13C-nuclear magnetic resonance studies of sugar cataractogenesis in the single intact rabbit lens.
In this report we demonstrate that 13C-nuclear magnetic resonance spectroscopy may be used to monitor the sorbitol and glycolytic pathways in a single, intact, crystalline lens of the rabbit, thereby providing a new, noninvasive method for the study of sugar cataractogenesis. This approach is particularly useful for the measurement of intralenticular metabolic fluxes in single lenses under various conditions, at a time resolution previously unavailable in lens biochemistry.
D000818:Animals; D002386:Cataract / Q000209:etiology / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D011817:Rabbits; D013012:Sorbitol / Q000378:metabolism*
D013012:Sorbitol; D005947:Glucose