Rosette inhibition test: a multicentre investigation of early pregnancy factor in humans.
The rosette inhibition test for the detection of early pregnancy factor is described in detail. The extended methodology presented here represents the cumulative experience of three independent laboratories. Special reference is made to the effect on the assay of varying the conditions of rosette formation between lymphocytes and sheep red blood cells. Antilymphocyte sera prepared for use in the rosette inhibition test fell into three categories: (i) with no rosette inhibiting activity, (ii) with rosette inhibiting activity which is not affected by the presence of EPF, and (iii) rosette inhibiting activity which is significantly increased in the presence of EPF. To date, this third reaction has been found to be a specific indication of the presence in serum of early pregnancy factor.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D018835:Chaperonin 10; D005260:Female; D006041:Goats; D006168:Guinea Pigs; D006736:Horses; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D010455:Peptides; D011247:Pregnancy; D011257:Pregnancy Proteins; D011817:Rabbits; D012397:Rosette Formation / Q000379:methods*; D013491:Suppressor Factors, Immunologic
D000961:Antilymphocyte Serum; D018835:Chaperonin 10; D007166:Immunosuppressive Agents; D010455:Peptides; D011257:Pregnancy Proteins; D013491:Suppressor Factors, Immunologic; C025124:early pregnancy factor