Isolation of granulocytes and labelling with indium 111-oxine sulphate.
The isolation of granulocytes from whole blood and labelling with Indium 111-oxine sulphate are described in detail. To isolate the cells a two-step method was used: (1) removal of the red blood cells by methyl cellulose-Ronpacon and (2) separation of the leucocyte-rich plasma with a double gradient (1077, 1097) technique. 111In-oxine sulphate was prepared by adding 111In-chloride to a buffered solution of oxine sulphate. The labelling of the granulocytes with 111In-oxine sulphate was done by incubation at room temperature for 5 min.
D002469:Cell Separation; D006098:Granulocytes; D006801:Humans; D007204:Indium; D007553:Isotope Labeling; D009942:Organometallic Compounds; D015125:Oxyquinoline / Q000031:analogs & derivatives*; D011868:Radioisotopes
D009942:Organometallic Compounds; D011868:Radioisotopes; D007204:Indium; C032950:indium oxine; D015125:Oxyquinoline