Blood preservation 35. Red cell 2,3-DPG and ATP maintained by DHA-ascorbate-phosphate.
DHA (dihydroxyacetone, 60 mM) with ascorbic acid (d-ascorbate, 10 mM) kept 2,3-DPG concentrations above normal for six weeks. Levels of 2,3-DPG were below normal after four weeks with DHA alone and after two weeks with DHA-ascorbate-phosphate. As in previous studies, high phosphate concentrations decreased 2,3-DPG maintenance. ATP maintenance was best achieved with the following (in order of performance): DHA-phosphate (20 mM); DHA-phosphate (10 mM); the control, CPD-adenine preservative; Phosphate 20 mM; and DHA. DHA with ascorbate provides normal 2,3-DPG for six weeks. The adverse effects of DHA and DHA with ascorbate on ATP levels are modified by 10 mM phosphate.
D000225:Adenine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000097:blood*; D001205:Ascorbic Acid / Q000494:pharmacology; D001793:Blood Preservation; D002951:Citrates / Q000494:pharmacology; D004098:Dihydroxyacetone / Q000494:pharmacology; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010710:Phosphates / Q000494:pharmacology; D013997:Time Factors
D002951:Citrates; D004163:Diphosphoglyceric Acids; D010710:Phosphates; D000255:Adenosine Triphosphate; D000225:Adenine; D004098:Dihydroxyacetone; D001205:Ascorbic Acid