Sensitization of normal mouse lymphocytes immunized in vitro as detected by macrophage electrophoretic mobility (MEM) test.
Lymph node cells from normal mouse donors, sensitized in vitro with syngeneic chemically induced sarcoma cells, release a macrophage slowing factor after interaction with solubilized tumour-associated antigen. The reaction is specific, i.e., MSF can be detected in the MEM test, if TAA for challenge are prepared from the tumour used for sensitization.
D000818:Animals; D004586:Electrophoresis / Q000379:methods; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D012513:Sarcoma, Experimental / Q000276:immunology*