Regulation of A- and B-cell function by insulin and glucagon.
In monolayer cultures of pancreases from neonatal rats, glucagon added to the medium (5 or 50 ng/ml) stimulated insulin secretion. In contrast, exogenously added insulin (1.0 mU/ml) inhibited glucagon secretion when glucose was 1.7 or 5.5 mM, but not when glucose was 16.5 mM. This suggests that insulin may reduce glucagon release by enhancing glucose uptake by A-cells. Suppression of glucagon secretion by high glucose (16.5 mM) may be mediated by endogenously released insulin. These observations support the in vivo findings favoring a modulatory interaction between A- and B-cells.
D000818:Animals; D002478:Cells, Cultured; D005934:Glucagon / Q000378:metabolism / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D005934:Glucagon; D005947:Glucose