Serological studies in patients on platelet- and granulocyte-substitution therapy.
A serological follow-up study was undertaken in 47 patients with bone-marrow failure, who were repeatedly transfused with random donor granulocytes and/or platelets. Sera, obtained at regular intervals, were investigated in the leucoagglutination test, the lymphocytotoxic test and the immunofluorescence test on paraformaldehyde-fixed platelets, granulocytes and lymphocytes. The frequency of alloimmunization was high (73%). Not only HLA antibodies, but also blood-cell-specific alloantibodies were detected in the sera of the alloimmunized patients, e.g. lymphocyte-specific, platelet-specific and granulocyte-specific antibodies. The immunofluorescence test on platelets was also used as a crossmatch, and when this test was positive it was always found that after platelet transfusion the increment value was nil.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001788:Blood Grouping and Crossmatching; D001792:Blood Platelets / Q000276:immunology; D001803:Blood Transfusion; D001855:Bone Marrow Diseases / Q000628:therapy*; D002648:Child; D006098:Granulocytes / Q000276:immunology / Q000637:transplantation*; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007518:Isoantibodies / Q000096:biosynthesis*; D008214:Lymphocytes / Q000276:immunology; D008875:Middle Aged; D017713:Platelet Transfusion; D011446:Prospective Studies
D006680:HLA Antigens; D007518:Isoantibodies