An enzyme-linked immunoassay for pepsinogen in sheep plasma.
An enzyme-linked immunosorbent assay for pepsinogen in sheep plasma is described. The assay requires only 20 microliter of plasma. As little as 250 ng pepsinogen/ml plasma can be measured using antibody-coated polystyrene tubes and if "MicroElisa" plates are used 50 ng pepsinogen/ml plasma can be assayed. The method is more specific, more sensitive, uses less samples, has lower blank values than the present widely-used spectrophotometric method and could easily be automated.
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D010435:Pepsinogens / Q000097:blood*; D012756:Sheep / Q000097:blood*; D013053:Spectrophotometry
D010435:Pepsinogens