Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells.
DNA from T24, a cell line derived from a human bladder carcinoma, can induce the morphological transformation of NIH 3T3 cells. Using techniques of gene rescue to clone the gene responsible for this transformation, we have found that it is human in origin, less than 5 kilobase pairs in size and is homologous to a 1,100-base polyadenylated RNA species found in T24 and HeLa cells. Blot analysis indicates extensive restriction endonuclease polymorphism near this gene, in human DNAs.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D003001:Cloning, Molecular / Q000379:methods; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D005796:Genes; D008040:Genetic Linkage; D006801:Humans; D051379:Mice; D009693:Nucleic Acid Hybridization; D010920:Placenta / Q000502:physiology; D011110:Polymorphism, Genetic; D011247:Pregnancy; D014170:Transformation, Genetic; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm