The effect of uranyl acetate on human lymphoblastoid cells (RPMI 6410) and HeLa cells.
RPMI 6410 cells and HeLa cells were exposed to uranyl acetate. In RPMI 6410 cell cultures this produced single-membrane-bound presumably lysosomal bodies (called "uraniosomes") containing electron-dense crystals in the cultured cells and crystalline deposits in extracellular locations. Neither uraniosomes nor extracellular uranium deposits were found in HeLa cell cultures. All uraniosomes and extracellular uranium deposits analysed by electron-probed X-ray analysis were found to contain uranium, potassium and phosphorus. Traces of sulphur were detected in some but not all uraniosomes and extracellular uranium deposits. Traces of calcium were found in all extracellular uranium deposits and in some uraniosomes also.
D002118:Calcium / Q000032:analysis; D002478:Cells, Cultured; D004577:Electron Probe Microanalysis; D006367:HeLa Cells / Q000032:analysis* / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D008214:Lymphocytes / Q000032:analysis* / Q000187:drug effects / Q000648:ultrastructure; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D008933:Mitochondrial Swelling / Q000187:drug effects; D009942:Organometallic Compounds; D010758:Phosphorus / Q000032:analysis; D011188:Potassium / Q000032:analysis; D014501:Uranium / Q000032:analysis / Q000494:pharmacology*
D009942:Organometallic Compounds; D010758:Phosphorus; C005460:uranyl acetate; D014501:Uranium; D011188:Potassium; D002118:Calcium