Neuronal mapping: a photooxidation reaction makes Lucifer yellow useful for electron microscopy.
Irradiation Lucifer yellow-filled neurons with intense blue light in the presence of 3,3'-diaminobenzidine produces an electron-opaque osmiophilic polymer within the injected cells. This technique is valuable when cobalt or horseradish peroxidase injections are difficult or when a second intracellular marker is needed to demonstrate neuronal contacts.
D015100:3,3'-Diaminobenzidine; D000818:Animals; D003400:Astacoidea; D005724:Ganglia / Q000502:physiology / Q000648:ultrastructure; D066298:In Vitro Techniques; D007546:Isoquinolines; D007865:Leeches; D008027:Light; D008854:Microscopy, Electron / Q000379:methods; D009474:Neurons / Q000502:physiology / Q000648:ultrastructure*; D013569:Synapses / Q000648:ultrastructure
D007546:Isoquinolines; D015100:3,3'-Diaminobenzidine; C017475:lucifer yellow