Cytologic detection of virus-specific DNA by in situ hybridization in Syrian hamster embryo cells transformed by viral or combined chemical and viral treatment.
A series of simian adenovirus 7 (SA7)-transformed Syrian hamster cell lines that had T antigen and produced tumors in neonatal hamsters were examined by radioautography in conjunction with in situ molecular hybridization to determine the extent to which the viral genome is integrated on specific chromosomes and the consequences of such integration on sister chromatid exchange (SCE) frequency. No consistent cytological or chromosomal localization of viral DNA in situ hybridization with viral complementary [3H]RNA was detected, even though all lines had an average of one or two viral genomes per cell by molecular hybridization. The SA7 DNA appears to be inserted randomly among the chromosomes and nuclei.
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D006224:Cricetinae; D004279:DNA, Viral / Q000378:metabolism*; D005814:Genes, Viral; D007621:Karyotyping; D008647:Mesocricetus; D009693:Nucleic Acid Hybridization; D012854:Sister Chromatid Exchange
D004279:DNA, Viral