[Alkylation of tRNA-Phe free and bound to phenylalanyl-tRNA synthetase with 4-(N-2-chloroethyl-N-methylamino)benzylamine].
Reactivities toward the alkylating reagent 4-(N-2-chloroethyl-N-methylamino)benzylamine of guanosine residues in the tRNAPhe free and bound to the phenylalanyl-tRNA synthetase were determined. This highly efficient reagent modifies mainly guanosine in single-stranded as well as in helical regions of tRNAs. The reaction is sensitive to the elements of the macrostructure of tRNAs. It is found the phenylalanyl-tRNA synthetase protects the D-stem of the tRNAPhe (guanosines G10, G22, G24) from alkylation.
D000478:Alkylation; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D001483:Base Sequence; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D009150:Mustard Compounds; D009690:Nucleic Acid Conformation; D010652:Phenylalanine-tRNA Ligase / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D012343:RNA, Transfer
D009150:Mustard Compounds; C025881:4(N)-(2-chloroethyl-N-methylamino)benzylamine; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase
Alkilirovanie tRNK-Phe s fenilalanil-tRNK-sintetazoĭ Escherichia coli s pomoshch'iu 4-(N-2-khlopetil-N-metilamino)-benzilamina.