Stimulatory effect of N-acetyl Muramyl dipeptide in vivo: proliferation of bone marrow progenitor cells in mice.
The effects of single and multiple injections of N-acetyl muramyl dipeptide (MDP) on peripheral leukocytes, colony-forming cells (i.e., bone marrow granulocyte-macrophage progenitor cells), and the humoral immune response (to bovine serum albumin) were investigated in mice. Whereas low doses of MDP (0.1 to 1 mg/kg) provoked lymphocytosis, larger doses (10 mg/kg upward) resulted in lymphocytopenia and an increase in the number of young stab neutrophils and monocytes. MDP induced a dose-dependent increase in the number of bone-marrow macrophage progenitor cells, the maximum being reached by a dose around 10 mg/kg. A 50% increase in the maximum effect was produced by a dose around 0.1 mg/kg. The higher the dose, the longer the increase in these progenitor cells persisted. MDP mediated a dose-dependent antibody response to small amounts of bovine serum albumin, correlating with the proliferation of progenitor cells.
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D001854:Bone Marrow Cells; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D007958:Leukocyte Count; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes; D008218:Lymphocytosis / Q000139:chemically induced; D008231:Lymphopenia / Q000139:chemically induced; D051379:Mice; D008807:Mice, Inbred BALB C; D012710:Serum Albumin, Bovine / Q000276:immunology; D013268:Stimulation, Chemical
D008070:Lipopolysaccharides; D012710:Serum Albumin, Bovine; D000119:Acetylmuramyl-Alanyl-Isoglutamine