The influence of ascorbic acid and DL-alpha-tocopherol on the formation of nitrosamines in an in vitro gastrointestinal model system.
The nitrosation of aminopyrine (4.3 mmol/l) and dimethylamine (4.3 mmol/l) by nitrite (4.3 mmol/l) were studied over the pH range 6-8 in a system containing bile acids and lipid. Both amines were nitrosated to form N-nitrosodimethylamine. The nitrosation of aminopyrine and, to a greater extent, dimethylamine, is enhanced by bile acids. The simultaneous incorporation of ascorbic acid (8.6 mmol/l) and dl-alpha-tocopherol (4.3 mmol/l) was found to be effective in preventing the nitrosation of these two amines. dl-alpha-Tocopheryl acetate was found to be an ineffective blocking agent in this in vitro system.
D000632:Aminopyrine; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D001647:Bile Acids and Salts / Q000502:physiology; D004064:Digestive System / Q000187:drug effects / Q000378:metabolism*; D004123:Dimethylamines; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D009573:Nitrites; D009602:Nitrosamines / Q000378:metabolism*; D014810:Vitamin E / Q000494:pharmacology*
D001647:Bile Acids and Salts; D004123:Dimethylamines; D009573:Nitrites; D009602:Nitrosamines; D000632:Aminopyrine; D014810:Vitamin E; D001205:Ascorbic Acid