[Inhibitory action of divalent copper compounds on cumene hydroperoxide oxidative demethylation of N,N-dimethylaniline by cytochrome P-450].
The inhibitory action of divalent copper compounds on hydroperoxide-dependent oxidative demethylation of N,N-demethylaniline involving rabbit liver microsomes and highly purified cytochrome P-450 has been studied. CuCl2 is a non-competitive inhibitor, whereas copper tyrosine and lysine complexes are characterized by a mixed type inhibition. The inhibitory action of copper complexes is based on a decrease of cumene hydroperoxide concentration. The reaction results in formation of RO and RO2 radicals destroying cytochrome P-450 CuCl2 (0,001 M) also destroys cytochrome P-450 in the absence of cumene hydroperoxide; the destruction process is characterized by two phases with different rate constants. The nature of the inhibitory action of CuCl2 on N,N-demethylaniline oxidation by hydroperoxides is discussed.
D000814:Aniline Compounds; D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology*; D003300:Copper / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D007700:Kinetics; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D010545:Peroxides / Q000494:pharmacology*; D011817:Rabbits
D000814:Aniline Compounds; D001555:Benzene Derivatives; D010545:Peroxides; C015157:N,N-dimethylaniline; D003300:Copper; D003577:Cytochrome P-450 Enzyme System; C007164:cumene hydroperoxide
Ingibiruiushchee deĭstvie soedineniĭ medi na okislitel'noe demetilirovanie dimetilanilina gidroperekis'iu kumila pri uchastii tsitokhorma P-450.