Multiple transcripts of T-DNA detected in nopaline crown gall tumors.
Crown gall plant tumors contain neoplastic cells transformed by incorporation of a foreign DNA element, T-DNA, derived from a large tumor-inducing plasmid in the inciting Agrobacterium strain. T-DNA is covalently joined to the nuclear DNA of the tumor cell, and RNA transcripts from T-DNA are present in polyadenylated form on polysomes. This paper presents a detailed analysis of those parts of T-DNA transcribed in a nopaline-type tobacco teratoma, BT37, whose T-DNA has been mapped and cloned. Northern blots of polyA+ RNA were probed with 21 different nick-translated T-DNA fragments, and at least 13 well-defined transcripts were visualized.
D001120:Arginine / Q000031:analogs & derivatives / Q000235:genetics; D002478:Cells, Cultured; D004269:DNA, Bacterial / Q000235:genetics*; D010947:Plants, Toxic; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D012231:Rhizobium / Q000235:genetics*; D014026:Nicotiana; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D012333:RNA, Messenger; C008666:nopaline; D001120:Arginine