Activity of nitro-polynuclear aromatic hydrocarbons in the sister chromatid exchange assay with and without metabolic activation.
Nitro-polynuclear aromatic hydrocarbons are found in diesel particulates. These compounds are potent mutagens in the Ames test. To determine whether nitro-polynuclear aromatic hydrocarbons are active in a mammalian cell assay, 1-nitropyrene, 1,8-dinitropyrene, 2-nitrofluorene, and 4-nitrobiphenyl were incubated with cultures of Chinese hamster ovary cells. The frequency of sister chromatid exchange (SCE) was measured in the presence and absence of rat liver S-9 mix. The addition of S-9 mix resulted in a large increase in the SCEs induced by all four compounds.
D000818:Animals; D001711:Biotransformation; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003434:Crossing Over, Genetic / Q000187:drug effects*; D005260:Female; D009153:Mutagens; D009574:Nitro Compounds / Q000633:toxicity; D011083:Polycyclic Compounds / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013347:Subcellular Fractions / Q000187:drug effects
D009153:Mutagens; D009574:Nitro Compounds; D011083:Polycyclic Compounds