A rapid, quantitative GLC method for the simultaneous determination of nicotine and cotinine.
Published gas-liquid chromatographic (GLC) methods for the determination of nicotine and cotinine have proved impractical for the analysis of a large number of clinical samples. Significant improvements over other methods have been achieved, being low sample volume (0.5 mL plasma), rapid two-step extraction from plasma, no evaporation step, and good separation. The lower limits of sensitivity for nicotine and cotinine were 1 and 5 ng/mL, respectively. The method was validated by the analysis of plasma samples from cigarette-smoking volunteers. The method described permits the quick, routine determination of nicotine and cotinine in a large number of samples.
D002849:Chromatography, Gas; D003367:Cotinine / Q000097:blood*; D006801:Humans; D009538:Nicotine / Q000097:blood*; D011760:Pyrrolidinones / Q000097:blood*; D012907:Smoking
D011760:Pyrrolidinones; D009538:Nicotine; D003367:Cotinine