Chromosomal radiosensitivity of pig leucocytes in relation to sampling time.
Pig blood cultures were used to analyse the sensitivity to X-rays (measured as frequency of induced dicentrics) of lymphocytes sampled at variable times. By using the BrdU-Giemsa method it was possible to identify the lymphocytes that were performing their first division at early (less than 30% of cells in second division), intermediate (30-50% of cells in second or subsequent divisions) and late stages (more than 50% of cells in second or subsequent divisions). No difference was found in the radiosensitivity of these 3 varieties of lymphocyte. It was also observed that: (a) the combination of radiation followed by BrdU treatment did not increase the clastogenic action of X-rays, (b) X-rays in the dose used in our cultures did not increase the frequency of SCEs, and (c) minor changes in culture conditions probably influence the basal frequency of SCEs.
D000818:Animals; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D008214:Lymphocytes / Q000648:ultrastructure; D008938:Mitosis; D013552:Swine / Q000235:genetics*; D013997:Time Factors; D014965:X-Rays