Effect of blood derived monocytes on the promotion of in vitro erythropoietic colony growth in human bone marrow cultures.
The effect of monocytes on the promotion of erythropoietic burst formation in human bone marrow methylcellulose cultures was studied in a burst-promoting activity (BPA)-poor system. Irradiated Ficoll-Isopaque separated blood mononuclear cells stimulated BFU-E growth proportionally to the number of irradiated cells added. Monocyte-depleted mononuclear cells stimulated as well as total mononuclear cells. Monocyte-concentrates stimulated when added at low concentrations. Monocyte-depleted mononuclear cells, although stimulatory at higher concentrations, did not stimulate at the concentrations used for monocyte-concentrates.
D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004920:Erythropoiesis; D006133:Growth Substances / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D009000:Monocytes / Q000502:physiology*
D006133:Growth Substances