Enzyme histochemical changes in murine osteoarthritis.
Male mice of the STR/ORT strain spontaneously develop an osteoarthritic lesion in the medial tibial condyles. Histochemical studies of lactate dehydrogenase have previously shown that a region of completely inactive chondrocytes develops in the cartilage prior to degenerative changes. The present studies have shown a nearly parallel time course for the development of inactive cells and cartilage damage, except that there is a delay of four to five weeks between these two events. It is considered that the latter is a consequence of the former. The mechanism of the enzyme depletion is not known, but it probably extends to many, if not all, oxidative enzymes, and it is suggested that a disturbance in a hydrogen transport (cytochrome) system could account for the apparent lack of activity of many dehydrogenases as demonstrated by tetrazolium salt techniques.
D000818:Animals; D002356:Cartilage / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006651:Histocytochemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D051379:Mice; D008954:Models, Biological; D009243:NAD / Q000378:metabolism; D010003:Osteoarthritis / Q000209:etiology / Q000378:metabolism*; D010084:Oxidation-Reduction
D006025:Glycosaminoglycans; C020341:glucosaminoglycans; D009243:NAD; D007770:L-Lactate Dehydrogenase