Insulin degradation in human erythrocyte. Effects of reduced glutathione on insulin degradation by membrane fractions.
In this study the Authors have evaluated the effects of reduced glutathione on insulin-degrading activity of erythrocyte membrane fractions. The GSH increases, in a manner proportional to its concentration, the insulin degradation by intact membranes, solubilized proteins and membranes treated with Triton X-100. The net increase of degradation is greater for intact and extracted membranes than for solubilized proteins. These data suggest that in human erythrocyte membrane exists an enzymatic system GSH-dependent for insulin degradation and that this system is only partially solubilized by Triton X-100 treatment.
D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005978:Glutathione / Q000494:pharmacology*; D006801:Humans; D007328:Insulin / Q000097:blood*; D017830:Octoxynol; D011092:Polyethylene Glycols; D012995:Solubility
D007328:Insulin; D011092:Polyethylene Glycols; D017830:Octoxynol; D005978:Glutathione