Absence of preferential glutamine uptake into neurons--an indication of a net transfer of TCA constituents from nerve endings to astrocytes?
Uptake kinetics for glutamine were studied in several different neuronal preparations (perikarya prepared by gradient centrifugation, cultured cortical neurons, cultured, presumably glutamatergic cerebellar neurons, and brain prisms). In no case were any indications found of a high affinity uptake but a rather efficient low affinity uptake did occur. A similar, equally efficient low affinity uptake is, however, found in astrocytes. Thus, no preferential glutamine uptake occurs into neurons. It is, therefore, not likely that a net flow of glutamine takes place from astrocytes to neurons, compensating for the loss of TCA constituents when glutamate and GABA are released.
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001692:Biological Transport; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D008811:Mice, Inbred DBA; D009474:Neurons / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism; D014233:Tricarboxylic Acids / Q000378:metabolism*
D014233:Tricarboxylic Acids; D005973:Glutamine