Extraction and partial purification of a CEA-like BCA from a pool of pre-selected breast carcinomas.
By exploiting the cross-reaction among the antigens associated with a pool of breast carcinomas, preselected according to their antigenic content, a CEA-like BCA, with a specific activity of 1400 U/micrograms has been extracted, by means of 3M KCl, and partially purified by means of ConA Sepharose affinity chromatography and bioabsorption, employing, for the latter, a cross-reacting antiserum (anti CEA). The subsequent labelling, after Sephadex G-200 gel filtration, points out an immunological activity at the apex of the first peak versus anti CBC (or C.BCA) and anti CEA.
D000042:Absorption; D000818:Animals; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D001943:Breast Neoplasms / Q000032:analysis*; D002272:Carcinoembryonic Antigen / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D003429:Cross Reactions; D005260:Female; D006041:Goats / Q000276:immunology; D006801:Humans; D011189:Potassium Chloride
D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; D011189:Potassium Chloride