Selected ion monitoring of apomorphine in whole rat brain.
Mass fragmentography was used for quantitation of apomorphine in whole rat brain. Following extraction with 0.45 N perchloric acid, the components were derivatized to the O,O-bis(trimethylsilyl) (TMS) derivatives with N,O-bis(trimethylsilyl)trifluoroacetamide at 30 degrees C. The molecular ions of the TMS derivatives of apomorphine (m/z 411) and N-n-propylnorapomorphine (m/z 439), as an internal standard, were assayed simultaneously by selected ion monitoring. Total chromatography time is 5 min. By this method 50 ng/ml can be determined with a coefficient of variation of 12.0%. Application of the method to apomorphine disposition study in whole rat brain was demonstrated.
D000818:Animals; D001058:Apomorphine / Q000032:analysis*; D001923:Brain Chemistry; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D014297:Trimethylsilyl Compounds
D014297:Trimethylsilyl Compounds; D001058:Apomorphine