Survival and growth of Campylobacter fetus subsp. jejuni on meat and in cooked foods.
Twelve strains of Campylobacter fetus subsp. jejuni isolated from humans and animals grew at temperatures ranging from 34 to 45 degrees C and pH minima between 5.7 and 5.9. Only one strain grew at pH 5.8 with lactic acid present at a concentration similar to that in meat. All strains had decimal reduction times of less than 1 min at 60 degrees C. Further examination of a typical strain showed that it grew at 37 degrees C on high-pH meat but not at 37 degrees C on normal-pH meat. Bacterial numbers on both high (6.4)-pH and normal (5.8)-pH inoculated meat declined at a similar rate when the meat was stored at 25 degrees C. At -1 degree C, the rate of die-off was somewhat slower on normal-pH meat but was very much slower on high-pH meat. The initial fall in bacterial numbers that occurred when meat was frozen was also greater for normal-pH meat than for high-pH meat. The organism exhibited a long lag phase (1 to 2 days) when grown in cooked-meat medium at 37 degrees C and died in meat pies stored at 37 or 43 degrees C. Evaluation of the risk of Campylobacter contamination of red-meat carcasses to human health must take into account the limited potential of the organism to grow or even survive on fresh meats and in warm prepared foods.
D000818:Animals; D002167:Campylobacter / Q000254:growth & development*; D002168:Campylobacter fetus / Q000254:growth & development* / Q000302:isolation & purification; D002455:Cell Division; D002470:Cell Survival; D003296:Cooking; D005516:Food Microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008460:Meat; D013045:Species Specificity; D013696:Temperature