Membrane-active agents and heat-induced erythrocyte fragmentation.
Morphological observations of heated erythrocytes were made after preincubation with lysolecithin, diamide, p-chloromercuribenzoate, or N-ethyl maleimide and after ATP-depletion. Diamide (1.0 mM) reduced the critical temperature of fragmentation from 49 degrees C to 47 degrees C, and at higher concentrations the critical temperature was further reduced. The other sulphydryl reacting agents had little or no effect. Drug-induced spherocytes and ATP-depleted cells did not fragment. Membrane-active agents can effect the response of membranes to heat and the effect is dose-dependent.
D000255:Adenosine Triphosphate / Q000097:blood; D002729:Chloromercuribenzoates / Q000494:pharmacology; D002746:Chlorpromazine / Q000494:pharmacology; D003958:Diamide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004910:Erythrocyte Membrane / Q000187:drug effects; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects; D005033:Ethylmaleimide / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D020245:p-Chloromercuribenzoic Acid
D002729:Chloromercuribenzoates; D008244:Lysophosphatidylcholines; D003958:Diamide; D020245:p-Chloromercuribenzoic Acid; D000255:Adenosine Triphosphate; D005033:Ethylmaleimide; D002746:Chlorpromazine