Steroid transformation with immobilized microorganisms. VI. The reverse reaction of steroid-1-dehydrogenases from different micoorganisms in immobilized state.
Whole cells of Nocardia erythropolis, N. opaca and Mycobacterium phlei containing 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase activities were immobilized by adsorption on DEAE-cellulose and silica and by entrapment in polyacrylamide gel. The obtained biocatalysts were used in an anaerobic continuous column process to transform 1,4-dien-3-oxo-steroids into 4-en-3-oxo- steroids. The half life of steroid-1(2)-reductase activity of the N. erythropolis was found to be up to 15 days. The DEAE-cellulose-adsorbed cells showed a slightly higher relative activity than free cells. The influence of substrate concentration and the action of dimethylformamide were also investigated.
D002784:Cholesterol / Q000378:metabolism; D004126:Dimethylformamide / Q000494:pharmacology; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005285:Fermentation; D006207:Half-Life; D008777:Methyltestosterone / Q000378:metabolism; D009161:Mycobacterium / Q000201:enzymology*; D009168:Mycobacterium phlei / Q000201:enzymology*; D009615:Nocardia / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism*; D012855:Sitosterols / Q000378:metabolism
D004800:Enzymes, Immobilized; D012855:Sitosterols; D004126:Dimethylformamide; D002784:Cholesterol; D010088:Oxidoreductases; C022656:3-oxosteroid delta(1) dehydrogenase; D008777:Methyltestosterone