Studies on the retention of passively transferred antibodies in man. I. Quantitative measurement of the antibody activities to HBs by radioimmunoassay and its application to studies on the blood level of intravenously administered 7 S or 5 S antibody.
Measurement of diluted samples of high-titred antisera to hepatitis B surface antigen (HBs) by radioimmunoassay (RIA) proved that the RIA kit can be used quantitatively in a wider range than stated by the manufacturer. Analysis of the blood level of anti-HBs activities by RIA in a homologous system gave straight declines corresponding to the diffusion and the catabolic phases of human 7 S or 5 S antibody. The half-life of the intravenously given antibody was calculated from the catabolic decline to 17.7 days for human immunoglobulin or 2.7 days for human F(ab')2 fragment.
D006511:Hepatitis B Antigens / Q000032:analysis*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006801:Humans; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000378:metabolism*; D007275:Injections, Intravenous; D008297:Male; D011863:Radioimmunoassay
D006511:Hepatitis B Antigens; D006514:Hepatitis B Surface Antigens; D007074:Immunoglobulin G