[Regulation of the formation and conversion of intermediate cellooligosaccharides and cellobiose during ezymatic hydrolysis of insoluble cellulose].
A kinetic analysis of the main regularities for formation and consumption of the intermediate metabolites (i.e. cellooligosaccharides and cellobiose) in the course of enzymatic hydrolysis of cellulose has been performed. The boundary relationships between the velocities of the individual steps of hydrolysis controlling the transition of the multienzyme cellulase system to be steady-state with respect to the intermediate metabolites were revealed. Using the kinetic approaches developed here, the values of the steady-state concentrations of insoluble intermediate oligosaccharides were determined experimentally for a number of cellulases from the fungi Trichoderma and Geotrichum. It was shown experimentally that when studying the kinetics of enzymatic hydrolysis of cellulose it is necessary to take into account the structural irregularities of the insoluble substrate even when a practically negligible fraction of it (with respect  to its weight) is converted to soluble products. In other cases the kinetic parameters obtained could not be corresponding to the actual reactivity of the cellulose/cellulase system, reflecting rather a difference in reactivity between the labile (amorphous) and stable (crystalline) fractions of a cellulose substrate.
D002475:Cellobiose / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D004187:Disaccharides / Q000378:metabolism*; D005658:Fungi / Q000201:enzymology*; D005848:Geotrichum / Q000201:enzymology*; D006868:Hydrolysis; D007700:Kinetics; D008433:Mathematics; D003904:Mitosporic Fungi / Q000201:enzymology*; D009844:Oligosaccharides / Q000378:metabolism*; D013045:Species Specificity; D014242:Trichoderma / Q000201:enzymology*
D004187:Disaccharides; D009844:Oligosaccharides; D002475:Cellobiose; D002482:Cellulose; D002480:Cellulase
Zakonomernosti obrazovaniia i raskhodovaniia promezhutochnykh tsellooligosakharidov i tsellobiozy pri fermentativnom gidrolize nerastvorimoĭ tselliulozy.