Two complementation classes of T200 (Ly-5) glycoprotein-negative mutants.
Mutants of murine lymphomas have been isolated which express less than 1 percent of wild-type levels of T200 (Ly-5) glycoprotein on their cell surface but express wild-type levels of other cell-surface antigens tested. These mutants define two genetic complementation classes. The Class A mutant does not synthesize detectable amounts of T200 glycoprotein and is a mutant in either the structural gene coding for the T200 glycoprotein or in a gene acting in cis position to regulate this structural gene. The Class B mutant synthesizes very low levels of T200 glycoprotein and is most simply interpreted as a mutant in a gene acting at some post-transcriptional step necessary for expression of the T200 glycoprotein on the cell surface.--The isolation of T200- mutants by cytotoxic immunoselection suggests that this method is a general way of studying the biosynthesis, regulation and function of cell-surface molecules.
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D003433:Crosses, Genetic; D003602:Cytotoxicity, Immunologic; D005816:Genetic Complementation Test; D006023:Glycoproteins / Q000235:genetics*; D006041:Goats; D007519:Isoantigens / Q000235:genetics*; D051379:Mice; D008806:Mice, Inbred AKR; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009154:Mutation; D010641:Phenotype
D000954:Antigens, Surface; D006023:Glycoproteins; D007519:Isoantigens