Does more than one mitochondrially synthesized protein in yeast have larger precursors?
Yeast cells undergoing derepression (the phase of mitochondriogenesis) were exposed to [14C]formate in the presence of cycloheximide, the cytosolic protein synthesis inhibitor, and of 1,10-phenanthroline, a metallo-protease inhibitor. Extensive labelling was obtained under such conditions. Incubation of these labelled products with mitochondrial lysates released small peptides (mol. wt. 500-1000). These results indicate that mitochondria probably synthesize some of the proteins in the precursor form and they are processed by a specific matrix-located protease before proper integration.
D005561:Formates / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000378:metabolism; D010618:Phenanthrolines / Q000494:pharmacology; D011498:Protein Precursors / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D005561:Formates; D010618:Phenanthrolines; D011498:Protein Precursors; D010447:Peptide Hydrolases; C025205:1,10-phenanthroline