Response of the in vitro whole bladder (rabbit) preparation to autonomic agonists.
Using an in vitro whole bladder preparation (rabbit), we studied both the volume-pressure relationship and the response of the bladder to specific autonomic agonists. We determined the response in: 1) an isovolumetric system in which the intravesical pressure was monitored in the absence of volume changes; 2) a system in which volume alterations were monitored in the presence of increasing pressure; and 3) a system in which the bladder emptied in the presence of a constant pressure. The results of these studies demonstrated: 1) volume-pressure studies generated a typical cystometric curve; 2) bethanechol produced a dose-dependent increase in intravesical pressure (decrease in bladder volume); 3) the whole bladder responded very poorly to isoproterenol; and 4) methoxamine and ATP both produced a modest increase in intravesical pressure. One advantage of the "whole bladder" in vitro preparation is that it can objectively measure a functional response of the bladder to pharmacological agents.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001337:Autonomic Agents / Q000494:pharmacology*; D001626:Bethanechol Compounds / Q000494:pharmacology; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008729:Methoxamine / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects*; D011312:Pressure; D011817:Rabbits; D014160:Transducers, Pressure; D001743:Urinary Bladder / Q000187:drug effects*
D001337:Autonomic Agents; D001626:Bethanechol Compounds; D000255:Adenosine Triphosphate; D008729:Methoxamine; D007545:Isoproterenol