Effects of 20,25-diazacholesterol on cholesterol synthesis in cultured chick muscle cells: a radiogas chromatographic and mass spectrometric study of the post-squalene sector.
Radiogas chromatography, used in conjunction with mass spectrometry, has been used to analyze the sterol content of cultured chick muscle cells. Seven sterols, plus lanosterol, were detected. These sterols conformed to a linear biosynthetic pathway linking lanosterol and cholesterol. The reaction sequence is: C-14 demethylation, C-4 demethylation, delta 8 leads to delta 5 double bond rearrangement, delta 24 double bond reduction. When chick cells were treated with increasing concentrations of 20,25-diazacholesterol, components of this pathway and aberrant products accumulated. These accumulations suggest that diazacholesterol affects reductases, double bond isomerases and the C-14 demethylation enzymes of sterol biosynthesis.
D000818:Animals; D001373:Azacosterol / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000096:biosynthesis*; D003897:Desmosterol / Q000096:biosynthesis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007810:Lanosterol / Q000378:metabolism; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D013185:Squalene / Q000378:metabolism
D007810:Lanosterol; D003897:Desmosterol; D013185:Squalene; D002784:Cholesterol; D001373:Azacosterol