title: Tree shrew (Tupaia) herpesviruses.
abstract: Five Tupaia herpesviruses have been isolated until now: four in our laboratory which were termed THV-2, 3, 4, and 5, whereas THV-1 has been isolated by Melnick and his colleagues. THV-2 was isolated from tumour cell culture of a high-grade malignant lymphoma of a Tupaia, THV-3 was released from a cell culture of another Tupaia lymphoma, THV-4 from a spleen tissue culture of a moribund animal with finely granulated liver cirrhosis, and THV-5 from cultured spleen cells of an apparently healthy tree shrew. THV-1 to 5 were efficiently propagated, plaque-purified and cloned on Tupaia embryonic fibroblasts. The five isolates of Tupaia herpesviruses are easily distinguished from each other by restriction enzyme analysis of their genomes. THV-1 to 4 are highly pathogenic (lethality 100%) for juvenile Tupaias by intravenous inoculation. In contrast, only 25% lethality was found by intraperitoneal administration. THV-1 to 4 can persist as a latent infection in spleens of Tupaias and rabbits, which allows the recovery of infectious virus from cultured spleens of both animals. THV-2 and 3 induced hyperplasia of the thymus of rabbits which developed malignant thymoma in a few cases. The biological properties and genomic size and structure indicate that THV cannot be considered to belong to one of the three existing subfamilies of herpesviruses.
mesh_terms: D000818:Animals; D006224:Cricetinae; D004279:DNA, Viral / Q000032:analysis; D006564:Herpesviridae / Q000302:isolation & purification* / Q000472:pathogenicity / Q000648:ultrastructure; D051379:Mice; D009369:Neoplasms / Q000209:etiology / Q000662:veterinary; D011817:Rabbits; D051381:Rats; D014417:Tupaia / Q000382:microbiology*; D014418:Tupaiidae / Q000382:microbiology*
chemical_list: D004279:DNA, Viral