[Action of x-rays and neuraminidase on pinocytotic activity and microtubule-microfilament system of Ehrlich ascites tumor cells in monolayer culture (author's transl)].
Monolayer cultures of Ehrlich ascites tumor cells in exponential growth phase were treated with X-rays and neuraminidase alone or in combination. A radiation dose of 2 Gy (200 rd) and higher effected a significant inhibition of DNA synthesis and cell proliferation. Neuraminidase treatment in addition to irradiation did not modify the growth-inhibitory irradiation effect. Cells pretreated for 0.5 to 1.0 hours with neuraminidase (in Earle's salt solution) and cultured thereafter (4h) in serum-containing growth medium exhibited an enhanced development of the microtubule-microfilament-system. Morphometric analysis of microtubules on electronmicroscopic sections revealed a nearly 4-fold increased number in neuraminidase treated cells while the average length of microtubules was similar to controls. Pinocytosis as measured by the ultrastructural uptake of ferritin appeared to be increased following neuraminidase treatment. A connection between neuraminic acid containing components (glycoproteins or glycolipids?) of the cell membrane or cell surface, on the one hand, and the cytoskeleton and the endocytotic process of these tumor cells, on the other hand, is suggested.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000532:radiotherapy* / Q000648:ultrastructure; D003599:Cytoskeleton / Q000187:drug effects* / Q000528:radiation effects; D008870:Microtubules / Q000187:drug effects* / Q000528:radiation effects; D009439:Neuraminidase / Q000494:pharmacology*; D010873:Pinocytosis / Q000187:drug effects* / Q000528:radiation effects; D011879:Radiotherapy Dosage
D009439:Neuraminidase
Einwirkung von Röntgenstrahlen und Neuraminidase auf Pinozytoseaktivität und Mikrotubulus-Mikrofilament-System von Ehrlich-Aszites-Tumorzellen in Monolayerkultur.