Rapid determination of ammonia in whole blood and plasma using flow injection analysis.
A flow injection method for the determination of ammonia in whole blood and plasma is described. The method utilizes diffusion of ammonia into a stream of a pH-sensitive indicator, which is monitored by a photometer. A volume of 90 microliter is needed and the result is obtained within 1 min. Ammonia concentrations increase during storage due to deamination. It is proposed to use plasma samples, which could be stored frozen at - 20 degrees C without significant change.
D000641:Ammonia / Q000097:blood*; D004912:Erythrocytes / Q000032:analysis; D006801:Humans; D008722:Methods; D010783:Photometry; D013048:Specimen Handling; D013696:Temperature; D013997:Time Factors
D000641:Ammonia