Maintenance of Laboratory strains of obligately anaerobic rumen bacteria.
Cultures of rumen bacteria can be stored at -20 degrees C for at least 2 years in a liquid medium containing 20% glycerol. Thawing, sampling, and refreezing do not significantly affect viability.
D000693:Anaerobiosis; D000818:Animals; D001419:Bacteria / Q000254:growth & development*; D003470:Culture Media; D005612:Freeze Drying; D007700:Kinetics; D012417:Rumen / Q000382:microbiology*
D003470:Culture Media