Separate detection of glucose-6-phosphate dehydrogenase from 6-phosphogluconate dehydrogenase by DEAE-paper chromatography.
Red blood cell lysates were applied to DEAE cellulose paper for detection of glucose-6-phosphate dehydrogenase. Glucose-6-phosphate dehydrogenase (G-6-PD) was separated from 6-phosphogluconate dehydrogenase (6-PGD) by DEAE-cellulose paper-chromatography and their activities were detected on the chromatogram by applying the reaction mixture. The fluorescence of NADPH formed by G-6-PD was not interfered with by 6-PGD activity. This is a sensitive procedure which detects slight loss of G-6-PD in red blood cells and could be applied to the detection of G-6-PD deficiency.
D002848:Chromatography, DEAE-Cellulose; D002854:Chromatography, Paper; D004912:Erythrocytes / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000097:blood / Q000302:isolation & purification*; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000175:diagnosis; D006801:Humans; D010734:Phosphogluconate Dehydrogenase / Q000097:blood / Q000302:isolation & purification*
D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase