A lipoprotein independent assay for human serum lecithin-cholesterol acyltransferase.
A method has been developed for estimation of human serum lecithin-cholesterol acyltransferase free of interference by endogenous lipoproteins. Precipitation of serum low and very low density lipoproteins by sodium phosphotungstate and magnesium chloride results in complete recovery of lecithin-cholesterol acyltransferase activity in the supernatant. One microliter of the supernatant can be accurately assayed with a highly efficient substrate containing phosphatidylcholine-cholesterol vesicles and apo-high density lipoproteins (HDL), with no interference from endogenous HDL or residual precipitation reagents. Serum levels of the enzyme were found to be reduced in patients with parenchymal liver disease, renal disease, gastrointestinal tumors and anemias.
D004194:Disease / Q000201:enzymology; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008274:Magnesium; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood*; D010772:Phosphotungstic Acid
D008074:Lipoproteins; D010772:Phosphotungstic Acid; D007862:Phosphatidylcholine-Sterol O-Acyltransferase; D008274:Magnesium