Glucocorticoid regulation of metallothionein-I mRNA synthesis in cultured mouse cells.
Eight mouse cell lines were tested for their ability to accumulate metallothionein-I mRNA in response to dexamethasone or cadmium; 5 respond to both inducers, 1 responds to cadmium only, and 2 lymphoid lines respond to neither inducer. The glucocorticoid response was analyzed further using the sarcoma cell line (S180). Metallothionein-I mRNA synthesis increases 5-fold within 1 h of dexamethasone addition and closely parallels the accumulation of nuclear glucocorticoid receptors. Both metallothionein-I mRNA levels and metallothionein synthesis increase coordinately in response to dexamethasone and reach a new steady state by 8 h that is 10-fold higher than control values. Induction of metallothionein-I mRNA appears to be independent of dexamethasone effects on zinc uptake and does not require concomitant protein synthesis.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002104:Cadmium / Q000494:pharmacology; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D007700:Kinetics; D008667:Metalloproteins / Q000235:genetics*; D008668:Metallothionein / Q000235:genetics*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics*; D012513:Sarcoma, Experimental; D014158:Transcription, Genetic / Q000187:drug effects*; D015032:Zinc / Q000378:metabolism
D008667:Metalloproteins; D012333:RNA, Messenger; D002104:Cadmium; D003907:Dexamethasone; D008668:Metallothionein; D015032:Zinc