title: Changes in energy charge and block of protein synthesis in rabbit reticulocytes under the action of valinomycin.
abstract: Valinomycin blocks protein synthesis at the elongation stage in intact reticulocyte while being without action on a cell free system. We demonstrate here that this membrane mediated inhibition is accompanied by a change of the energy charge (ATP/ADP/AMP ratio) of the cells. There seems to be an accumulation of AMP without a proportional gain in ADP. The change in energy charge in itself can be the cause of the inhibition of protein synthesis. We show also that valinomycin can cause a decrease of the energy charge at concentrations where its action as an ionophore is not seen and independent of a possible activation of the Na-K dependent ATPase.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001798:Blood Proteins / Q000096:biosynthesis*; D043844:Crown Ethers; D004734:Energy Metabolism / Q000187:drug effects*; D004988:Ethers, Cyclic / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000378:metabolism; D066298:In Vitro Techniques; D010042:Ouabain / Q000494:pharmacology; D010441:Peptide Chain Elongation, Translational / Q000187:drug effects*; D011188:Potassium / Q000502:physiology; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects*; D014634:Valinomycin / Q000494:pharmacology*
chemical_list: D001798:Blood Proteins; D043844:Crown Ethers; D004988:Ethers, Cyclic; C002570:dicyclohexyl-18-crown-6; D014634:Valinomycin; D010042:Ouabain; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D011188:Potassium