Some characteristics of beta-naphthyl oligophosphates as allosteric effectors of human hemoglobin.
1. Fluorescent organic phosphates. beta-naphthyl oligophosphates, were found to interact with human hemoglobin, and to lower the oxygen affinity. The magnitude of the allosteric effect depended on the number of phosphoric groups in each compound. 2. In analogy with inositol hexaphosphate (IHP). beta-naphthyl tetraphosphate (beta-NapP4) was found to interact with oxyhemoglobin. Therefore, the spectral changes in oxyhemoglobin and carboxyhemoglobin induced by beta-NapP4 and IHP were compared. 3. The extent of spectral changes in oxyhemoglobin induced by beta-NapP4 was smaller than that induced by IHP, whereas the extent of spectral changes in carboxyhemoglobin induced by beta-NapP4 was about twice that by IHP. 4. These results indicate that there is a structural difference between oxyhemoglobin and carboxyhemoglobin.
D000494:Allosteric Regulation / Q000187:drug effects; D002263:Carboxyhemoglobin / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D006801:Humans; D009284:Naphthols / Q000097:blood / Q000494:pharmacology*; D009943:Organophosphorus Compounds / Q000097:blood / Q000494:pharmacology*; D010108:Oxyhemoglobins / Q000378:metabolism*; D010833:Phytic Acid / Q000494:pharmacology; D011485:Protein Binding; D013053:Spectrophotometry; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
D009284:Naphthols; D009943:Organophosphorus Compounds; D010108:Oxyhemoglobins; D010833:Phytic Acid; D002263:Carboxyhemoglobin