Lipid peroxidation and haemoglobin degradation in red blood cells exposed to t-butyl hydroperoxide. Effects of the hexose monophosphate shunt as mediated by glutathione and ascorbate.
Lipid peroxidation and haemoglobin degradation were the two extremes of a spectrum of oxidative damage in red cells exposed to t-butyl hydroperoxide. The exact position in this spectrum depended on the availability of glucose and the ligand state of haemoglobin. In red cells containing oxy- or carbonmono-oxy-haemoglobin, hexose monophosphate-shunt activity was mainly responsible for metabolism of t-butyl hydroperoxide; haem groups were the main scavengers in red cells containing methaemoglobin. Glutathione, via glutathione peroxidase, accounted for nearly all of the hydroperoxide metabolizing activity of the hexose monophosphate shunt. Glucose protection against lipid peroxidation was almost entirely mediated by glutathione, whereas glucose protection of haemoglobin was only partly mediated by glutathione. Physiological concentrations of intracellular or extracellular ascorbate had no effect on consumption of t-butyl hydroperoxide or oxidation of haemoglobin. Ascorbate was mainly involved in scavenging chain-propagating species involved in lipid peroxidation. The protective effect of intracellular ascorbate against lipid peroxidation was about 100% glucose-dependent and about 50% glutathione-dependent. Extracellular ascorbate functioned largely without a requirement for glucose metabolism, although some synergistic effects between extracellular ascorbate and glutathione were observed. Lipid peroxidation was not dependent on the rate or completion of t-butyl hydroperoxide consumption but rather on the route of consumption. Lipid peroxidation appears to depend on the balance between the presence of initiators of lipid peroxidation (oxyhaemoglobin and low concentrations of methaemoglobin) and terminators of lipid peroxidation (glutathione, ascorbate, high concentrations of methaemoglobin).
D001205:Ascorbic Acid / Q000097:blood; D002263:Carboxyhemoglobin / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology; D005978:Glutathione / Q000097:blood; D006454:Hemoglobins / Q000378:metabolism*; D006600:Hexosephosphates / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008055:Lipids / Q000097:blood*; D008706:Methemoglobin / Q000378:metabolism; D010084:Oxidation-Reduction; D010108:Oxyhemoglobins / Q000378:metabolism; D010545:Peroxides; D020122:tert-Butylhydroperoxide
D006454:Hemoglobins; D006600:Hexosephosphates; D008055:Lipids; D010108:Oxyhemoglobins; D010545:Peroxides; D008706:Methemoglobin; D002263:Carboxyhemoglobin; D020122:tert-Butylhydroperoxide; D005978:Glutathione; D005033:Ethylmaleimide; D001205:Ascorbic Acid