[Increased lysosomal membrane permeability in hexachlorobenzene poisoning in rabbits].
To assess the effect of the fungicide hexachlorobenzene (HCB) on lysosomal membrane stability in rabbits, the authors determined the activity of two lysosomal enzymes (beta-N-acetylglucosaminidase, CP 3.2.1.30 and alpha-mannosidase, CP 3.2.1.24) in the blood serum, and free unsedimentable activity of the former enzyme in liver homogenates. The activities of beta-N-acetylglucosaminidase and alpha-mannosidase were found to be enhanced on the 7th and 50th days after daily administration of HCB in doses 280 and 105 mumol/kg body mass, respectively. In chronic experiments, there was also an increase in free and unsedimentable activity of beta-N-acetylglucosaminidase in liver homogenates. Increased permeability of lysosomal membranes due to HCB indicates that lysosomes and acid hydrolases are implicated in the pathogenesis of intoxication under consideration.
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002722:Chlorobenzenes / Q000506:poisoning*; D006581:Hexachlorobenzene / Q000506:poisoning*; D007425:Intracellular Membranes / Q000187:drug effects*; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D011817:Rabbits; D013997:Time Factors
D002722:Chlorobenzenes; D006581:Hexachlorobenzene
Povyshenie pronitsaemosti lizosomal'nykh membran pri intoksikatsii geksakhlorbenzenom u krolikov.