title: Levels of glutamate, aspartate, GABA, and taurine in different regions of the cerebellum after x-irradiation-induced neuronal loss.
abstract: The levels of glutamate (Glu), aspartate (Asp), gamma-amino-n-butyric acid (GABA), and taurine (Tau) were determined in the cortex, molecular layer, and deep nuclei of cerebella of adult rats exposed to X-irradiation at 12-15 days following birth (to prevent the acquisition of late-forming granule cells; 12-15x group) and 8-15 days following birth (to prevent the acquisition of granule and stellate cells; 8-15x group). Also, the levels of the four amino acids were measured in the crude synaptosomal fraction (P2) isolated from the whole cerebella of the control, 12-15x, and 8-15x groups. The level of Glu was significantly decreased by (1) 6-20% in the cerebellar cortex; (2) 15-20% in the molecular layer; and (3) 25-50% in the P2 fraction of the X-irradiated groups relative to control values. The content of Glu in the deep nuclei was not changed by X-irradiation treatment. Regional levels of Asp were unchanged by X-irradiation, while its level in P2 decreased by 15-30% after treatment. The levels of GABA and Tau in the molecular layer, deep nuclei, or P2 were not changed in the experimental groups. However, there was a 15% increase in the levels of GABA and Tau in the cerebellar cortex of the 8-15x group relative to control values. The data support the proposed role of glutamate as the excitatory transmitter released from the cerebellar granule cells but are inconclusive regarding a transmitter role for either Tau or GABA from cerebellar stellate cells.
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism / Q000528:radiation effects*; D005971:Glutamates / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000528:radiation effects*; D051381:Rats; D013654:Taurine / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D005971:Glutamates; D013654:Taurine; D001224:Aspartic Acid; D005680:gamma-Aminobutyric Acid