The Thy-1 glycoprotein on nerve cells in culture: electron-microscopic analysis and biochemical characterization.
The distribution and some biochemical properties of the Thy-1 glycoprotein, an antigen common to brain and thymus-derived lymphocytes, were analyzed in primary nerve cell cultures from mouse embryos. The Thy-1 antigen was detected on the plasma membrane of most neurons and fibroblast-like cells and on some astrocytes. The labelling pattern was homogeneous, except on some neuronal cell bodies where the distribution varied from a few patches to a homogeneous labelling. The Thy-l molecules immunoprecipitated from either nerve cell cultures or from fibroblast-like cells from the meninges had similar molecular weights and isoelectric points. In contrast, Thy-1 from thymocytes was more heterogeneous in molecular weight and in isoelectric point range.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000032:analysis / Q000648:ultrastructure; D005727:Ganglia, Spinal / Q000032:analysis / Q000648:ultrastructure; D006023:Glycoproteins / Q000032:analysis*; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000032:analysis* / Q000648:ultrastructure; D013116:Spinal Cord / Q000032:analysis / Q000648:ultrastructure
D000954:Antigens, Surface; D006023:Glycoproteins