High-performance liquid chromatography of human serum lipoproteins: selective detection of triglycerides by enzymatic reaction.
A simple and convenient method for detection and quantitation of triglycerides in each lipoprotein fraction (chylomicron, VLDL, LDL and HDL) has been developed by high-performance liquid chromatography followed by enzymatic reaction using a high-speed reaction type chromatograph. Triglycerides in serum lipoproteins eluted from the gel permeation column (TSK GEL) could be sensitively and selectively detected by the absorbance at 550 nm using a commercial reagent kit. The distribution of triglycerides in each lipoprotein fraction could be examined with a small amount of serum (10-50 microliters) in less than 50 min by this method. Moreover, free glycerol could be detected as a sharp peak at the elution volume of total permeation of the column. This technique was found to be suitable for the study of triglyceride-rich lipoproteins such as chylomicron and VLDL.
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002914:Chylomicrons / Q000097:blood; D005215:Fasting; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008079:Lipoproteins, VLDL / Q000097:blood; D012016:Reference Values; D014280:Triglycerides / Q000097:blood*
D002914:Chylomicrons; D008074:Lipoproteins; D008079:Lipoproteins, VLDL; D014280:Triglycerides