Subcellular localization of increased incorporation of [3H]fucose following passive avoidance learning in the chick.
The effect of training on a passive avoidance test on incorporation of [3H]fucose into subcellular fractions of the anterior forebrain roof of 1-day-old chicks was determined. Isotope was injected intraperitoneally, and birds tested and killed after 3 h. There was an increase in incorporation in trained compared with untrained control birds in the synaptic membrane (19% elevation, P less than 0.02) and the mitochondrial (14% elevation, P less than 0.05) fractions. Following intracranial injection, increased incorporation in trained birds was seen only in the synaptic membrane fraction (39% elevation, P less than 0.01); this increase in incorporation was not limited to any particular electrophoretically separated glycopeptides, but occurred in all 9 detected peaks.
D000818:Animals; D001362:Avoidance Learning / Q000502:physiology*; D001921:Brain / Q000378:metabolism; D002645:Chickens / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005643:Fucose / Q000378:metabolism*; D013570:Synaptic Membranes / Q000378:metabolism*; D014316:Tritium
D014316:Tritium; D005643:Fucose