Versatile method for evaluating a cell culture by various morphological techniques.
Cellulose acetate is a versatile material for evaluating cells grown under identical conditions by various morphological techniques. This inexpensive material is transparent, easily cut to size and shape, nontoxic to cell cultures, and resistant to most chemicals used in histochemistry and in scanning and transmission electron microscopy. Samples may be obtained during and after the culture process. Cellulose acetate slides can be mounted directly over glass slides for direct observation and are easily peeled off plastic blocks for electron microscopy, leaving the cells behind. Relative disadvantages include its autofluorescence and a tendency to soften in strong acids or pure solutions of organic solvents such as xylene and propylene oxide.
D002478:Cells, Cultured; D002482:Cellulose / Q000031:analogs & derivatives*; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D012160:Retina / Q000166:cytology
C005062:acetylcellulose; D002482:Cellulose