Determination of DNA superhelicity and extremely low levels of DNA strand breaks in low numbers of nonradiolabeled cells by DNA-4',6-diamidino-2-phenylindole fluorescence in nucleoid gradients.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000032:analysis*; D004278:DNA, Superhelical / Q000032:analysis*; D005260:Female; D005347:Fibroblasts / Q000032:analysis; D005456:Fluorescent Dyes; D006801:Humans; D007211:Indoles; D008099:Liver / Q000378:metabolism; D009690:Nucleic Acid Conformation; D051381:Rats; D013050:Spectrometry, Fluorescence
D004278:DNA, Superhelical; D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI; D004247:DNA