A quantitative autoradiographic method for the measurement of local rates of brain protein synthesis.
We have developed a new method for measuring local rates of brain protein synthesis in vivo. It combines the intraperitoneal injection of a large dose of low specific activity amino acid with quantitative autoradiography. This method has several advantages: 1) It is ideally suited for young or small animals or where immobilizing an animal is undesirable. 2 The amino acid injection "floods" amino acid pools so that errors in estimating precursor specific activity, which is especially important in pathological conditions, are minimized. 3) The method provides for the use of a radioautographic internal standard in which valine incorporation is measured directly. Internal standards from experimental animals correct for tissue protein content and self-absorption of radiation in tissue sections which could vary under experimental conditions.
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D002250:Carbon Radioisotopes; D007700:Kinetics; D008722:Methods; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D014633:Valine / Q000378:metabolism
D002250:Carbon Radioisotopes; D009419:Nerve Tissue Proteins; D014633:Valine