Assessment of a radioisotopic assay for vitamin B12 using an intrinsic factor preparation with R proteins blocked by vitamin B12 analogues.
A competitive protein binding radioassay kit for serum vitamin B(12) has been assessed. Precision, linearity, sensitivity, and specificity have been found to be satisfactory. Falsely-normal assay results in patients with vitamin B(12) deficiency have not been observed.
D000293:Adolescent; D001681:Biological Assay; D005260:Female; D006801:Humans; D007437:Intrinsic Factor; D008297:Male; D008875:Middle Aged; D011869:Radioligand Assay; D011933:Reagent Kits, Diagnostic; D014155:Transcobalamins / Q000037:antagonists & inhibitors; D014805:Vitamin B 12 / Q000031:analogs & derivatives / Q000097:blood*; D014806:Vitamin B 12 Deficiency / Q000097:blood
D011933:Reagent Kits, Diagnostic; D014155:Transcobalamins; D007437:Intrinsic Factor; D014805:Vitamin B 12