Unscheduled DNA synthesis induced in human lymphocytes by butyl glycidyl ethers.
Unscheduled DNA synthesis (UDS) was examined in normal human peripheral blood lymphocytes (HPBL) exposed to n- and tert.-butyl glycidyl ethers (BGE). n-BGE, which is currently used as a major reactive diluent for industrial epoxy resin systems, has been implicated as a bacterial mutagen. t-BGE, a potential replacement for n-BGE, is less cytotoxic to HPBL. Both these BGEs have demonstrated genetic activity in human cells. Here, slide autoradiography and liquid scintillation counting techniques have been used to show that both n-BGE and t-BGE induce repair-DNA synthesis in normal HPBL. These agents demonstrate similar dose-response relationships over the range of concentrations up to 333 micrograms/ml. Cultures were examined for repair-DNA synthesis after a continuous 5-h exposure to varying concentration of n-BGE or t-BGE.
D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004852:Epoxy Compounds / Q000494:pharmacology*; D004988:Ethers, Cyclic / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D009153:Mutagens
D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D009153:Mutagens; C022794:n-butyl glycidyl ether; C036354:tert-butyl glycidyl ether; D004247:DNA