Effects of anemia and hypertransfusion on neonatal marrow and splenic erythrocytic colony-forming units in vitro.
Employing the plasma clot culture system, the effects of anemia or plethora on 10-day-old neonatal rat bone marrow and splenic erythrocytic colony-forming units (CFU-E) were examined. Anemia induced by phenylhydrazine resulted in a decreased CFU-E population in the bone marrow and dramatically increased CFU-E numbers in the spleen. Transfusion-induced plethora increased CFU-E numbers in both the marrow and the spleen. These in vitro findings lend support to studies performed in vivo and further suggest that regulation of erythropoiesis in the neonatal rat differs from that observed in the adult.
D000740:Anemia / Q000473:pathology; D000818:Animals; D000831:Animals, Newborn; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004912:Erythrocytes / Q000166:cytology*; D004920:Erythropoiesis; D004921:Erythropoietin / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D010659:Phenylhydrazines / Q000009:adverse effects; D051381:Rats; D012156:Reticulocytes / Q000166:cytology; D013154:Spleen / Q000166:cytology*
D010659:Phenylhydrazines; C030299:phenylhydrazine; D004921:Erythropoietin