Fatty acid and acylglycerol glycerol synthesis in isolated liver cells from foetal lambs.
1. The rates of fatty acid and acylglycerol glycerol synthesis from acetate, glucose and L-lactate in isolated liver cells from foetal lambs at about 30 and 3 days before birth are reported. The total rates of fatty acid synthesis from endogenous precursors and added substrates, measured using 3H2O, are also reported for these liver cells. 2. The total rate of fatty acid synthesis did not differ at the two ages. Of the added substrates, acetate and L-lactate were better precursors for fatty acid synthesis than glucose under the conditions used. Endogenous precursors, probably glycogen glucose, made a substantial contribution to fatty acid synthesis. 3. Results are compared with previous studies of fatty acid synthesis in adipose tissue from foetal lambs.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D005227:Fatty Acids / Q000096:biosynthesis*; D005865:Gestational Age; D005947:Glucose / Q000378:metabolism; D005989:Glycerides / Q000096:biosynthesis*; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008099:Liver / Q000196:embryology* / Q000378:metabolism; D012756:Sheep / Q000196:embryology*
D000085:Acetates; D005227:Fatty Acids; D005989:Glycerides; D007773:Lactates; D019344:Lactic Acid; D005947:Glucose