Fluoresceinated lectins as probe for cell surface changes associated with lymphocyte transformation.
Lectins are plant-derived proteins with specific affinities for simple or complex sugars and are potentially useful tools for investigation of the carbohydrate structure of the cell surface. A panel of fluoresceinated lectins was used to study the cell surface changes associated with lymphocyte transformation. The major determinants detected on peripheral blood lymphocytes were alpha D-mannose, beta-D-galactose and N-acetyl-glucosamine. Phytohemagglutinin transformation was associated with increased binding of lectins specific for N-acetyl-galactosamine, MOLT-4, a T-lymphoblast cell line in continuous culture, was associated with increased binding of lectins specific for N-acetyl-galactosamine and L-fucose. Application of fluoresceinated lectins may be a useful tool in the classification of lymphoid cells.
D050260:Carbohydrate Metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D005452:Fluoresceins; D006801:Humans; D037102:Lectins; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism* / Q000648:ultrastructure; D009369:Neoplasms / Q000378:metabolism
D005452:Fluoresceins; D037102:Lectins