Fine structural studies of ciliary processes after treatment with cholera toxin or its B subunit.
Delivery of 2 micrograms of cholera toxin (CT), a specific, irreversible activator of adenyl cyclase, via the blood causes dilation of capillaries and stromal edema of the ciliary processes. These morphologic changes occur within 3 h, are maximal at 12 to 24 h, then gradually return to normal by 72 h. In the late phase of hypotony, ultrastructural changes in the ciliary epithelia, similar to Greeff vesicles, are due to a "paracentesis effect" from hypotony, caused by decreased aqueous flow through the eye. Delivery of 2 micrograms of the B subunit of CT (Sub-B) causes very mild capillary dilation and stromal edema of ciliary processes. These changes reach their peak at 3 h, then return to normal at 24 h. No significant damage occurred to the pigmented or non-pigmented epithelium with either agent. No hemorrhage, invasion of inflammatory cells or appearance of fibrin exudates in the ciliary processes could be detected.
D000818:Animals; D002772:Cholera Toxin / Q000008:administration & dosage / Q000494:pharmacology*; D002924:Ciliary Body / Q000098:blood supply / Q000187:drug effects* / Q000648:ultrastructure; D006168:Guinea Pigs; D007269:Injections, Intra-Arterial; D007413:Intestinal Mucosa / Q000098:blood supply; D007429:Intraocular Pressure / Q000187:drug effects; D008297:Male; D008854:Microscopy, Electron; D011817:Rabbits; D051381:Rats; D014664:Vasodilation
D002772:Cholera Toxin