Effect of substrate on freeze-dried and shadowed protein structures.
Muscle thick filaments have been prepared for electron microscopy by a method involving freeze-drying and shadowing. The same method applied to filaments on three different substrates gives rise to quite different appearances. The filaments on one of the substrates, hydrophilic carbon, show new detail not previously observed.
D000538:Aluminum Silicates; D000818:Animals; D002244:Carbon; D003599:Cytoskeleton / Q000648:ultrastructure*; D005612:Freeze Drying; D008854:Microscopy, Electron / Q000379:methods*; D011817:Rabbits; D013048:Specimen Handling / Q000379:methods
D000538:Aluminum Silicates; D002244:Carbon; C011934:mica