Nonpolar lipid methylation-identification of nonpolar methylated products synthesized by rat basophilic leukemia cells, retina and parotid.
Incorporation of radioactivity from [3H- or 14C-methyl] methionine into nonpolar lipids had been investigated in rat basophilic leukemia (RBL) cells, retina, and rat parotid gland. These nonpolar methylated lipids were extracted into heptane and characterized by thin-layer chromatography, high performance liquid chromatography, gas chromatography, and mass spectrometry. The major methylated nonpolar lipid product in the RBL cells themselves was ubiquinone-9, which accounted for about 90% of the nonpolar lipid and 20-30% of the total radioactive lipid formed. There was a modest increase in the methylation of nonpolar lipids upon stimulation of the RBL cells with IgE and anti-IgE, but the significance of this change is uncertain. In contrast to whole cells, RBL membrane fractions (incubated with [3H-methyl]-S-adenosylmethionine) incorporated radioactivity primarily into fatty acid methyl esters and not ubiquinone. A third product, 2-(methylthio)-benzothiazole, was formed by RBL cells, retina and minced parotid upon incubation in enriched media. This product was formed enzymatically, apparently by the known enzyme S-thiolmethyltransferase, using the thiol substrate which contaminates these media. Evidence suggest that the enzyme may reside, at least in part, on the surface of the cells.
D000818:Animals; D001491:Basophils / Q000378:metabolism*; D002460:Cell Line; D007938:Leukemia / Q000378:metabolism*; D050356:Lipid Metabolism; D008715:Methionine / Q000378:metabolism; D008745:Methylation; D008780:Methyltransferases / Q000502:physiology; D010306:Parotid Gland / Q000378:metabolism*; D051381:Rats; D012160:Retina / Q000378:metabolism*; D014451:Ubiquinone / Q000302:isolation & purification
D014451:Ubiquinone; D008715:Methionine; D008780:Methyltransferases