title: Opsonic requirements for phagocytosis of Borrelia hermsii by human polymorphonuclear leukocytes.
abstract: Opsonic requirements for phagocytosis by human polymorphonuclear leukocytes (PMNLs) of a laboratory strain of Borrelia hermsii were examined. Intracellular localization of spirochetes was confirmed by electron microscopy and cinephotomicroscopy. Phagocytosis of 3H-labeled spirochetes was increased with higher concentrations of pooled human serum or greater ratios of spirochetes to PMNLs and was unaffected by diminished classical complement pathway activity. Immune rabbit serum markedly increased phagocytosis of spirochetes beyond levels observed with preimmune rabbit serum. Immune rabbit serum also demonstrated direct activity against B. hermsii, which decreased the motility and viability and increased agglutination of the spirochetes independently of complement. Both the direct activity against B. hermsii and the opsonic activity of immune rabbit serum were eliminated by immunoabsorption of immunoglobulins. These observations suggest a role for the alternative complement pathway and for immunoglobulins in the opsonization of spirochetes for phagocytosis by PMNLs. Immunoglobulins may also play a role in the direct clearance of spirochetes.
mesh_terms: D000818:Animals; D001898:Borrelia / Q000276:immunology* / Q000502:physiology; D006358:Hot Temperature; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007136:Immunoglobulins / Q000502:physiology; D066298:In Vitro Techniques; D007700:Kinetics; D008854:Microscopy, Electron; D009504:Neutrophils / Q000276:immunology*; D009895:Opsonin Proteins / Q000502:physiology*; D010587:Phagocytosis; D011817:Rabbits
chemical_list: D007106:Immune Sera; D007136:Immunoglobulins; D009895:Opsonin Proteins