title: A rapid method for identification of constitutive heterochromatin of secondary constriction regions of human chromosomes 1, 9, and 16.
abstract: The secondary constriction regions (h) of chromosomes 1, 9, and 16 stained deep red by RBA (R-bands by BrdU using Giemsa)--a useful tool for demonstrating the constitutive heterochromatic heteromorphisms in these regions. The unusual staining pattern of bands p 12 to q11 of chromosome 3, which is late replicating, also is reported. The extended deep red region does not correspond to C-banding on chromosome 3. This technique does not require sequential banding by QFQ and CBG for detecting the h-region heteromorphisms.
mesh_terms: D000165:Acridine Orange; D001973:Bromodeoxyuridine; D002478:Cells, Cultured; D002871:Chromosome Banding; D002900:Chromosomes, Human, 1-3 / Q000648:ultrastructure*; D002902:Chromosomes, Human, 16-18 / Q000648:ultrastructure*; D002906:Chromosomes, Human, 6-12 and X / Q000648:ultrastructure*; D006570:Heterochromatin / Q000648:ultrastructure*; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D011110:Polymorphism, Genetic
chemical_list: D006570:Heterochromatin; D000165:Acridine Orange; D001973:Bromodeoxyuridine