Distribution of the core histones H2A.H2B.H3 and H4 during cell replication.
The distribution of newly synthesized core histones H2A, H2B, H3 and H4 relative to the DNA strand synthesized in the same generation has been examined in replicating Chinese Hamster ovary cells. Cells are grown for one generation in [14C]-lysine and thymidine, and then for one generation in [3H]-lysine and 5-bromodeoxyuridine (BrUdRib) and a further generation in unlabeled lysine and thymidine. This protocol produces equal amounts of unifilarly substituted and unsubstituted DNA. Monomer nucleosomes isolated from chromatin containing these two types of DNA can be distinguished by crosslinking with formaldehyde and banding to equilibrium in CsCl density gradients. The results indicate that the core histones are equally distributed between the two types of DNA. These findings are discussed in terms of current models for chromatin replication; they do not support any long term association of newly replicated histones with either the leading or lagging side of the replication fork.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002250:Carbon Radioisotopes; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006657:Histones / Q000235:genetics*; D046911:Macromolecular Substances; D009707:Nucleosomes / Q000032:analysis; D010053:Ovary; D014316:Tritium
D002250:Carbon Radioisotopes; D006657:Histones; D046911:Macromolecular Substances; D009707:Nucleosomes; D014316:Tritium; D001973:Bromodeoxyuridine