Peroxisome proliferation in cultured rat hepatocytes produced by clofibrate and phthalate ester metabolites.
Adult rat hepatocytes cultured for 48 h in the presence of 0.2 mM clofibrate, mono-(2-ethylhexyl)-phthalate (MEHP) or 2-ethylhexanol (2-EHA) contained increased numbers of peroxisomes. In keeping with the effects of these compounds in vivo, the peroxisome proliferation was associated with marked increases (up to 15-fold) in the activity of carnitine acetyltransferase. No such effects were produced by n-hexanol or two microsomal enzyme inducers, phenobarbital and 1,2-benzanthracene. These results suggest that cultured hepatocytes may provide a useful model system for studying chemically induced peroxisome proliferation.
D000818:Animals; D002332:Carnitine O-Acetyltransferase / Q000096:biosynthesis; D002478:Cells, Cultured; D002994:Clofibrate / Q000633:toxicity*; D004051:Diethylhexyl Phthalate / Q000378:metabolism / Q000633:toxicity*; D004790:Enzyme Induction; D000441:Hexanols / Q000633:toxicity; D008099:Liver / Q000187:drug effects*; D008297:Male; D008830:Microbodies / Q000187:drug effects*; D009940:Organoids / Q000187:drug effects*; D010795:Phthalic Acids / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains
D000441:Hexanols; D010795:Phthalic Acids; D004051:Diethylhexyl Phthalate; D002332:Carnitine O-Acetyltransferase; D002994:Clofibrate