A comparison of Ca++ incorporation in microsomal fractions from bovine, canine and rabbit aortic smooth muscle.
A comparative study was made of high and low affinity Ca++ uptake (ATP present) and binding (ATP absent) in microsomal fractions prepared by the same method from bovine, canine and rabbit aortic smooth muscle. Rabbit aortic microsomes incorporated slightly more Ca++ under control conditions than did the bovine or canine preparations. The effects of Sr++ (3.0 mM), neomycin sulfate (1.4 mM) and sodium nitroprusside (1.0 mM) on Ca++ incorporation in aortic microsomes from the three species were qualitatively and quantitatively similar. Sr++ inhibits primarily high affinity Ca++ uptake and binding; neomycin inhibits high and low affinity Ca++ binding and nitroprusside inhibits high affinity Ca++ uptake. Addition of oxalate potentiated high affinity Ca++ uptake in aortic microsomes from all three species. Thus, correlations between studies of effects of agents on Ca++ and tension in aortic strips of one of these species and on Ca++ uptake and binding by an aortic microsomal fraction of one of the other two species examined in this study would appear to be based upon qualitatively appropriate similarities.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D004285:Dogs; D066298:In Vitro Techniques; D008861:Microsomes / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D009355:Neomycin / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D010070:Oxalates / Q000494:pharmacology; D019815:Oxalic Acid; D011817:Rabbits; D013045:Species Specificity; D013324:Strontium / Q000494:pharmacology
D010070:Oxalates; D009599:Nitroprusside; D000255:Adenosine Triphosphate; D019815:Oxalic Acid; D009355:Neomycin; D002118:Calcium; D013324:Strontium