Autoradiographic demonstration of binding sites for [3H]desipramine in rat brain.
Many of the characteristics of [3H]desipramine ([3H]DMI) binding sites, previously established with brain homogenates, were confirmed in slide-mounted sections of rat forebrain. A 1:1 ratio of specific to non-specific binding was established. The proportion of specifically bound [3H]DMI was reduced in brain sections from rats pretreated with 6-hydroxydopamine but not with 5,6-dihydroxytryptamine. High resolution localization of [3H]DMI binding sites was achieved by exposing the sections to tritium-sensitive film.
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins; D003891:Desipramine / Q000378:metabolism*; D005260:Female; D007099:Imipramine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011955:Receptors, Drug / Q000378:metabolism*
D002352:Carrier Proteins; D011955:Receptors, Drug; C036079:desipramine receptor; D007099:Imipramine; D003891:Desipramine