Histone proteins in HeLa S3 cells are synthesized in a cell cycle stage specific manner.
The synthesis of histone proteins in G1 and S phase HeLa S3 cells was examined by two-dimensional electrophoretic fractionation of nuclear and total cellular proteins. Newly synthesized histones were detected only in S phase cells. Histone messenger RNA sequences, as detected by hybridization with cloned human histone genes, were present in the cytoplasm of S phase but not G1 cells.
D002453:Cell Cycle; D002467:Cell Nucleus / Q000378:metabolism; D003561:Cytarabine / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D006367:HeLa Cells / Q000378:metabolism*; D006657:Histones / Q000096:biosynthesis*; D006801:Humans; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
D006657:Histones; D003561:Cytarabine