Tetracycline-induced mutation in cultured Chinese hamster cells.
After tetracycline hydrochloride (TC) treatment for 48 h, we obtained 8-azaguanine-resistant mutants in a population of cultured Chinese hamster cells. Optimal expression of mutation was reached after about 5 days, and mutation frequency remained constant thereafter. There was a gradual increase in the yield of mutants with increasing doses of TC, and measurable yield was observed at a dose as low as 10 microgram of TC per ml. When the cells, before or after the TC treatment, were grown in presence of L-ascorbic acid (10 or 20 microgram/ml) for 48 h, there was significant decrease in the frequency of mutation induced by TC. If L-ascorbic acid were present at the same concentration during the treatment with TC, no significant influence on the induction of mutants by TC was detected.
D000818:Animals; D001375:Azaguanine / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D004351:Drug Resistance; D007700:Kinetics; D008168:Lung; D009154:Mutation; D013752:Tetracycline / Q000494:pharmacology*
D003470:Culture Media; D013752:Tetracycline; D001375:Azaguanine