Transplantation of human skeletal muscle to nude mice: a sequential morphologic study.
Normal human quadriceps muscle was transplanted into nude mice subcutaneously for periods of 1, 2, 3, 4, 6, and 8 weeks. At 1 week, there was myoblast and myotube formation with thin and thick filaments. After 2 weeks, there was myotube formation with partially cross-striated myofilaments, and formation of dyads or triads. Leptomeres were also noted in some myotubes. By 4 weeks, cross-striation of myofilaments and migration of muscle nuclei to the periphery were observed. Distended sarcoplasmic reticulum and honeycomb structures of tubules were noted between 4 and 8 weeks. This study indicates that mature human muscle can be transplanted successfully into nude mice, providing another tool for the investigation of human neuromuscular disease.
D000818:Animals; D002455:Cell Division; D002462:Cell Membrane / Q000648:ultrastructure; D003470:Culture Media; D004594:Electrophysiology; D006003:Glycogen; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009132:Muscles / Q000166:cytology / Q000473:pathology / Q000637:transplantation* / Q000648:ultrastructure; D012519:Sarcoplasmic Reticulum / Q000648:ultrastructure; D014183:Transplantation, Heterologous
D003470:Culture Media; D006003:Glycogen