[Detection of 2 cell subpopulations each differing in incorporating low-density lipoproteins in an atherosclerotic plaque from the human aorta].
Cells were isolated by enzyme dispersion of plaques (N-cells) and unaffected areas (A-cells) of human aorta. The capacity of both types of cells to uptake LDL was compared in 7-day primary cultures. Also, these populations were analyzed to discover subpopulations with different types of LDL uptake. RITC-labeled LDL was used for the purpose. A fluorescent-activated cell sorter (FACS-II, Becton and Dickinson, USA) was used to measure the RITC-LDL content and to sort out cell subpopulations. It was shown that the A-cell population consists of two subpopulations with a different type of RITC-LDL uptake. Cells overloaded with lipid inclusions uptake RITC-LDL nonspecifically, while cells without lipid inclusions mostly uptake RITC-LDL through specific receptors.
D000328:Adult; D001011:Aorta / Q000378:metabolism / Q000473:pathology; D001018:Aortic Diseases / Q000378:metabolism / Q000473:pathology*; D001161:Arteriosclerosis / Q000378:metabolism / Q000473:pathology*; D002478:Cells, Cultured; D005434:Flow Cytometry; D005453:Fluorescence; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
D008077:Lipoproteins, LDL
Vyiavlenie v ateroskleroticheskoĭ bliashke aorty cheloveka dvukh subpopuliatsiĭ kletok, po-raznomu vkliuchaiushchikh lipoproteidy nizkoĭ plotnosti.