Salt extractability of nuclear receptor-estrogen complex from the rat uterus.
Estrogen receptors in the nuclei of rat uterus were determined 1 or 6 h after the injection of [3H]estradiol. Ratio of 0.4 M KCl-extractable to -resistant receptor-estradiol complex was constant during this time interval. Three consecutive extractions took out about 95% of receptor-estradiol complex either 1 or 6 h after injection. Extraction with KCl before the exchange assay reduced the amount of salt-resistant nuclear receptor-estradiol complex. However, when exchange incubation with [3H] estradiol was done before KCl extraction, salt-resistant receptor-estradiol complex was significantly increased, even after three consecutive extractions.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D004958:Estradiol / Q000378:metabolism; D005260:Female; D011189:Potassium Chloride; D051381:Rats; D011919:Rats, Inbred Strains; D011960:Receptors, Estrogen / Q000302:isolation & purification*; D014599:Uterus / Q000032:analysis*
D011960:Receptors, Estrogen; D004958:Estradiol; D011189:Potassium Chloride