Effects of sodium picosulfate, bisacodyl and sennoside in cultured human, rat and rabbit liver cells.
Cultured liver cells of man, rat and rabbit were exposed to sodium picosulfate using medium concentrations of 400, 800,1000,1600, or 2000 micrograms/ml, and the results were compared with findings after bisacodyl at 25, 50 or 100 micrograms/ml and sennoside at 100 or 200 micrograms/ml. Much higher concentrations of sodium picosulfate than of both comparative substances were tolerated. At the doses used, hepatocytes of rabbit were slightly more sensitive against sodium picosulfate, while effects of bisacodyl and sennoside on hepatocytes were comparable between the three species used. No substance-related deviations in leakage of LDH from liver cells into culture medium were observed. The protein content was lowered in liver cells of rabbit exposed to 1600 micrograms/ml sodium picosulfate and the human cells exposed to 100 micrograms/ml bisacodyl. An increase in spindle-shaped growth occurred only in human cells exposed to bisacodyl.
D000818:Animals; D000880:Anthraquinones / Q000494:pharmacology; D001726:Bisacodyl / Q000494:pharmacology; D002400:Cathartics / Q000494:pharmacology*; D002478:Cells, Cultured; D002951:Citrates; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D009942:Organometallic Compounds; D010847:Picolines / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D012676:Senna Extract; D000081226:Sennosides; D013045:Species Specificity
D000880:Anthraquinones; D002400:Cathartics; D002951:Citrates; D009942:Organometallic Compounds; D010847:Picolines; D011506:Proteins; D000081226:Sennosides; D001726:Bisacodyl; D012676:Senna Extract; D007770:L-Lactate Dehydrogenase; C005701:picosulfate sodium