Recognition of base pairs by polar peptides in double stranded DNA.
The resonance Raman spectrum of native DNA has been obtained using excitation at 257 nm. In a first part, the spectral lines are assigned to the different nucleotide bases which provide the resonance effect. In a second part, the interactions of DNA with basic peptides (Arginine Methylester, Lysine Methylester, Arginyl-Arginine) are investigated using excitation at 300 nm and 257 nm, which give complementary information about the DNA. Both Arginine Methylester and Arginyl-arginine recognize the A-T base pairs, the first one in the large groove, the second one in the narrow groove of DNA. The DNA-Lysine Methylester interaction is very likely not specific but can take place in the large groove of DNA.
D000818:Animals; D001482:Base Composition; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA; D004151:Dipeptides; D010455:Peptides; D011485:Protein Binding; D013059:Spectrum Analysis, Raman; D013329:Structure-Activity Relationship; D013950:Thymus Gland
D004151:Dipeptides; D010455:Peptides; D004247:DNA