Hybridization frequencies of Chinese hamster and mouse fibroblasts fused with polyethylene glycol at different cell densities.
We have determined the effect of parental cell concentration at the time of fusion with polyethylene glycol on the frequency of viable somatic hybrids and of polykaryocytes of different multiplicity, between Chinese hamster (line Wg3) and mouse (line 3TP) fibroblasts. Fusion appeared to be dictated by the random encounter of cells only at low cell concentrations. However, no evidence for species-specific restriction of fusion was obtained at any cell concentration.
D000818:Animals; D002459:Cell Fusion / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D006822:Hybrid Cells / Q000187:drug effects*; D051379:Mice; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013045:Species Specificity
D011092:Polyethylene Glycols