Creatine kinase isoenzyme transitions in muscle grafts of mice.
Histological studies were correlated with developmentally regulated isoenzyme transitions of creatine kinase (CK) in free soleus muscle grafts in mice, 1-25 days after operation. One series of muscles was treated with Marcaine, a myotoxin, in order to promote subsequent regeneration of the muscle. Another set of muscles was mock-treated. In both series of experiments, CK-BB and hybrid CK-MB isoenzymes reappeared transiently as the myoblasts fused and the muscle regenerated. CK isoenzyme transitions were somewhat more pronounced in Marcaine-treated muscle. Nine days after grafting the muscle, specific CK-MM was again present almost exclusively. In this regenerating system, therefore, isoenzyme transitions correlate with histologically detectable regenerative changes, recapitulating the events of embryonic muscle development.
D000818:Animals; D002045:Bupivacaine / Q000494:pharmacology; D003402:Creatine Kinase / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D051379:Mice; D009132:Muscles / Q000201:enzymology / Q000502:physiology* / Q000637:transplantation; D012038:Regeneration / Q000187:drug effects
D007527:Isoenzymes; D003402:Creatine Kinase; D002045:Bupivacaine