[Acid-stable proteinase inhibitor from the urine of pregnant women, the properties of the highly purified preparation and the isolation of a monospecific antiserum].
Two highly purified forms of acid-stable proteinase inhibitor from urine of pregnant women with nephropathies (Mr 22000 and 32000, ASI-22 and ASI-32, respectively) were obtained. The preparations were homogenous in molecular mass and polymorphous in molecular charge (pI from 3.9 to 4.2). ASI-22 an ASI-32 effectively inhibited trypsin and chymotrypsin (Ki approximately 1 x 10(-8)-1 x 10(-9) M, ka approximately 10(5)M-1 sec-1, kd approximately 3 x 10(-4) sec-1) by the permanent mechanism of action. Both forms inhibited the esterase activity of pancreatic pig elastase by the progressive mechanism of action (ki approximately 1 x 10(4)M-1 min-1 at 37 degrees). Rabbit monospecific antiserum to total ASI-22 and ASI-32 preparation was obtained 1 ml of the anti-ASI-serum contained 95 micrograms of antibodies. Total ASI preparations was immunochemically homogenous and had antigenic similarity to inter-alpha-trypsin inhibitor from human plasma.
D000818:Animals; D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D004355:Drug Stability; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification*; D007674:Kidney Diseases / Q000276:immunology / Q000652:urine; D008722:Methods; D008970:Molecular Weight; D011247:Pregnancy; D011248:Pregnancy Complications / Q000276:immunology / Q000652:urine*; D011263:Pregnancy Trimester, Third; D011480:Protease Inhibitors / Q000276:immunology / Q000302:isolation & purification / Q000652:urine*; D011817:Rabbits; D014361:Trypsin Inhibitors / Q000494:pharmacology
D007106:Immune Sera; D011480:Protease Inhibitors; D014361:Trypsin Inhibitors; D002918:Chymotrypsin
Kislotostabil'nyĭ ingibitor proteinaz iz mochi beremennykh zhenshchin; svoĭstva vysokoochishchennogo preparata; poluchenie monospetsificheskoĭ antisyvorotki.