Evaluation of the Lumac kit for the detection of bacteriuria by bioluminescence.
Four hundred and twenty-two urine samples were screened for significant bacteriuria using bioluminescence and microscopy of uncentrifuged urine. A smaller number of false-negatives were seen with bioluminescence (10%) than with microscopy (40%) while both techniques gave a similar number of false-positives (18%). The kit required a large amount of manual preparation, largely pipetting. With this and the short shelf-life of the reconstituted reagents, it is not suitable for small numbers of urines. At 45p per urine, the cost of bioluminescence is too high.
D000255:Adenosine Triphosphate / Q000032:analysis; D001419:Bacteria / Q000032:analysis; D001437:Bacteriuria / Q000175:diagnosis*; D005069:Evaluation Studies as Topic; D006801:Humans; D008163:Luminescent Measurements; D008853:Microscopy; D011933:Reagent Kits, Diagnostic; D011934:Reagent Strips; D014556:Urine / Q000382:microbiology
D011933:Reagent Kits, Diagnostic; D011934:Reagent Strips; D000255:Adenosine Triphosphate