title: [Lipid dependence of cytochrome P-450 activity in rat liver microsomes using lipid exchange proteins].
abstract: Modification of membrane lipid composition by lipid exchange proteins was used to study the lipid dependence of cytochrome P-450 activity in rat liver microsomes. The introduction of exogenous lysophosphatidylcholine into microsomal membranes decreased the capacity of cytochrome P-450 to demethylate dimethylaniline and dimethylaminoantipyrine and to hydroxylate aniline. A subsequent introduction of phosphatidylcholine resulted in an almost complete reactivation of the enzyme.
mesh_terms: D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D007700:Kinetics; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D008297:Male; D008563:Membrane Lipids / Q000502:physiology*; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D013379:Substrate Specificity
chemical_list: D008244:Lysophosphatidylcholines; D008563:Membrane Lipids; D003577:Cytochrome P-450 Enzyme System
vernacular_title: Izuchenie lipidnoĭ zavisimoti aktivnosti tsitokhroma P-450 mikrosom pechemi c pomoshch'iu lipidperenosiashchikh belkov.