Alterations of NADPH-generating and drug-metabolizing enzymes by feed restriction in male rats.
Two groups of weanling male Sprague-Dawley rats were fed a semisynthetic diet (ad libitum group) and 50% of that consumed by the ad libitum group (restricted group) for 49 days. Compared to the ad libitum group, the animals of the restricted group gained 55% less in body weight and had 60% smaller livers. While serum glucose, urea nitrogen, uric acid and lipids were lowered in the restricted animals, other serum biochemical parameters remained unchanged. The in vitro activities of hepatic drug-metabolizing enzymes were increased two- to threefold in the restricted animals. Feed restriction also caused a significant increase in the activities of NADPH-generating enzymes of liver and adipose tissue; however, the enzymes not concerned with the production of NADPH remained unaffected by feed restriction. It is concluded that feed restriction, unlike starvation, enhanced activities of drug-metabolizing enzymes as well as NADPH-generating enzymes.
D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D005227:Fatty Acids / Q000378:metabolism; D005508:Food Deprivation / Q000502:physiology*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000201:enzymology*; D008297:Male; D009249:NADP / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D000637:Transaminases / Q000378:metabolism
D005227:Fatty Acids; D008055:Lipids; D004364:Pharmaceutical Preparations; D009249:NADP; D010088:Oxidoreductases; D000637:Transaminases