title: [High-immunogenic mutants of Salmonella with two independently of each other attenuating markers as potential vaccines from bacteria capable of multiplication. I. communication: usefulness of the purin-auxotrophy for the isolation of high-immunogenic and stable double-marker strains (author's transl)].
abstract: The Salmonella example demonstrates a principle for the isolation of high-immunogenic, stable Salmonella-mutants with two independently of each other attenuating mutations as potential vaccines from bacteria capable of multiplication. The isolation of such double-marker vaccination-strains is verified by the treatment of a single-marker strain (for instance an attenuated high-immunogenic auxotrophic pheno-type) with mutagen and the following selection of clones with the marker purin-auxotrophy as a second attenuating mutation. The demonstrated double-marker strains S. typhimurium his-155 pur-4 and S. dublin met-91 pur-23 are designated by the following parameters: Stability under the conditions of the practical vaccine application; immunogenicity for mice by one immunization only, and separability from homologous wild strains with simple laboratory methods.
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines; D005819:Genetic Markers; D051379:Mice; D008813:Mice, Inbred ICR; D009154:Mutation; D011687:Purines / Q000378:metabolism; D012475:Salmonella / Q000235:genetics / Q000276:immunology*; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology*; D014611:Vaccination
chemical_list: D001428:Bacterial Vaccines; D005819:Genetic Markers; D011687:Purines
vernacular_title: Hochimmunogene Salmonella-Mutanten mit zwei unabhängig voneinander attenuierenden Markern als potentielle Impfstoffe aus vermehrungsfähigen Bakterien. I. mitteilung: Eignung der Purin-Auxotrophie für die Herstellung hochimmunogener, stabiler Doppelmarker-Stämme.