Geometry of the phosphodiester backbone in the A form of deoxyribonucleic acid determined by phosphorus-31 nuclear magnetic resonance spectroscopy.
31P NMR spectra were obtained from poly(dA-dT) . poly(dA-dT) fibers which gave an X-ray diffraction pattern similar to that of the A form of natural deoxyribonucleic acid (DNA). The analysis of the line shape indicated that the A form of poly(dA-dT) . poly(dA-dT) has a single uniform backbone conformation; the orientation of the phosphodiester group relative to the helical axis was determined to be beta = 70 degrees and gamma = 52 degrees. The 31P NMR spectra of poly(dA-dT) . poly(dA-dT) were in remarkable contrast to the 31P NMR spectra of the A form of natural DNA, which exhibited an unusual line shape. The origins of the abnormalities in the line shape for the A form of natural DNA are discussed in terms of phosphodiester orientations.
D000818:Animals; D004247:DNA; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D010758:Phosphorus; D011067:Poly dA-dT; D011089:Polydeoxyribonucleotides; D012474:Salmon
D011089:Polydeoxyribonucleotides; D011067:Poly dA-dT; D010758:Phosphorus; D004247:DNA