Formation of granulocytes and macrophages in mouse bone marrow cultures exposed to various anaesthetics.
The effects of anaesthetics on mouse bone marrow colony growth in vitro were examined. The culture dishes were kept in boxes of stainless steel, so that the composition of the gas phase could easily be controlled. After 1 week of culturing, cell colonies were counted. The cells (macrophages and in one type of culture also granulocytes) were then washed out of the dishes and counted. Enflurane, as well as halothane, present in the gas phase at concentrations used clinically, decreased the number of colonies and cells in a dose-dependent fashion. However, intravenously administered drugs such as diazepam, fentanyl, alfentanyl, sufentanyl, thiopental and pentobarbital were not inhibitory at concentrations used in anaesthetic practice, but at least some of them depressed cell formation when high concentrations were used.
D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D001854:Bone Marrow Cells; D003114:Colony-Forming Units Assay; D003975:Diazepam / Q000494:pharmacology; D004737:Enflurane / Q000494:pharmacology; D005260:Female; D006098:Granulocytes / Q000187:drug effects*; D006221:Halothane / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects*; D051379:Mice
D000777:Anesthetics; D004737:Enflurane; D003975:Diazepam; D006221:Halothane