Studies on the liberation of fatty acids from 2-lysophosphatidylcholine by a liver lysosomal enzyme activity from chloroquine-treated rats.
A lysophospholipase activity was observed in cell fractions from rat liver without and after treatment with chloroquine following incubation with 1-[1(-14)C]palmitoyl-sn-glycero-3-phosphocholine. The specific activity of the enzyme (optimum pH at 4.2) is highly enriched in lysosomes but decreases after prolonged treatment of the animals with chloroquine. Some of the properties of the enzyme were studied.
D000818:Animals; D002738:Chloroquine / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008066:Lipolysis; D008099:Liver / Q000201:enzymology*; D008244:Lysophosphatidylcholines / Q000378:metabolism*; D008245:Lysophospholipase / Q000378:metabolism*; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology*; D010740:Phospholipases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D008244:Lysophosphatidylcholines; D002738:Chloroquine; D010740:Phospholipases; D008245:Lysophospholipase