Glutathione-S-transferase and GSH-peroxidase activities during the state of GSH-depletion leading to lipid peroxidation in rat liver.
Depletion of hepatic glutathione (GSH) in phenobarbital-induced rats by phorone (diisopropylidene acetone; 0.25 g/kg i.p.) or vinylidene chloride (VDC; 0.5 g/kg i.p.) led to an enhanced lipid peroxidation in vitro as evidenced by the measurements of malondialdehyde and conjugated dienes. During this state of GSH-depletion inducing lipid peroxidation no significant alterations in the activity of hepatic GSH-peroxidase were observed whereas the GSH-S-transferase activities towards an aryl substrate (CDNB) and an epoxide substrate (1,2-epoxy(p-nitrophenoxy)propane) significantly decreased under the treatments with VDC. The GSH-reductase activity was significantly reduced after treatment with either agent. These results clearly indicate 1st, that GSH-S-transferases are involved in the conjugation of GSH to VDC, 2nd, that GSH peroxidase remains unaffected during lipid peroxidation induced by GSH-depletion as a consequence of treatment with phorone or VDC, and 3rd, that the availability of reduced GSH may be decreased further under these conditions due to the lower content of glutathione reductase.
D000818:Animals; D004000:Dichloroethylenes / Q000494:pharmacology; D005978:Glutathione / Q000172:deficiency*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D007659:Ketones / Q000494:pharmacology; D008054:Lipid Peroxides / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D004000:Dichloroethylenes; D007659:Ketones; D008054:Lipid Peroxides; C029297:vinylidene chloride; C018637:phorone; D010544:Peroxidases; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D005978:Glutathione