[Comparative effectiveness of using different tests to determine the mutagenicity of certain factors in mammals. I. Frequency of dominant lethal mutations and chromosome aberrations in mouse bone marrow cells induced by N-nitrosomorpholine, N-nitroso-N-methylurea and cyclophosphamide].
Two tests (the frequency of dominant lethal mutations at different stages of spermatogenesis and the yield of chromosome aberrations in bone marrow cells) were used to investigate mutagenisity of N-nitrosomorpholine (NM), N-nitroso-N-methylurea (NMU) and cyclophosphamide (CTX). The drugs were injected once intraperitoneally to male mice (F1 of CBA X C57BL) at doses of NM--50 mg/kg; NMU--50 mg/kg; CTX--50, 100, 200 and 300 mg/kg. It was established that NM was not mutagenic in the mouse as measured by these two tests. NMU was a weak mutagen at the stated exposure, since there was only slight increase in the frequency of dominant lethal mutations in late spermatids and spermatogonia, and also a low yield of chromosome aberrations in bone marrow cells was induced. CTX had a strong mutagenic effect on postmeiotic stages of spermatogenesis and in bone marrow cells.
D000818:Animals; D001853:Bone Marrow / Q000648:ultrastructure; D002869:Chromosome Aberrations; D003520:Cyclophosphamide / Q000633:toxicity*; D005260:Female; D005313:Fetal Death; D005799:Genes, Dominant; D005804:Genes, Lethal; D006824:Hybridization, Genetic; D008297:Male; D008770:Methylnitrosourea / Q000633:toxicity*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009025:Morpholines / Q000633:toxicity*; D009152:Mutagenicity Tests; D009602:Nitrosamines / Q000633:toxicity*; D009607:Nitrosourea Compounds / Q000633:toxicity*; D011247:Pregnancy; D013094:Spermatozoa / Q000648:ultrastructure
D009025:Morpholines; D009602:Nitrosamines; D009607:Nitrosourea Compounds; C002741:N-nitrosomorpholine; D008770:Methylnitrosourea; D003520:Cyclophosphamide
Sravnitel'naia éffektivnost' ispol'zovaniia raznykh testov dlia opredeleniia mutagennosti nekotorykh faktorov u mlekopitaiushchikh. Soobshchenie I. Chastota dominantnykh letal'nykh mutatsiĭ i khromosomnykh aberratsiĭ v kletkakh kostnogo mozga myshi, indutsirovannykh N-nitrozomorfolinom, N-nitrozo-N-metilmichevinoĭ i tsiklofosfamidom.