title: The temperature dependence of recovery of metabolic function following hypothermic potassium cardioplegic arrest.
abstract: A study was performed to define the influence of reperfusate temperature on the recovery of high-energy phosphate compounds following hypothermic ischemic cardioplegic arrest. Two groups, each consisting of six pigs, were subjected to 1 hour of hypothermic potassium cardioplegic arrest (myocardial temperature 10 degrees to 15 degrees C). In one group, the arrest period was followed by a 30 minute period of normothermic reperfusion (37 degrees C) followed by a second hour of hypothermic cardioplegic arrest and a second 30 minute normothermic reperfusion period. The second group of pigs was reperfused after the first hour of arrest for 30 minutes with a cold blood (10 degrees to 14 degrees C) followed by an additional hour of hypothermic cardioplegic arrest and a second 30 minute hypothermic reperfusion period. Myocardial biopsy specimens for adenosine triphosphate (ATP) and creatine phosphate (CP) were obtained at the beginning of perfusion (control studies), at the end of each hour of the arrest interval, and at the end of each 30 minutes of reperfusion. The ATP and CP levels were significantly higher after normothermic reperfusion than after hypothermic reperfusion. After normothermic reperfusion the CP level exceeded control; following hypothermic reperfusion CP remained less than 70% of the control level. On the basis of these results, it is proposed that hypothermic reperfusion does not permit the same level of high-energy phosphorylation that is provided with normothermic reperfusion and may therefore adversely affect hemodynamic recovery of the heart after cardioplegic arrest.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D006324:Heart Arrest, Induced; D007036:Hypothermia, Induced; D009206:Myocardium / Q000378:metabolism*; D010477:Perfusion / Q000379:methods; D010725:Phosphocreatine / Q000378:metabolism; D013552:Swine; D013696:Temperature
chemical_list: D010725:Phosphocreatine; D000255:Adenosine Triphosphate