[Increase in the resistance of a liposome-incorporated rubomycin-DNA complex to the action of DNAses].
When DNA and the rubomycin-DNA complex were incorporated into liposomes containing stearylamine, about 90 per cent of their amounts associated with the liposomes, incubation of such liposomes for an hour at 25-50 degrees C being not accompanied by liberation of DNA and its complex. Only about 10 per cent of DNA associated with the liposomes was destroyed by DNase I. The liposomal DNA was more stable to ultrasound than free DNA. The molecular mass of DNA associated with the liposomes on treatment with ultrasound decreased 1.9 times, whereas that of free DNA decreased by more than 3 times. With an increase in the weight ratio of lipid/DNA the level of DNA degradation lowered. This is evidence of the fact that liposomes increase resistance of DNA and its complex to chemical and physical factors.
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA / Q000378:metabolism / Q000494:pharmacology*; D003630:Daunorubicin / Q000378:metabolism / Q000494:pharmacology*; D003851:Deoxyribonucleases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004338:Drug Combinations; D004789:Enzyme Activation; D008081:Liposomes / Q000378:metabolism / Q000494:pharmacology*; D008970:Molecular Weight; D013696:Temperature; D014465:Ultrasonics
D004338:Drug Combinations; D008081:Liposomes; D004247:DNA; D003851:Deoxyribonucleases; D003630:Daunorubicin
Povyshenie ustoĭchivosti zakliuchennogo v liposomy kompleksa rubomitsin-DNK k deistviiu DNK-az.