Determination of plasma and urine levels of a new anti-inflammatory agent, 4,5-bis-(4-methoxyphenyl)-2-(2-hydroxymethylsulfinyl)-imidazole, by high-performance liquid chromatography and electrochemical or ultraviolet detection.
The determination of a new anti-inflammatory substance, 4,5-bis-(4-methoxyphenyl)-2-(2-hydroxyethylsulfinyl)-imidazole, in plasma and urine by high-performance liquid chromatography is described. Ultraviolet and electrochemical detection modes are compared and special consideration is given to the mechanism of the electrochemical reaction. The site of oxidation in the molecule seems to be an aliphatic hydroxyl group yielding a carboxyl function in a four-electron transfer reaction. Plasma levels and urinary excretion after an oral dose of 250 mg to two male volunteers have been measured.
D000328:Adult; D000893:Anti-Inflammatory Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry / Q000379:methods; D006801:Humans; D007093:Imidazoles / Q000032:analysis*; D008297:Male; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000893:Anti-Inflammatory Agents; D007093:Imidazoles; C029631:4,5-bis(4-methoxyphenyl)-2-(2-hydroxyethylsulfinyl)imidazole