Differential binding affinity of immobilized Concanavalin A-Sepharose 4B for normal and myelomatous immunoglobulins.
Immobilized Concanavalin A-Sepharose 4B (CSB) binds myeloma immunoglobulins (IgG through IgE) in larger proportions than it binds normal immunoglobulins. Some possible explanations for this are discussed. Also, CSB shows differential affinity among immunoglobulins, normal or myelomatous; its greatest affinity is for IgM and IgE. This property can be used to separate a large percentage of the IgM and IgE from both normal and myelomatous sera.
D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D007136:Immunoglobulins / Q000276:immunology*; D007164:Immunosorbents; D009101:Multiple Myeloma / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology*; D011863:Radioimmunoassay; D012685:Sepharose / Q000031:analogs & derivatives / Q000276:immunology*
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D007164:Immunosorbents; D011134:Polysaccharides; C028326:concanavalin A-sepharose; D007073:Immunoglobulin E; D012685:Sepharose