Effects of 8-(N, N-diethylamino)ocytl 3, 4, 5-trimethoxybenzoate HCl (TMB-8) on washed blood platelets with special reference to a specific loss of serotonin.
8-(N,N-diethylamino)octyl 3,4,5-trimethoxybenzoate HCl (TMB-8) has been used to indicate involvement of Ca++ in platelet activities, but possible other effects have not been investigated. This study investigates TMB-8-induced leakage of cytoplasmic content and specific loss of one granule constituent, serotonin from washed platelets. TMB-8 concentrations above 0.5 mM block secretion initially and induce leakage after short time incubation. TMB-8 (0.15 mM) enhances a slow release of serotonin, but inhibits a slow release of low affinity platelet factor 4 antigen which take place when washed platelets are incubated at pH 7.4 and 37 degree C. These effects should be taken into consideration when TMB-8 is used as a calcium antagonist in platelets.
D000227:Adenine Nucleotides / Q000097:blood; D000941:Antigens; D001779:Blood Coagulation Factors / Q000276:immunology; D001792:Blood Platelets / Q000187:drug effects*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000378:metabolism; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D007099:Imipramine / Q000096:biosynthesis; D010455:Peptides; D012701:Serotonin / Q000097:blood*; D013917:Thrombin / Q000494:pharmacology
D000227:Adenine Nucleotides; D000941:Antigens; D001779:Blood Coagulation Factors; D002121:Calcium Channel Blockers; D010455:Peptides; D012701:Serotonin; C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D005707:Gallic Acid; C009862:connective tissue-activating peptide; C018329:low affinity platelet factor 4; D013917:Thrombin; D007099:Imipramine