title: Cryopreservation of the human multipotent stem cell.
abstract: Light density human bone marrow cells were cryopreserved, then thawed and tested for their ability to form myeloid (CFUGM), erythroid (BFUE) and mixed myeloid-erythroid colonies in vitro. Proliferation of mixed hematopoietic colonies was supported by conditioned media from a human T-lymphocyte cell line and erythropoietin. All mixed colonies contained neutrophil and erythroid precursors and approximately 25% had mononuclear phagocytes. Following cryopreservation the bone marrow retained its capacity to form mixed colonies without apparent loss. The recovery of CFUGM and BFUE was 76% and 72% respectively.
mesh_terms: D001853:Bone Marrow / Q000502:physiology; D001854:Bone Marrow Cells; D002455:Cell Division; D002470:Cell Survival; D003114:Colony-Forming Units Assay; D004912:Erythrocytes / Q000166:cytology / Q000502:physiology; D005615:Freezing; D006098:Granulocytes / Q000166:cytology / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000502:physiology; D011309:Preservation, Biological