The effect of corticosteroid-binding globulin on the in vitro incorporation of thymidine into human lymphocytes.
The purpose of these studies was to examine the inhibitory effect of human corticosteroid-binding globulin (CBG) on the mitogenic effect of phytohemagglutinin on cultured human lymphocytes. We found that CBG could inhibit the incorporation of [14C] thymidine into lymphocytes, but the observed inhibition could be accounted for by the cortisol which was bound to the purified CBG, CBG stripped of cortisol or bound to 11-deoxycortisol was inactive in this assay.
D002478:Cells, Cultured; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism*; D014156:Transcortin / Q000378:metabolism / Q000494:pharmacology*
D010835:Phytohemagglutinins; D014156:Transcortin; D013936:Thymidine; D006854:Hydrocortisone