Immunochemical studies of partially hydrolyzed lipopolysaccharide from Fusobacterium nucleatum ATCC 10953.
Fusobacterium nucleatum ATCC 10953 LPS was split by hydrolysis with 1 per cent acetic acid into acid-soluble polysaccharide and lipid A. Gel filtration of the acid-soluble polysaccharide on Bio-Gel P-60 gave a high-molecular-weight fraction eluted with the void volume (Vo), and a fraction eluted at 2.4 x Vo. The high-molecular-weight fraction was serologically active, and contained glucose as the only sugar. The other fraction, which was serologically inactive, contained L-glycero-D-manno-heptose, galactose, glucosamine, keto-deoxy-octonate, and phosphorus. The fraction eluted at 2.4 x Vo is thought to constitute the polysaccharide core region, whereas the high-molecular-weight fraction may represent O-antigenic side chains.
D002850:Chromatography, Gel; D005673:Fusobacterium / Q000276:immunology*; D005690:Galactose / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006385:Hemagglutination Inhibition Tests; D006539:Heptoses / Q000032:analysis; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000032:analysis* / Q000276:immunology; D008970:Molecular Weight; D013400:Sugar Acids / Q000032:analysis
D006539:Heptoses; D008070:Lipopolysaccharides; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate; D005947:Glucose; D005944:Glucosamine; D005690:Galactose