Phospholipid methylation and noradrenaline exchanges in a synaptosomal preparation from the rat brain.
When synaptosomes from rat brain were incubated with S-adenosylmethionine (10(-3) M), noradrenaline uptake and KCl-stimulated release were decreased. These effects were dependent on MgCl2, temperature, and incubation time. We have investigated the enzymatic methylation of phosphatidylethanolamine to give phosphatidylcholine.
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism*; D050918:Phosphatidylethanolamine N-Methyltransferase; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012436:S-Adenosylmethionine / Q000378:metabolism; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D015016:Yohimbine / Q000494:pharmacology
D010743:Phospholipids; D015016:Yohimbine; D012436:S-Adenosylmethionine; D008780:Methyltransferases; D050918:Phosphatidylethanolamine N-Methyltransferase; D009638:Norepinephrine