Kainate lesion dissociates striatal dopamine receptor radioligand binding sites.
Kainic acid lesion of rat striata reduces the specific dopamine receptor binding of the butyrophenone antagonist [3H]spiperone and the butyrophenone-like antagonist [3H]domperidone by 56% and 59% respectively. Significantly greater decreases in binding were observed with the agonist [3H]N-propylnorapomorphine (NPA) and the antagonist [3H]flupentixol which showed 79% and 73% losses of high affinity binding respectively. These data indicate that, in part, [3H]spiperone and [3H]domperidone label distinct dopamine receptors with different neuronal localizations from those labeled by [3H]flupentixol and [3H]NPA. Our data is consistent with the hypothesis that [3H]flupentixol and [3H]NPA bind preferentially to adenylate cyclase-linked dopamine (D1) receptors.
D000818:Animals; D001058:Apomorphine / Q000031:analogs & derivatives / Q000378:metabolism; D001562:Benzimidazoles / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism*; D004294:Domperidone; D005475:Flupenthixol / Q000378:metabolism; D066298:In Vitro Techniques; D007608:Kainic Acid / Q000494:pharmacology*; D008297:Male; D010880:Piperidines / Q000378:metabolism; D011759:Pyrrolidines / Q000494:pharmacology*; D011869:Radioligand Assay; D051381:Rats; D011954:Receptors, Dopamine / Q000187:drug effects*; D013134:Spiperone / Q000378:metabolism
D001562:Benzimidazoles; D010880:Piperidines; D011759:Pyrrolidines; D011954:Receptors, Dopamine; D013134:Spiperone; D004294:Domperidone; C009074:N-n-propylnorapomorphine; D005475:Flupenthixol; D001058:Apomorphine; D007608:Kainic Acid