[Role of SH-groups in the pharmacology of the action of novurite and ethacrynic acid].
In experiments in vivo the mercuric diuretic, novurite, and ethacrynic acid in therapeutic doses (25 and 50 mg/kg, respectively) do not block the respiration and active transport of calcium in the kidney mitochondria. The diuretic effect of these substances is not prevented by cystein (50 mg/kg), the donator of sulphydryl groups. In vitro novurite (3 mM) and ethacrynic acid (1 mM) block the systems of ionic transport inthe kidney epithelium cells and in concentrations of 0.2 and 0.5 mM, respectively, disturb the oxygen uptake and oxidative phosphorylation in the kidney mitochondria. A preliminary administration of cystein (5 mM) into the incubation medium prevents these effects. Novurite and ethacrynic acid manifest the typical thyol toxins of general cell effect in doses considerably exceeding the therapeutic ones. A specific "kidneys" effect of these substances is not connected with their ability to block SH-group of biologically active substances.
D000479:Alkylmercury Compounds / Q000494:pharmacology*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D004338:Drug Combinations / Q000494:pharmacology; D004976:Ethacrynic Acid / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009941:Organomercury Compounds / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects*; D051381:Rats; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013806:Theophylline / Q000494:pharmacology*
D000479:Alkylmercury Compounds; D004338:Drug Combinations; D009941:Organomercury Compounds; D013438:Sulfhydryl Compounds; C021054:mercurophylline; D013806:Theophylline; D004976:Ethacrynic Acid; D002118:Calcium
Rol' SH-grupp v realizatsii éffektov novurita i ítakrinovoĭ kisloty.