Effects of domperidone on the secretion of prolactin from rat anterior pituitary glands.
The effects of omperidone on prolactin secretion from the anterior pituitary glands of female rats were studied. A perifusion system and radioimmunoassay were used to study secretion; static incubations, and incorporation of [3H]leucine were used to measure biosynthesis. During perifusion, intact anterior lobes showed a constant rate of prolactin secretion for up to 5h (after a 90-min preincubation to stabilize the tissue). Incorporation studies revealed an increase in protein synthesis in perifused hemipituitary glands. When glands were treated with 10nM-domperidone , prolactin secretion began to decline after the first hour, reaching a maximum of 40-50% inhibition after a further 90 min. Growth hormone secretion showed no such decline. Inhibition of prolactin secretion continued for up to 2h after withdrawal of the drug. Treatment with 100nM-dopamine resuled in a more rapid inhibition of secretion, but the effect was reversed on withdrawal of the catecholamine. The prolactin content of perifused hemipituitary glands was measured after treatment with domperidone; the contents of control and treated glands did not differ, but were depleted compared with hemipituitary glands which had not been perifused.
D000818:Animals; D004294:Domperidone / Q000494:pharmacology*; D005260:Female; D013006:Growth Hormone / Q000378:metabolism; D066298:In Vitro Techniques; D010477:Perfusion; D010903:Pituitary Gland, Anterior / Q000187:drug effects* / Q000378:metabolism; D011388:Prolactin / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D004294:Domperidone; D011388:Prolactin; D013006:Growth Hormone