High-performance liquid chromatography of benzophenone derivatives for the determination of benzodiazepines in clinical emergencies.
A screening procedure for fifteen 1,4-benzodiazepines in biological material (urine), suitable for clinical toxicology, is described. The benzodiazepines and/or their metabolites are hydrolysed to their benzophenones; after alkalinization, the benzophenones are extracted with chloroform. The extract is evaporated to dryness and the dried residue is dissolved in methanol and analysed by high-performance liquid chromatography on a reversed-phase column at 254 nm with methanol-water (1:1) as eluent.
D001569:Benzodiazepines / Q000378:metabolism / Q000652:urine*; D001577:Benzophenones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D014116:Toxicology
D001577:Benzophenones; D001569:Benzodiazepines