[Establishment of the endocrine function of cultured beta cells of the islets of Langerhans].
Monolayer cultures of pancreatic islets obtained from the pancreas of guinea-pigs aged 10 days and 3-3.5-months pig embryos were studied at the 2d-3d, 5th-6th and 15th-16th days. These times corresponded with the stages of culture intense growth, stabilization and physiological dying off. The rate of insulin biosynthesis and secretory activity of beta-cells were evaluated from the intensity of 3H-uridine and 3H-leucine incorporation and from the magnitude of immunoreactive insulin secretion into the cultural medium. The proliferating and insulin-synthetizing beta-cells were detected by selective 3H-thymidine or 3H-leucine incorporation. In the stages of culture intense growth and dying off the viable beta-cells were distributed into insulin-synthesizing and proliferating ones. During the transition to the stabilization stage the proliferative level of beta-cells drastically decreased, while the biosynthesis and insulin secretion rates increased. This manifested in the increased number of beta-cells which intensely incorporated 3H-thymidine into the nucleus, and 3H-leucin into the cytoplasm, as well as in the rise of immunoreactive insulin concentration in the culture.
D000818:Animals; D002452:Cell Count; D002478:Cells, Cultured; D006168:Guinea Pigs; D007328:Insulin / Q000378:metabolism*; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D013552:Swine / Q000196:embryology; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014529:Uridine / Q000378:metabolism
D007328:Insulin; D007930:Leucine; D013936:Thymidine; D014529:Uridine
O stanovlenii éndokrinnoi funktsii kul'tiviruemykh beta-kletok ostrovkov Langergansa.