[Feasibility of maintaining the structure and amount of DNA in nuclei of different cells during acid hydrolysis].
Effects of different types of fixation on preservation of DNA structure and amount in human leukocyte and chicken red blood cell nuclei stained according to Feulgen were studied. Regardless of hydrolysis conditions (5N HCl, 22 and 37 degrees C) the best results were obtained with use of a 95% ethanol-glacial acetic acid mixture (3:1) containing 2% of formaldehyde characterized by its independence on the fixation duration (other fixatives require the selection of the optimal fixation time), a satisfactory preservation of the native nuclear shape and of the chromatin structure, and by its ability to fix the maximal amount of reactable, aldehyde groups within DNP during a long period of time. In ethanol fixation, addition to the hydrolyzing solution of 20% of polyethylene glycol 6000 results in a stable plateau on the hydrolysis curve and in an increase of DNA-fuchsin amount by 5-32%.
D000085:Acetates / Q000494:pharmacology; D000818:Animals; D002645:Chickens / Q000097:blood*; D004247:DNA / Q000032:analysis*; D003852:Deoxyribonucleoproteins / Q000032:analysis; D004912:Erythrocytes / Q000032:analysis*; D000431:Ethanol / Q000494:pharmacology; D005404:Fixatives / Q000494:pharmacology*; D005557:Formaldehyde / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D007962:Leukocytes / Q000032:analysis*; D008297:Male; D009690:Nucleic Acid Conformation; D011092:Polyethylene Glycols / Q000494:pharmacology
D000085:Acetates; D003852:Deoxyribonucleoproteins; D005404:Fixatives; D005557:Formaldehyde; D000431:Ethanol; D011092:Polyethylene Glycols; D004247:DNA
O vozmozhnosti sokhraneniia struktury i kolichestva DNK v iadrakh razlichnykh kletok pri kislotnom gidrolize.