[Changes in the composition of nucleotide sequences in the transcriptionally active fraction of rat liver DNA during cortisol induction].
The renaturation kinetics of functionally different DNA fractions of rat liver isolated by modified phenol fractionation were studied. It was found that the transcriptionally active fraction of DNA (DNA-I), making up to approximately 20% of total DNA, is enriched with unique sequences. Under cortisol-induced DNA-dependent synthesis of RNA in the liver the amount of repeated sequences in this DNA fraction is increased (approximately by 10-11%), whereas the type of renaturation of a transcriptionally inactive major DNA fraction (DNA-II), making up to approximately 70% of total DNA, remains unchanged. The renaturation kinetics of a weakly extractable DNA fraction (DNA-III) making up to 5-10% of total DNA are similar to those of the major DNA fraction. Under cortisol-induced transcription the number of repeated sequences in this fraction is decreased as compared to DNA-III of intact liver (by approximately 10-12%). The increase of the number of repeated sequences observed after 4-8 hrs (i. e. peak of induction) is decreased after 16 hrs, resulting in a complete reconstitution of the original type of DNA-I renaturation on the 24th post-induction hour.
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009695:Nucleic Acid Renaturation; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects*
D004247:DNA; D006854:Hydrocortisone
Izmenenie sostava nukleotidnykh posledovatel'nosteĭ v transkriptsionno-aktivnoĭ fraktsii DNK pecheni krys pri induktsii kortizolom.