Determination of total bile acids in serum. A comparison of a radioimmunoassay with an enzymatic-fluorimetric method.
A solid phase radioimmunoassay kit method for total conjugated bile acids has been compared to an enzymatic fluorimetric method for total serum bile acids. The methods were compared with respect to: precision, cross-reactivity (molar equivalence) of different bile salts, recovery of different bile salts from serum, the reference range for a healthy population, linearity, coefficient of correlation, diagnostic effectiveness, cost and ease of assay. Both assays seemed equally capable of predicting the presence or absence of liver disease. Radioimmunoassay had little advantage over the enzymatic-fluorimetric method. Its relative ease was far outweighed by its greater cost and poorer analytical performance.
D000328:Adult; D001647:Bile Acids and Salts / Q000097:blood* / Q000276:immunology; D003365:Costs and Cost Analysis; D003429:Cross Reactions; D005260:Female; D006801:Humans; D008297:Male; D011863:Radioimmunoassay / Q000379:methods; D011933:Reagent Kits, Diagnostic; D013050:Spectrometry, Fluorescence / Q000379:methods
D001647:Bile Acids and Salts; D011933:Reagent Kits, Diagnostic