Clastogenic activity of bile acids and organic acid fractions of human feces.
Chloroform extracts of fecal material from 4 subjects on normal mixed western diets were fractionated to obtain an acid fraction and a hexane extract containing neutrals and bases. The acid fraction from at least 2 of the donors induced an elevated frequency of chromosomal aberrations and exchanges in Chinese hamster ovary (CHO) cells. Since acid steroids are expected to be present in the acid fraction, 5 bile acids were assayed for clastogenic activity in CHO cells. Ursodeoxycholic acid induced chromosomal aberrations and exchanges, and this effect was enhanced by the addition of a microsomal S9 mix. However, the enhancement is probably due to physical factors rather than to enzymatic activity.
D000143:Acids / Q000032:analysis*; D000818:Animals; D001647:Bile Acids and Salts / Q000009:adverse effects* / Q000032:analysis / Q000378:metabolism; D005591:Chemical Fractionation; D002869:Chromosome Aberrations; D006224:Cricetinae; D005243:Feces / Q000032:analysis*; D006801:Humans; D000432:Methanol / Q000009:adverse effects / Q000032:analysis; D009152:Mutagenicity Tests; D014580:Ursodeoxycholic Acid / Q000009:adverse effects / Q000378:metabolism
D000143:Acids; D001647:Bile Acids and Salts; D014580:Ursodeoxycholic Acid; D000432:Methanol