The complete neuraminidase of influenza A/PR8/34 (H0N1) is not detectable in its recombinant virus.
The neuraminidase located on the influenza virus recombinant A/Eq (Heq1) x A/PR8 (N1) was relatively a poor antigen (NI titre less than 10) as compared with the enzyme present on the parent virus A/PR8/34 (H0N1) (NI titre 160). This difference in the antigenic behavior of the neuraminidase of A/PR8/34 (H0N1) virus was explored. Results obtained showed that rabbit anti-A/PR8/34 (H0N1) serum contained two distinct types of antineuraminidase antibody and only one type of antineuraminidase antibody was precipitable by the neuraminidase located on the recombinant virus. Evidence suggests that the parent enzyme(s) is not detectable in its entirety on its recombinant virus.
D000956:Antigens, Viral / Q000032:analysis*; D009980:Influenza A virus / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D009439:Neuraminidase / Q000276:immunology*; D011995:Recombination, Genetic
D000956:Antigens, Viral; D009439:Neuraminidase