Effect of ethanol consumption on the phospholipid composition of rat liver microsomes and mitochondria.
Male Sprague-Dawley rats were maintained for 31 days on a liquid diet containing 36% of calories as ethanol. Pair-fed controls were administered a similar diet, but with maltose-dextrin isocalorically substituted for ethanol. A phospholipid analysis has been carried out in liver microsomes and mitochondria isolated from the two groups of animals. The phospholipid phosphorus/protein ratio was not significantly different in the organelles of the ethanol-fed animals as compared to the same organelles of liquid diet controls, which indicates that ethanol feeding did not influence the total phospholipid content of microsomes and mitochondria. The phospholipid distribution within organelles was not changed, except for a significant increase in the phosphatidylinositol content of microsomes from ethanol-fed animals. The fatty acid compositions of both microsomal and mitochondrial phospholipids were significantly altered by ethanol feeding. In microsomes from ethanol-fed rats, palmitic acid levels were lowered in the total phospholipid fraction, phosphatidylcholine and phosphatidylethanolamine; oleic acid levels were elevated in microsomal phosphatidylethanolamine. In mitochondria from ethanol-fed animals, palmitic and arachidonic acid were lowered in phosphatidylcholine and phosphatidylethanolamine. Oleic and linoleic acid were elevated in the same phospholipids. In contrast, linoleic acid levels in cardiolipin were depressed significantly. These alterations in the fatty acid composition are suggestive of ethanol-induced changes in fatty acid desaturation activities.
D000818:Animals; D002308:Cardiolipins / Q000032:analysis; D000431:Ethanol / Q000494:pharmacology*; D008297:Male; D008862:Microsomes, Liver / Q000032:analysis / Q000187:drug effects*; D008930:Mitochondria, Liver / Q000032:analysis / Q000187:drug effects*; D009929:Organ Size / Q000187:drug effects; D010743:Phospholipids / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D002308:Cardiolipins; D010743:Phospholipids; D000431:Ethanol