Effect of epileptogenic agents on the incorporation of 3H-glycine into proteins in the cat's cerebral cortex.
Filter paper strips soaked in 3H-glycine solution were applied to acoustic cortex of cats, anaesthetized with Nembutal and pretreated with epileptogenic agents (Metrazol, G-penicillin, and 3-amino-pyridine) and cycloheximide. The untreated contralateral hemisphere served as control. After 1 h incubation, both cortical samples were excised simultaneously and fixed in Bouin solution for autoradiography. Incorporation was blocked by cycloheximide. There was no glycine incorporation on the penicillin-treated side, while pyramidal cells were intensively labelled in layers II-V of the mirror focus. 3-Aminopyridine produced the same result. Metrazol as convulsant proved to be far weaker than the previous two. The intensity of incorporation was significantly more intensive in the mirror focus than in the primary one. Penicillin and 3-aminopyridine, while provoking cortical seizures, seem to inhibit glycine incorporation into a neuron-specific, function-dependent protein contained by the labelled cells in the autoradiogram.
D000161:Acoustic Stimulation; D000631:Aminopyridines / Q000494:pharmacology; D000818:Animals; D002415:Cats; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D003292:Convulsants / Q000494:pharmacology*; D003513:Cycloheximide / Q000494:pharmacology; D004827:Epilepsy / Q000378:metabolism*; D005998:Glycine / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism* / Q000494:pharmacology; D010400:Penicillin G / Q000494:pharmacology; D010433:Pentylenetetrazole / Q000494:pharmacology; D014316:Tritium
D000631:Aminopyridines; D003292:Convulsants; D009419:Nerve Tissue Proteins; D014316:Tritium; D003513:Cycloheximide; D010400:Penicillin G; D005998:Glycine; D010433:Pentylenetetrazole