[Estimations of pregnanetriolone, pregnanetetrol, delta 5-pregnenetriol by spectrophotometry and by gas liquid chromatography (author's transl)].
The author describes two methods of simultaneous estimation of pregnanetriolone, pregnanetetrol and delta 5-pregnenetriol. These estimations may be carried out at the same time as those of pregnanediol and pregnanetriol. The first method is based on spectrophotometry of derivatives obtained with 80 % sulphuric acid. The second demands gas chromatography. It permitted us to verify the specificity of the first. Its specificity was also verified by coupling with mass spectrometry. These estimations are used in the diagnosis of deficiencies of 21 hydroxylase and 3-beta hydroxysteroid oxidoreductase from the adrenal cortex. The values frequently obtained for delta 5-pregnenetriol are given.
D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D011279:Pregnanetriol / Q000031:analogs & derivatives* / Q000652:urine; D011283:Pregnenes / Q000652:urine*; D013053:Spectrophotometry / Q000379:methods; D013056:Spectrophotometry, Ultraviolet
D011283:Pregnenes; C016693:5 beta-pregnane-3 alpha,11 beta,17,20 alpha-tetrol; D011279:Pregnanetriol; C009030:pregnanetriolone; C026087:5-pregnene-3 beta,17 alpha,20 alpha-triol
Dosages de prégnanetriolone, prégnanetétrol, delta 5-prégnènetriol par spectrophotométrie et par chromatographie gaz-liquide.