Structure of O-glycosidically linked sugar units from plasma membranes of an ascites hepatoma, AH 66.
The structures of O-glycosidically linked oligosaccharides from plasma membranes of AH 66 ascites hepatoma cells have been determined. Glycopeptides were prepared from a Triton X-100 extract of the cells by pronase digestion. From the digest, the glycopeptide containing O-glycosidically linked oligosaccharides was isolated by gel filtration and electrophoresis on a cellulose acetate block. The oligosaccharide chains were isolated as reduced oligosaccharides by treating the glycopeptide with alkaline borohydride. Neutral and acidic oligosaccharides were separated on a column of Dowex 1, and then each of them was further fractionated by gel filtration on Sephadex G-25 (superfine), high voltage paper electrophoresis, and paper chromatography. One reduced monosaccharide, three neutral, and five acidic reduced oligosaccharides were isolated. On the basis of carbohydrate compositions, mass spectra of permethylated oligosaccharides, methylation analysis, and exoglycosidase digestion, their structures were deduced to be as follows: (formula, see text).
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002462:Cell Membrane / Q000032:analysis; D005260:Female; D006020:Glycopeptides / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D008114:Liver Neoplasms, Experimental / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis*; D009363:Neoplasm Proteins / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis*; D051381:Rats
D006020:Glycopeptides; D006027:Glycosides; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D009844:Oligosaccharides