Effects of ionizing radiation on the polymerization of microtubules in vitro.
Exposure of phosphocellulose-purified 6S tubulin to ionizing radiation results in a reduction or loss in its ability to participate in polymerization. Evidence is presented which correlates this loss in ability to polymerize with a reduction in the number of titratable sulfhydryl groups and a lowered affinity for guanosine triphosphate.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002417:Cattle; D003037:Cobalt Radioisotopes; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000378:metabolism; D007700:Kinetics; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008870:Microtubules / Q000528:radiation effects* / Q000648:ultrastructure; D011485:Protein Binding; D014404:Tubulin / Q000378:metabolism / Q000528:radiation effects*
D003037:Cobalt Radioisotopes; D046911:Macromolecular Substances; D014404:Tubulin; D006160:Guanosine Triphosphate; D004228:Dithionitrobenzoic Acid