title: [Complex isolation of intra- and extracellular RNases from Aspergillus clavatus].
abstract: A scheme for isolating guanyl-specific and -nonspecific intracellular and extracellular RNases from the fungus Aspergillus clavatus cultivated in a fermenter has been developed. The scheme involves chromatography on DEAE and CM-celluloses, QAE Sephadex A-50 gel filtration on Sephadex G-75 and ultragel A-44, and crystallization. Using the scheme, four electrophoretically homogeneous enzymes have been purified, two of them being crystalline guanyl-specific RNases and two nonspecific RNases.
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D003460:Crystallization; D012260:Ribonucleases / Q000302:isolation & purification* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D012260:Ribonucleases
vernacular_title: Kompleksnoe vydelenie vnutrikletochnykh i venekletochnykh RNKaz Aspergillus clavatus.