[Thermoactivation of poly(G).poly(C): a description of the effect and its hypothetical mechanism].
Heating of poly(G).poly(C) complex solutions at a temperature about 100 degrees C was shown to overcome a decrease in the antiviral and interferon-inducing activity of the preparations which were obtained at relatively high concentrations of polynucleotides from poly(G) stored in solution, or were stored frozen themselves. These unfavourable conditions contributed to stabilization of the poly(g) secondary structure and decrease in the degree of regularity of the complex molecules. The results suggest that thermal activation of such poly (G). poly(C) preparations occurred in 2 stages by melting residual free regions of poly(G) and their subsequent interaction with poly(C) with formation of a more regular complex.
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D006358:Hot Temperature; D007369:Interferon Inducers / Q000494:pharmacology; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000188:drug therapy; D011066:Poly C / Q000494:pharmacology* / Q000627:therapeutic use; D011068:Poly G / Q000494:pharmacology* / Q000627:therapeutic use; D011131:Polyribonucleotides / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013997:Time Factors
D000998:Antiviral Agents; D007369:Interferon Inducers; D011131:Polyribonucleotides; D011068:Poly G; C008961:poly G-poly C; D011066:Poly C
Termoaktivatsiia poli(G).poli(Ts): opisanie éffekta i ego predpolagaemyĭ mekhanizm.