Successful in vivo experiments in sheep using a new nonhydrolyzable enzyme reactor resistant against tryptic digestion.
Blockage of E-amino groups of asp-ase bound to cellulose by succinylation results in a greatly enhanced resistance against tryptic digestion by "plasma enzymes." Plasmapheresis in sheep by plasma filters connected to asp-ase Cellulose cartridges led to decrease of asp-NH2 and glut-NH2 in plasma over 21 hrs with undetectable enzyme leakage from the solid phase.
D000818:Animals; D001215:Asparaginase / Q000378:metabolism; D005972:Glutaminase / Q000378:metabolism; D006868:Hydrolysis; D010956:Plasmapheresis / Q000379:methods*; D012756:Sheep; D014357:Trypsin / Q000494:pharmacology*
D014357:Trypsin; D001215:Asparaginase; D005972:Glutaminase