Estimation of lecithin in rabbit tissues.
1. A simple method for lecithin estimation was developed. Phospholipids in rabbit tissues were extracted with chloroform and methanol. 2. The samples were chromatographed with eluting solvent system; chloroform:methanol:glacial acetic acid:water (100:25:8:1.1). Lecithin was identified by the spray reagent as blue spots and the intensities were quantitated with a densitometer. 3. The concentration of lecithin in the various rabbit tissues varied from 2.8 to 10 mg/g tissue. The sequence of increasing lecithin concentration was heart less than kidney less than lung less than liver less than brain. 4. Recovery experiments of added lecithin into the tissues showed a recovery of 91-104%.
D000818:Animals; D002855:Chromatography, Thin Layer / Q000379:methods; D005260:Female; D007202:Indicators and Reagents; D008297:Male; D008982:Molybdenum; D010713:Phosphatidylcholines / Q000032:analysis*; D010743:Phospholipids / Q000302:isolation & purification; D011817:Rabbits
D007202:Indicators and Reagents; D010713:Phosphatidylcholines; D010743:Phospholipids; C017541:molybdenum blue; D008982:Molybdenum