Polypeptide composition of thylakoid membranes: two-dimensional gel analysis during development of Euglena chloroplasts.
Two-dimensional gel electrophoresis is used to analyse the polypeptide composition of thylakoid membranes during chloroplast development in Euglena gracilis. The number of polypeptide spots resolved on the two-dimensional gels is 3-4 times the number of bands previously resolved on one-dimensional gels. Groups of multiple polypeptides with the same Mr but with differing isoelectric points are detected throughout the period of chloroplast development. The pattern of polypeptide synthesis and insertion into the forming thylakoid membranes of dark-grown Euglena exposed to light conforms with previous biochemical measurements on the assembly of the electron transport chain located in these membranes.
D002736:Chloroplasts / Q000502:physiology*; D004579:Electron Transport; D004591:Electrophoresis, Polyacrylamide Gel; D005055:Euglena / Q000502:physiology*; D007425:Intracellular Membranes / Q000032:analysis*; D007700:Kinetics; D008565:Membrane Proteins / Q000032:analysis* / Q000096:biosynthesis; D010455:Peptides / Q000032:analysis*
D008565:Membrane Proteins; D010455:Peptides