Characterization of a new potent heparin. 4th communication: The effect of a new potent heparin preparation on lipid components and leucocytes in rats after subcutaneous application.
The effect of a new potent heparin preparation on blood lipid components and leucocytes after a single subcutaneous application in rats was investigated and compared with a commercially available heparin. The analysis of plasma cholesterol, glycerol, triglycerides and free fatty acids as well as leucocyte counts and differential counts were performed 1, 2, 4 and 8 h after heparin application. The main effects observed were a marked increase in free fatty acids with a maximum already after 1 h and a state of leucocytosis with a maximum and 4 h after application, besides other minor changes in blood lipid components. However, these effects were less pronounced after a dose of the new heparin of 16.6 mg/kg, which is equipotent with 5000 USP units of the commercially available heparin used in this study on anticoagulant activity on the basis of factor Xa inactivation assay. The biological relevance of the increase in the plasma free fatty acids and the mechanism of heparin induced leucocytosis are discussed.
D000818:Animals; D002784:Cholesterol / Q000097:blood; D005260:Female; D005990:Glycerol / Q000097:blood; D006493:Heparin / Q000008:administration & dosage / Q000494:pharmacology*; D007279:Injections, Subcutaneous; D007962:Leukocytes / Q000187:drug effects*; D008055:Lipids / Q000097:blood*; D051381:Rats; D014280:Triglycerides / Q000097:blood
D008055:Lipids; D014280:Triglycerides; D006493:Heparin; D002784:Cholesterol; D005990:Glycerol