Evaluation of fluorescence polarization of human blood lymphocytes (SCM test) in the diagnosis of cancer.
Fluorescence polarization was performed on 82 individuals: 32 patients with malignant diseases, 30 apparently healthy individuals, and 20 patients with nonmalignant diseases. The blood lymphocytes from these cases were isolated and challenged with cancer basic protein (CaBP). Stimulation occurred in the majority of the cases of malignant disease (27 of 32). The 30 apparently healthy individuals showed no response to CaBP. Of the 20 with nonmalignant diseases, 18 showed no stimulation by the antigen.
D000328:Adult; D000368:Aged; D000936:Antigen-Antibody Complex / Q000032:analysis; D000951:Antigens, Neoplasm / Q000276:immunology; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005260:Female; D005454:Fluorescence Polarization; D006801:Humans; D008214:Lymphocytes / Q000032:analysis* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins; D009369:Neoplasms / Q000097:blood / Q000175:diagnosis*
D000936:Antigen-Antibody Complex; D000951:Antigens, Neoplasm; D009363:Neoplasm Proteins; C029664:cancer basic protein, human