Binding of [14C] azaserine to DNA and protein in the rat and hamster.
The binding of [14C] azaserine or its metabolites to DNA and protein in the organs of rats and hamsters was determined at various time after treatment with [14C] azaserine. The specific activity of 14C labelling of DNA and protein was determined. Rat liver DNA and protein were most extensively labelled at 90 min post-injection, but by 24 h the specific activity decreased to the levels found in pancreas and kidney. Thymus contained negligible amounts of radioactivity at all time-points. DNA and protein from hamster pancreas contained more label than did DNA and protein from rat pancreas. The results suggest that factors other than DNA binding play a role in determining the species and organ specificity of azaserine.
D000818:Animals; D001377:Azaserine / Q000378:metabolism* / Q000494:pharmacology; D006224:Cricetinae; D004247:DNA / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D010179:Pancreas / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000378:metabolism; D013997:Time Factors
D011506:Proteins; D001377:Azaserine; D004247:DNA