Purification of folding proteins of DNA from cultured mouse mammary carcinoma cells.
We described methods of preparation of folding proteins of DNA in quantity for further characterization. In principle folding proteins were first isolated as a specific DNA-protein complex from the bulk cellular proteins by centrifugation through a sucrose density gradient, followed by dissociation or by passage through a SH-Sepharose column. The overall yield was 105 micrograms of folding proteins from 1.5 x 10(9) cells. The folding protein preparation was shown to be composed of a mixture of a few kinds of SH-containing proteins, the main components being of molecular weight of 52 K and 60 K.
D000818:Animals; D004247:DNA; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000302:isolation & purification*; D013438:Sulfhydryl Compounds / Q000032:analysis
D009363:Neoplasm Proteins; D013438:Sulfhydryl Compounds; D004247:DNA