Determination of reduced biopterins by high pressure liquid chromatography and subsequent electrochemical detection.
L-erythro-Tetrahydrobiopterin plays an important role as the cofactor of various hydroxylation reactions. We report here the separation of biopterin and its reduced forms by reversed-phase high pressure liquid chromatography. Subsequent electrochemical detection yields a sensitivity at the picomole level for dihydrobiopterin and tetrahydrobiopterin.
D001708:Biopterins / Q000031:analogs & derivatives / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D007202:Indicators and Reagents; D011621:Pteridines / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D011621:Pteridines; D001708:Biopterins; C017226:7,8-dihydrobiopterin; C003402:sapropterin