A cytochemical section-bioassay for thyrotropin.
A cytochemical section-bioassay of thyrotropin has been developed which retains the sensitivity of the earlier segment-assay (10(-4) microunits/ml) and allows a considerable increase in the rate at which these within-animal bioassays can be done. The index of precision (n=23) was 0.11 +/- 0.06; intra-assay variation was 1.3%; inter-assay variation was 10.9 +/- 6%. Fiducial limits (p = 0.95) ranged from 65-150% to 78-127%. The system is also capable of detecting thyroid-stimulating immunoglobulins.
D000626:Aminopeptidases / Q000378:metabolism; D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D005260:Female; D006168:Guinea Pigs; D006651:Histocytochemistry; D007986:Luteinizing Hormone; D008247:Lysosomes / Q000201:enzymology; D013972:Thyrotropin / Q000276:immunology / Q000494:pharmacology; D013997:Time Factors
D000906:Antibodies; D007986:Luteinizing Hormone; D013972:Thyrotropin; D000626:Aminopeptidases