Use of rat/hamster S-9 mixture in the Ames mutagenicity assay.
Based on the findings of Nagao et al [1978] that phenacetin is negative in the standard Ames test with Aroclor induced rat S-9 and positive with hamster S-9, the test was performed with a mixture of rat/hamster S-9. Phenacetin was mutagenic with the mixture. The activity of the mixture was compared to the rat S-9 alone with low concentrations of 2-aminoanthracene (a strong promutagen for Salmonella typhimurium TA 1535, TA 100, TA 1537, and TA 98), nitrosopiperidine (a weak promutagen), and 1,2 epoxybutane (a weak, direct-acting mutagen). Except for an increased mutagenic activation by the mixture with nitrosopiperidine the mixture was comparable to the rat S-9 alone, indicating that replacing rat S-9 with a rat/hamster S9 mixture in the standard Ames test could increase the sensitivity of the test without interfering with rat S-9 activity.
D000818:Animals; D000873:Anthracenes / Q000633:toxicity; D004852:Epoxy Compounds / Q000633:toxicity; D066298:In Vitro Techniques; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens; D009602:Nitrosamines / Q000633:toxicity; D010615:Phenacetin / Q000633:toxicity; D010880:Piperidines / Q000633:toxicity; D051381:Rats; D012486:Salmonella typhimurium / Q000235:genetics; D013347:Subcellular Fractions / Q000502:physiology*
D000873:Anthracenes; D004852:Epoxy Compounds; D009153:Mutagens; D009602:Nitrosamines; D010880:Piperidines; C027488:1,2-epoxybutane; C002743:N-nitrosopiperidine; C006593:2-anthramine; D010615:Phenacetin