Peroxidase and IAA oxidase in germinating seeds of Cicer arietinum L.
Peroxidase and indole acetic oxidase (IA oxidase) activities in seeds of Cicer arietinum L. were studied at 72 h of germination. Both activities behaved differently in relation to optimum pH, stability against temperature and time of storage of 4 degrees C. Disc electrophoresis on polyacrilamide gel showed the existence of five anionic and three cationic isoenzymes with peroxidase activity by using benzidine as substrate; three of the anionic isoenzymes and one cationic isoenzyme possessed a simultaneous IAA oxidase activity as well.
D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D007887:Fabaceae / Q000201:enzymology*; D007210:Indoleacetic Acids / Q000378:metabolism; D007527:Isoenzymes / Q000302:isolation & purification*; D010544:Peroxidases / Q000302:isolation & purification*; D010946:Plants, Medicinal; D012639:Seeds / Q000201:enzymology*
D007210:Indoleacetic Acids; D007527:Isoenzymes; C027929:indoleacetic acid oxidase; D010544:Peroxidases