Determination of isoetharine in plasma by reversed-phase chromatography with amperometric detection.
A reversed-phase liquid chromatographic method for the determination of isoetharine in blood plasma, utilizing amperometric detection, is described. Plasma samples were extracted utilizing an ion-pair reagent, di-(2-ethylhexyl)phosphoric acid, to concentrate the catecholamine. Only minor differences were observed in the relative bioavailability of isoetharine hydrochloride and isoetharine mesylate after oral administration to rats. Observed plasma levels, at 1 hr after oral medication, were highly variable in dose-ranging studies at doses of 800-2500 mg/kg/day for 2 weeks.
D000284:Administration, Oral; D000605:Amino Alcohols / Q000097:blood*; D000818:Animals; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D007528:Isoetharine / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000605:Amino Alcohols; D007528:Isoetharine