[Influence of follicular cells on energy metabolism of oocytes in growing mice].
To investigate to what extent follicle cells can influence the energy metabolism of growing oocytes and thus regulate their development, we have compared incorporation of 3H-leucine into proteins of denuded and follicle enclosed oocytes as related to the presence of different energy metabolites in the culture medium. The results indicate that a) oocytes have no endogenous reserves and that are dependent on the presence of exogenous pyruvate for their viability; b) follicular cells possess an energy reserves that can be utilized by the germinal cells; c) follicular cells mediate the utilization of exogenous glucose by growing oocytes. We can speculate that follicular cells transform glucose in pyruvate, utilizable by isolated oocytes, or they stimulate glucose uptake and glycolysis of the oocytes, as suggested by metabolic cooperativity for 2deoxy-D-glucose.
D000818:Animals; D004734:Energy Metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D051379:Mice; D009865:Oocytes / Q000378:metabolism; D006080:Ovarian Follicle / Q000166:cytology*; D010053:Ovary / Q000254:growth & development*; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid
D011773:Pyruvates; D019289:Pyruvic Acid; D007930:Leucine; D005947:Glucose
Influenza delle cellule follicolari sul metabolismo energetico degli ovociti di topo in accrescimento.