Urinary 3-methoxy-4-hydroxyphenylglycol determination using reversed-phase chromatography with amperometric detection.
A reversed-phase liquid chromatographic method with amperometric detection has been developed for the determination of urinary 3-methoxy-4-hydroxyphenylglycol. Before and after enzymatic deconjugation, it was purified by an extraction procedure and rapidly quantified under isocratic conditions. The 24-h excretion profile in normal human subjects (eight males and seven females) was determined; our results are consistent with those arrived at in a number of other studies. The present method is highly sensitive and selective; in addition, a good degree of precision is assured by use of 4-methoxy-3-hydroxyphenylglycol as internal standard.
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods; D004563:Electrochemistry; D005260:Female; D005966:Glucuronidase; D006018:Glycols / Q000652:urine*; D006801:Humans; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000302:isolation & purification / Q000652:urine*; D009097:Multienzyme Complexes; D013429:Sulfatases
D006018:Glycols; D009097:Multienzyme Complexes; C023059:glusulase; D008734:Methoxyhydroxyphenylglycol; D013429:Sulfatases; D005966:Glucuronidase