[Alanine transport in a mammalian cell culture].
The transport of L-alanine in cultured 3T3, 3T6 and CHO cells has been studied. The estimated Michaelis kinetic constants KM and Vmax for 3T3, 3T6 and CHO cells were 0.6 mM and 3 mM/min, 2.5 mM and 8.3. mM/min, 0.7 mM and 8.4 mM/min, respectively. Alanine transport was inhibited by glycine, serine, alpha-aminoisobutyric acid, methionine, and in a lesser degree by leucine and valine. Glycine depressed alanine transport with the constant of inhibition (Ki) similar to the constant of glycine transport (Km). Alanine transport was strictly diminished in a Na+-dependent medium at the expense of the increase of Km for transport. It is concluded that in 3T3, 3T6 and CHO cells, alanine is transported by the Na+-dependent "A" system.
D000409:Alanine / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D003864:Depression, Chemical; D005347:Fibroblasts / Q000378:metabolism*; D006168:Guinea Pigs; D007700:Kinetics; D051379:Mice; D012964:Sodium / Q000378:metabolism
D012964:Sodium; D000409:Alanine
Transport alanina v kul'ture kletok mlekopitaiushchikh.