High-performance liquid chromatographic procedure for the simultaneous determination of the natural polyamines and their monoacetyl derivatives.
The separation of the natural polyamines and their monoacetyl derivatives by high-performance reversed-phase liquid chromatography is reported. Octane sulfonate was used to form ion pairs with the polycations and acetylspermidine determinations directly from tissue extracts and body fluids without pre-purification.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008099:Liver / Q000032:analysis; D008297:Male; D009795:Octanes; D011073:Polyamines / Q000032:analysis* / Q000652:urine; D051381:Rats; D013095:Spermidine / Q000031:analogs & derivatives* / Q000032:analysis; D009764:o-Phthalaldehyde
D009795:Octanes; D011073:Polyamines; C017988:N(1)-acetylspermidine; D009764:o-Phthalaldehyde; D013095:Spermidine; C026728:octane