Determination of dihydroergot alkaloids in serum by radioimmunoassay.
A radioimmunoassay for dihydroergotoxine was developed using antiserum frm the rabbit injected with a dihydromethylergometrine-bovine serum albumin conjugate. The antiserum was found to be highly specific to dihydroergot alkaloids. The minimum detectable amount of dihydroergotoxine methanesulfonate with this assay system was about 100 pg/ml in serum. Practical applicability of the assay system was proved by measuring dog serum concentrations after oral administration of dihydroergotoxine methanesulfonate.
D000284:Administration, Oral; D000818:Animals; D004088:Dihydroergotoxine / Q000008:administration & dosage / Q000097:blood* / Q000276:immunology; D004285:Dogs; D005260:Female; D007106:Immune Sera / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*
D007106:Immune Sera; D004088:Dihydroergotoxine