title: Dibutylchloromethyltin chloride, a potent inhibitor of electron transport in plant mitochondria.
abstract: Dibutylchloromethyl tin chloride (DBCT) inhibits coupled and uncoupled respiration of mitochondria from potato tubers, cauliflower florets and etiolated mung bean hypocotyls with succinate and L-malate but not with external NADH or TMPD/ascorbate as substrates. Using potato and cauliflower mitochondria. DBCT at 200 pmole/mg of protein gives complete inhibition  only in KCl-based media and at pH 6.8. DBCT has no effect on the internal pH of mung bean mitochondria, but does cause a decrease in the membrane potential. Electron transport through the alternative oxidase is not inhibited, neither is the ATP-synthase system. DBCT appears to interact with the functionally-distinct pool of ubiquinone associated with the oxidation of succinate and L-malate.
mesh_terms: D004579:Electron Transport / Q000187:drug effects*; D008928:Mitochondria / Q000187:drug effects*; D009947:Organotin Compounds / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects; D010944:Plants / Q000187:drug effects* / Q000378:metabolism; D014220:Trialkyltin Compounds
chemical_list: D009947:Organotin Compounds; D014220:Trialkyltin Compounds; C015129:dibutylchloro(chloromethyl)tin