Human placental oxygen transfer and consumption. Dissociation by cooling or use of respiratory enzyme inhibitors.
The regulation of oxygen placental transfer is studied during the in vitro perfusion of normal postpartum human placental lobules. Oxygen consumption of the placental tissue is measured during the perfusion of the fetal placental circulation alone (single perfusions). To evaluate the transplacental diffusion of oxygen, fetal placental and uteroplacental circulations are simultaneously perfused (dual perfusions). The results obtained at various temperatures (37 and 4 degrees C) and after use of respiratory enzyme inhibitors (fluorodinitrobenzne and oligomycin) prove that oxygen consumption is related to trophoblast physiological activity, while oxygen transfer relies on a simple diffusion mechanism.
D001158:Arteries; D002404:Catheterization; D003080:Cold Temperature; D003864:Depression, Chemical; D004058:Diffusion; D004139:Dinitrofluorobenzene / Q000494:pharmacology; D005260:Female; D006801:Humans; D008431:Maternal-Fetal Exchange; D009840:Oligomycins / Q000494:pharmacology; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption / Q000187:drug effects; D010477:Perfusion / Q000379:methods; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D014599:Uterus / Q000098:blood supply
D009840:Oligomycins; D004139:Dinitrofluorobenzene; D010100:Oxygen