[Clonogenic cultures of human tumours. Experience and prospects in cancer chemotherapy (author's transl)].
Malignant cells grow selectively in semi-solid media. Cultures of single cell suspensions in these media result in the formation of colonies which are believed to represent clones of stem cells. Cells freshly sampled in cancer patients are assayed in such a system to quantitate the in vitro effect of various anticancer agents vs untreated controls. Partially retrospective comparisons between in vitro and in vivo data are very encouraging and suggest that observations in this assay correlate very favorably with clinical findings. This clonogenic assay should provide an experimental model of special relevance to clinical cancer chemotherapy and should greatly help define optimal treatments in individual patients. Although many technical problems remain to be settled, prospective studies are ongoing to determine to what extent the assay is actually predictive of the response to chemotherapy in cancer patients.
D000970:Antineoplastic Agents / Q000627:therapeutic use*; D002999:Clone Cells / Q000166:cytology*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006801:Humans; D009374:Neoplasms, Experimental / Q000188:drug therapy
D000970:Antineoplastic Agents
Cultures clonogéniques de tumeurs humaines. Expérience et perspectives pour la chimiothérapie du cancer.