The screening of alpha- and beta-globin chain variants with isoelectric focusing.
A simple isoelectric focusing technique is presented for the screening of alpha- and beta-globin chain variants on polyacrylamide gel in the presence of 8 M urea. No detergent was used. Variants focused at discrete pH values, depending on the charge variation (-2, -1, 0, +1 or +2) introduced by the mutation. A linear relationship was evidenced between the charge variation and the shift of the apparent isoelectric point of the variants. The practical interest of this procedure is illustrated.
D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D005914:Globins / Q000032:analysis / Q000145:classification / Q000235:genetics*; D006455:Hemoglobins, Abnormal / Q000032:analysis / Q000235:genetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D007525:Isoelectric Focusing; D008297:Male
D006455:Hemoglobins, Abnormal; D005914:Globins