[The macrophage electrophoresis mobility (MEM) test for detection of cell sensitivity: technical analyses. I. Guinea pig peritoneal cells as indicator cells in the MEM test].
The influence of cell selection and the time of harvesting the indicator cells (peritoneal cells of the guinea pig) on the test result was studied. In order to obtain positive test results, it is not necessary to select the cells. The time of preparation of peritoneal cells following paraffin oil application has no effect on the test result. Peritoneal cells from animals not stimulated with oil also yielded positive MEM test results. 'False negative' results in MEM studies are, to some extent, attributable to the macrophage indicator cell system.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D002452:Cell Count; D002464:Cell Migration Inhibition; D002469:Cell Separation / Q000379:methods*; D006168:Guinea Pigs; D008222:Lymphokines / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D010232:Paraffin / Q000494:pharmacology
D008222:Lymphokines; D010232:Paraffin
Der Makrophagen-Elektrophorese-Mobilitäts-(MEM-) Test zum Nachweis zellulärer Sensibilisierungen: Methodische Untersuchungen. I. Peritonealzellen des Meerschweinchens als Indikatorzellen im MEM-Test.