New method for hemoglobin determination by using sodium lauryl sulfate (SLS).
We have developed a new method for hemoglobin determinations using sodium lauryl sulfate (SLS), a surfactant. The detailed action of SLS is still unknown, but presumably SLS will convert hemoglobin into methemoglobin in the order of oxyhemoglobin, hemochrome and methemoglobin, and its oxidative activity. Therefore, unlike others, this method does not need oxidative reagents and does not generate toxic wastes such as KCN and NaN3 which cause environmental pollution. The proposed method is applicable for the manual and Hemalog-8/90 procedures often used in clinical laboratories. Tentatively we have designated it as the "SLS-methemoglobin (SLS-Hb) method."
D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D012967:Sodium Dodecyl Sulfate; D013054:Spectrophotometry, Atomic / Q000379:methods
D006454:Hemoglobins; D012967:Sodium Dodecyl Sulfate