title: Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture.
abstract: To monitor the interaction of cell surface acetylcholine (AcCho) receptors with the cytoskeleton, cultured muscle cells were labeled with radioactive or fluorescent alpha-bungarotoxin and extracted with Triton X-100, using conditions that preserve internal structure. A significant population of the AcCho receptors is retained on the skeletal framework remaining after detergent extraction. The skeleton organization responsible for restricting AcCho receptors to a patched region may also result in their retention after detergent extraction.
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002642:Chick Embryo; D003599:Cytoskeleton / Q000502:physiology*; D003902:Detergents; D008565:Membrane Proteins / Q000378:metabolism; D008854:Microscopy, Electron; D008870:Microtubules / Q000502:physiology; D009132:Muscles / Q000196:embryology*; D011485:Protein Binding; D051381:Rats; D011950:Receptors, Cholinergic / Q000378:metabolism*; D012995:Solubility
chemical_list: D003902:Detergents; D008565:Membrane Proteins; D011950:Receptors, Cholinergic