Regulation of lymphocyte transformation responses to phytohaemagglutinin, concanavalin A or lipopolysaccharide by normal serum.
Lymphocyte blastogenesis of guinea-pig spleen cells induced by various doses of phytohaemagglutinin (PHA), concanavalin A (conA) or lipopolysaccharide (LPS) was assayed in the absence or in the presence of normal guinea-pig serum, foetal calf serum or heterologous albumin or gamma-globulins. Opposite effects of diluted and of concentrated sera were observed on blastogenesis induced either by PHA or by ConA: high concentrations inhibited blastogenesis whereas low concentrations enhanced mitogenesis. Homologous serum inhibited LPS-induced blastogenesis whatever the concentration used. These results could be explained by the interaction of serum with the membrane of lymphocytes stimulated by ConA, PHA or LPS. Furthermore sera could interfere with ConA. The implications of these experiments are discussed.
D000818:Animals; D001790:Blood Physiological Phenomena; D002417:Cattle; D002462:Cell Membrane / Q000276:immunology; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008934:Mitogens / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology
D008070:Lipopolysaccharides; D008934:Mitogens; D010835:Phytohemagglutinins; D003208:Concanavalin A