Identification of human gene products from hybrid cells: a new approach.
A method is described for detection of human polypeptides from hybrid cells, following high-resolution two-dimensional polyacrylamide gel electrophoresis of total cellular protein. 3H-labeled hybrid cell proteins are mixed with 14C-labeled parental cell proteins, electroporesis is carried out, and polypeptides that occur only in the 3H-labeled preparation are detected by double-label autoradiography. The possibility of developing a standard human polypeptide two-dimensional gel map, which would allow identification of electrophoretically separable components as specific proteins, is discussed.
D000818:Animals; D001345:Autoradiography; D002906:Chromosomes, Human, 6-12 and X; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006822:Hybrid Cells / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000032:analysis* / Q000235:genetics; D008722:Methods; D011506:Proteins / Q000032:analysis* / Q000235:genetics
D011506:Proteins; D007770:L-Lactate Dehydrogenase