Possible regulatory role of histamine in human platelet function examined by thrombin-induced serotonin release.
The influence of histamine on human platelet function was studied by thrombin-induced serotonin release. The thrombin-induced 3H-serotonin release was confirmed to be a rapid process which does not require external calcium. Histamine was found to reduce the release of serotonin and the inhibition was abolished when H1-plus H2-antagonists were added together with histamine. H1- and H2-receptor stimulation was examined in two ways, by a combination of histamine with cimetidine or diphenhydramine and by the selective agonists 2-(2-pyridyl)-ethylamine and impromidine. In both instances H1- and H2-stimulation was found to reduce the platelet serotonin release. These results suggest a regulatory role of histamine in the platelet function by stimulation of platelet H1- and H2-receptors.
D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D006632:Histamine / Q000097:blood / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D011969:Receptors, Histamine H1 / Q000502:physiology; D011970:Receptors, Histamine H2 / Q000502:physiology; D012701:Serotonin / Q000097:blood*; D013917:Thrombin / Q000494:pharmacology*; D013997:Time Factors
D011969:Receptors, Histamine H1; D011970:Receptors, Histamine H2; D012701:Serotonin; D006632:Histamine; D013917:Thrombin; D002118:Calcium