Iron-induced DNA damage and synthesis in isolated rat liver nuclei.
Incubation of iron with isolated rat liver nuclei stimulated fragmentation of single-stranded DNA, incorporation of [3H]thymidine into DNA and the binding of 59Fe to DNA. FeCl2 was about twice as active as FeCl3. Lipid peroxidation took place in nuclei incubated with FeCl2, but not with FeCl3. Generation of reactive forms of oxygen was required for iron-mediated DNA damage, but evidence for direct interaction of reactive oxygen with DNA was not found. Apparent adducts of iron bound to DNA seemed to be formed by an enzymic mechanism.
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002712:Chlorides; D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D005290:Ferric Compounds / Q000494:pharmacology*; D005296:Ferrous Compounds / Q000494:pharmacology*; D066298:In Vitro Techniques; D007501:Iron / Q000494:pharmacology*; D050356:Lipid Metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D013942:Thymine Nucleotides / Q000378:metabolism
D002712:Chlorides; D004277:DNA, Single-Stranded; D005290:Ferric Compounds; D005296:Ferrous Compounds; D013942:Thymine Nucleotides; D004247:DNA; D007501:Iron; C024157:thymidine 5'-triphosphate; C029451:ferrous chloride; C024555:ferric chloride