Euchromatin protein analysis in preneoplastic and neoplastic cell lines: effects of endogenous chromatin solubilization.
No differences were detected in the micrococcal nuclease or deoxyribonuclease I-solubilized (euchromatin) proteins from several preneoplastic and neoplastic mouse epithelial cell lines. Significant levels of endogenous chromatin solubilization were detected in these cell lines prior to the addition of the exogenous nucleases. The endogenous solubilization markedly reduced the susceptibility of the remaining chromatin to solubilization by micrococcal nuclease or deoxyribonuclease I. Evidence is presented which suggest that the endogenous solubilization may obscure differences in the protein components of the euchromatin fraction from these preneoplastic and neoplastic cell lines.
D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000032:analysis*; D003851:Deoxyribonucleases; D004848:Epithelium / Q000032:analysis; D051379:Mice; D008836:Micrococcal Nuclease; D009363:Neoplasm Proteins / Q000032:analysis*; D009374:Neoplasms, Experimental / Q000032:analysis*; D011230:Precancerous Conditions / Q000032:analysis*; D012469:Salivary Glands / Q000032:analysis; D001743:Urinary Bladder / Q000032:analysis
D002843:Chromatin; D009363:Neoplasm Proteins; D003851:Deoxyribonucleases; D008836:Micrococcal Nuclease