Cytotoxicity and mutagenicity of dinitrotoluenes in Chinese hamster ovary cells.
Technical grade dinitrotoluene (DNT), a mixture composed predominantly of 2,4- and 2,6-DNT but containing lesser amounts of 2,3-, 2,5-, 3,4- and 3,5-DNT, has been shown to be a hepatocarcinogen in rats, The mutagenicity of these compounds has been evaluated using the CHO/HGPRT system, a quantitative mammalian somatic cell mutational assay. Dinitrotoluenes were tested for their ability to induce mutation to 6-thioguanine (TG) resistance in the presence and absence of microsomal preparations (PMS) from rats pretreated with the mixed function oxidase inducer Aroclor 1254. A marked difference in cytotoxicity of the isomers was observed. However, neither technical grade DNT nor any of the purified isomers resulted in a significant increase in the TG-resistant fraction of surviving cells, with or without added PMS.
D000818:Animals; D001711:Biotransformation; D002273:Carcinogens / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004136:Dinitrobenzenes / Q000494:pharmacology*; D004351:Drug Resistance; D005260:Female; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens; D009154:Mutation; D009578:Nitrobenzenes / Q000494:pharmacology*; D010053:Ovary; D051381:Rats; D011916:Rats, Inbred F344; D013329:Structure-Activity Relationship; D013866:Thioguanine / Q000494:pharmacology
D002273:Carcinogens; D004136:Dinitrobenzenes; D009153:Mutagens; D009578:Nitrobenzenes; C016403:2,4-dinitrotoluene; D013866:Thioguanine; C023514:2,6-dinitrotoluene