[Evaluation of 2 new methods for eluting antibodies from red cells].
The authors studied three techniques for eluting red cells antibodies using ether, chloroform and xylene. The xylene method produced stronger reacting eluates than ether and chloroform eluates. The advantages of xylene elution are: the method is sensitive, simple and rapid; the eluate is readily obtained from the top layer after centrifugation.
D000906:Antibodies / Q000302:isolation & purification*; D001789:Blood Group Antigens / Q000276:immunology*; D002725:Chloroform; D004912:Erythrocytes / Q000276:immunology*; D004986:Ether; D006801:Humans; D008722:Methods; D014992:Xylenes
D000906:Antibodies; D001789:Blood Group Antigens; D014992:Xylenes; D004986:Ether; D002725:Chloroform
Valutazione di due nuove metodiche per la eluizione di anticorpi gruppo-ematici.