An improved method for the preparation of highly purified satietin samples from human serum.
Satietin, a highly selective anorectic substance, was prepared in highly purified form. A new isolation procedure is described for the preparation of human satietin. Serum proteins were removed by ultrafiltration and precipitation with trichloroacetic acid and the satietin active substances were then separated by gelchromatographic and electrophoretic techniques. The highly purified materials are proved to show carbohydrate properties. The carbohydrate and protein content of samples were determined by amino acid and sugar analysis after acid hydrolysis of samples. According to a specific bioassay these substances were found to contain 100 units/mg activity, and concluded to be glycoproteins having molecular weight around of 50,000.
D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer / Q000379:methods; D004586:Electrophoresis; D006020:Glycopeptides / Q000097:blood / Q000302:isolation & purification*; D006801:Humans; D014462:Ultrafiltration
D000596:Amino Acids; D006020:Glycopeptides; C035102:satietin