Heme biosynthesis in the heart.
The rates of biosynthesis of heme a and heme b in hearts of fed and fasted rats were measured using an isolated heart perfusion system. delta-Aminolevulinic acid synthetase activity was decreased in hearts of fasted rats to about 30% of values in hearts obtained from fed rats. [14C] Glycine incorporation into hemes a and b of cardiac tissue obtained from fasted rats was also decreased to about 30% of values obtained in hearts from fed rats. Cobalt addition to the perfusion fluid led to a decrease in cardiac delta-aminolevulinic acid synthetase activity just as cobalt administration to rats does in vivo. These studies strongly suggest that delta-aminolevulinic acid synthetase activity regulates the rate of synthesis of hemes a and b in the heart.
D000624:5-Aminolevulinate Synthetase / Q000032:analysis; D000622:Aminolevulinic Acid / Q000378:metabolism; D000818:Animals; D005998:Glycine / Q000378:metabolism; D006418:Heme / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D006418:Heme; D000622:Aminolevulinic Acid; D000624:5-Aminolevulinate Synthetase; D005998:Glycine