title: Satellite versus total DNA replication in relation to endopolyploidy of decidual cells in the mouse.
abstract: In rodents, decidualization produces large endopolyploid cells. Amongst the various endocycles which have been demonstrated in animals and plants, different modes of DNA replication have been characterized: either total reproduction of all DNA types, or else, underreplication or amplified synthesis affecting specific parts of the genome. A double labelling method was used to determine to which of these categories the case of decidual cells belongs. A mixture of purified DNA from hormonally-stimulated control endometrium labelled by 3H-thymidine and from decidua labelled by 14C-thymidine was ultra-centrifuged to equilibrium in a Cs2 SO4-Ag gradient. Optical density at 260 nm and 14C/3H ratio were evaluated in serial fractions along the gradient. Since the 14C/3H ratio did not significantly vary along the gradient, it may be concluded that in the case of decidual cells, endopolyploidy corresponds to uniform replication of all nuclear DNA.
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D004261:DNA Replication; D004276:DNA, Satellite / Q000096:biosynthesis*; D003656:Decidua / Q000378:metabolism*; D005260:Female; D051379:Mice; D011123:Polyploidy; D011247:Pregnancy
chemical_list: D004276:DNA, Satellite