Belladonna alkaloids and phenobarbital combination pharmaceuticals analysis I: High-performance liquid chromatographic determinations of hyoscyamine-atropine and scopolamine.
High-performance liquid chromatographic separations are described for the analysis of hyoscyamine-atropine and scopolamine in combination pharmaceutical dosage forms containing phenobarbital. A mobile phase containing 0.034 M tetramethylammonium phosphate in water-methanol (21:10, pH 2.0) separated hyoscyamine or atropine from scopolamine on an octadecylsilane column in less than 9 min. Monitoring of the column effluent at 220 nm gave a detection limit of 0.02 microgram for each alkaloid. Hyoscyamine sulfate and/or atropine sulfate were determined as total equivalent hyoscyamine sulfate, and scopolamine hydrobromide was determined as a separate entity. Data from the application of the method to commercial pharmaceutical products are also presented.
D001285:Atropine / Q000032:analysis*; D002214:Capsules / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004355:Drug Stability; D010634:Phenobarbital / Q000032:analysis*; D012601:Scopolamine / Q000032:analysis*; D012996:Solutions / Q000032:analysis; D013607:Tablets / Q000032:analysis
D002214:Capsules; D004338:Drug Combinations; D012996:Solutions; D013607:Tablets; D001285:Atropine; D012601:Scopolamine; D010634:Phenobarbital