Experimental allergic encephalomyelitis. Characterization of serum factors causing demyelination and swelling of myelin.
Serum factors in rabbits with white matter-induced experimental allergic encephalomyelitis (WM-EAE) were studied with respect to their role in demyelination in vitro in organotypic central nervous system (CNS) tissue cultures and in vivo in the myelinated retina of the rabbit eye. By absorption with staphylococcal protein A, IgG was quantitatively separated from the other serum proteins. No IgG was demonstrable in the absorbed IgG-depleted sera by Ouchterlony double diffusion, immunoelectrophoresis and SDS-polyacrylamide gel electrophoresis. Both the IgG-depleted WM-EAE sera and the IgG fractions had complement-dependent demyelinating activity on CNS cultures, and both contained immunoglobulin binding to myelin and oligodendroglia of the cultures, as demonstrated by an immunoperoxidase technique. However, only the purified IgG fractions in the absence of complement induced swelling of myelin and proliferation of oligodendroglial processes with redundant myelin in tissue cultures. The IgG-depleted complement-inactivated WM-EAE sera produced no morphological changes. In the rabbit eye model, antibody-dependent cell-mediated demyelination was observed only with the IgG fractions but not with the IgG-depleted EAE sera. No oligodendroglial proliferation occurred. These studies demonstrate for the first time that in CNS cultures, non-IgG immunoglobulins as well as IgG mediate complement-dependent demyelination and that these bind to myelin and oligodendrocytes, whereas only IgG causes myelin swelling and oligodendrocyte proliferation.
D000818:Animals; D003165:Complement System Proteins / Q000276:immunology; D046508:Culture Techniques; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology; D007074:Immunoglobulin G / Q000276:immunology; D007136:Immunoglobulins / Q000276:immunology*; D008854:Microscopy, Electron; D009186:Myelin Sheath / Q000276:immunology* / Q000648:ultrastructure; D009836:Oligodendroglia / Q000276:immunology; D011817:Rabbits
D007074:Immunoglobulin G; D007136:Immunoglobulins; D003165:Complement System Proteins