Physicochemical properties of avian tracheal mucus.
Dual-radiolabelled avian tracheal secretions were obtained by giving Na235SO4 and D-[1-3H]glucosamine simultaneously into the lumen of the trachea in preparations in vitro. These secretions comprised fibrillar, gelatinous and soluble-phase mucins. These were eluted as single components in the non-retarded fractions from Bio-Gel A-15m. Although no evidence of the presence of subunit structure was found, chemical and radiolabelling analyses showed a high degree of internal inhomogeneity among the three types of mucins. The differences among these mucins could be attributed to the chemical nature of their constituent glycoproteins. Glycoprotein fractions separated by ion-exchange chromatography were found to contain sulphate and N-acetylneuraminic acid residues in differing amounts. The overall acidic properties appeared to be correlated with ester sulphate content. A close similarity in the carbohydrate composition and a reciprocal relationship between the total ester sulphate residue contents and 35S- and 3H-labelling suggested that, in addition to stepwise glycosylation and sulphation, some pre-existing sulphated oligosaccharides might have been utilized for the synthesis of acidic glycoproteins.
D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D005260:Female; D006023:Glycoproteins / Q000032:analysis; D008297:Male; D009077:Mucins / Q000302:isolation & purification / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D014132:Trachea / Q000378:metabolism*; D014316:Tritium; D014783:Viscosity
D002241:Carbohydrates; D006023:Glycoproteins; D009077:Mucins; D013462:Sulfur Radioisotopes; D014316:Tritium