[Isolation of West Nile virus in the Southern Ukraine].
A virus (strain No. 3266) was isolated from the blood and internal organs of a rook caught in May, 1980, in the territory of the Black Sea State Preserve of the Ukrainian Academy of Sciences (the Kherson region). The virus is pathogenic for 1--2-day-old mice by various routes of inoculation and for 3-week-old mice by the intracerebral route; it multiplies in chick embryos, in chick embryo fibroblast cultures without the cytopathic effect, and in continuous L cells and pig embryo kidney cells with definite cytopathic effect. The virus contains RNA and has a lipid-containing membrane; according to the results of filtration through Millipore filters, its size ranges from 50 to 100 nm. From the results of immunofluorescent studies and serological identification this strain has been classified as West Nile virus. The characteristics of the biological properties of this virus isolated in the Ukraine have first been described.
D000818:Animals; D001717:Birds / Q000382:microbiology*; D014455:Ukraine; D014902:West Nile virus / Q000302:isolation & purification*
Vydelenie virusa Zapadnogo Nila na iuge Ukrainskoĭ SSR.