[In vitro determination of the biological activity of choleragen by the anaerobic dehydrogenase inhibition test in a surviving blastoma cell culture].
A rapid method for the detection and titration of choleragen in vitro in the test allowing one to evaluate dehydrogenase activity in the surviving blastoma culture has been developed. In comparison with the commonly accepted method of using the ligated loop in adult rabbits, the above method is simple, highly sensitive and economical.
D000693:Anaerobiosis / Q000187:drug effects*; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000633:toxicity*; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007422:Intestines / Q000187:drug effects; D008223:Lymphoma / Q000201:enzymology*; D008660:Metabolism / Q000187:drug effects*; D051379:Mice; D009374:Neoplasms, Experimental / Q000201:enzymology; D010088:Oxidoreductases / Q000037:antagonists & inhibitors*; D011817:Rabbits
D002772:Cholera Toxin; D010088:Oxidoreductases
Opredelenie biologicheskoĭ aktivnosti kholerogena in vitro po testu podavleniia anaérobnykh degidrogenaz perezhivaiushcheĭ kul'tury blastomnykh kletok.