Formation of total, active and stable E-rosettes by human lymphocytes stimulated with con A derivatives.
Peripheral blood lymphocytes from healthy human volunteers where incubated for 48 hr with 10 micrograms/ml of native Con A or its dimeric derivatives, succinyl- and acetyl-Con A. The percentage of total-active and stable E-rosettes were determined before and after incubation. There were no differences among the three forms of the lectin. Stable E-rosettes exhibited the most dramatic effects from Con A stimulation. They increased from approximately 2% in control cultures to 20-25% in stimulated cultures. Treatment with the Con A inhibitor alpha-Methyl-D-mannoside after Con A stimulation did not affect the rosette formation. Our results suggest that redistribution of membrane receptors on stimulated lymphocytes is not responsible for increased E-rosette formation after Con A stimulation since dimeric forms of Con A are not able to induce membrane receptor redistribution.
D003208:Concanavalin A / Q000031:analogs & derivatives / Q000494:pharmacology*; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D008766:Methylmannosides / Q000494:pharmacology; D011952:Receptors, Concanavalin A / Q000187:drug effects; D012397:Rosette Formation
D008766:Methylmannosides; D011952:Receptors, Concanavalin A; D003208:Concanavalin A; C019558:succinylconcanavalin A