Simple purification method for anti-glycolipid antibody using polystyrene latex beads coated with gangliotetraosylceramide.
A simple method for the purification of antiglycolipid antibody is described. Polystyrene latex beads coated with gangliotetraosylceramide (GA1) were used as immunoadsorbent for the purification of anti-GA1 antibody. The antibody bound to the GA1-coated latex beads was released with 1 M NaSCN solution. The glycolipid-coated latex beads seem valuable not only for the purification of antibodies but also as a tool for investigating the function of glycolipids.
D000906:Antibodies / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D005732:Gangliosides; D006017:Glycolipids / Q000276:immunology*; D006028:Glycosphingolipids; D007840:Latex; D011137:Polystyrenes
D000906:Antibodies; C011258:G(A1) ganglioside; D005732:Gangliosides; D006017:Glycolipids; D006028:Glycosphingolipids; D007840:Latex; D011137:Polystyrenes