Lactate dehydrogenase activity in mouse lung following 1,1-dichloroethylene: index of airway injury.
Lactate dehydrogenase (LD) activity and its isozyme profile in mouse lung homogenate was affected by oral administration of 1,1-dichloroethylene (1,1-DCE). Following 100 mg 1,1-DCE/kg, LD-3 increased significantly. After 200 mg 1,1-DCE/kg, LD-5 increased whereas LD-1 and LD-2 decreased, with a resultant higher M:H ratio than controls. In contrast, elevated LD activity in serum following 1,1-DCE was predominantly associated with striking increases in total activity and changes in isozyme patterns resulting in a decrease in the M:H ratio. LD activity in liver and erythrocytes were unaffected by 1,1,-DCE administration. Although total activity in kidney was decreased, no changes were detected in the isozyme profile. Pulmonary damage induced by 1,1-DCE was reflected in significant increases in total activity and all isozymes in bronchopulmonary lavage fluids. Thus, detection of lung-derived LD activity in lung lavage fluids can be a useful index of pulmonary airway injury.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004000:Dichloroethylenes / Q000008:administration & dosage / Q000633:toxicity*; D006843:Hydrocarbons, Chlorinated / Q000633:toxicity*; D007527:Isoenzymes; D007668:Kidney / Q000201:enzymology; D007770:L-Lactate Dehydrogenase / Q000097:blood / Q000378:metabolism*; D008168:Lung / Q000201:enzymology*; D008171:Lung Diseases / Q000139:chemically induced / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
D004000:Dichloroethylenes; D006843:Hydrocarbons, Chlorinated; D007527:Isoenzymes; C029297:vinylidene chloride; D007770:L-Lactate Dehydrogenase