Insulin inhibition of calcium binding by human placental membrane.
Insulin and its analogues displaced membrane-bound calcium within a physiological range of insulin concentration, in proportion to both biological potency and ability to displace porcine 125 I-labelled insulin from the insulin receptor. Mild tryptic digestion of the membrane reduced insulin binding but did not reduce specific calcium binding. Displacement of membrane-bound calcium by insulin was dependent on insulin binding to its intact receptor. These studies suggest that Ca2"ay exert a controlling influence on insulin-receptor binding in vivo.
D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008566:Membranes / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011972:Receptor, Insulin / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology
D007328:Insulin; D011972:Receptor, Insulin; D014357:Trypsin; D002118:Calcium