Magnetically propelled, encapsulated XAD-2 and microphase extraction technique for a rapid quantitation of basic drugs in serum by GC.
A novel, simple, and highly economical technique is described for a rapid gas chromatographic screening and quantitation of basic drugs in serum. The drugs are adsorbed directly form 1 ml of serum onto 400 mg of XAD-2 resin, encapsulated within a rigid, porous, spherical polypropylene capsule, 2 cm in diameter. The capsule also contains a ferromagnetic bar that provides controlled agitation of contents under the influence of a rotating magnet. Resolution and quantitation of samples spiked with 18 basic drugs are provided by a nitrogen detector. The yields are quantitatively linear in both sub-therapeutic and toxic ranges for most drugs; the extracts exhibit minimal background in the range of sensitivities indicated; total extraction time is 20 min.
D001322:Autoanalysis; D002849:Chromatography, Gas / Q000295:instrumentation; D006801:Humans; D008280:Magnetics; D008832:Microchemistry; D004364:Pharmaceutical Preparations / Q000097:blood*; D011137:Polystyrenes
D004364:Pharmaceutical Preparations; D011137:Polystyrenes; C003771:Amberlite XAD-2 resin