Allergenicity of major component proteins of soybean.
Allergenic activities of major component proteins of soybean 11S-, 7S- and 2S-globulins were examined by the radioallergosorbent test (RAST) and by RAST inhibition experiments with the sera from soybean-sensitive patients. The reactivity with specific IgE antibody was found in all fractions. There was considerable cross-reactivity among three fractions, and 2S-globulin had the highest allergen potency to inhibit the RAST against 11S, 7S- and 2S-globulin. When the fractions were heat-treated at 80, 100 and 120 degrees C for 30 min, the RAST reactivity of 2S-globulin heated at 80 degrees C for 30 min was slightly enhanced, while that of others was reduced to 39--75% of that of the native globulin. IgE antibody could be produced in BALB/c mice only with one of the three fractions, i. e. 2S-globulin, and the produced IgE antibody lacked reactivity with 11S- and 7S-globulin.
D000485:Allergens; D000818:Animals; D001667:Binding, Competitive; D002648:Child; D002675:Child, Preschool; D003429:Cross Reactions; D005916:Globulins / Q000302:isolation & purification; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008925:Mites; D010047:Ovalbumin / Q000276:immunology; D010323:Passive Cutaneous Anaphylaxis; D010940:Plant Proteins / Q000276:immunology*; D011852:Radioallergosorbent Test; D013025:Glycine max
D000485:Allergens; D005916:Globulins; D010940:Plant Proteins; D007073:Immunoglobulin E; D010047:Ovalbumin