Rapid processing of biopsy specimens for examination by electron microscopy.
A rapid method for embedding biopsies and other small specimens for examination by electron microscopy is described. This method is based on an embedding medium consisting of vinyl cyclohexane dioxide and cyanoacrylate which infiltrates into small specimens and polymerizes to form a cured specimen block within 15 minutes. Fixation, dehydration, infiltration and curing of specimens are all accomplished with 1 hour. The embedded specimens are relatively easy to section, stain by commonly employed electron-dense stains and are very stable under the electron beam. All specimens studied including blood, bacteria, and biopsy samples retained the salient ultrastructural features commonly preserved during lengthy methods of embedding. This method is designed for diagnostic laboratories that require rapid results.
D000818:Animals; D001706:Biopsy; D003890:Desiccation; D005404:Fixatives; D008297:Male; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*; D013997:Time Factors
D005404:Fixatives