Comparison of some peptidic and proteic ovine pineal fractions with a bovine pineal E5 fraction.
Using rather simple and mild extraction and separation methods, three ovine pineal fractions (XM 300 R-PP7.2' and PP7.2 S) were obtained, which contain peptidic/proteic substances and which show fluorescence characteristics of indoles. The ovine fractions were compared with the bovine pineal E-5 fraction. The ovine fractions are chemically sensitive to normal laboratory light and stable in red light (lambda greater than 600 nm). Immunologically, these fractions and the bovine E 5 fraction are stable. From the results of radioimmunological experiments it was concluded that the bovine pineal E 5 fraction as well as the ovine pineal fraction XM 300 R-PP7.2 and PP7.2S may contain (a) peptide(s) ending by the same carboxy terminal tripeptide Pro-Arg-Gly(NH2).
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004589:Electrophoresis, Disc; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010455:Peptides / Q000032:analysis*; D010870:Pineal Gland; D011863:Radioimmunoassay; D012756:Sheep; D013045:Species Specificity; D014020:Tissue Extracts / Q000032:analysis*; D014462:Ultrafiltration
D009419:Nerve Tissue Proteins; D010455:Peptides; D014020:Tissue Extracts