Acetaldehyde-induced lipid peroxidation in isolated hepatocytes.
In an effort to evaluate further the concept of ethanol-induced lipid peroxidation, isolated rat hepatocytes obtained via collagenase perfusion were utilized. Hepatocytes were judged to be functionally intact based on measurements of adenosine-5-triphosphate, gluconeogenesis, bromosulphthalein uptake, and trypan blue exclusion. When hepatocytes were incubated with acetaldehyde, a metabolite of ethanol, at 100 mg% and 10 mg%, significant increases in lipid peroxidation resulted as measured by levels of malonaldehyde. Acetaldehyde-induced increases in malonaldehyde were reduced by pre-incubation with antioxidants such as Vitamin E (200 mg%) or glutathione (100 mg%).
D000079:Acetaldehyde / Q000494:pharmacology* / Q000652:urine; D000085:Acetates / Q000652:urine; D000818:Animals; D000431:Ethanol / Q000494:pharmacology; D005260:Female; D005943:Gluconeogenesis / Q000187:drug effects; D008054:Lipid Peroxides / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000085:Acetates; D008054:Lipid Peroxides; D000431:Ethanol; D000079:Acetaldehyde