The regenerating fascial sheath in lipectomized Osborne-Mendel rats: morphological and biochemical indices of adipocyte differentiation and proliferation.
We investigated the cellular and enzymatic changes that occur as adipose tissue regenerates following bilateral lipectomy of Osborne-Mendel rats. At 6, 8, 10, 12 and 14 days after surgery regenerating fascial sheaths regrowing at the site of the inguinal fat depots were fixed in situ and removed for studies of cellular and tissue morphology at both light and electron microscopic levels. Additional tissue from some animals was prepared for measurement of thymidine kinase and lipoprotein lipase activity. Six days after surgery, fascial sheaths had regrown at the site and contained a few mature unilocular adipocytes as well as numerous other cells, some of which contained non-membrane bound lipid droplets, and which were tentatively identified as pre-adipoblasts and adipoblasts. Over the period from day 6 to day 14 the number of unilocular adipocytes in the tissue doubled and the average cell size increased. These changes in cellular morphology were accompanied by correlative changes in enzyme activity.
D000273:Adipose Tissue / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D005205:Fascia / Q000166:cytology*; D006801:Humans; D008071:Lipoprotein Lipase / Q000378:metabolism; D008854:Microscopy, Electron; D051381:Rats; D012038:Regeneration; D013937:Thymidine Kinase / Q000378:metabolism
D013937:Thymidine Kinase; D008071:Lipoprotein Lipase