Proliferative capacity of murine hematopoietic stem cells in vitro.
Large numbers of granulocytes can be collected repeatedly from the supernatant medium of long-term cultures of mouse bone marrow cells. A constant relationship was found between the number of adherent hematopoietic stem cells and the lifetime cell production per culture. The data indicate that there is a limit to the proliferative capacity of normal and of irradiated stem cells. A similar limitation was found in the production of marked granulocytes from clonal cultures of "beige" C57 (bg/bgJ) stem cells placed in limiting dilutions into stromal culture layers.
D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division / Q000528:radiation effects; D002478:Cells, Cultured; D006098:Granulocytes / Q000502:physiology; D006410:Hematopoiesis / Q000528:radiation effects; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D051379:Mice; D013154:Spleen / Q000166:cytology