title: A third DNA polymerase from Spiroplasma citri and two other spiroplasmas.
abstract: Recently, two DNA polymerases (ScA and ScB) were isolated and characterized from Spiroplasma citri. We now have found a third DNA polymerase (ScC) not only in S. citri but also in the serologically related honeybee spiroplasma BC3 and the unrelated flower spiroplasma BNR1. Enzyme ScC is N-ethylmaleimide (NEM) sensitive. The three DNA polymerases from the honeybee spiroplasma seem to be similar to the respective enzymes of S. citri. However, whereas the NEM-resistant enzyme ScA from S. citri and that from the BC3 honeybee spiroplasma are retained on DEAE-cellulose and require 0.09 M KCl for elution, the NEM-resistant enzyme A from the flower spiroplasma BNR1 is not retained.
mesh_terms: D002848:Chromatography, DEAE-Cellulose; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification* / Q000378:metabolism; D005033:Ethylmaleimide / Q000494:pharmacology; D013149:Spiroplasma / Q000201:enzymology*
chemical_list: D004259:DNA-Directed DNA Polymerase; D005033:Ethylmaleimide