The effect of changing extracellular osmolality on water transport in the human red blood cell as measured by the cell water residence time and the activation energy of water transport.
A pulse NMR technique employing low extracellular Mn2+ concentrations has been used in following the effect of variations in extracellular osmolality on water transport through the human red blood cell membrane. We report results including the effect of osmolality on the cell water lifetime (tau a) and, for the first time, the effect on the proton spin-spin relaxation of the intracellular water (T2a) and the activation energy for the water transport process. Current results are encouraging in correlating the effects seen in this study with suspected membrane functional changes occurring in both in vivo and in vitro aging and during in vitro preservation attempts.
D000328:Adult; D001692:Biological Transport; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008345:Manganese; D009994:Osmolar Concentration; D013816:Thermodynamics; D014867:Water / Q000378:metabolism*
D014867:Water; D008345:Manganese