Localization of hydrogenase in Thiocapsa roseopersicina photosynthetic membrane.
The photosynthetic cell membrane is impermeable to the oxidized redox dyes Methyl Viologen and Benzyl Viologen, whereas the reduced forms easily penetrate into the cells. By exploiting this permeability difference, the orientation of the membrane-bound hydrogenase has been determined.
D001594:Benzyl Viologen / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology; D002463:Cell Membrane Permeability; D002841:Chromatiaceae / Q000201:enzymology*; D006859:Hydrogen / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D010269:Paraquat / Q000378:metabolism; D010788:Photosynthesis; D013104:Spheroplasts / Q000201:enzymology
D001594:Benzyl Viologen; D006859:Hydrogen; D010088:Oxidoreductases; D010269:Paraquat