Ecdysteroid induction of cell surface contacts in a Drosophila cell line.
Drosophila Kc cells are usually spherical in shape and are relatively free of cell-cell contacts. Soon after exposure to 20-hydroxyecdysone they develop extensions, assume a fibroblastic shape, and closely adhere to adjacent cells by means of cell-cell contacts to form large multicellular aggregates. This adhesive nature is enhanced with trypsin treatment without disruption of cell shape. Simultaneous addition of 20-hydroxyecdysone and juvenile hormone results in diminished aggregate formation without preventing the changes in cell shape. These factors point to the possibility that 20-hydroxyecdysone stimulates modifications in the cell surface that may be important during embryological differentiation. Key words Kc cells, aggregations, recognition, juvenile hormone.
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects*; D002460:Cell Line; D004330:Drosophila; D004441:Ecdysterone / Q000494:pharmacology*; D007605:Juvenile Hormones / Q000494:pharmacology; D014357:Trypsin / Q000378:metabolism
D007605:Juvenile Hormones; D004441:Ecdysterone; D014357:Trypsin