Preparation of gels from hyaluronate solutions.
Stable, transparent gels can be prepared from hyaluronate solutions by adding CuSO4. The best gelation was found using solutions of 2 mg/ml hyaluronate in glass-distilled water at a pH of 6.2 after the addition of 1 mg/ml CuSO4. Methylation of the carboxyl groups of the hyaluronate completely abolished the gelation, indicating the importance of the carboxyl groups for the gel formation with Cu2+ ions. Gelation also depends on the molecular size of the hyaluronate, since hyaluronate was not able to form a gel after depolymerization with hyaluronidase.
D000818:Animals; D002417:Cattle; D004339:Drug Compounding; D005782:Gels; D006820:Hyaluronic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009883:Ophthalmic Solutions; D014822:Vitreous Body / Q000378:metabolism
D005782:Gels; D009883:Ophthalmic Solutions; C036036:hyaluronic acid methyl ester; D006820:Hyaluronic Acid