title: Hepatobiliary clearance of IgA immune complexes formed in the circulation.
abstract: The formation and clearance of circulating IgA immune complexes from blood to bile was investigated in this study. The i.v. injection of either MOPC-315, an IgA M-component with antidinitrophenyl (DNP) specificity, or TEPC-15, an IgA M-component of a different specificity, was followed by i.v. injection of 125I-DNP10-bovine serum albumin (BSA) as the antigen. The formation and clearance of IgA immune complexes in the circulation of MOPC-315-treated, but not TEPC-15-treated animals was demonstrated by immunoprecipitation with polyacrylamide beads coated with rabbit anti-mouse IgA. IgA-125I-DNP10-BSA complexes were identified in the bile from MOPC-315-treated, but not TEPC-15-treated animals utilizing this same immunoprecipitation technique. These observations suggest that the liver or bile ducts transport IgA immune complexes from blood into bile. The clearance of 125I-DNP10-BSA from the circulation was documented by coprecipitation with rabbit anti-BSA and BSA. The clearance of this circulating antigen was slower in the MOPC-315-treated than in the TEPC-15-treated animals suggesting that under the conditions of the present experiment, circulating antigen is cleared more slowly after IgA immune complex formation.
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism*; D001202:Ascitic Fluid / Q000276:immunology; D001646:Bile / Q000276:immunology*; D004140:Dinitrophenols; D007070:Immunoglobulin A / Q000378:metabolism*; D008099:Liver / Q000276:immunology*; D008297:Male; D009374:Neoplasms, Experimental / Q000276:immunology; D010954:Plasmacytoma / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000378:metabolism; D012710:Serum Albumin, Bovine
chemical_list: D000936:Antigen-Antibody Complex; D004140:Dinitrophenols; D007070:Immunoglobulin A; D012709:Serum Albumin; C034638:dinitrophenyl-bovine serum albumin; D012710:Serum Albumin, Bovine