Simplified fluorometry of total metanephrines in urine.
We describe a simplified fluorometric method for quantitatively determining metanephrines in urine. The method is based on the elution of metanephrines from an ion-exchange column followed by production of a fluorescent derivative that has excitation and emission maxima of 405 nm and 505 nm, respectively. Metanephrine concentrations as great as 7.0 mg/L were within the linear range of the assay. Analytical recovery of metanephrines was 82-87%. Cross contamination by catecholamines was insignificant. The upper range of normal was 520 micrograms/24 h. Within-run and between-run CV's were 5% and about 9%, respectively. Twenty-four-hour urine specimens from five patients with pheochromocytoma showed above-normal amounts of the metanephrines by this method.
D000310:Adrenal Gland Neoplasms / Q000652:urine*; D002395:Catecholamines / Q000652:urine; D002852:Chromatography, Ion Exchange; D004837:Epinephrine / Q000031:analogs & derivatives*; D005470:Fluorometry; D006801:Humans; D008676:Metanephrine / Q000652:urine*; D010673:Pheochromocytoma / Q000652:urine*; D012016:Reference Values
D002395:Catecholamines; D008676:Metanephrine; D004837:Epinephrine