Chemically defined medium enhances bioelectric activity in mouse spinal cord-dorsal root ganglion cultures.
Co-cultures of mouse spinal cord with dorsal root ganglion (DRG) cultures were grown either in horse serum (HS)-supplemented medium or in a serum-free, chemically defined medium (CDM). The cytoarchitecture of cord--DRG explants was fully retained in CDM, with little or no distortion due to flattening of the explant, as is invariably observed in HS-supplemented cultures. Functional properties such as bioelectric activity and DRG--spinal cord interconnectivity were well sustained in CDM.
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004553:Electric Conductivity; D005727:Ganglia, Spinal / Q000166:cytology / Q000502:physiology*; D051379:Mice; D013116:Spinal Cord / Q000166:cytology
D003470:Culture Media