Hexose transport in Novikoff rat hepatoma cells. A simple carrier with directional symmetry, but variable relative mobilities of loaded and empty carrier.
The kinetics of transport of the non-metabolizable hexose, 3-O-methyl-D-glucose, have been measured in Novikoff rat hepatoma cells by both zero-trans entry and equilibrium exchange procedures. Transport conformed to a simple carrier model which operates symmetrically with respect to direction, but with greater mobility of the loaded than of the empty carrier. Although a complete kinetic description of the transporter can, in theory, be obtained by application of integrated equations describing the time course of substrate equilibrium across the membrane beginning from the zero-trans situation, statistical analysis of hypothetical data indicated that directional asymmetry or differential mobilities of loaded and empty carrier cannot be discerned reliably from such data alone. The difference in mobility of loaded and empty carrier, apparent in a comparison of zero-trans entry and exchange data, ranged from 1.5--7-fold in different batches of cells. It is concluded that the magnitude of the difference is not an inherent property of the transporter, but is determined physiologically, and may be involved in regulation of hexose transport.
D000818:Animals; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000378:metabolism*; D006601:Hexoses / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008433:Mathematics; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D051381:Rats
D002352:Carrier Proteins; D006601:Hexoses; D008757:Methylglucosides; D008759:Methylglycosides