Transfer properties of the bovine brain phospholipid transfer protein. Specificity towards phosphatidylcholine analogs and the inhibitory effect of sphingomyelin.
A coupled transport of phosphatidylinositol from the monolayer to phosphatidylcholine vesicles, and a phosphatidylcholine transport in the reverse direction in the presence of bovine brain transfer protein is demonstrated. No significant amounts of protein accumulate at the interface during the transfer reaction. The transfer protein from bovine brain shows a lower specificity for phosphatidylcholine than does the transfer protein from bovine liver. Relative to egg phosphatidylcholine a low transfer rate is found for derivatives with a chain length of 14 carbon atoms and a distance between phosphorus and nitrogen of 6 carbon atoms. The gel state of phosphatidylcholine does not reduce the transfer reaction as catalyzed by the bovine brain protein. The transfer of phosphatidylinositol is inhibited by sphingomyelin. The presence of 200 mM K+ or 1mM Ca2+ does not affect the transfer activity of the bovine brain protein. Divalent ions at concentrations higher than 5 mM cause a fusion of vesicles with monolayers. The pH optimum of the phosphatidylinositol transfer reaction is 8.
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002417:Cattle; D002540:Cerebral Cortex / Q000378:metabolism*; D007700:Kinetics; D008565:Membrane Proteins; D008862:Microsomes, Liver / Q000378:metabolism; D009994:Osmolar Concentration; D010713:Phosphatidylcholines / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism; D049294:Phospholipid Transfer Proteins; D051381:Rats; D013109:Sphingomyelins / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D002352:Carrier Proteins; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D049294:Phospholipid Transfer Proteins; D013109:Sphingomyelins