Quantitative characteristics of the stabilizing effect of glycerol on cytochrome P-450 in rabbit liver microsomes.
Kinetics of thermal inactivation of the rabbit liver microsomal cytochrome P-450 has been studied in the temperature range of 45-54 degrees C with increasing concentrations of glycerol. For all temperatures studied in the absence and presence of glycerol, thermal inactivation of cytochrome P-450 is characterized by two phases, the first being described by the first order rate constants. Glycerol markedly lowers the rate of thermal inactivation of cytochrome P-450 as well as the activation parameters delta H and delta S of the process of thermal destruction. There is a linear relationship between decreasing values of delta H, delta S and increasing glycerol concentrations in the medium.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005990:Glycerol / Q000494:pharmacology*; D006358:Hot Temperature; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D011817:Rabbits; D013816:Thermodynamics
D003577:Cytochrome P-450 Enzyme System; D005990:Glycerol