Heterogeneity of porcine somatotropin.
A chromatographical method is presented by which highly purified porcine somatotropin can be separated preparatively into five subfractions. The rechromatographed subfractions show comparable biological activities and molecular sizes, but they differ characteristically in the polyacrylamide gel electrophoresis and in the isoelectric focusing. Relative small differences are found in the amino acid composition of these fractions. The question is discussed, whether the term multiple forms or isohormones of porcine somatotropin is suitable for the growth promoting fractions.
D000595:Amino Acid Sequence; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D013006:Growth Hormone / Q000032:analysis* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008970:Molecular Weight; D013552:Swine; D013696:Temperature
D013006:Growth Hormone