The induction of Ouar-mutations in nontransformable CVP3SC6 mouse fibroblasts.
A line of C3H mouse fibroblasts designated CVP3SC6 (CVP) was found to be nonresponsive to the induction of morphological transformation by the polycyclic aromatic hydrocarbon benzo[a]pyrene (BaP) and its BaP-7,8-diol and BaP-diol-epoxide derivates. This cell line was, however, susceptible to BaP-induced mutation to ouabain resistance (Ouar). Concentrations of BaP between 0.63 and 5 microM caused an 11- to 370-fold increase in the frequency of Ouar cells relative to that found in untreated cultures. Isolates of Ouar cells were at least 10,000-fold more resistant to the cytotoxic effects of Oua and exhibited a stable resistant phenotype after 120 days of growth in the absence of the selective agent.
D000818:Animals; D001580:Benzopyrenes / Q000494:pharmacology* / Q000633:toxicity; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D008297:Male; D051379:Mice; D009154:Mutation; D010042:Ouabain / Q000494:pharmacology
D001580:Benzopyrenes; D010042:Ouabain