[Physico-chemical properties of the complexes of bacitracin with DNA and its effect on the process of transcription in vitro].
The aim of the present paper was to study the action of one of the peptide antibiotics, bacitracin, as the regulator of gene activity at the transcription level. Therefore the commercial bacitracin has been fractionated into two main parts by paper chromotography. These two fractions have been identified as bacitracin A (biologically active) and bacitracin F (biologically inactive). The binding of both fractions to DNA has been studied. It has been shown that bacitracin A stabilizes DNA to a lesser degree than bacitracin F does. DNA-bacitracin complexes are formed in the major groove of the DNA helix by hydrogen bonds. The analysis of the the obtained experimental data allows us to suppose that bacitracin binding to DNA has a very specific character and that this antibiotic may act as the regulator of gene activity.
D001414:Bacitracin / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology*; D004247:DNA / Q000235:genetics*; D005796:Genes / Q000187:drug effects; D007700:Kinetics; D014158:Transcription, Genetic / Q000187:drug effects*
D001414:Bacitracin; C037039:bacitracin F; D004247:DNA; C026461:bacitracin A
Fiziko-khimicheskie svoĭstva kompleksov batsitratsina s DNK i vliianie ego na protsess transkriptsii in vitro.