Protective effect of anionic cholecystographic agents against phalloidin on isolated hepatocytes by competitive inhibition of the phallotoxin uptake. Comparison of the influence on the inward transport of 3H-demethylphalloin and of 14C-cholate.
Several anionic substances used for cholecystography inhibit the development of protrusions in isolated hepatocytes in response to phalloidin. Drugs from the  iopodate family were equieffective with those of th iodipamide type. The above protective effect results from a competitive inhibition of the phallotoxin uptake as shown for iopodate. Cholecystographic agents similarly inhibit the inward transport of cholic acid in a competitive manner. The inhibition of the phallotoxin response is inversely correlated with the uptake of 3H-demethylphalloin (r = 0.94) and with the inward transport of cholate (r = 0.84) at various inhibiting concentrations of iopodate.
D000470:Alkaloids / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism; D001667:Binding, Competitive; D002765:Cholecystography; D002793:Cholic Acids / Q000037:antagonists & inhibitors / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D007458:Iodipamide / Q000378:metabolism* / Q000494:pharmacology; D007487:Ipodate / Q000378:metabolism* / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D009842:Oligopeptides / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D010590:Phalloidine / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats
D000470:Alkaloids; D001647:Bile Acids and Salts; D002793:Cholic Acids; D009842:Oligopeptides; D010590:Phalloidine; C000880:desmethylphalloin; D007487:Ipodate; D007458:Iodipamide