Aspects of non-enzymatic glycosylation of serum proteins in relation to different glucose loads.
Non-enzymatic glycosylation of serum proteins (GSP) has been studied in control subjects (n = 10) and diabetic patients (n = 12) during different types of glucose loads. In all cases basal GSP, determined after serum dialysis, gave values which were lower than those found before removing glucose. In the diabetics, moreover, they were significantly higher than in the controls. During OGTT and intravenous glucose infusion prolonged for 5 hours, in both groups, GSP, assayed before dialysis, was found be higher than in basal conditions, while after dialysis values were similar to fasting levels. It can be concluded that also after dialysis, to remove free glucose from sample, GSP is correlated with carbohydrate intolerance but the phenomenon is not induced by either brief hyperglycemic peaks or by high glycemic concentrations maintained for 5 hours.
D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D005947:Glucose / Q000008:administration & dosage*; D005951:Glucose Tolerance Test; D006801:Humans
D001786:Blood Glucose; D001798:Blood Proteins; D005947:Glucose