[Changes in membrane permeability following a rapid increase in the density of a lymphoid cell suspension].
Incorporation of uridine into the cells was studied under conditions of rapid increase in density of the cell suspension either freshly-prepared from spleen of mice strain C57BI/6 or subjected to precultivation. Absorption of uridine by the cells after an increase in their density was rapidly decreased within several minutes. The phenomenon was not due to presence of dead cells in the culture. An inhibitor of protein synthesis puromycin partially removed the suppression of uridine absorption in dense cultures.
D000818:Animals; D002452:Cell Count; D002463:Cell Membrane Permeability / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D007930:Leucine / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D011691:Puromycin / Q000494:pharmacology; D013154:Spleen / Q000166:cytology* / Q000378:metabolism; D014529:Uridine / Q000378:metabolism*
D003470:Culture Media; D011691:Puromycin; D007930:Leucine; D014529:Uridine
Izmenenie pronitsaemosti membran pri bystrom uvelichenii plotnosti suspenzii limfoidnykh kletok.