The binding of prednisolone in human serum and to recrystallized human albumin in vitro.
1 Prednisolone binding in human serum and to recrystallized human serum albumin was measured by continuous ultrafiltration. 2 At serum concentrations of prednisolone up to 0.6 micron, 95.0% was bound but at higher concentrations the binding became non-linear falling to 80.5% at 1.8 microns. At even higher concentrations binding in serum became linear again and paralleled the binding to albumin which was linear throughout the entire range of prednisolone concentrations. 3 A binding model was proposed which included a saturable component attributable to binding to transcortin and a non-saturable binding to albumin. 4 Computer simulations using the experimentally determined binding parameters of the model indicated that binding in serum was critically dependent on transcortin concentration and almost independent of albumin concentration.
D000328:Adult; D003460:Crystallization; D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D066298:In Vitro Techniques; D008954:Models, Biological; D011239:Prednisolone / Q000097:blood*; D011485:Protein Binding; D012709:Serum Albumin / Q000032:analysis*; D014461:Ultracentrifugation
D012709:Serum Albumin; D011239:Prednisolone