title: Investigations into falsely elevated somatic cell counts of bulked herd milk.
abstract: Bulk milk cell counts obtained by a Coulter Milk Cell Counter were shown to be falsely elevated because of the formation of non-cellular particles (NCP). The NCP, which were detected by the use of a particle size analyser, were removed if the milk samples were heated to 55 degrees C for 15 min before the addition of formalin. A survey of bulk milk supplies in New South Wales showed that NCP occurred in milk from 2% of farms, the counts being falsely elevated by about 200 000 cells/ml. Surveys of milking equipment of farms where the bulk milk had shown this phenomenon demonstrated a relationship between the use of centrifugal milk pumps and the presence of NCP with a much lower prevalence in herds where spit chamber releasers were used. These particles were not air bubbles since air was removed from the milk by heating to 60 degrees C for 15 min, vacuum at 65 mmHg for 15 min or 0.5% v/v octan-2-ol; only the heat treatment removed the NCP.
mesh_terms: D000818:Animals; D001315:Australia; D002417:Cattle / Q000033:anatomy & histology* / Q000502:physiology; D002452:Cell Count / Q000662:veterinary; D003612:Dairying / Q000295:instrumentation; D005260:Female; D006358:Hot Temperature; D007774:Lactation; D008892:Milk / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy