Spermatogenesis and sperm-specific phosphoglycerate kinase B and lactate dehydrogenase C4 isoenzymes in sex-reversed mice.
The activities of phosphoglycerate kinase B and lactate dehydrogenase C4 were correlated with the state of spermatogenesis in testes of sex-reversed (Sxr) mice. Both isoenzymes were present in Sxr/+,XO testes, which contained late spermatids. LDH-C4 was detected in testes from 5 of 9 Sxr/+,XX mice, but PGK-B was present in testes of only 1 of the 5 mice. Pachytene spermatocytes were the predominant cell types in those Sxr/+,XX testes that contained spermatogenic cells. These observations confirm that PGK-B is restricted to spermatogenic cells and is not present in somatic testicular cells. Furthermore, they suggest that PGK-B synthesis may be initiated later than pachytene, when synthesis of the LDH-C polypeptide is known to commence.
D000818:Animals; D007527:Isoenzymes / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010735:Phosphoglycerate Kinase / Q000378:metabolism*; D013091:Spermatogenesis; D013094:Spermatozoa / Q000201:enzymology; D013737:Testis / Q000473:pathology
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D010735:Phosphoglycerate Kinase