Immunological interference of the synthetic aromatase inhibitor 1,4,6-androstatriene-3,17-dione (ATD) and its metabolite(s) in the radioimmunoassay for testosterone.
Radioimmunoassay (RIA) for testosterone (T) in unchromatographed plasma extracts from ATD-treated rats gave spuriously high values for T. Cross-reaction and chromatographic studies subsequently showed that ATD and, to a much greater extent, its metabolite(s) were responsible for this overestimation. Celite column chromatography proved to be an effective way of separating T from ATD and its product(s) of metabolism.
D000733:Androstatrienes / Q000097:blood / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity; D002845:Chromatography; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D013739:Testosterone / Q000097:blood* / Q000276:immunology
D000733:Androstatrienes; C011658:androsta-1,4,6-triene-3,17-dione; D013739:Testosterone