Chemical nature of mouse antibodies homologous to the 3-pyridylazo group: the fine specificities of hybridoma and serum antibodies.
Rabbit antibodies which bind aromatic annular nitrogen-containing haptens exhibit a specificity wherein such nitrogens are distinguished from the closely related aromatic CH group. The mouse hybridoma system was used to extend this work producing hybridoma antibodies homologous to the 3-pyridylazo group. Fine specificity mapping by double antibody radioimmunoassay revealed differences among the individual hybridomas, as well as a greater resemblance of mouse serum antibodies to rabbit serum antibodies than to hybridoma antibodies. Quantitative structure-activity relationships applying the parameters of hapten molar refractivity had hydrophobicity were used to help elucidate the types of intermolecular forces involved in the interaction of pyridine derivatives with the antibodies. The results are consistent with the interpretation that pyridine binding to antibody does not involve desolvation.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001391:Azo Compounds / Q000276:immunology*; D002460:Cell Line; D006241:Haptens / Q000276:immunology; D006825:Hybridomas / Q000276:immunology*; D051379:Mice; D009101:Multiple Myeloma / Q000276:immunology; D011725:Pyridines / Q000276:immunology*; D013329:Structure-Activity Relationship
D000911:Antibodies, Monoclonal; D001391:Azo Compounds; D006241:Haptens; D011725:Pyridines