Protection against cis-dichlorodiammine Pt (II) cytotoxicity in vitro by cysteamine.
EMT6/SF cells exposed to cis-dichlorodiammine Pt(II) (DDP) for 1 hr in the presence of 10 mM cysteamine (MEA) survived better than cells treated with DDP alone (DMF-3.1). MEA added to cultures after removal of DDP also protected, even when the two exposures were separated by as long as 4 hr. Protection by MEA was concentration dependent: no significant protection was observed below 1 mM and maximum protection required 5 mM. When cells were incubated with 10 mM MEA immediately after removal of DDP, survival increased as a function of the length of exposure to MEA, reaching a maximum at 2 hr.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002945:Cisplatin / Q000037:antagonists & inhibitors* / Q000633:toxicity; D003543:Cysteamine / Q000494:pharmacology*; D008325:Mammary Neoplasms, Experimental / Q000503:physiopathology; D013997:Time Factors
D003543:Cysteamine; D002945:Cisplatin