A cryopreservative procedure for storing cultivated and uncultivated amniotic fluid cells in liquid nitrogen.
The cryopreservation of cultured and uncultured amniotic fluid cells is of special importance in prenatal diagnosis. The freezing procedure presented gave a cell recovery of about 95% for cultivated human amniotic fluid cells combined with a rapid appearance of mitosis after inoculation.
D000653:Amniotic Fluid / Q000166:cytology*; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D005615:Freezing; D006801:Humans; D009584:Nitrogen; D011247:Pregnancy; D011309:Preservation, Biological / Q000379:methods*
D009584:Nitrogen