title: Pig heart [35S]thiophosphoryl pyruvate dehydrogenase complexes.
abstract: Thiophosphorylation of the pig heart pyruvate dehydrogenase complex with ATP [35S] (adenosine 5'[gamma-thio]-triphosphate) was analogous to phosphorylation with [gamma-32P]ATP except that thiophosphorylation of sites 2 and 3 was more rapid. With pyruvate dehydrogenase phosphatase the rate of dethiophosphorylation was 0.5% of that of dephosphorylation. Thiophosphorylation of sites 2 and 3 in complex phosphorylated in site 1 reduced the rate of dephosphorylation of site 1.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D009206:Myocardium / Q000201:enzymology*; D010766:Phosphorylation; D011768:Pyruvate Dehydrogenase Complex / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D013455:Sulfur / Q000378:metabolism; D013552:Swine
chemical_list: D011768:Pyruvate Dehydrogenase Complex; D013455:Sulfur; D000255:Adenosine Triphosphate