Ethanol-induced changes in the membrane lipid composition of Clostridium thermocellum.
When ethanol is added to the growth medium of Clostridium thermocellum ATCC 27405 and C9, a different membrane composition is observed after the period of growth arrest. Changes in fatty acid composition and some unsaturated, branched hydrocarbons have been monitored by GLC-MS. There is a marked increase in normal and anteiso-branched fatty acids at the expense of isobranched fatty acids and an increase in short and unsaturated fatty acids. Thus, an adaptive response to growth in the presence of ethanol induces a membrane containing fatty acids with lower melting points and produces a more 'fluid' membrane. The suggestion is made that these membrane changes may be maladaptive to the performance of C. thermocellum.
D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003013:Clostridium / Q000187:drug effects / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids / Q000302:isolation & purification / Q000378:metabolism*
D005227:Fatty Acids; D008563:Membrane Lipids; D000431:Ethanol