title: [Isolation of protein of the chymotrypsin inhibitor from honey locust seeds].
abstract: The chymotrypsin inhibitor was isolated from honey locust (Gleditsia triacanthos L.) by chromatography on trypsin- and chymotrypsin-Sepharose 4B and by gel filtration on Sephadex G-75. The inhibitor can inhibit chymotrypsin but has no effect on trypsin, subtilisin and proteinases from Asp. oryzae and Str. griseus. The inhibitor forms a complex with chymotrypsin at a molar ratio of 1:1, has a molecular weight of about 20,000 and a low content of half-cysteine.
mesh_terms: D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D008970:Molecular Weight; D011480:Protease Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D012639:Seeds / Q000032:analysis*
chemical_list: D011480:Protease Inhibitors; D002918:Chymotrypsin
vernacular_title: Vydelenie belka--ingibitora khimotripsina iz semian gledichii.