The content of coenzyme A, acetyl-CoA and long-chain acyl-CoA in human blood platelets.
The amounts of coenzyme A (CoASH) and acetyl-CoA in the acid-soluble extract of human blood platelets were quantitated by an isocratic reversed-phase liquid-chromatographic system. The analytical column was Supelcosil LC-18, and the solvent was 220 mmol/l potassium phosphate, pH 4.0, and 120 ml/l methanol. Long-chain acyl-CoA was estimated by the released coenzyme A after an alkaline hydrolysis of the acid-insoluble material of the blood platelets. The median values in platelets from fourteen healthy persons were 30 pmol CoASH/mg platelet protein, 45 pmol acetyl-CoA/mg platelet protein, and 34 pmol long-chain acyl-CoA/mg platelet protein.
D000105:Acetyl Coenzyme A / Q000097:blood*; D000214:Acyl Coenzyme A / Q000097:blood*; D001792:Blood Platelets / Q000032:analysis*; D002332:Carnitine O-Acetyltransferase; D002851:Chromatography, High Pressure Liquid; D003065:Coenzyme A / Q000097:blood*; D006801:Humans; D010709:Phosphate Acetyltransferase
D000214:Acyl Coenzyme A; D000105:Acetyl Coenzyme A; D002332:Carnitine O-Acetyltransferase; D010709:Phosphate Acetyltransferase; D003065:Coenzyme A