The molecular structure of d(ICpCpGpG), a fragment of right-handed double helical A-DNA.
The DNA tetramer d(ICpCpGpG) or ICCGG crystallizes as a double-stranded 4-base pair (bp) segment of an A helix. Two such tetramer helices are packed together in the crystal with local helix axes nearly coincident, simulating an 8-bp helix, and four such octamers make up the tetragonal unit cell. Restrained energy and reciprocal space refinement has led to an R factor of 20.5% at 2.1 A resolution. The ICCGG helix has a twist corresponding to 10.7 bp per turn, a 19 degree base tilt and a 2.3 A rise per base pair along the helix axis. The mean propeller twist of 18 degree is comparable with, and has the same rotational sense as that observed in the B-DNA dodecamer CGCGAATTCGCG at similarly high alcohol concentration. Backbone phosphate groups in A-DNA are extensively hydrated, including a network across the opening of the major groove, whereas base edge N and O groups in major and minor grooves are less hydrated than in B-DNA. The minor groove spine of hydration observed in B-DNA is totally absent. These observations of relative hydration confirm and extend the model for the B- to A- helix transition proposed earlier on the basis of the B helix structure.
D001482:Base Composition; D004247:DNA; D046911:Macromolecular Substances; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D013329:Structure-Activity Relationship
D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; C033718:di-(deoxycytidylic-deoxyguanylic acid); D004247:DNA