A new histochemical method for demonstration of sulfhydryl groups.
This paper described a new histochemical method for the detection of sulfhydryl groups in tissue specimens using 2,6-dichloroquinone chloroimide G.R. (Merck) after the reduction of its chloride groups of sodium thiosulfate. Ths proposed mechanism of the reaction, the procedures and the histological applications are described.
D000818:Animals; D016227:Benzoquinones; D004284:Dogfish / Q000378:metabolism; D005088:Exocrine Glands / Q000032:analysis; D006651:Histocytochemistry / Q000379:methods*; D007097:Imines; D051379:Mice; D011077:Polychaeta / Q000032:analysis; D011809:Quinones; D051381:Rats; D012867:Skin / Q000032:analysis*; D013438:Sulfhydryl Compounds / Q000032:analysis*
D016227:Benzoquinones; D007097:Imines; D011809:Quinones; D013438:Sulfhydryl Compounds; C010358:N,2,6-trichloro-4-benzoquinone imine