[Changes in the morphology and cytoskeleton of cultured fibroblasts as a result of raised osmotic pressure].
The effect of high osmotic pressure on the morphology and organization of the cytoskeleton and cell-to-substrate contacts of normal mouse embryonic fibroblasts was studied. One-hour incubation of the cells in a hypertonic medium made by the addition of 0.25 M or 0.5 M D-sorbitol to the culture medium leads to the changes in the morphology and to the reduction of the substrate area occupied by cellular lamella. These changes are accompanied by partial destruction of stress fibers and by redistribution of microtubules. Long-term (24 h) incubation of fibroblasts in the presence of 0.25 M sorbitol results in complete normalization of cellular shape. Microfilament bundles in these cells are, however, much thinner and shorter than those in control fibroblasts. An important structural feature of these cells is an actin-containing arc-like band on the periphery of lamella. Interference reflection microscopy reveals almost complete disappearance of "focal contact" at the lower surface of lamellas of these cells. Thus cells exposed for a long time to high osmotic pressure show rearrangement of the cytoskeleton and cell-to-substrate contacts, while retaining normal morphology.
D000818:Animals; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000648:ultrastructure; D005347:Fibroblasts / Q000648:ultrastructure*; D051379:Mice; D008809:Mice, Inbred C3H / Q000196:embryology; D008855:Microscopy, Electron, Scanning; D009994:Osmolar Concentration
Izmeneniia morfologii i tsitoskeleta kul'tiviruemykh fibroblastov pod vliianiem povyshennogo osmoticheskogo davleniia.