[Ultrastructural changes in myocardial anoxia and reoxygenation].
The aim of this study is to describe the ultrastructural changes in anoxic rat hearts perfused with Langendorff technique and the effects of reoxygenation on myocardial cells. After 1 h of aerobic perfusion, the hearts were subjected to 30 min of anoxia and then to 5 and 30 min of reoxygenation. The following findings were observed: after 30 min of anoxia, loss of mitochondrial matrix and dilatation of the intracristal spaces. After 5 min of reoxygenation: diffuse mitochondrial swelling, sarcomeres disarranged and out of register, several contraction bands. After 30 min: no evidence of cytoplasmic or mitochondrial swelling. The maximum enzyme release was observed when a wide myofibrillar contraction occurred. These results may suggest that the oxygen availability consequent to myocardium anoxia lead to further cell damage.
D000818:Animals; D000860:Hypoxia / Q000473:pathology*; D008297:Male; D008933:Mitochondrial Swelling; D009200:Myocardial Contraction; D009206:Myocardium / Q000648:ultrastructure*; D010100:Oxygen; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D010100:Oxygen
Modificazioni ultrastrutturali in corso di anossia miocardica e riossigenazione.