[Isolation, structure elucidation and synthesis of a hexapeptide from the hemodialysate of uremic patients].
Lyophilised hemodialysates of uremic patients were fractionated on Sephadex G-15 for investigation of potential uremic toxins. One of the oligopeptides found in the fraction of postulated toxins was purified to homogeneity by gel, reversed-phase and adsorption chromatography and by high-performance liquid chromatography. Dansyl Edman degradation revealed the sequence Ala-Phe-Phe-Gly-Glu, which was synthesized by the solid-phase method. The comparison of synthetic and natural hexapeptide by chromatographic methods, mass spectrometry, 1H- and 13C-nuclear magnetic resonance proved the correct sequence. The peptide showed only weak activity in immunological test systems and in the lymphocyte proliferation test.
D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008564:Membrane Potentials; D009842:Oligopeptides / Q000097:blood* / Q000138:chemical synthesis; D006435:Renal Dialysis; D014118:Toxins, Biological; D014511:Uremia / Q000097:blood*
D009842:Oligopeptides; D014118:Toxins, Biological; C026865:uremia middle molecule toxins
Isolierung, Strukturaufklrung und Synthese eines Hexapeptids aus dem Hämodialysat urämischer Patienten.