A candidate reference method for uric acid in serum. I. Optimization and evaluation.
We describe our optimization and evaluation of a candidate Reference Method for uric acid in serum. Reaction parameters were optimized for a manual, enzymic method for uric acid in which highly purified microbial uricase is used to quantitate uric acid by a differential ultraviolet procedure. We evaluated the method in terms of freedom from interferences, analytical recovery, precision, and comparison with five other uric acid methods. We conclude that (a) the candidate uric acid Reference Method exhibits the least interference; (b) all six methods exhibit satisfactory analytical recoveries and precision; and (c) results by all six methods agree well. As a result of this evaluation study, the manual ultraviolet uricase method for uric acid, with Tris as buffer, was chosen as the candidate Reference Method for uric acid.
D000328:Adult; D000368:Aged; D001322:Autoanalysis; D005069:Evaluation Studies as Topic; D006801:Humans; D008297:Male; D008875:Middle Aged; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet; D014503:Urate Oxidase; D014527:Uric Acid / Q000097:blood*
D014527:Uric Acid; D014503:Urate Oxidase