title: [Establishment of optimal conditions at laboratory and pilot plant levels for the preparation of a protein isolated from Lupinus mutabilis].
abstract: Laboratory conditions were first investigated to determine are optimum processing parameters for the preparation of a protein isolate from the ground, defatted, commercial flakes of Lupinus mutabilis. The extraction variables were: pH (2-10); solvent to lupine ratio (5:1 to 40:1); temperature (28 degrees C - 60 degrees C) and time (10-50 min). The isoelectric point of the lupine protein was found to be pH 4.5 with a protein solubility higher than 80% above pH 8.0. Using 70-100 mesh, ground defatted flakes, and extracting at pH 8.7 for 60 min, a protein isolate was obtained on acidification to pH 4.5 which was 99.8 protein (dry basis), compared to 55.25% protein for the original material. This protein isolate represented 32% of the initial material and 57.6% of the initial nitrogen. When making pilot plant assays we found that the yield of protein isolate decreased to 20.4% of the original material and 36.4% of the initial nitrogen. The protein efficiency ratio for the protein isolate was 2.15 when supplemented with methionine, and had a digestibility of 89.33
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002364:Caseins / Q000032:analysis; D005511:Food Handling; D006863:Hydrogen-Ion Concentration; D008715:Methionine / Q000032:analysis; D010865:Pilot Projects; D010940:Plant Proteins / Q000302:isolation & purification; D012639:Seeds; D013025:Glycine max / Q000032:analysis; D013696:Temperature
chemical_list: D000596:Amino Acids; D002364:Caseins; D010940:Plant Proteins; D008715:Methionine
vernacular_title: Establecimiento de las condiciones óptimas a nivel de laboratorio y de planta piloto para la preparación de un aislado proteínico de Lupinus mutabilis.