Replicon fusion in mouse cells is very sensitive to DNA damage induced by N-methyl-N'-nitro-N-nitrosoguanidine.
Incubation of mouse cells with N-methyl-N'-nitro-N-nitrosoguanidine causes a strong inhibition of DNA replication the extent of which varies with the cell line used. Analysis of the products synthesized in drug-treated cells indicates a particularly severe effect on the joining of replicons while other steps in DNA synthesis like initiation and chain elongation are much less affected. The data indicate that replicon fusion may be extremely sensitive to changes in the topology of DNA induced by the introduction of rare single-strand breaks during repair of N-methylated purines produced by incubation of cells with small amounts of the methylating agent.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004261:DNA Replication; D005347:Fibroblasts / Q000378:metabolism; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D051379:Mice; D012093:Replicon / Q000187:drug effects*
D008769:Methylnitronitrosoguanidine