title: Separate isolation of cells from nodules and surrounding parenchyma of the same precancerous rat liver: biochemical and cytochemical characterization.
abstract: Various enzyme and metabolic alterations have been observed in the hyperplastic nodules which appear during the hepatocarcinogenesis. These alterations have been mainly specified by histochemical observations. In this report, a technique of hepatocyte isolation is described which enables the separation of 2 cellular fractions, respectively, from the nodules and from the surrounding parenchyma of the same liver of a rat previously treated with a hepatocarcinogen. Such a technique allowed parallel analysis of both cellular populations by biochemical and cytochemical techniques.
mesh_terms: D000818:Animals; D002469:Cell Separation; D006651:Histocytochemistry; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000648:ultrastructure; D008112:Liver Glycogen / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism*; D008297:Male; D011230:Precancerous Conditions / Q000378:metabolism*; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D008112:Liver Glycogen