Establishment and characterization of human neuroblastoma and ganglioneuroblastoma cell lines.
The establishment in culture and characterization of 4 human neuroblastoma (NB) cell lines and 1 human ganglioneuroblastoma cell line are described. Each cell line fulfilled at least 2 of 4 criteria for malignant or transformed cells: viz., subcultured more than 70 times, high saturation density with absence of contact inhibition, population-doubling time within a range of 10-40 h, and tumour formation in nu/nu mice. Each cell line also fulfilled at least 2 of 3 criteria for neuroblastoma cells: viz., humoral and cell-mediated immune reactivity toward NB-associated cell-surface antigen, intracellular storage and extra-cellular secretion of catecholamines, and characteristic neuroblast and ganglion-cell morphology.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D002395:Catecholamines / Q000096:biosynthesis; D002455:Cell Division; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D005260:Female; D005729:Ganglioneuroma / Q000276:immunology / Q000473:pathology*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron; D009447:Neuroblastoma / Q000276:immunology / Q000473:pathology*
D000951:Antigens, Neoplasm; D002395:Catecholamines