Effects of 12-O-tetradecanoylphorbol-13-acetate and mezerein on gamma radiation-induced DNA repair in resting bovine lymphocytes.
The present study was undertaken to determine the effect of the potent tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA) on gamma radiation-induced DNA repair in resting (Go) lymphocytes. Mezerein, a non-promoter but a co-mitogen in lymphocytes, was used as a control agent. It was previously proposed that a possible mechanism for the action of tumor promoters was through the inhibition of DNA repair processes. Our results indicate that TPA does not inhibit DNA repair following gamma irradiation of Go lymphocytes. These data support the hypothesis that the tumor promoting ability of TPA is not a result of impaired repair of potentially mutagenic lesions in DNA.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000235:genetics; D004260:DNA Repair / Q000187:drug effects*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004224:Diterpenes; D005720:Gamma Rays; D007399:Interphase; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects*; D009369:Neoplasms / Q000209:etiology; D010703:Phorbol Esters / Q000494:pharmacology*; D010704:Phorbols / Q000494:pharmacology*; D013729:Terpenes; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D004224:Diterpenes; D010703:Phorbol Esters; D010704:Phorbols; D013729:Terpenes; C011309:mezerein; D004247:DNA; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide