Biochemical diagnosis of vaginitis: determination of diamines in vaginal fluid.
To assess correlations between the content of the diamines putrescine and cadaverine in vaginal fluid and the clinical manifestations of vaginitis, a rapid procedure for the determination of diamines has been developed. The procedure involves thin-layer chromatography of the dansylated vaginal washings; then the fluorescence of dansyl diamines is compared with that of dansyl alanine. For 520 college women, the result of the test for diamines was positive in 173 (88%) of 197 women with nonspecific vaginitis or vaginitis due to Trichomonas vaginalis and was negative in 291 (90%) of 323 women without nonspecific vaginitis or vaginitis due to T. vaginalis. The diamine content of vaginal fluid specimens after therapy with metronidazole was correlated with the clinical response to treatment.
D002103:Cadaverine / Q000032:analysis; D002855:Chromatography, Thin Layer; D019411:Clinical Laboratory Techniques / Q000379:methods; D003619:Dansyl Compounds / Q000032:analysis; D003959:Diamines / Q000032:analysis*; D005260:Female; D005453:Fluorescence; D006801:Humans; D008795:Metronidazole / Q000627:therapeutic use; D011700:Putrescine / Q000032:analysis; D014621:Vagina / Q000032:analysis*; D014627:Vaginitis / Q000175:diagnosis* / Q000188:drug therapy; D005680:gamma-Aminobutyric Acid / Q000032:analysis
D003619:Dansyl Compounds; D003959:Diamines; D008795:Metronidazole; D005680:gamma-Aminobutyric Acid; D002103:Cadaverine; D011700:Putrescine