Liposome-encapsulated desferrioxamine in experimental iron overload.
We have encapsulated desferrioxamine (DF) in multilamellar liposomes (ML) and unilamellar liposomes (UL). Liposomes were prepared either with or without a glycolipid, i..e. galactocerebroside (GC). The average diameter of ML was 0.5 microgram, and that of UL was 0.08 microgram. Less tha 5% of DF leaked out from the liposomes after incubation in mouse plasma for 6 h. 59Fe-ferritin and 59Fe-labelled heat damaged erythrocytes (59Fe-DRBC) were administered to normal and hypertransfused mice as models of iron overload. Ferritin was used to label liver parenchymal cells and DRBC to label the Kupffer cells of the liver. A single injection of ML or UL with or without galactocerebroside into normal and hypertransfused mice enhanced from 3- to 15-fold the urinary excretion of radioiron from 59Fe-ferritin and from 59Fe-DRBC injected mice. For both the normal and hypertransfused mice, liposomes containing GC removed more 59Fe radioactivity from mice receiving 59Fe-DRBC. Thus GAC-liposomes may have a higher affinity for parenchymal cells of the liver, whereas liposomes without the glycolipid may have a higher uptake by the Kupffer cells.
D000818:Animals; D003676:Deferoxamine / Q000378:metabolism / Q000627:therapeutic use*; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005293:Ferritins / Q000378:metabolism; D006486:Hemosiderosis / Q000188:drug therapy*; D006358:Hot Temperature; D007501:Iron / Q000378:metabolism* / Q000652:urine; D008081:Liposomes / Q000008:administration & dosage; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D014018:Tissue Distribution
D008081:Liposomes; D005293:Ferritins; D007501:Iron; D003676:Deferoxamine