Differential effect of low molecular weight alcohols on the Con A stimulation of mouse spleen cells.
Incorporation of [3h]thymidine ([3H]TdR) into concanavalin A (Con A)-stimulated murine splenocytes was found to be enhanced by addition of certain concentrations of ethanol, 2-propanol and acetone. The alcohol/acetone-induced enhancement of the Con A response was found to be accompanied by an increase of the percentage of living cells as assessed by trypan blue exclusion. Concentrations of ethanol and 2-propanol which caused maximum [3H]TdR uptake in Con A cultures were also found to lead to higher percentages of aggregated cells than in comparison to Con A cultures without alcohol. The data suggest that alcohols in certain concentrations are capable to achieve optimum Con A stimulation.
D000438:Alcohols / Q000494:pharmacology*; D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002470:Cell Survival; D003208:Concanavalin A / Q000494:pharmacology*; D004306:Dose-Response Relationship, Immunologic; D000431:Ethanol / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011433:Propranolol / Q000494:pharmacology; D013154:Spleen / Q000166:cytology* / Q000276:immunology; D013936:Thymidine / Q000378:metabolism
D000438:Alcohols; D003208:Concanavalin A; D000431:Ethanol; D011433:Propranolol; D013936:Thymidine