Endogenous GABA release from slices of rat cerebral cortex and hippocampus in vitro.
Using a rapid, simple and sensitive radioreceptor assay, a Ca2+-dependent K+-evoked release of endogenous GABA was demonstrated from rat cortical and hippocampal slices in vitro. This evoked-release of endogenous GABA was similar to that of [3H]GABA release (in its Ca2+ dependency) but differed from the latter in having a higher signal to noise level. Neither 5-HT nor a stable enkephalin analogue had any effect on endogenous GABA release from hippocampus slices.
D000818:Animals; D002118:Calcium / Q000502:physiology; D002540:Cerebral Cortex / Q000378:metabolism*; D004745:Enkephalins / Q000494:pharmacology; D006624:Hippocampus / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D004745:Enkephalins; D012701:Serotonin; D005680:gamma-Aminobutyric Acid; C027970:enkephalin, AlaNH2(5)-; D011188:Potassium; D002118:Calcium