Cell-free conversion of isopenicillin N into deacetoxycephalosporin C by Cephalosporium acremonium mutant M-0198.
In a cell-free system prepared by lysis of protoplasts of Cephalosporium acremonium mutant M-0198, isopenicillin N was converted into a penicillinase-resistant material that behaved like deacetoxycephalosporin C on high-pressure liquid chromatography analysis. This activity was found to be unstable to storage at -80 degrees C; 70-80% of the activity was lost after 1 day.
D000164:Acremonium / Q000201:enzymology / Q000378:metabolism*; D002511:Cephalosporins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D010406:Penicillins / Q000378:metabolism*
D002511:Cephalosporins; D010406:Penicillins; C025578:deacetoxycephalosporin C; C006414:penicillin N