Alterations in lipid metabolism induced by 12-O-tetradecanoylphorbol-13-acetate in differentiating human myeloid leukemia cells.
Several aspects of lipid metabolism were evaluated in differentiating human myeloid leukemia (HL-60) cells after treatment with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate. Modifications accompanying the phorbol ester-induced differentiation include an increase in the incorporation of acetate into free fatty acids and neutral lipids, an increase in the amount of neutral glycerolipids, and a selective incorporation of long-chain fatty alcohols into triacylglycerols and ether-linked alkyldiacylglycerols. Additionally, an enhanced stimulation of phospholipid metabolism, as measured by the incorporation of 32P and labeled precursors of the polar head groups, could be detected within 4 hr after treatment of cells with the tumor promoter. 4-O-Methyltetradecanoylphorbol-13-acetate, an analog with poor tumor-promoting activity, failed to elicit any activity on phospholipid metabolism.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D004075:Diglycerides / Q000378:metabolism; D006801:Humans; D007942:Leukemia, Experimental / Q000378:metabolism*; D050356:Lipid Metabolism; D009363:Neoplasm Proteins / Q000378:metabolism; D010704:Phorbols / Q000494:pharmacology*; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010716:Phosphatidylinositols / Q000032:analysis; D010743:Phospholipids / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014280:Triglycerides / Q000378:metabolism
D004075:Diglycerides; D009363:Neoplasm Proteins; D010704:Phorbols; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D010743:Phospholipids; D014280:Triglycerides; D013755:Tetradecanoylphorbol Acetate