The fate of antibiotics added to cell cultures used in virology.
The use of cell cultures has revolutionised virology over the past three decades. Antibiotics have played a key role in this revolution. However, it is curious that there appears to be very little published data on the fate of antibiotics added to cell cultures (as opposed to culture media). The experiments described in this paper were designed to show whether antibiotics added to cell cultures lost potency at a different rate from those added to cell culture media alone. In general, potency was lost more rapidly in the presence of cells but the differences were not such as to suggest enzymic breakdown of the antibiotics, and were only revealed by the more sensitive of the two assay methods used.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D006367:HeLa Cells; D006801:Humans; D013997:Time Factors; D014776:Virus Cultivation
D000900:Anti-Bacterial Agents; D003470:Culture Media