The distribution of thiol groups among the tryptic fragments of the heavy chain of myosin subfragment-1.
The limited tryptic digestion of the heavy chain of chymotryptic myosin subfragment-1 gives three major fragments (50 K, 27 K, and 20 K daltons) without much additional proteolysis. The distribution of thiols over the tryptic fragments of the heavy chain was estimated by sodium dodecyl sulfate-acrylamide gel electrophoresis. Contents of thiol in the 50 K, 27 K, and 20 K fragments were found to be 4, 1, and 4, respectively.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D009218:Myosins; D010446:Peptide Fragments / Q000032:analysis*; D011817:Rabbits; D013438:Sulfhydryl Compounds / Q000032:analysis*; D014357:Trypsin
D010446:Peptide Fragments; D013438:Sulfhydryl Compounds; D014357:Trypsin; D009218:Myosins