Evaluation of methods for measurement of steroid hormone receptors.
Several factors affecting estrogen receptor assays have been investigated. Pools of rabbit uterus cytosol and human tumor cytosol were utilized to minimize sampling error. Optimum concentration of diethylstilbestrol was found to be 1000 times the highest labeled ligand concentration. Dithiothreitol (DTT) and ionic strength had no effect on receptor value in human tumor cytosols. Optimum pH for estrogen receptor assay is 7.78.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003600:Cytosol / Q000032:analysis*; D004054:Diethylstilbestrol / Q000378:metabolism; D004229:Dithiothreitol; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009369:Neoplasms / Q000032:analysis; D011817:Rabbits; D011869:Radioligand Assay / Q000379:methods*; D011960:Receptors, Estrogen / Q000032:analysis*; D014325:Tromethamine; D014599:Uterus / Q000032:analysis
D011960:Receptors, Estrogen; D014325:Tromethamine; D004054:Diethylstilbestrol; D004229:Dithiothreitol