Effect of inhibitors of cholesterol synthesis on muscle differentiation.
Aggregation, the first step in the fusion of cultured chick myoblasts to form myotubes, is inhibited by 90% after only 2 h exposure to 25-hydroxycholesterol, an inhibitor of cholesterol synthesis. In this study, the effect of this agent on the concentration of an integral membrane protein in the plasma membrane, the synthesis of two differentiation-controlled proteins and total protein synthesis were examined. While it is possible that any of these mechanisms could be responsible for the inhibition of fusion, none of them appear to account for the attenuation of fusion induced by the inhibition of cholesterol synthesis.
D000818:Animals; D002454:Cell Differentiation; D002642:Chick Embryo; D002784:Cholesterol / Q000096:biosynthesis*; D006888:Hydroxycholesterols / Q000494:pharmacology; D009132:Muscles / Q000166:cytology* / Q000187:drug effects; D013997:Time Factors
D006888:Hydroxycholesterols; C007997:25-hydroxycholesterol; D002784:Cholesterol