Involvement of calmodulin in granulocyte chemotaxis: the effect of calmodulin inhibitors.
Chemotaxis and locomotion in rabbit peritoneal granulocytes are strongly inhibited by trifluoperazine and N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), both known as calmodulin inhibitors. This inhibition occurs in a concentration range where cell damage is not significant. Although inhibition was most pronounced in experiments with Ca2+ and Mg2+ present in the medium, inhibition of chemotaxis by trifluoperazine and W-7 was also observed in the absence of these ions. The results suggest that calmodulin is involved in granulocyte chemotaxis and locomotion.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000502:physiology*; D002147:Calmodulin / Q000037:antagonists & inhibitors / Q000502:physiology*; D002465:Cell Movement / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D006098:Granulocytes / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D009239:N-Formylmethionine / Q000031:analogs & derivatives / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009842:Oligopeptides / Q000494:pharmacology; D011817:Rabbits; D013449:Sulfonamides / Q000494:pharmacology; D014268:Trifluoperazine / Q000494:pharmacology
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D009842:Oligopeptides; D013449:Sulfonamides; D014268:Trifluoperazine; D009239:N-Formylmethionine; D009240:N-Formylmethionine Leucyl-Phenylalanine; C017967:W 7; D008274:Magnesium; D002118:Calcium