A rational approach to the fixation of peptidergic nerve cell bodies in the gut using parabenzoquinone.
Study of the possible chemical mechanisms by which parabenzoquinone acts as a fixative in the localisation of neuronal peptides by immunohistochemistry suggested several possible improvements to standard techniques. In particular, by using favourable conditions for regenerative oxidation reactions, i.e. fixing at 37 degrees C in well oxygenated, slightly alkaline buffer, it was possible to improve the localisation of substance P-, vasoactive intestinal polypeptide-, enkephalin- and somatostatin-like immunoreactive peptides such that nerve cell bodies were routinely visualised without the use of drugs to block axonal transport. Application of the improved fixation method is likely to facilitate study of the organisation of peptidergic systems.
D000818:Animals; D001369:Axons / Q000378:metabolism; D016227:Benzoquinones; D004064:Digestive System / Q000378:metabolism; D005404:Fixatives; D006168:Guinea Pigs; D006651:Histocytochemistry; D007120:Immunochemistry; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011809:Quinones; D051381:Rats
D016227:Benzoquinones; D005404:Fixatives; D010455:Peptides; D011809:Quinones; C004532:quinone