Demonstration of sulfhydryl and disulfide groups by a fluorescent maleimide procedure.
Several fluorescent maleimide compounds were evaluated as possible substitutes for N-(4-aminophenyl)maleimide in the histochemical procedures developed by Sippel (1973, 1978a, b, 1980) for the demonstration of sulfhydryl and disulfide groups. The brightest and most selective fluorescence was obtained by using N-(7-dimethylamino-4-methylcoumarinyl)maleimide (DACM), although both eosin-5-maleimide and fluorescein-5-maleimide could also be used if adequate control preparations were made.
D000818:Animals; D004220:Disulfides / Q000032:analysis*; D004947:Esophagus / Q000032:analysis; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D007422:Intestines / Q000032:analysis; D008301:Maleimides; D008407:Mast Cells / Q000032:analysis; D051379:Mice; D008813:Mice, Inbred ICR; D008856:Microscopy, Fluorescence / Q000379:methods; D010053:Ovary / Q000032:analysis; D013329:Structure-Activity Relationship; D013438:Sulfhydryl Compounds / Q000032:analysis*
D004220:Disulfides; D005456:Fluorescent Dyes; D008301:Maleimides; D013438:Sulfhydryl Compounds