Distribution of newly synthesized DT-diaphorase in rat liver.
The distribution, synthesis, transport, and glycosylation of rat-liver DT-diaphorase has been investigated. The enzyme could be isolated using specific antibodies, mainly from the soluble supernatant but also from microsomal vesicles, Golgi membranes, and mitochondria. 40% of the microsomal enzyme was located in the lumen or on the interior side of the membrane, the rest remaining as an integral non-extractable part of the membrane. Synthesis of DT-diaphorase takes place on both free and bound ribosomes, although it was found to be transported in a sequential manner from the rough to the smooth endoplasmic reticulum and also subsequently to the mitochondria. The rough and smooth microsomal DT-diaphorase contains covalently bound carbohydrate, but no sugar moiety could be detected bound to the cytoplasmic form of the enzyme.
D000818:Animals; D006056:Golgi Apparatus / Q000201:enzymology; D007425:Intracellular Membranes / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D009247:NADH, NADPH Oxidoreductases / Q000096:biosynthesis*; D011808:Quinone Reductases / Q000096:biosynthesis* / Q000302:isolation & purification; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology
D009247:NADH, NADPH Oxidoreductases; D011808:Quinone Reductases