5,6-Saturated thymine lesions in DNA: production by ultraviolet light or hydrogen peroxide.
Thymine analogs with saturated 5-6 bonds are important types of DNA damage that are recognized by the DNA N-glycosylase activity of E. coli endonuclease III. Seeking agents which could preferentially form 5,6-hydrated thymine residues in duplex DNA both in vivo and in vitro, we exposed purified duplex DNA to 325- or 313-nm light; however, after such exposure pyrimidine dimers greatly predominated over 5,6-hydrated thymine. Hydrogen peroxide, on the other hand, formed significant numbers of endonuclease III-sensitive sites in vitro which were not apurinic/apyrimidinic lesions and thus were likely to be 5,6-hydrated thymines.
D001435:Bacteriophages; D004279:DNA, Viral / Q000528:radiation effects; D006861:Hydrogen Peroxide; D007700:Kinetics; D011549:Pseudomonas; D013941:Thymine / Q000031:analogs & derivatives* / Q000528:radiation effects; D014466:Ultraviolet Rays
D004279:DNA, Viral; D006861:Hydrogen Peroxide; D013941:Thymine