[Detection of alpha-naphthyl acetate esterase in Francisella tularensis].
The activity of alpha-naphthyl-acetate esterase (alpha-NAE) has been studied in Francisella tularensis strains belonging to 3 subspecies. Alpha-NAE has been detected spectrophotometrically in native cells and cell extracts of the virulent nonarctic strains Schu and Cole, holarctic virulent strain No. 503/841 and holarctic vaccine strain No. 15/10, Central Asian virulent strain No. 543, as well as their attenuated variants. In all the strains studied the presence of 7 alpha-NAE isoenzymes has been established by means of electrophoresis in acrylamide gel, and the relative electrophoretic activity and the enzymatic activity of these isoenzymes have been determined. The nonarctic and holarctic strains have been found to differ from the Central Asian strain in the activity of 2 alpha-NAE isoenzymes, named characteristic isoenzymes. After the attenuation of the strains belonging to all subspecies the enzymatic composition of these strains remained unchanged, but at the same time the total activity of the holarctic and Central Asian strains increased, while that of the nonarctic strains decreased. These differences in the activity of alpha-NAE isoenzymes, if confirmed in further studies on a greater number of strains belonging to 3 Francisella tularensis subspecies, can be used as an additional sign for differentiation.
D002265:Carboxylic Ester Hydrolases / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005604:Francisella tularensis / Q000201:enzymology* / Q000472:pathogenicity; D007527:Isoenzymes / Q000032:analysis / Q000302:isolation & purification; D009283:Naphthol AS D Esterase / Q000032:analysis / Q000302:isolation & purification*; D013045:Species Specificity; D013053:Spectrophotometry; D014774:Virulence
D007527:Isoenzymes; D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases
Obnaruzhenie al'fa-naftilatsetatésterazy u Francisella tularensis.