Expression in Escherichia coli of biologically active enzyme by a DNA sequence coding for the human plasminogen activator urokinase.
We have isolated clones of Escherichia coli strain K-12 that contain a hybrid pBR322 plasmid having a 4.2-kilobase insert of a DNA transcript of the mRNA of human plasminogen activator, urokinase. The bacterially produced enzyme has properties similar to those of urokinase from human fetal kidney cells. Both enzymes occur in discrete forms ranging from 32,000 to 150,000 daltons in size. They react with antibody to purified urokinase from human kidney cells, bind to a benzamidine-Sepharose column, and induce plasminogen-dependent lysis of a fibrin clot.
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004274:DNA, Recombinant; D010450:Endopeptidases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D006801:Humans; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D013379:Substrate Specificity; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics* / Q000378:metabolism
D004274:DNA, Recombinant; D012333:RNA, Messenger; D010450:Endopeptidases; D014568:Urokinase-Type Plasminogen Activator