Surface modification of Beckman Ultra-Clear centrifuge tubes for density gradient centrifugation of lipoproteins.
A simple procedure for coating the interior surface of Beckman Ultra-Clear centrifuge tubes with polyvinyl alcohol is described. The coated tubes are wettable and allow salt solutions to gravity-feed down their sides. This modification of the tubes permits the performance of previously developed density gradient and uniform density ultracentrifugation procedures for lipoprotein fractionation.
D002499:Centrifugation, Density Gradient / Q000295:instrumentation*; D006801:Humans; D008074:Lipoproteins / Q000302:isolation & purification*; D008077:Lipoproteins, LDL / Q000302:isolation & purification; D013055:Spectrophotometry, Infrared
D008074:Lipoproteins; D008077:Lipoproteins, LDL