Quantitative analysis of melphalan and its major hydrolysate in patients and animals by reversed-phase high-performance liquid chromatography.
The detection of 4-bis-(2-hydroxyethyl)amino-1-phenylalanine (L-DOH) in blood samples taken from patients after treatment with melphalan [4-bis-(2-chloroethyl)amino-1-phenylalanine, L-PAM] suggests that the quantification of this major hydrolysate of L-PAM can be of considerable importance in L-PAM chemotherapy. A reversed-phase high-performance liquid chromatographic procedure has been developed for the quantitative analysis of both L-PAM and L-DOH in biological samples, with a detection sensitivity of 0.1 ppm. This method provides a distinct separation of L-PAM (retention time 12 min) and L-DOH (retention time 6.5 min), with no interference from the biological background (retention time 1.4--3 min).
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007700:Kinetics; D008297:Male; D008558:Melphalan / Q000031:analogs & derivatives* / Q000097:blood* / Q000378:metabolism; D008832:Microchemistry; D051381:Rats
C030227:4-bis(2-hydroxyethyl)amino-L-phenylalanine; D008558:Melphalan