Determination of immunoglobulin subclasses by electrosyneresis.
The author applied the method or electrosyneresis to distinguish the subclasses of monoclonal IgG and IgA immunoglobulins. In agarose gel with high electroendosmosis the antibody-active fraction of immunosera migrates toward the cathode while the immunoglobulin molecules bound to albumin by glutaraldehyde toward the anode. In the meeting zone of specific immunoserum and immunoglobulin--albumin complex a precipitation arch develops if immunochemical identity exists. This method is extremely sensitive, economic and rapid. It is very suitable for the distinction of the subclasses of monoclonal immunoglobulins.
D003378:Counterimmunoelectrophoresis / Q000379:methods*; D007122:Immunoelectrophoresis / Q000379:methods*; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007136:Immunoglobulins / Q000145:classification*
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007136:Immunoglobulins