Structural domains of ribosomal protein S8 and their relationship to ribosomal RNA binding.
Escherichia coli ribosomal protein S8 has been subjected to mild proteolytic digestion in order to search for structural domains within the protein [1]. A characteristic fragment produced in high yield after chymotrypsin treatment has been located with the protein sequence. Circular dichroism has shown this domain to be rich in alpha helix. However, the fragment loses its ability to bind to 16S rRNA as does a similar fragment produced by trypsin cleavage. The intact protein is required for rRNA binding and is highly protected against proteolytic digestion when bound to the RNA.
D000595:Amino Acid Sequence; D002918:Chymotrypsin / Q000378:metabolism; D008970:Molecular Weight; D011487:Protein Conformation; D012335:RNA, Ribosomal / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000032:analysis* / Q000378:metabolism
D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; C030254:ribosomal protein S8; D002918:Chymotrypsin