[Complex electron microscopic cytochemical and stereological study of the action of clofibrate on peroperoxidasosome volume and count in the neutrophilic leukocytes of mouse bone marrow].
The distribution of peroxidase activity in maturing neutrophils of intact mice and of mice treated with clofibrate for a fortnight is studied. The product of peroxidase reaction in neutrophil promyelocytes in experimental and control animals is localized in the perinuclear cisternae, cisternae of RER and Golgi complexo and in primary granules. Peroxidases activity disappears from the secretory apparatus upon the neutrophil maturation. In the mature cells, the product of peroxidase reaction is seen only in the primary granules. Stereological method showed that the volume of peroxidase positive granules in intact and experimental animals decreased by 2.5 times. Clofibrate increases the volume of the primary granules by two times but exerts no effect on the quantity of these organelles.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D002994:Clofibrate / Q000494:pharmacology*; D006651:Histocytochemistry; D008297:Male; D051379:Mice; D008830:Microbodies / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D009940:Organoids / Q000187:drug effects*; D010544:Peroxidases / Q000378:metabolism*
D010544:Peroxidases; D002994:Clofibrate
Kompleksnoe élektronno-tsitokhimicheskoe i stereologicheskoe izuchenie deĭstviia klofibrata na ob"em i kolichestvo peroksidazosom neĭtrofil'nykh leĭkotsitov kostnogo mozga mysheĭ.