Metabolism of N-hydroxyurethane by guinea pig liver preparations.
The present study demonstrated the metabolism of N-hydroxyurethane by cell-free preparations, i.e., 9000 X g supernatant, cytosol and microsomes, from guinea pig livers. Under anaerobic conditions, the metabolizing activities of these preparations were enhanced markedly by addition of both an NADPH- or NADH-generating system and FAD. When the 30-45% ammonium sulfate fraction from liver cytosol was combined with liver microsomes or milk xanthine oxidase, the metabolic reaction of N-hydroxyurethane proceeded to a greater extent. Thin-layer chromatographic examination showed that urethane was only a metabolite formed from N-hydroxyurethane by these preparations.
D000818:Animals; D002273:Carcinogens / Q000378:metabolism*; D002474:Cell-Free System; D005182:Flavin-Adenine Dinucleotide / Q000494:pharmacology; D006168:Guinea Pigs; D006877:Hydroxamic Acids / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D014520:Urethane / Q000031:analogs & derivatives*
D002273:Carcinogens; D006877:Hydroxamic Acids; D005182:Flavin-Adenine Dinucleotide; D014520:Urethane; C011286:N-hydroxyurethane