Laboratory confirmation of phenylketonuria. Combined paper chromatography and "inhibition assay".
It is absolutely necessary to confirm any positive screening result for phenylketonuria by two or more independent procedures carried out on two or more different blood specimens before initiation of diet treatment. Details for a combined paper chromatography and "inhibition assay" of cut paper strips for positive identification of blood phenylalanine are given. This procedure correlates the partition between two solvents, the reaction to ninhydrin and the reaction in "inhibition assay".
D002854:Chromatography, Paper / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn; D010649:Phenylalanine / Q000097:blood; D010661:Phenylketonurias / Q000097:blood / Q000175:diagnosis*
D010649:Phenylalanine