Antigens of the basement membranes of the seminiferous tubules induce autoimmunity in Wistar rats.
A preparation enriched in basement membranes from seminiferous tubules was isolated from rat testes (STBM) and injected with complete Freund's adjuvant into Wistar rats. In 60% of animals a mild multifocal orchitis was observed. In damaged areas, perivascular and peritubular mononuclear cell infiltrates and different degrees of cell sloughing of some seminiferous tubules were observed. Electron microscopy revealed focal thickenings and delamination of the basement membrane of the seminiferous tubules as well as vacuolization of Sertoli cell cytoplasm. Using immunofluorescence discontinuous linear deposits of IgG were detected along the seminiferous tubular wall. Moreover, the same pattern of immunofluorescence was observed when the IgG eluted from the testes of the immunized rats was layered on sections of normal rat testis. Circulating antibodies to STBM were detected using passive haemagglutination in approximately 45% of the immunized rats, with titers ranging from 1:20 to 1:80. Leukocyte migration was inhibited when the spleen cells of the immunized rats were incubated with antigens from the basement membrane of seminiferous tubules, whilst a negative reaction was obtained when the soluble fraction of testis homogenate was used.
D000818:Animals; D000941:Antigens / Q000008:administration & dosage*; D001323:Autoantibodies / Q000096:biosynthesis* / Q000276:immunology; D001485:Basement Membrane / Q000276:immunology / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests; D007111:Immunity, Cellular; D007114:Immunization; D008297:Male; D009920:Orchitis / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D012671:Seminiferous Tubules / Q000276:immunology* / Q000648:ultrastructure; D013737:Testis / Q000276:immunology*
D000941:Antigens; D001323:Autoantibodies