title: Importance of maintaining species homology in thyroid hormone radioimmunoassays: modification of 'human' radioimmunoassay kits for use with rat samples.
abstract: Lack of species homology in radioimmunoassays can lead to serious errors. We have shown that a highly significant bias results from application of commercially available 'human' thyroxine and triiodothyronine assays to rat samples. This paper describes a simple modification whereby this source of error is overcome. Validation of the modified assays demonstrated their high degree of reliability.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D006801:Humans; D011817:Rabbits; D011863:Radioimmunoassay / Q000295:instrumentation; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013963:Thyroid Hormones / Q000032:analysis*; D013974:Thyroxine / Q000032:analysis; D014284:Triiodothyronine / Q000097:blood
chemical_list: D013963:Thyroid Hormones; D014284:Triiodothyronine; D013974:Thyroxine