title: Effect of adding cysteine to brain-heart infusion broth on the isolation of Bacteroides fragilis from experimental blood cultures.
abstract: Adding 0.05% cysteine to brain-heart infusion broth greatly improved the recovery of three strains of Bacteroides fragilis from simulated blood cultures. No bacteroides were isolated after 24 hours' incubation in brain-heart infusion broth without added cysteine, and this was therefore a poor medium for the isolation of B. fragilis. Results with brain-heart infusion broth containing 0.05% cysteine were similar to those obtained with commercially prepared Thiol and Brewer's thioglycollate media.
mesh_terms: D000818:Animals; D001441:Bacteroides fragilis / Q000302:isolation & purification*; D001769:Blood / Q000382:microbiology*; D001921:Brain; D003470:Culture Media; D003545:Cysteine; D006321:Heart
chemical_list: D003470:Culture Media; D003545:Cysteine