In vitro effects of sex steroids on LH-stimulated steroid accumulation by isolated rabbit ovarian follicles.
The in vitro effects of progesterone, testosterone and estradiol-17 beta on steroid accumulation by isolated rabbit follicles were examined. Progesterone had no effect on LH-stimulated androgen accumulation but inhibited LH-stimulated estrogen accumulation at 10(-7) M and 10(-6) M. Testosterone at 10(-5) M but not at 10(-6) M or 10(-7) M, inhibited LH-stimulated progesterone accumulation. LH-stimulated estrogen accumulation was inhibited at all dose levels of testosterone. Estradiol (10(-5) M) inhibited LH-stimulated androgen accumulation and had no effects on progesterone accumulation. It is concluded that the steroidal milieu of follicles can influence their response to LH.
D000818:Animals; D004958:Estradiol / Q000378:metabolism; D005260:Female; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000494:pharmacology*; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism; D011817:Rabbits; D013739:Testosterone / Q000378:metabolism
D012739:Gonadal Steroid Hormones; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone