Simultaneous determination of antipyrine, 4-hydroxyantipyrine, 3-hydroxymethylantipyrine and norantipyrine in urine by gas chromatography chemical ionization mass spectrometry.
A simple, accurate and precise procedure was developed to quantify, in a single run, antipyrine, 4-hydroxyantipyrine, 3-hydroxymethylantipyrine and norantipyrine in urine. The stable isotope-labelled internal standards for the assay were obtained from a subject who ingested deuterium labelled antipyrine and produced urine containing the labelled drug and metabolites.
D000983:Antipyrine / Q000031:analogs & derivatives* / Q000652:urine*; D000077553:Edaravone; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D012015:Reference Standards; D013997:Time Factors
C007983:4-hydroxyantipyrine; C031820:3-hydroxymethylantipyrine; D000077553:Edaravone; D000983:Antipyrine