[Reduction of nitrazepam by rat liver mitochondria (author's transl)].
Highly purified rat liver mitochondria are able to reduce nitro groups of organic compounds to primary amines. This reductase-activity is bound to mitochondrial inner membrane or its matrix. It has no plain substrate specificity whereas it will attack the various substrates at widely different rates [nitrazepam (Mogadan) 1.7 times faster than 4-nitrobenzoic acid]. The activity was markedly inhibited under aerobic conditions. The rate of nitrazepam reduction by rat liver mitochondria was about 1/3--1/4 the rate by microsomal fraction, provided that both preparations contained the same protein amounts. NADH was required as a cofactor, but the nitroreduction was also stimulated by some Krebs cycle intermediates, especially by alpha-ketoglutarate. The reaction was markedly inhibited by organic solvents, even by little volumes. DMSO and DMF are cancelling every activity.
D000818:Animals; D001711:Biotransformation; D003067:Coenzymes / Q000378:metabolism; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D009567:Nitrazepam / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats
D003067:Coenzymes; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System; D009567:Nitrazepam
Reduktion von Nitrazepam durch Rattenleber-Mitochondrien.