The effect of weak bases on lysosomal enzyme secretion by mononuclear phagocytes.
NH4Cl induces a dose-dependent secretion of lysosomal enzymes by mouse peritoneal macrophages and human peripheral blood monocytes. The mechanism of NH4Cl-stimulated hexosaminidase release is distinct from that initiated by the inflammatory stimulus, zymosan. The spontaneous lysosomal secretion of the continuous murine macrophage-like cell line, P388D1, is inhibited by up to 50% in the presence of NH4Cl and other weak bases.
D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D046508:Culture Techniques; D006596:Hexosaminidases / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology*; D008264:Macrophages / Q000201:enzymology*; D051379:Mice; D010586:Phagocytes / Q000201:enzymology*; D015054:Zymosan / Q000494:pharmacology
D000643:Ammonium Chloride; D015054:Zymosan; D007770:L-Lactate Dehydrogenase; D006596:Hexosaminidases