Age-related changes in rat glomerular basement membrane.
1. The yield of GBM from rat kidney increased with rat age and the membrane became more collagen-like, as judged by the glycine, hydroxyproline, hydroxylysine content and the degree of glycosylation. 2. GBM became progressively less soluble in sodium dodecyl sulphate (SDS) and 2-mercaptoethanol with age. An increase in the lower molecular weight subunits of membranes prepared from older rats was demonstrated by polyacrylamide gel electrophoresis.
D000375:Aging; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001485:Basement Membrane / Q000378:metabolism / Q000648:ultrastructure; D004187:Disaccharides / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007678:Kidney Glomerulus / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008623:Mercaptoethanol; D051381:Rats; D011919:Rats, Inbred Strains; D012967:Sodium Dodecyl Sulfate; D012995:Solubility
D000596:Amino Acids; D004187:Disaccharides; D012967:Sodium Dodecyl Sulfate; D008623:Mercaptoethanol