Anaplasma marginale in bovine erythrocyte cultures.
Bovine erythrocytes infected with Anaplasma marginale were standardized with normal bovine erythrocytes at 1.4% and 3.5% parasitemia and cultured under low oxygen pressure at 37 C. The number of erythrocytes infected by A marginale appeared to increase 3.3-fold by day 11 and 4.5-fold by day 3, as determined by microscopic examination of Giemsa-stained thin films of the culture suspension. Anaplasma parasites in experimental cultures were proven to be viable and pathogenic by calf inoculation for up to 16 days.
D000709:Anaplasma / Q000254:growth & development* / Q000302:isolation & purification; D000712:Anaplasmosis / Q000382:microbiology; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D002478:Cells, Cultured; D004912:Erythrocytes / Q000382:microbiology*