Morphogenesis of vaccinia virus in the process of envelopment as observed by freeze-etching electron microscopy.
The morphogenesis of vaccinia virus was studied during its synthesis in HeLa cells. Freeze-etching electron microscopy demonstrated intramembrane granules attached to convex faces, a granule-free area characterized by eccentric location and smooth appearance, and an eccentric nucleoid structure occasionally associated with a membranous structure. It is postulated that the granule-free area may be a precursor of the nucleoid envelope.
D003460:Crystallization; D005614:Freeze Fracturing; D006367:HeLa Cells; D006801:Humans; D008566:Membranes / Q000648:ultrastructure; D008854:Microscopy, Electron; D008961:Models, Structural; D009024:Morphogenesis; D014616:Vaccinia virus / Q000254:growth & development* / Q000648:ultrastructure