[Use of polyamine levels in the study of the antitumor activity of natural substances; use of hepatic regeneration as a study model].
The effect of antitumor drugs has been considered according to in vivo variations of the polyamine levels. The experimental model of cellular proliferation is not a tumoral process but a hepatic regeneration in partially hepatectomized normal rats. This study consists of the treatment of regenerating animals with antiproliferative, therefore antitumor natural drugs, such as colchicine, vincaleucoblastine, and 9-methoxy-ellipticine. Tissular free polyamines (putrescine, spermidine, spermine) in the livers of the animals are estimated. Important rate fluctuations are observed and are related, first, to regeneration time, thus to cellular cycle, and, second, to the presumed effect of these natural drugs. Such a study would, first show the antiproliferative activity of an unknown drug and, second, indicate its mechanism.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D008954:Models, Biological; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D013997:Time Factors
D000970:Antineoplastic Agents; D011073:Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine
Utilisation du taux des polyamines dans l'étude de l'activité antitumorale de substances naturelles; emploi de la régénération hepatique comme modele d'étude.