Quantitation of six androgens by combined high performance liquid chromatography and radioimmunoassay.
A technique was developed to separate six androgens (testosterone, dihydrotestosterone, androstenedione, 5 alpha-androstane-3 alpha, 17 beta-diol, 5 alpha-androstane-3 beta, 17 beta-diol, and androsterone) by high performance liquid chromatography prior to quantitation by specific radioimmunoassay systems. Methanol:water (60:40 v:v) was used as the solvent system with a C18 reversed-phase column. The method was verified and used to quantitate the androgens in serum from adult rams bled every 20 minutes for 6 hours and yearling bulls bled every 30 minutes for 8 hours. Concentrations of all 6 androgens varied in an episodic manner with testosterone being the dominant androgen.
D000728:Androgens / Q000097:blood*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002940:Circadian Rhythm; D008297:Male; D011863:Radioimmunoassay / Q000379:methods; D012756:Sheep; D013329:Structure-Activity Relationship
D000728:Androgens