title: [Isolation and characterization of microorganisms with mannanase activity].
abstract: Twenty-three strains of soil microorganisms synthetizing extracellular mannanase were isolated on the medium containing baker's yeast mannan as the sole carbon source. Ten most active strains were identified with respect to their genera. These microorganisms proved capable to hydrolyze mannans, whose mannopyranose units were bound by alpha-1,2-and alpha-1,6-bonds. In addition, 5 of these strains were able to hydrolyze mannans whose mannose residues were bound by alpha-1,3-bond, and 2 strains could synthetize mannanase active towards yeast beta-mannane with 1,3 and 1,4 bonds. The strains tested showed a very low, if any, lytic activity towards yeast Saccharomyces and Candida. This gives evidence that mannanase is an enzyme unable to perform independently lysis of the yeast cell wall.
mesh_terms: D000190:Actinomyces / Q000201:enzymology; D001173:Arthrobacter / Q000201:enzymology; D001407:Bacillus / Q000201:enzymology; D001419:Bacteria / Q000201:enzymology* / Q000302:isolation & purification; D005417:Flavobacterium / Q000201:enzymology; D008361:Mannosidases / Q000032:analysis*; D011549:Pseudomonas / Q000201:enzymology; D012988:Soil Microbiology; D013045:Species Specificity; D044902:beta-Mannosidase
chemical_list: D008361:Mannosidases; D044902:beta-Mannosidase
vernacular_title: Vydelenie i kharakteristika amikroorganozmov s mannanaznoi aktivnost'iu.