Effects of lipid peroxidation on prostaglandin synthesis in rabbit kidney medulla slices.
Ascorbic acid and Fe2+ had a more powerful stimulatory effect on the lipid peroxidation of rabbit kidney medullar slices in 0.15 M KCl/0.02 M Tris-HCl buffer than in Krebs-Henseleit buffer. The lipid peroxidation induced by ascorbic acid and Fe2+ enhanced the release of unsaturated fatty acids from tissue lipids, but inhibited medullary generation of prostaglandin E. These results suggest that the lipid peroxidation induced by ascorbic acid and Fe2+ may inhibit the formation of prostaglandin E and that this inhibitory effect may be mediated by lipid peroxides via the inhibition of prostaglandin synthetase.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D007501:Iron / Q000494:pharmacology; D007679:Kidney Medulla / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D011453:Prostaglandins / Q000096:biosynthesis*; D011817:Rabbits
D008054:Lipid Peroxides; D011453:Prostaglandins; D007501:Iron; D001205:Ascorbic Acid