Studies on antitumor effect of corynebacterium parvum and pustulan on Lewis lung carcinoma in mice.
Antitumor effect of two biological adjuvants and their supported mechanism was studied in mice inoculated s.c. with Lewis lung carcinoma (LL) cells. The studies included in vivo tests: median survival time (MST) evaluation, tumor weight inhibition (TWI) and splenohepatomegaly measurements. The nonspecific in vitro cytostatic effect of peritoneal adherent cells stimulated with adjuvants was measured by inhibition of 3H thymidine incorporation into tumor cells. In in vivo studies the reproducible 20-30% of TWI was achieved in groups treated with both drugs, while no significant increase in MST was observed. Corynebacterium parvum activated peritoneal adherent cells caused 80% DNA synthesis inhibition of tumor cells while Pustulan activated peritoneal cells showed very low (26%) cytostatic effect.
D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D008175:Lung Neoplasms / Q000628:therapy*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009374:Neoplasms, Experimental / Q000628:therapy; D009929:Organ Size / Q000187:drug effects; D011134:Polysaccharides / Q000627:therapeutic use*; D011425:Propionibacterium acnes / Q000276:immunology; D013154:Spleen / Q000187:drug effects; D013997:Time Factors
D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents; D001428:Bacterial Vaccines; D011134:Polysaccharides; C002076:pustulan