title: Subcellular distribution of particle-associated enzymes in horse neutrophil leukocytes.
abstract: The subcellular components of purified neutrophil leukocytes from horse blood were fractionated by isopyknic equilibration in sucrose and metrizamide gradients. Five classes of particles have been identified: dense azurophil granules containing the bulk of the lysosomal acid hydrolase and peroxidase activity (A); less dense particles, containing all the lysozyme activity, but not resolved from a second population of azurophils B, and particles of low density, biochemically characterized as a plasma membrane fraction (C). Isopyknic equilibration in sucrose disclosed a minor membrane fraction (D) of unidentified origin, whereas mitochondria were best resolved on metrizamide gradients.
mesh_terms: D000818:Animals; D002500:Centrifugation, Isopycnic; D006736:Horses; D009504:Neutrophils / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology