[Effect of pulsed laser radiation on the lysosomes of Ehrlich ascitic tumor cells].
The results of a study on the effect of neodymium pulsed laser radiation on Ehrlich ascites tumor lysosomes are discussed. Tumor cell suspension was exposed in vitro to 10 pulses of laser irradiation with fluences of 10, 25 and 100 J/cm2, at a wavelength of 1.06 microns and a pulse duration of 1 microsecond. A rise in the free and total activities of acid phosphatase in the fraction of isolated lysosomes was registered one or four hours after exposure, particularly, at fluences of 10 and 25 J/cm2. However, laser radiation did not affect acid phosphatase activity in preparations of solubilized enzyme. Special experiments involving the use of detergents showed the increased activity of acid phosphatase in isolated lysosome fraction to be due to labilization of cytoplasmic membranes of tumor cells.
D000135:Acid Phosphatase / Q000528:radiation effects; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology / Q000601:surgery*; D002462:Cell Membrane / Q000201:enzymology / Q000528:radiation effects; D005260:Female; D005609:Free Radicals; D066298:In Vitro Techniques; D053685:Laser Therapy; D008247:Lysosomes / Q000201:enzymology / Q000528:radiation effects*; D051379:Mice; D008815:Mice, Inbred Strains
D005609:Free Radicals; D000135:Acid Phosphatase
Vliianie impul'snogo lazzernogo izlucheniia na lizosomy kletok astsitnoĭ opukholi Erlikha.