The isolation of rat lung cells for the purpose of studying drug metabolism.
A method was developed for the isolation of viable cells from rat lungs. The cells were a heterogeneous population, which maintained a high percentage viability for up to 3 hr on incubation at 37 degrees. Reduced cofactor levels (NADH, NADPH) decreased on incubation, but ATP remained constant. The cells were active in the metabolism of the two xenobiotics studied. A model compound, 7-ethoxycoumarin, was O-deethylated and subsequently conjugated with glucuronic acid and sulphate, whilst a pharmaceutical agent, N-acetylcysteine, was de-acetylated.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D003374:Coumarins / Q000378:metabolism; D066298:In Vitro Techniques; D008168:Lung / Q000166:cytology* / Q000378:metabolism; D008297:Male; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D003374:Coumarins; D004364:Pharmaceutical Preparations; D009243:NAD; D009249:NADP; D000255:Adenosine Triphosphate