Superoxide dismutase in extracellular fluids.
Serum from healthy volunteers contained very little CuZn superoxide dismutase. Larger amounts were found in serum from patients with impaired renal function, and there was a good correlation between serum creatinine and serum CuZn superoxide dismutase content. Almost all superoxide dismutase activity of human serum was cyanide sensitive, and was found to be given by a factor(s) with a molecular mass of approximately 130000. The factor was found in all human extracellular fluids investigated: plasma, serum, lymph, ascites and cerebrospinal fluid. The factor was not recognized by radioimmunoassay for human CuZn superoxide dismutase, and it was not inhibited by rabbit antibodies against human CuZn superoxide dismutase. A similar high-molecular mass factor was found in plasma from all mammals investigated: horse, cow, pig, dog, cat, rabbit, rat and mouse. The activities differed much among species and were mostly higher than those found in human serum/plasma.
D000818:Animals; D001202:Ascitic Fluid / Q000201:enzymology; D001826:Body Fluids / Q000201:enzymology*; D002415:Cats; D002417:Cattle; D002850:Chromatography, Gel; D003300:Copper; D003486:Cyanides / Q000494:pharmacology; D004285:Dogs; D006736:Horses; D006801:Humans; D007674:Kidney Diseases / Q000201:enzymology; D008196:Lymph / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013552:Swine; D015032:Zinc
D003486:Cyanides; D003300:Copper; D013482:Superoxide Dismutase; D015032:Zinc