Localization of the human insulin gene to the distal end of the short arm of chromosome 11.
The human insulin gene was mapped by hybridization in situ of 3H-labeled recombinant plasmid pHIG900 to chromosome preparations in the presence of 10% dextran sulfate. pHIG900 contains a 900-base pair insert of human genomic DNA that includes the coding region for the C peptide and intervening sequence 2. A significant percentage of hybridized cells (34%) exhibited silver grains on the distal end of the short arm (band p15) of chromosome 11. Furthermore, silver grains on this region represented 26% of all chromosomal label. These results demonstrate chromosomal localization of the human insulin gene to 11p15.
D002874:Chromosome Mapping; D002906:Chromosomes, Human, 6-12 and X; D004274:DNA, Recombinant; D005796:Genes; D006801:Humans; D007328:Insulin / Q000235:genetics*; D008214:Lymphocytes / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization
D004274:DNA, Recombinant; D007328:Insulin