High-performance liquid chromatography of bile pigments: application to the determination of unconjugated bilirubin in serum.
A rapid, sensitive and specific method for the determination of unconjugated bilirubin in serum is described. Reversed-phase chromatography on short-chain alkylsilica (C2) or octadecylsilica (C18) with acetonitrile--dimethylsulphoxide--water as eluent is used for the separation of bilirubin. The method is applied to the analysis of bilirubin in patients with disorders associated with jaundice.
D000743:Anemia, Hemolytic / Q000175:diagnosis; D001654:Bile Pigments / Q000097:blood; D001663:Bilirubin / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007565:Jaundice / Q000097:blood / Q000175:diagnosis; D008107:Liver Diseases / Q000175:diagnosis
D001654:Bile Pigments; D001663:Bilirubin