[Technique for isolation and culture of erythroblastic islands and separation of their central macrophage].
Erythroblastic islands (EI) were isolated using the adherence of their central macrophage to glass or plastic. The EI obtained were cultured and erythroblasts achieved a complete maturation within 72 h of incubation. When cultured in serum-free medium, the central macrophages were rapidly isolated, by detachment from their erythroblasts. Isolation and culture of central macrophages allow the study of their physiological characteristics and particularly the effect of their supernatant on erythropoïesis.
D000818:Animals; D001854:Bone Marrow Cells; D002469:Cell Separation; D002478:Cells, Cultured; D004912:Erythrocytes / Q000166:cytology*; D005260:Female; D008264:Macrophages / Q000166:cytology*; D008297:Male; D008722:Methods; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
Technique d'isolement et de culture des îlots érythroblastiques Séparation du macrophage central.