[Pectolytic enzymes formed by Penicillium and Fusarium micromycetes].
The ability of the two cultures Penicillium sp. and Fusarium sp. to synthesize extracellular pectolytic enzymes was investigated. The cultivation conditions providing a high level of the biosynthesis of these enzymes were identified. The methods of isolating the enzymes by means of organic solvents were developed. The pectinase from Penicillium sp. showed a higher thermostability whereas that from Fusarium sp. displayed a greater acid resistance. Using glutaraldehyde and titanium salts, active immobilized forms of pectinases on silica carriers were prepared.
D004355:Drug Stability; D004800:Enzymes, Immobilized / Q000378:metabolism; D005670:Fusarium / Q000201:enzymology*; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D006358:Hot Temperature; D010407:Penicillium / Q000201:enzymology*; D011096:Polygalacturonase / Q000096:biosynthesis* / Q000302:isolation & purification
D004800:Enzymes, Immobilized; D006026:Glycoside Hydrolases; D011096:Polygalacturonase
Pektoliticheskie fermenty, obrazuemye, mikrometsetami Penicillium i Fusarium.