[Respiration activity of frozen-thawed lymphoid cells].
The respiration and oxidative phosphorylation rate of lymphocytes were studied after their cryopreservation. The oxygen uptake of cells frozen in the freezing medium containing 20% serum and 10% PEO-400 or DMSO is higher than that of the native lymphocytes. 2,4-DNP has not demonstrated any stimulating effect on the frozen-thawed cell respiration. Succinate has significantly stimulated the respiration of cells frozen with PEO-400 and produces a slight stimulating effect on the respiration of cells frozen with DMSO. It is concluded that the mitochondria and plasma membranes of cryopreserved lymphocytes undergo considerable changes.
D000818:Animals; D005615:Freezing; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D011309:Preservation, Biological; D051381:Rats; D012997:Solvents
D012997:Solvents
Dykhatel'naia aktivnost' dekonservirovannykh limfoidnykh kletok.