Antigenic structure of sperm whale myoglobin. I. Partition of specificities between antibodies reactive with peptides and native protein.
We have resolved anti-sperm whale Mb antibodies into two distinct populations: One population is reactive only with the native molecule, and the second is reaction with both Mb and peptide fragments. CNBr cleavage of sperm whale Mb yields three peptides: peptide I (1-55), peptide II (56-131), and peptide III (132-153). Immunoadsorbent columns made with these three peptides were used to fractionate antibody to sperm whale Mb. These columns cumulatively bind 60 to 70% of the total antibody present in the four sera studied (the amount of antibody bound by each peptide was proportional to its size). The remaining 30 to 40% of antibody could only bind specifically to an immunoadsorbent column of native Mb. This result indicates that not all antigenic determinants on the native Mb molecule are represented on peptide fragments.
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000145:classification* / Q000302:isolation & purification; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000941:Antigens; D001059:Apoproteins; D001666:Binding Sites, Antibody; D002591:Cetacea / Q000276:immunology*; D055598:Chemical Phenomena; D002621:Chemistry; D009211:Myoglobin / Q000276:immunology* / Q000302:isolation & purification; D010455:Peptides / Q000276:immunology; D011487:Protein Conformation; D011817:Rabbits; D014907:Whales / Q000276:immunology*
D000906:Antibodies; D000941:Antigens; D001059:Apoproteins; D009211:Myoglobin; D010455:Peptides; C010857:apomyoglobin