Experimental cerebral ischemia produced by extracranial vascular injury: protection with indomethacin and prostacyclin.
To determine whether the production of brain ischemia is modified by antiplatelet agents administered at the time of extracranial endothelial injury, we modified a four-vessel occlusion rat model so that an electrogenic platelet thrombus in one carotid artery produced cerebral ischemia. DC current was passed through an anode around the left carotid artery of 11 rats with preoccluded vertebral and contralateral carotid arteries. Five of six untreated rats became unresponsive because of carotid occlusion and resultant cerebral ischemia; none of five animals pretreated with indomethacin and infused with prostacyclin (PGI2) were clinically affected. Light- and electronmicroscopic studies showed arterial platelet-fibrin thrombi and ischemic brain damage in untreated rats. All rats had received radiolabeled platelets; radioactivity was increased in the electrogenically injured left carotid arteries from treated and untreated rats, but counts were reduced by more than 80% in indomethacin/PGI2-treated rats (p less than 0.01).
D000818:Animals; D002339:Carotid Arteries / Q000502:physiology; D004195:Disease Models, Animal; D004597:Electroshock; D011464:Epoprostenol / Q000494:pharmacology / Q000627:therapeutic use*; D007213:Indomethacin / Q000494:pharmacology / Q000627:therapeutic use*; D002546:Ischemic Attack, Transient / Q000209:etiology / Q000517:prevention & control*; D008026:Ligation; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects; D011453:Prostaglandins / Q000627:therapeutic use*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D011453:Prostaglandins; D011464:Epoprostenol; D007213:Indomethacin