Biochemical studies on McLeod phenotype erythrocytes.
Red cells of the McLeod phenotype in the Kell blood group system have an acanthocytic morphology. The membrane protein composition analyzed on sodium dodecyl-sulfate-polyacrylamide gel electrophoresis, the ATP level and the activities of a large number of intracellular enzymes appear to be normal. Membranes prepared from McLeod red cells incubated with gamma AT[32P] and MgCl2 incorporated twice as much radioactivity into spectrin and also showed a slight elevation of phosphorylation in band 3 protein when compared to membranes from normal cells. Intact normal red cells incubated with carrier-free [32P] incorporated radioactivity into several proteins, with most incorporation in spectrin and band 3 protein. In comparison, McLeod cells incorporated three times more radioactivity into spectrin and band 3 protein but increased phosphorylation also occurred in other, but not all, membrane proteins. Intact McLeod red cells also showed increased phosphorylation of membrane phospholipids, but they incorporated [32P] into intracellular nucleotide phosphates in a normal manner.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001345:Autoradiography; D001789:Blood Group Antigens / Q000276:immunology*; D055598:Chemical Phenomena; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000032:analysis* / Q000201:enzymology; D006801:Humans; D007626:Kell Blood-Group System / Q000276:immunology*; D008565:Membrane Proteins; D010641:Phenotype; D010743:Phospholipids; D010766:Phosphorylation
D001789:Blood Group Antigens; D007626:Kell Blood-Group System; D008565:Membrane Proteins; D010743:Phospholipids; D000255:Adenosine Triphosphate