Identification and characterization of excretory-secretory products of Brugia malayi, adult filarial parasites.
Although E-S antigens may be particularly important for both the pathogenesis and immunodiagnosis of helminth infections, little is known about the immunochemistry or functional roles in human filarial infections. In the present paper, we have done some initial identification and characterization of E-S products of adult Brugia malayi by employing a combination of sensitive biochemical and immunochemical techniques. E-S products, collected by incubating B. malayi adults in vitro in a defined protein-free medium, were radiolabeled with 125I. SDS-polyacrylamide gel electrophoresis (PAGE) and autoradiography of labeled E-S products revealed 11 protein bands in the m.w. range of 10,000 to 70,000. Comparison of radiolabeled E-S products and adult somatic antigen (B.m.A) in SDS-PAGE indicated many common bands, and crossed immunoelectrophoresis and competitive Staph-A RIA confirmed the presence of most E-S antigens in B.m.A. Of the 11 E-S bands, two appeared to be derived from the surface of the adult worms and microfilariae as shown by SDS-PAGE and autoradiography of lodogen surface-labeled parasites; the presence of two host proteins in E-S was detected by crossed-line immunoelectrophoresis. The E-S antigens were highly immunogenic when tested both with rabbit antiserum raised against B.m.A and with a serum pool of patients with natural filarial infection.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000941:Antigens / Q000032:analysis* / Q000276:immunology; D001345:Autoradiography; D001667:Binding, Competitive; D002009:Brugia / Q000276:immunology / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005368:Filariasis / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology; D005849:Gerbillinae; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008297:Male; D011817:Rabbits; D011863:Radioimmunoassay
D000906:Antibodies; D000941:Antigens