[Study of the mechanisms of protease-induced cell aggregation. I. Effect of trypsin on the aggregating capacity of chick embryo fibroblasts].
The administration of trypsin (1-100 mcg/ml) to the chick embryo fibroblast suspension, removed from the monolayer by means of EDTA, causes a pronounced aggregation of the cells. The aggregation completes within a few minutes and occurs in two steps. On the first step of the trypsin-induced aggregation, the amount of aggregating cells, grows rather slowly and monotonously, whereas on the second step, a sharp and non-linear acceleration of the aggregation process is observed. The data presented favour an assumption that the second stage of the fibroblast aggregation may be due to a structural rearrangement of cytoplasmic membranes that involves a splitting of a small, critical number of peptide bonds of cell surface proteins.
D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects*; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D002642:Chick Embryo; D005347:Fibroblasts / Q000187:drug effects*; D008565:Membrane Proteins / Q000378:metabolism; D013268:Stimulation, Chemical; D014357:Trypsin / Q000494:pharmacology*
D008565:Membrane Proteins; D014357:Trypsin
Issledovanie mekhanizmov proteazo-indutsirovannoí agregatsii kletok. I. Vliianie tripsina na sposobnost' k agregatsii fibroblastov kurinogo émbriona.