Programmed flow preparation of DNA-oligomers.
A new liquid phase preparation system for the phosphotriester method has been developed in which the usual batch process is carried out by flow manipulations. All processes including reactions, extraction and chromatography separation were performed in glass and PTFE tubing through which the solution was passed along a programmed course controlled by valves. A micro-computer programme was compiled for automatic control of the entire system.
D001483:Base Sequence; D004247:DNA; D007202:Indicators and Reagents; D008722:Methods; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009841:Oligonucleotides / Q000138:chemical synthesis*
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D004247:DNA