[Effects of pH and ionic strength on interaction of different histone fractions and protamine with immobilized histone H4].
The interaction of histone H4 immobilized on Sepharose 4B with protamine and individual and total histone fractions from calf thymus has been studied. In accordance with the strength of their interaction with histone H4, the individual histone fractions can be arranged in the following order: H4 greater than H2a greater than H2b greater than H1 and protamine. The structure of the complexes, which are formed in a solution of a total histone fraction and specifically interact with the immobilized histone H4, depends on the ionic composition and pH of the medium.
D000818:Animals; D002417:Cattle; D006657:Histones / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D011479:Protamines; D011485:Protein Binding; D012685:Sepharose; D013950:Thymus Gland
D006657:Histones; D011479:Protamines; D012685:Sepharose
Vliianie pH i ionnoĭ sily sredy na vzaimodeĭstvie razlichnykh fraktsiĭ gistonov i protamina s immobilizovannym gistonom H4.