Assay of human erythrocyte catechol-o-methyltransferase activity with naturally occurring catecholamines as substrates.
This report describes a simple and sensitive radiometric assay for catechol-o-methyltransferase activity in human erythrocytes and other tissues. Saturating concentrations of the endogenous catecholamines and S-adenosylmethionine are used under optimum assay conditions. Erythrocyte catechol-o-methyltransferase activity is also dependent on storage conditions, as activity was lost at -20 degrees C but not at -80 degrees C.
D002394:Catechol O-Methyltransferase / Q000097:blood*; D004298:Dopamine; D004837:Epinephrine; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D009638:Norepinephrine; D013379:Substrate Specificity
D002394:Catechol O-Methyltransferase; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine