Chemical characterization of porcine blood serum components with A and SLA activity.
The porcine A blood group substance is found in the serum as a lipid and, additionally, in certain animals, as a glycoprotein. Swine lymphocyte antigens (SLA) occur in the serum only as glycoproteins. Heat treatment of the solid residue obtained by lipid extraction yielded a water-soluble fraction with low protein content, high A activity, but no SLA activity. Poly(glycosyl)ceramides with SLA activity do not occur in the serum; poly(glycosyl)ceramides with A activity cannot be excluded. Desialylation of protein fractions has no effect on A and SLA activity. Both A and SLA activities of protein fractions are stable to mild alkaline hydrolysis thus indicating N-glycosidic carbohydrate-peptide linkages.
D000017:ABO Blood-Group System; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D008214:Lymphocytes / Q000276:immunology*; D013552:Swine / Q000097:blood*; D013556:Swine, Miniature
D000017:ABO Blood-Group System; D000954:Antigens, Surface