Salivary antibody response and priming stimulated by soluble or particulate antigens injected at a remote secretory site.
The salivary antibody response produced after remote secretory site (mammary gland) injection of soluble or particulate DNP antigens was examined in the rat. IgA and IgG salivary antibody and IgG serum antibody concentrations were determined by an ELISA technique. Two experiments were performed using DNP-BGG as the soluble antigen and either DNP-S. mutans or DNP-BGG-S. mutans as particulate antigens. Approximately equivalent IgA antibody responses were produced when soluble or particulate antigens were injected locally at a secretory site. However, a greater salivary IgA response was observed with the soluble antigen when the antigens were injected in the vicinity of a secretory site (mammary glands) remote from the salivary glands (experiment 1: 123 +/- 46 vs 2 +/- 1 ng IgA per millilitre of saliva, and experiment 2: 212 +/- 59 vs 45 +/- 21 ng IgA per millilitre of saliva). In both experiments, prior remote secretory site injection of the soluble antigen was able to prime for a significantly increased salivary IgA response (more than three-fold) after a local injection in the vicinity of the salivary gland. The particulate antigens did not lead to priming of the salivary antibody response. Prior remote secretory site injection of soluble antigen, but not of the particulate antigens, also increased salivary and serum IgG antibody levels above the levels observed with an injection at either site alone.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000008:administration & dosage; D000942:Antigens, Bacterial / Q000276:immunology*; D005260:Female; D007070:Immunoglobulin A / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007156:Immunologic Memory; D008321:Mammary Glands, Animal / Q000276:immunology*; D009578:Nitrobenzenes / Q000276:immunology; D011247:Pregnancy; D051381:Rats; D012463:Saliva / Q000276:immunology*; D012469:Salivary Glands / Q000276:immunology*; D012995:Solubility; D013295:Streptococcus mutans / Q000276:immunology
D000941:Antigens; D000942:Antigens, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D009578:Nitrobenzenes