Isolation of 80S ribosomes from spores of Aspergillus fumigatus and their antigenicity in rabbits.
Ribosomes were obtained from spores of Aspergillus fumigatus by mechanical disruption and differential centrifugation. The initial preparation (crude ribosomes) contained spore components which appeared to be broken fragments of plasmalemma with or without organelles. Purified ribosomes free of membranous material were prepared by gel filtration chromatography on Sepharose CL-4B. Monomeric 80S ribosomes consisting of 40% protein and 60% RNA, and morphologically characteristic of fungal ribosomes were isolated. Serological reactions in sera from rabbits with crude or purified ribosomes were similar indicating that the purification process did not change or eliminate antigens that stimulated antibody detectable either by indirect hemagglutination (IHA) or in gel precipitin tests.
D000818:Animals; D000946:Antigens, Fungal / Q000276:immunology*; D001232:Aspergillus fumigatus / Q000648:ultrastructure*; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D005656:Fungal Proteins / Q000032:analysis; D012331:RNA, Fungal / Q000032:analysis; D011817:Rabbits; D012270:Ribosomes / Q000032:analysis / Q000276:immunology* / Q000648:ultrastructure; D013172:Spores, Fungal / Q000648:ultrastructure
D000946:Antigens, Fungal; D005656:Fungal Proteins; D012331:RNA, Fungal