title: Nerve growth factor requirement of postnatal rat adrenal medullary cells in vitro for survival, aggregate formation and maintenance of extended neurites.
abstract: Rat adrenal medullary cells, taken at different postnatal stages (1, 4, 10 and 14 days), were cultured in serum-containing medium with and without nerve growth factor (NGF 2.5 S, 50 ng/ml) to investigate their dependence on NGF for survival and maintenance of extended neurites. It was found that medullary cells depend on NGF for survival at day 1 and, to a lesser extent, at day 4. Neurite-like processes extended in response to NGF treatment breakdown when NGF is withdrawn. NGF-treated adrenal medullary cells displayed a pronounced tendency to form aggregates. It was also shown that plating adrenal medullary cells at higher cell densities, which largely increased the numbers of fibroblast-like cells present, may substitute for NGF with respect to survival.
mesh_terms: D000313:Adrenal Medulla / Q000166:cytology / Q000187:drug effects*; D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D002449:Cell Aggregation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D009414:Nerve Growth Factors / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D009414:Nerve Growth Factors