[Biosynthesis and phosphorylation of chromatin, cytoplasmic and cell membrane proteins of the rat neocortical neurons and glial cells in a normal state and during various functions].
Intensive motor activity strongly changes the biosynthesis and enzymatic modification of proteins (phosphorylation) in the rat brain neocortex cells. For different groups of neuronal and glial cell proteins, this change is different. For proteins of the neuron cytoplasm, under the above functional influences a decrease of biosynthesis and phosphorylation occurs in the majority of the fractions examined, having different polyacrylamide gel electrophoretic mobility. In the glial cytoplasm proteins, the situation is reverse. In contrast to the cytoplasmic proteins, the cell membrane proteins of neocortical neurones displayed a high increase in the biosynthesic rate and phosphorylaion. These changes in glial cells were much less and not homogeneous. The functional influences were shown to change not only the velocity of metabolism and phosphorylation, but also the pattern of chromatin protein distribution. Under the functional influences, some new peptide fractions appear in preparations of neocortical neurons.
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D008715:Methionine / Q000378:metabolism; D009043:Motor Activity / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains; D013312:Stress, Physiological / Q000378:metabolism
D002843:Chromatin; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010710:Phosphates; D008715:Methionine
Biosintez i fosforilirovanie belkov khromatina, tsitoplazmy i kletochnykh membran neĭronov i glial'nykh kletok neokorteksa krys v norme i pri funktsional'nykh vozdeĭstviiakh.