Adaptative features of ectothermic enzymes. III--Studies on phosphoglucose isomerase (PGI) from five species of tropical fishes of the superorder Ostariophysi.
1. The electrokinetic and thermostability properties of phosphoglucose isomerase (PGI) in five species of tropical fish (Ostariophysi)-Leporinus friderici, L. silverstrii, Schizodon nasuttus, Hypostomus sp and Pimelodus maculatus--have been studied in order to study the adaptative nature of protein heterogeneity found in ectotherms. 2. Unlike most diploid fishes, the PGI of these species seemed to be encoded by three, four or five loci. 3. The subunits encoded by these loci occurred at different levels in the different tissues and organs analyzed. 4. Genetic variants at the three PGI loci were detected in two species of the family Anostomidae (L. friderici and S. nasuttus). In the family Pimelodidae, P. Maculatus showed a genetic variant as the Pgi-1 locus. 5. The product of these loci could be separated in three PGI regions based on their electrophoretic mobility, tissue distribution and thermostability properties. 6. The more anodal region (including isozymes and allozymes) was more thermolabile then the less anodal one, which is predominant in a single tissue-skeletal muscle.
D000818:Animals; D001921:Brain / Q000201:enzymology; D004355:Drug Stability; D005260:Female; D005399:Fishes / Q000378:metabolism*; D005956:Glucose-6-Phosphate Isomerase / Q000302:isolation & purification*; D006066:Gonads / Q000201:enzymology; D007527:Isoenzymes / Q000302:isolation & purification; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D010641:Phenotype; D013045:Species Specificity; D014329:Tropical Climate
D007527:Isoenzymes; D005956:Glucose-6-Phosphate Isomerase