Comparison of methods for the determination of beta-aspartylglycine in fecal supernatants of leukemic patients treated with antimicrobial agents.
Beta-Aspartylglycine is an indicator of the absence of bacterial enzymatic activity in the intestinal tract. This study describes and compares four methods--(1) dansylation with thin-layer chromatography, (2) ion-exchange chromatography, (3) thin-layer electrophoresis, (4) high-voltage paper electrophoresis--to determine the concentration of beta-aspartylglycine in fecal supernatants of leukemic patients treated with antimicrobial agents.
D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D003619:Dansyl Compounds; D004151:Dipeptides / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods; D005243:Feces / Q000032:analysis*; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007938:Leukemia / Q000378:metabolism / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D003619:Dansyl Compounds; D004151:Dipeptides; C018459:beta-aspartylglycine