[Measurements of the surface dose for the irradiation of cultured cells with a Co-60 source (author's transl)].
In order to exactly determine the irradiation dose for cultured cells in culture containers (Petri dishes and others), it is necessary to have a detailed knowledge of the depth dose curve in water-equivalent layers near the surface. This curve was determined for Co-60 radiation by means of a window-less liquidity dosemeter. The dosemeter liquid was a solution of ammonium-iron (II) sulfate (solution of Fricke), the extinction of which was measured at 304 nm. Petri dishes were used instead of closed irradiation flasks. The distance QOA was 50 cm for all experiments, the field size was 10 X 10 cm. The depth dose maximum was found in a depth of 5,0 mm which corresponds to a mass of 0,50 g cm-2 relating to the surface. The surface dose was determined to be 39,2% and 106,6% of the maximum dose, respectively. This method can also be applied for closed culture containers (flasks).
D002478:Cells, Cultured / Q000528:radiation effects*; D003037:Cobalt Radioisotopes; D008433:Mathematics; D008722:Methods; D011829:Radiation Dosage
D003037:Cobalt Radioisotopes
Oberflächendosismessungen zur Bestrahlung von Zellkulturen mit einer Co-60-Quelle.