[Reconstruction of the organotypic structure of embryonic lung tissue from a suspension of dissociated cells].
The formation and development of organotypic structure of the embryonic lung tissue was studied upon the cultivation of a suspension of dissociated cells of the lungs of 8-11 days old chick embryos as a hanging drop. The successive developmental stages of organotypic structures are discussed: 1) passive sedimentation of dissociated cells on the "bottom" of hanging drop; 2) redistribution of sedimented cells on the tension surface of the drop with the formation of multiple cell aggregations (centers of aggregation) connected by monolayer mesenchyme constrictions; 3) formation of a primary aggregate as a flat celled structure; 4) formation of a space organotypic aggregate with the structure characteristic for the initial lung tissue.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D008168:Lung / Q000196:embryology*; D009024:Morphogenesis; D013535:Suspensions; D013997:Time Factors
D013535:Suspensions
Rekonstruktsiia organotipicheskoistruktury émbrional'noĭ legochnoĭ tkani iz suspenzii dissotsiirovannykh kletok.