Enhancement of Lewis lung carcinoma growth by sera or spleen cells from tumour-bearing mice.
Lewis lung carcinoma(3LL) cells grafted in syngeneic adult C57BL/6 mice produced local tumours associated with lung metastasis in 78% of recipients. Adult thymectomized, lethally irradiated, bone marrow cell-reconstituted animals (B mice) were more resistant since this tumour grew in only 46% of recipients and especially the weight of lung metastasis was almost 8 times less than in normal animals. Sera from tumour-bearing mice transferred into B mice enhanced tumour incidence and weight of metastasis to the level observed in normal mice. In vitro cultured 3LL cells displayed an intense mitogenic activity as measured by 3H-thymidine incorporation. Addition into these cultures of serum from tumour-bearing animals did not alter this activity. Addition of normal spleen cells in a ratio 40/1 reduced this mitogenic activity to a half or a third. Spleen cells from tumour-bearing mice, either normal or serum-enhanced B mice, mixed in vitro with 3LL cells, produced a 4-fold increase of mitogenic activity of the latter. These results indicate that the lymphoid system may contribute to the growth and spreading of 3LL tumours.
D000818:Animals; D001803:Blood Transfusion; D002471:Cell Transformation, Neoplastic / Q000276:immunology*; D006082:Graft Enhancement, Immunologic; D008175:Lung Neoplasms / Q000276:immunology* / Q000556:secondary; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000494:pharmacology; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology; D013154:Spleen / Q000276:immunology
D008934:Mitogens