New reagents for the introduction of the thiomethyl group at sulfhydryl residues of proteins with concomitant spectrophotometric titration of the sulfhydryls: methyl 3-nitro-2-pyridyl disulfide and methyl 2-pyridyl disulfide.
D055598:Chemical Phenomena; D002621:Chemistry; D008745:Methylation; D010455:Peptides; D011506:Proteins; D011725:Pyridines; D013056:Spectrophotometry, Ultraviolet; D013438:Sulfhydryl Compounds / Q000032:analysis*; D013439:Sulfhydryl Reagents
D010455:Peptides; D011506:Proteins; D011725:Pyridines; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; C035119:methyl 2-pyridyl disulfide; C035118:3-nitro-2-pyridyl disulfide