The detection of serum antibody response to type-B influenza virus in vaccinated individuals by enzyme linked immunosorbent assay.
The sensibility of the Immunoenzymatic ELISA test for the evaluation of antibody response in individuals vaccinated with inactivated Influenza vaccine was evaluated in comparison with the results obtained by the Haemagglutination inhibition test using type B Influenza virus as antigen. The rate of seroconversion obtained with the two methods versus each class of HAI titre or each interval of OD values of the prevaccine sera showed similar features. In 23% of the cases, however, sero-conversion was observed only by the ELISA test, in 5.4% of the cases only by the HAI test. It is to be assessed whether the serological response revealed by the ELISA test is fully correlated with protection against the disease.
D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007252:Influenza Vaccines / Q000276:immunology*; D009975:Orthomyxoviridae / Q000276:immunology*; D014611:Vaccination; D014613:Vaccines, Attenuated
D000914:Antibodies, Viral; D007252:Influenza Vaccines; D014613:Vaccines, Attenuated