Development of an enzyme-linked immunosorbent assay (ELISA) for hepatitis B e antigen and antibody.
Horseradish peroxidase has been conjugated with human immunoglobulin containing antibody to hepatitis B e antigen (anti-HBe). This was used in a solid-phase 'sandwich' enzyme-linked immunosorbent assay designed for reading by eye, to test sera for HBeAg and anti-HBe. Optimum conditions for preparing the conjugate and for performing the test are described. Results of testing hepatitis B surface antigen (HBsAg)-positive sera were compared with those obtained using a sensitive solid-phase radioimmunoassay. The enzyme assay provided a simple and sensitive method of testing for HBeAg and anti-HBe, and correlated well with the radioimmunoassay.
D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006511:Hepatitis B Antigens / Q000032:analysis*; D006513:Hepatitis B e Antigens / Q000032:analysis*; D006735:Horseradish Peroxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G; D011863:Radioimmunoassay; D012217:Rheumatoid Factor
D000914:Antibodies, Viral; D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens; D006513:Hepatitis B e Antigens; D007074:Immunoglobulin G; D012217:Rheumatoid Factor; D006735:Horseradish Peroxidase