Peripheral nerve cells in culture rich in Schwann cells incorporate and metabolize trans-unsaturated fatty acid (elaidic acid) as well as physiological dis isomer (oleic acid).
A culture of peripheral nerve cells enriched in Schwann cells was obtained from sciatic nerve in normal and demyelinating trembler mutant. These cells incorporated and metabolized a non-physiological trans fatty acid (elaidic aid) as well as the physiological cis isomer (oleic acid). Both acids were incorporated similarly in all lipids studied (phosphatidylcholine was a very potent acceptor) only cholesterol-esters' formation was slightly reduced from elaidic acid. Both acids were partially degradates into sub-units, in turn used for synthesis of new fatty acids. However elaidic acid was less degraded by the cells thus providing more C14:1, C16:1 fatty acids and less cholesterol. The sub-units were also used to provide very long chains, saturated and mono-unsaturated; only synthesis of nervonic acid was at variance when using oleic and elaidic acids. The presence of elaidic acid diminished the elongation-desaturation of essential fatty acids. No major differences were found between control and trembler cells, however cholesterol-esters' synthesis was slightly enhances in the mutant cells, when using both acids.
D000818:Animals; D046508:Culture Techniques; D050356:Lipid Metabolism; D051379:Mice; D008818:Mice, Neurologic Mutants; D009474:Neurons / Q000166:cytology; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism*; D012583:Schwann Cells / Q000166:cytology*; D012584:Sciatic Nerve / Q000166:cytology*; D013237:Stereoisomerism
D009829:Oleic Acids; D019301:Oleic Acid; C011459:elaidic acid