[Nonhistone proteins of the rat liver in malignant transformation by nitrosodiethylamine].
Qualitative and quantitative content of nonhistone chromosomal proteins (NHP) from normal and nitrosodiethylamine-transformed rat liver was studied. An increase in the protein/DNA ratio in tumor chromatin, as compared with that of normal tissue, was established. NHP were extracted from the chromatin with 0.4 N N2SO4 and fractionated with two-dimensional polyacrylamide gel electrophoresis. Coomassie staining revealed more than 80 fractions with molecular weights ranging 20-150 th. Some quantitative and qualitative differences in the patterns of NHP from normal and neoplastic tissues were observed. A conclusion is drawn that these differences may be related to structural and/or enzymatic components of NHP.
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004273:DNA, Neoplasm / Q000378:metabolism; D004052:Diethylnitrosamine / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism*; D009602:Nitrosamines / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism*; D051381:Rats
D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D009602:Nitrosamines; D011506:Proteins; D004052:Diethylnitrosamine; D004247:DNA
Negistonovye belki pecheni krysy pri zlokachestvennoĭ transformatsii nitrozodiétilaminom.