title: Release of enzymes from cultured fibroblasts induced by lysophosphatidylcholine.
abstract: Release of enzymes from cultured fibroblasts was induced by exposure to the action of lysophosphatidylcholine (LPC). The effect of LPC was dependent on cell density. At identical densities a linear relationship between the concentration of LPC and the efflux of enzymes was found. Efflux of lactate dehydrogenase occurred at LPC concentrations of 5-10 micrograms/ml. Leakage of aspartate aminotransferase took place at higher concentrations, 10-25 micrograms/ml. Efflux of glutamate dehydrogenase was observed only at high concentrations of LPC, approximately 50 micrograms/ml. The efflux of the two former enzymes decreased with time.
mesh_terms: D001219:Aspartate Aminotransferases / Q000378:metabolism; D002478:Cells, Cultured; D004798:Enzymes / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008244:Lysophosphatidylcholines / Q000494:pharmacology*
chemical_list: D004798:Enzymes; D008244:Lysophosphatidylcholines; D007770:L-Lactate Dehydrogenase; D005969:Glutamate Dehydrogenase; D001219:Aspartate Aminotransferases