Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.
A method is described for the affinity purification of antibodies using protein samples that have been electrophoretically transferred to diazotized paper. Using differentiated neuroblastoma cells as the protein sample and a heterogeneous anti-microtubule protein serum, antibodies were isolated that specifically bound only to tubulin on blots and that stained microtubule networks in cells. The general utility of this method for various types of applications is discussed.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D002460:Cell Line; D006224:Cricetinae; D003979:Diazonium Compounds; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D007202:Indicators and Reagents; D051379:Mice; D008870:Microtubules / Q000276:immunology; D009447:Neuroblastoma; D010209:Paper; D014404:Tubulin / Q000276:immunology*
D000906:Antibodies; D003979:Diazonium Compounds; D007202:Indicators and Reagents; D014404:Tubulin; C030003:diazophenylthioether