Design and synthesis of new reactive fluorescent dyes for cytofluorometry.
Our purpose has been to synthesize reactive fluorescent compounds with different excitation and/or emission spectra that can be used for multiple fluorescence analysis in combination with the "natural" cell fluorescence exhibited by reduced coenzyme (NAD(P)H) or flavins. Synthesis of condensation products of p-bis(2-chloroethyl)-amino-benzaldehyde is described. Their absorption spectra range from 360 to 480 nm and their emission spectra lie between 520 and 590 mm. Examples of cytological applications are given.
D055598:Chemical Phenomena; D002621:Chemistry; D003067:Coenzymes / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D007700:Kinetics; D009588:Nitrogen Mustard Compounds; D009698:Nucleoproteins / Q000032:analysis
D003067:Coenzymes; D005456:Fluorescent Dyes; D009588:Nitrogen Mustard Compounds; D009698:Nucleoproteins