A simple enzymic method for the synthesis of [32P]phosphoenolpyruvate.
A rapid and simple enzymic method is described for the synthesis of [(32)P]phosphoenolpyruvate from [(32)P]P(i), with a reproducible yield of 74%. The final product was shown to be a good substrate for pyruvate kinase (EC 2.7.1.40).
D002855:Chromatography, Thin Layer; D006160:Guanosine Triphosphate / Q000378:metabolism; D010728:Phosphoenolpyruvate / Q000138:chemical synthesis*; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D011770:Pyruvate Kinase / Q000378:metabolism; D011849:Radioactive Tracers
D010761:Phosphorus Radioisotopes; D011849:Radioactive Tracers; D010728:Phosphoenolpyruvate; D006160:Guanosine Triphosphate; D011770:Pyruvate Kinase; D010729:Phosphoenolpyruvate Carboxykinase (GTP)