[Isolation of preparative amounts of influenza virus neuraminidase by affinity chromatography].
Synthesis of neuraminidase activity in an affinity column with an inhibitor para-amino-phenyloxamine acid (PAPOA), immobilized on sepharose via tripeptide "spacer" (glycyl-glycyl-tyrosine) is described as well as the conditions of PAPOA synthesis. The column has been tested for isolation of neuraminidase types N1 and N2 from various influenza virus strains. The strain specificity of the action of the column in neuraminidase isolation was demonstrated. Neuraminidase free from admixtures has been isolated from the A/PR8/34, A/FM/1/47 and recombinant X-7 strains.
D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D009980:Influenza A virus / Q000201:enzymology*; D008854:Microscopy, Electron; D009439:Neuraminidase / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D014771:Virion / Q000201:enzymology
D009439:Neuraminidase
Vydelenie preparativnykh kolichestv neĭraminidazy virusa grippa metodom affinnoi khromatografii.