[Examination of protein digestibility in individual intestinal segments under in vivo conditions in experimental rats by the isotope dilution method].
Test rats were labelled with 15N over a period of 7 days and killed on the 12th day. 220 min. before they were killed the diet was changed (wheat diet leads to whole egg diet) and they were given a 14C-leucine injection. The 15N-labelling of the digesta proved to be suitable for the determination of the digestibility of the feed proteins in the individual sections of the intestines. If the atom-% 15N-excess of the TCA-soluble fraction of the digesta is = 100%, the resulting digestibility of the protein fraction (TCA-precipitable fraction) corresponds to the 15N-dilution of the unlabelled feed protein. The following digestibility values were ascertained: (formula; see text) The calculation method suggested here cannot be applied to the large intestine because microbial activity influences the quotients. 14C-labelling was also insufficient for gathering statements on the digestibility of proteins.
D000818:Animals; D004044:Dietary Proteins / Q000008:administration & dosage / Q000378:metabolism*; D004063:Digestion; D004527:Egg Proteins / Q000378:metabolism; D007421:Intestine, Small / Q000033:anatomy & histology / Q000502:physiology*; D007930:Leucine / Q000008:administration & dosage; D008297:Male; D010940:Plant Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014908:Triticum
D004044:Dietary Proteins; D004527:Egg Proteins; D010940:Plant Proteins; D007930:Leucine
Prüfung der Proteinverdaulichkeit in einzelnen Darmabschnitten unter in vivo-Bedingungen an Versuchsratten nach der Isotopenverdünnungsmethode.