Cytotoxicity of temporarily adherent human mononuclear blood cells. 1. Cytotoxic abilities of cells after detachment from culture wells.
Monolayers of freshly isolated peripheral blood monocytes are contaminated with 5-10% non-monocytic cells. These cells detach during the first day of in vitro culture, concomitant with a disappearance of the strong cytostatic and cytolytic activity of freshly isolated monocytes. The detached cells, mostly non-monocytic cells, exerted cytostasis and cytolysis on the human natural killer (NK) sensitive cell line K-562. The lytic activity was sensitive to trypsin treatment. The kinetics of cytolysis was NK-like, with maximal 51Cr-release within 4-8 hours of co-culture, and with a high degree of methyl-3H-thymidine release during the first 24 hour of co-culture, and with a high degree of methyl-3H-thymidine release during the first 24 hours of co-culture. It is suggested that these cells may be a loosely adherent subpopulation of mononuclear cells with NK-like properties.
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D007942:Leukemia, Experimental / Q000276:immunology; D007962:Leukocytes / Q000145:classification* / Q000276:immunology; D008222:Lymphokines / Q000494:pharmacology; D009000:Monocytes / Q000276:immunology*; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
D008222:Lymphokines; D014357:Trypsin