2'5'-adenylate inhibition of erythropoietin-dependent colony formation.
The tetramer core of 2'5'-adenylate was tested in vitro for its effect on erythroid colony-forming units (CFU-E) from bone marrow of adult rats. Inhibition of CFU-E was 2'5'-adenylate dose dependent, when tested with concentrations of 0.05- 1.0 mM. Also, 2'5'-adenylate was inhibitory when incubated with 2, 6 or 10 X 10(4) cells/0.1 ml plasma clot. At 0.1 mM concentration of 2'5'-adenylate there was a 30-50% reduction of erythroid colonies for each of these three cell concentrations. There was an early decrease in 3H-thymidine incorporation when 2'5'-adenylate was added to erythropoietin-stimulated bone marrow cell suspensions prior to plating. It is suggested that in situ production of 2'5'-adenylate may represent a regulatory mechanisms in red cell maturation.
D000227:Adenine Nucleotides / Q000494:pharmacology*; D000818:Animals; D003114:Colony-Forming Units Assay; D004920:Erythropoiesis / Q000187:drug effects*; D004921:Erythropoietin / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D009841:Oligonucleotides / Q000494:pharmacology*; D009843:Oligoribonucleotides / Q000494:pharmacology*; D051381:Rats
D000227:Adenine Nucleotides; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D004921:Erythropoietin; C023505:2',5'-oligoadenylate