Cytoplasts can transfer factor(s) that stimulate quiescent fibroblasts to enter S phase.
Cytoplasts prepared from fibroblasts arrested by hydroxyurea were fused with serum-arrested, quiescent fibroblasts. In contrast to unfused mono-nucleated cells in the same culture, a sizable fraction of these cybridoids entered S phase in the absence of extracellular serum stimulation. Because DNA synthesis commenced only after a considerable lag following fusion, it was concluded that the cytoplasts contain factors which initiate the progression of quiescent cells toward S phase.
D000818:Animals; D001769:Blood; D002459:Cell Fusion; D002460:Cell Line; D006224:Cricetinae; D003593:Cytoplasm / Q000502:physiology*; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts; D006822:Hybrid Cells; D007399:Interphase; D051379:Mice; D013696:Temperature; D013997:Time Factors
D004247:DNA