Purification of superoxide dismutases from human placenta using immunoadsorbent columns.
Copper and zinc containing-superoxide dismutase (Cu,Zn-SOD), and manganese containing-superoxide dismutase (Mn-SOD) were purified form human placenta with immunoadsorbent columns using anti-human SOD-antibody conjugated Sepharose 4B. The final preparation has a high purity with a good recovery. The Cu,Zn-SOD purified by an immunoadsorbent column had less zinc content and was more labile than the native Cu,Zn-SOD, but this difference disappeared completely by dialyzing the enzyme obtained by immunoadsorbent column against the buffer containing 1mM ZnCl2. Other enzymological properties of two SODs were identical with that of native SODs.
D003300:Copper / Q000032:analysis; D004355:Drug Stability; D005260:Female; D006358:Hot Temperature; D006801:Humans; D007163:Immunosorbent Techniques / Q000295:instrumentation; D008345:Manganese / Q000032:analysis; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D013482:Superoxide Dismutase / Q000302:isolation & purification*; D015032:Zinc / Q000032:analysis
D008345:Manganese; D003300:Copper; D013482:Superoxide Dismutase; D015032:Zinc