Deuterium isotope effects in the ninhydrin reaction of primary amines.
The rate of development of Ruhemann's purple in the ninhydrin reaction of two deuterated primary amines, aa-d2-p-tyramine and aa-d2-beta-phenylethylamine, is significantly reduced. It appears to be a primary isotope effect and indicates that the cleavage of the carbon-hydrogen bond at the a-position is involved in the rate-determining step of the color reaction.
D000588:Amines / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis; D003124:Colorimetry / Q000379:methods; D003903:Deuterium; D007192:Indenes; D007700:Kinetics; D009555:Ninhydrin
D000588:Amines; D000596:Amino Acids; D007192:Indenes; D003903:Deuterium; D009555:Ninhydrin