Intracellular localization of antigens with backscatter mode of SEM using peroxidase-labeled antibodies.
The peroxidase-labeled antibody method was employed for immunohistochemical localization of intracellular and extracellular antigens with SEM. With this method, the antigenic sites may be localized with high sensitivity on tissue sections mounted on carbon-coated glass slides by detecting 0s04-DAB complexes using the backscatter mode. The sections may be fresh frozen fixed on slide, frozen sections of fixed tissue, or sections of immunostained tissue embedded in epon. The method introduces another utilization of SEM and the immunoenzyme histochemistry.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D003584:Cytological Techniques; D005404:Fixatives; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000276:immunology / Q000648:ultrastructure; D007684:Kidney Tubules / Q000648:ultrastructure; D051379:Mice; D008855:Microscopy, Electron, Scanning; D012542:Scattering, Radiation
D000941:Antigens; D005404:Fixatives