Ferrous- or cobalt ion-dependent D-(-)-tartrate dehydratase of pseudomonads: purification and properties.
D-(-)-Tartrate dehydratases [D-(-)-tartrate hydro-lyase, EC 4.2.1...] were isolated from two Pseudomonas strains. The molecular weights of the native enzymes were determined to be 72,000 and 7 8,000, respectively, and each enzyme was composed of two subunits of identical size. The dehydratases had no requirements for thiol compounds, were insensitive to oxygen, and required Fe2+ (0.1 mM) or Co2+ (0.5 mM) ions for optimal activity.
D003035:Cobalt / Q000494:pharmacology*; D005296:Ferrous Compounds / Q000494:pharmacology*; D006836:Hydro-Lyases / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology*; D007700:Kinetics; D008970:Molecular Weight; D010100:Oxygen / Q000494:pharmacology; D011549:Pseudomonas / Q000201:enzymology*; D013379:Substrate Specificity; D013644:Tartrates / Q000378:metabolism
D005296:Ferrous Compounds; D013644:Tartrates; D003035:Cobalt; D007501:Iron; C034710:D-(-)-tartrate dehydratase; D006836:Hydro-Lyases; D010100:Oxygen; C029768:tartaric acid