Electron microscopic investigations on the upper respiratory tracts of infants and children for detecting viral infections.
The upper respiratory tract of 20 infants and children, suffering from viral diseases, have been investigated cytochemically and electron microscopically. We employed the electron microscope negative staining and the concanavalin-A-peroxidase methods in our investigations, besides the usual serological and immunological ones. Concanavalin-A-peroxidase reaction showed a characteristic damaging of the cell-surface which may play an important role in viral infection. The electron microscope negative staining investigations showed adeno-, herpes- and influenza-virions which are referring to viruses. These morphological methods are useful and they increase the efficiency of diagnosing viral infections.
D002462:Cell Membrane / Q000648:ultrastructure; D002648:Child; D002675:Child, Preschool; D003208:Concanavalin A / Q000494:pharmacology; D006651:Histocytochemistry; D006801:Humans; D007223:Infant; D008853:Microscopy; D010544:Peroxidases / Q000494:pharmacology; D012137:Respiratory System / Q000648:ultrastructure*; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000473:pathology*; D014777:Virus Diseases / Q000175:diagnosis / Q000473:pathology*
D003208:Concanavalin A; D010544:Peroxidases