title: Curing effect of clorobiocin on Escherichia coli plasmids.
abstract: Clorobiocin, an inhibitor of the gyrB subunit of DNA gyrase, was used for the curing of some Escherichia coli plasmids. Of the plasmids studied, ampicillin resistant R28K and a miniplasmid derived from R1drd-19 were effectively eliminated. We also succeeded in eliminating the ColA factor from E. coli strain B834(pBS103), which was resistant to the effect of currently used curing agents. Although a derivative of ColE1-pBR322 was effectively cured by clorobiocin, the ColE1 plasmid was resistant to its effect. The ColV plasmid determining virulence was effectively eliminated.
mesh_terms: D000667:Ampicillin / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D009675:Novobiocin / Q000031:analogs & derivatives* / Q000494:pharmacology; D010403:Penicillin Resistance; D010957:Plasmids / Q000187:drug effects*; D013045:Species Specificity
chemical_list: D009675:Novobiocin; C006260:clorobiocin; D000667:Ampicillin