Electrical excitability of artificial enzyme membranes. III. Hysteresis and oscillations observed with immobilized acetylcholinesterase membranes.
Experimental evidence for memory and oscillations in artificial acetylcholinesterase membranes is presented. When acetylcholine is injected on one side of an artificial proteinic membrane bearing acetylcholinesterase, a potential difference is recorded as a function of time. The steady-state potential due to the enzyme activity for increasing and decreasing substrate concentrations exhibits a hysteresis loop. The non-linearity of the enzyme reaction coupled with the diffusion constraints cause also some instabilities, such as oscillations of the membrane potential.
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008564:Membrane Potentials; D008567:Membranes, Artificial; D009991:Oscillometry; D012710:Serum Albumin, Bovine
D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D012710:Serum Albumin, Bovine; D000110:Acetylcholinesterase