title: Nematode development after removal of egg cytoplasm: absence of localized unbound determinants.
abstract: Embryos of Caenorhabditis elegans develop into fertile adults after cell fragments, containing presumptive cytoplasm of somatic and germ line precursors, are extruded from uncleaved eggs or early blastomeres through laser-induced holes in the eggshells. This suggests that the determinate development of this worm is not dependent on the prelocalization of determinants in specific regions of the egg cytoplasm.
mesh_terms: D000818:Animals; D002107:Caenorhabditis / Q000166:cytology / Q000196:embryology*; D002454:Cell Differentiation; D003593:Cytoplasm / Q000502:physiology*; D005260:Female; D007834:Lasers; D010063:Ovum / Q000502:physiology*