Localization of bombesin and GRP (gastrin releasing peptide) sequences in gut nerves or endocrine cells.
Five anti-gastrin releasing peptide (GRP) sera have been characterized against GRP, bombesin and related polypeptides spotted on cellulose acetate discs. Antibodies reacting with the C-terminal G-14 sequence of bombesin and the 19-27 sequence of GRP, were detected in all sera. Antibodies directed exclusively against the bombesin unrelated 1-17 sequence of GRP were found only in one serum (R-6902). With parallel immunohistochemical tests only the C-terminal immunoreactivity was detected in endocrine-paracrine cells of the chicken proventriculus, while both immunoreactivities were present in nerve fibres and a few nerve cell bodies of the mammalian gut. The distribution of GRP- and bombesin-like immunoreactive nerves in the gastric mucosa of both pyloric and oxyntic type the submucosal and myenteric plexus along the whole gastrointestinal wall and at sphincter regions is detailed.
D000818:Animals; D001839:Bombesin / Q000032:analysis*; D002645:Chickens; D004064:Digestive System / Q000294:innervation*; D004702:Endocrine Glands / Q000032:analysis*; D019886:Gastrin-Releasing Peptide; D006168:Guinea Pigs; D006651:Histocytochemistry; D007120:Immunochemistry; D010455:Peptides / Q000032:analysis*; D051381:Rats; D013045:Species Specificity; D013552:Swine / Q000276:immunology
D010455:Peptides; D019886:Gastrin-Releasing Peptide; D001839:Bombesin