Studies on the endogenous phosphatides of mammalian pancreas and their hydrolysis by endogenous phospholipases - I. The lipids of dog pancreas and their in vitro hydrolysis, primed by trypsin, by phospholipase A2.
1. The phosphoglyceride and sphingolipid content of dog pancreas has been determined and their in vitro response to the endogenous lipolytic enzymes, initiated by trypsin, at pH 7.4 was examined by TLC technology. 2. The glyceryl ether phosphatides were found to consist primarily of PE and PC, each with alkyl and alk-1-enyl components both of which were hydrolyzed to their respective 1-radyl lyso derivatives under the described incubation conditions. 3. A new TLC visualizing reaction for lipids, OSPAS, is described.
D000818:Animals; D004285:Dogs; D005260:Female; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D050356:Lipid Metabolism; D008244:Lysophosphatidylcholines / Q000378:metabolism; D008297:Male; D010179:Pancreas / Q000201:enzymology / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D014357:Trypsin / Q000378:metabolism*
D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D014357:Trypsin