The role of polymorphonuclear leukocyte (PMNL) granule factors in the control of T cell dependent response in mice.
Factors isolated from human PMNL lysosomes granules enhance the immune response of BALB/c mice immunized with SRBC, augment allogeneic DTH reaction and accelerate skin graft rejection. The immunostimulatory effects does not depend only on proteolytic enzymes. Removal of proteolytic enzymes using DFP as irreversible inactivator did not alter the immunostimulatory effect of PMNL lysosome factors on PFC formation in mice. The PMNL factors have marked potentiating effect on the uptake and retention of labeled CRBC in mouse lymphoid system. This effect suggests an alternative pathway which can provide chronic immunological stimulus and play a significant role in augmentation of immune response. Data presented in this paper suggest that factors responsible for augmentation of the immune response are localized mostly in secondary PMNL granules.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D002645:Chickens; D004912:Erythrocytes / Q000276:immunology; D006084:Graft Rejection; D006462:Hemolytic Plaque Technique; D006968:Hypersensitivity, Delayed / Q000175:diagnosis; D008247:Lysosomes / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000276:immunology*; D010447:Peptide Hydrolases / Q000494:pharmacology; D011506:Proteins / Q000494:pharmacology*; D012397:Rosette Formation; D012756:Sheep; D016038:Skin Transplantation; D013601:T-Lymphocytes / Q000276:immunology*
D011506:Proteins; C018589:lysosomal proteins; D010447:Peptide Hydrolases