A method of exocrine pancreatectomy in the rat.
The abdominal cavity of male albino rats, average weight 250 g, was opened and the duodenum approached where the ductus choledochus joins. Pancreatic ducts empty into the lower third of the choledochus. The ductus choledochus was cannulated with a Teflon catheter beneath the liver and its other end was brought out at the neck of the rat. Tissue glue was introduced into the lower part of the choledochus towards the liver. Another Teflon catheter was introduced into the duodenum, its second end brought out at the neck and connected with the first catheter by means of a metal tube, thus preserving bile flow from the liver to the duodenum. Tissue glue injection results in complete atrophy of the exocrine pancreas within 2-15 days. The islets of Langerhans remained intact.
D000818:Animals; D002404:Catheterization / Q000379:methods; D003135:Common Bile Duct / Q000601:surgery; D008297:Male; D010179:Pancreas / Q000033:anatomy & histology; D010180:Pancreatectomy / Q000379:methods*; D010183:Pancreatic Ducts / Q000601:surgery; D051381:Rats; D011919:Rats, Inbred Strains; D014014:Tissue Adhesives / Q000008:administration & dosage*
D014014:Tissue Adhesives