[Method of quantitative determination of androgens and their metabolites].
Simple and rapid procedure is developed for quantitative estimation of 3H-androgen metabolites, which is carried out without extraction of labelled steroids from tissue homogenates after incubation with a substrate. After incubation the samples were liophylized and the labelled metabolites were separated using thin-layer chromatography on Silufol plates. The zones, corresponding to labelled metabolites, were scratched out with silica gelsheet and radioactivity was estimated. The procedure excluded practically a loss of labelled steroids in the samples. The procedure was used for estimation of 3H-androgen metabolites in vitro--3H-testosterone, 3H1 dihydrotestosterone and 3H-5 alpha-androsterone-3 beta, 17 beta-diol in homogenates of hypophyses and hypothalamuses from rat males (intact and castrated) and in adenoma of human prostatic gland.
D000728:Androgens / Q000032:analysis / Q000378:metabolism*; D015113:Androstane-3,17-diol / Q000032:analysis*; D000732:Androstanols / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D013196:Dihydrotestosterone / Q000032:analysis*; D006801:Humans; D007031:Hypothalamus / Q000032:analysis; D008297:Male; D008722:Methods; D010902:Pituitary Gland / Q000032:analysis; D011467:Prostate / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000032:analysis*
D000728:Androgens; D000732:Androstanols; D013196:Dihydrotestosterone; D015113:Androstane-3,17-diol; D013739:Testosterone
Metod kolichestvennogo opredeleniia androgenov pri izuchenii ikh metabolizma.