Sensitivity of follicular melanoblasts in newborn mouse skin to tritiated thymidine: Evidence for a long term retention of label.
Injection of tritiated thymidine into newborn mice results in a progressive greying of hair that does not begin until after the first hair coat is grown. After a year the depigmentation is appreciable (about 60% of the hair are white). The effect cannot be simulated by external irradiation of newborn mice or by the administration of radioactive uridine or methionine. The effect can best be explained by a long-term retention of radioactivity in the DNA of melanocyte stem cells (melanoblasts) in spite of several rounds of cell division. This could be achieved by labelling the strands of DNA destined to act as templates throughout life by being selectively retained in the stem line as described in Cairns' hypothesis.
D000818:Animals; D000831:Animals, Newborn; D001973:Bromodeoxyuridine / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D005260:Female; D006197:Hair / Q000502:physiology; D008297:Male; D008544:Melanocytes / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D010860:Pigments, Biological / Q000378:metabolism; D012879:Skin Physiological Phenomena; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium
D010860:Pigments, Biological; D014316:Tritium; D001973:Bromodeoxyuridine; D013936:Thymidine