Some improvements in the separation technique for the chemical syntheses of oligodeoxyribonucleotides.
Some improvements have been achieved for the separation technique in the syntheses of oligodeoxyribonucleotides by a solid phase phosphotriester method; (1) reuse of the protected nucleotides recovered from the reaction mixture by a preparative GPC. (2) a new weak anion exchange column, HPLC-DEAE, was used to estimate the size of the deblocked oligomers.
D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000302:isolation & purification*; D009841:Oligonucleotides / Q000302:isolation & purification*
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D004247:DNA