Simultaneous determination of dantrolene and its metabolites, 5-hydroxydantrolene and nitro-reduced acetylated dantrolene (F 490), in plasma and urine of man and dog by high-performance liquid chromatography.
A reversed-phase high-performance liquid chromatographic method is described for the simultaneous determination of the skeletal muscle relaxant dantrolene and its metabolites, 5-hydroxydantrolene and nitro-reduced acetylated dantrolene (F-490), in plasma and urine of man and dog. The substances are detected spectrophotometrically at 375 nm. The detection limits are 0.02 mg/l. A preliminary extraction step into a choloroform-butanol mixture is required for the plasma samples. The method is suitable for pharmacokinetic studies of dantrolene.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003620:Dantrolene / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000652:urine; D004285:Dogs; D006801:Humans; D008297:Male
C034271:5-hydroxydantrolene; C036127:F 490; D003620:Dantrolene