Hexavalent chromium and ascorbic acid interaction on proliferation of the human cell line NHIK3025.
When cells from the human cell line NHIK3025 were cultured for 4 days, with hexavalent chromium (K2Cr2O7) at a concentration of 8 . 10(-7)M, the relative cell number was reduced to 38%. This effect of hexavalent chromium was abolished if ascorbic acid and chromate were added simultaneously to the incubation medium, but not if ascorbic acid was added 24 h prior to chromate addition. Dehydroascorbic acid was not able to reduce the effect of K2Cr2O7 either when added simultaneously or when added 24 h prior to chromate exposure. Therefore, the cells could only be protected against the toxic effect of hexavalent chromium when ascorbic acid was added at the same time as K2Cr2O7.
D001205:Ascorbic Acid / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002840:Chromates / Q000633:toxicity; D002857:Chromium / Q000378:metabolism / Q000633:toxicity*; D003683:Dehydroascorbic Acid / Q000494:pharmacology; D005260:Female; D006801:Humans; D017680:Potassium Compounds; D002583:Uterine Cervical Neoplasms
D002840:Chromates; D017680:Potassium Compounds; D002857:Chromium; C027373:potassium chromate(VI); D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid