Immune complex-mediated lung injury produced by horseradish peroxidase (HRP) and anti-HRP antibodies in rats.
Horseradish peroxidase (HRP) was administered intratracheally to rats with circulating autologous or heterologous anti-HRP antibodies. Intrapulmonary immune complexes containing HRP, anti-HRP antibodies, but no C3, were detected by immunofluorescence from 1 hour to 48 hours with a maximum of 6 hours after antigenic challenge. The concomitant hemorrhagic alveolitis characterized by fibrin deposits and infiltration by polymorphs and macrophages was also maximal after 6 hours. At the ultrastructural level, HRP was easily detected, and HRP-containing immune deposits were demonstrated along the basement membrane in subepithelial and subendothelial localization and in interstitial spaces. These findings suggest a C3 independent mechanism for lung injury mediated by immune complexes formed in situ.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006735:Horseradish Peroxidase / Q000276:immunology / Q000378:metabolism; D007668:Kidney / Q000032:analysis; D008168:Lung / Q000032:analysis / Q000648:ultrastructure; D055370:Lung Injury; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000032:analysis
D000906:Antibodies; D000936:Antigen-Antibody Complex; D006735:Horseradish Peroxidase