Studies on the preparation of conjugates of leukotriene C4 with proteins for development of an immunoassay for SRS-A (1).
Leukotriene C4 (LTC4) has been conjugated with Bovine Serum Albumin (BSA) and Hemocyanin from Giant Keyhole Limpets (KLH) using 1,5-difluoro-2,4-dinitrobenzene as coupling agent. A second LTC4-KLH conjugate was prepared using a new bifunctional coupling agent, 6-N-maleimidohexanoic acid chloride. Conjugates of some representative LTC4 component parts; S-p-chlorophenacylglutathione with BSA and of 2,4(E),6,9(Z)-pentadecatetraen-1-ol with BSA were also prepared.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004139:Dinitrofluorobenzene / Q000031:analogs & derivatives; D006433:Hemocyanins; D008974:Mollusca; D011863:Radioimmunoassay / Q000379:methods; D013189:SRS-A / Q000032:analysis*; D012710:Serum Albumin, Bovine
D013189:SRS-A; D012710:Serum Albumin, Bovine; D006433:Hemocyanins; D004139:Dinitrofluorobenzene; C002041:1,5-difluoro-2,4-dinitrobenzene