The uptake of [3H]gamma-aminobutyric acid by isolated glial (Müller) cells from the mouse retina.
We have examined the uptake of [3H]GABA by isolated glial (Müller) cells using light microscope autoradiography. The aim of these studies was to determine whether Müller cells dissociated from papain-treated mouse retina have the capacity to accumulate exogenous GABA and to further characterize the uptake process. Our results show that isolated Müller cells retain the capacity to accumulate exogenous [3H]GABA and this uptake is inhibited by excess GABA, DABA and beta-alanine. This communication also describes a new method for performing light microscope autoradiography.
D000409:Alanine / Q000494:pharmacology; D000613:Aminobutyrates / Q000494:pharmacology; D000818:Animals; D001345:Autoradiography / Q000379:methods*; D046508:Culture Techniques; D005260:Female; D051379:Mice; D009474:Neurons / Q000378:metabolism; D012160:Retina / Q000166:cytology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D000613:Aminobutyrates; C005959:2,4-diaminobutyric acid; D005680:gamma-Aminobutyric Acid; D000409:Alanine