Region-specific patterns of mucin reaction demonstrated by paradoxical concanavalin A-staining in normal colonic epithelium and in colorectal cancers induced in rats by 1,2-dimethylhydrazine.
The histochemistry of mucin in normal large intestine and in experimental colorectal cancers induced in Wistar rats with 1,2-dimethylhydrazine was analyzed by modifications of the concanavalin A-horseradish peroxidase method (paradoxical concanavalin A-staining) and high-iron diamine-Alcian blue (pH 2.5) staining (HID-AB). Quantitative analysis of each mucin reaction was done by the use of an image analyzer. Regional variations in the percentage area of "labile class III mucin" were 1 to 50% in the distal colon and 50 to 90% in the proximal colon. Regional variations in the percentage area of sulfated sialomucin were 80 approximately 100% in the distal colon and under 20% in the proximal colon. The percentage area of "labile class III mucin" in primary colorectal cancers showed region-specific variation and that in metastatic colorectal cancers also showed similar region-specific variation of colorectal mucosa where primary tumors were located. In contrast, there were no region-specific mucin reactions for sulfated sialomucin in almost all primary and metastatic colorectal cancers.
D019813:1,2-Dimethylhydrazine; D000818:Animals; D003106:Colon / Q000032:analysis*; D003110:Colonic Neoplasms / Q000032:analysis* / Q000139:chemically induced; D003208:Concanavalin A; D004127:Dimethylhydrazines; D004848:Epithelium / Q000032:analysis; D006651:Histocytochemistry; D007413:Intestinal Mucosa / Q000032:analysis*; D008297:Male; D009077:Mucins / Q000032:analysis*; D009374:Neoplasms, Experimental / Q000032:analysis / Q000139:chemically induced; D051381:Rats; D011919:Rats, Inbred Strains; D012004:Rectal Neoplasms / Q000032:analysis* / Q000139:chemically induced
D004127:Dimethylhydrazines; D009077:Mucins; D003208:Concanavalin A; D019813:1,2-Dimethylhydrazine