Cell competence for industion of differentiation by insulin and other compounds in myeloid leukemic clones continuously cultured in serum-free medium.
Clones of myeloid leukemic cells varying in their competence for induction of differentiation have been continuously grown in serum-free medium. In the medium used, which contained transferrin, the growth rates of these cells were nearly similar to those found in serum-containing medium. The clones also maintained in this medium their competence for induction of differentiation by the normal macrophage and granulocyte differentiation-induction protein MGI-2, the steroid dexamethasone, and lipopolysaccharide. In contrast to the results with these inducters, some clones continuously cultured in a serum-free medium showed a gain of inducibility by insulin and another clone a gain of inducibility by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate in low serum and serum-free medium. Induction of differentiation by these two compounds was therefore inhibited in these clones by the presence of serum. It is suggested that serum-free medium may also show the existence of other inducers of differentiation not detected in serum-containing medium and that these results are relevant to the possible therapeutic use of compounds such as insulin for the induction of normal differentiation in leukemic cells in vivo.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D002999:Clone Cells / Q000187:drug effects; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D003470:Culture Media; D003907:Dexamethasone / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology; D007328:Insulin / Q000494:pharmacology*; D007951:Leukemia, Myeloid / Q000097:blood*; D051379:Mice; D008815:Mice, Inbred Strains; D009113:Muramidase / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014168:Transferrin / Q000494:pharmacology
D003115:Colony-Stimulating Factors; D003470:Culture Media; D007328:Insulin; D014168:Transferrin; D003907:Dexamethasone; D009113:Muramidase; D013755:Tetradecanoylphorbol Acetate