HPLC analyses of the aldehydic patterns produced during lipid peroxidation.
The peroxidative breakdown of membrane lipids leads to the production of several compounds with carbonyl functions. Their production has been monitored in rat liver homogenates treated with prooxidant agents namely vitamin C and ADP-Fe2+ complex. Preliminar HPLC separation of the unpolar and medium polar carbonyls produced, allowed the identification of few of them, in particular, hexanal and 4-OH-nonenal. The aim of this report is to underline the usefulness to integrate malonaldehyde evaluation with the analysis of the other carbonyls produced during lipid peroxidation.
D000244:Adenosine Diphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D008054:Lipid Peroxides / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D008054:Lipid Peroxides; C029936:adenosine diphosphate-ferric chelate; D000244:Adenosine Diphosphate; D001205:Ascorbic Acid