Tritiated thymidine incorporation does not measure DNA synthesis in ribavirin-treated human cells.
When the incorporation of tritiated thymidine into acid insoluble material was measured, ribavirin appeared to be a potent inhibitor of DNA synthesis in KB cells and human lymphocytes. Inhibition was nearly 100-fold less, however, when DNA synthesis was measured by incorporation of phosphorus-32-labeled phosphate or by DNA fluorescence. The potent inhibition detected by incorporation of tritiated thymidine into DNA actually was the result of a potent effect on the labeling of deoxythymidine triphosphate, not on the synthesis of DNA.
D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication / Q000187:drug effects*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D012254:Ribavirin / Q000494:pharmacology*; D012263:Ribonucleosides / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism*; D013942:Thymine Nucleotides / Q000096:biosynthesis
D010710:Phosphates; D012263:Ribonucleosides; D013942:Thymine Nucleotides; D012254:Ribavirin; D004247:DNA; C024157:thymidine 5'-triphosphate; D013936:Thymidine