Diazotizable arylamine cellulose papers for the coupling and hybridization of nucleic acids.
We describe the synthesis of a family of arylamine-substituted papers which can be diazotized and coupled with nucleic acids. The synthesis is simple and uses readily obtainable starting materials. A partial characterization of the nucleic acid binding activity is reported, as well as a demonstration of the utility of the activated paper for the detection of electrophoretically separated RNA by blot transfer and hybridization.
D002482:Cellulose / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D004247:DNA; D003979:Diazonium Compounds / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D009693:Nucleic Acid Hybridization; D010209:Paper
D003979:Diazonium Compounds; D007202:Indicators and Reagents; D002482:Cellulose; D004247:DNA