Induction of chromosomal aberrations and 8-azaguanine-resistant mutations by aryldialkyltriazenes in cultured mammalian cells.
Inducibility of chromosomal aberrations, cell survival, and mutation to 8-azaguanine (8AG) resistance in cultured V79 cells by 1-phenyl-3,3-dimethyltriazene (PDMT), 1-phenyl-3,3-diethyltriazene (PDET), and 1-(pyridyl-3)-3,3-diethyltriazene (PyDET) were examined with or without metabolic activation. Chromosomal aberrations were induced in a dose-dependent manner by all 3 triazenes in a direct treatment for 24 h. Chromosomal aberrations were also induced by PDMT with metabolic activation system for 3 h, and little differences in the incidences were observed compared with those obtained by a direct treatment. All 3 triazenes were slightly mutagenic in a direct treatment for 24 h. In metabolic activation experiments, however, PDMT and PyDET were highly mutagenic. The mutagenicity, when compared with the cytotoxicity, was significantly higher in a metabolic activation system than in a direct treatment.
D000818:Animals; D001375:Azaguanine / Q000494:pharmacology; D002460:Cell Line; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D008168:Lung; D009154:Mutation; D014226:Triazenes / Q000494:pharmacology*
D014226:Triazenes; D001375:Azaguanine