Two-dimensional gel electrophoresis of human brain proteins. III. Genetic and non-genetic variations in 145 brains.
To determine the frequency of genetic mutations, polymorphisms, and non-genetic variation in the major human brain proteins, I examined, by equilibrium two-dimensional gel electrophoresis, 145 brains from patients dying of a wide variety of psychiatric, neurological, and non-neurological disorders. Of 176 polypeptides screened, there was one polymorphism of glial fibrillary acidic protein (GFAP-Duarte). Chi square analysis indicated it was non-randomly distributed among different diseases. A possible mutation associated with Joseph's disease is being further investigated. Three examples of a possible mutation of protein 8c:1 were noted. No other genetic mutations were observed. This low frequency of polymorphisms is consistent with results for two-dimensional gel analysis of other tissue and species. The numerous non-genetic variations are described.
D001923:Brain Chemistry; D001927:Brain Diseases / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005904:Glial Fibrillary Acidic Protein; D006801:Humans; D007525:Isoelectric Focusing; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000235:genetics; D009497:Neurotic Disorders / Q000235:genetics / Q000378:metabolism; D010455:Peptides / Q000032:analysis; D011110:Polymorphism, Genetic
D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D010455:Peptides