Characteristics and specificity of phenelzine and benserazide as inhibitors of benzylamine oxidase and monoamine oxidase.
The selectivity of benserazide and phenelzine toward inhibition of benzylamine oxidase (BzAO) and monoamine oxidases (MAO-A and MAO-B) was studied in homogenates of rat skull and lung. In addition, the kinetic interaction and reversibility of BzAO inhibition were assessed. Both drugs inhibited BzAO but only phenelzine inhibited MAO, whether tested in vitro or in vivo. Neither compound acted as an irreversible inhibitor of BzAO. Benserazide was found to be a noncompetitive inhibitor. Phenelzine acted as a substrate for BzAO followed by product-induced noncompetitive inhibition which was labile at 37 degrees but not at 4 degrees. A reversible component in phenelzine-induced inhibition of MAO-A and -B is also suggested from in vivo studies.
D000818:Animals; D001545:Benserazide / Q000494:pharmacology*; D001595:Benzylamine Oxidase / Q000037:antagonists & inhibitors*; D006834:Hydrazines / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D008995:Monoamine Oxidase; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology*; D010624:Phenelzine / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D006834:Hydrazines; D008996:Monoamine Oxidase Inhibitors; D001545:Benserazide; D001595:Benzylamine Oxidase; D008995:Monoamine Oxidase; D010624:Phenelzine