[Homogeneity of the 30S fraction of nuclear ribonucleoprotein particles. Electrophoresis of intact particles].
A method of electrophoresis of nuclear 30S particles is described. In contrast to the earlier methods based on the fixation of RNP particles with formaldehyde and bifunctional agents with subsequent treatment with sodium dodecylsulphate, the new method treats intact particles in the presence of 1% triton X-100. One of the advantages of the proposed method is the possibility of using the polyacrylamid gel electrophoresis with high resolution capacity in addition to agarose gel electrophoresis. The developed method gives a possibility to estimate the homogeneity and nativity of RNP particles without additional separation by sucrose gradient or purification in nuclear extracts. There is a correlation between the distribution of the particles in the sucrose gradient and the picture of the mobility of these particles in agarose gel.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D005557:Formaldehyde; D008099:Liver / Q000032:analysis; D008970:Molecular Weight; D009698:Nucleoproteins / Q000032:analysis*; D051381:Rats; D012261:Ribonucleoproteins / Q000032:analysis*
D009698:Nucleoproteins; D012261:Ribonucleoproteins; D005557:Formaldehyde
Gomogennost' fraktsii iadernykh ribonukleoproteidnykh 30S chastits. Elektroforez intaktnykh chastits.