Factors regulating the steroid 11-hydroxylation by non-germinating spores of Cunninghamella elegans (Lendner).
In the presence of malate or citrate sporangiospores of C. elegans were able to hydroxylate cortexolone with a rate twofold exceeding that of the control, water suspended spores. Analysis of the intracellular nicotinamide coenzyme pools revealed an increased NADPH:(NADP+ + NADPH) ratio, indicating more effective NADPH-generating systems in malate- or citrate-stimulating spores. Swollen spores remaining in the pregermination state, retained higher cortexolone-hydroxylating activity in the absence of malate and citrate. In these spores degradation of endogenous alanine and glutamic acid was observed. Possible NADPH-generating systems in C. elegans sporangiospores were discussed.
D015065:17-Hydroxycorticosteroids / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D002951:Citrates / Q000494:pharmacology*; D003350:Cortodoxone / Q000378:metabolism*; D006854:Hydrocortisone / Q000096:biosynthesis; D006900:Hydroxylation; D008293:Malates / Q000494:pharmacology*; D009090:Mucorales / Q000187:drug effects* / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D013172:Spores, Fungal / Q000187:drug effects / Q000378:metabolism; D013237:Stereoisomerism
D015065:17-Hydroxycorticosteroids; D000596:Amino Acids; D002951:Citrates; D008293:Malates; D009243:NAD; D009249:NADP; D003350:Cortodoxone; D006854:Hydrocortisone