Polymer supported synthesis of oligonucleotides by a phosphotriester method.
A new polymer supported synthesis of deoxyribooligonucleotides which can be adapted to automation is described. The method is based on the elongation of an oligomer chain in the 5'- to 3' -end direction using the modified phosphotriester chemistry. The approach is exemplified by the synthesis of a nonanucleotide d(TTCGTCTTG).
D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D008722:Methods; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D011108:Polymers; D012265:Ribonucleotides
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D011108:Polymers; D012265:Ribonucleotides