Prostatic acid phosphatase, purification and iodination using Iodogen.
Prostatic acid phosphatase was purified from prostatic adenomas. The procedure involved chromatography on Concanavalin A-Sepharose, DEAE-cellulose, Bio-Gel P-150 and L-tartrate-Sepharose. The purified phosphatase hydrolyzed p-nitrophenyl phosphate at a rate of 270 mumol . mg-1 . min-1 (25 degrees C) and showed homogeneity upon polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The final prostatic acid phosphatase preparation was pure and the antisera were monospecific as judged by the highly sensitive technique of crossed immunoelectrophoresis. Of the procedures evaluated for the iodination of the purified enzyme, oxidation with Iodogen was found to give the best iodinated product.
D000135:Acid Phosphatase / Q000302:isolation & purification* / Q000378:metabolism; D000236:Adenoma / Q000032:analysis*; D002845:Chromatography; D004355:Drug Stability; D006801:Humans; D007093:Imidazoles; D007123:Immunoelectrophoresis, Two-Dimensional; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D008297:Male; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011471:Prostatic Neoplasms / Q000032:analysis*; D014508:Urea / Q000031:analogs & derivatives*
D007093:Imidazoles; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; C012385:1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril; D014508:Urea; D000135:Acid Phosphatase