Comparison of various techniques for determining viability of Paracoccidioides brasiliensis yeast-form cells.
The viability of Paracoccidioides brasiliensis yeast-form cells was determined by colony-forming units, direct fluorescent staining, and production of germ tubes in slide culture. The first procedure was unreliable and time consuming; the latter two showed better correlation with hemacytometer total cell counts and required significantly less time.
D002470:Cell Survival; D003470:Culture Media; D005658:Fungi / Q000254:growth & development*; D006801:Humans; D010228:Paracoccidioides / Q000254:growth & development* / Q000302:isolation & purification; D010229:Paracoccidioidomycosis / Q000209:etiology
D003470:Culture Media