Secretin enhances [14C]erythritol clearance in unanesthetized dogs.
To determine the effect of secretin infusion on clearance of inert markers into bile, unanesthetized dogs fitted with Thomas cannulas received continuous infusions of [14C]erythritol and [3H]inulin throughout study. Taurocholic acid administered sequentially at 9.0, 20.0, and 40.0 mumol/min enhanced [14C]erythritol clearance, and GIH secretin (3 units/min) administered along with TCA (40.0 mumol/min) increased [14C]erythritol clearance from 4.9 +/- 1.2 ml/10 min to 6.8 +/- 1.3 ml/10 min (P less than 0.001), but simultaneously measured [3H]inulin clearance was unaltered. Secretin alone also increased [14C]erythritol clearance but did not alter [3H]inulin clearance. The increase in [14C]erythritol clearance per unit increase in bile flow was less during secretin infusion than TCA. Thus, secretin increases [14C]erythritol transport through restricted channels, probably distal to the canaliculi. [14C]Erythritol may not be an accurate marker for canalicular bile flow in dogs during secretin infusion.
D000818:Animals; D001646:Bile / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002250:Carbon Radioisotopes; D004285:Dogs; D004896:Erythritol / Q000378:metabolism*; D005260:Female; D007444:Inulin / Q000378:metabolism*; D008297:Male; D012633:Secretin / Q000008:administration & dosage*; D013656:Taurocholic Acid / Q000008:administration & dosage*
D002250:Carbon Radioisotopes; D012633:Secretin; D013656:Taurocholic Acid; D007444:Inulin; D004896:Erythritol