[Effects of high density lipoproteins (HDL) and low density lipoproteins (LDL) on cultured bovine aorta endothelial cells. Preliminary observations on ultrastructural morphology of the plasma membrane by freeze-fracturing].
Bovine aortic endothelial cells (BAEC), detached with collagenase, have been studied morphologically after administration, in vitro, of human lipoprotein fractions (200 micrograms/ml of LDL, 200 micrograms/ml of HDL), 24 h before the confluence. One day after the treated cells and the control one were detached with trypsine and prepared for freeze-etching examination. The plasma-membrane has revealed, in the lipoprotein treated cells (HDL or LDL) an increased average value of the number of "pits" present upon a surface unit (P less than 0,05). Moreover, in the HDL-treated cells, the "pits", sometimes, have appeared with a different morphological evidence, similar to the aspects of exocytosis as seen e.g. in platelets or in Langerhans isle cells.
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology* / Q000187:drug effects; D002417:Cattle; D002462:Cell Membrane / Q000648:ultrastructure; D002478:Cells, Cultured; D004727:Endothelium / Q000648:ultrastructure; D005614:Freeze Fracturing; D008075:Lipoproteins, HDL / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008854:Microscopy, Electron
D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL
Effetti delle lipoproteine ad alta (HDL) e a bassa (LDL) densita' su cellule endoteliali di aorta bovina in coltura. Osservazioni preliminari sulla morfologia ultrastrutturale della membrana plasmatica al crio-decappaggio.