Evidence for a compartmentation of brain microsomal diacylglycerol.
Phosphatidylcholine synthesis from CDP-[methyl-14C]choline and membrane-bound diacyl-[U-14C]-sn-glycerol, formed through the glycerol phosphate pathway, has been examined in vitro in rat brain microsomes. When labeled diacylglycerol was incubated in the presence of unlabeled CDP-choline, the rate of phospholipid labeling looked very different from that measured in incubations of unlabeled diacylglycerol with CDP-[methyl-14C]choline. Evidence is given that diacylglycerol formed through the glycerol phosphate pathway belongs to a metabolic pool separate from the bulk membrane diacylglycerol.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002250:Carbon Radioisotopes; D003566:Cytidine Diphosphate Choline / Q000378:metabolism; D003568:Cytidine Monophosphate / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism*; D005989:Glycerides / Q000378:metabolism*; D007700:Kinetics; D008861:Microsomes / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000096:biosynthesis; D010740:Phospholipases; D051381:Rats
D002250:Carbon Radioisotopes; D004075:Diglycerides; D005989:Glycerides; D010713:Phosphatidylcholines; D003566:Cytidine Diphosphate Choline; D010740:Phospholipases; D003568:Cytidine Monophosphate