Resistance to caffeine of mouse fibroblasts after acquisition of an infinite division potential.
Mouse fibroblasts can easily become permanent cell lines when subcultivated in vitro. It has been previously questioned whether the cells that establish in culture originate in vitro or are already present in the primary culture. We have found that cells from the primary culture do not survive in the presence of caffeine but that after establishment the cells become resistant to the drug. The results suggest that the cells with an infinite growth potential are not present in the primary culture.
D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D002455:Cell Division; D002478:Cells, Cultured; D004351:Drug Resistance; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D008168:Lung / Q000166:cytology; D051379:Mice; D013997:Time Factors
D002110:Caffeine