Establishment and characterization of a human myeloma cell line (KMM-1).
A new cell line (KMM-1) was established from a subcutaneous plasmacytoma of a 62-year-old male with multiple myeloma. Immunological studies indicated that cultured cells were derived from the same clone of myeloma cells in vivo: smeared cells were stained with fluorescein-conjugated globulin of antisera monospecific to lambda-chain, and lambda-chains in the cell extracts and in culture media were identical to the Bence-Jones protein found in the patient's urine. The cell line grew in suspension with prominent nucleoli and rough endoplasmic reticulum. Cells had the karyotype of 47, X, -Y, Iq+, -2, +t(1:2) (cen:cen), +7, 12q+, 14q+, +mar and carried no Epstein-Barr virus-determined nuclear antigen. Surface markers were as follows; E rosette (-), IgG Fc receptor (-), C3 receptor (-), S-Ig (+), TdT (-), asialo-GM1 (-). The reasons for the successful establishment of the myeloma cell line are discussed.
D001536:Bence Jones Protein / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D002871:Chromosome Banding; D006801:Humans; D007621:Karyotyping; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics / Q000276:immunology / Q000473:pathology* / Q000648:ultrastructure; D011947:Receptors, Antigen, B-Cell / Q000276:immunology; D011971:Receptors, Immunologic / Q000276:immunology; D012397:Rosette Formation
D011947:Receptors, Antigen, B-Cell; D011971:Receptors, Immunologic; D001536:Bence Jones Protein