Effect of anti-HCG sera on purified syncytiotrophoblast from full-term human placenta.
Heterologous anti-HCG and anti-beta-HCG sera failed to effect complement-dependent lysis of purified 51chromium-labeled syncytiotrophoblast in vitro. The progesterone synthetic activity of syncytiotrophoblast was also preserved in the presence of the anti-HCG sera. Binding of the anti-HCG antibodies to the syncytiotrophoblast was demonstrated in an immunofluorescence test. The potential of the anti-HCG sera to participate in complement dependent lysis of other HCG-bearing cell types was revealed using BeWo cells as the targets; 53%-68% of the latter were killed by exposure to anti-HCG sera and complement. The possible mechanism(s) whereby syncytiotrophoblast resists damage by the anti-HCG sera are considered.
D000906:Antibodies; D006063:Chorionic Gonadotropin / Q000037:antagonists & inhibitors / Q000276:immunology*; D003165:Complement System Proteins; D003267:Contraception; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D011247:Pregnancy; D011374:Progesterone / Q000096:biosynthesis; D014327:Trophoblasts / Q000276:immunology* / Q000378:metabolism
D000906:Antibodies; D006063:Chorionic Gonadotropin; D011374:Progesterone; D003165:Complement System Proteins