Effects of dietary paraffin, squalane and sucrose polyester on residue disposition and elimination of hexachlorobenzene in rats.
Previous studies have shown addition of light liquid paraffin to enhance the elimination of organochlorine xenobiotics. In the present study the effect of paraffin on the elimination of [14C]hexachlorobenzene (HCB) was compared with the effect of possible alternative compounds, squalane and sucrose polyester (SPE). Four groups of 7 rats were fed a diet containing 1.5 ppm [14C]HCB for 4 days followed by 10 days on HCB-free diet. Thereafter one group (control) remained on this diet whereas the other 3 groups received a diet supplemented with 8% (w/w) paraffin, squalane or SPE, respectively. Radioactivity in urine and faeces was measured daily and at the end of the experiment in samples of abdominal fat, muscle, liver, kidney and blood. Dietary treatment with either paraffin, squalane or SPE markedly enhanced faecal excretion of [14C]HCB, whereas urinary excretion was not affected. Both the time course as well as the extent of faecal [14C]HCB elimination were similar in the treated groups. After 3 weeks of treatment the amount of [14C]HCB excreted with faeces was about three times higher in treated animals than in controls. The half-life (t1/2) of [14C]HCB elimination from the body was markedly decreased in treated animals (mean 34--38 days) compared to controls (110 days). [14C]HCB concentrations in some major tissues were significantly reduced to the same extent by all three dietary regimens. Thus squalane and SPE are as effective as paraffin in removing HCB from contaminated animals.
D000273:Adipose Tissue / Q000032:analysis; D000818:Animals; D002722:Chlorobenzenes / Q000378:metabolism*; D004032:Diet; D005227:Fatty Acids; D005243:Feces / Q000032:analysis; D006581:Hexachlorobenzene / Q000378:metabolism*; D007668:Kidney / Q000032:analysis; D008297:Male; D009132:Muscles / Q000032:analysis; D010232:Paraffin / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013185:Squalene / Q000031:analogs & derivatives* / Q000494:pharmacology; D013395:Sucrose / Q000031:analogs & derivatives* / Q000494:pharmacology; D014018:Tissue Distribution
D002722:Chlorobenzenes; D005227:Fatty Acids; D006581:Hexachlorobenzene; D013395:Sucrose; C013692:sucrose polyester; D013185:Squalene; D010232:Paraffin; C019556:squalane