Influences on the expression of acetylcholine receptors on rat nodose neurones in cell culture.
1. Nodose neurones dissociated from new-born rats were grown in culture in the absence or presence of cells from neonatal skeletal muscle or heart. 2. In cultures devoid of non-neuronal cells cholinergic interactions between the neurones were common. In the presence of non-neuronal cells such interactions were rare. 3. A decrease in the proportion of neurones responsive to ACh was primarily responsible for the reduced incidence of synaptic interactions. Non-neuronal cells influenced the expression of ACh receptors in developing nodose neurones in culture. 4. Most neurones appeared susceptible to the non-neuronal influence during the first week in culture. 5. Many nodose ganglion neurones, whether grown in the presence or absence of non-neuronal cells, were sensitive to gamma-aminobutyric acid and serotonin but were insensitive to glutamate, glycine and L-epinephrine.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D008564:Membrane Potentials / Q000187:drug effects; D009132:Muscles / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009620:Nodose Ganglion / Q000254:growth & development / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D011950:Receptors, Cholinergic / Q000502:physiology*; D012701:Serotonin / Q000494:pharmacology; D013569:Synapses / Q000502:physiology; D014630:Vagus Nerve / Q000502:physiology*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D011950:Receptors, Cholinergic; D012701:Serotonin; D005680:gamma-Aminobutyric Acid; D000109:Acetylcholine