Demonstration of species difference of placental alkaline phosphatase isozymes in acetone-fixed paraffin-embedded tissues.
The best preservation and discrimination of alkaline phosphatase isozymes by means of amino acid inhibition and heat treatment was obtained in sections of acetone-fixed paraffin-embedded tissues as compared to tissues fixed either in 95% ethanol or standard formalin. The results are illustrated in a study differentiating the isozymes of human and mouse placenta.
D000096:Acetone; D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D000431:Ethanol; D005260:Female; D005404:Fixatives; D005557:Formaldehyde; D006651:Histocytochemistry; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D051379:Mice; D010920:Placenta / Q000201:enzymology* / Q000648:ultrastructure; D011247:Pregnancy; D013045:Species Specificity
D005404:Fixatives; D007527:Isoenzymes; D000096:Acetone; D005557:Formaldehyde; D000431:Ethanol; D000469:Alkaline Phosphatase