Expression of a chronic granulomatous disease-like defect by fluoride-exhausted neutrophils.
Neutrophils incubated with 20 mM F- express a respiratory burst without degranulating or performing phagocytosis. After 60 min of F- treatment, the burst is exhausted and cannot be restarted. Neutrophils so treated have a microbicidal defect similar to that of chronic granulomatous disease (CGD): they kill Str. mitis at a nearly normal rate, but show a marked impairment in the destruction of S. aureus. They differ from CGD neutrophils in that they also display a defect in motility. This defect, however, is not so severe as to seriously impair their ability to kill bacteria by mechanisms that are independent of endogenously generated microbicidal oxidants.
D001770:Blood Bactericidal Activity / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects; D003594:Cytoplasmic Granules; D005459:Fluorides / Q000494:pharmacology*; D006105:Granulomatous Disease, Chronic / Q000097:blood*; D006801:Humans; D007455:Iodine / Q000378:metabolism; D007773:Lactates / Q000096:biosynthesis; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects
D007773:Lactates; D007455:Iodine; D005459:Fluorides; D010100:Oxygen