Two-factor requirement for murine megakaryocyte colony formation.
WEHI-3 cell-conditioned medium with the capacity to stimulate megakaryocyte colony formation was separated by Sephadex G-150 column chromatography. The development of colonies containing megakaryocytes was observed only when mixing experiments were performed. Individual fractions did not support megakaryocyte colony growth. The two factors in WEHI-3 CM required for megakaryocyte colony growth had apparent average molecular weights of 35,000 daltons (megakaryocyte CSF) and 100,000 daltons (megakaryocyte potentiator). The results were confirmed in serum-free conditions in which colonies were directly identified in the cultures by acetylcholinesterase staining. Two growth factors may be necessary for the genesis of megakaryocytic colonies.
D000818:Animals; D001853:Bone Marrow / Q000502:physiology; D002460:Cell Line; D003470:Culture Media; D006133:Growth Substances / Q000302:isolation & purification / Q000502:physiology*; D007700:Kinetics; D007942:Leukemia, Experimental / Q000503:physiopathology*; D008533:Megakaryocytes / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL
D003470:Culture Media; D006133:Growth Substances