Correlation of pancreatic blood flow and high-energy phosphates during experimental pancreatitis.
A dog model was used to measure the hemodynamic changes occurring during acute pancreatitis induced by intraductal injection of fresh trypsin-bile-blood mixture. Pancreatic blood flow was measured with 15-micrometer radioactive microspheres. Measurements of pancreatic adenosine triphosphate (ATP) and creatine phosphate (CP) were made under normal conditions and during acute hemorrhagic pancreatitis. Basal ATP and CP concentrations were 5.82 +/- 0.25 and 5.30 +/- 0.31 mmol/g wet tissue, respectively. Hemorrhagic pancreatitis was characterized by a severe reduction in pancreatic blood flow, followed by a 45% fall of ATP and a 70% lowering of CP. These results suggest that inadequate pancreatic tissue perfusion during acute pancreatitis results in a marked depletion of high-energy phosphate stores. We suspect this energy depletion reflects the progression of the disease from edematous to hemorrhagic pancreatitis and causes irreversible damage of pancreatic tissue.
D000208:Acute Disease; D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D004285:Dogs; D006439:Hemodynamics; D006470:Hemorrhage / Q000139:chemically induced / Q000378:metabolism; D010179:Pancreas / Q000098:blood supply*; D010195:Pancreatitis / Q000139:chemically induced / Q000378:metabolism*; D010725:Phosphocreatine / Q000032:analysis*; D012039:Regional Blood Flow
D010725:Phosphocreatine; D000255:Adenosine Triphosphate