title: Nerve growth factor: subunit interactions in the mouse submaxillary gland 7S complex.
abstract: Sedimentation velocity analyses of the isolated subunits of mouse 7S nerve growth factor (NGF) and their binary complexes have been performed. The 2.5S (beta) form of NGF binds 2 alpha subunits independent of the presence of gamma subunits. Two gamma subunits are also complexed by 2.5S NGF; however, this interaction is dependent on the presence of a carboxyl terminal arginine residue on the beta polypeptide chain. The partial loss of this residue in 2.5S NGF preparations results in a ternary complex(es) with a reduced sedimentation coefficient(s). The two binary complexes formed (alpha 2-beta and beta-gamma 2) show markedly different pH stability profiles. The alpha and gamma subunits do not form a demonstrable complex, but apparently can interact in the ternary complex to render it more stable than the sum of the alpha-beta and beta-gamma interactions.
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009414:Nerve Growth Factors; D010455:Peptides / Q000032:analysis; D013363:Submandibular Gland / Q000032:analysis*; D014461:Ultracentrifugation
chemical_list: D009414:Nerve Growth Factors; D010455:Peptides