Effect of hydrocortisone on the growth of human bone marrow fibroblasts.
Fibroblast-like cells may be grown from human bone marrow. Adherent cultures show a logarithmic phase of growth lasting 6 d. Hydrocortisone (1 x 10(-8) M) decreases cell growth in 40-60% when measured as cell number and DNA synthesis. Fibroblasts bind the hormone by a high affinity, saturable process showing a Kd of 2.2 x 10(-8) M and an average binding capacity of 92 fmol/mg protein. These findings suggest the involvement of glucocorticoids in the regulation and differentiation processes in bone marrow stromal cells.
D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology*; D011965:Receptors, Glucocorticoid / Q000032:analysis
D011965:Receptors, Glucocorticoid; D004247:DNA; D006854:Hydrocortisone