Separation of enantiomeric amines by ion-pair chromatography.
A high-performance liquid chromatographic method for the separation of optical isomers (enantiomers) of amines is described. It is based on ion-pair chromatography with a chiral counter ion in a system with an organic mobile phase and an adsorbing stationary phase. The method has been applied to enantiomers of 1-aryloxy-3-isopropylamine-2-propanol derivatives (alprenolol, metoprolol, propranolol) which are completely resolved with (+)-10-camphorsulphonate as the counter ion. Studies of the influence of the counter-ion structure and the mobile phase composition are presented.
D000526:Alprenolol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008790:Metoprolol / Q000032:analysis*; D011412:Propanolamines / Q000032:analysis*; D011433:Propranolol / Q000032:analysis*
D011412:Propanolamines; D000526:Alprenolol; D011433:Propranolol; D008790:Metoprolol