Studies on the mutagenic potential of the pesticide chlordimeforme and its principal metabolites in the mouse heritable translocation assay.
Chlordimeforme and its 2 principal metabolites, N-formyl-4-chloro-o-toluidine and 4-chloro-o-toluidine, were studied for induction of heritable translocations. Maximal tolerated doses of the compounds were given daily by gavage for 7 consecutive weeks. After mating with untreated females, about 1000 F1 male offspring per group--including vehicle control and positive control (Tretamine, TEM)--were tested for their reproductive performance by use of a sequential decision procedure on litter sizes to select males with translocation heterozygosity. Partially sterile, sterile and non-classifiable F1 males were examined cytogenetically by scoring meiotic chromosomes for translocation multivalents or analysing mitotic divisions for marker chromosomes. The 3 compounds tested at dose levels showing toxic effects did not induce translocation heterozygosity. Tretamine, the positive control, gave the expected mutagenic response.
D000578:Amidines / Q000494:pharmacology*; D000818:Animals; D002742:Chlorphenamidine / Q000031:analogs & derivatives / Q000494:pharmacology*; D003433:Crosses, Genetic; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D009153:Mutagens; D014052:Toluidines / Q000494:pharmacology*; D014178:Translocation, Genetic
D000578:Amidines; D009153:Mutagens; D014052:Toluidines; C556393:p-chloro-o-toluidine hydrochloride; C006996:4'-chloro-2-formotoluidide; D002742:Chlorphenamidine