Simplified procedure for the analysis of 3- and 4-hydroxyproline.
A column chromatographic analysis for 3-hydroxyproline (3-Hyp), 4-hydroxyproline (4-Hyp), and gamma-carboxyglutamic acid (Gla) is described. The analyses of urine and plasma were performed with a JLC-6AH amino acid analyzer. A 0.15 M sodium citrate buffer, pH 2.1, was used for elution. Urinary Gla, 3-Hyp, and 4-Hyp were among the seventeen peaks eluted before aspartic acid. Hyp, Gla, glutamine, and asparagine in plasma were separated by elution with 0.2 M sodium citrate buffer, pH 3.25, containing 10% methanol. This single-column procedure achieves the sequential separation and quantitation of Gla, 3-Hyp, and 4-Hyp in urine as well as plasma, and is applicable to the diagnosis of collagen metabolism disorders.
D001322:Autoanalysis; D002845:Chromatography / Q000379:methods; D003095:Collagen Diseases / Q000097:blood* / Q000652:urine; D006801:Humans; D006909:Hydroxyproline / Q000097:blood* / Q000652:urine
C026215:3-hydroxyproline; D006909:Hydroxyproline