Evaluation of a thrombin-sensitive fluorogenic substrate for heparin concentration in plasma.
Heparin levels in 76 heparinized patient plasma samples were determined with a thrombin-sensitive fluorogenic substrate. Results were compared with heparin values derived from an activated partial thromboplastin time test. The correlation of heparin concentrations obtained by these two methods was poor (r = 0.56). The amidolytic assay gave higher heparin values than the clotting method. The discrepancy is attributed to factors inherent to the methodology of these assay systems.
D000818:Animals; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D006493:Heparin / Q000097:blood*; D006801:Humans; D010314:Partial Thromboplastin Time; D010795:Phthalic Acids / Q000378:metabolism; D013917:Thrombin / Q000378:metabolism*
D010795:Phthalic Acids; C033920:phenylalanyl-prolyl-arginyl-5-amidoisophthalic acid dimethyl ester; D006493:Heparin; D013917:Thrombin