Quantification of Schistosoma mansoni in mouse lungs by radioassay and autoradiography of 75Se-labeled schistosomula.
Lung radioassay and compressed lung autoradiography of 75Se-labeled schistosomula were used to quantify lung schistosomula. Recovery of schistosomula from minced lung tissue after a 4-hr incubation yielded 69.4 +/- 6.3% of the total number present as demonstrated by subsequent autoradiography of these tissues. Radioassay of lung tissue was considered a valid alternative to autoradiography only for parallel comparisons and those not requiring estimation of the actual number of schistosomula. The number of schistosomula in the lungs on day 6 was significantly correlated with radioactivity in the tail on day 1 (r2 = 91.8%). It was concluded that compressed lung autoradiography provides both an alternative to and an adjunct to standard schistosomulum recovery techniques in the assay of early S. mansoni infections.
D000818:Animals; D001345:Autoradiography; D005260:Female; D007554:Isotopes; D007814:Larva / Q000032:analysis; D008168:Lung / Q000469:parasitology*; D051379:Mice; D012550:Schistosoma mansoni / Q000254:growth & development; D012643:Selenium
D007554:Isotopes; D012643:Selenium