Cyanine dye measurement of a light-induced transient membrane potential associated with the metarhodopsin II intermediate in rod-outer-segment membranes.
The fluorescent dye 3,3'-dipropylthiodicarbocyanin iodide [diS-C3-(5)] is used as a probe of fast membrane potential variations in the study of rod-outer-segment membranes. The formation of metarhodopsin II is found to be associated with the generation of a transmembrane potential, which is dissipitated by ionic movements across the membrane; its risetime is very similar to that of the protonation of the protein, which is also associated with the formation of metarhodopsin II.
D000818:Animals; D052160:Benzothiazoles; D002232:Carbocyanines; D002417:Cattle; D002462:Cell Membrane / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008027:Light; D008564:Membrane Potentials; D010786:Photoreceptor Cells / Q000502:physiology*; D011804:Quinolines; D012168:Retinal Pigments / Q000378:metabolism*; D012243:Rhodopsin / Q000031:analogs & derivatives / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D052160:Benzothiazoles; D002232:Carbocyanines; D011804:Quinolines; D012168:Retinal Pigments; C012944:3,3'-dipropyl-2,2'-thiadicarbocyanine; C013110:metarhodopsins; D012243:Rhodopsin