Kinetics of the aromatic L-amino acid oxidase from dead bovine spermatozoa and the effect of catalase on fertility of diluted bovine semen stored at 5 degrees C and ambient temperatures.
At limiting velocity 50 x 10(6) dead spermatozoa consumed 13.6 microliters O2/h and degraded 6.1 x 10(-7) mol  L-phenylalanine/h. Substrate concentration in egg yolk was equivalent to 6.1 x 10(-3) M-L-phenylalanine. At a substrate concentration of 3 x 10(-2) M-L-phenylalanine, oxidase activity was significantly affected by oxygen tension (r = 0.947, P less than 0.01) and temperature (r = 0.995, P less than 0.01). Non-return rates were not affected when semen was stored at 5 degrees C in 20% egg yolk diluents with catalase but were significantly increased with semen stored at ambient temperatures of 15-23 degrees C.
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D002374:Catalase / Q000494:pharmacology*; D002417:Cattle; D002470:Cell Survival; D005298:Fertility / Q000187:drug effects*; D007700:Kinetics; D050830:L-Amino Acid Oxidase; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D010649:Phenylalanine / Q000378:metabolism; D012662:Semen Preservation; D013094:Spermatozoa / Q000201:enzymology* / Q000378:metabolism; D013696:Temperature
D010649:Phenylalanine; D002374:Catalase; D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase