Synthesis of RNA segments 1-3 during generation of incomplete influenza A (fowl plague) virus.
Incomplete influenza A virus (fowl plague Dobson strain) was prepared by undiluted passage in primary chick embryo fibroblast cells. Analysis of released virus RNA revealed a deficiency in RNA segments 1-3, characteristic of incomplete virus formation. The virus yield from a high multiplicity infection with standard virus always showed this deficiency, even when analysed as early as 6 hours post-infection, whereas infection at low multiplicity gave rise to virus indistinguishable in RNA composition from the parent virus. The relative amounts of intracellular, non-polyadenylated, complementary RNA (template RNA) were found to reflect accurately the eventual RNA composition of released virus, and were altered in phase with PFU:HAU ratio, throughout a von Magnus cycle.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003673:Defective Viruses / Q000254:growth & development* / Q000378:metabolism; D009980:Influenza A virus / Q000254:growth & development* / Q000378:metabolism; D007700:Kinetics; D012367:RNA, Viral / Q000096:biosynthesis*; D013698:Templates, Genetic; D010948:Viral Plaque Assay
D012367:RNA, Viral