Radiochemical assay for gamma-aminolevulinate synthase.
We describe here a standard protocol for the radiochemical assay of gamma-aminolevulinate synthase from 17-day chick embryo liver mitochondria and starved male rat liver mitochondria. The assay is sensitive to at least 15 pmol of the gamma-aminolevulinate produced and can be adapted to suit most tissue types. A number of possible modifications to the procedure are discussed.
D000624:5-Aminolevulinate Synthetase / Q000032:analysis*; D000818:Animals; D002250:Carbon Radioisotopes; D002642:Chick Embryo; D002852:Chromatography, Ion Exchange; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology; D011506:Proteins / Q000378:metabolism; D051381:Rats; D013386:Succinates / Q000494:pharmacology; D019802:Succinic Acid
D002250:Carbon Radioisotopes; D011506:Proteins; D013386:Succinates; D019802:Succinic Acid; D000624:5-Aminolevulinate Synthetase