Adenosine phosphate and glutathione levels in the regenerated corneal epithelium after abrasion and mild alkali burns.
After scraping away the corneal epithelium or after mild alkali burn, the regeneration of the cellular layers on the stromal surface was observed clinically. The average time of regeneration was 7 days after abrasion and 12 days after mild alkali burn. When the stromal surface was just covered by the newly regrown epithelium, the metabolite levels were determined. In the regenerated corneal epithelium after abrasion the ATP/ADP ratios were much higher than in the normal epithelium and much lower after the alkali burns. The GSH levels were diminished in both groups. The GSSG levels were elevated significantly only after the alkali burns resulting in a decreased redox ratio of the glutathione. The results seemed to be in accordance with morphological and enzymatical data of the regenerating corneal epithelium. Changes of the glutathione in the corneal endothelium were cautiously interpreted.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000468:Alkalies; D000818:Animals; D002057:Burns, Chemical / Q000378:metabolism; D003315:Cornea / Q000378:metabolism* / Q000502:physiology*; D004848:Epithelium / Q000378:metabolism; D005126:Eye Burns / Q000139:chemically induced / Q000378:metabolism; D005131:Eye Injuries / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D011817:Rabbits; D012038:Regeneration
D000227:Adenine Nucleotides; D000468:Alkalies; D005978:Glutathione