title: Chloroplast biogenesis. Detection of monovinyl magnesium-protoporphyrin monoester and other monovinyl magnesium-porphyrins in higher plants.
abstract: It is shown that the magnesium-protoporphyrin-6-ester pool of plants is made up of monovinyl and divinyl components which were separated by chromatography on thin layers of polyethylene. At 77K, in ether, monovinyl-Mg-protoporphyrin-6-ester exhibited fluorescence emission and excitation maxima at 589 and 417 nm, respectively. The divinyl-Mg-protoporphyrin-6-ester component exhibited red-shifted emission and excitation maxima at 591 and 424 nm respectively. Demetallation of the monovinyl and divinyl Mg-protoporphyrin ester components converted them into monovinyl and divinyl-protoporphyrin IX free bases. Catalytic Mg-mesoporphyrin ester and the demetallated free bases into mesoporphyrin ester, Monovinyl-Mg-porphyrin chemical species were also detected in the Mg-protoporphyrin IX and the Mg-protoporphyrin IX diester pools of plants. It is proposed that the Mg-protoporphyrin IX, Mg-protoporphyrin IX-6-ester, and Mg-protoporphyrin IX-diester pools of plants are heterogenous and are made up of monovinyl and divinyl chemical species.
mesh_terms: D002736:Chloroplasts / Q000378:metabolism*; D003624:Darkness; D005615:Freezing; D008274:Magnesium / Q000032:analysis*; D010944:Plants / Q000032:analysis*; D011166:Porphyrins / Q000032:analysis*; D011524:Protoporphyrins / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013329:Structure-Activity Relationship; D015032:Zinc / Q000032:analysis
chemical_list: D011166:Porphyrins; D011524:Protoporphyrins; C033434:protoporphyrin IX monomethyl ester; D008274:Magnesium; D015032:Zinc