Permeability of thermally damaged skin v: permeability over the course of maturation of a deep partial-thickness wound.
The permeability of eschar is an important factor governing rational approaches to topical control of burn wound sepsis. Previous work has shown the burn wound to have a highly variable permeability immediately after burning depending on the manner of burning. But the burn is also a dynamic wound and its physical state changes during the process of maturation. The present studies are an early attempt to characterize wound permeability as a function of maturation. Hairless mice were burned dorsally for 15 seconds on a metal surface maintained at 80 degree C. The time and temperature conditions were chosen to effect a deep partial-thickness to full-thickness injury on the animal. Mice were sacrificed daily post burn over a 2-week period and the permeabilities of 3H-methanol and 14C-butanol through the excised eschar were measured. Te eschar permeability coefficients were directly compared to permeability coefficients for the same compounds found with abdominal skin sections taken concurrently from each animal. It was observed that the branding initially caused a 50 per cent increase in the permeability of methanol and a 300-400 per cent increase in the permeability of maturation. Thereafter permeabilities tended to increase, gradually at first, but accelerating to a maximum which was observed at approximately 10 days. At the maximum, methanol's permeability was 20 times and butanol's 12 times their normal values. For both compounds permeability of eschar decreased past the maximum until termination of the studies at 14 days.
D000287:Administration, Topical; D000818:Animals; D002056:Burns / Q000503:physiopathology*; D002921:Cicatrix; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology; D000432:Methanol / Q000008:administration & dosage / Q000494:pharmacology; D051379:Mice; D008812:Mice, Hairless; D008954:Models, Biological; D010539:Permeability; D012867:Skin / Q000293:injuries / Q000503:physiopathology*; D012869:Skin Absorption; D014945:Wound Healing / Q000187:drug effects
D000431:Ethanol; D000432:Methanol