Comparison of ciliostasis by mycoplasmas in mouse and chicken tracheal organ cultures.
The cilia-stopping effect of mycoplasmas of human and various animal origin in mouse and chicken tracheal organ cultures was studied. From the results in mouse tracheal organ cultures, the mycoplasma strains tested were divided into three groups: Mycoplasma pulmonis m53, M pulmonis JB, M. pulmonis OK, M. mycoides subsp. Mycoides PGl and M. Gallisepticum S6 showed a strong cilia-stopping effect; M. pulmonis PG22, M. mycoides subsp. capri PG3, M. meleagridis 19729, M. neurolyticum Type A and M. arthritidis PG6 showed a mild effect; and M. pneumoniae FH, M. salivarium Hup, M. hominis type 1-C and M. orale N-C human origin and Acholeplasma laidlawii PG8 showed a weak effect. On the other hand, in chicken tracheal organ cultures, only M. gallisepticum S6 showed a strong effect, M. meleagridis 19729 was affected to a lesser degree, and the other mycoplasma strains showed a weak or no effect. The results indicate that some murine and poultry mycoplasmas showed a cilia-stopping tendency in mouse and chicken tracheal organ cultures, respectively, while human mycoplasmas showed weak or little effect in both organ cultures. In mouse tracheal organ cultures, M. pulmonis m53 treated with heat, trypsin or formaldehyde, and the sterile filtrate of an m53 broth culture showed no cilia-stopping effect. The relationship of the pathogenicity of mycoplasmas for their natural hosts to that for cultured respiratory cells is discussed.
D000818:Animals; D002645:Chickens / Q000502:physiology; D002923:Cilia / Q000502:physiology*; D006801:Humans; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009068:Movement; D009174:Mycoplasma / Q000145:classification / Q000502:physiology*; D009924:Organ Culture Techniques; D014132:Trachea / Q000502:physiology* / Q000648:ultrastructure