title: [Isoenzymes of glucose-6-phosphate dehydrogenase from Aspergillus oryzae (Ahlburg) (author's transl)].
abstract: Two forms (I and II) of Glucose-6-phosphate dehydrogenase (D-Glucose-6-phosphate: NADP+ oxidoreductase E.C. 1.1.1.49) were isolated from mycelium of Aspergillus oryzae grown on glucose as sole carbon source, through ammonium sulfate fractionation, followed by ion-exchange chromatograhy. The Km values for both G6P and NADP+ were very similar, but the Vmax was nearly twofold for form II. The two isoenzymes were inhibited by NADPH competitively with NADH+, and the Ki value was minimal for isoenzymatic form I. The ratio Ki/Km was 2.5 for isoenzyme I and 7.5 for isoenzyme II. The two isoenzymatic forms were inhibited by energetic metabolites (ATP, ADP and PEP), the greater effect was caused by ATP.
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D001230:Aspergillus / Q000201:enzymology*; D001236:Aspergillus oryzae / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D009249:NADP / Q000494:pharmacology
chemical_list: D007527:Isoenzymes; D009249:NADP; D000255:Adenosine Triphosphate; D005954:Glucosephosphate Dehydrogenase
vernacular_title: Isoenzimas de la glucosa-6-fosfato deshidrogenasa de Asperigillus oryzae (Ahlburg).