title: A method for quantitative extraction of enzymes and metabolites from tissue samples in the milligram range.
abstract: A method is described for quantitative extraction of soluble enzyme activities from tissue samples in the milligram range. Mechanical disintegration of the frozen tissue is performed in a micromortar from which the powdered tissue can be quantitatively transferred for extraction in small volumes within a microcentrifuge tube.
mesh_terms: D000818:Animals; D004798:Enzymes / Q000302:isolation & purification*; D008722:Methods; D051379:Mice; D009132:Muscles / Q000032:analysis* / Q000201:enzymology
chemical_list: D004798:Enzymes