[Use of preparative electrophoresis for isolating the light-chain hemagglutinin of the influenza virus].
A two-step method for isolation of HA2 polypeptide, the light chain of influenza virus hemagglutinin, from influenza virions is described. The method consists in obtaining a preparation of surface glycoproteins by means of their solubilization from virions with a cation detergent (cetyl-trimethyl-ammonium bromide) followed by fractionation of this preparation by preparative electrophoresis. Relative molecular weights of HA2 and other polypeptides comprising the virion have been determined. The method was used with a recombinant MRC-11 strain.
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006023:Glycoproteins / Q000302:isolation & purification; D006389:Hemagglutinins, Viral / Q000302:isolation & purification*; D009980:Influenza A virus / Q000276:immunology*; D010455:Peptides / Q000302:isolation & purification; D014771:Virion / Q000276:immunology
D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D010455:Peptides
Ispol'zovanie preparativnogo élektroforeza dlia izoliatsii legkoĭ tsepi gemaggliutinina virusa grippa.