On-line direct liquid introduction interface for micro-liquid chromatography/mass spectrometry: application to drug analysis.
We describe an integrated micro-liquid chromatograph/mass spectrometer (micro-LC/MS) system capable of performing routine determinations for 1--10 ng of drugs and their metabolites extracted from biological fluids. The micro-LC is constructed from conventional "high-performance" liquid-chromatographic instrumentation by using commercially available components. The mass spectrometer is operated in the chemical ionization mode. The direct liquid introduction micro-LC/MS interface can be constructed from commercially available materials. Chromatographic and mass spectral results demonstrate the ability of the micro-LC and micro-LC/MS system to separate and determine multiple components in standards of trace concentrations and in equine urinary extracts. The stability and sensitivity of this micro-LC/MS system are demonstrated through determinations of trichlormethiazide.
D000818:Animals; D002740:Chlorothiazide / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D004300:Doping in Sports; D006736:Horses; D006852:Hydrochlorothiazide / Q000652:urine; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods; D014237:Trichlormethiazide / Q000652:urine*
D006852:Hydrochlorothiazide; D002740:Chlorothiazide; D014237:Trichlormethiazide