title: Effect of blood derived monocytes on the promotion of in vitro erythropoietic colony growth in human bone marrow cultures.
abstract: The effect of monocytes on the promotion of erythropoietic burst formation in human bone marrow methylcellulose cultures was studied in a burst-promoting activity (BPA)-poor system. Irradiated Ficoll-Isopaque separated blood mononuclear cells stimulated BFU-E growth proportionally to the number of irradiated cells added. Monocyte-depleted mononuclear cells stimulated as well as total mononuclear cells. Monocyte-concentrates stimulated when added at low concentrations. Monocyte-depleted mononuclear cells, although stimulatory at higher concentrations, did not stimulate at the concentrations used for monocyte-concentrates.
mesh_terms: D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004920:Erythropoiesis; D006133:Growth Substances / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D009000:Monocytes / Q000502:physiology*
chemical_list: D006133:Growth Substances