[The fluorescent staining of mitochondria in living HeLa- and LM-cells with new acridine dyes (author's transl)].
The fluorescent staining of mitochondria in living cells with new acridine dyes is reported. The fluorescent dyes used are derivatives of acridine orange (AO) and of 3-amino-6-methoxyacridine (AMA) with various residues in 9- or 10-position (Scheme 1). They are either permanent cationic dyes or cations which are formed by protonation in the culture medium. HeLa cells and mouse fibroblasts (LM cells) have been used for our staining experiments. On favourable conditions we succeeded in staining the mitochondria not only orthochromatically but also metachromatically. Photodynamical effects which have been observed during the exposure of the stained cells in the fluorescence microscope are described. The residues in 9- or 10-position favour the dye accumulation in the mitochondria. Vital staining with the basic compounds AO and AMA however leads to the formation of metachromatically stained lysosomes in the orthochromatically stained cytoplasm. The dye 3-amino-6-methoxy-9-(2-hydroxyethyl)acridine stains the nucleus of living cells.
D000165:Acridine Orange; D000609:Aminoacridines; D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000648:ultrastructure; D005456:Fluorescent Dyes; D006367:HeLa Cells / Q000648:ultrastructure; D006801:Humans; D051379:Mice; D008928:Mitochondria / Q000648:ultrastructure*
D000609:Aminoacridines; D005456:Fluorescent Dyes; D000165:Acridine Orange
Uber die Vitalfluorochromierung von Mitochondrien in HeLa- und LM-Zellen mit neuen Acridinfarbstoffen.