Some relatively simple steps toward a computer system for the analysis of two-dimensional gel-electrophoresis autoradiographs.
A computerized system for the quantitative comparison of two-dimensional polyacrylamide gel autoradiographs is being developed that requires relatively limited hardware resources. Two fast, simple, and stable programs--one for background and streak subtraction and one for peak detection--have been developed and tested. Two methods developed by others--one for image smoothing and one for peak matching--also have been tested. A very simple spot-density integration program that works on isolated spots has been written and is being developed further.
D001345:Autoradiography; D003201:Computers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D008722:Methods; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*
D011506:Proteins