Correlation between alanine inhibition of pyruvate and composition of K-M hybrids.
A method is described for determination of the percentage of K-subunits in the K-M set of pyruvate kinase. The method is based on the alanine inhibition of the K-subunits. A linear correlation (r = 0.99) is found between the alanine inhibition and the percentage of K-subunits as calculated from the electropherograms, assuming (a) a subunit distribution as indicated by the suffix in K4, K3M, K2M2, etc., and (b) an equal contribution of K- and M-subunits to the intensity of the stain.
D000409:Alanine / Q000494:pharmacology*; D001921:Brain / Q000201:enzymology*; D001932:Brain Neoplasms / Q000201:enzymology*; D005910:Glioma / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000037:antagonists & inhibitors; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D046911:Macromolecular Substances; D009132:Muscles / Q000201:enzymology; D055503:Protein Multimerization; D011770:Pyruvate Kinase / Q000037:antagonists & inhibitors
D007527:Isoenzymes; D046911:Macromolecular Substances; D011770:Pyruvate Kinase; D000409:Alanine