title: Effects of high-fat diet on glucose metabolism in isolated pancreatic acini of rats.
abstract: The purpose of the present study was to document the metabolism of glucose and its responsiveness to insulin in isolated pancreatic acini from rats fed either a low- or high-fat diet. The different steps investigated were labeled glucose oxidation, lactate production, hexokinase activity, and glucose transport, which was assessed by using both 3-O-methylglucose and 2-deoxyglucose. The acinar capacity to metabolize glucose (sum of CO2 plus lactate) was decreased by 50% by feeding the rats a high-fat diet. The impairment of glucose metabolism could not be explained by a defect in the glucose phosphorylation step because hexokinase activity was not changed in isolated acini from rats fed a high-fat diet. The effect of a high-fat diet was entirely accounted for by a reduction in the glucose transport rate that was achieved through a decrease in glucose transport Vmax with no change in Km. We could not detect any effect of insulin on glucose metabolism or 2-deoxyglucose uptake, whatever the diet composition. This work establishes that a high-fat diet, known to markedly alter pancreatic exocrine enzymes, also induces a large decrease in acinar glycolytic flux, raising the question of the relation between these two sets of adaptive changes.
mesh_terms: D019325:3-O-Methylglucose; D000818:Animals; D001693:Biological Transport, Active; D004041:Dietary Fats / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008757:Methylglucosides / Q000378:metabolism; D010179:Pancreas / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004041:Dietary Fats; D008757:Methylglucosides; D019325:3-O-Methylglucose; D006593:Hexokinase; D005947:Glucose