[Detection of complement-binding immune complexes by means of 51Cr-labeled indicator cells--a new radioimmunoassay (author's transl)].
A new radioimmunoassay for the detection of complement-binding immune complexes is described. The method is based on the ability of the complexes to bind added guinea pig complement (Clq-Component) so that is not available for lysis of 51Cr-labeled chicken red blood cells in a haemolytic system. Sera from 45 patients with malignant tumours, 20 patients with nonmalignant diseases were studied for the presence of circulating immune complexes. The complement-binding capacity of the sera of patients with significantly greater than that of control sera.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis*; D001172:Arthritis, Rheumatoid / Q000175:diagnosis; D002645:Chickens / Q000276:immunology; D002860:Chromium Radioisotopes; D003165:Complement System Proteins; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis; D011863:Radioimmunoassay / Q000379:methods*
D000936:Antigen-Antibody Complex; D002860:Chromium Radioisotopes; D003165:Complement System Proteins
Nachweis Komplement-bindender Immunkomplexe mittels 51Cr-markierter Indikatorzellen--eine neue radioimmunologische Bestimmungsmethode.