The effects of succinylacetone (4,6-dioxoheptanoic acid) on delta-aminolevulinate synthase activity and the content of heme in monolayers of chick embryo liver cells.
Succinylacetone was shown to inhibit aminolevulinate dehydratase (5-aminolevulinate hydro-lyase (adding 5-aminolevulinate and cyclizing), EC 4.2.1.24) to reduce cellular heme and porphyrins and to induce delta-aminolevulinate synthase (succinyl-CoA:glycine C-succinyltransferase (decarboxylating), EC 2.3.1.37) in monolayers of chick embryo liver cells. Marked synergistic effects on delta-aminolevulinate synthase activity were obtained by combining succinylacetone with levulinate and porphyrogenic drugs. The time course of delta-aminolevulinate synthase activity showed a delayed synergistic response.
D000624:5-Aminolevulinate Synthetase / Q000096:biosynthesis*; D000502:Allylisopropylacetamide / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004357:Drug Synergism; D004790:Enzyme Induction / Q000187:drug effects; D006418:Heme / Q000378:metabolism*; D006537:Heptanoates / Q000494:pharmacology*; D006538:Heptanoic Acids / Q000494:pharmacology*; D007982:Levulinic Acids / Q000494:pharmacology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D000623:Porphobilinogen Synthase / Q000037:antagonists & inhibitors
D006537:Heptanoates; D006538:Heptanoic Acids; D007982:Levulinic Acids; D000502:Allylisopropylacetamide; D006418:Heme; C020804:succinylacetone; D000624:5-Aminolevulinate Synthetase; D000623:Porphobilinogen Synthase