[Rapid method for the identification of esterase-positive cells in suspensions of mononuclear cells separated by a Ficoll-Paque gradient].
Cytochemical staining for acid alpha-naphthyl acetate esterase (ANAE) activity is widely used for identifying monocytes in mixed cell populations; esterase staining is usually performed on slide prepartions of whole blood, buffy coat or Ficoll-Paque separated mononuclear cells (MC). In this paper we propose a method for staining MC while they are in suspension, which utilizes, with slight modifications, the same reagents used in the procedure for esterase staining on smears. Suspension method is rapid and costless; furthermore, it overcomes the loss of esterase stainability observed in slide preparations of Ficoll-Paque separated MC.U
D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D002469:Cell Separation / Q000379:methods; D006801:Humans; D009000:Monocytes / Q000166:cytology* / Q000201:enzymology; D009283:Naphthol AS D Esterase / Q000097:blood*; D013997:Time Factors
D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases
Metodo rapido per la identificazione delle cellule esterasi positive in sospensioni di cellule mononucleari ottenute su gradiente di Ficoll-Paque.