[Spectroscopic evidence of the interaction of the dye pyronine G with polynucleotides].
The interaction of pyronine G with mono- (AMP) and polynucleotides, i. e. poly(A) and poly(U), was studied at different pH values and temperatures, both in the visible and near UV ranges. It was found that pyronine G interacts only with purine type polynucleotides at neutral and alkaline pH. This was confirmed by the appearance of a new dye absorption band in the visible range and the existence of a hypochromic effect in the UV. The spectroscopical evidence for the formation of pyronine G--AMP or poly(U) complex has not been found at any conditions. With these results in view, possible binding mechanism of pyronine G to polynucleotides are discussed.
D000249:Adenosine Monophosphate; D055598:Chemical Phenomena; D002621:Chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011061:Poly A; D011072:Poly U; D011754:Pyronine; D013053:Spectrophotometry; D013696:Temperature; D014966:Xanthenes
D014966:Xanthenes; D011061:Poly A; D011072:Poly U; D000249:Adenosine Monophosphate; D011754:Pyronine
Spektral'nye proiavleniia vzaimodeĭstviia krasitelia pironina G s polinukleotidami.