Vascular changes in rabbit skin induced by proinflammatory phorbol and 12-deoxyphorbol esters.
The effects of proinflammatory phorbol and 12-deoxyphorbol esters were determined on blood flow changes and plasma exudation in rabbit skin. Blood flow changes were measured by means of 133Xe washout over that in control-treated sites, while plasma exudation was measured by 131I-labeled human serum albumin leakage from skin blood vessels. 12-Deoxyphorbol-13-phenylacetate, 12-deoxyphorbol-13-angelate, and their C-20 acetates induced vasoconstriction in rabbit skin in doses of 100 ng/site. Tetradecanoyl phorbol acetate indiced vasoconstriction to a far lower degree, while the parent alcohol phorbol and 9,13,14-orthophenylacetyl-resiniferonol had no significant effects on rabbit microvasculature. The ability of tigliane esters to contract rabbit blood vessels was confirmed in vitro in that each of these esters induced a prolonged contraction of superfused rabbit aorta in doses of 1-5 microgram. Plasma exudation in rabbit skin was not significantly increased by the phorbol and 12-deoxyphorbol esters, but in a second determination using 100 ng of PGE1 together with 100 ng of ester, plasma exudations of up to 40 microliter per site were recorded.
D000818:Animals; D001011:Aorta / Q000187:drug effects; D005122:Exudates and Transudates / Q000187:drug effects*; D007249:Inflammation / Q000209:etiology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D010703:Phorbol Esters / Q000494:pharmacology*; D010704:Phorbols / Q000494:pharmacology*; D011458:Prostaglandins E / Q000494:pharmacology; D011817:Rabbits; D012867:Skin / Q000098:blood supply*; D014661:Vasoconstriction / Q000187:drug effects
D010703:Phorbol Esters; D010704:Phorbols; D011458:Prostaglandins E; C029705:12-deoxyphorbolphenylacetate-20-acetate; C029780:12-deoxyphorbol-13-angelate