Morphological aspects of manganese-bilirubin induced cholestasis.
Small doses of manganese or bilirubin administered alone to rats produce no morphologic alterations in the hepatocytes. However, combination of both treatments produced a rapid and severe decrease in bile flow that was paralleled by the appearance and aggravation of the cholestatic lesion: bile canaliculi progressively lost their microvilli; vacuolization occurred in the pericanalicular area and subsequently in the cytoplasm. When bile flow returned to normal, or when cholestasis was prevented by an injection of sulfobromophthalein or a pretreatment with phalloidin, no cholestatic signs were observed. A close correlation appears to exist between the presence of morphologic alterations and the decrease of bile flow in manganese-bilirubin cholestasis.
D000818:Animals; D001646:Bile / Q000187:drug effects; D001663:Bilirubin / Q000494:pharmacology*; D002780:Cholestasis, Intrahepatic / Q000139:chemically induced / Q000473:pathology*; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D008345:Manganese / Q000494:pharmacology*; D010590:Phalloidine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D010590:Phalloidine; D008345:Manganese; D001663:Bilirubin