Affinity chromatography purification of Pseudomonas aeruginosa exotoxin A on specifically linked NAD agarose.
The specific binding of P. aeruginosa exotoxin A to NAD was exploited for the rapid purification of the toxin. Affinity chromatography on a column of agarose-N6-(aminohexyl)carbamoylmethyl-NAD resulted in an enzymatically, biologically, and immunologically active purified toxin preparation. Other NAD-agarose resins were not efficient substrates for toxin purification.
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D002846:Chromatography, Affinity; D005098:Exotoxins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D009243:NAD / Q000378:metabolism; D012685:Sepharose; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D005098:Exotoxins; D009243:NAD; D012685:Sepharose; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A