Spectral properties of cytochrome b-561 and cytochrome b-565 in mucidin-resistant mutants of Saccharomyces cerevisiae.
The oxidation of NADH in submitochondrial particles isolated from MUC1, MUC2 and MUC3 mucidin-resistant mutants of Saccharomyces cerevisiae is specifically resistant to mucidin. Extra reduction of cytochrome b-565 induced by mucidin is demonstrated in all tested mucidin-resistant mutants. Red shift of cytochrome b-561 is induced by mucidin in two independent MUC3 mutants. In MUC1 and MUC2 mutants, the red shift is not induced by mucidin, while that promoted by antimycin A and 2-n-heptyl-4-hydroxyquinoline N-oxide are normal. It is concluded that the extra reduction of cytochrome b-565 and the red shift of cytochrome b-561 elicited by mucidin can be largely dissociated from the overall inhibition of the electron flow by distinct mucidin-resistant mutations in different exons of the split mitochondrial gene of cytochrome b.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D003573:Cytochrome b Group; D003580:Cytochromes / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D005231:Fatty Acids, Unsaturated; D008689:Methacrylates; D008928:Mitochondria / Q000032:analysis; D009154:Mutation; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D013057:Spectrum Analysis / Q000379:methods; D000073739:Strobilurins
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D003573:Cytochrome b Group; D003580:Cytochromes; D005231:Fatty Acids, Unsaturated; D008689:Methacrylates; D000073739:Strobilurins; D009243:NAD; C023646:cytochrome b561; C008556:mucidin; C027737:cytochrome b565