Lymphoma cell variants of decreased glucocorticoid sensitivity.
Glucocorticoid-sensitive S49.1 mouse lymphoma cells were mutagenized and cloned in soft agar containing 10 nM dexamethasone. A series of clones were grown and tested for growth inhibition by dexamethasone. While most clones were completely resistant to the steroid, some were sensitive but required significantly higher glucocorticoid concentrations for the same response than wild-type cells. Two of these low-sensitivity clones were used for binding studies; they showed significantly decreased levels of glucocorticoid receptors as compared to wild-type cells. The data support the view that the level of cellular steroid hormone receptors quantitatively controls hormone responsiveness in closely related cells.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007621:Karyotyping; D008223:Lymphoma / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D009154:Mutation; D009374:Neoplasms, Experimental / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D003907:Dexamethasone