Regulation of 25 hydroxyvitamin D3 1-hydroxylase in serum-free monolayer culture of mouse kidney.
Primary monolayer cultures of cells from normal mouse kidney were initiated and maintained in serum-free medium supplemented with insulin, transferrin and prostaglandin E1. Renal epithelial cells grow to confluence without detectable growth of fibroblasts. These cells contain an active 25(OH)D3 1-hydroxylase with half-maximal formation of 1,25(OH)2D3 achieved at a substrate concentration of 13.3 nM. Activity of this enzyme is increased by low calcium medium or parathyroid hormone and decreased by high calcium medium, high phosphate medium or 1,25(OH)2D3.
D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase / Q000378:metabolism*; D000527:Alprostadil; D000818:Animals; D001769:Blood; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D007328:Insulin / Q000494:pharmacology; D007668:Kidney / Q000201:enzymology* / Q000502:physiology; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011458:Prostaglandins E / Q000494:pharmacology; D013250:Steroid Hydroxylases / Q000378:metabolism*; D014168:Transferrin / Q000494:pharmacology
D003470:Culture Media; D007328:Insulin; D011458:Prostaglandins E; D014168:Transferrin; D013250:Steroid Hydroxylases; D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase; D000527:Alprostadil