title: The kinetics of acetylcholine turnover in a resting cholinergic nerve terminal and the magnitude of the cytoplasmic compartment.
abstract: The magnitude of the cytosolic pool of acetylcholine in the cholinergic electromotor nerve terminals of Torpedo marmorata has been calculated to be 22 +/- 3% of the total by comparing the isotopic ratio of acetylcholine with that of choline when slices of electric organ were incubated with 10 microM deuterated choline. The calculation is based on a two-compartment model that assumes the presence, in unstimulated tissue, of a vesicular pool of acetylcholine that does not exchange, under resting conditions, with a second cytosolic pool; the latter, by contrast, is subject to 'futile recycling' and comes into isotopic equilibrium with the tissue choline pool.
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002451:Cell Compartmentation; D002794:Choline / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004557:Electric Organ / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D009046:Motor Neurons / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000378:metabolism*; D014101:Torpedo
chemical_list: D011950:Receptors, Cholinergic; D002794:Choline; D000109:Acetylcholine