Muscarinic acetylcholine receptors on culture glia cells.
Muscarinic acetylcholine receptors (76 fmol/mg protein) were detected on cultured glia cells (astroblasts) from embryonic chicken brain by specific [3H]quinuclidinylbenzilate (QNB) binding at physiological conditions. The QNB binding (Kd = 9.5 x 10(-11)) to the intact cells seems to be cooperative (nH = 1.98) as shown by graphical methods.
D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D007700:Kinetics; D009457:Neuroglia / Q000378:metabolism*; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000378:metabolism*
D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate