[Simple purification of acetonitrile for high performance liquid chromatography (HPLC) of peptides and proteins (author's transl)].
We report a simple method of purifying commercially available acetonitrile by column chromatography over aluminium oxide (neutral). The absorption at 220 nm or 230 nm is decreased to values (A = 0.03) which provide a constant baseline in high performance liquid chromatography: the analyses of proteins and peptides area easier.
D000097:Acetonitriles / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D010455:Peptides / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D000097:Acetonitriles; D010455:Peptides; D011506:Proteins; C032159:acetonitrile
Einfache Reinigung von Acetonitril für die hochdruckflüssigkeitschromatographie (HPLC) von Peptiden und Proteinen.