The carcinogen chromate induces DNA cross-links in rat liver and kidney.
DNA cross-links were found in nuclei isolated from the liver and kidney of rats treated with chromate. A dose-dependent relationship between chromate exposure and total DNA cross-links was determined using the alkaline elution technique. DNA cross-links in kidney were mainly DNA-protein in nature. Chromate also induced a small amount of interstrand DNA cross-links in kidney. Liver nuclei contained protein-associated DNA single strand breaks in addition to DNA-protein cross-links. The organotropic DNA damage induced by chromate is discussed in relation to the carcinogenicity and toxicity of chromium(VI) compounds.
D000818:Animals; D002273:Carcinogens; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D002840:Chromates / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D009928:Organ Specificity; D051381:Rats; D013050:Spectrometry, Fluorescence
D002273:Carcinogens; D002840:Chromates; D004247:DNA