In-vitro zona-lytic activity in uterine fluid from ovariectomized mice treated with oestradiol-17 beta and progesterone.
Mouse embryos collected before implantation were incubated in vitro for 24 h with fluid rinsed from the uteri of ovariectomized female mice injected with progesterone, oestradiol-17 beta + progesterone, oestradiol-17 beta + progesterone, or oestradiol-17 beta alone. Although none of the zonae was completely dissolved, those incubated in fluid from animals treated with oestradiol + progesterone were subsequently more soluble in sodium thiocyanate (NaSCN) than those incubated similarly in control buffer, indicating a sublytic change during the incubation with uterine washings. Zonae incubated in fluid from animals injected with either hormone alone did not undergo such a change.
D000818:Animals; D001826:Body Fluids / Q000187:drug effects / Q000201:enzymology*; D002369:Castration; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D051379:Mice; D010063:Ovum; D011374:Progesterone / Q000494:pharmacology*; D014599:Uterus / Q000187:drug effects / Q000201:enzymology*; D015044:Zona Pellucida
D011374:Progesterone; D004958:Estradiol