Effect of phosphoenolpyruvate analogs on the activity of enoylpyruvate transferase and the effect of UDP-N-acetylglucosamine on the reactivity of the active site SH group.
Effect of several phosphoenolpyruvate analogs on the activity of enoylpyruvate (phosphoenolpyruvate: UDP-2-acetamido-2-deoxy-D-glucose 2-enoyl-1-carboxyethyltransferase, EC 2.5.1.7) transferase was examined. The results suggest that the phosphoenolpyruvate binding site of the transferase is very similar to that of pyruvate kinase (ATP: pyruvate 2-O-phosphotransferase, EC 2.7.1.40). Evidence is presented to show that the binding of UDP-GlcNAc to the transferase enhances the reactivity of the active site SH group.
D019883:Alkyl and Aryl Transferases; D001665:Binding Sites; D066298:In Vitro Techniques; D007461:Iodoacetates / Q000494:pharmacology; D010728:Phosphoenolpyruvate / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology; D011770:Pyruvate Kinase / Q000378:metabolism; D013438:Sulfhydryl Compounds; D014166:Transferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D014539:Uridine Diphosphate Sugars / Q000494:pharmacology*
D007461:Iodoacetates; D013438:Sulfhydryl Compounds; D014539:Uridine Diphosphate Sugars; D014537:Uridine Diphosphate N-Acetylglucosamine; D010728:Phosphoenolpyruvate; D014166:Transferases; D019883:Alkyl and Aryl Transferases; C022566:UDP-N-acetylglucosamine 1-carboxyvinyltransferase; D011770:Pyruvate Kinase