[In vitro hepatic cytochrome P-450 destruction induced by 1,2-dichloroethylene].
An estimation of the rate of 1,2-DCE metabolism by the hepatic microsomal cytochrome P-450 system was obtained by monitoring the oxidation of NADPH at 340 nm. During such a metabolism, cytochrome P-450 from PB-pretreated male mice is destroyed in vitro in presence of NADPH, after 30 minutes of incubation at 37 degrees C by 1,2-DCE. The NADPH-request suggests that oxidative metabolism of this olefin by cytochrome P-450 is necessary for the observed destruction. The loss of cyt.P-450 could be attributed to heme destruction, as the destructive interaction is always accompanied by a correlated loss of microsomal heme.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005025:Ethylene Dichlorides / Q000494:pharmacology*; D006843:Hydrocarbons, Chlorinated / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D009249:NADP / Q000378:metabolism; D013053:Spectrophotometry; D013997:Time Factors
D005025:Ethylene Dichlorides; D006843:Hydrocarbons, Chlorinated; D009249:NADP; C024565:ethylene dichloride; D003577:Cytochrome P-450 Enzyme System
Distruzione in vitro del citocromo P-450 epatico causata da 1,2-dicloroetilene.