Plane reconstruction of the structure of mesencephalic tectum from hen embryos in dissociated culture.
The changes in the glial and nerve cells in dissociated cultures of the hen embryo tectum opticum have been studied. The changes observed were of a dual character. The cells in dissociated culture take part in the formation of aggregates of dense clumps comprising astroblasts. In the process of cultivation contacts are established between such aggregates due to intensive radial growth of glial cell processes. Particular attention has been paid to the changes of another type leading to the plane reconstruction of the structure of tectum opticum on the surface of collagen. Due to the directed growth of neuroblasts an organized network is developed with a pronounced 'lamination' involving the neuroblasts with the vertically oriented bodies. These neuroblasts, as a result of continuous differentiation, form the initial parts of apical and basal dendrites, as well as the collaterals participating in the formation of neural components of neuropil. Plane reconstruction of the structure of tectum opticum may be considered as a manifestation of histogenesis in the conditions of tissue culture.
D000818:Animals; D002449:Cell Aggregation; D002478:Cells, Cultured; D002645:Chickens; D003094:Collagen; D005260:Female; D009457:Neuroglia / Q000166:cytology; D009474:Neurons / Q000166:cytology; D013477:Superior Colliculi / Q000166:cytology*
D003094:Collagen