title: Routine cryopreservation of ruminant nematode larvae.
abstract: Optimal conditions for cryopreservation of nematode larvae have been established using Nematospiroides dubius. Ruminant nematode larvae were infective up to three and a half years after freezing if introduced by laporotomy (intestinal nematodes) or by oral challenge (abomasal nematodes). The advantages of cryopreservation over normal storage of larvae are discussed.
mesh_terms: D000723:Ancylostomatoidea / Q000502:physiology; D000818:Animals; D002417:Cattle / Q000469:parasitology; D005615:Freezing; D009348:Nematoda / Q000502:physiology*; D011309:Preservation, Biological / Q000662:veterinary*; D051381:Rats; D012417:Rumen / Q000469:parasitology*; D012756:Sheep / Q000469:parasitology