A simple method for electrophoretic analysis of cell surface glycoproteins based on concanavalin A binding.
A simple and sensitive method is described for the detection of cell surface proteins. This method consists of reacting concanavalin A (Con A) with 35S-labeled methionine, detergent solubilized whole cell extracts and immunoprecipitating the Con A-protein complexes with anti-Con A antibody bound to Staphylococcus aureus Cowan I strain (SACI). These reagents are commercially available, stable and easy to standardize. Mouse lymphocytes cell surface proteins purified by this procedure show great similarities to those purified with goat anti-cell surface antibodies.
D000818:Animals; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D011952:Receptors, Concanavalin A
D006023:Glycoproteins; D008565:Membrane Proteins; D011952:Receptors, Concanavalin A