Physiological requirements for ciliary reactivation of bracken fern spermatozoids.
Physiological parameters affecting reactivated ciliary beat in spermatozoids of braken fern (Pteridium aquilinum) were studied using a Triton/glycerol permeabilized cell model system. Reactivation frequencies of polylysine-tethered cells equalled in vivo rates at neutral pH. Frequency was dependent on ATP and Mg2+ concentration, and reactivation was inhibited by millimolar or greater free calcium. Reactivation was reversibly inhibited by micromolar concentrations of sodium ortho-vanadate, while intact cells were not affected by millimolar levels of the inhibitor. This is the first characterization of in vitro ciliary beat in a non-algal plant cell and demonstrates that the nucleotide and ionic requirements for reactivation of bracken cilia are similar to those of other systems.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002923:Cilia / Q000187:drug effects / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008854:Microscopy, Electron; D009068:Movement / Q000187:drug effects; D059828:Plant Cells; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D014639:Vanadium / Q000494:pharmacology
D014639:Vanadium; D000255:Adenosine Triphosphate; D008274:Magnesium; D002118:Calcium