[Extraction of substances from chromatographic layers on a flexible support in microvolumes of the solvent].
This paper describes a modification of the method of direct elution of substances from chromatographic plates and the method based on preliminary concentration on a small area of the sorbent and its subsequent extraction. These methods can be used for simultaneous extraction of several samples from chromatographic plates on a flexible support in 30-50 microliter of the solvent. The paper discusses the selection of solvent systems to be applied for substance extraction from plates. It also considers advantages and disadvantages of the methods as illustrated by nucleic acid elution from the plates Silufol UF-254 and amino acid elution from the plates Fixion 50X8. The elution of the components reaches 97-99%. The process can be performed in routine laboratory glassware.
D002855:Chromatography, Thin Layer / Q000295:instrumentation / Q000379:methods*; D009696:Nucleic Acids / Q000302:isolation & purification; D012997:Solvents / Q000494:pharmacology*
D009696:Nucleic Acids; D012997:Solvents
Izvlechenie veshchestv s khromatograficheskikh sloev na gibkoĭ podlozhke v mikroob"emakh rastvoritelia.