The multi-residue determination of coumarin-based anticoagulant rodenticides in animal materials by high-performance liquid chromatography.
The rodenticides brodifacoum, difenacoum, coumatetralyl and warfarin are determined in animal relicta by high-performance exclusion chromatography on porous silica. The first three compounds are not separated, but are subsequently differentiated by adsorption or reversed-phase high-performance liquid chromatography of the appropriate eluate fraction collected from the exclusion column. The method is rapid, and clean-up (on Sep-Pak silica cartridges) is simple. Mean recoveries from spiked substrates were generally above 80% at levels of 0.1-1.0 mg/kg. Routine limits of determination are about 0.05-0.1 mg/kg for warfarin and about 0.02 mg/kg for the other compounds. If analysis for warfarin is not required, the latter limit can be lowered to about 1 microgram/kg by a slight modification to the clean-up step.
D015110:4-Hydroxycoumarins / Q000032:analysis*; D000818:Animals; D000925:Anticoagulants; D002851:Chromatography, High Pressure Liquid; D008099:Liver / Q000032:analysis; D012016:Reference Values; D012378:Rodenticides / Q000097:blood* / Q000652:urine; D013270:Stomach / Q000032:analysis; D013552:Swine; D014859:Warfarin / Q000032:analysis
D015110:4-Hydroxycoumarins; D000925:Anticoagulants; D012378:Rodenticides; D014859:Warfarin; C013418:bromfenacoum; C004699:coumatetralyl; C012903:difenacoum