[Comparative study of the proliferative activity of native and cryopreserved colony-forming units in humans and mice].
The agar culture method was used to state that colony-forming units responsible for cell structures of a hemopoietic series remained undamaged after cryopreservation (-196 degrees) in a suspension of mouse and human donor bone marrow. Cultivation of cryopreserved bone marrow revealed the inhibition of proliferative activity of cells growing in agar as compared with native ones. The inhibition of DNA synthesis was the basis of this process.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D003114:Colony-Forming Units Assay; D003451:Cryoprotective Agents / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D005615:Freezing; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011092:Polyethylene Glycols / Q000494:pharmacology; D013997:Time Factors
D003451:Cryoprotective Agents; D011092:Polyethylene Glycols; D004247:DNA
Sravnitel'noe izuchenie proliferativnoĭ aktivnosti nativnykh i kriokonservirovannykh kolonieobrazuyiushchikh edinits cheloveka i mysheĭ.