Isolation of a retrovirus from cultured equine sarcoid tumor cells.
A virus with the morphologic and biochemical properties of the family Retroviridae has been isolated from cultured cells explanted from a malignant tumor induced by intradermal inoculation of equine sarcoid cells into a combined immunodeficient Arabian foal. By electron microscopy, intracytoplasmic, extracellular, and budding particles measuring 89 to 120 nm with electron-lucent cores were seen. Virus purified from the medium of cultured cells had a buoyant density of 1.15 g/cm3 in isopycnic sucrose-gradient centrifugation, incorporated radiolabeled uridine but not thymidine, and had constitutive RNA-dependent DNA polymerase which required Mn2+ for optimal endogenous activity.
D000818:Animals; D002478:Cells, Cultured; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D008274:Magnesium / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D008854:Microscopy, Electron; D012190:Retroviridae / Q000302:isolation & purification* / Q000378:metabolism / Q000648:ultrastructure; D012878:Skin Neoplasms / Q000382:microbiology / Q000662:veterinary*; D014529:Uridine / Q000378:metabolism
D008345:Manganese; D008274:Magnesium; D014529:Uridine