[Isolation and characterization of carbohydrate specific antibodies from anti-Candida utilis serum].
Mannan of Candida utilis was hydrolyzed in oligosaccharides of different chain lengths. Mannohexaose and mannopentaose were shown to be the immunodominant group of this mannan. The oligosaccharides obtained were bound to sepharose by way of beta-(p-aminophenyl) ethylamine. Immunoglobulins against mannohexaose down to mannodiose were obtained using affinity chromatography of the anti-C utilis serum. The affinity columns were filled with sepharose-oligosaccharide gels. The serological activity was proved by agglutinaion and precipitation.
D000908:Antibodies, Fungal / Q000032:analysis* / Q000302:isolation & purification; D002175:Candida / Q000032:analysis / Q000276:immunology*; D002846:Chromatography, Affinity; D008351:Mannans / Q000276:immunology*; D009844:Oligosaccharides / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology*
D000908:Antibodies, Fungal; D008351:Mannans; D009844:Oligosaccharides; D011134:Polysaccharides
Gewinnung und Charakterisierung kohlenhydrat- spezifischer antikörper aus anti-Candida utilis Serum.