Monoclonal antibodies to the glucocorticoid receptor.
One thousand-fold purified unactivated rat liver glucocorticoid receptor was obtained by affinity chromatography followed by gel filtration. Splenic lymphocytes of immunized mice were fused with a nonproducer SP2 myeloma cell line to obtain hybridomas secreting antibodies against receptor. Hybridoma lines, injected into mice, developed ascites tumors which provide substantial amounts of monoclonal antibodies. A monoclonal antibody was purified from ascites fluids by chromatography on an anti-mouse IgG column. The antibody was shown to react physically with the glucocorticoid receptor.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000276:immunology* / Q000302:isolation & purification; D011987:Receptors, Steroid / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid