Improved transport of horseradish peroxidase after injection with a non-ionic detergent (Nonidet P-40) into mouse cortex and observations on the relationship between spread at the injection site and amount of transported label.
Addition of the non-ionic detergent Nonidet P-40 (NP-40) to horseradish peroxidase (HRP) injected in small quantities into barrelfields of mouse somatosensory cortex results in a significant increase of labeled neurons in the contralateral barrelfield cortex as compared to normal HRP. Comparisons with lysolethicin as an additive to HRP show that with NP-40 neurons are labeled more reliably and spread of label is less extensive at the injection site. Using NP-40, the region of dense label spread at the injection site as revealed by the diaminobenzidine/cobalt procedure coincides rather precisely with the contralateral cortical region containing labeled neurons as visualized by tetramethylbenzidine.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects*; D005260:Female; D006735:Horseradish Peroxidase / Q000378:metabolism*; D007267:Injections; D051379:Mice / Q000502:physiology*; D008813:Mice, Inbred ICR; D009474:Neurons / Q000378:metabolism; D017830:Octoxynol; D010544:Peroxidases / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000008:administration & dosage* / Q000494:pharmacology; D013003:Somatosensory Cortex / Q000378:metabolism*
D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D006735:Horseradish Peroxidase; D010544:Peroxidases