Influence of thiopental on intracellular distribution of hexokinase activity in various tumor cells.
In earlier work it has been shown that mitochondrially bound brain hexokinase is solubilized by anesthetics. This effect was reevaluated using cultured cells. For the present experiments Ehrlich ascites, Harding-Passey melanoma, C-1300-neuroblastoma and C-6-glioma cells were used. The great portion of hexokinase activity bound to the mitochondria of these cells was similar to that in rat brain. After incubation with thiopental the soluble hexokinase activity was increased in all cells studied. Using neuroblastoma and glioma cells the thiopental effect was demonstrated to be dose-dependent. Thus, cultured tumor cells seem to be useful for studying the relationship of the intracellular distribution of hexokinase activity, energy metabolism and the effect of anesthetics.
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006593:Hexokinase / Q000378:metabolism*; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis; D009374:Neoplasms, Experimental / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology*; D013874:Thiopental / Q000494:pharmacology*
D009363:Neoplasm Proteins; D006593:Hexokinase; D013874:Thiopental