Glucocorticoid receptor binding in bovine lens.
The present study demonstrates glucocorticoid receptor localization in the nuclei of cells of the anterior epithelium and bow region and its absence in all other regions of the lens. Both autoradiographic and biochemical studies show that triamcinolone acetonide is inactive whereas tetrahydrocortisol (previously considered to be an inactive metabolite of cortisol) is an active competitor of dexamethasone binding to the glucocorticoid receptor in the bovine lens. The consistency of these findings by both techniques supports the validity of this observation. In addition, it indicates that autoradiography can be used for studying glucocorticoid agonist/antagonist relationships in the human lens, where only small amounts of tissue are available.
D000818:Animals; D001345:Autoradiography; D002417:Cattle; D002467:Cell Nucleus / Q000378:metabolism; D003907:Dexamethasone / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism*; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D013760:Tetrahydrocortisol / Q000378:metabolism; D014221:Triamcinolone
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D014221:Triamcinolone; D013760:Tetrahydrocortisol; D003907:Dexamethasone