title: Muscarinic receptor binding in the mouse heart: the selective modulatory effect of fluoride ion on agonist binding.
abstract: The effect of fluoride ion on the binding of the specific muscarinic agonist ligand [3H]cis methyldioxolane ([3H]CD) to the mouse cardiac muscarinic receptor was investigated. Utilizing equilibrium ligand binding experiments, sodium fluoride (10mM) was shown to decrease [3H]CD binding, measured at a concentration of 2 nM, by 52%. Studies with several different ions demonstrated that the reduction in [3H]CD binding was a specific effect of fluoride. This fluoride modulation was selective for agonist binding, as no effect of fluoride on the binding of the muscarinic antagonist [3H](-) quinuclidinyl benzilate (QNB) was observed.
mesh_terms: D000818:Animals; D004148:Dioxolanes / Q000378:metabolism; D005459:Fluorides / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects; D007700:Kinetics; D008297:Male; D051379:Mice; D009206:Myocardium / Q000378:metabolism*; D010277:Parasympathomimetics / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism*
chemical_list: D004148:Dioxolanes; D010277:Parasympathomimetics; D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; C024310:2-methyldioxolane; D005459:Fluorides