Quantitation of beta-lipoprotein in cord serum by rate nephelometric immunoassay: a potential screening test for familial hypercholesterolemia.
We measured beta-lipoprotein in 232 cord sera by rate nephelometry and compared the results with those obtained by electroimmunoassay. The mean (mean +/- two standard deviations) was 544 (293-1009) mg/L by rate nephelometry and 503 (265-955) mg/L by electroimmunoassay. The between-assay correlation coefficient was 0.891. Between-run coefficients of variation were 3.5-4.4% for nephelometry and 11-14% for electroimmunoassay. Nephelometry was faster, more precise, and less labor intensive than electroimmunoassay. For these reasons, rate nephelometry appears to be a more suitable assay system for mass screening, should detection of familial hypercholesterolemia prove feasible and desirable.
D001724:Birth Weight; D005260:Female; D005312:Fetal Blood / Q000032:analysis*; D005865:Gestational Age; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000097:blood*; D007118:Immunoassay; D007231:Infant, Newborn; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D008403:Mass Screening; D009391:Nephelometry and Turbidimetry; D012016:Reference Values
D008077:Lipoproteins, LDL