Specificity of ovine submaxillary-gland sialyltransferases. Application of high-pressure liquid chromatography in the identification of sialo-oligosaccharide products.
High-pressure liquid chromatography was used to identify the sialo-oligosaccharide products obtained after sialylation of [14C]Gal-GalNAc-protein in vitro by an ovine submaxillary-gland microsomal fraction. Among other products, two isomeric trisaccharides could be identified. NeuAc alpha 2 leads to 3Gal beta 1 leads to 3GalNAcol and Gal beta 1 leads to 3-(NeuAc alpha 2 leads to 6)GalNAcol respectively, indicating that ovine submaxillary gland contains two sialyltransferases acting on mucin-type acceptors, a beta-galactoside alpha 2 leads to 3 sialyltransferase and a N-acetylgalactosaminide alpha 2 leads to 6 sialyltransferase. This conclusion was fully supported by methylation analysis of the two trisaccharide products.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006023:Glycoproteins / Q000378:metabolism; D008745:Methylation; D008861:Microsomes / Q000201:enzymology; D009844:Oligosaccharides / Q000032:analysis*; D012756:Sheep; D012794:Sialic Acids / Q000032:analysis; D012799:Sialyltransferases / Q000378:metabolism; D013363:Submandibular Gland / Q000201:enzymology*; D014166:Transferases / Q000378:metabolism
D006023:Glycoproteins; D009844:Oligosaccharides; D012794:Sialic Acids; D014166:Transferases; D012799:Sialyltransferases