[The importance of the position of the adenosine linkage in NAD+ for its coenzyme activity via lactate dehydrogenase (author's transl)].
We report on the chemical preparation of the NAD+ analog P1, P2-(5'-beta-nicotinamideribofuranosyl-3"-adenosyl)-diphosphate (N3"AD+) by applying the 1,1'-carbonyldiimidazole activation method. The importance of how adenosine is linked to the nicotinamide mononucleotide phosphoriboride (NMNPR+) moiety is discussed with respect to the coenzyme activity in the lactate dehydrogenase catalyzed reaction.
D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D009243:NAD / Q000031:analogs & derivatives* / Q000494:pharmacology; D013329:Structure-Activity Relationship
D009243:NAD; D007770:L-Lactate Dehydrogenase
Die Bedeutung der Verknüpfungsposition des Adenosins im NAD+ für die Coenzymaktivität gegenüber Lactatdehydrogenase.