Thermal denaturation of distamycin A--DNA complexes as followed by hyperchromic spectra.
Interaction of distamycin A with calf spleen DNA is investigated by the method of hyperchromic spectra. Hyperchromic spectra of complexes are partitioned into the components corresponding to the denaturation A . T and G . C base pairs and dissociation of the ligand, fractions of respective components are found as a function of temperature. A scheme of melting of successive regions of DNA with different G+C content together with the scheme of distamycin A redistribution in the course of thermal denaturation is presented.
D000818:Animals; D002417:Cattle; D004247:DNA; D004214:Distamycins; D009691:Nucleic Acid Denaturation; D011758:Pyrroles; D013056:Spectrophotometry, Ultraviolet; D013154:Spleen; D013696:Temperature
D004214:Distamycins; D011758:Pyrroles; D004247:DNA