[Biological properties of large-plaque clone 230 of simian adenovirus SA7(C8)].
The clone KB-230 of simian adenovirus SA7(C8) is described differing from the reference SA7(C8) strain and clones KB-2 and MB-1 by the presence of additional recognition sites when treated with different endonucleases. The KB-230 clone differs antigenically in the neutralization test from the MB-1 clone. Some biological and molecular-biological properties of the KB-230 clone were studied. All the simian cell cultures under study were highly sensitive to the cytopathic effect and replication of the KB-230 clone. The reproduction cycle of the KB-230 clone was 12 h, that of KB-2 clone 16 h. The maximum accumulation of virus in the cells was observed by 27-29 h for both clones. The KB-230 clone differed from the KB-2 clone by early development of the CPE (by 9 h of cultivation against 19 h for the latter). The oncogenic activity of the KB-230 clone was less marked than that of the MB-1 clone. The methods of heteroduplex and polypeptide analysis established the difference of the KB-230 clone from the reference SA7(C8) strain and KB-2 and MB-1 clones.
D000256:Adenoviridae / Q000276:immunology*; D000261:Adenoviruses, Simian / Q000276:immunology* / Q000472:pathogenicity; D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D003429:Cross Reactions; D003588:Cytopathogenic Effect, Viral; D006801:Humans; D009500:Neutralization Tests; D010948:Viral Plaque Assay; D014776:Virus Cultivation; D014779:Virus Replication
Nekotorye biologicheskie svoĭstva krupnobliashechnogo klona 230 adenovirusa obez'ian SA7(C8).