Identification of some bacteriophage T4 prereplicative proteins on two-dimensional gel proteins.
Profiles of bacteriophage T4 early proteins resolved by a two-dimensional nonequilibrium pH gradient electrophoresis system (P. Z. O'Farrell, H. M. Goodman, and P. H. O'Farrell, Cell 12:1133--1142, 1977) are presented. Over 65 phage-induced proteins were resolved. Amber or deletion mutants were used to identify 17 proteins in the gel patterns as the products of specific genes.
D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D013604:T-Phages / Q000032:analysis* / Q000254:growth & development; D014764:Viral Proteins / Q000302:isolation & purification*; D014779:Virus Replication
D014764:Viral Proteins