Studies on a new antibiotic M-92 produced by Micromonospora. II. Isolation and physicochemical properties of M-92 and its components.
A new antibiotic complex, M-92 was isolated from the whole fermentation broth of Micromonospora verruculosa MCRL 0404. The whole broth was mixed with talc and filtered. The filter cake thus obtained was extracted with acidic methanol to give a crude powder of M-92 complex, which was then separated into A (acidic) and N (neutral or weakly acidic) groups. The A group components are soluble in alkaline water (pH 8.5), while the N group components are not. These components were further separated into six major components designated VA-2, BA-4, BA-5, BN-1, BN-2 and BN-3 by silica gel column chromatography. Components with the letter "V" are reddish violet and those with "B" are blue. The IR and UV spectra of these components suggest that their chromophores may be the same or very closely related to one another. The molecular formula of BN-3 was determined to be C29H23NO9 by mass spectrometry. The results of various spectroscopies on BN-3 suggest that M-92 components consisted of chromophores in which juglone (5-hydroxynaphthoquinone) is conjugated with naphthazarin (5,8-dihydroxynaphthoquinone).
D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D002845:Chromatography; D005285:Fermentation; D008847:Micromonospora / Q000032:analysis* / Q000378:metabolism; D009285:Naphthoquinones / Q000302:isolation & purification; D011809:Quinones / Q000032:analysis; D013053:Spectrophotometry
D000900:Anti-Bacterial Agents; D009285:Naphthoquinones; D011809:Quinones; C036968:M 92