Cytomorphological study of cultured mammalian cells treated with benzaldehyde, catechol and their derivatives with special reference to inhibition of DNA synthesis.
Cytotoxicity and cytomorphological changes induced in cultured mammalian cells by the application of benzaldehyde, pyrocatechol and their derivatives were examined. Among the compounds tested, 3,4-dihydroxybenzaldehyde (protocatechualdehyde) and 1,2-dihydroxybenzene (pyrocatechol) produced cytomorphological changes characteristic of specific inhibitors of DNA synthesis in V79 cells and EJ-1 cells. However, they did not induce such changes in HeLa cells. 2,3-Dihydroxybenzaldehyde and catecholamines did not produce such characteristic changes in V79 cells. The cytological effects of protocatechualdehyde and pyrocatechol were confirmed by a study on the incorporation of 3H-precursors of DNA, RNA and protein into 3 cell lines.
D000818:Animals; D001547:Benzaldehydes / Q000494:pharmacology*; D002396:Catechols / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D001749:Urinary Bladder Neoplasms / Q000378:metabolism; D014594:Uterine Neoplasms / Q000378:metabolism
D001547:Benzaldehydes; D002396:Catechols; C005581:protocatechualdehyde; D004247:DNA