Formation of hybridoma clones in soft agarose: effect of pH and of medium.
Optimal conditions for the formation of hybridoma clones in soft agarose are described. The hybridization frequency is shown to be highly dependent on the pH of the polyethylene glycol (PEG) solution used for fusion and on the cloning medium. Maximal numbers of clones are obtained when the PEG solution used for fusion is at pH 8.0-8.2.
D000818:Animals; D000921:Antibody-Producing Cells / Q000166:cytology; D002459:Cell Fusion / Q000187:drug effects*; D002999:Clone Cells / Q000166:cytology*; D003470:Culture Media; D006822:Hybrid Cells / Q000166:cytology*; D006863:Hydrogen-Ion Concentration; D051379:Mice; D009194:Myeloma Proteins; D009374:Neoplasms, Experimental / Q000473:pathology; D010954:Plasmacytoma / Q000473:pathology; D011092:Polyethylene Glycols / Q000494:pharmacology; D012685:Sepharose
D003470:Culture Media; D009194:Myeloma Proteins; D011092:Polyethylene Glycols; D012685:Sepharose