title: H-Y antigen negative germ cells in gonadal sex organization in vitro: a morphological study.
abstract: Dissociation-reorganization experiments were done with gonadal cells of newborn rats. Rotation cultures consisted of mixtures of somatic and germ cells of opposite sex. Somatic cells, ovarian or testicular, determined a female or male type respectively, of gonadal histomorphic organization. Germ cells did not affect the type of organization of somatic cells. Accordingly, suspensions containing somatic cells of one sex together with germ cells of both sexes, reorganized in rotation culture, into either a) follicles containing XX or XY germ cells, or b) tubules containing XX or XY or both types of germ cells. These results give morphological evidence for heterosexual germ-somatic cells interactions. Based on morphological and H-Y antigen studies, failure of germ cells to bind and express H-Y antigen is considered as a possible factor for this failure of germ cells to affect gonadal sex.
mesh_terms: D002478:Cells, Cultured / Q000166:cytology*; D005260:Female; D006182:H-Y Antigen / Q000276:immunology*; D008297:Male; D009024:Morphogenesis; D010053:Ovary / Q000166:cytology*; D010063:Ovum / Q000166:cytology / Q000276:immunology; D013094:Spermatozoa / Q000166:cytology / Q000276:immunology; D013737:Testis / Q000166:cytology*
chemical_list: D006182:H-Y Antigen