Immunological cross-reactivity between the alpha-bungarotoxin-binding component from rat brain and nicotinic acetylcholine receptor.
An alpha-bungarotoxin-binding protein was partially purified from rat brain and, when complexed with [125I] alpha-bungarotoxin, was shown to behave as a single radiolabelled protein that is distinct from the similarly complexed nAChR from Torpedo marmorata. The alpha-bungarotoxin-binding protein was used as antigen in radioimmunoassays for rabbit anti-(rat muscle nAChR) and rabbit anti-(Torpedo nAChR) antibodies, giving titres approximately 5% and 0.5%, respectively, of those obtained by using homologous antigen in the same assay.
D000818:Animals; D001921:Brain; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D003429:Cross Reactions; D007106:Immune Sera; D007526:Isoelectric Point; D009132:Muscles; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D011950:Receptors, Cholinergic / Q000276:immunology* / Q000302:isolation & purification; D011978:Receptors, Nicotinic; D014101:Torpedo; D064569:alpha7 Nicotinic Acetylcholine Receptor
C579165:Chrna7 protein, rat; D007106:Immune Sera; D011950:Receptors, Cholinergic; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor