Correlation between the retention of cardiac glycosides in reversed-phase high-performance liquid chromatography with a diphenylsilyl stationary phase, the structure of their molecules and their biological activity.
The separation of mixtures of cardiac glycosides by reversed-phase high-performance liquid chromatography on silica gel with chemically grafted diphenylsilyl groups using water-ethanol as the eluent was carried out. It is shown that the configuration and conformation of the glycoside molecules, and the hydrophilic properties of their aglycones and glycones, influence the separation. The hydrophilic properties of the aglycones are more important than those of the glycones. The glycosides with more hydrophilic aglycones have higher biological activity. This is probably related to the easier transport of these glycosides to the receptor.
D000818:Animals; D002301:Cardiac Glycosides / Q000302:isolation & purification* / Q000378:metabolism; D002415:Cats; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D013329:Structure-Activity Relationship
D002301:Cardiac Glycosides