Biosynthesis and processing of a human precursor complement protein, pro-C3, in a hepatoma-derived cell line.
A 180,000-dalton single-chain molecule (human pro-C3) is the precursor of the third component of human complement (C3), a disulfide-linked two-chain protein. The pro-C3 is converted by limited proteolysis to C3. The relationship between pro-C3 and C3 was established with the use of Hep G2, a cell line derived from a human hepatocellular carcinoma, which synthesizes at least 17 plasma proteins.
D000818:Animals; D002460:Cell Line; D003176:Complement C3 / Q000096:biosynthesis*; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D046911:Macromolecular Substances; D008970:Molecular Weight; D011498:Protein Precursors / Q000378:metabolism
D003176:Complement C3; D046911:Macromolecular Substances; D011498:Protein Precursors