Initial evaluation of a convection counter streaming galvanization technique of sex separation of human spermatozoa.
A new method for separating X and Y human spermatozoa called convection counter streaming galvanization was evaluated. The method was independently performed by this semenology laboratory with the use of the special separation equipment and extending media provided by its developer, Dr. Bhairab C. Bhattacharya. The mean number of Y spermatozoa increased from 48% to 77% in the separated fraction predicted to be Y-enriched. The fraction predicted to be X-enriched increased from a mean of 52% to 77%. The one separation process allowed accumulation of both enriched fractions simultaneously. The separated portions of spermatozoa maintained good motility and penetration of cervical mucus but produced a mean recovery concentration in the X- and Y-enriched fractions of only 15% to 16% of the preseparation concentration.
D002469:Cell Separation / Q000379:methods*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D012730:Sex Chromosomes; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology*; D014960:X Chromosome; D014998:Y Chromosome