Simple method for the screening of polymeric Bence Jones proteins.
The author tested the electropherograms of previously identified, concentrated urine samples in agarose gel. Before electrophoresis, the gel plate containing the samples was covered with a cellulose-acetate membrane soaked with 0.1 M 2-mercaptoethanol, and it was incubated for 10 min in a moist chamber, at room temperature. If the polymeric Bence Jones protein is present, the electropherogram of the sample changes. In the following, a quick and easy method of recognizing this rare structural anomaly is recommended.
D001536:Bence Jones Protein / Q000652:urine*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008623:Mercaptoethanol / Q000494:pharmacology; D010954:Plasmacytoma / Q000175:diagnosis
D008623:Mercaptoethanol; D001536:Bence Jones Protein