Measurement of uroporphyrinogen decarboxylase using porphyrinogens prepared by chemical reduction.
A sensitive, specific method for the measurement of uroporphyrinogen decarboxylase(porphyrinogen carboxy-lyase, EC4.1.1.37) in liver homogenates, haemolysates and other tissues is described. Pentacarboxylic porphyrinogen III, prepared from the corresponding porphyrin by reduction with sodium-mercury amalgam, is used as substrate. After oxidation and methyl esterification the reaction product, coproporphyrinogen III, is separated from the substrate and measured by high-performance liquid chromatography, with mesoporphyrin IX as internal standard. The method can readily be adapted for use with any of the other substrates of uroporphyrinogen decarboxylase, including uroporphyrinogens I and III.
D000818:Animals; D002262:Carboxy-Lyases / Q000032:analysis*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003306:Coproporphyrins / Q000138:chemical synthesis; D005260:Female; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000378:metabolism; D010084:Oxidation-Reduction; D011165:Porphyrinogens / Q000138:chemical synthesis / Q000378:metabolism*; D011166:Porphyrins / Q000138:chemical synthesis; D051381:Rats; D011919:Rats, Inbred Strains; D014575:Uroporphyrinogen Decarboxylase / Q000032:analysis*
D003306:Coproporphyrins; D011165:Porphyrinogens; D011166:Porphyrins; D002262:Carboxy-Lyases; D014575:Uroporphyrinogen Decarboxylase