Hemoglobins, XLV. The amino acid sequence of pheasant (Phasianus colchicus colchicus) hemoglobins.
Pheasant hemoglobin is heterogenous and contains two components: a major one called HbA (70% of total amount) and a minor one called HbD (25% of total amount). HbA contains alpha A-chain, while HbD contains alpha D-chain. The amino acid sequences of both hemoglobins are given. The sequences were determined by automatic Edman degradation of tryptic peptides and peptides obtained by specific chemical cleavages. Between alpha A- and alpha D-chains a large difference of 57 amino acids was found. The primary structures of beta-chains from both hemoglobins are identical. The alpha A-chains contain no tryptophan, only one methionine and two cysteines. The alpha D-chains contain one tryptophan, four methionines and one cysteine. By homologous comparison with greylag goose hemoglobin the alpha A-chains differ by 19 exchanges, beta-chains by 7 exchanges. The alpha D-chains differ by 10 exchanges in comparison to alpha D-chains from chicken hemoglobin D. In the alpha A-chains valine was found to position alpha 63 (E12) as was the case for alpha-chains of barheaded goose. In the alpha D-chains however leucine was found in this position.
D000595:Amino Acid Sequence; D000818:Animals; D001717:Birds / Q000097:blood*; D005777:Geese / Q000097:blood; D006441:Hemoglobin A; D006454:Hemoglobins / Q000378:metabolism; D006455:Hemoglobins, Abnormal; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D010108:Oxyhemoglobins / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis; D013045:Species Specificity; D014357:Trypsin
D006454:Hemoglobins; D006455:Hemoglobins, Abnormal; D046911:Macromolecular Substances; D010108:Oxyhemoglobins; D010446:Peptide Fragments; C032001:hemoglobin D; D006441:Hemoglobin A; D014357:Trypsin