Characterisation of cells cultured from advanced atherosclerotic lesions of the rabbit.
The present study focused on cells cultured from late atherosclerotic lesions produced in rabbits by 6 months' cholesterol feeding followed by another 6 months' feeding with a normal rabbit chow. The growth rates of cells from both atherosclerotic and normal aortas were equal. The incorporation of [3H]proline into total protein and collagen as well as into collagen types I and III was also similar in cells from both origins. The cells from atherosclerotic rabbit aortas incorporated less [3H]glucosamine into sulphated glycosaminoglycans, but more into hyaluronic acid than cells from normal aortas. Cells from both origins were typical arterial smooth muscle cells as judged by electron microscopic examination. The results show that the enhanced metabolism of cells cultured from proliferative atherosclerotic lesions of the rabbit is no longer present in cells cultured from more advanced lesions.
D000818:Animals; D001011:Aorta / Q000166:cytology; D001161:Arteriosclerosis / Q000378:metabolism / Q000473:pathology*; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism; D011817:Rabbits
D006025:Glycosaminoglycans; D009124:Muscle Proteins; D006820:Hyaluronic Acid; D003094:Collagen