Analysis of fat-soluble vitamins. XXVII. High performance liquid chromatographic and gas-liquid chromatographic determination of vitamin D in fortified milk and milk powder: collaborative study.
A collaborative study of the high performance liquid chromatographic (HPLC) method for vitamin D in fortified milkpowder (skimmed and whole milk) and a milkpowder preparation was carried out on 182 samples distributed to 26 laboratories. Thirteen laboratories submitted results. The level of vitamin D was 2-7 IU vitamin D3/g milkpowder. All samples also contained vitamin A. Three laboratories were excluded from the statistical evaluation because of incomplete results or deviation from the analytical procedure. Other laboratories reported acceptable results. At the same time, 63 samples were distributed to 9 laboratories which used a gas-liquid chromatographic method for determining vitamin D in milkpowder. Only one laboratory reported results. The HPLC method has been adopted official first action.
D000818:Animals; D002417:Cattle; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D005223:Fats; D005519:Food Preservation; D005527:Food, Fortified / Q000032:analysis; D008892:Milk / Q000032:analysis*; D012995:Solubility; D014807:Vitamin D / Q000032:analysis*
D005223:Fats; D014807:Vitamin D