Factors affecting in vitro DNA ejection of the Lactobacillus lactis bacteriophage LL-H.
Tris(hydroxymethyl)aminomethane (tris) inactivates Lactobacillus lactis bacteriophages LL-H in vitro. The inactivation is caused by DNA ejection. This effect occurs in tris-HCl buffer only. Both pH and temperature affect the sensitivity of the phage to the tris treatment. The divalent cation Mg2+ prevented the inactivating effected of tris at concentrations about 10(3)-fold lower than monovalent cation K+.
D001435:Bacteriophages / Q000187:drug effects / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus; D008274:Magnesium / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D013696:Temperature; D014325:Tromethamine / Q000494:pharmacology*
D004279:DNA, Viral; D014325:Tromethamine; D008274:Magnesium; D011188:Potassium