Isocratic high-performance liquid chromatographic method for the determination of tricyclic antidepressants and metabolites in plasma.
An isocratic high-performance liquid chromatographic method for the determination of six tricyclic antidepressants and their major metabolites is presented. Hexane containing 0.5% diethylamine was used as an extraction solvent to minimize adsorption onto glass. A reversed-phase cyanopropylsilane column was used with a mobile phase consisting of 70% acetonitrile and 30% 0.03 M acetate buffer, pH 7.0. Good identification and quantitation were obtained by the use of both UV detection at 245 nm and spectrofluorometric detection with an excitation wavelength of 276 nm and an emission filter with a 370-nm cutoff. A minimum detectable limit of less than 5 ng/ml of plasma is possible with this system. The reproducibility and precision of the method are shown from the analysis of samples containing 20-400 ng/ml in plasma.
D000327:Adsorption; D000929:Antidepressive Agents, Tricyclic / Q000097:blood*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005898:Glass; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000929:Antidepressive Agents, Tricyclic