title: The behavior of subcultivated stratified squamous epithelial cells on reconstituted extracellular matrices: initial interactions.
abstract: Stratified squamous epithelial cells derived from the ventral surface of the rat tongue interact with matrices composed of hydrated dermal collagens (types I and III) and with a bovine basement membrane in distinctly different ways. When compared with the behavior of the cells on the basement membrane, the attachment and subsequent migration of the cells on the collagens were inefficient and slow. The resultant epithelial colonies did, however, stratify and differentiate more rapidly than those formed on the membrane. The fibrillar meshwork of the type III collagen gel provided better initial support for the cells than did the gel of type I collagen in which the fibrils were arranged as coarse aggregates. The physical characteristics of the type III gel also allowed the cells to condense the surface resulting in even better epithelial support. The basement membrane encouraged rapid epithelial coverage of large areas by promoting cell attachment and migration. This important property indicates that epithelial discontinuities can be rapidly repaired, providing the cells can migrate along a preexistent basement membrane. The use of such defined extracellular matrices in culture can provide important insight into the function and structural organization of subepithelial connective tissues and basement membranes.
mesh_terms: D000818:Animals; D001485:Basement Membrane; D002417:Cattle; D002448:Cell Adhesion; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured / Q000166:cytology*; D002999:Clone Cells / Q000166:cytology; D003094:Collagen; D004847:Epithelial Cells; D005782:Gels; D051381:Rats; D014059:Tongue
chemical_list: D005782:Gels; D003094:Collagen