[Selection criteria and results of medium-term storage of corneas for keratoplasty (author's transl)].
A double-staining technique allowing calculation of the percentage of dead endothelial cells in corneas during medium-term storage is described. A positive correlation was found between the thickness of the cornea and the time since death, and between the percentage of dead endothelial cells and the thickness of the cornea. A negative correlation existed between age and cellular density of the endothelium. Conclusions of these data provides practical applications for the selection of corneas for keratoplasty.
D000293:Adolescent; D000328:Adult; D000368:Aged; D002452:Cell Count; D002470:Cell Survival; D003315:Cornea / Q000166:cytology* / Q000473:pathology; D016039:Corneal Transplantation; D004727:Endothelium / Q000166:cytology / Q000473:pathology; D006801:Humans; D008875:Middle Aged; D013997:Time Factors; D014021:Tissue Preservation; D014343:Trypan Blue
D014343:Trypan Blue
Critères de sélection et résultats de la conservation à moyen terme des cornées destinées à la kératoplastie.