[Abortive influenza virus infection of Ehrlich cells. Virion and subcellular fraction proteins].
Ehrlich ascitic carcinoma cells infected with the WSN strain of influenza virus produced noninfectious virus particles containing no M protein. Polypeptides P1, NP, and NS were synthesized in the infected ascites cells to the same concentration was in permissive cells (chick fibroblasts and MDCK cells); the synthesis of HA and M proteins was markedly disturbed, however. In the permissive cells, M protein was found in the fraction of the perinuclear martial and plasma membranes whereas in the ascitic cells M protein was detected only in perinuclear material but not in plasma membrane. Thus, nonpermissiveness of the ascitic cells appears to be due to disturbance of both synthesis and transport of M protein to the plasma membrane.
D000818:Animals; D001345:Autoradiography; D002286:Carcinoma, Ehrlich Tumor / Q000382:microbiology*; D002642:Chick Embryo; D003673:Defective Viruses / Q000032:analysis / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D051379:Mice; D009842:Oligopeptides / Q000032:analysis; D009975:Orthomyxoviridae / Q000032:analysis* / Q000378:metabolism; D009976:Orthomyxoviridae Infections / Q000382:microbiology*; D013347:Subcellular Fractions / Q000382:microbiology; D014764:Viral Proteins / Q000032:analysis* / Q000096:biosynthesis; D014771:Virion / Q000032:analysis* / Q000378:metabolism; D014776:Virus Cultivation
D009842:Oligopeptides; D014764:Viral Proteins
Abortivnaia infektsiia virusa grippa v kletkakh Erlikha. Belki virionov i subkletochnykh fraktsiĭ.