A quantitative cytochemical study of acid phosphatases in hepatocytes of different ploidy classes from aging rats.
A quantitative cytochemical study of isolated hepatocytes from young, middle-aged and old adult rats has shown a doubling of acid naphthol AS-BI phosphatase activity as the hepatocytes shift from the diploid to the tetraploid state, and an overall increase in activity in cells from old animals. There was a complete inhibition of all acid phosphatase activity with 10(-2)M sodium molybdate, but only a 90% inhibition in hepatocytes from livers of rats of 12-25 months-old animals with 10(-2)M sodium fluoride, the resistant activity possibly representing adenyl cyclase activity. In contrast, 10(-2)M ouabain inhibited 70-80% of the activity in hepatocytes from 12-15-months-old animals, this level of inhibition decreasing with increasing age, and implying a decrease in transport ATPase activity and an increased lysosomal phosphatase activity with increasing age of the rats.
D000135:Acid Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000375:Aging; D000818:Animals; D005260:Female; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D010042:Ouabain / Q000494:pharmacology; D011003:Ploidies; D051381:Rats; D011914:Rats, Inbred BN; D012969:Sodium Fluoride / Q000494:pharmacology
D010042:Ouabain; D012969:Sodium Fluoride; D000135:Acid Phosphatase