Mechanism of salicylate hydroxylase-catalyzed decarboxylation.
Salicylate hydroxylase (salicylate, NADH: oxygen oxidoreductase (1-hydroxylating, decarboxylating), EC 1.14.13.1) in Pseudomonas putida catalyzed hydroxylation of the substrate analogue, salicylaldehyde, to form catechol and formate with stoichiometric consumption of NADH and O2. Consequently, a study of primary product derived from the carboxyl group of the authentic substrate, salicylate, was undertaken. The experimental results revealed that CO2 not H2CO3, was produced first.
D000332:Aerobiosis; D000447:Aldehydes; D000693:Anaerobiosis; D007700:Kinetics; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology*; D012459:Salicylates / Q000378:metabolism; D013379:Substrate Specificity
D000447:Aldehydes; D012459:Salicylates; C013243:salicylaldehyde; D006899:Mixed Function Oxygenases; C022619:salicylate 1-monooxygenase