Microfilament reorganization in normal and cytochalasin B treated adherent thrombocytes.
Thrombocyte adhesion following activation by a Formvar surface involves a morphologic transition resulting in a fully spread cell. Correlative SEM and whole mount TEM were used to study the cytoskeletal alterations that accompany changes in surface morphology during adhesion. Following initial adhesion, thrombocytes extend slender pseudopods containing longitudinally oriented bundles of filaments that are 13-22 nm in diameter. Concomitant with pseudopod extension, a cytoplasmic hyalomere, consisting of a dense filamentous network, extends between the pseudopods and ultimately results in a fully spread cell. Treatment of thrombocytes with cytochalasin B (10(-5) M) caused clumping of the hyalomere filament network and retraction of the hyalomere. Examination of partially retracted cells revealed that pseudopod filament bundles were continuous with the contracting filamentous network. It is concluded that pseudopod filament bundles and cytoplasmic hyalomere filaments are interconvertible and that their organizational relationship changes in accordance with gross morphologic changes.
D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D003571:Cytochalasin B / Q000494:pharmacology*; D003599:Cytoskeleton / Q000648:ultrastructure*; D006801:Humans; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010974:Platelet Aggregation / Q000187:drug effects*
D003571:Cytochalasin B