A method for determining the three-dimensional form of active flagella, using two-colour darkground illumination.
Using darkground illumination, and placing colour filters over half the field, one can make a single, highly resolved, colour micrograph of a filamentous object (such as a mammalian sperm tail) from which it is possible to get a complete and unambiguous three-dimensional reconstruction.
D000818:Animals; D006224:Cricetinae; D008029:Lighting; D008297:Male; D008853:Microscopy / Q000379:methods*; D010784:Photomicrography / Q000379:methods*; D051381:Rats; D013082:Sperm Tail / Q000648:ultrastructure*; D013094:Spermatozoa / Q000648:ultrastructure*