Immunochemistry of the cell-surface carbohydrate antigens of Clostridium difficile.
Two carbohydrate cell-surface antigens were extracted from Clostridium difficile. One was extracted from pure cell walls by NaOH and contained glucose, mannose, galactosamine and phosphate in the approximate molar proportions of 2:0.65:1:0.63. The other antigen was extracted with phenol from the disrupted contents of whole cells and purified by chromatography on Sepharose 6B and an immunoabsorbent column; it contained glucose, glucosamine, phosphate and fatty acid in the approximate molar proportions of 2:1:1.6:0.04. Both antigens showed partial immunological identity and both cross-reacted with Clostridium sordellii antiserum. They are analogues of the wall and membrane teichoic acids of other Gram-positive bacteria.
D000942:Antigens, Bacterial / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D003013:Clostridium / Q000276:immunology*; D005227:Fatty Acids / Q000032:analysis; D005688:Galactosamine / Q000032:analysis; D005947:Glucose / Q000032:analysis; D007123:Immunoelectrophoresis, Two-Dimensional; D008358:Mannose / Q000032:analysis; D010710:Phosphates / Q000032:analysis
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D005227:Fatty Acids; D010710:Phosphates; D005688:Galactosamine; D005947:Glucose; D008358:Mannose