Interactions between bovine cornea proteoglycans and collagen.
Two types of proteoglycan subunits were obtained from bovine cornea, the first mainly composed of proteochondroitin sulphate and the second of proteokeratan sulphate. These two fractions can be obtained from the tissue as an aggregate, and are able to recombine each other after separation, to re-form the original structure. In order to investigate collagen-proteoglycan interactions, type-I collagen was isolated from bovine cornea by pepsin digestion followed by 3.5% (w/v) NaCl precipitation, and was then linked to CNBr-activated Sepharose 4B. Two identical columns were prepared, the first filled with collagen coupled to Sepharose 4B, the second with free Sepharose 4B. The two proteoglycan subunits and the aggregate were chromatographed on the two gels under the same conditions; the elution profiles showed that both the aggregate and the proteochondroitin sulphate subunit are retarded by the collagen coupled to Sepharose. No interaction, however, occurred when proteokeratan sulphate subunit was run through the columns. Chondroitinase digestion of the proteoglycan samples confirmed that chondroitin sulphate chains are mainly responsible for the interaction with collagen; their removal, in fact, completely abolishes any differences between the chromatographic behaviour on the collagen-Sepharose and the control columns.
D000818:Animals; D002417:Cattle; D002810:Chondroitinases and Chondroitin Lyases; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D003094:Collagen / Q000302:isolation & purification* / Q000378:metabolism; D003315:Cornea / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000302:isolation & purification; D046911:Macromolecular Substances; D010206:Papain; D011509:Proteoglycans / Q000302:isolation & purification* / Q000378:metabolism
D006025:Glycosaminoglycans; D046911:Macromolecular Substances; D011509:Proteoglycans; D003094:Collagen; D010206:Papain; D002810:Chondroitinases and Chondroitin Lyases