Isolation of adult canine venous endothelium for tissue culture.
In order to provide autologous adult endothelial cells for the production of cell-lined artificial vascular prostheses, we have developed a method for harvesting large numbers of cells with minimal contamination by other cellular types. In this technique, the vein is incubated in trypsin-EDTA solution followed by collagenase and the endothelial cells flushed off with a stream of culture medium. With care and appropriate timing, the endothelium can be selectively removed leaving the underlying basal lamina intact.
D000818:Animals; D002469:Cell Separation / Q000379:methods; D046508:Culture Techniques / Q000379:methods*; D004285:Dogs; D004727:Endothelium / Q000166:cytology; D007601:Jugular Veins / Q000166:cytology*