Conformational analysis of small disulfide loops. Spectroscopic and theoretical studies on a synthetic cyclic tetrapeptide containing cystine.
The conformational analysis of the synthetic peptide (formula, see text) has been carried out, as a model for small disulfide loops, in biologically active polypeptides. 1H NMR studies (270 MHz) establish that the Val(3) and Cys(4) NH groups are solvent shielded, while 13C studies establish an all-trans peptide backbone. Circular dichroism and Raman spectroscopy provide evidence for a right-handed twist of the disulfide bond. Analysis of the vicinal (J alpha beta) coupling constants for the two Cys residues establishes that chi 1 approximately +/- 60 degrees for Cys(4), while some flexibility is suggested at Cys(1). Conformational energy calculations, imposing intramolecular hydrogen bonding constraints, favor a beta-turn (type I) structure with Pro(2)-Val(3) as the corner residues. Theoretical and spectroscopic results are consistent with the presence of a transannular 4 leads to 1 hydrogen bond between Cys(1) CO and Cys(4) NH groups, with the Val NH being sterically shielded from the solvent environment.
D002942:Circular Dichroism; D003553:Cystine; D004220:Disulfides; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D010456:Peptides, Cyclic; D011487:Protein Conformation; D013059:Spectrum Analysis, Raman
D004220:Disulfides; D010456:Peptides, Cyclic; D003553:Cystine