A circular-dichroism study of epidermolytic toxins A and B from Staphylococcus aureus.
The far-u.v. circular-dichroism spectra of the two epidermolytic toxins was analysed into fractional contributions of 0.09 helix and 0.46 beta-sheet to each toxin structure. Trifluoroethanol perturbation caused an initial increase in dichroic absorption at 205 nm and then a change characterized as a beta-sheet-to-alpha-helix transition. The intense near-u.v. spectra suggested that the toxins have unusually rigid, though different, aromatic-side-chain arrangements.
D001427:Bacterial Toxins; D002942:Circular Dichroism; D005083:Exfoliatins; D013211:Staphylococcus aureus / Q000032:analysis*; D014270:Trifluoroethanol; D014364:Tryptophan / Q000032:analysis
D001427:Bacterial Toxins; D005083:Exfoliatins; D014270:Trifluoroethanol; D014364:Tryptophan