Protective effects of diisopropyl 1, 3-dithiol-2-ylidene malonate (NKK-105) on liver injury by carbon tetrachloride (CCl4).
Effects of NKK-105 against lipid peroxidation of microsomes by CCl4 are investigated, in vitro. NKK-105 had no effect on microsomal lipid peroxidation. With the microsomal fraction was preincubated with NKK-105 before adding CCl4, the lipid peroxidation induced with CCl4 was almost prevented by NKK-105. Effects of NKK-105 administration on aminopyrine N-demethylase, cyt.b5, cyt.p-450 and lipid peroxidation in CCl4-induced liver damage were investigated, in vivo. NKK-105 had no effect on the activity of aminopyrine N-demethylase and the content of cyt.b5, cyt.p-450 and peroxide. The content of cyt.b5, cyt.p-450 and the activity of demethylase remarkably decreased with CCl4 administration, but the content of cyt.p-450 and the demethylase activity were restored about 75% of the control values and cyt.b5 content was completely restored with previous administration of NKK-105. The amount of peroxide in the microsomal fraction increased with CCl4 administration was restored to the control level with previous administration of NKK-105.
D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000517:prevention & control*; D056486:Chemical and Drug Induced Liver Injury; D008054:Lipid Peroxides / Q000378:metabolism; D008107:Liver Diseases / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D008314:Malonates / Q000494:pharmacology*; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D008054:Lipid Peroxides; D008314:Malonates; C024808:diisopropyl 1,3-dithiol-2-ylidenemalonate