Thin-layer chromatographic and high-performance liquid chromatographic separation of metabolites of the weak carcinogen, 7-methylbenz[c]acridine.
The metabolism of [14C]7-methylbenz[c]acridine, a weakly carcinogenic polycyclic aza aromatic hydrocarbon, was studied in rates in vivo, and in rat hepatocytes and hepatic microsomes. Evidence for oxidation of the methyl group and excretion of 7-hydroxymethylbenz[c]acridine and benz[c]acridine-7-carboxylic acid in rat bile was obtained by thin-layer chromatography. A reversed-phase high-performance liquid chromatographic separation of metabolites was developed and chromatographic profiles of metabolites found in vivo and formed in vitro are presented. Several synthetic potential oxidation products were used to characterise the chromatographic profiles and evidence for the in vitro formation of 7-hydroxymethylbenz[c]acridine and 5,6-dihydro-5,6-dihydroxy-7-methylbenz[c]acridine was obtained.
D000166:Acridines / Q000378:metabolism*; D000818:Animals; D001646:Bile / Q000378:metabolism; D002273:Carcinogens; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D008099:Liver / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000166:Acridines; D002273:Carcinogens; C031277:7-methylbenz(c)acridine