Measurement of tele-methylhistamine and histamine in human cerebrospinal fluid, urine, and plasma.
A gas chromatographic-mass spectrometric method described by us to measure tele-methylhistamine (t-MH) in brain was used to measure t-MH in human cerebrospinal fluid (CSF), urine and plasma. The presence of t-MH in these body fluids was rigorously established. No pros-methyl-histamine could be detected, and it was used as internal standard to quantify t-MH in the fluids. The mean levels of t-MH were: urine, 943 pmol/mg creatinine; plasma, 12.3 pmol/ml; and CSF, 2.2 pmol/ml. Parallel measurements of histamine by a radioenzymatic method showed, respectively, 182 pmol/mg creatinine; 19.5 pmol/ml; and 388 pmol/ml. The levels of HA in CSF, much higher than those of its metabolite, t-MH, are high enough to stimulate HA receptors in the central nervous system.
D001826:Body Fluids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006632:Histamine / Q000032:analysis* / Q000134:cerebrospinal fluid; D006801:Humans; D008761:Methylhistamines / Q000032:analysis* / Q000134:cerebrospinal fluid
D008761:Methylhistamines; D006632:Histamine; C020404:tele-methylhistamine