Preferential utilization of phenol rather than glucose by Trichosporon cutaneum possessing a partially constitutive catechol 1,2-oxygenase.
A phenol-utilizing yeast, Trichosporon cutaneum POB 14, which has a partially constitutive activity of catechol 1,2-oxygenase, utilized phenol in preference to glucose in a medium containing both phenol (200 mg/liter) and glucose (0.15%) as carbon sources. The glucose consumption was not observed until the concentration of phenol decreased to around 10 mg/liter. This phenomenon was confirmed by [U-14C]glucose uptake experiments. The intracellular activities of hexokinase (EC 2.7.1.1) and catechol 1,2-oxygenase (EC 1.13.1.1) changed inversely when phenol was added during growth in the glucose medium.
D001693:Biological Transport, Active / Q000187:drug effects; D050548:Catechol 1,2-Dioxygenase; D002396:Catechols / Q000096:biosynthesis / Q000378:metabolism; D049308:Dioxygenases; D004790:Enzyme Induction; D005947:Glucose / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D003904:Mitosporic Fungi / Q000378:metabolism*; D010105:Oxygenases / Q000096:biosynthesis / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism* / Q000494:pharmacology
D002396:Catechols; D010636:Phenols; D010105:Oxygenases; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase; D006593:Hexokinase; D005947:Glucose