Picornavirus-like inclusions in polymyositis--aggregation of glycogen particles of the same size.
We studied picornavirus-like inclusions in muscle of a 66-year-old man with chronic polymyositis. Inoculation of a muscle homogenate to suckling mice and VeRo cells failed to detect any virus. After treatment of ultrathin sections with 2% amylase, individual particles that constituted inclusions were digested, as were free glycogen particles in the same section. Particles of the inclusions were stained with periodic acid-thiocarbohydrazide-silver proteinate and alkaline-bismuth, as were glycogen particles. The picornavirus-like inclusions seemed to consist of glycogen particles than form a crystalline arrangement in degenerated muscle fibers.
D000368:Aged; D002908:Chronic Disease; D003460:Crystallization; D006003:Glycogen / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D009132:Muscles / Q000378:metabolism / Q000382:microbiology* / Q000648:ultrastructure; D009220:Myositis / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D010849:Picornaviridae
D006003:Glycogen