Biliary excretion of chromium in the rat: a role of glutathione.
The relative amount of chromium excreted in rat bile after injection of Cr-III is much less than after injection of Cr-VI, about 0.1% and from 6-8% during 5 hours respectively, for corresponding dose levels. The liver to bile ratio was 50-100 for Cr-III injection, for Cr-VI the ratio was 2-3. With doses up to 18 mumol Cr/kg, only Cr-III was found in bile even after injection of Cr-VI. Glutathione depletion of the liver with cyclohexene oxide decreased chromium excretion in bile. Such treatment also decreased the reduction of Cr-VI to Cr-III in the liver cell as only Cr-VI was found in bile. A different distribution of Cr-III in the liver dependent on whether derived from Cr-VI or taken up by the liver as such must be assumed. Taking into account the usual low penetration of biological membranes by Cr-III, a possible active transport mechanism or a specific diffusable Cr-III compound must be postulated.
D000818:Animals; D001646:Bile / Q000378:metabolism*; D002712:Chlorides; D002840:Chromates / Q000378:metabolism; D002850:Chromatography, Gel; D002857:Chromium / Q000378:metabolism*; D017608:Chromium Compounds; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D007275:Injections, Intravenous; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D002712:Chlorides; D002840:Chromates; D017608:Chromium Compounds; D002857:Chromium; C016104:sodium bichromate; C017133:chromous chloride; D005978:Glutathione