Synthesis and assembly of hepatitis B virus surface antigen particles in yeast.
The surface antigens of hepatitis B virus (HBsAg) has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5'-flanking region of yeast alcohol dehydrogenase I as a promotor to transcribe surface antigen coding sequences. The protein synthesized in yeast is assembled into particles having properties similar to the 22-nm particles secreted by human cells.
D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000235:genetics; D055598:Chemical Phenomena; D002621:Chemistry; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004279:DNA, Viral; D005786:Gene Expression Regulation; D006510:Hepatitis B Antibodies / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D009876:Operon; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D004279:DNA, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase