title: Does tissue ATP content limit active sodium transport across intestinal epithelia in vitro?
abstract: The ATP content of isolated epithelia of rabbit descending colon, incubated in oxygenated Ringer solution containing glucose, is increased by addition of 1 mM adenosine from 10.9 +/- 1.4 to 22.2 +/- 2.3 pmoles/mg, but the transport rate of the Na pump is not altered. It is therefore concluded that epithelial ATP synthesis is not rate-limiting for Na transport in this tissue under conditions of in vitro aerobic incubation.
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D004848:Epithelium / Q000032:analysis / Q000378:metabolism; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000032:analysis; D011817:Rabbits; D012964:Sodium / Q000378:metabolism*
chemical_list: D000255:Adenosine Triphosphate; D012964:Sodium; D000241:Adenosine