Detection of lymphocytic Fc receptors with 125I-labelled protein A.
An attempt is made to improve the methodology for the detection of lymphocytic Fc receptors, using 125I-labelled protein A from Staphylococcus Aureus (125ISpA) and different immunoglobulins G such as human monomer IgG, Fc antiserum and swine Fc+F'c antiserum. The working conditions and sources of error that might affect the results were established. This methodology was applied to the study of leukemia, chronic hepatitis, allergic diseases and normal cases. In the chronic lymphoid leukemia cases the determinations were repeated at different clinico-biological stages. The values obtained in these cases have always been higher than normal. This method may be used for rapid determination of the presence of Fc receptors; its application in the clinic would furnish useful data. It still remains to be established whether determination of these lymphocytic receptors might contribute data that can be corroborated by the response to the immunodepressor treatment applied in some cases.
D006801:Humans; D007457:Iodine Radioisotopes; D008214:Lymphocytes / Q000276:immunology*; D011961:Receptors, Fc / Q000032:analysis*; D013205:Staphylococcal Protein A
D007457:Iodine Radioisotopes; D011961:Receptors, Fc; D013205:Staphylococcal Protein A