Urea polyacrylamide gel electrophoresis of PCMB precipitate as a sensitive test for the detection of the unstable hemoglobin subunit.
A sensitive test for the detection of the abnormal subunit of unstable hemoglobins is described. The unstable hemoglobin subunit was selectively precipitated by paramercuribenzoic acid treatment of the carboxy hemolysate, and the precipitate was studied by urea polyacrylamide gel electrophoresis for globin composition. The test enabled the detection of an electrophoretically silent, alpha-chain unstable variant comprising only 1% of total hemoglobin. Other applications included the demonstration of Hb Köln in a cord blood, and the detection and purification of very slightly unstable hemoglobin variants.
D011232:Chemical Precipitation; D002729:Chloromercuribenzoates; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004899:Erythroblastosis, Fetal / Q000175:diagnosis; D005260:Female; D006454:Hemoglobins / Q000032:analysis*; D006461:Hemolysis; D006801:Humans; D011247:Pregnancy; D020245:p-Chloromercuribenzoic Acid
D002729:Chloromercuribenzoates; D006454:Hemoglobins; D020245:p-Chloromercuribenzoic Acid