Evidence for the vivo compartmentation of amino acids between blood cells and plasma in man with liver disease during constant infusion of L (U-14C) tyrosine.
L (U-14C) tyrosine tracer was infused at a constant rate for 8 hrs in a series of five patients with liver disease on two occasions, the first when diet consisted of intravenous glucose, and the second when aminoacids were added. Plateau labelling of both plasma and intracellular blood cell free tyrosine was obtained by 6 hr of each infusion. However, the intracellular specific activities were on average 50% lower (p less than 0.05) when diet was glucose alone, and 54% lower (p less than 0.01) when glucose and aminoacids were given. Change in diet did not significantly affect these differences. The results provide evidence for significant in-vivo compartmentation of aminoacids between plasma and blood cells in man with liver disease, and indicate that whole blood cannot be used in the conventional measurement of whole body protein.
D000596:Amino Acids / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D008107:Liver Diseases / Q000097:blood*; D008875:Middle Aged; D010949:Plasma / Q000378:metabolism; D014443:Tyrosine / Q000097:blood*
D000596:Amino Acids; D014443:Tyrosine