[A method for the gas chromatographic determination of long and medium chain free fatty acids in serum].
Free fatty acids in serum were determined by gas chromatography after esterification with boron tri-fluoride-methanol. Following prepurification of the serum lipid fractions by thin layer chromatography, the described analytical procedure selectively determines the medium and long chain free fatty acids. Accuracy and precision were tested. The method is suitable for the analysis of fatty acid kinetics in blood, following lipid infusions.
D002849:Chromatography, Gas / Q000379:methods; D002855:Chromatography, Thin Layer; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D006801:Humans; D007700:Kinetics
D005230:Fatty Acids, Nonesterified
Eine Methode zur gaschromatographischen Bestimmung der lang- und mittelkettigen freien Fettsäuren im Serum.