Micro-bore high-performance liquid chromatography for the analysis of pharmaceutical compounds.
A micro-bore high-performance liquid chromatographic (HPLC) system has been constructed using commercially available instruments and pre-packed micro-bore columns. The flow-rates to obtain the highest efficiency for a reversed-phase column and for a silica column are ca. 20 microliters/min and 5 microliters/min, respectively. The micro-bore HPLC system is sixteen times more sensitive than a conventional HPLC system for detection of a compound. With little or no modification in the composition of mobile phases used in conventional HPLC, micro-bore HPLC attains high column efficiency and peak resolution for the analysis of antibiotics, steroids, ibuprofen and sulfonylurea. Micro-bore HPLC has been demonstrated to be capable of performing high speed analysis with a relative standard deviation of ca. 1%.
D000900:Anti-Bacterial Agents / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007052:Ibuprofen / Q000032:analysis; D008832:Microchemistry; D004364:Pharmaceutical Preparations / Q000032:analysis*; D013256:Steroids / Q000032:analysis; D013453:Sulfonylurea Compounds / Q000032:analysis
D000900:Anti-Bacterial Agents; D004364:Pharmaceutical Preparations; D013256:Steroids; D013453:Sulfonylurea Compounds; D007052:Ibuprofen