[Radio-immuno-assay for trypsin in newborn-screening for cystic fibrosis].
In 4,956 infants the concentration of immunoreactive trypsin was measured in dried blood on filter paper using a double antibody radio immuno assay. About 90% of all results were below 40 ng/ml. In 13 infants the concentration of immunoreactive trypsin exceeded 80 ng/ml. These infants were examined clinically, including sweat iontophoresis. We found three children suffering from cystic fibrosis. One further child showing an elevated concentration of chloride in the sweat (60 mval/ml) could not be reexamined. The concentration of immunoreactive trypsin in the cystic fibrosis children was 230, 297, and in one case 108 ng/ml at the 76th day of life, whereas the values for the 9 other children were between 80 and 154 ng/ml. We believe these results justify to use this test for a much higher number of infants, especially because it is inexpensive and can easily be added to existing newborn screening programs for inborn errors of metabolism.
D003550:Cystic Fibrosis / Q000097:blood*; D006801:Humans; D007231:Infant, Newborn; D011863:Radioimmunoassay; D012016:Reference Values; D013997:Time Factors; D014357:Trypsin / Q000097:blood*; D014362:Trypsinogen / Q000097:blood
D014362:Trypsinogen; D014357:Trypsin
Radio-Immuno-Assay auf Trypsinogen für ein Neugeborenen-Screening auf Mukoviszidose.