Glial fibrillary acidic protein synthesized in vitro using messenger RNA from jimpy mouse spinal cord.
Glial fibrillary acidic (GFA) protein was synthesized in vitro in a rabbit reticulocyte lysate system programmed with messenger RNA (mRNA) extracted from Jimpy mouse spinal cord. It was identical in molecular weight and charge to that synthesized from normal mouse mRNA and GFA protein extracted from normal mouse cord. These data suggest that the Jimpy mutation does not affect the primary phenotypic expression of GFA protein.
D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D051379:Mice; D008816:Mice, Jimpy; D009186:Myelin Sheath / Q000378:metabolism*; D009900:Optic Nerve / Q000378:metabolism; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism*; D012156:Reticulocytes / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism*
D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D012333:RNA, Messenger