Developmental changes in protein generation by cultured porcine granulosa cells.
1. The gross complement of proteins from porcine ovarian follicular fluid does not change as the follicles increase in size. 2. The electrophoretic profile of proteins from porcine follicular fluid is strikingly different from that of plasma proteins from the same animals. 3. Cultured granulosa cells from different sized follicles synthesize and release L-leucine-[3H]labeled proteins at different rates. 4. Cultured porcine granulosa cells appear capable of synthesizing most follicular fluid proteins.
D000818:Animals; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006107:Granulosa Cells / Q000166:cytology* / Q000378:metabolism; D008970:Molecular Weight; D014176:Protein Biosynthesis; D013552:Swine