title: Intranuclear location of the tRNA splicing enzymes.
abstract: The intracellular location of the enzymes that excise tRNA intervening sequences and subsequently religate the molecular was investigated in Xenopus oocytes. All the splicing activity detected in a whole oocyte was present in the nucleus. By manual separation of the nuclear envelope and the nuclear contents (which form a gel-like structure in the presence of Mg++) it was found that all the activity of both splicing enzymes was located in the nuclear contents and was not associated with the nuclear membrane.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology* / Q000648:ultrastructure; D005260:Female; D009685:Nuclear Envelope / Q000201:enzymology; D009694:Nucleic Acid Precursors / Q000378:metabolism*; D009865:Oocytes / Q000648:ultrastructure; D012343:RNA, Transfer / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D009694:Nucleic Acid Precursors; D012343:RNA, Transfer; D012260:Ribonucleases