Crystallization and preliminary x-ray data for a glycosylated form of bovine pancreatic ribonuclease.
A glycosylated form of bovine pancreatic ribonuclease, ribonuclease B, has been crystallized in a form suitable for three-dimensional x-ray structure determination. Crystals grown from polyethylene glycol solutions display high resolution diffraction patterns indicating the orthorhombic space group P2(1)2(1)2(1) with a = 59.2 (+/- 0.1), b = 56.0 (+/- 0.1), and c = 81.0 (+/- 0.2). A, V = 2.7 x 10(5) A3. With 8 molecules/unit cell Vm = 2.28 A3/dalton. The systemically weak intensity of all reflections with their l indices odd suggests the likely alignment of a noncrystallographic 2-fold axis near the crystal c axis.
D000818:Animals; D050260:Carbohydrate Metabolism; D002417:Cattle; D003460:Crystallization; D012260:Ribonucleases / Q000378:metabolism; D014961:X-Ray Diffraction
D012260:Ribonucleases; C029600:ribonuclease B