A liquid culture system for murine megakaryocyte progenitor cells.
A liquid culture system is described for murine megakaryocyte progenitor cells (CFU-M) in the presence of pokeweed-mitogen-stimulated spleen-cell conditioned medium. There were dose-related responses between the number of CFU-M developed and the number of cells cultured and the dosage of conditioned medium in this liquid culture system. Murine CFU-M were abundantly cloned in this system an the plating efficiency was similar in comparison with that in a plasma clot system. The acetylcholinesterase-positive colonies (more than 4 acetylcholinesterase-positive cells) were clearly seen on day 3 of culture, and they reached a maximum (60.5 +/- 10.7/2 x 10(5) cells) on day 7 of culture. Ultrastructural analyses of megakaryocytic maturation in this system showed that a few megakaryocytes produced platelets that were released in the culture medium on day 5 of culture. This liquid culture system is suitable for the study of the dynamic process of the megakaryocyte-platelet system.
D000818:Animals; D001792:Blood Platelets / Q000166:cytology / Q000648:ultrastructure; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D008533:Megakaryocytes / Q000166:cytology* / Q000648:ultrastructure; D051379:Mice
D003470:Culture Media