[Effect of transformed steroid compounds on mRNA synthesis and glucocorticoid-receptor interaction in thymocytes of adrenalectomized rats].
Experiments on thymocyte culture of adrenalectomized rats were made to study the effect of 50 transformed steroid compounds containing delta 4-3-keto or delta 5-3-hydroxygroup in ring A, oxygen-containing substituents in ring D and superstructed 20-keto-side chain on glucocorticoid and antiglucocorticoid effects and steroid-receptor interaction. Ten compounds given in a concentration of 10(6) M competed with 3H-triamcinolon for binding thymocyte cytosol on glucocorticoid receptors. Steroid compounds containing the delta 4-3-ketogroup inhibit more actively the incorporation of 3H-uridine into RNA in a concentration of 10(6) M, as compared with compounds containing the delta 5-3-hydroxygroup. Antiglucocorticoid effect on thymocyte function is also produced by 16,17 alpha-isopropylidenedioxy-pregn-5-en-20-on-3 beta-ol; 16,17 alpha-isopropylidenedioxy-pregna-5,21 (21a)-dien-20-on-3 beta-ol at a concentration of 10(6) M.
D000311:Adrenal Glands / Q000502:physiology*; D000315:Adrenalectomy; D000818:Animals; D003600:Cytosol / Q000378:metabolism; D006914:Hydroxysteroids / Q000494:pharmacology; D066298:In Vitro Techniques; D007664:Ketosteroids / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D013256:Steroids / Q000494:pharmacology*; D013950:Thymus Gland / Q000166:cytology* / Q000378:metabolism; D014222:Triamcinolone Acetonide / Q000378:metabolism
D006914:Hydroxysteroids; D007664:Ketosteroids; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D013256:Steroids; D014222:Triamcinolone Acetonide
Vliianie transformirovannykh steroidnykh soedineniĭ na sintez mRNK i gliukokortik gliukokortikoidretseptornoe vzaimodeĭstvie v timotsitakh adrenaléktomirovannykh krys.