title: A Histochemical study of the coronary vasculature in euthyroid and hyperthyroid rats.
abstract: Coronary arteries and arterioles in the left and right ventricles from normal and hyperthyroid rats were examined histochemically to determine and to compare their metabolic activities. The test animals were made hyperthyroid by administration of desiccated thyroid for 8-10 weeks. Using histochemical techniques, selected enzymes and components of key metabolic pathways were examined. These pathways included an evaluation of aerobic (oxidative phosphorylation, Kreb's cycle and respiratory chain) and anaerobic metabolic capacity, hexose-monophosphate shunt activity, amounts of deoxyribonucleic and ribonucleic acids present and activity of beta-oxidation of fatty acids. Our results indicate that normal coronary arteriolar metabolism is predominantly aerobic. The findings also suggest a reduction in aerobic metabolism with an accompanying increase in anaerobic potential in the hyperthyroid coronary arterioles. Thus, during thyrotoxicosis, the coronary arterioles may partially shift from aerobic to anaerobic metabolism. Moreover, in both the normal and thyrotoxic rat heart, the coronary microvasculature appears quite stable with little cell proliferation. In contrast, both the control and hyperthyroid rat coronary arteries appear to utilize primarily anaerobic pathways, while the control and hyperthyroid myocardium seem highly dependent upon aerobic metabolism. The tremendous reduction in glucose-6-phosphate dehydrogenase activity in hyperthyroid, when compared to normal coronary arteries and some larger arterioles, implies a reduced capacity for nucleic acid and protein synthesis in the test animals.
mesh_terms: D000818:Animals; D003331:Coronary Vessels / Q000032:analysis* / Q000201:enzymology; D006003:Glycogen / Q000032:analysis; D006651:Histocytochemistry; D006980:Hyperthyroidism / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000032:analysis; D009696:Nucleic Acids / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D009696:Nucleic Acids; D006003:Glycogen