title: Perfusate composition and edema formation in isolated rat lungs.
abstract: The effect of perfusate composition on duration of lung perfusion until development of alveolar edema was evaluated in isolated ventilated rat lungs perfused at a rate of 25 ml/min in a recirculating system. When the perfusate was Krebs-Ringer bicarbonate solution (KRB) alone, alveolar edema developed in 40-85 min. Addition of glucose (5 mM) slightly prolonged the time to edema while addition of 3% fatty acid-poor bovine serum albumin (BSA) extended mean survival to 3.5 hr. With KRB containing both glucose and BSA, mean survival was greater than 4 hr and three of eight lungs had not become edematous when the experiments were terminated at 5 hr. Similar results were obtained when a synthetic plasma-stimulating solution (SPSS) that is essentially free of protein or other colloid was used as a perfusate. Perfusion at reduced flow rates (12 ml/min) with KRB plus glucose and BSA or with SPSS gave 5-hr survival rates of 100%. These results indicate that prolonged lung perfusion is possible with a colloid-free artificial medium and suggest that both mechanical and metabolic factors are important in maintaining isolated perfused rat lungs free of alveolar edema.
mesh_terms: D000227:Adenine Nucleotides / Q000096:biosynthesis; D000818:Animals; D004487:Edema / Q000139:chemically induced*; D005947:Glucose / Q000494:pharmacology; D007773:Lactates / Q000096:biosynthesis; D008168:Lung / Q000503:physiopathology*; D008297:Male; D010477:Perfusion; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology
chemical_list: D000227:Adenine Nucleotides; D007773:Lactates; D012710:Serum Albumin, Bovine; D005947:Glucose