Effect of 3-methylcholanthrene on RNA polymerase and protein kinase activities and on the nuclear ultrastructure of rat liver.
The polycyclic aromatic hydrocarbon 3-methylcholanthrene (3-MC) is a well known inducer of the microsomal mixed function oxidase enzyme system in rat liver cells. It seems probable that the inductive action of 3-MC is realized, at least partly, at the transcriptional level of protein synthesis regulation. The present experiments indicated that in the liver of young rats there was a significant alteration in the activities of nucleolar as well as nucleoplasmic protein kinase and RNA polymerase enzymes during the first days of exposure to a single dose of 3-MC. The ultrastructural investigations showed that 3-MC treatment caused nucleolar hypertrophy and accumulation of lipid inclusions in the nucleoplasm.
D000818:Animals; D002466:Cell Nucleolus / Q000201:enzymology / Q000648:ultrastructure; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D008748:Methylcholanthrene / Q000494:pharmacology*; D008854:Microscopy, Electron; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats
D008748:Methylcholanthrene; D011494:Protein Kinases; D012321:DNA-Directed RNA Polymerases