Quantitation of sulfhydryl groups on erythrocytes in polycythemia vera.
The number of sulfhydryl groups on the surface of intact erythrocytes from patients with polycythemia vera is significantly less (34%) than those from control subjects. Sulfhydryl groups were measured indirectly by reversibly blocking -SH groups with the thiol reagent, 6,6'-dithiodinicotinic acid, which forms stable mixed disulfides on the surface of erythrocytes. Glutathione was used to break the disulfides and release thione into the supernatant. Thione was then quantitated spectrophotometrically.
D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D011087:Polycythemia Vera / Q000097:blood*; D013438:Sulfhydryl Compounds / Q000097:blood*
D013438:Sulfhydryl Compounds