The analysis of malignancy by cell fusion.
The lecture reviews some aspects of the work on the analysis of malignancy that have been, and are now being, pursured in the Dunn School. A brief outline of the early experiments that first demonstrated that the malignancy of mouse tumor cells can be suppressed by the fusion with normal cells is given, and then two areas of current interest in the laboratory are described. The first is an attempt to analyze the clinically important property of tumors to metastasize and the second is the work on the isolation and identification of an abnormal membrane glycoprotein present in tumor cells. In addition the value of cell fusion methods as a general test of hypotheses of malignancy is emphasized.
D000818:Animals; D001345:Autoradiography; D002459:Cell Fusion; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003208:Concanavalin A / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D006822:Hybrid Cells; D037102:Lectins / Q000378:metabolism; D008565:Membrane Proteins / Q000302:isolation & purification; D051379:Mice; D009362:Neoplasm Metastasis / Q000209:etiology; D009363:Neoplasm Proteins / Q000302:isolation & purification*; D009374:Neoplasms, Experimental / Q000032:analysis / Q000209:etiology* / Q000473:pathology
D006023:Glycoproteins; D037102:Lectins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D003208:Concanavalin A; D005947:Glucose