[Method of plasma acetate determination by gas chromatography after ethanol administration in man (author's transl)].
Plasma acetate is extracted using ethanol in alkaline medium, in presence of internal standard. After concentration of the hydroalcoholic phase, the chromatographic analysis is performed utilizing 10% SP 1200 and 1% H3PO4 on chromosorb W. The linearity of the method has been tested from 0.05 to 10 mM/I, its reproducibility is +/- 3.5% for levels ranging from 0.1 to 10 mM/I and +/- 10% for those smaller than 0.1 mM/I. This method is sensitive enough to assess the physiological acetate levels and those obtained after ethanol biotransformation.
D000085:Acetates / Q000097:blood*; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas / Q000379:methods; D000431:Ethanol / Q000378:metabolism*; D006801:Humans; D013997:Time Factors
D000085:Acetates; D000431:Ethanol
Mise au point d'une méthode de dosage par chromatographie en phase gazeuse de l'acétate plasmatique après administration d'éthanol chez l'homme.