[Use of a countercurrent immunoelectrophoretic method for isolating antistaphylococcal antibodies and staphylococcal antigen in the blood serum of children with staphylococcal infections].
In cases of staphylococcal infection in children (osteomyelitis, purulent destructive pneumonia, phlegmons, abscesses) the use of counter immunoelectrophoresis (CIE) allowed one to detect antistaphylococcal antibodies (anti-poly-A beta) in 12.5-69.1% of cases and staphylococcal antigen in the blood serum in 6.1-25.0% of cases, the antigen titer reaching 1 : 1-1 :8. In staphylococcal sepsis with faintly pronounced local pyo-inflammatory processes, as well as in diseases of nonstaphylococcal etiology and in healthy children, no clear reactions indicating the presence of antistaphylococcal antibodies and staphylococcal antigen were registered. CIE may be used as a specific serological diagnostic test in the severe forms of staphylococcal infection in children.
D000293:Adolescent; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D003378:Counterimmunoelectrophoresis / Q000379:methods*; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D007223:Infant; D007231:Infant, Newborn; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*; D013682:Teichoic Acids / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D013682:Teichoic Acids
Primenenie metoda vstrechnogo immunoélektroforeza s tsel'iu vyiavleniia protivostafilokokkovykh antitel i stafilokokkovogo antigena v syvorotke krovi deteĭ so stafilokokkovymi infektsiiami.