Expression of adhesion-related membrane components in adherent versus nonadherent hamster melanoma cells.
The existence of integral membrane components that are involved in cell-substratum adhesion has been postulated. Using an immunochemical approach developed in this laboratory, we provide further evidence for the role in cell-substratum adhesion of integral membrane glycoproteins within a molecular weight region of 120,000-140,000. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of material enriched approximately 100-fold in adhesion-related components revealed the 120,000-140,000 Mr glycoproteins in an adherent hamster melanoma cell line. These glycoproteins are greatly reduced in a nonadherent variant. Induction of adhesion in these cells by exposure to BudR is accompanied by re-expression of the surface adhesion antigens.
D000818:Animals; D000906:Antibodies; D002448:Cell Adhesion; D002460:Cell Line; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism*; D008545:Melanoma / Q000378:metabolism*; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000276:immunology / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000378:metabolism; D009500:Neutralization Tests
D000906:Antibodies; D006023:Glycoproteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins