In vivo viability of red blood cells stored in CPDA-2.
The marginal viability of erythrocytes stored for 35 days as red blood cell concentrates in citrate-phosphate-dextrose-adenine-one (CPDA-1) was attributed to inadequate nutrient support with adenine and glucose. In an effort to improve the viability of red blood cells following extended storage, a new CPD-adenine and 1.4 times more glucose than CPDA-1. The efficacy of CPDA-2 was evaluated in vivo by measurement of 24-hour postinfusion recovery of 51Cr-labeled erythrocytes which had been stored as whole blood or red blood cell concentrates for 5 to 8 weeks. All red blood cell concentrates, and the whole blood units stored for 35 and 42 days, were held at room temperature for 8 hours prior to processing and/or refrigeration. CPDA-2 yielded significantly higher 51Cr survivals than CPDA-1 and exceeded the accepted criterion for anticoagulant preservative efficacy of 70 percent postinfusion survival of red blood cells after storage for a period of 42 days. Preliminary data supports possible usage to 49 days. Plasma glucose and red blood cell ATP concentration were maintained better in CPDA-2 than in CPDA-1. When compared to historical controls for CPD and CPDA-1 the data suggest that red blood cells stored in CPDA-02 will have superior viability throughout the entire storage period. CPDA-2 is a candidate to replace CPDA-1 as the anticoagulant-preservative solution of choice for red blood cell concentrates.
D000225:Adenine / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000925:Anticoagulants; D001786:Blood Glucose / Q000378:metabolism; D001793:Blood Preservation; D002951:Citrates / Q000494:pharmacology*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004905:Erythrocyte Aging / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D010710:Phosphates / Q000494:pharmacology*
D000925:Anticoagulants; D001786:Blood Glucose; C034876:CPDA solutions; D002951:Citrates; D003451:Cryoprotective Agents; D010710:Phosphates; D000255:Adenosine Triphosphate; D005947:Glucose; D000225:Adenine