Refection in rats fed on a sucrose-based, riboflavin-deficient diet.
1. Refection, resulting in an increased supply of riboflavin to riboflavin-deficient rats through coprophagy, was demonstrated on a sucrose-based diet when sensitive biochemical tests of riboflavin status were employed: these included measurements of NAD(P)H2:glutathione oxidoreductase (EC 1.6.4.2); succinate:(acceptor) oxidoreductase (EC 1.3.99.1) and NADH:(acceptor) oxidoreductase (EC 1.6.99.3). 2. The use of tail-cups to eliminate coprophagy, and hence refection, resulted in a more rapid and reproducible progress into severe deficiency. 3. The occurrence of refection on a sucrose-based diet may account for hitherto unexplained differences between previous publications on the biochemical effects of riboflavin deficiency.
D000818:Animals; D003303:Coprophagia / Q000378:metabolism; D004040:Dietary Carbohydrates / Q000378:metabolism*; D005980:Glutathione Reductase / Q000378:metabolism; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D008099:Liver / Q000201:enzymology; D008297:Male; D009245:NADH Dehydrogenase / Q000378:metabolism; D051381:Rats; D012257:Riboflavin Deficiency / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism*
D004040:Dietary Carbohydrates; D013395:Sucrose; D013385:Succinate Dehydrogenase; D009245:NADH Dehydrogenase; D005980:Glutathione Reductase