[Ecto-mesodermal interactions and chick embryo limb chondrogenesis. Ultrastructural studies of cultures in the vitelline membrane (author's transl)].
Stage 17 (Hamburger and Hamilton) limb mesoderm, isolated and cultured in vitro differentiates into cartilage (11.5% of the explants). However, chondrogenesis is considerably improved in the presence of ectoderm, whether the interacting tissues are in close contact or separated by the vitelline membrane. Stage 15-16 limb mesoderm cultured in vitro is unable to differentiate into cartilage. Previous experiments have shown that a contact between ectoderm and mesoderm is necessary for the induction of cartilage to take place. However, when the vitelline membrane separates the two tissues, cartilage differentiation occurs in 16.3% of the explants. Can a contact between the two interacting tissues be established through the vitelline membrane? Ultrastructural studies of the relationships between ectodermal and mesodermal cells and the vitelline membrane have shown that such a contact can be achieved by means of long mesodermal cell processes penetrating deeply into the vitelline membrane.
D000818:Animals; D002356:Cartilage / Q000648:ultrastructure*; D002642:Chick Embryo; D046508:Culture Techniques; D004475:Ectoderm / Q000648:ultrastructure*; D005260:Female; D008648:Mesoderm / Q000648:ultrastructure*; D008854:Microscopy, Electron; D014817:Vitelline Membrane / Q000648:ultrastructure*
Interactions ectoderme-mésoderme en relation avec la chondrogenèse dans le membre de l'embryon de poulet. Etude ultrastructurale en culture dans la membrane vitelline.