Identification of hepatic Z-protein in a marine elasmobranch, Platyrhinoides triseriata.
Previous studies were unable to identify Z-protein in elasmobranch liver with bromosulphophthalein as ligand. By using 8-anilinonaphthalene-1-sulphonate and Rose Bengal as ligands, however, we demonstrated in hepatic cytosol from Platyrhinoides triseriata an organic-anion-binding protein with gel-filtration characteristics identical with those of rat Z-protein. By comparison with pooled rat Z-protein, Pl. triseriata Z-protein had slightly lower affinity for 8-anilinonaphthalene-1-sulphonate and Rose Bengal, greatly decreased binding affinity for bromosulphophthalein and no binding activity for oleic acid or squalene. The Pl. triseriata Z-protein binding site was less hydrophobic than that of rat Z-protein. This observation may explain the differences in binding characteristics between the Z-proteins of these species.
D000817:Anilino Naphthalenesulfonates; D000818:Animals; D000838:Anions / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D002850:Chromatography, Gel; D003593:Cytoplasm / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins; D005399:Fishes / Q000378:metabolism*; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains
D000817:Anilino Naphthalenesulfonates; D000838:Anions; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D005456:Fluorescent Dyes; C027132:1-anilino-8-naphthalenesulfonate