Tumor promoter-stimulated synthesis of Mason-:Pfizer monkey virus.
The tumor promoter 12-0-tetradecanoyl-phorbol-14-acetate (TPA) increases by severalfold the synthesis of Mason-Pfizer monkey virus (MPMV), a type D retrovirus, when the virus is growing in human embryo kidney (HEK) cells. The effect is transient and paralleled by a striking morphological alteration of the cells. The optimal TPA concentration for stimulation is 5 ng. ml-1. Contrary to infected HEK cells, TPA induces at similar concentrations neither stimulation of MPMV synthesis nor altered morphology in persistently MPMV-infected cells of the continuous human tumor cell line A 204.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D006801:Humans; D008253:Macaca mulatta / Q000382:microbiology; D010704:Phorbols / Q000494:pharmacology*; D012190:Retroviridae / Q000187:drug effects*; D012208:Rhabdomyosarcoma / Q000382:microbiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014775:Virus Activation / Q000187:drug effects*; D014776:Virus Cultivation; D014777:Virus Diseases / Q000382:microbiology
D010704:Phorbols; D013755:Tetradecanoylphorbol Acetate