Enumeration and characterization of bovine blood, spleen and lymph node cells containing immunoglobulins.
In this study, the average percentage of bovine spleen and lymph node cells with surface immunoglobulin (S-Ig) was found to be 19.62 and 23.18%, respectively. The average percentage of these cells with cytoplasmic immunoglobulin (C-Ig) was 14.46 and 17.21%. Only the percentage of cells with S-Ig showed a strong correlation between the spleen and the lymph node. Also, several methods of removing S-Ig from bovine peripheral blood mononuclear cells were investigated. It was found that the loss of passively bound S-Ig by warm washing was minimal, and that the anti-Ig-treated mononuclear blood cells would lose their caps after 24 h of incubation at 37 degrees C, but not after 45 min of incubation, although 95% of the cells with S-Ig were capped within 15 min.
D000818:Animals; D000921:Antibody-Producing Cells / Q000166:cytology / Q000276:immunology*; D002417:Cattle; D002452:Cell Count; D003593:Cytoplasm / Q000276:immunology; D005260:Female; D007136:Immunoglobulins; D007152:Immunologic Capping; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell; D013154:Spleen / Q000166:cytology / Q000276:immunology*
D007136:Immunoglobulins; D011947:Receptors, Antigen, B-Cell