Improved procedure for the anion-exchange isolation of urinary organic acids.
DEAE-Sephadex equilibrated in 0.5 M triethylammonium acetate is suitable for the quantitative isolation of lactonisable organic acids. Mono-, di- and tricarboxylic acids can be eluted sequentially from DEAE-Sephadex by the use of 0.5 M triethylamine, 0.5 M triethylamine--0.1 M acetic acid, and 1.5 M pyridinium acetate.
D002264:Carboxylic Acids / Q000652:urine*; D002849:Chromatography, Gas / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003637:DEAE-Dextran / Q000031:analogs & derivatives; D003998:Dicarboxylic Acids / Q000652:urine; D006801:Humans; D007202:Indicators and Reagents; D014233:Tricarboxylic Acids / Q000652:urine
D002264:Carboxylic Acids; D003998:Dicarboxylic Acids; D007202:Indicators and Reagents; D014233:Tricarboxylic Acids; C030237:DEAE-sephadex A-25; D003637:DEAE-Dextran