Inhibitory effect of oxidized glutathione on heart ornithine decarboxylase activity.
Cardiac ODC was prepared from rats after the injection of isoproterenol and was partially purified by thiol-affinity chromatography. This enzymatic preparation incubated with GSSG was inactivated in a concentration dependent process. The inactivated enzyme could be reactivated by the addition of various reducing compounds, including GSH. Kinetic studies on GSSG-inactivated ODC revealed that the enzyme was inhibited by a partially-non competitive mechanism.
D000818:Animals; D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors*; D005978:Glutathione / Q000031:analogs & derivatives* / Q000494:pharmacology; D019803:Glutathione Disulfide; D006321:Heart / Q000187:drug effects; D007545:Isoproterenol / Q000494:pharmacology; D009206:Myocardium / Q000201:enzymology*; D065108:Ornithine Decarboxylase Inhibitors; D051381:Rats; D013997:Time Factors
D065108:Ornithine Decarboxylase Inhibitors; D002262:Carboxy-Lyases; D005978:Glutathione; D007545:Isoproterenol; D019803:Glutathione Disulfide