Interaction of liposomes with homologous series of fluorescent berberine derivatives. New cationic probes for measuring membrane potential.
The interaction of liposomes with a series of fluorescent berberine derivatives having different alkyl chain lengths has been investigated. The hydrophobicity of the binding site on the phospholipid membrane increases and mobility decreases with the length of the alkyl chain. If lauryl sulphate micelles are used to bind berberines, the hydrophobicity of the binding site is the same for all derivatives. The dye series represents a model with constant charge and growing lipophilicity. Both electrostatic forces and lipophilicity play an important role in binding. By virtue of the excellent sensitivity of the dyes to medium polarity, berberines prove to be suitable probes for measuring membrane potential, but only in cases when a negative charge is generated in the liposomal interior. The fluorescent response is a linear function of the potential magnitude.
D001599:Berberine / Q000031:analogs & derivatives; D001600:Berberine Alkaloids; D005456:Fluorescent Dyes; D008081:Liposomes; D008564:Membrane Potentials; D011188:Potassium; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013329:Structure-Activity Relationship; D014634:Valinomycin
D001600:Berberine Alkaloids; D005456:Fluorescent Dyes; D008081:Liposomes; D001599:Berberine; D014634:Valinomycin; D011188:Potassium