Simple assay for urinary iron after desferrioxamine therapy.
A simple method for measuring urinary iron following the administration of desferrioxamine (DF) is described. Ferrioxamine iron is reduced by a reagent containing thioglycolic, hydrochloric, and trichloroacetic acids; the latter ensuring precipitation of any protein present in occasional urines. The iron is measured spectrophotometrically using bathophenanthroline sulfonate. Results are indistinguishable from those obtained using a much more time-consuming preliminary acid digestion, except that in baseline samples the very small amount of spontaneous iron excretion is underestimated. The method is suitable for routine use in monitoring DF therapy.
D000747:Anemia, Hypochromic / Q000188:drug therapy* / Q000652:urine; D003676:Deferoxamine / Q000627:therapeutic use*; D006801:Humans; D007501:Iron / Q000652:urine*; D008722:Methods; D008991:Monitoring, Physiologic; D012016:Reference Values; D013053:Spectrophotometry
D007501:Iron; D003676:Deferoxamine