title: Transplantation of mouse mammary epithelial cells grown in primary collagen gel cultures.
abstract: A technique for the transplantation of mouse mammary epithelial cells, grown in collagen gels, has been developed that demonstrates that the phenotype of the cells prior to culture was not altered by the culture conditions. Mammary epithelial cells from virgin and midpregnant C57BL/Crgl mice; virgin, midpregnant, and multiparous nonpregnant BALB/cfC3H/Crgl mice; a BALB/c hyperplastic alveolar nodule line, and mammary tumors from BALB/cfC3H/Crgl mice were embedded inside collagen gels and grown for 10 to 14 days in the presence of 25% swine serum plus cholera toxin (0.01 microgram/ml). The epithelial cells increased in number during the culture period. At the end of the culture period, the cells were removed from the collagen gels and transplanted to the gland-free mammary fat pads of 3-week-old syngeneic female mice. Culture in collagen gels increased the number of cells necessary to obtain a high percentage of mammary outgrowths as compared to cells not grown inside collagen gels. In general, mammary cells grown inside collagen gels gave rise to outgrowths, similar in phenotype to those from noncultured cells, and were representative of the tissue of origin. Mammary epithelial cells from C57BL/Crgl virgin donors grown in collagen gels for 10 to 14 days retained their ability to respond to the endogenous hormones of pregnancy and lactation of the host and formed lobuloalveolar structures full of secretion similar to the host's own mammary gland. The data indicate that the growth of mammary epithelial cells in collagen gels and subsequent transplantation into the gland-free fat pads of syngeneic hosts provides a simple system, wherein cells can be grown in vitro and their phenotypes determined in vivo.
mesh_terms: D000230:Adenocarcinoma / Q000503:physiopathology; D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen; D003470:Culture Media; D004848:Epithelium / Q000637:transplantation; D005260:Female; D008321:Mammary Glands, Animal / Q000502:physiology / Q000637:transplantation*; D008325:Mammary Neoplasms, Experimental / Q000503:physiopathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D011247:Pregnancy
chemical_list: D003470:Culture Media; D003094:Collagen