[DNA replication in adult and aged rat tissues].
The incorporation of single-stranded DNA replication intermediates (Okazaki fragments) into double-strand polymerized DNA and the chain growth of these intermediates in adult and aged rat tissues with low and high mitotic activities were studied. These processes were found to proceed in a similar manner independently of the tissue mitotic activity. However, in tissues with a low mitotic activity of adult and aged rats the incorporation of Okazaki fragments into double-stranded DNA was decelerated as compared to those with a high mitotic activity. Since the mitotic activity and ageing were shown to have similar effects on the rate of stabilization of replicating DNA secondary structure, it may be assumed that the factors responsible for regulation of DNA replication rate are altered with ageing.
D000375:Aging; D000818:Animals; D004247:DNA / Q000302:isolation & purification; D004261:DNA Replication; D008297:Male; D008940:Mitotic Index; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009928:Organ Specificity; D051381:Rats
C022262:Okazaki fragments; D004247:DNA
Nekotorye svoĭstva replikatsii DNK v tkaniakh vzroslykh i starykh krys.