Biochemical changes induced in human plasma by its lipoprotein lipase.
1. Incubation of human citrated plasma with a concentrate of lipoprotein lipase (separated from the same plasma pool), for 6 hr at 37 degree C and pH 7.0, caused a marked decrease in the concentrations of calcium, triglycerides, total phosphorus, lipid phosphorus and total fatty acids. 2. There was a concomitant increase in the concentrations of non-esterified fatty acids and inorganic phosphorus which were presumably formed by enzymatic hydrolysis of triglycerides and lipid phosphorus. 3. These changes in human plasma, induced by its lipoprotein lipase, were quantitatively comparable to the same changes which have been previously reported in liquid plasma between the 1st and 2nd months of storage at room temperature.
D002118:Calcium / Q000097:blood; D005227:Fatty Acids / Q000097:blood; D006801:Humans; D008071:Lipoprotein Lipase / Q000097:blood*; D010743:Phospholipids / Q000097:blood; D010758:Phosphorus / Q000097:blood; D014280:Triglycerides / Q000097:blood
D005227:Fatty Acids; D010743:Phospholipids; D014280:Triglycerides; D010758:Phosphorus; D008071:Lipoprotein Lipase; D002118:Calcium