Human colorectal tumours in short-term organ culture. A stathmokinetic study.
Short-term organ culture, using a technique to preserve epithelial/stromal interaction and metabolism, is a useful technique for carrying out kinetic studies on human colorectal carcinoma and adjacent normal mucosa, providing initial perturbations of proliferative indices are allowed to settle. Tumours require 3.0 micrograms/ml vincristine for complete metaphase arrest compared with mucosa, which needs 0.5 microgram/ml, a 6-fold difference. Using a stathmokinetic technique, the birth rate of tumour cells is 10.21 cells/1000 cells per hr, compared with 7.73 cells/1000 cells per hr for mucosa, a statistically significant difference (P less than 0.01).
D000230:Adenocarcinoma / Q000473:pathology*; D002455:Cell Division; D003110:Colonic Neoplasms / Q000473:pathology*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology*; D007700:Kinetics; D008677:Metaphase; D009924:Organ Culture Techniques; D012004:Rectal Neoplasms / Q000473:pathology*; D014750:Vincristine / Q000494:pharmacology
D014750:Vincristine