title: Interaction of acrylonitrile with hepatic microsomes of rats and men.
abstract: Acrylonitrile (AN) showed spectral interaction with hepatic microsomes from mouse, rat and man. Whereas human and rat liver microsomes resulted in ligand AN-binding spectra, mouse liver microsomes behaved differently. Hepatic microsomes from phenobarbital-treated mice with AN showed a type I substrate binding; ligand spectra are recorded with microsomes from benzo[a]pyrene-treated mice. Microsomes from untreated control mice showed a mixed type behaviour.
mesh_terms: D000181:Acrylonitrile / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D019388:Cytochrome P-450 CYP1A2; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008024:Ligands; D051379:Mice; D008810:Mice, Inbred C57BL; D008862:Microsomes, Liver / Q000378:metabolism*; D009570:Nitriles / Q000378:metabolism*; D051381:Rats; D013045:Species Specificity; D013053:Spectrophotometry
chemical_list: D003580:Cytochromes; D008024:Ligands; D009570:Nitriles; D003577:Cytochrome P-450 Enzyme System; C017170:Cyp1a2 protein, rat; D019388:Cytochrome P-450 CYP1A2; D000181:Acrylonitrile