title: Role of vitamin B12 in methyl transfer for methane biosynthesis by Methanosarcina barkeri.
abstract: When Methanosarcina barkeri is grown on methanol as the sole carbon source, a B12-containing protein is synthesized by this organism. This B12 protein contains bound aquocobalamin, and when this cofactor is reduced and methylated with [14C]methyl iodide, the resultant [14C]methyl B12 protein is extremely active in the biosynthesis of 14C-labeled methane. These findings indicate that a B12-dependent system is operative in the biological formation of methane in addition to other systems that are B12-independent.
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D019605:Euryarchaeota / Q000378:metabolism*; D008697:Methane / Q000096:biosynthesis*; D000432:Methanol / Q000378:metabolism; D014805:Vitamin B 12 / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D008697:Methane; D014805:Vitamin B 12; D000432:Methanol