Zinc-dependent affinity chromatography of the S100b protein on phenyl-Sepharose. A rapid purification method.
A rapid high resolution method of purification of the Trp-containing S100 proteins (S100a, S100a') and of the S100b protein has been developed. The principle of this method is based on the fact that S100b protein becomes highly hydrophobic upon Zn2+ binding, whereas S100a and S100a' are not affected. On an affinity chromatography of phenyl-Sepharose column. S100b is selectively bound in presence of zinc, whereas the Trp-containing S100 proteins are quickly eluted. The S100b protein is further eluted with a buffer containing EDTA.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D011485:Protein Binding; D009418:S100 Proteins / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives; D013053:Spectrophotometry; D013329:Structure-Activity Relationship; D015032:Zinc
D009419:Nerve Tissue Proteins; D009418:S100 Proteins; C020927:phenyl-sepharose; D012685:Sepharose; D015032:Zinc