A heterogeneous fluorescence immunoassay for gentamicin using a second antibody separation.
A heterogeneous fluorescence immunoassay (FIA) for gentamicin was developed using a second antibody separation. The separation of bound from free fluorescence label, removes a number of endogeneous fluorescent interferences. Correlation with a standard radioimmunoassay (RIA) for gentamicin was acceptable (r = 0.91). We conclude that a heterogeneous FIA is a precise, accurate, and convenient alternative to monitoring antibiotic levels.
D000818:Animals; D000906:Antibodies; D005839:Gentamicins / Q000276:immunology*; D006041:Goats; D006801:Humans; D007118:Immunoassay; D011817:Rabbits; D013050:Spectrometry, Fluorescence
D000906:Antibodies; D005839:Gentamicins