Effect of histamine, H1 and H2 agonists on E-rosette formation in normal and atopic subjects.
The effect of histamine on the capacity of human peripheral blood lymphocytes to form E rosettes was studied in normal and atopic subjects. Histamine had little effect on E-rosette formation (E-RF) in atopic subjects, but significantly inhibited E-RF in normal subjects in a dose-dependent fashion. To investigate this phenomenon further, specific histamine type 1 and 2 receptor agonists were used in the rosette assay. The H1 receptor agonist caused significant inhibition of E-RF in both normal subjects and atopics, whereas the H2 agonist produced significant inhibition of E-RF in normal but not atopic subjects. The results indicate a histamine type 2 receptor dysfunction in a subpopulation of atopic peripheral blood T lymphocytes.
D000818:Animals; D001667:Binding, Competitive; D004306:Dose-Response Relationship, Immunologic; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D008761:Methylhistamines / Q000494:pharmacology; D009439:Neuraminidase / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D011969:Receptors, Histamine H1 / Q000187:drug effects; D011970:Receptors, Histamine H2 / Q000187:drug effects; D012397:Rosette Formation; D012756:Sheep
D008761:Methylhistamines; D011725:Pyridines; D011969:Receptors, Histamine H1; D011970:Receptors, Histamine H2; C025566:4-methylhistamine; D006632:Histamine; C006183:2-(2-aminoethyl)pyridine; D009439:Neuraminidase