title: Coding assignments of the five smaller mRNAs of Newcastle disease virus.
abstract: The polypeptide coding assignments for the five messengers of the 18S size class of Newcastle disease virus (NDV) RNA have been determined by cell-free translation of individual RNAs separated by gel electrophoresis. Listed in order of their decreasing electrophoretic mobilities in acid agarose-urea gels, the coding assignments of the RNAs were as follows: RNA 1, M protein; RNA 2, P protein; RNA 3, NP; RNA 4, F glycoprotein; and RNA 5, HN glycoprotein. RNA 2 also directed the synthesis of 33- and 36-kilodalton proteins, which were tentatively identified as being overlapping segments of the P protein. The 33- and 36-kilodalton polypeptides could be detected in infected cells, but not in purified virions of NDV. Since the other unique NDV RNA, a 35S species, has been shown previously to encode the viral L protein, these results complete the coding assignments of the six known NDV mRNAs.
mesh_terms: D000818:Animals; D002474:Cell-Free System; D002642:Chick Embryo; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics; D009522:Newcastle disease virus / Q000235:genetics*; D010455:Peptides / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D014357:Trypsin; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D006023:Glycoproteins; D010455:Peptides; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D014357:Trypsin