Cytogenetic effects of shale-derived oils in murine bone marrow.
The cytogenetic effects of exposure of mice to shale-derived oils by either skin painting or intraperitoneal injection were examined. Skin painting with 40 mg crude oil every other day for five weeks had no effect on the frequency of chromosomal aberrations observed in the bone marrow. Three daily intraperitoneal injections of 0.5-2.0 ml/kg per day of a crude shale oil from an aboveground retort induced a dose-related increase in the frequency of aberrations observed. A hydrotreated sample of this oil produced a similar pattern of aberration induction, but at lower frequencies. Crude shale oil from a modified in situ retort induced the highest frequency of chromosomal aberrations at the 0.5 ml/kg dose; lower frequencies were induced at the 1.0 ml and 2.0 ml/kg doses. Of the three shale oils tested, only the crude oil from the aboveground retort induced increased frequencies of sister chromatid exchanges and only at doses that also induced structural aberrations. These studies indicate that structural aberration analysis is a more sensitive test than sister chromatid exchange analysis for the type of DNA damage induced by shale-derived oils in murine bone marrow cells.
D000818:Animals; D001853:Bone Marrow / Q000648:ultrastructure*; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D005260:Female; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010578:Petroleum / Q000633:toxicity*; D012854:Sister Chromatid Exchange
D010578:Petroleum