Gas chromatographic determination of primary and secondary amines as pentafluorobenzamide derivatives.
An analytical method is described for the determination of mono- and dialkylamines in foodstuffs. Amines are derivatized to their pentafluorobenzamides which may be separated by gas chromatography (GC) and determined using an N-P detector. Analysis of the derivatives by GC-mass spectrometry indicated they were all mono-substituted. The amines were isolated from foodstuffs by alkaline distillation of greater than or equal to 75% sample volume. The distribution of dimethylamine (DMA) in distillate volumes indicated 2 maxima from barley and malt, which could represent 2 or more sources of DMA. DMA concentrations of 6.6-8.8 ppm in barley, 11 ppm in malt, and 1.2 ppm in beer are higher than previously reported.
D000588:Amines / Q000032:analysis*; D001565:Benzoates; D002849:Chromatography, Gas; D004123:Dimethylamines / Q000032:analysis; D002523:Edible Grain / Q000032:analysis*; D001467:Hordeum / Q000032:analysis*; D013058:Mass Spectrometry; D005464:Fluorobenzenes; D005466:Fluorocarbons
D000588:Amines; D001565:Benzoates; D004123:Dimethylamines; C014737:pentafluorobenzoyl chloride; D005464:Fluorobenzenes; D005466:Fluorocarbons