Automated Jendrassik-Grof method for measurement of bilirubin in serum with the Greiner Selective Analyzer (GSA II D), and comparison with the method involving diazotized 2,4-dichloroaniline.
We automated the Jendrassik-Grof method for measurement of bilirubin with the Greiner Selective Analyzer GSA II D. Comparison with the manual Jendrassik-Grof method showed a highly significant correlation coefficient of 0.994 for total and 0.980 for "direct" bilirubin measurement. The automated method showed good precision and was linear to 667 mu mol/L, the highest concentration tested. Addition of hemolysate caused a greater effect on the measurement of direct bilirubin than on total bilirubin. The inhibitory effect on measurement of total bilirubin, though small, was statistically significant at hemoglobin concentrations greater than 2 g/L. Comparison with the method involving diazotized 2,4-dichloroaniline showed the Jendrassik-Grof method to be relatively less affected by hemolysis and turbidity in the measurement of total bilirubin.
D001322:Autoanalysis; D001663:Bilirubin / Q000097:blood*; D003979:Diazonium Compounds; D006454:Hemoglobins / Q000032:analysis; D006461:Hemolysis; D006801:Humans; D007202:Indicators and Reagents; D013053:Spectrophotometry / Q000379:methods
D003979:Diazonium Compounds; D006454:Hemoglobins; D007202:Indicators and Reagents; C029901:2,4-dichlorobenzenediazonium; D001663:Bilirubin