The identification of Y chromosome translocations following Distamycin A treatment.
Pretreatment of PHA-stimulated lymphocyte cultures from normal males with 100 micrograms/ml Distamycin A for the final 24 h of incubation led to a striking decondensation of the heterochromatic portion of the long arm of the Y chromosome. The despiralized region of Yq maintained its fluorescent properties in this altered morphologic state. Two cases of Y chromosome translocation (Y/22 and Y/X) were identified with this technique. A review of the literature revealed 62 similar cases in which the phenotypic-karyotypic correlations were instrumental in the prenatal counseling of the family with the Y/22 translocation.
D002871:Chromosome Banding; D002904:Chromosomes, Human, 21-22 and Y; D004214:Distamycins; D005260:Female; D006801:Humans; D008297:Male; D011247:Pregnancy; D011296:Prenatal Diagnosis; D011758:Pyrroles; D012730:Sex Chromosomes; D014178:Translocation, Genetic; D014998:Y Chromosome
D004214:Distamycins; D011758:Pyrroles