The separation of a fraction enriched in purely inhibitory nerve terminals from rat cerebellum.
The separation of a fraction enriched in cerebellar Golgi axon terminals of well-established inhibitory nature is described. The procedure is based on the phenomenon that the incubation of cerebellar glomerulus particles (isolated by a method described earlier) in saline media leads to the selective destruction of mossy terminals and of dendrites synapsing with them, whereas Golgi axon terminals remain intact. A 60-70% enrichment in Golgi terminals was achieved as revealed by electron microscopy.
D000818:Animals; D001369:Axons; D002531:Cerebellum / Q000033:anatomy & histology*; D003712:Dendrites; D009411:Nerve Endings / Q000033:anatomy & histology*; D051381:Rats; D013574:Synaptosomes