Immobilized ferredoxin-NADP+ reductase: preparation and properties.
Immobilized ferredoxin-NADP+ reductase (FNR) was prepared by coupling reaction of CNBr-Sepharose 4B with the spinach enzyme. The immobilized FNR was found to retain the activity of complex formation with ferredoxin as well as the enzymatic activities such as NADPH-diaphorase and NADPH-cytochrome c reductase activities. The complex formation of immobilized FNR with ferredoxin was investigated by measuring reflex spectra of the immobilized FNR with or without ferredoxin and by titration with ferredoxin. The experimental results obtained for the dissociation constant, pH profile and effect of salts were coincidental with those reported for the free enzyme system.
D004800:Enzymes, Immobilized / Q000378:metabolism*; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism*; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010944:Plants / Q000201:enzymology; D012685:Sepharose; D013053:Spectrophotometry
D004800:Enzymes, Immobilized; D012685:Sepharose; D005287:Ferredoxin-NADP Reductase; D009247:NADH, NADPH Oxidoreductases