Stimulation by potassium of cholesterol ester depletion of rat zona glomerulosa cells in vitro.
The relationship between cholesterol metabolism and steroidogenesis by rat zona glomerulosa cells in vitro was examined by study of the changes in the cellular content of free- and esterified-cholesterol duing basal and K+-stimulated steroidogenesis, K+-stimulation was associated with an increased rate of depletion of cellular esterified-cholesterol, in the absence of any significant change in free-cholesterol levels. These data suggest that cholesterol for steroidogenesis by rat zona glomerulosa cells in vitro is supplied by hydrolysis of endogenous stores of esterified-cholesterol.
D000311:Adrenal Glands / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002788:Cholesterol Esters / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D011188:Potassium / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D002788:Cholesterol Esters; D011188:Potassium