Effects of genetically different strains of mice on the microsomal activation of 2-aminofluorene.
Five strains of mice were examined for microsomal cytochrome P450 (Cyt P450) activity and ability of the microsomal preparation to activate 2-aminofluorene (2-AF) to its mutagenic form. Each strain was assayed under normal and induced conditions. Group A strains known to be easily induced to carcinogenesis by chemicals (C57BL/6J and BALB/cJ) showed higher levels of Cyt P450 and increased mutagenesis activity of the metabolized 2-AF than Group B strains which are known to be high spontaneous tumor producers (CE/J, A/HeJ, C3H/HeJ). Induction of the hepatic microsomes with Aroclor 1254 increased the difference between the two groups.
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D001711:Biotransformation; D005449:Fluorenes / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008805:Mice, Inbred A / Q000378:metabolism; D008807:Mice, Inbred BALB C / Q000378:metabolism; D008809:Mice, Inbred C3H / Q000378:metabolism; D008810:Mice, Inbred C57BL / Q000378:metabolism; D008815:Mice, Inbred Strains / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism*; D009152:Mutagenicity Tests; D013045:Species Specificity
D005449:Fluorenes; C012177:2-aminofluorene; D001189:Aryl Hydrocarbon Hydroxylases