A practical method for monitoring drug excretion and enterohepatic circulation in the rat.
An improved experimental design for studying the enterohepatic circulation of drugs in a pair of bile duct-duodenum cannula-linked rats is described. By housing the rats in individual metabolism cages and using a peristaltic pump to immediately deliver bile secreted by the donor directly into the duodenum of the recipient, the method permits a realistic approximation of biliary excretion and reabsorption in intact animals for periods up to 96 hours.
D000818:Animals; D001646:Bile / Q000378:metabolism; D004764:Enterohepatic Circulation; D008991:Monitoring, Physiologic / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D051381:Rats; D013693:Temazepam / Q000378:metabolism
D004364:Pharmaceutical Preparations; D013693:Temazepam