Identification of a brain theta-positive, secretory cell from hematopoietic tissues.
We have characterized a population of large, brain-theta antigen-positive, secretory cells that occur in high frequency among murine bone marrow (approximately 6%), spleen (approximately 0.5%), and fetal liver (approximately 5%) cell populations. They do not occur in significant numbers among lymph node, thymus, and peritoneal exudate cells. These cells are Ly1, Ly2, and Thy1.2 negative, adherent to nylon wool, Sephadex G-10, and plastic, but peroxidase and Sudan black negative. They are radiation, cyclophosphamide, and cortisone resistant. These cells secrete a product of 30,000 to 40,000 m.w. of unknown function. Production of antisera specific for this molecule has facilitated identification of the cell by using a reverse hemolytic plaque-forming cell assay. Possible functions of this cell and its product are discussed.
D000818:Animals; D000921:Antibody-Producing Cells / Q000166:cytology / Q000276:immunology; D000941:Antigens; D001853:Bone Marrow / Q000276:immunology; D001921:Brain / Q000276:immunology*; D002469:Cell Separation; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000378:metabolism*; D007106:Immune Sera / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits
D000941:Antigens; D007106:Immune Sera