Decondensation of human sperm nuclei by glycosamineglycan-sulfate from sea urchin egg.
Decondensation of human and sea urchin spermatozoan nuclei has been induced by exposure of intact spermatozoa to glycosamineglycan-sulfate (GAGs) extracted from eggs of the sea urchin. Toxopneustes roseus. Swelling of human spermatozoan nuclei commenced about 60 min after the addition of GAGs and, depending on the extract concentration, the proportion of swollen nuclei reach 90% after 6 h of incubation when 20 mg of GAGs per ml was used. Swelling of sea urchin spermatozoan nuclei could be induced also with GAGs and/or with heparin. Swelling commenced about 10 min after the addition of GAGs/heparin reaching 90-92% after 60 min of incubation with 5 mg GAGs/500 USP heparin per ml. Preincubation of spermatozoa with 10 mg/ml of trypsin soybean inhibitor did not interfere with the swelling action of the GAGs.
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects* / Q000648:ultrastructure; D005260:Female; D006025:Glycosaminoglycans / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008297:Male; D010063:Ovum / Q000032:analysis*; D012617:Sea Urchins; D013094:Spermatozoa / Q000648:ultrastructure*
D006025:Glycosaminoglycans; C013786:A73025; D006493:Heparin