The effect of ethanol and/or acetaldehyde on the incorporation of U14C-glucose into human umbilical artery lipids.
The effect of ethanol, acetaldehyde and a combination of ethanol and acetaldehyde added in vitro was determined on their abilities to alter conversion of glucose to lipid. When human umbilical arteries were perfused with acetaldehyde at a 0.5% concentration, a significant reduction (p less than 0.05) of 14C incorporation into lipid was observed. This reduction was found to be the result of depressed incorporation of glucose into phospholipid (PL) and triacylglycerides (TG). Acetaldehyde at a concentration of 0.25% appeared to depress incorporation; however, this was not significant. Acetaldehyde plus ethanol at final equal concentrations of 0.25%, 0.5% and 1.0% had no effect on incorporation of U14 C-glucose into lipid. Ethanol at concentrations of 0.5%, 0.25% and 0.125% had no effect on the incorporation of U14 C-glucose into lipid. The study suggests that acetaldehyde can depress the conversion of glucose into umbilical artery lipids. However, acetaldehyde in the presence of equal concentrations of ethanol does not exhibit this ability to depress conversion of glucose to lipids suggesting some cellular counter effect of these two agents.
D000079:Acetaldehyde / Q000494:pharmacology*; D001158:Arteries / Q000187:drug effects* / Q000378:metabolism; D002250:Carbon Radioisotopes; D002784:Cholesterol / Q000096:biosynthesis; D004075:Diglycerides / Q000096:biosynthesis; D000431:Ethanol / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008055:Lipids / Q000096:biosynthesis*; D010743:Phospholipids / Q000096:biosynthesis; D014280:Triglycerides / Q000096:biosynthesis; D014469:Umbilical Arteries
D002250:Carbon Radioisotopes; D004075:Diglycerides; D005230:Fatty Acids, Nonesterified; D008055:Lipids; D010743:Phospholipids; D014280:Triglycerides; D000431:Ethanol; D002784:Cholesterol; D000079:Acetaldehyde; D005947:Glucose