A new device for the determination of microsomal cytochrome P-450 in renal tissue preparations from various species contaminated with mitochondria and hemoglobin.
Using a spectrophotometer connected to a microcomputer, the carbon monoxide difference spectrum of renal microsomal dithionite-reduced cytochrome P-450 was measured avoiding the effects of the contaminating mitochondrial cytochromes and hemoglobin by subtracting the CO difference spectrum of a succinate-treated microsomal suspension from that of a dithionite-treated one. By this method, we quantitatively determined the microsomal cytochrome P-450 in kidneys from various species. The absorption peak of the renal microsomal cytochrome P-450 was about 452 nm in rats and about 450 nm in mice, hamsters, rabbits, guinea pigs, dogs, and pigs. Compared with the renal cytochrome P-450 contents in rats, the contents on a g tissue basis were greater in pigs, dogs, and mice and were about the same in hamsters and rabbits. The renal cytochrome P-450 contents in guinea pigs were less than those in rats.
D000818:Animals; D002248:Carbon Monoxide; D006224:Cricetinae; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D004285:Dogs; D005260:Female; D006168:Guinea Pigs; D006454:Hemoglobins / Q000032:analysis; D007668:Kidney / Q000201:enzymology*; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008838:Microcomputers; D008861:Microsomes / Q000201:enzymology*; D008928:Mitochondria / Q000201:enzymology; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013053:Spectrophotometry; D013552:Swine
D006454:Hemoglobins; D002248:Carbon Monoxide; D003577:Cytochrome P-450 Enzyme System