title: Blood volume of nonsplenectomized and splenectomized cats before and after acute hemorrhage.
abstract: Blood volume (BV) was determined in awake, nonsplenectomized (NSPX) and splenectomized (SPX) cats before and after hemorrhage (6 ml/kg). Each NSPX cat had a determined BV at least 10 ml/kg greater than the same cat after splenectomy. The mean BV of SPX cats was 43.4 +/- 8.94. ml kg (4.3% of body weight). The calculated RBC masses of NSPX and SPX cats were 17.0 +/- 4.07 and 12.2 +/- 1.12 ml/kg, respectively. Each NSPX cat had apparent RBC masses of 5 ml/kg greater than that of the same cat after splenectomy was done. At 1 hour after a hemorrhage, the BV and RBC masses determined in SPX cats were 46.7 +/- 12.1 and 9.7 +/- 1.90 ml/kg, respectively. Extravascular-to-intravascular fluid flux (calculated from RBC masses and plasma protein dilution) was approximately 0.80% of body weight. The indirect method with 51Cr-labeled RBC for BV determination was accurate and precise in awake, SPX cats; in awake, NSPX cats, the 51Cr-labeled RBC dilution method was precise, but not accurate. The spleen in the cat resulted in marked overestimations of BV and RBC masses.
mesh_terms: D000208:Acute Disease; D000818:Animals; D001810:Blood Volume; D001811:Blood Volume Determination / Q000379:methods / Q000662:veterinary; D002371:Cat Diseases / Q000503:physiopathology*; D002415:Cats / Q000502:physiology*; D002840:Chromates; D002860:Chromium Radioisotopes; D004911:Erythrocyte Volume; D005260:Female; D006470:Hemorrhage / Q000503:physiopathology / Q000662:veterinary*; D008297:Male; D017670:Sodium Compounds; D013154:Spleen / Q000502:physiology; D013156:Splenectomy / Q000662:veterinary*
chemical_list: D002840:Chromates; D002860:Chromium Radioisotopes; D017670:Sodium Compounds; C028982:sodium chromate(VI)