[The phenomenon of double line of IgG in immunoelectrophoresis (author's transl)].
Immunoelectrophoretic studies of patients' sera occasionally reveal a parallel splitting of the IgG precipitation line if the test is performed with polyvalent antihuman antiserum directed not only against the Fc-part of the IgG molecule. This phenomenon has been studied in a patient with discoid lupus erythematosus and another patient with lymphadenopathy of unknown origin. Apparently, this finding was not due to a monoclonal gammopathy: In the cellulose membrane electrophoresis no extragradient could be observed and assays with antisera directed against free and bound light chains showed a regular pattern. The splitting of the IgG precipitate is much rather caused by polyclonal IgG with a high proportion of slowly diffusing heterogenous IgG-x, as has been found in studies using the immunoelectrophoresis as well as the analytical ultracentrifuge. It was not due to Fab fragments.
D005260:Female; D006801:Humans; D007119:Immunoblastic Lymphadenopathy / Q000276:immunology; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000032:analysis*; D008179:Lupus Erythematosus, Discoid / Q000276:immunology; D008297:Male; D010265:Paraproteinemias / Q000276:immunology*
D007074:Immunoglobulin G
Beitrag zum Phänomen von Doppellinien für IgG in der Immunoelektrophorese.