[Protein assay for cellular receptors of steroids: a critical study of the technic with Coomassie-blue].
The Coomassie-Blue method for protein determination is the EORTC uniformed technique for steroid receptor assay in tumor tissues. Many quality control trials have shown unacceptable interlaboratory variation coefficients for protein determination in steroid receptor assay. The objective of this study is to check the critical parameters of the method. The authors propose some suggestions on stability of dye-protein complex, ionic strength and temperature influence to improve the accuracy of the method.
D000818:Animals; D008722:Methods; D009994:Osmolar Concentration; D011987:Receptors, Steroid / Q000032:analysis*; D012394:Rosaniline Dyes; D013053:Spectrophotometry; D013696:Temperature
D011987:Receptors, Steroid; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue
Dosaggio delle proteine nella determinazione dei recettori cellulari degli steroidi: studio critico della tecnica con Coomassie-blue.