The phosphorylation of proteins in hippocampal slices: effects of noradrenaline and of pretreatment with kainic acid.
Hippocampal slices were incubated in the presence of [32P]P1, and protein phosphorylation was examined by means of sodium dodecyl sulfate-gel electrophoresis. Incubation for at least 30 min with 300 muCi of [32P)P1/brain slice gave rise to the phosphorylation of 8-10 protein bands. Most of these bands showed enhanced phosphorylation in response to noradrenaline. The basal phosphorylation of kainic acid-pretreated hippocampal slices was enhanced two- to threefold compared with controls. There was also an additional increase in kainic acid-pretreatment slices in the response to noradrenaline. 8-Br-Cyclic AMP and phosphodiesterase inhibitors, such as papaverine or isobutylmethylxanthine, had no effect on the phosphorylation patterns.
D000818:Animals; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007608:Kainic Acid / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology*; D010750:Phosphoproteins / Q000378:metabolism*; D010761:Phosphorus Radioisotopes / Q000378:metabolism; D010766:Phosphorylation; D011433:Propranolol / Q000494:pharmacology; D011759:Pyrrolidines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; D011759:Pyrrolidines; D011433:Propranolol; D007608:Kainic Acid; D009638:Norepinephrine