Rapid washing of filter paper discs in a solid-phase radioimmunoassay with a constant flow washing device.
A machine has been developed for the rapid washing of the cellulose filter paper discs that are used in a number of radioimmunoassays. The machine is simple in design, easy to use, and is capable of washing 96 filter paper discs simultaneously. The efficiency of the machine is demonstrated by a RAST assay for measuring IgE antibodies to the venom. Time taken to wash the discs was reduced 3-fold without loss of sensitivity or reproducibility.
D000818:Animals; D001514:Bee Venoms / Q000276:immunology; D001666:Binding Sites, Antibody; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D010209:Paper; D011852:Radioallergosorbent Test; D011863:Radioimmunoassay / Q000295:instrumentation; D013997:Time Factors
D001514:Bee Venoms; D007073:Immunoglobulin E