H4-isozyme of lactate dehydrogenase in a solution of sodium chloride-I. The molecular weight of H4-lactate dehydrogenase in relation to sodium chloride concentration, enzyme concentration and temperature.
1. The behavior of H4-lactate dehydrogenase in a solution of sodium chloride was studied with respect to its molecular weight. The molecular weight decreased as the concentration of sodium chloride increased. 2. On a controlled-pore glass column equilibrated by 0.5 M soldium chloride, H4-lactate dehydrogenase was found to have mol. wt 77,500. 3. In a low salt column (0.1 M sodium chloride or less), on the other hand, the molecular weight was found to be about 135,000. 4. Salt concentration dependent association-dissociation system of the enzyme was also observed by the sedimentation equilibrium method.
D000818:Animals; D002417:Cattle; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008970:Molecular Weight; D009994:Osmolar Concentration; D011817:Rabbits; D012965:Sodium Chloride; D013696:Temperature; D014461:Ultracentrifugation
D007527:Isoenzymes; D012965:Sodium Chloride; D007770:L-Lactate Dehydrogenase