Prostacyclin metabolites, in urine or adults and neonates, studied by gas chromatography-mass spectrometry and radioimmunoassay.
Endogenous levels of two metabolites of prostacyclin, 6-keto-prostaglandin F1 alpha (spontaneous hydrolysis product) and 6,15-diketo-13,14-dihydroprostaglandin F1 alpha (enzymatic degradation product) were measured in urine of adults and neonates by gas chromatography-mass spectrometry, using deuterated internal standards. The method comprised extraction of prostanoids by reverse-phase cartridges and purification by silicic acid column chromatography and reverse-and straight -phase high-performance liquid chromatographies. Exogenous 6-keto-prostaglandin F1 alpha and 6,15-diketo-13,14-dihydroprostaglandin F1 alpha added to urine were recovered quantitatively by gas chromatography-mass spectrometry. Endogenous levels of 6-keto-prostaglandin F1 alpha in urine of adults were 0.11 +/- 0.05 (S.D.) ng/ml (n = 12), whereas in urine of neonates the levels were much higher: 1.41 +/- 0.36 (S.D.) ng/ml (n = 5) on the 3rd day of life declining to 0.51 +/- 0.21 (S.D.) ng/ml (n = 5) on the 5th day. 6-keto-prostaglandin F1 alpha was also estimated in both age groups by radioimmunoassay. Urinary levels of 6,15-diketo-13, 14-dihydroprostaglandin F1 alpha in neonates on the 3rd day of life were 2.12 +/- 0.70 (S.D.) ng/ml (n = 4) and declined until the 5th day. In adult urine this metabolite was below the limit of detection (0.20 ng/ml).
D015121:6-Ketoprostaglandin F1 alpha / Q000652:urine* / Q000031:analogs & derivatives; D000328:Adult; D011464:Epoprostenol / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D007231:Infant, Newborn; D011453:Prostaglandins / Q000652:urine*; D011461:Prostaglandins F, Synthetic / Q000652:urine*; D011863:Radioimmunoassay / Q000379:methods
C017239:6,15-diketoprostaglandin F1alpha; D011453:Prostaglandins; D011461:Prostaglandins F, Synthetic; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol