Two serum pancreatic isoamylase determinations compared.
Serum pancreatic isoamylase activity was measured by a method involving inhibition of salivary isoamylase and by a well-known agarose electrophoretic method, modified by us. We saw changes in electrophoretic patterns of pancreatic isoamylase fractions after storage of serum samples for three weeks at 4-8 degrees C, but with the inhibition method no alterations in activities were found. Within-assay and between-assay imprecisions of both methods were about the same. Serum pancreatic amylase activities as measured by the inhibition method exceeded by about 10% those obtained by the electrophoretic method. The inhibition method seems to be a reasonable candidate for routine application, whereas the electrophoretic method is still time-consuming and requires special skill to perform. Some suggestions are given for improving the calibration of the inhibition method recommended by the manufacturer.
D001793:Blood Preservation; D003080:Cold Temperature; D004587:Electrophoresis, Agar Gel; D006026:Glycoside Hydrolases / Q000097:blood*; D006801:Humans; D007517:Isoamylase / Q000097:blood*; D010179:Pancreas / Q000201:enzymology*; D010182:Pancreatic Diseases / Q000097:blood; D011933:Reagent Kits, Diagnostic; D012016:Reference Values; D012463:Saliva / Q000201:enzymology; D013997:Time Factors
D011933:Reagent Kits, Diagnostic; D006026:Glycoside Hydrolases; D007517:Isoamylase