title: Lack of deviation from Michaelis--Menten kinetics for pig heart fumarase.
abstract: Studies of steady-state kinetics of fumarase in the usual substrate-concentration range from 0.1 Km to 10 Km and in the high substrate-concentration range from 10 Km to 200 Km are described. The purpose is to investigate reports of substrate inhibition and oscillatory kinetics. In the normal substrate-concentration range, no deviations from hyperbolic kinetics were found, and in the extended concentration range, up to more than 200 times the Km value, no substrate inhibition was demonstrated. A discussion of the discrepancies between the mentioned reports of deviations from the hyperbolic kinetics and the present findings is given.
mesh_terms: D000818:Animals; D005649:Fumarate Hydratase / Q000378:metabolism*; D007700:Kinetics; D008293:Malates; D009206:Myocardium / Q000201:enzymology*; D013552:Swine
chemical_list: D008293:Malates; D005649:Fumarate Hydratase