Localization of intravenously injected tumor cells in the rat mesentery after intraperitoneal administration of chemotactic stimuli.
The formation of metastases can be induced at sites injected with chemotactic factors in animals with circulating, chemotactically responsive tumor cells. This study was done to examine the possibilities that this phenomenon is due to enhanced tumor localization or stimulation of tumor growth. Walker carcinosarcoma cells labelled with 125I-iododeoxyuridine were injected into the tail veins of rats subsequently given intraperitoneal injections of chemotactic factor. 24 h later there was a 2.7- to 3.5-fold increase in the number of labelled cells in the mesenteries of animals given chemotactic factors compared to controls (p less than 0.001). The distribution of cells in other viscera was not affected by the injection. No effects were observed on the kinetics of tumor growth in vitro when cultures were supplemented with these chemotactic factors or with peritoneal lavage fluids from animals previously given intraperitoneal injections of chemotactic factor. Thus, in this model, the formation of metastases can be related to the arrest of circulating tumor cells in response to local chemotactic stimuli but the promotion of tumor growth has not been demonstrated.
D000818:Animals; D002279:Carcinoma 256, Walker / Q000473:pathology; D002630:Chemotactic Factors / Q000494:pharmacology*; D002633:Chemotaxis; D005260:Female; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D008643:Mesentery; D009362:Neoplasm Metastasis / Q000473:pathology; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology*; D010534:Peritoneal Neoplasms / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains
D002630:Chemotactic Factors