Myocardial injury: quantitation by cell sorting initiated with antimyosin fluorescent spheres.
Spheres coated with antibodies specific for myosin were used to detect myocardial cell membrane disruption by scanning electron microscopy. Injury in a population of cultured myocytes as then followed and measured by fluorescence-activated cell sorting. This approach provides a unique method for quantitating the evolution of myocardial injury and potentially for assessing the efficacy of interventions aimed at myocardial protection.
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D002469:Cell Separation; D003327:Coronary Disease / Q000473:pathology; D003470:Culture Media; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005947:Glucose / Q000494:pharmacology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009206:Myocardium / Q000473:pathology*; D009218:Myosins / Q000032:analysis* / Q000276:immunology
D003470:Culture Media; D009218:Myosins; D005947:Glucose