A photoinduced cleavage of DNA useful for determining T residues.
Irradiation of 5'-[32P]-phosphate labeled DNA fragments with ultraviolet light in the presence of primary amines followed by piperidine treatment resulted in base-specific cleavage of the DNA chain at T residues, accompanied by a less intensive G reaction. This simple, T greater than G cleavage offers an alternative method for determining T residues in chemical DNA sequencing.
D004247:DNA / Q000528:radiation effects*; D003854:Deoxyribonucleotides / Q000032:analysis; D006147:Guanine / Q000032:analysis; D010761:Phosphorus Radioisotopes; D010782:Photolysis; D010957:Plasmids; D013941:Thymine / Q000032:analysis*; D014466:Ultraviolet Rays
D003854:Deoxyribonucleotides; D010761:Phosphorus Radioisotopes; D006147:Guanine; D004247:DNA; D013941:Thymine