A simplified plaque assay for influenza viruses in Madin-Darby kidney (MDCK) cells.
A variety of influenza A and B viruses plaque in MDCK cell in trypsin is added only at the time of viral adsorption to the monolayer. Therefore, a conventional soft-agar overlay can be employed without addition of proteolytic enzymes. Plaquing efficiency was comparable to that when embryonated eggs were used to determine infectivity. Finally the method is simple and economical.
D000818:Animals; D002460:Cell Line; D004285:Dogs; D009980:Influenza A virus / Q000254:growth & development*; D007668:Kidney; D009975:Orthomyxoviridae / Q000254:growth & development*; D010948:Viral Plaque Assay / Q000379:methods*