title: [35S]-methionine labelled polypeptides from HELA cells. Coordinates and percentage of some major polypeptides.
abstract: A total of 1169 [35S]-methionine labelled polypeptides (855-acidic (IEF) and 314 basic (NEPHGE) from 100 asynchronous human HeLa cells have been separated using high resolution two dimensional gel electrophoresis. Beside numbering these spots, we give for the major polypeptides their coordinates (M.W. and relative mobility respect to beta-actin (IEF) or NEPHGE), and percentage of total protein label. A few major polypeptides have been identified.
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells / Q000378:metabolism*; D006801:Humans; D007525:Isoelectric Focusing; D008715:Methionine / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D011506:Proteins; D008715:Methionine