The effects of acrylamide on beta-glucuronidase and acid phosphatase activities in rat sciatic nerve above and below a ligature.
The enzymes beta-glucuronidase and acid phosphatase have been assayed by histochemical and by fluorimetric methods above and below a tightly tied ligature on the rat sciatic nerve over the subsequent 10 days. These findings have been compared with similarly treated animals also given four daily doses of acrylamide (50 mg/kg). The following have been found: 1 during this time, acrylamide at this dose causes slight increases in beta-glucuronidase in untied sciatic nerves, detectable both histochemically and fluorimetrically; 2 below the ligature both enzyme activities were greatly increased and this was slightly reduced in the acrylamide-dosed animals; 3 there was a mild rise in beta-glucuronidase activity in the 1.0 cm above the ligature in undosed animals demonstrable both histochemically and fluorimetrically; 4 that in the acrylamide-dosed animals there was a marked rise in beta-glucuronidase activity both 1.0 cm and 2.0 cm above the ligature which was intensified at 7 days and at 10 days after dosing. This was demonstrated both histochemically and fluorimetrically. These results are discussed in the light of the known sheath cell and axonal responses to acrylamide intoxication.
D000135:Acid Phosphatase / Q000378:metabolism*; D000178:Acrylamides / Q000494:pharmacology*; D000818:Animals; D005966:Glucuronidase / Q000378:metabolism*; D008026:Ligation; D008297:Male; D008722:Methods; D051381:Rats / Q000502:physiology*; D012584:Sciatic Nerve / Q000201:enzymology*; D014855:Wallerian Degeneration
D000178:Acrylamides; D000135:Acid Phosphatase; D005966:Glucuronidase