[Comparison of commercially available radioimmunoassays for the determination of bile acids in serum (author's transl)].
Three commercially available radioimmunoassays for the determination of bile acids in serum were evaluated with respect to specificity and precision. The SLCG-radioimmunoassay (Abbott) measures chiefly cholic acid conjugates, and the CBA-radioimmunoassay (Becton-Dickinson) measures all conjugated bile acids, with an over-response to taurine metabolites. With respect to cross reactions, the performances of the CG-and the CBA-radioimmunoassays differed significantly from those stated by the manufacturers, the former showing a 32% response to taurocholic acid, the latter responding only 118% to taurochenodeoxycholic acid. At physiological concentrations of albumin + globulin, the recovery of defined cholanic acids was 85-101%. Good reproducibility was shown by the CG-radioimmunoassay in the range 0.5-10.0 mumol/l, by the CBA-radioimmunoassay in the range 1.0-25.0 mumol/l, and by the SLCG-radioimmunoassay in the range 0.5-3.0 mumol/l. There were no important differences in the inter-and intra-assay precision of the three methods.
D000918:Antibody Specificity; D001647:Bile Acids and Salts / Q000097:blood*; D003429:Cross Reactions; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods
D001647:Bile Acids and Salts
Vergleich kommerziell erhältlicher Radioimmunoassays zur Bestimmung von Gallensäuren im Serum.