Induction of sister-chromatid exchanges and chromosome aberrations by gamma-irradiated nucleic acid constituents in CHO cells.
Chinese hamster ovary (CHO) cells were exposed to different concentrations of hydrogen peroxide and to gamma-irradiated, oxygenated solutions of thymine, thymidine and 2-deoxy-D-ribose. By using a modified BrdUrd-labelling method sister-chromatid exchanges (SCEs) and chromosomal aberrations were scored in the same cell populations, one cycle after treatment. Irradiated, oxygenated solutions of 2-deoxy-D-ribose clearly induced SCEs and chromosomal aberrations. In comparison, hydrogen peroxide and irradiated solutions of thymine and thymidine were less effective in CHO cells.
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D003434:Crossing Over, Genetic; D003855:Deoxyribose / Q000494:pharmacology* / Q000528:radiation effects; D005260:Female; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D010053:Ovary; D012854:Sister Chromatid Exchange; D013936:Thymidine / Q000494:pharmacology*; D013941:Thymine / Q000494:pharmacology* / Q000528:radiation effects
D003855:Deoxyribose; D006861:Hydrogen Peroxide; D013941:Thymine; D013936:Thymidine