Influence of angiotensins (I, II & III) on prostaglandin production by minced rat renal medulla.
Minced rat renal medulla was incubated for 30 min at 37 degrees C in the presence of angiotensin I, II or III (100 ng/ml) to determine the existence of a direct stimulating effect on prostaglandin (PG) production. PGE2, PGF2 alpha, 6-keto PGF1 alpha and Thromboxane B2 (TXB2) were determined by radioimmunoassay. For analysis of data variance, the results were separated according to whether the net output of PGE2 was above or below 1.5 ng PGE2 equivalent/mg tissue/30 min. Under low-output conditions, angiotensin I, II or III stimulated PGE2 production significantly (p less than 0.02) and tended to augment PGF2 alpha production, while under high-output conditions no effect on PGE2 or PGF2 alpha production was observed. Under either output condition, angiotensin I, II or III had no effect on 6-keto PGF1 alpha and TXB2.
D000527:Alprostadil; D000803:Angiotensin I / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology; D000805:Angiotensin III / Q000494:pharmacology; D000809:Angiotensins / Q000494:pharmacology*; D000818:Animals; D066298:In Vitro Techniques; D007679:Kidney Medulla / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011453:Prostaglandins / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013929:Thromboxane B2 / Q000096:biosynthesis
D000809:Angiotensins; D011453:Prostaglandins; D011458:Prostaglandins E; D000804:Angiotensin II; D000805:Angiotensin III; D013929:Thromboxane B2; D000803:Angiotensin I; D000527:Alprostadil