Fibronectin from human germ-cell tumors resembles amniotic fluid fibronectin.
Three human germ-cell tumors established as xenografts in immunosuppressed mice were shown to produce fibronectin. Immunoperoxidase tissue staining with a specific antiserum revealed human fibronectin in some tumor cells. The cyst fluid which accumulated in the tumors contained human fibronectin as shown by a species-specific radioimmunoassay. Human fibronectin was isolated from two of the tumor fluids and compared to previously characterized fibronectins. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis and gel electrofocusing showed that the germ-cell fibronectins resembled amniotic fluid fibronectin with regard to subunit molecular weight and isoelectric point. The distinct features of the amniotic fluid and germ-cell tumor fibronectin suggest that such fibronectins may provide new oncodevelopmental markers.
D000653:Amniotic Fluid / Q000378:metabolism*; D000818:Animals; D004407:Dysgerminoma / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007525:Isoelectric Focusing; D008649:Mesonephroma / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000032:analysis; D010051:Ovarian Neoplasms / Q000378:metabolism*; D011863:Radioimmunoassay; D013724:Teratoma / Q000378:metabolism*
D005353:Fibronectins