[Mannan localization with concanavalin A in conjunction with electron microscopy and chemical analysis of differently prepared cell walls of the food protein yeast Candida spec. H].
Regions of different electron densities after concanavalin A-peroxidase-reaction have been observed in cell walls of intact cells as well as in isolated cell walls: In the peripheral region of intact cell walls two mannan layers appear. Treating isolated cell wall with pronase, NaOH, ethylendiamine or citrate buffer, respectively, we find on A binding spots in the inner cell wall regions which appear electron transparent in the untreated cell walls. Apparently the mannan component of the inner cell wall regions is masked by proteins. In correspondence with chemical analysis our results cannot confirm the existence of inner cell wall regions which are free of mannan.
D002175:Candida / Q000032:analysis* / Q000648:ultrastructure; D002458:Cell Fractionation; D002473:Cell Wall / Q000032:analysis / Q000648:ultrastructure; D003208:Concanavalin A / Q000378:metabolism; D005656:Fungal Proteins / Q000032:analysis; D008351:Mannans / Q000032:analysis*; D008854:Microscopy, Electron; D011134:Polysaccharides / Q000032:analysis*
D005656:Fungal Proteins; D008351:Mannans; D011134:Polysaccharides; D003208:Concanavalin A
Mannan-Lokalisation durch Concanavalin A im Zusammenhang mit elektronenmikroskopischen und chemisch-analytischen Untersuchungen an unterschiedlich präparierten Zellwänden der Futtereiweisshefe Candida spec. H.