Simultaneous determination of vitamins A and D in a dosage forms by high pressure liquid chromatography.
Vitamins A and D were determined simultaneously in oily solutions, ointments, and elixirs, but only vitamin A could be determined in capsules. Samples were saponified with KOH in isopropanol-water, using hydroquinone as antioxidant, and extracted with ether-petroleum ether (1 + 1). After evaporation of solvent, residues were dissolved in isopropanol. Vitamins in these solutions were determined by reverse phase high pressure liquid chromatography, using methanol-water as mobile phase and detection at 254 nm. The reproducibility, using external standards, was 1.6-2.5% and 1.2-3.8% for vitamins A and D, respectively.
D002214:Capsules / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D009824:Ointments / Q000032:analysis; D012996:Solutions / Q000032:analysis; D014801:Vitamin A / Q000032:analysis*; D014807:Vitamin D / Q000032:analysis*
D002214:Capsules; D009824:Ointments; D012996:Solutions; D014801:Vitamin A; D014807:Vitamin D