Thrombin-induced secretion of serotonin from platelets can occur in seconds.
The platelet release reaction was studied by a new quenched-flow approach. Platelets labeled with 14C-serotonin were reacted for short times (up to 5 sec) with thrombin and then quenched with glutaraldehyde or paraformaldehyde. Serotonin secretion began within 1 sec and was nearly complete by 4 sec. Aggregation recorded by a resistive-particle counter was similarly fast. Therefore, the quenched-flow system reveals that serotonin secretion can occur more rapidly than estimated in earlier studies.
D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D010974:Platelet Aggregation / Q000187:drug effects; D012701:Serotonin / Q000378:metabolism*; D013917:Thrombin / Q000494:pharmacology*; D013997:Time Factors
D012701:Serotonin; D013917:Thrombin