Intrinsic fluorescence of some myxo- and paramyxoviruses and of their subviral fractions.
Fluorescence spectra of Sendai and influenza A(H1N1) viruses have different emission maxima; their quantum yield is much lower than that of tobacco mosaic virus. Solubilized envelopes and nucleoproteins of Sendai, influenza and mumps virus have different half-bandwidth and relative quantum yield values of emission, according to the agent used for disruption. Thus emission (as well as excitation and absorption) spectra of Triton X-100-solubilized envelopes show a marked hypsochromic shift as compared with the envelopes obtained by Tween20-disruption. The results are correlated with the different disruption extent achieved with the two agents.
D000596:Amino Acids / Q000032:analysis; D002213:Capsid / Q000032:analysis; D003902:Detergents; D009109:Mumps virus / Q000648:ultrastructure; D009698:Nucleoproteins / Q000032:analysis*; D009975:Orthomyxoviridae / Q000032:analysis* / Q000648:ultrastructure*; D010222:Parainfluenza Virus 1, Human / Q000648:ultrastructure; D010252:Paramyxoviridae / Q000032:analysis* / Q000648:ultrastructure*; D013050:Spectrometry, Fluorescence; D013052:Spectrometry, X-Ray Emission; D014764:Viral Proteins / Q000032:analysis
D000596:Amino Acids; D003902:Detergents; D009698:Nucleoproteins; D014764:Viral Proteins