[Biosynthesis of collagen in a cell-free system from wheat germ on poly(A)-RNA isolated from membrane-bound polyribosomes of chicken embryos].
The total protein-synthesizing and collagen-synthesizing activities of poly(A)-containing RNA from the membrane-bound polyribosomes of chicken embryos were studied under various conditions in a cell-free wheat germ system. The optimal concentrations of poly(A)-RNA, mono- and bivalent cations and spermine necessary for the translation of collagen and non-collagen proteins were determined. It was shown that within a wide range of K+ and Mg2+ concentrations in a cell-free system the poly(A)-RNAs studied are very actively involved in the translation of non-collagen proteins. At the same time the bacterial collagenase-cleavable polypeptides synthesized on poly(A)-RNA from the membrane-bound polyribosomes were identified only after addition of polyamine -- spermine (40 mk/M) to the system. In this case the relative level of collagen polypeptide synthesis remains constant within a wide range of concentrations of mRNA, K+ and Mg2+ in the system. Collagen-synthesizing activity of poly(A)-RNA from membrane-bound polyribosomes in a heterologous cell-free system as compared to that of membrane-bound polyribosomes in a homologous system showed that these polyribosomes can be effectively used for isolation of collagen mRNA.
D000818:Animals; D002642:Chick Embryo; D003094:Collagen / Q000096:biosynthesis*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D010944:Plants / Q000378:metabolism; D011061:Poly A / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D014176:Protein Biosynthesis; D012335:RNA, Ribosomal / Q000378:metabolism*; D013096:Spermine / Q000494:pharmacology; D014908:Triticum / Q000378:metabolism
D012335:RNA, Ribosomal; D011061:Poly A; D013096:Spermine; D003094:Collagen; D008274:Magnesium; D011188:Potassium
Biosintez kollagena v beskletochnoĭ sistem iz zarodysheĭ pshenitsy na poli (A)-RNK, vydelennoĭ iz membranosviazannykh poliribosom kurinykh em rionov.