title: Analysis of fenitrothion and metabolites in stored wheat.
abstract: A simple and rapid method for the analysis of fenitrothion and its metabolites, fenitrooxon, S-methyl fenitrothion, demethyl fenitrothion, demethyl S-methyl fenitrothion, 3-methyl-4-nitrophenol, and dimethyl phosphorothioic acid in stored wheat has been developed. Simultaneous analysis of the extract was conducted using FPD-GLC after derivatization with diazoethane except for 3-methyl-4-nitrophenol which was analyzed directly by EC-GLC. Recoveries of all compounds from wheat fortified at the levels from 0.1 to 5.0 ppm were greater than 90%. The developed method was used to quantitatively determine major metabolites found in grain treated with fenitrothion and stored at 20 degrees C for 12 months. Demethyl fenitrothion, 3-methyl-4-nitrophenol, and dimethyl phosphorothioic acid were the major breakdown products of fenitrothion found in stored wheat. Confirmation of these metabolites was carried out by chemical derivatization plus FPD-GLC and by TLC.
mesh_terms: D001711:Biotransformation; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas / Q000379:methods; D002855:Chromatography, Thin Layer; D005278:Fenitrothion / Q000032:analysis* / Q000378:metabolism; D010573:Pesticide Residues / Q000032:analysis; D013997:Time Factors; D014908:Triticum / Q000032:analysis*
chemical_list: D010573:Pesticide Residues; D005278:Fenitrothion