Determination of the major metabolite of phentolamine in human plasma and urine by high-performance liquid chromatography.
A reversed-phase, high-performance liquid chromatographic method using UV detection is described for the assay of the major metabolite of phentolamine in plasma and urine before or after enzymatic hydrolysis. Plasma is deproteinized with methanol. The sensitivity limit is 200 ng/ml using 150-microliters samples. Urine is either diluted with water or purified after enzymatic hydrolysis. Concentrations down to 2--3 micrograms/ml could be quantified with acceptable precision. This method was applied to plasma and urine samples from subjects given phentolamine.
D001192:Arylsulfatases; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005966:Glucuronidase; D006801:Humans; D006868:Hydrolysis; D008832:Microchemistry; D010646:Phentolamine / Q000097:blood* / Q000652:urine
D001192:Arylsulfatases; D005966:Glucuronidase; D010646:Phentolamine