title: The effects of hypoxia on human skin, lung and tendon cells in vitro.
abstract: Fibroblasts derived from human foetal tendon, skin and lung were cultured in media containing dissolved oxygen at concentrations of 20%, 10% and 2.7%. Total protein and collagen synthesis were reduced at 2.7% oxygen, tendon-derived cells proving significantly less sensitive than lung and skin fibroblasts. The effects of hypoxia were more marked in low-density cultures than in confluent cultures. Proliferation rates varied greatly between the 3 cell lines but were not affected by hypoxia. The implications of these results with respect to tendon degeneration and rupture are discussed.
mesh_terms: D002455:Cell Division; D002460:Cell Line; D003094:Collagen / Q000096:biosynthesis; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000378:metabolism*; D010101:Oxygen Consumption; D014176:Protein Biosynthesis; D012867:Skin / Q000378:metabolism*; D013710:Tendons / Q000378:metabolism*
chemical_list: D003094:Collagen