[Application of fluorescent probe spectrum analysis for the investigation of the structural state of sorbent. Heterogeneous binding sites].
A method is proposed for analyzing the fluorescence spectra of 1-anilinonaphthalene-8-sulphonate (ANS) and N-phenyl-1-naphthylamine (NPN) in the systems with nonuniform binding regions. Capacities of the method are demonstrated by analysing the fluorescence spectra of probes sorbed on bovine albumin molecules. It has been shown that there are two binding sites of the probes. Relative content of the probes in these binding sites was determined and their structural state was characterized (hydrophoby and microviscosity were estimated). The method described can be used to characterize the structural state of natural and artificial membranes.
D015057:1-Naphthylamine / Q000031:analogs & derivatives; D000817:Anilino Naphthalenesulfonates; D000818:Animals; D002417:Cattle; D005456:Fluorescent Dyes; D011485:Protein Binding; D012710:Serum Albumin, Bovine; D012997:Solvents; D013050:Spectrometry, Fluorescence / Q000379:methods
D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D012997:Solvents; D012710:Serum Albumin, Bovine; C027132:1-anilino-8-naphthalenesulfonate; C005444:N-phenyl-1-naphthylamine; D015057:1-Naphthylamine
Primenenie analiza spektrov fliuorestsentsii zondov dlia issledovaniia strukturnogo sostoianiia sorbenta. Geterogennye uchastki sviazyvaniia.