Specific inducibility of globin mRNA in Friend leukemia cells in low serum concentration.
Friend leukemia cells were adapted to grow in a medium containing 0.5% serum by gradually decreasing concentration of the serum from 15%. Upon treatment of the cells with dimethyl sulfoxide, appearance of benzidine-positive cells was suppressed in the cells cultivated in a 0.5% serum medium, whereas globin mRNA sequences, as determined by hybridization of cytoplasmic RNA with globin cDNA, increased to the same order as in the cells grown in a medium containing 15% serum. A small but significant amount of globin was synthesized in cells treated with dimethyl sulfoxide in 0.5% serum medium, as determined by dodecyl sulfate-polyacrylamide gel electrophoresis of 3H-labeled cellular proteins.
D000818:Animals; D001560:Benzidines; D001769:Blood; D002460:Cell Line; D003470:Culture Media; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005622:Friend murine leukemia virus; D005914:Globins / Q000096:biosynthesis*; D006454:Hemoglobins / Q000096:biosynthesis; D007942:Leukemia, Experimental / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*
D001560:Benzidines; D003470:Culture Media; D006454:Hemoglobins; D012333:RNA, Messenger; D005914:Globins; D004121:Dimethyl Sulfoxide