In vivo distribution of proteins within a single cell.
Using the oocyte of Xenopus laevis, we present an experimental system, involving two-dimensional gel electrophoresis, for measuring unambiguously the nucleocytoplasmic distribution of proteins within a living cell.
D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis*; D003593:Cytoplasm / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007525:Isoelectric Focusing; D008832:Microchemistry; D008970:Molecular Weight; D009698:Nucleoproteins / Q000032:analysis; D009865:Oocytes / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D014981:Xenopus
D009698:Nucleoproteins; D011506:Proteins