Labelling of dying neurones by peroxidase injected intravascularly in chick embryos.
Three lines of evidence indicate that intravascularly injected horseradish peroxidase labels dying neurones selectively in the developing isthmo-optic nucleus: (i) in unoperated embryos, labelled neurones are found only during the period of normal neuronal death; (ii) some of the labelled neurones are manifestly necrotic; (iii) an intraocular injection of colchicine causes essentially all the isthmo-optic neurones to degenerate, and then they almost all take up peroxidase. However, deafferenting the developing isthmo-optic nucleus by a tectal lesion increases the loss of neurones whilst scarcely affecting the number labelled; this implies that the neurones which die because of the deafferentation either do not take up the peroxidase or are phagocytozed exceptionally rapidly.
D000818:Animals; D001921:Brain / Q000196:embryology / Q000473:pathology*; D002642:Chick Embryo; D006735:Horseradish Peroxidase; D007700:Kinetics; D009416:Nerve Regeneration; D009474:Neurons / Q000502:physiology*; D013477:Superior Colliculi / Q000502:physiology*
D006735:Horseradish Peroxidase