Radioimmunoassay and chemical ionization/mass spectrometry compared for plasma cortisol determination.
We describe a method for determination of cortisol in plasma and urine, based on chemical ionization/mass spectrometry with deuterium-labeled cortisol as the internal standard. The within-run precision (CV) was 2.5-5.7%, the between-run precision 4.6%. Results by this method were compared with those by a radioimmunological method (RIANEN Cortisol, New England Nuclear) for 395 plasma samples. The latter method gave significantly higher (approx. 25%) cortisol values.
D000287:Administration, Topical; D000328:Adult; D008401:Gas Chromatography-Mass Spectrometry; D005938:Glucocorticoids / Q000008:administration & dosage; D006801:Humans; D006854:Hydrocortisone / Q000097:blood* / Q000652:urine; D011863:Radioimmunoassay; D012016:Reference Values
D005938:Glucocorticoids; D006854:Hydrocortisone