[Modified Gedelisa test (electrophoresis in gradient of polyacrylamide gel combined with Elisa test). Applications to Toxoplasma gondii exo-antigens (author's transl)].
The authors describe a modified Gedelisa test made up an electrophorese in gradient polyacrylamide gel plus an Elisa test. The gel gradient, after electrophoretic migration is cut longitudinaly. One half is stained with Coomassie blue and the second half is cut in slices of 1 mm thick. Each slice is placed in a well of a microtitration polystirene plate, filled up with carbonate buffer pH 9,6 and then scrached. Proteins diffuse out of the gel and coated on the polystirene. The antigenicity of the coated proteins is revealed by Elisa test. This technique was applied to the study of Toxoplasma gondii exo-antigens obtained from medium of in vitro culture on Vero cells. This antigen contains four fractions, three of them are stained with Coomassie blue, their molecular weights are 160 000, 830 000 and more than one million daltons.
D000941:Antigens / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D008970:Molecular Weight; D011506:Proteins; D014122:Toxoplasma / Q000276:immunology*
D000941:Antigens; D011506:Proteins
Le test Gedelisa (électrophorèse en gradient de gel de polyacrylamide combinée au test Elisa). Applications aux exo-antigènes de Toxoplasma gondii.