title: [Persistence of Newcastle disease virus in mouse cells].
abstract: The course of persistent infection in Newcastle disease virus (NDV)-infected mouse L cells (LNDV) was analysed. The production of infectious intra- and extracellular virus was markedly reduced (less than 10(1) PFU/ml), 30% of the cells produced virus-specific antigen. Analysis of the synthesis of 3H-nucleocapsid RNA in the LNDV system revealed predominance of low molecular weight RNAs. Virus-specific sequences were shown to be present in fraction of DNA extracted by Hirt's method. Only the "supernatant" Hirt's fraction was infectious. The possibility of the presence of virus-specific sequences in free unintegrated form is discussed.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D004279:DNA, Viral / Q000302:isolation & purification; D007739:L Cells; D051379:Mice; D009522:Newcastle disease virus / Q000254:growth & development* / Q000276:immunology; D012367:RNA, Viral / Q000302:isolation & purification; D010948:Viral Plaque Assay
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D012367:RNA, Viral
vernacular_title: Persistentsiia virusa bolezni N'iukasla v myshinykh kletkakh.