Multiplexed time-lapse photomicrography of cultured cells.
A system of cinemicrography has been developed in which a single microscope and 16 mm camera are multiplexed to produce a time-lapse photographic record of many fields simultaneously. The field coordinates and focus are selected via a control console and entered into the memory of a dedicated microcomputer; they are then automatically recalled in sequence, thus permitting the photographing of additional fields in the interval between exposures of any given field. Sequential exposures of each field are isolated in separate sections of the film by means of a specially designed random-access camera that is also controlled by the microcomputer. The need to unscramble frames is thereby avoided, and the developed film can be directly analysed.
D000818:Animals; D002478:Cells, Cultured / Q000502:physiology / Q000648:ultrastructure*; D008838:Microcomputers; D008853:Microscopy / Q000295:instrumentation; D010784:Photomicrography / Q000379:methods*