[New progress in detection of antigens from human ejaculate in relationship with human sterility (extraction with N-cetylpyridinium chloride) (author's transl)].
In an attempt to obtain certain sub-surface spermatozoa antigens, human spermatozoa were treated with N-cetylpyridinium chloride in basic buffer, The extract was further ultrasonated and separated with gel chromatography over a Sephadex--G--100 column. The obtained fractions were lyophilized and tested against human sperm agglutinating and sperm immobilizing sera, in order to detect their antigenicity. Positive and negative controls revealed intensive precipitations and no precipitations respectively. Fraction A4 reacted intensively with sperm immobilizing sera. The final objectives of these sperm antigens solubilization studies are twofold. On the one hand we are looking for a method to lower sperm antibodies titres in sterile females, and on the other hand we are seeking an immunological contraceptive method.
D000941:Antigens / Q000032:analysis; D002594:Cetylpyridinium; D002850:Chromatography, Gel; D006801:Humans; D008297:Male; D011726:Pyridinium Compounds; D013073:Sperm Agglutination; D013078:Sperm Immobilizing Agents / Q000378:metabolism; D013094:Spermatozoa / Q000276:immunology*
D000941:Antigens; D011726:Pyridinium Compounds; D013078:Sperm Immobilizing Agents; D002594:Cetylpyridinium
Nuevos aportes en la deteccion de antígenos del eyaculado humano vinculados con la esterilidad conyugal (extracción con N-cetylpiridinium chlorid).