Peptidoglycan of Rhodopseudomonas viridis: partial lack of N-acetyl substitution of glucosamine.
A lack of at least 70% of N-acetyl substitution of glucosamine in the glycan strands of the peptidoglycan from the gram-negative bacterium Rhodopseudomonas viridis is reported. A disaccharide, very likely GlcN beta(1 leads to 4) Mur, was observed in hydrolysates of the isolated peptidoglycan. The disaccharide was not observed when peptidoglycan was N-acetylated before hydrolysis. The peptidoglycan of R. viridis was resistant to lysozyme but became sensitive after N-acetylation with acetic anhydride. The disaccharide was found with peptidoglycan from all R. viridis strains investigated, as well as with R. sulfoviridis P1 and R. palustris strains, but not with peptidoglycan from R. gelatinosa, Rhodospirillum tenue, and Pseudomonas diminuta NCTC 8545.
D000107:Acetylation; D004187:Disaccharides / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006868:Hydrolysis; D009113:Muramidase / Q000494:pharmacology; D010457:Peptidoglycan / Q000032:analysis* / Q000302:isolation & purification; D012241:Rhodopseudomonas / Q000032:analysis*
D004187:Disaccharides; D010457:Peptidoglycan; D009113:Muramidase; D005944:Glucosamine