6-deoxy-D-glucose and D-xylose: analogs for the study of D-glucose transport by mouse 3T3 cells.
6-Deoxy-D-glucose and D-xylose, structural homomorphs of D-glucose that lack a 6-hydroxyl group or a 6-hydroxymethyl group, respectively, are transported efficiently by mouse 3T3 cells, with good affinity and high specificity for the D-glucose transport system. Since these analogs lack the 6-hydroxyl group, which is the site of phosphorylation of glucose by hexokinase, they are taken up and are recoverable from cells in an unchanged state. Thus, 6-deoxy-D-glucose and D-xylose offer advantages as transport substrates over 2-deoxy-D-glucose, which is phosphorylated by intercellular hexokinases, and 3-O-methyl-D-glucose, which shows a lower specificity for the D-glucose transport system.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000378:metabolism*; D003837:Deoxy Sugars; D003847:Deoxyglucose; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D013329:Structure-Activity Relationship; D014994:Xylose
D003837:Deoxy Sugars; D003847:Deoxyglucose; D014994:Xylose; D005947:Glucose