Microbicidal activity of bone marrow cells. Augmentation of Candida killing activity of the cells by culturing with fibroblast conditioned medium.
Mouse bone marrow cells were seeded in 96-well trays and infected with twofold serially diluted Candida parapsilosis. Outgrowth of the fungi in each well was determined after a 48-hr incubation period. Freshly collected cells demonstrated a candidacidal activity which increased with increase in the number of cells seeded. When fresh cells were cultivated with conditioned medium of mouse embryo fibroblasts, the candidacidal activity continuously increased for a few days and reached a plateau. The activity was augmented more than 1,000-fold compared with that of fresh marrow cells. It is suggested that augmentation is due not to the increase in number of effector cells but to the maturation of effector cells. Carrageenan, which is specifically cytotoxic to macrophages, inhibited augmentation of the activity. Therefore, it appears that most effector cells that matured by cultivation with the conditioned medium belong to the monocyte-macrophage lineage.
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology; D000818:Animals; D001854:Bone Marrow Cells; D002175:Candida / Q000254:growth & development*; D002351:Carrageenan / Q000494:pharmacology; D002478:Cells, Cultured; D003470:Culture Media; D005347:Fibroblasts; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000502:physiology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR
D003470:Culture Media; D008070:Lipopolysaccharides; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D002351:Carrageenan