Ex vivo shunt testing of hydrogel-silicone rubber composite materials.
Hydrogel polymers consisting of HEMA (2-hydroxyethyl methacrylate) alone or co-polymerized with NVP N-(vinyl pyrrolidone) were incorporated into silicone rubber tubing to produce hydrophilic composite materials. Polymerization methods of the interpenetrating network (IPN) and radiation-induced type were used to impregnate the silicone rubber substrates. These methods produced materials with varied wettability (contact angle with water ranged from 57 degrees to 95 degrees) but similar texture. The surface morphology as observed in the scanning electron microscope was the same for all the materials. Four types of composite materials and two sizes of silicone rubber control tubing in a total of 19 dogs were used as ex vivo femoral artery-to-vein (A-V) shunts and were sampled at 5, 15, 30, 45, and 60 min. Data obtained by using scanning electron microscopy (SEM) were used to determine the type and amount of adhering blood cells at the time intervals described. Large platelet aggregates and/or a variable initial response were found to be predictors of shunt failure (occlusion). Most samples showed only varied levels of platelet adhesion, but other cell types were seen in some samples. The adhesion was found to peak in all cases by 30 min and to reach lower levels by 60 min, suggesting that an initial phase of reaction was complete. The course of platelet adhesion for a 60-min period is presented.
D000818:Animals; D001166:Arteriovenous Shunt, Surgical / Q000295:instrumentation; D001672:Biocompatible Materials; D001773:Blood Cells / Q000502:physiology*; D004285:Dogs; D005069:Evaluation Studies as Topic; D008297:Male; D008689:Methacrylates; D008855:Microscopy, Electron, Scanning; D010973:Platelet Adhesiveness; D010976:Platelet Count; D019736:Prostheses and Implants; D011760:Pyrrolidinones; D012826:Silicone Elastomers; D013997:Time Factors
D001672:Biocompatible Materials; D008689:Methacrylates; D011760:Pyrrolidinones; D012826:Silicone Elastomers; C005044:hydroxyethyl methacrylate