title: [Daily rhythms of melatonin in pineal, serum and urine of rats (author's transl)].
abstract: The levels of melatonin in urine, serum, and pineals were determined in rats during the light/dark cycle. Previous to the radioimmunoassay, melatonin was isolated using a new ascending chromatographic method with programmed multiple development. The daily excretion of melatonin and its serum levels showed a characteristic rhythm, night samples containing several times more melatonin than those of the light period. The pineal content of melatonin of animals killed at different intervals also showed a similar day/night rhythm. The chromatographic method used gave a perfect separation of melatonin from other materials which could interfere in the assay. The mobility of material detected by RIA was identical to that of authentic melatonin chromatographed concurrently.
mesh_terms: D000818:Animals; D002855:Chromatography, Thin Layer; D002940:Circadian Rhythm; D008297:Male; D008550:Melatonin / Q000032:analysis* / Q000097:blood / Q000652:urine; D010870:Pineal Gland / Q000032:analysis / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats
chemical_list: D008550:Melatonin
vernacular_title: Ritmo diario en los niveles pineales, séricos y urinarios de melatonina en la rata.