Glucocorticoid binding and cytolethal responsiveness of hairy-cell and chronic lymphocytic leukaemia.
The glucocorticoid binding properties and cytolethal responsiveness of leukaemic cells were studied in vitro in seven patients with hairy-cell leukaemia (HCL) and five with chronic lymphocytic leukaemia (CLL). Substantial levels of glucocorticoid binding were detected both in whole cell and cytosol preparations from all patients although the level of binding by HCL cells always exceeded that of CLL cells (P less than 0.05). In both leukaemic cell types the uptake and binding of prednisolone in vitro was significantly greater than that of dexamethasone (P less than 0.05). CLL cells showed a variable dose-related cytolethal response to methylprednisolone sodium succinate (MPSS) treatment in vitro although cytolytic effects were not marked in the usual pharmacological dose range (10(-5)-10(-6)M). Treatment of CLL patients with conventional doses of prednisone for extended periods or high intravenous infusions of MPSS over shorter periods had no consistent effect on the in-vitro level of steroid binding or the cytolethal responsiveness of CLL cells to glucocorticoid treatment. Although HCL cells proved highly resistant to the cytolethal effects of MPSS in vitro, the substantial binding of glucocorticoids by leukaemic cells from all HCL patients indicates the potential value of steroid therapy in this disease should be explored further.
D000284:Administration, Oral; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000378:metabolism; D003907:Dexamethasone / Q000627:therapeutic use; D006801:Humans; D007275:Injections, Intravenous; D007943:Leukemia, Hairy Cell / Q000188:drug therapy* / Q000378:metabolism; D007945:Leukemia, Lymphoid / Q000188:drug therapy* / Q000378:metabolism; D011239:Prednisolone / Q000008:administration & dosage / Q000627:therapeutic use; D011241:Prednisone / Q000008:administration & dosage / Q000627:therapeutic use; D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D003907:Dexamethasone; D011239:Prednisolone; D011241:Prednisone