Osmotic shrinkage of giant egg-lecithin vesicles.
Osmotic shrinkage of giant egg-lecithin vesicles was observed by phase-contrast microscopy. The vesicles remained or became spherical when shrinking. Small and thick-walled vesicles formed visible fingers attached to the sphere. The water permeability of the single bilayer was found to be 41 micrometers/s. A variety of observations indicate that osmosis induces a parallel lipid flow between the monolayers of the bilayer, leading to a strong positive spontaneous curvature. They also suggest the formation of mostly submicroscopic daughter vesicles. The estimated coupling constant, 2 . 10(-6) mol/mol, is large enough to be biologically significant.
D008051:Lipid Bilayers; D009995:Osmosis; D010539:Permeability; D010713:Phosphatidylcholines; D014867:Water
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D014867:Water