Purification of human C3b inactivator by monoclonal-antibody affinity chromatography.
Monoclonal antibody has been obtained to the human complement control protein C3b inactivator after immunization of mice with the enzyme prepared by conventional methods. Antibody from ascitic fluid was purified and coupled to Sepharose-CL-4B to give a specific affinity column, which was used to isolate C3b inactivator from human serum in 70% yield. The product was characterized by size, chain structure, amino acid analysis and proteolytic activity.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity / Q000379:methods; D003180:Complement C3b Inactivator Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D011863:Radioimmunoassay
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D003180:Complement C3b Inactivator Proteins