title: Muscle glycogen storage and its relationship with water.
abstract: This study examined the relationship between muscle glycogen and muscle water content. Exercise dietary manipulations were used to vary skeletal muscle glycogen levels in four groups of rodents: (1) eight animals were sedentary controls (SC); (2) ten animals were treadmill familiarized and allowed to recover 24 h before sacrifice (F); (3) ten animals were treadmill familiarized and exercised to exhaustion (E); (4) ten animals were treadmill familiarized, exercised to exhaustion, and allowed to recover with food and water ad libitum for 72 h (ER). All animals were sacrificed in a resting state to normalize intracellular, extracellular, and interstitial water compartments; thus, the E group was sacrificed 45 m in following their run. The treatments altered skeletal muscle glycogen to values ranging from 10.0 to 30.2 mumol glucosyl units/g wet tissue weight. Neither muscle triglyceride nor protein levels were affected by the treatments. Muscle water content expressed as mumol H2O lost/g wet tissue weight or made relative to protein content showed no consistent relationship to the glycogen content. These data, therefore, do not support the commonly accepted muscle glycogen-to-water ratio of 1.0:2.7 (g:g). Further work is necessary to quantify the exact amount of water that is actually associated with the glycogen complex.
mesh_terms: D000818:Animals; D001834:Body Water / Q000032:analysis*; D004032:Diet; D005110:Extracellular Space / Q000032:analysis; D006003:Glycogen / Q000032:analysis* / Q000378:metabolism; D008297:Male; D009124:Muscle Proteins / Q000032:analysis; D009132:Muscles / Q000032:analysis*; D010805:Physical Conditioning, Animal; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000032:analysis
chemical_list: D009124:Muscle Proteins; D014280:Triglycerides; D006003:Glycogen