Identification of 7-carboxymethylguanine in DNA from pancreatic acinar cells exposed to azaserine.
Studies were undertaken to determine the identity of an azaserine:DNA adduct. The most probable adduct, 7-carboxymethylguanine, was synthesized. DNA isolated from pancreatic acinar cells treated in culture with [14C]azaserine was hydrolyzed under neutral conditions to liberate N-alkylated purines. The neutral hydrolysate was subjected to high-performance liquid chromatography along with the synthetic standard. One of the radioactive peaks from the treated DNA was found to cochromatograph with 7-carboxymethylguanine in three systems: reverse phase; anion exchange; and ion pair reverse phase. These results suggest that azaserine metabolism in acinar cells results in carboxymethylation of DNA, supporting previously proposed models of azaserine degradation.
D000478:Alkylation; D000818:Animals; D001377:Azaserine / Q000378:metabolism*; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D006147:Guanine / Q000031:analogs & derivatives* / Q000032:analysis; D010179:Pancreas / Q000378:metabolism*; D051381:Rats
D006147:Guanine; C034100:7-carboxymethylguanine; D001377:Azaserine; D004247:DNA