[Analysis of the mast cell population in excitation and blockade of the anticoagulant system].
Stimulation of the anticoagulation system with the structural analogs of alpha-thrombin, DPP-alpha-thrombin and prothrombin I having no proteolytic activity leads, as is the case with alpha-thrombin, to a decrease in the heparin content in mast cells and to a concurrent rise in the blood level of the complex compounds of heparin and blood proteins. Morphometry disclosed a substantial shift of mast cell population towards the light, heparin-free forms. In the experimental animals, the index of mast cell saturation with heparin dropped below one. Heparin release by mast cells proceeded by the merocrine secretion type, largely at the expense of granulolysis, with the cell integrity being preserved. Degranulation was effected via the strong form. Intravenous injection to the animals of alpha-thrombin in the presence of the anticoagulation system blockade with chlorpromazine produced no similar changes in the mast cell population.
D000818:Animals; D002746:Chlorpromazine; D004792:Enzyme Precursors / Q000494:pharmacology; D005342:Fibrinolysis / Q000187:drug effects; D006493:Heparin / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000166:cytology* / Q000378:metabolism; D011516:Prothrombin / Q000494:pharmacology; D051381:Rats; D013917:Thrombin / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology
D004792:Enzyme Precursors; D011516:Prothrombin; D006493:Heparin; C012007:prethrombins; D013917:Thrombin; C027962:diisopropylphosphoryl-thrombin; D002746:Chlorpromazine
Analiz tuchnokletochnoĭ populiatsii pri vozbuzhdenii i blokade protivosvertyvaiushcheĭ sistemy.