Studies on colony specificity in the compound ascidian, Botryllus primigenus Oka. II. In vivo bioassay for analyzing the mechanism of "nonfusion" reaction.
Fusion experiments with three colonies (AC-BC-BD) by Tanaka and Watanabe indicated that the mixture of blood from two nonfusible colonies induced a "nonfusion" reaction (NFR)-like responses. Thus, artificial mixture of the blood from two nonfusible colonies is expected to result in NFR-like reactions. The present study deals with an in vivo bioassay using syngeneic and allogeneic blood injections. Syngeneic injection of whole blood induced no response, while allogeneic injection of whole blood induced inflammatory responses indicating recognition of allogeneic tissue. Syngeneic injection of blood plasma or blood cells induced no response. On the other hand, allogeneic injection of blood cells resulted in a response similar to that of whole blood injections. Allogeneic blood plasma induced only weak responses. It is hypothesized that NFR is initiated by the mutual interaction between blood cells and blood humoral factor(s).
D000818:Animals; D006434:Hemocytes / Q000276:immunology; D006458:Hemolymph / Q000276:immunology; D007249:Inflammation / Q000209:etiology; D007267:Injections; D012965:Sodium Chloride / Q000494:pharmacology; D013045:Species Specificity; D014561:Urochordata / Q000166:cytology / Q000276:immunology*
D012965:Sodium Chloride