Human allogeneic responses: lymphokine requirement for the in vitro generation of specific cytotoxic responses to a malignant melanoma cell line.
Requirements for the in vitro generation of specific cytotoxic responses by human T-lymphocytes to an allogeneic malignant melanoma cell line (MM-170) have been examined. The responder T-lymphocytes used were isolated from peripheral blood and were essentially free of non-specific cytotoxic effector cells and their precursors. MM-170 alone was found to be an inadequate stimulator of cytotoxic responses. Stimulation did occur in cultures with both MM-170 and an Interleukin-2 containing human lymphokine supernatant. The cytotoxic effector cells generated were specific for MM-170 when the MM-170 cells used for stimulation had been metabolically inactivated by glutaraldehyde treatment.
D000951:Antigens, Neoplasm / Q000276:immunology*; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D008222:Lymphokines / Q000276:immunology*; D008545:Melanoma
D000951:Antigens, Neoplasm; D008222:Lymphokines