Cell surface carbohydrates in Crithidia deanei: influence of the endosymbiote.
The cell surface of the symbiote-containing and symbiote-free strains of Crithidia deanei were characterized comparatively by using 22 highly purified lectins with specificities for N-acetyl glucosamine, N-acetyl galactosamine, galactose, mannose-like residues, fucose and sialic acid. The specificity of the cell surface carbohydrate in both strains were analyzed by agglutination and lectin-binding assays. C. deanei with or without endosymbiote was specifically agglutinated by lectins from Triticum vulgaris (WGA) and Aaptos papillata suggesting the presence of D-GlcNAc residues. However, agglutination was stronger with the symbiote-free cells than with symbiote-containing organisms. The D-GalNAc-binding lectin from Wistaria floribunda also reacted most effectively with symbiote-free flagellates. Among D-Gal-binding lectins it was observed that those from Axinella polypoides and Ricinus communis I selectively agglutinated symbiote-free cells. In contrast the lectin from Arachys hypogaeae bound preferentially to the symbiote containing organisms. Both strains of C. deanei agglutinated strongly with the lectins concanavalin A and that from Lens culinaris (lectins for D-mannose-like residues). Conversely no cell agglutination occurred with the L-fucose-binding lectins Lotus tetragonolobus and Ulex europeus. The pattern of agglutination induced by the lectin from Limulus polyphemus of symbiote-free organisms was similar to that of symbiote-containing cells indicating the presence of sialic acid on the cell surface of both strains of C. deanei. These results indicate that the presence of the endosymbiote changes the lectin-binding sites at C. deanei surface membrane.
D002241:Carbohydrates / Q000032:analysis*; D003421:Crithidia / Q000032:analysis* / Q000502:physiology; D006017:Glycolipids / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D037102:Lectins; D013559:Symbiosis
D002241:Carbohydrates; D006017:Glycolipids; D006023:Glycoproteins; D037102:Lectins