Phenolic components of the primary cell wall. Feruloylated disaccharides of D-galactose and L-arabinose from spinach polysaccharide.
1. Cell walls from rapidly growing cell suspension cultures of Spinacia oleracea L. contained ferulic acid and p-coumaric acid esterified with a water-insoluble polymer. 2. Prolonged treatment with trypsin did not release may feruloyl esters from dearabinofuranosylated cell walls, and the polymer was also insoluble in phenol/acetic acid/water (2:1:1, w/v/v). 3. Treatment of the cell walls with the fungal hydrolase preparation "Driselase' did liberate low-Mr feruloyl esters. The major esters were 4-O-(6-O-feruloyl-beta-D-galactopyranosyl)-D-galactose and 3?-O-feruloyl-alpha-L-arabinopyranosyl)-L-arabinose. These two esters accounted for about 60% of the cell-wall ferulate. 4. It is concluded that the feruloylation of cell-wall polymers is not a random process, but occurs at very specific sites, probably on the arabinogalactan component of pectin. 5. The possible role of such phenolic substituents in cell-wall architecture and growth is discussed.
D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D003373:Coumaric Acids / Q000032:analysis; D004187:Disaccharides / Q000032:analysis*; D004590:Electrophoresis, Paper; D005656:Fungal Proteins; D006026:Glycoside Hydrolases / Q000494:pharmacology; D010070:Oxalates / Q000494:pharmacology; D019815:Oxalic Acid; D019800:Phenol; D010636:Phenols / Q000494:pharmacology; D010944:Plants / Q000187:drug effects / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology
D003373:Coumaric Acids; D004187:Disaccharides; D005656:Fungal Proteins; D010070:Oxalates; D010636:Phenols; D011134:Polysaccharides; D019800:Phenol; D019815:Oxalic Acid; C004999:ferulic acid; D006026:Glycoside Hydrolases; C035212:driselase; D014357:Trypsin