The loosely bound non-histone chromosomal proteins of rat prostate in androgen action.
Rat prostatic chromatin was fractionated by sequential salt extractions into 0.35 M NaCl-soluble (the loosely bound non-histone chromosomal proteins), 2 M NaCl-soluble and -insoluble (the residual proteins) fractions. Homologous and heterologous chromatins were reconstituted with these chromosomal fractions from castrated rats injected with testosterone for 2 h and from castrated control. Chromatin reconstituted with all hormone-treated fractions showed 30% more binding with polylysine and transcriptional template activity than did chromatin reconstituted with castrated fractions. Similar analyses of reconstituted heterologous chromatins indicated that the androgen-induced changes in chromatin were mainly determined by the androgenic state of the 0.35 M NaCl-soluble fraction. The 0.35 M NaCl-soluble fraction also contained 18% more total high mobility group protein than did the corresponding castrated fraction. Limited digestion of 5 alpha-[3H]dihydrotestosterone-bound nuclei with pancreatic DNAase 1 to 5% acid-soluble resulted in a rapid release of the nuclear-bound 3H-labelled androgen and protein, in contrast to similar digestion with microsomal nuclease. Since a large portion of the translocation androgen-receptor is bound to protein(s) in the 0.35 M NaCl-soluble fraction, these results suggest that the component(s) in the 0.35 M NaCl-soluble fraction to which androgen-receptor binds is associated with actively transcribing sequences on chromatin.
D000728:Androgens; D000818:Animals; D002843:Chromatin / Q000302:isolation & purification / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification / Q000378:metabolism*; D013196:Dihydrotestosterone / Q000378:metabolism; D008297:Male; D011467:Prostate / Q000166:cytology / Q000378:metabolism*; D012319:RNA Polymerase II / Q000378:metabolism; D051381:Rats; D011944:Receptors, Androgen / Q000378:metabolism; D013698:Templates, Genetic; D014158:Transcription, Genetic
D000728:Androgens; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D011944:Receptors, Androgen; D013196:Dihydrotestosterone; D012319:RNA Polymerase II