Block of inward rectification by intracellular H+ in immature oocytes of the starfish Mediaster aequalis.
Intracellular pH was recorded in immature starfish oocytes using pH-sensitive microelectrodes, and inwardly rectifying potassium currents were measured under voltage clamp. When the intracellular pH was lowered using acetate-buffered artificial sea water from the normal value of 7.09 to 5.9, inward rectification was completely blocked. The relationship between inward rectification and internal pH between 7.09 and 5.9 could be fit by a titration curve for the binding of three H ions to a site with a pK of 6.26 to block the channel. The H+ block showed no voltage dependence, and the activation kinetics of the inwardly rectifying currents were not affected by the changes in internal pH.
D000818:Animals; D001693:Biological Transport, Active; D005260:Female; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008564:Membrane Potentials; D009865:Oocytes / Q000378:metabolism* / Q000502:physiology; D010063:Ovum / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D012623:Seawater; D013215:Starfish / Q000378:metabolism* / Q000502:physiology
D011188:Potassium