Novel vesicular extrusions during cell spreading.
During the late stages of cell spreading in vitro, the cells extrude a vesicular material into the medium. This phenomenon was observed in human glia and glioma cells as well as in human diploid fibroblasts MRC-5 and WI-38 cells. This extrusion of vesicular material is inhibited by cytochalasin-B and colcemid suggesting the involvement of microfilaments and microtubules and the active nature of this event. It appears that the cells may be excreting damaged surface components by a mechanism similar to patching, capping and endocytosis.
D002460:Cell Line; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology; D003703:Demecolcine / Q000494:pharmacology; D005347:Fibroblasts / Q000502:physiology*; D005910:Glioma / Q000503:physiopathology*; D006801:Humans; D008870:Microtubules / Q000502:physiology; D009457:Neuroglia / Q000502:physiology*; D009940:Organoids / Q000502:physiology
D003571:Cytochalasin B; D003703:Demecolcine