The enucleated arterially perfused cat eye. I. Effects on protein permeability.
Protein permeability of the ciliary epithelium of enucleated arterially perfused cat eyes was studied. Tracer concentrations of 125I-albumin, in an arterial perfusate solution consisting of a balanced salt solution (BSS) without added colloid, was found in the anterior chamber fluid (AH) in a percentage similar to the AH/plasma ratio found for protein in the living cat eye. Stimulation of AH formation by acetylcholine plus eserine increased protein permeability markedly and by a similar amount in eyes perfused either with BSS or with 100% serum. These data indicate that arterial perfusion of the enucleated eye with BSS alone induces no gross abnormality of the blood-aqueous barrier.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001082:Aqueous Humor / Q000502:physiology*; D001798:Blood Proteins / Q000032:analysis; D002415:Cats; D002924:Ciliary Body / Q000502:physiology*; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D010477:Perfusion; D010539:Permeability; D010830:Physostigmine / Q000494:pharmacology; D011506:Proteins / Q000502:physiology*; D012965:Sodium Chloride
D001798:Blood Proteins; D011506:Proteins; D012965:Sodium Chloride; D010830:Physostigmine; D000109:Acetylcholine