[Percentage of monocytes in preparations of mononuclear cells from normal subjects: evaluation by a new rapid method for the identification of esterase-positive cells].
A rapid suspension method for alpha-naphthyl acetate esterase staining has been utilized to evaluate monocytes percentages in Ficoll-Paque separated mononuclear cells (MC) from normal individuals. In MC preparations from 24 normal subjects a mean percentage of 8% monocytes has been found, with a range from 2% to 20%. Cell fractions depleted of T-lymphocytes by rosette sedimentation showed 43% of esterase positive cells (range 31-53%), while very few monocytes (2%) appeared to contaminate the preparations of E-rosetting cells. The method for esterase staining of monocytes while they are in suspension is very simple and permits a rapid and accurate evaluation of monocyte percentages in mixed cell preparations.
D000328:Adult; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D002469:Cell Separation / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology* / Q000201:enzymology; D009283:Naphthol AS D Esterase / Q000097:blood*; D012016:Reference Values; D013997:Time Factors
D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases
Percentuali di monociti in preparazioni di cellule mononucleari di soggetti normali: valutazione mediante un nuovo metodo rapido per la identificazione delle cellule esterasi positive.