[Thermoinactivation of bacterial luciferase].
The kinetics of thermal inactivation of bacterial luciferase was studied. It was found that the reaction substrates produce a weak protecting effect against the enzyme inactivation. EDTA, dithiothreitol and bovine serum albumin considerably stabilize the luciferase. The hypothetical mechanism of the enzyme inactivation involves oxidation of SH-groups and dissociation of the dimer into inactive monomers.
D007700:Kinetics; D008156:Luciferases / Q000037:antagonists & inhibitors*; D008433:Mathematics; D010776:Photobacterium / Q000201:enzymology*
D008156:Luciferases
Termoinaktivatsiia bakterial'noĭ liutsiferazy.