Conditions for the generation of high serum levels of C3DP.
High titers of C3DP, a DNA-binding derivative of complement C3, have been reported to occur in the sera of individuals with active malignant disease. The mechanism of generation of C3DP remained obscure. We report here that C3DP is not generated in vitro under the conditions required for its isolation and subsequent assay. However, under certain storage conditions, all sera (normal and malignant) produce C3DP. This severely limits the usefulness of the assay for diagnostic and prognostic purposes.
D001769:Blood / Q000378:metabolism; D001793:Blood Preservation; D002352:Carrier Proteins / Q000032:analysis / Q000096:biosynthesis*; D002846:Chromatography, Affinity; D004268:DNA-Binding Proteins; D005189:False Positive Reactions; D006801:Humans; D009363:Neoplasm Proteins; D009369:Neoplasms / Q000097:blood*; D011863:Radioimmunoassay
C013867:C3DP protein, human; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins