Determination of bradykinin, kallidin and Met-Lys-bradykinin by high performance liquid chromatography.
A high performance liquid chromatography system was developed for the quantitative separation and determination of bradykinin, kallidin and Met-Lys-bradykinin using a reversed-phase column (Nucleosil 5 C8) and an isocratic buffer system. The lower limit of detection is 20 pmol for bradykinin. The intra-variation coefficient of the assay is between 1.8 and 5.7%. The recovery of bradykinin is higher than 96%. Addition of human serum albumin to the assay system in a concentration of 40% lead to a remarkable reduction of the detectable kinin level, which indicates an adsorption of kinins to serum albumin.
D000595:Amino Acid Sequence; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007609:Kallidin / Q000302:isolation & purification*; D008832:Microchemistry; D012709:Serum Albumin
D012709:Serum Albumin; D007609:Kallidin; C018103:bradykinin, Met-Lys-; D001920:Bradykinin