The preparation of orosomucoid from nephrotic urine.
A gel chromatographic and ion exchange procedure is described for the preparation of orosomucoid from nephrotic urine. The removal of endogenous lipid and glycosaminoglycan than yields a highly homogeneous preparation. Aggregates are formed at ambient temperatures and as a result of lyophilisation of ultrafiltration.
D005612:Freeze Drying; D006025:Glycosaminoglycans / Q000652:urine; D006801:Humans; D008055:Lipids / Q000652:urine; D009401:Nephrosis / Q000652:urine*; D009961:Orosomucoid / Q000652:urine*; D011485:Protein Binding
D006025:Glycosaminoglycans; D008055:Lipids; D009961:Orosomucoid