Hepatic gluconeogenesis from alanine following surgery.
To clarify alanine metabolism in the liver with special reference to gluconeogenesis, catheters were placed in hepatic, portal, femoral, and external jugular veins of six male mongrel dogs. A trace amount of 14C-alanine was administered as a single pulse into the external jugular vein on the first postoperative day, and the blood samples were taken from each vein for the subsequent two hours to measure 14C-glucose radioactivity. Cumulative radioactivity after 14C-alanine injection showed that 74 per cent of the radioactivity in whole protein-free serum was that of 14C-glucose. Therefore, it is considered that the metabolic pathway of alanine in the liver after surgery is mainly through gluconeogenesis to glucose. Our in vivo experiment clearly showed that hepatic gluconeogenesis from alanine is one of the important factors related to hyperglycemia after surgery.
D000409:Alanine / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D004285:Dogs; D005943:Gluconeogenesis; D005947:Glucose / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D013514:Surgical Procedures, Operative
D002250:Carbon Radioisotopes; D005947:Glucose; D000409:Alanine