Radioimmunoassay for human testosterone-estradiol-binding globulin.
We isolated testosterone-estradiol-binding globulin (TeBG) from human pregnancy plasma and used the isolated protein to obtain a rabbit antiserum to it. Using immunochemical techniques, the antiserum was demonstrated to be both monospecific and to have the ability to remove TeBG-binding activity from human serum. This antiserum was used to develop a RIA for human TeBG. The sensitivity is such that TeBG can be determined in as little as 0.005 microliter serum or plasma, although we routinely use 0.5-1.0 microliter. The correlation between the RIA and an established binding assay, using 63 different specimens, was 0.995.
D000818:Animals; D005260:Female; D006801:Humans; D008297:Male; D011247:Pregnancy; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D012016:Reference Values; D012738:Sex Hormone-Binding Globulin / Q000032:analysis*
D012738:Sex Hormone-Binding Globulin