AC polarographic determination of cloxacillin.
Hydrolysis of cloxacillin at pH 4.0 yields an electroactive product which can be determined by polarography. Depending on the concentration of cloxacillin, one of 2 peaks was obtained: The potential ac peaks for a 4.36 mM cloxacillin solution were peak I, -0.23 V and peak II, -0.13 V. For analytical purposes, the first peak was used. A linear relationship was established for levels of cloxacillin between 2.2 X 10(-5) and 2.2 X 10(-3)M. Average recovery was 98.8% (SD 1.8), indicating satisfactory accuracy for the method. Individual capsule and composite assays as well as interference tests are described.
D002214:Capsules / Q000032:analysis; D003023:Cloxacillin / Q000032:analysis*; D006868:Hydrolysis; D011048:Polarography / Q000379:methods; D013696:Temperature
D002214:Capsules; D003023:Cloxacillin