Influence of triglyceride-rich lipoproteins on nephelometry of apolipoprotein A-I.
Measurement of apolipoprotein A-I in native human sera by endpoint or rate nephelometry may give falsely high results for sera that have a high triglyceride content. Such erroneous determinations of apolipoprotein A-I can be avoided if one instead assays supernates of sera from which apolipoprotein B-containing particles have been precipitated with phosphotungstic acid and MgCl2. A high triglyceride concentration in serum does not impair the completeness of the precipitation of apolipoprotein B-containing lipoproteins or the analytical recovery of apolipoprotein A-I. In a prospective epidemiologic study, by using rate nephelometry we found that there were different mean concentrations for apolipoprotein A-I in supernates of sera from men (1.36 g/L) and women (1.49 g/L). Furthermore, the known risk factors for atherosclerosis showed different correlations with apolipoprotein A-I than with high-density lipoprotein cholesterol.
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D001053:Apolipoproteins / Q000097:blood*; D001054:Apolipoproteins A; D001055:Apolipoproteins B; D001161:Arteriosclerosis / Q000209:etiology*; D002784:Cholesterol / Q000097:blood*; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D011446:Prospective Studies; D012016:Reference Values; D012306:Risk; D012737:Sex Factors; D012907:Smoking; D014280:Triglycerides / Q000097:blood
D001053:Apolipoproteins; D001054:Apolipoproteins A; D001055:Apolipoproteins B; D008075:Lipoproteins, HDL; D014280:Triglycerides; D002784:Cholesterol