Screening and characterization of membrane damaging effects in tissue culture.
A leakage test system for specific detection, measurement and characterization of membrane damage in human lung fibroblasts is discussed. Examples of applications with various types of substances are presented, i.e. tobacco smoke compounds; animal, plant and microbial cytolysins; membrane damaging antibiotics.
D000900:Anti-Bacterial Agents / Q000633:toxicity; D002463:Cell Membrane Permeability / Q000187:drug effects*; D046508:Culture Techniques; D003603:Cytotoxins / Q000633:toxicity; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008168:Lung / Q000187:drug effects
D000900:Anti-Bacterial Agents; D003603:Cytotoxins