Specific 3H-haloperidol binding to dopamine receptors in the anterior byssus retractor muscle of Mytilus edulis.
The anterior byssus retractor muscle (ABRM) of Mytilus edulis has specific dopamine receptors. We carried out a radioligand binding assay for dopamine receptors in ABRM using (3H)-haloperidol as the radioligand. High affinity binding of (3H)-haloperidol has been shown. Scatchard analysis showed a single component of binding with an apparent equilibrium constant (KD) of 1.6 nM and a maximal number of binding sites (Bmax) of 219 fmoles/mg protein. Some dopamine antagonists displaced 3 nM (3H)-haloperidol binding, and the IC50 and Ki-value of these drugs were calculated. Considering these results, this muscle is thought to be suitable for a study of the dopamine receptors.
D000818:Animals; D001667:Binding, Competitive; D049872:Bivalvia / Q000378:metabolism*; D018492:Dopamine Antagonists; D006220:Haloperidol / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism; D011869:Radioligand Assay; D011954:Receptors, Dopamine / Q000378:metabolism*
D018492:Dopamine Antagonists; D011954:Receptors, Dopamine; D006220:Haloperidol