title: The effect of chemical and physiological factors on the kinetics for product formation as it relates to enzyme activity and concentration, reaction time and to substrate adsorption and affinity.
abstract: 1. The dependency of product formation on reaction time and on enzyme concentration was studied with certain purified enzymes and liver microsomal mixed-function oxidase system. 2. The product-formation-time relation was estimated with different enzyme and/or substrate concentrations for the reactions limited by diffusion. 3. The kinetic constants of the product-time relations in these diffusion systems have been verified experimentally to give a more specific and detailed characterization of an enzyme system under a variety of physiologic and reaction conditions. 4. The influence of inhibitors and activators in vitro, the effect of enzyme preparation technic, aging, starvation, pretreatment with somatotropic hormone, phenobarbital or 3-methylcholanthrene, and the effect of a tumor were studied. 5. The influence of vitamin C deficiency in guinea pig was also studied.
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology; D000818:Animals; D001206:Ascorbic Acid Deficiency / Q000201:enzymology; D004789:Enzyme Activation; D004791:Enzyme Inhibitors; D004798:Enzymes / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D007700:Kinetics; D008297:Male; D008433:Mathematics; D008748:Methylcholanthrene / Q000494:pharmacology; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology*; D008956:Models, Chemical; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D013217:Starvation / Q000201:enzymology; D013329:Structure-Activity Relationship; D013997:Time Factors
chemical_list: D004791:Enzyme Inhibitors; D004798:Enzymes; D008748:Methylcholanthrene; D010634:Phenobarbital