The influence of adenosine on the no-reflow phenomenon in anoxic and ischaemic hearts.
Adenosine and the adenosine deaminase inhibitor erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA), separately and in combination, were added to the perfusate of isolated rat hearts which were then subjected to ischaemia or anoxia. The effect of these infusates on the vascular competence of the myocardium subjected to oxygen deprivation of from 15-90 min was compared to control hearts. Vascular competence at selected time intervals was assessed from the distribution of injected. 1% sodium fluorescein in the cut surface of the ventricles. A region of non-perfusion surrounding the left ventricular lumen and involving 25% of the ventricular myocardium developed within 15 min of anoxic perfusion, with little change thereafter. Adenosine had no significant effect on this. The no-reflow phenomenon following ischaemia had similar distribution, but developed more slowly and eventually involved twice as much of the myocardium (59% after 90 min). Surprisingly, pre-treatment with adenosine greatly increased (from 14-46%) the extent of no-reflow after 30 min of ischaemia. Pre-treatment with EHNA caused a slight reduction (59-43%) in its extent but only after 60 min. Thus the no-reflow phenomenon which developed in ischaemic myocardium is unlikely to be due to the reduced vasodilatory action of adenosine.
D000225:Adenine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D000241:Adenosine / Q000008:administration & dosage / Q000494:pharmacology*; D058892:Adenosine Deaminase Inhibitors; D000818:Animals; D003326:Coronary Circulation / Q000187:drug effects*; D003327:Coronary Disease / Q000473:pathology*; D004108:Dilatation, Pathologic; D007700:Kinetics; D008297:Male; D010100:Oxygen / Q000097:blood; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D058892:Adenosine Deaminase Inhibitors; C010192:9-(2-hydroxy-3-nonyl)adenine; D000225:Adenine; D000241:Adenosine; D010100:Oxygen