High-performance liquid chromatographic determination of proglumide in plasma.
A rapid and sensitive method for determining the anticholinergic agent, proglumide, in plasma by high-performance liquid chromatography is described. Samples were acidified with hydrochloric acid and extracted with chloroform. The dried extract was resolved in chloroform and chromatographed on an adsorption chromatographic column using a mobile phase of chloroform-methanol (24:1) on a high-performance liquid chromatograph equipped with a UV absorbance detector (240 nm). The detection limit for proglumide was 0.05 microgram/ml.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005973:Glutamine / Q000031:analogs & derivatives*; D006801:Humans; D008297:Male; D011377:Proglumide / Q000097:blood*; D013997:Time Factors
D005973:Glutamine; D011377:Proglumide