[Sensitivity of the oxygen absorption process in Brevibacterium flavum to pH changes in the medium and the action of an oxidation and phosphorylation uncoupler].
The rate of oxygen uptake (QO2) was shown to increase in the chemostat culture of a Brevibacterium flavum 22 LD auxotrophic strain at D = 0.13 h-1 after addition of chlorocarbonylcyanide phenylhydrazone. The index of respiration control reached 1.6. The QO2 temporarily increased or decreased, respectively, when the quasi steady-state of the culture was disturbed by an acid or alkaline pulse. The quantity of oxygen required for compensating changes in the pH of the medium was expressed as O/H+ and O/OH-ratios which were equal to 3.35 X 10(-2) and 6.25 X 10(-2), respectively. The effect of respiration control was not manifested in the prototrophic B. flavum strain in the conditions of carbon and energy limitation. However, at a carbon substrate excess, the QO2 of the strain increased when the medium was acidified for a short period of time or chlorocarbonylcyanide phenylhydrazone was added to it.
D001951:Brevibacterium / Q000187:drug effects / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006835:Hydrazones / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000378:metabolism*; D010416:Pentachlorophenol / Q000494:pharmacology; D014475:Uncoupling Agents / Q000494:pharmacology*
D003470:Culture Media; D006835:Hydrazones; D014475:Uncoupling Agents; D010416:Pentachlorophenol; D010100:Oxygen
Chuvstvitel'nost' protsessa pogloshcheniia kisloroda u Brevibacterium flavum k izmeneniiam pH sredy i deistviiu razobshchitelia okisleniia i fosforilirovaniia.