Inhibitors of protein glycosylation inhibit the differentiation of Friend erythroleukemia cells.
Tunicamycin, 2-deoxy-D-glucose and 2-deoxy-2-fluoro-D-glucose inhibit dimethyl sulfoxide-induced differentiation of Friend cells. This inhibition, characterized by inhibition of hemoglobin synthesis, is accompanied by a specific inhibition of protein glycosylation. The results of cloning experiments indicate that this inhibition specifically affects cells in the period preceding their commitment. These results suggest that glycoprotein synthesis is a requirement for Friend erythroleukemia cells in order to initiate the expression of the terminal differentiation program.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003847:Deoxyglucose / Q000031:analogs & derivatives / Q000494:pharmacology; D019788:Fluorodeoxyglucose F18; D006023:Glycoproteins / Q000096:biosynthesis*; D007942:Leukemia, Experimental / Q000503:physiopathology*; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism*; D014415:Tunicamycin / Q000494:pharmacology
D006023:Glycoproteins; D009363:Neoplasm Proteins; D019788:Fluorodeoxyglucose F18; D014415:Tunicamycin; D003847:Deoxyglucose