Theoretical and mathematical conditions for use of linearization methods for saturation-type radioassay data.
The algebraic equivalency of nine different methods for the linearization of saturation-type radioassay data was previously demonstrated mathematically to hold under the assumption that the antibody is saturated with ligand. Although compete saturation with antibody never occurs, a good approximation to a saturation condition occurs when the product of the labeled ligand and the affinity constant is 100 or more. The mathematical basis for determining when methods for the linearization of saturation-type radioassay data are valid is derived from a development of the first-order mass action law applied to ligand-antibody reactions.
D000915:Antibody Affinity; D007700:Kinetics; D008962:Models, Theoretical; D011863:Radioimmunoassay / Q000379:methods*; D011869:Radioligand Assay / Q000379:methods*