Mass spectrometric sequence studies of a superoxide dismutase from Bacillus stearothermophilus.
A partial sequence of the manganese-containing superoxide dismutase from Bacillus stearothermophilus has been determined by mass spectrometry. A new fragmentation reaction at N-terminal proline is described and an 'in-chain' fragmentation, also occurring at proline, has been observed. There is some evidence that the latter cleavage may also occur at other residues. Some differences from the classically determined sequence are reported, and the validity of these observations is discussed.
D000595:Amino Acid Sequence; D005591:Chemical Fractionation; D004590:Electrophoresis, Paper; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000302:isolation & purification; D013482:Superoxide Dismutase / Q000032:analysis*
D010446:Peptide Fragments; D013482:Superoxide Dismutase