Mutation, DNA labeling, and transformation of BHK-21/CL 13 cells by MNNG, and nitrosocimetidine.
N-Methyl-N'-nitro-N-nitrosoguanidine (MNNG) has been reported to induce BHK-21/Cl 13 cell growth in agar suspension. To determine if MNNG was also mutagenic to BHK cells, an ouabain-resistance mutation assay was established using these cells. In this system MNNG was compared to nitrosocimetidine (NC). MNNG and NC did induce ouabain-resistant mutations in BHK cells. The ability of the test compounds to methylate DNA in BHK cells was also determined, and both MNNG and NC yielded detectable levels of 7-methylguanine in treated cells. MNNG and NC were tested for the ability to transform BHK cells, and did. NC was found to be as effective a mutagen and transforming agent in BHK cells as MNNG when administered at equitoxic concentrations; approx. 4-fold less effective at equimolar concentrations.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D002927:Cimetidine / Q000031:analogs & derivatives / Q000494:pharmacology*; D006224:Cricetinae; D004247:DNA / Q000378:metabolism*; D006146:Guanidines / Q000494:pharmacology*; D006147:Guanine / Q000031:analogs & derivatives / Q000096:biosynthesis; D007668:Kidney; D008647:Mesocricetus; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*
D006146:Guanidines; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D006147:Guanine; C008450:7-methylguanine; C019503:nitrosocimetidine; D002927:Cimetidine; D004247:DNA