Microsomal lipids as targets for halothane metabolites.
The lipid binding sites of halothane metabolites were examined in an in vitro microsomal system. Using halothane-1-14C, binding was detected to all phospholipid classes in rat liver microsomes, however, phosphatidyl ethanolamine bound halothane with a higher specific activity than phosphatidyl choline. Phosphatidyl choline contained the greatest amount of label, and the major binding site in purified phosphatidyl choline was found to be associated with the fatty acid portion of the molecule. Neutral lipid binding also occurred in this system.
D000818:Animals; D001665:Binding Sites; D006221:Halothane / Q000378:metabolism*; D050356:Lipid Metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D010743:Phospholipids; D006221:Halothane