[NADPH- and ATP-dependent luminescence of extracts from luminous bacteria].
It was shown that the luminescence of extracts prepared from luminous bacteria is stimulated by NADPH and ATP without FMN or long-chain aliphatic aldehydes, which are routinely used for producing luminescence of extracts from luminous bacteria in vitro. In these extracts an aldehyde factor, a natural analog of aliphatic aldehydes, is synthesized. The enzymatic system involved in maintaining the luminescence of NADPH and ATP is probably not coupled with the functioning of NAD(P)H: FMN oxidoreductase which has been supposed to participate in luminescence processes in vivo. It is assumed that both aldehyde factor synthesis and reduction of endogenous analog of FMN, natural substrates of bacterial luciferase, are due to the functioning of the same metabolic pathway.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D005486:Flavin Mononucleotide / Q000494:pharmacology; D007700:Kinetics; D008163:Luminescent Measurements; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009249:NADP / Q000378:metabolism*; D010776:Photobacterium / Q000378:metabolism*
D009249:NADP; D005486:Flavin Mononucleotide; D000255:Adenosine Triphosphate; D009247:NADH, NADPH Oxidoreductases
Zavisimaia ot NADPH i ATP liuminestsentsiia ékstraktov svetiashchikhsia bakteriĭ.