Excellent wavelength-dependence of laser-induced delayed light-emission from mesophyll and bundle-sheath cells of maize.
Mesophyll and bundle-sheath cells from maize (Zea mays) were examined for delayed light-emission. An enzymic procedure was used to isolate the mesophyll and bundle-sheath cells. A tunable dye laser was used at 695-720 nm to excite delayed light-emission. The mesophyll cells show a typical Photosystem-II dependence on excitation wavelength for delayed light-emission, and this emission was eliminated by hydroxylamine and 3-(3,4-dichlorophenyl)-1,1-dimethylurea. The bundle-sheath cells show no delayed light-emission with these excitation wavelengths. Photosystem-I emission was not observed from either cell type, there being no effect of Methyl Viologen on the observed signals.
D002736:Chloroplasts / Q000528:radiation effects; D007834:Lasers; D008027:Light; D010944:Plants / Q000528:radiation effects*; D011523:Protoplasts / Q000528:radiation effects; D013997:Time Factors; D003313:Zea mays / Q000528:radiation effects