Antibodies to ornithine decarboxylase. Immunochemical cross-reactivity.
L-Ornithine decarboxylase was purified to apparent homogeneity from the kidneys of testosterone-treated mice. Antibodies to ornithine decarboxylase were raised in a rabbit using the purified enzyme. Ouchterlony double diffusion technique revealed a single precipitin line between the antiserum and purified mouse kidney ornithine decarboxylase. The antibodies inhibited ornithine decarboxylase from various tissues of mice and rats to the same extent, indicating a close immunological relationship. S-Adenosyl-L-methionine decarboxylase and L-histidine decarboxylase from mouse kidney as well as ornithine decarboxylase from Escherichia coli were unaffected by the antibodies.
D000818:Animals; D000906:Antibodies; D002262:Carboxy-Lyases / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D007118:Immunoassay; D007668:Kidney / Q000201:enzymology; D008297:Male; D051379:Mice; D009928:Organ Specificity; D009955:Ornithine Decarboxylase / Q000032:analysis / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains
D000906:Antibodies; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase