Interaction of the plasma membrane of mouse, rat and hamster oocytes with human spermatozoa in vitro.
The study of the interaction of "zona-free" mouse, rat and hamster eggs with human spermatozoa showed clearly the species specificity of the plasma membrane in mice and rats where sperm penetration into the cytoplasm has never been observed. On the other hand, 66.1--77.0% of hamster eggs were penetrated and 21.9-57.5% of these eggs showed polyspermy. These results were not affected by any of the three proteolytic enzymes used for dissolution of the zona pellucida. Penetration activity of human spermatozoa expressed as percentage of penetrated zona-free hamster eggs decreased after 12 h of preincubation to 12.4% compared to 72.6% in spermatozoa preincubated for 6 h.
D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D002918:Chymotrypsin / Q000494:pharmacology; D006224:Cricetinae; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D051379:Mice; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010063:Ovum / Q000502:physiology*; D011402:Pronase / Q000494:pharmacology; D051381:Rats; D013045:Species Specificity; D013075:Sperm Capacitation; D013094:Spermatozoa / Q000502:physiology*; D014357:Trypsin / Q000494:pharmacology; D015044:Zona Pellucida / Q000187:drug effects
D002918:Chymotrypsin; D014357:Trypsin; D011402:Pronase