[Sulfhydryl groups of alpha-actinin].
It has been established that the molecule of alpha-actinin (m. w. 200 000) contains 16 SH-groups, which are distinguished by their activity. Six SH-groups may be comparatively easily discovered in the native protein. Four partly "masked" SH-groups are titrated in the low acidic media. Five-six SH-groups are completely masked and are revealed only after denaturation of protein. Blocking of SH-groups of alpha-actinin by the parachloromercuribenzoate decreases the biological activity of alpha-actinin measured by the formation of the alpha-actinin--actin complex. It has been shown that partly "masked" SH-groups revealed with the temperature increase are not incorporated into the active centre and do not take part in protein functioning.
D000185:Actinin / Q000378:metabolism*; D000199:Actins / Q000378:metabolism; D000818:Animals; D002729:Chloromercuribenzoates / Q000494:pharmacology; D007700:Kinetics; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism; D011817:Rabbits; D013438:Sulfhydryl Compounds / Q000032:analysis; D020245:p-Chloromercuribenzoic Acid
D000199:Actins; D002729:Chloromercuribenzoates; D009124:Muscle Proteins; D013438:Sulfhydryl Compounds; D000185:Actinin; D020245:p-Chloromercuribenzoic Acid
Sul'fgidril'nye gruppy al'fa-aktinina.