title: Increase of sister chromatid exchange and unscheduled synthesis of deoxyribonucleic acid by acrylonitrile in human lymphocytes in vitro.
abstract: The investigation has been carried out on cultures grown in the presence of 5 x 10(-1) - 5 x 10(-5) M acrylonitrile with or without a rat liver metabolizing system (S-9 mix). The results obtained showed that acrylonitrile was toxic starting from the 5 x 10(-3) M concentration, caused a significant increase in the sister chromatid exchange (SCE) frequency in comparison with the controls (p less than or equal to 0.001) when the concentration was 5 x 10(-4) M, and elicited reparative deoxyribonucleic acid (DNA) synthesis, determined by tritiated thymidine uptake, particularly when the concentration was 5 x 10(-1) M. These effects were observed in lymphocytes of different donors and after drug activation by the S-9 mix metabolizing system.
mesh_terms: D000181:Acrylonitrile / Q000494:pharmacology*; D000328:Adult; D002478:Cells, Cultured; D003434:Crossing Over, Genetic / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair / Q000187:drug effects*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D009152:Mutagenicity Tests; D009570:Nitriles / Q000494:pharmacology*; D009787:Occupational Medicine; D012854:Sister Chromatid Exchange / Q000187:drug effects*
chemical_list: D009570:Nitriles; D004247:DNA; D000181:Acrylonitrile