Evaluation of four assay methods for determination of tobramycin in human serum.
Four assay procedures for tobramycin in serum--enzyme immunoassay (I), substrate-labeled fluorescent immunoassay (II), radioimmunoassay (III), and bioassay (IV)--were compared and evaluated by replicate and analytical recovery studies. I and II were about 50% more precise than III and IV. II was substantially more nearly accurate than the other methods and also gave the best reproducibility (correlation coefficient 0.992 between-day). The least expensive method was IV. Ease of handling favored I and II. Overall, we find II to be the most acceptable procedure for use in the clinical laboratory.
D000900:Anti-Bacterial Agents / Q000097:blood*; D001681:Biological Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay; D012016:Reference Values; D013997:Time Factors; D014031:Tobramycin / Q000097:blood*
D000900:Anti-Bacterial Agents; D014031:Tobramycin