Purification and properties of a mitogenic lectin from Lathyrus sativus seeds.
A mitogenic lectin has been isolated from saline extract of Lathyrus sativus by (NH4)2SO4 precipitation, chromatography on DEAE-Sepharose and subsequent affinity chromatography on Sephadex G-100. The lectin has a molecular weight of 49,000, as determined by ultracentrifugation, and consists of heavy (Mr 19,000) and light subunits (Mr about 4,400). The amino acid composition and N-terminal sequence of both subunits are given. The lectin agglutinated human erythrocytes of different ABO groups equally well, and the agglutination is inhibited best by D-mannose and D-glucose and their alpha-methyl-glucosides. High concentrations of the lectin were needed for optimal stimulation of human lymphocytes.
D000596:Amino Acids / Q000032:analysis; D037102:Lectins / Q000302:isolation & purification*; D008934:Mitogens / Q000302:isolation & purification*; D008970:Molecular Weight; D010835:Phytohemagglutinins / Q000302:isolation & purification; D037121:Plant Lectins; D010944:Plants
D000596:Amino Acids; D037102:Lectins; D008934:Mitogens; D010835:Phytohemagglutinins; D037121:Plant Lectins