Transformation of Streptococcus sanguis with monomeric pVA736 plasmid deoxyribonucleic acid.
Monomeric and oligomeric forms of a 5.0 x 10(6)-dalton plasmid (conferring erythromycin resistance) were able to genetically transform naturally competent Streptococcus sanguis. Transformation with electrophoretically purified monomer was a second-order process, whereas transformation with a dye-buoyant density gradient-purified plasmid preparation followed one-hit kinetics.
D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial; D004587:Electrophoresis, Agar Gel; D004917:Erythromycin / Q000494:pharmacology; D007700:Kinetics; D010957:Plasmids; D013298:Streptococcus sanguis / Q000235:genetics*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004917:Erythromycin