Affinity chromatography of viroid RNAs.
The application of a structure- and GC-specific affinity adsorbent for the purification of viroid RNAs is described. Cross-linked bisacrylamide gels to which the intercalating dye phenyl neutral red is covalently attached [2] constitute a powerful tool for the rapid, quantitative, and selective isolation of viroid as well as viroid complementary RNAs from crude, plant polysaccharides containing nucleic acid fractions.
D000609:Aminoacridines; D002846:Chromatography, Affinity; D004396:Coloring Agents; D009499:Neutral Red / Q000031:analogs & derivatives; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000302:isolation & purification*; D014772:Viroids / Q000737:chemistry*
D000609:Aminoacridines; D004396:Coloring Agents; D012367:RNA, Viral; C102822:phenyl neutral red; D009499:Neutral Red; C025902:acridine yellow