Structure of adenovirus chromatin.
The structure of adenovirus type 2 chromatin isolated from wild-type and ts1 virions was investigated by micrococcal nuclease digestion and electron microscopy. Partial digestion of wild-type and ts1 chromatin with micrococcal nuclease generated a multimeric DNA smear devoid of the 200 basepair nucleosome repeating pattern characteristic of cellular chromatin. However, 11 S monomer cores of 150 basepairs were detectable. The chromatin of ts1 (39 degrees C) was more resistant to digestion by micrococcal nuclease. Two-dimensional electrophoresis of the monomer core showed that wild-type core contained protein VII while ts1 (39 degrees C) core contained PVI and PVII. Protein V appears to be located on the variable-length intermonomer region. Crosslinking studies suggest that proteins PVII and VII exist in dimeric form within the monomer core. Electron microscopy revealed a 5.5-fold-condensed two-micron-long beaded structure with about 200 monomer particles spaced irregularly. Based on these observations, a model for adenovirus prochromatin and chromatin is proposed that differs in important aspects from the model proposed previously (Corden, J., Engelking, H.M. and Pearson, G.D. (1976) Proc. Natl. Acad. Sci. USA 73, 401-404).
D000260:Adenoviruses, Human / Q000235:genetics*; D002499:Centrifugation, Density Gradient; D002843:Chromatin / Q000648:ultrastructure*; D004279:DNA, Viral / Q000032:analysis; D004586:Electrophoresis; D008836:Micrococcal Nuclease; D008854:Microscopy, Electron; D009707:Nucleosomes / Q000648:ultrastructure*; D014764:Viral Proteins / Q000032:analysis
D002843:Chromatin; D004279:DNA, Viral; D009707:Nucleosomes; D014764:Viral Proteins; D008836:Micrococcal Nuclease