A simple photometric microassay for the quantitative evaluation of macrophage-mediated cytotoxicity on adherent cancer cells.
A new in vitro microassay was devised for the quantitation of macrophage-mediated cytotoxicity on cancer cells. After 72 h of culture with macrophages, the residual adherent target cells were stained with methylene blue. The cell-bound dye was then eluted and measured in a photometer. This assay is simple, quickly performed and gives reproducible results in good correlation with direct counting of the residual cells. It allows a quantitative evaluation of the whole toxic effect (cytostasis and cytolysis) of activated macrophages on adherent tumour cells.
D000818:Animals; D002448:Cell Adhesion; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D007118:Immunoassay / Q000379:methods; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D008751:Methylene Blue / Q000494:pharmacology; D009374:Neoplasms, Experimental / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains
D008751:Methylene Blue