Mouse glomerular culture.
A method is described for the culture of isolated intact mouse glomeruli. Cultures showed adherent glomeruli after 2 days and from 2-4 days a monolayer of cells began to develop around the points of adherence. The predominant cell (Type I) was 80-200 micrometer in diameter with cytoplasmic extensions, later becoming polyhedral and often multinucleated. Type II spindle cells, 70-100 micrometer in length, were less prominent in the earlier stages and later formed sheaves between Type I cells. Small phagocytic Type III cells, 10-20 micrometer in diameter, were not prominent in cultures from normal kidneys and tended to form small clusters on top of the Type I cell monolayer.
D000818:Animals; D002465:Cell Movement; D046508:Culture Techniques / Q000379:methods; D007678:Kidney Glomerulus / Q000166:cytology*; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D010587:Phagocytosis; D013997:Time Factors