[Preparation of highly purified cephamycin C].
Preparation of highly purified cephamycin C (CM-C) is described in this report. The purification of CM-C was carried out by Sephadex LH-20 chromatography of the N-BOC-CM-C developed with MeOH followed by removal of the protecting group. Partially purified CM-C was further refined by Sephadex LH-20 chromatography developed with MeOH and Diaion HP-20 SS chromatography developed with H2O. CM-C thus obtained showed the UV absorption at 265 nm [E1% 1cm 184.4 (H2O)] which is the strongest absorption ever appeared in the literatures.
D002511:Cephalosporins / Q000302:isolation & purification*; D002513:Cephamycins / Q000302:isolation & purification*; D008722:Methods
D002511:Cephalosporins; D002513:Cephamycins; C024925:cephamycin C