Structure of an amniotic fluid component, 7-(4,5-cis-dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanine (queuine), a substrate for tRNA: guanine transglycosylase.
A compound present in bovine amniotic fluid, and which is a substrate for tRNA:guanine transglycosylase, has been structurally characterized as queuine, a hypermodified base which occurs in transfer RNA. The structure assignment is based on mass spectrometry, proton nuclear magnetic resonance, and ultraviolet absorbance data.
D000653:Amniotic Fluid / Q000032:analysis*; D000818:Animals; D001681:Biological Assay; D002417:Cattle; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010430:Pentosyltransferases; D011247:Pregnancy; D013056:Spectrophotometry, Ultraviolet; D014166:Transferases / Q000378:metabolism*
D006147:Guanine; C019549:queuine; D014166:Transferases; D010430:Pentosyltransferases; C020837:queuine tRNA-ribosyltransferase