title: Immunogenicity of leptospiral vaccines grown in protein-free medium.
abstract: Leptospira interrogans serovars pomona and hardjo were adapted to grow in a chemically-defined, protein-free (PF) medium. Formolised monovalent vaccines of serovars pomona and hardjo and a bivalent mixture of the two were prepared from PF cultures. Live PF cultures and the vaccine preparations retained their agglutinating antigens and their immunogenicity when tested in rabbits and guinea-pigs. The vaccines were not pyrogenic and dermal reactions were slight.
mesh_terms: D000373:Agglutinins / Q000032:analysis; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D003470:Culture Media; D005557:Formaldehyde; D006168:Guinea Pigs; D007919:Leptospira / Q000276:immunology*; D011817:Rabbits; D012882:Skin Tests
chemical_list: D000373:Agglutinins; D001428:Bacterial Vaccines; D003470:Culture Media; D005557:Formaldehyde