Gas chromatographic determination of phentolamine (Regitine) in human plasma and urine.
A method for the determination of unconjugated phentolamine at concentrations down to 5 ng/ml in human plasma, and of free and total (free plus conjugated) phentolamine down to 25 ng/ml in urine is described. After addition of 2-[N-(p-chlorophenyl)-N-(m-hydroxyphenyl)-aminomethyl]-2-imidazoline as internal standard, both compounds are extracted into benzene-ethyl acetate (1:1, v/v) at pH 10, transferred into an acidic aqueous solution and back-extracted at pH 10 into benzene-ethyl acetate. They are then derivatized with N-heptafluorobutyrylimidazole. The derivatives are determined by gas chromatography using a 63Ni electron-capture detector. In urine, total (free plus conjugated) phentolamine is determined after enzymatic hydrolysis. The technique was applied for the study of the plasma concentrations and urinary elimination after oral administration to man.
D001192:Arylsulfatases; D002849:Chromatography, Gas; D005966:Glucuronidase; D006801:Humans; D008297:Male; D008832:Microchemistry; D010646:Phentolamine / Q000097:blood* / Q000652:urine
D001192:Arylsulfatases; D005966:Glucuronidase; D010646:Phentolamine