Use of prolonged treatment and the fluctuation test to detect mutations in human fibroblasts treated with methyl methanesulfonate.
The methyl methanesulfonate (MMS) induction of 6-thioguanine-resistant (6TGr) mutants in non-synchronized human fibroblasts has been quantitatively characterized. A 24-h MMS treatment failed to induce mutations in non-synchronized cultures. However, exposure of growing cells to MMS for 3 days caused a significant increase in the mutation frequencies. The mutation induction was dose-dependent. The fluctuation test was also useful in detecting the mutagenic activity of MMS in human fibroblasts. Prolonged treatment of cultures made the human cell mutagenesis testing system more sensitive.
D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008741:Methyl Methanesulfonate / Q000494:pharmacology*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D010413:Penis; D013866:Thioguanine / Q000494:pharmacology
D009153:Mutagens; D008741:Methyl Methanesulfonate; D013866:Thioguanine