Blotting of RNA onto ion exchange paper allowing subsequent characterization by in situ translation in addition to blot hybridization.
We present the preparation of an ion exchange paper which can be used as a solid carrier in the transfer of RNA from gels. In addition to detection by blot hybridization to specific probes, transferred RNA can be characterized by cell-free translation in situ. Preparation of the paper is simple, inexpensive and reproducible. Examples of applications are shown and possible other applications are discussed.
D000818:Animals; D002474:Cell-Free System; D002852:Chromatography, Ion Exchange / Q000379:methods; D008979:Moloney murine leukemia virus / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012156:Reticulocytes / Q000378:metabolism
D012333:RNA, Messenger; D012367:RNA, Viral