title: Incorporation of 14C stearic acid in lens organ culture.
abstract: 14C stearic acid was incubated in organ culture of rabbit lens for 6, 12 and 20 hours. Stearic acid did penetrate the lens and was incorporated into triacyl-glycerides and phospholipids. It also served as a carbon source for cholesterol synthesis. Individual fatty acids were isolated and radioactivity measurements were made. Incorporation of label was found in C12:0, C14:0 and C16:0 fatty acids, indicating de novo synthesis in the lens; incorporation was found in C16:1 and C18:1, indicating delta 9 desaturase activity, and incorporation was found in the fatty acids of 20 carbons or longer, indicating the presence of a fatty acid elongation system in the lens.
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000096:biosynthesis; D005227:Fatty Acids / Q000032:analysis; D007908:Lens, Crystalline / Q000032:analysis / Q000378:metabolism*; D009924:Organ Culture Techniques; D010743:Phospholipids / Q000096:biosynthesis; D011817:Rabbits; D013229:Stearic Acids / Q000378:metabolism*; D014280:Triglycerides / Q000096:biosynthesis
chemical_list: D005227:Fatty Acids; D010743:Phospholipids; D013229:Stearic Acids; D014280:Triglycerides; D002784:Cholesterol