[Modification of the luciferrin-luciferase method of determining ATP concentration in erythrocytes].
Modification of luciferine-luciferase method of determining ATP concentration in erythrocytes is proposed which takes 1.5-2 min from the moment of sample selection to obtaining the result. The method is based on the phenomenon that inhibition of luciferase reaction by an increase of ionic strength (above 200 m-ekv) results in the reaction change. Luminescence intensity rapidly reaches some level slightly changing in time. Successive repeated additions of ATP increase this level in proportion which makes possible to introduce the calibrating ATP solution into the sample under measurement after the intensity has reached the constant value. The method allows measurings of ATP concentration in aqueous hemolysates of erythrocytes or integral blood without protein precipitation.
D000255:Adenosine Triphosphate / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis*; D049454:Firefly Luciferin; D006801:Humans; D008156:Luciferases; D008722:Methods
D049454:Firefly Luciferin; D000255:Adenosine Triphosphate; D008156:Luciferases
Modifikatsiia liutsiferin-liutsiferaznogi metoda opredeleniia kontsentratsii ATF v éritrotsitakh]