Reversibility of the insulin-stimulated phosphorylation of ATP citrate lyase and a cytoplasmic protein of subunit Mr 22000 in adipose tissue.
Exposure of rat epididymal adipose tissue to insulin leads to a 2-fold increase in incorporation of [32P]phosphate into ATP citrate lyase and a cytoplasmic protein of Mr 22000. A smaller increase and decrease in incorporation into cytoplasmic proteins of Mr 63000 and 20000 respectively were also observed. Subsequent addition of anti-insulin serum largely reversed these changes within 15 min, indicating that the alterations in 32P incorporation represented changes in net phosphate content of the proteins.
D001275:ATP Citrate (pro-S)-Lyase / Q000378:metabolism*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004837:Epinephrine / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D007330:Insulin Antibodies; D008297:Male; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical
D007328:Insulin; D007330:Insulin Antibodies; D010750:Phosphoproteins; D001275:ATP Citrate (pro-S)-Lyase; D004837:Epinephrine