A simple technique for silicone rubber perfusion of the microcirculation of the mouse kidney.
A technique was described in which mouse kidneys were perfused with a silicone rubber compound. The technique involved cannulation of the left ventricle of the heart and subsequent perfusion of the entire systemic circulation of the mouse. Constant pressure was maintained to prevent distortion artifacts in the resultant renal microvascular cast. The method is adaptable to perfusion of other organs or animals.
D000818:Animals; D007668:Kidney / Q000098:blood supply*; D051379:Mice; D008833:Microcirculation; D010477:Perfusion / Q000379:methods*; D011312:Pressure; D012826:Silicone Elastomers
D012826:Silicone Elastomers