Selection in HAT medium is not a reliable method for the study of reversion from 6-thioguanine resistance to sensitivity.
6-Thioguanine-resistant mutants were induced in V79 Chinese hamster cells with 2-aminopurine, ICR-170 and hycanthone. Samples of mutants of different origin were treated with EMS or 1CR-170 and plated in HAT medium for selection of revertants. In the result, a significant fraction of HAT-resistant clones was not 6-thioguanine-sensitive as would have been expected from a genuine reversion to wild type.
D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D004351:Drug Resistance; D008168:Lung; D013866:Thioguanine / Q000494:pharmacology*
D003470:Culture Media; D013866:Thioguanine