A transcriptionally enriched chromatin fraction released by a Mg++ -dependent endogenous nuclease from rat liver.
When rat liver nuclei are subjected to endogenous nuclease digestion in the presence of 5 mM Mg++, they release a chromatin fraction containing DNA of well defined fragment lengths and DNA-dependent RNA polymerases. The DNA is enriched 4.4-fold for sequences complementary to cDNA prepared to cytoplasmic poly-A-containing RNA when compared with whole cell DNA. This method of obtaining a transcriptionally enriched chromatin fraction is compared with other reported nuclease methods.
D000818:Animals; D002843:Chromatin / Q000235:genetics*; D004247:DNA / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology*; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects
D002843:Chromatin; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D003851:Deoxyribonucleases; D008274:Magnesium