Cyclization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine to penicillins by cell-free extracts of Streptomyces clavuligerus.
Cell-free extracts prepared by sonication of Streptomyces clavuligerus cyclized delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) into a penicillin-type antibiotic. The antibacterial spectrum of this antibiotic suggested it was a mixture of isopenicillin N and penicillin N indicating that both cyclization and racemase activities were present. Cyclization activity was optimal in extracts prepared from 48 hours cultures. Extracts incubated at 20 degrees C produced antibiotic for 2 hours before activity ceased. Cyclization activity showed an absolute requirement for dithiothreitol (DTT) and O2 and was stimulated by ascorbic acid and FeSO4. No requirement for ATP was observed.
D002474:Cell-Free System; D004229:Dithiothreitol / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology; D010406:Penicillins / Q000096:biosynthesis*; D013302:Streptomyces / Q000378:metabolism*
D010406:Penicillins; D010100:Oxygen; D004229:Dithiothreitol