Effect of alcohol and carbon monoxide on the pH-activity relationship of erythrocytic delta-aminolevulinate dehydratase (ALA-D).
The effect of blood alcohol on the pH-activity profile of ALA-D in humans and rabbits has been measured. The shape of the pH-activity profile remains unchanged, although enzyme activities are significantly affected (up to 40%). In order for the blood lead assay using ALA-D to remain independent of the presence of alcohol, an alternative methodology, such as a ratio of activities at two pH values, and not a measurement at a single pH value should be used. The effect of carbon monoxide on the pH-activity profile of ALA-D, on the other hand, is minimal.
D000818:Animals; D002249:Carbon Monoxide Poisoning / Q000097:blood / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D000431:Ethanol / Q000633:toxicity*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007854:Lead / Q000097:blood; D000623:Porphobilinogen Synthase / Q000037:antagonists & inhibitors* / Q000097:blood; D011817:Rabbits
D007854:Lead; D000431:Ethanol; D000623:Porphobilinogen Synthase