Evidence of the mannosylation of a non-histone protein in monkey liver chromatin.
Mannose is incorporated in monkey liver chromatin by the means of a nuclear membrane mannosyltransferase. 14C-labelled chromatin is dissociated either by sulfuric acid or 6 M urea and 0.4 M GuCl. The fractions then enriched in non-histone 14C-labelled proteins are excluded from Ultro-gel AcA 202, their analysis in SDS-polyacrylamide gel electrophoresis shows that radioactivity fits with one major protein band, confirming the presence of at least a non-histone protein labelled with mannose in monkey liver chromatin, with an apparent molecular weight of 13,000.
D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D006602:Hexosyltransferases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008362:Mannosides / Q000378:metabolism; D008364:Mannosyltransferases / Q000378:metabolism*; D009685:Nuclear Envelope / Q000201:enzymology
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D008362:Mannosides; D006602:Hexosyltransferases; D008364:Mannosyltransferases