Immunological specificity of Novikoff hepatoma chromatin: isolation of three antigenic proteins.
1. Immunization of rabbits with dehistonized Novikoff hepatoma or normal rat liver chromatin elicited specific antibodies. 2. Chromosomal nonhistone proteins from Novikoff hepatoma were fractionated and three proteins (approx. mol. wt 39,000, 49,0000 and 56,000) were identified with the hepatoma antigenic complexes. 3. All three proteins showed significant reactivity of PAS staining suggesting that they were glycosylated. 4. Proteins migrating similar to these three Novikoff hepatoma fractions were detected by polyacrylamide gel electrophoresis in the chromosomal nonhistone protein fraction obtained from normal rat liver. 5. However, the three proteins from normal liver were PAS negative, and did not react immunologically with antiserum to dehistonized Novikoff hepatoma chromatin.
D000818:Animals; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D002843:Chromatin / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D007118:Immunoassay; D007124:Immunoenzyme Techniques; D008114:Liver Neoplasms, Experimental / Q000276:immunology*; D008297:Male; D009363:Neoplasm Proteins / Q000276:immunology / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains
D000951:Antigens, Neoplasm; D002843:Chromatin; D009363:Neoplasm Proteins