title: Problems in the measurement of putative serum immune complexes by the method of Beaumont.
abstract: A methodological investigation of the procedure used by Beaumont et al for measuring a "serum immune complex" precipitated by 25% ammonium sulfate and alleged to contain an ethynyl-estradiol binding immunoglobulin has found major problems with reproducibility and with the correlation of total protein as measured by the Lowry method and the IgG content as determined by a specific nephelometric procedure.
mesh_terms: D000936:Antigen-Antibody Complex / Q000032:analysis*; D011232:Chemical Precipitation; D003276:Contraceptives, Oral / Q000009:adverse effects / Q000276:immunology; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D007158:Immunologic Techniques; D013924:Thrombophlebitis / Q000139:chemically induced / Q000276:immunology
chemical_list: D000936:Antigen-Antibody Complex; D003276:Contraceptives, Oral