[A method for the determination of liver alkaline phosphatase in feces and their activity in intra- and extrahepatic cholestasis (author's transl)].
Using precipitating antibodies to human liver alkaline phosphatase (L-AP), a quantitative method is evaluated for the determination of L-AP-activity in feces. Normal controls (n = 42) exhibit a logarithmic normal distribution. Patients with intrahepatic cholestasis (n = 36) demonstrate an increase, patients with extrahepatic cholestasis (n = 23) a decrease of the fecal L-AP-activity. Variations of fecal L-AP-activities due to a varying water content of the stools could be excluded by simultaneous determination of intestinal AP-activity. Prae- and postoperative determinations in 12 patients with extrahepatic cholestasis yielded an increase of L-AP-activity after reconstructive surgery. In intrahepatic cholestasis, high fecal L-AP-activities are found together with relative low total AP-activities in the serum whereas in extrahepatic cholestasis low fecal L-AP-activities are associated with relative high total AP-activities in the serum.
D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D000906:Antibodies; D002779:Cholestasis / Q000201:enzymology; D002780:Cholestasis, Intrahepatic / Q000201:enzymology; D005243:Feces / Q000032:analysis*; D006801:Humans; D008099:Liver / Q000201:enzymology*; D011233:Precipitin Tests; D011817:Rabbits / Q000276:immunology
D000906:Antibodies; D000469:Alkaline Phosphatase
Eine Methode zur Bestimmung der alkalischen Leberphosphatase im Stuhl und ihre Aktivität bei intra- und extrahepatischem Verschlussikterus.