A rapid isolation technique for drugs from tissues and fluids: use of the Du Pont Prep 1 system.
A microprocessor-controlled, automated extraction/concentration device, the Prep 1 (Du Pont Clinical Systems), was evaluated for application to the isolation of drug substances from postmortem fluids and tissue homogenates. Two classes of materials were investigated: barbiturates and the benzodiazepine, diazepam. With as little as 200 mg of tissue, barbiturate derivatives were successfully isolated and measured by gas chromatography using nitrogen-phosphorus detection with a coefficient of variation of 2 to 5%. Diazepam was measured in a similar fashion with a coefficient of variation of 4.4%. Preliminary investigation indicates that this system is applicable to a wide range of drug substances of toxicological interest.
D001344:Autopsy / Q000379:methods; D001463:Barbiturates / Q000032:analysis; D001826:Body Fluids / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods*; D003975:Diazepam / Q000032:analysis; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D004364:Pharmaceutical Preparations / Q000032:analysis*; D014116:Toxicology / Q000379:methods*
D001463:Barbiturates; D004364:Pharmaceutical Preparations; D003975:Diazepam