In vitro and in vivo evaluation of the tissue-to-blood partition coefficient for physiological pharmacokinetic models.
An important parameter used in physiologically based pharmacokinetic models is the partition coefficient (Kp), which is defined as the ratio of tissue drug concentration to the concentration of drug in the emergent venous blood of the tissue. Since Kp is governed by reversible binding to protein and other constituents in blood and tissue, an attempt was made here to estimate the Kp values for a model drug ethoxybenzamide (EB) by means of in vitro binding studies and to compare these Kp values to those obtained from in vivo kinetic parameters observed following the administration of EB by two different routes, i.e., i.v. bolus injection and constant rate infusion. The Kp values obtained by using these three different methods were in reasonably good agreement suggesting that binding data obtained in vitro can successfully be used to estimate in vivo distribution.
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008954:Models, Biological; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D012457:Salicylamides / Q000378:metabolism; D013696:Temperature
D001798:Blood Proteins; D004364:Pharmaceutical Preparations; D012457:Salicylamides; C018935:ethenzamide