Photoaffinity labelling of the serotonin carrier protein in platelets and brain synaptosomes.
Azidoimipramine, a photoaffinity labelling reagent for the serotonin transport protein, was synthesized. This reagent, upon irradiation, binds covalently to brain synaptosomes preparation and to gel-filtered platelets. Two-dimensional SDS-polyacrylamide gel electrophoresis-isoelectric focusing and tritium fluorography analysis indicate that two synaptosomal proteins and four platelets proteins were labelled by [3H]azidoimipramine.
D000345:Affinity Labels / Q000494:pharmacology*; D000818:Animals; D001386:Azides / Q000138:chemical synthesis / Q000494:pharmacology*; D001792:Blood Platelets / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D012701:Serotonin / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism*
D000345:Affinity Labels; D001386:Azides; D002352:Carrier Proteins; C025366:serotonin-binding protein; D012701:Serotonin; C033466:2-azidoimipramine