[The bulk of tRNA in the extracts of animal cells is not complexed with proteins].
The bulk of tRNA from cytoplasmic cell extracts of rat liver sedimented in the postribosomal zone appears to be in a free state and is not complexed with proteins. Evidence for this assumption may be derived from the following experimental findings: i) the tRNA of the extract is not adsorbed on nitrocellulose filters under conditions of ribonucleoprotein sorption; ii) after formaldehyde fixation and contrifugation to equilibrium in CsCl or Cs2SO4 density gradients the tRNA of the extract is distributed as free tRNA; iii) the tRNA of the extract does not differ considerably from the deproteinated tRNA in sedimentation velocity.
D000818:Animals; D002499:Centrifugation, Density Gradient; D008099:Liver / Q000032:analysis*; D008297:Male; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification*; D012343:RNA, Transfer / Q000302:isolation & purification*; D051381:Rats; D012261:Ribonucleoproteins / Q000032:analysis
D011506:Proteins; D012261:Ribonucleoproteins; D012343:RNA, Transfer
Osnovnaia massa tRNK v ékstraktakh zhivotnykh kletok ne kompleksirovana s belkami.