Characterization of myosin heavy chain by cyanogen bromide peptide maps.
Procedures have been developed for the preparation of pure myosin heavy chain (h-myosin) by preparative gel electrophoresis, and for the characterization of h-myosin by cyanogen bromide peptide mapping. Major sources of error are the oxidation of methionine and the proteolytic splitting of the chain during purification. These errors have been eliminated. A peculiar feature is the doubling or quadrupling of a peptide of molecular weight 17 000. The results show structural differences between isomyosins derived from myonal types within the same animal, as well as interspecies differences.
D000818:Animals; D003488:Cyanogen Bromide; D046911:Macromolecular Substances; D008970:Molecular Weight; D009218:Myosins / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis; D011817:Rabbits
D046911:Macromolecular Substances; D010446:Peptide Fragments; D009218:Myosins; D003488:Cyanogen Bromide