Determination of p-hydroxyphenylacetic acid in cerebrospinal fluid by high-performance liquid chromatography with electrochemical detection.
Using reversed-phase high-performance liquid chromatography and electrochemical detection with a rapid one-step purification on a Sephadex G-10 column, we have developed a sensitive technique to measure p-hydroxyphenylacetic acid, the oxidatively deaminated metabolite of p-tyramine. The metabolite was measured in cerebrospinal fluid with high recovery and precision. Concentrations of p-hydroxyphenylacetic acid ranged from 4.2 to 17.0 ng/ml with a mean value of 7.8 ng/ml +/- 1.1 SEM in lumbar cerebrospinal fluid of 11 control patients. In a preliminary analysis, the concentrations in schizophrenic patients were significantly lower than those in controls. Since p-hydroxyphenylacetic acid in cerebrospinal fluid seems to be derived from the brain, this finding may indicate a decrease in brain formation. The present method should be useful in clinical investigations to clarify a functional role of p-tyramine in the brain.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000134:cerebrospinal fluid; D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006719:Homovanillic Acid / Q000134:cerebrospinal fluid; D006801:Humans; D007223:Infant; D008722:Methods; D008875:Middle Aged; D010648:Phenylacetates / Q000134:cerebrospinal fluid*; D012559:Schizophrenia / Q000134:cerebrospinal fluid
D010648:Phenylacetates; D015102:3,4-Dihydroxyphenylacetic Acid; C008070:4-hydroxyphenylacetic acid; D006719:Homovanillic Acid