title: Structure of a modified nucleoside in archaebacterial tRNA which replaces ribosylthymine. 1-Methylpseudouridine.
abstract: The structure of a modified nucleoside isolated from Halococcus morrhuae tRNA has been established as 1-methylpseudouridine, based on mass spectrometry and gas chromatography carried out on 0.2 A260 unit of material. Not previously found in nucleic acids, 1-methylpseudouridine appears to be unique to archaebacterial tRNA, where it replaces ribosylthymine in the T psi C loop of most of the tRNAs examined thus far.
mesh_terms: D002849:Chromatography, Gas; D006088:Gram-Negative Aerobic Bacteria / Q000032:analysis*; D013058:Mass Spectrometry; D011560:Pseudouridine / Q000031:analogs & derivatives* / Q000032:analysis; D012343:RNA, Transfer / Q000302:isolation & purification*; D014529:Uridine / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: C013608:1-methylpseudouridine; D011560:Pseudouridine; C009182:ribothymidine; D012343:RNA, Transfer; D014529:Uridine