Determination of tripamide and its metabolites in plasma, red blood cells and urine by high-performance liquid chromatography.
A procedure for the determination of tripamide and its hydroxylated metabolites in plasma, red blood cells and urine by reversed-phase high-performance liquid chromatography is described. The concentrations in red blood cells showed a monophasic decline and the half-life was 9.5 h. The concentration in red blood cells was markedly higher than that in plasma, showing that 95-98% of the drug is present in whole blood, after a dose of tripamide (90 mg) in man. The specificity and sensitivity of this procedure appear to be satisfactory for pharmacokinetic studies.
D000328:Adult; D000959:Antihypertensive Agents / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000032:analysis*; D006207:Half-Life; D006801:Humans; D007211:Indoles / Q000032:analysis* / Q000097:blood; D007700:Kinetics; D008297:Male; D008875:Middle Aged
D000959:Antihypertensive Agents; D007211:Indoles; C031282:tripamide