Determination of allantoin and chlorohydroxyaluminium allantoinate in cosmetic and pharmaceutical products by high-performance liquid chromatography.
A high-performance liquid chromatographic method is described for convenient and direct determination of allantoin derivatives, by which as little as 0.1 micrograms of the compounds can be determined without any conventional sample preparation required to eliminate interferences. Chromatographic separation on a resin-based strong cation exchanger (H+) using water as eluent is combined with sensitive and selective post-column reaction of the allantoin derivatives. After dissolution of the sample in hot water, the determinations take 12 min per sample. The relative standard deviation is less than 1.8% and quantitative recoveries are obtained. Several commercial cosmetic and pharmaceutical products were analysed successfully. The method can be applied to test the stability of the compounds in formulations.
D000481:Allantoin / Q000031:analogs & derivatives* / Q000032:analysis*; D000536:Aluminum Hydroxide / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003358:Cosmetics / Q000032:analysis*; D009824:Ointments / Q000032:analysis; D011208:Powders / Q000032:analysis; D013488:Suppositories / Q000032:analysis
D003358:Cosmetics; D009824:Ointments; D011208:Powders; D013488:Suppositories; C036141:chlorohydroxyaluminum allantoinate; D000481:Allantoin; D000536:Aluminum Hydroxide