SCEs in lymphocytes of rats after exposure in vivo to cigarette smoke or to cyclophosphamide.
A lymphocyte culture system was used to study cytogenetic effects in rats after they had been exposed to genotoxic agents in vivo. The sensitivity of the test system was demonstrated by using cyclophosphamide. The SCE frequencies in lymphocytes of cyclophosphamide-treated rats (13.3 mg/kg; i.p.) was increased about 4-fold compared with those in lymphocytes from untreated rats. Female rats of the strain Wistar II were exposed to cigarette smoke from 30 cigarettes per day in an inhalation chamber. Lymphocyte cultures were performed after an acute exposure and after treatment for 4 weeks. After both treatments, the investigations of chromosomes with regard to SCEs did not reveal any effect.
D000818:Animals; D002478:Cells, Cultured; D003434:Crossing Over, Genetic / Q000187:drug effects*; D003520:Cyclophosphamide / Q000494:pharmacology*; D005260:Female; D008214:Lymphocytes / Q000187:drug effects*; D010947:Plants, Toxic; D051381:Rats; D011919:Rats, Inbred Strains; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D012906:Smoke; D014026:Nicotiana
D012906:Smoke; D003520:Cyclophosphamide