Alterations in glycopeptide and glycosaminoglycan biosynthesis in bovine lens epithelial cells cultured in the presence of an eye-derived growth factor.
D-[3H]glucosamine-labeled glycosaminoglycans and glycopeptides, obtained by Pronase digestion from bovine lens epithelial cells grown in the presence and in the absence of an eye-derived growth factor, were investigated comparatively in the extra- and pericellular compartments of 16-17th subcultures. Glycosaminoglycans and glycopeptides were separated on Ultrogel AcA 202 column and the retained glycopeptides were further separated by the successive application of affinity, ion-exchange and exclusion chromatographic methods. The following results were obtained: (1) the proportion of the label increased in hyaluronic acid and decreased in the glycopeptide fraction on stimulation with the growth factor: (2) the comparison of the chromatographic elution profiles of the glycopeptides from control and stimulated cells revealed that differences between the two cell groups were largely quantitative. An increase in the high molecular weight glycopeptides, as it was found in transformed cells, could not be demonstrated in eye-derived growth factor-treated cells.
D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002417:Cattle; D002478:Cells, Cultured; D004848:Epithelium / Q000378:metabolism; D005346:Fibroblast Growth Factors; D006020:Glycopeptides / Q000096:biosynthesis* / Q000302:isolation & purification; D006025:Glycosaminoglycans / Q000096:biosynthesis* / Q000302:isolation & purification; D006133:Growth Substances / Q000502:physiology*; D007908:Lens, Crystalline / Q000378:metabolism*; D008970:Molecular Weight
D002241:Carbohydrates; D006020:Glycopeptides; D006025:Glycosaminoglycans; D006133:Growth Substances; C039825:eye-derived growth factor; D005346:Fibroblast Growth Factors