[Isolation, purification and properties of acid deoxyribonuclease from silkworm Bombyx mori eggs].
The acid deoxyribonuclease was isolated from Bombyx mori eggs and its physico-chemical properties were investigated. The enzyme purified 160-fold did not contain admixtures of phosphomono- and phosphodiesterases or ribonuclease. The molecular weight of the enzyme is 40 000 +/- 1000, isoelectric point lies at 6.5. The maximum activity is revealed at pH 5.2, 50 degrees. The DNAase is insignificantly activated by Mg2+ and is inhibited by Cu2+ and Zn2+. The enzyme preferentially hydrolyzes native DNA and is an endonuclease splitting DNA down to 5'-oligonucleotides.
D000818:Animals; D012831:Bombyx / Q000201:enzymology; D002413:Cations, Divalent; D003851:Deoxyribonucleases / Q000302:isolation & purification* / Q000378:metabolism; D005260:Female; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D010063:Ovum / Q000201:enzymology*
D002413:Cations, Divalent; D003851:Deoxyribonucleases
Vydelenie, ochistka i svoĭstva kisloĭ dezoksiribonuckleazy greny tutovogo shelkopriada Bombyx mori.