Separation of subunits of pike eel gonadotropin by hydrophobic interaction chromatography.
Subunits of pike eel gonadotropin were dissociated in propionic acid and separated by hydrophobic interaction chromatography on phenyl-Sepharose CL-4B with a high yield (79%). Isolated subunits were homogeneous, as tested by sodium dodecyl sulphate disc electrophoresis and by N-terminal analysis. The biological activities of two subunits, S-I and S-II, were 0% and 2.8% of native molecule, respectively, as assayed by stimulation of androgen production on carp testes. Reassociated molecules restored about 75% activity.
D000818:Animals; D002845:Chromatography / Q000379:methods; D004524:Eels; D005399:Fishes; D006062:Gonadotropins / Q000032:analysis* / Q000494:pharmacology; D008297:Male; D013737:Testis / Q000187:drug effects / Q000378:metabolism
D006062:Gonadotropins