Cell assembly patterns of embryonic mouse cerebellar cells on carbohydrate-derivatized polylysine culture substrata.
Four carbohydrate derivatives of poly-D-lysine have been synthesized and assayed as substrates for the tissue culture of embryonic mouse cerebellar cells. On poly-beta-(D-glucopyranosyl)-poly-D-lysine and on poly-beta-(N-acetyl-D-glucosaminyl)-poly-D-lysine, dissociated cerebellar cells formed a monolayer. On poly-beta-(D-galactopyranosyl)-poly-D-lysine, cellular aggregates were formed and cables of processes were extended between the aggregates. On poly-beta-(L-fucosyl)-poly-D-lysine, cerebellar cells failed to attach and died within 24 h. On poly-(N-acetyl)-poly-D-lysine, cell attachment was identical to that on poly-D-lysine. At low concentrations of underivatized poly-D-lysine (0.5-2.0 microgram/ml) dissociated embryonic cerebellar cells formed cellular aggregates, whereas at higher concentrations of poly-D-lysine monolayering was extensive.
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology* / Q000187:drug effects; D004622:Embryo, Mammalian; D005685:Galactans / Q000494:pharmacology*; D005936:Glucans / Q000494:pharmacology*; D051379:Mice; D010455:Peptides / Q000494:pharmacology*; D011107:Polylysine / Q000031:analogs & derivatives / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D005685:Galactans; D005936:Glucans; D010455:Peptides; C029670:poly-beta-(D-galactopyranosyl)-poly-D-lysine; C029671:poly-beta-(D-glucopyranosyl)-poly-D-lysine; C029669:poly-beta-(L-fucosyl)-poly-D-lysine; C029672:poly-beta-(N-acetyl-D-glucosaminyl)-poly-D-lysine; D011107:Polylysine