title: OKT 6-positive cells: their demonstration in human thymus, and the effect of fixation on the immunoperoxidase reaction.
abstract: Human thymocytes at an intermediate stage of differentiation were demonstrated in situ in tissue sections of thymus using the mouse monoclonal antibody OKT 6 THY (Ortho Immunobiology Ltd, Raritan, NJ) and the immunoperoxidase technique. These cells were found to reside mainly within the thymic cortex. The effects of on-slide fixation on the ease of demonstration of the antigen involved were assessed. The choice of fixative was found to have a marked effect, acetone and paraformaldehyde producing the most satisfactory results.
mesh_terms: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D002454:Cell Differentiation; D005069:Evaluation Studies as Topic; D005404:Fixatives; D006652:Histological Techniques; D006801:Humans; D007124:Immunoenzyme Techniques; D007231:Infant, Newborn; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D005404:Fixatives