Effect of estradiol and degree of cell differentiation on human melanoma susceptibility to killing by monoclonal antibodies.
An estradiol non-responsive melanotic (E-M+ShA) was derived from an estradiol-responsive melanotic (E+M+ShA), and an estradiol-responsive amelanotic (E+M-SR) was derived from estradiol non-responsive amelanotic (E-M-SR) cell lines by deprivation and supplementation of the culture medium with estradiol, respectively. E+M+ShA cells became E-M+ShA after 5 weeks, i.e. 2 subcultures, of culture in the absence of estrogen, and E-M-SR cells became E+M-SR after 7 weeks, i.e. 2 subcultures, of culture in the presence of 10(-7) M 17 beta-estradiol. Spleen lymphocytes from mice pre-immunized with E+M+ShA, E-M+ShA, E-M-SR and E+M-SR have been fused with murine non-producer myeloma cells to obtain mouse-mouse hybridoma cultures that synthesize monoclonal antibodies against human malignant melanoma. The complement-dependent cytotoxin activities of these antibodies linked responsiveness to estradiol with melanotropin biosynthesis.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D003165:Complement System Proteins / Q000378:metabolism; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004958:Estradiol / Q000494:pharmacology*; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology; D009074:Melanocyte-Stimulating Hormones / Q000494:pharmacology; D008545:Melanoma / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013936:Thymidine / Q000378:metabolism
D000911:Antibodies, Monoclonal; D004958:Estradiol; D009074:Melanocyte-Stimulating Hormones; D003165:Complement System Proteins; D013936:Thymidine