Mouse mammary cells in D-valine medium.
Cells of a mouse mammary epithelial cell line as well as fibroblasts from a mouse mammary explant were severely inhibited from proliferating in a medium in which D-valine was substituted for L-valine. After the first few days in D-valine medium, the number of epithelial cells did not increase despite the fact that a few percent continued to synthesize DNA. The cells did recognize the presence of the D-valine in the medium because cells in D-valine increased in volume and their numbers remained stationary, whereas cells without valine shrank and the cell numbers decreased with time.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects; D004848:Epithelium / Q000502:physiology; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D007700:Kinetics; D008321:Mammary Glands, Animal / Q000502:physiology*; D051379:Mice; D013329:Structure-Activity Relationship; D014633:Valine / Q000494:pharmacology
D014633:Valine