Effect of incubation and translation inhibitors on the transcriptional activity in salivary glands of Chironomus thummi.
Short preincubations of excised salivary glands of Chironomus thummi in synthetic media modify both the activity of uridine uptake into the cells and its incorporation into RNA. The modification of uptake varies with the medium used. Incorporation into total RNA as well as into nucleolar preribosomal RNA is considerably decreased, while incorporation into non-nucleolar RNAs is little affected. When preincubated explanted glands are briefly treated with the protein synthesis inhibitors cycloheximide or anisomycin, the incorporation activity into preribosomal RNA is slightly recovered. This contrasts with the decrease of the labelling of preribosomal glandular RNA, when those drugs are applied in vivo to the larvae.
D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D002683:Chironomidae / Q000235:genetics*; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D004175:Diptera / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D007814:Larva; D008970:Molecular Weight; D009924:Organ Culture Techniques; D014176:Protein Biosynthesis / Q000187:drug effects; D011691:Puromycin / Q000494:pharmacology; D012469:Salivary Glands / Q000502:physiology; D014158:Transcription, Genetic
D003470:Culture Media; D011691:Puromycin; D000841:Anisomycin; D003513:Cycloheximide