The influence of inhibitors of cellular synthesis and UV irradiation on interferon induction by RNA from Piptoporus betulinus.
The effect of cycloheximide, chloramphenicol, ethidium bromide (EB), aurin tricarboxylic acid (ATA), and actinomycin D on the production of interferon (IFN) in human embryo fibroblasts (HAT) and L929 cells, stimulated with RNA from Piptoporus betulinus (Pb-RNA) was studied. Treatment of HAT cells with cycloheximide superinduced the production of IFN. Subsequent treatment with cycloheximide and actinomycin D chloramphenicol and actinomycin D or ATA and actinomycin D early after induction also enhanced the production of interferon, but the highest titers of interferon were observed when HAT cells were superinduced with cycloheximide and actinomycin D 11 hours after induction. In L929 cells the superinduction was efficient shortly after induction, but the IFN titers obtained were still very low. The effect of UV irradiation of HAT cells was also examined, but no enhancement of IFN production after induction with Pb-RNA was observed. Pb-RNA produced IFN in the spleen and liver of Swiss mice but no enhancement of IFN production was observed after treatment of mice with cycloheximide, ATA and EB.
D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology*; D002478:Cells, Cultured; D004396:Coloring Agents / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology; D007370:Interferon Type I / Q000096:biosynthesis*; D007739:L Cells / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D012331:RNA, Fungal / Q000494:pharmacology*; D014466:Ultraviolet Rays
D000963:Antimetabolites; D004396:Coloring Agents; D007369:Interferon Inducers; D007370:Interferon Type I; D012331:RNA, Fungal