DNA repair ability of cultured cells derived from mouse embryos in comparison with human cells.
DNA repair in mouse cells derived from embryos of 3 inbred strains were investigated in comparison with that in human cells. The levels of unscheduled DNA synthesis after UV irradiation appeared to change at different passages, but capacities of host-cell reactivation of UV-irradiated herpes simplex virus were always reduced to the same levels as those in xeroderma pigmentosum cells. This implied that mouse cells are reduced in excision-repair capacities and that the apparently high levels of unscheduled DNA synthesis at certain passages are not quantitatively related to high levels of cell survival. Essentially no differences in DNA repair were noted among 3 strains--BALB/c, C3H/He and C57BL/10.
D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004260:DNA Repair; D004261:DNA Replication; D004622:Embryo, Mammalian / Q000502:physiology* / Q000528:radiation effects; D005260:Female; D005865:Gestational Age; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy; D013045:Species Specificity; D014466:Ultraviolet Rays