title: Improved liquid-chromatography of aspirin, salicylate, and salicyluric acid in plasma, with a modification for determining aspirin metabolites in urine.
abstract: Detailed performance specifications are given for a specific and sensitive liquid-chromatographic assay for aspirin, salicylic acid, and salicyluric acid in plasma. The sensitivity of this method for aspirin (50 micrograms/L) is 10-fold that of previous methods, so that concentrations of aspirin in plasma can now be followed for about four or five half-lives after the peak plasma concentration arising from a single 650-mg dose. In addition, this assay is as sensitive for salicylic and salicyluric acid in plasma as any hitherto. A modification permits measurement of gentisic acid, salicylic acid, and salicyluric acid in urine; further modifications allow indirect measurement of conjugated gentisate and salicylate in urine.
mesh_terms: D001241:Aspirin / Q000097:blood* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D005998:Glycine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D006626:Hippurates / Q000097:blood* / Q000652:urine; D006801:Humans; D012016:Reference Values; D012459:Salicylates / Q000097:blood* / Q000652:urine
chemical_list: D006626:Hippurates; D012459:Salicylates; C000693:salicylurate; D001241:Aspirin; D005998:Glycine