title: Determination of opiates in biological samples by glass capillary gas chromatography with electron-capture detection.
abstract: A gas chromatographic method for the simultaneous determination of morphine, 6-acetylmorphine and codeine in human plasma or blood has been developed. The samples are buffered to pH 9 and extracted on silica columns, cleaned by extraction and finally acylated with pentafluoropropionic anhydride. The derivatives formed are separated on a glass capillary column with falling glass needle injection and electron-capture detection. The choice of the extraction conditions and the preparation of suitable capillary columns are discussed.
mesh_terms: D002849:Chromatography, Gas / Q000379:methods; D003061:Codeine / Q000032:analysis; D006207:Half-Life; D006801:Humans; D009020:Morphine / Q000032:analysis; D009269:Nalorphine / Q000032:analysis; D009294:Narcotics / Q000032:analysis*
chemical_list: D009294:Narcotics; D009020:Morphine; D009269:Nalorphine; D003061:Codeine