Inhibition of phospholipase A2 activity by Fluosol, an artificial blood substitute.
This paper describes the effect of Fluosol on phospholipase A2 activity both in vitro and in vivo. This compound inhibited both postheparin plasma and hepatic phospholipase A2 activities. Furthermore, in Fluosol transfused rats the postheparin plasma phospholipase A2 activity was 41% below that observed in control animals. The inhibition of phospholipase A2 activity was due to the micellar forms of pluoronic, F-68, a detergent present in Fluosol. It is postulated that the inhibition of phospholipase A2 activity by Fluosol might interfere with the release of arachidonic acid, a substrate used in biosynthesis of prostaglandins.
D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology*; D003840:Deoxycholic Acid / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology*; D005466:Fluorocarbons / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology; D008297:Male; D017830:Octoxynol; D010740:Phospholipases / Q000037:antagonists & inhibitors*; D010741:Phospholipases A / Q000037:antagonists & inhibitors* / Q000097:blood; D054467:Phospholipases A2; D011060:Poloxalene / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000494:pharmacology*; D013997:Time Factors
D001802:Blood Substitutes; D003902:Detergents; D005466:Fluorocarbons; D013501:Surface-Active Agents; D003840:Deoxycholic Acid; C008807:perfluorotributylamine; D011092:Polyethylene Glycols; D017830:Octoxynol; D011060:Poloxalene; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2