Some cultural conditions that control production of verrucarin J, a cytotoxic metabolite of Stachybotrys chartarum.
A suitable chemically defined culture medium was selected and some optimal conditions for the biosynthesis of the highly cytostatic and antifungal compound verrucarin J were reported. Medium of the following composition was favourable for the production of verrucarin J by Stachybotrys chartarum: sucrose, 50; NaNO3, 2.0; KH2PO4, 1.0; MgSO4, 0.5; KCl, 0.5; leucine, 1.0 and FeSO4, 0.01 (g/l of distilled water). Biosynthesis of verrucarin J was maximal (11.8 mg/l) at pH 6.5-7.0 and after incubation for 14 days at 25 degrees C.
D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis*; D050260:Carbohydrate Metabolism; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D003904:Mitosporic Fungi / Q000254:growth & development*; D009584:Nitrogen / Q000378:metabolism; D013191:Stachybotrys / Q000254:growth & development*; D014255:Trichothecenes / Q000096:biosynthesis
D000903:Antibiotics, Antineoplastic; D003470:Culture Media; D014255:Trichothecenes; C035445:verrucarin J; D009584:Nitrogen