Distribution of spermatozoa in the utero-tubal junction and isthmus of pigs, and their relationship with the luminal epithelium after mating: a scanning electron microscope study.
The epithelial cell morphology and distribution in the utero-tubal junction and isthmus of pigs was documented by scanning electron microscopy around ovulation. In animals mated at different times before slaughter, our observations confirmed that the utero-tubal junction and posterior part of the isthmus regulate the transport of spermatozoa. The utero-tubal junction appears clearly as a form of mechanical valve strongly limiting the number of sperm cells penetrating the oviduct. The isthmus, and especially its posterior part poor in ciliated cells, is a storage place for spermatozoa which appear as though trapped in the epithelial folds. It remains to be demonstrated if they stay in such reservoirs due to the constriction of the lumen by the thick muscular wall of the duct, or to some chemotactic attraction by tubal secretions, or simply due to adhesion on the epithelium. Our study supports the hypothesis that transport of spermatozoa in the isthmus towards the site of fertilization depends in part on ciliary motion. The instant direction of propagation appears random for spermatozoa escaping from the reservoirs. Other factors such as tubal contractions probably ensure that the resultant movement is a progressive ascent.
D000818:Animals; D002923:Cilia / Q000648:ultrastructure; D003307:Copulation / Q000502:physiology*; D005187:Fallopian Tubes / Q000502:physiology / Q000648:ultrastructure*; D005260:Female; D008297:Male; D008855:Microscopy, Electron, Scanning; D013083:Sperm Transport; D013094:Spermatozoa / Q000502:physiology; D013552:Swine / Q000033:anatomy & histology / Q000502:physiology*; D014599:Uterus / Q000502:physiology / Q000648:ultrastructure