Monoclonal anti-A from a hybrid-myeloma: evaluating as a blood grouping reagent.
A monoclonal anti-A antibody has been evaluated and found suitable for use as a potent routine ABO grouping reagent, without the use of additives. The IgM anti-A (MH2/6D4) is secreted into the tissue culture supernatant by a permanent line of cloned cells derived by fusion of anti-A producing spleen cells and a mouse myeloma cell line. This is a cost-effective reagent which should reduce production costs by over 50%. This reagent has the advantages inherent in monoclonal antibodies among them the availability of unlimited quantities of unvarying antibody of known properties.
D000017:ABO Blood-Group System / Q000276:immunology*; D000818:Animals; D000906:Antibodies; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D002460:Cell Line; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D009101:Multiple Myeloma; D013154:Spleen / Q000166:cytology
D000017:ABO Blood-Group System; D000906:Antibodies; D007075:Immunoglobulin M