Characterization of high affinity dopamine uptake into the dopamine neurons of the hypothalamus.
The study of hypothalamic dopamine (DA) neurons is complicated by the difficulty in distinguishing DA neurons from norepinephrine (NE) neurons and by the fact that they comprise only a small proportion of the catecholamine neuron population of the hypothalamus. We have studied DA uptake into nerve terminals of hypothalamic DA neurons using a synaptosomal preparation. Desmethylimipramine (DMI) was used to prevent uptake into synaptosomes from NE neurons, thus pharmacologically isolating dopaminergic from noradrenergic nerve terminals. This DMI-insensitive DA uptake in hypothalamus had all the properties of a high affinity uptake process; it was saturable, dependent on incubation time and incubation temperature, increased linearly with increasing amounts of tissue and was completely abolished by excess unlabeled DA. Also, it was completely abolished by benztropine, an inhibitor of amine uptake into DA neurons. We believe that DMI-insensitive DA uptake into hypothalamic synaptosomes represents uptake into DA nerve terminals. The DMI-insensitive DA accumulation in discrete areas of hypothalamus correlated well with the known prevalence of DA neurons relative to NE neurons in these areas: median eminence greater than median eminence-arcuate nucleus greater than mediobasal hypothalamus greater than whole hypothalamus. Comparison of the affinity constants for DA uptake into synaptosomes incubated without DMI revealed a 2-fold higher affinity constant for DA uptake in median eminence compared with striatum, but affinity constants in all the other hypothalamic regions examined (mediobasal hypothalamus, hypothalamus minus median eminence, whole hypothalamus) were identical to that of striatum. In contrast, comparison on the affinity constants for DA uptake in the presence of DMI revealed a 2-3-fold higher affinity constant for DA uptake in all these hypothalamic regions compared with striatum. It appears the tuberoinfundibular DA neurons and the other DA neurons of the hypothalamus have a high affinity uptake system for DA, although affinity for DA in all of these hypothalamic DA neurons appears to be 2-3-fold lower than that in striatal DA neurons. The data also suggest that the much larger mediobasal hypothalamus may serve as a model for studies of DA uptake into tuberoinfundibular DA neurons of the median eminence.
D000818:Animals; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D003891:Desipramine / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine / Q000187:drug effects / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism
D011954:Receptors, Dopamine; D003891:Desipramine; D004298:Dopamine; D009638:Norepinephrine