Effect of monoclonal anti-neuraminidase antibodies on the kinetic behavior of influenza virus neuraminidase.
Neuraminidase from the recombinant influenza virus A/NWSHA-Tokyo/3/67NA HON2 has been shown to exhibit non-Michaelis-Menten kinetics. The multiphasic behaviour was demonstrated for both the isolated neuraminidase heads and for the intact virus. Interaction of the enzyme with two monoclonal anti-neuraminidase antibodies (WANA 1 and RANA 1), which recognize separate antigenic determinants on the molecule, resulted in hyperbolic kinetic behaviour. While both antibodies abolished the multiphasic kinetics of the enzymic reaction, only WANA 1 altered the Vmax and Km values, indicating that it may in some way inhibit the interaction of enzyme and substrate.
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D009980:Influenza A virus / Q000201:enzymology*; D007700:Kinetics; D009439:Neuraminidase / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D009439:Neuraminidase