title: Presence of neurophysins I and II in the human pineal gland: comparison with the content of neurohypophyseal hormones.
abstract: We have previously measured the individual content of immunoreactive vasopressin (AVP), oxytocin (OT) and vasotocin (AVT) in 155 human pineal glands, and report here identification and measurement of the neurophysin (Np) content of the same glands, using specific homologous human neurophysin I (HNp I) and neurophysin II (HNp II) radioimmunoassays. Median values for HNp I were for men 47 ng/gland (range, 5 to 1360) and for women 24 ng/gland (range, 5 to 1000); median values for HNp II were respectively 7 ng/gland (range, 2 to 191) and 15 ng/gland (range, 2 to 356) with no significant difference between men and women for HNp I and HNp II but a significant difference (p less than 0.001) between HNp I and HNp II for both sexes. Gel filtration showed that pineal neurophysins were eluted at the same volume as both standard Np and Np from human posthypophyses used as controls. HNp I correlated both with AVP (rs = 0.54 for men and 0.55 for women) and OT (rs = 0.86 for men and 0.57 for women) but not with AVT, while HNp II correlated with AVP (rs = 0.52 for men and 0.53 for women) and OT (rs = 0.92 for men and 0.50 for women) but not with AVT. This study thus confirms the presence of two neurophysins in the human pineal gland and further indicates that they are related to AVP and OT concentrations in the same gland. The results also imply, however, that the presence of immunoreactive AVT (more probably a closely related peptide) is independent of the neurophysins.
mesh_terms: D001127:Arginine Vasopressin / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D009481:Neurophysins / Q000032:analysis*; D009928:Organ Specificity; D010121:Oxytocin / Q000032:analysis; D010870:Pineal Gland / Q000032:analysis*; D010904:Pituitary Gland, Posterior / Q000032:analysis*; D011863:Radioimmunoassay; D012737:Sex Factors; D014668:Vasotocin / Q000032:analysis
chemical_list: D009481:Neurophysins; D001127:Arginine Vasopressin; D010121:Oxytocin; D014668:Vasotocin