Gas chromatographic determination of bupivacaine in plasma using a support coated open tubular column and a nitrogen-selective detector.
A capillary gas chromatographic method for the quantitative determination of bupivacaine in plasma is described. Bupivacaine and an added internal standard were extracted from plasma with n-pentane. The extracted products were introduced into the gas chromatograph via a solid injection system. The combination of a support coated open tubular column and a nitrogen-selective detector insure a good selectivity and a high sensitivity. The coëfficient of variation at concentrations exceeding 3 ng bupivacaine/ml plasma is less than 6%. The detection limit is about 1 ng/ml.
D002045:Bupivacaine / Q000097:blood*; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods; D006801:Humans; D009584:Nitrogen / Q000032:analysis
D009584:Nitrogen; D002045:Bupivacaine