Double labelling of neuronal somata by [3H]uridine and horseradish peroxidase: a technique to demonstrate two-stage connections with the light microscope.
A new technique is described that may demonstrate two-stage connection at the light microscopic level. Anterograde transneuronal transport of [3H]uridine is used in conjunction with retrograde transport of horseradish peroxidase (HRP). The two compounds are injected separately into different brain regions. Under conditions of proper controls, neuron somata that are double labelled are interpreted as receiving an afferent projection from the region injected with [3H]uridine and sending an efferent projection to the region injected with HRP. Thus, the technique can demonstrate two-stage connections in the nervous system.
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000033:anatomy & histology*; D001931:Brain Mapping / Q000379:methods*; D001933:Brain Stem / Q000033:anatomy & histology; D006735:Horseradish Peroxidase; D010296:Parietal Lobe / Q000033:anatomy & histology; D010544:Peroxidases; D051381:Rats; D013787:Thalamic Nuclei / Q000033:anatomy & histology; D014529:Uridine / Q000378:metabolism*; D014793:Visual Cortex / Q000033:anatomy & histology; D014795:Visual Pathways / Q000033:anatomy & histology
D006735:Horseradish Peroxidase; D010544:Peroxidases; D014529:Uridine