[Influenza virus penetration into the cells of a continuous dog-kidney line].
The penetration of the influenza virus (WSN) into MDCK cells and its intracellular fate was studied using electron microscopy of ultrathin sections of infected cells at early steps of infection, and biochemical analysis of intracellular subviral components. In electron micrographs, the virus particles absorbed on the cells and located inside phagosomes were detected. In both the cases, the fusion of the virus with cell envelopes was distinctly seen. The fusion took place with incubation at 37 degrees C and 4 degrees C, thus suggesting a non-enzymatic mechanism. The inner virus component released into the cytosol after fusion represented virus cores, i. e. ribonucleoproteins (the final products of uncoating) surrounded by the inner protein membrane consisting of matrix (M) protein. The cores were accumulated in perinuclear cytoplasm of infected cells and were most probably intermediates in virus uncoating.
D000327:Adsorption; D000818:Animals; D002460:Cell Line; D004285:Dogs; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure; D007668:Kidney; D008854:Microscopy, Electron; D009975:Orthomyxoviridae / Q000472:pathogenicity* / Q000648:ultrastructure; D009976:Orthomyxoviridae Infections / Q000382:microbiology*; D014771:Virion / Q000648:ultrastructure; D014776:Virus Cultivation
Proniknovenie virusa grippa v kletki perevivaemoi linii pochek sobak.