Purification of opioid peptides from molluscan ganglia.
1. Acid extracts from pedal ganglia of Mytilus edulis was fractionated by high-pressure liquid chromatography (HPLC). 2. Components were recovered which had the same retention times as met- and leu-enkephalin. 3. When tested in an in vitro assay, these components inhibited dopamine-stimulated adenylate cyclase activity in a naloxone-reversible manner. 4. These results suggest that molluscan neural tissue contains opioid peptides similar, perhaps identical, to the mammalian enkephalins.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D049872:Bivalvia / Q000032:analysis*; D018492:Dopamine Antagonists; D004723:Endorphins / Q000302:isolation & purification* / Q000494:pharmacology; D005724:Ganglia / Q000032:analysis*
D018492:Dopamine Antagonists; D004723:Endorphins; D000262:Adenylyl Cyclases