An improved method for performance of the C1 fixation and transfer test.
A modification of the traditional C1 fixation and transfer test of Borsos and Rapp is presented. The modified method separates C1 bound to antibody in the fixation stage from free C1 by centrifugation of antigen-antibody-C1 complexes through silicone oil or albumin. This provides a rapid and complete separation of bound and free C1 so that the fixation and transfer test becomes quicker and easier. For high affinity antibodies, the modified technique gives equivalent data to the traditional method. For some low avidity antibodies or unstable surfaces, the traditional technique is unable to measure bound C1, but the C1 fixation and transfer test can be performed successfully by the modified method.
D000818:Animals; D000915:Antibody Affinity; D003172:Complement C1; D003168:Complement Fixation Tests / Q000379:methods*; D005577:Forssman Antigen / Q000276:immunology; D006168:Guinea Pigs; D006461:Hemolysis; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D011817:Rabbits
D003172:Complement C1; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D005577:Forssman Antigen