The application of sodium deoxycholate and Sephacryl-200 for the delipidation and separation of high density lipoprotein.
A method to remove lipids from human plasma high-density lipoprotein (HDL) was developed. The procedure required column chromatography on Sephacryl-200 in the presence of the bile salt sodium deoxycholate. The lipid free protein obtained retained the immunological properties of the native HDL.
D000180:Acrylic Resins; D001053:Apolipoproteins / Q000097:blood / Q000302:isolation & purification; D001054:Apolipoproteins A; D003840:Deoxycholic Acid; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood* / Q000302:isolation & purification
D000180:Acrylic Resins; D001053:Apolipoproteins; D001054:Apolipoproteins A; D008075:Lipoproteins, HDL; D003840:Deoxycholic Acid; C023049:sephacryl S 200