Sensitizing activity to egg protein of an A1PO4-adjuvant full-virus influenza vaccine.
The sensitizing activity to egg protein of an A1PO4-adjuvant purified and concentrated influenza-A vaccine was examined in animal experiments and in man. Intravenous injection of ovalbumin caused anaphylactic symptoms and/or fatal anaphylactic shock in prevaccinated guinea-pigs. Ovalbumin-specific antibodies detectable by the passive haemagglutination reaction (PHA) appeared in the blood serum of the vaccinated animals. Model experiments with purified ovalbumin suggested that 1 human dose of the vaccine contained egg protein in the range from 0.1 to 1 ng, and that the antigenic effect of the vaccine grew to more than 10(3)-fold by its adsorption to A1PO4 gel. Adults who in previous years had been immunized with similarly prepared influenza vaccine several times responded with mild reactions; symptoms suggestive of hyperergy did not occur, irrespective of the vaccination history. In the prevaccination serum sample of some vaccines, ovalbumin-specific PHA antibodies were found up to titres independent of the number of the previous immunizations. The concentration of the ovalbumin-specific antibodies of the IgE class was by several orders of magnitude lower in the postvaccination samples than in the serum of some patients hypersensitive to egg protein.
D000276:Adjuvants, Immunologic / Q000276:immunology; D000293:Adolescent; D000328:Adult; D000368:Aged; D017607:Aluminum Compounds; D000707:Anaphylaxis / Q000276:immunology; D000818:Animals; D002642:Chick Embryo; D002648:Child; D002675:Child, Preschool; D004527:Egg Proteins / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D006388:Hemagglutinins / Q000032:analysis; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D007223:Infant; D007252:Influenza Vaccines / Q000009:adverse effects* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010047:Ovalbumin / Q000276:immunology; D010710:Phosphates / Q000276:immunology
D000276:Adjuvants, Immunologic; D017607:Aluminum Compounds; D004527:Egg Proteins; D006388:Hemagglutinins; D007252:Influenza Vaccines; D010710:Phosphates; D007073:Immunoglobulin E; D010047:Ovalbumin; C012714:aluminum phosphate