400 MHz NMR study on the C-terminal fragment 21-28 of vasoactive intestinal peptide.
A conformational study of the C-terminal fragment 21-28 of vasoactive intestinal peptide (VIP) was carried out by high resolution NMR spectroscopy. All spectral data were recorded with a Brüker 400 MHz spectrometer. The correct assignment of peaks was determined by specific homonuclear decoupling and by titration. The chemical shifts of amide protons were measured as a function of temperature in order to detect the presence of intramolecular hydrogen bonds. A tridimensional structure is proposed for the peptide.
D005768:Gastrointestinal Hormones / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D011487:Protein Conformation; D013696:Temperature; D014660:Vasoactive Intestinal Peptide / Q000032:analysis*
D005768:Gastrointestinal Hormones; D014660:Vasoactive Intestinal Peptide