Demonstration of JC virus by immunofluorescence in multiple cell types in experimentally infected adult human brain cell cultures.
JC virus (JCV)-induced T antigen developed in the nuclei of approximately 50% of adult human brain (AHB) cells infected with JCV. Approximately 10% of these T antigen-positive cells contained glial fibrillary acidic protein (GFAP) and were identified as astrocytes in immunofluorescence studies. About 50% of these T antigen-positive cells bound antiserum to fibronectin on their surface and were identified as fibroblasts. No cells binding antiserum to galactocerebroside (GC), an oliodendrocyte-specific marker, were detected in infected or non-infected AHB cultures. the cell type of AHB that constitutes the remaining 40-50% of T antigen-positive cells that are GFAP-, fibronectin- and GC-negative remains to be determined.
D000328:Adult; D000941:Antigens / Q000276:immunology; D000955:Antigens, T-Independent / Q000276:immunology; D001253:Astrocytes / Q000276:immunology; D001921:Brain / Q000276:immunology*; D046508:Culture Techniques; D005347:Fibroblasts / Q000276:immunology; D005353:Fibronectins / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005699:Galactosylceramides / Q000276:immunology; D005904:Glial Fibrillary Acidic Protein; D006801:Humans; D009419:Nerve Tissue Proteins / Q000276:immunology; D027383:Papillomaviridae / Q000276:immunology*; D027382:Polyomaviridae
D000941:Antigens; D000955:Antigens, T-Independent; D005353:Fibronectins; D005699:Galactosylceramides; D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins