[Rapid visualization and separation of serum lipoproteins of the rat by ultracentrifugation in saline density gradients].
A method is presented for the visualization and location of lipoprotein bands in density gradient ultracentrifugation. A 3 hour spin results in a good separation of the major rat serum lipoprotein classes: very low, low and high density lipoproteins, by using small centrifuge tubes and highspeed rotors. In addition, the concentration of cholesterol, triglyceride, phospholipid and protein in the lipoprotein fractions obtained by this technique was also determined.
D000818:Animals; D001391:Azo Compounds; D002499:Centrifugation, Density Gradient; D002784:Cholesterol / Q000097:blood; D008055:Lipids / Q000097:blood; D008074:Lipoproteins / Q000097:blood* / Q000302:isolation & purification; D008297:Male; D009281:Naphthalenes; D010750:Phosphoproteins / Q000097:blood; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000097:blood
D001391:Azo Compounds; D008055:Lipids; D008074:Lipoproteins; D009281:Naphthalenes; D010750:Phosphoproteins; D014280:Triglycerides; D002784:Cholesterol; C016118:Sudan Black B
Visualización y separación rápida de las lipoproteínas séricas de la rata por ultracentrifugación en gradientes salinos de densidad.