Histofluorescence analysis of several systems of catecholaminergic nerve fibres within the rat neostriatum revealed by either restricted lesions of the substantia nigra or gamma-hydroxybutyrate.
The glyoxylic acid fluorescence technique was applied to the study of catecholamine fibres in the caudate-putamen (neostriatum) of control and experimental adult rats. Cryostat or vibratome sectioning procedures were used; in the last case, either incubations of brain slices in gamma-methyl-noradrenaline or pre-treatment of animals with gamma-hydroxybutyrate were performed, in order to increase the intensity of fluorescence. In control animals and in the contralateral side of rats lesioned in the substantia nigra, the fluorescence of the dense plexus of dopamine nerve fibres appeared under the form of densely packed varicosities. However, some regions differed from the ordinary fluorescence of the neostriatum by their stronger intensity and more visible varicosities. They were located principally in the ventro-medial regions bordering either the nucleus accumbens, the dorsal nucleus interstitialis striae terminalis and the ventricle in the anterior part, or the amygdala, the globus pallidus and the ventricle in the posterior part. Moreover, islands of stronger fluorescence also were observed in the head of the caudate-putamen along the dorsal and lateral corpus callosum, as well as scattered in the central region. These particular neostriatal structures might correspond to a separate system of ascending dopamine nerve fibres. Therefore, restricted electrolytic lesions of the substantia nigra pars compacta were undertaken in order to study the morphology of the remaining catecholaminergic fibres in the ipsilateral neostriatum. In largely denervated areas, different types of fluorescent axons were evident. Presumed noradrenergic nerve fibres characterized by a very coarse appearance and closed varicosities were rare. They contrasted markedly with numerous delicate fibres which might belong to several dopaminergic systems. Both first types exhibited long, clearly visible intervaricose segments and ovoid triangular varicosities. The second type, which was thicker and more strongly fluorescent, probably formed the islands observed in unlesioned striata. The third type had very closed, small spherical varicosities and poorly fluorescent intervaricose segments. Dopamine nerve fibres of the same morphology were described previously in the neocortex [43,44] and the possibility of a common origin for nerve fibres of the same type is discussed. When rats were treated with the anaesthetic gamma-hydroxybutyrate, all the dopamine nerve fibres appeared to develop a strong fluorescence within the neostriatum, but more fluorescent islands were always visible. Additionally, some swollen fluorescent fibres were seen; these could be abnormal dopamine fibres whose metabolism had been pathologically altered by the drug.
D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D002421:Caudate Nucleus / Q000033:anatomy & histology; D003342:Corpus Striatum / Q000033:anatomy & histology* / Q000187:drug effects; D004298:Dopamine / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009412:Nerve Fibers / Q000648:ultrastructure*; D009434:Neural Pathways / Q000033:anatomy & histology; D009638:Norepinephrine / Q000378:metabolism; D011699:Putamen / Q000033:anatomy & histology; D051381:Rats; D012978:Sodium Oxybate / Q000494:pharmacology; D013378:Substantia Nigra / Q000033:anatomy & histology* / Q000502:physiology
D002395:Catecholamines; D012978:Sodium Oxybate; D004298:Dopamine; D009638:Norepinephrine