title: Cellular DNA content in different species of Xenopus.
abstract: 1. Biochemical methods were applied to determine the DNA content per cell in ten species and subspecies of Xenopus. 2. Cell density and DNA concentration were measured in a suspension of blood cells. 3. DNA was determined by the diphenylamine method and values were compared with those obtained by the ultraviolet absorption method and by cytofluorometry. 4. A good degree of reproducibility was demonstrated by a simple biochemical method which yielded values compatible with cytofluorometry. 5. Results suggested that the DNA of Xenopus muelleri, and perhaps that of other species with a large genome size, may be specially protected from chemical degradation.
mesh_terms: D000818:Animals; D002452:Cell Count; D004247:DNA / Q000032:analysis*; D005189:False Positive Reactions; D006358:Hot Temperature; D006868:Hydrolysis; D008722:Methods; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D014981:Xenopus / Q000235:genetics*
chemical_list: D004247:DNA