title: The relationship of fibroblast translocations to cell morphology and stress fibre density.
abstract: Translocation of human fibroblasts in culture was studied using techniques of time-lapse cinemicrography, indirect immunofluorescence, and computer analysis. An inverse relationship between the velocity of cells during the last hour of life and the density of stress fibers seen by immune staining was demonstrated. Translocating cells generally assumed one of two interconvertible morphologies: a triangular tailed shape or tailed fibroblast (TF), and a tailless form that resembled a half-moon, which we call a half-moon fibroblast (HMF). The tail of TFs formed only on regions of substrate that had been previously traversed by cells. The half-moon morphology developed either on previously used or on virgin substrate. Cells adopted the HMF rather than the TF morphology with a four-fold greater frequency. HMFs translocated slightly faster than TFs. The foregoing observation suggest that the fibroblast tail is not an organelle essential for translocation. Since our technique allowed us to distinguish between cells which were cycling and those which had left cycle, we compared their velocities and found them to be similar. Also the average velocities of cells of different population-doubling levels (10th, 30th, 40th) were approximately equal.
mesh_terms: D002455:Cell Division; D002465:Cell Movement; D003201:Computers; D003599:Cytoskeleton / Q000648:ultrastructure*; D005347:Fibroblasts / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D006801:Humans; D009040:Motion Pictures; D012867:Skin; D013997:Time Factors