Development and application of a radioimmunoassay for tetracycline.
Antiserum against tetracycline was produced in rabbits immunized with a tetracycline hapten conjugated to bovine serum albumin. The antiserum was used to develop a radioimmunoassay for tetracycline. As little was 1 ng of tetracycline can be detected in one sample. The antitetracycline antiserum showed a considerable degree of specificity since it cross-reacted only with chlortetracycline (70%). However, its cross-reactivity with oxytetracycline, doxycycline, demeclocycline, methacycline and rolitetracycline was only negligible. The radioimmunoassay of tetracycline was used to measure the systemic bioavailability of orally administered tetracycline (25 mg/kg) to a group of mongrel dogs. Detectable levels of tetracycline were exhibited within 30 min and maximum plasma concentrations of the drug occurred within 3 to 4 hr after dose administration.
D000818:Animals; D000918:Antibody Specificity; D004285:Dogs; D008722:Methods; D011863:Radioimmunoassay; D013754:Tetracyclines / Q000032:analysis* / Q000097:blood
D013754:Tetracyclines