A purified serum glycopeptide from controls and cystic fibrosis patients. I. Comparison of their mucociliary activity on rabbit tracheal explants.
A glycopeptide with a molecular weight of approximately 5000 was purified from sera of controls and cystic fibrosis patients. Both the cystic fibrosis and control glycoproteins revealed the absence of aromatic amino acids. The effect of the purified glycopeptide on rabbit tracheal explants was examined in a double-blind fashion. The purified fraction from cystic fibrosis patients caused marked ciliary dyskinetic activity and profound mucus secretion. The equivalent fraction from controls, even in a 50-fold higher concentrations, showed no apparent biological activity on rabbit tracheal explants.
D000293:Adolescent; D000328:Adult; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002648:Child; D002675:Child, Preschool; D002923:Cilia / Q000502:physiology*; D003550:Cystic Fibrosis / Q000097:blood*; D005260:Female; D006023:Glycoproteins / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008970:Molecular Weight; D009093:Mucus / Q000502:physiology; D011817:Rabbits; D014132:Trachea / Q000502:physiology*
D006023:Glycoproteins