Effect of Nocardia rubra cell-wall skeleton on colony-stimulating activity and myeloid colony formation.
The in vivo effect of Nocardia rubra cell-wall skeleton (N-CWS) was studies on the generation of bone marrow granulocyte-macrophage progenitor cells [colony-forming units culture (CFU-c)] and on the levels of myeloid colony-stimulating activity (CSA) in the conditioned medium of the lung (LCM) and serum obtained from mice. In vitro bone marrow culture showed that intraperitoneal (ip) treatment of mice with N-CWS markedly enhanced CFU-c formation induced by stimuli in LCM or serum derived from lipopolysaccharide (LPS)-treated mice. The LCM and serum of mice injected with N-CWS ip were also prepared and assayed for CSA. CSA levels with N-CWS-LCM as well as LPS-LCM used as the standard CSA were markedly greater than those achieved with control LCM. N-CWS-serum also showed a low but significant level of CSA. The CSA level of N-CWS-LCM was maximal at 3 to 5 days after N-CWS injection. These results raise the possibility that enhanced CSA production might be a major mechanism for N-CWS stimulation of granulocyte-macrophage production.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000502:physiology; D002473:Cell Wall; D002478:Cells, Cultured; D006098:Granulocytes / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009615:Nocardia
D008070:Lipopolysaccharides