Recombinant DNA methods in neuroendocrinology: new answers to old questions.
The development of methods for cloning DNA complementary to specific messenger RNAs encoding polypeptide hormones has opened new experimental avenues in the neurosciences. Recombinant DNA techniques have been used to answer questions about the structure of polypeptide hormone precursors, the regulation of peptide hormone production, and the location of peptide-producing cells in nervous and endocrine systems. Here, the application of cDNA cloning to the quantitation, structural elucidation and tissue localization of mRNAs coding for neuroendocrine peptides is reviewed.
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004702:Endocrine Glands / Q000378:metabolism*; D006728:Hormones / Q000096:biosynthesis*; D006801:Humans; D009420:Nervous System / Q000378:metabolism*; D010452:Peptide Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism
D004274:DNA, Recombinant; D006728:Hormones; D012333:RNA, Messenger