Synthesis of [2H8] estradiol, [2H7] estrone, [2H6] 2-hydroxyestrone and [2H6] 4-hydroxyestrone as internal standards for selected ion monitoring.
The synthesis of [2H8] estradiol, [2H7] estrone, [2H6] 2-hydroxyestrone and [2H6] 4-hydroxyestrone from estrone (as a source) is described. The high isotopical purity renders the labelled compounds as suitable carriers and internal standards for quantitative gas chromatography - mass spectrometry. The content of protonium-form (i.e. natural) estrogens in the labelled derivatives ranged from 0.12% to 2.58%. The performance of these compounds in quantitative assays using selected ion monitoring has been established; and this allows the determination of estrogens from biological material in the lower picogram range.
D003903:Deuterium; D004958:Estradiol; D004970:Estrone / Q000031:analogs & derivatives; D008401:Gas Chromatography-Mass Spectrometry; D006894:Hydroxyestrones; D007553:Isotope Labeling / Q000379:methods*; D012015:Reference Standards
D006894:Hydroxyestrones; D004970:Estrone; C026418:4-hydroxyestrone; D004958:Estradiol; D003903:Deuterium; C024980:2-hydroxyestrone