An improved technique for the freeze-fracture of cell cultures grown as monolayers.
An improved and rapid procedure for the freeze-fracture of cell cultures grown as monolayers is described. No additional equipment than normally used for freeze-fracturing is necessary and cell culture conditions are as usual on plastic dishes. Cells are grown on a plastic foil membrane so that they can be viewed with a light microscope. If necessary, while viewing with a binocular microscope, specimen holders are glued to the foil membrane over selected areas of the culture. The specimen holders with the attached cell culture are dissected out and processed for freeze-fracturing in a BIOETH 2005. Fracture planes occur along the cell surface as well as through entire cells. Analysis of corresponding fracture faces is possible.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D002478:Cells, Cultured / Q000648:ultrastructure*; D003593:Cytoplasm / Q000648:ultrastructure; D005614:Freeze Fracturing / Q000379:methods*; D006801:Humans; D008854:Microscopy, Electron; D009940:Organoids / Q000648:ultrastructure