title: Signal processing electronics for multiple electronic and optical measurements on cells.
abstract: Processing electronics for flow cytometry applications requiring simultaneous or sequential analysis of multiple electrical and optically sensed signals has been developed. A maximum of six analog signals are input to the processor. A measurement mode selector determines what signals are to be analyzed and the initial timing sequence of sense gates for acquiring signal crest values. Processor signal-triggering and sense gate time delays are selectable. Logic coincidence-anticoincidence circuits determine constraints on incoming signals. Gated peak-sense and hold signals are routed to computer interface electronics for digitizing and are then displayed as frequency distribution histograms using an LSI-11 computer. Signals also are processed as single parameters, ratios, and gated single parameters for output to a multichannel pulse-height analyzer and cell sorting electronics. The functional features of the processor are described along with examples illustrating simultaneous and sequential analysis of cultured cells stained with fluorescent dyes.
mesh_terms: D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002469:Cell Separation / Q000295:instrumentation*; D003201:Computers; D006224:Cricetinae; D003593:Cytoplasm / Q000648:ultrastructure; D004247:DNA / Q000032:analysis; D004581:Electronics; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation*; D010053:Ovary; D011506:Proteins / Q000032:analysis
chemical_list: D011506:Proteins; D004247:DNA