Excretion of tumor-associated antigen(s) in the urine of patients with colon carcinoma.
Urine samples collected from patients with colon carcinoma and from normal donors were tested for antigenic activity by the microcomplement-fixation assay. When autologous serum was used as the antibody source, 65.4% (17/26) of the urine samples from patients with colon carcinoma were positive for antigen as opposed to only 10% (2/20) from normal volunteers. Absorption of a representative serum with cultured colon cancer cells completely removed reactivity against its autologous urine. Using this serum to screen urine from colon carcinoma patients, antigenic activity was found in 71.4% (30/46) of the samples; however, only 10% (2/20) of the urine samples from apparently healthy volunteers were positive. Analysis of urine samples collected from three patients before and after resection of their primary colon carcinoma and from nine patients undergoing hyperthermia for liver metastases revealed that two of the patients who had curative surgical procedures had marked decreases in urinary antigen levels by the second postoperative day, while the third patient whose disease was unresectable had no significant decrease. Seven of nine patients with metastatic disease had a greater than fourfold increase in antigen activity after hyperthermia and chemotherapy. These results suggest that tumor-associated antigens were excreted into urine, possibly the result of treatment-caused tumor necrosis. Therefore, assessment of tumor-associated antigen(s) in the urine of patients with colon carcinoma may serve as a marker for response to treatment of this disease.
D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000652:urine*; D003110:Colonic Neoplasms / Q000276:immunology / Q000628:therapy / Q000652:urine*; D003168:Complement Fixation Tests; D005260:Female; D005472:Fluorouracil / Q000627:therapeutic use; D006358:Hot Temperature / Q000627:therapeutic use; D006801:Humans; D008175:Lung Neoplasms / Q000276:immunology / Q000652:urine; D008297:Male; D008545:Melanoma / Q000276:immunology / Q000652:urine; D008875:Middle Aged; D009362:Neoplasm Metastasis; D012878:Skin Neoplasms / Q000276:immunology / Q000652:urine
D000951:Antigens, Neoplasm; D005472:Fluorouracil