Conjugation of triaziquinone to immunoglobulin G by a thiolation procedure catalyzed by 2-pyridinealdoxime methiodide.
Thiolation of immunoglobulin G (IgG) with DL-N-acetylhomocysteinethiolactone, catalyzed by 2-pyridinealdoxime methiodide, incorporated new sulfur groups into IgG. Triaziquinone was subsequently conjugated to the sulfur groups. Triaziquinone-IgG complex retained the alkylating activity of the drug and the immunological activity of the antibody. The conjugation procedure was inhibited by the thiol-blocking agent methyl methanethiolsulfonate.
D000477:Alkylating Agents / Q000494:pharmacology; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000918:Antibody Specificity; D055598:Chemical Phenomena; D002621:Chemistry; D006389:Hemagglutinins, Viral / Q000276:immunology; D007120:Immunochemistry; D007074:Immunoglobulin G / Q000276:immunology; D009980:Influenza A virus / Q000276:immunology; D008297:Male; D010084:Oxidation-Reduction; D011220:Pralidoxime Compounds; D011817:Rabbits; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis; D014228:Triaziquone / Q000494:pharmacology
D000477:Alkylating Agents; D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D007074:Immunoglobulin G; D011220:Pralidoxime Compounds; D013438:Sulfhydryl Compounds; D014228:Triaziquone; C028797:pralidoxime