title: Measurement of adenosine triphosphate and some other metabolites in blood cells by isotachophoresis. I. Preparative technique and enzymatic confirmation.
abstract: This study investigated the optimal conditions for detection of nucleotides in blood using an IP-1B capillary isotachophoretic apparatus. The system used 10 mM HCl-beta-alanine (pH 4.2) as the leading electrolyte and n-caproic acid as the terminal electrolyte. Direct application of lysed red blood cells was shown to be inaccurate, and a method of deproteinization based on heat in a microwave oven was developed. The zones for 2,3-diphosphoglycerate, ATP, inorganic phosphate, and lactate were identified enzymatically by withdrawal of pure samples of each zone via a special withdrawal cell. The quantitative values obtained by isotachophoresis were also confirmed enzymatically. The technique is now available for convenient and accurate identification of these metabolites simultaneously.
mesh_terms: D000244:Adenosine Diphosphate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood*; D002951:Citrates / Q000097:blood; D019343:Citric Acid; D004163:Diphosphoglyceric Acids / Q000097:blood; D004586:Electrophoresis / Q000379:methods; D004912:Erythrocytes / Q000032:analysis*; D006801:Humans
chemical_list: D002951:Citrates; D004163:Diphosphoglyceric Acids; D019343:Citric Acid; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate