Verapamil and calcium-stimulated enzyme efflux from skeletal muscle.
An early and significant biochemical abnormality in Duchenne's muscular dystrophy is an increase in intracellular calcium. We have found that the "calcium-blocker" drug verapamil inhibits calcium-stimulated efflux of creatine kinase (EC 2.7.3.2) and lactate dehydrogenase (EC 1.1.1.27) in vitro from normal human skeletal muscle at therapeutic concentrations. Such a calcium blocker might therefore be useful in the treatment of Duchenne's muscular dystrophy.
D002118:Calcium / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D003402:Creatine Kinase / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D009132:Muscles / Q000187:drug effects* / Q000201:enzymology; D009136:Muscular Dystrophies / Q000201:enzymology; D014700:Verapamil / Q000494:pharmacology*
D014700:Verapamil; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D002118:Calcium