Photolabeling of brain membrane proteins by lysergic acid diethylamide.
3H-Lysergic acid diethylamide (3H-LSD) is irreversibly incorporated into bovine caudate membranes during ultraviolet light illumination. The incorporated radioligand apparently forms a covalent bond with a subpopulation of the membrane proteins. Although the photolabeling pattern differs significantly from the Coomassie blue staining pattern on SDS gels, the photolabeling is apparently not specific for LSD binding sites associated with neurotransmitter receptors. 3H-LSD photolabeling can occur during prolonged exposure of membrane samples to room lighting and thus may introduce artifacts into receptor binding assays.
D000345:Affinity Labels; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D008238:Lysergic Acid Diethylamide / Q000494:pharmacology*; D008565:Membrane Proteins / Q000378:metabolism* / Q000528:radiation effects; D011954:Receptors, Dopamine / Q000378:metabolism; D011985:Receptors, Serotonin / Q000378:metabolism; D014466:Ultraviolet Rays
D000345:Affinity Labels; D008565:Membrane Proteins; D011954:Receptors, Dopamine; D011985:Receptors, Serotonin; D008238:Lysergic Acid Diethylamide