Preparation and antigenic properties of estriol 16-glucuronide- and estradiol 17-glucuronide-[C-6]-bovine serum albumin conjugates.
The preparation and antigenic properties of estriol 16-glucuronide-bovine serum albumin (BSA) and estradiol 17-glucuronide-BSA conjugates in which the hapten molecule is linked to the carrier protein through an (O-carboxymethyl)oxime bridge at the C-6 position on the steroid nucleus, have been described. Antibodies raised against the two immunogens in the rabbit possessed excellent specificity to estriol 16-glucuronide and estradiol 17-glucuronide, respectively, exhibiting no significant cross-reactions with estrogen glucuronides having a substituent in ring A and no cross-reactivities with free estrogens, their sulfates and related steroids.
D000906:Antibodies / Q000276:immunology; D003429:Cross Reactions; D004958:Estradiol / Q000031:analogs & derivatives* / Q000276:immunology; D004964:Estriol / Q000031:analogs & derivatives* / Q000276:immunology; D012709:Serum Albumin / Q000276:immunology*
D000906:Antibodies; D012709:Serum Albumin; C025483:estradiol-17 beta-glucuronide; C012961:estriol-16 alpha-(beta-D-glucuronide); D004958:Estradiol; D004964:Estriol