Requirement for a reduced sulphydryl entity in the protection of molybdate-stabilized estrogen receptor.
The separate and combined effect of molybdate and dithiothreitol on the stability of human uterine 9 S estrogen receptor were studied. Maximal, short-term, protection of the 9 S estrogen receptor was achieved by the joint inclusion of both stabilizing agents in cytosol buffers. This molybdate--dithiothreitol-mediated stability was dependent on reducing agent concentration inferring sulphydryl involvement in 9 S receptor protection by molybdate. The study also showed that molybdate--dithiothreitol could not prevent the gradual decay of the 9 S estrogen receptor to the 4 S form in cytosols stored at 4 degrees C over prolonged periods.
D002021:Buffers; D003600:Cytosol / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006801:Humans; D007700:Kinetics; D008982:Molybdenum / Q000494:pharmacology*; D009215:Myometrium / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000187:drug effects / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism*
D002021:Buffers; D011960:Receptors, Estrogen; D004958:Estradiol; D008982:Molybdenum; C024687:sodium molybdate(VI); D004229:Dithiothreitol