Novel antigens of oral Actinomyces species prepared from a cell wall enzyme lysate.
Purified cell walls of Actinomyces viscosus and Actinomyces naeslundii were solubilized by the enzymatic action of Ml N-acetylmuramidase. Soluble cell wall carbohydrate antigens were purified by column chromatography. A purified antigen preparation (fraction C) from A. viscosus ATCC 19246 was found to contain deoxyhexoses (rhamnose and 6-deoxytalose), hexosse (galactose, glucose and mannose), and a peptidoglycan component. This preparation gave a single precipitin band against antiserum to homologous whole cells. Similar antigen preparations were purified from Ml enzyme lysate of A. viscosus SK4 and A. naeslundii ATCC 12104 cell walls.
D000190:Actinomyces / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D002473:Cell Wall / Q000276:immunology; D002845:Chromatography; D007106:Immune Sera / Q000276:immunology; D009113:Muramidase / Q000494:pharmacology; D011817:Rabbits; D012995:Solubility
D000942:Antigens, Bacterial; D007106:Immune Sera; D009113:Muramidase