Excitation under the scanning electron microscope of DNA-associated fluorescence from chicken erythrocyte nuclei, polytene chromosomes and adenovirus 2 virions.
Biological structures not seen by conventional light microscopy, such as longitudinal striations in polytene chromosomes, and, at the limit of sensitivity, virions of adenovirus 2, have been detected via DNA-associated fluorescence excited under the scanning electron microscope. The maximum sensitivity realized, about 1 detected photon per 700 base pairs, falls short by about an order of magnitude of that required to achieve, in unreplicated specimens, the 2 nm intrinsic resolution of the method. A combination of D2O-H2O substitution with freeze-drying provides the best unquenching procedure found for in situ DNA. DNA-associated fluorescence for light microscopy can be created by moderate exposure of the specimen in the electron microscope.
D000260:Adenoviruses, Human / Q000648:ultrastructure*; D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D002645:Chickens / Q000097:blood; D002875:Chromosomes / Q000648:ultrastructure*; D004247:DNA / Q000032:analysis*; D004912:Erythrocytes / Q000648:ultrastructure; D005453:Fluorescence; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D014771:Virion / Q000648:ultrastructure
D004247:DNA