Effects of low level of dietary protein and estrogen administration on the aflatoxin-protein complexes in rat liver cytosol.
Effects of protein deficiency and estrogen administration on the aflatoxin-cytosolic protein interactions in rat liver and kidney were examined. In both cases there was significant decrease in aflatoxin B1 ([3H]AFB1) binding to high-Mr proteins. Estrogens, 17 beta-estradiol (EST) and diethylstilbestrol (DES) reduced the binding in two molecular zones containing their receptor proteins. DES administration, however, always increased the amount of total radioactivity bound. Only one-half the liver activity was found in the normal kidney cytosol, and only a fraction of it was complexed to high-Mr proteins. Effects of these modulating agents on cytosolic functions involved in carcinogenesis have been discussed.
D000348:Aflatoxins / Q000378:metabolism*; D000818:Animals; D003600:Cytosol / Q000378:metabolism*; D004044:Dietary Proteins / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D011916:Rats, Inbred F344
D000348:Aflatoxins; D004044:Dietary Proteins; D004967:Estrogens