Use of citrate agar electrophoresis in evaluation of antisickling agents.
Hemoglobin S (Hb S), treated with antisickling agents which interfere with gel formation, has been found to migrate on citrate agar electrophoresis in a manner similar to Hb A or Hb F. This finding suggests a rapid and inexpensive method for screening potential antisickling compounds of the types which interfere with gelation. This investigation has also led to an improved understanding of the molecular basis of citrate agar electrophoresis and to the realization that agar itself may contain a natural antisickling agent.
D000986:Antisickling Agents / Q000494:pharmacology*; D002951:Citrates; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D006451:Hemoglobin, Sickle; D006801:Humans
D000986:Antisickling Agents; D002951:Citrates; D006451:Hemoglobin, Sickle