[Interaction of fluorescent probes with membranes of sarcoplasmic reticulum in AMP deamination].
It is shown that during deamination of AMP with membrane-bound AMP-aminohydrolase of sarcoplasmic reticulum, which is accompanied by the pH increase, there occurs a fluorescence quenching of 1-anilino-8-naphthalinsulphonate and 3-3(1)-dipropylthio-dicarbocyanine bound to membranes. Such a direction in the fluorescence changes is observed when pH is artificially increased from 6.5 to 8.5. No changes are marked in fluorescence of the applied probes in experiments with the use of the sarcoplasmic reticulum fragment vesicles with AMP-aminohydrolase only or in deamination of AMP in a strongly buffered medium as well as under the effect of IMP or NH4Cl in concentrations up to 10 mM. The studies conducted show that the charge of sarcoplasmic reticulum membranes may change in the process of amp deamination.
D000659:AMP Deaminase / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000378:metabolism*; D000817:Anilino Naphthalenesulfonates / Q000494:pharmacology*; D000818:Animals; D052160:Benzothiazoles; D002232:Carbocyanines / Q000494:pharmacology*; D005456:Fluorescent Dyes / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D009709:Nucleotide Deaminases / Q000378:metabolism*; D011804:Quinolines / Q000494:pharmacology*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D000817:Anilino Naphthalenesulfonates; D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D011804:Quinolines; D000249:Adenosine Monophosphate; C012944:3,3'-dipropyl-2,2'-thiadicarbocyanine; C027132:1-anilino-8-naphthalenesulfonate; D009709:Nucleotide Deaminases; D000659:AMP Deaminase
Vzaimodeĭstvie fluorestsentnykh zondov s membranami sarkoplazmaticheskogo retikuluma pri dezaminirovanii AMP.