Regulation of acetylcholinesterase by nerve growth factor in the pheochromocytoma PC12 cell line.
In the PC12 clonal nerve cell line derived from a rat pheochromocytoma, the activity of acetylcholinesterase (AChE) is regulated by nerve growth factor (NGF). The specific activity of AChE was significantly increased 2 days after exposure of PC12 cells to NGF (8 X 10(-8) M), and reached its maximal increase of 94% after 3 days. Thereafter the specific activity of AChE did not significantly vary during the following 7 days. AChE cytochemistry showed the enzyme activity to be localized predominantly in cisternae of the rough endoplasmic reticulum, with a strongly enhanced activity after NGF treatment.
D000110:Acetylcholinesterase / Q000378:metabolism*; D000310:Adrenal Gland Neoplasms / Q000201:enzymology* / Q000648:ultrastructure; D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D007700:Kinetics; D008854:Microscopy, Electron; D009374:Neoplasms, Experimental / Q000201:enzymology / Q000648:ultrastructure; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010673:Pheochromocytoma / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats
D009414:Nerve Growth Factors; D000110:Acetylcholinesterase