title: Changes in the concentrations of certain biochemical parameters in the peripheral blood during exercise and restitution after bloodletting.
abstract: The reported investigations were carried out in 18 men aged 19 to 23 years in whom 400 ml of whole blood was removed. On the day before bloodletting, one hour and 24 hours after it the studied men carried out a 10 minute exercise on a Monark cycle ergometer at a workload raising the heart rate to 170/min. Before the exercise, immediately after it and in the 30th minute of restitution venous blood samples were taken for determinations of the concentrations of total protein, albumins, free fatty acids, glucose, lactate and pyruvate, and the activity of lactic dehydrogenase, alanine aminotransferase and aspartate aminotransferase. During that time the acid-base equilibrium was determined in capillary blood. After bloodletting the concentrations of albumins, total protein and free fatty acids were decreased parallelly to haematocrit value decrease (p less than 0.05) and glucose concentration increased slightly (p less than 0.05). Enzyme activity was decreased slightly (p greater than 0.05). The partial oxygen pressure decreased, that of carbon dioxide increased, and hydrogen ion concentration rose. These changes were more pronounced after 24 hours than 1 hour after bloodletting. After submaximal exercise and in the 30th minute of restitution as well as 1 and 24 hours after bloodletting the changes in the concentrations of the biochemical parameters, enzyme activity and acid-base equilibrium were similar as after bloodletting.
mesh_terms: D000136:Acid-Base Equilibrium; D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis; D001815:Bloodletting; D002196:Capillaries; D004798:Enzymes / Q000097:blood; D005230:Fatty Acids, Nonesterified / Q000097:blood; D006801:Humans; D007773:Lactates / Q000097:blood; D008297:Male; D008660:Metabolism; D005082:Physical Exertion; D011773:Pyruvates / Q000097:blood; D012709:Serum Albumin / Q000032:analysis
chemical_list: D001786:Blood Glucose; D001798:Blood Proteins; D004798:Enzymes; D005230:Fatty Acids, Nonesterified; D007773:Lactates; D011773:Pyruvates; D012709:Serum Albumin