Cross-linked stroma-free polyhemoglobin as a potential blood substitute.
Stroma-free hemoglobin is prepared from human blood and then cross-linked with glutaraldehyde to produce soluble polyhemoglobin. Results from animal studies show that in the case of polyhemoglobin, its level in the circulation remained unchanged at 77% of the injected amount when followed for 3 hours; whereas stroma-free hemoglobin was removed rapidly with a half-life of 87 minutes. Thus, cross-linking stroma-free hemoglobin into the polyhemoglobin form has solved the major problem of rapid removal of stroma-free hemoglobin from the circulation.
D000818:Animals; D001802:Blood Substitutes / Q000008:administration & dosage / Q000627:therapeutic use*; D006454:Hemoglobins / Q000008:administration & dosage / Q000378:metabolism / Q000627:therapeutic use*; D006801:Humans; D007269:Injections, Intra-Arterial; D007275:Injections, Intravenous; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D001802:Blood Substitutes; D006454:Hemoglobins; C037158:polyhemoglobin