Depressed natural and lectin-dependent cell-mediated cytotoxicity against adherent HEp-2 cells in patients with systemic lupus erythematosus.
Natural cell-mediated cytotoxicity /NCMC/ was evaluated using human adherent 3H-thymidine-prelabelled HEp-2 epipharynx carcinoma cells as targets at 50:1 effector-target cell ratio in a 24 hr assay. For lectin-dependent cell-mediated cytotoxicity /LDCC/ studies cultures contained also 25/micrograms/ml concanavalin A /Con A/. Peripheral blood mononuclear cells /PBMC/ of nine patients with active systemic lupus erythematosus /SLE/ failed to exert NCMC or LDCC against HEp-2 targets. In contrast, an increased adherence /decreased detachment from the monolayer/ of HEp-2 target cells was observed in the presence of PBMC from SLE patients that was further promoted by the addition of Con A during LDCC assay.
D000293:Adolescent; D000328:Adult; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007694:Killer Cells, Natural / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008875:Middle Aged; D010610:Pharyngeal Neoplasms / Q000276:immunology
D003208:Concanavalin A