Action of peptidolipidic antibiotics of the iturin group on erythrocytes. Effect of some lipids on hemolysis.
Iturin A, bacillomycin L and bacillomycin L dimethyl ester have a strong lytic activity upon human erythrocytes while iturin C is totally inactive. The hemolytic action of the antibiotics is inhibited by free cholesterol as well as by cholesterol included in mixed liposomes of phosphatidylcholine-cholesterol and to a lesser extent by phosphatidylcholine liposomes. This inhibition is the result of an interaction between the antibiotic and added lipids which diminishes the concentration of free antibiotic available to lyse erythrocytes. The inhibitory effect of liposomes on hemolysis demonstrated the affinity of the antibiotic for artificial membrane, especially those containing cholesterol.
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents / Q000494:pharmacology*; D002784:Cholesterol / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000648:ultrastructure*; D004912:Erythrocytes / Q000648:ultrastructure*; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D010455:Peptides; D010456:Peptides, Cyclic / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D010455:Peptides; D010456:Peptides, Cyclic; C013579:iturin A; C033468:iturin C; C015015:bacillomycin L; C033467:bacillomycin L dimethyl ester; D002784:Cholesterol