Production of monoclonal antibodies against hepatitis B surface antigen (HBsAg) by somatic cell hybrids.
Hybridomas secreting anti-HBs were produced by fusion of either adw or ayw HBsAg primed mouse spleen cells with either P3 X63 Ag8 or P3 NSI 1 Ag4 1 mouse myeloma cell lines. Individual anti-HBs secreting clones were isolated by limiting dilution procedures, and six cell lines have been established, namely, BX182, BX259, BX248, CN324, DN283, and DN296. Progenies of each cell line were derived from a single clone obtained from three subclonings of six anti-HBs positive initial fusion colonies. Clones were passaged in tissue culture and as tumors in syngeneic mice for upwards of six months. Anti-HBs of each line showed characteristic reactivity (detection) patterns in radioimmunoassay using different antigen subtype solid phases followed by either 125I-HBsAg or 125I-goat anti-mouse IgG probe. The specificity of the anti-HBs from each clone for the subdeterminants of HBsAg was identified by their reaction with 125I-HBsAg ligands of several subtypes in a radioimmunoprecipitation assay. Four types of reaction were identified and correlated to the conventional serological subtyping definitions; they were anti-HBs/a (BX259 and CN324), anti-HBs/d (BX182), and possibly anti-HBs/w (BX248 and DN296) and anti-HBs/y (DN283). These monoclonal antibodies will be important for the elucidation of the antigenic structure of native HBsAg and will provide valuable reagents for both antigen detection and subtyping.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002459:Cell Fusion; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000096:biosynthesis* / Q000145:classification; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006822:Hybrid Cells / Q000276:immunology*; D051379:Mice; D009101:Multiple Myeloma; D013154:Spleen / Q000166:cytology
D000914:Antibodies, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens