Kinetic analysis of H+/methyl beta-D-thiogalactoside symport in Saccharomyces fragilis.
A theoretical description of initial uptake kinetics of H+/sugar symport is given, with emphasis on the differences between carrier and non-carrier systems. Transport of methyl beta-D-thiogalactoside in Saccharomyces fragilis is shown to proceed via the inducible lactose transporter. Uptake of this sugar stimulates electrogenic H+ influx. Together with the correlation between methyl beta-D-thiogalactoside accumulation and the proton-motive force this shows that transport proceeds via H+ symport. Kinetic analysis of initial influx revealed that transport proceeds via a single transport system, sensitive to changes in membrane potential. The pH dependence of the kinetic parameters showed that Kapp is almost pH insensitive, whereas Vapp decreases strongly at increasing extracellular pH. It is shown that transport proceeds, most likely, via a non-carrier system, with random binding of H+ and sugar, in a system where binding of the first ligand does not influence binding of the second.
D001692:Biological Transport; D002462:Cell Membrane / Q000502:physiology; D029968:Escherichia coli Proteins; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008433:Mathematics; D008564:Membrane Potentials; D026901:Membrane Transport Proteins / Q000378:metabolism*; D008756:Methylgalactosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins; D012440:Saccharomyces / Q000378:metabolism*; D027981:Symporters; D013862:Thiogalactosides / Q000378:metabolism*; D013865:Thioglycosides / Q000378:metabolism*
D029968:Escherichia coli Proteins; C050221:LacY protein, E coli; D026901:Membrane Transport Proteins; D008756:Methylgalactosides; D008759:Methylglycosides; D009004:Monosaccharide Transport Proteins; D027981:Symporters; D013862:Thiogalactosides; D013865:Thioglycosides; C009468:thiomethylgalactoside; C021499:lactose permease