title: A further examination of the active form of Crotalus adamanteus phospholipase A2.
abstract: The phospholipase A2 from Crotalus adamanteus venom has been shown to be active as the dimer or 30 000 molecular weight species, at concentrations used for enzyme assay (0.1--10 microgram/ml). Gel filtration of the enzyme in the presence of Ca2+ and monomeric concentrations of the substrate dihexanoylphosphatidylcholine showed that all the protein migrated as a 30 000 molecular weight species. Active enzyme sedimentation velocity experiments using the same conditions gave s020,W=2.85 +/- 0.05 S, which compares favorably with the value obtained at mg/ml concentrations (3.11 S). These results confirm the results of Shen et al. (Shen, B.W., Tsao, F.H.C, Law, J.H. and Kézdy, F.J. (1975) J. Am. Chem. Soc. 97, 1205--1208).
mesh_terms: D000818:Animals; D002498:Centrifugation; D002850:Chromatography, Gel; D003435:Crotalid Venoms; D008970:Molecular Weight; D010740:Phospholipases / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2
chemical_list: D003435:Crotalid Venoms; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2