title: Antibodies to N-(guanosin-8-yl)-2-aminofluorene.
abstract: Antibodies to Guo-AF were elicited in rabbits by immunization with bovine serum albumin -Guo-AF conjugate. The antibodies were purified by affinity chromatography. The affinity of the antibodies towards several ligands was studied by radioimmunoassays. The antibodies recognize Gua-AAF and Guo-AF (K(Guo-AF)/K(Guo-AAF) congruent to 25). The circular dichroism of Guo-AF as a function of pH is reported.
mesh_terms: D000818:Animals; D000906:Antibodies; D002417:Cattle; D002942:Circular Dichroism; D003429:Cross Reactions; D005449:Fluorenes / Q000276:immunology*; D008024:Ligands; D008968:Molecular Conformation; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine; D013329:Structure-Activity Relationship
chemical_list: D000906:Antibodies; D005449:Fluorenes; D008024:Ligands; D012710:Serum Albumin, Bovine; C026478:N-(guanosin-8-yl)-2-aminofluorene