Cytocidal effect of alpha-methylornithine, a competitive inhibitor of ornithine decarboxylase, on ehrlich ascites tumor cells in vivo.
The antiproliferative effect of alpha-methylornithine, an inhibitor of the initial and rate-limiting step in polyamine biosynthesis, was studied on Ehrlich ascites tumor cells grown in vivo. To assess drug-induced cell lethality, the loss of 125I from mice harboring 5-[125I] iodo-2'-deoxyuridine-labeled mouse tumor cells was determined during the course of alpha-methylornithine treatment. Mice that received the drug showed a significant increase in their rate of 125I excretion as compared to controls. This suggests that alpha-methylornithine kills an additional fraction of tumor cells beyond the fraction normally dying.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors*; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D008297:Male; D051379:Mice; D009952:Ornithine / Q000031:analogs & derivatives* / Q000494:pharmacology; D065108:Ornithine Decarboxylase Inhibitors
D000970:Antineoplastic Agents; D065108:Ornithine Decarboxylase Inhibitors; C025025:alpha-methylornithine; D009952:Ornithine; D002262:Carboxy-Lyases