Agglutination of isolated rat bladder cells by lectins after administration of N-butyl-N-(4-hydroxybutyl) nitrosamine.
Lectins, concanavalin A (Con A), wheat germ agglutinin (WGA), and Ricinus communis agglutinin (RCA) were used for agglutination of isolated rat bladder cells after administration of N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN). The results showed that WGA and RCA, as well as Con A, agglutinated rat bladder cells during the early stage of bladder carcinogenesis. The agglutinations by the 3 kinds of lectins were different; WGA and Con a agglutinated only BBN-treated cells but RCA agglutinated both BBN-membrane alterations during the early phase of bladder carcinogenesis.
D000371:Agglutination; D000818:Animals; D002085:Butylhydroxybutylnitrosamine / Q000633:toxicity*; D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology*; D008297:Male; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology; D009602:Nitrosamines / Q000633:toxicity*; D037121:Plant Lectins; D011230:Precancerous Conditions / Q000139:chemically induced / Q000276:immunology; D051381:Rats; D001743:Urinary Bladder / Q000276:immunology*; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000276:immunology*
D037102:Lectins; D009602:Nitrosamines; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D003208:Concanavalin A; D002085:Butylhydroxybutylnitrosamine