Gelatin-reactive protein in human serum--bioassay, characterization and clinical significance.
We investigated the adherence of gelatinized lipid emulsion (GLE) to tissues and cells, the reaction system of this phenomenon and the clinical significance of the adherence-promoting factor (APF) in human serum. In the presence of fresh human serum, GLE adhered to all tested tissues and blood cells. APF was shown to be macromolecular alpha 2-globulin; it formed flocculent precipitate with gelatin, and adsorbed to gelatin-Sepharose. Therefore, we termed APF gelatin-reactive protein (GRP) although it may be identical or closely related to plasma fibronectin. We also noted a significant decrease in GRP activity in patients with systemic disorders including cancer, liver cirrhosis, and systemic lupus erythematosus when their sera were assayed by the GLE-rat liver system.
D000818:Animals; D001681:Biological Assay; D001798:Blood Proteins / Q000302:isolation & purification*; D002448:Cell Adhesion / Q000187:drug effects; D004655:Emulsions; D005780:Gelatin; D006801:Humans; D007122:Immunoelectrophoresis; D008055:Lipids; D008099:Liver / Q000187:drug effects; D008103:Liver Cirrhosis / Q000097:blood; D008180:Lupus Erythematosus, Systemic / Q000097:blood; D009369:Neoplasms / Q000097:blood; D051381:Rats; D013045:Species Specificity
D001798:Blood Proteins; D004655:Emulsions; D008055:Lipids; D005780:Gelatin