title: Different optimal PHA concentrations for stimulation of Chinese hamster lymphocytes in cultures supplemented with foetal calf serum or horse serum.
abstract: Chinese hamster lymphocyte cultures were supplemented with foetal calf serum (FCS) or hose serum (HS). Addition of 2-mercaptoethanol (2-ME) to cultures supplemented with these sera resulted in a marked increase lymphocyte proliferation. The PHA concentrations necessary for optimal lymphocyte stimulation were much lower in cultures supplemented with HS than with FCS. Phytohaemagglutinin (PHA) concentrations optimal for stimulation of a given number of lymphocytes in cultures supplemented with FCS often gave inhibition of similar cultures supplemented with HS.
mesh_terms: D000818:Animals; D001769:Blood; D002417:Cattle; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005312:Fetal Blood; D006736:Horses; D007158:Immunologic Techniques; D008213:Lymphocyte Activation; D008623:Mercaptoethanol / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology*; D011247:Pregnancy
chemical_list: D003470:Culture Media; D010835:Phytohemagglutinins; D008623:Mercaptoethanol