Rapid colorimetric assay for acetaminophen without salicylate or phenylephrine interference.
A simple rapid, and economical procedure is presented for the quantitative determination of acetaminophen in serum. Acetaminophen is extracted into solvent, and, when back-extracted into base, is simultaneously reacted with Folin-Ciocalteau reagent to form a colored complex. The potential interference of bilirubin is eliminated by treatment with p-diazobenzene sulfonic acid. Results obtained with this method agree with those obtained by "high-pressure" liquid chromatography.
D000082:Acetaminophen / Q000097:blood* / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D003979:Diazonium Compounds; D005189:False Positive Reactions; D006801:Humans; D012016:Reference Values; D019966:Substance-Related Disorders; D013425:Sulfanilic Acids / Q000031:analogs & derivatives
D003979:Diazonium Compounds; D013425:Sulfanilic Acids; D000082:Acetaminophen; C000469:diazobenzenesulfonic acid