A simplified method for the pentose phosphate pathway assay in red cells.
1. A simplified assay for the flux through the pentose phosphate and the Embden-Meyerhof pathways in the red cell is described. 2. [1-14C]glucose is metabolized to 14CO2 and 14C-anions. 3. 14CO2 is trapped with KOH and residual [14C]glucose and anions are separated by an enzymatic method, which is simpler and quicker than the usual chromatographic method.
D000838:Anions / Q000302:isolation & purification; D001786:Blood Glucose / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D010428:Pentosephosphates / Q000097:blood*
D000838:Anions; D001786:Blood Glucose; D010428:Pentosephosphates