Setaria cervi: enzymes in microfilariae and in vitro action of antifilarials.
Microfilariae of bovine filarial parasite Setaria cervi are equipped with the enzymes of glycolysis, pentose phosphate and PEP-succinate pathways and thus resemble the adult form in its metabolic pattern. Malate dehydrogenase was the most active enzyme in microfilariae followed by lactic dehydrogenase and fumarase, while phosphoglucoisomerase, PEP-carboxykinase and FDP-aldolase were comparatively less active. The very low ratio of PK/PEPCK in S. cervi microfilariae indicates active fixation of CO2 into PEP to produce oxalacetate. Centperazine and diethylcarbamazine significantly inhibited PEP-carboxykinase, fumarate reductase and succinic dehydrogenase, suggesting that these antifilarials probably exert microfilaricidal action by blocking the PEP-succinate pathway.
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D004049:Diethylcarbamazine / Q000494:pharmacology; D005369:Filaricides / Q000494:pharmacology*; D005370:Filarioidea / Q000187:drug effects / Q000201:enzymology*; D005941:Glucokinase / Q000378:metabolism; D006019:Glycolysis; D008842:Microfilariae / Q000201:enzymology; D010088:Oxidoreductases / Q000378:metabolism; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010428:Pentosephosphates / Q000378:metabolism*; D010728:Phosphoenolpyruvate / Q000378:metabolism*; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism; D012719:Setariasis / Q000469:parasitology; D013385:Succinate Dehydrogenase / Q000378:metabolism
D000871:Anthelmintics; D005369:Filaricides; D010428:Pentosephosphates; D010728:Phosphoenolpyruvate; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C020155:fumarate reductase (NADH); D013385:Succinate Dehydrogenase; D005941:Glucokinase; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D004049:Diethylcarbamazine