The interaction of DMSO-induced murine Friend leukaemia erythroblasts with cultured syngeneic mouse peritoneal macrophages: an in vitro model for the study of ineffective erythropoiesis.
The interaction between dimethyl sulphoxide-induced Friend leukaemia erythroblasts (IFLE) and cultured syngeneic mouse peritoneal macrophages (PM phi) was assessed radiochemically as well as by light and electron microscopy. The data revealed that a small number of the IFLE either became attached to or were ingested by the PM phi and that the interacting IFLE were usually well differentiated. Interactions of a similar nature were also seen when IFLE were co-cultured with macrophages derived from mouse bone marrow. The extent of interaction between IFLE and PM phi provides a useful system in which mechanisms underlying ineffective erythropoiesis can be defined.
D000818:Animals; D001854:Bone Marrow Cells; D002450:Cell Communication; D002452:Cell Count; D002454:Cell Differentiation; D002478:Cells, Cultured; D006224:Cricetinae; D004121:Dimethyl Sulfoxide; D004900:Erythroblasts / Q000502:physiology*; D004912:Erythrocytes / Q000502:physiology*; D004920:Erythropoiesis; D005260:Female; D007942:Leukemia, Experimental / Q000139:chemically induced / Q000503:physiopathology*; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008811:Mice, Inbred DBA; D008854:Microscopy, Electron
D004121:Dimethyl Sulfoxide