Stem cell (CFU-s) proliferation in sublethally irradiated mice.
The proliferation rate of haemopoietic stem cells (CFU-s) was followed after sublethal total body irradiation with 1 X 5 Gy. The [3H]-thymidine suicide technique was used to measure the CFU-s proliferation rate. The measurements extended from 10 min after irradiation up to 21 days. The CFU-s did not enter the DNA synthesis period (S-phase) shortly after irradiation, as had been previously suggested, but did so only with a delay of 14-16 hr. A large scatter of results was explained by an oscillatory pattern in CFU-s proliferation. The CFU-s prepared for cell division in synchronized waves, with a period of 20-22 hr.
D000818:Animals; D002455:Cell Division / Q000528:radiation effects; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000528:radiation effects*; D051379:Mice; D013997:Time Factors