[Drug sensitivity test of cultured human cancer cells--assay for microcolonies inhibition test].
The sensitivity of anti-cancer drugs against cultured human lung cancer cells and the first cultured xenograft tumor has been measured by the microcolonies inhibition test in microplates (Falcon, Micro Test II). The results obtained were as follows; 1). The drug sensitivity of cancer cells has differed in each case. The established cancer cell lines of the same cell type and the same growth speed have also showed a different sensitivity. Therefore, it suggests that the measurement of drug sensitivity of cultured human cancer cells is usefull for the clinical application. 2). The drug sensitivity of cancer cells has been elevated when they changed their character during the passage cultures. On the other hand, the successful culture of cancer cells has been elevated when they were purified with discontinuous density gradients and a hypotonization. 3). In future, further efforts to develop a better medium for the colonies inhibition test, i. e., a conditioned medium containing the specific growth factor for the colony of cancer cells are imperative.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004351:Drug Resistance; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D051379:Mice
D000970:Antineoplastic Agents