Uroporphyrinogen decarboxylase. A method for measuring enzyme activity.
A method for measuring the activity of uroporphyrinogen decarboxylase from a variety of sources is described. Porphyrinogen substrates are generated by enzymic synthesis or chemical reduction. Substrates and enzyme are incubated under standardized conditions. The reactions are then stopped and the reaction products, as well as unmetabolized substrate, are absorbed on tale, eluted and esterified. Porphyrin esters are then separated and quantified using high performance liquid chromatography.
D000818:Animals; D002262:Carboxy-Lyases / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D011163:Hydroxymethylbilane Synthase / Q000378:metabolism; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D011166:Porphyrins / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D014575:Uroporphyrinogen Decarboxylase / Q000032:analysis*
D011166:Porphyrins; D011163:Hydroxymethylbilane Synthase; D002262:Carboxy-Lyases; D014575:Uroporphyrinogen Decarboxylase