The alkaline hydrolysis of phosphotriesters in alkylated mammalian DNA.
Isolated DNA was alkylated with N-[14C]methyl-N-nitrosourea or N-[14C]ethyl-N-nitrosourea. Sedimentation analysis of the alkylated DNA before and after alkaline hydrolysis was used to determine the number of single-strand breaks introduced by hydrolysis of the triesters. Vacuum distillation from alkylated DNA solutions before and after alkaline hydrolysis was used to determine the numbers of triesters hydrolysing to the alcohol.
D000478:Alkylation; D000818:Animals; D002250:Carbon Radioisotopes; D004247:DNA / Q000378:metabolism*; D005038:Ethylnitrosourea / Q000378:metabolism*; D006868:Hydrolysis; D008099:Liver / Q000378:metabolism; D008770:Methylnitrosourea / Q000378:metabolism*; D051379:Mice; D009607:Nitrosourea Compounds / Q000378:metabolism*; D010755:Organophosphates; D009943:Organophosphorus Compounds
D002250:Carbon Radioisotopes; D009607:Nitrosourea Compounds; D010755:Organophosphates; D009943:Organophosphorus Compounds; D008770:Methylnitrosourea; D004247:DNA; D005038:Ethylnitrosourea