Sulfur amino acid metabolism in the developing rhesus monkey brain: subcellular studies of the methylation cycle and cystathionine beta-synthase.
The subcellular distributions of the enzymes associated with the methylation and cystathionine-synthesizing portion of the sulfur amino acid metabolic pathway have been determined in the occipital lobe of the rhesus monkey. 5-Methyltetrahydrofolate-homocysteine methyltransferase and 5, 10-methylenetetrahydrofolate reductase activities are located mainly in the soluble compartment. Serine hydroxymethyltransferase activity is located primarily in mitochondria. Cystathionine beta-synthase is a soluble enzyme with a significant component occluded within the nerve endings. Glycine, serine, and cystathionine increase per gram of tissue during development. Glycine and serine are approximately 30% occluded within the nerve endings. These data are consistent with a localization of sulfur amino acid metabolism that supports a differential compartmentation of potential neurotransmitter function and methylation function in the primate.
D000603:Amino Acids, Sulfur / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D003541:Cystathionine beta-Synthase / Q000378:metabolism*; D005260:Female; D006836:Hydro-Lyases / Q000378:metabolism*; D008251:Macaca / Q000378:metabolism*; D008253:Macaca mulatta / Q000378:metabolism*; D008745:Methylation; D011247:Pregnancy; D013347:Subcellular Fractions / Q000201:enzymology
D000603:Amino Acids, Sulfur; D006836:Hydro-Lyases; D003541:Cystathionine beta-Synthase