Gas chromatographic study of cellular fatty acids of comma-shaped bacteria isolated from the vagina.
Gas chromatographic analyses were performed of the cellular fatty acids of 16 strains of comma-shaped bacteria isolated from the vagina of women with discharge. Acidic methanolysis of lyophilized bacterial cells was used, followed by splitless injection of hexane extracts onto a fused silica capillary column. Myristic (14:0), hexadecanoic (16:0), octadecadienoic (18:2), octadecenoic (18:1) and octadecanoic (18:0) acids were the major fatty acids found. Long and short variants of the comma-shaped bacteria showed a great similarity in their fatty acid patterns. No hydroxy acids were detected.
D001419:Bacteria / Q000032:analysis* / Q000166:cytology / Q000302:isolation & purification; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis*; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D005260:Female; D006801:Humans; D009227:Myristic Acids / Q000032:analysis; D009829:Oleic Acids / Q000032:analysis; D010169:Palmitic Acids / Q000032:analysis; D013229:Stearic Acids / Q000032:analysis; D014621:Vagina / Q000382:microbiology*
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D009227:Myristic Acids; D009829:Oleic Acids; D010169:Palmitic Acids; D013229:Stearic Acids; C027369:octadecadienoic acid