[Induction of chromosome aberrations and mitotic disorders in a bone marrow cell culture of Papio hamadryas in exposure to cytochalasin B].
A 24-48 hours' treatment with cytocholasin B (1 mg/ml) increases significantly the number of chromosomal aberrations and mitotic abnormalities in primary cultures of bone marrow of the baboon Papio hamadrias. The addition of erythropoietin in concentration of 0.25 units per ml, either before or after the addition of cytochalasin, increases the number of chromosomal aberrations due to hormone stimulation of cell division. At this concentration, erythropoietin alone does not induce any chromosomal aberrations, but stimulated mitotic activity. Cytochalasin B induced aberrations in chromosomes increase the frequency of chromosome rearrangements of the chromatid type, while the main type of mitotic aberrations observed was the induction of multinuclear aneuploid cells.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects* / Q000648:ultrastructure; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003571:Cytochalasin B / Q000494:pharmacology*; D004921:Erythropoietin / Q000494:pharmacology; D008938:Mitosis / Q000187:drug effects*; D010215:Papio
D004921:Erythropoietin; D003571:Cytochalasin B
Induktsiia aberratsiĭ khromosom i mitoticheskikh narusheniĭ v kul'ture kletok kostnogo mozga paviana gamadrila pri deĭstvii tsitokhalazina B.