Regenerating goldfish retinal explants: induction and maintenance of neurites by conditioned medium from cells originated in the nervous system.
Fiber outgrowth from goldfish regenerating retinas can be induced by conditioned medium of cloned cells which originated in the nervous system, i.e. glioma and neuroblastoma. Dilution of the released factor(s) was required to achieve optimal effect; high concentrations are detrimental. The fibers can be maintained for at least 2 weeks in vitro, and reach a length of several millimeters. This system may provide a means to purify and characterize neurotrophic factors involved in nerve regeneration.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D003470:Culture Media; D046508:Culture Techniques; D006054:Goldfish; D009412:Nerve Fibers / Q000648:ultrastructure; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000187:drug effects*; D009457:Neuroglia / Q000166:cytology; D009474:Neurons / Q000166:cytology; D051381:Rats; D012160:Retina / Q000166:cytology*
D003470:Culture Media; D009414:Nerve Growth Factors