title: [Performance and limitations of the analysis of HDL-cholesterol (author's transl)].
abstract: Comparing published data, three methods for HDL-cholesterol determination (phosphotungstate/MgCl2, dextran sulphate 500/MgCl2 and heparin/MnCl2) are reviewed with respect to accuracy, precision, sensitivity and liability to interference. All three methods are specific (complete precipitation of apolipoprotein B-containing lipoprotein) and sufficiently sensitive (only significant coprecipitation of HDL). A disadvantage of the heparin/MnCl2 method is incomplete precipitation of apolipoprotein B-containing lipoprotein in hypertriglyceridemic sera. Limitations of the diagnostic value of HDL-cholesterol analysis are discussed.
mesh_terms: D001053:Apolipoproteins / Q000097:blood; D055598:Chemical Phenomena; D002621:Chemistry; D002784:Cholesterol / Q000097:blood*; D008076:Cholesterol, HDL; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008345:Manganese; D008722:Methods; D010772:Phosphotungstic Acid
chemical_list: D001053:Apolipoproteins; D008076:Cholesterol, HDL; D008075:Lipoproteins, HDL; D010772:Phosphotungstic Acid; D008345:Manganese; D002784:Cholesterol
vernacular_title: Möglichkeiten und Grenzen der Analytik des HDL-Cholesterins.