Interaction of phencyclidine with mouse neuroblastoma cells.
Growth of mouse neuroblastoma (Nb) cell (clone M1) was not affected by phencyclidine (PCP) concentrations of 10(-6)M up to 2 x 10(-4)M, whereas 10(-3)M PCP caused a 100% inhibition of cell growth. Several PCP analogs, including the quaternary PCP methiodide, exerted effects similar to those of PCP. The uptake of [piperidyl-3,4-3H]PCP ([3H]PCP) by the Nb cells was studied using cell monolayers in Petri dishes. Non-specific entry of PCP into the cells was linear with added substrate but specific uptake exhibited saturation kinetics. The concentration for half-maximum specific uptake was 2 x 10-(5)M, and the capacity of the cells at saturation was 2-3 nmoles [3H]PCP/mg protein, at 22 degrees. The uptake rate constant was 0.2 +/- 0.05 x 10(5) (M-1 min-1) and the dissociation constant was 0.25 +/- 0.05 (min-1). Uptake was temperature dependent and was inhibited by 2,4-dinitrophenol (DNP). This may indicate that this binding represents (at least in part) an active uptake process of PCP into the cells.
D000818:Animals; D001665:Binding Sites; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells; D007700:Kinetics; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism*; D010622:Phencyclidine / Q000378:metabolism / Q000494:pharmacology*
D010622:Phencyclidine