A simple chromatographic technique for the estimation of urinary levels of normetadrenaline and metadrenaline.
A simple, but effective, procedure is described for the concurrent estimation of normetadrenaline (NMA) and metadrenaline (MA) in urine. The 3-methoxycatecholamines are adsorbed by a cation exchange resin, desorbed, separated by paper chromatography, and finally estimated spectrophotometrically after oxidation to vanillin. The mean (+/- 2 standards deviations) urinary outputs of NMA and MA from 123 normals (80 males, 43 females) in 24 hours was 2.15 +/- 1.04 and 1.79 +/- 0.96 mu mol respectively. There was no significant difference in the 24-hour urinary outputs of NMA between the male and female groups. However, the output of MA from the males was significantly higher than that from the females.
D000328:Adult; D002854:Chromatography, Paper / Q000379:methods; D004837:Epinephrine / Q000031:analogs & derivatives*; D005260:Female; D006801:Humans; D008297:Male; D008676:Metanephrine / Q000302:isolation & purification / Q000652:urine*; D009647:Normetanephrine / Q000302:isolation & purification / Q000652:urine*; D012016:Reference Values; D012737:Sex Factors; D013056:Spectrophotometry, Ultraviolet
D009647:Normetanephrine; D008676:Metanephrine; D004837:Epinephrine