The toxic A-chain of mistletoe lectin I: isolation and its effect on cell-free protein synthesis.
The reduction of Sepharose bound mistletoe lectin I (ML I) is a convenient method for preparing A- and B-chains under mild conditions. The B-chain represents the D-galactose binding moiety. The A-chain shows a significant dose-dependent inhibition of protein synthesis in cell-free systems from rat liver and rabbit reticulocytes, while pure B-chain preparations do not affect ribosomal translation processes.
D000818:Animals; D002474:Cell-Free System; D037102:Lectins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D014784:Mistletoe; D008970:Molecular Weight; D037121:Plant Lectins; D010946:Plants, Medicinal; D011132:Polyribosomes / Q000187:drug effects; D014176:Protein Biosynthesis; D011817:Rabbits; D051381:Rats; D012156:Reticulocytes / Q000187:drug effects
D037102:Lectins; D037121:Plant Lectins