Retention of carcinoembryonic antigen on Sephadex G-50 and acrylamide gel buffer-exchange columns.
We compared the degree of retention of carcinoembryonic antigen on two types of buffer-exchange columns, Sephadex G-50 and acrylamide gel. Both types retained trace amounts of the antigen applied in the form of a perchloric acid extract, as measured by its release into plasma samples subsequently processed through the same columns, Cross contamination, resulting in values falsely high by greater than 1 microgram/L, was observed when columns were re-used after processing plasma samples with carcinoembryonic antigen concentrations greater than 700 micrograms/L.
D001774:Blood Chemical Analysis; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D002850:Chromatography, Gel; D005189:False Positive Reactions; D006801:Humans; D006863:Hydrogen-Ion Concentration
D002272:Carcinoembryonic Antigen