New process for T-2 toxin production.
Strains of Fusarium produced high levels of T-2 toxin when cultured on certain media absorbed into vermiculite. Modified Gregory medium was nutritionally complex (2% soya meal, 0.5% corn steep liquor, 10% glucose) and, when inoculated with the appropriate fungal strain, yielded maximum T-2 toxin within 24 days of incubation at  19 degrees C. On Vogel synthetic medium N (H. J. Vogel, Microb. Genet, Bull. 13:42-43, 1956) supplemented with 5% glucose, optimal toxin levels were synthesized after incubation for 12 to 14 days at 15 degrees C. Fusarium tricinctum T-340 produced 714 and 353 mg/liter on modified Gregory medium and Vogel synthetic medium N plus 5% glucose, respectively. Improved analytical procedures were developed and involved aqueous methanol extraction, purification by liquid-liquid partitions, and gas-chromatographic quantitation.
D002849:Chromatography, Gas / Q000379:methods; D003470:Culture Media; D005670:Fusarium / Q000254:growth & development*; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D012717:Sesquiterpenes / Q000096:biosynthesis*; D013605:T-2 Toxin / Q000096:biosynthesis* / Q000302:isolation & purification; D013696:Temperature
D003470:Culture Media; D012717:Sesquiterpenes; D013605:T-2 Toxin; D005947:Glucose