Determination of amygdalin and its major metabolite prunasin in plasma and urine by high pressure liquid chromatography.
A method is described for the determination of amygdalin and prunasin in plasma ultrafiltrate and urine. Both compounds are separated by high pressure liquid chromatography on a reversed phase column and subsequently detected at 215 nm. The identity of an amygdalin metabolite with prunasin was confirmed by mass spectrometry.
D000678:Amygdalin / Q000097:blood* / Q000652:urine; D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D013058:Mass Spectrometry; D009570:Nitriles / Q000097:blood* / Q000652:urine; D051381:Rats; D014462:Ultrafiltration
D009570:Nitriles; C019063:prunasin; D000678:Amygdalin