[Valency of antigenic erythrocyte diagnosticums and serum antibody affinity].
The Fab-fragments of antibodies were separated from the intact antibodies by their activity in serological reactions of eluate portins from Sephadex G-100. The valency of antigenic erythrocytic diagnostic agents was evaluated by their maximum ability bind Fab-fragments. The affinity of monovalent Fab-fragments was evaluated with antigenic erythrocytic diagnostic agents. A graphic and an analytical variants of determination of the average association constant by the suspension method have been developed.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D002850:Chromatography, Gel; D004912:Erythrocytes / Q000276:immunology*; D006386:Hemagglutination Tests; D006736:Horses / Q000276:immunology; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology* / Q000302:isolation & purification; D008433:Mathematics; D009500:Neutralization Tests; D010930:Plague / Q000276:immunology*
D000907:Antibodies, Bacterial; D007140:Immunoglobulin Fab Fragments
Valentnost' antigennykh eritrotsitarnykh diagnostikumov i affinitet syvorotochnykh anti tel.