A novel technique for quantitative autoradiography of labeled histological specimens.
A novel quantitative method is described that makes it possible to routinely and accurately quantify contact autoradiographs of tissue sections in terms of actual amounts of radioactivity per square millimeter. Such determinations can either be made on the entire specimen or on any selected area of arbitrary shape that might correspond to a particular histological structure. This method utilizes the tissue specimens themselves as internal standards and requires the availability of a computerized image analyzer.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001923:Brain Chemistry; D003201:Computers; D006651:Histocytochemistry / Q000379:methods*; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D011856:Radiographic Image Enhancement; D051381:Rats
D009419:Nerve Tissue Proteins