title: Isolation and partial characterisation of three major allergens in an extract from cow hair and dander.
abstract: Three 'major allergens' of an extract from cow hair and dander were isolated by means of anion-exchange chromatography and hydrophobic chromatography. Two of the isolated 'major allergens' were immunochemically pure and the third contained less than 1% impurities. The molecular weights were 2.0, 2.2 and 2.4 x 10(4) daltons, and pI was about 4.0. The amino acid content revealed no specific traits. The protein part of the major allergens constituted about 60% (w/w). The allergenic activity of the isolated allergens was verified in RAST-inhibition experiments and in prick tests.
mesh_terms: D000485:Allergens / Q000302:isolation & purification*; D000596:Amino Acids; D000818:Animals; D001249:Asthma / Q000175:diagnosis / Q000276:immunology; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002852:Chromatography, Ion Exchange; D004220:Disulfides / Q000494:pharmacology; D005260:Female; D006197:Hair / Q000276:immunology*; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D011817:Rabbits; D011852:Radioallergosorbent Test; D011952:Receptors, Concanavalin A; D012882:Skin Tests
chemical_list: D000485:Allergens; D000596:Amino Acids; D004220:Disulfides; D011952:Receptors, Concanavalin A; D011092:Polyethylene Glycols; D017830:Octoxynol