Failure to demonstrate a role for line Ib tumor-associated surface antigen in the etiology of age-dependent polioencephalomyelitis.
In the preceding paper [Morris et al. (1982), Molec. Immun. 19, 973-982] we demonstrate an associative interaction between the line Ib tumor-associated surface antigen (Ib-TASA) and the Dk/Kk regions of the major histocompatibility complex, i.e. 'altered-self' antigen. We originally hypothesized that age-dependent polioencephalomyelitis (ADPE) occurred as the result of immune recognition of a 'self'-determinant on the 'altered-self' antigen. In this report we used the non-ionic detergent, NP-40, to solubilize Ib cell surface antigens. Although immunization of immunocompetent C58 mice with the soluble NP-40 Ib cell extract afforded protection to lethal tumor challenge, the extract failed to induce ADPE in immunosuppressed mice. Data presented here demonstrate that Ib-TASA is not involved in the etiology of ADPE. The evidence suggests that lactic dehydrogenase virus, which is a silent virus passaged with line Ib leukemia, is the causative agent of the paralytic disease.
D000367:Age Factors; D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D002460:Cell Line; D004679:Encephalomyelitis / Q000209:etiology* / Q000276:immunology / Q000382:microbiology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007772:Lactate dehydrogenase-elevating virus / Q000201:enzymology; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011051:Poliomyelitis / Q000209:etiology* / Q000276:immunology / Q000382:microbiology
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D007770:L-Lactate Dehydrogenase