2-n-nonyl-4-hydroxy[3-3H]quinoline N-oxide: interaction with the mitochondrial membrane.
1. Synthesized 2-n-nonyl-4-hydroxy[3-3H]quinoline N-oxide ([3H]NoHOQnO) is a powerful inhibitor of both NADH oxidase and succinate oxidase activities in submitochondrial particles from beef heart. 2. The binding of [3H]NoHOQnO to submitochondrial particles reveals one specific binding site, which is sensitive to antimycin, and a large amount of aspecific binding. 3. The content of [3H]NoHOQnO-specific binding sites is related to the cytochrome b content in the particles. 4. The binding of [3H]NoHOQnO to the specific site causes the inhibition of electron transfer. 5. The relation between the inhibition of NADH oxidation and [3H]NoHOQnO-specific binding is hyperbolic.
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D001665:Binding Sites / Q000187:drug effects; D002417:Cattle; D003497:Cyclic N-Oxides / Q000378:metabolism / Q000494:pharmacology; D004579:Electron Transport / Q000187:drug effects; D006912:Hydroxyquinolines / Q000378:metabolism* / Q000494:pharmacology; D007425:Intracellular Membranes / Q000378:metabolism; D008929:Mitochondria, Heart / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors
D003497:Cyclic N-Oxides; D006912:Hydroxyquinolines; D009097:Multienzyme Complexes; C010494:nonyl-4-hydroxyquinoline-N-oxide; D000968:Antimycin A; D013385:Succinate Dehydrogenase; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases