Sequence specificity in DNA for the interaction with adriamycin or daunomycin.
The interactions of adriamycin and daunomycin with various polydeoxyribonucleotides and some natural DNAs having different base pairs composition were studied by means of fluorimetric titrations. The quenching of the fluorescence occurring in the drugs complexed with polynucleotides and DNA is more efficiently induced by the C-G rich regions than by the A-T rich ones. By contrast, the affinity of the drugs for polynucleotides and DNAs in forming the molecular intercalated complexes is correlated mainly with the sequence of purines and pyrimidines in each strand of the duplex polymers, fairly independently of their nature. The regions having an alternate sequence of purines and pyrimidines in each strand are much more preferred than the regions having a continuous sequence; among the various alternate sequences, those having the structure of poly d(A-T) X poly d(A-T) appear to be a little more preferred than the others.
D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D002627:Chemistry, Physical; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism*; D003630:Daunorubicin / Q000378:metabolism*; D004317:Doxorubicin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D004317:Doxorubicin; D004247:DNA; D003630:Daunorubicin