[Characterization of DNAse from rat brain].
Using gel filtration and ion exchange chromatography, a DNAse specific to a single-stranded DNA was isolated from the soluble fraction of rat brain nuclear proteins. The nuclear nuclease corresponds to alkaline DNAse obtained earlier from the intact brain. The enzyme is a Mg2+- or Mn2+-dependent exodeoxyribonuclease and hydrolyzes at the same rates the ss-DNAse from rat brain, calf thymus, E. coli and poly (dA). The major products of hydrolysis are nucleoside-5'-monophosphates. A possible role of the enzyme in reparation of neuronal DNA is discussed.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002467:Cell Nucleus / Q000201:enzymology*; D004277:DNA, Single-Stranded; D003851:Deoxyribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D051381:Rats; D013379:Substrate Specificity
D004277:DNA, Single-Stranded; D008345:Manganese; D003851:Deoxyribonucleases; D008274:Magnesium
Kharakteristika DNKazy golovnogo mozga krys.