Determination of a glucose-containing tetrasaccharide in urine by radioimmunoassay.
A radioimmunoassay is described that allows rapid determination of a urinary oligosaccharide -- Glc alpha 1-6Glc alpha 1-4Glc alpha 1-4Glc [(Glc)4] -- at concentrations greater than 2 pmol/microliter. Antibodies produced in rabbits immunized with the phenethylamine derivative of (Glc)4 coupled to keyhole limpet hemocyanin bind tritiated (Glc)4-alditol. Studies comparing the activities of (Glc)4 and several of its derivatives and analogues as inhibitors of binding of tritiated (Glc)4-alditol show that (Glc)4 is detected with maximum specificity and sensitivity only after reduction to (Glc)4-alditol. Quantitation of (Glc)4 in urine samples reduced with sodium borohydride gives excellent agreement with a previously used by more laborious method that employs gas-liquid chromatography-mass spectrometry (GLC/MS) (correlation coefficient = 0.98). Studies of the excretion rate of (Glc)4 in urine of women during pregnancy using the radioimmunoassay method confirm and extend previous results using the GLC/MS method.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002417:Cattle; D005260:Female; D005947:Glucose; D006801:Humans; D009844:Oligosaccharides / Q000276:immunology / Q000652:urine*; D011247:Pregnancy; D011817:Rabbits; D011863:Radioimmunoassay; D013012:Sorbitol
D009844:Oligosaccharides; C033620:glucose tetrasaccharide; D013012:Sorbitol; D005947:Glucose