Liquid diffraction analysis of sarcoplasmic reticulum. II. Solvent electron contrast variation.
Intensities of x-ray scattering from rabbit muscle sarcoplasmic reticulum membrane have been measured over the range of s = 0.05-0.25, at solvent densities varying between p0 = 0.335 and 0.389 electrons/A3. Analysis of the results shows agreement with the elements of structure deduced earlier by a different technique, based on the variation of the lipid-protein concentration ratio. In addition, the present work extends the analysis to allow isolation of the lipid contribution to the total scattering, from which a profile of the lipid electron density normal to the membrane face is evaluated. The scattering arising from electron correlations within the plane of the bilayer has also been identified.
D000818:Animals; D008433:Mathematics; D008563:Membrane Lipids / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008722:Methods; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000648:ultrastructure*; D012542:Scattering, Radiation; D014965:X-Rays
D008563:Membrane Lipids; D008565:Membrane Proteins