[Production and characterization of antisera against O,O-diethyl-O-(p-nitrophenyl)-phosphate (paraoxon)].
Methods for the production of antibodies against Paraoxon in rabbits are described. The highest-titre antisera were produced with conjugates containing a succinyl spacer group with a degree of derivatisation between 8 and 12 haptens per albumin molecule. The antibody response was tested by immunoprecipitation in agar gel with a hapten-rabbit-albumin-complex and by a radioimmunoassay. The synthesis of 125I labelled tracers is reported. The most potent antisera bound 50% of the radioactive tracer after 1:10(5) dilution and showed apparent affinity constants of 10(6) to 10(7) M-1 for Paraoxon. Compared to Paraoxon these antisera possessed cross reactivities with Parathion of 8.8 to 13.8%, with Methylparathion of 0.35 to 0.82%, and with Dimethoat of 0.0006 to 0.0012%.
D000818:Animals; D000917:Antibody Formation; D001345:Autoradiography; D055598:Chemical Phenomena; D002621:Chemistry; D006241:Haptens / Q000276:immunology; D007106:Immune Sera / Q000276:immunology*; D010261:Paraoxon / Q000276:immunology*; D011817:Rabbits
D006241:Haptens; D007106:Immune Sera; D010261:Paraoxon
Erzeugung und Charakterisierung von Antiseren gegen O,O-Diäthyl-O-(p-nitrophenyl)-phosphat (Paraoxon).