beta-Fibrillary bodies in low-grade adenocarcinoma of parotid gland: a histochemical and ultrastructural study.
Rounded or polyhedral, acellular, dense lamellated structures were seen within the cystic and glandular spaces in a case of low-grade papillary adenocarcinoma of the parotid gland. These structures displayed the histochemical characteristics of amyloid, namely, pink color with apple-green birefringence and bright red fluorescence with alkaline congo red, orthochromasia and red birefringence with standardized toluidine blue, positive dimethylaminobenzaldehyde nitrite and diazotization coupling reactions, and spontaneous autofluorescence. At the ultrastructural level the tumor consisted of cuboid, flattened, or elongated epithelial cells with varying degrees of differentiation, but most mature tumor cells had numerous intracellular intermediate filaments. These filaments were randomly oriented and had no lateral fasciation; they were 10 nm wide and similar to those constituting the intraluminal corpora amylacea. These bodies displayed increased fibrillary density. Cellular organelles and pyknotic nuclei were frequently present at the periphery of the fibrillary masses. Amyloid masses, histochemically and ultrastructurally indistinguishable from those in the lumens, were present in the stroma as well. It is postulated that both stromal and intraluminal beta-fibrillary bodies result from apoptosis and desquamation of neoplastic cells, with progressive release and confluency of intracellular intermediate filaments within closed cystic spaces. This additional evidence of beta-fibrillosis associated with a slow-growing non-hormone-secreting salivary gland tumor suggests that these tumors may be associated with amyloidosis more frequently than was previously suspected.
D000231:Adenocarcinoma, Papillary / Q000032:analysis / Q000473:pathology / Q000601:surgery / Q000648:ultrastructure*; D000682:Amyloid / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010307:Parotid Neoplasms / Q000032:analysis / Q000473:pathology / Q000601:surgery / Q000648:ultrastructure*
D000682:Amyloid