title: Histamine contamination of pokeweed mitogen.
abstract: The histamine content of commercial preparations of pokeweed mitogen was measured by amino acid analysis technique, automated fluorometry, and bioassay employing the guinea pig ileum. Ten samples from 5 companies were examined and found to contain between 0.026 micrograms and 167.5 micrograms of histamine per ml of solution. The protein content of 9 of these putative 5 mg samples measured by folin assay and by amino acid analysis varied from 0.56 to 4.4 mg. Their amino acid compositions were similar, except for notable variations in 3 of the 16 residues quantitated.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004340:Drug Contamination; D005470:Fluorometry; D006168:Guinea Pigs; D006632:Histamine / Q000032:analysis*; D009130:Muscle, Smooth / Q000187:drug effects; D011043:Pokeweed Mitogens / Q000032:analysis*; D011506:Proteins / Q000032:analysis
chemical_list: D000596:Amino Acids; D011043:Pokeweed Mitogens; D011506:Proteins; D006632:Histamine