Effects of RNA and ribonuclease on the binding of estrogen and glucocorticoid receptors from MCF-7 cells to DNA-cellulose.
Effects of RNA and ribonucleases on the binding of estrogen- and glucocorticoid-receptor complexes from MCF-7 cells to calf thymus DNA-cellulose have been studied under cell-free conditions. Exogenous ribonucleases can increase binding of estrogen- and glucocorticoid-receptor complexes to DNA-cellulose. Purified RNA derived from MCF-7 cells can inhibit estrogen- and glucocorticoid-receptor binding to DNA-cellulose. Under low salt conditions, RNA appears to be associated with estrogen-receptor complexes from MCF-7 cells. These observations taken together suggest that there is an interaction between steroid hormone-receptor complexes and RNA. The implications of this interaction are discussed.
D001943:Breast Neoplasms / Q000378:metabolism*; D002460:Cell Line; D002482:Cellulose / Q000031:analogs & derivatives; D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000031:analogs & derivatives; D003907:Dexamethasone / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006801:Humans; D007700:Kinetics; D012334:RNA, Neoplasm / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D011965:Receptors, Glucocorticoid / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism*
C033190:DNA-cellulose; D012334:RNA, Neoplasm; D011960:Receptors, Estrogen; D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D004958:Estradiol; D003907:Dexamethasone; D002482:Cellulose; D004247:DNA; D012260:Ribonucleases