Differences in rearrangements of H1 and H5 in chicken erythrocyte chromatin.
H1 can rearrange in chicken erythrocyte polynucleosomes in 80 mM NaCl buffers. These rearrangements have been studied by sedimentation analysis. H1 redistributes between polynucleosomes as well as between polynucleosomes and monosomes. In these rearrangements H1 molecules move to free DNA sites. In contrast to H1, the chicken erythrocyte specific lysine-rich histone H5 does not show any of these dynamic properties. This difference in mobility of H1 and H5 also manifests itself in the selective extraction of H1 from H1, H5 containing polynucleosomes by the cation-exchange resin AG 50W-X2 at 80 mM NaCl.
D000818:Animals; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D002645:Chickens; D002843:Chromatin / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis*; D006657:Histones / Q000097:blood* / Q000302:isolation & purification; D008970:Molecular Weight
D002843:Chromatin; D006657:Histones