title: Intracellular nucleocapsid RNA of mumps virus.
abstract: Intracellular nucleocapsids, virtually free of host proteins and nucleic acids, were purified from cells acutely infected with several strains of mumps virus. VIral RNA obtained from the nucleocapsids was predominantly a single 50S species, with 28S RNA occurring as a secondary peak in the RNA profile. Nucleocapsids yielded approx. 9 micrograms 50S RNA per 10(9) infected cells. The 50S RNA was partially self-complementary, indicating that mumps viral nucleocapsids include full-length plus (+) sense antigenomes. Self-annealing and competition hybridization assays with 50S RNA suggest that 28S RNA includes duplexes consisting of full-length (+) and minus (-) sense 50S RNA. Properties of the viral RNA did not vary with the method of RNA isolation, time post-infection at which nucleocapsids were harvested, nor the strain of mumps virus.
mesh_terms: D000818:Animals; D002213:Capsid / Q000302:isolation & purification*; D002460:Cell Line; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D007668:Kidney; D008970:Molecular Weight; D009109:Mumps virus / Q000032:analysis*; D009698:Nucleoproteins / Q000302:isolation & purification*; D012330:RNA, Double-Stranded / Q000302:isolation & purification; D012367:RNA, Viral / Q000302:isolation & purification*; D012261:Ribonucleoproteins / Q000302:isolation & purification*; D014764:Viral Proteins / Q000302:isolation & purification*
chemical_list: D009698:Nucleoproteins; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012261:Ribonucleoproteins; D014764:Viral Proteins