title: Glucose uptake into plasma membrane vesicles from the maternal surface of human placenta.
abstract: Glucose uptake into plasma membrane vesicles from the maternal surface of the human placenta was measured with the Millipore filtration technique. Uptake off D-glucose was dependent on the osmolarity of the incubation medium surrounding the vesicles. Uptake of D-glucose exceeded that of L-glucose. The uptake of D-glucose was not enhanced by placing 100 mM NaCl or NaSCN in the medium outside the vesicles (none inside) at the onset of uptake determinations. D-glucose transport was inhibited by cytochalasin B; phloretin, phlorizin, and 1-fluoro-2,4-dinitrobenzene. D-glucose uptake was inhibited by 2-deoxy-D-glucose, 3-O-methyl-D-glucose and to a lesser extent by D-galactose. It was not inhibited by alpha-methyl-D-glucoside. Cytochalasin B binding to the vesicles was 30% inhibited in the presence of 80 mM D-glucose. The results indicate that the system for facilitated transport of D-glucose at the maternal face of the placenta is distinctly different from that on the brush-border membrane of intestine or renal tubule and more closely resembles that of human erythrocyte.
mesh_terms: D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004139:Dinitrofluorobenzene / Q000494:pharmacology; D005260:Female; D005374:Filtration; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007536:Isomerism; D009994:Osmolar Concentration; D010693:Phloretin / Q000494:pharmacology; D010695:Phlorhizin / Q000494:pharmacology; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D012964:Sodium / Q000494:pharmacology
chemical_list: D012964:Sodium; D010695:Phlorhizin; D004139:Dinitrofluorobenzene; D005947:Glucose; D010693:Phloretin