Crystallization and preliminary X-ray data for chloroperoxidase.
The enzyme chloroperoxidase from the fungus Caldariomyces fumago has been crystallized in a form suitable for detailed three-dimensional x-ray structure determination. Crystals grown from polyethylene glycol solutions display high resolution diffraction patterns indicating the orthorhombic space group C2221: a = 151.1 (3), b = 57.9 (4), c = 102.7 (9) A, V = 9 X 10(5) A3. A volume to mass ratio Vm = 2.8 A3/dalton for 8 molecules in the unit cell and a measured density of 1.35 g/cm3 suggest that the asymmetric unit contains a single chloroperoxidase molecule.
D000818:Animals; D002711:Chloride Peroxidase / Q000302:isolation & purification; D003460:Crystallization; D003904:Mitosporic Fungi / Q000201:enzymology; D010544:Peroxidases / Q000302:isolation & purification; D011487:Protein Conformation; D014961:X-Ray Diffraction
D010544:Peroxidases; D002711:Chloride Peroxidase