An animal model of occupational immunologic asthma due to diphenylmethane diisocyanate, with multiple systemic immunologic responses.
Inhalation of MDI in the plastics industry may produce hypersensitivity pneumonitis or immediate-type asthma in workers. A suspension of MDI was given intrabronchially to dogs to determine whether any of the clinical and immunologic human responses could be duplicated, Results showed that dogs produce a systemic immune response to MDI-DSA. Serum MDI-125I-DSA binding was shown by the ammonium sulfate binding technique. Antibody activity of IgG, IgA, and IgM antibody classes was shown by a solid-phase enzyme-linked immunoassay. Precipitating antibodies were detected in he dog serum against MDI-DSA. Immediate-type skin reactivity to MDI-DSA consistent with a canine IgE response occurred. The skin reactivity appeared to correlate with an immediate-type airway response characterized by an increase in pulmonary resistance after intrabronchial MDI. There was transient peripheral blood lymphocyte reactivity to MDI-DSA. The results indicate that a large animal model of human immune response to MDI is available for various immunopathogenic studies.
D000403:Airway Resistance; D000542:Alveolitis, Extrinsic Allergic / Q000139:chemically induced* / Q000276:immunology; D000818:Animals; D000917:Antibody Formation; D001249:Asthma / Q000139:chemically induced* / Q000276:immunology; D001980:Bronchi / Q000187:drug effects; D003485:Cyanates / Q000009:adverse effects*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D017953:Isocyanates; D008214:Lymphocytes / Q000276:immunology; D009784:Occupational Diseases / Q000139:chemically induced* / Q000276:immunology; D012709:Serum Albumin / Q000276:immunology; D012711:Serum Albumin, Radio-Iodinated; D012882:Skin Tests; D001559:Benzhydryl Compounds
D003485:Cyanates; D007073:Immunoglobulin E; D017953:Isocyanates; D012709:Serum Albumin; D012711:Serum Albumin, Radio-Iodinated; C005969:4,4'-diphenylmethane diisocyanate; D001559:Benzhydryl Compounds