The action of aldosterone and ouabain on cation concentrations in incubated renal slices.
Studies have been made on the effects of aldosterone and ouabain on Na+ and K+ concentrations in incubated rat kidney slices. The results indicate that, control slices gained Na+ over the first 10 min of incubation and subsequently extruded it, with increase in incubation time. In the presence of aldosterone, the same pattern was observed as with control slices, but tissue Na+ concentrations were significantly higher at all times. In the presence of ouabain, slices accumulated Na+ continuously, but in the presence of both drugs, tissue Na+ concentrations were not as high as with ouabain alone. Both aldosterone and ouabain increased the loss of cell K+ whereas control slices reaccumulated their intracellular K+ concentration, with increase in incubation time. The results suggest that aldosterone may have an actual mechanism of action in the renal tubule: either by activating two Na+, K+ pumps located on the luminal and serosal membranes, respectively, or by enhancing luminal membrane permeability, and at the same time stimulating Na+, K+-ATPase on the serosal membrane.
D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D002412:Cations / Q000378:metabolism*; D005110:Extracellular Space / Q000187:drug effects; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism; D011887:Raffinose; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism
D002412:Cations; D000450:Aldosterone; D010042:Ouabain; D012964:Sodium; D011887:Raffinose; D011188:Potassium