title: Calcium inactivation in skeletal muscle fibres of the stick insect, Carausius morosus.
abstract: 1. Inactivation of Ca currents in skeletal muscle fibres of the stick insect, Carausius morosus, was studied using a three-electrode voltage-clamp method. 2. The extent of inactivation showed a voltage-dependence similar to that of the Ca current, inactivation being absent in the absence of a Ca current, maximal at potentials where Ca currents are largest, and reduced at potentials close to ECa. 3. Ca currents inactivated along a double exponential time course, both when measured from the decline of Ca current during a single pulse and when measured using a two pulse protocol. In 20 mM-Ca-Ringer the fast time constant of inactivation had a mean value of 27 msec and that of the slow time constant was 134 msec, at O mV and 5 degrees C. 4. The rate of inactivation was slowed, and its extent reduced, in low [Ca]o, where Ca currents are smaller. Similarly, inactivation was faster and more complete in high-Ca-Ringer. 5. The rate of recovery from inactivation also followed a double exponential time course, with time constants of 638 msec and 4 sec following a 500 msec inactivating pulse in 20 mM-Ca-Ringer at 5 degrees C. Recovery appeared to be related to the amount of Ca entry during the inactivating pulse, being slower in high [Ca]o and following longer inactivating pulses. 6. Inactivation was slowed and reduced in extent when Ba2+ or Sr2+ carried current. Inactivation in Ba solutions may be due to depletion of Ba2+ from the lumen of the transverse tubules. 7. Ba2+ does not compete with Ca2+ for the inactivation mechanism. 8. It is concluded that inactivation of Ca currents in stick insect muscle fibres is primarily mediated by Ca2+ entry.
mesh_terms: D000818:Animals; D001464:Barium / Q000494:pharmacology; D002118:Calcium / Q000502:physiology*; D004553:Electric Conductivity; D004594:Electrophysiology; D007313:Insecta; D008564:Membrane Potentials; D009132:Muscles / Q000187:drug effects / Q000502:physiology*; D013324:Strontium / Q000494:pharmacology; D013997:Time Factors
chemical_list: D001464:Barium; D002118:Calcium; D013324:Strontium