Fractionated dose studies with X-rays and various alkylating agents in P388 mouse lymphoma cells.
The fractionated dose technique has been used in P388F cells to examine the effects of X-rays and four alkylating agents on survival and induction of 5-iodo-2-deoxyuridine (IudR) resistant variants. Fractionation intervals up to 5 1/2 h were used for X-rays and for the alkylating agents up to 192 h. Fractionation of the X-ray dose resulted in a sparing effect for survival and variant induction. A sparing effect was also observed for survival after treatment with alkylating agents. However, variant frequencies were observed as large as or greater than those produced by the full doses of alkylating agents. For such agents this would suggest that survival and variant induction are independent events. Differences in the effects of X-rays and alkylating agents cannot be explained by differences in growth rate or the recovery of viability after treatment.
D000477:Alkylating Agents / Q000494:pharmacology*; D000818:Animals; D007941:Leukemia P388 / Q000503:physiopathology*; D007942:Leukemia, Experimental / Q000503:physiopathology*; D009154:Mutation / Q000187:drug effects / Q000528:radiation effects; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology; D013997:Time Factors; D014965:X-Rays
D000477:Alkylating Agents; D009588:Nitrogen Mustard Compounds