A simple method for the preparation of phosphatidylcholine labelled at 2-acyl position.
Phosphatidylcholine with a labelled acyl group at 2 position was prepared enzymatically from lysophosphatidylcholine and labelled fatty acids. Under optimal conditions, over 90% of [5,6,8,9,11,12,14,15-3H] arachidonic acid, [9,10-3H] palmitic acids, [1-14C] linoleic acid or [9,10-3H] oleic acid was incorporated into the phosphatidylcholine, and the incorporation was quantitative up to 500 nmol of [1-14C] linoleic acid. Analysis of the labelled phosphatidylcholine formed indicated that radioactivity was incorporated exclusively at 2-acyl position.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D002250:Carbon Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D008041:Linoleic Acids / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D009829:Oleic Acids / Q000378:metabolism; D010169:Palmitic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D051381:Rats; D014316:Tritium
D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D008041:Linoleic Acids; D009829:Oleic Acids; D010169:Palmitic Acids; D010713:Phosphatidylcholines; D014316:Tritium