title: Extension of human acid alpha-glucosidase polymorphism by isoelectric focusing in polyacrylamide gel.
abstract: 1. A method to analyse acid alpha-glucosidase (GAA) activity in human tissues by flatbed polyacrylamide gel isoelectric focusing has been devised. 2. With this method the GAA polymorphism has been extended from three to six commonly occurring phenotypes. 3. After isoelectric focusing the GAA2 isozyme migrated cathodally with respect to the GAA1 isozyme, whereas with the previous starch-gel electrophoresis method their relative positions were reversed. 4. The six common phenotypes are generated by three alleles, GAA*1, GAA*2 and GAA*4 with frequencies of 0.91, 0.03 and 0.06, respectively.
mesh_terms: D000483:Alleles; D004591:Electrophoresis, Polyacrylamide Gel; D004592:Electrophoresis, Starch Gel; D005260:Female; D005959:Glucosidases / Q000235:genetics*; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007962:Leukocytes / Q000201:enzymology; D010641:Phenotype; D010920:Placenta / Q000201:enzymology; D011110:Polymorphism, Genetic; D011247:Pregnancy; D000520:alpha-Glucosidases / Q000235:genetics* / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D005959:Glucosidases; D000520:alpha-Glucosidases