Repair of ultraviolet radiation damage in xeroderma pigmentosum cells belonging to complementation group F.
DNA-repair characteristics of xeroderma pigmentosum belonging to complementation group F were investigated. The cells exhibited an intermediate level of repair as measured in terms of (1) disappearance of T4 endonuclease-V-susceptible sites from DNA, (2) formation of ultraviolet-induced strand breaks in DNA, and (3) ultraviolet-induced unscheduled DNA synthesis during post-irradiation incubation. The impaired ability of XP3YO to perform unscheduled DNA synthesis was restored, to half the normal level, by the concomitant treatment with T4 endonuclease V and ultraviolet-inactivated Sendai virus. It is suggested that xeroderma pigmentosum cells of group F may be defective, at least in part, in the incision step of excision repair.
D000328:Adult; D002478:Cells, Cultured; D004247:DNA / Q000528:radiation effects*; D004260:DNA Repair; D004261:DNA Replication; D006801:Humans; D008297:Male; D010641:Phenotype; D012867:Skin / Q000166:cytology; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000235:genetics*
D004247:DNA