Re-inoculation of surface material from culture negative medium as an aid to the isolation of mycobacteria.
Re-inoculation by a spiral loop of surface material from culture negative medium on to fresh egg medium gave positive growth of mycobacteria in 3 out of 521 sputum specimens.
D001431:Bacteriological Techniques; D009161:Mycobacterium / Q000302:isolation & purification*; D013183:Sputum / Q000382:microbiology*