title: Selection of plasma for hepatitis B immune globulin using a low cost radioimmunoassay.
abstract: Polystyrene beads coated with pasteurised polyethylene glycol-precipitated HBsAg were used to detect anti-HBs by a solid phase inhibition assay employing unreacted 125I-anti-HBs which had previously been "processed" by polyethylene glycol precipitation and used to test for the HBsAg. A weak anti-HBs (approximately 0.1 IU/ml) positive control was used to establish a target level of reactivity. 16,035 freshly donated units of blood, a 90-sample coded anti-HBs panel and a selection of hyperimmune globulins were investigated. All positive results were confirmed by a standard commercial assay. Antibody content of selected samples was quantitated in international units. A target level of anti-HBs activity is suggested for donor plasma to be fractionated for the production of hepatitis B immune globulin.
mesh_terms: D000327:Adsorption; D000914:Antibodies, Viral; D006509:Hepatitis B / Q000276:immunology; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D006801:Humans; D007136:Immunoglobulins; D010949:Plasma; D011863:Radioimmunoassay / Q000191:economics
chemical_list: D000914:Antibodies, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D007136:Immunoglobulins