[Uracil-DNA-glycosylase in the rat: correlation between the enzyme activity and rate of DNA synthesis in different tissues].
Uracil-DNA-glycosylase which releases uracil residues from DNA has been purified from rat liver more than 300-fold. The enzyme has a molecular weight of about 28,0000, Km = 1.7 . 10(-9) M. The content of uracil-DNA-glycosylase in five different tissues of the rat is correlated with the rate of DNA synthesis in the tissues. The enzyme activity in rat liver increases more than 3 times after partial hepatectomy, showing a peak 28-32 hrs following the surgery. The results obtained suggest that uracil-DNA-glycosylase from mammalian cells releases uracil residues from DNA at time intervals close to those of replication.
D000818:Animals; D045647:DNA Glycosylases; D004261:DNA Replication; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008115:Liver Regeneration; D008297:Male; D008970:Molecular Weight; D009699:N-Glycosyl Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D009928:Organ Specificity; D051381:Rats; D051981:Uracil-DNA Glycosidase
D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase
Uratsil-DNK-glikozilaza krysy: korreliatsiia aktivnosti fermenta i sinteza DNK v razlichnykh tkaniakh.