Ferrisiderophore reductase activity in Agrobacterium tumefaciens.
Reduction of the iron in ferriagrobactin by the cytoplasmic fraction of Agrobacterium tumefaciens strictly required NaDH as the reductant. Addition of flavin mononucleotide and anaerobic conditions were necessary for the reaction; when added with flavin mononucleotide, magnesium was stimulatory. This ferrisiderophore reductase activity may be a part of the iron assimilation process in A. tumefaciens.
D000693:Anaerobiosis; D002396:Catechols / Q000378:metabolism; D005486:Flavin Mononucleotide / Q000494:pharmacology; D007502:Iron Chelating Agents; D008274:Magnesium / Q000494:pharmacology; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D012231:Rhizobium / Q000201:enzymology*
D002396:Catechols; D007502:Iron Chelating Agents; D009243:NAD; C018813:agrobactin; D005486:Flavin Mononucleotide; D010088:Oxidoreductases; D009247:NADH, NADPH Oxidoreductases; C024253:ferrisiderophore reductase; D008274:Magnesium