Isolation of an (O,H,Vi)-free immunoprotective antigenic fraction with mannose receptor-like activity from Salmonella typhi.
An antigenic fraction of Salmonella typhi strain TY6S, obtained from a bacterial pellet by washings with 1.5 M NaCl and ultracentrifugation in a 5%-20% sucrose density gradient, induced serum antibodies in rabbits that protected chicken embryos challenged with lethal doses of the virulent microorganism. The antigenic preparation was free of Vi, O, and H antigens. It contained about 85% protein and Rd1P+ lipopolysaccharide. The protein moiety comprised three polypeptides of 45,000, 32,000, and 20,000 daltons, respectively. In addition, the purified preparation exhibited the properties of a bacterial adhesin with receptor-like activity for mannosyl residues, as shown by agglutination of target cells containing these residues at their surfaces.
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D006801:Humans; D007114:Immunization; D008358:Mannose / Q000378:metabolism*; D011817:Rabbits; D011946:Receptors, Antigen / Q000378:metabolism*; D012485:Salmonella typhi / Q000276:immunology* / Q000472:pathogenicity; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D011946:Receptors, Antigen; D008358:Mannose