Evaluation of different technical approaches for the research of human anti-Ia alloantisera.
Several strategies have been proposed for the research of human anti-Ia alloantisera. The present work evaluates the results of a screening made on multiparous sera, using either platelet-absorbed sera or unabsorbed sera against cells coated by anti-human-beta-2-microglobulin antibodies. When the results of the two approaches were compared, it was observed that the latter shows a higher frequency of positive reactions, being able to detect weak anti-Ia, and possibly non-HLA antibodies, which are not revealed with the usual test on platelet-absorbed serum. A significant correlation has been noted between the presence of anti-HLA-A,B,C and of anti-Ia, demonstrating that the best source of Ia antibodies from multiparous women are sera also containing anti-HLA-A,B,C.
D001788:Blood Grouping and Crossmatching; D001792:Blood Platelets; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D000949:Histocompatibility Antigens Class II; D006801:Humans; D007518:Isoantibodies / Q000032:analysis*; D011247:Pregnancy; D001613:beta 2-Microglobulin
D000949:Histocompatibility Antigens Class II; D007518:Isoantibodies; D001613:beta 2-Microglobulin