[Activation of adenyl cyclase from bovine brain caudate nucleus by synthetic analogs of guanyl nucleotides].
In order to investigate the regulatory properties of dopamine-sensitive adenylate cyclase from bovine brain caudate nucleus some non-hydrolyzed guanyl nucleotides modified at the 8th position of the purine base and at phosphate site were synthesized. The activating effects of these compounds and that of GppCH2p on the membrane preparation of adenylate cyclase were studied. The activating effect of GppCH2p was decreased when the substituents were introduced at the 8th position of the purine base or when its terminal phosphate group was modified. The GDP analog ClCH2ppG-8-NH(CH2)6NHCOCH2Cl whose molecule contains simultaneously two substituents with alkylating chloromethyl groups produced an activating effect. This effect was abolished when the length of the substituent at the 8th position was reduced or one of the chloromethyl groups was lacking. The decrease of adenylate cyclase activation by ClCH2ppG-8-NH(CH2)NH(CH2)Cl in the presence of GDP is suggestive of a specific type of interaction of this compound with the enzyme. It is assumed that the components of the adenylate cyclase system may be covalently linked by this guanyl nucleotide.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002421:Caudate Nucleus / Q000201:enzymology*; D004789:Enzyme Activation; D006150:Guanine Nucleotides / Q000494:pharmacology*; D007700:Kinetics; D013329:Structure-Activity Relationship
D006150:Guanine Nucleotides; D000262:Adenylyl Cyclases
Aktivatsiia adenilattsiklazy khvostatogo iadra mozga byka sinteticheskimi analogami guanilovykh nukleotidov.