title: Rabbit fibrin: characterization of the separated chains.
abstract: In preparation for studies of the time course of production in vivo of the constituent chains of rabbit fibrin, we characterized the structural features of the fibrin molecule. Fibrin was isolated from plasma and reduced and alkylated. The alpha, beta, and gamma chains were separated by CM-cellulose chromatography and their molecular weights and amino acid compositions were determined. The gamma chain was sequenced 36 steps with 32 positive identifications and the beta chain, 12 steps with 12 identifications. No major differences between the sequences of these chains and those of man, chicken, and dog were noted.
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D005337:Fibrin / Q000032:analysis*; D008970:Molecular Weight; D011817:Rabbits / Q000097:blood*; D013045:Species Specificity
chemical_list: D000596:Amino Acids; D005337:Fibrin