Binding between immobilized anti-colipase purified antibodies and colipase. Radioimmunoassay of colipase from pig plasma and pancreatic juice.
Procedures for purification of porcine colipase II (Gly6-Gly89) and for obtaining purified anti-colipase antibodies are described. The interactions between antibodies immobilized on an Ultrogel AcA 22 column and colipase were investigated and colipase radioimmunoassay carried out. The immobilized antibody-colipase binding was preserved in the presence of mixed micelles, lipase, or both when added to the elution mixture. Bound colipase maintained its capability of interacting with mixed micelles, but not with lipase in either the presence or the absence of mixed micelles. It could be inferred that the antigenic site(s) is independent of the interfacial recognition site and close to the site of lipase recognition. Results are reported suggesting that one or both colipase histidyl residue-containing sequences are involved as antigenic determinant(s). Immunoreactive colipase, bound to a macromolecular protein complex, was found in the plasma of pig. This finding could be explained by an endocrine 'leakage' of colipase from the exocrine pancreatic cell rather than by passage through the intestinal mucosa.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D003089:Colipases / Q000032:analysis* / Q000097:blood / Q000276:immunology; D007700:Kinetics; D008823:Micelles; D010189:Pancreatic Juice / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods; D012756:Sheep / Q000276:immunology; D013552:Swine
D000906:Antibodies; D000936:Antigen-Antibody Complex; D003089:Colipases; D008823:Micelles; D011506:Proteins