Isolation of glucagon-37 (bioactive enteroglucagon/oxyntomodulin) from porcine jejuno-ileum. Isolation of the peptide.
A 37 amino acid-peptide has been isolated from porcine jejuno-ileum on the basis of its glucagon-like activity in liver (interaction with glucagon-binding sites and activation of adenylate cyclase) using gel filtration, ion-exchange and high-performance liquid chromatography. Depending on the criteria chosen, this peptide is referred to as either 'bioactive enteroglucagon' (activity in liver), 'oxyntomodulin' (specific action in gastric oxyntic glands) or 'glucagon-37' (chemical structure).
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D005768:Gastrointestinal Hormones / Q000302:isolation & purification*; D004763:Glucagon-Like Peptides / Q000302:isolation & purification*; D007082:Ileum / Q000032:analysis; D066298:In Vitro Techniques; D007583:Jejunum / Q000032:analysis; D008099:Liver / Q000378:metabolism; D009130:Muscle, Smooth / Q000032:analysis*; D053772:Oxyntomodulin; D051381:Rats; D013552:Swine
D005768:Gastrointestinal Hormones; D053772:Oxyntomodulin; D004763:Glucagon-Like Peptides; D000262:Adenylyl Cyclases