Thyroxine-stimulated synthesis of microvillus membrane glycoproteins during culture of chick embryonic duodenum.
The effect of thyroxine on biosynthesis of microvillus membrane glycoproteins has been investigated in organ culture of 18-day-old chick embryonic duodenum. Explants incorporate [3H]leucine and [3H]glucosamine continuously, and overall incorporation is enhanced by 10 nM thyroxine during 48 h of labeling; this increase in radioactivity is associated with vesicles released from the microvilli. Light microscope autoradiography, pulse labeling of brush border fragments, and pulse chase experiments reveal that [3H]glucosamine is incorporated into brush border at an increasing rate during culture, and that newly synthesized glycoproteins are discharged into the medium along with brush border enzymes (alkaline phosphatase and maltase). These results suggest that thyroxine stimulates biosynthesis of microvillus membrane glycoproteins, in addition to stimulating vesiculation of the membrane. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of 3H-labeled vesicles and brush border fragments show that [3H]leucine and [3H]glucosamine are incorporated into proteins of high molecular weight. Two protein bands are identified as alkaline phosphatase and maltase. Thyroxine stimulates glycosylation of these enzymes, but does not change protein patterns. Radioactivity assay of alkaline phosphatase- and maltase-active gel slices suggests that thyroxine stimulation of these enzyme activities during culture is not correlated with de novo synthesis of these proteins.
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects*; D002642:Chick Embryo; D004386:Duodenum / Q000166:cytology / Q000187:drug effects*; D006023:Glycoproteins / Q000096:biosynthesis*; D007413:Intestinal Mucosa / Q000648:ultrastructure; D008565:Membrane Proteins / Q000096:biosynthesis*; D008871:Microvilli / Q000187:drug effects*; D009924:Organ Culture Techniques; D013974:Thyroxine / Q000494:pharmacology*
D006023:Glycoproteins; D008565:Membrane Proteins; D013974:Thyroxine