Affinity chromatography with specific antibody increases activity and retains antigenicity of ornithine decarboxylase.
An affinity chromatography column with antibody specific to ornithine decarboxylase (ODC) was employed successfully for the retention and subsequent partial elution of ODC with enhanced activity and retention of antigenicity. This may be a useful procedure for further attempts to produce antiserum for identification and characterizations of ODC in vitro and in vivo.
D000818:Animals; D000918:Antibody Specificity; D000941:Antigens; D002262:Carboxy-Lyases / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D004355:Drug Stability; D005260:Female; D007106:Immune Sera; D008099:Liver / Q000201:enzymology; D008297:Male; D009955:Ornithine Decarboxylase / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits / Q000276:immunology; D051381:Rats
D000941:Antigens; D007106:Immune Sera; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase