Some properties of porcine carboxypeptidase N.
Some properties of carboxypeptidase N purified from pig serum have been investigated. The amino acid composition resembles that of human carboxypeptidase N, but differs markedly from that of porcine carboxypeptidase B. The enzyme contains a mass fraction of 0.1 carbohydrates. Both ester and peptide substrates are hydrolyzed at the same site. Peptide substrates are hydrolyzed if lysine or arginine is the C-terminal amino acid, and provided that the penultimate amino acid is not proline. Argininic acid and epsilon-aminocaproic acid are good inhibitors of the enzyme.
D000596:Amino Acids / Q000097:blood; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002268:Carboxypeptidases / Q000097:blood*; D007700:Kinetics; D001121:Lysine Carboxypeptidase / Q000097:blood* / Q000302:isolation & purification; D013379:Substrate Specificity; D013552:Swine
D000596:Amino Acids; D002241:Carbohydrates; D002268:Carboxypeptidases; D001121:Lysine Carboxypeptidase