Direct radioimmunoassay of estradiol-17 beta in defatted bovine milk.
A radioimmunoassay was developed for rapid determination of estradiol-17 beta concentrations in unextracted defatted bovine milk. The assay was dependent on the use of a highly specific anti-estradiol-17 beta antiserum. Application of a formula to correct for the interference associated with individual milk samples and use of appropriate assay blanks facilitated interpolation on a buffer standard curve. The assay offered a high degree of sensitivity (0.6pg/ml milk) and a precision (within-assay coefficient of variation: 0.196; between-assay CV:0.191) comparable with contemporary extraction methods.
D000818:Animals; D002417:Cattle; D004958:Estradiol / Q000032:analysis*; D005260:Female; D008433:Mathematics; D008892:Milk / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods
D004958:Estradiol