Identification and measurement of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline by gas chromatography-mass spectrometry.
A glass capillary gas chromatographic-mass spectrometric method for the analysis of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline in urine, plasma and blood platelets was developed. The method involves the use of a deuterated analogue as internal standard, addition of semicarbazide, extraction with methylene chloride and formation of pentafluoropropionyl derivatives. The 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline was found to occur in the urine of controls and alcoholics as both the free and conjugated compound. In the controls the mean level of free and conjugated 60MTHBC was 75 +/- 20 nmole/l. When intoxicated, the alcoholics had a statistically higher excretion rate of the conjugate(s) than the controls. The compound was not detectable in blood platelets or plasma.
D000328:Adult; D000437:Alcoholism / Q000097:blood / Q000652:urine; D001792:Blood Platelets / Q000032:analysis; D002243:Carbolines / Q000032:analysis* / Q000097:blood / Q000652:urine; D055598:Chemical Phenomena; D002621:Chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D007211:Indoles / Q000032:analysis*; D008297:Male; D008875:Middle Aged
D002243:Carbolines; D007211:Indoles; C035602:6-hydroxy-1-methyltryptoline