Fluorescence microscopy used in conjunction with horseradish peroxidase localization and electron microscopy for studying sympathetic nuclei of the rat spinal cord.
After application of horseradish peroxidase (HRP) to the transected cervical sympathetic trunk, labeled sympathetic neurons were seen in the intermediolateral nucleus (IML), central autonomic nucleus (CAN) and intercalated nucleus of rat spinal cords. As studied by glyoxylic acid-induced histofluorescence microscopy (FM), catecholaminergic (CA) terminals were most densely packed in the IML. There were 60 +/- 2 CA varicosities per 2,200 mu2 area in 20 mu thick sections through the IML. A combined FM/HRP method confirmed that preganglionic sympathetic neurons are heavily innervated by CA terminals. CA boutons were tagged with 5-hydroxydopamine for EM identification, and in the IML, 56% of CA boutons were seen to make synaptic contacts as compared to 60% in the CAN. It seems likely that virtually all CA boutons may form synapses but serial sections through boutons were not studied. It is inferred that preganglionic sympathetic neurons of both IML and CAN are served by CA inputs, and electron microscopy has revealed that most or all of these CA terminals transmit their signals via synapses; which may permit more precise structural sorting than non-synaptic transmission.
D000320:Adrenergic Fibers / Q000648:ultrastructure; D000818:Animals; D002395:Catecholamines / Q000378:metabolism; D005728:Ganglia, Sympathetic / Q000033:anatomy & histology*; D006735:Horseradish Peroxidase; D008297:Male; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009410:Nerve Degeneration; D009412:Nerve Fibers / Q000648:ultrastructure; D009474:Neurons / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000033:anatomy & histology*; D013569:Synapses / Q000648:ultrastructure
D002395:Catecholamines; D006735:Horseradish Peroxidase