An XAD-2 resin method for efficient extraction of mutagens from fried ground beef.
4 methods of extraction of mutagens from fried ground beef were compared for total mutagen recovery and chromatographic profile of isolated substances. A method which employs Amberlite XAD-2 resin to isolate mutagenic activity from an initial aqueous acid extract of fried beef was found to yield approx. 4 times more activity than other aqueous or organic solvent extraction procedures. Chromatographic profiles of mutagenic extracts isolated by the 4 methods suggest that the XAD-2 resin method does not recover different mutagens, but is primarily a more efficient isolation procedure. The resin method is rapid, inexpensive, simple, and requires approx. half the time of the other methods.
D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000379:methods; D003296:Cooking; D007475:Ion Exchange Resins; D008460:Meat / Q000032:analysis*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000302:isolation & purification*; D009154:Mutation; D011137:Polystyrenes
D007475:Ion Exchange Resins; D009153:Mutagens; D011137:Polystyrenes; C003771:Amberlite XAD-2 resin