The accurate determination of serum glucose by isotope dilution mass spectrometry--two methods.
Two isotope dilution mass spectrometric methods have been developed for the determination of D-glucose in human serum. Each uses a uniformly labeled (13C)glucose as the internal standard. The first method involves conversion of glucose into 1,2:5,6-di-O-isopropylidene-alpha-D-glucofuranose and an extensive clean-up, followed by quantitation using packed column gas chromatography mass spectrometry. In the second method, glucose is converted into alpha-D-glucofuranose cyclic 1,2:3,5-bis(butylboronate)-6-acetate. The wet chemistry work-up is simpler, but analysis by capillary gas chromatography mass spectrometry is required. Both methods exhibit excellent precision (coefficients of variation less than 0.3%) and provided mean values that agree within 1% for all serum pools tested.
D001786:Blood Glucose / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000031:analogs & derivatives; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D012015:Reference Standards
D001786:Blood Glucose; C036124:1,2-5,6-di-O-isopropylidene-D-glucofuranose; C036123:D-glucofuranose cyclic 1,2-3,5 bis(butylboronate)-6-acetate; D005947:Glucose