Content of glucocorticoid type III receptors in tissue cytosol.
The Scatchard analysis was used to determine the association and dissociation constants and the number of binding sites for corticosterone over a glucocorticoid type III receptor in high-speed cytosol from liver, kidneys, heart and lungs of intact male rats after coal adsorption of endogenous glucocorticoids. The association constants for the liver, kidney, heart and lung cytosols were 0.48 X 10(10) M-1, 0.34 X 10(10) M-1, 0.175 X 10(10) M-1 and 0.96 X 10(10) M-1, respectively, and the binding site numbers 0.57 X 10(-3), 2.4 X 10(-10), 4.5 X 10(-13) and 6.4 X 10(-13) mole/mg protein, respectively. The authors have suggested a hypothesis that glococorticoid type III receptors take part in regulating the intracellular transport of glucocorticoids.
D000818:Animals; D003345:Corticosterone / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; C024357:corticosterone receptor; D003345:Corticosterone