title: Stability of frozen liquid control sera for assay of cholesterol in high-density lipoprotein.
abstract: Liquid serum pools with low-, normal-, or above-normal concentration of high-density lipoprotein cholesterol were prepared by selection and dilution of sera having low lipoprotein content or by enrichment with concentrated high-density lipoprotein. Stability of the serum pools depended on storage conditions. On storage between -2 and -12 degrees C, the high-density lipoprotein cholesterol content decreased. At a constant temperature of -19 to -21 degrees C the concentrations remained stable for more than 18 months. The imprecision (CV) of the high-density lipoprotein cholesterol assay during this period as established with enzymic cholesterol analysis (Clin. Chem. 26: 1780-1786, 1980) of these serum pools was between 2.7 and 4.8% (n = 51). Serum pools prepared and stored as described are suitable for internal quality-control procedures. In external quality-control trials these sera may be superior to the commercially available lyophilized lipid control sera.
mesh_terms: D001793:Blood Preservation; D002784:Cholesterol / Q000032:analysis*; D004796:Clinical Enzyme Tests; D003080:Cold Temperature; D005615:Freezing; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D011786:Quality Control; D012015:Reference Standards; D013997:Time Factors; D014280:Triglycerides / Q000097:blood
chemical_list: D008075:Lipoproteins, HDL; D014280:Triglycerides; D002784:Cholesterol