Selenium-dependent growth and glycine fermentation by Clostridium purinolyticum.
Clostridium purinolyticum fermented glycine as a sole carbon and energy source according to the equation: 4 Glycine + 2H2O leads to 3 Acetate + 2CO2 + 4NH3. The organism required adenine as a supplement and selenium compounds as micronutrients for growth. The molar growth yield on glycine was 6.5 g dry wt. Radiochemical and enzymic investigations revealed a new fermentation pathway for glycine in which 1 mol glycine was completely oxidized to CO2 and the generated reducing equivalents were used to reduce a further 3 mol glycine to acetate via the glycine reductase system. This reaction was associated with the formation of ATP.
D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D003013:Clostridium / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D005998:Glycine / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism; D012643:Selenium / Q000378:metabolism*
D009097:Multienzyme Complexes; D000594:Amino Acid Oxidoreductases; C020815:glycine reductase; D012643:Selenium; D005998:Glycine