The effect of dietary restriction on cell division potential, DNA content and enzyme levels in the rotifer Asplanchna brightwelli.
Dietary restriction in the rotifer Asplanchna brightwelli, carried out by lengthening the interval between feedings, causes an increase both in mean lifespan and in the length of the reproductive period. In the present study, we found that the rate of nuclear division in the gastric glands and vitellarium of the rotifer, as determined by daily nuclear counts, was retarded by dietary restriction. Spectro-fluorimetric measurements show that total DNA content remains constant from the beginning to the end of the lifespan, and is unaffected by dietary restriction. Lactic dehydrogenase and malic dehydrogenase activities were also measured throughout the lifespan of the rotifer, and were not affected by dietary restriction.
D000375:Aging; D000818:Animals; D002455:Cell Division; D004247:DNA / Q000032:analysis; D004032:Diet; D010088:Oxidoreductases / Q000032:analysis; D012403:Rotifera / Q000166:cytology / Q000201:enzymology / Q000502:physiology*
D004247:DNA; D010088:Oxidoreductases