title: Direct detection of two cross-reactive antigens between porcine and rabbit zonae pellucidae by radioimmunoassay and immunoelectrophoresis.
abstract: Zonae pellucidae were isolated from oocytes obtained from rabbit and porcine ovaries. Antisera were prepared in rabbits against heat-solubilized porcine zonae and in guinea pigs against intact and heat-solubilized rabbit zonae and against purified rabbit zona proteins isolated from two-dimensional electrophoresis gels. These antisera were used in radioimmunoassay studies to show cross-reactivity between porcine and rabbit zona antigens. Immunoelectrophoresis studies also demonstrated the immunochemical cross-reactivity between two distinct antigens of rabbit and porcine zonae. These two rabbit zona antigens were demonstrated to be immunochemically identical to porcine zona antigens by crossed-line immunoelectrophoresis.
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis*; D003429:Cross Reactions; D005260:Female; D007122:Immunoelectrophoresis; D010063:Ovum / Q000276:immunology*; D011817:Rabbits / Q000276:immunology*; D011863:Radioimmunoassay; D013552:Swine / Q000276:immunology*; D015044:Zona Pellucida / Q000276:immunology*
chemical_list: D000941:Antigens