In vitro survival of chick embryo spinal neurons capable of forming synapses on skeletal muscle cells.
Survival of chick embryo spinal neurons capable of forming synapses on skeletal muscle cells (junction-forming neurons) was examined electrophysiologically in culture. Tissue extracts (skeletal muscle, heart, liver, brain), serum-free muscle-conditioned medium, horse serum (HS), chick embryo extract (CEE) showed survival-promoting activity. The highest activity was observed in a mixture of HS and CEE. This mixture did not promote the survival of all of the spinal cord cells, but selectively supported the survival of junction-forming neurons.
D000818:Animals; D001921:Brain / Q000502:physiology; D002470:Cell Survival; D002642:Chick Embryo; D003470:Culture Media; D006321:Heart / Q000502:physiology; D008099:Liver / Q000502:physiology; D009132:Muscles / Q000294:innervation* / Q000502:physiology; D009469:Neuromuscular Junction / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009924:Organ Culture Techniques; D013116:Spinal Cord / Q000502:physiology*; D013569:Synapses / Q000502:physiology*; D014020:Tissue Extracts / Q000494:pharmacology
D003470:Culture Media; D014020:Tissue Extracts