Monoclonal immunoglobulin of CLL lymphocytes detected by radioimmunofixation with 125I-protein A of Triton X-100 solubilized lymphocyte material.
A sensitive radioimmunofixation method (detection limit 1 mg/1 for monoclonal IgM) utilizing 125I Protein A and immunofixation for demonstration of monoclonal immunoglobulin in Triton X-100 extracts of isolated chronic lymphocytic leukemia (CLL) lymphocytes is presented. Thirty-two untreated patients with clinically typical CLL were studied and the results compared to those obtained by immunofluorescence. Monoclonal IgM was detected with similar frequency by the two methods (69% and 75%), but IgD was detected less frequently by radioimmunofixation (25% and 40%). When the results obtained with the two methods were combined, all of the cases were immunoglobulin positive. Two complete IgM type M-components were demonstrated in two cases, an excess of seemingly free heavy chains was found in four cases, and an excess of seemingly free light chains in six cases.
D000911:Antibodies, Monoclonal / Q000032:analysis*; D001345:Autoradiography; D001798:Blood Proteins / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis; D007136:Immunoglobulins; D007158:Immunologic Techniques; D007457:Iodine Radioisotopes; D007945:Leukemia, Lymphoid / Q000032:analysis*; D008214:Lymphocytes / Q000032:analysis*; D011092:Polyethylene Glycols / Q000494:pharmacology; D013205:Staphylococcal Protein A / Q000378:metabolism
D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D007075:Immunoglobulin M; D007136:Immunoglobulins; D007457:Iodine Radioisotopes; C009823:M-proteins (Myeloma); D013205:Staphylococcal Protein A; D011092:Polyethylene Glycols