Enzymatic determination of lipoprotein-X, a specific serum cholestasis marker.
Non-esterified cholesterol was determined enzymatically with the cholesterol oxidase-peroxidase reaction using phenol and 4-aminophenazone as chromogens. This assay as done in the supernatant containing the lipoprotein-X. Other serum lipoproteins were removed by precipitation with heparin and zinc acetate followed by centrifugation. No standardization was required, the lipoprotein-X concentration being calculated with the use of the constant.
D002779:Cholestasis / Q000097:blood*; D002784:Cholesterol / Q000097:blood; D002789:Cholesterol Oxidase; D005260:Female; D006735:Horseradish Peroxidase; D006801:Humans; D008073:Lipoprotein-X / Q000097:blood*; D008297:Male
D008073:Lipoprotein-X; D002784:Cholesterol; D002789:Cholesterol Oxidase; D006735:Horseradish Peroxidase