Effect of genetic differences on K+-induced metabolic changes in allogeneic murine brain cortical cells incubated in vitro.
Stimulation of oxygen uptake by brain cortex cells with high K+ concentration is one of model reactions characteristic for the functional activity in vitro. The present study was undertaken to clarify the initial findings of the near abolition of K+-induced stimulation of oxygen uptake by mixed brain cortex cells which originate from genetically different mice. Increase in K+-stimulated oxygen consumption by mixed brain cortex cells from H-2 compatible mice (C3H/Cbi/Bom and CBA/J strains) reached 150%. K+-induced oxygen uptake by mixed cells from H-2 incompatible mice (A/Ph and C3H/Cbi/Bom) reached 104%. K+-stimulated respiration by allogeneic cells from congenic lines with H-2 differential loci was also lowered. K+-induced breakdown of ATP and creatine phosphate was similar in both allogeneic cells (to 72% and 64%, respectively) and syngeneic controls (to 72% and 61%, respectively). Factors involved in the demonstration of allogeneic interactions of brain cortex cells were tested. K+-mediated metabolic activity in allogeneic brain cortex cells was dependent on strong antigenic differences, cell-cell contacts and optimal amount of interacting cells. It, therefore, seems that genetic differences such as in H-2 complex linked alloantigens may participate in alterations of some metabolic processes of the brain function.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D003470:Culture Media; D008285:Major Histocompatibility Complex; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D010101:Oxygen Consumption / Q000187:drug effects; D010725:Phosphocreatine / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology*
D003470:Culture Media; D010725:Phosphocreatine; D011189:Potassium Chloride; D000255:Adenosine Triphosphate