title: Developmental changes in the activities of prolinase and prolidase in rat salivary glands, and the effect of thyroxine administration.
abstract: As the salivary glands are interesting tissues to study proliferation, we studied the activities of prolinase and prolidase using Pro-Ala and Pro-Hyp as substrates, respectively, in developing rat salivary glands between day 1 and week 10 after birth. Developmental changes of prolinase activity in the submandibular and sublingual glands were similar to those in the parotid gland, which steadily increased and reached the adult level by 20-25 days after birth. However, the changes in the activity of prolidase in the submandibular and sublingual glands were different from those in the parotid gland: the activity in the parotid gland slowly increased with maturation and reached a maximum level on day 30, but the activity in the submandibular and sublingual glands continuously increased with maturation. When thyroxine was injected every two days from day 1 to day 19, both enzyme activities were induced precociously in the parotid gland but not in the submandibular and sublingual glands. On the study of regional distribution in rat tissues, the correlation coefficient between prolinase and prolidase activities was high in the peripheral but not high in the brain regions. These results indicate that the physiological roles of prolinase and prolidase are very similar but not the same.
mesh_terms: D000375:Aging; D000818:Animals; D001921:Brain / Q000201:enzymology; D004150:Dipeptidases / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D011392:Proline / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012469:Salivary Glands / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D013379:Substrate Specificity; D013974:Thyroxine / Q000494:pharmacology*; D014018:Tissue Distribution
chemical_list: D011392:Proline; D004150:Dipeptidases; C022454:prolyl dipeptidase; C022449:proline dipeptidase; D013974:Thyroxine