Routine measurement of phosphatidylglycerol in amniotic fluid.
Phosphatidylglycerol (PG) is a constituent of lung surfactant in the mature fetus and is effective in protecting against the respiratory distress syndrome. Three methods of separating PG by thin-layer chromatography have been assessed. A two-dimensional method, although separating the major phospholipids, is not suitable for routine use. One-dimensional resolution has the advantages of speed, duplication of samples and suitability of densitometric measurement of PG. Two methods are described and a technique suitable for the rapid demonstration of amniotic fluid PG is recommended.
D000653:Amniotic Fluid / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D010715:Phosphatidylglycerols / Q000032:analysis*; D011247:Pregnancy
D010715:Phosphatidylglycerols