[Possibility of the morphometry of the neuronal body and nucleus in cryostat sections of nerve tissue].
A method of fixation by the Carnoy liquid and of gallocyanine staining is proposed in order to obtain clear-cut boundaries of the neuron body, nucleus and nucleolus in cryostat sections of non-fixed frozen nerve tissue, for subsequent morphometry of the body and nucleus of nerve cells. In the resulting sections, the clearness of boundaries of the body and nucleus in a neuron is sufficient for measuring its diameter with the coefficient of variation not exceeding 2.5%.
D000818:Animals; D002466:Cell Nucleolus / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure*; D003584:Cytological Techniques; D005615:Freezing; D008253:Macaca mulatta; D009474:Neurons / Q000166:cytology*
O vozmozhnosti morfometrii tela i iadra neĭrona v kriostatnykh srezakh nervnoĭ tkani.