An improved technique for the isolation of lymphocytes from small volumes of peripheral mouse blood.
Centrifugation of mouse blood on density gradients of polyvinylpyrrolidone-coated colloidal silica (Percoll) resulted in the simultaneous separation of platelets, lymphocytes, granulocytes and erythrocytes. By this method, we have been successful in isolating a fraction of highly purified and viable lymphocytes from small volumes of peripheral mouse blood with good recovery.
D000818:Animals; D001792:Blood Platelets; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002499:Centrifugation, Density Gradient; D004912:Erythrocytes; D005260:Female; D006098:Granulocytes; D008213:Lymphocyte Activation; D008214:Lymphocytes; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010835:Phytohemagglutinins / Q000494:pharmacology
D010835:Phytohemagglutinins