The effect of exogenous DNA insertion at a chromosomal region containing rDNA.
We have previously shown that exogenous DNA may be incorporated into host cell chromosomes following DNA-mediated gene transfer (transformation). Discrete chromosomal changes often accompany the integration event. In this study, the effect of integration of DNA that codes for human growth hormone into a region containing ribosomal DNA in rat chromosomes was investigated. The results show that insertion of exogenous DNA results in the inactivation of the ribosomal DNA and a partial deletion of ribosomal DNA sequences.
D000818:Animals; D002460:Cell Line; D004247:DNA; D004275:DNA, Ribosomal; D004276:DNA, Satellite / Q000235:genetics; D013006:Growth Hormone / Q000235:genetics; D006801:Humans; D009693:Nucleic Acid Hybridization; D009697:Nucleolus Organizer Region; D051381:Rats; D013937:Thymidine Kinase / Q000235:genetics; D014170:Transformation, Genetic
D004275:DNA, Ribosomal; D004276:DNA, Satellite; D013006:Growth Hormone; D004247:DNA; D013937:Thymidine Kinase