Biological activity of a lipopolysaccharide extracted from Treponema hyodysenteriae.
A lipopolysaccharide (LPS) was obtained from pathogenic Treponema hyodysenteriae by hot phenol-water extraction. Various effects of the LPS on host cells were examined in vitro. Toxicity for mouse peritoneal macrophages was observed after 10 h of incubation at concentrations as low as 15 micrograms of the LPS per ml. Marked enhancement of both complement (C3) and immunoglobulin G-Fc receptor-mediated internalization was noted in macrophages obtained from mice injected 6 days previously with 75 micrograms of the material. Incorporation of [3H]thymidine into murine splenocytes was elevated approximately fourfold when splenocytes were treated with 5 to 10 micrograms of LPS per ml. Incubation of the LPS with normal porcine serum resulted in the generation of a factor(s) that stimulated the migration of porcine leukocytes. Generation of the chemotactic activity was inhibited by heating the serum at 56 degrees C for 30 min before treatment with LPS. The results suggest that T. hyodysenteriae contains an LPS that is biologically active.
D000818:Animals; D001202:Ascitic Fluid; D002448:Cell Adhesion; D002455:Cell Division; D002630:Chemotactic Factors / Q000097:blood*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008934:Mitogens / Q000494:pharmacology*; D010587:Phagocytosis; D013552:Swine; D014208:Treponema / Q000032:analysis*
D002630:Chemotactic Factors; D008070:Lipopolysaccharides; D008934:Mitogens