Interaction of acidic liposomes with red blood cells. Induction of endocytosis and shedding of particles.
Calcium ions induced tight binding of massive amounts of liposomes containing cardiolipin, phosphatidyl-ethanolamine and phosphatidylcholine to erythrocytes. Initially, liposome-liposome fusion occurred and only subsequently the resulting large structures adhered to cells. Large clumps composed of liposomes and cells were formed. Upon prolonged incubation, the clumps were dissipated spontaneously and excess liposomes were released. A constant amount of phospholipid (15-25 nmol/10(8) cells) were incorporated into the cell membranes. Upon disaggregation, the cells shed erythrocyte particles. The latter were isolated and shown to contain lipids from both cellular and liposomal origin. The particles lacked spectrins and contained variable amounts of band 3 content. Liposomes induced endocytosis in reticulocytes but not in mature erythrocytes. In most cases, the liposomes themselves were not engulfed by the cells and remained attached to their surface. The relevance of this phenomenon to delivery to liposome contents into cells is discussed.
D000327:Adsorption; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002308:Cardiolipins / Q000378:metabolism; D004705:Endocytosis / Q000187:drug effects; D004912:Erythrocytes / Q000502:physiology*; D008081:Liposomes / Q000378:metabolism* / Q000494:pharmacology; D008854:Microscopy, Electron; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000502:physiology
D002308:Cardiolipins; D008081:Liposomes; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D002118:Calcium