Effects of potential blood substitutes (perfluorochemicals) on rat liver and spleen.
The effect of an emulsion of perfluorochemicals (PFC) (7 parts perfluorodecalin and 3 parts perfluorotripropylamine, 4.4 g PFC/kg body weight) on organ function was determined. Whereas maximal storage of PFC was reached in the spleen as early as 12 h after PFC administration, the liver attained a maximal PFC content only after 2 days. The increase in weight also differed: a maximum occurred in the spleen on the 4th day, in the liver on the 8th day. Indocyanine green (ICG) clearance showed a small decrease, statistically significant after 12 and 24 h. Colloidal carbon clearance, used as a measure of the function of the reticuloendothelial system (RES) decreased instantly after PFC to less than half the control value; after full recovery a second decrease was seen which lasted till the 4th day after PFC. Pretreatment with C 48/80 or with increasing doses of E. coli endotoxin could largely obviate the depressive effect of PFC-loading on carbon clearance. Serum transaminases increased to about twice the control levels but were normal by the 2nd day, and thereafter. Alkaline phosphatase showed a 2.5 fold increase but returned to control level after the 2nd day. It is concluded that while a severe disturbance of liver function did not occur, the reduction in the capacity of the RES can become a serious factor in the defence against a simultaneously appearing infection if not compensated by activating the RES.
D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology*; D005466:Fluorocarbons / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects*; D008111:Liver Function Tests; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D013154:Spleen / Q000187:drug effects*; D013997:Time Factors
D001802:Blood Substitutes; D005466:Fluorocarbons