Decreased levels of S-adenosylmethionine in the livers of rats fed phenobarbital and DDT.
Chronic feeding of the liver tumour promoters phenobarbital (PB) and 1,1-bis(rho-chlorophenyl)-2,2,2-trichloroethane (DDT) to male weanling rats at levels of 0.05% in the diet for 1, 3 and 5 weeks reduced the hepatic S-adenosylmethionine (AdoMet) contents by approximately 50%. Such reductions were inhibited by the simultaneous feeding of choline chloride and were completely prevented by the dietary administration of methionine. AdoMet levels greater than 100 mug/g liver were noted in rats receiving PB + methionine for 1 week, DDT + methionine for 1 week or methionine alone for 5 weeks. These results are consistent with the hypothesis that the liver tumour promoting activities of PB and DDT may occur in part through a hepatic methyl insufficiency.
D000818:Animals; D001835:Body Weight; D002794:Choline / Q000494:pharmacology; D003634:DDT / Q000494:pharmacology*; D004032:Diet; D004435:Eating; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008715:Methionine / Q000494:pharmacology; D009929:Organ Size; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D012436:S-Adenosylmethionine / Q000032:analysis* / Q000378:metabolism
D012436:S-Adenosylmethionine; D008715:Methionine; D003634:DDT; D002794:Choline; D010634:Phenobarbital