[An enzymatic method for the determination of Hg++ content].
An enzymatic method for determination of Hg++ concentration is suggested. This method is based on the strong inhibitory effect of Hg++ on alcoholdehydrogenase (ADH). A correlation between per cent inhibition of ADH and [Hg++]/[ADH] was found. It was possible to determine [Hg++] in a range of values of [Hg++]/[ADH] from 1,58 to 72. A minimum Hg++ concentration of 3,3 X 10(-9) M was dosed.
D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors; D008628:Mercury / Q000032:analysis*; D008832:Microchemistry / Q000379:methods
D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; D008628:Mercury
Proposta di un metodo enzimatico per la determinazione del contenuto di Hg++.