An immunoglobulin V gene polymorphism in the rat.
Anti-phenyloxazolone (phOx) antibodies of different AVN rats (primary response) share several isoelectric focusing bands. These bands were not shared by antibodies of some other rat strains, including DA. An anti-idiotype reagent was prepared (in rabbits) that bound radioactive anti-phOx antibodies of AVN rats but not normal AVN immunoglobulin. This binding was strongly inhibited by AVN anti-phOx antisera, but not by AVN anti-BOC-p-azobenzene arsonate-tyrosine antisera or DA anti-phOx antisera. Anti-phOx antisera of (AVN X DA)F1 rats were also strongly inhibitory indicating the presence of the idiotype (Ox-r1). Antisera of backcross rats (AVN X DA) X DA either resembled F1 hybrid sera (31 rats) or DA sera (23 rats). The data suggest that the presence of idiotype Ox-r1 is controlled by one gene, or genes linked to each other. The gene(s) is not linked to the Ig kappa chain locus. It may be a V gene of the Ig H chain.
D000818:Animals; D000906:Antibodies / Q000235:genetics / Q000276:immunology; D003433:Crosses, Genetic; D005260:Female; D005796:Genes; D007114:Immunization; D007122:Immunoelectrophoresis; D007130:Immunoglobulin Idiotypes / Q000235:genetics*; D008297:Male; D010081:Oxazolone / Q000031:analogs & derivatives / Q000276:immunology; D011110:Polymorphism, Genetic; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics*
D000906:Antibodies; D007130:Immunoglobulin Idiotypes; C018361:2-phenyloxazolone; D010081:Oxazolone