A simple method for the assay of urinary metanephrines using high performance liquid chromatography with fluorescence detection.
A method is described which extends a high performance liquid chromatographic assay for urinary catecholamines to the assay of urinary metanephrines. The amines are separated from urine after acid hydrolysis of conjugates by ion-exchange chromatography, and then further purified by solvent extraction. The final extracts are suitable for direct HPLC assay using the endogenous fluorescence of the amines for detection. Twenty-four hour excretion of the amines in 35 hospital in-patients was found to be (mean and range: 169 micrograms/24 h normetanephrine (39-423), 102 micrograms/24 h metanephrine (19-290) and 138 micrograms/24 h 3-methoxytyramine (32-234)).
D002395:Catecholamines / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004298:Dopamine / Q000031:analogs & derivatives* / Q000652:urine; D004837:Epinephrine / Q000031:analogs & derivatives*; D006801:Humans; D006868:Hydrolysis; D008676:Metanephrine / Q000652:urine*; D009647:Normetanephrine / Q000652:urine*; D013050:Spectrometry, Fluorescence
D002395:Catecholamines; D009647:Normetanephrine; D008676:Metanephrine; C001746:3-methoxytyramine; D004298:Dopamine; D004837:Epinephrine