Rapid intracerebroventricular injection assisted by an automatic syringe.
Rapid intracerebroventricular (icv) injections in the conscious mouse are described, using a small volume gas-tight syringe with repeating dispenser device (Hamilton Bonadoz, Switzerland). The device gives reproducibility within 2% of chosen volume, while facilitating rapid icv injections to groups of animals. The technique is used to demonstrate in the mouse the antinociceptive activity of clonidine, using the tail-immersion test.
D000698:Analgesia; D000818:Animals; D003000:Clonidine / Q000008:administration & dosage / Q000494:pharmacology; D007276:Injections, Intraventricular / Q000295:instrumentation* / Q000379:methods; D051379:Mice; D013594:Syringes
D003000:Clonidine