Incorporation of saturated and cis- and trans-unsaturated long chain fatty acids in rat myocytes and increased susceptibility to arrhythmias.
Neonatal rat heart myocytes cultured for 3 days in fatty acid-enriched (5 X 10(5) M stearate, oleate, elaidate, linoleate, linoelaidate or arachidonate at a 3 : 1, fatty acid (FA/albumin ratio) were analyzed by high-pressure liquid chromatography (HPLC) for the FA content of their neutral lipid, glyco-cardiolipid and phospholipid fractions and were tested for their susceptibility to arrhythmias produced by hypoxic/normoxic medium changes. Increases in the relative amount of FA in the above lipid fractions were generally in the order: elaidate = linoelaidate greater than linoleate = arachidonate greater than stearate = oleate. Cell morphology was unaffected by such treatment, but the cultures were more susceptible to induced arrhythmias. Cultures enriched with trans-FA, elaidate or linoelaidate, became markedly arrhythmic; arrhythmias in stearate-enriched cultures were of short duration; whereas the other FA produced intermediate increases. Susceptibility to arrhythmias could not be correlated with polyunsaturated fatty acid (PUFA/saturated fatty acid (SATFA) ratios of FA in the phospholipid fraction.
D000818:Animals; D000831:Animals, Newborn; D001145:Arrhythmias, Cardiac / Q000139:chemically induced*; D002478:Cells, Cultured; D005227:Fatty Acids / Q000378:metabolism* / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000378:metabolism* / Q000494:pharmacology; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D051381:Rats
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids