Impaired sensitivity to insulin of rat livers perfused with blood of diminished haematocrit.
1. In livers from fed rats perfused with homologous whole blood of a haematocrit value of 37%, insulin decreased the perfusate concentrations of glucose and amino acids, production of ketone bodies (3-hydroxybutyrate + acetoacetate) and increased bile flow. 2. Perfusion with blood diluted with buffer to a haematocrit value of 17% decreased hepatic O2 consumption by 40-50%. Perfusate concentrations of glucose and lactate, the rate of ketogenesis and the ratios [lactate]/[pyruvate] and [3-hydroxybutyrate]/[acetoacetate] were all increased. 3. In livers perfused with blood of diminished haematocrit, effects of insulin on perfusate glucose an amino acids, ketogenesis and bile flow were abolished.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001790:Blood Physiological Phenomena; D005947:Glucose / Q000378:metabolism; D006400:Hematocrit; D007328:Insulin / Q000494:pharmacology*; D007773:Lactates / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D010477:Perfusion; D011773:Pyruvates / Q000378:metabolism; D051381:Rats
D000596:Amino Acids; D007328:Insulin; D007773:Lactates; D011773:Pyruvates; D005947:Glucose