title: Cellular localization of the multiple molecular forms of acetylcholinesterase in cultured neuronal cells.
abstract: The cellular localization of the molecular forms of acetylcholinesterase was explored in chick sympathetic neurons and in mouse T28 cells (neuroblastoma X sympathetic ganglion cell hybrids) using the reversible, poorly lipid-soluble inhibitor of acetylcholinesterase, BW284C51, to protect cell surface activity while inactivating cytoplasmic activity with DFP, an irreversible, lipid-soluble inhibitor. Our results show protection of over 80% of the chick 11 S form and over 90% of the corresponding mouse 10 S form under these conditions, while over 90% of the chick 6.5 S and mouse 4 S forms are inhibited. The results suggest that the avian 11 S and mouse 10 S forms are predominantly or exclusively ectoenzymes while the respective 6.5 S and 4 S forms are confined to the cytoplasm.
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis*; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002645:Chickens; D005728:Ganglia, Sympathetic / Q000201:enzymology; D006822:Hybrid Cells / Q000201:enzymology*; D007527:Isoenzymes / Q000032:analysis*; D051379:Mice; D008970:Molecular Weight; D009447:Neuroblastoma; D009474:Neurons / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; D000110:Acetylcholinesterase