Phospholipid metabolism in Mycobacterium smegmatis ATCC 607 grown at 37 degrees and 27 C degrees C.
The rate of synthesis and degradation of phospholipids in Mycobacterium smegmatis ATCC 607, grown at 27 degrees C and 37 degrees C was studied incorporation of 32P into phospholipids and chase of radioactivity of the pulse-labelled phospholipids. A relatively low rate of synthesis and degradation of phospholipids in cells growth at 27 degrees C was observed as compared to those grown at 37 degrees C. Phosphatidylethanolamine (PE) had the maximum turnover at 37 degrees C. However, at 27 degrees C, cardiolipin (CL) showed a turnover rate higher than PE. Phosphatidylinositol mannosides (PIMs) were metabolically more active at 37 degrees C than at 27 degrees C. The differences in metabolic activity of the phospholipids at the two temperatures have been discussed.
D002308:Cardiolipins / Q000378:metabolism; D007700:Kinetics; D008362:Mannosides / Q000378:metabolism; D009161:Mycobacterium / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D013696:Temperature
D002308:Cardiolipins; D008362:Mannosides; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D010743:Phospholipids; C034154:phosphatidylinositol mannoside