A low-cost image analysis system for scanning transmission electron microscopy low-dose images.
An image processing system is described which has been used to analyze STEM images of biological macromolecules. The system has the inherent virtues of being inexpensive, easy to program and self-contained. The operating procedures are described using a particular molecule (earthworm hemoglobin) as an example. We show the versatility of the system by describing addition, Fourier filtration, background subtraction, gray-scale expansion, y-deflection, and contouring of images. Color images are also produced but cannot be reproduced here.
D000818:Animals; D003461:Crystallography; D005583:Fourier Analysis; D006454:Hemoglobins / Q000032:analysis*; D008855:Microscopy, Electron, Scanning; D009835:Oligochaeta
D006454:Hemoglobins