Synthesis of glycopeptides containing the amino acid sequence 17-23 of bovine pancreatic deoxyribonuclease.
2-Acetamido-3,4,6-tri-O-acetyl-1-N-[N-(benzyloxycarbonyl-L- seryl)-L-aspart-1-oyl-(p-nitrobenzyl ester)-4-oyl]-2-deoxy-beta-D-glucopyranosylamine, 2-acetamido-3,4,6-tri-O-acetyl-1-N-[N-(benzyloxycarbonyl-L-seryl)-L-aspart-1-oy l-(L-alanine methyl ester)-4-oyl]-2-deoxy-beta-D-glucopyranosylamine, and 2-acetamido-3,4,6-tri-O-acetyl-1-N-[N-benzyloxycarbonyl)-L-aspart-1-oyl-(L-alan yl-L-threonyl-L-leucyl-L-alanyl-L-serine p-nitrobenzyl ester)-4-oyl]-2-deoxy-beta-D-glucopyranosylamine (7), which span the amino acid sequence 17-23 of bovine pancreatic deoxyribonuclease A and contain a 2-acetamido-2-deoxy-D-glucose residue, were synthesized. On treatment with lithium hydroxide, the blocked glycohexapeptide 7 gave 2-acetamido-1-N-[N-(benzyloxycarbonyl)-L-aspart-1-oyl-(L-alanyl-L-threonyl-L-le ucyl-L-analyl-L-serine)-4-oyl]-2-deoxy-beta-D-glucopyranosylamine.
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003851:Deoxyribonucleases / Q000138:chemical synthesis*; D006020:Glycopeptides / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D008722:Methods; D010179:Pancreas / Q000201:enzymology*
D006020:Glycopeptides; D007202:Indicators and Reagents; D003851:Deoxyribonucleases