title: Purification and properties of glucosaminephosphate isomerase of Proteus mirabilis.
abstract: A glucosamine-P isomerase has been identified in Proteus mirabilis. The 113-fold purified enzyme exhibits a pH optimum of 7.5 with a secondary maximum at 8.5 and a temperature optimum at 37 degrees C. The apparent Km was 13.3 mM for fructose-6-P and 18.8 mM for L-glutamine. Molecular weight of the enzyme has been estimated as 120 000 and the protein can be dissociated in four subunits by SDS-polyacrylamide electrophoresis.
mesh_terms: D002238:Carbohydrate Epimerases / Q000302:isolation & purification*; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing) / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D011513:Proteus mirabilis / Q000201:enzymology*
chemical_list: D046911:Macromolecular Substances; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing); D002238:Carbohydrate Epimerases