[Template activity of chromatin preparations from cerebellum and large hemispheres of rat brain].
Two preparations of chromatin were isolated frm two divisions of rat brain differing in the average content of RNA in the cells and in the activities of nuclear forms of RNA-polymerases. Cerebellar chromatin was shown to contain far fewer non-histone proteins than the chromatin from the large hemispheres. The template properties of chromatin preparations were studied in a RNA synthesis system by two different methods, using bacterial RNA-polymerase. No differences in the template activity of the two preparations were revealed. It was assumed that the rate of RNA synthesis in these two divisions of rat brain are predominantly due to the activity of nuclear RNA-polymerases rather than to the state of the corresponding matrix.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002531:Cerebellum / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D007700:Kinetics; D051381:Rats; D013698:Templates, Genetic
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D012321:DNA-Directed RNA Polymerases
Matrichnaia aktivnost' preparatov khromatina mozzhechka i bol'shikh polushariĭ mozga krys.