Bile acid uptake by isolated intestinal mucosa cells of guinea pig.
Isolated intestinal mucosa cells of the guinea pig were employed to study intestinal transport of bile acids. Chenodeoxycholate and lithocholate were rapidly taken up into jejunal and ileal cells by diffusion. Taurocholate and cholate however showed only a minor diffusion rate and were preferentially taken up by the ileal bile acid carrier. This uptake was saturable with an apparent Km of 231 microM and V of 7 nmol/mg protein per min for taurocholate; this bile acid was accumulated 90-fold. Its uptake was strongly inhibited by antimycin A, FCCP, ouabain or Na+ -deficiency in the medium. Sugars or amino acids did not interfere with uptake. Experimental conditions were optimized with regard to incubation medium, cell amount, cell age and length of preincubation. It is concluded that ileal cells of the guinea pig are superior to other experimental models for characterizing the ileal bile acid carrier, because they allow us to determine initial rates of uptake and have a very efficient energetic coupling.
D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D001692:Biological Transport; D002250:Carbon Radioisotopes; D002635:Chenodeoxycholic Acid / Q000378:metabolism; D006168:Guinea Pigs; D007082:Ileum / Q000378:metabolism; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D007700:Kinetics; D008095:Lithocholic Acid / Q000378:metabolism; D008297:Male; D013656:Taurocholic Acid / Q000378:metabolism; D014316:Tritium
D001647:Bile Acids and Salts; D002250:Carbon Radioisotopes; D002635:Chenodeoxycholic Acid; D014316:Tritium; D013656:Taurocholic Acid; D008095:Lithocholic Acid