The selective effect of tunicamycin on the secretion of IgM and IgG produced by the same cells.
Cells of the hybridoma cell line X63/38 C-13 secreting both IgM and IgG have been used in studies on the importance of the carbohydrate moieties of the Ig molecules for their secretion. It has been found that the secretion of the IgM produced in the presence of the glycosylation inhibitor tunicamycin was strongly inhibited, while the secretion of the IgG produced by the same cells was only slightly affected.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005944:Glucosamine / Q000031:analogs & derivatives*; D006023:Glycoproteins; D006822:Hybrid Cells / Q000378:metabolism*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D007930:Leucine / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D051379:Mice; D011487:Protein Conformation; D012995:Solubility; D014415:Tunicamycin / Q000494:pharmacology*
D006023:Glycoproteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014415:Tunicamycin; D007930:Leucine; D005944:Glucosamine; D008358:Mannose