Dowex-1-induced reactions of reduced nicotinamide adenine dinucleotide (NADH).
Dowex 1-formate has been found to cause both anomerization and oxidation of NADH, and when NADH is chromatographed on a column of this resin, the major products observed are NAD+ and alpha-NAD+. Competing with the oxidation reaction is the conversion of NADH and alpha-NADH to unstable acid-modification products that subsequently break down during chromatography to give ADP-ribose and a variety of neutral and cationic degradation products. The effects of Dowex 1-formate on NADH differ from those of acid as oxidation is minimal when NADH is incubated in acid solution, although anomerization, acid-modification, and degradation to ADP-ribose and other products readily occur. The neural and cationic acid-degradation products that are formed from acid-modified NADH have been resolved by chromatography into 12 compounds, 6 of which react with 3-methyl-2-benzothiazolinone hydrazone and thus are identified as carbonyls. These substances gradually disappear from acid solution over a period of days and are replaced by polymeric pigments.
D000837:Anion Exchange Resins; D005561:Formates; D006863:Hydrogen-Ion Concentration; D007475:Ion Exchange Resins; D007700:Kinetics; D009243:NAD / Q000302:isolation & purification; D010084:Oxidation-Reduction; D012117:Resins, Synthetic; D013056:Spectrophotometry, Ultraviolet
D000837:Anion Exchange Resins; D005561:Formates; D007475:Ion Exchange Resins; D012117:Resins, Synthetic; D009243:NAD; C030544:formic acid; C027972:Dowex