DNA polymerase of a basidiomycete fungus, Coprinus cinereus.
DNA polymerase activity was studied in Coprinus cinereus, a basidiomycete fungus. Only one from of the enzyme could be demonstrated, whether by affinity or ion-exchange chromatography; this enzyme had a molecular weight of 185000 on Sephadex G-200, and was inhibited by mercaptoethanol. Coprinus, a representative of the most advanced type of the filamentous fungi, resembles other eukaryotic micro-organisms in its lack of a mammalian beta-type DNA polymerase. The properties of the polymerase are compared with those of two other fungi, and found to resemble most closely the yeast polymerase A in Mg2+ requirements and template preference.
D000363:Agaricales / Q000201:enzymology*; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D003302:Coprinus / Q000201:enzymology*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D008970:Molecular Weight; D019384:Nucleic Acid Synthesis Inhibitors; D013698:Templates, Genetic
D019384:Nucleic Acid Synthesis Inhibitors; D004259:DNA-Directed DNA Polymerase