Immunoelectrophoretic characterization of human mitochondrial aspartate aminotransferase purified by ion exchange and affinity chromatography.
Human mitochondrial aspartate aminotransferase (m-ASAT) was prepared for use as an antigen for antibody production and as a standard, i.e. a high degree of purity was demanded. The purification was performed by three ion exchange chromatography steps followed by affinity chromatography on aspartate coupled gel. Four preparations gave specific activities of 230 to 300 U/mg at 37 degrees C. The purity of m-ASAT was assessed by crossed immunoelectrophoresis, which showed that the final preparation did not contain contaminating proteins. Immunization with the purified m-ASAT gave a good antibody response.
D001219:Aspartate Aminotransferases / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008930:Mitochondria, Liver / Q000201:enzymology*
D001219:Aspartate Aminotransferases