[Immunochemical isolation and characteristics of polyribosomes synthesizing rat serum albumin].
A novel one-step procedure is described for purification of a specific class of polysomes. The analysis of optimal conditions for immunoadsorption of albumin-synthesizing polysomes was carried out. The native albumin-synthesizing polysomes were isolated. This preparation has the sedimentation coefficient similar to that of the class revealed amongst polysomes from rat liver with 125I- and peroxidase-labeled antibodies. We translated albumin-synthesizing polysomes in oocytes. The analysis of the product of translation of the preparation of albumin-synthesizing polysomes shows that the albumin makes up 90% of the exogenous synthesis.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D005260:Female; D007163:Immunosorbent Techniques; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure*; D009865:Oocytes / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats; D012709:Serum Albumin / Q000096:biosynthesis*; D014982:Xenopus laevis
D012709:Serum Albumin
Immunokhimicheskoe vydelenie i kharakteristika poliribosom, sinteziruiushchikh krysinyĭ syvorotochnyi al'bumin.