Secretion of newly taken-up ascorbic acid by adrenomedullary chromaffin cells.
Primary cultures of bovine adrenomedullary cells accumulate carbon-14-labeled ascorbic acid through a saturable and energy-dependent process. The newly taken-up ascorbate is released concomitantly with catecholamines upon stimulation of chromaffin cell secretion. The release of ascorbate is Ca2+-dependent and mediated through activation of nicotinic receptors. These results indicate that exogenous ascorbate taken up into chromaffin cells is incorporated in situ into a secretable compartment, probably the catecholamine-containing chromaffin vesicles.
D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D001693:Biological Transport, Active; D002118:Calcium / Q000502:physiology; D002417:Cattle; D002478:Cells, Cultured; D002837:Chromaffin Granules / Q000378:metabolism; D002838:Chromaffin System / Q000378:metabolism*; D009538:Nicotine / Q000494:pharmacology; D011978:Receptors, Nicotinic / Q000502:physiology; D012636:Secretory Rate / Q000187:drug effects
D011978:Receptors, Nicotinic; D009538:Nicotine; D001205:Ascorbic Acid; D002118:Calcium