title: [3H-Methyl]-methionine as possible methyl donor for formation of 5'-terminus of in vitro synthesized mRNA of cytoplasmic polyhedrosis virus of silkworm Bombyx mori.
abstract: The mRNA of the cytoplasmic polyhedrosis virus of silkworm Bombyx mori could be synthesized in vitro through the action of the virion associated RNA-polymerase in the presence of [3H-methyl]-methionine instead of S-adenosyl-L-methionine. The 3H-mRNA is isolated from the reaction mixture through a column of DEAE-Sephadex A-25 and digested with nuclease p1 and snake venom phosphodiesterase. The results of paper electrophoresis show that the methyl group of methionine is incorporated into the 5'-terminus of CPV mRNA. Even in the presence of S-adenosyl-L-methionine the [3H-methyl]-methionine could still function as a methyl donor for the formation of the 5'-caps of the mRNA of CPV.
mesh_terms: D000818:Animals; D012831:Bombyx / Q000382:microbiology*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004590:Electrophoresis, Paper; D007304:Insect Viruses / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D012436:S-Adenosylmethionine / Q000378:metabolism; D014771:Virion / Q000201:enzymology
chemical_list: D012333:RNA, Messenger; D012436:S-Adenosylmethionine; D008715:Methionine; D012321:DNA-Directed RNA Polymerases