L-Phenylalanine inhibition of human alkaline phosphatases with p-nitrophenyl phosphate as substrate.
With p-nitrophenyl phosphate as the substrate, there reportedly is no organ-specific inhibition of alkaline phosphatase (EC 3.1.3.1) activity by L-phenylalanine. However, we found that at pH 10.0, with p-nitrophenyl phosphate as the substrate, L-phenylalanine obviously inhibits the alkaline phosphatase isoenzyme from human placenta, whereas there is little if any inhibition of the isoenzyme from human intestine. Because of the differing effects of substrates (p-nitrophenyl phosphate and phenyl phosphate) and their enzymic products (p-nitrophenol and phenol) for L-phenylalanine action on the placental alkaline phosphatase isoenzyme, we suggest that the isoenzyme--inhibitor--substrate complex and the effect of released phosphate on L-phenylalanine inhibition of the isoenzyme activity differ from each other.
D000328:Adult; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000201:enzymology; D009596:Nitrophenols / Q000378:metabolism*; D010755:Organophosphates; D009943:Organophosphorus Compounds / Q000378:metabolism*; D010649:Phenylalanine / Q000494:pharmacology*; D010920:Placenta / Q000201:enzymology
D009596:Nitrophenols; D010755:Organophosphates; D009943:Organophosphorus Compounds; C008644:nitrophenylphosphate; D010649:Phenylalanine; D000469:Alkaline Phosphatase; C005445:triphenyl phosphate