title: The nucleotide sequence of the HIS4 region of yeast.
abstract: We have determined the nucleotide sequence of the yeast HIS4 gene and its 5' and 3' flanking sequences. The protein chain has a calculated Mr-value of 87 935. Th 5' end of the HIS4 transcript maps at a position 63 bp upstream from the site of initiation of protein synthesis. The 3' end of the HIS4 transcript maps at a position approx. 118 bp after the UAG termination codon. There is no evidence for intervening sequences within the transcription unit. Functional sub-regions within the HIS4 coding frame have been identified by determining the sequence changes for various his4 mutations.
mesh_terms: D001483:Base Sequence; D003062:Codon; D004271:DNA, Fungal / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D005809:Genes, Regulator; D006639:Histidine / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D003062:Codon; D004271:DNA, Fungal; D012333:RNA, Messenger; D006639:Histidine