Difference in plasma membrane structure between two sublines of Ehrlich-Lettrè ascites tumor cells.
The plasma membranes of the glycogen-free and the glycogen-containing subline of Ehrlich-Lettrè ascites cells were purified and compared with respect to their enzyme activity, chemical, lipid and protein composition, and membrane fluidity. Both membrane fractions differed in a number of parameters which are discussed as differences in the expression of malignant transformation of the two sublines. 1. The 5'-nucleotidase activity was 3-5 times higher and the sialic acid content 3-times lower in the glycogen-containing than in the glycogen-free subline. 2. Differences were also observed with respect to the phospholipid composition, that is in the relative proportions of mainly phosphatidylcholine, -inositol and -serine. 3. The fatty acid spectrum of the two sublines differed in the C-18 series and in the percentage of polyunsaturated acids, which was about 6% lower in the glycogen-containing line. 4. Measurements of fluorescence polarization (P) using 1,6-diphenyl-1,3,5-hextriene as probe generally gave higher P values, indicating a decreased membrane fluidity for the plasma membranes of the glycogen-containing subline both below and above the transition temperature at 33 degrees C. 5. Polyacrylamide gel electrophoresis revealed different protein patterns mainly in the molecular weight range of around 90 000 and in the range between 31 000 and 14 000.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000648:ultrastructure*; D002458:Cell Fractionation; D002462:Cell Membrane / Q000648:ultrastructure*; D002499:Centrifugation, Density Gradient; D002784:Cholesterol / Q000032:analysis; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis; D006003:Glycogen / Q000032:analysis; D008297:Male; D008563:Membrane Lipids / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis*; D051379:Mice; D008815:Mice, Inbred Strains; D010743:Phospholipids / Q000032:analysis; D013050:Spectrometry, Fluorescence
D005227:Fatty Acids; D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids; D006003:Glycogen; D002784:Cholesterol