Alterations of the incorporation of [3H]thymidine in cells in culture regulated by nucleoplasmic extract.
Porcine skin nucleoplasmic extract (PSNE) was shown to alter the incorporation of [3H]thymidine into DNA of selected porcine, bovine, and human cell populations in culture. PSNE stimulated incorporation of [3H]thymidine into DNA of porcine and bovine dermal cells an average of 300 and 200% of control value, respectively. When porcine and bovine epidermal cells were exposed to PSNE the treatment inhibited [3H]thymidine incorporation into DNA by an average of 48 and 45%, respectively. Similar inhibitions were observed for porcine and bovine kidney, porcine lung, and human KB cells. Thus, the effect of PSNE on the incorporation of [3H]thymidine into DNA of various cultured cells was either stimulatory to dermal cells or inhibitory to a variety of other cell types, including skin epidermal cells. The stimulatory and inhibitory effects of PSNE were abolished by heating PSNE for 5 min in boiling water before its addition to cell cultures. This suggests that macromolecular structure is important in the action of PSNE.
D000818:Animals; D002417:Cattle; D002457:Cell Extracts / Q000494:pharmacology*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D012867:Skin / Q000378:metabolism*; D013045:Species Specificity; D013552:Swine / Q000378:metabolism*; D014020:Tissue Extracts / Q000494:pharmacology*
D002457:Cell Extracts; D014020:Tissue Extracts; D004247:DNA