Arylsulfatase from Pseudomonas sp. strain C12B: purification to homogeneity, immunological analysis, and physical properties.
Arylsulfatase was purified 219-fold from Pseudomonas sp. strain C12B. The final preparation was homogeneous by electrophoretic and immunological analysis. The enzyme is a monomer of molecular weight about 51,000, with a Stokes radius of 3.0 X 10(-7) cm, a frictional ratio of 1.2, and a sedimentation coefficient of 4.1S.
D001192:Arylsulfatases / Q000302:isolation & purification*; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002621:Chemistry; D008970:Molecular Weight; D011549:Pseudomonas / Q000201:enzymology*; D013429:Sulfatases / Q000302:isolation & purification*
D013429:Sulfatases; D001192:Arylsulfatases