Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture.
To monitor the interaction of cell surface acetylcholine (AcCho) receptors with the cytoskeleton, cultured muscle cells were labeled with radioactive or fluorescent alpha-bungarotoxin and extracted with Triton X-100, using conditions that preserve internal structure. A significant population of the AcCho receptors is retained on the skeletal framework remaining after detergent extraction. The skeleton organization responsible for restricting AcCho receptors to a patched region may also result in their retention after detergent extraction.
D000818:Animals; D002454:Cell Differentiation; D002642:Chick Embryo; D003599:Cytoskeleton / Q000502:physiology*; D003902:Detergents; D008565:Membrane Proteins / Q000378:metabolism; D008854:Microscopy, Electron; D008870:Microtubules / Q000502:physiology; D009132:Muscles / Q000196:embryology*; D011485:Protein Binding; D051381:Rats; D011950:Receptors, Cholinergic / Q000378:metabolism*; D012995:Solubility
D003902:Detergents; D008565:Membrane Proteins; D011950:Receptors, Cholinergic