Serodiagnosis of acute hepatitis B virus infection by a modified competitive binding radioimmunoassay.
Using the Staphylococcus aureus absorption method, we have modified a commercial radioimmunoassay kit for the determination of total antibody to hepatitis B core antigen so that we can now determine the predominant globulin species of that antibody. One-hundred percent of acute hepatitis B sera had a predominance of immunoglobulin M antibody to hepatitis B core antigen, whereas immunoglobulin G antibody to hepatitis B core antigen was predominant in 98.2% of chronic hepatitis B surface antigen carriers. With this test and the commercially available tests for immunoglobulin M anti-hepatitis A virus, one can now reasonably differentiate acute hepatitis B from non A/non B hepatitis in a chronic hepatitis B surface antigen carrier.
D000208:Acute Disease; D000818:Animals; D001667:Binding, Competitive; D002353:Carrier State / Q000175:diagnosis; D006509:Hepatitis B / Q000175:diagnosis*; D006512:Hepatitis B Core Antigens / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods*; D012698:Serologic Tests
D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens