Ultrasonically induced nuclear aberrations in an in vitro multicellular tumour system.
Chinese hamster V-79 cells, attached to an acoustically transparent membrane and EMT6/Ro mouse mammary sarcoma cells as small multicellular spheroids or in suspension, were exposed to therapeutic intensities of 1 MHz ultrasound (0-10 W/cm2) and examined for the production of atypical nuclear or chromosomal aberrations. In the monolayer configuration no abnormalities occurred. In multicell spheroids or suspension cells 3 classes of abnormalities were found: (1) pyknotic/irregular nuclei; (2) lysed nuclei with extruding nucleoplasm; and (3) agglomerations which have an irregular shape and "fused" morphology similar to that which has been reported for plant tissues (Cataldo et al, 1973).
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D008325:Mammary Neoplasms, Experimental; D051379:Mice; D012513:Sarcoma, Experimental; D013997:Time Factors; D014465:Ultrasonics / Q000009:adverse effects*