Uptake and calcium-dependent release of ethylenediamine (1,2-diaminoethane) by rat brain slices.
The uptake of [14C]ethylenediamine into slices of rat brain and its subsequent evoked release have been studied. An active uptake process was demonstrated by comparing uptake at 37 and 4 degrees C. This uptake showed a Km of 1.36 mM, was partly sodium-dependent and was reduced by nipecotic acid. Release could be readily evoked by 30 mM potassium, and by electrical stimulation, the release in both cases being calcium-dependent. In view of these findings and the reported interactions of ethylenediamine with gamma-aminobutyric acid-related mechanisms, it might be of interest to determine whether this simple diamine occurs endogenously in the mammalian brain.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000378:metabolism*; D005029:Ethylenediamines / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D009557:Nipecotic Acids / Q000494:pharmacology; D051381:Rats; D012964:Sodium / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D005029:Ethylenediamines; D009557:Nipecotic Acids; D005680:gamma-Aminobutyric Acid; C031234:ethylenediamine; D012964:Sodium; D002118:Calcium