Development of cytotoxic cells during in vitro cultures of pleural effusion lymphocytes from patients with lung cancer.
Culture-induced cytotoxic (CIC) activity developed in 7-day culture in the presence of fetal calf serum of pleural effusion lymphocytes from patients with lung cancer which initially showed markedly low or no natural killing (NK) activity against K562 cells. The development of CIC activity was proliferation-dependent. The CIC-mediating effector cells were removed by nylon-wool adherence, whereas the NK cells were enriched. Precursors for cytotoxic cells were not clarified. These results suggest that the precursors for cytotoxic cells are present in carcinomatous pleural effusions and able to develop cytotoxicity after 7-day culture in vitro.
D000328:Adult; D000368:Aged; D000920:Antibody-Dependent Cell Cytotoxicity; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural / Q000032:analysis*; D008175:Lung Neoplasms / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010996:Pleural Effusion / Q000032:analysis*