Solid-phase radioimmunoassay for detection of plague antigen in animal tissue.
A rapid and sensitive radioimmunoassay for the detection of soluble Yersinia pestis fraction I antigen using antibody-coated beads and radiolabeled immunoglobulin was applied to spleen-liver extracts, rendered noninfectious by either treatment or filtration, from rodents dead of plague. The procedure was capable of detecting nanogram amounts of soluble plague antigen.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D003429:Cross Reactions; D051379:Mice; D011863:Radioimmunoassay; D015010:Yersinia pestis / Q000276:immunology*
D000942:Antigens, Bacterial