[Effect of surface-active substances on hydroxylase activity of Tieghemella orchidis in a free and immobilized state].
The effect of nonionogenic surface-active substances (SAS) on hydroxylase activity of Tieghemella orchidis in free and polyacrylamide gel (PAAG) immobilized states was studied. It was shown that addition of SAS (1%) to the cultivation medium simultaneously with the inoculation stimulated the filamentous growth of the mycelium. It was also demonstrated that certain SAS (span-80/sorbitanemonooleate/and polyethylene glycol-600-monolaurate) affected beneficially the culture transformation activity. The hydroxylase activity also increased after mycelium preincubation with tweens (1%) and transformation in the presence of span-20 (sorbitane monolaurate) and span-80 (0.3%). The use of the latter substances during gel preincubation with immobilized cells led to the stabilization of transformation activity, increase in the hydroxylation rate, and a change in the yield of hydroxylated products to produce hydrocortisone.
D004800:Enzymes, Immobilized / Q000378:metabolism*; D007700:Kinetics; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D009090:Mucorales / Q000201:enzymology*; D013329:Structure-Activity Relationship; D013501:Surface-Active Agents / Q000494:pharmacology*
D004800:Enzymes, Immobilized; D013501:Surface-Active Agents; D006899:Mixed Function Oxygenases
Izuchenie deĭstviia poverkhnostno-aktivnykh veshchestv na gidroksilaznyiu aktivnost' kletok Tieghemella orchidis v svobodnom i immobilizovannom sostoianii.