Gas chromatographic analysis of therapeutic concentrations of maprotiline in serum, using flame-ionization detection.
For the measurement of the tetracyclic antidepressant maprotiline in human serum, a gas chromatographic method with flame-ionization detection has been developed. The assay specifications obtained are as follows: a precision (C.V) of 3.5-6.4%, and a relative recovery of 97-109% using amitriptyline as internal standard. The sensitivity of the assay from serum was 40 nmol/l. The applicability of the method has been shown by measuring steady-state serum levels of five inpatients. The steady-state serum levels of maprotiline given at a daily dosage of 75 mg varied from 272 to 570 nmol/l.
D000873:Anthracenes / Q000097:blood*; D000928:Antidepressive Agents / Q000097:blood; D002849:Chromatography, Gas / Q000379:methods*; D005410:Flame Ionization / Q000379:methods*; D006801:Humans; D008376:Maprotiline / Q000008:administration & dosage / Q000097:blood*
D000873:Anthracenes; D000928:Antidepressive Agents; D008376:Maprotiline