[Determination of total serum phospholipids by a new enzymatic colorimetric method: statistical quality control].
Enzymatic methods have progressively substituted extractive procedures in plasma lipids determination. A new enzymatic method for serum phospholipids evaluation has been recently introduced in Italy as a kit of reagents. In this method phospolipase D hydrolyzes phospholipids with free choline production which, in presence of choline-oxidase and peroxidase, is estimated by colorimetric reaction. Linearity of response and analytical variations "within-run" and "between-run" during six month observations on serum pools have been preliminarily verified.
D002794:Choline / Q000378:metabolism; D003124:Colorimetry; D006801:Humans; D010739:Phospholipase D / Q000378:metabolism; D010743:Phospholipids / Q000097:blood*; D011786:Quality Control; D011933:Reagent Kits, Diagnostic
D010743:Phospholipids; D011933:Reagent Kits, Diagnostic; D010739:Phospholipase D; D002794:Choline
Fosfolipidi totali del siero dosati con un nuovo metodo enzimatico colorimetrico: controllo statistico di qualita.