Sugar derivatives and liver mitochondria. V - Effect of 6-O-benzoyl-3-deoxy-3-fluoro-1,2-O-isopropylidene-alpha-D-glucofuranose.
Using conventional polarographic and mannometric techniques for assaying oxidative phosphorylation and oxygen uptake in isolated rat liver mitochondria, it was observed a decrease in ADP:O ratio and RC coefficient as a function of treatment with 6-O-benzoyl-3-deoxy-3-fluoro-1,2-O-isopropylidene-alpha-D-glucofuranose, using succinate and alpha-ketoglutarate as substrates. The enzymes activities of different segments of the respiratory chain, indicated an inhibition of NADH-oxidase activity as a result of treatment with 6-O-benzoyl-3-deoxy-fluoro-1,2-O-isopropylidene-alpha-D-glucofuranose. The oxidation of NADH-linked substrates by rat liver mitochondria was depressed by the compound used, while the succinate oxidation was increased.
D000818:Animals; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000031:analogs & derivatives / Q000494:pharmacology*; D066298:In Vitro Techniques; D008365:Manometry; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010084:Oxidation-Reduction; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D011506:Proteins / Q000378:metabolism; D051381:Rats
D003837:Deoxy Sugars; D009097:Multienzyme Complexes; D011506:Proteins; C029249:6-O-benzoyl-3-deoxy-3-fluoro-1,2-O-isopropylidene-alpha-D-glucofuranose; D003847:Deoxyglucose; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases