Detection of antibodies against circulating cathodic antigen of Schistosoma mansoni using the enzyme-linked immunosorbent assay.
The applicability of a Schistosoma mansoni polysaccharide antigen, circulating cathodic antigen (CCA), for the detection of antibodies in S. mansoni infection was tested in the enzyme-linked immunosorbent assay (ELISA). Antibodies against this secretory antigen were detected in hamster infections after three weeks, while in infected humans anti-CCA antibodies could be demonstrated eight weeks after infection. Antibodies could be demonstrated both in recent and chronic infections in man, but more false-negative results were observed in chronic infections. The antibody response was composed of both IgM and IgG antibodies.
D000328:Adult; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D002648:Child; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007700:Kinetics; D008647:Mesocricetus; D012550:Schistosoma mansoni / Q000276:immunology*; D012552:Schistosomiasis / Q000276:immunology*
D000906:Antibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M