Hydrolysis of nerve gas by squid-type diisopropyl phosphorofluoridate hydrolyzing enzyme on agarose resin.
An enzyme purified from squid nerve that hydrolyzes the cholinesterase inhibitor diisopropyl phosphorofluoridate (DFP) has now been coupled to agarose beads. A column of this agarose-DFPase hydrolyzes the nerve gas 1,2,2-trimethylpropyl methylphosphonofluoridate (Soman). Although the more inhibitory of the four diastereoisomers of Soman are hydrolyzed least rapidly, a column of sufficient length will accomplish 95 percent hydrolysis whether measured by fluoride release or loss of cholinesterase-inhibiting power. The results suggest a means for detoxifying unwanted chemical warfare agents.
D000818:Animals; D049832:Decapodiformes / Q000201:enzymology*; D004800:Enzymes, Immobilized; D007531:Isoflurophate / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D009943:Organophosphorus Compounds / Q000378:metabolism*; D012999:Soman / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity
D004800:Enzymes, Immobilized; D009943:Organophosphorus Compounds; D007531:Isoflurophate; D012999:Soman