A quantitative cytotoxicity assay using whole blood: application to H-2 tissue typing.
A fluorochromatic cytotoxicity assay featuring direct use of whole blood and quantitative evaluation is described. Its application to tissue typing for H-2 antigens together with data indicating reproducibility specificity of the assay are presented. Procedures for visual and automated quantification are included.
D000818:Animals; D001773:Blood Cells; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D005434:Flow Cytometry; D006183:H-2 Antigens; D006650:Histocompatibility Testing; D007106:Immune Sera / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains
D006183:H-2 Antigens; D007106:Immune Sera