm are fixed with formaldehyde and attached by poly-L-lysine to the bases of wells on plastic plates where they can be left for several days if necessary. Sensitised indicator red cell (for direct haemadsorption) or anti-IgG sera followed by indicator red cells (for indirect haemadsorption) are then added to these wells and the presence of antibody indicated by red cell attachment to sperm. The procedure uses simple reagents and the sperm/red cell rosettes may be fixed and stained to be kept as records.
D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D006382:Hemadsorption; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007158:Immunologic Techniques; D007248:Infertility, Male / Q000276:immunology; D008297:Male; D011817:Rabbits; D013094:Spermatozoa / Q000276:immunology*
D007074:Immunoglobulin G