Isolation of an in vitro-produced soluble Anaplasma-albumin complex.
A soluble Anaplasma-albumin complex was isolated from supernatants of A marginale cultures by affinity chromatography. Holstein calves inoculated with this fraction produced anti-A marginale antibodies detected in the indirect fluorescent antibody test at titers similar to those found in cattle immunized with unfractionated culture supernatant. Titers of both groups peaked slightly above 1:2,000 on postinoculation day 46.
D000709:Anaplasma / Q000276:immunology*; D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002417:Cattle / Q000276:immunology; D007841:Latex Fixation Tests; D012710:Serum Albumin, Bovine / Q000276:immunology* / Q000302:isolation & purification
D000942:Antigens, Bacterial; D012710:Serum Albumin, Bovine