Determination of cyclobenzaprine in tablets by high-performance liquid chromatography.
A convenient high-performance liquid chromatographic method for the quantitative determination of cyclobenzaprine hydrochloride in tablets is described. Samples were dissolved in 0.05 N sulfuric acid and diluted with methanol. The cyclobenzaprine hydrochloride was chromatographed using an octylsilane column and the eluent acetonitrile-0.6% dibasic potassium phosphate aqueous buffer (pH 3.0) (75:25) at a flow rate of 1.5 ml/min. Naphazoline hydrochloride was used as an internal standard. The UV detector response at 254 nm was linear for cyclobenzaprine hydrochloride in the 0.005-0.03 mg/ml range under conditions of the analysis.
D000639:Amitriptyline / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006863:Hydrogen-Ion Concentration; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets
D013607:Tablets; D000639:Amitriptyline; C004704:cyclobenzaprine