Reversed-phase supports for th resolution of large denatured protein fragments.
Large pore (much greater than 300 A), spherical partical silica stationary phases possessing either C18 or C8 hydrocarbon ligands out-performed small pore (60-100A), irregular shaped silicas for the purification of large denatured peptides. Since columns 5 cm in length appeared to be as effective in separating peptides as columns 5 times longer, it is likely that large peptides absorb to the matrix rather than partition between the stationary and mobile phases.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003488:Cyanogen Bromide; D005260:Female; D005333:Fetus; D005914:Globins / Q000032:analysis*; D006801:Humans; D010446:Peptide Fragments / Q000302:isolation & purification*; D011247:Pregnancy
D010446:Peptide Fragments; D005914:Globins; D003488:Cyanogen Bromide