Separation of intermediates in the refolding of reduced erabutoxin b by analytical isoelectric focusing in layers of polyacrylamide gel.
Isoelectric focusing in a thin layer of polyacrylamide gel is shown to be a suitable method for the resolution of intermediates trapped during the refolding process of reduced cystine-containing proteins. The method has been applied to the well-characterized snake neurotoxin erabutoxin b. An explanation is offered for the relatively low rate of refolding of this polypeptide.
D000818:Animals; D003720:Densitometry; D004546:Elapid Venoms / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004870:Erabutoxins / Q000378:metabolism*; D007525:Isoelectric Focusing; D007700:Kinetics; D009498:Neurotoxins / Q000378:metabolism*; D010084:Oxidation-Reduction; D011487:Protein Conformation; D011489:Protein Denaturation
D004546:Elapid Venoms; D009498:Neurotoxins; D004870:Erabutoxins; C032917:erabutoxin b