Subcellular distribution, and physical and immunological properties of hepatic alanine: glyoxylate aminotransferase isoenzymes in different mammalian species.
1. Subcellular distribution, and physical and immunological properties of hepatic alanine: glyoxylate aminotransferase isoenzymes 1 and 2 were examined with ten different mammalian species. 2. Intracellular organelles containing isoenzyme 1 varied from species to species; isoenzyme 1 was located in the peroxisomes, mitochondria or both organelles. In contrast, isoenzyme 2 was found only in the mitochondria but not in the peroxisomes. 3. In any species, isoenzyme 1 had molecular weight of approx. 80,000 with two identical subunits, and isoenzyme 2 approx. 200,000 with four identical subunits. 4. In any species, an immunological cross-reactivity was observed among isoenzymes 1 and among isoenzymes 2 but did not between isoenzymes 1 and isoenzymes 2.
D000410:Alanine Transaminase / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000918:Antibody Specificity; D002415:Cats; D002417:Cattle; D004285:Dogs; D006168:Guinea Pigs; D007527:Isoenzymes / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008251:Macaca; D008297:Male; D051379:Mice; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013347:Subcellular Fractions / Q000378:metabolism; D013552:Swine; D000637:Transaminases
D007527:Isoenzymes; D000637:Transaminases; D000410:Alanine Transaminase; C019635:Alanine-glyoxylate transaminase