Kinetics of glucuronidation of phenol-derivatives of benzo(a)pyrene with rat and mouse liver microsomes.
The kinetics of glucuronidation with 3- and 9-hydroxybenzo(a)pyrene (3-OH BP and 9-OH BP) were studied using rat and mouse liver microsomes. The UDP-glucuronyl transferase had two apparent Km values for 3- and 9-OH BP in both species, suggesting that the hepatic enzyme is efficient in glucuronidation of first-phase metabolites, especially at low concentrations of the carcinogen.
D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism*; D005965:Glucuronates / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008862:Microsomes, Liver / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D001580:Benzopyrenes; D005965:Glucuronates; D010636:Phenols