[Analysis of the fragment D-fibrin monomer complex formation by salting-out fractionation].
The fragment D-fibrin monomer complex formation was studied by ammonium sulfate fractionation of the reaction mixtures initially consisting of 0.15-4.00 mg/ml of fragment D and 1.00 mg/ml of fibrin monomer, It was shown that in these mixtures the equilibrium was reached in not more than 15 sec. Fragment D binding to fibrin dependent on the fragment concentration. The Scatchard analysis of this dependence revealed a maximum of fragment D binding to one fibrin molecule of 2.95 +/- 0.28 with a Ka value of (9.04 +/- 1.08) x 10(-5) M-1 (n = 6), thus indicating a strong fragment--fibrin affinity.
D000645:Ammonium Sulfate; D005337:Fibrin / Q000302:isolation & purification* / Q000378:metabolism; D005338:Fibrin Fibrinogen Degradation Products / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D046911:Macromolecular Substances; D009994:Osmolar Concentration; D011485:Protein Binding
D005338:Fibrin Fibrinogen Degradation Products; D046911:Macromolecular Substances; C015214:fibrinogen D fragment; D005337:Fibrin; D000645:Ammonium Sulfate
Issledovanie kompleksoobrazovaniia fragmenta D s monomernym fibrinom metodom fraktsionnogo vysalivaniia.