[Kinetic parameters of a soluble purified intestinal fucosyl-transferase].
The soluble fucosyl-transferase of the rat small intestinal mucosa was purified by passing through a Sephadex G-100 column, then resolved in two isoenzymes F1 (pHi = 4,47) and F2 (pHi = 4,96) by isoelectric focusing. The use in the first step of DEAE-cellulose instead of Sephadex G-100 leads to another component F3 (pHi = 8,70). Kinetic parameters (KM et Vm) for the three isoenzymes are given. The enzymatic mechanism seems to be a bi-bi random type for the three isoenzymes, and F3 appears as the most active species.
D000818:Animals; D003636:DEAE-Cellulose; D005647:Fucosyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D006602:Hexosyltransferases / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000201:enzymology*; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000302:isolation & purification; D007700:Kinetics; D051381:Rats; D012995:Solubility
D007527:Isoenzymes; D003636:DEAE-Cellulose; D005647:Fucosyltransferases; D006602:Hexosyltransferases
Paramètres cinétiques d'une fucosyl-transférase intestinale soluble purifiée.