Enrichment by counterpart centrifugal elutriation of human lymphocytes cytotoxic to human tumour cells.
The enriched fractions of cytotoxic cells responsible for natural killer (NK) activity against both human sarcoma and neuroblastoma (LA-N2) cell lines were readily obtained by countercurrent centrifugal elutriation (CCE). The NK cells were obtained in the larger lymphocyte fractions (fraction 6 +/- 1), having a mean cell volume of 180 u3. The cytotoxic-enriched fraction contained 51% large lymphocytes having cytoplasmic granules. On the other hand, monocytes were purified to greater than 90% and isolated in another fraction (final fraction) and these cells had the lowest NK activity against both human tumour cell lines. However, compared with the lymphocyte fractions, small and large monocytes displayed greater antibody-dependent cellular cytotoxicity (ADCC) activity against human B erythrocytes. These results indicate that NK found to have activity against both tumour cells lines were larger lymphocytes, not small monocytes. Thus, countercurrent centrifugal elutriation (CCE) can provide a sensitive method to obtain enriched fractions of large lymphocytes contained tumoricidal activity against human sarcoma and neuroblastoma cell lines.
D000920:Antibody-Dependent Cell Cytotoxicity; D002460:Cell Line; D002469:Cell Separation; D002498:Centrifugation; D003594:Cytoplasmic Granules; D003602:Cytotoxicity, Immunologic; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007223:Infant; D007694:Killer Cells, Natural / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D009000:Monocytes / Q000276:immunology; D009369:Neoplasms / Q000276:immunology*; D009447:Neuroblastoma / Q000276:immunology; D012509:Sarcoma / Q000276:immunology