Studies on inherited and acquired metabolic disorders in cultured arterial smooth muscle cells and fibroblasts.
Cultured mesenchymal cells from insulin- and non-insulin-dependent diabetics with poor long-term control showed an increased growth rate as compared to age matched normal cells independent of the serum type used. These growth changes occurred independent of the genetic background as mesenchymal cells, (a) from four pancreatectomized diabetics with poor long-term metabolic control showed similar increased growth rates and (b) from two diabetics with good metabolic long-term control showed no growth changes as compared to age matched controls. There is no evidence (a) for the specific impact of obesity on these cell associated growth changes, and (b) for cell associated differences between cultured mesenchymal cells from diabetics and normal controls as far as protein and lipoprotein metabolism are concerned. The metabolic control of diabetes is of primary importance for the regulation of growth, protein synthesis and for lipoprotein binding and degradation in cultured mesenchymal cells independent of their origin, as serum from diabetics with poor metabolic control increased in these cells growth, protein synthesis and lipoprotein metabolism. Serum from well controlled diabetics did not differ from normal serum in its growth stimulating effects. As far as obesity could effect the metabolic control of diabetes, probably this could also affect indirectly the metabolism of mesenchymal cells. The interaction of cell associated and serum factors can further increase the observed growth and metabolic changes of mesenchymal cells. As far as these cell and serum related changes also occur in vivo they could contribute to the accelerated atherogenic development in diabetics as well as to an increased basal lamina layering in different tissues.
D000293:Adolescent; D000328:Adult; D002455:Cell Division; D002478:Cells, Cultured; D002648:Child; D003920:Diabetes Mellitus / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007930:Leucine / Q000378:metabolism; D008074:Lipoproteins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D010180:Pancreatectomy; D011392:Proline / Q000378:metabolism
D008074:Lipoproteins; D011392:Proline; D007930:Leucine