Sequences of ribosome binding sites from the large size class of reovirus mRNA.
Ribosome-protected fragments from two of the large-sized reovirus mRNAs were recovered from sparsomycin-blocked 80S initiation complexes. The sequence of each protected oligonucleotide was determined. The ribosome binding site of each message includes the m7G cap and a centrally positioned AUG codon. An adenine residue occurs three nucleotides upstream from the initiator codon, thus conforming to the pattern [AG]NNAUG observed for nearly all eucaryotic initiation sites.
D001483:Base Sequence; D001665:Binding Sites; D003062:Codon; D012089:Mammalian orthoreovirus 3 / Q000235:genetics*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis; D010442:Peptide Chain Initiation, Translational; D012315:RNA Caps / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012087:Reoviridae / Q000235:genetics*; D012270:Ribosomes / Q000378:metabolism
D003062:Codon; D009841:Oligonucleotides; D012315:RNA Caps; D012333:RNA, Messenger; D012367:RNA, Viral