title: Catabolism of 3-O-methyl-D-glucopyranose by Cryptococcus laurentii.
abstract: When Cryptococcus laurentii is grown on 3-O-methyl-D-glucopyranose (3-O-MeGlc), two metabolic products are methanol and an extracellular mannan. Hydrolysis of the mannan produced during growth on 3-O-MeGlc labelled with 14C in the 1 or 6 position yields mannose with retention of the labelling pattern in the carbon chain. O-Methyl-labelled substrate yields radioactive methanol. Three possible mechanisms of O-demethylation are ether cleavage, peroxidatic attack, and beta-elimination. The latter mechanism was shown to be the most likely through the use of double-labelled (3H and 14C) 3-O-MeGlc.
mesh_terms: D019325:3-O-Methylglucose; D003454:Cryptococcus / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D010090:Oxidoreductases, O-Demethylating / Q000378:metabolism
chemical_list: D008757:Methylglucosides; D008759:Methylglycosides; D019325:3-O-Methylglucose; D010090:Oxidoreductases, O-Demethylating; D008358:Mannose