Selective excretion of IgA in rat bronchial secretions: lack of significant contribution from plasma IgA.
Concentrated rat bronchial washings (BW) were analyzed by gel-filtration and immunochemical methods. BW contained mainly albumin, transferrin and IgG. Free secretory component and secretory IgA were identified in BW; the BW-IgA had the same three sedimentation coefficients, i.e. +/- 11 S, 13 S, 15 S by sucrose density gradient ultracentrifugation, as rat milk and rat bile IgA; the three peaks were secretory IgA. Compared to serum, and relatively to albumin, BW were significantly enriched in IgA, although much less than rat bile. Purified polyclonal rat polymeric 125I-IgA was injected intravenously into normal rats, and into rats with bile duct ligation or partial hepatectomy, which decrease the liver plasma-to-bile transfer of IgA. BW were then collected, one or four hours later, to assess the recovery of the 125I-IgA in BW and to estimate the contribution of serum IgA to BW-IgA. Very little 125I-IgA (less than 0.2%) was recovered in all BW. The specific activity, measured only in the rat with the highest recovery in BW, was 20 times lower in BW than in serum. The data demonstrate that rat serum IgA does not contribute significantly to IgA in BW.
D000418:Albumins / Q000032:analysis; D000818:Animals; D001646:Bile / Q000032:analysis / Q000276:immunology; D001980:Bronchi / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D004587:Electrophoresis, Agar Gel; D005260:Female; D007070:Immunoglobulin A / Q000032:analysis* / Q000378:metabolism; D007074:Immunoglobulin G / Q000032:analysis; D007457:Iodine Radioisotopes; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D007507:Therapeutic Irrigation; D014168:Transferrin / Q000032:analysis
D000418:Albumins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D014168:Transferrin