[Kinetics of oxygen consumption, luminescence of pyridine nucleotides and the cyanine dye 3',3'-diethylthiodicarbocyanine iodide after energizing Ehrlich ascites carcinoma cells with glucose].
It has been shown that in the absence of inhibitors of oxidative phosphorylation, changes in fluorescence of 3',3'-diethylthiodicarbocyanine iodide (dis-C2-/5/) in a cell suspension of Ehrlich's ascites carcinoma reflect those in the transmembrane mitochondrial potential. Addition of glucose to the cells in the presence of respiratory inhibitors similar to rotenon induces oscillations in the membrane mitochondrial potential due to H+-ATPase that uses glycolytic ATP. The described changes in energy metabolism parameters are determined by impairment of the interplay between glycolysis and oxidative phosphorylation. The low rate of cytoplasmic NADH oxidation by tumor cell mitochondria favors the latter's accumulation by the cytoplasm and glycolysis inhibition. Pyruvate has been shown to be responsible for NADH oxidation and to remove glycolysis inhibition.
D000818:Animals; D002232:Carbocyanines; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002478:Cells, Cultured; D004734:Energy Metabolism / Q000187:drug effects*; D005456:Fluorescent Dyes; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics; D008163:Luminescent Measurements; D051379:Mice; D009840:Oligomycins / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects*; D011742:Pyrimidine Nucleotides / Q000378:metabolism*; D011804:Quinolines; D012402:Rotenone / Q000494:pharmacology
D002232:Carbocyanines; D005456:Fluorescent Dyes; D009840:Oligomycins; D011742:Pyrimidine Nucleotides; D011804:Quinolines; D012402:Rotenone; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D005947:Glucose
Kinetika potrebleniia kisloroda, liuminestsentsii piridinnukleotidov i tsianinovogo krasitelia 3',3'-dietiltiodikarbotsianin-ĭodida pri énergizatsii kletok astsitnoĭ kartsinomy Erlikha gliukozoĭ.