Conversion of 5'-methylthioadenosine into S-adenosylmethionine by yeast cells.
5'-Methylthio[U-14C]adenosine was used as a culture supplement for Candida utilis. The resulting S-adenosylmethionine was hydrolyzed into its structural components. Virtually none of the label of the pentose was found in the carbohydrate part of the intracellular S-adenosylmethionine. Much of it was present in the four-carbon chain of the methionine part of the sulfonium compound. The (U-14C)-labeled adenine of 5'-methylthio[U-14C]adenosine did not contribute to the labeling of the amino acid components of the sulfonium compound.
D000241:Adenosine / Q000031:analogs & derivatives* / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D002175:Candida / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media; D003839:Deoxyadenosines; D012436:S-Adenosylmethionine / Q000378:metabolism*; D013872:Thionucleosides / Q000378:metabolism*
D000596:Amino Acids; D003470:Culture Media; D003839:Deoxyadenosines; D013872:Thionucleosides; C008500:5'-methylthioadenosine; D012436:S-Adenosylmethionine; D000241:Adenosine