title: TOL plasmid pWW0 in constructed halobenzoate-degrading Pseudomonas strains: prevention of meta pathway.
abstract: The hybrid pathway for chlorobenzoate metabolism was studied in WR211 and WR216, which were derived from Pseudomonas sp. B13 by acquisition of TOL plasmid pWW0 from Pseudomonas putida mt-2. Chlorobenzoates are utilized readily by these strains when meta cleavage of chlorocatechols is suppressed. When WR211 utilizes 3-chlorobenzoate (3CB), the expression of catechol 2,3-dioxygenase (C23O) and the catabolic activities for chloroaromatics via the ortho pathway coexist as a consequence of inactivation of the meta cleavage activity by 3-chlorocatechol. Utilization of 4-chlorobenzoate (4CB) by WR216 presupposes the suppression of C23O by a spontaneous mutation in the structural gene, so that 4-chlorocatechol is not misrouted into the meta pathway. Such C23O- mutants were also selected when WR211 was grown continuously on 3CB. Our data explain why the phenotypic characters 3CB+ and Mtol+ (m-toluate) are compatible, whereas 4CB+ and Mtol+ are incompatible.
mesh_terms: D001565:Benzoates / Q000378:metabolism*; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase; D002396:Catechols / Q000378:metabolism; D002723:Chlorobenzoates / Q000378:metabolism*; D049308:Dioxygenases; D004790:Enzyme Induction; D010105:Oxygenases / Q000378:metabolism; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics*
chemical_list: D001565:Benzoates; D002396:Catechols; D002723:Chlorobenzoates; C031136:3-methylcatechol; C031137:3-chlorocatechol; C009503:3-toluic acid; D010105:Oxygenases; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase