Positive immunoselection using antibody-enzyme complexes.
We have developed an immunoselection technique using catalase-anti-catalase complexes coupled to specific antibodies to protect antigen positive target cells from the lethal effects of H2O2. The antibody-enzyme complexes are bound to the target cells through an antibody bridge with specificity for both the complexes and an anti-target cell antibody. In a model system, Thy-1 positive (RL male 1-3) cells were protected by incubation with rabbit anti-brain-associated theta antigen (BAT), sheep anti-rabbit IgG [F(ab)'2] and catalase-anti-catalase complexes. The amount and composition of the complexes adhering to the cells were measured by dual radiolabeling of the catalase and anti-catalase immunoglobulin. This technique provides a means of identifying and isolating large numbers of cells bearing any antigen for which specific antisera are available.
D000818:Animals; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D001666:Binding Sites, Antibody; D001921:Brain / Q000276:immunology; D002374:Catalase / Q000276:immunology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007106:Immune Sera / Q000276:immunology; D007140:Immunoglobulin Fab Fragments; D007158:Immunologic Techniques; D007519:Isoantigens; D007700:Kinetics; D051379:Mice; D011817:Rabbits; D051381:Rats; D012756:Sheep
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007519:Isoantigens; D006861:Hydrogen Peroxide; D002374:Catalase