title: The structure of Artemia sp. haemoglobins-I. Isolation and characterization of oxygen binding domains obtained by limited tryptic digestion.
abstract: Limited tryptic digestion of the extracellular haemoglobins of the crustacea Artemia sp. result in series of discrete fragments which are multiples of 16,000 d. 2. These 16,000 d fragments, together with 50,000 d and 80,000 d fragments have similar amino acid composition and tryptic peptide maps as the intact globin chains. 3. The haem content of the 16,000 d fragments is the same as this of the intact pigment and they can bind oxygen in a non cooperative way. 4. These results strongly support that the 16,000 d fragments represent structural and functional units or domains from which the globin chains are built up.
mesh_terms: D000596:Amino Acids / Q000302:isolation & purification; D000818:Animals; D001156:Artemia / Q000032:analysis*; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D006454:Hemoglobins / Q000302:isolation & purification*; D010101:Oxygen Consumption; D010446:Peptide Fragments / Q000302:isolation & purification*; D014357:Trypsin
chemical_list: D000596:Amino Acids; D006454:Hemoglobins; D010446:Peptide Fragments; D014357:Trypsin