Effects of antibiotics on protein synthesis and degradation in primary cultures of rat hepatocytes.
In primary hepatocyte cultures, maintained in a protein-free medium, streptomycin, penicillin, and Garamycin (gentamicin) all inhibited protein synthesis at concentrations above 0.1 mM. Some inhibition was also observed with the fungicide Mycostatin at 100 U/ml. Hepatocytic protein degradation was markedly inhibited by penicillin fat concentrations above 0.1 mM, whereas streptomycin and Garamycin only showed slight inhibition at concentrations in excess of 1 mM. None of the antibiotics had any detectable effect on the structural integrity (viability) of the cells.
D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005839:Gentamicins / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009761:Nystatin / Q000494:pharmacology; D010406:Penicillins / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D013307:Streptomycin / Q000494:pharmacology*
D005839:Gentamicins; D010406:Penicillins; D011506:Proteins; D009761:Nystatin; D013307:Streptomycin