[Use of affinity immunoelectrophoresis in the presence of concanavalin A for characterization of neurospecific protein D2].
The affinity immunoelectrophoresis method in the presence of ConA was used to determine the dissociation constant of the human neurospecific protein D2 complex with lectin. Preliminarily protein D2 from the triton extract of the human embryo brain membrane fraction was partly purified by chromatography on phenyl-sepharose and was identified by the method of rocket-line immunoelectrophoresis with antiserum against neurospecific antigen D2 of the rat brain.
D001921:Brain / Q000196:embryology; D001923:Brain Chemistry; D002462:Cell Membrane / Q000032:analysis; D003208:Concanavalin A; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D011247:Pregnancy
D009419:Nerve Tissue Proteins; C488879:brain-specific protein D2, human; D003208:Concanavalin A
Ispol'zovanie affinnogo immunoélektroforeza v prisutstvii konkanavalina A dlia kharakteristiki neĭrospetsificheskogo belka D2.