title: Influence of cadmium on the intestinal uptake and absorption of calcium in the rat.
abstract: To determine the effects of cadmium on the intestinal absorption of calcium, everted gut sacs from rats orally dosed with 0, 0.05, 0.5 or 5.0 mg Cd daily for 3 weeks were placed in media containing 4 x 10(-5) M Ca. Tissue content of calcium after 1 hour incubation was approximately 1.5 times greater for the 5-mg Cd dose. Serosal fluid content of calcium was decreased by the 0.5- and 5-mg Cd/day doses. In other experiments, gut sacs were incubated in bathing media containing 4 x 10(-5) Ca and Cd in concentrations of 10(-4) to 10(-2) M. Tissue and serosal fluid uptake of calcium decreased as Cd concentration increased. To determine the effects of cadmium on the accumulation of calcium against a concentration gradient, equimolar concentrations of Cd were placed in the mucosal and serosal fluids. Cadmium was added to the mucosal fluid. The accumulation of calcium was abolished by 1.5 x 10(-4) M Cd while at 10(-6) M Cd the accumulation was decreased to one-third the control value. The results indicate that acute or chronic exposure of the intestine to cadmium decreases the intestinal absorption of calcium.
mesh_terms: D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000187:drug effects*; D007700:Kinetics; D008297:Male; D051381:Rats
chemical_list: D002104:Cadmium; D002118:Calcium