Autoradiographic comparison of 96Tc-pyrophosphate and 45Ca bone uptake.
To compare the skeletal distribution of calcium and pyrophosphate complexes, autoradiograms of the growing zone of 10-week-old rabbit femurs were performed. Due to its stability to chemical and enzymatic hydrolysis and to the relatively long half-life of the isotope, 96Tc-labelled pyrophosphate was used. Calcium uptake was studied with carrier free 45Ca. For both tracers, the blood supply plays a significant role by delivering the radiopharmaceuticals. In a second step their partition was governed by mixing in different bone structures: calcium was incorporated inside the bone trabeculae and the mineral mass whereas labelled pyrophosphate remained in linear patterns surrounding bone spicules.
D000818:Animals; D001345:Autoradiography; D002118:Calcium / Q000378:metabolism*; D002122:Calcium Chloride / Q000008:administration & dosage; D002132:Calcium Radioisotopes; D005269:Femur / Q000378:metabolism*; D007275:Injections, Intravenous; D011122:Polyphosphates / Q000378:metabolism*; D011817:Rabbits; D013667:Technetium / Q000008:administration & dosage / Q000378:metabolism*; D016698:Technetium Tc 99m Pyrophosphate; D014003:Tin Polyphosphates / Q000008:administration & dosage / Q000378:metabolism*
D002132:Calcium Radioisotopes; D011122:Polyphosphates; D014003:Tin Polyphosphates; C017742:technetium Tc 99m stannous pyrophosphate; D016698:Technetium Tc 99m Pyrophosphate; D013667:Technetium; D002122:Calcium Chloride; D002118:Calcium