Titration of antiserum to South American rattlesnake (Crotalus durissus terrificus) venom by measuring inhibition of phospholipase A2 activity.
Horse antiserum to the venom of Crotalus durissus terrificus, A South American rattlesnake, inhibits the phospholipase activity of the crude venom. There is a close relationship between this inhibitory property and the neutralizing potency of the antiserum in vivo. This may provide the basis for a rigorous standardization of anticrotalid venom in vitro.
D000818:Animals; D010856:Columbidae; D003435:Crotalid Venoms / Q000276:immunology* / Q000633:toxicity; D006736:Horses / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology / Q000592:standards*; D008297:Male; D051379:Mice; D009500:Neutralization Tests; D010740:Phospholipases / Q000037:antagonists & inhibitors*; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D011817:Rabbits / Q000276:immunology
D003435:Crotalid Venoms; D007106:Immune Sera; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2