Rat serum sickness: possible role of inflammatory mediators allowing deposition of immune complexes in the glomerular basement membrane.
Serum sickness was induced in rats by a modification of previously described methods avoiding i.v. administration of the antigen. All the animals developed a progressive disease characterized by an initial pattern of deposition of IC in the mesangium followed by the appearance of GBM deposits. This change in the deposition of IC was associated with the onset of massive proteinuria and a fall in the titre of precipitating antibodies. Simultaneously, specific desensitization of platelets for a rat PAF could be demonstrated and platelet aggregates were seen in the glomeruli. The presence of homocytotropic IgGa anti-ovalbumin antibodies in rat sera during the induction of the disease was demonstrated by 2 hr PCA. Accordingly, this antibody together with the antigen ovalbumin induced the release of histamine from peritoneal mast cells, suggesting that a similar mechanism might occur in vivo during the induction of the disease. Rat PAF and beta glucuronidase could be obtained from peritoneal macrophages under similar conditions to those required for the release of histamine. The data support a role for inflammatory mediators in the increase in vascular permeability needed for the deposition of IC in the GBM and provide evidence for a new role of macrophages and PMNs in glomerular pathology in contributing to an increase in permeability of GBM.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000936:Antigen-Antibody Complex / Q000378:metabolism*; D001485:Basement Membrane / Q000276:immunology; D004195:Disease Models, Animal; D006632:Histamine / Q000276:immunology*; D007678:Kidney Glomerulus / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology; D010972:Platelet Activating Factor / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D012713:Serum Sickness / Q000276:immunology*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D010972:Platelet Activating Factor; D006632:Histamine; D010047:Ovalbumin