Change in the expression of collagen genes in dividing and nondividing chondrocytes.
Chondrocytes were isolated from the sterna of 17-day-old chick embryos by enzyme digestion. Rapid proliferation of chondrocytes was achieved in the presence of chick serum (10%, v/v). Addition of either hydroxyurea (10(-3) M) or cytosine arabinoside (10 microgram/ml) to the culture medium was used to arrest growth of cells. Under both conditions, however, a rather fast switch of synthesis from type II to type I collagen was observed. This suggests that the loss of the differentiated state of chondrocytes in culture is not necessarily bound to mitosis.
D000818:Animals; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003094:Collagen / Q000096:biosynthesis*; D003561:Cytarabine / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D006918:Hydroxyurea / Q000494:pharmacology
D003561:Cytarabine; D003094:Collagen; D006918:Hydroxyurea