The identification of cytochrome P-450-LM2 synthesized in vitro from a rabbit liver polysomal mRNA template.
We demonstrate the in vitro synthesis of cytochrome P-450-LM2 (phenobarbital-inducible form of liver microsomal cytochrome P-450) from a rabbit liver polysomal mRNA template by specific immunoprecipitation of the product. The in vitro synthesized cytochrome P-450-LM2 comigrates with authentic cytochrome P-450-LM2 on sodium dodecyl sulphate-polyacrylamide gels, and is also selectively competed by authentic purified cytochrome P-450-LM2. In addition, we demonstrate that phenobarbital increases the amount of translatable mRNA for cytochrome P-450-LM2 but not for albumin, suggesting that phenobarbital has selective effects on the amount of available translatable mRNA or on mRNA biosynthesis.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D013698:Templates, Genetic
D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital