Anti-idiotype as antibody against the formyl peptide chemotaxis receptor of the neutrophil.
Anti-idiotypic antibodies have been produced in mice, guinea pigs, and goat against rabbit antibodies to the chemoattractant f Met-Leu-Phe. The anti-idiotypic antibodies were demonstrated by their capacity in the presence of preimmune rabbit serum to block the binding of 125I anti-f Met-Leu-Phe to f Met-Leu-Phe-human transferrin absorbed to microtiter plates. Goat anti-idiotypic antibodies were further demonstrated by their ability, when absorbed to Staphylococcus aureus, to selectively bind 125I anti-f Met-Leu-Phe. In addition, some of the goat anti-idiotypic IgG was able to bind nearly all rabbit and rat anti-f Met-Leu-Phe antibodies examined. This was shown by 1) the ability of antibodies produced in rabbits immunized with either f Met-Leu-Phe conjugated to goat IgG or f Met-Leu-Phe conjugated to keyhole limpet hemocyanin (KLH) when adsorbed to S. aureus to bind the 125I F(ab')2 goat anti-idiotype, and 2) the ability of various rat anti-f Met-Leu-Phe antibodies to block the binding of 125I rabbit anti-f Met-Leu-Phe to goat anti-idiotype absorbed to S. aureus. Finally, goat anti-idiotypic antibodies can also cross-react with the receptor on the rabbit neutrophil for the formyl peptides as evidenced by 1) the direct binding of goat anti-idiotypic IgG to the PMN, 2) the ability of F(ab')2 fragments to goat anti-idiotype to partially inhibit the binding of f Met-Leu-(3H)Phe to rabbit PMN, and 3) loss of anti-idiotype and anti-PMN receptor activity after passage over an anti-f Met-Leu-Phe column. These data support Jerne's hypothesis of "internal image" and suggest a feasible experimental approach for producing anti-cell receptor antibody without purifying the receptor.
D000818:Animals; D001667:Binding, Competitive; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D002846:Chromatography, Affinity; D006041:Goats; D006168:Guinea Pigs; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008715:Methionine / Q000031:analogs & derivatives*; D051379:Mice; D009239:N-Formylmethionine / Q000031:analogs & derivatives* / Q000276:immunology / Q000378:metabolism; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000276:immunology; D009842:Oligopeptides / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011971:Receptors, Immunologic; D013205:Staphylococcal Protein A / Q000378:metabolism; D013211:Staphylococcus aureus / Q000276:immunology; D014168:Transferrin / Q000378:metabolism
D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D009842:Oligopeptides; D011971:Receptors, Immunologic; D013205:Staphylococcal Protein A; D014168:Transferrin; D009239:N-Formylmethionine; D009240:N-Formylmethionine Leucyl-Phenylalanine; D008715:Methionine