The probable binding between cations and stimulatory protein kinase modulator and subsequent stimulation on cerebellar modulator-dependent protein kinases.
The formation of complexes between cations and stimulatory protein kinase modulator (PKMs) were performed by preincubation and gel filtration. The stimulatory effects of Fe3+, Fe2+, Mg2+, and Co2+ complexes on cerebellar modulator-dependent protein kinases (M-PK) were similar to those without preformation of complexes by preincubation and gel filtration. Antagonism by Ca2+ on the stimulatory effect of Mg2+ was noted in spite of the ineffectiveness of Ca2+ when present alone.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002352:Carrier Proteins / Q000378:metabolism*; D002412:Cations / Q000378:metabolism* / Q000494:pharmacology; D002531:Cerebellum / Q000201:enzymology*; D055598:Chemical Phenomena; D002621:Chemistry; D003035:Cobalt / Q000378:metabolism; D004789:Enzyme Activation; D005290:Ferric Compounds / Q000378:metabolism; D005296:Ferrous Compounds / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D008274:Magnesium / Q000378:metabolism; D008297:Male; D051379:Mice; D011494:Protein Kinases / Q000378:metabolism*
D002352:Carrier Proteins; D002412:Cations; D005290:Ferric Compounds; D005296:Ferrous Compounds; D047908:Intracellular Signaling Peptides and Proteins; C015482:protein kinase modulator; D003035:Cobalt; D011494:Protein Kinases; D008274:Magnesium; D002118:Calcium