Interaction of acrylonitrile with hepatic microsomes of rats and men.
Acrylonitrile (AN) showed spectral interaction with hepatic microsomes from mouse, rat and man. Whereas human and rat liver microsomes resulted in ligand AN-binding spectra, mouse liver microsomes behaved differently. Hepatic microsomes from phenobarbital-treated mice with AN showed a type I substrate binding; ligand spectra are recorded with microsomes from benzo[a]pyrene-treated mice. Microsomes from untreated control mice showed a mixed type behaviour.
D000181:Acrylonitrile / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D019388:Cytochrome P-450 CYP1A2; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008024:Ligands; D051379:Mice; D008810:Mice, Inbred C57BL; D008862:Microsomes, Liver / Q000378:metabolism*; D009570:Nitriles / Q000378:metabolism*; D051381:Rats; D013045:Species Specificity; D013053:Spectrophotometry
D003580:Cytochromes; D008024:Ligands; D009570:Nitriles; D003577:Cytochrome P-450 Enzyme System; C017170:Cyp1a2 protein, rat; D019388:Cytochrome P-450 CYP1A2; D000181:Acrylonitrile