Cryopreservation of buffalo semen.
Twenty-one split semen samples from five bulls were diluted in five diluents. The diluents comprised lactose-egg yolk-glycerol, fructose-egg yolk-glycerol and three different combinations of lactose and fructose with equal proportions of egg yolk and glycerol. The straw-method of deep freezing was used. Frozen semen was evaluated on the basis of post thawing motility percentage, survival of spermatozoa at 37 degrees C and absolute index of survival. Statistical analysis of data revealed highly significant differences between diluents for the three characteristics tested. The diluent comprising 11 per cent lactose 56.25 ml + 6 per cent fructose 18.75 ml + egg yolk 20 ml + glycerol 5 ml achieved the best results.
D000818:Animals; D002020:Buffaloes; D004530:Egg Yolk; D005260:Female; D005615:Freezing; D005632:Fructose / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D007785:Lactose / Q000494:pharmacology; D008297:Male; D010406:Penicillins / Q000494:pharmacology; D012662:Semen Preservation / Q000379:methods; D013081:Sperm Motility / Q000187:drug effects; D013307:Streptomycin / Q000494:pharmacology
D010406:Penicillins; D005632:Fructose; D007785:Lactose; D005990:Glycerol; D013307:Streptomycin