Gas chromatographic-mass spectrometric determination of aromatic hydrocarbon metabolites from livers of fish exposed to fuel oil.
Metabolites of several two- and three-ring aromatic hydrocarbons (AHs) have been found in livers of English sole exposed to No. 2 fuel oil. Four metabolites of the C2H5-naphthalenes, six of the C3H7-naphthalenes and one each of fluorene, phenanthrene and anthracene have been partially characterized and their concentrations, which ranged from 50 to 1100 ng/g, were determined. Metabolites were separated from the liver matrix using an automated extractor/concentrator. The resulting extract was then purified by high-performance liquid chromatography, and the metabolites were characterized and quantitated by gas chromatography-mass spectrometry.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005399:Fishes; D008401:Gas Chromatography-Mass Spectrometry; D006838:Hydrocarbons / Q000302:isolation & purification*; D008099:Liver / Q000032:analysis*; D010578:Petroleum; D014877:Water Pollution, Chemical
D006838:Hydrocarbons; D010578:Petroleum