Formation and catalytic activity of high molecular weight soluble polymers produced by heating amino acids in a modified sea medium.
Eighteen protein amino acids with milk casein composition were heated in a modified sea medium. Marigranules were formed in the precipitates and soluble polymers were formed in the supernatant. Time course of the reaction (ultraviolet spectra, the concentration of metal ions, and the concentration of amino acids in the supernatant) were measured. The time course of the formation of the soluble polymers was also studied by Bio-Gel P-2 column. High molecular weight soluble polymers (HMWSP) were separated from low molecular weight ones by dialysis. It was shown that these polymers catalyzed the dehydrogenation of NADH. These polymers also catalyzed the coupled reaction between dehydrogenation of NADH and reduction of resazurin. This coupled reaction was accelerated by the light.
D000596:Amino Acids; D001704:Biopolymers; D002364:Caseins; D055598:Chemical Phenomena; D002621:Chemistry; D046911:Macromolecular Substances; D008970:Molecular Weight; D012623:Seawater; D013053:Spectrophotometry
D000596:Amino Acids; D001704:Biopolymers; D002364:Caseins; D046911:Macromolecular Substances