An electron microscopic demonstration of immune complexes of hepatitis B e-antigen using colloid gold as a marker.
Immune complexes of the hepatitis B e-antigen (HBeAg) could be labeled and thus visually identified in the electron microscope by using antibody to HBeAg (anti-HBe) tagged with colloidal gold particles. Circulating immune complexes of HBeAg were detected in sera from patients with acute hepatitis B infections as well as from asymptomatic carriers of hepatitis B surface antigens (HBsAg). Sera positive for rheumatoid factor frequently contained mixed aggregates in which immune complexes of HBsAg were closely bound to immune complexes of HBeAg.
D000914:Antibodies, Viral / Q000276:immunology*; D000936:Antigen-Antibody Complex; D002712:Chlorides; D003102:Colloids; D006046:Gold; D017612:Gold Compounds; D006509:Hepatitis B / Q000276:immunology*; D006510:Hepatitis B Antibodies / Q000276:immunology*; D006511:Hepatitis B Antigens / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D006513:Hepatitis B e Antigens / Q000276:immunology*; D006801:Humans; D008854:Microscopy, Electron; D012217:Rheumatoid Factor / Q000276:immunology
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D002712:Chlorides; D003102:Colloids; D017612:Gold Compounds; D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D006046:Gold; C024568:gold tetrachloride, acid; D012217:Rheumatoid Factor