In vitro growth kinetics and thermostability of the golden shiner virus.
In vitro replication of the golden shiner virus (GSV) occurred at 20 C to 30 C in fathead minnow cells; GSV did not replicate at 15 C or 35 C. Optimal replication temperature was approximately 30 C, where the latent period was 8 hr and viral yield was 113 infectious units per cell. Maximum cytopathic effect coincided with maximum released viral titer. Suitable storage temperatures for GSV were -70 C and 4 C. Viral infectivity was rapidly lost at temperatures above 20 C and at normal freezer temperature (-15 C).
D000818:Animals; D002460:Cell Line; D003530:Cyprinidae; D003588:Cytopathogenic Effect, Viral; D004848:Epithelium; D005399:Fishes / Q000382:microbiology*; D012328:RNA Viruses / Q000254:growth & development* / Q000472:pathogenicity; D013696:Temperature; D014774:Virulence; D014779:Virus Replication