Coulometric acid-base titration in nanoliter samples with glass and antimony electrodes.
A modification of the coulometric acid-base titration method is described for the measurement of titratable acid and ammonium in nanoliter samples. The main components consist of a pH-seeking device and a miniaturized antimony electrode system capable of delivering OH- ions at a known rate. The modifications include use of a cup-shaped pH-sensitive glass-membrane electrode for monitoring pH changes during titration and a new electronic titration circuit consisting of a high-impedance differential electrometer, a constant-current source, and an electronic integrating circuit. Details regarding the construction of the components of the titration system and practical aspects of its use are also provided.
D000136:Acid-Base Equilibrium; D000818:Animals; D000965:Antimony; D004566:Electrodes; D004594:Electrophysiology / Q000379:methods; D005898:Glass; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000502:physiology*; D007684:Kidney Tubules / Q000502:physiology
D000965:Antimony