Iodination with Iodo-gen and radioimmunoassay of cholecystokinin (CCK) in acidified plasma, CCK release, and molecular CCK components in man.
The preparation of a stable fully immunoreactive 125I-labeled CCK39 using a modified Iodo-gen method with high specific radioactivity; the production of an avid and specific cholecystokinin (CCK) antiserum, and a sensitive, precise and specific radioimmunoassay method allowing measurements of fasting plasma CCK in the low picomole per liter range together with the significant rises in plasma CCK following a test meal and duodenal infusion of fat are described. Apparent immunoreactive fasting plasma CCK was eluted from a Sephadex G-50 Fine column in one peak probably representing plasma CCK bound to plasma proteins and nonspecific plasma effects. Apparent immunoreactive postprandial plasma CCK was eluted from a Sephadex G-50 Fine column in four peaks. The first peak probably represents plasma CCK bound to plasma proteins and nonspecific plasma effects; the second peak probably represents component I with a molecular weight between some 5,000 and 30,000; the third peak probably represents component II or CCK33, and the fourth peak probably represents component IV or CCK8.
D000328:Adult; D000818:Animals; D002766:Cholecystokinin / Q000097:blood* / Q000276:immunology / Q000378:metabolism; D003314:Corn Oil; D004041:Dietary Fats / Q000008:administration & dosage; D004386:Duodenum / Q000378:metabolism; D006801:Humans; D007093:Imidazoles; D007106:Immune Sera; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D008297:Male; D009821:Oils / Q000008:administration & dosage; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*; D013552:Swine; D014508:Urea / Q000031:analogs & derivatives
D004041:Dietary Fats; D007093:Imidazoles; D007106:Immune Sera; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D009821:Oils; C012385:1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril; D003314:Corn Oil; D014508:Urea; D002766:Cholecystokinin