Lupinosis: response of sheep to different doses of phomopsin.
Phomopsin, the mycotoxin produced by Phomopsis leptostromiformis, was found to have a very high toxicity for sheep. When administered as a single, subcutaneous injection over the dose range 1 X 25 to 98 microgram/kg body weight, all sheep given 37 X 5 microgram/kg or more died. Some, though not all, died following lower doses, the minimum lethal dose being 10 microgram/kg. The time course of hepatic response over 21 days after phomopsin administration was followed by plasma biochemical analyses including those for some enzymes (glutamate dehydrogenase, gamma-glutamyl transpeptidase, aspartate aminotransferase and alkaline phosphatase), total bilirubin and the determination of bromosulphophthalein clearance rates. Hepatobiliary impairment was apparent after all dosages of 2.5 microgram/kg and above while 1.25 microgram/kg approximated the 'no effect' level.
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001774:Blood Chemical Analysis; D004305:Dose-Response Relationship, Drug; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D009183:Mycotoxins / Q000633:toxicity*; D010939:Plant Poisoning / Q000209:etiology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000139:chemically induced*; D009842:Oligopeptides
D000469:Alkaline Phosphatase; D009183:Mycotoxins; C035753:phomopsin; D009842:Oligopeptides