title: The effect of elevated potassium on the adult mollusc giant neurone survival and neurite formation in culture.
abstract: The number of viable neurones calculated as soon as adult mollusc ganglia tissue was digested and over different periods of cultivation, show that elevated K+ prevents degradation of intercellular contacts, preserves neurones against lysis, prolongs the time course of neurone survival in culture and inhibits neurite formation. K+ depolarizing effect is maintained throughout the whole course of cultivation; when elevated K+ is substituted by normal K+ the resting potential of neurones recovers. The effect of elevated K+ on neurone survival and the capability for neurite formation is not due to membrane depolarization, but seems to be related to an influence on intracellular ionic homeostasis.
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005724:Ganglia / Q000166:cytology*; D008974:Mollusca; D009410:Nerve Degeneration / Q000187:drug effects; D009416:Nerve Regeneration / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D011188:Potassium / Q000494:pharmacology*; D012964:Sodium / Q000494:pharmacology
chemical_list: D003470:Culture Media; D012964:Sodium; D011188:Potassium