title: Dual column chromatographic method for determination of N-nitrosothiazolidine in fried bacon.
abstract: A dual column chromatographic procedure is described that permits the determination of N-nitrosothiazolidine (NTHZ), a newly identified nitrosamine, and N-nitrosopyrrolidine (NPYR), the principal volatile nitrosamine, in the same fried bacon sample. The procedure used an acid-Celite, Celite, sodium sulfate column followed by a partially deactivated basic alumina column to isolate NTHZ from bacon for subsequent quantitation by gas chromatography with a thermal energy analyzer (GC-TEA). A limited intralaboratory study gave standard deviations for repeatability and reproducibility of 1.20 and 1.55 ppb, respectively. The recovery of N-nitrosothiomorpholine, the internal standard added at the 10 ppb level, was 93.3 +/- 6.03%. The method is also free from artifactual nitrosamine formation.
mesh_terms: D000818:Animals; D002845:Chromatography / Q000379:methods; D002849:Chromatography, Gas; D008460:Meat / Q000032:analysis*; D009602:Nitrosamines / Q000032:analysis*; D013552:Swine
chemical_list: D009602:Nitrosamines