title: Rapid, modified oxidase test for oxidase-variable bacterial isolates.
abstract: A modified oxidase reagent, 1% tetramethyl-p-phenylenediamine in dimethyl sulfoxide, proved superior to the routinely used 1% aqueous tetramethyl-p-phenylenediamine dihydrochloride in detecting weakly oxidase-positive gram-negative bacteria after 24 h of growth on agar media (40 of 40 positive versus 22 of 40 positive). The bacterial inoculum was obtained with a cotton-tipped swab instead of a loop or wooden applicator, and the reaction required less than 15 s.
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000201:enzymology; D004121:Dimethyl Sulfoxide; D007202:Indicators and Reagents; D010088:Oxidoreductases / Q000032:analysis*; D013772:Tetramethylphenylenediamine
chemical_list: D007202:Indicators and Reagents; D010088:Oxidoreductases; D013772:Tetramethylphenylenediamine; D004121:Dimethyl Sulfoxide