[Effect of cytochalasin B on the distribution of concanavalin A receptors of the surfaces of normal mouse fibroblasts].
Normal mouse fibroblasts are spread over the glass in the cultural medium containing cytochalasin B (CB). The cultures were incubated with concanavalin A (Con A), which binds some surface components. Con A-labeled surface receptors were visualized by the indirect immunogluorescent method. When living cultures were incubated with Con A, small patches of Con A-bound receptors were formed over the surface. 5 hours after seeding, the control cells formed plates of the lamelloplasm, whose external edge was active, with pseudopodia being formed along it. The patches of receptors were cleared selectively, from the surface of psedopodia and of the lamelloplasm, to the central parts of the cell surface. In contrast, CB-treated cells, during spreading, formed thin ribbon-like pseudopodia. The properties of these CB-treated pseudopodia differed from those formed by control cells; the former were unable to remove patches from the pseudopodial surface. The effect of CB was reversible: 30 minutes after the removal of CB, small parts of the lamelloplasm appeared, from which patches of receptors were removed. These experimental data suggest that CB-treated cells may form pseudopodia with a deficient structure and modified functional properties.
D000818:Animals; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D051379:Mice; D011952:Receptors, Concanavalin A / Q000187:drug effects*
D011952:Receptors, Concanavalin A; D003571:Cytochalasin B
Vliianie tsitokhalazina B na raspredelenie retseptorov konkanavalina A na poverkhnosti normal'nykh fibroblastov myshi.