Adenylate cyclase and exocytosis of membrane-coating granules in oral mucosa.
Adenylate cyclase-cAMP system has been demonstrated to be important in the regulation of proliferation and growth of stratified squamous epithelium. This study was performed to localize adenylate cyclase in the oral mucosa of the rabbit and to study the effect of isoproterenol on exocytosis of membrane-coating granules. Cytochemical localization of adenylate cyclase revealed that the enzyme was on the cell membrane of some basal and parabasal cells, most upper spinous cells, and an occasional dense superficial cell. The specific distribution of this enzyme in the stratified squamous epithelium is deemed to be related to the regulation of cell proliferation in the basal and parabasal layers, and related to the exocytosis of membrane-coating granules in the upper spinous layer. Electron-microscopic morphometric quantification of membrane-coating granules showed that there was decreased concentration of membrane-coating granules in isoproterenol-treated animals, indicating there is an accelerated exocytosis of granules upon stimulation of the adenylate cyclase-cAMP system by isoproterenol.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D005089:Exocytosis / Q000187:drug effects; D006651:Histocytochemistry; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008854:Microscopy, Electron; D009061:Mouth Mucosa / Q000502:physiology* / Q000648:ultrastructure; D011817:Rabbits
D000262:Adenylyl Cyclases; D007545:Isoproterenol