Neutral amino acid transport during the cell cycle of cultured human lymphocytes.
Neutral amino acid (isoleucine, leucine, and valine) transport was monitored in synchronous populations of WIL-2 cells, a diploid human lymphoblastoid B cell line. Cells were synchronized by either the double thymidine block technique or by density-dependent arrest in G1. Cells synchronized by these methods showed up to a 66% decrease in amino acid transport upon progressing from the early S phase through the G2 and M phases with a concomitant increase in transport during the G1 phase. The lowest activities of amino acid transport in synchronized cells were observed during the G2 and M phases.
D000597:Amino Acids, Branched-Chain / Q000378:metabolism*; D001692:Biological Transport; D002453:Cell Cycle; D002473:Cell Wall; D002478:Cells, Cultured; D006801:Humans; D007532:Isoleucine / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism*; D014633:Valine / Q000378:metabolism
D000597:Amino Acids, Branched-Chain; D007532:Isoleucine; D007930:Leucine; D014633:Valine