Inhibition of histone acetylation by N-[2-(S-coenzyme A)acetyl] spermidine amide, a multisubstrate analog.
A multisubstrate analog, formed by joining coenzyme A with spermidine through an acetic acid linkage, serves as a strong inhibitor (Ki less than 10(-8) M) of the acetylation of spermidine and histones by histone acetylase purified from calf thymus. In free solutions, this analog inhibited acetylation of the various nuclear histones to a similar extent. In isolated nuclei, this analog was found to inhibit acetylation of histones H2a and H2b very much more strongly than that of histones H3 and H4.
D000107:Acetylation; D000123:Acetyltransferases / Q000037:antagonists & inhibitors; D000818:Animals; D002417:Cattle; D051548:Histone Acetyltransferases; D006657:Histones / Q000378:metabolism*; D007700:Kinetics; D029701:Saccharomyces cerevisiae Proteins; D013095:Spermidine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology
D006657:Histones; D029701:Saccharomyces cerevisiae Proteins; C035890:N-(2-(S-coenzyme A)acetyl)spermidine; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; D013095:Spermidine