Fluoride ion as an NMR relaxation probe of galactose oxidase-substrate binding.
From the dependence on substrate concentration of fluoride ion spin-lattice and spin-spin paramagnetic relaxation rate enhancements, a value for the dissociation constant, Kd = 0.059 +/0 0.002 M, for the anaerobic binding of dihydroxyacetone (monomer) to the Cu(II) site of the enzyme galactose oxidase (D-galactose:oxygen 6-oxidoreductase, EC 1.1.3.9) has been obtained. This value for Kd lies between previously reported values for Km derived by use of classical Michaelis-Menten kinetics. An analogous calculation for the anaerobic binding of galactose to the enzyme yields Kd = 0.145 +/- 0.004 M, a value different from several reported Michaelis constants. F- NMR relaxation measurements on air-exposed samples of galactose and the enzyme yield a dissociation constant for the active site-oxidation product (presumed to be galactohexodialdose), Kd = 2.2 +/- 0.2 M, a value at least an order of magnitude larger than the Michaelis or dissociation constants calculated for the binding of galactose to the enzyme active site; no value for this constant had been reported previously. Some implications of the competition results for the type of substrate binding are discussed.
D000693:Anaerobiosis; D003300:Copper; D004098:Dihydroxyacetone; D005459:Fluorides; D005690:Galactose; D005692:Galactose Oxidase / Q000378:metabolism*; D007700:Kinetics; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008433:Mathematics; D011485:Protein Binding
D008024:Ligands; D003300:Copper; D005692:Galactose Oxidase; D004098:Dihydroxyacetone; D005459:Fluorides; D005690:Galactose