Analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography.
A standard mixture of 25 short-chain fatty acids was resolved by high-performance liquid chromatography, using an Aminex HPX-87 column. The acids produced in culture media by anaerobic bacteria were analyzed by high-performance liquid chromatography after extraction with ether and reextraction into a small volume of 0.1 N NaOH. The presence of fumaric acid in culture extracts of Peptostreptococcus anaerobius was confirmed by gas chromatography-mass spectrometry analysis of the trapped eluent fractions from the high-performance liquid chromatography column.
D001441:Bacteroides fragilis / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003013:Clostridium / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis*; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005232:Fatty Acids, Volatile / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D010462:Peptostreptococcus / Q000032:analysis*
D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D005232:Fatty Acids, Volatile