Influence of pyridoxal-5'-phosphate on temperature relationship of aspartate aminotransferase and alanine aminotransferase.
The influence of pyridoxal-5'-phosphate on the temperature relationship of aspartate aminotransferase and alanine aminotransferase has been determined. On varying the reaction temperature from 25 degrees to 45 degrees both enzymes showed small but significant deviations from a linear Arrhenius relationship. Supplementation with pyridoxal-5'-phosphate in general enhanced the catalytic activity to the same degree over the entire temperature range. Our results corroborate the findings of Rej but not those of Jung et al.
D000410:Alanine Transaminase / Q000097:blood*; D001219:Aspartate Aminotransferases / Q000097:blood*; D006801:Humans; D007700:Kinetics; D011732:Pyridoxal Phosphate / Q000494:pharmacology*; D013696:Temperature; D013816:Thermodynamics
D011732:Pyridoxal Phosphate; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase