Massive heat-shock polypeptide synthesis in late chicken embryos: convenient system for study of protein synthesis in highly differentiated organisms.
In cultured eucaryotic cells, heat treatments specifically induced the rapid synthesis of the so-called heat-shock polypeptides. To ascertain the physiological importance of this phenomenon for highly differentiated organisms, we attempted to determine whether the heat-shock response occurs in a living endothermic organism at extreme temperatures, and if so, whether the response is organ specific. We developed a procedure to label proteins efficiently in 5- to 18-day-old chicken embryos. Heat-shock polypeptides of identical sizes of 85,000, 70,000, and 25,000 daltons were synthesized predominantly in chicken embryo fibroblasts and in many different organs of 18-day-old embryos at 42.5 to 44 degrees C.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D003609:Dactinomycin / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism; D006360:Heat-Shock Proteins; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008970:Molecular Weight; D009206:Myocardium / Q000378:metabolism; D014176:Protein Biosynthesis; D013696:Temperature
D006360:Heat-Shock Proteins; D003609:Dactinomycin