Early events in guinea pig reticulocyte iron uptake.
Hemolysates, prepared from guinea pig reticulocytes incubated with 59Fe-labelled serum, can be resolved into five peaks utilizing molecular sieve chromatography: ferritin, transferrin, hemoglobin, an Mx 17 000 fraction, and a low molecular weight fraction. The hemoglobin peak also contains a nonhemoglobin component (III-X), demonstrated by heme extraction and by isoelectric focusing. Transferrin, the III-X component and the low molecular weight fraction are the first to accumulate radioactive iron during the reticulocyte incubation. The 59Fe in each of these also chases. Therefore, a role for these components as precursors to iron incorporation into heme is suggested.
D000818:Animals; D001692:Biological Transport; D002850:Chromatography, Gel; D005293:Ferritins / Q000097:blood; D006168:Guinea Pigs; D006454:Hemoglobins / Q000378:metabolism; D007501:Iron / Q000097:blood*; D007700:Kinetics; D012156:Reticulocytes / Q000378:metabolism*; D014168:Transferrin / Q000378:metabolism
D006454:Hemoglobins; D014168:Transferrin; D005293:Ferritins; D007501:Iron