Electron microscopic evidence for scorpion toxin binding to synapses of rat brain cortex.
The venom from the scorpion Centruroides noxius Hoffman was fractionated by Sephadex G-50 gel filtration. The toxic fraction (n.II) was coupled to Sepharose-4B and used for purification of specific horse immunoglobulins. The purified immunoglobulins were linked to ferritin with glutaraldehyde and repurified in the same affinity column. This complex bound to the postsynaptic membrane of rat brain tissue previously exposed to toxin.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D008854:Microscopy, Electron; D051381:Rats; D011950:Receptors, Cholinergic / Q000378:metabolism*; D012604:Scorpion Venoms / Q000378:metabolism*; D015222:Sodium Channels; D013569:Synapses / Q000378:metabolism*; D013570:Synaptic Membranes / Q000378:metabolism
D011950:Receptors, Cholinergic; D012604:Scorpion Venoms; D015222:Sodium Channels; C029574:scorpion toxin receptor