Ventral bladder hernia facilitates study of urinary tract infections in rats.
Previous studies have demonstrated that after intravesical inoculation of Escherichia coli, rats drinking 5% glucose-water remained bacteriuric for up to 21 days while rats drinking tap water became abacteriuric within a few days. To facilitate accurate monitoring of bacteriuria, we created a ventral bladder hernia for percutaneous aspiration of urine. After intravesical inoculation with 10(8) E. coli, rats with ventral bladder hernias demonstrated clearance of bacteria at a rate comparable to that observed in rats with intrapelvic bladders (p less than 0.01). Of rats drinking tap water, 6 of 7 (85%) with intrapelvic bladders and 8 of 9 (89%) with ventral hernias had less than 10 colony forming units per ml of urine within 9 days of inoculation. Of rats drinking 5% glucose-water, 4 of 5 (80%) with intrapelvic bladders and 6 of 8 (75%) with ventral bladder hernias had greater than 10(5) colony forming units per ml of urine 9 days after inoculation. The results suggest that this technique does not alter the antibacterial response of control or polyuric rats to E. coli inoculated intravesically.
D000818:Animals; D003114:Colony-Forming Units Assay; D004326:Drinking; D004926:Escherichia coli; D006555:Hernia, Ventral; D008297:Male; D008722:Methods; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D001745:Urinary Bladder Diseases; D014552:Urinary Tract Infections / Q000382:microbiology / Q000652:urine*