A long-lived thymidine pool in epithelial stem cells.
The labelling index (LI) of the individual basal cell positions of the anterior column of mouse tongue filiform papillae was assessed with time after an injection of [3H]TdR at 12.00 hours (the minimum point in the circadian LI rhythm). An initial doubling of the LI in the stem cell zone due to cell division was followed by a second rise of 14-16% 16 hr after injection and this occurred even in the presence of vincristine. Although the uptake of [3H]TdR and the initial LI doubling were largely prevented by a preceding injection of hydroxyurea, the 14-16% LI rise was still observed. The possible explanations are discussed, the favoured one being that an average of one of the six or seven cells (the stem cell) in each stem cell zone can store [3H]TdR in a long-lived precursor pool for at least 16 hr before being utilized for DNA synthesis. This complements previously published work which suggested that one cell in each stem cell zone may selectively segregate DNA at mitosis.
D000818:Animals; D001345:Autoradiography; D002455:Cell Division / Q000187:drug effects; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism*; D005260:Female; D006918:Hydroxyurea / Q000494:pharmacology; D008297:Male; D051379:Mice; D013936:Thymidine / Q000378:metabolism*; D014059:Tongue; D014750:Vincristine / Q000494:pharmacology
D014750:Vincristine; D013936:Thymidine; D006918:Hydroxyurea