Simultaneous determination of free 3-methoxy-4-hydroxymandelic acid and free 3-methoxy-4-hydroxyphenylethyleneglycol in plasma by liquid chromatography with electrochemical detection.
A new assay method is described for the simultaneous determination of free 3-methoxy-4-hydroxymandelic acid and 3-methoxy-4-hydroxyphenylethyleneglycol in plasma utilizing separation and purification by Bio-Gel P-10 followed by high-performance liquid chromatography with electrochemical detection. This technique is sensitive and reliable, and offers an inexpensive and practical alternative to gas chromatographic--mass fragmentographic methods for the monitoring of plasma levels of these catecholamine metabolites in the study of selective metabolic pathways of endogenous norepinephrine originating in the peripheral and the central nervous systems.
D000328:Adult; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods; D004563:Electrochemistry; D006018:Glycols / Q000097:blood*; D006801:Humans; D008734:Methoxyhydroxyphenylglycol / Q000097:blood* / Q000302:isolation & purification; D009420:Nervous System / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D014642:Vanilmandelic Acid / Q000097:blood* / Q000302:isolation & purification
D006018:Glycols; D008734:Methoxyhydroxyphenylglycol; D014642:Vanilmandelic Acid; D009638:Norepinephrine