Changes in polysomes and mRNA in ageing soybean (Glycing max L.) cotyledons.
Polysomes and their associated ribonucleic acids were extracted from ageing soybean cotyledons. Density gradient analysis showed that the ribosomes of older cotyledons were present largely as monosomes and light polysomes while younger cotyledons possessed a high proportion of heavy polysomes. Increased polysomal and mRNA activities were observed in 3- to 5-day-old and again in 10-day-old cotyledons. The presence of polyadenylated mRNA was determined by oligo (dT) cellulose chromatography and confirmed by hybridization with 3H-polyuridylic acid.
D002846:Chromatography, Affinity; D011132:Polyribosomes / Q000502:physiology*; D012333:RNA, Messenger / Q000502:physiology*; D013025:Glycine max / Q000648:ultrastructure*; D013997:Time Factors
D012333:RNA, Messenger