Dimethyl sulfoxide as a cholesterol-lowering agent in cultured fibroblasts exposed to low density lipoproteins.
Confluent cultures of human skin fibroblasts were exposed to medium containing high levels of low density lipoproteins (LDL-cholesterol equivalent to 400 micrograms per ml) and 0 or 2% dimethyl sulfoxide (DMSO). The uptake and accumulation of cellular cholesterol from LDL were reduced significantly (30%) in the DMSO-treated cells as compared to the controls. The reduction in cellular sterol was due almost exclusively to a significant decrease (50%) in cholesterol ester accumulation. Incubation of cells with 125I-labelled LDL showed clearly that DMSO did not act by increasing the secretion of cholesterol from the cell, but rather by significantly decreasing the binding, internalization and degradation of exogenous LDL. De novo synthesis of cholesterol from [14C]acetate was measured and found to correlate inversely with cellular sterol levels in either control or DMSO-treated cells.
D000085:Acetates / Q000378:metabolism; D000924:Anticholesteremic Agents; D002478:Cells, Cultured; D002784:Cholesterol / Q000096:biosynthesis / Q000378:metabolism*; D008078:Cholesterol, LDL; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D012867:Skin / Q000378:metabolism*
D000085:Acetates; D000924:Anticholesteremic Agents; D008078:Cholesterol, LDL; D008077:Lipoproteins, LDL; D002784:Cholesterol; D004121:Dimethyl Sulfoxide