[Trypsin inhibitor from Gleditsia triacanthos L. seeds].
The trypsin inhibitor from Gleditsia triacanthos (L.) seeds was purified by affinity chromatography on a column with trypsin-Sepharose 4B. The isolated inhibitor is a single-chain protein with molecular weight of about 20 000. The inhibitor suppresses bovine trypsin at a molar rate of 1 : 1, but weakly inhibits chymotrypsin in a non-stoichiometric manner. Some properties of the isolated inhibitor closely resembled those of soybean trypsin inhibitor (Kunitz).
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D007700:Kinetics; D008970:Molecular Weight; D012639:Seeds / Q000032:analysis*; D014357:Trypsin / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000302:isolation & purification*
D014361:Trypsin Inhibitors; D002918:Chymotrypsin; D014357:Trypsin
Ingibitor tripsina iz semian gledichii (Gleditsia triacanthos L.).