[A non-damaging procedure for DNA isolation from insect cell nuclei].
A gentle procedure is described for DNA isolation from Galleria mellonella cell nuclei. Nuclei lysis was performed by SDS in final concentration of 3% which was followed by DNA extraction from chromatin by a stepwise rise of NaCl concentration up to 2.5 M. After removal of nuclei insoluble components by centrifugation the native DNA was purified using the hydroxyapatite column. The method proposed provides a higher yield of the native DNA as compared with the phenol-detergent method.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis*; D004247:DNA / Q000302:isolation & purification*; D007915:Lepidoptera / Q000648:ultrastructure*; D008722:Methods; D009036:Moths / Q000648:ultrastructure*
D004247:DNA
Shchadiashchiĭ metod vydeleniia DNK iz iader kletok nasekomykh.