Comparative study on the effects of cyclophosphamide on yeast in vitro and in the host-mediated assay: DNA damage and biological response.
Cyclophosphamide (CP), whether applied in its chemically activated form as 4-hydroperoxy-cyclophosphamide (4-OOH-CP) in vitro or in the host-mediated assay (HMA) using rats, exhibits toxic and mutagenic effects on excision deficient yeast cells. The expression of these effects is examined during a prolonged postincubation in buffer and compared with the ability of activated CP to induce interstrand cross-links and DNA fragmentation. At comparable doses, we observed a close similarity of biological and biochemical effects in either test system.
D000818:Animals; D002500:Centrifugation, Isopycnic; D003520:Cyclophosphamide / Q000031:analogs & derivatives* / Q000633:toxicity*; D004271:DNA, Fungal / Q000378:metabolism*; D009152:Mutagenicity Tests; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation / Q000187:drug effects; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000187:drug effects*
D004271:DNA, Fungal; D003520:Cyclophosphamide; C011272:perfosfamide