Chemical fingerprinting of glycosphingolipids in meconium of a human blood group O Le(a-b+) secretor.
Studying blood group polymorphism, as expressed in intestinal tissue of single individuals, total non-acid glycosphingolipids of meconium of individual human newborns have been prepared. Silicic acid column chromatography of the acetylated derivatives were used for a stepwise separation into four groups of glycolipids from each individual meconium. By the combined use of mass spectrometry and NMR spectroscopy of permethylated and LiA1H4-reduced, permethylated derivatives and by immunology of the native fractions all the major glycolipids were identified, although in mixtures. The interest was focused on fucolipids known to be strictly regulated by the ABO, H, Le and Se genes. The fucosylated glycolipids of an O Le (a-b+) secretor child were dominated by blood group H-active and Lewis-active mon- and difucosyl compounds with 5-6 sugar residues and having a core lactotetraosyl structure. The lipophilic part was dominated by 2-hydroxy fatty acids with 16 and 20-24 carbon atoms bounds to either sphingosine of phytosphingosine.
D000017:ABO Blood-Group System / Q000235:genetics*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006028:Glycosphingolipids / Q000032:analysis*; D006801:Humans; D007106:Immune Sera; D007231:Infant, Newborn; D037102:Lectins; D007983:Lewis Blood Group Antigens / Q000235:genetics*; D013058:Mass Spectrometry; D008470:Meconium / Q000032:analysis*; D011110:Polymorphism, Genetic
D000017:ABO Blood-Group System; D006028:Glycosphingolipids; D007106:Immune Sera; D037102:Lectins; D007983:Lewis Blood Group Antigens