Small-scale isolation and characterisation of human peripheral blood monocytes.
A technique is described for the isolation and characterisation of monocytes from 15 ml of human peripheral blood. After density gradient centrifugation over Ficoll-Hypaque, monocytes are purified further by substrate adherence under carefully defined conditions. The use of a simple microwell slide system permits the production of multiple small-scale monolayer populations which can be characterised further in terms of their histochemical reactivity (combined stain for chloroacetate and non-specific esterases); endocytic capacity (latex particles); Fc (sensitised ox erythrocytes) and C3 (serum-coated yeast) receptor expression; and precursor maturation potential (7-day cultures). Evidence of considerable cellular heterogeneity is presented.
D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003176:Complement C3 / Q000276:immunology; D004705:Endocytosis; D006801:Humans; D009000:Monocytes / Q000502:physiology*; D011951:Receptors, Complement / Q000276:immunology; D011961:Receptors, Fc / Q000276:immunology
D003176:Complement C3; D011951:Receptors, Complement; D011961:Receptors, Fc