Ouabain-sensitive 86Rb(K) influx is linked to transepithelial Na transport in pig kidney cell line.
The pig kidney cell line, LLC-PK1, exhibits rheogenic D-glucose coupled transepithelial Na+ transport that is inhibited by phlorizin. By measuring the difference in initial rates of influx of 86Rb+ with and without coupled Na+ transport, we can demonstrate an 86Rb+ uptake linked to Na+ transport, The simultaneous determination of phlorizin-inhibited Na coupled D-[3H] glucose uptake and 86Rb+ influx allows calculation of an Na+/Rb+ stoichiometry that is consistent with an electrogenic Na+ for Rb+ exchange.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002460:Cell Line; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D010042:Ouabain / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism*; D012413:Rubidium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D013552:Swine
D010042:Ouabain; D012964:Sodium; D012413:Rubidium; D011188:Potassium