title: Abnormal embryonic development induced by antiserum: a rapid method to isolate the responsible galactoprotein antigens.
abstract: It has been well established that heterologous antiserum against whole rat kidney homogenate when injected into pregnant rats during the organogenetic period may induce abnormal embryonic development. A one-step rapid method has been developed to isolate the responsible rat renal antigens by lectin-affinity chromatography using soy bean agglutinin (SBA) or ricinus communis agglutinin (RCA120). The purified antigens appeared to be galactoproteins with terminal nonreducing D-galactose and/or N-acetyl-D-galactosamine. Rabbit antisera produced against the galactoproteins were potent teratogenic agents.
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex; D000941:Antigens / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004622:Embryo, Mammalian / Q000276:immunology / Q000502:physiology*; D005260:Female; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification*; D007106:Immune Sera; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000936:Antigen-Antibody Complex; D000941:Antigens; D006023:Glycoproteins; D007106:Immune Sera; C017336:galactoproteins