Influence of diphenylhydantoin on isotope release and bone enzymes in vitro.
The acute effect of diphenylhydantoin isotope release in an in vitro system using mouse calvaries was studied. A depressive effect on bone resorption was found involving radioactive calcium as well as tritiated proline. Bone resorption and formation were further investigated by semiquantitative histochemistry. Depressed activity of both alkaline and acid phosphatase was found, indicating a direct inhibitory effect of diphenylhydantoin on bone turnover.
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001862:Bone Resorption / Q000187:drug effects*; D001842:Bone and Bones / Q000187:drug effects / Q000201:enzymology*; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D066298:In Vitro Techniques; D051379:Mice; D010672:Phenytoin / Q000494:pharmacology*; D011392:Proline / Q000378:metabolism
D002132:Calcium Radioisotopes; D010672:Phenytoin; D011392:Proline; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D002118:Calcium