Fluorescent age pigment in insect lysosomes.
Fluorescent pigment accumulates in the lysosomal fraction of spodoptera sixth instars. It was purified by silicic acid chromatography. The fluorescent fraction was characterized by conjugated unsaturation, reactivity with thiobarbituric acid, and lability in alkali; all reminiscent of age pigment. The phosphorus content of the purified fraction was negligible and nitrogen was not detected. It is suggested that the material is degraded, perhaps polymerized lipid fragments.
D000375:Aging; D000818:Animals; D007814:Larva / Q000254:growth & development; D007915:Lepidoptera / Q000254:growth & development* / Q000378:metabolism; D008062:Lipofuscin / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism*; D010860:Pigments, Biological / Q000302:isolation & purification / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013858:Thiobarbiturates
D008062:Lipofuscin; D010860:Pigments, Biological; D013858:Thiobarbiturates; C029684:thiobarbituric acid