An improved short-term culture method for human mammary epithelial cells.
An improved short-term culture method for human mammary epithelial cells was developed and the details are presented. Briefly, after the digestion of biopsy tissues with 0.2% collagenase solution for 16 to 18 hr, 4 consecutive fractions were obtained by differential sedimentation. The last fraction, S-4, contained organoids having alveolar- or duct-like structures and gave rise to mammary epithelial cell growth with minimal contamination of fibroblasts.
D001706:Biopsy; D001940:Breast / Q000166:cytology* / Q000473:pathology; D001941:Breast Diseases; D001943:Breast Neoplasms; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000473:pathology; D005260:Female; D006801:Humans