Fusion and lipid exchange in vesicles containing lipophilic spin labels.
Lipophilic non-electrolyte spin labels greatly accelerate the fusion of unilamellar vesicles of dipalmitoylphosphatidylcholine when the system is maintained below the lipid phase transition. Differential scanning calorimetry and centrifugation measurements show that the transformed vesicles are large and probably unilamellar. Differential scanning calorimetry and fluorescence depolarization measurements were also carried out on mixtures of labeled dipalmitoylphosphatidylcholine vesicles and of vesicles composed of pure dimyristoylphosphatidylcholine. A mixing of the membrane components is observed when the vesicles are incubated above the transition temperature of the two constituent lipids. However, the process does not involve a real fusion of the entire vesicles. An exchange of lipid and label monomers between the two lipid phases seems to occur. These observations are discussed in view of the molecular organization of the spin label within the dipalmitoylphosphatidylcholine matrix below and above the lipid transition temperature.
D008081:Liposomes; D008561:Membrane Fusion; D008563:Membrane Lipids; D009391:Nephelometry and Turbidimetry; D010713:Phosphatidylcholines; D013113:Spin Labels; D013696:Temperature
D008081:Liposomes; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D013113:Spin Labels