Frequency and diversity of RNA sequences in different cell types of the fungus Schizophyllum commune.
In the basidiomycete Schizophyllum commune an RNA complexity of 13,500 average-sized (1100 nucleotides) RNA sequences was found by means of RNA excess hybridization to complementary DNA. 600 abundant poly(A)-containing RNA sequences were present in about 100 copies per cell, whereas the rare 13,000 poly(A)-containing RNA sequences were present in about 5 copies per cell on average. Homologous and heterologous cDNA hybridizations to poly(A)-rich RNAs revealed some quantitative, but no significant qualitative differences between the monokaryotic and the dikaryotic cell type. Heterologous hybridization and subsequent hybridizations of remaining single-stranded cDNA to poly(A)-rich RNAs from both cell types showed qualitative differences amounting to 2-3% of the rare sequences in the two cell types. However, from the results of similar hybridizations with total RNAs, i.e. including non-polyadenylated RNA, it could be calculated that the RNA sequences in monokaryons and dikaryons are at least 99% the same. Identity of RNA sequences in monokaryons and dikaryons was also inferred by comparison of the patterns of polypeptides synthesized by translation of total RNA in cell-free systems.
D000363:Agaricales / Q000235:genetics*; D001483:Base Sequence; D002474:Cell-Free System; D004271:DNA, Fungal; D004586:Electrophoresis; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000302:isolation & purification; D014176:Protein Biosynthesis; D012331:RNA, Fungal; D012567:Schizophyllum / Q000235:genetics*
D004271:DNA, Fungal; D012331:RNA, Fungal; D011061:Poly A