Rapid radiometric method for detection of Salmonella in foods.
A radiometric method for the detection of Salmonella in foods has been developed which is based on Salmonella poly H agglutinating serum preventing Salmonella from producing 14CO2 from [14C]dulcitol. The method will detect the presence or absence of Salmonella in a product within 30 h compared to 4 to 5 days by routine culture methods. The method has been evaluated against a routine culture method using 58 samples of food. The overall agreement was 91%. Five samples negative for Salmonella by the routine method were positive by the radiometric method. These may have been false positives. However, the routine method may have failed to detect Salmonella due to the presence of large numbers of lactose-fermenting bacteria which hindered isolation of Salmonella colonies on the selective agar plates.
D000818:Animals; D002250:Carbon Radioisotopes; D002645:Chickens; D003059:Cocos; D004531:Eggs / Q000032:analysis; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D004376:Galactitol / Q000378:metabolism; D007106:Immune Sera; D008460:Meat / Q000032:analysis; D008722:Methods; D012475:Salmonella / Q000276:immunology / Q000302:isolation & purification*
D002250:Carbon Radioisotopes; D007106:Immune Sera; D004376:Galactitol