A method of dark-field and simultaneous light--dark-field illumination for photomacrography of autoradiographic preparations.
A technique is described for dark-field and light--dark-field illumination of autoradiographic preparations for photomacrogra. A right-angle prism is employed as a substrate through which illumination is introduced to the specimen. The technique permits uniform illumination of large fields of view, and also minimizes the image-degrading effects of chromatic dispersion and excessive noncoherent scatter which are often associated with conventional dark-field illumination.
D000818:Animals; D001345:Autoradiography / Q000295:instrumentation / Q000379:methods*; D001921:Brain / Q000033:anatomy & histology*; D002415:Cats; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods*; D010784:Photomicrography / Q000295:instrumentation / Q000379:methods*