Plaque assay of Heliothis zea baculovirus employing a mixed agarose overlay.
The nuclear polyhedrosis virus of Heliothis zea has been titrated in Heliothis zea cells by the plaque method, using 1 percent mixed agarose containing a mixture of Seakem and Ultra pure agarose. Visible plaques, formed 8 days postinfection, ranged in diameter from 0.5 to 2 mm. Dose-response experiments indicated that a single particle initiated the formation of a plaque. The titration of Heliothis zea baculovirus by the newly described plaque method provides an accurate technique for the determination of virus concentration.
D000818:Animals; D002460:Cell Line; D007304:Insect Viruses / Q000254:growth & development*; D009036:Moths; D012685:Sepharose; D010948:Viral Plaque Assay / Q000379:methods*
D012685:Sepharose