[Practical use of the Multistat III centrifuge analyzer in an improved method for the determination of the activity of aspartate aminotransferase and alanine aminotransferase].
We applied three procedures for measuring aspartate aminotransferase and alanine aminotransferase activities to the Multistat III MCA. Assay conditions were almost exactly as prescribed by the Scandinavian Committee on Enzymes and the International Federation of Clinical Chemistry. Owing to the overall good performance of the analyzer and its loader module, results of 20 samples will be available in 10 minutes, including preincubation with pyridoxal phosphate. Details of procedures are given; there are no differences in activities of both aminotransferases determined with three methods, allowing the user to take a choice according to personal preference.
D000410:Alanine Transaminase / Q000032:analysis*; D001219:Aspartate Aminotransferases / Q000032:analysis*; D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods; D002498:Centrifugation / Q000295:instrumentation; D006801:Humans
D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
Applicazione pratica sull'analizzatore centrifugo multistat III della determinazione ottimizzata delle attività aspartato aminotransferasi (AST) ed alanina aminotransferasi (ALT).