title: Endothelial specific granules in the umbilical veins of the postnatal rabbit.
abstract: A remarkable increase in number of endothelial specific granules was observed in the rabbit umbilical veins between 2 and 5 days after birth. Electron microscopy indicated that the granules were segregated in the Golgi complex of the endothelial cells and released into the vascular lumen during the postnatal obliteration stage of this vessel. Incubation of the postnatal vessels in Ringer solution containing a histamine-releasing compound induced remarkable morphological alterations of these cytoplasmic components; a reduction of their osmiophilia, swelling with a widened space separating the granular matrix from the limiting membrane, fusion to each other and expulsion of their contents into the vascular lumen, as in mast cell degranulation by this drug, were noted. High-performance liquid chromatography of the homogenized vessels demonstrated appreciable concentrations of histamine in the postnatal samples. There was a correlation between the histamine concentration and the quantity of granules in the respective postnatal samples. The present study strongly suggests that the granules are reservoirs of histamine and have an important role in the obliteration of this vessel.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002462:Cell Membrane / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000032:analysis / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D004727:Endothelium / Q000648:ultrastructure*; D005089:Exocytosis; D006056:Golgi Apparatus / Q000648:ultrastructure; D006632:Histamine / Q000032:analysis; D011817:Rabbits; D014471:Umbilical Veins / Q000648:ultrastructure*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine