Potential peptic activity of pepsinogen of human gastroduodenal mucosa determined by fluorescent microassay method using succinyl albumin.
Pepsinogen level, expressed as the potential peptic activity of pepsinogen was determined by a fluorescent microassay using succinyl albumin as substrate, in biopsy specimens from the gastroduodenal mucosa of 95 subjects. The following results were obtained: (1) pepsinogen level in the gastric mucosa becomes progressively higher from pylorus to corpus (p less than 0.001); (2) pepsinogen level in the gastroduodenal mucosa of duodenal ulcer was significantly higher than that of normal mucosa or gastric ulcer (p less than 0.001); (3) the difference in pepsinogen level between the gastric mucosa with and without intestinal metaplasia was statistically significant (p less than 0.01); (4) the correlation between the histology of gastric glands and pepsinogen level was fundic gland greater than intermediate gland greater than pyloric gland (p less than 0.001).
D000328:Adult; D000375:Aging; D000418:Albumins; D004381:Duodenal Ulcer / Q000378:metabolism; D004386:Duodenum / Q000378:metabolism*; D005450:Fluorescamine; D005753:Gastric Mucosa / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000473:pathology; D008679:Metaplasia / Q000378:metabolism; D008832:Microchemistry; D008875:Middle Aged; D010435:Pepsinogens / Q000378:metabolism*; D012709:Serum Albumin; D013276:Stomach Ulcer / Q000378:metabolism; D014018:Tissue Distribution
D000418:Albumins; D010435:Pepsinogens; D012709:Serum Albumin; C034877:succinylalbumin; D005450:Fluorescamine