Monocyte interactions with solid substrates monitored by chemiluminescence.
The kinetics of interaction of solid substrates with mononuclear cells is a little explored by important feature in the evaluation of potential prosthetic materials. Monocyte production of chemiluminescence in a luminol-enhanced system was used to explore such kinetics. A theoretical model of cell/surface interaction is developed and alternatives in the evaluation of data generated by chemiluminescent curves are presented. The interactions of monocytes with a polytetrafluoroethylene (PTFE) surface were similar to those seen in studies on phagocytosis and were dependent on an absorbed serum protein layer. This study illustrates the use of a simple laboratory test for monocyte oxidative function in assessing the potential inflammatory effects of various prosthetic materials.
D001672:Biocompatible Materials; D005069:Evaluation Studies as Topic; D006801:Humans; D007249:Inflammation / Q000276:immunology; D008163:Luminescent Measurements; D009000:Monocytes / Q000276:immunology*; D009895:Opsonin Proteins / Q000276:immunology*; D010587:Phagocytosis; D011138:Polytetrafluoroethylene; D013997:Time Factors
D001672:Biocompatible Materials; D009895:Opsonin Proteins; D011138:Polytetrafluoroethylene