[Proteolytic splitting of proteins from the cortex and nuclear zones of the crystalline lens].
The proteolytic attacking capacity by pronase, a proteolytic wide-range enzyme, has been studied in soluble and insoluble proteins from the cortex and nuclear lens zones of young (aged 1.5-2 years) and old (aged 6-12 years) animals. It is established that proteolysis stability of proteins from the cortex lens zone in young and old animals is the same. The soluble proteins of the lens nucleus are attacked by the enzyme considerably weaker in old animals than in young animals; insoluble proteins in the process of ageing get more sensitive to the pronase action. Post-translation modifications of proteins--deamidation, intra- and intermolecular crosslinkage--may be the reason of changes in the pronase attacking capacity of proteins in the nuclear lens zone of old animals.
D000375:Aging; D000818:Animals; D002417:Cattle; D003459:Crystallins / Q000302:isolation & purification*; D007908:Lens, Crystalline / Q000254:growth & development*; D010446:Peptide Fragments / Q000032:analysis; D011402:Pronase; D011499:Protein Processing, Post-Translational
D003459:Crystallins; D010446:Peptide Fragments; D011402:Pronase
Proteoliticheskoe rasshcheplenie belkov iz korkovoĭ i iadernoĭ zon khrustalikov.