Chemical fingerprinting of non-acid glycosphingolipids in meconium of a human individual of blood group B Le(a-b+) and secretor.
In an attempt to elucidate blood group polymorphism systematically in human intestinal tissue, glycosphingolipids of meconia of single individuals have been studied. In this report we describe the major glycosphingolipids in meconium of a newborn typed as a B Le(a-b+) secretor. The glycolipids were analysed as four subfractions obtained after deacetylation of fractions from silicic acid column chromatography of the peracetylated total non-acid glycolipid extract. Chemical characterization (mass spectrometry and 1H-NMR spectroscopy of permethylated and LiA1H4-reduced permethylated derivatives) and immunological characterization (haemagglutination-inhibition of native glycolipids in the liposome form) revealed a very complex pattern of fucosyl-containing glycolipids, probably the result of several blood group glycosyltransferase activities. The major fucolipids, all based on lactotetraosylceramide, had a characteristic ceramide of mainly phytosphingosine and 2-hydroxy fatty acids with 16 and 20-24 carbon atoms.
D000017:ABO Blood-Group System; D002855:Chromatography, Thin Layer; D006028:Glycosphingolipids / Q000032:analysis*; D006801:Humans; D007231:Infant, Newborn; D007983:Lewis Blood Group Antigens; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008470:Meconium / Q000032:analysis*; D011110:Polymorphism, Genetic
D000017:ABO Blood-Group System; D006028:Glycosphingolipids; D007983:Lewis Blood Group Antigens