Combined methods for assessment of neurotoxicity: testing of new nonionic radiographic media.
A battery of tests for toxicity assessment of new contrast media is reviewed. Cell toxicity in a protozoan culture (Blepharisma americanum); lethal dose determination in mice; EEG Spectral analysis following cisternography in rabbits; ED50 and LD50 determination by intra- and pericerebral injection in rats; intracisternal injection in chronically cannulated, nonanesthetized rats; and aversive conditioning by intracisternal injection in rats were found complementary. By these methods, novel nonionic media phenyl-sugar-ether DS-1-132 and DL-2-99 were found comparable to iopamidol, while all three compounds were superior to metrizamide.
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002490:Central Nervous System / Q000187:drug effects*; D002946:Cisterna Magna / Q000187:drug effects; D003287:Contrast Media / Q000008:administration & dosage / Q000633:toxicity*; D004353:Drug Evaluation, Preclinical; D004569:Electroencephalography; D056890:Eukaryota / Q000187:drug effects / Q000254:growth & development; D007276:Injections, Intraventricular; D007928:Lethal Dose 50; D051379:Mice; D009192:Myelography / Q000379:methods*; D011817:Rabbits; D051381:Rats
D003287:Contrast Media