Effect of the tumor promotor 12-O-tetradecanoylphorbol-13-acetate on the rate of disappearance of nicotine from mouse skin.
The rate of disappearance of nicotine from mouse skin was increased by the presence of 12-O-tetradecanoylphorbol-13-acetate (TPA) during the first 2-hour period following the application of nicotine to mouse skin. The biological half-life (t1/2) for nicotine alone was 1.2-1.3 h whereas in the presence of TPA, t1/2 was 0.8 to 0.9 h. The disappearance of nicotine from mouse skin followed first-order kinetics over a period of 6 h whereas the loss of nicotine in the presence of TPA from mouse skin followed first-order kinetics for the first 2 h, then the nicotine level became fairly constant for the time period of 2-5 h. Benzo (alpha) pyrene did not affect the rate of disappearance of nicotine from mouse skin. Neither cotinine nor nicotine 1'-N oxide, metabolites of nicotine, was detected in mouse skin following the topical application of this alkaloid.
D000287:Administration, Topical; D000818:Animals; D001580:Benzopyrenes / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D006207:Half-Life; D007700:Kinetics; D051379:Mice; D008813:Mice, Inbred ICR; D009538:Nicotine / Q000008:administration & dosage / Q000378:metabolism*; D010704:Phorbols / Q000494:pharmacology*; D012867:Skin / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000008:administration & dosage / Q000494:pharmacology*
D001580:Benzopyrenes; D010704:Phorbols; D009538:Nicotine; D013755:Tetradecanoylphorbol Acetate