Transacylation as a chain-termination mechanism in fatty acid synthesis by mammalian fatty acid synthetase. Synthesis of medium-chain-length (C8-C12) acyl-CoA esters by goat mammary-gland fatty acid synthetase.
1. Ruminant mammary-gland fatty acid synthetases can, in contrast with non-ruminant mammary enzymes, synthesize medium-chain fatty acids. 2. Medium-chain fatty acids are only synthesized in the presence of a fatty acid-removing system such as albumin, beta-lactoglobulin or methylated cyclodextrin. 3. The short- and medium-chain fatty acids synthesized were released as acyl-CoA esters from the fatty acid synthetase.
D000214:Acyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000494:pharmacology; D004952:Esters; D064429:Fatty Acid Synthases / Q000378:metabolism*; D005227:Fatty Acids / Q000096:biosynthesis*; D005260:Female; D006041:Goats; D006168:Guinea Pigs; D007774:Lactation; D007782:Lactoglobulins / Q000494:pharmacology; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D011247:Pregnancy; D011817:Rabbits; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D012756:Sheep
D000214:Acyl Coenzyme A; D003505:Cyclodextrins; D004952:Esters; D005227:Fatty Acids; D007782:Lactoglobulins; D012710:Serum Albumin, Bovine; D064429:Fatty Acid Synthases