In vitro studies in reproductive immunology. 3. Restoration of mitogenic activity of lymphocytes inhibited by seminal plasma in man.
Human seminal plasma, (HuSePl) is inhibitory to mitogen (PHA-M, Con-A, and PWM) lymphocyte activation. The inhibition does not require the continuous presence of HuSePl. Lymphocyte cultures remain inhibited after 24-hr contact with HuSePl and subsequent washings. Addition of fresh autochthonous lymphocytes or T cells but not B cells can restore the mitogenic activity of HuSePl-inhibited lymphocyte cultures. These observations suggest that T-lymphocyte in the male genital tract are surveilled by HuSePl and lack of inhibitory material could induce an immunologic response by spermatozoal antigens.
D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D007111:Immunity, Cellular; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008938:Mitosis / Q000187:drug effects*; D011043:Pokeweed Mitogens / Q000494:pharmacology; D012661:Semen / Q000502:physiology*
D011043:Pokeweed Mitogens; D003208:Concanavalin A