[Problems in using radioactively labeled bacteria in animal experiments. 4. Distribution of activities in the mouse following intratracheal administration of labeled Pasteurella multocida].
Intratracheal administration of 3H-labelled, 14C-labelled, 59Fe-labelled or 125J-Labelled Pasteurella multicida germs to mice resulted in more or less differentiated, nuclide-dependent, distributions of radioactivity in blood, spleen, liver, lung, kidney, and gastro-intestinal tract. All distributions were comparable to those following subcutaneous application. Elimination of antigen from lungs and other organs could be characterised by an e-function, once a certain level of distribution had been reached. Some of the antigen was persistent in the lung not less than 14 days. Extremely high activity concentration and persistence was recordable, following the use of 59Fe complete antigen. Phagocytosis of Pasteurella multicida germs through alveolar macrophages of the lung was secured by autoradiography. Most of the antigen seemed to be discharged from the lungs through the digestive tract. Antigen distributions recorded from immunised and non-immunised mice seemed to suggest that the fate of antigen applied was affected by the kind of immunisation. No difference in antigen distribution between non-immunised and subcutaneously immunised animals were recordable, following intratracheal antigen application, but is was clearly recordable, following intratracheal immunisation. Elimination of antigen from the lungs of intratracheally immunised animals was found to occur faster than it did from non-immunised animals.
D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000032:analysis*; D002250:Carbon Radioisotopes; D007267:Injections; D007279:Injections, Subcutaneous; D007457:Iodine Radioisotopes; D007504:Iron Radioisotopes; D007553:Isotope Labeling; D051379:Mice; D010325:Pasteurella / Q000276:immunology*; D014018:Tissue Distribution; D014132:Trachea; D014316:Tritium
D000942:Antigens, Bacterial; D002250:Carbon Radioisotopes; D007457:Iodine Radioisotopes; D007504:Iron Radioisotopes; D014316:Tritium
Probleme des Einsatzes radioaktiv markierter Bakterien bei Tierversuchen. 4. Mitteilung: Aktivitätsverteilung im Müseorganismus nach intratrachealer Verabreichung von markeierten Pasteurella-multocida-Keimen.