title: Sex determination from plucked human hairs without epithelial root sheath. III. Fluorescent feulgen reaction using acriflavine.
abstract: In order to determine sex from plucked human hair roots devoid of epithelial root sheath, the frequency of the X-chromatin was examined in hair cortex nuclei of plucked hairs stained by the fluorescent Feulgen reaction using acriflavine. There was a distinct difference between male and female hairs, the frequency ranging from 27% to 70% (average 45.8%) in female samples and from 0% to 8% (average 2.9%) in male samples. The difference in the frequency between male and female was detected in samples kept in a dried condition for 32 weeks.
mesh_terms: D000166:Acridines; D000167:Acriflavine; D000293:Adolescent; D000328:Adult; D004396:Coloring Agents; D005260:Female; D005453:Fluorescence; D006197:Hair; D006801:Humans; D008297:Male; D008875:Middle Aged; D012394:Rosaniline Dyes; D012728:Sex Chromatin / Q000032:analysis; D012732:Sex Determination Analysis; D013997:Time Factors
chemical_list: D000166:Acridines; D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D000167:Acriflavine