[Preliminary results on the metabolism of 1,3-cyclohexadiene in isolates rat hepatocytes].
Hepatocytes of adult male rats were isolated by hepatic perfusion with a mixture of collagenase and hyaluronidase. This procedure gave a high yield of viable cells, as determined by trypan blue exclusion. The addition of 1,3-cyclohexadiene to a suspension of isolated liver cells gave rise to a type-I spectral change indicative of the formation of the cytochrome P-450-substrate complex. 1,3-cyclohexadiene was then incubated with hepatocytes for different times, to determine its biotransformation. Cyclohexadiene-1,2-diol resulted to be the main metabolite. It is proposed that the metabolic pathway of 1,3-cyclohexadiene involves the intermediate formation of an epoxide which is rapidly transformed to the correspondent diol.
D000818:Animals; D002469:Cell Separation; D003510:Cyclohexanes / Q000378:metabolism*; D053138:Cyclohexenes; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D003510:Cyclohexanes; D053138:Cyclohexenes; D003577:Cytochrome P-450 Enzyme System; C034005:1,3-cyclohexadiene
Risultati preliminari sul metabolismo dell'1,3-cicloesadiene con epatociti isolati di ratto.