[Change of C19-21 steroids levels of maternal serum, amnion and cord plasma by high pressure liquid chromatography (HPLC) (author's transl)].
With normal pregnant and abnormal pregnant cases as the subjects steroids in the maternal serum at term and plasma cord were measured by HPLC and the following results were obtained. 1) The blood at term proved to contain cortisol 165.06 +/- 38.90 ng/ml, cortisone 85.97 +/- 16.40 ng/ml, progesterone 23.42 +/- 8.77 ng/ml and the blood at delivery to contain cortisol 574.68 +/- 156.76 ng/ml, cortisone 100.94 +/- 27.54 ng/ml and progesterone 41.14 +/- 18.31 ng/ml. Amnion contained cortisol 69.40 +/- 16.72 ng/ml, cortisone 26.11 +/- 9.13 ng/ml, progesterone 29.36 +/- 9.35 ng/ml, cord plasma contained cortisol 109.64 +/- 27.41 ng/ml, cortisone 373.32 +/- 88.60 ng/ml and progesterone 450.53 +/- 113.84 mg/ml. 2) The blood at delivery compared to that term showed especially higher lever of cortisol. Maternal serum showed the pattern of cortisol superiority of the corticoid, while cord plasma showed the pattern of cortisone superiority. 3) Significant correlation was observed between amnion and cord plasma in the gestagen and between amnion and maternal serum-cord plasma in corticoid. 4) In severe toxemia of pregnancy and pregnancy with diabetes mellitus some steroids showed significant low levels as compared to normal case. 5) In severe toxemia of pregnancy cortisone/cortisol ratio was irregular as compared to normal case.
D000653:Amniotic Fluid / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003348:Cortisone / Q000378:metabolism; D005260:Female; D005312:Fetal Blood / Q000378:metabolism*; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D011283:Pregnenes / Q000097:blood / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism; D014471:Umbilical Veins
D011283:Pregnenes; D013739:Testosterone; D011374:Progesterone; D003348:Cortisone; D006854:Hydrocortisone