Developmental regulation of protein synthesis during Polysphondylium pallidum cyst germination: a two-dimensional gel electrophoresis study.
Microcyst germination in Polysphondylium pallidum can be used as a model for studying gene expression because temporally regulated modulations in protein synthesis occur in this developmental pathway. Germinating cysts were labeled with [35S]methionine for half-hourly periods during the synchronous germination sequence, and the proteins labeled in each period were resolved by two-dimensional polyacrylamide gel electrophoresis. Three major classes of proteins observed were distinguished by the time of onset and duration of their synthesis: (a) proteins made throughout germination; (b) proteins synthesized only during a portion of the germination pathway; and (c) polypeptides whose synthesis started at 1 or 1.5 h and then continued throughout germination.
D002453:Cell Cycle; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000096:biosynthesis*; D009235:Myxomycetes / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D013997:Time Factors
D005656:Fungal Proteins