title: Synthesis of repressible acid phosphatase in Saccharomyces cerevisiae under conditions of enzyme instability.
abstract: The synthesis of repressible acid phosphatase in Saccharomyces cerevisiae was examined under conditions of blocked derepression as described by Toh-e et al. (Mol. Gen. Genet. 162:139-149, 1978). Based on a genetic and biochemical analysis of the phenomenon these authors proposed a new regulatory model for acid phosphatase expression involving a simultaneous interaction of regulatory factors in the control of structural gene transcription. We demonstrate here that under growth conditions that fail to produce acid phosphatase the enzyme is readily inactivated. Furthermore, we demonstrate under these conditions the production of acid phosphatase mRNA which is active both in vitro and in vivo in the synthesis of enzyme. This eliminates any step prior to translation of acid phosphatase polypeptide as an explanation for the phenomenon. We interpret our results for the block in appearance of acid phosphatase as a result of both deaccelerated growth and cellular biosynthesis during derepression, accompanied by an enhanced instability of the enzyme.
mesh_terms: D000135:Acid Phosphatase / Q000096:biosynthesis*; D000645:Ammonium Sulfate / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D004794:Enzyme Repression / Q000187:drug effects; D005656:Fungal Proteins / Q000096:biosynthesis; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D006863:Hydrogen-Ion Concentration; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D012333:RNA, Messenger; D000135:Acid Phosphatase; D000645:Ammonium Sulfate