Effects of serum concentration on some growth characteristics of cultured murine parietal yolk sac carcinoma cells.
Murine parietal yolk sac carcinoma cells were examined by scanning and transmission electron microscopy to determine the ultrastructural changes resulting from growth, in vitro, in media containing different serum concentrations. Cells grown in medium supplemented with 10% fetal bovine serum (FBS) formed spherical bodies, were gradually oval with numerous surface microvilli, well-organized microtubules, abundant free polysomes and a well-developed Golgi apparatus. By contrast, cells grown in 1% FBS failed to form multicellular spheres, were generally flattened over the growth surface and lacked the surface and intracellular features demonstrated when cells were grown in 10% serum. These differences could explain the alterations in the glycosylation of secreted glycoprotein associated with culture in the presence of low serum.
D000818:Animals; D001769:Blood; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis / Q000648:ultrastructure; D003470:Culture Media; D004407:Dysgerminoma / Q000648:ultrastructure*; D006025:Glycosaminoglycans / Q000032:analysis; D007365:Intercellular Junctions / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008870:Microtubules / Q000648:ultrastructure; D008871:Microvilli / Q000648:ultrastructure; D009940:Organoids / Q000648:ultrastructure
D003470:Culture Media; D006025:Glycosaminoglycans