The characterization of non-histone proteins whose amounts increase in chromatin from mouse hepatocarcinomas.
A fraction containing liver- and hepatoma-specific non-histone proteins has been isolated from the chromatin of mice. Amino acid analysis of this fraction shows that it contains 16 mol of glutamic acid, 10 mol aspartic acid, 7 mol of both arginine and lysine per 100 mol and contains no cysteine or tyrosine. The proteins in this fraction are strongly associated with DNA and are co-extracted with histones from chromatin with 0.25 M HCl. In chromatin from age-related hepatomas, the amount of this fraction increased six-fold. This increase in concentrations of these chromatin proteins may be associated with changes of chromatin structure necessary to initiate malignant growth in liver cells.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D002843:Chromatin / Q000032:analysis / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000032:analysis; D008099:Liver / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011506:Proteins / Q000032:analysis*
D000596:Amino Acids; D002843:Chromatin; D006657:Histones; D011506:Proteins