Interaction of a human plasma lipid transfer protein complex with lipid monolayers.
The interaction of a purified human plasma lipid transfer complex with cholesteryl ester, triacylglycerol and phosphatidylcholine in binary and ternary lipid monolayers was investigated. The lipid transfer complex, designated LTC, catalyzes the removal of cholesteryl oleate and triacylglycerol from phosphatidylcholine monolayers. Preincubation of LTC with p-chloromercuriphenyl sulfonate inhibits LTC-catalyzed removal of triacylglycerol; cholesteryl ester removal is not affected. The rate of LTC-facilitated removal of cholesteryl oleate from a phosphatidylcholine monolayer depends on the amount of LTC added to the subphase up to 100 micrograms protein. In addition, the rate of the LTC-catalyzed transfer of cholesteryl oleate to the subphase increases linearly as the amount of cholesteryl oleate in the monolayer increases to 6 mol%. LTC also removes cholesterol from phosphatidylcholine-cholesterol monolayers, albeit at a rate which is 15% of that for removal of cholesteryl oleate. The ability of LTC to facilitate triacylglycerol and cholesteryl ester removal depends on the composition of the monolayer. Phosphatidylcholine supports cholesteryl ester transfer whereas sphingomyelin-cholesteryl ester monolayers are almost refractory to LTC. In contrast, LTC removes triacylglycerol from either a phosphatidylcholine or a sphingomyelin monolayer. The results suggest the existence of at least two lipid transfer proteins, one of which catalyzes the removal of cholesteryl ester and the other triacylglycerol. The role of these proteins as they relate to lipoprotein metabolism is discussed.
D002352:Carrier Proteins / Q000097:blood* / Q000302:isolation & purification; D002788:Cholesterol Esters / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
D002352:Carrier Proteins; D002788:Cholesterol Esters; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D014280:Triglycerides; C030363:lipid transfer protein