Solid-phase immunoassays for quantitation of antibody to bovine white matter proteolipid apoprotein.
Two solid-phase immunoassays have been developed for quantitation of antibodies to bovine white matter proteolipid apoprotein. Conditions were established for optimal specific antibody binding. Water-soluble proteolipid apoprotein was bound to microtiter plates and plates were incubated with test serum. Goat anti-rabbit IgG conjugated with horseradish peroxidase was used as the second antibody for an enzyme-linked immunospecific assay and 125I-labeled protein A for a radioimmunoassay. Both procedures have been used to follow the time course of anti-proteolipid antibody production in rabbits and to compare different immunization protocols.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001059:Apoproteins / Q000032:analysis*; D001923:Brain Chemistry; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D006041:Goats / Q000276:immunology; D006735:Horseradish Peroxidase; D007118:Immunoassay / Q000379:methods; D007700:Kinetics; D011510:Proteolipids / Q000032:analysis*; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D013997:Time Factors
D000906:Antibodies; D001059:Apoproteins; D011510:Proteolipids; D006735:Horseradish Peroxidase