Studies on protein binding of antibiotics. IV. Effect of the binding of drug to 100,000 X g supernatant fluid of rabbit liver homogenates on urinary excretion.
The extent of the binding of beta-lactam antibiotics to 100,000 X g supernatant fluid of rabbit liver homogenates was investigated. Urinary excretion tended to decrease as the extent of the binding to 100,000 X g supernatant fluid increased. Predicting urinary excretion was achieved using the binding to 100,000 X g supernatant fluid and other physicochemical factors. A good coincidence resulted from multiple regression analysis. [14C]Benzylpenicillin binding proteins in rabbit liver were investigated and revealed that the affinity of cefoperazone was much stronger than that of cefazolin.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000652:urine*; D001798:Blood Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D007769:Lactams; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D011485:Protein Binding; D011817:Rabbits; D012044:Regression Analysis
D000900:Anti-Bacterial Agents; D001798:Blood Proteins; D002352:Carrier Proteins; D007769:Lactams