Characterization of the forms of bovine liver adenosine deaminase.
1. The A and C forms of bovine liver adenosine deaminase (adenosine aminohydrolase; EC 3.5,4.4) have been separated. 2. The proportion of two forms is dependent on ionic strength of solution. 3. By gel filtration, in presence of 6 M urea, and A form is dissociated into the C form and the binding factor and both are also separated. By removal of urea the A form is again obtained. 4. The molecular weights of two forms and binding factor, kinetic parameters have been determined.
D000243:Adenosine Deaminase / Q000145:classification* / Q000302:isolation & purification; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D007527:Isoenzymes / Q000302:isolation & purification; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D009700:Nucleoside Deaminases / Q000145:classification*
D007527:Isoenzymes; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase