Discovery of a high affinity of Phaseolus vulgaris agglutinin (PHA) with gastrin-secreting cells.
A lectin, Phaseolus vulgaris agglutinin (PHA), isolated from the red kidney bean, was found to have a very high affinity with gastrin-secreting cells (G cells). The unique PHA-G cell interaction is confirmed by (1) G cell reaction only with PHA, not with other lectins, and (2) PHA reaction with G cells but not with other endocrine cells. The authors concluded that gastrin-secreting cells are composed of a large quantity of galactose-N-acetyl-glucose-manose-containing glycoconjugates. The significance of this glyco-conjugate in G cells is not ascertained. The use of a PHA histochemical technic may well be suitable to study the carbohydrate (or pathophysiological) changes in G cells.
D004702:Endocrine Glands / Q000166:cytology; D005753:Gastric Mucosa / Q000166:cytology* / Q000378:metabolism; D005755:Gastrins / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology* / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology; D010835:Phytohemagglutinins
D005755:Gastrins; D010835:Phytohemagglutinins