Simplification of the procedure for determining erythrocyte glyceraldehyde 3-phosphate dehydrogenase activity.
The in vitro assay procedure for erythrocyte glyceraldehyde 3-phosphate dehydrogenase (G3PD) entails the suspension of cells in a hypotonic hemolyzing solution followed by a freeze-thaw step, presumably to elute the enzyme from the membrane phase. Data outlined in this report indicate that the freezing and thawing of red cells suspended in hypotonic solution does not cause significant elution of this enzyme from the membrane and that the accessibility of the enzyme for the in vitro procedure for measurement can be simplified by omitting the freeze-thaw step.
D004912:Erythrocytes / Q000201:enzymology*; D005615:Freezing; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000097:blood*; D006801:Humans; D013048:Specimen Handling
D005987:Glyceraldehyde-3-Phosphate Dehydrogenases