Base-pair formation between 18 S ribosomal RNA and globin mRNA during initiation of protein synthesis in vitro.
A stable complex between 18 S rRNA and globin mRNA has been isolated from 40 S initiation complexes in the reconstituted reticulocyte cell free system. This complex is only formed under the conditions which also lead to an initiation complex active in protein synthesis. The mRNA-18 S rRNA interaction has properties compatible with base-pairing. This observation is discussed in the context with other, in part controversial, observations relating to base pairing as a step in initiation of eukaryotic protein synthesis.
D002474:Cell-Free System; D005914:Globins / Q000096:biosynthesis; D066298:In Vitro Techniques; D010442:Peptide Chain Initiation, Translational; D010448:Peptide Initiation Factors / Q000494:pharmacology; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D005914:Globins