Differential pulse polarography of some degradation products of tetracycline.
In an attempt to develop a more rapid, convenient, and precise method for the direct detection and analysis of the degradation products of tetracycline, a study of those products utilizing differential pulse polarography was initiated. The investigation was concentrated on the subject of the kinetics of the epimerization of anhydrotetracycline to 4-epianhydrotetracycline in acetate buffer. The reaction was followed at 25 and 50 degrees. Duplicate experiments were run at each temperature. The apparent rate constants obtained were 4.17 +/- 0.13 X 10(-1)/hr (25 degrees) and 6.97 +/- 1.00 X 10(-2)/hr (50 degrees).
D001711:Biotransformation; D007700:Kinetics; D011048:Polarography / Q000379:methods; D013237:Stereoisomerism; D013754:Tetracyclines / Q000032:analysis*
D013754:Tetracyclines