The effect of estrogen on the concentration of ovalbumin gene sequence in the 2 M NaCl residual fraction of oviduct chromatin.
Oviduct chromatin was isolated from both estrogenized and non-estrogenized hens. Extraction of the chromatin with 2 M NaCl removed a majority of the proteins, and the resulting DNA was then separated into two components: (1) a major fraction which was virtually protein-free; and (2) a minor fraction which was complexed with proteins. It was found that the DNA fraction that is complexed with proteins contained ovalbumin gene sequences and that the concentration of these sequences could be boosted by estrogen-treatment.
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000032:analysis*; D004247:DNA / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D005796:Genes; D009693:Nucleic Acid Hybridization; D010047:Ovalbumin / Q000235:genetics*; D010057:Oviducts / Q000032:analysis* / Q000187:drug effects; D012965:Sodium Chloride
D002843:Chromatin; D004967:Estrogens; D012965:Sodium Chloride; D010047:Ovalbumin; D004247:DNA