Control of membrane lipids in Mycoplasma gallisepticum: effect on lipid order.
Adaptation of Mycoplasma gallisepticum, a sterol-requiring Mycoplasma sp., to growth in a serum-free medium supplemented with cholesterol in decreasing concentrations and with various saturated or unsaturated fatty acids enabled us to control both the cholesterol levels and the membrane fatty acid composition. An estimate of the membrane physical state from fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene indicated that the membrane lipids of native M. gallisepticum were highly ordered. Elongation of the saturated fatty acid chains from 14 to 18 carbon atoms caused only a small increase in the membrane lipid ordering, whereas the introduction of a cis double bond reduced it significantly. Lipid-phase transitions were observed in low-cholesterol-adapted organisms, whose membrane lipids were still highly ordered at the growth temperature.
D002784:Cholesterol / Q000378:metabolism; D005454:Fluorescence Polarization; D008563:Membrane Lipids / Q000032:analysis*; D009174:Mycoplasma / Q000378:metabolism* / Q000648:ultrastructure
D008563:Membrane Lipids; D002784:Cholesterol