Effect of different levels of vitamin B6 in the diet of rats on the content of pyridoxamine-5'-phosphate and pyridoxal-5'-phosphate in the liver.
The pyridoxal-5'-phosphate (PLP) and pyridoxamine-5'-phosphate (PMP) contents were determined in livers of rats fed diets containing different levels of vitamin B6 (0.3-22.9 ppm). Maximum growth was attained at vitamin B6 intake levels of greater than or equal to 24 micrograms/day. Maximum PLP and PMP levels in a crude liver homogenate were reached at intake levels of 75 and 9 micrograms/day respectively. In the 100 000 g supernatant fraction of liver the maximum PLP level was reached at intake levels of greater than or equal to 37 micrograms/day. The PLP/PMP-ratio of the crude liver homogenate increased from 0.6 up to values of appr. 1.5 at the higher intake levels. The PLP/PMP-ratio in the 100 000 g supernatant was more variable and did not show a consistent trend at varying vitamin B6 intakes.
D000818:Animals; D004032:Diet; D004305:Dose-Response Relationship, Drug; D006128:Growth / Q000187:drug effects; D008099:Liver / Q000378:metabolism*; D008297:Male; D011731:Pyridoxal Kinase / Q000378:metabolism; D011732:Pyridoxal Phosphate / Q000378:metabolism*; D011733:Pyridoxamine / Q000031:analogs & derivatives* / Q000378:metabolism; D011734:Pyridoxaminephosphate Oxidase / Q000378:metabolism; D011736:Pyridoxine / Q000008:administration & dosage*; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000378:metabolism
D011732:Pyridoxal Phosphate; D011733:Pyridoxamine; D011734:Pyridoxaminephosphate Oxidase; D011731:Pyridoxal Kinase; D011736:Pyridoxine; C010627:pyridoxamine phosphate