title: [Trials to eliminate the sex odor of growing boars through active immunization with an androstenone-protein conjugate].
abstract: Determination of immunogenicity in rabbit was followed by the use of 5 alpha-Androst-16-en-3-on cattle serum albumin conjugate (AC-) in Freund's adjuvant for active immunisation of young growing boars against boar pheromone. Two experiments were undertaken. Five boars, aged 140 days, were involved in the first experiment. They received six AC- doses in intervals of 14 days (4 mg, 5 mg, 6 mg, 7 mg, 8 mg, 9 mg). Each of the doses was applied to four points, two of them subcutaneous and two intramuscular. In the second experiment, four boars, aged 121 days, received 40 mg AC- each in eight applications, among them three injections in intervals of one week, followed by a pause of two weeks, another three injections again in intervals of one week, and two injections in intervals of four weeks. Another group of four animals received 20 mg AC- in four applications in intervals of four weeks. Five control boars were included in the first experiment and four in the second. Immune response of the experimental animals was detected neither by immunoelectrophoresis nor by radio-immuno-assay. Pheromones in fat samples were determined by means of gas chromatography during treatment and after slaughter. They were increased in some of the cases, albeit not significantly. The nuclear diameters of the interstitional cells of Leydig in testicular sections differed but little, with no significance being recorded.
mesh_terms: D000273:Adipose Tissue / Q000032:analysis; D000736:Androstenes / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000937:Antigen-Antibody Reactions / Q000187:drug effects; D004341:Drug Evaluation / Q000662:veterinary; D004353:Drug Evaluation, Preclinical / Q000662:veterinary; D005260:Female; D007114:Immunization / Q000662:veterinary*; D008297:Male; D009812:Odorants / Q000517:prevention & control*; D010675:Pheromones / Q000032:analysis; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D012741:Sexual Maturation / Q000187:drug effects*; D013552:Swine / Q000254:growth & development* / Q000276:immunology; D013737:Testis / Q000187:drug effects; D013997:Time Factors
chemical_list: D000736:Androstenes; D010675:Pheromones; C000214:androst-16-en-3-one; D012710:Serum Albumin, Bovine
vernacular_title: Versuche zur Beseitigung des Geschlechtsgeruches durch aktive Immunisierung von wachsenden Ebern mit Androstenon-Eiweisskonjugat.