1H-NMR and photochemically-induced dynamic nuclear polarization studies on bovine pancreatic phospholipase A2.
Proton-NMR resonances of trytophan 3 and tyrosine 69 in bovine pancreatic phospholipase A2, its pro-enzyme and in Ala1-transaminated protein were assigned using photochemically-induced dynamic nuclear polarization (photo-CIDNP) as such or in combination with spin-echo measurements. In addition assignments were made by suppression of cross-relaxation effects using short (0.1 s) high-power laser pulses.
D000818:Animals; D002417:Cattle; D007834:Lasers; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D009686:Nuclear Physics; D010179:Pancreas / Q000201:enzymology*; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D011487:Protein Conformation; D014364:Tryptophan / Q000032:analysis; D014443:Tyrosine / Q000032:analysis
D008823:Micelles; D014443:Tyrosine; D014364:Tryptophan; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2