title: Isolation, identification, and biological activity of 25-hydroxy-24-oxovitamin D3: a new metabolite of vitamin D3 generated by in vitro incubations with kidney homogenates.
abstract: A metabolite of 25-hydroxyvitamin D3 has been isolated in pure form from incubation mixtures containing kidney homogenates of chicks given large doses of vitamin D3. The isolation involved methanol--chloroform extraction and six steps of column chromatography. The metabolite was identified as 25-hydroxy-24-oxovitamin D3 by means of ultraviolet absorption spectrometry, mass spectrometry, infrared spectrometry, nuclear magnetic resonance spectrometry, and specific chemical reactions. Use of a sensitive in situ technique revealed that 25-hydroxy-24-oxovitamin D3 enhances intestinal calcium transport in rats approximately as effectively as 24,25-dihydroxyvitamin D3 does. In contrast, 25-hydroxy-24-oxovitamin D3 appeared to be less active than 24,25-dihydroxyvitamin D3 in chicks 24 h after intravenous injection.
mesh_terms: D000818:Animals; D002112:Calcifediol / Q000031:analogs & derivatives; D002118:Calcium / Q000378:metabolism; D002645:Chickens; D006887:Hydroxycholecalciferols / Q000302:isolation & purification* / Q000378:metabolism* / Q000494:pharmacology; D007408:Intestinal Absorption / Q000187:drug effects; D007668:Kidney / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D013057:Spectrum Analysis
chemical_list: D006887:Hydroxycholecalciferols; C026582:25-hydroxy-24-oxocholecalciferol; D002112:Calcifediol; D002118:Calcium