[Preparation of a polyvalent antiserum specific for human muscle proteins. II. Animal immunization and purification of the antiserum].
A new preparation technique to raise rabbit antisera against human water-soluble muscle proteins is presented. Immunization, blood collection and further purification of antibody fraction in serum are described in detail. The present method is fast and easy to perform; chromatography and dialysis are not required and antisera so produced lend themselves to immunoelectrophoretic evaluation of human water-soluble muscle proteins.
D000818:Animals; D001800:Blood Specimen Collection; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification*; D007122:Immunoelectrophoresis; D009124:Muscle Proteins / Q000032:analysis / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D014462:Ultrafiltration
D007106:Immune Sera; D009124:Muscle Proteins
Preparazione di un antisiero polivalente specifico per le proteine muscolari umane. II. Immunizzazione degli animali e purificazione dell'antisiero ottenuto.