Ca2+- and calmodulin-dependent protein phosphorylation in rat lacrimal gland.
Ca2+, in homogenized lacrimal glands, enhanced phosphorylation of several peptides. Phosphorylation of two of these peptides was further stimulated by addition of the Ca2+-binding protein calmodulin and decreased by trifluoperazine, an inhibitor of Ca2+--calmodulin-dependent activity. Thus, Ca2+--calmodulin-dependent protein kinases and their substrates are present in lacrimal gland and could have an important role in lacrimal-gland function.
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D002147:Calmodulin / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000378:metabolism*; D066298:In Vitro Techniques; D007765:Lacrimal Apparatus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical; D014268:Trifluoperazine / Q000494:pharmacology
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D005136:Eye Proteins; D014268:Trifluoperazine; D002118:Calcium