Mapping a marsupial X chromosome using kangaroo-mouse somatic cell hybrids.
A series of marsupial-eutherian somatic cell hybrids was produced by fusion between lymphocytes from the red kangaroo (Macropus rufus) and HPRT-deficient mouse cells. The hybrids lost marsupial chromosomes and could therefore be used to map marsupial genes. Several of the hybrids contained a complete red kangaroo X chromosome and expressed the kangaroo form of the enzymes HPRT, G6PD, and PGKA. A number of HPRT-deficient revertant cell lines were derived from the hybrids. These possessed a variety of partially deleted X chromosomes. With these cell lines, it has been possible to establish the X-linkage of the genes for HPRT, G6PD, and PGKA in this marsupial and to localize these three genes to the terminal portion of the euchromatic arm of the red kangaroo X chromosome.
D000818:Animals; D002460:Cell Line; D002871:Chromosome Banding; D002874:Chromosome Mapping; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics; D006822:Hybrid Cells; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D007614:Macropodidae / Q000235:genetics* / Q000378:metabolism; D008394:Marsupialia / Q000235:genetics*; D051379:Mice; D010735:Phosphoglycerate Kinase / Q000235:genetics; D012730:Sex Chromosomes; D014960:X Chromosome
D005954:Glucosephosphate Dehydrogenase; D007041:Hypoxanthine Phosphoribosyltransferase; D010735:Phosphoglycerate Kinase