3-phenylazo-4-hydroxyphenylisothiocyanates: reagents for efficient haptenation of immunoglobulin and other carrier molecules.
The synthesis of 2 non-cross-reacting 3-(substituted-phenyl)-azo-4-hydroxyphenylisothiocyanates is described. These compounds are designed as alternatives to the corresponding hydroxybenzimidates for use in hapten-sandwich double labelling of cell surfaces. The new reagents rapidly and controllably haptenate immunoglobulin and other biological carrier molecules. Haptenated immunoglobulins present the appropriate hapten determinant with little concomitant loss of antigen binding activity.
D000818:Animals; D001391:Azo Compounds; D001666:Binding Sites, Antibody; D002352:Carrier Proteins / Q000276:immunology; D002417:Cattle; D005260:Female; D006041:Goats; D006241:Haptens / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007136:Immunoglobulins / Q000276:immunology*; D017879:Isothiocyanates; D008297:Male; D011817:Rabbits; D051381:Rats; D013861:Thiocyanates / Q000494:pharmacology*
D001391:Azo Compounds; D002352:Carrier Proteins; D006241:Haptens; D007074:Immunoglobulin G; D007136:Immunoglobulins; D017879:Isothiocyanates; D013861:Thiocyanates; C013645:4-phenylazophenylisothiocyanate