title: Competitive binding studies of compounds that interact with DNA utilizing fluorescence polarization.
abstract: The increase in the fluorescence polarization of acridine orange upon binding to DNA molecules is used as the basis of a competitive method to study the interaction of a variety of fluorescent and non-fluorescent compounds with DNA. Test compounds that interact with DNA inhibit both the binding of acridine orange to DNA and the accompanying increase in fluorescence polarization. Actinomycin D exhibits a dose-dependent inhibition of acridine orange-DNA binding with Micrococcus luteus DNA, calf thymus DNA, and poly(dG-dC); no detectable inhibition of acridine orange intercalation into poly(dA-dT) is observed. In contrast, proflavine shows similar acridine orange inhibition for poly(dA-dT), calf thymus DNA and M. luteus DNA.
mesh_terms: D000165:Acridine Orange / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D005454:Fluorescence Polarization; D008837:Micrococcus; D011067:Poly dA-dT / Q000378:metabolism; D011089:Polydeoxyribonucleotides / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D011089:Polydeoxyribonucleotides; D003609:Dactinomycin; D011067:Poly dA-dT; C019530:poly(dC-dG); D004247:DNA; D000165:Acridine Orange