title: Glucose transport carrier of human erythrocytes. Radiation target size measurement based on flux inactivation.
abstract: Intact human erythrocytes frozen in the presence of cryoprotective reagents and irradiated with an electron beam retained their diffusion barrier to L-glucose. The carrier-mediated flux of D-glucose, on the other hand, was inactivated as a simple exponential function of the radiation dose. Classical target size analysis of this data yielded a molecular size of 185,000 daltons for the carrier. This represents the first measurement of the functional size of a transport protein based directly on flux inactivation.
mesh_terms: D001693:Biological Transport, Active; D001786:Blood Glucose / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism* / Q000528:radiation effects; D002463:Cell Membrane Permeability; D003571:Cytochalasin B / Q000494:pharmacology; D004058:Diffusion; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007536:Isomerism; D007700:Kinetics; D008757:Methylglucosides / Q000097:blood*; D008759:Methylglycosides / Q000097:blood*; D008970:Molecular Weight; D009004:Monosaccharide Transport Proteins; D009005:Monosaccharides / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D001786:Blood Glucose; D002352:Carrier Proteins; D008757:Methylglucosides; D008759:Methylglycosides; D009004:Monosaccharide Transport Proteins; D009005:Monosaccharides; D003571:Cytochalasin B