An automated HPLC method for the assay of propranolol and its basic metabolites in plasma and urine.
An automated HPLC method is described for the simultaneous determination of propranolol, 4-hydroxypropranolol, and N-desisopropylpropranolol in plasma and urine before and after beta-glucuronidase/aryl sulfatase treatment. It involves extraction with ether at pH 10 in the presence of ascorbic acid, added to prevent oxidation of 4-hydroxypropranolol. The compounds are then back extracted into dilute acid and assayed on an HPLC using a fluorescence detector. Three HPLC columns have been used (a phenyl, an octyl, and an octadecyl column). The last column was found to be most reproducible with minimal intercolumn variation. The solvent system includes a combination of acetonitrile, methanol, and phosphoric acid. Concentrations as low as 0.2, 1.0, and 0.2 ng/ml of propranolol, 4-hydroxypropranolol, and N-desisopropylpropranolol, respectively, can be measured using 1 ml of plasma.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D011433:Propranolol / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000652:urine
C012878:N-desisopropylpropranolol; C001422:4-hydroxypropranolol; D011433:Propranolol