[Studies on the effect of low concentrations of thiocyanate on the protein human serum albumin].
By means of fluorescence and absorption measurements the authors studied the interaction of the protein human serum albumin with relatively low concentrations (up to 5 mmol/l at the most) of thiocyanate ions. Such concentrations lead to a slight quenching of the tryptophan fluorescence in human serum albumin. The effect of thiocyanate is not due to an overall action on the protein (as sometimes observed with higher thiocyanate concentrations), but to a local interaction of the fluorophore tryptophan with the neighbouring anion-binding site which is occupied by thiocyanate.
D001665:Binding Sites; D006801:Humans; D007211:Indoles / Q000097:blood; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013861:Thiocyanates / Q000097:blood*; D014364:Tryptophan / Q000097:blood
D007211:Indoles; D012709:Serum Albumin; D013861:Thiocyanates; C024553:sodium thiocyanate; D014364:Tryptophan
Studien zur Beeinflussung des Proteins Humanserumalbumin durch Thiocyanationen in geringen Konzentrationen.