Improved procedure for the separation of phospholipids by high-performance liquid chromatography.
We describe a rapid and efficient high-performance liquid chromatography procedure for the separation of phospholipids. The separation is accomplished on a microparticulate silica gel column using isocratic elution and UV detection at 203 nm. The solvent mixture is acetonitrile--methanol--85% phosphoric acid(130:5:1.5, v/v). Complete separation is achieved within 30 min of phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, phosphatidyldimethylethanolamine, lysophosphatidylethanolamine, phosphatidylcholine, lysophosphatidylcholine and sphingomyelin. The method is suitable for the analysis of phospholipids in tissue extracts.
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D008099:Liver / Q000032:analysis; D008297:Male; D010743:Phospholipids / Q000097:blood / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains
D010743:Phospholipids