Alpha gamma-enolase in the rat: ontogeny and tissue distribution.
The rat brain enolases are dimers composed of alpha and gamma subunits. At pH 8.6 alpha gamma-enolase seemed to be stable, and no evidence was found for the possible formation of alpha gamma-enolase from alpha alpha-enolase and gamma gamma-enolase in the course of rat brain homogenization. During ontogeny of the rat forebrain, alpha gamma-enolase was formed before gamma gamma-enolase. The half-maximal specific concentrations were reached at postnatal days 14 and 23, respectively. The distribution of alpha gamma- and gamma gamma-enolase in various rat brain areas was also investigated. In all areas both forms were present. In neuroendocrine tissues alpha gamma-enolase was present at much higher concentration than gamma gamma-enolase. The ratio between gamma gamma-enolase and alpha gamma-enolase may be indicative of the degree of neuronal maturation, a conclusion further substantiated by the high ratio observed in cerebellum and the low ratio observed in olfactory bulbs, both compared with the ratio in forebrain.
D000375:Aging; D000818:Animals; D001921:Brain / Q000201:enzymology*; D004027:Diencephalon / Q000254:growth & development; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D007123:Immunoelectrophoresis, Two-Dimensional; D007527:Isoenzymes / Q000378:metabolism*; D046911:Macromolecular Substances; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013687:Telencephalon / Q000254:growth & development; D014018:Tissue Distribution
D007527:Isoenzymes; D046911:Macromolecular Substances; D010751:Phosphopyruvate Hydratase