Phagocytosis of Herpetomonas samuelpessoai by mouse peritoneal macrophages: effect of lidocaine, concanavalin A and carbohydrates.
Normal mouse peritoneal macrophages, as well as macrophages treated with Concanavalin A (Con A) and lidocaine, were incubated in the presence of normal, Con A-treated or glutaraldehyde-fixed Herpetomonas samuelpessoai. The percentage of infected macrophages, the mean number of protozoa per macrophage and the phagocytic index was determined after 1 and 2 h of protozoa-macrophage interaction. The uptake of H. samuelpessoai by the macrophages was increased when either the protozoa or the macrophages or both were treated with Con A. The effect of Con A was dependent on its concentration and was inhibited by alpha-methyl-D-mannoside. The uptake of H. samuelpessoai by macrophages was significantly inhibited by xylose, mannose and a polysaccharide isolated from the portozoan. Lidocaine-treated macrophages incorporated less protozoa than normal macrophages. Both Con A and lidocaine induced the formation of a large number of cytoplasmic vacuoles in the macrophages. Glutaraldehyde-fixed protozoa were more easily incorporated by the macrophages than living cell. However, their uptake was not stimulated by treatment of the macrophages with Con A.
D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology*; D056890:Eukaryota; D008012:Lidocaine / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D010587:Phagocytosis; D013329:Structure-Activity Relationship; D014617:Vacuoles / Q000648:ultrastructure
D002241:Carbohydrates; D003208:Concanavalin A; D008012:Lidocaine