Transcriptional errors and ambiguity resulting from the presence of 1,N6-ethenoadenosine or 3,N4-ethenocytidine in polyribonucleotides.
1,N6-Ethenoadenosine (epsilon A) and 3,N4-Ethenocytidine (epsilon C) in copolymers with unmodified nucleosides were transcribed using DNA-dependent RNA polymerase in the presence of Mn2+. Nearest neighbor analysis of the products showed that epsilon A directed incorporation of A much greater than U greater than C while epsilon C directed the incorporation of U greater than or equal to A much greater than C Neither directed G into the complementary polymer. Such misincorporations resulting from epsilon A and epsilon C, compounds that are formed in vivo by the carcinogen vinyl chloride, may have a biological role as promutagens.
D000241:Adenosine / Q000031:analogs & derivatives* / Q000032:analysis; D001483:Base Sequence; D003562:Cytidine / Q000031:analogs & derivatives* / Q000032:analysis; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D007700:Kinetics; D008345:Manganese / Q000494:pharmacology; D011131:Polyribonucleotides; D013329:Structure-Activity Relationship; D013698:Templates, Genetic; D014158:Transcription, Genetic
C012962:3,N(4)-ethenocytidine; D011131:Polyribonucleotides; C007640:1,N(6)-ethenoadenosine; D008345:Manganese; D003562:Cytidine; D012321:DNA-Directed RNA Polymerases; D000241:Adenosine