Investigation of cell patterning in the asexual fruiting body of Dictyostelium discoideum using haploid and isogenic diploid strains.
The cell patterning (proportion of spores, stalk cells, and basal disk cells) of individual asexual fruiting bodies of haploid and isogenic diploid strains of D. discoideum was examined to test the hypothesis that the patterning mechanism is based on the 'sensing' of only a single parameter, e.g., cell volume, in dividing the aggregate into the three cell types. If cell patterning is based on sensing only a single parameter, there is no reason to predict a change in cell patterning with ploidy change, and thus haploid and isogenic diploid strains should not differ in their cell patterning. The cell patterning of each of the three pairs of haploid and isogenic diploid strains examined was different. Therefore we conclude that the cell patterning mechanism must involve at least two components not changing in the same way with change in ploidy. The cell patterning of both the haploid and the diploid strains was qualitatively similar, i.e., relationships between the three cell types were described by equations of the same form in the haploid and diploid strains. However a quantitative change in cell patterning led to an increased percentage of stalk and basal disk cells in each diploid compared to its parent haploid. The ratio of basal disk to stalk cell was also greater in the diploids than in their parent haploids. We conclude that these are general ploidy-related changes because the cell patterning of each of the three parent haploid strains was different; the average percentage of stalk cells was 11.6% for X22 (12.4% for its diploid DU162), 20.5% for NP73 (27.2% for its diploid DP62), and 24.5% for HU127 (29.1% for its diploid DU310). One possible patterning mechanism could involve a diffusible signal (s), which shows gene dosage, interacting with cell-surface molecules which we predict occupy a limited number of sites per unit area of the cell membrane. The observed change in cell patterning leading to an increased percentage of stalk cells in diploid strains is predicted from such a model involving diffusible signal interacting with cell-surface molecules.
D002449:Cell Aggregation; D002454:Cell Differentiation; D004023:Dictyostelium / Q000166:cytology / Q000235:genetics*; D004171:Diploidy; D006238:Haploidy; D013172:Spores, Fungal