Neutrophil chemotaxis by Propionibacterium acnes lipase and its inhibition.
The chemoattraction of Propionibacterium acnes lipase for neutrophils and the effect of lipase inhibitor and two antibiotic agents on the chemotaxis were evaluated. Of the various fractions tested, partially purified lipase (fraction 2c) was the most active cytotaxin produced by P. acnes. Serum mediators were not required for the generation of chemotaxis by lipase in vitro. Diisopropyl phosphofluoridate at low concentration (10(-4) mM) completely inhibited lipase activity as well as polymorphonuclear leukocyte chemotaxis generated by lipase. Tetracycline hydrochloride and erythromycin base at concentrations of 10(-1) mM and 1 mM, respectively, caused 100% inhibition of PMN migration toward lipase or zymosan-activated serum. The inhibiting activity of the antibiotics was directed against cells independently of any effect on lipase. Chemotaxis by P. acnes lipase suggests a wider role for this enzyme in the inflammatory process and the pathogenesis of acne vulgaris.
D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004917:Erythromycin / Q000494:pharmacology; D006801:Humans; D007531:Isoflurophate / Q000494:pharmacology; D008049:Lipase / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D009504:Neutrophils / Q000502:physiology; D011425:Propionibacterium acnes / Q000201:enzymology*; D013752:Tetracycline / Q000494:pharmacology
D007531:Isoflurophate; D004917:Erythromycin; D008049:Lipase; D013752:Tetracycline