The effect of chemical and physiological factors on the kinetics for product formation as it relates to enzyme activity and concentration, reaction time and to substrate adsorption and affinity.
1. The dependency of product formation on reaction time and on enzyme concentration was studied with certain purified enzymes and liver microsomal mixed-function oxidase system. 2. The product-formation-time relation was estimated with different enzyme and/or substrate concentrations for the reactions limited by diffusion. 3. The kinetic constants of the product-time relations in these diffusion systems have been verified experimentally to give a more specific and detailed characterization of an enzyme system under a variety of physiologic and reaction conditions. 4. The influence of inhibitors and activators in vitro, the effect of enzyme preparation technic, aging, starvation, pretreatment with somatotropic hormone, phenobarbital or 3-methylcholanthrene, and the effect of a tumor were studied. 5. The influence of vitamin C deficiency in guinea pig was also studied.
D000230:Adenocarcinoma / Q000201:enzymology; D000818:Animals; D001206:Ascorbic Acid Deficiency / Q000201:enzymology; D004789:Enzyme Activation; D004791:Enzyme Inhibitors; D004798:Enzymes / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D007700:Kinetics; D008297:Male; D008433:Mathematics; D008748:Methylcholanthrene / Q000494:pharmacology; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology*; D008956:Models, Chemical; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D013217:Starvation / Q000201:enzymology; D013329:Structure-Activity Relationship; D013997:Time Factors
D004791:Enzyme Inhibitors; D004798:Enzymes; D008748:Methylcholanthrene; D010634:Phenobarbital