Analysis of molecular species of phospholipids by field desorption mass spectrometry.
Various glycerophospholipids including their trimethylsilyl and t-butyldimethylsilyl derivatives and cerebroside were analysed by field desorption mass spectrometry with a silicon emitter. Choline glycerophospholipids with diacyl residues and also alk-1'-enyl and acyl residues gave the readily interpretable field desorption mass spectra in which a sodium cluster ion [M+NA]+ was a base peak. As to phosphatidylethanolamine and cerebroside, a protonated molecular ion [M+H+]+ was a base peak. The TMS and TBDMS derivatives gave a molecular ion [M]+. The field desorption analysis of natural phospholipids from myelin was carried out in comparison with the gas chromatographic mass spectrometric analysis.
D000818:Animals; D001923:Brain Chemistry; D055598:Chemical Phenomena; D002621:Chemistry; D008401:Gas Chromatography-Mass Spectrometry; D013058:Mass Spectrometry / Q000379:methods; D009186:Myelin Sheath / Q000032:analysis; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D051381:Rats
D010713:Phosphatidylcholines; D010743:Phospholipids