Interactions of different albumins and animal sera with insolubilized Cibacron Blue, Evaluation of apparent affinity constants.
1. A high concentration Cibacron Blue-Sepharose derivative has been used to study the affinity chromatography of albumin from eight animal species. 2. The apparent affinity constants for albumin varies between 3.9 x 10(4) M-1 and 0.9 x 10(4) M-1, in the order: Human greater than rabbit greater than horse greater than pig = dog greater than bovine greater than rat greater than chicken. 3. Other serum proteins were also bound to the gel, particularly lipoproteins and alpha 2-macroglobulin.
D000818:Animals; D000873:Anthracenes; D002417:Cattle; D002645:Chickens; D004396:Coloring Agents; D004285:Dogs; D006736:Horses; D006801:Humans; D007700:Kinetics; D011485:Protein Binding; D011817:Rabbits; D051381:Rats; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D012995:Solubility; D013045:Species Specificity; D013552:Swine; D014227:Triazines
D000873:Anthracenes; D004396:Coloring Agents; D012709:Serum Albumin; D014227:Triazines; D012710:Serum Albumin, Bovine; C012842:Cibacron Blue F 3GA