A method for hydrolyzing and determining the amino acid compositions of picomole quantities of proteins in less than 3 hours.
A method is described for quantitatively hydrolyzing proteins in 45 min and for analyzing the hydrolysates by high-performance liquid chromatography in an additional 52 min. The alpha-amino acids were detected by the fluorescence of their omicron-phthaldialdehyde derivatives. Ten picomoles of each of the commonly occurring alpha-amino acids could be reliably determined. The method described yielded OPA-ethanethiolamino acid derivatives that were stable for 1 1/2 h and the HPLC method produced a better separation than previously published methods.
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D023181:Antimicrobial Cationic Peptides; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006868:Hydrolysis; D008832:Microchemistry; D009113:Muramidase; D010940:Plant Proteins / Q000032:analysis; D010944:Plants; D011506:Proteins; D014908:Triticum
D000596:Amino Acids; D023181:Antimicrobial Cationic Peptides; D010940:Plant Proteins; D011506:Proteins; C016085:purothionin; D009113:Muramidase