Norepinephrine-induced elevation of acetyl coenzyme A in rat pineal glands in culture.
A micromethod for the determination of acetyl coenzyme A was developed and the relationship between the concentration of acetyl coenzyme A and the activity of rat pineal serotonin N-acetyltransferase was studied. Acetyl coenzyme A was determined by converting it into N-acetylserotonin using rat liver serotonin N-acetyltransferase. Subsequently, the hydroxy group of N-acetylserotonin was O-methylated by hydroxyindole-O-methyltransferase and S-[methyl-3H]adenosyl-l-methionine to form [3H]melatonin, which was then conveniently separated from S-[methyl-3H]adenosyl-l-methionine by thin-layer chromatography. The amount of radioactivity in melatonin is a measure of acetyl coenzyme A concentration. This method is sensitive and specific, since it can detect as low as 5 pmol of acetyl coenzyme A but not structurally related substances such as coenzyme A, adenosine diphosphate, cysteamine, D-panthothenic acid, or sodium acetate. After treating cultured rat pineal glands with l-norepinephrine (10 microM) for 6 hr, the concentration of acetyl coenzyme A was increased significantly from 3.26 +/- 0.37 to 10.24 +/- 0.93 pmol/gland, while the activity of serotonin N-acetyltransferase increased 68-fold. This result suggests that acetyl coenzyme A may play an important role in the norepinephrine-induced induction of serotonin N-acetyltransferase. Sensitivity and adaptability of this method can be utilized to measure acetyl coenzyme A in discrete regions of rat brain and in experimental conditions in which micromeasurement of acetyl coenzyme A may be required.
D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D001191:Arylamine N-Acetyltransferase / Q000378:metabolism; D008550:Melatonin / Q000096:biosynthesis; D008745:Methylation; D009638:Norepinephrine / Q000494:pharmacology*; D009924:Organ Culture Techniques; D010870:Pineal Gland / Q000187:drug effects / Q000378:metabolism*; D051381:Rats
D000105:Acetyl Coenzyme A; D001191:Arylamine N-Acetyltransferase; D008550:Melatonin; D009638:Norepinephrine