Determination of ophidine in human urine.
Ophidine (beta-alanyl-3-methylhistidine) was first detected in the urine of two patients and later in two members of the laboratory staff loaded with whale meat, by column chromatography, high-voltage paper electrophoresis and two-dimensional paper chromatography. The ophidine peak was detected between homocarnosine and dimethylarginine using a lithium buffer gradient in column chromatography. In paper chromatography the ophidine spot was detected at a position close to anserine and homocarnosine. The ophidine in the urine from the patients was of dietary origin since it was absent in the urine a few weeks later.
D000818:Animals; D000861:Anserine / Q000652:urine*; D002415:Cats; D002854:Chromatography, Paper; D004032:Diet; D004151:Dipeptides / Q000652:urine*; D004289:Dolphins; D004590:Electrophoresis, Paper; D006736:Horses; D006801:Humans; D008460:Meat; D009132:Muscles / Q000032:analysis; D011200:Poultry; D011817:Rabbits; D012104:Reptiles; D014907:Whales
D004151:Dipeptides; D000861:Anserine