Incidence of nickel-induced sister-chromatid exchange.
Nickel is a recognized carcinogen to which the general population is being increasingly exposed. A nickel-induced alteration in the incidence of sister-chromatid exchanges in cultured lymphocytes has been investigated. Human lymphocytes from a single donor were cultured in media containing various concentrations of nickelous chloride from 10(-6) to 10(-3) M and a control culture containing no added nickel. A significantly increased incidence of exchanges over background was induced at 1.19 x 10(-4) M as indicated by a Student's t-test. A concentration/response curve was generated up to 5 x 10(-4) M where the nickel concentration was lethal to the cells. The nickel response was analyzed by a chi-square test for any variation in location of activity across the karyotype and no differences were detected. The background incidence did vary from a uniform distribution across the karyotype with increasing incidences in chromosomes 1 and 2 and those of the B group, and decreased incidences in the E, F, and G groups.
D002478:Cells, Cultured; D002877:Chromosomes, Human / Q000187:drug effects*; D003434:Crossing Over, Genetic; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D009153:Mutagens; D009532:Nickel / Q000494:pharmacology*; D012854:Sister Chromatid Exchange
D009153:Mutagens; D009532:Nickel