Modification of nucleic acids with muta-carcinogenic heteroaromatic amines in vivo. Identification of modified bases in DNA extracted from rats injected with 3-amino-1-methyl-5H-pyrido[4,3-b]indole and 2-amino-6-methyldipyrido[1,2-a3:3',2'-d]imidazole.
Potent muta-carcinogens isolated from amino-acid pyrolysates, 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) and 2-amino-1-methyldipyrido[1,2-a:3',2'-d]imidazole (Glu-P-1), were injected intraperitoneally into Male Wistar rats. DNA and rRNA modified with these muta-carcinogens were then extracted from the livers of the rats, and modified nucleic-acid bases were isolated and analysed by high-performance liquid chromatography (HPLC). These modified nucleic-acid bases were identified as 3-(C8-guanyl)amino-1-methyl-5H-pyrido[4,3-b]indole (Gua-Trp-P-2) and 2-(C8-guanyl)amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole (Gua-Glu-P-1) by comparison of their retention times on HPLC and UV spectra with those of authentic compounds.
D000818:Animals; D002243:Carbolines / Q000494:pharmacology*; D002273:Carcinogens / Q000494:pharmacology*; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D007093:Imidazoles / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009153:Mutagens / Q000494:pharmacology*; D012335:RNA, Ribosomal / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D002243:Carbolines; D002273:Carcinogens; D007093:Imidazoles; D007211:Indoles; D009153:Mutagens; D012335:RNA, Ribosomal; C017821:2-amino-3-methyl-9H-pyrido(2,3-b)indole; C023785:2-amino-6-methyldipyrido(1,2-a-3',2'-d)imidazole; D004247:DNA