High performance liquid chromatographic determination and clearance time of aflatoxin residues in swine tissues.
Liver, kidney, and muscle tissues from swine given a single oral dose of 1.2 mg aflatoxins/kg body weight and from young pigs fed an aflatoxin-contaminated diet (0,400, or 800 ng/g feed) for 10 weeks were analyzed for aflatoxin residues. Extraction was performed according to the method of Stubblefield and Shotwell. Aflatoxin residues were detected by high performance liquid chromatography (HPLC), and residue levels were correlated with withdrawal times. Residues were present in liver and kidney but not muscle by 12 h post-dosing; residues were detected in muscle by after 24 h. Residue levels decreased over the 72 h period in all tissues. No aflatoxin residues were detected in the muscle from any pig 72 h post-dosing or in those pigs fed the 400 ng/g diet, but were present in the muscle of pigs fed the 800 ng/g diet.
D000348:Aflatoxins / Q000008:administration & dosage / Q000378:metabolism*; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001822:Body Burden; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D013552:Swine; D013997:Time Factors
D000348:Aflatoxins