[Isolation of phospholipase A2 on biospecific supports].
The procedure of isolation of phospholipase A2 from Agkistrodon halys blomhoffii venom and porcine pancreas on biospecific supports of an organo-silica type with immobilized phospholipid is described. The purity of isolated enzymes was controlled by polyacrylamide gel electrophoresis, determination of the N-terminal amino acid and isoelectrofocusing. The enzyme activity was equal to 80 mumole/mg/min and 1400 mumole/mg/min for snake venom phospholipase and for pancreatic phospholipase A2, respectively.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D003435:Crotalid Venoms; D007700:Kinetics; D010179:Pancreas / Q000201:enzymology*; D010740:Phospholipases / Q000302:isolation & purification*; D010741:Phospholipases A / Q000302:isolation & purification* / Q000378:metabolism; D054467:Phospholipases A2; D013552:Swine
D000596:Amino Acids; D003435:Crotalid Venoms; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2
Vydelenie fosfolipazy A2 na biospetsificheskikh sorbentakh.