Isolation and native characterization of cysteine-rich collagens from bovine placental tissues and uterus and their relationship to types IV and V collagens.
A simplified procedure for the fractionation and purification of different collagen types from various tissues is described which is particularly efficient in separating type-V from type-IV collagen, and high-mol.-wt. (HMW) aggregates from 7 S collagen. Uterus and maternal villi contain 2 forms of type-V collagen - [alpha 1(V)]2 alpha 2(V) and [alpha 1(V) alpha 2(V) alpha 3(V) - which have been separated on DEAE-cellulose. Uterus however appears to be the richest source  of both HMW aggregates and the [alpha 1(V) alpha 2(V) alpha 3(V)] collagen, and a probable relationship between these collagens is discussed.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D003094:Collagen / Q000032:analysis* / Q000145:classification; D003545:Cysteine / Q000032:analysis; D005260:Female; D008970:Molecular Weight; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy; D014599:Uterus / Q000032:analysis*
D000596:Amino Acids; D003094:Collagen; D003545:Cysteine