13C nuclear magnetic resonance studies of the composition and fluidity of several chloroplast monogalactosyldiacylglycerols.
The motional properties of four monogalactosyldiacylglycerols isolated from photosynthetic membranes, and containing different fatty acid chain lengths and degrees of unsaturation, have been determined using 13C nuclear magnetic resonance. These properties have been compared with those of a lipid containing only saturated fatty acids. The 13C longitudinal relaxation times (T1) of the carbon atoms of the acyl chains in [2H4] methanol were measured as an index of the rates of motion of the lipid molecules and used to compare the relative fluidity of the acyl chains. The T1 values of the glyceryl and galactosyl carbon atoms in each monogalactosyldiacylglycerol are essentially constant, when allowance is made for concentration differences and the presence of two hydrogens on a methylene carbon versus one on a methine carbon. These results indicate similar rates of motion for the headgroup carbons in each lipid. However, for the acyl chains, the T1 values increase with the introduction of a double bond and increase further with additional unsaturation. This increase in the rate of motion only occurs at carbon atoms beyond the first double bond in an acyl chain. These results differ to those reported for monolayer experiments where changes in packing characteristics are predominantly dependent on the introduction of the first double bond and then vary little between species.
D002736:Chloroplasts / Q000032:analysis*; D004075:Diglycerides / Q000032:analysis* / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis; D038983:Galactolipids; D005989:Glycerides / Q000032:analysis*; D006017:Glycolipids / Q000032:analysis* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008560:Membrane Fluidity
D004075:Diglycerides; D005227:Fatty Acids; D038983:Galactolipids; D005989:Glycerides; D006017:Glycolipids; C009909:monogalactosyldiacylglycerol