Growth responses of rat stomach cancer cells to gastro-entero-pancreatic hormones.
Various hormones and peptides were added to rat stomach cancer cells growing in vitro in a serum-free medium and the cell number was determined by a spectro-photometric method. Five gastro-entero-pancreatic hormones or related peptides (tetragastrin, glucagon, secretin, cholecystokinin-pancreozymin and cerulein) significantly increased the number of stomach cancer cells from 15% to 310% of the number of control cells cultivated in a serum-free, hormone-free medium. On the other hand, insulin and vasoactive intestinal peptide, and other hormones (thyroxin, epinephrine, hydrocortisone, beta-estradiol, progesterone, testosterone), peptone broth and bovine serum albumin had no significant growth effect. All the active substances belong to the two major families of gastro-entero-pancreatic polypeptide hormones, suggesting the existence of hormone receptors at the surface of stomach cancer cells.
D000818:Animals; D002452:Cell Count; D002460:Cell Line; D005768:Gastrointestinal Hormones / Q000494:pharmacology*; D009374:Neoplasms, Experimental / Q000473:pathology; D010187:Pancreatic Hormones / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013274:Stomach Neoplasms / Q000473:pathology*
D005768:Gastrointestinal Hormones; D010187:Pancreatic Hormones