Ethane production of mouse peritoneal macrophages as indication for lipid peroxidation and the effect of heavy metals.
Mouse peritoneal macrophages cultivated for 24 h produced ethane which is a new index for lipid peroxidation reactions, whereas malondialdehyde (MDA), an alternative method, did not increase. This is explained by the metabolism of MDA by macrophages after addition of MDA, whereas the gaseous ethane added remained constant. This indicates that ethane rather than MDA is a reliable parameter for lipid peroxide formation by macrophages. Divalent heavy metal ions were examined for their effect on ethane formation and on cell viability. Whereas the amount of ethane formed by macrophages in presence of CdCl2 and MnCl2 remained fairly constant, PbCl2 and ZnCl2 resulted in decreased ethane production up to 60%. But with all these metals the viability decreased much more and did not correlate with the ethane formation. With some concentrations of FeCl2 an increased ethane formation was observed which was accompanied by decreased viability of the cells. The results are consistent with the view that heavy metals interfere with the oxygen radical reactions of macrophages, and that viability of macrophages is not closely related to these oxidative reactions.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002478:Cells, Cultured; D004980:Ethane / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008670:Metals / Q000633:toxicity*; D051379:Mice
D008054:Lipid Peroxides; D008670:Metals; D004980:Ethane