Comparative effects of chemotherapeutic drugs on human and murine hematopoietic progenitors in vitro.
Using semi-solid clonal assay systems, the cytocidal effects of chemotherapeutic drugs on hematopoietic progenitors (CFU-gm, BFU-e, CFU-e) were compared with their effects on K-562 human leukemia cells. The chemotherapeutic drugs used for these experiments were daunorubicin, cytosine arabinoside, vincristine, methotrexate, bis-chlorethyl-nitrosourea and chlorozotocin. After the cells were exposed for either 1 or 24 h to several concentrations of each drug, clear differences in suppressive effects were seen. The experimental applications of this technology and the clinical implication of these results are discussed.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004305:Dose-Response Relationship, Drug; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D007938:Leukemia / Q000188:drug therapy*; D007942:Leukemia, Experimental / Q000188:drug therapy; D051379:Mice
D000970:Antineoplastic Agents