An ELISA procedure for detecting human anti-endotoxin antibodies in serum.
We report an ELISA method suitable for the large-scale screening of blood bank stores to identify those blood units containing high concentrations of antiendotoxin antibodies. In Natal, 8.3% of total units collected had antiendotoxin antibodies at concentrations greater than 40 micrograms/ml, values that may be therapeutically useful. We found that one technician could screen enough samples per year to produce 800 litres of such high-titre plasma.
D000907:Antibodies, Bacterial / Q000032:analysis*; D004731:Endotoxins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D008403:Mass Screening
D000907:Antibodies, Bacterial; D004731:Endotoxins