title: Identification of microtubule-associated proteins in the meiotic spindle of surf clam oocytes.
abstract: Meiotic spindles isolated from surf clam oocytes to morphological purity are biochemically complex, consisting of many polypeptides. These proteins fall into two classes: (a) polypeptides that are apparently cytoplasmic proteins and are not specifically associated with the spindle; and (b) polypeptides that are specifically associated with the spindle. A subset of the spindle-associated proteins, including a 250,000 mol wt component, remain with spindle tubulin through cycles of cold depolymerization and warm polymerization, showing that they are microtubule-associated proteins.
mesh_terms: D000818:Animals; D049872:Bivalvia; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005990:Glycerol; D006018:Glycols; D008540:Meiosis; D008870:Microtubules / Q000032:analysis*; D009698:Nucleoproteins / Q000032:analysis*; D009865:Oocytes / Q000648:ultrastructure*; D009940:Organoids / Q000648:ultrastructure*; D010063:Ovum / Q000648:ultrastructure*; D010455:Peptides / Q000032:analysis; D014404:Tubulin / Q000032:analysis*
chemical_list: D006018:Glycols; D009698:Nucleoproteins; D010455:Peptides; D014404:Tubulin; D005990:Glycerol