[Lyophilization protection of hemoglobin by N-acetyltryptophan].
Measurements of methemoglobin content, different oxyphoric powers, oxygen affinity were made on freeze-dryed hemoglobin without protector and in presence of N-acetyl tryptophan and/or alpha-D glucose. These measurements revealed that, at low concentrations, N-acetyl tryptophan offers a better protection than glucose. Moreover, this acetylated amino-acid decreases the oxygen affinity of hemoglobin (Po(2)50 increased). The two protectors do not seem to compete, but present complementary effects.
D001692:Biological Transport; D001793:Blood Preservation; D003890:Desiccation; D005612:Freeze Drying / Q000379:methods; D005947:Glucose / Q000494:pharmacology; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D008706:Methemoglobin / Q000378:metabolism; D010108:Oxyhemoglobins / Q000032:analysis; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000494:pharmacology
D006454:Hemoglobins; D010108:Oxyhemoglobins; C006392:N-acetyltryptophan; D014364:Tryptophan; D008706:Methemoglobin; D005947:Glucose
Lyoprotection de l'hémoglobine par le N-acétyl tryptophane.