[Horseradish peroxidase: reagent for stopping the catalytic conversion of the substrates H2O2 and 2,2'-azino-di(3-ethyl-benzathiazoline-sulphonic acid-(6)) (ABTS)].
Catalytic conversion of H2O2 and 2,2'-azino-di(3-ethyl-benzthiazoline-sulphonic acid-(6)) (ABTS) by peroxidase is stopped immediately and completely by the addition of a secondary alkyl sulphate (Teepol-610) or sodium dodecyl hydrogensulphate. The resulting colour intensity of the oxidized ABTS+ is thereby stabilized and can be determined photometrically within 60 min. These stop reagents have special practical importance for the enzyme-(peroxidase)-immunological determination by the ELISA technique; but the activity of the indicator enzyme must be measured by the two point method, owing to the heterogeneity of the test system and the slow reaction rate.
D052160:Benzothiazoles; D002384:Catalysis; D004797:Enzyme-Linked Immunosorbent Assay; D005233:Fatty Alcohols / Q000494:pharmacology*; D006735:Horseradish Peroxidase / Q000037:antagonists & inhibitors*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007202:Indicators and Reagents; D010544:Peroxidases / Q000037:antagonists & inhibitors*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D013451:Sulfonic Acids / Q000378:metabolism*
D052160:Benzothiazoles; D005233:Fatty Alcohols; D007202:Indicators and Reagents; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D012967:Sodium Dodecyl Sulfate; C026997:teepol 610; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D010544:Peroxidases
Peroxidase aus Meerrettich: Reagens zum Abstoppen der katalytischen Umsetzung der Substrate H2O2 und 2,2'-Azino-di(3-ethyl-benzthiazolinsulfonsäure-(6)) (ABTS).