title: A sixth species of ovine adenovirus isolated from lambs in New Zealand.
abstract: Two adenoviruses (WV419/75 and WV757/75), isolated from lambs in New Zealand were compared using neutralisation tests with the five recognised ovine adenovirus species, NINE bovine and four porcine adenoviruses. WV419/75 did not cross-react with any of the viruses tested and represents a new ovine adenovirus species (OAV-6). WV757/75 cross-reacted with bovine adenovirus type 7 (BAV-7) with a homologous to heterologous titre ratio of 16 in one direction only, and also showed a substantial one-way cross reaction in haemagglutination-inhibition tests (WV757 antiserum inhibiting haemagglutination by BAV-7). There was therefore insufficient distinction from BAV-7 virus to allow designation as a separate species. Fluorescent antibody studies with WV419 and WV757 demonstrated virus inclusions in the nuclei of infected cells. These were stained by antiserum to OAV-4 indicating presence of the mammalian group antigen. Thin section electron microscope studies showed typical adenovirus particles and associated inclusions in cell nuclei. The similarity of the two viruses to the bovine subgroup 2 adenoviruses in several of their properties is discussed.
mesh_terms: D000256:Adenoviridae / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D000956:Antigens, Viral; D003429:Cross Reactions; D006385:Hemagglutination Inhibition Tests; D009520:New Zealand; D012756:Sheep / Q000382:microbiology*; D013045:Species Specificity
chemical_list: D000956:Antigens, Viral