Isolation and characterization of fibronectin from bovine aqueous humor.
Aqueous humor collected from freshly enucleated bovine eyes was passed over a gelatin affinity column, and bound material was eluted with 4M urea. When fractionated by sodium dodecyl sulfate electrophoresis, the eluted material co-migrated with human plasma fibronectin and protein standards of approximately 220,000 daltons. Amino acid analyses demonstrated a composition similar to that of human plasma fibronectin and fibronectin synthesized by cultured cells. The concentration of fibronectin in aqueous humor is approximately 100 times less than in human plasma.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001082:Aqueous Humor / Q000032:analysis*; D002417:Cattle; D002850:Chromatography, Gel; D005353:Fibronectins / Q000032:analysis / Q000097:blood / Q000302:isolation & purification*
D000596:Amino Acids; D005353:Fibronectins