Secretion defect in platelets stored at 4 degrees C.
To understand the functional changes induced by storage, we have examined the adenine nucleotides of platelets stored for 72 hr at 22 degrees C and 4 degrees C. Ten platelet concentrates (PC) were stored at each temperature with five in each group having a final volume of 50 ml and 30 ml. The total ATP and ADP content of platelets decreased following storage in all 4 groups of PC, with the decrease being greater in the PC stored at 22 degrees C than those at 4 degrees C. The mean thrombin secretable ATP + ADP content of platelets from PC stored at 22 degrees C and 4 degrees C were 29.7% and 19.7% of the total content, respectively (p less than 0.001). Thus, cold stored platelets have a higher total content of ATP + ADP but secrete distinctly lesser amounts than 22 degrees C stored platelets. Labeling of the metabolic pool adenylates with 14C-adenine revealed a greater decrease in the adenylate energy charge of the platelets stored at 4 degrees C. The secretion defect demonstrated in cold stored platelets may be related to the inability of these platelets to maintain ATP homeostasis.
D000244:Adenosine Diphosphate / Q000097:blood; D000249:Adenosine Monophosphate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D001792:Blood Platelets / Q000378:metabolism*; D001793:Blood Preservation; D003080:Cold Temperature; D004734:Energy Metabolism; D006801:Humans; D007042:Hypoxanthines / Q000097:blood; D007291:Inosine Monophosphate / Q000097:blood; D010976:Platelet Count; D013917:Thrombin / Q000494:pharmacology
D007042:Hypoxanthines; D007291:Inosine Monophosphate; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D013917:Thrombin