Rapid synthesis of oligodeoxyribonucleotides. VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide support.
A new kieselguhr-polydimethylacrylamide support has been used in a continuous flow, column system for solid phase synthesis of oligodeoxyribonucleotides by a phosphotriester procedure. Using only protected mononucleotides a 14-mer, 20-mer and 27-mer were assembled in high repetitive yield using a simple manually operated, bench top apparatus.
D000178:Acrylamides; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003854:Deoxyribonucleotides; D007692:Diatomaceous Earth; D007202:Indicators and Reagents; D008768:Methylmethacrylates; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009841:Oligonucleotides / Q000138:chemical synthesis*
D000178:Acrylamides; D003854:Deoxyribonucleotides; D007202:Indicators and Reagents; D008768:Methylmethacrylates; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D007692:Diatomaceous Earth