Inhibition of capping of immunoglobulin and concanavalin A receptors by cis-dichlorodiammine-platinum-(II) in mouse spleen cells.
Effects of cis-dichlorodiammineplatinum-(II) (cis-DDP), an anticancer agent, on capping of membrane receptors were investigated in mouse spleen cells in vitro, using fluorescein labeled ligands. Cap formations of surface immunoglobulin and of concanavalin A receptors were significantly reduced by cis-DDP in relation to dose, while cell viability remained unchanged. The binding of the ligands to specific receptors was not affected by cis-DDP. The results suggested that cis-DDP inhibited cap formation by blocking membrane movement of the ligand-receptor complexes.
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D007136:Immunoglobulins; D007152:Immunologic Capping / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D011952:Receptors, Concanavalin A / Q000187:drug effects; D011971:Receptors, Immunologic / Q000187:drug effects*; D013154:Spleen / Q000187:drug effects
D007136:Immunoglobulins; D011952:Receptors, Concanavalin A; D011971:Receptors, Immunologic; D002945:Cisplatin