Twenty-four to ninety-six-hour storage of human spermatozoa in test-yolk buffer.
The feasibility of a simple, inexpensive technique of short-term (24- to 96-hour) preservation of human spermatozoa in TEST-yolk buffer is demonstrated. Preservation of spermatozoa for up to 72 hours would require no addition of antibiotics. There is little loss of motility when semen is added to TEST-yolk buffer and kept in a standard refrigerator. Such semen would then be available for multiple inseminations during the periovulatory period. One objection to this procedure is that it is necessary to dilute semen 1:1 in TEST-yolk buffer, resulting in a 50% lower sperm density, and this may be objectionable when oligospermia is present. Results obtained in this study are in agreement with similar studies performed on domestic animals.
D000328:Adult; D000332:Aerobiosis; D000693:Anaerobiosis; D002021:Buffers; D006801:Humans; D008297:Male; D010406:Penicillins / Q000494:pharmacology; D011309:Preservation, Biological; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*; D013307:Streptomycin / Q000494:pharmacology; D013997:Time Factors
D002021:Buffers; D010406:Penicillins; D013307:Streptomycin