title: Mechanisms of contact photosensitivity in mice: II. Langerhans cells are required for successful induction of contact photosensitivity to TCSA.
abstract: The role of Langerhans cells in the induction of contact photosensitivity (CPS) to tetrachlorosalicylanilide (TCSA) was investigated in mice. CPS was induced by 2 daily paintings of 50 microliters of 1% TCSA in acetone plus black light irradiations for 2.5 hr. Both ears were challenged with 20 microliters of 0.1% TCSA in ethanol plus black light irradiation for 2.5 hr on day 5, and ear thickness was read at 24 hr. The density of LCs detected as ATPase-positive cells dramatically decreased 2 days after exposure to UVB. CPS was not induced by painting the photoallergen to the skin which had been pre-irradiated with UVB. The ear swelling response returned to the normal level when the mice were sensitized 12 days after UVB exposure in accordance with the complete regeneration of ATPase-positive cells. Dose of UVB in the present study did not affect the development of CPS through systemic mechanism. These demonstrations indicate that LCs play an important role in the induction of CPS.
mesh_terms: D000818:Animals; D003877:Dermatitis, Contact / Q000209:etiology*; D005260:Female; D007801:Langerhans Cells / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008813:Mice, Inbred ICR; D010787:Photosensitivity Disorders / Q000209:etiology*; D012457:Salicylamides / Q000494:pharmacology*; D012458:Salicylanilides / Q000276:immunology / Q000494:pharmacology*; D014466:Ultraviolet Rays
chemical_list: D012457:Salicylamides; D012458:Salicylanilides; C003390:3,3',4',5-tetrachlorosalicylanilide