Correlation between a specific molecular weight form of plasminogen activator and metabolic activity of 3T3 cells.
In quiescent cultures of 3T3 cells, plasminogen activator (PA) is found predominantly as a 75,000 dalton species. When quiescent cells are exposed to mitogenic agents such as phorbol myristate acetate, Ca++, or 25% serum, the absolute levels of PA in cell lysates may either increase or decrease. However, a consistent observation is that in the stimulated cultures PA is found predominantly as a 49,000 dalton species. This also is the predominant form of PA in growing and transformed cells. Concomitant with the mitogen-induced stimulation of the 49,000 dalton PA in quiescent cultures is a change in morphology to one that is characteristic of growing and transformed cells. The data suggest that PA is not operative in causing the morphological change that occurs with activation; however, the 49,000 dalton PA in particular is closely related to the pleiotypic response accompanying growth stimulation and transformation.
D000818:Animals; D001769:Blood; D002118:Calcium / Q000494:pharmacology; D002455:Cell Division; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured / Q000166:cytology / Q000378:metabolism*; D007399:Interphase; D051379:Mice; D008934:Mitogens / Q000494:pharmacology; D008970:Molecular Weight; D010960:Plasminogen Activators / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology
D008934:Mitogens; D011480:Protease Inhibitors; D010960:Plasminogen Activators; D002118:Calcium