title: Lipofuscin: resolution of discrepant fluorescence data.
abstract: Lipofuscin granules (age pigments) emit yellow light under ultraviolet excitation in the fluorescence microscope. The reported blue emission maximum of extracts of lipofuscin-laden cells may result from instrumental bias. The major fluorescent components that accumulate with age in these lysosomal residual bodies of human retinal pigment epithelium are yellow-emitting fluorophores. Different age-related fluorophores, which do emit blue light, are derived from other intracellular sources. A reevaluation of the connection between blue-emitting lipid peroxidation products and the age-related lipofuscin granules of classical pathology is necessary.
mesh_terms: D000328:Adult; D000367:Age Factors; D003594:Cytoplasmic Granules; D006801:Humans; D008062:Lipofuscin; D008247:Lysosomes / Q000032:analysis; D010857:Pigment Epithelium of Eye / Q000032:analysis / Q000648:ultrastructure; D010860:Pigments, Biological; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis
chemical_list: D008062:Lipofuscin; D010860:Pigments, Biological