Phospholipase activities of rat brain cytosol. Occurrence of phospholipase C activity with phosphatidylcholine.
21-day-old rat brain contains a soluble phospholipase C with the ability to hydrolyse phosphatidylcholine. This enzyme has an alkaline pH optima. The results of the DEAE-cellulose fractionation, the pH profile and the Ca2+-dependency suggest that the enzyme may be the same as that responsible for phosphatidylinositol hydrolysis. The activity of the phospholipase C is associated closely with a diacylglycerol lipase. The two enzyme activities could be separated by DEAE-cellulose fractionation, resulting in greater than 100% apparent recovery of the phospholipase C acivity. This phospholipase C activity is not the result of the back reaction of choline phosphotransferase.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002118:Calcium / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D008071:Lipoprotein Lipase / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D051381:Rats; D010738:Type C Phospholipases / Q000378:metabolism*
D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D010740:Phospholipases; D008071:Lipoprotein Lipase; D010738:Type C Phospholipases; D002118:Calcium