Unique cell surface glycoprotein expression in hairy cell leukemia: effect of phorbol ester tumor promoters on surface glycoproteins, cell morphology, and adherence.
Hairy cell leukemia cells from eight different patients exhibited a characteristic cell surface glycoprotein pattern when labeled by the neuraminidase-galactose oxidase-NaB3H4 procedure. The diffuse high-molecular-weight glycoprotein band (Mr 230,000 to 300,000) was not seen in other leukemic cell types and may represent a specific hairy cell leukemia antigen. Hairy cell leukemia cells can be maintained as cell suspension cultures, but treatment with a variety of tumor-promoting phorbol esters caused the cells to adhere to plates, assume a fibroblastic elongated shape, and extend long processes. This dramatic morphological change was not associated with any change in surface glycoproteins.
D000328:Adult; D000368:Aged; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007938:Leukemia / Q000032:analysis; D007943:Leukemia, Hairy Cell / Q000032:analysis* / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis*; D010703:Phorbol Esters / Q000494:pharmacology*; D010704:Phorbols / Q000494:pharmacology*; D055728:Primary Myelofibrosis / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D006023:Glycoproteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D010703:Phorbol Esters; D010704:Phorbols; D013755:Tetradecanoylphorbol Acetate