Colchicine resistance in human cell lines. Pleiotropic phenotype and decreased membrane permeability.
Colchicine-resistant human cells were initially observed in patients exhibiting C-anaphases or tetraploidy in lymphocyte cultures. Cell lines established from these patients displayed cross-resistances to daunomycin, emetine, vinblastine, and vincristine and collateral sensitivity to Xylocaine, showing a pleiotropic phenotype similar to that described in permeability mutants in CHO cells. 3H colchicine uptake and binding assays confirmed a decreased permeability to the drug.
D002460:Cell Line; D003078:Colchicine / Q000494:pharmacology*; D003429:Cross Reactions; D003513:Cycloheximide / Q000494:pharmacology; D003630:Daunorubicin / Q000494:pharmacology; D004351:Drug Resistance; D004640:Emetine / Q000494:pharmacology; D006801:Humans; D008012:Lidocaine / Q000494:pharmacology; D010641:Phenotype; D012867:Skin; D014747:Vinblastine / Q000494:pharmacology; D014750:Vincristine / Q000494:pharmacology
D014750:Vincristine; D014747:Vinblastine; D003513:Cycloheximide; D008012:Lidocaine; D003078:Colchicine; D004640:Emetine; D003630:Daunorubicin