In vitro packaging of bacteriophage T7 DNA requires ATP.
Removal of nucleoside triphosphates from extracts prepared from bacteriophage T7-infected Escherichia coli results in a stringent requirement for added ATP to form infective phage particles by in vitro packaging of bacteriophage T7 DNA. Optimal packaging efficiency was achieved at a concentration of about 1.25 mM. Other nucleoside triphosphates could be substituted for ATP, but none of the common nucleoside triphosphates was as effective as ATP in promoting in vitro encapsulation.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D004279:DNA, Viral / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D009711:Nucleotides / Q000494:pharmacology; D013604:T-Phages / Q000378:metabolism*
D004279:DNA, Viral; D009711:Nucleotides; D000255:Adenosine Triphosphate