DNA degradation in chinese hamster ovary cells after exposure to hyperthermia.
Chinese hamster ovary cells grown in suspension showed a progressive reduction in the size of their nuclear DNA to 50 to 60S fragments after hyperthermia (43-48 degrees). This DNA degradation was not a homogeneous response but was observed only in cells incapable of attaching to a substratum after acute heating. The DNA degradation was associated with the inability of cells to exclude the vital stain, trypan blue. The degradation process appeared to be a result of nucleolytic enzyme digestion which accompanies cell necrosis. A similar phenomenon was observed in heated monolayer cells but only after significantly greater time-temperature exposures. Our results show that cellular subpopulations can be separated after hyperthermia and that these subpopulations are biochemically distinct and characterized by different viability.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D006358:Hot Temperature; D007700:Kinetics; D010053:Ovary
D004247:DNA