Interaction of Ni2+ with the tubulin-microtubule system.
In vitro polymerization of purified goat brain tubulin is stimulated at low Ni2+ concentration, whereas at high nickel concentration (greater than 1 . 10(-4)) polymerization is inhibited. Ni2+, over the concentration range of 1 . 10(-4)-1 . 10(-3) M, enhances the rate of colchicine binding to tubulin, although in its presence the colchicine binding site of tubulin is degraded at a much faster rate at 37 degrees C. Finally, incubation of tubulin with Ni2+ at 37 degrees C gradually alters the native conformation of the protein, as revealed by the quenching of intrinsic tryptophan fluorescence.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D006041:Goats; D007700:Kinetics; D046911:Macromolecular Substances; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism*; D009532:Nickel / Q000494:pharmacology*; D014404:Tubulin / Q000378:metabolism*
D046911:Macromolecular Substances; D014404:Tubulin; D009532:Nickel