Synthesis and properties of human gamma-lipotropin.
The synthesis of human gamma-lipotropin by the solid-phase method is described. The synthetic product was characterized by Rf in partition chromatography on Sephadex G-50, paper electrophoresis, polyacrylamide gel electrophoresis, thin-layer chromatography, HPLC, end group determination, peptide mapping of a tryptic digest, and amino acid analyses of acid and enzymic digests. The synthetic material is identical to natural human gamma-lipotropin when assayed against natural human beta-lipotropin for lipolytic activity.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001681:Biological Assay; D006801:Humans; D007202:Indicators and Reagents; D008066:Lipolysis / Q000187:drug effects; D013329:Structure-Activity Relationship; D008083:beta-Lipotropin / Q000138:chemical synthesis* / Q000494:pharmacology
D000596:Amino Acids; D007202:Indicators and Reagents; D008083:beta-Lipotropin