[Multiparameter analysis of uterine cancer cells using flow-cytometry-computer].
In recent progress, Laser-based flow cytometric technique makes it possible to do quantitative determination of nuclear DNA-RNA content and proteins on monodisperse cell population simultaneously. Gynecologic cytology specimens that derived from the cervix and the endometrium were stained with a combination of propidium iodide for nuclear DNA-RNA and fluorescein isothiocyanate for protein in cells, then analyzed using a flow cytometry system with computer. These results included assessment of the presence or absence of malignant cells. They also demonstrated the dispersion contour of the normal squamous cell population, and inflammatory responding cell population. Measurements of clinical specimens were resulted with a 9.6% false negative rate and a 27.0% false positive rate (80.6% in accuracy rate) by comparison with routine visual cytologic evaluation of the same samples.
D003201:Computers; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D007834:Lasers; D009363:Neoplasm Proteins / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D014594:Uterine Neoplasms / Q000175:diagnosis* / Q000473:pathology
D004273:DNA, Neoplasm; D005456:Fluorescent Dyes; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm