Optimum conditions for growth and longevity of chick embryo fibroblasts in culture.
Chick embryo fibroblasts are often used as a model of cellular ageing in vitro. It seems important to culture these cells in optimum conditions which may not be the same as those for mammalian cells. An incubation temperature of 40 degrees C and the use of chicken serum were found to be optimum for growth and longevity of chick embryo fibroblasts in culture.
D000818:Animals; D001769:Blood; D002470:Cell Survival; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D005347:Fibroblasts / Q000166:cytology; D013696:Temperature
D003470:Culture Media