title: Spontaneous mutation rates in continuous cultures: the effect of some environmental factors.
abstract: The rate of spontaneous mutation from tryptophan auxotrophy to prototrophy has been determined under different growth-limiting and environmental conditions in continuous cultures of Escherichia coli WP2. The growth rate, aeration rate, pH and temperature were found to affect the rate of mutation. In glucose-limited cultures the mutation rate was found to be directly proportional to both the growth rate and the aeration rate. A temperature coefficient of 2 was observed and there was an optimum temperature and pH for mutation.
mesh_terms: D004734:Energy Metabolism; D004777:Environment; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D010100:Oxygen; D013696:Temperature; D014364:Tryptophan / Q000235:genetics
chemical_list: D014364:Tryptophan; D010100:Oxygen