Determination of SHBG-bound sex hormones by selective ammonium sulphate precipitation.
This paper describes a direct method for determining sex hormone binding globulin (SHBG)-bound sex hormones in human plasma after separation of SHBG-bound and unbound hormone fractions by selective precipitation with ammonium sulphate. In normal women variations in SHBG-bound and -free hormone generally paralleled fluctuations in total hormone. Changes in SHBG-free estradiol did not have any marked effect on plasma SHBG and sHBG-free testosterone. Our results suggest a buffer role for SHBG through which the biological response to sudden changes in sex hormone concentration is moderated.
D000328:Adult; D000645:Ammonium Sulfate; D011232:Chemical Precipitation; D004958:Estradiol / Q000097:blood*; D005260:Female; D006801:Humans; D008598:Menstruation; D011485:Protein Binding; D012709:Serum Albumin / Q000032:analysis; D012738:Sex Hormone-Binding Globulin / Q000032:analysis*; D013739:Testosterone / Q000097:blood*
D012709:Serum Albumin; D012738:Sex Hormone-Binding Globulin; D013739:Testosterone; D004958:Estradiol; D000645:Ammonium Sulfate