In vitro growth of some fastidious adenoviruses from stool specimens.
Sixty-seven stool specimens from 51 children, positive for adenoviruses by electron microscopy and negative for growth in human-embryo kidney cells, were tested for growth in Chang conjunctiva cells. Twenty-eight specimens caused a cytopathic effect over more than one passage in these cultures, and several adenovirus strains grew better at 33 degree C than at 37 degree C. Most of the culture-positive specimens also induced the development of adenovirus antigens in KB cells detectable by a group-specific indirect immunofluorescence test. Twenty-four of the 25 fastidious strains tested were antigenically related and were distinct from the established serotypes commonly isolated from stools.
D000260:Adenoviruses, Human / Q000254:growth & development* / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D002648:Child; D003228:Conjunctiva / Q000166:cytology; D003588:Cytopathogenic Effect, Viral; D005243:Feces / Q000382:microbiology*; D006801:Humans; D013696:Temperature
D000956:Antigens, Viral