Characterization of glutathione S-transferase activity in Echinococcus granulosus.
1. Glutathione S-transferase activity has been characterized in protoscolices of Echinococcus granulosus. 2. The conjugation reaction was activated by treatment of protoscolices with phenobarbital and beta-naphthoflavone. 3. Protoscolices of E. granulosus contained the conjugation cofactor glutathione in the amount of 287 nmol/g fresh tissue. 4. Conjugation may represent an important factor in the resistance of the parasite to drugs.
D000818:Animals; D001571:Benzoflavones / Q000494:pharmacology; D004137:Dinitrochlorobenzene / Q000378:metabolism; D004446:Echinococcus / Q000201:enzymology*; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010634:Phenobarbital / Q000494:pharmacology; D013053:Spectrophotometry; D013347:Subcellular Fractions / Q000201:enzymology; D013696:Temperature; D019324:beta-Naphthoflavone
D001571:Benzoflavones; D004137:Dinitrochlorobenzene; D019324:beta-Naphthoflavone; D005982:Glutathione Transferase; D005978:Glutathione; D010634:Phenobarbital