Thymidine uptake by chicken kidney cells parasitized by Eimeria tenella: evidence for a diffusable mediating substance.
The pattern of DNA synthesis in chicken kidney (CK) cells parasitized by Eimeria tenella was altered; a 2-fold increase in the uptake of [3-H]-thymidine occurred in these cells compared with that shown by the controls. This difference was significantly different at 16 h post-inoculation (p.i.) and reached a peak at 20 h p.i. Non-parasitized cells in co-culture with parasitized cells showed a level of incorporation intermediate between that of the parasitized and non-parasitized monocultures. It was shown that this stimulation was due to a factor released into the medium by the parasite or the parasitized cell. A possible course of events leading to induced and prolonged DNA synthesis by the host cell and the significance of this change in the life-cycle of the parasite is discussed.
D000818:Animals; D002478:Cells, Cultured / Q000378:metabolism*; D002645:Chickens; D004247:DNA / Q000096:biosynthesis; D004539:Eimeria / Q000254:growth & development* / Q000378:metabolism; D006790:Host-Parasite Interactions; D007668:Kidney; D013936:Thymidine / Q000378:metabolism*
D004247:DNA; D013936:Thymidine