Genetic analysis of an N-ethyl-N-nitrosourea-induced mutation at the hemoglobin beta-chain locus in mice.
The specific-locus test was performed with N-ethyl-N-nitrosourea (ENU) on spermatogonia of mice by using visible loci and a hemoglobin beta-chain (Hbb) locus. Male wild-type (C3Hf/He) mice were injected intraperitoneally with ENU. 8 weeks after injection, these males were mated with tester strain females (PW). 9 visible mutants and the 1 Hbb locus mutant were obtained. This Hbb locus mutation was heritable and viable under homozygous conditions, suggesting that it might have resulted from events at the molecular level.
D000818:Animals; D003433:Crosses, Genetic; D004588:Electrophoresis, Cellulose Acetate; D005038:Ethylnitrosourea / Q000494:pharmacology*; D005260:Female; D006454:Hemoglobins / Q000235:genetics*; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009154:Mutation; D009607:Nitrosourea Compounds / Q000494:pharmacology*
D006454:Hemoglobins; D046911:Macromolecular Substances; D009607:Nitrosourea Compounds; D005038:Ethylnitrosourea