Inhibition of electrogenic anion entry into rat liver mitochondria by N,N'-dicyclohexylcarbodiimide.
The carboxyl group reagent dicyclohexylcarbodiimide inhibits the electrogenic entry of Cl- and NO3-into rat liver mitochondria at alkaline pH. The inhibition is time dependent and 50% inhibition is obtained by the addition of 3-4 nmol DCCD/mg protein. The blockage of the pH-dependent anion-conducting pore appears to be unrelated to the other known actions of DCCD on rat liver mitochondria but seems similar to its effect on the uncoupling protein of brown adipose tissue.
D000818:Animals; D002234:Carbodiimides / Q000494:pharmacology*; D002712:Chlorides / Q000378:metabolism*; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology*; D008027:Light; D008930:Mitochondria, Liver / Q000187:drug effects*; D009573:Nitrites / Q000378:metabolism*; D051381:Rats; D012542:Scattering, Radiation; D013997:Time Factors
D002234:Carbodiimides; D002712:Chlorides; D009573:Nitrites; D004024:Dicyclohexylcarbodiimide