title: The electrophoresis of transferrins in urea/polyacrylamide gels.
abstract: The denaturation of transferrin by urea has been studied by (a) electrophoresis in polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss of iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin and hen ovotransferrin the N-terminal and C-terminal domains of the iron-free protein were found to denature at different urea concentrations.
mesh_terms: D000818:Animals; D002645:Chickens; D003207:Conalbumin; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007501:Iron; D011485:Protein Binding; D011489:Protein Denaturation; D013056:Spectrophotometry, Ultraviolet; D014168:Transferrin; D014508:Urea
chemical_list: D014168:Transferrin; D003207:Conalbumin; D014508:Urea; D007501:Iron