5-methyl-dCTP deaminase induced by bacteriophage XP-12.
Bacteriophage XP-12, whose DNA contains 34 mol% 5-methylcytosine, induces the synthesis of a unique enzyme, 5-methyl-dCTP deaminase. The substrate for this enzyme, 5-methyl-dCTP, is produced by reactions catalyzed in part by other phage-induced enzymes, and the product of the reaction is dTTP. The deaminase therefore provides a novel pathway for biosynthesis of thymine residues for phage XP-12 DNA. Evidence is presented that this pathway is used for dTTP synthesis in XP-12-infected Xanthomonas oryzae.
D001435:Bacteriophages / Q000201:enzymology*; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D003641:Deamination; D003845:Deoxycytosine Nucleotides / Q000378:metabolism; D004790:Enzyme Induction; D009709:Nucleotide Deaminases / Q000096:biosynthesis*; D011741:Pyrimidine Nucleosides / Q000378:metabolism; D011742:Pyrimidine Nucleotides / Q000378:metabolism; D013936:Thymidine / Q000494:pharmacology; D013942:Thymine Nucleotides / Q000096:biosynthesis; D014974:Xanthomonas / Q000378:metabolism*
D004269:DNA, Bacterial; D004279:DNA, Viral; D003845:Deoxycytosine Nucleotides; D011741:Pyrimidine Nucleosides; D011742:Pyrimidine Nucleotides; D013942:Thymine Nucleotides; C034817:5-methyldeoxycytidine triphosphate; C034825:5-methyl-dCTP deaminase; D009709:Nucleotide Deaminases; C024157:thymidine 5'-triphosphate; D013936:Thymidine