A sensitive new method of "ex vivo" platelet deposition.
In 1978, an "in vivo" quantitative method of platelet aggregation based on the increment of weight of a rabbit tendon when superfused with flowing blood (3 ml/min) derived from a carotid artery of a cat and reentering the contralateral jugular vein was reported. TO increase the sensitivity of the method, we labeled platelets with indium-111 and reinjected them after two hours; then, with a gamma counter, we quantitated the 111In-labeled platelets deposited on the superfused rabbit tendon. Results of the radioactivity method and of the weight method were compared. We found that the 111In-labeling of platelets was more precise and reproducible method, rendering possible the use of a small amount of blood without need for reentry into the venous system.
D000818:Animals; D002415:Cats; D007204:Indium; D008722:Methods; D008855:Microscopy, Electron, Scanning; D010974:Platelet Aggregation; D010976:Platelet Count; D011868:Radioisotopes; D012044:Regression Analysis; D013710:Tendons / Q000648:ultrastructure; D013997:Time Factors
D011868:Radioisotopes; D007204:Indium