Satellite DNA of Drosophila nasuta nasuta and D. n. albomicana: localization in polytene and metaphase chromosomes.
The DNA from the two Drosophila nasuta races, D. n. nasuta and D. n. albomicana was investigated by CsCl density gradient centrifugation. D. n. nasuta has one major AT-rich satellite DNA sequence with a density of 1.664 g/cm3, while D. n. albomicana has at least three satellites with densities of 1.674 g/cm3, 1.665 g/cm3 and 1.661 g/cm3. The isolated satellite sequences hybridize in situ to all heterochromatic regions of all metaphase chromosomes of both races. In polytene chromosomes the satellite sequences hybridize exclusively to the chromocenter. All chromosomal regions hybridizing with the satellites show also bright quinacrine fluorescence.
D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D002875:Chromosomes / Q000502:physiology*; D003433:Crosses, Genetic; D004276:DNA, Satellite / Q000235:genetics* / Q000302:isolation & purification; D004330:Drosophila / Q000235:genetics*; D005260:Female; D007814:Larva; D008297:Male; D008677:Metaphase; D009693:Nucleic Acid Hybridization; D013045:Species Specificity; D013050:Spectrometry, Fluorescence
D004276:DNA, Satellite