title: Studies on the possible involvement of complement component C3 in the initiation of acid hydrolase secretion by macrophages.
abstract: Complement component C3 has been detected on the plasma membranes of mouse peritoneal macrophages by using an immunoperoxidase technique in conjunction with transmission electron microscopy. Evidence that such cell surface-associated C3 might be the trigger for lysosomal enzyme discharge was sought initially by exposing macrophage monolayers to anti-mouse C3 F(ab')2, and later, by treating cells with the antibody fragment before two potent secretagogues (methylamine and zymosan particles). Both methods, however, failed to demonstrate a role for cell surface-associated C3 in the initiation of enzyme secretion.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D002478:Cells, Cultured; D003176:Complement C3 / Q000032:analysis / Q000502:physiology*; D005966:Glucuronidase / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology; D008264:Macrophages / Q000201:enzymology* / Q000276:immunology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron
chemical_list: D003176:Complement C3; D007770:L-Lactate Dehydrogenase; D005966:Glucuronidase