Ageing in vivo does not alter the kinetics of DNA strand break repair.
The alkaline elution technique has been used to measure the apparent rate constant for repair of DNA strand breaks induced by X-radiation in human transformed lymphocyte DNA. Repair follows first-order kinetics and is essentially complete within 60 min. Although biological variation is observed there is no significant change in rate of repair with age of lymphocyte donor. In light of this it is unlikely that age-associated changes in DNA which have been widely observed arise from a changed persistence of strand breaks.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging; D000818:Animals; D002471:Cell Transformation, Neoplastic; D002648:Child; D002675:Child, Preschool; D004260:DNA Repair / Q000528:radiation effects; D004273:DNA, Neoplasm / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007223:Infant; D007231:Infant, Newborn; D007700:Kinetics; D007939:Leukemia L1210 / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects; D051379:Mice; D008875:Middle Aged
D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded