The relationship between plasma membrane lipid composition and physical-chemical properties. I. Fluorescence polarization studies of fatty acid-altered EL4 tumor cell membranes.
EL4 cels were cultured with exogenous fatty acids under conditions that resulted in their incorporation into membranes phospholipids. The behavior of the fluorescent lipid probes diphenylhexatriene and perylene was monitored in intact EL4 cells and in isolated EL4 plasma membranes. In whole cells substituted with unsaturated fatty acids, there was always a marked decrease in the P value of both probes compared to the P value of the probes in unsubstituted cells. In whole cells substituted with saturated fatty acids, on the other hand, P values for both probes were unchanged compared to unsubstituted cells. In plasma membrane isolated from EL4 cells, no difference in P values for either probe was observed among membranes from unsubstituted, saturated fatty acid substituted or unsaturated fatty acid substituted cells, even when the degree of fatty acid substitution was quite substantial. Most of the fluorescent signal for both probes in whole cells appeared to come from cytoplasmic lipid droplets. The value of techniques such as fluorescent polarization from monitoring physical properties of membranes (such as 'fluidity') is discussed.
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure*; D004161:Diphenylhexatriene / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology*; D007942:Leukemia, Experimental / Q000648:ultrastructure*; D008563:Membrane Lipids / Q000502:physiology*; D051379:Mice; D008856:Microscopy, Fluorescence; D010569:Perylene / Q000494:pharmacology; D013329:Structure-Activity Relationship
D005227:Fatty Acids; D008563:Membrane Lipids; D004161:Diphenylhexatriene; D010569:Perylene