title: Genetic analysis of human lymphocyte proteins by two-dimensional gel electrophoresis: 4. Genetic polymorphism of cytosol 100k polypeptide.
abstract: We describe a genetic polymorphism of a human cellular polypeptide with mol. wt. 100,000, detected in peripheral blood lymphocytes by high resolution two-dimensional electrophoresis. Three different electrophoretic types (1-1, 2-1, and 2-2) of the polypeptide have been identified. Family and population studies indicate that the three phenotypes of the polypeptide are determined by two common alleles at a single autosomal locus. The polypeptide occurs in the cytosol and is one of the abundant polypeptides of B-lymphoblastoid cells, T-lymphoblastoid cells, fibroblasts, and HeLa cells. The data indicate that the cytosol polypeptide with mol. wt. 100,000 shows a genetic polymorphism determined by a new autosomal locus. It is proposed that the polypeptide and its locus be temporarily designated cytosol 100k polypeptide (C100k polypeptide) and C100P, respectively. In a Japanese population, the gene frequencies of C100P1 and C100P2 were 0.907 and 0.093, respectively.
mesh_terms: D000483:Alleles; D001797:Blood Protein Electrophoresis / Q000379:methods; D001798:Blood Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D003600:Cytosol / Q000032:analysis; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008214:Lymphocytes / Q000032:analysis* / Q000187:drug effects; D008297:Male; D008970:Molecular Weight; D010455:Peptides / Q000235:genetics; D010641:Phenotype; D010835:Phytohemagglutinins / Q000494:pharmacology; D011110:Polymorphism, Genetic; D013268:Stimulation, Chemical
chemical_list: D001798:Blood Proteins; D010455:Peptides; D010835:Phytohemagglutinins