The composition and optical rotary dispersion of bovine aqueous humor.
The concentration of over 20 biochemicals in bovine aqueous humor was determined by conventional means, and the contribution of each to the observed optical rotation was estimated. Of those studied, only glucose and the total proteins made a significant contribution. However, other unidentified chemicals apparently also contribute to the optical activity since it was not possible to reconstruct the actual aqueous humor optical rotation based on the chemicals included. Alternative experimental approaches that may yield more complete results are discussed. With further work, optical rotation might be used as the basis of a noninvasive sensor for measuring the glucose concentration of aqueous humor in the intact eye.
D000818:Animals; D001082:Aqueous Humor / Q000032:analysis*; D002417:Cattle; D005947:Glucose / Q000032:analysis; D009905:Optical Rotatory Dispersion
D005947:Glucose