Isolation and characteristics of hepatocytes.
The preparation of isolated hepatocytes for the investigation of liver metabolism in the intact cell is described, including some criteria to assess their metabolic competence. A comparison is made between parameters in the perfused liver and in hepatocytes. Freshly isolated hepatocytes are not readily suitable for metabolic studies.
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D002469:Cell Separation; D005958:Glucosephosphates / Q000378:metabolism; D006728:Hormones / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D010477:Perfusion / Q000295:instrumentation; D006005:Phosphorylases / Q000378:metabolism
D000227:Adenine Nucleotides; D005958:Glucosephosphates; D006728:Hormones; D006005:Phosphorylases