[Review of the methods of determination of blood urea with continuous-flow analyzers and a proposal of a completely enzymatic UV method for urea by a continuous-flow analyzer].
After a brief review of the methods for determination of urea by continuous flow analyzers, a method is described based on the urease splitting of urea followed by NH3 reaction with alfa-ketoglutarate + NADH2 catalysed by GLDH. The method has been applied to continuous flow analyzers and seems to be promising.
D005969:Glutamate Dehydrogenase; D008722:Methods; D014508:Urea / Q000032:analysis / Q000097:blood*; D014510:Urease
D014508:Urea; D005969:Glutamate Dehydrogenase; D014510:Urease
Breve rassegna dei metodi di determinazione della urea plasmatica con analizzatori a flusso continuo e proposta di un metodo UV completamente enzimatico per l'urea su analizzatore a flusso.