In vitro selection of tumor cells obtained from patients with ovarian carcinoma. A cytogenetic study.
Five cases of ovarian carcinoma were studied cytogenetically. Using cell cultures derived from ascitic effusions, two cytogenetically distinguishable cell populations were found in each case: one in the diploid range, and one in the heteroploid range. Selection in vitro occurred against the heteroploid cells. As a result of these findings, it is suggested that chromosome analyses can diminish the possibility of overlooking cellular changes leading to cell populations not representative of the carcinoma in vivo.
D000230:Adenocarcinoma / Q000235:genetics; D000231:Adenocarcinoma, Papillary / Q000235:genetics; D000368:Aged; D001202:Ascitic Fluid / Q000166:cytology; D002478:Cells, Cultured; D002871:Chromosome Banding; D003536:Cystadenocarcinoma / Q000235:genetics; D004171:Diploidy; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007621:Karyotyping; D008677:Metaphase; D008875:Middle Aged; D008938:Mitosis; D010051:Ovarian Neoplasms / Q000235:genetics*