title: Radiolanthanides as markers for vitellogenin-derived proteins in the growing oocytes of the Japanese quail.
abstract: Producing Japanese quail were given intravenous injections of radiolanthanides as trichlorides in .03N HCl solution. In quail given 15 mumol lanthanide pr 100 g body weight, the liver accumulated 46 to 82% of the dose by 18 hr after the injection. The growing oocytes accumulated 6 to 24% of the dose. The spleen had the greatest concentration of radiolanthanide (14 to 38%/g), the liver 9 to 19%/g, and the most heavily labeled oocytes 3 to 9%/g. The atomic number of the lanthanide for the 9 lanthanides used was not a factor in these accumulations. Levels of 140La were maximal in the membranes of the growing oocytes by 6 hr (4%) and in the contents by 2 days (19%). The smaller the number of mumol the greater was the percent accumulation in the oocytes. Thus, for .015 mumol 140La/100 g, 75% was present in the oocytes by 18 hr. The most heavily labeled oocytes contained 21%/g vs. 2.9%/g for the liver and 1.3%/g for the spleen. The sum of 140La depositions in eggs laid over 8 days (11 quail, 87 eggs) was, per quail, 89.6 +/- .6% (mean +/- SE) of the injected dose. The half-time value for deposition of 140La in the growing oocytes was 1.8 hr. This value represented the rate of loss of labeled vitellogenin from the plasma as vitellogenin-derived proteins were transferred into the oocytes.
mesh_terms: D000818:Animals; D001692:Biological Transport; D003370:Coturnix / Q000378:metabolism*; D005260:Female; D008074:Lipoproteins / Q000378:metabolism*; D008674:Metals, Rare Earth; D009865:Oocytes / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D011784:Quail / Q000378:metabolism*; D011868:Radioisotopes; D014817:Vitelline Membrane / Q000378:metabolism; D014819:Vitellogenins / Q000378:metabolism*
chemical_list: D008074:Lipoproteins; D008674:Metals, Rare Earth; D011868:Radioisotopes; D014819:Vitellogenins