Changes in the pattern of endometrial protein synthesis during decidualization in the rat.
Ovariectomized rats were given hormonal treatment mimicking progestational ovarian secretions. At maximal sensitivity a decidual reaction was induced by scratching the endometrium. After incubation of tissue with [35S]methionine, proteins were extracted from decidualizing and control endometrium and submitted to two-dimensional polyacrylamide gel electrophoresis (pH range 5--7), followed by staining or autoradiography. A total of about 800 different peptides could be distinguished on the gels. By 24 h after the decidual stimulus the most prominent changes were the appearance of 4 new peptides (mol. wt 12 000, 25 000, 42 000 and 56 000) and the disappearance of 5 others (mol. wt 31 000, 35 000, 100 000, 110 000 and 140 000). This new pattern remained grossly unchanged up to 72 h after decidual induction.
D000818:Animals; D002369:Castration; D003656:Decidua / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D011247:Pregnancy; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains