Surface components of ejaculated chimpanzee sperm.
Surface components of ejaculated chimpanzee sperm were radiolabeled by enzymatic iodination with lactoperoxidase and Na125I. The sperm were lysed and the labeled components analyzed on SDS-7.5% polyacrylamide gels. Electrophoresis of solubilized radioactivity resolved four components with approximate molecular weights of 72,000, 49,000, 25,000 and 17,000 daltons. To identify components, which might be adsorbed to the chimpanzee sperm surface from seminal secretions, seminal plasma was labeled. Electrophoresis of labeled seminal plasma resolved three components with approximate molecular weights of 63,000, 26,000, and 17,000 daltons, two of which comigrated with a labeled sperm surface component.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004542:Ejaculation; D004591:Electrophoresis, Polyacrylamide Gel; D007784:Lactoperoxidase / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D017830:Octoxynol; D002679:Pan troglodytes; D011092:Polyethylene Glycols; D012661:Semen / Q000032:analysis; D012974:Sodium Iodide; D013094:Spermatozoa / Q000032:analysis*; D013499:Surface Properties
D011092:Polyethylene Glycols; D017830:Octoxynol; D007784:Lactoperoxidase; D012974:Sodium Iodide