Methionine-defective mutants in Candida utilis.
Ethionine-resistant mutants of Candida utilis CCY-158 overproducing methionine have been isolated. In these mutants the intracellular methionine concentration decreased significantly during the stationary phase. The wild-type strain CCY-158 and the ethionine-resistant mutants isolated were able to use methionine as the nitrogen source but not as the carbon source. From these ethionine-resistant mutants we isolated mutants unable to use methionine as nitrogen source (Mec- mutants), the principal alteration being at the level of methionine uptake. Some of the Mec mutants lost also the ability to use other amino acids as nitrogen source.
D002175:Candida / Q000254:growth & development / Q000378:metabolism*; D005001:Ethionine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism*; D009154:Mutation
D008715:Methionine; D005001:Ethionine