Effect of plasma interference on different vasopressin antisera.
The effect of plasma interference upon 2 AVP antisera both of high affinity and specificity, was investigated. Chromatography of plasma from a normal subject on Sephadex G-25 gave 3 peaks of AVP-like material with antibody A eluting in the void volume, salt volume and in the AVP volume; the quantity of AVP-like material detected in the void volume was 95% less with antibody B. Similar elution profiles were observed in a patient with cranial diabetes insipidus but the third peak was absent. AVP-like material in the first 2 peaks was probably due to interference in the radioimmunoassay. Extraction of plasma by Florisil reduced the first 2 peaks (antibody A) and eliminated the void volume peak (antibody B). Plasma interference affected the absolute values of plasma AVP concentrations of osmotically-stimulated normal and diabetic subjects. AVP antisera displaying least plasma interference are recommended for AVP radioimmunoassay.
D000918:Antibody Specificity; D001127:Arginine Vasopressin / Q000097:blood* / Q000276:immunology; D002850:Chromatography, Gel / Q000379:methods; D006801:Humans; D007106:Immune Sera; D011863:Radioimmunoassay
D007106:Immune Sera; D001127:Arginine Vasopressin