title: Microscale multisample two-dimensional electrophoresis of proteins in human serum, cerebrospinal fluid, and urine.
abstract: In this technique, in which no denaturing agent is used, proteins in human serum, cerebrospinal fluid, and urine are separated by isoelectric focusing in cylindrical 40 g/L polyacrylamide gels of capillary size (1.3 x 35 mm) for 40 min, followed by electrophoresis in 40--170 g/L polyacrylamide linear gradient gel, with use of 38 x 35 x 1 mm slab gel, for 1 h. Only 2 microL of untreated human serum is required to obtain clear protein-distribution patterns, made visible by Coomassie Blue staining. By use of silver staining, proteins in unconcentrated cerebrospinal fluid can be made visible. An apparatus we devised for microscale two-dimensional electrophoresis enables us to analyze eight protein samples simultaneously.
mesh_terms: D001798:Blood Proteins / Q000032:analysis*; D002556:Cerebrospinal Fluid Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing / Q000295:instrumentation* / Q000379:methods; D009393:Nephritis / Q000652:urine; D011507:Proteinuria; D012016:Reference Values
chemical_list: D001798:Blood Proteins; D002556:Cerebrospinal Fluid Proteins