Affinity labelling of yeast phenylalanyl-tRNA synthetase with a 3'-oxidised tRNAPhe. Isolation and sequence of the labelled peptide.
Yeast phenylalanyl-tRNA synthetase was specifically labelled with a 3'-oxidised tRNAPhe. Stoichiometric inactivation was achieved with the incorporation of 2 mol oxidised tRNA Phe/mol enzyme which corresponds exactly to the stoichiometry of tRNA binding. The labelled peptide has been isolated using a quick chromatographic procedure which can be applied to any covalent complex formed between a tRNA and an aminoacyl tRNA synthetase. The isolated peptide (18 amino acids) was found to encompass the unique cysteine sequence of the smaller beta subunit of the enzyme.
D000345:Affinity Labels; D000596:Amino Acids / Q000032:analysis; D000604:Amino Acyl-tRNA Synthetases; D055598:Chemical Phenomena; D002621:Chemistry; D003545:Cysteine / Q000032:analysis; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000302:isolation & purification; D010447:Peptide Hydrolases; D010652:Phenylalanine-tRNA Ligase; D012346:RNA, Transfer, Amino Acyl; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012697:Serine Endopeptidases; D013820:Thermolysin; D014357:Trypsin
D000345:Affinity Labels; D000596:Amino Acids; D010446:Peptide Fragments; D012346:RNA, Transfer, Amino Acyl; D010447:Peptide Hydrolases; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D014357:Trypsin; D013820:Thermolysin; D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase; D003545:Cysteine