Conversion of 5 alpha-dihydrotestosterone to oestrone and oestradiol by the chick embryo ovary in organ culture.
Ovaries from 17-day-old chick embryos were cultured in vitro in the presence of [3H]-5 alpha-dihydrotestosterone (5 alpha-DHT) and the formation of radiolabelled oestrone and oestradiol was investigated. Using thin-layer chromatography and crystallization to constant specific activity, the formation of both oestrogens has been demonstrated, although the yield may be considered very low. Aromatization of 5 alpha-DHT had not been previously reported. Further studies should be aimed at elucidating the metabolic pathway leading from 5 alpha-DHT to oestrogens. The isolation of the aromatase system should also be attempted.
D000818:Animals; D002642:Chick Embryo; D013196:Dihydrotestosterone / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D004970:Estrone / Q000378:metabolism*; D005260:Female; D008297:Male; D008650:Mesonephros / Q000378:metabolism; D009924:Organ Culture Techniques; D010053:Ovary / Q000378:metabolism*; D013737:Testis / Q000378:metabolism
D013196:Dihydrotestosterone; D004970:Estrone; D004958:Estradiol