Effect of lipid peroxidation on p-aminohippurate transport by rat kidney cortical slices.
1 The effect of lipid peroxidation on p-aminohippurate transport by rat kidney slices was examined. 2 Ascorbic acid and Fe2+ promoted lipid peroxidation of rat renal cortical slices in a dose-related manner. 3 Ascorbic acid (1.0 mM) and Fe2+ (0.4 mM) increased tissue water and decreased the accumulation of p-aminohippurate. 4 The addition of N,N'-diphenyl-p-phenylenediamine (antioxidant), at a concentration of 1 x 10(-6) M, completely inhibited the peroxidation and recovered the accumulation of p-aminohippurate. 5 The apparent Km of p-aminohippurate uptake was increased by ascorbic acid and Fe2+ with no change in the apparent V. 6 These data suggest that ascorbic acid and Fe2+ can cause a significant alteration in p-aminohippurate and water transport of renal cortical slices and that these effects can be correlated with lipid peroxidation.
D000618:Aminohippuric Acids / Q000378:metabolism*; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D005296:Ferrous Compounds / Q000494:pharmacology; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000378:metabolism*; D007700:Kinetics; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D010655:Phenylenediamines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D010130:p-Aminohippuric Acid / Q000378:metabolism*
D000618:Aminohippuric Acids; D005296:Ferrous Compounds; D008054:Lipid Peroxides; D010655:Phenylenediamines; C004845:N,N'-diphenyl-4-phenylenediamine; D001205:Ascorbic Acid; D010130:p-Aminohippuric Acid