Molecular ferrokinetics in the rabbit.
Using urea-polyacrylamide gel electrophoresis it has been possible to distinguish the molecular forms of transferrin in rabbit serum. When 59Fe-labelled diferric transferrin is injected into normal, anaemic or hypertransfused, polycythaemic rabbits, iron is removed from diferric transferrin in essentially pairwise fashion. Exchange of iron between transferrin and tissues was also studied using predominantly monoferric transferrin labelled with 59Fe or 125I, and with 125I-labelled apotransferrin. The return of iron from tissue stores to circulating transferrin occurs one atom at a time to either site of the protein and, possibly, in pairwise fashion as well. The rate of clearance of iron from diferric transferrin differs from that of monoferric transferrins, and the rates at which iron is returned to empty sites of transferrin also differ, so that serum iron is not a kinetically homogeneous pool in the rabbit.
D000740:Anemia / Q000097:blood; D000818:Animals; D001059:Apoproteins / Q000097:blood; D005290:Ferric Compounds / Q000097:blood; D007457:Iodine Radioisotopes; D007501:Iron / Q000097:blood*; D007504:Iron Radioisotopes; D007700:Kinetics; D008297:Male; D011086:Polycythemia / Q000097:blood; D011817:Rabbits; D014168:Transferrin / Q000378:metabolism*
D001059:Apoproteins; D005290:Ferric Compounds; D007457:Iodine Radioisotopes; D007504:Iron Radioisotopes; D014168:Transferrin; C028284:apotransferrin; D007501:Iron