[Study of the thiamine supply of healthy school-age children and adolescents by examining transketolase activity and the TDP effect in the blood].
A modified method is described for determination of transketolase (TK) activity in the blood and the degree of its stimulation under the effect of exogenous thiamine diphosphate (TDP-effect). The method allows for an appreciable reduction of the time required for an analysis and to decrease 2-fold the blood volume necessary for the determination. With the use of the modification described 70 schoolchildren of three age groups (7-10, 11-13 and 14-15 years old) attending one of the Moscow non-specialized prolonged-day schools were examined. It was found that the magnitude of TDP-effect in the schoolchildren of all three groups was fairly similar (9.4, 7.7 and 6.9%, respectively) and was within normal (15%). Despite adequate thiamine status of all the group on the whole, in part of the schoolchildren (in 20% on the average) of each age group the magnitude of TDP-effect exceeded 15%. This pointed to inadequate supply with thiamine of these schoolchildren and to the necessity of taking appropriate measures aimed at preventing the clinically marked thiamine deficiency. The modified method suggested may be used during studies on thiamine supply of different population groups, particularly of the schoolchildren attending non-specialized schools.
D000293:Adolescent; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D008403:Mass Screening; D013832:Thiamine Deficiency / Q000097:blood*; D013835:Thiamine Pyrophosphate; D014174:Transketolase / Q000097:blood*
D014174:Transketolase; D013835:Thiamine Pyrophosphate
Izuchenie obespechennosti zdorovykh deteĭ podrostkov shkol'nogo vozrasta tiaminom putem opredeleniia aktivnosti transketolazy i TDF-éffekta v krovi.