title: Shape and functional properties of human platelets washed with acid citrate.
abstract: Acid citrate pH 6.5 protects human platelets from activation during washing at room temperature. Platelets suspended in media containing citrate pH 6.5 have an extra-flat disc shape. Citrate at pH 6.5 also inhibits the ADP-induced shape change. When warmed to 37 degrees C and suspended into the test medium of pH 7.4, the washed platelets aggregate with low concentrations of ADP or epinephrine in the presence of fibrinogen and Ca2+. The morphological and functional quality of platelet concentrates (6 X 10(6) microliter-1) stored in acid citrate pH 6.5 is maintained for at least 6 h at room temperature.
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000166:cytology* / Q000502:physiology*; D001800:Blood Specimen Collection; D002118:Calcium / Q000494:pharmacology; D002951:Citrates / Q000494:pharmacology*; D004837:Epinephrine / Q000494:pharmacology; D005340:Fibrinogen / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010974:Platelet Aggregation / Q000187:drug effects
chemical_list: D002951:Citrates; D000244:Adenosine Diphosphate; D005340:Fibrinogen; D002118:Calcium; D004837:Epinephrine