Visualization of Z sequences in form V of pBR322 by immuno-electron microscopy.
Form V DNA has been prepared from pBR322 DNA by annealing covalently closed complementary single strands. Specific rabbit antibodies to Z-DNA were shown by radioimmunoassay and electron microscopy to react with form V DNA of pBR322. The bound antibodies were visualized either directly (on synthetic polynucleotides in Z-form), or after reaction with goat anti-rabbit immunoglobulin labeled with ferritin (on form V DNA).
D000936:Antigen-Antibody Complex; D001483:Base Sequence; D004247:DNA; D007106:Immune Sera; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D010957:Plasmids; D011089:Polydeoxyribonucleotides; D011863:Radioimmunoassay
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D011089:Polydeoxyribonucleotides; C019530:poly(dC-dG); D004247:DNA