Effect of therapeutic levels of magnesium sulphate, methyldopa, hydralazine and phenobarbitone on normal term human trophoblast in vitro.
The development of a technique allowing prolonged culture in vitro of normal term human trophoblast facilitates examining what effects, if any, drugs commonly used in pregnancy may have on placental tissue. Dosages compatible with therapeutic blood levels of magnesium sulphate, methyldopa, hydralazine and phenobarbitone were tested individually in vitro on monolayer cultures of normal term trophoblast. Hydralazine and magnesium sulphate additions resulted in a 100 per cent loss of viability of trophoblast cells. Phenobarbitone caused an 8 per cent decrease in cell viability, while methyldopa caused only a 2 per cent decrease in cell viability.
D005260:Female; D006801:Humans; D006830:Hydralazine / Q000009:adverse effects*; D066298:In Vitro Techniques; D008278:Magnesium Sulfate / Q000009:adverse effects*; D008750:Methyldopa / Q000009:adverse effects*; D010634:Phenobarbital / Q000009:adverse effects*; D011247:Pregnancy; D014327:Trophoblasts / Q000187:drug effects* / Q000473:pathology
D006830:Hydralazine; D008750:Methyldopa; D008278:Magnesium Sulfate; D010634:Phenobarbital