title: Development of sensitivity to acetylcholine in cultured chick embryo sympathetic ganglion neurones.
abstract: Dissociated sympathetic neurones from chick embryos of various ages were maintained in culture for several days and changes in sensitivity to iontophoretically applied acetylcholine (ACh) measured over 5 days in vitro. Neurones from 12-day embryos show a marked increase in ACh sensitivity, neurons from 14-day embryos a smaller change and those from 19-day embryos do not alter. These changes parallel those observed previously for binding of [125I]alpha-bungarotoxin.
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000367:Age Factors; D000818:Animals; D002038:Bungarotoxins / Q000494:pharmacology; D002478:Cells, Cultured; D002642:Chick Embryo; D005728:Ganglia, Sympathetic / Q000187:drug effects*; D007478:Iontophoresis; D011950:Receptors, Cholinergic / Q000032:analysis; D011978:Receptors, Nicotinic / Q000032:analysis; D064569:alpha7 Nicotinic Acetylcholine Receptor
chemical_list: D002038:Bungarotoxins; D011950:Receptors, Cholinergic; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor; D000109:Acetylcholine