Cytotoxic and cytolytic action of the subcellular fraction (60-F) of live hemolytic streptococci against Ehrlich ascites carcinoma cells.
The cytotoxic effect of 60-F, a subcellular fraction obtained from 0.5 approximately 0.6 saturation of cell-free extract of live hemolytic streptococci (Su-strain) with ammonium sulfate on Ehrlich ascites carcinoma cells was studied by the assay of incorporation of nucleosides and amino acid, and by the in vitro-in vivo cytocidal experiment. Additionally, the cytolytic effect of 60-F was examined by the assay of RNA and DNA released from the tumor cells. It was found that the tumor cells treated with 60-F did not take up thymidine, uridine, or leucine, that 60-F eradicated the transplantability of tumor cells in mice, and that 60-F released RNA but not DNA from the tumor cells.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology* / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D004273:DNA, Neoplasm / Q000378:metabolism; D005260:Female; D051379:Mice; D009361:Neoplasm Invasiveness; D012334:RNA, Neoplasm / Q000378:metabolism; D013291:Streptococcus / Q000276:immunology*; D013347:Subcellular Fractions / Q000276:immunology
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm