title: The biochemical effects of CPDA-2-drawn red blood cells of delayed refrigeration prior to component preparation.
abstract: The effects of an 8-hour hold at 22 degrees C prior to component preparation were evaluated in a split-bag study using nine units of blood preserved in citrate-phosphate-dextrose-adenine (CPDA-2). Each unit was divided in half, platelet-rich plasma removed at 0 or 8 hours, respectively, and the half units of red blood cells stored at 4 degrees C for 42 days. The only red blood cell metabolic differences seen in the bags held 8 hours (compared to those not held) were a 21 percent rise in adenosine triphosphate, which was not significant after 14 days of storage, and a 33 percent loss of 2,3-diphosphoglycerate which resulted in a loss curve similar to that seen with acid-citrate-dextrose blood. The logistic advantages seem to warrant an 8-hour holding period for red blood cells drawn in CPDA-2.
mesh_terms: D000225:Adenine / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000097:blood; D001786:Blood Glucose / Q000032:analysis; D001793:Blood Preservation / Q000379:methods*; D002469:Cell Separation; D002951:Citrates / Q000494:pharmacology*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004163:Diphosphoglyceric Acids / Q000097:blood; D004905:Erythrocyte Aging; D005947:Glucose / Q000494:pharmacology*; D006461:Hemolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010710:Phosphates / Q000494:pharmacology*
chemical_list: D001786:Blood Glucose; C034876:CPDA solutions; D002951:Citrates; D003451:Cryoprotective Agents; D004163:Diphosphoglyceric Acids; D010710:Phosphates; D000255:Adenosine Triphosphate; D005947:Glucose; D000225:Adenine