Isolation of the homogeneous constituents from non-diffusible sugar-peptide (NSP) fraction originating from bovine plasma--I. Method of disruption of a sugar-peptide aggregate.
1. High molecular weight sugar-peptide aggregate contains 84--92% of sugar-peptide compounds of the whole non-diffusible sugar-peptide (NSP) fraction obtained from bovine blood plasma by the two-step dialysis method. 2. The aggregate can be resolved into various numbers, dependent on conditions, of electrophoretic homogeneous bands, which appeared to be heterogeneous in thin-layer chromatography on MN-Cellulose. 3. Electrophoresis in 5 M guanidine-hydrochloride allows the disruption of the aggregate and isolation of seven chromatographic and electrophoretic homogeneous constituents with different molecular weights and chemical characters.
D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D004586:Electrophoresis / Q000379:methods; D008970:Molecular Weight; D010455:Peptides / Q000097:blood*
D001786:Blood Glucose; D010455:Peptides