High-pressure liquid chromatographic determination of sulfamethazine residues in beef tissues.
A high-pressure liquid chromatographic procedure for the determination of sulfamethazine residues in beef tissues is presented. Tissue samples are extracted and analyzed utilizing normal-phase chromatography and a UV detector at 254 nm. Primary and confirmatory identification and quantitative determination are provided at the tolerance limit of 0.1 ppm of sulfamethazine in tissues. Recoveries obtained from liver, fat, kidney, and muscle tissues were 69, 82, 77, and 76%, respectively, when spiked at the tolerance limit.
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D013418:Sulfamethazine / Q000032:analysis* / Q000378:metabolism
D013418:Sulfamethazine