Simultaneous determination of glucuronides of trimetozine in human urine by gas chromatography.
A gas chromatographic method for the simultaneous determination of four glucuronides (metabolites) of trimetozine excreted in human urine is described. The method involves pretreatment of the urine specimen [i.e. removal of interfering substances by solvent extraction, desalting on an ion-exchange (Amberlite XAD-2) column], and permethylation of glucuronides by reaction with methylsulfinyl carbanion and methyl iodide. The permethylated derivatives were submitted to gas chromatographic separation on an OV-17 column, and their structures were investigated by subsequent gas chromatographic-mass spectrometric analysis. The minimum detectable concentration of each glucuronide is 5 micrograms/ml when 1 ml of urine is used. The utility of the present method is successfully demonstrated by determining the urinary concentration of four glucuronides following oral administration of trimetozine to human subjects.
D000328:Adult; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D005965:Glucuronates / Q000652:urine*; D006801:Humans; D007475:Ion Exchange Resins; D008297:Male; D009025:Morpholines / Q000652:urine*; D011137:Polystyrenes
D005965:Glucuronates; D007475:Ion Exchange Resins; D009025:Morpholines; D011137:Polystyrenes; C005831:trimetozine; C003771:Amberlite XAD-2 resin