Metabolism of zinc and copper in the neonate: accumulation of Cu in the gastrointestinal tract of the newborn rat.
1. The concentration of copper in the rat intestine was found to increase rapidly after birth to a maximum greater than 140 microgram/g wet weight at 2 d of age and then to decline, at first slowly to 90 microgram/g wet weight on day thirteen and then rapidly to 40 microgram/g and 3 . 4 microgram/g wet weight on the 15th and 19th day respectively. The intestinal concentration of Zn, which doubled between 1 d prepartum and 2 d post partum, also fell slowly until 10 d of age, but thereafter remained constant. 2. From the 2nd to the 15th day post partum approximately 60% of the total Cu and 50% of the total zinc in the intestine was located in the soluble fraction of the tissue. Most of the Zn in this fraction was bound by proteins of molecular weights greater than 13700 daltons, whereas most of the Cu was present as an extremely polydisperse complex of lower molecular weight. This complex in the intestine of the 5-d-old rat, in contrast with the soluble proteins of higher molecular weight, did not incorporate either 3H or 35S within 4 h of the administration of L-[4,5-3H]leucine and L-[35S]cystine. 3. The loss of Cu from the intestine between the 13th and 15th day of post-natal age occurred mainly from this complex and was accompanied by the transient appearance of Cu in a fraction of low molecular weight. 4. At 21 d of age the soluble fraction of the intestine contained only a small amount of Cu. This was distributed between two protein fractions, one of which contained Zn and appeared to be a metallothionein. 5. The results are discussed in relation to the control of Zn and Cu absorption.
D000375:Aging; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D003300:Copper / Q000378:metabolism*; D004064:Digestive System / Q000378:metabolism*; D008668:Metallothionein / Q000378:metabolism; D051381:Rats; D015032:Zinc / Q000378:metabolism*
D003300:Copper; D008668:Metallothionein; D015032:Zinc