Modification of liver microsomal lipids by halothane metabolites; a multi nuclear NMR spectroscopic study.
N-trifluoroacetyl phosphatidyl ethanolamine was found as an intermediate of biotransformation of halothane after intense in vivo exposure of non-induced and phenobarbital induced rabbits. The metabolite has been identified in detergent treated liver microsomes by 31P-NMR-spectroscopy and in microsomal lipid extracts by 19F-NMR- and 1H-NMR-spectroscopy as well as by thin layer chromatography. The same exposure changed phospholipid composition of liver microsomal membranes: most prominent was a substantial decrease of the ratio of phosphatidyl ethanolamines to phosphatidylcholines.
D000818:Animals; D001711:Biotransformation; D006221:Halothane / Q000378:metabolism*; D050356:Lipid Metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D011817:Rabbits
D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D006221:Halothane; D010634:Phenobarbital