Characterisation of the chick brain high molecular weight multiply phosphorylated microtubule associated protein.
A high-molecular-weight microtubule-associated protein from chick brain, which has previously been shown to be phosphorylated in vitro to the extent of 10-12 mol X mol-1, is shown to have the same properties as reported for the microtubule-associated protein MAP2 from calf and porcine brain in terms of its molecular weight, sensitivity to trypsin and alpha-chymotrypsin, the molecular weights of the primary proteolytic degradation products, and the failure of certain of the latter components to bind to assembled microtubules. The primary 260,000-Mr tryptic product can be phosphorylated to at least 4 mol X mol-1, in contrast to an earlier report with calf brain MAP2 which noted a single phosphorylation.
D000818:Animals; D001923:Brain Chemistry; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D066298:In Vitro Techniques; D008869:Microtubule-Associated Proteins; D008970:Molecular Weight; D009218:Myosins / Q000302:isolation & purification; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010766:Phosphorylation; D011506:Proteins / Q000032:analysis*; D014357:Trypsin
D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D014357:Trypsin; D009218:Myosins