title: The effect of the cryoprotectants, dimethylsulfoxide and glycerol on water transport in the human red blood cell.
abstract: The response of human red blood cells to the cryoprotective agents, DMSO and glycerol, has been investigated using a pulsed NMR method. The experimentally determined parameters are: (1) the intracellular transverse relaxation time, T2a; (2) the mean residence time of intracellular water, tau a, which is effectively a reciprocal measure of the rate of water transport across the red blood cell membrane; and (3) the activation energy for this process. The quantitative data indicate that the observed effects are colligative rather than species-specific in origin.
mesh_terms: D001692:Biological Transport; D001834:Body Water / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005990:Glycerol / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy
chemical_list: D005990:Glycerol; D004121:Dimethyl Sulfoxide