Effects of DNP on rat thymocyte uptake of 2-deoxyglucose: effects of nutrients.
Rat thymocyte glucose transport activity was measured by the uptake of 3H-2-deoxyglucose. In the presence of 5mM glucose, DNP stimulated the uptake of 2-deoxyglucose. In the absence of glucose, DNP inhibited the uptake of 2-deoxyglucose. Both stimulatory and inhibitor effects of DNP were DNP concentration dependent. The incubation of thymocytes with nutrients which are metabolized through mitochondrial oxidation, such as beta-hydroxybutyrate, pyruvate, alanine and glutamine, did not support the stimulatory effects of DNP on 2-deoxyglucose uptake. The incubation of thymocytes with a nutrient which can support glycolysis, such as adenosine, supported the stimulatory effect of DNP on 2-deoxyglucose uptake. Iodoacetate, an inhibitor of glycolysis, inhibited 2-deoxyglucose uptake even in the presence of glucose. The present data suggested that the stimulatory effect of DNP on thymocyte 2-deoxyglucose uptake required intact glycolysis activity.
D019297:2,4-Dinitrophenol; D000818:Animals; D003470:Culture Media; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D004140:Dinitrophenols / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D066298:In Vitro Techniques; D007461:Iodoacetates / Q000494:pharmacology; D051381:Rats; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors
D003470:Culture Media; D003837:Deoxy Sugars; D004140:Dinitrophenols; D007461:Iodoacetates; D003847:Deoxyglucose; D005947:Glucose; D019297:2,4-Dinitrophenol