Isolation and characterization of alkali-labile oligosaccharide units from porcine erythrocyte glycophorin.
The oligosaccharide units of glycophorin isolated from porcine erythrocyte membranes were released by alkaline borohydride treatment and purified by gel filtration and ion-exchange chromatography. Structures of the O-glycosidic oligosaccharides were determined by methylation analysis, the methylated sugar being identified by gas-liquid chromatography-mass spectrometry and nitrous acid deamination after hydrazinolysis. The major oligosaccharide was a trisaccharide, Gal(1 leads to 3)[Neu-NGly(2 leads to 6)]GalNAc. The other oligosaccharides were larger and contained Glc-NAc. One was a pentasaccharide, Gal(1 leads to 3)Gal(1 leads to 4)GlcNAc(1 leads to 3)Gal(1 leads to 3)GalNAc. The structure of the trisaccharide was also analyzed by direct-probe mass spectrometry of the permethylated derivative, and the result obtained was consistent with the proposed structure.
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002918:Chymotrypsin; D004910:Erythrocyte Membrane / Q000032:analysis; D006020:Glycopeptides / Q000032:analysis; D006021:Glycophorins; D013058:Mass Spectrometry; D008745:Methylation; D009844:Oligosaccharides / Q000302:isolation & purification*; D012795:Sialoglycoproteins; D013552:Swine
D006020:Glycopeptides; D006021:Glycophorins; D009844:Oligosaccharides; D012795:Sialoglycoproteins; D002918:Chymotrypsin