The effect of phentolamine on synaptosomal phosphatidylinositol in experimental galactose toxicity.
Abnormal myo-[2-3H]inositol incorporation into phosphatidylinositol has been found in phentolamine-treated synaptosomes that were isolated from the cerebral hemispheres of galactose toxic rats and incubated with [33P]Pi and myo-[2-3H] inositol. In galactose toxic rats phentolamine-stimulated myo-[2-3H]inositol labeling of phosphatidylinositol was 70% greater than in normal animals. This enhanced labeling of synaptosomal phosphatidylinositol in galactose toxic rats during stimulation with phentolamine is in marked contrast to the depressed myo-inositol labeling of phosphatidylinositol reported with acetylcholine stimulation.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D005690:Galactose / Q000633:toxicity*; D007294:Inositol / Q000378:metabolism; D010646:Phentolamine / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000096:biosynthesis*; D051381:Rats; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*
D010716:Phosphatidylinositols; D007294:Inositol; D005690:Galactose; D010646:Phentolamine