title: Surface interaction in vitro between Sertoli cells and germ cells at different stages of spermatogenesis.
abstract: The presence of surface-recognition mechanisms between somatic and germ cells of the seminiferous epithelium has been studied in the rat by an assay in vitro based on the ability of homogeneous populations of spermatogenic cells, at specific differentiative stages, to adhere to monolayers of cultured Sertoli cells. The results show that germ cells adhere specifically to Sertoli cells and that the adhesion is dependent on the differentiative stage of the germ cells. Pachytene spermatocytes show the highest ability to adhere and form typical junctional specializations with the underlying Sertoli cells, while round spermatids adhere much less to the substrate. The possible regulative role of a somatic cell-germ interaction is discussed.
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D002469:Cell Separation; D008297:Male; D051381:Rats; D012670:Seminiferous Epithelium / Q000166:cytology*; D012708:Sertoli Cells / Q000502:physiology*; D013091:Spermatogenesis; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*; D013737:Testis / Q000166:cytology*