The effect of lyophilization on steroid receptor behavior, as defined by sucrose density gradient analysis.
The effects of lyophilization of both cytosol and tissue powder were examined by sucrose density gradient analysis (SDGA) of uterine estrogen receptor (ER) and progesterone receptor (PgR) from pig and calf. Cytosol prepared from lyophilized material (both cytosol and tissue powder) was compared to that prepared concomitantly from frozen, pulverized powders of the same tissue. It was found that both ER and PgR responses to SDG ionic strength and the ability of ER to demonstrate temperature-dependent 4S leads to 5S transformation were unimpaired by lyophilization. In addition, lyophilized cytosol and lyophilized tissue powder gave equivalent results. A few experiments examining lyophilization effects on steroid receptors from human breast biopsies did note some diminution of the 8S form of both ER and PgR following lyophilization, although this effect was variable in extent. It is nevertheless concluded that much lyophilized material exhibits many of the same responses as frozen, and may be used in lieu of the latter, at least within the confines of the parameters described here.
D000818:Animals; D001943:Breast Neoplasms / Q000032:analysis; D002417:Cattle; D002499:Centrifugation, Density Gradient; D005260:Female; D005612:Freeze Drying; D006801:Humans; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis; D013048:Specimen Handling / Q000379:methods*; D013552:Swine; D013696:Temperature; D014599:Uterus / Q000032:analysis
D011960:Receptors, Estrogen; D011980:Receptors, Progesterone