Isolation and partial characterization of a toxin from the venom of the East African orthognath spider Pterinochilus spec.
A minor basic polypeptide in the venom of the East African orthognath spider Pterinochilus spec. was the most toxic of 16 basic components in this venom. This toxin A4/4 was purified by gel permeation on Sephadex G-50 and ion exchange chromatography on CM-Sephadex C-25 with a yield of 1.3% of the total protein content of the crude venom. The polypeptide has an isoelectric point of 9.39 and molecular weight of 10,500. The s.c. LD50 in mice is 0.1 mg/kg. Death occurred due to respiratory paralysis. After administration of sublethal doses rapid reversibility of the symptoms and total recovery of the animals was observed.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001180:Arthropod Venoms / Q000032:analysis*; D002850:Chromatography, Gel; D007525:Isoelectric Focusing; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D013111:Spider Venoms / Q000032:analysis*; D014118:Toxins, Biological / Q000032:analysis* / Q000633:toxicity
D000596:Amino Acids; D001180:Arthropod Venoms; D010455:Peptides; D013111:Spider Venoms; D014118:Toxins, Biological