Determination of clofilium, a new antifibrillatory agent, in plasma by gas chromatography--mass spectrometry.
A sensitive and selective method for the assay of the new quaternary amine antifibrillatory agent clofilium is described. Plasma samples were extracted with dichloromethane (98.5 +/- 0.2% recovery) and analyzed by gas chromatography--mass spectrometry operating in the electron-impact mode. The method involves a Hofmann elimination of an N-alkyl radical from clofilium and the internal standard in the presence of a strong nucleophile in the injector of the gas chromatograph. The resulting tertiary amines are chromatographed and detected by selective ion monitoring. The ratio of the clofilium base peak (m/z 224) to the internal standard peak (m/z 210) was linear relative to the plasma clofilium concentration over the range of 25-1000 ng/ml plasma.
D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000097:blood*; D004285:Dogs; D008401:Gas Chromatography-Mass Spectrometry; D000644:Quaternary Ammonium Compounds / Q000097:blood*; D013997:Time Factors
D000889:Anti-Arrhythmia Agents; D000644:Quaternary Ammonium Compounds; C022799:clofilium