The preparation of protein A-gold complexes with 3 nm and 15nm gold particles and their use in labelling multiple antigens on ultra-thin sections.
The preparation of a protein A-gold complex (pAg3) using 3 nm gold particles and its application for labelling of intracellular antigens on thin sections is reported. The 3 nm gold particle is the smallest metal particle currently available for cytochemistry and permits a higher resolution of the pAg technique. Furthermore, it can be used in double labelling experiments in conjunction with a pAg complex prepared from 15nm gold particles. For double labelling, the pAg3 complex must be used for staining of the first antigen since otherwise a non-specific co-labelling of the two pAg complexes results.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D003102:Colloids; D006046:Gold; D006651:Histocytochemistry / Q000379:methods*; D051381:Rats; D013205:Staphylococcal Protein A
D000941:Antigens; D003102:Colloids; D013205:Staphylococcal Protein A; D006046:Gold