Energy and misonidazole toxicity: the effects of 5-thio-D-glucose.
Cell inactivation and DNA damage (single-strand breaks) were used to study the effects of inhibitors of anaerobic glucose oxidation on the toxicity of misonidazole to hypoxic Chinese hamster cells. Citrate and 2-deoxyglucose produced no effects on the toxicity. 5-thio-D-glucose (5-TG) protected cells of the CH2B2 line to some extent (SSB decreased by about 30%). In the CHO lines used (wild, and ethylmethanesulfonate-sensitive mutants), 5-TG had varied effects. Non-protein sulfhydryl (NPSH) levels were measured in all lines. Cells with lower NPSH levels are more sensitive to misonidazole; these are the cells which are protected by 5-TG. Cell line variations must be considered when studying interactions between a drug and other forms of treatment as possible treatments of cancer.
D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004347:Drug Interactions; D004734:Energy Metabolism / Q000187:drug effects*; D005947:Glucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D008920:Misonidazole / Q000633:toxicity*; D009593:Nitroimidazoles / Q000633:toxicity*; D013438:Sulfhydryl Compounds / Q000378:metabolism
D000963:Antimetabolites; D009593:Nitroimidazoles; D013438:Sulfhydryl Compounds; C003002:5-thio-D-glucose; D008920:Misonidazole; D005947:Glucose