Quantitative x-ray microanalysis of spleen lysosomes after acid phosphatase reaction.
A quantitative X-ray microanalytical study of 80 spleen lysosomes after histochemical reaction for acid phosphatase was carried out. Lysosomal Fe concentrations showed a skewed distribution, with most lysosomes having relatively low concentrations. The concentration of Pb in the lysosomes (indicative of acid phosphatase activity) showed a close to normal distribution. Although the plot of lysosomal Pb concentrations against Fe concentrations showed a marked scattering of the points, there is a statistically highly significant correlation between accumulated metal and acid phosphatase activity as determined from the Pb concentrations. The relation between concentrations of P and those of Pb in the lysosomes points to the possibility that Pb(H2PO4)2 is the main reaction product of the histochemical acid phosphatase reaction.
D000135:Acid Phosphatase / Q000032:analysis*; D000818:Animals; D003593:Cytoplasm / Q000032:analysis; D004577:Electron Probe Microanalysis; D006651:Histocytochemistry; D007501:Iron / Q000032:analysis*; D007854:Lead / Q000032:analysis; D008247:Lysosomes / Q000032:analysis* / Q000201:enzymology; D008264:Macrophages / Q000032:analysis / Q000648:ultrastructure*; D008297:Male; D010758:Phosphorus / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000648:ultrastructure
D010758:Phosphorus; D007854:Lead; D007501:Iron; D000135:Acid Phosphatase