Immunochemical determination of CK-MB isoenzyme in human serum. II. An enzymic approach.
A novel immunochemical technique for a specific enzymic determination of the myocardial isoenzyme of creatine kinase, CK-MB, involves determination of B-subunit activity of a specimen in which the M-subunit activity has been inhibited by specific antibodies to the M-subunit. Interfering activities from CK-BB isoenzyme, atypical forms of creatine kinase, and adenylate kinase are eliminated by using a blank tube in which all the M-subunit-containing isoenzymes have been removed by a specific immunoprecipitation step. The assay is convenient, linear, and reproducible, and results compare well with those by agarose electrophoresis.
D003402:Creatine Kinase / Q000097:blood*; D004587:Electrophoresis, Agar Gel; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000097:blood; D009203:Myocardial Infarction / Q000097:blood*; D012016:Reference Values; D013997:Time Factors
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase