A column chromatographic method for the removal from 2-amino-2-methyl-1-propanol of impurities that inhibit alkaline phosphatase activity.
The selective removal from 2-amino-2-methyl-1-propanol solutions of impurities that inhibit alkaline phosphatase activity by means of Amberlite chromatography is described. The effects of buffer concentration, column dimensions, adsorbent type, and zinc sulphate addition were investigated. The method was found to improve greatly the quality of commercial 2-amino-2-methyl-1-propanol solutions and to have several advantages over alternative purification methods.
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D004340:Drug Contamination / Q000517:prevention & control; D011412:Propanolamines / Q000302:isolation & purification*
D011412:Propanolamines; D000469:Alkaline Phosphatase; C006551:2-amino-2-methyl-1-propanol