Specificity of mouse hybridoma antibodies to DNA.
Utilizing the somatic cell hybridization technique, we established 12 clones of mouse hybridoma from MRL/Mp-lpr/lpr(MRL/1) and NZB X NZW(B/W F1) mice, which produce antibodies reacting mainly with ssDNA (anti-ssDNA) and 2 clones from B/W F1 mice, which produce antibodies reacting with both ssDNA and dsDNA (anti-ss/dsDNA). By means of a competitive inhibition radioimmunoassay with synthetic polynucleotides, the anti-ssDNA antibodies were classified into 5 types, in terms of the DNA base specificity. The anti-ss/dsDNA antibodies had polyspecific reactivity to a wide range of polynucleotides, including synthetic RNA.
D000818:Animals; D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002417:Cattle; D003429:Cross Reactions; D004247:DNA / Q000276:immunology*; D004277:DNA, Single-Stranded / Q000276:immunology; D006825:Hybridomas / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D011089:Polydeoxyribonucleotides / Q000276:immunology; D011863:Radioimmunoassay
D001323:Autoantibodies; D004277:DNA, Single-Stranded; D011089:Polydeoxyribonucleotides; D004247:DNA