title: In vitro evaluation of CENTA, a new beta-lactamase-susceptible chromogenic cephalosporin reagent.
abstract: CENTA is a newly synthesized, beta-lactamase-labile, chromogenic cephalosporin reagent which changes color from light yellow (lambda maximum ca. 340 nm) to chrome yellow (lambda maximum ca. 405 nm) concomitant with hydrolysis of the beta-lactam ring. This compound offers promise as a diagnostic reagent comparable to other chromogens (PADAC and nitrocefin) for the early detection of beta-lactamase-producing clinical isolates, while retaining some antimicrobial effect against Escherichia coli, Klebsiella spp., Proteus mirabilis, Staphylococcus aureus, and non-enterococcal Streptococcus spp. CENTA is relatively unaffected by commonly used microbiological media and human serum.
mesh_terms: D001431:Bacteriological Techniques; D002511:Cephalosporins / Q000494:pharmacology; D002954:Citrobacter / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D004755:Enterobacteriaceae / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D005069:Evaluation Studies as Topic; D007202:Indicators and Reagents; D007709:Klebsiella / Q000187:drug effects; D011513:Proteus mirabilis / Q000187:drug effects; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D002511:Cephalosporins; D007202:Indicators and Reagents; D065093:beta-Lactamase Inhibitors; C034313:pyridine-2-azo-4-dimethylaniline cephalosporin; C034025:CENTA; D001618:beta-Lactamases; C021720:nitrocefin