Evidence that 3-aminopicolinate stimulates glutamine metabolism by rat renal cortical tissue in vitro.
The hyperglycemic agent 3-aminopicolinate used at concentrations of 0.05 and 0.1 mM stimulates the removal of 5 mM glutamine. It also stimulates the accumulation of glutamate and the formation of ammonia, glucose and aspartate by isolated rat kidney-cortex tubules. These effects are consistent with a stimulation of glutaminase and an inhibition of phosphoenolpyruvate carboxy-kinase by this compound. Higher concentrations (0.5 and 1 mM) of 3-aminopicolinate fail to affect the removal of 5 mM glutamine, but greatly alter the fate of both glutamine carbon and amino nitrogen. Similar effects of 3-aminopicolinate are observed when glutamine is used at a near-physiological concentration.
D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D005972:Glutaminase / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism*; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D007684:Kidney Tubules / Q000378:metabolism; D008297:Male; D010848:Picolinic Acids / Q000494:pharmacology*; D051381:Rats
D010848:Picolinic Acids; D005973:Glutamine; C018451:3-aminopicolinic acid; D005972:Glutaminase