Purification, characterization, and comparison of extracellular hemoglobins in coelomic fluid and blood vessel plasma of Travisia japonica (Polychaeta, Annelid).
1. Two kinds of extracellular gigantic hemoglobins in blood vessel plasma and in coelomic fluid of Travisia japonica (polychaeta, annelid) were purified. 2. Their properties including electron microscopic observation, gel filtration, amino acid analysis, SDS polyacrylamide gel electrophoresis, and immunological properties were studied and compared. No significant difference was found between them. 3. They had a mol. wt of ca. 3 X 10(6) and a shape consisting of 12 subunits with a mol. wt of ca. 2.4 X 10(5) arranged in two superimposed hexagonal rings with a diameter of 22-25 nm and a thickness of 15-16 nm, as for other annelid gigantic hemoglobins described in the literatures.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D006454:Hemoglobins / Q000302:isolation & purification*; D046911:Macromolecular Substances; D008970:Molecular Weight; D011077:Polychaeta / Q000032:analysis*
D000596:Amino Acids; D006454:Hemoglobins; D046911:Macromolecular Substances