Selective removal of histone H1 from nucleosomes at low ionic strength.
The method for removal of histone H 1 from chromatin by treatment with ion-exchange resin AG 50 WX 2 in the presence of 100 mM NaCl and 50 mM phosphate buffer (Thoma and Koller, 1977, Cell, 12, 101-107) results in production not only of H1-depleted chromatin but also free DNA. We have not modified this procedure so that the nucleosome is treated with the cation exchange resin in two steps, first in 50 mM sodium phosphate buffer and then in 50 mM sodium phosphate and 50 mM NaCl whereby histone H 1 is selectively removed without a release of free DNA at low resin concentrations.
D002411:Cation Exchange Resins; D004247:DNA / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D006657:Histones / Q000302:isolation & purification*; D006358:Hot Temperature; D009707:Nucleosomes / Q000032:analysis*; D009994:Osmolar Concentration
D002411:Cation Exchange Resins; D006657:Histones; D009707:Nucleosomes; D004247:DNA