Interference of immunoglobulins in two glucagon radioimmunoassays.
Radioimmunoassays of glucagon in plasma may be complicated by interaction with other substances of high molecular mass. Precipitates of such substances with ammonium sulfate showed, after isoelectric focusing, two fractions having glucagon immunoreactivity. One fraction (pl approximately 10) evidently is associated with the Fc portion (but not the Fab portion) of purified polyclonal immunoglobulin G (IgG). Equal amounts of purified monoclonal IgG of various subclasses, especially IgG 1, gave different "glucagon" readings, suggesting that some IgG may interfere more strongly than others. The other fraction (pI 5-6) appeared less consistently, and on gel chromatography appeared to be slightly larger than IgG. Together these fractions add about 50-100 ng/L to the immunoreactive glucagon values in plasma. Therefore methods in which glucagon is extracted before assay should be used for determining the concentration of glucagon present physiologically.
D000645:Ammonium Sulfate; D002850:Chromatography, Gel; D003429:Cross Reactions; D005189:False Positive Reactions; D005934:Glucagon / Q000097:blood*; D006801:Humans; D007074:Immunoglobulin G; D007525:Isoelectric Focusing; D008970:Molecular Weight; D011863:Radioimmunoassay
D007074:Immunoglobulin G; D005934:Glucagon; D000645:Ammonium Sulfate