Determination of rifampicin, desacetylrifampicin, isoniazid and acetylisoniazid by high-performance liquid chromatography: application to human serum extracts, polymorphonucleocytes and alveolar macrophages.
A method for the determination of rifampicin, desacetylrifampicin, isoniazid, and acetylisoniazid by high-performance liquid chromatography and using the same extract of the same sample is reported. After protein precipitation and extraction of these antituberculous drugs, two reversed-phase chromatographies were necessary. The technique was applied to serum extracts, polymorphonucleocytes and alveolar macrophages from patients treated for tuberculosis.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007538:Isoniazid / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000627:therapeutic use; D008264:Macrophages / Q000032:analysis*; D009504:Neutrophils / Q000032:analysis*; D012293:Rifampin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000627:therapeutic use; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy
C006377:(N)1-acetylisoniazid; C032393:25-deacetylrifampicin; D007538:Isoniazid; D012293:Rifampin