Intrastrain recurrent idiotypes among anti-DNA antibodies of (NZB x NZW)F1 hybrid mice.
Immunization of NZB and A/J mice against an anti-DNA hybridoma antibody (F227) derived from (NZB x NZW)F1 (B/W) mice allowed the preparation of two anti-idiotypic antisera. These two reagents were shown to recognize different idiotopes of the F227 monoclonal antibody. NZB anti-idiotypic antibodies recognized non-ligand-modifiable idiotypic determinants. These idiotopes were private or present at undetectable level in BW mouse sera since it was found that only two of the 24 B/W mouse sera tested were recognized by these antibodies. Conversely, A/J anti-idiotypic antibodies recognized partially ligand-modifiable idiotopes which were found in all B/W mouse sera tested. These results demonstrate that anti-DNA antibodies share similar idiotypic specificities and suggest that these autoantibodies occur as families of structurally related proteins.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology*; D004247:DNA / Q000276:immunology*; D006824:Hybridization, Genetic; D007074:Immunoglobulin G / Q000276:immunology; D007130:Immunoglobulin Idiotypes; D051379:Mice; D008814:Mice, Inbred NZB; D008815:Mice, Inbred Strains
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D004247:DNA