Intracellular distribution of rat-liver polyribosomes synthesizing coeruloplasmin.
Three independent approaches (binding of 125I-antibodies to polysomes, cell-free translation of polyadenylated mRNA in a wheat germ system and hybridization of RNA with a specific complementary DNA probe) were used to study the intracellular distribution of polysomes involved in the synthesis of coeruloplasmin as well as of coeruloplasmin mRNA sequences in rat liver. It was shown that only membrane-bound polysomes contain both nascent chains of coeruloplasmin and coeruloplasmin mRNA sequences. The size of coeruloplasmin polysomes is 16-18 monomers/mRNA molecule and their proportion is about 0.4-0.6% of total liver polysomes. The proportion of coeruloplasmin mRNA is 0.0025% of the total polysomal RNA and 0.29% of poly(A)-containing mRNA. These values correspond to coeruloplasmin mRNA concentration about 400-535 molecules per parenchymatous liver cell, 90% of those mRNA molecules were recovered from polysomes while the remaining 10% were from postpolysomal supernatant.
D000818:Animals; D001665:Binding Sites; D002474:Cell-Free System; D002570:Ceruloplasmin / Q000096:biosynthesis* / Q000276:immunology; D003593:Cytoplasm / Q000378:metabolism; D007136:Immunoglobulins / Q000032:analysis; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D011132:Polyribosomes / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D014908:Triticum
D007136:Immunoglobulins; D012333:RNA, Messenger; D002570:Ceruloplasmin