[Use of the aliphatic amine reaction with 2,5-dinitrophenol in antibiotic analysis].
Possible use of dinitrophenol reaction with amines in quantitative determination of amines and amino sugars in various antibiotics was investigated. It was found that the reaction with 2,5-dinitrophenol (2,5-DNP) in dimethylsulfoxide (DMSO) providing the highest batochromic shift in the electron spectra was most suitable. Selectivity of the 2,5-DNP reaction with respect to mycosamine contained in polyenic antibiotics was shown. With the method of PMR it was demonstrated that DMSO formed a complex with 2,5-DMP. The electron spectral of the complexes of glucosamine, N-methylgycosamine and levorin with 2,5-DNP were used for calculation of the complex stability constants, which were practically the same. This provided the use of glucosamine as a standard for plotting the calibration curves. A quantitative method for determination of amines in polyenic antibiotics based on the above reaction was developed.
D000588:Amines / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000032:analysis*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology*; D011090:Polyenes / Q000032:analysis; D013054:Spectrophotometry, Atomic / Q000379:methods
D000588:Amines; D000900:Anti-Bacterial Agents; D004140:Dinitrophenols; D011090:Polyenes; C028909:2,5-dinitrophenol; D004121:Dimethyl Sulfoxide
Ispol'zovanie reaktsii alifaticheskikh aminov s 2,5-dinitrofenolom v analize antibiotikov.