Ultrathinfilm immunoelectrophoresis.
Immunoelectrophoresis (Laurell-rocket) in ultrathinfilm gels has been applied to the determination of human serum albumin in a model study. Agarose gels of 0.24 mm thickness were used and optimum electrophoretic conditions were determined. Precision was assessed and comparison with conventional immunoelectrophoresis and a chemical method was made. Though precision was poor (CV 4-6%) improvements in dispensing small volumes (0.2 microliter) should change this. Good correlation between methods (correlation coefficient 0.89-0.97) was obtained. The advantages of the technique are speed of analysis and low reagent costs.
D001797:Blood Protein Electrophoresis / Q000379:methods; D005782:Gels; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D012709:Serum Albumin / Q000032:analysis*
D005782:Gels; D012709:Serum Albumin