Studies of the IgM and IgA contamination obtained by eluting IgG from protein A-Sepharose column with pH steps.
The IgG fraction obtained from pooled human plasma by eluting a protein A column with a buffer at pH 2.5 was contaminated with approximately 30% of the IgM originally present in the sample. Both the IgM and the IgA contamination can be reduced and the IgG recovery maintained at 90% of the bound IgG when elution of the column is performed at pH 4.0
D002846:Chromatography, Affinity; D006801:Humans; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007075:Immunoglobulin M / Q000302:isolation & purification; D013205:Staphylococcal Protein A / Q000276:immunology*
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013205:Staphylococcal Protein A