In vitro crystallization of ribosomes from chick embryos.
A new two-dimensional ribosome crystal, having the tetragonal space group P42(1)2 (a = 593 A), has been grown from ribosome tetramers extracted from hypothermic chick embryos. It is of particular interest because of its larger size (up to 3 x 3 micron2) and greater stability compared to other related polymorphic forms, and because it can easily be grown in large amounts. X-ray diffraction shows the order in the crystal to extend to a resolution of at least 60 A. The crystalline ribosomes appear to contain a full complement of small and large ribosomal subunit proteins and an additional four proteins not characteristic of chick embryo polysomes.
D000818:Animals; D001224:Aspartic Acid; D002642:Chick Embryo; D003460:Crystallization; D003956:Dialysis; D005583:Fourier Analysis; D006863:Hydrogen-Ion Concentration; D012269:Ribosomal Proteins / Q000032:analysis; D012270:Ribosomes / Q000032:analysis / Q000648:ultrastructure*; D013096:Spermine; D014961:X-Ray Diffraction
D012269:Ribosomal Proteins; D013096:Spermine; D001224:Aspartic Acid