Simultaneous determination and pharmacokinetics of midazolam and its hydroxymetabolites in plasma and urine of man and dog by means of high-performance liquid chromatography.
A HPLC (high performance liquid chromatography) method for the determination of 8-chloro-6-(2-fluorophenyl)-1-methyl-4H-imidazo[1,5-a] [1,4]benzodiazepine (midazolam, Ro 21-3981, Dormicum) and its hydroxy metabolites has been developed. The half-life of elimination of midazolam and the glucuronides of the metabolites 1-hydroxymethyl-midazolam, 4-hydroxy-midazolam and 1-hydroxymethyl-4-hydroxy-midazolam are identical, 53 min in dogs and 90 min in man.
D000368:Aged; D000818:Animals; D001569:Benzodiazepines / Q000097:blood / Q000378:metabolism* / Q000652:urine; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D005260:Female; D006801:Humans; D006900:Hydroxylation; D008297:Male; D008874:Midazolam; D008875:Middle Aged; D013997:Time Factors
D001569:Benzodiazepines; D008874:Midazolam