GABA dehydrogenase activity in rat brain.
The crude mitochondrial fraction of rat brain contains an active dehydrogenase involved in the direct oxidation of gamma-aminobutyric acid. INT (p-iodonitrotetrazolium violet) can serve as an efficient acceptor of electrons in this dehydrogenase reaction. During this oxidation of GABA, ammonia is not produced. In vitro the dehydrogenase activity is inhibited by certain MAO inhibitors. The effects of various inhibitors of GABA-T and GAD were also investigated. The dehydrogenase activity was found to be susceptible to various anti-convulsants and inhibitors of electron transport. The co-factors which may be involved in the transfer of electrons during GABA oxidation in the presence of INT are also discussed.
D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D001921:Brain / Q000201:enzymology*; D004579:Electron Transport / Q000187:drug effects; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D013778:Tetrazolium Salts / Q000378:metabolism
D000927:Anticonvulsants; D008996:Monoamine Oxidase Inhibitors; D013778:Tetrazolium Salts; C013574:iodonitrotetrazolium; C036322:GABA dehydrogenase; D010088:Oxidoreductases