Isolation and characterization of the C3b-binding entity of C3b-receptor from human erythrocytes.
Applying 2 M KBr, membranes of Ehu were solubilized. By C3-affinity chromatography an activity could be isolated that inhibited the immune adherence reaction and C3b-dependent rosette formation. Since this material did not agglutinate EAC14oxy23b we termed it monovalent C3b receptor (mC3bR). PAGE and SDS-PAGE and staining with Coomassie brilliant blue and PAS reagent revealed a single glycoprotein band with a mol. wt. of 55,000-60,000 daltons and an electrophoretic mobility comparable to ovalbumin. This mC3bR proved to be antigenetically related to gp 205 [17]. The potential of mC3bR to react with C3b-carrying particles was not destroyed by heat and trypsin treatment but by neuraminidase or periodic acid treatment suggesting that mC3bR reacted by its carbohydrate moiety with C3b. As by mC3bR, immune adherence could be inhibited by D-glucose and D-galactose but not by their optical antipodes, L-glucose and L-galactose.
D000818:Animals; D000941:Antigens / Q000276:immunology; D055598:Chemical Phenomena; D002621:Chemistry; D003179:Complement C3b / Q000276:immunology / Q000302:isolation & purification*; D003180:Complement C3b Inactivator Proteins / Q000494:pharmacology; D004912:Erythrocytes / Q000032:analysis* / Q000276:immunology; D006168:Guinea Pigs; D006801:Humans; D007104:Immune Adherence Reaction; D009005:Monosaccharides / Q000494:pharmacology; D011817:Rabbits; D011951:Receptors, Complement / Q000302:isolation & purification*; D012397:Rosette Formation
D000941:Antigens; D003180:Complement C3b Inactivator Proteins; D009005:Monosaccharides; D011951:Receptors, Complement; D003179:Complement C3b