title: Ultrastructural study of seminiferous tubules in the rat after prenatal irradiation.
abstract: Is the presence of germinal cells necessary for the Sertoli cells to acquire normal features? To respond to this question we have studied the development of the Sertoli cells in rats irradiated at the end of the foetal life. In the prenatal irradiated rats, the lumen of the seminiferous tubules appears later than in the control rats. The Sertoli cells show numerous flexuose apical processes, with central microtubule bundles. These processes regress progressively after the 40th day of life when the tubular lumen appears; numerous junctional complexes differentiate with the same structure as those of control animals. There are important dilatations of the intercellular spaces. The cytoplasmic organelles show a normal development up to the 40th day of life. After this period, the rough endoplasmic reticulum and the Golgi apparatus clearly regress while important dilatations appear in the smooth endoplasmic reticulum and persist in the adult animal. From the 35th day on, the basal lamina of the seminiferous tubules is irregular and multilayered. The differentiation of the Sertoli cells seems to be independent of the presence of germinal cells until the 40th day of life and presents several particularities; thereafter the Sertoli cells show signs of regression.
mesh_terms: D000818:Animals; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005260:Female; D006056:Golgi Apparatus / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D008870:Microtubules / Q000648:ultrastructure; D011247:Pregnancy; D051381:Rats; D012671:Seminiferous Tubules / Q000254:growth & development / Q000528:radiation effects* / Q000648:ultrastructure; D012708:Sertoli Cells / Q000528:radiation effects / Q000648:ultrastructure; D013737:Testis / Q000528:radiation effects*; D013997:Time Factors