A new method for isolation of saprophytic cultures of Claviceps fusiformis from sclerotia.
The proposed method includes primary and secondary treatments. Of the various disinfectants tested, 1-propanol and 1-butanol as a primary treatment and phenol, resorcinol, HgC12 or NaCl as a secondary treatment completely eliminated the contamination hazard. 1-Butanol and phenol were not useful disinfectants since they inhibited the growth of C. fusiformis along with the other contaminants. Primary and secondary treatment of sclerotia with 1-propanol and resorcinol, respectively, produced the maximum of stable cultures. Out of 120 cultures tested, 5 cultures demonstrated an appreciable yield of alkaloids under submerged cultural conditions.
D000433:1-Propanol / Q000494:pharmacology; D002967:Claviceps / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D004202:Disinfectants / Q000494:pharmacology; D004876:Ergot Alkaloids / Q000096:biosynthesis; D008722:Methods; D012118:Resorcinols / Q000494:pharmacology
D004202:Disinfectants; D004876:Ergot Alkaloids; D012118:Resorcinols; D000433:1-Propanol