Isolation of plant tubulin from azuki bean epicotyls by ethyl N-phenylcarbamate-Sepharose affinity chromatography.
A new method for isolating plant tubulin was devised. The method involves affinity chromatography with ethyl N-phenylcarbamate and Sepharose. The protein isolated by the affinity chromatography and purified by two successive chromatographical steps on DEAE-Sephadex A-50 and Sephadex G-200 consisted of two subunits which possessed the same mobilities as the alpha- and beta-subunits of rabbit brain tubulin on SDS-polyacrylamide gel electrophoresis and showed colchicine-binding activity.
D000818:Animals; D001923:Brain Chemistry; D002846:Chromatography, Affinity; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D008970:Molecular Weight; D010944:Plants / Q000032:analysis*; D011817:Rabbits; D012685:Sepharose; D014404:Tubulin / Q000302:isolation & purification*
D007202:Indicators and Reagents; D046911:Macromolecular Substances; D014404:Tubulin; D012685:Sepharose