Purification of cytochromes P-448 from beta-naphthoflavone-treated rainbow trout.
Rainbow trout were treated with beta-naphthoflavone and the hepatic microsomal cytochrome P-450 solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate. Chromatography on tryptamine-Sepharose 4B gave a single cytochrome P-450 peak which was further resolved into three components by elution from DEAE-Sepharose. The two main peaks were then chromatographed on hydroxyapatite and a total of four fractions obtained. Two of these fractions had similar properties and significantly metabolized [14C]benzo[a]pyrene in a reconstituted system containing rat cytochrome P-450 reductase. This activity was inhibited by alpha-naphthoflavone but not by metyrapone of SKF-525A. Purified cytochromes P-448 from 3-methylcholanthrene-treated rat had similar spectral properties and activity towards [14C]benzo[a]pyrene suggesting similarities between these forms.
D000818:Animals; D001571:Benzoflavones / Q000494:pharmacology*; D055598:Chemical Phenomena; D002621:Chemistry; D019388:Cytochrome P-450 CYP1A2; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification; D003580:Cytochromes / Q000302:isolation & purification*; D005419:Flavonoids / Q000494:pharmacology*; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D012995:Solubility; D014337:Trout; D019324:beta-Naphthoflavone
D001571:Benzoflavones; D003580:Cytochromes; D005419:Flavonoids; D019324:beta-Naphthoflavone; D003577:Cytochrome P-450 Enzyme System; C017170:Cyp1a2 protein, rat; D019388:Cytochrome P-450 CYP1A2