Proliferation of human acute leukemia cells in culture on treatment with an homo-aza-steroid.
When cultures of human leukemia cells from 9 untreated patients with acute leukemia were treated with 3 beta-hydroxy-13 alpha-amino-13,17-seco-5 alpha-androstan-17-oic-13, 17-lactam, an increased proliferating activity was exhibited. The leukemia cells were cultured for 48h and the amounts of lactam (4, 12 and 24 ng/ml) were added at zero time in the continuous presence of (3H)-thymidine. Our data suggested that in high concentrations lactam acts as a toxic agent while in the lower ones it seems to stimulate proliferation of the blast cell.
D000328:Adult; D000731:Androstanes; D001378:Azasteroids / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007938:Leukemia / Q000503:physiopathology*; D008875:Middle Aged; D013260:Steroids, Heterocyclic / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
D000731:Androstanes; D001378:Azasteroids; D013260:Steroids, Heterocyclic; C036047:3 beta-hydroxy-13 alpha-amino-13,17-seco-5 alpha-androstan-17-oic-13,17-lactam; D013936:Thymidine