Association of initiation factor eIF-2 with a rapidly sedimenting fraction from Ehrlich ascites-tumour cells.
To determine the subcellular distribution of initiation-factor eIF-2 activity, Ehrlich ascites-cell homogenates were fractionated to give (a) a rapidly sedimenting fraction, (b) a microsomal fraction and (c) post-microsomal supernatant. The first two fractions were washed in 0.5 m-KCl to render the associated protein-synthesis factor soluble. As much as 60% of the total recoverable eIF-2 was obtained from the rapidly sedimenting material.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D015852:Eukaryotic Initiation Factor-2; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D010448:Peptide Initiation Factors / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl; D013347:Subcellular Fractions / Q000378:metabolism
D015852:Eukaryotic Initiation Factor-2; D010448:Peptide Initiation Factors; D011506:Proteins; D012346:RNA, Transfer, Amino Acyl; C025207:eIF-2 Met-tRNA(f)(met) GDPCP; D006160:Guanosine Triphosphate; D012343:RNA, Transfer