Partial purification and characterization of lenticular S-adenosylmethionine synthetase.
Lenticular S-adenosylmethionine synthetase has been partially purified and characterized. Lower levels of S-adenosylmethionine synthetase were detected by a sensitive radioactive assay procedure in the lenticular extract obtained from the pooled lenses of alloxan-treated rats 96 days of age as compared to the nontreated controls. With respect to chromatographic behavior and kinetic analysis, lenticular S-adenosylmethionine synthetase closely resembled that of the corresponding liver enzyme.
D000496:Alloxan / Q000494:pharmacology; D000818:Animals; D002386:Cataract / Q000201:enzymology*; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology; D007908:Lens, Crystalline / Q000187:drug effects / Q000201:enzymology*; D008716:Methionine Adenosyltransferase / Q000032:analysis* / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D014166:Transferases / Q000032:analysis*
D000496:Alloxan; D014166:Transferases; D008716:Methionine Adenosyltransferase