Hydrolysis of triacylglycerol emulsions by lingual lipase. A microscopic study.
The effect of lingual lipase on four different triacylglycerol emulsions was observed by light microscopy at pH 5-6. The extent of hydrolysis on the microscope slide was determined with the aid of radioactive emulsions or by analyzing the products by gas-liquid chromatography. Artificial emulsions that had been stabilized with amphiphilic lipids gradually coalesced during the unstirred lipase reactions. Gum arabic-stabilized emulsions and human milk fat droplets did not stick to each other or coalesce during lingual lipase hydrolysis. No visible liquid-crystalline product phases, as are seen with pancreatic lipase (Patton, J.S. and Carey, M.C. (1979) Science 204, 145-148), were observed with lingual lipase. The products of lingual lipase activity, protonated fatty acid and diacylglycerol, appear to remain dissolved in the oil phase of the triacylglycerol particle.
D000818:Animals; D005227:Fatty Acids / Q000032:analysis; D005614:Freeze Fracturing; D008049:Lipase / Q000378:metabolism*; D008854:Microscopy, Electron; D008895:Milk, Human / Q000378:metabolism; D051381:Rats; D014059:Tongue / Q000201:enzymology*; D014280:Triglycerides / Q000378:metabolism*
D005227:Fatty Acids; D014280:Triglycerides; D008049:Lipase