[Effect of streptomycin on antigenic properties of serum beta-lipoproteins].
The antigenic properties of streptomycin compounds with heterologic beta-lipoproteids (beta-LP) of the donor blood serum were studied. beta-LP were isolated and then mixed with streptomycin sulfate in a dose of 10,000 and 50,000 micrograms/ml. 30 rabbits were immunized with such compounds. The control animals were treated with solutions of beta-LP and streptomycin alone. THe antisera collected from the animals were used in the reaction of precipitation and immunoelectrophoresis. Some physico-chemical properties of the preparations, such as pH and electrokinetic characteristics were studied. The comparative study with the use of the precipitation reaction showed that the immunochemical characteristics of heterologic beta-LP changed under the effect of streptomycin: the precipitation bands of the experimental antisera to the compounds of streptomycin with beta-LP differed from those of the control antisera to beta-LP in the quantitative and qualitative characteristics; in particular in the experiments with the antisera depletion a separate antigen was detected in beta-LP which formed under the effect of the antibiotic. The characteristic features of the reaction of precipitation were confirmed on the immunoelectrophoregrams. Addition of streptomycin to the solution of beta-LP induced no significant changes in pH of the compounds and their electrophoretic properties. The studies showed that the compounds of streptomycin with beta-LP had pronounced antigenic properties and their immunochemical characteristics significantly changed under the effect of the antibiotic.
D000818:Animals; D000941:Antigens / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008077:Lipoproteins, LDL / Q000097:blood / Q000276:immunology*; D011233:Precipitin Tests; D011234:Precipitins / Q000032:analysis; D011817:Rabbits; D013307:Streptomycin / Q000494:pharmacology*
D000941:Antigens; D007106:Immune Sera; D008077:Lipoproteins, LDL; D011234:Precipitins; D013307:Streptomycin
Vliianie streptomitsina na antigennye svoĭstva syvorotochnykh beta-lipoproteidov.