title: Use of a dried blood spot in immunoreactive-trypsin assay for detection of cystic fibrosis in infants.
abstract: We assayed more than 5000 blood spots dried on filter paper and approximately 1000 serum samples for immunoreactive trypsin, with commercial reagents (Behring and Sorin). The assay procedures were modified so that newborn screening is technically feasible. Both kits are satisfactory for serum assay, but the Sorin materials are better adapted for blood spot analysis. Immunoreactive trypsin in blood spots rapidly decreases with specimen age, but is stable in frozen serum. Values for premature infants do not differ significantly from those for full-term babies. Children with cystic fibrosis were readily distinguished from those without, up to at least one year of age.
mesh_terms: D001800:Blood Specimen Collection; D004796:Clinical Enzyme Tests; D003550:Cystic Fibrosis / Q000175:diagnosis*; D005615:Freezing; D006801:Humans; D007118:Immunoassay; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000175:diagnosis*; D007234:Infant, Premature; D008403:Mass Screening; D011933:Reagent Kits, Diagnostic; D012016:Reference Values; D014357:Trypsin / Q000097:blood*
chemical_list: D011933:Reagent Kits, Diagnostic; D014357:Trypsin