A convenient and rapid method for the complete removal of CoA from butyryl-CoA dehydrogenase.
The commercially available gel, 2-pyridyl disulphide hydroxypropyl ether-Sepharose (thiopropyl-Sepharose 6B), can be used to remove bound ligand completely from butyryl-CoA dehydrogenase (EC 1.399.2) in two simple operations. The resultant enzyme forms normal complexes with acetoacetyl-CoA and CoA persulphide, contains no bound CoA as determined by the enzymatic assay for CoA, and retains full catalytic activity.
D000818:Animals; D044926:Butyryl-CoA Dehydrogenase; D003065:Coenzyme A / Q000302:isolation & purification*; D044943:Fatty Acid Desaturases / Q000302:isolation & purification*; D006089:Gram-Negative Anaerobic Bacteria / Q000201:enzymology; D008024:Ligands; D011485:Protein Binding; D012685:Sepharose / Q000031:analogs & derivatives
D008024:Ligands; C035872:thiopropyl-sepharose; D012685:Sepharose; D044943:Fatty Acid Desaturases; D044926:Butyryl-CoA Dehydrogenase; D003065:Coenzyme A