Glycosyl transferase activity in homogenates of adult Dirofilaria immitis.
Homogenates of adult Dirofilaria immitis possess a microsomal enzyme system able to transfer mannose from GDPmannose to endogenous lipid intermediate(s) and exogenous dolichol monophosphate. A divalent metal was required with Mn2+ being the most effective; other requirements for optimal activity included Triton X-100, EDTA and either ATP or NaF. The maximal rate of mannose transfer to the lipid acceptor by the filarial system, 1.6 pmol.min-1.mg-1 protein, occurred at 37 degrees C and pH 7.0, and this was inhibited 50% by 8 microM diumycin and not at all by 100 microM tunicamycin. D. immitis microsomes also were shown to promote the transfer of mannose to derivatives of alpha-lactalbumin, resulting in the synthesis of a mannose-labeled glycoprotein.
D000818:Animals; D004183:Dirofilaria immitis / Q000201:enzymology*; D004288:Dolichol Phosphates / Q000378:metabolism; D005260:Female; D005370:Filarioidea / Q000201:enzymology*; D006602:Hexosyltransferases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007768:Lactalbumin / Q000378:metabolism; D008345:Manganese / Q000494:pharmacology; D008358:Mannose / Q000378:metabolism; D008364:Mannosyltransferases / Q000378:metabolism*; D008861:Microsomes / Q000201:enzymology; D010743:Phospholipids / Q000494:pharmacology; D013696:Temperature
D004288:Dolichol Phosphates; D010743:Phospholipids; C026602:diumycin; C028306:dolichol monophosphate; D008345:Manganese; D007768:Lactalbumin; D006602:Hexosyltransferases; D008364:Mannosyltransferases; D008358:Mannose