[Methods and their evaluation in estimating the vitamin B6-status in humans. 3. Determination- of 4-pyridoxic acid in urine].
A method based on the fluorimetric determination of the 4-PA-lactone is described. The applied method saves time and work compared to others. The analytical procedure may be interrupted for a few days after eluting the 4-PA from the ion exchanger as well as before measuring the transmission. Accuracy, precision and sensitivity of the method are demonstrated. Interfering substances influencing the fluorescence measurement and probable errors caused by the reference value creatinine are discussed. Storage tests applied with the urine samples at--18 degrees C have shown that 4-PA-values do not change considerably within two months.
D002852:Chromatography, Ion Exchange; D006801:Humans; D007539:Isonicotinic Acids / Q000652:urine*; D011735:Pyridoxic Acid / Q000031:analogs & derivatives / Q000652:urine*; D011736:Pyridoxine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods
D007539:Isonicotinic Acids; C036509:4-pyridoxic acid lactone; D011735:Pyridoxic Acid; D011736:Pyridoxine
Methoden und deren Wertung zur Bestimmung des Vitamin-B6-Versorgungszustandes beim Menschen. 3. Mitteilung: Die Bestimmung der 4-Pyridoxinsäure im Harn.