Cellular and molecular heterogeneity of H-2Kk antigens.
Up to 70% of mouse spleen cells expressing H-2Kk alloantigens reacted with the monoclonal anti-H-2Kk antibody 11-4.1 in an indirect rosetting assay. When the cells that rosetted with the monoclonal antibody 11-4.1 were removed by density centrifugation, the residual unreactive cells reacted with the polyclonal anti-H-2Kk alloantiserum D23 but not with the monoclonal antibody 11-4.1 indicating the existence of two cel populations. In sequential immunoprecipitation experiments, the glycoprotein fraction of NP40 extracts from H-2Kk cells, after removal of antigens reacting with the monoclonal antibody 11-4.1 still contained structures reactive with the alloantiserum. Therefore, there are at least two antigenically distinct H-2Kk molecules which are differentially expressed on two subpopulations of spleen cells.
D000818:Animals; D000918:Antibody Specificity; D002999:Clone Cells / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D006183:H-2 Antigens / Q000032:analysis*; D006822:Hybrid Cells / Q000276:immunology; D007518:Isoantibodies; D051379:Mice; D012397:Rosette Formation; D013154:Spleen / Q000276:immunology
D006023:Glycoproteins; D006183:H-2 Antigens; D007518:Isoantibodies