Analysis of picomolar concentrations of 6-oxo-prostaglandin F1 alpha in biological fluids.
A highly sensitive and specific assay for the quantitation of 6-oxo-prostaglandin F1 alpha, the stable hydrolysis product of prostacyclin, is described. The method involves the addition of [3,3',4,4'-2H4]-6-oxo-prostaglandin F1 alpha as internal standard, extraction from biological fluids using muBondapak C18 reversed-phase Sep-Paks, and preliminary purification by normal-phase chromatography. Following conversion to the methoxime, tris-trimethylsilyl, pentafluorobenzyl derivative, samples were analysed using combined capillary column gas chromatography negative ion chemical ionisation mass spectrometry. Fragments ions at m/z 614 (1H) and 618 (2H) [M-C7H2F5]- were monitored for quantitation. This method was used for the measurement of endogenous levels of 6-oxo-prostaglandin F1 alpha in human urine and for the determination of prostacyclin release from rat peritoneal mast cells and from rat aortic rings incubated in human plasma.
D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D011453:Prostaglandins / Q000378:metabolism; D011460:Prostaglandins F / Q000652:urine*; D051381:Rats; D012016:Reference Values
D011453:Prostaglandins; D011460:Prostaglandins F; C100006:prostaglandin F1