Oxidative decarboxylation reactions in Dirofilaria immitis glucose metabolism.
1. Malic enzyme (EC 1.1.1.39) activity was demonstrated in the mitochondrial fraction of dog heartworm extracts. 2. Data published earlier together with those presented here confirm the presence of all the enzymes of the pentose shunt. 3. 14C from uniformly labelled glucose was incorporated into the nucleic acids, and methylene blue increased production of xylose-5-phosphate and of 14CO2 from [1-14C]glucose indicating a functioning pathway. 4. The oxidative decarboxylation of malate and of 6-phosphogluconate may account for the observed labelled CO2 formed when labelled glucose is metabolized since a functional tricarboxylic acid cycle is doubtful in this parasitic helminth.
D000818:Animals; D003653:Decarboxylation; D004182:Dirofilaria / Q000187:drug effects / Q000378:metabolism*; D004285:Dogs; D005370:Filarioidea / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000378:metabolism; D008293:Malates / Q000378:metabolism; D008751:Methylene Blue / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology; D010428:Pentosephosphates / Q000378:metabolism
D008293:Malates; D010428:Pentosephosphates; C030298:malic acid; D008291:Malate Dehydrogenase; D005947:Glucose; D008751:Methylene Blue