Catabolic half-lives and antigenic relationships of native, altered and commercially prepared human albumins in rabbits.
As determined in rabbits, the in vivo plasma survival, i.e. half-life (t 1/2), of albumin produced by the cold-ethanol procedure did not differ significantly from that of native albumin (p less than 0.5). Plasma survivals of reprocessed and heat-ethanol produced albumins were significantly less than that of the native protein (0.02 less than p less than 0.05). Chemically-modified albumin had a considerably reduced t 1/2 (p less than 0.001) and was the only albumin shown to be antigenically different from the native protein. Variations in t 1/2 s of albumin preparations could not be attributed to differences in their polymer contents.
D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000276:immunology*; D000431:Ethanol / Q000494:pharmacology; D006207:Half-Life; D006358:Hot Temperature; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D046911:Macromolecular Substances; D011309:Preservation, Biological / Q000379:methods; D011817:Rabbits; D012709:Serum Albumin / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*
D000941:Antigens; D007106:Immune Sera; D046911:Macromolecular Substances; D012709:Serum Albumin; D000431:Ethanol