Evidence that stimulatory factor(s) of RNA polymerase II participates in accurate transcription in a HeLa cell lysate.
Antibody raised against S-II, a stimulatory factor of RNA polymerase II from Ehrlich ascites tumor cells, inhibited accurate transcription from adenovirus 2 major late promoter in a HeLa cell lysate. Manganese prevented accurate transcription, but it was essential for expression of the stimulatory activity of S-II. The results indicate that protein(s) cross-reacting immunologically with S-II in a HeLa cell lysate is essential for accurate transcription of truncated DNA, but the stimulatory activity of this factor(s) is not necessary for accurate transcription of truncated DNA.
D000818:Animals; D000936:Antigen-Antibody Complex; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D002467:Cell Nucleus / Q000201:enzymology; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007106:Immune Sera; D008345:Manganese / Q000494:pharmacology; D051379:Mice; D012319:RNA Polymerase II / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D008345:Manganese; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases