Ornithine decarboxylase enzyme activity in human and hamster pancreatic tumor cell lines.
We measured ornithine decarboxylase (ODC) enzyme activity, protein and DNA levels in 4 pancreatic cancer cell lines, 2 derived from human tumors (COLO-357 and PANC-1) and 2 derived from hamster tumors (WDPaCa and PDPaCa). We measured the parameters in confluent stage conditions and in log-growth phase. We report that the enzyme levels in all cell lines are elevated with respect to normal pancreatic tissue. Half-life studies of ODC in the PDPaCa cell line indicate a 3-fold increase in half-life.
D000818:Animals; D002262:Carboxy-Lyases / Q000378:metabolism*; D002460:Cell Line / Q000032:analysis; D006224:Cricetinae; D004247:DNA; D006801:Humans; D008297:Male; D009374:Neoplasms, Experimental / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010179:Pancreas / Q000032:analysis; D010190:Pancreatic Neoplasms / Q000201:enzymology* / Q000378:metabolism; D011506:Proteins; D051381:Rats
D011506:Proteins; D004247:DNA; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase