Quantitative histochemical resolution of the oxidation-reduction and phosphate potentials within the simple hepatic acinus.
Glycerin-3-P, commonly known as alpha-glycerophosphate and dihydroxyacetone phosphate (DHAP) were measured in well defined microscopic samples of the simple liver acinus allowing a comparison of the glycerin-3-P/dihydroxyacetone-P-ratios of Zones 1 and 3 as a measure of the free NAD+/NADH ratio. The ATP/ADP X Pi quotients were determined in these same microscopic areas of the liver acinus as a measure of the phosphate potential. Brief ischemia was used to disturb the system. The results indicate that the oxidation-reduction and phosphate potentials are uniform throughout the entire liver lobule.
D000818:Animals; D004099:Dihydroxyacetone Phosphate / Q000378:metabolism*; D005994:Glycerophosphates / Q000378:metabolism*; D007511:Ischemia / Q000378:metabolism; D008099:Liver / Q000098:blood supply / Q000166:cytology / Q000378:metabolism*; D008297:Male; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains; D014306:Trioses / Q000378:metabolism*
D005994:Glycerophosphates; D014306:Trioses; D009243:NAD; D009249:NADP; D004099:Dihydroxyacetone Phosphate; C029620:alpha-glycerophosphoric acid