[Morphological changes in isolated metaphase chromosomes depending on the pH and ionic strength of the solutions].
The morphology of isolated unfixed Chinese chamster chromosomes is described in relation to the composition of solutions used for chromosome isolation. The chromosomal morphology was studied with the aid of a flow-chamber and phase-contrast microscopy. Changes in chromosome length and width in relation to pH values (ranging from 2.2 to 9.15) and ionic strength (ranging from 0 to 2.0 M) are detected.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002875:Chromosomes / Q000648:ultrastructure*; D006224:Cricetinae; D003412:Cricetulus; D006863:Hydrogen-Ion Concentration; D008677:Metaphase; D009994:Osmolar Concentration; D012996:Solutions
D012996:Solutions
Izmenenie morfologii vydelennykh metafaznykh khromosom v zavisimosti ot pH i ionnoĭ sily rastvorov.