Stable integration of adenovirus DNA is not required for the induction of mutations in the hypoxanthine phosphoribosyltransferase gene in Chinese hamster cells.
Adenovirus type 2 induces EMS-like mutations at the hpt13 locus coding for hypoxanthine phosphoribosyl transferase (HPRT) in Chinese hamster cells (J. Virol., 38, 184--190, 1981). In this report we show that: (a) the hpt- 13 mutation is in the structural gene of HPRT, (b) adenovirus induces intracistronic reversion mutations which suppress the hpt- 13 phenotype, and (c) the stable integration of adenovirus DNA is not required for the induction of these mutations.
D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D006224:Cricetinae; D003412:Cricetulus; D004279:DNA, Viral / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D009154:Mutation; D010641:Phenotype
D004279:DNA, Viral; D007041:Hypoxanthine Phosphoribosyltransferase