Molecular sieve chromatography of some plasma protein products by high performance liquid chromatography: comparison of quantitation by UV absorption and Lowry protein.
Commercially prepared fraction V products (5 and 25% albumin and 5% plasma protein fraction) and immune serum globulins were subjected to analytical molecular sieve chromatography by high performance liquid chromatography. Quantitation was performed by UV absorbance and by the Lowry protein assay. The proteins eluting in the void volume from the immune serum globulin samples yield equivalent results by UV absorbance and by Lowry assay. Proteins eluting in the void volume from fraction V products indicate approximately twice as much protein by UV absorbance when compared to Lowry protein assay.
D001798:Blood Proteins / Q000032:analysis*; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D008970:Molecular Weight; D013056:Spectrophotometry, Ultraviolet; D014461:Ultracentrifugation
D001798:Blood Proteins; D007136:Immunoglobulins