Incorporation of 7-ketocholesterol by plasma membranes from porcine aortic endothelial cells.
We present evidence that 7-ketocholesterol (7KC) is incorporated into the plasma membranes of endothelial cells in excised porcine aortas in molar amounts comparable to the native cholesterol present in the membrane. Our experimental findings indicate that simple adsorption of 7KC does not play a role in the results obtained. Instead 7KC appears to be inserted into the lipid bilayer of the membranes. This is largely independent of membrane cholesterol content and is not due to substitution of the native membrane cholesterol. A significant increase in the total sterol-to-phospholipid ratio is obtained with 7KC-exposure without significant changes in the cholesterol-to-phospholipid ratio.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000378:metabolism; D003470:Culture Media; D046508:Culture Techniques; D004727:Endothelium / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D007653:Ketocholesterols / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D013347:Subcellular Fractions; D013552:Swine
D003470:Culture Media; D007653:Ketocholesterols; D008563:Membrane Lipids; D010743:Phospholipids; D002784:Cholesterol; D000469:Alkaline Phosphatase; C003001:7-ketocholesterol