title: A method for the rapid quantitation of methylated hepatic DNA-purines using high pressure liquid chromatography.
abstract: An isocratic high pressure liquid chromatography system that can be employed for the separation and quantitation of four of the major methylated purines commonly found in DNA after exposure to carcinogenic/mutagenic alkylating agents (1-methyladenine, 3-methyladenine, O6-methylguanine and 7-methylguanine) is presented in this paper. Data from experiments in which rats were treated with methyl-N-nitrosourea have been used to illustrate the applicability of the method. The amounts of the methylated bases observed were consistent with values reported in the literature.
mesh_terms: D000477:Alkylating Agents / Q000633:toxicity*; D000818:Animals; D002843:Chromatin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004247:DNA / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D008297:Male; D008745:Methylation; D011687:Purines / Q000032:analysis*; D051381:Rats
chemical_list: D000477:Alkylating Agents; D002843:Chromatin; D011687:Purines; D004247:DNA