Characterization of sister chromatid exchange induction by hydrogen peroxide.
Hydrogen peroxide (H2O2) induces SCEs in V-79 Chinese hamster cells. The incorporation of BrdUrd sensitizes the cells to the action of H2O2, but damage leading to the formation of SCEs can also be induced in DNA not substitued with BrdUrd. Catalase leads to a complete reduction of the SCE-producing as well as the toxic effect. The amino acid L-cysteine does not reduce the H2O2-induced SCE-frequencies. The results yield information about the relevance of SCE investigations in vitro for the determination of DNA-damaging substances and about the effects of antimutagenic SH-compounds.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002374:Catalase / Q000494:pharmacology; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D003434:Crossing Over, Genetic / Q000187:drug effects*; D003545:Cysteine / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D004357:Drug Synergism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D004247:DNA; D006861:Hydrogen Peroxide; D002374:Catalase; D001973:Bromodeoxyuridine; D003545:Cysteine