Autofluorescence as an index of ageing in human fibroblasts in culture.
An exponential increase in autofluorescence (AF) during serial passaging of human diploid fibroblasts, strain MRC-5, was observed using a fluorescence-activated cell sorter. Skin fibroblasts from patients with premature-ageing syndromes had high levels of AF, whereas virus-transformed cell lines had negligible amounts of AF.
D000328:Adult; D000368:Aged; D000375:Aging; D002460:Cell Line; D004622:Embryo, Mammalian; D005260:Female; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D008168:Lung / Q000502:physiology*; D008856:Microscopy, Fluorescence; D011247:Pregnancy; D012867:Skin / Q000254:growth & development* / Q000503:physiopathology; D014898:Werner Syndrome / Q000503:physiopathology*