Leptospiral vaccines in dogs: immunogenicity of whole cell and outer envelope vaccines prepared in protein-free medium.
The immunogenicity of leptospires cultivated in modified bovine albumin-polysorbate 80 medium and those cultivated in protein-free medium were quantitatively evaluated in the dog. Vaccine preparations, whole cell or outer envelope, prevented leptospiremia; however, kidney culture data revealed that 1 of 4 dogs vaccinated with 0.1 to 1 mg of whole cell prepared from leptospires cultivated in the modified bovine albumin-polysorbate 80 medium was positive for Leptospira, whereas dogs vaccinated with whole cells prepared in protein-free medium were not. Dogs vaccinated with greater than or equal to 0.5 mg of outer envelope were refractory to infection after challenge exposure.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000917:Antibody Formation; D001428:Bacterial Vaccines / Q000276:immunology*; D003470:Culture Media; D004283:Dog Diseases / Q000382:microbiology / Q000517:prevention & control*; D004285:Dogs; D007668:Kidney / Q000382:microbiology; D007921:Leptospira interrogans / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D007922:Leptospirosis / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D003470:Culture Media