Association of cap binding protein-related polypeptides with cytoplasmic RNP particles of chick embryonic muscle.
Cap binding protein (CBP)-related polypeptides were identified in different cytoplasmic RNP particles of embryonic chick muscles using monoclonal antibody to purified CBP. A single immunoreactive peptide (Mr 78 000) was present in preparations of both free mRNP particles and a novel 10 S translation inhibitory RNP particle. In contrast, proteins isolated from these particles showed two new low-Mr immunoreactive peptides (Mr 43 000 and Mr 29 000). No CBP related protein could be detected in polysomal mRNP, although an immunoreactive Mr 43 000 CBP-related protein was present in polysomes. The relevance of the association of different CBP-related polypeptides with cytoplasmic RNP particles and polysomes are discussed.
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002642:Chick Embryo; D003432:Cross-Linking Reagents / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009132:Muscles / Q000378:metabolism*; D009698:Nucleoproteins / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism; D039381:RNA Cap-Binding Proteins; D012333:RNA, Messenger / Q000378:metabolism; D012261:Ribonucleoproteins / Q000302:isolation & purification / Q000378:metabolism*
D002352:Carrier Proteins; D003432:Cross-Linking Reagents; D009698:Nucleoproteins; D039381:RNA Cap-Binding Proteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins