Isolation and partial characterization of rat duodenal-gland (Brunner's-gland) mucus glycoprotein.
A mucus glycoprotein was isolated from the duodenal glands of the rat and purified by repeated density-gradient centrifugation. The characterized glycoprotein is unique to the mucous cells of the duodenal glands and is not present in parts of the small intestine devoid of these glands. The chemical composition of the purified glycoprotein is characteristic for glycoproteins of the mucin-type. Its protein content is relatively high and amount to 35% by weight. No neuraminic acid and little sulphate (2%) is present. Evidence is presented that the native glycoprotein is built up from subunits held together via disulphide bridges in a non-glycosylated region of the protein core.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002011:Brunner Glands / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D004386:Duodenum / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification*; D008297:Male; D009093:Mucus / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D000596:Amino Acids; D006023:Glycoproteins