Monoclonal antibodies recognize different parts of Z-DNA.
Brominated poly(dG-dC) . poly(dG-dC), which forms a stable Z-DNA helix under physiological salt conditions, was used as an antigen to produce monoclonal antibodies from mice. The anti-Z-DNA antibodies were specific for the Z conformation as was shown by direct binding studies and a competitive solid phase assay. Competition could only be seen by brominated poly(dG-dC) . poly(dG-dC) under low and high salt conditions and unmodified poly(dG-dC) . poly(dG-dC) under high salt conditions. No binding could be seen to a number of other polynucleotides, including native and denatured DNA. Among the anti-Z-DNA monoclonal antibodies, examples differed in specificity: binding of one monoclonal antibody was blocked by methylation of cytosine; whereas the other antibody reacted equally well with the unmodified polymer or methyl cytosine-containing polymer in high ionic strength. The two antibodies are specific for different localized features of Z-DNA structure.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D001665:Binding Sites; D001966:Bromine; D004247:DNA / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008810:Mice, Inbred C57BL; D008958:Models, Molecular; D011089:Polydeoxyribonucleotides / Q000276:immunology
D000911:Antibodies, Monoclonal; D011089:Polydeoxyribonucleotides; C019530:poly(dC-dG); D004247:DNA; D001966:Bromine