A modified radio-immune antiglobulin test for the screening of anti-red blood cell antibodies in hybridoma supernatants.
A radio-immune antiglobulin test was developed using mouse monoclonal anti-human A red blood cell (RBC) antibodies, and applied to the screening and study of hybridoma supernatants containing anti-human RBC antibodies. The latter antibodies were provided either as known monoclonal antibodies from culture supernatants and ascitic fluids, or as culture supernatants to be screened. The technical feasibility of the test was greatly facilitated by the use of remova-well disposable polystyrene plates, multichannel pipettes, and multiple-plate centrifuge holders. The sensitivity of the test and its relevance for the screening of hybridoma anti-human RBC antibodies is discussed.
D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology; D001666:Binding Sites, Antibody; D001789:Blood Group Antigens / Q000276:immunology*; D003298:Coombs Test; D004910:Erythrocyte Membrane / Q000032:analysis / Q000378:metabolism; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007518:Isoantibodies / Q000032:analysis*; D051379:Mice
D001789:Blood Group Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007518:Isoantibodies