Assay of bromhexine in human plasma by capillary gas--liquid chromatography with nitrogen-selective detection and selected ion monitoring.
A specific, sensitive method for the determination of bromhexine in human plasma is described. It comprises a selective extraction procedure and a specific determination with capillary gas--liquid chromatography and nitrogen-selective flame ionization detection. The detection limit of the assay is about 0.5 ng/ml. The specificity of the assay was checked by gas chromatography--mass spectrometry. The method is applied to the pharmacokinetics of bromhexine in humans.
D000328:Adult; D001964:Bromhexine / Q000008:administration & dosage / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007263:Infusions, Parenteral; D008297:Male
D001964:Bromhexine