The measurement of serum cortisol by a solid-phase chemiluminescence immunoassay.
A solid-phase chemiluminescent immunoassay (LIA) for the determination of cortisol in serum specimens has been developed. The separation of the cortisol onto the solid-phase distinguishes this particular assay from other chemiluminescence immunoassays. The method can be used without prior extraction. Cortisol molecules were made chemiluminescent by conjugation to aminobutylethyl-isoluminol. The detection limit of the label in the chemiluminescent reaction was 0.1 pmol. The LIA was compared with a RIA for cortisol in 36 serum specimens and the correlation between the assays was good (r = 0.97). The time course of the chemiluminescent reaction is fast and a 20's integration time gave reproducible results. The peak light intensities were not reliable.
D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D007118:Immunoassay / Q000379:methods; D008163:Luminescent Measurements; D008832:Microchemistry / Q000379:methods; D013997:Time Factors
D006854:Hydrocortisone