title: Structural changes in alkaline-treated postsynaptic membranes from Torpedo marmorata are not due to lipid hydrolysis.
abstract: The removal of the 43 kDa peptide from postsynaptic membranes from the electric organ of Torpedo by alkaline treatment (pH 11) or by lithium diiodosalicylate at pH 8 results in similar ultrastructural changes that cannot be produced by the action of porcine pancreatic phospholipase A2 or lysophosphatidylcholine. Thin-layer chromatography fails to reveal significant alkaline hydrolysis of membrane lipids from postsynaptic membranes and erythrocyte ghosts under the conditions used for peptide extraction.
mesh_terms: D000818:Animals; D004557:Electric Organ / Q000648:ultrastructure; D006868:Hydrolysis; D008563:Membrane Lipids / Q000032:analysis*; D008565:Membrane Proteins / Q000302:isolation & purification; D008970:Molecular Weight; D011950:Receptors, Cholinergic / Q000032:analysis; D013570:Synaptic Membranes / Q000648:ultrastructure*; D014101:Torpedo
chemical_list: D008563:Membrane Lipids; D008565:Membrane Proteins; D011950:Receptors, Cholinergic