The in vitro cultivation of Taenia pisiformis to sexually mature adults.
Taenia pisiformis was cultivated from a cysticercus to a non-fertile adult in a diphasic medium. This medium was composed of coagulated bovine serum, as a solid phase, and CMRL 1066, modified by the addition of fetal calf serum, yeast extract, glucose and KCl, as a liquid phase. The medium was gassed with 10% O2 and 5% CO2 in N2. Evidence of morphogenesis with proglottid formation occurred after 7-8 days of culture, and genital pores were formed after 22 days. Some worms showed signs of teratological changes with two or three genital pores.
D000818:Animals; D001769:Blood; D003470:Culture Media; D003552:Cysticercus / Q000254:growth & development; D005947:Glucose; D009024:Morphogenesis; D010274:Parasitology / Q000379:methods*; D011189:Potassium Chloride; D013621:Taenia / Q000033:anatomy & histology / Q000254:growth & development*; D015002:Yeast, Dried
D003470:Culture Media; D011189:Potassium Chloride; D005947:Glucose