Entrance of cholera enterotoxin subunits into cells.
Quantitative analysis of the staining of cholera enterotoxin on the surface of cells with specific antibodies against each subunit of cholera toxin, using a Fluorescence-Activated Cell Sorter, showed that not only subunit A but also subunit B penetrates the cell membrane. The detection of each subunit inside the cell was facilitated by the use of saponin, an agent which increases membrane permeability.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D002469:Cell Separation; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000378:metabolism*; D005434:Flow Cytometry; D051379:Mice; D010873:Pinocytosis; D012503:Saponins / Q000494:pharmacology; D013950:Thymus Gland
D012503:Saponins; D002772:Cholera Toxin