High level of tubulin microheterogeneity in the mouse brain.
Using a high resolution isoelectric focusing method, we have demonstrated a high level of tubulin heterogeneity in mouse brain: 21 isotubulins were identified. Determination of the apparent molecular weight and analysis of the peptide map of each isotubulin species allowed us to identify 7 alpha and 14 beta tubulin subunits. Furthermore, variations in this tubulin population during brain development were confirmed; in particular, an increase in the number of beta isotubulins was observed at the beginning of the postnatal period.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D007525:Isoelectric Focusing; D009115:Muridae; D010455:Peptides / Q000378:metabolism; D013237:Stereoisomerism; D014404:Tubulin / Q000378:metabolism*
D010455:Peptides; D014404:Tubulin