Inhibition of interferon secretion in human fibroblast by vinblastine.
Vinblastine treatment of superinduced human fibroblast (FS-4) cultures strongly inhibited interferon (IFN) secretion and resulted in intracellular accumulation of IFN activity. The cell-associated IFN migrated slower than secreted IFN in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, indicating a larger size. The results suggest the presence of a possible precursor form of mature HuIFN-beta with biological activity.
D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism*; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000378:metabolism; D014747:Vinblastine / Q000494:pharmacology*
D007370:Interferon Type I; D011498:Protein Precursors; D014747:Vinblastine