Enrichment for auxotrophic mutants in Saccharomyces cerevisiae using the cell wall inhibitor, echinocandin B.
Echinocandin B has been shown to inhibit fungal cell wall synthesis. This report describes the use of echinocandin B to enrich for nutritional auxotrophs in a mutagenized strain of Saccharomyces cerevisiae. Up to 20-fold enrichment levels for total auxotrophs were achieved after a single round of treatment with echinocandin; this level of enrichment is the highest of all procedures for which a specific mutant strain is not required.
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D002473:Cell Wall / Q000187:drug effects*; D054714:Echinocandins; D005656:Fungal Proteins; D009154:Mutation; D010455:Peptides / Q000494:pharmacology; D010456:Peptides, Cyclic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012641:Selection, Genetic
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D054714:Echinocandins; D005656:Fungal Proteins; D010455:Peptides; D010456:Peptides, Cyclic; C007572:echinocandin B