Thymidylate synthetase during synchronous nuclear division cycle and differentiation of Physarum polycephalum.
Thymidylate synthetase and thymidine kinase activities in wild type strain M3b and in thymidine kinase-deficient mutant TU63 of Physarum polycephalum are studied. Whenever nuclear division occurs in macroplasmodia of wild type, thymidine kinase and thymidylate synthetase activities sharply increase, although the increase of thymidylate synthetase activity is less pronounced than thymidine kinase activity. This is also true for other investigated nuclear divisions during the life cycle of P. polycephalum. It is shown for the first time that thymidylate synthetase is a periodically fluctuating enzyme during the naturally synchronous nuclear division cycle of P. polycephalum with a peak of specific activity in the S phase. In macroplasmodia, as well as after germination of microsclerotia of M3b, thymidine kinase is the dominant enzyme, whereas at the time of the precleavage mitosis in sporulating macroplasmodia thymidylate synthetase is the predominant enzyme. This study describes and compares both dTMP-synthesizing enzymes during proliferation and differentiation of the same organism.
D002453:Cell Cycle; D002467:Cell Nucleus / Q000502:physiology*; D007700:Kinetics; D008780:Methyltransferases / Q000378:metabolism*; D009154:Mutation; D010804:Physarum / Q000254:growth & development*; D013045:Species Specificity; D013937:Thymidine Kinase / Q000378:metabolism*; D013940:Thymidylate Synthase / Q000378:metabolism*
D008780:Methyltransferases; D013940:Thymidylate Synthase; D013937:Thymidine Kinase