Comparative mutagenicity of 4 DNA-intercalating agents in L5178Y mouse lymphoma cells.
The mutagenicity of 4 known intercalating agents acridine orange (AO), quinacrine mustard (QM), proflavin (PF) and ethidium bromide (EB) has been investigated in L5178Y mouse lymphoma cells. Methyl methanesulfonate (MMS) was used as a positive control in these studies. AO, QM and PF induced mutation in the excess thymidine- and thioguanine-selective systems. These 3 compounds were negative in the ouabain- and cytosine-arabinoside-selective systems while EB was positive only in the cytosine arabinoside system. It would appear that the EB-induced mutagenesis is different from that of AO, QM and PF though all are intercalating agents. Since the molecular origin of cytosine arabinoside mutants is unknown, further interpretation of the EB results is not possible.
D000165:Acridine Orange / Q000494:pharmacology*; D000166:Acridines / Q000494:pharmacology*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004996:Ethidium / Q000494:pharmacology*; D007364:Intercalating Agents / Q000494:pharmacology*; D007940:Leukemia L5178 / Q000235:genetics* / Q000503:physiopathology; D007942:Leukemia, Experimental / Q000235:genetics*; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D051379:Mice; D009153:Mutagens; D009154:Mutation; D011370:Proflavine / Q000494:pharmacology*; D011796:Quinacrine / Q000031:analogs & derivatives*; D011797:Quinacrine Mustard / Q000494:pharmacology*
D000166:Acridines; D007364:Intercalating Agents; D009153:Mutagens; D011797:Quinacrine Mustard; D008741:Methyl Methanesulfonate; D011370:Proflavine; D004996:Ethidium; D000165:Acridine Orange; D011796:Quinacrine