The roles of different repair mechanisms in the ultraviolet resistance of Micrococcus luteus.
In ultraviolet-irradiated Micrococcus luteus wild type the replication of DNA was not interrupted at every pyrimidine dimer, in contrast to that in ultraviolet-sensitive G7 and some other mutants. The contribution of uninterrupted replication to the ultraviolet resistance of M. luteus proved to be equal to the contributions of excision repair and inducible postreplication repair. It was found that some postreplication gaps could be filled by constitutive pathways of postreplication repair when inducible pathways were suppressed by chloramphenicol. Prolonged treatment with chloramphenicol was shown to block not only inducible repair but also other processes essential for ultraviolet irradiation survival.
D002701:Chloramphenicol / Q000494:pharmacology; D004260:DNA Repair; D004261:DNA Replication; D008837:Micrococcus / Q000528:radiation effects*; D011836:Radiation Tolerance; D014466:Ultraviolet Rays
D002701:Chloramphenicol