Stimulation by phytohaemagglutinin of peripheral blood lymphocytes from horse, pig, sheep and man.
Optimal conditions for stimulation by phytohaemagglutinin (PHA) were established for equine, porcine, ovine and human lymphocytes in MEMS medium. Optimal thymidine concentration was determined for assay of cell transformation. With all species tested horse serum gave highest thymidine incorporation. Homologous serum was not more appropriate for lymphocytes of man, pig and sheep. Optimal stimulation was achieved at 20, 0.5-5, 5, and 10-40 micrograms PHA per 10(6) cells for human, equine, porcine and ovine lymphocytes, respectively.
D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006736:Horses / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008934:Mitogens; D010835:Phytohemagglutinins / Q000494:pharmacology*; D012756:Sheep / Q000276:immunology; D013045:Species Specificity; D013552:Swine / Q000276:immunology; D013936:Thymidine / Q000378:metabolism
D008934:Mitogens; D010835:Phytohemagglutinins; D013936:Thymidine