title: A new hexasaccharide chain isolated from bovine colostrum kappa-casein taken at the time of parturition.
abstract: The carbohydrate units linked to caseinoglycopeptide from colostrum taken 30 min after parturition were released as reduced oligosaccharides by alkaline borohydride treatment, and separated into four acidic oligosacchariditols (a hexa- (11.0%), penta- (38.5%), tetra- (35.5%) and a trisacchariditol (15.0%)). Structural studies showed that the hexasacchariditol was a new structure and had the chemical structure: NeuNAc-alpha-2-3-Gal-beta-1-3-[NeuNAc-alpha-2-3-Gal- beta-1-4-GlcNAc-beta-1-6-]-N-acetylgalactosaminitol(GalNAcItol). The other three oligosacchariditols were shown to be NeuNAc-alpha-2-3-Gal-beta-1-3-[Gal-beta-1-4-GlcNAc-beta-1-6-]-GalNAcItol, NeuNAc-alpha-2-3-Gal-beta-1-3-[NeuNAc-alpha-2-6-]-GalNAcItol and NeuNAc-alpha-2-3-Gal-beta-1-3-GalNAcItol, which were identical with those found previously in colostrum kappa-casein taken 6 h after parturition.
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002364:Caseins / Q000302:isolation & purification*; D002417:Cattle; D003126:Colostrum / Q000032:analysis*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007743:Labor, Obstetric; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000302:isolation & purification*; D011247:Pregnancy
chemical_list: D002364:Caseins; D009844:Oligosaccharides