Microsomal bioactivation and covalent binding of aliphatic halides to DNA.
Studies were carried out on the in vitro covalent binding of a series of 14C-labeled aliphatic halides to calf thymus DNA following bioactivation by hepatic microsomes isolated from phenobarbital-treated rats. Six compounds were shown to exhibit binding to DNA of greater than 0.3 nmol/mg DNA (1,2-dibromoethane, bromotrichloromethane, trichloroethylene, carbon tetrachloride, chloroform, and 1,1,2-trichloroethane). Covalent binding of the aliphatic halides to the nucleic acids was confirmed by sedimentation of the DNA-organohalogen adduct in a cesium chloride gradient and Sephadex LH-20 chromatography of the nucleosides released by enzymatic hydrolysis.
D000818:Animals; D001711:Biotransformation; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography; D004247:DNA / Q000378:metabolism; D006846:Hydrocarbons, Halogenated / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats
D006846:Hydrocarbons, Halogenated; D004247:DNA