L-Lactate as a source of carbon for fatty acid synthesis in adult and foetal sheep.
The contribution of carbon from acetate, glucose and L-lactate to fatty acid synthesis has been compared in adipose tissue from adult and foetal sheep. Adipose tissue slices were incubated with mixtures of acetate, glucose and L-lactate at concentrations found in sheep blood. Under these conditions acetate, glucose and L-lactate contributed 95, 2 and 3%, respectively, of the C2 units used for fatty acid synthesis in adipose tissue slices from adult sheep. In contrast, acetate, glucose and L-acetate provided 50, 17 and 33%, respectively, of the C2 units for fatty acid synthesis in adipose tissue slices from foetal lambs (about 30 days prior to birth). The contribution of acetate and L-lactate carbon to fatty acid synthesis varied with their concentrations over the normal physiological range at both ages. We conclude that L-lactate is normally a minor source of carbon for fatty acid synthesis in the adult sheep but is an important source in foetal lambs.
D000085:Acetates / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D005227:Fatty Acids / Q000096:biosynthesis*; D005260:Female; D005333:Fetus; D005947:Glucose / Q000378:metabolism; D007773:Lactates / Q000378:metabolism*; D019344:Lactic Acid; D011247:Pregnancy; D011865:Radioisotope Dilution Technique; D012756:Sheep
D000085:Acetates; D002250:Carbon Radioisotopes; D005227:Fatty Acids; D007773:Lactates; D019344:Lactic Acid; D005947:Glucose