Estimation of surface labelling on whole, critical point dried, cells by microprobe analysis in the scanning electron microscope.
A method is described whereby the relative amount of histochemical label, such as ferritin, provided it is exclusively attached to the surface of cells, can be estimated. The X-ray emission from the metal coating, deposited from a diode-sputter coating unit, is used to correct for the variations in the iron counts from the label which could occur due to changing analysis conditions.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis*; D004577:Electron Probe Microanalysis; D004910:Erythrocyte Membrane / Q000032:analysis; D005293:Ferritins / Q000032:analysis*; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D051379:Mice; D008855:Microscopy, Electron, Scanning
D005293:Ferritins