Effect of phospholipase A2 on calcium transport in brain synaptosomes.
Pretreatment of isolated brain synaptic endings with commercial phospholipase A2 isolated from venom of Apis mellifera, followed by a BSA washing, selectively inhibited the depolarization-dependent portion of 45Ca uptake. Phospholipase A2 initially caused an increase of synaptosome respiration and subsequently inhibited their oxygen uptake, but this effect was completely abolished in BSA-containing media. The classical uncoupler of oxidative phosphorylation, DNP, produced release of 45Ca or [3H]GABA from superfused synaptosomes previously loaded with radioactive calcium or GABA. The treatment of synaptosomes with phospholipase A2 had no effect on the spontaneous efflux of 45Ca or [3H]GABA. However, depolarization-dependent release of [3H]GABA from synaptosomes treated with phospholipase A2 was significantly inhibited. We suggest that the inhibition of depolarization-dependent influx of 45Ca into synaptosomes treated with phospholipase A2 may be attributed to the lesion of the specific function of plasma membrane rather than to the impairment of the calcium-sequestrating function of intraterminal structures.
D019297:2,4-Dinitrophenol; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects; D004140:Dinitrophenols / Q000494:pharmacology; D005227:Fatty Acids / Q000032:analysis; D066298:In Vitro Techniques; D010101:Oxygen Consumption / Q000187:drug effects; D010740:Phospholipases / Q000494:pharmacology*; D010741:Phospholipases A / Q000494:pharmacology*; D054467:Phospholipases A2; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism*; D014475:Uncoupling Agents / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D004140:Dinitrophenols; D005227:Fatty Acids; D014475:Uncoupling Agents; D005680:gamma-Aminobutyric Acid; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D019297:2,4-Dinitrophenol; D002118:Calcium