Trapping of DNA-reactive metabolites of therapeutic or carcinogenic agents by carbon-14-labeled synthetic polynucleotides.
Many substances which do not react with DNA directly are metabolized into important DNA-modifying intermediates. We have devised a method for trapping these intermediates with 14C-labeled nucleosides contained in a synthetic polynucleotide. The polynucleotide structure protects the labeled nucleoside from metabolism; thus, it is unaltered when the polymer is incubated with a drug-metabolizing system. However, when the polymer is incubated with this system and a compound which can be metabolized into a reactive species, these intermediates are trapped by the 14C-labeled nucleoside and subsequently are detected as new peaks of radioactivity in a digest of the labeled polynucleotide. This system has been used to detect reactive intermediates of cyclophosphamide generated by a liver homogenate.
D000818:Animals; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid; D003520:Cyclophosphamide / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D008862:Microsomes, Liver / Q000378:metabolism*; D010752:Phosphoramide Mustards / Q000378:metabolism*; D011131:Polyribonucleotides / Q000378:metabolism*
D002250:Carbon Radioisotopes; D010752:Phosphoramide Mustards; D011131:Polyribonucleotides; C030090:phosphoramide mustard; D003520:Cyclophosphamide; D004247:DNA