Measurement of the fasting urinary hydroxyproline: creatinine ratio in normal adults and its variation with age and sex.
Conditions for the determination of the fasting urinary hydroxyproline: creatinine ratio (OHPr: Cr ratio) have been examined, using a resin-catalysed hydrolysis and automated colorimetric procedure for the determination of hydroxyproline. Feeding experiments with gelatin showed that hydroxyproline is rapidly absorbed and excreted in the urine and that a 12-hour (overnight) fast is sufficient to ensure correct and reproducible fasting OHPr: Cr ratios. The mean fasting OHPr: Cr ratio decreased slightly with increasing age in both men and premenopausal women but there was no significant difference between the sexes. There was, however, a significant increase in the mean ratio in postmenopausal women. The normal range of fasting OHPr: Cr ratios for men and premenopausal women was found to be 0.003--0.015.
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D003404:Creatinine / Q000652:urine*; D005215:Fasting; D005260:Female; D006801:Humans; D006909:Hydroxyproline / Q000652:urine*; D008297:Male; D008593:Menopause; D008875:Middle Aged; D012737:Sex Factors
D003404:Creatinine; D006909:Hydroxyproline