The suppressive effect of hepatic 9,000 x G supernatant fraction from 3-methylcholanthrene-treated rats on the mutagenic activity of 2-acetylaminofluorene.
Hepatic 9,000 x g supernatant (S9) fraction from 3-methylcholanthrene (MC)-treated rats showed a significantly low activity in the in vitro conversion of 2-acetylaminofluorene (AAF) to mutagen(s), while S9 fraction from untreated or phenobarbital (PB)-treated rats showed a remarkable activity. The S9 fraction from MC-treated rats actually suppressed the PB-S9 mediated AAF mutagenicity. Since the suppressive effect of MC-S9 was lost by the addition of 10(-4) M alpha-naphthoflavone (alpha-NF), rat specific cytochrome P-448 may be responsible for this suppressive effect.
D015073:2-Acetylaminofluorene / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D000818:Animals; D001571:Benzoflavones / Q000494:pharmacology*; D004357:Drug Synergism; D005419:Flavonoids / Q000494:pharmacology*; D008099:Liver / Q000166:cytology*; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology*; D009153:Mutagens; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D013347:Subcellular Fractions / Q000502:physiology*
D001571:Benzoflavones; D005419:Flavonoids; D009153:Mutagens; D008748:Methylcholanthrene; C011512:alpha-naphthoflavone; D015073:2-Acetylaminofluorene; D010634:Phenobarbital