Assessment study on the use of the low angle laser light scattering technique for the estimation of molecular weight of the polypeptide forming a complex with sodium dodecyl sulfate.
Molecular weights of the protein moieties of several complexes formed between sodium dodecyl sulfate and protein polypeptides of known molecular weights could be correctly estimated by the low angle laser light scattering technique (Kaye, W., Havlik, A.J. and MacDaniel, J.B. (1971) Polymer Lett. 9, 695-699). Since the surfactant can solubilize membrane proteins indiscriminately, the technique seems to be promising for the unique determination of molecular weights of membrane proteins.
D000818:Animals; D002417:Cattle; D007834:Lasers; D008722:Methods; D008970:Molecular Weight; D010455:Peptides; D011485:Protein Binding; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate
D010455:Peptides; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate