Effects of ouabain and valinomycin on in vitro erythroid colony formation (CFU-e and BFU-e).
Ouabain enhances the number of clonally derived erythroid stem cell colonies (CFU-e and BFU-e) from normal murine bone marrow. Ouabain is known to inhibit lymphocyte proliferation by blocking the Na+/K+ pump. To further explore these Na+/K+ ATPase-associated interrelationships, valinomycin, an inhibitor of Na+/K+ ATPase was employed. The addition of valinomycin (10(-11) to 10(-15) M) in the presence of ouabain and erythropoietin (Ep) did not alter the erythroid colony-forming stimulation which was characteristic of cultures in which only ouabain (10(-15) M) and Ep were added. Valinomycin did effectively block both CFU-e and BFU-e formation when it was added in the range of 10(-3) to 10(-9) M. The mechanism of this valinomycin-induced inhibition and ouabain stimulation is discussed.
D000818:Animals; D001853:Bone Marrow / Q000502:physiology; D002478:Cells, Cultured; D002999:Clone Cells; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010042:Ouabain / Q000494:pharmacology*; D014634:Valinomycin / Q000494:pharmacology*
D014634:Valinomycin; D010042:Ouabain