Proteodermatan sulfate isolated from pig skin.
The major proteoglycan of pig skin, a proteodermatan sulfate, has been isolated under mild conditions in the presence of protease inhibitors. After purification by ion exchange and gel chromatography, it has a Mr of 70 x 10(3) and it does not form aggregates with hyaluronic acid. It contains about 60% protein, one chain of dermatan sulfate, and several oligosaccharide chains. The dermatan sulfate is high in iduronic acid (85% of the uronic acid) and there are no chains of chondroitin sulfate present. The oligosaccharides contain glucosamine, sialic acid, and neutral sugars.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002807:Chondroitin / Q000031:analogs & derivatives*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003871:Dermatan Sulfate / Q000031:analogs & derivatives* / Q000302:isolation & purification; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis; D011509:Proteoglycans / Q000302:isolation & purification*; D012867:Skin / Q000032:analysis*; D013552:Swine
D000596:Amino Acids; D002241:Carbohydrates; D009844:Oligosaccharides; D011509:Proteoglycans; C028774:proteodermatan sulfate; D003871:Dermatan Sulfate; D002807:Chondroitin