Quantitation of harringtonine and homoharringtonine in serum by high-performance liquid chromatography.
Harringtonine and homoharringtonine are naturally occurring alkaloids with demonstrated antineoplastic activity against certain types of leukemias in cell cultures, experimental animals, and initial clinical trials. Sample preparation consists of addition of the internal standard (one compound used as the internal standard for the other), solvent extraction with methylene chloride, washing with ammonium formate, and evaporation to dryness. The residue is dissolved in the mobile phase (40% methanol-60% 0.1 M ammonium formate) and an aliquot is chromatographed on a microC 18 reversed-phase column (flow-rate 1.5 ml/min). Peaks are detected with a spectrophotofluorimeter by monitoring the emission at 320 nm with excitation wavelength of 280 nm. Limit of detection is 10 ng/ml (20 nM) for both compounds; reproducible quantitation can be made to 30 ng/ml (60 nM).
D000470:Alkaloids / Q000097:blood*; D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006248:Harringtonines / Q000097:blood* / Q000302:isolation & purification; D000077863:Homoharringtonine; D051379:Mice; D051381:Rats; D013050:Spectrometry, Fluorescence
D000470:Alkaloids; D000970:Antineoplastic Agents; D006248:Harringtonines; D000077863:Homoharringtonine