[Factors causing oligomerization of wall-eyed pollock rhodopsin before and after solubilization by sodium dodecyl sulfate].
Using SDS-electrophoresis, it was shown that the addition of sodium ions to the solubilization medium causes oligomerization of wall-eyed pollock rhodopsin. Oligomerization is also caused by solubilization of the wall-eyed pollock rod outer segments by sodium cholate (deoxycholate), by changes in SDS concentration as well as by an increase of protein content in the solubilization mixture, freezing of solubilizate and treatment of the rod outer segments by sodium borohydrate upon illumination. Possible causes of wall-eyed pollock rhodopsin aggregation are discussed.
D000818:Animals; D005399:Fishes; D046911:Macromolecular Substances; D010786:Photoreceptor Cells / Q000378:metabolism; D012168:Retinal Pigments / Q000378:metabolism; D012243:Rhodopsin / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate; D012995:Solubility
D046911:Macromolecular Substances; D012168:Retinal Pigments; D012967:Sodium Dodecyl Sulfate; D012243:Rhodopsin
Faktory, vyzyvaiushchie oligomerizatsiiu rodopsina mintaia do i posle soliubilizatsii DS-Na.