title: Thermodynamic nonideality and the dependence of partition coefficient upon solute concentration in exclusion chromatography. Application to self-associating and non-self-associating solutes. Application to hemoglobin.
abstract: The theory of partition of macromolecular solute between mobile and stationary phases under highly nonideal conditions is reexamined. Expressions are derived for the dependence of the partition coefficient of a non-self-associating solute, and the weight average partition coefficient of a dimerizing solute, upon the total concentration of solute in the mobile phase. These expressions are based upon a treatment of nonideality in the stationary phase which is more realistic than that proposed earlier [L.W. Nichol, R.J. Siezin and D.J. Winzor, Biophys. Chem. 9 (1979) 17]. It is found that the chromatographic data on oxyhemoglobin in the above paper may be accommodated by the present theory without invoking self-association.
mesh_terms: D002850:Chromatography, Gel; D006454:Hemoglobins / Q000302:isolation & purification*; D046911:Macromolecular Substances; D008433:Mathematics; D011487:Protein Conformation; D012996:Solutions; D013816:Thermodynamics
chemical_list: D006454:Hemoglobins; D046911:Macromolecular Substances; D012996:Solutions