Basement membrane glycoproteins in the extracellular matrix of a teratocarcinoma-derived differentiated cell line.
Glycoproteins synthesized and deposited into extracellular matrix in cultures of a mouse teratocarcinoma-derived differentiated cell line (PYS-2) were studied by metabolic labeling and immunochemical methods, and by immunofluorescence and electron microscopy. PYS-2 cells synthesized two major high-molecular weight glycoproteins, laminin and type IV collagen, which were deposited in matrix form with no apparent processing. The lamellar subcellular matrix was composed of a loose network of fine fibrils and dense grains. Straight 60-80 nm fibrils attached the matrix to the plasma membranes and were decorated with ferritin-coupled antibodies for laminin. The cells did not synthesize fibronectin, but the matrix bound fibronectin from the culture medium. The PSY-2 matrix provides possibilities for in vitro studies on basal lamina components in early differentiation.
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006651:Histocytochemistry; D007797:Laminin; D051379:Mice; D008854:Microscopy, Electron; D013724:Teratoma / Q000378:metabolism*
D005353:Fibronectins; D006023:Glycoproteins; D007797:Laminin; D003094:Collagen