A quantitative antiglobulin test for IgG for use in blood transfusion serology.
A sensitive method has been developed using 125I-labelled anti-IgG which allows detection of IgG present on the red cell surface. By suitable absorption and correction for non-specific binding to trypsinized red cells, a reproducible, accurate method of quantitating IgG absorbed onto red cells is obtained. The number of IgG molecules present on normal cells was found to range from 5 to 90 with an average of 39. Studies of correlation of agglutination in the antiglobulin test with numbers of IgG molecules on the cell were also undertaken.
D001803:Blood Transfusion; D003298:Coombs Test / Q000379:methods*; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D007074:Immunoglobulin G / Q000032:analysis*; D007457:Iodine Radioisotopes; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D012699:Serology; D014357:Trypsin
D007106:Immune Sera; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D011947:Receptors, Antigen, B-Cell; D014357:Trypsin