Antitumor effect of human fibroblast interferon on the growth of human melanoma cells implanted in nude mice.
The antitumor effect of human fibroblast interferon (HuIFN-beta) on the growth of human melanoma cells (HMV-1) in vitro and in vivo was examined. In in vitro experiments, HMV-1 cells were highly sensitive to HuIFN-beta compared to other human cells. The cell growth in nude mice was also suppressed by daily administration of HuIFN-beta, depending on the dosage and administration route. The most prominent effect was obtained by intratumoral injection and a lesser therapeutic effect was achieved by subcutaneous injection around the inoculated tumor. Intraperitoneal injection of HuIFN-beta was unsuccessful. Pharmacokinetic studies of HuIFN-beta in nude mouse indicated that the three administration routes gave similar blood plasma levels and decline curves, suggesting that there is no correlation between the plasma HuIFN-beta level and the therapeutic efficacy. However, a relatively high interferon titer was detected for a long time after intratumoral injection of HuIFN-beta. Because of the species specificity of interferon and the use of the nude mouse system lacking T-cell function, the antitumor effect observed in the present study might reflect an anticellular activity of HuIFN-beta retained in the tumor region.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006801:Humans; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D007370:Interferon Type I / Q000008:administration & dosage / Q000097:blood / Q000627:therapeutic use*; D007700:Kinetics; D008545:Melanoma / Q000032:analysis / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000628:therapy; D012878:Skin Neoplasms / Q000032:analysis / Q000473:pathology / Q000628:therapy*; D014183:Transplantation, Heterologous
D007370:Interferon Type I