Kinetic enzymatic determination of creatinine by a rapid semiautomated procedure.
The enzymatic method of creatinine determination, based on the transformation of creatinine to creatine by creatinine amido-hydrolase (EC 3.5.2.10) and subsequent evaluation of the creatinine, has been automated as a kinetic initial rate procedure. This was achieved by using a preincubation period under conditions that reduce the interference by accessory reactions to a very low and constant value, followed by a relatively high concentration of creatinine amido-hydrolase as a starter.
D003402:Creatine Kinase / Q000378:metabolism; D003404:Creatinine / Q000097:blood*; D006801:Humans; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D011933:Reagent Kits, Diagnostic; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D011933:Reagent Kits, Diagnostic; D003404:Creatinine; D007770:L-Lactate Dehydrogenase; D011770:Pyruvate Kinase; D003402:Creatine Kinase