A modified equilibrium dialysis method for studying fatty acid binding to proteins.
A modified equilibrium dialysis method is described which is suitable for investigating the binding of fatty acids in the form of aqueous micellar dispersions to proteins. The method uses a permeant chromophore which complexes reversibly with free fatty acid within the dialysis bag. The concentration outside the dialysis bag is determined spectrophotometrically. Binding of oleic acid to bovine serum albumin is given as an example. A simplified analysis of fatty acid binding is given and used to indicate the potential of the method.
D000818:Animals; D002417:Cattle; D003956:Dialysis / Q000379:methods; D005227:Fatty Acids / Q000378:metabolism*; D008823:Micelles; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D011796:Quinacrine / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013053:Spectrophotometry; D013997:Time Factors
D005227:Fatty Acids; D008823:Micelles; D009829:Oleic Acids; D011506:Proteins; D012710:Serum Albumin, Bovine; D019301:Oleic Acid; D011796:Quinacrine