All-trans-retinoic acid: measurement of reference values in human serum by high performance liquid chromatography.
A quantitative determination of physiological levels of all-trans-retinoic acid (vitamin A acid) in human serum has been developed. A double-phase extraction of 3.5 ml of serum followed by a specific and sensitive high performance liquid chromatography procedure allowed measurement of levels down to 1 ng/ml. Serum concentrations in 37 fasting volunteers ranged from 2.7 to 4.2 ng/ml and fitted a normal-Gaussian distributional shape with a mean value of 3.5 ng/ml and SD of 0.4 ng/ml, as demonstrated by the Kolmogorov-Smirnov test.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D012016:Reference Values; D013329:Structure-Activity Relationship; D014212:Tretinoin / Q000097:blood*
D014212:Tretinoin