Determination of mimosine and 3-hydroxy-4(1h)-pyridone in Leucaena, avian excreta and serum using reversed-phase ion-pair high-performance liquid chromatography.
The estimation of mimosine and 3-hydroxy-4(1H)-pyridone in Leucaena leucocephala, Leucaena seeds, chick excreta and chick serum using reversed-phase ion-pair high-performance liquid chromatography was investigated. Isocratic elution of both compounds was achieved in 11 min using sodium octyl sulphate as the pairing agent in a pH 2.25 buffer. Good recoveries of both mimosine and 3-hydroxy-4(1H)-pyridone in all but serum samples were obtained.
D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000032:analysis; D008898:Mimosine / Q000097:blood*; D011728:Pyridones / Q000097:blood*; D012639:Seeds / Q000032:analysis
D011728:Pyridones; C020366:3-hydroxy-4-pyridone; D008898:Mimosine