title: Bacillus licheniformis penicillinase synthesized in Escherichia coli contains covalently linked fatty acid and glyceride.
abstract: DNA sequence analysis of the structural gene for Bacillus licheniformis penicillinase has revealed a tetrapeptide sequence of Leu-Ala-Gly-Cys within the NH2-terminal part of the precursor form of penicillinase (penicillin amido-beta-lactamhydrolase, EC 3.5.2.6). The same tetrapeptide occurs in the signal sequence of the prolipoprotein of Escherichia coli, and the cysteine residue in the tetrapeptide of prolipoprotein is modified to form glyceride-cysteine which becomes the NH2 terminus of Braun's lipoprotein. On the basis of labeling, with [2-3H]glycerol, [3H]palmitate, [35S]methionine, and [35S]sulfuric acid, of an E. coli strain lysogenic for a lambda vector containing the penicillinase gene from B. licheniformis and of immunoprecipitation with rabbit antisera against purified B. licheniformis penicillinase, we conclude that B. licheniformis penicillinase synthesized in E. coli contains covalently linked glyceride and fatty acid. These results strongly suggest the operation of a modification system in E. coli, and presumably in other Gram-negative bacteria, which results in the formation of a glyceride-cysteine residue if the proper peptide sequence is present in the signal sequence of membrane proteins.
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D003545:Cysteine / Q000378:metabolism; D004792:Enzyme Precursors / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005227:Fatty Acids / Q000378:metabolism; D005989:Glycerides / Q000378:metabolism; D008074:Lipoproteins / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism; D010405:Penicillinase / Q000096:biosynthesis*; D010710:Phosphates / Q000378:metabolism; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D004792:Enzyme Precursors; D005227:Fatty Acids; D005989:Glycerides; D008074:Lipoproteins; D008563:Membrane Lipids; D010710:Phosphates; D010405:Penicillinase; D001618:beta-Lactamases; D003545:Cysteine