Fractionation of apolipoproteins from human serum very low density lipoproteins by chromatofocusing.
1. A pooled serum from several pregnant women was used as a source of VLDL 2. VLDL and if needed other lipoproteins were fractionated by sequential flotation. 3. Lipoproteins were delipidated and lipid-free VLDL apolipoproteins were fractionated by a new chromatofocusing technique. 4. Chromatofocusing column run yielded 7 peak protein fractions and the corresponding pI values were: 6.8, 6.6, 5.7, 5.5, 5.2, 4.8 and 4.4. 5. Polyacrylamide slab gel electrophoresis of the chromatofocusing protein peaks indicated that they are different having dissimilar Rf values in urea-SDS containing slabs.
D001053:Apolipoproteins / Q000097:blood* / Q000302:isolation & purification; D002845:Chromatography / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D008079:Lipoproteins, VLDL / Q000097:blood* / Q000302:isolation & purification; D011247:Pregnancy
D001053:Apolipoproteins; D008079:Lipoproteins, VLDL