Assessment of Dharmendra antigen. (V) attempts for purification of specific skin delayed hypersensitivity (DH) inducing antigen (S) from lepromin.
After electrophoresis (in pyrogen-free conditions) of Dharmendra antigen sonicates on agarose gel slides, the components (anionic and cationic) were eluted in pyrogen-free normal saline. On skin testing, it was found that only anionic component produced specific skin DH reactions in tuberculoid leprosy patients. The significance of the observations is discussed.
D000941:Antigens / Q000276:immunology*; D001500:BCG Vaccine / Q000276:immunology; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology* / Q000473:pathology; D007916:Lepromin / Q000276:immunology*; D007918:Leprosy / Q000276:immunology*
D000941:Antigens; D001500:BCG Vaccine; D007916:Lepromin