Specificity in the hydrolysis of N-acyl-L-phenylalanine 4-nitroanilides by chymotrypsin.
The affinity of N-acyl-L-phenylalanine 4-nitroanilides for chymotrypsin is enhanced as the hydrophobicity of non-amino acid residues in the P2-position of the substrates increases, whereas Kcat remains nearly constant. On the other hand, if alanine or leucine is in the P2-position Kcat increases with decreasing KM.
D000813:Anilides; D000818:Animals; D002417:Cattle; D002918:Chymotrypsin / Q000378:metabolism*; D007700:Kinetics; D010649:Phenylalanine / Q000031:analogs & derivatives*; D013379:Substrate Specificity
D000813:Anilides; D010649:Phenylalanine; D002918:Chymotrypsin