Production of direct agglutinins by the combination of incomplete antibodies to protein A-bearing Staphylococcus aureus cells.
A novel method is described for the production of saline-reacting antibodies from "incomplete' blood grouping sera. IgG anti-D is combined to protein A-bearing Staphylococcus aureus (SA), forming a polyvalent anti-D assembly. The SA-anti-D has a high titre in saline and is stable on 4 degrees C storage and lyophilisation. The material was successful in Rh(D) typing cells sensitised in vitro with various antibodies. Saline-reacting SA-anti-Kell and SA-anti-C were also produced.
D000373:Agglutinins / Q000096:biosynthesis*; D000906:Antibodies / Q000378:metabolism*; D000918:Antibody Specificity; D004356:Drug Storage; D006801:Humans; D007626:Kell Blood-Group System; D013205:Staphylococcal Protein A / Q000276:immunology*
D000373:Agglutinins; D000906:Antibodies; D007626:Kell Blood-Group System; D013205:Staphylococcal Protein A