Histopathological subgrouping of WHO II urothelial neoplasms by cytophotometric measurements of nuclear atypia.
The grade of nuclear atypia was objectively assessed in 21 cases of non-invasive WHO II transitional cell bladder neoplasm. Measurements were performed by stage scanning absorbance cytophotometry, registering nuclear optical density, nuclear area, and the variability of these two factors, in 5 microns thick Feulgen-stained paraffin sections. The material was subgrouped into a 2- and a 2+ group according to the degree of histopathological atypia. Cytophotometrically determined atypia showed close correlation to the subjectively judged atypia, and there was no overlap between 2- and 2+. A difference in tumour ploidy level between different WHO II tumours is the most likely explanation of the grouping recorded.
D002295:Carcinoma, Transitional Cell / Q000145:classification / Q000473:pathology* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure*; D006801:Humans; D007620:Karyometry; D009367:Neoplasm Staging; D010783:Photometry / Q000379:methods; D001749:Urinary Bladder Neoplasms / Q000145:classification / Q000473:pathology* / Q000648:ultrastructure; D014944:World Health Organization