[Study of platelet aggregation by the filtration pressure method (author's transl)].
Using Hornstra's "filter loop" technique, effects of prostaglandins and vasodilators on ADP-induced platelet aggregation were studied in anaesthetized dogs. ADP-induced platelet aggregation was observed as a transient, reversible elevation of pressure before filter. This pressure change was reproducible and dose-related. PGI2 and PGE1 suppressed the elevation of pressure induced by ADP and decreased the spontaneous elevation of pressure. Thus, it was reconfirmed that PGI2 and PGE1 not only suppressed ADP-induced platelet aggregation but also produced a disaggregation of aggregated platelets. AFter indomethacin, ADP-induced platelet aggregation was potentiated and sometimes became exceedingly protracted, indicating that anti-aggregatory prostaglandin was always present in the circulating blood. Close-injection of bradykinin did not suppress ADP-induced platelet aggregation, while intravenous injection did produce a suppression. After indomethacin, this inhibitory effect was greatly attenuated, indicating a release of anti-aggregatory prostaglandin (PGI2?) by bradykinin. Coronary vasodilators, such as dipyridamole and nifedipine did not inhibit ADP-induced platelet aggregation, nor did sulfinpyrazone. It is concluded that the "filter loop" technique is a useful method that allows aggregation and disaggregation reactions to be followed continuously and quantitatively in the blowing blood. The effects of drugs on platelet aggregation can be assessed in connection with hemodynamic changes such as blood pressure and heart rate induced by systemic administration of the drugs.
D000244:Adenosine Diphosphate / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000527:Alprostadil; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D004285:Dogs; D011464:Epoprostenol / Q000494:pharmacology; D005260:Female; D005374:Filtration / Q000379:methods; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D010976:Platelet Count; D011312:Pressure; D011458:Prostaglandins E / Q000494:pharmacology; D013442:Sulfinpyrazone / Q000494:pharmacology; D014665:Vasodilator Agents / Q000494:pharmacology
D011458:Prostaglandins E; D014665:Vasodilator Agents; D000244:Adenosine Diphosphate; D011464:Epoprostenol; D000527:Alprostadil; D001920:Bradykinin; D013442:Sulfinpyrazone; D007213:Indomethacin