Isolation of six monoclonal alloantibodies against rat histocompatibility antigens: clonal competition.
We describe the isolation of six clones and some variant derivatives of rat x mouse hybrid myelomas secreting alloantibody against antigens of the rat major histocompatibility complex. Very large numbers of active hybrids were obtained but many were lost early in the post-fusion period; evidence is presented for rapid selective processes operating in uncloned complex hybrid cultures. The results suggest that the secretion of specific immunoglobulin chains is as stable a function in rat x mouse hybrid myelomas as in mouse x mouse hybrid myelomas.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D002999:Clone Cells; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006461:Hemolysis; D006822:Hybrid Cells / Q000276:immunology; D007518:Isoantibodies / Q000302:isolation & purification*; D008285:Major Histocompatibility Complex; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D007518:Isoantibodies