Identification of two Thormählen-positive compounds from melanotic urine by gas chromatography-mass spectrometry.
The isolation of two Thormählen-positive compounds from the urine of a patient with malignant melanoma and the elucidation of their structure by gas chromatography-mass spectometry is described. The compounds were isolated using a poly-N-vinylpyrrolidone column and separated by preparative thin-layer chromatography. After elution they were analyzed by gas chromatography and gas chromatography-mass spectrometry as their trimethylsilyl derivatives and after hydrolysis also as their tert.-butyldimethylsilyl derivatives. The results showed the main Thormählen-positive compound A to be the glucuronide of 5-hydroxy-6-methoxyindole, whereas the minor compound AX appeared to be the glucuronide of its isomer 6-hydroxy-5-methoxyindole.
D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D007211:Indoles / Q000652:urine*; D008113:Liver Neoplasms / Q000556:secondary / Q000652:urine; D008545:Melanoma / Q000652:urine*; D009362:Neoplasm Metastasis
D007202:Indicators and Reagents; D007211:Indoles; C030222:5-hydroxy-6-methoxyindole glucuronide; C030221:6-hydroxy-5-methoxyindole glucuronide