title: The effects of inorganic salts on the stability of isocitrate dehydrogenase from beef (Bos taurus) heart and liver.
abstract: 1. Distinct differences are established between the two isozymes of NADP+-dependent isocitrate dehydrogenase, purified from beef (BOS taurus) heart and liver, with respect to their net electrical charges and stabilization properties in the presence of inorganic salts. 2. Inorganic salts also inhibit the isozymes with different apparent Ki values. 3. Both isozymes are found to be heat labile in the absence and cold labile in the presence of salts. 4. The results indicate that different mechanisms of heat stabilization exist due to different conformational states which are less active but more stable.
mesh_terms: D000818:Animals; D002417:Cattle; D004355:Drug Stability; D006358:Hot Temperature; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D009928:Organ Specificity; D012492:Salts / Q000494:pharmacology
chemical_list: D007527:Isoenzymes; D012492:Salts; D007521:Isocitrate Dehydrogenase