Synthesis of a defective H-2 histocompatibility antigen by mutant mouse strain BALB/c-H-2dm2.
Mutant mouse strain BALB/c-H-2dm2 (dm2), which fails to express the H2Ld histocompatibility antigen associated with the wild type, BALB/c, synthesizes instead a smaller molecule that is structurally related to H-2Ld but does not carry detectable alloantigenic determinants. This new protein, p40, is a membrane glycoprotein found in dm2 cells but not in BALB/c. p40 was detected by electrophoresis of dm2 glycoprotein preparations and by immunoprecipitation with heterologous H-2-specific antibodies. The p40 molecule is found associated with intracellular membranes but was not detected at the cell surface. Peptide mapping studies suggest that dm2 carries an alteration in the H-2Ld structural gene, which prevents proper maturation of the protein product.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D006183:H-2 Antigens / Q000235:genetics* / Q000302:isolation & purification; D051379:Mice; D008817:Mice, Mutant Strains / Q000235:genetics* / Q000276:immunology; D013154:Spleen / Q000276:immunology
D006023:Glycoproteins; D006183:H-2 Antigens