Ribosomal DNA of Leishmania brasiliensis: number of ribosomal copies and gene isolation.
The DNA content of the hemoflagellate Leishmania brasiliensis, strain Y, has been determined by colorimetric reactions and found to be nearly 0.226 pg/cell. When this DNA is bound to filters and hybridized with labeled rRNA from the same organism, saturation is reached at 0.47% of the DNA, corresponding to an estimated 160 ribosomal gene copies. When the DNA is sheared and centrifuged to equilibrium in CsCl gradients, two major satellites of the main band (rho = 1.712 g/cm3) are observed: a heavy one (1.720 g/cm3), which hybridizes with labeled rRNA, and a light one (1.699 g/cm3) with the electron microscopic characteristics of the kinetoplast DNA network.
D000818:Animals; D004247:DNA / Q000032:analysis / Q000302:isolation & purification*; D004275:DNA, Ribosomal; D005796:Genes; D007891:Leishmania / Q000032:analysis / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012335:RNA, Ribosomal / Q000032:analysis / Q000235:genetics*
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D004247:DNA