Template-specific inhibitor for DNA polymerase isolated from cauliflower inflorescence.
A novel inhibitory factor which greatly inhibits DNA polymerase activity was isolated from the apical portion of the cauliflower inflorescence during purification of DNA polymerases. It can be adsorbed on a DEAE-cellulose column, but not on CM-Sephadex or DNA-cellulose. The factor exclusively inhibits the incorporation of [3H]dTTP into DNA when poly(rA, dT10) is used as the template primer, but not when activated DNA, heat-denatured DNA or native DNA is used as a template. The concentration of the factor in the reaction medium required for 50% inhibition is approx. 8 microgram/ml. The factor is heat-stable, is inactivated by trypsin, and has a maximum ultraviolet absorption at 278 nm. The molecular weight was estimated as 2500-3000 by Sephadex gel chromatography.
D002848:Chromatography, DEAE-Cellulose; D004247:DNA / Q000378:metabolism; D008970:Molecular Weight; D019384:Nucleic Acid Synthesis Inhibitors; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology; D011067:Poly dA-dT / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013698:Templates, Genetic; D013942:Thymine Nucleotides / Q000378:metabolism
D019384:Nucleic Acid Synthesis Inhibitors; D010936:Plant Extracts; D013942:Thymine Nucleotides; D011067:Poly dA-dT; D004247:DNA; C024157:thymidine 5'-triphosphate