Canine parvovirus: a biochemical and ultrastructural characterization.
A canine virus derived from a diseased dog has been plaque-purified and characterized in detail. Analysis of infected cells demonstrated that virus antigen accumulated in the nucleus at 12 to 24 h post-infection and the cytopathology at the ultrastructural level was diagnostic of a parvovirus infection. The purified virus particles were 23 to 26 nm in diam. and banded at a density 1.44 g/ml in CsCl. Detailed biochemical analysis revealed a single-stranded DNA genome and three structural proteins of mol. wt. 82,300, 67,300 and 63,500. All of the data presented are consistent with the classification of this virus as a parvovirus.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D002213:Capsid / Q000032:analysis*; D002467:Cell Nucleus / Q000382:microbiology*; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000032:analysis*; D004285:Dogs; D010321:Parvoviridae / Q000032:analysis / Q000145:classification / Q000648:ultrastructure*; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000032:analysis*; D015678:Viral Structural Proteins
D000956:Antigens, Viral; D004279:DNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins