Characterization of contractions in mechanically and enzymatically isolated myocardial cells from the rat heart ventricles.
Single spontaneously beating myocardial cells were prepared by mechanical or enzymatical isolation. Using a TV camera and videotape recorder, beating parameters were characterized in different buffer solutions in regard to temperature and integrity of sarcolemma, which was tested by ability of cells to exclude a vital stain, Evans blue. The mechanically disaggregated cells were totally permeable to this stain, but about 60% of the enzymatically isolated myocytes were able to exclude the stain. The myocytes prepared in the presence of calcium were most tolerant to physiological concentration of calcium. Contractions were mainly of phasic type where the velocity of contraction wave was very slow (50-330 microns/s). The velocity was not correlated to the beat rate. Temperature dependence of the velocity was positively correlated with the integrity of sarcolemma. Beat rate was inversely related to the integrity of sarcolemma being slowest in calcium tolerant enzymatically isolated cells. Beat rate of calcium sensitive myocytes was higher but unstable and the temperature dependence of the rate was steeply reduced and even reversed during the incubation. Some calcium tolerant myocytes generated occasionally an electric type of contraction which came in bursts and was characterized by synchronous sarcomere shortening without any contraction wave.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002469:Cell Separation / Q000379:methods*; D003470:Culture Media; D004798:Enzymes / Q000494:pharmacology; D005070:Evans Blue; D006352:Heart Ventricles / Q000166:cytology*; D009200:Myocardial Contraction; D009206:Myocardium / Q000166:cytology*; D051381:Rats; D013696:Temperature
D003470:Culture Media; D004798:Enzymes; D005070:Evans Blue; D002118:Calcium