title: [Effect of organic substances on the growth and cobalamin genesis of Achromobacter cobalamini].
abstract: Corn steep and molasses were shown to stimulate cobalaminogenesis in Achromobacter cobalamini. Their concentrations optimal for the culture growth and vitamin B12 synthesis were found. Corn steep at a concentration of 2% accelerated the growth. The biomass yield and the content of B12 were maximal after 48 h of fermentation. In the medium with molasses, the growth was stimulated at 1% of molasses, and the vitamin biosynthesis at 5%. When 5% of molasses was added into the mineral medium, the rate of biosynthesis increased by a factor of 5 to 7 as compared to the control. A 5% aqueous solution of molasses containing 1.1 g/l of potassium phosphates produced the same effect as an addition of molasses to the complete mineral medium.
mesh_terms: D000421:Alcaligenes / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005285:Fermentation / Q000187:drug effects; D014805:Vitamin B 12 / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D014805:Vitamin B 12
vernacular_title: Vliianie organicheskikh veshchestv na rost i kobalaminogenez Achromobacter cobalamini.