Use of human erythrocyte ghosts for transfer of 125I-BSA and 125I-DNA into animal cells through cell fusion.
125I-BSA and human 125I-DNA fragments have been prepared for tests for loading efficiency by the erythrocyte ghosts, which is found to be 3.7 and 3.0 per cent respectively. The experiments are reproducible. The 125I-DNA fragments trapped in the erythrocyte ghosts are transferred into DON cells through cell fusion or "microinjection". The injection efficiency appraised by autoradiography amounts to 58 per cent.
D000818:Animals; D002459:Cell Fusion; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007457:Iodine Radioisotopes; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D012711:Serum Albumin, Radio-Iodinated
D007457:Iodine Radioisotopes; D012711:Serum Albumin, Radio-Iodinated; D012710:Serum Albumin, Bovine; D004247:DNA