Purification of cytoplasmic aldehyde dehydrogenase by covalent chromatography on reduced thiopropyl-Sepharose 6B.
Disulfiram-modified cytoplasmic aldehyde dehydrogenase binds covalently to the thiol groups of reduced thiopropyl-Sepharose 6B under conditions in which mitochondrial aldehyde dehydrogenase does not bind. After washing the resin, the uncontaminated form of the enzyme is eluted by dithiothreitol solution.
D000445:Aldehyde Oxidoreductases / Q000302:isolation & purification*; D000818:Animals; D002847:Chromatography, Agarose; D003593:Cytoplasm / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D012756:Sheep
D000445:Aldehyde Oxidoreductases