Competitive binding studies of compounds that interact with DNA utilizing fluorescence polarization.
The increase in the fluorescence polarization of acridine orange upon binding to DNA molecules is used as the basis of a competitive method to study the interaction of a variety of fluorescent and non-fluorescent compounds with DNA. Test compounds that interact with DNA inhibit both the binding of acridine orange to DNA and the accompanying increase in fluorescence polarization. Actinomycin D exhibits a dose-dependent inhibition of acridine orange-DNA binding with Micrococcus luteus DNA, calf thymus DNA, and poly(dG-dC); no detectable inhibition of acridine orange intercalation into poly(dA-dT) is observed. In contrast, proflavine shows similar acridine orange inhibition for poly(dA-dT), calf thymus DNA and M. luteus DNA.
D000165:Acridine Orange / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D005454:Fluorescence Polarization; D008837:Micrococcus; D011067:Poly dA-dT / Q000378:metabolism; D011089:Polydeoxyribonucleotides / Q000378:metabolism
D004269:DNA, Bacterial; D011089:Polydeoxyribonucleotides; D003609:Dactinomycin; D011067:Poly dA-dT; C019530:poly(dC-dG); D004247:DNA; D000165:Acridine Orange