Ethylene production from alpha-oxo-gamma-methylthiobutyric acid is a sensitive measure of ligninolytic activity by Phanerochaete chrysosporium.
Production of hydroxyl radical in ligninolytic cultures was determined by measuring the alpha-oxo-gamma-methylthiobutyric acid-dependent production of ethylene gas. The results showed that the pattern of ethylene production was very similar to that of ligninolytic activity [[14C]lignin leads to 14CO2). Furthermore, nutritional parameters, which are known to affect ligninolytic activity, affected OH.-radical-dependent ethylene production in a similar fashion. The results indicate that assay for ethylene production from alpha-oxo-gamma-methylthiobutyric acid is a simple and sensitive measure of ligninolytic activity by Phanerochaete chrysosporium.
D001487:Basidiomycota / Q000378:metabolism*; D005030:Ethylenes / Q000096:biosynthesis*; D005609:Free Radicals; D008031:Lignin / Q000378:metabolism*; D008715:Methionine / Q000031:analogs & derivatives* / Q000378:metabolism; D008722:Methods
D005030:Ethylenes; D005609:Free Radicals; C014632:2-keto-4-methylthiobutyric acid; D008031:Lignin; C036216:ethylene; D008715:Methionine