[Isolation and translation of late mRNA coding for the structural proteins of simian adenovirus SA7].
A total preparation of cytoplasmic RNA was isolated from late stage-infected African green monkey kidney cells using phenol extraction procedure. The poly (A)-containing mRNA fraction was selected on oligo(dT)-cellulose columns. The resulting mRNA preparations were heterogenous in size and contained about 20--60% of SA7-derived sequences. SA7 late mRNA was efficiently translated in rabbit reticulocyte cell-free system giving rise to a number of polypeptide products that were related by antigenicity to authentic SA7 virion proteins. The main translation product having a molecular weight of 114 kilodaltons was identified as intact SA7 hexon protein.
D000256:Adenoviridae / Q000235:genetics*; D000261:Adenoviruses, Simian / Q000235:genetics*; D000818:Animals; D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D002474:Cell-Free System; D002522:Chlorocebus aethiops; D005814:Genes, Viral; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D011817:Rabbits; D014764:Viral Proteins / Q000235:genetics*; D014776:Virus Cultivation
D036022:Capsid Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; C018315:hexon capsid protein, Adenovirus
Vydelenie i transliatsiia pozdnikh mRNK, kodiruiushchikh strukturnye belki adenovirusa obez'ian SA7.