Separation of human skin cells by velocity sedimentation into functionally distinct fractions.
The epidermis consists of a heterogeneous population of cells including Langerhans cells, Merkel cells, melanocytes, and keratinocytes in various stages of differentiation. The current study was undertaken to determine if skin cell suspensions can be separated into morphologically and/or functionally distinct fractions. Skin cells were suspended by trypsinization and separated into multiple fractions by velocity sedimentation. Certain fractions reproducibly stimulated proliferation of allogeneic lymphocytes in the skin cell lymphocyte reaction, whereas other fractions, containing larger cells, supported growth of keratinocyte colonies in cell cultures. These results indicate that stimulation in the skin cell lymphocyte reaction and growth of keratinocyte colonies are mediated by distinct cells, separable by velocity sedimentation.
D002455:Cell Division; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D006801:Humans; D008213:Lymphocyte Activation; D012867:Skin / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure