Comparison of quantitation methods for L-1210 cell populations and evaluation of selected cytotoxic agents in leukemic mice.
A more rapid cell-counting technique using an electronic cell counter was developed as an improvement over the slower hemocytometer method. The electronic counting method produced cell counts that had a smaller standard deviation but were not significantly different from the hemocytometer method. The acetate, hexanoate, and decanoate esters of p-N,N-bis(2-chloroethyl)aminophenol were investigated for acute toxicity in mice, effects on survival times, and effects on L-1210 cell populations in L-1210 leukemic mice. The decanoate ester was the least toxic compound and was most effective in lengthening the lifespan of the mice. The acetate and hexanoate esters were more effective in reducing L-1210 cell populations.
D000816:Aniline Mustard / Q000031:analogs & derivatives / Q000627:therapeutic use / Q000633:toxicity; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use* / Q000633:toxicity; D002452:Cell Count / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical; D007928:Lethal Dose 50; D007939:Leukemia L1210 / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains
D000970:Antineoplastic Agents; D000816:Aniline Mustard