High-performance liquid chromatographic determination of penicillamine in whole blood, plasma, and urine.
A high-performance liquid chromatographic method for the determination of penicillamine in plasma, whole blood, and urine samples is described. The method uses a commercially available electrochemical detector at a potential of +0.1 V versus the Ag/AgCl reference electrode. This method is selective and sensitive for sulfhydryl compounds. The chromatography separates penicillamine from other endogenous sulfhydryl compounds with a limit of detection for penicillamine in biological samples of ca. 10(07) M.
D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D008832:Microchemistry; D010396:Penicillamine / Q000097:blood* / Q000652:urine; D010949:Plasma / Q000032:analysis
D002021:Buffers; D007202:Indicators and Reagents; D010396:Penicillamine