title: [Chronic tick-bone encephalitis virus infection in continuous human lymphocyte lines studied by molecular hybridization].
abstract: Chronic infection with tick-borne encephalitis virus (Sophyin strain) has been established in continuous lines of human lymphocytes (of T- and B-origin). In 3 lines under study, the cultured virus at the level of the 15th passage had a titre of 7.22-8.02 lg LD50ml, the virus-specific antigen was determined in the cytoplasm of 80% of T-lymphocytes (line 1387). The method of RNA-DNA hybridization demonstrated the presence of virus-specific sequences in DNA preparations from infected cells in amounts not exceeding 1 copy of virus genome per 10-25 cells.
mesh_terms: D002460:Cell Line; D002908:Chronic Disease; D004247:DNA / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D004669:Encephalitis Viruses, Tick-Borne / Q000032:analysis*; D004675:Encephalitis, Tick-Borne / Q000382:microbiology*; D005814:Genes, Viral; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis*; D013045:Species Specificity; D014776:Virus Cultivation
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D004247:DNA
vernacular_title: Izuchenie khronicheskoĭ infektsii virusa kleschevogo éntsefalita v perevivaemykh liniiakh limfotsitov cheloveka metodom molekuliarnoĭ gibridizatsii.