Potent in vivo and in vitro prolactin inhibiting activity of sauvagine, a frog skin peptide.
The effect of sauvagine (SAU), a frog skin peptide, on prolactin (PRL) levels was studied in vivo and in vitro. Subcutaneous administration of SAU (20 micrograms/kg) reduced plasma PRL levels in normal adult male rats and suppressed the suckling-induced rise of PRL in lactating rats even at doses of 1 and 5 micrograms/kg. Perfusion of isolated and dispersed rat pituitary cells in vitro with increasing doses of SAU (from 5 x 10(-10) to 1.7 x 10(-8)M) induced a significant dose-related decrease of PRL secretion in the eluate. These results indicate that SAU is a potent PRL inhibiting factor and that its action is exerted at the pituitary level. If SAU or a SAU-related peptide is present in the mammalian brain, it can be tentatively hypothesized that this peptide plays an important role in the control of PRL secretion.
D029845:Amphibian Proteins; D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D008297:Male; D036361:Peptide Hormones; D010455:Peptides / Q000494:pharmacology*; D010477:Perfusion; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism; D011388:Prolactin / Q000037:antagonists & inhibitors*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D029845:Amphibian Proteins; D036361:Peptide Hormones; D010455:Peptides; D001971:Bromocriptine; C025727:sauvagine; D011388:Prolactin; D004298:Dopamine