Preparation of purified toxoplasma gondii suspensions.
Simple methods for the preparation of purified suspensions of T. gondii zoites from mouse peritoneal exudate and HeLa tissue cultures are described. The suspensions from the mouse peritoneal exudate were prepared by mechanical disintegration of host cells and tryptic digestion. The average yield of purified Toxoplasma was 78%, with average purity of 99.96%. The suspensions from the tissue cultures were prepared by differential centrifugation and tryptic digestion. The average yield of Toxoplasma was 67% with average purity of 99.67%. An advantage of these procedures is their feasibility.
D000818:Animals; D001202:Ascitic Fluid / Q000469:parasitology; D002498:Centrifugation; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D010274:Parasitology / Q000379:methods*; D014122:Toxoplasma / Q000302:isolation & purification*; D014357:Trypsin
D014357:Trypsin