title: [Methodology of the colorimetric determination of alkylating cytostatics with 4-(4'-nitrobenzyl)pyridine (NBP) in plasma].
abstract: The sensitivity of the colorimetric determination of alkylating cytostatics with 4-(4'-nitrobenzyl)pyridine (NBP) has been increased by modifying procedures described in the literature. This increase in sensitivity was accomplished as follows: --Precipitation of protein with absolute ethanol at degree C; --reduction of the sample volume by evaporating the ethanol extract in a nitrogen flow; --control of the optimum pH value by means of a micro-glass electrode; --meticulously timed working under cooling in a dimmed room. The total alkylating activity and the part of endogenously activated cyclophosphamide were determined in four patients.
mesh_terms: D000477:Alkylating Agents / Q000097:blood*; D000970:Antineoplastic Agents / Q000097:blood*; D003124:Colorimetry / Q000379:methods; D004566:Electrodes; D006801:Humans; D007202:Indicators and Reagents; D011506:Proteins / Q000302:isolation & purification; D011725:Pyridines
chemical_list: D000477:Alkylating Agents; D000970:Antineoplastic Agents; D007202:Indicators and Reagents; D011506:Proteins; D011725:Pyridines; C008633:4-(4-nitrobenzyl)pyridine
vernacular_title: Zur Methodik der kolorimetrischen Bestimmung von alkylierenden Cytostatica mit 4-(4'-Nitrobenzyl)pyridin (NBP) aus Plasma.