A rapid semi-automated method for determining glycosylation in whole blood.
A rapid, semi-automated method for determining hexoses which are bound to components of whole blood; e.g., hemoglobin, is described. The method is an improvement on similar lengthy manual methods (1,2) in which 5-hydroxymethylfurfural is produced and measured. Heating of diluted blood samples at 15 lb./sq.in. above atmospheric pressure in a pressure cooker for 20 minutes is shown to give the same results as those obtained with other methods in which 2-5 hours heating at atmospheric pressure is specified (1,2,3). The trichloracetic acid precipitation step needed in the manual methods is avoided by continuous flow dialysis on a Technicom Auto Analyzer II system. Various experimental factors have been studied and comparisons made with a commonly-used ion-exchange resin column technique. Two different methods of calibration, one using pure 5-hydroxymethylfurfural standards, and the other using patients' samples with pre-determined, known values as standards, are presented.
D001322:Autoanalysis / Q000379:methods; D001786:Blood Glucose; D055598:Chemical Phenomena; D002621:Chemistry; D006442:Glycated Hemoglobin / Q000032:analysis; D006601:Hexoses / Q000097:blood*; D006358:Hot Temperature; D006801:Humans; D010070:Oxalates / Q000097:blood; D019815:Oxalic Acid; D013997:Time Factors
D001786:Blood Glucose; D006442:Glycated Hemoglobin; D006601:Hexoses; D010070:Oxalates; D019815:Oxalic Acid