[A detailed lipidograph: enzymatic determination of cholesterol, phospholipids and glycerides in plasma lipoprotein after a cellulose acetate electrophoretic procedure (author's transl)].
The specific determination of cholesterol and its evaluation in lipoproteins separated by cellulose acetate electrophoresis is described. Using this technique, the authors extended the specific lipid determinations to phospholipids and glycerides. The precision, linearity, accuracy of the methods are satisfactory for fractions greater than 10%. Phospholipids and cholesterol values in alpha lipoproteins are compared with those obtained in the supernatant by concanavalin A precipitation; the differences between the two methods are not significant. We suggest using these three enzymatic determinations to produce a detailed lipidograph. For a selected population, the lipid values in each fraction (expressed in g/l and in mmol/l) were close to those given in the literature. We compared our lipidograph, expressed in SI units, with the Oil Red O stained lipidograph; the difference was not significant.
D000328:Adult; D011232:Chemical Precipitation; D002784:Cholesterol / Q000032:analysis*; D003208:Concanavalin A; D004588:Electrophoresis, Cellulose Acetate; D005989:Glycerides / Q000032:analysis*; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male; D008722:Methods; D010743:Phospholipids / Q000032:analysis*
D005989:Glycerides; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008079:Lipoproteins, VLDL; D010743:Phospholipids; D003208:Concanavalin A; D002784:Cholesterol
Lipidogramme détaillé: évaluation enzymatique du cholestèrol, des phospholipides et des glycerides après électrophorèse du plasma sur acétate de cellulose.