title: Synthesis and degradation of phenylalanine ammonia-lyase of Rhodosporidium toruloides.
abstract: The regulation of the enzyme phenylalanine ammonia-lyase (PAL), which is of potential use in oral treatment of phenylketonuria, was investigated. Antiserum against PAL was prepared and was shown to be monospecific for the enzyme by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The native enzyme and two inactive mutant forms of the enzyme were purified to homogeneity by immunoaffinity chromatography, using anti-PAL immunoglobulin G-Sepharose 4B. Both mutant enzymes contained intact prosthetic groups. The formation of PAL catalytic activity after phenylalanine was added to yeast cultures was paralleled by the appearance of enzyme antigen. During induction, uptake of [3H]leucine into the enzyme was higher than uptake into total protein. Our results are consistent with de novo synthesis of an enzyme induced by phenylalanine, rather than activation of a proenzyme. The half-lives of PAL and total protein were similar in both exponential and stationary phase cultures. No metabolite tested affected the rate of enzyme degradation. Glucose repressed enzyme synthesis, whereas ammonia reduced phenylalanine uptake and pool size and so may repress enzyme synthesis through inducer exclusion. The synthesis of enzyme antigen by a mutant unable to metabolize phenylalanine indicated that this amino acid is the physiological inducer of the enzyme.
mesh_terms: D000641:Ammonia / Q000494:pharmacology; D000642:Ammonia-Lyases / Q000378:metabolism*; D001487:Basidiomycota / Q000201:enzymology*; D004790:Enzyme Induction; D005947:Glucose / Q000494:pharmacology; D006207:Half-Life; D007700:Kinetics; D009154:Mutation; D010649:Phenylalanine / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D010649:Phenylalanine; D000641:Ammonia; D000642:Ammonia-Lyases; D010650:Phenylalanine Ammonia-Lyase; D005947:Glucose