Expression of influenza A virus internal antigens on the surface of infected P815 cells.
A panel of hybridoma antibodies was used to study the expression of the 2 major internal influenza A virus proteins, matrix protein (M), and nucleoprotein (NP) on the surface of influenza A-infected P815 cells. Using a sensitive radioimmunoassay (RIA) performed with live infected P815 cells only minute amounts of M were detectable, whereas M was not detected in 3 other cell-surface assays. In contrast, NP was detected in 10-fold greater amounts than M and 10- to 50-fold lower amounts than the viral glycoproteins by RIA. Furthermore, the presence of NP could be demonstrated by immunofluorescence, microcytotoxicity, and agglutination assays performed on live infected cells.
D000327:Adsorption; D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D001666:Binding Sites, Antibody; D002460:Cell Line; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D006822:Hybrid Cells / Q000276:immunology; D009980:Influenza A virus / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D014764:Viral Proteins / Q000494:pharmacology
D000906:Antibodies; D000954:Antigens, Surface; D000956:Antigens, Viral; D014764:Viral Proteins