[Rapid method for the determination of urinary oxalic acid by gas liquid chromatography (author's transl)].
A rapid method for the determination of urinary oxalic acid by gas-liquid chromatography is described. The procedure involves extraction of oxalate from urine by tetrahydrofuran followed by evaporation to dryness and subsequent with diesterification with the boron-trifluoride propanol. The derivative is extracted with hexane and is detected by FID gas chromatography. Malonic acid is used as internal standard. Analytical recovery ranged from 94 to 105%. The coefficient of variation in replicate aliquots over the entire range is less than 6%. The expected range for our method is calculated to be 44 to 577 mumol of oxalate per 24-h urine.
D002849:Chromatography, Gas; D005410:Flame Ionization; D006801:Humans; D010070:Oxalates / Q000652:urine*; D012016:Reference Values
D010070:Oxalates
Méthode rapide de dosage de l'acide oxalique urinaire par chromatographie en phase gazeuse.