Lack of effect of zona antibody on the development of mouse embryos in vivo and in vitro.
The effects of purified antibody (IgG) to mechanically isolated or detergent-solubilized zonae pellucidae on the development and implantation of mouse embryos were studied in vitro by culture and in utero by transfer of treated embryos. The proportion of 8-celled embryos treated with zona antibody that developed into blastocysts and attached to the culture dish in vitro were not significantly different from those obtained after treatment of embryos with IgG from normal rabbit serum and antiserum raised against mouse liver and kidney. Pregnancy rate, mean number of implantations and live fetuses, and fetal sex ratio after transfer of zona antibody treated embryos were not significantly different from the values obtained after transfer of embryos treated with control serum IgG. It was concluded that treatment with zona antibody did not inhibit the development and implantation of mouse embryos in vitro or in vivo.
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D046508:Culture Techniques; D010064:Embryo Implantation; D004624:Embryo Transfer; D005260:Female; D007074:Immunoglobulin G / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D010063:Ovum / Q000502:physiology*; D011247:Pregnancy; D015044:Zona Pellucida / Q000276:immunology / Q000502:physiology*
D007074:Immunoglobulin G