Structures of N-linked and O-linked oligosaccharides on proteoglycan monomer isolated from the Swarm rat chondrosarcoma.
Monomer proteoglycans isolated from the Swarm rat chondrosarcoma contain O- and N-glycosidically linked oligosaccharides. The O-glycosidically linked ones were released from the protein core by alkaline borohydride treatment. After removal of sialic acid, the resulting oligosaccharide alditols were converted to N-trifluoroacetyl derivatives by trifluoroacetolysis. The N-trifluoroacetylated oligosaccharide alditols show shorter retention time on gas-liquid chromatography, as permethylated derivatives, than the corresponding N-acetyl derivatives. The mass spectra of these new derivatives also give more structural information and provide definitive structures. Alkaline borohydride treatment of the proteoglycans also released N-glycosidically linked oligosaccharide-peptides. These were purified and analyzed by Smith degradation, trifluoroacetolysis, and chromium trioxide oxidation. The results indicate that more than 70% of the N-glycosidically linked oligosaccharides have the following structure: (formula, see text) with the Fuc present on only about 60% of the oligosaccharides.
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002813:Chondrosarcoma / Q000032:analysis*; D002850:Chromatography, Gel; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D009844:Oligosaccharides / Q000032:analysis*; D011509:Proteoglycans / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D012513:Sarcoma, Experimental / Q000032:analysis*
D007202:Indicators and Reagents; D009844:Oligosaccharides; D011509:Proteoglycans