title: Histamine-receptor bearing lymphocytes. II. The use of flow cytofluorometric analysis of histamine-receptors on mouse lymphocytes of different organs.
abstract: The histamine binding capacity of cells in various lymphoid organs has been determined by flow cytofluorometric (FCF) analysis of histamine receptors. Using fluoresceinated bovine serum albumin conjugated to histamine (HIS-BSA-FITC) as a staining reagent two distinct lymphocyte subpopulations of differing fluorescence intensity have been identified in each organ investigated. The thymus has a low number of histamine-receptor bearing cells, while bone marrow, lymph nodes and spleen reveal increasing binding capacities to the histamine conjugate. It is suggested that there exists a different susceptibility of distinct lymphocyte subpopulations for histamine, thereby enabling its controlled immunoregulatory activity.
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D005434:Flow Cytometry; D007700:Kinetics; D008198:Lymph Nodes / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011968:Receptors, Histamine / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism
chemical_list: D011968:Receptors, Histamine; D012710:Serum Albumin, Bovine