Preparation of specific antiserum to estradiol 3-glucuronide.
The preparation and antigenic properties of estradiol 3-glucuronide-bovine serum albumin (BSA) conjugate in which the hapten is coupled to the carrier protein through the aminomethyl group at C-6 by glutaraldehyde, have been described. Antibody raised against the immunogen in the rabbit possessed high affinity (Ka = 3.7 x 10(9) M-1) and excellent specificity to estradiol 3-glucuronide, exhibiting no significant cross-reactivities with estrogen glucuronides except for estriol 3-glucuronide (3.64%) and no cross-reactions with free estrogens, their sulfates and other steroids.
D000818:Animals; D000918:Antibody Specificity; D003429:Cross Reactions; D004958:Estradiol / Q000031:analogs & derivatives* / Q000276:immunology; D007106:Immune Sera; D007700:Kinetics; D008297:Male; D011817:Rabbits / Q000276:immunology
D007106:Immune Sera; C025482:estradiol-3-glucuronide; D004958:Estradiol