Inhibition of anaerobic glycolysis in bovine retina extracts by salicylate and acetylsalicylate.
1. Na salicylate 31 mM inhibits anaerobic glycolysis from glucose in bovine retina extracts. The formation rate of DAP and GAP increases while that of FDP, G6P, F6P and lactate decreases. All the above modifications are almost completely removed by 1.4 mM NAD+. 2. Bovine retina extracts, preincubated for 1 hr at 0 degrees C with 31 mM Na salicylate show a strongly reduced glycolytic activity. In this system G6P and F6P do accumulate, FDP, DAP, GAP and lactate decrease. These effects are not altered adding 3.5 mM NAD+ to the preincubation mixture. 3. Acetylsalicylate 31 mM inhibits anaerobic glycolysis in crude retina extracts. As the rate of lactate formation decreases, G6P and F6P do accumulate, while FDP, DAP and GAP diminish. 4. Identical modifications are observed adding the inhibitor directly to the incubation mixture, or preincubating it with the extracts at 0 degrees C for 4 hr. 3.5 mM NAD+ does not remove the effects of acetylsalicylate.
D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D002417:Cattle; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects*; D066298:In Vitro Techniques; D007773:Lactates / Q000096:biosynthesis; D019344:Lactic Acid; D010100:Oxygen; D012160:Retina / Q000187:drug effects* / Q000378:metabolism; D012459:Salicylates / Q000494:pharmacology*; D020156:Salicylic Acid
D007773:Lactates; D012459:Salicylates; D019344:Lactic Acid; D005947:Glucose; D020156:Salicylic Acid; D001241:Aspirin; D010100:Oxygen