[Blood serum proteins as possible indicators of burn intoxication].
Blood serum proteins from normal and burnt rats (15-20% of the body surface) were fractionated with ammonium sulfate and gel filtration on Sephadex G-200. Analysis of the composition was performed by disc electrophoresis in 7.5% polyacrylamide gel. Blood serum of burnt rats is characterized by alterations in the relative content of protein fractions as compared with normal rats' serum. Additional components appearing after burns are absent from normal serum and differ from burn skin toxin. According to the physiochemical analysis data, no component corresponding to burn toxin could be found either in the whole serum or in the fractionation products.
D000818:Animals; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000032:analysis*; D002056:Burns / Q000097:blood*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D051381:Rats; D011919:Rats, Inbred Strains; D014115:Toxemia / Q000097:blood*; D014118:Toxins, Biological / Q000097:blood
D001798:Blood Proteins; D014118:Toxins, Biological
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