A spectrofluorometric investigation, using 1-anilino-naphthalene-8-sulphonate, of the interaction between washed bovine spermatozoa and seminal plasma or egg-yolk lipoprotein.
The dye 1-anilino-naphthalene-8-sulphonate (ANS) bound at 94.5 +/- 2.7 pmol per 10(6) spermatozoa (KD = 1.83 +/- 0.13 X 10(-5) M) and 7.30 +/- 0.17 pmol per mg seminal plasma protein (KD = 2.19 +/- 0.06 X 10(-5) M). Equilibration of excess ANS with a mixture of washed spermatozoa and seminal plasma resulted in a significant increase in fluorescence over that calculated by summation of the individual levels (P less than 0.05). A less pronounced but significant increase was seen when an egg-yolk lipoprotein, previously shown to have cryoprotective properties, was similarly added to washed spermatozoa (P less than 0.05). This increase in fluorescence was not reduced by washing spermatozoa--lipoprotein mixtures, suggesting that an interaction between the lipoprotein and the cell membrane had occurred.
D000817:Anilino Naphthalenesulfonates / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D003451:Cryoprotective Agents / Q000378:metabolism*; D004530:Egg Yolk; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism*; D008074:Lipoproteins / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D012661:Semen / Q000378:metabolism*; D012662:Semen Preservation; D013050:Spectrometry, Fluorescence; D013094:Spermatozoa / Q000378:metabolism*
D000817:Anilino Naphthalenesulfonates; D003451:Cryoprotective Agents; D005456:Fluorescent Dyes; D008074:Lipoproteins; C027132:1-anilino-8-naphthalenesulfonate