Nucleic acid-binding properties of adenovirus structural polypeptides.
The ability of adenovirus structural polypeptides to bind nucleic acids was assessed by separating the polypeptides on SDS-polyacrylamide gels, transferring them electrophoretically to nitrocellulose and probing with 32P-labelled nucleic acids. Polypeptides IVa2, V, VI and VII, as well as trace amounts of pVII and a polypeptide of apparent mol. wt. 40 X 10(3) were able to bind label under these conditions. Labelling was also detected with a smaller polypeptide, possibly related to the cleavage products of pVII and/or pVI. The binding of DNA to polypeptide VI appeared to be more sensitive to detergents than the others. No sequence specificity could be detected in the DNA binding.
D000260:Adenoviruses, Human / Q000032:analysis*; D004279:DNA, Viral / Q000378:metabolism*; D003840:Deoxycholic Acid / Q000494:pharmacology; D017830:Octoxynol; D009975:Orthomyxoviridae / Q000032:analysis; D010957:Plasmids; D011092:Polyethylene Glycols / Q000494:pharmacology; D011498:Protein Precursors / Q000378:metabolism; D014508:Urea / Q000494:pharmacology; D014764:Viral Proteins / Q000378:metabolism*
D004279:DNA, Viral; D011498:Protein Precursors; D014764:Viral Proteins; D003840:Deoxycholic Acid; D011092:Polyethylene Glycols; D014508:Urea; D017830:Octoxynol