Stoichiometry of oxygen consumption in the biosynthesis of isopenicillin from a tripeptide.
The biosynthesis of isopenicillin N from delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine in a cell-free system has been correlated wih O2 consumption by two methods, involving the use of an oxygen-electrode and an n.m.r. spectrometer respectively. The results are consistent with a 1 : 1 stoichiometric ratio for the dioxygen consumed to the isopenicillin N formed.
D002474:Cell-Free System; D004566:Electrodes; D009682:Magnetic Resonance Spectroscopy; D008722:Methods; D009842:Oligopeptides / Q000378:metabolism*; D010101:Oxygen Consumption; D010406:Penicillins / Q000096:biosynthesis*
D009842:Oligopeptides; D010406:Penicillins; C011638:5-(2-aminoadipyl)cysteinylvaline; C006414:penicillin N