The Z-band: 85,000-dalton amorphin and alpha-actinin and their relation to structure.
The conclusions arrived at as a result of this work can be summarized as follows: (a) We have found that there is an 85,000-dalton protein, which we have called 85K amorphin, associated with the Z-band of chicken pectoralis muscle myofibrils. We have isolated and purified this protein. It is not a structural component of the Z-filaments since it can be extracted completely without extraction of the Z-filaments. Extraction of 85K amorphin results in loss of specific staining of the Z-band with fluorescence specific anti-85K amorphin. (b) We have found that alpha-actinin is the structural component of the Z-filaments, since extraction of alpha-actinin is accompanied by loss of the Z-filament structure.
D000185:Actinin / Q000276:immunology / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000918:Antibody Specificity; D002645:Chickens; D008970:Molecular Weight; D009124:Muscle Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism*; D009132:Muscles / Q000648:ultrastructure*; D012519:Sarcoplasmic Reticulum / Q000032:analysis
D000596:Amino Acids; D009124:Muscle Proteins; C035770:Phosphorylase b protein, Gallus gallus; D000185:Actinin