Determination of plasma levels of spirorenone, a new aldosterone antagonist, and one of its metabolites by high-performance liquid chromatography.
A method for the determination of plasma concentrations of spirorenone, a new aldosterone antagonist, and one of its metabolites, chromatographically characterized as 1,2-dihydro-spirorenone, is described. The assay utilizes high-performance liquid chromatography with UV detection. Reproducible results can be obtained with standard deviations of about 5% and the limit of detection is less than 5 ng/ml. Plasma levels of drug and metabolite have been measured after oral doses of 10 and 40 mg, respectively, administered to two male volunteers.
D000328:Adult; D000730:Androstadienes / Q000097:blood*; D000736:Androstenes / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D006801:Humans; D008297:Male; D000451:Mineralocorticoid Receptor Antagonists / Q000097:blood* / Q000494:pharmacology; D012016:Reference Values; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D000730:Androstadienes; D000736:Androstenes; D000451:Mineralocorticoid Receptor Antagonists; C035143:spirorenone; C035144:drospirenone