Properties of pancreatic zymogen granules studied by quasi-elastic light scattering.
Physico-chemical properties of isolated zymogen granules of the mouse pancreas were studied by means of quasi-elastic light scattering. The average diameter of the granules in 0.3 M sucrose was found to be 1.1 + or minus 0.1 micrometer from the correlation time of intensity fluctuation of the scattered light. The average diameter altered depending on the osmolality of the medium in a manner that the alteration was smaller than that expected from the van't Hoff relation. Aggregation of the granules induced by the increase of Ca2+ concentration or the decrease of pH in the medium was also detected. The aggregation started at a critical level of 1 mM CaCl2 or at pH 5.4.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003594:Cytoplasmic Granules / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008027:Light; D051379:Mice; D009994:Osmolar Concentration; D010179:Pancreas / Q000032:analysis*; D012542:Scattering, Radiation
D002118:Calcium