An esterase isozyme in non-hemoglobin proteins of cell lysate reacting with mushroom lectin.
Of 20 kinds of plant lectins examined, agglutinin activity of only mushroom lectin was inhibited with non-hemoglobin proteins of the red cell lysate. Chromatography of non-hemoglobin proteins on DEAE-cellulose and on hydroxylapatite resulted in isolating a protein which gave a single precipitation line with esterase activity in immunoelectrophoresis. SDS-polyacrylamide gel disc electrophoresis demonstrated that the protein consisted of two subunits molecular weight of which was approximately 100,000 each.
D000371:Agglutination; D001487:Basidiomycota; D004912:Erythrocytes / Q000201:enzymology*; D004950:Esterases / Q000302:isolation & purification*; D007122:Immunoelectrophoresis; D007527:Isoenzymes / Q000302:isolation & purification*; D037102:Lectins / Q000276:immunology*
D007527:Isoenzymes; D037102:Lectins; D004950:Esterases