Two-dimensional electrophoresis of human-CHO cell hybrids containing human chromosome 11.
Two-dimensional electrophoresis-electrofocusing with polyacrylamide gels is described in which the pattern of peptide spots obtained from hybrid cells of CHO containing the single human chromosome 11 are compared with those from the hybrid which has undergone reversion so as to lose this chromosome. At least eight distinct spots unique to the cell containing chromosome 11 have been located on the electrophoretogram. Experiments are described demonstrating applicability of the method to hybrids containing specific deletion mutants of chromosome 11, an approach which makes possible regional mapping of the loci responsible for specific peptides. The methodology appears applicable to study of gene expression under the influence of hormones and other agents and to the comparison of normal and disease situations.
D000818:Animals; D002906:Chromosomes, Human, 6-12 and X; D006224:Cricetinae; D003412:Cricetulus; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism*; D010053:Ovary; D010641:Phenotype; D011506:Proteins / Q000378:metabolism*
D011506:Proteins