title: Drawbacks in the use of 23-nor-deoxycholic acid standards.
abstract: 23-Nor-deoxycholic acid is widely used as internal standard in gas-liquid chromatographic studies of bile acids. Two batches of this compound, submitted to conventional alkaline hydrolysis of bile acid conjugates, were found to be transformed into a product with chromatographic properties different from those of "authentic" 23-nor-deoxycholic acid. To identify this "new" product a comparison was made between chromatographic properties, mass spectra, and NMR spectra of 23-nor-deoxycholic acid before and after alkaline hydrolysis. The results indicate that the "new" compound is the true 23-nor-deoxycholic acid while the product present in the two batches examined is a monoacetate derivative.
mesh_terms: D003840:Deoxycholic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D012015:Reference Standards
chemical_list: D003840:Deoxycholic Acid; C033582:23-nordeoxycholic acid