Fertilization of hamster ova by human spermatozoa in relation to other semen parameters.
Different indices of the zona-free hamster ovum test system were interrelated. High correlations were found between the fertilization percentage and the average number of penetrated spermatozoa/ovum (r = 0.99) and between the fertilization percentage and the number of spermatozoa attached to the ova surfaces (r = 0.72). Fertilization percentages from 63 subfertile patients were correlated with different semen factors assessed by multiple exposure photography (MEP). Low correlations were found between sperm concentration and fertilization percentage (r = 0.29) and between fertilization percentage and motile sperm count (r = 0.33). Spermatozoa progressing with high average velocity had low fertilization rates compared to specimen with moderate average velocity. Effects of sperm washing on the in vitro fertilizing capacity were studied in a group of 40 subfertile men. The fertilization percentages of 8 specimens--with visual seminal plasma abnormalities--increased significantly when immediate dilution of semen was applied.
D000818:Animals; D001681:Biological Assay / Q000379:methods; D006224:Cricetinae; D005260:Female; D005306:Fertilization; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000503:physiopathology; D010781:Photography; D012661:Semen / Q000502:physiology*; D013076:Sperm Count; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology