[Effect of artificial NaC1 and KC1 gradients on the active transport of organic acids in energy-depleted kidney proximal tubules. II. The KC1 gradient].
The stimulatory effect of KCL in bath medium on fluorescein uptake in superficial proximal tubules in the absence of Na in bath medium was observed on Na- and energy-depleted slices of the rat kidney outer cortex. In the absence of K gradient between bath medium and tubular cells, the fluorescein uptake increased with raising the intracellular K concentration. The efflux of fluorescein from the tubules was inversely proportional to K concentration in the cells. Visual control showed that at high K concentrations fluorescein was accumulated mainly in the tubular walls (i. e. in the cytoplasm of tubular cells). It is suggested that the raise of K concentration in cells results in the intracellular binding of organic acid.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism; D005452:Fluoresceins / Q000378:metabolism*; D066298:In Vitro Techniques; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D008297:Male; D011189:Potassium Chloride / Q000378:metabolism*; D051381:Rats; D012965:Sodium Chloride / Q000378:metabolism*
D005452:Fluoresceins; D012965:Sodium Chloride; D011189:Potassium Chloride
Vliianie iskusstvennykh gradientov NaC1 i KC1 na aktivnyĭ transport organicheskikh kislot v deénergizovannykh proksimal'nykh kanal'tsakh pochki. II. Gradient KC1.