Overestimate of 125I-protein uptake from the adult mouse gut.
Gastric instillation of 125I-labelled protein in mice leads to rapid appearance of blood radioactivity which is precipitable with 10% trichloroacetic acid (TCA). The same occurs when Na125I is given by mouth. Parenteral administration of non-radioactive NaI before feeding Na125I or 125I-labelled protein inhibits the appearance of TCA-precipitable radioactivity in the blood. The results presented in this study show that measurement of TCA-precipitable radioactivity in the blood after feeding 125I-labeled protein is not a reliable method for studying the passage of macromolecules across the gastrointestinal barriers unless an excess of iodide is given.
D000818:Animals; D011232:Chemical Precipitation; D004136:Dinitrobenzenes / Q000378:metabolism; D005260:Female; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012711:Serum Albumin, Radio-Iodinated / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D012974:Sodium Iodide / Q000378:metabolism / Q000494:pharmacology; D014238:Trichloroacetic Acid
D004136:Dinitrobenzenes; D007457:Iodine Radioisotopes; D012711:Serum Albumin, Radio-Iodinated; D012965:Sodium Chloride; D014238:Trichloroacetic Acid; D012974:Sodium Iodide