Action spectra for 8-methoxypsoralen and 3-carbethoxypsoralen in skin fibroblasts in vitro.
8-methoxypsoralen (8-MOP) and 3-Carbethoxypsoralen (3-CPs) are known photoreagents which have been employed in the treatment of psoriasis. Using skin fibroblasts grown in vitro we have determined the action spectra of both compounds in the long ultraviolet range. Narrow-band interference filters were applied to a Xenon lamp. Reduction in methyl-3H-thymidine (3H-TdR) uptake served as a parameter for photoinhibited cells. The results show a linear increate of 8-MOP-mediated photoinhibition with increasing wavelengths; 3-CPs showed comparatively weak inhibitory rates at the lower wavelength range (349-365 nm). Possibly this is due to the formation of 3-CPs photoproducts which are unreactive with DNA. Compared to 8-MOP, the monofunctional photoreagent 3-CPs is a weak photoinhibitor.
D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D011564:Furocoumarins / Q000494:pharmacology*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008730:Methoxsalen / Q000494:pharmacology*; D012867:Skin / Q000166:cytology; D013936:Thymidine / Q000378:metabolism; D014466:Ultraviolet Rays
D011564:Furocoumarins; D004247:DNA; C017104:3-carbethoxypsoralen; D008730:Methoxsalen; D013936:Thymidine