[Synthesis of adenovirus SA7 and Ad5 DNA in extracts from the nuclei of green monkey kidney cells].
A soluble extract from the nuclei of green monkey kidney cells, infected with adenovirus SA7, carries out replication of SA7 DNA. It was observed, that in vitro DNA synthesis, which is dependent on the exogenously added viral DNA-protein complex as its optimal template, proceeds by a semiconservative mechanism. The data obtained are compared with literary data on soluble enzyme systems from adenovirus-infected cells. The early factors provided by SA7 also supported replication of the DNA-protein complex, prepared from human adenovirus type 5, in nuclear extracts of monkey cells.
D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D002474:Cell-Free System; D002522:Chlorocebus aethiops; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics*; D006367:HeLa Cells / Q000382:microbiology; D006801:Humans; D007668:Kidney; D007700:Kinetics; D013045:Species Specificity; D014779:Virus Replication
D004279:DNA, Viral
Sintez DNK adenovirusov SA7 i Ad5 v ékstraktakh iader kletok pochek zelenoĭ martyshki.