Strategies for spectral assignment in the 1H NMR spectra of a 25 000 Mr murine antibody fragment: (i) in vivo deuteration and (ii) use of a denaturant.
D000818:Animals; D001666:Binding Sites, Antibody; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003903:Deuterium; D006146:Guanidines; D007135:Immunoglobulin Variable Region; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008970:Molecular Weight; D011489:Protein Denaturation; D014364:Tryptophan
D006146:Guanidines; D007135:Immunoglobulin Variable Region; D014364:Tryptophan; D003903:Deuterium