Deformability and osmotic fragility of phenylhydrazine-injected rat erythrocytes fractionated by Percoll density-gradients.
Red blood cells (RBCs) from phenylhydrazine-injected rats were separated according to their specific densities by centrifugation on a polyvinyl-pyrrolidone-coated colloidal silica matrix (Percoll). The deformability and osmotic fragility of fractionated RBCs were measured by ektacytometer and coil planet centrifuge, respectively. Immature reticulocytes, the least dense RBC subpopulation, were less deformable and osmotically more resistant than normal RBCs. Heinz-body containing RBC, the most dense subpopulation, showed reduced deformability and increased osmotic fragility. These findings suggest that young reticulocytes have increased membrane stiffness and the Heinz-body containing RBCs have an extremely rigid membrane.
D000740:Anemia / Q000097:blood; D000818:Animals; D001696:Biomechanical Phenomena; D002458:Cell Fractionation / Q000379:methods; D002499:Centrifugation, Density Gradient; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D006366:Heinz Bodies / Q000648:ultrastructure; D008297:Male; D009996:Osmotic Fragility / Q000187:drug effects; D010659:Phenylhydrazines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013314:Stress, Mechanical
D010659:Phenylhydrazines; C030299:phenylhydrazine