Analysis of stage-specific protein synthesis during spermatogenesis of the mouse by two-dimensional gel electrophoresis.
Cells were labelled by intratesticular injection of [35S]methionine. After 14-16 h the relative rates of incorporation of label in spermatocytes, early spermatids and late spermatids were 10:2:1 respectively. Approximately 15% of the soluble (100 000 g supernatant) and 20% of the particulate proteins solubilized by NP-40 (from the 100 000 g pellet) that were detectable on two-dimensional gels showed stage-specific synthesis. A large number of proteins were detectable only in post-meiotic cells and may be valuable for studying the control of gene expression in haploid cells.
D000818:Animals; D001345:Autoradiography; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008715:Methionine / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D014176:Protein Biosynthesis; D013087:Spermatids / Q000378:metabolism*; D013090:Spermatocytes / Q000378:metabolism*; D013091:Spermatogenesis; D013094:Spermatozoa / Q000378:metabolism*
D008715:Methionine