Comparative toxicity of aminoglycoside antibiotics towards the lysosomes in a cell culture model.
Cultured rat fibroblasts were used to compare the cellular toxicity of 4 aminoglycosides: accumulation of the antibiotic in the lysosomes, its adverse effect on phospholipases and consequently the intralysosomal accumulation of phospholipids. Gentamicin and Tobramycin are more noxious than Netilmicin and Amikacin. These data, when corrected for the lesser capture of Tobramycin by the kidney proximal tubules, closely match the toxicological evaluation of these aminoglycosides on the basis of animal and clinical studies.
D000617:Aminoglycosides / Q000633:toxicity; D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects; D008247:Lysosomes / Q000187:drug effects*; D008954:Models, Biological; D051381:Rats; D013108:Sphingomyelin Phosphodiesterase / Q000032:analysis; D013997:Time Factors
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D013108:Sphingomyelin Phosphodiesterase