Biosynthesis of xyloglucan in suspension-cultured soybean cells. An assay method for xyloglucan xylosyltransferase and attempted synthesis of xyloglucan from UDP-D-xylose.
Hydrolysis of the total non-cellulosic 14C-polysaccharides from soybean cell wall with carbohydrate hydrolases of an Aspergillus oryzae enzyme preparation yielded 14C-monosaccharides and [14C]isoprimeverose (6-O-alpha-D-xylopyranosyl-D-glucopyranose) in which all of the xylosyl residues of the xyloglucan were recovered. Based on this finding, an assay method for the activity of xyloglucan xylosyltransferase was developed. When UDP-[14C]xylose was incubated with a particulate enzyme fraction from soybean cells, radioactive polymers were synthesized. On digestion with the A. oryzae enzyme preparation, the product gave 14C-monosaccharides and [14C]isoprimeverose. This result suggests that the activity of xyloglucan xylosyltransferase can be distinguished from other polysaccharide synthase activities.
D001236:Aspergillus oryzae / Q000201:enzymology; D002250:Carbon Radioisotopes; D005936:Glucans; D006026:Glycoside Hydrolases / Q000378:metabolism; D010430:Pentosyltransferases / Q000378:metabolism*; D010944:Plants / Q000201:enzymology*; D011134:Polysaccharides / Q000096:biosynthesis*; D013025:Glycine max; D014539:Uridine Diphosphate Sugars / Q000378:metabolism*; D014540:Uridine Diphosphate Xylose / Q000378:metabolism*; D014990:Xylans
D002250:Carbon Radioisotopes; D005936:Glucans; D011134:Polysaccharides; D014539:Uridine Diphosphate Sugars; D014990:Xylans; D014540:Uridine Diphosphate Xylose; C029353:xyloglucan; D010430:Pentosyltransferases; C029368:xyloglucan xylosyltransferase; D006026:Glycoside Hydrolases