[Species and tissue differences of reparative DNA synthesis in embryonic cell cultures treated with carcinogens].
DNA repair synthesis (RS) was studied in embryonic cell cultures exposed to different carcinogenic factors: UV-light, N-methyl-N-nitro-N-nitrosoguanidine, 4-nitroquinoline-1-oxide, aflatoxin BI and 7,12-dimethylbenz(a)anthracene. DNA RS level was shown to be higher in human liver cells than in murine ones. Tissue-dependent differences in DNA RS of cells damaged by carcinogens were found, too. RS-activity was higher in human, mouse and rat fibroblast cultures than in liver cultures of the same species. RS level in human kidney cultures was similar to that in human fibroblasts. The said differences should be taken into account in the evaluation of the results of testing of chemical agents for carcinogenicity, using their ability to cause DNA repair synthesis.
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002478:Cells, Cultured; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D007668:Kidney / Q000187:drug effects* / Q000528:radiation effects; D008099:Liver / Q000187:drug effects* / Q000528:radiation effects; D051379:Mice; D008809:Mice, Inbred C3H; D009928:Organ Specificity / Q000187:drug effects / Q000528:radiation effects; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D014466:Ultraviolet Rays
D002273:Carcinogens
Vidovye i tkanevye razlichiia reparativnogo sinteza DNK v kul'turakh émbrional'nykh kletok, obrabotannykh kantserogenami.