Insensitivity of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity to low-density lipoprotein in concanavalin-A-resistant Chinese-hamster ovary cells.
A concanavalin-A-resistant mutant of Chinese-hamster ovary cells has been shown to have altered cholesterol content and 3-hydroxy-3-methylglutaryl coenzyme A reductase activity compared to wild-type and spontaneous-revertant cell lines. These changes are associated with insensitivity of the mutant reductase activity to suppression by low-density lipoprotein and impaired receptor-mediated binding and uptake of 125I-labelled low-density lipoprotein.
D000818:Animals; D002460:Cell Line; D002738:Chloroquine / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology*; D006224:Cricetinae; D003412:Cricetulus; D004351:Drug Resistance; D005260:Female; D006888:Hydroxycholesterols / Q000494:pharmacology; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000378:metabolism / Q000494:pharmacology*; D010053:Ovary
D006888:Hydroxycholesterols; D008077:Lipoproteins, LDL; D003208:Concanavalin A; C007997:25-hydroxycholesterol; D002738:Chloroquine; D006903:Hydroxymethylglutaryl CoA Reductases