The measurement of theophylline metabolism in hepatic microsomes using high-performance liquid chromatography.
A method is described to measure the in vitro metabolism of theophylline by liver microsomes. The formation of 1-methyluric acid and 1,3-dimethyluric acid in incubation mixtures was determined by high-performance liquid chromatography. The formation of both metabolites was linear with time and the formation of 1-methyluric acid was blocked by allopurinol. This method will be useful in assessing potential drug interactions involving theophylline.
D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000378:metabolism*; D011817:Rabbits; D013806:Theophylline / Q000378:metabolism*
D013806:Theophylline