Transport of methionine by normal and transformed rat embryo fibroblasts.
Methionine uptake was 2-2.3-fold higher in the transformed rat embryo fibroblasts as compared with the non-transformed cells. This was associated with a 2-fold greater Vmax, with no detectable changes in Km values. Methionine starvation caused an increase in the rate of transport, the increase being higher in the transformed cells. Addition of methionine abolished the starvation effect, although to a smaller extent in the transformed cells. It is suggested that differences in methionine uptake might be due to a quicker depletion of intracellular methionine, and not to changes in the transport system of the transformed cells.
D000818:Animals; D000831:Animals, Newborn; D001692:Biological Transport; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D007700:Kinetics; D008715:Methionine / Q000378:metabolism*; D011247:Pregnancy; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D011506:Proteins; D008715:Methionine