A photo-CIDNP study of the active sites of Megasphaera elsdenii and Clostridium MP flavodoxins.
Megasphaera elsdenii and Clostridium MP flavodoxins have been investigated by photo-CIDNP techniques. Using time-resolved spectroscopy and external dyes carrying different charges it was possible to assign unambiguously the resonance lines in the NMR-spectra to tyrosine, tryptophan and methionine residues in the two proteins. The results show that Trp-91 in M.elsdenii and Trp-90 in Cl.MP flavodoxin are strongly immobilized and placed directly above the benzene subnucleus of the prosthetic group. The data further indicate that the active sites of the two flavodoxins are extremely similar.
D001665:Binding Sites; D003013:Clostridium / Q000378:metabolism*; D005418:Flavodoxin / Q000378:metabolism*; D005420:Flavoproteins / Q000378:metabolism*; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D013045:Species Specificity; D045854:Veillonellaceae / Q000378:metabolism*
D005418:Flavodoxin; D005420:Flavoproteins