Quantitation of 6-mercaptopurine in biologic fluids using high-performance liquid chromatography: a selective and novel procedure.
A simple, selective, sensitive and rapid procedure is described for the quantitation of 6-mercaptopurine  (6-MP) in biological fluids. A sensitivity of at least 5 ng/ml is easily achieved in plasma on a reversed-phase octadecylsilane (C18) column using a high-performance liquid chromatography system following an initial protein precipitation and a clean-up step. Mean extractability of the drug from plasma following this procedure is greater than 98% and the overall coefficient of variation for the assay is below 6%. Plasma levels of 6-MP were measured in a rhesus monkey for 12 h following an intravenous administration of a single bolus dose (4 mg/kg) of 6-MP.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008253:Macaca mulatta; D015122:Mercaptopurine / Q000097:blood* / Q000494:pharmacology / Q000652:urine; D012016:Reference Values; D013997:Time Factors
D015122:Mercaptopurine