Binding of human immunoglobulin M to Staphylococcus aureus bearing protein A.
Human immunoglobulin M binds to protein A-bearing Staphylococcus aureus. This binding is specific for protein A since it is inhibited by rabbit Fab against Staphylococcal protein A. Analysis of the IgM binding data gives an apparent KD of 0.94 nM and a maximum capacity of approximately 2 X 10(3) binding sites of IgM per bacteria.
D000818:Animals; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007140:Immunoglobulin Fab Fragments; D007075:Immunoglobulin M / Q000378:metabolism*; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000276:immunology / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000378:metabolism; D013997:Time Factors
D007140:Immunoglobulin Fab Fragments; D007075:Immunoglobulin M; D013205:Staphylococcal Protein A