Cleavage and cross-linking of proteins with osmium (VII) reagents.
The specific cleavage of proteins following reaction with osmium tetroxide is slowed down by tertiary amines which are known to be good ligands for osmium. Bidentate ligands are more effective than monodentate ligands. These same ligands also promote an intermolecular cross-linking reaction.
D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D008024:Ligands; D009992:Osmium; D010047:Ovalbumin; D011506:Proteins
D003432:Cross-Linking Reagents; D008024:Ligands; D011506:Proteins; D009992:Osmium; D010047:Ovalbumin