The influence of tRNA on interferon induction by poly I:C in Lpa cells.
The kinetics of interferon production, induced by poly I:C in the presence of DEAE dextran, was studied in Lpa cells. Optimal interferon production was obtained at concentration of 10 micrograms/ml poly I:C and 200 micrograms/ml DEAE dextran. Transfer RNA applied to Lpa cells during induction and synthesis of interferon resulted in continuous production of interferon for 24 hrs. The application of tRNA to cells during the shutoff of interferon synthesis had no effect on the kinetics of interferon production.
D000818:Animals; D003637:DEAE-Dextran / Q000494:pharmacology; D007370:Interferon Type I / Q000096:biosynthesis*; D007700:Kinetics; D007739:L Cells / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D011070:Poly I-C / Q000494:pharmacology*; D012343:RNA, Transfer / Q000494:pharmacology*
D007370:Interferon Type I; D012343:RNA, Transfer; D003637:DEAE-Dextran; D011070:Poly I-C