Effect of selected immunoregulatory agents on low-grade contact sensitivity.
The effect of selected compounds with known immunoregulatory activity was examined in a 45-h sensitization period oxazolone contact-sensitivity reaction. Oxazolone sensitivity was induced by applying 0.1 ml of 5% oxazolone in absolute ethanol to the shaved abdomen of C57Bl/6 mice on day 0. Challenge with oxazolone followed 45 h later and was accomplished by painting a 5% solution of oxazolone in absolute ethanol on the left hindpaw. The response at 24 h was determined plethysmographically. Histamine (0.062-1.0 mg/kg, subcutaneously, twice a day), concanavalin A (0.31-5.0 mg/kg intravenously), penicillamine (6.25-25 mg/kg, subcutaneously), chloroquine (6.25-25 mg/kg, subcutaneously), and thymosin fraction 5 (0.125-1.25 mg/kg subcutaneously) all stimulated the oxazolone reaction when administered on day 0. These data suggest that the low-grade oxazolone response may be a useful assay to detect immunostimulatory activity.
D000276:Adjuvants, Immunologic / Q000276:immunology*; D000818:Animals; D002738:Chloroquine / Q000276:immunology; D003877:Dermatitis, Contact / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D006632:Histamine / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010081:Oxazolone / Q000276:immunology; D010396:Penicillamine / Q000276:immunology; D013947:Thymosin / Q000276:immunology
D000276:Adjuvants, Immunologic; D010081:Oxazolone; D013947:Thymosin; D006632:Histamine; D002738:Chloroquine; D010396:Penicillamine