Fluorescent observations on the stress fiber system of neural crest cells after con A treatment. A simple, quick, fluorescent method.
Concanavalin A (Con A), 25 microgram/ml in the tetrameric form, inhibits migration and differentiation of cultured amphibian neural crest. Affected cells loose their angulated morphology and become rounded. A correlation exists between the lectin treatment and the overall cellular and stress fiber morphology. We describe a quick, simple method for disclosing the stress fiber system using fluorescence microscopy after staining with 1% alcoholic eosin.
D000557:Ambystoma; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002464:Cell Migration Inhibition; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D003599:Cytoskeleton / Q000648:ultrastructure*; D008856:Microscopy, Fluorescence / Q000379:methods; D009432:Neural Crest / Q000166:cytology* / Q000187:drug effects; D011952:Receptors, Concanavalin A / Q000378:metabolism
D011952:Receptors, Concanavalin A; D003208:Concanavalin A