[Detection of lymphocytotoxic HLA-A, B, C antibodies in the blood of married female blood donors. I. Serological study].
Bleedings from 1.843 blood donors married women have been checked, according to NIH standard technique, against panels of 17-20 samples of lymphocytes from random healthy HLA-A, -B, -C typed individuals of the Turin population. Lymphocytotoxic activity has been found in 209 sera, but only 90 of them showed strong reaction(s) (at least 60-80% of killed lymphocytes) and 20 of these were HLA typing (or easily transformable in HLA typing) sera. Very few sera killed only 20-50% of lymphocytes in some samples and this cytotoxic activity remained even if dilutions as far as 1:16 and, sometimes, 1:32 were made. Bleedings from blood donors married women give a small yield (1%) of ;typing sera, but these sera are easily available and, practically, without problems of quantity.
D000017:ABO Blood-Group System; D000961:Antilymphocyte Serum; D001782:Blood Donors; D003168:Complement Fixation Tests; D005260:Female; D006680:HLA Antigens / Q000032:analysis*; D006801:Humans; D007518:Isoantibodies / Q000032:analysis*; D008214:Lymphocytes / Q000276:immunology*; D008393:Marriage; D010298:Parity; D012737:Sex Factors
D000017:ABO Blood-Group System; D000961:Antilymphocyte Serum; D006680:HLA Antigens; D007518:Isoantibodies
Ricerca di anticorpi HLA-A, -B, -C linfocitotossici nel siero di donatrici di sangue coniugate. I. Studio sierologico.