Direct high-resolution nuclear magnetic resonance studies of cation transport in vivo, Na+ transport in yeast cells.
A new nuclear magnetic resonance (NMR) method for monitoring transmembrane metal cation transport is reported. It is illustrated with a study of Na(+) efflux from Na(+)-rich yeast cells. The technique involves the use of an anionic paramagnetic shift reagent, present only outside the cells, to induce a splitting of the sodium-23 NMR peak, in this case, into components representing intra- and extracellular Na(+). The time course of the efflux is in good agreement with the literature and can be well fitted with a double exponential decay expression. Splitting of the lithium-7 NMR signal from a suspension of Li(+)-rich respiratory-deficient, petite yeasts is also reported.
D001693:Biological Transport, Active; D008094:Lithium; D009682:Magnetic Resonance Spectroscopy; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D013997:Time Factors
D008094:Lithium; D012964:Sodium