Reactivity of fibrinogen derivatives with antisera to human fibrin D-dimer and its gamma-gamma chain remnant.
Highly purified D-dimer was obtained from plasmin digest of human cross-linked fibrin. After reduction of its disulfide bonds, the gamma-gamma chain remnant, containing cross-linking site, was then isolated by ion-exchange chromatography on CM-cellulose. Antisera obtained by immunizing rabbits with D-dimer and its gamma-gamma chain remnant contained a small population of antibodies which specifically reacted with D-dimer. Thus, a specific radioimmunoassay system allowing detection and quantitation of D-dimer in the presence of fibrinogen and monomeric fragment D was made possible.
D000818:Animals; D000918:Antibody Specificity; D001667:Binding, Competitive; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D011817:Rabbits; D011863:Radioimmunoassay
D003432:Cross-Linking Reagents; D005338:Fibrin Fibrinogen Degradation Products; D007106:Immune Sera; C015214:fibrinogen D fragment