Dynorphin-related immunoreactive peptides in rat brain and pituitary.
Antisera recognizing dynorphin 1-13, but not other opioid peptides with a high avidity, have been generated in rabbits. Using a radioimmunoassay technique levels of dynorphin-immunoreactivity (dyn-ir) have been measured in the brain and pituitary of rats. The concentrations of dyn-ir ranged from a maximum in the intermediate/posterior lobe of the pituitary (about 1200 pmol/g) to a minimum in the cerebellum (about 1 pmol/g) as follows: pituitary intermediate/posterior lobe greater than adenohypophysis greater than hypothalamus greater than hippocampus = striatum = midbrain = thalamus = medulla/pons greater than cortex greater than cerebellum. Gel-filtration of hypothalamic extracts revealed 4 immunoreactive components with apparent molecular weights (MW) of 3500, 2400, 1300 and less than 1000 daltons, respectively. No dyn-ir was found to elute as dynorphin1-13 (MW = 1700). The 2400 and 1300 dalton materials showed opiate-like activity on the guinea-pig ileum longitudinal muscle preparation, indicating that a substantial part of the dyn-ir measured represented biologically active material.
D000818:Animals; D001923:Brain Chemistry; D003342:Corpus Striatum / Q000032:analysis; D004399:Dynorphins; D004723:Endorphins / Q000032:analysis*; D006624:Hippocampus / Q000032:analysis; D007031:Hypothalamus / Q000032:analysis; D008297:Male; D010446:Peptide Fragments / Q000032:analysis*; D010902:Pituitary Gland / Q000032:analysis*; D010903:Pituitary Gland, Anterior / Q000032:analysis; D010904:Pituitary Gland, Posterior / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D004723:Endorphins; D010446:Peptide Fragments; C026843:dynorphin (1-13); D004399:Dynorphins