[Respiration and oxidative phosphorylation in isolated liver cells under freezing conditions].
Respiration and oxidative phosphorylation of hepatocytes were studied as affected by low temperatures and cryoprotectors. Fast freezing of these cells down to -196 degrees C with the presence of mentioned substances causes considerable destruction of their structures. The intensity of the oxygen endogenic uptake lowers significantly, respiration and oxidative phosphorylation become uncoupled. Two-stage freezing down to -196 degrees C also causes a complete uncoupling of the oxidation and phosphorylation processes in the hepatocyte mitochondria. When freezing hepatocytes down to -196 degrees C by the multistage programme with the presence of cryoprotectors in a 10% concentration, the mitochondria retain 20, 30 and 40% of their functional properties, respectively.
D000818:Animals; D003486:Cyanides / Q000494:pharmacology; D005615:Freezing; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats
D003486:Cyanides
D'ikhanie i okislitel'noe fosforilirovanie v izolirovannykh kletkakh pecheni pri zamorashivanii.