The presence of glutamate mutase in a methanol-utilizing bacterium, Protaminobacter ruber.
Cell-free extracts of a facultative methylotroph and strict aerobe, Protaminobacter ruber, could catalyze formation of beta-methylaspartate from glutamate. beta-Methylaspartate formed was further converted to mesaconate. From these results, it was found that the cells of P. ruber contained a sequential reaction system of glutamate mutase and beta-methylaspartase. The level of glutamate mutase activity was almost constant throughout the period of cultivation. When P. ruber was grown on several non-one-carbon compounds in addition to methanol as a sole carbon source, the activity of glutamate mutase was not markedly affected by the kinds of carbon sources.
D000591:Amino Acid Isomerases / Q000378:metabolism*; D000642:Ammonia-Lyases / Q000378:metabolism*; D001419:Bacteria / Q000201:enzymology* / Q000254:growth & development; D002474:Cell-Free System; D005632:Fructose / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D019751:Intramolecular Transferases; D000432:Methanol / Q000378:metabolism; D019946:Propylene Glycol; D011409:Propylene Glycols / Q000378:metabolism; D014805:Vitamin B 12 / Q000494:pharmacology
D005971:Glutamates; D011409:Propylene Glycols; D005632:Fructose; D019946:Propylene Glycol; D000642:Ammonia-Lyases; C034881:methylaspartate ammonia-lyase; D000591:Amino Acid Isomerases; D019751:Intramolecular Transferases; C021570:methylaspartate mutase; D014805:Vitamin B 12; D000432:Methanol