Agarose-linked xanthosine: a biospecific resin for guanine aminohydrolase.
Polymer-bound xanthosine (4) has been prepared. Condensation of xanthosine with ethyl 4-oxovalerate and saponification of the product gave 2',3'-O-[1-(2-carboxyethyl)ethylidene] xanthosine. The latter was coupled to 6-aminohexylagarose through its carboxylic group, to yield the polymer 4. The content of bound ligand was 8 mumol/g of moist gel, a value that agrees with the number of free amino groups determined by the trinitrobenzenesulfonic acid assay before coupling. Immobilised xanthosine was used as a biospecific resin (inhibitor resin) for guanine aminohydrolase (EC 3.5.4.3), to separate the enzyme from a mixture containing adenosine deaminase (EC 3.5.4.4).
D000243:Adenosine Deaminase; D000619:Aminohydrolases / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D006148:Guanine Deaminase / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008854:Microscopy, Electron; D011134:Polysaccharides; D012263:Ribonucleosides; D012685:Sepharose / Q000031:analogs & derivatives; D014970:Xanthines
D011134:Polysaccharides; D012263:Ribonucleosides; D014970:Xanthines; C024903:aminohexyl-sepharose; D012685:Sepharose; C005893:xanthosine; D000619:Aminohydrolases; D006148:Guanine Deaminase; D000243:Adenosine Deaminase