Influence of 6-keto-PGF1 alpha on collagen synthesis in the human cervix during various phases of the menstrual cycle.
Uterine cervix tissue, obtained from nonpregnant fertile women undergoing hysterectomy, was mechanically chopped into 1 mm thick slices and incubated in Krebs-Ringer bicarbonate buffer containing 6-keto-PGF1 alpha (0.03-10 microgram/ml) and 3H-proline. After incubation of 30-120 min the incorporated radioactivity was determined and related to the protein content of each slice. 6-keto-PGF1 alpha had specific and significant effects on the incorporation of 3H-proline into cervical tissue. In the follicular phase of the cycle a decreased incorporation was registered, indicating a reduced net synthesis of protein. However, increased radiolabelling was observed in the luteal phase, reflecting an augmented protein synthesis. It is suggested that 6-keto-PGF1 alpha, the stable metabolite of prostacyclin (PGI2), has the ability to influence cervical protein metabolism and that this effect is steroid hormone dependent.
D015121:6-Ketoprostaglandin F1 alpha / Q000494:pharmacology*; D002584:Cervix Uteri / Q000378:metabolism*; D003094:Collagen / Q000096:biosynthesis*; D011464:Epoprostenol / Q000494:pharmacology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008598:Menstruation; D011392:Proline / Q000378:metabolism
D015121:6-Ketoprostaglandin F1 alpha; D003094:Collagen; D011392:Proline; D011464:Epoprostenol