Actin organization and fibrin-clot retractile activity of cultured mouse fibroblasts.
It is known that human and animal fibroblasts are able to induce the retraction of a fibrin clot. In the present study the correlation between (i) fibrin-fibroblasts during growth, (ii) the number of actin stress-lines in mouse fibroblasts during growth in culture, and (iii) the sensitivity of actin stress-lines to a powerful actin-depolymerizing factor (ADF), present in plasma and serum of humans and laboratory animals was investigated. Fibroblasts at early passages (2-4) were tested for these parameters at various intervals after seeding (24, 96, and 168 h). The number of actin stress-lines was progressively higher, while the sensitivity to ADF action was progressively lower in cells cultured from 24 to 168 h; the FCR capacity was significantly decreased at 168 h. These data suggest that cells containing weakly polymerized and/or stabilized actin are more active than those containing highly polymerized and/or stabilized actin in triggering fibroblast contraction.
D051339:Actin Depolymerizing Factors; D000199:Actins / Q000502:physiology*; D000818:Animals; D001798:Blood Proteins / Q000502:physiology; D002478:Cells, Cultured; D051345:Destrin; D005260:Female; D005337:Fibrin / Q000502:physiology*; D005347:Fibroblasts / Q000648:ultrastructure; D051379:Mice; D008840:Microfilament Proteins; D011247:Pregnancy
D051339:Actin Depolymerizing Factors; D000199:Actins; D001798:Blood Proteins; C495824:DSTN protein, human; D051345:Destrin; C495825:Dstn protein, mouse; D008840:Microfilament Proteins; D005337:Fibrin