The suppression of tumorigenicity in human X mouse cell hybrids. II. The relationship between tumorigenicity and parameters of transformation in vitro.
We have studied the expression of transformation-related parameters in tertiary hybrid clones from an apparently transformed human cell strain (ME) and a malignant mouse cell line (A9). The derivation of these hybrid clones, chromosome analysis, and tumorigenic potential have been reported elsewhere (1). In the present work, the serum sensitivity, saturation density, cloning efficiency on plastic surfaces and in semisolid medium, survival in aggregate form, concanavalin A agglutinability, sugar uptake, and secretion of plasminogen activator in 4 tertiary hybrid clones and in both parent cells have been determined. While, in general, the extent to which these transformation-related traits were expressed in the hybrid clones closely resembled the malignant A9 parent cell, all hybrids displayed partial to complete suppression of tumorigenicity. These results suggest no apparent relationship between the extent of quality of transformation and tumorigenicity in these cell hybrids.
D000371:Agglutination; D000818:Animals; D001769:Blood; D002448:Cell Adhesion; D002449:Cell Aggregation; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D003208:Concanavalin A / Q000494:pharmacology; D003847:Deoxyglucose / Q000378:metabolism; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D009374:Neoplasms, Experimental / Q000209:etiology*; D010960:Plasminogen Activators / Q000378:metabolism
D003208:Concanavalin A; D003847:Deoxyglucose; D010960:Plasminogen Activators