[Isolation and study of some properties of new phosphopeptides from nerve tissue].
The phosphopeptides were extracted by organic solvents from the lipid-deleted homogenate of rat brain tissue. The latter was obtained by means of trichloroacetic acid from rats injected with 32P-NaH2PO4 into brain ventricles. Chromatography of the brain extract on DEAE-Sephadex A-25 and gel filtration through Sephadex G-25 and G-15 resulted in 3 homogenous fractions of the phosphopeptides characterized by the maximal rate of the label incorporation. These fractions appeared to be homogenous during polyacrylamide gel electrophoresis and thin-layer chromatography. The molecular weights of the fractions are equal to 1200, 1100 and 900, respectively; the isoelectric points lie at 3.2, 3.0 and 3.1, respectively. All the phosphopeptides tested contain large amounts of glycine, serine and glutamic acid and have alanine as N-terminal amino acid.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D008970:Molecular Weight; D010748:Phosphopeptides / Q000302:isolation & purification*; D051381:Rats
D000596:Amino Acids; D010748:Phosphopeptides
Vydelenie i izuchenie nekotorykh svoĭstv novykh fosfopeptidov nervnoĭ tkani.