Increase in numbers of 6-thioguanine-resistant human lymphocytes in short-term culture.
Lymphocytes from healthy young adults and from adolescent patients with cancer were examined for their ability to incorporate [3H]thymidine in short-term culture in the presence of phytohemagglutinin and 6-thioguanine (6-TG). The numbers of labeled nuclei after 72 h in culture were compared to numbers of labeled nuclei after 30 h in culture. The numbers of labeled nuclei in the presence of 6-TG increased 6-65-fold between 30 and 72 h. The increases in number could be accounted for by 3-6 cycles of cell division. The data suggest that 6-TG-resistant peripheral blood lymphocytes are capable of reproduction in short-term culture, but factors other than reproduction may also contribute to increases in numbers of 6-TG-resistant cells.
D000328:Adult; D002478:Cells, Cultured; D004351:Drug Resistance; D006689:Hodgkin Disease / Q000503:physiopathology*; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects* / Q000502:physiology; D008940:Mitotic Index; D012016:Reference Values; D012208:Rhabdomyosarcoma / Q000503:physiopathology*; D012512:Sarcoma, Ewing / Q000503:physiopathology*; D013866:Thioguanine / Q000494:pharmacology*
D013866:Thioguanine