title: Dissecting the hematopoietic microenvironment. IV: regeneration of splenic microstructure- prerequisites and chronology of reconstruction.
abstract: A chronologic study was made of whole organ necrosis and subsequent regeneration in the pedicle ligated mouse spleen. The whole organ necrosis involved all but a thin layer of cells including and beneath the splenic capsule. Cellular regeneration issued from these surviving cells and was seen to proceed inward along remaining noncellular reticular fiber tracts throughout the spleen, resulting in complete architectural restoration. Destruction of these fibers resulted in failure of orderly reconstruction of splenic substance. A subcapsular vascular space was made prominent by the ligation and appeared to completely circumscribe the internal splenic substance. Regeneration appeared to begin with cells just central to this space. Reconstruction of the ligated spleen was architecturally complete within 40 days.
mesh_terms: D000375:Aging; D000818:Animals; D001808:Blood Vessels / Q000473:pathology / Q000502:physiology; D003238:Connective Tissue / Q000473:pathology / Q000502:physiology; D005260:Female; D005347:Fibroblasts / Q000473:pathology / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000473:pathology / Q000502:physiology*; D008026:Ligation; D008214:Lymphocytes / Q000473:pathology / Q000502:physiology; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D009336:Necrosis; D012038:Regeneration; D013154:Spleen / Q000098:blood supply / Q000473:pathology / Q000502:physiology*; D000267:Tissue Adhesions