The reconstitution of histone H3-H4 tetramer from acid extracted histones in the absence of urea.
Simple mixing of acid purified histones H3 and H4 in equimolar quantities at low ionic strength near pH 7 does not yield the tetramer but rather a high Mr aggregate. Dialysis of acid extracted total or core histones into 2 M NaCl 150 mM phosphate (pH 7.4) followed by fractionation of the histone complexes at lower ionic strength (150 mM NaCl) results in an H3-H4 tetramer of a structure identical to that derived from salt-extracted histones. Dialysis of acid extracted total or core histones directly into the lower ionic strength buffer with subsequent fractionation, results in H3-H4 tetramer of closely similar structure.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D006657:Histones / Q000302:isolation & purification; D046911:Macromolecular Substances; D008970:Molecular Weight; D014508:Urea
D006657:Histones; D046911:Macromolecular Substances; D014508:Urea