Fluorescein binding to normal human serum proteins demonstrated by equilibrium dialysis.
The binding of fluorescein to normal human serum proteins in a physiologic solvent at 37 degree C was measured by equilibrium dialysis. Human serum contained 3.28 X 10(-3)M concentration fluorescein-binding sites, with an average association constant at 37 degree C of 0.54 X 10(4)M(-1). The percentage of total fluorescein bound by human serum proteins ranged from 83% to 53% when the total fluorescein concentration ranged from 6.9 X 10(-5) to 6.2 X 10(-3)M.
D001665:Binding Sites; D001798:Blood Proteins / Q000378:metabolism*; D003956:Dialysis; D005452:Fluoresceins / Q000378:metabolism*; D006801:Humans; D008297:Male; D011485:Protein Binding
D001798:Blood Proteins; D005452:Fluoresceins