A technique for assaying the activities of alpha-GPD, LDH, and MDH in the teleost, the goldfish, Carassius auratus.
1. A spectrophotometric assay using rho-iodo-nitro-triphenyl tetrazolium chloride as the indicator was developed for splenic tissue in the goldfish Carassius auratus. 2. This technique permits a rapid test for the activities of the enzymes lactic dehydrogenase, malic dehydrogenase, and alpha-glycerophosphate dehydrogenase which are indicative of the relative activities of various metabolic pathways.
D000818:Animals; D001667:Binding, Competitive; D003530:Cyprinidae / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis*; D006054:Goldfish / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008291:Malate Dehydrogenase / Q000032:analysis*; D009132:Muscles / Q000201:enzymology; D013154:Spleen / Q000201:enzymology; D013696:Temperature
D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase