Radioimmunoassay of measles virus hemagglutinin protein G.
Guinea pig and rabbit antisera from animals immunized with purified measles virus hemagglutinin (G) protein were used to establish a solid-phase four-layer radioimmunoassay for quantitative measurement of the G protein. The sensitive of the assay was 2 ng of purified G protein, and 200 micrograms of protein from uninfected Vero cells neither decreased the sensitivity nor reacted non-specifically in the assay. Radioimmunoassay standard dose-response curves were established and unknown values interpolated from these using the logit program of a desktop computer. Using this procedure, a measles virus growth curve in infected Vero cells was determined by measurement of G protein production. Under these same conditions, hemagglutination was not sensitive enough to detect early hemagglutinin production. Viral antigens in canine distemper virus, Newcastle disease virus, parainfluenza viruses 1-4, simian virus 5, and respiratory syncytial virus-infected cell lysates did not cross-react in the radioimmunoassay. A small degree of cross-reactivity was detected with mumps viral antigens, both with Vero cell-derived (wild-type strain) and egg-derived (Enders strain) purified virus preparations and with a cell lysate antigen prepared from wild-type mumps virus-infected Vero cells.
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003429:Cross Reactions; D006386:Hemagglutination Tests; D006389:Hemagglutinins, Viral / Q000032:analysis*; D008459:Measles virus / Q000254:growth & development / Q000276:immunology*; D009109:Mumps virus / Q000276:immunology; D011863:Radioimmunoassay; D013045:Species Specificity
D006389:Hemagglutinins, Viral