title: Hemopoiesis in demineralized bone allografts.
abstract: Demineralized allogeneic bone matrix (DBM), implanted in muscle, induces the formation of an ossicle within which histologically recognizable hemopoietic tissue develops. Analyses of rabbit ossicle marrow in a methylcellulose culture system demonstrated the presence of committed hemopoietic precursors; colony-forming units in culture (CFUC), erythroid colony-forming units (CFUE) and erythroid burst-forming units (BFUE) by six weeks post-implantation. The time courses of colony and burst formation by progenitor cells of ossicle and femoral marrow were similar. Induction of hemolytic anemia by phenylhydrazine hydrochloride at six weeks post-DBM implantation showed that the ossicle marrow was responsive to systemic erythropoietic stimuli. The DBM implant may provide a unique model for studying the development of hemopoietic microenvironments within bone.
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells; D001857:Bone Matrix / Q000637:transplantation*; D001842:Bone and Bones / Q000033:anatomy & histology; D006410:Hematopoiesis; D008297:Male; D008903:Minerals / Q000378:metabolism*; D011817:Rabbits; D013997:Time Factors; D014184:Transplantation, Homologous
chemical_list: D008903:Minerals