Effect of hydralazine on hepatic drug metabolizing enzymes in rats.
The effects of hydralazine on hepatic drug metabolizing enzymes were studied in rats. Hydralazine inhibited aminopyrine N-demethylase and aniline hydroxylase in vitro. Kinetic studies indicated that the inhibitory natures of hydralazine were recognized as almost mixed types and a change in difference spectrum demonstrated that hydralazine could bind to cytochrome P-450 as a characteristic type II. A single administration of hydralazine inhibited the drug metabolism, but neither reduction nor induction of the mixed function oxidase system were observed after the hydralazine treatment for 7 days, except the reduction of cytochrome b5 content. In addition, acidophilic degeneration and the formation of acidophilic bodies in liver cells by hydralazine were observed.
D000818:Animals; D006830:Hydralazine / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D008297:Male; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D006830:Hydralazine; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases