Chromatographic assay of neomycin B and C in neomycin sulfate powders.
A chromatographic assay of neomycin sulfate powders on strongly alkaline ion-exchange resin (hydroxide form) is described. D-(+)-alpha,alpha-Trehalose was used as an internal standard. The amount of neomycin B and C in commercial samples was determined with the proposed method, and the results are compared with those obtained by microbiological assay. In addition, minor neomycin components were estimated by TLC and GLC methods.
D001419:Bacteria / Q000187:drug effects; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005601:Framycetin / Q000031:analogs & derivatives* / Q000032:analysis*; D009355:Neomycin / Q000032:analysis* / Q000494:pharmacology; D011208:Powders / Q000032:analysis; D012116:Resins, Plant; D013464:Sulfuric Acids / Q000032:analysis
D011208:Powders; D012116:Resins, Plant; D013464:Sulfuric Acids; D005601:Framycetin; C031332:neomycin C; D009355:Neomycin