[Hemoglobins, XXXVI: The primary structure of a dimeric insect hemoglobin (Erythrocruorin), component IX from Chironomus thummi thummi. Studies on the quarternary structure of the dimeric CTT-hemoglobins (author's transl)].
The primary structure of a dimeric insect hemoglobin (erythrocruorin), component CTT IX (Chironomus thummi thummi) was established by automatic sequence analysis. The alignment of the peptides was facilitated by producing only a few large fragments. The primary structure of CTT IX is compared with the human beta-chains and with CTT III. It is discussed, why for the dimeric CTT-hemoglobins only dimerisation can be observed but no tetramerisation. Experiments were done to locate the binding areas between the subunits in the dimeric molecule. Even after blocking of the alpha-NH2-group by cyanate, the stability of the dimeric molecule is not altered. Therefore the binding regions of the CTT-hemoglobins must be different from those of the tetrameric hemoglobins of vertebrates. Our results lead to a quarternary structure different from that of the hemoglobins of mammalians. This structure explains the possibility of dimerisation, but excludes tetramerisation.
D000595:Amino Acid Sequence; D002683:Chironomidae / Q000032:analysis*; D003488:Cyanogen Bromide; D004175:Diptera / Q000032:analysis*; D004902:Erythrocruorins; D006454:Hemoglobins; D046911:Macromolecular Substances; D010446:Peptide Fragments / Q000032:analysis; D011487:Protein Conformation
D004902:Erythrocruorins; D006454:Hemoglobins; D046911:Macromolecular Substances; D010446:Peptide Fragments; D003488:Cyanogen Bromide
Hämoglobine, XXXVI[1,2] Die Primärstruktur eines dimeren Insektenhämoglobins (ERythrocruorin) (CTT IX) (Chironomus thummi thummi. Versuche zur Quartärstruktur der dimeren CTT-Hämoglobine.