[Extractive polarographic method of controlling the stereospecific hydrolysis of N-acylated derivatives of amino acid racemates].
The equilibrium distribution of acetyl-D-phenylglycine prepared with stereospecific hydrolysis of acetyl-D,L-phenylglycine (Ac-D,L-PhG) was studied in a two-phase system of benzyl alcohol-1N HCl solution. It was found that the distribution coefficient of Ac-D-PhG did not practically depend on its concentration in the aqueous phase. This means that Ac-D-PhG does not form associates in the organic phase. The studies provided development of an extraction polarimetric method for quantitative determination of Ac-D-PhG. The method implies extraction of Ac-D-PhG with benzyl alcohol from a strong acid medium (1N HCl solution), polarimetric determination of the equilibrium concentration of Ac-D-PhG in the organic phase and subsequent calculation of the initial concentration of Ac-D-PhG in the aqueous phase with regard to its distribution coefficient.
D000215:Acylation; D000596:Amino Acids / Q000032:analysis / Q000302:isolation & purification*; D005998:Glycine / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification; D006868:Hydrolysis; D008433:Mathematics; D011048:Polarography / Q000379:methods*; D013237:Stereoisomerism
D000596:Amino Acids; C027177:N-acetylphenylglycine; D005998:Glycine
Ekstraktsionno-poliarimetricheskiĭ metod kontrolia stereospetsificheskogo gidroliza N-atsilirovannykh proizvodnykh ratsematov aminokislot.