[Crabtree effect caused by ketoses in isolated rat hepatocytes].
Oxygen uptake and glycolytic activity were studied in hepatocytes isolated from fed rats. The addition of fructose or tagatose resulted in a 38% and 31% inhibition of cellular respiration respectively. The addition of 10 mM D-glyceraldehyde caused a slight Crabtree effect. Glucose, L-sorbose, or glycerol failed to modify oxygen consumption. Only incubation in the presence of fructose showed a high aerobic glycolysis measured by lactate production.
D000332:Aerobiosis / Q000187:drug effects; D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects; D005632:Fructose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D005985:Glyceraldehyde / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D006019:Glycolysis / Q000187:drug effects*; D006601:Hexoses / Q000494:pharmacology; D007661:Ketoses / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects*; D051381:Rats; D013013:Sorbose / Q000494:pharmacology
D006601:Hexoses; D007661:Ketoses; D005632:Fructose; D005985:Glyceraldehyde; D005947:Glucose; D013013:Sorbose; D005990:Glycerol; C030192:tagatose
Efecto Crabtree causado por cetosas en hepatocitos aislados de rata.