Development of a monoclonal antibody against a tumor-associated antigen.
A monoclonal antibody-producing hybrid cell line was obtained by fusing mouse myeloma cells with spleen cells from a mouse immunized with C6 glioma cells. This antibody binds to a specific cell-surface antigen that is present on C6 rat glioma cells, transformed astrocytes and oligodendrocytes, and a human glioma cell line but is absent on a normal glial cell line, fibroblasts, and primary cultures of astrocytes and oligodendrocytes. The antigen also appears on tumor tissue of transformed oligodendrocytes but not on normal brain tissue.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D002471:Cell Transformation, Neoplastic; D005910:Glioma / Q000276:immunology*; D006801:Humans; D009457:Neuroglia / Q000276:immunology; D051381:Rats
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm