[Preparation of a polyvalent antiserum specific for human muscle proteins. I. Muscle extraction: a macromethod and a micromethod].
Protein metabolism may be upset dramatically by several pathological condition such as starvation, malnutrition, surgical stress. Serum proteins have been investigated in many works but very few studies exist about muscle and organ proteins. This article describes two methods for extraction of muscle water soluble proteins: a macromethod that uses some hundred grams of tissue, and a micromethod that starts from a little muscle biopsia. Extracts have been tested for protein content by electrophoresis on cellulose acetate and isoelectrofocusing on polyacrylamide gel and have shown a rich variety of protein fractions.
D000818:Animals; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D007106:Immune Sera; D007525:Isoelectric Focusing / Q000379:methods; D008832:Microchemistry; D009124:Muscle Proteins / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits / Q000276:immunology
D007106:Immune Sera; D009124:Muscle Proteins
Preparazione di un antisiero polivalente specifico per le proteine muscolari umane. I. Preparazione dell'estratto muscolare: un macrometodo ed un micrometodo.