Backcross test demonstrates the linkage of glucocorticoid-induced cleft palate susceptibility to H-2.
Data in this report demonstrate a linkage between glucocorticoid-induced cleft palate susceptibility and H-2. B10.A X C57BL/10F1 females were backcrossed to C57BL/10 males. Cleft palate was induced in the BC1 fetuses by giving pregnant females 80 mg/kg of dexamethasone intraperitoneally (IP) on day 12 of gestation. The H-2a/b haplotype was detected on day 18 BC1 fetal spleen cells with an indirect immunofluorescent labeling technique. Fetuses without antigen H-2Kk were H-2b/b . H-2a/b fetuses had a cleft palate frequency of 29%; 11% of the H-2b/b fetuses had cleft palate (P less than .01), demonstrating that the higher susceptibility to cleft palate segregates with the H-2a halotype.
D000818:Animals; D002972:Cleft Palate / Q000139:chemically induced* / Q000276:immunology; D004198:Disease Susceptibility; D008040:Genetic Linkage; D005938:Glucocorticoids / Q000633:toxicity*; D006183:H-2 Antigens / Q000032:analysis*; D051379:Mice; D008815:Mice, Inbred Strains
D005938:Glucocorticoids; D006183:H-2 Antigens