The preparation of thyroid microsomal antigen for use in the indirect micro-ELISA method for the detection of anti-thyroid microsomal autoantibody.
Thyroid microsomes for use in assay systems for anti-thyroid-microsomal antibodies are normally prepared by ultracentrifugation. By means of gel filtration studies we show that preparations made in this way are invariably contaminated with thyroglobulin. A further purification by gel filtration is described which enables the preparation of thyroid microsomes of sufficient purity for use in a micro-ELISA method for the measurement of anti-thyroid-microsomal antibodies. Microsomes prepared in this way would also be suitable for haemagglutination and radioassays, which are also affected by thyroglobulin contamination.
D000941:Antigens / Q000302:isolation & purification; D001323:Autoantibodies / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006386:Hemagglutination Tests; D006801:Humans; D066298:In Vitro Techniques; D008861:Microsomes / Q000276:immunology*; D011863:Radioimmunoassay / Q000379:methods; D013954:Thyroglobulin / Q000302:isolation & purification; D013961:Thyroid Gland / Q000276:immunology*; D014461:Ultracentrifugation
D000941:Antigens; D001323:Autoantibodies; D013954:Thyroglobulin