[Preparation of pure lines of thymus stromal fibroblasts and markers for differentiating fibroblasts and epithelia in thymus culture].
Explantation of suspensions of the cells isolated from the guinea-pig thymus by mechanical treatment leads to the formation in monolayer cultures of discrete fibroblast colonies, meanwhile explantation of the cells obtained by trypsinization results in fibroblast colonies apart from foci of the epithelium. These two types of the cells are discriminated on the basis of their fixation with antifibronectin and antifibroblast sera as well as by adhesiveness of epithelial cells and fibroblasts.
D000818:Animals; D002460:Cell Line; D004847:Epithelial Cells; D005347:Fibroblasts / Q000166:cytology*; D006168:Guinea Pigs; D013950:Thymus Gland / Q000166:cytology*
Poluchenie chistykh liniĭ stromal'nykh fibroblastov timusa i priznaki, po kotorym fibroblasty i épiteliĭ razlichaiutsia v kul'turakh timusa.