Migration of leukocytes into filters coated homogeneously with immune complexes, antigens, lectins or tripeptides.
Cellulose nitrate filters were incubated in solutions of albumin, a chemotactically active tripeptide (f-Met-Leu-Phe), immune complexes or lectins and afterwards washed with buffer. They showed a dose-dependent increased leukocyte migration, when tested in typical Boyden chambers in comparison to filters treated only with buffer. The tripeptide, the immune complexes and the lectins were stimulatory at very low concentrations and acted inhibitory at high concentrations. Treating filters with formaldehyde or glutardialdehyde had no clear stimulatory effect. These findings extend earlier observations obtained with casein. They show that cells move very effectively on solid substrata in the absence of concentration gradients.
D000818:Animals; D000936:Antigen-Antibody Complex; D002465:Cell Movement; D002630:Chemotactic Factors / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte; D003208:Concanavalin A / Q000494:pharmacology; D006098:Granulocytes; D006168:Guinea Pigs; D007074:Immunoglobulin G; D037102:Lectins / Q000494:pharmacology; D008851:Micropore Filters; D009842:Oligopeptides / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D012709:Serum Albumin / Q000494:pharmacology
D000936:Antigen-Antibody Complex; D002630:Chemotactic Factors; D007074:Immunoglobulin G; D037102:Lectins; D009842:Oligopeptides; D010835:Phytohemagglutinins; D012709:Serum Albumin; D003208:Concanavalin A