Comparison of radioimmunoassay, chemical assay (HPLC) and bioassay for arginine vasopressin in synthetic standards and posterior pituitary tissue.
Radioimmunoassay and chemical assay (high performance liquid chromatography (HPLC) with fluorescence detection) for arginine vasopressin (AVP) in purified synthetic preparations and in posterior lobe tissue samples (ox and rat) yielded essentially identical values. In tissue samples from the neurohypophysis, the reproducibility of the former method was roughly twice as good. The routinely used blood pressure bioassay was very poorly reproducible and yielded values deviating from the two ther methods. In all three assays, the coefficient of variation was roughly five times higher in tissue samples than in synthetic preparations. It is suggested that a chemical rather than a biological assay is used for standardization of AVP for radioimmunoassay. Large differences were found in the quality of AVP preparations potentially usable as standards and tracers (after iodination) for radioimmunoassay. All commercial preparations should therefore be checked for their AVP and impurity content by high performance liquid chromatography.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis*; D001794:Blood Pressure; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D010904:Pituitary Gland, Posterior / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats
D000596:Amino Acids; D001127:Arginine Vasopressin