title: Modification of human transcortin by tetranitromethane. Evidence for the implication of a tyrosine residue in cortisol binding.
abstract: The effect of tetranitromethane on the cortisol binding activity of human transcortin has been investigated. This reagent induced a decrease of activity concomitant with nitration of tyrosine residues. An oxidation of sulphydryl groups was also observed but had no implication on cortisol binding. The nitration was specifically oriented in the site at pH6 and with low concentrations of reagent; under these conditions, a single essential tyrosine per molecule of transcortin seems implicated in cortisol binding. The absence of denaturation in modified transcortin was checked by circular dichroism spectra and polyacrylamide gel electrophoresis. Site specificity was demonstrated by full protection with cortisol against inactivation.
mesh_terms: D001665:Binding Sites / Q000187:drug effects; D006854:Hydrocortisone / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008697:Methane / Q000031:analogs & derivatives*; D013329:Structure-Activity Relationship; D013774:Tetranitromethane / Q000494:pharmacology*; D014156:Transcortin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D014443:Tyrosine; D014156:Transcortin; D013774:Tetranitromethane; D008697:Methane; D006854:Hydrocortisone