Simple technique for separation of concentrated immunoglobulin M fractions by multi-sample gel chromatography and its application to rubella serodiagnosis.
A simple technique has been developed with which five serum specimens can be chromatographed at one time and concentrated immunoglobulin M fractions can be obtained without using fraction collectors and ultraviolet monitors. When applied to the detection of rubella immunoglobulin M antibody, this technique gave results comparable to the sucrose gradient centrifugation method.
D000914:Antibodies, Viral / Q000032:analysis; D002850:Chromatography, Gel / Q000379:methods; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis / Q000302:isolation & purification*; D012409:Rubella / Q000175:diagnosis*; D012412:Rubella virus / Q000276:immunology; D012698:Serologic Tests
D000914:Antibodies, Viral; D007075:Immunoglobulin M