In vitro assay for phototoxic chemicals.
The photosensitizing potential of chemicals known to produce photosensitivity in humans was compared to chemicals not considered to be photosensitizers in an in vitro assay. The assay involved exposure of human lymphoid cells to UVA (320-400 nm), and in some cases UVB (280-320 nm) radiation, in the presence of the chemicals and the assessement of phototoxicity as measured by the incorporation of 3[H]-thymidine into nuclear DNA. All known photosensitizers tested were found to be phototoxic, while the nonphotosensitizing agents, with the exception of retinoic acid, were not phototoxic. Peripheral blood mononuclear cells were compared to a T lymphoblastoid cell line as target cells; the latter were superior in terms of convenience, cost and reproducibility of results. This test system has potential as a predictive assay for detecting additional phototoxic chemicals.
D002478:Cells, Cultured; D002746:Chlorpromazine / Q000009:adverse effects; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D008730:Methoxsalen / Q000009:adverse effects; D010787:Photosensitivity Disorders / Q000209:etiology*; D013936:Thymidine / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
D002746:Chlorpromazine; D008730:Methoxsalen; D013936:Thymidine