A polycarbonate filter technique for collection of sorted cells.
We describe the application of polycarbonate filters to the collection of cells sorted by flow cytometry. Cells are sorted directly onto polycarbonate filters and transferred to microscope slides which are then processed to dissolve the filter and remove pore outlines. The technique results in cell preparations which retain cellular morphology and have high cell recovery of 82-100%.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D005374:Filtration; D005434:Flow Cytometry / Q000379:methods*; D007421:Intestine, Small / Q000166:cytology; D051379:Mice; D011076:Polycarboxylate Cement; D012513:Sarcoma, Experimental / Q000473:pathology
D011076:Polycarboxylate Cement; C005506:polycarbonate