[Effect of protein synthesis inhibitors on D-xylose transport in frog muscle tissue].
The action of puromycin and cycloheximide on the uptake of D-xylose in isolated frog sartorius muscles has been studied. Cycloheximide and puromycin were found to stimulate the basal transport of xylose and to inhibit the insulin-stimulated sugar uptake. On the other hand, both these antibiotics did not affect the sugar transport stimulated by 2,4-dinitrophenol. Both of them, even at a high concentration (up to 0.1 mg/ml), inhibited the incorporation of leucine or valine into muscle proteins by 50% only, whereas they did inhibit the sugar transport at a concentration much lesser by several orders. The inhibitors of protein synthesis are supposed to affect directly the signal transmission from insulin receptors to the sugar transport system.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin; D007329:Insulin Antagonists; D009124:Muscle Proteins / Q000037:antagonists & inhibitors*; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D011691:Puromycin / Q000494:pharmacology; D011896:Rana temporaria; D014994:Xylose / Q000378:metabolism*
D007328:Insulin; D007329:Insulin Antagonists; D009124:Muscle Proteins; D011691:Puromycin; D003513:Cycloheximide; D014994:Xylose
Vliianie ingibitorov belkovogo sinteza na transport D-ksilozy v myshechnoi tkani liagushki.