Modification and evaluation of the potato disc assay and antitumor screening of euphorbiaceae seeds.
Galsky et al. (10, 11) have reported that the inhibition and growth of crown gall tumors, initiated on potato discs by Agrobacterium tumefaciens, apparently has good agreement with 3PS in vivo antileukemic activity in mice. We have now modified and evaluated this assay for its potential use as a prescreen and fractionation monitor of plant extracts for 3PS activity. The modified assay was performed on a series of natural compounds and plant extracts (known to have 3PS activity) and on extracts of seeds of 41 Euphorbiaceae species. The results suggest that the potato disc assay is a safe, simple, rapid, in-house, low cost, prescreen for 3PS antitumor activity; it detects some false positives, but few false negatives; it is statistically much more predictive (p = 0.002) of 3PS activity than either the 9KB (p = 0.140) or the 9PS (p = 0.114) cytotoxicity assays. Its extended use could easily obviate the expense and extensive need for animals in the initial stages of antitumor screening and plant fractionation.
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic; D004121:Dimethyl Sulfoxide; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006801:Humans; D007942:Leukemia, Experimental / Q000188:drug therapy; D051379:Mice; D009374:Neoplasms, Experimental / Q000188:drug therapy; D010936:Plant Extracts / Q000494:pharmacology; D010941:Plant Tumors; D012639:Seeds
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D004121:Dimethyl Sulfoxide