Penetration and localization of furocoumarins in single living cells studied by microspectrofluorometry.
The microspectrofluorometric technique has been used to study the penetration and the localization of psoralen, 4,5',8-trimethylpsoralen and 4'-aminomethyltrioxsalen in single living L-cells. The concentration of the different compounds inside the cell reached a plateau in 2 min with psoralen and aminomethyltrioxsalen and in 20 min with trioxsalen. Washing of the cells with culture medium produced only a partial removal of the three furocoumarins, distributed apparently in equivalent amount in the nucleus and cytoplasm.
D000818:Animals; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000378:metabolism; D011564:Furocoumarins / Q000378:metabolism*; D007739:L Cells / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D013329:Structure-Activity Relationship; D014307:Trioxsalen / Q000031:analogs & derivatives / Q000378:metabolism
D003432:Cross-Linking Reagents; D011564:Furocoumarins; C018822:aminomethyltrioxsalen; D014307:Trioxsalen