Preparation of small temporal bones for high-resolution light microscopy.
A technique was used for rapid processing of small temporal bones for high-resolution light microscopy, Temporal bones were decalcified in edetic acid and embedded in glycol methacrylate. Sections (thickness, 1 micrometer) were stained with a variety of stains that demonstrated cellular details not usually seen with conventional methods. This technique is suitable for routine histologic, histochemical, and radioautographic studies of small temporal bones.
D000818:Animals; D003648:Decalcification Technique; D007758:Ear, Inner / Q000166:cytology; D004432:Ear, Middle / Q000166:cytology; D005849:Gerbillinae; D006652:Histological Techniques; D051379:Mice; D013701:Temporal Bone / Q000166:cytology*