[Reaction of cells to hypertonic effects by cryoprotection].
Kinetics of volume changes in cells caused by hypertonic solutions has been investigated theoretically using an apparatus of the non-equilibrium thermodynamics. Analytical expressions for relaxation times of the two-stage process studied have been found. The reaction of mouse peritoneal exudate cells to hypertonic solutions of glycerol. PEO-400 or PEO-1500 has been studied experimentally. An agreement has been obtained between theoretical calculations and experimental results. Possible mechanism of cell injury caused by hypertony is discussed.
D000818:Animals; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology; D007700:Kinetics; D008264:Macrophages / Q000502:physiology*; D008433:Mathematics; D051379:Mice; D009994:Osmolar Concentration; D011092:Polyethylene Glycols / Q000494:pharmacology; D013816:Thermodynamics
D011092:Polyethylene Glycols; D005990:Glycerol
Reaktsiia kletok na gipertonicheskie vozdeĭstviia pri krioprotektsii.