Effects of linoleic acid on capping, lectin mediated mitogenesis, surface antigen expression, and fluorescent polarization in lymphocytes and BHK cells.
The incubation of linoleic acid with cells causes profound effects on membrane asociated phenomenon. Using the fluorescent probe diphenyl hexatriene (DPH) to monitor lipid changes in the microenvironment of the cell surface, we find that linoleic acid reduces the polarization values (P) in mouse lymphocytes and BHK cells. Measurements on lipids extracted from the cells grown in linoleic acid produce similar results. We also find in the mouse lymphocyte that capping of Ig is inhibited and con A stimulated mitogenesis is unaffected. In contrast to the latter effect, LPS and PHA stimulated mitogenesis is inhibited and in the rat lymph node, con A stimulated mitogenesis, greatly enhanced. We also show that linoleic acid alters the binding of antibodies to the cell surface of EL-4 lymphoma cells. These observations suggest that linoleic acid alters cellular function by interfering with protein/lipid interactions within the surface membrane.
D000818:Animals; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000276:immunology*; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D006224:Cricetinae; D005454:Fluorescence Polarization; D006801:Humans; D007152:Immunologic Capping / Q000187:drug effects*; D007668:Kidney; D008041:Linoleic Acids / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008563:Membrane Lipids / Q000502:physiology*; D051379:Mice; D010835:Phytohemagglutinins / Q000494:pharmacology
D000954:Antigens, Surface; D008041:Linoleic Acids; D008070:Lipopolysaccharides; D008563:Membrane Lipids; D010835:Phytohemagglutinins; D003208:Concanavalin A