Effect of 5-bromodeoxyuridine on the synthesis of LDH-X in the postnatal testis of mouse.
The significance of normal DNA synthesis in the postnatal testis of mouse has been tested by using 5-bromodeoxyuridine (BUdR) on various days of development. Treatments with the drug were given for 48 h to the pups of different age groups starting immediately after birth up to day 8 post partum (PP). The appearance of the testis specific X-isozyme of lactate dehydrogenase (LDH-X) on day 17 PP has been used as a marker of spermatocyte differentiation. Autoradiographic studies on the testes of 4 to 5-day-old mice indicated that the highest percentage of prespermatogonia were labelled with 3H-BUdR. While a significant decline was noted in LDH-X synthesis on day 17 PP in the pups treated with BUdR on day 4-5 PP, other experimental animals subjected to similar treatments with BUdR on days 0-1, 2-3 and 6-7 PP showed no such decline. These results indicate that the prespermatogonia in day 4-5 PP testis are particularly susceptible to BUdR which blocks further differentiation. The possible reasons for this effect are discussed.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D013091:Spermatogenesis / Q000187:drug effects*; D013093:Spermatogonia / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*; D013737:Testis / Q000187:drug effects* / Q000201:enzymology; D013997:Time Factors
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D001973:Bromodeoxyuridine