Studies on Alternaria allergens. III. Isolation of a mafor allergenic fraction (ALT-I).
The allergens present in crude Alternaria alternata extract were fractionated by sequential ammonium sulfate precipitation, DEAE cellulose ion-exchange chromatography, preparative flat-bed electrofocusing, and Sephadex G-100 gel filtration. The carbohydrate-rich fraction isolated (Alt-I) was heterogeneous by polyacrylamide gel disc electrophoresis and by thin-layer electrofocusing. By preparative polyacrylamide gel electrophoresis the Alt-I fraction could be further separated into at least five subfractions, all of which contained similar allergenic activity. Significant elevations in IgE antibody to Alt-I were found in 21
D000485:Allergens / Q000302:isolation & purification*; D000528:Alternaria / Q000276:immunology*; D001249:Asthma / Q000175:diagnosis; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D003904:Mitosporic Fungi / Q000276:immunology*; D008970:Molecular Weight; D011852:Radioallergosorbent Test; D012882:Skin Tests
D000485:Allergens