Immunoassay for the steroid-induced isoenzyme of alkaline phosphatase in the dog.
Antiserum directed against the steroid-induced alkaline phosphatase (ALP) isoenzyme of the dog was produced in rabbits. This antiserum was used to develop a sensitive and quantitative assay for the detection of the steroid-induced ALP isoenzyme in the serum of dogs. This antiserum was also used to enhance the electrophoretic separation of the steroid-induced and hepatic ALP isoenzymes.
D000305:Adrenal Cortex Hormones / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D003480:Cushing Syndrome / Q000201:enzymology / Q000662:veterinary*; D004283:Dog Diseases / Q000201:enzymology*; D004285:Dogs; D007106:Immune Sera; D007118:Immunoassay / Q000662:veterinary; D007527:Isoenzymes / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008297:Male; D011817:Rabbits / Q000276:immunology
D000305:Adrenal Cortex Hormones; D007106:Immune Sera; D007527:Isoenzymes; D000469:Alkaline Phosphatase