title: Quantitative determination of fecal bile acids as their methyl ether methyl esters by the repetitive scan technique.
abstract: Quantification of bile acids has been carried out by selected ion monitoring and by repetitive scanning. The latter method has been explored critically and found to be very suitable. A 24 h collection of feces was submitted to Soxhlet extraction. The extract was purified on Servachrom XAD-2, and separated into sulfated and non-sulfated fractions over DEAP-Sephadex-LH20. Conjugated and sulfated bile acids were enzymatically deconjugated and desulfated prior to permethylation. Deuterated bile acid derivatives were added as external standards and an aliquot was injected onto a capillary column of SE-52. The reconstructed chromatogram traces of specific ions were integrated to quantify known bile acids. Spectra of all metabolites were available for identification purposes simultaneously.
mesh_terms: D001647:Bile Acids and Salts / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas / Q000379:methods; D004952:Esters / Q000032:analysis; D004987:Ethers / Q000032:analysis; D005243:Feces / Q000032:analysis*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods
chemical_list: D001647:Bile Acids and Salts; D004952:Esters; D004987:Ethers