[Purification and isolation of isoenzymes of L-amino acid oxidase from the venom of Bothrops asper].
L-amino acid oxidase (E.C. 1.4.3.2) was purified from the venom of Bothrops asper snakes from Costa Rica. Three forms of this enzyme were obtained from the crude venom using only two steps: heating in the presence of L-leucine and chromatography on DEAE-cellulose. The isozymes were numbered 1, 2a and 2b on the basis of electrophoretic mobility towards the anode. Isozymes 1 and 2b were homogeneous by acrylamide gel electrophoresis and sedimentation velocity. They showed a molecular weight of 125,000 by sedimentation equilibrium.
D000594:Amino Acid Oxidoreductases / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D003435:Crotalid Venoms / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D007527:Isoenzymes / Q000302:isolation & purification*; D050830:L-Amino Acid Oxidase; D007930:Leucine
D003435:Crotalid Venoms; D007527:Isoenzymes; D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase; D007930:Leucine
Purificación y separación de isoenzimas de L-aminoácido oxidasa del veneno de Bothrops asper.