title: [Microbiological assay method of cefadroxil in biological specimens (author's transl)].
abstract: A microbiological method for quantitative determination of cefadroxil in biological specimens is described. This method is essentially a cylinder-plate or a paper-disc method using Micrococcus luteus ATCC 9341 as the test organism grown in the tryptosoya broth added with 1.5% agar. Cefadroxil standard calibration curves are prepared in pooled human serum, moni-trol I or consera for the determination of serum level of human, and 0.1 M phosphate buffer (pH 6.0) to determine urine level. Cefadroxil in human serum and urine specimens can be measured by cylinder-plate method as low as 0.16 approximately 0.31 micrograms/ml and 0.08 approximately 0.16 micrograms/ml, respectively. Further, any active metabolites of cefadroxil were not detected on human and rat urine specimens by bioautography.
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002434:Cefadroxil; D002506:Cephalexin / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D006801:Humans; D008297:Male; D008837:Micrococcus / Q000187:drug effects; D051381:Rats
chemical_list: D002434:Cefadroxil; D002506:Cephalexin