title: IgE antibody-mediated cytotoxicity of rat macrophages against microfilaria of Dipetalonema citeae in vitro.
abstract: The Fischer rat develops an acquired resistance against circulating microfilariae. Macrophages from the peritoneal washings of normal rats preincubated at 37 degrees C with the sera obtained from rats immune to circulating microfilariae adhered to and kill the microfilaria of Dipetalonema viteae in vitro within 16 to 24 hr. No significant adherence and cytotoxicity was mediated by sera collected from animals with microfilaraemia or from normal rats. Adherence of macrophages to microfilaria was associated with damage to the surface of the larva as revealed by ultrastructural studies. Neither adherence nor cytotoxicity was induced by preincubation of microfilariae, instead of macrophages with immune serum. The serum factor which mediated adherence and cytotoxicity was heat-labile, but was not a complement component. Immune absorption experiments showed that the relevant serum factor resided in the IgE class of antibody. The immune adherence to D. viteae by macrophages is stage-specific because adherence to infective larvae was not observed whether rate macrophages were preincubated in sera obtained from rats immune to microfilariae or in sera collected from animals after exposure to infective larvae.
mesh_terms: D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D001202:Ascitic Fluid / Q000276:immunology; D002448:Cell Adhesion; D004153:Dipetalonema / Q000276:immunology*; D004154:Dipetalonema Infections / Q000276:immunology; D007106:Immune Sera; D007110:Immunity, Active; D007073:Immunoglobulin E / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D008842:Microfilariae / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007106:Immune Sera; D007073:Immunoglobulin E