[Serum amino acid determination after different blood processing and storage methods].
Blood samples of four volunteers were prepared for amino acid analysis in different ways using 39 methods. The result was that after initial deproteinisation of the blood samples different storage temperatures ranging from -18 degrees C to -78 degrees C and different sorting periods from 2 to 6 months did no influence the amino acid spectra. Storage at -4 degrees C did not show a clear result, however slightly decreased glutamine levels. It is possible to allow blood samples to stand at room temperature for further processing for at least 60 minutes, without adverse influence on the analytical result.


CONCLUSIONS
If it is impossible to carry out a quantitative amino acid analysis immediately after blood withdrawal, the blood sample can be stored for several months at not more than -18 degrees C provided that within about 90 minutes after blood withdrawal centrifugation and deproteinisation has been carried out.
D000328:Adult; D000596:Amino Acids / Q000097:blood*; D001793:Blood Preservation / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D013048:Specimen Handling / Q000379:methods; D013696:Temperature
D000596:Amino Acids
Aminosäurenbestimmungen im Serum nach unterschiedlicher Aufarbeitung und Lagerung der Blutproben.