A cell cycle study of the effects of Con A on synchronized mouse embryo fibroblasts: arrest at two specific stages of the cycle and dissociation between uptake of thymidine and DNA synthesis.
We have examined the effects of 50 microgram ml-1 of Con A added to synchronized mouse embryo fibroblasts at different times during the cell cycle. We found that Con A caused arrest of growth not solely by preventing G1-G0 cells from entering the S-phase but also by exerting a G2 block. We also found that Con A, which prevented commencement of S-phase, did not arrest cells already in S from reaching the G2 stage but inhibited the S-phase associated process of thymidine uptake. The inhibition was greater when the Con A receptors were extensively clustered.
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007399:Interphase / Q000187:drug effects; D008766:Methylmannosides / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D011952:Receptors, Concanavalin A / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D008766:Methylmannosides; D011952:Receptors, Concanavalin A; D003208:Concanavalin A; D004247:DNA; D013936:Thymidine