Precursor-size components are the basic collagenous subunits of murine tumor basement membrane.
The acid-insoluble fraction of murine tumor basement membrane produced by the EHS sarcoma was extracted following reduction of disulfide bonds under non-denaturing conditions. Extracts prepared from tumors grown in either lathyritic or normal animals contain two procollagen-like chains as well as cross-linked oligomers of these chains. Extracted monomeric chains behave almost exactly like reduced, radiolabeled EHS collagen precursor chains on SDS-PAGE and SDS-agarose suggesting that they are very similar collagens. Since the collagen extracted after reduction represented a significant proportion of the total tumor collagen, it is likely that tumor basement membrane is assembled from precursor-size Type IV collagen molecules.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001485:Basement Membrane / Q000032:analysis*; D002850:Chromatography, Gel; D003094:Collagen / Q000032:analysis*; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007842:Lathyrism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight
D000596:Amino Acids; D003094:Collagen