Resolution of two fractions of androgen receptor from mouse kidney.
Putative androgen receptors from mouse kidney were separated and re-chromatographed on DNA-cellulose columns. With either [3H]-dihydrotestosterone or [3H]-testosterone as ligands, two major fractions of androgen receptors were obtained in both elutions from DNA-cellulose columns; these eluted, respectively, at 140-150 mM NaCl (lower-salt) and 180-190 mM NaCl (higher-salt). Therefore, the two major peaks detectable with extracts of mouse kidney result from differences that are intrinsic to the receptor complexes rather than heterogeneity of the DNA-cellulose matrix. These findings are discussed in relation to two forms of androgen receptors that exist in a different proportion in extracts of brain and in relation to other steroid receptors.
D000818:Animals; D002846:Chromatography, Affinity; D013196:Dihydrotestosterone / Q000378:metabolism; D005260:Female; D007668:Kidney / Q000032:analysis*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011944:Receptors, Androgen / Q000302:isolation & purification*; D011987:Receptors, Steroid / Q000302:isolation & purification*; D013739:Testosterone / Q000378:metabolism
D011944:Receptors, Androgen; D011987:Receptors, Steroid; D013196:Dihydrotestosterone; D013739:Testosterone