Glutathione and non-protein sulfhydryl in cerebral cortex and lung in mice exposed to high oxygen pressure.
Mice were exposed to 6 atm of 100% O2, and killed at the onset of hyperactivity, convulsions, and 10(s) post convulsions. Examination of brain cortex from mice killed at these stages of O2 toxicity revealed no change in oxidized glutathione (GSSG), non-protein sulfhydryls (NPSH), total glutathione (GSH + GSSG), the GSH/GSSG ratio, glutathione reductase and glutathione peroxidase. Mice exposed to 4 atm for 1 h or 6 atm for 16 min exhibited a 36% and 33% decrease in lung NPSH respectively, but no change in cortical NPSH was observed. Although intraventricular diethylmaleate (DEM) decreased cerebral NPSH 72%, no change in the susceptibility of mice to O2 convulsions was found. Disulfiram, an effective O2 convulsive protectant had no effect on either cortical NPSH or total glutathione.
D000818:Animals; D001921:Brain / Q000187:drug effects; D002540:Cerebral Cortex / Q000032:analysis*; D005978:Glutathione / Q000032:analysis*; D008054:Lipid Peroxides / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010100:Oxygen / Q000633:toxicity*; D013438:Sulfhydryl Compounds / Q000032:analysis*
D008054:Lipid Peroxides; D013438:Sulfhydryl Compounds; D005978:Glutathione; D010100:Oxygen