Evidence against co-storage of enkephalins with noradrenaline in bovine adrenal medullary granules.
Bovine adrenal chromaffin granules were purified and separated into adrenaline (A) and noradrenaline (NA) containing granule populations by a combination of differential and isopycnic gradient centrifugation. The distribution of [Leu5]enkephalin as measured by radioimmunoassay followed a pattern identical to adrenaline but not noradrenaline on centrifugation. This suggests that enkephalins are stored in the same type of granule where adrenaline is found or that enkephalins and adrenaline even coexist in one and the same granule. Enkephalins may therefore function as co-transmitters or co-hormones of adrenaline rather than noradrenaline in the adrenal medulla.
D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002837:Chromaffin Granules / Q000378:metabolism*; D002838:Chromaffin System / Q000378:metabolism*; D004743:Enkephalin, Leucine / Q000378:metabolism; D004745:Enkephalins / Q000378:metabolism*; D004837:Epinephrine / Q000378:metabolism; D066298:In Vitro Techniques; D009638:Norepinephrine / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
D004745:Enkephalins; D004743:Enkephalin, Leucine; D009638:Norepinephrine; D004837:Epinephrine