Macrophage phagocytosis: analysis of particle binding and internalization.
An improved radioassay for analysis of phagocytosis has been used to quantitate and characterize the binding and internalization of zymosan by monolayers of rabbit pulmonary alveolar macrophages. This method distinguishes zymosan particles reversibly bound to the cell surface from those internalized. The zymosan was radiolabeled with technetium 99m(99mTc), a gamma-emitter with a 6-h half-life. Use of 99mTc as the radiolabel also permitted simultaneous determinations of pinocytosis and cellular protein content using [14C]sucrose and [3H]amino acids, respectively. All endocytic data were normalized per adherent cell based on this latter measurement. A significant fraction of the cell-associated particles was bound to the cell surface but not internalized. Failure to correct for this compartment would have resulted in overestimation of phagocytic rate and total cellular capacity. Both binding and ingestion of functionally unopsonized zymosan were found to be saturable, temperature sensitive, dependent on glycolytic energy, dependent on a trypsin-sensitive membrane component, described by a maximal rate, and limited by a finite capacity. The time courses of both processes were found to be similar. These results led us to conclude that, in our system, both binding and internalization were active processes and that the limited capacity to ingest zymosan was not explained by a concomitant reduction in binding of the particle to the cell membrane. Furthermore, it was found that phagocytosis did not change the rate of fluid-phase pinocytosis, consistent with the concept that the cell membrane is a functional mosaic as has been previously found by others for phagocytic and transport sites in this cell type.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism / Q000502:physiology*; D008297:Male; D008722:Methods; D010587:Phagocytosis; D010873:Pinocytosis; D011650:Pulmonary Alveoli / Q000166:cytology; D011817:Rabbits; D015054:Zymosan / Q000378:metabolism
D000596:Amino Acids; D015054:Zymosan