Measurement in human blood of fibrinogen/fibrin fragments containing the B beta 15-42 sequence.
This paper describes a radioimmunoassay for the B beta 15-42 peptide derived from human fibrinogen or fibrin. Iodinated B beta 15-42 is bound by specific antiserum and binding can be completely inhibited by excess of the non-iodinated B beta 15-42, B beta 1-42 or B beta 1-118. These peptides cannot be distinguished in this assay. Furthermore, fibrinogen can also completely inhibit binding of iodinated B beta 15-42 peptide. Due to the cross-reaction with fibrinogen, clinical blood samples require a processing step prior to their use in this radioimmunoassay. Ethanol precipitation allows for fibrinogen removal and a near quantitative recovery of both added B beta 15-42 peptide as well as of the endogenous blood peptide(s) containing the B beta 15-42 sequence. The mean level of B beta 15-42 immunoreactive material in normal individuals was found to be 0.41 pmol/ml while that in one group of patients was 20-40 times this value.
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000032:analysis; D001667:Binding, Competitive; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007676:Kidney Failure, Chronic / Q000097:blood; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments; D011817:Rabbits; D011863:Radioimmunoassay
D000941:Antigens; D005338:Fibrin Fibrinogen Degradation Products; D007106:Immune Sera; D010446:Peptide Fragments; C033786:fibrinogen Bbeta (15-42)