Structural features and selective expression of three Ly-5+ cell-surface molecules.
Conventional Ly-5 alloantisera precipitate cell-surface molecules of three sizes: 200K, 205K, and 220K. In SDS-PAGE, the rat monoclonal antibody 74/8' precipitates the same three molecules from both Ly-5.1 and Ly-5.2 cells. Cleveland mapping of the three molecules, precipitated by reaction of conventional Ly-5 alloantisera or 74/8' monoclonal antibody with lysates of 125I-labeled cells, disclosed no differences among the three molecular forms, but markedly distinguished all three Ly-5.1 molecules from all three Ly-5.2 molecules. Each of the three molecular forms can be expressed independently of the other two by cloned culture lines of Ly-5+ cells of different hematopoietic lineage. All of the seven cloned lines tested expressed only one form. However, two of the seven uncloned culture lines tested, plasmacytoma MOPC-70A and the X.1 putative macrophage line which originated from an SJL tumor, yielded both 200K and 205K forms.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000950:Antigens, Ly / Q000032:analysis* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D007163:Immunosorbent Techniques; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology; D008970:Molecular Weight; D011487:Protein Conformation
D000911:Antibodies, Monoclonal; D000950:Antigens, Ly