Template directed reactions of 2-aminoadenylic acid derivatives.
The template-directed oligomerization of activated derivatives of 2-aminoadenylic acid (paA) on polyuridylic acid (poly(U)) in aqueous buffers was studied. The reaction differs from that of adenylic acid (pA) under identical conditions, in that only di- and tri-nucleotides are observed as substantial products rather than a longer sequence of oligomers. The reaction of paA also differs from that of pA in that it does not require Mg++, and is less susceptible to increased temperature. The relevance of these observations to the chemical evolution of polynucleotide replication is discussed. Improved syntheses of paA and its diphosphate are reported.
D000818:Animals; D008274:Magnesium; D009843:Oligoribonucleotides / Q000138:chemical synthesis; D011063:Poly A-U / Q000378:metabolism*; D011072:Poly U; D012376:Rodent Diseases / Q000235:genetics*; D013329:Structure-Activity Relationship; D013698:Templates, Genetic
D009843:Oligoribonucleotides; D011063:Poly A-U; D011072:Poly U; D008274:Magnesium