title: Analysis and preliminary purification of glycoproteins isolated from eggs in the urine of patients with Schistosoma haematobium infection.
abstract: Preliminary analysis and purification of glycoproteins from Schistosoma haematobium eggs were carried out with a small quantity of antigenic material obtained from the urine of infected human patients. A soluble egg extract was 125I-labeled and was fractionated by lectin affinity chromatography for separating egg glycoproteins. The crude glycoprotein fraction was run on SDS-PAGE to yield three polydisperse peaks with Rf values of 0.31, 0.57, and 0.84. 125I-labeled egg glycoproteins were further fractionated by ion exchange chromatography to yield four peaks or shoulders. One of these peaks constituted the major labeled egg glycoprotein of S. haematobium (MEGL-H) in a relatively pure form as determined by SDS-PAGE, and its estimated m.w. was 70,000. This glycoprotein was consistently and highly reactive serologically with a serum pool from schistosomiasis haematobia patients by a Farr-type radioimmunoassay (RIA). A limited cross-specificity study of inhibition RIA indicated that S. mansoni eggs contain components that cross-react only partially with MEGL-H. These results focus attention on MEGL-H as a potential serodiagnostic probe.
mesh_terms: D005260:Female; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D008970:Molecular Weight; D010063:Ovum / Q000032:analysis / Q000276:immunology; D011863:Radioimmunoassay; D012548:Schistosoma haematobium; D012552:Schistosomiasis / Q000652:urine*; D014556:Urine / Q000469:parasitology*
chemical_list: D006023:Glycoproteins