title: Concentration and purification of rubella virus hemagglutinin by hollow fiber ultrafiltration and sucrose density centrifugation.
abstract: Large volumes of rubella virus were produced in Vero cell monolayers which were grown in the Corbeil-Bellco TM system. Infectious tissue culture fluids were concentrated at least 600 times in less than 4h by ultrafiltration on hollow fibers with a molecular weight cutoff of 100 000. Recovery of the hemagglutinating activity was 75%. Rubella virus was purified by three successive sucrose density gradient centrifugations using a combination and discontinuous and linear gradients. Specific activity was increased 1000-fold.
mesh_terms: D002499:Centrifugation, Density Gradient; D006389:Hemagglutinins, Viral / Q000302:isolation & purification*; D012412:Rubella virus / Q000032:analysis*; D014462:Ultrafiltration; D014765:Viral Vaccines
chemical_list: D006389:Hemagglutinins, Viral; D014765:Viral Vaccines