title: Further investigations of mutagenic cholesterol preparations.
abstract: The previously demonstrated mutagenicity of naturally air-aged (autoxidized) USP cholesterol in test strains of Salmonella typhimurium has been confirmed. In contrast, autoxidized brassicasterol, 7-dehydrocholesterol, ergosterol, lanosterol, sitosterol, and stigmasterol were non-mutagenic in the same assay, and 38 individual cholesterol derivatives, including many cholesterol autoxidation products, were also non-mutagenic. The mutagenic species from mutagenic cholesterol preparations were shown to be neutral steroids that are very much more polar than the cholesterol autoxidation products so far identified. High-performance liquid chromatography resolved mutagenic cholesterol preparations into several mutagenic and non-mutagenic fractions. The chemical nature of the mutagens is suggested as involving oxidation of the sterol B-ring and of the side-chain.
mesh_terms: D002784:Cholesterol / Q000032:analysis / Q000633:toxicity*; D002851:Chromatography, High Pressure Liquid; D009153:Mutagens / Q000302:isolation & purification* / Q000633:toxicity; D010084:Oxidation-Reduction; D012486:Salmonella typhimurium / Q000187:drug effects
chemical_list: D009153:Mutagens; D002784:Cholesterol