The reaction of porcine colipase a with tetranitromethane. Generation of extrinsic cotton effects in the visible region.
Porcine pancreatic colipase was reacted with a large excess of tetranitromethane in a two-phase system. Modification of tyrosine to produce nitrotyrosine generates extrinsic Cotton effects near 410 nm in the visible region which are not affected by submicellar concentrations of taurodeoxycholate. On the other hand, supramicellar concentrations specifically altered the spectrum near 350 nm. The total reaction products included both inter- and intramolecular cross-links and the initial mixture can be separated into monomers and polymers (dimers). Despite the complicated reactions that have taken place, the monomer has maintained full activity in a tributyrin-taurodeoxycholate assay system and the evidence supports a more indirect role for tyrosine in colipase interface reactions.
D000818:Animals; D002942:Circular Dichroism; D003089:Colipases; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D008697:Methane / Q000031:analogs & derivatives; D010179:Pancreas / Q000032:analysis; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation; D011506:Proteins; D013552:Swine; D013657:Taurodeoxycholic Acid / Q000494:pharmacology; D013774:Tetranitromethane; D014443:Tyrosine
D003089:Colipases; D046911:Macromolecular Substances; D011506:Proteins; D014443:Tyrosine; D013657:Taurodeoxycholic Acid; D013774:Tetranitromethane; D008697:Methane