[Reaction between 125I-collagen and growing fibronectin polypeptides of chick embryo polyribosomes].
Polyribosomes from chicken embryos and 125I-collagen were shown to form complexes, which might be isolated from free 125I-collagen by means of centrifugation. The fraction of rapidly sedimenting polyribosomes was the most active in formation of complexes with 125I-collagen. The complexes ribosomes-125I-collagen appeared after destruction of polyribosomes-125I-collagen complexes by means of RNAase. Treatment of the complexes with puromycine led to liberation of about 40% of 125I-collagen. Antibodies against fibronectin inhibited the complex formation by about 30%. The data obtained suggest that the growing fibronectin polypeptides participate in formation of at least 30% of the complexes.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D003094:Collagen / Q000378:metabolism*; D005353:Fibronectins / Q000096:biosynthesis / Q000378:metabolism*; D066298:In Vitro Techniques; D010455:Peptides / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism*; D011691:Puromycin / Q000494:pharmacology; D012260:Ribonucleases / Q000494:pharmacology
D005353:Fibronectins; D010455:Peptides; D011691:Puromycin; D003094:Collagen; D012260:Ribonucleases
Vzaimodeĭstvie 125I-kollagena s rastushchimi polipeptidami fibronektina polirobosm kurinykh émbrionov.