Hematopoietic responses to lipopolysaccharide in C57BL/10Sn and C57BL/10ScN strain mice.
Inbred mouse strains C57BL/10Sn (Sn) and C57BL/10ScN (ScN) differ in response of their hematopoietic system to injection of lipopolysaccharide-W (LPS-W) in a manner similar to that observed for the LPS unresponsive C3H/HeJ and the paired responsive C3H/HeN strain mice. Responses of endogenous (E-CFU) stem cells as well as bone marrow and spleen-derived exogenous (CFU-s) stem cells, granulocyte-macrophage (GM-CFC) and macrophage (M-CFC) colony-forming cells were determined for Sn and ScN strain mice following an intraperitoneal injection of 10 micrograms LPS-W. Sn strain mice responded characteristically in terms of every parameter measured. Marrow-derived parameters reflected release of nucleated cells and early decrease of CFU-s, GM-CFC, and M-CFC followed by return toward control values. Peak splenic responses were observed within 4-5 days, whereas E-CFU increased significantly within 24 h after LPS-W. These responses were in marked contrast to those observed for the ScN strain mice, which were relatively unresponsive in terms of each parameter measured after LPS-W injection. These results show that the phenotypic expression of the defective LPS locus recently described in the ScN strain mice extends to those cells that control the response of the hematopoietic system to LPS, and is similar in every respect to those responses observed for the mutant LPS defective, C3H/HeJ strain mice.
D000818:Animals; D003114:Colony-Forming Units Assay; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D007928:Lethal Dose 50; D008050:Lipid A / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D013154:Spleen / Q000166:cytology
D008050:Lipid A; D008070:Lipopolysaccharides