Alkalinization of the incubation medium by rat and rabbit renal cortical slices.
The pH of bathing media containing acetate increased substantially during a 90-min incubation of renal cortex slices from rats and rabbits and did not achieve a steady state after 5 h. Alkalinization of the medium was markedly depressed by cold (0 degree C) and nearly abolished by incubation under 100% N2. Ammonia synthesis did not appear to be an important additional source of base. Incubation of tissue from either species in acetate-free medium reduced but did not abolish base production. Acetazolamide further reduced base production by rat kidney. It was concluded that a portion of the base added to the medium by rat renal tissue reflects the operation of a transport process that separates hydrogen and hydroxyl ions and may be the same one that normally secretes hydrogen ion into tubular fluid.
D000085:Acetates / Q000378:metabolism; D000086:Acetazolamide / Q000494:pharmacology; D000138:Acidosis / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D002021:Buffers; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000378:metabolism*; D008297:Male; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
D000085:Acetates; D002021:Buffers; D003470:Culture Media; D000641:Ammonia; D000086:Acetazolamide