A modified neutral sucrose centrifugation method for rapid detection of DNA damaged by gamma-radiation and repair in human lymphocytes.
1. The influence of 60Co gamma-radiation on the sedimentation behaviour of human lymphocyte nucleoids in neutral sucrose gradients was studied with the aid of a modified fluorescent monitor system consisting of the DNA-binding dye, Hoechst 33258 and a vertical centrifuge rotor. 2. A combined method employing the sedimentation procedure and the incorporation of tritiated nucleosides was utilized to evaluate the repair of gamma-radiated DNA.
D002499:Centrifugation, Density Gradient; D004247:DNA / Q000528:radiation effects*; D004260:DNA Repair; D005720:Gamma Rays; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects*
D004247:DNA