The uptake of etiocholanolone by human bone marrow immature granulocytes.
A cell fraction enriched in immature granulocytes was obtained after fractionation of human bone marrow by a three-step procedure. This fraction sediments at an average density of 1.073 and has a high content of myelocytes and metamyelocytes. It showed the highest specific uptake of etiocholanolone when compared to other bone marrow cell fractions. Autoradiographic studies demonstrated a higher number of silver grains over myelocytes and metamyelocytes than in other cell types. These results suggest the presence in human bone marrow of a target cell for etiocholanolone, located in the late mitotic or postmitotic pool or granulocytes.
D001345:Autoradiography; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D005043:Etiocholanolone / Q000378:metabolism* / Q000494:pharmacology; D006098:Granulocytes / Q000166:cytology / Q000378:metabolism*; D006410:Hematopoiesis / Q000187:drug effects; D006801:Humans; D007958:Leukocyte Count
D005043:Etiocholanolone