title: Flow of endogenous and exogenous amino acids along the gut of pigs.
abstract: Digesta were collected from 5 pigs of 33 kg live weight fitted with re-entrant cannulas in the duodenum (within 20-30 cm of the pylorus) and terminal ileum. The pigs received a diet of barley, soya bean oilmeal and a vitamin and mineral mixture. The flow rates of digesta, total nitrogen and the individual amino acids were measured at different time after feeding and during two 24 h periods. A marked increase in the flow of digesta, nitrogen and amino acids was seen in the duodenum after feeding. Total flow during 24 h of nitrogen and amino acids except His, Val, Leu, Phe and Met exceeded intake. Output of nitrogen and amino acids from the duodenal cannula was 117 and 108% of intake, respectively. A method to calculate the ratio of endogenous amino acids in digesta based on the amino acid composition of digesta, diet and endogenous secretions was developed. The calculated amounts of endogenous amino acids passing the proximal duodenum and terminal ileum were 32.2 and 21.9 g per 24 h, respectively. The greatest amount of endogenous amino acids passed through the duodenal cannula in the first two hours after feeding (2-3 g/h) and then gradually decreased to 1 g per hour. The results are discussed in relation to other studies on the secretion of endogenous protein and its amino acid composition.
mesh_terms: D000596:Amino Acids / Q000008:administration & dosage / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D002404:Catheterization; D004063:Digestion; D004386:Duodenum / Q000502:physiology; D005769:Gastrointestinal Motility; D007082:Ileum / Q000502:physiology; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000502:physiology*; D008297:Male; D009584:Nitrogen / Q000378:metabolism*; D013552:Swine / Q000502:physiology*
chemical_list: D000596:Amino Acids; D009584:Nitrogen