Immunocytochemical demonstration of beta-lipotropin in mouse pituitary cells and neurons cultured in vitro.
Specific antiserum directed against human beta-lipotropin was used to demonstrate the presence of beta-lipotropin immunoreactivity in dissociated cell cultures of mouse pituitary, hypothalamus, spinal cord and dorsal root ganglia. A large population of pituitary cells and hypothalamic neurons were positive for beta-lipotropin immunoreactivity, while only 10-20% of the neurons from spinal cord and dorsal root ganglia were stained. The specificity of the beta-lipotropin immunoreaction was confirmed by blocking the reaction by prior absorption of the antiserum with added beta-lipotropin.
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D051379:Mice; D009420:Nervous System / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism; D008083:beta-Lipotropin / Q000378:metabolism*
D008083:beta-Lipotropin