Ontogenetic development of high potassium- and acetylcholine-induced release of dopamine from striatal slices of the rat.
The high K+- and acetylcholine (ACh)-induced release of [3H]dopamine (DA) were investigated in whole brain slices of fetuses and striatal slices of postnatal rats. A high K+-induced, Ca2+-dependent release of [3H]DA was demonstrated in brain on day 18 of gestation and the amount of release from the striatum increased during postnatal development. ACh induced [3H]DA release in striatal slices from 3-, 7- and 70-day-old rats but not from 1-day-old animals. ACh-induced [3H]DA release on both day 7 and day 70 was dependent on Ca2+ and significantly reduced by the addition of 5 x 10(-6) M atropine or 10(-5) M hexamethonium. It is suggested that DA is taken up into functionally releasable pools at a late fetal stage and that from around 3 days after birth, ACh may begin to regulate DA release by stimulating muscarinic and/or nicotinic receptors located on DA nerve terminals in the striatum.
D000109:Acetylcholine / Q000494:pharmacology*; D000375:Aging; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D005260:Female; D005865:Gestational Age; D006584:Hexamethonium Compounds / Q000494:pharmacology; D008297:Male; D011188:Potassium / Q000494:pharmacology*; D011247:Pregnancy; D051381:Rats
D006584:Hexamethonium Compounds; D001285:Atropine; D000109:Acetylcholine; D011188:Potassium; D002118:Calcium; D004298:Dopamine