Mutagenicity and chemical analysis of aliphatic and aromatic fractions of Prudhoe Bay crude oil and fuel oil no. 2.
The mutagenicity of aliphatic (fraction 1), one- to three-ring aromatic (fraction 3), and four- to five-ring aromatic (fraction 4) fractions of Prudhoe Bay crude oil and of a fuel oil no. 2 was investigated with a battery of in vitro mammalian and microbial assays. Chemical analysis of the fractions was carried out by gas capillary chromatography-mass spectrometry. In the Salmonella/mammalian microsome test, both first fractions were negative, while fraction 3 of Prudhoe Bay crude and fraction 4 of both oils exhibited mutagenic activity with metabolic activation. In Chinese hamster ovary cells, all of the aromatic fractions, in the presence of metabolic activation, caused a significant increase in the number of sister chromatid exchanges. Chromosome aberrations were not caused by any of the aromatic fractions; however, fraction 3 of fuel oil no. 2 induced endoreduplication in the presence of metabolic activation.
D000818:Animals; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D005648:Fuel Oils / Q000032:analysis / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D010053:Ovary / Q000166:cytology; D010578:Petroleum / Q000633:toxicity*; D051381:Rats; D012475:Salmonella / Q000187:drug effects; D012854:Sister Chromatid Exchange / Q000187:drug effects
D005648:Fuel Oils; D009153:Mutagens; D010578:Petroleum