The maintenance of cytochrome P-450 in liver cell culture: recent studies on P-450 mediated mechanisms of toxicity.
Rat hepatocytes have been cultured under conditions that result in low and high cytochrome P-450 concentrations and then used as a "screening test" to determine the involvement of cytochrome P-450 in toxicity. The results show that a limitation to the use of cultured hepatocytes in toxicity tests is the lack of extrahepatic release mechanisms of toxic compounds.
D000818:Animals; D002251:Carbon Tetrachloride / Q000494:pharmacology; D002478:Cells, Cultured / Q000201:enzymology*; D003470:Culture Media; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014118:Toxins, Biological / Q000494:pharmacology
D003470:Culture Media; D011506:Proteins; D014118:Toxins, Biological; D003577:Cytochrome P-450 Enzyme System; D002251:Carbon Tetrachloride