title: [Controlled centrifugation by a w2dt integrator. Application to blood transfusion].
abstract: The centrifugation constitutes a fundamental stage in the blood components preparation as fresh frozen plasma and platelets rich plasma. The measure of the total effect of centrifugation (VT) is achieved with an integrator "w2dt". This parameter characterizes, in an unique way, the preparation procedure of a blood component. The value VT is determined and then sticked on the integrator, which made the rotation stop as soon as the preselected effect of centrifugation is reached. Thanks to this accessory, the authors have analysed the relative importance of the three data: acceleration, constant speed and deceleration, on the total effect of centrifugation. This analysis has induced them to a new approach of the centrifugation. For the authors, the classic notion of rotation time at constant speed, become insufficient. In blood transfusion, several applications are possible. The authors have determined the optimal conditions of platelets rich plasma (yield higher than 80% during a minimum real time) and platelets concentrate preparation with two different centrifuges, Sorvall RC3B and Jouan K 110 SX, equipped with Sorvall Integrator. The reproductibility of the experiments is satisfying when the centrifuged weight is comparatively constant. The standardization of centrifugation technics will be easier when the rotation radius will be included in the calculation of the total effect of centrifugation, thanks to integration.
mesh_terms: D001792:Blood Platelets; D001803:Blood Transfusion; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation / Q000379:methods*; D006801:Humans; D008433:Mathematics; D010976:Platelet Count; D013997:Time Factors
vernacular_title: Centrifugeuses asservies par un intégrateur w2dt. Applications en transfusion sanguine.