Specific cell binding using staphylococcal protein A magnetic microspheres.
Protein A, a protein derived from Staphylococcus aureus, was incorporated into the matrix of magnetic albumin microspheres. Because staphylococcal protein A binds most subclasses of immunoglobulin G through their Fc portions, immunoglobulin may be rapidly bound to microspheres without chemical coupling agents or a diamino-heptane spacer group. Microspheres so prepared bind specifically to a given cell type when incubated in vitro with a heterogeneous cell population. The use of these microspheres as a drug carrier capable of cellular specificity as well as their ability to isolate homogeneous cell populations rapidly is discussed.
D000818:Animals; D002645:Chickens; D007136:Immunoglobulins / Q000378:metabolism; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000378:metabolism; D008280:Magnetics; D008863:Microspheres; D012756:Sheep; D013205:Staphylococcal Protein A / Q000378:metabolism*
D007136:Immunoglobulins; D013205:Staphylococcal Protein A