Adsorption of human alpha (leukocyte) interferon on glass: contributions of electrostatic and hydrophobic forces.
Human leukocyte-derived interferon (HuIFN-alpha) can be displaced from controlled-pore glass (CPG) with alkylamines at 0.1-0.3 M concentration. The eluting power of an alkylamine correlates positively with the extent of its alkylation: CH3NH3 + less than (CH3)2NH2 + less than (CH3)3NH+ less than (CH3)4N+. Interferon can also be recovered using an electrolyte and ethylene glycol as cosolvents. Apparently, both hydrophobic and electrostatic forces are involved in the adsorption of HuIFN-alpha to CPG.
D000327:Adsorption; D000478:Alkylation; D000588:Amines; D004560:Electricity; D005898:Glass; D006801:Humans; D007370:Interferon Type I / Q000302:isolation & purification; D012997:Solvents
D000588:Amines; D007370:Interferon Type I; D012997:Solvents