title: Proline biosynthesis in Escherichia coli. Stoichiometry and end-product identification of the reaction catalysed by glutamate semialdehyde dehydrogenase.
abstract: The stoichiometry of the oxidative phosphorylation of glutamic acid 5-semialdehyde by gamma-glutamyl phosphate reductase (glutamate semialdehyde dehydrogenase) has been established. Equimolar amounts of NADP+ and L-glutamic acid 5-semialdehyde are consumed and equimolar amounts of 5-oxiopyrroilidine-2-carboxylic acid and NADPH are formed. The end-product of the reaction is demonstrated to be 5-oxopyrrolidine-2-carboxylic acid, probably arising from the true end-product gamma-glutamyl phosphate.
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000378:metabolism*; D050698:Glutamate-5-Semialdehyde Dehydrogenase; D009249:NADP / Q000378:metabolism; D011392:Proline / Q000096:biosynthesis*; D011761:Pyrrolidonecarboxylic Acid / Q000378:metabolism
chemical_list: D009249:NADP; D011392:Proline; D000445:Aldehyde Oxidoreductases; D050698:Glutamate-5-Semialdehyde Dehydrogenase; D011761:Pyrrolidonecarboxylic Acid