[Chromatographic and spectral characteristics of liposoluble fluorescent compounds accumulated during damaging and aging of plants].
The chromatographic properties (e. g. thin layer mobility, separation by molecular adsorption and gel chromatography) and the fluorescence parameters of liposoluble fluorescent compounds (LFC) accumulated in the tissues during aging of detached leaves, in autumn leaves of deciduous plants and in the leaves treated with diquat, 2,4-dichloro- and 2,4,5-trichlorophenoxyacetic acids were studied. The peculiarities of detection and analysis of LFC in plant tissues are discussed. Fractionation of individual components of LFC was carried out and their properties were compared. The properties of LFC were compared to those of lipofuscine-like animal pigments and fluorescent products formed by NADPH-dependent lipid peroxidation system in rat liver microsomes stimulated by reduced pyridine nucleotides in pea leaf microsomes and by lipid photoperoxidation in isolated chloroplasts. The data obtained suggest that accumulation of "heavy" LFC fractions can reflect the enhancement of the peroxidative degradation processes involving unsaturated lipids in damaged and aging plant cells.
D000818:Animals; D008054:Lipid Peroxides / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010860:Pigments, Biological / Q000302:isolation & purification* / Q000378:metabolism; D063245:Plant Development; D010944:Plants / Q000378:metabolism; D051381:Rats; D012995:Solubility; D013050:Spectrometry, Fluorescence
D008054:Lipid Peroxides; D010860:Pigments, Biological; D009249:NADP
Khromatograficheskie i spektral'nye kharakteristiki liporastvorimykh fluorestsiruiushchikh soedineniĭ, nakaplivaiushchikhsia pri povrezhdenii i starenii tkaneĭ rastenii.