Preparation of a biologically active rat prolactin devoid of antidiuretic activity.
Rat prolactin (NIH rPRL-B2) was purified using Sephadex chromatography and isoelectric focusing. SDS-gel electrophoresis of the original material showed a major band of 23K daltons, as well as several minor bands; the purified prolactin had a single 23K band. The original material contained 33 pg of immunoreactive vasopressin per 0.1 micrograms of material; vasopressin was not detectable in the purified material by either RIA or bioassay. The purified preparation had complete biological activity in a mammary gland bioassay and a receptor binding assay.
D000818:Animals; D002850:Chromatography, Gel; D008970:Molecular Weight; D011388:Prolactin / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D014667:Vasopressins / Q000032:analysis
D014667:Vasopressins; D011388:Prolactin