title: High-performance liquid chromatographic determination of ibuprofen and its major metabolites in biological fluids.
abstract: A sensitive and selective high-performance liquid-chromatographic assay for ibuprofen and its major metabolites in biological fluids is described. To ensure good chromatographic separation the drug and metabolites were run on a gradient elution system and detected with a variable wavelength detector set at 220 nm. A second, more rapid, isocratic system is also described for the detection of only ibuprofen.
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007052:Ibuprofen / Q000032:analysis* / Q000378:metabolism; D007700:Kinetics
chemical_list: D007052:Ibuprofen