Proteins of the outer layer of the vitelline membrane of hen's eggs.
A study has been made of the proteins in the vitelline membrane of hen's eggs before and after mechanical separation into the inner and outer layers. The membranes were dissolved in detergent (sodium dodecyl sulphate) and chromatographic fractions were examined by gel electrophoresis. The separated inner and outer layers were compared by gel electrophoresis. The outer layer contained (i) enzymically active lysozyme (EC 3.2.1.17) (about 60% dry weight), (ii) an insoluble ovomucin complex and (iii) a new protein, VMOI (vitelline membrane outer I). These account for most of the protein. In addition, some minor constituents were detected by gel electrophoresis but were not isolated. Except for ovomucin, the constituents of the outer layer could be dissolved from the membrane at high ionic strength (greater than 0.5 M sodium chloride), resulting in a loss of its structure. On lowering the ionic strength the soluble proteins recombined with the membrane, partially regenerating the original structure. Ovomucin appears to form the skeleton of the outer layer, but the salt-soluble proteins, especially lysozyme, are responsible for its integrity. The function of the newly-recognized protein (VMOI) is not known. Its molecular weight is 17,500 according to gel electrophoresis in detergent and it contains no methionine. The inner layer consists largely of the proteins GPI, GPII and GPIII isolated by Kido et al. (Kido, S., Janado, M. and Nunoura, H. (1975) J. Biochem. 78, 261-268) from the whole membrane.
D000818:Animals; D002645:Chickens; D004527:Egg Proteins / Q000302:isolation & purification*; D005260:Female; D008565:Membrane Proteins / Q000302:isolation & purification; D008970:Molecular Weight; D009113:Muramidase / Q000302:isolation & purification; D010059:Ovomucin / Q000302:isolation & purification; D012995:Solubility; D014817:Vitelline Membrane / Q000032:analysis*
D004527:Egg Proteins; D008565:Membrane Proteins; C035651:vitelline membrane proteins; D010059:Ovomucin; D009113:Muramidase