A simple high-performance liquid chromatographic pre-column technique for investigation of drug metabolism in biological fluids.
A simple reversed-phase high-performance liquid chromatographic pre-column technique for investigation of drug metabolism is described. This rapid method circumvents the "classical" sample purification via extraction by direct purification and enrichment of the sample on the pre-column. Almost 100% recovery of a drug (aminopyrine) and its metabolites from biological fluids is achieved. This is in strong contrast to "classical" sample preparation which allows a recovery of 30-100% depending on the polarity of the investigated compound. The procedure described has been successfully applied to the investigation of the metabolic pattern of aminopyrine in rat plasma and cell incubation media.
D000632:Aminopyrine / Q000097:blood* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003470:Culture Media / Q000032:analysis; D051381:Rats; D012016:Reference Values
D003470:Culture Media; D000632:Aminopyrine