An evaluation of certain factors which influence the in vitro migration of subpopulations of human lymphocytes.
The effect on the migration of subpopulations of human lymphocytes of variations in a number of factors known to influence granulocyte migration in the agarose plate system is described. The number of cells and the concentration of serum in the media had a profound effect on the migration of all lymphocyte subpopulations. However, the characteristics of the migration surface and various constituents of the agarose such as the type of serum or the addition of antibiotics or glutamine appeared to have little effect. Factors associated with homologous and heterologous sera which significantly affect granulocyte migration had little influence on the extent of lymphocyte migration, suggesting that there is some difference in the factors which regulate the migratory behavior of these cells.
D001769:Blood; D002634:Chemotaxis, Leukocyte; D003470:Culture Media; D005898:Glass; D006098:Granulocytes; D006801:Humans; D007958:Leukocyte Count; D009000:Monocytes; D010969:Plastics; D013997:Time Factors
D003470:Culture Media; D010969:Plastics