[Comparative evaluation of methods of obtaining phagolysates modelled on d'Herelle's plague microbe phage].
Various methods for phage multiplication have been tested: the method of cellophane membranes, the method of combined media, the method of forced aeration of a liquid medium, growth at suboptimal temperature and growth at optimal temperature under stationary conditions. The phage in a high concentration (n X 10(10) plaque-forming units per ml) can be obtained by any of these methods with the exception of the last one yielding lower (by 1 or 2 orders) effectiveness of multiplication. The main factors which determine the titer of the phage in lysate are concentration of live cells in the suspension and temperature of growth.
D001435:Bacteriophages / Q000302:isolation & purification*; D003470:Culture Media / Q000494:pharmacology; D008722:Methods; D013696:Temperature; D014776:Virus Cultivation / Q000379:methods; D015010:Yersinia pestis
D003470:Culture Media
Sravnitel'naia otsenka sposobov polucheniia fagolizatov na modeli chumnoĭ mikrob--fag d'Erellia.