Immunosuppressive effect of a trichothecene mycotoxin, Fusarenon-X in mice.
The in vitro treatment with Fusarenon-X, a mycotoxin produced by Fusarium nivale Fn 2B, depressed the mitogenic responses of mouse lymphocytes to the T-cell mitogens, phytohaemagglutinin (PHA) and concanavalin A (Con A), but to a lesser extent to B-cell mitogen, a bacterial lypopolysaccharide (LPS). The in vitro treatment of mice with Fusarenon-X also decreased the responsiveness of splenic lymphocytes to the T-cell mitogen, Con A. Administration of Fusarenon-X to BALB/c mice before immunization significantly reduced anti-DNP IgE and IgG1 responses, while the treatment of mice after immunization was less effective. The number of spleen cells of the treated mice was increased with a reduction of T lymphocytes and an increase of granulocytic elements. In vitro antibody responses of spleen cells from Fusarenon-X treated mice by pokeweed mitogen (PWM) or LPS were also suppressed. Reconstruction experiments using sIg positive and negative spleen cells from normal and Fusarenon-X treated mice showed that this inhibitory effect resided in the sIg negative cell population.
D000818:Animals; D000921:Antibody-Producing Cells / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D007073:Immunoglobulin E / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009183:Mycotoxins / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011947:Receptors, Antigen, B-Cell; D012717:Sesquiterpenes / Q000494:pharmacology*; D014255:Trichothecenes / Q000494:pharmacology*
D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; D008070:Lipopolysaccharides; D009183:Mycotoxins; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D011947:Receptors, Antigen, B-Cell; D012717:Sesquiterpenes; D014255:Trichothecenes; D003208:Concanavalin A; C002469:fusarenon-X; D007073:Immunoglobulin E