The high mobility group proteins and transcribed nucleosomes.
Monomer nucleosomes released from nuclei during brief micrococcal nuclease digestions are enriched in transcribed sequences (Bloom and Anderson, 1978). These nucleosomes are depleted in H1 and enriched in three high mobility group proteins HMG14, HMG17 and another HMG-like protein. Analysis of such nucleosomes by polyacrylamide gel electrophoresis reveal that they are heterogenous. Similarly, monomer nucleosomes soluble in 0.1 M NaCl separate on polyacrylamide gels into mainly two types of particle, one of which has HMG14 and HMG17 bound. However, the DNA of the HMG-nucleosomes from chick erythrocytes is not enriched in globin sequences, suggesting that protein rearrangement may have occurred.
D000818:Animals; D002645:Chickens / Q000097:blood; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000378:metabolism; D009707:Nucleosomes / Q000378:metabolism*; D014158:Transcription, Genetic
D002868:Chromosomal Proteins, Non-Histone; D009707:Nucleosomes; D004247:DNA