[Spectrophotometric evaluation of N-acetyl-beta-glucosaminidase in urine].
A spectrophotometric method for the assay of N-Acetyl-beta-Glucosaminidase activity in human undiluted urines is described. The application of this method is recommended for its sensitivity (2,6 X 10(-4)M) and its rapid performance, because it represents a good alternative to current methods and essentially to the fluorimetric technique with which it has a significant statistical correlation. Estimates of normal individuals aged between 1-70 years are reported.
D000117:Acetylglucosamine / Q000031:analogs & derivatives*; D000118:Acetylglucosaminidase / Q000652:urine*; D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D005260:Female; D005944:Glucosamine / Q000031:analogs & derivatives; D006596:Hexosaminidases / Q000652:urine*; D006801:Humans; D006923:Hymecromone / Q000031:analogs & derivatives; D007223:Infant; D007674:Kidney Diseases / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry / Q000379:methods*; D013379:Substrate Specificity
C025158:4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside; D006923:Hymecromone; D006596:Hexosaminidases; D000118:Acetylglucosaminidase; D005944:Glucosamine; D000117:Acetylglucosamine
Valutazione spettrofotometrica della N-acetil-beta-glucosaminidasi nelle urine.