title: Estrogen receptor in rat thymus cytosol.
abstract: Estrogen binding components were characterized in cytosol fractions from the lymphatic tissues of castrated rats. Sucrose gradient analysis revealed specific binding of tritiated estrogen in the thymus. The binding was highly specific since it was easily displaced by unlabeled estrogen, but not by the non-estrogenic steroids used. In thymus cytosols from both males and females, the equilibrium dissociation constant of estrogen binding was--0.3 nM and the saturation binding was 6 fmols/mg of protein. Enzyme- and heat-experiments demonstrated a specific estrogen binder in thymic cytosol which was heat-labile and protein in nature. It was concluded that the rat thymus contains on estrogen receptor which is in part protein and heat-labile.
mesh_terms: D000818:Animals; D001665:Binding Sites; D002369:Castration; D003600:Cytosol / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D008198:Lymph Nodes / Q000378:metabolism; D008297:Male; D051381:Rats; D011960:Receptors, Estrogen / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism*
chemical_list: D011960:Receptors, Estrogen; D004958:Estradiol