Isolation, properties and P content of the human brain myosin.
KCl-, and NaCl-myosins were prepared from different parts of the central nervous system (CNS). Throughout these experiments P and lipid contents were higher in NaCl-myosins than in KCl-preparations. Both KCl-, and NaCl-myosins have increased lipid and P contents compared with skeletal muscle myosins. When the specimens were separated by a molecular sieve, it was found by chromatographic technique on Sepharose 4B column that the cerebral and cerebellar myosins were composed of two fractions of different molecular mass while the brain stem and spinal cord myosins revealed only a single peak. The myosin fractions' Ca-ATPase activity could be augmented by rabbit muscle actin. The myosin preparations developed filamentous systems and aggregates which could be shown by scanning electron microscopy. All the CNS-myosin preparations could be phosphorylated; however, they were saturated to a different degree and were influenced by the presence or absence of serotonin. The kinetic studies revealed that the phosphate saturation of the brain stem, cerebellar and cerebral myosins depended on the ATP concentration and incubation time. The alkaline hydrolysates of lipid-free human brain myosin preparations contained amino acid phosphates, P-Arg, P-Lys and P-His in different amounts depending on their sources. In response to a phosphorylating mixture only the amount of P-Arg was elevated in the cerebral myosins, P-Arg and P-His in the brain stem preparations, and P-Arg, P-His and the amounts of unidentified compounds in the cerebellar ones.
D000328:Adult; D000368:Aged; D001923:Brain Chemistry; D001933:Brain Stem / Q000032:analysis; D002531:Cerebellum / Q000032:analysis / Q000648:ultrastructure; D002540:Cerebral Cortex / Q000032:analysis / Q000648:ultrastructure; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D008875:Middle Aged; D009218:Myosins / Q000032:analysis / Q000302:isolation & purification*; D010758:Phosphorus / Q000032:analysis*; D010766:Phosphorylation; D013116:Spinal Cord / Q000032:analysis
D010758:Phosphorus; D009218:Myosins