Primary culture of prostatic region entoderm of human fetal sinus urogenitalis: morphological, histochemical, and electrophoretic characteristics.
Primary cultures of entoderm from the prostatic region of the sinus urogenitalis obtained from 9 to 12-week-old human fetuses were established by explantation of presumptive prostate tissue on collagen. The predominant growth of entodermal cells made it possible to obtain cultures, without use of enzymes or EDTA, which were practically free of mesenchyme. Histochemical and electrophoretic methods demonstrated certain properties of the parent presumptive prostatic entodermal cells in the cultured cells. Karyological alterations observed during the course of cultivation are described.
D000135:Acid Phosphatase / Q000032:analysis; D002453:Cell Cycle; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D004586:Electrophoresis; D004622:Embryo, Mammalian / Q000166:cytology; D004707:Endoderm / Q000032:analysis / Q000166:cytology*; D005260:Female; D005333:Fetus / Q000166:cytology*; D006801:Humans; D007620:Karyometry; D007621:Karyotyping; D008297:Male; D011467:Prostate / Q000032:analysis / Q000166:cytology*
D000135:Acid Phosphatase