Effect of phenobarbital on cytoplasmic and nuclear distribution of 3H-arginine in rat liver.
Rat liver derived from animals receiving phenobarbital (80 mg/kg intraperitoneally) exhibited an increase in the cytoplasmic concentration of radioactive amino acid (initially injected as arginine) as early as 1 hour after the injection. This increase in uptake resulted in an unequal distribution of the radioactivity between the cytoplasmic and nuclear compartments; while other organelles retained their normal (per cent) allotment of the radioactivity. The cytoplasmic and nucleoplasmic protein profiles from gel chromatography show quantitative changes that may be due to increased synthesis and migration of a cytoplasmic protein to the nucleus where its nuclear function remains to be examined more thoroughly.
D000818:Animals; D001120:Arginine / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D010634:Phenobarbital / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000378:metabolism; D013997:Time Factors
D011506:Proteins; D001120:Arginine; D010634:Phenobarbital