Preparation of hepatitis B polypeptide micelles from human carrier plasma.
Water-soluble protein micelles consisting of the 28,000 (gp28) and 23,00 (p23) molecular weight polypeptide complex of hepatitis B surface antigen can be readily prepared from human plasma containing the surface antigen and other markers of infection with hepatitis B virus. The antigenic activity of the polypeptides was preserved throughout the process of solubilisation and reassociation into micelles. Such preparations are therefore eminently suitable as "second generation" hepatitis B vaccines.
D002353:Carrier State / Q000276:immunology; D002499:Centrifugation, Density Gradient; D003102:Colloids; D006023:Glycoproteins / Q000276:immunology*; D006509:Hepatitis B / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D008823:Micelles; D014764:Viral Proteins / Q000276:immunology*
D003102:Colloids; D006023:Glycoproteins; D006514:Hepatitis B Surface Antigens; D008823:Micelles; D014764:Viral Proteins