An optically clear hypercholesterolemic hypertriglyceridemic quality-control material prepared from animal lipid sources.
A hyperlipidemic control serum can be simple prepared from animal lipid sources. Beta- and pre-beta-lipoproteins containing cholesterol and triglyceride are removed from porcine serum by treatment with dextran sulfate and calcium ions. A triglyceride-rich fraction containing only trace amounts of cholesterol is isolated from chicken egg-yolks. The two fractions are then combined in 40 mmol/L sodium bicarbonate to give the desired values for cholesterol and triglyceride. The preparation is stabilized against surface denaturation during long-term storage at 5 degrees C perhaps for as long as two years, by adding 0.25 g of Triton X-100 surfactant per liter, and against an accidental exposure to short-term freezing by adding 10 g of sucrose per liter. We used this solution as a diluent to reconstitute lyophilized bovine serum. The resulting product, having been prepared from only animal sources, is free of hepatitis-associated constituents, and is remarkably clear, homogeneous, and stable. Results obtained with it are precise.
D000818:Animals; D002417:Cattle / Q000097:blood; D002645:Chickens; D002784:Cholesterol / Q000302:isolation & purification; D004530:Egg Yolk / Q000032:analysis; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood*; D006949:Hyperlipidemias / Q000097:blood*; D008074:Lipoproteins / Q000302:isolation & purification; D011309:Preservation, Biological; D011786:Quality Control; D012015:Reference Standards; D013552:Swine / Q000097:blood; D014280:Triglycerides / Q000302:isolation & purification
D008074:Lipoproteins; D014280:Triglycerides; D002784:Cholesterol