title: The effect of peripheral nerve lesions and of neonatal capsaicin in the rat on primary afferent depolarization.
abstract: 1. In anaesthetized adult rats with intact peripheral nerves, a conditioning orthodromic volley in myelinated afferents produces a prolonged increase in the excitability of the terminals of the afferents which have carried the orthodromic volley and in the terminals of their passive neighbours. This phenomenon, primary afferent depolarization, p.a.d., is shown by the antidromic stimulation of afferents with a micro-electrode in the dorsal horn and by recording the antidromic volley on a cut peripheral nerve.2. If a peripheral nerve is cut and ligated, the size of the myelinated afferent volley generated by stimulation proximal to the cut and measured on dorsal roots is not affected for 14 days after the cut (Wall & Devor, 1981). Similarly the size of the volleys ascending the cord in axons from cord cells remains unaffected.3. If a peripheral nerve is cut and tested after 7-14 days, it produces a markedly decreased p.a.d. on itself and on its neighbours.4. If a nerve is crushed rather than cut, it produces a normal p.a.d. on itself and its neighbours after 7-14 days.5. In animals treated neonatally with capsaicin, there is a severe loss of unmyelinated afferents. Testing the effect of an orthodromic volley in myelinated afferents on the excitability of myelinated afferent central terminals shows that there is a strongly decreased p.a.d. in these animals.6. It is concluded that primary afferent depolarization is sensitive to peripheral nerve lesions and that it may be particularly dependent on the integrity of the unmyelinated afferents in spite of the fact that p.a.d. is measured as a phenomenon of myelinated afferents acting on myelinated afferents.
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002211:Capsaicin / Q000494:pharmacology*; D003714:Denervation; D004558:Electric Stimulation; D005071:Evoked Potentials; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D009411:Nerve Endings / Q000503:physiopathology; D009475:Neurons, Afferent / Q000503:physiopathology*; D059348:Peripheral Nerve Injuries; D051381:Rats; D011919:Rats, Inbred Strains; D013126:Spinal Nerve Roots / Q000503:physiopathology
chemical_list: D005231:Fatty Acids, Unsaturated; D002211:Capsaicin