Preparation of GTP sensitive adenylate cyclase from luteinized rat ovaries.
Previous studies have shown that human chorionic gonadotropin stimulation of rat ovarian adenylate cyclase activity is enhanced but not augmented by GTP. Extraction of crude rat luteal membranes with 2M urea induced a GTP augmentation of hCG stimulation of adenylate cyclase activity of 3.5 fold. This augmentation of hormonal stimulation of adenylate cyclase activity by GTP was not accompanied by a decrease in hormone binding such as has been observed with other hormonally stimulated adenylate cyclase.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D006063:Chorionic Gonadotropin / Q000502:physiology*; D002850:Chromatography, Gel; D003600:Cytosol / Q000201:enzymology; D005260:Female; D006160:Guanosine Triphosphate / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D010053:Ovary / Q000201:enzymology*; D051381:Rats; D014508:Urea
D006063:Chorionic Gonadotropin; D006160:Guanosine Triphosphate; D014508:Urea; D000262:Adenylyl Cyclases