Liquid chromatography of codeine in plasma with fluorescence detection.
This analytical method for easier determination of codeine in human plasma is based on "high-performance" liquid chromatography for separation and the natural fluorescence of codeine for detection. Codeine is extracted from alkalinized plasma with a mixture of hexane and dichloromethane, and the extract is further purified and chromatographed. The method can be used for routine assay of codeine at the concentrations of 10 micrograms/L or greater in human plasma. As little as 4 micrograms/L can be detected. Coefficients of variation for the assay of codeine in the concentration range of 10 to 100 micrograms/L were 2.2-7.4% (n = 6). We used this method to establish a concentration/time profile for plasma from a human volunteer after a 60-mg oral dose of codeine sulfate.
D000284:Administration, Oral; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D003061:Codeine / Q000008:administration & dosage / Q000097:blood*; D005189:False Positive Reactions; D005453:Fluorescence; D006801:Humans; D012016:Reference Values; D013997:Time Factors
D003061:Codeine