Production of autoantibodies by human-human hybridomas.
Peripheral blood lymphocytes and splenocytes of patients with autoimmune disease were used to prepare human-human hybridomas that produce autoantibodies. Because exogenous immunization was not used, the hybridoma antibodies were derived from B cells that spontaneously produced autoantibodies. 108 hybrids grew from 4,254 wells (2.5%). Optimal conditions for obtaining hybridomas with the GM 4672 myeloma line included initial growth in 2-ml wells, the use of 44% polyethylene glycol, a mononuclear cell/GM 4672 cell ratio 5:1, and prior stimulation of the B lymphocytes with pokeweed mitogen. Hybridoma supernatants had activity against ssDNA, platelets, and erythrocytes. The results demonstrate the feasibility of producing human-human hybridomas from lymphocytes of patients with various autoimmune diseases.
D000744:Anemia, Hemolytic, Autoimmune / Q000276:immunology; D001323:Autoantibodies / Q000096:biosynthesis*; D001327:Autoimmune Diseases / Q000276:immunology; D001666:Binding Sites, Antibody; D001792:Blood Platelets / Q000276:immunology / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology
D001323:Autoantibodies; D004277:DNA, Single-Stranded