Measurement of specific multiplication of an oncolytic and a nononcolytic strain of Clostridium butyricum inside the tumour UVT 15264 of the mouse.
Clostridium butyricum was recultivated from tumour material after treatment of tumour-bearing mice with spores and counted by soft agar colony technique. The oncolytic strain Cl. butyricum H8 grows inside the tumour UVT 15264 up to 8 X 10(8)/g during 48 h. In this time part of tumour became lysed. Multiplication inside of this tumour by the tumour specific but nononcolytic strain Cl. butyricum CNRZ 528 could not be detected by application counting technique. Combination of spore treatment with antibiotic chemotherapy in dosage able to prevent oncolysis or in dosage to reduce them corresponds with loss of clostridial multiplication or with reduced vegetative multiplication respectively.
D000818:Animals; D001431:Bacteriological Techniques; D003013:Clostridium / Q000187:drug effects / Q000254:growth & development*; D004338:Drug Combinations / Q000494:pharmacology; D051379:Mice; D009369:Neoplasms; D010400:Penicillin G / Q000494:pharmacology; D010401:Penicillin G Benzathine / Q000494:pharmacology; D010402:Penicillin G Procaine / Q000494:pharmacology; D012513:Sarcoma, Experimental / Q000382:microbiology*; D012878:Skin Neoplasms / Q000382:microbiology*; D013045:Species Specificity; D013171:Spores, Bacterial / Q000187:drug effects / Q000254:growth & development
D004338:Drug Combinations; C024657:benzathine benzylpenicillin, procaine benzylpenicillin, drug combination; D010402:Penicillin G Procaine; D010400:Penicillin G; D010401:Penicillin G Benzathine