Effect of haloperidol on the synthesis of DNA in the pituitary gland of the rat.
The administration of haloperidol increased serum prolactin and decreased the pituitary concentration of prolactin 15 min after its administration. Concomitantly there was a stimulation in the synthesis of DNA and the activity of DNA polymerase alpha in the anterior pituitary gland that was greater in oestrogenized than in non-oestrogenized male rats. Both these effects were greatly reduced by clomiphene in the oestrogenized male rats, although it did not affect the release of prolactin produced by haloperidol. In non-oestrogenized animals clomiphene abolished the stimulatory effect of haloperidol on the synthesis of DNA. These results suggest that the reduction in the intracellular levels of prolactin are a primary event in the oestrogen mediated stimulation of cell proliferation by prolactin releasing agents.
D000818:Animals; D002996:Clomiphene / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D004257:DNA Polymerase II / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology; D006220:Haloperidol / Q000494:pharmacology*; D008297:Male; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism; D011388:Prolactin / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D004967:Estrogens; D002996:Clomiphene; D011388:Prolactin; D004247:DNA; D004257:DNA Polymerase II; D004259:DNA-Directed DNA Polymerase; D006220:Haloperidol