title: Features of small intestinal pathology (epithelial cell kinetics, intraepithelial lymphocytes, disaccharidases) in a primary Giardia muris infection.
abstract: In an attempt to correlate host and parasite-related events occurring during the course of a primary Giardia infection in the mouse we have measured epithelial cell kinetics, enzymes, and intraepithelial lymphocytes at different stages of the infection. New methods were developed for the accurate measurement of parasite numbers and distribution within the gut. In jejunum a modest decrease in villus length and intraepithelial lymphocytes at week 1 preceded a pronounced disaccharidase deficiency at week 2, the time of maximum trophozoite numbers, whereas crypt lengthening and increased cell production became maximal at week 3. As trophozoite numbers fell the intraepithelial lymphocyte count and disaccharidase values rose. With the exception of the intraepithelial lymphocyte count, which followed the same pattern as in jejunum but two weeks later, the changes seen in the ileum were the opposite of those in jejunum, suggesting rapid ileal adaptation. The results indicate that the disaccharidase deficiency associated with giardiasis is likely to represent a direct effect of the parasite on the brush border rather than enterocyte immaturity, whereas the intraepithelial lymphocyte response reflects host immunity to the parasite. Profound adaptive changes occur throughout the small intestine in response to a relatively localised insult.
mesh_terms: D000818:Animals; D002455:Cell Division; D004186:Disaccharidases / Q000032:analysis; D004848:Epithelium / Q000473:pathology; D005260:Female; D005873:Giardiasis / Q000473:pathology*; D007082:Ileum / Q000201:enzymology / Q000473:pathology; D007421:Intestine, Small / Q000473:pathology*; D007583:Jejunum / Q000201:enzymology / Q000473:pathology; D007958:Leukocyte Count; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA
chemical_list: D004186:Disaccharidases