Antibody response to rubella virus proteins in different physical forms.
The immunogenicity of different antigens, containing rubella virus hemagglutinating (HA) membrane protein, was studied using live virus, beta-propiolactone-inactivated virus, detergent and lipid-free octamers and virosomes. Whole virus particles, live or inactivated, induced hemagglutination inhibition (HAI) antibodies in rabbits after one subcutaneous injection of 0.16 micrograms of HA protein. Hemagglutinin rosettes or virosomes failed to induce antibodies even at a dose of 120 micrograms. Apparently, the extraction of viral membrane hemagglutinin, for the preparation of a rubella subunit vaccine, led to destruction of the antigenicity responsible for the induction of hemagglutination inhibiting antibodies. These results are discussed in the light of earlier studies on the preparation of a rubella subunit vaccine.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D005620:Freund's Adjuvant; D006385:Hemagglutination Inhibition Tests; D006389:Hemagglutinins, Viral / Q000276:immunology*; D008081:Liposomes; D011817:Rabbits; D012412:Rubella virus / Q000254:growth & development / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology; D015678:Viral Structural Proteins
D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D008081:Liposomes; D014764:Viral Proteins; D015678:Viral Structural Proteins; D005620:Freund's Adjuvant