Measurement of O6-ethyldeoxyguanosine and N-(deoxyguanosin-8-yl)-N-acetyl-2-aminofluorene in DNA by high-sensitive enzyme immunoassays.
Antibodies raised in rabbits against the bovine serum albumin conjugates of O6-ethylguanosine and N-(guanosin-8-yl)-N-acetyl-2-aminofluorene have been used to develop a high-sensitive enzyme-linked immunosorbent assay (HS-ELISA) for the quantification of adducts in DNA modified by ethylating agents and N-acetyl-2-aminofluorene. Linear dose-response relations were obtained in the non-competitive HS-ELISA between 0.5 fmol and 50 fmol O6-ethyldeoxy-guanosine per 2.8 microgram DNA, and between 0.1 fmol and 20 fmol N-(deoxyguanosin-8-yl)-N-acetyl-2-aminofluorene per 0.8 microgram DNA. The sensitivity of an ultrasensitive radioimmunoassay was in the same order of magnitude. Modification levels as low as 0.1 mumol of adduct/mol DNA-nucleotides (1: 10(7)) can be detected by each assay.
D015073:2-Acetylaminofluorene / Q000031:analogs & derivatives* / Q000032:analysis; D000818:Animals; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D011863:Radioimmunoassay; D012016:Reference Values
C018776:N-(deoxyguanosin-8-yl)acetylaminofluorene; C018534:O(6)-ethyl-2'-deoxyguanosine; D004247:DNA; D015073:2-Acetylaminofluorene; D003849:Deoxyguanosine