Effects of vitamin E deficiency and nitrogen dioxide exposure on lung lipid peroxidation: use of lipid epoxides and malonaldehyde as measures of peroxidation.
The effect of vitamin E deficiency in male Sprague-Dawley rats upon lipid peroxidation in lung tissue was examined by measuring malonaldehyde and lipid epoxide production. In addition to controls, some animals were also exposed to 3 +/- 0.1 ppm NO2 continuously for 7 d in order to study the effects of oxidant stress on lung lipid peroxidation and vitamin E content. The observed changes in malonaldehyde and epoxide content could serve as good indices of lipid peroxidation, particularly under conditions of vitamin E deprivation. The responses measured indicated an inverse relation in the lung between tissue vitamin E content and quantity of lipid peroxidation products. Measurement of lipid epoxides served as a reliable indicator of lung tissue lipid peroxidation. Finally, NO2 inhalation appeared to elicit a response characterized by increased assimilation of vitamin E into lung tissue.
D000818:Animals; D002784:Cholesterol / Q000032:analysis; D004852:Epoxy Compounds / Q000032:analysis*; D004988:Ethers, Cyclic / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D008054:Lipid Peroxides / Q000378:metabolism*; D008055:Lipids / Q000032:analysis*; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008314:Malonates / Q000032:analysis*; D008315:Malondialdehyde / Q000032:analysis*; D009585:Nitrogen Dioxide / Q000494:pharmacology*; D010743:Phospholipids / Q000032:analysis; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000032:analysis; D014810:Vitamin E / Q000032:analysis; D014811:Vitamin E Deficiency / Q000378:metabolism*
D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D005227:Fatty Acids; D008054:Lipid Peroxides; D008055:Lipids; D008314:Malonates; D010743:Phospholipids; D014280:Triglycerides; D014810:Vitamin E; D008315:Malondialdehyde; D002784:Cholesterol; D009585:Nitrogen Dioxide