Effect of lymphocytes and broncho-alveolar washing cells on the replication of bovine herpesvirus type 1 in tracheal organ cultures.
The daily addition of lymphocytes collected from a calf between 7 and 11 days after experimental infection with bovine herpesvirus type 1 (BHV-1) to bovine fetal tracheal organ cultures after infection with BHV-1 did not inhibit virus replication. The daily addition of normal lymphocytes, together with a low concentration of serum antibody against BHV-1, had a slight viral inhibitory effect which was believed to be due to antibody-dependent cell-mediated cytotoxicity. The addition of broncho-alveolar washing (BAW) cells, collected before infection or 30 days after infection of a calf with BHV-1, together with lymphocyte culture supernatant, to tracheal organ cultures immediately after infection with BHV-1 produced some inhibition of virus replication. Virus replication was markedly inhibited when BAW cells collected from the calf 18 days after infection were used in a similar manner.
D000818:Animals; D001980:Bronchi / Q000502:physiology*; D002417:Cattle; D002478:Cells, Cultured; D004261:DNA Replication; D006564:Herpesviridae / Q000235:genetics*; D008214:Lymphocytes / Q000502:physiology*; D009924:Organ Culture Techniques; D011650:Pulmonary Alveoli / Q000502:physiology*; D014132:Trachea / Q000502:physiology*; D014779:Virus Replication