Dye-binding method for rapid colorimetric determination of the cholestasis characterizing lipoprotein-X.
Serum lipoproteins were stained by the addition of Sudan Black B solution. The normal lipoproteins and the excess of Sudan Black were removed with heparin and zinc acetate solution. The abnormal lipoprotein-X remained in the supernatant, the blue colour of which was measured. The empirically obtained constant was used for calculation, thus avoiding standardization.
D001391:Azo Compounds; D002779:Cholestasis / Q000097:blood*; D003124:Colorimetry; D004396:Coloring Agents; D006801:Humans; D008073:Lipoprotein-X / Q000097:blood*; D009281:Naphthalenes
D001391:Azo Compounds; D004396:Coloring Agents; D008073:Lipoprotein-X; D009281:Naphthalenes; C016118:Sudan Black B