Isolation and characterization of goat submaxillary-mucin.
Mucin from the submaxillary glands of goats was isolated by precipitation from an aqueous extract with a cationic detergent, followed by dissolution in CaCl2 solution, and fractionation on a DEAE-cellulose column, Five homogeneous, mucin fractions were isolated by NaCl-gradient elution of the column. One of the fractions was obtained in major yield (46%); it was homogeneous by paper electrophoresis, and contained carbohydrates (46.4%) and protein (48.9%). The sugars were fucose, galactose, glucose, mannose, 2-acetamido-2-deoxygalactose, 2-acetamido-2-deoxyglucose, and N-acetylneuraminic acid. The principal amino acids were alanine, glutamic acid, glycine, proline, serine, threonine, and valine. The proportions of these residues were determined.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D006041:Goats; D009077:Mucins / Q000302:isolation & purification*; D012794:Sialic Acids / Q000032:analysis; D013363:Submandibular Gland / Q000032:analysis*
D000596:Amino Acids; D002241:Carbohydrates; D009077:Mucins; D012794:Sialic Acids