In vitro O-methylation of 4-hydroxyestrone monosulfates.
In vitro O-methylation of 4-hydroxyestrone monosulfates has been examined by means of high-performance liquid chromatography with electrochemical detection. When 4-hydroxyestrone or its 3-sulfate was incubated with rat liver homogenate in the presence of S-adenosyl-L-methionine, the 4-methyl ether was formed in twelve times larger amount than the 3-methyl ether. 4-Hydroxyestrone 4-sulfate served for O-methylation to much less extent as a substrate namely, only a small amount of the 4-methyl ether was formed. Enzymic sulfation of 4-hydroxyestrone with rat liver 105000 g supernatant fortified with adenosine 3'-phosphate 5'-phosphosulfate provided solely catechol estrogen 4-sulfates. The participation of catechol O-methyltransferase and aryl sulfatase in the formation of guaiacol estrogens has been discussed.
D000818:Animals; D002394:Catechol O-Methyltransferase / Q000378:metabolism; D004970:Estrone / Q000031:analogs & derivatives*; D006139:Guaiacol / Q000378:metabolism; D006894:Hydroxyestrones / Q000378:metabolism*; D008297:Male; D008745:Methylation; D051381:Rats; D011919:Rats, Inbred Strains; D013431:Sulfates / Q000378:metabolism
D006894:Hydroxyestrones; D013431:Sulfates; D004970:Estrone; C026418:4-hydroxyestrone; D006139:Guaiacol; D002394:Catechol O-Methyltransferase