Amino-acid sequence of the vitamin-K-dependent part of protein Z.
After tryptic digestion of protein Z a peptide corresponding to residues 1-43 has been isolated and sequenced. Smaller peptides were generated by treatment of the peptide with pepsin and then separated by high-performance liquid chromatography. The purity of the peptides was sufficient for mass spectrometric measurement. Thirteen gamma-carboxyglutamic acid residues have been identified and the peptide shows strong homology to the corresponding domains of the classical vitamin-K-dependent blood clotting proteins.
D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000302:isolation & purification; D014812:Vitamin K / Q000494:pharmacology*
D001798:Blood Proteins; D010446:Peptide Fragments; C026080:plasma protein Z; D014812:Vitamin K