Hyperbaric ethanol antagonism in mice: time course.
C57BL/6J mice were injected intraperitoneally with 3.6 g/kg ethanol and exposed to 1 ATA air or to 1 or 12 ATA helium-oxygen immediately, 5, 10 or 20 minutes after injection. Immediate and delayed hyperbaric exposure produced similar significant reductions in sleep-times and increases in wake-up brain ethanol concentrations. These findings demonstrate the effectiveness of delayed hyperbaric treatment in reducing ethanol sleep-time and eliminate decreased absorption and altered distribution as critical factors mediating the antagonism. The results are consistent with current theories of anesthesia and support a direct mechanism involving brain cellular membranes.
D000818:Animals; D001921:Brain / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D000431:Ethanol / Q000037:antagonists & inhibitors*; D006371:Helium / Q000494:pharmacology; D006931:Hyperbaric Oxygenation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000494:pharmacology; D012890:Sleep / Q000187:drug effects*; D013997:Time Factors
D006371:Helium; D000431:Ethanol; D010100:Oxygen