Tetrodotoxin resistant propagation and extracellular sodium changes during spreading depression in rat cerebellum.
Spreading depression (SD) was elicited in the benzoate-conditioned rat cerebellum by microinjection of KC1. Median SD propagation velocity was 9.2 mm . min-1. [Na+]0 decreases, median value 77.5 mM, were measured with ion-selective micropipettes. Superfusion of the cerebellum with 10-5 M tetrodotoxin did not modify the propagation velocity or decrease the [Na+]0 change. This confirms that classical voltage-mediated Na+ conductances are not involved in SD.
D000818:Animals; D002531:Cerebellum / Q000187:drug effects / Q000502:physiology*; D013181:Cortical Spreading Depression / Q000187:drug effects*; D005260:Female; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology*
D013779:Tetrodotoxin; D011189:Potassium Chloride; D012964:Sodium