title: Inability of mesoderm cells to locomote on the modified free surface of epithelial cell sheets in vitro.
abstract: Previous work has shown that when chick embryo mesoderm tissue is seeded onto the free, dorsal surface of established sheets of embryonic epithelial endoblast, the former penetrates the latter and spreads on the underlying artificial substratum. In this work, the surface charge on the epithelial sheet has been altered, prior to seeding the mesoderm, to ascertain whether such a change could alter the behavior of the mesoderm with respect to the free surface of the epithelium. Charge alteration was accomplished using the polycations, poly-L-lysine. Surface charge characteristics were examined ultrastructurally using cationized and anionized ferritin. Results showed that although surface charge changes were detectable, there was no difference in the behavior of the mesoderm with respect to the endoblast. Neuraminidase did not detectably affect the epithelial surface charge. These results are consistent with the view that changes in substratum surface charge are not necessarily correlated with changes in adhesiveness.
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002450:Cell Communication; D002462:Cell Membrane; D002465:Cell Movement; D002642:Chick Embryo; D004847:Epithelial Cells; D004848:Epithelium / Q000196:embryology*; D005293:Ferritins / Q000494:pharmacology; D066298:In Vitro Techniques; D008648:Mesoderm / Q000166:cytology*; D011107:Polylysine / Q000494:pharmacology; D013499:Surface Properties; D013997:Time Factors
chemical_list: D011107:Polylysine; D005293:Ferritins