Spontaneous metastasis of cells of the human prostate carcinoma cell line PC-3 in athymic nude mice.
Spontaneous metastasis and extensive invasiveness were observed in athymic nude mice injected with human prostatic carcinoma cells of the PC-3 line or heterotransplants of nude mouse supported PC-3 tumor. In 3 experimental series, 60, 63 and 50 per cent of the nude mice receiving subcutaneous inoculations of PC-3 cells or tumor heterotransplants developed 1 or more lymphatic tumor metastases. Examination of metaphase-arrested cells recovered from the metastatic sites confirmed the tumor origin as human in each case. Cells recovered from 1 nude mouse supported subline, MPC-3-10, frequently exhibited double minute chromosomes in addition to the typical PC-3 chromosomal profile. These observations provide the foundation for a study of the relationship between prostate carcinoma cell characteristics and lymphatic metastasis in the nude mouse.
D000818:Animals; D002277:Carcinoma / Q000473:pathology*; D002460:Cell Line; D005260:Female; D006801:Humans; D007621:Karyotyping; D008207:Lymphatic Metastasis / Q000473:pathology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology; D011471:Prostatic Neoplasms / Q000473:pathology*; D014183:Transplantation, Heterologous