A method for rapid filling of fine-tipped micropipettes with electrolyte solutions including those containing horseradish peroxidase.
Cooling the tips of micropipettes pulled from filament-containing capillary glass dramatically increases the rate and completeness of filling with a variety of electrolyte solutions. In tests with solutions containing horseradish peroxidase this method increased the completeness of filling by more than two orders of magnitude.
D000818:Animals; D004864:Equipment and Supplies; D006735:Horseradish Peroxidase; D008832:Microchemistry; D009424:Nervous System Physiological Phenomena
D006735:Horseradish Peroxidase