The prenatal determination of fetal sex: amniotic fluid testosterone as a preliminary screening test.
Amniotic fluid testosterone levels were measured by radioimmunoassay without chromatography on 101 specimens obtained at amniocenteses between 15 and 19 wk gestation. For the male fetus, the amniotic fluid testosterone level of 553 +/- 23 pmol/l (mean +/- SE) was significantly higher (P less than 0.0005) than the concentration found for the female fetus (206 +/- 9 pmol/l). There was an overlap of the ranges 74-1120 pmol/l for the male and 122-399 pmol/l for the female fetuses. Amniotic fluid testosterone levels above 400 pmol/l were observed in 84% of the male and in none of the female fetuses. The method allowed determination of testosterone levels within 8 h. It is concluded that amniotic fluid testosterone measured by radioimmunoassay without chromatography is a rapid and effective preliminary screening test for the prenatal diagnosis of fetal sex.
D000649:Amniocentesis; D000653:Amniotic Fluid / Q000378:metabolism*; D003429:Cross Reactions; D005260:Female; D006801:Humans; D008297:Male; D011247:Pregnancy; D011863:Radioimmunoassay; D012732:Sex Determination Analysis; D013739:Testosterone / Q000378:metabolism*
D013739:Testosterone