Immune transfer using spleen fragments.
The adequacy of spleen slices for the purpose of adoptive immune transfer was evaluated in rodents. Transfers using spleen cell suspensions and spleen slices were conducted in parallel from hyperimmune donors to non-immune intact and irradiated syngeneic recipients. It was demonstrated that the level of immune responsiveness attained with transfer of spleen slices was at least equal to and most often greater than that achieved with transfer of spleen cell suspensions.
D000818:Animals; D000917:Antibody Formation; D000920:Antibody-Dependent Cell Cytotoxicity; D007114:Immunization; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D051381:Rats; D013154:Spleen / Q000276:immunology*; D014185:Transplantation, Isogeneic
D007074:Immunoglobulin G; D007075:Immunoglobulin M