Action of vinblastine on myenteric neurones of guinea-pig ileum.
Motor activity of guinea-pig ileum in vitro was monitored by recording isometric tension. Superfusion of the serosal surface with vinblastine (22 microM) increased the motility of ileum but failed to do so in the presence of local anaesthetics and also in preparations depolarised with K+-Tyrode. Atropine (6 microM) or pentolinium (186 microM) reduced the motor response of vinblastine but higher concentration of atropine (60 microM) or pentolinium (930 microM) did not further attenuate the response. Strychnine (58 microM) reversibly blocked the atropine-resistant motor response elicited by vinblastine. It is concluded that vinblastine produces its motor effects by stimulating the non-cholinergic and to some extent cholinergic excitatory neurones of myenteric plexuses.
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D005260:Female; D005769:Gastrointestinal Motility / Q000187:drug effects; D006145:Guanethidine / Q000494:pharmacology; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D009092:Mucous Membrane / Q000187:drug effects; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects*; D009474:Neurons / Q000378:metabolism*; D010425:Pentolinium Tartrate / Q000494:pharmacology; D014747:Vinblastine / Q000494:pharmacology*
D014747:Vinblastine; D001285:Atropine; D010425:Pentolinium Tartrate; D006145:Guanethidine