Synthesis of underpolymerized hyaluronic acid by fibroblasts cultured from rheumatoid and non-rheumatoid synovitis.
Fibroblast cultures were started from synovial tissue of normal controls and patients with seronegative and seropositive rheumatoid arthritis, juvenile rheumatoid arthritis and chronic non-rheumatoid synovitis. Glycosaminoglycan synthesis of the cultures was studied by metabolic labeling with 3H-glucosamine. Molecular weight of hyaluronic acid produced and secreted into culture medium by these fibroblast strains was studied by gel filtration in a Sepharose 2 B column. All fibroblast strains from joints with active inflammation produced hyaluronate with decreased molecular weight. The synthesis of underpolymerized hyaluronate thus appears to be a property of activated synovial fibroblasts common to rheumatoid and non-rheumatoid inflammation.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001171:Arthritis, Juvenile / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D002478:Cells, Cultured; D002675:Child, Preschool; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D013585:Synovitis / Q000378:metabolism*
D006820:Hyaluronic Acid