Two-dimensional analysis of human lymphocyte proteins II. Regulation of lymphocyte proteins by a molecule present in normal human urine.
Lymphocytes cultured with concentrated human urine develop many alterations (Clin. Chem. 27:1327, 1981). New proteins appear ("Urocon" proteins), while others disappear ("Urocof" proteins) from the two-dimensional gel pattern. This paper characterizes a human urinary leukocyte effector protein ("HULEP") that is responsible for the appearance of the Urocon:11-15 proteins in human lymphocytes. The effector molecule, HULEP-1, is a protein with a relative molecular mass in the range of 29000 to 43000. Urocon:11-15 production, cytoskeletal changes in the lymphocyte, and the presence of added HULEP-1 in the cultures are all directly related. This molecule appears to alter the structure of the lymphocyte by degrading actin and possible other lymphocyte cytoskeletal proteins. Peptide maps of Urocon:11-15 and beta and gamma actin provide evidence that Urocon:11-15 proteins are closely related to nonmuscle actin, and may be degradation products of this major cytoskeletal protein.
D000199:Actins / Q000032:analysis; D001345:Autoradiography; D001798:Blood Proteins / Q000032:analysis*; D002478:Cells, Cultured; D003470:Culture Media; D003599:Cytoskeleton; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000032:analysis* / Q000378:metabolism; D011507:Proteinuria
D000199:Actins; D001798:Blood Proteins; D003470:Culture Media