The use of radioimmunoassay to study the formation and disappearance of O6-methylguanine in mouse liver satellite and main-band DNA following dimethylnitrosamine administration.
A sensitive radioimmunoassay for O6-methyldeoxyguanosine has been developed, permitting the analysis of microgram amounts of DNA. This technique has been used in the study of the formation and removal of O6-methylguanine in mouse liver satellite and main-band DNA. The results indicate a reduced extent of O6-methylguanine formation in satellite DNA but similar rates of its removal from both classes of DNA.
D000478:Alkylation; D000818:Animals; D004260:DNA Repair; D004276:DNA, Satellite / Q000378:metabolism*; D004128:Dimethylnitrosamine / Q000633:toxicity*; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008760:Methylguanidine / Q000276:immunology; D051379:Mice; D011817:Rabbits; D011863:Radioimmunoassay
D004276:DNA, Satellite; D008760:Methylguanidine; D006147:Guanine; C008449:O-(6)-methylguanine; D004128:Dimethylnitrosamine