title: Solution conformation of the biantennary N-linked oligosaccharide of human serotransferrin using 1H NMR nuclear Overhauser effect measurements.
abstract: The conformation in solution of the biantennary complex type oligosaccharide unit derived from human serotransferrin has been investigated using 1H-1H Nuclear Overhauser Effect (NOE) measurements at 300 MHz. From quantitation of the NOE, the alpha(1-3) antenna is shown to exist in a preferred solution conformation with respect to the mannosyl-chitobiose core. The flexibility of the alpha(1-6) arm, together with the absence of NOE data between this arm and the core, indicates that, in contrast to the alpha(1-3) arm the alpha(1-6) arm has no preferred conformation with respect to the core.
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006801:Humans; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D009844:Oligosaccharides; D014168:Transferrin
chemical_list: D009844:Oligosaccharides; D014168:Transferrin