Interlaboratory survey of enzyme analyses IV. Human versus porcine tissue as source of creatine kinase for survey serum.
A special enzyme survey was carried out in 185 laboratories on specimens fortified with either porcine heart or human skeletal muscle creatine kinase (CK). All analytical systems were examined to see if they gave different results for porcine and human CK as compared with analysis of the same specimens on the duPont ACA. Analytical differences were found. However, these differences were small, and, with some exceptions, do not prevent interconversion of units based solely on specimens fortified with porcine CK. Both types of specimens serve equally well in estimating bias and variability of CK analyses. Based on the results for the human material, many laboratories appear to use inappropriate reference ranges for CK.
D000818:Animals; D003201:Computers; D003402:Creatine Kinase / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D008722:Methods; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D012016:Reference Values; D013552:Swine
D007202:Indicators and Reagents; D003402:Creatine Kinase