Quantitative immunoassays for diagnosis and carrier detection in cystic fibrosis.
Quantitative immunoprecipitation and immunoradiometric assays have been developed for a protein present in the serum of cystic fibrosis homozygotes, and to a lesser extent in the serum of heterozygotes. When tested on a panel of sera from 14 cystic fibrosis patients, 29 heterozygotes and 23 controls, the immunoprecipitation assay allowed correct assignments to be made on 94% of occasions with one batch of antiserum and 95% with another. With the same panel of sera, the immunoradiometric assay allowed 94% correct assignments. It is suggested that such accuracy is the maximum that can be expected in the present state of knowledge of cystic fibrosis.
D001798:Blood Proteins / Q000235:genetics*; D003550:Cystic Fibrosis / Q000175:diagnosis* / Q000235:genetics; D006580:Genetic Carrier Screening; D006720:Homozygote; D006801:Humans; D007122:Immunoelectrophoresis; D011863:Radioimmunoassay; D012016:Reference Values
D001798:Blood Proteins