Fate of two 14C labelled muramyl peptides: Ac-Mur-L-Ala-gamma-D-Glu-meso-A2pm and Ac-Mur-L-Ala-gamma-D-Glu-meso-A2pm-D-Ala-D-Ala in mice. Evaluation of their ability to increase non specific resistance to Klebsiella infection.
The metabolic fate in mice of two 14C labelled meso-A2pm containing muramyl-peptides, the muramyl-tripeptide (Ac-Mur-L-Ala-gamma-D-Glu-14C-meso-A2pm) (MTP) and the muramyl-pentapeptide (Ac-Mur-L-Ala-gamma-D-Glu-meso-A2pm-14C-D-Ala-14C-D-Ala) (MPP) has been studied. As with 14C-MDP, the radioactive muramyl-tripeptide and muramyl-pentapeptide disappear rapidly from the organs and the radioactivity is found mainly in the urine. In contrast to MDP, the two meso-A2pm containing muramyl-peptides are not excreted intact in the urine. In both cases labelled fragments have been identifed: meso-A2pm from MTP and the tetrapeptide gamma-D-Glu-meso-A2pm-D-Ala-D-Ala from MPP. The ability of the two muramyl-peptides to increase nonspecific resistance of mice to Klebsiella infection was also investigated. The muramyl-pentapeptide injected i.v. one day before a lethal dose of K. pneumoniae protects both adult and neonate mice, as does MDP itself; the muramyl-tripeptide is inactive.
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D006020:Glycopeptides; D007109:Immunity / Q000187:drug effects; D007275:Injections, Intravenous; D007668:Kidney / Q000378:metabolism; D007710:Klebsiella Infections / Q000276:immunology*; D007711:Klebsiella pneumoniae / Q000276:immunology; D051379:Mice; D013268:Stimulation, Chemical; D014018:Tissue Distribution
D006020:Glycopeptides; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C035104:muramylNAc-Ala-isoGln-Lys-tripeptide; C035105:AcMu-Ala-iso-Gln-meso-2,2'-diaminopimelic acid-Ala-Ala