Quantitative separation of estrogens, androgens and progestogens using reverse phase high pressure liquid chromatography.
Dual UV wavelength scanning at 206 and 254 nm was used to develop a sensitive, separation and quantitation procedure for estrone, estradiol-17 beta, testosterone, 4-androstene-3,17,-dione, progesterone and 17-hydroxyprogesterone using reverse phase high pressure liquid chromatography. The difference in UV absorption of the estrogens from the androgens and progestogens allows for correction of the co-elution of testosterone with estradiol-17 beta and 4-androsten-3,17,-dione with estrone. Incorporation of an isocratic step-gradient provides improved separation while maintaining shortened elution times for the less polar steroids.
D019326:17-alpha-Hydroxyprogesterone; D000728:Androgens / Q000097:blood*; D000735:Androstenedione / Q000097:blood; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004958:Estradiol / Q000097:blood; D004967:Estrogens / Q000097:blood*; D004970:Estrone / Q000097:blood; D006908:Hydroxyprogesterones / Q000097:blood; D011374:Progesterone / Q000097:blood; D011372:Progestins / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013739:Testosterone / Q000097:blood
D000728:Androgens; D004967:Estrogens; D006908:Hydroxyprogesterones; D011372:Progestins; D004970:Estrone; D013739:Testosterone; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D019326:17-alpha-Hydroxyprogesterone