[New ultrasonic method for studying the composition and properties of multienzyme systems: enzymes of the cellulase complex].
A new ultrasonic method for determination of the composition and properties of individual components of multienzyme systems without their resolution has been developed. The method is based on a determination of the pH-profiles of the first order rate constants for inactivation of the enzymatic components in a complex by ultrasonic cavitation. The method was used for studying a cellulase complex from Geotrichum candidum. It was shown that the cellulase complex contains at least four cellulolytic enzymes, i. e. endoglucanase, exoglucosidase, cellobiase and aryl-beta-glucosidase, which differ in their pK values for the ionogenic groups controlling the pH-profiles of ultrasonic inactivation and in the inactivation rate constants at the plateau of a pH-rate profile.
D002480:Cellulase / Q000378:metabolism*; D005848:Geotrichum / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008722:Methods; D009097:Multienzyme Complexes / Q000378:metabolism*; D014465:Ultrasonics
D009097:Multienzyme Complexes; D002480:Cellulase
Novyĭ ul'trazvukovoĭ metod izucheniia sostava i svoĭstv polifermentnykh sistem: fermenty tselliulaznogo kompleksa.