title: Oligoclonal IgG in the cerebrospinal fluid of guinea pigs with acute experimental allergic encephalomyelitis.
abstract: The cerebrospinal fluid (CSF) of guniea pigs with experimental allergic encephalomyelitis was examined for the presence of oligoclonal IgG using polyacrylamide gel electrophoresis. Oligoclonal IgG (greater than or equal to 2 bands) was seen in the CSF obtained from 3/4 animals with experimental allergic encephalomyelitis induced by myelin basic protein and 2/3 with spinal cord-induced disease. It was not seen in CSF of 3 non-sensitized, 4 adjuvant-sensitized and 7 liver-sensitized guinea pigs. Scanning of stained gels confirmed the oligoclonal pattern. The bands were found in the region of gels which bound [125I]Staphylococcal Protein A. The data demonstrate that a non-infectious inflammatory reaction within the central nervous system can result in an oligoclonal IgG pattern in the CSF.
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D006168:Guinea Pigs; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid*; D008297:Male; D013116:Spinal Cord / Q000276:immunology
chemical_list: D007074:Immunoglobulin G