title: Quantitative analysis of ethynodiol diacetate and ethinyl estradiol/mestranol in oral contraceptive tablets by high-performance liquid chromatography.
abstract: A procedure is described for the assay of ethynodiol diacetate and ethinyl estradiol/mestranol by HPLC using two UV detectors at 210 and 280 nm. The system was acetonitrile 38% (v/v) in water as mobile phase on a 250 x 3.2-mm i.d. RP-2 column, with butylated hydroxytoluene as the internal standard. There was greater than 99% recovery from synthetic preparations and the coefficient of variation was greater than 2.0% for formulations.
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003276:Contraceptives, Oral / Q000032:analysis*; D003277:Contraceptives, Oral, Combined / Q000032:analysis*; D004997:Ethinyl Estradiol / Q000032:analysis*; D005040:Ethynodiol Diacetate / Q000032:analysis*; D008656:Mestranol / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013607:Tablets / Q000032:analysis
chemical_list: D003276:Contraceptives, Oral; D003277:Contraceptives, Oral, Combined; D013607:Tablets; D004997:Ethinyl Estradiol; D005040:Ethynodiol Diacetate; D008656:Mestranol