Translational specificity in reovirus-infected mouse fibroblasts.
The specificity of protein synthesis in reovirus-infected mouse SC-1 cells was investigated, with the following results: a, extracts from infected and control cells translated saturating levels of capped globin mRNA with the same efficiency; b, infected cell extracts did not display a virus-induced increase in the capacity to translate decapped globin mRNA; c, translation of both cellular and viral messages was equally sensitive to the cap analog, 7-methylguanosine 5'-triphosphate. These findings are consistent with a previously published model (Walden, W., Godefroy-Colburn, T., and Thach, R. E. (1981) J. Biol. Chem. 256, 11739-11746) in which translation rates in reovirus-infected SC-1 cells are regulated by competition of capped host and viral mRNAs for a component of the unaltered protein synthetic system. Similar experimental results were obtained using reovirus-infected mouse L cells, with the following exceptions: a, the shutoff of host translation was far greater in L cells than in SC-1 cells; b, extracts of infected L cells were less active than controls for translation of all mRNAs tested. The reasons for the differences between SC-1 cells and L cells in their response to reovirus infection are being investigated.
D000818:Animals; D002460:Cell Line; D005347:Fibroblasts / Q000378:metabolism* / Q000382:microbiology; D005914:Globins / Q000096:biosynthesis; D007739:L Cells / Q000382:microbiology; D051379:Mice; D008954:Models, Biological; D014176:Protein Biosynthesis / Q000187:drug effects; D012314:RNA Cap Analogs / Q000494:pharmacology; D012315:RNA Caps / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D012087:Reoviridae / Q000502:physiology*; D014764:Viral Proteins / Q000096:biosynthesis
D012314:RNA Cap Analogs; D012315:RNA Caps; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; C017516:7-methylguanosine triphosphate; D005914:Globins