Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate.
n-Prop]yl gallate (0.1 to 0.25 molar, in glycerol) reduces by a factor of 10 the rate of fading of fluorescence of cell structures labeled with tetramethylrhodamine or fluorescein-conjugated antibodies. Hence, prolonged photographic exposure of immunofluorescently labeled cells in the fluorescence microscope yields images with increased sensitivity, making feasible multiple data collection, as with serial optical sectioning.
D019793:Fluorescein; D005452:Fluoresceins; D005609:Free Radicals; D005707:Gallic Acid / Q000031:analogs & derivatives*; D008856:Microscopy, Fluorescence / Q000379:methods*; D010777:Photochemistry; D011435:Propyl Gallate; D012235:Rhodamines; D014966:Xanthenes
D005452:Fluoresceins; D005609:Free Radicals; D012235:Rhodamines; D014966:Xanthenes; D005707:Gallic Acid; D011435:Propyl Gallate; D019793:Fluorescein