Multiple signals in STEM.
A brief description is given of the eight image signals that can be simultaneously recorded in digital form using an appropriate data recording system and high resolution STEM; this involves a set of detectors covering bright and dark field. Details are provided of the collection and manipulation of image data for STEM, including on-line estimation of off-focus distance, on-line assessment of astigmatism, on-line signal to noise ratio in any or all detectors; examples of all three procedures are given. The use of the data recording system is discussed together with some aspects of image display, including the use of colour. A method is introduced of providing, using the appropriate hardware, a prescribed colour map (image intensity converted to colour) using replaceable PROMS. Two off-line procedures are considered. One is the Karhunen-Loeve transform and its application to bringing together the information contained within the multiple signals, and the other is the discrete cosine transform by which the storage problem for digital image storage may be reduced, according to the image, by between 15 and 30 times.
D000818:Animals; D003201:Computers; D003421:Crithidia / Q000648:ultrastructure*; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000379:methods*