Enhancement of mitogen-induced human lymphocyte transformation by diiron enneacarbonyl in serum-free medium.
Diiron enneacarbonyl, colloidal iron particles used for removing monocytes, enhanced the 3H-thymidine uptake into the mitogen-stimulated human lymphocytes in serum-free medium, whereas neither silica particles, latex particles, carbonyl iron powder nor iron pentacarbonyl did. Because monocytes had no influence on the enhancing effect or diiron enneacarbonyl did not bind to PHA, it was suggested that the enhancing effect was performed by diiron enneacarbonyl directly acting on the mitogen-activated lymphocytes in serum-free medium.
D003470:Culture Media; D006801:Humans; D066298:In Vitro Techniques; D007501:Iron / Q000494:pharmacology*; D054354:Iron Carbonyl Compounds; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects*; D008934:Mitogens / Q000494:pharmacology*; D009000:Monocytes / Q000276:immunology; D009942:Organometallic Compounds; D010835:Phytohemagglutinins / Q000494:pharmacology
D003470:Culture Media; D008934:Mitogens; D009942:Organometallic Compounds; D010835:Phytohemagglutinins; D054354:Iron Carbonyl Compounds; D007501:Iron