The combined effects of oxygen tension, X radiation and 5-thio-D-glucose on the survival of mammalian cells.
The glucose analogue 5-thio-D-glucose (5-TG) is toxic to hypoxic cells but does not affect aerated cells. Toxicity is markedly dependent upon oxygen tension: exposure of Chinese hamster cells to 5 mM 5-TG and 50 ppm O2 (in the overlying atmosphere) results in a survival of 0.01 in six hours, whereas increasing the oxygen to 100 ppm causes the survival to increase to about 0.9. The combined effect of X rays and 5-TG is to reduce the survival of hypoxic cells to a level far below that attainable with either agent alone. Cells made hypoxic with 5 ppm O2 have 0.1 survival after three hours exposure to 5 mM 5-TG. The same cells have a 0.5 survival when given a dose of 1000 rad. When the x-irradiation is given at the end of a three-hour exposure to 5-TG, the survival is reduced to 0.0055.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects*; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D005947:Glucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D008168:Lung; D010100:Oxygen / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D013997:Time Factors; D014965:X-Rays
D011838:Radiation-Sensitizing Agents; C003002:5-thio-D-glucose; D005947:Glucose; D010100:Oxygen