T cell responses to alloantigens. III. Prolonged circulation of specific cytotoxic T memory cells following skin allograft rejection in mice.
A limit dilution technique was used to study specific circulating cellular memory to alloantigens in the blood of B6AF1 mice that had rejected B10.D2 or B10.BR skin grafts. A specific increase in cytotoxic T cell (Tc) precursor (CTL-P) frequency was detected as the skin grafts were rejected, and this persisted for the duration of the experiment (8 months) in the B6AF1 mice that had rejected B10.D2 skin grafts. By contrast, the CTL-P frequency for H-2Dk in the blood of B6AF1 mice that had rejected B10.BR skin grafts had returned to normal by 4 months after graft rejection. In addition, it was shown that peripheral blood lymphocytes from these immune mice could respond to heat-inactivated spleen cells of donor origin, whereas those of naive mice could not.
D000818:Animals; D003602:Cytotoxicity, Immunologic; D006084:Graft Rejection; D007156:Immunologic Memory; D007519:Isoantigens / Q000276:immunology*; D051379:Mice; D016038:Skin Transplantation; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D007519:Isoantigens