Effect of monensin on Mason-Pfizer monkey virus glycoprotein synthesis.
The effect of the monovalent carboxylic ionophore monensin on the biosynthesis, intracellular transport, and surface expression of the glycoproteins of Mason-Pfizer monkey virus was examined. Cells treated with monensin at concentrations of 10(-7) or 10(-6) M continued to synthesize virus particles, which from electron microscopic studies appeared to bud normally from the plasma membrane of the cells. However, the particles released had an altered buoyant density in sucrose gradients and were noninfectious. These noninfectious virions had a normal complement of non-glycosylated polypeptides but showed a significantly reduced amount of glycosylated proteins. The gp70 and gp20 polypeptides appeared to be completely absent, and a heterogeneous, higher-molecular-weight protein was observed on the virions instead. Studies on intracellular protein synthesis indicated that the precursor (Pr86env) to gp70 and gp20 is synthesized normally but is not cleaved to the mature proteins. Immunofluorescence studies showed, however, that the uncleaved molecule is expressed on the cell surface. In this system, therefore, Mason-Pfizer monkey virus glycoprotein migration appears to occur in the presence of monensin, whereas the cleavage and insertion of the glycoproteins into virions are inhibited.
D000818:Animals; D002460:Cell Line; D004622:Embryo, Mammalian; D005260:Female; D005663:Furans / Q000494:pharmacology*; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D007668:Kidney; D008253:Macaca mulatta; D008325:Mammary Neoplasms, Experimental / Q000382:microbiology / Q000648:ultrastructure; D008854:Microscopy, Electron; D008985:Monensin / Q000494:pharmacology*; D011247:Pregnancy; D012190:Retroviridae / Q000187:drug effects / Q000235:genetics* / Q000648:ultrastructure; D014764:Viral Proteins / Q000235:genetics*; D014771:Virion / Q000302:isolation & purification
D005663:Furans; D006023:Glycoproteins; D014764:Viral Proteins; D008985:Monensin