Lithium induces abnormal calcium-regulated PTH release in dispersed bovine parathyroid cells.
The effects of lithium on calcium-regulated parathyroid hormone (PTH) release were assessed in collagenase-dispersed bovine parathyroid cells. Preincubation for 4 hours with LiCl caused a dose-dependent increase in the concentration of calcium required for half-maximal inhibition of immunoreactive PTH release (the "set-point" for calcium). A significant increase in "set-point" was observed following exposure to 1.25 mM lithium, a concentration within the therapeutic range for man. This effect required preincubation with LiCl, was not seen with NaCl or KCl, and persisted despite removal of extracellular lithium. This lithium-induced abnormality in calcium-regulated PTH secretion may be a useful model system for analyzing the analogous defect in secretory control in primary hyperparathyroidism.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008094:Lithium / Q000494:pharmacology*; D010280:Parathyroid Glands / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000378:metabolism*
D010281:Parathyroid Hormone; D008094:Lithium; D002118:Calcium