Automated high-performance liqiud chromatographic method for the determination of antipyrine and its metabolites in urine. Some preliminary results obtained from smokers and non-smokers.
A simple, accurate and fully automated high-performance liquid-chromatographic method was developed for the simultaneous determination of antipyrine (AP), 3-hydroxymethylantipyrine (3HMA), 4-hydroxyantipyrine (4OHA) and norantipyrine (NORA) in urine. This method requires no extraction step and only one chromatographic run with the use of a reversed-phase system. The coefficient of variation (%) (n = 8 each) was: 4.14 for AP, 2.31 for 3HMA, 3.48 for 4OHA, and 2.71 for NORA. The method was applied to studies on AP metabolism in three smokers and three non-smokers who received an oral 10 mg/kg dose of AP. These preliminary results suggest that smokers appear to excrete more 4OHA and NORA in the urine than non-smokers.
D000328:Adult; D000983:Antipyrine / Q000031:analogs & derivatives / Q000652:urine*; D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000077553:Edaravone; D005260:Female; D006801:Humans; D008297:Male; D012907:Smoking
C007983:4-hydroxyantipyrine; C031820:3-hydroxymethylantipyrine; D000077553:Edaravone; D000983:Antipyrine