[Insensitivity of purified glutathione reductase from adrenal cortex to o,p'-dichlorodiphenyldichloroethane].
The activity of partially purified glutathione reductase (EC 1.6.4.2) was studied as affected by 1-(o-chlorophenyl)-1-(p-chlorophenyl)-2,2-dichloroethane (o,p'-DDD, chloditane). The glutathione reductase from human and dog adrenal cortex was purified by means of precipitation with (NH4)2SO4, by affinity chromatography, sephadex G25, DEAE-cellulose and CM-cellulose chromatography. A 56.6- and 41-fold purification was obtained for the enzyme from human and dog adrenal cortex, respectively. o,p'-DDD did not influence the activity of purified glutathione reductase in vitro. The rate of NADPH oxidation by adrenal cortex cytosol in the glutathione reductase reaction increased under the effect of o,p'-DDD.
D000302:Adrenal Cortex / Q000201:enzymology*; D000818:Animals; D003600:Cytosol / Q000201:enzymology; D004285:Dogs; D005980:Glutathione Reductase / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008939:Mitotane / Q000494:pharmacology*
D008939:Mitotane; D005980:Glutathione Reductase
Nechuvstvitel'nost' ochishchennoĭ glutationreduktazy kory nadpochechnikov k o,p'-dikhlordifenildikhlorétanu.