[Carbohydrate content of lipoproteins after sequential precipitation with phosphotungstate magnesium chloride].
A method is described for rapid determination of protein-bound carbohydrates in human lipoproteins. The method is based on a successive precipitation of lipoproteins with sodium phosphotungstate and magnesium chloride. After purification of the separated lipoproteins by a twofold reprecipitation, a colorimetric determination of hexoses, hexosamines and N-acetylneuraminic acid (sialic acid) is performed. The coefficients of variation in the series are between 3.2% and 7.3%. The results for lipoprotein-bound carbohydrates after precipitation are compared with the corresponding values after separation by ultracentrifugation. The concentrations of hexoses, hexosamines and N-acetylneuraminic acid in the 3 lipoprotein classes VLDL, LDL and HDL of control persons are reported.
D002241:Carbohydrates / Q000032:analysis*; D011232:Chemical Precipitation; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D006801:Humans; D008074:Lipoproteins / Q000032:analysis*; D008274:Magnesium; D015636:Magnesium Chloride; D010772:Phosphotungstic Acid; D012794:Sialic Acids / Q000032:analysis; D014461:Ultracentrifugation
D002241:Carbohydrates; D006595:Hexosamines; D006601:Hexoses; D008074:Lipoproteins; D012794:Sialic Acids; D015636:Magnesium Chloride; D010772:Phosphotungstic Acid; D008274:Magnesium
Kohlenhydratgehalt von Lipoproteinen nach sequentieller Präzipitation mittels Phosphorwolframat-Magnesiumchlorid.