[some features of nuclear genome transcription at early stages of liver regeneration. RNA-polymerase activity of isolated nuclei].
The RNA-polymerase activity of isolated nuclei after 1, 3, 6, 12 and 24 hrs following partial hepatectomy was studied. The enzyme activity showed almost a 2-fold decrease after the first post-operative hour, followed by an increase of activity which reached its maximum after 6 hrs. A decrease of nuclear hepatocyte RNA-polymerase sensitivity to alpha-amanitine after 1 and 3 hrs following the surgery was found. After 6 hrs the enzyme was activated by alpha-amanitine. Using selective incorporation of four labelled ribonucleoside triphosphates, it was shown that alpha-amanitine causes a shift in the nucleotide composition of RNA synthesized in the regenerating liver towards the AU-type RNA.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005796:Genes; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008115:Liver Regeneration / Q000187:drug effects; D008297:Male; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects
D000546:Amanitins; D012321:DNA-Directed RNA Polymerases
Osobennosti transkriptsii iadernogo genoma na rannikh etapakh regeneratsii pecheni. RNK-polimeraznaia aktivnost' izolirovannykh iader.