Complexes of nitracrine with DNA. Stoichiometry of binding.
In the presence of sulfhydryl compounds, an anticancer drug 1-nitro-9-(3-N,N-dimethylaminopropylamino) acridine (nitracrine, Ledakrin) forms irreversible complexes of decreased template activity with DNA. Stoichiometry of the complexes was estimated using the drug labelled with 14C in the acridine ring or in the propyl chain and with 3H in the acridine ring. Up to 50 irreversibly bound 14C-nitracrine molecules were found per 10(3) nucleotides of calf thymus DNA in the presence of dithiothreitol (DTT). Considerably lower binding observed using 3H-labelled drug, particularly when the complexes were formed in the presence of mercaptoethanol (ME) indicates that substitution of tritium atoms occurred during the reaction. Relationship between stoichiometry and template activity in RNA synthesis in vitro system of the complexes was estimated in the paper.
D000166:Acridines; D000609:Aminoacridines / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000032:analysis*; D004229:Dithiothreitol; D008623:Mercaptoethanol; D009564:Nitracrine / Q000032:analysis*
D000166:Acridines; D000609:Aminoacridines; D008623:Mercaptoethanol; D009564:Nitracrine; D004247:DNA; D004229:Dithiothreitol