Complementary recessive 25-hydroxycholesterol-resistant somatic cell mutants--assay of 25-hydroxycholesterol binding activity.
Complementation analysis of recessive 25-hydroxycholesterol-resistant mutants of the CHO-Kl cell shows the existence of at least two complementation groups, one of which is missing a binding activity for 25-hydroxycholesterol. Both complementation groups are shown to be refractory to inhibition of cellular HMG-CoA reductase activity and in the inhibition of biosynthesis of this enzyme by 25-hydroxycholesterol.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D005260:Female; D005808:Genes, Recessive; D005816:Genetic Complementation Test; D006822:Hybrid Cells; D006888:Hydroxycholesterols / Q000378:metabolism* / Q000494:pharmacology; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D009154:Mutation
D006888:Hydroxycholesterols; C007997:25-hydroxycholesterol; D006903:Hydroxymethylglutaryl CoA Reductases