Kinetic characteristics of bicarbonate-chloride exchange across the neonatal human red cell membrane.
The kinetics of HCO-3/Cl- exchange across red cell membrane of newborn infants was studied using a stopped-flow rapid reaction apparatus with a glass pH electrode attached. The measured apparent permeability P is (1.35 +/- 0.08 (S.E.)). 10(-4) cm/s (n = 30) for newborns, compared with (3.1 +/- 0.4) . 10(-4) cm/s (n = 15) for adults. These correspond to half-times of 0.2 s for newborns and 0.1 s for adults indicating that neonatal red cells exchange Cl- for HCO-3 only half as fast as do adult cells. The temperature dependence of the exchange rate was studied from 2 to 42 degrees C. From the Arrhenius plot the activation energy of the exchange process in neonatal red cells changes from 22.9 kcal/mol (low temperature) to 4.8 kcal/mol (physiological temperature) at a transition temperature of 17 degrees C. These values are lower than the corresponding values for adult red cells, 34.7 and 10.2 kcal/mol. HCO-3/Cl- exchanges in both adult and neonatal red cells are inhibited by phlorizin. Inhibition constants Ki are 0.8 mM and 2.5 mM for adults and newborns, respectively. The differences in the value of the HCO-3/Cl- exchange rate constant and the activation energy of the exchange process between neonatal and adult red cells indicate that there is a modification of HCO-3/Cl- transport system in the neonatal red cell membranes.
D000328:Adult; D000375:Aging; D001639:Bicarbonates / Q000097:blood*; D001693:Biological Transport, Active; D002712:Chlorides / Q000097:blood*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D007700:Kinetics; D008875:Middle Aged; D013816:Thermodynamics
D001639:Bicarbonates; D002712:Chlorides