GLC screening and confirmation of barbiturates in post mortem blood specimens.
A quantitative gas chromatographic (GC) method for the screening and confirmation of barbiturates in post mortem blood specimens is presented. Barbiturates were extracted from blood samples and the extracts cleaned-up, concentrated, and then analyzed as free drugs on a 10% UCW-982 GC column. Samples screened positive for free barbiturates were methylated to form 1,3-dimethylbarbiturates, which were then chromatographed on a 3% SE-30 column to confirm the barbiturate identity.
D001463:Barbiturates / Q000097:blood* / Q000506:poisoning; D002849:Chromatography, Gas; D006801:Humans
D001463:Barbiturates