title: Further production and characterization of antibodies reactive with pyrazolone derivatives.
abstract: A sensitive radioimmunoassay for pyrazolone derivatives has been developed. Anti-antipyrine antisera were produced in rabbits by repeated immunization with 4-succinamidoantipyrine coupled to bovine serum albumin. Less than 1 ng of antipyrine could be detected by this procedure. Various substituents on the carbon-4 position of the pyrazolone ring decreased the affinity for the antibody. The concentrations in ng of various pyrazolone derivatives required to inhibit [3H]antipyrine binding by 50% were: antipyrine, 6.8; aminopropylon, 8.5; sulpyrine, 35.5; isopropylantipyrine, 1320; and aminopyrine, 2820. The antibody showed no cross-reactivity with any other antipyretics such as pyrazolidine or aniline derivatives. The determination of antipyrine and sulpyrine concentrations in rat serum after i.p administration was also carried out.
mesh_terms: D000632:Aminopyrine / Q000097:blood / Q000276:immunology / Q000031:analogs & derivatives; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D000983:Antipyrine / Q000031:analogs & derivatives / Q000097:blood / Q000276:immunology; D001667:Binding, Competitive; D055598:Chemical Phenomena; D002621:Chemistry; D004177:Dipyrone / Q000097:blood / Q000276:immunology; D006241:Haptens / Q000032:analysis; D008297:Male; D011720:Pyrazoles / Q000032:analysis*; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats
chemical_list: D000906:Antibodies; D006241:Haptens; D011720:Pyrazoles; D000632:Aminopyrine; D004177:Dipyrone; C028781:aminopropylon; C009077:propyphenazone; D000983:Antipyrine