Collagen gels as a matrix for haemopoiesis.
We have shown that collagen gel can be used as a culture matrix for the cloning of granulocyte/macrophage progenitor cells (CFU-C), the production of foci of marrow stromal cells and the maintenance of stem cell proliferation, differentiation and the production of CFU-C. Since collagen is a physiological matrix and allows the simultaneous growth of a variety of cellular elements, the system should prove useful for examining the role of cell/cell interactions and regulatory molecules involved in haemopoiesis.
D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen; D003114:Colony-Forming Units Assay; D005782:Gels; D006098:Granulocytes / Q000502:physiology; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D008264:Macrophages / Q000502:physiology; D051379:Mice; D013997:Time Factors
D005782:Gels; D003094:Collagen