title: The regional distribution of poly (A) and total RNA concentrations during early Xenopus development.
abstract: Xenopus laevis embryos were oriented and sectioned into six regions along one of three axes: animal-vegetal, dorsal-ventral, or right-left. Total RNA (predominantly ribosomal RNA) and poly riboadenylate [poly (A)] were measured for each region during early embryogenesis. The concentrations of both total RNA and poly(A) are nonuniformly distributed during early development. Total RNA is approximately four-fold more concentrated in the more animal portions than the more vegetal portions of the embryo. The dorsal-most region contains a 1.5-fold higher concentration than in the other five regions of the four-celled embryo along the dorsal-ventral axis. Poly(A) concentrations show temporal and regional specific changes in concentration during early development with higher concentrations more generally found in the animal-most regions.
mesh_terms: D000818:Animals; D002451:Cell Compartmentation; D011061:Poly A / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology*
chemical_list: D012333:RNA, Messenger; D012335:RNA, Ribosomal; D011061:Poly A