The haemolytic antibody isotope release (HAIR) assay: an efficient alternative technique to conventional plaque assays.
The haemolytic antibody isotope release (HAIR) assay quantitates antibody production by splenic antibody-producing cells by lysis of chromium-51-labelled sheep red blood cells. The amount of antibody quantitated by the HAIR assay directly correlates with the number of antibody-producing cells measured by a conventional plaque assay. The HAIR assay is an easy, sensitive, and reproducible technique that is especially useful when large numbers of animals are required for testing.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D002860:Chromium Radioisotopes; D003165:Complement System Proteins; D005260:Female; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D006461:Hemolysis; D006462:Hemolytic Plaque Technique; D007158:Immunologic Techniques; D008297:Male; D051379:Mice; D013154:Spleen / Q000276:immunology
D002860:Chromium Radioisotopes; D006460:Hemolysin Proteins; D003165:Complement System Proteins