title: [Kinetic properties of alkaline ribonuclease from rat brain].
abstract: The rate of hydrolysis of polymeric yeast RNA by highly purified preparation of alkaline RNAse from the post-mitochondrial cellular fraction from the large hemispheres of rat brain was studied. It was shown that the reaction rate is maximal at the substrate concentration of 1-2 mg/ml. The curve of the reaction rate versus substrate concentration was found to be S-shaped. The value of S0,5 was equal to 0,138 mg/ml. The inhibition of alkaline RNAse by its specific protein inhibitor was of a non-competitive type.
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism*
chemical_list: D012260:Ribonucleases
vernacular_title: Nekotorye kineticheskie svoistva shchelochnoĭ ribonukleazy golovnogo mozga krys.