title: [Bovine liver glutamate dehydrogenase modified by 2,2,6,6-tetramethyl-4-oxopiperidine-1-oxyl: enzymatic activity and catalytic properties].
abstract: When modified by 2,2,6,6-tetramethyl-4-oxopiperidine-1-oxyl (TMPO) bovine liver glutamate dehydrogenase (L-glutamate NAD(P) oxidoreductase, E. C. 1.4.1.3) looses its catalytical activity and sensitivity to allosteric inhibitor GTP. The stoicheiometry of the binding of TMPO to glutamate dehydrogenase has been studied--each protomer bound one molecule of TMPO. It is supposed that TMPO reacts with lysine residue located in the enzyme's active center.
mesh_terms: D000494:Allosteric Regulation; D000818:Animals; D002417:Cattle; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D013113:Spin Labels / Q000494:pharmacology*
chemical_list: D003497:Cyclic N-Oxides; D013113:Spin Labels; D006160:Guanosine Triphosphate; D005969:Glutamate Dehydrogenase; C003959:TEMPO
vernacular_title: Modifikatsiia glutamatdegidrogenary pecheni byka 2,2,6-6-tetrametil-4-okso-piperidin-1-oksilom. Vliianie na aktivnost' i kataliticheskie svoĭstva.