Measurement of fibrinogen in dog liver cell fractions by electroimmunoassay and radioimmunoassay.
Dog liver was fractionated and the amount of fibrinogen, in the various cell fractions (organelles) involved in the secretory process, was determined both by the Laurell electroimmunoassay and by radioimmunoassay. An unequal distribution of fibrinogen was noted for each cell fraction after an appropriate correction was made. This involved the estimation of the level of contamination of each cell fraction by soluble (blood) fibrinogen. It was observed that significant amounts of added 125I-labeled fibrinogen could be found in each organelle following cell fractionation. This was especially true for the rough endoplasmic reticulum which contained as much as 31% of the added tracer. Both the smooth endoplasmic reticulum and the Golgi fractions contained much lower amounts of added tracer. Using this correction factor, it was found that the rough endoplasmic reticulum contains about 0.1-0.2 micrograms fibrinogen per mg organelle protein. On the other hand, the smooth endoplasmic reticulum and Golgi fractions contained much more fibrinogen. The fibrinogen concentration ranges found for the latter two organelles were 1.9-5.0 and 1.5-5.7 micrograms fibrinogen per mg organelle protein, respectively.
D000818:Animals; D003594:Cytoplasmic Granules / Q000032:analysis; D004285:Dogs; D004721:Endoplasmic Reticulum / Q000032:analysis; D005340:Fibrinogen / Q000032:analysis*; D006056:Golgi Apparatus / Q000032:analysis; D007122:Immunoelectrophoresis; D008099:Liver / Q000032:analysis*; D008854:Microscopy, Electron; D011863:Radioimmunoassay; D014018:Tissue Distribution
D005340:Fibrinogen