One-step purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-Gel blue chromatography.
Monoclonal antibodies can be purified directly from ascitic fluids by chromatography on a DEAE Affi-gel blue column. Optimal conditions were determined for the recovery of immunoglobulins free of contaminating protease and nuclease activities.
D000818:Animals; D000873:Anthracenes; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001202:Ascitic Fluid / Q000032:analysis*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006863:Hydrogen-Ion Concentration; D008722:Methods; D051379:Mice; D014227:Triazines
D000873:Anthracenes; D000911:Antibodies, Monoclonal; D014227:Triazines; C012842:Cibacron Blue F 3GA